Analysis of protein composition using multidimensional chromatography and mass spectrometry.
Multidimensional liquid chromatography of peptides produced by protease digestion of complex protein mixtures followed by tandem mass spectrometry can be coupled with automated database searching to identify large numbers of proteins in complex samples. These methods avoid the limitations of gel electrophoresis and in-gel digestions by directly identifying protein mixtures in solution.
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Andrew J
AJ
Vanderbilt University School of Medicine, Nashville, Tennessee.
Jennings
Jennifer L
JL
Vanderbilt University School of Medicine, Nashville, Tennessee.
Washburn
Michael P
MP
Torrey Mesa Research Institute, San Diego, California.
D000809:Angiotensins / Q000737:chemistry; D002021:Buffers; D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D012984:Software
D000809:Angiotensins; D002021:Buffers; D011506:Proteins