title: [The effect of aloesin on melanocytes in the pigmented skin equivalent model].
abstract: OBJECTIVE
  To observe the effect of aloesin, tea polyphenols, arbutin on melanocytes in the pigmented skin equivalent model.
  
  
  METHODS
  First, we constructed the pigmented skin equivalent model in vitro. And then we detected the effect of aloesin, tea polyphenols and arbutin on the cells' shape, tyrosinase activity and formation of melanin in the constructed pigmented skin equivalent.
  
  
  RESULTS
  Three depigmenting agents showed an inhibition effect on the tyrosinase activity of melanocytes and reduced significantly melanin content in the pigmented skin equivalent model, in which the tea polyphenols had the strongest effect, and then was the aloesin. But the tea polyphenols showed the strongest toxicity, while the aloesin and arbutin had a much lower toxicity.
  
  
  CONCLUSIONS
  All the three depigmenting agents showed a concentration dependent suppression effect on the tyrosinase activity and formation of melanin, in which the tea polyphenols was the strongest effect( P <0.05). Aoesin has a good suppression effect on the tyrosinase activity and formation of melanin, but has a much lower toxicity, which could be used as a safe depigmenting agent.
authors.affiliation: Department of Oral and Maxillofacial Plastic Surgery, Stomatological College, Xi'an Jiao Tong University, Xi'an 710004, China. yzhqun@mail.xjtu.edu.cn
authors.forename: Zhuang-qun
authors.initials: ZQ
authors.lastname: Yang
authors.forename: Zheng-Hui
authors.initials: ZH
authors.lastname: Wang
authors.forename: Tie-Liang
authors.initials: TL
authors.lastname: Zhang
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Tu
authors.forename: Yong
authors.initials: Y
authors.lastname: Song
authors.forename: Xiao-Yi
authors.initials: XY
authors.lastname: Hu
authors.forename: Guo-Guang
authors.initials: GG
authors.lastname: Li
mesh_terms: D001104:Arbutin / Q000494:pharmacology*; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D052816:Foreskin / Q000166:cytology; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008543:Melanins / Q000096:biosynthesis; D008544:Melanocytes / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D010858:Pigmentation; D059808:Polyphenols; D012867:Skin / Q000187:drug effects; D015595:Skin Aging / Q000187:drug effects
chemical_list: D002867:Chromones; D005419:Flavonoids; D005960:Glucosides; D008543:Melanins; D010636:Phenols; D059808:Polyphenols; D001104:Arbutin; C069868:aloesin