Size-exclusion chromatography with on-line light scattering.
This unit describes the use of size-exclusion chromatography with on-line light scattering, UV absorbance, and refractive index detectors (SEC-LS/UV/RI) to determine: (a) the molecular weight of simple proteins containing no carbohydrates, (b) the molecular weight of glycoproteins, and (c), most importantly, the molecular weight and stoichiometry of protein-protein complexes or protein-carbohydrate complexes. Multiangle light scattering is also discussed.
Arakawa
T
T
Alliance Protein Laboratories, Inc., Thousand Oaks, California, USA.
Wen
J
J
D002850:Chromatography, Gel / Q000379:methods*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism; D008027:Light; D008970:Molecular Weight; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D012542:Scattering, Radiation
D006023:Glycoproteins; D011506:Proteins