Embryonic chicken trachea as a new in vitro model for the investigation of mucociliary particle clearance in the airways.
Mucociliary clearance (MC) is an important defense mechanism of the respiratory system to eliminate inhaled and possibly noxious particles from the lung. Although the principal mechanics of MC seem to be relatively clear there are still open questions regarding the long-term clearance of particles. Therefore, we have developed a new set-up based on embryonic chicken trachea (ECT) to investigate mucociliary particle clearance in more detail. ECT was placed in an incubation chamber after carbon particles were applied and tracked using optical microscopy. The aim of the study was to validate this model by investigating the impact of temperature, humidity and drugs on particle transport rates. Particles were transported reproducibly along the trachea and clearance velocity (2.39 +/- 0.25) mm/min was found to be in accordance to data reported in literature. Variation in temperature resulted in significantly reduced MC: (0.40 +/- 0.12) mm/min (20 degrees C); (0.42 +/- 0.10) mm/min (45 degrees C). Decreasing humidity (99-60%) had no significant effect on MC, whereas reduction to 20% humidity showed a significant influence on particle clearance. The use of different cilio- and muco-active drugs (propranolol, terbutalin, N-acetylcysteine) resulted in altered MC according to the pharmacological effect of the substances: a concentration dependent decrease of MC was found for Propranolol. From our results we conclude that this model can be employed to investigate MC of particles in more detail. Hence, the model may help to understand and identify decisive physico-chemical parameters for MC and to answer open questions regarding the long-term clearance phenomenon.
Henning
A
A
Biopharmaceutics and Pharmaceutical Technology, Saarland University, P.O. Box 151150, 66041 Saarbrücken, Germany.
Schneider
M
M
Bur
M
M
Blank
F
F
Gehr
P
P
Lehr
C-M
CM
D000111:Acetylcysteine / Q000494:pharmacology; D000818:Animals; D002244:Carbon / Q000378:metabolism*; D002642:Chick Embryo; D004305:Dose-Response Relationship, Drug; D006813:Humidity; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D009079:Mucociliary Clearance / Q000187:drug effects; D016612:Paraffin Embedding; D010316:Particle Size; D052638:Particulate Matter / Q000378:metabolism*; D011433:Propranolol / Q000494:pharmacology; D015203:Reproducibility of Results; D013696:Temperature; D013726:Terbutaline / Q000494:pharmacology; D046509:Tissue Culture Techniques; D014132:Trachea / Q000187:drug effects / Q000196:embryology / Q000378:metabolism* / Q000648:ultrastructure
D052638:Particulate Matter; D002244:Carbon; D011433:Propranolol; D013726:Terbutaline; D000111:Acetylcysteine