title: Loss of expression of EphB1 protein in gastric carcinoma associated with invasion and metastasis.
abstract: OBJECTIVE
  EphB1 is a member of the Eph family of receptor tyrosine kinases that is involved in embryonic nervous and vascular system development. Over- or underexpression of certain Eph receptors has been found in some cancer samples compared to normal tissue. Expression of Eph receptors is related to malignant transformation, metastasis, differentiation, and prognosis of cancers. Recently, the EphB subfamily has been shown to be involved in the tumorigenesis of colorectal cancer. In the present study, expression of the EphB1 transcript and protein in gastric carcinoma samples was determined to investigate the roles of EphB1 in development, progress and prognosis of gastric carcinoma.
  
  
  METHODS
  Quantitative real-time reverse transcriptase-polymerase chain reaction and immunohistochemical staining were used.
  
  
  RESULTS
  The EphB1 transcript was overexpressed in 68.9% (42/61) and underexpressed in 14.8% (9/61) of cases. However, the expression of protein was greatly different from the transcript expression, with overexpression and underexpression being 17.2% (10/58) and 44.8% (26/58), respectively. In addition, we showed that underexpression of EphB1 protein is significantly associated with invasion, stage and metastasis in gastric carcinomas.
  
  
  CONCLUSIONS
  EphB1 may have a tumor-suppressive role in gastric cancer.
authors.affiliation: Department of Pathology, Nanjing Jinling Hospital/Nanjing University School of Medicine, Nanjing, PR China.
authors.forename: Jian-dong
authors.initials: JD
authors.lastname: Wang
authors.forename: Ying-chun
authors.initials: YC
authors.lastname: Dong
authors.forename: Zhen
authors.initials: Z
authors.lastname: Sheng
authors.forename: Heng-hui
authors.initials: HH
authors.lastname: Ma
authors.forename: Guo-li
authors.initials: GL
authors.lastname: Li
authors.forename: Xu-lin
authors.initials: XL
authors.lastname: Wang
authors.forename: Guang-ming
authors.initials: GM
authors.lastname: Lu
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Sugimura
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
authors.forename: Xiao-jun
authors.initials: XJ
authors.lastname: Zhou
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000556:secondary*; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007414:Intestinal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D036225:Receptor, EphB1 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D036225:Receptor, EphB1