Characterization of oil bodies in jelly fig achenes.
Thin-layer chromatography analysis revealed that the contents stored in oil bodies isolated from jelly fig (Ficus awkeotsang Makino) achenes were mainly neutral lipids (>90% triacylglycerols and approximately 5% diacylglycerols). Fatty acids released from the neutral lipids of achene oil bodies were highly unsaturated (62.65% alpha-linolenic acid, 18.24% linoleic acid, and 10.62% oleic acid). The integrity of isolated oil bodies was presumably maintained via electronegative repulsion and steric hindrance provided by their surface proteins. Immunological cross-recognition using antibodies against sesame oil-body proteins indicated that two oleosin isoforms and one caleosin were present in these oil bodies. MALDI-MS analyses confirmed that the three full-length cDNA fragments obtained by PCR cloning from maturing achenes encoded the two jelly fig oleosin isoforms and one caleosin identified by immunological screening.
Chua
Anna C N
AC
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 40227, Taiwan.
Jiang
Pei-Luen
PL
Shi
Li-Shian
LS
Chou
Wing-Ming
WM
Tzen
Jason T C
JT
D000595:Amino Acid Sequence; D015153:Blotting, Western; D002855:Chromatography, Thin Layer; D004075:Diglycerides / Q000737:chemistry / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D030681:Ficus / Q000235:genetics / Q000378:metabolism*; D002479:Inclusion Bodies / Q000737:chemistry / Q000378:metabolism*; D019787:Linoleic Acid / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D019301:Oleic Acid / Q000737:chemistry / Q000378:metabolism; D010938:Plant Oils / Q000737:chemistry / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism; D017962:alpha-Linolenic Acid / Q000737:chemistry / Q000378:metabolism
D004075:Diglycerides; D005227:Fatty Acids; D010938:Plant Oils; D010940:Plant Proteins; D020033:Protein Isoforms; D014280:Triglycerides; D017962:alpha-Linolenic Acid; D019301:Oleic Acid; D019787:Linoleic Acid