A comparative metabolomic study of NHR-49 in Caenorhabditis elegans and PPAR-alpha in the mouse.
Proton Nuclear Magnetic Resonance spectroscopy and Gas Chromatography Mass Spectrometry based metabolomics has been used in conjunction with multivariate statistics to examine the metabolic changes in Caenorhabditis elegans following the deletion of nuclear hormone receptor-49 (nhr-49). Deletion of the receptor produced profound changes in fatty acid metabolism, in particular an increase in the ratio of unsaturated to saturated fatty acids, a decrease in the concentration of glucose and increases in lactate and alanine. Given the proposed functional similarity between nhr-49 and the mammalian peroxisome proliferator-activated receptors (PPARs) these changes were compared with the metabolome of the PPAR-alpha null mouse. The metabolomic approach demonstrated a number of similarities including the regulation of lipid synthesis, beta-oxidation of fatty acids and changes in glycolysis/gluconeogenesis.
Atherton
Helen J
HJ
The Department of Biochemistry, Tennis Court Road, University of Cambridge, Cambridge CB2 1QW, UK.
Jones
Oliver A H
OA
Malik
Shahid
S
Miska
Eric A
EA
Griffin
Julian L
JL
D000818:Animals; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000235:genetics / Q000502:physiology*; D005227:Fatty Acids / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D005943:Gluconeogenesis / Q000235:genetics; D006019:Glycolysis / Q000235:genetics; D008099:Liver / Q000378:metabolism; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods; D047493:PPAR alpha / Q000235:genetics / Q000502:physiology*; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000502:physiology*
D029742:Caenorhabditis elegans Proteins; D005227:Fatty Acids; C505159:NHR-49 protein, C elegans; D047493:PPAR alpha; D018160:Receptors, Cytoplasmic and Nuclear