Chromosome location and association of haplotypes of insulin-like growth factor binding protein-2 with production performance in swine.
Insulin-like growth factor binding protein-2 is a member of the insulin-like growth factor families. Using a porcine RH panel, the gene was mapped on chromosome 15q22-23. Meanwhile, using polymerase chain reaction single strand conformation polymorphism, genotypic and allelic frequencies were analyzed in 17 pig breeds (total animals 570), together with a chi-square test of Hardy-Weinberg equilibrium. Also the association between haplotypes and production performance was analyzed in a Lantang x Landrace population family (n = 133, total 43 traits). At each locus we investigated, all the breeds showed different genotypic and allelic frequency distributions. In general, the Chinese native pig breeds carried a higher allele A frequency (over 50%) than the European pigs. For production performance, pigs with the CAG haplotype had higher fore-body and rear-body weight than those with the TGT and TAG haplotypes (P < 0.05). Also, pigs with the CAG haplotype had higher bone weight of the rear-body than those with the CAT haplotype (P < 0.05); pigs with the TGT and CAG haplotypes had higher forelimb and rearlimb weight than those with the CAT haplotype (P < 0.01 and P < 0.05, respectively); pigs with the TGG haplotype had higher leaf fat weight than those with the TGT and CAG haplotypes (P < 0.05); and pigs with the CAG haplotype had more stomach weight than those with the CAT and CGT haplotypes (P < 0.01); pigs with the TGT and CAG haplotypes had more ribs and longer body than those with the CGT-TGG, and CAT-TAG haplotypes (P < 0.05). These results suggest that IGFBP-2 is associated with production performance, but our population family was small. More studies with large samples are needed before the IGFBP-2 locus will be useful for a selection program.
Wang
Wenjun
W
College of Food Science, Jiangxi Agricultural University, Nanchang, Jiangxi, 330045, People's Republic of China.
Meng
Qinyong
Q
Hu
Xiaoxiang
X
Fei
Jin
J
Feng
Jidong
J
Liu
Weidong
W
Li
Ning
N
D000818:Animals; D005787:Gene Frequency; D005838:Genotype; D006239:Haplotypes; D018971:Insulin-Like Growth Factor Binding Protein 2 / Q000235:genetics*; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA; D013552:Swine / Q000235:genetics* / Q000254:growth & development*
D018971:Insulin-Like Growth Factor Binding Protein 2