Production of monoclonal antibodies.
This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY).
Yokoyama
Wayne M
WM
Washington University School of Medicine, St. Louis, Missouri.
Christensen
Michelle
M
StemCell Technologies, Inc, Vancouver, British Columbia, Canada.
Santos
Gary Dos
GD
StemCell Technologies, Inc, Vancouver, British Columbia, Canada.
Miller
Diane
D
StemCell Technologies, Inc, Vancouver, British Columbia, Canada.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D000918:Antibody Specificity; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000166:cytology / Q000276:immunology*; D007114:Immunization; D051379:Mice
D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned