title: Single-sperm typing.
abstract: This unit presents protocols for sperm isolation using two different methods, amplification of simple sequence-length polymorphisms (SSLP) and/or single nucleotide polymorphisms (SNP) from single cells or whole genome-amplified single cells using primer extension preamplification (PEP), and discusses the statistical analysis of sperm-typing recombination data. Newer methods for studying recombination over very short distances (a few kilobases) using total sperm DNA and allele-specific PCR are also discussed.
authors.affiliation: Agricultural University of Norway, Aas, Norway.
authors.forename: Sigbjorn
authors.initials: S
authors.lastname: Lien
authors.forename: Joanna
authors.initials: J
authors.lastname: Szyda
authors.forename: Esther P
authors.initials: EP
authors.lastname: Leeflang
authors.forename: Rene
authors.initials: R
authors.lastname: Hubert
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.forename: Karin
authors.initials: K
authors.lastname: Schmitt
authors.forename: Norman
authors.initials: N
authors.lastname: Arnheim
mesh_terms: D002469:Cell Separation; D005434:Flow Cytometry; D005819:Genetic Markers; D005821:Genetic Techniques; D005826:Genetics, Medical; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012685:Sepharose; D017422:Sequence Analysis, DNA / Q000379:methods; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism*
chemical_list: D005819:Genetic Markers; D012685:Sepharose