Kinetic assay methods.
The purpose of this unit is to provide a brief review of issues important in the design of initial-rate assay methods. General aspects of kinetic assay design are discussed, including enzyme and substrate purity, concentration and stability. Also covered are issues such as continuous versus stop-time assay formats, coupled enzyme assays, binding studies, and presentation of initial-rate data.
Allison
R D
RD
University of Florida, Gainesville, Florida, USA.
D002384:Catalysis; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D011485:Protein Binding; D013379:Substrate Specificity
D004798:Enzymes