Identification of potential markers of oocyte competence expressed in bovine cumulus cells matured with follicle-stimulating hormone and/or phorbol myristate acetate in vitro.
Oocyte competence is the ability of the oocyte to complete maturation, undergo successful fertilization, and reach the blastocyst stage. Cumulus cells are indispensable for this process. Their removal significantly affects the blastocyst rates. Moreover, the properties and functions of cumulus cells are regulated by the oocyte. They also reflect the oocyte's degree of maturation. Our study was aimed at identifying markers of oocyte competence that are expressed in bovine cumulus cells. In a previous study in our laboratory, the blastocyst yield following FSH or phorbol myristate acetate (PMA) treatment was 45%%. Therefore, we tested four sets of conditions during the first 6 h of in vitro maturation (IVM): FSH (0.1 microg/ml), PMA (0.1 microM), FSH ++ PMA, and negative control. Extracts from each IVM treatment were hybridized against the same negative control on a microarray containing a partial library of differentially expressed transcripts in the cumulus of competent oocytes collected at 6 h after LH in vivo. Common positive clones between diffrentially treated cells were selected, and 15 candidates were validated by real-time PCR. Based on this, the main candidates expressed in cumulus cells and that could be valuable and indirect markers of oocyte competence are hyaluronan synthase 2 (HAS2), inhibin betaA (INHBA), epidermal growth factor receptor (EGFR), gremlin 1 (GREM1), betacellulin (BTC), CD44, tumor necrosis factor-induced protein 6 (TNFAIP6), and prostaglandin-endoperoxide synthase 2 (PTGS2). These biomarkers could be potential candidates to predict oocyte competence and to select higher-quality embryos for transfer. Additionally, these indirect predictors of oocyte competence and follicular health could improve our knowledge of gene expression patterns in the cumulus and yield insights into the molecular pathways controlling oocyte competence.
Assidi
Mourad
M
Centre de Recherche en Biologie de la Reproduction, Université Laval, Québec, Québec, Canada G1K 7P4.
Dufort
Isabelle
I
Ali
Atef
A
Hamel
Mélanie
M
Algriany
Omran
O
Dielemann
Steph
S
Sirard
Marc-André
MA
D000465:Algorithms; D000818:Animals; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D002417:Cattle / Q000235:genetics* / Q000378:metabolism; D016000:Cluster Analysis; D054885:Cumulus Cells / Q000187:drug effects / Q000378:metabolism*; D004338:Drug Combinations; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage / Q000494:pharmacology*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D009866:Oogenesis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000008:administration & dosage / Q000494:pharmacology*
D015415:Biomarkers; D004338:Drug Combinations; D012333:RNA, Messenger; D005640:Follicle Stimulating Hormone; D013755:Tetradecanoylphorbol Acetate