[Construction and immunological evaluation of recombinant adenovirus containing codon-modified HPV 16 L1 gene].
OBJECTIVE
To construct recombinant adenovirus containing codon-modified HPV16L1 gene, and evaluate systemic and mucosal immunological responses induced after immunization with the recombinant virus.


METHODS
The recombinant adenovirus rAd-mod.HPV16L1 was constructed by Admax kit. The C57 BL/6 mice were immunized by purified rAd-mod.HPV16L1 through different inoculation routes. The immunological effect was evaluated by testing the specific neutralizing antibodies in sera and vaginal secretions of immunized mice through indirect ELISA and neutralization assay based HPV pseudovirus.


RESULTS
The result showed that intramuscular immunization could induce good systemic immunity, but the mucosal immunity was too weak, and immunization via intranasal route could induce satisfactory immunity both in sera and vaginal secretions, while intravaginal immunization failed to induce any specific immunological responses either in sera or vaginal secretions.


CONCLUSIONS
The recombinant adenovirus containing codon- modified HPV16L1 gene was successfully constructed. Immunization through intranasal route could induce satisfactory immunity both in sera and vaginal secretions, while intramuscular immunization could only induce high titer of neutralizing antibodies in sera.
Zhou
Yu-bai
YB
College of Life Science and Bio-engineering; Beijing University of Technology, Beijing 100022, China.
Zhou
Ling
L
Li
Ze-lin
ZL
Sheng
Wang
W
Zeng
Yi
Y
D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D000918:Antibody Specificity; D036022:Capsid Proteins / Q000235:genetics* / Q000276:immunology*; D003062:Codon / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics*; D005260:Female; D005818:Genetic Engineering; D052162:Human papillomavirus 16 / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009856:Oncogene Proteins, Viral / Q000235:genetics* / Q000276:immunology*; D014611:Vaccination
D000914:Antibodies, Viral; D036022:Capsid Proteins; D003062:Codon; D004274:DNA, Recombinant; D009856:Oncogene Proteins, Viral; C063317:L1 protein, Human papillomavirus type 16