[Alternatively spliced variants of constitutive androstane receptor in liver of mouse].
To investigate the existence of alternatively spliced variants of constitutive androstane receptor (CAR) in liver of mouse.



The nucleotide from liver of mouse was purified and the CAR cDNA was amplified by PCR. The fragments of CAR cDNA were cloned to T vector and sequence analysis was performed.



Various spliced variants of CAR in liver mouse were confirmed by DNA sequencing.



There are alternatively spliced variants in CAR, which are located in the ligand binding sequence of CAR.
Sun
Hong-Ying
HY
Institute of Pharmacology, Toxicology and Biopahrm, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Chen
Jing
J
Jiang
Kai
K
Chen
Shu-Qing
SQ
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D000090702:Constitutive Androstane Receptor; D018076:DNA, Complementary / Q000235:genetics; D008099:Liver / Q000378:metabolism*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D022821:RNA Splice Sites; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics*
D000090702:Constitutive Androstane Receptor; D018076:DNA, Complementary; D022821:RNA Splice Sites; D018160:Receptors, Cytoplasmic and Nuclear