title: Protein crystallization in a 100 nl solution with new stirring equipment.
abstract: To investigate quantitatively the effects of stirring on protein crystallization, a new stirring system which can agitate a protein solution, approximately 100 nl, by providing Hagen-Poiseuille flow has been successfully developed. In addition, this new stirring system provides flow with a well defined pattern and velocity. Using this system, hen egg-white lysozyme was crystallized in 100-200 nl solutions while being stirred. The optimum stirring conditions for lysozyme crystals have been explored by evaluating the Reynolds (Re) number and the crystals obtained. Intermittent flow, as well as a low Re number, was found to contribute significantly to the growth of a smaller number of larger crystals.
authors.affiliation: Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Maki
authors.forename: R
authors.initials: R
authors.lastname: Murai
authors.forename: H Y
authors.initials: HY
authors.lastname: Yoshikawa
authors.forename: T
authors.initials: T
authors.lastname: Kitatani
authors.forename: S
authors.initials: S
authors.lastname: Nakata
authors.forename: H
authors.initials: H
authors.lastname: Kawahara
authors.forename: H
authors.initials: H
authors.lastname: Hasenaka
authors.forename: A
authors.initials: A
authors.lastname: Kobayashi
authors.forename: S
authors.initials: S
authors.lastname: Okada
authors.forename: S
authors.initials: S
authors.lastname: Sugiyama
authors.forename: H
authors.initials: H
authors.lastname: Adachi
authors.forename: H
authors.initials: H
authors.lastname: Matsumura
authors.forename: K
authors.initials: K
authors.lastname: Takano
authors.forename: S
authors.initials: S
authors.lastname: Murakami
authors.forename: T
authors.initials: T
authors.lastname: Inoue
authors.forename: T
authors.initials: T
authors.lastname: Sasaki
authors.forename: Y
authors.initials: Y
authors.lastname: Mori
mesh_terms: D003460:Crystallization; D009113:Muramidase / Q000737:chemistry*; D012996:Solutions
chemical_list: D012996:Solutions; D009113:Muramidase