title: Using the Structure-function Linkage Database to characterize functional domains in enzymes.
abstract: The Structure-Function Linkage Database (SFLD; http://sfld.rbvi.ucsf.edu/) is a Web-accessible database designed to link enzyme sequence, structure, and functional information. This unit describes the protocols by which a user may query the database to predict the function of newly sequenced enzymes and to correct misannotated functional assignments for enzymes currently in public databases. It is especially useful in helping a user discriminate functional capabilities of a sequence that is only distantly related to characterized sequences in publicly available databases.
authors.affiliation: University of California, San Francisco, San Francisco, California, USA.
authors.forename: Shoshana
authors.initials: S
authors.lastname: Brown
authors.forename: Patricia
authors.initials: P
authors.lastname: Babbitt
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D003628:Database Management Systems; D030562:Databases, Protein; D004789:Enzyme Activation; D004798:Enzymes / Q000737:chemistry* / Q000145:classification / Q000648:ultrastructure*; D016247:Information Storage and Retrieval / Q000379:methods*; D008969:Molecular Sequence Data; D011485:Protein Binding; D014584:User-Computer Interface
chemical_list: D004798:Enzymes