title: Nucleoside-5'-phosphoimidazolides: reagents for facile synthesis of dinucleoside pyrophosphates.
abstract: A facile method is presented for preparation of dinucleoside pyrophosphate derivatives based on reaction of a nucleoside 5'-monophosphate with carbonyldiimidazole followed by treatment of the resulting nucleoside 5'-phosphoimidazolide with a nucleoside 5'-phosphate. This method is suitable for preparation of pyrophosphates analogous to NAD, FAD, and related natural pyrophosphates. The resulting compounds are useful for mechanistic studies of enzymes that use natural pyrophosphates as co-factors or substrates, and in development of inhibitors that have potential applications as therapeutic agents.
authors.affiliation: Center for Drug Design, The University of Minnesota, Minneapolis, Minnesota, USA.
authors.forename: Liqiang
authors.initials: L
authors.lastname: Chen
authors.forename: Dominik
authors.initials: D
authors.lastname: Rejman
authors.forename: Laurent
authors.initials: L
authors.lastname: Bonnac
authors.forename: Krzysztof W
authors.initials: KW
authors.lastname: Pankiewicz
authors.forename: Steven E
authors.initials: SE
authors.lastname: Patterson
mesh_terms: D002851:Chromatography, High Pressure Liquid; D011756:Diphosphates / Q000138:chemical synthesis*; D007093:Imidazoles / Q000737:chemistry*; D007202:Indicators and Reagents / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009705:Nucleosides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011756:Diphosphates; D007093:Imidazoles; D007202:Indicators and Reagents; D009705:Nucleosides