Diagnosis and treatment of mycoplasma-contaminated cell cultures.
Mycoplasma contamination is a serious and frequent problem in the culture laboratory. Although mycoplasma contamination may be suspected by the failure of cells to thrive, the formal diagnosis rests on the detection of adenosine phosphorylase secretion by infected cell lines. This appendix describes how to test for mycoplasma contamination, and also presents methods for antibiotic treatment of infected cultures.
Fitch
F W
FW
University of Chicago, Chicago, Illinois, USA.
Gajewski
T F
TF
Yokoyama
W M
WM
D018929:Cell Culture Techniques; D003584:Cytological Techniques / Q000379:methods*; D009174:Mycoplasma / Q000201:enzymology / Q000302:isolation & purification*; D009175:Mycoplasma Infections / Q000382:microbiology*; D011683:Purine-Nucleoside Phosphorylase / Q000032:analysis
C019603:adenosine phosphorylase; D011683:Purine-Nucleoside Phosphorylase