Differentiation and characterization of T cells.
This paper outlines current standard methods of inducing T lymphopoiesis in vitro and in vivo. Reference is made to both murine and human systems. In addition to differentiation assays, methods to optimally characterize output cells are discussed. In bringing together a number of existing protocols, many techniques important in investigating T cell development can be reviewed in one place.
Robertson
Paul
P
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
Scadden
David T
DT
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.
D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D002454:Cell Differentiation; D019070:Cell Lineage; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques / Q000379:methods; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology; D013950:Thymus Gland / Q000166:cytology
D018952:Antigens, CD34