title: Chromosome microdissection.
abstract: This unit describes chromosome microdissection methodologies that are useful in both clinical and research laboratories. In combination with FISH, region-specific probes can be made to determine the origin of marker chromosomes. Another application of the microdissection technology is in the generation of regionspecific cDNA libraries, known as microdissection-mediated cDNA capture.
authors.affiliation: National Institute for Human Genome Research, Bethesda, Maryland, USA.
authors.forename: P
authors.initials: P
authors.lastname: Meltzer
authors.forename: M
authors.initials: M
authors.lastname: Bittner
mesh_terms: D002877:Chromosomes, Human / Q000235:genetics* / Q000648:ultrastructure; D005826:Genetics, Medical; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008677:Metaphase; D042282:Microdissection / Q000379:methods*; D016133:Polymerase Chain Reaction