title: Detection of mutations by denaturing gradient gel electrophoresis.
abstract: This unit describes the procedure for determining the melting profile for a given PCR-amplified sequence by perpendicular denaturing gradient gel electrophoresis (DGGE) and, using that information, for developing a screening assay based on either parallel DGGE, CDGE (Constant Denaturant Gel Electrophoresis), or TTGE (Temporal/Temperature Gradient Electrophoresis). Four support protocols describe techniques for pouring perpendicular and parallel denaturing gradient gels, constant denaturant gels, and temporal temperature gradient gels.
authors.affiliation: The Norwegian Radium Hospital, Oslo, Norway.
authors.forename: A L
authors.initials: AL
authors.lastname: Børresen-Dale
authors.forename: E
authors.initials: E
authors.lastname: Hovig
authors.forename: B
authors.initials: B
authors.lastname: Smith-Sørensen
mesh_terms: D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004252:DNA Mutational Analysis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005826:Genetics, Medical; D006801:Humans; D009691:Nucleic Acid Denaturation
chemical_list: D004247:DNA