Assaying proteases in cellular environments.
To better study protease activation, regulation, and inhibition physiologically or pharmacologically, it is often advantageous to use cell-based systems. The introduction of the cellular environment poses additional challenges for the assay of the protease of interest. In this unit, various methods for monitoring protease activity in cell-based systems is discussed. The scope of the unit is restricted to mammalian cell system. It is also stressed that specific considerations or requirements for a particular protease or peptidase are often needed in order to optimize the assay.
Wang
Kevin K W
KK
Pfizer Global Research and Development, Ann Arbor, Michigan, USA.
D000818:Animals; D002477:Cells / Q000201:enzymology; D004789:Enzyme Activation; D006801:Humans; D008722:Methods; D010447:Peptide Hydrolases / Q000032:analysis* / Q000378:metabolism*; D011480:Protease Inhibitors
D011480:Protease Inhibitors; D010447:Peptide Hydrolases