title: Expression of avian beta-defensins in the oviduct and effects of lipopolysaccharide on their expression in the vagina of hens.
abstract: The aims of this study were to (i) determine the types of avian beta-defensin genes (AvbetaD) expressed in the hen oviduct and (ii) to examine the effects of lipopolysaccharide (LPS) treatment in vivo on their expression in the vagina. Birds were i.v. treated with LPS (1 mg/kg of BW), and subsequently the oviducts were analyzed 0, 3, 6, 12, or 24 h after LPS administration. The mRNA expression for AvbetaD was examined by reverse transcription-PCR using RNA preparations from the mucosal tissues of all the oviductal segments. Furthermore, changes in their mRNA expression profiles in the vagina were analyzed by semiquantitative reverse transcription-PCR. The AvbetaD-1, -2, -3, -4, -5, -7, -8, -9, -10, -11, and -12 were identified in each oviductal segment from infundibulum to vagina. Among these AvbetaD, the expression of AvbetaD-3, -5, -10, -11, and -12 in the vagina were significantly increased in response to LPS treatment, whereas the others did not show significant changes. These results suggest that all 11 types of AvbetaD are expressed in the hen oviduct and at least 5 of them in the vagina show increased expression in response to LPS.
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
authors.forename: A M Abdel
authors.initials: AM
authors.lastname: Mageed
authors.forename: N
authors.initials: N
authors.lastname: Isobe
authors.forename: Y
authors.initials: Y
authors.lastname: Yoshimura
mesh_terms: D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology*; D010057:Oviducts / Q000187:drug effects / Q000378:metabolism*; D014621:Vagina / Q000378:metabolism*; D023083:beta-Defensins / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D023083:beta-Defensins