Direct selection of cDNAs using genomic contigs.
In the , uncloned cDNA or cDNA from a library with highly repetitive sequences suppressed are hybridized to biotinylated genomic DNAs. The selected sequences are amplified by PCR and rehybridized to create a secondary-selected enriched cDNA library. Biotinylation of cloned genomic DNA is presented in the first support protocol. Linker addition and suppression of repeats in uncloned DNA is presented in the second support protocol and insert amplification and suppression of repeats from libraries is detailed in the third support protocol. In the , uncloned cDNA or cDNA from a library with highly repetitive sequences suppressed are hybridized to biot.
Lovett
M
M
University of Texas Southwestern Medical Center at Dallas, Dallas, Texas.
D000818:Animals; D019921:Biotinylation; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D020451:Contig Mapping / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics* / Q000302:isolation & purification; D005826:Genetics, Medical; D015698:Genomic Library; D006801:Humans; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid
D018076:DNA, Complementary