Heat shock proteins HSP70 and HSP27 in the cerebral spinal fluid of patients undergoing thoracic aneurysm repair correlate with the probability of postoperative paralysis.
An understanding of the time course and correlation with injury of heat shock proteins (HSPs) released during brain and/or spinal cord cellular stress (ischemia) is critical in understanding the role of the HSPs in cellular survival, and may provide a clinically useful biomarker of severe cellular stress. We have analyzed the levels of HSPs in the cerebrospinal fluid (CSF) from patients who are undergoing thoracic aneurysm repair. Blood and CSF samples were collected at regular intervals, and CSF was analyzed by enzyme-linked immunosorbent assay for HSP70 and HSP27. These results were correlated with intraoperative somatosensory-evoked potentials measurements and postoperative paralysis. We find that the levels of these proteins in many patients are elevated and that the degree of elevation correlates with the risk of permanent paralysis. We hypothesize that sequential measurement intraoperatively of the levels of the heat shock proteins HSP70 and HSP27 in the CSF can predict those patients who are at greatest risk for paralysis during thoracic aneurysm surgery and will allow us to develop means of preventing or attenuating this severe and often fatal complication.
Hecker
James G
JG
Department of Anesthesia, University of Pennsylvania, Philadelphia, PA 19104-6112, USA. heckerj@uphs.upenn.edu
Sundram
Hari
H
Zou
Shaomin
S
Praestgaard
Amy
A
Bavaria
Joseph E
JE
Ramchandren
Sindhu
S
McGarvey
Michael
M
D000368:Aged; D017545:Aortic Aneurysm, Thoracic / Q000134:cerebrospinal fluid* / Q000150:complications / Q000601:surgery*; D013504:Cardiovascular Surgical Procedures / Q000009:adverse effects; D016001:Confidence Intervals; D003710:Demography; D005260:Female; D055551:HSP27 Heat-Shock Proteins / Q000134:cerebrospinal fluid*; D018840:HSP70 Heat-Shock Proteins / Q000134:cerebrospinal fluid*; D006360:Heat-Shock Proteins; D006801:Humans; D008297:Male; D008875:Middle Aged; D018832:Molecular Chaperones; D015999:Multivariate Analysis; D010243:Paralysis / Q000134:cerebrospinal fluid / Q000150:complications / Q000209:etiology*; D011183:Postoperative Complications / Q000134:cerebrospinal fluid / Q000209:etiology*; D012044:Regression Analysis; D014945:Wound Healing
D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones