Serum protein expression profiling in pediatric Hodgkin lymphoma: a report from the Children's Oncology Group.
BACKGROUND
The prognosis for children with Hodgkin lymphoma (HL) treated with a risk adjusted combination of radiation therapy and multi-drug chemotherapy has markedly improved. There remains a group of patients whose disease either recurs or does not respond to therapy. Protein expression profiling has been used to define protein characteristics of serum from adult patients in order to improve screening for early diagnosis. However, profiling for the purpose of staging and defining prognostic characteristics of childhood diseases is not well studied. The current stage-based risk assignment of HL cannot predict the patients within a risk group that are destined to recur or do not respond to therapy. Thus, a need exists to develop new methodologies to better stratify the risk classification of pediatric HL.


METHODS
We have completed a preliminary project to identify characteristic serum protein peaks determined by protein expression profiling in serum of 22 subjects with HL, 13 with stage II HL and 9 with stage III or IV.


RESULTS
Protein profiling successfully discriminated between high grade (III/IV) HL and low grade (II) HL.


CONCLUSIONS
These data lay the basis for prospective studies to identify protein expression profiles useful for diagnosis, prognosis, treatment stratification, and the follow-up of minimal residual disease.
Qi
Lining
L
Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, Virginia, USA.
Cazares
Lisa
L
Johnson
Cris
C
de Alarcon
Pedro
P
Kupfer
Gary M
GM
Semmes
O John
OJ
D000293:Adolescent; D001798:Blood Proteins / Q000032:analysis*; D002648:Child; D005260:Female; D006689:Hodgkin Disease / Q000097:blood* / Q000473:pathology; D006801:Humans; D008297:Male; D009367:Neoplasm Staging; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D000515:alpha 1-Antitrypsin / Q000032:analysis
D001798:Blood Proteins; D000515:alpha 1-Antitrypsin