title: Enzymatic labeling of DNA.
abstract: This appendix describes the preparation of uniformly labeled radioactive probes by nick translation and random oligonucleotide-primed synthesis, as well as the end-labeling of oligonucleotides by T4 polynucleotide kinase. Accompanying these protocols are methods for removing unincorporated dNTP precursors with spin columns and for measuring the specific activity of a probe by acid precipitation.
authors.affiliation: Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: S
authors.initials: S
authors.lastname: Tabor
authors.forename: K
authors.initials: K
authors.lastname: Struhl
mesh_terms: D011232:Chemical Precipitation; D004247:DNA / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D005826:Genetics, Medical; D006801:Humans; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes; D010761:Phosphorus Radioisotopes; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D012313:RNA / Q000737:chemistry / Q000235:genetics
chemical_list: D015345:Oligonucleotide Probes; D010761:Phosphorus Radioisotopes; D012313:RNA; D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase