Nucleobase-caged phosphoramidites for oligonucleotide synthesis.
Caged compounds are biologically active agents bearing a photolabile group in a strategic position, which makes them temporarily inactive. These compounds can then be delivered to a biological sample without immediately generating an effect. When the sample is then irradiated, e.g., with a laser in a (confocal) microscope, the activity of the substance is released with exact spatiotemporal and dose control. This unit deals with the synthesis of protected nucleoside phosphoramidites bearing a caging group on the nucleobase, which prevents the nucleobases from forming normal Watson-Crick base pairs. These amidites can be used to generate caged oligodeoxynucleotides with a transitory local perturbation that adds an element of spatiotemporal control to oligonucleotide-based applications.
Heckel
Alexander
A
University of Bonn, Bonn, Germany.
D000577:Amides / Q000737:chemistry*; D002855:Chromatography, Thin Layer; D009682:Magnetic Resonance Spectroscopy; D018613:Microscopy, Confocal; D009841:Oligonucleotides / Q000138:chemical synthesis*; D010756:Phosphoric Acids / Q000737:chemistry*
D000577:Amides; D009841:Oligonucleotides; D010756:Phosphoric Acids; C011067:phosphoramidic acid