[The role of cell pathway in apoptosis of fibroblasts from proliferative scar induced by steroid or IFN].
OBJECTIVE
This paper is to investigate the effects of steroid or IFN alpha-2b on apoptosis and cell pathway of fibroblasts from keloids, hypertrophic scars and normal skins and different responses of different fibroblasts.


METHODS
6 samples from keloid, hypertrophic scar and normal skin were collected respectively and fibroblasts from different sources were cultured in vitro. After different fibroblasts were treated with dexamethasone (0.1 mg/ml) or IFN alpha-2b (1000 U/ml), Bax and Bcl-2 protein expressions were detected in situ by immunohistochemical staining; DNA ladders of different fibroblasts were observed by gel electrophoresis; and relative activated (phospho-) ERK1/2 and JNK pathways were detected by method of FACE ELISA.


RESULTS
Dexamethasone could induce apoptosis of fibroblasts from keloids, hypertrophic scars and normal skins through activating (phospho-) ERK1/2 and JNK pathways; IFN alpha-2b could not induce apoptosis of fibroblasts from different sources. IFN alpha-2b could inhibit (phospho-) ERK1/2 pathway and could not affect (phospho-) JNK pathways of fibroblasts from keloid and hypertrophic scar. IFN alpha-2b could affect neither (phospho-) ERK1/2 pathway nor (phospho-) JNK pathways of fibroblasts from normal skin.


CONCLUSIONS
The responses of different fibroblasts to steroid or IFN alpha-2b were different.
Xu
Shao-Jun
SJ
Department of Burn and Plastic Surgery, Hangzhou Second Hospital, Hangzhou 310015, China.
Teng
Jian-Ying
JY
Xie
Jing
J
Wu
Ai-Jing
AJ
Chen
Dong-Ming
DM
Bao
Wei-Han
WH
D000328:Adult; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D006728:Hormones / Q000494:pharmacology; D006801:Humans; D000077190:Interferon alpha-2; D016898:Interferon-alpha / Q000494:pharmacology; D007627:Keloid / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D011994:Recombinant Proteins; D015398:Signal Transduction
D006728:Hormones; D000077190:Interferon alpha-2; D016898:Interferon-alpha; D011994:Recombinant Proteins