title: Effects of short-term high-fat, high-energy diet on hepatic and myocardial triglyceride content in healthy men.
abstract: BACKGROUND
  An association has been suggested between elevated plasma nonesterified fatty acid (NEFA) levels, myocardial triglyceride (TG) accumulation, and myocardial function.
  
  
  OBJECTIVE
  Our objective was to investigate the effects of an elevation of plasma NEFA by a high-fat, high-energy (HFHE) diet on hepatic and myocardial TG accumulation, and on myocardial function.
  
  
  METHODS
  There were 15 healthy males (mean +/- sd age: 25.0 +/- 6.6 yr) subjected to a 3-d HFHE diet consisting of their regular diet, supplemented with 800 ml cream (280 g fat) every day.
  
  
  METHODS
  (1)H-magnetic resonance spectroscopy was performed for assessing hepatic and myocardial TGs. Furthermore, left ventricular function was assessed using magnetic resonance imaging.
  
  
  RESULTS
  The HFHE diet increased hepatic TGs compared with baseline (from 2.01 +/- 1.79 to 4.26 +/- 2.78%; P = 0.001) in parallel to plasma TGs and NEFA. Myocardial TGs did not change (0.38 +/- 0.18 vs. 0.40 +/- 0.12%; P = 0.7). The HFHE diet did not change myocardial systolic function. Diastolic function, assessed by dividing the maximum flow across the mitral valve of the early diastolic filling phase by the maximum flow of the atrial contraction (E/A ratio), decreased compared with baseline (from 2.11 +/- 0.39 to 1.89 +/- 0.33; P = 0.031). This difference was no longer significant after adjustment for heart rate (P = 0.12).
  
  
  CONCLUSIONS
  Short-term HFHE diet in healthy males results in major increases in plasma TG and NEFA concentrations and hepatic TGs, whereas it does not influence myocardial TGs or myocardial function. These observations indicate differential, tissue-specific partitioning of TGs and/or fatty acids among nonadipose organs during HFHE diet.
authors.affiliation: Department of Radiology, Leiden University Medical Center, Leiden, The Netherlands. R.W.van_der_meer@lumc.nl
authors.forename: Rutger W
authors.initials: RW
authors.lastname: van der Meer
authors.forename: Sebastiaan
authors.initials: S
authors.lastname: Hammer
authors.forename: Hildo J
authors.initials: HJ
authors.lastname: Lamb
authors.forename: Marijke
authors.initials: M
authors.lastname: Frölich
authors.forename: Michaela
authors.initials: M
authors.lastname: Diamant
authors.forename: Luuk J
authors.initials: LJ
authors.lastname: Rijzewijk
authors.forename: Albert
authors.initials: A
authors.lastname: de Roos
authors.forename: Johannes A
authors.initials: JA
authors.lastname: Romijn
authors.forename: Johannes W A
authors.initials: JW
authors.lastname: Smit
mesh_terms: D000328:Adult; D004041:Dietary Fats / Q000008:administration & dosage*; D002149:Energy Intake; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D006801:Humans; D008099:Liver / Q000378:metabolism*; D008279:Magnetic Resonance Imaging; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D009206:Myocardium / Q000378:metabolism*; D014280:Triglycerides / Q000032:analysis / Q000378:metabolism*; D016277:Ventricular Function, Left
chemical_list: D004041:Dietary Fats; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides