Highly efficient Agrobacterium-mediated transformation of Volvariella volvacea.
Agrobacterium tumefaciens-mediated transformation (ATMT) was successfully applied to the edible straw mushroom, Volvariella volvacea. Mycelium pellets were transformed to cold stress resistance using the afp gene as both a selective marker and a reporter gene, under the control of a heterologous Lentinula edodes gpd promoter. The efficiency of transformation is over 100 times higher than that previously reported in V. volvacea. Stable integration of the afp gene with 1-4 copy numbers was confirmed in all 10 randomly selected transgenic events by Southern blot analysis. The mitotic stability of the transformants was demonstrated after five successive transfers on PDA medium without selection pressure and the PCR analysis of basidiospores harvested from transformants.
Wang
Jie
J
Department of Bioengineering, College of Food Science, South China Agricultural University, 482 Wushan Street, Tianhe, Guangdong 510640, China.
Guo
Liqiong
L
Zhang
Kai
K
Wu
Qi
Q
Lin
Junfang
J
D000363:Agaricales / Q000235:genetics*; D015139:Blotting, Southern; D005798:Genes, Bacterial; D005822:Genetic Vectors; D042822:Genomic Instability; D016133:Polymerase Chain Reaction; D012231:Rhizobium / Q000235:genetics*; D014169:Transformation, Bacterial