Enhancer analysis by chicken embryo electroporation with aid of genome comparison.
The identification of the enhancers associated with each developmentally regulated gene is a first step to clarify the regulatory mechanisms underlying embryogenesis. The electroporation technique using chicken embryo is a powerful tool to identify such enhancers. The technique enables us to survey a large genomic region and to analyze the enhancers in great detail. Comparison of the genomic sequences of the chicken and other vertebrate species identifies conserved non-coding sequence blocks to which the functionally identified enhancers often correspond. In this review, I describe in detail the methods to analyze the enhancers using the chicken embryo electroporation and genome comparison.
Uchikawa
Masanori
M
Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan. uchikawa@fbs.osaka-u.ac.jp
D000818:Animals; D002642:Chick Embryo; D015509:Developmental Biology / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000378:metabolism; D004742:Enhancer Elements, Genetic; D005091:Exons; D018507:Gene Expression Regulation, Developmental; D005821:Genetic Techniques; D016678:Genome; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008957:Models, Genetic
D049452:Green Fluorescent Proteins