An in silico analysis of cytochrome c from Phanerochaete chrysosporium: its amino acid sequence and characterization of gene structural elements.
An in silico approach was used to investigate cytochrome c and the cytochrome c gene of Phanerochaete chrysosporium. The cytochrome c gene contains four introns. Omission of the introns reveals a DNA sequence coding for a complete predicted amino acid sequence for P. chrysosporium cytochrome c consistent with those of other cytochromes c. Fungal cytochromes c often have a short N-terminal peptide preceding a Gly that is the N-terminal amino acid in many cytochromes c. Thus a microexon codes for an N-terminal pentapeptide (MetProTyrAlaPro) in P. chrysosporium that is identical to the N-terminal pentapeptide of Schizosaccharomyces pombe, a well studied yeast, the genome of which bears more similarity to higher eukaryotes than to other fungi. The fourth intron, when omitted, reveals the presence of another microexon resulting in a sequence for the C-terminal portion of the protein and the stop codon. Interestingly, two interpretations for the sequence of this intron leads to predictions that the C-terminal sequence ends with either AlaValAsn or AlaTyr. Selected aspects of the molecular architecture of cytochrome c and regulatory control elements of the P. chrysosporium cytochrome c gene were analyzed and compared to those present in other fungi and to those present in genes for lignin peroxidases and cytochromes P-450, two important families of hemeproteins produced by this fungus.
Bumpus
John A
JA
Department of Chemistry and Biochemistry, University of Northern Iowa, Cedar Falls, Iowa 50614, USA. john.bumpus@uni.edu
Trax
Mark
M
Reisdorph
Ashley
A
Boyd
Cindi
C
Gilbert
Damien
D
Techau
Sara
S
Ventullo
Roy M
RM
D000595:Amino Acid Sequence; D001483:Base Sequence; D019295:Computational Biology; D045304:Cytochromes c / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D016681:Genome, Fungal; D007438:Introns; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000235:genetics*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid
D045304:Cytochromes c