title: Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity.
abstract: Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescens Pf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.
authors.affiliation: Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801, USA.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zha
authors.forename: Sheryl B
authors.initials: SB
authors.lastname: Rubin-Pitel
authors.forename: Huimin
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D002384:Catalysis; D030562:Databases, Protein; D019020:Directed Molecular Evolution; D014644:Genetic Variation / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D015202:Protein Engineering; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000235:genetics*
chemical_list: D000217:Acyltransferases; C020141:flavanone synthetase