title: In vitro evaluation of experimental agents for anti-HIV activity.
abstract: This unit presents an assay that has proven useful as an initial screening test is an HIV cytopathic effect (CPE) inhibition assay in which immortalized T cell lines (e.g., ATH8 or MT2) that are profoundly sensitive to the cytopathic effect of certain strains of HIV are utilized as target cells. Additional protocols assess the anti-HIV activity of certain candidate agents by measuring inhibition of syncytium formation or p24 gag protein production by ELISA. Calculation of the 50% inhibitory concentration (IC(50)) is also presented.
authors.affiliation: University of California, San Diego, La Jolla, California, USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Richman
authors.forename: V A
authors.initials: VA
authors.lastname: Johnson
authors.forename: D M
authors.initials: DM
authors.lastname: Mayers
authors.forename: T
authors.initials: T
authors.lastname: Shirasaka
authors.forename: M C
authors.initials: MC
authors.lastname: O'Brien
authors.forename: H
authors.initials: H
authors.lastname: Mitsuya
mesh_terms: D019380:Anti-HIV Agents / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000821:virology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006678:HIV / Q000502:physiology*; D006801:Humans; D007158:Immunologic Techniques
chemical_list: D019380:Anti-HIV Agents