Measuring protein thermostability by differential scanning calorimetry.
Differential scanning calorimetry (DSC) the method of choice to study the conformational stability of biological macromolecules and proteins in particular. This unit presents step-by-step protocols for DSC, including sample preparation and interpretation of the results in the simplest cases as well as calibration of the apparatus and maintenance of DSC cells. Various general experimental considerations, including possible pitfalls and errors, are also discussed.
Makhatadze
G I
GI
Texas Tech University, Lubbock, Texas, USA.
D002152:Calorimetry, Differential Scanning / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D013816:Thermodynamics
D011506:Proteins