title: Primary diffuse large B-cell lymphoma of bone displays preferential rearrangements of the c-MYC or BCL2 gene.
abstract: We selected a series of 63 primary diffuse large B-cell lymphomas (DLBCLs) of bone collected in tissue microarrays from centers in France and Brazil. These cases were classified according to the expression of antigens associated with germinal center (GC; n = 42) or non-GC (n = 21) stages of B-cell differentiation. By fluorescence in situ hybridization, we found a substantial number of cases with a rearrangement of BCL2 (9/32) and c -MYC (n = 3), whereas the PAX5, BCL6, BCL1 cyclin D1, and ALK genes were in germline configuration. It is interesting that 1 case, with a GC phenotype, showed dual BCL2 and c -MYC rearrangement. The majority of the cases with rearrangements were of the GC phenotype. These results, associated with the lack of BCL6 rearrangement, suggest that bone DLBCL represents a specific group within extranodal B-cell lymphomas.
authors.affiliation: Center of Physiopathology of Toulouse-Purpan, and Department of Pathology, Paul-Sabatier University, Toulouse, France.
authors.forename: Francisco Pignataro
authors.initials: FP
authors.lastname: Lima
authors.forename: Marina
authors.initials: M
authors.lastname: Bousquet
authors.forename: Anne
authors.initials: A
authors.lastname: Gomez-Brouchet
authors.forename: Geisilene Russano
authors.initials: GR
authors.lastname: de Paiva
authors.forename: Eliane Maria Ingrid
authors.initials: EM
authors.lastname: Amstalden
authors.forename: Fernando Augusto
authors.initials: FA
authors.lastname: Soares
authors.forename: Nicole
authors.initials: N
authors.lastname: Dastugue
authors.forename: Jose
authors.initials: J
authors.lastname: Vassallo
authors.forename: Pierre
authors.initials: P
authors.lastname: Brousset
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics* / Q000473:pathology; D019254:Genes, bcl-2 / Q000235:genetics*; D016259:Genes, myc / Q000235:genetics*; D018858:Germinal Center / Q000473:pathology; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000473:pathology; D046888:Tissue Array Analysis