Cellular delivery of locked nucleic acids (LNAs).
Locked nucleic acids (LNAs) are RNA derivatives that have an O-methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high-affinity binding to complementary sequences. They are synthesized using standard DNA/RNA synthesis methods, and have a negatively charged backbone that confers good solubility. This unit describes a method for the introduction of LNA oligomers into cells. A support protocol also describes the determination of melting temperatures for LNA oligomers.
Braasch
Dwaine A
DA
University of Texas Southwestern Medical Center at Dallas, USA.
Corey
David R
DR
D002463:Cell Membrane Permeability; D002477:Cells / Q000378:metabolism*; D016503:Drug Delivery Systems; D009841:Oligonucleotides / Q000493:pharmacokinetics*; D012313:RNA; D013696:Temperature
D009841:Oligonucleotides; C477371:locked nucleic acid; D012313:RNA