Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1.
Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.
Moon
Jai-Hee
JH
Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology, Korea University, 5-1, Anam-Dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
Yoon
Byung Sun
BS
Kim
Bona
B
Park
Gyuman
G
Jung
Hye-Youn
HY
Maeng
Isaac
I
Jun
Eun Kyoung
EK
Yoo
Seung Jun
SJ
Kim
Aeree
A
Oh
Sejong
S
Whang
Kwang Youn
KY
Kim
Hyunggee
H
Kim
Dong-Wook
DW
Kim
Ki Dong
KD
You
Seungkwon
S
D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D049109:Cell Proliferation; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D005819:Genetic Markers; D006023:Glycoproteins / Q000235:genetics; D024002:HMGB Proteins / Q000235:genetics; D007381:Intermediate Filament Proteins / Q000235:genetics; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics; D064231:Nestin; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D010455:Peptides / Q000235:genetics; D063150:Polycomb Repressive Complex 1; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors / Q000235:genetics
D000071916:AC133 Antigen; D015703:Antigens, CD; C489421:Bmi1 protein, mouse; C417743:Cdkn2a protein, mouse; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D006023:Glycoproteins; D024002:HMGB Proteins; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D009687:Nuclear Proteins; D010455:Peptides; C000606024:Prom1 protein, mouse; D047428:Protein Kinase Inhibitors; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D014157:Transcription Factors; D063150:Polycomb Repressive Complex 1