title: Effects of aging and dietary antler supplementation on the calcium-regulating hormones and bone status in ovariectomized SAMP8 mice.
abstract: This study was conducted to investigate the effects of aging and long-term dietary antler supplementation on the calcium-regulating hormones and bone status in ovariectomized (Ovx) SAMP8 mice. The female SAMP8 mice were divided into four groups (in each group n = 6), Ovx or sham operated at the age of 2 months, and fed with 0.2% antler containing diet or control diet from the age of 2.5 months. The samples were collected at the age of 3, 6, 9, 12, and 15 months, respectively, for physicochemical analyses, biochemical analyses, and the determination of hormones by radioimmunoassay. The results showed that plasma calcium (Ca) concentrations were maintained in a narrow range in all groups throughout the whole experimental period. With aging and/or ovariectomy, plasma parathyroid hormone (PTH) and 1,25-dihydroxycholecalciferol (1,25-(OH)2-D3) levels increased, and plasma phosphorus (P) and calcitonin (CT) levels decreased, and the femoral bone densities and Ca contents increased during the earlier stage, and then decreased gradually in all groups. Plasma PTH and 1,25-(OH)2-D3 levels in the Ovx mice were significantly higher than those in the intact mice, and plasma P concentrations, plasma CT levels, femoral bone densities, and femoral Ca contents in the Ovx mice were significantly lower than those in the intact mice. In addition, the decreases of plasma P levels, plasma CT levels, femoral bone densities, and femoral Ca contents, and the increases of plasma PTH levels were moderated by antler administration in both Ovx and intact mice. However, there was no effect of the dietary antler supplementation on the plasma 1,25-(OH)2-D3 levels in the female mice. It is concluded that prolonged dietary antler supplementation has important positive effects on bone loss with age and/ or ovarian function deficiency.
authors.affiliation: Department of Sports, Health and Leisure, Yungta Institute of Technology and Commerce, Pingtung, Taiwan, ROC.
authors.forename: Chun-Chi
authors.initials: CC
authors.lastname: Chen
authors.forename: Mei-Hui
authors.initials: MH
authors.lastname: Liu
authors.forename: Ming-Fu
authors.initials: MF
authors.lastname: Wang
authors.forename: Cheng-Chin
authors.initials: CC
authors.lastname: Chen
mesh_terms: D000375:Aging / Q000502:physiology*; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D000999:Antlers / Q000737:chemistry*; D015519:Bone Density / Q000187:drug effects; D050071:Bone Density Conservation Agents / Q000494:pharmacology*; D001842:Bone and Bones / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D002116:Calcitonin / Q000097:blood; D002117:Calcitriol / Q000097:blood; D002118:Calcium / Q000378:metabolism*; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D019587:Dietary Supplements; D005260:Female; D005269:Femur / Q000737:chemistry; D008516:Medicine, Chinese Traditional; D051379:Mice; D008815:Mice, Inbred Strains; D010052:Ovariectomy; D010281:Parathyroid Hormone / Q000097:blood; D010758:Phosphorus / Q000097:blood; D011863:Radioimmunoassay
chemical_list: D050071:Bone Density Conservation Agents; D010281:Parathyroid Hormone; D010758:Phosphorus; D002116:Calcitonin; D000469:Alkaline Phosphatase; D002117:Calcitriol; D002118:Calcium