Particle-mediated gene delivery in vivo and in vitro.
Particle-mediated or "gene gun" technology has been developed as a nonviral method for gene transfer into various mammalian tissues. Gene delivery is achieved by physical force: a strong shock wave is generated that accelerates DNA-coated gold particles to high speeds, providing them with the momentum needed to penetrate the targeted cells. This unit describes general procedures for in vivo and in vitro DNA and RNA transfections by particle-mediated delivery. The Basic Protocol and an alternate protocol address in vivo delivery to mouse skin. In vitro delivery to cryopreserved and adherent cells is also described.
Yang
N S
NS
Auragen, Inc, Middleton, Wisconsin, USA.
Burkholder
J
J
McCabe
D
D
Neumann
V
V
Fuller
D
D
D000818:Animals; D019470:Biolistics / Q000379:methods*; D002448:Cell Adhesion; D002478:Cells, Cultured; D004274:DNA, Recombinant / Q000008:administration & dosage / Q000235:genetics; D005826:Genetics, Medical; D006801:Humans; D066298:In Vitro Techniques; D051379:Mice; D012313:RNA / Q000008:administration & dosage / Q000235:genetics; D012867:Skin; D014162:Transfection
D004274:DNA, Recombinant; C039919:RNA, recombinant; D012313:RNA