title: Reversal effect of Ganoderma lucidum polysaccharide on multidrug resistance in K562/ADM cell line.
abstract: OBJECTIVE
  To investigate the reversal effect of Ganoderma lucidum polysaccharides (Gl-PS) on multidrug resistance (MDR) in the adriamycin (ADM)-resistant leukemic cell line K562/ADM.
  
  
  METHODS
  Cytotoxicity was assayed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide method; the ADM concentration in cells was determined by flow cytometry and confocal laser scanning microscopy techniques; the expression of P-glycoprotein was assayed by flow cytometry; and the mRNA expression levels of MDR-1 and MDR-associated protein (MRP)1 were determined by RT-PCR.
  
  
  RESULTS
  Gl-PS reversed MDR in K562/ADM cells. Gl-PS obviously reversed the resistance of K562/ADM to doxorubicin. The reversing factors of Gl-PS at 10 and 20 mg/L were 6.46 and 6.80, respectively. MDR-1 and MRP1 transcription were downregulated by 10 and 50 mg/L Gl-PS.
  
  
  CONCLUSIONS
  Gl-PS can reverse the MDR by downregulating the expression of MDR-1 and MRP1 in K562/ADM cells.
authors.affiliation: Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100083, China. lwdpharma@126.com
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Li
authors.forename: Bo-di
authors.initials: BD
authors.lastname: Zhang
authors.forename: Ran
authors.initials: R
authors.lastname: Wei
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Liu
authors.forename: Zhi-Bin
authors.initials: ZB
authors.lastname: Lin
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000378:metabolism*; D018432:Drug Resistance, Multiple / Q000187:drug effects* / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D005562:Formazans / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D011134:Polysaccharides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D020940:Reishi / Q000235:genetics / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000378:metabolism
chemical_list: D000903:Antibiotics, Antineoplastic; D005562:Formazans; D011134:Polysaccharides; D012333:RNA, Messenger; D013778:Tetrazolium Salts; C079782:MTT formazan; D004317:Doxorubicin