Diagnostic role of circulating free plasma DNA detection in patients with localized prostate cancer.
To analyze the potential diagnostic relevance of free plasma DNA (FPDNA), we enrolled 64 patients with localized prostate cancer (CaP). FPDNA was quantified by real-time polymerase chain reaction assessment of the HTERT gene in blood samples from 64 patients with CaP and 45 healthy males. Methylation of the GSTP1 gene was used to confirm the neoplastic origin of FPDNA in selected cases. The mean +/- SD levels of FPDNA were higher in patients with CaP (15.4 +/- 10.9 ng/mL) than in control subjects (5.5 +/- 3.5 ng/mL; P <.001). By using the best cutoff value, the sensitivity of the test was 80%, the specificity was 82%, the area under the receiver operating characteristic curve, 0.881. High FPDNA values were significantly associated with pathologic T3 stage (P = . 035). Methylation of the GSTP1 gene was found in 4 (25%) of 16 FPDNA samples and 15 (94%) of 16 tissue samples. Quantification of FPDNA discriminates between patients with CaP and healthy subjects and correlates with pathologic tumor stage. FPDNA is a candidate biomarker for early diagnosis and monitoring of CaP.
Altimari
Annalisa
A
Molecular and Transplantation Pathology Laboratory, F. Addarii Institute of Oncology, University of Bologna, Bologna, Italy.
Grigioni
Antonia D'Errico
AD
Benedettini
Elisa
E
Gabusi
Elena
E
Schiavina
Riccardo
R
Martinelli
Antonio
A
Morselli-Labate
Antonio Maria
AM
Martorana
Giuseppe
G
Grigioni
Walter Franco
WF
Fiorentino
Michelangelo
M
D000368:Aged; D019540:Area Under Curve; D014408:Biomarkers, Tumor / Q000097:blood*; D019175:DNA Methylation; D004273:DNA, Neoplasm / Q000097:blood*; D051549:Glutathione S-Transferase pi / Q000235:genetics; D006801:Humans; D008297:Male; D008875:Middle Aged; D011471:Prostatic Neoplasms / Q000097:blood* / Q000175:diagnosis*; D012372:ROC Curve; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019098:Telomerase / Q000097:blood / Q000235:genetics
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi; C509186:TERT protein, human; D019098:Telomerase