title: Determining the CD spectrum of a protein.
abstract: This unit describes the theory behind circular dichroism (CD) and deals with considerations regarding instrumentation and reagents for CD spectrometry. A protocol is provided that outlines the steps in recording a CD spectrum and two support protocols explain the interpretation of near-UV and far-UV CD spectra.
authors.affiliation: Jozef Stefan Institute, Ljubljana, Slovenia.
authors.forename: Roger
authors.initials: R
authors.lastname: Pain
mesh_terms: D002942:Circular Dichroism / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D011506:Proteins