title: Virulence domain of the RYMV genome-linked viral protein VPg towards rice rymv1-2-mediated resistance.
abstract: Virulent variants of Rice yellow mottle virus (genus Sobemovirus) can emerge on the highly resistant rice cultivars Gigante and Bekarosaka. Non-synonymous mutations responsible for the breakdown of the recessive resistance gene rymv1-2 were located in the VPg after determination of its termini in the polyprotein P2a. The secondary structure of this protein was predicted to include a central alpha-helix. The two major amino acids related to virulence are located on the same side of this helix. The 3D topology and the biochemical properties of virulence mutations both suggested a direct site-to-site interaction between RYMV VPg and rice eIF(iso)4G encoded by rymv1.
authors.affiliation: UMR RPB 186, Institut de Recherche pour le Développement, BP 64501, 34394 Montpellier Cedex 5, France. hebrard@mpl.ird.fr
authors.forename: Eugénie
authors.initials: E
authors.lastname: Hébrard
authors.forename: Agnès
authors.initials: A
authors.lastname: Pinel-Galzi
authors.forename: Denis
authors.initials: D
authors.lastname: Fargette
mesh_terms: D000595:Amino Acid Sequence; D039603:Eukaryotic Initiation Factor-4G / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012275:Oryza; D010935:Plant Diseases / Q000276:immunology / Q000821:virology; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D010942:Plant Viruses / Q000737:chemistry* / Q000472:pathogenicity; D011485:Protein Binding; D017434:Protein Structure, Tertiary / Q000235:genetics*; D012328:RNA Viruses / Q000737:chemistry* / Q000472:pathogenicity; D012261:Ribonucleoproteins / Q000235:genetics* / Q000378:metabolism*; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism*; D014774:Virulence
chemical_list: D039603:Eukaryotic Initiation Factor-4G; D010940:Plant Proteins; D012261:Ribonucleoproteins; D017361:Viral Nonstructural Proteins