title: Differential fine-tuning of cochlear implant material-cell interactions by femtosecond laser microstructuring.
abstract: Cochlear implants (CIs) can restore hearing in deaf patients by electrical stimulation of the auditory nerve. To optimize the electrical stimulation, the number of independent channels must be increased by reduction of connective tissue growth on the electrode surface and selective neuronal cell contact. The femtosecond laser microstructuring of the electrode surfaces was performed to investigate the effect of fibroblast growth on the implant material. A cell culture model system was established to evaluate cell-material interactions on these microstructured CI-electrode materials. Fibroblasts were used as a cell culture model for connective tissue formation, and differentiating neuronal-like cells were employed to represent neuronal cells. For nondestructive microscopic examination of living cells on the structured surfaces, the cells were genetically modified to express green fluorescent protein. To investigate the special interaction between the electrode material and the tissue we used electrode material which is originally used for manufacturing CI for human applications, namely platinum (contact material) and silicone carrier material (LSR 30, HCRP 50). Microstructures of various dimensions (groove width 1-10 microm) were generated by using femtosecond laser ablation. The highest fibroblast growth rate was observed on platinum, but cell growth rates on the silicone carrier material were lower. Microstructuring reduced fibroblast cell growth on platinum significantly. On the microstructured silicone, a trend to lower cell growth rates was observed. In addition, microgrooves on platinum surfaces can direct neurite outgrowth parallel to the grooves. The implications of the results are discussed with respect to the design of a microstructured CI surface.
authors.affiliation: Department of Otolaryngology, Medical University of Hannover, Hannover, Germany. Reich.Uta@mh-hannover.de
authors.forename: Uta
authors.initials: U
authors.lastname: Reich
authors.forename: Peter P
authors.initials: PP
authors.lastname: Mueller
authors.forename: Elena
authors.initials: E
authors.lastname: Fadeeva
authors.forename: Boris N
authors.initials: BN
authors.lastname: Chichkov
authors.forename: Timo
authors.initials: T
authors.lastname: Stoever
authors.forename: Tilman
authors.initials: T
authors.lastname: Fabian
authors.forename: Thomas
authors.initials: T
authors.lastname: Lenarz
authors.forename: Guenter
authors.initials: G
authors.lastname: Reuter
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D003054:Cochlear Implants; D004566:Electrodes; D005347:Fibroblasts / Q000166:cytology*; D007834:Lasers; D051379:Mice; D009474:Neurons / Q000166:cytology*; D010984:Platinum; D051381:Rats; D012828:Silicones
chemical_list: D012828:Silicones; D010984:Platinum