Cellular compartment analysis of temporal activity by fluorescence in situ hybridization (catFISH).
This protocol describes a method (cellular compartment analysis of temporal activity by fluorescent in situ hybridization or catFISH) that uses fluorescent in situ hybridization to immediate-early gene RNAs and confocal microscopy to identify neuronal populations activated at two distinct times. The combination of cellular and temporal resolution makes catFISH a valuable tool for investigating the dynamic interactions of neuronal populations associated with different behaviors or cognitive challenges.
Guzowski
J F
JF
University of Arizona, Tucson, Arizona, USA.
Worley
P F
PF
D022082:Cellular Structures / Q000737:chemistry* / Q000502:physiology*; D015342:DNA Probes / Q000032:analysis / Q000502:physiology; D017781:Genes, Immediate-Early / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012313:RNA / Q000032:analysis / Q000502:physiology; D013997:Time Factors
D015342:DNA Probes; D012313:RNA