title: Effect of vitamin C supplementation on lipid peroxidation, muscle damage and inflammation after 30-min exercise at 75% VO2max.
abstract: OBJECTIVE
  Hypothetically, supplementation with the antioxidant vitamins C could alleviate exercise-induced lipid peroxidation. The purpose of this study was to evaluate the effect of vitamin C supplementation on exercise-induced lipid peroxidation, muscle damage and inflammation.
  
  
  METHODS
  Sixteen healthy untrained male volunteers participated in a 30-min exercise at 75% Vo2max. Subjects were randomly assigned to one of two groups: 1) placebo and 2) vitamin C (VC: 1 000 mg vitamin C). Blood samples were obtained prior to supplementation (baseline), 2 h after supplementation (immediately pre-exercise), post-exercise, 2 and 24 h after exercise. Plasma levels of VC, total antioxidant capacity (TAC), creatine kinase (CK), malondealdehyde (MDA), total leukocytes, neutrophils, lymphocytes, interleukin-6 (IL-6) and cortisol were measured.
  
  
  RESULTS
  Plasma vitamin C concentrations increased significantly in the VC in response to supplementation and exercise (P<0.05). TAC decreased significantly in Placebo group 24 h after exercise compared to pre-exercise (P<0.05). Although MDA levels were similar between groups at baseline, it increased significantly 2 h after exercise only in the Placebo group (P<0.05). CK increased immediately and 2 h after exercise in both groups and 24 h after exercise only in placebo group compared to pre-exercise (P<0.05). Markers of inflammation (total leukocyte counts, neutrophil counts and IL-6) were increased significantly in response to the exercise (P<0.05). In VC group, there was significant increase in lymphocyte counts immediately after exercise compared with pre-exercise (P<0.05). Serum cortisol concentrations significantly declined after supplementation compared with baseline (P<0.05) as well as declined 2 and 24 h after exercise compared with immediately after exercise in VC group (P<0.05).
  
  
  CONCLUSIONS
  VC supplementation prevented endurance exercise-induced lipid peroxidation and muscle damage but had no effect on inflammatory markers.
authors.affiliation: Department of Exercise Physiology, Guilan University, Rasht, Iran bnakhostin_aau@yahoo.com.
authors.forename: B
authors.initials: B
authors.lastname: Nakhostin-Roohi
authors.forename: P
authors.initials: P
authors.lastname: Babaei
authors.forename: F
authors.initials: F
authors.lastname: Rahmani-Nia
authors.forename: S
authors.initials: S
authors.lastname: Bohlooli
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000097:blood / Q000494:pharmacology*; D004311:Double-Blind Method; D005080:Exercise Test; D006801:Humans; D015850:Interleukin-6 / Q000097:blood; D007958:Leukocyte Count; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D018482:Muscle, Skeletal / Q000187:drug effects*; D009504:Neutrophils; D010101:Oxygen Consumption / Q000502:physiology
chemical_list: D000975:Antioxidants; D015850:Interleukin-6; D001205:Ascorbic Acid