title: Digestion of DNA with restriction endonucleases.
abstract: Restriction endonucleases recognize short DNA sequences and catalyze the cleavage of double-stranded DNA at specific sites within or adjacent to the recognition sequences. This unit describes how to cleave DNA and refers the investigator to frequently updated manuals by endonuclease suppliers for the precise optimized conditions. Alternate procedures are given for digesting a DNA sample with several different enzymes and digesting multiple DNA samples with the same endonuclease.
authors.affiliation: Massachusetts General Hospital, Boston, Massachusetts.
authors.forename: Kenneth D
authors.initials: KD
authors.lastname: Bloch
mesh_terms: D004247:DNA / Q000737:chemistry* / Q000235:genetics; D004262:DNA Restriction Enzymes / Q000737:chemistry*; D013379:Substrate Specificity
chemical_list: D004247:DNA; D004262:DNA Restriction Enzymes