Serum lipoprotein composition, lecithin cholesterol acyltransferase and tissue lipase activities in pregnant diabetic rats and their offspring receiving enriched n-3 PUFA diet.
The effects of dietary n-3 polyunsaturated fatty acids on lipoprotein concentrations and on lipoprotein lipase (LPL), hepatic triglyceride lipase (HTGL) and lecithin cholesterol acyltransferase (LCAT) activities were studied in streptozotocin-induced diabetic rats during pregnancy and in their macrosomic offspring from birth to adulthood. Pregnant diabetic and control rats were fed Isio-4 diet (vegetable oil) or EPAX diet (concentrated marine omega-3 EPA/DHA oil), the same diets were consumed by pups at weaning. Compared with control rats, diabetic rats showed, during pregnancy, a significant elevation in very low density lipoprotein (VLDL) and low and high density lipoprotein (LDL-HDL(1))-triglyceride, cholesterol and apoprotein B100 concentrations and a reduction in apoprotein A-I levels. HTGL activity was high while LPL and LCAT activities were low in these rats. The macrosomic pups of Isio-4-fed diabetic rats showed a significant enhancement in triglyceride and cholesterol levels at birth and during adulthood with a concomitant increase in lipase and LCAT activities. EPAX diet induces a significant diminution of VLDL and LDL-HDL(1) in mothers and in their macrosomic pups, accompanied by an increase in cholesterol and apoprotein A-I levels in HDL(2-3) fraction. It also restores LPL, HTGL and LCAT activities to normal range. EPAX diet ameliorates considerably lipoprotein disorders in diabetic mothers and in their macrosomic offspring.
Soulimane-Mokhtari
N A
NA
Laboratoire de Physiologie Animale et Biochimie, Département de Biologie, Faculté des Sciences, Université de Tlemcen, Algérie. nassima_amel@yahoo.fr
Guermouche
B
B
Saker
M
M
Merzouk
S
S
Merzouk
H
H
Hichami
A
A
Madani
S
S
Khan
N A
NA
Prost
J
J
D000273:Adipose Tissue / Q000201:enzymology; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000097:blood / Q000150:complications* / Q000178:diet therapy* / Q000378:metabolism; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage*; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D005260:Female; D005320:Fetal Macrosomia / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D008049:Lipase / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000378:metabolism*; D008074:Lipoproteins / Q000097:blood*; D008099:Liver / Q000201:enzymology; D008297:Male; D008431:Maternal-Fetal Exchange; D007862:Phosphatidylcholine-Sterol O-Acyltransferase / Q000097:blood*; D011247:Pregnancy; D011254:Pregnancy in Diabetics / Q000097:blood / Q000178:diet therapy* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D004042:Dietary Fats, Unsaturated; D015525:Fatty Acids, Omega-3; D008074:Lipoproteins; D007862:Phosphatidylcholine-Sterol O-Acyltransferase; D008049:Lipase; D008071:Lipoprotein Lipase