title: Metabolic labeling with amino acids.
abstract: Metabolic labeling techniques are used to study biosynthesis, processing, intracellular transport, secretion, degradation, and physical-chemical properties of proteins. This unit focuses on pulse-labeling and pulse-chase experiments done with [(35)S]-methionine, but gives directions for labeling with other radiolabeled amino acids, and also offers guidance for the safe use and handling of 35S-labeled compounds.
authors.affiliation: National Institute of Child Health and Human Development, NIH, Bethesda, Maryland, USA.
authors.forename: J S
authors.initials: JS
authors.lastname: Bonifacino
mesh_terms: D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism*; D007553:Isotope Labeling / Q000379:methods*; D008715:Methionine / Q000737:chemistry / Q000378:metabolism; D013462:Sulfur Radioisotopes / Q000737:chemistry
chemical_list: D000596:Amino Acids; D013462:Sulfur Radioisotopes; D008715:Methionine