Multiwalled carbon nanotube modified screen-printed electrodes for the detection of p-aminophenol: optimisation and application in alkaline phosphatase-based assays.
Carboxylated multiwalled carbon nanotubes (MWCNT-COOH) were used to modify the working electrode surface of different screen-printed electrodes. The effect of this modification on the electrodic characteristics (double layer capacitance, electroactive area and heterogeneous rate constants for the electron transfer) was evaluated and optimized for the cyclic voltammetric determination of p-aminophenol. The enzymatic hydrolysis of p-aminophenylphosphate was employed for the quantification of alkaline phosphatase, one of the most important label enzymes in immunoassays. Finally, ELISA assays were carried out to quantify pneumolysin using this enzymatic system. Results obtained indicated that low superficial densities of MWCNT-COOH (0.03-0.06 microg mm(-2)) yielded the same electrodic improvements but with better analytical properties.
Lamas-Ardisana
Pedro José
PJ
Departamento de Química Física y Analítica, Universidad de Oviedo, 33006 Oviedo, Asturias, Spain.
Queipo
Paula
P
Fanjul-Bolado
Pablo
P
Costa-García
Agustín
A
D000469:Alkaline Phosphatase / Q000032:analysis / Q000737:chemistry*; D000627:Aminophenols / Q000032:analysis*; D000814:Aniline Compounds / Q000737:chemistry; D001426:Bacterial Proteins / Q000032:analysis; D002138:Calibration; D004563:Electrochemistry; D004566:Electrodes; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006868:Hydrolysis; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013301:Streptolysins / Q000032:analysis*; D013499:Surface Properties
D000627:Aminophenols; D000814:Aniline Compounds; D001426:Bacterial Proteins; D037742:Nanotubes, Carbon; D009943:Organophosphorus Compounds; D013301:Streptolysins; C032290:plY protein, Streptococcus pneumoniae; C068798:4-aminophenylphosphate; D000469:Alkaline Phosphatase; C026729:4-aminophenol