title: Determination of antiovarian antibodies after laparoscopic ovarian electrocauterization in patients with polycystic ovary syndrome.
abstract: OBJECTIVE
  To determine whether ovarian damage consequent to laparoscopic ovarian electrocauterization may result in the development of humoral autoimmunity and production of antiovarian antibodies (AOA).
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Infertility and gynecologic endoscopy units of one of the medical university hospitals.
  
  
  METHODS
  Sixty-four reproductive-age infertile women (<or=35 y of age) with clomiphene citrate-resistant polycystic ovary syndrome (PCOS) and 50 fertile, healthy, reproductive-aged women (<or=35 y of age) as control group.
  
  
  METHODS
  Laparoscopic ovarian electrocauterization was performed under general anesthesia in clomiphene citrate-resistant PCOS patients for treatment of infertility.
  
  
  METHODS
  Serum samples from 52 patients (before and about 30-40 days after operation) and from 50 fertile healthy controls were assayed for determination of immunoglobulin class-specific AOA (IgM, IgG, and IgA) by using a commercially available ELISA kit that used human ovary as antigen.
  
  
  RESULTS
  A trend of rising in the level of AOA in all isotypes, IgM, IgG, and IgA, was found in sera after operation, compared with the case of sera obtained before operation. But when the ELISA value was calculated according to the formula recommended by the manufacturer, none of the patients were positive for AOA before the surgical intervention, and none of them developed positive AOA after operation. The overall ovulation rate was 81.3%, and the pregnancy rate was 67.2%.
  
  
  CONCLUSIONS
  The ovarian trauma consequent to laparoscopic ovarian electrocauterization in patients with resistant PCOS does not result in a significant production of AOA.
authors.affiliation: Department of Obstetrics and Gynecology, Division of Infertility and Gynecologic Endoscopy, Shiraz Institute of Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran. alborzis@sums.ac.ir
authors.forename: Saeed
authors.initials: S
authors.lastname: Alborzi
authors.forename: Fahimeh
authors.initials: F
authors.lastname: Tavazoo
authors.forename: Alamtaj Samsami
authors.initials: AS
authors.lastname: Dehaghani
authors.forename: Abbas
authors.initials: A
authors.lastname: Ghaderi
authors.forename: Soroosh
authors.initials: S
authors.lastname: Alborzi
authors.forename: Mehrnoosh
authors.initials: M
authors.lastname: Alborzi
mesh_terms: D000328:Adult; D001323:Autoantibodies / Q000032:analysis* / Q000097:blood; D016022:Case-Control Studies; D004564:Electrocoagulation / Q000009:adverse effects / Q000379:methods; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007247:Infertility, Female / Q000453:epidemiology / Q000209:etiology / Q000276:immunology; D010535:Laparoscopy / Q000009:adverse effects; D008599:Menstruation Disturbances / Q000453:epidemiology; D010053:Ovary / Q000276:immunology* / Q000601:surgery; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000276:immunology / Q000601:surgery*; D011183:Postoperative Complications / Q000453:epidemiology; D011247:Pregnancy; D016649:Primary Ovarian Insufficiency / Q000453:epidemiology / Q000209:etiology / Q000276:immunology; D055815:Young Adult
chemical_list: D001323:Autoantibodies