title: Genotoxic effects of white fluorescent light on human lymphocytes in vitro.
abstract: Sources of light beams such as white fluorescent light, are present in our daily life to meet the needs of life in the modern world. This study was conducted with the objective of determining the possible genotoxic, cytotoxic and aneugenic effects caused by this agent in different stages of the cell cycle (G0/early G1, S, and late G2), using different cytogenetic parameters (sister chromatid exchanges--SCE, chromosome aberrations--CA, and detection of aneugenic effects) in lymphocytes from temporary cultures of human peripheral blood. WFL showed a genotoxic effect in vitro, expressed by an increase in the frequency of SCE's, regardless of the cell cycle stage. However, no increase in the frequency of CAs was observed. In addition, disturbances in cell cycle kinetics and chromosomal segregation were also observed. Taken together, such data may contribute to a better understanding and a different management in the use of phototherapy for some pathological conditions.
authors.affiliation: Human Cytogenetics Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil.
authors.forename: Marúcia Irena Medeiros
authors.initials: MI
authors.lastname: Amorim
authors.forename: Iris
authors.initials: I
authors.lastname: Ferrari
authors.forename: Marcelo de Oliveira
authors.initials: Mde O
authors.lastname: Bahia
authors.forename: Patrícia Danielle Lima
authors.initials: PD
authors.lastname: Lima
authors.forename: Plínio Cerqueira Dos Santos
authors.initials: PC
authors.lastname: Cardoso
authors.forename: André Salim
authors.initials: AS
authors.lastname: Khayat
authors.forename: Isabel Rosa
authors.initials: IR
authors.lastname: Cabral
authors.forename: Rommel Rodríguez
authors.initials: RR
authors.lastname: Burbano
mesh_terms: D002453:Cell Cycle / Q000528:radiation effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D006801:Humans; D008027:Light / Q000009:adverse effects*; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008940:Mitotic Index; D011840:Radiation, Nonionizing / Q000009:adverse effects*; D012854:Sister Chromatid Exchange / Q000528:radiation effects*