title: STAT3 and STAT1 mediate IL-11-dependent and inflammation-associated gastric tumorigenesis in gp130 receptor mutant mice.
abstract: Deregulated activation of STAT3 is frequently associated with many human hematological and epithelial malignancies, including gastric cancer. While exaggerated STAT3 signaling facilitates an antiapoptotic, proangiogenic, and proproliferative environment for neoplastic cells, the molecular mechanisms leading to STAT3 hyperactivation remain poorly understood. Using the gp130(Y757F/Y757F) mouse model of gastric cancer, which carries a mutated gp130 cytokine receptor signaling subunit that cannot bind the negative regulator of cytokine signaling SOCS3 and is characterized by hyperactivation of the signaling molecules STAT1 and STAT3, we have provided genetic evidence that IL-11 promotes chronic gastric inflammation and associated tumorigenesis. Expression of IL-11 was increased in gastric tumors in gp130(Y757F/Y757F) mice, when compared with unaffected gastric tissue in wild-type mice, while gp130(Y757F/Y757F) mice lacking the IL-11 ligand-binding receptor subunit (IL-11Ralpha) showed normal gastric STAT3 activation and IL-11 expression and failed to develop gastric tumors. Furthermore, reducing STAT3 activity in gp130(Y757F/Y757F) mice, either genetically or by therapeutic administration of STAT3 antisense oligonucleotides, normalized gastric IL-11 expression and alleviated gastric tumor burden. Surprisingly, the genetic reduction of STAT1 expression also reduced gastric tumorigenesis in gp130(Y757F/Y757F) mice and coincided with reduced gastric inflammation and IL-11 expression. Collectively, our data have identified IL-11 as a crucial cytokine promoting chronic gastric inflammation and associated tumorigenesis mediated by excessive activation of STAT3 and STAT1.
authors.affiliation: Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Melbourne, Victoria, Australia. Matthias.Ernst@ludwig.edu.au
authors.forename: Matthias
authors.initials: M
authors.lastname: Ernst
authors.forename: Meri
authors.initials: M
authors.lastname: Najdovska
authors.forename: Dianne
authors.initials: D
authors.lastname: Grail
authors.forename: Therese
authors.initials: T
authors.lastname: Lundgren-May
authors.forename: Michael
authors.initials: M
authors.lastname: Buchert
authors.forename: Hazel
authors.initials: H
authors.lastname: Tye
authors.forename: Vance B
authors.initials: VB
authors.lastname: Matthews
authors.forename: Jane
authors.initials: J
authors.lastname: Armes
authors.forename: Prithi S
authors.initials: PS
authors.lastname: Bhathal
authors.forename: Norman R
authors.initials: NR
authors.lastname: Hughes
authors.forename: Eric G
authors.initials: EG
authors.lastname: Marcusson
authors.forename: James G
authors.initials: JG
authors.lastname: Karras
authors.forename: Songqing
authors.initials: S
authors.lastname: Na
authors.forename: Jonathon D
authors.initials: JD
authors.lastname: Sedgwick
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Hertzog
authors.forename: Brendan J
authors.initials: BJ
authors.lastname: Jenkins
mesh_terms: D000818:Animals; D050822:Cytokine Receptor gp130 / Q000235:genetics / Q000276:immunology*; D005753:Gastric Mucosa / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D017370:Interleukin-11 / Q000235:genetics / Q000276:immunology*; D015850:Interleukin-6 / Q000276:immunology; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D016376:Oligonucleotides, Antisense / Q000235:genetics / Q000378:metabolism; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D050794:STAT1 Transcription Factor / Q000235:genetics / Q000276:immunology*; D050796:STAT3 Transcription Factor / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction / Q000502:physiology; D013270:Stomach / Q000033:anatomy & histology / Q000473:pathology; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D017370:Interleukin-11; D015850:Interleukin-6; D016376:Oligonucleotides, Antisense; D021122:Protein Subunits; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; C494080:Stat1 protein, mouse; C494087:Stat3 protein, mouse; D050822:Cytokine Receptor gp130