title: Growth in liquid or solid media.
abstract: This appendix presents basic procedures for growing overnight (and larger) cultures, monitoring growth, and titering and isolating bacterial cultures by serial dilution. In addition, protocols are provided for isolating single colonies by streaking and spreading a plate. Replica plating and strain storage/retrieval is also detailed.
authors.affiliation: Clark and Elbing LLP, Boston, Massachusetts, USA.
authors.forename: K L
authors.initials: KL
authors.lastname: Elbing
authors.forename: R
authors.initials: R
authors.lastname: Brent
mesh_terms: D001419:Bacteria / Q000254:growth & development* / Q000302:isolation & purification; D001431:Bacteriological Techniques / Q000379:methods*; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*
chemical_list: D003470:Culture Media