title: Characterization of Rab27a and JFC1 as constituents of the secretory machinery of prostate-specific antigen in prostate carcinoma cells.
abstract: Prostate-specific antigen (PSA) and prostate-specific acid phosphatase (PSAP) are produced by prostate carcinoma cells. Their secretion has implications in both prostate cancer diagnosis and progression. The mechanisms involved in PSA and PSAP secretion in response to androgens have remained relatively unknown. The small GTPase Rab27a regulates exocytosis in several tissues. Here, we present methods for the characterization of Rab27a and its effector JFC1/Slp1 as key components of the secretory machinery that regulates exocytosis in prostate carcinoma cells.
authors.affiliation: Division of Biochemistry, Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Sergio D
authors.initials: SD
authors.lastname: Catz
mesh_terms: D000135:Acid Phosphatase; D045744:Cell Line, Tumor; D015536:Down-Regulation; D005089:Exocytosis; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D010539:Permeability; D017430:Prostate-Specific Antigen / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D025941:Protein Interaction Mapping / Q000379:methods*; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000502:physiology; D017027:Protein Tyrosine Phosphatases / Q000378:metabolism; D034741:RNA, Small Interfering / Q000494:pharmacology; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000075745:rab27 GTP-Binding Proteins
chemical_list: D008565:Membrane Proteins; D034741:RNA, Small Interfering; C431146:SYTL1 protein, human; C424133:Sytl1 protein, mouse; D033921:Vesicular Transport Proteins; D000075745:rab27 GTP-Binding Proteins; D000135:Acid Phosphatase; C428439:prostatic acid phosphatase; D017027:Protein Tyrosine Phosphatases; D017430:Prostate-Specific Antigen; C066694:RAB27A protein, human; D020691:rab GTP-Binding Proteins