Expression cloning of neural genes using Xenopus laevis oocytes.
Expression cloning requires a representative cDNA or genomic DNA library and a host organism in which the cloned genes can be transcribed and/or translated. It likewise requires a method to detect the expressed protein using, for example, the inherent biological activity of the gene or antibodies specific for the gene product. Most successful expression cloning strategies have employed cDNA libraries constructed in plasmid or bacteriophage lambda vectors and Xenopus oocytes or cultured mammalian cells as hosts. This unit presents several protocols designed for expression cloning paradigms that rely on electrophysiological recordings from Xenopus laevis oocytes.
Boulter
J
J
University of California at Los Angeles, Los Angeles, California, USA.
Boyer
C
C
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D009474:Neurons / Q000502:physiology*; D009865:Oocytes / Q000502:physiology*; D014982:Xenopus laevis