title: Honokiol, a natural therapeutic candidate, induces apoptosis and inhibits angiogenesis of ovarian tumor cells.
abstract: OBJECTIVE
  To observe the anti-tumor activities of honokiol on human ovarian tumor in vitro and in vivo.
  
  
  METHODS
  Cells were treated with honokiol, and the effects on proliferation and apoptosis were examined by MTT, DNA ladder, Hoechst staining, and flow cytometry assays. Expression of Bcl-2 members and caspase-3 were assessed. Measurements of tumor volume and microvessel densities (MVDs) were performed.
  
  
  RESULTS
  Honokiol significantly inhibited proliferation and induced apoptosis, with alteration of Bcl-2 members and caspase-3. Administration of honokiol to tumor-bearing animals decreased MVD and resulted in inhibition of tumor growth.
  
  
  CONCLUSIONS
  Honokiol could induce apoptosis and inhibit angiogenesis in vitro and in vivo, suggesting a novel and attractive therapeutic candidate for ovarian tumor treatment.
authors.affiliation: Department of Obstetrics and Gynecology, West China Second Hospital of Sichuan University, Renmin South Road, Chengdu 610041, People's Republic of China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Pan
authors.forename: Rutie
authors.initials: R
authors.lastname: Yin
authors.forename: Canhua
authors.initials: C
authors.lastname: Huang
authors.forename: Lijuan
authors.initials: L
authors.lastname: Chen
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D000498:Allyl Compounds; D010636:Phenols
chemical_list: D020533:Angiogenesis Inhibitors; D001713:Biphenyl Compounds; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols