Quantitative protein analysis using proteolytic [18O]water labeling.
This unit describes the procedure for proteolytic [(18)O]water labeling of peptides in order to quantitate relative protein levels for a comparative proteomic experiment.
Reynolds
Kristy J
KJ
University of Maryland, College Park, Maryland.
Fenselau
Catherine
C
University of Maryland, College Park, Maryland.
D000818:Animals; D006801:Humans; D007553:Isotope Labeling / Q000379:methods*; D015394:Molecular Structure; D010103:Oxygen Isotopes / Q000737:chemistry*; D010455:Peptides / Q000032:analysis / Q000235:genetics; D011506:Proteins / Q000032:analysis* / Q000235:genetics; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D014867:Water / Q000737:chemistry*
D010103:Oxygen Isotopes; D010455:Peptides; D011506:Proteins; D014867:Water