[The role of expired air moisture condensate in assessment of pulmonary inflammation in patients with chronic obstructive pulmonary disease].
OBJECTIVE
To study biochemical composition of expired air condensate (EAC) in patients with chronic obstructive pulmonary disease (COPD) in relation to a phase and severity of the disease and its treatment.


METHODS
EAC was investigated in 18 COPD patients and 9 healthy subjects. Basic broncholytic therapy with ipratropium bromide was combined with beclomethasone and fenspiride in 11 and 7 patients, respectively. The condensate was lyophilised, the residue was solved and analysed on the biochemical analyzer Casis (Beringer Manheim, Rosch). EAC was examined for albumin, C-reactive protein, glucose, cholesterol, triglycerides, urea, uric acid, alkaline phosphatase (AP), lactate dehydrogenase, gamma-glutamyl transferase, total calcium, magnesium.


RESULTS
Compared to healthy subjects, COPD patients' EAC contains significantly higher levels of albumin, C-reactive protein, calcium, bilirubin and more active AP. Quantitative composition of EAC depends on COPD phase and severity. A negative correlation exists between FEV+AEA-1 and albumin concentration, FEV+AEA-1 and CRP concentration. The anti-inflammatory therapy decreases EAC content of both protein and lipid metabolism products, enzyme activity reflecting attenuation of oxidant and inflammatory processes, stabilization of cell membranes in the respiratory zone.


CONCLUSIONS
EAC composition reflects metabolic processes in the lungs and can be used for assessment of airway affection, activity of the inflammatory process and COPD treatment efficacy.
Dotsenko
E K
EK
Goncharova
V A
VA
Kuzubova
N A
NA
Kamenova
M Iu
MIu
Egorova
N V
NV
D000388:Air / Q000032:analysis*; D000418:Albumins / Q000032:analysis; D000469:Alkaline Phosphatase / Q000032:analysis; D001663:Bilirubin / Q000032:analysis; D015415:Biomarkers / Q000032:analysis*; D001944:Breath Tests / Q000379:methods; D002097:C-Reactive Protein / Q000032:analysis; D002118:Calcium / Q000032:analysis; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D029424:Pulmonary Disease, Chronic Obstructive / Q000175:diagnosis / Q000378:metabolism*; D012720:Severity of Illness Index
D000418:Albumins; D015415:Biomarkers; D002097:C-Reactive Protein; D000469:Alkaline Phosphatase; D001663:Bilirubin; D002118:Calcium