ACL-I, a lectin from the marine sponge Axinella corrugata: isolation, characterization and chemotactic activity.
The lectin from the marine sponge Axinella corrugata (ACL-I) was purified by affinity chromatography on rabbit erythrocytic stroma incorporated into a polyacrylamide gel followed by gel filtration on Ultrogel AcA 44 column. Purified ACL-I is a hexameric glycoprotein with a Mr of 82.3 kDa estimated by SDS-PAGE and 78.5 kDa by FPLC on Superose 12 HR column. The pI of lectin is 6.3 and ACL-I is constituted of 13.9 kDa similar subunits some of them linked by disulphide bridges. This lectin agglutinates native rabbit, goat and dog erythrocytes and in less extent human erythrocytes. The hemagglutinating activity is independent of Ca(2+), Mg(2+) and Mn(2+), but it is strongly inhibited by carbohydrates containing N-acetyl groups. ACL-I is stable up to 70 degrees C for 30 min, with optimum pH between 7 and 8, and it is also resistant to enzymatic proteolysis in vitro. In the presence of reducing or denaturant agents, the lectin activity decreases. ACL-I displays chemotactic effect on rat neutrophil in vitro which is inhibited by N-acetyl-d-glucosamine.
Dresch
Roger R
RR
Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Zanetti
Gilberto D
GD
Lerner
Cléa B
CB
Mothes
Beatriz
B
Trindade
Vera M T
VM
Henriques
Amélia T
AT
Vozári-Hampe
Magdolna M
MM
D000818:Animals; D047089:Axinella / Q000737:chemistry*; D002630:Chemotactic Factors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002634:Chemotaxis, Leukocyte; D002846:Chromatography, Affinity; D004220:Disulfides / Q000302:isolation & purification; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004912:Erythrocytes / Q000187:drug effects; D006041:Goats; D006384:Hemagglutination; D006388:Hemagglutinins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006358:Hot Temperature; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D008970:Molecular Weight; D009504:Neutrophils / Q000187:drug effects; D011489:Protein Denaturation; D021122:Protein Subunits; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar
D002630:Chemotactic Factors; D004220:Disulfides; D006388:Hemagglutinins; D037102:Lectins; D021122:Protein Subunits