title: Nasal chondrocytes and fibrin sealant for cartilage tissue engineering.
abstract: Hybrid constructs associating a biodegradable matrix and autologous chondrocytes hold promise for the treatment of articular cartilage defects. In this context, our objective was to investigate the potential use of nasal chondrocytes associated with a fibrin sealant for the treatment of articular cartilage defects. The phenotype of primary nasal chondrocytes (NC) from human (HNC) and rabbit (RNC) origin were characterized by RT-PCR. The ability of constructs associating fibrin sealant and NC to form a cartilaginous tissue in vivo was investigated, firstly in a subcutaneous site in nude mice and secondly in an articular cartilage defect in rabbit. HNC express type II collagen and aggrecan, the two major hallmarks of a chondrocytic phenotype. Furthermore, when injected subcutaneously into nude mice within a fibrin sealant, these chondrocytes were able to form a cartilage-like tissue. Our data indicate that RNC also express type II collagen and aggrecan and maintained their phenotype in three-dimensional culture within a fibrin sealant. Moreover, treatment of rabbit articular cartilage defects with autologous RNC embedded in a fibrin sealant led to the formation of a hyalin-like repair tissue. The use of fibrin sealant containing hybrid autologous NC therefore appears as a promising approach for cell-based therapy of articular cartilage.
authors.affiliation: INSERM, U 791, Laboratory for Osteo-articular and Dental Tissue Engineering, University of Nantes, 1 place Alexis Ricordeau, Nantes, 44042, France.
authors.forename: C
authors.initials: C
authors.lastname: Vinatier
authors.forename: O
authors.initials: O
authors.lastname: Gauthier
authors.forename: M
authors.initials: M
authors.lastname: Masson
authors.forename: O
authors.initials: O
authors.lastname: Malard
authors.forename: A
authors.initials: A
authors.lastname: Moreau
authors.forename: B H
authors.initials: BH
authors.lastname: Fellah
authors.forename: M
authors.initials: M
authors.lastname: Bilban
authors.forename: R
authors.initials: R
authors.lastname: Spaethe
authors.forename: G
authors.initials: G
authors.lastname: Daculsi
authors.forename: J
authors.initials: J
authors.lastname: Guicheux
mesh_terms: D053674:Aggrecans / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism; D002358:Cartilage, Articular / Q000166:cytology / Q000473:pathology; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology* / Q000637:transplantation; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D015718:Fibrin Tissue Adhesive / Q000378:metabolism*; D048091:Guided Tissue Regeneration / Q000379:methods; D006801:Humans; D020007:Implants, Experimental; D051379:Mice; D008819:Mice, Nude; D009300:Nasal Septum / Q000166:cytology*; D010641:Phenotype; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D014182:Transplantation, Autologous
chemical_list: D053674:Aggrecans; D001672:Biocompatible Materials; D024043:Collagen Type II; D015718:Fibrin Tissue Adhesive