title: Purification of human IgA.
abstract: This unit describes the purification of human immunoglobulin A (IgA).The main method utilizes an IgA-binding protein (IgA-bp) as an affinity reagent, similar to the IgG-binding proteins, protein A and protein G. An alternate protocol describes a method for isolating IgA1 using affinity column chromatography with Jacalin, an IgA1-specific lectin. Another alternate protocol describes IgA purification by conventional fractionation using size-exclusion chromatography.
authors.affiliation: Applied Biotech, San Diego, California.
authors.forename: Todd D
authors.initials: TD
authors.lastname: Pack
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D006801:Humans; D007070:Immunoglobulin A / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000276:immunology; D037121:Plant Lectins / Q000737:chemistry* / Q000276:immunology; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000276:immunology
chemical_list: C029152:G-substrate; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D037121:Plant Lectins; D013205:Staphylococcal Protein A; C043696:jacalin