title: New biological indicators to evaluate and monitor radiation-induced damage: an accident case report.
abstract: The aim of this work was to use several new biological indicators to evaluate damage to the main physiological systems in a victim exposed accidentally to ionizing radiation. Blood samples were used for biological dosimetry and for measurement of the plasma concentrations of several molecules: Flt3 ligand to assess the hematopoietic system, citrulline as an indicator of the digestive tract, and several oxysterols as lipid metabolism and vascular markers. The cytogenetic evaluation estimated the dose to the victim to be between 4.2 and 4.8 Gy, depending on the methodology used. Monitoring the Flt3 ligand demonstrated the severity of bone marrow aplasia. In contrast, the citrulline concentration showed the absence of gastrointestinal damage. Variations in oxysterol concentrations suggested radiation-induced damage to the liver and the cardiovascular system. These results were correlated with those from classic biochemical markers, which demonstrated severe damage to the hematopoietic system and suggested the appearance of subclinical damage to the liver and cardiovascular system. These results demonstrate for the first time the importance of a multiparameter biological approach in the evaluation of radiation damage after accidental irradiation.
authors.affiliation: Institut de radioprotection et de surete nuclea, Fontenay aux roses, France. jean-marc.berto@irsn.fr
authors.forename: J M
authors.initials: JM
authors.lastname: Bertho
authors.forename: L
authors.initials: L
authors.lastname: Roy
authors.forename: M
authors.initials: M
authors.lastname: Souidi
authors.forename: M
authors.initials: M
authors.lastname: Benderitter
authors.forename: Y
authors.initials: Y
authors.lastname: Gueguen
authors.forename: J J
authors.initials: JJ
authors.lastname: Lataillade
authors.forename: M
authors.initials: M
authors.lastname: Prat
authors.forename: T
authors.initials: T
authors.lastname: Fagot
authors.forename: T
authors.initials: T
authors.lastname: De Revel
authors.forename: P
authors.initials: P
authors.lastname: Gourmelon
mesh_terms: D015415:Biomarkers / Q000097:blood*; D001772:Blood Cell Count; D002319:Cardiovascular System / Q000528:radiation effects; D002465:Cell Movement / Q000528:radiation effects; D002956:Citrulline / Q000097:blood; D003933:Diagnosis; D005500:Follow-Up Studies; D041981:Gastrointestinal Tract / Q000528:radiation effects; D006410:Hematopoiesis / Q000528:radiation effects*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000528:radiation effects; D018788:Radioactive Hazard Release; D011874:Radiometry
chemical_list: D015415:Biomarkers; D002956:Citrulline