title: A genome-wide functional investigation into the roles of receptor-like proteins in Arabidopsis.
abstract: Receptor-like proteins (RLPs) are cell surface receptors that typically consist of an extracellular leucine-rich repeat domain, a transmembrane domain, and a short cytoplasmatic tail. In several plant species, RLPs have been found to play a role in disease resistance, such as the tomato (Solanum lycopersicum) Cf and Ve proteins and the apple (Malus domestica) HcrVf2 protein that mediate resistance against the fungal pathogens Cladosporium fulvum, Verticillium spp., and Venturia inaequalis, respectively. In addition, RLPs play a role in plant development; Arabidopsis (Arabidopsis thaliana) TOO MANY MOUTHS (TMM) regulates stomatal distribution, while Arabidopsis CLAVATA2 (CLV2) and its functional maize (Zea mays) ortholog FASCINATED EAR2 regulate meristem maintenance. In total, 57 RLP genes have been identified in the Arabidopsis genome and a genome-wide collection of T-DNA insertion lines was assembled. This collection was functionally analyzed with respect to plant growth and development and sensitivity to various stress responses, including susceptibility toward pathogens. A number of novel developmental phenotypes were revealed for our CLV2 and TMM insertion mutants. In addition, one AtRLP gene was found to mediate abscisic acid sensitivity and another AtRLP gene was found to influence nonhost resistance toward Pseudomonas syringae pv phaseolicola. This genome-wide collection of Arabidopsis RLP gene T-DNA insertion mutants provides a tool for future investigations into the biological roles of RLPs.
authors.affiliation: Plant Research International, B.V., Business Unit of Bioscience, 6700 AA Wageningen, The Netherlands.
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.forename: Ursula
authors.initials: U
authors.lastname: Ellendorff
authors.forename: Ben
authors.initials: B
authors.lastname: Kemp
authors.forename: John W
authors.initials: JW
authors.lastname: Mansfield
authors.forename: Alec
authors.initials: A
authors.lastname: Forsyth
authors.forename: Kathy
authors.initials: K
authors.lastname: Mitchell
authors.forename: Kubilay
authors.initials: K
authors.lastname: Bastas
authors.forename: Chun-Ming
authors.initials: CM
authors.lastname: Liu
authors.forename: Alison
authors.initials: A
authors.lastname: Woods-Tör
authors.forename: Cyril
authors.initials: C
authors.lastname: Zipfel
authors.forename: Pierre J G M
authors.initials: PJ
authors.lastname: de Wit
authors.forename: Jonathan D G
authors.initials: JD
authors.lastname: Jones
authors.forename: Mahmut
authors.initials: M
authors.lastname: Tör
authors.forename: Bart P H J
authors.initials: BP
authors.lastname: Thomma
mesh_terms: D017360:Arabidopsis / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology*; D018745:Genome, Plant; D016254:Mutagenesis, Insertional
chemical_list: D029681:Arabidopsis Proteins