title: Preparation of 2'-deoxy-2'-methylseleno-modified phosphoramidites and RNA.
abstract: The derivatization of nucleic acids with selenium is a useful approach to facilitate phase determination during three-dimensional structural analysis by X-ray crystallography. This unit describes (1) the synthesis and characterization of 2'-deoxy-2'-methylseleno (2'-Se-methyl) nucleosides and their corresponding 3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite derivatives, (2) the site-specific incorporation of 2'-Se-methyl ribonucleosides into oligoribonucleotides by chemical RNA solid-phase synthesis, and (3) the enzymatic ligation of Se-containing RNA oligonucleotides to produce a biologically relevant RNA sequence.
authors.affiliation: Leopold-Franzens University, Innsbruck, Austria.
authors.forename: Ronald
authors.initials: R
authors.lastname: Micura
authors.forename: Claudia
authors.initials: C
authors.lastname: Höbartner
authors.forename: Renate
authors.initials: R
authors.lastname: Rieder
authors.forename: Christoph
authors.initials: C
authors.lastname: Kreutz
authors.forename: Barbara
authors.initials: B
authors.lastname: Puffer
authors.forename: Kathrin
authors.initials: K
authors.lastname: Lang
authors.forename: Holger
authors.initials: H
authors.lastname: Moroder
mesh_terms: D000577:Amides / Q000138:chemical synthesis* / Q000737:chemistry; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D016566:Organoselenium Compounds / Q000737:chemistry*; D010756:Phosphoric Acids / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D000577:Amides; D016566:Organoselenium Compounds; D010756:Phosphoric Acids; D012313:RNA; C008493:methylselenic acid; C011067:phosphoramidic acid