Photoinduced in situ formation of various F-actin assemblies with a photoresponsive polycation.
We developed a novel in situ method for the control of F-actin assembly by using a synthetic photoresponsive polycation. The photoresponsive polycation mainly comprises a water-soluble cationic monomer and also contains a small amount of the monomer of a triphenylmethane leucohydroxide derivative (20 mol %), which is a well-known photochromic molecule that can be cationized in aqueous solution by ultra violet (UV) irradiation, thereby causing an increase in the total charge on the photoresponsive polycation. Thus, by exposure to UV radiation in aqueous solution, F-actin and the photoresponsive polycation start assembling into F-actin/photoresponsive polycation complexes of various morphologies such as bundles, coils, and networks, depending upon the concentrations of both the F-actin and salt. Further, localized UV irradiation can be applied in order to control the local formation of F-actin/photoresponsive polycation complexes. Thus, this technique provides a novel method for the spatiotemporal control of F-actin assembly and can be applied to investigate the unknown characteristics of F-actin.
Misu
Manami
M
Department of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-8628, Japan.
Furukawa
Hidemitsu
H
Kwon
Hyuck-Joon
HJ
Shikinaka
Kazuhiro
K
Kakugo
Akira
A
Satoh
Toshifumi
T
Osada
Yoshihito
Y
Gong
Jian Ping
JP
D000199:Actins / Q000378:metabolism* / Q000528:radiation effects* / Q000648:ultrastructure; D000818:Animals; D008856:Microscopy, Fluorescence; D011073:Polyamines / Q000494:pharmacology*; D000071228:Polyelectrolytes; D014466:Ultraviolet Rays
D000199:Actins; D011073:Polyamines; D000071228:Polyelectrolytes