title: [Molecular characterization of hantavirus Zhejiang isolate ZT10 strain from M. fartis].
abstract: OBJECTIVE
  To learn about the complete genomic sequence of the Seoul virus strain ZT10 isolated from M. fartis.
  
  
  METHODS
  The total RNA was extracted from the infected Vero E6 cells and amplified by RT-PCR. The purified PCR products were cloned into T-vector and sequenced.
  
  
  RESULTS
  The results demonstrated that the complete genome of ZT10 was comprised of L(6530), M(3651) and S(1753) segments which encoded 2151-1133 and 429 amino acids respectively.
  
  
  CONCLUSIONS
  Analysis of sequence revealed that the ZT10 belonged to Seoul virus. The nucleotide sequence identity of the M gene with Seoul virus was 84.0%-96.3%. The identity with Hantan vrisu (Prospect Hill virus, Tula virus) isolated from M. fartis was 57.5%-60.9%. The sequence identity of the S gene with Seoul virus was 87.9%-96.0% at nucleotide level and 96.9%-97.9% at amino acid level.
authors.affiliation: Zhejiang Provincial Center for Disease Prevention and Control, Hangzhou 310009, China. Jingqingw@yahoo.com.cn
authors.forename: Jing-qing
authors.initials: JQ
authors.lastname: Weng
authors.forename: Rong-hui
authors.initials: RH
authors.lastname: Xie
authors.forename: Ping-ping
authors.initials: PP
authors.lastname: Yao
authors.forename: Ming-hong
authors.initials: MH
authors.lastname: Li
authors.forename: Fang
authors.initials: F
authors.lastname: Xu
authors.forename: Yi-yu
authors.initials: YY
authors.lastname: Lu
authors.forename: Han-ping
authors.initials: HP
authors.lastname: Zhu
authors.forename: Qing-rong
authors.initials: QR
authors.lastname: Cui
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D019085:Fluorescent Antibody Technique, Direct; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D000956:Antigens, Viral; D018076:DNA, Complementary; D004279:DNA, Viral