[Expression of telomere binding factor 2 (TRF2) on leukemia cell lines and primary leukemia cells].
To detect the expression levels of telomere binding factor 2 (TRF2) on leukemia cell lines and primary leukemia cells.



The expression of TRF2 mRNA was detected with quantitative real-time RT-PCR in leukemia cell lines and primary leukemia cells. The Western blot analysis was used for the detection of TRF2 protein expression.



TRF2 was overexpressed in T-cell leukemia cell lines but not in myelogenous leukemia cell lines. Significant higher expression levels of TRF2 were observed in primary leukemia cells from patients with M0 and M1 subtypes of acute myelogenous leukemia (AML) compared with normal control and other subtypes of AML.



Increased TRF2 expression levels are found in T-cell leukemia cell lines and AML patients with poor prognosis, which suggests that TRF2 expression might be related to the prognosis of leukemia.
Chen
Xiao-hui
XH
The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.
Tong
Yin
Y
Xu
Wei-lai
WL
Jin
Jie
J
Qian
Wen-bin
WB
D000293:Adolescent; D000328:Adult; D005260:Female; D018922:HL-60 Cells; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D015470:Leukemia, Myeloid, Acute / Q000378:metabolism; D015458:Leukemia, T-Cell / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D035341:Telomeric Repeat Binding Protein 2 / Q000378:metabolism*; D055815:Young Adult
D012333:RNA, Messenger; C504753:TERF2 protein, human; D035341:Telomeric Repeat Binding Protein 2