Development of a new bioprocess for production of 1,3-propanediol I.: Modeling of glycerol bioconversion to 1,3-propanediol with Klebsiella pneumoniae enzymes.
Glycerol is a renewable resource for it is formed as a byproduct during biodiesel production. Because of its large volume production, it seems to be a good idea to develop a technology that converts this waste into products of high value, for example, to 1,3-propanediol (1,3-PD). We suggested an enzymatic bioconversion in a membrane reactor in which the NAD coenzyme can be regenerated, and three key enzymes are retained by a 10-kDa ultrafilter membrane. Unfortunately, some byproducts also formed during successful glycerol to 1,3-PD bioconversion runs, as we used crude enzyme solution of Klebsiella pneumoniae. To study the possibilities to avoid this byproduct formation, we built a mathematical description of this system. The model was also used for simulation bioconversions of high glycerol concentration with and without elimination of byproduct formation and of continuous operation.
Németh
Aron
A
Department of Agricultural Chemical Technology, Budapest University of Technology and Economics, Budapest, Hungary.
Sevella
Béla
B
D019149:Bioreactors; D001709:Biotechnology; D001711:Biotransformation; D005990:Glycerol / Q000378:metabolism*; D007711:Klebsiella pneumoniae / Q000201:enzymology*; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D011409:Propylene Glycols / Q000378:metabolism*
D011409:Propylene Glycols; D009243:NAD; C041787:1,3-propanediol; D005990:Glycerol