The polymerase chain reaction.
This appendix describes the methodology behind PCR and gives a method for amplifying DNA enzymatically by the PCR and for optimizing this reaction for the sequence and primer set of interest.
Kramer
Martha F
MF
Harvard Medical School, Boston, Massachusetts.
Coen
Donald M
DM
Harvard Medical School, Boston, Massachusetts.
D000818:Animals; D004247:DNA / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D019914:Taq Polymerase / Q000378:metabolism
D004247:DNA; D019914:Taq Polymerase