Oxidative injury and neuropathy in diabetes and impaired glucose tolerance.
Clinical studies suggest that impaired glucose tolerance (IGT) is associated with the development of neuropathy. The aim of the current study was to determine if neuropathy developed in the female Zucker Diabetic Fatty (ZDF) rat, an animal model of IGT and type 2 diabetes. The ZDF rat develops impaired glucose tolerance (IGT) when fed a control diet, and frank diabetes when fed a high fat diet. Following 10 weeks of hyperglycemia, sensory nerve action potentials (SNAP) and compound motor action potentials (CMAP) were reduced and sensory conduction velocities were slowed (distal>proximal) in the tail and hind limb in ZDF animals with IGT and frank diabetes (p<0.01). Neuropathy was coupled with evidence of increased reactive oxygen species (ROS) and cellular injury in dorsal root ganglion (DRG) neurons from IGT animals. Our study supports the hypothesis that neuropathy develops in an animal model of IGT and is associated with evidence of oxidative injury in DRG and peripheral nerves.
Russell
James W
JW
Department of Neurology, University of Maryland, 22 South Greene Street, Baltimore, MD 21201, USA; Department of Anatomy and Neurobiology, University of Maryland, 22 South Greene Street, Baltimore, MD 21201, USA; Veterans Affairs Medical Center, Baltimore, MD, USA. Electronic address: JRussell@som.umaryland.edu.
Berent-Spillson
Alison
A
Molecular and Behavioral Neuroscience Institute, University of Michigan, USA.
Vincent
Andrea M
AM
Department of Neurology, University of Michigan, USA.
Freimann
Catherine L
CL
Novartis Pharmaceuticals, East Hanover, New Jersey, USA.
Sullivan
Kelli A
KA
Department of Neurology, University of Michigan, USA.
Feldman
Eva L
EL
Department of Neurology, University of Michigan, USA.
D000818:Animals; D001786:Blood Glucose / Q000235:genetics / Q000378:metabolism; D003920:Diabetes Mellitus / Q000097:blood* / Q000235:genetics / Q000473:pathology; D003929:Diabetic Neuropathies / Q000097:blood* / Q000235:genetics / Q000473:pathology; D005260:Female; D005727:Ganglia, Spinal / Q000378:metabolism / Q000473:pathology; D018149:Glucose Intolerance / Q000097:blood* / Q000235:genetics / Q000473:pathology; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D011924:Rats, Zucker
D001786:Blood Glucose