Novel automated extraction method for quantitative analysis of urinary 11-nor-delta(9)-tetrahydrocannabinol-9-carboxylic acid (THC-COOH).
An automated extraction method for extracting the major urinary metabolite of cannabis, 11-nor-Delta(9)-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) was developed on the four-probe Gilson ASPEC XL4trade mark solid-phase extraction (SPE) system. The method works on liquid-liquid extraction principles but does not require the use of SPE cartridges. The limits of detection and quantitation and the upper limit of linearity (ULOL) of the developed method were found to be 1, 2, and 1,500 ng/mL, respectively. There was no detectable carry over after 10,000 ng/mL analyte. For a batch of 76 samples, the process uses less than 100 mL methanol, 450 mL extracting solvent hexane/ethyl acetate (5:1, v/v) and 1 L rinsing solvent, 30% methanol in water. The automated extraction process takes 5 h to complete. Precision and accuracy of the method are comparable to both manual liquid-liquid extraction and automated SPE methods. The method has proven to be a simple, speedy, and economical alternative to the currently popular automated SPE method for the quantitative analysis of urinary THC-COOH.
Fu
Shanlin
S
Toxicology Unit, Pacific Laboratory Medicine Services, Northern Sydney Central Coast Area Health Services, New South Wales, Australia 2113. shanlin.fu@uts.edu.au
Lewis
John
J
D001331:Automation; D013759:Dronabinol / Q000031:analogs & derivatives* / Q000652:urine; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D006868:Hydrolysis; D002189:Marijuana Abuse
D013759:Dronabinol; C025351:delta(9)-tetrahydrocannabinolic acid