title: Characterization of tertiary folding of RNA by circular dichroism and urea.
abstract: CD spectroscopy can be used to monitor RNA tertiary folding transitions that may not be observable by absorbance spectroscopy. With the use of computer-controlled titrators, data can be acquired rapidly, and accurate thermodynamic properties can be obtained over a wide variety of conditions. Thus, CD spectroscopy provides a useful complement to site-resolved or chemical modification methods.
authors.affiliation: University of Chicago, Chicago, Illinois, USA.
authors.forename: T R
authors.initials: TR
authors.lastname: Sosnick
mesh_terms: D002942:Circular Dichroism / Q000379:methods*; D008274:Magnesium / Q000494:pharmacology; D009690:Nucleic Acid Conformation / Q000187:drug effects; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D013816:Thermodynamics; D017186:Titrimetry; D014508:Urea / Q000737:chemistry*
chemical_list: D012313:RNA; D014508:Urea; D008274:Magnesium