Culture of HIV in monocytes and macrophages.
Monocytes and macrophages constitute important cellular targets for macrophage-tropic (M-tropic) strains of HIV-1 and are believed to play a major role in the pathogenesis of AIDS. This updated unit provides procedures for culturing HIV in these target cells under suspension and adherent cell culture conditions. The unit also provides methods for expanding M-tropic primary isolates of HIV and determining the 50% tissue culture infectious dose (TCID50) of HIV stocks.
Crowe
Suzanne
S
Macfarlane Burnet Institute for Medical Research and Monash University, Melbourne, Australia.
Maslin
Clare
C
Macfarlane Burnet Institute for Medical Research and Monash University, Melbourne, Australia.
Ellery
Philip
P
Macfarlane Burnet Institute for Medical Research and Monash University, Melbourne, Australia.
Kedzierska
Katherine
K
University of Melbourne, Melbourne, Australia.
D018929:Cell Culture Techniques; D002478:Cells, Cultured; D006678:HIV / Q000254:growth & development*; D006801:Humans; D008264:Macrophages / Q000821:virology*; D009000:Monocytes / Q000821:virology*; D014773:Virology / Q000379:methods*