Infectious diseases testing.
Molecular methods have been applied widely for the diagnosis of infectious diseases. Beginning with solution hybridization in the early 1990s, multiple methods for nucleic acid hybridization and amplification have been introduced into the laboratory for the identification and characterization of microbial pathogens. This unit contains examples of several basic approaches for microbial detection or characterization in the laboratory. Methods in this chapter include automated nucleic acid extraction, direct detection of microbial pathogens, and characterization of pathogens by DNA sequencing or typing. Any of these methods could be customized for the characterization of bacteria, fungi, parasites, or viruses.
Blakey
Gregory L
GL
Baylor College of Medicine, Houston, Texas, USA.
Luna
Ruth Ann
RA
Versalovic
James
J
D003141:Communicable Diseases / Q000175:diagnosis* / Q000235:genetics; D006801:Humans; D009693:Nucleic Acid Hybridization; D020133:Reverse Transcriptase Polymerase Chain Reaction