Molecular characterization of surfactant-driven microbial community changes in anaerobic phenanthrene-degrading cultures under methanogenic conditions.
SDS and Triton X-100 added at their critical micelle concentrations (CMCs), increased phenanthrene solubility in the presence of sediments and inhibited phenanthrene biodegradation. Triton X-100 caused more inhibition than SDS. 16S rDNA analyses revealed that both surfactants changed the microbial communities of phenanthrene-degrading cultures. Further, after the surfactant additions, parts of the microbial populations were not detected and methane production decreased. Surfactant applications, necessary to achieve actual CMCs, alter microbial community structure and diminish methanogenic activity under anaerobic conditions. We propose that this change may be related to the inhibitory effects of SDS and Triton X-100 on phenanthrene biodegradation under methanogenic conditions.
Chang
Matthew Wook
MW
School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637459 Singapore. matthewchang@ntu.edu.sg
Holoman
Tracey Pulliam
TP
Yi
Hyunmin
H
D000693:Anaerobiosis / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental / Q000187:drug effects; D004275:DNA, Ribosomal / Q000235:genetics; D008697:Methane / Q000378:metabolism*; D008823:Micelles; D017830:Octoxynol / Q000494:pharmacology; D010616:Phenanthrenes / Q000378:metabolism*; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology*
D004275:DNA, Ribosomal; D008823:Micelles; D010616:Phenanthrenes; D013501:Surface-Active Agents; D012967:Sodium Dodecyl Sulfate; C031181:phenanthrene; D017830:Octoxynol; D008697:Methane