Expression of neutrophil gelatinase-associated lipocalin in calcium-induced keratinocyte differentiation.
In a previous search for the differentially expressed genes in keratinocyte differentiation, we identified neutrophil gelatinase-associated lipocalin (NGAL) as a calcium-induced gene. In this study, we further verified the expression of NGAL in cultured keratinocytes as well as in several skin diseases. Reverse transcription-polymerase chain reaction (RT-PCR), Western blot, and ELISA clearly showed that NGAL expression was markedly increased in calcium-induced keratinocyte differentiation in vitro. However, in our previous report, NGAL expression was not detected in normal skin tissue except for hair follicle by in situ hybridization and immunohistochemistry, indicating the difference of cell status between in vitro and in vitro conditions. Interestingly, NGAL expression was highly increased in psoriasis-like inflammatory disorders (lichen planus and pityriasis rubura pilaris) and skin cancers (keratoacanthoma and squamous cell carcinoma), implying that NGAL may be related with the epidermal hyperplasia. Collectively, these results reveal the potential importance of NGAL in the maintenance of skin homeostasis.
Lee
Jeung-Hoon
JH
Department of Dermatology, School of Medicine, Chungnam National University, 640 Daesa-dong, Jung-gu, Daejeon, Korea.
Kye
Kyung-Chae
KC
Seo
Eun-Young
EY
Lee
Kyungmoon
K
Lee
Sang-Keun
SK
Lim
Jong-Soon
JS
Seo
Young-Joon
YJ
Kim
Chang Deok
CD
Park
Jang-Kyu
JK
D000209:Acute-Phase Proteins / Q000096:biosynthesis*; D002118:Calcium / Q000378:metabolism*; D002454:Cell Differentiation; D003470:Culture Media; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D005786:Gene Expression Regulation; D006706:Homeostasis; D006801:Humans; D015603:Keratinocytes / Q000201:enzymology; D000071068:Lipocalin-2; D054834:Lipocalins / Q000096:biosynthesis*; D008954:Models, Biological; D011518:Proto-Oncogene Proteins / Q000096:biosynthesis*; D011565:Psoriasis / Q000201:enzymology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism*; D012878:Skin Neoplasms / Q000201:enzymology
D000209:Acute-Phase Proteins; D003470:Culture Media; D017077:Culture Media, Conditioned; C489506:LCN2 protein, human; D000071068:Lipocalin-2; D054834:Lipocalins; D011518:Proto-Oncogene Proteins; D002118:Calcium