title: A high-resolution large area serotonin map of a live rat brain section.
abstract: We employ three-photon microscopy to produce a high-resolution map of serotonin autofluorescence in a rat midbrain section (covering more than half of the brain), to quantitatively characterize serotonin distribution and release in different areas of a live brain slice. The map consists of a tiling of approximately 160 contiguous optical images (covering an area of approximately 27 mm with sub-mum resolution in 20 min), and is recorded before and after inducing depolarization. We observe that the total serotonin exocytosed from the somata in the raphe is quantitatively comparable with regions containing a high density of serotonergic processes. Our results demonstrate that high-resolution, wide-area, dynamic neurotransmitter mapping is now possible.
authors.affiliation: Department of Chemical Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai, India.
authors.forename: Sanjeev K
authors.initials: SK
authors.lastname: Kaushalya
authors.forename: Suman
authors.initials: S
authors.lastname: Nag
authors.forename: Himanish
authors.initials: H
authors.lastname: Ghosh
authors.forename: Senthil
authors.initials: S
authors.lastname: Arumugam
authors.forename: Sudipta
authors.initials: S
authors.lastname: Maiti
mesh_terms: D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D001931:Brain Mapping / Q000379:methods*; D005260:Female; D007150:Immunohistochemistry; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000187:drug effects / Q000378:metabolism*; D009924:Organ Culture Techniques; D011188:Potassium / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*
chemical_list: D012701:Serotonin; D011188:Potassium