title: Automated large-scale purification of a recombinant g-protein-coupled neurotensin receptor.
abstract: Structure determination of G-protein-coupled receptors and other applications, such as nuclear magnetic resonance (NMR) studies, require milligram quantities of purified, functional receptor protein on a regular basis. This unit presents a step-by-step procedure for the automated two-column purification at the 10-milligram scale of a G protein-coupled receptor for neurotensin, expressed in functional form in Escherichia coli.
authors.affiliation: NIDDK, NIH, Bethesda, Maryland, USA.
authors.forename: Jim F
authors.initials: JF
authors.lastname: White
authors.forename: Reinhard
authors.initials: R
authors.lastname: Grisshammer
mesh_terms: D001331:Automation; D002846:Chromatography, Affinity; D043562:Receptors, G-Protein-Coupled / Q000302:isolation & purification*; D018028:Receptors, Neurotensin / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000302:isolation & purification; D012995:Solubility
chemical_list: D043562:Receptors, G-Protein-Coupled; D018028:Receptors, Neurotensin; D011994:Recombinant Proteins