Solid-phase synthesis of 2'-deoxy-2'-fluoro- beta-D-oligoarabinonucleotides (2'F-ANA) and their phosphorothioate derivatives.
This unit describes the chemical synthesis of 2'-deoxy-2'-fluoro-b-D-oligoarabinonucleotides (2'F-ANA), both with phosphodiester and phosphorothioate linkages. The protocols described herein include araF phosphoramidite preparation, assembly on DNA synthesizers, and final deprotection and purification of oligonucleotides.
Viazovkina
Ekaterina
E
McGill University, Montreal, Canada.
Mangos
Maria M
MM
Elzagheid
Mohamed I
MI
Damha
Masad J
MJ
D001088:Arabinonucleotides / Q000138:chemical synthesis*; D001089:Arabinose / Q000031:analogs & derivatives; D009841:Oligonucleotides / Q000138:chemical synthesis*; D054735:Phosphorothioate Oligonucleotides / Q000138:chemical synthesis*
C118537:2'-deoxy-2'-fluoroarabinose; D001088:Arabinonucleotides; D009841:Oligonucleotides; D054735:Phosphorothioate Oligonucleotides; D001089:Arabinose