RAC1 activity and intracellular ROS modulate the migratory potential of MCF-7 cells through a NADPH oxidase and NFkappaB-dependent mechanism.
In the present study, we demonstrated that changes in Rac1 activity associated with the production of intracellular ROS modulate the migratory properties in MCF-7 and T47D human mammary cell lines. We also described that the NFkappaB pathway exerts a downstream control on the expression of the ROS-dependent cellular migratory potential. These results emphasize the importance of redox balance in the acquisition of malignancy and support previous data sustaining that an oxidative environment predisposes cells to activate signal-transduction pathways actively involved in cellular oncogenesis. Our data also provides evidence that NADPH oxidase could constitute the main source of intracellular ROS in response to changes in Rac1 activity. We suggest that Rac1 plays a role in cellular migration not only limited to its known function in reorganization of the actin cytoskeleton, but also as part of the intracellular machinery that controls the redox balance.
Tobar
Nicolás
N
Laboratorio de Biología Celular, INTA, Universidad de Chile, Casilla 138, Santiago 11, Chile.
Cáceres
Mónica
M
Santibáñez
Juan F
JF
Smith
Patricio C
PC
Martínez
Jorge
J
D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects*; D006801:Humans; D019255:NADPH Oxidases / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D015398:Signal Transduction; D020830:rac1 GTP-Binding Protein / Q000378:metabolism*
D016328:NF-kappa B; D017382:Reactive Oxygen Species; D019255:NADPH Oxidases; D020830:rac1 GTP-Binding Protein