title: Use of monoclonal antibodies for expression cloning.
abstract: This unit details the use of transient expression in mammalian cells to screen cDNA libraries with monoclonal antibodies (MAb) to isolate cDNA clones encoding cell-surface and intracellular proteins. The first protocol describes the cloning of cDNAs encoding cell-surface antigens. The second protocol is a modification that facilitates isolation of cDNAs encoding antigens that are expressed intracellularly. Both protocols are designed for use with the expression vector CDM8, which contains a polylinker for subcloning double-stranded cDNA.
authors.affiliation: Bristol-Myers Squibb, Seattle, Washington, USA.
authors.forename: D
authors.initials: D
authors.lastname: Hollenbaugh
authors.forename: A
authors.initials: A
authors.lastname: Aruffo
authors.forename: B
authors.initials: B
authors.lastname: Jones
authors.forename: P
authors.initials: P
authors.lastname: Linsley
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D000941:Antigens / Q000235:genetics*; D003001:Cloning, Molecular / Q000379:methods*; D018076:DNA, Complementary / Q000235:genetics*; D015723:Gene Library; D005822:Genetic Vectors; D006801:Humans
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens; D018076:DNA, Complementary