Protein identification using a quadrupole ion trap mass spectrometer and SEQUEST database matching.
A procedure is described for rapid, sensitive protein identification utilizing liquid chromatography--tandem mass spectrometry. The analysis is performed on mixtures of peptides obtained by enzyme digestion. The SEQUEST computer program is used to match the sequence information in the spectra to a database of known protein sequences.
Moore
R E
RE
Beckman Research Institute of the City of Hope, Duarte, California, USA.
Young
M K
MK
Lee
T D
TD
D002853:Chromatography, Liquid / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D015203:Reproducibility of Results; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods*
D011506:Proteins