title: Derivatization of glass and polypropylene surfaces.
abstract: This unit describes the derivatization of solid support media, particularly glass and polypropylene, with a linker system by an iterative process that forms a dendrimeric structure, thereby increasing stepwise the loading capacity of the surface. The procedure permits the production of various types of linkers whose characteristics can be tailored to the requirements of the eventual applications. The specific modifications described here are used either for attaching prefabricated DNA oligonucleotides, PCR products, and peptide nucleic acid (PNA) oligomers, or for in situ synthesis of DNA microarrays.
authors.affiliation: Febit AG, Mannheim, Germany.
authors.forename: Markus
authors.initials: M
authors.lastname: Beier
authors.forename: Jörg D
authors.initials: JD
authors.lastname: Hoheisel
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis*; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology; D005898:Glass / Q000737:chemistry*; D008954:Models, Biological; D009696:Nucleic Acids / Q000737:chemistry / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011126:Polypropylenes / Q000737:chemistry*; D011786:Quality Control; D013499:Surface Properties
chemical_list: D020099:Coated Materials, Biocompatible; D003432:Cross-Linking Reagents; D009696:Nucleic Acids; D011126:Polypropylenes; D017438:Bisphenol A-Glycidyl Methacrylate; C033930:Silar (3M)