A TaqI PCR-RFLP detecting a novel SNP in exon 2 of the bovine POU1F1 gene.
PCR-SSCP and DNA sequencing methods were applied to reveal three novel single nucleotide polymorphisms (SNPs) in exon 2 of the POU1F1 gene in 963 Chinese cattle belonging to eight breeds. Among them, a silent SNP (NM_174579:c.545G > A) detected by TaqI endonuclease is described. Frequencies of the POU1F1-G allele varied from 0.685 to 1.000. The association of TaqI polymorphism with growth traits was analyzed in 251 Nanyang cattle. No significant associations of the TaqI polymorphism with body weight and average daily gain for different growth periods (6, 12, 18, and 24 months old) were observed (P > 0.05), as well as for body sizes (P > 0.05).
Pan
Chuanying
C
Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China.
Lan
Xianyong
X
Chen
Hong
H
Guo
Yikun
Y
Shu
Jianhong
J
Lei
Chuzhao
C
Wang
Xinzhuang
X
D000818:Animals; D001483:Base Sequence; D002417:Cattle / Q000235:genetics* / Q000254:growth & development; D005091:Exons; D005787:Gene Frequency; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D019914:Taq Polymerase; D050817:Transcription Factor Pit-1 / Q000235:genetics*
D050817:Transcription Factor Pit-1; D019914:Taq Polymerase