Transfection of lymphoid cells.
Using available vectors, a single immunoglobulin chain or both heavy (H) and light (L) chains can be simultaneously transferred to recipient myeloma cells. Several methods can be used to prepare stable transfected lymphoid cells. The Basic Protocol in this unit describes protoplast fusion, which is suitable for certain lymphoid cells but tends to be more cumbersome than electroporation or liposome-mediated transfection, which are described in the two alternate protocols. All three techniques may be adapted for stable transfection of other eukaryotic cell lines.
Morrison
S L
SL
University of California Los Angeles, Los Angeles, California, USA.
D000818:Animals; D002459:Cell Fusion / Q000379:methods; D045744:Cell Line, Tumor; D018274:Electroporation / Q000379:methods; D004926:Escherichia coli / Q000166:cytology / Q000235:genetics; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000096:biosynthesis* / Q000235:genetics; D007147:Immunoglobulin Light Chains / Q000096:biosynthesis* / Q000235:genetics; D008081:Liposomes / Q000737:chemistry / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism*; D008545:Melanoma / Q000235:genetics / Q000378:metabolism; D011523:Protoplasts / Q000166:cytology / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D008081:Liposomes