Solid-phase oligonucleotide labeling with DOTA.
This unit describes a method to construct oligonucleotide conjugates labeled with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) on a solid support. A nucleosidic phosphoramidite that contains a protected DOTA ligand compatible with normal chain assembly is prepared first. As the chain assembly is completed, the oligonucleotide is deprotected and converted to the corresponding gadolinium(III) chelate, resulting in an oligonucleotide conjugate containing the chelate at the 5'-terminus.
Jaakkola
Lassi
L
PerkinElmer Life and Analytical Sciences, Turku, Finland.
Ylikoski
Alice
A
Hovinen
Jari
J
D000577:Amides / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
D000577:Amides; D006573:Heterocyclic Compounds, 1-Ring; D009841:Oligonucleotides; D010756:Phosphoric Acids; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; C011067:phosphoramidic acid