Production of anti-peptide antisera.
The protocols described in this unit can be used for generating and testing anti-peptide antisera, assuming that a scientist has already selected and conjugated peptides for experimental vaccines. Described are immunization and bleeding of experimental animals, a simple ELISA for antiserum screening, and affinity purification of the serum, including preparation of columns.
Carter
John Mark
JM
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007106:Immune Sera / Q000276:immunology* / Q000302:isolation & purification*; D007114:Immunization / Q000379:methods*; D010455:Peptides / Q000276:immunology* / Q000494:pharmacology; D014614:Vaccines, Synthetic / Q000276:immunology* / Q000494:pharmacology
D007106:Immune Sera; D010455:Peptides; D014614:Vaccines, Synthetic