DNA synthesis without base protection using the phosphoramidite approach.
In the strategy of DNA synthesis without base protection, a recent O-selective phosphorylation procedure known as the "activated phosphite method" is used to generate reactive phosphite intermediates from N-unprotected phosphoramidite building blocks. This new strategy proved to be advantageous over previous strategies because the cleavage of the N-P(III) bond, which can be formed by the significant phosphitylation of the nucleobase amino groups, can be omitted. The activated phosphite method provides an attractive route to high-throughput synthesis of DNA and the synthesis of alkali-labile modified DNAs such as oligonucleotides containing DNA lesions. This unit presents two convenient methods for the synthesis of N-unprotected deoxynucleoside 3'-phosphoramidite units and detailed procedures for automated synthesis of oligodeoxyribonucleotides from these phosphoramidite units.
Ohkubo
Akihiro
A
Tokyo Institute of Technology, Tokyo, Japan.
Seio
Kohji
K
Sekine
Mitsuo
M
D000577:Amides / Q000737:chemistry*; D004247:DNA / Q000138:chemical synthesis*; D009682:Magnetic Resonance Spectroscopy; D010756:Phosphoric Acids / Q000737:chemistry*; D010766:Phosphorylation; D021241:Spectrometry, Mass, Electrospray Ionization
D000577:Amides; D010756:Phosphoric Acids; D004247:DNA; C011067:phosphoramidic acid