CD24 cross-linking induces apoptosis in, and inhibits migration of, MCF-7 breast cancer cells.
BACKGROUND
The biological effects of CD24 (FL-80) cross-linking on breast cancer cells have not yet been established. We examined the impact of CD24 cross-linking on human breast cancer cell line MCF-7.


METHODS
MCF-7 and MDA-MB-231 cells were treated with anti-rabbit polyclonal IgG or anti-human CD24 rabbit polyclonal antibodies to induce cross-linking, and then growth was studied. Changes in cell characteristics such as cell cycle modulation, cell death, survival in three-dimensional cultures, adhesion, and migration ability were assayed after CD24 cross-linking in MCF-7.


RESULTS
Expression of CD24 was analyzed by flow cytometry in MDA-MB-231 and MCF-7 cells where 2% and 66% expression frequencies were observed, respectively. CD24 cross-linking resulted in time-dependent proliferation reduction in MCF-7 cells, but no reduction in MDA-MB-231 cells. MCF-7 cell survival was reduced by 15% in three-dimensional culture after CD24 cross-linking. Increased MCF-7 cell apoptosis was observed after CD24 cross-linking, but no cell cycle arrest was observed in that condition. The migration capacity of MCF-7 cells was diminished by 30% after CD24 cross-linking.


CONCLUSIONS
Our results showed that CD24 cross-linking induced apoptosis and inhibited migration in MCF-7 breast cancer cells. We conclude that CD24 may be considered as a novel therapeutic target for breast cancer.
Kim
Jong Bin
JB
Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea. kkimjp@hanmail.net
Ko
Eunyoung
E
Han
Wonshik
W
Lee
Jeong Eon
JE
Lee
Kyung-Min
KM
Shin
Incheol
I
Kim
Sangmin
S
Lee
Jong Won
JW
Cho
Jihyoung
J
Bae
Ji-Yeon
JY
Jee
Hyeon-Gun
HG
Noh
Dong-Young
DY
D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy; D051927:CD24 Antigen / Q000276:immunology*; D045744:Cell Line, Tumor; D003165:Complement System Proteins / Q000276:immunology* / Q000378:metabolism; D025461:Feedback, Physiological; D005260:Female; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism / Q000627:therapeutic use; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011817:Rabbits; D011961:Receptors, Fc / Q000378:metabolism; D015398:Signal Transduction
D051927:CD24 Antigen; D007141:Immunoglobulin Fc Fragments; D011961:Receptors, Fc; D003165:Complement System Proteins