title: Novel composite hyaluronan/type I collagen/fibrin scaffold enhances repair of osteochondral defect in rabbit knee.
abstract: A new composite scaffold containing type I collagen, hyaluronan, and fibrin was prepared with and without autologous chondrocytes and implanted into a rabbit femoral trochlea. The biophysical properties of the composite scaffold were similar to native cartilage. The macroscopic, histological, and immunohistochemical analysis of the regenerated tissue from cell-seeded scaffolds was performed 6 weeks after the implantation and predominantly showed formation of hyaline cartilage accompanied by production of glycosaminoglycans and type II collagen with minor fibro-cartilage production. Implanted scaffolds without cells healed predominantly as fibro-cartilage, although glycosaminoglycans and type II collagen, which form hyaline cartilage, were also observed. On the other hand, fibro-cartilage or fibrous tissue or both were only formed in the defects without scaffold. The new composite scaffold containing collagen type I, hyaluronan, and fibrin, seeded with autologous chondrocytes and implanted into rabbit femoral trochlea, was found to be highly effective in cartilage repair after only 6 weeks. The new composite scaffold can therefore enhance cartilage regeneration of osteochondral defects, by the supporting of the hyaline cartilage formation.
authors.affiliation: Department of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. evafil@biomed.cas.cz
authors.forename: Eva
authors.initials: E
authors.lastname: Filová
authors.forename: Frantisek
authors.initials: F
authors.lastname: Jelínek
authors.forename: Milan
authors.initials: M
authors.lastname: Handl
authors.forename: Andriy
authors.initials: A
authors.lastname: Lytvynets
authors.forename: Michala
authors.initials: M
authors.lastname: Rampichová
authors.forename: Ferdinand
authors.initials: F
authors.lastname: Varga
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Cinátl
authors.forename: Tomás
authors.initials: T
authors.lastname: Soukup
authors.forename: Tomás
authors.initials: T
authors.lastname: Trc
authors.forename: Evzen
authors.initials: E
authors.lastname: Amler
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D024042:Collagen Type I; D005337:Fibrin; D006820:Hyaluronic Acid; D007718:Knee Injuries / Q000601:surgery*; D008297:Male; D008422:Materials Testing; D008854:Microscopy, Electron; D019736:Prostheses and Implants; D011817:Rabbits
chemical_list: D024042:Collagen Type I; D005337:Fibrin; D006820:Hyaluronic Acid