title: Kinetic assay methods.
abstract: The purpose of this unit is to provide a brief review of issues important in the design of initial-rate assay methods. General aspects of kinetic assay design are discussed, including enzyme and substrate purity, concentration and stability. Also covered are issues such as continuous versus stop-time assay formats, coupled enzyme assays, binding studies, and presentation of initial-rate data.
authors.affiliation: University of Florida, Gainesville, Florida, USA.
authors.forename: R D
authors.initials: RD
authors.lastname: Allison
mesh_terms: D002384:Catalysis; D004798:Enzymes / Q000378:metabolism*; D007700:Kinetics; D011485:Protein Binding; D013379:Substrate Specificity
chemical_list: D004798:Enzymes