title: A high-density intervarietal map of the wheat genome enriched with markers derived from expressed sequence tags.
abstract: Bread wheat (Triticum aestivum L.) is a hexaploid species with a large and complex genome. A reference genetic marker map, namely the International Triticeae Mapping Initiative (ITMI) map, has been constructed with the recombinant inbred line population derived from a cross involving a synthetic line. But it is not sufficient for a full understanding of the wheat genome under artificial selection without comparing it with intervarietal maps. Using an intervarietal mapping population derived by crossing Nanda2419 and Wangshuibai, we constructed a high-density genetic map of wheat. The total map length was 4,223.1 cM, comprising 887 loci, 345 of which were detected by markers derived from expressed sequence tags (ESTs). Two-thirds of the high marker density blocks were present in interstitial and telomeric regions. The map covered, mostly with the EST-derived markers, approximately 158 cM of telomeric regions absent in the ITMI map. The regions of low marker density were largely conserved among cultivars and between homoeologous subgenomes. The loci showing skewed segregation displayed a clustered distribution along chromosomes and some of the segregation distortion regions (SDR) are conserved in different mapping populations. This map enriched with EST-derived markers is important for structure and function analysis of wheat genome as well as in wheat gene mapping, cloning, and breeding programs.
authors.affiliation: The Applied Plant Genomics Laboratory, Crop Genomics and Bioinformatics Centre, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China.
authors.forename: Shulin
authors.initials: S
authors.lastname: Xue
authors.forename: Zhengzhi
authors.initials: Z
authors.lastname: Zhang
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.forename: Zhongxin
authors.initials: Z
authors.lastname: Kong
authors.forename: Yong
authors.initials: Y
authors.lastname: Cao
authors.forename: Chunjun
authors.initials: C
authors.lastname: Li
authors.forename: Hongying
authors.initials: H
authors.lastname: Yi
authors.forename: Mingfeng
authors.initials: M
authors.lastname: Mei
authors.forename: Huilan
authors.initials: H
authors.lastname: Zhu
authors.forename: Jizhong
authors.initials: J
authors.lastname: Wu
authors.forename: Haibin
authors.initials: H
authors.lastname: Xu
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhao
authors.forename: Dagang
authors.initials: D
authors.lastname: Tian
authors.forename: Caiqin
authors.initials: C
authors.lastname: Zhang
authors.forename: Zhengqiang
authors.initials: Z
authors.lastname: Ma
mesh_terms: D002874:Chromosome Mapping; D020090:Chromosome Segregation; D032461:Chromosomes, Plant / Q000378:metabolism; D018744:DNA, Plant / Q000378:metabolism; D020224:Expressed Sequence Tags; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics*; D011110:Polymorphism, Genetic; D014908:Triticum / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers