Hepatoprotective activity of Sapindus mukorossi and Rheum emodi extracts: in vitro and in vivo studies.
OBJECTIVE
To study the hepatoprotective capacity of Sapindus mukorossi (S. mukorossi) and Rheum emodi (R. emodi) extracts in CCl(4) treated male rats.


METHODS
The dried powder of S. mukorossi and R. emodi was extracted successively with petroleum ether, benzene, chloroform, and ethanol and concentrated in vacuum. Primary rat hepatocyte monolayer cultures were used for in vitro studies. In vivo, the hepatoprotective capacity of the extract of the fruit pericarp of S. mukorossi and the rhizomes of R. emodi was analyzed in liver injured CCl(4)-treated male rats.


RESULTS
In vitro: primary hepatocytes monolayer cultures were treated with CCl(4) and extracts of S. mukorossi & R. emodi. A protective activity could be demonstrated in the CCl(4) damaged primary monolayer culture. In vivo: extracts of the fruit pericarp of S. mukorossi (2.5 mg/mL) and rhizomes of R. emodi (3.0 mg/mL) were found to have protective properties in rats with CCl(4) induced liver damage as judged from serum marker enzyme activities.


CONCLUSIONS
The extracts of S. mukorossi and R. emodi do have a protective capacity both in vitro on primary hepatocytes cultures and in in vivo in a rat model of CCl(4) mediated liver injury.
Ibrahim
Mohammed
M
Centre for Liver Research and Diagnostics, Deccan college of Medical Sciences and Allied Hospitals, Kanchanbagh, Hyderabad 500058, Andhra Pradesh, India. ibrahim_cce@rediffmail.com
Khaja
Mohammed Nane
MN
Aara
Anjum
A
Khan
Aleem Ahmed
AA
Habeeb
Mohammed Aejaz
MA
Devi
Yalavarthy Prameela
YP
Narasu
Mangamoori Lakshmi
ML
Habibullah
Chitoor Mohammed
CM
D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects / Q000473:pathology / Q000502:physiology*; D007770:L-Lactate Dehydrogenase / Q000032:analysis; D008099:Liver / Q000187:drug effects / Q000502:physiology*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use*; D020011:Protective Agents / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012250:Rheum; D032127:Sapindus; D017855:Transferases (Other Substituted Phosphate Groups) / Q000032:analysis
D010936:Plant Extracts; D020011:Protective Agents; D002251:Carbon Tetrachloride; D007770:L-Lactate Dehydrogenase; D017855:Transferases (Other Substituted Phosphate Groups); C106504:UDP-GlcNAc-undecaprenyl phosphate N-acetylglucosaminyl 1-phosphate transferase