Genetic analyses on DNA microarrays.
A great stir has coursed through the genetics community with the advent in 1996 of "chip technologies," a series of related techniques that allow DNA hybridization-based studies to be performed with unprecedented speed and parallelism. All chip technologies have in common a microarray, a small glass chip approximately one square centimeter in area, to which nucleotide sequences are bound. Fluorescently labeled nucleic acids are hybridized to the microarray and imaged with a laser scanner or fluorescence microscope. This unit offers an overview of the two dominant technologies, cDNA microarrays and oligonucleotide chips. It concludes with a discussion regarding how well microarrays perform real-world expression analysis.
Stein
L D
LD
Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling / Q000379:methods; D005826:Genetics, Medical; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D009841:Oligonucleotides / Q000235:genetics
D018076:DNA, Complementary; D009841:Oligonucleotides