title: Basophil histamine release activity and disease severity in chronic idiopathic urticaria.
abstract: BACKGROUND
  Altered basophil degranulation phenotypes are found in patients with chronic idiopathic urticaria (CIU).
  
  
  OBJECTIVE
  To evaluate CIU disease severity in relation to basophil histamine release (HR) characteristics.
  
  
  METHODS
  Patients with CIU were recruited from allergy and dermatology clinics. Patients with recent use of systemic corticosteroids or immunosuppressants were excluded. Patients completed disease severity surveys and had blood basophils isolated and stimulated for HR using polyclonal goat anti-human IgE and N-formyl-met-leu-phe. The HR was measured using automated fluorometry. Multivariate linear regression analyses were used to investigate relationships between HR data and CIU disease measures.
  
  
  RESULTS
  Fifty patients completed surveys, of which 34 were further categorized into 2 subgroups based on basophil HR response to anti-IgE stimulation: responders (> or = 10% HR) and nonresponders (< 10% HR). Responders and nonresponders reported similar use of oral corticosteroids, work absences, and quality-of-life impairment but differed in their patterns of medications used for CIU. Basophil responders had a trend of higher use of the emergency department for CIU management. Multivariate regression revealed that patients with the basophil responder phenotype experienced significantly higher current itch scores (P = .02) compared with nonresponders.
  
  
  CONCLUSIONS
  Quality-of-life impairment is similar in CIU basophil subsets. Patients with CIU with a basophil responder phenotype report longer disease duration, a higher frequency of emergency department use, and significantly higher itch severity.
authors.affiliation: Division of Allergy and Clinical Immunology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: Rosalyn
authors.initials: R
authors.lastname: Baker
authors.forename: Kavitha
authors.initials: K
authors.lastname: Vasagar
authors.forename: Nkiruka
authors.initials: N
authors.lastname: Ohameje
authors.forename: Laura
authors.initials: L
authors.lastname: Gober
authors.forename: Suephy C
authors.initials: SC
authors.lastname: Chen
authors.forename: Patricia M
authors.initials: PM
authors.lastname: Sterba
authors.forename: Sarbjit S
authors.initials: SS
authors.lastname: Saini
mesh_terms: D000328:Adult; D001491:Basophils / Q000378:metabolism*; D002908:Chronic Disease; D005260:Female; D006632:Histamine / Q000097:blood; D006636:Histamine Release; D006801:Humans; D008297:Male; D008875:Middle Aged; D014581:Urticaria / Q000503:physiopathology*
chemical_list: D006632:Histamine