Pituitary toxicity but lack of rat colon carcinogenicity of a DC-magnetic field in a medium-term bioassay.
The present study was designated to evaluate the effect of direct current induced permanent magnetic field (DC-MF) on chemically induced rat colon carcinogenesis. Five experimental groups of male S.D. rats were injected with 1,2-dimethylhydrazine (DMH) subcutaneously, 20 mg/kg b.wt., once a week for four weeks, with exposure to 1 mT DC-MF (12 hours/day) as follows: Before (pre) the carcinogen administration (group 1), simultaneously (group 2), after (post) the carcinogen administration (group 3) and daily from the beginning to the end of the experiment after 12 weeks (group 4). Rats of group 5 served as carcinogen-only treated controls while those of group 6 were non-treated controls. There were no differences in the incidences and multiplicities of colonic aberrant crypt foci (ACF), putative preneoplastic lesions, among all groups except that large foci in group 1 were significantly fewer in numbers than those found in group 5. Proliferating cell nuclear antigen labeling indexes (PCNA-LI) in the colon epithelium were essentially the same in MF-treated and control rats. Histopathological examination showed evident hemorrhage in the pituitary glands of some rats of groups 1-3, and in most rats of group 4. Transmission electron microscopy also revealed ultrastructural changes, but DNA ploidy analysis revealed no carcinogenicity to MF-exposed pituitary glands. Serum levels of AST, ALT, total protein, creatinine, albumin, albumin/globulin ratio and growth hormone levels did not change among the groups. The present study revealed that the action of an artificial MF on rats is not carcinogenic/or cancer-promoting, at least in the present protocol for colon carcinogenesis.
Salim
Elsayed I
EI
Zoology Department, Faculty of Science, Tanta University, Tanta 31527, Egypt. elsalem_777@yahoo.com
Omar
Khalid M
KM
Abou-Hattab
Hassan A
HA
Abou-Zaid
Fouad A
FA
D019813:1,2-Dimethylhydrazine / Q000633:toxicity; D000818:Animals; D001681:Biological Assay; D001774:Blood Chemical Analysis; D001835:Body Weight; D002273:Carcinogens / Q000633:toxicity; D003106:Colon / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D003110:Colonic Neoplasms / Q000209:etiology / Q000473:pathology*; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004560:Electricity / Q000009:adverse effects*; D007124:Immunoenzyme Techniques; D008280:Magnetics; D008297:Male; D009929:Organ Size; D010902:Pituitary Gland / Q000187:drug effects / Q000528:radiation effects*; D011003:Ploidies; D011230:Precancerous Conditions / Q000209:etiology / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen; D051381:Rats; D017207:Rats, Sprague-Dawley
D002273:Carcinogens; D018809:Proliferating Cell Nuclear Antigen; D004247:DNA; D019813:1,2-Dimethylhydrazine