title: Mechanism of calcium hydroxide-induced neutrophil migration into air-pouch cavity.
abstract: The aim of this study was to investigate cellular migration induced by calcium hydroxide to air-pouch cavities in mice. The migration was more specific to neutrophil and was dose and time dependent (peaking 96 h after stimulation). This migration was inhibited by pretreatment with thalidomide, indomethacin, MK886, meloxicam, dexamethasone, MK886 associated with indomethacin, and MK886 associated with indomethacin and dexamethasone. The air-pouch exudate from animals stimulated with calcium hydroxide showed an increase of leukotriene-B(4) (LTB(4)), interleukin-1beta, tumor necrosis factor alpha (TNF-alpha), cytokine-induced neutrophil chemoattractant (KC), and macrophage inflammatory protein 2 (MIP-2) release. Pretreatment with 3% thioglycollate increased the macrophage population in the air pouch but did not change neutrophil migration. Depleting the resident mast cells through chronic pretreatment with compound 48/80 did not alter neutrophil migration in response to calcium hydroxide. It was possible to conclude that calcium hydroxide-induced neutrophil migration to the air-pouch cavity in mice is mediated by LTB(4), TNF-alpha, KC, MIP-2, and prostaglandins, but it was not dependent on macrophages or mast cells.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry, Sao Paulo State University, Araçatuba, Brazil. moraes_mari@hotmail.com <moraes_mari@hotmail.com>
authors.forename: Mariana Machado Teixeira de Moraes
authors.initials: MM
authors.lastname: Costa
authors.forename: Sandra Helena Penha
authors.initials: SH
authors.lastname: de Oliveira
authors.forename: João Eduardo
authors.initials: JE
authors.lastname: Gomes-Filho
mesh_terms: D000388:Air; D000818:Animals; D002126:Calcium Hydroxide / Q000009:adverse effects*; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020556:Neutrophil Infiltration / Q000502:physiology*; D011453:Prostaglandins / Q000378:metabolism; D012387:Root Canal Filling Materials / Q000009:adverse effects*; D040521:Subcutaneous Tissue
chemical_list: D016207:Cytokines; D011453:Prostaglandins; D012387:Root Canal Filling Materials; D002126:Calcium Hydroxide