title: [Inhomogeneous expression of fusion protein HSA/IL1ra in Pichia pastoris].
abstract: To investigate the causes and influencing factors of heterogeneity of HSA/IL1ra fusion protein expression in Pichia pastoris.
  
  
  
  The heterogeneity of HSA/IL1ra fusion protein expressed in Pichia pastoris was studied by removing glycosylation and inhibiting glycosylation, as well as by different ways of fusion, different clones, and different expression host.
  
  
  
  Glycosylation caused expression heterogeneity of fusion protein, but in SMD1168 and some GS115 clones there was no this phenomenon.
  
  
  
  The expression heterogeneity of HSA/IL1ra fusion protein in Pichia pastoris is due to the glycosylation, and different ways of fusion, different clones, different expression host also have some impact.
authors.affiliation: Institution of Pharmacology & Toxicology and Biological Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Shou-feng
authors.initials: SF
authors.lastname: Dai
authors.forename: Qi
authors.initials: Q
authors.lastname: Shen
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Shu-qing
authors.initials: SQ
authors.lastname: Chen
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018740:Genetic Heterogeneity; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000096:biosynthesis* / Q000235:genetics; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D012709:Serum Albumin / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D011993:Recombinant Fusion Proteins; D012709:Serum Albumin