title: Changes in Clara cell 10 kDa protein (CC10)-positive cell distribution in acute lung injury following repeated lipopolysaccharide challenge in the rat.
abstract: Clara cell 10 kDa protein (CC10) is the major secretory protein of Clara cells and is thought to play a protective role in the lung owing to its anti-inflammatory properties. There is little information on the anatomical distribution of CC10-positive cells in rat lung following lipopolysaccharide (LPS) challenge. We have determined the expression of CC10 along the tracheobronchial tree in saline-treated and LPS-treated rats. Saline-treated rats showed sporadic CC10 staining in central airways and abundant staining in bronchioles. In transitional airways, most cells were positive except for squamous cells. Following LPS challenge, there was a reduction in staining in the upper airways but little change within bronchioles. Squamous epithelia within the transitional airways now showed positive staining. These cells also co-stained for pancytokeratin and appeared to co-localize with surfactant D- and Ki67-positive cells, indicating the presence of a dedifferentiated cell type with both epithelial and pneumocyte phenotypes. These data show that diffuse inflammatory injury results in generalized loss of CC10 in central airways. Conversely, the transitional airways showed evidence of a dedifferentiated population of squamous cells that now stained for CC10. We hypothesize that this is an attempt by peripheral lung to maintain alveolar sac integrity during an inflammatory episode.
authors.affiliation: Department of Pathology, Safety Assessment UK, AstraZeneca R&D Charnwood, Loughborough, Leicestershire LE11 5RH, United Kingdom. Sarahj.Bolton@astrazeneca.com
authors.forename: S J
authors.initials: SJ
authors.lastname: Bolton
authors.forename: K
authors.initials: K
authors.lastname: Pinnion
authors.forename: C V
authors.initials: CV
authors.lastname: Marshall
authors.forename: E
authors.initials: E
authors.lastname: Wilson
authors.forename: J E
authors.initials: JE
authors.lastname: Barker
authors.forename: V
authors.initials: V
authors.lastname: Oreffo
authors.forename: M L
authors.initials: ML
authors.lastname: Foster
mesh_terms: D000208:Acute Disease; D000280:Administration, Inhalation; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001980:Bronchi / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004791:Enzyme Inhibitors / Q000378:metabolism*; D007633:Keratins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008171:Lung Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology; D008297:Male; D037663:Pulmonary Surfactant-Associated Protein D / Q000378:metabolism; D051381:Rats; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D014598:Uteroglobin / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D004791:Enzyme Inhibitors; D019394:Ki-67 Antigen; D008070:Lipopolysaccharides; D037663:Pulmonary Surfactant-Associated Protein D; C556391:Scgb1a1 protein, rat; D007633:Keratins; D014598:Uteroglobin