title: Evaluation of two incubation environments--ISO class 8 versus ISO class 5--on intracytoplasmic sperm injection cycle outcome.
abstract: OBJECTIVE
  To evaluate the impact of two different incubation environments-class 8 versus class 5-on embryo quality and pregnancy rate.
  
  
  METHODS
  Retrospective comparative study.
  
  
  METHODS
  Private fertility and gynecology clinic.
  
  
  METHODS
  123 consecutive intracytoplasmic sperm injection (ICSI) cycles were analyzed from January 2002 to February 2005. Cycles were divided into two groups: in group I (n = 60), the embryo culture was performed in class 8 air quality; in group II (n = 63), the embryo culture was performed in class 5 air quality.
  
  
  METHODS
  None.
  
  
  METHODS
  Number of embryos available for transfer, number of good quality embryos transferred, implantation rate, and clinical pregnancy.
  
  
  RESULTS
  Age of women, duration of stimulation, total doses of gonadotropins, endometrial thickness on the day of human chorionic gonadotropin (hCG) administration, number of oocytes metaphase II retrieved, number of embryos available, number of good quality embryos transferred, fertilization and cleavage rates, implantation rate, and clinical pregnancy were not statistically different between the groups.
  
  
  CONCLUSIONS
  Our study demonstrated that incubation environment class 8 is as good as incubation environment class 5 when compared in relation to the parameters analyzed. We believe that there is still room for improvement in the overall outcome of ICSI embryos.
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Ginecologia, Rio de Janeiro, Brazil. mariadocarmo@cmb.com.br
authors.forename: Maria do Carmo B
authors.initials: Mdo C
authors.lastname: Souza
authors.forename: Ana Cristina A
authors.initials: AC
authors.lastname: Mancebo
authors.forename: Christina de A
authors.initials: Cde A
authors.lastname: da Rocha
authors.forename: Carlos André
authors.initials: CA
authors.lastname: Henriques
authors.forename: Marcelo M
authors.initials: MM
authors.lastname: Souza
authors.forename: Fernanda F O
authors.initials: FF
authors.lastname: Cardoso
mesh_terms: D000328:Adult; D016902:Air Pollution, Indoor; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D011247:Pregnancy; D018873:Pregnancy Rate; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*