title: Quantification of cysteine S-conjugate of 3-sulfanylhexan-1-ol in must and wine of petite arvine vine by stable isotope dilution analysis.
abstract: Making use of a convenient synthetic approach to prepare the deuterated S-3-(hexan-1-ol)-cysteine by a Michael addition reaction, an analytical method was developed to measure the presence of the cysteine S-conjugate, precursor of 3-sulfanylhexan-1-ol (3-mercaptohexan-1-ol), in must and wine from Petite Arvine vine. The method uses a stable isotope dilution assay with a suitable one-step sample preparation and HPLC-MS detection. The method has limits of detection and quantification of 3 and 10 microg/L, respectively. A correlation between the increase of the precursor concentration and the increase of the degree of rot has been established.
authors.affiliation: Institute of Life Technologies, University of Applied Sciences Western Switzerland Valais, Route de Rawyl 64, CH-1950 Sion 2, Switzerland.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Luisier
authors.forename: Hermann
authors.initials: H
authors.lastname: Buettner
authors.forename: Sebastian
authors.initials: S
authors.lastname: Völker
authors.forename: Thierry
authors.initials: T
authors.lastname: Rausis
authors.forename: Urban
authors.initials: U
authors.lastname: Frey
mesh_terms: D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D003903:Deuterium; D005285:Fermentation; D005638:Fruit / Q000737:chemistry*; D000441:Hexanols / Q000032:analysis* / Q000378:metabolism; D007201:Indicator Dilution Techniques; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
chemical_list: D000441:Hexanols; C528100:S-3-(hexan-1-ol)cysteine; D013438:Sulfhydryl Compounds; D003903:Deuterium; D003545:Cysteine; C477019:3-mercaptohexanol