Biotinylation and analysis of membrane-bound and soluble proteins.
Coupling of a biotin moiety to proteins allows sensitive detection of those proteins with a variety of commercially available avidin or streptavidin conjugates. This unit presents protocols for biotinylation of cell-surface proteins and proteins in solution. A major advantage of these techniques is that they do not require use of radioisotopes (although use of radioiodinated streptavidin is optional for biotin detection).
Lisanti
M P
MP
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Sargiacomo
M
M
D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012995:Solubility; D019809:Streptavidin / Q000737:chemistry
D008565:Membrane Proteins; D001360:Avidin; D001710:Biotin; D019809:Streptavidin