Redistribution of cytosolic FGFR1 after induced migration of urothelial cells in culture.
The distribution of cytosolic fibroblast growth factor receptor 1 (FGFR1) was studied in correlation to cell migration in urothelial cell line g/G. Cell motility was analysed with a new method using consecutive series of photographs of cells relocated on CELLocate coverslips and with image analysis software. The results confirmed that FGF1 stimulated cell motility only when cells were grown on collagen I coating. During the transition from sessile to motile cell phenotype a complete redistribution of cytosolic FGFR1 was revealed. In sessile cells, FGFR1 had a filamentous distribution and its location matched cytokeratin 7. In cells of the migrating phenotype, the distribution of FGFR1 was diffuse, mainly located in cytosol. Our data reveal that the location of cytosolic FGFR1 depends on the motile characteristics of the cell. The results also indicate that attachment of cells to collagen I is crucial for the induction of urothelial cell motility with FGF1.
Veranic
Peter
P
Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia. peter.veranic@mf.uni-lj.si
Daris
Barbara
B
Zupancic
Dasa
D
Batista
Urska
U
Jezernik
Kristijan
K
D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D002460:Cell Line; D002465:Cell Movement; D003094:Collagen / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism*; D051379:Mice; D051496:Receptor, Fibroblast Growth Factor, Type 1 / Q000378:metabolism*; D019459:Urothelium / Q000166:cytology* / Q000378:metabolism
D003094:Collagen; D051496:Receptor, Fibroblast Growth Factor, Type 1