[Development and application of a two-step MacELISA for the early diagnosis of hemorrhagic fever with renal syndrome].
OBJECTIVE
To develop and improve a MacELISA method for the early diagnosis of hemorrhagic fever with renal syndrome (HFRS) with simplified operation procedure.


METHODS
The nucleic proteins of hantavirus were labeled with horse raddish peroxidase (HRP) and used as detection antigens. A two-step MacELISA based HRP conjugated antigen was established and the detection sensitivity and specificity were compared with commonly used three-step MacELISA.


RESULTS
This method could be used to detect hantanvirus specific IgM with high sensitivity and specificity from human patient serum. There was not significant difference from commonly used three-step MacELISA and the sensitivity and specificity were 100%.


CONCLUSIONS
This method is simple, sensitive and rapid in operation, and therefore could be used for the early diagnosis of HFRS.
Zhang
Quan-fu
QF
State Key Laboratory for Infectious Diseases, Institute for Viral Diseases Control and Prevention, Chinese Center for Disease Prevention and Control Beijing 100052, China.
Li
Jian-dong
JD
Li
Wei-hong
WH
Li
Chuan
C
Liu
Qin-zhi
QZ
Liang
Mi-fang
MF
Li
De-xin
DX
D000818:Animals; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000097:blood / Q000175:diagnosis*; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
D007075:Immunoglobulin M