Separation of cytidine 5'-triphosphate biosynthesized from cytidine 5'-monophosphate on ion-exchange resin and HPLC analysis of cytidine compounds.
Conditions were studied in the biosynthesis of cytidine 5'-triphosphate (CTP) from cytidine 5'-monophosphate (CMP). A 201 x 7 anion ion-exchange resin was applied for the separation of CTP from CMP. Adsorption isotherm and elution conditions (eluant, eluant concentration, flow rate, sample volume loaded) were investigated. At the same time, a new high-performance liquid chromatography on an anion ion-exchange column WAX-1 with UV detector at 260 nm was developed to measure CMP, cytidine 5'-diphosphate (CDP), and CTP. The retention time for CMP, CDP, and CTP are 0.723, 1.448, and 4.432 min, respectively. This new rapid high-performance liquid chromatography (HPLC) method for the analysis of cytidine compounds in biological sample has a wide linear range with high precision and repeatability.
Shi
Lu-E
LE
College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
Ying
Guo-Qing
GQ
Tang
Zhen-Xing
ZX
Yi
Yu
Y
Shan
Jian-Feng
JF
Liu
Hua-Zhang
HZ
D000327:Adsorption; D001709:Biotechnology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003565:Cytidine Diphosphate / Q000032:analysis; D003568:Cytidine Monophosphate / Q000032:analysis / Q000378:metabolism*; D003570:Cytidine Triphosphate / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification*; D007475:Ion Exchange Resins; D012441:Saccharomyces cerevisiae / Q000378:metabolism
D007475:Ion Exchange Resins; D003565:Cytidine Diphosphate; D003570:Cytidine Triphosphate; D003568:Cytidine Monophosphate