Controlled release carrier of BSA made by W/O/W emulsion method containing PLGA and hydroxyapatite.
This study relates to the Bovine Serum Albumin (BSA) protein-controlled release system with Poly(lactic-glycolic acid) (PLGA) biodegradable polymer. It also has special double emulsification carriers containing alkaline material of hydroxyapatite (HAp), which can carry the hydrophilic drug effectively and sustain a controlled substance release. The controlled release strategy is based on the HAp absorption ability, which will be trapped into the core of PLGA microsphere. Besides, the acidic degradation products of PLGA polymer and basic inorganic component of HAp can be used to control the dissolution of microsphere, then resulting in protein release. We have varied the HAp amount to observe its effect on microsphere characteristics such as the particle size, surface/internal morphology, BSA entrapment efficiency, microsphere degradation, BSA in vitro release behaviour, and cell toxicity etc.
Ho
Mei Ling
ML
Department of Physiology, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Fu
Yin Chih
YC
Wang
Gwo Jaw
GJ
Chen
Hui Ting
HT
Chang
Je Ken
JK
Tsai
Tsung Hsien
TH
Wang
Chih Kuang
CK
D000327:Adsorption; D000818:Animals; D041702:BALB 3T3 Cells; D001682:Biological Availability; D002021:Buffers; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology; D017886:Durapatite / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D004655:Emulsions / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D006016:Glycolates / Q000737:chemistry* / Q000494:pharmacology; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry* / Q000494:pharmacology; D008752:Methylene Chloride / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D008863:Microspheres; D010316:Particle Size; D011100:Polyglycolic Acid / Q000737:chemistry* / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011142:Polyvinyl Alcohol / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics; D013499:Surface Properties; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
D002021:Buffers; D004337:Drug Carriers; D004655:Emulsions; C518288:FITC-albumin; D006016:Glycolates; D012709:Serum Albumin; D014867:Water; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D012710:Serum Albumin, Bovine; D019344:Lactic Acid; D008752:Methylene Chloride; D011142:Polyvinyl Alcohol; D017886:Durapatite; D016650:Fluorescein-5-isothiocyanate