title: A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity.
abstract: A protocol for a fluorescent intercalator displacement (FID) assay useful for establishing DNA binding selectivity, affinity, stoichiometry, and binding site size, and for distinguishing modes of DNA binding is presented.
authors.affiliation: Gilead Sciences, Foster City, California, USA.
authors.forename: Winston C
authors.initials: WC
authors.lastname: Tse
authors.forename: Dale L
authors.initials: DL
authors.lastname: Boger
mesh_terms: D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004996:Ethidium / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D007364:Intercalating Agents / Q000737:chemistry* / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D004247:DNA; D004996:Ethidium