title: Direct attachment of conjugate groups to the 5' terminus of oligodeoxyribonucleotides.
abstract: This unit gives protocols for the attachment of intercalating and photoreactive conjugate groups to oligodeoxyribonucleotides. Protocols are given for acridine- and psoralen-conjugated oligonucleotides, and include attachment of the linker, preparation of the phosphoramidite, coupling to the oligonucleotide, deprotection, purification, and characterization.
authors.affiliation: Centre de Biophysique Moléculaire, CNRS, Orléans, France.
authors.forename: U
authors.initials: U
authors.lastname: Asseline
authors.forename: N T
authors.initials: NT
authors.lastname: Thuong
mesh_terms: D000166:Acridines / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D001671:Biochemistry / Q000379:methods*; D005363:Ficusin / Q000138:chemical synthesis / Q000737:chemistry; D006900:Hydroxylation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013057:Spectrum Analysis
chemical_list: D000166:Acridines; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D005363:Ficusin