Euthanasia.
Euthanasia of laboratory animals must be performed by trained personnel using appropriate techniques, equipment, and reagents in order to effect a death that is humane and satisfies research requirements. Acceptable methods of euthanasia are painless or minimize distress, and are quick and easy to perform, safe for those performing the procedure, and efficient and economic. They are aesthetically acceptable and are done in the absence of other animals. In addition, these methods do not result in gross histological or histochemical changes that would adversely affect research results. This unit offers protocols for euthanasia employing carbon dioxide asphyxiation (see Basic Protocol 1), pentobarbital overdose (see Basic Protocol 2), exsanguination, and cervical dislocation for the mouse, rat, hamster, and rabbit.
Donovan
John
J
Wyeth Research, Collegeville, Pennsylvania.
Brown
Patricia
P
National Institutes of Health, Bethesda, Maryland.
D000759:Adjuvants, Anesthesia; D000758:Anesthesia / Q000379:methods / Q000662:veterinary; D000827:Animal Welfare; D000818:Animals; D000830:Animals, Laboratory; D002245:Carbon Dioxide; D006224:Cricetinae; D037901:Euthanasia, Animal / Q000379:methods*; D051379:Mice; D009115:Muridae; D010424:Pentobarbital; D011817:Rabbits; D051381:Rats
D000759:Adjuvants, Anesthesia; D002245:Carbon Dioxide; D010424:Pentobarbital