title: Topical poloxamer-188 improves blood flow following thermal injury in rat mesenteric microvasculature.
abstract: Microvascular changes of sludging and stasis are indications of thermal injury in tissue. This study investigates whether microvascular thermal injury can be decreased via topical application of poloxamer-188. Rat mesenteric microvessels were thermally injured and topically suffused with either Ringer's solution (control) or 5% poloxamer-188 in Ringer's solution (experiment). Blood flow was characterized in microvessels as normal or abnormal (ie, sludging and stasis). Topical treatment with poloxamer-188 reduced the percentage of capillaries with abnormal blood flow from 62% to 23%. In venules, this treatment resulted in a decrease from 54% to 34%. These results demonstrated that topically applied poloxamer-188 dramatically reduces the microvascular changes of sludging and stasis because of thermal injury in rat mesenteric microvessels.
authors.affiliation: Department of Plastic Surgery, University of Virginia Health System, Charlottesville, VA, USA. sab6k@virginia.edu
authors.forename: Shawn A
authors.initials: SA
authors.lastname: Birchenough
authors.forename: George T
authors.initials: GT
authors.lastname: Rodeheaver
authors.forename: Raymond F
authors.initials: RF
authors.lastname: Morgan
authors.forename: Shayn M
authors.initials: SM
authors.lastname: Peirce
authors.forename: Adam J
authors.initials: AJ
authors.lastname: Katz
mesh_terms: D000287:Administration, Topical; D000818:Animals; D002056:Burns / Q000188:drug therapy*; D004195:Disease Models, Animal; D006439:Hemodynamics; D007552:Isotonic Solutions / Q000494:pharmacology; D008297:Male; D008643:Mesentery / Q000098:blood supply / Q000187:drug effects*; D008833:Microcirculation / Q000187:drug effects*; D020442:Poloxamer / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012039:Regional Blood Flow / Q000187:drug effects*; D000077331:Ringer's Solution; D013501:Surface-Active Agents / Q000494:pharmacology*
chemical_list: D007552:Isotonic Solutions; D013501:Surface-Active Agents; D020442:Poloxamer; D000077331:Ringer's Solution