Thrombophilic mutations in pre-eclampsia and pregnancy-induced hypertension.
OBJECTIVE
The aim of the present study was to determine the existence or prevalence of thrombophilic markers such as Factor V Leiden, prothrombin G20210A, protein S, protein C, activated protein C and anti-thrombin in pre-eclampsia and pregnancy-induced hypertensive patients.


METHODS
Blood samples were collected from a total number of 124 women at the maternity unit, University of Malaya Medical Center. These included 49 patients with pre-eclampsia, 63 patients with pregnancy-induced hypertension and 12 normal pregnant women. DNA was extracted from the blood samples. Factor V Leiden (Taq I) and prothrombin G20210A (Hind III) genotyping was done on polymerase chain reaction-restriction fragment length polymorphism. Anti-thrombin activity and the concentrations of protein C, protein S and activated protein C were measured using the IL Coagulation System (Hemosil).


RESULTS
Of the 124 subjects, one pre-eclampsia patient was homozygous for Factor V Leiden mutation but prothrombin G20210A mutation was not present in any of the subjects. The subject with Factor V Leiden mutation also had a low activated protein C resistance and a low protein S concentration.


CONCLUSIONS
Factor V Leiden mutation is present in the Asian population and may very well serve as one of the genetic factors responsible for pre-eclampsia and other adverse pregnancy outcomes.
Omar
Siti Z
SZ
Department of Obstetrics and Gynecology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Qvist
Rajes
R
Khaing
Si L
SL
Muniandy
Sekaran
S
Bhalla
Sunil
S
D004247:DNA / Q000737:chemistry / Q000235:genetics; D005165:Factor V / Q000235:genetics / Q000378:metabolism; D005260:Female; D005819:Genetic Markers; D006801:Humans; D046110:Hypertension, Pregnancy-Induced / Q000097:blood / Q000235:genetics*; D008296:Malaysia; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics*; D011247:Pregnancy; D011486:Protein C / Q000235:genetics / Q000378:metabolism; D017293:Protein S / Q000235:genetics / Q000378:metabolism; D011516:Prothrombin / Q000235:genetics / Q000378:metabolism; D019851:Thrombophilia / Q000097:blood / Q000235:genetics*
D005819:Genetic Markers; D011486:Protein C; D017293:Protein S; C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin; D004247:DNA