title: Glucose transporter 5 is undetectable in outer hair cells and does not contribute to cochlear amplification.
abstract: Glucose transporter 5 (Glut5) is a high-affinity fructose transporter. It was proposed to be a motor protein or part of the motor complex required for cochlear amplification in outer hair cells (OHCs). Here we show that, in contrast to previous reports, Glut5 is undetectable, and possibly absent, in OHCs harvested from wildtype mice. Further, Glut5-deficient mice display normal OHC morphology and motor function (i.e., nonlinear capacitance and electromotility) and normal cochlear sensitivity and frequency selectivity. We conclude that Glut5 is not required for OHC motility or cochlear amplification.
authors.affiliation: Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wu
authors.forename: Xiang
authors.initials: X
authors.lastname: Wang
authors.forename: Jiangang
authors.initials: J
authors.lastname: Gao
authors.forename: Yiling
authors.initials: Y
authors.lastname: Yu
authors.forename: Shuping
authors.initials: S
authors.lastname: Jia
authors.forename: Jing
authors.initials: J
authors.lastname: Zheng
authors.forename: Peter
authors.initials: P
authors.lastname: Dallos
authors.forename: David Z Z
authors.initials: DZ
authors.lastname: He
authors.forename: MaryAnn
authors.initials: M
authors.lastname: Cheatham
authors.forename: Jian
authors.initials: J
authors.lastname: Zuo
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D002678:Chimera; D002923:Cilia / Q000378:metabolism / Q000648:ultrastructure; D003051:Cochlea / Q000378:metabolism* / Q000648:ultrastructure; D004734:Energy Metabolism / Q000235:genetics; D005632:Fructose / Q000378:metabolism; D051246:Glucose Transport Proteins, Facilitative / Q000235:genetics* / Q000378:metabolism*; D051296:Glucose Transporter Type 5; D018072:Hair Cells, Auditory, Outer / Q000378:metabolism* / Q000648:ultrastructure; D006309:Hearing / Q000502:physiology; D008297:Male; D040542:Mechanotransduction, Cellular / Q000502:physiology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D020409:Molecular Motor Proteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D013234:Stem Cells; D013737:Testis / Q000378:metabolism / Q000648:ultrastructure
chemical_list: D051246:Glucose Transport Proteins, Facilitative; D051296:Glucose Transporter Type 5; D020409:Molecular Motor Proteins; C498720:Pres protein, mouse; D012333:RNA, Messenger; C465351:Slc2a5 protein, mouse; D005632:Fructose