title: Overexpression of AQP5, a putative oncogene, promotes cell growth and transformation.
abstract: Overexpression of several aquaporins has been reported in different types of human cancer but the role of AQPs in human carcinogenesis has not yet been clearly defined. Here, we demonstrate that ectopic expression of human AQP5 (hAQP5), a water channel expressed in lung, salivary glands, and kidney, induces many phenotypic changes characteristic of transformation both in vitro and in vivo. Furthermore, the cell proliferative ability of AQP5 appears to be dependent upon the phosphorylation of a cAMP-protein kinase (PKA) consensus site located in a cytoplasmic loop of AQP5. In addition, phosphorylation of the PKA consensus site was found to be phosphorylated preferentially in tumors. These findings altogether indicate that hAQP5 plays an important role in human carcinogenesis and, furthermore, provide an attractive therapeutic target.
authors.affiliation: Department of Otolaryngology - Head and Neck Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
authors.forename: Janghee
authors.initials: J
authors.lastname: Woo
authors.forename: Juna
authors.initials: J
authors.lastname: Lee
authors.forename: Young Kwang
authors.initials: YK
authors.lastname: Chae
authors.forename: Myoung Sook
authors.initials: MS
authors.lastname: Kim
authors.forename: Jin Hyen
authors.initials: JH
authors.lastname: Baek
authors.forename: Jong Chul
authors.initials: JC
authors.lastname: Park
authors.forename: Min Joo
authors.initials: MJ
authors.lastname: Park
authors.forename: Ian M
authors.initials: IM
authors.lastname: Smith
authors.forename: Barry
authors.initials: B
authors.lastname: Trink
authors.forename: Edward
authors.initials: E
authors.lastname: Ratovitski
authors.forename: Taekyul
authors.initials: T
authors.lastname: Lee
authors.forename: Bumsoo
authors.initials: B
authors.lastname: Park
authors.forename: Se Jin
authors.initials: SJ
authors.lastname: Jang
authors.forename: Jean C
authors.initials: JC
authors.lastname: Soria
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Califano
authors.forename: David
authors.initials: D
authors.lastname: Sidransky
authors.forename: Chulso
authors.initials: C
authors.lastname: Moon
mesh_terms: D000818:Animals; D051402:Aquaporin 5 / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002471:Cell Transformation, Neoplastic / Q000235:genetics; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D009369:Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation; D011518:Proto-Oncogene Proteins / Q000187:drug effects / Q000378:metabolism*; D034741:RNA, Small Interfering / Q000494:pharmacology; D015854:Up-Regulation / Q000235:genetics
chemical_list: C496036:AQP5 protein, human; D051402:Aquaporin 5; D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering