An investigation into structural changes due to deuteration.
Perdeuteration of proteins is becoming more commonplace and the assumption is in general that deuteration does not affect protein structure. In this work, the effect of deuteration on structure is examined by data mining, largely of the Cambridge Structural Database but also of the Inorganic Crystal Structure Database, for deuterated and hydrogenated pairs of small-molecule structures analysed by neutron and X-ray crystallography. Differences between these small-molecule structures have been calculated and the results thus far follow the initial assumption. However, functional changes are known, e.g. D(2)O is toxic to living systems but H(2)O is not, kinetics change, small pH to pD changes occur, proteins stiffen in D(2)O and ferroelectrics alter their properties.
Fisher
S J
SJ
School of Chemistry, Brunswick Street, The University of Manchester, Manchester M13 9PL, UK.
Helliwell
J R
JR
D018360:Crystallography, X-Ray; D030562:Databases, Protein; D003903:Deuterium / Q000737:chemistry*; D017666:Deuterium Oxide / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D033363:Neutron Diffraction; D011506:Proteins / Q000737:chemistry*
D011506:Proteins; D003903:Deuterium; D017666:Deuterium Oxide