title: Separation of cytidine 5'-triphosphate biosynthesized from cytidine 5'-monophosphate on ion-exchange resin and HPLC analysis of cytidine compounds.
abstract: Conditions were studied in the biosynthesis of cytidine 5'-triphosphate (CTP) from cytidine 5'-monophosphate (CMP). A 201 x 7 anion ion-exchange resin was applied for the separation of CTP from CMP. Adsorption isotherm and elution conditions (eluant, eluant concentration, flow rate, sample volume loaded) were investigated. At the same time, a new high-performance liquid chromatography on an anion ion-exchange column WAX-1 with UV detector at 260 nm was developed to measure CMP, cytidine 5'-diphosphate (CDP), and CTP. The retention time for CMP, CDP, and CTP are 0.723, 1.448, and 4.432 min, respectively. This new rapid high-performance liquid chromatography (HPLC) method for the analysis of cytidine compounds in biological sample has a wide linear range with high precision and repeatability.
authors.affiliation: College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
authors.forename: Lu-E
authors.initials: LE
authors.lastname: Shi
authors.forename: Guo-Qing
authors.initials: GQ
authors.lastname: Ying
authors.forename: Zhen-Xing
authors.initials: ZX
authors.lastname: Tang
authors.forename: Yu
authors.initials: Y
authors.lastname: Yi
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Shan
authors.forename: Hua-Zhang
authors.initials: HZ
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D001709:Biotechnology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D003565:Cytidine Diphosphate / Q000032:analysis; D003568:Cytidine Monophosphate / Q000032:analysis / Q000378:metabolism*; D003570:Cytidine Triphosphate / Q000032:analysis / Q000096:biosynthesis* / Q000302:isolation & purification*; D007475:Ion Exchange Resins; D012441:Saccharomyces cerevisiae / Q000378:metabolism
chemical_list: D007475:Ion Exchange Resins; D003565:Cytidine Diphosphate; D003570:Cytidine Triphosphate; D003568:Cytidine Monophosphate