title: Phenolic composition in grape (Vitis vinifera L. cv. Malbec) ripened with different solar UV-B radiation levels by capillary zone electrophoresis.
abstract: The responses of Vitis vinifera L. cv. Malbec to different solar ultraviolet-B radiation (UV-B) levels were assessed in two contrasting situations, under sunlight with full UV-B (+UV-B) and filtered UV-B (-UV-B), in three different locations at 500, 1000, and 1500 m above sea level (asl). To evaluate the effects of radiation, a simple, accurate, and rapid method for the separation and simultaneous determination of representative phenolic compounds in grape berry skins by capillary zone electrophoresis was developed. Separation was carried out in less than 20 min with 20 mM sodium tetraborate buffer containing 30% methanol, pH 9.00. The procedure is fast and reliable, and extracted grape berry skins can be directly analyzed without prior sample cleanup procedure. Berry skins from the +UV-B treatment at 1500 m asl showed the highest levels of total polyphenols anthocyanins, and resveratrol, compared with the -UV-B treatment at this altitude.
authors.affiliation: Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Alte. Brown 500, 5505 Chacras de Coria, Argentina.
authors.forename: Federico
authors.initials: F
authors.lastname: Berli
authors.forename: José
authors.initials: J
authors.lastname: D'Angelo
authors.forename: Bruno
authors.initials: B
authors.lastname: Cavagnaro
authors.forename: Rubén
authors.initials: R
authors.lastname: Bottini
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Wuilloud
authors.forename: M Fernanda
authors.initials: MF
authors.lastname: Silva
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D019075:Electrophoresis, Capillary; D005419:Flavonoids / Q000032:analysis; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development*; D010636:Phenols / Q000032:analysis*; D059808:Polyphenols; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis; D013472:Sunlight; D014466:Ultraviolet Rays; D027843:Vitis / Q000737:chemistry / Q000254:growth & development*
chemical_list: D000872:Anthocyanins; D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D013267:Stilbenes; D000077185:Resveratrol