[Effects of grape seed proanthocyanidins on oxidative stress in primary rat hippocampal neurons].
OBJECTIVE
To investigate the effects of grape seed proanthocyanidins (GSP) on the oxidative stress induced by hydrogen peroxide (H2O2) in primary rat hippocampal neurons.


METHODS
Primary cultured hippocampal neurons injury model by H2O2 was established. Morphological changes of neuron cells were visualized. The viability of hippocampal neurons was detected by MTT. The contents of malonidaldelyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) in the cells were measured by chemochromatometry respectively.


RESULTS
The cell activity decreased remarkably induced by 1 mmol/L H2O2 for 24 hours (P < 0.01), the levels of MDA and ROS were increased, and SOD, CAT, GSH-Px level were decreased in cells. GSP could reduce MDA and ROS level obviously, and increase SOD, CAT and GSH-Px level in cells.


CONCLUSIONS
GSP has protective effect on hippocampal neurons injury induced by H2O2. The protective effect may be related to protecting cell membrane, increasing the activity of clearance enzyme of free radical and inhibiting lipid peroxidation.
Tang
Ying
Y
Department of Medical Experiment, Wuhan General Hospital of Guangzhou Military Region, Wuhan 430070, China.
Diao
Bo
B
Wang
Xiao-kun
XK
Yang
Li
L
Zou
Rui
R
Deng
Hui-ling
HL
D000818:Animals; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology* / Q000187:drug effects; D006861:Hydrogen Peroxide; D008315:Malondialdehyde / Q000378:metabolism; D009474:Neurons / Q000166:cytology / Q000187:drug effects*; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D044945:Proanthocyanidins / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*
D044945:Proanthocyanidins; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase