[Application of two-dimensional liquid chromatography coupled with mass spectrometry for the separation of active compounds in Gegen Qinlian decoction].
An system of two-dimensional liquid chromatography (2D-LC) coupled with mass spectrometry was developed for the separation of components in Gegen Qinlian decoction, a complex traditional Chinese medicine. A CN column was used in the first dimensional chromatography, with gradient elution using water and methanol as the mobile phase A and mobile phase B, respectively; an ODS column was used in the second dimensional chromatography, with gradient elution using 20 mmol/L ammonium acetate buffer and acetonitrile as the mobile phase A and mobile phase B, respectively. The effluent was detected by mass spectrometry with electrospray ionization (ESI)/atmospheric pressure ionization (APCI) combined ion source and positive and negative scan modes. The components in Gegen Qinlian decoction was separated with the 2D-LC system with peak capacity up to 8000. Compared with UV detection, MS detected more components, especially in the negative mode. The 2D-LC/MS system provides higher peak capacity, better resolution and the capacity of peak identification. It is suitable for the analysis of complex samples such as the traditional Chinese medicine.
Guo
Fei
F
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China. vivian_gf@163.com
Wang
Yan
Y
Wang
Renfeng
R
Yan
Chao
C
D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification*; D013058:Mass Spectrometry / Q000379:methods*
D004365:Drugs, Chinese Herbal; C542794:gegenqinlian