[Identification and purification of Tyrophagus putrescentiae allergens].
OBJECTIVE
To identify and purify the allergens of Tyrophagus putrescentiae.


METHODS
Tyrophagus putrescentiae extract was analyzed by SDS-PAGE and Western blotting, and was partially purified by DE52 anion-exchange chromatography and HiLoad 16, 60 Superdex 200 prep grade Size-exclusion chromatography.


RESULTS
23 bands were found after SDS-PAGE with comparative molecular weight (Mr) of 177,000, 118,000, 107,000, 70,000, 67,000, 60,000, 52,000, 45,000, 41,000, 40,000, 38,000, 37,000, 35,000, 27,000, 23,000, 22,000, 18,000, 17,000, 16,000, 15,000, 14,000, 13,000 and 12,000. Five allergens were detected by Western blotting with Mr 128,000, 67,000-70,000, 36,000-37,000, 18,000 and 16,000, respectively. The positive reaction rate of 3 allergens, with Mr 128,000, 67,000-70,000 and 36,000-37,000, were 100%, while that of other 2 allergens with Mr 18,000 and 16,000 was 77.8% and 44.4% respectively. The allergen with Mr 18,000 was purified by anion-exchange chromatography and Size-exclusion chromatography.


CONCLUSIONS
Four major allergens and one minor allergen from Tyrophagus putrescentiae have been identified.
Huang
Zhi-Jian
ZJ
Allergy and Immunology Institute, Shenzhen University, Shenzhen 518060, China.
Liu
Zhi-Gang
ZG
D040041:Acaridae / Q000276:immunology*; D000485:Allergens / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D000818:Animals; D002850:Chromatography, Gel / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D008970:Molecular Weight
D000485:Allergens