title: Detection of protein-protein interactions by coprecipitation.
abstract: Co-precipitation of proteins from whole cell extracts is a valuable approach to test for physical interactions between proteins of interest. It may be the method of choice, or may be used in conjunction with other procedures to detect protein-protein interactions, such as two-hybrid analyis and co-purification schemes, and tests of physical associations using purified proteins.
authors.affiliation: Harvard Medical School, Boston, Massachusetts.
authors.forename: Elaine A
authors.initials: EA
authors.lastname: Elion
mesh_terms: D011232:Chemical Precipitation; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins