Fabrication of 2D ordered films of tobacco mosaic virus (TMV): processing morphology correlations for convective assembly.
Biological colloids, and in particular viruses, have demonstrated substantial potential as scaffolds for nanoparticle arrays. However, the large-area, low-cost, and rapid assembly of viruses, such as by traditional colloidal processing techniques, is not well-established. Systematic exploration of processing space (virus concentration, assembly speed, and substrate surface energy) for the convective assembly method enables the fabrication of films of rod-shaped viruses (tobacco mosaic virus, TMV) with a high degree of long-range order. Monolayer assemblies several centimeters in length are comprised of TMV aligned parallel to the direction of assembly. Increasing TMV concentration and reducing assembly speed resulted in well-ordered viral layering ( N = 2 to N = 12); however, the top virus layer exhibits varying degrees of in-plane disorder.
Wargacki
Stephen P
SP
Air Force Research Laboratory, WPAFB, Ohio, and Universal Technology Corporation, Dayton, Ohio, USA.
Pate
Brian
B
Vaia
Richard A
RA
D002213:Capsid; D003102:Colloids / Q000737:chemistry; D008422:Materials Testing; D018625:Microscopy, Atomic Force; D008961:Models, Structural; D012367:RNA, Viral; D012542:Scattering, Radiation; D012825:Silicon / Q000737:chemistry*; D013499:Surface Properties; D013816:Thermodynamics; D014027:Tobacco Mosaic Virus / Q000378:metabolism*; D014764:Viral Proteins / Q000737:chemistry; D014779:Virus Replication; D014965:X-Rays
D003102:Colloids; D012367:RNA, Viral; D014764:Viral Proteins; D012825:Silicon