title: Insulin acutely upregulates protein expression of the fatty acid transporter CD36 in human skeletal muscle in vivo.
abstract: Enhanced fatty acid uptake may lead to the accumulation of lipid intermediates. This is related to insulin resistance and type 2 diabetes mellitus. Rodent studies suggest that fatty acid transporters are acutely regulated by insulin. We investigated differences in fatty acid transporter content before and at the end of a hyperinsulinemic euglycemic clamp in skeletal muscle (m. vastus lateralis) of obese, glucose-intolerant men (IGT) and obese normal glucose tolerant controls (NGT). The fatty acid transporter FAT/CD36 protein content increased 1.5-fold (P < 0.05) after 3-hrs of insulin stimulation with no difference between IGT and control subjects. No change was seen in cytosolic fatty acid binding protein (FABPc) protein content. The increase in FAT/CD36 protein content was positively related to insulin resistance as measured during the clamp (r = 0.56, P < 0.05). An increase in FAT/CD36 protein content in skeletal muscle may result in a higher fractional extraction of fatty acids (larger relative uptake) after a meal, enhancing triglyceride accumulation in the muscle. We conclude that also in obese humans the FAT/CD36 protein content in skeletal muscle is dynamically regulated by insulin in vivo on the short term.
authors.affiliation: From the Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands. E.Corpeleijn@hb.unimaas.nl
authors.forename: E
authors.initials: E
authors.lastname: Corpeleijn
authors.forename: M M A L
authors.initials: MM
authors.lastname: Pelsers
authors.forename: S
authors.initials: S
authors.lastname: Soenen
authors.forename: M
authors.initials: M
authors.lastname: Mensink
authors.forename: F G
authors.initials: FG
authors.lastname: Bouwman
authors.forename: M E
authors.initials: ME
authors.lastname: Kooi
authors.forename: W H M
authors.initials: WH
authors.lastname: Saris
authors.forename: J F C
authors.initials: JF
authors.lastname: Glatz
authors.forename: E E
authors.initials: EE
authors.lastname: Blaak
mesh_terms: D018955:CD36 Antigens / Q000187:drug effects* / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D006801:Humans; D007328:Insulin / Q000097:blood / Q000502:physiology*; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000378:metabolism; D009765:Obesity / Q000378:metabolism*; D014280:Triglycerides / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D018955:CD36 Antigens; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D007328:Insulin; D014280:Triglycerides