Enhanced bioaffinity sensing using surface plasmons, surface enzyme reactions, nanoparticles and diffraction gratings.
This paper introduces a novel approach to surface bioaffinity sensing based on the adsorption of nanoparticles onto a gold diffraction grating that supports the excitation of planar surface plasmons. A surface enzymatic amplification reaction is also incorporated into the detection scheme to enhance the sensitivity and utility of the nanoparticle-enhanced diffraction grating (NEDG) sensors. As a demonstration, the detection of microRNA is described where a combination of a surface polymerase reaction and DNA-modified nanoparticles is used to detect the bioaffinity adsorption of the target onto the probe-functionalized gold grating surface. The enzymatically-amplified NEDG sensors possess a great potential for a wide range of applications including the detection of biosecurity agents, DNA and RNA viruses, biomarkers, and proteins.
Lee
Hye Jin
HJ
Department of Chemistry, Kyungpook National University, Daegu 702-701, Korea. hyejinlee@knu.ac.kr
Wark
Alastair W
AW
Corn
Robert M
RM
D000327:Adsorption; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D006046:Gold; D006801:Humans; D053768:Metal Nanoparticles; D035683:MicroRNAs / Q000032:analysis; D044085:Microfluidics; D036103:Nanotechnology; D016133:Polymerase Chain Reaction / Q000379:methods; D020349:Surface Plasmon Resonance
D035683:MicroRNAs; D006046:Gold