Calcium flux assay in Xenopus oocytes.
Many G protein-coupled receptors of interest to neuroscientists induce transient increases in [Ca(2+)](i), which can be used as a convenient measure of receptor activation in a variety of applications. This unit describes a simple calcium flux assay applied to Xenopus oocytes.
Murphy
P M
PM
National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland, USA.
D000818:Animals; D001692:Biological Transport; D002132:Calcium Radioisotopes / Q000378:metabolism; D020013:Calcium Signaling / Q000502:physiology*; D005260:Female; D009865:Oocytes / Q000378:metabolism*; D014982:Xenopus laevis
D002132:Calcium Radioisotopes