title: Enzymatic synthesis of M(1)G-deoxyribose.
abstract: Adducts formed between electrophiles and nucleic acid bases are believed to play a key role in chemically induced mutations and cancer. M(1)G-dR is an endogenous exocyclic DNA adduct formed by the reaction of the dicarbonyl compound malondialdehyde with a dG residue in DNA. It is an intermediate in the synthesis of a class of modified oligodeoxyribonucleotides that are used to study the mutagenicity and repair of M(1)G. This unit presents methods for synthesizing M(1)G-dR by enzymatic coupling.
authors.affiliation: Vanderbilt University, Nashville, Tennessee.
authors.forename: N C
authors.initials: NC
authors.lastname: Schnetz-Boutaud
authors.forename: M C
authors.initials: MC
authors.lastname: Chapeau
authors.forename: L J
authors.initials: LJ
authors.lastname: Marnett
mesh_terms: D002384:Catalysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007778:Lactobacillus / Q000201:enzymology; D010430:Pentosyltransferases / Q000378:metabolism*; D013939:Thymidine Phosphorylase / Q000378:metabolism
chemical_list: C089188:3-(2'-deoxyribofuranosyl)pyrimido(1,2-a)purin-10(3H)-one; D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D013939:Thymidine Phosphorylase; C021888:nucleoside deoxyribosyltransferase; D003849:Deoxyguanosine