title: Spontaneous amplification of the actinorhodin gene cluster in Streptomyces coelicolor involving native insertion sequence IS466.
abstract: We observed a spontaneous amplification of the Streptomyces coelicolor chromosome, including genes encoding biosynthetic enzymes of the antibiotic actinorhodin. A new junction of two tandem segments has, inserted within it, a third copy of a transposable element existing in two places elsewhere in the chromosome, suggesting its involvement in the amplification mechanism.
authors.affiliation: Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA. ewidenbrant@stanfordalumni.org
authors.forename: E M
authors.initials: EM
authors.lastname: Widenbrant
authors.forename: Hsiu-Hui
authors.initials: HH
authors.lastname: Tsai
authors.forename: Carton W
authors.initials: CW
authors.lastname: Chen
authors.forename: C M
authors.initials: CM
authors.lastname: Kao
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D008957:Models, Genetic; D005810:Multigene Family / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C013390:actinorhodin; D000095622:Benzoisochromanequinones