Detection of phosphorylation by enzymatic techniques.
This unit presents protocols for examining the functional effects elicited by nonspecific acid or alkaline phosphatases that dephosphorylate many phosphoproteins in vitro. Additional protocols describe digestion of phosphoproteins with a protein serine/threonine phosphatase and protein tyrosine phosphatase. A support protocol has been included to identify the radiolabel as (32)P(I) based on its ability to form a complex with ammonium molybdate.
Shenolikar
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Duke University Medical Center, Durham, North Carolina, USA.
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001671:Biochemistry / Q000379:methods*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation
D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D000469:Alkaline Phosphatase; D010749:Phosphoprotein Phosphatases; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases