Construction of immunoglobulin fusion proteins.
Recombinant DNA technology has allowed the preparation of chimeric genes encoding proteins with novel properties. This unit describes the construction and subsequent testing of genes encoding immunoglobulin chimeras. The first protocol details fusion of a protein (or protein fragment) of interest onto an immunoglobulin constant region using a modified version of the expression vector pCDM8. The resulting fusion protein generally retains the functional properties of both the protein of interest and the immunoglobulin constant region; this can be demonstrated as described here.
Hollenbaugh
Diane
D
Bristol-Myers Squibb Pharmaceutical Research Institute, Seattle, Washington, USA.
Aruffo
Alejandro
A
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000235:genetics; D007127:Immunoglobulin Constant Regions / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D007127:Immunoglobulin Constant Regions; D011993:Recombinant Fusion Proteins