title: Oxidative stress and cystic fibrosis-related diabetes: a pilot study in children.
abstract: BACKGROUND
  Cystic fibrosis (CF) is characterized by chronic inflammation with increased oxidative stress. We evaluated the relationship between glucose tolerance and oxidative stress in CF children.
  
  
  METHODS
  Patients 10-18 years old underwent oral glucose tolerance testing (n=31). At 2-h, we assessed blood glutathione and 4-hydroxynonenal-protein adducts (HNE-P), and urine 1,4-dihydroxynonane-mercapturic acid conjugate (DHN-MA). Plasma fatty acid (FA) profile was performed. Patients with impaired glucose tolerance (IGT) were retested 6 to 24 months later and received additional nutritional recommendations (NR) when possible.
  
  
  RESULTS
  Fifty-two percent of patients had normal glucose tolerance (NGT), 42% IGT and 6% cystic fibrosis-related diabetes (CFRD). HNE-P concentrations significantly increased with diabetes (109%). Two-h BG correlated positively with HNE-P and negatively with DHN-MA. FA profile was modified with IGT. Of retested IGT patients, 25% received no NR; they remained IGT at 6 months and progressed to CFRD. Of those who received NR, 67% normalized, 11% remained intolerant and 22% developed CFRD. HNE-P levels dropped (88%) in IGT patients reverting to NGT, increased (94%) in the IGT patients with NR developing CFRD, decreased (90%) with persistent IGT.
  
  
  CONCLUSIONS
  CF children showed evidence of increased oxidative stress with worsening of glucose metabolism. NR may delay the appearance of CFRD.
authors.affiliation: Department of Nutrition, University of Montreal, Montreal, Canada.
authors.forename: Thierry
authors.initials: T
authors.lastname: Ntimbane
authors.forename: Preetha
authors.initials: P
authors.lastname: Krishnamoorthy
authors.forename: Céline
authors.initials: C
authors.lastname: Huot
authors.forename: Laurent
authors.initials: L
authors.lastname: Legault
authors.forename: Sheila V
authors.initials: SV
authors.lastname: Jacob
authors.forename: Suzanne
authors.initials: S
authors.lastname: Brunet
authors.forename: Emile
authors.initials: E
authors.lastname: Levy
authors.forename: Françoise
authors.initials: F
authors.lastname: Guéraud
authors.forename: Larry C
authors.initials: LC
authors.lastname: Lands
authors.forename: Blandine
authors.initials: B
authors.lastname: Comte
mesh_terms: D000111:Acetylcysteine / Q000031:analogs & derivatives / Q000652:urine; D000293:Adolescent; D000704:Analysis of Variance; D002648:Child; D003550:Cystic Fibrosis / Q000150:complications* / Q000503:physiopathology; D003920:Diabetes Mellitus / Q000209:etiology*; D005227:Fatty Acids / Q000097:blood; D005260:Female; D018149:Glucose Intolerance / Q000175:diagnosis* / Q000209:etiology*; D005951:Glucose Tolerance Test; D005978:Glutathione / Q000097:blood; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000097:blood; D018384:Oxidative Stress; D010865:Pilot Projects; D012129:Respiratory Function Tests
chemical_list: C501116:1,4-dihydroxynonene mercapturic acid; D005227:Fatty Acids; C431137:KIRREL1 protein, human; D008565:Membrane Proteins; D005978:Glutathione; D000111:Acetylcysteine