Association and in silico assignment of sequences from turkey BACs.
Bacterial artificial chromosomes (BACs) provide an important resource in genetic mapping. An initial set of BACs corresponding to microsatellite markers in the turkey (Meleagris gallopavo) was isolated from the CHORI-260 turkey BAC library. The selected markers were distributed on both macro- and microchromosomes and included a genetically unlinked marker. End sequences were obtained for a subset of the recovered BACs and compared to the chicken whole genome sequence. Close association of the turkey BAC-end sequences and original marker sequences was generally conserved in the chicken genome. Gene content of the turkey BACs is predicted from the comparative sequence alignments.
Reed
Kent M
KM
Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA. reedx054@umn.edu
Faile
Gretchen M
GM
Kreuth
Stacy B
SB
Chaves
Lee D
LD
Sullivan
Laura M
LM
D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002874:Chromosome Mapping / Q000379:methods / Q000662:veterinary*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D014422:Turkeys / Q000235:genetics*
D004247:DNA