title: Repeated application of an electric field increases BDNF in the brain, enhances spatial learning, and induces infarct tolerance.
abstract: Development of a safe method to increase brain-derived neurotrophic factor (BDNF) in the brain is expected to have utility in enhancing learning and memory, in protecting the brain, and in suppressing appetite. We investigated the effects of whole-body exposure to high voltage electric potential (HELP), which generates an electric field and current density in the body, on BDNF levels in the brain, spatial learning, or resistance to cerebral infarction development after focal ischemia. Adult mice (C57BL/6J) were exposed to 3.5 kV, or 5.8 kV for 5 h a day, making indirect contact with the ground via room air, over 1, 3, 6 or 12 consecutive weeks. After treatment, BDNF levels, performances in the Morris water maze task (MWM), or development of infarct lesion after focal ischemia was analyzed. Treatment with 3.5 kV for 1, 3, 6 or 12 weeks, or with 5.8 kV for 1, 3 or 12 weeks increased BDNF levels in the cortex (P<0.05, one-way ANOVA). Every HELP treatment differentially improved escape latency in the MWM, compared with the corresponding untreated controls (P<0.05, one-way ANOVA). Treatment with 3.5 kV for 6 or 12 weeks, but not with 5.8 kV protected the brain suppressing cerebral infarction development (P<0.05). The HELP treatment with 3.5 kV for 6 or 12 weeks improves spatial learning, gently suppressing body weight gain, and protects the brain against cerebral infarction.
authors.affiliation: Laboratory for Cerebrovascular Disorders, Research Institute of National Cardio-Vascular Center, Suita, Osaka 565-8565, Japan. hyanamot@res.ncvc.go.jp
authors.forename: Hiroji
authors.initials: H
authors.lastname: Yanamoto
authors.forename: Susumu
authors.initials: S
authors.lastname: Miyamoto
authors.forename: Yukako
authors.initials: Y
authors.lastname: Nakajo
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Nakano
authors.forename: Takuya
authors.initials: T
authors.lastname: Hori
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Naritomi
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Kikuchi
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001794:Blood Pressure / Q000528:radiation effects; D001921:Brain / Q000502:physiology* / Q000528:radiation effects*; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D002544:Cerebral Infarction / Q000209:etiology / Q000517:prevention & control*; D002560:Cerebrovascular Circulation / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004574:Electromagnetic Fields / Q000009:adverse effects*; D006339:Heart Rate / Q000528:radiation effects; D007511:Ischemia / Q000150:complications; D007858:Learning / Q000528:radiation effects*; D008297:Male; D018782:Maze Learning / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D013037:Spatial Behavior / Q000528:radiation effects*; D013550:Swimming; D013997:Time Factors
chemical_list: D019208:Brain-Derived Neurotrophic Factor