Genotoxic effects of white fluorescent light on human lymphocytes in vitro.
Sources of light beams such as white fluorescent light, are present in our daily life to meet the needs of life in the modern world. This study was conducted with the objective of determining the possible genotoxic, cytotoxic and aneugenic effects caused by this agent in different stages of the cell cycle (G0/early G1, S, and late G2), using different cytogenetic parameters (sister chromatid exchanges--SCE, chromosome aberrations--CA, and detection of aneugenic effects) in lymphocytes from temporary cultures of human peripheral blood. WFL showed a genotoxic effect in vitro, expressed by an increase in the frequency of SCE's, regardless of the cell cycle stage. However, no increase in the frequency of CAs was observed. In addition, disturbances in cell cycle kinetics and chromosomal segregation were also observed. Taken together, such data may contribute to a better understanding and a different management in the use of phototherapy for some pathological conditions.
Amorim
Marúcia Irena Medeiros
MI
Human Cytogenetics Laboratory, Institute of Biological Sciences, Federal University of Pará, Belém, PA, Brazil.
Ferrari
Iris
I
Bahia
Marcelo de Oliveira
Mde O
Lima
Patrícia Danielle Lima
PD
Cardoso
Plínio Cerqueira Dos Santos
PC
Khayat
André Salim
AS
Cabral
Isabel Rosa
IR
Burbano
Rommel Rodríguez
RR
D002453:Cell Cycle / Q000528:radiation effects; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D006801:Humans; D008027:Light / Q000009:adverse effects*; D008214:Lymphocytes / Q000528:radiation effects* / Q000648:ultrastructure; D008940:Mitotic Index; D011840:Radiation, Nonionizing / Q000009:adverse effects*; D012854:Sister Chromatid Exchange / Q000528:radiation effects*