title: Oligonucleotide arrays for expression monitoring.
abstract: This unit provides protocols for the amplification and labeling of mRNA (and the necessary controls) for hybridization to oligonucleotide arrays. It also describes methods for processing and normalizing the raw gene expression data in preparation for clustering and further analysis.
authors.affiliation: Genetics Institute, Cambridge, Massachusetts, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Byrne
authors.forename: M Z
authors.initials: MZ
authors.lastname: Whitley
authors.forename: M T
authors.initials: MT
authors.lastname: Follettie
mesh_terms: D018076:DNA, Complementary / Q000235:genetics / Q000302:isolation & purification; D020869:Gene Expression Profiling / Q000379:methods*; D005826:Genetics, Medical; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D018076:DNA, Complementary; D012333:RNA, Messenger