title: Theory and application of fluorescence microscopy.
abstract: The growing importance in biology and especially in neurobiology of fluorescence microscopy is due to (1) the extraordinary development of new fluorescent molecular probes and (2) the development of improved low light level imaging systems and confocal microscopy techniques. This overview covers fluorescent molecular probes, filters and filter sets, multiband filters and multidye fluorescence, light sources, microscope objectives, image resolution and the point-spread function, and general steps for immunolabeling.
authors.affiliation: University of California Berkeley, Berkeley, California, USA.
authors.forename: D
authors.initials: D
authors.lastname: Coling
authors.forename: B
authors.initials: B
authors.lastname: Kachar
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D015335:Molecular Probes; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D015335:Molecular Probes