title: Isolation and functional use of human NK T cells.
abstract: Isolation and Functional Use of Human NK T Cells (Mark A. Exley andSteven P. Balk, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts; S. Brian Wilson, Massachusetts General Hospital, Harvard Medical School, Cambridge, Massachusetts). This unit describes methods for the isolation and functional analysis of human invariant NK T cells. The first protocol uses standard multicolor flow cytometry methods and staining reagents specific for V to generate invariant NK T cell clones. Support protocols for secondary stimulations and for rapid expansion of invariant NK T cell clones and functional analysis of these cells are also included.
authors.affiliation: Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: Mark A
authors.initials: MA
authors.lastname: Exley
authors.forename: Steven P
authors.initials: SP
authors.lastname: Balk
authors.forename: S Brian
authors.initials: SB
authors.lastname: Wilson
mesh_terms: D002469:Cell Separation / Q000379:methods; D002999:Clone Cells; D006801:Humans; D007158:Immunologic Techniques; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology / Q000378:metabolism