[Detection of the expression level of S100C gene in lung cancer tissue by RT-PCR and construction and identification of prokaryotic expression vector].
OBJECTIVE
To analyze the expression level of S100C gene in lung cancer tissue and to construct prokaryotic expression vector pET30a-S100C in order to select fusion antibody in further study.


METHODS
mRNA expression level of S100C gene in lung cancer tissue and ambient tissue in 26 cases was detected by RT-PCR with p-actin as references. The full-length cDNA of S100C was cloned by RT-PCR. The amplified DNA fragments were ligated into pET30a vector and then transformed into E. coli stain JM109. The positive clones were screened out and indentified.


RESULTS
The expression level of S100C in lung cancer tissue was lower than that in ambient tissue. The difference between the two groups was statistically significant (P < 0.05). The prokaryotic expression victor of S100C was successfully constructed. The sequence homogenuity was up to 100%.


CONCLUSIONS
The down-regulation expression of S100C gene probably takes important part in the carcinogenisis of lung cancer.
Zhao
Xiangfeng
X
Department of Occupational Health and Occupational Medicine, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
Zhang
Huizhen
H
Jin
Lei
L
Yang
Jiyao
J
D000328:Adult; D000368:Aged; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D005822:Genetic Vectors; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D009418:S100 Proteins / Q000235:genetics / Q000378:metabolism*
D018076:DNA, Complementary; D012333:RNA, Messenger; D011994:Recombinant Proteins; D009418:S100 Proteins; C072337:S100A11 protein, human