title: Chromosomal instability in rhesus macaque preimplantation embryos.
abstract: OBJECTIVE
  To establish a relevant animal model to systematically investigate chromosomal instability in human oocytes and preimplantation embryos.
  
  
  METHODS
  Prospective rhesus monkey IVF study.
  
  
  METHODS
  Academic laboratory, Oregon National Primate Research Center and Caribbean Primate Research Center.
  
  
  METHODS
  Young rhesus macaque females.
  
  
  METHODS
  In vitro produced entire rhesus macaque preimplantation embryos were cytogenetically assessed using a five-color fluorescent in situ hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X, and Y, using human bacterial artificial chromosome probes.
  
  
  METHODS
  Chromosomal abnormality rates in preimplantation embryos from young rhesus macaque females were established.
  
  
  RESULTS
  Fifty preimplantation embryos, displaying good morphology and normal development, were analyzed from 11 young rhesus macaque females. Overall, 27 embryos (54%) were normal, 11 embryos (22%) mosaic, 3 embryos (6%) chaotic, 2 embryos (4%) aneuploid, 3 embryos (6%) haploid, and 4 embryos (8%) triploid.
  
  
  CONCLUSIONS
  These data indicate that in vitro produced rhesus macaque and human preimplantation embryos exhibit similar numerical chromosomal aberrations. Rhesus macaques appear to be a suitable animal model for investigating the origin of chromosomal instability observed in human preimplantation embryos.
authors.affiliation: Departments of Obstetrics & Gynecology and Physiology, CS Mott Center for Human Growth and Development, Wayne State University, School of Medicine, Detroit, Michigan 48201, USA. cdupont@med.wayne.edu
authors.forename: Cathérine
authors.initials: C
authors.lastname: Dupont
authors.forename: Lutz
authors.initials: L
authors.lastname: Froenicke
authors.forename: Leslie A
authors.initials: LA
authors.lastname: Lyons
authors.forename: Barry D
authors.initials: BD
authors.lastname: Bavister
authors.forename: Carol A
authors.initials: CA
authors.lastname: Brenner
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism*; D002478:Cells, Cultured; D043171:Chromosomal Instability / Q000502:physiology*; D053938:DNA Fragmentation; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000235:genetics; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D017404:In Situ Hybridization, Fluorescence; D008253:Macaca mulatta / Q000235:genetics* / Q000502:physiology*; D008297:Male; D023421:Models, Animal; D009030:Mosaicism; D011003:Ploidies; D011247:Pregnancy