title: Association and in silico assignment of sequences from turkey BACs.
abstract: Bacterial artificial chromosomes (BACs) provide an important resource in genetic mapping. An initial set of BACs corresponding to microsatellite markers in the turkey (Meleagris gallopavo) was isolated from the CHORI-260 turkey BAC library. The selected markers were distributed on both macro- and microchromosomes and included a genetically unlinked marker. End sequences were obtained for a subset of the recovered BACs and compared to the chicken whole genome sequence. Close association of the turkey BAC-end sequences and original marker sequences was generally conserved in the chicken genome. Gene content of the turkey BACs is predicted from the comparative sequence alignments.
authors.affiliation: Department of Veterinary and Biomedical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, Minnesota 55108, USA. reedx054@umn.edu
authors.forename: Kent M
authors.initials: KM
authors.lastname: Reed
authors.forename: Gretchen M
authors.initials: GM
authors.lastname: Faile
authors.forename: Stacy B
authors.initials: SB
authors.lastname: Kreuth
authors.forename: Lee D
authors.initials: LD
authors.lastname: Chaves
authors.forename: Laura M
authors.initials: LM
authors.lastname: Sullivan
mesh_terms: D000818:Animals; D001483:Base Sequence; D002645:Chickens; D002874:Chromosome Mapping / Q000379:methods / Q000662:veterinary*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D004247:DNA / Q000235:genetics; D008040:Genetic Linkage; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D014422:Turkeys / Q000235:genetics*
chemical_list: D004247:DNA