title: Enhanced propagation of fish nodaviruses in BF-2 cells persistently infected with snakehead retrovirus (SnRV).
abstract: Fish nodaviruses are causative agents of viral nervous necrosis causing high mortality in cultured marine fishes around the world. The first successful isolation of fish nodavirus was made with SSN-1 cells, which are persistently infected with snakehead retrovirus (SnRV). In the present study, a BF-2 cell line persistently infected with SnRV (PI-BF-2) was established to evaluate the influence of SnRV on the production of fish nodavirus. The PI-BF-2 cells were slightly more slender than BF-2 cells, but no difference was observed in propagation rate between both cell lines. No difference was observed in production of SnRV between PI-BF-2 and SSN-1 cell lines. Although both PI-BF-2 and BF-2 cell lines showed no cytopathic effect (CPE) after inoculation of striped jack nervous necrosis virus (SJNNV) and redspotted grouper nervous necrosis virus (RGNNV), these fish nodaviruses could be amplified in BF-2 cells, and moreover, production of fish nodaviruses in the PI-BF-2 cell line was more than 40 times higher than in BF-2 cells. Thus, it was concluded that BF-2 cell permissiveness to fish nodaviruses was enhanced by persistent infection with SnRV. Furthermore, homologous cDNA to genomic RNA of SJNNV was detected from both PI-BF-2 and SSN-1 cell lines persistently infected with SnRV. The amount of nodavirus cDNA in SJNNV-inoculated PI-BF-2 cells was clearly lower than that in SJNNV-inoculated SSN-1 cells.
authors.affiliation: Faculty of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan. jjnishi@fish.hokudai.ac.jp
authors.forename: Toyohiko
authors.initials: T
authors.lastname: Nishizawa
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kokawa
authors.forename: Takako
authors.initials: T
authors.lastname: Wakayama
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kinoshita
authors.forename: Mamoru
authors.initials: M
authors.lastname: Yoshimizu
mesh_terms: D000818:Animals; D002460:Cell Line; D030283:Epsilonretrovirus / Q000235:genetics / Q000254:growth & development / Q000502:physiology*; D015751:Genes, pol / Q000235:genetics; D030381:Nodaviridae / Q000235:genetics / Q000254:growth & development*; D010473:Perciformes / Q000821:virology*; D013997:Time Factors