title: Differential gene expression profiling in HELLP syndrome placentas.
abstract: The aim of this study was to identify differentially expressed genes by suppression subtractive hybridization (SSH) in HELLP placentas. Two cDNA libraries were constructed; HSI (HELLP subtracted induced or upregulated) and HSS (HELLP subtracted suppressed or downregulated). Two hundred eighty-eight cDNA clones were sequenced; 37 were matched to GenBank entries and included genes in cell communication and organization, cellular processes, genetic information processing, and metabolic processes. A subgroup of 11 genes of interest was further selected for real-time quantitative polymerase chain reaction confirmation. Results showed no differences in expression of chosen upregulated genes between HELLP and non-HELLP placentas; 6 HELLP downregulated genes were significantly suppressed. Two genes related to production of secreted proteins, CTHRC1 and SERPINE2. SERPINE2 (PAI-1) is a soluble protease inhibitor and is a potential biomarker by Western blot analysis, and the protein is significantly decreased in HELLP placentas. SERPINE2 might be tested clinically in patients for early diagnosis of HELLP syndrome.
authors.affiliation: Department of Obstetrics and Gynecology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
authors.forename: Bum-Yong
authors.initials: BY
authors.lastname: Kang
authors.forename: Stephen
authors.initials: S
authors.lastname: Tsoi
authors.forename: Shan
authors.initials: S
authors.lastname: Zhu
authors.forename: Shenghui
authors.initials: S
authors.lastname: Su
authors.forename: Helen H
authors.initials: HH
authors.lastname: Kay
mesh_terms: D000328:Adult; D016564:Amyloid beta-Protein Precursor / Q000032:analysis / Q000235:genetics; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015723:Gene Library; D017359:HELLP Syndrome / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010920:Placenta / Q000737:chemistry*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D058255:Protease Nexins; D012333:RNA, Messenger / Q000032:analysis*; D011956:Receptors, Cell Surface / Q000032:analysis / Q000235:genetics; D015203:Reproducibility of Results; D058258:Serpin E2
chemical_list: D016564:Amyloid beta-Protein Precursor; D015415:Biomarkers; C502426:CTHRC1 protein, human; D016326:Extracellular Matrix Proteins; D058255:Protease Nexins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C545486:SERPINE2 protein, human; D058258:Serpin E2