title: A case of pulmonary cryptococcosis in a free-living toad (Bufo bufo).
abstract: Pulmonary cryptococcosis was observed in a free-living adult female common toad (Bufo bufo) that was killed by a vehicle. Both lungs had various eosinophilic, monomorphic, and spherical to elliptical organisms identified as Cryptoccocus spp. The yeasts were demonstrated by Grocott's silver method and the periodic acid-Schiff reaction and the capsule was positive for mucin with a mucicarmine stain. The agent was confirmed by immunohistochemistry, using the monoclonal antibody anti-Cryptococcus neoformans, and by a polymerase chain reaction-based method using a C. neoformans-specific primer. This report, to the best of our knowledge, represents the first case of cryptococcosis in a common toad.
authors.affiliation: Laboratório de Histologia e Anatomia Patológica, Clínicas Veterinárias, Universidade de Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Seixas
authors.forename: Maria da Luz
authors.initials: Mda L
authors.lastname: Martins
authors.forename: Maria
authors.initials: M
authors.lastname: de Lurdes Pinto
authors.forename: Paulo Jorge
authors.initials: PJ
authors.lastname: Travassos
authors.forename: Manuela
authors.initials: M
authors.lastname: Miranda
authors.forename: Maria Dos Anjos
authors.initials: Mdos A
authors.lastname: Pires
mesh_terms: D000818:Animals; D002023:Bufo bufo / Q000382:microbiology*; D003453:Cryptococcosis / Q000175:diagnosis / Q000662:veterinary*; D003455:Cryptococcus neoformans / Q000302:isolation & purification*; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008172:Lung Diseases, Fungal / Q000175:diagnosis / Q000662:veterinary*; D010503:Periodic Acid-Schiff Reaction / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013194:Staining and Labeling / Q000662:veterinary