[Application of fingerprint of active part on extraction techniques for Vernonia anthelmintica].
OBJECTIVE
To probe into feasibility of extraction techniques for Vernonia anthelmintica using HPLC fingerprint of active part as model.


METHODS
The extraction techniques were studied by multi-index test experiment formula evaluation and orthogonal design, which took change of peak area in HPLC fingerprint of active part for Vernonia anthelmintica as indices.


RESULTS
The optimum extracting procedure was as follows: using 10 times volume of water for each time, soaking one hour and then boiling for three times sustaining one hour each.


CONCLUSIONS
It is scientific, simple and applicable.
Yu
Lu-hai
LH
The Clinical Medical Institute of Xinjiang, Urumqi 830001, China.
Li
Yan-ju
YJ
Sun
Li
L
Shang
Jing
J
Xu
Jian-guo
JG
D000465:Algorithms; D000704:Analysis of Variance; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005638:Fruit / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D035741:Vernonia / Q000737:chemistry*; D014867:Water / Q000737:chemistry
D004365:Drugs, Chinese Herbal; D014867:Water