Quantified assessment of terminal density and innervation.
Stereological methods allow for the determination of cell numbers, terminal densities, and, subsequently, the estimation of terminal arbor size within a given brain nucleus. This unit provides an explanation for determining the terminal arbor size of dopaminergic neurons of the nigrostriatal pathway in rodents. In contrast to previously used single-axon reconstructions, these stereological methods allow for quick and easy determination of terminal arbor size.
Finkelstein
David I
DI
Howard Florey Institute, University of Melbourne, Parkville, Victoria, Australia.
Stanic
Davor
D
Parish
Clare L
CL
Drago
John
J
Horne
Malcolm K
MK
D000818:Animals; D035843:Biomedical Research / Q000379:methods*; D003342:Corpus Striatum / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D003712:Dendrites / Q000648:ultrastructure; D004298:Dopamine / Q000378:metabolism*; D051379:Mice; D008968:Molecular Conformation; D009411:Nerve Endings / Q000648:ultrastructure*; D009445:Neuroanatomy / Q000379:methods*; D009474:Neurons / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D051381:Rats; D013378:Substantia Nigra / Q000378:metabolism / Q000502:physiology / Q000648:ultrastructure*; D009435:Synaptic Transmission
D004298:Dopamine