title: Long-term consequences of soman poisoning in mice Part 1. Neuropathology and neuronal regeneration in the amygdala.
abstract: To date, studies on soman-induced neuropathology mainly focused on the hippocampus, since this brain region is a well-delimited area with easily detectable pyramidal neurons. Moreover, the hippocampus is severely damaged after soman exposure leading to a substantial alteration of behavioral mnemonic processes. The neuropathology described in the hippocampus, however, and its behavioral consequences cannot be extrapolated to all other limbic damaged brain areas such as the amygdala. Accordingly, in this inaugural paper, using hemalun-phloxin staining and NeuN immunohistochemistry, the number of damaged and residual healthy neurons was quantified in the amygdala in mice over a 90-day period after soman injection (1.2LD(50) of soman). On post-soman day 1, a moderate neuronal cell death (about 23% of the whole neurons) was evidenced. In parallel, a large quantity of degenerating neurons (about 36% of the whole neurons) occurred in this brain region and survived from post-soman day 1 to day 15. The death of these damaged neurons was initiated on post-soman day 30, and ended on post-soman day 90. Concomitantly, as quantified by NeuN immunohistochemistry, a clear neuronal regeneration was demonstrated in the amygdala of soman-poisoned mice between 60 and 90 days after neurotoxicant exposure. In the companion paper (see part 2), the possible effects of both long-term neuropathology and delayed neuronal regeneration were evaluated on amygdala-driven emotional processes.
authors.affiliation: Département de Toxicologie, CRSSA 24, avenue des Maquis du Grésivaudan, B.P. 87, 38702 LA TRONCHE cedex, France. jmcollombet@crssa.net
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Collombet
authors.forename: Christophe
authors.initials: C
authors.lastname: Piérard
authors.forename: Daniel
authors.initials: D
authors.lastname: Béracochéa
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Coubard
authors.forename: Marie-France
authors.initials: MF
authors.lastname: Burckhart
authors.forename: Elise
authors.initials: E
authors.lastname: Four
authors.forename: Catherine
authors.initials: C
authors.lastname: Masqueliez
authors.forename: Dominique
authors.initials: D
authors.lastname: Baubichon
authors.forename: Guy
authors.initials: G
authors.lastname: Lallement
mesh_terms: D000679:Amygdala / Q000473:pathology*; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002800:Cholinesterase Inhibitors / Q000506:poisoning*; D008297:Male; D051379:Mice; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000473:pathology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D012999:Soman / Q000506:poisoning*; D013997:Time Factors
chemical_list: D002800:Cholinesterase Inhibitors; D012999:Soman; D010751:Phosphopyruvate Hydratase