title: Effects of Cu(2+) and Pb(2+) on different fish species: liver cytochrome P450-dependent monooxygenase activities and FTIR spectra.
abstract: The effects of Cu(2+)-sulfate and Pb(2+)-acetate on carp (Cyprinus carpio L.), silver carp (Hypopthalmichtys molitrix V.) and wels (Silurus glanis L.) were studied. The liver microsomal Cyt P450 content, the EROD, ECOD and APND monooxygenase activities were measured. In vivo treatment with 1 mg L(-1) Cu(2+) significantly elevated the activities of these enzymes and Cyt P450 content in silver carp livers. The high-dose Cu(2+) treatment (10 mg L(-1)) on silver carp caused two-fold higher induction in the P450 dependent monooxygenase isoensymes than in wels. Although the 2 mg kg(-1) treatment with Pb(2+) in carp elevated significantly the P450 content, the EROD isoenzyme activities were significantly decreased after 1 day, showing the destructive effect of metal ion on the enzyme system. In vitro, Cu(2+) and Pb(2+) decreased the Cyt P450 content in the carp liver microsomes and the absorption peak shifted to higher wavelength. Fourier Transform Infrared (FTIR) spectroscopy was used to detect the damaging effects of the heavy metals. According to the inhibitory potency to Cu(2+), the most sensitive isoenzyme was the EROD in wels, the least was the silver carp's isoenzyme. The investigated fish P450 isoenzymes showed, that the Cu(2+) was a stronger inhibitor than Pb(2+).
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Szeged, H-6701 Szeged, Hungary.
authors.forename: Mária
authors.initials: M
authors.lastname: Henczová
authors.forename: Aranka Kiss
authors.initials: AK
authors.lastname: Deér
authors.forename: Adrienn
authors.initials: A
authors.lastname: Filla
authors.forename: Viktória
authors.initials: V
authors.lastname: Komlósi
authors.forename: János
authors.initials: J
authors.lastname: Mink
mesh_terms: D015660:7-Alkoxycoumarin O-Dealkylase / Q000037:antagonists & inhibitors / Q000378:metabolism; D000818:Animals; D002347:Carps / Q000378:metabolism*; D002397:Catfishes / Q000378:metabolism*; D019327:Copper Sulfate / Q000633:toxicity*; D019363:Cytochrome P-450 CYP1A1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D065607:Cytochrome P-450 Enzyme Inhibitors; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000633:toxicity*; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D008862:Microsomes, Liver / Q000187:drug effects / Q000378:metabolism; D009942:Organometallic Compounds / Q000633:toxicity*; D017550:Spectroscopy, Fourier Transform Infrared; D013379:Substrate Specificity; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D065607:Cytochrome P-450 Enzyme Inhibitors; D004791:Enzyme Inhibitors; D009942:Organometallic Compounds; D014874:Water Pollutants, Chemical; D003577:Cytochrome P-450 Enzyme System; D015660:7-Alkoxycoumarin O-Dealkylase; D019363:Cytochrome P-450 CYP1A1; D019327:Copper Sulfate; C008261:lead acetate