Fluorescent in situ hybridization as a screening test for HER2 amplification in G2 and G3 breast cancers of lobular and ductal histotype and metastases.
The aim of the present study was to evaluate the effectiveness of fluorescence in situ hybridisation (FISH), as a screening test, in moderately- (G2) or poorly- (G3) differentiated breast cancers of the ductal (IDC) and lobular (ILC) histotypes and distant metastases. HER2 FISH was performed on 486 G2 and 477 G3 both of IDC and ILC histotypes and in 241 metastases. A significant difference in the HER2 amplification was observed between G2 (14.8%) and G3 (31.9%), with no difference according to the histotype. However, the rate of amplification increased to 36% in the G2/hormone receptor-negative cases as compared to 10.6% in the G2/receptor-positive cases (p<0.0001). HER2 was amplified in 17% of metastases with some differences depending on the location. These data suggest that the HER2 FISH analysis may be an effective screening test in breast cancer metastases and G3 tumors, irrespective of the hormone receptor status or presence of lymphovascular invasion.
Castellano
Isabella
I
Department of Biomedical Sciences and Human Oncology, University of Turin, 10126 Turin, Italy.
Sapino
Anna
A
Arisio
Riccardo
R
Viale
Giuseppe
G
Bussolati
Gianni
G
Bandelloni
Roberto
R
Barresi
Gaetano
G
Bersiga
Alessandra
A
Bordi
Cesare
C
Botti
Gerardo
G
Cosimi
Fabia
F
D'Amore
Emanuele
E
Doglioni
Claudio
C
Marchetti
Antonio
A
Nappi
Oscar
O
Romeo
Francesco
F
Roncalli
Massimo
M
Russo
Rosa
R
Santinelli
Alfredo
A
Spagnoli
Luigi Giusto
LG
Tanda
Francesco
F
Tricomi
Paolo
P
Trentini
Gianpaolo
G
Zanconati
Fabrizio
F
Iurlaro
Monica
M
D005260:Female; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008403:Mass Screening / Q000379:methods; D008495:Medical Oncology / Q000379:methods; D015233:Models, Statistical; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging / Q000379:methods; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D015203:Reproducibility of Results; D013997:Time Factors
D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human