title: p14ARF is capable of promoting HIV-1 tat degradation.
abstract: The p14(ARF) tumor suppressor functions as 'oncogenic checkpoint' that prevents unrestricted cellular proliferation in response to oncogenic signaling. Albeit, the major pathway through which ARF operates is the ARF-Mdm2-p53 axis, ARF directly binds to and inactivates transcription function of a number of DNA-bound activators. In the present study we show that p14(ARF) inhibits transcription activation of HIV-1 LTR promoter activity by Tat protein. Tat protein is a RNA-bound transcriptional activator whose function is strictly required for HIV-1 replication. We determined that p14(ARF) inhibits Tat transactivation of HIV-1 LTR by promoting Tat degradation via an ubiquitin-independent pathway.
authors.affiliation: Department of Structural and Functional Biology, University of Naples Federico II, Naples, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Gargano
authors.forename: Marianna
authors.initials: M
authors.lastname: Fiorillo
authors.forename: Stefano
authors.initials: S
authors.lastname: Amente
authors.forename: Barbara
authors.initials: B
authors.lastname: Majello
authors.forename: Luigi
authors.initials: L
authors.lastname: Lania
mesh_terms: D002850:Chromatography, Gel; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D015696:Gene Products, tat / Q000378:metabolism*; D016325:HIV Long Terminal Repeat / Q000235:genetics* / Q000502:physiology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation; D025803:Tumor Suppressor Protein p14ARF / Q000378:metabolism*
chemical_list: D015696:Gene Products, tat; D025803:Tumor Suppressor Protein p14ARF