title: Quality control assessment of human immunodeficiency virus type 2 (HIV-2) viral load quantification assays: results from an international collaboration on HIV-2 infection in 2006.
abstract: Human immunodeficiency virus type 2 (HIV-2) RNA quantification assays used in nine laboratories of the ACHI(E)V(2E) (A Collaboration on HIV-2 Infection) study group were evaluated. In a blinded experimental design, laboratories quantified three series of aliquots of an HIV-2 subtype A strain, each at a different theoretical viral load. Quantification varied between laboratories, and international standardization of quantification assays is strongly needed.
authors.affiliation: APHP, Hôpital Bichat-Claude Bernard, Laboratoire de Virologie, Paris, 75018 France. florence.damond@bch.aphp.fr
authors.forename: Florence
authors.initials: F
authors.lastname: Damond
authors.forename: Antoine
authors.initials: A
authors.lastname: Benard
authors.forename: Jean
authors.initials: J
authors.lastname: Ruelle
authors.forename: Abraham
authors.initials: A
authors.lastname: Alabi
authors.forename: Bernd
authors.initials: B
authors.lastname: Kupfer
authors.forename: Perpetua
authors.initials: P
authors.lastname: Gomes
authors.forename: Berta
authors.initials: B
authors.lastname: Rodes
authors.forename: Jan
authors.initials: J
authors.lastname: Albert
authors.forename: Jürg
authors.initials: J
authors.lastname: Böni
authors.forename: Jeremy
authors.initials: J
authors.lastname: Garson
authors.forename: Bridget
authors.initials: B
authors.lastname: Ferns
authors.forename: Sophie
authors.initials: S
authors.lastname: Matheron
authors.forename: Geneviève
authors.initials: G
authors.lastname: Chene
authors.forename: Françoise
authors.initials: F
authors.lastname: Brun-Vezinet
mesh_terms: D003299:Cooperative Behavior; D015658:HIV Infections / Q000821:virology*; D015498:HIV-2 / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D038622:Internationality; D007753:Laboratories / Q000592:standards; D011786:Quality Control; D012367:RNA, Viral / Q000097:blood / Q000592:standards*; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results; D019562:Viral Load / Q000592:standards*
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic