Purification of immunoglobulin G.
IgG can be purified, as described here, by ammonium sulfate precipitation followed by size-exclusion (SE) chromatography. This is the least expensive option available for purification of antibodies. Protein A- and protein G-affinity chromatography are the fastest methods for purifying antibodies, but they are not effective for all subclasses of rat antibody. A protocol for affinity chromatography using anti-rat antibody is provided for purification of rat antibodies. Ion-exchange (IEX) chromatography is described for purifying intact monoclonal and polyclonal antibodies and antibody fragments.
Andrew
S M
SM
Lancaster University, Lancaster, United Kingdom.
Titus
J A
JA
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification*; D009419:Nerve Tissue Proteins / Q000737:chemistry / Q000276:immunology; D051381:Rats; D013205:Staphylococcal Protein A / Q000737:chemistry / Q000276:immunology
D000911:Antibodies, Monoclonal; C029152:G-substrate; D007074:Immunoglobulin G; D009419:Nerve Tissue Proteins; D013205:Staphylococcal Protein A