Single-strand conformation polymorphism analysis using capillary electrophoresis.
Single-strand conformation polymorphism (SSCP) is one of the most frequently used mutation detection methods. This unit describes a method of SSCP with automated analysis by capillary electrophoresis in order to increase the capacity and throughput. A protocol is provided for sample preparation. For a medium throughput laboratory, a single capillary instrument, as described in this unit, may be quite sufficient. In many cases, however, screening for mutations in large population groups requires a high throughput, and this is best obtained through the use of a multi-capillary instrument, as discussed.
Larsen
Lars Allan
LA
Statens Serum Institut, Copenhagen, Denmark.
Christiansen
Michael
M
Vuust
Jens
J
Andersen
Paal Skytt
PS
D001483:Base Sequence; D017931:DNA Primers; D019075:Electrophoresis, Capillary / Q000379:methods*; D009154:Mutation; D018807:Polymorphism, Single-Stranded Conformational
D017931:DNA Primers