title: 5'-Iodination of solid-phase-linked oligodeoxyribonucleotides.
abstract: 5'-Iodinated oligodeoxyribonucleotides readily react with 3'-phosphorothioated DNA in the presence of a complementary template to yield a conjugate that is identical to natural DNA in every respect except that one oxygen atom in the phosphodiester backbone is replaced by a sulfur atom. The 5'-iodo group is easily converted to a variety of other functional groups and will quickly react with thiol-containing labels to yield stable thioether conjugates. This unit presents manual and automated procedures for converting the 5'-hydroxyl of protected CPG-bound oligodeoxyribonucleotides to an iodo group and for releasing and purifying the products.
authors.affiliation: Stanford University, Stanford, California, USA.
authors.forename: Eric T
authors.initials: ET
authors.lastname: Kool
authors.forename: Gregory P
authors.initials: GP
authors.lastname: Miller
mesh_terms: D004247:DNA / Q000737:chemistry; D054879:Halogenation; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry
chemical_list: D009838:Oligodeoxyribonucleotides; D010710:Phosphates; C083120:phosphorodithioic acid; D004247:DNA