title: Probing RNA structure with chemical reagents and enzymes.
abstract: This unit provides thorough coverage of the most useful chemical and enzyme probes that can be used to examine RNA secondary and tertiary structure. Footprinting methods are presented using dimethyl sulfate, diethyl pyrocarbonate, ethylnitrosourea, kethoxal, CMCT, and nucleases. For chemical probes, both strand scission and primer extension detection protocols are included.
authors.affiliation: University of Michigan, Ann Arbor, Michigan, USA.
authors.forename: W A
authors.initials: WA
authors.lastname: Ziehler
authors.forename: D R
authors.initials: DR
authors.lastname: Engelke
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D017122:Bacteriophage T4 / Q000201:enzymology; D020029:Base Pairing; D001671:Biochemistry / Q000379:methods*; D002074:Butanones; D003028:CME-Carbodiimide / Q000031:analogs & derivatives / Q000737:chemistry; D004047:Diethyl Pyrocarbonate / Q000737:chemistry; D004586:Electrophoresis; D004720:Endonucleases / Q000378:metabolism*; D005038:Ethylnitrosourea / Q000737:chemistry; D007202:Indicators and Reagents; D009690:Nucleic Acid Conformation / Q000187:drug effects*; D010761:Phosphorus Radioisotopes; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000378:metabolism*; D012015:Reference Standards; D013463:Sulfuric Acid Esters / Q000737:chemistry
chemical_list: D000447:Aldehydes; D002074:Butanones; D007202:Indicators and Reagents; D010761:Phosphorus Radioisotopes; C034477:RNA primers; D013463:Sulfuric Acid Esters; C060331:1-cyclohexyl-3-(2-(4-morpholinyl)ethyl)carbodiimide; D003028:CME-Carbodiimide; D012313:RNA; C005135:kethoxal; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D004720:Endonucleases; C007482:dimethyl sulfate; D004047:Diethyl Pyrocarbonate; D005038:Ethylnitrosourea