Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection.
Adenoviruses (AdV) are commonly involved in acute respiratory infections (ARI), which cause high morbidity and mortality in children. AdV are grouped in six species (A-F), which are associated with a wide range of diseases. The aim of this study was to identify the AdV species infecting non-hospitalized Mexican children with ARI symptoms, attending to the same school. For that, a PCR/RFLP assay was designed for a region of the hexon gene, which was chosen, based on the bioinformatical analysis of AdV genomes obtained from GenBank. A total of 100 children's nasopharyngeal samples were collected from January to June, 2005, and used for viral isolation in A549 cells and PCR/RFLP analysis. Only 15 samples produced cytopathic effect, and in all of them AdV C was identified. AdV C was also identified in eight additional nasopharyngeal samples which were negative for viral isolation. In summary, this outpatient population showed a rate of AdV infection of 23%, and only AdV C was detected.
Rosete
Dora P
DP
Laboratorio de Virología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Naciona, México, D.F., México.
Manjarrez
María Eugenia
ME
Barrón
Blanca L
BL
D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002648:Child; D004262:DNA Restriction Enzymes / Q000032:analysis; D005260:Female; D005819:Genetic Markers; D016679:Genome, Viral; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D009305:Nasopharynx / Q000821:virology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D012621:Seasons
D005819:Genetic Markers; D004262:DNA Restriction Enzymes