Dentin phosphophoryn promotes cellular migration of human dental pulp cells.
Dentin phosphophoryn (DPP) is a dentin sialophosphoprotein gene product that has an RGD motif and repeat sequences of aspartic acid and phosphoserine. To date, the function of DPP in the early stage of reparative dentin formation still remains unclear. The objective of this study was to evaluate the effects of DPP on pulp cell migration and proliferation. DPP promoted cell migration in a concentration-dependent manner, thus increasing it by about 3-fold at 1000 ng/mL compared with the control, but it had no effect on cell proliferation. Dephosphorylated DPP also promoted cell migration, similarly to DPP. However, cell migration was significantly suppressed by the addition of alphavbeta3 integrin antibody to the medium. Furthermore, porcine DPP-derived RGD peptide, but not its mutant RAD peptide, significantly promoted cell migration. These results indicated that the RGD motif of DPP plays an important role in the migration of human dental pulp cells.
Yasuda
Yoshiyuki
Y
Division of Clinical Cariology and Endodontology, Department of Oral Rehabilitation, School of Dentistry, Health Sciences University of Hokkaido, Hokkaido, Japan. yasuda@hokuiryo-u.ac.jp
Izumikawa
Masanobu
M
Okamoto
Kuniaki
K
Tsukuba
Takayuki
T
Saito
Takashi
T
D000328:Adult; D000409:Alanine; D020816:Amino Acid Motifs; D000818:Animals; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D017124:Conserved Sequence; D003782:Dental Pulp / Q000166:cytology*; D003809:Dentin, Secondary / Q000737:chemistry; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000502:physiology; D008969:Molecular Sequence Data; D020125:Mutation, Missense; D009842:Oligopeptides; D010750:Phosphoproteins / Q000737:chemistry / Q000502:physiology*; D012795:Sialoglycoproteins / Q000737:chemistry / Q000502:physiology*; D013552:Swine
D039302:Integrin alphaVbeta3; D009842:Oligopeptides; D010750:Phosphoproteins; D012795:Sialoglycoproteins; C017630:phosphophoryn; C047981:arginyl-glycyl-aspartic acid; D000409:Alanine