title: Dialysis and concentration of protein solutions.
abstract: Conventional dialysis separates small molecules from large molecules by allowing diffusion of only the small molecules through selectively permeable membranes. This appendix describes dialysis of large- and small-volume samples using cellulose membranes with pore sizes designed to exclude molecules above a selected molecular weight. A Support Protocol describes preparation of membranes for dialysis and discusses issues related to the selection of membranes including commercial kits.
authors.affiliation: Lancaster University, Lancaster, United Kingdom.
authors.forename: S M
authors.initials: SM
authors.lastname: Andrew
authors.forename: J A
authors.initials: JA
authors.lastname: Titus
authors.forename: L
authors.initials: L
authors.lastname: Zumstein
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D003956:Dialysis / Q000379:methods*; D015314:Dialysis Solutions; D008567:Membranes, Artificial; D011506:Proteins / Q000302:isolation & purification*
chemical_list: D015314:Dialysis Solutions; D008567:Membranes, Artificial; D011506:Proteins; D002482:Cellulose