title: Erect wing regulates synaptic growth in Drosophila by integration of multiple signaling pathways.
abstract: BACKGROUND
  Formation of synaptic connections is a dynamic and highly regulated process. Little is known about the gene networks that regulate synaptic growth and how they balance stimulatory and restrictive signals.
  
  
  RESULTS
  Here we show that the neuronally expressed transcription factor gene erect wing (ewg) is a major target of the RNA binding protein ELAV and that EWG restricts synaptic growth at neuromuscular junctions. Using a functional genomics approach we demonstrate that EWG acts primarily through increasing mRNA levels of genes involved in transcriptional and post-transcriptional regulation of gene expression, while genes at the end of the regulatory expression hierarchy (effector genes) represent only a minor portion, indicating an extensive regulatory network. Among EWG-regulated genes are components of Wingless and Notch signaling pathways. In a clonal analysis we demonstrate that EWG genetically interacts with Wingless and Notch, and also with TGF-beta and AP-1 pathways in the regulation of synaptic growth.
  
  
  CONCLUSIONS
  Our results show that EWG restricts synaptic growth by integrating multiple cellular signaling pathways into an extensive regulatory gene expression network.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK.
authors.forename: Irmgard U
authors.initials: IU
authors.lastname: Haussmann
authors.forename: Kalpana
authors.initials: K
authors.lastname: White
authors.forename: Matthias
authors.initials: M
authors.lastname: Soller
mesh_terms: D000818:Animals; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics / Q000502:physiology*; D051959:ELAV Proteins; D005786:Gene Expression Regulation; D053263:Gene Regulatory Networks; D009474:Neurons; D009479:Neuropeptides / Q000502:physiology*; D051880:Receptors, Notch / Q000235:genetics; D015398:Signal Transduction; D013569:Synapses / Q000648:ultrastructure*; D018808:Transcription Factor AP-1 / Q000235:genetics; D014157:Transcription Factors / Q000502:physiology*; D016212:Transforming Growth Factor beta / Q000235:genetics; D051155:Wnt1 Protein / Q000235:genetics
chemical_list: D029721:Drosophila Proteins; D051959:ELAV Proteins; C081334:EWG protein, Drosophila; D009479:Neuropeptides; D051880:Receptors, Notch; D018808:Transcription Factor AP-1; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D051155:Wnt1 Protein; C074622:wg protein, Drosophila