title: Studies on the mechanism of the peroxyoxalate chemiluminescence reaction: part 2. Further identification of intermediates using 2D EXSY 13C nuclear magnetic resonance spectroscopy.
abstract: Further consideration has been given to the reaction pathway of a model peroxyoxalate chemiluminescence system. Again utilising doubly labelled oxalyl chloride and anhydrous hydrogen peroxide, 2D EXSY (13)C nuclear magnetic resonance (NMR) spectroscopy experiments allowed for the characterisation of unknown products and key intermediate species on the dark side of the peroxyoxalate chemiluminescence reaction. Exchange spectroscopy afforded elucidation of a scheme comprised of two distinct mechanistic pathways, one of which contributes to chemiluminescence. (13)C NMR experiments carried out at varied reagent molar ratios demonstrated that excess amounts of hydrogen peroxide favoured formation of 1,2-dioxetanedione: the intermediate that, upon thermolysis, has been long thought to interact with a fluorophore to produce light.
authors.affiliation: Alphapharm, 15 Garnet Street, Carole Park, Queensland 4300, Australia.
authors.forename: Sarah A
authors.initials: SA
authors.lastname: Tonkin
authors.forename: Richard
authors.initials: R
authors.lastname: Bos
authors.forename: Gail A
authors.initials: GA
authors.lastname: Dyson
authors.forename: Kieran F
authors.initials: KF
authors.lastname: Lim
authors.forename: Richard A
authors.initials: RA
authors.lastname: Russell
authors.forename: Simon P
authors.initials: SP
authors.lastname: Watson
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Hindson
authors.forename: Neil W
authors.initials: NW
authors.lastname: Barnett
mesh_terms: D002247:Carbon Isotopes; D003198:Computer Simulation; D049449:Luminescence; D009682:Magnetic Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D010070:Oxalates / Q000138:chemical synthesis / Q000737:chemistry*; D011789:Quantum Theory; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002247:Carbon Isotopes; D010070:Oxalates; C044622:peroxyoxalate