Modification of the 5' terminus of oligonucleotides for attachment of reporter and conjugate groups.
Reporter and conjugate groups can be added directly to the 5' terminus of oligonucleotides by appropriate modification. Conjugate groups can be used to increase the affinity of complementary strands, induce irreversible modification of target sequences, or enable sequences to recognize and permeate target cell membranes. This overview discusses the 5' modifications that can be used and strategies for the covalent attachment of ligands to the modified oligonucleotides. Step-by-step protocols for attachment of conjugate groups are given elsewhere in the series.
Thuong
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Centre de Biophysique Moléculaire, CNRS, Orléans, France.
Asseline
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D000818:Animals; D001671:Biochemistry / Q000379:methods*; D004952:Esters / Q000737:chemistry; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry; D017905:Phosphites / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
D004952:Esters; D009841:Oligonucleotides; D010710:Phosphates; D017905:Phosphites; D013438:Sulfhydryl Compounds