Dissecting the molecular basis of the regulation of wood formation by auxin in hybrid aspen.
Indole acetic acid (auxin) is a key regulator of wood formation, and an observed overlap between auxin concentration gradient and developing secondary xylem cells has led to the hypothesis that auxin regulates wood formation by acting as a morphogen. We dissected the role of auxin in wood formation by identifying the auxin-responsive transcriptome in wood-forming tissues and investigating alterations in wood formation in transgenic hybrid aspen plants (Populus tremula x Populus tremuloides) with perturbed auxin signaling. We showed that auxin-responsive genes in wood-forming tissues respond dynamically to changes in cellular auxin levels. However, the expression patterns of most of the auxin-responsive genes displayed limited correlation with the auxin concentration across this developmental zone. Perturbing auxin signaling by reducing auxin responsiveness reduced the cambial cell division activity, caused spatial deregulation of cell division of the cambial initials, and led to reductions in not only radial but also axial dimensions of fibers and vessels. We propose that, instead of acting as a morphogen, changes in auxin concentration in developing secondary xylem cells may provide important regulatory cues that modulate the expression of a few key regulators; these, in turn, may control the global gene expression patterns that are essential for normal secondary xylem development.
Nilsson
Jeanette
J
Umeå Plant Science Centre, Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90183 Umeå, Sweden.
Karlberg
Anna
A
Antti
Henrik
H
Lopez-Vernaza
Manuel
M
Mellerowicz
Ewa
E
Perrot-Rechenmann
Catherine
C
Sandberg
Göran
G
Bhalerao
Rishikesh P
RP
D000595:Amino Acid Sequence; D002455:Cell Division; D003001:Cloning, Molecular; D007210:Indoleacetic Acids / Q000378:metabolism*; D008969:Molecular Sequence Data; D016296:Mutagenesis; D009154:Mutation; D030821:Plants, Genetically Modified; D012333:RNA, Messenger / Q000235:genetics; D015398:Signal Transduction; D014197:Trees / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D014934:Wood; D052584:Xylem
D007210:Indoleacetic Acids; D012333:RNA, Messenger