title: Affinity-based biosensors for biomolecular interaction analysis.
abstract: This overview presents an introduction to affinity-based biosensors, most notably the BIACORE. These biosensors detect molecular interactions by immobilizing one of the interactants (the ligand or target molecule) on the surface of a sensor chip. A solution containing the other interactant is then applied to the chip. Binding of analyte to the surface results in a response proportional to the mass bound. A discussion of sensor chips and practical applications is also provided.
authors.affiliation: BIACORE AB, Uppsala, Sweden.
authors.forename: K
authors.initials: K
authors.lastname: Gunnarsson
mesh_terms: D000937:Antigen-Antibody Reactions; D001665:Binding Sites; D015374:Biosensing Techniques; D011485:Protein Binding; D011487:Protein Conformation; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods; D013816:Thermodynamics
chemical_list: D011506:Proteins