title: Nectin-like molecule 1 is a glycoprotein with a single N-glycosylation site at N290KS which influences its adhesion activity.
abstract: Nectin-like molecule 1 (NECL1)/CADM3/IGSF4B/TSLL1/SynCAM3, from now on referred to as NECL1, is a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule which has Ca(2+)-independent homo- or heterophilic cell-cell adhesion activity and plays an important role in the formation of synapses, axon bundles and myelinated axons. Here we first detected the expression of NECL1 in human fetal and adult brains, and mouse brains at different developmental stages. The results indicated that two bands with molecular weights of about 62 kDa and 48 kDa were found in human fetal brain, while only one band with a molecular weight of about 48 kDa was found in human adult brain; two bands with molecular weights of about 62 kDa and 48 kDa whose expression level gradually increased were also found from mouse E16 to P14, while only one band with a molecular weight of about 48 kDa was found from P14. Bioinformatics analysis showed there were two putative N-glycosylation sites within human NECL1 at positions N25LS and N290KS and within mouse Necl1 at positions N23LS and N288KS, respectively. There was no O-glycosylation site in either human NECL1 or mouse Necl1. Based on the results of N-Glycosidase F treatment with human fetal brain tissue and lysates from transient transfection with human wild-type or glycosylation site mutant NECL1 in 293ET cells, we demonstrated that human NECL1 is an N-linked glycoprotein with a single glycosylation site at position N290KS. Cell aggregation assay further showed there was an increased adhesion activity after the glycosylation site mutation of NECL1 molecule.
authors.affiliation: National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, National Human Genome Center, Beijing, China. gaojing_pumc@yahoo.com.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.forename: Tao
authors.initials: T
authors.lastname: Chen
authors.forename: Guangyu
authors.initials: G
authors.lastname: Hu
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Gong
authors.forename: Boqin
authors.initials: B
authors.lastname: Qiang
authors.forename: Jiangang
authors.initials: J
authors.lastname: Yuan
authors.forename: Xiaozhong
authors.initials: X
authors.lastname: Peng
mesh_terms: D000328:Adult; D019943:Amino Acid Substitution; D000818:Animals; D001369:Axons / Q000378:metabolism*; D001921:Brain / Q000196:embryology / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules; D002460:Cell Line; D006031:Glycosylation; D006801:Humans; D007136:Immunoglobulins / Q000235:genetics / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D020125:Mutation, Missense; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D013569:Synapses / Q000378:metabolism*
chemical_list: C498599:CADM3 protein, human; D015815:Cell Adhesion Molecules; C498600:Igsf4b protein, mouse; D007136:Immunoglobulins; D008562:Membrane Glycoproteins; D008565:Membrane Proteins; D009419:Nerve Tissue Proteins