title: A new combined "interrupted-continuous" microvascular anastomotic technique. Experimental study and clinical application.
abstract: A new combined interrupted-continuous microvascular anastomotic technique is introduced, using four interrupted stay stitches and continuous suturing in between. Its efficacy is compared with the simple interrupted and continuous techniques. Microanastomoses were performed in the femoral artery and vein of rats. The patency, anastomosis time, and blood loss from the suture line of all anastomoses were recorded. Also, the sites of all anastomoses were harvested and histologically examined for lumen stenosis, media regeneration, and endothelial lining. Experimentation findings showed 100% patency rate in all groups. The combined and the continuous techniques were significantly faster and watertight compared with the simple interrupted. The only difference in pathology findings was the development of significant lumen stenosis in vein anastomoses performed with the continuous technique, compared with the other two techniques. The combined technique was clinically used in 16 free flap transfers and ensured 100% patency in all arterial and vein anastomoses, as well as survival of all flaps. In conclusion, the combined technique was found to be fast and easy to perform and ensured the same patency rate as the simple interrupted and continuous techniques. It also diminished the lumen stenosis effect of the continuous technique in vein anastomoses. Therefore, we suggest its use in both experimental studies and clinical cases.
authors.affiliation: Department of Plastic Surgery and Burns, University of Ioannina School of Medicine, Ioannina, Greece.
authors.forename: Efstathios G
authors.initials: EG
authors.lastname: Lykoudis
authors.forename: Georgia-Alexandra Ch
authors.initials: GA
authors.lastname: Spyropoulou
authors.forename: George N
authors.initials: GN
authors.lastname: Liadakis
authors.forename: Eugenia T
authors.initials: ET
authors.lastname: Papaliodi
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D005263:Femoral Artery / Q000601:surgery; D006801:Humans; D008866:Microsurgery; D051381:Rats; D017207:Rats, Sprague-Dawley; D013536:Suture Techniques; D014654:Vascular Patency