Honokiol, a natural therapeutic candidate, induces apoptosis and inhibits angiogenesis of ovarian tumor cells.
OBJECTIVE
To observe the anti-tumor activities of honokiol on human ovarian tumor in vitro and in vivo.


METHODS
Cells were treated with honokiol, and the effects on proliferation and apoptosis were examined by MTT, DNA ladder, Hoechst staining, and flow cytometry assays. Expression of Bcl-2 members and caspase-3 were assessed. Measurements of tumor volume and microvessel densities (MVDs) were performed.


RESULTS
Honokiol significantly inhibited proliferation and induced apoptosis, with alteration of Bcl-2 members and caspase-3. Administration of honokiol to tumor-bearing animals decreased MVD and resulted in inhibition of tumor growth.


CONCLUSIONS
Honokiol could induce apoptosis and inhibit angiogenesis in vitro and in vivo, suggesting a novel and attractive therapeutic candidate for ovarian tumor treatment.
Li
Zhengyu
Z
Department of Obstetrics and Gynecology, West China Second Hospital of Sichuan University, Renmin South Road, Chengdu 610041, People's Republic of China.
Liu
Yi
Y
Zhao
Xia
X
Pan
Xiaoling
X
Yin
Rutie
R
Huang
Canhua
C
Chen
Lijuan
L
Wei
Yuquan
Y
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001713:Biphenyl Compounds / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D051379:Mice; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000188:drug therapy*; D010051:Ovarian Neoplasms / Q000188:drug therapy*; D000498:Allyl Compounds; D010636:Phenols
D020533:Angiogenesis Inhibitors; D001713:Biphenyl Compounds; D017705:Lignans; C005499:honokiol; D000498:Allyl Compounds; D010636:Phenols