title: Zymography of metalloproteinases.
abstract: Zymography is an electrophoretic technique enabling visualization of the number and approximate size of peptidases in a sample on the basis of their hydrolysis of a protein substrate within the gel. The technique is particularly useful for analyzing the peptidase composition of complex biological samples because visualization depends directly on proteolytic activity. This unit presents a representative zymography protocol for the study of matrix metallopeptidases (MMPs).
authors.affiliation: Imperial College London, London, United Kingdom.
authors.forename: Linda
authors.initials: L
authors.lastname: Troeberg
authors.affiliation: Imperial College London, London, United Kingdom.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nagase
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006868:Hydrolysis; D020782:Matrix Metalloproteinases / Q000737:chemistry*; D008970:Molecular Weight; D013379:Substrate Specificity
chemical_list: D020782:Matrix Metalloproteinases