title: Evidence for subdomain flexibility in Drosophila melanogaster acetylcholinesterase.
abstract: The catalytic domain of the acetylcholinesterases is composed of a single polypeptide chain, the folding of which determines two subdomains. We have linked these two subdomains by mutating two residues, I327 and D375, to cysteines, to form a disulfide bridge. As a consequence, the hydrodynamic radius of the protein was reduced, suggesting that there is some flexibility in the subdomain connection. In addition to the smaller size, the mutated protein is more stable than the wild-type protein. Therefore, the flexibility between the two domains is a weak point in terms of protein stability. As expected from the location of the disulfide bond at the rim of the active site, the kinetic studies show that it affects interactions with peripheral ligands and the entrance of some of the bulkier substrates, like o-nitrophenyl acetate. In addition, the mutations affect the catalytic step for o-nitrophenyl acetate and phosphorylation by organophosphates, suggesting that this movement between the two subdomains is connected with the cooperativity between the peripheral and catalytic sites.
authors.affiliation: Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, 1000 Ljubljana, Slovenia. stojan@mf.uni-lj.si
authors.forename: Jure
authors.initials: J
authors.lastname: Stojan
authors.forename: Caroline
authors.initials: C
authors.lastname: Ladurantie
authors.forename: Omid Ranei
authors.initials: OR
authors.lastname: Siadat
authors.forename: Laurent
authors.initials: L
authors.lastname: Paquereau
authors.forename: Didier
authors.initials: D
authors.lastname: Fournier
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D002850:Chromatography, Gel; D004220:Disulfides / Q000737:chemistry / Q000378:metabolism; D004331:Drosophila melanogaster / Q000201:enzymology* / Q000235:genetics; D006868:Hydrolysis; D007700:Kinetics; D008958:Models, Molecular; D009154:Mutation / Q000235:genetics; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity
chemical_list: D004220:Disulfides; D000110:Acetylcholinesterase