Liquid chromatography-tandem mass spectrometry assay for the quantitation of plagiochin E and its main metabolite plagiochin E glucuronides in rat plasma.
Plagiochin E, a macrocyclic bisbibenzyl isolated from liverwort Marchantia polymorpha, was found to have antifungal activity. To evaluate the pharmacokinetics of plagiochin E in rats, a sensitive and specific liquid chromatography/tandem mass spectrometric (LC-MS/MS) method was developed and validated for the quantitation of plagiochin E and its total conjugated metabolites in rat plasma. For detection, a Sciex API 4000 LC-MS/MS with a TurboIonSpray ionization (ESI) inlet in the negative ion-multiple reaction monitoring (MRM) mode was used. The plasma samples were pretreated by a simple liquid-liquid extraction with ethyl acetate. The concentration of plagiochin E parent form was determined directly and the concentration of plagiochin E conjugated metabolites was assayed in the form of plagiochin E after treatment with beta-glucuronidase/sulfatase. The statistical evaluation for this method reveals excellent linearity, accuracy and precision for the range of concentrations 0.5-1000.0 ng/mL. The method had a lower limit of quantification (LLOQ) of 0.5 ng/mL for plagiochin E in 50 microL of plasma. The method was successfully applied to the characterization of the pharmacokinetic profile of plagiochin E in rats after an oral and an intravenous administration.
Xing
Jie
J
School of Pharmaceutical Sciences, Shandong University, 44 West Wenhua Road, Jinan 250012, PR China.
Lv
Beibei
B
Xie
Chunfeng
C
Qu
Jianbo
J
Lou
Hongxiang
H
D000097:Acetonitriles / Q000737:chemistry; D000818:Animals; D001681:Biological Assay; D001952:Bridged-Ring Compounds / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002021:Buffers; D002138:Calibration; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D004304:Dosage Forms; D004355:Drug Stability; D005615:Freezing; D005966:Glucuronidase / Q000494:pharmacology; D020719:Glucuronides / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D006863:Hydrogen-Ion Concentration; D008297:Male; D015394:Molecular Structure; D010710:Phosphates / Q000737:chemistry; D011786:Quality Control; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D015203:Reproducibility of Results; D013267:Stilbenes / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D013429:Sulfatases / Q000494:pharmacology; D053719:Tandem Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D013696:Temperature; D014867:Water / Q000737:chemistry
D000097:Acetonitriles; D001952:Bridged-Ring Compounds; D002021:Buffers; D004304:Dosage Forms; D020719:Glucuronides; D010710:Phosphates; D013267:Stilbenes; C529889:plagiochin E; D014867:Water; D013429:Sulfatases; D005966:Glucuronidase; C032159:acetonitrile