Quantification of cysteine S-conjugate of 3-sulfanylhexan-1-ol in must and wine of petite arvine vine by stable isotope dilution analysis.
Making use of a convenient synthetic approach to prepare the deuterated S-3-(hexan-1-ol)-cysteine by a Michael addition reaction, an analytical method was developed to measure the presence of the cysteine S-conjugate, precursor of 3-sulfanylhexan-1-ol (3-mercaptohexan-1-ol), in must and wine from Petite Arvine vine. The method uses a stable isotope dilution assay with a suitable one-step sample preparation and HPLC-MS detection. The method has limits of detection and quantification of 3 and 10 microg/L, respectively. A correlation between the increase of the precursor concentration and the increase of the degree of rot has been established.
Luisier
Jean-Luc
JL
Institute of Life Technologies, University of Applied Sciences Western Switzerland Valais, Route de Rawyl 64, CH-1950 Sion 2, Switzerland.
Buettner
Hermann
H
Völker
Sebastian
S
Rausis
Thierry
T
Frey
Urban
U
D003545:Cysteine / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism*; D003903:Deuterium; D005285:Fermentation; D005638:Fruit / Q000737:chemistry*; D000441:Hexanols / Q000032:analysis* / Q000378:metabolism; D007201:Indicator Dilution Techniques; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000378:metabolism; D027843:Vitis / Q000737:chemistry*; D014920:Wine / Q000032:analysis*
D000441:Hexanols; C528100:S-3-(hexan-1-ol)cysteine; D013438:Sulfhydryl Compounds; D003903:Deuterium; D003545:Cysteine; C477019:3-mercaptohexanol