Herpesvirus saimiri transformation of human T lymphocytes.
Viral transformation of T cells is an effective method for obtaining large numbers of T cells that are easily maintained in the laboratory. This unit describes a method for generating antigen-independent, virally-transformed T cells using a T-lymphotropic primate gamma-2 herpesvirus, Herpesvirus saimiri (HVS; strain C488). Support protocols for preparing and titrating HSV C488 stocks and testing the functional status of transformed T cells are also included.
Fleckenstein
Bernhard
B
Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Ensser
Armin
A
Institut für Klinische und Molekulare Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
D002472:Cell Transformation, Viral / Q000276:immunology; D006567:Herpesvirus 2, Saimiriine / Q000276:immunology; D006801:Humans; D007158:Immunologic Techniques; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000821:virology; D014773:Virology / Q000379:methods