Generation of Epstein-Barr Virus (EBV)-immortalized B cell lines.
Immortalization of B lymphocytes by EBV is an effective procedure for inducing long-term growth of certain human B lymphocytes. The protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and EBV infection and growth of infected cells.
Tosato
Giovanna
G
National Cancer Institute, Bethesda, Maryland.
Cohen
Jeffrey I
JI
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology* / Q000821:virology; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral; D004854:Herpesvirus 4, Human / Q000502:physiology*; D006801:Humans