Comparative bioinformatics for mouse and human genes: getting started.
The primary goal of this unit is to describe methods for using online bioinformatic resources to identify data pertaining to specific mouse genes or genomic regions, from the perspective of a researcher who has identified a human gene(s) of interest and wishes to obtain some basic information about the orthologous murine gene-such as the mouse genomic sequence containing the gene, cDNA sequence and exon/intron structure, and conserved noncoding regions that may indicate regulatory elements. Basic Protocol 1 describes how to obtain mouse gene sequences via Ensembl. Basic Protocol 2 describes a sequence homology search for locating a mouse ortholog of a human gene using the UCSC genome browser. Basic Protocols 3 and 4 provide instructions for performing comparative analysis of large human and mouse genomics sequences, using the PipMaker (Schwartz et al., 2000) and VISTA (Mayor et al., 2000) online tools.
Mortlock
Douglas P
DP
Vanderbilt University Medical Center, Nashville, Tennessee, USA.
D000818:Animals; D019295:Computational Biology; D030541:Databases, Genetic; D005091:Exons; D005796:Genes; D006801:Humans; D051379:Mice; D009154:Mutation