title: Autoimmune ovarian disease induced by immunization with zona pellucida (ZP3) peptide.
abstract: Autoimmune ovarian disease is a known cause of human premature ovarian failure. An experimental murine model can be created by immunization with a peptide of ZP3, an ovary-specific glycoprotein. Mice injected with the ZP3 peptide develop histological evidence of ovarian inflammation (oophoritis), as well as antibody to the zona pellucida and T cell responses to the peptide. This unit describes the immunologic properties of the ZP3 peptides, the method of active autoimmune oophoritis induction, and the evaluation and semiquantitation of ovarian pathology. Surgical manipulations of the ovaries have been instrumental in the novel application of this autoimmune model; thus the most useful operations are also detailed. Surgical procedures are included for adult oophrectomy, neonatal ovariectomy, and implantation of ovarian grafts into mice without endogenous ovaries.
authors.affiliation: University of Virginia, Charlottesville, Virginia, USA.
authors.forename: Kenneth
authors.initials: K
authors.lastname: Tung
authors.forename: Sally
authors.initials: S
authors.lastname: Agersborg
authors.forename: Harini
authors.initials: H
authors.lastname: Bagavant
authors.forename: Kristine
authors.initials: K
authors.lastname: Garza
authors.forename: Kevin
authors.initials: K
authors.lastname: Wei
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004527:Egg Proteins / Q000276:immunology*; D005260:Female; D007114:Immunization; D008562:Membrane Glycoproteins / Q000276:immunology*; D051379:Mice; D010049:Ovarian Diseases / Q000276:immunology* / Q000473:pathology; D010052:Ovariectomy; D010053:Ovary / Q000637:transplantation; D011956:Receptors, Cell Surface / Q000276:immunology*; D019737:Transplants; D000072216:Zona Pellucida Glycoproteins
chemical_list: D004527:Egg Proteins; D008562:Membrane Glycoproteins; D011956:Receptors, Cell Surface; C000606517:ZP3 protein, human; D000072216:Zona Pellucida Glycoproteins; C000606521:Zp3 protein, mouse