A three-fragment phenotype in the D16S309 locus.
An allele with internal restriction site or another kind of mutation has been found in D16S309 locus. Using a probe MS205 in combination with the enzyme Hinf I 3-fragment, a pattern was observed in a woman from the Upper Silesia population--the mother of a child involved in paternity testing. DNA from this woman was additionally digested with Alu I and Mbo I enzymes. All the digested samples showed a 3-fragment pattern with the probe MS205, while other probe/enzyme combinations detected only the expected phenotypes. The disputed child inherited a second, normal allele from its mother.
Raczek
Ewa
E
Department of Forensic Medicine, Silesian Academy of Medicine, Katowice. medsad@slam.katowice.pl
Nowicka
Leokadia
L
D000483:Alleles; D002648:Child; D004247:DNA / Q000032:analysis*; D016172:DNA Fingerprinting / Q000379:methods*; D004252:DNA Mutational Analysis / Q000379:methods; D004262:DNA Restriction Enzymes / Q000235:genetics*; D005819:Genetic Markers; D005828:Genetics, Population; D006801:Humans; D008297:Male; D018598:Minisatellite Repeats / Q000235:genetics*; D010334:Paternity; D016133:Polymerase Chain Reaction
D005819:Genetic Markers; D004247:DNA; D004262:DNA Restriction Enzymes