Large-scale BAC clone restriction digest fingerprinting.
Restriction digest fingerprinting is a common method for characterizing large insert genomic clones, e.g., bacterial artificial chromosome (BAC), P1 artificial chromosome (PAC) and Fosmid clones. This clone fingerprinting method has been widely applied in the construction of clone-based physical maps, which have been used as positional cloning resources as well as to support directed and genome-wide sequencing efforts. This unit describes a robust, large-scale procedure for generation of agarose gel-based clone fingerprints from BAC clones.
Mathewson
Carrie A
CA
Canada's Michael Smith Genome Sciences Center Vancouver, British Columbia, Canada.
Schein
Jacqueline E
JE
Marra
Marco A
MA
D022202:Chromosomes, Artificial, Bacterial; D016172:DNA Fingerprinting / Q000379:methods*; D004262:DNA Restriction Enzymes; D004587:Electrophoresis, Agar Gel; D008722:Methods
D004262:DNA Restriction Enzymes