title: Isolation of murine hematopoietic stem cells and progenitor cells.
abstract: Protocols for the purification of CD34-KSL cells, CD34+KSL cells, SP cells, and Tip-SP CD34-KSL cells are described. Mouse hematopoietic stem cells are highly enriched in the CD34-KSL and SP fractions. These two populations overlap one another. A nearly homogenous population of HSCs can be obtained by isolating Tip-SP CD34-KSL cells. One of the earliest progenitor cell populations is CD34+KSL cells.
authors.affiliation: The University of Tokyo, Tokyo, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Ema
authors.forename: Yohei
authors.initials: Y
authors.lastname: Morita
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Nakauchi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Matsuzaki
mesh_terms: D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology*; D019070:Cell Lineage; D002469:Cell Separation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D018952:Antigens, CD34