Incorporation of halogenoalkyl, 2-pyridyldithioalkyl, or isothiocyanate linkers into ligands.
Ligands can be introduced at the 5' terminus of an oligonucleotide by adding a linker to the ligand and modifying the 5' terminus of the oligonucleotide. These are then reacted to give the ligand-oligonucleotide conjugate. The addition of appropriate linkers to ligands is described in this unit. 5'Modification of the oligonucleotide and the final reaction that produces the ligand-conjugated oligonucleotide are described elsewhere in the series. This approach is particularly useful when there is a limited amount of ligand available, when the ligand is sensitive to chemical conditions required for oligonucleotide deprotection, or when the ligand is weakly soluble in solvents required for phosphoramidite- or H-phosphonate-mediated oligonucleotide synthesis.
Asseline
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Centre de Biophysique Moléculaire, CNRS, Orléans, France.
Thuong
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D000166:Acridines / Q000737:chemistry; D001671:Biochemistry / Q000379:methods*; D005363:Ficusin / Q000737:chemistry; D006846:Hydrocarbons, Halogenated / Q000737:chemistry*; D017879:Isothiocyanates / Q000737:chemistry*; D008024:Ligands; D010618:Phenanthrolines / Q000737:chemistry; D011725:Pyridines / Q000737:chemistry*; D013844:Thiazoles / Q000737:chemistry
D000166:Acridines; D006846:Hydrocarbons, Halogenated; D017879:Isothiocyanates; D008024:Ligands; D010618:Phenanthrolines; D011725:Pyridines; D013844:Thiazoles; C037152:isothiocyanic acid; D005363:Ficusin