title: In vitro selection of RNA aptamers to a protein target by filter immobilization.
abstract: This unit describes the selection of aptamers from a pool of single-stranded RNA by binding to a protein target. Aptamers generated from this selection experiment can potentially function as protein inhibitors, and may find applications as therapeutic or diagnostic reagents. A pool of dsDNA is used to generate a ssRNA pool, which is mixed with the protein target. Bound complexes are separated from unbound reagents by filtration, and the RNA:protein complexes are amplified by a combination of reverse transcription, PCR, and in vitro transcription.
authors.affiliation: University of Texas, Austin, Texas, USA.
authors.forename: S D
authors.initials: SD
authors.lastname: Jhaveri
authors.forename: A D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000235:genetics / Q000302:isolation & purification*; D002417:Cattle; D005374:Filtration / Q000379:methods*; D016133:Polymerase Chain Reaction; D014176:Protein Biosynthesis; D011506:Proteins / Q000378:metabolism*; D052156:SELEX Aptamer Technique / Q000379:methods*; D014158:Transcription, Genetic
chemical_list: D052157:Aptamers, Nucleotide; D011506:Proteins