title: Growth and manipulation of yeast.
abstract: This unit describes preparation of selected media for growing yeast and also discusses strain storage and revival. Protocols are provided for the assay of beta-galactosidase in liquid culture and for transformation using lithium acetate.
authors.affiliation: Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Treco
authors.forename: A
authors.initials: A
authors.lastname: Reynolds
authors.forename: V
authors.initials: V
authors.lastname: Lundblad
mesh_terms: D000085:Acetates; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D014170:Transformation, Genetic; D001616:beta-Galactosidase
chemical_list: D000085:Acetates; D003470:Culture Media; D001616:beta-Galactosidase; C488804:lithium acetate