Measurement of TGF-beta in biological fluids.
Transforming growth factor (TGF-beta) is an immunoregulatory cytokine produced by many different cell types, including lymphocytes and monocytes-macrophages. Two assays are described for the quantitation of TGF-beta activity in supernatant fluids. The bioassay for TGF-beta is based on the potent ability of TGF-beta to suppress lymphocyte proliferation. The radioreceptor assay is based on the ability of TGF-beta-containing supernatant fluids to inhibit the binding of radiolabeled TGF-beta to its cellular receptor. Two support protocols describe acid-activation and neutralization of TGF-beta.
Hale-Donze
H
H
National Institute of Dental and Craniofacial Research, Bethesda, Maryland, USA.
Wahl
S M
SM
Jackson
R J
RJ
McGhee
J R
JR
D000818:Animals; D000906:Antibodies / Q000276:immunology; D001681:Biological Assay / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D008213:Lymphocyte Activation; D051379:Mice; D008863:Microspheres; D009500:Neutralization Tests / Q000379:methods; D011869:Radioligand Assay / Q000379:methods*; D018125:Receptors, Transforming Growth Factor beta / Q000378:metabolism; D011994:Recombinant Proteins / Q000032:analysis; D016212:Transforming Growth Factor beta / Q000032:analysis* / Q000276:immunology
D000906:Antibodies; D018125:Receptors, Transforming Growth Factor beta; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta