Staining proteins in gels.
This unit describes protocols for detecting protein in a gel by either Coomassie blue, silver or fluorescent staining. Alternate rapid staining procedures are provided for each method and a support protocol describes how to photograph stained gels. Fluorescent staining (e.g., with SYPRO Orange or Red) is described as a popular alternative to traditional staining procedures, mainly because it is more sensitive than Coomassie staining, and often as sensitive as silver staining. Staining of proteins in SDS-polyacrylamide gels is described, and an alternate protocol details variations in the procedure for proteins in nondenaturing gels. A final support protocol describes the photography of fluorescently stained proteins.
Sasse
Joachim
J
Shriners Hospital for Crippled Children, Tampa, Florida, USA.
Gallagher
Sean R
SR
D000818:Animals; D004396:Coloring Agents / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006801:Humans; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D013194:Staining and Labeling / Q000379:methods*
D004396:Coloring Agents; D011506:Proteins