A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain.
Inhibition of terminal respiratory oxidases by nitric oxide (NO) plays important physiological roles in signaling and host defense. Using a bacterial quinol oxidase and mitochondrial cytochrome c oxidase, this chapter describes simple polarographic methods to quantify the kinetic characteristics of inhibition by NO. This chapter points out the inherent pitfalls of both experimental design and data analysis and compares alternative methods. Additionally, it describes a system designed to acquire polarographic and spectral data simultaneously to permit identification of spectral intermediates under defined conditions.
Mason
Maria G
MG
Department of Biological Sciences, University of Essex, Colchester, United Kingdom.
Holladay
Rebecca S
RS
Nicholls
Peter
P
Shepherd
Mark
M
Cooper
Chris E
CE
D015374:Biosensing Techniques / Q000295:instrumentation; D004579:Electron Transport / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D020128:Inhibitory Concentration 50; D008954:Models, Biological; D009569:Nitric Oxide / Q000493:pharmacokinetics / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000032:analysis / Q000494:pharmacology; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D010100:Oxygen / Q000378:metabolism / Q000493:pharmacokinetics
D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D010088:Oxidoreductases; D010100:Oxygen