title: Chromatographic analysis of amino and organic acids in physiological fluids to detect inborn errors of metabolism.
abstract: This unit describes methods for the preparation of samples for analysis of physiological amino acids and organic acids. Amino acids are analyzed by ion-exchange chromatography using an automated system. Organic acids are analyzed by gas-chromatography/mass spectrometry (GC-MS). Analysis of amino and organic acids is necessary to detect and monitor the treatment of many inborn errors of metabolism.
authors.affiliation: University of Colorado Health Sciences Center, Aurora, Colorado, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Woontner
authors.forename: Stephen I
authors.initials: SI
authors.lastname: Goodman
mesh_terms: D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D001826:Body Fluids / Q000737:chemistry* / Q000502:physiology; D002264:Carboxylic Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008661:Metabolism, Inborn Errors / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism
chemical_list: D000596:Amino Acids; D002264:Carboxylic Acids