title: Evaluation of circulating cathodic antigen (CCA) strip for diagnosis of urinary schistosomiasis in Hassoba school children, Afar, Ethiopia.
abstract: A total of 206 urine samples collected from Hassoba Elementary schoolchildren, Afar, Ethiopia, a low Schistosoma haematobium endemic setting, was diagnosed to evaluate the performance of CCA strip using double references, urine filtration technique and urinalysis dipstick (Combur 1.0 Test) that detect schistosome eggs and blood in urine, respectively. The former was used as a gold standard reference method. Sensitivity, specificity, positive and negative predictive values for the CCA were 52%, 63.8%, 56.7% and 59% respectively, with reference to urine filtration technique whereas these parameters were 50.4%, 62.4%, 55.6% and 57.5% respectively, with reference to Combur 10 Test. 47 S. haematobium egg-positive children were found negative by CCA strip while 38 egg-negative children were found positive by CCA strip. Moreover, among the pre-tests done in duplicate, inconsistent results were also recorded. Assays were also compared with regard to the cost of equipment and reagents, speed and simplicity of use. Though CCA strip was found to be rapid and could be performed with minimal training, it was found to be expensive (US $ 4.95 per test) to use it for large-scale field use even if its diagnostic value would have been satisfactory. Further development and standardization of the CCA strip are required for its applicability for field use. It is also recommended that its cost per strip should be substantially cut down if it is to be used in poor schistosomiasis endemic countries.
authors.affiliation: Carter Center-Ethiopia, P.O. Box 13373, Addis Ababa, Ethiopia.
authors.forename: B
authors.initials: B
authors.lastname: Ayele
authors.forename: B
authors.initials: B
authors.lastname: Erko
authors.forename: M
authors.initials: M
authors.lastname: Legesse
authors.forename: A
authors.initials: A
authors.lastname: Hailu
authors.forename: G
authors.initials: G
authors.lastname: Medhin
mesh_terms: D000293:Adolescent; D000328:Adult; D000818:Animals; D000947:Antigens, Helminth / Q000652:urine*; D002648:Child; D002675:Child, Preschool; D003365:Costs and Cost Analysis; D005002:Ethiopia / Q000453:epidemiology; D005260:Female; D005374:Filtration; D006023:Glycoproteins; D015801:Helminth Proteins / Q000652:urine*; D006801:Humans; D008297:Male; D010270:Parasite Egg Count; D011237:Predictive Value of Tests; D011934:Reagent Strips / Q000191:economics; D012548:Schistosoma haematobium / Q000302:isolation & purification*; D012553:Schistosomiasis haematobia / Q000175:diagnosis* / Q000453:epidemiology / Q000652:urine; D012680:Sensitivity and Specificity
chemical_list: D000947:Antigens, Helminth; C091532:CCA protein, Schistosoma mansoni; D006023:Glycoproteins; D015801:Helminth Proteins; D011934:Reagent Strips