title: Delayed-type hypersensitivity.
abstract: Delayed-type hypersensitivity (DTH) is an in vivo assay of cell-mediated immune function. DTH reactions are often divided into two phases: the sensitization phase, referring to the initial immunization with specific antigen, and the efferent or challenge phase of the DTH response, which usually follows 6 to 14 days after sensitization. This unit details the protocol for stimulating DTH responses in mice to the O-succinimide ester of the hapten 4-hydroxy-3-nitrophenyl acetyl (abbreviated NP-O-Su).
authors.affiliation: Harvard Medical School, Boston, Massacusetts, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Luo
authors.forename: M E
authors.initials: ME
authors.lastname: Dorf
mesh_terms: D000818:Animals; D006241:Haptens / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D051379:Mice; D009596:Nitrophenols / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology
chemical_list: D006241:Haptens; D009596:Nitrophenols; C026654:4-hydroxy-3-nitrophenylacetyl-O-succinimide ester