Electrochemiluminescence immunosensor based on CdSe nanocomposites.
A novel strategy for the enhancement of electrochemiluminescence (ECL) was developed by combining CdSe nanocrystals (NCs), carbon nanotube-chitosan (CNT-CHIT), and 3-aminopropyl-triethoxysilane (APS). A label-free ECL immunosensor for the sensitive detection of human IgG (HIgG) was fabricated. The colloidal solution containing CdSe NCs/CNT-CHIT composite was first covered on the Au electrode surface to form a robust film, which showed high ECL intensity and good biocompatibility. After APS as a cross-linker was covalently conjugated to the CdSe NCs/CNT-CHIT film, the ECL intensity was greatly enhanced. And, an intensity about 20-fold higher than that of the CdSe NCs/CNT-CHIT film was observed. After antibody was bound to the functionalized film via glutaric dialdehyde (GLD), the modified electrode could be used as an ECL immunosensor for the detection of HIgG. The specific immunoreaction between HIgG and antibody resulted in the decrease in ECL intensity. The ECL intensity decreased linearly with HIgG concentration in the range of 0.02-200 ng mL(-1), and the detection limit was 0.001 ng mL(-1). The immunosensor has the advantages of high sensitivity, speed, specificity, and stability and could become a promising technique for protein detection.
Jie
Guifen
G
Key Laboratory of Analytical Chemistry for Life Science, Ministry of Education of China, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
Zhang
Jingjing
J
Wang
Danchen
D
Cheng
Chao
C
Chen
Hong-Yuan
HY
Zhu
Jun-Jie
JJ
D000042:Absorption; D000918:Antibody Specificity; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D019187:Cadmium Compounds / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis / Q000276:immunology; D008163:Luminescent Measurements / Q000379:methods*; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D011437:Propylamines; D015203:Reproducibility of Results; D018036:Selenium Compounds / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry; D013818:Thermogravimetry
D001672:Biocompatible Materials; D019187:Cadmium Compounds; D007074:Immunoglobulin G; D037742:Nanotubes, Carbon; D011437:Propylamines; D018036:Selenium Compounds; D012821:Silanes; D048271:Chitosan; C058667:cadmium selenide; C477625:amino-propyl-triethoxysilane