[Determination of emamectin benzoate residue in vegetables by high performance liquid chromatography with fluorescence detection].
A method was developed for the determination of emamectin benzoate residue in cabbage and mushroom using solid-phase extraction (SPE) and high performance liquid chromatography (HPLC) with fluorescence detection. The sample was extracted with ethyl acetate. Further cleanup was performed on a propylsulfonic acid solid phase extraction cartridge, followed by the derivatization with trifluoroacetic anhydride in the presence of N-methylimidazole. The amount of derivatized emamectin benzoate was determined by fluorescence detector after separation by HPLC. The detection limit was 0.10 microg/kg for cabbage and mushroom samples. The recoveries of emamectin benzoate in cabbage and mushroom samples were 78.6%-84.9%. The inter-day relative standard deviation (RSD) and intra-day RSD were 2.7%-6.0% and 3.1%-8.9%, respectively, at the fortified levels of 1.0-20.0 microg/kg. The calibration curve of emamectin benzoate in vegetables at the concentration range of 0.002 mg/L to 0.10 mg/L was linear (r = 0.9999).
Zhang
Yan
Y
Beijing Huadu Broiler Corporation, Beijing, China. zhangsky001@tom.com
Wu
Yinliang
Y
Hu
Jiye
J
Wang
Hongwei
H
Pan
Canping
C
Liu
Fengmao
F
D000363:Agaricales / Q000737:chemistry*; D001937:Brassica / Q000737:chemistry*; D005504:Food Analysis / Q000379:methods*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis / Q000302:isolation & purification; D057230:Limit of Detection; D016014:Linear Models; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013050:Spectrometry, Fluorescence
D010573:Pesticide Residues; D012997:Solvents; D007559:Ivermectin; C108024:emamectin benzoate