title: Occurrence and genotype characterization of Giardia duodenalis in goat kids from the Canary Islands, Spain.
abstract: Giardia duodenalis (syn. Giardia lamblia, Giardia intestinalis) is a wide-spread intestinal protozoa of both humans and animals. Although giardiosis in goat is commonly asymptomatic, young kids may bear an enteric disease associated with persistent diarrhoea and delayed weight gain. In the present study we have analysed the occurrence of Giardia in 315 young goat kids (2-6 months old) from Gran Canaria Island (Spain) through visualization of faecal cysts. The identification of genotypes of G. duodenalis among the farms was attained by nested PCR of the triophosphate isomerase (TPI) and single PCR of beta-giardin genes and subsequent sequencing. Positive samples were found in 42.2% of the animals and 95.5% of the farms. Goat faecal specimens were positive for only livestock-associated G. duodenalis assemblage E genotype for both TPI and beta-giardin genes. The genetic analysis of these two loci revealed the presence of different haplotypes among the farms included in the survey and high homology with homologous genes from cattle and sheep. Altogether, the data presented here provide additional information to the prevalence and genetic characterization of Giardia isolates. The absence of assemblages A and B in this study suggests that zoonotic transmission of Giardia from goats could be of low epidemiological significance, although these findings should be validated in studies including other geographical areas, age groups and larger number of samples.
authors.affiliation: Parasitology Unit, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, Trasmontaña s/n, 35413 Arucas, Spain. aruiz@dpat.ulpgc.es
authors.forename: Antonio
authors.initials: A
authors.lastname: Ruiz
authors.forename: Pilar
authors.initials: P
authors.lastname: Foronda
authors.forename: Jorge F
authors.initials: JF
authors.lastname: González
authors.forename: Aránzazu
authors.initials: A
authors.lastname: Guedes
authors.forename: Néstor
authors.initials: N
authors.lastname: Abreu-Acosta
authors.forename: José M
authors.initials: JM
authors.lastname: Molina
authors.forename: Basilio
authors.initials: B
authors.lastname: Valladares
mesh_terms: D000818:Animals; D005838:Genotype; D005872:Giardia / Q000235:genetics*; D005873:Giardiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D015511:Goat Diseases / Q000453:epidemiology / Q000469:parasitology*; D006041:Goats; D010802:Phylogeny; D013030:Spain / Q000453:epidemiology