3'-modified oligonucleotides and their conjugates.
Solid-phase synthesis of 3'-aminoalkyl, 3'-thioalkyl, and 3'-polyethyleneglycol are described. The first two are important as specific points of attachment for a large variety of reporter groups; the latter is important because of its increased cell membrane permeability, which aids in the development of antisense drugs. These protocols cover details of the synthetic organic chemistry needed to make each solid support, as well as DNA synthesis, workup, characterization, and conjugation.
Lyttle
M H
MH
Biosearch Technologies, Inc., Novato, California, USA.
D000478:Alkylation; D000812:Anhydrides / Q000737:chemistry; D001671:Biochemistry / Q000379:methods*; D004247:DNA / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000812:Anhydrides; D009841:Oligonucleotides; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D004247:DNA