Proteomic and metabolomic analyses of the white-rot fungus Phanerochaete chrysosporium exposed to exogenous benzoic acid.
Intracellular processes of the white-rot basidiomycete Phanerochaete chrysosporium involved in the metabolism of benzoic acid (BA) were investigated at the proteome and metabolome level. Up-regulation of aryl-alcohol dehydrogenase, arylaldehyde dehydrogenase, and cytochrome P450s was observed upon addition of exogenous BA, suggesting that these enzymes play key roles in its metabolism. Intracellular metabolic shifts from the short-cut TCA/glyoxylate bicycle system to the TCA cycle and an increased flux in the TCA cycle indicated activation of the heme biosynthetic pathway and the production of NAD(P)H. In addition, combined analyses of proteome and metabolome clearly indicated the role of trehalose as a storage disaccharide and that the mannitol cycle plays a role in an alternative energy-producing pathway.
Matsuzaki
Fumiko
F
Faculty of Agriculture, Bio-Architecture Center, Kyushu University, 6-10-1, Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan.
Shimizu
Motoyuki
M
Wariishi
Hiroyuki
H
D000429:Alcohol Oxidoreductases / Q000378:metabolism; D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D000596:Amino Acids / Q000378:metabolism; D019817:Benzoic Acid / Q000494:pharmacology*; D050260:Carbohydrate Metabolism / Q000187:drug effects; D002952:Citric Acid Cycle / Q000187:drug effects; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects; D015180:Electrophoresis, Gel, Two-Dimensional; D005656:Fungal Proteins / Q000032:analysis / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D006019:Glycolysis / Q000187:drug effects; D006360:Heat-Shock Proteins / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D009249:NADP / Q000378:metabolism; D020075:Phanerochaete / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014199:Trehalose / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
D000596:Amino Acids; D005656:Fungal Proteins; D006360:Heat-Shock Proteins; D020543:Proteome; D009243:NAD; D009249:NADP; D019817:Benzoic Acid; D003577:Cytochrome P-450 Enzyme System; D014199:Trehalose; D000429:Alcohol Oxidoreductases; C055882:aryl-alcohol dehydrogenase; D000445:Aldehyde Oxidoreductases; C116588:aryl-aldehyde oxidoreductase