title: Certification and uncertainty evaluation of the certified reference materials of poly(ethylene glycol) for molecular mass fractions by using supercritical fluid chromatography.
abstract: Poly(ethylene glycol) (PEG) is a useful water-soluble polymer that has attracted considerable interest in medical and biological science applications as well as in polymer physics. Through the use of a well-calibrated evaporative light-scattering detector coupled with high performance supercritical fluid chromatography, we are able to determine exactly not only the average mass but also all of the molecular mass fractions of PEG samples needed for certified reference materials issued by the National Metrology Institute of Japan. In addition, experimental uncertainty was determined in accordance with the Guide to the expression of uncertainty in measurement (GUM). This reference material can be used to calibrate measuring instruments, to control measurement precision, and to confirm the validity of measurement methods when determining molecular mass distributions and average molecular masses. Especially, it is suitable for calibration against both masses and intensities for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
authors.affiliation: Materials Characterization Division, National Metrology Institute of Japan, Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan. kayori.takahashi@ni.aist.go.jp
authors.forename: Kayori
authors.initials: K
authors.lastname: Takahashi
authors.forename: Kana
authors.initials: K
authors.lastname: Kishine
authors.forename: Shigetomo
authors.initials: S
authors.lastname: Matsuyama
authors.forename: Takeshi
authors.initials: T
authors.lastname: Saito
authors.forename: Haruhisa
authors.initials: H
authors.lastname: Kato
authors.forename: Shinichi
authors.initials: S
authors.lastname: Kinugasa
mesh_terms: D002138:Calibration; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D007564:Japan; D008970:Molecular Weight; D011092:Polyethylene Glycols / Q000592:standards*; D012015:Reference Standards
chemical_list: D011092:Polyethylene Glycols