title: Effect of cleansers and irrigation methods on primary root dentin permeability.
abstract: OBJECTIVE
  The purpose of this study was to evaluate the effects of cleansers and irrigation methods on the permeability index (PI) in primary root dentin.
  
  
  METHODS
  One hundred twelve teeth were extracted and sectioned transversely, discarding their crowns. Then, the roots were distributed according to the cleansers: (1) Dakin (D); (2) Dakin+hydrogen peroxide (DHP); (3) 2% chlorhexidine gel (C); and (4) saline (S). The canals were manually instrumented. Irrigation was performed manually (MI) or ultrasonically (UI). The roots were: (1) made impermeable; (2) filled with 2% methylene blue solution; and (3) longitudinally sectioned. The halves were marked in thirds (cervical, middle, and apical), and the areas of dye penetration were measured. PI data were submitted to factorial analysis of variance and Tukey tests (P<.05).
  
  
  RESULTS
  A significant effect of MI/UI, in association with cleansers, was seen on PI. For all thirds, the PI of MI was superior to UI. In the cervical third, MI achieved a high PI when associated with D, DHP, and S. UI associated with S and C produced the highest PI. In the middle third, MI associated with D and DHP produced the highest PI. In the apical third, MI associated with D demonstrated the highest PI. None of the UI and cleanser associations induced differences in PI for the middle and apical thirds.
  
  
  CONCLUSIONS
  MI produced better results than the UI. The D, DHP, and S cleansers demonstrated the highest PIs, suggesting that the combination of MI and these cleansers is recommended for primary teeth to maintain dentin permeability.
authors.affiliation: Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.
authors.forename: Fernanda Miori
authors.initials: FM
authors.lastname: Pascon
authors.forename: Kamila Rosamilia
authors.initials: KR
authors.lastname: Kantovitz
authors.forename: Ana Flávia Sanches
authors.initials: AF
authors.lastname: Borges
authors.forename: Regina Maria
authors.initials: RM
authors.lastname: Puppin-Rontani
mesh_terms: D000704:Analysis of Variance; D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D002710:Chlorhexidine / Q000494:pharmacology; D019551:Dental Disinfectants / Q000494:pharmacology*; D003786:Dental Pulp Cavity / Q000187:drug effects; D003806:Dentin Permeability / Q000187:drug effects*; D004338:Drug Combinations; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D018915:Root Canal Preparation / Q000379:methods; D016369:Smear Layer; D017693:Sodium Bicarbonate / Q000494:pharmacology; D012973:Sodium Hypochlorite / Q000494:pharmacology; D014094:Tooth, Deciduous / Q000187:drug effects*; D014465:Ultrasonics
chemical_list: D000891:Anti-Infective Agents, Local; D019551:Dental Disinfectants; D004338:Drug Combinations; D012388:Root Canal Irrigants; C069467:modified Dakin's solution; D017693:Sodium Bicarbonate; D006861:Hydrogen Peroxide; D012973:Sodium Hypochlorite; D002710:Chlorhexidine