Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber.
The optothermal properties of nanoparticles are of interest for biosensors and highly sensitive biochip applications. In this respect, the longitudinal resonance of Au nanorods was used to transform near infrared energy into thermal energy in a microfluidic chip. The resulting heat generated effectively caused pathogen lysis. Consequently the DNA was extracted out of the cell body and transferred to a PCR system. This resulted in the successful demonstration of a one step real-time PCR system for pathogen detection without removal or changing of reagents.
Cheong
Kwang Ho
KH
Bio & Health Lab, Samsung Advanced Institute of Technology, Giheung, Korea. kwangho.cheong@samsung.com
Yi
Dong Kee
DK
Lee
Jeong-Gun
JG
Park
Jong-Myeon
JM
Kim
Min Jun
MJ
Edel
Joshua B
JB
Ko
Christopher
C
D015374:Biosensing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D006046:Gold / Q000737:chemistry*; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D046208:Microchip Analytical Procedures / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
D004269:DNA, Bacterial; D006046:Gold