Direct attachment of conjugate groups to the 5' terminus of oligodeoxyribonucleotides.
This unit gives protocols for the attachment of intercalating and photoreactive conjugate groups to oligodeoxyribonucleotides. Protocols are given for acridine- and psoralen-conjugated oligonucleotides, and include attachment of the linker, preparation of the phosphoramidite, coupling to the oligonucleotide, deprotection, purification, and characterization.
Asseline
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Centre de Biophysique Moléculaire, CNRS, Orléans, France.
Thuong
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D000166:Acridines / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D001671:Biochemistry / Q000379:methods*; D005363:Ficusin / Q000138:chemical synthesis / Q000737:chemistry; D006900:Hydroxylation; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000737:chemistry* / Q000302:isolation & purification; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry; D013057:Spectrum Analysis
D000166:Acridines; D009838:Oligodeoxyribonucleotides; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D005363:Ficusin