[Determination of exogenous hormones in plant tissue culture media by reversed-phase high performance liquid chromatography].
A method of high performance liquid chromatography coupled with photodiode array detector (PAD) was established for the determination of the content ratio and type of 6 exogenous hormones in plant tissue culture media. The column was a microBondaPak C18 (3.9 mm x 300 mm, 10 microm, Waters), the mobile phase was 140 mmol/L sodium acetate in triethyl amine buffer (pH 4.95)-acetonitrile (75:25, v/v), and the flow rate was 1.0 mL/min. The column temperature was 37 degrees C, and the detection wavelength was 285 nm. The six hormones reached the baseline separation in 9 minutes. The linear relationship was very good in the range of 4-200 ng (r2 > 0.9995). The exogenous hormones in the medium were extracted by methanol after vacuum dried. The average recoveries of the exogenous hormones were more than 85%. The method can be used for the analysis of exogenous hormones of plant tissue culture media, or of unknown hormone ratio and the type of media.
Chen
Yongbo
Y
The Konjak Research Institute of Enshi Autonomic State Academy of Agricultural Sciences, Enshi, China. yongboc@tom.com
Zhao
Qinghua
Q
Jiang
Qiaolong
Q
Teng
Jianxun
J
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D006728:Hormones / Q000032:analysis* / Q000302:isolation & purification; D057230:Limit of Detection; D016014:Linear Models; D059828:Plant Cells; D015203:Reproducibility of Results; D046509:Tissue Culture Techniques
D003470:Culture Media; D006728:Hormones