title: [Cloning and sequence analysis of envelope glycoprotein G2 gene of hantavirus in Shandong province].
abstract: OBJECTIVE
  To construct the cloning vector of glycoprotein G2 gene of hantavirus (HV), to analyze the sequence of G2 gene by the phylogenetic tree, and to study the differences among glycoprotein G2 genes from the world around.
  
  
  METHODS
  Envelope glycoprotein G2 gene was amplified from four specimens of Shandong province by RT-PCR, and the product recombined into the PMD-18T vector. The clones that carry the G2 gene were identified. After sequencing, the gene sequence was handled with the software DNASTAR, compared with 24 strains worldwide and the phylogenetic tree was drawn.
  
  
  RESULTS
  HV G2 gene was amplified by RT-PCR from 4 specimens, named GM04-38.G2, ZB8.G2, JUN5-14.G2, RCH5.G2, respectively. The map of the phylogenetic tree showed that all the 4 strains belonged to SEO-type hantavirus. The analysis of the sequence showed that all the four HV strains had the highest rates of homology with Z37 strain. The sequence homology of SEO-type HV strains was from 82.3% to 99.8%.
  
  
  CONCLUSIONS
  The four cloning vectors containing the glycoprotein G2 genes were successfully constructed. Envelope glycoprotein G2 gene of four specimens from Shandong province had high homology rates.
authors.affiliation: Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.
authors.forename: Shao-xia
authors.initials: SX
authors.lastname: Song
authors.forename: Zhi-yu
authors.initials: ZY
authors.lastname: Wang
authors.forename: Zhen-qiang
authors.initials: ZQ
authors.lastname: Bi
authors.forename: Zhi-qiang
authors.initials: ZQ
authors.lastname: Wang
authors.forename: Ze-xin
authors.initials: ZX
authors.lastname: Tao
authors.forename: Yu-lu
authors.initials: YL
authors.lastname: Wang
authors.forename: Yan-yan
authors.initials: YY
authors.lastname: Song
authors.forename: Gui-ting
authors.initials: GT
authors.lastname: Wang
authors.forename: Hong-zhi
authors.initials: HZ
authors.lastname: Xu
mesh_terms: D000818:Animals; D002681:China; D003001:Cloning, Molecular; D006476:Orthohantavirus / Q000235:genetics*; D051379:Mice; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
chemical_list: D014759:Viral Envelope Proteins; C104008:glycoprotein G1, Hantavirus