title: Generation and maintenance of cloned human T cell lines.
abstract: This unit describes protocols for the generation of human (allo-) antigen-specific T cell lines, and T lymphocyte subpopulations with distinct cytokine production profiles from purified peripheral or cord blood CD4+ T cells, respectively. Methods for the cloning and maintenance of these cell lines are given, as well as a protocol for freeze/thaw procedures.
authors.affiliation: INSERM U454, Montpellier, France.
authors.forename: Hans
authors.initials: H
authors.lastname: Yssel
authors.forename: Hergen
authors.initials: H
authors.lastname: Spits
mesh_terms: D002460:Cell Line; D003001:Cloning, Molecular; D015925:Cryopreservation; D016207:Cytokines / Q000276:immunology; D006801:Humans; D013601:T-Lymphocytes / Q000166:cytology* / Q000276:immunology
chemical_list: D016207:Cytokines