title: Histamine H(4) receptors regulate ACTH release in AtT-20 cells.
abstract: The early research described that adrenocorticotropic hormone (ACTH) release from mouse pituitary tumor AtT-20 cells was regulated by histamine H(3) receptors. Here, we provide the evidence that histamine H(4) receptor was expressed in AtT-20 cell, and that the accelerated ACTH secretions from the cells by histamine and R-alpha-methylhistamine were blocked by JNJ7777120, a specific H(4) receptor antagonist, in concentration dependent manner, but not by the H(1) and H(2) receptor antagonists. The results indicate, for the first time, that histamine H(4) receptor, rather than histamine H(3) receptor, played a role in regulation of ACTH release from mouse pituitary AtT-20 cells.
authors.affiliation: Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xian 710032, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Meng
authors.forename: Xue
authors.initials: X
authors.lastname: Ma
authors.forename: Mingkai
authors.initials: M
authors.lastname: Li
authors.forename: Min
authors.initials: M
authors.lastname: Jia
authors.forename: Xiaoxing
authors.initials: X
authors.lastname: Luo
mesh_terms: D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D006633:Histamine Antagonists / Q000494:pharmacology; D007211:Indoles / Q000494:pharmacology; D008761:Methylhistamines / Q000494:pharmacology; D051379:Mice; D010879:Piperazines / Q000494:pharmacology; D043562:Receptors, G-Protein-Coupled / Q000187:drug effects / Q000502:physiology*; D011968:Receptors, Histamine / Q000187:drug effects / Q000502:physiology*; D018100:Receptors, Histamine H3 / Q000187:drug effects; D000074040:Receptors, Histamine H4; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D006633:Histamine Antagonists; C498829:Hrh4 protein, mouse; D007211:Indoles; D008761:Methylhistamines; D010879:Piperazines; D043562:Receptors, G-Protein-Coupled; D011968:Receptors, Histamine; D018100:Receptors, Histamine H3; D000074040:Receptors, Histamine H4; C484309:1-((5-chloro-1H-indol-2-yl)carbonyl)-4-methylpiperazine; C069357:alpha-methylhistamine; D000324:Adrenocorticotropic Hormone