title: Hydroxylapatite chromatography.
abstract: Hydroxylapatite (also called hydroxyapatite), a form of calcium phosphate, can be used as a matrix for the chromatography of both proteins and nucleic acids. Protocols are provided for both standard low-pressure chromatography of a protein mixture using a hydroxylapatite column prepared in the laboratory, and an HPLC method, applicable to proteins and nucleic acids, that uses a commercially available column. Alternate protocols describe column chromatography using a step gradient or batch binding and step-gradient elution.
authors.affiliation: Roche Products Ltd., Welwyn Garden City, Hertfordshire, United Kingdom.
authors.forename: A V
authors.initials: AV
authors.lastname: Broadhurst
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D017886:Durapatite / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry / Q000302:isolation & purification; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D009696:Nucleic Acids; D011506:Proteins; D017886:Durapatite