Polychaetoid controls patterning by modulating adhesion in the Drosophila pupal retina.
Correct cellular patterning is central to tissue morphogenesis, but the role of epithelial junctions in this process is not well-understood. The Drosophila pupal eye provides a sensitive and accessible model for testing the role of junction-associated proteins in cells that undergo dynamic and coordinated movements during development. Mutations in polychaetoid (pyd), the Drosophila homologue of Zonula Occludens-1, are characterized by two phenotypes visible in the adult fly: increased sensory bristle number and the formation of a rough eye produced by poorly arranged ommatidia. We found that Pyd was localized to the adherens junction in cells of the developing pupal retina. Reducing Pyd function in the pupal eye resulted in mis-patterning of the interommatidial cells and a failure to consistently switch cone cell contacts from an anterior-posterior to an equatorial-polar orientation. Levels of Roughest, DE-Cadherin and several other adherens junction-associated proteins were increased at the membrane when Pyd protein was reduced. Further, both over-expression and mutations in several junction-associated proteins greatly enhanced the patterning defects caused by reduction of Pyd. Our results suggest that Pyd modulates adherens junction strength and Roughest-mediated preferential cell adhesion.
Seppa
Midori J
MJ
Department of Molecular Biology and Pharmacology, Washington University School of Medicine, 660 South Euclid Avenue, Saint Louis, MO 63110, USA.
Johnson
Ruth I
RI
Bao
Sujin
S
Cagan
Ross L
RL
D022005:Adherens Junctions / Q000378:metabolism*; D000818:Animals; D019521:Body Patterning; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002448:Cell Adhesion / Q000502:physiology*; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000235:genetics / Q000378:metabolism; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000033:anatomy & histology / Q000196:embryology / Q000378:metabolism; D005136:Eye Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D009024:Morphogenesis; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D017956:Photoreceptor Cells, Invertebrate / Q000166:cytology / Q000196:embryology; D011679:Pupa / Q000033:anatomy & histology* / Q000378:metabolism; D034622:RNA Interference; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012160:Retina / Q000166:cytology / Q000196:embryology; D015398:Signal Transduction / Q000502:physiology; D062725:Tight Junction Proteins; D014921:Wings, Animal / Q000033:anatomy & histology / Q000196:embryology; D062826:Zonula Occludens-1 Protein; D051178:alpha Catenin / Q000235:genetics / Q000378:metabolism
D015820:Cadherins; D015815:Cell Adhesion Molecules; D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D005136:Eye Proteins; D008565:Membrane Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D062725:Tight Junction Proteins; D062826:Zonula Occludens-1 Protein; D051178:alpha Catenin; C081439:alpha-Cat protein, Drosophila; C064196:dpp protein, Drosophila; C569041:pyd protein, Drosophila; C085209:rst protein, Drosophila; C501889:shg protein, Drosophila