Overview of Biacore systems and their applications.
Surface plasmon resonance (SPR) allows for the investigation of the functional nature of binding interactions and provides detailed kinetic information across a wide range of molecular weights, including small molecules, all without the use of labels. Here the various Biacore instrument platforms and their primary uses, ranging from semi-automated systems designed for simple, flexible basic research to fully automated, high-throughput systems, and systems designed to function in regulated environments, are all highlighted. The available sensor chip surfaces and immobilization techniques are also discussed. Biacore SPR biosensors can be used for a wide variety of assays, including specificity, active concentration measurement, kinetics, and affinity and thermodynamic parameters. Biacore SPR biosensors, which measure real-time analysis of biospecific interactions without the use of labeled molecules, can be used for a wide variety of protein interaction assays. In this unit, examples and recommendations for studying protein interactions with a variety of molecules are provided. This unit also shows how the technology can be used to determine binding specificity, active concentration measurements, and the determination of kinetic and thermodynamic parameters.
Jason-Moller
Laure
L
Biacore, Inc., Piscataway, New Jersey, USA.
Murphy
Michael
M
Bruno
JoAnne
J
D001331:Automation; D015374:Biosensing Techniques; D015195:Drug Design; D005504:Food Analysis; D007700:Kinetics; D044085:Microfluidics; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods*; D013816:Thermodynamics