Retinol and retinoic acid modulate catalase activity in Sertoli cells by distinct and gene expression-independent mechanisms.
Vitamin A (retinol) exerts a major role in several biological functions. However, it was observed that retinol induces oxidative stress on different cellular types. Catalase (EC 1.11.1.6; CAT) is a hydrogen peroxide metabolizing enzyme, and its activity and expression is widely used as an index to measure oxidative stress and perturbations in the cellular redox state. The aim of this study was to investigate the effects of retinol and its major biologically active metabolite, all-trans retinoic acid (RA), on CAT regulation. For this purpose, cultured Sertoli cells (a physiological target of vitamin A) were treated with retinol or RA. Retinol (7 microM, 14 microM) and RA (100 nM, 1 microM) enhanced intracellular reactive species production and increased CAT activity after 24 h of treatment. Retinol increased CAT immunocontent but did not alter CAT mRNA expression, while the increase in CAT activity by RA was not related to alterations in immunocontent or mRNA expression. In vitro incubation of purified CAT with retinol or RA did not alter enzyme activity.
Pasquali
Matheus A B
MA
CEEO, Centro de Estudos em Estresse Oxidativo, Departamento de Bioquímica, Laboratório 32, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2600 Anexo, CEP 90035003, Porto Alegre, RS, Brazil. 00124262@ufrgs.br
Gelain
Daniel P
DP
Zanotto-Filho
Alfeu
A
de Souza
Luiz F
LF
de Oliveira
Ramatis B
RB
Klamt
Fabio
F
Moreira
Jose C F
JC
D000818:Animals; D000970:Antineoplastic Agents / Q000633:toxicity*; D002374:Catalase / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D012708:Sertoli Cells / Q000187:drug effects* / Q000201:enzymology; D014212:Tretinoin / Q000633:toxicity*; D014801:Vitamin A / Q000633:toxicity*; D014815:Vitamins / Q000633:toxicity*
D000970:Antineoplastic Agents; D005609:Free Radicals; D012333:RNA, Messenger; D014815:Vitamins; D014801:Vitamin A; D014212:Tretinoin; D002374:Catalase