Quantitative expression patterns of peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) protein in mice.
The expression patterns of PPARbeta/delta have been described, but the majority of these data are based on mRNA data. To date, there are no reports that have quantitatively examined the expression of PPARbeta/delta protein in mouse tissues. In the present study, a highly specific PPARbeta/delta antibody was developed, characterized, and used to examine tissue expression patterns of PPARbeta/delta. As compared to commercially available anti-PPARbeta/delta antibodies, one of six polyclonal anti-PPARbeta/delta antibodies developed was significantly more effective for immunoprecipitation of in vitro-translated PPARbeta/delta. This antibody was used for quantitative Western blot analysis using radioactive detection methods. Expression of PPARbeta/delta was highest in colon, small intestine, liver, and keratinocytes as compared to other tissues including heart, spleen, skeletal muscle, lung, brain, and thymus. Interestingly, PPARbeta/delta expression was localized in the nucleus and RXRalpha can be co-immunoprecipitated with nuclear PPARbeta/delta. Results from these studies demonstrate that PPARbeta/delta expression is highest in intestinal epithelium, liver, and keratinocytes, consistent with significant biological roles in these tissues.
Girroir
Elizabeth E
EE
Department of Veterinary and Biomedical Sciences, The Center of Molecular Toxicology and Carcinogenesis, The Pennsylvania State University, 312 Life Sciences Building, University Park, PA 16802, USA.
Hollingshead
Holly E
HE
He
Pengfei
P
Zhu
Bokai
B
Perdew
Gary H
GH
Peters
Jeffrey M
JM
D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D047494:PPAR delta / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D048068:PPAR-beta / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D011817:Rabbits; D014018:Tissue Distribution
D000906:Antibodies; D047494:PPAR delta; D048068:PPAR-beta; D012333:RNA, Messenger