Enzymatic labeling of DNA.
This appendix describes the preparation of uniformly labeled radioactive probes by nick translation and random oligonucleotide-primed synthesis, as well as the end-labeling of oligonucleotides by T4 polynucleotide kinase. Accompanying these protocols are methods for removing unincorporated dNTP precursors with spin columns and for measuring the specific activity of a probe by acid precipitation.
Tabor
S
S
Harvard Medical School, Boston, Massachusetts, USA.
Struhl
K
K
D011232:Chemical Precipitation; D004247:DNA / Q000737:chemistry* / Q000235:genetics* / Q000302:isolation & purification; D005826:Genetics, Medical; D006801:Humans; D015336:Molecular Probe Techniques; D015345:Oligonucleotide Probes; D010761:Phosphorus Radioisotopes; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D012313:RNA / Q000737:chemistry / Q000235:genetics
D015345:Oligonucleotide Probes; D010761:Phosphorus Radioisotopes; D012313:RNA; D004247:DNA; D011116:Polynucleotide 5'-Hydroxyl-Kinase