title: Solid-phase synthesis of 2'-deoxy-2'-fluoro- beta-D-oligoarabinonucleotides (2'F-ANA) and their phosphorothioate derivatives.
abstract: This unit describes the chemical synthesis of 2'-deoxy-2'-fluoro-b-D-oligoarabinonucleotides (2'F-ANA), both with phosphodiester and phosphorothioate linkages. The protocols described herein include araF phosphoramidite preparation, assembly on DNA synthesizers, and final deprotection and purification of oligonucleotides.
authors.affiliation: McGill University, Montreal, Canada.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Viazovkina
authors.forename: Maria M
authors.initials: MM
authors.lastname: Mangos
authors.forename: Mohamed I
authors.initials: MI
authors.lastname: Elzagheid
authors.forename: Masad J
authors.initials: MJ
authors.lastname: Damha
mesh_terms: D001088:Arabinonucleotides / Q000138:chemical synthesis*; D001089:Arabinose / Q000031:analogs & derivatives; D009841:Oligonucleotides / Q000138:chemical synthesis*; D054735:Phosphorothioate Oligonucleotides / Q000138:chemical synthesis*
chemical_list: C118537:2'-deoxy-2'-fluoroarabinose; D001088:Arabinonucleotides; D009841:Oligonucleotides; D054735:Phosphorothioate Oligonucleotides; D001089:Arabinose