The protective effect of bergamot oil extract on lecitine-like oxyLDL receptor-1 expression in balloon injury-related neointima formation.
Lectin-like oxyLDL receptor-1 (LOX-1) has recently been suggested to be involved in smooth muscle cell (SMC) proliferation and neointima formation in injured blood vessels. This study evaluates the effect of the nonvolatile fraction (NVF), the antioxidant component of bergamot essential oil (BEO), on LOX-1 expression and free radical generation in a model of rat angioplasty. Common carotid arteries injured by balloon angioplasty were removed after 14 days for histopathological, biochemical, and immunohistochemical studies. Balloon injury led to a significant restenosis with SMC proliferation and neointima formation, accompanied by increased expression of LOX-1 receptor, malondialdehyde and superoxide formation, and nitrotyrosine staining. Pretreatment of rats with BEO-NVF reduced the neointima proliferation together with free radical formation and LOX-1 expression in a dose-dependent manner. These results suggest that natural antioxidants may be relevant in the treatment of vascular disorders in which proliferation of SMCs and oxyLDL-related endothelial cell dysfunction are involved.
Mollace
Vincenzo
V
University "Magna Graecia" of Catanzaro, Italy. mollace@libero.it
Ragusa
Salvatore
S
Sacco
Iolanda
I
Muscoli
Carolina
C
Sculco
Francesca
F
Visalli
Valeria
V
Palma
Ernesto
E
Muscoli
Saverio
S
Mondello
Luigi
L
Dugo
Paola
P
Rotiroti
Domenicantonio
D
Romeo
Francesco
F
D000800:Angioplasty, Balloon / Q000009:adverse effects; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D016893:Carotid Stenosis / Q000209:etiology / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D051127:Scavenger Receptors, Class E / Q000187:drug effects* / Q000235:genetics; D013481:Superoxides / Q000378:metabolism; D017539:Tunica Intima / Q000473:pathology
D000975:Antioxidants; D005609:Free Radicals; C495136:OLR1 protein, rat; D010938:Plant Oils; D051127:Scavenger Receptors, Class E; D013481:Superoxides; C068336:bergamot oil