title: Induction of apoptosis in yeast by L-amino acid oxidase from the Malayan pit viper Calloselasma rhodostoma.
abstract: Here we report for the first time that L-amino acid oxidase (LAAO), a major component of snake venom, induces apoptosis in yeast. The causative agent for induction of apoptosis has been shown to be hydrogen peroxide, produced by the enzymatic activity of LAAO. However, the addition of catalase, a specific hydrogen peroxide scavenger, does not prevent cell demise completely. Intriguingly, depletion of leucine from the medium by LAAO and the interaction of LAAO with yeast cells are shown to be the major factors responsible for cell demise in the presence of catalase.
authors.affiliation: Technische Universität Graz, Institut für Biochemie, Graz, Austria.
authors.forename: Sudharsana Rao
authors.initials: SR
authors.lastname: Ande
authors.forename: Heike
authors.initials: H
authors.lastname: Fussi
authors.forename: Heide
authors.initials: H
authors.lastname: Knauer
authors.forename: Michael
authors.initials: M
authors.lastname: Murkovic
authors.forename: Sandro
authors.initials: S
authors.lastname: Ghisla
authors.forename: Kai-Uwe
authors.initials: KU
authors.lastname: Fröhlich
authors.forename: Peter
authors.initials: P
authors.lastname: Macheroux
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D015169:Colony Count, Microbial; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020287:In Situ Nick-End Labeling; D050830:L-Amino Acid Oxidase / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism*; D008296:Malaysia; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014757:Viper Venoms / Q000737:chemistry* / Q000201:enzymology; D017819:Viperidae
chemical_list: D014757:Viper Venoms; D006861:Hydrogen Peroxide; D002374:Catalase; D050830:L-Amino Acid Oxidase; D007930:Leucine