Engineering disulfide cross-links in RNA via air oxidation.
This unit presents protocols for the synthesis of alkylthiol-modified ribonucleosides, their incorporation into synthetic RNA, and the formation of intramolecular disulfide bonds in RNA by air oxidation. The disulfide bonds can be formed in quantitative yields between thiols positioned in close proximity by virtue of either the secondary or tertiary structure of the RNA. Disulfide cross-links are useful tools to probe solution structures of RNA, to monitor dynamic motion, to stabilize folded RNAs, and to study the process of tertiary structure folding.
Maglott
E J
EJ
University of Michigan, Ann Arbor, USA.
Glick
G D
GD
D000388:Air; D001671:Biochemistry / Q000379:methods*; D003374:Coumarins / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004220:Disulfides / Q000138:chemical synthesis* / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry; D010084:Oxidation-Reduction; D016062:Porosity; D012313:RNA / Q000737:chemistry* / Q000302:isolation & purification; D013438:Sulfhydryl Compounds / Q000737:chemistry; D014529:Uridine / Q000737:chemistry
D003374:Coumarins; D003432:Cross-Linking Reagents; D004220:Disulfides; D009705:Nucleosides; D013438:Sulfhydryl Compounds; D012313:RNA; C030123:coumarin; D014529:Uridine