Structural studies of the O-antigens of Yersinia pseudotuberculosis O:2a and mutants thereof with impaired 6-deoxy-D-manno-heptose biosynthesis pathway.
The full structure of the long- and short-chain O-antigen of Yersinia pseudotuberculosis O:2a containing two uncommon deoxy sugars, abequose and 6-deoxy-d-manno-heptose (6dmanHep), was established, for the first time, by sugar analysis, NMR spectroscopy, and high-resolution ESIMS. Similar structural studies were also performed on two O:2a mutants with single disruption of 6dmanHep synthesis pathway genes each, which synthesize modified long-chain (dmhA mutant) and short-chain (both dmhA and dmhB mutants) O-antigens with 6dmanHep replaced by its putative biosynthetic precursor, D-glycero-D-manno-heptose.
Kondakova
Anna N
AN
ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. annakond@gmail.com
Ho
Nathan
N
Bystrova
Olga V
OV
Shashkov
Alexander S
AS
Lindner
Buko
B
Creuzenet
Carole
C
Knirel
Yuriy A
YA
D002240:Carbohydrate Sequence; D006539:Heptoses / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D008070:Lipopolysaccharides / Q000302:isolation & purification / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D009154:Mutation; D019081:O Antigens / Q000737:chemistry* / Q000235:genetics; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015011:Yersinia pseudotuberculosis / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D006539:Heptoses; D008070:Lipopolysaccharides; D019081:O Antigens; C413547:mannoheptose; C026312:glycero-manno-heptose