title: Capillary electrophoresis of DNA.
abstract: Capillary electrophoresis (CE) is an alternative to conventional slab gel electrophoresis for the separation of DNA fragments. CE offers a number of advantages over slab gel separations in terms of speed, resolution, sensitivity, and data handling. Separation times are generally only a few minutes and the DNA is detected either by UV absorption or by fluorescent labeling. The quantity of DNA required for separation is in the nanogram range. Single-base resolution can be obtained on fragments up to several hundred base pairs. In the presence of appropriate standards, fragments can be accurately sized based on relative electrophoretic mobility. A protocol for the analysis of synthetic oligonucleotides in a flowable matrix is described in this unit.
authors.affiliation: Stanford University, Stanford, California, USA.
authors.forename: Alan
authors.initials: A
authors.lastname: Smith
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Nelson
mesh_terms: D001483:Base Sequence; D019411:Clinical Laboratory Techniques / Q000295:instrumentation; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D008954:Models, Biological; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000032:analysis / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D009841:Oligonucleotides; D004247:DNA