Reovirus preferentially infects the basolateral surface and is released from the apical surface of polarized human respiratory epithelial cells.
Mammalian reoviruses infect respiratory and gastrointestinal epithelia and cause disease in neonates. Junctional adhesion molecule-A (JAM-A) is a serotype-independent receptor for reovirus. JAM-A localizes to tight junctions and contributes to paracellular permeability in polarized epithelia. To investigate the mechanisms of reovirus infection of polarized epithelial cells, we assessed reovirus replication, release, and spread after apical and basolateral adsorption to primary human airway epithelial cultures. Reovirus infection of human airway epithelia was more efficient after adsorption to the basolateral surface than after adsorption to the apical surface, and it was dependent on JAM-A. Reovirus binding to carbohydrate coreceptor sialic acid inhibited apical infection, which was partially ameliorated by treatment of the cultures with neuraminidase. Despite the preference for basolateral infection, reovirus was released from the apical surface of respiratory epithelia and did not disrupt tight junctions. These results establish the existence of an infectious circuit for reovirus in polarized human respiratory epithelial cells.
Excoffon
Katherine J D A
KJ
Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA.
Guglielmi
Kristen M
KM
Wetzel
J Denise
JD
Gansemer
Nicholas D
ND
Campbell
Jacquelyn A
JA
Dermody
Terence S
TS
Zabner
Joseph
J
D000818:Animals; D015815:Cell Adhesion Molecules / Q000378:metabolism; D016764:Cell Polarity / Q000502:physiology; D017097:Electric Impedance; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D007150:Immunohistochemistry; D007739:L Cells; D051379:Mice; D018613:Microscopy, Confocal; D009439:Neuraminidase / Q000494:pharmacology; D030102:Orthoreovirus, Mammalian / Q000378:metabolism / Q000472:pathogenicity*; D016865:Reassortant Viruses / Q000472:pathogenicity; D011956:Receptors, Cell Surface; D012088:Reoviridae Infections / Q000821:virology*; D020545:Respiratory Mucosa / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D012141:Respiratory Tract Infections / Q000821:virology*; D019108:Tight Junctions / Q000378:metabolism / Q000821:virology; D017201:Virus Shedding
D015815:Cell Adhesion Molecules; C407243:F11R protein, human; D007136:Immunoglobulins; D011956:Receptors, Cell Surface; D009439:Neuraminidase