[Effects of 3 genosides on bio-thermodynamic expression of splenic lymphocyte in mice].
OBJECTIVE
To establish a new method for screening active ingredients of Chinese herbs by determining different bio-thermodynamic effects of 3 genosides on splenic lymphocyte of mice.


METHODS
Using a thermal bioactivity monitoring system, the maximum heat output (mHO), average metabolic heat (MH) and constant of decrease rate (DR) of lymphocyte were determined based on the growth metabolic power-time curve, and the outcomes were verified by MIT.


RESULTS
The mHO and MH increased and the DR decreased after lymphocytes being exposed to the 3 genosides in different concentrations, arranged upon their potency as genoside Rg3 > genoside Rg2 > genoside Rg1 (merely insignificant effect). MTT showed the same results.


CONCLUSIONS
Heat activity monitoring system could precisely display the different bio-thermal dynamic effects of 3 genosides on splenic lymphocyte.
Cao
Jun-Ling
JL
Pharmacy Department, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine, Beijing.
Li
Zu-Lun
ZL
Xiao
Xiao-He
XH
D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004734:Energy Metabolism / Q000187:drug effects*; D036145:Ginsenosides / Q000494:pharmacology*; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D013154:Spleen / Q000166:cytology; D013816:Thermodynamics
D036145:Ginsenosides; C026474:ginsenoside Rg2; C035054:ginsenoside Rg1