Influences of interferon-alpha on expression of Th cytokines and CCR7 in dendritic cells from patients with chronic myeloid leukemia in vitro.
This study was aimed to investigate the influences of interferonalpha (IFN-alpha) on expressions of CCR7, interleukin10 (IL-10) and IL-12p70 in dendritic cells (DCs) from patients with chronic myeloid leukemia (CML). In addition to stem cell factor (SCF), granulocyte-macrophage colony stimulating factor (GM-CSF), tumor necrosis factor-alpha (TNF-alpha) and IL-4, IFN-alpha was added to the serum-free medium of DCs. After culture for 10-14 days, phenotypes and function of CML-DCs were evaluated respectively by flow cytometry and methyl thiazolyl tetrazolium (MTT) assay. Chromosome of DCs was analyzed by displaying G banding assay. The concentrations of IL-10 and IL-12P70 in supernatants were evaluated by enzyme-linked immunosorbent assay (ELISA). The results showed that the expressions of CD40, CD83, CD86 and CCR7 and the OD value in allogeneic mixed-lymphocyte reaction (MLR) in group with IFN-alpha (300 U/ml) were twice as high as those in group without IFN-alpha. The percentage of Ph1 positive cells and concentrations of IL-10 and IL-12 P70 were reduced in group with IFN-alpha. It is concluded that the defective phenotypes and functions of CML-DCs can be recruited partly by IFN-alpha. The mechanism may lie in the facts that expression of CCR7 and co-stimulatory molecules is promoted and the inhibitory effect of IL-10 on CML-DCs is relieved partly through the regulation of IFN-alpha.
Zhao
Wen-Li
WL
Department of Hematology, Children Hospital, Suzhou University, Suhou 215003, Jiangsu Province, China. zhaowenli69@yahoo.com.cn
Chai
Yi-Huan
YH
He
Hai-Long
HL
Wei
Xu-Cang
XC
Wang
Tong
T
Xing
Pei-Ni
PN
Li
Mei-Sheng
MS
D002478:Cells, Cultured; D003713:Dendritic Cells / Q000166:cytology*; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D016753:Interleukin-10 / Q000235:genetics / Q000378:metabolism*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000235:genetics / Q000276:immunology*; D010677:Philadelphia Chromosome / Q000187:drug effects; D054400:Receptors, CCR7 / Q000235:genetics / Q000378:metabolism*
C515370:CCR7 protein, human; D016898:Interferon-alpha; D054400:Receptors, CCR7; D016753:Interleukin-10