title: The protective effect of bergamot oil extract on lecitine-like oxyLDL receptor-1 expression in balloon injury-related neointima formation.
abstract: Lectin-like oxyLDL receptor-1 (LOX-1) has recently been suggested to be involved in smooth muscle cell (SMC) proliferation and neointima formation in injured blood vessels. This study evaluates the effect of the nonvolatile fraction (NVF), the antioxidant component of bergamot essential oil (BEO), on LOX-1 expression and free radical generation in a model of rat angioplasty. Common carotid arteries injured by balloon angioplasty were removed after 14 days for histopathological, biochemical, and immunohistochemical studies. Balloon injury led to a significant restenosis with SMC proliferation and neointima formation, accompanied by increased expression of LOX-1 receptor, malondialdehyde and superoxide formation, and nitrotyrosine staining. Pretreatment of rats with BEO-NVF reduced the neointima proliferation together with free radical formation and LOX-1 expression in a dose-dependent manner. These results suggest that natural antioxidants may be relevant in the treatment of vascular disorders in which proliferation of SMCs and oxyLDL-related endothelial cell dysfunction are involved.
authors.affiliation: University "Magna Graecia" of Catanzaro, Italy. mollace@libero.it
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Mollace
authors.forename: Salvatore
authors.initials: S
authors.lastname: Ragusa
authors.forename: Iolanda
authors.initials: I
authors.lastname: Sacco
authors.forename: Carolina
authors.initials: C
authors.lastname: Muscoli
authors.forename: Francesca
authors.initials: F
authors.lastname: Sculco
authors.forename: Valeria
authors.initials: V
authors.lastname: Visalli
authors.forename: Ernesto
authors.initials: E
authors.lastname: Palma
authors.forename: Saverio
authors.initials: S
authors.lastname: Muscoli
authors.forename: Luigi
authors.initials: L
authors.lastname: Mondello
authors.forename: Paola
authors.initials: P
authors.lastname: Dugo
authors.forename: Domenicantonio
authors.initials: D
authors.lastname: Rotiroti
authors.forename: Francesco
authors.initials: F
authors.lastname: Romeo
mesh_terms: D000800:Angioplasty, Balloon / Q000009:adverse effects; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000494:pharmacology*; D016893:Carotid Stenosis / Q000209:etiology / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005609:Free Radicals / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010938:Plant Oils / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D051127:Scavenger Receptors, Class E / Q000187:drug effects* / Q000235:genetics; D013481:Superoxides / Q000378:metabolism; D017539:Tunica Intima / Q000473:pathology
chemical_list: D000975:Antioxidants; D005609:Free Radicals; C495136:OLR1 protein, rat; D010938:Plant Oils; D051127:Scavenger Receptors, Class E; D013481:Superoxides; C068336:bergamot oil