title: The Arabidopsis thaliana aquaglyceroporin AtNIP7;1 is a pathway for arsenite uptake.
abstract: We studied the effect of loss of function in the NIP subfamily II in Arabidopsis thaliana to assess their potential role(s) in arsenite (AsIII) uptake. Loss of function in AtNIP7;1 led to increased plant tolerance to AsIII and reduced total As in planta. AtNIP7;1 expression in various yeast backgrounds increased AsIII sensitivity. In the acr3Delta yeast genotype, AtNIP7;1 caused a moderate increase in AsV tolerance. Short-term As uptake in fsp1Delta expressing AtNIP7;1 was significantly larger than that in the empty vector control. The data suggest that AtNIP7;1 can mediate AsIII transport and contributes to AsIII uptake in plants.
authors.affiliation: Biology Department Area 9, University of York, York YO10 5DD, United Kingdom.
authors.forename: Stanislav V
authors.initials: SV
authors.lastname: Isayenkov
authors.forename: Frans J M
authors.initials: FJ
authors.lastname: Maathuis
mesh_terms: D020346:Aquaporins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D018053:Arsenites / Q000378:metabolism* / Q000633:toxicity; D009154:Mutation
chemical_list: D020346:Aquaporins; D029681:Arabidopsis Proteins; D018053:Arsenites; C530212:NIP7;1 protein, Arabidopsis; C015001:arsenite