Single cell electroporation method for axon tracing in cultured slices.
Axon tracing is an essential method to reveal neuronal connection patterns and characteristics of growing axons during development. Here we introduce an electroporation-based gene transfer technique with a fluorescent protein (FP), which enables us to observe a small number of axons. The result also demonstrates that dynamics of axon behavior can be followed for more than a week. Thus, such labeling with FP is a powerful tool for axon tracing.
Uesaka
Naofumi
N
Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
Nishiwaki
Maki
M
Yamamoto
Nobuhiko
N
D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000196:embryology*; D003712:Dendrites / Q000378:metabolism; D015509:Developmental Biology / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins