Optimization of oligosaccharide synthesis from cellobiose by dextransucrase.
There is a growing market for oligosaccharides as sweeteners, prebiotics, anticariogenic compounds, and immunostimulating agents in both food and pharmaceutical industries. Interest in novel carbohydrate-based products has grown because of their reduced toxicity and low immune response. Cellobiose is potentially valuable as a nondigestible sugar. The reaction of cellobiose, as an acceptor with a sucrose as a donor, catalyzed by a dextransucrase from Leuconostoc mesenteroides B-512FMCM, produced a series of cellobio-oligosaccharides. This production system was optimized using a Box-Behnken experimental design for 289 mM of sucrose and 250 mM of cellobiose and 54 U of the enzyme at pH 5.2 and 30 degrees C, to produce maximum yields of oligosaccharide.
Kim
Misook
M
Department of Food Science, Louisiana State University, Baton Rouge, LA, 70803, USA.
Day
Donal F
DF
D002475:Cellobiose / Q000737:chemistry*; D020650:Combinatorial Chemistry Techniques / Q000379:methods*; D003198:Computer Simulation; D005964:Glucosyltransferases / Q000737:chemistry*; D007934:Leuconostoc / Q000201:enzymology*; D008956:Models, Chemical; D009844:Oligosaccharides / Q000138:chemical synthesis*; D011786:Quality Control
D009844:Oligosaccharides; D002475:Cellobiose; D005964:Glucosyltransferases; C020025:dextransucrase