Measurement of EPF for detection of cow pregnancy using rosette inhibition test.
Early embryonic death of calves due to sub-fertility in cows is of great economic concern to dairy industry. Early pregnancy factor (EPF) is a secretory protein with pregnancy associated immunosuppressive properties. Rosette inhibition test (RIT) was used to detect EPF in inseminated dairy cows. Blood samples were collected at two intervals, 1-3 and 5-7 days after insemination from 23 inseminated and 18 non-inseminated control cows for RIT and pregnancy diagnosis performed between 42 and 45 days on palpation. The study indicates that RIT (P<0.05) has the potential to distinguish pregnant from non-pregnant dairy cows in the first week of pregnancy.
Ghaffari Laleh
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V
Young Researchers Club of Tabriz, Islamic Azad University of Tabriz, Tabriz, East Azerbaijan, Iran. dr-vahidghaffaridvm@hotmail.com
Ghaffari Laleh
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R
Pirany
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N
Moghadaszadeh Ahrabi
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M
D000818:Animals; D002417:Cattle / Q000502:physiology*; D018835:Chaperonin 10 / Q000097:blood*; D005260:Female; D008214:Lymphocytes / Q000378:metabolism; D011247:Pregnancy; D011257:Pregnancy Proteins / Q000097:blood*; D011259:Pregnancy Tests, Immunologic / Q000662:veterinary*; D011270:Pregnancy, Animal / Q000097:blood*; D012397:Rosette Formation / Q000662:veterinary*; D013491:Suppressor Factors, Immunologic / Q000097:blood*
D018835:Chaperonin 10; D011257:Pregnancy Proteins; D013491:Suppressor Factors, Immunologic; C025124:early pregnancy factor