Enantiomer-specific accumulation of hexabromocyclododecanes in eggs of predatory birds.
The diastereomeric composition of hexabromocyclododecane (HBCD) in eggs of peregrine falcon (Falco peregrinus), white-tailed sea eagle (Haliaeetus albicilla), guillemot (Uria aalge) and common tern (Sterna hirundo) as well as the guillemots' main prey, herring (Clupea harengus) was determined by reversed-phase chromatography with triple-stage quadrupole mass spectrometric detection (LC-MS-MS). alpha-HBCD was the predominant diastereomer in all bird species, while in herring, gamma-HBCD made a substantial contribution. Two, as yet unidentified diastereomers, were detected in common tern egg. The alpha-, beta- and gamma-HBCD (+) and (-) enantiomers were separated using a chiral stationary phase. The enantiomer fractions for alpha-HBCD differed substantially between different bird species as well as between guillemot and its prey, herring. Total HBCD levels determined by LC-MS-MS were comparable to those previously obtained by GC-MS.
Janák
K
K
Division of Environmental Medicine, Norwegian Institute of Public Health, P.O. Box 4404, Nydalen, NO-0403 Oslo, Norway. karel.janak@fhi.no
Sellström
U
U
Johansson
A-K
AK
Becher
G
G
de Wit
C A
CA
Lindberg
P
P
Helander
B
B
D000818:Animals; D001717:Birds / Q000033:anatomy & histology / Q000378:metabolism*; D002853:Chromatography, Liquid; D005247:Feeding Behavior; D006842:Hydrocarbons, Brominated / Q000737:chemistry* / Q000378:metabolism*; D010063:Ovum / Q000378:metabolism*; D011235:Predatory Behavior; D012680:Sensitivity and Specificity; D013237:Stereoisomerism; D053719:Tandem Mass Spectrometry
D006842:Hydrocarbons, Brominated; C089796:hexabromocyclododecane