title: Attachment of reporter and conjugate groups to the 3' termini of oligonucleotides.
abstract: Conjugation of oligonucleotides at the 3 terminus is less common because this site is used for covalent linkage to solid-phase oligonucleotide synthesis supports. However, 3-oligonucleotide conjugates have several valuable physicochemical properties, including their ability to stabilize nucleic acid hybridization complexes and to retard the activity of exonucleases. This unit discusses methods for preparing oligonucleotides conjugated at the 3 terminus.
authors.affiliation: Colorado State University, Fort Collins, Colorado, USA.
authors.forename: M M
authors.initials: MM
authors.lastname: Greenberg
mesh_terms: D000586:Amination; D000595:Amino Acid Sequence; D001671:Biochemistry / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D002784:Cholesterol / Q000737:chemistry; D008969:Molecular Sequence Data; D009841:Oligonucleotides / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D010455:Peptides; D011108:Polymers; D013438:Sulfhydryl Compounds; C434331:phosphoramidite; D011092:Polyethylene Glycols; D001710:Biotin; D002784:Cholesterol