The molecular epidemiology of HIV-1 envelope diversity during HIV-1 subtype C vertical transmission in Malawian mother-infant pairs.
OBJECTIVE
To study the relationship between HIV-1 subtype C genetic diversity and mother-to-child transmission and to determine if transmission of HIV-1 V1/V2 env variants occurs stochastically.


METHODS
Case-case-control study of Malawian mother-infant pairs consisting of 32 nontransmitting women, 25 intrauterine transmitters, and 23 intrapartum transmitters in Blantyre, Malawi.


METHODS
A heteroduplex tracking assay against the highly variable HIV-1 env V1/V2 region was used to characterize the relationship between HIV-1 diversity and mother-to-child transmission. The relative abundance of the maternal env variants was quantified and categorized as transmitted or nontransmitted based on the env variants detected in the infant plasma. The V1/V2 region was sequenced from two mother-infant pairs and a phylogenetic tree was built.


RESULTS
No relationship was found between transmission and overall maternal env diversity. Infants had less diverse HIV-1 populations than their mothers, and intrauterine-infected infants had fewer V1/V2 variants and were more likely to harbor a homogeneous V1/V2 population than infants infected intrapartum. V1/V2 sequences cloned from two mother-infant transmission pairs support multiple env variant transmission when multiple variants are detected, rather than single variant transmission followed by diversification. Almost 50% of the HIV-infected infants contained V1/V2 env variants that were not detected in maternal plasma samples. Finally transmission of env variants was not related to their abundance in maternal blood.


CONCLUSIONS
These data suggest that the predominant mechanism(s) of HIV-1 subtype C mother-to-child transmission differs by the timing of transmission and is unlikely to be explained by a simple stochastic model.
Kwiek
Jesse J
JJ
Department of Epidemiology, Malawi College of Medicine, Blantyre, Malawi.
Russell
Elizabeth S
ES
Dang
Kristen K
KK
Burch
Christina L
CL
Mwapasa
Victor
V
Meshnick
Steven R
SR
Swanstrom
Ronald
R
D000328:Adult; D000595:Amino Acid Sequence; D001345:Autoradiography; D016022:Case-Control Studies; D005260:Female; D005312:Fetal Blood / Q000821:virology; D015752:Genes, env; D014644:Genetic Variation; D015658:HIV Infections / Q000235:genetics* / Q000821:virology; D015497:HIV-1 / Q000235:genetics*; D020180:Heteroduplex Analysis; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical; D008295:Malawi; D008957:Models, Genetic; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D011247:Pregnancy