title: Synthesis and ITC characterization of novel nanoparticles constituted by poly(gamma-benzyl L-glutamate)-beta-cyclodextrin.
abstract: Imparting desired technological characteristics to polymeric nanoparticles requires the development of original polymers. In the present work, the synthesis and characterization of a novel PBLG-derivative, the poly(gamma-benzyl L-glutamate)-beta-cyclodextrin (PBLG-beta-CD-50), have been carried out. Nanoparticles from either PBLG-beta-CD-50 polymer or from mixtures with PBLG have been prepared using a modified nanoprecipitation method. Spherically shaped nanoparticles with diameter in the range of 50-70 nm were obtained, as determined by dynamic laser light scattering and transmission electron microscopy. The presence of a surfactant in the suspension medium had almost no influence on these parameters and was not necessary to the shelf-stability of the suspension. Further, isothermal titration microcalorimetry (ITC) experiments have been used to show unambiguously that about 20% of the cyclodextrins remain functional within the particles. Consequently, this system may be of interest when association of large amounts of hydrophobic drugs to nanoparticles is required.
authors.affiliation: UMR CNRS 8612, Laboratoire de Pharmacotechnie et Biopharmacie, Faculté de Pharmacie, Université Paris-Sud, 5 rue J. B. Clément, 92290 Châtenay-Malabry, France.
authors.forename: Ma Elisa Martínez
authors.initials: ME
authors.lastname: Barbosa
authors.forename: Laurent
authors.initials: L
authors.lastname: Bouteiller
authors.forename: Sandrine
authors.initials: S
authors.lastname: Cammas-Marion
authors.forename: Véronique
authors.initials: V
authors.lastname: Montembault
authors.forename: Laurent
authors.initials: L
authors.lastname: Fontaine
authors.forename: Gilles
authors.initials: G
authors.lastname: Ponchel
mesh_terms: D000547:Amantadine / Q000737:chemistry; D002151:Calorimetry / Q000379:methods*; D011232:Chemical Precipitation; D006358:Hot Temperature; D008968:Molecular Conformation; D053758:Nanoparticles / Q000032:analysis* / Q000737:chemistry* / Q000648:ultrastructure; D020442:Poloxamer / Q000737:chemistry; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D047392:beta-Cyclodextrins; C532265:poly(gamma-benzyl glutamate)-beta-cyclodextrin; D020442:Poloxamer; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D000547:Amantadine