title: Topological remodeling of cultured endothelial cells by characterized cyclic strains.
abstract: Evaluation of mechanical environment on cellular function is a major field of study in cellular engineering. Endothelial cells lining the entire vascular lumen are subjected to pulsatile blood pressure and flow. Mechanical stresses caused by such forces determine function of arteries and their remodeling. Critical values of mechanical stresses contribute to endothelial damage, plaque formation and atherosclerosis. A device to impose cyclic strain on cultured cells inside an incubator was designed and manufactured operating with different load amplitudes, frequencies, numbers of cycles and ratios of extension to relaxation. Endothelial cells cultured on collagen coated silicon scaffolds were subjected to cyclic loading. Effects of mechanical loading on cell morphology were quantified using image processing methods. Results showed change in cell orientation from a randomly oriented before the test up to 80 degrees alignment from load axis after loading. Endothelial cells were elongated with shape index reductions up to 47% after cyclic stretch. By increase of strain amplitude, loading frequency and number of cycles, significant decrease in shape index and significant increase in orientation angle were observed. Change of load waveform similar to arterial pulse pressure waveform resulted in alteration of cell alignment with 9.7% decrease in shape index, and 10.8% increase in orientation angle. Results of cyclic loading tests in a disturbed environment with elevated PH showed lack of remodeling. It was concluded that tensile loading of endothelial cells influences cell morphology and alignment, a mechanism for structural regulation, functional adaptation and remodeling. Disturbed environment results in endothelial dysfunction and injury.
authors.affiliation: Faculty of Biomedical Engineering, Amirkabir University of Technology, 424, Hafez Ave., Tehran, Iran.
authors.forename: Nooshin
authors.initials: N
authors.lastname: Haghighipour
authors.forename: Mohammad
authors.initials: M
authors.lastname: Tafazzoli-Shadpour
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.forename: Samira
authors.initials: S
authors.lastname: Amini
authors.forename: Amir
authors.initials: A
authors.lastname: Amanzadeh
authors.forename: Mohammad Taghi
authors.initials: MT
authors.lastname: Khorasani
mesh_terms: D016764:Cell Polarity; D048430:Cell Shape; D002478:Cells, Cultured; D003584:Cytological Techniques; D042783:Endothelial Cells / Q000166:cytology; D004730:Endothelium, Vascular / Q000166:cytology*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008955:Models, Cardiovascular; D013718:Tensile Strength; D014471:Umbilical Veins / Q000166:cytology