Proteomic profiling and identification in peritoneal fluid of children treated by peritoneal dialysis.
BACKGROUND
Proteomic technologies offer a high-throughput analysis of the expression of proteins in biological samples. The global analysis of the proteins in peritoneal dialysis (PD) fluid will provide a better understanding of the biological processes of the peritoneal membrane.


METHODS
The dialysate of nine paediatric PD patients was collected from peritoneal equilibrium tests with 3.86% glucose. Proteins were separated on a 10% SDS-PAGE gel and in-gel digested with trypsin. Peptide mixtures were analysed using nanoLC-MS/MS and results were searched against the NCBI database.


RESULTS
A total number of 189 proteins were identified in the PD fluid of nine patients, with 88 proteins shared by all patients. These 88 proteins accounted for 47% of the identified proteins and >90% of the total protein content in the analysed samples. Proteins were subdivided into eight different classes according to function.


CONCLUSIONS
This study gives a representative overview of the proteins present in PD fluid. The proteins in PD fluid reflect plasma proteins as well as local peritoneal processes. Potentially interesting proteins are revealed.
Raaijmakers
Renske
R
Department of Paediatric Nephrology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands. r.raaijmakers@cukz.umcn.nl
Pluk
Wendy
W
Schröder
Cornelis H
CH
Gloerich
Jolein
J
Cornelissen
Elisabeth A M
EA
Wessels
Hans J C T
HJ
Willems
Johannes L
JL
Monnens
Leo A H
LA
van den Heuvel
Lambert P W J
LP
D001202:Ascitic Fluid / Q000737:chemistry*; D002648:Child; D002675:Child, Preschool; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010530:Peritoneal Dialysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
D020543:Proteome