[Study on SOCS-3, PPARgamma and ACO mRNA expression of adipose tissues in obese rats with leptin resistance].
OBJECTIVE
To study the difference of socs-3, PPARgamma and ACO mRNA expression in obese rats with leptin resistance induced by high-fat diet.


METHODS
6 Wistar male rats were fed with basic diet as control, and 24 rats were fed with high-fat diet as high-fat diet group. At the end of the 8th week, 8 obese rats were selected by body weight from high-fat diet group as obese group. Serum leptin, socs-3, PPARgamma and ACO mRNA were detected.


RESULTS
In compare with control group, body weight, serum leptin, socs-3 and PPARgamma mRNA expressions of epididymal adipose tissues were significantly increased in obese group, while ACO mRNA were markedly decreased.


CONCLUSIONS
Obese rats induced by high-fat diet showed the suppression of leptin signaling transduction pathway, decreases in fatty acid oxidation and increases in fat synthesis.
Liu
Li
L
School of Public Health, China Medical University, Shenyang 110001, China. liuli@mail.cmu.edu.cn
Gu
Hailun
H
Zhao
Yue
Y
Yu
Fei
F
D042962:Acyl-CoA Oxidase / Q000235:genetics / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage; D020738:Leptin / Q000097:blood*; D008297:Male; D009765:Obesity / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D054411:Receptors, Leptin / Q000235:genetics; D015398:Signal Transduction; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism*
D004041:Dietary Fats; D020738:Leptin; D047495:PPAR gamma; D012333:RNA, Messenger; D054411:Receptors, Leptin; C491410:Socs3 protein, rat; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins; D042962:Acyl-CoA Oxidase