Electroblotting from polyacrylamide gels.
This unit contains procedures for electrophoretically transferring proteins onto a variety of membranes including polyvinylidene difluoride (PVDF) and nitrocellulose, and derivatized membranes. The choice of membrane type for electrotransfer is dependent on the ultimate application for the blot membrane. An alternate protocol is provided for electroblotting in semidry systems. This unit also describes procedures for eluting proteins from membranes using detergents or acidic extraction with organic solvents.
Ursitti
J A
JA
The Wistar Institute, Philadelphia, Pennsylvania, USA.
Mozdzanowski
J
J
Speicher
D W
DW
D000180:Acrylic Resins; D015153:Blotting, Western / Q000379:methods*; D003101:Collodion; D003902:Detergents; D004586:Electrophoresis / Q000379:methods*; D008567:Membranes, Artificial; D009930:Organic Chemicals; D011145:Polyvinyls; D011506:Proteins / Q000737:chemistry; D020539:Sequence Analysis, Protein; D012997:Solvents; D005465:Fluorocarbon Polymers
D000180:Acrylic Resins; D003101:Collodion; D003902:Detergents; D008567:Membranes, Artificial; D009930:Organic Chemicals; D011145:Polyvinyls; D011506:Proteins; D012997:Solvents; C016680:polyacrylamide gels; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers