Probing RNA structure by lead cleavage.
Lead cleavage causes a transesterification reaction that breaks the 5',3'-phosphodiester backbone of RNA, leaving a 2',3'-cyclic phosphate and a 5'-hydroxyl. Since the efficiency of the reaction at the 2'-hydroxyl is related to steric and chemical constraints on particular 2'-hydroxyls embedded in the RNA, this reaction can be used to examine the structure of individual nucleotides within an RNA molecule. It is a sensitive probe of tertiary RNA structure, provided that Pb(2+)-binding sites are created in the tertiary structure.
Pan
T
T
University of Chicago, Chicago, Illinois, USA.
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002417:Cattle; D005092:Exonucleases / Q000378:metabolism; D006868:Hydrolysis; D007854:Lead / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D009695:Nucleic Acid Renaturation; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation; D012313:RNA / Q000737:chemistry*; D011130:RNA Ligase (ATP) / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism
D010761:Phosphorus Radioisotopes; D014764:Viral Proteins; D007854:Lead; D012313:RNA; D005092:Exonucleases; D011130:RNA Ligase (ATP); C477285:bacteriophage T4 RNA ligase 1