Spontaneous amplification of the actinorhodin gene cluster in Streptomyces coelicolor involving native insertion sequence IS466.
We observed a spontaneous amplification of the Streptomyces coelicolor chromosome, including genes encoding biosynthetic enzymes of the antibiotic actinorhodin. A new junction of two tandem segments has, inserted within it, a third copy of a transposable element existing in two places elsewhere in the chromosome, suggesting its involvement in the amplification mechanism.
Widenbrant
E M
EM
Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA. ewidenbrant@stanfordalumni.org
Tsai
Hsiu-Hui
HH
Chen
Carton W
CW
Kao
C M
CM
D000880:Anthraquinones / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005784:Gene Amplification; D008957:Models, Genetic; D005810:Multigene Family / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D000095622:Benzoisochromanequinones
D000880:Anthraquinones; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; C013390:actinorhodin; D000095622:Benzoisochromanequinones