title: Biphenyl dioxygenase from an arctic isolate is not cold adapted.
abstract: Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDO(Cam-1)) was purified and found to have an apparent k(cat) for biphenyl of 1.1 +/- 0.1 s(-1) (mean +/- standard deviation) at 4 degrees C. In contrast, BPDO(LB400) from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57 degrees C, the half-life of the BPDO(Cam-1) oxygenase was less than half that of the BPDO(LB400) oxygenase. Nevertheless, BPDO(Cam-1) appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase.
authors.affiliation: Department of Microbiology and Immunology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada.
authors.forename: Emma R
authors.initials: ER
authors.lastname: Master
authors.forename: Nathalie Y R
authors.initials: NY
authors.lastname: Agar
authors.forename: Leticia
authors.initials: L
authors.lastname: Gómez-Gil
authors.forename: Justin B
authors.initials: JB
authors.lastname: Powlowski
authors.forename: William W
authors.initials: WW
authors.lastname: Mohn
authors.forename: Lindsay D
authors.initials: LD
authors.lastname: Eltis
mesh_terms: D001713:Biphenyl Compounds / Q000378:metabolism*; D019117:Burkholderia / Q000201:enzymology; D003080:Cold Temperature; D049308:Dioxygenases / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D004783:Environmental Microbiology; D004795:Enzyme Stability; D006358:Hot Temperature; D007700:Kinetics; D011549:Pseudomonas / Q000201:enzymology* / Q000302:isolation & purification*
chemical_list: D001713:Biphenyl Compounds; D049308:Dioxygenases; C010574:biphenyl