Chromosomal radiosensitivity in head and neck cancer patients: evidence for genetic predisposition?
The association between chromosomal radiosensitivity and genetic predisposition to head and neck cancer was investigated in this study. In all, 101 head and neck cancer patients and 75 healthy control individuals were included in the study. The G(2) assay was used to measure chromosomal radiosensitivity. The results demonstrated that head and neck cancer patients had a statistically higher number of radiation-induced chromatid breaks than controls, with mean values of 1.23 and 1.10 breaks per cell, respectively (P<0.001). Using the 90th percentile of the G(2) scores of the healthy individuals as a cutoff value for chromosomal radiosensitivity, 26% of the cancer patients were radiosensitive compared with 9% of the healthy controls (P=0.008). The mean number of radiation-induced chromatid breaks and the proportion of radiosensitive individuals were highest for oral cavity cancer patients (1.26 breaks per cell, 38%) and pharynx cancer patients (1.27 breaks per cell, 35%). The difference between patients and controls was most pronounced in the lower age group (<or=50 years, 1.32 breaks per cell, 38%) and in the non- and light smoking patient group (<or=10 pack-years, 1.28 breaks per cell, 46%). In conclusion, enhanced chromosomal radiosensitivity is a marker of genetic predisposition to head and neck cancer, and the genetic contribution is highest for oral cavity and pharynx cancer patients and for early onset and non- and light smoking patients.
De Ruyck
K
K
Department of Human Anatomy, Embryology, Histology and Medical Physics, Ghent University, Proeftuinstraat 86, Ghent B-9000, Belgium. kim.deruyck@UGent.be
de Gelder
V
V
Van Eijkeren
M
M
Boterberg
T
T
De Neve
W
W
Vral
A
A
Thierens
H
H
D000230:Adenocarcinoma / Q000235:genetics; D000328:Adult; D000367:Age Factors; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics; D002842:Chromatids / Q000528:radiation effects; D002877:Chromosomes, Human / Q000528:radiation effects*; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects; D004273:DNA, Neoplasm; D005260:Female; D016195:G2 Phase / Q000528:radiation effects*; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006258:Head and Neck Neoplasms / Q000209:etiology / Q000235:genetics* / Q000532:radiotherapy; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics; D016017:Odds Ratio; D010610:Pharyngeal Neoplasms / Q000235:genetics; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects*
D004273:DNA, Neoplasm; D005819:Genetic Markers