Comparative proteomics analysis of male and female Persian sturgeon (Acipenser persicus) gonads.
In sturgeon aquaculture, the fish are sexed by an invasive surgical examination of the gonads. Development of a non-invasive procedure for sexing fish based on a molecular method is of special interest. In the present study a proteomics approach has been utilized to analyze a differential protein expression between mature male and female Persian sturgeon (Acipenser persicus) gonads. When comparing protein patterns on the 2-DE gels of the testis and ovary, 48 unique spots were distinguished in testis while only two spots were matchless in ovary. The spots were identified by MALDI-TOF/TOF analysis. The identified proteins are involved in metabolism and energy production, cell structure, transcription and translation, cell defense, signal transduction, transport, cell division, and none were directly linked to a sex-determining gene. The provided proteomics data could be considered as a starting base for subsequent studies focusing on the identification of proteins involved in sex determination and differentiation at different stages of gonadal maturation.
Keyvanshokooh
Saeed
S
Department of Fisheries, Tarbiat Modares University, Mazandaran, Iran.
Kalbassi
Mohammad Reza
MR
Hosseinkhani
Saman
S
Vaziri
Behrouz
B
D000818:Animals; D017756:Aquaculture; D015180:Electrophoresis, Gel, Two-Dimensional / Q000662:veterinary; D005260:Female; D005399:Fishes / Q000378:metabolism*; D008297:Male; D010053:Ovary / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D012732:Sex Determination Analysis / Q000662:veterinary*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000662:veterinary; D013737:Testis / Q000378:metabolism*