[Change of chart genes expression in small intestines of mouse induced by electromagnetic pulse irradiation].
OBJECTIVE
To explore the bio-effects of electromagnetic pulse(EMP) on mouse small intestines induced by means of gene chip.


METHODS
Twelve BALB/c mice were randomly assigned to the normal control group and the EMP group with 6 in each group. The EMP group was irradiated with 200 kV/m, 200 pulses EMP. 18 hours after the irradiation, the mice were sacrificed and their jejunum of small intestines were eviscerated. The fluorescent cDNA probes labeled with Cy3 and Cy5 were prepared from RNA extracted from the intestines of the two groups. Probes of the two groups were then hybridized against cDNA gene chip, the fluorescent signals were scanned with a scanner and the results were analyzed by computer.


RESULTS
Compared with the control, 56 genes in gene expression profile were altered. The expression levels of 37 genes were up-regulated distinctly while 19 genes were down-regulated significantly. Among the 56 genes, 19 were reported with known or inferred functions, 12 up-regulated genes were catenin alpha 1 (alpha-catenin), ly-6 alloantigen(Ly-6E), fructose-6-phosphate transaminase (GF6P), ribosomal protein S17 (rpS17), small proline-rich protein 2A (Sprr2a), glandular kallikrein27 (GK27), lipoxygenase-3, aldo-keto reductase (Akr1c12), GSG1, amylase 2 (Amy2),elastase 2, p6-5 gene and 7 down-regulated genes were junctional adhesion molecule (Jam), protein arginine methyltransferase (Carm1),NNP-1, 2-5 A synthetase L2,Mlark gene, ATP synthase alpha subunit, uncoupling protein-2 (Ucp2) gene; the other 37 were reported with unknown functions.


CONCLUSIONS
EMP irradiation could induce specific expressions of some genes in mouse small intestines and most of these genes were up-regulated ones.
Ren
Dongqing
D
Department of Radiation Medicine, Faculty of military Preventive Medicine, Fourth Military Medical University, Xi'an 710032, China. yrendq@fmmu.edu.cn
Jin
Juan
J
Li
Xiaojuan
X
Zeng
Guiying
G
D000818:Animals; D004574:Electromagnetic Fields; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects; D007421:Intestine, Small / Q000378:metabolism* / Q000528:radiation effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D015854:Up-Regulation / Q000528:radiation effects