title: Adiponectin promotes endothelial progenitor cell number and function.
abstract: Obesity-linked diseases are associated with suppressed endothelial progenitor cell (EPC) function. Adiponectin is an adipose-derived protein that is downregulated in obese and diabetic subjects. Here, we investigated the effects of adiponectin on EPCs. EPC levels did not increase in adiponectin deficient (APN-KO) in response to hindlimb ischemia. Adenovirus-mediated delivery of adiponectin increased EPC levels in both WT and APN-KO mice. Incubation of human peripheral blood mononuclear cells with adiponectin led to an increase of the number of EPCs. Adiponectin induced EPC differentiation into network structures and served as a chemoattractant in EPC migration assays. These data suggest that hypoadiponectinemia may contribute to the depression of EPC levels that are observed in patients with obesity-related cardiovascular disorders.
authors.affiliation: Department of Cardiology, Nagoya University Graduate School of Medicine, Nagoya, Japan. rshibata@med.nagoya-u.ac.jp
authors.forename: Rei
authors.initials: R
authors.lastname: Shibata
authors.forename: Carsten
authors.initials: C
authors.lastname: Skurk
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Ouchi
authors.forename: Gennaro
authors.initials: G
authors.lastname: Galasso
authors.forename: Kazuhisa
authors.initials: K
authors.lastname: Kondo
authors.forename: Taiki
authors.initials: T
authors.lastname: Ohashi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimano
authors.forename: Shinji
authors.initials: S
authors.lastname: Kihara
authors.forename: Toyoaki
authors.initials: T
authors.lastname: Murohara
authors.forename: Kenneth
authors.initials: K
authors.lastname: Walsh
mesh_terms: D000256:Adenoviridae; D052242:Adiponectin / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation / Q000235:genetics; D002465:Cell Movement; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection
chemical_list: D052242:Adiponectin