Bone-like tissue formation on a biomimetic titanium surface in an explant model of osteoconduction.
The clinical use of titanium in dental and orthopedic applications is limited. Over recent years, implant surfaces have undergone numerous modifications to enhance bone integration. In this study, we experimented a bioactive titanium using a simple chemical and moderate heat treatment that led to the formation of a bone-like apatite layer on its surface in simulated body fluids. We used a bone explant model to demonstrate that cells can migrate from the explants and subsequently differentiate to form a mineralized nodular structure. Furthermore, these cells expressed alkaline phosphatase, bone sialoprotein, osteocalcin and the transcription factor, Runx2. Using this model of osteoconduction, we showed that bioactive titanium bonds directly to bone, while pure titanium cannot. These findings show the importance of implant surface composition in promoting osteogenic cell differentiation and subsequent apposition of the bone matrix, allowing strong bonds to form. This model could be particularly beneficial to closely mimic bone formation adjacent to endosseous implants.
Isaac
Juliane
J
INSERM, U, Laboratoire de Biologie Oro-faciale et Pathologie, Paris, France. isaacjuliane@yahoo.fr
Loty
Sabine
S
Hamdan
Ahmad
A
Kokubo
Tadashi
T
Kim
Hyun-Min
HM
Berdal
Ariane
A
Sautier
Jean-Michel
JM
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D040761:Biomimetic Materials / Q000494:pharmacology*; D001861:Bone Regeneration / Q000187:drug effects*; D001842:Bone and Bones / Q000166:cytology / Q000187:drug effects* / Q000201:enzymology / Q000648:ultrastructure; D008954:Models, Biological; D010012:Osteogenesis / Q000187:drug effects*; D051381:Rats; D012886:Skull / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000648:ultrastructure; D013499:Surface Properties / Q000187:drug effects; D014025:Titanium / Q000494:pharmacology*
D014025:Titanium; D000469:Alkaline Phosphatase