Isolation of human B cell populations.
To study the function and biology of human B cells, it is necessary to isolate pure populations. Historically, B cells were enriched by the sequential depletion of monocytes, natural killer cells, and T cells. However, this time-consuming process has been superseded by negative selection methods using antibody cocktails targeted against other cell types or by positive selection using antibodies targeting B cell markers such as CD19. Here we detail three methods of isolating B cells from human blood or mononuclear cells and describe how these techniques can be combined with fluorescent cell sorting for the characterization of specific B cell populations.
Sims
Gary P
GP
National Institutes of Health, Bethesda, Maryland, USA.
Lipsky
Peter E
PE
D018941:Antigens, CD19 / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000502:physiology; D001773:Blood Cells / Q000166:cytology; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods; D008212:Lymphocyte Depletion
D018941:Antigens, CD19