Analysis of oxidative modification of proteins.
Protein oxidation has been implicated in a variety of degenerative diseases as well as in the aging process. This unit describes techniques for the quantification of various protein oxidation products, including protein carbonyls, loss of protein thiol groups, dityrosine and nitrotyrosine, and isoaspartate formation. Such oxidatively modified products may also be used as biomarkers for the assessment of oxidative stress during aging and/or disease.
Yan
L J
LJ
Southern Methodist University, Dallas, Texas, USA.
Sohal
R S
RS
D001671:Biochemistry / Q000379:methods*; D001894:Borohydrides / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D007460:Iodoacetamide / Q000378:metabolism; D026581:Isoaspartic Acid / Q000032:analysis; D013058:Mass Spectrometry; D010084:Oxidation-Reduction; D010659:Phenylhydrazines / Q000378:metabolism; D050050:Protein Carbonylation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D012015:Reference Standards; D013053:Spectrophotometry; D013438:Sulfhydryl Compounds / Q000378:metabolism; D014316:Tritium; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000378:metabolism
D001894:Borohydrides; D026581:Isoaspartic Acid; D010659:Phenylhydrazines; D011506:Proteins; D013438:Sulfhydryl Compounds; D014316:Tritium; C004787:2,4-dinitrophenylhydrazine; C002744:3-nitrotyrosine; D014443:Tyrosine; C025364:sodium borohydride; C007543:dityrosine; D007460:Iodoacetamide