Age-related correlation between antioxidant enzymes and DNA damage with smoking and body mass index.
To understand whether oxidants contribute to the initiation and/or promulgation toward aging, the present study has been undertaken on 220 healthy male volunteers aged 20-80 years selected from the defined electoral area (suburbs of Tirupati, Andhra Pradesh, India) to evaluate the concentrations of free radicals (superoxide anion, hydrogen peroxide), lymphocyte antioxidant enzymes (glutathione S-transferase, superoxide dismutase, catalase), and DNA damage in relation to obesity and smoking (lifestyles). A two fold increase of lymphocyte free radical generation (DNA damage) was observed in older age groups with a reduced antioxidant potential, forming a link between cigarette smoking and oxidative stress represented by an antioxidant imbalance. Body mass index had a positive relationship with oxidative stress, but antioxidant levels did not vary with body mass index. The findings conclude that free radical-mediated oxidative stress and DNA damage accelerate with lifestyle variations under reduced antioxidant potential.
Reddy Thavanati
Parvathi Kumara
PK
Instituto de Genética, Departamento de Biología Molecular y Genomica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, México. tpkreddy@yahoo.com
Kanala
Kodanda Reddy
KR
de Dios
Aurora Escoto
AE
Cantu Garza
Jose Maria
JM
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000235:genetics* / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D015992:Body Mass Index; D002374:Catalase / Q000378:metabolism; D004249:DNA Damage; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D008214:Lymphocytes / Q000201:enzymology; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000503:physiopathology*; D018384:Oxidative Stress / Q000502:physiology; D012907:Smoking / Q000503:physiopathology*; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D002374:Catalase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase