title: Short-term results of penetrating keratoplasty performed with the Femtec femtosecond laser.
abstract: OBJECTIVE
  To evaluate the use of the Femtec femtosecond (fs) laser for penetrating keratoplasty (PK) in the treatment of corneal diseases.
  
  
  METHODS
  Prospective, nonrandomized clinical study.
  
  
  METHODS
  Nine eyes of nine patients underwent surgery for PK. Five had pseudophakic bullous keratopathy, three had Fuchs dystrophy, and one presented in a keratoconus patient. A Femtec (20/10 PerfectVision; GmbH, Heidelberg, Germany) fs laser was used to create penetrating cuts on donor and recipient corneas. All patients were evaluated for uncorrected visual acuity (UCVA), best spectacle-corrected visual acuity (BSCVA), pachymetry, topography, and endothelial cell density (ECD). Scanning electron microscopy (SEM) was performed on corneal tissue after surgery.
  
  
  RESULTS
  All eyes were treated successfully without intraoperative complications. The mean follow-up was 6 +/- 3 months. At the last postoperative examination mean BSCVA was 20/69 and there was a significant improvement (P = .08) in both UCVA and BSCVA. Mean astigmatism was 2.9 +/- 1.2 diopters. Mean ECD was 1194 +/- 465 cells/mm(2) with a mean cell loss after surgery of 49.8% +/- 19.8%. SEM displayed smooth rectilinear cut margins and minor remaining tissue bridges. One patient presented a retinal detachment three months after surgery that was successfully treated and two subjects showed an allograft rejection.
  
  
  CONCLUSIONS
  Use of the Femtec fs laser was effective and safe to perform PK. Short-term visual results and refractive results are analogous to conventional PK or other fs laser-assisted PK studies. Longer-term follow-up of additional cases is necessary to precisely quantify the endothelial cell loss after fs surgery.
authors.affiliation: Department of Ophthalmology, Hopital de la Timone, 264 rue Saint-Pierre, Marseille cedex 05, France. louis.hoffart@ap-hm.fr
authors.forename: Louis
authors.initials: L
authors.lastname: Hoffart
authors.forename: Helene
authors.initials: H
authors.lastname: Proust
authors.forename: Frederic
authors.initials: F
authors.lastname: Matonti
authors.forename: Bernard
authors.initials: B
authors.lastname: Ridings
authors.forename: John
authors.initials: J
authors.lastname: Conrath
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002452:Cell Count; D003315:Cornea / Q000601:surgery* / Q000648:ultrastructure; D003316:Corneal Diseases / Q000601:surgery*; D019781:Corneal Topography; D004728:Endothelium, Corneal / Q000473:pathology; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D015948:Keratoplasty, Penetrating / Q000295:instrumentation / Q000379:methods*; D053844:Lasers, Solid-State / Q000627:therapeutic use*; D008297:Male; D008855:Microscopy, Electron, Scanning; D008875:Middle Aged; D011446:Prospective Studies; D016896:Treatment Outcome; D014792:Visual Acuity / Q000502:physiology