title: Use of electrophilic substitution to form site-specific cross-links in DNA.
abstract: This unit describes the synthesis, purification, and analysis of modified oligodeoxynucleotides containing 4-thio-2'-deoxyuridine, and the use of these modified oligodeoxynucleotides in conjunction with bis-electrophilic tethers to form site-specific cross-links with a complementary DNA target. These modified oligodeoxynucleotides provide a flexible tool for studies directed at biophysical investigation of oligonucleotide structure, as well as investigation of DNA hybridization, stability, and sequence-dependent base nucleophilicity.
authors.affiliation: The Ohio State University, Columbus, Ohio, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Pires
authors.forename: R S
authors.initials: RS
authors.lastname: Coleman
mesh_terms: D001671:Biochemistry / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D010655:Phenylenediamines / Q000737:chemistry
chemical_list: D003432:Cross-Linking Reagents; D010655:Phenylenediamines; D004247:DNA