title: Immobilization of proteins to biacore sensor chips using Staphylococcus aureus sortase A.
abstract: The immobilization of proteins to surfaces is an active area of research due to strong interest in protein-based sensors. Here, we describe a novel method for immobilizing ligand proteins onto Biacore sensor chips using the transpeptidase activity of Staphylococcus aureus sortase A (SrtA). This method provides a robust and gentle approach for the site-directed, covalent coupling of proteins to biosensor chips. Notably, the high specificity of the sortase allows immobilization of proteins from less than pure protein samples allowing short cuts in protein purification protocols.
authors.affiliation: Department of Molecular Medicine & Pathology, School of Medical Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand.
authors.forename: Fiona
authors.initials: F
authors.lastname: Clow
authors.forename: John D
authors.initials: JD
authors.lastname: Fraser
authors.forename: Thomas
authors.initials: T
authors.lastname: Proft
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D020816:Amino Acid Motifs; D019881:Aminoacyltransferases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D015374:Biosensing Techniques / Q000379:methods*; D017242:Complement Factor H / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D006801:Humans; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013211:Staphylococcus aureus / Q000201:enzymology*
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D011506:Proteins; D011993:Recombinant Fusion Proteins; C078635:fibronectin-binding proteins, bacterial; D017242:Complement Factor H; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases