title: Column chromatography.
abstract: Column chromatography is used extensively in nucleic acid chemistry to purify or characterize products from chemical reactions. This appendix focuses on separations using silica gel or alumina. The procedures described include loading the column, assembling the apparatus, separating compounds and collecting fractions, and optimizing chromatography conditions.
authors.affiliation: Purdue University, West Lafayette, Indiana, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Meyers
mesh_terms: D000327:Adsorption; D002845:Chromatography / Q000295:instrumentation* / Q000379:methods*; D009696:Nucleic Acids / Q000302:isolation & purification
chemical_list: D009696:Nucleic Acids