Stage-specific expression of ankyrin and SOCS box protein-4 (Asb-4) during spermatogenesis.
Members of the large family of Asb proteins are ubiquitously expressed in mammalian tissues; however, the roles of individual Asb and their function in the developmental testes have not been reported. In this report, we isolated a murine Asb4 from mouse testis. Northern blot analysis revealed that mAsb-4 was expressed only in testes and produced in a stage-specific manner during spermatogenesis. It was expressed in murine testes beginning in the fourth week after birth and extending into adulthood. Pachytene spermatocytes had the highest level of expression. Interestingly, the human homologue of mAsb-4, ASB-4 (hASB-4) was also expressed in human testis. These results suggest that ASB-4 plays pivotal roles in mammalian testis development and spermatogenesis.
Kim
Soo-Kyoung
SK
Department of Anatomy and Cell Biology and Department of Biomedical Sciences, College of Medicine, Hanyang University, Seoul 133-791, Korea.
Rhim
Si Youn
SY
Lee
Man Ryul
MR
Kim
Jong Soo
JS
Kim
Hyung Jun
HJ
Lee
Dong Ryul
DR
Kim
Kye-Seong
KS
D000595:Amino Acid Sequence; D000818:Animals; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D013091:Spermatogenesis / Q000235:genetics*; D050826:Suppressor of Cytokine Signaling Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
C530435:Asb4 protein, mouse; D012333:RNA, Messenger; D050826:Suppressor of Cytokine Signaling Proteins