title: Simultaneous determination of dopamine, ascorbic acid and uric acid at poly (Evans Blue) modified glassy carbon electrode.
abstract: A sensitive and selective electrochemical method for the determination of dopamine using an Evans Blue polymer film modified on glassy carbon electrode was developed. The Evans blue polymer film modified electrode shows excellent electrocatalytic activity toward the oxidation of dopamine in phosphate buffer solution (pH 4.5). The linear range of 1.0 x 10(-6)-3.0 x 10(-5) M and detection limit of 2.5 x 10(-7) M were observed in pH 4.5 phosphate buffer solutions. The interference studies showed that the modified electrode exhibits excellent selectivity in the presence of large excess of ascorbic acid and uric acid. The separation of the oxidation peak potentials for dopamine-ascorbic acid and dopamine-uric acid were about 182 mV and 180 mV, respectively. The differences are large enough to determine AA, DA and UA individually and simultaneously. This work provides a simple and easy approach to selectively detect dopamine in the presence of ascorbic acid and uric acid in physiological samples.
authors.affiliation: Department of pharmaceutical analysis, Faculty of Pharmacy, Fujian Medical University, Fuzhou, China.
authors.forename: Liqing
authors.initials: L
authors.lastname: Lin
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
authors.forename: Hong
authors.initials: H
authors.lastname: Yao
authors.forename: Yuanzhong
authors.initials: Y
authors.lastname: Chen
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Zheng
authors.forename: Xinhua
authors.initials: X
authors.lastname: Lin
mesh_terms: D001205:Ascorbic Acid / Q000032:analysis*; D002244:Carbon / Q000737:chemistry*; D004298:Dopamine / Q000032:analysis*; D004563:Electrochemistry; D004566:Electrodes; D005070:Evans Blue / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D006851:Hydrochloric Acid; D006863:Hydrogen-Ion Concentration; D015394:Molecular Structure; D010084:Oxidation-Reduction; D014527:Uric Acid / Q000032:analysis*; D014556:Urine / Q000737:chemistry
chemical_list: D014527:Uric Acid; D005070:Evans Blue; D002244:Carbon; D001205:Ascorbic Acid; D006851:Hydrochloric Acid; D004298:Dopamine