Slot-blot analysis of 3-nitrotyrosine-modified brain proteins.
3-Nitrotyrosine (3-NT) is used as a biomarker of nitrosative stress. The formation of 3-NT has been reported in a number of diseases, including Alzheimer's disease, Huntington's disease, Parkinson's disease, amyotrophic lateral sclerosis, and cancer. The nitration of proteins is a reversible process, but it can induce a conformational change and thereby functional alterations of the affected protein. 3-NT measurements in biological samples are usually carried out by methodologies such as immunohistochemistry, high-performance liquid chromatography, gas chromatography, and immunochemical detection. This chapter describes the immunochemical method for the determination of protein-bound 3-NT using slot-blot analysis.
Sultana
Rukhsana
R
Department of Chemistry, University of Kentucky, Lexington, Kentucky, USA.
Butterfield
D Allan
DA
D000818:Animals; D001921:Brain / Q000378:metabolism*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D009419:Nerve Tissue Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry / Q000378:metabolism
D009419:Nerve Tissue Proteins; C002744:3-nitrotyrosine; D014443:Tyrosine