Electrokinetic sorting and collection of fractions for preparative capillary electrophoresis on a chip.
A microfabricated device capable of selecting and collecting multiple components from a mixture separated by capillary electrophoresis (CE) is described. This collection is automated and can be easily controlled by a set of rules defined by an operator, enabling fast and consistent operation. The device consists of an electrokinetically steered fluidic network that can be divided into three sections: a CE part, a fractions distribution region and a set of storage channels. Sample fractions leave the CE channel and are detected in the interfacial region by fluorescence intensity measurements. If an upcoming peak is detected, separation is withheld and the potentials are reconfigured to force the fraction into one of the collection channels, where they become available for further processing or analysis. The sequence of separation and collection is repeated until all the bands of interest are captured. A mixture of three fluorescent dyes (Rhodamine 6G, Rhodamine B and Fluorescein) was used to demonstrate the principle. The components were repeatedly separated by means of CE and pooled in their respective storage channels. In comparison to previous developments, the system presented in this paper offers automatic collection of all fractions in a single run. Furthermore, it is possible to run the system in a repetitive mode for accumulative pooling if more fractionated sample is required.
Zalewski
Dawid R
DR
MESA+ Institute for Nanotechnology, Faculty of Science and Technology, University of Twente, P.O Box 217, 7500AE, Enschede, The Netherlands. d.r.zalewski@utwente.nl
Schlautmann
Stefan
S
Schasfoort
Richard B M
RB
Gardeniers
Han J G E
HJ
D004563:Electrochemistry; D046209:Electrophoresis, Microchip / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019793:Fluorescein / Q000032:analysis; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis*; D007700:Kinetics; D046228:Microarray Analysis / Q000295:instrumentation* / Q000379:methods; D015203:Reproducibility of Results; D012235:Rhodamines / Q000032:analysis; D013997:Time Factors
D005456:Fluorescent Dyes; D012235:Rhodamines; C026188:rhodamine 6G; C029773:rhodamine B; D019793:Fluorescein