The decapeptide CMS001 enhances swimming endurance in mice.
Now peptides achieve distinct advantages over protein in biological application because of its quick and easy absorption, low power, and high activity. Some bioactive peptides had been developed to be used in the management of exercise-related disorders. In this study, we investigated whether the decapeptide CMS001 (Pro-Thr-Thr-Lys-Thr-Tyr-Phe-Pro-His-Phe) isolated from pig spleen had anti-fatigue effects. Male Balb/c mice were administered CMS001 (20 microg/(kgd)(-1) or 5 microg/(kgd)(-1) for 30 d, intraperitoneal injections) and tested in an exhaustive swim time task. In order to examine the mechanisms of CMS001 anti-fatigue effects, we analyzed liver glycogen stores, blood urea nitrogen (BUN) levels, lactic acid levels, ultrastructural integrity, and levels of both a free radical metabolite and an anti-oxidant enzyme. CMS001 treatment prolonged exhaustive swim time, increased liver glycogen levels, reduced BUN levels, and decreased accumulation of lactic acid in the blood, relative to mice injected with only saline. Examination of the ultrastructure of mitochondria and sarcoplasmic reticulum in skeletal and cardiac muscle of CMS001-treated and control mice revealed that CMS001 can reduce the damage to cardiac and skeletal muscle caused by an exhaustive swim challenge, such that the structure of most tissue specimens were normal in the peptide-treated group. Furthermore the free radical analysis after acute exercise indicated that CMS001 treatment decreased malondialdehyde (MDA) and increased superoxide dismutase (SOD) levels. The present findings indicate that the spleen-derived peptide CMS001 has anti-fatigue effects in mice, and further suggest that the mechanism may involve reduction of tissue damaging free radicals in muscle tissues.
Wang
Li
L
Department of Immunology, Tianjin Medical University, Tianjin 300070, China.
Zhang
Hua-Li
HL
Lu
Rong
R
Zhou
Yan-Jiao
YJ
Ma
Rui
R
Lv
Jun-Qiang
JQ
Li
Xiao-Lei
XL
Chen
Li-Juan
LJ
Yao
Zhi
Z
D000818:Animals; D001806:Blood Urea Nitrogen; D019344:Lactic Acid / Q000097:blood; D008112:Liver Glycogen / Q000032:analysis / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000648:ultrastructure; D009206:Myocardium / Q000648:ultrastructure; D009842:Oligopeptides / Q000494:pharmacology*; D010807:Physical Endurance / Q000187:drug effects*; D011897:Random Allocation; D013047:Specific Pathogen-Free Organisms; D013482:Superoxide Dismutase / Q000097:blood; D013550:Swimming; D013997:Time Factors
D008112:Liver Glycogen; D009842:Oligopeptides; C531447:Pro-Thr-Thr-Lys-Thr-Tyr-Phe-Pro-His-Phe; D019344:Lactic Acid; D008315:Malondialdehyde; D013482:Superoxide Dismutase