The effects of mineral trioxide aggregates on cytokine production by mouse pulp tissue.
OBJECTIVE
The objective of this study was to determine the effect of MTA on the expression of cytokines in mouse pulp tissue.


METHODS
Pulp tissue was exposed to MTA, and the expression of CCL5/RANTES, CCL2/MCP1, IL-1alpha, IFN-gamma, TNF-alpha, IL-4, and IL-6 was evaluated by RT-PCR at 10 and 20 days after exposure. Control groups were not exposed to MTA.


RESULTS
We found no detectable expression of CCL2, IL-4, and IL-6 in the tissue from either group, while TNF-alpha was expressed at high levels 20 days after exposure (P < .05). CCL5 and IL-1alpha mRNA expression was lower in the MTA-treated group 10 days after treatment (P < .05). At 20 days after the surgical procedure, IFN-gamma mRNA expression was also lower in the MTA-treated group (P < .05).


CONCLUSIONS
These findings suggest that MTA down-regulates the inflammatory cytokines CCL5, IL-1alpha and IFN-gamma and may have an anti-inflammatory effect.
Barbosa Silva
Marcelo José
MJ
Department of Biochemistry and Immunology, Institute of Biological Science, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Vieira
Leda Quercia
LQ
Sobrinho
Antônio Paulino Ribeiro
AP
D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D017610:Calcium Compounds / Q000494:pharmacology*; D018946:Chemokine CCL5 / Q000096:biosynthesis; D016207:Cytokines / Q000096:biosynthesis*; D003782:Dental Pulp / Q000187:drug effects* / Q000378:metabolism; D003785:Dental Pulp Capping / Q000379:methods; D015536:Down-Regulation; D004338:Drug Combinations; D015870:Gene Expression; D018836:Inflammation Mediators / Q000378:metabolism; D007371:Interferon-gamma / Q000096:biosynthesis; D007375:Interleukin-1 / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D010087:Oxides / Q000494:pharmacology*; D011672:Pulpotomy / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D017607:Aluminum Compounds; D017610:Calcium Compounds; D018946:Chemokine CCL5; D016207:Cytokines; D004338:Drug Combinations; D018836:Inflammation Mediators; D007375:Interleukin-1; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; D014409:Tumor Necrosis Factor-alpha; C086631:mineral trioxide aggregate; D007371:Interferon-gamma