Universal 2-(4-nitrophenyl)ethyl and 2-(4-nitrophenyl)ethoxycarbonyl protecting groups for nucleosides and nucleotides.
A universal blocking group strategy for nucleobases is described, using the 2-(4-nitrophenyl)ethyl (NPE) group for O4-T-, O4-U-, O6-dG-, and O6-G-protection as well as the 2-(4-nitrophenyl)ethoxycarbonyl (NPEOC) group for amino protection in dC, C, dA, A, dG, and G. Conversion into the corresponding 5'-O-dimethoxytrityl derivatives and subsequent phosphitylation to form the fully protected 3'-O-(2-cyanoethyl-N,N-diisopropylphosphoramidites) and 3'-O-(2-(4-nitrophenyl)ethyl-N,N-diisopropylphosphoramidites) produces a new class of interesting building blocks for oligonucleotide synthesis.
Pfleiderer
Wolfgang
W
Konstanz University, Konstanz, Germany.
D009682:Magnetic Resonance Spectroscopy; D009578:Nitrobenzenes / Q000737:chemistry*; D009705:Nucleosides / Q000737:chemistry*; D009711:Nucleotides / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D009578:Nitrobenzenes; D009705:Nucleosides; D009711:Nucleotides