Use of chemical modification to elucidate RNA folding pathways.
As discussed in this overview, chemical modification is sensitive to the accessibility of a nucleotide to the solvent, and many nucleotides become less accessible as an RNA folds into its structured form. Chemical modification reagents are therefore suitable for following RNA folding, and can be used to study the kinetics of structure formation on time scales ranging from minutes to hours.
Mathews
David H
DH
University of Rochester, Rochester, New York, USA.
Turner
Douglas H
DH
D007202:Indicators and Reagents; D007700:Kinetics; D008722:Methods; D009690:Nucleic Acid Conformation; D012313:RNA / Q000737:chemistry*; D012997:Solvents
D007202:Indicators and Reagents; D012997:Solvents; D012313:RNA