title: Infection rate and virus-induced cytokine secretion in experimental rhinovirus infection in mucosal organ culture: comparison between specimens from patients with chronic rhinosinusitis with nasal polyps and those from normal subjects.
abstract: OBJECTIVE
  To investigate the difference in susceptibility to rhinovirus (RV) infection and RV-induced inflammatory response between the nasal mucosae from patients with chronic rhinosinusitis with nasal polyps (CRS/NP) and subjects without CRS/NP (hereinafter, normal subjects).
  
  
  METHODS
  In vitro study.
  
  
  METHODS
  Tertiary care rhinology clinic.
  
  
  METHODS
  We conducted RV infection experiments on the organ cultures of NPs and inferior turbinate mucosae from 16 patients with CRS/NP and sphenoid sinus and inferior turbinate mucosae from 19 patients who underwent transsphenoidal pituitary surgery.
  
  
  METHODS
  Successful RV-16 infection was determined by positive identification of RV on the surface fluid of organ culture using seminested reverse transcriptase-polymerase chain reaction. Effects of RV on interleukin 6 (IL-6) and IL-8 secretion were measured by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  The successful RV infection was achievable in 9 of 16 NP samples (56.3%) and 9 of 16 turbinate samples (56.3%) from patients with CRS/NP compared with 11 of 19 sphenoid sinus samples (57.9%) and 15 of 19 turbinate samples (78.9%) from normal subjects. The RV infection increased IL-6 and IL-8 secretion 236% and 173%, respectively, in NP samples, and 218% and 178%, respectively, in turbinate samples from patients with CRS/NP; compared with 231% and 145%, respectively, in sphenoid mucosa samples, and 181% and 148%, respectively, in turbinate samples from normal subjects. However, there were no statistical differences among the 4 groups.
  
  
  CONCLUSIONS
  These in vitro findings suggest that subjects with CRS/NP mucosa might not be more susceptible to RV infection, and did not secrete more cytokines in response to rhinovirus infection, than those with normal mucosa.
authors.affiliation: Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Pungnap-Dong, Sonpa-gu, Seoul, South Korea.
authors.forename: Jong Hwan
authors.initials: JH
authors.lastname: Wang
authors.forename: Hyun Ja
authors.initials: HJ
authors.lastname: Kwon
authors.forename: Yoo-Sam
authors.initials: YS
authors.lastname: Chung
authors.forename: Bong-Jae
authors.initials: BJ
authors.lastname: Lee
authors.forename: Yong Ju
authors.initials: YJ
authors.lastname: Jang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D002648:Child; D002908:Chronic Disease; D016207:Cytokines / Q000378:metabolism*; D004198:Disease Susceptibility; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000150:complications* / Q000378:metabolism / Q000821:virology; D010850:Picornaviridae Infections / Q000209:etiology / Q000378:metabolism*; D012220:Rhinitis / Q000150:complications* / Q000378:metabolism / Q000821:virology; D012229:Rhinovirus; D012852:Sinusitis / Q000150:complications* / Q000378:metabolism / Q000821:virology; D046509:Tissue Culture Techniques; D014420:Turbinates / Q000378:metabolism
chemical_list: D016207:Cytokines