title: Construction and assay of radiation hybrids.
abstract: This unit describes the irradiation and fusion of cells to produce radiation hybrids and the harvesting and expansion of RH colonies. A support protocol describes amplification of human chromosome fragments by PCR and analysis of those fragments by agarose gel electrophoresis.
authors.affiliation: University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: C W
authors.initials: CW
authors.lastname: Richard
authors.forename: S S
authors.initials: SS
authors.lastname: Washington
mesh_terms: D000818:Animals; D002459:Cell Fusion; D002877:Chromosomes, Human; D006224:Cricetinae; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D005826:Genetics, Medical; D006801:Humans; D016133:Polymerase Chain Reaction; D021101:Radiation Hybrid Mapping / Q000379:methods*
chemical_list: D005819:Genetic Markers