[SNP discovery by F-CSGE in the coding region of mitochondrial DNA in wild house mice from Shanghai suburb].
This study was performed to discover SNPs for genetic polymorphism analysis of mitochondrial DNA from wild house mice. Universal primer florescent PCR, fluorescence-based conformation sensitive gel electrophoresis (F-CSGE) and DNA sequencing were conducted to analyze the coding region of mitochondrial DNA. Different types of unknown mutations were recorded by variable F-CSGE patterns without false positive. Twenty-four SNPs, sixteen of which were first discovered in the coding region of mitochondrial DNA, were found in 64 wild house mice from 4 districts in Shanghai. Therefore, F-CSGE was proved to be powerful technique for SNP discovery in the coding region mitochondrial DNA. The novel SNPs can be used as molecular markers to analyze population structure and genetic polymorphisms of the wild house mice in Shanghai.
Fan
Zhong-Peng
ZP
Institute of Biology Science & Technology, Donghua University, Shanghai 201620, China. pawffl@163.com
Zhu
Wang-Sheng
WS
Zhang
Chao
C
Zhou
Yu-Xun
YX
Li
Kai
K
Liang
Yin-Ming
YM
Xing
Zheng-Hong
ZH
Chen
Guo-Qiang
GQ
Bai
Xiong
X
Xiao
Jun-Hua
JH
D000818:Animals; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods*; D051379:Mice; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
D004272:DNA, Mitochondrial