pH-cycling models to evaluate the effect of low fluoride dentifrice on enamel de- and remineralization.
Since the currently available pH-cycling models do not differentiate the anti-caries potential of dentifrices with low fluoride (F) concentration, two models were developed and tested in the present. Bovine enamel blocks were subjected to the models and treated with F solutions containing from 70 to 280 microg F/mL in order to validate them in terms of dose-response effect. The models were also tested by evaluating the dentifrices Colgate Baby (500 microg F/g, as a low fluoride dentifrice), Tandy (1,100 microg F/g, as an active F-dentifrice) and Crest (1,100 microg F/g, as positive control). Enamel mineral loss or gain was assessed by surface and cross-sectional microhardness, and lesion depth was analyzed by polarized light microscopy. The pH-cycling models showed F dose-response effect either reducing enamel demineralization or enhancing remineralization. The low F dentifrice presented anti-caries potential, but it was not equivalent to the dentifrices containing 1,100 microg F/g. These data suggest that the models developed in this study were able to evaluate the anti-caries potential of low F dentifrice either on resistance to demineralization or on enhancement of remineralization.
Queiroz
Celso Silva
CS
Department of Physiological Sciences, School of Dentistry of Piracicaba, State University of Campinas, Piracicaba, SP, Brazil.
Hara
Anderson Takeo
AT
Paes Leme
Adriana Franco
AF
Cury
Jaime Aparecido
JA
D000818:Animals; D002118:Calcium / Q000032:analysis; D002327:Cariostatic Agents / Q000008:administration & dosage*; D002417:Cattle; D003743:Dental Enamel / Q000187:drug effects* / Q000648:ultrastructure; D003747:Dental Enamel Solubility / Q000187:drug effects; D003802:Dentifrices / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D005459:Fluorides / Q000008:administration & dosage*; D006244:Hardness; D006863:Hydrogen-Ion Concentration; D008422:Materials Testing; D008859:Microscopy, Polarization; D008903:Minerals / Q000032:analysis; D011897:Random Allocation; D012824:Silicic Acid; D012822:Silicon Dioxide / Q000008:administration & dosage; D012969:Sodium Fluoride / Q000008:administration & dosage; D013696:Temperature; D013997:Time Factors; D017001:Tooth Demineralization / Q000473:pathology / Q000517:prevention & control*; D014089:Tooth Remineralization; D014100:Toothpastes
D002327:Cariostatic Agents; D003802:Dentifrices; D008903:Minerals; D014100:Toothpastes; C546060:hydrated silica gel-based toothpaste; D012824:Silicic Acid; D012822:Silicon Dioxide; D012969:Sodium Fluoride; D005459:Fluorides; D002118:Calcium