title: Thin-layer chromatography.
abstract: TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.
authors.affiliation: Purdue University, West Lafayette, Indiana, USA.
authors.forename: C L
authors.initials: CL
authors.lastname: Meyers
mesh_terms: D002855:Chromatography, Thin Layer / Q000295:instrumentation* / Q000379:methods*; D009696:Nucleic Acids / Q000032:analysis / Q000302:isolation & purification
chemical_list: D009696:Nucleic Acids