[Multi-residue analysis of 10 beta2-agonists in animal tissues using gas chromatography-mass spectrometry].
A method for multi-residue analysis of beta2-agonists, mabuterol, terbutaline, carbuterol, clenbuterol, cimaterol, salbutamol, clenpenterol, isoxsuprine, bambuterol and ractopamine in animal tissues using gas chromatography-mass spectrometry, based on isotope dilution and solid phase extraction has been developed. The homogenized sample was spiked with isotope-labeled internal standards, D9-clenbuterol, D3-salbutamol and D5-ractopamine, extracted with anhydrous alcohol, defatted using hexane, and cleaned-up on an SLS cartridge, and derivatized with N, O-bis (trimethylsilyl) trifluoro acetamide (BSTFA) + 1% trimethylchlorosilane (TMCS). The experimental results indicated that the recoveries were 72.8%-110.3%, and the relative standard deviations were 1.2%-11.3% in blank samples spiked with the above mentioned agonists at 2.0-10.0 microg/kg, and the corresponding detection limits were 0.5-1.0 micro/kg.
Wu
Pinggu
P
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China. pgwu@cdc.zj.cn
Chen
Huihua
H
Wang
Qiang
Q
Ying
Yongfei
Y
Zhao
Yongxin
Y
Song
Guoliang
G
Xu
Xiaoming
X
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000032:analysis*; D000818:Animals; D002138:Calibration; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists