title: Immunoreactive GnRH type I receptors in the mouse and sheep brain.
abstract: Gonadotropin Releasing Hormone-I (GnRH) has been implicated in an array of functions outside the neuroendocrine reproductive axis. Previous investigations have reported extensive GnRH binding in numerous sites and this has been supported by in situ hybridization studies reporting GnRH receptor mRNA distribution. The present study on mice and sheep supports and extends these earlier investigations by revealing the distribution of cells immunoreactive for the GnRH receptor. In addition to sites previously shown to express GnRH receptors such as the hippocampus, amygdala and the arcuate nucleus, the improved resolution afforded by immunocytochemistry detected cells in the mitral cell lay of the olfactory bulb as well as the central grey of the mesencephalon. In addition, GnRH receptor immunoreactive neurons in the hippocampus and mesencephalon of the sheep were shown to colocalize with estrogen receptor beta. Although GnRH may act at some of these sites to regulate reproductive processes, evidence is accumulating to support an extra-reproductive role for this hypothalamic decapeptide.
authors.affiliation: Department of Zoology and Physiology & Neurobiology Program, University of Wyoming, Dept 3166, 1000 E University Avenue, Laramie, WY 82071, USA.
authors.forename: Asher J
authors.initials: AJ
authors.lastname: Albertson
authors.forename: Amy
authors.initials: A
authors.lastname: Navratil
authors.forename: Mallory
authors.initials: M
authors.lastname: Mignot
authors.forename: Laurence
authors.initials: L
authors.lastname: Dufourny
authors.forename: Brian
authors.initials: B
authors.lastname: Cherrington
authors.forename: Donal C
authors.initials: DC
authors.lastname: Skinner
mesh_terms: D000679:Amygdala / Q000166:cytology / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D001931:Brain Mapping; D016466:CHO Cells; D002417:Cattle; D006224:Cricetinae; D003412:Cricetulus; D047629:Estrogen Receptor beta / Q000378:metabolism; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D010487:Periaqueductal Gray / Q000166:cytology / Q000378:metabolism; D011966:Receptors, LHRH / Q000378:metabolism*; D012756:Sheep; D013045:Species Specificity
chemical_list: D047629:Estrogen Receptor beta; D011966:Receptors, LHRH; D007987:Gonadotropin-Releasing Hormone