title: X-ray beam stabilization at BL-17A, the protein microcrystallography beamline of the Photon Factory.
abstract: BL-17A is a new structural biology beamline at the Photon Factory, Japan. The high-brilliance beam, derived from the new short-gap undulator (SGU#17), allows for unique protein crystallographic experiments such as data collection from microcrystals and structural determination using softer X-rays. However, microcrystal experiments require robust beam stability during data collection and minor fluctuations could not be ignored. Initially, significant beam instability was observed at BL-17A. The causes of the beam instability were investigated and its various sources identified. Subsequently, several effective countermeasures have been implemented, and the fluctuation of the beam intensity successfully suppressed to within 1%. Here the instability reduction techniques used at BL-17A are presented.
authors.affiliation: Photon Factory, Structural Biology Research Center, Institute of Materials Structure Science, High Energy Accelerator Research Organization, 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan. noriyuki.igarashi@kek.jp
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Igarashi
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Ikuta
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Naohiro
authors.initials: N
authors.lastname: Matsugaki
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamada
authors.forename: Mohammad S
authors.initials: MS
authors.lastname: Yousef
authors.forename: Soichi
authors.initials: S
authors.lastname: Wakatsuki
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D017785:Photons; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins