Sensitizing effects of chronic exposure and acute inhalation of ovalbumin aerosol on pulmonary C fibers in rats.
The effect of ovalbumin (Ova) sensitization on pulmonary C-fiber sensitivity was investigated. Brown-Norway rats were sensitized by intraperitoneal injection of Ova followed by aerosolized Ova three times per week for 3 wk. Control rats received the vehicle. At the end of the third week, single-unit fiber activities (FA) of pulmonary C fibers were recorded in anesthetized, artificially ventilated rats. Our results showed the following: 1) Ova sensitization induced airway inflammation (infiltration of eosinophils and neutrophils) and airway hyperresponsiveness in rats; 2) baseline FA in sensitized rats was significantly higher than that in control ones; 3) similarly, the pulmonary C-fiber response to right atrial injection of capsaicin was markedly higher in sensitized rats, which were significantly amplified after the acute Ova inhalation challenge; and 4) similar patterns, but smaller magnitudes of the differences in C-fiber responses to adenosine and lung inflation, were also found between sensitized and control rats. In conclusion, Ova sensitization elevated the baseline FA and excitability of pulmonary C fibers, and the hypersensitivity was further potentiated after the acute Ova inhalation challenge in sensitized rats. Chronic allergic inflammatory reactions in the airway probably contributed to the sensitizing effect on these lung afferents.
Zhang
Guangfan
G
Department of Physiology, University of Kentucky Medical Center, 800 Rose St., Lexington, KY 40536-0298, USA.
Lin
Ruei-Lung
RL
Wiggers
Michelle E
ME
Lee
Lu-Yuan
LY
D000280:Administration, Inhalation; D000336:Aerosols; D000485:Allergens / Q000008:administration & dosage / Q000276:immunology; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects / Q000502:physiology; D016535:Bronchial Hyperreactivity / Q000503:physiopathology; D016085:Bronchoconstrictor Agents / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006339:Heart Rate / Q000187:drug effects / Q000502:physiology; D006967:Hypersensitivity / Q000276:immunology*; D007249:Inflammation / Q000473:pathology; D008168:Lung / Q000294:innervation* / Q000502:physiology; D008297:Male; D016210:Methacholine Chloride / Q000494:pharmacology; D036421:Nerve Fibers, Unmyelinated / Q000502:physiology*; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology*; D051381:Rats; D011914:Rats, Inbred BN
D000336:Aerosols; D000485:Allergens; D016085:Bronchoconstrictor Agents; D016210:Methacholine Chloride; D010047:Ovalbumin