title: cDNA library construction from single cells.
abstract: Analysis of gene expression or comparing gene expression patterns between cells and/or tissues facilitates the identification of molecules activated by a cellular program or by a particular physiological or pharmacological treatment. This unit presents a protocol for studying these molecules at the level of a single cell.
authors.affiliation: NIH, NINDS, CDNS, Bethesda, Maryland, USA.
authors.forename: Phillip R
authors.initials: PR
authors.lastname: Kramer
mesh_terms: D000818:Animals; D002469:Cell Separation / Q000379:methods*; D003001:Cloning, Molecular / Q000379:methods; D018076:DNA, Complementary / Q000032:analysis / Q000235:genetics*; D015870:Gene Expression / Q000235:genetics; D015723:Gene Library; D006801:Humans
chemical_list: D018076:DNA, Complementary