Intracellular and juxtacellular staining with biocytin.
Many physiological studies require microscopic examination of the recorded neuron for identification. This unit describes how intracellular and extracellular recording can be combined with single-neuron staining to enable sequential physiological and morphological studies.
Wilson
Charles J
CJ
The University of Texas at San Antonio, San Antonio, Texas, USA.
Sachdev
R N S
RN
D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives; D004563:Electrochemistry / Q000295:instrumentation; D004566:Electrodes / Q000145:classification; D004594:Electrophysiology / Q000379:methods; D004867:Equipment Design; D005110:Extracellular Space / Q000378:metabolism; D006801:Humans; D007425:Intracellular Membranes / Q000378:metabolism; D008239:Lysine / Q000031:analogs & derivatives*; D009445:Neuroanatomy / Q000379:methods*; D009448:Neurochemistry / Q000379:methods*; D009474:Neurons / Q000378:metabolism*; D009482:Neurophysiology / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*
C070614:neurobiotin; D001710:Biotin; C013411:biocytin; D008239:Lysine