Antibodies against Epstein-Barr virus gp78 antigen: a novel marker for serological diagnosis of nasopharyngeal carcinoma detected by xMAP technology.
Immunoglobulin (Ig) A and/or IgG reactivities to several Epstein-Barr virus (EBV) antigens have been used to facilitate diagnosis of nasopharyngeal carcinoma (NPC). However, antibodies against gp78, an EBV membrane glycoprotein, have not been examined to this day. In this study, we utilized Luminex multi-analyte profiling (xMAP) technology to analyse antibody responses to a synthetic peptide of gp78 in sera samples from 95 NPC patients and 91 healthy controls. Our results showed the sensitivity and specificity of IgA-gp78 for NPC diagnosis were 79 and 71 %, respectively, while those of IgG-gp78 were 74 and 73 %, respectively. The IgA and IgG responses to different EBV antigens were not identical within an individual and IgA-gp78 and IgG-gp78 could be complementary to antibodies against viral capsid antigen (VCA), the diffused early antigen (EA-D) and the nuclear antigen EBNA1 for NPC diagnosis. When the six EBV parameters for NPC prediction, i.e. IgA-gp78, IgG-gp78, IgA-VCA, IgA-EBNA1, IgA-EA-D and IgG-EA-D, are combined, the combined predictors were able to reach overall sensitivity and specificity of 91 and 95 %, respectively. Thus, simultaneous detection of these EBV serological markers could improve the predictive values of NPC using xMAP technology.
Gu
Ai-Di
AD
State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.
Xie
Yan-Bo
YB
State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.
Mo
Hao-Yuan
HY
State Key Laboratory of Oncology in Southern China; Department Nasopharyngeal Carcinoma, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.|State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.
Jia
Wei-Hua
WH
State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.
Li
Miao-Yan
MY
Da'an Gene Diagnostic Center, Sun Yat-Sen University, Guangzhou, PR China.
Li
Ming
M
Da'an Gene Diagnostic Center, Sun Yat-Sen University, Guangzhou, PR China.
Chen
Li-Zhen
LZ
State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.
Feng
Qi-Sheng
QS
State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.
Liu
Quentin
Q
State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.
Qian
Chao-Nan
CN
State Key Laboratory of Oncology in Southern China; Department Nasopharyngeal Carcinoma, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.|State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.
Zeng
Yi-Xin
YX
State Key Laboratory of Oncology in Southern China, Department of Experimental Research, Cancer Center, Sun Yat-Sen University, Guangzhou, PR China.
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000914:Antibodies, Viral / Q000097:blood*; D000956:Antigens, Viral / Q000276:immunology*; D015415:Biomarkers; D002277:Carcinoma / Q000175:diagnosis*; D004854:Herpesvirus 4, Human / Q000276:immunology*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D014763:Viral Matrix Proteins / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D015415:Biomarkers; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D014763:Viral Matrix Proteins