Serum amyloid A: a typical acute-phase reactant in rainbow trout?
Acute serum amyloid A (A-SAA) has been considered a major acute-phase reactant and an effector of innate immunity in all vertebrates. The work presented here shows that the expression of A-SAA is strongly induced in a wide variety of immune-relevant tissues in rainbow trout, either naturally infected with Flavobacterium psychrophilum or challenged with lipopolysaccharide (LPS) or CpG oligonucleotides (CpG ODN). Nevertheless, A-SAA was undetectable by Western blot either in the plasma or in high-density lipoprotein (HDL) of infected or challenged fish, using either an anti-mouse SAA1 IgG or an anti-trout A-SAA peptide serum, which recognise both the intact recombinant trout A-SAA and fragments derived from it. However, the anti-peptide serum was the immunoreactive in all primary defence barriers and in mononuclear cells of head kidney, spleen and liver. These findings reveal that, unlike mammalian SAA, trout A-SAA does not increase significantly in the plasma of diseased fish, suggesting it is more likely to be involved in local defence.
Villarroel
Franz
F
Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile. franzvillarroel@uach.cl
Casado
Alin
A
Vásquez
Jorge
J
Matamala
Ella
E
Araneda
Bruno
B
Amthauer
Rodolfo
R
Enriquez
Ricardo
R
Concha
Margarita I
MI
D000210:Acute-Phase Reaction / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies / Q000276:immunology; D017124:Conserved Sequence; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D017686:Oncorhynchus mykiss / Q000097:blood* / Q000235:genetics / Q000276:immunology*; D016415:Sequence Alignment; D000685:Serum Amyloid A Protein / Q000737:chemistry / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects
D000906:Antibodies; D008070:Lipopolysaccharides; D000685:Serum Amyloid A Protein