title: Biotinylation and analysis of membrane-bound and soluble proteins.
abstract: Coupling of a biotin moiety to proteins allows sensitive detection of those proteins with a variety of commercially available avidin or streptavidin conjugates. This unit presents protocols for biotinylation of cell-surface proteins and proteins in solution. A major advantage of these techniques is that they do not require use of radioisotopes (although use of radioiodinated streptavidin is optional for biotin detection).
authors.affiliation: Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Lisanti
authors.forename: M
authors.initials: M
authors.lastname: Sargiacomo
mesh_terms: D001360:Avidin / Q000737:chemistry; D001710:Biotin / Q000737:chemistry*; D008565:Membrane Proteins / Q000032:analysis* / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012995:Solubility; D019809:Streptavidin / Q000737:chemistry
chemical_list: D008565:Membrane Proteins; D001360:Avidin; D001710:Biotin; D019809:Streptavidin