The expression of sialylated high-antennary N-glycans in edible bird's nest.
Edible bird's nest (EBN) is the nest made from the saliva of Collocalia swift. Recently, we have found that EBN extract could strongly inhibit infection of influenza viruses in a host-range-independent manner [Guo, C. T.; Takahashi, T.; Bukawa, W.; Takahashi, N.; Yagi, H.; Kato, K.; Hidari, K. I.; Miyamoto, D.; Suzuki, T.; Suzuki, Y. Antiviral Res.2006, 70, 140-146]. Although this antiviral activity might be attributed to O- or N-glycoconjugates, no N-glycan structures have so far been described for EBN. Here, we report the N-glycosylation profile of EBN, in which a tri-antennary N-glycan bearing the alpha2,3-N-acetylneuraminic acid residues is displayed as a major component. We suggest that the sialylated high-antennary N-glycans of EBN contribute to the inhibition of influenza viral infection.
Yagi
Hirokazu
H
Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
Yasukawa
Naoko
N
Yu
Shin-Yi
SY
Guo
Chao-Tan
CT
Takahashi
Noriko
N
Takahashi
Tadanobu
T
Bukawa
Wakoto
W
Suzuki
Takashi
T
Khoo
Kay-Hooi
KH
Suzuki
Yasuo
Y
Kato
Koichi
K
D000818:Animals; D001717:Birds; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006031:Glycosylation; D008297:Male; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000096:biosynthesis* / Q000737:chemistry; D025942:Oligosaccharides, Branched-Chain / Q000096:biosynthesis* / Q000737:chemistry; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry
D025942:Oligosaccharides, Branched-Chain; D011134:Polysaccharides; D019158:N-Acetylneuraminic Acid