title: Immunomagnetic separation combined with real-time reverse transcriptase PCR assays for detection of norovirus in contaminated food.
abstract: We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants.
authors.affiliation: Department of Environmental Health, School of Public Health, Seoul National University, 28 Yunkeun-Dong, Chongro-Ku, Seoul 110-799, South Korea.
authors.forename: YoungBin
authors.initials: Y
authors.lastname: Park
authors.forename: You-Hee
authors.initials: YH
authors.lastname: Cho
authors.forename: YoungMee
authors.initials: Y
authors.lastname: Jee
authors.forename: GwangPyo
authors.initials: G
authors.lastname: Ko
mesh_terms: D005506:Food Contamination; D031985:Fragaria / Q000382:microbiology*; D018189:Immunomagnetic Separation / Q000379:methods*; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase; D013997:Time Factors
chemical_list: D019914:Taq Polymerase