[Effect of dizocilpine maleate and taurine on the circuitry of glutamate and glutamine in rat striatum exposed by manganese].
OBJECTIVE
To observe the effect of MK-801 and taurine on the activities of GS and PAG and the glutamatic neuron content in the rat striatum exposed by manganese.


METHODS
40 Wistar rats were random divided into four groups. The first group was the control group which was subcutaneously injected at the content of 0.9% NaCl. The second group was MnCl2 group which was subcutaneously injected at the content of 0.9% NaCl. The third and fourth groups were pretreatment groups which were subcutaneously injected of 0.3 micromol/kg MK-801 and 1 mmol/kg taurine. After 2h, the first group was peritoneally injected at the content of 0.9% NaCl, the 2nd-4th group were peritoneally injected at the dose of 200 micromol/kg MnCl2. All administration was given at the dose of 5 ml/kg for 25d, the pretreatment groups were given for every other day. At the 24h after the administration of MnCl2, the 4 rats brain tissue in every groups were removed after the rats were perfused from the left ventricles to with 4% ploymerisatum. The glutamatic neuron content were determined by immunohistochemical method (SABC). The activities of GS and PAG in the residual rat striatum were determined.


RESULTS
In comparison with control group, the percentage of positive area and integral optical densities of glutamate immunocreative cell increased significantly in MnCl2 group (P < 0.01), the activity of GS decreased significantly in MnCl2 group, the activity of PAG increased significantly in MnCl2 group. In comparison with MnCl2 group, the percentage of positive area and integral optical density of glutamate immunocreative cell decreased significantly in MK-801 and taurine pretreatment group, the activity of GS increased significantly in MK-801 and taurine pretreatment group , the activity of PAG decreased significantly in MK-801 pretreatment group.


CONCLUSIONS
MK-801 and taurine have a certain protective effect on "the circuitry of glutamate and glutamine" disrupted with Mn.
Jia
Ke
K
School of Public Health,China Medical University, Shenyang 110001, China. jake981005@126.com
Xu
Zhaofa
Z
Xu
Bin
B
D000818:Animals; D002712:Chlorides / Q000633:toxicity; D003342:Corpus Striatum / Q000187:drug effects / Q000378:metabolism*; D016291:Dizocilpine Maleate / Q000494:pharmacology*; D004785:Environmental Pollutants / Q000633:toxicity; D005260:Female; D018698:Glutamic Acid / Q000378:metabolism*; D005973:Glutamine / Q000378:metabolism*; D008297:Male; D008345:Manganese / Q000633:toxicity*; D017895:Manganese Compounds; D018696:Neuroprotective Agents / Q000494:pharmacology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013654:Taurine / Q000494:pharmacology*
D002712:Chlorides; D004785:Environmental Pollutants; D017895:Manganese Compounds; D018696:Neuroprotective Agents; D005973:Glutamine; D013654:Taurine; D018698:Glutamic Acid; D008345:Manganese; D016291:Dizocilpine Maleate; C025340:manganese chloride