title: Alternative pathway evaluation.
abstract: The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.
authors.affiliation: National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado, USA.
authors.forename: P C
authors.initials: PC
authors.lastname: Giclas
mesh_terms: D000818:Animals; D011415:Complement Factor B / Q000032:analysis; D011416:Complement Factor D / Q000032:analysis; D015941:Complement Hemolytic Activity Assay / Q000379:methods*; D003170:Complement Pathway, Alternative; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D016014:Linear Models; D011336:Probability; D011414:Properdin / Q000032:analysis; D011817:Rabbits; D012680:Sensitivity and Specificity
chemical_list: D011414:Properdin; D011416:Complement Factor D; D011415:Complement Factor B