title: Comparative bioinformatics for mouse and human genes: getting started.
abstract: The primary goal of this unit is to describe methods for using online bioinformatic resources to identify data pertaining to specific mouse genes or genomic regions, from the perspective of a researcher who has identified a human gene(s) of interest and wishes to obtain some basic information about the orthologous murine gene-such as the mouse genomic sequence containing the gene, cDNA sequence and exon/intron structure, and conserved noncoding regions that may indicate regulatory elements. Basic Protocol 1 describes how to obtain mouse gene sequences via Ensembl. Basic Protocol 2 describes a sequence homology search for locating a mouse ortholog of a human gene using the UCSC genome browser. Basic Protocols 3 and 4 provide instructions for performing comparative analysis of large human and mouse genomics sequences, using the PipMaker (Schwartz et al., 2000) and VISTA (Mayor et al., 2000) online tools.
authors.affiliation: Vanderbilt University Medical Center, Nashville, Tennessee, USA.
authors.forename: Douglas P
authors.initials: DP
authors.lastname: Mortlock
mesh_terms: D000818:Animals; D019295:Computational Biology; D030541:Databases, Genetic; D005091:Exons; D005796:Genes; D006801:Humans; D051379:Mice; D009154:Mutation