Accumulation of flavonols in response to ultraviolet-B irradiation in soybean is related to induction of flavanone 3-beta-hydroxylase and flavonol synthase.
There are several branch points in the flavonoid synthesis pathway starting from chalcone. Among them, the hydroxylation of flavanone is a key step leading to flavonol and anthocyanin. The flavanone 3-beta-hydroxylase (GmF3H) gene was cloned from soybean (Glycine max cultivar Sinpaldal) and shown to convert eriodictyol and naringenin into taxifolin and dihydrokaempferol, respectively. The major flavonoids in this soybean cultivar were found by LC-MS/MS to be kamepferol O-triglycosides and O-diglycosides. Expression of GmF3H and flavonol synthase (GmFLS) was induced by ultraviolet-B (UV-B) irradiation and their expression stimulated accumulation of kaempferol glycones. Thus, GmF3H and GmFLS appear to be key enzymes in the biosynthesis of the UV-protectant, kaempferol.
Kim
Bong Gyu
BG
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Seoul 134-901, Korea.
Kim
Jeong Ho
JH
Kim
Jiyoung
J
Lee
Choonghwan
C
Ahn
Joong-Hoon
JH
D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004790:Enzyme Induction; D044948:Flavonols / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D006899:Mixed Function Oxygenases / Q000096:biosynthesis* / Q000235:genetics; D010088:Oxidoreductases / Q000096:biosynthesis* / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000378:metabolism; D013025:Glycine max / Q000201:enzymology* / Q000235:genetics / Q000528:radiation effects*; D013379:Substrate Specificity; D053719:Tandem Mass Spectrometry; D014466:Ultraviolet Rays
D044948:Flavonols; D010940:Plant Proteins; D011994:Recombinant Proteins; D006899:Mixed Function Oxygenases; D010088:Oxidoreductases; C048797:flavanone 3-dioxygenase; C085313:flavonol synthase