title: Marrow-derived mesenchymal stem cells-directed bone regeneration in the dog mandible: a comparison between biphasic calcium phosphate and natural bone mineral.
abstract: OBJECTIVE
  This study was designed to compare mesenchymal stem cell (MSC)-based alveolar bone regeneration in biphasic bone substitutes and natural bone mineral in a canine full-thickness alveolar defect model.
  
  
  METHODS
  MSCs were isolated from bone marrow aspirates and culture expanded through 3 successive subcultures. The bone differentiation potential of third passage cells was evaluated and confirmed in vitro before cells were used in the transplantation experiment. Undifferentiated cells were then incubated with 3 x 3 x 3 mm(3) hydroxyapatite/beta-tricalcium phosphate (HA/TCP) matrices (Kasios, Lanauguet, France) and 1- to 2-mm Bio-Oss spongiosa (Geistlich Biomaterials, Osteohealth, Switzerland), which is a natural bovine bone mineral (NBM). Kasios/cell, Kasios alone, Bio-Oss/cell, and Bio-Oss alone were implanted in masseter muscle and 4 cylindrical (10-mm diameter) through-and-through bilateral mandibular body defects in 4 mongrel dogs. Histomorphometric analysis was performed 6 weeks after insertion of the scaffold loaded with MSCs.
  
  
  RESULTS
  H&E staining of the decalcified scaffold and scanning electron microscopy demonstrated large MSC coverage of the HA/TCP and Bio-Oss. Cell-loaded Kasios matrices showed the greatest amount of the bone regeneration among the groups in both the muscle (29.11%) and the bone specimens (65.78%). Cell-free biphasic scaffold revealed 44.9% bone fill in bone defects and 23.55% in muscle specimen, and Bio-Oss alone matrices had the least amount of new bone formation: 36.84% and 24.16% in bone and muscle specimens respectively. Kasios loaded with MSCs demonstrated more bone regeneration than Bio-Oss/cell but there was no significant statistical difference (P > .05).
  
  
  CONCLUSIONS
  New biphasic synthetic bone substitutes may offer better conditions for bone regeneration than traditional bone substitute in combination with MSCs. They remained in the defect and contributed to bone regeneration.
authors.affiliation: Oral and Maxillofacial Surgery, Department of Oral and Maxillofacial Surgery, Taleghani University Hospital, Iranian Center for Dental Research, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jafarian
authors.forename: Mohamadreza Baghaban
authors.initials: MB
authors.lastname: Eslaminejad
authors.forename: Arash
authors.initials: A
authors.lastname: Khojasteh
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Mashhadi Abbas
authors.forename: Mohammad Mehdi
authors.initials: MM
authors.lastname: Dehghan
authors.forename: Rahele
authors.initials: R
authors.lastname: Hassanizadeh
authors.forename: Bahar
authors.initials: B
authors.lastname: Houshmand
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D001861:Bone Regeneration; D018786:Bone Substitutes; D016025:Bone Transplantation; D002130:Calcium Phosphates; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002478:Cells, Cultured; D003648:Decalcification Technique; D004285:Dogs; D017886:Durapatite; D008334:Mandible / Q000601:surgery; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D008903:Minerals; D010006:Osteoblasts / Q000502:physiology; D054457:Tissue Scaffolds
chemical_list: C077540:Bio-Oss; D018786:Bone Substitutes; D002130:Calcium Phosphates; D008903:Minerals; C485817:beta-tricalcium phosphate; D017886:Durapatite