Synthesis and DNA cleaving activity of water-soluble non-conjugated thienyl tetraynes.
Water-soluble non-conjugated thienyl tetraynes (3-6) were synthesized and their DNA cleaving activity was evaluated using electrophoresis, atomic force microscopy (AFM) and Escherichia coli (E. coli) transformation techniques. The amino-functionalized compound 4 was shown to possess an activity to cleave plasmid DNA by both electrophoresis and E. coli transformation techniques. AFM also showed a cleavage of the circular DNA into a linear form with a formation of burst-star-shaped architectures, which were envisaged to be cross-linked DNA oligomers.
Torikai
Kohei
K
The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka, Ibaraki, Osaka 567-0047, Japan. torikai@cbl.rcast.u-tokyo.ac.jp
Otsuka
Yoichi
Y
Nishimura
Makoto
M
Sumida
Megumi
M
Kawai
Tomoji
T
Sekiguchi
Kiyotoshi
K
Ueda
Ikuo
I
D000480:Alkynes / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D018625:Microscopy, Atomic Force / Q000379:methods; D008968:Molecular Conformation; D012995:Solubility; D013237:Stereoisomerism; D013329:Structure-Activity Relationship; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D014169:Transformation, Bacterial / Q000187:drug effects / Q000502:physiology; D014867:Water / Q000737:chemistry
D000480:Alkynes; D013438:Sulfhydryl Compounds; D014867:Water; D004247:DNA