title: Selection against glycosylation in ruminant pancreatic ribonucleases by replacements in the ancestral carbohydrate attachment site.
abstract: A hypothesis, proposed 25 years ago, that there is selection against glycosylation in ruminant pancreatic ribonucleases by replacement of methionine to leucine in the ancestral carbohydrate attachment site Asn-Met-Thr at residues 34-36, was experimentally confirmed. The replacement of leucine at position 35 by methionine in bovine ribonuclease resulted in a three-fold relative increase in glycosylation when expressed in Chinese hamster ovary cells.
authors.affiliation: Institute of Molecular Biology and Biophysics, Schafmattstrasse 20, ETH-Hönggerberg, HPKE 14, 8093, Zurich, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Gautschi
authors.forename: Jaap J
authors.initials: JJ
authors.lastname: Beintema
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D016466:CHO Cells; D050260:Carbohydrate Metabolism; D002417:Cattle / Q000235:genetics*; D006224:Cricetinae; D003412:Cricetulus; D019143:Evolution, Molecular; D006023:Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D012259:Ribonuclease, Pancreatic / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D006023:Glycoproteins; D012259:Ribonuclease, Pancreatic