Imaging GABAc receptors with ligand-conjugated quantum dots.
We report a methodology for labeling the GABAc receptor on the surface membrane of intact cells. This work builds upon our earlier work with serotonin-conjugated quantum dots and our studies with PEGylated quantum dots to reduce nonspecific binding. In the current approach, a PEGylated derivative of muscimol was synthesized and attached via an amide linkage to quantum dots coated in an amphiphilic polymer derivative of a modified polyacrylamide. These conjugates were used to image GABA(C) receptors heterologously expressed in Xenopus laevis oocytes.
Tomlinson
Ian D
ID
Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.
Gussin
Hélène A
HA
Little
Deborah M
DM
Warnement
Michael R
MR
Qian
Haohua
H
Pepperberg
David R
DR
Rosenthal
Sandra J
SJ