[Hight resoluton optical microscopy of the cellular cycle in psoriasis patients].
Skin tissue from patients with Psoriasis was analyzed using HROM (High Resolution Optical Microscopy), studying epithelial differentiation and possible structural alterations of the queratinocytes. The samples were taken from 10 patients with histopathologic diagnosis of Psoriasis. This tissue samples where affixed with glutaraldehide buffer-collidine for 48 hours. Later processed with the HROM technique and colored with toluidine blue, metilene blue, basic Fuscine, and silver metenamine. The basal epithelial elements presented ovoid nucleus and most of them had prominent nucleolus. In 7 of the studied cases, the granulose stratus was absent, and thinner in the rest, with nucleus and nucleolus retention. At this level queratinocytes where observed with perinuclear anfofilia, as well as linfocitic and macrophagic infiltrate and union complex where elongated.
Frede
S
S
Departamento de Microscopía Electrónica, Hospital Nacional de Clínicas.
Reinoso
N
N
Berrotarán
N
N
Juri
G
G
Piccinni
D J
DJ
Asis
G
G
Burgos
E
E
Spitale
L S
LS
D015703:Antigens, CD / Q000032:analysis; D004396:Coloring Agents; D004847:Epithelial Cells / Q000648:ultrastructure*; D005109:Extracellular Matrix / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000473:pathology; D008853:Microscopy / Q000379:methods; D055095:Optics and Photonics; D011565:Psoriasis / Q000473:pathology*; D012867:Skin / Q000473:pathology*; D013601:T-Lymphocytes / Q000648:ultrastructure
D015703:Antigens, CD; D004396:Coloring Agents