title: Production of monoclonal antibodies.
abstract: This unit describes the production of monoclonal antibodies beginning with immunization and cell fusion and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY).
authors.affiliation: Washington University School of Medicine, St. Louis, Missouri.
authors.forename: Wayne M
authors.initials: WM
authors.lastname: Yokoyama
authors.affiliation: StemCell Technologies, Inc, Vancouver, British Columbia, Canada.
authors.forename: Michelle
authors.initials: M
authors.lastname: Christensen
authors.affiliation: StemCell Technologies, Inc, Vancouver, British Columbia, Canada.
authors.forename: Gary Dos
authors.initials: GD
authors.lastname: Santos
authors.affiliation: StemCell Technologies, Inc, Vancouver, British Columbia, Canada.
authors.forename: Diane
authors.initials: D
authors.lastname: Miller
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D000918:Antibody Specificity; D002459:Cell Fusion; D002460:Cell Line; D002999:Clone Cells / Q000276:immunology; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D006825:Hybridomas / Q000166:cytology / Q000276:immunology*; D007114:Immunization; D051379:Mice
chemical_list: D000911:Antibodies, Monoclonal; D017077:Culture Media, Conditioned