title: HPLC of peptides and proteins.
abstract: High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This is an excellent primer for HPLC users.
authors.affiliation: Monash University, Victoria, Australia.
authors.forename: Reinhard I
authors.initials: RI
authors.lastname: Boysen
authors.affiliation: Monash University, Victoria, Australia.
authors.forename: Milton T W
authors.initials: MTW
authors.lastname: Hearn
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results
chemical_list: D010455:Peptides; D011506:Proteins