title: Hybridization analysis of DNA blots.
abstract: Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. This appendix describes restriction endonucleases and their properties.
authors.affiliation: University of Manchester Institute of Science and Technology, Manchester, United Kingdom.
authors.forename: T
authors.initials: T
authors.lastname: Brown
authors.forename: K
authors.initials: K
authors.lastname: Struhl
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000201:enzymology; D015139:Blotting, Southern / Q000379:methods*; D004247:DNA / Q000032:analysis / Q000302:isolation & purification; D015342:DNA Probes / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases; D008567:Membranes, Artificial; D009691:Nucleic Acid Denaturation; D009693:Nucleic Acid Hybridization / Q000379:methods*; D009711:Nucleotides; D011401:Promoter Regions, Genetic / Q000235:genetics; D011868:Radioisotopes; D012474:Salmon; D012996:Solutions
chemical_list: D015342:DNA Probes; D008567:Membranes, Artificial; D009711:Nucleotides; D011868:Radioisotopes; D012996:Solutions; D004247:DNA; D012321:DNA-Directed RNA Polymerases