Analysis of oxidized DNA fragments by gel electrophoresis.
Polyacrylamide gel electrophoresis is used to define and quantify products of deoxyribose oxidation in DNA, based on the unique electrophoretic mobility of DNA fragments possessing deoxyribose oxidation products on their termini. This approach allows initial estimation of the chemistry. Once the chemical identity of damage products has been confirmed, this technique allows sensitive quantitation of the various damage products.
Awada
M
M
Massachusetts Institute of Technlogy, Cambridge, Massachusetts, USA.
Dedon
P C
PC
D000818:Animals; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D003855:Deoxyribose / Q000032:analysis / Q000737:chemistry; D004586:Electrophoresis / Q000379:methods*; D010084:Oxidation-Reduction
D003855:Deoxyribose; D004247:DNA; C427203:calf thymus DNA