title: The expression of sialylated high-antennary N-glycans in edible bird's nest.
abstract: Edible bird's nest (EBN) is the nest made from the saliva of Collocalia swift. Recently, we have found that EBN extract could strongly inhibit infection of influenza viruses in a host-range-independent manner [Guo, C. T.; Takahashi, T.; Bukawa, W.; Takahashi, N.; Yagi, H.; Kato, K.; Hidari, K. I.; Miyamoto, D.; Suzuki, T.; Suzuki, Y. Antiviral Res.2006, 70, 140-146]. Although this antiviral activity might be attributed to O- or N-glycoconjugates, no N-glycan structures have so far been described for EBN. Here, we report the N-glycosylation profile of EBN, in which a tri-antennary N-glycan bearing the alpha2,3-N-acetylneuraminic acid residues is displayed as a major component. We suggest that the sialylated high-antennary N-glycans of EBN contribute to the inhibition of influenza viral infection.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagoya City University, Nagoya, Japan.
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Yagi
authors.forename: Naoko
authors.initials: N
authors.lastname: Yasukawa
authors.forename: Shin-Yi
authors.initials: SY
authors.lastname: Yu
authors.forename: Chao-Tan
authors.initials: CT
authors.lastname: Guo
authors.forename: Noriko
authors.initials: N
authors.lastname: Takahashi
authors.forename: Tadanobu
authors.initials: T
authors.lastname: Takahashi
authors.forename: Wakoto
authors.initials: W
authors.lastname: Bukawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Suzuki
authors.forename: Kay-Hooi
authors.initials: KH
authors.lastname: Khoo
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Koichi
authors.initials: K
authors.lastname: Kato
mesh_terms: D000818:Animals; D001717:Birds; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D006031:Glycosylation; D008297:Male; D008969:Molecular Sequence Data; D019158:N-Acetylneuraminic Acid / Q000096:biosynthesis* / Q000737:chemistry; D025942:Oligosaccharides, Branched-Chain / Q000096:biosynthesis* / Q000737:chemistry; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry
chemical_list: D025942:Oligosaccharides, Branched-Chain; D011134:Polysaccharides; D019158:N-Acetylneuraminic Acid