title: RNAi: design and analysis.
abstract: RNAi is a novel genetic tool that is poised to revolutionize the study of genetics and gene networks. Use of this tool requires knowledge of the underlying mechanisms and application of this knowledge to the construction of a gene-silencing library. This unit discusses approaches to rational design of this library and its use in experiments. It focuses on the design algorithms developed so far and briefly discuss several issues that could lead to improvements in the current design algorithms.
authors.affiliation: Sachidanandam Lab, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, USA.
authors.forename: Ravi
authors.initials: R
authors.lastname: Sachidanandam
mesh_terms: D001483:Base Sequence; D003628:Database Management Systems; D030541:Databases, Genetic; D015723:Gene Library; D008969:Molecular Sequence Data; D034622:RNA Interference; D015347:RNA Probes / Q000235:genetics*; D034741:RNA, Small Interfering / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods*
chemical_list: D015347:RNA Probes; D034741:RNA, Small Interfering