Evaluation of ISCOM-adjuvanted subunit vaccines containing recombinant feline immunodeficiency virus Rev, OrfA and envelope protein in cats.
For the development of feline immunodeficiency virus (FIV) vaccines mostly structural proteins have been evaluated for their capacity to induce protective immunity. In the present study, subunit vaccines containing recombinant FIV accessory proteins Rev and OrfA were evaluated in cats. Cats were vaccinated repeatedly with these proteins, adjuvanted with immune stimulating complexes (ISCOMs). In addition, cats were vaccinated with bacterially expressed fragments spanning the entire FIV envelope protein, either alone or in combination with the regulatory proteins. Subsequently, the cats were challenged with a homologous FIV strain to assess the level of protective immunity achieved with the respective vaccination regimens. Although the vaccines proved to be immunogenic, vaccinated cats were not protected from infection with FIV.
Huisman
Willem
W
Erasmus MC, Institute of Virology, Rotterdam, The Netherlands.
Schrauwen
Eefje J A
EJ
Pas
Suzan D
SD
van Amerongen
Geert
G
Rimmelzwaan
Guus F
GF
Osterhaus
Albert D M E
AD
D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016516:CD4-CD8 Ratio; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002415:Cats; D016181:Feline Acquired Immunodeficiency Syndrome / Q000517:prevention & control; D015694:Gene Products, rev / Q000276:immunology*; D006023:Glycoproteins / Q000276:immunology*; D016882:ISCOMs / Q000008:administration & dosage*; D016089:Immunodeficiency Virus, Feline / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood; D009500:Neutralization Tests; D011994:Recombinant Proteins / Q000276:immunology; D022223:Vaccines, Subunit / Q000276:immunology; D014759:Viral Envelope Proteins / Q000276:immunology*; D019562:Viral Load; D014764:Viral Proteins / Q000276:immunology*
D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D015694:Gene Products, rev; D006023:Glycoproteins; D016882:ISCOMs; D007074:Immunoglobulin G; C514198:ORF-A protein, feline immunodeficiency virus; D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014759:Viral Envelope Proteins; D014764:Viral Proteins; C445079:envelope protein, Feline immunodeficiency virus