Heterologous expression of metK1-sp and afsR-sp in Streptomyces venezuelae for the production of pikromycin.
Two regulator genes, metK1-sp and afsR-sp, from Streptomyces peucetius ATCC 27952 were heterologously expressed in S. venezuelae ATCC 15439, to produce 14-membered pikromycin antibiotics. The production of pikromycin was increased by 1.6-fold and 2.6-fold by the expression of metK1-sp and afsR-sp, respectively. The overexpression of metK1-sp and afsR-sp in S. venezuelae stimulated the expression of the pathway-specific regulatory genes, pikD and ketosynthase, as demonstrated by RT-PCR. The elevated transcripts of the pikD and ketosynthase genes were consistent with the enhanced production of pikromycin.
Maharjan
Sushila
S
Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University, #100, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam 336-708, Republic of Korea.
Oh
Tae-Jin
TJ
Lee
Hei Chan
HC
Sohng
Jae Kyung
JK
D002851:Chromatography, High Pressure Liquid; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D018942:Macrolides / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D010957:Plasmids / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013302:Streptomyces / Q000235:genetics* / Q000254:growth & development
D018942:Macrolides; C003145:picromycin