title: [Effect of smoking on neutrophil oxidative metabolism].
abstract: Alterations in neutrophil function by tobacco products may play a central role in the pathogenesis of periodontal diseases and several smoking-related systemic diseases. The aim of the study was to evaluate the effect of smoking on neutrophil oxidative metabolism.
  
  
  METHODS
  The study included 17 smoking men free of systemic diseases who were referred for treatment of various odontological diseases to outpatient department of Kaunas University of Medicine Hospital. The age of subjects varied from 22 to 43 years. All subjects answered the questions about smoking habits. Clinical examination included assessment of oral hygiene status according to the OHI-s index and periodontal status according to Russell and Ramfjord indices. To evaluate the oxidative metabolism of neutrophils, luminol- and liucigenin-dependent chemiluminescence and nitroblue tetrazolium test were used.
  
  
  RESULTS
  After smoking, extracellular liucigenin-dependent chemiluminescence response was higher as compared to the response before smoking, but total (intra- and extracellular) luminol-dependent chemiluminescence response was the same both before and after smoking. Exposure of neutrophils to smoking caused a significant increase in nitroblue tetrazolium reduction.
  
  
  CONCLUSIONS
  The release of reactive oxygen species in neutrophils exposed to smoking may alter the pathogenic processes in periodontal diseases.
authors.affiliation: Kauno medicinos universiteto Dantu ir zandikauliu ortopedijos klinika.
authors.forename: Darius
authors.initials: D
authors.lastname: Zasimauskas
authors.forename: Gediminas
authors.initials: G
authors.lastname: Zekonis
mesh_terms: D000328:Adult; D003627:Data Interpretation, Statistical; D006801:Humans; D007202:Indicators and Reagents; D049449:Luminescence; D008297:Male; D008954:Models, Biological; D009504:Neutrophils / Q000378:metabolism*; D009580:Nitroblue Tetrazolium; D009911:Oral Hygiene Index; D010510:Periodontal Diseases / Q000209:etiology*; D010512:Periodontal Index; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012907:Smoking / Q000009:adverse effects*; D011795:Surveys and Questionnaires
chemical_list: D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D009580:Nitroblue Tetrazolium