Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors.
Fluorescence resonance energy transfer (FRET) with fluorescent proteins is a powerful method for detection of protein-protein interactions, enzyme activities and small molecules in the intracellular milieu. Aided by a new violet-excitable yellow-fluorescing variant of Aequorea victoria GFP, we developed dual FRET-based caspase-3 biosensors. Owing to their distinct excitation profiles, each FRET biosensor can be ratiometrically imaged in the presence of the other.
Ai
Hui-wang
HW
University of Alberta, Department of Chemistry, 11227 Saskatchewan Drive, Edmonton, Alberta T6G 2G2, Canada.
Hazelwood
Kristin L
KL
Davidson
Michael W
MW
Campbell
Robert E
RE
D000595:Amino Acid Sequence; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D053148:Caspase 3 / Q000378:metabolism; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D049452:Green Fluorescent Proteins; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008164:Luminescent Proteins; D010940:Plant Proteins / Q000737:chemistry
D008164:Luminescent Proteins; D010940:Plant Proteins; D049452:Green Fluorescent Proteins; D053148:Caspase 3