Mesenchymal stem cells and platelet-rich plasma enhance bone formation in sinus grafting: a histomorphometric study in minipigs.
OBJECTIVE
Autologous, allogenic, and alloplastic materials for sinus augmentation have specific drawbacks, which has stimulated an ongoing search for new materials and tissue-engineering constructs. We investigated whether mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) seeded on a fluorohydroxyapatite (FH) scaffold can improve bone formation and bone-to-implant contact (BIC) in maxillary sinus grafting.


METHODS
Bilateral sinus augmentation procedures were performed in eight minipigs. MSCs, PRP, and FH scaffold (test site) or FH alone (control site) were grafted in each maxillary sinus. Distal to the osteotomy, one dental implant per sinus was placed in the grafting material through the facial sinus wall. The animals were killed 3 months after grafting, and block sections of the implant sites were harvested and prepared for histomorphometric analysis.


RESULTS
After 12 weeks, a significant increase in bone formation occurred in the test sites compared with the control sites (42.51%versus 18.98%; p=0.001). In addition, BIC was significantly greater in the test sites compared with the control sites in the regenerated area (23.71%versus 6.63%; p=0.028).


CONCLUSIONS
These findings show that sinus augmentation with MSCs-PRP, combined with FH may enhance bone formation and osseointegration of dental implants compared with FH alone in minipigs.
Pieri
Francesco
F
Department of Odontostomatological Sciences, University of Bologna, Bologna, Italy. checcopieri@yahoo.it
Lucarelli
Enrico
E
Corinaldesi
Giuseppe
G
Iezzi
Giovanna
G
Piattelli
Adriano
A
Giardino
Roberto
R
Bassi
Massimo
M
Donati
Davide
D
Marchetti
Claudio
C
D000818:Animals; D001861:Bone Regeneration; D016025:Bone Transplantation; D003758:Dental Implantation, Endosseous; D006882:Hydroxyapatites; D008443:Maxillary Sinus / Q000601:surgery*; D045164:Mesenchymal Stem Cell Transplantation; D013516:Oral Surgical Procedures, Preprosthetic; D016348:Osseointegration; D053657:Platelet-Rich Plasma; D013552:Swine; D013556:Swine, Miniature; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds
D006882:Hydroxyapatites; C116586:fluorohydroxyapatite