title: Purification and concentration of DNA from aqueous solutions.
abstract: This unit presents basic procedures for manipulating solutions of single- or double-stranded DNA through purification and concentration steps. Phenol extraction with ethanol precipitation is described along with an alternate procedure of precipitating DNA with isopropanol. A Support Protocol describes concentration of DNA using butanol.
authors.affiliation: Baylor College of Medicine, Houston, Texas, USA.
authors.forename: D
authors.initials: D
authors.lastname: Moore
mesh_terms: D020001:1-Butanol; D019840:2-Propanol; D011232:Chemical Precipitation; D004247:DNA / Q000302:isolation & purification*; D000431:Ethanol; D008967:Molecular Biology / Q000379:methods*; D010636:Phenols; D012996:Solutions
chemical_list: D010636:Phenols; D012996:Solutions; D000431:Ethanol; D020001:1-Butanol; D004247:DNA; D019840:2-Propanol