Expressed sequence tag analysis of Antarctic hairgrass Deschampsia antarctica from King George Island, Antarctica.
Deschampsia antarctica is the only monocot that thrives in the tough conditions of the Antarctic region. It is an invaluable resource for the identification of genes associated with tolerance to various environmental pressures. In order to identify genes that are differentially regulated between greenhouse-grown and Antarctic field-grown plants, we initiated a detailed gene expression analysis. Antarctic plants were collected and greenhouse plants served as controls. Two different cDNA libraries were constructed with these plants. A total of 2,112 cDNA clones was sequenced and grouped into 1,199 unigene clusters consisting of 243 consensus and 956 singleton sequences. Using similarity searches against several public databases, we constructed a functional classification of the ESTs into categories such as genes related to responses to stimuli, as well as photosynthesis and metabolism. Real-time PCR analysis of various stress responsive genes revealed different patterns of regulation in the different environments, suggesting that these genes are involved in responses to specific environmental factors.
Lee
Hyoungseok
H
Polar BioCenter, Korea Polar Research Institute (KOPRI), Korea Ocean Research and Development Institute (KORDI), Incheon 406-840, Korea.
Cho
Hyun Hee
HH
Kim
Il-Chan
IC
Yim
Joung Han
JH
Lee
Hong Kum
HK
Lee
Yoo Kyung
YK
D000864:Antarctic Regions; D016000:Cluster Analysis; D030541:Databases, Genetic; D017753:Ecosystem; D020224:Expressed Sequence Tags; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005843:Geography; D006109:Poaceae / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D012333:RNA, Messenger