Development of FET-type albumin sensor for diagnosing nephritis.
An albumin biosensor based on a potentiometric measurement using Biofield-effect-transistor (BioFET) has been designed and fabricated, and its characteristics were investigated. The BioFET was fabricated using semiconductor integrated circuit (IC) technology. The gate surface of the BioFET was chemically modified by newly developed self-assembled monolayer (SAM) synthesized by a thiazole benzo crown ether ethylamine (TBCEA)-thioctic acid to immobilize anti-albumin. SAM formation, antibody immobilization, and antigen-antibody interaction were verified using surface plasmon resonance (SPR). The output voltage changes of the BioFET with respect to various albumin concentrations were obtained. Quasi-reference electrode (QRE) and reference FET (ReFET) has been integrated with the BioFET, and its output characteristic was investigated. The results demonstrate the feasibility of the BioFET as the albumin sensor for diagnosing nephritis.
Park
Keun-Yong
KY
School of Electrical Engineering & Computer Science, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea.
Sohn
Young-Soo
YS
Kim
Chang-Kyu
CK
Kim
Hong-Seok
HS
Bae
Young-Seuk
YS
Choi
Sie-Young
SY
D000418:Albumins / Q000032:analysis*; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D009393:Nephritis / Q000097:blood* / Q000175:diagnosis*; D014173:Transistors, Electronic
D000418:Albumins; D015415:Biomarkers