title: Permethylation linkage analysis techniques for residual carbohydrates.
abstract: Permethylation analysis is the classic approach to establishing the position of glycosidic linkages between sugar residues. Typically, the carbohydrate is derivatized to form acid-stable methyl ethers, hydrolyzed, peracetylated, and analyzed by gas chromatography-mass spectrometry. The position of glycosidic linkages in the starting carbohydrate are apparent from the mass spectra as determined by the location of acetyl residues. The completeness of permethylation is dependent upon the choice of base catalyst and is readily confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry mass spectrometry. For the permethylation of beta-cyclodextrin, Hakomori dimsyl base is shown to be superior to the NaOH-dimethyl sulfoxide system, and the use of the latter resulted in selective under-methylation of the 3-hydroxy groups. These techniques are highly applicable to residual carbohydrates from biofuel processes.
authors.affiliation: USDA-ARS-NCAUR, Bioproducts & Biocatalysis Research Unit, 1815 North University Street, Peoria, IL 61604, USA, neil.price@ars.usda.gov
authors.forename: Neil P J
authors.initials: NP
authors.lastname: Price
mesh_terms: D000465:Algorithms; D002241:Carbohydrates / Q000737:chemistry*; D003198:Computer Simulation; D003432:Cross-Linking Reagents; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006027:Glycosides / Q000737:chemistry*; D008745:Methylation; D008956:Models, Chemical
chemical_list: D002241:Carbohydrates; D003432:Cross-Linking Reagents; D006027:Glycosides