Preparation of 2'-deoxy-2'-methylseleno-modified phosphoramidites and RNA.
The derivatization of nucleic acids with selenium is a useful approach to facilitate phase determination during three-dimensional structural analysis by X-ray crystallography. This unit describes (1) the synthesis and characterization of 2'-deoxy-2'-methylseleno (2'-Se-methyl) nucleosides and their corresponding 3'-O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite derivatives, (2) the site-specific incorporation of 2'-Se-methyl ribonucleosides into oligoribonucleotides by chemical RNA solid-phase synthesis, and (3) the enzymatic ligation of Se-containing RNA oligonucleotides to produce a biologically relevant RNA sequence.
Micura
Ronald
R
Leopold-Franzens University, Innsbruck, Austria.
Höbartner
Claudia
C
Rieder
Renate
R
Kreutz
Christoph
C
Puffer
Barbara
B
Lang
Kathrin
K
Moroder
Holger
H
D000577:Amides / Q000138:chemical synthesis* / Q000737:chemistry; D018360:Crystallography, X-Ray; D009682:Magnetic Resonance Spectroscopy; D009690:Nucleic Acid Conformation; D016566:Organoselenium Compounds / Q000737:chemistry*; D010756:Phosphoric Acids / Q000138:chemical synthesis* / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
D000577:Amides; D016566:Organoselenium Compounds; D010756:Phosphoric Acids; D012313:RNA; C008493:methylselenic acid; C011067:phosphoramidic acid