Analysis of protein acylation.
Protein acylation is the covalent attachment of fatty acids to a protein; the most commonly added fatty acids are myristate (14:0) and palmitate (16:0). In this unit, protocols describe the use of radiolabeled fatty acids to label eukaryotic cells in vitro. The radiolabeled material produced can then be analyzed by the various methods described here: determination of the type of fatty acid linkage, checking for interconversion by determining the nature of the protein-bound label, and identification of the protein-bound fatty acid.
Jackson
C S
CS
National Institute for Medical Research, London, United Kingdom.
Magee
A I
AI
D000215:Acylation; D001671:Biochemistry / Q000379:methods*; D002457:Cell Extracts; D009227:Myristic Acids / Q000737:chemistry / Q000378:metabolism; D019308:Palmitic Acid / Q000737:chemistry / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D017475:Receptors, Nerve Growth Factor / Q000032:analysis; D013194:Staining and Labeling
D002457:Cell Extracts; D009227:Myristic Acids; D011506:Proteins; D017475:Receptors, Nerve Growth Factor; D019308:Palmitic Acid