Strategies for induction of protective immunity to bovine herpesvirus-1 in newborn calves with maternal antibodies.
The objective of this study was to evaluate Th1 promoting strategies for vaccination of neonates against bovine herpesvirus-1 (BHV-1). A plasmid encoding a secreted truncated version of glycoprotein D (tgD) and tgD protein formulated with CpG oligodeoxynucleotide (ODN) effectively primed the immune system of newborn lambs, whereas without CpG ODN the tgD protein was less effective. Furthermore, a heterologous DNA prime-protein/CpG boost induced stronger and more balanced immune responses than either the DNA vaccine or a protein/CpG prime-DNA boost. Three of these strategies were compared as an approach to induce protective immunity in newborn calves with BHV-1-specific maternal antibodies. Whereas the DNA vaccine induced minimal protection, the DNA prime-protein boost resulted in reduced temperature response, weight loss and virus shedding in comparison to the placebo group. Close to complete protection against BHV-1 challenge was elicited in the calves immunized with the protein/CpG formulation, as these animals lost very little weight, had only slightly elevated temperatures and shed almost no virus.
van Drunen Littel-van den Hurk
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Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3. sylvia.vandenhurk@usask.ca
Snider
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Thompson
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Latimer
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Babiuk
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D000818:Animals; D000831:Animals, Newborn; D000914:Antibodies, Viral / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology / Q000517:prevention & control; D004279:DNA, Viral / Q000276:immunology; D005260:Female; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000662:veterinary; D007242:Herpesvirus 1, Bovine / Q000276:immunology*; D022283:Herpesvirus Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008431:Maternal-Fetal Exchange; D009838:Oligodeoxyribonucleotides / Q000008:administration & dosage / Q000276:immunology; D011247:Pregnancy; D014764:Viral Proteins / Q000008:administration & dosage / Q000276:immunology
D000914:Antibodies, Viral; C408982:CPG-oligonucleotide; D004279:DNA, Viral; D022283:Herpesvirus Vaccines; D009838:Oligodeoxyribonucleotides; D014764:Viral Proteins; C046998:bovine herpesvirus type-1 glycoproteins