title: Interaction of enterohemorrhagic Escherichia coli O157:H7 with mouse intestinal mucosa.
abstract: In this study, we used mouse ileal loops to investigate the interaction of enterohemorrhagic Escherichia coli (EHEC) O157:H7 with the mouse intestinal mucosa. With a dose of 10(9) and 3 h incubation, EHEC O157 was detected in the lumen and to a lesser extent associated with the epithelium. Typical attaching and effacing (A/E) lesions were seen, albeit infrequently. While the effector protein Tir was essential for A/E lesion formation, the bacterial type III secretion system adaptor protein TccP was dispensable. These results suggest that A/E lesions on mouse intestinal mucosa can be formed independently of robust actin polymerization.
authors.affiliation: Divison of Cell & Molecular Biology, Imperial College London, London, UK. f.girard@imperial.ac.uk
authors.forename: Francis
authors.initials: F
authors.lastname: Girard
authors.forename: Gad
authors.initials: G
authors.lastname: Frankel
authors.forename: Alan D
authors.initials: AD
authors.lastname: Phillips
authors.forename: William
authors.initials: W
authors.lastname: Cooley
authors.forename: Ute
authors.initials: U
authors.lastname: Weyer
authors.forename: Alexandra H A
authors.initials: AH
authors.lastname: Dugdale
authors.forename: Martin J
authors.initials: MJ
authors.lastname: Woodward
authors.forename: Roberto M
authors.initials: RM
authors.lastname: La Ragione
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion; D019453:Escherichia coli O157 / Q000502:physiology*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D007082:Ileum / Q000382:microbiology; D007413:Intestinal Mucosa / Q000382:microbiology*; D051379:Mice; D008813:Mice, Inbred ICR; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; D011956:Receptors, Cell Surface; C109287:Tir protein, E coli; C505370:tccP protein, E coli