Selective phospholipid methanolysis catalyzed by a weakly acidic microbial polysaccharide, zooglan.
Zooglan, a weakly acidic exopolysaccharide produced by Zoogloea ramigera 115, catalyzed the preferential methanolysis of phospatidylcholine compared to other phospholipids when the reaction was carried out in pure methanol at 30 degrees C. The reaction was monitored by thin-layer chromatography (TLC) as well as (1)H and (31)P nuclear magnetic resonance (NMR) spectrometry. Zooglan enhanced the rate of methanolysis of dipalmitoylphosphatidylcholine (DPPC) up to about 170-fold compared to controls such as DPPC alone, pyruvic acid, succinic acid and acetic acid. Furthermore, the methanolysis was different depending on the head groups of the phospholipids. Through this study, we have shown that zooglan can act as an environmentally benign catalytic polysaccharide for methanolysis in pure methanol solution.
Lee
Sanghoo
S
Department of Bioscience and Biotechnology, Bio/Molecular Informatics Center, Konkuk University, Gwangjin-Gu, Seoul, Republic of Korea.
Jung
Seunho
S
D015060:1,2-Dipalmitoylphosphatidylcholine / Q000737:chemistry; D002384:Catalysis; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D015045:Zoogloea / Q000737:chemistry
D010743:Phospholipids; D011135:Polysaccharides, Bacterial; D015060:1,2-Dipalmitoylphosphatidylcholine; D000432:Methanol