[Headspace solid-phase microextraction-gas chromatography-mass spectrometry for analysis of volatile components from pollen pini].
Headspace solid-phase microextraction HS-SPME) technique was employed to extract the volatile compounds from Pollen Pini. The volatile components were isolated and identified successfully by gas chromatography-mass spectrometry (GC-MS). The volatiles were collected using several SPME parameters (including extraction time and temperature). The best response was obtained using a PA fiber by when the extraction temperature was 70 degrees C, extraction time was 30 minutes and desorption time was 10 time. Analysis was performed by GC-MS. Seventy components were identified by HS-SPME. The main components were fatty compounds. The results showed that the HS-SPME technique could be used to extract the volatile components from HS-SPME-GC-MS.
Zhang
Xiao-shan
XS
Center of Analysis and Testing of Shantou University, Shantou 515063, China. zhangxs@stu.edu.cn
Chen
Tu-feng
TF
Zhang
Hai-dan
HD
Gao
Wen-hua
WH
D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D005223:Fats / Q000032:analysis / Q000302:isolation & purification; D005232:Fatty Acids, Volatile / Q000032:analysis / Q000302:isolation & purification; D035264:Flowers; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000737:chemistry / Q000302:isolation & purification*; D019308:Palmitic Acid / Q000032:analysis / Q000302:isolation & purification; D028223:Pinus / Q000737:chemistry*; D011058:Pollen / Q000737:chemistry; D013729:Terpenes / Q000032:analysis / Q000302:isolation & purification*
D004365:Drugs, Chinese Herbal; D005223:Fats; D005232:Fatty Acids, Volatile; D009822:Oils, Volatile; D013729:Terpenes; D019308:Palmitic Acid