Selection for catalytic function with nucleic acids.
For in vitro selection of catalytic polynucleotides, each new protocol must be designed to harness the desired catalytic activity to help propel the selection process itself. This unit gives guidelines for design of in vitro selection experiments for catalytic function. It outlines several representative protocols as examples of successful selection experiments, providing a conceptual basis for the design and implementation of new selective-amplification protocols for nucleic acids.
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Yale University, New Haven, Connecticut, USA.
D001483:Base Sequence; D002384:Catalysis; D008025:Ligases / Q000378:metabolism; D016296:Mutagenesis; D009696:Nucleic Acids / Q000302:isolation & purification* / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D016337:RNA, Catalytic / Q000378:metabolism; D043262:Ribonuclease P / Q000378:metabolism; D052156:SELEX Aptamer Technique / Q000379:methods*
D009696:Nucleic Acids; D016337:RNA, Catalytic; D043262:Ribonuclease P; D008025:Ligases