Prostaglandin E1 transport into rat intestinal cryptic cell line IEC-6.
Administration of antitumor drugs such as 5-fluorouracil and methotrexate to rats causes intestinal injury. The intestinal injury was reported to be prevented by coadministration of prostaglandin E1 analogues to rats. IEC-6 cell is a useful in vitro model to study the cytoprotective effect in enterocytes, For its purpose, it is important to know whether or not prostaglandins are taken up into IEC-6 cells. The transport of prostaglandin E1 into IEC-6 cells was investigated, using [3H]-prostaglandin E1. Prostaglandin E1 was found to be transported into IEC-6 cells by the passive diffusion mechanism.
Hirata
Kohji
K
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences Chiba University, 1-8-1 Inohana, Chuoku-ku, Chiba 260-8675, Japan.
Mabashi
Kumiko
K
Horie
Toshiharu
T
D019297:2,4-Dinitrophenol / Q000494:pharmacology; D000527:Alprostadil / Q000378:metabolism*; D000818:Animals; D001693:Biological Transport, Active; D001961:Bromcresol Green; D002352:Carrier Proteins / Q000378:metabolism; D002460:Cell Line; D004396:Coloring Agents; D015194:Diffusion Chambers, Culture; D020895:Enterocytes / Q000187:drug effects / Q000378:metabolism*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology / Q000187:drug effects; D007700:Kinetics; D051381:Rats; D014475:Uncoupling Agents / Q000494:pharmacology
D002352:Carrier Proteins; D004396:Coloring Agents; D014475:Uncoupling Agents; D001961:Bromcresol Green; D000527:Alprostadil; D019297:2,4-Dinitrophenol