title: Identification of toxic oligopeptides in Amanita fungi employing capillary electrophoresis-electrospray ionization-mass spectrometry with positive and negative ion detection.
abstract: The identification of toxic oligopeptides employing CE-ESI-MS is presented. The analytes studied ama- and phallotoxins are of significant forensic interest because over 90% of the lethal cases of fungus poisoning in man are caused by species of Amanita which contain these toxins. A CE method was developed to separate the toxins alpha-, beta- and gamma-amanitin, phalloidin and phallacidin. Their fragmentation patterns in MS(n) experiments were investigated in the positive and in the negative ion mode, also the influence of the sheath liquid mixture of the used interface on the S/N. Method validation included the determination of the LOD and the repeatability of the migration time and peak area for both detection modes. With the optimized method LODs of 13-79 ng/mL (17-87 nmol/L) were reached. The CE-MS procedure was successfully applied to the identification of ama- and phallotoxins in extracts of air-dried mushroom samples.
authors.affiliation: University of Marburg, Department of Chemistry, Marburg, Germany. rittgen@web.de
authors.forename: Jan
authors.initials: J
authors.lastname: Rittgen
authors.forename: Michael
authors.initials: M
authors.lastname: Pütz
authors.forename: Ute
authors.initials: U
authors.lastname: Pyell
mesh_terms: D000545:Amanita / Q000737:chemistry* / Q000472:pathogenicity; D000546:Amanitins / Q000032:analysis / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D015394:Molecular Structure; D009145:Mushroom Poisoning; D009183:Mycotoxins / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D009842:Oligopeptides / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D010456:Peptides, Cyclic / Q000032:analysis / Q000737:chemistry; D010590:Phalloidine / Q000032:analysis / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000546:Amanitins; D009183:Mycotoxins; D009842:Oligopeptides; D010456:Peptides, Cyclic; D010590:Phalloidine; C017613:phallacidin