title: An oil-emulsion vaccine induces full-protection against Mycoplasma agalactiae infection in sheep.
abstract: The immunogenicity and efficacy of three inactivated vaccines (A, B, C) prepared with Mycoplasma agalactiae (M. agalactiae) and with different oil-emulsion adjuvants were evaluated in sheep. Twenty-eight animals were used, divided into four groups (a, b, c, d) of seven animals each. Three groups were immunized with the same vaccine, but using different adjuvants, while one group (d) was used as an unvaccinated control group. All the vaccine formulations were able to induce clinical protection of animals after challenge with M. agalactiae, but only vaccine C, emulsioned with Montanide ISA-563, Marcol-52 and Montane-80 (ratio: 30%, 63%, 7% respectively), was able to induce full protection in challenged animals, preventing both the onset of clinical signs and infection by M. agalactiae.
authors.affiliation: Department of Animal Health and Well-being, Faculty of Veterinary Medicine, University of Bari, Italy. d.buonavoglia@veterinaria.uniba.it
authors.forename: Domenico
authors.initials: D
authors.lastname: Buonavoglia
authors.forename: Grazia
authors.initials: G
authors.lastname: Greco
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Quaranta
authors.forename: Marialaura
authors.initials: M
authors.lastname: Corrente
authors.forename: Vito
authors.initials: V
authors.lastname: Martella
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology / Q000627:therapeutic use; D004655:Emulsions / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D008899:Mineral Oil / Q000008:administration & dosage / Q000009:adverse effects / Q000627:therapeutic use; D009175:Mycoplasma Infections / Q000382:microbiology / Q000517:prevention & control / Q000662:veterinary*; D045644:Mycoplasma agalactiae / Q000276:immunology*; D012756:Sheep; D012757:Sheep Diseases / Q000382:microbiology / Q000517:prevention & control*; D016896:Treatment Outcome; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology / Q000627:therapeutic use
chemical_list: D000276:Adjuvants, Immunologic; D000907:Antibodies, Bacterial; D001428:Bacterial Vaccines; D004655:Emulsions; D015164:Vaccines, Inactivated; D008899:Mineral Oil