[Expression of human ermap gene in umbilical cord blood mononuclear cells during differentiation and development towards erythroid lineage].
The aim of study was to explore the potential of human erythroid membrane associated protein (ERMAP) gene in erythroid cell differentiation and development, mononuclear cells (MNCs) were isolated from umbilical cord blood and induced to erythroid cell differentiation by SCF, IL-3 and EPO. The cell morphology was observed by using optical microscopy, the positive rate of cells was counted by biphenylamine staining and the ratios of CD36+/CD235a-, CD36+/CD235a+, CD36-/CD235a+ cells were detected by flow cytometry, the change of human ermap gene expression level was analyzed by using fluorescent quantitative PCR (FQ-PCR). The results showed that the ermap gene expression level increased while MNCs were induced to erythroid lineage after treatment with SCF, IL-3 and EPO. It is concluded that the human ermap gene plays an important role in differentiation and development of erythroid cells.
Lin
Li-Dan
LD
Department of Pediatrics, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, Guangdong Province, China.
He
Xin-Rong
XR
Ye
Tie-Zhen
TZ
He
Ying-Yi
YY
Guan
Jing-Ming
JM
Chen
Ying
Y
Liang
Jie-Fang
JF
D001789:Blood Group Antigens / Q000235:genetics / Q000378:metabolism*; D000071797:Butyrophilins; D002454:Cell Differentiation / Q000235:genetics*; D002478:Cells, Cultured; D041905:Erythroid Cells / Q000166:cytology*; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods
D001789:Blood Group Antigens; D000071797:Butyrophilins; C472715:scianna antigens