Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures.
To develop an optimal bioprocess for secondary metabolite production and explain the bioprocess at the molecular level, we examine the synergistic effects of sequential treatment with methyl jasmonate (MJ), salicylic acid (SA) and yeast extract (YE) on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures. Serial treatment of MJ, SA and YE at 24h intervals enhanced the accumulation of dihydrosanguinarine (2.5 times) and sanguinarine (5.5 times). This sequential treatment using different signal elicitors was more effective than single elicitor or simultaneous treatment of the elicitors; it induced benzophenanthridine alkaloid accumulation to 917.7+/-42.0mg/L. Also, (S)-methylcoclaurine-3'-hydroxylase (CYP80B1) and 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase (4'OMT) expressions among enzymes in sanguinarine biosynthetic pathway explained the synergistic effects by sequential treatment of the elicitors. The sequential treatment strategy using elicitors related to different signal transduction pathways can be used to design better processes to increase accumulation of secondary metabolites in plant cell culture. Analysis of protein expression provides the detailed information about metabolite accumulation through the correlated results.
Cho
Hwa-Young
HY
Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang 790-784, South Korea.
Son
Seok Young
SY
Rhee
Hong Soon
HS
Yoon
Sung-Yong H
SY
Lee-Parsons
Carolyn W T
CW
Park
Jong Moon
JM
D000085:Acetates / Q000008:administration & dosage*; D000470:Alkaloids / Q000378:metabolism; D053119:Benzophenanthridines / Q000378:metabolism*; D002457:Cell Extracts / Q000008:administration & dosage* / Q000737:chemistry; D002478:Cells, Cultured; D003517:Cyclopentanes / Q000008:administration & dosage*; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004357:Drug Synergism; D031675:Eschscholzia / Q000187:drug effects / Q000378:metabolism*; D054883:Oxylipins / Q000008:administration & dosage*; D010940:Plant Proteins / Q000378:metabolism*; D020156:Salicylic Acid / Q000008:administration & dosage*; D015003:Yeasts / Q000737:chemistry*
D000085:Acetates; D000470:Alkaloids; D053119:Benzophenanthridines; D002457:Cell Extracts; D003517:Cyclopentanes; D004338:Drug Combinations; D054883:Oxylipins; D010940:Plant Proteins; C072239:methyl jasmonate; D020156:Salicylic Acid