title: Proteomic profiling and identification in peritoneal fluid of children treated by peritoneal dialysis.
abstract: BACKGROUND
  Proteomic technologies offer a high-throughput analysis of the expression of proteins in biological samples. The global analysis of the proteins in peritoneal dialysis (PD) fluid will provide a better understanding of the biological processes of the peritoneal membrane.
  
  
  METHODS
  The dialysate of nine paediatric PD patients was collected from peritoneal equilibrium tests with 3.86% glucose. Proteins were separated on a 10% SDS-PAGE gel and in-gel digested with trypsin. Peptide mixtures were analysed using nanoLC-MS/MS and results were searched against the NCBI database.
  
  
  RESULTS
  A total number of 189 proteins were identified in the PD fluid of nine patients, with 88 proteins shared by all patients. These 88 proteins accounted for 47% of the identified proteins and >90% of the total protein content in the analysed samples. Proteins were subdivided into eight different classes according to function.
  
  
  CONCLUSIONS
  This study gives a representative overview of the proteins present in PD fluid. The proteins in PD fluid reflect plasma proteins as well as local peritoneal processes. Potentially interesting proteins are revealed.
authors.affiliation: Department of Paediatric Nephrology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands. r.raaijmakers@cukz.umcn.nl
authors.forename: Renske
authors.initials: R
authors.lastname: Raaijmakers
authors.forename: Wendy
authors.initials: W
authors.lastname: Pluk
authors.forename: Cornelis H
authors.initials: CH
authors.lastname: Schröder
authors.forename: Jolein
authors.initials: J
authors.lastname: Gloerich
authors.forename: Elisabeth A M
authors.initials: EA
authors.lastname: Cornelissen
authors.forename: Hans J C T
authors.initials: HJ
authors.lastname: Wessels
authors.forename: Johannes L
authors.initials: JL
authors.lastname: Willems
authors.forename: Leo A H
authors.initials: LA
authors.lastname: Monnens
authors.forename: Lambert P W J
authors.initials: LP
authors.lastname: van den Heuvel
mesh_terms: D001202:Ascitic Fluid / Q000737:chemistry*; D002648:Child; D002675:Child, Preschool; D002853:Chromatography, Liquid; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D010530:Peritoneal Dialysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome