Response surface optimization of the critical medium components for pullulan production by Aureobasidium pullulans FB-1.
Culture conditions for pullulan production by Aureobasidium pullulans were optimized using response surface methodology at shake flask level without pH control. In the present investigation, a five-level with five-factor central composite rotatable design of experiments was employed to optimize the levels of five factors significantly affecting the pullulan production, biomass production, and sugar utilization in submerged cultivation. The selected factors included concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride. Using this methodology, the optimal values for concentration of sucrose, ammonium sulphate, yeast extract, dipotassium hydrogen phosphate, and sodium chloride were 5.31%, 0.11%, 0.07%, 0.05%, and 0.15% (w/v), respectively. This optimized medium has projected a theoretically production of pullulan of 4.44%, biomass yield of 1.03%, and sugar utilization of 97.12%. The multiple correlation coefficient 'R' was 0.9976, 0.9761 and 0.9919 for pullulan production, biomass production, and sugar utilization, respectively. The value of R being very close to one justifies an excellent correlation between the predicted and the experimental data.
Singh
Ram Sarup
RS
Carbohydrate and Protein Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala 147 002, Punjab, India. rssingh11@lycos.com
Singh
Harpreet
H
Saini
Gaganpreet Kaur
GK
D000645:Ammonium Sulfate / Q000494:pharmacology; D001203:Ascomycota / Q000187:drug effects* / Q000378:metabolism*; D018533:Biomass; D003470:Culture Media / Q000737:chemistry* / Q000494:pharmacology*; D005936:Glucans / Q000096:biosynthesis*; D008954:Models, Biological; D010710:Phosphates / Q000494:pharmacology; D017680:Potassium Compounds / Q000494:pharmacology; D012965:Sodium Chloride / Q000494:pharmacology; D013395:Sucrose / Q000494:pharmacology; D015003:Yeasts / Q000737:chemistry
D003470:Culture Media; D005936:Glucans; D010710:Phosphates; D017680:Potassium Compounds; D012965:Sodium Chloride; D013395:Sucrose; C009109:pullulan; C013216:potassium phosphate; D000645:Ammonium Sulfate