title: Assaying proteases in cellular environments.
abstract: To better study protease activation, regulation, and inhibition physiologically or pharmacologically, it is often advantageous to use cell-based systems. The introduction of the cellular environment poses additional challenges for the assay of the protease of interest. In this unit, various methods for monitoring protease activity in cell-based systems is discussed. The scope of the unit is restricted to mammalian cell system. It is also stressed that specific considerations or requirements for a particular protease or peptidase are often needed in order to optimize the assay.
authors.affiliation: Pfizer Global Research and Development, Ann Arbor, Michigan, USA.
authors.forename: Kevin K W
authors.initials: KK
authors.lastname: Wang
mesh_terms: D000818:Animals; D002477:Cells / Q000201:enzymology; D004789:Enzyme Activation; D006801:Humans; D008722:Methods; D010447:Peptide Hydrolases / Q000032:analysis* / Q000378:metabolism*; D011480:Protease Inhibitors
chemical_list: D011480:Protease Inhibitors; D010447:Peptide Hydrolases