title: Damage of embryo development caused by peroxidized mineral oil and its association with albumin in culture.
abstract: OBJECTIVE
  To examine the effect of free radicals from peroxidized oil and the role of albumin on the passage of radicals.
  
  
  METHODS
  Prospective study.
  
  
  METHODS
  Clinical IVF laboratory and university department.
  
  
  METHODS
  Blood samples were donated by laboratory staff.
  
  
  METHODS
  Examination of the effects of mineral oil samples with various peroxide value (POV) on culture of erythrocytes and on the passage of a lipophilic tracer, DiI, into the zona pellucida.
  
  
  METHODS
  Time required for hemolysis of red blood cells by peroxidized oil, staining of zona pellucida from human oocytes and embryos by lipophilic tracer, and POV analysis of mineral oil samples in relation to various storage conditions.
  
  
  RESULTS
  The time for hemolysis was related to the POV levels of oil samples covering the culture medium. Albumin in the medium facilitated hemolysis and the passage of DiI into the zona. Peroxidized oil (POV >0.02 meq/kg) blocked the entry of DiI into the zona.
  
  
  CONCLUSIONS
  The presence of albumin in the medium was associated with the entry into the human zona of agents present in peroxidized mineral oil. This process and variable oil peroxidation could be deleterious to embryos in culture.
authors.affiliation: Nagai Clinic, Saitama, Japan. otsuki.midori.junko@gmail.com
authors.forename: Junko
authors.initials: J
authors.lastname: Otsuki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Nagai
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Chiba
mesh_terms: D002478:Cells, Cultured; D003470:Culture Media; D047108:Embryonic Development / Q000187:drug effects*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects; D005260:Female; D005609:Free Radicals; D006801:Humans; D008899:Mineral Oil / Q000737:chemistry / Q000633:toxicity*; D012709:Serum Albumin / Q000494:pharmacology*; D015044:Zona Pellucida / Q000378:metabolism
chemical_list: D003470:Culture Media; D005609:Free Radicals; D012709:Serum Albumin; D008899:Mineral Oil