title: The tick salivary protein Salp15 inhibits the killing of serum-sensitive Borrelia burgdorferi sensu lato isolates.
abstract: Borrelia burgdorferi, the agent of Lyme disease, is transmitted by ticks. During transmission from the tick to the host, spirochetes are delivered with tick saliva, which contains the salivary protein Salp15. Salp15 has been shown to protect spirochetes against B. burgdorferi-specific antibodies. We now show that Salp15 from both Ixodes ricinus and Ixodes scapularis protects serum-sensitive isolates of Borrelia burgdorferi sensu lato against complement-mediated killing. I. ricinus Salp15 showed strong protective effects compared to those of I. scapularis Salp15. Deposition of terminal C5b to C9 (one molecule each of C5b, C6, C7, and C8 and one or more molecules of C9) complement complexes, part of the membrane attack complex, on the surface of B. burgdorferi was inhibited in the presence of Salp15. In the presence of normal human serum, serum-sensitive Borrelia burgdorferi requires protection against complement-mediated killing, which is provided, at least in part, by the binding to the tick salivary protein Salp15.
authors.affiliation: Leiden University Medical Centre, Department of Medical Microbiology, University of Leiden, Albinusdreef 2, 2333 ZA Leiden, The Netherlands. T.J.Schuijt@lumc.nl
authors.forename: Tim J
authors.initials: TJ
authors.lastname: Schuijt
authors.forename: Joppe W R
authors.initials: JW
authors.lastname: Hovius
authors.forename: Nathalie D
authors.initials: ND
authors.lastname: van Burgel
authors.forename: Nandhini
authors.initials: N
authors.lastname: Ramamoorthi
authors.forename: Erol
authors.initials: E
authors.lastname: Fikrig
authors.forename: Alje P
authors.initials: AP
authors.lastname: van Dam
mesh_terms: D000818:Animals; D001770:Blood Bactericidal Activity; D015748:Borrelia burgdorferi Group / Q000187:drug effects* / Q000254:growth & development* / Q000276:immunology / Q000378:metabolism; D002462:Cell Membrane / Q000378:metabolism; D003167:Complement Activation / Q000276:immunology; D015938:Complement Membrane Attack Complex / Q000037:antagonists & inhibitors / Q000378:metabolism; D006801:Humans; D018884:Ixodes / Q000378:metabolism* / Q000382:microbiology; D012471:Salivary Proteins and Peptides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D015938:Complement Membrane Attack Complex; D012471:Salivary Proteins and Peptides; C464566:Salp15 protein, Ixodes scapularis