Neural stem cell transplantation in mouse brain.
Neural stem cells (NSCs) are the most primordial, least committed cells of the nervous system, and transplantation of these multipotent cells holds the promise of regenerative therapy for many central nervous system (CNS) diseases. This unit describes methods for NSC transplantation into neonatal mouse pups, embryonic mouse brain, and adult mouse brain. A description of options for detection of labeled donor cells in engrafted mouse brain is provided along with an example protocol for detecting lacZ-expressing cells in situ. Also included is a protocol for preparing NSCs for transplantation.
Lee
Jean-Pyo
JP
Stem Cell and Regeneration Program, Center for Neuroscience and Aging Research, Burnham Institute for Medical Research, La Jolla, California, USA.
McKercher
Scott
S
Muller
Franz-Josef
FJ
Snyder
Evan Y
EY
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000502:physiology; D007763:Lac Operon; D051379:Mice; D039902:Multipotent Stem Cells / Q000637:transplantation*; D009416:Nerve Regeneration / Q000502:physiology*; D009474:Neurons / Q000166:cytology*; D033581:Stem Cell Transplantation / Q000379:methods*