Theory and application of fluorescence microscopy.
The growing importance in biology and especially in neurobiology of fluorescence microscopy is due to (1) the extraordinary development of new fluorescent molecular probes and (2) the development of improved low light level imaging systems and confocal microscopy techniques. This overview covers fluorescent molecular probes, filters and filter sets, multiband filters and multidye fluorescence, light sources, microscope objectives, image resolution and the point-spread function, and general steps for immunolabeling.
Coling
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University of California Berkeley, Berkeley, California, USA.
Kachar
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B
D000818:Animals; D002478:Cells, Cultured; D005456:Fluorescent Dyes; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D015335:Molecular Probes; D046509:Tissue Culture Techniques / Q000295:instrumentation / Q000379:methods
D005456:Fluorescent Dyes; D015335:Molecular Probes