title: Detection of phosphorylation by enzymatic techniques.
abstract: This unit presents protocols for examining the functional effects elicited by nonspecific acid or alkaline phosphatases that dephosphorylate many phosphoproteins in vitro. Additional protocols describe digestion of phosphoproteins with a protein serine/threonine phosphatase and protein tyrosine phosphatase. A support protocol has been included to identify the radiolabel as (32)P(I) based on its ability to form a complex with ammonium molybdate.
authors.affiliation: Duke University Medical Center, Durham, North Carolina, USA.
authors.forename: S
authors.initials: S
authors.lastname: Shenolikar
mesh_terms: D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D001671:Biochemistry / Q000379:methods*; D010749:Phosphoprotein Phosphatases / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis* / Q000378:metabolism*; D010744:Phosphoric Monoester Hydrolases / Q000378:metabolism*; D010761:Phosphorus Radioisotopes; D010766:Phosphorylation
chemical_list: D010750:Phosphoproteins; D010761:Phosphorus Radioisotopes; D000469:Alkaline Phosphatase; D010749:Phosphoprotein Phosphatases; D000135:Acid Phosphatase; D010744:Phosphoric Monoester Hydrolases