title: Digestion of DNA with restriction endonucleases.
abstract: Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. This appendix describes restriction endonucleases and their properties.
authors.affiliation: Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Bloch
mesh_terms: D001483:Base Sequence; D002021:Buffers; D004247:DNA / Q000235:genetics / Q000378:metabolism*; D004262:DNA Restriction Enzymes / Q000378:metabolism*; D008969:Molecular Sequence Data
chemical_list: D002021:Buffers; D004247:DNA; D004262:DNA Restriction Enzymes