[Comparison of content of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside and anthraquinon in different ploidy Radix Polygoni Multiflori].
OBJECTIVE
To investigate the content of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside and anthraquinon in diploid and triploid Radix Polygoni Multiflori.


METHODS
4 batches of Radix Polygoni Multiflori were collected from different districts. The content of 2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucoside and anthraquinon in these samples were determined at 320 nm and 254nm wave length by HPLC with Inertsil ODS-3 C18 pillar and acetomitrile: aqua (25:75), methanol: 0.1% phosphoric (85:15) respectively as the mobile phase.


RESULTS
The maximum content of 2,3,5,4'-tetrahydroxystilbene-2-0-beta-D-glucoside was diploid Radix Polygoni Multiflori from Deqing Guangdong. The maximum ratio of total anthraquinon was triploid Radix Polygoni Multiflori from Jinxi Guangxi reached 85%.


CONCLUSIONS
The content of anthraquinon varies greatly in the samples from the different producing areas.
Jiao
Xu-wen
XW
Department of Pharmacy, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, China.
Zhang
Xiang-nian
XN
Zhao
Xuan
X
Yan
Ping
P
Zhao
Shu-jin
SJ
D000880:Anthraquinones / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D004642:Emodin / Q000032:analysis; D005960:Glucosides / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry* / Q000254:growth & development; D011003:Ploidies; D028502:Polygonum / Q000737:chemistry* / Q000254:growth & development; D015203:Reproducibility of Results; D013267:Stilbenes / Q000032:analysis*
C489707:2',3',4',5'-tetrahydroxystilbene-2-O-beta-D-glucoside; D000880:Anthraquinones; D005960:Glucosides; D013267:Stilbenes; D004642:Emodin