Chromatographic analysis of amino and organic acids in physiological fluids to detect inborn errors of metabolism.
This unit describes methods for the preparation of samples for analysis of physiological amino acids and organic acids. Amino acids are analyzed by ion-exchange chromatography using an automated system. Organic acids are analyzed by gas-chromatography/mass spectrometry (GC-MS). Analysis of amino and organic acids is necessary to detect and monitor the treatment of many inborn errors of metabolism.
Woontner
Michael
M
University of Colorado Health Sciences Center, Aurora, Colorado, USA.
Goodman
Stephen I
SI
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D001826:Body Fluids / Q000737:chemistry* / Q000502:physiology; D002264:Carboxylic Acids / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D008661:Metabolism, Inborn Errors / Q000175:diagnosis* / Q000235:genetics* / Q000378:metabolism
D000596:Amino Acids; D002264:Carboxylic Acids