Detection and quantification of fetal DNA in maternal plasma by using LightCycler technology.
Since the demonstration of cell-free fetal DNA in maternal circulation, its aberrational quantification has been explored and demonstrated in numerous clinical situations. Moreover, several centers have begun to use detection and quantification of fetal DNA to diagnose fetal genetic status with high reliability, such as fetal RhD. One of the methods for analyzing cell-free fetal DNA is quantification by using LightCycler technology. With the advent of various fluorescent reporters and greater stability of kit's reagent, real-time quantitative polymerase chain reaction by using LightCycler technology is relatively simple and fast.
Purwosunu
Yuditiya
Y
Department of Obstetrics and Gynecology, Showa University School of Medicine, Tokyo, Japan.
Sekizawa
Akihiko
A
Okai
Takashi
T
D002474:Cell-Free System; D004247:DNA / Q000097:blood* / Q000302:isolation & purification; D015342:DNA Probes; D005260:Female; D005333:Fetus / Q000378:metabolism*; D005456:Fluorescent Dyes; D018507:Gene Expression Regulation, Developmental; D005820:Genetic Testing; D006801:Humans; D008431:Maternal-Fetal Exchange; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results
D015342:DNA Probes; D005456:Fluorescent Dyes; D011933:Reagent Kits, Diagnostic; D004247:DNA