title: [Synergistic antileukemic effect of phytoestrogens and chemotherapeutic drugs on leukemic cell lines in vitro].
abstract: Natural phytoestrogens such as the isoflavones genistein and daidzein, and the flavones quercetin exhibit anti-cancer properties. This study was purpose to investigate the anti-proliferative effect of phytoestrogens on acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) cells, and their synergistic antileukemic effect in combination with chemotherapeutic drugs. Optimal dosage of genistein, quercetin and in combination with chemicals for leukemia cells were determined by experiments. Cell viability, apoptosis induction and cell cycle arrest were detected by trypan blue staining, MTT assay, optical microscopy, flow cytometry (FCM). The schedule treatment of combination of genistein and chemicals was determined. The results showed that genistein exhibited a dose- and time-dependent inhibitory effect on cell proliferation in NB4 and HL-60 cells, induced apoptosis and cell cycle arrest in G2/M phase. Quercetin had evident inhibitory effect on the proliferation of K562 and K562/A cells. The combination of genistein and chemicals exerted a synergistic effect on cell growth inhibition. In conclusion, this study demonstrated the synergistic antileukemic effect of genistein with chemotherapeutic drugs on leukemic cells. This combination appears to be a new idea for the clinical novel treatment of leukemia.
authors.affiliation: Department of Oncology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Shen
authors.forename: Wei-Jing
authors.initials: WJ
authors.lastname: Zhang
authors.forename: Yan-Chin
authors.initials: YC
authors.lastname: Tai
authors.forename: Ching-Ho Stephen
authors.initials: CH
authors.lastname: Wong
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xie
authors.forename: Chien-Shing
authors.initials: CS
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004357:Drug Synergism; D019833:Genistein / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D007948:Leukemia, Monocytic, Acute / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D048789:Phytoestrogens / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D007529:Isoflavones; D048789:Phytoestrogens; C004742:daidzein; D011794:Quercetin; D019833:Genistein