title: Postoperative peritoneal dissemination of ovarian cancer cells is not promoted by carbon-dioxide pneumoperitoneum at low intraperitoneal pressure in a syngenic mouse laparoscopic model with controlled respiratory support: a pilot study.
abstract: OBJECTIVE
  To investigate postoperative peritoneal dissemination of ovarian cancer cells in a syngenic mouse model with and without controlled respiratory support (CRS).
  
  
  METHODS
  Randomized controlled trial (Canadian Task Force classification I).
  
  
  METHODS
  Academic facility.
  
  
  METHODS
  Sixty-four female C57BJ6 mice.
  
  
  METHODS
  Mice were randomly divided into 4 surgical groups: anesthesia alone group; 2 carbon-dioxide pneumoperitoneum groups, 1 with low (2 mm Hg) and 1 with high (8 mm Hg) intraperitoneal pressure (IPP); and finally the laparotomy group. Each of the 4 groups was then subdivided into one group with CRS and the other without. Mouse ovarian cancer cells were injected intraperitoneally just before surgery.
  
  
  RESULTS
  A laparotomy was performed to evaluate postoperative peritoneal dissemination of ovarian cancer cells on postoperative day 14. A computerized analysis system was then used to evaluate peritoneal dissemination. In the groups with CRS, the peritoneal dissemination score was significantly higher in the laparotomy and high IPP groups compared with anesthesia alone (p <.0001 vs laparotomy, p <.002 vs high IPP) and low IPP (p <.0002 vs laparotomy, p <.004 vs high IPP) groups. No significant difference was detected between the low IPP and anesthesia alone groups.
  
  
  CONCLUSIONS
  Postoperative peritoneal dissemination of ovarian cancer cells is not promoted by a carbon-dioxide pneumoperitoneum with a low IPP in a mouse model with CRS when assessed on postoperative day 14.
authors.affiliation: Université d'Auvergne-Clermont I, Centre d'Endoscopie et des Nouvelles Techniques Interventionnelles, Clermont-Ferrand, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bourdel
authors.forename: Sachiko
authors.initials: S
authors.lastname: Matsuzaki
authors.forename: Jean-Etienne
authors.initials: JE
authors.lastname: Bazin
authors.forename: Claude
authors.initials: C
authors.lastname: Darcha
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Pouly
authors.forename: Gérard
authors.initials: G
authors.lastname: Mage
authors.forename: Michel
authors.initials: M
authors.lastname: Canis
mesh_terms: D000230:Adenocarcinoma / Q000556:secondary*; D000818:Animals; D002245:Carbon Dioxide; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D005260:Female; D051379:Mice; D009366:Neoplasm Seeding; D010051:Ovarian Neoplasms / Q000473:pathology*; D010534:Peritoneal Neoplasms / Q000517:prevention & control / Q000556:secondary*; D010865:Pilot Projects; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects* / Q000379:methods; D011184:Postoperative Period; D012121:Respiration, Artificial / Q000379:methods*
chemical_list: D002245:Carbon Dioxide