Protective effect of serofendic acid on ischemic injury induced by occlusion of the middle cerebral artery in rats.
We previously reported that a sulfur-containing neuroprotective substance named serofendic acid purified and isolated from fetal calf serum prevented glutamate neurotoxicity in rat cortical cultured neurons. In the present study, we investigated the effect of serofendic acid on ischemic injury induced by a transient occlusion of the middle cerebral artery in rats. Serofendic acid was intracerebroventricularly administered 30 min after the onset of the occlusion. Serofendic acid (30 nmol) significantly reduced total infarct volume, similar to edaravone (30 nmol), a free radical scavenger. Treatment with serofendic acid (1-30 nmol) reduced the infarct volume in a dose-dependent manner. Moreover, serofendic acid (30 nmol) improved neurological deficit scores. These results suggest that intracerebroventricular administration of serofendic acid prevents the neurodegeneration induced by a transient focal cerebral ischemia and reperfusion.
Nakamura
Tomohiro
T
Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan.
Kume
Toshiaki
T
Katsuki
Hiroshi
H
Niidome
Tetsuhiro
T
Sugimoto
Hachiro
H
Akaike
Akinori
A
D000818:Animals; D000983:Antipyrine / Q000031:analogs & derivatives / Q000494:pharmacology; D001835:Body Weight / Q000187:drug effects; D002545:Brain Ischemia / Q000473:pathology*; D002560:Cerebrovascular Circulation / Q000187:drug effects; D004224:Diterpenes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D000077553:Edaravone; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology / Q000517:prevention & control*; D008297:Male; D020768:Middle Cerebral Artery / Q000502:physiology; D018696:Neuroprotective Agents; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control
D004224:Diterpenes; D018696:Neuroprotective Agents; C452642:serofendic acid; D000077553:Edaravone; D000983:Antipyrine