Nucleic acid microarrays created in the double-spiral format on a circular microfluidic disk.
A microfluidic microarray that is created in the double spiral format has produced a greater density of probes than in our previous report. Using this double-spiral format together with centrifugal pumping for liquid delivery, 384 x 384 hybridization assays have been performed on one circular disk at one time, at the intersections between the spiral channels and spiral probe lines. Each sample was introduced into each inlet reservoir leading to 4 spiral channels and was analyzed independently, and so the hybridization results were self-corrected among the 4 spiral channels. In this work, fast microarray hybridizations have been successfully achieved by using both complementary oligonucleotides as well as PCR products prepared from plant fungal pathogen cultures.
Chen
Hong
H
Department of Chemistry, Simon Fraser University, Burnaby, British Columbia, CanadaV5A 1S6.
Wang
Lin
L
Li
Paul C H
PC
D001483:Base Sequence; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation* / Q000379:methods*; D009841:Oligonucleotides / Q000032:analysis* / Q000138:chemical synthesis; D016133:Polymerase Chain Reaction / Q000379:methods; D013997:Time Factors
D004129:Dimethylpolysiloxanes; D009841:Oligonucleotides