Comparison between an intradermal skin test and allergen-specific IgE-ELISA for canine atopic dermatitis.
The aim of this study was to compare the results of an intradermal skin test (IDST) with those of an allergen-specific IgE-ELISA in 210 dogs with atopic dermatitis. All the dogs had a clinical diagnosis of atopic dermatitis and underwent an IDST. The sera of all dogs were analysed for allergen-specific IgE by ELISA using the monoclonal antibody D9 against dog IgE. IDST was used as the standard assay. In both methods, the following antigens provided a positive test result: Dermatophagoides farinae, Acarus siro, Tyrophagus putrescentiae, ragweed, mugwort and Lepidoglyphus destructor. ELISA had an overall sensitivity of 82.4% and an overall specificity of 93.8%. The overall accuracy of the ELISA was 91.3%. The evaluated monoclonal D9 ELISA was found to be a reliable tool for the diagnosis of those allergens that cause clinical atopy, and can be recommended for use in dogs when immunotherapy is a therapeutic option.
Tarpataki
N
N
Department and Clinic of Internal Medicine, Faculty of Veterinary Science, Szent István University, Hungary.
Bigler
B
B
Vajdovich
P
P
Vörös
K
K
D000818:Animals; D000911:Antibodies, Monoclonal; D003876:Dermatitis, Atopic / Q000175:diagnosis / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D005260:Female; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007428:Intradermal Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D008297:Male; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D007073:Immunoglobulin E