DNA microarray preparation by light-controlled in situ synthesis.
This unit describes the in situ synthesis of DNA microarrays using a light-controlled process. In a highly parallel fashion, multiple oligonucleotide probes are created from scratch on a glass support using phosphoramidite building blocks carrying a photolabile protecting group. Spatial resolution is achieved by carefully controlling the illumination of defined spots on the glass substrate, on which the oligonucleotide probes are synthesized using straightforward phosphoramidite chemistry.
Beier
Markus
M
Deutsches Krebsforschungszentrum, Heidelberg, Germany.
Hoheisel
Jörg D
JD
D004247:DNA / Q000138:chemical synthesis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry*; D010777:Photochemistry / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
D015345:Oligonucleotide Probes; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D004247:DNA