Selective isolation of mammalian genes by TAR cloning.
Transformation-associated recombination (TAR) cloning provides a unique tool for selective isolation of desired chromosome segments and full-size genes from complex genomes in the form of a circular yeast artificial chromosome (YAC) up to 250 kb in size. The method has a broad application for structural and functional genomics, long-range haplotyping, mutational analysis of gene families, characterization of chromosomal rearrangements, and evolutionary studies. This unit describes a procedure for gene isolation by TAR as well as a method for conversion of YAC-TAR isolates into a bacterial artificial chromosome (BAC) form.
Kouprina
Natalay
N
National Cancer Institute, Bethesda, Maryland, USA.
Larionov
Vladimir
V
D000818:Animals; D003001:Cloning, Molecular / Q000379:methods*; D005796:Genes; D006801:Humans; D008322:Mammals / Q000235:genetics*; D011995:Recombination, Genetic / Q000502:physiology*; D012441:Saccharomyces cerevisiae; D014170:Transformation, Genetic / Q000502:physiology*