Peptides containing T cell epitopes, derived from Sm14, but not from paramyosin, induce a Th1 type of immune response, reduction in liver pathology and partial protection against Schistosoma mansoni infection in mice.
Sm14 and paramyosin are two major Schistosoma mansoni vaccine candidate antigens. Recently, we have identified Sm14 and paramyosin epitopes that are recognized by T cells of resistant individuals living in endemic areas for schistosomiasis. Herein, mice were immunized with these peptides separately or in association in order to evaluate their vaccine potential. Immunization of mice with Sm14 peptides alone or mixed with paramyosin peptides was able to induce 26%-36.7% or 28%-29.2% of worm burden reduction, 67% or 46% of intestinal eggs reduction and also 54%-61% or 43%-52% of liver pathology reduction, respectively. Protection was associated with a Th1 type of immune response induced by Sm14 peptide immunization. In contrast, paramyosin peptide vaccination did not engender protective immunity or liver pathology reduction and immunization was associated with a Th2 type of immune response.
Garcia
Teresa C M
TC
Department of Biochemistry and Immunology and Institute for Investigation in Immunology - Millenium Institute, Federal University of Minas Gerais, Avenue Antonio Carlos 6627, Pampulha, Belo Horizonte, MG, Brazil.
Fonseca
Cristina T
CT
Pacifico
Lucila G G
LG
Durães
Fernanda do Valle
Fdo V
Marinho
Fábio Antônio Vitarelli
FA
Penido
Marcos L O
ML
Caliari
Marcelo V
MV
de Melo
Alan L
AL
Pinto
Hudson Alves
HA
Barsante
Michele Mendes
MM
Cunha-Neto
Edecio
E
Oliveira
Sergio C
SC
D000818:Animals; D000909:Antibodies, Helminth / Q000097:blood; D016207:Cytokines / Q000096:biosynthesis; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D050612:Fatty Acid Transport Proteins / Q000276:immunology*; D005260:Female; D015801:Helminth Proteins / Q000276:immunology*; D007422:Intestines / Q000469:parasitology; D008099:Liver / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010270:Parasite Egg Count; D012550:Schistosoma mansoni / Q000276:immunology; D012555:Schistosomiasis mansoni / Q000517:prevention & control*; D018417:Th1 Cells / Q000276:immunology*; D014335:Tropomyosin / Q000276:immunology*; D022223:Vaccines, Subunit / Q000276:immunology
D000909:Antibodies, Helminth; D016207:Cytokines; D018984:Epitopes, T-Lymphocyte; D050612:Fatty Acid Transport Proteins; D015801:Helminth Proteins; D014335:Tropomyosin; D022223:Vaccines, Subunit; C068709:SM14 protein, Schistosoma mansoni