title: Evaluation of new commercial real-time PCR quantification assay for prenatal diagnosis of cytomegalovirus congenital infection.
abstract: A new commercial real-time human cytomegalovirus (HCMV) PCR kit was evaluated after automated DNA extraction of 153 amniotic fluids in parallel with an in-house real-time PCR assay. The commercial kit displayed 100% sensitivity/specificity compared to the "in-house" assay and was suitable for prenatal diagnosis of HCMV congenital infection.
authors.affiliation: Laboratoire de Virologie, Hôpital Necker-Enfants-malades, 75015 Paris, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Ducroux
authors.forename: Samira
authors.initials: S
authors.lastname: Cherid
authors.forename: Alexandra
authors.initials: A
authors.lastname: Benachi
authors.forename: Yves
authors.initials: Y
authors.lastname: Ville
authors.forename: Marianne
authors.initials: M
authors.lastname: Leruez-Ville
mesh_terms: D000653:Amniotic Fluid / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000175:diagnosis* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D005315:Fetal Diseases / Q000175:diagnosis* / Q000821:virology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis / Q000821:virology; D011296:Prenatal Diagnosis / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D011933:Reagent Kits, Diagnostic