Preparation and maintenance of organotypic cultures for multi-electrode array recordings.
Recording from neuronal cultures with multi-electrode arrays (MEAs) provides a powerful tool for studying neuronal activity with many neurons simultaneously in vitro. This unit describes the detailed steps necessary for growing organotypic cultures on MEAs and the typical neuronal activity that is obtained with this methodology.
Karpiak
Veronica C
VC
Laboratory of Systems Neuroscience, NIMH, NIH, Bethesda, Maryland, USA.
Plenz
Dietmar
D
D000818:Animals; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology; D009924:Organ Culture Techniques / Q000379:methods*; D018408:Patch-Clamp Techniques / Q000379:methods*