title: [Agrobacterium tumefaciens-mediated transformation of the pathogen of Botrytis cinerea].
abstract: Employing gfp as a reporter gene and hygromycin gene (hph) as a selection marker, the recombinant vector pKPG was constructed and transformed into fresh conidia of Botrytis cinerea via Agrobacterium tumefaciens. Transformants were identified by PCR analysis of gfp and hph cassette, green fluorescence observation with microscope and Southern hybridization. Results confirmed that target genes were successfully integrated into the genome of Botrytis cinerea.
authors.affiliation: Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China. weifsh@126.com
authors.forename: Wei-Feng
authors.initials: WF
authors.lastname: Shen
authors.forename: Hong-Biao
authors.initials: HB
authors.lastname: Weng
authors.forename: Bao-Long
authors.initials: BL
authors.lastname: Niu
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: He
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Xiao-Peng
authors.initials: XP
authors.lastname: Qi
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Meng
mesh_terms: D016960:Agrobacterium tumefaciens / Q000235:genetics*; D015139:Blotting, Southern; D020171:Botrytis / Q000235:genetics; D002934:Cinnamates / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic / Q000235:genetics*
chemical_list: D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A