Isotopic labelling of photosystem II in Thermosynechococcus elongatus.
This report describes a protocol to incorporate isotopically labelled aromatic amino acids into the proteins of the thermophilic cyanobacterium Thermosynechoccus elongatus. By using the EPR signal of the two redox active tyrosines of Photosystem II, Tyr(D)(*) and Tyr(Z)(*), as spectroscopic probes it is shown that labelled tyrosines can be incorporated with a high yield in this cyanobacterium. The production of a fully (13)C- or (2)H-labelled enzyme is also described.
Boussac
Alain
A
iBiTec-S, URA CNRS 2096, CEA Saclay, 91191, Gif sur Yvette, France. alain.boussac@cea.fr
Verbavatz
Jean-Marc
JM
Sugiura
Miwa
M
D000458:Cyanobacteria / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D007553:Isotope Labeling; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
D045332:Photosystem II Protein Complex; C066447:tyrosyl radical Y(D); C114979:tyrosyl radical YZ; D014443:Tyrosine