Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface.
Ventral tangential migration of neurons is the most prominent mode of neuronal translocation during earliest neurogenesis in the mouse telencephalon. A typical example of the neurons that adopt this migration mode is guidepost neurons in the lateral olfactory tract designated as lot cells. These neurons are generated from the neocortical neuroepithelium and migrate tangentially down to the ventral edge of the neocortex abutting the ganglionic eminence, on which the future lateral olfactory tract develops. We show here that this migration stream is repelled by a secreted axon guidance molecule, semaphorin 3F through interaction with its specific receptor, neuropilin-2. Accordingly, in mutant mice for semaphorin 3F or neuropilin-2, lot cells ectopically penetrated into the deep brain domain, which normally expresses semaphorin 3F. These results reveal that semaphorin 3F is an important regulator of the ventral tangential migration stream, confining the migrating neurons on the telencephalon surface by repelling from the deeper domain.
Ito
Keisuke
K
Division of Brain Function, National Institute of Genetics, Graduate University for Advanced Studies (Sokendai), Yata 1111, Mishima 411-8540, Japan.
Kawasaki
Takahiko
T
Takashima
Seiji
S
Matsuda
Ikuo
I
Aiba
Atsu
A
Hirata
Tatsumi
T
D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D005260:Female; D006801:Humans; D008565:Membrane Proteins / Q000502:physiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D009833:Olfactory Pathways / Q000166:cytology / Q000502:physiology*; D009924:Organ Culture Techniques; D011247:Pregnancy; D013499:Surface Properties; D013687:Telencephalon / Q000166:cytology / Q000502:physiology*
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C491230:SEMA3F protein, human