Purification and concentration of DNA from aqueous solutions.
This unit presents basic procedures for manipulating solutions of single- or double-stranded DNA through purification and concentration steps. Phenol extraction with ethanol precipitation is described along with an alternate procedure of precipitating DNA with isopropanol. A Support Protocol describes concentration of DNA using butanol.
Moore
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Baylor College of Medicine, Houston, Texas, USA.
D020001:1-Butanol; D019840:2-Propanol; D011232:Chemical Precipitation; D004247:DNA / Q000302:isolation & purification*; D000431:Ethanol; D008967:Molecular Biology / Q000379:methods*; D010636:Phenols; D012996:Solutions
D010636:Phenols; D012996:Solutions; D000431:Ethanol; D020001:1-Butanol; D004247:DNA; D019840:2-Propanol