Detection of phosphorylation by immunological techniques.
Phosphorylation of unlabeled proteins can be detected using immunological or enzymatic techniques. Anti-phosphotyrosine antibodies are used with immunoblots to detect tyrosine phosphorylation. This unit presents a protocol employing anti-phosphotyrosine antibodies with detection by either (125)I-labeled protein A or enhanced chemiluminescence (ECL).
Sefton
B M
BM
The Salk Institute, San Diego, California, USA.
D045163:Antibodies, Phospho-Specific / Q000032:analysis* / Q000276:immunology; D015151:Immunoblotting; D007158:Immunologic Techniques; D007457:Iodine Radioisotopes; D049449:Luminescence; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D013205:Staphylococcal Protein A / Q000378:metabolism
D045163:Antibodies, Phospho-Specific; D007457:Iodine Radioisotopes; D013205:Staphylococcal Protein A; D019000:Phosphotyrosine