Sensitive HPLC-fluorescence detection of morphine labeled with DIB-Cl in rat brain and blood microdialysates and its application to the preliminarily study of the pharmacokinetic interaction between morphine and diclofenac.
A sensitive high-performance liquid chromatography (HPLC)-fluorescence method for determination of morphine (Mor) in rat brain and blood microdialysates was developed using 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride (DIB-Cl) as a label. Mor was labeled with DIB-Cl under mild reaction conditions (at room temperature for 10 min). The separation of DIB-Mor was carried out on an octadecylsilica (ODS) column with CH(3)CN/0.1 M acetate buffer (pH 5.4) within 14 min. The detection limits of Mor in brain and blood microdialysates at a signal-to-noise ratio of 3 were 0.4 and 0.6 ng mL(-1), respectively. The proposed method was successfully applied to the preliminarily study of potential pharmacokinetic interaction between Mor and diclofenac.
Wada
Mitsuhiro
M
Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, Nagasaki, 852-8521, Japan.
Yokota
Chiaki
C
Ogata
Yasuhiro
Y
Kuroda
Naotaka
N
Yamada
Hideyuki
H
Nakashima
Kenichiro
K
D000818:Animals; D001565:Benzoates / Q000737:chemistry*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004008:Diclofenac / Q000493:pharmacokinetics*; D004347:Drug Interactions; D005453:Fluorescence; D007093:Imidazoles / Q000737:chemistry*; D008297:Male; D017551:Microdialysis / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D009020:Morphine / Q000097:blood / Q000737:chemistry / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D012680:Sensitivity and Specificity; D013997:Time Factors
C114684:4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride; D001565:Benzoates; D007093:Imidazoles; D004008:Diclofenac; D009020:Morphine