T cell depletion by cytotoxic elimination.
This unit describes the complete removal of T cells from lymphocyte preparations based on the presence of the glycoprotein Thy-1 on the cell surface of T lymphocytes. As in UNIT 3.3, cytotoxic elimination is employed; however, Thy-1-specific antibodies are used rather than MHC class II-specific antibodies so that T cells are eliminated rather than B cells and accessory cells. Murine T cells can be further fractionated into subpopulations based on their expression of cell differentiation markers CD4 (Leu3, L3T4, T4) and CD8 (Leu2, Lyt2,3). A protocol in this unit describes removal of T cell subsets by cytotoxic elimination using CD-specific antibodies. An alternate but technically more demanding method of enriching T cell subsets is the panning procedure described in UNIT 3.5.
Hathcock
K S
KS
National Cancer Institute, Bethesda, Maryland, USA.
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000918:Antibody Specificity; D015704:CD4 Antigens / Q000276:immunology; D016827:CD8 Antigens / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D003602:Cytotoxicity, Immunologic; D051379:Mice; D013601:T-Lymphocytes / Q000145:classification / Q000276:immunology; D018800:Thy-1 Antigens / Q000276:immunology
D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D016827:CD8 Antigens; D018800:Thy-1 Antigens