Analysis and purification of synthetic nucleic acids using HPLC.
HPLC is a powerful and popular method for analyzing and purifying biomolecules. Reversed-phase HPLC allows a high-capacity method for purification, and uses volatile buffer systems that simplify product recovery. Anion-exchange HPLC provides better resolution and a more predictable elution pattern. This unit presents protocols that are optimized for HPLC of oligonucleotides. Because of the resolution limits of both reversed-phase and anion-exchange HPLC, it can be used for oligonucleotides of up to approximately 50 nt in length.
Andrus
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A
PE Applied Biosystems, Foster City, California, USA.
Kuimelis
R G
RG
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange; D009696:Nucleic Acids / Q000032:analysis* / Q000138:chemical synthesis* / Q000302:isolation & purification; D014320:Triphenylmethyl Compounds
D009696:Nucleic Acids; D014320:Triphenylmethyl Compounds