Left ventricular function in the post-infarct failing mouse heart by magnetic resonance imaging and conductance catheter: a comparative analysis.
OBJECTIVE
Murine myocardial infarction (MI) models are increasingly used in heart failure studies. Magnetic resonance imaging (MRI) and pressure-volume loops by conductance catheter (CC) enable physiological phenotyping. We performed a comparative analysis of MRI vs. CC to assess left ventricular (LV) function in the failing mouse heart.


METHODS
MI was created by LAD ligation. MRI (day 14) and CC (day 15) were used to determine LV end-diastolic volume (EDV), end-systolic volume (ESV) and ejection fraction (EF).


RESULTS
Pooled data yielded moderate-to-strong linear correlations: EDV: R = 0.61; ESV: R = 0.72; EF: R = 0.81. We analysed three groups, no MI (sham, n = 10), small MI (<30% of LV, n = 14) and large MI (>30%, n = 20). Volumes and EF were consistently lower by CC than by MRI, but group differences were evident for both techniques. Receiver-operating characteristic analysis indicated good sensitivity and specificity for both techniques, with superior results for MRI.


CONCLUSIONS
CC and MRI are highly valuable for evaluation of LV volume and function. MRI is recommended for longitudinal studies, accurate absolute volumes and anatomical information. Unique features of CC are its online signal with high temporal resolution, and advanced analysis of LV function and energetics.
Winter
E M
EM
Department of Anatomy and Embryology, Leiden University Medical Center, Leiden, the Netherlands.
Grauss
R W
RW
Atsma
D E
DE
Hogers
B
B
Poelmann
R E
RE
van der Geest
R J
RJ
Tschöpe
C
C
Schalij
M J
MJ
Gittenberger-de Groot
A C
AC
Steendijk
P
P
D000818:Animals; D006328:Cardiac Catheterization / Q000379:methods; D004195:Disease Models, Animal; D004553:Electric Conductivity; D022062:Electrophysiologic Techniques, Cardiac / Q000379:methods; D006333:Heart Failure / Q000209:etiology / Q000503:physiopathology*; D006439:Hemodynamics; D007238:Infarction / Q000150:complications / Q000473:pathology / Q000503:physiopathology*; D008279:Magnetic Resonance Imaging / Q000379:methods; D008297:Male; D051379:Mice; D016513:Mice, SCID; D015203:Reproducibility of Results; D016277:Ventricular Function, Left