Detection of protein-protein interactions by coprecipitation.
Co-precipitation of proteins from whole cell extracts is a valuable approach to test for physical interactions between proteins of interest. It may be the method of choice, or may be used in conjunction with other procedures to detect protein-protein interactions, such as two-hybrid analyis and co-purification schemes, and tests of physical associations using purified proteins.
Elion
Elaine A
EA
Harvard Medical School, Boston, Massachusetts.
D011232:Chemical Precipitation; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000378:metabolism*
D011993:Recombinant Fusion Proteins