title: Protein identification using a quadrupole ion trap mass spectrometer and SEQUEST database matching.
abstract: A procedure is described for rapid, sensitive protein identification utilizing liquid chromatography--tandem mass spectrometry. The analysis is performed on mixtures of peptides obtained by enzyme digestion. The SEQUEST computer program is used to match the sequence information in the spectra to a database of known protein sequences.
authors.affiliation: Beckman Research Institute of the City of Hope, Duarte, California, USA.
authors.forename: R E
authors.initials: RE
authors.lastname: Moore
authors.forename: M K
authors.initials: MK
authors.lastname: Young
authors.forename: T D
authors.initials: TD
authors.lastname: Lee
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D011506:Proteins / Q000032:analysis / Q000737:chemistry*; D015203:Reproducibility of Results; D012984:Software; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011506:Proteins