title: Proteomic identification of p53-dependent protein phosphorylation.
abstract: The p53 tumor suppressor regulates transcription of target genes. We have previously analysed the p53-dependent proteome and identified novel protein targets. Here we have examined p53-dependent phosphorylation using two-dimensional gel electrophoresis and staining with the fluorescent phosphoprotein dye Pro-Q Diamond. We report that p53 induces phosphorylation of a subset of proteins including Nm23, DJ-1, ANXA1 and PrxII. Our identification of p53-dependent phosphorylation of specific target proteins reveals new aspects of the p53-dependent cellular response and suggests that such posttranslational modifications may contribute to p53-mediated tumor suppression.
authors.affiliation: Department of Oncology-Pathology, Cancer Center Karolinska, Karolinska Institutet, Stockholm, Sweden.
authors.forename: R
authors.initials: R
authors.lastname: Rahman-Roblick
authors.forename: U
authors.initials: U
authors.lastname: Hellman
authors.forename: S
authors.initials: S
authors.lastname: Becker
authors.forename: F G
authors.initials: FG
authors.lastname: Bader
authors.forename: G
authors.initials: G
authors.lastname: Auer
authors.forename: K G
authors.initials: KG
authors.lastname: Wiman
authors.forename: U J
authors.initials: UJ
authors.lastname: Roblick
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D016685:Mitomycin / Q000494:pharmacology; D010766:Phosphorylation; D040901:Proteomics; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D016159:Tumor Suppressor Protein p53; D016685:Mitomycin