title: Immunophenotype characterization of rat mesenchymal stromal cells.
abstract: BACKGROUND
  Mesenchymal stromal cells (MSC) have shown diverse therapeutic potential. While characterization of human and mouse MSC has seen significant advances, rat bone marrow-derived MSC (rBM-MSC) remain under-characterized. We detail the isolation, expansion, differentiation, and detailed immunocharacterization of rBM-MSC.
  
  
  METHODS
  Rat MSC were isolated and expanded in multipotent adult progenitor cell (MAPC) media, and cell-surface marker expression through 10 passages was used to characterize the population and multipotency was confirmed via differentiation.
  
  
  RESULTS
  By passage 3, rBM-MSC were found to be CD11b-, CD45-, CD29+, CD49e+, CD73+, CD90+, CD105+ and Stro-1+, without the use of cell sorting. Media selection was responsible for the isolation of a nearly homogeneous population of rBM-MSC. The rBM-MSC immunophenotype changed by passage 10, showing decreases in CD73, CD105 and Stro-1 expression.
  
  
  CONCLUSIONS
  Detailed characterization of cell populations facilitates accurate and reproducible cell therapy investigation. Given the expanding body of research involving rBM-MSC, these results advance our ability to compare rBM-MSC populations.
authors.affiliation: Department of Pediatric Surgery, University of Texas Medical School at Houston, Houston, TX 77030, USA.
authors.forename: Mt
authors.initials: M
authors.lastname: Harting
authors.forename: F
authors.initials: F
authors.lastname: Jimenez
authors.forename: S
authors.initials: S
authors.lastname: Pati
authors.forename: J
authors.initials: J
authors.lastname: Baumgartner
authors.forename: Cs
authors.initials: C
authors.lastname: Cox
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology; D002454:Cell Differentiation; D049109:Cell Proliferation; D019902:Chondrocytes / Q000166:cytology; D003470:Culture Media; D016130:Immunophenotyping; D008648:Mesoderm / Q000166:cytology*; D010011:Osteocytes / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017154:Stromal Cells / Q000166:cytology
chemical_list: D015415:Biomarkers; D003470:Culture Media