title: Solid-phase oligonucleotide labeling with DOTA.
abstract: This unit describes a method to construct oligonucleotide conjugates labeled with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) on a solid support. A nucleosidic phosphoramidite that contains a protected DOTA ligand compatible with normal chain assembly is prepared first. As the chain assembly is completed, the oligonucleotide is deprotected and converted to the corresponding gadolinium(III) chelate, resulting in an oligonucleotide conjugate containing the chelate at the 5'-terminus.
authors.affiliation: PerkinElmer Life and Analytical Sciences, Turku, Finland.
authors.forename: Lassi
authors.initials: L
authors.lastname: Jaakkola
authors.forename: Alice
authors.initials: A
authors.lastname: Ylikoski
authors.forename: Jari
authors.initials: J
authors.lastname: Hovinen
mesh_terms: D000577:Amides / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D009841:Oligonucleotides / Q000737:chemistry*; D010756:Phosphoric Acids / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000577:Amides; D006573:Heterocyclic Compounds, 1-Ring; D009841:Oligonucleotides; D010756:Phosphoric Acids; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid; C011067:phosphoramidic acid