Hzf regulates adipogenesis through translational control of C/EBPalpha.
Adipocyte differentiation requires a well-defined programme of gene expression in which the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein) has a central function. Here, we show that Hzf (haematopoietic zinc-finger), a previously identified p53 transcriptional target, regulates C/EBPalpha expression. Hzf is induced during differentiation of preadipocyte cell lines, and its suppression by short hairpin RNA disrupts adipogenesis. In Hzf's absence, expression of C/EBPalpha is severely impaired because of reduced translation of its mRNA. Hzf physically interacts with the 3' untranslated region of C/EBPalpha mRNA to enhance its translation. Taken together, these findings underscore a critical role of Hzf in the adipogenesis regulatory cascade.
Kawagishi
Hiroyuki
H
Department of Mechanism of Aging, National Institute for Longevity Sciences, Aichi, Japan.
Wakoh
Takeshi
T
Uno
Hatsume
H
Maruyama
Mitsuo
M
Moriya
Ayako
A
Morikawa
Satoru
S
Okano
Hideyuki
H
Sherr
Charles J
CJ
Takagi
Masatoshi
M
Sugimoto
Masataka
M
D020413:3' Untranslated Regions / Q000378:metabolism; D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000235:genetics*; D000818:Animals; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism; D006801:Humans; D007333:Insulin Resistance; D051379:Mice; D018345:Mice, Knockout; D014176:Protein Biosynthesis / Q000235:genetics; D011506:Proteins / Q000235:genetics / Q000378:metabolism*
D020413:3' Untranslated Regions; D022763:CCAAT-Enhancer-Binding Protein-alpha; D011506:Proteins; C404217:Zfp385 protein, mouse; D005947:Glucose