title: Efficient synthesis of functionalized oligodeoxyribonucleotides with base-labile groups using a new silyl linker.
abstract: In this study, we developed new 3'-terminal deoxyribonucleoside-loading reagents 1 with a new silyl-type linker. These reagents could increase the efficiency of introduction of 3'-terminal deoxyribonucleoside components into polymer supports to a level of 17-29micromol/g. The efficiency was higher than that of previous T-loading reagents because reagents 1 contain a 4-aminobutyryl residue as a spacer. Moreover, we could synthesize not only unmodified DNA oligomers but also a base-labile modified DNA oligomer using resins 9a-d in the activated phosphite method without base protection.
authors.affiliation: Department of Life Science, Tokyo Institute of Technology and CREST, JST (Japan Science and Technology Agency), Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ohkubo
authors.forename: Rintaro
authors.initials: R
authors.lastname: Kasuya
authors.forename: Katsufumi
authors.initials: K
authors.lastname: Aoki
authors.forename: Akio
authors.initials: A
authors.lastname: Kobori
authors.forename: Haruhiko
authors.initials: H
authors.lastname: Taguchi
authors.forename: Kohji
authors.initials: K
authors.lastname: Seio
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Sekine
mesh_terms: D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D012821:Silanes / Q000138:chemical synthesis / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D009838:Oligodeoxyribonucleotides; D012821:Silanes