Protective effect of trihexyphenidyl on hydrogen peroxide-induced oxidative damage in PC12 cells.
The protective effect of trihexyphenidyl (THY) on hydrogen peroxide-induced oxidative damage was investigated in the rat pheochromocytoma line PC12 cells. Following the exposure of PC12 cells to H(2)O(2), there was a reduction in cell survival, activities of superoxide dismutase (SOD) and mitochondria membrane potential (MMP), in contrast, the increased levels in Lactate dehydrogenase (LDH) release, malondialdehyde (MDA) production and intracellular reactive oxygen species (ROS), as well as intracellular [Ca(2+)]i level were observed. However, preincubation of cells with THY prior to H(2)O(2) exposure attenuated all the changes mentioned above, THY exhibited protective effect against H(2)O(2)-induced toxicity in PC12 cells, indicating that the compound may be a potential therapeutic agent for the diseases influenced by oxidative damage.
Ji
Bian-Sheng
BS
Institute of Pharmacy, Henan University, Kaifeng 475001, China. jibiansheng@yahoo.com.cn
Gao
Yuan
Y
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D000978:Antiparkinson Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D005434:Flow Cytometry; D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide / Q000633:toxicity*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects; D051336:Mitochondrial Membranes / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D013778:Tetrazolium Salts; D013844:Thiazoles; D014282:Trihexyphenidyl / Q000494:pharmacology*; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D000978:Antiparkinson Agents; D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; D014810:Vitamin E; D008315:Malondialdehyde; D014282:Trihexyphenidyl; D006861:Hydrogen Peroxide; D007770:L-Lactate Dehydrogenase; D013482:Superoxide Dismutase; C022616:thiazolyl blue