title: Recent advances in on-line coupling of capillary electrophoresis to atomic absorption and fluorescence spectrometry for speciation analysis and studies of metal-biomolecule interactions.
abstract: Speciation information is vital for the understanding of the toxicity, mobility and bioavailability of elements in environmental or biological samples. Hyphenating high resolving power of separation techniques and element-selective detectors provides powerful tools for studying speciation of trace elements in environmental and biological systems. During the last five years several novel hybrid techniques based on capillary electrophoresis (CE) and atomic spectrometry have been developed for speciation analysis and metal-biomolecule interaction study in our laboratory. These techniques include CE on-line coupled with atomic fluorescence spectrometry (AFS), chip-CE on-line coupled with AFS, CE on-line coupled with flame heated quartz furnace atomic absorption spectrometry (FHF-AAS), and CE on-line coupled with electrothermal atomic absorption spectrometry (ETAAS). The necessity for the development of these techniques, their interface design, and applications in speciation analysis and metal-biomolecule interaction study are reviewed. The advantages and limitations of the developed hybrid techniques are critically discussed, and further development is also prospected.
authors.affiliation: Research Center for Analytical Sciences, College of Chemistry, Nankai University, 94 Weijin Road, Tianjin 300071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.forename: Xue-Bo
authors.initials: XB
authors.lastname: Yin
authors.forename: Xiu-Ping
authors.initials: XP
authors.lastname: Yan
mesh_terms: D019075:Electrophoresis, Capillary / Q000295:instrumentation; D004784:Environmental Monitoring; D053768:Metal Nanoparticles / Q000737:chemistry*; D008767:Methylmercury Compounds / Q000032:analysis / Q000737:chemistry; D046210:Microfluidic Analytical Techniques; D013050:Spectrometry, Fluorescence / Q000295:instrumentation; D013054:Spectrophotometry, Atomic / Q000295:instrumentation; D014835:Volatilization
chemical_list: D008767:Methylmercury Compounds