Effects of scutellarin on apoptosis induced by cobalt chloride in PC12 cells.
The present study investigated the protective effects of scutellarin on cobalt chloride (CoCl2)-induced apoptosis in PC12 cells. Incubation of PC12 cells with 500 microM CoCl2 for 24 h resulted in significant apoptosis as evaluated by the crystal violet, electron microscopy and flow cytometry assays. The increase of caspase-3 activity, decrease of Bcl-XL expression, phosphorylation of p38 mitogen-activated protein kinase (MAPK) and accumulation of intracellular reactive oxygen species (ROS) were also seen in CoCl2-treated PC12 cells. Scutellarin at 0.1, 1 and 10 microM significantly protected against the apoptotic cell death induced by CoCl2. Scutellarin decreased caspase-3 activity, increased Bcl-XL expression, inhibited p38 phosphorylation and attenuated ROS production. These results demonstrate that scutellarin can protect PC12 cells from cobalt chloride induced apoptosis by scavenging ROS, inhibiting p38 phosphorylation, up-regulating Bcl-XL expression and decreasing caspase-3 activity, and may reduce the cellular damage in pathological conditions associated with hypoxia-mediated neuronal injury.
Wang
Li-Xiang
LX
Institute of Pharmacology, School of Medicine, Shandong University Jinan 250012, Shandong, PR China.
Zeng
Ji-Ping
JP
Wei
Xin-Bing
XB
Wang
Fu-Wu
FW
Liu
Zhao-Ping
ZP
Zhang
Xiu-Mei
XM
D000818:Animals; D047310:Apigenin / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D061945:Caspase Inhibitors; D002470:Cell Survival; D003035:Cobalt / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D005434:Flow Cytometry; D005965:Glucuronates / Q000494:pharmacology*; D007202:Indicators and Reagents; D008854:Microscopy, Electron; D016716:PC12 Cells; D010766:Phosphorylation; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics; D051020:bcl-X Protein / Q000096:biosynthesis; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D061945:Caspase Inhibitors; D005965:Glucuronates; D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D051020:bcl-X Protein; C484876:scutellarin; D003035:Cobalt; D047310:Apigenin; D048051:p38 Mitogen-Activated Protein Kinases; C018021:cobaltous chloride