title: Diminished frequency and function of CD4+CD25high regulatory T cells associated with active uveitis in Vogt-Koyanagi-Harada syndrome.
abstract: OBJECTIVE
  CD4(+)CD25(high) regulatory T (Treg) cells have been shown to be involved in the pathogenesis of autoimmune diseases. Vogt-Koyanagi-Harada (VKH) syndrome is an organ-specific autoimmune disease. This study was designed to phenotypically and functionally characterize peripheral blood CD4(+)CD25(high) Treg cells in VKH patients with active uveitis.
  
  
  METHODS
  Blood samples were taken from 30 patients with active VKH, 19 patients with inactive VKH, and 26 healthy controls. Peripheral blood mononuclear cells were subjected to flow cytometry for analysis of phenotypes of the CD4(+)CD25(high) Treg cells. For functional analysis, CD4(+)CD25(high) Treg cells and CD4(+)CD25(-) T cells were separated by means of magnetic-assisted cell sorting and subsequently cocultured for 6 days. The proliferation of CD4(+)CD25(-) T cells was measured by [(3)H] thymidine incorporation assay. The levels of IFN-gamma, IL-17, and IL-13 in the supernatants were determined by enzyme-linked immunosorbent assay.
  
  
  RESULTS
  Significantly decreased frequencies of CD4(+)CD25(high) Treg cells and percentages of FOXP3(+) cells in these Treg cells were shown in patients with active VKH. Treg cells from patients with active VKH showed a significant deficiency in suppressing the proliferation of CD4(+)CD25(-) T cells and inhibiting the production of IFN-gamma and IL-13 by CD4(+)CD25(-) T cells. CD4(+)CD25(high) Treg cells from VKH patients or healthy controls did not markedly inhibit or promote IL-17 production.
  
  
  CONCLUSIONS
  A significantly decreased frequency and diminished function of CD4(+)CD25(high) Treg cells is associated with active uveitis in patients with VKH syndrome. These results suggest that these dysfunctional CD4(+)CD25(high) Treg cells may play a role in the pathogenesis of uveitis in VKH syndrome.
authors.affiliation: State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Uveitis Study Center of Sun Yat-sen University, Guangzhou, People's Republic of China.
authors.forename: Lina
authors.initials: L
authors.lastname: Chen
authors.forename: Peizeng
authors.initials: P
authors.lastname: Yang
authors.forename: Hongyan
authors.initials: H
authors.lastname: Zhou
authors.forename: Hao
authors.initials: H
authors.lastname: He
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Ren
authors.forename: Wei
authors.initials: W
authors.lastname: Chi
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Aize
authors.initials: A
authors.lastname: Kijlstra
mesh_terms: D000328:Adult; D001327:Autoimmune Diseases / Q000276:immunology*; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D018189:Immunomagnetic Separation; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism; D020381:Interleukin-17 / Q000378:metabolism; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology*; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*; D014605:Uveitis / Q000276:immunology*; D014607:Uveomeningoencephalitic Syndrome / Q000276:immunology*
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; D018793:Interleukin-13; D020381:Interleukin-17; D053645:Interleukin-2 Receptor alpha Subunit; D007371:Interferon-gamma