Biochemical mechanism of healing activity of the natural phenolic, allylpyrocatechol against indomethacin-induced gastric ulceration in mice.
Indomethacin caused maximum stomach ulceration in mice on the 3rd day, which was associated with reduction of plasma total antioxidant status (TAS), COX-1, COX-2, mucosal PGE(2), VEGF, and vWF, along with an increase in endostatin levels. Treatment with the phytochemical allylpyrocatechol (5 mg/kg, p.o. for 3 days) provided significant ulcer healing by reversing these biochemical parameters, as well as increasing the EGF expression more than that observed due to ulceration. Omeprazole (3 mg/kg, p.o. for 3 days) provided a similar healing by improving TAS and mucin levels, without significantly altering the other parameters.
Banerjee
Debashish
D
Department of Biochemistry, Dr. B.C. Roy Post Graduate Institute of Basic Medical Sciences, Kolkata, 700 020, India.
Bhattacharya
Sayanti
S
Bandyopadhyay
Sandip K
SK
Chattopadhyay
Subrata
S
D000818:Animals; D000897:Anti-Ulcer Agents / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology / Q000627:therapeutic use*; D051545:Cyclooxygenase 1 / Q000378:metabolism; D051546:Cyclooxygenase 2 / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007213:Indomethacin; D008297:Male; D051379:Mice; D009853:Omeprazole / Q000494:pharmacology / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects / Q000502:physiology*; D014841:von Willebrand Factor / Q000378:metabolism
D000897:Anti-Ulcer Agents; D000975:Antioxidants; D002396:Catechols; D010936:Plant Extracts; D042461:Vascular Endothelial Growth Factor A; C523074:allylpyrocatechol; D014841:von Willebrand Factor; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D009853:Omeprazole; D007213:Indomethacin