title: Isolation and characterization of an Isaria fumosorosea isolate infecting the Asian citrus psyllid in Florida.
abstract: A fungal pathogen that killed adult Diaphorina citri Kuwayama (Asian citrus psyllid) (Hemiptera: Psyllidae) in Florida citrus groves during the fall of 2005 was identified and characterized. Investigation of this pathogen is important because D. citri vectors citrus greening disease (Huanglongbing), which was reported in Florida in 2005. The morphological and genetic data generated herein support identification of the fungus as Isaria fumosorosea Wize (Ifr) (=Paecilomyces fumosoroseus) (Hypocreales: Cordycipitaceae) from the Asian citrus psyllid (Ifr AsCP). Koch's postulates were fulfilled after the fungus was isolated in vitro and transmitted to healthy psyllids, which then exhibited a diseased-phenotype similar to that observed in the field. Both in vitro growth characteristics and two Ifr AsCP-specific molecular markers discriminated the psyllid pathogen from another local Ifr isolate, Ifr 97 Apopka. These molecular markers will be useful to track the dynamics of this disease in D. citri populations. The potential for utilizing Ifr to complement existing psyllid pest management strategies is discussed.
authors.affiliation: Department of Entomology and Nematology, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611-0620, USA. meyerjm@purdue.edu
authors.forename: Jason M
authors.initials: JM
authors.lastname: Meyer
authors.forename: Marjorie A
authors.initials: MA
authors.lastname: Hoy
authors.forename: Drion G
authors.initials: DG
authors.lastname: Boucias
mesh_terms: D000818:Animals; D004271:DNA, Fungal / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D006430:Hemiptera / Q000382:microbiology* / Q000502:physiology; D007300:Insect Control / Q000379:methods; D007303:Insect Vectors; D008969:Molecular Sequence Data; D009181:Mycoses / Q000503:physiopathology* / Q000635:transmission; D010058:Oviposition; D010143:Paecilomyces / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D017422:Sequence Analysis, DNA
chemical_list: D004271:DNA, Fungal; D005819:Genetic Markers