Protein detection in gels using fixation.
This unit has been recently updated to include information on preformulated gel stains as well as new protocols for Sypro Ruby and silver staining, and gel imaging methodology. Other previously published protocols are also provided for both rapid and acid-based Coomassie blue staining, and alternate methods for silver staining (i.e., nonammoniacal silver staining, rapid silver staining, and an enhanced-background, two-stage method).
Echan
Lynn A
LA
The Wistar Institute, Philadelphia, Pennsylvania.
Speicher
David W
DW
The Wistar Institute, Philadelphia, Pennsylvania.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D007091:Image Processing, Computer-Assisted; D009942:Organometallic Compounds / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D016622:Silver Staining / Q000379:methods; D013194:Staining and Labeling / Q000379:methods*
D009942:Organometallic Compounds; D011506:Proteins; C515601:Sypro Ruby