Regulation of glia number in Drosophila by Rap/Fzr, an activator of the anaphase-promoting complex, and Loco, an RGS protein.
Glia mediate a vast array of cellular processes and are critical for nervous system development and function. Despite their immense importance in neurobiology, glia remain understudied and the molecular mechanisms that direct their differentiation are poorly understood. Rap/Fzr is the Drosophila homolog of the mammalian Cdh1, a regulatory subunit of the anaphase-promoting complex/cyclosome (APC/C). APC/C is an E3 ubiquitin ligase complex well characterized for its role in cell cycle progression. In this study, we have uncovered a novel cellular role for Rap/Fzr. Loss of rap/fzr function leads to a marked increase in the number of glia in the nervous system of third instar larvae. Conversely, ectopic expression of UAS-rap/fzr, driven by repo-GAL4, results in the drastic reduction of glia. Data from clonal analyses using the MARCM technique show that Rap/Fzr regulates the differentiation of surface glia in the developing larval nervous system. Our genetic and biochemical data further indicate that Rap/Fzr regulates glial differentiation through its interaction with Loco, a regulator of G-protein signaling (RGS) protein and a known effector of glia specification. We propose that Rap/Fzr targets Loco for ubiquitination, thereby regulating glial differentiation in the developing nervous system.
Kaplow
Margarita E
ME
Department of Biology, City College and The Graduate Center, City University of New York, New York 10031, USA.
Korayem
Adam H
AH
Venkatesh
Tadmiri R
TR
D064173:Anaphase-Promoting Complex-Cyclosome; D000818:Animals; D017209:Apoptosis; D001921:Brain / Q000254:growth & development / Q000378:metabolism; D064200:Cdh1 Proteins; D002452:Cell Count; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D019070:Cell Lineage; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000166:cytology*; D005123:Eye / Q000254:growth & development / Q000378:metabolism; D007814:Larva / Q000254:growth & development / Q000378:metabolism; D008954:Models, Biological; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D011485:Protein Binding; D021381:Protein Transport; D020710:RGS Proteins / Q000378:metabolism*; D043743:Ubiquitin-Protein Ligase Complexes / Q000378:metabolism*
D064200:Cdh1 Proteins; D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C119015:LOCO protein, Drosophila; D009419:Nerve Tissue Proteins; D020710:RGS Proteins; C577674:fzr protein, Drosophila; D043743:Ubiquitin-Protein Ligase Complexes; D064173:Anaphase-Promoting Complex-Cyclosome