title: Gene transduction by sonoporation.
abstract: Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relatively simple procedures and easily transduces genes into mesenchymal cells without significant damage to target tissues. Therefore, sonoporation is effective for gene transduction to study the molecular mechanisms of morphogenesis.
authors.affiliation: Department of Neurobiology and Anatomy, School of Medicine, University of Utah, Salt Lake City, Utah, USA.
authors.forename: Sho
authors.initials: S
authors.lastname: Ohta
authors.forename: Kentaro
authors.initials: K
authors.lastname: Suzuki
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Ogino
authors.forename: Shinichi
authors.initials: S
authors.lastname: Miyagawa
authors.forename: Aki
authors.initials: A
authors.lastname: Murashima
authors.forename: Daisuke
authors.initials: D
authors.lastname: Matsumaru
authors.forename: Gen
authors.initials: G
authors.lastname: Yamada
mesh_terms: D000818:Animals; D002642:Chick Embryo; D018274:Electroporation / Q000379:methods; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018878:Limb Buds / Q000196:embryology; D008648:Mesoderm / Q000166:cytology; D045423:Microbubbles; D008957:Models, Genetic; D013010:Sonication; D014161:Transduction, Genetic
chemical_list: D049452:Green Fluorescent Proteins