title: Automated fluorescent genotyping.
abstract: With the publication of a comprehensive human genetic map consisting of over 5000 microsatellite markers, reagents are in hand to undertake large-scale genotyping projects. This unit describes the basic methodology, optimization of markers, and allele calling. With the publication of a comprehensive human genetic map consisting of over 5000 microsatellite markers, reagents are in hand.
authors.affiliation: Sequana Therapeutics, Inc., La Jolla, California, USA.
authors.forename: J
authors.initials: J
authors.lastname: Hall
authors.forename: E
authors.initials: E
authors.lastname: Nanthakumar
mesh_terms: D001331:Automation; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D005819:Genetic Markers; D005821:Genetic Techniques; D005826:Genetics, Medical; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D005819:Genetic Markers; D004247:DNA