[Ultrasonic extraction for flavonoids from Perilla frutescens by response surface method].
OBJECTIVE
To investigate the extraction process of Perilla frutescens flavonoids.


METHODS
Response surface method (RSM) was applied to a fractional design. The Perillafrutescens flavonoids were extracted by ultrasonic method. The influence factors of concentration of alcohol, ratio of liquid to solid, extraction times were evaluated.


RESULTS
The optimum extraction conditions were confirmed as follows: 60% alcohol extracted 4 times, 18 minutes every time and ratio of liquid to solid was 15:1. The extraction rate of flavonoids under this condition was 24. 65 mg/g.


CONCLUSIONS
Applying ultrasonic method to extract Perilla frutescens flavonoids is feasible.
Wang
Wei
W
Department of Pharmacy, Zhejiang University of Traditional Chinese Medicine, Hangzhou 310053, China.
Yu
Chen-huan
CH
Shen
Jie
J
Wu
Qiao-feng
QF
D000704:Analysis of Variance; D005419:Flavonoids / Q000032:analysis / Q000302:isolation & purification*; D006801:Humans; D019686:Lamiaceae / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011208:Powders; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors; D014465:Ultrasonics
D005419:Flavonoids; D011208:Powders