title: Single cell electroporation method for axon tracing in cultured slices.
abstract: Axon tracing is an essential method to reveal neuronal connection patterns and characteristics of growing axons during development. Here we introduce an electroporation-based gene transfer technique with a fluorescent protein (FP), which enables us to observe a small number of axons. The result also demonstrates that dynamics of axon behavior can be followed for more than a week. Thus, such labeling with FP is a powerful tool for axon tracing.
authors.affiliation: Neuroscience Laboratories, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka, Japan.
authors.forename: Naofumi
authors.initials: N
authors.lastname: Uesaka
authors.forename: Maki
authors.initials: M
authors.lastname: Nishiwaki
authors.forename: Nobuhiko
authors.initials: N
authors.lastname: Yamamoto
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism / Q000502:physiology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000196:embryology*; D003712:Dendrites / Q000378:metabolism; D015509:Developmental Biology / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008164:Luminescent Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008164:Luminescent Proteins; D049452:Green Fluorescent Proteins