title: Exogenous stromal cell-derived factor-1 induces modest leukocyte recruitment in vivo.
abstract: Stromal cell-derived factor-1 (SDF-1; CXCL12), a CXC chemokine, has been found to be involved in inflammation models in vivo and in cell adhesion, migration, and chemotaxis in vitro. This study aimed to determine whether exogenous SDF-1 induces leukocyte recruitment in mice. After systemic administration of SDF-1alpha, expression of the adhesion molecules P-selectin and VCAM-1 in mice was measured using a quantitative dual-radiolabeled Ab assay and leukocyte recruitment in various tissues was evaluated using intravital microscopy. The effect of local SDF-1alpha on leukocyte recruitment was also determined in cremaster muscle and compared with the effect of the cytokine TNFalpha and the CXC chemokine keratinocyte-derived chemokine (KC; CXCL1). Systemic administration of SDF-1alpha (10 microg, 4-5 h) induced upregulation of P-selectin, but not VCAM-1, in most tissues in mice. It caused modest leukocyte recruitment responses in microvasculature of cremaster muscle, intestine, and brain, i.e., an increase in flux of rolling leukocytes in cremaster muscle and intestines, leukocyte adhesion in all three tissues, and emigration in cremaster muscle. Local treatment with SDF-1alpha (1 microg, 4-5 h) reduced leukocyte rolling velocity and increased leukocyte adhesion and emigration in cremasteric venules, but the responses were much less profound than those elicited by KC or TNFalpha. SDF-1alpha-induced recruitment was dependent on endothelial P-selectin, but not P-selectin on platelets. We conclude that the exogenous SDF-1alpha enhances leukocyte-endothelial cell interactions and induces modest and endothelial P-selectin-dependent leukocyte recruitment.
authors.affiliation: Department of Pharmacology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Kerfoot
authors.forename: Graciela
authors.initials: G
authors.lastname: Andonegui
authors.forename: Claudine S
authors.initials: CS
authors.lastname: Bonder
authors.forename: Lixin
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D001792:Blood Platelets / Q000276:immunology; D001921:Brain / Q000098:blood supply*; D002448:Cell Adhesion; D054360:Chemokine CXCL1 / Q000378:metabolism; D054377:Chemokine CXCL12 / Q000008:administration & dosage / Q000378:metabolism*; D004151:Dipeptides / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects / Q000276:immunology*; D007249:Inflammation / Q000276:immunology; D007274:Injections, Intraperitoneal; D053583:Interleukin-1beta / Q000378:metabolism; D007421:Intestine, Small / Q000098:blood supply*; D036904:Leukocyte Rolling / Q000187:drug effects; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008833:Microcirculation / Q000276:immunology; D018715:Microscopy, Video; D018482:Muscle, Skeletal / Q000098:blood supply*; D019007:P-Selectin / Q000378:metabolism; D011480:Protease Inhibitors / Q000494:pharmacology; D011994:Recombinant Proteins / Q000378:metabolism*; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
chemical_list: D054360:Chemokine CXCL1; D054377:Chemokine CXCL12; C515256:Cxcl12 protein, mouse; D004151:Dipeptides; D053583:Interleukin-1beta; C078131:N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide; D019007:P-Selectin; D011480:Protease Inhibitors; D011994:Recombinant Proteins; D014409:Tumor Necrosis Factor-alpha; D019010:Vascular Cell Adhesion Molecule-1