Protection of 5'-hydroxy functions of nucleosides.
The 5-hydroxy group is the primary hydroxy group of nucleosides. It is mandatory to protect 5-hydroxyls in all methods of oligonucleotide synthesis that require nucleoside synthons. This unit discusses a wide variety of acid-labile and base-labile protecting groups, as well as enzymatic methods for 5-protection and deprotection.
Seliger
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University of Ulm, Ulm, Germany.
D000143:Acids; D000215:Acylation; D001671:Biochemistry / Q000379:methods*; D002384:Catalysis; D004798:Enzymes / Q000378:metabolism; D005456:Fluorescent Dyes; D017665:Hydroxyl Radical / Q000737:chemistry*; D008024:Ligands; D009705:Nucleosides / Q000737:chemistry*; D010766:Phosphorylation; D014320:Triphenylmethyl Compounds / Q000737:chemistry
D000143:Acids; D004798:Enzymes; D005456:Fluorescent Dyes; D008024:Ligands; D009705:Nucleosides; D014320:Triphenylmethyl Compounds; D017665:Hydroxyl Radical