Protective effect of vanilloids against tert-butyl hydroperoxide-induced oxidative stress in vero cells culture.
This study investigated the effect of synthetic capsiate, a simplified analogue of capsiate, and vanillyl alcohol on the oxidative stress induced by tert-butyl hydroperoxide (TBH) in a line of fibroblasts derived from monkey kidney (Vero cells). In response to the TBH-mediated oxidative stress, a reduction of the levels of total unsaturated fatty acids and cholesterol was observed, and a rise in the concentrations of conjugated dienes fatty acids hydroperoxides and 7-ketocholesterol. Pretreatment with both synthetic capsiate and vanillyl alcohol preserved Vero cells from oxidative damage and showed a remarkable protective effect on the reduction of the levels of total unsaturated fatty acids and cholesterol, inhibiting the increase of MDA, conjugated dienes fatty acids hydroperoxides, and 7-ketocholesterol. Both compounds were effective against peroxidation of cell membrane lipids induced by TBH, with synthetic capsiate essentially acting as a pro-drug of vanillyl alcohol, its hydrophilic hydrolytic derivative.
Rosa
Antonella
A
Dip. Biologia Sperimentale, Sez. Patologia Sperimentale, Università degli Studi di Cagliari, Cittadella Universitaria, SS 554, Km 4.5, 09042 Monserrato, Cagliari, Italy. anrosa@unica.it
Atzeri
Angela
A
Deiana
Monica
M
Melis
M Paola
MP
Incani
Alessandra
A
Corona
Giulia
G
Loru
Debora
D
Appendino
Giovanni
G
Dessì
M Assunta
MA
D000818:Animals; D001592:Benzyl Alcohols / Q000032:analysis / Q000494:pharmacology*; D002211:Capsaicin / Q000031:analogs & derivatives* / Q000032:analysis / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002522:Chlorocebus aethiops; D002784:Cholesterol / Q000032:analysis; D005231:Fatty Acids, Unsaturated / Q000032:analysis; D005347:Fibroblasts / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000032:analysis; D018384:Oxidative Stress / Q000187:drug effects*; D014709:Vero Cells; D020122:tert-Butylhydroperoxide / Q000494:pharmacology*
D001592:Benzyl Alcohols; D005231:Fatty Acids, Unsaturated; D008315:Malondialdehyde; D020122:tert-Butylhydroperoxide; D002784:Cholesterol; C470213:capsiate; D002211:Capsaicin; C024078:vanillyl alcohol