title: [Isolation of a thermophilic bacterium and analysis of its extracellular polymeric substance].
abstract: A thermophilic bacterial strain MS-1 was isolated from the water let from an oil well, and identified as Bacillus sp. by its 16S rDNA sequence and morphological characteristics. The bacterium was able to grow at 60 degrees C and produce extracellular polymeric substance (EPS). Analysis of the EPS showed that there was 48.3% - 54.5% polysaccharide, which was composed of mannose:glucose:galactose at a ratio of 2.04 : 1.00 : 0.89. The protein fraction was 37.2% - 42.4% in EPS, containing methionine, leucine, aspartic acid, alanine, histidine and serine.Transmission Electron Microscopy and Environmental Scanning Electron Microscopy were used to observe the extracellular polymeric substances. The strain is useful in microbial profile modification in high temperature oil fields.
authors.affiliation: College of Life Sciences, Nankai University, Tianjin Key Laboratory of Microbial Functional Genomics, Ministry of Education, Tianjin 300071, China. liujing8660@mail.nankai.edu.cn
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.forename: Xinyu
authors.initials: X
authors.lastname: Bai
authors.forename: Ting
authors.initials: T
authors.lastname: Ma
authors.forename: Fenglai
authors.initials: F
authors.lastname: Liang
authors.forename: Rulin
authors.initials: R
authors.lastname: Liu
mesh_terms: D001407:Bacillus / Q000737:chemistry* / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001704:Biopolymers / Q000737:chemistry* / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005110:Extracellular Space / Q000737:chemistry* / Q000378:metabolism; D006358:Hot Temperature; D008969:Molecular Sequence Data; D010802:Phylogeny; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D001704:Biopolymers; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D011135:Polysaccharides, Bacterial; D012336:RNA, Ribosomal, 16S