title: Compartmentation of GABA B receptor2 expression in the mouse cerebellar cortex.
abstract: Despite the apparent uniformity in cellular composition of the adult mammalian cerebellar cortex, it is actually highly compartmentalized into transverse zones, and within each zone the cortex is further subdivided into a reproducible array of parasagittal stripes. The most extensively studied compartmentation antigen is zebrin II/aldolase c, which is expressed by a subset of Purkinje cells forming parasagittal stripes. Gamma-aminobutyric acid B receptors (GABABRs) are G-protein-coupled receptors that mediate a slow, prolonged form of inhibition in many brain areas. This study examines the localization of GABABR2 in the mouse cerebellum by using whole mount and section immunohistochemistry. The data reveal that GABABR2 immunoreactivity is expressed strongly in the dendrites of a subset of Purkinje cells that form a reproducible array of transverse zones and parasagittal stripes. By using double immunostaining, the striped pattern of GABABR2 expression was shown to be identical to that revealed by anti-zebrin II and complementary to that of phospholipase Cbeta4. This finding supports previous functional studies showing that inhibitory neurotransmission is highly patterned in the cerebellar cortex.
authors.affiliation: Department of Cell Biology & Anatomy, Hotchkiss Brain Institute, and Genes and Development Research Group, Faculty of Medicine, University of Calgary, Alberta, Canada.
authors.forename: Seung-Hyuk
authors.initials: SH
authors.lastname: Chung
authors.forename: Chul-Tae
authors.initials: CT
authors.lastname: Kim
authors.forename: Richard
authors.initials: R
authors.lastname: Hawkes
mesh_terms: D000818:Animals; D002525:Cerebellar Cortex / Q000502:physiology*; D003712:Dendrites / Q000502:physiology*; D007150:Immunohistochemistry; D051379:Mice; D011689:Purkinje Cells / Q000502:physiology*; D018080:Receptors, GABA-B / Q000235:genetics* / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology*
chemical_list: C485504:Gabbr2 protein, mouse; D018080:Receptors, GABA-B