Protein expression in the Drosophila Schneider 2 cell system.
The Schneider-2 (S2) Drosophila cell line is well suited for the stable overexpression of recombinant proteins using plasmid-based protein expression vectors. Following drug selection, a polyclonal S2 cell line can be induced to express on the order of 2 to 100 pmol/mg membrane protein for G-coupled protein receptors, 4000 to 100,000 sites/cell for other membrane receptors and 3 to 35 mg/liter for soluble and secreted proteins.
Schetz
John A
JA
University of North Texas Health Science Center, Fort Worth, Texas, USA.
Shankar
Eswar P N
EP
D000818:Animals; D035843:Biomedical Research / Q000379:methods*; D002460:Cell Line; D004330:Drosophila / Q000235:genetics / Q000378:metabolism*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D008565:Membrane Proteins / Q000378:metabolism; D009488:Neurosciences / Q000379:methods*; D010957:Plasmids; D011956:Receptors, Cell Surface / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism*; D015854:Up-Regulation
D008565:Membrane Proteins; D011956:Receptors, Cell Surface; D043562:Receptors, G-Protein-Coupled; D011994:Recombinant Proteins