[Genotype and sequence analysis on G2 segments of hantavirus from HFRS patients in Hebei Province].
OBJECTIVE
To know the genotype and subtype of hantavirus (HV) which infected persons in Hebei province.


METHODS
According to G2 coding region of 76-118 and R22 strains, specific type primers were designed to detect and identity the types of HV in HFRS patients' sera with RT-nested PCR. Nucleotides were assayed from partial products after purification and reclaim. Then, gene analysis was done with DNAStar package.


RESULTS
17 out of 69 positive serum specimens were successfully detected by RT-PCR and the detection rate was 24.64%, among which, <or= 7 days was 34.29%, 8-14 days was 19.23%, >or= 14 days were 0. 17 positive specimens were all belonged to SEO. The nucleotide homology of 9 typical specimens was 92.0%-100%. Between HeB7 and other 8 specimens was 92%-95%, and they belonged to different subtypes. When HeB7 compared with R22 strain, it was 97.7%. HeB7 and R22 belonged to S1 subtype. The 8 specimens except HeB7 was 95.7%-100% and they all belonged to S3 subtype. When compared with 76-118 strain, 9 specimens' nucleotide homology was only 70.3%-72.7%, belonged to different type.


CONCLUSIONS
SEO was the major type of HV from HFRS patients in Hebei province, S3 was the major subtype and S1 was also existed. In a certain area, the HV which belonged to the same type was correspondingly conservative, and had the characteristic of regional stability.
Li
Qi
Q
Hebei Province Center for Disease Control and Prevention, Shijiazhuang 050021, China. liqinew@yahoo.com.cn
Wei
Ya-mei
YM
Han
Zhan-ying
ZY
Zhang
Yan-bo
YB
Qi
Shun-xiang
SX
Xu
Yong-gang
YG
D002681:China; D005838:Genotype; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics*; D006480:Hemorrhagic Fever with Renal Syndrome / Q000175:diagnosis / Q000517:prevention & control / Q000628:therapy / Q000821:virology*; D006801:Humans; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014759:Viral Envelope Proteins / Q000235:genetics*
D014759:Viral Envelope Proteins; C090197:glycoprotein G2, Hantavirus