Chromatofocusing.
This unit discusses choice of chromatofocusing medium and buffers, determination of the quantity of medium needed, and preparation of CF buffers. A protocol is provided for a typical chromatofocusing experiment, and support protocols describe regeneration of the chromatofocusing column and several methods for separation of the polybuffer from the purified protein.
Williams
A
A
Pharmacia Biotech, Piscataway, New Jersey, USA.
D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D011506:Proteins