title: Bombyx mori nucleopolyhedrovirus orf25 encodes a 30kDa late protein in the infection cycle.
abstract: Bombyx mori nucleopolyhedrovirus (BmNPV) orf25 gene was characterized for the first time. The coding sequence of Bm25 was amplified and subcloned into the prokaryotic expression vector pGEX-4T-2 to produce glutathione S-transferase-tagged fusion protein in the BL21 (DE3) cells. The GST-Bm25 fusion protein was expressed efficiently after induction with IPTG. The purified fusion protein was used to immunize New Zealand white rabbits to prepare polyclonal antibody. Temporal expression analysis revealed a 30-kDa protein, which was detected beginning 24 hours post-infection using a polyclonal antibody against GST-Bm25 fusion protein. The transcript of Bm25 was detected by RT-PCR at 18-72 h p.i. In conclusion, the available data suggest that Bm25 encodes a 30kDa protein expressed in the late stage of infection cycle.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China. wanghaiyan2007@163.com
authors.forename: Haiyan
authors.initials: H
authors.lastname: Wang
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
authors.forename: Zhongjian
authors.initials: Z
authors.lastname: Guo
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000821:virology*; D015967:Gene Expression Regulation, Viral; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D008970:Molecular Weight; D017924:Nucleopolyhedroviruses / Q000737:chemistry / Q000235:genetics / Q000502:physiology*; D016366:Open Reading Frames; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D005982:Glutathione Transferase