[Inhomogeneous expression of fusion protein HSA/IL1ra in Pichia pastoris].
To investigate the causes and influencing factors of heterogeneity of HSA/IL1ra fusion protein expression in Pichia pastoris.



The heterogeneity of HSA/IL1ra fusion protein expressed in Pichia pastoris was studied by removing glycosylation and inhibiting glycosylation, as well as by different ways of fusion, different clones, and different expression host.



Glycosylation caused expression heterogeneity of fusion protein, but in SMD1168 and some GS115 clones there was no this phenomenon.



The expression heterogeneity of HSA/IL1ra fusion protein in Pichia pastoris is due to the glycosylation, and different ways of fusion, different clones, different expression host also have some impact.
Dai
Shou-feng
SF
Institution of Pharmacology & Toxicology and Biological Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Shen
Qi
Q
Chen
Jing
J
Chen
Shu-qing
SQ
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D018740:Genetic Heterogeneity; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D053590:Interleukin 1 Receptor Antagonist Protein / Q000096:biosynthesis* / Q000235:genetics; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics; D012709:Serum Albumin / Q000096:biosynthesis* / Q000235:genetics
D053590:Interleukin 1 Receptor Antagonist Protein; D011993:Recombinant Fusion Proteins; D012709:Serum Albumin