Development of molecular genetic interventions for HIV infection.
This unit includes a set of protocols for the ex vivo transfer of genes into CD4+ T cells, to be used in the initial evaluation of genes protecting against HIV infection in gene therapy protocols. The describes isolating and expanding CD4+ T cells from the patient. The cells are then transduced by either retroviral transduction or particle-mediated gene transfer and reinfused into the patient. To monitor the effectiveness of gene transfer, genomic DNA is prepared from the patient's cells. Detection of vector DNA by PCR analysis of the patient's genomic DNA following gene transfer is also described in detail.
Woffendin
C
C
University of Michigan Medical Center, Ann Arbor, Michigan, USA.
Ranga
U
U
Nabel
G J
GJ
D019470:Biolistics; D001804:Blood Transfusion, Autologous; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism / Q000637:transplantation; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors; D005826:Genetics, Medical; D015658:HIV Infections / Q000235:genetics / Q000628:therapy*; D006801:Humans; D066298:In Vitro Techniques; D016133:Polymerase Chain Reaction; D012190:Retroviridae / Q000235:genetics