One-dimensional electrophoresis using nondenaturing conditions.
Nondenaturing or "native" electrophoresis--i.e., electrophoresis in the absence of denaturants such as detergents and urea--is an often-overlooked technique for determining the native size, subunit structure, and optimal separation of a protein. Two protocols are presented in this unit: continuous PAGE, which is highly flexible, permitting cationic and anionic electrophoresis over a full range of pH, and discontinuous PAGE, which is limited to proteins negatively charged at neutral pH but provides high resolution for accurate size calibration.
Gallagher
S R
SR
Hoefer Pharmacia Biotech, San Francisco, California, USA.
D002021:Buffers; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D011489:Protein Denaturation; D012015:Reference Standards
D002021:Buffers