Construction and assay of radiation hybrids.
This unit describes the irradiation and fusion of cells to produce radiation hybrids and the harvesting and expansion of RH colonies. A support protocol describes amplification of human chromosome fragments by PCR and analysis of those fragments by agarose gel electrophoresis.
Richard
C W
CW
University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
Washington
S S
SS
D000818:Animals; D002459:Cell Fusion; D002877:Chromosomes, Human; D006224:Cricetinae; D004587:Electrophoresis, Agar Gel; D005819:Genetic Markers; D005826:Genetics, Medical; D006801:Humans; D016133:Polymerase Chain Reaction; D021101:Radiation Hybrid Mapping / Q000379:methods*
D005819:Genetic Markers