Engineering specific cross-links in nucleic acids using glycol linkers.
One of the most convenient methods for generating oligonucleotides possessing intra- or interstrand cross-links is through incorporation of oligoethylene glycol bridges by solid-phase synthesis. The reagents are commercially available or can be synthesized in a few easy synthetic steps. Unlike many other DNA and RNA cross-links, aspects of the structural and thermodynamic impact of modifying nucleic acids with oligoethylene glycols have been studied. This unit covers protection, phosphitylation, and preparation of the glycol linker for oligonucleotide synthesis.
O'Dea
T
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Boston College, Chestnut Hill, Massachusetts, USA.
McLaughlin
L W
LW
D001671:Biochemistry / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D019855:Ethylene Glycol / Q000737:chemistry; D006018:Glycols / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D009841:Oligonucleotides / Q000737:chemistry; D017905:Phosphites; D014320:Triphenylmethyl Compounds
D003432:Cross-Linking Reagents; D006018:Glycols; D009696:Nucleic Acids; D009841:Oligonucleotides; D017905:Phosphites; D014320:Triphenylmethyl Compounds; D019855:Ethylene Glycol