Concentrations of TATA box-binding protein (TBP)-type genes affect chordamesodermal gene expression.
The TATA box-binding protein (TBP) is an essential component of transcription initiation complexes of all three eukaryotic RNA polymerases. Recent knockdown studies revealed that basic transcription factors are essential not only for gene transcription but also for regulating specific gene expression. However, the mechanism of and the effect by regulation of TBP expression are unknown during early embryogenesis. Here we show that the alteration of concentration of each TBP-type gene affected mutually one another's expression, suggesting that an optimal ratio of concentrations of TBP-type genes induce expression of specific genes.
Goto
Toshiyasu
T
ICORP Organ Regeneration Project, Japan Science and Technology Agency, Tokyo, Japan.
Keller
Ray
R
Asashima
Makoto
M
D000818:Animals; D030801:Animals, Genetically Modified; D001483:Base Sequence; D018076:DNA, Complementary / Q000235:genetics; D018507:Gene Expression Regulation, Developmental; D018390:Gene Targeting; D049452:Green Fluorescent Proteins / Q000235:genetics; D009672:Notochord / Q000196:embryology*; D035181:TATA-Box Binding Protein / Q000235:genetics*; D029867:Xenopus Proteins / Q000235:genetics*; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics*
D018076:DNA, Complementary; D035181:TATA-Box Binding Protein; C492584:TBPL2 protein, Xenopus; D029867:Xenopus Proteins; D049452:Green Fluorescent Proteins