Purification of the eukaryotic 20S proteasome.
The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell. Combination of the 20S proteasome with a complex of regulatory proteins forms the 26S proteasome, which in turn is responsible for the recognition and degradation of ubiquitin-protein conjugates. As described in this unit, the constitutive form of the 20S proteasome can be conveniently purified as a stable and homogeneous preparation from bovine pituitaries. A support protocol details an enzyme assay used in evaluating proteasomal activity. The 20S proteasome is the catalytic core of the major extralysosomal proteolytic system of the cell.
Wilk
S
S
Mount Sinai School of Medicine, New York, New York, USA.
Chen
W E
WE
D000818:Animals; D002417:Cattle; D010902:Pituitary Gland / Q000201:enzymology; D046988:Proteasome Endopeptidase Complex / Q000302:isolation & purification* / Q000378:metabolism*
D046988:Proteasome Endopeptidase Complex