title: Presence, isolation and characterization of yolk DNA from chicken eggs.
abstract: Yolk DNA was detected with immunocytochemistry in fertilized chicken eggs. Yolk spheres were purifield with Ficoll-400 density gradient centrifugation, followed by extraction of DNA therein. Yolk DNA is sensitive to various restriction endonucleases. but shows no obvious bands, demonstrating its sequence heterogeneity. There is only a little, if any, methylated CpG in yolk DNA. judgmg from MspI and HpII digestion. Southern blot indicated that the sequence of yolk DNA accounts for a small portion of that of genomic DNA. Its uniqueness suggests that yolk DNA may play a crucial role in early development of chickens.
authors.affiliation: Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China.
authors.forename: S
authors.initials: S
authors.lastname: Lijun
authors.forename: Y
authors.initials: Y
authors.lastname: Lei
authors.forename: C
authors.initials: C
authors.lastname: Chuchu