Protection by polaprezinc against radiation-induced apoptosis in rat jejunal crypt cells.
Polaprezinc, an anti-ulcer drug, is a chelate compound consisting of zinc and L-carnosine. Polaprezinc has been shown to prevent gastric mucosal injury. The anti ulcer effects of polaprezinc have been ascribed to its antioxidative property. The effect of polaprezinc on ionizing radiation-induced apoptosis was studied in the jejunal epithelial crypt cells of rats. Seven-to eight week-old Wistar rats, which were treated with 100 mg/kg of polaprezinc orally 1h before irradiation or 2% carboxymethyl cellulose sodium in controls, were exposed to whole body X-ray irradiation at 2 Gy. The number of apoptotic cells per jejunum crypt was counted in haematoxylin and eosin stained sections at 0-6 h after irradiation. TUNEL positive cells and immunopositive cells for active caspase-3 per crypt were also counted. Accumulation of p53, p21(WAF1/CIP1) and Bax expression in the jejunum after irradiation were examined by Western blot analyses. Polaprezinc treatment given prior to radiation resulted in a significant reduction in numbers of apoptotic cells, TUNEL positive cells and active caspase-3 immunopositive cells in jejunal crypt cells. Polaprezinc treatment resulted in decreases of p53 accumulation, p21(WAF1/CIP1) and Bax expression after irradiation. Polaprezinc has a protective effect against ionizing radiation induced apoptosis in rat jejunal crypt cells.
Matsuu-Matsuyama
Mutsumi
M
Division of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University Graduate School of Biomedical Sciences, 1-12-4 Sakamoro, Nagasaki, Japan. mutsumi@net.nagasaki-u.ac.jp
Shichijo
Kazuko
K
Okaichi
Kumio
K
Nakayama
Toshiyuki
T
Nakashima
Masahiro
M
Uemura
Takashi
T
Niino
Daisuke
D
Sekine
Ichiro
I
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D002336:Carnosine / Q000008:administration & dosage / Q000031:analogs & derivatives*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D007583:Jejunum / Q000166:cytology* / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D008297:Male; D009942:Organometallic Compounds / Q000008:administration & dosage*; D011829:Radiation Dosage; D011837:Radiation-Protective Agents / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D017967:Zinc Compounds / Q000008:administration & dosage
D009942:Organometallic Compounds; D011837:Radiation-Protective Agents; D017967:Zinc Compounds; C061957:polaprezinc; D002336:Carnosine