[Expression and significance of nuclear factor-kappaB and ICAM-1 mRNA in nasal mucosa of allergic rhinitis].
OBJECTIVE
To clarify the pathogenesis of allergic rhinitis (AR) by detecting the activation of NF-kappaB and the expression of ICAM-1 mRNA in nasal mucosa of the patients with AR.


METHODS
AR patients were collected as experimental group and deflection of nasal septum patients as control group. Immunohistochemistry methods were used to examine the expression of NF-kappaB p50 and p65 subunits in the middle turbinate mucous tunic. ICAM-1 mRNA were detected by means of RT-PCR.


RESULTS
NF-kappaB was strongly expressed in AR group. NF-kappaB p50 existed in cytoplasm and nucleus. NF-kappaB p65 was mainly expressed in cytoplasm and less in nucleus. While NF-kappaB was expressed little in control group. The difference of NF-kappaB p50 and NF-kappaB p65 expression between two groups were significantly (P < 0.01). ICAM-1 mRNA was more strongly expressed in AR group (P < 0.01) while feebly expressed in deflection of nasal septum group. The expression of ICAM-1 mRNA were significantly correlated with that of NF-kappaB p50 and NF-kappaB p65 (r = 0.899 5, P < 0.01; r = 0.7601, P < 0.01).


CONCLUSIONS
NF-kappaB plays a key role in AR. The excessively activated NF-kappaB promotes the transcription of ICAM-1 mRNA. ICAM-1 relates to the pathogenesis and development of AR.
Zhao
Jiandong
J
Department of Otolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. zhaojd.jack@yahoo.com.cn
Wang
Yanjun
Y
Kong
Weijia
W
D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism*; D008297:Male; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D009297:Nasal Mucosa / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012220:Rhinitis / Q000378:metabolism* / Q000473:pathology; D051996:Transcription Factor RelA / Q000378:metabolism*
D052002:NF-kappa B p50 Subunit; C498143:RELA protein, human; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D018799:Intercellular Adhesion Molecule-1