title: WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells.
abstract: In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNFalpha treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNFalpha induced WDNM1 expression showed the NFkappaB-dependent mechanism since it's expression was abrogated in IkappaBalphaM (super-repressor of NFkappaB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNFalpha-NFkappaB signal pathway, is associated with HC11 cell apoptosis.
authors.affiliation: School of Agricultural and Biotechnology, College of Agriculture and Life Sciences Seoul National University, Seoul, South Korea.
authors.forename: Yoonjung
authors.initials: Y
authors.lastname: Kho
authors.forename: Sungchan
authors.initials: S
authors.lastname: Kim
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Yoon
authors.forename: Jai-Hee
authors.initials: JH
authors.lastname: Moon
authors.forename: Sungwook
authors.initials: S
authors.lastname: Kwak
authors.forename: Gyuman
authors.initials: G
authors.lastname: Park
authors.forename: Junghee
authors.initials: J
authors.lastname: Woo
authors.forename: Sejong
authors.initials: S
authors.lastname: Oh
authors.forename: Kichang
authors.initials: K
authors.lastname: Hong
authors.forename: Saehun
authors.initials: S
authors.lastname: Kim
authors.forename: Hyunggee
authors.initials: H
authors.lastname: Kim
authors.forename: Seungkwon
authors.initials: S
authors.lastname: You
authors.forename: Yunjaie
authors.initials: Y
authors.lastname: Choi
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology*; D053148:Caspase 3 / Q000378:metabolism; D053179:Caspase 7 / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D003001:Cloning, Molecular; D004815:Epidermal Growth Factor / Q000494:pharmacology; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008321:Mammary Glands, Animal / Q000166:cytology / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008856:Microscopy, Fluorescence; D016328:NF-kappa B / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D016328:NF-kappa B; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D004815:Epidermal Growth Factor; D053148:Caspase 3; D053179:Caspase 7