title: In vivo transfer of plasmid from food-grade transiting lactococci to murine epithelial cells.
abstract: We recently demonstrated that noninvasive food-grade Lactococcus lactis (L. lactis) can deliver eukaryotic expression plasmid in mammalian cells in vitro. Here, we evaluated, in vivo, whether a eukaryotic expression plasmid carried by lactococci can translocate to the epithelial cells of the intestinal membrane. The strain LL(pLIG:BLG1) carrying one plasmid containing a eukaryotic expression cassette encoding beta-lactoglobulin (BLG), a major allergen of cow's milk, was orally administered by gavage to mice. BLG cDNA was detected in the epithelial membrane of the small intestine of 40% of the mice and BLG was produced in 53% of the mice. Oral administration of LL(pLIG:BLG1) induced a low and transitory Th1-type immune response counteracting a Th2 response in case of further sensitization. We demonstrated for the first time the transfer of a functional plasmid to the epithelial membrane of the small intestine in mice by noninvasive food-grade lactococci.
authors.affiliation: Commissariat à l'Energie Atomique (CEA), Institut de Biologie et Technologies de Saclay (iBiTecS), Service de Pharmacologie et d'Immunologie, Gif-sur-Yvette, France. jean-marc.chatel@jouy.inra.fr
authors.forename: J-M
authors.initials: JM
authors.lastname: Chatel
authors.forename: L
authors.initials: L
authors.lastname: Pothelune
authors.forename: S
authors.initials: S
authors.lastname: Ah-Leung
authors.forename: G
authors.initials: G
authors.lastname: Corthier
authors.forename: J-M
authors.initials: JM
authors.lastname: Wal
authors.forename: P
authors.initials: P
authors.lastname: Langella
mesh_terms: D000485:Allergens / Q000235:genetics / Q000276:immunology; D000818:Animals; D018988:Bacterial Translocation; D002417:Cattle; D004847:Epithelial Cells / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D005260:Female; D015316:Genetic Therapy / Q000379:methods*; D007070:Immunoglobulin A / Q000032:analysis; D007073:Immunoglobulin E / Q000032:analysis; D007074:Immunoglobulin G / Q000032:analysis; D007421:Intestine, Small; D013294:Lactococcus lactis / Q000502:physiology*; D007782:Lactoglobulins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D016269:Milk Hypersensitivity / Q000276:immunology / Q000628:therapy*; D023421:Models, Animal; D010957:Plasmids; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
chemical_list: D000485:Allergens; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007782:Lactoglobulins; D007073:Immunoglobulin E