Existence of subtypes of gustducin-immunoreactive cells in the vallate taste bud of guinea pigs.
Vallate taste buds in the guinea-pig tongue were immunohistochemically investigated with regard to the colocalization of gustducin with calbindin-D28K (=spot 35 protein) and type III inositol triphosphate receptor (IP(3)R-3) in order to characterize gustducin-immunoreactive cells. Individual taste bud cells ranged from totally immunopositive to totally immunonegative for these three molecules. Among the immunoreactive cells, gustducin-immunoreactive cells were divided into two cell populations: one immunopositive and the other immunonegative for calbindin-D28K. Applying our previous data to the present results, the former cells should belong to Type III cells designated by electron microscopy. This finding provides new evidence regarding the taste bud types of cells expressing gustducin in the guinea pig.
Ohkubo
Yasuhiro
Y
Department of Histology, Nippon Dental University School of Life Dentistry at Niigata, Niigata, Japan.
Yokosuka
Hiroyuki
H
Kumakura
Masahiko
M
Yoshie
Sumio
S
D000818:Animals; D064092:Calbindin 1; D064026:Calbindins; D005260:Female; D006168:Guinea Pigs; D007150:Immunohistochemistry; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D008297:Male; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D013650:Taste Buds / Q000166:cytology* / Q000276:immunology / Q000378:metabolism*; D015293:Transducin / Q000276:immunology* / Q000378:metabolism*
C576500:Calb1 protein, rat; D064092:Calbindin 1; D064026:Calbindins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D064030:S100 Calcium Binding Protein G; C075192:gustducin; D015293:Transducin