The effect of a hyaluronan-carboxymethylcellulose membrane vs. polyglactin 910 mesh on intra-abdominal tumor formation in mice.
OBJECTIVE
Hyaluronan mediates growth of SW620 colon cancer cells. Because hyaluronan is the active ingredient in Seprafilm, we hypothesized that Seprafilm would affect intraperitoneal tumor growth in a mouse model of peritoneal seeding.


METHODS
Immunodeficient mice underwent laparotomy and intraperitoneal inoculation of 10(5) SW620 cells. Seprafilm (n = 22), Vicryl mesh (foreign body control; n = 24), or no material (sham; n = 19) was placed under the incision. Mice were killed after four weeks and tumors were dissected, counted, and weighed.


RESULTS
Ninety-five percent of mice in the sham group and 96 percent in the Vicryl group developed intraperitoneal tumors. In contrast, only 64 percent of mice in the Seprafilm group developed tumors (P = 0.024), and these tumors were smaller than those in the sham group; (Seprafilm = 42 +/- 9 mg vs. sham = 82 +/- 17 mg; P = 0.05). In contrast, tumors in the Vicryl group were dramatically larger (349 +/- 49 mg; P < 0.001 vs. sham or Seprafilm).


CONCLUSIONS
Despite previous data that suggested that hyaluronan increases colon cancer cell growth, we found that Seprafilm decreased tumor formation and tended to decrease size in this model. In contrast, Vicryl mesh increased tumor formation and size. Our results suggest that Seprafilm does not promote intraperitoneal tumor growth, especially compared with Vicryl mesh.
Lee
Peter K
PK
Department of Surgery, University of Minnesota, Minneapolis, Minnesota, USA.
Windsperger
Andrew P
AP
Wilson
Christopher M
CM
McCarthy
James B
JB
Wasiluk
Karen R
KR
Rothenberger
David A
DA
Bullard Dunn
Kelli M
KM
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D000818:Animals; D002266:Carboxymethylcellulose Sodium / Q000494:pharmacology; D005260:Female; D006820:Hyaluronic Acid / Q000494:pharmacology*; D016867:Immunocompromised Host; D008567:Membranes, Artificial; D051379:Mice; D016513:Mice, SCID; D023421:Models, Animal; D009366:Neoplasm Seeding; D010534:Peritoneal Neoplasms / Q000473:pathology*; D011098:Polyglactin 910 / Q000494:pharmacology*; D013526:Surgical Mesh; D014407:Tumor Cells, Cultured
D000276:Adjuvants, Immunologic; D008567:Membranes, Artificial; C118198:Seprafilm; D011098:Polyglactin 910; D006820:Hyaluronic Acid; D002266:Carboxymethylcellulose Sodium