Genetic analysis of phenylacetic acid catabolism in Arthrobacter oxydans CECT386.
Arthrobacter oxydans CECT386 is a Gram-positive bacterium able to use either phenylacetic acid or phenylacetaldehyde as the sole carbon and energy source for aerobic growth. Genes responsible for the catabolism of these compounds have been located at two chromosomal regions and were organized in one isolated paaN gene and two putative paa operons, one consisting of the paaD, paaF, tetR and prot genes, and one consisting of the paaG, paaH, paaI, paaJ, paaK and paaB genes. The identity of the paaF and paaN genes was supported by functional complementation experiments. A comparison with the paa catabolic genes and/or gene clusters of other bacteria that degrade these aromatic compounds is presented. The results of this study broaden the knowledge regarding the range of metabolic potential of this strain and eventually make it attractive for environmental applications.
Navarro-Llorens
Juana María
JM
Departamento de Bioquímica y Biología Molecular I, Universidad Complutense de Madrid, Ciudad Universitaria, s/n, 28040, Madrid, Spain.
Drzyzga
Oliver
O
Perera
Julián
J
D001173:Arthrobacter / Q000235:genetics* / Q000378:metabolism*; D002876:Chromosomes, Bacterial / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D005810:Multigene Family; D016366:Open Reading Frames; D009876:Operon; D010648:Phenylacetates / Q000378:metabolism*; D010957:Plasmids; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D010648:Phenylacetates; C025136:phenylacetic acid