Use of electrophilic substitution to form site-specific cross-links in DNA.
This unit describes the synthesis, purification, and analysis of modified oligodeoxynucleotides containing 4-thio-2'-deoxyuridine, and the use of these modified oligodeoxynucleotides in conjunction with bis-electrophilic tethers to form site-specific cross-links with a complementary DNA target. These modified oligodeoxynucleotides provide a flexible tool for studies directed at biophysical investigation of oligonucleotide structure, as well as investigation of DNA hybridization, stability, and sequence-dependent base nucleophilicity.
Pires
R M
RM
The Ohio State University, Columbus, Ohio, USA.
Coleman
R S
RS
D001671:Biochemistry / Q000379:methods*; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D010655:Phenylenediamines / Q000737:chemistry
D003432:Cross-Linking Reagents; D010655:Phenylenediamines; D004247:DNA