Alternative pathway evaluation.
The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.
Giclas
P C
PC
National Jewish Center for Immunology and Respiratory Medicine, Denver, Colorado, USA.
D000818:Animals; D011415:Complement Factor B / Q000032:analysis; D011416:Complement Factor D / Q000032:analysis; D015941:Complement Hemolytic Activity Assay / Q000379:methods*; D003170:Complement Pathway, Alternative; D004912:Erythrocytes / Q000276:immunology; D006801:Humans; D016014:Linear Models; D011336:Probability; D011414:Properdin / Q000032:analysis; D011817:Rabbits; D012680:Sensitivity and Specificity
D011414:Properdin; D011416:Complement Factor D; D011415:Complement Factor B