Isolation of murine hematopoietic stem cells and progenitor cells.
Protocols for the purification of CD34-KSL cells, CD34+KSL cells, SP cells, and Tip-SP CD34-KSL cells are described. Mouse hematopoietic stem cells are highly enriched in the CD34-KSL and SP fractions. These two populations overlap one another. A nearly homogenous population of HSCs can be obtained by isolating Tip-SP CD34-KSL cells. One of the earliest progenitor cell populations is CD34+KSL cells.
Ema
Hideo
H
The University of Tokyo, Tokyo, Japan.
Morita
Yohei
Y
Nakauchi
Hiromitsu
H
Matsuzaki
Yumi
Y
D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology*; D019070:Cell Lineage; D002469:Cell Separation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL
D018952:Antigens, CD34