title: Induction of neural stem cell-like cells (NSCLCs) from mouse astrocytes by Bmi1.
abstract: Recently, Bmi1 was shown to control the proliferation and self-renewal of neural stem cells (NSCs). In this study, we demonstrated the induction of NSC-like cells (NSCLCs) from mouse astrocytes by Bmi1 under NSC culture conditions. These NSCLCs exhibited the morphology and growth properties of NSCs, and expressed NSC marker genes, including nestin, CD133, and Sox2. In vitro differentiation of NSCLCs resulted in differentiated cell populations containing astrocytes, neurons, and oligodendrocytes. Following treatment with histone deacetylase inhibitors (trichostatin A and valproic acid), the potential of NSCLCs for proliferation, dedifferentiation, and self-renewal was significantly inhibited. Our data indicate that multipotent NSCLCs can be generated directly from astrocytes by the addition of Bmi1.
authors.affiliation: Laboratory of Cell Growth and Function Regulation, College of Life Sciences and Biotechnology, Korea University, 5-1, Anam-Dong, Sungbuk-ku, Seoul 136-701, Republic of Korea.
authors.forename: Jai-Hee
authors.initials: JH
authors.lastname: Moon
authors.forename: Byung Sun
authors.initials: BS
authors.lastname: Yoon
authors.forename: Bona
authors.initials: B
authors.lastname: Kim
authors.forename: Gyuman
authors.initials: G
authors.lastname: Park
authors.forename: Hye-Youn
authors.initials: HY
authors.lastname: Jung
authors.forename: Isaac
authors.initials: I
authors.lastname: Maeng
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Jun
authors.forename: Seung Jun
authors.initials: SJ
authors.lastname: Yoo
authors.forename: Aeree
authors.initials: A
authors.lastname: Kim
authors.forename: Sejong
authors.initials: S
authors.lastname: Oh
authors.forename: Kwang Youn
authors.initials: KY
authors.lastname: Whang
authors.forename: Hyunggee
authors.initials: H
authors.lastname: Kim
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Kim
authors.forename: Ki Dong
authors.initials: KD
authors.lastname: Kim
authors.forename: Seungkwon
authors.initials: S
authors.lastname: You
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D015703:Antigens, CD / Q000235:genetics; D001253:Astrocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D019070:Cell Lineage / Q000235:genetics; D049109:Cell Proliferation; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics; D005819:Genetic Markers; D006023:Glycoproteins / Q000235:genetics; D024002:HMGB Proteins / Q000235:genetics; D007381:Intermediate Filament Proteins / Q000235:genetics; D051379:Mice; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics; D064231:Nestin; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000502:physiology*; D010455:Peptides / Q000235:genetics; D063150:Polycomb Repressive Complex 1; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000502:physiology*; D012097:Repressor Proteins / Q000235:genetics / Q000502:physiology*; D055748:SOXB1 Transcription Factors; D014157:Transcription Factors / Q000235:genetics
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; C489421:Bmi1 protein, mouse; C417743:Cdkn2a protein, mouse; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004268:DNA-Binding Proteins; D005819:Genetic Markers; D006023:Glycoproteins; D024002:HMGB Proteins; D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D009687:Nuclear Proteins; D010455:Peptides; C000606024:Prom1 protein, mouse; D047428:Protein Kinase Inhibitors; D011518:Proto-Oncogene Proteins; D012097:Repressor Proteins; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D014157:Transcription Factors; D063150:Polycomb Repressive Complex 1