title: The identity of the transcription +1 position is crucial for changes in gene expression in response to amino acid starvation in Bacillus subtilis.
abstract: We identify here a pattern in the transcription start sites (+1A or +1G) of sigma(A)-dependent promoters of genes that are up-/downregulated in response to amino acid starvation (stringent response) in Bacillus subtilis. Upregulated promoters initiate mostly with ATP and downregulated promoters with GTP. These promoters appear to be sensitive to changes in initiating nucleoside triphosphate concentrations. During the stringent response in B. subtilis, when ATP and GTP levels change reciprocally, the identity of the +1 position (A or G) of these promoters is a factor important in their regulation. Mutations that change the identity of position +1 (A for G and vice versa) change the response of the promoter to amino acid starvation.
authors.affiliation: Laboratory of Molecular Genetics of Bacteria, Institute of Microbiology ASCR, Vídenská 1083, Prague 142 20, Czech Republic. krasny@img.cas.cz
authors.forename: Libor
authors.initials: L
authors.lastname: Krásný
authors.forename: Hana
authors.initials: H
authors.lastname: Tiserová
authors.forename: Jirí
authors.initials: J
authors.lastname: Jonák
authors.forename: Dominik
authors.initials: D
authors.lastname: Rejman
authors.forename: Hana
authors.initials: H
authors.lastname: Sanderová
mesh_terms: D000596:Amino Acids / Q000378:metabolism*; D001412:Bacillus subtilis / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D009711:Nucleotides / Q000378:metabolism; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins; D009711:Nucleotides