title: Experimental asexual blood stage malaria immunity.
abstract: This unit describes three methods for investigating the immune response to murine malaria parasites. Immunization protocols using recombinant fragments of the merozoite surface protein-1 of Plasmodium yoelii, whole blood stage malaria parasites, and live infection with parasitized red blood cells from a Plasmodium-infected donor are provided. Methods for chemotherapeutic drug care of Plasmodium-infected mice and for inducing malaria by adoptive transfer of antigen-specific T cells are included. Finally, support protocols describe methods for growing, maintaining, and cryopreserving murine asexual blood stage malaria parasites and for preparing blood stage antigen for use in ELISAs.
authors.affiliation: The Queensland Institute of Medical Research, Queensland, Australia.
authors.forename: F H
authors.initials: FH
authors.lastname: Amante
authors.forename: M F
authors.initials: MF
authors.lastname: Good
mesh_terms: D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology; D015925:Cryopreservation; D004912:Erythrocytes / Q000276:immunology / Q000469:parasitology; D006801:Humans; D007114:Immunization; D008288:Malaria / Q000276:immunology* / Q000628:therapy*; D017780:Malaria Vaccines / Q000276:immunology*; D020066:Merozoite Surface Protein 1 / Q000276:immunology; D051379:Mice; D010967:Plasmodium yoelii / Q000254:growth & development / Q000276:immunology; D011994:Recombinant Proteins / Q000276:immunology; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000953:Antigens, Protozoan; D017780:Malaria Vaccines; D020066:Merozoite Surface Protein 1; D011994:Recombinant Proteins