Quality control assessment of human immunodeficiency virus type 2 (HIV-2) viral load quantification assays: results from an international collaboration on HIV-2 infection in 2006.
Human immunodeficiency virus type 2 (HIV-2) RNA quantification assays used in nine laboratories of the ACHI(E)V(2E) (A Collaboration on HIV-2 Infection) study group were evaluated. In a blinded experimental design, laboratories quantified three series of aliquots of an HIV-2 subtype A strain, each at a different theoretical viral load. Quantification varied between laboratories, and international standardization of quantification assays is strongly needed.
Damond
Florence
F
APHP, Hôpital Bichat-Claude Bernard, Laboratoire de Virologie, Paris, 75018 France. florence.damond@bch.aphp.fr
Benard
Antoine
A
Ruelle
Jean
J
Alabi
Abraham
A
Kupfer
Bernd
B
Gomes
Perpetua
P
Rodes
Berta
B
Albert
Jan
J
Böni
Jürg
J
Garson
Jeremy
J
Ferns
Bridget
B
Matheron
Sophie
S
Chene
Geneviève
G
Brun-Vezinet
Françoise
F
D003299:Cooperative Behavior; D015658:HIV Infections / Q000821:virology*; D015498:HIV-2 / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D038622:Internationality; D007753:Laboratories / Q000592:standards; D011786:Quality Control; D012367:RNA, Viral / Q000097:blood / Q000592:standards*; D011933:Reagent Kits, Diagnostic / Q000592:standards*; D015203:Reproducibility of Results; D019562:Viral Load / Q000592:standards*
D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic