Synthesis of oligonucleotide conjugates via aqueous Diels-Alder cycloaddition.
The conjugation of maleimide reporter groups to 5'-diene-modified oligonucleotides via aqueous Diels-Alder cycloaddition is described. Detailed basic protocols are provided for the preparation of a diene-amidite (5'-diene modifier), for the attachment of a diene group to the 5'-terminus of oligonucleotides, and for the conjugation of maleimide reporter groups to diene-modified oligonucleotides.
Leuck
Michael
M
Proligo LLC, Boulder, Colorado, USA.
Wolter
Andreas
A
D008301:Maleimides / Q000737:chemistry*; D008956:Models, Chemical; D015394:Molecular Structure; D009841:Oligonucleotides / Q000737:chemistry*; D020135:Peptide Nucleic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D008301:Maleimides; D009841:Oligonucleotides; D020135:Peptide Nucleic Acids; D014867:Water; C043592:maleimide