title: Identification of a porcine calicivirus related genetically to human sapoviruses.
abstract: Whether animals may act as reservoirs for human caliciviruses is unclear. By sequence analysis of a short fragment of the RNA-dependent RNA polymerase (RdRp) region, porcine sapovirus (SaV) strains that genetically resemble human SaVs have been detected in piglets, but more-informative sequences (capsid gene) were not available for a precise characterization. In this study, the 3' terminus (the 3' end of open reading frame 1 [ORF1], including the polymerase complex and the complete capsid; ORF2; and the 3' untranslated region) of one such human SaV-like strain, 43/06-18p3/2006/It, was determined, revealing that these viruses are more related genetically to human (47.4 to 54.9% amino acid identity) than to animal (35.2 to 44.7% amino acid identity) SaVs in the capsid gene. In addition, the recombination-prone RdRp-capsid junction region was highly conserved with those of human SaVs of genogroup GI. The presence of porcine viruses similar to human SaVs is a significant finding because of the potential for zoonotic infections or generation of porcine/human recombinants.
authors.affiliation: Dipartimento di Sanità Pubblica e Zootecnia, Facoltà di Medicina Veterinaria di Bari, S.p. per Casamassima Km 3, 70010 Valenzano, Bari, Italy. v.martella@veterinaria.uniba.it
authors.forename: V
authors.initials: V
authors.lastname: Martella
authors.forename: E
authors.initials: E
authors.lastname: Lorusso
authors.forename: K
authors.initials: K
authors.lastname: Banyai
authors.forename: N
authors.initials: N
authors.lastname: Decaro
authors.forename: M
authors.initials: M
authors.lastname: Corrente
authors.forename: G
authors.initials: G
authors.lastname: Elia
authors.forename: A
authors.initials: A
authors.lastname: Cavalli
authors.forename: A
authors.initials: A
authors.lastname: Radogna
authors.forename: V
authors.initials: V
authors.lastname: Costantini
authors.forename: L J
authors.initials: LJ
authors.lastname: Saif
authors.forename: A
authors.initials: A
authors.lastname: Lavazza
authors.forename: L
authors.initials: L
authors.lastname: Di Trani
authors.forename: C
authors.initials: C
authors.lastname: Buonavoglia
mesh_terms: D000818:Animals; D001483:Base Sequence; D002139:Caliciviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017250:Caliciviridae Infections / Q000662:veterinary / Q000821:virology*; D002213:Capsid / Q000737:chemistry; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000662:veterinary / Q000821:virology; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D029341:Sapovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology*
chemical_list: D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase