title: A new method for the mapping of 5' ends of RNAs.
abstract: In this article, we describe a new procedure to map 5' ends of RNAs. The procedure consists in the use of specific RNase H digestion of a hybrid formed by the RNA and a complementary DNA oligonucleotide. Northern blot hybridization of the resulting RNA fragment allows an accurate measurement of its length. Although we generally use this procedure as a control of previously performed primer extension analyses, the absence of nonspecific bands, which often occur in primer extensions on RNA templates with extended secondary structures, suggests that our method may be preferable when these difficult templates are analyzed.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, Palermo 90128, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Bellavia
authors.forename: Giusi Irma
authors.initials: GI
authors.lastname: Forte
authors.forename: Giorgia
authors.initials: G
authors.lastname: Sisino
authors.forename: Giada
authors.initials: G
authors.lastname: Sisino
authors.forename: Rainer
authors.initials: R
authors.lastname: Barbieri
mesh_terms: D000818:Animals; D015152:Blotting, Northern / Q000379:methods*; D012313:RNA / Q000032:analysis* / Q000235:genetics* / Q000378:metabolism; D012335:RNA, Ribosomal / Q000032:analysis / Q000235:genetics; D016914:Ribonuclease H / Q000378:metabolism; D012617:Sea Urchins / Q000235:genetics; D013698:Templates, Genetic
chemical_list: D012335:RNA, Ribosomal; D012313:RNA; D016914:Ribonuclease H