Characterisation of the first complete genome sequence of the roe deer (Capreolus capreolus) papillomavirus.
The complete genomic DNA of a novel roe deer (Capreolus capreolus) papillomavirus (CcPV1) was amplified and sequenced from fibropapillomatous skin lesions of a Hungarian roe deer. Viral DNA was detected by a pair of degenerate primers and the remaining genomic sequence was amplified by a long-template high-fidelity PCR and sequenced. The CcPV1 genome was 8032 bp long and contained open reading frames (ORFs) typical for Delta-papillomaviruses (E6, E7, E1, E2, E4, E5, E9, L2, and L1) and a 799 bp long untranslated regulatory region (URR). Phylogenetic analysis based on the 3861 bp long concatenated sequence of the E1-E2-L2-L1 ORFs and on separate alignments of all major ORFs using both neighbour-joining and maximum parsimony methods placed CcPV1 on a distinct branch between Ovine papillomavirus 1 and the other deer papillomaviruses within the Delta-papillomavirus genus, although pairwise nucleotide alignments of L1 ORF sequences determined highest identities with European Elk Papillomavirus (71.2%) and Reindeer Papillomavirus (70.3%).
Erdélyi
Károly
K
Central Agriculture Office, Veterinary Diagnostic Directorate, Tábornok u. 2, H-1149 Budapest, Hungary. erdelyi@oai.hu
Bálint
Adám
A
Dencso
László
L
Dán
Adám
A
Ursu
Krisztina
K
D000818:Animals; D004279:DNA, Viral / Q000032:analysis; D003670:Deer / Q000821:virology*; D052687:Deltapapillomavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D016679:Genome, Viral; D007636:Keratoacanthoma / Q000662:veterinary* / Q000821:virology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D030361:Papillomavirus Infections / Q000662:veterinary* / Q000821:virology; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D012878:Skin Neoplasms / Q000662:veterinary* / Q000821:virology; D014764:Viral Proteins / Q000235:genetics
D004279:DNA, Viral; D014764:Viral Proteins