Introduction to the synthesis and purification of oligonucleotides.
Modern nucleic acid synthesizers utilize phosphite triester chemistries that employ stable phosphoramidite monomers to build a growing polymer. These robust reactions allow easy generation of specific oligodeoxyribo- and oligoribonucleotides with a variety of labels, modified linkages, and nonstandard bases. Strategies are given for the maximization of synthetic yield, the generation of sequences containing site-specific modifications, and the isolation of synthetic oligonucleotides. Protocols describe monitoring the progress of synthesis via the trityl assay and methods for deprotection.
Ellington
A
A
University of Texas, Austin, Texas, USA.
Pollard
J D
JD
D001671:Biochemistry / Q000379:methods*; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry; D007202:Indicators and Reagents; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification*; D009943:Organophosphorus Compounds / Q000737:chemistry; D012313:RNA / Q000138:chemical synthesis / Q000737:chemistry; D012996:Solutions
D007202:Indicators and Reagents; D009841:Oligonucleotides; D009943:Organophosphorus Compounds; D012996:Solutions; C434331:phosphoramidite; D012313:RNA; D004247:DNA