[Analysis on expression and molecular basis for ABO glycosyltransferase with dual specificity].
In order to elucidate the expression and molecular genetic background of ABO gene seven samples with ABO discrepancy further identified as bi-specific ABO gene were studied. All these samples were subjected to phenotyping by monoclonal and polyclonal antisera and were then genotyped by direct DNA sequencing and haplotype-sequencing at the exon 6 and 7 of ABO gene. As a result, six ABO dual-specific alleles were identified in Chinese population. An antigen expressed by these B (A) or Cis-AB individuals varied from very low level to the normal level, compared with common A blood group samples. In conclusion, molecular genetic backgrounds of two pairs out of four samples in all samples were the same, however, the ABO expression showed diverse.
Deng
Zhi-Hui
ZH
Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China. zhihui_deng@yahoo.com.cn
Zhang
Hao
H
Zeng
Jian-Qiang
JQ
Yu
Qiong
Q
Su
Yu-Qing
YQ
Liang
Yan-Lian
YL
Li
Qian
Q
D000017:ABO Blood-Group System / Q000235:genetics*; D044466:Asian People / Q000235:genetics; D004252:DNA Mutational Analysis; D004912:Erythrocytes / Q000166:cytology / Q000201:enzymology / Q000378:metabolism*; D005091:Exons / Q000235:genetics; D016695:Glycosyltransferases / Q000737:chemistry / Q000235:genetics*; D006801:Humans
D000017:ABO Blood-Group System; D016695:Glycosyltransferases