Synthesis of multiple peptides on plastic pins.
This unit covers the strategy of the multiple peptide approach to biological scanning, the synthetic protocols, and the handling of peptides after synthesis: cleavage, preliminary purification, storage, and analysis. It is specific for the multipin technique using equipment obtained from Chiron Technologies, although some of the approaches are applicable to other multiple synthesis techniques. This unit also includes protocols for preparing Fmoc-amino acid solutions and for acetylating or biotinylating synthesized peptides.
Rodda
S J
SJ
Chiron Technologies Pty. Ltd., Victoria, Australia.
D000107:Acetylation; D019921:Biotinylation; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry* / Q000302:isolation & purification; D010969:Plastics; D013672:Technology / Q000295:instrumentation / Q000379:methods
D010455:Peptides; D010969:Plastics