Molecular characterization of multiple xylanase producing thermophilic/thermotolerant fungi isolated from composting materials.
OBJECTIVE
Molecular characterization of commercially important group of xylanase producing thermophilic/thermotolerant fungi.


RESULTS
DNA from 16 thermophilic/thermotolerant fungal isolates was amplified by PCR using three sets of primers: (i) internal transcribed spacer sequence (ITSI-5.8S-ITSII), (ii) D1/D2 hyper variable region of 26S rDNA and (iii) 18S rDNA region. The amplified products of internal transcribed spacers (ITS) and D1/D2 region were sequenced and analysed using CLUSTALX, whereas, amplified 18S rDNA region was subjected to RFLP analysis based on restriction digestion with RsaI, MboI and Hinf I.


CONCLUSIONS
The sequence based analyses of ITSI-5.8S-ITSII as compared with D1/D2 region of 26-28S rDNA was found to be a better tool for phylogenetic resolution of thermophilic/thermotolerant fungi. The ITSI-5.8S-ITSII sequence-based dendrogram indicates an early divergence of the alkaline active xylanase producing thermophilic fungal strains.


CONCLUSIONS
This study was the first report on phylogenetic characterization of thermophilic/thermotolerant fungi.
Sharma
M
M
Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, India.
Chadha
B S
BS
Kaur
M
M
Ghatora
S K
SK
Saini
H S
HS
D004271:DNA, Fungal / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005658:Fungi / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016533:Mycological Typing Techniques; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D015183:Restriction Mapping; D012988:Soil Microbiology; D013696:Temperature
D004271:DNA, Fungal; D004275:DNA, Ribosomal; D012337:RNA, Ribosomal, 18S; D043364:Endo-1,4-beta Xylanases