title: Concentration and recovery of coliphages from water with bituminous coal.
abstract: Coliphages represent a process indicator for fecal pollution. The coal bed concentration method prepared for enterovirus was refined for a concentration of coliphages. A bed made from 1.5 g of 120-mesh coal powder was used for concentrating coliphage from 200 mL of a water sample with or without the addition of aluminum chloride at different pH values. The isolated E. coli strain EC-R8 was found to be more susceptible to the desired coliphage and showed significant coliphage-coliform response, with clear plaque used for further studies. The complete coliphage adsorption was achieved with the addition of 0.0005 M AlCl3 at pH 6.0. Adsorbed coliphages were eluted with 3% beef extract in Mcllvaine buffer at pH 7.1, with an average recovery of 78.74%. This concentration technique was applied for the detection of coliphages from the well water of Nagpur city (India) and found to contain coliphages in the range of 2 to 28 plaque-forming units per liter (PFU/L).
authors.affiliation: National Environmental Engineering Research Institute, Nagpur, India.
authors.forename: Nishant
authors.initials: N
authors.lastname: Dafale
authors.forename: Shrikumar
authors.initials: S
authors.lastname: Lakhe
authors.forename: Krishnakant
authors.initials: K
authors.lastname: Yadav
authors.forename: Hemant
authors.initials: H
authors.lastname: Purohit
authors.forename: Tapan
authors.initials: T
authors.lastname: Chakrabarti
mesh_terms: D000327:Adsorption; D003031:Coal; D003090:Coliphages / Q000302:isolation & purification*; D005374:Filtration / Q000379:methods*; D006801:Humans; D014876:Water Pollution / Q000032:analysis*; D018508:Water Purification / Q000379:methods; D014881:Water Supply / Q000032:analysis
chemical_list: D003031:Coal