title: Measurement of prostaglandins and other eicosanoids.
abstract: Eicosanoids are arachidonic acid metabolites formed through the cyclooxygenase or one of the lipoxygenase metabolic pathways. They include the prostaglandins (PG), leukotrienes (LT), thromboxanes (Tx), peptidoleukotrienes, and 5-hydroxyeicosatetraenoic acid (5-HETE). This unit describes procedures for measuring individual eicosanoids using several commercial kits. These include kits for an enzyme immunoasssay (ELISA) and a radioimmunoassay (RIA). A method is also described that can be used to analyze the full range of eicosanoids produced in a biological system, and a support protocol presents an acetone/chloroform extraction method for purifying eicosanoids prior to assay. Another support protocol describes a different extraction protocol in which samples are run over an octadecylsilyl silica column.
authors.affiliation: Washington University School of Medicine, St. Louis, Missouri, USA.
authors.forename: W F
authors.initials: WF
authors.lastname: Stenson
mesh_terms: D000818:Animals; D015777:Eicosanoids / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D011453:Prostaglandins / Q000032:analysis*; D011863:Radioimmunoassay / Q000379:methods*; D011933:Reagent Kits, Diagnostic
chemical_list: D015777:Eicosanoids; D011453:Prostaglandins; D011933:Reagent Kits, Diagnostic