title: Studying gene function in Caenorhabditis elegans using RNA-mediated interference.
abstract: The RNA interference (RNAi) method for targeted gene silencing is widely used in Caenorhabditis elegans for large-scale functional genomic studies, analysis of limited gene sets and detailed analysis of individual gene function. The application of RNAi has identified genes that participate in various aspects of development, physiology and cell biology. In addition, RNAi has been used to identify interacting genes and to study functionally redundant genes. This review discusses the various applications of RNAi in C. elegans, focusing particularly on the analysis of developmental processes.
authors.affiliation: Department of Biology, Syracuse University, Syracuse, NY 13244, USA. emmaine@syr.edu
authors.forename: Eleanor M
authors.initials: EM
authors.lastname: Maine
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000235:genetics* / Q000502:physiology; D029742:Caenorhabditis elegans Proteins / Q000235:genetics* / Q000502:physiology; D023281:Genomics / Q000379:methods; D034622:RNA Interference
chemical_list: D029742:Caenorhabditis elegans Proteins