Introduction of plasmid DNA into cells.
This appendix presents a procedure for transformation using calcium chloride, and also an alternate procedure for one-step preparation and transformation of competent cells.
Seidman
C E
CE
Harvard Medical School, Boston, Massachusetts, USA.
Struhl
K
K
D002122:Calcium Chloride; D004269:DNA, Bacterial / Q000235:genetics*; D004274:DNA, Recombinant / Q000235:genetics*; D004926:Escherichia coli / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics*; D010957:Plasmids / Q000235:genetics*; D014169:Transformation, Bacterial
D004269:DNA, Bacterial; D004274:DNA, Recombinant; D002122:Calcium Chloride