Characterization of variable genomic regions of Indian white spot syndrome virus.
A recent study examining genetic variations among the 3 completely sequenced white spot syndrome virus (WSSV) genomes isolated from China, Thailand and Taiwan revealed five major differences among them. Of these differences, a deletion region between ORF 23/24 and a variable region of ORF14/15 prone to recombination were of particular evolutionary significance. Focusing on these regions, 81 WSSV isolates from India were characterized by sequencing polymerase chain reaction (PCR) amplicons. The Indian strains carried a 10,970 bp deletion in the ORF 23/24 region relative to WSSV-TW and WSSV-TH-96-II. Analysis of the ORF 14/15 regions revealed two novel strains of WSSV with unique sequences which could have evolved by recombination. None of the WSSV isolates had a transposase sequence or VP35 gene as reported for Taiwan isolates. The Indian strains were closely related to Thailand strains suggesting movement of a putative ancestor from Thailand to other parts of the world including India.
Pradeep
Balakrishnan
B
Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences University, College of Fisheries, Mangalore-575 002, India.
Shekar
Malathi
M
Karunasagar
Iddya
I
Karunasagar
Indrani
I
D000818:Animals; D004279:DNA, Viral / Q000235:genetics*; D019143:Evolution, Molecular; D005814:Genes, Viral; D016679:Genome, Viral; D007194:India; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D033561:Penaeidae / Q000821:virology; D011110:Polymorphism, Genetic; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017384:Sequence Deletion; D026801:Synteny; D046848:White spot syndrome virus 1 / Q000235:genetics* / Q000302:isolation & purification
D004279:DNA, Viral