Isolation and purification of C3 from human plasma.
The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.
O'Rear
Lynda D
LD
University of Louisville, Louisville, Kentucky.
Ross
Gordon D
GD
University of Louisville, Louisville, Kentucky.
D002853:Chromatography, Liquid / Q000379:methods; D003176:Complement C3 / Q000737:chemistry / Q000302:isolation & purification*; D003182:Complement C5 / Q000032:analysis; D015941:Complement Hemolytic Activity Assay / Q000379:methods; D004912:Erythrocytes / Q000276:immunology; D004983:Ethanolamines / Q000737:chemistry; D006801:Humans; D008239:Lysine / Q000737:chemistry; D010949:Plasma / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000737:chemistry
D003176:Complement C3; D003182:Complement C5; D004983:Ethanolamines; D012685:Sepharose; D008239:Lysine; C007369:2-diethylaminoethanol