Probing nucleic acid structure with nickel- and cobalt-based reagents.
The use of nickel and cobalt reagents is presented for characterizing the solvent exposure of guanine residues in DNA and RNA. These reagents promote guanine oxidation in the presence of a peracid such as monopersulfate, and the extent of reaction indicates the steric and electronic environment surrounding the N7 and aromatic face of this residue. Since oxidation does not itself perturb target structure or induce strand scission, it is coupled with fragmentation by treatment with piperidine (for smaller polynucleotides) or termination of primer extension (for larger polynucleotides).
Rokita
S E
SE
University of Maryland, College Park, Maryland, USA.
Burrows
C J
CJ
D001671:Biochemistry / Q000379:methods*; D003035:Cobalt / Q000737:chemistry*; D004247:DNA / Q000737:chemistry; D006147:Guanine / Q000737:chemistry; D007202:Indicators and Reagents; D009532:Nickel / Q000737:chemistry*; D009696:Nucleic Acids / Q000737:chemistry*; D010084:Oxidation-Reduction; D010880:Piperidines / Q000737:chemistry
D007202:Indicators and Reagents; D009696:Nucleic Acids; D010880:Piperidines; D003035:Cobalt; D006147:Guanine; C032727:piperidine; D009532:Nickel; D004247:DNA