title: Inhibitory effects of bovine lactoferrin and lactoferricin B on Enterobacter sakazakii.
abstract: The susceptibility of Enterobacter sakazakii, a food-borne pathogen, to several metal-bound forms of bovine lactoferrin (LF), pepsin-hydrolyzed LF (LF-hyd), and LF-derived peptide lactoferricin B (LFcin B) was tested. MIC and MBC testing revealed that 4 strains of E. sakazakii show susceptibility to apo- and Cu-LF, LF-hyd, and LFcin B, but not to Fe-LF, similarly to Escherichia coli. A growth curve test indicated that E. sakazakii was inhibited in a dose-dependent manner by apo-LF at 0.5 to 8 mg/ml. Even after being heated at 80 degrees C, LF at above 1 mg/ml inhibited the bacterial growth. These results suggest that bovine LF-related compounds may be useful for the inhibition of E. sakazakii in foods.
authors.affiliation: Food Science & Technology Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa 228-8583, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Wakabayashi
authors.forename: Koji
authors.initials: K
authors.lastname: Yamauchi
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Takase
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D044083:Cronobacter sakazakii / Q000187:drug effects* / Q000254:growth & development; D007781:Lactoferrin / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; C082171:lactoferricin B; D007781:Lactoferrin