Preparation of DNA from fixed, paraffin-embedded tissue.
Purification of DNA from fixed, paraffin-embedded tissue (PET) requires the removal of paraffin, proteins, and, in some cases, heavy metal ions. The resulting DNA is not generally suitable for Southern blot analysis. However, the polymerase chain reaction (PCR) can be used to amplify short regions of this DNA. The Basic Protocol is rapid and involves only a small number of manipulations, thus minimizing the possibility of contamination. The Alternate Protocol involves additional steps including extractions with xylenes and phenol/chloroform. This procedure is recommended as the primary approach when tissue has not been fixed in an optimal manner or as a secondary approach when the Basic Protocol has failed to produce satisfactory DNA.
Fox
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righam and Women's Hospital, Boston, Massachusetts, USA.
D004247:DNA / Q000235:genetics / Q000302:isolation & purification*; D005821:Genetic Techniques; D005826:Genetics, Medical; D006801:Humans; D016612:Paraffin Embedding; D016707:Tissue Fixation
D004247:DNA