title: Regulation of mitochondrial dynamics--characterization of fusion and fission genes in the ascomycete Podospora anserina.
abstract: The filamentous ascomycete Podospora anserina is a model system for studying aging, a complex process that is regulated by multiple factors. Among these, mitochondria were shown to be of crucial importance. Recently, it was shown that the morphology of these organelles, which is dependent on dynamic fusion and fission processes, has profound effects on P. anserina aging. To further analyze this phenomenon, we characterized molecular components of the machinery regulating the dynamic behavior of mitochondria by utilizing transgenic strains in which fission genes (PaDnm1, PaFis1 and PaMdv1) and a fusion gene (PaFzo1) are overexpressed. While overexpression of PaFis1 has no phenotypic effects in the genetic background of the wild type, it surprisingly promotes mitochondrial fusion and decreases the life span in a mutant overexpressing PaDnm1. Remarkably, when grown on synthetic medium, overexpression of PaDnm1 leads to a decreased life span compared to the wild type. Increased expression of PaMdv1 results in the formation of ring-shaped mitochondria, a morphology of these organelles that has not been previously observed in P. anserina. Transformants with elevated PaFzo1 transcript levels show no altered life span, although the age-dependent fragmentation of mitochondria is impaired.
authors.affiliation: Johann W. Goethe-Universität, Institut für Molekulare Biowissenschaften, Frankfurt am Main, Germany. scheckhuber@bio.uni-frankfurt.de
authors.forename: Christian Q
authors.initials: CQ
authors.lastname: Scheckhuber
authors.forename: Elfie
authors.initials: E
authors.lastname: Rödel
authors.forename: Joycelyn
authors.initials: J
authors.lastname: Wüstehube
mesh_terms: D000375:Aging / Q000502:physiology*; D018797:Cell Cycle Proteins / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D008928:Mitochondria / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000502:physiology*; D008938:Mitosis / Q000502:physiology*; D048468:Podospora / Q000166:cytology* / Q000502:physiology*
chemical_list: D018797:Cell Cycle Proteins; D024101:Mitochondrial Proteins