Development of an X-ray HARP-FEA detector system for high-throughput protein crystallography.
A new detector system for protein crystallography is now being developed based on an X-ray HARP-FEA (high-gain avalanche rushing amorphous photoconductor-field emitter array), which consists of an amorphous selenium membrane and a matrix field emitter array. The combination of the membrane avalanche effect with a single driven FEA has several advantages over currently available area detectors, including higher sensitivity, higher spatial resolution and a higher frame rate. Preliminary evaluation of the detector has been carried out and its effectiveness has been confirmed. Next, diffraction images were measured with continuous rotation of a protein crystal, and the images were compared with those measured by the existing CCD detector; the system successfully obtained high-spatial-resolution images. Using shutterless measurement, the total measurement time can be reduced significantly, making the method appropriate for high-throughput protein crystallography. The X-ray HARP-FEA detector is an attractive candidate for the next generation of X-ray area detectors.
Miyoshi
Toshinobu
T
Institute of Materials Structure Science, High Energy Accelerator Research Organization, 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan. tmiyoshi@post.kek.jp
Igarashi
Noriyuki
N
Matsugaki
Naohiro
N
Yamada
Yusuke
Y
Hirano
Keiichi
K
Hyodo
Kazuyuki
K
Tanioka
Kenkichi
K
Egami
Norifumi
N
Namba
Masakazu
M
Kubota
Misao
M
Kawai
Teruo
T
Wakatsuki
Soichi
S
D018360:Crystallography, X-Ray / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity
D011506:Proteins