Methods to crystallize RNA.
Preparation of suitably large and well-ordered single crystals is usually the rate-limiting step in the determination of the three-dimensional structure of RNAs and their complexes with proteins by X-ray crystallography. This unit discusses a variety of experimental considerations for obtaining crystals of RNAs and RNA-protein complexes. Topics include design of crystallizable constructs, screening, and optimization of crystallization conditions.
Ferré-D'Amaré
A R
AR
Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Doudna
J A
JA
D000818:Animals; D001483:Base Sequence; D003460:Crystallization / Q000379:methods*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013768:Tetrahymena
D009843:Oligoribonucleotides; D011506:Proteins; D012313:RNA