title: Isolation of organelles and prefractionation of protein extracts using free-flow electrophoresis.
abstract: One of the major obstacles in the analysis of proteomes is the extreme complexity of any particular cell or biological fluid. Free-flow electrophoresis (FFE) is a powerful tool for reduction of this complexity, which is a prerequisite for systematic and comprehensive protein analyses. Protocols are provided in this unit for sample fractionation at two different stages: on the protein level by isoelectric focusing FFE fractionation of crude protein mixtures such as whole cell lysates, and on a subcellular level by zone-electrophoretic FFE purification of organelles.
authors.affiliation: Tecan Munich GmbH, Kirchheim, Germany.
authors.forename: Peter J A
authors.initials: PJA
authors.lastname: Weber
authors.affiliation: Tecan Munich GmbH, Kirchheim, Germany.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Weber
authors.affiliation: Tecan Munich GmbH, Kirchheim, Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Eckerskorn
mesh_terms: D004586:Electrophoresis / Q000295:instrumentation / Q000379:methods; D007525:Isoelectric Focusing / Q000379:methods; D015388:Organelles / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*
chemical_list: D011506:Proteins