title: Optic nerve degeneration in experimental autoimmune encephalomyelitis.
abstract: The mechanisms of axonal and neuronal degeneration causing disability in optic neuritis and multiple sclerosis are poorly understood. Here we describe the role of mitochondria, oxidative stress and the effects of modulating antioxidant gene expression in the optic nerves of mice induced with experimental autoimmune encephalomyelitis, with a focus on long-term neuroprotection. Oxidative injury to the mitochondrion began prior to inflammatory cell infiltration and continued. It affected subunits of the respiratory chain, glycolysis and a chaperone critical to the stabilization and import of proteins. Oxidative products were associated with loss of membrane potential, mitochondrial degeneration and severe axonal loss. Reductions in ATP synthesis were even greater than those associated with mitochondrial diseases. Increasing SOD2 levels by viral mediated gene transfer rescued ATP synthesis, suppressed myelin fiber injury and increased retinal ganglion cell survival 1 year later.
authors.affiliation: Department of Ophthalmology , University of Florida, College of Medicine, Gainesville, FL 32610-0284, USA. johnguy@eye.ufl.edu
authors.forename: John
authors.initials: J
authors.lastname: Guy
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001327:Autoimmune Diseases / Q000150:complications / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D004679:Encephalomyelitis / Q000150:complications* / Q000378:metabolism / Q000473:pathology; D008928:Mitochondria / Q000378:metabolism; D009901:Optic Nerve Diseases / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D013482:Superoxide Dismutase / Q000378:metabolism; D000099156:Superoxide Dismutase 2
chemical_list: D000255:Adenosine Triphosphate; D013482:Superoxide Dismutase; D000099156:Superoxide Dismutase 2