Electrically detected displacement assay (EDDA): a practical approach to nucleic acid testing in clinical or medical diagnosis.
This paper introduces the electrically detected displacement assay (EDDA), a electrical biosensor detection principle for applications in medical and clinical diagnosis, and compares the method to currently available microarray technologies in this field. The sensor can be integrated into automated systems of routine diagnosis, but may also be used as a sensor that is directly applied to the polymerase chain reaction (PCR) reaction vessel to detect unlabeled target amplicons within a few minutes. Major aspects of sensor assembly like immobilization procedure, accessibility of the capture probes, and prevention from nonspecific target adsorption, that are a prerequisite for a robust and reliable performance of the sensor, are demonstrated. Additionally, exemplary results from a human papillomavirus assay are presented.
Liepold
P
P
FRIZ Biochem Gesellschaft für Bioanalytik mbH, 82061, Neuried, Germany.
Kratzmüller
T
T
Persike
N
N
Bandilla
M
M
Hinz
M
M
Wieder
H
H
Hillebrandt
H
H
Ferrer
E
E
Hartwich
G
G
D001483:Base Sequence; D001681:Biological Assay / Q000379:methods*; D015374:Biosensing Techniques / Q000379:methods*; D004560:Electricity; D005838:Genotype; D006801:Humans; D046228:Microarray Analysis / Q000379:methods; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D008969:Molecular Sequence Data; D009696:Nucleic Acids / Q000032:analysis* / Q000235:genetics; D015345:Oligonucleotide Probes / Q000235:genetics; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D030361:Papillomavirus Infections / Q000175:diagnosis*; D016133:Polymerase Chain Reaction / Q000379:methods; D012680:Sensitivity and Specificity
D009696:Nucleic Acids; D015345:Oligonucleotide Probes