title: Adenoviruses C in non-hospitalized Mexican children older than five years of age with acute respiratory infection.
abstract: Adenoviruses (AdV) are commonly involved in acute respiratory infections (ARI), which cause high morbidity and mortality in children. AdV are grouped in six species (A-F), which are associated with a wide range of diseases. The aim of this study was to identify the AdV species infecting non-hospitalized Mexican children with ARI symptoms, attending to the same school. For that, a PCR/RFLP assay was designed for a region of the hexon gene, which was chosen, based on the bioinformatical analysis of AdV genomes obtained from GenBank. A total of 100 children's nasopharyngeal samples were collected from January to June, 2005, and used for viral isolation in A549 cells and PCR/RFLP analysis. Only 15 samples produced cytopathic effect, and in all of them AdV C was identified. AdV C was also identified in eight additional nasopharyngeal samples which were negative for viral isolation. In summary, this outpatient population showed a rate of AdV infection of 23%, and only AdV C was detected.
authors.affiliation: Laboratorio de Virología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Naciona, México, D.F., México.
authors.forename: Dora P
authors.initials: DP
authors.lastname: Rosete
authors.forename: María Eugenia
authors.initials: ME
authors.lastname: Manjarrez
authors.forename: Blanca L
authors.initials: BL
authors.lastname: Barrón
mesh_terms: D000208:Acute Disease; D000258:Adenovirus Infections, Human / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D000260:Adenoviruses, Human / Q000235:genetics* / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D002648:Child; D004262:DNA Restriction Enzymes / Q000032:analysis; D005260:Female; D005819:Genetic Markers; D016679:Genome, Viral; D006801:Humans; D008297:Male; D008800:Mexico / Q000453:epidemiology; D009305:Nasopharynx / Q000821:virology; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012141:Respiratory Tract Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D012621:Seasons
chemical_list: D005819:Genetic Markers; D004262:DNA Restriction Enzymes