Measurement of interleukin 7.
This unit describes an assay system to detect biologically active interleukin 7 (IL-7). The target or indicator cells are a clonally derived long-term pre-B cell line designated IxN/2bx (or, more simply, 2bx). These cells demonstrate an absolute specificity for IL-7 for continued growth and viability and are used to establish a thymidine-uptake proliferation assay similar to those established for other interleukins. A Support Protocol describes maintenance of the IxN/2bx cell line.
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Immunex Corporation, Seattle, Washington, USA.
Morrissey
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PJ
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D006801:Humans; D015851:Interleukin-7 / Q000032:analysis* / Q000502:physiology; D051379:Mice
D015851:Interleukin-7