title: [Establishment and preliminary application of multiplex PCR for detecting the microsatellite markers in silkies].
abstract: In order to obtain the stable PCR combinations for silkies genetic analysis, multiplex PCR was used and its reaction condition was optimized. Five combinations of multiplex PCR with good effects were obtained from eighteen microsatellite markers. Three of them were quadruplex-PCRs, and two were triplex-PCRs. The products of multiplex PCR were further combined into three sets for the electrophoresis on ABI 3100-Avant Genetic Analyzer. The results suggested that the eighteen microsatellite markers could be successfully applied to the silkies genetic analysis.
authors.affiliation: College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China. mxj0719@163.com
authors.forename: Xiang-Jun
authors.initials: XJ
authors.lastname: Meng
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhu
authors.forename: Ming-Ya
authors.initials: MY
authors.lastname: Zhang
authors.forename: Liang
authors.initials: L
authors.lastname: Li
authors.forename: Yi-Ping
authors.initials: YP
authors.lastname: Liu
mesh_terms: D000818:Animals; D002645:Chickens / Q000235:genetics*; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods*