The immunohistological observation of proliferation rule of Schwann cell after sciatic nerve injury in rats.
To describe the rule of the Schwann cell proliferation after peripheral nerve injury in detail and to discover the effect of neuroanastomosis, the model of rat sciatic injury was made, with neuroanastomosis on the left side and right side untreated. Then the materials were drawn the 24th hour, 48th hour, 4th day, 7th day, 14th day, 21st day after surgery. Immunohistological staining counted the schwann cells per view with Qw in software of Leica Ltd. The number of Schwann cells increased obviously on the 4th day after surgery and reached the peak in the 7th day. Then it fell down and the neuroanastomosis group changed slower and fibroblast hyperplasia in the untreated group. The axon support is essential for the Schwann cell. The precise rule is help for study on neurotrophic factor.
Zhang
Peixun
P
Department of Trauma and Orthopedics, People's Hospital, Peking University, Beijing, China.
Xue
Feng
F
Zhao
Fuqiang
F
Lu
Hao
H
Zhang
Hongbo
H
Jiang
Baoguo
B
D000818:Animals; D002450:Cell Communication; D049109:Cell Proliferation; D007150:Immunohistochemistry; D016130:Immunophenotyping; D008297:Male; D009409:Nerve Crush; D009416:Nerve Regeneration; D051381:Rats; D017207:Rats, Sprague-Dawley; D009418:S100 Proteins / Q000096:biosynthesis / Q000378:metabolism; D012583:Schwann Cells / Q000378:metabolism / Q000473:pathology*; D012584:Sciatic Nerve / Q000293:injuries* / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D020196:Trauma, Nervous System / Q000378:metabolism / Q000473:pathology; D014855:Wallerian Degeneration / Q000378:metabolism / Q000473:pathology
D009418:S100 Proteins