title: Immunocytochemical staining of p16(ink4a) protein as an adjunct test in equivocal liquid-based cytology.
abstract: For cervical cancer screening, HPV-DNA test is expensive and is not easily available in all clinical situations. Thus, we investigated the role of p16(ink4a) immunostaining as another adjunct test to diagnose cervical neoplasia in equivocal liquid based cytology. Eighty-seven patients were randomly selected for this study (3 patients with normal, 84 patients with abnormal including 24 ASCUS, 30 LSIL, and 30 HSIL). We performed p16(ink4a) immunostaining on ThinPrep slide and on each case from the corresponding cervical biopsy tissues. High-risk HPV-DNA testing was also performed on all the subjects. We found that the immunoreactivity of p16(ink4a) is strongly correlated with the grade of cytologic and histologic diagnoses as well as with Hybrid Capture 2. In comparing the p16(ink4a) immunostaining with the Hybrid Capture 2 for accuracy of the diagnosis of CIN II/III or a higher-grade disease in the case of ASCUS/LSIL on ThinPrep, no significant differences were observed. Our data implies that p16(ink4a) immunocytochemical staining in liquid-based cytology specimens might be used as a good adjunct test to predict cervical histology in equivocal ThinPrep tests.
authors.affiliation: Department of Obstetrics and Gynecology, College of Medicine, Ewha Womans University, Seoul, Korea.
authors.forename: Eun Kyoung
authors.initials: EK
authors.lastname: Shin
authors.forename: Sa Ra
authors.initials: SR
authors.lastname: Lee
authors.forename: Mi Kyoung
authors.initials: MK
authors.lastname: Kim
authors.forename: Eun Ji
authors.initials: EJ
authors.lastname: Kang
authors.forename: Woong
authors.initials: W
authors.lastname: Ju
authors.forename: Shi Nae
authors.initials: SN
authors.lastname: Lee
authors.forename: Woon Sup
authors.initials: WS
authors.lastname: Han
authors.forename: Seung Cheol
authors.initials: SC
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism* / Q000821:virology; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000378:metabolism*; D003581:Cytodiagnosis / Q000379:methods; D004273:DNA, Neoplasm / Q000032:analysis; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000175:diagnosis / Q000378:metabolism* / Q000821:virology; D015203:Reproducibility of Results; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000821:virology; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis / Q000378:metabolism* / Q000821:virology
chemical_list: D014408:Biomarkers, Tumor; D019941:Cyclin-Dependent Kinase Inhibitor p16; D004273:DNA, Neoplasm