Development of a universal nucleobase and modified nucleobases for expanding the genetic code.
This unit presents protocols for the synthesis and characterization of nucleosides with unnatural bases in order to develop bases for the expansion of the genetic alphabet or for nonselective pairing opposite natural bases. Protocols describe the design, synthesis, and characterization of unnatural base pairs involving 1-beta-D-2-deoxyribosyl-N- and -C-nucleosides. Determination of the thermodynamic and kinetic parameters of unnatural nucleosides is accomplished by incorporation into oligonucleotides and subsequent evaluation as described herein.
Romesberg
Floyd E
FE
The Scripps Research Institute, La Jolla, California, USA.
Yu
Chengzhi
C
Matsuda
Shigeo
S
Henry
Allison A
AA
D020029:Base Pairing; D005815:Genetic Code; D007700:Kinetics; D008722:Methods; D009705:Nucleosides / Q000138:chemical synthesis* / Q000737:chemistry; D013816:Thermodynamics
D009705:Nucleosides