title: Interaction trap/two-hybrid system to identify interacting proteins.
abstract: The parameters and experimental conditions for this important system are constantly undergoing improvement. This newest version includes expanded tables describing interaction trap components and additional libraries compatible with the interaction trap system. It also features a new protocol on performing a hunt by interaction mating. Some of the commercial vendors selling yeast two-hybrid reagents recommend using interaction mating to perform a hunt, so this procedure should be of great interest.
authors.affiliation: Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA. EA_Golemis@fccc.edu
authors.forename: E A
authors.initials: EA
authors.lastname: Golemis
authors.forename: I
authors.initials: I
authors.lastname: Serebriiskii
authors.forename: R L
authors.initials: RL
authors.lastname: Finley
authors.forename: M G
authors.initials: MG
authors.lastname: Kolonin
authors.forename: J
authors.initials: J
authors.lastname: Gyuris
authors.forename: R
authors.initials: R
authors.lastname: Brent
mesh_terms: D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D008954:Models, Biological; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D002352:Carrier Proteins; D011506:Proteins