Decylubiquinol impedes mitochondrial respiratory chain complex I activity.
We have studied the interaction of decylubiquinone, an effective substrate for respiratory chain complexes III and II, with complex I in mouse and human tissues. We found that its reduced form, decylubiquinol, severely impedes complex I activity, while the oxidized form, decylubiquinone acts as a potent acceptor for complex I electrons. This observation has obvious incidence on the assay conditions for complex I. In keeping with that, we found that the inhibition by the reduced form can be avoided by maintaining decylubiquinone under an oxidized form. Under these experimental conditions, a high complex I activity could be measured allowing to detect partial complex I deficiency. Use of these conditions is however restricted to tissues/cells with limited contaminating NADH dehydrogenase activities that are prone to react with redox active compounds.
Bénit
Paule
P
Inserm, U676, Hôpital Robert Debré, Bât Ecran, Paris, France.
Slama
Abdelhamid
A
Rustin
Pierre
P
D015086:2,6-Dichloroindophenol / Q000494:pharmacology; D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D042967:Electron Transport Complex I / Q000037:antagonists & inhibitors* / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D009206:Myocardium / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D010084:Oxidation-Reduction; D012402:Rotenone / Q000494:pharmacology; D013481:Superoxides / Q000378:metabolism; D014018:Tissue Distribution; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D014475:Uncoupling Agents / Q000494:pharmacology
D002457:Cell Extracts; D009097:Multienzyme Complexes; D014475:Uncoupling Agents; D012402:Rotenone; D013481:Superoxides; D014451:Ubiquinone; C060262:2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone; D015086:2,6-Dichloroindophenol; C023107:NADH oxidase; D009247:NADH, NADPH Oxidoreductases; D042967:Electron Transport Complex I