Pulse sequence for dynamic volumetric imaging of hyperpolarized metabolic products.
Dynamic nuclear polarization and dissolution of a (13)C-labeled substrate enables the dynamic imaging of cellular metabolism. Spectroscopic information is typically acquired, making the acquisition of dynamic volumetric data a challenge. To enable rapid volumetric imaging, a spectral-spatial excitation pulse was designed to excite a single line of the carbon spectrum. With only a single resonance present in the signal, an echo-planar readout trajectory could be used to resolve spatial information, giving full volume coverage of 32 x 32 x 16 voxels every 3.5s. This high frame rate was used to measure the different lactate dynamics in different tissues in a normal rat model and a mouse model of prostate cancer.
Cunningham
Charles H
CH
Department of Medical Biophysics and Sunnybrook Health Sciences Centre, 2075 Bayview Avenue, Toronto, Ont., Canada M4N 3M5. chuck@sri.utoronto.ca
Chen
Albert P
AP
Lustig
Michael
M
Hargreaves
Brian A
BA
Lupo
Janine
J
Xu
Duan
D
Kurhanewicz
John
J
Hurd
Ralph E
RE
Pauly
John M
JM
Nelson
Sarah J
SJ
Vigneron
Daniel B
DB
D000230:Adenocarcinoma / Q000378:metabolism*; D000818:Animals; D002247:Carbon Isotopes; D007773:Lactates / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D051379:Mice; D019047:Phantoms, Imaging; D011471:Prostatic Neoplasms / Q000378:metabolism*; D011773:Pyruvates / Q000378:metabolism; D051381:Rats
D002247:Carbon Isotopes; D007773:Lactates; D011773:Pyruvates