title: Cosmetic effectiveness of topically applied hydrolysed keratin peptides and lipids derived from wool.
abstract: OBJECTIVE
  Skin moisturisation, elasticity, feel and appearance can all be improved through the topical application of protein hydrolysates. Recent studies suggest that supplementing intercellular lipids of the stratum corneum can enhance the functioning of the skin.
  
  
  METHODS
  In this study, a hydrolysed keratin peptide (molecular weight <1000 Da) was prepared from wool and tested on skin in two different formulations: an aqueous solution and an internal wool lipids (IWL) liposome suspension. In vivo long-term studies were performed to evaluate the water barrier function of the skin after topical application of different formulations. During the treatment period, hydration and elasticity were determined. A sorption-desorption test was also performed to assess the hygroscopic properties and water-holding capacity of the different treated skin sites.
  
  
  RESULTS
  Significant differences were found between the control and treated sites, with the treated areas showing an increase in hydration and elasticity as a result of keratin peptide application. Measurements also indicated that the keratin formulations reinforce the skin barrier integrity, improving its water-holding capacity.
  
  
  CONCLUSIONS
  A combination of the keratin peptide with the IWL showed beneficial effects, indicating that this combination is suitable for designing new cosmetics products.
authors.affiliation: Department of Surfactant Technology, Chemical and Environmental Research Institute of Barcelona, Barcelona, Spain. cbaesl@iiqab.csic.es
authors.forename: C
authors.initials: C
authors.lastname: Barba
authors.forename: S
authors.initials: S
authors.lastname: Méndez
authors.forename: A
authors.initials: A
authors.lastname: Roddick-Lanzilotta
authors.forename: R
authors.initials: R
authors.lastname: Kelly
authors.forename: J L
authors.initials: JL
authors.lastname: Parra
authors.forename: L
authors.initials: L
authors.lastname: Coderch
mesh_terms: D000287:Administration, Topical; D000328:Adult; D000818:Animals; D001834:Body Water / Q000378:metabolism*; D003358:Cosmetics / Q000008:administration & dosage*; D003879:Dermatologic Agents / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D004548:Elasticity; D005260:Female; D006244:Hardness; D006801:Humans; D006868:Hydrolysis; D007633:Keratins / Q000008:administration & dosage*; D008055:Lipids / Q000008:administration & dosage*; D010455:Peptides / Q000008:administration & dosage*; D012756:Sheep; D012869:Skin Absorption / Q000187:drug effects / Q000502:physiology*; D016896:Treatment Outcome; D014935:Wool / Q000737:chemistry*
chemical_list: D003358:Cosmetics; D003879:Dermatologic Agents; D008055:Lipids; D010455:Peptides; D007633:Keratins