[Determination of vitamin D in calcium fortified foods using solid-phase extraction-high performuance liquid chromatography].
A method for the determination of vitamin D in calcium fortified foods using solid-phase extraction-high performance liquid chromatography has been developed. The Chromabond XTR solid-phase extraction column (14,500 mg, 70 mL) was used to extract and clean-up the sample. The calibration curve of vitamin D showed good linearity in the range of 0.1-100.0 microg/mL with correlation coefficient of 0.999. The limit of qualification was 0.01 microg/g and the limit of quantification was 0.03 microg/g. The average recoveries at three spiking levels were 106.2%, 99.5%, 100.1%, and the relative standard deviations were lower than 10%.
Zhao
Rong
R
Beijing Center for Disease Prevention anld Conttroli, Beijing 100013, China.
Xue
Ying
Y
Wu
Guohua
G
Zhao
Haiyan
H
Luo
Rencai
R
D053000:Analytic Sample Preparation Methods; D002118:Calcium; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000592:standards; D005504:Food Analysis / Q000379:methods* / Q000592:standards; D005527:Food, Fortified / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D012015:Reference Standards; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D013997:Time Factors; D014807:Vitamin D / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification
D012997:Solvents; D014807:Vitamin D; D002118:Calcium