Oxidative alterations induced by D-aspartic acid in prepubertal rat testis in vitro: a mechanistic study.
The objective of the present study was to investigate the oxidative induction response following in vitro treatment with D-aspartic acid (DA) in prepubertal rat testis (homogenates, explants, and cell suspensions). In all three preparations, DA enhanced (P<0.001) lipid peroxidation, manifest as increased reactive oxygen species (ROS) and malondialdehyde (MDA). Further, DA-induced oxidative induction was potentiated (P<0.001) in the presence of iron (5 microM) and 3-amino triazole and mercaptosuccinate (P<0.001), known inhibitors of the peroxide metabolizing enzymes, catalase and glutathione peroxidase, respectively. Testis homogenates exposed to L-arginine (LA) per se had reduced (P<0.001) endogenous levels of ROS and MDA; furthermore, pre-incubation with L-arginine markedly suppressed (P<0.001) DA-induced oxidative induction, suggesting an antagonistic action, perhaps due to LA-derived nitric oxide. In conclusion, DA caused significant oxidative induction in prepubertal rat testis, but this action was abrogated by L-arginine. The relevance of this phenomenon in vivo merits further study, as both of these molecules have specific physiological functions in the testis.
Chandrashekar
K N
KN
Department of Biochemistry & Nutrition, Central Food Technological Research Institute, VV Mohalla, Mysore, Karnataka, India.
Muralidhara
D000640:Amitrole / Q000494:pharmacology; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002478:Cells, Cultured; D026603:D-Aspartic Acid / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007501:Iron / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013870:Thiomalates / Q000494:pharmacology; D014131:Trace Elements / Q000494:pharmacology
D004791:Enzyme Inhibitors; D017382:Reactive Oxygen Species; D013870:Thiomalates; D014131:Trace Elements; D009569:Nitric Oxide; D026603:D-Aspartic Acid; C046062:2-thiomalic acid; D001120:Arginine; D006861:Hydrogen Peroxide; D007501:Iron; D000640:Amitrole