Direct comparison between ICSI-mediated DNA transfer and pronuclear DNA microinjection for producing transgenic rats.
Production efficiency of transgenic rats was compared directly between the routine pronuclear microinjection of exogenous DNA solution (PNMI-Tg method) and the ooplasmic injection of sperm cells exposed to exogenous DNA solution (ICSI-Tg method) using six DNA constructs. The overall production efficiency per treated oocyte in the ICSI-Tg method (mean 1.1%, range 0.2 to 3.1%) was similar to that in the PNMI-Tg method (mean 1.1%, range 0 to 2.4%). An advantage of the ICSI-Tg method in the production of transgenic rats is noted in cases in which a low yield of pronuclear zygotes is an inevitable fate of the rat strain.
Hirabayashi
Masumi
M
National Institute for Physiological Sciences, Okazaki, Aichi, Japan.
Kato
Megumi
M
Amemiya
Kazue
K
Hochi
Shinichi
S
D000818:Animals; D030801:Animals, Genetically Modified; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics*; D005260:Female; D018014:Gene Transfer Techniques; D008845:Microinjections / Q000379:methods*; D009865:Oocytes; D051381:Rats / Q000235:genetics*; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods*
D004247:DNA