title: Imaging GABAc receptors with ligand-conjugated quantum dots.
abstract: We report a methodology for labeling the GABAc receptor on the surface membrane of intact cells. This work builds upon our earlier work with serotonin-conjugated quantum dots and our studies with PEGylated quantum dots to reduce nonspecific binding. In the current approach, a PEGylated derivative of muscimol was synthesized and attached via an amide linkage to quantum dots coated in an amphiphilic polymer derivative of a modified polyacrylamide. These conjugates were used to image GABA(C) receptors heterologously expressed in Xenopus laevis oocytes.
authors.affiliation: Department of Chemistry, Vanderbilt University, Nashville, TN 37235, USA.
authors.forename: Ian D
authors.initials: ID
authors.lastname: Tomlinson
authors.forename: Hélène A
authors.initials: HA
authors.lastname: Gussin
authors.forename: Deborah M
authors.initials: DM
authors.lastname: Little
authors.forename: Michael R
authors.initials: MR
authors.lastname: Warnement
authors.forename: Haohua
authors.initials: H
authors.lastname: Qian
authors.forename: David R
authors.initials: DR
authors.lastname: Pepperberg
authors.forename: Sandra J
authors.initials: SJ
authors.lastname: Rosenthal