title: [Study of the subcellular location of the human gene 6 transactivated by nonstructeral protein 5a of hepatitis C virus].
abstract: OBJECTIVE
  To found the subcellular location of the human gene 6 transactivated by nonstructural protein 5A of hepatitis C virus (NS5ATP6).
  
  
  METHODS
  Green fluorescent protein (GFP) expression vector pEGFP- NS5ATP6 was established. The pEGFP- NS5ATP6 was transfected into HepG2 cells, and analyze the subcellular location of the proteins expressed by NS5ATP6 through Green fluorescent microscopy after 24 hours.
  
  
  RESULTS
  The pEGFP- NS5ATP6 gene was successful cloned, NS5ATP6 can express protein in cells and subcellularly located in cell plasma.
  
  
  CONCLUSIONS
  NS5ATP6 can express protein, and the protein expressed by NS5ATP6 subcellularly located in cell plasma.
authors.affiliation: The 302 Hospital of PLA, Beijing 100039 China.
authors.forename: Jian-jun
authors.initials: JJ
authors.lastname: Wang
authors.forename: Ping
authors.initials: P
authors.lastname: Zhao
authors.forename: Xue-yuan
authors.initials: XY
authors.lastname: Jin
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.forename: Song
authors.initials: S
authors.lastname: Qing
authors.forename: Ning
authors.initials: N
authors.lastname: Ding
mesh_terms: D045744:Cell Line, Tumor; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D016174:Hepacivirus; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D015533:Transcriptional Activation; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase
chemical_list: D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase