title: Antibiofilm activity of sodium bicarbonate, sodium metaperiodate and SDS combination against dental unit waterline-associated bacteria and yeast.
abstract: OBJECTIVE
  To determine the effect of sodium bicarbonate (SB), sodium metaperiodate (SMP) and sodium dodecyl sulfate (SDS) combination on biofilm formation and dispersal in dental unit waterline (DUWL)-associated bacteria and yeast.
  
  
  RESULTS
  The in vitro effect of SB, SMP and SDS alone and in combination on biofilm formation and dispersal in Pseudomonas aeruginosa, Klebsiella pneumoniae, Actinomyces naeslundii, and Candida albicans was investigated using a 96-well microtitre plate biofilm assay. The combination showed a broad-spectrum inhibitory effect on growth as well as biofilm formation of both gram-negative and gram-positive bacteria, and yeast. In addition, the SB + SMP + SDS combination was significantly more effective in dispersing biofilm than the individual compounds. The combination dispersed more than 90% of P. aeruginosa biofilm whereas the commercial products, Oxygenal 6, Sterilex Ultra, and PeraSafe showed no biofilm dispersal activity.
  
  
  CONCLUSIONS
  The composition comprising SB, SMP, and SDS was effective in inhibiting as well as dispersing biofilms in DUWL-associated bacteria and yeast.
  
  
  CONCLUSIONS
  This study shows that a composition comprising environmentally friendly and biologically safe compounds such as SB, SMP, and SDS has a potential application in reducing DUWL-associated acquired infections in dental clinics.
authors.affiliation: Kane Biotech Inc., Winnipeg, MB, Canada.
authors.forename: P V
authors.initials: PV
authors.lastname: Gawande
authors.forename: K
authors.initials: K
authors.lastname: LoVetri
authors.forename: N
authors.initials: N
authors.lastname: Yakandawala
authors.forename: T
authors.initials: T
authors.lastname: Romeo
authors.forename: G G
authors.initials: GG
authors.lastname: Zhanel
authors.forename: D G
authors.initials: DG
authors.lastname: Cvitkovitch
authors.forename: S
authors.initials: S
authors.lastname: Madhyastha
mesh_terms: D001419:Bacteria / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D019551:Dental Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D006801:Humans; D019552:Infection Control, Dental / Q000379:methods*; D008826:Microbial Sensitivity Tests; D008934:Mitogens / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D017693:Sodium Bicarbonate / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D014871:Water Microbiology; D015003:Yeasts / Q000187:drug effects
chemical_list: D019551:Dental Disinfectants; D008934:Mitogens; D013501:Surface-Active Agents; D010504:Periodic Acid; D012967:Sodium Dodecyl Sulfate; D017693:Sodium Bicarbonate; C009288:metaperiodate