Culture and PCR analysis of joint fluid in the diagnosis of prosthetic joint infection.
This prospective study compared PCR and culture techniques in the diagnosis of prosthetic joint infection (PJI). We obtained joint fluid samples (JFS; n=115) from patients who had failed total joint arthroplasty between January 2003 and June 2005; 49 were positive for PJI according to established strict criteria. JFS were analyzed by PCR (n=35; control n=66) or culture (n=46, control n=48). PCR was positive in 71% of PJI cases, resulting in sensitivity, specificity, accuracy, positive predictive value, negative predictive value, and likelihood ratio for positive results as follows: 0.71; 0.97; 0.88; 0.93; 0.87 and 23.6, respectively. Culture was positive in 44% of PJI samples. Corresponding statistics were 0.44; 0.94; 0.69; 0.87; 0.63 and 7.0, respectively. Significantly higher sensitivity, accuracy and negative predictive values were calculated for PCR versus culture, and there was 83% concordance between the results of intraoperative culture and PCR detection of causative bacteria. Therefore, we conclude that PCR analysis of synovial fluid increases the utility of pre-operative aspiration for patients who require revision total joint surgery.
Gallo
Jiri
J
Department of Orthopaedics, University Hospital and Faculty of Medicine and Dentistry, Palacký University, I.P. Pavlova 6, Olomouc, Czech Republic. jiri.gallo@volny.cz
Kolar
Milan
M
Dendis
Milos
M
Loveckova
Yvona
Y
Sauer
Pavel
P
Zapletalova
Jana
J
Koukalova
Dagmar
D
D019643:Arthroplasty, Replacement / Q000009:adverse effects*; D001424:Bacterial Infections / Q000175:diagnosis / Q000382:microbiology; D001431:Bacteriological Techniques; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification; D006094:Gram-Positive Bacteria / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D011237:Predictive Value of Tests; D016459:Prosthesis-Related Infections / Q000175:diagnosis* / Q000382:microbiology*; D012680:Sensitivity and Specificity; D013582:Synovial Fluid / Q000382:microbiology*
D003470:Culture Media; D004269:DNA, Bacterial