title: An investigation of vibration-induced protein desorption mechanism using a micromachined membrane and PZT plate.
abstract: A micromachined vibrating membrane is used to remove adsorbed proteins on a surface. A lead zirconate titanate (PZT) composite (3 x 1 x 0.5 mm) is attached to a silicon membrane (2,000 x 500 x 3 microm) and vibrates in a flexural plate wave (FPW) mode with wavelength of 4,000/3 microm at a resonant frequency of 308 kHz. The surface charge on the membrane and fluid shear stress contribute in minimizing the protein adsorption on the SiO(2) surface. In vitro characterization shows that 57 +/- 10% of the adsorbed bovine serum albumin (BSA), 47 +/- 13% of the immunoglobulin G (IgG), and 55.3~59.2 +/- 8% of the proteins from blood plasma are effectively removed from the vibrating surface. A simulation study of the vibration-frequency spectrum and vibrating amplitude distribution matches well with the experimental data. Potentially, a microelectromechanical system (MEMS)-based vibrating membrane could be the tool to minimize biofouling of in vivo MEMS devices.
authors.affiliation: Department of Mechanical Engineering, University of British Columbia, 2054-6250 Applied Science Lane, Vancouver, British Columbia, V6T 1Z4, Canada.
authors.forename: Po Ying
authors.initials: PY
authors.lastname: Yeh
authors.forename: Yevgeniya
authors.initials: Y
authors.lastname: Le
authors.forename: Jayachandran N
authors.initials: JN
authors.lastname: Kizhakkedathu
authors.forename: Mu
authors.initials: M
authors.lastname: Chiao
mesh_terms: D000327:Adsorption; D001798:Blood Proteins / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000648:ultrastructure; D003198:Computer Simulation; D004867:Equipment Design; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D019084:Fluorescent Antibody Technique, Indirect; D005456:Fluorescent Dyes / Q000378:metabolism; D007074:Immunoglobulin G / Q000302:isolation & purification / Q000648:ultrastructure; D007700:Kinetics; D007854:Lead / Q000737:chemistry*; D008567:Membranes, Artificial; D011506:Proteins / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000648:ultrastructure; D012710:Serum Albumin, Bovine / Q000302:isolation & purification / Q000493:pharmacokinetics / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*; D014732:Vibration; D015040:Zirconium / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D005456:Fluorescent Dyes; D007074:Immunoglobulin G; D008567:Membranes, Artificial; D011506:Proteins; D012710:Serum Albumin, Bovine; D007854:Lead; D012822:Silicon Dioxide; D015040:Zirconium; D014025:Titanium; D016650:Fluorescein-5-isothiocyanate