title: Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice.
abstract: This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.
authors.affiliation: Department of Pediatric Dentistry, School of Dentistry of Araraquara, State University of São Paulo, Araraquara, SP, Brazil. tetelaguna@gmail.com
authors.forename: Ana Teresa
authors.initials: AT
authors.lastname: Sant'anna
authors.forename: Luis Carlos
authors.initials: LC
authors.lastname: Spolidório
authors.forename: Lizeti Toledo Oliveira
authors.initials: LT
authors.lastname: Ramalho
mesh_terms: D000818:Animals; D003238:Connective Tissue / Q000187:drug effects / Q000473:pathology; D004731:Endotoxins / Q000009:adverse effects*; D004926:Escherichia coli; D005355:Fibrosis; D005566:Formocresols / Q000494:pharmacology*; D015743:Giant Cells, Foreign-Body / Q000187:drug effects / Q000473:pathology; D006097:Granulation Tissue / Q000187:drug effects / Q000473:pathology; D007249:Inflammation; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology; D008070:Lipopolysaccharides / Q000009:adverse effects; D008214:Lymphocytes / Q000187:drug effects / Q000473:pathology; D008264:Macrophages / Q000187:drug effects / Q000473:pathology; D051379:Mice; D009336:Necrosis; D009504:Neutrophils / Q000187:drug effects / Q000473:pathology; D010950:Plasma Cells / Q000187:drug effects / Q000473:pathology; D011897:Random Allocation; D012388:Root Canal Irrigants / Q000494:pharmacology*; D040521:Subcutaneous Tissue / Q000187:drug effects* / Q000473:pathology; D013997:Time Factors; D014664:Vasodilation / Q000187:drug effects
chemical_list: D004731:Endotoxins; D005566:Formocresols; D008070:Lipopolysaccharides; D012388:Root Canal Irrigants; C031025:formocresol