Mutagenesis approaches for elucidation of protein structure-function relationships.
Several mutagenesis strategies have been used to allow the identification of specific receptor-ligand interactions to support the docking of ligands within various receptor models. The strengths and limitations of each strategy are discussed in this overview. Large-scale mapping is described using deletion and chimeric receptors. Fine tuning with single residue replacements is also covered along with two-dimensional approaches.
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Schering-Plough Research Institute, Kenilworth, New Jersey, USA.
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D006801:Humans; D008024:Ligands; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D016296:Mutagenesis / Q000235:genetics*; D013329:Structure-Activity Relationship
D008024:Ligands; D008565:Membrane Proteins