HPLC of peptides and proteins.
High-performance liquid chromatography (HPLC) is an essential tool for the purification and characterization of biomacromolecules. This unit presents a thorough discussion of the eight types of HPLC currently used, highlighting equipment and start-up procedures, recommendations for running each type of experiment, and theoretical considerations for the separation of peptides and proteins. This is an excellent primer for HPLC users.
Boysen
Reinhard I
RI
Monash University, Victoria, Australia.
Hearn
Milton T W
MTW
Monash University, Victoria, Australia.
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D015203:Reproducibility of Results
D010455:Peptides; D011506:Proteins