Antiproliferative activity of aucubin is through cell cycle arrest and apoptosis in human non-small cell lung cancer A549 cells.
Aucubin, an iridoid glycoside isolated from the leaves of Aucuba japonica, inhibits human non-small cell lung cancer A549 cells by blocking cell cycle progression in the G(0)/G(1) phase and inducing apoptosis. An ELISA showed that the G(0)/G(1) phase arrest is due to p53-mediated induction of p21. Enhancement of Fas and its two ligands, membrane-bound and soluble Fas ligand, may be responsible for the apoptotic effect induced by aucubin. The present study shows, for the first time, that the induction of p53 and activity of the Fas/Fas ligand apoptotic system may participate in the antiproliferative activity of aucubin in A549 cells.
Hung
Jen-Yu
JY
Division of Pulmonary and Critical Care Medicine, Kaohsiung Medical University Hospita, Kaohsiung, Taiwan, China.
Yang
Chih-Jen
CJ
Tsai
Ying-Ming
YM
Huang
Hurng-Wern
HW
Huang
Ming-Shyan
MS
D000906:Antibodies / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics; D004353:Drug Evaluation, Preclinical; D053222:Fas Ligand Protein / Q000235:genetics / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D016158:Genes, p53; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D057888:Iridoid Glucosides; D039823:Iridoids / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D019014:fas Receptor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000276:immunology / Q000502:physiology
D000906:Antibodies; D000970:Antineoplastic Agents; C493956:CDKN1A protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; C505661:FASLG protein, human; D053222:Fas Ligand Protein; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D019014:fas Receptor; C006650:aucubin