title: The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi.
abstract: The exonuclease ERI-1 negatively regulates RNA interference in Caenorhabditis elegans and Schizosaccharomyces pombe, and is required for production of some C. elegans endogenous small interfering RNAs. We show that ERI-1 performs 3' end processing of the 5.8S ribosomal RNA in both C. elegans and S. pombe. In C. elegans, two protein isoforms of ERI-1 are localized to the cytoplasm, and each has distinct functions in ribosomal RNA processing and negative regulation of RNA interference.
authors.affiliation: Department of Genetics, Harvard Medical School, and Department of Molecular Biology, Massachusetts General Hospital, CPZN7250, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
authors.forename: Harrison W
authors.initials: HW
authors.lastname: Gabel
authors.forename: Gary
authors.initials: G
authors.lastname: Ruvkun
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000378:metabolism; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D019143:Evolution, Molecular; D005095:Exoribonucleases / Q000378:metabolism*; D034622:RNA Interference; D012323:RNA Processing, Post-Transcriptional; D012568:Schizosaccharomyces / Q000201:enzymology* / Q000378:metabolism
chemical_list: D029742:Caenorhabditis elegans Proteins; C482202:ERI-1 protein, C elegans; C511955:Eri1 protein, S pombe; D005095:Exoribonucleases