A fluorescent intercalator displacement assay for establishing DNA binding selectivity and affinity.
A protocol for a fluorescent intercalator displacement (FID) assay useful for establishing DNA binding selectivity, affinity, stoichiometry, and binding site size, and for distinguishing modes of DNA binding is presented.
Tse
Winston C
WC
Gilead Sciences, Foster City, California, USA.
Boger
Dale L
DL
D001665:Binding Sites; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004996:Ethidium / Q000737:chemistry / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000378:metabolism; D007364:Intercalating Agents / Q000737:chemistry* / Q000378:metabolism; D009838:Oligodeoxyribonucleotides / Q000737:chemistry / Q000378:metabolism; D013050:Spectrometry, Fluorescence / Q000379:methods*
D005456:Fluorescent Dyes; D007364:Intercalating Agents; D009838:Oligodeoxyribonucleotides; D004247:DNA; D004996:Ethidium