title: Isotopic labelling of photosystem II in Thermosynechococcus elongatus.
abstract: This report describes a protocol to incorporate isotopically labelled aromatic amino acids into the proteins of the thermophilic cyanobacterium Thermosynechoccus elongatus. By using the EPR signal of the two redox active tyrosines of Photosystem II, Tyr(D)(*) and Tyr(Z)(*), as spectroscopic probes it is shown that labelled tyrosines can be incorporated with a high yield in this cyanobacterium. The production of a fully (13)C- or (2)H-labelled enzyme is also described.
authors.affiliation: iBiTec-S, URA CNRS 2096, CEA Saclay, 91191, Gif sur Yvette, France. alain.boussac@cea.fr
authors.forename: Alain
authors.initials: A
authors.lastname: Boussac
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Verbavatz
authors.forename: Miwa
authors.initials: M
authors.lastname: Sugiura
mesh_terms: D000458:Cyanobacteria / Q000378:metabolism*; D004578:Electron Spin Resonance Spectroscopy; D007553:Isotope Labeling; D045332:Photosystem II Protein Complex / Q000378:metabolism*; D014443:Tyrosine / Q000031:analogs & derivatives* / Q000378:metabolism
chemical_list: D045332:Photosystem II Protein Complex; C066447:tyrosyl radical Y(D); C114979:tyrosyl radical YZ; D014443:Tyrosine