title: Development of an X-ray HARP-FEA detector system for high-throughput protein crystallography.
abstract: A new detector system for protein crystallography is now being developed based on an X-ray HARP-FEA (high-gain avalanche rushing amorphous photoconductor-field emitter array), which consists of an amorphous selenium membrane and a matrix field emitter array. The combination of the membrane avalanche effect with a single driven FEA has several advantages over currently available area detectors, including higher sensitivity, higher spatial resolution and a higher frame rate. Preliminary evaluation of the detector has been carried out and its effectiveness has been confirmed. Next, diffraction images were measured with continuous rotation of a protein crystal, and the images were compared with those measured by the existing CCD detector; the system successfully obtained high-spatial-resolution images. Using shutterless measurement, the total measurement time can be reduced significantly, making the method appropriate for high-throughput protein crystallography. The X-ray HARP-FEA detector is an attractive candidate for the next generation of X-ray area detectors.
authors.affiliation: Institute of Materials Structure Science, High Energy Accelerator Research Organization, 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan. tmiyoshi@post.kek.jp
authors.forename: Toshinobu
authors.initials: T
authors.lastname: Miyoshi
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Igarashi
authors.forename: Naohiro
authors.initials: N
authors.lastname: Matsugaki
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Yamada
authors.forename: Keiichi
authors.initials: K
authors.lastname: Hirano
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hyodo
authors.forename: Kenkichi
authors.initials: K
authors.lastname: Tanioka
authors.forename: Norifumi
authors.initials: N
authors.lastname: Egami
authors.forename: Masakazu
authors.initials: M
authors.lastname: Namba
authors.forename: Misao
authors.initials: M
authors.lastname: Kubota
authors.forename: Teruo
authors.initials: T
authors.lastname: Kawai
authors.forename: Soichi
authors.initials: S
authors.lastname: Wakatsuki
mesh_terms: D018360:Crystallography, X-Ray / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D011506:Proteins