title: Interchromatidal central ridge and transversal symmetry in early metaphasic human chromosome one.
abstract: The topographic structure of Giemsa-banded (G-banded) early metaphase human chromosomes adsorbed on glass was analyzed by atomic force microscope using amplitude modulation mode (AM-AFM). Longitudinal height measurements for early metaphasic human chromosomes showed a central ridge that was further characterized by transversal height measurements. The heterochromatic regions displayed a high level of transversal symmetry, while the euchromatic ones presented several peaks across the transversal height measurements. We suggest that this central ridge and symmetry patterns point out a transitional arrangement of the early metaphase chromosome and support evidence for interchromatidal interactions prior to disjunction.
authors.affiliation: Escuela de Ciencias Biológicas, Universidad Nacional de Costa Rica, Costa Rica. oarguello@cenat.ac.cr
authors.forename: Orlando
authors.initials: O
authors.lastname: Argüello-Miranda
authors.forename: Giovanni
authors.initials: G
authors.lastname: Sáenz-Arce
mesh_terms: D000328:Adult; D002842:Chromatids / Q000378:metabolism* / Q000648:ultrastructure; D002871:Chromosome Banding; D002878:Chromosomes, Human, Pair 1 / Q000378:metabolism* / Q000648:ultrastructure; D022041:Euchromatin / Q000648:ultrastructure; D005260:Female; D006570:Heterochromatin / Q000648:ultrastructure; D006801:Humans; D008297:Male; D008677:Metaphase
chemical_list: D022041:Euchromatin; D006570:Heterochromatin