Effect of budesonide and formoterol on IL-6 and IL-8 release from primary bronchial epithelial cells.
The aim of this study was to explore possible interactions between a glucocorticosteroid (budesonide) and a long-acting beta(2)-agonist (formoterol) on pro-inflammatory cytokine release. Primary bronchial epithelial cells (PBEC) were stimulated with organic dust and incubated with budesonide, formoterol, and the combination of both drugs. Interleukin (IL)-6 and -8 in the supernatant was assessed by enzyme-linked immunosorbent assay (ELISA). Formoterol increased the IL-6 release but did not influence the IL-8 release. Budesonide attenuated the IL-6 and IL-8 release, an inhibiting effect that was sustained, but not reinforced, by formoterol.
Strandberg
Karin
K
Lung and Allergy Research, The National Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden. karin.strandberg@ki.se
Palmberg
Lena
L
Larsson
Kjell
K
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D001980:Bronchi / Q000166:cytology / Q000276:immunology*; D019819:Budesonide / Q000494:pharmacology*; D002478:Cells, Cultured; D004391:Dust / Q000276:immunology; D004847:Epithelial Cells / Q000187:drug effects / Q000276:immunology; D004983:Ethanolamines / Q000494:pharmacology*; D000068759:Formoterol Fumarate; D005938:Glucocorticoids / Q000494:pharmacology*; D006801:Humans; D015850:Interleukin-6 / Q000276:immunology*; D016209:Interleukin-8 / Q000276:immunology*
D058666:Adrenergic beta-2 Receptor Agonists; D000318:Adrenergic beta-Agonists; D004391:Dust; D004983:Ethanolamines; D005938:Glucocorticoids; D015850:Interleukin-6; D016209:Interleukin-8; D019819:Budesonide; D000068759:Formoterol Fumarate