title: A comparison of anionic nanoparticles and microparticles as vaccine delivery systems.
abstract: The objective of this work was to conduct an in vivo comparison of nanoparticles and microparticles as vaccine delivery systems. Poly (lactide-co-glycolide) (PLG) polymers were used to create nanoparticles size 110 nm and microparticles of size 800-900 nm. Protein antigens were then adsorbed to these particles. The efficacy of these delivery systems was tested with two protein antigens. A recombinant antigen from Neisseria meningitides type B (MenB) was administered intramuscularly (i.m.) or intraperitonealy (i.p.). An antigen from HIV-1, env glycoprotein gp140 was administered intranasally (i.n.) followed by an i.m. boost. From three studies, there were no differences between the nanoparticles and micro-particles formulations. Both particles led to comparable immune responses in mice. The immune responses for MenB (serum bactericidal activity and antibody titers) were equivalent to the control of aluminum hydroxide. For the gp140, the LTK63 was necessary for high titers. Both nanoparticles and microparticles are promising delivery systems.
authors.affiliation: Novartis Vaccines and Diagnostics, Inc., Emeryville, CA 94608, USA.
authors.forename: Janet
authors.initials: J
authors.lastname: Wendorf
authors.forename: James
authors.initials: J
authors.lastname: Chesko
authors.forename: Jina
authors.initials: J
authors.lastname: Kazzaz
authors.forename: Mildred
authors.initials: M
authors.lastname: Ugozzoli
authors.forename: Michael
authors.initials: M
authors.lastname: Vajdy
authors.forename: Derek
authors.initials: D
authors.lastname: O'Hagan
authors.forename: Manmohan
authors.initials: M
authors.lastname: Singh
mesh_terms: D000276:Adjuvants, Immunologic / Q000737:chemistry; D000818:Animals; D000838:Anions / Q000008:administration & dosage; D000942:Antigens, Bacterial / Q000008:administration & dosage / Q000378:metabolism; D002626:Chemistry, Pharmaceutical; D016503:Drug Delivery Systems; D019344:Lactic Acid / Q000008:administration & dosage / Q000737:chemistry*; D051379:Mice; D008863:Microspheres; D053758:Nanoparticles / Q000008:administration & dosage*; D038541:Neisseria meningitidis, Serogroup B / Q000737:chemistry / Q000276:immunology; D011100:Polyglycolic Acid / Q000008:administration & dosage / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D054299:env Gene Products, Human Immunodeficiency Virus / Q000008:administration & dosage* / Q000737:chemistry / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000838:Anions; D000942:Antigens, Bacterial; D054299:env Gene Products, Human Immunodeficiency Virus; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid