C/EBP and C-Myb sites are important for the functional activity of the human myeloperoxidase upstream enhancer.
Myeloperoxidase (MPO), an enzyme active against bacterial and fungal infections, is expressed specifically in myeloblasts and promyelocytes and minimal in other cell types. We recently identified and partially characterized an upstream enhancer located between -4100 and -3844 bp of the MPO gene. We showed that an AML1 site contributes to enhancer activity and specificity. We now demonstrate three additional footprints within the MPO enhancer and provide evidence that C/EBP and c-Myb sites contribute to its functional, tissue-specific activity. This distal enhancer appears to play an important role in the control of MPO transcription during differentiation of myeloid cells.
Yao
Congjun
C
Pathology and Laboratory Medicine Service, Veterans Affairs Medical Center, Decatur, GA 30033, USA.
Qin
Zhenyu
Z
Works
Kimberly N
KN
Austin
Garth E
GE
Young
Andrew N
AN
D001665:Binding Sites; D022762:CCAAT-Enhancer-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D003850:Deoxyribonuclease I / Q000737:chemistry; D004742:Enhancer Elements, Genetic; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D006801:Humans; D009195:Peroxidase / Q000235:genetics*; D023302:Protein Footprinting; D020598:Proto-Oncogene Proteins c-myb / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D022762:CCAAT-Enhancer-Binding Proteins; D020598:Proto-Oncogene Proteins c-myb; D009195:Peroxidase; D003850:Deoxyribonuclease I