title: A large discoidal lipoprotein present in only one of two closely related crayfish.
abstract: The hemolymph lipoproteins of two European freshwater crayfish, Astacus astacus and Astacus leptodactylus, were isolated and characterized. The former species possesses two sex-independent lipoproteins, which can be related to the formerly described high-density lipoprotein (HDL)/beta-glucan binding protein and very high-density lipoprotein/clotting protein from other crustaceans. The latter species, however, contains an additional third lipoprotein with a unique structure. It is a large discoidal HDL with a diameter of 42 nm, a thickness of 7 nm and a density of 1.1 g/ml. SDS-PAGE revealed two different apolipoproteins with molecular masses of 240 and 85 kDa, respectively, arranged in a 1:1 stoichiometry as judged from cross linking experiments. The lipid content of this lipoprotein was 67%, far higher than in every other crustacean lipoprotein described so far. The native molecular mass of this HDL-type lipoprotein was estimated to be about 930 kDa. The lipid content of the other lipoproteins ranged between 25 and 30% for the HDL/beta-glucan binding protein and 6-8% for the VHDL/clotting protein.
authors.affiliation: Institute of Zoology, Johannes Gutenberg-University, Johannes von Müllerweg 6, Mainz, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Stieb
authors.forename: Ulrich
authors.initials: U
authors.lastname: Hoeger
authors.forename: Sven
authors.initials: S
authors.lastname: Schenk
mesh_terms: D000818:Animals; D003400:Astacoidea / Q000737:chemistry*; D002850:Chromatography, Gel; D003432:Cross-Linking Reagents / Q000737:chemistry; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006031:Glycosylation; D006458:Hemolymph / Q000737:chemistry*; D037102:Lectins / Q000737:chemistry; D008055:Lipids / Q000032:analysis; D008074:Lipoproteins / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry / Q000302:isolation & purification; D008297:Male; D008854:Microscopy, Electron; D008970:Molecular Weight; D012737:Sex Factors; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D003432:Cross-Linking Reagents; D037102:Lectins; D008055:Lipids; D008074:Lipoproteins; D008075:Lipoproteins, HDL; C034366:discoidal lipoproteins; C026277:very high density lipoproteins