Porcine rotavirus bearing an aberrant gene stemming from an intergenic recombination of the NSP2 and NSP5 genes is defective and interfering.
Serial undiluted passage of a porcine rotavirus in MA104 cells yielded three distinct virus populations, each of which bore different rearranged genes. Sequencing revealed that each of two populations bore a distinct intragenic recombinant NSP3 gene consisting of a partial duplication in a head-to-tail orientation without altering the NSP3 open reading frame and the third population carried both an intragenic recombinant NSP3 gene and an intergenic recombinant gene (1,647 nucleotides in length) which contained a truncated NSP2 gene inserted into the NSP5 gene at residue 332. The former two populations were viable, whereas the latter population was defective and interfering.
Cao
Dianjun
D
Epidemiology Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Barro
Mario
M
Hoshino
Yasutaka
Y
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D005814:Genes, Viral; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016601:RNA-Binding Proteins / Q000235:genetics*; D011995:Recombination, Genetic; D012401:Rotavirus / Q000235:genetics*; D013552:Swine; D017361:Viral Nonstructural Proteins / Q000235:genetics*
C434828:NSP5 protein, rotavirus group A; D016601:RNA-Binding Proteins; D017361:Viral Nonstructural Proteins; C079234:NS35 protein, rotavirus