title: [Cloning of PD-1 gene and its prokaryotic expression in Escherichia coli].
abstract: OBJECTIVE
  To clone human PD-1 gene, construct a prokaryotic expression plasmid and express in E. coli.
  
  
  METHODS
  The human PD-1 cDNA was cloned by RT-PCR from the total RNA, which was extracted from peripheral blood lymphocyte cell of the patient with chronic hepatitis B. Recombinant PD-1 protein was been expressed and purified after the prokaryotic expression plasmid had been constructed. It was identified by SDS-PAGE, DNA sequencing and amino acid sequencing.
  
  
  RESULTS
  The PD-1 gene was cloned and confirmed by DNA sequencing. The recombinant protein was expressed in E. coli. The purified protein was obtained, then been confirmed by amino acid sequencing.
  
  
  CONCLUSIONS
  The human PD-1 gene was successfully cloned and expressed in E. coli, which lays the foundation for further study on the function and application of PD-1.
authors.affiliation: Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
authors.forename: Si-yong
authors.initials: SY
authors.lastname: Chen
authors.forename: Kun-ping
authors.initials: KP
authors.lastname: Guan
authors.forename: Min-zhuo
authors.initials: MZ
authors.lastname: Guo
authors.forename: Yao
authors.initials: Y
authors.lastname: Yi
authors.forename: Zhi-yuan
authors.initials: ZY
authors.lastname: Jia
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.forename: Yu
authors.initials: Y
authors.lastname: Guo
authors.forename: Sheng-li
authors.initials: SL
authors.lastname: Bi
mesh_terms: D000595:Amino Acid Sequence; D015703:Antigens, CD / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D016133:Polymerase Chain Reaction; D061026:Programmed Cell Death 1 Receptor; D011387:Prokaryotic Cells / Q000378:metabolism*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D015703:Antigens, CD; D051017:Apoptosis Regulatory Proteins; C105992:PDCD1 protein, human; D061026:Programmed Cell Death 1 Receptor