title: Organelle proteomics.
abstract: This unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided. Specific protocols for the isolation of nuclei from various sources (cell cultures, tissues) are also provided.
authors.affiliation: CEA Grenoble, Grenoble, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Lescuyer
authors.affiliation: CEA Grenoble, Grenoble, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Chevallet
authors.affiliation: CEA Grenoble, Grenoble, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Luche
authors.affiliation: CEA Grenoble, Grenoble, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Rabilloud
mesh_terms: D002458:Cell Fractionation; D002478:Cells, Cultured; D015388:Organelles / Q000378:metabolism*; D040901:Proteomics