Loading neurons with dextran-conjugated calcium indicators in intact nervous tissue.
This unit describes methods for filling populations of neurons and their processes, including presynaptic terminals, with dextran-conjugated calcium indicators in central nervous tissue of mammals and lower vertebrates. Techniques for filling neurons in vivo for subsequent analysis either in vivo or in brain slices or en bloc preparations are described. These methods are also suitable for staining neurons in acute and organotypic brain slices.
Delaney
Kerry R
KR
Simon Fraser University, Burnaby, British Columbia, Canada.
D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000737:chemistry; D003911:Dextrans / Q000032:analysis* / Q000737:chemistry; D006651:Histocytochemistry / Q000379:methods*; D007202:Indicators and Reagents / Q000032:analysis; D008856:Microscopy, Fluorescence / Q000379:methods; D009417:Nerve Tissue / Q000737:chemistry / Q000502:physiology; D009474:Neurons / Q000737:chemistry* / Q000502:physiology
D003911:Dextrans; D007202:Indicators and Reagents; D002118:Calcium