Degradation of quinoline by Rhodococcus sp. QL2 isolated from activated sludge.
A novel aerobic gram-positive bacterial strain capable of utilizing quinoline as sole source of carbon, nitrogen and energy was isolated from activated sludge of a coke plant wastewater treatment process. The isolate was identified as Rhodococcus sp. QL2 based on its morphology, physiochemical properties in addition to the results from 16S rDNA sequence analysis. The optimum temperature and the pH for its growth were 35-40 degrees C and 8.0, respectively. Extra nitrogen sources stimulated the bacterial growth on quinoline. Strain QL2 had strong quinoline degradability, and its degradation kinetics could be described with Haldane's model. Strain QL2 also had a broad range of substrate utilization. Identification of intermediates by GC/MS showed Rhodococcus sp. QL2 degraded quinoline via two pathways simultaneously.
Zhu
Shun-Ni
SN
The Key Laboratory of Environmental and Urban Sciences, Shenzhen Graduate School of Peking University, Shenzhen 518055, PR China.
Liu
Dong-Qi
DQ
Fan
Li
L
Ni
Jin-Ren
JR
D001673:Biodegradation, Environmental; D002851:Chromatography, High Pressure Liquid; D003470:Culture Media; D004269:DNA, Bacterial / Q000096:biosynthesis / Q000235:genetics; D005163:Factor Analysis, Statistical; D008401:Gas Chromatography-Mass Spectrometry; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009584:Nitrogen / Q000737:chemistry; D010641:Phenotype; D010802:Phylogeny; D011804:Quinolines / Q000737:chemistry*; D012240:Rhodococcus / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D012722:Sewage / Q000032:analysis*; D013696:Temperature
D003470:Culture Media; D004269:DNA, Bacterial; D011804:Quinolines; D012722:Sewage; D009584:Nitrogen