Metabolic engineering of noviose: heterologous expression of novWUS and generation of a new hybrid antibiotic, noviosylated 10-deoxymethynolide/narbonolide, from Streptomyces venezuelae YJ003-OTBP1.
NovW, novU and novS genes have been characterized as dTDP-4-keto-6-deoxy-D-glucose 3-epimerase, C-5 methyltransferase and dTDP-glucose 4-ketoreductase, respectively involved in noviose biosynthetic pathway. We have cloned and expressed the Streptomyces spheroids novWUS genes in S. venezuelae YJ003-OTBP1. This established the function of novWUS and, at the same time, it also proved that the noviosyl derivative of 10-deoxymethynolide(2)/narbonolide(4) obtained from S. venezuelae YJ003-OTBP1 is a novel hybrid antibiotic.
Pageni
Binod Babu
BB
Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, Sun Moon University, #100, Kalsan-ri, Tangjeong-myeon, Asan-si, Chungnam 336-708, Republic of Korea.
Oh
Tae-Jin
TJ
Lee
Hei Chan
HC
Sohng
Jae Kyung
JK
D000900:Anti-Bacterial Agents / Q000096:biosynthesis; D001709:Biotechnology / Q000379:methods*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013058:Mass Spectrometry; D008826:Microbial Sensitivity Tests; D009005:Monosaccharides / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D013302:Streptomyces / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D018942:Macrolides; D009005:Monosaccharides; C517337:narbonolide; C494073:noviose; C079477:10-deoxymethynolide