title: Use of commercial cards for freeing DNA from mycobacterial strains.
abstract: A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.
authors.affiliation: Regional Reference Center for Mycobacteria, Careggi Hospital, Florence, Italy. enrico.tortoli@unifi.it
authors.forename: Enrico
authors.initials: E
authors.lastname: Tortoli
authors.forename: Serena
authors.initials: S
authors.lastname: Baruzzo
authors.forename: Stefania
authors.initials: S
authors.lastname: Lauria
authors.forename: Alessandro
authors.initials: A
authors.lastname: Mariottini
mesh_terms: D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D009161:Mycobacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D011933:Reagent Kits, Diagnostic; D017422:Sequence Analysis, DNA; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*
chemical_list: D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic