[Molecular characterization of hantavirus Zhejiang isolate ZT10 strain from M. fartis].
OBJECTIVE
To learn about the complete genomic sequence of the Seoul virus strain ZT10 isolated from M. fartis.


METHODS
The total RNA was extracted from the infected Vero E6 cells and amplified by RT-PCR. The purified PCR products were cloned into T-vector and sequenced.


RESULTS
The results demonstrated that the complete genome of ZT10 was comprised of L(6530), M(3651) and S(1753) segments which encoded 2151-1133 and 429 amino acids respectively.


CONCLUSIONS
Analysis of sequence revealed that the ZT10 belonged to Seoul virus. The nucleotide sequence identity of the M gene with Seoul virus was 84.0%-96.3%. The identity with Hantan vrisu (Prospect Hill virus, Tula virus) isolated from M. fartis was 57.5%-60.9%. The sequence identity of the S gene with Seoul virus was 87.9%-96.0% at nucleotide level and 96.9%-97.9% at amino acid level.
Weng
Jing-qing
JQ
Zhejiang Provincial Center for Disease Prevention and Control, Hangzhou 310009, China. Jingqingw@yahoo.com.cn
Xie
Rong-hui
RH
Yao
Ping-ping
PP
Li
Ming-hong
MH
Xu
Fang
F
Lu
Yi-yu
YY
Zhu
Han-ping
HP
Cui
Qing-rong
QR
Zhu
Z Y
ZY
D000818:Animals; D000956:Antigens, Viral / Q000032:analysis; D002460:Cell Line; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D019085:Fluorescent Antibody Technique, Direct; D006476:Orthohantavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D000956:Antigens, Viral; D018076:DNA, Complementary; D004279:DNA, Viral