title: An instrument for automated purification of nucleic acids from contaminated forensic samples.
abstract: Forensic crime scene sample analysis, by its nature, often deals with samples in which there are low amounts of nucleic acids, on substrates that often lead to inhibition of subsequent enzymatic reactions such as PCR amplification for STR profiling. Common substrates include denim from blue jeans, which yields indigo dye as a PCR inhibitor, and soil, which yields humic substances as inhibitors. These inhibitors frequently co-extract with nucleic acids in standard column or bead-based preps, leading to frequent failure of STR profiling. We present a novel instrument for DNA purification of forensic samples that is capable of highly effective concentration of nucleic acids from soil particulates, fabric, and other complex samples including solid components. The novel concentration process, known as SCODA, is inherently selective for long charged polymers such as DNA, and therefore is able to effectively reject known contaminants. We present an automated sample preparation instrument based on this process, and preliminary results based on mock forensic samples.
authors.forename: David J
authors.initials: DJ
authors.lastname: Broemeling
authors.forename: Joel
authors.initials: J
authors.lastname: Pel
authors.forename: Dylan C
authors.initials: DC
authors.lastname: Gunn
authors.forename: Laura
authors.initials: L
authors.lastname: Mai
authors.forename: Jason D
authors.initials: JD
authors.lastname: Thompson
authors.forename: Hiron
authors.initials: H
authors.lastname: Poon
authors.forename: Andre
authors.initials: A
authors.lastname: Marziali