title: Introduction of large insert DNA into mammalian cells and embryos.
abstract: This unit provides a set of protocols for introducing large insert DNA into cultured mammalian cells and embryos. Two different methods, spheroplast fusion and lipofection, are described for effecting transfer of YACs or gel-purified YAC DNA into cells. Additional protocols discuss preparing and transferring BACs into cells by lipofection and into embryos by microinjection.
authors.affiliation: Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.
authors.forename: R H
authors.initials: RH
authors.lastname: Reeves
authors.forename: D E
authors.initials: DE
authors.lastname: Cabin
authors.forename: B
authors.initials: B
authors.lastname: Lamb
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D018244:Chromosomes, Artificial, Yeast / Q000235:genetics; D017931:DNA Primers; D004274:DNA, Recombinant / Q000008:administration & dosage* / Q000235:genetics*; D004622:Embryo, Mammalian / Q000166:cytology; D018014:Gene Transfer Techniques; D005819:Genetic Markers; D005826:Genetics, Medical; D006801:Humans; D008561:Membrane Fusion; D051379:Mice; D008845:Microinjections; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D013104:Spheroplasts / Q000235:genetics
chemical_list: D017931:DNA Primers; D004274:DNA, Recombinant; D005819:Genetic Markers