Disruption of the Plasmodium berghei 2-Cys peroxiredoxin TPx-1 gene hinders the sporozoite development in the vector mosquito.
To investigate the physiologic role of cytosolic 2-Cys peroxiredoxin of Plasmodium berghei (PbTPx-1), we infected the vector mosquito Anopheles stephensi with a parasite carrying a targeted knockout of pbtpx-1 (Prx-KO). The number of Prx-KO midgut oocysts at 14-15 days post-feeding (pf) was comparable to that of the parent strain (WT); however, the numbers of sporozoites that formed in midgut oocysts and accumulated in the salivary gland of Prx-KO-infected mosquitoes by 21 days pf were decreased to 10-20% and 3-10%, respectively, of those values in WT-infected mosquitoes. A higher frequency of DNA strand breaks was detected in Prx-KO oocysts than in WT oocysts. Sporozoites carrying the targeted disruption had reduced infectivity in mice; however, the knockout did not affect the ability of the sporozoite to reach the liver parenchyma and initiate exo-erythrocytic form (EEF) development. TPx-1 may be involved in development during exponentially multiplying stages, such as sporozoites and EEF.
Yano
Kazuhiko
K
Research Institute, International Medical Center of Japan, Tokyo, Japan.
Otsuki
Hitoshi
H
Arai
Meiji
M
Komaki-Yasuda
Kanako
K
Tsuboi
Takafumi
T
Torii
Motomi
M
Kano
Shigeyuki
S
Kawazu
Shin-Ichiro
S
D000818:Animals; D009033:Culicidae / Q000469:parasitology*; D053960:DNA Breaks; D016054:DNA, Protozoan / Q000235:genetics; D041981:Gastrointestinal Tract / Q000737:chemistry / Q000469:parasitology; D017353:Gene Deletion; D008099:Liver / Q000469:parasitology; D008288:Malaria / Q000469:parasitology*; D051379:Mice; D016254:Mutagenesis, Insertional; D010270:Parasite Egg Count; D054464:Peroxiredoxins / Q000235:genetics / Q000502:physiology*; D010962:Plasmodium berghei / Q000201:enzymology / Q000254:growth & development* / Q000472:pathogenicity*; D015800:Protozoan Proteins / Q000235:genetics / Q000502:physiology*; D012469:Salivary Glands / Q000469:parasitology; D034101:Sporozoites / Q000201:enzymology / Q000254:growth & development
D016054:DNA, Protozoan; D015800:Protozoan Proteins; D054464:Peroxiredoxins