Single tube optimisation of APOE genotyping based on melting curve analysis.
OBJECTIVE
To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes.


METHODS
We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that takes advantage of the different probe wavelength emissions.


RESULTS
Our method offers high quality outcomes, minimum interferences between probe signals and is 100% concordant with the reference protocol.


CONCLUSIONS
This method is cheaper, faster and more reliable and versatile than other alternatives proposed.
Francés
Francesc
F
Genetic and Molecular Epidemiology Unit, Department of Preventive Medicine, School of Medicine. University of Valencia, Valencia, Spain. francesc.frances@uv.es
Portolés
Olga
O
Sorlí
José Vicente
JV
Guillén
Marisa
M
González
José I
JI
Corella
Dolores
D
D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics*; D002318:Cardiovascular Diseases / Q000097:blood / Q000235:genetics; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D013816:Thermodynamics
D001057:Apolipoproteins E; D017931:DNA Primers