Interaction trap/two-hybrid system to identify interacting proteins.
The yeast two-hybrid method (or interaction trap) is a powerful technique for detecting protein interactions. The procedure is performed using transcriptional activation of a dual reporter system in yeast to identify interactions between a protein of interest (the bait protein) and the candidate proteins for interaction. The method can be used to screen a protein library for interactions with a bait protein or to test for association between proteins that are expected to interact based on prior evidence. Interaction mating facilitates the screening of a library with multiple bait proteins.
Golemis
Erica A
EA
Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.
Serebriiskii
Ilya
I
Finley
Russell L
RL
Kolonin
Mikhail G
MG
Gyuris
Jeno
J
Brent
Roger
R
D035843:Biomedical Research / Q000379:methods; D019151:Peptide Library; D025941:Protein Interaction Mapping / Q000379:methods; D020798:Two-Hybrid System Techniques
D019151:Peptide Library