Purification of alpha-galactosidase and invertase by three-phase partitioning from crude extract of Aspergillus oryzae.
Alpha-galactosidase and invertase were accumulated in a coherent middle phase in a three-phase partitioning system under different conditions (ammonium sulphate, ratio of tert-butanol to crude extract, temperature and pH). Alpha-galactosidase and invertase were purified 15- and 12-fold with 50 and 54% activity recovery, respectively. The fractions of interfacial precipitate arising from the three-phase partitioning were analyzed by SDS-PAGE. Both purified preparations showed electrophoretic homogeneity on SDS-PAGE.
Dhananjay
S K
SK
Department of Biochemistry, Gulbarga University, Gulbarga, Karnataka, India.
Mulimani
V H
VH
D001236:Aspergillus oryzae / Q000201:enzymology*; D001671:Biochemistry / Q000379:methods*; D011232:Chemical Precipitation; D004591:Electrophoresis, Polyacrylamide Gel; D000519:alpha-Galactosidase / Q000032:analysis / Q000302:isolation & purification*; D043324:beta-Fructofuranosidase / Q000032:analysis / Q000302:isolation & purification*
D000519:alpha-Galactosidase; D043324:beta-Fructofuranosidase