title: Up-regulated HIF-1alpha is involved in the hypoxic tolerance induced by hyperbaric oxygen preconditioning.
abstract: Hyperbaric oxygen preconditioning (HBO-PC) has been shown to be effective in preventing hypoxic injuries in many animal models. The aim of the present study was to examine the hypoxic tolerance induced by HBO-PC and to explore the role of hypoxia-inducible factor-1alpha (HIF-1alpha) in a global hypoxia model. Male mice received HBO-PC before hypoxia exposure and swimming. HBO-PC significantly prolonged the survival time and the tolerance time of swimming under normobaric hypoxia. HBO-PC increased the protein content of HIF-1alpha and erythropoietin (EPO) in the cerebral cortex and hippocampus and prevented the changes of blood brain barrier (BBB) permeability and brain edema caused by hypoxia exposure. The results suggested that HBO-PC induced hypoxic tolerance in mice via up-regulation of HIF-1alpha and its downstream genes.
authors.affiliation: Department of Diving Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, 200433, PR China.
authors.forename: Zhaoyun
authors.initials: Z
authors.lastname: Peng
authors.forename: Ping
authors.initials: P
authors.lastname: Ren
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Kang
authors.forename: Jing
authors.initials: J
authors.lastname: Du
authors.forename: Qinglin
authors.initials: Q
authors.lastname: Lian
authors.forename: Yun
authors.initials: Y
authors.lastname: Liu
authors.forename: John H
authors.initials: JH
authors.lastname: Zhang
authors.forename: Xuejun
authors.initials: X
authors.lastname: Sun
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001522:Behavior, Animal; D001929:Brain Edema / Q000209:etiology; D002540:Cerebral Cortex / Q000378:metabolism; D004195:Disease Models, Animal; D004921:Erythropoietin / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism; D006931:Hyperbaric Oxygenation; D000860:Hypoxia / Q000150:complications / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics / Q000378:metabolism*; D019194:Ischemic Preconditioning; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D016019:Survival Analysis; D013997:Time Factors; D015854:Up-Regulation / Q000502:physiology*
chemical_list: C497443:Hif1a protein, mouse; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D004921:Erythropoietin