Bacteria detection utilizing electrical conductivity.
Real-time and specific detection of single bacterium remains a fundamental challenge and draws very much attention. Using test patterns composed of interdigitated Au-electrode arrays modified with antibody, the specific and quantitative detection of the electrical conductivity of a single Escherichia coli (E. coli, JM109) has been carried out in this work. The key is to ensure low background current of the antibody-modified test patterns before bacteria detection (<0.7pA in this case) and minimize the residual moisture or hydration after E. coli immobilization, such as via the use of 1-min bake at 50 degrees C prior to electrical measurement. This method holds great potential for future application in the real-time, specific, and quantitative bacterium detection down to a single bacterium cell.
Lu
Yi-Chun
YC
Department of Materials Science and Engineering, National Tsing-Hua University, 101, Sec 2, Kuang-Fu Road, Hsinchu 30013, Taiwan.
Chuang
Ya-Shuan
YS
Chen
Yi-Yang
YY
Shu
An-Chi
AC
Hsu
Hsing-Yu
HY
Chang
Hwan-You
HY
Yew
Tri-Rung
TR
D001681:Biological Assay / Q000295:instrumentation*; D015169:Colony Count, Microbial / Q000295:instrumentation*; D004553:Electric Conductivity; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D004926:Escherichia coli / Q000302:isolation & purification*