[Changes of pulmonary surfactant protein A in young rats with acute lung injury induced by lipopolysaccharide].
OBJECTIVE
Pulmonary surfactant protein A (SP-A) plays an important role in the maintenance of pulmonary surfactant function and innative immune defence. This study aimed to explore the changes of SP-A concentration in the lungs of young rats with acute lung injury.


METHODS
Sprague-Dawley rats were randomly assigned to control and lung injury groups. Acute lung injury was induced by intraperitoneal injection of lipopolysaccharide (LPS) (4 mg/kg) in the lung injury group. The same amount of normal saline was given for the control group. The two groups were subdivided into 6 groups sacrificed at 6, 12, 24, 36, 48 and 72 hrs of injection (n=8 each). Western blot was employed to detect SP-A concentration in the lung tissues.


RESULTS
SP-A concentration in the lung injury group was not different from the the control group within 12 hrs after LPS injection. SP-A concentration in the lung injury group was elevated significantly during 24-48 hrs after LPS injection, peaking at 36 hrs (6.94+/-0.80 vs 5.01+/-0.36; P< 0.01), compared with the controls. However, SP-A concentration in the lung injury group was significantly reduced 72 hrs after LPS injection compared with the controls (P< 0.01).


CONCLUSIONS
The changes of lung SP-A concentration in rats following acute lung injury were time-dependent. The transient elevation of SP-A concentration in the lungs indicated a strong compensation ability of SP-A in the host defence against acute lung injury.
Shu
Lin-Hua
LH
Department of Pediatrics, Shengjing Hospital of China Medical University, Shenyang 110004, China. shulinhua@126.com
Wei
Ke-Lun
KL
Liu
Chun-Feng
CF
Han
Xiao-Hua
XH
Shang
Yun-Xiao
YX
Cai
Xu-Xu
XX
Li
Jiu-Jun
JJ
Wang
Li-Jie
LJ
D000818:Animals; D005260:Female; D008070:Lipopolysaccharides / Q000633:toxicity*; D008168:Lung / Q000737:chemistry*; D008297:Male; D037662:Pulmonary Surfactant-Associated Protein A / Q000032:analysis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012128:Respiratory Distress Syndrome / Q000378:metabolism*
D008070:Lipopolysaccharides; D037662:Pulmonary Surfactant-Associated Protein A