title: Bacterial adherence to SiO2-based multifunctional bioceramics.
abstract: The bacterial adherence onto different multifunctional silica-based bioceramics has been evaluated. Staphylococcus aureus and Staphylococcus epidermidis were chosen, as they cause the majority of the implant-related infections in this field. Two SiO2 mesoporous materials (MCM-41, SBA-15), an ordered SiO2-CaO-P2O5 mesoporous glass (OMG), and a biphasic magnetic bioceramic (BMB), were incubated with S. aureus and S. epidermidis for 90 min, and subsequently sonicated to quantify the number of adhered bacteria on each material. It was found that S. aureus and S. epidermidis (10(8) CFU/mL) adhered significantly less to BMB samples when compared to MCM-41, SBA-15, or OMG. However, when the material pores accessible for bacteria in each material were taken into account, the lowest bacterial adherence was found in MCM-41, and the highest in SBA-15. The results show that bacterial adherence is higher on mesoporous bioceramics, although this higher microbial attachment is mainly due to the intergranular porosity and grain size morphology rather than to the mesoporous structure.
authors.affiliation: Department of Clinical Microbiology, Fundación Jiménez Díaz-UTE, Avda. de Reyes Católicos 2, ES-28040 Madrid, Spain.
authors.forename: Teemu J
authors.initials: TJ
authors.lastname: Kinnari
authors.forename: Jaime
authors.initials: J
authors.lastname: Esteban
authors.forename: Enrique
authors.initials: E
authors.lastname: Gomez-Barrena
authors.forename: Nieves
authors.initials: N
authors.lastname: Zamora
authors.forename: Ricardo
authors.initials: R
authors.lastname: Fernandez-Roblas
authors.forename: Alejandra
authors.initials: A
authors.lastname: Nieto
authors.forename: Juan C
authors.initials: JC
authors.lastname: Doadrio
authors.forename: Adolfo
authors.initials: A
authors.lastname: López-Noriega
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ruiz-Hernández
authors.forename: Daniel
authors.initials: D
authors.lastname: Arcos
authors.forename: María
authors.initials: M
authors.lastname: Vallet-Regí
mesh_terms: D001422:Bacterial Adhesion / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry*; D002118:Calcium / Q000378:metabolism; D002516:Ceramics / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000502:physiology*; D013212:Staphylococcus epidermidis / Q000502:physiology*; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D012822:Silicon Dioxide; D002118:Calcium