title: Rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of clonidine in human plasma.
abstract: A rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the estimation of clonidine in human plasma. Clonidine was extracted from human plasma by using solid-phase extraction technique. Nizatidine was used as the internal standard. A Hypurity C18 (50 mm x 4.6 mm i.d., 5 microm particle size) column provided chromatographic separation of analyte followed by detection with mass spectrometry. The method involves a rapid solid-phase extraction from plasma, simple isocratic chromatography conditions and mass spectrometric detection that enables detection up to picogram levels with a total run time of 3.0 min only. The method was validated over the range of 50-2500 pg/mL. The absolute recoveries for clonidine (71.86%) and IS (69.44%) achieved from spiked plasma samples were consistent and reproducible.
authors.affiliation: Department of Chemistry, Ruia College, Mumbai University, Matunga, Mumbai, India. parekh.sagar@rediffmail.com
authors.forename: Sagar A
authors.initials: SA
authors.lastname: Parekh
authors.forename: Ashutosh
authors.initials: A
authors.lastname: Pudage
authors.forename: Santosh S
authors.initials: SS
authors.lastname: Joshi
authors.forename: Vikas V
authors.initials: VV
authors.lastname: Vaidya
authors.forename: Noel A
authors.initials: NA
authors.lastname: Gomes
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000097:blood*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D003000:Clonidine / Q000097:blood*; D006801:Humans; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000316:Adrenergic alpha-Agonists; D003000:Clonidine