title: Glutamate levels and activity of the T cell voltage-gated potassium Kv1.3 channel in patients with systemic lupus erythematosus.
abstract: OBJECTIVE
  Alterations in glutamate homeostasis and Kv1.3 voltage-gated potassium channel function have been independently associated with T cell dysfunction, whereas selective blockade of Kv1.3 channels inhibits T cell activation and improves T cell-mediated manifestations in animal models of autoimmunity. Because low extracellular glutamate concentrations enhance the activity of this channel in normal T cells ex vivo, we undertook this study to examine serum glutamate concentrations and Kv1.3 channel activity in patients with systemic lupus erythematosus (SLE).
  
  
  METHODS
  We used high-performance liquid chromatography for glutamate measurements, and we used the whole-cell patch-clamp technique for electrophysiologic studies performed in freshly isolated, noncultured peripheral T cells.
  
  
  RESULTS
  Mean +/- SD serum concentrations of glutamate were lower in patients with either clinically quiescent SLE (77 +/- 27 microM [n = 18]) or active SLE (61 +/- 36 microM [n = 16]) than in healthy controls (166 +/- 64 microM [n = 24]) (both P < 0.0001). The intrinsic gating properties of the Kv1.3 channels in lupus T cells were found to be comparable with those in healthy control-derived T cells. Notably, electrophysiologic data from SLE patient-derived T cells exposed to extracellular glutamate concentrations similar to their respective serum levels (50 microM) demonstrated Kv1.3 current responses enhanced by almost 20% (P < 0.01) compared with those subsequently obtained from the same cell in the presence of glutamate concentrations within control serum levels (200 microM).
  
  
  CONCLUSIONS
  Based on the key role of Kv1.3 channel activity in lymphocyte physiology, an enhancing in vivo effect of low serum glutamate concentrations on the functional activity of this channel may contribute to lupus T cell hyperactivity. Studies to further elucidate Kv1.3 responses in SLE, as well as the possible pathogenetic role of this unsuspected metabolic abnormality, may have therapeutic implications for SLE patients.
authors.affiliation: Athens University Medical School, Athens, Greece.
authors.forename: C
authors.initials: C
authors.lastname: Poulopoulou
authors.forename: Z
authors.initials: Z
authors.lastname: Papadopoulou-Daifoti
authors.forename: A
authors.initials: A
authors.lastname: Hatzimanolis
authors.forename: K
authors.initials: K
authors.lastname: Fragiadaki
authors.forename: A
authors.initials: A
authors.lastname: Polissidis
authors.forename: E
authors.initials: E
authors.lastname: Anderzanova
authors.forename: P
authors.initials: P
authors.lastname: Davaki
authors.forename: C G
authors.initials: CG
authors.lastname: Katsiari
authors.forename: P P
authors.initials: PP
authors.lastname: Sfikakis
mesh_terms: D000293:Adolescent; D000328:Adult; D005260:Female; D018698:Glutamic Acid / Q000097:blood*; D006801:Humans; D051664:Kv1.3 Potassium Channel / Q000502:physiology*; D008180:Lupus Erythematosus, Systemic / Q000097:blood* / Q000378:metabolism*; D008875:Middle Aged
chemical_list: D051664:Kv1.3 Potassium Channel; D018698:Glutamic Acid