Assays for the mannan-binding lectin pathway.
This unit contains protocols that can be used to measure mannan-binding lectin (MBL) levels and MBL pathway activity in human plasma or serum. Using a modification of the conventional ELISA, the detection reagent used in the methods described (e.g., an antibody or streptavidin) is labeled with Eu3+ instead of an enzyme. The sensitizing reagent used in these assays employ the use of anti-MBL antibody or mannan. The unit also includes an MBL/MASP complex activity assay to evaluate MBL activity in serum or plasma samples, which allows for the measurement of the specific activity of the MBL pathway.
Gadjeva
Mihaela
M
University of Aarhus, Aarhus, Denmark.
Thiel
Steffen
S
Jensenius
Jens C
JC
D037582:Complement Pathway, Mannose-Binding Lectin / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D037601:Mannose-Binding Lectin / Q000032:analysis* / Q000097:blood / Q000276:immunology; D050606:Mannose-Binding Protein-Associated Serine Proteases / Q000032:analysis* / Q000276:immunology / Q000378:metabolism
D037601:Mannose-Binding Lectin; D050606:Mannose-Binding Protein-Associated Serine Proteases