A new tool for probing of cell-cell communication: human embryonic germ cells inducing apoptosis of SKOV3 ovarian cancer cells on a microfluidic chip.
A microfluidic device with unidirectional perfusion has been developed to observe the effect of human embryonic germ (hEG) cells on SKOV3 cells. The hEG and SKOV3 cells were seeded in the inlet and the outlet reservoirs separately, and co-cultured for 2 days. The medium was perfused unidirectionally from the inlet to the outlet. The growth inhibition of SKOV3 cells was monitored online and the apoptosis signals in SKOV3 culture area decreased along the flow of the medium. In conclusion, microfluidic chip is a potentially useful tool to investigate the effect of stem cells on cancer cells with intuitionistic cell-based screens.
Song
Xinyan
X
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, 107# Wenhua Xi Road, Jinan 250012, P.R. China.
Kong
Beihua
B
Li
Dong
D
D000469:Alkaline Phosphatase / Q000378:metabolism; D017209:Apoptosis; D002450:Cell Communication; D002452:Cell Count; D018929:Cell Culture Techniques; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002999:Clone Cells; D004622:Embryo, Mammalian / Q000166:cytology*; D005260:Female; D005854:Germ Cells / Q000166:cytology*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010051:Ovarian Neoplasms / Q000473:pathology*
D000469:Alkaline Phosphatase