UV laser mediated cell selective destruction by confocal microscopy.
Analysis of cell-cell interactions, cell function and cell lineages greatly benefits selective destruction techniques, which, at present, rely on dedicated, high energy, pulsed lasers and are limited to cells that are detectable by conventional microscopy. We present here a high resolution/sensitivity technique based on confocal microscopy and relying on commonly used UV lasers. Coupling this technique with time-lapse enables the destruction and following of any cell(s) in any pattern(s) in living animals as well as in cell culture systems.
Soustelle
Laurent
L
Institut de Génétique et Biologie Moléculaire et Cellulaire, IGBMC/CNRS/ULP/INSERM - BP 10142 67404 ILLKIRCH, c.u. de Strasbourg, France. lsoustel@titus.u-strasbg.fr
Aigouy
Benoît
B
Asensio
Marie-Laure
ML
Giangrande
Angela
A
D000818:Animals; D030801:Animals, Genetically Modified; D002450:Cell Communication; D018929:Cell Culture Techniques / Q000379:methods*; D015509:Developmental Biology / Q000295:instrumentation / Q000379:methods*; D004330:Drosophila; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D007834:Lasers; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods*; D014466:Ultraviolet Rays
D049452:Green Fluorescent Proteins