title: A mouse model for cytomegalovirus infection.
abstract: This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (mCMV). Methods are included for propagating mCMV in cell cultures and for preparing a more virulent form of mCMV from salivary glands of infected mice. A plaque-forming cell (PFC) assay is provided for measuring mCMV titers of infected tissues or virus stocks. In addition, a method is described for preparing the murine embryonic fibroblasts used for propagating mCMV and for the PFC assay.
authors.affiliation: University of Munich, Max von Pettenkofer Institute, Munich, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Brune
authors.forename: H
authors.initials: H
authors.lastname: Hengel
authors.forename: U H
authors.initials: UH
authors.lastname: Koszinowski
mesh_terms: D000818:Animals; D003586:Cytomegalovirus Infections / Q000821:virology; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000821:virology; D006566:Herpesviridae Infections / Q000821:virology; D051379:Mice; D018146:Muromegalovirus / Q000302:isolation & purification / Q000502:physiology; D012469:Salivary Glands / Q000821:virology*; D019562:Viral Load; D010948:Viral Plaque Assay / Q000379:methods*; D014779:Virus Replication