[Synergistic antileukemic effect of phytoestrogens and chemotherapeutic drugs on leukemic cell lines in vitro].
Natural phytoestrogens such as the isoflavones genistein and daidzein, and the flavones quercetin exhibit anti-cancer properties. This study was purpose to investigate the anti-proliferative effect of phytoestrogens on acute myeloid leukemia (AML) and chronic myeloid leukemia (CML) cells, and their synergistic antileukemic effect in combination with chemotherapeutic drugs. Optimal dosage of genistein, quercetin and in combination with chemicals for leukemia cells were determined by experiments. Cell viability, apoptosis induction and cell cycle arrest were detected by trypan blue staining, MTT assay, optical microscopy, flow cytometry (FCM). The schedule treatment of combination of genistein and chemicals was determined. The results showed that genistein exhibited a dose- and time-dependent inhibitory effect on cell proliferation in NB4 and HL-60 cells, induced apoptosis and cell cycle arrest in G2/M phase. Quercetin had evident inhibitory effect on the proliferation of K562 and K562/A cells. The combination of genistein and chemicals exerted a synergistic effect on cell growth inhibition. In conclusion, this study demonstrated the synergistic antileukemic effect of genistein with chemotherapeutic drugs on leukemic cells. This combination appears to be a new idea for the clinical novel treatment of leukemia.
Shen
Jing
J
Department of Oncology, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
Zhang
Wei-Jing
WJ
Tai
Yan-Chin
YC
Wong
Ching-Ho Stephen
CH
Xie
Zhigang
Z
Chen
Chien-Shing
CS
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D004357:Drug Synergism; D019833:Genistein / Q000494:pharmacology; D018922:HL-60 Cells; D006801:Humans; D007529:Isoflavones / Q000494:pharmacology; D007948:Leukemia, Monocytic, Acute / Q000473:pathology*; D015470:Leukemia, Myeloid, Acute / Q000473:pathology*; D048789:Phytoestrogens / Q000494:pharmacology*; D011794:Quercetin / Q000494:pharmacology
D000970:Antineoplastic Agents; D007529:Isoflavones; D048789:Phytoestrogens; C004742:daidzein; D011794:Quercetin; D019833:Genistein