Adiponectin promotes endothelial progenitor cell number and function.
Obesity-linked diseases are associated with suppressed endothelial progenitor cell (EPC) function. Adiponectin is an adipose-derived protein that is downregulated in obese and diabetic subjects. Here, we investigated the effects of adiponectin on EPCs. EPC levels did not increase in adiponectin deficient (APN-KO) in response to hindlimb ischemia. Adenovirus-mediated delivery of adiponectin increased EPC levels in both WT and APN-KO mice. Incubation of human peripheral blood mononuclear cells with adiponectin led to an increase of the number of EPCs. Adiponectin induced EPC differentiation into network structures and served as a chemoattractant in EPC migration assays. These data suggest that hypoadiponectinemia may contribute to the depression of EPC levels that are observed in patients with obesity-related cardiovascular disorders.
Shibata
Rei
R
Department of Cardiology, Nagoya University Graduate School of Medicine, Nagoya, Japan. rshibata@med.nagoya-u.ac.jp
Skurk
Carsten
C
Ouchi
Noriyuki
N
Galasso
Gennaro
G
Kondo
Kazuhisa
K
Ohashi
Taiki
T
Shimano
Masayuki
M
Kihara
Shinji
S
Murohara
Toyoaki
T
Walsh
Kenneth
K
D000256:Adenoviridae; D052242:Adiponectin / Q000235:genetics / Q000502:physiology*; D000818:Animals; D002452:Cell Count; D002454:Cell Differentiation / Q000235:genetics; D002465:Cell Movement; D042783:Endothelial Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D051379:Mice; D018345:Mice, Knockout; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection
D052242:Adiponectin