title: Expression, purification, and characterization of aspartic endopeptidases: Plasmodium plasmepsins and "short" recombinant human pseudocathepsin.
abstract: The unit describes a basic protocols utilized to obtain milligram amounts of enzymatically active, pure recombinant Plasmodium plasmepsins and "short" human pseudocathepsin D. Specific details for the expression and purification of Plasmodium falciparum plasmepsin 2 and "short" human pseudocathepsin D in zymogen form are described in this chapter. The plasmepsin 2 protocols are also applicable to Plasmodium vivax, P. ovale, and P. malariae plasmepsins, as well as P. falciparum plasmepsin 4.
authors.affiliation: University of Florida, Gainesville, Florida.
authors.forename: Bret B
authors.initials: BB
authors.lastname: Beyer
authors.affiliation: University of Florida, Gainesville, Florida.
authors.forename: Nathan E
authors.initials: NE
authors.lastname: Goldfarb
authors.affiliation: University of Florida, Gainesville, Florida.
authors.forename: Ben M
authors.initials: BM
authors.lastname: Dunn
mesh_terms: D000818:Animals; D016282:Aspartic Acid Endopeptidases / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D002402:Cathepsin D / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006801:Humans; D010961:Plasmodium / Q000201:enzymology*; D011498:Protein Precursors / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D011498:Protein Precursors; D011994:Recombinant Proteins; D016282:Aspartic Acid Endopeptidases; C089643:plasmepsin; D002402:Cathepsin D