[Isolation, characterization and fermentation condition of coenzyme Q10 producing strain with solanesol as precursor].
A bacterium capable of producing Coenzyme Q10 (CoQ10, was isolated from soil. Based on analysis of the 16S rDNA sequence, traditional physiological characteristics, and Biolog-GN, the strain was belonging to the genus Sphingomonas and named as Sphingomonas sp. ZUTEO3. The optimum fermentation condition of CoQ10 production was as following: glucose 15 g/L, (NH4)2SO4 10 g/L, original pH 8.0 and at 25 degrees C. The addition of solanesol could improve CoQ10 production. The optimal condition for the bioconservation from solanesol to CoQ10 was as following: adding 0.75 g/L of raw solanesol after the first 12 h fermentation and continued for another 12 h fermentation. The maximal yield of CoQ10 reached 96.88 mg/L.
Liu
Huagui
H
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China. liuhuagui1111@163.com
Fang
Jianjun
J
Jin
Ling
L
Zhong
Weihong
W
Ye
Zijuan
Z
D018533:Biomass; D003470:Culture Media / Q000378:metabolism; D046508:Culture Techniques; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005285:Fermentation; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D012988:Soil Microbiology; D020578:Sphingomonas / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D013729:Terpenes / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000378:metabolism
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D013729:Terpenes; D014451:Ubiquinone; C024989:coenzyme Q10; C017719:solanesol