Purification of CD45+ hematopoietic cells directly from human bone marrow using a flow-based P-selectin-coated microtube.
Recently, a flow-based selectin-dependent method for capture and enrichment of specific type blood cells (CD34+ hematopoietic stem and progenitor cells) from bone marrow and peripheral blood has been described. Using a similar approach, here we show isolation of CD45+ blood cells directly from human bone marrow to a high purity (90%-97%). The process mimics a ubiquitous mechanism of cell trafficking in the body for the recruitment of neutrophils during inflammation. The method is straightforward, rapid, and may represent a practical alternative to CD45+ cell enrichment procedures required for chimerism analysis following allogenic stem-cell transplantation.
Narasipura
Srinivas D
SD
Department of Biomedical Engineering, University of Rochester, Rochester, New York, USA.
Wojciechowski
Joel C
JC
Duffy
Brian M
BM
L Liesveld
Jane
J
King
Michael R
MR
D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D002469:Cell Separation / Q000379:methods*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D017493:Leukocyte Common Antigens; D007962:Leukocytes / Q000166:cytology; D008722:Methods; D019007:P-Selectin / Q000378:metabolism; D010477:Perfusion; D011485:Protein Binding
D019007:P-Selectin; D017493:Leukocyte Common Antigens