Metabolism of prebiotic products containing beta(2-1) fructan mixtures by two Lactobacillus strains.
The capacity of two probiotic strains, isolated from human breast milk, to use several beta(2-1) fructan mixtures as carbon and energy source in in vitro cultures has been tested. Results showed that both strains, Lactobacillus gasseri CECT5714 and Lactobacillus fermentum CECT5716, reached higher growth levels on culture media containing fructooligosaccharide mixtures produced by enzymatic synthesis, compared to those obtained by inulin hydrolysis. Furthermore, the shortest beta(2-1) fructan, kestose, was the only prebiotic compound in the mixtures significantly metabolized in all growth media tested. Analysis of short-chain fatty acid production showed no correlation between the fatty acid profile produced and the carbon source used in each experiment. These data could serve to select appropriate beta(2-1) fructans to be used as prebiotics for L. gasseri CECT5714 and L. fermentum CECT5716 and to design suitable symbiotic food products containing the mentioned lactobacilli.
Bañuelos
Oscar
O
Department of Biotechnology, Puleva Biotech S.A., Camino de Purchil 66, 18004 Granada, Spain. obanuelos@pulevabiotech.es
Fernández
Layla
L
Corral
José M
JM
Valdivieso-Ugarte
Magdalena
M
Adrio
José L
JL
Velasco
Javier
J
D003470:Culture Media; D005260:Female; D005630:Fructans / Q000378:metabolism*; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D052197:Limosilactobacillus fermentum / Q000254:growth & development / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D019936:Probiotics
D003470:Culture Media; D005630:Fructans; D009844:Oligosaccharides; C120489:oligofructose