title: Phage display for generating peptide reagents.
abstract: This unit presents detailed protocols for selection and propagation of landscape phages, which are fusions of filamentous phage fd (or its close relatives M13 and f1) and foreign DNA that result in chimeric phage virions with foreign peptides (8 to 9 amino acids long) covering the entire surface of the phage particles. These landscape phages bind specifically to mammalian and bacterial cells, spores, or discrete molecular targets.
authors.affiliation: Division of Natural Sciences, Maryville College, Maryville, Tennessee, USA.
authors.forename: Jennifer R
authors.initials: JR
authors.lastname: Brigati
authors.forename: Tatiana I
authors.initials: TI
authors.lastname: Samoylova
authors.forename: Prashanth K
authors.initials: PK
authors.lastname: Jayanna
authors.forename: Valery A
authors.initials: VA
authors.lastname: Petrenko
mesh_terms: D000818:Animals; D001435:Bacteriophages / Q000235:genetics*; D007202:Indicators and Reagents / Q000737:chemistry*; D008322:Mammals; D015335:Molecular Probes; D010455:Peptides / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents; D015335:Molecular Probes; D010455:Peptides