title: Animal models using lymphocytic choriomeningitis virus.
abstract: This unit includes protocols for inducing systemic infection and persistent infection of mice with lymphocytic choriomeningitis virus (LCMV). Methods used to measure T cell responses to LCMV are then described. A protocol to assess anti-LCMV immunity in vivo is also included. Support protocols for preparing LCMV stocks and measuring LCMV titers using a plaque assay are also included. Finally, a support protocol for detecting anti-LCMV antibodies by ELISA is presented.
authors.affiliation: The Scripps Research Institute, La Jolla, California, USA.
authors.forename: M
authors.initials: M
authors.lastname: von Herrath
authors.forename: J L
authors.initials: JL
authors.lastname: Whitton
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004195:Disease Models, Animal; D008213:Lymphocyte Activation; D008216:Lymphocytic Choriomeningitis / Q000097:blood* / Q000821:virology*; D008217:Lymphocytic choriomeningitis virus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D010948:Viral Plaque Assay; D014776:Virus Cultivation
chemical_list: D000914:Antibodies, Viral