title: Preservation of non-heart-beating donor livers in extracorporeal liver perfusion and histidine-trytophan-ketoglutarate solution.
abstract: OBJECTIVE
  To compare the preservation of non-heart-beating donor (NHBD) livers in cold histidine-trytophan-ketoglutarate (HTK) solution and extracorporeal liver perfusion (ECLP).
  
  
  METHODS
  Livers harvested from healthy pigs were stored for 10 h in cold HTK solution (group A, n = 4) or perfused with oxygenated autologous blood at body temperature (group B, n = 4). Both groups were then tested on the circuit for 4 h. Bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of extracorporeal livers were tested in each group. Liver tissues from each group were examined at the end of reperfusion.
  
  
  RESULTS
  At 1, 2, 3 and 4 h after reperfusion, bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of livers in group A were statistically different from those in group B (P < 0.05 or P < 0.01).
  
  
  CONCLUSIONS
  ECLP is better than HTK solution to preserve NHBD livers. ECLP can assess the graft viability before liver transplantation.
authors.affiliation: Department of Surgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China. gongjin622@yahoo.com.cn
authors.forename: Jin
authors.initials: J
authors.lastname: Gong
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Lao
authors.forename: Xi-Mo
authors.initials: XM
authors.lastname: Wang
authors.forename: Gang
authors.initials: G
authors.lastname: Long
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D001646:Bile / Q000378:metabolism; D003080:Cold Temperature; D005112:Extracorporeal Circulation / Q000009:adverse effects; D005947:Glucose / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology; D006439:Hemodynamics / Q000187:drug effects; D008099:Liver / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000601:surgery* / Q000648:ultrastructure; D008102:Liver Circulation / Q000187:drug effects; D016031:Liver Transplantation; D008353:Mannitol / Q000009:adverse effects / Q000494:pharmacology; D009926:Organ Preservation / Q000009:adverse effects / Q000379:methods*; D019188:Organ Preservation Solutions / Q000009:adverse effects / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D011189:Potassium Chloride / Q000009:adverse effects / Q000494:pharmacology; D011343:Procaine / Q000009:adverse effects / Q000494:pharmacology; D015424:Reperfusion / Q000009:adverse effects; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control; D013552:Swine; D013997:Time Factors
chemical_list: C039984:Bretschneider cardioplegic solution; D019188:Organ Preservation Solutions; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose