Measurement of in vitro specific-antibody synthesis.
In this unit, a protocol is provided in which antigen-specific responses by human lymphocytes are elicited and then quantitated by the enzyme-linked immunosorbent assay (ELISA). Initially, Ficoll-Hypaque-isolated mononuclear cells are treated to remove cytophilic antibody. The antibody response being studied is then elicited by the appropriate stimulating antigen (in this case, influenza virus, although other antigens can also be used) or mitogen. Finally, after a 12-day incubation, the supernatant is harvested for assay.
Yarchoan
R
R
National Cancer Institute, Bethesda, Maryland, USA.
D000917:Antibody Formation / Q000276:immunology*; D000918:Antibody Specificity / Q000276:immunology*; D000941:Antigens / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D008214:Lymphocytes / Q000276:immunology*
D000941:Antigens