Gene delivery to the airway.
This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described. Methods are presented for generation of human bronchial xenografts as well as both ex vivo and in vivo gene transfer to these xenografts. Finally, a method for in vivo gene delivery to the lungs of rodents is included. Methods for evaluating transgene expression are given in the support protocols.
Duan
D
D
University of Iowa, Iowa City, Iowa, USA.
Zhang
Y
Y
Engelhardt
J F
JF
D000818:Animals; D001980:Bronchi / Q000378:metabolism / Q000637:transplantation; D004847:Epithelial Cells / Q000378:metabolism; D018014:Gene Transfer Techniques; D017930:Genes, Reporter; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors; D005826:Genetics, Medical; D006801:Humans; D051379:Mice; D008954:Models, Biological; D051381:Rats; D012137:Respiratory System / Q000378:metabolism*; D014183:Transplantation, Heterologous