Effect of low-level laser therapy in the inflammatory response induced by Bothrops jararacussu snake venom.
This article reports the effect of low-level laser therapy (LLLT) on the edema formation and leukocyte influx caused by Bothrops jararacussu snake venom as an alternative treatment for Bothrops snakebites. The inflammatory reaction was induced by injection of 0.6 mg/kg of B. jararacussu venom, in gastrocnemius muscle. Cell influx and edema were evaluated at 3 or 24h after venom injection. Mice were irradiated at the site of injury by a low-level laser (685 nm) with a dose of 4.2J/cm(2). A therapy that combines LLLT and antivenom was also studied. B. jararacussu venom caused a significant edema formation 3 and 24h after its injection, and a prominent leukocyte infiltrate composed predominantly of neutrophils at 24h after venom inoculation. LLLT significantly reduced edema formation by 53% and 64% at 3 and 24h, respectively, and resulted in a reduction of neutrophils accumulation (P<0.05). The combined therapy showed to be more efficient than each therapy acting separately. In conclusion, LLLT significantly reduced the edema and leukocyte influx into the envenomed muscle, suggesting that LLLT should be considered as a potentially therapeutic approach for the treatment of the local effects of Bothrops species.
Barbosa
Ana Maria
AM
Laboratory of Inflammation, Institute of Research and Development, University of Vale do Paraíba, Av. Shishima Hifumi, 2911, Urbanova, CEP 12244-000, São José dos Campos, SP, Brazil.
Villaverde
Antonio Balbin
AB
Guimarães-Souza
Ludmila
L
Ribeiro
Wellington
W
Cogo
José Carlos
JC
Zamuner
Stella Regina
SR
D000818:Animals; D000997:Antivenins / Q000494:pharmacology; D017837:Bothrops; D003131:Combined Modality Therapy; D003435:Crotalid Venoms / Q000008:administration & dosage / Q000009:adverse effects*; D004195:Disease Models, Animal; D004487:Edema / Q000139:chemically induced / Q000628:therapy; D007273:Injections, Intramuscular; D007962:Leukocytes / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects; D028022:Low-Level Light Therapy / Q000379:methods*; D008297:Male; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000473:pathology / Q000528:radiation effects*; D009220:Myositis / Q000139:chemically induced / Q000473:pathology / Q000628:therapy*
D000997:Antivenins; D003435:Crotalid Venoms