Cellulosic hydrogen production with a sequencing bacterial hydrolysis and dark fermentation strategy.
In this study, cellulose hydrolysis activity of two mixed bacterial consortia (NS and QS) was investigated. Combination of NS culture and BHM medium exhibited better hydrolytic activity under the optimal condition of 35 degrees C, initial pH 7.0, and 100rpm agitation. The NS culture could hydrolyze carboxymethyl cellulose (CMC), rice husk, bagasse and filter paper, among which CMC gave the best hydrolysis performance. The CMC hydrolysis efficiency increased with increasing CMC concentration from 5 to 50g/l. With a CMC concentration of 10g/l, the total reducing sugar (RS) production and the RS producing rate reached 5531.0mg/l and 92.9mg/l/h, respectively. Furthermore, seven H2-producing bacterial isolates (mainly Clostridium species) were used to convert the cellulose hydrolysate into H2 energy. With an initial RS concentration of 0.8g/l, the H2 production and yield was approximately 23.8ml/l and 1.21mmol H2/g RS (0.097mmol H2/g cellulose), respectively.
Lo
Yung-Chung
YC
Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.
Bai
Ming-Der
MD
Chen
Wen-Ming
WM
Chang
Jo-Shu
JS
D001419:Bacteria / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D002241:Carbohydrates; D002244:Carbon / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002482:Cellulose / Q000378:metabolism*; D003470:Culture Media; D005285:Fermentation / Q000187:drug effects; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis / Q000187:drug effects; D007700:Kinetics; D012722:Sewage; D013696:Temperature
D002241:Carbohydrates; D003470:Culture Media; D012722:Sewage; D002244:Carbon; D006859:Hydrogen; D002482:Cellulose