title: CE-MSn of complex pectin-derived oligomers.
abstract: As pectin molecules are too large and heterogeneous to analyze as a whole, the polymer is usually degraded to smaller oligomers, which are often analyzed by high-performance anion exchange chromatography (HPAEC). However, the high salt concentration necessary to elute pectin oligomers by HPAEC is incompatible with online mass detection. To overcome such a disadvantage, a CE-IT-MS system was set up to further elucidate the fine structure of charged oligosaccharides. An effective separation of differently substituted galacturonic acid containing oligomers was obtained by low-pH CE-LIF analysis. By adapting the buffer and capillary online MS detection was enabled. Moreover, with MS/MS it was possible to localize sugar residues' substitutions. With this combined CE-MS approach LIF electropherograms of xylogalacturonan and rhamnogalacturonan I digests could be annotated. The method was further exemplified by a complex oligomer mixture of acid hydrolyzed apple pectin, which was separated and characterized by CE-MSn. Oligomers present in low amounts could be localized by their corresponding m/z, as was demonstrated by selected mass range representation.
authors.affiliation: Laboratory of Food Chemistry, Department of Agrotechnology and Food Sciences, Wageningen University, Wageningen, The Netherlands.
authors.forename: Gerd-Jan
authors.initials: GJ
authors.lastname: Coenen
authors.forename: Mirjam A
authors.initials: MA
authors.lastname: Kabel
authors.forename: Henk A
authors.initials: HA
authors.lastname: Schols
authors.forename: Alphons G J
authors.initials: AG
authors.lastname: Voragen
mesh_terms: D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D027845:Malus / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D015394:Molecular Structure; D009844:Oligosaccharides / Q000032:analysis* / Q000737:chemistry; D010368:Pectins / Q000737:chemistry*; D011721:Pyrenes
chemical_list: C098210:1-aminopyrene-3,6,8-trisulfonic acid; D009844:Oligosaccharides; D011721:Pyrenes; D010368:Pectins