title: CD24 cross-linking induces apoptosis in, and inhibits migration of, MCF-7 breast cancer cells.
abstract: BACKGROUND
  The biological effects of CD24 (FL-80) cross-linking on breast cancer cells have not yet been established. We examined the impact of CD24 cross-linking on human breast cancer cell line MCF-7.
  
  
  METHODS
  MCF-7 and MDA-MB-231 cells were treated with anti-rabbit polyclonal IgG or anti-human CD24 rabbit polyclonal antibodies to induce cross-linking, and then growth was studied. Changes in cell characteristics such as cell cycle modulation, cell death, survival in three-dimensional cultures, adhesion, and migration ability were assayed after CD24 cross-linking in MCF-7.
  
  
  RESULTS
  Expression of CD24 was analyzed by flow cytometry in MDA-MB-231 and MCF-7 cells where 2% and 66% expression frequencies were observed, respectively. CD24 cross-linking resulted in time-dependent proliferation reduction in MCF-7 cells, but no reduction in MDA-MB-231 cells. MCF-7 cell survival was reduced by 15% in three-dimensional culture after CD24 cross-linking. Increased MCF-7 cell apoptosis was observed after CD24 cross-linking, but no cell cycle arrest was observed in that condition. The migration capacity of MCF-7 cells was diminished by 30% after CD24 cross-linking.
  
  
  CONCLUSIONS
  Our results showed that CD24 cross-linking induced apoptosis and inhibited migration in MCF-7 breast cancer cells. We conclude that CD24 may be considered as a novel therapeutic target for breast cancer.
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-744, Korea. kkimjp@hanmail.net
authors.forename: Jong Bin
authors.initials: JB
authors.lastname: Kim
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Ko
authors.forename: Wonshik
authors.initials: W
authors.lastname: Han
authors.forename: Jeong Eon
authors.initials: JE
authors.lastname: Lee
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Lee
authors.forename: Incheol
authors.initials: I
authors.lastname: Shin
authors.forename: Sangmin
authors.initials: S
authors.lastname: Kim
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Jihyoung
authors.initials: J
authors.lastname: Cho
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Bae
authors.forename: Hyeon-Gun
authors.initials: HG
authors.lastname: Jee
authors.forename: Dong-Young
authors.initials: DY
authors.lastname: Noh
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology*; D001943:Breast Neoplasms / Q000276:immunology / Q000473:pathology / Q000628:therapy; D051927:CD24 Antigen / Q000276:immunology*; D045744:Cell Line, Tumor; D003165:Complement System Proteins / Q000276:immunology* / Q000378:metabolism; D025461:Feedback, Physiological; D005260:Female; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000378:metabolism / Q000627:therapeutic use; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011817:Rabbits; D011961:Receptors, Fc / Q000378:metabolism; D015398:Signal Transduction
chemical_list: D051927:CD24 Antigen; D007141:Immunoglobulin Fc Fragments; D011961:Receptors, Fc; D003165:Complement System Proteins