[Luteolin excretion after oral administration of Elsholtzia blanda benth extracts in rats].
To observe the excretion of luteolin after oral administration of Elsholtzia blanda benth extracts in rats.



Samples of urine, feces and bile were collected after oral administration of Elsholtzia blanda benth extracts in rate. After deconjugation with beta-glucuronidase/sulfatase, the levels of luteolin in urine, feces and bile were measured by RP-HPLC.



The recovery rate of luteolin was 98.0 %-106.0 % and the extract recoveries were 85.0 %-108.0%. Relative standard deviation (RSD) of intra-and inter-day assay was less than 10.0 %. The total accumulative excretion was 37 % (11 % in urine, 26 % in feces and bile).



The established RP-HPLC method is sensitive, specific, accurate, and is applicable for determination of luteolin in rat urine,feces and bile.
Ying
Jing-yan
JY
Department of Pharmaceutical Analysis and Drug Metabolism, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Li
Xiang
X
Wang
Jin-lei
JL
Yao
Tong-wei
TW
D000284:Administration, Oral; D000818:Animals; D001646:Bile / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000378:metabolism / Q000493:pharmacokinetics; D005243:Feces / Q000737:chemistry; D019686:Lamiaceae / Q000737:chemistry*; D047311:Luteolin / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000652:urine; D008297:Male; D010936:Plant Extracts / Q000008:administration & dosage; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D047311:Luteolin