Characterizing recombinant proteins using HPLC gel filtration and mass spectrometry.
Recombinant proteins are subject to many forms of heterogeneity, including aggregation, proteolytic degradation, chemical modification, mutation, and incorrect translation. This unit describes methods for the detection and identification of these problems using analytical HPLC gel filtration and MALDI-MS. Preliminary characterization of recombinants is necessary before the structure or function of the protein can be investigated.
Begg
G E
GE
The Wistar Institute, Philadelphia, Pennsylvania, USA.
Harper
S L
SL
Speicher
D W
DW
D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D011994:Recombinant Proteins