title: Cellular incorporation into electrospun nanofibers: retained viability, proliferation, and function in fibroblasts.
abstract: Nanofibers are an emerging scaffold for tissue engineering. To date no one has reported cell incorporation into nanofibers. Human foreskin fibroblasts and human adipose-derived adult stem cells (hADAS) were grown to confluence, resuspended in phosphate-buffered saline, and then solubilized in polyvinyl alcohol (PVA). Nanofibers were created using an electrospinning technique across an electric potential of 20 kV. Cell interaction with nanofibers was assessed with optical microscopic imaging and scanning electron microscopy. PVA nanofibers with incorporated cells were then solubilized in phosphate-buffered saline; cell viability was assessed by trypan blue exclusion. Viable cells were allowed to proliferate. Chondrogenesis in fibroblasts was induced with TGF-beta1. Both fibroblasts and hADAS survived the electrospinning process and were incorporated into PVA nanofibers. hADAS cell proliferation was negligible; however, fibroblasts proliferated and showed retained ability to undergo chondrogenesis. Cells can be incorporated into nanofibers, with maintained viability, proliferation, and function.
authors.affiliation: Division of Plastic and Reconstructive Surgery, University of North Carolina, Chapel Hill, NC 27514, USA. john_vanaalst@med.unc.edu
authors.forename: John A
authors.initials: JA
authors.lastname: van Aalst
authors.forename: Courtney R
authors.initials: CR
authors.lastname: Reed
authors.forename: Li
authors.initials: L
authors.lastname: Han
authors.forename: Tony
authors.initials: T
authors.lastname: Andrady
authors.forename: Michael
authors.initials: M
authors.lastname: Hromadka
authors.forename: Susan
authors.initials: S
authors.lastname: Bernacki
authors.forename: Kamalkumar
authors.initials: K
authors.lastname: Kolappa
authors.forename: James B
authors.initials: JB
authors.lastname: Collins
authors.forename: Elizabeth G
authors.initials: EG
authors.lastname: Loboa
mesh_terms: D049109:Cell Proliferation; D002470:Cell Survival; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D049329:Nanostructures / Q000737:chemistry; D011108:Polymers; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D011108:Polymers