Determination of binding thermodynamics.
This unit serves as a starting point for exploring the thermodynamic properties of interactions between small molecules and DNA. It covers the determination of simple, apparent association/dissociation constants. The concentration of DNA-bound ligand and free ligand are determined and a binding constant is extracted from these data. Data gathering and curve fitting are discussed.
Dedon
P C
PC
Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002417:Cattle; D004247:DNA / Q000737:chemistry*; D003630:Daunorubicin / Q000737:chemistry; D004996:Ethidium / Q000737:chemistry; D008024:Ligands; D055095:Optics and Photonics; D013057:Spectrum Analysis; D013816:Thermodynamics
D008024:Ligands; D004247:DNA; C427203:calf thymus DNA; D004996:Ethidium; D003630:Daunorubicin