[The complex neurochemical assessment of brain proteins in mentally healthy subjects and schizophrenic patients].
Relative amounts of the glutamate metabolizing enzymes - glutamine synthetase, glutamine synthetase-like protein, three isoenzymes of glutamate dehydrogenase as well as creatine phosphokinase (a main astroglial energy metabolism enzyme) and major proteins of astro- and oligodendroglia - a glial fibrillary acidic protein and a myelin basic protein were determined in postmortem brain extracts from three areas - the prefrontal cortex, caudate nucleus and cerebellum - from mentally healthy subjects (n=21) and patients with chronic schizophrenia (n=23). To single out "metabolic types" the data obtained have been subjected to cluster analysis. It has been demonstrated for the first time that the cluster analysis of the biological parameters (enzymes and proteins) with correction for age, gender, postmortem interval and presence/absence of diagnosis, enables to distinguish "mentally healthy" cases and "schizophrenic patients" with a high degree of significance (mean mixing error <20%, small er, Cyrillic>>0,00004). Thus, we suppose that mentally healthy controls and patients with schizophrenia are objectively divided into different "metabolic types".
Burbaeva
G Sh
GSh
Boksha
I S
IS
Sudakov
S A
SA
Miasoedov
S N
SN
Savushkina
O K
OK
Tereshkina
E B
EB
Starodubtseva
L I
LI
Turishcheva
M S
MS
Vorob'eva
E A
EA
D000328:Adult; D000368:Aged; D019732:Amide Synthases / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D001921:Brain / Q000378:metabolism*; D016000:Cluster Analysis; D003402:Creatine Kinase / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005969:Glutamate Dehydrogenase / Q000378:metabolism*; D005974:Glutamate-Ammonia Ligase / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D012559:Schizophrenia / Q000378:metabolism*; D012720:Severity of Illness Index
D015415:Biomarkers; D009419:Nerve Tissue Proteins; D005969:Glutamate Dehydrogenase; D003402:Creatine Kinase; D019732:Amide Synthases; C417025:glutamine synthetase-like protein, human; D005974:Glutamate-Ammonia Ligase