Reversal effect of Ganoderma lucidum polysaccharide on multidrug resistance in K562/ADM cell line.
OBJECTIVE
To investigate the reversal effect of Ganoderma lucidum polysaccharides (Gl-PS) on multidrug resistance (MDR) in the adriamycin (ADM)-resistant leukemic cell line K562/ADM.


METHODS
Cytotoxicity was assayed by 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide method; the ADM concentration in cells was determined by flow cytometry and confocal laser scanning microscopy techniques; the expression of P-glycoprotein was assayed by flow cytometry; and the mRNA expression levels of MDR-1 and MDR-associated protein (MRP)1 were determined by RT-PCR.


RESULTS
Gl-PS reversed MDR in K562/ADM cells. Gl-PS obviously reversed the resistance of K562/ADM to doxorubicin. The reversing factors of Gl-PS at 10 and 20 mg/L were 6.46 and 6.80, respectively. MDR-1 and MRP1 transcription were downregulated by 10 and 50 mg/L Gl-PS.


CONCLUSIONS
Gl-PS can reverse the MDR by downregulating the expression of MDR-1 and MRP1 in K562/ADM cells.
Li
Wei-Dong
WD
Department of Pharmacology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100083, China. lwdpharma@126.com
Zhang
Bo-di
BD
Wei
Ran
R
Liu
Ji-Hong
JH
Lin
Zhi-Bin
ZB
D000903:Antibiotics, Antineoplastic / Q000378:metabolism*; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000378:metabolism*; D018432:Drug Resistance, Multiple / Q000187:drug effects* / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000187:drug effects; D005562:Formazans / Q000378:metabolism; D006801:Humans; D020014:K562 Cells; D011134:Polysaccharides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D020940:Reishi / Q000235:genetics / Q000378:metabolism*; D013778:Tetrazolium Salts / Q000378:metabolism
D000903:Antibiotics, Antineoplastic; D005562:Formazans; D011134:Polysaccharides; D012333:RNA, Messenger; D013778:Tetrazolium Salts; C079782:MTT formazan; D004317:Doxorubicin