Estimating the variation in S phase duration from flow cytometric histograms.
A stochastic model for interpreting BrdUrd DNA FCM-derived data is proposed. The model is based on branching processes and describes the progression of the DNA distribution of BrdUrd-labelled cells through the cell cycle. With the main focus on estimating the S phase duration and its variation, the DNA replication rate is modelled by a piecewise linear function, while assuming a gamma distribution for the S phase duration. Estimation of model parameters was carried out using maximum likelihood for data from two different cell lines. The results provided quite a good fit to the data, suggesting that stochastic models may be a valuable tool for analysing this kind of data.
Larsson
Sara
S
Centre for Mathematical Sciences, Division of Mathematical Statistics, Lund University, P.O. Box 118, 221 00 Lund, Sweden. sara@maths.lth.se
Ryden
Tobias
T
Holst
Ulla
U
Oredsson
Stina
S
Johansson
Maria
M
D001940:Breast / Q000166:cytology; D001943:Breast Neoplasms / Q000473:pathology; D002460:Cell Line; D004261:DNA Replication; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D008954:Models, Biological; D016196:S Phase / Q000502:physiology*; D013269:Stochastic Processes