title: Biological responses to chemokine superfamily members.
abstract: A detailed summary of all the chemokine subfamily members including alternative names, receptor-ligand pairs, receptor cellular distribution, and leukocyte responsiveness is presented here along with assays to measure the biological activities of chemokines on each of the major leukocyte subsets (i.e., microchemotaxis chambers, fluorescence-based assays, use of nitrocellulose filters and membranes coated with extracellular matrix proteins, cellular adhesion assays, mobilization of intracellular free calcium, actin polymerization, degranulation assays, and the use of chemokine receptor-specific antibodies in flow cytometric analysis and radiolabeled chemokine binding assays).
authors.affiliation: National Institute on Aging, NIH, Washington, D.C.
authors.forename: Dennis D
authors.initials: DD
authors.lastname: Taub
authors.affiliation: National Institute on Aging, NIH, Washington, D.C.
authors.forename: Eric
authors.initials: E
authors.lastname: Schaffer
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002118:Calcium / Q000097:blood; D016764:Cell Polarity; D018925:Chemokines / Q000032:analysis / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte; D006801:Humans; D007962:Leukocytes / Q000166:cytology / Q000502:physiology*; D016131:Lymphocyte Subsets / Q000166:cytology / Q000502:physiology*; D019707:Receptors, Chemokine / Q000276:immunology / Q000378:metabolism
chemical_list: D000199:Actins; D018925:Chemokines; D019707:Receptors, Chemokine; D002118:Calcium