Immunophenotyping of T cell subpopulations in HIV disease.
This unit illustrates the challenges associated with measurements of T cell subpopulations, and describes how those challenges can be overcome by using polychromatic (5+ color) approaches to flow cytometry. Such approaches are immensely powerful and directly applicable to studies of T cell biology in HIV disease; however, their development requires careful consideration of the antibodies and fluorochromes employed, as discussed here. Furthermore, factors generally known to influence T cell counts can also govern the success or failure of polychromatic experiments; therefore, these factors are reviewed and provide recommendations for minimizing their influence on polychromatic measurements of T cell subsets.
Chattopadhyay
Pratip K
PK
National Institutes of Health, Bethesda, Maryland, USA.
Roederer
Mario
M
D002454:Cell Differentiation; D015658:HIV Infections / Q000276:immunology*; D006801:Humans; D007156:Immunologic Memory; D016130:Immunophenotyping / Q000379:methods*; D008213:Lymphocyte Activation; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology