[An experimental study on rabbit's radial bone defect healed by application of mimetic periosteum with tissue-engineered bone].
OBJECTIVE
The purpose of this study is to investigate the osteogenic potential and possibility of combination application of mimetic osteoinductive periosteum with tissue-engineered bone.


METHODS
The three-dimensional construction of tissue-engineered bone was made by implantation of adipose derived stromal cells (ADSCs) into rhBMP-2 mediated bio-derived carrier, and mimetic periosteum was constructed by loading ADSCs into Cs-Col-beta3-TCP with rhBMP-2. 10 mm defects of right radiuses were established in adult New Zealand rabbits, group A was transplanted by tissue-engineered bone with mimetic periosteum, group B was implanted by tissue-engineered bone, and group C was implanted by mimetic periosteum, group D was transplanted by bio-derived compound bone as blank scaffold. X-ray, histology, immunohistochemistry stain, dural energy X-ray absorptiometry (DEXA) and transmission electron microscopy (TEM) examinations were performed at different periods.


RESULTS
Group A played a predominant role in process of new tissue regeneration and mature bone reconstitution, defect completely healed at 12 weeks. Group B showed primary repair, group C also existed in modeling stage. While, group D displayed retard regeneration with poor osteogenic capacity. DEXA result showed that group A had statistical significance over control group according to data of BMC and BMD ( P < 0.05).


CONCLUSIONS
Enhanced osteogenic potential can be obtained by using tissue-engineered bone with mimetic osteoinductive periosteum. Defect can be healed with concord pattern of osteoinductive and osteopromotive and osteoconductive effects.
Guo
Hong-Gang
HG
Department of Orthopaedic Surgery , Tianjin Medical University General Hospital, Tianjin 300052, China.
Yao
Fang-Lian
FL
Ma
Xin-Long
XL
Yao
Kang-De
KD
D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration; D018786:Bone Substitutes; D008297:Male; D010521:Periosteum; D011817:Rabbits; D011884:Radius / Q000473:pathology / Q000601:surgery*; D023822:Tissue Engineering / Q000379:methods
D001672:Biocompatible Materials; D018786:Bone Substitutes