Measurement of soluble and membrane-bound interleukin 1 using a fibroblast bioassay.
Interleukin 1 (IL-1) is both a major mediator of inflammation and an important signal for activation and differentiation of lymphoid cells. IL-1 is produced by a large variety of cell types, but in most immunologic systems, activated macrophages are its major cellular source. This unit describes a bioassay for testing biological fluids based on the ability of IL-1 to induce IL-8 or IL-6 in fibroblasts. Also listed are sources of commercially available kits (ELISAs, immunoassays) to measure IL-1, and antibodies useful in setting up these assays.
Dinarello
C A
CA
University of Colorado Health Sciences Center, Denver, Colorado, USA.
Muegge
K
K
Durum
S K
SK
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001826:Body Fluids / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D007375:Interleukin-1 / Q000032:analysis* / Q000378:metabolism; D015850:Interleukin-6 / Q000096:biosynthesis; D016209:Interleukin-8 / Q000096:biosynthesis; D051379:Mice; D051381:Rats
D007375:Interleukin-1; D015850:Interleukin-6; D016209:Interleukin-8