Mesenchymal stem cells enhance bone regeneration in rat calvarial critical size defects more than platelete-rich plasma.
OBJECTIVE
The objective of this study was to compare culture-expanded bone marrow-derived mesenchymal stem cell and PRP loaded to natural bone mineral (Bio-Oss) and beta-TCP for rat calvarial bone repair.


METHODS
Twenty-two adult male Sprague-Dawley rats were randomly divided into 2 groups (n = 11). In the first group, 2 calvarial defects, 5 mm in diameter, were prepared in the parietal bone. The left defect was filled with Bio-Oss plus PRP while the right defect was filled with mesenchymal stem cell cultured on Bio-Oss. Kasios as a bone substitute was replaced in the second group. The animals were humanely killed 6 weeks postsurgery and the amount of the bone regeneration evaluated using histometric analysis.


RESULTS
The bone fill length in the calvarial defect had statistically significant difference with other groups (P < .05). In both groups, mesenchymal stem cell revealed a higher percentage of bone formation in comparison with the PRP groups.


CONCLUSIONS
New synthetic bone substitutes may offer a better condition for bone regeneration compared to the traditional bone substitute in combination with mesenchymal stem cells. They remained in the defect and contributed bone regeneration. The use of the mesenchymal stem cell in conjunction with bone substitutes can enhance bone regeneration more than PRP.
Khojasteh
Arash
A
Oral and Maxillofacial Surgery, Taleghani University Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Eslaminejad
Mohamadreza Baghaban
MB
Nazarian
Hamid
H
D000818:Animals; D001861:Bone Regeneration; D018786:Bone Substitutes; D002130:Calcium Phosphates; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D008903:Minerals; D015676:Osteonectin / Q000032:analysis; D053495:Osteopontin / Q000032:analysis; D010294:Parietal Bone / Q000601:surgery; D053657:Platelet-Rich Plasma; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds
C077540:Bio-Oss; D018786:Bone Substitutes; D002130:Calcium Phosphates; D008903:Minerals; D015676:Osteonectin; C485817:beta-tricalcium phosphate; D053495:Osteopontin