title: The polymerase chain reaction.
abstract: This appendix describes the methodology behind PCR and gives a method for amplifying DNA enzymatically by the PCR and for optimizing this reaction for the sequence and primer set of interest.
authors.affiliation: Harvard Medical School, Boston, Massachusetts.
authors.forename: Martha F
authors.initials: MF
authors.lastname: Kramer
authors.affiliation: Harvard Medical School, Boston, Massachusetts.
authors.forename: Donald M
authors.initials: DM
authors.lastname: Coen
mesh_terms: D000818:Animals; D004247:DNA / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D019914:Taq Polymerase / Q000378:metabolism
chemical_list: D004247:DNA; D019914:Taq Polymerase