title: IgG isotype profile is correlated with cardiomegaly in Beagle dogs infected with distinct Trypanosoma cruzi strains.
abstract: A systematic study following infection by various strains of the protozoan parasite, Trypanosoma cruzi, and the simultaneous monitoring of the humoral immune response together with the elicited cellular response, could add greatly to our understanding of differences between strains of this important human pathogen. In that sense, acute and chronic infections with distinct T. cruzi strains (Y, Berenice-78 and ABC) in Beagle dogs were studied through a longitudinal evaluation of immunoglobulin G (IgG), IgG1 and IgG2 isotypes (by ELISA and flow cytometry (FC)), as well as measurements of peripheral blood mononuclear cell (PBMC) proliferation over a 100-week period, and their correlation with cardiomegaly. Our results show that infected animals presenting cardiomegaly showed lower or absent levels of IgG1 during the chronic phase of the infection, when compared to those that did not show an increase in heart weight. In that manner, our results suggest that IgG1 could be used as a marker for cardiac pathogenicity in Chagas disease.
authors.affiliation: Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. guedespm@nupeb.ufop.br
authors.forename: Paulo M M
authors.initials: PM
authors.lastname: Guedes
authors.forename: Vanja M
authors.initials: VM
authors.lastname: Veloso
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Gollob
authors.forename: Luis C C
authors.initials: LC
authors.lastname: Afonso
authors.forename: Ivo S
authors.initials: IS
authors.lastname: Caldas
authors.forename: Priscila
authors.initials: P
authors.lastname: Vianna
authors.forename: Marta
authors.initials: M
authors.lastname: de Lana
authors.forename: Egler
authors.initials: E
authors.lastname: Chiari
authors.forename: Maria T
authors.initials: MT
authors.lastname: Bahia
authors.forename: Lúcia M C
authors.initials: LM
authors.lastname: Galvão
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D006332:Cardiomegaly / Q000276:immunology* / Q000469:parasitology*; D048708:Cell Growth Processes / Q000276:immunology; D014355:Chagas Disease / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D004283:Dog Diseases / Q000276:immunology* / Q000469:parasitology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005434:Flow Cytometry / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007700:Kinetics; D007963:Leukocytes, Mononuclear / Q000276:immunology; D009929:Organ Size; D012044:Regression Analysis; D014349:Trypanosoma cruzi / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D007074:Immunoglobulin G