Polyacrylamide gel electrophoresis (PAGE) of synthetic nucleic acids.
Protocols are given for analysis of oligonucleotides by PAGE, using either methylene blue staining or radiolabeling to mark the oligonucleotide. In addition, a separate protocol is provided for purification by PAGE.
Andrus
A
A
PE Applied Biosystems, Foster City, California, USA.
Kuimelis
R G
RG
D020106:Acrylamide; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D008751:Methylene Blue; D009696:Nucleic Acids / Q000032:analysis* / Q000138:chemical synthesis* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000378:metabolism; D011116:Polynucleotide 5'-Hydroxyl-Kinase / Q000378:metabolism; D011868:Radioisotopes; D013194:Staining and Labeling; D014466:Ultraviolet Rays
D009696:Nucleic Acids; D009841:Oligonucleotides; D011868:Radioisotopes; D020106:Acrylamide; D011116:Polynucleotide 5'-Hydroxyl-Kinase; D008751:Methylene Blue