title: Cellular compartment analysis of temporal activity by fluorescence in situ hybridization (catFISH).
abstract: This protocol describes a method (cellular compartment analysis of temporal activity by fluorescent in situ hybridization or catFISH) that uses fluorescent in situ hybridization to immediate-early gene RNAs and confocal microscopy to identify neuronal populations activated at two distinct times. The combination of cellular and temporal resolution makes catFISH a valuable tool for investigating the dynamic interactions of neuronal populations associated with different behaviors or cognitive challenges.
authors.affiliation: University of Arizona, Tucson, Arizona, USA.
authors.forename: J F
authors.initials: JF
authors.lastname: Guzowski
authors.forename: P F
authors.initials: PF
authors.lastname: Worley
mesh_terms: D022082:Cellular Structures / Q000737:chemistry* / Q000502:physiology*; D015342:DNA Probes / Q000032:analysis / Q000502:physiology; D017781:Genes, Immediate-Early / Q000502:physiology; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012313:RNA / Q000032:analysis / Q000502:physiology; D013997:Time Factors
chemical_list: D015342:DNA Probes; D012313:RNA