title: Kinetic modeling of enzymatic hydrolysis of cellulose in differently pretreated fibers from dairy manure.
abstract: A kinetic model incorporating dynamic adsorption, enzymatic hydrolysis, and product inhibition was developed for enzymatic hydrolysis of differently pretreated fibers from a nitrogen-rich lignocellulosic material-dairy manure. The effects of manure proteins on the enzyme adsorption profile during hydrolysis have been discussed. Enzyme activity, instead of protein concentration, was used to describe the enzymatic hydrolysis in order to avoid the effect of manure protein on enzyme protein analysis. Dynamic enzyme adsorption was modeled based on a Langmiur-type isotherm. A first-order reaction was applied to model the hydrolysis with consideration being given for the product inhibition. The model satisfactorily predicted the behaviors of enzyme adsorption, hydrolysis, and product inhibition for all five sample manure fibers. The reaction conditions were the substrate concentrations of 10-50 g/L, enzyme loadings of 7-150 FPU/g total substrate, and the reaction temperature of 50 degrees C.
authors.affiliation: Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, Michigan, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Liao
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Wen
authors.forename: Craig
authors.initials: C
authors.lastname: Frear
authors.forename: Shulin
authors.initials: S
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D044602:Cellulases / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006868:Hydrolysis; D007700:Kinetics; D008372:Manure; D013696:Temperature
chemical_list: D004791:Enzyme Inhibitors; D008372:Manure; D002482:Cellulose; D044602:Cellulases