Quantum dot-based protein micro- and nanoarrays for detection of prostate cancer biomarkers.
In this article, we demonstrate the fabrication and detection of cancer protein biochips consisting of micro- and nanoarrays whereby pegylated quantum dots (QDs) conjugated to antibodies (Abs) of prostate specific antigens (PSA) were used for the detection of clinical biomarkers such as PSA. BSA which acts as an efficient blocking layer in microarrays, tends to show an interaction with QDs. In view of this fact, we investigated two series of samples which were fabricated in the presence and absence of BSA blocking layer. Variation in the incubation time required for the antigen-antibody interaction to take place, different proteins as controls and the effect of bare QDs on these microarrays, were the three main parameters which were studied in these two series. Samples fabricated in the absence of BSA blocking layer exhibited an extremely high specificity in the detection of cancer proteins and were also marked by negligible nonspecific binding effects of QDs, in stark contrast to the samples fabricated using BSA as a blocking layer. Fabrication of nanoarrays of QD-conjugated PSA Abs having a spot size of nearly 900 nm has also been demonstrated. Thus, we show the potential offered by QDs in in vitro analysis of cancer biomarker imaging.
Gokarna
Anisha
A
Nano-Bio-Photonics Laboratory, Department of Physics, Chungbuk National University, Cheongju, Korea.
Jin
Li-Hua
LH
Hwang
Jun Seok
JS
Cho
Yong-Hoon
YH
Lim
Yong Taik
YT
Chung
Bong Hyun
BH
Youn
Seong Hun
SH
Choi
Dong Sik
DS
Lim
Jung Hyurk
JH
D000818:Animals; D014408:Biomarkers, Tumor; D002417:Cattle; D006801:Humans; D008297:Male; D036103:Nanotechnology / Q000379:methods*; D009363:Neoplasm Proteins / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D017430:Prostate-Specific Antigen / Q000737:chemistry; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000378:metabolism*; D040081:Protein Array Analysis; D025941:Protein Interaction Mapping / Q000379:methods; D045663:Quantum Dots; D012710:Serum Albumin, Bovine / Q000737:chemistry
D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D012710:Serum Albumin, Bovine; D017430:Prostate-Specific Antigen