Gene delivery to muscle.
The delivery of genes to skeletal muscle by myoblast implantation, DNA injection, or viral transduction has therapeutic applications for human neuromuscular and systemic disorders, many of which are now represented by transgenic or "knockout" mouse models. This unit describes the isolation and retroviral transduction of mouse myoblasts, the injection of myoblasts and plasmid DNA into mouse muscle, and histological methods for analyzing the recipient muscle. A procedure describing the injection of plasmid DNA into muscle with or without electric charge is also included.
Springer
Matthew L
ML
Stanford University School of Medicine, Stanford, California, USA.
Rando
Thomas A
TA
Blau
Helen M
HM
D000818:Animals; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D005826:Genetics, Medical; D006801:Humans; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism*; D032448:Myoblasts, Skeletal / Q000378:metabolism / Q000637:transplantation; D010957:Plasmids / Q000235:genetics; D012190:Retroviridae / Q000235:genetics; D014161:Transduction, Genetic