title: Protein lysine methyltransferase G9a acts on non-histone targets.
abstract: By methylation of peptide arrays, we determined the specificity profile of the protein methyltransferase G9a. We show that it mostly recognizes an Arg-Lys sequence and that its activity is inhibited by methylation of the arginine residue. Using the specificity profile, we identified new non-histone protein targets of G9a, including CDYL1, WIZ, ACINUS and G9a (automethylation), as well as peptides derived from CSB. We demonstrate potential downstream signaling pathways for methylation of non-histone proteins.
authors.affiliation: Biochemistry Laboratory, School of Engineering and Science, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Rathert
authors.forename: Arunkumar
authors.initials: A
authors.lastname: Dhayalan
authors.forename: Marie
authors.initials: M
authors.lastname: Murakami
authors.forename: Xing
authors.initials: X
authors.lastname: Zhang
authors.forename: Raluca
authors.initials: R
authors.lastname: Tamas
authors.forename: Renata
authors.initials: R
authors.lastname: Jurkowska
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Komatsu
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Shinkai
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Cheng
authors.forename: Albert
authors.initials: A
authors.lastname: Jeltsch
mesh_terms: D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000737:chemistry* / Q000378:metabolism; D008745:Methylation; D040081:Protein Array Analysis; D011496:Protein Methyltransferases; D015398:Signal Transduction; D013379:Substrate Specificity
chemical_list: D000076983:Histone Methyltransferases; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase