title: A simple method of infecting rabbits with Bovine herpesvirus 1 and 5.
abstract: This report describes an alternative technique to inoculate rabbits and to reproduce infection by Bovine herpesvirus 1 and 5. First, the nostrils are anaesthetized by aspersion with local anaesthetic. A few seconds later, and after proving the insensitivity of the zone, the rabbits are put on their back legs with their nostrils upwards and the inoculum is introduced slowly into each nostril by using disposable droppers. Clinical signs, viral isolation from nasal swabs, histological lesions found, positive polymerase chain reaction and antibodies production confirm the infection. This very simple and bloodless technique, where the animals are exposed to minor distress, may be useful for evaluating the virulence of BoHV-1 and BoHV-5 strains, to study the establishment of latent virus infection and to test the potential of experimental vaccines or properties of antiviral drugs. It may be also suitable for experimental infection with other respiratory viruses in this animal model.
authors.affiliation: Department of Virology, Faculty of Veterinary Sciences, National University of La Plata, 60 and 118, La Plata, CP 1900, Buenos Aires, Argentina.
authors.forename: A R
authors.initials: AR
authors.lastname: Valera
authors.forename: C L
authors.initials: CL
authors.lastname: Pidone
authors.forename: A R
authors.initials: AR
authors.lastname: Massone
authors.forename: M A
authors.initials: MA
authors.lastname: Quiroga
authors.forename: J G
authors.initials: JG
authors.lastname: Riganti
authors.forename: S G
authors.initials: SG
authors.lastname: Corva
authors.forename: C M
authors.initials: CM
authors.lastname: Galosi
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004195:Disease Models, Animal; D018792:Encephalitis, Viral / Q000503:physiopathology / Q000821:virology*; D005260:Female; D006566:Herpesviridae Infections / Q000503:physiopathology / Q000821:virology*; D007242:Herpesvirus 1, Bovine / Q000254:growth & development* / Q000302:isolation & purification; D025881:Herpesvirus 5, Bovine / Q000254:growth & development* / Q000302:isolation & purification; D008099:Liver / Q000473:pathology; D008168:Lung / Q000473:pathology; D008297:Male; D008590:Meningoencephalitis / Q000503:physiopathology / Q000821:virology*; D009666:Nose / Q000821:virology; D011817:Rabbits; D014773:Virology / Q000379:methods*
chemical_list: D000914:Antibodies, Viral