Transfection using DEAE-dextran.
Two protocols for DEAE-dextran transfection of cells are provided in this unit. The Basic Protocol describes a procedure used to transfect adherent cells and the first Alternate Protocol presents a method used to transfect suspension cells. If an increase in transfection efficiency is needed, cells can be treated with chloroquine as described in the second Alternate Protocol.
Selden
Richard F
RF
Transkaryotic Therapies, Cambridge, Massachusetts.
D000818:Animals; D018501:Antirheumatic Agents / Q000737:chemistry / Q000494:pharmacology; D002478:Cells, Cultured; D002738:Chloroquine / Q000737:chemistry / Q000494:pharmacology; D003637:DEAE-Dextran / Q000737:chemistry*; D006801:Humans; D014162:Transfection / Q000379:methods*
D018501:Antirheumatic Agents; D002738:Chloroquine; D003637:DEAE-Dextran