title: Evaluation of 8-hydroxydeoxyguanosine, thiobarbituric acid-reactive substances and total antioxidant status as possible disease markers in oesophageal malignancies.
abstract: OBJECTIVE
  Evaluation of oxidative stress and diagnostic utility of its markers in oesophageal squamous cell carcinoma (OSCC).
  
  
  METHODS
  Serum 8-hydroxydeoxyguanosine, thiobarbituric acid-reactive substances (TBARS) and total antioxidant status (TAS) were measured in OSCC (n=75), non-malignant oesophageal diseases (n=30), and healthy subjects (n=79). Three months following oesophagectomy the measurements were repeated.
  
  
  RESULTS
  Exclusively in OSCC, 8-hydroxydeoxyguanosine and TBARS were elevated. TAS was reduced in non-malignancies compared to controls, and in OSCC compared to non-malignancies and controls. Only 8-hydroxydeoxyguanosine was associated with disease progression, lymph node involvement in particular. All indices were good indicators of cancer presence (ROC analysis) and normalized following oesophagectomy. A positive linear relationship between 8-hydroxydeoxyguanosine and TBARS, and negative non-linear between TAS and both 8-hydroxydeoxyguanosine and TBARS was demonstrated.
  
  
  CONCLUSIONS
  OSCC is associated with oxidative stress, attenuated following oesophagectomy. Consumption of serum antioxidants prevents accumulation of oxidatively modified molecules in non-malignancies. High accuracy of oxidative stress markers in indicating cancer presence warrants further investigation on their possible application as discriminatory markers and in monitoring treatment efficacy.
authors.affiliation: Department of Gastrointestinal and General Surgery, Wroclaw Medical University, Wroclaw, Poland. ddiakow@chir.am.wroc.pl
authors.forename: Dorota
authors.initials: D
authors.lastname: Diakowska
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Krzystek-Korpacka
authors.forename: Andrzej
authors.initials: A
authors.lastname: Lewandowski
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Grabowski
authors.forename: Witold
authors.initials: W
authors.lastname: Diakowski
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000000981:diagnostic imaging / Q000473:pathology; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D003937:Diagnosis, Differential; D004935:Esophageal Diseases / Q000097:blood*; D004938:Esophageal Neoplasms / Q000097:blood* / Q000000981:diagnostic imaging / Q000473:pathology; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology; D009367:Neoplasm Staging; D018384:Oxidative Stress / Q000502:physiology; D011859:Radiography; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D014408:Biomarkers, Tumor; D017392:Thiobarbituric Acid Reactive Substances; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine