Stepwise solid-phase synthesis of nucleopeptides.
Phosphodiester-linked peptide-oligonucleotide conjugates (nucleopeptides) are obtained by stepwise solid-phase procedures. The peptide is first assembled on a suitably derivatized solid matrix and the oligonucleotide is subsequently elongated at the free hydroxyl group of the linking amino acid. Temporary acid-labile and permanent base-labile protecting groups are combined. Careful choice of the protection scheme is required to prevent and minimize side reactions that may degrade the target molecule.
Grandas
Anna
A
Institut de Biomedicina de la Universitat de Barcelona, Barcelona, Spain.
Marchán
Vicente
V
Debéthune
Laurent
L
Pedroso
Enrique
E
D000596:Amino Acids / Q000737:chemistry; D009687:Nuclear Proteins / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry
D000596:Amino Acids; D009687:Nuclear Proteins; D010455:Peptides