[Enrichment process of gentiopicroside from the leaves of Gentiana macropylla with macroporous resin].
OBJECTIVE
To select the best type of macroporous resin for enriching gentiopicroside from the leaves of Gentiana macropylla, and study its optimal conditions and parameters.


METHODS
Adsorption and desorption characteristics of gentiopieroside were carried out on the resins. HPLC was used to determine the content of gentiopieroside.


RESULTS
The better resin D-101 was chosen, and the optimal parameters were obtained.


CONCLUSIONS
The process is feasible to enrich gentiopicroside from the leaves of Gentiana macropylla Pall.
Zhao
Yong
Y
Hunan Province Institute for Drug Control, Changsha 410001, China. fengni2002@sohu.com
Zhang
Ru
R
Sun
Wen-ji
WJ
D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D029965:Gentianaceae / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057888:Iridoid Glucosides; D039823:Iridoids / Q000737:chemistry / Q000302:isolation & purification*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D012117:Resins, Synthetic; D013678:Technology, Pharmaceutical / Q000379:methods*
D004365:Drugs, Chinese Herbal; D005960:Glucosides; D057888:Iridoid Glucosides; D039823:Iridoids; D012117:Resins, Synthetic; C012997:gentiopicroside; D000431:Ethanol