title: Genotyping by ligation assays.
abstract: This unit describes two methods of genotyping DNA sequences containing known nucleotide variations. The first protocol describes a colorimetric method for genotyping DNA samples amplified by the polymerase chain reaction (PCR) using an oligonucleotide ligation assay (OLA). The second protocol describes the ligase chain reaction (LCR), a method for simultaneously amplifying and genotyping genomic DNA samples. A Support Protocol describes the preparation of modified biotin- and digoxigenin-labeled oligonucleotide primers.
authors.affiliation: University of Washington School of Medicine, Seattle, Washington, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Nickerson
authors.forename: W
authors.initials: W
authors.lastname: Ankener
authors.forename: C
authors.initials: C
authors.lastname: Delahunty
authors.forename: P Y
authors.initials: PY
authors.lastname: Kwok
mesh_terms: D003124:Colorimetry; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D005826:Genetics, Medical; D005838:Genotype; D006801:Humans; D022521:Ligase Chain Reaction / Q000379:methods*; D016133:Polymerase Chain Reaction
chemical_list: D017931:DNA Primers; D004247:DNA