In vitro and in vivo anti-hepatitis B virus activities of a plant extract from Geranium carolinianum L.
Natural products provide a large reservoir of potentially active agents with anti-hepatitis B virus (HBV) activity. We examined the effect of the polyphenolic extract from Geranium carolinianum L. (PPGC) on HBV replication both in vitro and in vivo. In the human HBV-transfected liver cell line HepG(2) 2.2.15, PPGC effectively suppressed the secretion of the HBV antigens in a dose-dependent manner with IC(50) values of 46.85 microg/ml for HBsAg and 65.60 microg/ml for HBeAg at day 9. Consistent with the HBV antigen reduction, PPGC (100 microg/ml) also reduced HBV DNA level by 35.9%. In the duck hepatitis B virus (DHBV) infected ducks, after PPGC was dosed intragastricly (i.g.) once a day for 10 days, the plasma DHBV DNA level was reduced, with an ED(50) value of 47.54 mg/kg. In addition, Southern blot analysis confirmed the in vivo anti-HBV effect of PPGC in ducks and PPGC also reduced the plasma and the liver DHBV DNA level in a dose-dependent manner. Furthermore, significant improvement of the liver was observed after PPGC treatment, as evaluated by the histopathological analysis.
Li
Jiyang
J
Department of Biosynthetic Medicinal Chemistry, School of Pharmaceutical Sciences, Fudan University, Shanghai 200032, PR China.
Huang
Hai
H
Feng
Meiqing
M
Zhou
Wei
W
Shi
Xunlong
X
Zhou
Pei
P
D000818:Animals; D000956:Antigens, Viral / Q000096:biosynthesis; D000998:Antiviral Agents / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D002460:Cell Line; D004279:DNA, Viral / Q000096:biosynthesis / Q000097:blood; D004305:Dose-Response Relationship, Drug; D004372:Ducks; D031315:Geranium / Q000737:chemistry*; D018347:Hepadnaviridae Infections / Q000188:drug therapy; D015611:Hepatitis B Virus, Duck / Q000187:drug effects; D006515:Hepatitis B virus / Q000187:drug effects*; D006524:Hepatitis, Viral, Animal / Q000188:drug therapy; D022781:Hepatocytes / Q000821:virology; D006801:Humans; D020128:Inhibitory Concentration 50; D008099:Liver / Q000473:pathology / Q000821:virology; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D019562:Viral Load; D014779:Virus Replication
D000956:Antigens, Viral; D000998:Antiviral Agents; D004279:DNA, Viral; D010936:Plant Extracts