title: Synthesis and characterization of chimeric 2-5A-DNA oligonucleotides.
abstract: This unit provides protocols for the synthesis and characterization of 2-5A-antisense nucleic acids. These chimeric oligonucleotides consist of 2',5'-phosphodiester-linked oligoadenylates ligated to 3',5'-deoxyribonucleotides and are readily prepared using phosphoramidite chemistry on CPG solid supports. The 3',5'-deoxyribonucleotide functions as the antisense domain to target a given mRNA sequence, while the 2',5'-phosphodiester-linked oligoadenylate serves to locally activate 2-5A-dependent RNase L, causing the targeted sequence to be cleaved.
authors.affiliation: Northern Arizona University, Flagstaff, Arizona, USA.
authors.forename: M R
authors.initials: MR
authors.lastname: Player
authors.forename: P F
authors.initials: PF
authors.lastname: Torrence
mesh_terms: D000227:Adenine Nucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000138:chemical synthesis* / Q000737:chemistry*; D016376:Oligonucleotides, Antisense / Q000138:chemical synthesis* / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000737:chemistry; D010727:Phosphoric Diester Hydrolases / Q000378:metabolism; D017422:Sequence Analysis, DNA; D012910:Snake Venoms
chemical_list: D000227:Adenine Nucleotides; D016376:Oligonucleotides, Antisense; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D012910:Snake Venoms; C434331:phosphoramidite; C023505:2',5'-oligoadenylate; D004247:DNA; D010727:Phosphoric Diester Hydrolases