title: Male genital tract compartmentalization of human immunodeficiency virus type 1 (HIV).
abstract: We present phylogenetic evidence supporting viral compartmentalization between the blood (peripheral blood mononuclear cells or plasma) and multiple genitourinary sites in HIV-infected men. Four of the five subjects evaluated demonstrated compartmentalization of viral sequences between urogenital tract specimens (tissue or fluid) and at least one blood category. HIV sequence migration from blood to urogenital tract was detected in four of five men, with migration from urogenital tract to blood in the fifth, and cross migration between both compartments noted in one man. These observations add 5 additional cases to the 27 total reported cases in which male urogenital tract compartmentalization has been studied, investigate surgical samples/specimens that have not been evaluated previously, and provide further evidence for restricted flow of HIV between the blood and the genital tract. As such, our study findings are important for understanding the long-term response to antiretroviral therapy, the design of vaccines, and the sexual transmission of HIV.
authors.affiliation: Department of Laboratory Medicine, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Kurt
authors.initials: K
authors.lastname: Diem
authors.forename: David C
authors.initials: DC
authors.lastname: Nickle
authors.forename: Alexis
authors.initials: A
authors.lastname: Motoshige
authors.forename: Alan
authors.initials: A
authors.lastname: Fox
authors.forename: Susan
authors.initials: S
authors.lastname: Ross
authors.forename: James I
authors.initials: JI
authors.lastname: Mullins
authors.forename: Lawrence
authors.initials: L
authors.lastname: Corey
authors.forename: Robert W
authors.initials: RW
authors.lastname: Coombs
authors.forename: John N
authors.initials: JN
authors.lastname: Krieger
mesh_terms: D018562:Disease Transmission, Infectious; D005837:Genitalia, Male / Q000821:virology*; D015658:HIV Infections / Q000635:transmission / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D008969:Molecular Sequence Data; D009068:Movement; D010802:Phylogeny; D011533:Proviruses / Q000145:classification / Q000235:genetics; D017421:Sequence Analysis; D019562:Viral Load; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics
chemical_list: D054299:env Gene Products, Human Immunodeficiency Virus