[Agrobacterium tumefaciens-mediated transformation of the pathogen of Botrytis cinerea].
Employing gfp as a reporter gene and hygromycin gene (hph) as a selection marker, the recombinant vector pKPG was constructed and transformed into fresh conidia of Botrytis cinerea via Agrobacterium tumefaciens. Transformants were identified by PCR analysis of gfp and hph cassette, green fluorescence observation with microscope and Southern hybridization. Results confirmed that target genes were successfully integrated into the genome of Botrytis cinerea.
Shen
Wei-Feng
WF
Sericultural Research Institute, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China. weifsh@126.com
Weng
Hong-Biao
HB
Niu
Bao-Long
BL
He
Li-Hua
LH
Liu
Yan
Y
Qi
Xiao-Peng
XP
Meng
Zhi-Qi
ZQ
D016960:Agrobacterium tumefaciens / Q000235:genetics*; D015139:Blotting, Southern; D020171:Botrytis / Q000235:genetics; D002934:Cinnamates / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D014170:Transformation, Genetic / Q000235:genetics*
D002934:Cinnamates; D006921:Hygromycin B; C026273:hygromycin A