Influence of pH upon surface-enhanced enzyme-catalyzed luminol chemiluminescence at vicinity of nanoscale-corrugated gold and silver films.
Au and Ag biochips were fabricated to investigate the influence of pH upon the chemiluminescence (CL) of luminol at vicinity of surface-adsorbed peroxidase. A nanoscaled-corrugation of the metal induces an enhancement of the luminol CL which is maximal in the pH range favoring peroxidase catalysis and greater for gold than for silver. This is the proof that, in the CL process, the reactions involving peroxidase are surface-enhanced near corrugated surfaces.
Ou
Meigui
M
MATEIS, INSA-Lyon, Université de Lyon, Villeurbanne, France.
Lu
Guowei
G
Shen
Hong
H
Descamps
Armel
A
Marquette
Christophe André
CA
Blum
Loïc Jacques
LJ
Roux
Stéphane
S
Tillement
Olivier
O
Cheng
Bolin
B
Perriat
Pascal
P
D000327:Adsorption; D055162:Biocatalysis; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008165:Luminol / Q000737:chemistry* / Q000378:metabolism*; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D009195:Peroxidase / Q000378:metabolism*; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties
D008567:Membranes, Artificial; D012834:Silver; D008165:Luminol; D006046:Gold; D006861:Hydrogen Peroxide; D009195:Peroxidase