title: Cellular delivery of locked nucleic acids (LNAs).
abstract: Locked nucleic acids (LNAs) are RNA derivatives that have an O-methylene linkage between the 2 and 4 positions of the ribose. This leads to exceptionally high-affinity binding to complementary sequences. They are synthesized using standard DNA/RNA synthesis methods, and have a negatively charged backbone that confers good solubility. This unit describes a method for the introduction of LNA oligomers into cells. A support protocol also describes the determination of melting temperatures for LNA oligomers.
authors.affiliation: University of Texas Southwestern Medical Center at Dallas, USA.
authors.forename: Dwaine A
authors.initials: DA
authors.lastname: Braasch
authors.forename: David R
authors.initials: DR
authors.lastname: Corey
mesh_terms: D002463:Cell Membrane Permeability; D002477:Cells / Q000378:metabolism*; D016503:Drug Delivery Systems; D009841:Oligonucleotides / Q000493:pharmacokinetics*; D012313:RNA; D013696:Temperature
chemical_list: D009841:Oligonucleotides; C477371:locked nucleic acid; D012313:RNA