Using GFS to identify encoding genomic loci from protein mass spectral data.
Genome-based peptide fingerprint scanning (GFS) directly maps several types of protein mass spectral (MS) data to the loci in the genome that may have encoded for the protein. This process can be used either for protein identification or for proteogenomic mapping, which is gene-finding and annotation based on proteomic data. Inputs to the program are one or more mass spectrometry files from peptide mass fingerprinting and/or tandem MS (MS/MS) along with one or more sequences to search them against, and the output is the coordinates of any matches found. This unit describes the use of GFS and subsequent results analysis.
Holmes
Mark R
MR
Department of Microbiology and Immunology, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Giddings
Morgan C
MC
D000465:Algorithms; D000595:Amino Acid Sequence; D001483:Base Sequence; D002874:Chromosome Mapping; D005815:Genetic Code; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D017421:Sequence Analysis / Q000379:methods*; D012984:Software
D011506:Proteins