Measurement of lymphokine receptors.
One of the most widely studied lymphokine systems is the T lymphocyte growth factor interleukin 2 (IL-2). This unit describes two basic methods for the quantitation and biochemical characterization of IL-2 receptors. The first method employs the radioreceptor assay. Support protocols to this technique describe quantification of data via calculations of association and dissociation rates and the Scatchard plot analysis. The second approach detects cell-surface lymphokine receptors by covalently cross-linking IL-2 to its receptor. This method employs the chemical disuccinimidyl suberate (DSS) to achieve irreversible cross-linking of IL-2 to IL-2R.
Lowenthal
J W
JW
CSIRO Division of Animal Health, Parkville,Victoria, Australia.
Malek
T R
TR
Saragovi
H
H
D003432:Cross-Linking Reagents; D006801:Humans; D007376:Interleukin-2 / Q000032:analysis*; D011869:Radioligand Assay / Q000379:methods*; D015375:Receptors, Interleukin-2 / Q000032:analysis*; D011994:Recombinant Proteins / Q000032:analysis; D013388:Succinimides / Q000737:chemistry
D003432:Cross-Linking Reagents; D007376:Interleukin-2; D015375:Receptors, Interleukin-2; D011994:Recombinant Proteins; D013388:Succinimides; C019358:disuccinimidyl suberate