Lectin affinity chromatography.
This unit describes the use of lectins for preparative glycoprotein purification. Con A-Sepharose and WGA-agarose are used for convenience and availability. Instructions are given for a small-scale pilot procedure to test for lectin binding and to determine elution conditions. There are many variations on the basic procedure in the literature, but all use the same principles: bind the protein to immobilized lectin through its sugar chain, wash away unbound protein, and elute bound protein with a simple sugar that resembles the sugar ligand of the bound protein.
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La Jolla Cancer Research Foundation, La Jolla, California, USA.
D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification; D037102:Lectins / Q000737:chemistry*; D011485:Protein Binding; D014909:Wheat Germ Agglutinins / Q000737:chemistry
D006023:Glycoproteins; D037102:Lectins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A