title: The HLA-G 14bp gene polymorphism and decidual HLA-G 14bp gene expression in pre-eclamptic and normal pregnancies.
abstract: Trophoblast expression of the non-classical MHC, HLA-G, is considered essential for feto-maternal immune tolerance and successful placentation in pregnancy. The HLA-G 14bp polymorphism in the 3'-untranslated region (UTR) of the HLA-G gene has been reported to be associated with development of pre-eclampsia (PE). In this study, maternal (peripheral blood, n=54) and fetal (cord blood, n=57) HLA-G 14bp genotypes have been determined by PCR in pre-eclamptic and normal pregnancies. In addition, HLA-G 14bp gene expression in decidua basalis (n=59) was analyzed by RT-PCR. The pre-eclamptic syndrome was neither associated with the HLA-G 14bp genotype (maternal or fetal), nor with altered decidual HLA-G 14bp gene expression. Furthermore, the HLA-G 14bp mRNA expressed in decidua basalis was of fetal origin and all potential transcripts, as predicted from the fetal HLA-G 14bp genotype, were expressed. In contrast to previous findings, we found no correlation between the HLA-G 14bp polymorphism and fetal growth. In conclusion, the fetal HLA-G 14bp genotype is reflected in the decidual HLA-G mRNA splice form profile, but does not appear to be associated with increased risk for development of PE.
authors.affiliation: Department of Cancer Research and Molecular Medicine, Faculty of Medicine, Norwegian University of Science and Technology, Women and Children's Centre, Okav Kyrres gt 11, Trondheim, Norway. Ann-Charlotte.Iversen@ntnu.no
authors.forename: Ann-Charlotte
authors.initials: AC
authors.lastname: Iversen
authors.forename: Olav Toai Duc
authors.initials: OT
authors.lastname: Nguyen
authors.forename: Linda Føll
authors.initials: LF
authors.lastname: Tømmerdal
authors.forename: Irina Poliakova
authors.initials: IP
authors.lastname: Eide
authors.forename: Veslemøy Malm
authors.initials: VM
authors.lastname: Landsem
authors.forename: Nuray
authors.initials: N
authors.lastname: Acar
authors.forename: Ronny
authors.initials: R
authors.lastname: Myhre
authors.forename: Helge
authors.initials: H
authors.lastname: Klungland
authors.forename: Rigmor
authors.initials: R
authors.lastname: Austgulen
mesh_terms: D000328:Adult; D000483:Alleles; D003656:Decidua / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006680:HLA Antigens / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000097:blood / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism; D011247:Pregnancy
chemical_list: D006680:HLA Antigens; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I