title: Isolation of human basophils.
abstract: Human basophils are normally the rarest of all circulating leukocytes and thus are among the most difficult of the blood elements to purify. In this unit, basophils are isolated from leukocyte populations, resulting in preparations of cells of which 5% to 50% are basophils and the rest are lymphocytes along with neutrophils and monocytes. This technique is advantageous because of its speed and because it minimizes basophil stimulation and histamine release.
authors.affiliation: National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
authors.forename: D D
authors.initials: DD
authors.lastname: Metcalfe
mesh_terms: D001491:Basophils / Q000166:cytology* / Q000276:immunology; D002469:Cell Separation / Q000379:methods*; D006801:Humans