Establishment of permanent cell lines by Epstein-Barr virus transformation.
This basic protocol presents Epstein-Barr virus (EBV) transformation of B cells to create a permanent cell line to provide cells for genetic and DNA analysis. Virus stocks are prepared from cultures of an infected marmoset cell line in the first support protocol. Transformed cells may be analyzed immediately or frozen for future use as described in the second support protocol.
Gilbert
J
J
Duke University Medical Center, Durham, North Carolina, USA.
D000818:Animals; D001402:B-Lymphocytes / Q000821:virology; D002144:Callithrix; D002461:Cell Line, Transformed; D002472:Cell Transformation, Viral / Q000235:genetics*; D005826:Genetics, Medical; D004854:Herpesvirus 4, Human; D006801:Humans