title: In vitro signaling by MAPK and NFkappaB pathways inhibited by Yersinia YopJ.
abstract: A procedure for an in vitro signaling assay is described for the MAPK and NFkappaB pathways. The method uses a membrane-cleared lysate that contains all the soluble components required for activating these signaling cascades. The pathways can be activated by variety of molecules, including kinases, G-proteins, and E3 ligases. We demonstrate that YopJ inhibits downstream of all these activators. The in vitro signaling assay is ideal for initial biochemical studies on activators and inhibitors of the MAPK and NFkappaB pathways.
authors.affiliation: University of Texas Southwestern Medical Center, Dallas, Texas, USA.
authors.forename: Sohini
authors.initials: S
authors.lastname: Mukherjee
authors.forename: Kim
authors.initials: K
authors.lastname: Orth
mesh_terms: D001426:Bacterial Proteins / Q000494:pharmacology*; D002478:Cells, Cultured; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D016328:NF-kappa B / Q000187:drug effects* / Q000502:physiology; D015398:Signal Transduction; D048029:TNF Receptor-Associated Factor 6 / Q000502:physiology; D015007:Yersinia / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D016328:NF-kappa B; D048029:TNF Receptor-Associated Factor 6; C109227:YopP protein, Yersinia