title: [Expression of quail vascular endothelial growth factor receptor quek-1 extracellular domain 2-4 loop cDNA in Pichia pastoris].
abstract: The extracellular domain 2-4 loop cDNA of quail vascular endothelial growth factor receptor quek1 (qVEGFR2) was obtained from plasmid carrying qVEGFR2 by PCR. Then it was cloned into expression vector pPICZalphaA of Pichia pastoris. To construct recombinant expression plasmid pPICZalphaA-qVEGFR2, linearized pPICZalphaA-qVEGFR2 with SacI was transformed to electroporated Pichia pastoris GS115. Subsequently, positive clone was selected by PCR and its phenotype was determined. SDSPAGE and Western blot assays of culture medium from a methanol-induced expression strain demonstrated that recombinant qVEGFR2 proteins were expressed and secreted into the culture medium. These results could provide a basis for further researches on tumor protein vaccine as well as for the preparation of tumor protein vaccine with Pichia pastoris.
authors.affiliation: State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610041, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Diao
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Wen
authors.forename: Yongsheng
authors.initials: Y
authors.lastname: Wang
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Do
authors.forename: Hang
authors.initials: H
authors.lastname: Zhou
authors.forename: Yuquan
authors.initials: Y
authors.lastname: Wei
mesh_terms: D000818:Animals; D019496:Cancer Vaccines; D018076:DNA, Complementary / Q000096:biosynthesis* / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D010843:Pichia / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D011784:Quail; D017981:Receptors, Neurotransmitter / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000032:analysis / Q000096:biosynthesis* / Q000235:genetics; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D019496:Cancer Vaccines; D018076:DNA, Complementary; D017981:Receptors, Neurotransmitter; D011994:Recombinant Proteins; C083131:KDR protein, Coturnix japonica; D040301:Vascular Endothelial Growth Factor Receptor-2