title: Techniques for mammalian cell tissue culture.
abstract: This appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
authors.affiliation: Molecular Pathology Laboratory Network, Maryville, Tennessee.
authors.forename: Mary C
authors.initials: MC
authors.lastname: Phelan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage; D000935:Antifungal Agents / Q000008:administration & dosage; D015925:Cryopreservation; D003470:Culture Media; D006801:Humans; D008322:Mammals; D046509:Tissue Culture Techniques
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D003470:Culture Media