Peripheral blood monocytes from the decompensated liver cirrhosis could migrate into nude mouse liver with human hepatocyte-markers expression.
Adult stem cells provide a promising alternative for the treatment of decompensated liver cirrhosis. Our previous study showed that peripheral blood monocytes (PBMCs) from decompensated liver cirrhosis could differentiate into hepatocyte-like cells in vitro. We here aimed to investigate the differential potential of the PBMCs under liver environment. PBMCs were isolated from one cirrhotic patient, who was mobilized by the recombinant human granulocyte colony stimulating factor for consecutive 3days, and then PBMCs were transplanted into nude mice by tail vein after labeled with PKH26-GL. The location of the transplanted PBMCs was identified by PKH26-GL staining and PRINS for human SRY gene. The expressions of human hepatocyte-markers were detected by immunohistochemistry, RT-PCR, and Western blot analysis. Our results demonstrated that PBMCs from decompensated liver cirrhosis could migrate into the liver of nude mice with human hepatocyte-markers expression, indicating that autologous PBMCs transplantation might be one alternative therapy for the decompensated liver cirrhosis.
Yan
Li
L
Department of Gastroenterology, Xijing Hospital, The Fourth Military Medical University, 17 Changle Western Road, Xi'an, Shaanxi Province 710032, China.
Han
Ying
Y
Wang
Jingbo
J
Liu
Jingmei
J
He
Yuanlong
Y
Wang
Honghong
H
Fan
Daiming
D
D000418:Albumins / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002465:Cell Movement; D017931:DNA Primers / Q000737:chemistry; D005260:Female; D005456:Fluorescent Dyes / Q000032:analysis / Q000737:chemistry; D024282:Genes, sry; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology; D022781:Hepatocytes / Q000737:chemistry / Q000502:physiology*; D006801:Humans; D007150:Immunohistochemistry; D008099:Liver / Q000166:cytology; D008103:Liver Cirrhosis / Q000628:therapy; D051379:Mice; D008819:Mice, Nude; D009000:Monocytes / Q000187:drug effects / Q000502:physiology* / Q000637:transplantation*; D009930:Organic Chemicals / Q000032:analysis / Q000737:chemistry; D011994:Recombinant Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000737:chemistry / Q000187:drug effects / Q000502:physiology*
D000418:Albumins; D015415:Biomarkers; D017931:DNA Primers; D005456:Fluorescent Dyes; D009930:Organic Chemicals; C070080:PKH 26; D011994:Recombinant Proteins; D016179:Granulocyte Colony-Stimulating Factor