Growth retardation and death of rice plants irradiated with carbon ion beams is preceded by very early dose- and time-dependent gene expression changes.
The carbon-ion beam (CIB) generated by the heavy-ion medical accelerator in Chiba (HIMAC) was targeted to 7-day-old rice. Physiological parameters such as growth, and gene expression profiles were examined immediately after CIB irradiation. Dose-dependent growth suppression was seen three days post-irradiation (PI), and all the irradiated plants died by 15 days PI. Microarray (Agilent rice 22K) analysis of the plants immediately after irradiation (iai) revealed effects on gene expression at 270 Gy; 353 genes were up-regulated and 87 down-regulated. Exactly the same set of genes was affected at 90 Gy. Among the highly induced genes were genes involved in information storage and processing, cellular processes and signaling, and metabolism. RT-PCR analysis confirmed the microarray data.
Rakwal
Randeep
R
Human Stress Signal Research Center (HSS), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba West, 16-1 Onogawa, Tsukuba 305-8569, Japan. rakwal-68@aist.go.jp
Kimura
Shinzo
S
Shibato
Junko
J
Nojima
Kumie
K
Kim
Yeon-Ki
YK
Nahm
Baek Hie
BH
Jwa
Nam-Soo
NS
Endo
Satoru
S
Tanaka
Kenichi
K
Iwahashi
Hitoshi
H
D002244:Carbon / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D004307:Dose-Response Relationship, Radiation; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects*; D017343:Genes, Plant; D020450:Heavy Ions; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics* / Q000254:growth & development* / Q000528:radiation effects; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000254:growth & development / Q000528:radiation effects; D013997:Time Factors
D002244:Carbon