Induction of apoptosis in yeast by L-amino acid oxidase from the Malayan pit viper Calloselasma rhodostoma.
Here we report for the first time that L-amino acid oxidase (LAAO), a major component of snake venom, induces apoptosis in yeast. The causative agent for induction of apoptosis has been shown to be hydrogen peroxide, produced by the enzymatic activity of LAAO. However, the addition of catalase, a specific hydrogen peroxide scavenger, does not prevent cell demise completely. Intriguingly, depletion of leucine from the medium by LAAO and the interaction of LAAO with yeast cells are shown to be the major factors responsible for cell demise in the presence of catalase.
Ande
Sudharsana Rao
SR
Technische Universität Graz, Institut für Biochemie, Graz, Austria.
Fussi
Heike
H
Knauer
Heide
H
Murkovic
Michael
M
Ghisla
Sandro
S
Fröhlich
Kai-Uwe
KU
Macheroux
Peter
P
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002374:Catalase / Q000378:metabolism; D015169:Colony Count, Microbial; D006861:Hydrogen Peroxide / Q000494:pharmacology; D020287:In Situ Nick-End Labeling; D050830:L-Amino Acid Oxidase / Q000494:pharmacology*; D007930:Leucine / Q000378:metabolism*; D008296:Malaysia; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D014757:Viper Venoms / Q000737:chemistry* / Q000201:enzymology; D017819:Viperidae
D014757:Viper Venoms; D006861:Hydrogen Peroxide; D002374:Catalase; D050830:L-Amino Acid Oxidase; D007930:Leucine