title: The value of occult disease in resection margin and lymph node after extrapleural pneumonectomy for malignant mesothelioma.
abstract: BACKGROUND
  The purpose of this study was to examine the prognostic impact of occult disease after extrapleural pneumonectomy for malignant mesothelioma.
  
  
  METHODS
  We reviewed the resection margin and node specimens from 41 consecutive patients undergoing extrapleural pneumonectomy for malignant pleural mesothelioma in different institutions between 1985 and 2004. The specimens were reassessed by immunohistochemical staining with anticalretinin and antimesothelin monoclonal antibodies, and results were used to draw Kaplan-Meier survival curves and perform Cox regression analyses.
  
  
  RESULTS
  Histologic examination showed 34 epithelioid, 4 biphasic, and 3 sarcomatoid subtypes. Results of postoperative TNM staging were that 14 patients were in stage I, 6 were in stage II, and 21 were in stage III. One patient died during the early postoperative period. Median survival was 13 months. Survival was affected by nonepithelial histologic type (p = 0.001), TNM stage (p = 0.007), positive resection margins (p = 0.002), and N disease (p = 0.01). Immunohistochemistry revealed occult positive resection margins in 6 patients, not correlated with T stage. Microscopic N disease was discovered in 5 patients, of whom 2 had their nodes retrieved through cervical mediastinoscopy. No correlation with nodal diameter was found. In all patients microscopic N disease could have been accessible through mediastinoscopy. Overall, the presence of occult disease was diagnosed in 5 new patients and influenced survival more than any other variable, both at univariate (p < 0.001) and multivariate Cox regression analysis (p < 0.0001; odds ratio, 5.4; 95% confidence interval, 3 to 15).
  
  
  CONCLUSIONS
  In malignant pleural mesothelioma, the presence of occult disease in resection margins and lymph nodes can be identified by immunohistochemistry and significantly influences the prognosis. Cervical mediastinoscopy is useful in all patients considered for radical resection, but all specimens should be processed with immunohistochemical staining.
authors.affiliation: Department of Thoracic Surgery, Policlinico Tor Vergata University, Rome, Italy. mineo@med.uniroma2.it
authors.forename: Tommaso Claudio
authors.initials: TC
authors.lastname: Mineo
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Ambrogi
authors.forename: Eugenio
authors.initials: E
authors.lastname: Pompeo
authors.forename: Alfonso
authors.initials: A
authors.lastname: Baldi
authors.forename: Franco
authors.initials: F
authors.lastname: Stella
authors.forename: Paolo
authors.initials: P
authors.lastname: Aurea
authors.forename: Mario
authors.initials: M
authors.lastname: Marino
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D064032:Calbindin 2; D005260:Female; D005500:Follow-Up Studies; D058851:GPI-Linked Proteins; D006801:Humans; D053208:Kaplan-Meier Estimate; D008197:Lymph Node Excision; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis / Q000473:pathology*; D008297:Male; D008481:Mediastinoscopy; D008562:Membrane Glycoproteins / Q000032:analysis; D000090204:Mesothelin; D008654:Mesothelioma / Q000401:mortality / Q000473:pathology / Q000601:surgery*; D008875:Middle Aged; D009367:Neoplasm Staging; D018365:Neoplasm, Residual / Q000401:mortality / Q000473:pathology*; D010997:Pleural Neoplasms / Q000401:mortality / Q000473:pathology / Q000601:surgery*; D011013:Pneumonectomy; D011379:Prognosis; D012044:Regression Analysis; D012189:Retrospective Studies; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D015996:Survival Rate
chemical_list: D014408:Biomarkers, Tumor; D064032:Calbindin 2; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D064030:S100 Calcium Binding Protein G; D000090204:Mesothelin