Characterization of human DNA in environmental samples.
Environmental samples from indoor surfaces can be confounded by dust, which is composed largely of human skin cells and has been documented to contain roughly tens of micrograms of total DNA per gram of dust. This study complements previous published work by providing estimates of the quantity of amplifiable human DNA found in environmental samples from a typical indoor environment, categorized by the intensity of human traffic and visible quantity of dust. Dust was collected by surface swabbing standard 576 cm(2) areas in eight locations, and evaluated for total DNA quantity, presence of human DNA (mitochondrial and nuclear loci using conventional PCR), quantity of human nuclear DNA using quantitative PCR, and STR analysis. The total DNA content of 36 dust samples ranged from 9 to 28 ng/cm(2), and contained 0.2-1.1 pg/cm(2) of human DNA. Overall, human DNA was detected in 97% of 36 dust samples and 61% of samples yielded allele distributions of varying degrees of complexity when subjected to STR analysis. The implications of this study are twofold. First, the presence of dust in evidence can be a significant contamination source in forensic investigations because the human DNA component is of sufficient quality and quantity to produce allele calls in STR analysis. This can be effectively managed by implementing stringent protocols for collection and analysis of potential biological samples. A second implication is the use of dust as a source of evidence for identification of inhabitants within a defined location. In the latter case, a number of additional studies would be necessary to identify relevant pretreatments for environmental dust samples and to develop the necessary deconvolution techniques to separate the composite genotypes obtained.
Toothman
Mary H
MH
Department of Biology, Virginia Commonwealth University, 1000 W. Cary Street, Richmond, VA 23284-2012, USA.
Kester
Karen M
KM
Champagne
Jarrod
J
Cruz
Tracey Dawson
TD
Street
W Scott
WS
Brown
Bonnie L
BL
D004247:DNA / Q000032:analysis*; D004391:Dust; D004777:Environment; D053803:Forensic Genetics; D005787:Gene Frequency; D006801:Humans; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
D004391:Dust; D004247:DNA