title: Introduction of plasmid DNA into cells.
abstract: This appendix presents a procedure for transformation using calcium chloride, and also an alternate procedure for one-step preparation and transformation of competent cells.
authors.affiliation: Harvard Medical School, Boston, Massachusetts, USA.
authors.forename: C E
authors.initials: CE
authors.lastname: Seidman
authors.forename: K
authors.initials: K
authors.lastname: Struhl
mesh_terms: D002122:Calcium Chloride; D004247:DNA / Q000378:metabolism*; D004926:Escherichia coli; D018014:Gene Transfer Techniques; D010957:Plasmids / Q000378:metabolism*; D014169:Transformation, Bacterial
chemical_list: D004247:DNA; D002122:Calcium Chloride