title: Physiological effects of human muscle-derived stem cell implantation on urethral smooth muscle function.
abstract: The physiological effects of human muscle-derived stem cell (MDSC) implantation on urethral smooth muscle function were investigated in pudendal nerve-transected nude rats with human MDSC (TM) or saline (TS) injection into the proximal urethra compared with sham-operated, saline-injected nude rats (SS). Leak point pressure (LPP) before and after hexamethonium application, which can block autonomic efferent nerves, and proximal urethral contractile responses to carbachol and phenylephrine in muscle strip study were examined 6 weeks after the implantation. There was no significant difference between the LPPs in SS and TM. Following hexamethonium application, the LPP in TM was, however, significantly decreased compared with SS. The contractile responses to phenylephrine, but not to carbachol, in TM were significantly increased compared with SS and TS. These results suggest that the restorative effects of MDSCs are mediated by autonomic nerves and that increased sensitivity of alpha(1)-adrenoceptors may be related to restore the deficient urethral function.
authors.affiliation: Department of Urology, University of Pittsburgh School of Medicine, 3471 Fifth Avenue, Pittsburgh, PA 15213, USA.
authors.forename: Akira
authors.initials: A
authors.lastname: Furuta
authors.forename: Ron J
authors.initials: RJ
authors.lastname: Jankowski
authors.forename: Ryan
authors.initials: R
authors.lastname: Pruchnic
authors.forename: Shin
authors.initials: S
authors.lastname: Egawa
authors.forename: Naoki
authors.initials: N
authors.lastname: Yoshimura
authors.forename: Michael B
authors.initials: MB
authors.lastname: Chancellor
mesh_terms: D000818:Animals; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000502:physiology*; D051381:Rats; D011923:Rats, Nude; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation; D014521:Urethra / Q000502:physiology*; D014549:Urinary Incontinence / Q000628:therapy*