title: Preparation and maintenance of organotypic cultures for multi-electrode array recordings.
abstract: Recording from neuronal cultures with multi-electrode arrays (MEAs) provides a powerful tool for studying neuronal activity with many neurons simultaneously in vitro. This unit describes the detailed steps necessary for growing organotypic cultures on MEAs and the typical neuronal activity that is obtained with this methodology.
authors.affiliation: Laboratory of Systems Neuroscience, NIMH, NIH, Bethesda, Maryland, USA.
authors.forename: Veronica C
authors.initials: VC
authors.lastname: Karpiak
authors.forename: Dietmar
authors.initials: D
authors.lastname: Plenz
mesh_terms: D000818:Animals; D008839:Microelectrodes; D009474:Neurons / Q000502:physiology; D009924:Organ Culture Techniques / Q000379:methods*; D018408:Patch-Clamp Techniques / Q000379:methods*