title: Estimation of chromosomal imbalances in preimplantation embryos from preimplantation genetic diagnosis cycles of reciprocal translocations with or without acrocentric chromosomes.
abstract: OBJECTIVE
  To estimate chromosomal imbalances in preimplantation embryos from reciprocal translocation carriers with or without acrocentric chromosomes (Acro-Ch) 13, 14, 15, 21, and 22 in preimplantation genetic diagnosis (PGD) cycles.
  
  
  METHODS
  Fluorescence in situ hybridization was applied to PGD cycles for reciprocal translocation carriers.
  
  
  METHODS
  University-based centers for reproductive medicine.
  
  
  METHODS
  Ten and 24 patients of reciprocal translocation with and without Acro-Ch, respectively.
  
  
  METHODS
  Fluorescence in situ hybridization in biopsied blastomeres.
  
  
  METHODS
  Estimation of meiotic segregation mode in embryos from translocation carriers.
  
  
  RESULTS
  The proportion of alternative segregation for normal or balanced chromosome contents in preimplantation embryos from PGD cycles in reciprocal translocations without Acro-Ch was significantly higher than that with Acro-Ch (26.0% vs. 14.6%). The proportion of interchange trisomy in 3:1 segregation was significantly lower in reciprocal translocations without Acro-Ch than that with Acro-Ch (4.3% vs. 9.5%).
  
  
  CONCLUSIONS
  This is the first report that the incidence of alternative segregation producing normal or balanced embryos was relatively low in reciprocal translocations associated with Acro-Ch. Our data may be useful to predict the possibility of normal or balanced embryos and to counsel with reciprocal translocation carriers for PGD-fluorescence in situ hybridization cycles.
authors.affiliation: Laboratory of Reproductive Biology and Infertility, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine, Seoul, South Korea.
authors.forename: Chun Kyu
authors.initials: CK
authors.lastname: Lim
authors.forename: Jae Won
authors.initials: JW
authors.lastname: Cho
authors.forename: In Ok
authors.initials: IO
authors.lastname: Song
authors.forename: Inn Soo
authors.initials: IS
authors.lastname: Kang
authors.forename: Yong-Dal
authors.initials: YD
authors.lastname: Yoon
authors.forename: Jin Hyun
authors.initials: JH
authors.lastname: Jun
mesh_terms: D000022:Abortion, Spontaneous / Q000209:etiology; D000328:Adult; D020090:Chromosome Segregation; D002877:Chromosomes, Human; D004624:Embryo Transfer; D005260:Female; D005820:Genetic Testing; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007246:Infertility / Q000628:therapy*; D007621:Karyotyping; D007723:Korea; D050498:Live Birth; D011247:Pregnancy; D018873:Pregnancy Rate; D019836:Preimplantation Diagnosis / Q000379:methods*; D027724:Reproductive Techniques, Assisted / Q000009:adverse effects; D012189:Retrospective Studies; D014178:Translocation, Genetic; D016896:Treatment Outcome