[Analysis of the variation of 32 HIV-1 V3 loop from infected Chinese blood/plasma donors].
OBJECTIVE
To characterize the variation in V3 loop of HIV-1 B'strains circulating in Chinese blood donors.


METHODS
The c2-c3 regions of the HIV envelop gene were amplified by nest-PCR from 32 HIV-1-infected blood donors in He Nan province in China. The BIOEDIT and MEGA software are used to analyze the sequences of V3 loop.


RESULTS
There are five types of central motifs of the 32 samples, in which GPGR and GPGQ are most common. More variations associated with T tropic/SI phenotype can be seen in AIDS group.


CONCLUSIONS
The V3 tip motifs of HIV-1B' strains circulating in Chinese blood donors are various, the different characterization of V3 loop between AIDS and asymptomatic patients indicates different biological phenotype and pathogenesis which warrant additional investigation.
Dai
Lli
L
Department of Infectious Disease. The You An Hospital affiliated to The Capital University of Medical Science. Beijing, 100069, China.
Lu
Jun-feng
JF
Huang
Xiao-jie
XJ
Shi
Ying
Y
Chen
De-xi
DX
Wu
Hao
H
D000328:Adult; D000368:Aged; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001782:Blood Donors; D002681:China; D005260:Female; D014644:Genetic Variation / Q000235:genetics*; D015699:HIV Envelope Protein gp120 / Q000032:analysis / Q000737:chemistry* / Q000235:genetics*; D015658:HIV Infections / Q000097:blood / Q000821:virology*; D015497:HIV-1 / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000737:chemistry; D010641:Phenotype
D015699:HIV Envelope Protein gp120; D009842:Oligopeptides