title: Hydrophobic-interaction chromatography.
abstract: This unit discusses the important parameters in designing and optimizing a separation by hydrophobic interaction chromatography (HIC), including preparing the sample and choosing a matrix, column, and buffer. Protocols are provided for packing and testing a column; determining binding and elution conditions; eluting the sample; and cleaning, regenerating, and storing HIC columns.
authors.affiliation: Pharmacia Biotech, Piscataway, New Jersey, USA.
authors.forename: R M
authors.initials: RM
authors.lastname: Kennedy
mesh_terms: D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011506:Proteins