Delayed-type hypersensitivity.
Delayed-type hypersensitivity (DTH) is an in vivo assay of cell-mediated immune function. DTH reactions are often divided into two phases: the sensitization phase, referring to the initial immunization with specific antigen, and the efferent or challenge phase of the DTH response, which usually follows 6 to 14 days after sensitization. This unit details the protocol for stimulating DTH responses in mice to the O-succinimide ester of the hapten 4-hydroxy-3-nitrophenyl acetyl (abbreviated NP-O-Su).
Luo
Y
Y
Harvard Medical School, Boston, Massacusetts, USA.
Dorf
M E
ME
D000818:Animals; D006241:Haptens / Q000276:immunology*; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D007111:Immunity, Cellular; D007158:Immunologic Techniques; D051379:Mice; D009596:Nitrophenols / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology
D006241:Haptens; D009596:Nitrophenols; C026654:4-hydroxy-3-nitrophenylacetyl-O-succinimide ester