title: Accumulation of chondroitin sulfate proteoglycans in the microenvironment of spinal motor neurons in amyotrophic lateral sclerosis transgenic rats.
abstract: Chondroitin sulfate proteoglycans (CSPGs) are the major components of extracellular matrix in the central nervous system. In the spinal cord under various types of injury, reactive gliosis emerges in the lesion accompanied by CSPG up-regulation. Several types of CSPG core proteins and their side chains have been shown to inhibit axonal regeneration in vitro and in vivo. In the present study, we examined spatiotemporal expression of CSPGs in the spinal cord of transgenic (Tg) rats with His46Arg mutation in the Cu/Zn superoxide dismutase gene, a model of amyotrophic lateral sclerosis (ALS). Immunofluorescence disclosed a significant up-regulation of neurocan, versican, and phosphacan in the ventral spinal cord of Tg rats compared with age-matched controls. Notably, Tg rats showed progressive and prominent accumulation of neurocan even at the presymptomatic stage. Immunoblotting confirmed the distinct increase in the levels of both the full-length neurocan and their fragment isoforms. On the other hand, the up-regulation of versican and phosphacan peaked at the early symptomatic stage, followed by diminishment at the late symptomatic stage. In addition, double immunofluorescence revealed a colocalization between reactive astrocytes and immunoreactivities for neurocan and phosphacan, especially around residual large ventral horn neurons. Thus, reactive astrocytes are suggested to be participants in the CSPG accumulation. Although the possible neuroprotective involvement of CSPG remains to be investigated, the present results suggest that both the reactive astrocytes and the differential accumulation of CSPGs may create a nonpermissive microenvironment for neural regeneration in neurodegenerative diseases such as ALS.
authors.affiliation: Division of Neurology, Department of Neuroscience, Tohoku University Graduate School of Medicine, Tohoku University Hospital ALS Center, Sendai, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Mizuno
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Warita
authors.forename: Masashi
authors.initials: M
authors.lastname: Aoki
authors.forename: Yasuto
authors.initials: Y
authors.lastname: Itoyama
mesh_terms: D000690:Amyotrophic Lateral Sclerosis / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D030801:Animals, Genetically Modified; D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western; D011508:Chondroitin Sulfate Proteoglycans / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D005455:Fluorescent Antibody Technique; D007091:Image Processing, Computer-Assisted; D009046:Motor Neurons / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D013116:Spinal Cord / Q000378:metabolism / Q000473:pathology*
chemical_list: D011508:Chondroitin Sulfate Proteoglycans