title: Formation of metallic copper nanoparticles at the soil-root interface.
abstract: Copper is an essential element in the cellular electron-transport chain, but as a free ion it can catalyze production of damaging radicals. Thus, all life forms attempt to prevent copper toxicity. Plants diminish excess copper in two structural regions: rare hyperaccumulators bind cationic copper to organic ligands in subaerial tissues, whereas widespread metal-tolerant plants segregate copper dominantly in roots by mechanisms thought to be analogous. Here we show using synchrotron microanalyses that common wetlands plants Phragmites australis and Iris pseudoacorus can transform copper into metallic nanoparticles in and near roots with evidence of assistance by endomycorrhizal fungi when grown in contaminated soil in the natural environment. Biomolecular responses to oxidative stress, similar to reactions used to abiotically synthesize Cu0 nanostructures of controlled size and shape, likely cause the transformation. This newly identified mode of copper biomineralization by plant roots under copper stress may be common in oxygenated environments.
authors.affiliation: LGIT-Maison des Géosciences, CNRS and Université J. Fourier, 38041 Grenoble 9, France. manceau@obs.ujf-grenoble.fr
authors.forename: Alain
authors.initials: A
authors.lastname: Manceau
authors.forename: Kathryn L
authors.initials: KL
authors.lastname: Nagy
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Marcus
authors.forename: Martine
authors.initials: M
authors.lastname: Lanson
authors.forename: Nicolas
authors.initials: N
authors.lastname: Geoffroy
authors.forename: Thierry
authors.initials: T
authors.lastname: Jacquet
authors.forename: Tatiana
authors.initials: T
authors.lastname: Kirpichtchikova
mesh_terms: D003300:Copper / Q000737:chemistry*; D005453:Fluorescence; D053768:Metal Nanoparticles; D010084:Oxidation-Reduction; D018517:Plant Roots / Q000737:chemistry*; D012987:Soil; D013057:Spectrum Analysis / Q000379:methods; D053833:Wetlands; D014965:X-Rays
chemical_list: D012987:Soil; D003300:Copper