title: Sequential production of amylolytic and lipolytic enzymes by bacterium strain isolated from petroleum contaminated soil.
abstract: Amylases and lipases are highly demanded industrial enzymes in various sectors such as food, pharmaceuticals, textiles, and detergents. Amylases are of ubiquitous occurrence and hold the maximum market share of enzyme sales. Lipases are the most versatile biocatalyst and bring about a range of bioconversion reactions such as hydrolysis, inter-esterification, esterification, alcoholysis, acidolysis, and aminolysis. The objective of this work was to study the feasibility for amylolitic and lipolytic production using a bacterium strain isolated from petroleum contaminated soil in the same submerged fermentation. This was a sequential process based on starch and vegetable oils feedstocks. Run were performed in batchwise using 2% starch supplemented with suitable nutrients and different vegetable oils as a lipase inducers. Fermentation conditions were pH 5.0; 30 degrees C, and stirred speed (200 rpm). Maxima activities for amyloglucosidase and lipase were, respectively, 0.18 and 1,150 U/ml. These results showed a promising methodology to obtain both enzymes using industrial waste resources containing vegetable oils.
authors.affiliation: Universidade Tiradentes, Avenida Murilo Dantas, 300-Farolândia, Aracaju, SE, Brazil.
authors.forename: Nayara Bezerra
authors.initials: NB
authors.lastname: Carvalho
authors.forename: Ranyere Lucena
authors.initials: RL
authors.lastname: de Souza
authors.forename: Heizir F
authors.initials: HF
authors.lastname: de Castro
authors.forename: Gisella M
authors.initials: GM
authors.lastname: Zanin
authors.forename: Alvaro Silva
authors.initials: AS
authors.lastname: Lima
authors.forename: Cleide M F
authors.initials: CM
authors.lastname: Soares
mesh_terms: D000681:Amylases / Q000096:biosynthesis; D001419:Bacteria / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D004787:Environmental Pollution; D008049:Lipase / Q000096:biosynthesis; D010578:Petroleum / Q000633:toxicity*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity
chemical_list: D010578:Petroleum; D012989:Soil Pollutants; D008049:Lipase; D000681:Amylases