Luminescent detection of ATP in aqueous solution using positively charged CdSe-ZnS quantum dots.
Commercially available CdSe-ZnS Quantum Dots (QDs) have been modified by exchanging the hydrophobic surface ligands with (2-mercaptoethyl)-trimethylammonium chloride. The resulting water soluble conjugate was titrated with solutions of adenosine triphosphate (ATP), adenosine diphosphate, adenosine monophosphate, guanosine triphosphate (GTP), guanosine diphosphate and guanosine monophosphate in 0.01 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer (pH 7.4). A strong fluorescence quench of about 80% was observed for ATP, a quench of 25% was observed for GTP while the others had virtually no effect. The quenching effect of ATP and GTP was attributed to the high negative charge density associated with these substrate's resulting in a strong attraction to the QD surface enabling them to engage in electron transfer with the excited QD. The lack of fluorescence quenching associated with the other nucleotides was most likely due to their reduced charge density resulting in a lower affinity for the QD surface.
Callan
John F
JF
School of Pharmacy, The Robert Gordon University, Aberdeen, Scotland, AB10 1FR, UK. j.callan@rgu.ac.uk
Mulrooney
Ray C
RC
Kamila
Sukanta
S
D000255:Adenosine Triphosphate / Q000032:analysis*; D019187:Cadmium Compounds / Q000737:chemistry*; D005453:Fluorescence; D008163:Luminescent Measurements / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy; D045663:Quantum Dots; D018036:Selenium Compounds / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D013499:Surface Properties; D014867:Water / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
D019187:Cadmium Compounds; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D014867:Water; D000255:Adenosine Triphosphate; C058667:cadmium selenide; C031238:zinc sulfide