Quantitative amino acid analysis.
Colorimetric dye-binding assays are often used to determine protein concentration, however, many proteins do not bind dyes in the same way as the proteins normally used as standards, making quantitation by those techniques difficult. This unit describes the information that can be derived from quantitative amino acid analysis (including precise determination of protein concentration), presents some details on sample preparation, and gives examples of the calculations that are needed.
Dunn
B
B
University of Florida, Gainesville, Florida, USA.
D000596:Amino Acids / Q000032:analysis*; D011506:Proteins / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods
D000596:Amino Acids; D011506:Proteins