title: Influence of substance-P on cultured sebocytes.
abstract: Acne is a complex, chronic and common skin disorder of pilosebaceous units. Although it is known that exacerbation of acne results from emotional stress, the nature of the association between stress and acne remains unclear. This is due in part to the lack of substantial evidence regarding the participation of cutaneous neurogenic factors in the pathogenesis of acne. Culture of sebocytes provides a new insight into the participation of neuropeptides, notably substance P (SP), in the pathophysiology of acne. To examine the possible involvement of neurogenic factors in the pathogenesis of acne, we used immunohistochemistry and RT-PCR to compare the expression of interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), peroxisome proliferators activated receptors-gamma (PPAR-gamma) on the cultured sebocytes stimulated by SP. IL-1 is primarily proinflammatory cytokines to stimulate the expression of genes associated with inflammation. IL-6 is a pleiotropic cytokine with a wide range of biological activities and regulates inflammation. TNF-alpha is a pleiotropic pro-inflammatory cytokine that exerts multiple biologic effects. PPAR-gamma is a nuclear hormone receptor and plays a unique role in stimulating sebocyte lipogenesis. More numerous immunoreactivity to IL-1, IL-6, TNF-alpha and PPAR-gamma and increased RNA amplification for IL-1, IL-6, TNF-alpha and PPAR-gamma were observed after addition of SP compared with the control. This study reveals that SP is involved in the pathogenesis of acne.
authors.affiliation: Department of Dermatology, Kyungpook National University School of Medicine, Jung-gu, Daegu, Republic of Korea. weonju@knu.ac.kr
authors.forename: Weon Ju
authors.initials: WJ
authors.lastname: Lee
authors.forename: Hong Dae
authors.initials: HD
authors.lastname: Jung
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Byung Soo
authors.initials: BS
authors.lastname: Kim
authors.forename: Seok-Jong
authors.initials: SJ
authors.lastname: Lee
authors.forename: Do Won
authors.initials: DW
authors.lastname: Kim
mesh_terms: D000152:Acne Vulgaris / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007249:Inflammation; D050155:Lipogenesis / Q000276:immunology; D015213:Neuroimmunomodulation / Q000276:immunology; D047495:PPAR gamma / Q000276:immunology* / Q000378:metabolism; D012627:Sebaceous Glands / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D012629:Sebum / Q000276:immunology / Q000378:metabolism; D013373:Substance P / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D016207:Cytokines; D047495:PPAR gamma; D013373:Substance P