title: Presence of fatty-acid-binding protein 4 expression in human epicardial adipose tissue in metabolic syndrome.
abstract: BACKGROUND
  Metabolic syndrome is a cluster of different clinical manifestations that are risk factors for atherothrombotic cardiovascular disorders. Fatty-acid-binding protein 4 (FABP4/aP2), which is highly expressed in adipocytes, specifically exerts intracellular lipid trafficking. A high level of fatty-acid-binding protein 4 expression present in obese subjects has also been found in mice and humans, especially in macrophages at atherosclerotic lesions. An in vivo study demonstrated that the inhibitor of aP2 would be a new therapeutic agent for treating metabolic diseases in mice. We have investigated the mRNA expression of fatty-acid-binding protein 4 in human epicardial adipose and ascending aorta tissues of metabolic syndrome and nonmetabolic syndrome patients.
  
  
  METHODS
  Paired epicardial adipose and ascending aorta tissue samples were obtained from 10 metabolic syndrome patients and 4 nonmetabolic syndrome patients during coronary bypass grafting and aortic valve replacement therapy, respectively. Fatty-acid-binding protein 4 gene expression was determined by quantitative real-time polymerase chain reaction.
  
  
  CONCLUSIONS
  Fatty-acid-binding protein 4 expression of epicardial adipose tissue was significantly higher in metabolic syndrome patients than in nonmetabolic syndrome controls (P<.05). In metabolic syndrome patients, fatty-acid-binding protein 4 expression in epicardial adipose tissue was 66 times higher than fatty-acid-binding protein 4 expression in ascending aorta tissue. The expression level of fatty-acid-binding protein 4 in epicardial adipose tissue was found to be significantly correlated with waist circumference in all subjects (r=.535, P<.05). Our data showed for the first time that human epicardial adipose and ascending aorta tissues express fatty-acid-binding protein 4 and that its level of expression in epicardial adipose tissues of metabolic syndrome patients is elevated. Increased fatty-acid-binding protein 4 gene expression in epicardial adipose tissues of metabolic syndrome patients led us think that fatty-acid-binding protein 4 might be an important factor in metabolic syndrome.
authors.affiliation: Department of Genetics, Institute for Experimental Medicine, Istanbul University, Istanbul, Turkey. vburcak@istanbul.edu.tr
authors.forename: Burcak
authors.initials: B
authors.lastname: Vural
authors.forename: Fatmahan
authors.initials: F
authors.lastname: Atalar
authors.forename: Cavlan
authors.initials: C
authors.lastname: Ciftci
authors.forename: Ayse
authors.initials: A
authors.lastname: Demirkan
authors.forename: Belgin
authors.initials: B
authors.lastname: Susleyici-Duman
authors.forename: Demet
authors.initials: D
authors.lastname: Gunay
authors.forename: Belhhan
authors.initials: B
authors.lastname: Akpinar
authors.forename: Ertan
authors.initials: E
authors.lastname: Sagbas
authors.forename: Ugur
authors.initials: U
authors.lastname: Ozbek
authors.forename: Ahmet Sevim
authors.initials: AS
authors.lastname: Buyukdevrim
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D001011:Aorta / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000096:biosynthesis*; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D024821:Metabolic Syndrome / Q000378:metabolism*; D008875:Middle Aged; D010496:Pericardium / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins; D012333:RNA, Messenger