Tat-regulated expression of RNA interference: triggers for the treatment of HIV infection.
HIV infection is a lifelong problem requiring continual medication for suppressing viral replication. Current strategies of antiretroviral drug combinations have proven effective in prolonging the time from infection to the symptoms of AIDS. Nevertheless, chemotherapy is not without its problems, which include toxicities and eventual emergence of virus mutants that are resistant to current antiretrovirals. Gene therapy refers to the introduction of effector oligonucleotides to transiently or stably alter gene expression or the delivery and expression of an exogenous gene within a specific target cell. A number of studies have demonstrated effective silencing/inhibition of HIV-1 replication by using RNA-based effector oligonucleotides for RNA interference. In this study, we have taken advantage of lentiviral vector-mediated delivery of anti-HIV short hairpin RNA for the treatment of HIV infection in hematopoietic cells.
Unwalla
Hoshang
H
Division of Molecular Biology, Beckman Research Institute of the City of Hope, 1450 East Duarte Road, Duarte, CA 90101, USA.
Rossi
John J
JJ
D002460:Cell Line; D002478:Cells, Cultured; D005822:Genetic Vectors; D015658:HIV Infections / Q000628:therapy*; D016325:HIV Long Terminal Repeat / Q000235:genetics; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000821:virology*; D006801:Humans; D016086:Lentivirus / Q000235:genetics; D011401:Promoter Regions, Genetic; D034622:RNA Interference / Q000187:drug effects*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D013601:T-Lymphocytes / Q000821:virology; D014161:Transduction, Genetic; D054322:tat Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology*
D018840:HSP70 Heat-Shock Proteins; D034741:RNA, Small Interfering; D012367:RNA, Viral; D054322:tat Gene Products, Human Immunodeficiency Virus