title: Lectin affinity chromatography.
abstract: This unit describes the use of lectins for preparative glycoprotein purification. Con A-Sepharose and WGA-agarose are used for convenience and availability. Instructions are given for a small-scale pilot procedure to test for lectin binding and to determine elution conditions. There are many variations on the basic procedure in the literature, but all use the same principles: bind the protein to immobilized lectin through its sugar chain, wash away unbound protein, and elute bound protein with a simple sugar that resembles the sugar ligand of the bound protein.
authors.affiliation: La Jolla Cancer Research Foundation, La Jolla, California, USA.
authors.forename: H H
authors.initials: HH
authors.lastname: Freeze
mesh_terms: D002846:Chromatography, Affinity / Q000379:methods*; D003208:Concanavalin A / Q000737:chemistry; D006023:Glycoproteins / Q000737:chemistry / Q000302:isolation & purification; D037102:Lectins / Q000737:chemistry*; D011485:Protein Binding; D014909:Wheat Germ Agglutinins / Q000737:chemistry
chemical_list: D006023:Glycoproteins; D037102:Lectins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A