Hormone-induced cortical maturation ensures the slow block to polyspermy and does not couple with meiotic maturation in starfish.
Meiotic progression in starfish oocytes is reinitiated by a maturation-inducing hormone called 1-methyladenine (1-MeAde). In addition to meiotic maturation, 1-MeAde induces cortical maturation in which cortical granules become competent to discharge in response to fusion of a single sperm, which results in the formation of the fertilization envelope. We found that subthreshold concentrations of 1-MeAde induce cortical maturation without germinal vesicle breakdown (GVBD). During cortical maturation, the IP3 sensitivity of calcium stores was increased as well as during meiotic maturation. When oocytes were exposed with 1-MeAde only on a hemisphere of oocytes, the IP3 sensitivity of the cortical region was increased only in the exposed hemisphere, suggesting that signals and components involved in cortical maturation do not readily spread in the cytoplasm. Although a specific inhibitor of phosphatidylinositol-3 kinase, LY294002 blocked both GVBD and cortical maturation, a Cdc2 kinase inhibitor, roscovitine did not block cortical maturation. Inhibition of Akt activation by injecting the competitors for Akt phosphorylation and membrane recruitment also blocked cortical maturation. These results suggest that the signaling pathway leading to Akt activation is common in cortical maturation and meiotic maturation, and Cdc2 activation was not required for cortical maturation.
Hirohashi
Noritaka
N
Genetic Counseling Program, Graduate School of Humanities and Sciences, Ochanomizu University, 2-1-1 Otsuka, Bunkyo, Tokyo 112-8610, Japan. nhiro@cc.ocha.ac.jp
Harada
Kaori
K
Chiba
Kazuyoshi
K
D000199:Actins / Q000378:metabolism; D000225:Adenine / Q000031:analogs & derivatives* / Q000378:metabolism; D000818:Animals; D016203:CDC2 Protein Kinase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D004789:Enzyme Activation; D005260:Female; D005306:Fertilization / Q000502:physiology; D015544:Inositol 1,4,5-Trisphosphate / Q000378:metabolism; D008297:Male; D008540:Meiosis / Q000502:physiology*; D009865:Oocytes / Q000166:cytology / Q000502:physiology; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology; D013215:Starfish
D000199:Actins; C008407:1-methyladenine; D015544:Inositol 1,4,5-Trisphosphate; D051057:Proto-Oncogene Proteins c-akt; D016203:CDC2 Protein Kinase; D000225:Adenine; D002118:Calcium