Measurement of antiviral activity induced by interferons alpha, beta, and gamma.
The Basic Protocol in this unit describes an assay for murine IFN-induced antiviral activity and employs vesicular stomatitis virus (VSV) and IFN-sensitive fibroblasts. Support Protocol describes the preparation of VSV cultures and the calculation of multiplicity of infection (MOI; i.e., concentration of viral particles required to infect cells). Support Protocol describes the antibody neutralization assay, which can be used to identify the bioactive species of IFN in a sample, or to test the potency of an antibody preparation against a particular species of IFN. Alternate Protocol 1 covers measurement of human IFN-induced antiviral activity; while the steps are quite similar to Basic Protocol, different viral and cell cultures are described.
Vogel
S N
SN
Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.
Friedman
R M
RM
Hogan
M M
MM
D000818:Animals; D003588:Cytopathogenic Effect, Viral; D004686:Encephalomyocarditis virus / Q000276:immunology*; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D016898:Interferon-alpha / Q000276:immunology; D016899:Interferon-beta / Q000276:immunology; D007371:Interferon-gamma / Q000276:immunology; D007372:Interferons / Q000276:immunology*; D051379:Mice; D009500:Neutralization Tests / Q000379:methods*; D014721:Vesicular stomatitis Indiana virus / Q000276:immunology*
D016898:Interferon-alpha; D016899:Interferon-beta; D007371:Interferon-gamma; D007372:Interferons