title: Analysis of Helicobacter pylori isolates from Chile: occurrence of selective type 1 Lewis b antigen expression in lipopolysaccharide.
abstract: Previous studies have shown that the LPS of Helicobacter pylori isolated from North American and European hosts predominantly expresses type 2 Lewis x (Le(x)) and Le(y) epitopes, whilst the LPS from Asian strains has the capacity to express type 1 Le(a) and Le(b) structures. The aim of this study was to evaluate the expression of Le antigens and the cytotoxin-associated antigen (CagA) by H. pylori isolates from Chile. A total of 38 isolates were screened. The expression of Le antigens and CagA was determined by whole-cell indirect ELISA, using commercially available monoclonal anti-Le and polyclonal anti-CagA antibodies. LPS profiles of H. pylori isolates were assessed by gel electrophoresis and Western blotting. Expression of Le(x) and/or Le(y) epitopes was confirmed in 32/38 isolates (84 %), whilst 9/38 isolates (24 %) expressed type 1 Le(b) blood group determinants, in addition to type 2 Le(x) and Le(y) structures. Six strains (16 %) were non-typeable. The majority of H. pylori strains examined were CagA-positive (83.3 %).
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada.
authors.forename: Eleonora
authors.initials: E
authors.lastname: Altman
authors.affiliation: Instituto de Microbiología Clínica, Edificio de Ciencias Biomédicas, Facultad de Medicina, Universidad Austral de Chile, PO Box 567, Valdivia, Chile.
authors.forename: Heriberto
authors.initials: H
authors.lastname: Fernández
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada.
authors.forename: Vandana
authors.initials: V
authors.lastname: Chandan
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON K1A 0R6, Canada.
authors.forename: Blair A
authors.initials: BA
authors.lastname: Harrison
authors.affiliation: Instituto de Microbiología Clínica, Edificio de Ciencias Biomédicas, Facultad de Medicina, Universidad Austral de Chile, PO Box 567, Valdivia, Chile.
authors.forename: Myra Wilson
authors.initials: MW
authors.lastname: Schuster
authors.affiliation: Instituto de Microbiología Clínica, Edificio de Ciencias Biomédicas, Facultad de Medicina, Universidad Austral de Chile, PO Box 567, Valdivia, Chile.
authors.forename: Laura Otth
authors.initials: LO
authors.lastname: Rademacher
authors.affiliation: Instituto de Medicina, Facultad de Medicina, Universidad Austral de Chile, PO Box 567, Valdivia, Chile.
authors.forename: Claudio
authors.initials: C
authors.lastname: Toledo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002677:Chile / Q000453:epidemiology; D005260:Female; D005767:Gastrointestinal Diseases / Q000453:epidemiology / Q000382:microbiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D016481:Helicobacter Infections / Q000382:microbiology; D016480:Helicobacter pylori / Q000235:genetics* / Q000276:immunology*; D006801:Humans; D007983:Lewis Blood Group Antigens / Q000235:genetics / Q000378:metabolism*; D016256:Lewis X Antigen / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009844:Oligosaccharides / Q000235:genetics / Q000378:metabolism
chemical_list: C500822:3-aminopropyl fucopyranosyl-1-2-galactopyranosyl-1-3-(fucopyranosyl-1-4)-2-acetamido-2-deoxyglucopyranosyl-1-3-galactopyranosyl-1-4-glucopyranoside; D007983:Lewis Blood Group Antigens; D016256:Lewis X Antigen; C094162:Lewis Y antigen; C494219:Lewis a oligosaccharide; D008070:Lipopolysaccharides; D009844:Oligosaccharides