"Accelerating aging" chemotherapy on aged animals: protective effect from nutraceutical modulation.
The aim of this study was to test a novel phytocompound in an experimental model of antitumor-induced immunosuppression. Five groups of mice were considered: young (Y) and aged (A) that were given intraperitoneally 10 doses of cyclophosphamide (CPX, 25mg/kg/bw) or CPX plus (150 mg/kg/bw) of the nutraceutical DTS (Denshichi-Tochiu-Sen), and control. After sacrifice, macrophage chemotaxis and serum levels of IFN-gamma, IL-2, and GM-CSF were determined. Liver and urinary bladder were examined histologically, as were the liver and kidney for redox enzymes. CPX significantly decreased macrophage chemotaxis and all cytokines (p < 0.05, A >> Y). DTS restored macrophage function and cytokine concentration (p < 0.001) and partly improved the necro-inflammatory score and substance P receptor expression in the bladder and the redox status in liver and kidney (p < 0.05). Such data suggest that DTS effectively prevents CPX-induced immune suppression and oxidative-inflammatory damage, which are particularly enhanced in aged organisms.
Marotta
Francesco
F
WHO Center for Biotechnology and Traditional Medicine, University of Milano, Italy. fmarchimede@libero.it
Harada
Masatoshi
M
Minelli
Emilio
E
Ono-Nita
Suzanne K
SK
Marandola
Paulo
P
D000375:Aging / Q000187:drug effects*; D000818:Animals; D002633:Chemotaxis / Q000187:drug effects; D003520:Cyclophosphamide / Q000494:pharmacology; D016207:Cytokines / Q000378:metabolism; D019587:Dietary Supplements; D007668:Kidney / Q000187:drug effects / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology*; D018040:Receptors, Neurokinin-1 / Q000378:metabolism; D001743:Urinary Bladder / Q000166:cytology / Q000187:drug effects / Q000378:metabolism
D016207:Cytokines; D020011:Protective Agents; D018040:Receptors, Neurokinin-1; D003520:Cyclophosphamide