Generation of recombinant rotavirus with an antigenic mosaic of cross-reactive neutralization epitopes on VP4.
Recombinant rotavirus (RV) with cDNA-derived chimeric VP4 was generated using recently developed reverse genetics for RV. The rescued virus, KU//rVP4(SA11)-II(DS-1), contains SA11 (simian RV strain, G3P[2])-based VP4, in which a cross-reactive neutralization epitope (amino acids 381 to 401) on VP5* is replaced by the corresponding sequence of a different P-type DS-1 (human RV strain, G2P[4]). Serological analyses with a panel of anti-VP4- and -VP7-neutralizing monoclonal antibodies revealed that the rescued virus carries a novel antigenic mosaic of cross-reactive neutralization epitopes on its VP4 surface. This is the first report of the generation of a recombinant RV with artificial amino acid substitutions.
Komoto
Satoshi
S
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
Kugita
Masanori
M
Sasaki
Jun
J
Taniguchi
Koki
K
D036022:Capsid Proteins / Q000235:genetics*; D003429:Cross Reactions / Q000235:genetics* / Q000276:immunology; D017931:DNA Primers / Q000235:genetics; D000939:Epitopes / Q000235:genetics; D005818:Genetic Engineering; D016297:Mutagenesis, Site-Directed; D009500:Neutralization Tests; D010957:Plasmids / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics*; D011994:Recombinant Proteins / Q000235:genetics* / Q000276:immunology; D012401:Rotavirus / Q000235:genetics*
D036022:Capsid Proteins; D017931:DNA Primers; D000939:Epitopes; D012367:RNA, Viral; D011994:Recombinant Proteins; C060339:VP4 protein, Rotavirus