Typeability of PowerPlex Y (Promega) profiles in selected tissue samples incubated in various environments.
In cases of decomposed bodies, Y chromosomal STR markers may be useful in identification of a male relative. The authors assessed typeability of PowerPlex Y (Promega) loci in post mortem tissue material stored in various environments. Kidney, spleen and pancreas specimens were collected during autopsies of five persons aged 20-30 years, whose time of death was determined within the limit of 14 hours. Tissue material was incubated at 21 degrees C and 4 degrees C in various environmental conditions. DNA was extracted by the organic method from tissue samples collected in 7-day intervals and subsequently typed using the PowerPlexY-STR kit and ABI 310. A fast decrease in the typeability rate was seen in specimens incubated in peat soil and in sand. Kidney tissue samples were typeable in all PowerPlexY-STR loci within 63 days of incubation at 4 degrees C. Faster DNA degradation was recorded in spleen and pancreas specimens. In samples with negative genotyping results, no DNA was found by fluorometric quantitation. Decomposed soft tissues are a potential material for DNA typing.
Niemcunowicz-Janica
Anna
A
Department of Forensic Medicine, Medical University of Bialystok, Poland.
Pepiński
Witold
W
Janica
Jacek Robert
JR
Janica
Jerzy
J
Skawrońska
Małgorzata
M
Koc-Zórawska
Ewa
E
D000328:Adult; D041322:Chromosomes, Human, Y; D016172:DNA Fingerprinting / Q000379:methods*; D005260:Female; D053803:Forensic Genetics / Q000379:methods*; D005554:Forensic Medicine / Q000379:methods; D005828:Genetics, Population; D006801:Humans; D007668:Kidney / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D010179:Pancreas / Q000737:chemistry; D016133:Polymerase Chain Reaction; D013154:Spleen / Q000737:chemistry; D020080:Tandem Repeat Sequences / Q000235:genetics*; D013696:Temperature