title: Isolation and purification of C3 from human plasma.
abstract: The alternative pathway of complement shares its terminal components (C3 and C5 through 9) with the classical pathway, but has several unique components, including factors D, B, and P (properdin). This unit presents methods for assaying total alternative pathway activity and the activity of factors B and D. Radial immunodiffusion (RID) can also be used to measure factor D, B, and P concentrations.
authors.affiliation: University of Louisville, Louisville, Kentucky.
authors.forename: Lynda D
authors.initials: LD
authors.lastname: O'Rear
authors.affiliation: University of Louisville, Louisville, Kentucky.
authors.forename: Gordon D
authors.initials: GD
authors.lastname: Ross
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D003176:Complement C3 / Q000737:chemistry / Q000302:isolation & purification*; D003182:Complement C5 / Q000032:analysis; D015941:Complement Hemolytic Activity Assay / Q000379:methods; D004912:Erythrocytes / Q000276:immunology; D004983:Ethanolamines / Q000737:chemistry; D006801:Humans; D008239:Lysine / Q000737:chemistry; D010949:Plasma / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000737:chemistry
chemical_list: D003176:Complement C3; D003182:Complement C5; D004983:Ethanolamines; D012685:Sepharose; D008239:Lysine; C007369:2-diethylaminoethanol