Determination of antiovarian antibodies after laparoscopic ovarian electrocauterization in patients with polycystic ovary syndrome.
OBJECTIVE
To determine whether ovarian damage consequent to laparoscopic ovarian electrocauterization may result in the development of humoral autoimmunity and production of antiovarian antibodies (AOA).


METHODS
Prospective study.


METHODS
Infertility and gynecologic endoscopy units of one of the medical university hospitals.


METHODS
Sixty-four reproductive-age infertile women (<or=35 y of age) with clomiphene citrate-resistant polycystic ovary syndrome (PCOS) and 50 fertile, healthy, reproductive-aged women (<or=35 y of age) as control group.


METHODS
Laparoscopic ovarian electrocauterization was performed under general anesthesia in clomiphene citrate-resistant PCOS patients for treatment of infertility.


METHODS
Serum samples from 52 patients (before and about 30-40 days after operation) and from 50 fertile healthy controls were assayed for determination of immunoglobulin class-specific AOA (IgM, IgG, and IgA) by using a commercially available ELISA kit that used human ovary as antigen.


RESULTS
A trend of rising in the level of AOA in all isotypes, IgM, IgG, and IgA, was found in sera after operation, compared with the case of sera obtained before operation. But when the ELISA value was calculated according to the formula recommended by the manufacturer, none of the patients were positive for AOA before the surgical intervention, and none of them developed positive AOA after operation. The overall ovulation rate was 81.3%, and the pregnancy rate was 67.2%.


CONCLUSIONS
The ovarian trauma consequent to laparoscopic ovarian electrocauterization in patients with resistant PCOS does not result in a significant production of AOA.
Alborzi
Saeed
S
Department of Obstetrics and Gynecology, Division of Infertility and Gynecologic Endoscopy, Shiraz Institute of Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran. alborzis@sums.ac.ir
Tavazoo
Fahimeh
F
Dehaghani
Alamtaj Samsami
AS
Ghaderi
Abbas
A
Alborzi
Soroosh
S
Alborzi
Mehrnoosh
M
D000328:Adult; D001323:Autoantibodies / Q000032:analysis* / Q000097:blood; D016022:Case-Control Studies; D004564:Electrocoagulation / Q000009:adverse effects / Q000379:methods; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D007247:Infertility, Female / Q000453:epidemiology / Q000209:etiology / Q000276:immunology; D010535:Laparoscopy / Q000009:adverse effects; D008599:Menstruation Disturbances / Q000453:epidemiology; D010053:Ovary / Q000276:immunology* / Q000601:surgery; D011085:Polycystic Ovary Syndrome / Q000097:blood / Q000276:immunology / Q000601:surgery*; D011183:Postoperative Complications / Q000453:epidemiology; D011247:Pregnancy; D016649:Primary Ovarian Insufficiency / Q000453:epidemiology / Q000209:etiology / Q000276:immunology; D055815:Young Adult
D001323:Autoantibodies