Detection of HIV DNA and RNA using PCR.
This extensively updated unit describes the detection of HIV DNA and RNA using PCR using two basic techniques for quantifying the levels of viral DNA and RNA in infected cells. The schemes for both techniques include isolation of nucleic acids, PCR reactions and detection of amplified products using Southern blotting.
Moir
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National Institute of Allergy and Infectious Diseases, NIH, Bethesda, Maryland, USA.
Chun
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D015139:Blotting, Southern / Q000379:methods; D018929:Cell Culture Techniques; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification*; D006678:HIV / Q000235:genetics*; D015658:HIV Infections / Q000821:virology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification*
D004279:DNA, Viral; D012367:RNA, Viral