title: Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase.
abstract: We introduce a method for sequencing peptides by mass spectrometry using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When analyzed by electron transfer dissociation (ETD)-based mass spectrometric sequencing, Lys-N-digested peptides that contain a single lysine residue produce spectra dominated by c-type fragment ions, providing simple ladders for sequence determination. This method should be a valuable strategy for de novo sequencing and the analysis of post-translational modifications.
authors.affiliation: Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
authors.forename: Nadia
authors.initials: N
authors.lastname: Taouatas
authors.forename: Madalina M
authors.initials: MM
authors.lastname: Drugan
authors.forename: Albert J R
authors.initials: AJ
authors.lastname: Heck
authors.forename: Shabaz
authors.initials: S
authors.lastname: Mohammed
mesh_terms: D002460:Cell Line; D006801:Humans; D008239:Lysine / Q000378:metabolism*; D013058:Mass Spectrometry / Q000379:methods; D008666:Metalloendopeptidases / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*
chemical_list: D010455:Peptides; D008666:Metalloendopeptidases; D008239:Lysine