5'-Iodination of solid-phase-linked oligodeoxyribonucleotides.
5'-Iodinated oligodeoxyribonucleotides readily react with 3'-phosphorothioated DNA in the presence of a complementary template to yield a conjugate that is identical to natural DNA in every respect except that one oxygen atom in the phosphodiester backbone is replaced by a sulfur atom. The 5'-iodo group is easily converted to a variety of other functional groups and will quickly react with thiol-containing labels to yield stable thioether conjugates. This unit presents manual and automated procedures for converting the 5'-hydroxyl of protected CPG-bound oligodeoxyribonucleotides to an iodo group and for releasing and purifying the products.
Kool
Eric T
ET
Stanford University, Stanford, California, USA.
Miller
Gregory P
GP
D004247:DNA / Q000737:chemistry; D054879:Halogenation; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010710:Phosphates / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D010710:Phosphates; C083120:phosphorodithioic acid; D004247:DNA