title: Isolation of human eosinophils.
abstract: Highly purified eosinophils can be isolated from peripheral blood by negative selection using anti-CD16 immunomagnetic beads. The basic protocol describes a sequential fractionation of peripheral blood in which eosinophils are separated first from mononuclear cells and then from CD16(+) neutrophils. This technique is easy to use, fast, and highly reproducible. A support protocol describes a staining method that can be used to evaluate the purity of eosinphils.
authors.affiliation: Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.
authors.forename: Kaiser G
authors.initials: KG
authors.lastname: Lim
authors.affiliation: Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.
authors.forename: Peter F
authors.initials: PF
authors.lastname: Weller
mesh_terms: D004804:Eosinophils / Q000166:cytology* / Q000276:immunology; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D017452:Receptors, IgG / Q000737:chemistry / Q000276:immunology
chemical_list: D017452:Receptors, IgG