Isolation of Ly-1+/CD5+ B cells by cell sorting.
Ly-1/CD5 is a 68-kDa glycoprotein that was originally thought to mark the helper subset of T cells. Later it was shown to be present on all T cells and, more recently, on a subset of B cells. Although its function remains the subject of speculation, CD5 expression serves as a useful marker for a functionally distinct population of B cells that has attracted a considerable amount of interest from investigators of both murine and human immune systems. Of critical importance in much of this work is the isolation of pure populations of CD5+ B cells along with appropriate control populations. A flow cytometry technique is presented in this unit, which results in the selection and isolation of two populations of cells from a complex mixture based on physical properties (e.g., size and internal granularity) and correlated expression of several surface molecules.
Hardy
Richard R
RR
Fox Chase Cancer Center, Philadelphia, Pennsylvania, USA.
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000737:chemistry / Q000166:cytology* / Q000276:immunology / Q000378:metabolism; D018956:CD5 Antigens / Q000096:biosynthesis / Q000737:chemistry* / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D051379:Mice; D006377:T-Lymphocytes, Helper-Inducer / Q000166:cytology / Q000276:immunology / Q000378:metabolism
D018956:CD5 Antigens; C526836:Cd5 protein, mouse