Enzymatic synthesis of M(1)G-deoxyribose.
Adducts formed between electrophiles and nucleic acid bases are believed to play a key role in chemically induced mutations and cancer. M(1)G-dR is an endogenous exocyclic DNA adduct formed by the reaction of the dicarbonyl compound malondialdehyde with a dG residue in DNA. It is an intermediate in the synthesis of a class of modified oligodeoxyribonucleotides that are used to study the mutagenicity and repair of M(1)G. This unit presents methods for synthesizing M(1)G-dR by enzymatic coupling.
Schnetz-Boutaud
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NC
Vanderbilt University, Nashville, Tennessee.
Chapeau
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MC
Marnett
L J
LJ
D002384:Catalysis; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D007778:Lactobacillus / Q000201:enzymology; D010430:Pentosyltransferases / Q000378:metabolism*; D013939:Thymidine Phosphorylase / Q000378:metabolism
C089188:3-(2'-deoxyribofuranosyl)pyrimido(1,2-a)purin-10(3H)-one; D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D013939:Thymidine Phosphorylase; C021888:nucleoside deoxyribosyltransferase; D003849:Deoxyguanosine