Plasmacytoid dendritic cells migrate in afferent skin lymph.
Conventional dendritic cells enter lymph nodes by migrating from peripheral tissues via the lymphatic route, whereas plasmacytoid dendritic cells (pDC), also called IFN-producing cells (IPC), are described to gain nodes from blood via the high endothelial venules. We demonstrate here that IPC/pDC migrate in the afferent lymph of two large mammals. In sheep, injection of type A CpG oligodinucleotide (ODN) induced lymph cells to produce type I IFN. Furthermore, low-density lymph cells collected at steady state produced type I IFN after stimulation with type A CpG ODN and enveloped viruses. Sheep lymph IPC were found within a minor B(neg)CD11c(neg) subset expressing CD45RB. They presented a plasmacytoid morphology, expressed high levels of TLR-7, TLR-9, and IFN regulatory factor 7 mRNA, induced IFN-gamma production in allogeneic CD4(pos) T cells, and differentiated into dendritic cell-like cells under viral stimulation, thus fulfilling criteria of bona fide pDC. In mini-pig, a CD4(pos)SIRP(pos) subset in afferent lymph cells, corresponding to pDC homologs, produced type I IFN after type A CpG-ODN triggering. Thus, pDC can link innate and acquired immunity by migrating from tissue to draining node via lymph, similarly to conventional dendritic cells.
Pascale
Florentina
F
Virologie et Immunologie Moléculaires, UR892 Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy-en-Josas, France.
Pascale
Florentia
F
Contreras
Vanessa
V
Bonneau
Michel
M
Courbet
Alexandre
A
Chilmonczyk
Stefan
S
Bevilacqua
Claudia
C
Epardaud
Mathieu
M
Eparaud
Mathieu
M
Niborski
Violeta
V
Riffault
Sabine
S
Balazuc
Anne-Marie
AM
Foulon
Eliane
E
Guzylack-Piriou
Laurence
L
Riteau
Beatrice
B
Hope
Jayne
J
Bertho
Nicolas
N
Charley
Bernard
B
Schwartz-Cornil
Isabelle
I
D000818:Animals; D039521:CD11c Antigen / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D005260:Female; D007113:Immunity, Innate / Q000187:drug effects / Q000502:physiology*; D050839:Interferon Regulatory Factor-7 / Q000276:immunology; D007370:Interferon Type I / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000276:immunology; D008196:Lymph / Q000166:cytology / Q000276:immunology*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D009838:Oligodeoxyribonucleotides / Q000276:immunology / Q000494:pharmacology; D010950:Plasma Cells / Q000166:cytology / Q000276:immunology*; D012756:Sheep; D012867:Skin / Q000166:cytology / Q000276:immunology*; D013552:Swine; D013556:Swine, Miniature; D051199:Toll-Like Receptor 7 / Q000276:immunology; D051217:Toll-Like Receptor 9 / Q000276:immunology / Q000819:agonists; D014780:Viruses / Q000276:immunology
D039521:CD11c Antigen; C526902:CpG ODN 2216; D050839:Interferon Regulatory Factor-7; D007370:Interferon Type I; D009838:Oligodeoxyribonucleotides; D051199:Toll-Like Receptor 7; D051217:Toll-Like Receptor 9; D017493:Leukocyte Common Antigens