title: Overview of affinity tags for protein purification.
abstract: Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the background of total cellular proteins, and for detecting protein-protein interactions. This overview describes the historical basis for the development of affinity tags, affinity tags that are commonly used today, how to choose an appropriate affinity tag for a particular purpose, and several recently developed affinity tag technologies that may prove useful in the near future.
authors.affiliation: Duke University Medical Center, Durham, North Carolina.
authors.forename: Michelle E
authors.initials: ME
authors.lastname: Kimple
authors.affiliation: University of North Carolina Chapel Hill, Chapel Hill, North Carolina.
authors.forename: John
authors.initials: J
authors.lastname: Sondek
mesh_terms: D000345:Affinity Labels; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D000345:Affinity Labels; D011994:Recombinant Proteins