title: Measurement of interleukin-21.
abstract: This unit describes three procedures for measurement of interleukin-21 (IL-21). The first employs the use of an antibody sandwich ELISA. An alternative procedure measures proliferative responses of T cells to a combination of IL-21 and IL-15 using CFSE. Finally, a method to assess IL-21-induced tyrosine phosphorylation of Stat3 in splenic CD8(+) T cells using a flow cytometry-based analysis is described.
authors.affiliation: National Institutes of Health, Bethesda, Maryland.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: National Institutes of Health, Bethesda, Maryland.
authors.forename: Rosanne
authors.initials: R
authors.lastname: Spolski
authors.affiliation: National Institutes of Health, Bethesda, Maryland.
authors.forename: Warren J
authors.initials: WJ
authors.lastname: Leonard
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D045163:Antibodies, Phospho-Specific / Q000276:immunology / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes; D002469:Cell Separation / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry / Q000379:methods; D005452:Fluoresceins / Q000378:metabolism; D007158:Immunologic Techniques; D007159:Immunologic Tests / Q000379:methods; D019409:Interleukin-15 / Q000276:immunology / Q000378:metabolism; D007378:Interleukins / Q000032:analysis* / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation; D051379:Mice; D011994:Recombinant Proteins / Q000276:immunology / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000276:immunology / Q000378:metabolism; D013388:Succinimides / Q000276:immunology / Q000378:metabolism; D000098546:Interleukin-21
chemical_list: C087165:5-(6)-carboxyfluorescein diacetate succinimidyl ester; D000911:Antibodies, Monoclonal; D045163:Antibodies, Phospho-Specific; D005452:Fluoresceins; D019409:Interleukin-15; D007378:Interleukins; D011994:Recombinant Proteins; D050796:STAT3 Transcription Factor; D013388:Succinimides; D000098546:Interleukin-21