Exploiting genetic diversity by directed evolution: molecular breeding of type III polyketide synthases improves productivity.
Applying directed evolution to the phloroglucinol synthase PhlD from Pseudomonas fluorescens Pf-5 has provided the first example of engineering enhanced productivity in a type III polyketide synthase, and a rare instance of improving the activity of a biosynthetic enzyme from secondary metabolism.
Zha
Wenjuan
W
Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801, USA.
Rubin-Pitel
Sheryl B
SB
Zhao
Huimin
H
D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000595:Amino Acid Sequence; D002384:Catalysis; D030562:Databases, Protein; D019020:Directed Molecular Evolution; D014644:Genetic Variation / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D015202:Protein Engineering; D011551:Pseudomonas fluorescens / Q000201:enzymology* / Q000235:genetics*
D000217:Acyltransferases; C020141:flavanone synthetase