Isolation of human eosinophils.
Highly purified eosinophils can be isolated from peripheral blood by negative selection using anti-CD16 immunomagnetic beads. The basic protocol describes a sequential fractionation of peripheral blood in which eosinophils are separated first from mononuclear cells and then from CD16(+) neutrophils. This technique is easy to use, fast, and highly reproducible. A support protocol describes a staining method that can be used to evaluate the purity of eosinphils.
Lim
Kaiser G
KG
Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.
Weller
Peter F
PF
Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts.
D004804:Eosinophils / Q000166:cytology* / Q000276:immunology; D006801:Humans; D018189:Immunomagnetic Separation / Q000379:methods*; D017452:Receptors, IgG / Q000737:chemistry / Q000276:immunology
D017452:Receptors, IgG