DNA synthesis without base protection.
DNA synthesis can be achieved by using O-selective methods for internucleotide bond formation. This greatly simplifies the synthesis of oligodeoxyribonucleotides by eliminating the need for nucleobase protection and deprotection steps. This unit describes strategies that can be used for DNA synthesis without base protection. The discussion includes synthesis of phosphoramidite and H-phosphonate monomers, solid-phase assembly by the phosphoramidite and H-phosphonate methods, and future prospects for DNA synthesis using N-unprotected approaches.
Sekine
Mitsuo
M
Tokyo Institute of Technology, Yokohama, Japan.
D020029:Base Pairing; D004247:DNA / Q000138:chemical synthesis*; D004260:DNA Repair; D003853:Deoxyribonucleosides / Q000138:chemical synthesis / Q000737:chemistry; D008954:Models, Biological; D063065:Organophosphonates / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000737:chemistry; D013698:Templates, Genetic
D003853:Deoxyribonucleosides; D063065:Organophosphonates; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D004247:DNA