title: Hospitalization due to norovirus and genotypes of rotavirus in pediatric patients, state of Espírito Santo.
abstract: Viruses are the leading cause for hospitalization due to gastroenteritis worldwide. Group A rotaviruses (RV) are the most prevalent and are assorted in glycoproteins (G) and protease sensitive (P) dual genotypes based on polymorphic genes that encode the external VP7 and VP4 capsid proteins, respectively. Noroviruses (NoV) have increasingly answered by sporadic gastroenteritis. This study aimed to determine the prevalence of NoV and RV in 68 hospitalized children, between July 2004 and November 2006, at a pediatric hospital in Vitória city, state of Espírito Santo, Southeastern Brazil. Nucleic acid was extracted from fecal suspension following the guanidine-silica procedure. Reverse transcriptase-polymerase chain reaction (RT-PCR) and polyacrylamide gel electrophoresis were employed for NoV and RV detection, respectively. RV genotyping was accomplished using RT-PCR followed by heminested multiplex PCR with specific primers for the most prevalent types of G and P. Fecal samples were positive for NoV and RV in 39.7% (27/68) and 20.5% (14/68), respectively and together were responsible for 60% (41/68) of the cases. RV genotypes were: 50% G9P[8], 28.7% G2P[4], 7.1% G1P[8], G2P[8] and G?P[8]. Vomit was a prominent manifestation observed in 92% and 85% of the NoV and RV cases, respectively. The median hospitalization was 5 and 5.5 days for the patients infected with NoV and RV, respectively. The data showed that NoV prevailed over RV and it also corroborated the emergence of RV G9 genotype followed by G2P[4], reinforcing the need for RV genotype surveillance.
authors.affiliation: Nucleus of Infectious Diseases, Pathology Department, Health Science Center, Federal University of Espírito Santo, Vitória, ES, 29043-900, Brasil.
authors.forename: Leandro Rua
authors.initials: LR
authors.lastname: Ribeiro
authors.forename: Rafael Souto de Oliveira
authors.initials: RS
authors.lastname: Giuberti
authors.forename: Débora Maria Pires Gonçalves
authors.initials: DM
authors.lastname: Barreira
authors.forename: Ketene Wernek
authors.initials: KW
authors.lastname: Saick
authors.forename: José Paulo Gagliardi
authors.initials: JP
authors.lastname: Leite
authors.forename: Marize Pereira
authors.initials: MP
authors.lastname: Miagostovich
authors.forename: Liliana Cruz
authors.initials: LC
authors.lastname: Spano
mesh_terms: D001938:Brazil; D017250:Caliciviridae Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D002648:Child; D002675:Child, Preschool; D018076:DNA, Complementary / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*; D005838:Genotype; D006760:Hospitalization / Q000706:statistics & numerical data; D006801:Humans; D029322:Norovirus / Q000235:genetics* / Q000302:isolation & purification; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000175:diagnosis / Q000453:epidemiology / Q000821:virology*
chemical_list: D018076:DNA, Complementary