In vivo MRI tracking of exogenous monocytes/macrophages targeting brain tumors in a rat model of glioma.
This study has shown that murine monocytes/macrophages (Mo/Ma) can be labeled simply and efficiently with large, green-fluorescent, micrometer-sized particles of iron-oxide (MPIO). Neither size nor proliferation rate of the Mo/Ma is significantly affected by this labeling. The labeled Mo/Ma have been administered intravenously to rats that had developed a glioma following stereotactic injection of C6 cells. The labeled Mo/Ma were shown to target the brain tumors, a process that could be monitored non-invasively using T2*-weighted MRI. MRI observations were confirmed by Prussian blue staining, lectin staining and fluorescence histology. Overall, the results of this study suggest that the use of Mo/Ma may be envisaged in the clinic for vectorizing therapeutic agents toward gliomas.
Valable
Samuel
S
Inserm, U836, Grenoble, France.
Barbier
Emmanuel L
EL
Bernaudin
Myriam
M
Roussel
Simon
S
Segebarth
Christoph
C
Petit
Edwige
E
Rémy
Chantal
C
D000818:Animals; D001932:Brain Neoplasms / Q000473:pathology*; D004195:Disease Models, Animal; D005290:Ferric Compounds; D005910:Glioma / Q000473:pathology*; D007275:Injections, Intravenous; D008264:Macrophages; D008279:Magnetic Resonance Imaging; D008297:Male; D009000:Monocytes; D010316:Particle Size; D051381:Rats; D017208:Rats, Wistar
D005290:Ferric Compounds; C000499:ferric oxide