A mouse model for cytomegalovirus infection.
This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (mCMV). Methods are included for propagating mCMV in cell cultures and for preparing a more virulent form of mCMV from salivary glands of infected mice. A plaque-forming cell (PFC) assay is provided for measuring mCMV titers of infected tissues or virus stocks. In addition, a method is described for preparing the murine embryonic fibroblasts used for propagating mCMV and for the PFC assay.
Brune
W
W
University of Munich, Max von Pettenkofer Institute, Munich, Germany.
Hengel
H
H
Koszinowski
U H
UH
D000818:Animals; D003586:Cytomegalovirus Infections / Q000821:virology; D004195:Disease Models, Animal; D005347:Fibroblasts / Q000821:virology; D006566:Herpesviridae Infections / Q000821:virology; D051379:Mice; D018146:Muromegalovirus / Q000302:isolation & purification / Q000502:physiology; D012469:Salivary Glands / Q000821:virology*; D019562:Viral Load; D010948:Viral Plaque Assay / Q000379:methods*; D014779:Virus Replication