Human Th17 cell clones and natural immune responses.
Immunomodulators such as lipopolysaccharides (LPS) and forskolin change the nature of dendritic cells (DCs) to induce Th1 and Th2 cells, respectively, thereby designated Th1 or Th2 adjuvants. Recent studies showed that Th17-inducing activity can be carried by certain polysaccharides such as beta-glucan derived from Candia albicans. Such activities can be scrutinized by using MLR, cAMP and possibly, differential expression of Notch ligand isoforms. In this review article, we also introduce an effective method to establish human Th17 cell clones and a transcriptome analysis using human Th subpopulations. In vivo relevance to human Th17 responses is discussed.
Matsushita
Sho
S
Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University, Saitama, Japan. shomat@saitama-med.ac.jp
Higashi
Takehiro
T
D000276:Adjuvants, Immunologic / Q000494:pharmacology; D001172:Arthritis, Rheumatoid / Q000276:immunology; D002469:Cell Separation; D002478:Cells, Cultured; D002999:Clone Cells; D018920:Coculture Techniques; D000242:Cyclic AMP / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D007113:Immunity, Innate / Q000235:genetics; D015212:Inflammatory Bowel Diseases / Q000276:immunology; D020381:Interleukin-17 / Q000378:metabolism; D009103:Multiple Sclerosis / Q000276:immunology; D020411:Oligonucleotide Array Sequence Analysis; D011565:Psoriasis / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism; D016176:T-Lymphocyte Subsets / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology; D018418:Th2 Cells / Q000276:immunology
D000276:Adjuvants, Immunologic; D020381:Interleukin-17; D012333:RNA, Messenger; D051880:Receptors, Notch; D000242:Cyclic AMP