title: Variants of parvovirus B19: bioinformatical evaluation of nested PCR assays.
abstract: Variants of parvovirus B19 are currently grouped into three genotypes: 1 (reference B19 strains), 2 and 3. It has been evidenced that isolate K71 of genotype 2 is more prevalent in skin than the conventional B19 genotype 1. In this study we investigated the detection of parvovirus B19 genotypes by using two nested PCRs and evaluating the suitability of these assays by BLAST search of parvovirus isolates. Subsequently, we analyze the present genotypes in skin biopsies. The two nested PCRs employed in this study allow to amplify 41 isolates as confirmed by bioinformatical validation. The molecular epidemiological characterization of our casistics confirmed the presence of isolate K71 in human skin.
authors.affiliation: Department of Public Health and Microbiology, Virology Unit, University of Turin, Turin, Italy.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Bergallo
authors.forename: Cristina
authors.initials: C
authors.lastname: Costa
authors.forename: Francesca
authors.initials: F
authors.lastname: Sidoti
authors.forename: Mauro
authors.initials: M
authors.lastname: Novelli
authors.forename: Renata
authors.initials: R
authors.lastname: Ponti
authors.forename: Carlotta
authors.initials: C
authors.lastname: Castagnoli
authors.forename: Chiara
authors.initials: C
authors.lastname: Merlino
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Bernengo
authors.forename: Rossana
authors.initials: R
authors.lastname: Cavallo
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D019295:Computational Biology / Q000379:methods*; D017931:DNA Primers; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016410:Lymphoma, T-Cell, Cutaneous / Q000821:virology; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000821:virology; D016732:Parvovirus B19, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods*; D012867:Skin / Q000821:virology*
chemical_list: D017931:DNA Primers; D004279:DNA, Viral