Differences in gene expression of granulosa cells from women undergoing controlled ovarian hyperstimulation with either recombinant follicle-stimulating hormone or highly purified human menopausal gonadotropin.
OBJECTIVE
To investigate the differences in the gene expression profile of granulosa cells from preovulatory follicles after controlled ovarian hyperstimulation (COH) with recombinant follicle-stimulating hormone (FSH) or urinary human menopausal gonadotropin (hMG) FSH.


METHODS
Prospective randomized study.


METHODS
University-based facilities for clinical services and research.


METHODS
Thirty women undergoing treatment with vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI).


METHODS
Patients were randomly allocated to receive recombinant FSH or human (hMG) COH. Granulosa cells were collected from follicular fluid after oocyte retrieval, and mRNA were isolated for gene expression analysis.


METHODS
General gene expression profile.


RESULTS
Ninety-six probe sets (85 genes) showed statistically significant differences in expression level in the two groups of granulosa cells. Expression level of luteinizing hormone/human chorionic gonadotropin (LH/hCG) receptor gene and genes involved in biosynthesis of cholesterol and steroids were expressed at lower levels in the hMG-treated cells; inositol 1,4,5-triphosphate-3-kinase-A and S100-calcium-binding-protein-P (anti-apoptosis protein) were expressed at higher levels in hMG than in recombinant FSH.


CONCLUSIONS
The different hormone compositions of the two drugs used for COH had a statistically significant impact on the gene expression profile of preovulatory granulosa cells. Some of these genes may be important for periovulatory events, which suggests that the preparation used for COH is important for granulosa cell function and may influence the developmental competence of the oocyte and the function of corpus luteum.
Grøndahl
Marie Louise
ML
Fertility Clinic, Hvidovre Hospital, Copenhagen University Hospital, Hvidovre, Denmark. marie.groendahl@hvh.regionh.dk
Borup
Rehannah
R
Lee
Young Bae
YB
Myrhøj
Vibeke
V
Meinertz
Helle
H
Sørensen
Steen
S
D000328:Adult; D005260:Female; D005640:Follicle Stimulating Hormone / Q000494:pharmacology*; D020869:Gene Expression Profiling; D006107:Granulosa Cells / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D008596:Menotropins / Q000494:pharmacology*; D020411:Oligonucleotide Array Sequence Analysis; D010062:Ovulation Induction / Q000379:methods*; D011446:Prospective Studies; D011974:Receptors, LH / Q000235:genetics; D011994:Recombinant Proteins / Q000494:pharmacology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D011974:Receptors, LH; D011994:Recombinant Proteins; D042461:Vascular Endothelial Growth Factor A; D008596:Menotropins; D005640:Follicle Stimulating Hormone