Structural and biochemical properties of lipid particles from the yeast Saccharomyces cerevisiae.
The two most prominent neutral lipids of the yeast Saccharomyces cerevisiae, triacylglycerols (TAG) and steryl esters (SE), are synthesized by the two TAG synthases Dga1p and Lro1p and the two SE synthases Are1p and Are2p. In this study, we made use of a set of triple mutants with only one of these acyltransferases active to elucidate the contribution of each single enzyme to lipid particle (LP)/droplet formation. Depending on the remaining acyltransferases, LP from triple mutants contained only TAG or SE, respectively, with specific patterns of fatty acids and sterols. Biophysical investigations, however, revealed that individual neutral lipids strongly affected the internal structure of LP. SE form several ordered shells below the surface phospholipid monolayer of LP, whereas TAG are more or less randomly packed in the center of the LP. We propose that this structural arrangement of neutral lipids in LP may be important for their physiological role especially with respect to mobilization of TAG and SE reserves.
Czabany
Tibor
T
Institute of Biochemistry, Graz University of Technology, Petersgasse 12, A-8010 Graz, Austria.
Wagner
Andrea
A
Zweytick
Dagmar
D
Lohner
Karl
K
Leitner
Erich
E
Ingolic
Elisabeth
E
Daum
Günther
G
D000217:Acyltransferases / Q000378:metabolism; D002152:Calorimetry, Differential Scanning; D015536:Down-Regulation; D005227:Fatty Acids / Q000737:chemistry; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D005838:Genotype; D008055:Lipids / Q000737:chemistry*; D008954:Models, Biological; D009154:Mutation; D010316:Particle Size; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000502:physiology; D013261:Sterols / Q000378:metabolism; D013347:Subcellular Fractions
D005227:Fatty Acids; D008055:Lipids; D029701:Saccharomyces cerevisiae Proteins; D013261:Sterols; D000217:Acyltransferases