Meiosis I is established through division-specific translational control of a cyclin.
In budding yeast, key meiotic events such as DNA replication, recombination, and the meiotic divisions are controlled by Clb cyclin-dependent kinases (Clb-CDKs). Using a novel synchronization procedure, we have characterized the activity of these Clb-CDKs and observed a surprising diversity in their regulation during the meiotic divisions. Clb1-CDK activity is restricted to meiosis I, and Clb3-CDK activity to meiosis II, through 5'UTR-mediated translational control of its transcript. The analysis of cells inappropriately producing Clb3-CDKs during meiosis I furthermore defines Clb3 as an inhibitor of the meiosis I chromosome segregation program. Our results demonstrate an essential role for Clb-CDK regulation in establishing the meiotic chromosome segregation pattern.
Carlile
Thomas M
TM
Center for Cancer Research, Massachusetts Institute of Technology, E17-233, 40 Ames Street, Cambridge, MA 02139, USA.
Amon
Angelika
A
D020121:5' Untranslated Regions / Q000378:metabolism; D002842:Chromatids / Q000378:metabolism; D019926:Cyclin B; D016213:Cyclins / Q000378:metabolism*; D008540:Meiosis; D014176:Protein Biosynthesis; D011494:Protein Kinases / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000166:cytology; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
D020121:5' Untranslated Regions; C068264:CLB1 protein, S cerevisiae; C491083:CLB3 protein, S cerevisiae; D019926:Cyclin B; D016213:Cyclins; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases