title: In situ polymerase chain reaction and hybridization to detect low-abundance nucleic acid targets.
abstract: This unit provides detailed methods and material descriptions for in situ hybridization following in situ amplification of DNA or RNA by PCR. It includes all essential components of the techniques, including variations suitable for different kinds of tissue and cell preparations. Planning, controls, and critical parameters for the amplification steps are discussed.
authors.affiliation: South Carolina Center for Biotechnology, Claflin University, Orangeburg, South Carolina, USA.
authors.forename: Omar
authors.initials: O
authors.lastname: Bagasra
mesh_terms: D003584:Cytological Techniques; D017403:In Situ Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D020401:Primed In Situ Labeling / Q000379:methods