Measurement of mouse and human interleukin 5.
Two fundamentally different types of assays are available for quantitating interleukin 5 in biological samples. One type, a bioassay, is based on the ability of IL-5 to enhance B cell proliferation and immunoglobulin secretion or eosinophil proliferation and differentiation. The other type of assay, an ELISA, uses antibodies against IL-5 to capture and quantitate IL-5 in samples. Advantages and disadvantages of each assay are discussed. The bioassay described in this unit, utilizing BCL(1) cells as the indicator line, has been designed primarily to assay murine IL-5; however, it can also be used to measure human IL-5. The IL-5 ELISA, while sensitive and specific, provides no information about biological activity.
Harriman
G R
GR
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology; D001681:Biological Assay / Q000379:methods*; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D015848:Interleukin-5 / Q000032:analysis* / Q000276:immunology / Q000502:physiology; D051379:Mice; D012680:Sensitivity and Specificity
D007136:Immunoglobulins; D015848:Interleukin-5