Techniques for mammalian cell tissue culture.
This unit opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step-by-step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
Phelan
Mary C
MC
Molecular Pathology Laboratory Network, Maryville, Tennessee, USA.
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D015925:Cryopreservation / Q000379:methods*; D003470:Culture Media; D006801:Humans; D008322:Mammals; D013242:Sterilization / Q000379:methods*
D003470:Culture Media