Overview of affinity tags for protein purification.
Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the background of total cellular proteins, and for detecting protein-protein interactions. This overview describes the historical basis for the development of affinity tags, affinity tags that are commonly used today, how to choose an appropriate affinity tag for a particular purpose, and several recently developed affinity tag technologies that may prove useful in the near future.
Kimple
Michelle E
ME
Duke University Medical Center, Durham, North Carolina.
Sondek
John
J
University of North Carolina Chapel Hill, Chapel Hill, North Carolina.
D000345:Affinity Labels; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification*
D000345:Affinity Labels; D011994:Recombinant Proteins