Culture-Negative intracerebral abscesses in children and adolescents from Streptococcus anginosus group infection: a case series.
We report the use of 16S ribosomal RNA gene amplification and sequencing to diagnose culture-negative intracerebral abscesses in younger patients. These 3 cases demonstrate the optimal application of gene sequencing from direct specimens for patients with negative culture results compromised by antibacterial therapy but histories highly suggestive of acute bacterial infection.
Petti
Cathy A
CA
ARUP Laboratories, University of Utah School of Medicine, Salt Lake City, UT 84108, USA. cathy.petti@aruplab.com
Simmon
Keith E
KE
Bender
Jeffrey
J
Blaschke
Anne
A
Webster
Kathleen A
KA
Conneely
Mark F
MF
Schreckenberger
Paul C
PC
Origitano
Thomas C
TC
Challapalli
Malliswari
M
D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001921:Brain / Q000000981:diagnostic imaging; D001922:Brain Abscess / Q000382:microbiology*; D002648:Child; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011859:Radiography; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000382:microbiology*; D034366:Streptococcus anginosus / Q000235:genetics* / Q000302:isolation & purification
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S