title: Design, synthesis, and amplification of DNA pools for in vitro selection.
abstract: Preparation of a random-sequence DNA pool is presented. The degree of randomization and the length of the random sequence are discussed, as is synthesis of the pool using a DNA synthesizer. Purification of a single-stranded pool and conversion to a double-stranded pool are presented as step-by-step protocols. Support protocols describe determination of the complexity and skewing of the pool, and optimization of amplification conditions.
authors.affiliation: Harvard University, Cambridge, Massachusetts, USA.
authors.forename: J
authors.initials: J
authors.lastname: Pollard
authors.forename: S D
authors.initials: SD
authors.lastname: Bell
authors.forename: A D
authors.initials: AD
authors.lastname: Ellington
mesh_terms: D000097:Acetonitriles; D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009943:Organophosphorus Compounds / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000379:methods*; D052156:SELEX Aptamer Technique / Q000379:methods*
chemical_list: D000097:Acetonitriles; D009943:Organophosphorus Compounds; C434331:phosphoramidite; D004247:DNA; C032159:acetonitrile