title: Free and bound water in normal and cataractous human lenses.
abstract: OBJECTIVE
  To analyze free and total water in human normal and cataractous lenses.
  
  
  METHODS
  Thermogravimetric analysis was used to determine total water, and differential scanning calorimetry was used for free water.
  
  
  RESULTS
  In normal human lenses, the total water content of the nucleus remained unchanged with age, but the state of the water altered. The ratio of free to bound water increased steadily throughout adult life. In a 20-year-old person, there was approximately one bound water molecule for each free water molecule in the lens center, whereas in a 70- to 80-year-old person, there were two free water molecules for each bound water molecule. This conversion of bound to free water does not appear to be simply a consequence of the aggregation of soluble crystallins into high molecular weight aggregates because studies with intact pig lenses, in which such processes were facilitated by heat, did not show similar changes. The region of the lens in which the barrier to diffusion develops at middle age corresponds to a transition zone in which the protein concentration is intermediate between that of the cortex and the nucleus. In cataractous lenses, the free-to-bound water ratio was not significantly different from that of age-matched normal lenses; however, total water content in the center of advanced nuclear cataractous lenses was slightly lower than in normal lenses.
  
  
  CONCLUSIONS
  As the human lens ages, bound water is progressively changed to free water. Advanced nuclear cataract may be associated with lower total hydration of the lens nucleus.
authors.affiliation: Save Sight Institute, Sydney University, Sydney, New South Wales 2001, Australia.
authors.forename: Karl R
authors.initials: KR
authors.lastname: Heys
authors.forename: Michael G
authors.initials: MG
authors.lastname: Friedrich
authors.forename: Roger J W
authors.initials: RJ
authors.lastname: Truscott
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging / Q000502:physiology; D001834:Body Water / Q000378:metabolism*; D002386:Cataract / Q000378:metabolism*; D006801:Humans; D007908:Lens, Crystalline / Q000378:metabolism*; D008875:Middle Aged; D013818:Thermogravimetry