Global changes in and characterization of specific sites of phosphorylation in mouse and human histone H1 Isoforms upon CDK inhibitor treatment using mass spectrometry.
Global changes in the phosphorylation state of human H1 isoforms isolated from UL3 cells have been investigated using mass spectrometry. Relative changes in H1 phosphorylation between untreated cells and cells treated with dexamethasone or various CDK inhibitors were determined. The specific cyclin-dependent kinase consensus sites of phosphorylation on the histone H1 isoforms that show changes in phosphorylation were also investigated. Three sites of phosphorylation on histone H1.4 isoforms have been identified.
Deterding
Leesa J
LJ
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, DHHS, P.O. Box 12233, RTP, North Carolina 27709, USA. deterdi2@niehs.nih.gov
Bunger
Maureen K
MK
Banks
Geoffrey C
GC
Tomer
Kenneth B
KB
Archer
Trevor K
TK
D015075:2-Aminopurine / Q000031:analogs & derivatives / Q000494:pharmacology; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D018844:Cyclin-Dependent Kinases / Q000037:antagonists & inhibitors*; D003907:Dexamethasone / Q000494:pharmacology; D006657:Histones / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D008969:Molecular Sequence Data; D010748:Phosphopeptides / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D010750:Phosphoproteins / Q000032:analysis / Q000276:immunology / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D020033:Protein Isoforms / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011687:Purines / Q000494:pharmacology; D011817:Rabbits; D016415:Sequence Alignment; D012697:Serine Endopeptidases / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014611:Vaccination
D006657:Histones; C469937:N(2)-(2-aminocyclohexyl)-N(6)-(3-chlorophenyl)-9-ethyl-9H-purine-2,6-diamine; D010748:Phosphopeptides; D010750:Phosphoproteins; D020033:Protein Isoforms; D047428:Protein Kinase Inhibitors; D011687:Purines; D015075:2-Aminopurine; D003907:Dexamethasone; D018844:Cyclin-Dependent Kinases; C044499:submandibular proteinase A; D012697:Serine Endopeptidases; C109140:CVT 313