title: Evaluation of a nanotechnology-based carrier for delivery of curcumin in prostate cancer cells.
abstract: We have initiated studies to enhance targeted delivery of an anticancer agent, curcumin, for prostate cancer treatment by incorporating this agent into the liposomes (nanodelivery vehicles primarily composed of phospholipids) coated with prostate membrane specific antigen specific antibodies. We prepared curcumin-loaded liposomes of various lipid compositions by sonication at an average size of 100-150 nm. Un-entrapped curcumin was removed by size exclusion chromatography. Data show that curcumin preferentially partitioned into liposomes prepared from dimyristoyl phosphatidyl choline (DMPC) and cholesterol among the various compositions tested. The anti-proliferative activity of liposomal curcumin was studied using two human prostate cancer cell lines (LNCaP and C4-2B) by a tetrazolium dye-based (MTT) assay. Treatment of cells with liposomal curcumin (5-10 microM) for 24-48 h at 37 degrees C resulted in at least 70-80% inhibition of cellular proliferation without affecting their viability. On the other hand, free curcumin exhibited similar inhibition only at 10-fold higher doses (>50 microM). We also observed that LNCaP cells were relatively more sensitive to liposomal curcumin mediated block of cellular proliferation than C4-2B cells. We are currently developing liposome formulations with targeting ability to further improve the efficacy of curcumin in vivo.
authors.affiliation: Center for Combat Casualty and Life Sustainment Research, Department of Pathology, Uniformed Services University of the Health Sciences (USUHS), Bethesda, MD 20814, USA.
authors.forename: Rajesh L
authors.initials: RL
authors.lastname: Thangapazham
authors.forename: Anu
authors.initials: A
authors.lastname: Puri
authors.forename: Shrikant
authors.initials: S
authors.lastname: Tele
authors.forename: Robert
authors.initials: R
authors.lastname: Blumenthal
authors.forename: Radha K
authors.initials: RK
authors.lastname: Maheshwari
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D003474:Curcumin / Q000737:chemistry / Q000494:pharmacology* / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D004339:Drug Compounding; D006801:Humans; D008081:Liposomes; D008297:Male; D036103:Nanotechnology; D010743:Phospholipids / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000276:immunology; D011471:Prostatic Neoplasms / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000906:Antibodies; D000972:Antineoplastic Agents, Phytogenic; D008081:Liposomes; D010743:Phospholipids; D017430:Prostate-Specific Antigen; D003474:Curcumin