[Study of the subcellular location of the human gene 6 transactivated by nonstructeral protein 5a of hepatitis C virus].
OBJECTIVE
To found the subcellular location of the human gene 6 transactivated by nonstructural protein 5A of hepatitis C virus (NS5ATP6).


METHODS
Green fluorescent protein (GFP) expression vector pEGFP- NS5ATP6 was established. The pEGFP- NS5ATP6 was transfected into HepG2 cells, and analyze the subcellular location of the proteins expressed by NS5ATP6 through Green fluorescent microscopy after 24 hours.


RESULTS
The pEGFP- NS5ATP6 gene was successful cloned, NS5ATP6 can express protein in cells and subcellularly located in cell plasma.


CONCLUSIONS
NS5ATP6 can express protein, and the protein expressed by NS5ATP6 subcellularly located in cell plasma.
Wang
Jian-jun
JJ
The 302 Hospital of PLA, Beijing 100039 China.
Zhao
Ping
P
Jin
Xue-yuan
XY
Cheng
Jun
J
Qing
Song
S
Ding
Ning
N
D045744:Cell Line, Tumor; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D016174:Hepacivirus; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D015533:Transcriptional Activation; D014162:Transfection; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase
D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase