title: Effect of the HP0159 ORF mutation on the lipopolysaccharide structure and colonizing ability of Helicobacter pylori.
abstract: The outer core region of Helicobacter pylori lipopolysaccharide of the majority of isolates contains an alpha-1,6-glucan polymer synthesized by the product of the HP0159 ORF. Structural studies carried out on HP0159 lipopolysaccharide mutants by a combination of chemical methods, mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that insertional inactivation of HP0159 gene in H. pylori strains 26695 and SS1 resulted in formation of a truncated lipopolysaccharide molecule characterized by the presence of a terminal dd-heptose residue in the side-chain outer core fragment and maintaining an inner core backbone structure compared with the wild-type Lewis antigen-expressing strains. Colonization studies with HP0159 mutants of two mouse-colonizing strains, SS1 and M6, confirmed their inability to successfully colonize the murine stomach.
authors.affiliation: Institute for Biological Sciences, National Research Council of Canada, Ottawa, ON, Canada. eleonora.altman@nrc-cnrc.gc.ca
authors.forename: Eleonora
authors.initials: E
authors.lastname: Altman
authors.forename: Vandana
authors.initials: V
authors.lastname: Chandan
authors.forename: Suzon
authors.initials: S
authors.lastname: Larocque
authors.forename: Annie
authors.initials: A
authors.lastname: Aubry
authors.forename: Susan M
authors.initials: SM
authors.lastname: Logan
authors.forename: Evgeny
authors.initials: E
authors.lastname: Vinogradov
authors.forename: Jianjun
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D015169:Colony Count, Microbial; D017353:Gene Deletion; D016480:Helicobacter pylori / Q000737:chemistry / Q000235:genetics* / Q000472:pathogenicity*; D008070:Lipopolysaccharides / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D016254:Mutagenesis, Insertional; D013270:Stomach / Q000382:microbiology; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D008070:Lipopolysaccharides