High performance liquid chromatographic method for the determination of HIV-1 protease inhibitor tipranavir in plasma of patients during highly active antiretroviral therapy.
A new high-performance liquid chromatographic method for the determination of tipranavir in human plasma is described. Quantitative recovery following liquid-liquid-extraction with diethylether from 100 microl of human plasma was achieved. Subsequently, the assay was performed with 67 mM potassium dihydrogen phosphate-acetonitrile as a mobile phase, a Phenomenex C 18 column and UV detection at 255 nm. Linear Standard curves were obtained for concentrations ranging from 2.5 to 400 microg/ml. The calculated intra- and inter-day coefficents of variation were below 7%.
Langmann
Peter
P
Internist-Gastroenterologie, Am Tiefen Weg 2, Karlstadt, Germany. p_langmann@yahoo.de
Heinz
W
W
Klinker
H
H
Schirmer
D
D
Guhl
C
C
Leyh
M
M
Winzer
R
R
D023241:Antiretroviral Therapy, Highly Active; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring; D015658:HIV Infections / Q000097:blood* / Q000188:drug therapy; D017320:HIV Protease Inhibitors / Q000097:blood*; D015497:HIV-1; D006801:Humans; D011725:Pyridines / Q000097:blood*; D011753:Pyrones / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013449:Sulfonamides
D017320:HIV Protease Inhibitors; D011725:Pyridines; D011753:Pyrones; D013449:Sulfonamides; C107201:tipranavir