Serum levels of adipocyte fatty acid-binding protein (AFABP) are increased in chronic haemodialysis (CD).
OBJECTIVE
Adipocyte fatty acid-binding protein (AFABP) was recently introduced as an adipocyte-expressed factor, serum levels of which independently correlate with the development of the metabolic syndrome and cardiovascular disease in humans. In the current study, we investigated renal elimination of this protein by comparing circulating AFABP levels in patients on chronic haemodialysis (CD) with controls. We hypothesized that if renal filtration is a significant route of elimination of AFABP, it would accumulate in CD patients.


METHODS
AFABP was determined by ELISA in control (n = 60) and CD (n = 60) patients and correlated to clinical and biochemical measures of renal function, glucose and lipid metabolism, as well as inflammation, in both groups.


RESULTS
Median serum AFABP levels were more than 10-fold higher in CD patients (510.9 +/- 294.7 microg/l) as compared to controls (44.3 +/- 35.2 microg/l). Furthermore, CD independently predicted AFABP concentrations in multiple regression analysis. In addition, body mass index and free fatty acids were independently associated with circulating AFABP.


CONCLUSIONS
Renal filtration appears as an important route of elimination of AFABP. Future studies should elucidate whether this adipocyte-expressed factor contributes to the increased risk of cardiovascular disease found in end-stage renal disease.
Sommer
Grit
G
Department of Internal Medicine III, University of Leipzig, Leipzig, Germany.
Ziegelmeier
Michaela
M
Bachmann
Anette
A
Kralisch
Susan
S
Lossner
Ulrike
U
Kratzsch
Jürgen
J
Blüher
Matthias
M
Stumvoll
Michael
M
Fasshauer
Mathias
M
D017667:Adipocytes / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D050556:Fatty Acid-Binding Proteins / Q000097:blood*; D005260:Female; D006801:Humans; D007676:Kidney Failure, Chronic / Q000097:blood*; D008297:Male; D008875:Middle Aged; D012044:Regression Analysis; D006435:Renal Dialysis
C493410:FABP4 protein, human; D050556:Fatty Acid-Binding Proteins