Quantitation of DNA and RNA with absorption and fluorescence spectroscopy.
Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers in molecular biology. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques are presented below. These three procedures cover a range from 5 to 10 ng/ml DNA to 50 mg/ml DNA.
Gallagher
S R
SR
Motorola Corporation, Tempe, Arizona, USA.
D000818:Animals; D001562:Benzimidazoles / Q000032:analysis; D004247:DNA / Q000032:analysis*; D004996:Ethidium / Q000032:analysis; D005456:Fluorescent Dyes / Q000032:analysis; D012313:RNA / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D001562:Benzimidazoles; D005456:Fluorescent Dyes; C447680:hoechst 32258; D012313:RNA; D004247:DNA; D004996:Ethidium