Identification of functional domains of phosphoproteins of two morbilliviruses using chimeric proteins.
The paramyxovirus P protein is an essential component of the transcriptase and replicase complex along with L protein. In this article, we have examined the functional roles of different domains of P proteins of two closely related morbilliviruses, Rinderpest virus (RPV) and Peste des petits ruminants virus (PPRV). The PPRV P protein physically interacts with RPV L as well as RPV N protein when expressed in transfected cells, as shown by co-immunoprecipitation. The heterologous L-P complex is biologically active when tested in a RPV minigenome replication/transcription system, only when used with PPRV N protein but not with RPV N protein. Employing chimeric PPRV/RPV cDNAs having different coding regions of P protein in the minigenome replication/transcription system, we identified a region between 290 and 346 aa in RPV P protein necessary for transcription of the minigenome.
Saikia
Paramananda
P
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, India.
Shaila
M S
MS
D000818:Animals; D002460:Cell Line; D006801:Humans; D019590:Nucleocapsid Proteins / Q000235:genetics / Q000378:metabolism; D018107:Peste-des-petits-ruminants virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012302:Rinderpest virus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018411:Spodoptera
D019590:Nucleocapsid Proteins; D010750:Phosphoproteins; D011993:Recombinant Fusion Proteins; D012324:RNA-Dependent RNA Polymerase