A global assembly line for cyanobactins.
More than 100 cyclic peptides harboring heterocyclized residues are known from marine ascidians, sponges and different genera of cyanobacteria. Here, we report an assembly line responsible for the biosynthesis of these diverse peptides, now called cyanobactins, both in symbiotic and free-living cyanobacteria. By comparing five new cyanobactin biosynthetic clusters, we produced the prenylated antitumor preclinical candidate trunkamide in Escherichia coli culture using genetic engineering.
Donia
Mohamed S
MS
Department of Medicinal Chemistry, University of Utah, 30 South 2000 East, Room 201, Salt Lake City, Utah 84112, USA.
Ravel
Jacques
J
Schmidt
Eric W
EW
D000595:Amino Acid Sequence; D016000:Cluster Analysis; D000458:Cyanobacteria / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010456:Peptides, Cyclic / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D013237:Stereoisomerism; D013997:Time Factors
D010456:Peptides, Cyclic