title: Differential function of Tie2 at cell-cell contacts and cell-substratum contacts regulated by angiopoietin-1.
abstract: Tie2 belongs to the receptor tyrosine kinase family and functions as a receptor for Angiopoietin-1 (Ang1). Gene-targeting analyses of either Ang1 or Tie2 in mice reveal a critical role of Ang1-Tie2 signalling in developmental vascular formation. It remains elusive how the Tie2 signalling pathway plays distinct roles in both vascular quiescence and angiogenesis. We demonstrate here that Ang1 bridges Tie2 at cell-cell contacts, resulting in trans-association of Tie2 in the presence of cell-cell contacts. In clear contrast, in isolated cells, extracellular matrix-bound Ang1 locates Tie2 at cell-substratum contacts. Furthermore, Tie2 activated at cell-cell or cell-substratum contacts leads to preferential activation of Akt and Erk, respectively. Microarray analyses and real-time PCR validation clearly show the differential gene expression profile in vascular endothelial cells upon Ang1 stimulation in the presence or absence of cell-cell contacts, implying downstream signalling is dependent upon the spatial localization of Tie2.
authors.affiliation: Department of Structural Analysis, National Cardiovascular Center Research Institute, 5-7-1 Fujishirodai, Suita, Osaka 565-8565, Japan. fuku@ri.ncvc.go.jp
authors.forename: Shigetomo
authors.initials: S
authors.lastname: Fukuhara
authors.forename: Keisuke
authors.initials: K
authors.lastname: Sako
authors.forename: Takashi
authors.initials: T
authors.lastname: Minami
authors.forename: Kazuomi
authors.initials: K
authors.lastname: Noda
authors.forename: Hak Zoo
authors.initials: HZ
authors.lastname: Kim
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Kodama
authors.forename: Masabumi
authors.initials: M
authors.lastname: Shibuya
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Takakura
authors.forename: Gou Young
authors.initials: GY
authors.lastname: Koh
authors.forename: Naoki
authors.initials: N
authors.lastname: Mochizuki
mesh_terms: D042683:Angiopoietin-1 / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D004789:Enzyme Activation; D005109:Extracellular Matrix / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000378:metabolism; D020869:Gene Expression Profiling; D006801:Humans; D007365:Intercellular Junctions / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D052250:Nitric Oxide Synthase Type III / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism; D042787:Receptor, TIE-2 / Q000235:genetics / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D042683:Angiopoietin-1; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; C467200:Foxo1 protein, mouse; D011993:Recombinant Fusion Proteins; D052250:Nitric Oxide Synthase Type III; D042787:Receptor, TIE-2; D051416:Focal Adhesion Protein-Tyrosine Kinases; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases