Generation of HTLV-I-transformed T cell lines.
Viral transformation of T cells is an effective method for obtaining large numbers of T cells that are easily maintained in the laboratory. Human T cell leukemia/lymphoma virus (HTLV-I), a type C retrovirus clearly implicated as the etiologic agent of adult T cell leukemia, can both infect and transform normal T cells. This unit presents a protocol in which human T cells are activated and then transformed with HTLV-I. Detection of virally transformed cells is discussed in the commentary.
Nutman
Thomas B
TB
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
D002461:Cell Line, Transformed; D015368:Human T-lymphotropic virus 1; D006801:Humans; D013601:T-Lymphocytes / Q000166:cytology*