In vitro evaluation of experimental agents for anti-HIV activity.
This unit presents an assay that has proven useful as an initial screening test is an HIV cytopathic effect (CPE) inhibition assay in which immortalized T cell lines (e.g., ATH8 or MT2) that are profoundly sensitive to the cytopathic effect of certain strains of HIV are utilized as target cells. Additional protocols assess the anti-HIV activity of certain candidate agents by measuring inhibition of syncytium formation or p24 gag protein production by ELISA. Calculation of the 50% inhibitory concentration (IC(50)) is also presented.
Richman
D D
DD
University of California, San Diego, La Jolla, California, USA.
Johnson
V A
VA
Mayers
D M
DM
Shirasaka
T
T
O'Brien
M C
MC
Mitsuya
H
H
D019380:Anti-HIV Agents / Q000494:pharmacology*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000821:virology*; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D006678:HIV / Q000502:physiology*; D006801:Humans; D007158:Immunologic Techniques
D019380:Anti-HIV Agents