title: [Expression of pS2, TGF-alpha and PCNA in the gastric mucosa of young rats with endotoxemia].
abstract: OBJECTIVE
  Growth, regeneration and reparation of gastric mucosal epithelium may relate to the expression of peptides. This study aimed to investigate the effect of pS2, TGF-alpha and PCNA in endotoxin-induced acute gastric mucosal injury in young rats.
  
  
  METHODS
  Eighteen-day-old Wistar rats were randomly injected intraperitoneally with lipopolysaccharide (LPS) (5 mg/kg) or normal saline (control). The gastric mucosal specimens were harvested 1.5, 3, 6, 24, 48, and 72 hrs after LPS or normal saline injection (n=8 each). The pathological changes of the gastric mucosa were observed by hematoxylin-eosin staining. The expression of pS2,TGF-alpha and PCNA was measured by immunohistochemistry SP method.
  
  
  RESULTS
  Gastric mucosal injuries were the most serious 6 hrs after LPS injection, characterized by massive erosion, bleeding and cord necrosis of the gastric mucosa paralleling with gastric longitudinal axis. PCNA expression in the gastric mucosa in the LPS group 3, 6, 24 and 48 hrs after LPS injection was significantly lower than that in the control group (P<0.01). pS2 expression in the gastric mucosa weakened 1.5 hrs after LPS injection, recovered to the control level at 3 hrs and was significantly higher than the control at 6, 24, 48 and 72 hrs of LPS injection (P<0.01). TGF-alpha expression in the gastric mucosa in the LPS group increased significantly 6, 24 and 48 hrs after LPS injection when compared with the control group (P<0.01).
  
  
  CONCLUSIONS
  PCNA expression may be associated with the proliferation activity of the gastric mucosa in the process of gastric mucosal injury/reparation. pS2 and TGF-alpha might participate in the defense and reparation of gastric mucosal cells through mediating cell proliferation following acute gastric mucosal injury.
authors.affiliation: Department of Pediatrics, 463 Hospital of Chinese People's Liberation Army, Shenyang 110042, China.
authors.forename: Chun-Ying
authors.initials: CY
authors.lastname: Liu
authors.forename: Li-Jie
authors.initials: LJ
authors.lastname: Wang
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Wang
authors.forename: Qing-Jie
authors.initials: QJ
authors.lastname: Lu
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Jiang
authors.forename: Mei
authors.initials: M
authors.lastname: Sun
mesh_terms: D000818:Animals; D019446:Endotoxemia / Q000378:metabolism*; D005260:Female; D005753:Gastric Mucosa / Q000737:chemistry* / Q000473:pathology; D007150:Immunohistochemistry; D008297:Male; D010455:Peptides / Q000032:analysis*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis*; D051381:Rats; D017208:Rats, Wistar; D016211:Transforming Growth Factor alpha / Q000032:analysis*; D000071164:Trefoil Factor-2
chemical_list: D010455:Peptides; D018809:Proliferating Cell Nuclear Antigen; D016211:Transforming Growth Factor alpha; D000071164:Trefoil Factor-2