title: Flow cytometry of bacterial membrane potential and permeability.
abstract: This chapter describes reliable flow cytometric methods for assessment of two important physiologic characteristics of bacteria, membrane potential and membrane permeability, which can provide indications of the effects of antimicrobial agents on microorganisms.
authors.affiliation: The Center for Microbial Cytometry and Howard M Shapiro, West Newton, MA, USA.
authors.forename: Howard M
authors.initials: HM
authors.lastname: Shapiro
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena / Q000187:drug effects; D002232:Carbocyanines / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects*; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000502:physiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D002232:Carbocyanines; D005456:Fluorescent Dyes; C098830:TO-PRO-3; C081554:3,3'-diethyloxacarbocyanine