title: Evaluation of reference tissue model and tissue ratio method for 5-HTT using [(123)I] ADAM tracer.
abstract: The serotonin (5-hydroxytryptamine, or 5-HT) transporters (5-HTT) are target-sites for commonly used antidepressants. [(123)I] ADAM is a novel radiotracer that selectively binds the 5-HTT of the central nervous system. The aim for this study was to compare four-parameter model (FPM) with three-parameter model (TPM) from non-invasive reference tissue model (RTM) for 5-HTT quantification using the cerebellum as indirect input function. Furthermore, we compared tracer kinetic model with the tissue ratio (TR) method. The binding potential (BP) values derived from both models were almost the same, but ratio of delivery (R(1)) in TPM had smaller standard deviation than the FPM. There was also significant correlation between BP and specific uptake ratio (SUR). In conclusion, simplified reference tissue model (SRTM) was the better choice because of its stability and convenient implementation for non-invasive quantification of brain SPECT studies. The correlation found between BP and SUR supports the use of TR method for quantification of 5-HTT to avoid arterial sampling in dynamic SPECT scan.
authors.affiliation: Department of Biomedical Imaging and Radiological Sciences, National Yang-Ming University, No. 155, Section 2, Li-Nong Road, Baitou, Taipei City 112, Taiwan, ROC.
authors.forename: Bang-Hung
authors.initials: BH
authors.lastname: Yang
authors.forename: Shyh-Jen
authors.initials: SJ
authors.lastname: Wang
authors.forename: Yuan-Hwa
authors.initials: YH
authors.lastname: Chou
authors.forename: Tung-Ping
authors.initials: TP
authors.lastname: Su
authors.forename: Shih-Pei
authors.initials: SP
authors.lastname: Chen
authors.forename: Jih-Shian
authors.initials: JS
authors.lastname: Lee
authors.forename: Jyh-Cheng
authors.initials: JC
authors.lastname: Chen
mesh_terms: D000328:Adult; D000465:Algorithms; D002531:Cerebellum / Q000000981:diagnostic imaging / Q000378:metabolism; D002928:Cinanserin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D005260:Female; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008954:Models, Biological; D011485:Protein Binding; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D050486:Serotonin Plasma Membrane Transport Proteins / Q000378:metabolism*; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon; D055815:Young Adult
chemical_list: C413491:2-((2-((dimethylamino)methyl)phenyl)thio)-5-iodophenylamine; D019275:Radiopharmaceuticals; C493230:SLC6A4 protein, human; D050486:Serotonin Plasma Membrane Transport Proteins; D002928:Cinanserin