Mechanism of calcium hydroxide-induced neutrophil migration into air-pouch cavity.
The aim of this study was to investigate cellular migration induced by calcium hydroxide to air-pouch cavities in mice. The migration was more specific to neutrophil and was dose and time dependent (peaking 96 h after stimulation). This migration was inhibited by pretreatment with thalidomide, indomethacin, MK886, meloxicam, dexamethasone, MK886 associated with indomethacin, and MK886 associated with indomethacin and dexamethasone. The air-pouch exudate from animals stimulated with calcium hydroxide showed an increase of leukotriene-B(4) (LTB(4)), interleukin-1beta, tumor necrosis factor alpha (TNF-alpha), cytokine-induced neutrophil chemoattractant (KC), and macrophage inflammatory protein 2 (MIP-2) release. Pretreatment with 3% thioglycollate increased the macrophage population in the air pouch but did not change neutrophil migration. Depleting the resident mast cells through chronic pretreatment with compound 48/80 did not alter neutrophil migration in response to calcium hydroxide. It was possible to conclude that calcium hydroxide-induced neutrophil migration to the air-pouch cavity in mice is mediated by LTB(4), TNF-alpha, KC, MIP-2, and prostaglandins, but it was not dependent on macrophages or mast cells.
Costa
Mariana Machado Teixeira de Moraes
MM
Department of Pediatric Dentistry, School of Dentistry, Sao Paulo State University, Araçatuba, Brazil. moraes_mari@hotmail.com <moraes_mari@hotmail.com>
de Oliveira
Sandra Helena Penha
SH
Gomes-Filho
João Eduardo
JE
D000388:Air; D000818:Animals; D002126:Calcium Hydroxide / Q000009:adverse effects*; D002634:Chemotaxis, Leukocyte / Q000502:physiology*; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D020556:Neutrophil Infiltration / Q000502:physiology*; D011453:Prostaglandins / Q000378:metabolism; D012387:Root Canal Filling Materials / Q000009:adverse effects*; D040521:Subcutaneous Tissue
D016207:Cytokines; D011453:Prostaglandins; D012387:Root Canal Filling Materials; D002126:Calcium Hydroxide