Zymography of metalloproteinases.
Zymography is an electrophoretic technique enabling visualization of the number and approximate size of peptidases in a sample on the basis of their hydrolysis of a protein substrate within the gel. The technique is particularly useful for analyzing the peptidase composition of complex biological samples because visualization depends directly on proteolytic activity. This unit presents a representative zymography protocol for the study of matrix metallopeptidases (MMPs).
Troeberg
Linda
L
Imperial College London, London, United Kingdom.
Nagase
Hideaki
H
Imperial College London, London, United Kingdom.
D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D006868:Hydrolysis; D020782:Matrix Metalloproteinases / Q000737:chemistry*; D008970:Molecular Weight; D013379:Substrate Specificity
D020782:Matrix Metalloproteinases