One-step enzymatic purification of PCR products for direct sequencing.
The polymerase chain reaction (PCR) has become a powerful and widespread method for analyzing DNA. This protocol offers convenient enzymatic purification of PCR products for direct sequencing without the need for further subcloning, precipitation, or column purification. All reactions may be performed in 96-well PCR plates and are compatible with large-scale sequencing. This method is designed to be convenient and rapid. It is suitable for a variety of PCR templates, including plasmid, lambda, and genomic DNA.
Kim
J B
JB
Harvard Medical School, Boston, Massachusetts, USA.
Blackshaw
S
S
D000469:Alkaline Phosphatase; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005090:Exodeoxyribonucleases; D005826:Genetics, Medical; D005827:Genetics, Microbial / Q000379:methods; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA / Q000379:methods*
D004247:DNA; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I; D000469:Alkaline Phosphatase