title: Heterologous expression of two ferulic acid esterases from Penicillium funiculosum.
abstract: Two recombinant ferulic acid esterases from Penicillium funiculosum produced in Aspergillus awamori were evaluated for their ability to improve the digestibility of pretreated corn stover. The genes, faeA and faeB, were cloned from P. funiculosum and expressed in A. awamori using their native signal sequences. Both enzymes contain a catalytic domain connected to a family 1 carbohydrate-binding module by a threonine-rich linker peptide. Interestingly, the carbohydrate binding-module is N-terminal in FaeA and C-terminal in FaeB. The enzymes were purified to homogeneity using column chromatography, and their thermal stability was characterized by differential scanning microcalorimetry. We evaluated both enzymes for their potential to enhance the cellulolytic activity of purified Trichoderma reesei Cel7A on pretreated corn stover.
authors.affiliation: National Bioenergy Center, National Renewable Energy Laboratory, Golden, CO 80401, USA. eric_knoshaug@nrel.gov
authors.forename: Eric P
authors.initials: EP
authors.lastname: Knoshaug
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Selig
authors.forename: John O
authors.initials: JO
authors.lastname: Baker
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Decker
authors.forename: Michael E
authors.initials: ME
authors.lastname: Himmel
authors.forename: William S
authors.initials: WS
authors.lastname: Adney
mesh_terms: D000327:Adsorption; D001230:Aspergillus / Q000235:genetics* / Q000378:metabolism*; D001665:Binding Sites; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000737:chemistry; D010407:Penicillium / Q000145:classification / Q000235:genetics* / Q000378:metabolism*; D011485:Protein Binding; D015202:Protein Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D013045:Species Specificity; D013379:Substrate Specificity
chemical_list: D004800:Enzymes, Immobilized; D011994:Recombinant Proteins; D002482:Cellulose; D002265:Carboxylic Ester Hydrolases; C070196:feruloyl esterase