Peptide array-based screening of human mesenchymal stem cell-adhesive peptides derived from fibronectin type III domain.
Human mesenchymal stem cell-adhesive peptides were screened based on the amino acid sequence of fibronectin type III domain 8-11 (FN-III(8-11)) using a peptide array synthesized by the Fmoc-chemistry. Using hexameric peptide library of FN-III(8-11) scan, we identified the ALNGR (Ala-Leu-Asn-Gly-Arg) peptide that induced cell adhesion as well as RGDS (Arg-Gly-Asp-Ser) peptide. After incubation for 2h, approximately 68% of inoculated cells adhere to the ALNGR peptide disk. Adhesion inhibition assay with integrin antibodies showed that the ALNGR peptide interacts with integrin beta1 but not with alphavbeta3, indicating that the receptors for ALNGR are different from RGDS. Additionally, the ALNGR peptide expressed cell specificities for adhesion: cell adhesion was promoted for fibroblasts but not for keratinocytes or endotherial cells. The ALNGR peptide induced cell adhesion and promoted cell proliferation without changing its property. It is therefore useful for the construction of functional biomaterials.
Okochi
Mina
M
Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Nomura
Shigeyuki
S
Kaga
Chiaki
C
Honda
Hiroyuki
H
D000595:Amino Acid Sequence; D001681:Biological Assay; D002448:Cell Adhesion / Q000187:drug effects; D015815:Cell Adhesion Molecules / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D002460:Cell Line; D005353:Fibronectins / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000737:chemistry* / Q000378:metabolism; D008969:Molecular Sequence Data; D009842:Oligopeptides / Q000302:isolation & purification / Q000494:pharmacology; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D040081:Protein Array Analysis / Q000379:methods*; D017434:Protein Structure, Tertiary; D016596:Vinculin / Q000378:metabolism
D015815:Cell Adhesion Molecules; D005353:Fibronectins; D009842:Oligopeptides; D019151:Peptide Library; D010455:Peptides; C501364:fibronectin type III like peptide, human; D016596:Vinculin; C046617:arginyl-glycyl-aspartyl-serine