Effect of histone deacetylase inhibitor on proliferation of biliary tract cancer cell lines.
OBJECTIVE
To explore the effect of histone deacetylase inhibitor, trichostatin A (TSA) on the growth of biliary tract cancer cell lines (gallbladder carcinoma cell line and cholangiocarcinoma cell line) in vivo and in vitro, and to investigate the perspective of histone deacetylase inhibitor in its clinical application.


METHODS
The survival rates of gallbladder carcinoma cell line (Mz-ChA-l cell line) and cholangiocarcinoma cell lines (QBC939, KMBC and OZ cell lines) treated with various doses of TSA were detected by methylthiazoy tetrazolium (MTT) assay. A nude mouse model of transplanted gallbladder carcinoma (Mz-ChA-l cell line) was successfully established, and changes in the growth of transplanted tumor after treated with TSA were measured.


RESULTS
TSA could inhibit the proliferation of gallbladder carcinoma cell line (Mz-ChA-l cell line) and cholangiocarcinoma cell lines (QBC939, KMBC and OZ cell lines) in a dose-dependent manner. After the nude mouse model of transplanted gallbladder carcinoma (Mz-ChA-l cell line) was successfully established, the growth of cancer was inhibited in the model after treated with TSA.


CONCLUSIONS
TSA can inhibit the growth of cholangiocarcinoma and gallbladder carcinoma cell lines in vitro and in vivo.
Xu
Li-Ning
LN
Institute of Hepatobiliary Surgery of PLA General Hospital, Beijing 100853, China. chaoyue528@sohu.com
Wang
Xin
X
Zou
Sheng-Quan
SQ
D000818:Animals; D001650:Bile Duct Neoplasms / Q000401:mortality / Q000473:pathology*; D001653:Bile Ducts, Intrahepatic; D002455:Cell Division / Q000187:drug effects*; D045744:Cell Line, Tumor; D018281:Cholangiocarcinoma / Q000401:mortality / Q000473:pathology*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005706:Gallbladder Neoplasms / Q000401:mortality / Q000473:pathology*; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009368:Neoplasm Transplantation; D015996:Survival Rate; D014183:Transplantation, Heterologous
D004791:Enzyme Inhibitors; D056572:Histone Deacetylase Inhibitors; D006877:Hydroxamic Acids; C012589:trichostatin A