Probing protein structure and dynamics by hydrogen exchange-mass spectrometry.
Hydrogen exchange rates at peptide backbone amide linkages of proteins are highly sensitive to changes in protein structure and dynamics. Hydrogen exchange-mass spectrometry (HX-MS) can be used to measure hydrogen/deuterium exchange in protein fragments and allows the study of large proteins with high sensitivity and at very low concentrations as well as determination of the intermolecular distribution of deuterium. This unit provides a brief discussion of the interplay between protein dynamics and hydrogen exchange, a detailed prescription for designing and performing typical HX-MS experiments, and common procedures for processing and interpreting HX-MS data.
Wang
Lintao
L
University of Nebraska, Lincoln, Nebraska.
Smith
David L
DL
University of Nebraska, Lincoln, Nebraska.
D003903:Deuterium / Q000737:chemistry / Q000378:metabolism; D041961:Deuterium Exchange Measurement / Q000379:methods*; D006859:Hydrogen / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D013058:Mass Spectrometry / Q000379:methods*; D010446:Peptide Fragments / Q000032:analysis; D011487:Protein Conformation; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D013696:Temperature
D010446:Peptide Fragments; D011506:Proteins; D006859:Hydrogen; D003903:Deuterium