Changes of RAPD profile of Trypanosoma cruzi II with Canova and Benznidazole.
Chagas disease, caused by the protozoan Trypanosoma cruzi, involves immunomediated processes. Canova (CA) is a homeopathic treatment indicated in the diseases in which the immune system is depressed. This study evaluated the Random Amplification of Polymorphic DNA (RAPD) profile of T. cruzi under the influence of CA and Benznidazole (BZ). Mice infected with the genetic lineage of T. cruzi II (Y strain) were divided into 4 groups: Infected animals treated with saline solution (control group); treated with CA; treated with BZ; treated with CA and BZ combined. Treatment was given at the 5th-25th days of infection (D5-25). The parasites were isolated by haemoculture in Liver Infusion Tryptose (LIT) medium: at D5 (before treatment), D13, 15 and 25 (during treatment) and D55 and 295 (after treatment). DNA was extracted from the mass of parasites. RAPD was done with the primers lambdagt11-F, M13F-40 and L15996, the amplified products were eletrophoresed through a 4% polyacrylamide gel. Data were analyzed by the coefficient of similarity using the DNA-POP program. 163 markers were identified, 5 of them monomorphic. CA did not act against the parasites when used alone. The RAPD profiles of parasites treated with BZ and CA+BZ were different from those in the control group and in the group treated with CA. The actions of the CA and BZ were different and the action of BZ was different from the action of CA+BZ. These data suggest that CA may interact with BZ. The differences in the RAPD profile of the Y strain of T. cruzi produced by BZ, CA+BZ and the natural course of the infection suggest selection/suppression of populations.
Aleixo
Denise Lessa
DL
Universidade Estadual de Maringá, Maringá, Paraná, Brazil. denisealeixo@hotmail.com
Ferraz
Fabiana Nabarro
FN
de Melo
Carolina Sundin
CS
Gomes
Mônica Lúcia
ML
Toledo
Max Jean
MJ
Kaneshima
Edílson Noboyoshi
EN
Bersani-Amado
Ciomar Aparecida
CA
Araújo
Silvana Marques
SM
D000818:Animals; D014355:Chagas Disease / Q000188:drug therapy / Q000469:parasitology; D003435:Crotalid Venoms / Q000494:pharmacology*; D016054:DNA, Protozoan / Q000032:analysis*; D008419:Materia Medica / Q000494:pharmacology; D051379:Mice; D009593:Nitroimidazoles / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D019105:Random Amplified Polymorphic DNA Technique; D014344:Trypanocidal Agents / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000302:isolation & purification
C513575:Canova medication; D003435:Crotalid Venoms; D016054:DNA, Protozoan; D008419:Materia Medica; D009593:Nitroimidazoles; D010936:Plant Extracts; D014344:Trypanocidal Agents; C009999:benzonidazole