Enzymic thin film coatings for bioactive materials.
Bioactive materials have been explored for a broad range of applications including biocatalysts, biosensors, antifouling membranes and other functional and smart materials. We report herein a unique method for preparation of bioactive materials through a spin coating process. Specifically, we investigated the preparation of protease Subtilisin Carlsberg-coated plastic films and examined their activities for hydrolysis of chicken egg albumin (CEA). The process generated enzymic coatings with a typical loading of 13 microg/cm2, retaining 46% of the enzyme activity for hydrolysis of CEA in aqueous solutions. Interestingly, the surface-coated protease thin film not only catalyzed the hydrolysis of CEA in aqueous solutions, but also showed good activity for solid-state CEA that was coated on top of the enzyme thin film.
Tong
Xiaodong
X
Biotechnology Institute and Department of Bioproducts and Biosystems Engineering, University of Minnesota, St. Paul, Minnesota 55108, USA.
Trivedi
Archana
A
Jia
Hongfei
H
Zhang
Minjuan
M
Wang
Ping
P
D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000737:chemistry; D006868:Hydrolysis; D008422:Materials Testing; D008567:Membranes, Artificial; D010969:Plastics / Q000737:chemistry*; D020860:Subtilisin / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D010969:Plastics; D020860:Subtilisin