title: Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients.
abstract: OBJECTIVE
  To investigate DNA fragmentation by using terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling in relation to World Health Organization parameters and computer-aided sperm analysis (CASA) in sperm to determine the possibility of obtaining a correlation among CASA parameters, sperm morphology, and DNA fragmentation.
  
  
  METHODS
  Sperm analysis according to World Health Organization parameters, terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) for sperm DNA fragmentation, and CASA for sperm movement. Prospective study.
  
  
  METHODS
  All the patients were under clinical management, consulting for hypofertility at a fertility center in France.
  
  
  METHODS
  One thousand six hundred thirty-three men who were referred for infertility investigation, including a complete sperm analysis.
  
  
  METHODS
  Sperm analysis and DNA damage testing.
  
  
  METHODS
  Sperm morphology, DNA fragmentation, and movement characteristics.
  
  
  RESULTS
  One third of the patients had a TUNEL rate of >30%. Analysis of the 21 semen parameters tested revealed that 7 of them were significantly correlated with the TUNEL results.
  
  
  CONCLUSIONS
  World Health Organization sperm parameters and DNA damage are complementary, rather than strongly linked. This should be considered to more fully understand the paternal contribution in assisted reproductive technologies failures.
authors.affiliation: Laboratoire d'Eylau, Paris, France. pcb@eylau.fr
authors.forename: Paul
authors.initials: P
authors.lastname: Cohen-Bacrie
authors.forename: Stephanie
authors.initials: S
authors.lastname: Belloc
authors.forename: Yves J R
authors.initials: YJ
authors.lastname: Ménézo
authors.forename: Patrice
authors.initials: P
authors.lastname: Clement
authors.forename: Jamal
authors.initials: J
authors.lastname: Hamidi
authors.forename: Moncef
authors.initials: M
authors.lastname: Benkhalifa
mesh_terms: D000328:Adult; D004249:DNA Damage; D053938:DNA Fragmentation; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology*