title: Use of polyurethane foam and 3-hydroxy-7,8-benzo-1,2,3,4-tetrahydroquinoline for determination of nitrite by diffuse reflectance spectroscopy and colorimetry.
abstract: Polyurethane foam (PUF) has been suggested as a solid polymeric reagent for determination of nitrite. The determination is based on the diazotization of end toluidine groups of PUF with nitrite in acidic medium followed by coupling of polymeric diazonium cation with 3-hydroxy-7,8-benzo-1,2,3,4-tetrahydroquinoline. The intensely colored polymeric azodye formed in this reaction can be used as a convenient analytic form for the determination of nitrite by diffuse reflectance spectroscopy (c (min) = 0.7 ng mL(-1)). The possibility of using a desktop scanner, digital camera, and computer data processing for the numerical evaluation of the color intensity of the polymeric azodye has been investigated. A scanner and digital camera can be used for determination of nitrite with the same sensitivity and reproducibility as with diffuse reflectance spectroscopy. The approach developed was applied for determination of nitrite in river water and human exhaled breath condensate.
authors.affiliation: Chemistry Department, Moscow State University, Moscow, GSP-2, 119992, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Apyari
authors.forename: S G
authors.initials: SG
authors.lastname: Dmitrienko
authors.forename: V M
authors.initials: VM
authors.lastname: Ostrovskaya
authors.forename: E K
authors.initials: EK
authors.lastname: Anaev
authors.forename: Y A
authors.initials: YA
authors.lastname: Zolotov
mesh_terms: D000393:Air Pollutants / Q000032:analysis* / Q000737:chemistry; D001391:Azo Compounds / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D003124:Colorimetry / Q000379:methods; D004396:Coloring Agents / Q000737:chemistry; D006801:Humans; D009573:Nitrites / Q000032:analysis*; D011140:Polyurethanes / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods; D044005:Tetrahydroisoquinolines / Q000737:chemistry*; D014052:Toluidines / Q000737:chemistry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D000393:Air Pollutants; D001391:Azo Compounds; D004396:Coloring Agents; D009573:Nitrites; D011140:Polyurethanes; D044005:Tetrahydroisoquinolines; D014052:Toluidines; D014874:Water Pollutants, Chemical; C028279:polyurethane foam