title: Serologic and genetic characterization analyses of a highly pathogenic influenza virus (H5N1) isolated from an infected man in Shenzhen.
abstract: Highly pathogenic avian influenza (HPAI) H5N1 virus caused a wave of outbreaks in China during 2005--2006, resulting in a total of 20 cases of human infection in 14 provinces of China. On June16, 2006, a case of H5N1 human infection was confirmed in Shenzhen. The virus isolated from the patient, A/Guangdong/2/06, was characterized genetically and the relationship between the tracheal virus load and the antibody titer of the infected man was analyzed. Serological analysis confirmed that the patient's neutralizating antibodies had been generated 2 weeks after the onset of symptoms. The patient's serum antibodies could efficiently neutralize A/Guandong/2/06 infectivity in vitro. Phylogenetic analysis showed that the H5N1 virus of Shenzhen belonged to subclade 2.3.4, which contained viruses that were mainly responsible for the outbreaks in domestic poultry and in the cases of human infection in southern China. Homology and molecular characterization analysis revealed that all the segments of Shenzhen H5N1 virus still belonged to avian segments. Several specific amino acid residue mutations were detected.
authors.affiliation: Centers for Disease Control and Prevention, Shenzhen, Guangdong, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Cheng
authors.forename: Chunli
authors.initials: C
authors.lastname: Wu
authors.forename: Jianfan
authors.initials: J
authors.lastname: He
authors.forename: Xing
authors.initials: X
authors.lastname: Lv
authors.forename: Shunxiang
authors.initials: S
authors.lastname: Zhang
authors.forename: Li
authors.initials: L
authors.lastname: Zhou
authors.forename: Jingwen
authors.initials: J
authors.lastname: Wang
authors.forename: Riqiang
authors.initials: R
authors.lastname: Deng
authors.forename: Qingxing
authors.initials: Q
authors.lastname: Long
authors.forename: Xunzhang
authors.initials: X
authors.lastname: Wang
authors.forename: Jinquan
authors.initials: J
authors.lastname: Cheng
mesh_terms: D000328:Adult; D000595:Amino Acid Sequence; D000914:Antibodies, Viral / Q000097:blood*; D002681:China; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification / Q000235:genetics* / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity; D007251:Influenza, Human / Q000821:virology*; D008297:Male; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010802:Phylogeny; D016415:Sequence Alignment; D012689:Sequence Homology, Nucleic Acid; D014132:Trachea / Q000821:virology; D019562:Viral Load; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D000914:Antibodies, Viral; D014764:Viral Proteins