title: [The presence and polymorphism of genes encoding serine protease autotransporters (spate) among the Escherichia coli isolated in the region of Gdansk from children with diarrhea].
abstract: The group of 96 strains ofEscherichia coli isolated from children with diarrhea were investigated towards the presence and polymorphism of genes encoding autotransporters that belong to the group of proteins named SPATE (Serine Protease Autotransporters ofEnterobacteriaceae). Based on the results of restriction analysis of the products of PCR reaction 8 different types of genes encoding SPATE were detected. It was found that 39 strains contained one gene of SPATE, 15 strains contained two different genes and 3 different genes were detected in the case of 3 strains. The analysis of combination of presence of genes encoding SPATE let us divide the investigated group of strains into 17 different genotypes. The analysis of polymorphism of genes encoding SPATE seems to be very promising tool for exploring the genetic diversity among pathogenic E. coli.
authors.affiliation: Katedra Chemii, Technologii i Biotechnologii Zywności, Wydział Chemiczny, Politechnika Gdańska.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Ziomek
authors.forename: Piotr
authors.initials: P
authors.lastname: Szweda
authors.forename: Julianna
authors.initials: J
authors.lastname: Kurlenda
authors.forename: Rafał
authors.initials: R
authors.lastname: Kochanowski
authors.forename: Grazyna
authors.initials: G
authors.lastname: Sikorska-Wiśniewska
mesh_terms: D002352:Carrier Proteins / Q000235:genetics* / Q000378:metabolism; D002648:Child; D003967:Diarrhea / Q000382:microbiology*; D004926:Escherichia coli / Q000145:classification / Q000201:enzymology*; D004927:Escherichia coli Infections / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010802:Phylogeny; D011044:Poland; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D037521:Virulence Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; D029968:Escherichia coli Proteins; D037521:Virulence Factors; D012697:Serine Endopeptidases