title: [The effect of gossypol, praziquantel and artemether on recombinant lactate dehydrogenase from Schistosoma japonicum].
abstract: OBJECTIVE
  To detect the effect of gossypol, praziquantel and artemether on activity of the recombinant lactate dehydrogenase of Schistosoma japonicum (rSjLDH).
  
  
  METHODS
  Effect of gossypol (0-0.10 mmol/L), praziquantel (0-0.20mmol/L) and artemether (0-0.10 mmol/L) on the enzymatic activity of rSjLDH was detected by spectrophotometric method in standard reaction system established before, same solvent of each reagent was used as control. Results were analyzed by SPSS13.0 software.
  
  
  RESULTS
  Compared with the control, considerable inhibition for both reduction and oxidation reactions catalyzed by rSjLDH was observed at 0.04 mmol/L of gossypol (P < 0.01). The enzyme activities of rSjLDH for reduction and oxidation reactions were inhibited by 91.3% and 89.1% respectively at 0.1 mmol/L of gossypol, com-pared with the control (P < 0.01). To praziquantel, enzymatic activity inhibition was observed at 0.02 mmol/L for oxidation reaction and at 0.06 mmol/L for reduction reaction (P < 0.05). At 0.2 mmol/L of praziquantel, enzymatic activities were inhibited by 83.8% for reduction reaction and 72.2% for oxidation reaction respectively, than that of the control (P < 0.01). No inhibition for both reduction and oxidation reactions was observed at 0.1 mmol/L of artemether, compared with that of the control (P > 0.05).
  
  
  CONCLUSIONS
  Gossypol and praziquantel show a high inhibition on rSjLDH. SiLDH may be one of the molecular targets of praziquantel.
authors.affiliation: Department of Parasitology, Hainan Medical College, Haikou 571101, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Lv
authors.forename: Xu-chu
authors.initials: XC
authors.lastname: Hu
authors.forename: Can
authors.initials: C
authors.lastname: Huang
authors.forename: Hong-yan
authors.initials: HY
authors.lastname: Xie
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.forename: Shou-yi
authors.initials: SY
authors.lastname: Chen
authors.forename: Zhong-dao
authors.initials: ZD
authors.lastname: Wu
authors.forename: Xin-bing
authors.initials: XB
authors.lastname: Yu
mesh_terms: D000818:Animals; D000077549:Artemether; D037621:Artemisinins / Q000494:pharmacology*; D002384:Catalysis / Q000187:drug effects; D006072:Gossypol / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D011223:Praziquantel / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000378:metabolism; D012549:Schistosoma japonicum / Q000201:enzymology*; D013053:Spectrophotometry
chemical_list: D037621:Artemisinins; D011994:Recombinant Proteins; D011223:Praziquantel; D000077549:Artemether; D007770:L-Lactate Dehydrogenase; D006072:Gossypol