Production of recombinant adeno-associated viral vectors and use for in vitro and in vivo administration.
Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors. Two detailed methods are provided for viral vector injection into rat brain and for histological detection of transgene expression, including lacZ and GFP.
Choi
Vivian W
VW
University of North Carolina, Chapel Hill, North Carolina, USA.
Asokan
Aravind
A
Haberman
Rebecca A
RA
McCown
Thomas J
TJ
Samulski
Richard Jude
RJ
D000818:Animals; D000229:Dependovirus / Q000502:physiology*; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D018014:Gene Transfer Techniques / Q000295:instrumentation; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D049452:Green Fluorescent Proteins; D001616:beta-Galactosidase