Measurement of mouse and human interleukin 10.
Interleukin 10 (IL-10) is a multifunctional cytokine produced by T cells and other cell types. It regulates activities of a variety of the cells of the immune system and may play a crucial role in cross-regulation of different types of T cells. Two methods of quantitating IL-10 are provided in this unit. The first is an enzyme-linked immunosorbent assay (ELISA) using a pair of monoclonal antibodies specific for mouse IL-10. This method has the advantages of high specificity and insensitivity to other cytokines present in test samples. The second method is a bioassay which has the advantages that both human and mouse IL-10 are detected and that functional protein is measured. Both methods have similar sensitivity.
Mosmann
Tim
T
University of Alberta, Edmonton, Alberta.
D000818:Animals; D000911:Antibodies, Monoclonal; D001681:Biological Assay / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis; D016753:Interleukin-10 / Q000032:analysis* / Q000276:immunology; D051379:Mice; D012680:Sensitivity and Specificity; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D016753:Interleukin-10; D007371:Interferon-gamma