Detection and isolation of cytokine secreting cells using the cytometric cytokine secretion assay.
The cell-surface affinity matrix technology, otherwise called "secretion assay" or "capture assay," represents a new, innovative method for the analysis and enrichment of viable cells according to secreted molecules such as antibodies or cytokineof T cells to induce cytokine secretion is included. Alternate protocols provide very rapid and simple procedures for the direct detection of antigen-specific T cells from small numbers of PBMCs or whole blood without magnetic enrichment.
Assenmacher
Mario
M
Miltenyi Biotec, Bergisch-Gladbach, Germany.
Löhning
Max
M
Deutsches Rheumaforschungszentrum Berlin, Berlin, Germany.
Radbruch
Andreas
A
Deutsches Rheumaforschungszentrum Berlin, Berlin, Germany.
D000818:Animals; D000941:Antigens / Q000276:immunology; D001681:Biological Assay / Q000379:methods*; D016207:Cytokines / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D000941:Antigens; D016207:Cytokines