title: Expression of inflammatory-related factors in porcine anterior pituitary-derived cell line.
abstract: Recent studies have shown that undifferentiated stem cells act as immunomodulators. To investigate the immunomodulatory function of the progenitor cells of the anterior pituitary gland, we attempted to establish a stem/progenitor cell line from the porcine anterior pituitary gland, and to detail its inflammatory cytokine expression. A cloned cell line from the porcine anterior pituitary gland was established and was designated as the porcine anterior pituitary-derived cell line (PAPC). PAPC expressed the mRNA of Nanog and Oct-4, and showed positive immunoreactivity for beta-catenin and Hes1 in its nucleus. PAPC grew stably by repeated passage and rapidly in the EGF and bFGF containing medium. RT-PCR showed that PAPC expressed mRNA of IL-1alpha, IL-6, IL-12, IL-15, IL-18 and TLR4. PAPC expressed S100alpha and IL-18 protein, which was localized in the marginal epithelial cells of Rathke's pouch. These results suggest that PAPC is a stem/progenitor cell and may regulate anterior pituitary cell function through an immuno-endocrine pathway.
authors.affiliation: Laboratory of Functional Morphology, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, 981-8555 Sendai, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nagai
authors.forename: Hideki
authors.initials: H
authors.lastname: Ogasawara
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Taketa
authors.forename: Hisashi
authors.initials: H
authors.lastname: Aso
authors.forename: Takashi
authors.initials: T
authors.lastname: Kanaya
authors.forename: Masato
authors.initials: M
authors.lastname: Miyake
authors.forename: Kouichi
authors.initials: K
authors.lastname: Watanabe
authors.forename: Shyuichi
authors.initials: S
authors.lastname: Ohwada
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Muneta
authors.forename: Takahiro
authors.initials: T
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D007249:Inflammation / Q000276:immunology / Q000378:metabolism; D010903:Pituitary Gland, Anterior / Q000166:cytology* / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013234:Stem Cells / Q000166:cytology* / Q000276:immunology*; D013552:Swine / Q000378:metabolism*
chemical_list: D016207:Cytokines; D012333:RNA, Messenger