A simple method for isolating disomic strains of Saccharomyces cerevisiae.
A simple method to select disomic (N + 1) strains that should be applicable for almost any chromosome in Saccharomyces cerevisiae is presented. A diploid heterozygous for a KanMX knock-out mutation in an essential gene is sporulated and viable geneticin (G418)-resistant colonies selected. Disomic products of a missegregation or non-disjunction event containing a copy of both the wild-type essential gene and its complementary KanMX knock-out allele make up most of the viable colonies. This method has been used to isolate disomic haploids for a variety of chromosomes. It is appropriately named MARV (for missegregation-associated restoration of viability) and is easily adaptable to virtually any strain.
Zebrowski
David C
DC
Department of Microbiology and Molecular Genetics, UMDNJ-Graduate School of Biomedical Sciences, Newark, NJ 07101-1709, USA.
Kaback
David B
DB
D015825:Chromosomes, Fungal / Q000235:genetics*; D004171:Diploidy; D005839:Gentamicins / Q000494:pharmacology; D006238:Haploidy; D016254:Mutagenesis, Insertional / Q000379:methods*; D016366:Open Reading Frames; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics* / Q000502:physiology; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics; D013172:Spores, Fungal / Q000254:growth & development; D014170:Transformation, Genetic
D005839:Gentamicins; D029701:Saccharomyces cerevisiae Proteins; C010680:antibiotic G 418