title: A quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain.
abstract: Inhibition of terminal respiratory oxidases by nitric oxide (NO) plays important physiological roles in signaling and host defense. Using a bacterial quinol oxidase and mitochondrial cytochrome c oxidase, this chapter describes simple polarographic methods to quantify the kinetic characteristics of inhibition by NO. This chapter points out the inherent pitfalls of both experimental design and data analysis and compares alternative methods. Additionally, it describes a system designed to acquire polarographic and spectral data simultaneously to permit identification of spectral intermediates under defined conditions.
authors.affiliation: Department of Biological Sciences, University of Essex, Colchester, United Kingdom.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Mason
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Holladay
authors.forename: Peter
authors.initials: P
authors.lastname: Nicholls
authors.forename: Mark
authors.initials: M
authors.lastname: Shepherd
authors.forename: Chris E
authors.initials: CE
authors.lastname: Cooper
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D004579:Electron Transport / Q000187:drug effects*; D004789:Enzyme Activation / Q000187:drug effects; D020128:Inhibitory Concentration 50; D008954:Models, Biological; D009569:Nitric Oxide / Q000493:pharmacokinetics / Q000494:pharmacology*; D020030:Nitric Oxide Donors / Q000032:analysis / Q000494:pharmacology; D010088:Oxidoreductases / Q000037:antagonists & inhibitors*; D010100:Oxygen / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D020030:Nitric Oxide Donors; D009569:Nitric Oxide; D010088:Oxidoreductases; D010100:Oxygen