Screening of a Saccharomyces cerevisiae nonessential gene deletion collection for altered susceptibility to a killer peptide.
Approximately 4,800 Saccharomyces cerevisiae mutants deleted for nonessential genes were screened for alterations in susceptibility to a synthetic killer peptide (KP). None of the tested strains, including mutants resistant to conventional antifungal drugs, showed increased or decreased susceptibility to KP in comparison with the parental strain. The results may reflect the peculiar mechanism of action of KP and claim the possible avoidance of vital resistant mutants.
Conti
Stefania
S
Dipartimento di Patologia e Medicina di Laboratorio, Sezione di Microbiologia, University of Parma, Parma, Italy.
Magliani
Walter
W
Giovati
Laura
L
Libri
Irene
I
Maffei
Domenico Leonardo
DL
Salati
Antonella
A
Polonelli
Luciano
L
D017353:Gene Deletion; D055646:Killer Factors, Yeast; D008826:Microbial Sensitivity Tests; D009154:Mutation; D009183:Mycotoxins / Q000737:chemistry*; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism
C089359:K1 killer toxin; D055646:Killer Factors, Yeast; D009183:Mycotoxins; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins