Participation of nitric oxide in the color change induced by UV radiation in the crab Chasmagnathus granulatus.
The ability of UV radiation to stimulate color change in vertebrates is well known; however, the signaling pathway involved is not fully explained. Since nitric oxide (NO) is among the candidates for this role, in this study the participation of NO signaling in the pigment migration induced by UV radiation in melanophores of the crab Chasmagnathus granulatus was investigated. When the NO donor, SIN-1, was incubated with pieces of epidermis, there was an induction of a dose-dependent pigment dispersion (in vitro assays). When male adults were exposed to different doses of UVA and UVB, N(G)-nitro-l-arginine-methyl-ester, an NO synthase (NOS) blocker produced a decrease of the pigment dispersion induced by UV (in vivo assays). However, in similar assays, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide, an NO scavenger, decreased only the pigment dispersion induced by UVA. Interestingly, buthionine sulfoximine did not produce any change in pigment dispersion induced by UVA (in vivo assays) and SIN-1 (in vitro assays). Our results using NADPH-diaphorase histochemistry and immunocytochemistry against nNOS indicated the production of NO by epidermal cells. In conclusion, we suggest that NO is a key molecule for the induction of pigment dispersion in the melanophores of Chasmagnthus granulatus, and also that NOS activation is a fundamental step for this process.
Vargas
Marcelo Alves
MA
Fundação Universidade Federal do Rio Grande - FURG, Departamento de Ciências Fisiológicas, Programa de Pós-graduação em Ciências Fisiológicas - Fisiologia Animal Comparada, Rio Grande, RS, Brazil.
Cruz
Bruno Pinto
BP
Maciel
Fábio Everton
FE
Geihs
Marcio Alberto
MA
Cousin
João Carlos Brahn
JC
Trindade
Gilma Santos
GS
Baisch
Ana Luiza Muccillo
AL
Allodi
Silvana
S
Nery
Luiz Eduardo Maia
LE
D000818:Animals; D003386:Brachyura / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects*; D001938:Brazil; D004305:Dose-Response Relationship, Drug; D008297:Male; D008547:Melanophores / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D008981:Molsidomine / Q000031:analogs & derivatives / Q000494:pharmacology; D009569:Nitric Oxide / Q000502:physiology*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D010858:Pigmentation / Q000187:drug effects / Q000528:radiation effects*; D014466:Ultraviolet Rays
D020030:Nitric Oxide Donors; D009569:Nitric Oxide; C002385:linsidomine; D008981:Molsidomine