title: Metabolism of prebiotic products containing beta(2-1) fructan mixtures by two Lactobacillus strains.
abstract: The capacity of two probiotic strains, isolated from human breast milk, to use several beta(2-1) fructan mixtures as carbon and energy source in in vitro cultures has been tested. Results showed that both strains, Lactobacillus gasseri CECT5714 and Lactobacillus fermentum CECT5716, reached higher growth levels on culture media containing fructooligosaccharide mixtures produced by enzymatic synthesis, compared to those obtained by inulin hydrolysis. Furthermore, the shortest beta(2-1) fructan, kestose, was the only prebiotic compound in the mixtures significantly metabolized in all growth media tested. Analysis of short-chain fatty acid production showed no correlation between the fatty acid profile produced and the carbon source used in each experiment. These data could serve to select appropriate beta(2-1) fructans to be used as prebiotics for L. gasseri CECT5714 and L. fermentum CECT5716 and to design suitable symbiotic food products containing the mentioned lactobacilli.
authors.affiliation: Department of Biotechnology, Puleva Biotech S.A., Camino de Purchil 66, 18004 Granada, Spain. obanuelos@pulevabiotech.es
authors.forename: Oscar
authors.initials: O
authors.lastname: Bañuelos
authors.forename: Layla
authors.initials: L
authors.lastname: Fernández
authors.forename: José M
authors.initials: JM
authors.lastname: Corral
authors.forename: Magdalena
authors.initials: M
authors.lastname: Valdivieso-Ugarte
authors.forename: José L
authors.initials: JL
authors.lastname: Adrio
authors.forename: Javier
authors.initials: J
authors.lastname: Velasco
mesh_terms: D003470:Culture Media; D005260:Female; D005630:Fructans / Q000378:metabolism*; D006801:Humans; D007778:Lactobacillus / Q000145:classification / Q000254:growth & development / Q000378:metabolism*; D052197:Limosilactobacillus fermentum / Q000254:growth & development / Q000378:metabolism*; D009844:Oligosaccharides / Q000378:metabolism; D019936:Probiotics
chemical_list: D003470:Culture Media; D005630:Fructans; D009844:Oligosaccharides; C120489:oligofructose