Growth and manipulation of yeast.
This unit describes preparation of selected media for growing yeast and also discusses strain storage and revival. Protocols are provided for the assay of beta-galactosidase in liquid culture and for transformation using lithium acetate.
Treco
D A
DA
Massachusetts General Hospital, Boston, Massachusetts, USA.
Reynolds
A
A
Lundblad
V
V
D000085:Acetates; D003470:Culture Media; D046508:Culture Techniques / Q000379:methods*; D012441:Saccharomyces cerevisiae / Q000254:growth & development*; D014170:Transformation, Genetic; D001616:beta-Galactosidase
D000085:Acetates; D003470:Culture Media; D001616:beta-Galactosidase; C488804:lithium acetate