Experimental autoimmune encephalomyelitis in the rat.
This unit details the materials and methods required for both active induction and adoptive transfer of experimental autoimmune encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains.
Swanborg
Robert H
RH
Wayne State University School of Medicine, Detroit, Michigan.
Stepaniak
Jolie A
JA
Wayne State University School of Medicine, Detroit, Michigan.
D000818:Animals; D004198:Disease Susceptibility; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology*; D006168:Guinea Pigs; D004676:Myelin Basic Protein / Q000276:immunology; D051381:Rats; D011919:Rats, Inbred Strains; D013045:Species Specificity; D013601:T-Lymphocytes / Q000276:immunology
D004676:Myelin Basic Protein