title: Synthesis of a polyrotaxane-based macroporous polymer as stationary phase for capillary electrochromatography via host-guest complexation of N,N '-ethylenedianilinediacrylamide with statistically methylated beta-cyclodextrin.
abstract: A synthetic route to acrylamide-based monolithic stationary phases for CEC with rotaxane-type immobilized derivatized beta-CD was explored. N,N'-Ethylenedianilinediacrylamide was synthesized as the water-insoluble crosslinker forming water-soluble inclusion complexes with statistically methylated beta-CD. Mixed-mode stationary phases were synthesized by free radical copolymerization of the bisacrylamide-CD host-guest complex with water-soluble monomers and an additional water-soluble crosslinker in aqueous solution. Complex formation in solution and inclusion of the pseudorotaxane into the polymeric network (formation of a polyrotaxane architecture) were studied by means of (1)H-NMR chemical shift analysis, CD modified micellar EKC (CD-MEKC), 2D-NOESY spectroscopy, and solid state( 13)C-NMR spectroscopy. The presence of a mixed-mode selectivity of the stationary phase based on hydrophobic and hydrophilic interaction was confirmed by CEC with neutral polar and nonpolar solutes.
authors.affiliation: Department of Natural Sciences, University of Kassel, Kassel, Germany.
authors.forename: Annika
authors.initials: A
authors.lastname: Wahl
authors.forename: Fuad
authors.initials: F
authors.lastname: Al-Rimawi
authors.forename: Ingo
authors.initials: I
authors.lastname: Schnell
authors.forename: Olga
authors.initials: O
authors.lastname: Kornysova
authors.forename: Audrius
authors.initials: A
authors.lastname: Maruska
authors.forename: Ute
authors.initials: U
authors.lastname: Pyell