Measurement of human and mouse interleukin-12.
This unit describes functional assays for measurement of bioactive IL-12 and ELISAs for measurement of IL-12 protein. The functional assays are based on the ability of IL-12 to stimulate proliferation of PHA-activated T lymphoblasts ("PHA blasts"). The ELISAs are technically simpler to perform than the functional assays, but cannot distinguish bioactive from inactive cytokine.
Gately
Maurice K
MK
Hoffmann-La Roche Inc, Nutley, New Jersey.
Chizzonite
Richard
R
Hoffmann-La Roche Inc, Nutley, New Jersey.
Presky
David H
DH
Hoffmann-La Roche Inc, Nutley, New Jersey.
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D049109:Cell Proliferation; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D018664:Interleukin-12 / Q000032:analysis* / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D010835:Phytohemagglutinins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
D010835:Phytohemagglutinins; D018664:Interleukin-12