title: Concurrent peste des petits ruminants virus and pestivirus infection in stillborn twin lambs.
abstract: Concurrent infection with peste des petits ruminants virus (PPRV) and pestivirus was diagnosed in stillborn twin lambs. With the flock history, the findings of epidermal syncytial cells and necrotizing bronchitis/bronchiolitis prompted testing for PPRV infection, and PPRV antigen was detected by immunohistochemistry (IHC) in the skin, lungs, kidneys, rumen, and thymus. Macroscopic anomalies that were typical of border disease included scoliosis, brachygnathism, prognathism, arthrogryposis, hydranencephaly, cerebellar hypoplasia, and hairy fleece; pestiviral antigen was detected by IHC in the brain, liver, lungs, and kidneys. Tissues from both lambs were positive by reverse transcriptase-polymerase chain reaction (RT-PCR) for PPRV and pestivirus. To the authors' knowledge, PPR has not been reported previously as a congenital infection or in combination with pestiviral infection.
authors.affiliation: Faculty of Veterinary Medicine, Department of Pathology, Kirikkale University, Yahsihan, Kirikkale, Turkey. oguzkul72@yahoo.com
authors.forename: O
authors.initials: O
authors.lastname: Kul
authors.forename: N
authors.initials: N
authors.lastname: Kabakci
authors.forename: A
authors.initials: A
authors.lastname: Ozkul
authors.forename: H
authors.initials: H
authors.lastname: Kalender
authors.forename: H T
authors.initials: HT
authors.lastname: Atmaca
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D017809:Fatal Outcome; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D029021:Peste-des-Petits-Ruminants / Q000151:congenital / Q000821:virology*; D018107:Peste-des-petits-ruminants virus / Q000235:genetics / Q000302:isolation & purification*; D010576:Pestivirus / Q000235:genetics / Q000302:isolation & purification*; D018182:Pestivirus Infections / Q000151:congenital / Q000662:veterinary* / Q000821:virology; D011247:Pregnancy; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000821:virology*
chemical_list: D012367:RNA, Viral