Analysis of trinucleotide repeats in myotonic dystrophy.
Myotonic dystrophy is a genetic disorder characterized in 99% of clinically diagnosed families by an unstable CTG repeat in the 3-untranslated region of a gene encoding a serine-threonine protein kinase. There is no one method to detect the entire range of expansion sizes possible in affected patients, so current diagnostic approaches rely on analyzing samples by hybridization of both polymerase chain reaction (PCR)-amplified CTG repeats (CTG-PCR) and genomic DNA. In this unit, the the Basic Protocol 1 describes the analysis of PCR-amplified repeats transferred to a nylon membrane by Southern blotting and hybridized to an alkaline phosphatase-labeled probe. The first support protocol describes a vacuum blotting technique for rapid transfer of the PCR product to the nylon membrane and the second support protocol describes the use of a radiolabeled oligonucleotide probe for hybridization. Analysis of genomic DNA by similar hybridization techniques is outlined in the second basic protocol. Myotonic dystrophy is a genetic disorder characterized in 99% of clinically diagnosed families by an unstable CTG repeat Myotonic dystrophy is a genetic disorder characterized in 99% of clinically diagnosed families by an unstable CTG repeat.
Surh
L C
LC
Children's Hospital of Eastern Ontario, Ottawa, Ontario, Canada.
Mahadevan
M
M
Korneluk
R G
RG
D015139:Blotting, Southern; D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005826:Genetics, Medical; D006801:Humans; D008297:Male; D009223:Myotonic Dystrophy / Q000235:genetics*; D010761:Phosphorus Radioisotopes; D016133:Polymerase Chain Reaction / Q000379:methods; D018911:Trinucleotide Repeats; D014618:Vacuum
D010761:Phosphorus Radioisotopes; D004247:DNA