title: PPE protein (Rv3425) from DNA segment RD11 of Mycobacterium tuberculosis: a novel immunodominant antigen of Mycobacterium tuberculosis induces humoral and cellular immune responses in mice.
abstract: Subtractive DNA hybridization of pathogenic M. bovis and BCG, and comparative genome-wide DNA microarray analysis of M. tuberculosis H37Rv and BCG identified several RD, designated as RD1 to RD16, between M. tuberculosis and M. bovis on the one hand and BCG on the other. These regions cover 108 ORF of M. tuberculosis H37Rv, and are deleted from all 13 BCG sub-strains currently used as anti-tuberculosis vaccines in different parts of the world. In this study, we evaluated cellular and humoral immune response in C57BL/6 mice immunized with the PPE protein Rv3425, encoded by an ORF found in RD11 of M. tuberculosis. Rv3425 protein induced an increased Th1/Th2 type immune response in mice, characterized by an elevated concentration of IFN-gamma in antigen stimulated splenocyte culture and a strong IgG(1) antibody response. These results provide evidence on the immunogenicity of the PPE protein Rv3425 which, together with its reported immunodominant characteristics, imply that it may be a candidate for development of a vaccine for the control of TB.
authors.affiliation: State Key Laboratory of Genetic Engineering, Institute of Genetics, Fudan University, Shanghai 200433, China.
authors.forename: Jiuling
authors.initials: J
authors.lastname: Wang
authors.forename: Yaqing
authors.initials: Y
authors.lastname: Qie
authors.forename: Hongmei
authors.initials: H
authors.lastname: Zhang
authors.forename: Bingdong
authors.initials: B
authors.lastname: Zhu
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Jiazhen
authors.initials: J
authors.lastname: Chen
authors.forename: Honghai
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D000917:Antibody Formation; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D007111:Immunity, Cellular; D016056:Immunodominant Epitopes / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000276:immunology; D010446:Peptide Fragments / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology; D032581:Tuberculosis Vaccines
chemical_list: D000942:Antigens, Bacterial; D016056:Immunodominant Epitopes; D010446:Peptide Fragments; D032581:Tuberculosis Vaccines