[A modified dye test for Toxoplasma gondii infection].
A modified dye test with microplate was to be established to detect Toxoplasma antibodies with cell-cultured Toxoplasma gondii. Numbers of stained and unstained tachyzoites were estimated in every 100 tachyzoites in each well after dyeing with methylene blue. The dilution with 50% tachyzoites stained was used as final dilution. Better results of the microplate dye test has been received when the concentration of tachyzoites in suspension reaches 10(9)/ml with 1% sodium citrate as accessory factor.
Xu
Hui
H
Department of Clinical Laboratory, Meitan General Hospital, Beijing 100028, China. huixu2004@tom.com
Ni
An-Ping
AP
Cui
Qing-Tao
QT
Hao
Ying
Y
D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D006801:Humans; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D009500:Neutralization Tests; D012680:Sensitivity and Specificity; D013194:Staining and Labeling / Q000379:methods; D014122:Toxoplasma / Q000276:immunology*; D014123:Toxoplasmosis / Q000097:blood / Q000175:diagnosis* / Q000469:parasitology
D000913:Antibodies, Protozoan; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M