Determination of sialic acids released from glycoproteins using capillary zone electrophoresis/electrospray ionization mass spectrometry.
A simple method for the determination of the two most abundant sialic acids released from glycoproteins based on CZE-MS is presented. Several parameters like BGE with various organic modifiers and sheath liquids were studied with respect to their suitability for the fast and easy analysis of the selected compounds by CZE-MS. Finally, a BGE containing 10 mM ammonium acetate allowed the quantification of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) in glycoproteins as well as human plasma samples. LOD and LOQ were 2 microg/mL and 6 microg/mL, respectively.
Ortner
Karin
K
Institute of Analytical Chemistry, Johannes-Kepler-University, Linz, Austria.
Buchberger
Wolfgang
W
D019075:Electrophoresis, Capillary / Q000379:methods*; D006023:Glycoproteins / Q000097:blood / Q000737:chemistry*; D006801:Humans; D019158:N-Acetylneuraminic Acid / Q000032:analysis; D009438:Neuraminic Acids / Q000032:analysis; D012794:Sialic Acids / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D006023:Glycoproteins; D009438:Neuraminic Acids; D012794:Sialic Acids; C032592:N-glycolylneuraminic acid; D019158:N-Acetylneuraminic Acid