title: Cultivation of Trypanosoma cruzi epimastigotes in low glucose axenic media shifts its competence to differentiate at metacyclic trypomastigotes.
abstract: This study offers an insight into why Trypanosoma cruzi epimastigotes lose their capacity to differentiate into metacyclic forms, if maintained in culture media long-term through serial passages. The biological and metabolic behaviour of two T. cruzi strains isolated from various origins (human, opossum), and maintained under two schedules (alternate triatomine/mouse passages and serial culture media) were compared. To determine the effect of the environment on the parasites, the epimastigotes were grown under extreme conditions (high and low glucose concentrations), and the glucose consumption, ammonia production and changes in pH, either in one compartment (along the growth curve) or two compartments (induced metacyclogenesis) were compared. The glucose effect on the stages involved in metacyclogenesis at antigenic level was also evaluated. The results indicate that T. cruzi adapts to various environmental conditions and also that the ability of epimastigotes to undergo metacyclogenesis are influenced by the maintenance schedule. Antigenic profile analysis supports the idea that epimastigotes adapted to culture media do not complete their molecular differentiation into the trypomastigote metacyclic stage. These transition forms conserve some degree of gene expression of the epimastigote stage.
authors.affiliation: Laboratorio de Protozoología, Centro BioMolP, Universidad de Carabobo, Bárbula, Valencia, Estado Carabobo, Venezuela.
authors.forename: Ana R
authors.initials: AR
authors.lastname: De Lima
authors.forename: María C
authors.initials: MC
authors.lastname: Navarro
authors.forename: Rosa Y
authors.initials: RY
authors.lastname: Arteaga
authors.forename: Víctor T
authors.initials: VT
authors.lastname: Contreras
mesh_terms: D000641:Ammonia / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D003470:Culture Media; D005947:Glucose / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D051379:Mice; D009893:Opossums; D012239:Rhodnius; D014349:Trypanosoma cruzi / Q000254:growth & development* / Q000378:metabolism
chemical_list: D003470:Culture Media; D000641:Ammonia; D005947:Glucose