Induction of TNBS colitis in mice.
The animal model described in this unit is one of several that have been used to study the immunopathogenesis of inflammatory bowel disease. The model employs the use of 2,4,6-trinitrobenzenesulfonic acid (TNBS), which induces severe colonic inflammation when administered intrarectally in SJL/J mice. The colitis which results from this procedure presents clinical and histopathological findings that resemble those seen in Crohn's disease. The unit describes the critical parameters needed for successful induction of TNBS-colitis as well methods for monitoring and grading disease levels. A support protocol for isolating lamina propria mononuclear cells from mouse colons is also included.
Scheiffele
Frank
F
National Institute of Allergy and Infectious Disease, NIH, Bethesda, Maryland, USA.
Fuss
Ivan J
IJ
D000818:Animals; D003092:Colitis / Q000139:chemically induced*; D003106:Colon / Q000166:cytology; D004195:Disease Models, Animal; D007963:Leukocytes, Mononuclear / Q000166:cytology; D008198:Lymph Nodes / Q000166:cytology; D051379:Mice; D009092:Mucous Membrane / Q000166:cytology; D014302:Trinitrobenzenesulfonic Acid
D014302:Trinitrobenzenesulfonic Acid