Measuring protein interactions by optical biosensors.
This unit gives an introduction to the basic techniques of optical biosensing for measuring equilibrium and kinetics of reversible protein interactions. Emphasis is given to the description of simple and robust approaches that will provide reliable results. How to avoid the most commonly encountered problems and artifacts is also discussed.
Schuck
P
P
National Institutes of Health, Bethesda, Maryland, USA.
Boyd
L F
LF
Andersen
P S
PS
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D008962:Models, Theoretical; D055095:Optics and Photonics / Q000295:instrumentation*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
D011506:Proteins