[Synergetic effect of B7-1 and CD40L in the immunotherapy for lymphoma].
The aim of study was to investigate the synergetic effect of B7-1 and CD40L co-stimulating pathway in the immunotherapy for lymphoma and to explore the effective manner of tumor vaccine for treating lymphoma. The lymphoma cell line A20 cells were inoculated into BALB/c mice as to establish A20-bearing mice model, the B7-1 and CD40L expression vector were alone or in combination directly injected into lymphoma of mice model, the PBS, vector pcDM8 and pcDNA3.1 were selected as controls so as to observe tumor growth. The pathological section and HE staining of tumor tissue were performed to observe the histological characteristics and the cell infiltration of lymphoma, the CCK-8 detection kit was used to analysis the splenic CTL cytotoxicity. The results showed that the intratumor injection of B7-1 and CD40L resulted in reduction of tumor size. Morphological observation of tumor revealed inflammatory cell infiltration in the tumors, massive necrosis and localization of tumor. CCK-8 kit detection indicated significant enhancement of splenic CTL cytotoxicity, the effect of B7-1 combined with CD40L was stronger than that of B7-1 or CD40L alone. It is concluded that B7-1 and CD40L show immunotherapeutic effect on lymphoma, and the effect becomes stronger when they are combined in treating lymphoma. Meanwhile, the intratumor injection may be considered as a safe and effective way for tumor vaccine.
Zhao
Ling-Zhi
LZ
Department of Hematology, The Third Hospital, Peking University, Beijing 100083, China.
Wang
Jing
J
Li
Min
M
Ke
Xiao-Yan
XY
D000818:Animals; D018122:B7-1 Antigen / Q000235:genetics*; D023201:CD40 Ligand / Q000235:genetics*; D004357:Drug Synergism; D005822:Genetic Vectors; D007167:Immunotherapy / Q000379:methods*; D008223:Lymphoma / Q000473:pathology* / Q000628:therapy*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009368:Neoplasm Transplantation; D014407:Tumor Cells, Cultured
D018122:B7-1 Antigen; D023201:CD40 Ligand