Trace determination of ethyl methanesulfonate in Viracept 250 mg tablets by gas chromatography-mass spectrometry.
Ethyl methanesulfonate (EMS) is a potential human mutagenic and carcinogenic compound which has been found by Roche laboratories in nelfinavir mesylate, the active pharmaceutical ingredient of Viracept. In order to verify the quality of the medicinal product, a gas chromatographic method using mass spectrometry detection was developed for the trace analysis of EMS in Viracept 250 mg tablets from Roche laboratories. Combined with suitable sample preparation including a liquid/liquid extraction this method allows the EMS quantification with a reporting limit of 5 ppm. The extract is injected on a gas chromatographic system with a CP624-CB capillary column. Selected Ion Monitoring mode was used for the EMS quantification. Some validation elements of the method are reported. The validation study was performed over a range from 5 ppm to 100 ppm.
Rebiere
H
H
AFSSAPS - French Health Product Safety Agency - Laboratory and Control Directorate, 34740 Vendargues, France.
Maggio
A-F
AF
Gimeno
P
P
Levaillant
D
D
Civade
C
C
Bonnet
P-A
PA
Tissier
M-H
MH
D002138:Calibration; D005020:Ethyl Methanesulfonate / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D019888:Nelfinavir / Q000032:analysis*; D015203:Reproducibility of Results; D013607:Tablets
D013607:Tablets; D005020:Ethyl Methanesulfonate; D019888:Nelfinavir