title: The GTP binding sites interacted with RNA-dependent RNA polymerase of classical swine fever virus in de novo initiation.
abstract: The NS5B protein of classical swine fever virus (CSFV) is an important enzyme bearing a unique RNA-dependent RNA polymerase (RdRp) activity. The RdRp plays a crucial role in the viral replication cycle and in forming a replicase complex. However, the initiating synthesis mechanism of the CSFV RNA polymerase is unclearly described at present. Our aim is to reveal the RdRp-GTP docking sites and the effective modules of GTP initially bound to the polymerase in starting initiation of replication according to a well predicted CSFV RdRp model. Based on some known crystal structures of RNA polymerase, computational methods were used to establish the model of a CSFV RdRp. An analogous mechanism of CSFV RNA polymerase in de novo initiation was subsequently represented through docking a GTP into the structure model. The unique GTP binding pocket of the polymerase was pointed out: five residues E227, S408, R427, K435, and R439 involved in steady hydrogen bonds and two residues C407 and L232 involved in hydrophobic contact with the GTP. From a genetic evolutionary point of view, three residues C407, S408 and R427 have been suggested to be of particular importance by analysis of residue conservation. It is suggested that these crucial residues should have very significant function in the de novo initiation of the rigorous CSFV polymerase model, which can lead us to design experiments for studying the mechanism of viral replication and develop valid anti-viral drugs.
authors.affiliation: The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China.
authors.forename: Zhuofei
authors.initials: Z
authors.lastname: Xu
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Chao
authors.forename: Youhui
authors.initials: Y
authors.lastname: Si
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Meilin
authors.initials: M
authors.lastname: Jin
authors.forename: Aizhen
authors.initials: A
authors.lastname: Guo
authors.forename: Ping
authors.initials: P
authors.lastname: Qian
authors.forename: Rui
authors.initials: R
authors.lastname: Zhou
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D006692:Classical Swine Fever Virus / Q000201:enzymology* / Q000235:genetics; D006160:Guanosine Triphosphate / Q000378:metabolism*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017510:Protein Folding; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014779:Virus Replication
chemical_list: D012367:RNA, Viral; D006160:Guanosine Triphosphate; D012324:RNA-Dependent RNA Polymerase