Antibody-gold quantum dot-PAMAM dendrimer complex as an immunoglobulin immunoassay.
Gold quantum dots (AuQDs) were synthesized and electrostatically conjugated to goat-derived anti-human IgG for the purpose of detecting human IgG in solution over a broad range of concentrations. The system is able to detect human IgG by linear fluorescence quenching over a micromolar to nanomolar concentration range. We have demonstrated the specificity and a wide dynamic range of the proposed immunoassay. The quenching is a result of competitive surface quenching of the AuQDs. Characterization, details of the immunoassay, and the quenching mechanism, are discussed.
Triulzi
Robert C
RC
University of Miami, Department of Chemistry, 1301 Memorial Drive, Coral Gables, FL 33146, USA.
Micic
Miodrag
M
Orbulescu
Jhony
J
Giordani
Silvia
S
Mueller
Bill
B
Leblanc
Roger M
RM
D000888:Antibodies, Anti-Idiotypic; D050091:Dendrimers; D006046:Gold; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D046529:Microscopy, Electron, Transmission; D011073:Polyamines; D045663:Quantum Dots; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence
D000888:Antibodies, Anti-Idiotypic; D050091:Dendrimers; D007074:Immunoglobulin G; C104700:PAMAM Starburst; D011073:Polyamines; D006046:Gold