title: Immunofluorescence staining.
abstract: The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications (e.g., brightfield, phase-contrast, differential interference contrast, and fluorescence epi-illumination), and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The principles of microscopy and step-by-step alignment and adjustment procedures are described in this unit.
authors.affiliation: National Heart, Lung, and Blood Institute/NIH, Bethesda, Maryland, USA.
authors.forename: Julie G
authors.initials: JG
authors.lastname: Donaldson
mesh_terms: D005455:Fluorescent Antibody Technique; D013194:Staining and Labeling / Q000379:methods*