title: Detection assays for HIV proteins.
abstract: This updated unit describes four assays for detection of HIV proteins including: (1) a quantitative ELISA, (2) a quantitative immunoblotting assay, (3) a qualitative immunofluorescence assay, and (4) a functional assay to measure virus-associated reverse transcriptase activity as an indicator of viral production.
authors.affiliation: University of Toronto, Toronto, Canada.
authors.forename: Mario A
authors.initials: MA
authors.lastname: Ostrowski
authors.affiliation: National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
authors.forename: Tae-Wook
authors.initials: TW
authors.lastname: Chun
authors.affiliation: National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
authors.forename: Bruce
authors.initials: B
authors.lastname: Cheseboro
authors.affiliation: National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
authors.forename: Sharilyn K
authors.initials: SK
authors.lastname: Stanley
authors.affiliation: University of Laval, Quebec, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Tremblay
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D015488:HIV Antigens / Q000032:analysis* / Q000276:immunology; D054303:HIV Reverse Transcriptase / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*
chemical_list: D015488:HIV Antigens; D054303:HIV Reverse Transcriptase