[The experimental study of creating a new rat scarring model by inserting absorbable gelatin sponge into rats' excisional wounds].
OBJECTIVE
To explore the possibility of creating a rat new scar model by inserting gelatin sponge into rat excisional wounds.


METHODS
Two full-thickness wounds were created in each of total 49 SD rats. In the Experimental group (n = 19), a regular incisional wound (1 cm) was created on the left side, and an excisional wound of 1.0 cm x 0.2 cm was created on the right side with a gelatin sponge inserted. In control 1 group (n = 15), an excisional wound with sponge insertion was created on both sides of rats. In control 2 group (n = 15), two excisional wounds were created on both sides, and only one side wounds were inserted with a sponge. Animals were sacrificed at various time points for different examinations.


RESULTS
The wound/scar width increased 4 - 11 times in inserted wounds than in regular incisional wounds (P < 0.01), with an obvious delay of epithelialization. No difference in wound/scar width was found in both sides of wounds of control 1 group at various locations. In contrast to the linear scar of sponge-inserted wounds, contracted and irregular scar was found in non-inserted wounds of control 2 group.


CONCLUSIONS
Gelatin sponge insertion can create a thick linear scar in rat wounds, and thus provides a new model for scar research.
Wu
Xiao-Li
XL
Department of Plastic and Reconstructive Surgery, Shanghai 9th People's Hospital, Shanghai, China.
Liu
Wei
W
Gao
Zhen
Z
Cai
Ze-Hao
ZH
Deng
Dan
D
Zong
Li-Juan
LJ
Yin
De-Min
DM
Cao
Yi-Lin
YL
D000818:Animals; D002921:Cicatrix / Q000473:pathology; D062109:Dermatologic Surgical Procedures; D004195:Disease Models, Animal; D005781:Gelatin Sponge, Absorbable; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology; D013536:Suture Techniques; D014945:Wound Healing