Protein charge determination.
The most popular current method of determining protein valence entails the calculation of net charge from amino acid sequence/composition. However, the inaccuracy of that approach was recognized long before the advent of the protein data banks and computer programs to facilitate its adoption. Capillary zone electrophoresis affords the simplest and most economical procedure for obtaining a reliable estimate of the net charge of a protein in the buffer system of interest. This unit explains the major pitfalls in the calculation of net charge from protein sequence data.
Winzor
Donald J
DJ
University of Queensland, Brisbane, Queensland.
D000596:Amino Acids / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods; D011506:Proteins / Q000737:chemistry*
D000596:Amino Acids; D011506:Proteins