Changes on histone H3 modifications on the GLUT5 gene and its expression in Caco-2 cells co-treated with a p44/42 MAPK inhibitor and glucocorticoid hormone.
Changes on histone H3 modifications from methylation at lysine 9 to acetylation at lysine 9/14 is thought to be important for transcriptional activation of genes associated with differentiation. In this study, we found that the treatment with a p44/42 MAPK inhibitor, PD98059, induced di-methylation at lysine 9 on the upstream/transcriptional regions of the GLUT5 gene. Co-treatment of PD98059 with a glucocorticoid hormone agonist, dexamethasone (Dex), not only repressed the induction of di-methylation by PD98059, but also enhanced the acetylation of histone H3 at lysine 9/14 on the GLUT5 gene, as well as its gene expression. These results suggest that the histone H3 di-methylation at lysine 9, as well as acetylation at lysine 9/14, may be indispensable for coordinated induction of the GLUT5 gene by p44/42 MAP kinase inhibition and the glucocorticoid hormone.
Mochizuki
Kazuki
K
Laboratory of Nutritional Physiology, The University of Shizuoka, Graduate School of Nutritional and Environmental Sciences and Global COE, 52-1 Yada, Shizuoka-shi, Shizuoka 422-8526, Japan.
Takabe
Satsuki
S
Goda
Toshinao
T
D000107:Acetylation / Q000187:drug effects; D018938:Caco-2 Cells; D003907:Dexamethasone / Q000378:metabolism / Q000494:pharmacology; D005419:Flavonoids / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D005786:Gene Expression Regulation; D005938:Glucocorticoids / Q000378:metabolism / Q000494:pharmacology; D051296:Glucose Transporter Type 5 / Q000235:genetics*; D006657:Histones / Q000187:drug effects / Q000378:metabolism*; D006801:Humans; D008239:Lysine / Q000378:metabolism; D008745:Methylation / Q000187:drug effects; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000494:pharmacology
D005419:Flavonoids; D005938:Glucocorticoids; D051296:Glucose Transporter Type 5; D006657:Histones; D047428:Protein Kinase Inhibitors; C495726:SLC2A5 protein, human; D003907:Dexamethasone; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3; D008239:Lysine; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one