Construction of a reporter vector system for in vivo analysis of promoter activity in Propionibacterium freudenreichii.
A beta-galactosidase reporter system for the analysis of promoter elements in Propionibacterium freudenreichii was designed. The pTD210 in vivo reporter vector was constructed using a promoterless lacZ gene from Bifidobacterium longum cloned into the pAMT1 plasmid. The utility of the pTD210 reporter vector was demonstrated by an investigation of six predicted promoters in P. freudenreichii. The system produced accurate and reproducible measurements that facilitated both promoter identification and the quantification of promoter activities.
Faye
Therese
T
Laboratory of Microbial Gene Technology and Food Microbiology, Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences (UMB), PO Box 5003, N-1432 As, Norway.
Asebø
Anita
A
Salehian
Zhian
Z
Langsrud
Thor
T
Nes
Ingolf F
IF
Brede
Dag Anders
DA
D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics; D001644:Bifidobacterium / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005822:Genetic Vectors; D008969:Molecular Sequence Data; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011424:Propionibacterium / Q000235:genetics*; D017422:Sequence Analysis, DNA; D001616:beta-Galactosidase / Q000096:biosynthesis / Q000235:genetics
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001616:beta-Galactosidase