Evaluation of 8-hydroxydeoxyguanosine, thiobarbituric acid-reactive substances and total antioxidant status as possible disease markers in oesophageal malignancies.
OBJECTIVE
Evaluation of oxidative stress and diagnostic utility of its markers in oesophageal squamous cell carcinoma (OSCC).


METHODS
Serum 8-hydroxydeoxyguanosine, thiobarbituric acid-reactive substances (TBARS) and total antioxidant status (TAS) were measured in OSCC (n=75), non-malignant oesophageal diseases (n=30), and healthy subjects (n=79). Three months following oesophagectomy the measurements were repeated.


RESULTS
Exclusively in OSCC, 8-hydroxydeoxyguanosine and TBARS were elevated. TAS was reduced in non-malignancies compared to controls, and in OSCC compared to non-malignancies and controls. Only 8-hydroxydeoxyguanosine was associated with disease progression, lymph node involvement in particular. All indices were good indicators of cancer presence (ROC analysis) and normalized following oesophagectomy. A positive linear relationship between 8-hydroxydeoxyguanosine and TBARS, and negative non-linear between TAS and both 8-hydroxydeoxyguanosine and TBARS was demonstrated.


CONCLUSIONS
OSCC is associated with oxidative stress, attenuated following oesophagectomy. Consumption of serum antioxidants prevents accumulation of oxidatively modified molecules in non-malignancies. High accuracy of oxidative stress markers in indicating cancer presence warrants further investigation on their possible application as discriminatory markers and in monitoring treatment efficacy.
Diakowska
Dorota
D
Department of Gastrointestinal and General Surgery, Wroclaw Medical University, Wroclaw, Poland. ddiakow@chir.am.wroc.pl
Krzystek-Korpacka
Malgorzata
M
Lewandowski
Andrzej
A
Grabowski
Krzysztof
K
Diakowski
Witold
W
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood* / Q000000981:diagnostic imaging / Q000473:pathology; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000097:blood; D003937:Diagnosis, Differential; D004935:Esophageal Diseases / Q000097:blood*; D004938:Esophageal Neoplasms / Q000097:blood* / Q000000981:diagnostic imaging / Q000473:pathology; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology; D009367:Neoplasm Staging; D018384:Oxidative Stress / Q000502:physiology; D011859:Radiography; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism*
D000975:Antioxidants; D015415:Biomarkers; D014408:Biomarkers, Tumor; D017392:Thiobarbituric Acid Reactive Substances; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine