title: Probing nucleic acid structure with shape-selective rhodium and ruthenium complexes.
abstract: In this unit, transition metal complexes are used as photochemical probes for the structure of RNA and DNA. The transition metal ion provides a rigid substitutionally inert framework and an octahedral geometry for ligand coordination. The complexes can be constructed to define shapes, symmetries, and functionalities that complement those of the nucleic acid target. Complex formation is easily detected by light-induced nucleic acid cleavage. The modular construction of the complexes makes it possible to generate probes to examine a wide variety of structural characteristics of nucleic acids.
authors.affiliation: California Institute of Technology, Pasadena, California, USA.
authors.forename: B A
authors.initials: BA
authors.lastname: Jackson
authors.forename: J K
authors.initials: JK
authors.lastname: Barton
mesh_terms: D020137:Base Pair Mismatch; D001671:Biochemistry / Q000379:methods*; D004247:DNA / Q000737:chemistry; D018983:DNA Footprinting; D006147:Guanine / Q000737:chemistry; D009690:Nucleic Acid Conformation / Q000187:drug effects; D009696:Nucleic Acids / Q000737:chemistry*; D010618:Phenanthrolines / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D010782:Photolysis; D012313:RNA / Q000737:chemistry; D012238:Rhodium / Q000737:chemistry* / Q000494:pharmacology; D012428:Ruthenium / Q000737:chemistry* / Q000494:pharmacology; D017975:Ruthenium Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D026082:Singlet Oxygen / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D009696:Nucleic Acids; D010618:Phenanthrolines; D017975:Ruthenium Compounds; D026082:Singlet Oxygen; D006147:Guanine; D012313:RNA; D012428:Ruthenium; D004247:DNA; D012238:Rhodium