title: The C, V and W proteins of Nipah virus inhibit minigenome replication.
abstract: Nipah virus (NiV) is a recently emergent, highly pathogenic, zoonotic paramyxovirus of the genus Henipavirus. Like the phosphoprotein (P) gene of other paramyxoviruses, the P gene of NiV is predicted to encode three additional proteins, C, V and W. When the C, V and W proteins of NiV were tested for their ability to inhibit expression of the chloramphenicol acetyltransferase (CAT) reporter gene in plasmid-based, minigenome replication assays, each protein inhibited CAT expression in a dose-dependent manner. The C, V and W proteins of NiV also inhibited expression of CAT from a measles virus (MV) minigenome, but not from a human parainfluenzavirus 3 (hPIV3) minigenome. Interestingly, the C and V proteins of MV, which have previously been shown to inhibit MV minigenome replication, also inhibited NiV minigenome replication; however, they were not able to inhibit hPIV3 minigenome replication. In contrast, the C protein of hPIV3 inhibited minigenome replication of hPIV3, NiV and MV. Although there is very limited amino acid sequence similarity between the C, V and W proteins within the paramyxoviruses, the heterotypic inhibition of replication suggests that these proteins may share functional properties.
authors.affiliation: Measles, Mumps, Rubella, and Herpesvirus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Katrina
authors.initials: K
authors.lastname: Sleeman
authors.affiliation: Measles, Mumps, Rubella, and Herpesvirus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Bettina
authors.initials: B
authors.lastname: Bankamp
authors.affiliation: Measles, Mumps, Rubella, and Herpesvirus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Kimberly B
authors.initials: KB
authors.lastname: Hummel
authors.affiliation: Emory University, Atlanta, GA, USA.|The Research Institute, Nationwide Children's Hospital, Columbus, OH, USA.|Measles, Mumps, Rubella, and Herpesvirus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Lo
authors.affiliation: Measles, Mumps, Rubella, and Herpesvirus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: William J
authors.initials: WJ
authors.lastname: Bellini
authors.affiliation: Measles, Mumps, Rubella, and Herpesvirus Laboratory Branch, Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Paul A
authors.initials: PA
authors.lastname: Rota
mesh_terms: D000818:Animals; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000096:biosynthesis / Q000235:genetics; D006224:Cricetinae; D017930:Genes, Reporter; D016679:Genome, Viral; D008459:Measles virus / Q000235:genetics / Q000502:physiology; D045405:Nipah Virus / Q000235:genetics / Q000502:physiology*; D010224:Parainfluenza Virus 3, Human / Q000235:genetics / Q000502:physiology; D014764:Viral Proteins / Q000378:metabolism*; D014779:Virus Replication / Q000235:genetics / Q000502:physiology*
chemical_list: D014764:Viral Proteins; D015500:Chloramphenicol O-Acetyltransferase