Vaccinia-based reporter gene cell-fusion assays to quantitate functional interactions of HIV envelope glycoprotein with receptors.
This unit describes quantitation of functional interactions between HIV envelope glucoprotein and target cell receptors, using assay of cell fusion-dependent reporter gene activation. The method is particularly useful since it isolates the fusion reaction from the rest of the HIV replication cycle, and obviates the need for infectious HIV particles. Reporter Gene Cell Fusion Assays to Quantitate Functional Interactions of HIV Envelope Glycoprotein with Receptors.
Dey
Barna
B
National Institutes of Health, Bethesda, Maryland, USA.
Berger
Edward A
EA
D000818:Animals; D002459:Cell Fusion / Q000379:methods*; D017930:Genes, Reporter; D006678:HIV / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D015728:Receptors, HIV / Q000378:metabolism*; D014616:Vaccinia virus / Q000235:genetics*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000378:metabolism*
D015728:Receptors, HIV; D054299:env Gene Products, Human Immunodeficiency Virus