Measurement of interleukin 3 and other hematopoietic cytokines, such as GM-CSF, G-CSF, M-CSF, erythropoietin, steel factor, and Flt-3 ligand.
Detection and quantitation of IL-3 and other hematopoietic cytokines in serum samples and culture supernatants has played an important role in understanding their function and pleiotropic properties during the process of hematopoiesis. Several methods for detecting and quantitating cytokines--such as the colony-stimulating factors granulocyte/macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), macrophage colony-stimulating factor (M-CSF, also called CSF-1), interleukin 3 (IL-3), and erythropoietin (Epo), as well as two potent costimulating cytokines steel factor (SLF) and Flt3/Flk-2 ligand (Flt3-L)--are described in this unit.
Cooper
S H
SH
Indiana University School of Medicine, Indianapolis, Indiana, USA.
Broxmeyer
H E
HE
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001854:Bone Marrow Cells / Q000502:physiology; D017077:Culture Media, Conditioned; D016207:Cytokines / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004921:Erythropoietin / Q000032:analysis; D016179:Granulocyte Colony-Stimulating Factor / Q000032:analysis; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000032:analysis; D006801:Humans; D007377:Interleukin-3 / Q000032:analysis*; D007963:Leukocytes, Mononuclear / Q000502:physiology; D016173:Macrophage Colony-Stimulating Factor / Q000032:analysis; D008565:Membrane Proteins / Q000032:analysis; D051379:Mice; D019089:Stem Cell Factor / Q000032:analysis
D017077:Culture Media, Conditioned; D016207:Cytokines; D007377:Interleukin-3; D008565:Membrane Proteins; D019089:Stem Cell Factor; C084881:flt3 ligand protein; D004921:Erythropoietin; D016179:Granulocyte Colony-Stimulating Factor; D016173:Macrophage Colony-Stimulating Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor