Effect of a toxicant on phagocytosis pathways in the freshwater snail Lymnaea stagnalis.
The disturbance of plasma membrane carbohydrates and of lipopolysaccharide (LPS) ligands in relation to cytoskeletal transformations of haemocytes has been investigated after chronic exposure of pond snails (Lymnaea stagnalis) to the peroxidizing toxicant fomesafen. Neither of the two lectins used (concanavalin A and wheat germ agglutinin) showed any binding modification after incubation of the snails in the presence of the toxicant. However, after exposure of the snails to fomesafen, a clear and persistent reduction in LPS labelling of haemocytes occurred. The actin cytoskeleton of the same cells also appeared to be sensitive to the toxicant. The reduction in LPS-binding sites was related to actin staining, leading to the hypothesis that LPS ligands and actin could be similarly modulated by the toxicant. Damaged cells showed non-adherent membrane portions with reduced filopodial extrusions, exhibiting a smooth surface free of microvilli. These changes could lower the spreading and adhesion of the cells and could therefore account for the loss in their phagocytic capabilities.
Russo
Jacqueline
J
UMR 6553 Ecobio, Université Rennes 1, CNRS, Campus de Beaulieu, Avenue du Général Leclerc, 35042, Rennes, France. jacqueline.russo@univ-rennes1.fr
Madec
Luc
L
Brehélin
Michel
M
D000199:Actins / Q000378:metabolism; D000818:Animals; D001549:Benzamides / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D016650:Fluorescein-5-isothiocyanate / Q000378:metabolism; D005456:Fluorescent Dyes / Q000378:metabolism; D006434:Hemocytes / Q000187:drug effects / Q000276:immunology / Q000378:metabolism / Q000648:ultrastructure; D006540:Herbicides / Q000633:toxicity; D037102:Lectins / Q000276:immunology / Q000378:metabolism / Q000494:pharmacology; D008195:Lymnaea / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D010587:Phagocytosis / Q000187:drug effects* / Q000276:immunology; D010590:Phalloidine / Q000378:metabolism; D012235:Rhodamines / Q000378:metabolism; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000199:Actins; D001549:Benzamides; D005456:Fluorescent Dyes; D006540:Herbicides; D037102:Lectins; D012235:Rhodamines; D014874:Water Pollutants, Chemical; D010590:Phalloidine; C009434:tetramethylrhodamine isothiocyanate; D016650:Fluorescein-5-isothiocyanate; C088563:fomesafen