[Isolation and identification in Senegal of the most immunogenic protein soluble antigen of a Clostridium chauvoei strain].
Clostridium chauvoei is the pathogenic agent for blackleg, a toxinfection disease in bovine and small ruminants, always lethal and involving considerable economic losses. Some bacteriological, biochemical, immunological studies permitted to isolate identify the major soluble antigenic protein of this bacteria. It's a protein fragment of 70 kDa weight, the 19 fraction, excreted by the bacteria in a suitable culture medium. The 19 fraction of extracellular medium leads to antibodies production on guinea pigs revealed by the ELISA/antibody test.
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Institut sénégalais de recherches agricoles, Laboratoire national de l'levage et de recherches v4tbrinaires. BP 2057, Dakar Hann, Sénégal. mbenguem@hotmail.comn
D000818:Animals; D000907:Antibodies, Bacterial / Q000096:biosynthesis / Q000276:immunology; D000942:Antigens, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D001426:Bacterial Proteins / Q000032:analysis / Q000276:immunology* / Q000302:isolation & purification; D046975:Clostridium chauvoei / Q000276:immunology*; D003470:Culture Media; D017077:Culture Media, Conditioned; D004797:Enzyme-Linked Immunosorbent Assay; D005407:Flagella / Q000276:immunology; D006168:Guinea Pigs; D007114:Immunization; D051379:Mice; D008970:Molecular Weight; D010446:Peptide Fragments / Q000032:analysis / Q000276:immunology / Q000302:isolation & purification; D012675:Senegal
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D003470:Culture Media; D017077:Culture Media, Conditioned; D010446:Peptide Fragments