Cloud point extraction and flame atomic absorption spectrometry combination for copper(II) ion in environmental and biological samples.
A cloud point extraction procedure was presented for the preconcentration of copper(II) ion in various samples. After complexation by 4-(phenyl diazenyl) benzene-1,3-diamine (PDBDM) (chrysoidine), copper(II) ions were quantitatively recovered in Triton X-114 after centrifugation. 0.5ml of methanol acidified with 1.0molL(-1) HNO3 was added to the surfactant-rich phase prior to its analysis by flame atomic absorption spectrometry (FAAS). The influence of analytical parameters including ligand, Triton X-114 and HNO3 concentrations, bath temperature, heating time, centrifuge rate and time were optimized. The effect of the matrix ions on the recovery of copper(II) ions was investigated. The detection limit (3S.D.b/m, n=10) of 0.6ngmL(-1) along with preconcentration factor of 30 and enrichment factor of 41.1 with R.S.D. of 1.0% for Cu was achieved. The proposed procedure was applied to the analysis of various environmental and biological samples.
Shokrollahi
Ardeshir
A
Chemistry Department, Yasouj University, Yasouj 75914-353, Iran. ashokrollahi@mail.yu.ac.ir
Ghaedi
Mehrorang
M
Hossaini
Omid
O
Khanjari
Narges
N
Soylak
Mustafa
M
D000818:Animals; D001774:Blood Chemical Analysis; D002138:Calibration; D002498:Centrifugation; D004396:Coloring Agents; D003300:Copper / Q000032:analysis* / Q000302:isolation & purification; D004785:Environmental Pollutants / Q000032:analysis*; D005504:Food Analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007202:Indicators and Reagents; D008823:Micelles; D017830:Octoxynol; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D013054:Spectrophotometry, Atomic; D013696:Temperature; D014881:Water Supply / Q000032:analysis; D010128:p-Aminoazobenzene / Q000031:analogs & derivatives / Q000737:chemistry
D004396:Coloring Agents; D004785:Environmental Pollutants; D007202:Indicators and Reagents; D008823:Micelles; C005562:chrysoidine; D011092:Polyethylene Glycols; D012965:Sodium Chloride; D010128:p-Aminoazobenzene; D003300:Copper; D017830:Octoxynol; C010615:Nonidet P-40