Use of commercial cards for freeing DNA from mycobacterial strains.
A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.
Tortoli
Enrico
E
Regional Reference Center for Mycobacteria, Careggi Hospital, Florence, Italy. enrico.tortoli@unifi.it
Baruzzo
Serena
S
Lauria
Stefania
S
Mariottini
Alessandro
A
D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics / Q000302:isolation & purification*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D009161:Mycobacterium / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D011933:Reagent Kits, Diagnostic; D017422:Sequence Analysis, DNA; D013048:Specimen Handling / Q000295:instrumentation / Q000379:methods*
D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic