Increased abundance of cytoplasmic and nuclear caveolin 1 in human diploid fibroblasts in H(2)O(2)-induced premature senescence and interplay with p38alpha(MAPK).
Treatment of IMR-90 human diploid fibroblasts with a sublethal concentration of H(2)O(2) induces premature senescence. We investigated the protein abundance, subcellular localization and involvement of caveolin 1 in premature senescence. Caveolin 1 is a scaffolding protein able to concentrate and organize signaling molecules within the caveolae membrane domains. We report the first evidence of increased nuclear and cytoplasmic localization of caveolin 1 during establishment of H(2)O(2)-induced premature senescence. Moreover, we demonstrate that phosphorylation of caveolin 1 during treatment with H(2)O(2) is dependent on p38alpha mitogen-activated protein kinase.
Chrétien
Aline
A
Unit of Research on Cellular Biology, University of Namur (FUNDP), Rue de Bruxelles 61, B-5000 Namur, Belgium.
Piront
Neil
N
Delaive
Edouard
E
Demazy
Catherine
C
Ninane
Noëlle
N
Toussaint
Olivier
O
D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D016922:Cellular Senescence; D003593:Cytoplasm / Q000378:metabolism*; D004171:Diploidy; D015536:Down-Regulation; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D048308:Mitogen-Activated Protein Kinase 14 / Q000235:genetics / Q000378:metabolism*; D018384:Oxidative Stress; D034741:RNA, Small Interfering / Q000235:genetics
D051242:Caveolin 1; D034741:RNA, Small Interfering; D006861:Hydrogen Peroxide; D048308:Mitogen-Activated Protein Kinase 14