[Optimization of cultivation conditions of the alpha-amylase producer Bacillus subtilis 147].
The influence of some technological parameters of cultivation of the producer Bacillus subtilis 147 on the process of extracellular enzymes alpha-amylase synthesis was investigated. The optimum sources of carbon (0.1% insoluble starch) and nitrogen (0.2% sodium nitrate) for maximum production of alpha-amylase are established. It was shown, that the temperature 42 degrees C, the carbon nitrogen ratio 1:2, pH 7.0, volume of a nutritious medium 100 ml, rotation rate 220 rev/min during 4-6 days are optimum parameters of the producer cultivation. As a result the enzyme activity was increased 14 times.
Avdiiuk
K V
KV
Varbanets'
L D
LD
D001412:Bacillus subtilis / Q000201:enzymology / Q000254:growth & development*; D018533:Biomass; D002244:Carbon / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D009584:Nitrogen / Q000737:chemistry; D013696:Temperature; D000516:alpha-Amylases / Q000096:biosynthesis / Q000302:isolation & purification / Q000378:metabolism
D003470:Culture Media; D002244:Carbon; D000516:alpha-Amylases; D009584:Nitrogen