Purification of glutamate dehydrogenase from liver and brain.
Two alternative procedures are described for the purification of the major form of glutamate dehydrogenase (L-glutamate-NAD(P)+ oxidoreductase (deaminating), EC 1.4.1.3: GDH) from ox liver and brain. The first involves affinity chromatography on a column of the allosteric inhibitor GTP bound to Sepharose, whereas the other uses a bifunctional ligand (bis-NAD+) composed of two NAD+ molecules linked together by a spacer arm to precipitate the enzyme in the presence of the substrate analogue glutarate. In both procedures the affinity steps are preceded by ammonium sulfate precipitation and ion exchange chromatography on DEAE cellulose. Procedures for the synthesis of GTP-Sepharose and bis-NAD+ are described and the ancillary procedures, including the assay of GDH activity and the determination of protein concentration, are also presented.
Motherway
Martha
M
Department of Biochemistry Trinity College, Dublin, Ireland.
Tipton
Keith F
KF
Department of Biochemistry Trinity College, Dublin, Ireland.
McCarthy
Alun D
AD
GlaxoSmithKline, Middlesex, United Kingdom.
Couée
Ivan
I
Centre National de la Recherche Scientifique, Rennes, France.
Irwin
Jane
J
University College Dublin, Dublin, Ireland.
D000818:Animals; D001921:Brain / Q000201:enzymology*; D002417:Cattle; D002846:Chromatography, Affinity / Q000379:methods; D005969:Glutamate Dehydrogenase / Q000302:isolation & purification*; D006160:Guanosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D008099:Liver / Q000201:enzymology*; D015394:Molecular Structure; D009243:NAD / Q000737:chemistry / Q000378:metabolism; D012685:Sepharose / Q000737:chemistry / Q000378:metabolism
D009243:NAD; D006160:Guanosine Triphosphate; D012685:Sepharose; D005969:Glutamate Dehydrogenase