title: Analysis on the emerging role of Rab3 GTPase-activating protein in Warburg Micro and Martsolf syndrome.
abstract: Evidence is accumulating that Rab3A plays a key role in neurotransmitter release and synaptic plasticity. Recently mutations in the catalytic subunit p130 and the noncatalytic subunit p150 of Rab3 GTPase-activating protein were found to cause Warburg Micro syndrome and Martsolf syndrome, respectively, both of which exhibit mental retardation. We have found that loss of p130 in mice results in inhibition of Ca2+-dependent glutamate release from cerebrocortical synaptosomes and alters short-term plasticity in the hippocampal CA1 region, probably through the accumulation of the GTP-bound form of Rab3A. Here, we describe the procedures for the measurement of the GTP-bound pool of Rab3A with pull-down assay using mouse brains and the biochemical method for the measurement of glutamate release from mouse synaptosomes.
authors.affiliation: Department of Biochemistry, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
authors.forename: Ayuko
authors.initials: A
authors.lastname: Sakane
authors.forename: Jun
authors.initials: J
authors.lastname: Miyoshi
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Takai
authors.forename: Takuya
authors.initials: T
authors.lastname: Sasaki
mesh_terms: D000015:Abnormalities, Multiple / Q000235:genetics; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006224:Cricetinae; D018698:Glutamic Acid / Q000378:metabolism; D006801:Humans; D051379:Mice; D013574:Synaptosomes / Q000378:metabolism; D013577:Syndrome; D020692:rab3 GTP-Binding Proteins / Q000172:deficiency; D020826:rab3A GTP-Binding Protein / Q000032:analysis / Q000502:physiology*
chemical_list: D018698:Glutamic Acid; C512588:Rab3 GTPase-activating protein p130, mouse; D020692:rab3 GTP-Binding Proteins; D020826:rab3A GTP-Binding Protein; D002118:Calcium