Rapid and efficient DNA strand cross-linking by click chemistry.
Click chemistry has been used to covalently cross-link complementary DNA strands between bases to form very stable duplexes. Several alkyne- and azide-modified uracil monomers were used to evaluate the effect of the linkers on the efficiency of the click reaction. All cross-linked duplexes had much higher thermal stabilities than non-cross-linked ones, with increases in melting temperature of up to 30 degrees C. In some cases, the conversion was near-quantitative, and the reaction was complete in 5 min.
Kocalka
Petr
P
School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, UK.
El-Sagheer
Afaf H
AH
Brown
Tom
T
D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002942:Circular Dichroism; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D003857:Deoxyuridine / Q000737:chemistry; D019075:Electrophoresis, Capillary; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry; D013997:Time Factors
D000480:Alkynes; D001386:Azides; D003432:Cross-Linking Reagents; D009841:Oligonucleotides; D004247:DNA; D003857:Deoxyuridine