Identification and characterization of nonapeptide targeting a human B cell lymphoma, Raji.
Here, we identified a novel peptide specifically targeting a human B cell lymphoma, Raji, through a conventional phage display method. The amino acid sequence, 'CTLPHLKMC' was obtained with the highest frequency from a nonapeptide-expressing phage library. The phage clone encoding CTLPHLKMC peptide sequence avidly bound to Raji cells compared with control phage clones. Furthermore, flow-cytometric analysis on the biotinylated synthetic CTLPHLKMC peptide demonstrated the high binding affinity to Raji cells in a dose-dependent manner whereas it has binding activity to neither human peripheral blood mononuclear cells including normal B cell derived from healthy donors nor other leukemia cells including THP-1, HL-60, Jurkat and IM-9. MALDI-TOF mass spectrometry following immunoprecipitation assay showed that a potential host receptor for the peptide is a variable region of human immunoglobulin heavy chain which would be a specific phenotypic marker of Raji. In conclusion, these results suggest that the peptide, 'CTLPHLKMC', is a specific ligand to a Raji cell.
Choi
Jin Huk
JH
Laboratory of Protein Engineering and Comparative Immunology, School of Agricultural Biotechnology, Seoul National University, Seoul 151-921, Republic of Korea.
Lee
Woon Kyu
WK
Han
Seung Hyun
SH
Ha
Seckho
S
Ahn
Sung Min
SM
Kang
Jae Seong
JS
Choi
Yun Jaie
YJ
Yun
Cheol-Heui
CH
D014408:Biomarkers, Tumor / Q000378:metabolism*; D002051:Burkitt Lymphoma / Q000378:metabolism*; D045744:Cell Line, Tumor; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000378:metabolism*; D008024:Ligands; D009842:Oligopeptides / Q000378:metabolism*; D019151:Peptide Library; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D014408:Biomarkers, Tumor; D007143:Immunoglobulin Heavy Chains; D008024:Ligands; D009842:Oligopeptides; D019151:Peptide Library