Attachment of nucleosides to solid-phase supports.
Specific step-by-step instructions are given for coupling nucleosides to LCAA-CPG supports (supports consisting of a long-chain alkylamine linked to controlled-pore glass). Protocols are given for a succinic acid linker and a hydroquinone-O,O'-diacetic acid linker. The former is the most widely used linker arm, and the starting materials are widely available. The latter offers greater compatibility with base-sensitive sequence modifications and great synthetic throughput because it can be cleaved under milder and faster conditions. Additional guidelines are given for selecting a linker arm and coupling protocol. Almost any application requiring synthetic oligonucletodies can be satisfied using one of these linker arms.
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University of Calgary, Calgary, Alberta, Canada.
D001671:Biochemistry / Q000295:instrumentation / Q000379:methods*; D002264:Carboxylic Acids / Q000737:chemistry; D004952:Esters / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D006873:Hydroquinones / Q000737:chemistry; D006900:Hydroxylation; D009596:Nitrophenols / Q000737:chemistry; D009705:Nucleosides / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D016062:Porosity; D012995:Solubility; D019802:Succinic Acid / Q000737:chemistry; D014320:Triphenylmethyl Compounds / Q000737:chemistry
D002264:Carboxylic Acids; D004952:Esters; D006873:Hydroquinones; D009596:Nitrophenols; D009705:Nucleosides; D011108:Polymers; D014320:Triphenylmethyl Compounds; D019802:Succinic Acid; C031927:hydroquinone