Single-sperm typing.
This unit presents protocols for sperm isolation using two different methods, amplification of simple sequence-length polymorphisms (SSLP) and/or single nucleotide polymorphisms (SNP) from single cells or whole genome-amplified single cells using primer extension preamplification (PEP), and discusses the statistical analysis of sperm-typing recombination data. Newer methods for studying recombination over very short distances (a few kilobases) using total sperm DNA and allele-specific PCR are also discussed.
Lien
Sigbjorn
S
Agricultural University of Norway, Aas, Norway.
Szyda
Joanna
J
Leeflang
Esther P
EP
Hubert
Rene
R
Zhang
Lin
L
Schmitt
Karin
K
Arnheim
Norman
N
D002469:Cell Separation; D005434:Flow Cytometry; D005819:Genetic Markers; D005821:Genetic Techniques; D005826:Genetics, Medical; D006239:Haplotypes; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction; D012685:Sepharose; D017422:Sequence Analysis, DNA / Q000379:methods; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism*
D005819:Genetic Markers; D012685:Sepharose