Fluorescence resonance energy transfer: techniques for measuring molecular conformation and molecular proximity.
This overview unit focuses on the basics of fluorescence and of the FRET phenomenon, and on methods for detecting FRET and data interpretation. FRET is very versatile and there are more application of these basics than can be covered in a single overview. However, some examples are given of applications of various FRET techniques.
Edidin
Michael
M
Johns Hopkins University, Baltimore, Maryland, USA.
D000818:Animals; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods* / Q000639:trends*; D006801:Humans; D008968:Molecular Conformation