Effect of BCG stimulus on proinflammatory cytokine production in laryngeal cancer.
BACKGROUND
Evaluate the production of TNF and IL-6 in the supernatant of peripheral blood mononuclear cell (PBMC) cultures of patients with supraglottic laryngeal cancer before and after surgical treatment.


METHODS
Adherent cell cultures were stimulated with LPS and BCG. Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cytokine concentration was determined by ELISA in supernatants of mononuclear cell cultures.


RESULTS
In non-stimulated cultures, lower TNF cytokine levels were detected during the late postoperative (LP) period compared to control (P = 0.02). LP TNF and IL-6 levels were high in cultures stimulated with LPS compared with the preoperative period (PREOP) (P = 0.007; P = 0.008, respectively). Stimulation with BCG led to increased levels of TNF and IL-6 during the LP period compared to control (P = 0.001; P = 0.04, respectively).


CONCLUSIONS
BCG is able to modulate the immune response of patients with advanced supraglottic laryngeal cancer in vitro, increasing the secretion of TNF and IL-6 by macrophages during the postoperative period.
Conti-Freitas
Luiz Carlos
LC
Department of Head and Neck Surgery, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil. lcconti@uol.com.br
Foss-Freitas
Maria Cristina
MC
Mamede
Rui Celso Martins
RC
Foss
Norma Tiraboschi
NT
D000328:Adult; D000368:Aged; D001500:BCG Vaccine / Q000276:immunology / Q000627:therapeutic use*; D002277:Carcinoma / Q000188:drug therapy*; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D005260:Female; D006801:Humans; D007249:Inflammation; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012907:Smoking; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000097:blood / Q000276:immunology
D001500:BCG Vaccine; D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha