title: Systemic control of plasmacytoid dendritic cells by CD8+ T cells and commensal microbiota.
abstract: The composition of the intestinal microbial community is a distinctive individual trait that may divergently influence host biology. Because dendritic cells (DC) regulate the quality of the host response to microbiota, we evaluated DC in mice bearing distinct enteric microbial communities divergent for colitis susceptibility. Surprisingly, a selective, systemic reduction of plasmacytoid dendritic cells (pDC) was observed in isogenic mice with different microbiota: restricted flora (RF) vs specific pathogen free (SPF). This reduction was not observed in germfree mice, suggesting that the pDC deficiency was not simply due to a lack of intestinal microbial products. The microbial action was linked to cytotoxic CD8(+) T cells, since pDC in RF mice were preserved in the CD8(-/-) and perforin(-/-) genotypes, partially restored by anti-CD8beta Ab, and augmented in SPF mice bearing the TAP(-/-) genotype. Direct evidence for pDC cytolysis was obtained by rapid and selective pDC depletion in SPF mice transferred with RF CD8(+) T cells. These data indicate that commensal microbiota, via CTL activation, functionally shape systemic immune regulation that may modify risk of inflammatory disease.
authors.affiliation: Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California-Los Angeles, CA 90095, USA.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Fujiwara
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.forename: Laura L
authors.initials: LL
authors.lastname: Presley
authors.forename: Sarah
authors.initials: S
authors.lastname: Brewer
authors.forename: Michael
authors.initials: M
authors.lastname: McPherson
authors.forename: Michael A
authors.initials: MA
authors.lastname: Lewinski
authors.forename: James
authors.initials: J
authors.lastname: Borneman
authors.forename: Jonathan
authors.initials: J
authors.lastname: Braun
mesh_terms: D000818:Animals; D001419:Bacteria / Q000276:immunology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D003092:Colitis / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D007422:Intestines / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D018345:Mice, Knockout; D054353:Perforin / Q000235:genetics / Q000276:immunology; D010950:Plasma Cells / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms / Q000276:immunology
chemical_list: D054353:Perforin