Isolation of genomic DNA from mammalian cells.
This unit describes simple, cost-effective preparation of DNA from whole blood or cultured cells that yields high-molecular-weight DNA suitable for both Southern blotting and the polymerase chain reaction. Preparation time may be shortened by substituting a high-salt precipitation procedure for the dialysis step; however, this results in a smaller average fragment size. The isolation of DNA from buccal swabs, collected from the inside of the cheek, is also described. The DNA is suitable for PCR analysis. Preparation of buffered phenol for DNA extraction is described in a support protocol. This unit describes simple, cost-effective preparation of DNA from whole blood or cultured cells that yields high-molecular-we.
Gilbert
J R
JR
Duke University Medical Center, Durham, North Carolina, USA.
Vance
J M
JM
D002478:Cells, Cultured; D004247:DNA / Q000097:blood / Q000235:genetics / Q000302:isolation & purification*; D005821:Genetic Techniques; D005826:Genetics, Medical; D006801:Humans; D009061:Mouth Mucosa / Q000737:chemistry
D004247:DNA