Effects of ethanol consumption on periodontal inflammation in rats.
Studies suggest a correlation between ethanol consumption and periodontal disease. We hypothesized that elevated levels of blood reactive oxygen species following ethanol consumption may increase inflammation in periodontal tissue. Rats were divided into 4 groups (6-7 rats/group). Two groups were fed an ethanol-containing liquid diet, and 2 groups were fed a pair-fed control diet. In one of each dietary group, periodontitis was ligature-induced, while the other group was left unligated. Chronic ethanol feeding alone decreased the ratio of reduced/oxidized glutathione and increased 8-hydroxydeoxy-guanosine and tumor necrosis factor (TNF)-alpha levels in the gingiva. Blood hydroperoxides were also increased. In ligature-induced periodontitis lesions, ethanol feeding enhanced polymorpho-nuclear leukocyte infiltration and TNF-alpha expression. The results suggest that chronic alcohol consumption increased periodontal inflammation, oxidative damage, and TNF-alpha production and had an additive effect on polymorphonuclear leukocyte infiltration and gingival oxidative damage, increasing the severity of periodontal inflammation in the ligature model.
Irie
K
K
Department of Oral Health, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikatacho, Okayama 700-8525, Japan.
Tomofuji
T
T
Tamaki
N
N
Sanbe
T
T
Ekuni
D
D
Azuma
T
T
Maruyama
T
T
Yamamoto
T
T
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000428:Alcohol Drinking / Q000009:adverse effects* / Q000276:immunology / Q000473:pathology; D000818:Animals; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004195:Disease Models, Animal; D000431:Ethanol / Q000494:pharmacology*; D005881:Gingiva / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000097:blood; D008297:Male; D020556:Neutrophil Infiltration / Q000187:drug effects; D010518:Periodontitis / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism; D012720:Severity of Illness Index; D018709:Statistics, Nonparametric; D019237:Tooth Cervix / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D017382:Reactive Oxygen Species; D014409:Tumor Necrosis Factor-alpha; D000431:Ethanol; D000080242:8-Hydroxy-2'-Deoxyguanosine; D006861:Hydrogen Peroxide; D003849:Deoxyguanosine; D005978:Glutathione