[Serial analysis of gene expression in parasitological research].
Serial analysis of gene expression (SAGE) is a powerful high-throughput experimental technique that allows rapid, quantitative analysis of global gene expression in eukaryotic organisms. A short sequence taq (10-14 bp), which is defined by an anchoring enzyme site at a fixed distance from polyA tail, contains sufficient information to identify mRNA transcript from which it originates. The taqs are ligated to obtain concatemers that are cloned into a plasmid vector for sequencing. The identification and abundance of mRNA can be observed through bioinformatics and statistical analysis of a given tag. SAGE is not only applied in obtaining global profile of gene expression in a given cell or tissue, but also help identify a set of specific genes to the cellular conditions by comparing the profiles constructed for a pair of cells that are kept at different conditions. This review covers a general introduction of SAGE, its protocol, methodological evolution and applications in parasite biology.
Li
Qiao-Li
QL
College of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Zhang
Zhi-Ming
ZM
Qiao
Zhong-Dong
ZD
D000818:Animals; D020869:Gene Expression Profiling / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010274:Parasitology / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results
D012333:RNA, Messenger