Is the alkaline tide a signal to activate metabolic or ionoregulatory enzymes in the dogfish shark (Squalus acanthias)?
Experimental metabolic alkalosis is known to stimulate whole-animal urea production and active ion secretion by the rectal gland in the dogfish shark. Furthermore, recent evidence indicates that a marked alkaline tide (systemic metabolic alkalosis) follows feeding in this species and that the activities of the enzymes of the ornithine-urea cycle (OUC) for urea synthesis in skeletal muscle and liver and of energy metabolism and ion transport in the rectal gland are increased at this time. We therefore evaluated whether alkalosis and/or NaCl/volume loading (which also occurs with feeding) could serve as a signal for activation of these enzymes independent of nutrient loading. Fasted dogfish were infused for 20 h with either 500 mmol L(-1) NaHCO3 (alkalosis + volume expansion) or 500 mmol L(-1) NaCl (volume expansion alone), both isosmotic to dogfish plasma, at a rate of 3 mL kg(-1) h(-1). NaHCO3 infusion progressively raised arterial pH to 8.28 (control = 7.85) and plasma [HCO3-] to 20.8 mmol L(-1) (control = 4.5 mmol L(-1)) at 20 h, with unchanged arterial P(CO2), whereas NaCl/volume loading had no effect on blood acid-base status. Rectal gland Na+,K+-ATPase activity was increased 50% by NaCl loading and more than 100% by NaHCO3 loading, indicating stimulatory effects of both volume expansion and alkalosis. Rectal gland lactate dehydrogenase activity was elevated 25% by both treatments, indicating volume expansion effects only, whereas neither treatment increased the activities of the aerobic enzymes citrate synthase, NADP-isocitrate dehydrogenase, or the ketone body-utilizing enzyme beta-hydroxybutyrate dehydrogenase in the rectal gland or liver. The activity of ornithine-citrulline transcarbamoylase in skeletal muscle was doubled by NaHCO3 infusion, but neither treatment altered the activities of other OUC-related enzymes (glutamine synthetase, carbamoylphosphate synthetase III). We conclude that both the alkaline tide and salt loading/volume expansion act as signals to activate some but not all of the elevated metabolic pathways and ionoregulatory mechanisms needed during processing of a meal.
Wood
Chris M
CM
Department of Biology, McMaster University, 1280 Main Street West, Hamilton, Ontario L8S 4K1, Canada. woodcm@mcmaster.ca
Kajimura
Makiko
M
Mommsen
Thomas P
TP
Walsh
Patrick J
PJ
D000471:Alkalosis / Q000378:metabolism*; D000818:Animals; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology*; D029941:Fish Proteins / Q000378:metabolism; D005508:Food Deprivation; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000097:blood / Q000378:metabolism*; D008297:Male; D012623:Seawater / Q000737:chemistry; D017693:Sodium Bicarbonate / Q000097:blood / Q000494:pharmacology; D012965:Sodium Chloride / Q000097:blood / Q000494:pharmacology; D000254:Sodium-Potassium-Exchanging ATPase / Q000097:blood / Q000378:metabolism*; D048251:Squalus acanthias / Q000097:blood / Q000502:physiology*; D014882:Water-Electrolyte Balance
D029941:Fish Proteins; D012965:Sodium Chloride; D017693:Sodium Bicarbonate; D007770:L-Lactate Dehydrogenase; D000254:Sodium-Potassium-Exchanging ATPase