Gene amplification of the multidrug resistance 1 gene of Plasmodium vivax isolates from Thailand, Laos, and Myanmar.
Plasmodium vivax mdr1 gene amplification, quantified by real-time PCR, was significantly more common on the western Thailand border (6 of 66 samples), where mefloquine pressure has been intense, than elsewhere in southeast Asia (3 of 149; P = 0.02). Five coding mutations in pvmdr1, independent of gene amplification, were also found.
Imwong
Mallika
M
Department of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Rd., Bangkok 10400, Thailand. noi@tropmedres.ac
Pukrittayakamee
Sasithon
S
Pongtavornpinyo
Wirichada
W
Nakeesathit
Supatchara
S
Nair
Shalini
S
Newton
Paul
P
Nosten
Francois
F
Anderson
Timothy J C
TJ
Dondorp
Arjen
A
Day
Nicholas P J
NP
White
Nicholas J
NJ
D000818:Animals; D000962:Antimalarials / Q000494:pharmacology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D018432:Drug Resistance, Multiple / Q000235:genetics; D005784:Gene Amplification; D018628:Gene Dosage; D019450:Genes, MDR; D017125:Genes, Protozoan; D006801:Humans; D007812:Laos; D016780:Malaria, Vivax / Q000188:drug therapy / Q000469:parasitology; D002052:Myanmar; D010966:Plasmodium vivax / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D013785:Thailand
D000962:Antimalarials; D017931:DNA Primers; D016054:DNA, Protozoan