Novel composite hyaluronan/type I collagen/fibrin scaffold enhances repair of osteochondral defect in rabbit knee.
A new composite scaffold containing type I collagen, hyaluronan, and fibrin was prepared with and without autologous chondrocytes and implanted into a rabbit femoral trochlea. The biophysical properties of the composite scaffold were similar to native cartilage. The macroscopic, histological, and immunohistochemical analysis of the regenerated tissue from cell-seeded scaffolds was performed 6 weeks after the implantation and predominantly showed formation of hyaline cartilage accompanied by production of glycosaminoglycans and type II collagen with minor fibro-cartilage production. Implanted scaffolds without cells healed predominantly as fibro-cartilage, although glycosaminoglycans and type II collagen, which form hyaline cartilage, were also observed. On the other hand, fibro-cartilage or fibrous tissue or both were only formed in the defects without scaffold. The new composite scaffold containing collagen type I, hyaluronan, and fibrin, seeded with autologous chondrocytes and implanted into rabbit femoral trochlea, was found to be highly effective in cartilage repair after only 6 weeks. The new composite scaffold can therefore enhance cartilage regeneration of osteochondral defects, by the supporting of the hyaline cartilage formation.
Filová
Eva
E
Department of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic. evafil@biomed.cas.cz
Jelínek
Frantisek
F
Handl
Milan
M
Lytvynets
Andriy
A
Rampichová
Michala
M
Varga
Ferdinand
F
Cinátl
Jaroslav
J
Soukup
Tomás
T
Trc
Tomás
T
Amler
Evzen
E
D000818:Animals; D001696:Biomechanical Phenomena; D002470:Cell Survival; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D024042:Collagen Type I; D005337:Fibrin; D006820:Hyaluronic Acid; D007718:Knee Injuries / Q000601:surgery*; D008297:Male; D008422:Materials Testing; D008854:Microscopy, Electron; D019736:Prostheses and Implants; D011817:Rabbits
D024042:Collagen Type I; D005337:Fibrin; D006820:Hyaluronic Acid