Preservation of non-heart-beating donor livers in extracorporeal liver perfusion and histidine-trytophan-ketoglutarate solution.
OBJECTIVE
To compare the preservation of non-heart-beating donor (NHBD) livers in cold histidine-trytophan-ketoglutarate (HTK) solution and extracorporeal liver perfusion (ECLP).


METHODS
Livers harvested from healthy pigs were stored for 10 h in cold HTK solution (group A, n = 4) or perfused with oxygenated autologous blood at body temperature (group B, n = 4). Both groups were then tested on the circuit for 4 h. Bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of extracorporeal livers were tested in each group. Liver tissues from each group were examined at the end of reperfusion.


RESULTS
At 1, 2, 3 and 4 h after reperfusion, bile production, hemodynamic parameters, hepatocyte markers and reperfusion injury of livers in group A were statistically different from those in group B (P < 0.05 or P < 0.01).


CONCLUSIONS
ECLP is better than HTK solution to preserve NHBD livers. ECLP can assess the graft viability before liver transplantation.
Gong
Jin
J
Department of Surgery, The First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China. gongjin622@yahoo.com.cn
Lao
Xue-Jun
XJ
Wang
Xi-Mo
XM
Long
Gang
G
Jiang
Tao
T
Chen
Shi
S
D000818:Animals; D001646:Bile / Q000378:metabolism; D003080:Cold Temperature; D005112:Extracorporeal Circulation / Q000009:adverse effects; D005947:Glucose / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology; D006439:Hemodynamics / Q000187:drug effects; D008099:Liver / Q000098:blood supply / Q000187:drug effects* / Q000378:metabolism / Q000601:surgery* / Q000648:ultrastructure; D008102:Liver Circulation / Q000187:drug effects; D016031:Liver Transplantation; D008353:Mannitol / Q000009:adverse effects / Q000494:pharmacology; D009926:Organ Preservation / Q000009:adverse effects / Q000379:methods*; D019188:Organ Preservation Solutions / Q000009:adverse effects / Q000494:pharmacology*; D010101:Oxygen Consumption / Q000187:drug effects; D011189:Potassium Chloride / Q000009:adverse effects / Q000494:pharmacology; D011343:Procaine / Q000009:adverse effects / Q000494:pharmacology; D015424:Reperfusion / Q000009:adverse effects; D015427:Reperfusion Injury / Q000209:etiology / Q000517:prevention & control; D013552:Swine; D013997:Time Factors
C039984:Bretschneider cardioplegic solution; D019188:Organ Preservation Solutions; D008353:Mannitol; D011343:Procaine; D011189:Potassium Chloride; D005947:Glucose