title: Gold nanoparticles for one step DNA extraction and real-time PCR of pathogens in a single chamber.
abstract: The optothermal properties of nanoparticles are of interest for biosensors and highly sensitive biochip applications. In this respect, the longitudinal resonance of Au nanorods was used to transform near infrared energy into thermal energy in a microfluidic chip. The resulting heat generated effectively caused pathogen lysis. Consequently the DNA was extracted out of the cell body and transferred to a PCR system. This resulted in the successful demonstration of a one step real-time PCR system for pathogen detection without removal or changing of reagents.
authors.affiliation: Bio & Health Lab, Samsung Advanced Institute of Technology, Giheung, Korea. kwangho.cheong@samsung.com
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Cheong
authors.forename: Dong Kee
authors.initials: DK
authors.lastname: Yi
authors.forename: Jeong-Gun
authors.initials: JG
authors.lastname: Lee
authors.forename: Jong-Myeon
authors.initials: JM
authors.lastname: Park
authors.forename: Min Jun
authors.initials: MJ
authors.lastname: Kim
authors.forename: Joshua B
authors.initials: JB
authors.lastname: Edel
authors.forename: Christopher
authors.initials: C
authors.lastname: Ko
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D004926:Escherichia coli / Q000737:chemistry / Q000235:genetics; D006046:Gold / Q000737:chemistry*; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D046208:Microchip Analytical Procedures / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D004269:DNA, Bacterial; D006046:Gold