Use of protein folding reagents.
The reagents and methods for purification of the denaturants guanidine hydrochloride (guanidine-HCl) and urea are described. Sulfhydryl reagents (reducing agents) and "oxido-shuffling" (or oxidative regeneration) systems are also discussed.
Wingfield
P T
PT
National Institutes of Health, Bethesda, Maryland, USA.
D004229:Dithiothreitol; D005978:Glutathione; D019791:Guanidine / Q000302:isolation & purification*; D008623:Mercaptoethanol; D016877:Oxidants; D011489:Protein Denaturation; D017510:Protein Folding; D019163:Reducing Agents; D013439:Sulfhydryl Reagents; D014508:Urea / Q000302:isolation & purification*
D016877:Oxidants; D019163:Reducing Agents; D013439:Sulfhydryl Reagents; D008623:Mercaptoethanol; D014508:Urea; D005978:Glutathione; D019791:Guanidine; D004229:Dithiothreitol