The effects of nitronium ion on nitration, carbonylation and coagulation of human fibrinogen.
The effect of nitronium ion on nitration, carbonylation and coagulation of human fibrinogen (Fg) in vitro was investigated. We observed that nitration of tyrosine, induced by NO2BF4 (0.01 mmol/l), was increased. No changes in carbonylation by NO2BF4 (0.01 mmol/l) were noticed. Mentioned alterations were associated with amplified coagulation of Fg. Higher concentrations of NO2BF4 (1 and 0.1 mmol/l) triggered growth of nitration and carbonylation of Fg, but led to inhibition of polymerization. Slight nitration may be responsible for increase, whereas sizable nitration and oxidation may lead to inhibition of Fg coagulation.
Ponczek
M B
MB
Department of General Biochemistry, University of Lodz, Banacha 12/16, 90 273 Lodz, Poland. mponczek@biol.uni.lodz.pl
Nowak
P
P
Wachowicz
B
B
D001777:Blood Coagulation / Q000187:drug effects; D005340:Fibrinogen / Q000737:chemistry* / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D046912:Multiprotein Complexes / Q000737:chemistry / Q000187:drug effects; D009566:Nitrates / Q000737:chemistry; D009585:Nitrogen Dioxide / Q000494:pharmacology; D050050:Protein Carbonylation / Q000187:drug effects; D026361:Reactive Nitrogen Species / Q000494:pharmacology*
D046912:Multiprotein Complexes; D009566:Nitrates; D026361:Reactive Nitrogen Species; D005340:Fibrinogen; D009585:Nitrogen Dioxide