Co-culture of arbuscular mycorrhiza-like fungi (Piriformospora indica and Sebacina vermifera) with plant cells of Linum album for enhanced production of podophyllotoxins: a first report.
Cell suspension cultures of Linum album were developed from internode portions of in vitro germinated plant in Gamborg's B5 medium supplemented with 0.4 mg naphthalene acetic acid/l. The highest biomass was 8.5 g/l with podophyllotoxin and 6-methoxypodophyllotoxin at 29 and 1.9 mg/l, respectively after 12 d cultivation. Co-cultures of L. album cells with axenically cultivable arbuscular mycorrhiza-like fungi, Piriformospora indica and Sebacina vermifera, were established for the first time. These enhanced podophyllotoxin and 6-methoxypodophyllotoxin production by about four- and eight-fold, respectively, along with a 20% increase in biomass compared to the control cultures.
Baldi
Ashish
A
Plant Cell Culture Laboratory, Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110 016, India.
Jain
Abhishek
A
Gupta
Nishant
N
Srivastava
A K
AK
Bisaria
V S
VS
D018920:Coculture Techniques; D019597:Flax / Q000166:cytology* / Q000201:enzymology / Q000254:growth & development; D007700:Kinetics; D038821:Mycorrhizae / Q000166:cytology*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D011034:Podophyllotoxin / Q000031:analogs & derivatives / Q000096:biosynthesis* / Q000378:metabolism
C502514:6-methoxypodophyllotoxin; D010650:Phenylalanine Ammonia-Lyase; D011034:Podophyllotoxin