Low level laser therapy (LLLT) decreases pulmonary microvascular leakage, neutrophil influx and IL-1beta levels in airway and lung from rat subjected to LPS-induced inflammation.
OBJECTIVE
Low level laser therapy (LLLT) is a known anti-inflammatory therapy. Herein we studied the effect of LLLT on lung permeability and the IL-1beta level in LPS-induced pulmonary inflammation. STUDY DESIGN/METHODOLOGY: Rats were divided into 12 groups (n = 7 for each group). Lung permeability was measured by quantifying extravasated albumin concentration in lung homogenate, inflammatory cells influx was determined by myeloperoxidase activity, IL-1beta in BAL was determined by ELISA and IL-1beta mRNA expression in trachea was evaluated by RT-PCR. The rats were irradiated on the skin over the upper bronchus at the site of tracheotomy after LPS.


RESULTS
LLLT attenuated lung permeability. In addition, there was reduced neutrophil influx, myeloperoxidase activity and both IL-1beta in BAL and IL-1beta mRNA expression in trachea obtained from animals subjected to LPS-induced inflammation.


CONCLUSIONS
LLLT reduced the lung permeability by a mechanism in which the IL-1beta seems to have an important role.
Aimbire
F
F
Instituto de Pesquisa & Desenvolvimento-IP&D, Universidade do Vale do Paraíba-UNIVAP, Av. Shishima Hifumi, 2911, CEP: 12244-000, São José dos Campos, São Paulo, Brazil. aimbire@univap.br
Ligeiro de Oliveira
A P
AP
Albertini
R
R
Corrêa
J C
JC
Ladeira de Campos
C B
CB
Lyon
J P
JP
Silva
J A
JA
Costa
M S
MS
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology; D002199:Capillary Permeability / Q000187:drug effects / Q000528:radiation effects*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D053583:Interleukin-1beta / Q000235:genetics / Q000378:metabolism*; D008070:Lipopolysaccharides; D028022:Low-Level Light Therapy; D008168:Lung / Q000098:blood supply / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D008297:Male; D020556:Neutrophil Infiltration / Q000187:drug effects / Q000528:radiation effects*; D009504:Neutrophils / Q000187:drug effects / Q000201:enzymology / Q000528:radiation effects*; D009195:Peroxidase / Q000378:metabolism; D011014:Pneumonia / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism / Q000532:radiotherapy*; D016133:Polymerase Chain Reaction; D011506:Proteins / Q000494:pharmacology; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D018123:Receptors, Interleukin / Q000037:antagonists & inhibitors / Q000378:metabolism; D013997:Time Factors; D014132:Trachea / Q000187:drug effects / Q000276:immunology / Q000528:radiation effects*; D014140:Tracheotomy
C102533:AF 12198; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; D011506:Proteins; D012333:RNA, Messenger; D018123:Receptors, Interleukin; D009195:Peroxidase