title: Synthesis of oligoribonucleotides using the 2-nitrobenzyloxymethyl group for 2'-hydroxyl protection.
abstract: RNA can be rapidly synthesized using protected ribonucleoside phosphoramidite monomers that carry 2-nitrobenzyloxymethyl groups on their 2-hydroxyls. These protecting groups are removed from the oligoribonucleotide products by exposure to long-wave UV light.
authors.affiliation: Purdue University, West Lafayette, Indiana, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Miller
authors.forename: M E
authors.initials: ME
authors.lastname: Schwartz
authors.forename: G R
authors.initials: GR
authors.lastname: Gough
mesh_terms: D001593:Benzyl Compounds / Q000737:chemistry*; D001671:Biochemistry / Q000379:methods*; D017665:Hydroxyl Radical / Q000737:chemistry*; D009578:Nitrobenzenes / Q000737:chemistry*; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D009943:Organophosphorus Compounds / Q000138:chemical synthesis / Q000737:chemistry / Q000302:isolation & purification; D012263:Ribonucleosides / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: C528724:2-nitrobenzyloxymethyl; D001593:Benzyl Compounds; D009578:Nitrobenzenes; D009843:Oligoribonucleotides; D009943:Organophosphorus Compounds; D012263:Ribonucleosides; C434331:phosphoramidite; D017665:Hydroxyl Radical