Regional metabolite T2 in the healthy rhesus macaque brain at 7T.
Although the rhesus macaque brain is an excellent model system for the study of neurological diseases and their responses to treatment, its small size requires much higher spatial resolution, motivating use of ultra-high-field (B(0)) imagers. Their weaker radio-frequency fields, however, dictate longer pulses; hence longer TE localization sequences. Due to the shorter transverse relaxation time (T(2)) at higher B(0)s, these longer TEs subject metabolites to T(2)-weighting, that decrease their quantification accuracy. To address this we measured the T(2)s of N-acetylaspartate (NAA), choline (Cho), and creatine (Cr) in several gray matter (GM) and white matter (WM) regions of four healthy rhesus macaques at 7T using three-dimensional (3D) proton MR spectroscopic imaging at (0.4 cm)(3) = 64 mul spatial resolution. The results show that macaque T(2)s are in good agreement with those reported in humans at 7T: 169 +/- 2.3 ms for NAA (mean +/- SEM), 114 +/- 1.9 ms for Cr, and 128 +/- 2.4 ms for Cho, with no significant differences between GM and WM. The T(2) histograms from 320 voxels in each animal for NAA, Cr, and Cho were similar in position and shape, indicating that they are potentially characteristic of "healthy" in this species.
Liu
Songtao
S
Department of Radiology, New York University School of Medicine, New York, New York, USA.
Gonen
Oded
O
Fleysher
Lazar
L
Fleysher
Roman
R
Soher
Brian J
BJ
Pilkenton
Sarah
S
Lentz
Margaret R
MR
Ratai
Eva-Maria
EM
González
R Gilberto
RG
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D001931:Brain Mapping / Q000379:methods*; D002794:Choline / Q000378:metabolism; D003401:Creatine / Q000378:metabolism; D005260:Female; D007091:Image Processing, Computer-Assisted; D008253:Macaca mulatta; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male
D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine; D002794:Choline