title: Genomic characterization of a novel virus found in papillomatous lesions from a southern brown bandicoot (Isoodon obesulus) in Western Australia.
abstract: The genome of a novel virus, tentatively named bandicoot papillomatosis carcinomatosis virus type 2 (BPCV2), obtained from multicentric papillomatous lesions from an adult male southern brown bandicoot (Isoodon obesulus) was sequenced in its entirety. BPCV2 had a circular double-stranded DNA genome consisting of 7277 bp and open reading frames encoding putative L1 and L2 structural proteins and putative large T antigen and small t antigen transforming proteins. These genomic features, intermediate between Papillomaviridae and Polyomaviridae are most similar to BPCV1, recently described from papillomas and carcinomas in the endangered western barred bandicoot (Perameles bougainville). This study also employed in situ hybridization to definitively demonstrate BPCV2 DNA within lesion biopsies. The discovery of BPCV2 provides evidence of virus-host co-speciation between BPCVs and marsupial bandicoots and has important implications for the phylogeny and taxonomy of circular double-stranded DNA viruses infecting vertebrates.
authors.affiliation: School of Veterinary and Biomedical Sciences, Murdoch University, Perth, Western Australia, 6150, Australia. m.bennet@murdoch.edu.au
authors.forename: Mark D
authors.initials: MD
authors.lastname: Bennett
authors.forename: Lucy
authors.initials: L
authors.lastname: Woolford
authors.forename: Hans
authors.initials: H
authors.lastname: Stevens
authors.forename: Marc
authors.initials: M
authors.lastname: Van Ranst
authors.forename: Timothy
authors.initials: T
authors.lastname: Oldfield
authors.forename: Michael
authors.initials: M
authors.lastname: Slaven
authors.forename: Amanda J
authors.initials: AJ
authors.lastname: O'Hara
authors.forename: Kristin S
authors.initials: KS
authors.lastname: Warren
authors.forename: Philip K
authors.initials: PK
authors.lastname: Nicholls
mesh_terms: D000818:Animals; D004270:DNA, Circular / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D016679:Genome, Viral; D017403:In Situ Hybridization; D008297:Male; D008394:Marsupialia / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010212:Papilloma / Q000821:virology*; D027383:Papillomaviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D027382:Polyomaviridae / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000235:genetics; D014904:Western Australia
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral; D014764:Viral Proteins