Optimization of transfection.
The single most important factor in optimizing transfection efficiency is selecting the proper transfection protocol. This overview discusses the most commonly used procedures: calcium phosphate-mediated gene transfer, DEAE-dextran-mediated gene transfer, electroporation, and liposome-mediated transfection. Fusion techniques such as protoplast fusion and microinjection may also be considered.
Rose
J K
JK
Yale University School of Medicine, New Haven, Connecticut, USA.
D000818:Animals; D002130:Calcium Phosphates / Q000008:administration & dosage; D002478:Cells, Cultured; D003637:DEAE-Dextran / Q000008:administration & dosage; D004247:DNA / Q000008:administration & dosage; D018274:Electroporation; D008081:Liposomes / Q000008:administration & dosage; D014162:Transfection / Q000379:methods*
D002130:Calcium Phosphates; D008081:Liposomes; D004247:DNA; D003637:DEAE-Dextran; C020243:calcium phosphate