Antibiofilm activity of sodium bicarbonate, sodium metaperiodate and SDS combination against dental unit waterline-associated bacteria and yeast.
OBJECTIVE
To determine the effect of sodium bicarbonate (SB), sodium metaperiodate (SMP) and sodium dodecyl sulfate (SDS) combination on biofilm formation and dispersal in dental unit waterline (DUWL)-associated bacteria and yeast.


RESULTS
The in vitro effect of SB, SMP and SDS alone and in combination on biofilm formation and dispersal in Pseudomonas aeruginosa, Klebsiella pneumoniae, Actinomyces naeslundii, and Candida albicans was investigated using a 96-well microtitre plate biofilm assay. The combination showed a broad-spectrum inhibitory effect on growth as well as biofilm formation of both gram-negative and gram-positive bacteria, and yeast. In addition, the SB + SMP + SDS combination was significantly more effective in dispersing biofilm than the individual compounds. The combination dispersed more than 90% of P. aeruginosa biofilm whereas the commercial products, Oxygenal 6, Sterilex Ultra, and PeraSafe showed no biofilm dispersal activity.


CONCLUSIONS
The composition comprising SB, SMP, and SDS was effective in inhibiting as well as dispersing biofilms in DUWL-associated bacteria and yeast.


CONCLUSIONS
This study shows that a composition comprising environmentally friendly and biologically safe compounds such as SB, SMP, and SDS has a potential application in reducing DUWL-associated acquired infections in dental clinics.
Gawande
P V
PV
Kane Biotech Inc., Winnipeg, MB, Canada.
LoVetri
K
K
Yakandawala
N
N
Romeo
T
T
Zhanel
G G
GG
Cvitkovitch
D G
DG
Madhyastha
S
S
D001419:Bacteria / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects*; D019551:Dental Disinfectants / Q000494:pharmacology*; D004203:Disinfection / Q000379:methods*; D006801:Humans; D019552:Infection Control, Dental / Q000379:methods*; D008826:Microbial Sensitivity Tests; D008934:Mitogens / Q000494:pharmacology; D010504:Periodic Acid / Q000494:pharmacology; D017693:Sodium Bicarbonate / Q000494:pharmacology; D012967:Sodium Dodecyl Sulfate / Q000494:pharmacology; D013501:Surface-Active Agents / Q000494:pharmacology; D014871:Water Microbiology; D015003:Yeasts / Q000187:drug effects
D019551:Dental Disinfectants; D008934:Mitogens; D013501:Surface-Active Agents; D010504:Periodic Acid; D012967:Sodium Dodecyl Sulfate; D017693:Sodium Bicarbonate; C009288:metaperiodate