Immunomagnetic separation combined with real-time reverse transcriptase PCR assays for detection of norovirus in contaminated food.
We developed an immunomagnetic separation (IMS) technique combined with real-time TaqMan reverse transcriptase PCR (RT-PCR), which allowed detection of norovirus at a level as low as 3 to 7 RT-PCR units from artificially contaminated strawberries. The inoculum recovery rate ranged from 14 to 30%. The data demonstrate that IMS combined with real-time RT-PCR will be useful as a rapid and sensitive method for detecting food-borne microbial contaminants.
Park
YoungBin
Y
Department of Environmental Health, School of Public Health, Seoul National University, 28 Yunkeun-Dong, Chongro-Ku, Seoul 110-799, South Korea.
Cho
You-Hee
YH
Jee
YoungMee
Y
Ko
GwangPyo
G
D005506:Food Contamination; D031985:Fragaria / Q000382:microbiology*; D018189:Immunomagnetic Separation / Q000379:methods*; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase; D013997:Time Factors
D019914:Taq Polymerase