Effect of amino acids and amino acid derivatives on crystallization of hemoglobin and ribonuclease A.
Determination of the appropriate conditions for protein crystallization remains a highly empirical process. Preventing protein aggregation is necessary for the formation of single crystals under aggregation-prone solution conditions. Because many amino acids and amino acid derivatives offer a unique combination of solubility and stabilizing properties, they open new avenues into the field of protein aggregation research. The use of amino acids and amino acid derivatives can potentially influence processes such as heat treatment and refolding reactions. The effect of the addition of several amino acids, such as lysine, and several amino acid derivatives, such as glycine ethyl ester and glycine amide, on the crystallization of equine hemoglobin and bovine pancreatic ribonuclease A has been examined. The addition of these amino acids and amino acid derivatives expanded the range of precipitant concentration in which crystals formed without aggregation. The addition of such additives appears to promote the crystallization of proteins.
Ito
Len
L
School of Science and Technology, Kwansei Gakuin University, 2-1 Gakuen, Sanda, Hyogo 669-1337, Japan. len@ksc.kwansei.ac.jp
Kobayashi
Toyoaki
T
Shiraki
Kentaro
K
Yamaguchi
Hiroshi
H
D000596:Amino Acids / Q000737:chemistry*; D003460:Crystallization; D006454:Hemoglobins / Q000737:chemistry*; D012259:Ribonuclease, Pancreatic / Q000737:chemistry*
D000596:Amino Acids; D006454:Hemoglobins; D012259:Ribonuclease, Pancreatic