title: Animal models for the analysis of immune responses to leishmaniasis.
abstract: This unit focuses on the murine model of cutaneous leishmaniasis and models of visceral leishmaniasis in mice and hamsters. Each basic protocol describes the methods used to inoculate parasites and to evaluate infections with regard to lesion progression and visceralization, and quantification of parasite load. Five support protocols are provided; two for the growth and isolation of metacyclic promastigotes from in vitro culture, one for isolation of tissue amastigotes from infected animals, one for cryopreservation of parasites, and one for the preparation of blood agar plates for quantitation of parasite numbers in infected tissue.
authors.affiliation: National Institute of Allergy & Infectious, Diseases, Bethesda, Maryland, USA.
authors.forename: D L
authors.initials: DL
authors.lastname: Sacks
authors.forename: P C
authors.initials: PC
authors.lastname: Melby
mesh_terms: D000818:Animals; D003470:Culture Media; D004195:Disease Models, Animal; D004912:Erythrocytes; D007891:Leishmania / Q000254:growth & development / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology*; D008018:Life Cycle Stages; D051379:Mice
chemical_list: D003470:Culture Media