Clinicopathological significance of the gene expression of matrix metalloproteinases and reversion-inducing cysteine-rich protein with Kazal motifs in patients with colorectal cancer: MMP-2 gene expression is a useful predictor of liver metastasis from colorectal cancer.
Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and membrane-type matrix metalloproteinase 1 (MT1-MMP) are involved in colorectal cancer invasion and metastasis. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) inhibits MMP-2, MMP-9 and MT1-MMP. We examined the clinicopathological significance of the relative expression of these genes in patients with colorectal cancer, especially with regard to metastasis. We studied surgical specimens of cancer tissue and adjacent normal mucosa obtained from 205 patients with untreated colorectal carcinoma. MMP-2, MMP-9, MT1-MMP, RECK and beta-actin mRNA of cancer tissue and adjacent normal mucosa were measured by quantitative real-time reverse-transcriptase polymerase chain reaction. MT1-MMP gene expression was higher in cancer tissue than in adjacent normal mucosa. In contrast, MMP-2, MMP-9 and RECK gene expression levels were lower in cancer tissue than in adjacent normal mucosa. As for the relationship between the gene expression and clinicopathological factors, MMP-2 expression correlated with the depth of invasion, venous invasion and liver metastasis; MMP-9 and RECK expression correlated with venous invasion. There were positive correlations among the gene expression levels of MMP-2, MMP-9 and RECK. MMP-2 gene expression was considered a useful predictor of liver metastasis from colorectal cancer.
Oshima
Takashi
T
Gastroenterological Center, Yokohama City University Medical Center, Kanagawa-ken 232-0024, Japan. ohshimatakashi@yahoo.co.jp
Kunisaki
Chikara
C
Yoshihara
Kazue
K
Yamada
Roppei
R
Yamamoto
Naoto
N
Sato
Tsutomu
T
Makino
Hirochika
H
Yamagishi
Shigeru
S
Nagano
Yasuhiko
Y
Fujii
Shoich
S
Shiozawa
Manabu
M
Akaike
Makoto
M
Wada
Nobuyuki
N
Rino
Yasushi
Y
Masuda
Munetaka
M
Tanaka
Katsuaki
K
Imada
Toshio
T
D000368:Aged; D020816:Amino Acid Motifs; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D058851:GPI-Linked Proteins; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008024:Ligands; D008113:Liver Neoplasms / Q000378:metabolism* / Q000556:secondary*; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis*; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis*; D020782:Matrix Metalloproteinases / Q000096:biosynthesis*; D008562:Membrane Glycoproteins / Q000096:biosynthesis*; D008875:Middle Aged; D009362:Neoplasm Metastasis
D058851:GPI-Linked Proteins; D008024:Ligands; D008562:Membrane Glycoproteins; C115460:RECK protein, human; D020782:Matrix Metalloproteinases; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9