Specific monoclonal antibodies raised against Taura syndrome virus (TSV) capsid protein VP3 detect TSV in single and dual infections with white spot syndrome virus (WSSV).
The gene sequence encoding VP3 capsid protein of Taura syndrome virus (TSV) was cloned into pGEX-6P-1 expression vector and transformed into Escherichia coli BL21. After induction, recombinant GST-VP3 (rVP3) fusion protein was obtained and further purified by electro-elution before use in immunizing Swiss mice for production of monoclonal antibodies (MAb). One MAb specific to glutathione-S-transferase (GST) and 6 MAb specific to VP3 were selected using dot blotting and Western blotting. MAb specific to VP3 could be used to detect natural TSV infections in farmed whiteleg shrimp Penaeus vannamei by dot blotting and Western blotting, without cross reaction to shrimp tissues or other shrimp viruses, such as white spot syndrome virus (WSSV), yellow head virus (YHV), monodon baculovirus (MBV) and hepatopancreatic parvovirus (HPV). These MAb were also used together with those specific for WSSV to successfully detect TSV and WSSV in dual infections in farmed P. vannamei.
Longyant
Siwaporn
S
Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
Poyoi
Piengjan
P
Chaivisuthangkura
Parin
P
Tejangkura
Thanawan
T
Sithigorngul
Weerawan
W
Sithigorngul
Paisarn
P
Rukpratanporn
Sombat
S
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology*; D004926:Escherichia coli / Q000235:genetics; D051379:Mice; D033561:Penaeidae / Q000821:virology*; D010849:Picornaviridae / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000276:immunology; D015203:Reproducibility of Results; D046848:White spot syndrome virus 1 / Q000302:isolation & purification*
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins