title: Effect of BCG stimulus on proinflammatory cytokine production in laryngeal cancer.
abstract: BACKGROUND
  Evaluate the production of TNF and IL-6 in the supernatant of peripheral blood mononuclear cell (PBMC) cultures of patients with supraglottic laryngeal cancer before and after surgical treatment.
  
  
  METHODS
  Adherent cell cultures were stimulated with LPS and BCG. Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cytokine concentration was determined by ELISA in supernatants of mononuclear cell cultures.
  
  
  RESULTS
  In non-stimulated cultures, lower TNF cytokine levels were detected during the late postoperative (LP) period compared to control (P = 0.02). LP TNF and IL-6 levels were high in cultures stimulated with LPS compared with the preoperative period (PREOP) (P = 0.007; P = 0.008, respectively). Stimulation with BCG led to increased levels of TNF and IL-6 during the LP period compared to control (P = 0.001; P = 0.04, respectively).
  
  
  CONCLUSIONS
  BCG is able to modulate the immune response of patients with advanced supraglottic laryngeal cancer in vitro, increasing the secretion of TNF and IL-6 by macrophages during the postoperative period.
authors.affiliation: Department of Head and Neck Surgery, Ribeirao Preto Medical School, University of Sao Paulo, Ribeirao Preto, Brazil. lcconti@uol.com.br
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: Conti-Freitas
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Foss-Freitas
authors.forename: Rui Celso Martins
authors.initials: RC
authors.lastname: Mamede
authors.forename: Norma Tiraboschi
authors.initials: NT
authors.lastname: Foss
mesh_terms: D000328:Adult; D000368:Aged; D001500:BCG Vaccine / Q000276:immunology / Q000627:therapeutic use*; D002277:Carcinoma / Q000188:drug therapy*; D016207:Cytokines / Q000096:biosynthesis* / Q000097:blood / Q000276:immunology; D005260:Female; D006801:Humans; D007249:Inflammation; D015850:Interleukin-6 / Q000096:biosynthesis / Q000097:blood / Q000276:immunology; D007822:Laryngeal Neoplasms / Q000188:drug therapy* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D012907:Smoking; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis / Q000097:blood / Q000276:immunology
chemical_list: D001500:BCG Vaccine; D016207:Cytokines; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha