title: Antioxidant activity of vinegar produced from distilled residues of the Japanese liquor shochu.
abstract: Rice shochu distilled residue (RSDR) is a byproduct of rice shochu production. RSDR was converted into vinegar by acetate fermentation. In our present study, two major antioxidant compounds, tyrosol and ferulic acid, were identified from the RSDR-derived vinegar. Furthermore, we investigated the antioxidant activity of freeze-dried RSDR-derived vinegar, which was Acetobactor aceti fermentation powder (AFP), in vitro and in vivo. AFP at 0.25 mg/mL or higher concentrations showed an inhibitory effect against lipid peroxidation and cellular GSH depletion in HepG2 cells induced by H(2)O(2) (P < 0.05). We thus considered the potential of AFP in protecting cells against damage induced by H(2)O(2). Its antioxidant activity was evaluated in vivo using carbon tetrachloride (CCl(4))-induced acute liver injury mouse models. Five consecutive days of oral preadministration of AFP dissolved in PBS at 200, 400, and 800 mg/kg body weight significantly suppressed lipid peroxidation in the liver induced by CCl(4) (P < 0.01). Consequently, treatment with AFP at 200 mg/kg body weight or higher doses suppressed the elevation of alanine aminotransferase and aspartate aminotransferase levels in serum (P < 0.05). These findings suggest that RSDR-derived vinegar can be developed as a health food with an antioxidant effect for the prevention of oxidative injury and cancer.
authors.affiliation: Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Seki
authors.forename: Shigeru
authors.initials: S
authors.lastname: Morimura
authors.forename: Sachiko
authors.initials: S
authors.lastname: Tabata
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Tang
authors.forename: Toru
authors.initials: T
authors.lastname: Shigematsu
authors.forename: Kenji
authors.initials: K
authors.lastname: Kida
mesh_terms: D019342:Acetic Acid / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D000434:Alcoholic Beverages; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D002251:Carbon Tetrachloride; D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D056486:Chemical and Drug Induced Liver Injury; D003373:Coumaric Acids / Q000032:analysis; D005612:Freeze Drying; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008107:Liver Diseases / Q000517:prevention & control; D008113:Liver Neoplasms; D051379:Mice; D012275:Oryza; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D000975:Antioxidants; D003373:Coumaric Acids; C011867:4-hydroxyphenylethanol; C004999:ferulic acid; D006861:Hydrogen Peroxide; D002251:Carbon Tetrachloride; D010626:Phenylethyl Alcohol; D019342:Acetic Acid