title: Rapid solution application methods.
abstract: Solution changes to deliver solutes of different compositions are required in virtually every cellular electrophysiological experiment. Also, in many neurobiology experiments, it is necessary to make rapid step changes in the concentration of a test compound in order to outpace receptor desensitization or to mimic the brief lifetime of a fast synaptic response. The goal of this unit is to aid the investigator in choosing the rapid solution application method that is most appropriate for the experimental situation and to describe the methods for fabricating two relatively simple devices for making rapid changes between different solutions.
authors.affiliation: University of Maryland School of Medicine, Baltimore, Maryland, USA.
authors.forename: C M
authors.initials: CM
authors.lastname: Tang
mesh_terms: D000818:Animals; D004594:Electrophysiology / Q000295:instrumentation / Q000379:methods; D007478:Iontophoresis / Q000295:instrumentation / Q000379:methods; D008564:Membrane Potentials / Q000502:physiology; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods*; D012996:Solutions / Q000008:administration & dosage* / Q000493:pharmacokinetics; D013997:Time Factors
chemical_list: D012996:Solutions