Intra-individual and inter-individual variations in sperm aneuploidy frequencies in normal men.
OBJECTIVE
To investigate whether there are intra-individual and/or inter-individual variations in sperm aneuploidy frequencies within the normal male population, and, if this is the case, whether they are sporadic or time-stable variants.


METHODS
Prospective study.


METHODS
University research laboratory.


METHODS
Ten men aged 18-32 years.


METHODS
None.


METHODS
Fluorescence in situ hybridization was used to investigate sperm aneuploidy frequencies for chromosomes X, Y, 13, and 21 in serial semen samples collected over a period of 12-18 months.


RESULTS
Intra-individual and inter-individual variations were investigated by comparing serial samples from the same donor and by comparing the donors with each other, respectively. Intra-individual variations were found in all 10 donors for at least one investigated chromosome; variations tended to be sporadic events affecting only one time point. Inter-individual variations were found for all chromosomes (except XX and YY disomy and disomy 21), with three men identified as stable variants, consistently producing higher levels of aneuploidy for at least one of the following aneuploidies: sex chromosome nullisomy; disomy 13, or diploidy.


CONCLUSIONS
These results suggest that there are a number of factors and mechanisms that have the potential to sporadically or consistently affect sperm aneuploidy.
Tempest
Helen G
HG
Department of Medical Genetics, University of Calgary, Calgary, Alberta, Canada.
Ko
Evelyn
E
Rademaker
Alfred
A
Chan
Peter
P
Robaire
Bernard
B
Martin
Renée H
RH
D000293:Adolescent; D000328:Adult; D000782:Aneuploidy; D002869:Chromosome Aberrations; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D014644:Genetic Variation; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D009630:Nondisjunction, Genetic / Q000502:physiology; D012016:Reference Values; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*; D024182:Uniparental Disomy / Q000473:pathology; D055815:Young Adult