title: Quantitative analysis of natural cyclodextrins by high-performance liquid chromatography with pulsed amperometric detection: application to cell permeation study.
abstract: Simple HPLC-PAD methods were developed for quantitation of cyclodextrins (CDs) in aqueous matrices from in vitro cell permeation studies. C-18 solid-phase extraction was used for sample pretreatment. Samples were analysed using acetonitrile-water mobile phase with post-column alkalization by 0.5M NaOH. Zorbax SB-Aq (for alpha-CD) and Zorbax SB-Phenyl (for beta-CD and gamma-CD) columns gave excellent peak shape and sufficient resolution of CD to glucose (2.7-3.2). The methods showed good concentration-response relationship (r > or = 0.999), precision (RSD% 0.7-5.1), repeatability (RSD% 3.4-13.7) and accuracy (87-107%). The limits of quantitation were 0.78, 0.46 and 0.52 microg/ml for alpha-CD, beta-CD and gamma-CD (RSD% of 10.6, 8.1 and 16.3, respectively).
authors.affiliation: Department of Pharmaceutical Chemistry, University of Kuopio, Finland. tarja.toropainen@uku.fi
authors.forename: Tarja
authors.initials: T
authors.lastname: Toropainen
authors.forename: Pekka
authors.initials: P
authors.lastname: Jarho
authors.forename: Marko
authors.initials: M
authors.lastname: Lehtonen
authors.forename: Pekka
authors.initials: P
authors.lastname: Keski-Rahkonen
authors.forename: Heli
authors.initials: H
authors.lastname: Raatikainen
authors.forename: Tomi
authors.initials: T
authors.lastname: Järvinen
mesh_terms: D002138:Calibration; D002463:Cell Membrane Permeability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003505:Cyclodextrins / Q000032:analysis*; D004563:Electrochemistry / Q000379:methods*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D003505:Cyclodextrins