title: Comparison of DNA pyrosequencing with alternative methods for identification of mycobacteria.
abstract: Identification of mycobacterial clinical isolates by pyrosequencing within the hypervariable A region of the 16S rRNA gene was compared to other identification methods. For >90% of isolates, these identifications correlated to the level of complex or species. For identification of many mycobacteria, pyrosequencing offers an inexpensive alternative to traditional sequencing.
authors.affiliation: Department of Pathology, Esoteric Testing, Tampa General Hospital, P.O. Box 1289, Tampa, FL 33601, USA. lheller@tgh.org
authors.forename: Loree C
authors.initials: LC
authors.lastname: Heller
authors.forename: Michael
authors.initials: M
authors.lastname: Jones
authors.forename: Ray H
authors.initials: RH
authors.lastname: Widen
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D006801:Humans; D009161:Mycobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009164:Mycobacterium Infections / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S