title: N-Acyl homoserine lactonase promotes prevention of Erwinia virulence with zwittermicin A-producing strain Bacillus cereus.
abstract: Zwittermicin A (ZwA), a hybrid polyketide- peptide antibiotic, and N-acyl -homoserine-lactonase (AHL-lactonase), a kind of quorum-quenching enzyme, have the potential to prevent the virulence of Erwinia carotovora, which is a major pathogen of soft rot. The purpose of this study was to combine the two agents in one host to improve their prevention efficacy. AHL-lactonase was over-expressed in the ZwA-producing strain Bacillus cereus and derivates were identified to create genetically modified (GM) strains. The comparative results showed that neither ZwA nor AHL-lactonase impacted the yield of the other; the increased yield of ZwA could promote the prevention efficacy of GM strains; Coexistence of ZwA and AHL-lactonase in the GM strains had better prevention efficacy than either has separately. It is a potential therapy to provide more effective prevention and withstand gradually increasing pesticide-resistance by combining the two antibacterial agents.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Life Science & Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Changming
authors.initials: C
authors.lastname: Zhao
authors.forename: Hui
authors.initials: H
authors.lastname: Zeng
authors.forename: Ziniu
authors.initials: Z
authors.lastname: Yu
authors.forename: Ming
authors.initials: M
authors.lastname: Sun
mesh_terms: D001409:Bacillus cereus / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics*; D002265:Carboxylic Ester Hydrolases / Q000235:genetics*; D004885:Erwinia / Q000472:pathogenicity*; D008666:Metalloendopeptidases / Q000235:genetics*; D010455:Peptides / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology*; D053038:Quorum Sensing; D014774:Virulence
chemical_list: D001426:Bacterial Proteins; D010455:Peptides; C088290:zwittermicin A; D002265:Carboxylic Ester Hydrolases; C432003:N-acyl homoserine lactonase; C406735:AiiA protein, Bacillus; D008666:Metalloendopeptidases