In vitro selection of RNA aptamers to a small molecule target.
This unit describes the selection of aptamers that bind to a small molecule target from a single-stranded RNA pool. Aptamers generated from this type of selection experiment can potentially function as receptors for small molecules for applications ranging from medical diagnostics to environmental monitoring. This unit describes two modes of selection, one by column filtration and one by batch selection.
Jhaveri
Sulay
S
Naval Research Laboratory, Washington, DC, USA.
Ellington
Andrew
A
D052157:Aptamers, Nucleotide / Q000302:isolation & purification*; D035843:Biomedical Research / Q000379:methods; D005374:Filtration; D008722:Methods; D052156:SELEX Aptamer Technique / Q000379:methods; D054852:Small Molecule Libraries
D052157:Aptamers, Nucleotide; D054852:Small Molecule Libraries