DNA mutation detection using denaturing high-performance liquid chromatography (DHPLC).
DHPLC is an efficient method for candidate gene scanning with a high level of automation. Single-base substitutions and insertions or deletions of up to 1.5 kb in PCR amplified DNA fragments can be detected. The method exploits the differential retention of homoduplex and heteroduplex DNA species under conditions of partial thermal denaturation. DHPLC provides a useful platform for high-throughput mutation detection and SNP discovery.
Yu
Bing
B
Department of Molecular and Clinical Genetics, Central Clinical School and SUPAMAC The University of Sydney, New South Wales, Australia.
Sawyer
Nicole A
NA
Chiu
Christine
C
Oefner
Peter J
PJ
Underhill
Peter A
PA
D019943:Amino Acid Substitution / Q000235:genetics; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods*; D006801:Humans; D009691:Nucleic Acid Denaturation
D004247:DNA