Influence of substance-P on cultured sebocytes.
Acne is a complex, chronic and common skin disorder of pilosebaceous units. Although it is known that exacerbation of acne results from emotional stress, the nature of the association between stress and acne remains unclear. This is due in part to the lack of substantial evidence regarding the participation of cutaneous neurogenic factors in the pathogenesis of acne. Culture of sebocytes provides a new insight into the participation of neuropeptides, notably substance P (SP), in the pathophysiology of acne. To examine the possible involvement of neurogenic factors in the pathogenesis of acne, we used immunohistochemistry and RT-PCR to compare the expression of interleukin-1 (IL-1), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), peroxisome proliferators activated receptors-gamma (PPAR-gamma) on the cultured sebocytes stimulated by SP. IL-1 is primarily proinflammatory cytokines to stimulate the expression of genes associated with inflammation. IL-6 is a pleiotropic cytokine with a wide range of biological activities and regulates inflammation. TNF-alpha is a pleiotropic pro-inflammatory cytokine that exerts multiple biologic effects. PPAR-gamma is a nuclear hormone receptor and plays a unique role in stimulating sebocyte lipogenesis. More numerous immunoreactivity to IL-1, IL-6, TNF-alpha and PPAR-gamma and increased RNA amplification for IL-1, IL-6, TNF-alpha and PPAR-gamma were observed after addition of SP compared with the control. This study reveals that SP is involved in the pathogenesis of acne.
Lee
Weon Ju
WJ
Department of Dermatology, Kyungpook National University School of Medicine, Jung-gu, Daegu, Republic of Korea. weonju@knu.ac.kr
Jung
Hong Dae
HD
Lee
Hyun Joo
HJ
Kim
Byung Soo
BS
Lee
Seok-Jong
SJ
Kim
Do Won
DW
D000152:Acne Vulgaris / Q000276:immunology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D004847:Epithelial Cells / Q000276:immunology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006801:Humans; D007249:Inflammation; D050155:Lipogenesis / Q000276:immunology; D015213:Neuroimmunomodulation / Q000276:immunology; D047495:PPAR gamma / Q000276:immunology* / Q000378:metabolism; D012627:Sebaceous Glands / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D012629:Sebum / Q000276:immunology / Q000378:metabolism; D013373:Substance P / Q000276:immunology / Q000378:metabolism* / Q000494:pharmacology
D016207:Cytokines; D047495:PPAR gamma; D013373:Substance P