title: Dietary prevention of malignant glioma aggressiveness, implications in oxidant stress and apoptosis.
abstract: Our study explored the influence of diet on gliomagenesis and associated systemic effects (SE) in rats. The experimental diet contained various ingredients supposed to interfere with carcinogenesis, mainly phytochemicals (PtcD for phytochemical diet) and its effects were compared to those of the same diet without the phytochemicals (BD for basal diet). Glioma was induced by ethylnitrosourea to pregnant females fed the diets from the start of gestation until the moment of sacrifice of the offpsrings. In male rats fed the PtcD or the BD the incidence of gliomas was markedly reduced compared to rats fed a standard diet (StD). In females this effect was weaker and was limited to the PtcD. A significant proportion of rats with brain tumors and fed the StD exhibited SE evidenced by weight loss, a shorter survival, reduction in liver weight and an increased proportion of liver mitochondria, effects that were not observed in their counterpart fed PtcD. Comparison of the expression of genes involved in the balance proliferation/apoptosis and in the response to oxidative stress in male brain tumors showed that the prevention of SE was associated with an increase in bcl-2 and catalase and a decrease in ki-67, sod-1 and sod-2 transcripts. These results show that the degree of agressiveness of gliomas can be modulated by dietary interventions and suggest that some phytochemicals with antioxidant properties could participate to the mechanism.
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Faculté de Médecine, Département de Recherche en Cancérologie, Université de Nantes, IFR26, F-44000, Nantes, France.
authors.forename: Daniel
authors.initials: D
authors.lastname: Pouliquen
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Laboratoire de Toxicologie, Faculté de Pharmacie, Université de Nantes, F-44000, Nantes, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Olivier
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Faculté de Médecine, Département de Recherche en Cancérologie, Université de Nantes, IFR26, F-44000, Nantes, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Hervouet
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Faculté de Médecine, Département de Recherche en Cancérologie, Université de Nantes, IFR26, F-44000, Nantes, France.
authors.forename: Francois
authors.initials: F
authors.lastname: Pedelaborde
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Faculté de Médecine, Département de Recherche en Cancérologie, Université de Nantes, IFR26, F-44000, Nantes, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Debien
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Faculté de Médecine, Département de Recherche en Cancérologie, Université de Nantes, IFR26, F-44000, Nantes, France.
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Le Cabellec
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Faculté de Médecine, Département de Recherche en Cancérologie, Université de Nantes, IFR26, F-44000, Nantes, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Gratas
authors.affiliation: Pathology group, International Agency for Research on Cancer (IARC), Lyon, France.
authors.forename: Taku
authors.initials: T
authors.lastname: Homma
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Faculté de Médecine, Département de Recherche en Cancérologie, Université de Nantes, IFR26, F-44000, Nantes, France.
authors.forename: Khaled
authors.initials: K
authors.lastname: Meflah
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Faculté de Médecine, Département de Recherche en Cancérologie, Université de Nantes, IFR26, F-44000, Nantes, France.
authors.forename: Francois M
authors.initials: FM
authors.lastname: Vallette
authors.affiliation: Département de Recherche en Cancérologie, Institut de Biologie, Inserm, U601, Equipe Apoptose et progression tumorale, F-44000, Nantes, France.|Faculté de Médecine, Département de Recherche en Cancérologie, Université de Nantes, IFR26, F-44000, Nantes, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Menanteau
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D014408:Biomarkers, Tumor / Q000032:analysis; D001932:Brain Neoplasms / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D049109:Cell Proliferation / Q000187:drug effects; D005038:Ethylnitrosourea; D005260:Female; D005638:Fruit; D005910:Glioma / Q000139:chemically induced / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control*; D007150:Immunohistochemistry; D008297:Male; D008930:Mitochondria, Liver / Q000378:metabolism; D018384:Oxidative Stress; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012334:RNA, Neoplasm / Q000032:analysis; D051381:Rats; D014675:Vegetables
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D014408:Biomarkers, Tumor; D012334:RNA, Neoplasm; D005038:Ethylnitrosourea