title: [SNP discovery by F-CSGE in the coding region of mitochondrial DNA in wild house mice from Shanghai suburb].
abstract: This study was performed to discover SNPs for genetic polymorphism analysis of mitochondrial DNA from wild house mice. Universal primer florescent PCR, fluorescence-based conformation sensitive gel electrophoresis (F-CSGE) and DNA sequencing were conducted to analyze the coding region of mitochondrial DNA. Different types of unknown mutations were recorded by variable F-CSGE patterns without false positive. Twenty-four SNPs, sixteen of which were first discovered in the coding region of mitochondrial DNA, were found in 64 wild house mice from 4 districts in Shanghai. Therefore, F-CSGE was proved to be powerful technique for SNP discovery in the coding region mitochondrial DNA. The novel SNPs can be used as molecular markers to analyze population structure and genetic polymorphisms of the wild house mice in Shanghai.
authors.affiliation: Institute of Biology Science & Technology, Donghua University, Shanghai 201620, China. pawffl@163.com
authors.forename: Zhong-Peng
authors.initials: ZP
authors.lastname: Fan
authors.forename: Wang-Sheng
authors.initials: WS
authors.lastname: Zhu
authors.forename: Chao
authors.initials: C
authors.lastname: Zhang
authors.forename: Yu-Xun
authors.initials: YX
authors.lastname: Zhou
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.forename: Yin-Ming
authors.initials: YM
authors.lastname: Liang
authors.forename: Zheng-Hong
authors.initials: ZH
authors.lastname: Xing
authors.forename: Guo-Qiang
authors.initials: GQ
authors.lastname: Chen
authors.forename: Xiong
authors.initials: X
authors.lastname: Bai
authors.forename: Jun-Hua
authors.initials: JH
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D004586:Electrophoresis / Q000379:methods*; D051379:Mice; D008957:Models, Genetic; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial