Hydrophobic-interaction chromatography.
This unit discusses the important parameters in designing and optimizing a separation by hydrophobic interaction chromatography (HIC), including preparing the sample and choosing a matrix, column, and buffer. Protocols are provided for packing and testing a column; determining binding and elution conditions; eluting the sample; and cleaning, regenerating, and storing HIC columns.
Kennedy
R M
RM
Pharmacia Biotech, Piscataway, New Jersey, USA.
D002845:Chromatography / Q000295:instrumentation / Q000379:methods*; D057927:Hydrophobic and Hydrophilic Interactions; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
D011506:Proteins