Detection assays for HIV proteins.
This updated unit describes four assays for detection of HIV proteins including: (1) a quantitative ELISA, (2) a quantitative immunoblotting assay, (3) a qualitative immunofluorescence assay, and (4) a functional assay to measure virus-associated reverse transcriptase activity as an indicator of viral production.
Ostrowski
Mario A
MA
University of Toronto, Toronto, Canada.
Chun
Tae-Wook
TW
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
Cheseboro
Bruce
B
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
Stanley
Sharilyn K
SK
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland.
Tremblay
Michel
M
University of Laval, Quebec, Canada.
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005434:Flow Cytometry / Q000379:methods*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D015488:HIV Antigens / Q000032:analysis* / Q000276:immunology; D054303:HIV Reverse Transcriptase / Q000032:analysis*; D006801:Humans; D015151:Immunoblotting / Q000379:methods*
D015488:HIV Antigens; D054303:HIV Reverse Transcriptase