The role of nuclear factor kappa B in lens epithelial cell proliferation using a capsular bag model.
OBJECTIVE
This study was performed to elucidate the role of nuclear factor kappaB (NF-kappaB) in lens epithelial cell proliferation in the capsular bag model for this study.


METHODS
Capsular bags were prepared from porcine eyes and cultured in a 5% CO(2) atmosphere at 37 degrees C. NF-kappaB translocation was confirmed by immunohistochemistry and Western blot analysis. The effects of sulfasalazine and SN50 peptide, inhibitors of NF-kappaB activation, were observed by light microscopy and scanning electron microscopy.


RESULTS
NF-kappaB was found in the cytoplasm of nonproliferated lens epithelial cells. However, NF-kappaB moved to the nucleus in proliferation cells, proliferating-cell-nuclear-antigen-positive cells. This translocation was inhibited by treatment with sulfasalazine or NF-kappaB SN50 peptide. These inhibitors also blocked lens epithelial cell migration from the equatorial to the posterior capsule.


CONCLUSIONS
NF-kappaB controls proliferation and regulates the growth of lens epithelial cells. In this study, sulfasalazine and NF-kappaB SN50 peptide inhibited cell proliferation in the capsular bag model. These results suggest that the regulation of NF-kappaB in lens epithelial cells may modulate posterior capsule opacification.
Lee
Seok-Jong
SJ
Department of Ophthalmology and Visual Science, College of Medicine, Catholic University of Korea, Seoul, Korea.
Bae
Sangwoo
S
Seomun
Young
Y
Son
Min-Jung
MJ
Joo
Choun-Ki
CK
D000818:Animals; D015153:Blotting, Western; D002386:Cataract / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D007903:Lens Capsule, Crystalline / Q000166:cytology* / Q000378:metabolism; D007908:Lens, Crystalline / Q000166:cytology* / Q000378:metabolism; D008855:Microscopy, Electron, Scanning; D008954:Models, Biological; D016328:NF-kappa B / Q000037:antagonists & inhibitors / Q000502:physiology*; D010455:Peptides / Q000494:pharmacology; D012460:Sulfasalazine / Q000494:pharmacology; D013552:Swine
D016328:NF-kappa B; D010455:Peptides; C118258:SN50 peptide; D012460:Sulfasalazine