Identification of a porcine calicivirus related genetically to human sapoviruses.
Whether animals may act as reservoirs for human caliciviruses is unclear. By sequence analysis of a short fragment of the RNA-dependent RNA polymerase (RdRp) region, porcine sapovirus (SaV) strains that genetically resemble human SaVs have been detected in piglets, but more-informative sequences (capsid gene) were not available for a precise characterization. In this study, the 3' terminus (the 3' end of open reading frame 1 [ORF1], including the polymerase complex and the complete capsid; ORF2; and the 3' untranslated region) of one such human SaV-like strain, 43/06-18p3/2006/It, was determined, revealing that these viruses are more related genetically to human (47.4 to 54.9% amino acid identity) than to animal (35.2 to 44.7% amino acid identity) SaVs in the capsid gene. In addition, the recombination-prone RdRp-capsid junction region was highly conserved with those of human SaVs of genogroup GI. The presence of porcine viruses similar to human SaVs is a significant finding because of the potential for zoonotic infections or generation of porcine/human recombinants.
Martella
V
V
Dipartimento di Sanità Pubblica e Zootecnia, Facoltà di Medicina Veterinaria di Bari, S.p. per Casamassima Km 3, 70010 Valenzano, Bari, Italy. v.martella@veterinaria.uniba.it
Lorusso
E
E
Banyai
K
K
Decaro
N
N
Corrente
M
M
Elia
G
G
Cavalli
A
A
Radogna
A
A
Costantini
V
V
Saif
L J
LJ
Lavazza
A
A
Di Trani
L
L
Buonavoglia
C
C
D000818:Animals; D001483:Base Sequence; D002139:Caliciviridae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D017250:Caliciviridae Infections / Q000662:veterinary / Q000821:virology*; D002213:Capsid / Q000737:chemistry; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000662:veterinary / Q000821:virology; D006801:Humans; D007231:Infant, Newborn; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000302:isolation & purification; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry / Q000235:genetics; D029341:Sapovirus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D013552:Swine / Q000821:virology*; D013553:Swine Diseases / Q000821:virology*
D012367:RNA, Viral; D012324:RNA-Dependent RNA Polymerase