title: Culture-Negative intracerebral abscesses in children and adolescents from Streptococcus anginosus group infection: a case series.
abstract: We report the use of 16S ribosomal RNA gene amplification and sequencing to diagnose culture-negative intracerebral abscesses in younger patients. These 3 cases demonstrate the optimal application of gene sequencing from direct specimens for patients with negative culture results compromised by antibacterial therapy but histories highly suggestive of acute bacterial infection.
authors.affiliation: ARUP Laboratories, University of Utah School of Medicine, Salt Lake City, UT 84108, USA. cathy.petti@aruplab.com
authors.forename: Cathy A
authors.initials: CA
authors.lastname: Petti
authors.forename: Keith E
authors.initials: KE
authors.lastname: Simmon
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Bender
authors.forename: Anne
authors.initials: A
authors.lastname: Blaschke
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Webster
authors.forename: Mark F
authors.initials: MF
authors.lastname: Conneely
authors.forename: Paul C
authors.initials: PC
authors.lastname: Schreckenberger
authors.forename: Thomas C
authors.initials: TC
authors.lastname: Origitano
authors.forename: Malliswari
authors.initials: M
authors.lastname: Challapalli
mesh_terms: D000293:Adolescent; D000328:Adult; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001921:Brain / Q000000981:diagnostic imaging; D001922:Brain Abscess / Q000382:microbiology*; D002648:Child; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008969:Molecular Sequence Data; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011859:Radiography; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000382:microbiology*; D034366:Streptococcus anginosus / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S