Understanding water equilibration fundamentals as a step for rational protein crystallization.
BACKGROUND
Vapor diffusion is the most widely used technique for protein crystallization and the rate of water evaporation plays a key role on the quality of the crystals. Attempts have been made in the past to solve the mass transfer problem governing the evaporation process, either analytically or by employing numerical methods. Despite these efforts, the methods used for protein crystallization remain based on trial and error techniques rather than on fundamental principles.


RESULTS
Here we present a new theoretical model which describes the hanging drop method as a function of the different variables that are known to influence the evaporation process. The model is extensively tested against experimental data published by other authors and considering different crystallizing conditions. Aspects responsible for the discrepancies between the existing theories and the measured evaporation kinetics are especially discussed; they include the characterization of vapor-liquid equilibrium, the role of mass transfer within the evaporating droplet, and the influence of the droplet-reservoir distance.


CONCLUSIONS
The validation tests show that the proposed model can be used to predict the water evaporation rates under a wide range of experimental conditions used in the hanging drop vapor-diffusion method, with no parameter fitting or computational requirements. This model combined with protein solubility data is expected to become a useful tool for a priori screening of crystallization conditions.
Martins
Pedro M
PM
Grupo de Estrutura Molecular, Instituto de Biologia Molecular e Celular (IBMC), Universidade do Porto, Porto, Portugal.
Rocha
Fernando
F
Damas
Ana M
AM
D003460:Crystallization / Q000379:methods*; D008956:Models, Chemical; D011312:Pressure; D011506:Proteins / Q000737:chemistry*; D015203:Reproducibility of Results; D014835:Volatilization; D014867:Water / Q000737:chemistry*
D011506:Proteins; D014867:Water