Production of polyclonal antisera.
Antibodies are serum immunoglobulins with binding specificity for particular antigens, and polyclonal antibodies are particularly valuable for immunoprecipitation and immunoblotting. In this unit, the production of polyclonal antisera specific for protein antigens in rabbits, rats, mice, and hamsters is described. A support protocol is included for preparing serum from blood.
Cooper
H M
HM
Ludwig Institute for Cancer Research, Melbourne, Australia.
Paterson
Y
Y
D000818:Animals; D000941:Antigens / Q000235:genetics / Q000276:immunology*; D002999:Clone Cells / Q000276:immunology / Q000378:metabolism; D006224:Cricetinae; D007106:Immune Sera / Q000096:biosynthesis* / Q000097:blood / Q000235:genetics; D007136:Immunoglobulins / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D011817:Rabbits; D051381:Rats
D000941:Antigens; D007106:Immune Sera; D007136:Immunoglobulins