title: Influence of pH upon surface-enhanced enzyme-catalyzed luminol chemiluminescence at vicinity of nanoscale-corrugated gold and silver films.
abstract: Au and Ag biochips were fabricated to investigate the influence of pH upon the chemiluminescence (CL) of luminol at vicinity of surface-adsorbed peroxidase. A nanoscaled-corrugation of the metal induces an enhancement of the luminol CL which is maximal in the pH range favoring peroxidase catalysis and greater for gold than for silver. This is the proof that, in the CL process, the reactions involving peroxidase are surface-enhanced near corrugated surfaces.
authors.affiliation: MATEIS, INSA-Lyon, Université de Lyon, Villeurbanne, France.
authors.forename: Meigui
authors.initials: M
authors.lastname: Ou
authors.forename: Guowei
authors.initials: G
authors.lastname: Lu
authors.forename: Hong
authors.initials: H
authors.lastname: Shen
authors.forename: Armel
authors.initials: A
authors.lastname: Descamps
authors.forename: Christophe André
authors.initials: CA
authors.lastname: Marquette
authors.forename: Loïc Jacques
authors.initials: LJ
authors.lastname: Blum
authors.forename: Stéphane
authors.initials: S
authors.lastname: Roux
authors.forename: Olivier
authors.initials: O
authors.lastname: Tillement
authors.forename: Bolin
authors.initials: B
authors.lastname: Cheng
authors.forename: Pascal
authors.initials: P
authors.lastname: Perriat
mesh_terms: D000327:Adsorption; D055162:Biocatalysis; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D049449:Luminescence; D008165:Luminol / Q000737:chemistry* / Q000378:metabolism*; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology; D010316:Particle Size; D009195:Peroxidase / Q000378:metabolism*; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008567:Membranes, Artificial; D012834:Silver; D008165:Luminol; D006046:Gold; D006861:Hydrogen Peroxide; D009195:Peroxidase