title: Assessment of day-3 morphology and euploidy for individual chromosomes in embryos that develop to the blastocyst stage.
abstract: OBJECTIVE
  To examine the relationship between day-3 morphology and euploidy for individual chromosomes in embryos that develop to the blastocyst stage by day 5.
  
  
  METHODS
  Retrospective cohort study.
  
  
  METHODS
  Boston IVF, a large university-affiliated reproductive medicine practice.
  
  
  METHODS
  Ninety-nine patients undergoing their first preimplantation genetic screening (PGS) cycle between January 1 and December 31, 2006.
  
  
  METHODS
  In vitro fertilization (IVF) and preimplantation genetic screening (PGS).
  
  
  METHODS
  Prevalence of euploidy for chromosomes X, Y, 8, 13, 14, 15, 16, 17, 18, 20, 21, and 22 in day-3 high implantation potential (HIP) versus non-HIP embryos that grew to day-5 blastocysts.
  
  
  RESULTS
  Seven hundred three embryos from 99 cycles in 99 patients underwent PGS. Three hundred sixty-four (52%) embryos from 88 cycles in 88 patients developed to the blastocyst stage by day 5. High implantation potential embryos were more likely to be euploid for chromosomes X/Y, 8, 15, 16, 18, and 22 compared with non-HIP embryos, with similar trends for chromosomes 14 and 17. There were no statistically significant differences between HIP and non-HIP embryos in euploidy prevalence for chromosomes 13, 20, and 21.
  
  
  CONCLUSIONS
  Our data suggest that PGS may detect potentially viable but detrimental chromosomal abnormalities that are not detected by embryo morphology alone.
authors.affiliation: Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Eaton
authors.forename: Michele R
authors.initials: MR
authors.lastname: Hacker
authors.forename: Doria
authors.initials: D
authors.lastname: Harris
authors.forename: Kim L
authors.initials: KL
authors.lastname: Thornton
authors.forename: Alan S
authors.initials: AS
authors.lastname: Penzias
mesh_terms: D001755:Blastocyst / Q000166:cytology / Q000502:physiology*; D002869:Chromosome Aberrations / Q000706:statistics & numerical data; D002877:Chromosomes, Human / Q000502:physiology*; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D010064:Embryo Implantation / Q000502:physiology*; D004622:Embryo, Mammalian / Q000502:physiology*; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D005820:Genetic Testing / Q000379:methods; D006801:Humans; D011003:Ploidies; D011247:Pregnancy