title: TILLMore, a resource for the discovery of chemically induced mutants in barley.
abstract: A sodium azide-mutagenized population of barley (cv. 'Morex') was developed and utilized to identify mutants at target genes using the 'targeting induced local lesions in genomes' (TILLING) procedure. Screening for mutations at four agronomically important genes (HvCO1, Rpg1, eIF4E and NR) identified a total of 22 new mutant alleles, equivalent to the extrapolated rate of one mutation every 374 kb. All mutations except one were G/C to A/T transitions and several (approximately 68%) implied a change in protein amino acid sequence and therefore a possible effect on phenotype. The high rate of mutation detected through TILLING is in keeping with the high frequency (32.7%) of variant phenotypes observed amongst the M(3) families. Our results indicate the feasibility of using this resource for both reverse and forward genetics approaches to investigate gene function in barley and related crops.
authors.affiliation: Department of Agroenvironmental Sciences and Technology, University of Bologna, Viale Fanin 44, 40127 Bologna, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Talamè
authors.forename: Riccardo
authors.initials: R
authors.lastname: Bovina
authors.forename: Maria Corinna
authors.initials: MC
authors.lastname: Sanguineti
authors.forename: Roberto
authors.initials: R
authors.lastname: Tuberosa
authors.forename: Udda
authors.initials: U
authors.lastname: Lundqvist
authors.forename: Silvio
authors.initials: S
authors.lastname: Salvi
mesh_terms: D018744:DNA, Plant / Q000032:analysis / Q000235:genetics; D018390:Gene Targeting; D017343:Genes, Plant; D001467:Hordeum / Q000187:drug effects* / Q000235:genetics*; D016296:Mutagenesis / Q000187:drug effects*; D009154:Mutation / Q000235:genetics*; D010641:Phenotype; D019810:Sodium Azide / Q000494:pharmacology*
chemical_list: D018744:DNA, Plant; D019810:Sodium Azide