A sensitive HPLC-MS-MS method for the determination of raltegravir in human plasma.
This work describes an assay system that has been developed to quantify raltegravir concentrations in human plasma using a liquid-liquid extraction technique paired with HPLC separation and MS-MS detection. The dynamic range of this assay extends from 1 to 3000 ng/mL, with a coefficient of determination (r(2), mean+/-SD) of 0.9992+/-0.0002. The mean precision values for calibration standards ranged from 0.6% to 3.0%, while accuracy values were 96.5-104.3%. This procedure is an accurate, precise, and sensitive method for raltegravir quantitation and was successfully validated using external proficiency testing.
Long
Mary C
MC
Division of Clinical Pharmacology, University of Alabama at Birmingham School of Medicine, Birmingham, AL 35294-0019, United States.
Bennetto-Hood
Chantelle
C
Acosta
Edward P
EP
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019428:HIV Integrase Inhibitors / Q000097:blood*; D006801:Humans; D011760:Pyrrolidinones / Q000097:blood*; D011786:Quality Control; D000068898:Raltegravir Potassium; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D019428:HIV Integrase Inhibitors; D011760:Pyrrolidinones; D000068898:Raltegravir Potassium