Primate lentivirus capsid sensitivity to TRIM5 proteins.
Mammalian cells express several factors that inhibit lentiviral infection and that have been under strong selective pressure. One of these factors, TRIM5, targets the capsid protein of incoming retrovirus particles and inhibits subsequent steps of the replication cycle. By substituting human immunodeficiency virus type 1 capsid, we were able to show that a set of divergent primate lentivirus capsids was generally not susceptible to restriction by TRIM5 proteins from higher primates. TRIM5alpha proteins from other primates exhibited distinct restriction specificities for primate lentivirus capsids. Finally, we identified novel primate lentiviral capsids that are targeted by TRIMCyp proteins.
Kratovac
Zerina
Z
Aaron Diamond AIDS Research Center and the Rockefeller University, New York, NY 10016, USA.
Virgen
Cesar A
CA
Bibollet-Ruche
Frederick
F
Hahn
Beatrice H
BH
Bieniasz
Paul D
PD
Hatziioannou
Theodora
T
D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D016466:CHO Cells; D036022:Capsid Proteins / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D005434:Flow Cytometry; D015497:HIV-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D017958:Lentiviruses, Primate / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D011323:Primates / Q000378:metabolism*; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D016415:Sequence Alignment; D013045:Species Specificity; D044767:Ubiquitin-Protein Ligases
D036022:Capsid Proteins; D011506:Proteins; C482587:TRIM5(alpha) protein, rhesus monkey; D044767:Ubiquitin-Protein Ligases