title: Fluorescent in situ hybridization as a screening test for HER2 amplification in G2 and G3 breast cancers of lobular and ductal histotype and metastases.
abstract: The aim of the present study was to evaluate the effectiveness of fluorescence in situ hybridisation (FISH), as a screening test, in moderately- (G2) or poorly- (G3) differentiated breast cancers of the ductal (IDC) and lobular (ILC) histotypes and distant metastases. HER2 FISH was performed on 486 G2 and 477 G3 both of IDC and ILC histotypes and in 241 metastases. A significant difference in the HER2 amplification was observed between G2 (14.8%) and G3 (31.9%), with no difference according to the histotype. However, the rate of amplification increased to 36% in the G2/hormone receptor-negative cases as compared to 10.6% in the G2/receptor-positive cases (p<0.0001). HER2 was amplified in 17% of metastases with some differences depending on the location. These data suggest that the HER2 FISH analysis may be an effective screening test in breast cancer metastases and G3 tumors, irrespective of the hormone receptor status or presence of lymphovascular invasion.
authors.affiliation: Department of Biomedical Sciences and Human Oncology, University of Turin, 10126 Turin, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Castellano
authors.forename: Anna
authors.initials: A
authors.lastname: Sapino
authors.forename: Riccardo
authors.initials: R
authors.lastname: Arisio
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Viale
authors.forename: Gianni
authors.initials: G
authors.lastname: Bussolati
authors.forename: Roberto
authors.initials: R
authors.lastname: Bandelloni
authors.forename: Gaetano
authors.initials: G
authors.lastname: Barresi
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bersiga
authors.forename: Cesare
authors.initials: C
authors.lastname: Bordi
authors.forename: Gerardo
authors.initials: G
authors.lastname: Botti
authors.forename: Fabia
authors.initials: F
authors.lastname: Cosimi
authors.forename: Emanuele
authors.initials: E
authors.lastname: D'Amore
authors.forename: Claudio
authors.initials: C
authors.lastname: Doglioni
authors.forename: Antonio
authors.initials: A
authors.lastname: Marchetti
authors.forename: Oscar
authors.initials: O
authors.lastname: Nappi
authors.forename: Francesco
authors.initials: F
authors.lastname: Romeo
authors.forename: Massimo
authors.initials: M
authors.lastname: Roncalli
authors.forename: Rosa
authors.initials: R
authors.lastname: Russo
authors.forename: Alfredo
authors.initials: A
authors.lastname: Santinelli
authors.forename: Luigi Giusto
authors.initials: LG
authors.lastname: Spagnoli
authors.forename: Francesco
authors.initials: F
authors.lastname: Tanda
authors.forename: Paolo
authors.initials: P
authors.lastname: Tricomi
authors.forename: Gianpaolo
authors.initials: G
authors.lastname: Trentini
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Zanconati
authors.forename: Monica
authors.initials: M
authors.lastname: Iurlaro
mesh_terms: D005260:Female; D018734:Genes, erbB-2 / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008403:Mass Screening / Q000379:methods; D008495:Medical Oncology / Q000379:methods; D015233:Models, Statistical; D015999:Multivariate Analysis; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging / Q000379:methods; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis*; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D018719:Erb-b2 Receptor Tyrosine Kinases; C508053:ERBB2 protein, human