Transplantation of adipose derived stromal cells is associated with functional improvement in a rat model of chronic myocardial infarction.
OBJECTIVE
To determine the effect of transplantation of undifferentiated and cardiac pre-differentiated adipose stem cells compared with bone marrow mononuclear cells (BM-MNC) in a chronic model of myocardial infarction.


METHODS
Ninety-five Sprague-Dawley rats underwent left coronary artery ligation and after 1 month received by direct intramyocardial injection either adipose derived stem cells (ADSC), cardiomyogenic cells (AD-CMG) or BM-MNC from enhanced-Green Fluorescent Protein (eGFP) mice. The control group was treated with culture medium. Heart function was assessed by echocardiography and 18F-FDG microPET. Cell engraftment, differentiation, angiogenesis and fibrosis in the scar tissue were also evaluated by (immuno)histochemistry and immunofluorescence.


RESULTS
One month after cell transplantation, ADSC induced a significant improvement in heart function (LVEF 46.3+/-9.6% versus 27.7+/-8% pre-transplant) and tissue viability (64.78+/-7.2% versus 55.89+/-6.3% pre-transplant). An increase in the degree of angiogenesis and a decrease in fibrosis were also detected. Although transplantation of AD-CMG or BM-MNC also had a positive, albeit smaller, effect on angiogenesis and fibrosis in the infarcted hearts, this benefit did not translate into a significant improvement in heart function or tissue viability.


CONCLUSIONS
These results indicate that transplantation of adipose derived cells in chronic infarct provides a superior benefit to cardiac pre-differentiated ADSC and BM-MNC.
Mazo
Manuel
M
Hematology and Cell Therapy and Foundation for Applied Medical Research, Division of Cancer, University of Navarra, Pamplona, Spain.
Planat-Bénard
Valérie
V
Abizanda
Gloria
G
Pelacho
Beatriz
B
Léobon
Bertrand
B
Gavira
Juan José
JJ
Peñuelas
Iván
I
Cemborain
Arantxa
A
Pénicaud
Luc
L
Laharrague
Patrick
P
Joffre
Carine
C
Boisson
Marie
M
Ecay
Margarita
M
Collantes
Maria
M
Barba
Joaquin
J
Casteilla
Louis
L
Prósper
Felipe
F
D000273:Adipose Tissue / Q000166:cytology; D000818:Animals; D016026:Bone Marrow Transplantation; D002908:Chronic Disease; D005260:Female; D007963:Leukocytes, Mononuclear / Q000637:transplantation; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D051379:Mice; D008810:Mice, Inbred C57BL; D009203:Myocardial Infarction / Q000628:therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D012038:Regeneration; D017154:Stromal Cells / Q000637:transplantation*