p14ARF is capable of promoting HIV-1 tat degradation.
The p14(ARF) tumor suppressor functions as 'oncogenic checkpoint' that prevents unrestricted cellular proliferation in response to oncogenic signaling. Albeit, the major pathway through which ARF operates is the ARF-Mdm2-p53 axis, ARF directly binds to and inactivates transcription function of a number of DNA-bound activators. In the present study we show that p14(ARF) inhibits transcription activation of HIV-1 LTR promoter activity by Tat protein. Tat protein is a RNA-bound transcriptional activator whose function is strictly required for HIV-1 replication. We determined that p14(ARF) inhibits Tat transactivation of HIV-1 LTR by promoting Tat degradation via an ubiquitin-independent pathway.
Gargano
Barbara
B
Department of Structural and Functional Biology, University of Naples Federico II, Naples, Italy.
Fiorillo
Marianna
M
Amente
Stefano
S
Majello
Barbara
B
Lania
Luigi
L
D002850:Chromatography, Gel; D015967:Gene Expression Regulation, Viral / Q000502:physiology*; D015696:Gene Products, tat / Q000378:metabolism*; D016325:HIV Long Terminal Repeat / Q000235:genetics* / Q000502:physiology; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation; D025803:Tumor Suppressor Protein p14ARF / Q000378:metabolism*
D015696:Gene Products, tat; D025803:Tumor Suppressor Protein p14ARF