Receptor binding techniques.
This overview first discusses issues relating to the selection of radioligand for receptor binding assays, including the isotopic label and considerations pertaining to the pharmacological and chemical profile of the ligand. This is followed by a section on characterization of ligand-binding assays, starting with tissue preparation methods, followed by detection of specific binding, determination of incubation and washing conditions and a discussion of saturation and competition assay formats. Quantification of the assay results can be accomplished by autoradiography or film densitometry. Finally, methods and considerations for analysis of the resulting data are presented.
Frey
K A
KA
University of Michigan, Ann Arbor, Michigan, USA.
Albin
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RL
D000818:Animals; D001345:Autoradiography / Q000379:methods / Q000639:trends; D001667:Binding, Competitive; D006801:Humans; D007202:Indicators and Reagents / Q000378:metabolism; D008024:Ligands; D011485:Protein Binding; D011868:Radioisotopes / Q000378:metabolism; D011869:Radioligand Assay / Q000379:methods* / Q000639:trends*; D011956:Receptors, Cell Surface / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
D007202:Indicators and Reagents; D008024:Ligands; D011868:Radioisotopes; D011956:Receptors, Cell Surface