title: swissPIT: a novel approach for pipelined analysis of mass spectrometry data.
abstract: The identification and characterization of peptides from tandem mass spectrometry (MS/MS) data represents a critical aspect of proteomics. Today, tandem MS analysis is often performed by only using a single identification program achieving identification rates between 10-50% (Elias and Gygi, 2007). Beside the development of new analysis tools, recent publications describe also the pipelining of different search programs to increase the identification rate (Hartler et al., 2007; Keller et al., 2005). The Swiss Protein Identification Toolbox (swissPIT) follows this approach, but goes a step further by providing the user an expandable multi-tool platform capable of executing workflows to analyze tandem MS-based data. One of the major problems in proteomics is the absent of standardized workflows to analyze the produced data. This includes the pre-processing part as well as the final identification of peptides and proteins. The main idea of swissPIT is not only the usage of different identification tool in parallel, but also the meaningful concatenation of different identification strategies at the same time. The swissPIT is open source software but we also provide a user-friendly web platform, which demonstrates the capabilities of our software and which is available at http://swisspit.cscs.ch upon request for account.
authors.affiliation: Swiss Institute of Bioinformatics, Proteome Informatics Group, Geneva, Geneva Bioinformatics (GeneBio) S.A., Geneva, Switzerland. andreas.quandt@isb-sib.ch
authors.forename: Andreas
authors.initials: A
authors.lastname: Quandt
authors.forename: Patricia
authors.initials: P
authors.lastname: Hernandez
authors.forename: Alexandre
authors.initials: A
authors.lastname: Masselot
authors.forename: Céline
authors.initials: C
authors.lastname: Hernandez
authors.forename: Sergio
authors.initials: S
authors.lastname: Maffioletti
authors.forename: Cesare
authors.initials: C
authors.lastname: Pautasso
authors.forename: Ron D
authors.initials: RD
authors.lastname: Appel
authors.forename: Frederique
authors.initials: F
authors.lastname: Lisacek
mesh_terms: D000465:Algorithms; D000595:Amino Acid Sequence; D013058:Mass Spectrometry / Q000379:methods*; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D011506:Proteins / Q000737:chemistry*; D020539:Sequence Analysis, Protein / Q000379:methods*; D012984:Software
chemical_list: D011506:Proteins