title: Chromatofocusing.
abstract: This unit discusses choice of chromatofocusing medium and buffers, determination of the quantity of medium needed, and preparation of CF buffers. A protocol is provided for a typical chromatofocusing experiment, and support protocols describe regeneration of the chromatofocusing column and several methods for separation of the polybuffer from the purified protein.
authors.affiliation: Pharmacia Biotech, Piscataway, New Jersey, USA.
authors.forename: A
authors.initials: A
authors.lastname: Williams
mesh_terms: D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011506:Proteins