[The mechanism of HBV infection of human trophoblast cell].
OBJECTIVE
To observe the changes of human trophoblast cells after infected with hepatitis B virus.


METHODS
HBV positive serum was used to infect human trophoblast cells in vitro. HBsAg in cell culture medium were detected by ELISA method and HBV DNA in cell culture medium and cells were detected by PCR method. HBV fluorescence polymerase chain reaction diagnose kit were used to detect the HBV DNA concentration. Ultra structure of trophoblast cells were observed with transmission electron microscopy (TEM).


RESULTS
HBsAg could be detected in infection group by ELISA. Infection group cell culture medium and infection group cells were HBV DNA positive. HBV DNA concentrations in HBV infection cell culture medium in 0, 12, 36, 60, 84 h after extensively PBS washed were < 10(3), 3 x 10(4), 6 x 10(5), 5 x 10(5), 3 x 10(5) copies/mL. HBV infected trophoblast cells were found many forms of endosomes, some of which contents virus like particle.


CONCLUSIONS
HBV might take advantage of clathrin-mediated endocytosis to enter trophoblast cell, which might lead to cell infection or across the cell bar by transcytosis.
Wang
An-hui
AH
Department of Epidemiology, School of Preventive Medicine, Fourth Military Medical University, Xi'an 710033, China. wanganhui@hotmail.com
Wang
Ai-qin
AQ
Xu
De-zhong
DZ
Men
Ke
K
Yan
Yong-ping
YP
Zhang
Jing-xia
JX
Liu
Yuan
Y
Huang
Xiao-feng
XF
Wang
Chun-mei
CM
D000818:Animals; D017077:Culture Media, Conditioned / Q000378:metabolism; D004279:DNA, Viral / Q000032:analysis; D011992:Endosomes / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D006514:Hepatitis B Surface Antigens / Q000032:analysis; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D016133:Polymerase Chain Reaction; D013997:Time Factors; D014327:Trophoblasts / Q000648:ultrastructure / Q000821:virology*
D017077:Culture Media, Conditioned; D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens