Gene transduction by sonoporation.
Gene transduction technologies are essential tools for understanding of gene functions or gene cascades underlying embryogenesis. In this review, we introduce a gene transduction method using microbubble and ultrasound (hereafter referred to as sonoporation). Sonoporation is carried out with relatively simple procedures and easily transduces genes into mesenchymal cells without significant damage to target tissues. Therefore, sonoporation is effective for gene transduction to study the molecular mechanisms of morphogenesis.
Ohta
Sho
S
Department of Neurobiology and Anatomy, School of Medicine, University of Utah, Salt Lake City, Utah, USA.
Suzuki
Kentaro
K
Ogino
Yukiko
Y
Miyagawa
Shinichi
S
Murashima
Aki
A
Matsumaru
Daisuke
D
Yamada
Gen
G
D000818:Animals; D002642:Chick Embryo; D018274:Electroporation / Q000379:methods; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D018878:Limb Buds / Q000196:embryology; D008648:Mesoderm / Q000166:cytology; D045423:Microbubbles; D008957:Models, Genetic; D013010:Sonication; D014161:Transduction, Genetic
D049452:Green Fluorescent Proteins