[Primary research on detecting bacteria drug resistance by matrix-assisted laxer desorption ionization time-of-flight mass spectrometry].
OBJECTIVE
To study the feasibility of bacteria drug resistance determinated by matrix-assisted laxer desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS).


METHODS
11 strains of 8 genera in drug resistant-bacteria separated from clinical specimens and the corresponding standard strains were detected by MALDI-TOF-MS, and the differences of the mass spectrums were analyzed. At the same time, 30 strains of Staphylococcus aureus separated from clinical specimens were detected by MALDI-TOF-MS and their antimicrobial susceptibility were tested. All bacteria were cultured under the same conditions.


RESULTS
When drug-resistant bacteria determinated by MALDI-TOF-MS, it was efficient for Staphylococcus, especially the methicillin-resistant Staphylococcus aureus, but it was not efficient for other genera.


CONCLUSIONS
MALDI-TOF-MS can be used to detect drug resistance of some bacteria.
Shi
Guiying
G
Institute for Environmental Health and Related Product Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China.
Sun
Zongke
Z
Chen
Xiping
X
D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects / Q000302:isolation & purification; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000302:isolation & purification*; D008826:Microbial Sensitivity Tests; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*