In vitro and in vivo analysis of co-electrospun scaffolds made of medical grade poly(epsilon-caprolactone) and porcine collagen.
In this study, a nanofiber mesh made by co-electrospinning medical grade poly(epsilon-caprolactone) and collagen (mPCL/Col) was fabricated and studied. Its mechanical properties and characteristics were analyzed and compared to mPCL meshes. mPCL/Col meshes showed a reduction in strength but an increase in ductility when compared to PCL meshes. In vitro assays revealed that mPCL/Col supported the attachment and proliferation of smooth muscle cells on both sides of the mesh. In vivo studies in the corpus cavernosa of rabbits revealed that the mPCL/Col scaffold used in conjunction with autologous smooth muscle cells resulted in better integration with host tissue when compared to cell free scaffolds. On a cellular level preseeded scaffolds showed a minimized foreign body reaction.
Chen
Z C C
ZC
Department of Obstetrics and Gynecology, National University of Singapore, Singapore.
Ekaputra
A K
AK
Gauthaman
K
K
Adaikan
P G
PG
Yu
H
H
Hutmacher
D W
DW
D000818:Animals; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry*; D004548:Elasticity; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology; D036103:Nanotechnology; D011091:Polyesters / Q000737:chemistry*; D011817:Rabbits; D013552:Swine
D011091:Polyesters; C016240:polycaprolactone; D003094:Collagen