Enhanced microfiltration devices configured with hydrodynamic trapping and a rain drop bypass filtering architecture for microbial cells detection.
Ultra-fine (<1 microm) microfilters are required to effectively trap microbial cells. We designed microfilters featuring a rain drop bypass architecture, which significantly reduces the likelihood of clogging at the cost of limited cell loss. The new rain drop bypass architecture configuration has a substantially lower pressure drop and allows a better efficiency in trapping protozoan cells (Cryptosporidium parvum and Giardia lamblia) in comparison to our previous generation of a microfilter device. A modified version displaying sub-micron filter gaps was adapted to trap and detect bacterial cells (Escherichia coli), through a method of cells labeling, which aims to amplify the fluorescence signal emission and therefore the sensitivity of detection.
Lay
Christophe
C
Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), Singapore.
Teo
Cheng Yong
CY
Zhu
Liang
L
Peh
Xue Li
XL
Ji
Hong Miao
HM
Chew
Bi-Rong
BR
Murthy
Ramana
R
Feng
Han Hua
HH
Liu
Wen-Tso
WT
D000818:Animals; D016785:Cryptosporidium parvum / Q000166:cytology / Q000276:immunology / Q000302:isolation & purification*; D004867:Equipment Design; D004926:Escherichia coli / Q000166:cytology / Q000276:immunology / Q000302:isolation & purification*; D005453:Fluorescence; D019084:Fluorescent Antibody Technique, Indirect / Q000295:instrumentation / Q000379:methods*; D015200:Fluoroimmunoassay / Q000295:instrumentation / Q000379:methods; D016829:Giardia lamblia / Q000166:cytology / Q000276:immunology / Q000302:isolation & purification*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D012680:Sensitivity and Specificity