Under-expression of CD24 in pre-eclamptic placental tissues determined by quantitative real-time RT-PCR.
BACKGROUND
Pre-eclampsia is a pregnancy-related disorder present in about 5-7% of all pregnancies. CD24 expression was recently reported in different diseases, while it has not yet been determined in pre-eclamptic placental tissues.


METHODS
We collected placental tissues from pre-eclamptic (n = 16) and healthy pregnancies (n = 16). We used the quantitative real-time PCR method with a primer-probe system for determination of CD24 gene expression.


RESULTS
We measured CD24 concentrations of 18.94 +/- 26.86 ng/microl in the pre-eclamptic and 53.85 +/- 92.05 ng/microl in the healthy placental tissues (p = 0.03).


CONCLUSIONS
The quantitative real-time PCR method is suitable to determine CD24 expression in placental tissues. We suppose the low expression of CD24 may cause the enhanced immune reaction and could play a role in the abnormal development of placenta in pre-eclampsia.
Nagy
Bálint
B
1st Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary. nabal@noi1.sote.hu
Berkes
Eniko
E
Rigó
Barbara
B
Bán
Zoltán
Z
Papp
Zoltán
Z
Hupuczi
Petronella
P
D000328:Adult; D051927:CD24 Antigen / Q000235:genetics*; D016022:Case-Control Studies; D005260:Female; D015870:Gene Expression; D017359:HELLP Syndrome / Q000235:genetics / Q000276:immunology; D006801:Humans; D010920:Placenta / Q000276:immunology*; D011225:Pre-Eclampsia / Q000235:genetics* / Q000276:immunology*; D011247:Pregnancy; D012313:RNA / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D051927:CD24 Antigen; C497889:CD24 protein, human; D012313:RNA