title: [Protection of vascular endothelial cells from ox-LDL induced injury by protocatechualdehyde].
abstract: OBJECTIVE
  To observe the protective effects of protocatechualdehyde on the human umbilical vein endothelial cells (CRL-1730) induced injury by ox-LDL.
  
  
  METHODS
  The CRL-1730 were induced injury by ox-LDL, and then treated with protocatechualdehydes for 24 hours. The cytoactive of CRL-1730 was assessed by colorimetry of MTr, the NO level and NOS activity in the cell culture fluid were observed by colorimetry, and the expression of CD40 protein was determined by flow cytometry.
  
  
  RESULTS
  Compared with the ox-LDL group, protocatechualdehyde increased,the number of CRL-1730 and the level of NO and NOS in cell culture fluid. Besides, protocatechualdehyde decreased the expression of CD40 protein, which was increased by ox-LDL.
  
  
  CONCLUSIONS
  Protocatechualdehyde has protective effect on the CRL-1730 induced injury by ox-LDL and its mechanism of action may be related to the CD40/CD40L pathway.
authors.affiliation: Xián Jiaotong University, China.
authors.forename: Chun-jie
authors.initials: CJ
authors.lastname: Han
authors.forename: Rong
authors.initials: R
authors.lastname: Lin
authors.forename: Jun-tian
authors.initials: JT
authors.lastname: Liu
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
mesh_terms: D001161:Arteriosclerosis / Q000517:prevention & control; D019013:CD40 Antigens / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D008077:Lipoproteins, LDL; D008148:Lovastatin / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D027561:Salvia miltiorrhiza / Q000737:chemistry; D015398:Signal Transduction; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D019013:CD40 Antigens; D002396:Catechols; D008077:Lipoproteins, LDL; D020011:Protective Agents; C099252:oxidized low density lipoprotein; D009569:Nitric Oxide; D008148:Lovastatin; D019001:Nitric Oxide Synthase