Single-nucleotide polymorphism genotyping using microarrays.
This unit provides a set of protocols for high throughput SNP genotyping using oligonucleotide microarrays. The basic protocol utilizes allele-specific hybridization in which the microarray provides both the allele discrimination and read-out. In the alternate approach, the allele discrimination is accomplished in solution and then the array is used to provide the read-out of the genotyping reaction. Single nucleotide polymorphisms (SNPs) offer several advantages over other genetic markers including their high density in the.
Patil
N
N
Affymetrix, Santa Clara, California, USA.
Nouri
N
N
McAllister
L
L
Matsukaki
H
H
Ryder
T
T
D000483:Alleles; D005826:Genetics, Medical; D005838:Genotype; D006801:Humans; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D020641:Polymorphism, Single Nucleotide