title: IL-12 signaling drives CD8+ T cell IFN-gamma production and differentiation of KLRG1+ effector subpopulations during Toxoplasma gondii Infection.
abstract: IFN-gamma-producing CD8(+) T lymphocytes are essential effector cells that mediate protective immunity during murine toxoplasmosis, and yet their effector development remains poorly characterized. Vaccination with the carbamoyl phosphate synthase (CPS) mutant strain of Toxoplasma gondii was used to examine the CD8(+) T cell response in the peritoneal effector site. Four CTL subpopulations with varying effector potentials were defined based on the expression of effector molecules and the cell surface activation markers CD62L and killer cell lectin-like receptor G1 (KLRG1). Further phenotypic analysis revealed that the acquisition of KLRG1 among effector subpopulations correlated with the down-regulation of both IL-7R and CD27, suggesting that KLRG1 marks dominant, end-stage effector cells. Using gene-targeted mice, we tested the in vivo requirements of key IL-12 signaling components for effector CTL differentiation. Contrary to established models of viral and bacterial infection, CD8(+) T cell-intrinsic IL-12 signaling was required for the generation of IFN-gamma-producing CTLs in response to T. gondii. Importantly, the development of the KLRG1(+) effector subpopulations, but not the memory precursor-containing KLRG1(-) effector subset, was critically reliant on IL-12. Furthermore, IL-12 signaling-dependent T-bet expression was also found to be important for differentiation of KLRG1(+) effectors. Our results underscore a vital role for IL-12 in not only the induction of IFN-gamma expression but also in the development of heterogeneous subpopulations of effector CD8(+) T cells generated in response to the intracellular parasite T. gondii.
authors.affiliation: Department of Molecular Microbiology and Immunology, Brown University, Providence, RI 02912, USA.
authors.forename: Douglas C
authors.initials: DC
authors.lastname: Wilson
authors.forename: Suzanne
authors.initials: S
authors.lastname: Matthews
authors.forename: George S
authors.initials: GS
authors.lastname: Yap
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism / Q000469:parasitology; D019731:Carbon-Nitrogen Ligases / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology*; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D019041:L-Selectin / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D037181:Lectins, C-Type; D051379:Mice; D018345:Mice, Knockout; D009154:Mutation; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016052:Protozoan Vaccines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D011971:Receptors, Immunologic / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D020395:Receptors, Interleukin-7 / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D014122:Toxoplasma / Q000201:enzymology / Q000276:immunology*; D014124:Toxoplasmosis, Animal / Q000201:enzymology / Q000235:genetics / Q000276:immunology*; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: C451496:Klrg1 protein, mouse; D037181:Lectins, C-Type; D015800:Protozoan Proteins; D016052:Protozoan Vaccines; D011971:Receptors, Immunologic; D020395:Receptors, Interleukin-7; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D019041:L-Selectin; D018664:Interleukin-12; D007371:Interferon-gamma; D019731:Carbon-Nitrogen Ligases