Optical methods.
Equilibrium optical melting curves are used in this unit to determine thermodynamic parameters of nucleic acid complex formation. This section contains all of the relevant equations and a discussion of which are most appropriate to a given situation. Additionally, procedures are given for making preliminary determinations of molar extinction coefficients and for determining the number of oligonucleotides in a complex. The section on extinction coefficients is particularly essential to anyone needing to know solution concentrations.
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Rutgers University, Piscataway, New Jersey, USA.
D001671:Biochemistry / Q000379:methods*; D009691:Nucleic Acid Denaturation; D009841:Oligonucleotides / Q000737:chemistry; D055095:Optics and Photonics; D010710:Phosphates / Q000032:analysis; D012015:Reference Standards; D013816:Thermodynamics
D009841:Oligonucleotides; D010710:Phosphates