title: Quantitation of DNA and RNA with absorption and fluorescence spectroscopy.
abstract: Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers using molecular biology techniques. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques are presented in this appendix. These three procedures cover a range from 5 to 10 ng DNA/ml to 50mg DNA/ml.
authors.affiliation: Hoefer Scientific Instruments, San Francisco, California, USA.
authors.forename: S R
authors.initials: SR
authors.lastname: Gallagher
mesh_terms: D000818:Animals; D006690:Bisbenzimidazole / Q000737:chemistry; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry; D012313:RNA / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D012313:RNA; D004247:DNA; C427203:calf thymus DNA; D006690:Bisbenzimidazole