A synthetic Escherichia coli predator-prey ecosystem.
We have constructed a synthetic ecosystem consisting of two Escherichia coli populations, which communicate bi-directionally through quorum sensing and regulate each other's gene expression and survival via engineered gene circuits. Our synthetic ecosystem resembles canonical predator-prey systems in terms of logic and dynamics. The predator cells kill the prey by inducing expression of a killer protein in the prey, while the prey rescue the predators by eliciting expression of an antidote protein in the predator. Extinction, coexistence and oscillatory dynamics of the predator and prey populations are possible depending on the operating conditions as experimentally validated by long-term culturing of the system in microchemostats. A simple mathematical model is developed to capture these system dynamics. Coherent interplay between experiments and mathematical analysis enables exploration of the dynamics of interacting populations in a predictable manner.
Balagaddé
Frederick K
FK
Department of Bioengineering, Stanford University and Howard Hughes Medical Institute, Stanford, CA, USA.
Song
Hao
H
Ozaki
Jun
J
Collins
Cynthia H
CH
Barnet
Matthew
M
Arnold
Frances H
FH
Quake
Stephen R
SR
You
Lingchong
L
D003198:Computer Simulation; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D017753:Ecosystem; D004926:Escherichia coli / Q000378:metabolism / Q000502:physiology*; D044085:Microfluidics; D008954:Models, Biological; D008962:Models, Theoretical; D009991:Oscillometry; D010957:Plasmids / Q000378:metabolism; D011157:Population Dynamics; D011158:Population Growth; D049490:Systems Biology; D013997:Time Factors
D004129:Dimethylpolysiloxanes; C013830:baysilon