Animal models using lymphocytic choriomeningitis virus.
This unit includes protocols for inducing systemic infection and persistent infection of mice with lymphocytic choriomeningitis virus (LCMV). Methods used to measure T cell responses to LCMV are then described. A protocol to assess anti-LCMV immunity in vivo is also included. Support protocols for preparing LCMV stocks and measuring LCMV titers using a plaque assay are also included. Finally, a support protocol for detecting anti-LCMV antibodies by ELISA is presented.
von Herrath
M
M
The Scripps Research Institute, La Jolla, California, USA.
Whitton
J L
JL
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D004195:Disease Models, Animal; D008213:Lymphocyte Activation; D008216:Lymphocytic Choriomeningitis / Q000097:blood* / Q000821:virology*; D008217:Lymphocytic choriomeningitis virus / Q000254:growth & development / Q000276:immunology / Q000302:isolation & purification; D051379:Mice; D013601:T-Lymphocytes / Q000276:immunology; D010948:Viral Plaque Assay; D014776:Virus Cultivation
D000914:Antibodies, Viral