Soybean defense responses to the soybean aphid.
Transcript profiles in aphid (Aphis glycines)-resistant (cv. Dowling) and -susceptible (cv. Williams 82) soybean (Glycine max) cultivars using soybean cDNA microarrays were investigated. Large-scale soybean cDNA microarrays representing approx. 18 000 genes or c. 30% of the soybean genome were compared at 6 and 12 h post-application of aphids. In a separate experiment utilizing clip cages, expression of three defense-related genes were examined at 6, 12, 24, 48, and 72 h in both cultivars by quantitative real-time PCR. One hundred and forty genes showed specific responses for resistance; these included genes related to cell wall, defense, DNA/RNA, secondary metabolism, signaling and other processes. When an extended time period of sampling was investigated, earlier and greater induction of three defense-related genes was observed in the resistant cultivar; however, the induction declined after 24 or 48 h in the resistant cultivar but continued to increase in the susceptible cultivar after 24 h. Aphid-challenged resistant plants showed rapid differential gene expression patterns similar to the incompatible response induced by avirulent Pseudomonas syringae. Five genes were identified as differentially expressed between the two genotypes in the absence of aphids.
Li
Yan
Y
Physiological and Molecular Plant Biology Program, University of Illinois at Urbana-Champaign, IL, USA.
Zou
Jijun
J
Department of Crop Sciences, University of Illinois at Urbana-Champaign, IL, USA.
Li
Min
M
Department of Crop Sciences, University of Illinois at Urbana-Champaign, IL, USA.
Bilgin
Damla D
DD
Institute for Genomic Biology, University of Illinois at Urbana-Champaign, IL, USA.
Vodkin
Lila O
LO
Department of Crop Sciences, University of Illinois at Urbana-Champaign, IL, USA.
Hartman
Glen L
GL
Department of Crop Sciences, University of Illinois at Urbana-Champaign, IL, USA.|USDA-ARS and National Soybean Research Center, Urbana, IL, USA.
Clough
Steven J
SJ
Department of Crop Sciences, University of Illinois at Urbana-Champaign, IL, USA.|Institute for Genomic Biology, University of Illinois at Urbana-Champaign, IL, USA.|USDA-ARS and National Soybean Research Center, Urbana, IL, USA.
D000818:Animals; D001042:Aphids / Q000502:physiology*; D005247:Feeding Behavior; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005838:Genotype; D007113:Immunity, Innate / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D044224:Pseudomonas syringae / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013025:Glycine max / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology
D012333:RNA, Messenger