title: Single tube optimisation of APOE genotyping based on melting curve analysis.
abstract: OBJECTIVE
  To develop and validate a single-tube protocol for APOE genotyping using fluorescent probes.
  
  
  METHODS
  We have designed and validated a hybrid, single-tube, SimpleProbe/FRET probe protocol that takes advantage of the different probe wavelength emissions.
  
  
  RESULTS
  Our method offers high quality outcomes, minimum interferences between probe signals and is 100% concordant with the reference protocol.
  
  
  CONCLUSIONS
  This method is cheaper, faster and more reliable and versatile than other alternatives proposed.
authors.affiliation: Genetic and Molecular Epidemiology Unit, Department of Preventive Medicine, School of Medicine. University of Valencia, Valencia, Spain. francesc.frances@uv.es
authors.forename: Francesc
authors.initials: F
authors.lastname: Francés
authors.forename: Olga
authors.initials: O
authors.lastname: Portolés
authors.forename: José Vicente
authors.initials: JV
authors.lastname: Sorlí
authors.forename: Marisa
authors.initials: M
authors.lastname: Guillén
authors.forename: José I
authors.initials: JI
authors.lastname: González
authors.forename: Dolores
authors.initials: D
authors.lastname: Corella
mesh_terms: D001057:Apolipoproteins E / Q000097:blood / Q000235:genetics*; D002318:Cardiovascular Diseases / Q000097:blood / Q000235:genetics; D017931:DNA Primers; D005838:Genotype; D006801:Humans; D016133:Polymerase Chain Reaction; D013816:Thermodynamics
chemical_list: D001057:Apolipoproteins E; D017931:DNA Primers