Large-scale purification of latex bead phagosomes from mouse macrophage cell lines and subsequent preparation for high-throughput quantitative proteomics.
Phagocytosis involves the engagement of a diverse array of cell surface receptors whose signal must be integrated on the membrane of the forming phagosomal cup. This method enables the quantitative proteomic analysis of phagosome fractions derived from phagocytes stimulated under two different conditions, thus allowing the complexity of phagosomal signaling to be analyzed in terms of the quantitative changes in phagosomal fraction protein content.
Rupper
Adam
A
Department of Microbiology and Immunology, Louisiana State University Health Sciences Center, Shreveport, LA, USA.
Cardelli
James
J
D000818:Animals; D002460:Cell Line; D002853:Chromatography, Liquid; D008264:Macrophages / Q000378:metabolism*; D051379:Mice; D010588:Phagosomes / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D053719:Tandem Mass Spectrometry