Iodination of soluble and membrane-bound proteins.
Coupling of radioactive (125)I to soluble proteins is a critical step in a number of immunoassays. Chloramine T-catalyzed iodination of soluble proteins, such as immunoglobulins, is described in this unit. Protocols using lactoperoxidase-catalyzed iodination of soluble or membrane-bound proteins are also described.
Samelson
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National Institute of Child Health and Human Development, Bethesda, Maryland.
D002384:Catalysis; D002700:Chloramines / Q000737:chemistry; D054879:Halogenation / Q000276:immunology*; D007136:Immunoglobulins / Q000737:chemistry* / Q000276:immunology; D007457:Iodine Radioisotopes / Q000737:chemistry; D007784:Lactoperoxidase / Q000737:chemistry; D008565:Membrane Proteins / Q000737:chemistry* / Q000276:immunology; D012995:Solubility; D013194:Staining and Labeling; D014105:Tosyl Compounds / Q000737:chemistry
D002700:Chloramines; D007136:Immunoglobulins; D007457:Iodine Radioisotopes; D008565:Membrane Proteins; D014105:Tosyl Compounds; C016300:chloramine-T; D007784:Lactoperoxidase