Sesame oil does not show accumulatively enhanced protection against oxidative stress-associated hepatic injury in septic rats.
BACKGROUND
Sepsis is one of the major causes of death reported in intensive care units. A daily supplement of sesame oil for 1 week significantly attenuates oxidative stress-associated hepatic injury in septic rats. However, the excess intake of sesame oil may be associated with a health risk. This study investigates the effect of accumulative sesame oil on oxidative stress-associated hepatic injury after cecal ligation and puncture in rats.


METHODS
Sesame oil was administered daily (4 mL/kg/d, orally) to rats, and the total intake of sesame oil ranged from 0 (control) to 140 mL/kg before cecal ligation and puncture in 9 groups of rats. Oxidative stress was examined by determining the levels of lipid peroxidation and glutathione. Hepatic injury was evaluated by measuring serum levels of aspartate aminotransferase and alkaline phosphatase.


RESULTS
Rats that received sesame oil for 4 and 5 weeks had a lower body weight gain compared with those that received saline. Lipid peroxidation was decreased in the 20-mL/kg and 28-mL/kg groups, but it was increased in the 140-mL/kg group compared with the control group. Glutathione levels were increased in the < or =28-mL/kg groups compared with the control group. Serum levels of aspartate aminotransferase and alkaline phosphatase were reduced in the < or =28-mL/kg groups compared with the control group.


CONCLUSIONS
Sesame oil does not demonstrate accumulatively enhanced protection against oxidative stress-associated hepatic injury after cecal ligation and puncture in rats.
Hsu
Dur-Zong
DZ
Department of Environmental and Occupational Health, National Cheng Kung University Medical College, Tainan, Taiwan.
Chien
Se-Ping
SP
Li
Ya-Hui
YH
Liu
Ming-Yie
MY
D000818:Animals; D002432:Cecum / Q000293:injuries* / Q000601:surgery; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000201:enzymology / Q000293:injuries*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis; D012715:Sesame Oil / Q000494:pharmacology*; D013047:Specific Pathogen-Free Organisms; D015430:Weight Gain
D012715:Sesame Oil; D005978:Glutathione