Measurement of polyclonal immunoglobulin synthesis using ELISA.
The enzyme-linked immunosorbent assay (ELISA) is a simple, specific, highly reproducible, and nonradioactive method for measuring immunoglobulin levels in cell culture supernatants and other biologic fluids. It can be used to measure the common immunoglobulin classes (IgG, IgM, and IgA) as described in the basic protocol, as well as classes and subclasses found in low levels in these fluids (IgG4 and IgE), as detailed in the alternate protocols.
Nutman
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National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006801:Humans; D007132:Immunoglobulin Isotypes / Q000032:analysis / Q000096:biosynthesis*; D007136:Immunoglobulins / Q000032:analysis / Q000096:biosynthesis*
D007132:Immunoglobulin Isotypes; D007136:Immunoglobulins