Synthesis and ITC characterization of novel nanoparticles constituted by poly(gamma-benzyl L-glutamate)-beta-cyclodextrin.
Imparting desired technological characteristics to polymeric nanoparticles requires the development of original polymers. In the present work, the synthesis and characterization of a novel PBLG-derivative, the poly(gamma-benzyl L-glutamate)-beta-cyclodextrin (PBLG-beta-CD-50), have been carried out. Nanoparticles from either PBLG-beta-CD-50 polymer or from mixtures with PBLG have been prepared using a modified nanoprecipitation method. Spherically shaped nanoparticles with diameter in the range of 50-70 nm were obtained, as determined by dynamic laser light scattering and transmission electron microscopy. The presence of a surfactant in the suspension medium had almost no influence on these parameters and was not necessary to the shelf-stability of the suspension. Further, isothermal titration microcalorimetry (ITC) experiments have been used to show unambiguously that about 20% of the cyclodextrins remain functional within the particles. Consequently, this system may be of interest when association of large amounts of hydrophobic drugs to nanoparticles is required.
Barbosa
Ma Elisa Martínez
ME
UMR CNRS 8612, Laboratoire de Pharmacotechnie et Biopharmacie, Faculté de Pharmacie, Université Paris-Sud, 5 rue J. B. Clément, 92290 Châtenay-Malabry, France.
Bouteiller
Laurent
L
Cammas-Marion
Sandrine
S
Montembault
Véronique
V
Fontaine
Laurent
L
Ponchel
Gilles
G
D000547:Amantadine / Q000737:chemistry; D002151:Calorimetry / Q000379:methods*; D011232:Chemical Precipitation; D006358:Hot Temperature; D008968:Molecular Conformation; D053758:Nanoparticles / Q000032:analysis* / Q000737:chemistry* / Q000648:ultrastructure; D020442:Poloxamer / Q000737:chemistry; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000138:chemical synthesis / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D047392:beta-Cyclodextrins / Q000032:analysis* / Q000138:chemical synthesis* / Q000737:chemistry
D047392:beta-Cyclodextrins; C532265:poly(gamma-benzyl glutamate)-beta-cyclodextrin; D020442:Poloxamer; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D000547:Amantadine