[Protection of vascular endothelial cells from ox-LDL induced injury by protocatechualdehyde].
OBJECTIVE
To observe the protective effects of protocatechualdehyde on the human umbilical vein endothelial cells (CRL-1730) induced injury by ox-LDL.


METHODS
The CRL-1730 were induced injury by ox-LDL, and then treated with protocatechualdehydes for 24 hours. The cytoactive of CRL-1730 was assessed by colorimetry of MTr, the NO level and NOS activity in the cell culture fluid were observed by colorimetry, and the expression of CD40 protein was determined by flow cytometry.


RESULTS
Compared with the ox-LDL group, protocatechualdehyde increased,the number of CRL-1730 and the level of NO and NOS in cell culture fluid. Besides, protocatechualdehyde decreased the expression of CD40 protein, which was increased by ox-LDL.


CONCLUSIONS
Protocatechualdehyde has protective effect on the CRL-1730 induced injury by ox-LDL and its mechanism of action may be related to the CD40/CD40L pathway.
Han
Chun-jie
CJ
Xián Jiaotong University, China.
Lin
Rong
R
Liu
Jun-tian
JT
Liu
Yu
Y
Zhang
Hui
H
D001161:Arteriosclerosis / Q000517:prevention & control; D019013:CD40 Antigens / Q000378:metabolism; D002396:Catechols / Q000494:pharmacology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology / Q000473:pathology*; D005434:Flow Cytometry; D006801:Humans; D008077:Lipoproteins, LDL; D008148:Lovastatin / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D027561:Salvia miltiorrhiza / Q000737:chemistry; D015398:Signal Transduction; D014471:Umbilical Veins / Q000166:cytology
D019013:CD40 Antigens; D002396:Catechols; D008077:Lipoproteins, LDL; D020011:Protective Agents; C099252:oxidized low density lipoprotein; D009569:Nitric Oxide; D008148:Lovastatin; D019001:Nitric Oxide Synthase