Flow cytometry of bacterial membrane potential and permeability.
This chapter describes reliable flow cytometric methods for assessment of two important physiologic characteristics of bacteria, membrane potential and membrane permeability, which can provide indications of the effects of antimicrobial agents on microorganisms.
Shapiro
Howard M
HM
The Center for Microbial Cytometry and Howard M Shapiro, West Newton, MA, USA.
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D018407:Bacterial Physiological Phenomena / Q000187:drug effects; D002232:Carbocyanines / Q000378:metabolism; D002463:Cell Membrane Permeability / Q000187:drug effects*; D005434:Flow Cytometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000378:metabolism; D008564:Membrane Potentials / Q000187:drug effects*; D020102:Mycobacterium smegmatis / Q000187:drug effects / Q000502:physiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000502:physiology
D000900:Anti-Bacterial Agents; D002232:Carbocyanines; D005456:Fluorescent Dyes; C098830:TO-PRO-3; C081554:3,3'-diethyloxacarbocyanine