[The effect of aloesin on melanocytes in the pigmented skin equivalent model].
OBJECTIVE
To observe the effect of aloesin, tea polyphenols, arbutin on melanocytes in the pigmented skin equivalent model.


METHODS
First, we constructed the pigmented skin equivalent model in vitro. And then we detected the effect of aloesin, tea polyphenols and arbutin on the cells' shape, tyrosinase activity and formation of melanin in the constructed pigmented skin equivalent.


RESULTS
Three depigmenting agents showed an inhibition effect on the tyrosinase activity of melanocytes and reduced significantly melanin content in the pigmented skin equivalent model, in which the tea polyphenols had the strongest effect, and then was the aloesin. But the tea polyphenols showed the strongest toxicity, while the aloesin and arbutin had a much lower toxicity.


CONCLUSIONS
All the three depigmenting agents showed a concentration dependent suppression effect on the tyrosinase activity and formation of melanin, in which the tea polyphenols was the strongest effect( P <0.05). Aoesin has a good suppression effect on the tyrosinase activity and formation of melanin, but has a much lower toxicity, which could be used as a safe depigmenting agent.
Yang
Zhuang-qun
ZQ
Department of Oral and Maxillofacial Plastic Surgery, Stomatological College, Xi'an Jiao Tong University, Xi'an 710004, China. yzhqun@mail.xjtu.edu.cn
Wang
Zheng-Hui
ZH
Zhang
Tie-Liang
TL
Tu
Jun-Bo
JB
Song
Yong
Y
Hu
Xiao-Yi
XY
Li
Guo-Guang
GG
D001104:Arbutin / Q000494:pharmacology*; D002478:Cells, Cultured; D002867:Chromones / Q000494:pharmacology*; D005419:Flavonoids / Q000494:pharmacology*; D052816:Foreskin / Q000166:cytology; D005960:Glucosides / Q000494:pharmacology*; D006801:Humans; D008297:Male; D008543:Melanins / Q000096:biosynthesis; D008544:Melanocytes / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D010858:Pigmentation; D059808:Polyphenols; D012867:Skin / Q000187:drug effects; D015595:Skin Aging / Q000187:drug effects
D002867:Chromones; D005419:Flavonoids; D005960:Glucosides; D008543:Melanins; D010636:Phenols; D059808:Polyphenols; D001104:Arbutin; C069868:aloesin