Correlation between DNA damage and sperm parameters: a prospective study of 1,633 patients.
OBJECTIVE
To investigate DNA fragmentation by using terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling in relation to World Health Organization parameters and computer-aided sperm analysis (CASA) in sperm to determine the possibility of obtaining a correlation among CASA parameters, sperm morphology, and DNA fragmentation.


METHODS
Sperm analysis according to World Health Organization parameters, terminal deoxyribonucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) for sperm DNA fragmentation, and CASA for sperm movement. Prospective study.


METHODS
All the patients were under clinical management, consulting for hypofertility at a fertility center in France.


METHODS
One thousand six hundred thirty-three men who were referred for infertility investigation, including a complete sperm analysis.


METHODS
Sperm analysis and DNA damage testing.


METHODS
Sperm morphology, DNA fragmentation, and movement characteristics.


RESULTS
One third of the patients had a TUNEL rate of >30%. Analysis of the 21 semen parameters tested revealed that 7 of them were significantly correlated with the TUNEL results.


CONCLUSIONS
World Health Organization sperm parameters and DNA damage are complementary, rather than strongly linked. This should be considered to more fully understand the paternal contribution in assisted reproductive technologies failures.
Cohen-Bacrie
Paul
P
Laboratoire d'Eylau, Paris, France. pcb@eylau.fr
Belloc
Stephanie
S
Ménézo
Yves J R
YJ
Clement
Patrice
P
Hamidi
Jamal
J
Benkhalifa
Moncef
M
D000328:Adult; D004249:DNA Damage; D053938:DNA Fragmentation; D006801:Humans; D020287:In Situ Nick-End Labeling; D008297:Male; D011446:Prospective Studies; D020554:Sperm Injections, Intracytoplasmic; D013081:Sperm Motility; D013094:Spermatozoa / Q000473:pathology*