Multicolor Fluorescence In Situ Hybridization (FISH) approaches for simultaneous analysis of the entire human genome.
The ability to generate chromosome-specific paints and employ combinatorial labeling strategies has made it possible to differentiate all 24 human chromosomes in a single metaphase spread. Such technology is particularly useful when there is a limited number of metaphase spreads for analyses and when interchromosomal rearrangements are ill-defined or very complex. There are three systems currently available for simultaneous FISH analysis of all human chromosomes: spectral karyotyping (SKY), Multiplex FISH (M-FISH), and Rx-FISH. This overview discusses each of these systems and the recent advances which have made them possible.
Lee
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Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Rens
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Yang
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D000818:Animals; D020223:Chromosome Painting / Q000379:methods; D015894:Genome, Human; D023281:Genomics / Q000379:methods; D006801:Humans; D006922:Hylobates / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D008297:Male; D032681:Spectral Karyotyping / Q000379:methods