Oligoribonucleotides with 2'-O-(tert-butyldimethylsilyl) groups.
The chemical synthesis of oligoribonucleotides on solid support is routinely performed via the phosphoramidite method. However, the additional 2-OH function of the ribofuranosyl sugar requires suitable protection during oligoribonucleotide synthesis. This unit describes methods for 2-OH protection using the TBDMS group.
Bellon
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Ribozyme Pharmaceuticals, Boulder, Colorado, USA.
D064753:Ammonium Hydroxide; D001671:Biochemistry / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D000431:Ethanol / Q000737:chemistry; D006878:Hydroxides / Q000737:chemistry; D008744:Methylamines / Q000737:chemistry; D009843:Oligoribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D017646:Organosilicon Compounds / Q000737:chemistry*; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D013997:Time Factors
D006878:Hydroxides; D008744:Methylamines; D009843:Oligoribonucleotides; D017646:Organosilicon Compounds; D000644:Quaternary Ammonium Compounds; C009660:t-butyldimethylsilyl compounds; D000431:Ethanol; D064753:Ammonium Hydroxide; C027451:methylamine; C009405:tetrabutylammonium