title: Whole-mount immunohistochemistry of the brain.
abstract: The gross anatomical distribution of an antigen is typically mapped using a combination of serial sectioning, immunocytochemistry, and three-dimensional reconstruction. This is a tedious and time-consuming procedure, which introduces an array of potential alignment and differential shrinkage errors and requires considerable experience and specialized equipment. In particular, it is unsuited for routine screening applications. To circumvent these problems, this unit presents a routine whole-mount immunocytochemistry protocol that can be used to map many antigenic distributions in the developing and adult brain. The technique can also be easily adapted to detect anterograde and retrograde transport tracers.
authors.affiliation: Konyang University, Chungnam, South Korea.
authors.forename: Se-Hoon
authors.initials: SH
authors.lastname: Kim
authors.forename: Priscilla
authors.initials: P
authors.lastname: Che
authors.forename: Seung-Hyuk
authors.initials: SH
authors.lastname: Chung
authors.forename: Debora
authors.initials: D
authors.lastname: Doorn
authors.forename: Monica
authors.initials: M
authors.lastname: Hoy
authors.forename: Matt
authors.initials: M
authors.lastname: Larouche
authors.forename: Hassan
authors.initials: H
authors.lastname: Marzban
authors.forename: Justyna
authors.initials: J
authors.lastname: Sarna
authors.forename: Sepehr
authors.initials: S
authors.lastname: Zahedi
authors.forename: Richard
authors.initials: R
authors.lastname: Hawkes
mesh_terms: D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives / Q000378:metabolism; D001921:Brain / Q000033:anatomy & histology* / Q000378:metabolism*; D003911:Dextrans / Q000378:metabolism; D016591:Histocytological Preparation Techniques / Q000295:instrumentation / Q000379:methods; D007150:Immunohistochemistry / Q000295:instrumentation / Q000379:methods*; D051379:Mice; D009474:Neurons / Q000378:metabolism
chemical_list: D003911:Dextrans; C076397:biotinylated dextran amine; D001710:Biotin