title: Nanomosaic network for the detection of proteins without direct electrical contact.
abstract: A nanomosaic network of metallic nanoparticles for the detection of ultralow concentrations of proteins is reported, which uses two planar microelectrodes embedded in a microchip that permit generation of capacitive coupling to the nanomosaic system without the need for direct electrical contact with the channel. By tailoring the microchannel surface using a sandwich configuration of polyethylene terephthalate/gold nanoparticles/poly(L-lysine), the surface charge can be modified following biomolecular interactions and monitored using a noncontact admittance technique. This nanodevice system behaves like a tunable capacitor and can be employed for the detection of any kind of molecule. The femtomolar detection of an anionic protein, such as beta-lactoglobulin in phosphate-buffered saline medium, is taken as an example.
authors.affiliation: Laboratoire des Interfaces et Systèmes Electrochimiques, UPR15 du CNRS, niversité Pierre et Marie Curie, Paris VI, 75252 Paris, France. gamby@ccr.jussieu.fr
authors.forename: Jean
authors.initials: J
authors.lastname: Gamby
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Abid
authors.forename: Bernard
authors.initials: B
authors.lastname: Tribollet
authors.forename: Hubert H
authors.initials: HH
authors.lastname: Girault
mesh_terms: D004563:Electrochemistry; D008855:Microscopy, Electron, Scanning; D036103:Nanotechnology; D011506:Proteins / Q000032:analysis*; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D011506:Proteins