Mouse monoclonal antibodies in biological research: strategies for high-throughput production.
Mouse monoclonal antibodies have become key components in basic research as well as in the clinical laboratory. Being invaluable tools in many biological assays, they continue to be the primary choice in the research field, although the conventional technology used for hybridoma generation and screening is a still lengthy, time-consuming and low-throughput process. With the advent of genetic immunisation and the application of automation and microarray to the traditional biological assays, the monoclonal antibody field has been revolutionised. Here, we will briefly review the most relevant strategies which have made the manufacture of murine monoclonal antibodies a faster and high-throughput technology.
Chiarella
Pieranna
P
Laboratory of Molecular Medicine and Biotechnology, CIR, Campus Bio-Medico University of Rome, Via Alvaro del Portillo 21, 00128 Rome, Italy. p.chiarella@unicampus.it
Fazio
Vito Michele
VM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis*; D001695:Biology / Q000379:methods*; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D051379:Mice; D040081:Protein Array Analysis; D012106:Research
D000911:Antibodies, Monoclonal