Measurement of interleukin-13.
This unit describes two protocols that can be used to quantitate interleukin-13 (IL-13). The enzyme-linked immunosorbent assay (ELISA) has the advantage of being highly specific for human IL-13 and does not recognize other cytokines present in the sample. A bioassay is also presented based on the ability of IL-13 to stimulate the growth of the B9 plasmacytoma cell line. The bioassay method can be used to detect both mouse and human IL-13. B9 cells are dependent on IL-6 for growth and will also respond to IL-4. Thus, although B9 cells can readily be used to quantify IL-13 in the absence of other cytokines, neutralizing antibodies must be incorporated into the bioassay when other cytokines are present in the sample.
Lauder
Angus
A
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
McKenzie
Andrew N J
ANJ
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
D000818:Animals; D001681:Biological Assay; D045744:Cell Line, Tumor; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D006801:Humans; D018793:Interleukin-13 / Q000032:analysis*; D051379:Mice
D018793:Interleukin-13