Ex vivo and in vivo gene delivery to the brain.
This unit describes methods for grafting genetically modified cells for ex vivo delivery of specific genes into the rat brain and direct delivery of transgenes to brain cells in vivo using recombinant viral vectors. These methods assess the function of a gene in the brain. The ex vivo approach of gene transfer to the nervous system depends on genetic manipulation of cells in vitro prior to grafting of the cells into the brain to enable production of a transgene at physiologically significant levels for a long period of time. This unit also includes procedures for transcardial perfusion to fix the tissue prior to analysis of expression and for sectioning of brains by use of a freezing sledge microtome. Thionine staining of tissue sections is also described.
Suhonen
Jaana
J
The Salk Institute, La Jolla, California, USA.
Ray
Jasodhara
J
Blömer
Ulrike
U
Gage
Fred H
FH
Kaspar
Brian
B
D000818:Animals; D001921:Brain / Q000378:metabolism*; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*