Purification of T cell subpopulations.
This unit describes a procedure for isolating T cell populations or subpopulations using the method of indirect panning. In this method, cells are selected by their capacity to bind to antibody-coated plates on the basis of particular cell-surface markers. It is superior to the antibody/complement lysis method (also presented) because the nonselected cell population can be retrieved.
Kanof
M E
ME
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.
D000818:Animals; D002469:Cell Separation / Q000379:methods*; D006801:Humans; D016176:T-Lymphocyte Subsets / Q000166:cytology*