Blocking of bacterial biofilm formation by a fish protein coating.
Bacterial biofilm formation on inert surfaces is a significant health and economic problem in a wide range of environmental, industrial, and medical areas. Bacterial adhesion is generally a prerequisite for this colonization process and, thus, represents an attractive target for the development of biofilm-preventive measures. We have previously found that the preconditioning of several different inert materials with an aqueous fish muscle extract, composed primarily of fish muscle alpha-tropomyosin, significantly discourages bacterial attachment and adhesion to these surfaces. Here, this proteinaceous coating is characterized with regards to its biofilm-reducing properties by using a range of urinary tract infectious isolates with various pathogenic and adhesive properties. The antiadhesive coating significantly reduced or delayed biofilm formation by all these isolates under every condition examined. The biofilm-reducing activity did, however, vary depending on the substratum physicochemical characteristics and the environmental conditions studied. These data illustrate the importance of protein conditioning layers with respect to bacterial biofilm formation and suggest that antiadhesive proteins may offer an attractive measure for reducing or delaying biofilm-associated infections.
Vejborg
Rebecca Munk
RM
Microbial Adhesion Group, Technical University of Denmark, DK-2800 Lyngby, Denmark.
Klemm
Per
P
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001422:Bacterial Adhesion / Q000187:drug effects; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D005260:Female; D029941:Fish Proteins / Q000494:pharmacology*; D005399:Fishes; D006801:Humans; D007709:Klebsiella / Q000187:drug effects* / Q000254:growth & development / Q000302:isolation & purification; D014552:Urinary Tract Infections / Q000382:microbiology
D000900:Anti-Bacterial Agents; D029941:Fish Proteins