High-throughput screening for protein-protein interactions using yeast two-hybrid arrays.
Arrays are used for parallel assays of large numbers of proteins in a single experiment. They provide an alternative to libraries for protein screening purposes. This unit describes a protein array composed of living yeast (Saccharomyces cerevisiae) cells that can be used in a functional screen for protein interactions, the two-hybrid assay. The protocols described here use the yeast proteome as an example, but they can be applied to any other genome or subset thereof.
Cagney
Gerard
G
University of Toronto, Banting and Best Institute of Medical Research, Toronto, Ontario, Canada.|University of Washington, Seattle, Washington.
Uetz
Peter
P
Research Center Karlsruhe, Karlsruhe, Germany.|University of Washington, Seattle, Washington.
D040081:Protein Array Analysis / Q000379:methods*; D011485:Protein Binding; D020543:Proteome / Q000378:metabolism; D012371:Robotics; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D020543:Proteome; D029701:Saccharomyces cerevisiae Proteins