title: Detection and proteomic identification of S-nitrosated proteins in human hepatocytes.
abstract: The S-nitrosation of protein thiols is a redox-based posttranslational modification that modulates protein function and cell phenotype. Although the detection of S-nitrosated proteins is problematical because of the lability of S-nitrosothiols, an increasing range of proteins has been shown to undergo S-nitrosation with the improvement of molecular tools. This chapter describes the methodology used to identify potential targets of S-nitrosation in cultured primary human hepatocytes using proteomic approaches. This methodology is based on the biotin switch method, which labels S-nitrosated proteins with an affinity tag, allowing their selective detection and proteomic identification.
authors.affiliation: Liver Research Unit, Hospital Universitario Reina Sofía, Córdoba, Spain.
authors.forename: Laura M
authors.initials: LM
authors.lastname: López-Sánchez
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Corrales
authors.forename: Manuel
authors.initials: M
authors.lastname: De La Mata
authors.forename: Jordi
authors.initials: J
authors.lastname: Muntané
authors.forename: Antonio
authors.initials: A
authors.lastname: Rodríguez-Ariza
mesh_terms: D002478:Cells, Cultured; D022781:Hepatocytes / Q000378:metabolism*; D006801:Humans; D015538:Nitrosation; D011506:Proteins / Q000032:analysis* / Q000145:classification / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D026403:S-Nitrosothiols / Q000032:analysis* / Q000145:classification / Q000378:metabolism*
chemical_list: D011506:Proteins; D026403:S-Nitrosothiols