title: Development of FET-type albumin sensor for diagnosing nephritis.
abstract: An albumin biosensor based on a potentiometric measurement using Biofield-effect-transistor (BioFET) has been designed and fabricated, and its characteristics were investigated. The BioFET was fabricated using semiconductor integrated circuit (IC) technology. The gate surface of the BioFET was chemically modified by newly developed self-assembled monolayer (SAM) synthesized by a thiazole benzo crown ether ethylamine (TBCEA)-thioctic acid to immobilize anti-albumin. SAM formation, antibody immobilization, and antigen-antibody interaction were verified using surface plasmon resonance (SPR). The output voltage changes of the BioFET with respect to various albumin concentrations were obtained. Quasi-reference electrode (QRE) and reference FET (ReFET) has been integrated with the BioFET, and its output characteristic was investigated. The results demonstrate the feasibility of the BioFET as the albumin sensor for diagnosing nephritis.
authors.affiliation: School of Electrical Engineering & Computer Science, Kyungpook National University, 1370 Sankyuk-dong, Buk-gu, Daegu 702-701, Republic of Korea.
authors.forename: Keun-Yong
authors.initials: KY
authors.lastname: Park
authors.forename: Young-Soo
authors.initials: YS
authors.lastname: Sohn
authors.forename: Chang-Kyu
authors.initials: CK
authors.lastname: Kim
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.forename: Young-Seuk
authors.initials: YS
authors.lastname: Bae
authors.forename: Sie-Young
authors.initials: SY
authors.lastname: Choi
mesh_terms: D000418:Albumins / Q000032:analysis*; D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation*; D004563:Electrochemistry / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D009393:Nephritis / Q000097:blood* / Q000175:diagnosis*; D014173:Transistors, Electronic
chemical_list: D000418:Albumins; D015415:Biomarkers