The neuropeptide calcitonin gene-related peptide (CGRP) stimulates T cell migration into collagen matrices.
CGRP significantly stimulated migration of non-activated and anti-CD3 activated T lymphocytes into a collagen matrix when present inside the collagen, whereas somatostatin-14, NPY, substance P, VIP, beta-endorphin and metenkephalin had no or little effect. The CGRP antagonist CGRP 8-37 abrogated the CGRP-induced cell infiltration. Virtually all migrating cells were CD3+ (>96%) and CGRP did not stimulate B-cell migration. The migration capacity showed no selective relationship to the expression of CD4+, CD8+, CD45RO+ (memory), or CD45RA+ (naive) T cell markers indicating that the regulation of T cell migration is distinct from that of the major T cell phenotypes.
Talme
Toomas
T
Department of Medicine, Section of Dermatology and Venereology, Karolinska Institutet at Karolinska University Hospital, Huddinge, Sweden.
Liu
Zhiwen
Z
Sundqvist
Karl-Gösta
KG
D000704:Analysis of Variance; D015703:Antigens, CD / Q000378:metabolism; D015740:Calcitonin Gene-Related Peptide / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects*; D024042:Collagen Type I / Q000502:physiology*; D004347:Drug Interactions; D006801:Humans; D013004:Somatostatin / Q000494:pharmacology; D013601:T-Lymphocytes / Q000187:drug effects*
D015703:Antigens, CD; D024042:Collagen Type I; D013004:Somatostatin; D015740:Calcitonin Gene-Related Peptide