Detection of mutations by denaturing gradient gel electrophoresis.
This unit describes the procedure for determining the melting profile for a given PCR-amplified sequence by perpendicular denaturing gradient gel electrophoresis (DGGE) and, using that information, for developing a screening assay based on either parallel DGGE, CDGE (Constant Denaturant Gel Electrophoresis), or TTGE (Temporal/Temperature Gradient Electrophoresis). Four support protocols describe techniques for pouring perpendicular and parallel denaturing gradient gels, constant denaturant gels, and temporal temperature gradient gels.
Børresen-Dale
A L
AL
The Norwegian Radium Hospital, Oslo, Norway.
Hovig
E
E
Smith-Sørensen
B
B
D004247:DNA / Q000235:genetics / Q000302:isolation & purification; D004252:DNA Mutational Analysis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005826:Genetics, Medical; D006801:Humans; D009691:Nucleic Acid Denaturation
D004247:DNA