title: Detection and isolation of cytokine secreting cells using the cytometric cytokine secretion assay.
abstract: The cell-surface affinity matrix technology, otherwise called "secretion assay" or "capture assay," represents a new, innovative method for the analysis and enrichment of viable cells according to secreted molecules such as antibodies or cytokineof T cells to induce cytokine secretion is included. Alternate protocols provide very rapid and simple procedures for the direct detection of antigen-specific T cells from small numbers of PBMCs or whole blood without magnetic enrichment.
authors.affiliation: Miltenyi Biotec, Bergisch-Gladbach, Germany.
authors.forename: Mario
authors.initials: M
authors.lastname: Assenmacher
authors.affiliation: Deutsches Rheumaforschungszentrum Berlin, Berlin, Germany.
authors.forename: Max
authors.initials: M
authors.lastname: Löhning
authors.affiliation: Deutsches Rheumaforschungszentrum Berlin, Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Radbruch
mesh_terms: D000818:Animals; D000941:Antigens / Q000276:immunology; D001681:Biological Assay / Q000379:methods*; D016207:Cytokines / Q000276:immunology*; D005434:Flow Cytometry; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D000941:Antigens; D016207:Cytokines