Chromatographic deuterium isotope effects of derivatized N-glycans and N-glycopeptides in a zwitterionic type of hydrophilic interaction chromatography.
HPLC/MS is now an essential method for the analysis of glycans and glycopeptides. A technique using ICATs is also becoming popular for their comparative/quantitative analysis based on MS signal intensities. However, the RP HPLC most often used causes "doublet" peaks of deuterium-labeled and nonlabeled peptides, which are well known for causing the chromatographic deuterium isotope effect. Such doublet peaks are undesirable for precise comparison of MS signal intensities. This report shows that, in the zwitterionic type of hydrophilic interaction chromatography, the chromatographic deuterium isotope effect is negligibly small for N-glycans derivatized with deuterium-labeled 2-aminopyridine and N-glycopeptides derivatized with deuterium-labeled succinic anhydride.
Takegawa
Yasuhiro
Y
Graduate School of Advanced Life Science, Hokkaido University, Sapporo, Japan.
Hato
Megumi
M
Deguchi
Kisaburo
K
Nakagawa
Hiroaki
H
Nishimura
Shin-Ichiro
S
D000595:Amino Acid Sequence; D000631:Aminopyridines; D002853:Chromatography, Liquid / Q000379:methods*; D003903:Deuterium; D004563:Electrochemistry; D006020:Glycopeptides / Q000737:chemistry / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013387:Succinic Anhydrides
D000631:Aminopyridines; D006020:Glycopeptides; D011134:Polysaccharides; D013387:Succinic Anhydrides; C031801:succinic anhydride; D003903:Deuterium; C032439:alpha-aminopyridine