title: Two-dimensional electrophoretic comparison of metastatic and non-metastatic human breast tumors using in vitro cultured epithelial cells derived from the cancer tissues.
abstract: BACKGROUND
  Breast carcinomas represent a heterogeneous group of tumors diverse in behavior, outcome, and response to therapy. Identification of proteins resembling the tumor biology can improve the diagnosis, prediction, treatment selection, and targeting of therapy. Since the beginning of the post-genomic era, the focus of molecular biology gradually moved from genomes to proteins and proteomes and to their functionality. Proteomics can potentially capture dynamic changes in protein expression integrating both genetic and epigenetic influences.
  
  
  METHODS
  We prepared primary cultures of epithelial cells from 23 breast cancer tissue samples and performed comparative proteomic analysis. Seven patients developed distant metastases within three-year follow-up. These samples were included into a metastase-positive group, the others formed a metastase-negative group. Two-dimensional electrophoretical (2-DE) gels in pH range 4-7 were prepared. Spot densities in 2-DE protein maps were subjected to statistical analyses (R/maanova package) and data-mining analysis (GUHA). For identification of proteins in selected spots, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed.
  
  
  RESULTS
  Three protein spots were significantly altered between the metastatic and non-metastatic groups. The correlations were proven at the 0.05 significance level. Nucleophosmin was increased in the group with metastases. The levels of 2,3-trans-enoyl-CoA isomerase and glutathione peroxidase 1 were decreased.
  
  
  CONCLUSIONS
  We have performed an extensive proteomic study of mammary epithelial cells from breast cancer patients. We have found differentially expressed proteins between the samples from metastase-positive and metastase-negative patient groups.
authors.affiliation: Department of Oncology, 1st Faculty of Medicine, Charles University Prague, Czech Republic. vydra@medico.cz
authors.forename: Jan
authors.initials: J
authors.lastname: Vydra
authors.forename: Irena
authors.initials: I
authors.lastname: Selicharová
authors.forename: Katerina
authors.initials: K
authors.lastname: Smutná
authors.forename: Miloslav
authors.initials: M
authors.lastname: Sanda
authors.forename: Eva
authors.initials: E
authors.lastname: Matousková
authors.forename: Eva
authors.initials: E
authors.lastname: Bursíková
authors.forename: Markéta
authors.initials: M
authors.lastname: Prchalová
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Velenská
authors.forename: David
authors.initials: D
authors.lastname: Coufal
authors.forename: Jirí
authors.initials: J
authors.lastname: Jirácek
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001943:Breast Neoplasms / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D006801:Humans; D008875:Middle Aged; D009362:Neoplasm Metastasis / Q000473:pathology* / Q000503:physiopathology*; D009363:Neoplasm Proteins / Q000378:metabolism*; D010449:Peptide Mapping; D040901:Proteomics; D014407:Tumor Cells, Cultured
chemical_list: D009363:Neoplasm Proteins