Effects of pyrolytic carbon on postoperative adhesion formation in rats.
BACKGROUND
Peritoneal adhesion is a consequence of wound healing that sometimes causes severe postoperative complications. Despite all the preventive measures and agents, adhesions have still not been eliminated completely. The aim of this study was to evaluate the effects of pyrolytic carbon on the development of postoperative peritoneal and uterine tube adhesions.


METHODS
Twenty female Wistar-Albino type rats were used in this study. The rats were divided into two groups: treatment and control groups. After ketamine anaesthesia (50 mg/kg IM), a lower midline abdominal incision was performed and a standard uterine lesion was created by electrocauterization. In the study group, pyrolytic carbon was applied to the surface of the uterus and no additional procedure was applied in the control group. After 2 weeks, all of the rats were killed and uterine adhesions were staged according to the Leach scoring system.


RESULTS
In the control group, the total adhesion score was 8.8 +/- 0.42. In the study group, the total adhesion score was 3.3 +/- 1.49, significantly lower than that in the control group (P < 0.001).


CONCLUSIONS
Pyrolytic carbon significantly reduces the prevalence of peritoneal and uterine tube adhesion formation in rats. However, further more detailed investigations are needed before this material is used in clinical practice.
Demirbag
Suzi
S
Department of Pediatric Surgery, Gulhane Military Medical Academy, Etlik, Ankara, Turkey. suzidemirbag@gmail.com
Atabek
Cuneyt
C
Guven
Ahmet
A
Oztas
Emin
E
Surer
Ilhami
I
Ozturk
Haluk
H
Yesildaglar
Narter
N
D000818:Animals; D001672:Biocompatible Materials / Q000008:administration & dosage / Q000494:pharmacology; D002244:Carbon / Q000008:administration & dosage / Q000494:pharmacology*; D005260:Female; D006651:Histocytochemistry; D010532:Peritoneal Diseases / Q000473:pathology / Q000517:prevention & control*; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar; D000267:Tissue Adhesions / Q000517:prevention & control; D014591:Uterine Diseases / Q000473:pathology / Q000517:prevention & control*; D014599:Uterus / Q000601:surgery*; D015040:Zirconium / Q000008:administration & dosage
D001672:Biocompatible Materials; C009139:pyrolytic carbon; D002244:Carbon; D015040:Zirconium; C028541:zirconium oxide