title: Methods to crystallize RNA.
abstract: Preparation of suitably large and well-ordered single crystals is usually the rate-limiting step in the determination of the three-dimensional structure of RNAs and their complexes with proteins by X-ray crystallography. This unit discusses a variety of experimental considerations for obtaining crystals of RNAs and RNA-protein complexes. Topics include design of crystallizable constructs, screening, and optimization of crystallization conditions.
authors.affiliation: Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Ferré-D'Amaré
authors.forename: J A
authors.initials: JA
authors.lastname: Doudna
mesh_terms: D000818:Animals; D001483:Base Sequence; D003460:Crystallization / Q000379:methods*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D009843:Oligoribonucleotides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D012313:RNA / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D013768:Tetrahymena
chemical_list: D009843:Oligoribonucleotides; D011506:Proteins; D012313:RNA