3,4-DGE in peritoneal dialysis fluids cannot be found in plasma after infusion into the peritoneal cavity.
OBJECTIVE
Glucose degradation products (GDPs) are important in the outcome of peritoneal dialysis (PD) treatment. 3,4-dideoxyglucosone-3-ene (3,4-DGE) is the most cytotoxic GDP found in conventionally manufactured fluids and may, in addition, be recruited from 3-deoxyglucosone (3-DG). It is not known what happens with those GDPs in patients during PD. The aim of this study was to investigate if the 3,4-DGE and 3-DG in PD fluids can be found in plasma during treatment.


METHODS
PD patients were dialyzed with a conventional PD fluid containing 43 micromol/L 3,4-DGE and 281 micromol/L 3-DG. Parallel experiments were performed in rats as well as in vitro with human plasma. The rats were dialyzed with a PD fluid containing 100 micromol/L 3,4-DGE and 200 micromol/L 3-DG.


RESULTS
The concentration of 3,4-DGE in the peritoneum decreased at a much higher rate than 3-DG during the dwell. 3,4-DGE was not, however, detected in the plasma of patients or rats during dialysis. The concentration of 3-DG in plasma peaked shortly after infusion of the fluid to the peritoneal cavity. The concentration of 3,4-DGE during experimental incubation in plasma decreased rapidly, while the concentration of 3-DG decreased only 10% as rapidly or less.


CONCLUSIONS
3,4-DGE could not be detected in plasma from either PD patients or rats during dialysis. This is presumably due to its high reactivity. 3-DG may, on the other hand, pass through the membrane and be detected in the blood.
Erixon
Martin
M
Gambro AB, Lund, Sweden. martin.erixon@gambro.com
Wieslander
Anders
A
Lindén
Torbjörn
T
Carlsson
Ola
O
Jönsson
Jan Ake
JA
Simonsen
Ole
O
Kjellstrand
Per
P
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003847:Deoxyglucose / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood; D015314:Dialysis Solutions / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000628:therapy*; D010530:Peritoneal Dialysis; D011753:Pyrones / Q000008:administration & dosage / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
C504711:3,4-dideoxyglucosone-3-ene; D015314:Dialysis Solutions; D011753:Pyrones; D003847:Deoxyglucose; C016350:3-deoxyglucosone