Quantum dot probes for bacteria distinguish Escherichia coli mutants and permit in vivo imaging.
Fluorescent quantum dots coated with zinc(ii)-dipicolylamine coordination complexes can selectively stain a rough Escherichia coli mutant that lacks an O-antigen element and permit optical detection in a living mouse leg infection model.
Leevy
W Matthew
WM
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN 46556, USA.
Lambert
Timothy N
TN
Johnson
James R
JR
Morris
Joshua
J
Smith
Bradley D
BD
D000818:Animals; D001710:Biotin / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry* / Q000235:genetics*; D004927:Escherichia coli Infections / Q000382:microbiology; D005456:Fluorescent Dyes / Q000737:chemistry; D008024:Ligands; D051379:Mice; D008819:Mice, Nude; D008856:Microscopy, Fluorescence; D009154:Mutation; D009942:Organometallic Compounds / Q000737:chemistry; D010847:Picolines / Q000737:chemistry; D045663:Quantum Dots; D019809:Streptavidin / Q000737:chemistry
D005456:Fluorescent Dyes; D008024:Ligands; D009942:Organometallic Compounds; D010847:Picolines; C509592:zinc(II) dipicolylamine; D001710:Biotin; D019809:Streptavidin