title: [Effect of epidermal growth factor on transcription of MCP-1 in rat dental follicle cells].
abstract: OBJECTIVE
  To study the effects of epidermal growth factor (EGF) on the secretion of monocyte chemotactic protein-one (MCP-1) in rat dental follicle cells.
  
  
  METHODS
  Dental follicle was harvested from 4-6 days postnatal Wistar rats and cultured in alpha-MEM with 15% FBS. Cells in the third passage were incubated with EGF at different concentrations to choose the best dosage. MTT assays were used to evaluate cell proliferation. Using the suitable concentration, 10 ng/ml EGF cells were cultured for 0, 0.5, 1, 3 and 6 hours respectively, and then collected for RT-PCR analysis. The results were analyzed with SPSS13.0 software package.
  
  
  RESULTS
  EGF(ng/ml) at 5-10 ng/ml increased the cell proliferation (P<0.05), which reached the peak at 10 ng/ml according to MTT assay (P<0.01). 10 ng/ml and 3 hours EGF exposure up-regulated the cellular MCP-1 expression dramatically (P<0.01).
  
  
  CONCLUSIONS
  Proper concentration of EGF may promote dental follicle cell proliferation, EGF may up-regulate MCP-1 transcription in the rat dental follicle cells.
authors.affiliation: Department of Periodontology, Stomatological Hospital of Shandong University, Jinan, China.
authors.forename: Zong-Xia
authors.initials: ZX
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Lan
authors.initials: L
authors.lastname: Yu
authors.forename: Kai-Liang
authors.initials: KL
authors.lastname: Tang
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Shu
authors.initials: S
authors.lastname: Li
mesh_terms: D000818:Animals; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D003795:Dental Sac / Q000502:physiology*; D066255:EGF Family of Proteins; D004815:Epidermal Growth Factor / Q000502:physiology*; D009930:Organic Chemicals; D051381:Rats; D017208:Rats, Wistar
chemical_list: C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D066255:EGF Family of Proteins; D009930:Organic Chemicals; C420642:alpha minimal essential medium; D004815:Epidermal Growth Factor