Morantel allosterically enhances channel gating of neuronal nicotinic acetylcholine alpha 3 beta 2 receptors.
We studied allosteric potentiation of rat alpha3beta2 neuronal nicotinic acetylcholine receptors (nAChRs) by the anthelmintic compound morantel. Macroscopic currents evoked by acetylcholine (ACh) from nAChRs expressed in Xenopus laevis oocytes increase up to 8-fold in the presence of low concentrations of morantel (< or =10 microM); the magnitude of the potentiation depends on both agonist and modulator concentrations. It is noteworthy that the potentiated currents exceed the maximum currents achieved by saturating (millimolar) concentrations of agonist. Studies of macroscopic currents elicited by prolonged drug applications (100-300 s) indicate that morantel does not increase alpha3beta2 receptor activity by reducing slow (> or =1 s) desensitization. Instead, using outside-out patch-clamp recordings, we demonstrate that morantel increases the frequency of single-channel openings and alters the bursting characteristics of the openings in a manner consistent with enhanced channel gating; these results quantitatively explain the macroscopic current potentiation. Morantel is a very weak agonist alone, but we show that the classic competitive antagonist dihydro-beta-erythroidine inhibits morantel-evoked currents noncompetitively, indicating that morantel does not bind to the canonical ACh binding sites.
Wu
Tse-Yu
TY
Department of Chemistry, Grinnell College, Grinnell, Iowa 50112, USA.
Smith
Caleb M
CM
Sine
Steven M
SM
Levandoski
Mark M
MM
D000494:Allosteric Regulation / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000871:Anthelmintics / Q000494:pharmacology*; D005260:Female; D015640:Ion Channel Gating / Q000187:drug effects* / Q000502:physiology; D009015:Morantel / Q000737:chemistry / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D011978:Receptors, Nicotinic / Q000737:chemistry / Q000502:physiology*; D014982:Xenopus laevis
D000871:Anthelmintics; D011978:Receptors, Nicotinic; C419362:nicotinic receptor alpha3beta2; D009015:Morantel