Gelatin-siloxane hybrid scaffolds with vascular endothelial growth factor induces brain tissue regeneration.
In the brain after infarction or trauma, the tissue becomes pannecrotic and forms a cavity. In such situation, a scaffold is necessary to produce new tissue. In this study, we implanted a new porous gelatin-siloxane hybrid derived from gelatin and 3-(glycidoxypropyl) trimethoxysilane (gelatin-GPTMS) scaffolds into a brain defect, and investigated whether it makes a new brain tissue. In addition, vascular endothelial growth factor (VEGF) was added on gelatin-GPTMS scaffolds and its effect on tissue regeneration was examined. At 30 days after the implantation, the marginal territory of the scaffolds became occupied by newly formed tissue. Immunohistochemical analysis revealed that the new tissue was constituted by endothelial, astroglial and microglial cells, some of which were labeled for bromodeoxyuridine (BrdU). Addition of VEGF promoted numbers of these cells. Thus, combination of gelatin-GPTMS scaffolds and VEGF is preferable for brain regeneration.
Zhang
Hanzhe
H
Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Okayama, 700-8558, Japan.
Kamiya
Tatsushi
T
Hayashi
Takeshi
T
Tsuru
Kanji
K
Deguchi
Kentaro
K
Lukic
Violeta
V
Tsuchiya
Atsushi
A
Yamashita
Toru
T
Hayakawa
Satoshi
S
Ikeda
Yoshio
Y
Osaka
Akiyoshi
A
Abe
Koji
K
D020341:Absorbable Implants; D000818:Animals; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D001973:Bromodeoxyuridine / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004195:Disease Models, Animal; D005780:Gelatin / Q000627:therapeutic use; D008297:Male; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012833:Siloxanes / Q000627:therapeutic use; D054457:Tissue Scaffolds; D042461:Vascular Endothelial Growth Factor A / Q000627:therapeutic use*
D009419:Nerve Tissue Proteins; D012833:Siloxanes; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat; D005780:Gelatin; D001973:Bromodeoxyuridine