Biomarker evidence for mild central nervous system injury after surgically-induced circulation arrest.
Previously, we identified 14-3-3 beta and zeta isoforms and proteolytic fragments of alpha-spectrin as proteins released from degenerating neurons that also rise markedly in cerebrospinal fluid (CSF) following experimental brain injury or ischemia in rodents, but these proteins have not been studied before as potential biomarkers for ischemic central nervous system injury in humans. Here we describe longitudinal analysis of these proteins along with the neuron-enriched hypophosphorylated neurofilament H (pNFH) and the deubiquitinating enzyme UCH-L1 in lumbar CSF samples from 19 surgical cases of aortic aneurysm repair, 7 involving cardiopulmonary bypass with deep hypothermic circulatory arrest (DHCA). CSF levels of the proteins were near the lower limit of detection by Western blot or enzyme-linked fluorescence immunoassay at the onset of surgical procedures, but increased substantially in a subset of cases, typically within 12-24 h. All cases involving DHCA were characterized by >3-fold elevations in CSF levels of the two 14-3-3 isoforms, UCH-L1, and pNFH. Six of 7 also exhibited marked increases in alpha-spectrin fragments generated by calpain, a protease known to trigger necrotic neurodegeneration. Among cases involving aortic cross-clamping but not DHCA, the proteins rose in CSF preferentially in the subset experiencing acute neurological complications. Our results suggest the neuron-enriched 14-3-3beta, 14-3-3zeta, pNFH, UCH-L1, and calpain-cleaved alpha-spectrin may serve as a panel of biomarkers with clinical potential for the detection and management of ischemic central nervous system injury, including for mild damage associated with surgically-induced circulation arrest.
Siman
Robert
R
Department of Neurosurgery, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA. siman@mail.med.upenn.edu
Roberts
Victoria L
VL
McNeil
Elizabeth
E
Dang
Antony
A
Bavaria
Joseph E
JE
Ramchandren
Sindhu
S
McGarvey
Michael
M
D048948:14-3-3 Proteins / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000378:metabolism*; D002315:Cardiopulmonary Bypass / Q000009:adverse effects; D002493:Central Nervous System Diseases / Q000209:etiology* / Q000378:metabolism*; D050792:Circulatory Arrest, Deep Hypothermia Induced / Q000009:adverse effects*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016900:Neurofilament Proteins / Q000134:cerebrospinal fluid; D013049:Spectrin / Q000378:metabolism; D013997:Time Factors; D043222:Ubiquitin Thiolesterase / Q000134:cerebrospinal fluid
D048948:14-3-3 Proteins; D015415:Biomarkers; D016900:Neurofilament Proteins; C482860:UCHL1 protein, human; C482982:YWHAB protein, human; C051774:neurofilament protein H; D013049:Spectrin; D043222:Ubiquitin Thiolesterase