Enhanced production of laccase from Coriolus versicolor NCIM 996 by nutrient optimization using response surface methodology.
Plackett and Burman design criterion and central composite design were applied successfully for enhanced production of laccase by Coriolus versicolor NCIM 996 for the first time. Plackett and Burman design criterion was applied to screen the significance of ten nutrients on laccase production by C. versicolor NCIM 996. Out of the ten nutrients tested, starch, yeast extract, MnSO(4), MgSO(4) x 7H(2)O, and phenol were found to have significant effect on laccase production. A central composite design was applied to determine the optimum concentrations of the significant variables obtained from Plackett-Burman design. The optimized medium composition for production of laccase was (g/l): starch, 30.0; yeast extract, 4.53; MnSO(4), 0.002; MgSO(4) x 7H(2)O, 0.755; and phenol, 0.026, and the optimum laccase production was 6,590.26 (U/l), which was 7.6 times greater than the control.
Arockiasamy
Santhiagu
S
Department of Biotechnology, Kalasalingam University, Krishnankoil, Tamil Nadu, India. asanthiagu@rediffmail.com
Krishnan
Indira Packialakshmi Gurusamy
IP
Anandakrishnan
Nimalanandan
N
Seenivasan
Sabitha
S
Sambath
Agalya
A
Venkatasubramani
Janani Priya
JP
D003470:Culture Media; D007218:Industrial Microbiology / Q000379:methods; D042845:Laccase / Q000096:biosynthesis*; D055454:Trametes / Q000201:enzymology*
D003470:Culture Media; D042845:Laccase