Adhesion of oral streptococci to all-ceramics dental restorative materials in vitro.
In recent years, patients have benefited from the development of better and more esthetic materials, including all-ceramics dental restorative materials. Dental plaque formation on teeth and restorative materials plays an important role in the pathogenesis of oral diseases. This study investigates initial adhesion of stationary phase streptococcal species to different all-ceramics dental restorative materials. The saliva-coated materials were incubated with the bacteria for 1 h in an in vitro flow chamber which mimics environmental conditions in the oral cavity. Number and vitality of adhering bacteria were determined microscopically after staining. Surface roughness and the composition of the materials had no distinctive influence on bacterial adhesion. However, S. mutans and S. sobrinus adhered about tenfold less numerous to all materials than the other streptococcal species. Further, there was a correlation between bacterial vitality and materials' glass content. The results showed that early plaque formation was influenced predominantly by the presence of the salivary pellicle rather than by material dependent parameters whereas the composition of the all-ceramics appeared to have influenced the percentage of viable cells during the adhesion process. This presented in vitro technique may provide a useful model to study the influence of different parameters on adherence of oral streptococcal species.
Meier
R
R
Institute of Preventive Dentistry and Oral Microbiology, Dental School, University of Basel, Hebelstrasse 3, 4056 Basel, Switzerland.
Hauser-Gerspach
I
I
Lüthy
H
H
Meyer
J
J
D001422:Bacterial Adhesion / Q000502:physiology*; D002516:Ceramics; D003741:Dental Deposits / Q000382:microbiology*; D003764:Dental Materials; D044622:Dental Pellicle / Q000382:microbiology; D003793:Dental Restoration, Permanent; D006801:Humans; D008954:Models, Biological; D009055:Mouth / Q000382:microbiology* / Q000601:surgery; D012463:Saliva / Q000382:microbiology; D013295:Streptococcus mutans / Q000502:physiology; D019533:Streptococcus oralis / Q000502:physiology; D013298:Streptococcus sanguis / Q000502:physiology; D016986:Streptococcus sobrinus / Q000502:physiology
D003764:Dental Materials