CYP1A/regucalcin gene expression and edema formation in zebrafish embryos exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin.
In this study, zebrafish eggs were exposed to a relatively low concentration (50 pg/mL) of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) for 72 h and then transferred to vehicle/TCDD-free water for the remainder of the experiments. Mortality, heart rates, edema severity, CYP1A, and regucalcin gene expressions were investigated to study TCDD-induced toxicity in zebrafish during the early life stage. Results indicated that the 50 pg/mL TCDD caused severe and visible developmental toxicity. Further research of the long term and low concentration of TCDD exposure is required.
Wu
Y D
YD
Key Lab of Subtropical Agriculture and Environment, Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, People's Republic of China. W.Y.D_23@hotmail.com
Jiang
L
L
Zhou
Z
Z
Zheng
M H
MH
Zhang
J
J
Liang
Y
Y
D000818:Animals; D002135:Calcium-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000139:chemically induced* / Q000196:embryology / Q000503:physiopathology; D004625:Embryo, Nonmammalian / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D006339:Heart Rate / Q000187:drug effects; D008136:Longevity / Q000187:drug effects; D010490:Pericardial Effusion / Q000139:chemically induced / Q000196:embryology / Q000473:pathology; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism; D013723:Teratogens / Q000633:toxicity*; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
D002135:Calcium-Binding Proteins; D000072317:Polychlorinated Dibenzodioxins; D012333:RNA, Messenger; D013723:Teratogens; D029961:Zebrafish Proteins; D019363:Cytochrome P-450 CYP1A1