Enhanced iodide sequestration by 3-biphenyl-5,6-dihydroimidazo[2,1-b]thiazole in sodium/iodide symporter (NIS)-expressing cells.
The ability of the sodium/iodide symporter (NIS) to take up iodide has long provided the basis for cytoreductive gene therapy and cancer treatment with radioiodide. One of the major limitations of this approach is that radioiodide retention in NIS-expressing cells is not sufficient for their destruction. We identified and characterized a small organic molecule capable of increasing iodide retention in HEK293 cells permanently transfected with human NIS cDNA (hNIS-HEK293) and in the rat thyroid-derived cell line FRTL-5. In the presence of 3-biphenyl-4'-yl-5,6-dihydroimidazo[2,1-b]thiazole (ISA1), the transmembrane iodide concentration gradient was increased up to 4.5-fold. Our experiments indicate that the imidazothiazole derivative acts either by inhibiting anion efflux mechanisms, or by promoting the relocation of iodide into subcellular compartments. This new compound is not only an attractive chemical tool to investigate the mechanisms of iodide flux at the cellular level, but also opens promising perspectives in the treatment of cancer after NIS gene transfer.
Lecat-Guillet
Nathalie
N
Department of Bioorganic Chemistry and Isotopic Labelling, CEA, Institute of Biology and Technology, Gif sur Yvette 91191, France.
Ambroise
Yves
Y
D002460:Cell Line; D015870:Gene Expression; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D007454:Iodides / Q000378:metabolism*; D015394:Molecular Structure; D027981:Symporters / Q000235:genetics / Q000378:metabolism*; D014230:Triazoles / Q000737:chemistry* / Q000494:pharmacology*; D000099209:Sodium-Iodide Symporters
D007093:Imidazoles; D007454:Iodides; D027981:Symporters; D014230:Triazoles; D000099209:Sodium-Iodide Symporters; C029899:imidazole