title: Efficient in vivo gene transfection by stable DNA/PEI complexes coated by hyaluronic acid.
abstract: Plasmid DNA was mixed with polyethyleneimine (PEI) and hyaluronic acid (HA) to afford ternary complexes with negative surface charge regardless of the mixing order. They showed reduced non-specific interactions with blood components. When DNA and PEI were mixed at a high concentration such as that used in in vivo experiments, they soon aggregated, and large particles were formed. On the other hand, pre-addition of HA to DNA prior to PEI effectively diminished the aggregation, and 10% (in volume) of the complexes remained as small particles with a diameter below 80 nm. Those negatively charged small ternary complexes induced a much stronger extra-gene expression in tumor than binary DNA/PEI complex after intratumoral or intravenous injection into the mice bearing B16 cells.
authors.affiliation: Department of Textile Science, Otsuma Women's University Graduate School, Tokyo, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Ito
authors.forename: Naoko
authors.initials: N
authors.lastname: Iida-Tanaka
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Koyama
mesh_terms: D000818:Animals; D004247:DNA / Q000008:administration & dosage* / Q000235:genetics*; D004563:Electrochemistry; D005079:Excipients; D017930:Genes, Reporter; D015316:Genetic Therapy; D049452:Green Fluorescent Proteins / Q000235:genetics; D006820:Hyaluronic Acid / Q000737:chemistry*; D008156:Luciferases / Q000235:genetics; D008297:Male; D008546:Melanoma, Experimental / Q000473:pathology / Q000628:therapy; D051379:Mice; D008856:Microscopy, Fluorescence; D010316:Particle Size; D011094:Polyethyleneimine / Q000737:chemistry*; D014162:Transfection
chemical_list: D005079:Excipients; D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D006820:Hyaluronic Acid; D004247:DNA; D008156:Luciferases