Paradoxical expression of anti-apoptotic and MRP genes on cancer stem-like cell isolated from TJ905 glioblastoma multiforme cell line.
Existence of cancer stem cell is regarded as a main reason for metastasis and/or recurrences. In this study, the cancer stem-like cell derived from TJ905 glioblastoma multiforme cell line was isolated successfully. However, it was observed that generating rate of the cancer stem-like cells was lower than that of TJ905 cells, that expression of the anti-apoptotic and multidrug resistance-associated protein (MRP) genes were paradoxical to the literatures, which showed the uncertainty of cancer stem cells, and that some stem cell was not the solo factor to maintain tumor growth and resist apoptosis and anti-tumor drugs.
Jin
Feng
F
Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Zhao
Lei
L
Zhao
Hong-Yang
HY
Guo
Shou-Gang
SG
Feng
Jun
J
Jiang
Xiao-Bing
XB
Zhang
Shu-Ling
SL
Wei
Yu-Jia
YJ
Fu
Rong
R
Zhao
Jia-Shan
JS
D048868:Adaptor Proteins, Signal Transducing / Q000096:biosynthesis / Q000235:genetics; D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000096:biosynthesis* / Q000235:genetics; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D002417:Cattle; D002454:Cell Differentiation / Q000187:drug effects; D002455:Cell Division; D045744:Cell Line, Tumor / Q000378:metabolism; D002469:Cell Separation; D003470:Culture Media / Q000494:pharmacology; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D005312:Fetal Blood; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis / Q000235:genetics; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000096:biosynthesis / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D014411:Neoplastic Stem Cells / Q000378:metabolism*; D018874:Spheroids, Cellular / Q000166:cytology; D000077022:Survivin
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D003470:Culture Media; D016895:Culture Media, Serum-Free; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D009363:Neoplasm Proteins; D000077022:Survivin; C107489:BIRC5 protein, human; C419866:BIRC7 protein, human; C114462:multidrug resistance-associated protein 3; C421109:multidrug resistance-associated protein 1