title: Infiltration of mast cells in rat colon is a consequence of ischemia/reperfusion.
abstract: Intestinal ischemia as well as mastocytosis occur in patients with inflammatory bowel disease and irritable bowel syndrome. Our aim was to clarify how ischemia with reperfusion (I/R) affects the structure, enteric neurons, and immune cells in the colon. Rats were subjected to colon ischemia for 1 h and reperfused for 1 day up to 20 weeks; sham-operated rats were used as controls. No structural remodeling of the intestinal segment was detected after I/R. The number and distribution of eosinophils were not affected by I/R. Local areas containing numerous mast cells were detected in the muscle layers, the serosa, and in and around the myenteric ganglia 4-20 weeks post ischemia. It was notable that myenteric ganglionic formations within mast-cell-rich areas virtually lacked neurons. Mast cells were rarely found in controls. In conclusion, I/R of the colon attracts mast cells, and death of myenteric neurons occurs in such locations.
authors.affiliation: Neurogastroenterology Unit, Department of Experimental Medical Science, Lund University, BMC B:11, S-22184, Lund, Sweden. elin.sand@med.lu.se
authors.forename: Elin
authors.initials: E
authors.lastname: Sand
authors.forename: Anna
authors.initials: A
authors.lastname: Themner-Persson
authors.forename: Eva
authors.initials: E
authors.lastname: Ekblad
mesh_terms: D000818:Animals; D016923:Cell Death / Q000502:physiology; D002465:Cell Movement / Q000502:physiology*; D003106:Colon / Q000294:innervation / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D017615:Enteric Nervous System / Q000473:pathology; D004804:Eosinophils / Q000473:pathology; D005260:Female; D041981:Gastrointestinal Tract / Q000473:pathology; D008407:Mast Cells / Q000473:pathology*; D009130:Muscle, Smooth / Q000473:pathology; D009474:Neurons / Q000473:pathology; D019001:Nitric Oxide Synthase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D013997:Time Factors; D014660:Vasoactive Intestinal Peptide / Q000378:metabolism
chemical_list: D014660:Vasoactive Intestinal Peptide; D019001:Nitric Oxide Synthase