Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay.
NMO-IgG, a disease-specific autoantibody for neuromyelitis optica, recognizes aquaporin-4 (AQP4) and has been examined by indirect immunofluorescence assay. We developed an enzyme-linked immunosorbent assay (ELISA) to detect anti-AQP4 antibodies by establishing methods for expression in a baculovirus system and purification of recombinant AQP4 as antigen. Elevated anti-AQP4 antibody titers in serum were found in 15 (71%) of 21 patients with neuromyelitis optica, 4.3% of 46 patients with multiple sclerosis, none of 51 normal controls, and 2.6% of 115 patients with other neurological diseases. The ELISA system can be substituted for the conventional NMO-IgG assay.
Hayakawa
Sei
S
Department of Neurology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chou-ku, Chiba 260-8670, Japan.
Mori
Masahiro
M
Okuta
Akiko
A
Kamegawa
Akiko
A
Fujiyoshi
Yoshinori
Y
Yoshiyama
Yasumasa
Y
Mitsuoka
Kaoru
K
Ishibashi
Kenichi
K
Sasaki
Sei
S
Hattori
Takamichi
T
Kuwabara
Satoshi
S
D000328:Adult; D051401:Aquaporin 4 / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology; D009471:Neuromyelitis Optica / Q000097:blood* / Q000276:immunology*
D051401:Aquaporin 4; D001323:Autoantibodies; D007074:Immunoglobulin G