title: Phase optimized liquid chromatography as an instrument for steroid analysis.
abstract: A new method using phase optimized LC (POPLC) for the analysis of steroids is described. The retention factors and the theoretical plate numbers of different steroids were determined for four different stationary phases. Based on these values, an optimal stationary phase composition and the resultant chromatogram have been calculated by an optimization software and compared to the measured data. It is demonstrated that these predicted data show only little differences to the experimental results. Furthermore, it is shown that the overall selectivity of the optimal stationary phase composition is much better than the selectivity of any individual stationary phase.
authors.affiliation: Institute of Organic Chemistry, University of Tuebingen, Tuebingen, Germany.
authors.forename: Maximilian
authors.initials: M
authors.lastname: Kuehnle
authors.forename: Jens
authors.initials: J
authors.lastname: Rehbein
authors.forename: Karsten
authors.initials: K
authors.lastname: Holtin
authors.forename: Benjamin
authors.initials: B
authors.lastname: Dietrich
authors.forename: Manuela
authors.initials: M
authors.lastname: Gradl
authors.forename: Helen
authors.initials: H
authors.lastname: Yeman
authors.forename: Klaus
authors.initials: K
authors.lastname: Albert