Expression of a Canavalia brasiliensis lectin (ConBr) precursor in Pichia pastoris.
A precursor of ConBr, a glucose/mannose-binding plant lectin, was expressed in the yeast Pichia pastoris. Western blot analysis of transformed cells detected an intracellularly recombinant protein band with ca. 34.5 kDa. The recombinant protein was apparently active as suggested by its strong interaction with the mannose-rich yeast cell debris.
Carvalho
C P S
CP
Laboratório de Genética Molecular, Departamento de Biologia, Universidade Federal do Ceará, Fortaleza, Ceará, Brazil.
Rocha
C S
CS
Nepomuceno
D R
DR
Oliveira
J T A
JT
Grangeiro
T B
TB
D032449:Canavalia; D003001:Cloning, Molecular; D015870:Gene Expression; D005822:Genetic Vectors; D004693:Glucan Endo-1,3-beta-D-Glucosidase / Q000378:metabolism; D009097:Multienzyme Complexes / Q000378:metabolism; D009761:Nystatin / Q000378:metabolism / Q000494:pharmacology; D010447:Peptide Hydrolases / Q000378:metabolism; D010843:Pichia / Q000235:genetics* / Q000378:metabolism; D037121:Plant Lectins / Q000096:biosynthesis* / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000096:biosynthesis; D014170:Transformation, Genetic
D009097:Multienzyme Complexes; D037121:Plant Lectins; D011994:Recombinant Proteins; C039268:lectin, Canavalia; C025310:lyticase; D009761:Nystatin; D004693:Glucan Endo-1,3-beta-D-Glucosidase; D010447:Peptide Hydrolases