title: Challenges in translating plasma proteomics from bench to bedside: update from the NHLBI Clinical Proteomics Programs.
abstract: The emerging scientific field of proteomics encompasses the identification, characterization, and quantification of the protein content or proteome of whole cells, tissues, or body fluids. The potential for proteomic technologies to identify and quantify novel proteins in the plasma that can function as biomarkers of the presence or severity of clinical disease states holds great promise for clinical use. However, there are many challenges in translating plasma proteomics from bench to bedside, and relatively few plasma biomarkers have successfully transitioned from proteomic discovery to routine clinical use. Key barriers to this translation include the need for "orthogonal" biomarkers (i.e., uncorrelated with existing markers), the complexity of the proteome in biological samples, the presence of high abundance proteins such as albumin in biological samples that hinder detection of low abundance proteins, false positive associations that occur with analysis of high dimensional datasets, and the limited understanding of the effects of growth, development, and age on the normal plasma proteome. Strategies to overcome these challenges are discussed.
authors.affiliation: Cardiology Division and Center for Immunology and Inflammatory Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Gerszten
authors.forename: Frank
authors.initials: F
authors.lastname: Accurso
authors.forename: Gordon R
authors.initials: GR
authors.lastname: Bernard
authors.forename: Richard M
authors.initials: RM
authors.lastname: Caprioli
authors.forename: Eric W
authors.initials: EW
authors.lastname: Klee
authors.forename: George G
authors.initials: GG
authors.lastname: Klee
authors.forename: Iftikhar
authors.initials: I
authors.lastname: Kullo
authors.forename: Theresa A
authors.initials: TA
authors.lastname: Laguna
authors.forename: Frederick P
authors.initials: FP
authors.lastname: Roth
authors.forename: Marc
authors.initials: M
authors.lastname: Sabatine
authors.forename: Pothur
authors.initials: P
authors.lastname: Srinivas
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Wang
authors.forename: Lorraine B
authors.initials: LB
authors.lastname: Ware
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism*; D016208:Databases, Factual; D006801:Humans; D010949:Plasma / Q000378:metabolism*; D020543:Proteome / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics / Q000639:trends; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D020543:Proteome