title: The genetic contribution to heart rate and heart rate variability in quiescent mice.
abstract: Recent studies have suggested a genetic component to heart rate (HR) and HR variability (HRV). However, a systematic examination of the genetic contribution to the variation in HR and HRV has not been performed. This study investigated the genetic contribution to HR and HRV using a wide range of inbred and recombinant inbred (RI) mouse strains. Electrocardiogram data were recorded from 30 strains of inbred mice and 29 RI strains. Significant differences in mean HR and total power (TP) HRV were identified between inbred strains and RI strains. Multiple significant differences within the strain sets in mean low-frequency (LF) and high-frequency (HF) power were also found. No statistically significant concordance was found between strain distribution patterns for HR and HRV phenotypes. Genomewide interval mapping identified a significant quantitative trait locus (QTL) for HR [LOD (likelihood of the odds) score = 3.763] on chromosome 6 [peak at 53.69 megabases (Mb); designated HR 1 (Hr1)]. Suggestive QTLs for TP were found on chromosomes 2, 4, 5, 6, and 14. A suggestive QTL for LF was found on chromosome 16; for HF, we found one significant QTL on chromosome 5 (LOD score = 3.107) [peak at 53.56 Mb; designated HRV-high-frequency 1 (Hrvhf1)] and three suggestive QTLs on chromosomes 2, 11 and 15. In conclusion, the results demonstrate a strong genetic component in the regulation of resting HR and HRV evidenced by the significant differences between strains. A lack of correlation between HR and HRV phenotypes in some inbred strains suggests that different sets of genes control the phenotypes. Furthermore, QTLs were found that will provide important insight to the genetic regulation of HR and HRV at rest.
authors.affiliation: National Inst. of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. howden@niehs.nih.gov
authors.forename: Reuben
authors.initials: R
authors.lastname: Howden
authors.forename: Eric
authors.initials: E
authors.lastname: Liu
authors.forename: Laura
authors.initials: L
authors.lastname: Miller-DeGraff
authors.forename: Heather L
authors.initials: HL
authors.lastname: Keener
authors.forename: Christopher
authors.initials: C
authors.lastname: Walker
authors.forename: James A
authors.initials: JA
authors.lastname: Clark
authors.forename: Page H
authors.initials: PH
authors.lastname: Myers
authors.forename: D Clay
authors.initials: DC
authors.lastname: Rouse
authors.forename: Tim
authors.initials: T
authors.lastname: Wiltshire
authors.forename: Steven R
authors.initials: SR
authors.lastname: Kleeberger
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D015716:Electrocardiography, Ambulatory; D005838:Genotype; D006339:Heart Rate / Q000235:genetics*; D008126:Lod Score; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D010641:Phenotype; D012123:Pulmonary Ventilation / Q000235:genetics; D040641:Quantitative Trait Loci; D015656:Respiratory Mechanics / Q000235:genetics; D013045:Species Specificity; D013686:Telemetry; D013990:Tidal Volume / Q000235:genetics