[Molecular genetic typing of Legionella pneumophila serogroup 1 isolated in town Verkhnyaya Pyshma using international standards].
Strains of Legionella pneumophila Pyshma-1 and Pyshma-2 were typed according to the international standard - STB protocol developed by EGWLI. Allelic profile of the strains was determined. Identity of strains on the locus pilE, which codes protein important for virulence of bacterium, was shown. Close similarity of nucleotide sequences in Pyshma-1 and Pyshma-2 strains (mainly, 98-100%) was noted. Both strains differed more from reference strain Philadelphia-1 ATCC 33152. Maximal differences (5-6%) were observed in fragment of mompS gene. The study revealed considerable need for conducting of systemic analysis of collected and newly isolated strains in order to get information picture on endemic strains of L. pneumophila in Russia.
Voronina
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Lunin
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D000483:Alleles; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000592:standards*; D019411:Clinical Laboratory Techniques / Q000592:standards*; D005798:Genes, Bacterial; D006801:Humans; D016952:Legionella pneumophila / Q000145:classification* / Q000235:genetics; D007877:Legionnaires' Disease / Q000453:epidemiology / Q000382:microbiology*; D018272:Porins / Q000235:genetics; D012015:Reference Standards; D012426:Russia / Q000453:epidemiology; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D018272:Porins; C072453:major outer membrane protein, Legionella pneumophila