Detection of 1-O-malylglucose: pelargonidin 3-O-glucose-6''-O-malyltransferase activity in carnation (Dianthus caryophyllus).
Carnations have anthocyanins acylated with malate. Although anthocyanin acyltransferases have been reported in several plant species, anthocyanin malyltransferase (AMalT) activity in carnation has not been identified. Here, an acyl donor substance of AMalT, 1-O-beta-D-malylglucose, was extracted and partially purified from the petals of carnation. This was synthesized chemically to analyze AMalT activity in a crude extract from carnation. Changes in the AMalT activity showed close correlation to the accumulation of pelargonidin 3-malylglucoside (Pel 3-malGlc) during the development of red petals of carnation, but neither AMalT activity nor Pel 3-malGlc accumulation was detectable in roots, stems and leaves.
Abe
Yutaka
Y
Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
Tera
Masayuki
M
Sasaki
Nobuhiro
N
Okamura
Masachika
M
Umemoto
Naoyuki
N
Momose
Masaki
M
Kawahara
Nobuo
N
Kamakura
Hiroyuki
H
Goda
Yukihiro
Y
Nagasawa
Kazuo
K
Ozeki
Yoshihiro
Y
D000217:Acyltransferases / Q000378:metabolism*; D000872:Anthocyanins / Q000378:metabolism*; D029749:Dianthus / Q000201:enzymology* / Q000254:growth & development; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008293:Malates / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*
C530861:1-O-malylglucose; D000872:Anthocyanins; D005960:Glucosides; D008293:Malates; D010940:Plant Proteins; C530860:pelargonidin 3-malylglucoside; D000217:Acyltransferases