Analysis of nucleotide sequence of Iranian maize mosaic virus confirms its identity as a distinct nucleorhabdovirus.
The nucleotide sequence of the Iranian maize mosaic rhabdovirus (IMMV) was obtained using a random-PCR method (rPCR) followed by PCR with specific primers. Analysis of the complete nucleotide sequence of the IMMV genes and intergenic regions comprising a total of 12,381 nucleotides (including the partial sequences of leader and trailer regions) revealed six open reading frames (ORF) on the viral complementary RNA (vcRNA). On the basis of its similarities to other rhabdovirus sequences, the IMMV genome consists of 3'-leader-N-P-3-M-G-L-5'-trailer. The intergenic regions contained a characteristic consensus sequence, 3'-AAUUCUUUUUGGGUUU/G-5'. The IMMV gene products showed a high similarity to those of maize mosaic virus and taro vein chlorosis virus and a more distant relationship to other rhabdoviruses. Together with the biological, serological and morphological features described earlier, our molecular data provide evidence that IMMV is a distinct member of the genus Nucleorhabdovirus in the family Rhabdoviridae.
Massah
A
A
Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran.
Izadpanah
K
K
Afsharifar
A R
AR
Winter
S
S
D000595:Amino Acid Sequence; D001483:Base Sequence; D021901:DNA, Intergenic; D005814:Genes, Viral; D016679:Genome, Viral; D007492:Iran; D008969:Molecular Sequence Data; D009029:Mosaic Viruses / Q000235:genetics*; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D010942:Plant Viruses / Q000145:classification* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012209:Rhabdoviridae / Q000145:classification* / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics; D003313:Zea mays / Q000821:virology*
D021901:DNA, Intergenic; D014764:Viral Proteins