Survey of Argentinean human plasma for ochratoxin A.
The presence of ochratoxin A (OTA) in human blood has been reported for many countries, especially in Europe. However, so far no report exists concerning such a presence in Argentina. The aim of this study was to assess OTA concentration in human plasma in two different areas of Buenos Aires province. OTA was determined by high-performance liquid chromatography (HPLC) in 199 plasma samples from blood donors in Mar del Plata and 236 from General Rodriguez. Solid-phase extraction with Bakerbond C-18 cartridge and a final purification with Ochraprep immunoaffinity columns was employed. The limit of quantification of ochratoxin A was 0.019ngml(-1) and the confirmation of OTA was by formation of ochratoxin A methyl ester. The results showed that 63.8% of human plasma samples from Mar del Plata and 62.3% from General Rodriguez were positive for OTA, with Winsorized means of 0.15 and 0.43ngml(-1), respectively. It is important to continue the research to detect the foods responsible of the presence of OTA in plasma.
Pacin
A M
AM
Fundacion de Investigaciones Cientificas Teresa Benedicta de la Cruz, Centro de Investigacion en Micotoxinas, Universidad Nacional de Lujan, Buenos Aires, Argentina. apacin@gmail.com
Ciancio Bovier
E V
EV
Motta
E
E
Resnik
S L
SL
Villa
D
D
Olsen
M
M
D000328:Adult; D000383:Agriculture; D001118:Argentina; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D008297:Male; D009793:Ochratoxins / Q000097:blood*; D013223:Statistics as Topic
D009793:Ochratoxins; C025589:ochratoxin A