title: Molecular determinants of species-specific activation or blockade of TRPA1 channels.
abstract: TRPA1 is an excitatory, nonselective cation channel implicated in somatosensory function, pain, and neurogenic inflammation. Through covalent modification of cysteine and lysine residues, TRPA1 can be activated by electrophilic compounds, including active ingredients of pungent natural products (e.g., allyl isothiocyanate), environmental irritants (e.g., acrolein), and endogenous ligands (4-hydroxynonenal). However, how covalent modification leads to channel opening is not understood. Here, we report that electrophilic, thioaminal-containing compounds [e.g., CMP1 (4-methyl-N-[2,2,2-trichloro-1-(4-nitro-phenylsulfanyl)-ethyl]-benzamide)] covalently modify cysteine residues but produce striking species-specific effects [i.e., activation of rat TRPA1 (rTRPA1) and blockade of human TRPA1 (hTRPA1) activation by reactive and nonreactive agonists]. Through characterizing rTRPA1 and hTRPA1 chimeric channels and point mutations, we identified several residues in the upper portion of the S6 transmembrane domains as critical determinants of the opposite channel gating: Ala-946 and Met-949 of rTRPA1 determine channel activation, whereas equivalent residues of hTRPA1 (Ser-943 and Ile-946) determine channel block. Furthermore, side-chain replacements at these critical residues profoundly affect channel function. Therefore, our findings reveal a molecular basis of species-specific channel gating and provide novel insights into how TRPA1 respond to stimuli.
authors.affiliation: Neuroscience, Global Pharmaceutical Research and Development, Abbott Laboratories, Abbott Park, Illinois 60064-6125, USA. jun.x.chen@abbott.com
authors.forename: Jun
authors.initials: J
authors.lastname: Chen
authors.forename: Xu-Feng
authors.initials: XF
authors.lastname: Zhang
authors.forename: Michael E
authors.initials: ME
authors.lastname: Kort
authors.forename: Jeffrey R
authors.initials: JR
authors.lastname: Huth
authors.forename: Chaohong
authors.initials: C
authors.lastname: Sun
authors.forename: Laura J
authors.initials: LJ
authors.lastname: Miesbauer
authors.forename: Steven C
authors.initials: SC
authors.lastname: Cassar
authors.forename: Torben
authors.initials: T
authors.lastname: Neelands
authors.forename: Victoria E
authors.initials: VE
authors.lastname: Scott
authors.forename: Robert B
authors.initials: RB
authors.lastname: Moreland
authors.forename: Regina M
authors.initials: RM
authors.lastname: Reilly
authors.forename: Philip J
authors.initials: PJ
authors.lastname: Hajduk
authors.forename: Philip R
authors.initials: PR
authors.lastname: Kym
authors.forename: Charles W
authors.initials: CW
authors.lastname: Hutchins
authors.forename: Connie R
authors.initials: CR
authors.lastname: Faltynek
mesh_terms: D000818:Animals; D017487:Ankyrins; D001549:Benzamides / Q000494:pharmacology*; D015220:Calcium Channels / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D006801:Humans; D015640:Ion Channel Gating / Q000187:drug effects* / Q000502:physiology; D009154:Mutation; D009419:Nerve Tissue Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D051381:Rats; D013045:Species Specificity; D000074025:TRPA1 Cation Channel; D050052:TRPC Cation Channels; D050051:Transient Receptor Potential Channels / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: C529446:4-methyl-N-(2,2,2-trichloro-1-(4-nitrophenylsulfanyl)ethyl)benzamide; D017487:Ankyrins; D001549:Benzamides; D015220:Calcium Channels; D009419:Nerve Tissue Proteins; D000074025:TRPA1 Cation Channel; C473350:TRPA1 protein, human; D050052:TRPC Cation Channels; D050051:Transient Receptor Potential Channels; C480976:Trpa1 protein, rat