Association of Gap-43 (neuromodulin) with microtubule-associated protein MAP-2 in neuronal cells.
Gap-43 (B-50, neuromodulin) is a presynaptic protein implicated in axonal growth, neuronal differentiation, plasticity, and regeneration. Its activities are regulated by its dynamic interactions with various neuronal proteins, including actin and brain spectrin. Recently we have shown that Gap-43 co-localizes with an axonal protein DPYSL-3 in primary cortical neurons. In the present study we provide evidence that Gap-43 co-localizes and potentially interacts with microtubule-associated protein MAP-2 in adult and fetal rat brain, as well as in primary neuronal cultures. Our studies suggest that this interaction may be developmentally regulated.
Chakravarthy
Balu
B
National Research Council, Institute for Biological Sciences, 1200 Montreal Road, Ottawa, Ont., Canada. balu.chakravarthy@nrc-cnrc.gc.ca
Rashid
Amal
A
Brown
Leslie
L
Tessier
Luc
L
Kelly
John
J
Ménard
Michel
M
D000595:Amino Acid Sequence; D000818:Animals; D002478:Cells, Cultured; D019922:GAP-43 Protein / Q000032:analysis / Q000378:metabolism*; D047468:Immunoprecipitation; D008869:Microtubule-Associated Proteins / Q000032:analysis / Q000378:metabolism*; D008969:Molecular Sequence Data; D009474:Neurons / Q000737:chemistry / Q000378:metabolism*; D025941:Protein Interaction Mapping; D051381:Rats
D019922:GAP-43 Protein; C489787:MAP2 protein, rat; D008869:Microtubule-Associated Proteins