Definition of MHC supertypes through clustering of MHC peptide-binding repertoires.
Identification of peptides that can bind to major histocompatibility complex (MHC) molecules is important for anticipation of T-cell epitopes and for the design of epitope-based vaccines. Population coverage of epitope vaccines is, however, compromised by the extreme polymorphism of MHC molecules, which is in fact the basis for their differential peptide binding. Therefore, grouping of MHC molecules into supertypes according to peptide-binding specificity is relevant for optimizing the composition of epitope-based vaccines. Despite the fact that the peptide-binding specificity of MHC molecules is linked to their specific amino acid sequences, it is unclear how amino sequence differences correlate with peptide-binding specificities. In this chapter, we detail a method for defining MHC supertypes based on the analysis and subsequent clustering of their peptide-binding repertoires.
Reche
Pedro A
PA
Department of Immunology, Faculated de Medicina, Universidad Complutense de Madrid, Madrid, Spain. parecheg@med.ucm.es
Reinherz
Ellis L
EL
D000483:Alleles; D019295:Computational Biology; D030541:Databases, Genetic; D005787:Gene Frequency; D005838:Genotype; D006680:HLA Antigens / Q000145:classification* / Q000235:genetics* / Q000378:metabolism; D006239:Haplotypes; D006801:Humans; D007125:Immunogenetics; D008285:Major Histocompatibility Complex; D019151:Peptide Library; D010641:Phenotype; D011485:Protein Binding
D006680:HLA Antigens; D019151:Peptide Library