title: Monitoring hygiene on- and at-line is critical for controlling Listeria monocytogenes during produce processing.
abstract: The prevalence of Listeria monocytogenes in different types of produce and on processing plant environments was investigated over a 4-year period in a large produce processing plant in Poland. Prevalence of L. monocytogenes was 46% in frozen vegetables and 41.3% in swab samples taken from the plant environment. Survival studies using artificial inocula demonstrated that the number of Listeria in frozen produce stored for 100 days did not significantly decrease in relation to the initial contamination level. A subset of 129 L. monocytogenes isolates originating from produce and the plant environment were typed by pulsed-field gel electrophoresis. Seventy-six of these isolates were retyped by ribo- and serotyping. Thirteen pulsotypes and 18 ribotypes were distinguished. Persistent Listeria isolates were found even when cleansing and sanitization was applied on a daily basis. Nine (69.2%) of 13 pulsotypes were recovered during a period of more than 2 years. L. monocytogenes of the same pulsotype was isolated from broccoli sampled directly before and after blanching, thus suggesting that blanching at 92 to 95 degrees C for 4 to 8 min did not result in a Listeria-free product, most likely due to massive recontamination. This finding is of importance since blanching is the only critical control point in produce processing. Cross-contamination between the two lines was demonstrated through isolating L. monocytogenes strains indistinguishable by pulsed-field gel electrophoresis from contaminated gloves and floor surfaces.
authors.affiliation: Provincial Sanitary Epidemiology Unit, ul. Kujawska 4, 85-031 Bydgoszcz, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Pappelbaum
authors.forename: Katharina
authors.initials: K
authors.lastname: Grif
authors.forename: Ingrid
authors.initials: I
authors.lastname: Heller
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wüirzner
authors.forename: Ingeborg
authors.initials: I
authors.lastname: Hein
authors.forename: Lueppo
authors.initials: L
authors.lastname: Ellerbroek
authors.forename: Martin
authors.initials: M
authors.lastname: Wagner
mesh_terms: D015373:Bacterial Typing Techniques; D015169:Colony Count, Microbial; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D005511:Food Handling / Q000379:methods*; D005516:Food Microbiology; D005525:Food-Processing Industry / Q000592:standards*; D005838:Genotype; D006801:Humans; D006920:Hygiene; D008089:Listeria monocytogenes / Q000254:growth & development*; D011044:Poland; D015995:Prevalence; D012703:Serotyping; D014675:Vegetables / Q000382:microbiology*