Cobalt supplied per os reduces the mammary Delta9-desaturase index of bovine milk.
Results indicated that the dual marker system of Yb-acetate and Co-EDTA supplied per os reduced the proportion of fatty acids in bovine milk that were products of Delta (9)-desaturase. To verify this effect and identify the responsible marker component, 18 cows (3 cows per treatment) were administered per os a 0.25-L solution of either Co-acetate, Co-EDTA, Co-EDTA + Yb-acetate, EDTA, Yb-acetate, or water twice daily for 5 d. The daily amounts of Co, Yb, and EDTA were, respectively, 3.50, 3.44, and 21.00 g per cow. Milk and blood were sampled and analyzed for content of fatty acids, and blood was sampled and analyzed for Co and cobalamin. Only solutions containing Co had a reducing effect (P < or = 0.01) on fatty acids that were products of Delta(9)-desaturase in milk--cis-9 10:1, cis-9 14:1, cis-9 16:1, cis-9, trans-11 18:2, and cis-9 18:1--with the exception of the solution containing Co-EDTA + Yb-acetate for cis-9 18:1. Of the substrate fatty acids of Delta (9)-desaturase, only 18:0 increased (P < 0.001) in all groups supplied with Co-containing solutions. Thus, Co had a reducing effect (P < or = 0.004) on the Delta (9)-desaturase indices [(product of Delta(9)-desaturase)/(product of Delta(9)-desaturase + substrate of Delta(9)-desaturase)] of milk for cis-9 14:1, cis-9 16:1, cis-9 18:1, and cis-9, trans-11 18:2. There were no differences in Delta(9)-desaturase indices between Co-EDTA and Co-acetate. None of the marker solutions influenced the fatty acid composition of blood plasma, and Co was detected only in the blood samples from cows treated with solutions containing Co. On the basis of these results, we concluded that Co given per os decreased the Delta(9)-desaturase indices of bovine milk.
Taugbøl
O
O
Department of Animal and Aquaculture Sciences, Norwegian University of Life Sciences, 1432 As, Norway. ole.taugbol@umb.no
Karlengen
I J
IJ
Bolstad
T
T
Aastveit
A H
AH
Harstad
O M
OM
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D002417:Cattle; D003035:Cobalt / Q000008:administration & dosage / Q000097:blood / Q000494:pharmacology*; D005260:Female; D008321:Mammary Glands, Animal / Q000187:drug effects* / Q000201:enzymology*; D008892:Milk / Q000737:chemistry / Q000201:enzymology*; D011897:Random Allocation; D013230:Stearoyl-CoA Desaturase / Q000378:metabolism*; D014805:Vitamin B 12 / Q000097:blood
D015415:Biomarkers; D003035:Cobalt; D013230:Stearoyl-CoA Desaturase; D014805:Vitamin B 12