Cryopreservation of mouse adipose tissue-derived stem/progenitor cells.
Adipose tissue-derived stem/progenitor cells (ASCs) have been reported to differentiate not only into mesodermal cells such as osteoblasts, chondorocytes, and adipocytes, but also to endodermal cells such as hepatocytes and insulin-expressing cells. These stem/progenitor cells are expected to be used for variety of regenerative therapies. This study demonstrates the viability and the adipo/osteogenic potential of cryopreserved ASCs using seven cryopreservation solutions, including 10% DMSO, Cell Freezing Medium-DMSO, Cell Freezing Medium-Glycerol, Cell Banker 1, Cell Banker 1+, Cell Banker 2, and CP-1. ASCs were obtained from mouse subcutaneous adipose tissue. The viability of the cryopreserved ASCs was over 90% with Cell Banker 2 preservation, approximately 90% with Cell Banker 1, Cell Banker 1+, or CP-1 preservation, and less than 80% for 10% DMSO, Cell Freezing Medium-DMSO, or Cell Freezing Medium-Glycerol preservation. No difference in the adipo/osteogenic potential was found between cells with or without cryopreservation in Cell Banker 2. These data suggests that Cell Banker 2 is the most effective cryopreservation solution for ASCs and that cryopreserved as well as noncryopreserved ASCs could be applied for regenerative medicine.
Oishi
Koichi
K
Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.
Noguchi
Hirofumi
H
Yukawa
Hiroshi
H
Miyazaki
Takamichi
T
Kato
Ryuji
R
Kitagawa
Yasuo
Y
Ueda
Minoru
M
Hayashi
Shuji
S
D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D002469:Cell Separation; D002470:Cell Survival; D015925:Cryopreservation; D051379:Mice; D019188:Organ Preservation Solutions; D010012:Osteogenesis; D013234:Stem Cells; D023822:Tissue Engineering / Q000379:methods
D019188:Organ Preservation Solutions