Analysis of amadori-glycated phosphatidylethanolamine in the plasma of healthy subjects and diabetic patients by liquid chromatography-tandem mass spectrometry.
Peroxidized phospholipid-mediated cytotoxity, the abnormal increase in the levels of phosphatidylcholine hydroperoxide (PCOOH) found in the plasma of type 2 diabetic patients, is involved in the pathophysiology of many diseases. PCOOH accumulation may be related to Amadori-glycated phosphatidylethanolamine (deoxy-D-fructosyl PE, or Amadori-PE) because Amadori-PE causes oxidative stress. However, the occurrence of lipid glycation products, including Amadori-PE, in vivo remains unclear. We developed a method to analyze Amadori-PE by using quadrupole/linear ion-trap mass spectrometry, the Applied Biosystems 4000 Q TRAP. We found that pyridoxals could easily be condensed with PE before the glucose-PE reaction occurred. The PE-pyridoxal 5'-phosphate adduct was detectable in human red blood cells, and the increased plasma Amadori-PE concentration in streptozotocin-induced diabetic rats was decreased by dietary supplementation with pyridoxal 5'-phosphate. Therefore, it is likely that pyridoxal 5'-phosphate acts as a lipid glycation inhibitor in vivo, and this may contribute to diabetes prevention.
Miyazawa
Teruo
T
Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. miyazawa@biochem.tohoku.ac.jp
Ibusuki
Daigo
D
Yamashita
Shinji
S
Nakagawa
Kiyotaka
K
D002853:Chromatography, Liquid; D003920:Diabetes Mellitus / Q000097:blood*; D017127:Glycation End Products, Advanced / Q000097:blood; D006031:Glycosylation / Q000187:drug effects; D006801:Humans; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000097:blood; D010714:Phosphatidylethanolamines / Q000097:blood* / Q000302:isolation & purification; D011732:Pyridoxal Phosphate / Q000097:blood / Q000494:pharmacology; D012016:Reference Values
D017127:Glycation End Products, Advanced; D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; C057590:phosphatidylcholine hydroperoxide; D011732:Pyridoxal Phosphate