The VLCFA elongase gene family in Arabidopsis thaliana: phylogenetic analysis, 3D modelling and expression profiling.
As precursors of wax compounds, very long chain fatty acids participate in the limitation of non-stomatal water loss and the prevention of pathogen attacks. They also serve as energy storage in seeds and as membrane building blocks. Their biosynthesis is catalyzed by the acyl-CoA elongase, a membrane-bound enzymatic complex containing four distinct enzymes (KCS, KCR, HCD and ECR). Twenty-one 3-ketoacyl-CoA synthase (KCS) genes have been identified in Arabidopsis thaliana genome. In this paper we present an overview of the acyl-CoA elongase genes in Arabidopsis focusing on the entire KCS family. We show that the KCS family is made up of 8 distinct subclasses, according to their phylogeny, duplication history, genomic organization, protein topology and 3D modelling. The analysis of the subcellular localization in tobacco cells of the different subunits of the acyl-CoA elongase shows that all these proteins are localized in the endoplasmic reticulum demonstrating that VLCFA production occurs in this compartment. The expression patterns in Arabidopsis of the acyl-CoA elongase genes suggest several levels of regulations at the tissular or organ level but also under stress conditions suggesting a complex organization of this multigenic family.
Joubès
Jérôme
J
Laboratoire de Biogenèse Membranaire, Université Victor Ségalen Bordeaux 2, CNRS, UMR5200, 146 rue Léo Saignat, Case 92, 33076 Bordeaux Cedex, France. jjoubes@biomemb.u-bordeaux2.fr
Raffaele
Sylvain
S
Bourdenx
Brice
B
Garcia
Christel
C
Laroche-Traineau
Jeanny
J
Moreau
Patrick
P
Domergue
Frédéric
F
Lessire
René
R
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D003066:Coenzyme A Ligases / Q000737:chemistry* / Q000145:classification / Q000235:genetics*; D004721:Endoplasmic Reticulum / Q000201:enzymology; D020869:Gene Expression Profiling; D017343:Genes, Plant; D010802:Phylogeny; D011487:Protein Conformation; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D029681:Arabidopsis Proteins; D003066:Coenzyme A Ligases