title: Analysis of tobamovirus multiplication in Arabidopsis thaliana mutants defective in TOM2A homologues.
abstract: The TOM2A gene of Arabidopsis thaliana encodes a four-pass transmembrane protein that is required for efficient multiplication of a tobamovirus, TMV-Cg. In this study, the involvement of three TOM2A homologues in tobamovirus multiplication in A. thaliana was examined. T-DNA insertion mutations in the three homologues, separately or in combination, did not affect TMV-Cg multiplication, whereas, in the tom2a genetic background, some combinations reduced it. This result suggests that the TOM2A homologues are functional in enhancing TMV-Cg multiplication, but their contribution is much less than TOM2A. Interestingly, the multiplication of another tobamovirus, Tomato mosaic virus, was not drastically affected by any combinations of the mutations in TOM2A and its homologues as far as we examined.
authors.affiliation: Plant-Microbe Interactions Research Unit, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan.
authors.forename: Koki
authors.initials: K
authors.lastname: Fujisaki
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Soko
authors.initials: S
authors.lastname: Kobayashi
authors.affiliation: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Yayoi
authors.initials: Y
authors.lastname: Tsujimoto
authors.affiliation: Graduate School of Life Science, Hokkaido University, Sapporo 060-8589, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Naito
authors.affiliation: Plant-Microbe Interactions Research Unit, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Ishikawa
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry* / Q000821:virology*; D029681:Arabidopsis Proteins / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D054884:Host-Pathogen Interactions; D008565:Membrane Proteins / Q000172:deficiency* / Q000235:genetics; D008969:Molecular Sequence Data; D017354:Point Mutation; D016415:Sequence Alignment; D017898:Tobamovirus / Q000502:physiology*; D014779:Virus Replication
chemical_list: D029681:Arabidopsis Proteins; D002352:Carrier Proteins; D008565:Membrane Proteins; C471269:TOM2 protein, Arabidopsis