title: Functional improvements to beta-lactoglobulin by preparing an edible conjugate with cationic saccharide using microbial transglutaminase [corrected] (MTGase).
abstract: Bovine beta-lactoglobulin (BLG) was conjugated with cationic saccharides to improve its functions. We used a polylysine-dextran conjugate (PL-Dex) as the cationic saccharide which had been prepared by the Maillard reaction. The molar ratio of PL:Dex was 1:1. The emulsifying property of PL in the acidic pH range was improved by conjugating with Dex. BLG and PL-Dex were conjugated by using microbial transglutaminase (MTGase), the effective conjugation being confirmed by SDS-PAGE. The molar ratio of BLG:PL-Dex was 1:1. Structural analyses by a fluorescence study, ELISA with monoclonal antibodies and measurement of the retinol-binding activity indicated that the conjugates had almost retained the native structure of BLG. The emulsifying property of BLG in the acidic pH range and in the presence of NaCl was improved by conjugating with PL-Dex. The immunogenicity of BLG was reduced by this conjugation, while the antigenicity of the BLG-PL-Dex conjugate was similar to that of BLG in BALB/c mice.
authors.affiliation: Division of Agriscience and Bioscience, Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan.
authors.forename: Tomomi
authors.initials: T
authors.lastname: Ikeuchi
authors.forename: Tomomi
authors.initials: T
authors.lastname: Aoki
authors.forename: Tadashi
authors.initials: T
authors.lastname: Yoshida
authors.forename: Koji
authors.initials: K
authors.lastname: Takahashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Hattori
mesh_terms: D000818:Animals; D002417:Cattle; D003911:Dextrans / Q000302:isolation & purification / Q000378:metabolism*; D045703:Emulsifying Agents / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005502:Food; D007782:Lactoglobulins / Q000032:analysis / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D011107:Polylysine / Q000031:analogs & derivatives* / Q000378:metabolism*; D011503:Transglutaminases / Q000378:metabolism*
chemical_list: D003911:Dextrans; D045703:Emulsifying Agents; D007782:Lactoglobulins; D011107:Polylysine; D011503:Transglutaminases