title: CD4+CD25+ regulatory T lymphocytes in tuberculous pleural effusion.
abstract: BACKGROUND
  Active suppression by CD4+CD25+ regulatory T lymphocytes plays an important role in the down-regulation of T cell responses to foreign and self-antigens. This study was conducted to analyze whether the CD4+CD25+ regulatory T cells exist and function normally in tuberculous pleural effusion.
  
  
  METHODS
  The percentages of CD4+CD25+ T cells in pleural effusion and peripheral blood from patients with tuberculous pleurisy and peripheral blood from healthy control subjects were determined by flow cytometry. The expression of forkhead transcription factor Foxp3 was also examined. CD4+CD25+ and CD4+CD25(-) T cells from pleural effusion and blood were isolated, and were cultured to observe the effects of CD4+CD25+ T cells on proliferation response of CD4+CD25(-) T cells in vitro.
  
  
  RESULTS
  There were increased numbers of CD4+CD25+ T cells in tuberculous pleural effusion compared with peripheral blood from both patients with tuberculous pleurisy and normal subjects, and these cells demonstrated a constitutive high-level expression of Foxp3. Moreover, CD4+CD25+ T cells mediated potent inhibition of proliferation response of CD4+CD25(-) T cells.
  
  
  CONCLUSIONS
  The increased CD4+CD25+ T cells in tuberculous pleural effusion express a high level of Foxp3 transcription factor, while potently suppressing the proliferation of CD4+CD25(-) T cells.
authors.affiliation: Institute of Respiratory Diseases, First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi 530021, China.
authors.forename: Xue-jun
authors.initials: XJ
authors.lastname: Qin
authors.forename: Huan-zhong
authors.initials: HZ
authors.lastname: Shi
authors.forename: Qiu-li
authors.initials: QL
authors.lastname: Liang
authors.forename: Lu-ying
authors.initials: LY
authors.lastname: Huang
authors.forename: Hai-bo
authors.initials: HB
authors.lastname: Yang
mesh_terms: D000328:Adult; D005260:Female; D051858:Forkhead Transcription Factors / Q000032:analysis; D006801:Humans; D008213:Lymphocyte Activation; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000209:etiology / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000502:physiology*; D014396:Tuberculosis, Pleural / Q000209:etiology / Q000276:immunology*
chemical_list: C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors