title: Mixed infection, Trichinella spiralis and Trichinella britovi, in a wild boar hunted in the Province of Cáceres (Spain).
abstract: The etiological agents of human trichinellosis are distributed worldwide in domestic and wild animals. In Spain, two morphologically indistinguishable Trichinella species have been described--Trichinella spiralis and Trichinella britovi--that are perpetuated in both domestic and sylvatic cycles. The present work reports a double natural infection involving these species in a wild boar killed by hunters in the Province of Cáceres, Spain. After artificial digestion of the boar's muscles, nine larvae/g were collected. These were characterized by multiplex-PCR and Western-blotting using the Trichinella-specific monoclonal antibodies US5 and US9, and both T. spiralis and T. britovi were detected. The mechanism by which this wild boar came to acquire a mixed infection remains unclear.
authors.affiliation: Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera Majadahonda-Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain.
authors.forename: Esperanza
authors.initials: E
authors.lastname: Rodríguez
authors.forename: Juan
authors.initials: J
authors.lastname: Olmedo
authors.forename: Florencio M
authors.initials: FM
authors.lastname: Ubeira
authors.forename: Carmen
authors.initials: C
authors.lastname: Blanco
authors.forename: Teresa
authors.initials: T
authors.lastname: Gárate
mesh_terms: D000818:Animals; D000835:Animals, Wild; D015153:Blotting, Western / Q000662:veterinary; D018509:DNA, Helminth / Q000032:analysis; D004587:Electrophoresis, Agar Gel / Q000662:veterinary; D007814:Larva / Q000145:classification; D009132:Muscles / Q000469:parasitology; D016133:Polymerase Chain Reaction / Q000662:veterinary; D013030:Spain; D034421:Sus scrofa / Q000469:parasitology*; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology*; D014234:Trichinella / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D017160:Trichinella spiralis / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D014235:Trichinellosis / Q000469:parasitology / Q000662:veterinary*
chemical_list: D018509:DNA, Helminth