[Effects of glucagon like peptide-1 treatment on the alveolar capillary basal lamina in Otsuka Long-Evans Tokushima Fatty rats].
OBJECTIVE
To observe and quantitatively analyze the effect of glucagon like peptide-1 (GLP-1) on the alveolar capillary basal lamina in spontaneous type 2 diabetes mellitus animal model Otsuka Long-Evans Tokushima Fatty (OLETF) rats.


METHODS
Experimental rats were divided into three groups: OLETF group, GLP-1-treated group (OLETF/G group), and Long-Evans Tokushima Otsuka group (LETO group) as control. The ultrastructure and thickness of the alveolar capillary basal lamina in the rats were examined by transmission electron microscopy and morphometry methods.


RESULTS
The fused basal lamina (F-BL) of the alveolar capillary endothelium and type I epithelial basal lamina, and the alveolar capillary endothelium basal lamina (Cap-BL) were thickened in OLETF rats than those of LETO rats [(110.60+/-14.14) nm vs (57.30+/-11.08) nm, and (118.40+/-19.12) nm vs (66.80+/-8.63) nm, P<0.01]. F-BL and Cap-BL were thinned in the OLETF/G group as compared with OLETF group [(79.70+/-5.44) nm vs (110.60+/-14.14) nm and (69.80 +/-3.32) nm vs (118.40+/-19.12) nm, P<0.01].


CONCLUSIONS
Our studies suggest the existence of ultrastructural changes of alveolar capillary basal lamina in OLETF rats. GLP-1 intervention decreases the damage of alveolar capillary basal lamina in OLETF rats.
Tang
Xiu ying
Xy
Peking University First Hospital,Beijing 100034, China. tangxy@bjmu.edu.cn
Wang
Yu
Y
Guo
Xiao hui
Xh
Wang
Shu he
Sh
Chai
Li jun
Lj
Zhang
Ye
Y
D000818:Animals; D001485:Basement Membrane / Q000473:pathology* / Q000648:ultrastructure; D002196:Capillaries / Q000473:pathology*; D003924:Diabetes Mellitus, Type 2 / Q000473:pathology*; D052216:Glucagon-Like Peptide 1 / Q000494:pharmacology*; D008297:Male; D011650:Pulmonary Alveoli / Q000098:blood supply* / Q000473:pathology; D051381:Rats; D020334:Rats, Inbred OLETF
D052216:Glucagon-Like Peptide 1