title: Natural abundance (17)O NMR spectroscopy of rat brain in vivo.
abstract: Oxygen is an abundant element that is present in almost all biologically relevant molecules. NMR observation of oxygen has been relatively limited since the NMR-active isotope, oxygen-17, is only present at a 0.037% natural abundance. Furthermore, as a spin 5/2 nucleus oxygen-17 has a moderately strong quadrupole moment which leads to fairly broad resonances (T(2)=1-4 ms). However, the similarly short T(1) relaxation constants allow substantial signal averaging, whereas the large chemical shift range (>300 ppm) improves the spectral resolution of (17)O NMR. Here it is shown that high-quality, natural abundance (17)O NMR spectra can be obtained from rat brain in vivo at 11.74 T. The chemical shifts and line widths of more than 20 oxygen-containing metabolites are established and the sensitivity and potential for (17)O-enriched NMR studies are estimated.
authors.affiliation: Magnetic Resonance Research Center, Yale University, School of Medicine, New Haven, CT 06520-8043, USA. robin.degraaf@yale.edu
authors.forename: Robin A
authors.initials: RA
authors.lastname: de Graaf
authors.forename: Peter B
authors.initials: PB
authors.lastname: Brown
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Rothman
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Behar
mesh_terms: D000818:Animals; D001923:Brain Chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D010103:Oxygen Isotopes / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity
chemical_list: D010103:Oxygen Isotopes