title: Modulatory effects of 6-carboxymethylthiopurine on activated murine macrophages.
abstract: The immunological activity of macrophages against pathogens in hosts includes the phagocytosis and the production of nitric oxide. We report herein the investigation of the effect of 6-carboxymethylthiopurine on nitric oxide production by murine macrophages as well as its effect on the cell viability and proliferation after stimulus with Mycobacterium bovis bacille Calmette-Guérin, interferon-gamma or a combination of both. J774A.1 macrophages stimulated or not by bacille Calmette-Guérin (20 microg/mL), interferon-gamma or both, were cultured in the presence of 6-carboxymethylthiopurine (125, 250 and 500 microm). Nitric oxide production was measured by the Griess method and cell viability/proliferation by the diphenyltetrazolium assay [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide]. We observed an increase of J774A.1 cell proliferation after stimulus with bacille Calmette-Guérin at 125, 250 and 500 microm (69.1, 124.0 and 89.7%, respectively) and with interferon-gamma at 125 and 250 microm (64.8% and 61.7%, respectively) (p < 0.05). In all cultures treated with 6-carboxymethylthiopurine, interferon-gamma-activated nitric oxide production by J774A.1 cells decreased as well as when subjected to interferon-gamma plus bacille Calmette-Guérin stimuli at 500 microm (p < 0.05). Altogether these data point to an anti-inflammatory effect of 6-carboxymethylthiopurine on stimulated macrophages.
authors.affiliation: Departamento de Parasitologia, Microbiologia e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, Brazil.
authors.forename: Marcio L
authors.initials: ML
authors.lastname: De Paula
authors.forename: Fernanda G
authors.initials: FG
authors.lastname: Braga
authors.forename: Elaine S
authors.initials: ES
authors.lastname: Coimbra
authors.forename: Arturene M L
authors.initials: AM
authors.lastname: Carmo
authors.forename: Henrique C
authors.initials: HC
authors.lastname: Teixeira
authors.forename: Adilson D
authors.initials: AD
authors.lastname: Da Silva
authors.forename: Maria A
authors.initials: MA
authors.lastname: Souza
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D008262:Macrophage Activation; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D015122:Mercaptopurine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051379:Mice; D009569:Nitric Oxide / Q000032:analysis / Q000096:biosynthesis; D011687:Purines / Q000494:pharmacology*
chemical_list: C530174:6-carboxymethylthiopurine; D000893:Anti-Inflammatory Agents; D011687:Purines; D009569:Nitric Oxide; D015122:Mercaptopurine