Evaluation of Permacol as a cultured skin equivalent.
Skin loss following severe burn requires prompt wound closure to avoid such complications as fluid and electrolyte imbalance, infection, immune suppression, and pain. In clinical situations in which insufficient donor skin is available, the development of cultured skin equivalents (dermal matrices seeded with keratinocytes and fibroblasts) may provide a useful alternative. The aim of this study was to assess the suitability of a porcine-derived dermal collagen matrix (Permacol) to function as a cultured skin equivalent in supporting the growth of keratinocytes in vitro and providing cover to full thickness wounds in the BALB C/nude mouse model. A histological comparison was against Glycerol treated-Ethylene Oxide Sterilised Porcine Dermis (Gly-EO Dermis) which has successfully been used as a cultured skin equivalent in previous studies. Both Gly-EO Dermis and to a lesser extent Permacol were able to support the growth of cultured keratinocytes following a 16-day period of cell culture, however, this study was only able to demonstrate the presence of an epidermal layer on Gly-EO dermis 2 weeks after grafting onto full-thickness wounds in the BALB C/nude mouse model.
MacLeod
T M
TM
Restoration of Appearance and Function Trust, Mount Vernon Hospital, Northwood, Middlesex HA62RN, UK. tmhighlander@yahoo.co.uk
Cambrey
A
A
Williams
G
G
Sanders
R
R
Green
C J
CJ
D000818:Animals; D001672:Biocompatible Materials / Q000627:therapeutic use*; D003094:Collagen / Q000627:therapeutic use*; D003451:Cryoprotective Agents / Q000627:therapeutic use; D004202:Disinfectants / Q000627:therapeutic use; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism; D005027:Ethylene Oxide / Q000627:therapeutic use; D005990:Glycerol / Q000627:therapeutic use; D015603:Keratinocytes / Q000378:metabolism / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D023421:Models, Animal; D013552:Swine; D014945:Wound Healing / Q000502:physiology*
D001672:Biocompatible Materials; D003451:Cryoprotective Agents; D004202:Disinfectants; C422414:Permacol; D003094:Collagen; D005027:Ethylene Oxide; D005990:Glycerol