title: Polymorphisms of ADIPOR1 and ADIPOR2 are associated with phenotypes of type 2 diabetes in Koreans.
abstract: OBJECTIVE
  Adiponectin receptors 1 and 2 (ADIPOR1 and ADIPOR2) are considered as candidate genes for type 2 diabetes because they mediate the metabolic effects of adiponectin on target tissues. We investigated whether common polymorphisms of ADIPOR1 and ADIPOR2 are associated with type 2 diabetes or its related phenotypes in Koreans.
  
  
  METHODS
  By sequencing of the DNA samples from 24 unrelated Korean subjects, we selected seven single nucleotide polymorphisms (SNPs) from ADIPOR1 and four SNPs from ADIPOR2 for genotyping in 757 type 2 diabetic patients and 644 nondiabetic subjects.
  
  
  RESULTS
  None of the SNPs were associated with the risk of type 2 diabetes. However, g.-7309A>G (rs75172865) in ADIPOR1 was associated with lower insulin resistance, measured by the homeostasis model assessment of insulin resistance (HOMA-IR). Thereafter, we transfected three different human cell lines with plasmids harbouring g.-7309A>G and found that this variant reduced the promoter activity of ADIPOR1. In ADIPOR2, g.-63442G (novel SNP) and g.33447T (rs1044471) were associated with smaller waist circumference.
  
  
  CONCLUSIONS
  None of the SNPs in either ADIPOR1 or ADIPOR2 were associated with the risk of type 2 diabetes in Koreans. However, the altered activity of the ADIPOR1 promoter was associated with insulin resistance and SNPs of ADIPOR2 were associated with waist circumference.
authors.affiliation: Department of Internal Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Jin Taek
authors.initials: JT
authors.lastname: Kim
authors.forename: Yeonjung
authors.initials: Y
authors.lastname: Kim
authors.forename: Young Min
authors.initials: YM
authors.lastname: Cho
authors.forename: Bo Kyung
authors.initials: BK
authors.lastname: Koo
authors.forename: Eun Kyung
authors.initials: EK
authors.lastname: Lee
authors.forename: Hyoung Doo
authors.initials: HD
authors.lastname: Shin
authors.forename: Hak Chul
authors.initials: HC
authors.lastname: Jang
authors.forename: Jung Won
authors.initials: JW
authors.lastname: Choi
authors.forename: Bermseok
authors.initials: B
authors.lastname: Oh
authors.forename: Kyong Soo
authors.initials: KS
authors.lastname: Park
mesh_terms: D000368:Aged; D044466:Asian People / Q000235:genetics*; D002460:Cell Line; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics*; D005260:Female; D006801:Humans; D007333:Insulin Resistance / Q000235:genetics; D007723:Korea; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D054419:Receptors, Adiponectin / Q000235:genetics*; D012306:Risk; D014162:Transfection; D055105:Waist Circumference
chemical_list: C475418:ADIPOR1 protein, human; C475420:ADIPOR2 protein, human; D054419:Receptors, Adiponectin