title: Dendroflorin retards the senescence of MRC-5 cells.
abstract: The influence of dendroflorin, a potentially active compound extracted from Dendrobium nobile, on cell growth and cell cycles was assessed in a human embryo lung fibroblastic MRC-5 cell line. An MTT assay indicated that dendroflorin benefits cell proliferation. Additionally, we used flow cytometry to examine the cell cycles in senescent MRC-5 cells after treatment with dendroflorin, discovering that dendroflorin could trigger cells in the G1 phase to enter the S phase. Further examination of intracellular ROS contents using DCFH-DA suggested that dendroflorin helps ROS degradation, partly elucidating the mechanism of its effects. All of the above results suggest that dendroflorin is a potential candidate with anti-senescence activity.
authors.affiliation: Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, School of Medicine, Tsinghua University, Beijing, People's Republic of China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Jin
authors.forename: Yu
authors.initials: Y
authors.lastname: Liang
authors.forename: Hong
authors.initials: H
authors.lastname: Xie
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Yao
authors.forename: Zhao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects*; D031667:Dendrobium / Q000737:chemistry; D005434:Flow Cytometry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D016196:S Phase / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles
chemical_list: D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue