Isolation of human neutrophils from venous blood.
Venous blood provides a ready source of large numbers of unstimulated granulocytes and mononuclear cells. Exploiting the differences in the relative densities of the leukocytes circulating in venous blood, one can separate leukocytes from erythrocytes as well as isolate the individual leukocyte populations in high purity for use in ex vivo studies.
Nauseef
William M
WM
Inflammation Program, Department of Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa and Veterans Affairs Medical Center, Coralville, Iowa City, IA, USA.
D001781:Blood Component Removal / Q000379:methods*; D002469:Cell Separation; D002499:Centrifugation, Density Gradient / Q000379:methods; D003911:Dextrans / Q000494:pharmacology; D003973:Diatrizoate / Q000494:pharmacology; D005362:Ficoll / Q000494:pharmacology; D006801:Humans; D008954:Models, Biological; D009504:Neutrophils; D014680:Veins
D003911:Dextrans; D003973:Diatrizoate; D005362:Ficoll