title: Determining design and scale-up parameters for degradation of atrazine with suspended Pseudomonas sp. ADP in aqueous bioreactors.
abstract: This work investigated the kinetic parameters of atrazine mineralization by suspended cells of Pseudomonas sp. ADP in both shake flasks and spherical stirred tank batch reactors (SSTR). The degradation of atrazine and growth of Pseudomonas sp. ADP were studied. Experiments were performed at different temperatures and stirring speeds in both reactors at varying initial concentrations of atrazine. Cell growth and atrazine concentration were monitored over time, and a Monod model with one limiting substrate was used to characterize the kinetic behavior. Temperature, stirring speed, and reactor type were all found to significantly affect the regressed Monod parameters. At 27 degrees C and 200 rpm, for the shaker flask experiments, mu max and Ks were determined to be 0.14 (+/-0.01) h-1 and 1.88 (+/-1.80) mg/L, respectively. At 37 degrees C, mu max and Ks increased to 0.25 (+/-0.05) h-1 and 9.59 (+/-6.55) mg/L, respectively. As expected, stirrer speed was also found to significantly alter the kinetic parameters. At 27 degrees C and 125 rpm, mu max and Ks were 0.04 (+/-0.002) h-1 and 3.72 (+/-1.05) mg/L, respectively, whereas at 37 degrees C and 125 rpm, mu max and Ks were 0.07 (+/-0.008) h-1 and 1.65 (+/-2.06) mg/L. In the SSTR the kinetic parameters mu max and Ks at room temperature were determined to be 0.12 (+/-0.009) h-1 and 2.18 (+/-0.47) mg/L, respectively. Although the mu max values for both types of reactors were similar, the shaker flask experiments resulted in considerable error. Error analysis on calculated values of Ks were found to impact estimates in atrazine concentration by as much as two orders of magnitude, depending on the reactor design, illustrating the importance of these factors in reactor scale-up.
authors.affiliation: The Department of Chemical and Biochemical Engineering and Center for Biocatalysis and Bioprocessing, The University of Iowa, Iowa City, Iowa 52242, USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Biglione
authors.forename: V G J
authors.initials: VG
authors.lastname: Rodgers
authors.forename: Tonya L
authors.initials: TL
authors.lastname: Peeples
mesh_terms: D001280:Atrazine / Q000378:metabolism*; D001673:Biodegradation, Environmental; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D003198:Computer Simulation; D008954:Models, Biological; D011549:Pseudomonas / Q000254:growth & development* / Q000378:metabolism*; D014867:Water
chemical_list: D014867:Water; D001280:Atrazine