Biogenesis of MalF and the MalFGK(2) maltose transport complex in Escherichia coli requires YidC.
The polytopic inner membrane protein MalF is a constituent of the MalFGK(2) maltose transport complex in Escherichia coli. We have studied the biogenesis of MalF using a combination of in vivo and in vitro approaches. MalF is targeted via the SRP pathway to the Sec/YidC insertion site. Despite close proximity of nascent MalF to YidC during insertion, YidC is not required for the insertion of MalF into the membrane. However, YidC is required for the stability of MalF and the formation of the MalFGK(2) maltose transport complex. Our data indicate that YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex.
Wagner
Samuel
S
Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
Pop
Ovidiu Ioan
OI
Pop
Ovidio
O
Haan
Gert-Jan
GJ
Baars
Louise
L
Koningstein
Gregory
G
Klepsch
Mirjam M
MM
Genevaux
Pierre
P
Luirink
Joen
J
de Gier
Jan-Willem
JW
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism / Q000502:physiology*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D008320:Maltose / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D009004:Monosaccharide Transport Proteins / Q000378:metabolism / Q000502:physiology*; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding
D018528:ATP-Binding Cassette Transporters; D029968:Escherichia coli Proteins; C461639:MalF protein, E coli; C079123:MalG protein, E coli; C489398:MalK protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; C412443:YIDC protein, E coli; D008320:Maltose