title: Pressure-assisted cell spinning: a direct protocol for spinning biologically viable cell-bearing fibres and scaffolds.
abstract: We recently pioneered the ability to directly electrospin living cells from which scaffolds to membranes were derived. This protocol, now widely referred to as 'cell electrospinning', is currently undergoing in-depth investigations where the post-treated cell's global gene expression to its sub-cellular components is being investigated for understanding any effects post-treating. Our motivation is to develop this method for the biomedical sciences, in particular for applications in regenerative and therapeutic medicine. In the current work, we unveil a direct cell spinning protocol which is non-electric field driven and which will compete directly with cell electrospinning. We referred to this processing method as 'pressure-assisted spinning' in our previous studies, where we demonstrated this route as an emerging micro/nanotechnology. In the current context, we refer to this processing protocol as 'pressure-assisted cell spinning' (PACS). Our developmental studies on PACS reported here show, for the first time, that this technique could be explored as an alternative approach to cell electrospinning. Pressure-assisted cell spinning now enters the direct biological scaffold to membrane formation league.
authors.affiliation: Department of Mechanical Engineering, University College London, Torrington Place, London WC1E 7JE, UK.
authors.forename: Sumathy
authors.initials: S
authors.lastname: Arumuganathar
authors.forename: Scott
authors.initials: S
authors.lastname: Irvine
authors.forename: Jean R
authors.initials: JR
authors.lastname: McEwan
authors.forename: Suwan N
authors.initials: SN
authors.lastname: Jayasinghe
mesh_terms: D000818:Animals; D002458:Cell Fractionation / Q000295:instrumentation* / Q000379:methods; D002469:Cell Separation / Q000295:instrumentation* / Q000379:methods; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008846:Micromanipulation / Q000295:instrumentation* / Q000379:methods; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000502:physiology*; D011817:Rabbits; D012399:Rotation