title: Evaluation of meningioma aggressiveness by (99m)Tc-Tetrofosmin SPECT.
abstract: OBJECTIVE
  Although meningiomas usually have a benign clinical course, atypical and malignant types of this brain tumor are associated with high recurrence rates and poor outcome; thus, DNA ploidy and S-phase -- as determined by DNA flow cytometry -- are useful indicators of their biological behavior. Brain single-photon emission computed tomography (SPECT) has been suggested as a potentially useful modality for the metabolic assessment of various brain tumors. This study evaluated whether (99m)Tc-Tetrofosmin ((99m)Tc-TF) uptake correlates with meningioma proliferative activity, as assessed by flow cytometry analysis.
  
  
  METHODS
  Ten consecutive patients (3 males, 7 females, mean age 64.6 years) with a diagnosis of a symptomatic intracranial meningioma, planned to undergo surgery, were studied. Brain SPECT by (99m)Tc-TF was performed within a week prior to surgical excision and flow cytometric analysis was performed in the excised tissue. Tumoral radiotracer accumulation was first assessed visually. Semiquantitative image analysis was also performed, by calculating the lesion-to-normal (L/N) uptake ratio.
  
  
  RESULTS
  Benign meningiomas were diagnosed in 8/10 cases, the remaining 2/10 patients had anaplastic lesions. DNA aneuploidy was found in 2 lesions, the remaining tumors were diploid. There was a significant correlation between tracer uptake and the percentage of the cell fraction on S-phase (r=0.733, P=0.05). There was also a positive correlation between tracer uptake and the level of aneuploidy and tumor grade.
  
  
  CONCLUSIONS
  These results imply that (99m)Tc-TF brain SPECT may have the ability to discriminate benign meningiomas from malignant meningiomas pre-operatively, the tracer uptake being a likely indicator of their proliferative activity.
authors.affiliation: Department of Neurosurgery, University Hospital of Ioannina School of Medicine, Ioannina, Greece. alexiougrg@yahoo.gr
authors.forename: George A
authors.initials: GA
authors.lastname: Alexiou
authors.forename: George
authors.initials: G
authors.lastname: Vartholomatos
authors.forename: Spyridon
authors.initials: S
authors.lastname: Tsiouris
authors.forename: Athanasios
authors.initials: A
authors.lastname: Papadopoulos
authors.forename: Athanasios P
authors.initials: AP
authors.lastname: Kyritsis
authors.forename: Konstantinos S
authors.initials: KS
authors.lastname: Polyzoidis
authors.forename: Spyridon
authors.initials: S
authors.lastname: Voulgaris
authors.forename: Andreas D
authors.initials: AD
authors.lastname: Fotopoulos
mesh_terms: D000368:Aged; D002453:Cell Cycle; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D008577:Meningeal Neoplasms / Q000000981:diagnostic imaging*; D008579:Meningioma / Q000000981:diagnostic imaging*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; D015203:Reproducibility of Results; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*; D014057:Tomography, X-Ray Computed
chemical_list: D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; C078700:technetium tc-99m tetrofosmin