Crystallization and preliminary X-ray characterization of full-length Chlamydomonas reinhardtii centrin.
Chlamydomonas reinhardtii centrin is a member of the EF-hand calcium-binding superfamily. It is found in the basal body complex and is important for flagellar motility. Like other members of the EF-hand family, centrin interacts with and modulates the function of other proteins in a calcium-dependent manner. To understand how C. reinhardtii centrin interacts with its protein targets, it has been crystallized in the presence of the model peptide melittin and X-ray diffraction data have been collected to 2.2 A resolution. The crystals are orthorhombic, with unit-cell parameters a = 52.1, b = 114.4, c = 34.8 A, and are likely to belong to space group P2(1)2(1)2.
Alfaro
Elisa
E
Chemistry Department, University of Puerto Rico, Mayagüez, Puerto Rico.
Sosa
Liliana Del Valle
Ldel V
Sanoguet
Zuleika
Z
Pastrana-Ríos
Belinda
B
Schreiter
Eric R
ER
D000818:Animals; D002118:Calcium / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000737:chemistry* / Q000378:metabolism; D016825:Chlamydomonas reinhardtii / Q000378:metabolism*; D003460:Crystallization / Q000379:methods*; D018360:Crystallography, X-Ray; D004926:Escherichia coli; D008555:Melitten / Q000378:metabolism; D013329:Structure-Activity Relationship
D002135:Calcium-Binding Proteins; D008555:Melitten; D002118:Calcium