Recombinant Listeria monocytogenes expressing an immunodominant peptide fails to protect after intravaginal challenge with herpes simplex virus-2.
Recombinant Listeria monocytogenes expressing a type-common herpes simplex virus (HSV) gB-peptide was shown previously to protect against footpad inoculation with HSV-1. We tested this construct for protection against vaginal challenge with HSV-2. Primed mice demonstrated strong recall responses, had modest reductions in HSV-2 DNA in vaginal mucosa, but were not protected from disease.
Muller
William J
WJ
Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, IL, USA. wjmuller@northwestern.edu
Orgun
Nural N
NN
Dong
Lichun
L
Koelle
David M
DM
Huang
Meei-Li
ML
Way
Sing Sing
SS
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D005260:Female; D006558:Herpes Genitalis / Q000276:immunology / Q000517:prevention & control*; D022301:Herpes Simplex Virus Vaccines / Q000008:administration & dosage*; D018258:Herpesvirus 2, Human; D016056:Immunodominant Epitopes / Q000096:biosynthesis* / Q000276:immunology*; D007275:Injections, Intravenous; D008089:Listeria monocytogenes / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D011995:Recombination, Genetic; D018139:Simplexvirus / Q000276:immunology*; D037182:T-Cell Antigen Receptor Specificity; D014611:Vaccination; D014614:Vaccines, Synthetic / Q000008:administration & dosage; D014759:Viral Envelope Proteins / Q000096:biosynthesis* / Q000276:immunology*
D022301:Herpes Simplex Virus Vaccines; D016056:Immunodominant Epitopes; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; C044525:glycoprotein B, herpes simplex virus type 2