title: Impact of contact lens group on antifungal efficacy of multipurpose disinfecting contact lens solutions.
abstract: OBJECTIVE
  To evaluate and compare the in vitro effectiveness of multipurpose contact lens solutions (MPDS) to inhibit fungal colonization of Food and Drug Administration (FDA) contact lens groups.
  
  
  METHODS
  Contact lenses from FDA groups I (low water content, nonionic), II (high water content, nonionic), III (low water content, ionic), and IV (high water content, ionic) were placed in sterile contact lens cases with 2 mL of ReNu MultiPlus, ReNu with MoistureLoc, or OPTI-FREE RepleniSH. Each contact lens-multipurpose solution combination was challenged with 0.1 mL of 10(6) colony-forming units/mL of Fusarium oxysporum and incubated at room temperature. Contact lenses and aliquots from phosphate-buffered saline rinse solutions, multipurpose solutions in cases, and lens cases were sampled at 24, 48, and 72 hours, plated on Sabouraud's agar, incubated at room temperature, and observed for growth (i.e., adhesion) for 1 to 7 days.
  
  
  RESULTS
  Used multipurpose contact lens solutions met or exceeded the 1-log reduction requirement of the FDA stand-alone test. Viable fungal colonies were recovered from group II lenses after 24 hours of storage in all three multipurpose solutions. Colonization was also detected with at least one contact lens-multipurpose solution pairing from groups I, III, and IV. Fungi were not recovered from lens-solution combinations after 24 hours or from phosphate-buffered saline solutions or lens cases at any period.
  
  
  CONCLUSIONS
  Antifungal activity of contaminated multipurpose solutions may be insufficient to prevent fungal colonization of contact lens materials despite meeting or exceeding the FDA stand-alone test criteria. Colonized lens may increase the risk of fungal keratitis.
authors.affiliation: Department of Ophthalmology, Ocular Microbiology Laboratory, Bascom Palmer Eye Institute, Miller School of Medicine, University of Miami, Miami, FL 33136, USA.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Ide
authors.forename: Darlene
authors.initials: D
authors.lastname: Miller
authors.forename: Eduardo C
authors.initials: EC
authors.lastname: Alfonso
authors.forename: Terrence P
authors.initials: TP
authors.lastname: O'Brien
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial; D018639:Contact Lens Solutions / Q000494:pharmacology*; D003263:Contact Lenses, Hydrophilic / Q000382:microbiology*; D005670:Fusarium / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology*
chemical_list: D000935:Antifungal Agents; D018639:Contact Lens Solutions