title: Mucosal vaccination with formalin-inactivated avian metapneumovirus subtype C does not protect turkeys following intranasal challenge.
abstract: Studies were performed to determine if mucosal vaccination with inactivated avian metapneumovirus (aMPV) subtype C protected turkey poults from clinical disease and virus replication following mucosal challenge. Decreases in clinical disease were not observed in vaccinated groups, and the vaccine failed to inhibit virus replication in the tracheas of 96% of vaccinated birds. Histopathologically, enhancement of pulmonary lesions following virus challenge was associated with birds receiving the inactivated aMPV vaccine compared to unvaccinated birds. As determined by an enzyme-linked immunosorbent assay (ELISA), all virus-challenged groups increased serum immunoglobulin (Ig) G and IgA antibody production against the virus following challenge; however, the unvaccinated aMPV-challenged group displayed the highest increases in virus-neutralizing antibody. On the basis of these results it is concluded that intranasal vaccination with inactivated aMPV does not induce protective immunity, reduce virus shedding, or result in decreased histopathologic lesions.
authors.affiliation: Southeast Poultry Research Laboratory, Agricultural Research Service, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA. darrell.kapczynski@seprl.usda.gov
authors.forename: Darrell R
authors.initials: DR
authors.lastname: Kapczynski
authors.forename: Laura L
authors.initials: LL
authors.lastname: Perkins
authors.forename: Holly S
authors.initials: HS
authors.lastname: Sellers
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001980:Bronchi / Q000473:pathology; D005557:Formaldehyde / Q000494:pharmacology; D007070:Immunoglobulin A / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D029121:Metapneumovirus / Q000145:classification / Q000187:drug effects / Q000276:immunology*; D018184:Paramyxoviridae Infections / Q000517:prevention & control / Q000662:veterinary*; D011897:Random Allocation; D020545:Respiratory Mucosa / Q000276:immunology; D013047:Specific Pathogen-Free Organisms; D013997:Time Factors; D014132:Trachea / Q000821:virology; D014422:Turkeys / Q000276:immunology*; D014611:Vaccination / Q000662:veterinary; D015164:Vaccines, Inactivated / Q000008:administration & dosage / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D015164:Vaccines, Inactivated; D014765:Viral Vaccines; D005557:Formaldehyde