Interleukin-3 promotes expansion of hemopoietic-derived CD45+ angiogenic cells and their arterial commitment via STAT5 activation.
Interleukin-3 (IL-3) released by infiltrating inflammatory cells in different pathologic settings contributes to organ and tumor angiogenesis. Here we demonstrate that IL-3 expands a subset of CD45+ circulating angiogenic cells clonally derived from the hemopoietic progenitors. Moreover, CD45+ cells exposed to IL-3 acquire arterial specification and contribute to the formation of vessels in vivo. Depletion of signal transducer and activator of transcription 5 (STAT5) provides evidence that IL-3-mediated cell expansion and arterial morphogenesis rely on STAT5 activation. In addition, by means of Tie2-transgenic mice, we demonstrate that STAT5 also regulates IL-3-induced expansion and arterial specification of bone marrow-derived CD45+ cells. Thus, our data provide the first evidence that, in inflammatory microenvironments containing IL-3, angiogenic cells derived from hemopoietic precursors can act as adult vasculogenic cells. Moreover, the characterization of the signaling pathway regulating these events provides the rationale for therapeutically targeting STAT5 in these pathologic settings.
Zeoli
Annarita
A
Department of Internal Medicine, University of Torino, Torino, Italy.
Dentelli
Patrizia
P
Rosso
Arturo
A
Togliatto
Gabriele
G
Trombetta
Antonella
A
Damiano
Laura
L
di Celle
Paola Francia
PF
Pegoraro
Luigi
L
Altruda
Fiorella
F
Brizzi
Maria Felice
MF
D000818:Animals; D001158:Arteries / Q000254:growth & development*; D001854:Bone Marrow Cells; D049109:Cell Proliferation; D006413:Hematopoietic System / Q000166:cytology*; D006801:Humans; D007377:Interleukin-3 / Q000502:physiology*; D017493:Leukocyte Common Antigens; D051379:Mice; D018919:Neovascularization, Physiologic; D050799:STAT5 Transcription Factor / Q000378:metabolism*; D013234:Stem Cells
D007377:Interleukin-3; D050799:STAT5 Transcription Factor; D017493:Leukocyte Common Antigens