title: [Biocompatibility of combined deproteinized bone coated with hepatocyte growth factor as scaffold for osteoblasts in vitro in fetal rabbits].
abstract: OBJECTIVE
  To determine the cellular compatibility of combined deproteinized bone(DPB) coated with hepatocyte growth factor (HGF), and to observe the adherent effect of osteoblasts in response to HGF.
  
  
  METHODS
  Osteoblasts were isolated from fetal rabbits. Osteoblasts were cultured with DPB coated with HGF and deproteinized bone as experimental group and contral group, respectively. The proliferation and alkalinephosphatase activity were tested. Their growth was examined by inverted phase contrast microscope and scanning electronmicroscope.
  
  
  RESULTS
  The osteoblasts were attached to the outside and inside surfaces and grew well. HGF/DPB could stimulate the alkalinephosphatase activity of the osteoblasts and improve the proliferation of the osteoblasts.
  
  
  CONCLUSIONS
  HGF/DPB has good biocompatibility and bone induction. HGF could improve the adherent effect of DPB on osteoblasts, and it could be used as scaffold material for the bone tissue engineering.
authors.affiliation: Department of Orthopaedics, Taihe Hospital, Medical School of Yunyang, Shiyan Hubei, China. danghsh@tom.com
authors.forename: Hong-sheng
authors.initials: HS
authors.lastname: Dang
authors.forename: Meng
authors.initials: M
authors.lastname: Zhao
authors.forename: Yong-xiang
authors.initials: YX
authors.lastname: Yan
authors.forename: Jia-guo
authors.initials: JG
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D018786:Bone Substitutes / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D011247:Pregnancy; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D017228:Hepatocyte Growth Factor