title: Controlled electrophoretic deposition of multifunctional nanomodules for bioelectrochemical applications.
abstract: The design of an electrochemical glucose sensing device formed by the electrodeposition of multifunctional Au nanoparticles is reported here as a novel concept for an enhanced generic sensing platform. Initially gold nanoparticles (Au) were alternatively coated with a layer of positively charged redox polymer (ORP) and a negatively charged glucose oxidase (GOX) layer alternatively using layer-by-layer methodology to form multifunctional Au/ORP/GOX/ORP particles. The modification and stability of the Au nanoparticles was monitored by using UV-vis spectroscopy and zeta-potential measurements. The modified Au nanoparticles were electrophoretically deposited onto an electrode to produce an electrochemical glucose sensing device. A considerable influence of electrophoretic deposition time and potential was found on the sensing platform response. Preliminary responses to glucose addition showed an enhanced performance by applying an electrophoretic deposition potential of +1.2V vs. Ag/AgCl for 30 min. The observed response in the case of microelectrode geometry was in the range of mAcm(2). This work also shows that the presence of a second outer ORP layer on the functionalised Au nanoparticles improved the response.
authors.affiliation: Bioengineering and Bioelectrochemistry Group, Dept. d'Enginyeria Química, Escola Tècnica Superior d'Enginyeria Química, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Srujan Kumar
authors.initials: SK
authors.lastname: Dondapati
authors.forename: Pablo
authors.initials: P
authors.lastname: Lozano-Sanchez
authors.forename: Ioanis
authors.initials: I
authors.lastname: Katakis
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D003102:Colloids; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D004586:Electrophoresis; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D010084:Oxidation-Reduction
chemical_list: D003102:Colloids; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose