Downregulation of matrix metalloproteinases in hyperplastic dental follicles results in abnormal tooth eruption.
In this study, we compared the gene expression profiles of non-syndromic hyperplastic dental follicle (HDF) fibroblasts and normal dental follicle (NDF) fibroblasts using cDNA microarrays, quantitative PCR, and immunohistochemical staining. Microarray analysis showed that several collagens genes were upregulated in the HDFos, including collagen types I, IV, VIII, and XI and TIMP-1, -3, and -4 (fold ratio > 2.0). In contrast, the expression of MMP-1, -3, -10, and -16 together with IL-8 was more than two fold downregulated. The differential expression of the genes encoding alkaline phosphatase, MMP-1, -3, -8, and IL-8 was confirmed by quantitative RT-PCR, while that of 24 HDFs and 18 NDFs was confirmed by immunohistochemical analysis. However, HDFs showed stronger expression of MMP-3 than NDFs (P < 0.001). Collectively, these results indicate that defective regulation of MMPs mediating connective tissue remodeling may be responsible for abnormal tooth eruption.
Kim
Seong-Gon
SG
Department of Oral and Maxillofacial Surgery, Hallym University, Anyang, Korea. epker@chollian.net
Kim
Myung-Hee
MH
Chae
Chang-Hoon
CH
Jung
Youn-Kwan
YK
Choi
Je-Yong
JY
D002478:Cells, Cultured; D003795:Dental Sac / Q000378:metabolism / Q000473:pathology*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D006965:Hyperplasia / Q000235:genetics; D020782:Matrix Metalloproteinases / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D014079:Tooth Eruption, Ectopic / Q000235:genetics* / Q000378:metabolism
D020782:Matrix Metalloproteinases