Immunosuppressive effect of cyclosporin A on insect humoral immune response.
Cyclosporin A suppressed humoral immune response of Galleria mellonella larvae. Insects were immunized with LPS Pseudomonas aeruginosa and then injected with cyclosporin A. Immunosuppressive effects were expressed both, in larvae treated with cyclosporin A at the initial phase of immune response and at the effector phase of antibacterial immunity. Cyclosporin A moderately decreased lysozyme activity and significantly decreased antibacterial activity peptides against Escherichia coli. Immunosuppressive effects of cyclosporin A were observed after immunoblotting with antibodies anti-G. mellonella lysozyme. Tricine SDS/PAGE shown that synthesis of antibacterial peptides of larvae treated with cyclosporin A was considerably inhibited. Insects of impaired immune response by cyclosporin A action lost protective immunity to insect bacterial pathogen P. aeruginosa.
Fiolka
Marta J
MJ
Department of Invertebrate Immunology, Institute of Biology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland. miwasat@affrc.go.jp
D000818:Animals; D000900:Anti-Bacterial Agents / Q000276:immunology / Q000378:metabolism; D000917:Antibody Formation / Q000187:drug effects* / Q000276:immunology; D016572:Cyclosporine / Q000494:pharmacology*; D006458:Hemolymph / Q000276:immunology / Q000378:metabolism; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D009036:Moths / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D009113:Muramidase / Q000276:immunology; D010572:Pest Control, Biological; D011550:Pseudomonas aeruginosa / Q000276:immunology
D000900:Anti-Bacterial Agents; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D016572:Cyclosporine; D009113:Muramidase