X-ray imaging of various biological samples using a phase-contrast hard X-ray microscope.
In this study, we visualized the internal structures of various bio-samples and found the optimum conditions of test samples for the 7 keV hard X-ray microscope of the Pohang light source. From the captured X-ray images, we could observe the intercellular and intracellular structures of dehydrated human cells and mouse tumor tissues without using any staining materials in a spatial resolution better than 100 nm. The metastasized lung tissue, which was several tens of micrometers in thickness, was found to be very well suited to this hard X-ray microscope system, because it is nearly impossible to observe such a nontransparent and thick sample with a high spatial resolution better than 100 nm using any microscopes such as a soft X-ray microscope, an optical microscope, or an electron microscope.
Kim
Guk Bae
GB
System Bio-Dynamics National Core Research Center, Pohang University of Science and Technology, Republic of Korea.
Yoon
Yae Jin
YJ
Shin
Tae Joo
TJ
Youn
Hwa Shik
HS
Gho
Yong Song
YS
Lee
Sang Joon
SJ
D000818:Animals; D003952:Diagnostic Imaging / Q000379:methods; D006801:Humans; D008168:Lung / Q000166:cytology* / Q000473:pathology; D051379:Mice; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation* / Q000379:methods; D013194:Staining and Labeling / Q000379:methods; D014407:Tumor Cells, Cultured; D014965:X-Rays