Isolation of a novel dehydrin gene from Codonopsis lanceolata and analysis of its response to abiotic stresses.
Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties. The full-length cDNA of ClDhn was 813 bp and contained a 477 bp open reading frame (ORF) encoding a polypeptide of 159 amino acids. Deduced ClDhn protein had high similarities with other plant DHNs. RT-PCR analysis showed that different abiotic stresses such as salt, wounding, chilling and light, triggered a significant induction of ClDhn at different time points within 4-48 hrs post-treatment. This study revealed that ClDhn assisted C. lanceolata in becoming resistant to dehydration.
Pulla
Rama Krishna
RK
Korean Ginseng Center and Ginseng Genetic Resource Bank, Kyung Hee University, Seocheon-dong, Kiheung-gu Yongin, Kyunggi-do, South Korea.
Kim
Yu-Jin
YJ
Kim
Myung Kyum
MK
Senthil
Kalai Selvi
KS
In
Jun-Gyo
JG
Yang
Deok-Chun
DC
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D029745:Codonopsis / Q000235:genetics*; D003080:Cold Temperature; D018076:DNA, Complementary / Q000302:isolation & purification; D003681:Dehydration / Q000235:genetics*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D008969:Molecular Sequence Data; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology
D018076:DNA, Complementary; D010940:Plant Proteins; C070556:dehydrin proteins, plant; D012965:Sodium Chloride; D000040:Abscisic Acid