title: Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions.
abstract: We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.
authors.affiliation: Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, United States of America.
authors.forename: Roberta
authors.initials: R
authors.lastname: Schulte
authors.forename: Jessica
authors.initials: J
authors.lastname: Talamas
authors.forename: Christine
authors.initials: C
authors.lastname: Doucet
authors.forename: Martin W
authors.initials: MW
authors.lastname: Hetzer
mesh_terms: D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D004247:DNA / Q000378:metabolism; D006801:Humans; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D014981:Xenopus
chemical_list: D011506:Proteins; D004247:DNA