title: Cutting edge: MHC class I-Ly49 interaction regulates neuronal function.
abstract: MHC class I molecules (MHC-I) have been implicated in nervous system development in the mouse. In this study we present evidence for the interaction of MHC-I with the NK cell receptor Ly49 in primary cortical neuronal cultures. We show that MHC-I and Ly49 are expressed on neuronal soma and axon surfaces, with Ly49 also present on dendrites. Anti-MHC-I Abs reduce synapsin-I expression and enhance neurite outgrowth and neuronal death. Conversely, anti-Ly49 mAbs increase synapsin-I expression, reduce neurite outgrowth, and promote neuron viability. Because we show that Ly49 genes are selectively expressed in the adult brain, these findings suggest an unsuspected role for the MHC-I-Ly49 interaction in the development and function of the brain.
authors.affiliation: Blanchette Rockefeller Neurosciences Institute, Johns Hopkins University Montgomery County Campus, Rockville, MD 20850, USA. zohar@brni-jhu.org
authors.forename: Ofer
authors.initials: O
authors.lastname: Zohar
authors.forename: Yoram
authors.initials: Y
authors.lastname: Reiter
authors.forename: Jack R
authors.initials: JR
authors.lastname: Bennink
authors.forename: Avital
authors.initials: A
authors.lastname: Lev
authors.forename: Sebastiano
authors.initials: S
authors.lastname: Cavallaro
authors.forename: Sabrina
authors.initials: S
authors.lastname: Paratore
authors.forename: Chaim G
authors.initials: CG
authors.lastname: Pick
authors.forename: Gary
authors.initials: G
authors.lastname: Brooker
authors.forename: Jonathan W
authors.initials: JW
authors.lastname: Yewdell
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism*; D002460:Cell Line; D002540:Cerebral Cortex / Q000254:growth & development* / Q000378:metabolism*; D015870:Gene Expression; D015395:Histocompatibility Antigens Class I / Q000378:metabolism*; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D037181:Lectins, C-Type / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D055630:Receptors, NK Cell Lectin-Like; D015398:Signal Transduction / Q000502:physiology; D013569:Synapses / Q000378:metabolism
chemical_list: D000950:Antigens, Ly; D015395:Histocompatibility Antigens Class I; D037181:Lectins, C-Type; D012333:RNA, Messenger; D055630:Receptors, NK Cell Lectin-Like