title: Synovial VLA-1+ T cells display an oligoclonal and partly distinct repertoire in rheumatoid and psoriatic arthritis.
abstract: VLA-1 integrin expressing T cells are more frequent in inflammatory synovial fluids (SF) compared to peripheral blood. Recent studies suggest that VLA-1 expression mainly marks IFNgamma+ T cells while excluding both IL-4+ and regulatory FoxP3+ T cells. To further characterize the TCR repertoire of the potentially pathogenic VLA-1+ IFNgamma+ T cells, isolated from SF of adult patients with rheumatoid and psoriatic arthritis, we determined the complementarity determining region (CDR)3 spectratypes. Here we show in a cohort of 9 patients that VLA-1+ T cells display a perturbed repertoire that, moreover, differs from that of VLA-1- synovial T cells and even VLA-1+ PB T cells. Importantly, random sequencing of the CDR3 region of the TCR variable beta (BV) 6.1 gene of both VLA-1+ and VLA-1- synovial T cells, in one patient, revealed that their sequences were by and large different (29 out of 33 clones). Thus, our results imply that VLA-1+ T cells that infiltrate into inflamed joints represent a partly distinct and highly oligoclonal population of Th1 cells, probably selected by unique antigens.
authors.affiliation: Cancer Research Center and Institute of Hematology, The Chaim Sheba Medical Center, Tel Aviv University, Israel. Itamar.Goldstein@sheba.health.gov.il
authors.forename: Itamar
authors.initials: I
authors.lastname: Goldstein
authors.forename: Amos J
authors.initials: AJ
authors.lastname: Simon
authors.forename: Shomron
authors.initials: S
authors.lastname: Ben Horin
authors.forename: Sarit
authors.initials: S
authors.lastname: Matzri
authors.forename: Alexander
authors.initials: A
authors.lastname: Koltakov
authors.forename: Pnina
authors.initials: P
authors.lastname: Langevitz
authors.forename: Gideon
authors.initials: G
authors.lastname: Rechavi
authors.forename: Ninette
authors.initials: N
authors.lastname: Amariglio
authors.forename: Ilan
authors.initials: I
authors.lastname: Bank
mesh_terms: D015535:Arthritis, Psoriatic / Q000276:immunology*; D001172:Arthritis, Rheumatoid / Q000276:immunology*; D022801:Complementarity Determining Regions / Q000235:genetics; D005434:Flow Cytometry; D006801:Humans; D039222:Integrin alpha1beta1 / Q000378:metabolism*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics; D013582:Synovial Fluid / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: D022801:Complementarity Determining Regions; D039222:Integrin alpha1beta1; D016693:Receptors, Antigen, T-Cell, alpha-beta