title: Human mesenchymal stem cells as a stable source of VEGF-producing cells.
abstract: Vascular endothelial growth factor (VEGF) is a positive regulator and plays a crucial role in angiogenesis. We demonstrate that VEGF was highly expressed in cultures of human bone marrow-derived mesenchymal stem cells (hMSCs) and the high expression level was maintained during prolonged culture periods (checked up to passage 10). We also confirmed that in vivo hMSCs engrafted into immunodeficient mice could survive and secreted human VEGF. These findings suggest that implantation of hMSCs is a practical means as a source of VEGF production and might be effective in neoangiogenesis.
authors.affiliation: Tissue Engineering Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, 3-11-46 Nakoji, Amagasaki, Hyogo, Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Kagiwada
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Yashiki
authors.forename: Akira
authors.initials: A
authors.lastname: Ohshima
authors.forename: Mika
authors.initials: M
authors.lastname: Tadokoro
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Nagaya
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D016207:Cytokines / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D016513:Mice, SCID; D018919:Neovascularization, Physiologic; D040081:Protein Array Analysis; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis*
chemical_list: D016207:Cytokines; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A