title: The mammalian CHORD-containing protein melusin is a stress response protein interacting with Hsp90 and Sgt1.
abstract: Melusin is a mammalian muscle specific CHORD containing protein capable of activating signal transduction pathways leading to cardiomyocytes hypertrophy in response to mechanical stress. To define melusin function we searched for molecular partners possibly involved in melusin dependent signal transduction. Here we show that melusin and heat shock proteins are co-regulated. Moreover, melusin directly binds to Hsp90, a ubiquitous chaperone involved in regulating several signaling pathways. In addition, melusin interacts with Sgt1, an Hsp90 binding molecule. Melusin does not behave as an Hsp90 substrate but rather as a chaperone capable to protect citrate synthase from heat induced aggregation. These results describe melusin as a new component of the Hsp90 chaperone machinery.
authors.affiliation: Department of Genetics, Biology and Biochemistry, University of Torino, Molecular Biotechnology Center, via Nizza, 52, Torino, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Sbroggiò
authors.forename: Roberta
authors.initials: R
authors.lastname: Ferretti
authors.forename: Elena
authors.initials: E
authors.lastname: Percivalle
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Gutkowska
authors.forename: Alicja
authors.initials: A
authors.lastname: Zylicz
authors.forename: Wojciech
authors.initials: W
authors.lastname: Michowski
authors.forename: Jacek
authors.initials: J
authors.lastname: Kuznicki
authors.forename: Federica
authors.initials: F
authors.lastname: Accornero
authors.forename: Beniamina
authors.initials: B
authors.lastname: Pacchioni
authors.forename: Gerolamo
authors.initials: G
authors.lastname: Lanfranchi
authors.forename: Jorg
authors.initials: J
authors.lastname: Hamm
authors.forename: Emilia
authors.initials: E
authors.lastname: Turco
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Silengo
authors.forename: Guido
authors.initials: G
authors.lastname: Tarone
authors.forename: Mara
authors.initials: M
authors.lastname: Brancaccio
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D003598:Cytoskeletal Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism*; D047468:Immunoprecipitation; D051379:Mice; D018832:Molecular Chaperones / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D018841:HSP90 Heat-Shock Proteins; C122158:Itgb1bp2 protein, mouse; D018832:Molecular Chaperones; D009124:Muscle Proteins; C530258:Sugt1 protein, mouse