[Molecular and epidemiological characteristics of tick-borne encephalitis virus in the Sverdlovsk Region on the basis of genotype-specific RT-PCR].
Studies and complete awareness of the regional and epidemiological properties of tick-borne encephalitis virus (TBEV) allow one to improve methods for preventing, diagnosing, and treating its severe neurological infection. The authors have developed reverse-transcriptase polymerase chain reaction (RT-PCR) systems for the detection of RNA of TBEV and for the determination of its genotype in the ticks and clinical materials. RT-PTC was shown to have a higher sensitivity and specificity than the practically used enzyme immunoassay system. Despite significant variations in the spread of infected ticks in some districts of the Sverdlovsk Region (5-12%), the average regional value was 8% over the study period. The authors have studied more than a thousand of ticks collected from the nature and humans in the epidemic season of 2005-2006. There was a virtually complete predominance (more than 95%) of the Ural-Siberian genotype, with rare cases of the European genotype (slightly more than 4%) being detected. The Far-Eastern genotype was not detected.
Kovalev
S Iu
SIu
Umpeleva
T V
TV
Snitkovskaia
T E
TE
Kiliatsina
A S
AS
Romanenko
V V
VV
Kokorev
V S
VS
Glinskikh
N P
NP
D000818:Animals; D001096:Arachnid Vectors / Q000821:virology; D001733:Bites and Stings; D004669:Encephalitis Viruses, Tick-Borne / Q000145:classification* / Q000235:genetics; D004675:Encephalitis, Tick-Borne / Q000175:diagnosis / Q000517:prevention & control / Q000821:virology*; D004784:Environmental Monitoring; D062665:Epidemiological Monitoring; D006801:Humans; D018884:Ixodes / Q000821:virology; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D012426:Russia / Q000453:epidemiology; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins