title: Mast cells regulate the magnitude and the cytokine microenvironment of the contact hypersensitivity response.
abstract: The role that mast cells play during contact hypersensitivity (CS) response is unclear because some studies have shown that mast cell-deficient mice have relatively intact CS responses whereas others have shown opposing results. Mast cells secrete a wide range of immunomodulatory mediators and can potentially influence the type of immune response generated in the skin during CS. Therefore, we examined the type of microenvironment generated during CS in both W/Wv mast cell-deficient and wild-type mice in response to different immunizing doses of hapten (oxazolone). The CS response elicited after low-dose oxazolone was significantly diminished in W/Wv mice compared with wild-type mice. Unexpectedly, the CS response elicited in W/Wv mice immunized with high-dose oxazolone was more severe compared with wild-type mice. In addition, after immunization with high-dose oxazolone, the granulocyte infiltrate in W/Wv mice was increased by twofold compared with wild-type mice. A shift in the cytokine milieu toward the expression of type-1 cytokines as well as a significant increase in the local adhesion of neutrophils and CD4 T cells in the microvasculature of the skin was observed after hapten challenge in W/Wv mice immunized with high-dose oxazolone compared with wild-type mice. These results suggest that mast cells can act as regulators and inducers of the inflammatory response depending on immunizing stimulus strength.
authors.affiliation: Immunology Research Group, University of Calgary, Calgary, AB, T2N 4N1, Canada.
authors.forename: M Ursula
authors.initials: MU
authors.lastname: Norman
authors.forename: John
authors.initials: J
authors.lastname: Hwang
authors.forename: Sara
authors.initials: S
authors.lastname: Hulliger
authors.forename: Claudine S
authors.initials: CS
authors.lastname: Bonder
authors.forename: Jun
authors.initials: J
authors.lastname: Yamanouchi
authors.forename: Pere
authors.initials: P
authors.lastname: Santamaria
authors.forename: Paul
authors.initials: P
authors.lastname: Kubes
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002448:Cell Adhesion / Q000276:immunology; D002478:Cells, Cultured; D016207:Cytokines / Q000276:immunology*; D003877:Dermatitis, Contact / Q000276:immunology* / Q000503:physiopathology; D006241:Haptens; D008407:Mast Cells / Q000276:immunology* / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D008833:Microcirculation / Q000276:immunology; D010081:Oxazolone / Q000276:immunology / Q000494:pharmacology; D012867:Skin / Q000098:blood supply / Q000276:immunology*
chemical_list: D016207:Cytokines; D006241:Haptens; D010081:Oxazolone