title: Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples.
abstract: The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.
authors.affiliation: Department of Microbiology, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong, SAR, People's Republic of China.
authors.forename: Kwok Hung
authors.initials: KH
authors.lastname: Chan
authors.forename: Wing Cheong
authors.initials: WC
authors.lastname: Yam
authors.forename: Chiu Mei
authors.initials: CM
authors.lastname: Pang
authors.forename: Kit Man
authors.initials: KM
authors.lastname: Chan
authors.forename: Siu Yan
authors.initials: SY
authors.lastname: Lam
authors.forename: Kam Fai
authors.initials: KF
authors.lastname: Lo
authors.forename: Leo L M
authors.initials: LL
authors.lastname: Poon
authors.forename: J S Malik
authors.initials: JS
authors.lastname: Peiris
mesh_terms: D001331:Automation; D004267:DNA Viruses / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D006723:Hong Kong; D006761:Hospitals; D006801:Humans; D009305:Nasopharynx / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012328:RNA Viruses / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis*
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral