Macrophage physiological function after superparamagnetic iron oxide labeling.
Our goal was to analyze the changes in morphology and physiological function (phagocytosis, migratory capabilities, humoral and cellular response, and nitric oxide secretion) of murine macrophages after labeling with a clinically used superparamagnetic iron oxide (SPIO), ferucarbotran. In SPIO-treated macrophages, nanoparticles were taken up in the cytoplasm and accumulated in a membrane-bound organelle. Macrophage proliferation and viability were not modified after SPIO labeling. Phagocytic function decreased after labeling with only 10 microg Fe/mL SPIO, whereas other functions including migration and production of tumor necrosis factor-alpha and nitric oxide increased at the highest SPIO concentration (100 microg Fe/mL).
Hsiao
Jong-Kai
JK
Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
Chu
Hung-Hao
HH
Wang
Yu-Hsiu
YH
Lai
Chih-Wei
CW
Chou
Pi-Tai
PT
Hsieh
Sung-Tsang
ST
Wang
Jaw-Lin
JL
Liu
Hon-Man
HM
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D048429:Cell Size; D002470:Cell Survival; D003287:Contrast Media; D005290:Ferric Compounds; D008264:Macrophages / Q000166:cytology* / Q000502:physiology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D051379:Mice; D010587:Phagocytosis / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods
D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide