Quantitative immunohistochemical analysis of nitric oxide synthases and apoptosis regulator proteins in the fetal rat brain following maternal uterine artery ligation.
The purpose of this study was to determine the relation between nitric oxide synthases (calcium-independent iNOS and calcium-dependent eNOS) and apoptosis regulator proteins (anti-apoptotic Bcl-2, pro-apoptotic p53) of fetal rat brain in experimental intrauterine growth retardation (IUGR) model via quantitative immunohistochemistry. Cortical zone of parietal cerebral cortex and ventricular zone of third ventricle were studied following bilateral uterine artery ligation on gestational day 18. Significant increase in iNOS immunoreactivity was determined in parietal cerebral cortex and ventricular zones as eNOS immunoreactivity increased in ventricular zone of IUGR group. Bcl-2 expression was significantly decreased in ventricular zone; whereas cortical zone of IUGR group expressed p53 immunoreactivity.
Uysal
Aysegul
A
Department of Histology and Embryology, Ege University, Faculty of Medicine, Izmir, Turkey. aysegul.uysal@ege.edu.tr
Oktem
Gulperi
G
Yilmaz
Ozlem
O
Uslu
Serap
S
Aktug
Huseyin
H
Yurtseven
Mine Ertem
ME
D000818:Animals; D051017:Apoptosis Regulatory Proteins / Q000032:analysis* / Q000096:biosynthesis; D001158:Arteries / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D001923:Brain Chemistry / Q000502:physiology; D005260:Female; D005317:Fetal Growth Retardation / Q000201:enzymology / Q000473:pathology; D005333:Fetus / Q000737:chemistry / Q000201:enzymology / Q000473:pathology; D007150:Immunohistochemistry; D008026:Ligation; D052247:Nitric Oxide Synthase Type II / Q000032:analysis* / Q000096:biosynthesis; D052250:Nitric Oxide Synthase Type III / Q000032:analysis* / Q000096:biosynthesis; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D014599:Uterus / Q000098:blood supply* / Q000737:chemistry* / Q000201:enzymology
D051017:Apoptosis Regulatory Proteins; D052247:Nitric Oxide Synthase Type II; D052250:Nitric Oxide Synthase Type III