title: Agrobacterium-mediated transformation of large-flowered purslane (Portulaca grandiflora H.).
abstract: Transformation of Portulaca grandiflora has been developed with Agrobacterium tumefaciens strains A281 and T1272. Transformation was assessed by the following criteria: selection of hormone independent callus, antibiotic-resistant callus, and transgenic antibiotic-resistant plants. In addition, DNA hybridization analysis demonstrated that the DNA from tumor lines contained sequences homologous to binary vector T-DNA of strain A281. Following transformation with strain T1272, segregation analysis of the progeny of transgenic plants showed that the transgene was inherited in a Mendelian manner. The kanamycin-resistant progeny tested contained the T-DNA sequence of the strain T1272.
authors.forename: B D
authors.initials: BD
authors.lastname: Rossi-Hassani
authors.forename: F
authors.initials: F
authors.lastname: Bennani
authors.forename: J P
authors.initials: JP
authors.lastname: Zryd