Live single-cell molecular analysis by video-mass spectrometry.
The direct molecular analysis of a live single cell viewed under a video-microscope has been developed. The cell contents are sucked into a nano-electrospray tip, and hundreds of peaks of ionic compounds of low molecular weight are detected by nano-ESI Q-TOF mass spectrometry (MS). Cell-specific MS peaks in a single mouse-embryonic fibroblasts cell are extracted by a t-test, and one of the peaks is proceeded to MS/MS analysis for molecular identification. This method is direct and quick to identify the molecules of a cell with simultaneous observation by a video-microscope.
Tsuyama
Naohiro
N
Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Mizuno
Hajime
H
Tokunaga
Emi
E
Masujima
Tsutomu
T
D000818:Animals; D002470:Cell Survival; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D015394:Molecular Structure; D011392:Proline / Q000737:chemistry; D041701:Swiss 3T3 Cells; D014743:Videotape Recording / Q000379:methods*
D011392:Proline