title: Anatomical element localization by EDXS in Grevillea exul var. exul under nickel stress.
abstract: Grevillea exul var. exul, an endemic serpentinic Proteaceae of New Caledonia, was chosen to study the spatial distribution of Ni because this species supports strong content of metals, which can allow important absorptions thus detectable by microanalysis. Fine transversal sections of axenic G. exul var. exul plants grown during 15 days on nickel sulphate medium were examined by EDXS microanalysis. It showed that in Ni treated plants, Ni was concentrated mostly in the phloem compared to the xylem and the epidermis, either in roots or in the basal part of the stems and was mostly in the epidermis in the upper part of the stems and not detectable in the leaves. This metal took the place of P and K in the treated plants whereas the localization of these macroelements was quite uniform in control sections. We assume that a mechanism of phloem loading is implicated to restrict Ni accumulation in G. exul var. exul.
authors.affiliation: Equipe Biomarqueurs et Bioindicateurs Environnementaux, Institut Méditerranéen d'Ecologie et de Paléoécologie, UMR CNRS 6116, Université de Provence, Case 17, 3 Place Victor Hugo, 13331 Marseille Cedex 3, France. Jacques.Rabier@univ-provence.fr
authors.forename: J
authors.initials: J
authors.lastname: Rabier
authors.forename: I
authors.initials: I
authors.lastname: Laffont-Schwob
authors.forename: R
authors.initials: R
authors.lastname: Notonier
authors.forename: B
authors.initials: B
authors.lastname: Fogliani
authors.forename: S
authors.initials: S
authors.lastname: Bouraïma-Madjèbi
mesh_terms: D001673:Biodegradation, Environmental; D002713:Chlorine / Q000032:analysis; D007090:Image Interpretation, Computer-Assisted; D008854:Microscopy, Electron; D009532:Nickel / Q000032:analysis / Q000633:toxicity*; D052585:Phloem / Q000737:chemistry; D010758:Phosphorus / Q000032:analysis; D019441:Plant Epidermis / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011188:Potassium / Q000032:analysis; D039105:Proteaceae / Q000737:chemistry*; D012964:Sodium / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis / Q000633:toxicity*; D013052:Spectrometry, X-Ray Emission; D052584:Xylem / Q000737:chemistry
chemical_list: D012989:Soil Pollutants; D010758:Phosphorus; C029938:nickel sulfate; D002713:Chlorine; D009532:Nickel; D012964:Sodium; D011188:Potassium