title: Genetic characterization of H1N1, H1N2 and H3N2 swine influenza virus in Thailand.
abstract: Swine have been known to be a suitable host for influenza A virus. In Thailand, phylogenetic analysis on swine influenza virus (SIV) has as yet not been attempted. The present report presents molecular and phylogenetic analysis performed on SIV in Thailand. In this study, 12 SIV isolates from the central and eastern part of Thailand were subtyped and the molecular genetics of hemagglutinin and neuraminidase were elucidated. Three subtypes, H1N1, H1N2 and H3N2, are described. Phylogenetic analysis of the SIV hemagglutinin and neuraminidase genes shows individual clusters with swine, human or avian influenza virus at various global locations. Furthermore, amino acid substitutions were detected either at the receptor binding site or the antigenic sites of the hemagglutinin gene.
authors.affiliation: Center of Excellence in Clinical Virology, Department of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
authors.forename: Salin
authors.initials: S
authors.lastname: Chutinimitkul
authors.forename: Nattakarn
authors.initials: N
authors.lastname: Thippamom
authors.forename: Sudarat
authors.initials: S
authors.lastname: Damrongwatanapokin
authors.forename: Sunchai
authors.initials: S
authors.lastname: Payungporn
authors.forename: Roongroje
authors.initials: R
authors.lastname: Thanawongnuwech
authors.forename: Alongkorn
authors.initials: A
authors.lastname: Amonsin
authors.forename: Pitirat
authors.initials: P
authors.lastname: Boonsuk
authors.forename: Donruethai
authors.initials: D
authors.lastname: Sreta
authors.forename: Napawan
authors.initials: N
authors.lastname: Bunpong
authors.forename: Rachod
authors.initials: R
authors.lastname: Tantilertcharoen
authors.forename: Pornchai
authors.initials: P
authors.lastname: Chamnanpood
authors.forename: Sujira
authors.initials: S
authors.lastname: Parchariyanon
authors.forename: Apiradee
authors.initials: A
authors.lastname: Theamboonlers
authors.forename: Yong
authors.initials: Y
authors.lastname: Poovorawan
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000818:Animals; D005814:Genes, Viral / Q000235:genetics; D006389:Hemagglutinins, Viral / Q000235:genetics; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics; D009980:Influenza A virus / Q000145:classification / Q000235:genetics*; D008969:Molecular Sequence Data; D009439:Neuraminidase / Q000235:genetics; D009976:Orthomyxoviridae Infections / Q000821:virology*; D010802:Phylogeny; D016415:Sequence Alignment; D013552:Swine / Q000821:virology; D013785:Thailand; D014764:Viral Proteins / Q000235:genetics
chemical_list: D006389:Hemagglutinins, Viral; D014764:Viral Proteins; D009439:Neuraminidase