title: One-way biohydrogen transfer for oxidation of sec-alcohols.
abstract: Quasi-irreversible oxidation of sec-alcohols was achieved via biocatalytic hydrogen transfer reactions using alcohol dehydrogenases employing selected ketones as hydrogen acceptors, which can only be reduced but not oxidized. Thus, only 1 equiv of oxidant was required instead of a large excess. For the oxidation of both isomers of methylcarbinols a single nonstereoselective short-chain dehydrogenase/reductase from Sphingobium yanoikuyae was identified and overexpressed in E. coli.
authors.affiliation: Research Centre Applied Biocatalysis c/o Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Graz, Austria.
authors.forename: Iván
authors.initials: I
authors.lastname: Lavandera
authors.forename: Alexander
authors.initials: A
authors.lastname: Kern
authors.forename: Verena
authors.initials: V
authors.lastname: Resch
authors.forename: Bianca
authors.initials: B
authors.lastname: Ferreira-Silva
authors.forename: Anton
authors.initials: A
authors.lastname: Glieder
authors.forename: Walter M F
authors.initials: WM
authors.lastname: Fabian
authors.forename: Stefaan
authors.initials: S
authors.lastname: de Wildeman
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kroutil
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D000438:Alcohols / Q000737:chemistry*; D020561:Alphaproteobacteria / Q000201:enzymology; D002384:Catalysis; D006859:Hydrogen / Q000737:chemistry*; D007659:Ketones / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D013997:Time Factors
chemical_list: D000438:Alcohols; D007659:Ketones; D006859:Hydrogen; D000426:Alcohol Dehydrogenase