title: Alteration of gene expression induced by Silurus asotus lectin in Burkitt's lymphoma cells.
abstract: Silurus asotus lectin (SAL) is a member of the rhamnose-binding lectin (RBL) family, and recognizes globotriaosylceramide (Gb3) on the cell surface of Burkitt's lymphoma cell lines, such as Raji and Daudi cells. The variation of gene expression in the treatment of both cells with SAL was analyzed using the differential display (DD) method with combination of 16 kinds of arbitary primers and 3 kinds of anchor primers. Treatment of Raji cells with SAL down-regulated mitochondria-associated granulocyte-macropharge colony-stimulating factor (GM-CSF) signaling molecule (Magmas) gene, and up-regulated N-myc downstream regulated gene (NDRG) 3. On the other hand, treatment of Daudi cells with SAL down-regulated Rad50 gene. Since Magmas gene expression was repressed in SAL-treated Raji cells, but did not change in SAL-treated D-threo-1-phenyl-2-decanoylamino-3-morphorino-1-propanol (PDMP)-pretreated Raji cells, it was clear that the expression of Magmas, NDRG3 or Rad50 was regulated by SAL binding to Gb3.
authors.affiliation: Division of Cell Recognition Study, Institute of Molecular Biomembrane and Glycobiolgy, Tohoku Pharmaceutical University, 4-4-1 Komatsushima, Aoba-ku, Sendai 981-8558, Japan.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Kawano
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sugawara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Hosono
authors.forename: Takeo
authors.initials: T
authors.lastname: Tatsuta
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nitta
mesh_terms: D017766:Acid Anhydride Hydrolases; D000818:Animals; D001483:Base Sequence; D002051:Burkitt Lymphoma / Q000235:genetics*; D002397:Catfishes / Q000378:metabolism*; D045744:Cell Line, Tumor; D002852:Chromatography, Ion Exchange; D017931:DNA Primers; D045643:DNA Repair Enzymes / Q000096:biosynthesis / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics; D030541:Databases, Genetic; D015870:Gene Expression / Q000187:drug effects; D016259:Genes, myc / Q000235:genetics; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000235:genetics; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D037102:Lectins / Q000633:toxicity*; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins / Q000096:biosynthesis / Q000235:genetics; D008969:Molecular Sequence Data; D009153:Mutagens; D009419:Nerve Tissue Proteins / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014281:Trihexosylceramides / Q000096:biosynthesis / Q000235:genetics
chemical_list: D017931:DNA Primers; D004268:DNA-Binding Proteins; D047908:Intracellular Signaling Peptides and Proteins; D037102:Lectins; D000090622:Mitochondrial Precursor Protein Import Complex Proteins; D024101:Mitochondrial Proteins; D009153:Mutagens; C431706:NDRG3 protein, human; D009419:Nerve Tissue Proteins; C503439:PAM16 protein, human; D012333:RNA, Messenger; D014281:Trihexosylceramides; C018549:globotriaosylceramide; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D017766:Acid Anhydride Hydrolases; C112158:RAD50 protein, human; D045643:DNA Repair Enzymes