title: Development of foreign body giant cells in response to implantation of Spongostan as a scaffold for cartilage tissue engineering.
abstract: Spongostan, a gelatinous haemostatic sponge, is used in surgery. Moreover, Spongostan may serve as a scaffold for proteins or cells implanted into defects. At the site of biomaterial implantation, foreign body giant cells (FBGCs) may develop which are responsible for Spongostan degradation. The purpose of the present study was to examine whether Spongostan may serve as a scaffold in allogenic grafting of chondrocytes developed from rabbit auricular cartilage. The obtained results indicate that Spongostan fulfils its function as a cell scaffold, induces no inflammatory reaction and involves development of foreign body giant cells which participate in the process of its degradation. Microscopic observation showed that FBGCs manifest presence of cytoplasmic projections and lysosomes, which participate in phagocytosis of the applied scaffold.
authors.affiliation: Department of Histology and Embryology, University School of Medicine, Wroclaw, Poland. marcel@hist.am.wroc.pl
authors.forename: Marek
authors.initials: M
authors.lastname: Cegielski
authors.forename: Ilona
authors.initials: I
authors.lastname: Izykowska
authors.forename: Marzena
authors.initials: M
authors.lastname: Podhorska-Okolow
authors.forename: Maciej
authors.initials: M
authors.lastname: Zabel
authors.forename: Piotr
authors.initials: P
authors.lastname: Dziegiel
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects; D002356:Cartilage / Q000601:surgery*; D019902:Chondrocytes / Q000378:metabolism; D005260:Female; D005339:Fibrin Foam / Q000009:adverse effects*; D015743:Giant Cells, Foreign-Body / Q000473:pathology* / Q000648:ultrastructure; D011817:Rabbits; D013997:Time Factors; D014014:Tissue Adhesives / Q000009:adverse effects*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D005339:Fibrin Foam; D014014:Tissue Adhesives