title: Antifungal activity of the plant Dodonaea viscosa var. angustifolia on Candida albicans from HIV-infected patients.
abstract: was to determine the minimum inhibitory concentration (MIC) and the time taken by Dodonaea viscosa var. angustifolia (PLE) a South African medicinal plant, chlorhexidine gluconate (CHX) and triclosan (TRN) to kill Candida albicans. 41 strains of Candida albicans were investigated, 20 from HIV-positive patients, 20 from HIV-negative subjects and Candida albicans ATCC 90028. The MICs of an acetone extract of PLE, CHX and TRN were measured using a microtitre double dilution technique, and the time taken to kill 99.5% of the strains was determined. The MICs of PLE, CHX and TRN were 6.25-25, 0.008-0.16 and 0.0022-0.009 mg/ml, respectively. PLE killed all the test strains within 30s and CHX 40% of the isolates from HIV-positive patients and 20% of strains from HIV-negative subjects in 1 min. During the same time TRN killed 55% and 35% of isolates from HIV-positive and HIV-negative patients. Dodonaea viscosa var. angustifolia has antifungal properties and is more effective than commercially available mouthrinses.
authors.affiliation: Division of Oral Microbiology, Department of Clinical Microbiology and Infectious Diseases, Faculty of Health Sciences, University of the Witwatersrand, Wits, Johannesburg, South Africa. mrudula.patel@wits.ac.za
authors.forename: Mrudula
authors.initials: M
authors.lastname: Patel
authors.forename: Maeve M
authors.initials: MM
authors.lastname: Coogan
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000188:drug therapy / Q000382:microbiology; D000891:Anti-Infective Agents, Local / Q000008:administration & dosage / Q000494:pharmacology; D000935:Antifungal Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D002176:Candida albicans / Q000187:drug effects*; D002177:Candidiasis / Q000188:drug therapy / Q000209:etiology; D002710:Chlorhexidine / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D015658:HIV Infections / Q000150:complications; D006801:Humans; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D029631:Sapindaceae / Q000737:chemistry*; D014260:Triclosan / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000891:Anti-Infective Agents, Local; D000935:Antifungal Agents; D010936:Plant Extracts; D014260:Triclosan; C010882:chlorhexidine gluconate; D002710:Chlorhexidine