Two-step modification of poly(D, L-lactic acid) by ethylenediamine-maleic anhydride.
Poly(lactic acid) (PLA) was modified by maleic anhydride (MAH), then the resultant MAH modified PLA (MPLA) was acylated with ethylenediamine (EDA), so EDA-MAH modified PLA (EMPLA) was prepared. The results of DSC, FT-IR and NMR testified that MAH and EAD were successfully introduced into the original polymer. The hydrophilicity of EMPLA was considerably increased compared with that of PLA. The degradation experiment showed that the introduction of EDA into the original polymer could neutralize the carboxyl end groups of the degradation products. The results of SEM and MTT of rat osteoblasts cultured in vitro showed that the cytocompatibility and cell adhesion of the modified materials were significantly increased compared with the original polymer, especially EMPLA; the number of cells were obviously increased and cells attached firmly to the material; these were ascribed to the EDA neutralizing the carboxyl end groups of the degradation products.
Cao
Chengbo
C
School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, People's Republic of China. cbcao@sdu.edu.cn
Zhu
Fanglian
F
Yu
Xueli
X
Wang
Qin
Q
Wang
Chuandong
C
Li
Baolu
B
Lv
Ronghui
R
Li
Musen
M
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002478:Cells, Cultured; D005029:Ethylenediamines / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D019344:Lactic Acid / Q000737:chemistry*; D008298:Maleates / Q000737:chemistry*; D008422:Materials Testing; D010006:Osteoblasts / Q000187:drug effects* / Q000502:physiology*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D051381:Rats; D013499:Surface Properties
D001672:Biocompatible Materials; D005029:Ethylenediamines; D008298:Maleates; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); C031234:ethylenediamine; C030272:maleic acid