[Experimental study of hepatic bloodflow to the effect of cryoablation].
OBJECTIVE
To investigate the bloodflow of hepetatic artery to the effect of cryoablation.


METHODS
Twenty dogs were divided into four groups randomly according to hepatic artery blood flow: completely occlusion of the hepatic artery(A), partial occlusion of the hepatic artery(B), increasing the hepatic artery blood flow(C) ,and no occlusion(D). Two areas of necrosis near portal hepatis (a)and far from it (b)were created in each of the 20 dog livers. Ultrasound was used to measure the blood flow. After cryoablation, the animals were euthanized, the livers were examined and the cryoablation areas were separated into two halves the maximum diameter of necrosis was assessed by vital staining for nicotinamide adenine dinucleotide (NADH) diaphorase and Triphenyl tetrazolium chloride (TTC) staining.


RESULTS
The peak speed and volflow of hepatic artery from group A to group D measured by ultrasound were 0 cm/s and 0 mL/min, (22.8+/-0.6) cm/s and (31.4+/-0.9) mL/min, (99.6+/-1.6) cm/s and (70.4+/-1.5) mL/min and (69.5+/-0.7) cm/s and (52.6+/-1.2) mL/min, respectively. The mean longest diameter of ice-ball in areas a from group A to group D were (4.5+/-0.4) cm,(3.5+/-0.1) cm,(2.0+/-0.3) cm,(2.6+/-0.2) cm and the areas b were (4.6+/-0.3) cm,(3.6+/-0.2) cm,(1.9+/-0.1) cm,(2.6+/-0.2) cm; the mean longest necroseis diameter in TTC staining in areas a were (4.0+/-0.3) cm,(2.7+/-0.2) cm, (2.0+/-0.2) cm, (2.3+/-0.1) cm, and in areas b were (3.9+/-0.3) cm, (2.6+/-0.3) cm, (2.1+/-0.1) cm,(2.5+/-0.2) cm; the mean longest necroseis diameter in NADH staining in areas a were (4.1+/-0.4) cm,(2.6+/-0.2) cm,(2.1+/-0.2) cm,(2.3+/-0.3) cm, in areas b were (4.0+/-0.3) cm, (2.7+/-0.3) cm,(2.1+/- 0.1) cm,(2.4+/-0.2) cm.


CONCLUSIONS
The bloodflow of hepetatic artery can affect the cryoablation rate. When the bloodflow increases, the cryoablation rate decreases.
Guo
Zhi
Z
Intervention Radiation Department, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.
Ni
Hong
H
Hu
Yong hua
Yh
Xing
Wen ge
Wg
Liu
Fang
F
D000818:Animals; D001783:Blood Flow Velocity; D003452:Cryosurgery; D004285:Dogs; D005260:Female; D006499:Hepatic Artery / Q000601:surgery*; D008099:Liver / Q000098:blood supply* / Q000473:pathology / Q000601:surgery*; D008297:Male; D009336:Necrosis; D011897:Random Allocation