Toxic effects of acrylamide on survival, development and haemocytes of Musca domestica.
The influence of acrylamide, a potentially toxic substance present in some types of food, on survival, postembryonic development and haemocytes, insect's blood cells, of the housefly was examined. Larvae were reared on media contaminated with acrylamide at concentrations of 82 microg/g, 164 microg/g or 246 microg/g. The length of larval and pupal stages as well as the survival of larvae and pupae was examined. To study the effects of acrylamide on haemocytes, the analysis of their index and morphology was performed in the third instar larva. The obtained data showed that the survival of larvae exposed to 82 microg/g and 164 microg/g concentrations of acrylamide decreased by 50% and 85%, respectively, whereas 246 microg/g concentration was lethal. In both groups of flies, larval and pupal stages were significantly lengthened by about 1.5 day in comparison with control. Moreover, acrylamide increased the number of prohaemocytes and intermediate cells while the number of plasmatocytes and granulocytes decreased. The size of plasmatocytes decreased in acrylamide-treated larvae when compared with these cells of control flies. The reduced survival of animals is probably due to affecting haemocytes involved in immune responses in insects. Moreover, the housefly's blood cells showed to be sensitive to toxin, which suggests their usefulness to test toxicity of substances present in food products.
Szczerbina
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Department of Cytology and Histology, Institute of Zoology, Jagiellonian University, Ingardena 6, 30-060 Kraków, Poland.
Banach
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Tylko
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Pyza
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D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D048429:Cell Size / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D006434:Hemocytes / Q000187:drug effects*; D006793:Houseflies / Q000187:drug effects* / Q000196:embryology / Q000254:growth & development; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D007928:Lethal Dose 50; D011679:Pupa / Q000187:drug effects / Q000254:growth & development
D020106:Acrylamide