Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions.
We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.
Schulte
Roberta
R
Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, United States of America.
Talamas
Jessica
J
Doucet
Christine
C
Hetzer
Martin W
MW
D000818:Animals; D002846:Chromatography, Affinity / Q000379:methods*; D004247:DNA / Q000378:metabolism; D006801:Humans; D025941:Protein Interaction Mapping / Q000379:methods*; D011506:Proteins / Q000378:metabolism*; D014981:Xenopus
D011506:Proteins; D004247:DNA