Compression and crevasses in vitreous sections under different cutting conditions.
Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.
Han
H-M
HM
Laboratoire d'Analyse Ultrastructurale, Bâtiment de Biologie, Université de Lausanne, CH-1015 Lausanne, Switzerland. hongmei.han@yahoo.com.cn
Zuber
B
B
Dubochet
J
J
D016477:Artifacts; D020285:Cryoelectron Microscopy / Q000379:methods*; D016616:Cryoultramicrotomy / Q000295:instrumentation* / Q000379:methods; D013293:Enterococcus faecalis / Q000254:growth & development / Q000648:ultrastructure*; D005629:Frozen Sections