title: 17beta-estradiol affects proliferation and apoptosis of rat prostatic smooth muscle cells by modulating cell cycle transition and related proteins.
abstract: Abundant evidence indicates that estrogens have an important role in the pathology of benign prostatic hyperplasia (BPH). To investigate the effect of 17beta-estradiol (E2) on the proliferation and apoptosis of prostatic smooth muscle cells (PSMCs), rat PSMCs were obtained and exposed to gradient concentrations (0.1-100 nmol/l) of E2 over varying amounts of time. The progression of cell cycle, cellular apoptosis, cyclin D1, Bcl-2 and Bax proteins were detected. The data show that the effect of E2 on rat PSMCs is bilateral: it promotes cell proliferation by enhancing the expression of cyclin D1, which accelerates G1 to S phase transition; on the other hand, it induces apoptosis of the cells by up-regulating the expression of Bax. We thus suggest that an increase in estrogen may exert a launching effect in the pathology of BPH.
authors.affiliation: Research Center of Urology and Andrology, Zhongnan Hospital, Wuhan University, 169# Donghu Road, Wuhan, Hubei 430071, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Luo
authors.forename: Wafi
authors.initials: W
authors.lastname: Waladali
authors.forename: Shiwen
authors.initials: S
authors.lastname: Li
authors.forename: Xinmin
authors.initials: X
authors.lastname: Zheng
authors.forename: Liquan
authors.initials: L
authors.lastname: Hu
authors.forename: Hang
authors.initials: H
authors.lastname: Zheng
authors.forename: Wanli
authors.initials: W
authors.lastname: Hu
authors.forename: Chan
authors.initials: C
authors.lastname: Chen
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D019938:Cyclin D1 / Q000187:drug effects / Q000378:metabolism; D004958:Estradiol / Q000494:pharmacology*; D008297:Male; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011467:Prostate / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000187:drug effects / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation; D051028:bcl-2-Associated X Protein / Q000187:drug effects / Q000378:metabolism
chemical_list: D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D019938:Cyclin D1; D004958:Estradiol