title: Peripheral vascular disease: who gets it and why? A histomorphological analysis of 261 arterial segments from 58 cases.
abstract: OBJECTIVE
  This retrospective study aimed to document and illustrate the histomorphological changes underlying peripheral vascular disease (PVD). More specifically, it aimed to analyse and quantify those changes that lead to lower limb amputations. Histological changes were assessed in relation to various clinical pathologies, and significant correlations were sought thereafter.
  
  
  METHODS
  A total of 1305 arterial segments were examined from 58 consecutive patients undergoing a lower limb amputation from January 2002 to December 2003. Serial arterial segments were taken from the femoral, popliteal, anterior tibial, posterior tibial, peroneal, and dorsalis pedis arteries, and the degrees of atherosclerotic stenosis and medial calcification were histologically quantified.
  
  
  RESULTS
  Atherosclerosis was associated with severe arterial stenosis. An increased occurrence of severe atherosclerotic narrowing coincided with increasing patient age (p = 0.0166), hypertension (p = 0.0019), and diabetes mellitus (p = 0.0036). The presence of medial calcification was an important pathological feature in patients under 70 years of age (p = 0.0308) and significantly more severe in those with diabetes mellitus (p<0.001).
  
  
  CONCLUSIONS
  Atherosclerosis and medial calcification are significant underlying lesions in diabetic patients undergoing lower limb amputation. Medial calcification can cause significant stiffening of the arterial wall and a reduction in its ability to respond to vasodilator stimuli.
authors.affiliation: Department of Pathology,Toronto General Hospital/University Health Network, Toronto, Ontario, Canada.
authors.forename: Gursharan S
authors.initials: GS
authors.lastname: Soor
authors.forename: Iva
authors.initials: I
authors.lastname: Vukin
authors.forename: Shaun W
authors.initials: SW
authors.lastname: Leong
authors.forename: George
authors.initials: G
authors.lastname: Oreopoulos
authors.forename: Jagdish
authors.initials: J
authors.lastname: Butany
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000671:Amputation, Surgical; D001158:Arteries / Q000473:pathology; D050197:Atherosclerosis / Q000150:complications / Q000378:metabolism / Q000473:pathology; D002114:Calcinosis / Q000378:metabolism / Q000473:pathology; D003251:Constriction, Pathologic / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000473:pathology; D003925:Diabetic Angiopathies / Q000150:complications / Q000473:pathology; D005260:Female; D006801:Humans; D007511:Ischemia / Q000473:pathology; D007866:Leg / Q000098:blood supply*; D008297:Male; D008875:Middle Aged; D016491:Peripheral Vascular Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D012189:Retrospective Studies; D017540:Tunica Media / Q000378:metabolism / Q000473:pathology