Domain organization of photosystem II in membranes of the cyanobacterium Synechocystis PCC6803 investigated by electron microscopy.
The supramolecular organization of photosystem II (PSII) complexes in the photosynthetic membrane of the cyanobacterium Synechocystis 6803 was studied by electron microscopy. After mild detergent solubilization, crystalline PSII arrays were extracted in which dimeric PSII particles associate in multiple rows. Image processing of the arrays shows that the PSII dimers are tightly packed at distances of 12.2 and 16.7 nm. The domains are considered to be an important type of association for preventing either spill-over energy from PSII towards photosystem I (PSI) or direct energy flow from phycobilisomes to PSI, because the latter can only be at periphery of the arrays.
Folea
I Mihaela
IM
Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands.
Zhang
Pengpeng
P
Aro
Eva-Mari
EM
Boekema
Egbert J
EJ
D001426:Bacterial Proteins / Q000737:chemistry*; D019281:Dimerization; D008854:Microscopy, Electron; D045332:Photosystem II Protein Complex / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D012995:Solubility; D046939:Synechocystis / Q000201:enzymology*; D020524:Thylakoids / Q000201:enzymology / Q000648:ultrastructure
D001426:Bacterial Proteins; D045332:Photosystem II Protein Complex