Prognostic value of a proliferation index including MIB1 and argyrophilic nucleolar organizer regions proteins in node-negative breast cancer.
OBJECTIVE
This study was designed to evaluate a cell proliferation marker, including the percentage of cycling cells (MIB1), and the duration of the cell cycle (assessed by argyrophilic nucleolar organizer regions proteins [AgNORs] measurement).


METHODS
We included 90 patients with invasive node-negative breast cancer. None received chemotherapy. With the help of a double-staining technique, a proliferation index (PI) was determined by multiplying the percentage of MIB1-positive cells by the mean area of the AgNORs present in those MIB1-positive cells. PI was evaluated for its impact on overall survival (OS) and disease-free survival (DFS).


RESULTS
We demonstrated that PI was correlated to OS. For DFS, it conserved its high prognostic value only in univariate analysis. The global amount of AgNORs was more discriminative for DFS.


CONCLUSIONS
PI and AgNOR quantification supplied additional prognosis information in node-negative patients, and we propose to integrate them in further studies.
Abboud
Pascal
P
Department of Gynecology and Obstetrics, Hospital of Soissons, Soissons, France. pascal.abboud@ch-soissons.fr
Lorenzato
Marianne
M
Joly
Damien
D
Quereux
Christian
C
Birembaut
Philippe
P
Ploton
Dominique
D
D000368:Aged; D034961:Antigens, Nuclear / Q000032:analysis*; D001943:Breast Neoplasms / Q000401:mortality* / Q000503:physiopathology / Q000601:surgery; D002467:Cell Nucleus / Q000737:chemistry*; D049109:Cell Proliferation; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D015999:Multivariate Analysis; D009363:Neoplasm Proteins; D011379:Prognosis; D044767:Ubiquitin-Protein Ligases / Q000032:analysis*
D034961:Antigens, Nuclear; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; C051963:nucleolar organizer region associated proteins; C513496:MIB1 ligase, human; D044767:Ubiquitin-Protein Ligases