Protective effects of an extract of young radish (Raphanus sativus L) cultivated with sulfur (sulfur-radish extract) and of sulforaphane on carbon tetrachloride-induced hepatotoxicity.
The protective effects of an extract of young radish (Raphanus sativus L) cultivated with sulfur (sulfur-radish extract) and of sulforaphane, an isothiocyanate, on carbon tetrachloride (CCl(4))-induced liver injury were observed in mice. CCl(4) produced a marked increase in the serum level of alanine aminotransferase (ALT), primed lipid peroxidation, and resulted in intense necrosis due to oxidative stress. Oral administration of the sulfur-radish extract and of sulforaphane after CCl(4)-induced liver injury both decreased the serum level of ALT, reduced the necrotic zones, inhibited lipid peroxidation, and induced phase 2 enzymes without affecting cytochrome P450-2E1 (CYP2E1). These results suggest that the administration of the sulfur-radish extract and of sulforaphane may partially prevent CCl(4)-induced hepatotoxicity, possibly by indirectly acting as an antioxidant by improving the detoxification system.
Baek
Seung-Hae
SH
Department of Biological Sciences and Immunomodulation Research Center, University of Ulsan, Ulsan 680-749, Korea.
Park
Min
M
Suh
Jae-Hee
JH
Choi
Hye-Seon
HS
D000410:Alanine Transaminase / Q000097:blood / Q000378:metabolism; D000818:Animals; D002251:Carbon Tetrachloride / Q000633:toxicity*; D005982:Glutathione Transferase / Q000378:metabolism; D017879:Isothiocyanates; D008099:Liver / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D031224:Raphanus / Q000737:chemistry*; D013454:Sulfoxides; D013455:Sulfur / Q000737:chemistry*; D013858:Thiobarbiturates / Q000378:metabolism; D013861:Thiocyanates / Q000494:pharmacology*
D017879:Isothiocyanates; D010936:Plant Extracts; D013454:Sulfoxides; D013858:Thiobarbiturates; D013861:Thiocyanates; D013455:Sulfur; D002251:Carbon Tetrachloride; D016660:NAD(P)H Dehydrogenase (Quinone); D005982:Glutathione Transferase; D000410:Alanine Transaminase; C016766:sulforaphane; C029684:thiobarbituric acid