High rates of "unselected" aneuploidy and chromosome rearrangements in tel1 mec1 haploid yeast strains.
The yeast TEL1 and MEC1 genes (homologous to the mammalian ATM and ATR genes, respectively) serve partially redundant roles in the detection of DNA damage and in the regulation of telomere length. Haploid yeast tel1 mec1 strains were subcultured nonselectively for approximately 200 cell divisions. The subcultured strains had very high rates of chromosome aberrations: duplications, deletions, and translocations. The breakpoints of the rearranged chromosomes were within retrotransposons (Ty or delta-repeats), and these chromosome aberrations nonrandomly involved chromosome III. In addition, we showed that strains with the hypomorphic mec1-21 allele often became disomic for chromosome VIII. This property of the mec1-21 strains is suppressed by a plasmid containing the DNA2 gene (located on chromosome VIII) that encodes an essential nuclease/helicase involved in DNA replication and DNA repair.
Vernon
Michael
M
Neuroscience Center, University of North Carolina, Chapel Hill, NC 27599, USA.
Lobachev
Kirill
K
Petes
Thomas D
TD
D000782:Aneuploidy; D002869:Chromosome Aberrations; D017931:DNA Primers / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D046228:Microarray Analysis; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013045:Species Specificity
D017931:DNA Primers; D047908:Intracellular Signaling Peptides and Proteins; D029701:Saccharomyces cerevisiae Proteins; C088937:MEC1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C513623:TEL1 protein, S cerevisiae