Emticicia ginsengisoli sp. nov., a species of the family 'Flexibacteraceae' isolated from soil of a ginseng field.
A taxonomic study was carried out on Gsoil 085T, a bacterial strain isolated from a soil sample from a ginseng field in Pocheon Province (South Korea). Comparative 16S rRNA gene sequence studies showed a clear affiliation of this bacterium to the family 'Flexibacteraceae' and indicated that the closest relative was Emticicia oligotrophica GPTSA100-15T (94.6 % sequence similarity). Lower sequence similarities (<83.0 %) were found with respect to all other recognized species of the family 'Flexibacteraceae'. Strain Gsoil 085T was found to be Gram-negative, strictly aerobic, non-motile and rod-shaped. It grew well on half-strength R2A medium but did not utilize a broad range of carbon sources. The G+C content of the genomic DNA was 40.5 mol%. The major fatty acids were summed feature 4 (iso-C15 : 0 2-OH and/or C16 : 1omega7c, 45.4 %), iso-C15 : 0 (13.9 %), C16 : 1omega5c (8.6 %) and C16 : 0 (8.2 %). On the basis of evidence from the polyphasic study, strain Gsoil 085T represents a novel species of the genus Emticicia, for which the name Emticicia ginsengisoli sp. nov. is proposed. The type strain is Gsoil 085T (=KCTC 12588T =LMG 23396T).
Liu
Qing-Mei
QM
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1 Guseong-dong, Yuseong-gu, Daejeon 305-701, Republic of Korea.
Ten
Leonid N
LN
Yu
Hong-Shan
HS
Jin
Feng-Xie
FX
Im
Wan-Taek
WT
Lee
Sung-Taik
ST
D015373:Bacterial Typing Techniques; D001482:Base Composition; D041964:Cytophagaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D007723:Korea; D008969:Molecular Sequence Data; D005894:Panax; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S