title: Upregulation by KCl treatment of eukaryotic translation elongation factor 1A (eEF1A) mRNA in the dendrites of cultured rat hippocampal neurons.
abstract: Activity-dependent local translation in the dendrites of brain neurons plays an important role in the synapse-specific provision of proteins necessary for strengthening synaptic connections. In this study we carried out combined fluorescence in situ hybridization (FISH) and immunocytochemistry (IC) and showed that more than half of the eukaryotic elongation factor 1A (eEF1A) mRNA clusters overlapped with or were immediately adjacent to clusters of PSD-95, a postsynaptic marker, in the dendrites of cultured rat hippocampal neurons. Treatment of the neurons with KCl increased the density of the dendritic eEF1A mRNA clusters more than two-fold. FISH combined with IC revealed that the KCl treatment increased the density of eEF1A mRNA clusters that overlapped with or were immediately adjacent to PSD-95 clusters. These results indicate that KCl treatment increases both the density of eEF1A mRNA clusters and their synaptic association in dendrites of cultured neurons.
authors.affiliation: Department of Anatomy, College of Medicine, Dongguk University, Gyeongju 780-714, Korea. moonis@dongguk.ac.kr
authors.forename: Il Soo
authors.initials: IS
authors.lastname: Moon
authors.forename: Sun-Jung
authors.initials: SJ
authors.lastname: Cho
authors.forename: Hyunsook
authors.initials: H
authors.lastname: Lee
authors.forename: Dae-Hyun
authors.initials: DH
authors.lastname: Seog
authors.forename: Yong Wook
authors.initials: YW
authors.lastname: Jung
authors.forename: Ingnyol
authors.initials: I
authors.lastname: Jin
authors.forename: Randall
authors.initials: R
authors.lastname: Walikonis
mesh_terms: D000818:Animals; D001693:Biological Transport, Active / Q000187:drug effects / Q000235:genetics; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D003712:Dendrites / Q000235:genetics / Q000378:metabolism*; D000076122:Disks Large Homolog 4 Protein; D006624:Hippocampus / Q000166:cytology*; D017404:In Situ Hybridization, Fluorescence; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D009474:Neurons / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D011189:Potassium Chloride / Q000494:pharmacology; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D051381:Rats; D017207:Rats, Sprague-Dawley; D013570:Synaptic Membranes / Q000648:ultrastructure; D015854:Up-Regulation
chemical_list: D004268:DNA-Binding Proteins; D000076122:Disks Large Homolog 4 Protein; C501449:Dlg4 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D012333:RNA, Messenger; C085420:enhancer factor 1(A), rat; D011189:Potassium Chloride