title: Production, purification and characterization of beta-1,4-endoglucanase from a novel bacterial strain CTP-09 of a Bacillus sp.
abstract: Bacillus strain CTP-09 yielded maximum productivity (1120 IU/L.h) of extracellular endoglucanase (CMCase) on 0.5% cellobiose after 10 h fermentation at 55 degrees C. The purified enzyme is mono-meric in nature and exhibits stability up to 80 degrees C and over a pH range (6.0-9.0). Activation energy, enthalpy and entropy of catalysis, and inactivation indicated that this CMCase is highly thermos-table. Purified enzyme possessed high power of defibrillation of textile and was minutely inhibited by anionic detergent and oxidizing agent comparable with inhibition by commercial enzyme. This polypeptide could be exploited for mass production and application in local industries.
authors.affiliation: National Institute of Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
authors.forename: Mahjabeen
authors.initials: M
authors.lastname: Saleem
authors.forename: Muhammad Saleem
authors.initials: MS
authors.lastname: Akhtar
authors.forename: Riffat
authors.initials: R
authors.lastname: Yasmin
authors.forename: Mahreen
authors.initials: M
authors.lastname: Zahid
authors.forename: Nadeem Nawazish
authors.initials: NN
authors.lastname: Malik
authors.forename: Munazza
authors.initials: M
authors.lastname: Afzal
authors.forename: Muhammad Ibrahim
authors.initials: MI
authors.lastname: Rajoka
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D002480:Cellulase / Q000037:antagonists & inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D004789:Enzyme Activation; D007700:Kinetics; D007846:Laundering; D008970:Molecular Weight; D011489:Protein Denaturation; D013379:Substrate Specificity; D013696:Temperature; D013783:Textile Industry; D013816:Thermodynamics
chemical_list: D002480:Cellulase