title: Formation of an ascorbate-apatite composite layer on titanium.
abstract: An ascorbate-apatite composite layer was successfully formed on NaOH- and heat-treated titanium by coprecipitating L-ascorbic acid phosphate and low-crystalline apatite in a supersaturated calcium phosphate solution at 37 degrees C for 48 h. The supersaturated calcium phosphate solutions used have chemical compositions attainable by mixing infusion fluids officially approved for clinical use. The amount of immobilized L-ascorbic acid phosphate ranged from 1.0 to 2.3 microg mm(-2), which is most likely to be sufficient for the in vitro osteogenic differentiation of mesenchymal stem cells on titanium. Since ascorbate is important for the collagen synthesis and subsequent osteogenesis of mesenchymal stem cells, titanium coated with the ascorbate-apatite composite layer would be useful as a scaffold in bone tissue engineering and as a bone substitute.
authors.affiliation: National Institute of Advanced Industrial Science and Technology (AIST), Institute for Human Science and Biomedical Engineering, Central 6, Higashi 1-1-1, Tsukuba, Ibaraki 305-8566, Japan. atsuo-ito@aist.go.jp
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ito
authors.forename: Yu
authors.initials: Y
authors.lastname: Sogo
authors.forename: Yuko
authors.initials: Y
authors.lastname: Ebihara
authors.forename: Masahiro
authors.initials: M
authors.lastname: Onoguchi
authors.forename: Ayako
authors.initials: A
authors.lastname: Oyane
authors.forename: Noboru
authors.initials: N
authors.lastname: Ichinose
mesh_terms: D001031:Apatites / Q000737:chemistry*; D001205:Ascorbic Acid / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003460:Crystallization / Q000379:methods*; D008422:Materials Testing; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001031:Apatites; D018786:Bone Substitutes; D020099:Coated Materials, Biocompatible; D014025:Titanium; D001205:Ascorbic Acid