A study on differentially expressed gene screening of Chrysanthemum plants under sound stress.
Environmental stress can induce differential expression of genes of flower plants. It had been found that sound stimulation had an obvious effect on the growth and development of flower plants, but it is not reported on the differentially expressed genes and their expressing characteristics under sound stimulation. This is one of the few reports in terms of using the DDRT-PCR technique for screening the differentially expressed cDNA fragments responding to sound-wave stress on Chrysanthemum. Six differentially expressed cDNA fragments were obtained. Molecular weight of fragments was from 200 to 600 bp, respectively. Among differential fragments acquired, three of them (SA3, SG7-1, and CA2) were found to be positive fragments by northern dot hybridization, whose molecular weight are 270, 580 and 370 bp, respectively. SA3 was differentially expressed and SG7-1 was preferably expressed, while CA2 was restrained by the sound wave. These results indicated that expression of some genes was turned on, meanwhile the stress restrained some genes from expression under the mode of sound-stress stimulation.
Hongbo
Shao
S
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Sciences, Northwest A&F University, Yangling 712100, China. shaohongbochu@126.com
Biao
Li
L
Bochu
Wang
W
Kun
Tang
T
Yilong
Liang
L
D015152:Blotting, Northern; D002478:Cells, Cultured; D002847:Chromatography, Agarose; D031202:Chrysanthemum / Q000235:genetics* / Q000502:physiology*; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D018744:DNA, Plant / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000502:physiology*; D009622:Noise / Q000009:adverse effects*; D018547:Plant Stems / Q000166:cytology / Q000378:metabolism; D018749:RNA, Plant / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016622:Silver Staining; D013016:Sound / Q000009:adverse effects*; D013056:Spectrophotometry, Ultraviolet
D018076:DNA, Complementary; D018744:DNA, Plant; D018749:RNA, Plant