[CIAPIN1 expression in human lung cancer tissues and inhibitory effects of the gene on human pulmonary carcinoma NCI-H446 cells].
OBJECTIVE
To explore CIAPIN1 gene expression in lung carcinoma tissues and its regulatory in the growth of NCI-H446.


METHODS
Fifty-one formalin-fixed paraffin-embedded specimens of primary lung cancer and their non-cancerous counterparts were detected by SABC immunohistochemistry method. Adenoviral vector construction was recombined and the gene was transduced into NCI-H446 cells. The expression of CIAPIN1 protein was identified by Western blot. Trypan blue staining was used to count the alive cells and to draw a cellular growth curve. The changes of cell cycle and apoptosis were analyzed by flow cytometry.


RESULTS
The positive rate of CIAPIN1 expression in cancer tissues (39.2%)was much lower than that in non-cancerous counterparts (100%, P<0.05). In Ad-CIAPIN1 group, the growth of transfected NCI-H446 cells in vitro was significantly inhibited. In addition the Ad-CIAPIN1-induced cell apoptosis and a predominant arrest in the G1/S phase (P<0.01) were observed.


CONCLUSIONS
The down-regulation of CIAPIN1 expression in tumor is associated with the development of lung carcinoma. Transduction of NCI-H446 CIAPIN1-negative cell, with Ad-CIAPIN1 can inhibit cell growth, suggesting that CIAPIN1 can be a new tumor-related suppressor gene.
Wang
Yang
Y
Department of Respiratory Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China. yangwang@fmmu.edu.cn
Qi
Hao-wen
HW
Li
Xiao-hua
XH
Chen
Xu-xin
XX
Liu
Jie
J
D000972:Antineoplastic Agents, Phytogenic; D002283:Carcinoma, Bronchogenic; D002453:Cell Cycle / Q000187:drug effects* / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000494:pharmacology; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000494:pharmacology; D015536:Down-Regulation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000502:physiology; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000494:pharmacology*; D008175:Lung Neoplasms / Q000737:chemistry* / Q000378:metabolism; D008869:Microtubule-Associated Proteins / Q000494:pharmacology; D034741:RNA, Small Interfering / Q000494:pharmacology; D055752:Small Cell Lung Carcinoma / Q000737:chemistry* / Q000378:metabolism; D025521:Tumor Suppressor Proteins / Q000494:pharmacology
D000972:Antineoplastic Agents, Phytogenic; C489114:CIAPIN1 protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D047908:Intracellular Signaling Peptides and Proteins; D008869:Microtubule-Associated Proteins; D034741:RNA, Small Interfering; D025521:Tumor Suppressor Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27