Gene expression in a canine basilar artery vasospasm model: a genome-wide network-based analysis.
To investigate the changes of gene expression on the cerebral vasospasm after subarachnoid hemorrhage, we used genome-wide microarray for a canine double-hemorrhage model and analyzed the data by using a network-based analysis. Six dogs were assigned to two groups of three animals: control and hemorrhage. The effects were assessed by the changes in gene expressions in the artery 7 days after the first blood injection. Among 23,914 genes, 447 and 66 genes were up-regulated more than two- and fivefold, respectively, and 332 and 25 genes were down-regulated more than two- and fivefold, respectively. According to gene ontology, genes related to cell communication (P = 5.28E-10), host-pathogen interaction (7.65E-8), and defense-immunity protein activity (0.000183) were significantly overrepresented. The top high-level function for the merged network derived from the network-based analysis was cell signaling, revealing that the subgroup that regulates the quantity of Ca(2+) to have the strongest association significance (P = 4.75E-16). Canine microarray analysis followed by gene ontology profiling and connectivity analysis identified several functional groups and individual genes responding to cerebral vasospasm. Ca(2+) regulation may play a key role in these gene expression changes and may be involved in the pathogenesis of cerebral vasospasm.
Sasahara
Atsushi
A
Department of Neurosurgery, Tokyo Women's Medical University, Tokyo, Japan.
Kasuya
Hidetoshi
H
Krischek
Boris
B
Tajima
Atsushi
A
Onda
Hideaki
H
Sasaki
Toshiyuki
T
Akagawa
Hiroyuki
H
Hori
Tomokatsu
T
Inoue
Ituro
I
D000818:Animals; D001488:Basilar Artery / Q000503:physiopathology; D003627:Data Interpretation, Statistical; D004285:Dogs; D015536:Down-Regulation / Q000235:genetics / Q000502:physiology; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics; D006801:Humans; D016571:Neural Networks, Computer; D020411:Oligonucleotide Array Sequence Analysis; D015398:Signal Transduction / Q000235:genetics / Q000502:physiology; D013345:Subarachnoid Hemorrhage / Q000235:genetics; D020301:Vasospasm, Intracranial / Q000235:genetics* / Q000473:pathology