Transgenic sheep designed for transplantation studies.
Lentiviral vectors have recently emerged as an efficient method of transgene delivery to the germline of animals. We now demonstrate that combining this efficiency with embryo splitting procedures enables the production of monozygotic twins, one of which is transgenic. We propose that this approach can be used to generate animals in which cell or tissue transplantation can be achieved without the use of immunosuppressive regimes.
Ritchie
William A
WA
Roslin Institute, Animal Services Department, Roslin, Midlothian, UK.
King
Tim
T
Neil
Claire
C
Carlisle
Ailsa J
AJ
Lillico
Simon
S
McLachlan
Gerry
G
Whitelaw
C Bruce A
CB
D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D017690:Cell Transplantation / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D016086:Lentivirus / Q000235:genetics; D012756:Sheep / Q000235:genetics* / Q000378:metabolism*; D016378:Tissue Transplantation / Q000379:methods*; D019076:Transgenes / Q000235:genetics