title: Fingerprint of Hedyotis diffusa Willd. by HPLC-MS.
abstract: A HPLC-MS fingerprint method has been developed based on the consistent chromatographic features of the major chemical constituents among 10 batches of Hedyotis diffusa Willd. Chromatographic separation was conducted on a Hypersil-Keystone Hypurity C(18) column using methanol:water:acetic acid as the mobile phase. Major compounds, including oleanolic acid, ursolic acid and ferulic acid, were analysed by HPLC-MS. Their analysis was ascertained by comparison with data derived from the standard compounds. The HPLC-MS fingerprint was successfully applied to analyse and differentiate samples from different geographical origins, or processing methods. H. diffusa was well distinguished from Hedyotis chrysotricha by HPLC-MS. Therefore the establishment of fingerprint of H. diffusa is critical in assessing and controlling its overall quality.
authors.affiliation: School of Biosciences and Technologies, Central South University, Changsha, Hunan 410013, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Yang
authors.forename: Yi-Hua
authors.initials: YH
authors.lastname: Yang
authors.forename: Ju-Yun
authors.initials: JY
authors.lastname: Yang
authors.forename: Ben-Mei
authors.initials: BM
authors.lastname: Chen
authors.forename: Ju-Ping
authors.initials: JP
authors.lastname: Duan
authors.forename: Shu-Yi
authors.initials: SY
authors.lastname: Yu
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Ouyang
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Cheng
authors.forename: Yu-Xiang
authors.initials: YX
authors.lastname: Chen
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D032064:Hedyotis / Q000737:chemistry*; D013058:Mass Spectrometry; D015203:Reproducibility of Results
chemical_list: D004365:Drugs, Chinese Herbal