Fast differential interference contrast imaging combined with autocorrelation treatments to measure the heart rate of embryonic fish.
To develop an accurate and convenient method for measuring the heart rate of zebra fish in vivo, a system combining fast differential interference contrast (DIC) imaging with an autocorrelation technique is established. The imaging correlation coefficient corr(i,j) between frame i, selected from the obtained time-lapse imaging series as the reference image, and any other frame j, is calculated as the time-dependent cycle course. Heat rate is determined by the cycle period of the corr with a high temporal resolution of 4 ms, achieved by fast charge-coupled device (CCD) imaging of 250 frames per second. With this high-resolution system, we find that 1-mgL cadmium not only induces the slowing of the heart rate, but also caused signs of arrhythmia in treated fish.
Zhu
Jin-Tao
JT
Fudan University, Surface Physics Laboratory (National Key Laboratory) and Department of Physics, Shanghai 200433, China.
He
Jia
J
Chen
Ji-Yao
JY
Lu
Da-Ru
DR
Zhou
Lu-Wei
LW
D000465:Algorithms; D000818:Animals; D015148:Cardiotocography / Q000379:methods*; D006339:Heart Rate / Q000502:physiology*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D013223:Statistics as Topic; D015027:Zebrafish / Q000196:embryology* / Q000502:physiology*