Interaction between antioxidants in assays of total antioxidant capacity.
Sub-additivity of antioxidant activities in assays of total antioxidant capacity has been reported previously and ascribed to binding of low-molecular weight antioxidants such as flavonoids by proteins. We demonstrate that this phenomenon is much more common and concerns also interactions between typical low-molecular weight oxidants in the assays of ABTS- decolorization and protection of fluorescein from AAPH-induced bleaching. The subadditive interactions between antioxidants may affect quantitative considerations drawn from in vitro assays of antioxidant capacity of biological samples.
Błauz
Andrzej
A
Department of Molecular Biophysics, University of Łódź, ul. Banacha 12/16, 90-237 Łódź, Poland.
Pilaszek
Tomasz
T
Grzelak
Agnieszka
A
Dragan
Agata
A
Bartosz
Grzegorz
G
D000975:Antioxidants / Q000737:chemistry* / Q000378:metabolism*; D019540:Area Under Curve; D052160:Benzothiazoles / Q000032:analysis* / Q000378:metabolism; D004347:Drug Interactions; D004357:Drug Synergism; D005419:Flavonoids / Q000737:chemistry*; D019793:Fluorescein / Q000032:analysis* / Q000378:metabolism; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D008970:Molecular Weight; D010084:Oxidation-Reduction; D013451:Sulfonic Acids / Q000032:analysis* / Q000378:metabolism
D000975:Antioxidants; D052160:Benzothiazoles; D005419:Flavonoids; D016166:Free Radical Scavengers; D007202:Indicators and Reagents; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D019793:Fluorescein