Same-single-cell analysis for the study of drug efflux modulation of multidrug resistant cells using a microfluidic chip.
Since multidrug resistance (MDR) is a major cause of failure in cancer chemotherapy, we report a microfluidic approach combined with the same-single-cell analysis to investigate the modulation of MDR, manifested as the inhibition of drug efflux. A microfluidic chip that was capable of selecting and retaining a single multidrug-resistant cancer cell was used to investigate drug efflux inhibition in leukemia cell lines. Three advantages of the microfluidic-based same-single-cell analysis (dubbed as SASCA) method have been revealed. First, it readily detects the modulation of drug efflux of anticancer compounds (e.g., daunorubicin) by MDR modulators (e.g., verapamil) among cellular variations. Second, SASCA is able to compare the different cellular abilities in response to drug efflux modulation based on the drug transport kinetics of single cells. Third, SASCA requires only a small number of cells, which may be beneficial for investigating drug resistance in minor cell subpopulations (e.g., cancer "stem" cells).
Li
XiuJun
X
Department of Chemistry, Simon Fraser University, Burnaby, BC, V5A 1S6, Canada.
Ling
Victor
V
Li
Paul C H
PC
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000378:metabolism; D000970:Antineoplastic Agents / Q000378:metabolism / Q000494:pharmacology; D037621:Artemisinins / Q000378:metabolism / Q000494:pharmacology; D000077332:Artesunate; D045744:Cell Line, Tumor; D002477:Cells / Q000378:metabolism*; D047188:Chalcones / Q000378:metabolism / Q000494:pharmacology; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D006801:Humans; D008516:Medicine, Chinese Traditional; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D012717:Sesquiterpenes / Q000378:metabolism / Q000494:pharmacology; D014700:Verapamil / Q000378:metabolism
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D000970:Antineoplastic Agents; D037621:Artemisinins; D047188:Chalcones; D012717:Sesquiterpenes; D000077332:Artesunate; C040920:isoliquiritigenin; D014700:Verapamil