nuScore: a web-interface for nucleosome positioning predictions.
Sequence-directed mapping of nucleosome positions is of major biological interest. Here, we present a web-interface for estimation of the affinity of the histone core to DNA and prediction of nucleosome arrangement on a given sequence. Our approach is based on assessment of the energy cost of imposing the deformations required to wrap DNA around the histone surface. The interface allows the user to specify a number of options such as selecting from several structural templates for threading calculations and adding random sequences to the analysis.


BACKGROUND
The nuScore interface is freely available for use at http://compbio.med.harvard.edu/nuScore.


BACKGROUND
peter_park@harvard.edu; tolstorukov@gmail.com


BACKGROUND
The site contains user manual, description of the methodology and examples.
Tolstorukov
Michael Y
MY
Harvard-Partners Center for Genetics and Genomics, Brigham and Women's Hospital, Boston, MA 02115, USA. tolstorukov@gmail.com
Choudhary
Vidhu
V
Olson
Wilma K
WK
Zhurkin
Victor B
VB
Park
Peter J
PJ
D000465:Algorithms; D001483:Base Sequence; D002874:Chromosome Mapping / Q000379:methods*; D003198:Computer Simulation; D020407:Internet; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009707:Nucleosomes / Q000235:genetics*; D016415:Sequence Alignment / Q000379:methods; D017422:Sequence Analysis, DNA / Q000379:methods*; D012984:Software; D014584:User-Computer Interface
D009707:Nucleosomes