title: Immunohistochemical analysis of the patterns of p53 and PCNA expression in odontogenic cystic lesions.
abstract: OBJECTIVE
  the role of p53 expression in odontogenic lesions has not been fully determined, but has been associated with cell proliferation. The purpose of this study was to analyze p53 and proliferating cell nuclear antigen (PCNA) expression in 4 different odontogenic lesions.
  
  
  METHODS
  expression of p53 and PCNA was analyzed in radicular and dentigerous cysts, odontogenic keratocysts, and calcifying odontogenic cysts (Gorlin cysts) using monoclonal antibodies for detection of p53 and PCNA.
  
  
  RESULTS
  PCNA expression was significantly greater in the basal layer of radicular cysts and in the suprabasal layer of odontogenic keratocysts; the percentage of p53 positive cells was significantly greater in the suprabasal layer of odontogenic keratocysts.
  
  
  CONCLUSIONS
  The patterns of p53 and PCNA expression in dentigerous and radicular cysts were similar although the two lesions are of different origin. In odontogenic keratocysts and Gorlin cysts, results indicate a different pattern of tumor growth.
authors.affiliation: Faculdade de Odontologia, Universidade do Rio Grande do Sul, Porto Alegre, RS, Brazil. marciago@gmail.com
authors.forename: Márcia Gaiger
authors.initials: MG
authors.lastname: de Oliveira
authors.forename: Isabel da Silva
authors.initials: Ida S
authors.lastname: Lauxen
authors.forename: Anna Cecília Moraes
authors.initials: AC
authors.lastname: Chaves
authors.forename: Pantelis Varvaki
authors.initials: PV
authors.lastname: Rados
authors.forename: Manoel
authors.initials: M
authors.lastname: Sant'Ana Filho
mesh_terms: D006801:Humans; D007150:Immunohistochemistry; D009807:Odontogenic Cysts / Q000276:immunology* / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53