Selection of phage-displayed peptides that bind to a particular ligand-bound antibody.
Phage-displayed peptides that selectively bind to aldolase catalytic antibody 93F3 when bound to a particular 1,3-diketone hapten derivative have been developed using designed selection strategies with libraries containing 7-12 randomized amino acid residues. These phage-displayed peptides discriminated the particular 93F3-diketone complex from ligand-free 93F3 and from 93F3 bound to other 1,3-diketone hapten derivatives. By altering the selection procedures, phage-displayed peptides that bind to antibody 93F3 in the absence of 1,3-diketone hapten derivatives have also been developed. With using these phage-displayed peptides, ligand-bound states of the antibody were distinguished from each other. A docking model of one of the peptides bound to the antibody 93F3-diketone complex was created using a sequential divide-and-conquer peptide docking strategy; the model suggests that the peptide interacts with both the antibody and the ligand through a delicate hydrogen bonding network.
Tanaka
Fujie
F
Department of Molecular Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. ftanaka@scripps.edu
Hu
Yunfeng
Y
Sutton
Jori
J
Asawapornmongkol
Lily
L
Fuller
Roberta
R
Olson
Arthur J
AJ
Barbas
Carlos F
CF
Lerner
Richard A
RA
D000818:Animals; D017151:Antibodies, Catalytic / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000911:Antibodies, Monoclonal / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017104:Bacteriophage M13 / Q000737:chemistry / Q000378:metabolism*; D001666:Binding Sites, Antibody; D002374:Catalase / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007659:Ketones / Q000737:chemistry / Q000378:metabolism; D008024:Ligands; D051379:Mice; D050506:Mutant Chimeric Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D009842:Oligopeptides / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D019151:Peptide Library
D017151:Antibodies, Catalytic; D000911:Antibodies, Monoclonal; D007659:Ketones; D008024:Ligands; D050506:Mutant Chimeric Proteins; D009842:Oligopeptides; D019151:Peptide Library; D002374:Catalase