DNA immobilization/hybridization on plasma-polymerized pyrrole.
The present work describes the fabrication and characterization of the conducting polymer coatings prepared by the continuous wave plasma polymerization and the applications as adhesion layers for studying DNA immobilization/hybridization. The stability of plasma polymerized pyrrole (ppPY) in the aqueous solution was characterized by ellipsometry. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy were used to investigate polymer matrix properties and oligonucleotide/DNA binding interaction. The successful DNA immobilization on ppPY surfaces was found to depend on the macromolecular architecture of plasma polymerized films. The plasma polymers with similar thickness deposited at different input powers showed various comparable immobilization properties. The plasma-polymerized films prepared at the low input power showed a lower sensitivity toward DNA binding than those films deposited at the high input power. This result will be important to study plasma polymerized films as potential DNA biosensors in the future.
Zhang
Zhihong
Z
Henan Provincial Key Laboratory of Surface and Interface Science, Zheng Zhou University of Light Industry, No. 5 Dong Feng Road, Zheng Zhou, China. mainzhh@yahoo.com.cn
Liang
Ping
P
Zheng
Xianjun
X
Peng
Donglai
D
Yan
Fufeng
F
Zhao
Rui
R
Feng
Chuan-Liang
CL
D000268:Adhesiveness; D015374:Biosensing Techniques; D004247:DNA / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D011758:Pyrroles / Q000138:chemical synthesis* / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared
D009841:Oligonucleotides; D011758:Pyrroles; D004247:DNA