[Detection of binding of three aldehyde compounds with DNA using high performance liquid chromatograph].
OBJECTIVE
To detect the binding sites and characteristics of the adduct from the reaction of formaldehyde, acetaldehyde, acrolein with DNA.


METHODS
Formaldehyde, acetaldehyde, acrolein were reacted with four kinds of deoxyribonucleoside monophosphate (dNMP) in buffered solutions with neutral pH. The reaction products were separated by high performance liquid chromatograph (HPLC) and characterized by UV spectroscopy.


RESULTS
The reaction of formaldehyde, acetaldehyde with dG was separated and detected by HPLC. The reaction of acrolein, formate, acetic acid, Mercapturic acid with dG was not separated and detected by HPLC, while the dominant dNMP binding with formaldehyde, acetaldehyde was also determined.


CONCLUSIONS
Formaldehyde, acetaldehyde could bind with dGMP to express genotoxic effects.
Feng
Bin
B
Institute for Industrial Hygiene and Occupational Disease of Shandong Province, Jinan, 250062, China.
Shao
Hua
H
Zhang
Hao
H
D000079:Acetaldehyde / Q000737:chemistry / Q000633:toxicity; D000171:Acrolein / Q000737:chemistry / Q000633:toxicity; D000447:Aldehydes / Q000737:chemistry* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004247:DNA / Q000737:chemistry*; D004249:DNA Damage / Q000187:drug effects; D005557:Formaldehyde / Q000737:chemistry / Q000633:toxicity; D006157:Guanosine Monophosphate / Q000737:chemistry
D000447:Aldehydes; D005557:Formaldehyde; D000171:Acrolein; D006157:Guanosine Monophosphate; D004247:DNA; D000079:Acetaldehyde