A small stem loop element directs internal initiation of the URE2 internal ribosome entry site in Saccharomyces cerevisiae.
Internal initiation of translation is the process of beginning protein synthesis independent of the m(7)G cap structure at the 5'-end of an mRNA molecule. We have previously shown that the URE2 mRNA in the yeast Saccharomyces cerevisiae contains an internal ribosome entry site (IRES) whose activity is suppressed by eukaryotic initiation factor 2A (eIF2A; YGR054W). In this study, the minimal sequence required to efficiently direct internal initiation was determined using a system that abrogates cap-dependent scanning of the 40 S ribosomal subunit in both wild-type and eIF2A knock-out cells. Subsequently, secondary structural elements within the minimal sequence were determined by probing with RNases T1 and V1 and the small molecule diethylpyrocarbonate. It was found that the URE2 minimal IRES comprises a 104 nucleotide A-rich stem loop element encompassing the internal AUG codon. Interestingly, the internal AUG seems to be involved in base-pairing interactions that would theoretically hamper its ability to interact with incoming initiator tRNA molecules. Furthermore, none of the truncations used to identify the minimal IRES element were capable of abrogating the suppressive effect of eIF2A. Our data provide the first insight into the RNA structural requirements of the yeast translational machinery for cap-independent initiation of protein synthesis.
Reineke
Lucas C
LC
Department of Biochemistry, Case Western Reserve University, Cleveland, School of Medicine, Ohio 44106, USA.
Komar
Anton A
AA
Caprara
Mark G
MG
Merrick
William C
WC
D018387:Codon, Initiator / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D004047:Diethyl Pyrocarbonate / Q000737:chemistry; D004722:Endoribonucleases / Q000737:chemistry; D015852:Eukaryotic Initiation Factor-2 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D005979:Glutathione Peroxidase; D009690:Nucleic Acid Conformation; D010442:Peptide Chain Initiation, Translational / Q000502:physiology; D011328:Prions / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017433:Protein Structure, Secondary; D012331:RNA, Fungal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012343:RNA, Transfer / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D006163:Ribonuclease T1 / Q000737:chemistry; D012270:Ribosomes / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D018387:Codon, Initiator; D015852:Eukaryotic Initiation Factor-2; D011328:Prions; D012331:RNA, Fungal; D029701:Saccharomyces cerevisiae Proteins; D012343:RNA, Transfer; D005979:Glutathione Peroxidase; C067020:URE2 protein, S cerevisiae; D004722:Endoribonucleases; C032825:ribonuclease V(1); D006163:Ribonuclease T1; D004047:Diethyl Pyrocarbonate