title: Cutting edge: rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells.
abstract: CC chemokine receptor 10 and its ligand, CCL27, are important components of T cell-mediated cutaneous immunity, but whether they influence lymph node (LN) homing by T cells is unknown. In this study, CCL27 protein was detected in skin-draining LN by Western blotting and ELISA although CCL27 mRNA transcripts were low. CCL27 protein was present at higher levels in skin-draining LN compared with gut-draining LN and spleen. A single topical treatment of mouse skin with the contact sensitizer 2,4-dinitro-1-fluorobenzene (DNFB) resulted in a 13-fold increase in CCL27 protein accumulation in skin-draining LN within 1 h and a 5-fold elevation in CCR10 mRNA (normalized to the T cell marker CD2) within 6 h. DNFB treatment also resulted in rapid depletion of approximately 75% of CCL27 from the epidermis. In summary, we describe a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis.
authors.affiliation: Dermatology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
authors.forename: Victor
authors.initials: V
authors.lastname: Huang
authors.forename: Anke S
authors.initials: AS
authors.lastname: Lonsdorf
authors.forename: Lei
authors.initials: L
authors.lastname: Fang
authors.forename: Takashi
authors.initials: T
authors.lastname: Kakinuma
authors.forename: Vivian C
authors.initials: VC
authors.lastname: Lee
authors.forename: Emily
authors.initials: E
authors.lastname: Cha
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nagao
authors.forename: Marzanna
authors.initials: M
authors.lastname: Zaleska
authors.forename: Waldemar L
authors.initials: WL
authors.lastname: Olszewski
authors.forename: Sam T
authors.initials: ST
authors.lastname: Hwang
mesh_terms: D000328:Adult; D000818:Animals; D015153:Blotting, Western; D054425:Chemokine CCL27 / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D054447:Receptors, CCR10 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
chemical_list: C119538:Ccl27a protein, mouse; C515707:Ccr10 protein, mouse; D054425:Chemokine CCL27; D012333:RNA, Messenger; D054447:Receptors, CCR10; D004139:Dinitrofluorobenzene