FGF21 attenuates lipolysis in human adipocytes - a possible link to improved insulin sensitivity.
Fibroblast growth factor 21 (FGF21) is active in murine adipocytes and has beneficial metabolic effects in animal models of type 2 diabetes mellitus. We assessed whether FGF21 influences lipolysis in human adipocytes and 3T3-L1 cells. FGF21 had no short-time effect (h) while a 3-day incubation with FGF21 attenuated hormone-stimulated lipolysis. FGF21 did not influence the mRNA expression of genes involved in regulating lipolysis, but significantly reduced the expression of the lipid droplet-associated phosphoprotein perilipin without affecting differentiation. Via reduced release of fatty acids into the circulation, the anti-lipolytic effect could be a mechanism through which FGF21 promotes insulin sensitivity in man.
Arner
Peter
P
Department of Medicine, M63 Karolinska Institutet, Karolinska University Hospital, Huddinge, 141 86 Stockholm, Sweden.
Pettersson
Amanda
A
Mitchell
Pamela J
PJ
Dunbar
James D
JD
Kharitonenkov
Alexei
A
Rydén
Mikael
M
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins; D002454:Cell Differentiation / Q000187:drug effects; D005227:Fatty Acids / Q000097:blood / Q000378:metabolism; D005346:Fibroblast Growth Factors / Q000494:pharmacology / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects; D005966:Glucuronidase / Q000378:metabolism*; D006801:Humans; D007333:Insulin Resistance; D000090265:Klotho Proteins; D008066:Lipolysis / Q000187:drug effects / Q000235:genetics; D051379:Mice; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism
D002352:Carrier Proteins; D005227:Fatty Acids; D000070778:Perilipin-1; D010750:Phosphoproteins; C414620:fibroblast growth factor 21; D005346:Fibroblast Growth Factors; D005966:Glucuronidase; D000090265:Klotho Proteins