Uracil-DNA glycosylase (UNG) influences the melting temperature (T(m)) of herpes simplex virus (HSV) hybridization probes.
Prior to PCR amplification, uracil-DNA glycosylase (UNG) can be incorporated to prevent amplicon contamination. Melting temperature (T(m)) analysis of herpes simplex virus (HSV) hybridization probes was used to demonstrate a concentration-dependent decrease in T(m) values when heat stabile or heat labile UNG was added.
Leblanc
Jason J
JJ
Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Center, Halifax, Nova Scotia, Canada.
Pettipas
Janice
J
Campbell
Sarah J
SJ
Davidson
Ross J
RJ
Hatchette
Todd F
TF
D015342:DNA Probes; D004279:DNA, Viral / Q000032:analysis / Q000378:metabolism; D004795:Enzyme Stability; D006561:Herpes Simplex / Q000821:virology; D018259:Herpesvirus 1, Human / Q000235:genetics* / Q000302:isolation & purification; D018258:Herpesvirus 2, Human / Q000235:genetics* / Q000302:isolation & purification; D006358:Hot Temperature; D006801:Humans; D009693:Nucleic Acid Hybridization / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods; D044366:Transition Temperature; D051981:Uracil-DNA Glycosidase / Q000378:metabolism*
D015342:DNA Probes; D004279:DNA, Viral; D051981:Uracil-DNA Glycosidase