[Effect of huangqi and dangshen extraction on angiogenesis in Matrigel implant of mice].
OBJECTIVE
To study the inhibitory effect of huangqi and dangshen extraction (SQ) on angiogenesis induced by b-FGF.


METHODS
Matrigel implant assay was used. Matrigel(500 microL) containing b-FGF and heparin was injected subcutaneously into the abdomens of mice and harvested 5 d later. The amount of hemoglobin and micro-vascular area present in the implant were measured and compared. The mice were given different dosage of SQ (experimental group) or the same volume of glucose (vehicle group) once a day by intraperitoneal injection. Inhibitory experiment started 3 d before Matrigel implant and continued until the end of study.


RESULTS
SQ in lower dosage (< or = 50% V/V) increased hemoglobin content and micro-vascular area in Matrigel implant while SQ in higher dosage (> or = 60%, V/V) reduced hemoglobin content and micro-vascular area in Matrigel implant. The effect of enhance ment and inhibition was in a limited concentration-effect manner.


CONCLUSIONS
SQ in different dosage has different effects on angiogenesis. We should use different dosage in different purpose.
Bai
Chang-qing
CQ
Department of Respiratory Diseases, Affiliated Hospital, Academy of Military Medical Sciences, Beijing 100071, China. mlp1604@sina.com
Song
Ying-fang
YF
Jian
Wen
W
Wang
De-tang
DT
Ma
Cun-bao
CB
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000009:adverse effects / Q000494:pharmacology; D001808:Blood Vessels / Q000187:drug effects* / Q000502:physiology; D029745:Codonopsis / Q000737:chemistry*; D003094:Collagen / Q000009:adverse effects / Q000494:pharmacology*; D004195:Disease Models, Animal; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D007797:Laminin / Q000009:adverse effects / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009369:Neoplasms / Q000139:chemically induced* / Q000503:physiopathology; D009389:Neovascularization, Pathologic / Q000139:chemically induced*; D010936:Plant Extracts / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011509:Proteoglycans / Q000009:adverse effects / Q000494:pharmacology*
D020533:Angiogenesis Inhibitors; D001672:Biocompatible Materials; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; D007797:Laminin; D010936:Plant Extracts; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen