One-electron oxidation of condensed DNA toroids: injection-site dependent charge (radical cation) mobility.
DNA condensates were formed by treating linear pUC19 plasmids ligated to an AQ-containing oligomer with spermidine. The condensates are toroid-shaped objects having a radius of 70 to 100 nm. Irradiation of the condensates with UV light (absorbed by the anthraquinone) causes the one-electron oxidation of the DNA and concomitant reaction at GG steps of the oligomer. Analysis of the distance dependence of the reaction at guanine in these condensates reveals a dependency on the position of the AQ. This observation is attributed to a reduction in the rate for trapping of the radical cation in the relatively dehydrated interior of the condensate.
Das
Prolay
P
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
Schuster
Gary B
GB
D000880:Anthraquinones / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism*; D004249:DNA Damage / Q000528:radiation effects; D004583:Electrons; D010084:Oxidation-Reduction; D010957:Plasmids / Q000378:metabolism; D013095:Spermidine / Q000737:chemistry; D014466:Ultraviolet Rays
D000880:Anthraquinones; D004247:DNA; D013095:Spermidine