title: Effects of pH shock on the secretion system in Streptomyces coelicolor A3(2).
abstract: Effects of pH shock on the secretion system of S. coelicolor A3(2) have been investigated at a transcriptional level by using DNA microarrays. Actinorhodin secretion was observed to be highly enhanced when an acidic-pH shock was applied to surface grown cultures of S. coelicolor A3(2). In this culture, a gene of actVA-orf1 encoding a putative efflux pump or transporter protein for actinorhodin was strongly upregulated. A major number of efflux pumps for other metabolites and a major number of secretion proteins for protein secretion were also observed to be upregulated with pH shock. The secretion of actinorhodin was observed to be remarkably enhanced in liquid culture as well.
authors.affiliation: Department of Chemical and Biomolecular Engineering (The Brain Korea 21 Program), Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
authors.forename: Yoon Jung
authors.initials: YJ
authors.lastname: Kim
authors.forename: Jae Yang
authors.initials: JY
authors.lastname: Song
authors.forename: Soon-Kwang
authors.initials: SK
authors.lastname: Hong
authors.forename: Colin P
authors.initials: CP
authors.lastname: Smith
authors.forename: Yong Keun
authors.initials: YK
authors.lastname: Chang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D019149:Bioreactors; D015964:Gene Expression Regulation, Bacterial; D006863:Hydrogen-Ion Concentration; D020411:Oligonucleotide Array Sequence Analysis; D021381:Protein Transport; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins