title: Hydrophilic interaction and reversed-phase ultra-performance liquid chromatography TOF-MS for metabonomic analysis of Zucker rat urine.
abstract: Hydrophilic interaction chromatography (HILIC) provides a complementary technique to RP methods for the retention of polar analytes for LC-MS-based metabonomic studies. Combining the advantages of both RP and HILIC separations with the efficient and rapid separations obtained using sub-2 mum particles via the recently introduced ultra-performance LC (UPLC) enables increased coverage of the metabolites present in biological samples to be achieved. Here an HILIC-UPLC-MS method was developed to provide metabolite profiles for urine samples obtained from male Zucker rats. The resulting data were compared with results obtained for the same samples by RP-UPLC-MS and demonstrated the complementary nature of the two separations with both methods enabling discrimination between the different sample types. Interestingly sample type differentiation was based on different markers.
authors.affiliation: Department of Drug Metabolism and Pharmacokinetics, AstraZeneca, Cheshire, UK.
authors.forename: Helen G
authors.initials: HG
authors.lastname: Gika
authors.forename: Georgios A
authors.initials: GA
authors.lastname: Theodoridis
authors.forename: Ian D
authors.initials: ID
authors.lastname: Wilson
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D008297:Male; D009765:Obesity / Q000652:urine; D044367:Phase Transition; D051381:Rats; D011924:Rats, Zucker / Q000652:urine; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D016482:Urinalysis / Q000379:methods*; D014556:Urine / Q000737:chemistry