Effects of defensin and lactoferrin on functional activity of endothelial cells in vitro.
We studied the effects of antibacterial peptides and proteins (defensins and lactoferrins) on functional activity of endothelial cells in vitro: proliferative activity and adhesion of human endothelial ECV-304 cells to the matrix were evaluated, alpha-Defensin (NP-2) from rabbit neutrophils, total alpha-defensin (HNP 1-3) from human neutrophils, and lactoferrins from porcine neutrophils and human milk were studied. Defensins stimulated and lactoferrin in doses of 1-10 microg/ml inhibited proliferation and adhesion of endothelial cell. The stimulatory effect of defensins on proliferation and adhesion was reproduced in fibroblast culture. Lactoferrins did not modify proliferation of fibroblasts, but suppressed their adhesion. These data suggest that antibiotic proteins and peptides are prospective objects for the creation of drugs regulating angiogenesis.
Krylov
A V
AV
Institute of Experimental Medicine, Russian Academy of Medical Sciences, St. Petersburg.
Kisseleva
E P
EP
Aleshina
G M
GM
Shamova
O V
OV
Kokryakov
V N
VN
D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D042783:Endothelial Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007781:Lactoferrin / Q000494:pharmacology*; D011817:Rabbits; D023084:alpha-Defensins / Q000494:pharmacology*
D023084:alpha-Defensins; D007781:Lactoferrin