title: A technicolour approach to the connectome.
abstract: A central aim of neuroscience is to map neural circuits, in order to learn how they account for mental activities and behaviours and how alterations in them lead to neurological and psychiatric disorders. However, the methods that are currently available for visualizing circuits have severe limitations that make it extremely difficult to extract precise wiring diagrams from histological images. Here we review recent advances in this area, along with some of the opportunities that these advances present and the obstacles that remain.
authors.affiliation: Department of Molecular and Cellular Biology and Center for Brain Science, Harvard University, Cambridge, Massachusetts 02138, USA. jeff@mcb.harvard.edu
authors.forename: Jeff W
authors.initials: JW
authors.lastname: Lichtman
authors.forename: Jean
authors.initials: J
authors.lastname: Livet
authors.forename: Joshua R
authors.initials: JR
authors.lastname: Sanes
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000502:physiology* / Q000648:ultrastructure; D001931:Brain Mapping / Q000379:methods*; D004396:Coloring Agents; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics; D008854:Microscopy, Electron; D009434:Neural Pathways / Q000502:physiology / Q000648:ultrastructure; D009474:Neurons / Q000502:physiology; D009488:Neurosciences / Q000379:methods* / Q000639:trends*; D013194:Staining and Labeling; D019076:Transgenes
chemical_list: D004396:Coloring Agents; D008164:Luminescent Proteins