title: The UL31 to UL35 gene sequences of Duck enteritis virus correspond to their homologs in herpes simplex virus 1.
abstract: Five ORFs in the genome of Duck enteritis virus (DEV) corresponding to UL31, UL32, UL33, UL34, and UL35 genes of Herpes simplex virus 1 (HSV-1) were amplified by a modified "targeted gene walking" PCR, cloned, and sequenced. UL33, UL34, and UL35 genes were oriented from the left to the right of genome, while UL31 and UL32 had an opposite orientation. A comparison of deduced amino acid sequences of the DEV ORFs with their alphaherpesvirus homologs showed well-conserved regions except for the UL34 and UL35 genes. Phylogenetic analysis revealed that DEV was closer to the genus Mardivirus than to any other genus of the subfamily Alphaherpesvirinae. Based on this evidence, we proposed to assign DEV to the subfamily Alphaherpesvirinae.
authors.affiliation: Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
authors.forename: R
authors.initials: R
authors.lastname: An
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.forename: Z
authors.initials: Z
authors.lastname: Han
authors.forename: Y
authors.initials: Y
authors.lastname: Shao
authors.forename: S
authors.initials: S
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Kong
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D004372:Ducks; D018259:Herpesvirus 1, Human / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D025922:Mardivirus / Q000737:chemistry / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D014764:Viral Proteins