Effect of O6-methylguanine on the stability of G-quadruplex DNA.
The effects of substitution of O6-methylguanine on the structure and stability of a human telomere quadruplex was studied by circular dichroism, thermal denaturation, analytical ultracentrifugation, and molecular dynamics simulations. The results show that, while quadruplex structures can form containing the modified base, they are much less stable than the normal unmodified structure. The extent of destabilization is critically dependent on the exact position of the modified base within the quadruplex structure.
Mekmaysy
Chongkham S
CS
James Graham Brown Cancer Center and University of Louisville, 529 South Jackson Street, Louisville, Kentucky 40202, USA.
Petraccone
Luigi
L
Garbett
Nichola C
NC
Ragazzon
Patricia A
PA
Gray
Robert
R
Trent
John O
JO
Chaires
Jonathan B
JB
D002942:Circular Dichroism; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry*; D019175:DNA Methylation; D054856:G-Quadruplexes; D006147:Guanine / Q000031:analogs & derivatives* / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry
D009841:Oligonucleotides; D006147:Guanine; D004247:DNA; C008449:O-(6)-methylguanine