Enhancement of permeability in endothelial cells for the development of an antithrombogenic bioartificial hemofilter.
For the development of an antithrombogenic bioartificial hemofilter, in which the inner surface of hollow fibers is lined by endothelial cells, it is essential to increase the permeability of the cells in order to achieve a sufficient ultrafiltrate. We tried to increase it by using an actin microfilament polymerization inhibitor, cytochalasin B (CyB). Fifty microg/mL CyB was added for 2 h to the culture medium of confluent rat glomerular endothelial cells (RGEC) and human umbilical vein endothelial cells (HUVEC). Under the 130 mmHg hydrostatic pressure, the CyB-treated group produced significantly more ultrafiltration than the non-treated control group and this increase was maintained for at least 7 days. Horseradish peroxidase (HRP) permeability acutely and reversibly increased in the CyB-treated group compared with the non-treated control group. Scanning electron microscopy revealed a larger average diameter and increased number of fenestrae on the CyB-treated endothelial cells, compared with the non-treated cells. This phenomenon also lasted for at least 7 days. The platelet adherence test showed that CyB did not deteriorate the antithrombogenic property of endothelial cells. These results indicate that CyB is potentially applicable for the enhancement of endothelial cell permeability in an antithrombogenic bioartificial hemofilter.
Vu
Duc M
DM
Division of Nephrology and Metabolism, Department of Medicine, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
Yokoyama
Tun A
TA
Sawada
Kaichiro
K
Inagaki
Miho
M
Kanai
Genta
G
Lu
Jianxin
J
Kakuta
Takatoshi
T
Adler
Stephen
S
Nangaku
Masaomi
M
Saito
Akira
A
D000818:Animals; D002460:Cell Line; D003571:Cytochalasin B / Q000494:pharmacology; D042783:Endothelial Cells / Q000187:drug effects* / Q000648:ultrastructure; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006440:Hemofiltration / Q000379:methods*; D006735:Horseradish Peroxidase / Q000378:metabolism; D006801:Humans; D006874:Hydrostatic Pressure; D008855:Microscopy, Electron, Scanning; D010539:Permeability / Q000187:drug effects*; D051381:Rats
D004791:Enzyme Inhibitors; D003571:Cytochalasin B; D006735:Horseradish Peroxidase