Fingerprint of Hedyotis diffusa Willd. by HPLC-MS.
A HPLC-MS fingerprint method has been developed based on the consistent chromatographic features of the major chemical constituents among 10 batches of Hedyotis diffusa Willd. Chromatographic separation was conducted on a Hypersil-Keystone Hypurity C(18) column using methanol:water:acetic acid as the mobile phase. Major compounds, including oleanolic acid, ursolic acid and ferulic acid, were analysed by HPLC-MS. Their analysis was ascertained by comparison with data derived from the standard compounds. The HPLC-MS fingerprint was successfully applied to analyse and differentiate samples from different geographical origins, or processing methods. H. diffusa was well distinguished from Hedyotis chrysotricha by HPLC-MS. Therefore the establishment of fingerprint of H. diffusa is critical in assessing and controlling its overall quality.
Yang
Ting
T
School of Biosciences and Technologies, Central South University, Changsha, Hunan 410013, People's Republic of China.
Yang
Yi-Hua
YH
Yang
Ju-Yun
JY
Chen
Ben-Mei
BM
Duan
Ju-Ping
JP
Yu
Shu-Yi
SY
Ouyang
Hong-Tao
HT
Cheng
Jun-Ping
JP
Chen
Yu-Xiang
YX
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis*; D032064:Hedyotis / Q000737:chemistry*; D013058:Mass Spectrometry; D015203:Reproducibility of Results
D004365:Drugs, Chinese Herbal