title: Evaluation of c4d deposition and circulating antibody in small bowel transplantation.
abstract: Antibody-mediated rejection (AMR) consensus criteria are defined in kidney and heart transplantation by histological changes, circulating donor-specific antibody (DSA), and C4d deposition in affected tissue. AMR consensus criteria are not yet identified in small bowel transplantation (SBTx). We investigated those three criteria in 12 children undergoing SBTx, including one retransplantation and four combined liver-SBTx (SBTx), with a follow-up of 12 days to 2 years. All biopsies (91) were evaluated with a standardized grading scheme for acute rejection (AR), vascular lesions and C4d expression. Sera were obtained at day 0 and during the follow-up. C4d was expressed in 37% of biopsies with or without AR, but in 50% of biopsies with severe vascular lesions. In addition, vascular lesions were always associated with AR and a poor outcome. All children with AR (grade 2 or 3) observed before the third month died or lost the graft. DSA were never found in any studied sera. We found no evidence that C4d deposition was of any clinical relevance to the outcome of SBTx. However, the grading of vascular lesions may constitute a useful marker to identify AR that is potentially resistant to standard treatment, and for which an alternative therapy should be considered.
authors.affiliation: Necker Enfants Malades-Pathology, Tumorothèque, Paris, France. natacha.patey-mariaud@nck.aphp.fr
authors.forename: N Patey-Mariaud
authors.initials: NP
authors.lastname: de Serre
authors.forename: D
authors.initials: D
authors.lastname: Canioni
authors.forename: F
authors.initials: F
authors.lastname: Lacaille
authors.forename: C
authors.initials: C
authors.lastname: Talbotec
authors.forename: D
authors.initials: D
authors.lastname: Dion
authors.forename: N
authors.initials: N
authors.lastname: Brousse
authors.forename: O
authors.initials: O
authors.lastname: Goulet
mesh_terms: D000293:Adolescent; D000906:Antibodies / Q000097:blood*; D002648:Child; D002675:Child, Preschool; D003181:Complement C4 / Q000276:immunology*; D005260:Female; D006084:Graft Rejection / Q000276:immunology*; D006801:Humans; D007223:Infant; D007421:Intestine, Small / Q000276:immunology* / Q000637:transplantation*; D008297:Male; D016377:Organ Transplantation; D011446:Prospective Studies
chemical_list: D000906:Antibodies; D003181:Complement C4