Electrochemical regulation of the end-product profile in Propionibacterium freudenreichii ET-3 with an endogenous mediator.
The end-product profile of the glucose fermentation by Propionibacterium freudenreichii ET-3 changed on an electrochemical treatment, in which the culture vessel was filled with a carbon felt anode. Acetate and propionate were produced as final end products in a molar ratio of 2:3 without any electrochemical treatments at the point of the consumption of lactate as an intermediate of the glucose fermentation. The ratio was changed to 1:1 at the point of the lactate consumption by the electrochemical incubation at an electrode potential of 0.4 V versus Ag|AgCl for 100 h. During further electrochemical incubation, propionate was oxidized to acetate as a final end-product in the microbe-containing anode chamber. 1,4-Dihydroxy-2-naphthoic acid produced by P. freudenreichii ET-3 itself would receive electrons from the metabolic pathway and serve as an electron transfer mediator from the microbial cells to the electrode.
Wang
Yung-Fu
YF
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Masuda
Masaki
M
Tsujimura
Seiya
S
Kano
Kenji
K
D019342:Acetic Acid / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods*; D004563:Electrochemistry; D005947:Glucose / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D009284:Naphthols / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*; D011424:Propionibacterium / Q000737:chemistry* / Q000378:metabolism*
D009284:Naphthols; D011422:Propionates; C024023:1,4-dihydroxy-2-naphthoic acid; D019344:Lactic Acid; D005947:Glucose; D019342:Acetic Acid