Dendroflorin retards the senescence of MRC-5 cells.
The influence of dendroflorin, a potentially active compound extracted from Dendrobium nobile, on cell growth and cell cycles was assessed in a human embryo lung fibroblastic MRC-5 cell line. An MTT assay indicated that dendroflorin benefits cell proliferation. Additionally, we used flow cytometry to examine the cell cycles in senescent MRC-5 cells after treatment with dendroflorin, discovering that dendroflorin could trigger cells in the G1 phase to enter the S phase. Further examination of intracellular ROS contents using DCFH-DA suggested that dendroflorin helps ROS degradation, partly elucidating the mechanism of its effects. All of the above results suggest that dendroflorin is a potential candidate with anti-senescence activity.
Jin
Jinghua
J
Protein Science Key Laboratory of the Ministry of Education, Department of Biological Sciences and Biotechnology, School of Medicine, Tsinghua University, Beijing, People's Republic of China.
Liang
Yu
Y
Xie
Hong
H
Zhang
Xue
X
Yao
Xinsheng
X
Wang
Zhao
Z
D002453:Cell Cycle / Q000187:drug effects; D002460:Cell Line; D016922:Cellular Senescence / Q000187:drug effects*; D031667:Dendrobium / Q000737:chemistry; D005434:Flow Cytometry; D016166:Free Radical Scavengers / Q000494:pharmacology*; D016193:G1 Phase / Q000187:drug effects; D006801:Humans; D018384:Oxidative Stress / Q000187:drug effects; D017382:Reactive Oxygen Species / Q000378:metabolism; D016196:S Phase / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles
D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D013778:Tetrazolium Salts; D013844:Thiazoles; C022616:thiazolyl blue