title: Two new steroidal compounds from starfish Asterias amurensis Lutken.
abstract: Two new sulfated steroidal compounds (1 and 2), along with three known steroidal saponins (3, 4, and 5) were isolated from the starfish Asterias amurensis Lutken. The structures of new compounds were elucidated as 3beta-O-sulfated-cholest-5-ene-7alpha-ol (1) and (E) 25-O-beta-d-xylopyranosyl-26, 27-dinor-24(S)-methyl-22-ene-15alpha-O-sulfated-5alpha-cholest-3beta,6alpha-ol (2) by extensive NMR experiments and chemical evidence. Their effects on UMR106 cell proliferation were screened by MTT method. The results indicated that compounds 2 and 2a (0.01-100 microM) significantly promoted the osteoblastic proliferation. The initial structure-activity relationship analysis suggests that the sugar moiety is the necessary group for the activity.
authors.affiliation: School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, China. liuhongwei60@yahoo.com.cn
authors.forename: Hong-Wei
authors.initials: HW
authors.lastname: Liu
authors.forename: Jian-Kuan
authors.initials: JK
authors.lastname: Li
authors.forename: Da-Wei
authors.initials: DW
authors.lastname: Zhang
authors.forename: Jing-Chao
authors.initials: JC
authors.lastname: Zhang
authors.forename: Nai-Li
authors.initials: NL
authors.lastname: Wang
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Cai
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Yao
mesh_terms: D000818:Animals; D047271:Asterias / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D008968:Molecular Conformation; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: D012503:Saponins; D013256:Steroids