[Dermatophytes classification by PCR.].
Many authors attempt to solve problems in fungal taxonomy using techniques based in molecular biology. We find reports based in different molecular techniques studying genomic, nuclear or mitochondrial DNA that led the authors to establish several specific or generic differences in this group of fungi. But mostly of them are long laboratory procedures. At present study we study a 1450 bp fragment of mtDNA encoding the ND4, ATP6 and SsrDNA and adjacent SsrRNA 3' termini sequence. We use three primer pairs previously designed. The other primer pair amplify the adjacent region of the SSrRNA gene is used as modified by Li et al. Our purpose is to find differences at the species level.
Pereiro Ferreirós
M
M
Departamento de Dermatología, Complejo Hospitalario Universitario, Facultad de Medicina, Universidad de Santiago de Compostela, Spain. manuelpe@usc.es.
Flórez Menéndez
A
A
Toribio Pérez
J
J