title: CD47 deficiency protects mice from lipopolysaccharide-induced acute lung injury and Escherichia coli pneumonia.
abstract: CD47 modulates neutrophil transmigration toward the sites of infection or injury. Mice lacking CD47 are susceptible to Escherichia coli (E. coli) peritonitis. However, less is known concerning the role of CD47 in the development of acute lung inflammation and injury. In this study, we show that mice lacking CD47 are protected from LPS-induced acute lung injury and E. coli pneumonia with a significant reduction in pulmonary edema, lung vascular permeability, and bacteremia. Reconstitution of CD47(+/-) mice with CD47(-/-) neutrophils significantly reduced lung edema and neutrophil infiltration, thus demonstrating that CD47(+) neutrophils are required for the development of lung injury from E. coli pneumonia. Importantly, CD47-deficient mice with E. coli pneumonia had an improved survival rate. Taken together, deficiency of CD47 protects mice from LPS-induced acute lung injury and E. coli pneumonia. Targeting CD47 may be a novel pathway for treatment of acute lung injury.
authors.affiliation: Cardiovascular Research Institute, University of California, San Francisco, CA 94143, USA. suxiao@yahoo.com
authors.forename: Xiao
authors.initials: X
authors.lastname: Su
authors.forename: Mette
authors.initials: M
authors.lastname: Johansen
authors.forename: Mark R
authors.initials: MR
authors.lastname: Looney
authors.forename: Eric J
authors.initials: EJ
authors.lastname: Brown
authors.forename: Michael A
authors.initials: MA
authors.lastname: Matthay
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry / Q000166:cytology / Q000276:immunology; D051928:CD47 Antigen / Q000276:immunology / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D004797:Enzyme-Linked Immunosorbent Assay; D004927:Escherichia coli Infections / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D008070:Lipopolysaccharides / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000276:immunology* / Q000378:metabolism; D018410:Pneumonia, Bacterial / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000276:immunology / Q000378:metabolism* / Q000473:pathology
chemical_list: D051928:CD47 Antigen; D016207:Cytokines; D008070:Lipopolysaccharides