Unusual fatty acid isomers of triacylglycerols and polar lipids in female limpet gonads of Cellana grata.
To investigate the occurrence of positional isomers of unsaturated fatty acids (FA) in triacylglycerols (TAG) and polar lipids (PL) in the female gonads of a dominant limpet, Cellana grata, fatty acid methyl esters (FAME) were fractionated according to the degree of their unsaturation by argentation thin-layer chromatography. Their structures were elucidated by gas chromatography-mass spectrometry of a combination of their FAME and picolinyl esters. A total of 125 different FA ranging from 12 to 24 carbon atoms were identified, and 105 unsaturated FA, including 34 nonmethylene-interrupted FA as minor components, were recognized. Although the major FA 16:0 and 18:1n-7, which accounted for more than 20 and 10%, respectively, of total FA, were present in TAG and PL, higher amounts of 20:4n-6 and 20:5n-3 were present in PL. Unsaturated FA consisting of more than five different positional isomers were 17:1, 19:1, 18:2, 19:2, 20:2, 21:2, 22:2, 20:3, and 22:3, most of which were present in TAG. Furthermore, a larger variety of nonmethylene-interrupted FA was found in TAG than in PL. This limpet gonad had a diverse assortment of unsaturated FA that comprised a wide range of unusual positional isomers with one to five double bonds.
Kawashima
Hideki
H
Bioscience Laboratory, Miyako College, Iwate Prefectural University, Miyako 027-0039, Japan. ajoe@iwate-pu.ac.jp
Ohnishi
Masao
M
Ogawa
Satoshi
S
Matsui
Kenji
K
D000818:Animals; D002384:Catalysis; D005227:Fatty Acids / Q000737:chemistry*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006066:Gonads / Q000737:chemistry*; D007536:Isomerism; D008055:Lipids / Q000737:chemistry*; D008974:Mollusca; D014280:Triglycerides / Q000737:chemistry*
D005227:Fatty Acids; D008055:Lipids; D014280:Triglycerides