Optical clearing in transcutaneous Raman spectroscopy of murine cortical bone tissue.
The effect of optical clearing with glycerol on the Raman spectra of bone tissue acquired transcutaneously on right and left tibiae from four mice is studied. Multiple transcutaneous measurements are obtained from each limb; glycerol is then applied as an optical clearing agent, and additional transcutaneous measurements are taken. Glycerol reduces the noise in the raw spectra (p=0.0037) and significantly improves the cross-correlation between the recovered bone factor and the exposed bone measurement in a low signal-to-noise region of the bone spectra (p=0.0245).
Schulmerich
Matthew V
MV
University of Michigan, Department of Chemistry, Ann Arbor, Michigan 48109, USA.
Cole
Jacqueline H
JH
Dooley
Kathryn A
KA
Morris
Michael D
MD
Kreider
Jaclynn M
JM
Goldstein
Steven A
SA
D000818:Animals; D005990:Glycerol / Q000008:administration & dosage*; D066298:In Vitro Techniques; D051379:Mice; D012031:Refractometry; D013059:Spectrum Analysis, Raman / Q000379:methods*; D013977:Tibia / Q000187:drug effects* / Q000502:physiology*
D005990:Glycerol