Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene-oligonucleotide conjugate.
Novel biological activities of a fullerene-oligonucleotide conjugate (FONC) were examined in the present report. A new FONC containing a complementary sequence to a specific region of the beta-actin cDNA was synthesized. We found that the FONC at a low concentration (10(-6)M) could specifically inhibit PCR-amplification of the beta-actin cDNA by inhibiting the activities of both Taq DNA polymerase and the cDNA template. This inhibition appeared to be mediated by reactive oxygen species (ROS). The FONC also exhibited the ability of antagonizing the enzymatic activity of an engineered nuclease, exonuclease I (Exo I). Our data provided some valuable evidences on the prospective use of FONC in the antisense strategy for gene silencing.
Yang
Xinlin
X
School of Life Science and Technology, Beijing Institute of Technology, 5 Zhongguancun South Street, Beijing 100081, China. xlyang@bit.edu.cn
Meng
Xianmei
X
Li
Bo
B
Chen
Zhe
Z
Zhao
Dongxu
D
Tan
Xin
X
Yu
Qun
Q
D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology; D005090:Exodeoxyribonucleases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D037741:Fullerenes / Q000737:chemistry / Q000494:pharmacology*; D005784:Gene Amplification / Q000187:drug effects*; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D008954:Models, Biological; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D009841:Oligonucleotides / Q000737:chemistry / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D017382:Reactive Oxygen Species / Q000378:metabolism
D004791:Enzyme Inhibitors; D037741:Fullerenes; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D017382:Reactive Oxygen Species; D004247:DNA; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I