Effects of nestin-antisense treatment in mice on memory-related performance.
Nestin is a neuronal stem cell marker. It has been reported that newly generated adult neurons play a role in murine memory. The purpose of this study was to determine whether nestin itself plays a role in memory function in adult animals. Mice were infused intraventricularly with nestin-selective, morpholino-modified antisense oligodeoxynucleotides for 6 days, after which their ability to perform a new memory-related, food-search task was assessed. Such treatment was found to reduce the training effect on food-search test performance. Immunohistochemical staining revealed that nestin-positive cells within the hippocampal dentate gyrus and subventricular zone were attenuated in these animals. These data support a role for nestin in adult memory and learning.
Kurokawa
Mamoru
M
Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan. black@nagasaki-u.ac.jp
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D002452:Cell Count / Q000379:methods; D007381:Intermediate Filament Proteins / Q000378:metabolism*; D008124:Locomotion / Q000187:drug effects; D008297:Male; D008568:Memory / Q000187:drug effects*; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D064231:Nestin; D016376:Oligonucleotides, Antisense / Q000494:pharmacology*; D011597:Psychomotor Performance / Q000187:drug effects; D013997:Time Factors
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D016376:Oligonucleotides, Antisense