title: Inter-kingdom conservation of mechanism of nonsense-mediated mRNA decay.
abstract: Nonsense-mediated mRNA decay (NMD) is a quality control system that degrades mRNAs containing premature termination codons. Although NMD is well characterized in yeast and mammals, plant NMD is poorly understood. We have undertaken the functional dissection of NMD pathways in plants. Using an approach that allows rapid identification of plant NMD trans factors, we demonstrated that two plant NMD pathways coexist, one eliminates mRNAs with long 3'UTRs, whereas a distinct pathway degrades mRNAs harbouring 3'UTR-located introns. We showed that UPF1, UPF2 and SMG-7 are involved in both plant NMD pathways, whereas Mago and Y14 are required only for intron-based NMD. The molecular mechanism of long 3'UTR-based plant NMD resembled yeast NMD, whereas the intron-based NMD was similar to mammalian NMD, suggesting that both pathways are evolutionarily conserved. Interestingly, the SMG-7 NMD component is targeted by NMD, suggesting that plant NMD is autoregulated. We propose that a complex, autoregulated NMD mechanism operated in stem eukaryotes, and that despite aspect of the mechanism being simplified in different lineages, feedback regulation was retained in all kingdoms.
authors.affiliation: Agricultural Biotechnology Center, Gödöllo, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Kerényi
authors.forename: Zsuzsanna
authors.initials: Z
authors.lastname: Mérai
authors.forename: László
authors.initials: L
authors.lastname: Hiripi
authors.forename: Anna
authors.initials: A
authors.lastname: Benkovics
authors.forename: Péter
authors.initials: P
authors.lastname: Gyula
authors.forename: Christophe
authors.initials: C
authors.lastname: Lacomme
authors.forename: Endre
authors.initials: E
authors.lastname: Barta
authors.forename: Ferenc
authors.initials: F
authors.lastname: Nagy
authors.forename: Dániel
authors.initials: D
authors.lastname: Silhavy
mesh_terms: D020413:3' Untranslated Regions / Q000378:metabolism*; D000595:Amino Acid Sequence; D018389:Codon, Nonsense / Q000378:metabolism*; D006706:Homeostasis; D007438:Introns; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D020871:RNA Stability; D018749:RNA, Plant / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D018389:Codon, Nonsense; D010940:Plant Proteins; D018749:RNA, Plant