Hyper-phosphorylation of alpha-enolase in hypertrophied left ventricle of spontaneously hypertensive rat.
Cardiac hypertrophy is one of the main target organ damages of essential hypertension and predicts a poor prognosis of the disease. The molecules involved in this event, especially their posttranslational modifications, remain largely unknown to date. With a combination of phosphoprotein column enrichment and two-dimensional gel electrophoresis separation, here we compared the profiling of enriched phosphoproteins from the left ventricle (LV) of spontaneously hypertensive rats (SHR) to that of age-matched Wistar Kyoto rats. As a result, 19 differential proteins were found in the hypertrophied LV of SHR. Among them, we focused on a glycolysis enzyme alpha-enolase, of which the hyper-phosphorylation was shown in the hypertrophied LV but not in non-hypertrophied atrium and right ventricle of SHR. Furthermore, the alpha-enolase hyper-phosphorylation was accompanied by decreased enzymatic activity. The further investigation based on these results would provide new clues to understand the pathological process of cardiac hypertrophy in SHR.
Jin
Xian
X
The Department of Geriatrics, Ren-Ji Hospital, Shanghai Jiao-Tong University School of Medicine, Shanghai 200001, China.
Wang
Li-Shun
LS
Xia
Li
L
Zheng
Ying
Y
Meng
Chao
C
Yu
Yun
Y
Chen
Guo-Qiang
GQ
Fang
Ning-Yuan
NY
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D006973:Hypertension / Q000150:complications*; D017379:Hypertrophy, Left Ventricular / Q000201:enzymology* / Q000209:etiology; D010750:Phosphoproteins / Q000032:analysis / Q000378:metabolism*; D010751:Phosphopyruvate Hydratase / Q000032:analysis / Q000378:metabolism*; D010766:Phosphorylation; D040901:Proteomics; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY
D010750:Phosphoproteins; D010751:Phosphopyruvate Hydratase