title: Dynamics of immune cell recruitment during West Nile encephalitis and identification of a new CD19+B220-BST-2+ leukocyte population.
abstract: West Nile virus (WNV) is an emerging neurotropic flavivirus. We investigated the dynamics of immune cell recruitment in peripheral tissues and in the CNS during WNV encephalitis in an immunocompetent mouse model. In the periphery, immune cell expansion can successfully limit viremia and lymphoid tissue infection. However, viral clearance in the periphery is too late to prevent viral invasion of the CNS. In the CNS, innate immune cells, including microglia/macrophages, NK cells, and plasmacytoid dendritic cells, greatly expand as the virus invades the brain, whereas B and T cells are recruited after viral invasion, and fail to control the spread of the virus. Thus, the onset of WNV encephalitis was correlated both with CNS viral infection and with a large local increase of innate immune cells. Interestingly, we identify a new immune cell type: CD19(+)B220(-) BST-2(+), which we name G8-ICs. These cells appear during peripheral infection and enter the CNS. G8-ICs express high levels of MHC class II, stain for viral Ag, and are localized in the paracortical zone of lymph nodes, strongly suggesting they are previously unidentified APCs that appear in response to viral infection.
authors.affiliation: Neuro-Immunologie Virale, Institut Pasteur, Paris, France.
authors.forename: Anne-Claire
authors.initials: AC
authors.lastname: Bréhin
authors.forename: Juliette
authors.initials: J
authors.lastname: Mouriès
authors.forename: Marie-Pascale
authors.initials: MP
authors.lastname: Frenkiel
authors.forename: Gilles
authors.initials: G
authors.lastname: Dadaglio
authors.forename: Philippe
authors.initials: P
authors.lastname: Desprès
authors.forename: Monique
authors.initials: M
authors.lastname: Lafon
authors.forename: Thérèse
authors.initials: T
authors.lastname: Couderc
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism*; D018941:Antigens, CD19 / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology / Q000821:virology; D019070:Cell Lineage; D002490:Central Nervous System / Q000166:cytology / Q000276:immunology / Q000821:virology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D058851:GPI-Linked Proteins; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D007694:Killer Cells, Natural / Q000276:immunology; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D007962:Leukocytes / Q000276:immunology / Q000378:metabolism*; D008198:Lymph Nodes / Q000166:cytology / Q000276:immunology; D008264:Macrophages / Q000276:immunology; D008562:Membrane Glycoproteins / Q000378:metabolism*; D051379:Mice; D017628:Microglia / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology / Q000821:virology; D014901:West Nile Fever / Q000276:immunology*; D000074010:Bone Marrow Stromal Antigen 2
chemical_list: D015703:Antigens, CD; D018941:Antigens, CD19; D058851:GPI-Linked Proteins; D000949:Histocompatibility Antigens Class II; D017493:Leukocyte Common Antigens; D008562:Membrane Glycoproteins; C094572:BST2 protein, human; D000074010:Bone Marrow Stromal Antigen 2