title: Identification of the cis-acting elements involved in regulation of xylanase III gene expression in Trichoderma reesei PC-3-7.
abstract: The xylanase III gene (xyn3) from the filamentous fungus Trichoderma reesei PC-3-7 is only induced by cellulose, its derivatives and L-sorbose, but not by xylan. In this study, we defined three cis-acting elements within the xyn3 upstream region by using detailed deletion and mutation analysis. In addition to the Xyr1/ACEII-binding motif (5'-GGCTAA-3'), the analogous motifs 5'-GGCTAT-3' and 5'-GGCAAA-3' presented as an inverted repeat spaced by 16-bp internal sequences were identified as essential elements for xyn3 expression. Electrophoretic mobility shift assay using heterologously expressed Xyr1 demonstrates that all the identified cis-acting elements are able to interact with Xyr1. Furthermore, no xyn3 transcripts were formed in the xyr1-knockout strain upon induction by sophorose and L-sorbose. These results indicate that xyn3 expression is transcriptionally regulated by Xyr1, and suggest that the 5'-GGCTAT-3' and 5'-GGCAAA-3' motifs play roles in Xyr1-mediated cellulase and xylanase gene expression in T. reesei.
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, 1603-1, Kamitomioka, Nagaoka, Niigata 940-2188, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Furukawa
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Shida
authors.forename: Naoki
authors.initials: N
authors.lastname: Kitagami
authors.forename: Yuuki
authors.initials: Y
authors.lastname: Ota
authors.forename: Masahiro
authors.initials: M
authors.lastname: Adachi
authors.forename: Shiho
authors.initials: S
authors.lastname: Nakagawa
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Shimada
authors.forename: Masashi
authors.initials: M
authors.lastname: Kato
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Okada
authors.forename: Wataru
authors.initials: W
authors.lastname: Ogasawara
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Morikawa
mesh_terms: D001483:Base Sequence; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D017930:Genes, Reporter; D020218:Response Elements; D017384:Sequence Deletion; D014158:Transcription, Genetic; D014242:Trichoderma / Q000201:enzymology* / Q000235:genetics* / Q000378:metabolism; D014995:Xylosidases / Q000235:genetics* / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; D014995:Xylosidases