Casitone-dependent transcriptional regulation of the prtP and prtM genes in the natural isolate Lactobacillus paracasei subsp. paracasei.
The prtP-prtM intergenic region of Lactobacillus paracasei subsp. paracasei BGHN 14 was cloned and sequenced. The nucleotide sequence of the prtP-prtM intergenic region in BGHN 14, containing divergently orientated P(prtP) and P(prtP) promoters, was shorter by 35 bp in comparison with that in lactococci. The nucleotide sequence involved in casitone-dependent transcriptional regulation of the lactococcal prt genes was not found in the BGHN14. The activity of P(prtM) in L. lactis NZ9000 was very low and insignificantly changed in the presence of casitone, whereas P(prtP) was completely inactive. When L. casei ATCC393(T) was used as host, both P(prtP) and P(prtM) were active and strongly regulated by casitone. The results strongly indicate that the mechanisms of the casitone-dependent regulation of the prt genes in BGHN14 and lactococci are different.
Pastar
I
I
Institute of Molecular Genetics and Genetic Engineering, P.O. Box 23, 11010 Belgrade, Serbia. lab6@eunet.yu
Begović
J
J
Lozo
J
J
Topisirović
L
L
Golić
N
N
D019677:Artificial Gene Fusion; D001426:Bacterial Proteins / Q000378:metabolism*; D001483:Base Sequence; D002364:Caseins / Q000378:metabolism; D003001:Cloning, Molecular; D004269:DNA, Bacterial / Q000235:genetics; D021901:DNA, Intergenic; D015964:Gene Expression Regulation, Bacterial; D017930:Genes, Reporter; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D007778:Lactobacillus / Q000235:genetics*; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA; D024363:Transcription Initiation Site; D014158:Transcription, Genetic
D001426:Bacterial Proteins; D002364:Caseins; D004269:DNA, Bacterial; D021901:DNA, Intergenic; C475729:casein hydrolysate; D005966:Glucuronidase