Application of restriction fragment length polymorphism analysis to simple and rapid genotyping of bovine viral diarrhea virus strains isolated in Japan.
The E2 regions of 177 bovine viral diarrhea virus (BVDV) strains isolated in Japan between 1957 and 2006 were analyzed for genotyping. The strains were classified into 8 genotypes (1a, 1b, 1c, 1d, 1e, 1f, So and 2a) based on the phylogenetic analysis. The restriction fragment length polymorphism (RFLP) analysis of the RT-PCR products using 6 selected enzymes (Apo I, Mly I, BstAP I, Pvu II, Ear I, EcoR V) disclosed the cutting patterns classified into 11 groups (I-XI), each of that consisted of strains belonging to a single genotype. Namely, groups-I and -II were composed by genotype-1a strains, groups-III and -IV by 1b strains, and groups-V and -VI by 1c strains. Other groups-VII, -VIII, -IX, -X and -XI comprised genotypes-1d, -1e, -1f, -So and -2a strains, respectively. The results suggest that the RFLP analysis can simply and rapidly differentiate the 8 genotypes of BVDV strains.
Seki
Yoshihisa
Y
Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa-mura, Iwate, Japan. yoshihisa-seki@pref.iwate.jp
Seimiya
Yukio M
YM
Motokawa
Masato
M
Yaegashi
Gakuji
G
Nagai
Makoto
M
Hayashi
Michiko
M
D000818:Animals; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification* / Q000235:genetics*; D005838:Genotype; D007564:Japan; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length