Green procedure with a green solvent for fats and oils' determination. Microwave-integrated Soxhlet using limonene followed by microwave Clevenger distillation.
Here is described a green and original alternative procedure for fats and oils' determination in oleaginous seeds. Extractions were carried out using a by-product of the citrus industry as extraction solvent, namely d-limonene, instead of hazardous petroleum solvents such as n-hexane. The described method is achieved in two steps using microwave energy: at first, extractions are attained using microwave-integrated Soxhlet, followed by the elimination of the solvent from the medium using a microwave Clevenger distillation in the second step. Oils extracted from olive seeds were compared with both conventional Soxhlet and microwave-integrated Soxhlet extraction procedures performed with n-hexane in terms of qualitative and quantitative determination. No significant difference was obtained between each extract allowing us to conclude that the proposed method is effective and valuable.
Virot
Matthieu
M
UMR 408 Sécurité et Qualité des Produits d'Origine Végétale, INRA, Université d'Avignon et des Pays du Vaucluse, 84000 Avignon, France.
Tomao
Valérie
V
Ginies
Christian
C
Visinoni
Franco
F
Chemat
Farid
F
D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D053138:Cyclohexenes / Q000737:chemistry*; D000077222:Limonene; D008872:Microwaves; D015394:Molecular Structure; D000069463:Olive Oil; D010938:Plant Oils / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012997:Solvents; D013729:Terpenes / Q000737:chemistry*
D053138:Cyclohexenes; D000069463:Olive Oil; D010938:Plant Oils; D012997:Solvents; D013729:Terpenes; D000077222:Limonene