[Studies of genetic diversity among 10 species of Ilex based on RAPD and AFLPs].
The techniques of random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphisms (AFLPs) were used to amplify the genomic DNA of 10 species of Ilex, so as to reveal the polymorphism in different species of Ilex. In RAPD analysis, a total of 301 lines were amplified with 11 kinds of primers selected from 100 kinds of 10 bp random primers, and the polymorphism was 98.63%. In AFLPs analysis,a huge diversity was showed by 3 primers combinations carried out on these species. Both RAPD and AFLPs analysis were clustered by UPGMA and the results showed that the genetic distances were the closest between I. purpurea and I. vomitoria Ait, I. Litseaefolia and I. zhejiangensis, I. hylonoma var. glabra and I. triflora var. kanehirai.
Qian
Yong Sheng
YS
Key Laboratory of Hangzhou City for Biochemistry and Molecular Biology, Hangzhou Normal University, Hangzhou 310018, China.
Wang
Hui Zhong
HZ
Shi
Nong Nong
NN
Zhao
Yan
Y
Li
Nian Lin
NL
Hu
Zhong
Z
D054458:Amplified Fragment Length Polymorphism Analysis; D014644:Genetic Variation; D030017:Ilex / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique