title: CE-LIF method for the separation of anthracyclines: application to protein binding analysis in plasma using ultrafiltration.
abstract: Anthracyclines are chemotherapeutic drugs that are widely used in the treatment of cancers such as lung and ovarian cancers. The simultaneous determination of the anthracyclines, daunorubicin, doxorubicin and epirubicin, was achieved using CE coupled to LIF, with an excitation and emission wavelength of 488 and 560 nm, respectively. Using a borate buffer (105 mM, pH 9.0) and 30% MeOH, a stable and reproducible separation of the three anthracyclines was obtained. The method developed was shown to be capable of monitoring the therapeutic concentrations (50-50 000 ng/mL) of anthracyclines. LODs of 10 ng/mL, calculated at an S/N = 3, were achieved. Using the CE method developed, the in vitro protein binding to plasma was measured by ultrafiltration, and from this investigation the estimated protein binding was determined to be in the range of 77-94%.
authors.affiliation: R. N. Adams Institute of Bioanalytical Chemistry, University of Kansas, KS, USA.
authors.forename: Gillian
authors.initials: G
authors.lastname: Whitaker
authors.forename: Amy
authors.initials: A
authors.lastname: Lillquist
authors.forename: Stephanie A
authors.initials: SA
authors.lastname: Pasas
authors.forename: Robert
authors.initials: R
authors.lastname: O'Connor
authors.forename: Fiona
authors.initials: F
authors.lastname: Regan
authors.forename: Craig E
authors.initials: CE
authors.lastname: Lunte
authors.forename: Malcolm R
authors.initials: MR
authors.lastname: Smyth
mesh_terms: D018943:Anthracyclines / Q000032:analysis* / Q000097:blood*; D000903:Antibiotics, Antineoplastic / Q000032:analysis* / Q000097:blood*; D000970:Antineoplastic Agents / Q000032:analysis* / Q000097:blood*; D001881:Borates / Q000737:chemistry; D003630:Daunorubicin / Q000032:analysis; D004317:Doxorubicin / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D015251:Epirubicin / Q000032:analysis; D006801:Humans; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D011485:Protein Binding; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D018943:Anthracyclines; D000903:Antibiotics, Antineoplastic; D000970:Antineoplastic Agents; D001881:Borates; D015251:Epirubicin; D004317:Doxorubicin; D000432:Methanol; D003630:Daunorubicin