Neurotrophic effects of leptin on cerebellar Purkinje but not granule neurons in vitro.
As recent evidence has revealed a pro-survival role for the anti-obesity hormone leptin in the nervous system, we investigated the generality of this finding on cerebellar Purkinje and granule neurons in vitro. We found that whilst leptin promoted cerebellar Purkinje neuron survival, it had no affect on cerebellar granule cells. In addition, we discovered that leptin promoted both the outgrowth of neurites from cerebellar Purkinje neurons and increased the complexity of the neurite arbor. Thus, leptin has different effects on two neighbouring populations of neurons within the cerebellum implying specificity of its actions in the central nervous system.
Oldreive
Ceri E
CE
School of Biology, University of St. Andrews, St. Andrews, Fife KY16 9TS, UK.
Harvey
Jenni
J
Doherty
Gayle H
GH
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002525:Cerebellar Cortex / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D003712:Dendrites / Q000187:drug effects / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D020738:Leptin / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D009414:Nerve Growth Factors / Q000378:metabolism* / Q000494:pharmacology; D016501:Neurites / Q000187:drug effects / Q000378:metabolism; D011689:Purkinje Cells / Q000187:drug effects / Q000378:metabolism*; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D054411:Receptors, Leptin / Q000819:agonists / Q000235:genetics / Q000378:metabolism
D020738:Leptin; D009414:Nerve Growth Factors; D012333:RNA, Messenger; D054411:Receptors, Leptin