title: [Construction and targeting study of eukaryotic expression vector modulated by a macrophage-specific promoter].
abstract: OBJECTIVE
  To construct a macrophage-specific eukaryotic expression vector and to investigate its expressing specificity.
  
  
  METHODS
  Macrophage-specific promoter was synthesized using PCR, and substituted the CMV promoter of eukaryotic expression vector pEGFP-N1 to construct a recombinant vector (pSP-GFP), which were cotransfected with pERFP-N1 vector into different cell lines. The green fluorescent protein (GFP) and red fluorescent protein (RFP) was observed by fluorescence microscopy, and the target specificity of macrophage-specific promoter was judged by comparison of expressed level of GFP and RFP in various cell lines.
  
  
  RESULTS
  The macrophage-specific eukaryotic expression vector was successfully constructed, which showed strong activity and specificity only in macrophage cells.
  
  
  CONCLUSIONS
  The constructed pSP-GFP vector was macrophage-specific, which are potential to targeted gene therapy of intracellular bacterial infection.
authors.affiliation: Faculty of Laboratory Medicine, Chongqing Medical University, the Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing 400016, China. wenyangan0105@yahoo.com.cn
authors.forename: Yang-an
authors.initials: YA
authors.lastname: Wen
authors.forename: Jin-ying
authors.initials: JY
authors.lastname: Guo
authors.forename: Xiao-lin
authors.initials: XL
authors.lastname: Yu
authors.forename: Dong
authors.initials: D
authors.lastname: Cen
authors.forename: Jin-bo
authors.initials: JB
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhi-guang
authors.initials: ZG
authors.lastname: Tu
mesh_terms: D004742:Enhancer Elements, Genetic; D056890:Eukaryota / Q000235:genetics*; D015316:Genetic Therapy; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000502:physiology*; D008297:Male; D011401:Promoter Regions, Genetic / Q000235:genetics* / Q000502:physiology; D019098:Telomerase / Q000378:metabolism; D014162:Transfection; D019444:Vaccines, DNA / Q000008:administration & dosage; D037521:Virulence Factors / Q000235:genetics
chemical_list: D019444:Vaccines, DNA; D037521:Virulence Factors; D049452:Green Fluorescent Proteins; D019098:Telomerase