Parameters for DNA vaccination using adaptive constant-current electroporation in mouse and pig models.
Enhancing the expression of DNA vaccines requires that specific conditions of delivery are optimized. We describe experiments using adaptive constant-current electroporation (EP) in mice and pigs examining parameters such as target muscle, delay between plasmid delivery and onset of EP pulses and DNA vaccine formulation; our studies show that concentrated formulations result in better expression and immunogenicity. Furthermore, various conditions of EP that limit the amount of muscle damage were measured. The results of these studies will help to advance the success of DNA vaccines in animals into success in human clinical trials.
Draghia-Akli
Ruxandra
R
VGX Pharmaceuticals, 2700 Research Forest Drive, Suite 180, The Woodlands, TX 77381, United States. rdraghia@vgxp.com
Khan
Amir S
AS
Brown
Patricia A
PA
Pope
Melissa A
MA
Wu
Ling
L
Hirao
Lauren
L
Weiner
David B
DB
D000469:Alkaline Phosphatase / Q000097:blood / Q000235:genetics / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000097:blood*; D018274:Electroporation; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000097:blood / Q000235:genetics*; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000502:physiology; D012867:Skin / Q000378:metabolism*; D013552:Swine; D014611:Vaccination; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology / Q000378:metabolism
D000906:Antibodies; D019444:Vaccines, DNA; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D000469:Alkaline Phosphatase