Computational comparison of the stacking interactions between the aromatic amino acids and the natural or (cationic) methylated nucleobases.
The strongest gas-phase MP2/6-31G*(0.25) stacking energies between the aromatic amino acids and the natural or methylated nucleobases were considered. The potential energy surfaces of dimers were searched as a function of the vertical separation, angle of rotation and horizontal displacement between monomers stacked according to their centers of mass. Our calculations reveal that the stacking interactions of adducts for a given nucleobase are dependent on the methylation site (by up to 20 kJ mol(-1)), where the relative magnitudes of the interactions are determined by the dipole moments of the adducts and the proton affinities of nucleobase methylation sites. Nevertheless, the differences in the (gas-phase) stacking of methylated adducts are small compared with the differences between the stacking of the corresponding natural and methylated nucleobases. Indeed, methylation increases the stacking energy by up to 40 kJ mol(-1) (or 135%). Although immersing the dimers in different solvents decreases the gas-phase stacking energies with an increase in the polarity of the environment, base methylation still has a significant effect on the nucleobase stacking ability in solvents with large dipole moments, and, perhaps more importantly, environments that mimic enzyme active sites. Our results shed light on the workings of DNA repairs enzymes that selectively remove a wide variety of alkylated nucleobases over the natural bases.
Rutledge
Lesley R
LR
Department of Chemistry and Biochemistry, University of Lethbridge, Lethbridge, AB, Canada.
Durst
Holly F
HF
Wetmore
Stacey D
SD
D000596:Amino Acids / Q000737:chemistry*; D002412:Cations; D008745:Methylation; D009696:Nucleic Acids / Q000737:chemistry*
D000596:Amino Acids; D002412:Cations; D009696:Nucleic Acids