title: Redox activity ofHydrodictyon reticulatum plasmalemma vesicles.
abstract: Plasmalemma vesicles with preserved redox activity were prepared from nets ofHydrodictyon reticulatum. Since the walls are mechanically very resistant, a combination of partial cell-wall enzyme digestion and ultrasonic homogenization had to be used for the disruption of cells. To isolate the plasma-membrane-enriched microsomal fraction separation in an aqueous two-phase polymer system was found to be most suitable. The right-side-out vesicles reduced added hexacyanoferrate(III) by electrons supplied either by a transmembrane flow or from external NADH.
authors.affiliation: Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague.
authors.forename: J
authors.initials: J
authors.lastname: Matousková
authors.forename: L
authors.initials: L
authors.lastname: Nespůrková
authors.forename: R
authors.initials: R
authors.lastname: Rybová
authors.forename: K
authors.initials: K
authors.lastname: Janácek