title: Evaluation of a multicolor, single-tube technique to enumerate lymphocyte subpopulations.
abstract: To evaluate the fully automated FACSCanto software, we compared lymphocyte subpopulation counts obtained using three-color FACSCalibur-CELLQuest and six-color FACSCanto-FACSCanto software techniques. High correlations were observed between data obtained with these techniques. Our study indicated that FACSCanto clinical software is accurate and sensitive in single-platform lymphocyte immunophenotyping.
authors.affiliation: Cellular Therapy and Cryobiology, Regenerative Medicine Department, Centre of Transfusion Medicine, IRCCS Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy. labcitofl@policlinico.mi.it
authors.forename: F
authors.initials: F
authors.lastname: Colombo
authors.forename: A
authors.initials: A
authors.lastname: Cattaneo
authors.forename: R
authors.initials: R
authors.lastname: Lopa
authors.forename: P
authors.initials: P
authors.lastname: Portararo
authors.forename: P
authors.initials: P
authors.lastname: Rebulla
authors.forename: L
authors.initials: L
authors.lastname: Porretti
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D016131:Lymphocyte Subsets / Q000276:immunology*; D012680:Sensitivity and Specificity; D012984:Software
chemical_list: D015703:Antigens, CD