Age-related changes of calbindin D-28k-immunoreactive neurons in the myenteric plexus of gerbil duodenum.
We examined the age-related changes of calbindin D-28k (CB)-immunoreactive neurons and overall populations of neurons in the myenteric plexus of gerbil duodenum using whole mount preparations and immunohistochemistry. The circumference of duodenum increased age-dependently. CB-immunoreactive neurons were observed in all groups, and most of them had the Dogiel type II morphology. The fully developed cobweb-like structures were observed in the myenteric plexus of duodenum at postnatal month (PM) 3 to 24. Although the highest numbers of CB-immunoreactive neurons and overall population were observed in PM 1.5, it is related with significant increase of the size of circumference between PM 1.5 to PM 3. CB-immunoreactive neurons were slightly decreased with age between PM 3 to PM 24. We have also found that whole numbers of myenteric neurons were also significantly decreased in PM 24 group. These results suggest that loss of overall numbers of myenteric neurons and CB-immunoreactive neurons may be related with age-related neurodegeneration and functional loss of duodenum in the gerbil.
Choi
Jung Hoon
JH
Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, South Korea.
Lee
Choong Hyun
CH
Chung
Dae Won
DW
Hwang
In Koo
IK
Won
Moo Ho
MH
Seong
Je Kyung
JK
Yoon
Yeo Sung
YS
Lee
In Se
IS
D000375:Aging; D000818:Animals; D064026:Calbindins; D004386:Duodenum / Q000294:innervation*; D005786:Gene Expression Regulation; D005849:Gerbillinae / Q000378:metabolism*; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology*; D009474:Neurons / Q000378:metabolism*; D064030:S100 Calcium Binding Protein G / Q000235:genetics / Q000378:metabolism*
D064026:Calbindins; D064030:S100 Calcium Binding Protein G