Life stage- dependent bioconcentration of a nonylphenol isomer in Daphnia magna.
Bioaccumulation is an important aspect for the fate and effects of xenobiotics in the environment. In this study we used a radiolabeled nonylphenol isomer to investigate the bioconcentration in Daphnia magna at different ages. Apart from the total radioactivity we measured the metabolism of p353-NP in D. magna, to calculate the amount of p353-NP compared to total radioactivity found within the daphnids. Bioconcentration factors, based on wet weight, calculated from the rate constants for total radioactivity in neonates and adults were 4271 kg/l and 760 kg/l respectively, leading to a 5.6 deviance in bioconcentration. This deviance was even more pronounced, nearly one order of magnitude, for the p353-NP concentration with bioconcentration factors of 302 kg/l for neonates and 31 kg/l for adults. We were able to describe the bioconcentration for all daphnids by a weight-dependent one- compartment model. These results pointed out that it is not possible to compare bioconcentration experiments conducted with different substances and different sized daphnids. Additionally it was shown that it is not possible to describe the bioconcentration by measuring the total radioactivity. Metabolism of nonylphenol occurs at a very fast rate and bioconcentration is not triggered by the partition between two phases, but by metabolism. Discrimination between the two mechanisms was achieved using radiolabeled substances and a pseudo two-compartment model to describe metabolism and elimination by two rate constants which afterwards can be compared between different substances.
Preuss
Thomas G
TG
Institute of Environmental Research (Biology V), Aachen University, Worringerweg 1, D-52066 Aachen, Germany. thomas.preuss@bio5.rwth-aachen.de
Telscher
Markus
M
Ratte
Hans Toni
HT
D000818:Animals; D003621:Daphnia / Q000737:chemistry* / Q000378:metabolism / Q000502:physiology; D004463:Ecology / Q000379:methods; D007536:Isomerism; D007553:Isotope Labeling; D008018:Life Cycle Stages; D008657:Metabolic Clearance Rate; D008954:Models, Biological; D010636:Phenols / Q000032:analysis* / Q000378:metabolism; D011868:Radioisotopes; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000378:metabolism; D015262:Xenobiotics / Q000032:analysis* / Q000378:metabolism
D010636:Phenols; D011868:Radioisotopes; D014874:Water Pollutants, Chemical; D015262:Xenobiotics; C025256:nonylphenol