title: Assessment of bone formation capacity using in vivo transplantation assays: procedure and tissue analysis.
abstract: In vivo assessment of bone formation (osteogenesis) potential by isolated cells is an important method for analysis of cells and factors control ling bone formation. Currently, cell implantation mixed with hydroxyapa-tite/tricalcium phosphate in an open system (subcutaneous implantation) in immunodeficient mice is the standard method for in vivo assessment of bone formation capacity of a particular cell type. The method is easy to perform and provides reproducible results. Assessment of the donor origin of tissue formation is possible, especially in the case of human-to-mouse transplanta tion, by employing human specific antibodies or in situ hybridization using human specific Alu-repeat probes. Recently, several methods have been developed to quantitate the newly formed bone using histomorphometric methods or using non-invasive imaging methods. This chapter describes the use of in vivo transplantation methods in testing bone formationpotential of human mesenchymal stem cells.
authors.affiliation: Department of Endocrinology, KMEB Laboratory, University Hospital of Odense, Denmark.
authors.forename: Basem M
authors.initials: BM
authors.lastname: Abdallah
authors.forename: Nicholas
authors.initials: N
authors.lastname: Ditzel
authors.forename: Moustapha
authors.initials: M
authors.lastname: Kassem
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000502:physiology; D001842:Bone and Bones / Q000166:cytology / Q000502:physiology; D002130:Calcium Phosphates / Q000737:chemistry / Q000378:metabolism; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D051379:Mice; D016688:Mice, Inbred NOD; D016513:Mice, SCID; D010012:Osteogenesis; D019736:Prostheses and Implants; D023822:Tissue Engineering / Q000379:methods
chemical_list: D002130:Calcium Phosphates; D017886:Durapatite; C018392:tricalcium phosphate