Silver enhancement of Nanogold particles during freeze substitution for electron microscopy.
Recent advances in rapid freezing and fixation by freeze substitution have allowed structural cell biologists to apply these reliable modes of sample preparation to a wide range of specimens and scientific problems. Progress in electron tomography has produced cellular images with resolution approaching 4 nm in 3D, but our ability to localize macromolecules in these well-fixed, well-resolved samples has remained limited. When light fixation and low temperature embedding are employed with appropriate resins, immuno-localizations can recognize antigens at a section's surface, but labelling is therefore confined, not throughout the section's depth. Small, electron-dense markers, like Nanogold(R), will often enter a living cell, serving as reliable tracers for endocytic activity, but these markers are usually too small to be visible in the context of a cell. We have developed a method for the silver enhancement of Nanogold particles that works during freeze substitution in organic solvents at low temperature. Here, we describe the development of this method, based on in vitro tests of reagents and conditions. We then show results from application of the method to an in vivo system, using Nanogold to track the internalization of immunoglobulin by neonatal murine intestinal epithelium, a specific example of receptor-mediated membrane traffic.
Morphew
M
M
Laboratory for 3D Electron Microscopy of Cells, Department of M.C.D. Biology, University of Colorado, Boulder, CO 80309-0347, USA. mary.morphew@colorado.edu
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W
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Bjorkman
P J
PJ
McIntosh
J Richard
JR
D000818:Animals; D000831:Animals, Newborn; D004847:Epithelial Cells / Q000378:metabolism; D017110:Freeze Substitution / Q000379:methods*; D006046:Gold / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D007074:Immunoglobulin G / Q000737:chemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D008854:Microscopy, Electron / Q000379:methods*; D051381:Rats; D012834:Silver
D007074:Immunoglobulin G; D012834:Silver; D006046:Gold