title: Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense.
abstract: Detailed structures of the active polysaccharides extracted from the leaf and stem cell walls and mucilage of Dendrobium huoshanense are determined by using various techniques, including chromatographic, spectroscopic, chemical, and enzymatic methods. The mucilage polysaccharide exhibits specific functions in activating murine splenocytes to produce several cytokines including IFN-gamma, IL-10, IL-6, and IL-1alpha, as well as hematopoietic growth factors GM-CSF and G-CSF. However, the deacetylated mucilage obtained from an alkaline treatment fails to induce cytokine production. The structure and bioactivity of mucilage components are validated by further fractionation. This is the first study that provides clear evidence for the structure and activity relationship of the polysaccharide in D. huoshanense.
authors.affiliation: The Genomics Research Center, Academia Sinica, Taipei 115, Taiwan.
authors.forename: Yves S-Y
authors.initials: YS
authors.lastname: Hsieh
authors.forename: Cheng
authors.initials: C
authors.lastname: Chien
authors.forename: Sylvian K-S
authors.initials: SK
authors.lastname: Liao
authors.forename: Shih-Fen
authors.initials: SF
authors.lastname: Liao
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Hung
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Yang
authors.forename: Chih-Chien
authors.initials: CC
authors.lastname: Lin
authors.forename: Ting-Jen Rachel
authors.initials: TJ
authors.lastname: Cheng
authors.forename: Chia-Chuan
authors.initials: CC
authors.lastname: Chang
authors.forename: Jim-Min
authors.initials: JM
authors.lastname: Fang
authors.forename: Chi-Huey
authors.initials: CH
authors.lastname: Wong
mesh_terms: D000818:Animals; D001089:Arabinose / Q000032:analysis; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D002473:Cell Wall / Q000737:chemistry; D005591:Chemical Fractionation; D016207:Cytokines / Q000096:biosynthesis; D031667:Dendrobium / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D051379:Mice; D008969:Molecular Sequence Data; D010368:Pectins / Q000032:analysis; D010429:Pentoses / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D014990:Xylans / Q000032:analysis
chemical_list: D016207:Cytokines; D010429:Pentoses; D011134:Polysaccharides; D014990:Xylans; D010368:Pectins; D001089:Arabinose; C003181:polygalacturonic acid