[Wenyang Huoxue Recipe up-regulates the expression of angiopoietin-1 mRNA in rats with chronic aristolochic acid nephropathy].
OBJECTIVE
To investigate the effects of Wenyang Huoxue Recipe (WRHXR), a compound traditional Chinese herbal medicine for warming yang and promoting blood flow, on the expression of angiopoietin mRNA in rats with chronic aristolochic acid nephropathy induced by Caulis Aristolochia Manshuriensis (CAM) decoction, and to explore the protection mechanism of WYHXR against kidney damage.


METHODS
Twenty-eight male SD rats were randomly divided into normal control group, CAM group and WYHXR-treated group. Rats in the normal control group (n=8) and CAM group (n=10) were intragastrically administered with normal saline 10 ml/(kg.d) or CAM decoction 10 ml/(kg.d) respectively. Rats in the WYHXR-treated group (n=10) were intragastrically administered with WYHXR 30 g/(kg.d) and CAM decoction 10 ml/(kg.d). The expressions of Ang-l and Ang-2 mRNAs were detected by real-time reverse transcription polymerase chain reaction (RT-PCR) after 20-week treatment.


RESULTS
Compared with the normal control group, the expression of Ang-l mRNA was significantly decreased, and the expression of Ang-2 mRNA was significantly increased in the CAM group (P<0.01). Compared with the CAM group, the expression of Ang-l mRNA was increased in the WYHXR-treated group (P<0.01). The expression of Ang-2 mRNA had no significant difference between the CAM group and the WYHXR-treated group (P>0.05). Renal pathology showed that renal damage in WYHXR-treated group was significantly reduced as compared with the CAM group.


CONCLUSIONS
WYHXR can up-regulate the expression of Ang-l mRNA, which may be its action mechanism in protecting the kidneys.
Wang
Hong-Ting
HT
Department of Nephrology, Putuo District Center Hospital, Shanghai 200062, China.
Wang
Yun-Man
YM
Liang
Yong-Ping
YP
Liu
Yu-Jun
YJ
Peng
Wen
W
D042683:Angiopoietin-1 / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D030022:Aristolochia / Q000737:chemistry; D034341:Aristolochic Acids / Q000633:toxicity*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D007668:Kidney / Q000378:metabolism; D007683:Kidney Tubular Necrosis, Acute / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D008517:Phytotherapy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015854:Up-Regulation / Q000187:drug effects
D042683:Angiopoietin-1; D034341:Aristolochic Acids; D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; C000228:aristolochic acid I