title: Determination of ciprofloxacin with functionalized cadmium sulfide nanoparticles as a fluorescence probe.
abstract: A novel assay of ciprofloxacin with a sensitivity at the microgram level is proposed based on the measurement of enhanced fluorescence intensity signals resulting from the interaction of functionalized nano-CdS with ciprofloxacin. The CdS nanoparticles was synthesized by thioacetamide (TAA) and cadmium nitrate (Cd(NO(3))(2)) in the alkaline solution. At pH 7.4, the fluorescence signals of functionalized nano-CdS were greatly enhanced by ciprofloxacin with the increase concentration of ciprofloxacin. Linear relationship can be established between the enhanced fluorescence intensity and ciprofloxacin concentration in the range of (1.25-8.75)x10(-4) mg mL(-1) ((3.77-26.4) x 10(-4)mmol L(-1)) or (8.75-1200) x 10(-4)mg mL(-1) ((26.4-3625) x 10(-4) mmol L(-1)). The limit of detection is 7.64 x 10(-6) mg mL(-1) (2.31 x 10(-5)mmol L(-1)). Based on this, a new direct quantitative determination method for ciprofloxacin in human serum samples without separation of foreign substances was established. The contents of ciprofloxacin in human serum samples were determined with recovery of 95-105% and relative standard deviation (R.S.D.) of 1.5-2.5%. This method was proved to be very sensitive, rapid, simple and tolerance of most interfering substances.
authors.affiliation: Key Laboratory of Drug Quality Control and Pharmacovigilance, Ministry of Education, Nanjing 210009, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Yan
authors.forename: Wei-Qing
authors.initials: WQ
authors.lastname: Cheng
mesh_terms: D002021:Buffers; D019187:Cadmium Compounds / Q000032:analysis / Q000737:chemistry*; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D002939:Ciprofloxacin / Q000032:analysis / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007477:Ions; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D013440:Sulfides / Q000032:analysis / Q000737:chemistry*; D013696:Temperature
chemical_list: D002021:Buffers; D019187:Cadmium Compounds; D003102:Colloids; D005456:Fluorescent Dyes; D007477:Ions; D013440:Sulfides; C034939:cadmium sulfide; D002939:Ciprofloxacin