Heterotrophic denitrification at extremely high salt and pH by haloalkaliphilic Gammaproteobacteria from hypersaline soda lakes.
In this paper we describe denitrification at extremely high salt and pH in sediments from hypersaline alkaline soda lakes and soda soils. Experiments with sediment slurries demonstrated the presence of acetate-utilizing denitrifying populations active at in situ conditions. Anaerobic enrichment cultures at pH 10 and 4 M total Na(+) with acetate as electron donor and nitrate, nitrite and N(2)O as electron acceptors resulted in the dominance of Gammaproteobacteria belonging to the genus Halomonas. Both mixed and pure culture studies identified nitrite and N(2)O reduction as rate-limiting steps in the denitrification process at extremely haloalkaline conditions.
Shapovalova
A A
AA
Winogradsky Institute of Microbiology, Russian Academy of Sciences, Prospect 60-let Octyabrya 7/2, 117811 Moscow, Russia.
Khijniak
T V
TV
Tourova
T P
TP
Muyzer
G
G
Sorokin
D Y
DY
D000085:Acetates / Q000378:metabolism; D000693:Anaerobiosis; D020563:Gammaproteobacteria / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D019015:Geologic Sediments / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D007630:Kenya; D008969:Molecular Sequence Data; D008986:Mongolia; D009566:Nitrates / Q000378:metabolism*; D009573:Nitrites / Q000378:metabolism; D009589:Nitrogen Oxides / Q000378:metabolism; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012964:Sodium / Q000378:metabolism; D014871:Water Microbiology
D000085:Acetates; D007220:Industrial Waste; D009566:Nitrates; D009573:Nitrites; D009589:Nitrogen Oxides; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012964:Sodium