title: Effect of nitrogen amendment on respiration and respiratory quotient (RQ) in three hydrocarbon contaminated soils of different type.
abstract: Three soil types (sandy gravel, silty clay and sandy loam) from sites historically contaminated with total petroleum hydrocarbon (TPH) were amended with NH(4)NO(3) at concentrations ranging from 16 to 2133 mg/kg soil(dry weight). Microbial activity was measured as O(2) consumption and CO(2) production in order to assess nitrogen limitation. Although activity was stimulated in all three soils under NH(4)NO(3) amendment (after 72 h), the level of nitrogen required was soil specific. For the sandy gravel and silty clay soils, O(2) consumption and CO(2) production both showed enhanced microbial activity when amended with 16 mg/kg soil(dry weight) NH(4)NO(3), whereas, these two parameters gave differing results for the sandy loam soil. Specifically, CO(2) production and O(2) consumption were stimulated with 66 mg/kg and 133 mg/kg soil(dry weight) of NH(4)NO(3) respectively. In addition, respiratory quotient kinetic analysis suggested different decomposition processes occurring in this soil under different NH(4)NO(3) amendment concentrations.
authors.affiliation: Environmental Reclamation Services Ltd., Westerhill Road, Bishopbriggs, Glasgow G64 2QH, Scotland, UK. tom@ersremediation.com
authors.forename: Thomas
authors.initials: T
authors.lastname: Aspray
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Gluszek
authors.forename: David
authors.initials: D
authors.lastname: Carvalho
mesh_terms: D001673:Biodegradation, Environmental; D002245:Carbon Dioxide / Q000032:analysis; D009566:Nitrates / Q000737:chemistry*; D010100:Oxygen / Q000032:analysis*; D010578:Petroleum / Q000032:analysis*; D012987:Soil / Q000032:analysis / Q000592:standards; D012988:Soil Microbiology / Q000592:standards; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D009566:Nitrates; D010578:Petroleum; D012987:Soil; D012989:Soil Pollutants; D002245:Carbon Dioxide; D010100:Oxygen; C006568:ammonium nitrate