title: Regulation of in situ to invasive breast carcinoma transition.
abstract: The transition of ductal carcinoma in situ (DCIS) to invasive carcinoma is a poorly understood key event in breast tumor progression. Here, we analyzed the role of myoepithelial cells and fibroblasts in the progression of in situ carcinomas using a model of human DCIS and primary breast tumors. Progression to invasion was promoted by fibroblasts and inhibited by normal myoepithelial cells. Molecular profiles of isolated luminal epithelial and myoepithelial cells identified an intricate interaction network involving TGFbeta, Hedgehog, cell adhesion, and p63 required for myoepithelial cell differentiation, the elimination of which resulted in loss of myoepithelial cells and progression to invasion.
authors.affiliation: Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA 02115, USA.
authors.forename: Min
authors.initials: M
authors.lastname: Hu
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.forename: Danielle K
authors.initials: DK
authors.lastname: Carroll
authors.forename: Stanislawa
authors.initials: S
authors.lastname: Weremowicz
authors.forename: Haiyan
authors.initials: H
authors.lastname: Chen
authors.forename: Daniel
authors.initials: D
authors.lastname: Carrasco
authors.forename: Andrea
authors.initials: A
authors.lastname: Richardson
authors.forename: Shelia
authors.initials: S
authors.lastname: Violette
authors.forename: Tatiana
authors.initials: T
authors.lastname: Nikolskaya
authors.forename: Yuri
authors.initials: Y
authors.lastname: Nikolsky
authors.forename: Erica L
authors.initials: EL
authors.lastname: Bauerlein
authors.forename: William C
authors.initials: WC
authors.lastname: Hahn
authors.forename: Rebecca S
authors.initials: RS
authors.lastname: Gelman
authors.forename: Craig
authors.initials: C
authors.lastname: Allred
authors.forename: Mina J
authors.initials: MJ
authors.lastname: Bissell
authors.forename: Stuart
authors.initials: S
authors.lastname: Schnitt
authors.forename: Kornelia
authors.initials: K
authors.lastname: Polyak
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics / Q000473:pathology*; D002285:Carcinoma, Intraductal, Noninfiltrating / Q000235:genetics / Q000473:pathology*; D002448:Cell Adhesion; D019175:DNA Methylation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006706:Homeostasis; D006801:Humans; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide; D016212:Transforming Growth Factor beta / Q000235:genetics; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
chemical_list: D016212:Transforming Growth Factor beta; D016159:Tumor Suppressor Protein p53