Intracellular applications of analytical SERS spectroscopy and multispectral imaging.
The aim of this tutorial review is to give an overview of the state of the art of intracellular applications of analytical SERS spectroscopy. We pay particular attention to nanoparticle-based SERS spectroscopy since this currently dominates the published literature on non-disturbing analysis of live cells. We describe recent advances in this domain due to the development of multispectral imaging and to the combined use of SERRS (surface-enhanced resonance Raman scattering) and fluorescence spectroscopy. Finally, a perspective view is given on the tip-based approaches like tip-enhanced Raman spectroscopy (TERS) which allow micrometric and nanometric resolution.
Chourpa
Igor
I
Université François-Rabelais, Faculté de Pharmacie, Focalisation magnétique d'agents anticancéreux, 31 avenue Monge, F-37200 Tours, France. chourpa@univ-tours.fr
Lei
Franck H
FH
Dubois
Pierre
P
Manfait
Michel
M
Sockalingum
Ganesh D
GD
D000818:Animals; D002451:Cell Compartmentation / Q000502:physiology*; D006801:Humans; D007424:Intracellular Fluid / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008839:Microelectrodes; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman / Q000379:methods*