Inspiratory-phase short time scale synchrony in the brainstem slice is generated downstream of the pre-Bötzinger complex.
Respiratory neurons are synchronized on a long time scale to generate inspiratory and expiratory-phase activities that are critical for respiration. Long time scale synchrony within the respiratory network occurs on a time scale of more than hundreds of milliseconds to seconds. During inspiration, neurons are synchronized on a short time scale to produce synchronous oscillations, which shape the pattern of inspiratory motor output. This latter form of synchrony within the respiratory network spans a shorter time range of tens of milliseconds. In the neonatal mouse rhythmically active medullary slice preparation, we recorded bilateral inspiratory activity from hypoglossal (XII) rootlets to study where in the slice synchronous oscillations are generated. Based on previous work that proposed the origin of these oscillations, we tested the pre-Bötzinger complex (PreBötC) and the XII motor nucleus. Unilateral excitation of the PreBötC, via local application of a perfusate containing high K(+), increased mean inspiratory burst frequency bilaterally (296+/-66%; n=10, P<0.01), but had no effect on the relative power of oscillations. In contrast, unilateral excitation of the XII nucleus increased both mean peak integrated activity bilaterally (ipsilateral: 41+/-10%, P<0.01; contralateral: 17+/-7%; P<0.05, n=10) and oscillation power in the ipsilateral (50+/-17%, n=7, P<0.05), but not in the contralateral rootlet. Cross-correlation analysis of control inspiratory activity recorded from the left and right XII rootlets produced cross-correlation histograms with significant peaks centered around a time lag of zero and showed no subsidiary harmonic peaks. Coherence analysis of left and right XII rootlet recordings demonstrated that oscillations are only weakly coherent. Together, the findings from local application experiments and cross-correlation and coherence analyses indicate that short time scale synchronous oscillations recorded in the slice are likely generated in or immediately upstream of the XII motor nucleus.
Sebe
J Y
JY
Graduate Program in Neurobiology and Behavior, Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA 98195, USA. joysebe@gmail.com
Berger
A J
AJ
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology; D000818:Animals; D000831:Animals, Newborn; D004305:Dose-Response Relationship, Drug; D007839:Functional Laterality; D066298:In Vitro Techniques; D008526:Medulla Oblongata / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology*; D009434:Neural Pathways / Q000187:drug effects / Q000502:physiology; D010507:Periodicity; D011189:Potassium Chloride / Q000494:pharmacology; D012119:Respiration; D012125:Respiratory Center / Q000166:cytology*; D013057:Spectrum Analysis; D013997:Time Factors
D011189:Potassium Chloride