Next-generation high-density self-assembling functional protein arrays.
We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.
Ramachandran
Niroshan
N
Harvard Institute of Proteomics, Harvard Medical School, 320 Charles Street, Cambridge, Massachusetts 02141, USA.
Raphael
Jacob V
JV
Hainsworth
Eugenie
E
Demirkan
Gokhan
G
Fuentes
Manuel G
MG
Rolfs
Andreas
A
Hu
Yanhui
Y
LaBaer
Joshua
J
D000418:Albumins / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D019151:Peptide Library; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D040901:Proteomics / Q000379:methods* / Q000639:trends
D000418:Albumins; D018076:DNA, Complementary; D005456:Fluorescent Dyes; D019151:Peptide Library; D005982:Glutathione Transferase