Proteomic analysis of proteins associated with body mass and length in yellow perch, Perca flavescens.
The goal of commercial yellow perch aquaculture is to increase muscle mass which leads to increased profitability. The accumulation and degradation of muscle-specific gene products underlies the variability in body mass (BM) and length observed in pond-cultured yellow perch. Our objective was to apply a combination of statistical and proteomic technologies to identify intact and/or proteolytic fragments of muscle specific gene products involved in muscle growth in yellow perch. Seventy yellow perch randomly selected at 10, 12, 16, 20, and 26 wk of age were euthanized; BM and length were measured and a muscle sample taken. Muscle proteins were resolved using 5-20% gradient SDS-PAGE, stained with SYPRO Ruby and analyzed using TotalLab software. Data were analyzed using stepwise multiple regression with the dependent variables, BM and length and proportional OD of each band in a sample as a potential regressor. Eight bands associated with BM (R(2) = 0.84) and nine bands with length (R(2) = 0.85) were detected. Protein sequencing by nano-LC/MS/MS identified 20 proteins/peptides associated with BM and length. These results contribute the identification of gene products and/or proteolytic fragments associated with muscle growth in yellow perch.
Reddish
John Mark
JM
Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA.
St-Pierre
Normand
N
Nichols
Andy
A
Green-Church
Kari
K
Wick
Macdonald
M
D000818:Animals; D001823:Body Composition; D001835:Body Weight; D002853:Chromatography, Liquid / Q000379:methods; D004591:Electrophoresis, Polyacrylamide Gel; D029941:Fish Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D013058:Mass Spectrometry / Q000379:methods; D009132:Muscles / Q000378:metabolism; D009942:Organometallic Compounds / Q000494:pharmacology; D010471:Perches; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012044:Regression Analysis; D013997:Time Factors
D029941:Fish Proteins; D009942:Organometallic Compounds; D020543:Proteome; C515601:Sypro Ruby