title: Emergence of carbapenem resistance in Pseudomonas aeruginosa isolates from a patient with cystic fibrosis in the absence of carbapenem therapy.
abstract: The emergence of carbapenem-resistant Pseudomonas aeruginosa in the lung of a patient with cystic fibrosis was evaluated. A single strain of P. aeruginosa persisted during a 3-year study despite antipseudomonal treatment. A stepwise decrease in carbapenem susceptibility leading to resistance was observed in the absence of carbapenem treatment. These data suggest that chronic exposure to unrelated drug classes may be an important determinant for the emergence of carbapenem resistance in P. aeruginosa.
authors.affiliation: Center for Research in Anti-Infectives and Biotechnology, Creighton University School of Medicine, Omaha, Nebraska 68178, USA.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Wolter
authors.forename: Dee
authors.initials: D
authors.lastname: Acquazzino
authors.forename: Richard V
authors.initials: RV
authors.lastname: Goering
authors.forename: Paul
authors.initials: P
authors.lastname: Sammut
authors.forename: Noha
authors.initials: N
authors.lastname: Khalaf
authors.forename: Nancy D
authors.initials: ND
authors.lastname: Hanson
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015780:Carbapenems / Q000494:pharmacology*; D003550:Cystic Fibrosis / Q000150:complications*; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D018272:Porins / Q000235:genetics; D011552:Pseudomonas Infections / Q000382:microbiology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000302:isolation & purification; D013183:Sputum / Q000382:microbiology; D018440:beta-Lactam Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D015780:Carbapenems; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D018272:Porins; C077157:OprD protein, Pseudomonas aeruginosa