Identification of differentially expressed genes in the uterine endometrium on day 12 of the estrous cycle and pregnancy in pigs.
Maternal recognition of pregnancy in pigs occurs approximately on Day (D) 12 of pregnancy and is critical for embryo implantation. The presence of the conceptus in the uterine lumen during this period changes uterine endometrial function to prepare for attachment of the conceptus to the endometrial epithelial cells and maintain luteal function in the ovary. Although much is known about endometrial gene expression, the genes expressed in the uterine endometria and the cellular and molecular mechanisms of those gene products during the period of implantation and maternal recognition of pregnancy in pigs are still not completely defined. To better understand the interactions between the maternal uterus and conceptus during the implantation process, we searched genes differentially expressed in the endometria on D12 of pregnancy compared to those on D12 of the estrous cycle. A new reverse transcription-polymerase chain reaction (RT-PCR)-based method that involves annealing control primers (ACPs) was employed. Using 120 ACPs, we sequenced 12 differentially expressed genes (DEGs) and identified those genes using the Basic Local Alignment Search Tool (BLAST). Northern blot hybridization analysis confirmed the differential expression of those DEGs in the uterine endometrium. In situ hybridization analysis determined the cell-type specific expression of the DEGs in the uterine endometrium. Further analysis of the DEGs found in this study will provide insights into the cellular and molecular basis of maternal and fetal interactions during the period of maternal recognition of pregnancy in the pig. Mol. Reprod. Dev. 76: 75-84, 2009. (c) 2008 Wiley-Liss, Inc.
Ka
Hakhyun
H
Department of Biological Resources and Technology, Yonsei University, Wonju 220-710, Republic of Korea. hka@yonsei.ac.kr
Seo
Heewon
H
Kim
Mingoo
M
Choi
Yohan
Y
Lee
Chang-Kyu
CK
D000818:Animals; D015152:Blotting, Northern; D004717:Endometrium / Q000378:metabolism*; D030762:Estrous Cycle / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D017403:In Situ Hybridization; D011247:Pregnancy; D013552:Swine / Q000235:genetics*; D013997:Time Factors