A MAPK pathway is involved in the control of cortical granule reaction and mitosis during bovine fertilization.
In order to understand the mechanism by which mitogen-activated protein kinase (MAPK) regulates fertilization, we examined the effect of the MAPK pathway inhibitor U0126 on polyspermy, cortical granule reaction and mitosis in bovine oocytes during and after fertilization. Oocytes were treated with 30 microM U0126 for 30 min prior to insemination, or from 15 to 27 hr following insemination. Western blotting with antibodies that detect active, phosphorylated MAPK revealed that MAPK activity was decreased in U0126 treated oocytes. Oocytes that were treated with U0126 before insemination displayed a significantly higher incidence of polyspermic penetration and incomplete cortical granule reaction than that observed in untreated oocytes (P < 0.05). Exposure of oocytes to 30microM U0126 15-27 hr after insemination induced aberrant microtubule assembly and cell division, often resulting in the formation of two or three daughter cells with altered shapes and sizes. These results suggest that an ERK-like cascade is part of a mechanism that controls cortical granule reaction and the formation of the mitotic spindle following sperm penetration in the bovine.
Tae
Jin Cheol
JC
Mirae Biotech Co., Seoul, Korea.
Kim
Eun Young
EY
Jeon
Kilsoo
K
Lee
Keum Sil
KS
Lee
Chang Hyun
CH
Kim
Yeon Ok
YO
Park
Se Pill
SP
Kim
Nam-Hyung
NH
D000818:Animals; D015153:Blotting, Western; D002070:Butadienes / Q000494:pharmacology; D002417:Cattle; D005306:Fertilization / Q000187:drug effects / Q000502:physiology*; D016014:Linear Models; D018613:Microscopy, Confocal; D008870:Microtubules / Q000648:ultrastructure; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008938:Mitosis / Q000187:drug effects / Q000502:physiology*; D009570:Nitriles / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000648:ultrastructure*; D015398:Signal Transduction / Q000502:physiology*
D002070:Butadienes; D009570:Nitriles; C113580:U 0126; D020928:Mitogen-Activated Protein Kinases