title: Transient expression of phospholipase D1 during heart development in rats.
abstract: The expression of phospholipase D (PLD) isozymes was examined in the hearts of rats at different stages of development. Immunoprecipitation and Western blot analysis revealed weak PLD1 expression in the hearts of day 17 embryos. The level of PLD1 protein increased transiently 0 and 3 days postpartum, and declined gradually beginning 7 days after birth. Immunohistochemistry revealed weak PLD1 immunostaining in some cells at embryonic day 17. In contrast, some vascular endothelial cells and cardiomyocytes were immunostained typically at days 0, 3, and 7 after birth. After postnatal day 21, weak PLD1 expression was immunodetected in some vascular endothelial cells and cardiomyocytes. This suggests that the PLD1 protein in the heart is strongly associated with the early postnatal development of the heart in rats.
authors.affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Heechul
authors.initials: H
authors.lastname: Kim
authors.forename: Seungjoon
authors.initials: S
authors.lastname: Kim
authors.forename: Yongduk
authors.initials: Y
authors.lastname: Lee
authors.forename: Min Kyoung
authors.initials: MK
authors.lastname: Shin
authors.forename: Do Sik
authors.initials: do S
authors.lastname: Min
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D005786:Gene Expression Regulation; D006321:Heart / Q000196:embryology*; D009206:Myocardium; D010739:Phospholipase D / Q000378:metabolism*; D051381:Rats
chemical_list: D010739:Phospholipase D; C106801:phospholipase D1