title: Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties.
abstract: Viscosin (1), an effective surface-active cyclic lipopeptide, was efficiently recovered from Pseudomonas libanensis M9-3 with a simple purification protocol. A major pigment also obtained during this process was identified as phenazine-1-carboxylic acid. The critical micelle concentration (cmc) of viscosin was determined to be 54 mg L (-1), and the minimum surface tension between air and water at the cmc was 28 mN m (-1). Viscosin forms stable emulsions even at low concentrations (7.5 mg L (-1)), and the conditional stability constant for a cadmium-viscosin complex was determined to be 5.87. The physicochemical properties measured for viscosin are similar to other well-studied biosurfactants such as rhamnolipid and surfactin. Viscosin inhibited migration of the metastatic prostate cancer cell line, PC-3M, without visible toxicity. These properties suggest the potential of viscosin in environmental and biomedical applications.
authors.affiliation: Department of Microbiology, Guru Nanak dev university, Amritsar-143005, Punjab, India.
authors.forename: Harvinder S
authors.initials: HS
authors.lastname: Saini
authors.forename: Blanca E
authors.initials: BE
authors.lastname: Barragán-Huerta
authors.forename: Ariel
authors.initials: A
authors.lastname: Lebrón-Paler
authors.forename: Jeanne E
authors.initials: JE
authors.lastname: Pemberton
authors.forename: Refugio R
authors.initials: RR
authors.lastname: Vázquez
authors.forename: Anna M
authors.initials: AM
authors.lastname: Burns
authors.forename: Marilyn T
authors.initials: MT
authors.lastname: Marron
authors.forename: Chistopher J
authors.initials: CJ
authors.lastname: Seliga
authors.forename: A A Leslie
authors.initials: AA
authors.lastname: Gunatilaka
authors.forename: Raina M
authors.initials: RM
authors.lastname: Maier
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008297:Male; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011549:Pseudomonas / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000970:Antineoplastic Agents; D010456:Peptides, Cyclic; D013501:Surface-Active Agents; C062796:viscosin