Platelet protein interactions: map, signaling components, and phosphorylation groundstate.
OBJECTIVE
Assembly of a comprehensive proteome and transcriptome database of human platelets, derivation of a model of the platelet-specific interactome, and generation of a functional interaction map of platelet phosphorylations and kinases.


RESULTS
Interactions are derived from literature-curated data from HPRD and yeast two hybrid (Y2H) and mapped to platelet-specific expression data (SAGE or proteome). From this a cell-type specific model of platelet proteins and protein-protein interactions is derived. The obtained inventory of platelet-specific proteins includes key domains, protein GO annotations, and receptors. Collected interactions point to new platelet signaling components, actin remodeling processes, and pharmacological targets and offer incentives for further studies (eg, on the IPP complex). Integration of platelet-specific phosphoproteins and the characterization of the platelet kinase repertoire sketch a first outline of kinase signaling in human platelets.


CONCLUSIONS
A first view of the platelet interactome, platelet phosphorylation, and platelet kinome is available from the in silico data.
Dittrich
Marcus
M
Department of Bioinformatics, Biocenter, University of Würzburg, Am Hubland, Würzburg D-97074, Germany.
Birschmann
Ingvild
I
Mietner
Silke
S
Sickmann
Albert
A
Walter
Ulrich
U
Dandekar
Thomas
T
D001792:Blood Platelets / Q000201:enzymology / Q000378:metabolism*; D019295:Computational Biology; D003196:Computer Graphics; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006801:Humans; D010766:Phosphorylation; D010980:Platelet Membrane Glycoproteins / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011494:Protein Kinases / Q000378:metabolism; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D040901:Proteomics; D015398:Signal Transduction / Q000235:genetics; D014584:User-Computer Interface
D010980:Platelet Membrane Glycoproteins; D011506:Proteins; D011494:Protein Kinases