Viral accessory protein X stimulates the assembly of functional Borna disease virus polymerase complexes.
The Borna disease virus (BDV) proteins X and P are translated from a bicistronic viral mRNA. Here, it was shown that the rescue of recombinant BDV from cDNA was enhanced approximately eightfold if reconstitution of the viral polymerase complex was performed with an expression vector encoding X and P rather than P alone. The results provide evidence that appropriate amounts of X reduce the previously reported high sensitivity of the BDV polymerase to imbalances between the viral proteins N and P. These data indicate that X buffers an unfavourable excess of P, thereby stimulating the assembly of functional BDV polymerase complexes.
Poenisch
Marion
M
Department of Virology, University of Freiburg, D-79104 Freiburg, Germany.
Staeheli
Peter
P
Department of Virology, University of Freiburg, D-79104 Freiburg, Germany.
Schneider
Urs
U
Department of Virology, University of Freiburg, D-79104 Freiburg, Germany.
D000818:Animals; D001890:Borna Disease / Q000821:virology*; D001891:Borna disease virus / Q000737:chemistry / Q000502:physiology*; D002460:Cell Line; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D006801:Humans; D009698:Nucleoproteins; D054334:Viral Regulatory and Accessory Proteins / Q000502:physiology*; D014779:Virus Replication
D009698:Nucleoproteins; D054334:Viral Regulatory and Accessory Proteins; D012321:DNA-Directed RNA Polymerases