title: Glycogen branching enzyme: a novel deltamethrin resistance-associated gene from Culex pipiens pallens.
abstract: The glycogen branching enzyme (GBE) gene has been cloned from Culex pipiens pallens. An open reading frame of 2,070 bp was found to encode a putative 689 amino acid protein. The deduced amino acid sequence shares 82% and 60% identity with GBE genes from Aedes aegypti and Homo sapiens. Transcript expression of GBE was determined by real-time polymerase chain reaction in deltamethrin-susceptible and deltamethrin-resistant strains of the Culex mosquito. The results demonstrated that this gene has 19.7-fold higher expression in deltamethrin-resistant strain. Unexpectedly, the following study showed that cell proliferation decreased obviously in the GBE overexpression group compared to the empty vector control or the unrelated gene when treated by deltamethrin in the mosquito C6/36 cells whose mechanism is still unexplained. Our study provides the first direct evidence that GBE gene may play some role in the development of deltamethrin resistance in C. pipiens pallens.
authors.affiliation: Department of Pathogen Biology, Nanjing Medical University, Nanjing, Jiangsu Province, 210029, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.forename: Mifang
authors.initials: M
authors.lastname: Yang
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.forename: Jin
authors.initials: J
authors.lastname: Qian
authors.forename: Donghui
authors.initials: D
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Ma
authors.forename: Changliang
authors.initials: C
authors.lastname: Zhu
mesh_terms: D015061:1,4-alpha-Glucan Branching Enzyme / Q000235:genetics* / Q000378:metabolism; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D003001:Cloning, Molecular; D003465:Culex / Q000187:drug effects* / Q000201:enzymology* / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics*; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007306:Insecticides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D017422:Sequence Analysis, DNA; D014162:Transfection
chemical_list: D019476:Insect Proteins; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017180:decamethrin; D015061:1,4-alpha-Glucan Branching Enzyme