title: Purified extract from Clematis mandshurica prevents adenoviral-TRAIL induced apoptosis on rat articular chondrocytes.
abstract: Since TNF-related apoptosis inducing ligand (TRAIL) is one of several apoptotic stimuli on articular chondrocytes, the modulation of the mechanism mediated by TRAIL could be considered as a novel strategy for the treatment of osteoarthritis (OA). Previous studies demonstrated that Clematis mandshurica prevents staurosporin-induced apoptosis in articular chondrocytes. This study was undertaken to examine whether Clematis mandshurica could prevent TRAIL-induced apoptosis in articular chondrocytes. Our data show that Clematis mandshurica prevents adenoviral TRAIL (Ad-TRAIL)-induced apoptosis in primary cultured articular chondrocytes. Clematis mandshurica prevents Ad-TRAIL-induced down-regulation of 14-3-3 and phosphorylated Akt. In addition, Clematis mandshurica treatment prevents the Ad-TRAIL-induced reduction of the interactions between 14-3-3 with phospho-ser112-Bad and phospho-ser136-Bad, and BcL-xL with phospho-ser155-Bad. A better understanding of the mechanism underlying inhibition of apoptosis in OA chondrocytes by Clematis mandshurica might lead to the development of a new therapeutic strategy for OA.
authors.affiliation: Department of Rheumatology, Dong-A University College of Medicine and Institute of Medical Science, Busan, South Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Lee
authors.forename: Hye Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Jeong Bon
authors.initials: JB
authors.lastname: Moon
authors.forename: Sun Mee
authors.initials: SM
authors.lastname: Choi
authors.forename: Duk Kyu
authors.initials: DK
authors.lastname: Kim
authors.forename: In Ryoung
authors.initials: IR
authors.lastname: Kim
authors.forename: Won Chul
authors.initials: WC
authors.lastname: Choi
authors.forename: Bong Soo
authors.initials: BS
authors.lastname: Park
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D000256:Adenoviridae; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D039324:Clematis; D003864:Depression, Chemical; D015536:Down-Regulation; D007596:Joints / Q000166:cytology*; D051058:Oncogene Protein v-akt / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy / Q000209:etiology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000037:antagonists & inhibitors* / Q000494:pharmacology
chemical_list: D048948:14-3-3 Proteins; D010936:Plant Extracts; D053221:TNF-Related Apoptosis-Inducing Ligand; D051058:Oncogene Protein v-akt