title: Drosophila arginine methyltransferase 1 (DART1) is an ecdysone receptor co-repressor.
abstract: Histone arginine methylation is an epigenetic marker that regulates gene expression by defining the chromatin state. Arginine methyltransferases, therefore, serve as transcriptional co-regulators. However, unlike other transcriptional co-regulators, the physiological roles of arginine methyltransferases are poorly understood. Drosophila arginine methyltransferase 1 (DART1), the mammalian PRMT1 homologue, methylates the arginine residue of histone H4 (H4R3me2). Disruption of DART1 in Drosophila by imprecise P-element excision resulted in low viability during metamorphosis in the pupal stages. In the pupal stage, an ecdysone hormone signal is critical for developmental progression. DART1 interacted with the nuclear ecdysone receptor (EcR) in a ligand-dependent manner, and co-repressed EcR in intact flies. These findings suggest that DART1, a histone arginine methyltransferase, is a co-repressor of EcR that is indispensable for normal pupal development in the intact fly.
authors.affiliation: The Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Kimura
authors.forename: Shun
authors.initials: S
authors.lastname: Sawatsubashi
authors.forename: Saya
authors.initials: S
authors.lastname: Ito
authors.forename: Alexander
authors.initials: A
authors.lastname: Kouzmenko
authors.forename: Eriko
authors.initials: E
authors.lastname: Suzuki
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhao
authors.forename: Kaoru
authors.initials: K
authors.lastname: Yamagata
authors.forename: Masahiko
authors.initials: M
authors.lastname: Tanabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Ueda
authors.forename: Sari
authors.initials: S
authors.lastname: Fujiyama
authors.forename: Takuya
authors.initials: T
authors.lastname: Murata
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Matsukawa
authors.forename: Ken-Ichi
authors.initials: K
authors.lastname: Takeyama
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yaegashi
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Kato
mesh_terms: D000818:Animals; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D005804:Genes, Lethal; D006657:Histones / Q000378:metabolism; D047468:Immunoprecipitation; D008745:Methylation; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism*; D009154:Mutation; D011987:Receptors, Steroid / Q000378:metabolism*; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D006657:Histones; D011987:Receptors, Steroid; D012097:Repressor Proteins; C055469:ecdysone receptor; C487898:Art1 protein, Drosophila; D008780:Methyltransferases