title: Prognostic value of a proliferation index including MIB1 and argyrophilic nucleolar organizer regions proteins in node-negative breast cancer.
abstract: OBJECTIVE
  This study was designed to evaluate a cell proliferation marker, including the percentage of cycling cells (MIB1), and the duration of the cell cycle (assessed by argyrophilic nucleolar organizer regions proteins [AgNORs] measurement).
  
  
  METHODS
  We included 90 patients with invasive node-negative breast cancer. None received chemotherapy. With the help of a double-staining technique, a proliferation index (PI) was determined by multiplying the percentage of MIB1-positive cells by the mean area of the AgNORs present in those MIB1-positive cells. PI was evaluated for its impact on overall survival (OS) and disease-free survival (DFS).
  
  
  RESULTS
  We demonstrated that PI was correlated to OS. For DFS, it conserved its high prognostic value only in univariate analysis. The global amount of AgNORs was more discriminative for DFS.
  
  
  CONCLUSIONS
  PI and AgNOR quantification supplied additional prognosis information in node-negative patients, and we propose to integrate them in further studies.
authors.affiliation: Department of Gynecology and Obstetrics, Hospital of Soissons, Soissons, France. pascal.abboud@ch-soissons.fr
authors.forename: Pascal
authors.initials: P
authors.lastname: Abboud
authors.forename: Marianne
authors.initials: M
authors.lastname: Lorenzato
authors.forename: Damien
authors.initials: D
authors.lastname: Joly
authors.forename: Christian
authors.initials: C
authors.lastname: Quereux
authors.forename: Philippe
authors.initials: P
authors.lastname: Birembaut
authors.forename: Dominique
authors.initials: D
authors.lastname: Ploton
mesh_terms: D000368:Aged; D034961:Antigens, Nuclear / Q000032:analysis*; D001943:Breast Neoplasms / Q000401:mortality* / Q000503:physiopathology / Q000601:surgery; D002467:Cell Nucleus / Q000737:chemistry*; D049109:Cell Proliferation; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D007150:Immunohistochemistry; D019394:Ki-67 Antigen; D015999:Multivariate Analysis; D009363:Neoplasm Proteins; D011379:Prognosis; D044767:Ubiquitin-Protein Ligases / Q000032:analysis*
chemical_list: D034961:Antigens, Nuclear; D019394:Ki-67 Antigen; D009363:Neoplasm Proteins; C051963:nucleolar organizer region associated proteins; C513496:MIB1 ligase, human; D044767:Ubiquitin-Protein Ligases