Preliminary application and evaluation of loop-mediated isothermal amplification (LAMP) for detection of bovine theileriosis and trypanosomosis in Tanzania.
The sensitivity of LAMP, PCR and microscopy to detect Theileria spp. and Trypanosoma congolense in field-derived bovine blood samples from Tanzania was evaluated and compared. No parasites were detected by microscopy. Furthermore, no bovine Theileria spp. were detected by LAMP and PCR from all the 24 samples collected from Arusha. Four and one out of 24 samples were positive for Theileria congolense infection by LAMP and PCR respectively while, 18 and nine out of 40 samples from Dar es Salaam were positive by LAMP and PCR for Theileria spp. Infection, respectively. Although all samples from Dar es Salaam were negative for Trypanosoma congolense infections by PCR, 12 out of 40 samples were LAMP positive. Whilst PCR is an established gene amplification method for the detection of Theileria and trypanosome parasites, this study introduces LAMP as an alternative molecular diagnostic tool that could be used in large-scale epidemiological surveys.
Thekisoe
O M M
OM
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.
Omolo
J D
JD
Swai
E S
ES
Hayashida
K
K
Zhang
J
J
Sugimoto
C
C
Inoue
N
N
D000818:Animals; D002417:Cattle; D002418:Cattle Diseases / Q000175:diagnosis* / Q000453:epidemiology; D016054:DNA, Protozoan / Q000737:chemistry*; D008853:Microscopy / Q000379:methods / Q000662:veterinary; D021141:Nucleic Acid Amplification Techniques / Q000379:methods / Q000662:veterinary*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013636:Tanzania / Q000453:epidemiology; D016794:Theileria / Q000302:isolation & purification; D013801:Theileriasis / Q000175:diagnosis* / Q000453:epidemiology; D014345:Trypanosoma / Q000302:isolation & purification; D014354:Trypanosomiasis, Bovine / Q000175:diagnosis* / Q000453:epidemiology
D016054:DNA, Protozoan