title: Caffeic acid phenethyl ester preferentially enhanced radiosensitizing and increased oxidative stress in medulloblastoma cell line.
abstract: OBJECTIVE
  Caffeic acid phenethyl ester (CAPE), an active component of propolis, was recently reported to have radiosensitizing effects on medulloblastoma (MB) cells. However, the mechanisms of radiosensitivity involved in medulloblastoma cells are still unclear. The specific aim of this study was to investigate the role of CAPE-induced oxidative stress to influence of radiosensitivity and anti-proliferative effects in medulloblastoma cells.
  
  
  METHODS
  Medulloblastoma (Daoy) cells were treated with CAPE in different concentrations and assessed for cell viability. The following were also evaluated: migratory ability, reduced glutathione (GSH) level, reactive oxygen species (ROS) level, nuclear factor-kappaB (NF-kappaB) activity, and apoptosis in CAPE alone, radiation alone, or radiation combined with CAPE in Daoy cells.
  
  
  RESULTS
  The results indicated that CAPE inhibited the growth of Daoy cells. CAPE treatment in Daoy cells could effectively decrease glutathione reductase and significantly increase glutathione peroxidase. Radiation-activated NF-kappaB was reversed by CAPE pretreatment. Finally, the result of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay showed that CAPE treatment can enhance radiation-induced apoptosis in Daoy cells.
  
  
  CONCLUSIONS
  Our study demonstrated the anti-proliferative and radiosensitizing effects of CAPE on MB cells, which may be achievable through depleting GSH, increased ROS activity, and inhibiting NF-kappaB activity.
authors.affiliation: Institute of Clinical Medicine, National Yang-Ming University, and Department of Physical Medicine and Rehabilitation, The Neurological Institute, Taipei Veterans General Hospital, Taipei, Taiwan.
authors.forename: Yi-Yen
authors.initials: YY
authors.lastname: Lee
authors.forename: Chung-Lan
authors.initials: CL
authors.lastname: Kao
authors.forename: Ping-Hsing
authors.initials: PH
authors.lastname: Tsai
authors.forename: Tung-Hu
authors.initials: TH
authors.lastname: Tsai
authors.forename: Shih-Hwa
authors.initials: SH
authors.lastname: Chiou
authors.forename: Wei-Fong
authors.initials: WF
authors.lastname: Wu
authors.forename: Hung-Hai
authors.initials: HH
authors.lastname: Ku
authors.forename: Tai-Tong
authors.initials: TT
authors.lastname: Wong
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D002109:Caffeic Acids / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005978:Glutathione / Q000187:drug effects; D006801:Humans; D020287:In Situ Nick-End Labeling; D008527:Medulloblastoma / Q000378:metabolism*; D016328:NF-kappa B / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D010626:Phenylethyl Alcohol / Q000031:analogs & derivatives* / Q000494:pharmacology; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D002109:Caffeic Acids; D016328:NF-kappa B; D011838:Radiation-Sensitizing Agents; D017382:Reactive Oxygen Species; C055494:caffeic acid phenethyl ester; D005978:Glutathione; D010626:Phenylethyl Alcohol