Two immunoassay formats for fully automated CRP detection in human serum.
Immunoassays are a proven approach towards fast, sensitive, cost-effective and easy-to-use analytical systems which are able to measure a variety of interesting analytes, especially in medical diagnostics. Herein, we report two assay formats, binding inhibition and sandwich assay format, for detection of C-reactive protein (CRP) in human serum. Both assays were characterised and compared with respect to their suitability and adaption into a complete sensor system. An automated, optical biosensor system, based on evanescent field technology, was used to carry out a full threefold calibration in each case. Owing to the resulting working ranges, 0.044-2.9 mg L(-1) and 0.13-22.9 mg L(-1), respectively, the assays qualify for use in detecting high-sensitivity CRP (C-reactive protein).
Albrecht
Christiane
C
Institute of Physical and Theoretical Chemistry (IPTC), Eberhard-Karls-University of Tuebingen, Auf der Morgenstelle 8, 72076, Tuebingen, Germany. christiane.albrecht@ipc.uni-tuebingen.de
Kaeppel
Nina
N
Gauglitz
Guenter
G
D001322:Autoanalysis; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002097:C-Reactive Protein / Q000032:analysis*; D019411:Clinical Laboratory Techniques / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D055095:Optics and Photonics; D012016:Reference Values; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry*; D013997:Time Factors
D005456:Fluorescent Dyes; D002097:C-Reactive Protein