The activity and stability of cell-associated activity of bovicin HC5, a bacteriocin from Streptococcus bovis HC5.
Streptococcus bovis HC5 cultures released a broad spectrum lantibiotic, bovicin HC5, into the cell-fee culture supernatant after they reached stationary phase, but most of the antibacterial activity remained cell-associated. Cell-associated bovicin HC5 was more resistant to degradation by Pronase E than the cell-free activity. Acidic NaCl (pH 2.0, 100 mM) did not release all of the cell-associated activity, and cells that were sequentially treated with acidic NaCl and Pronase E still had antibacterial activity. Cell-associated activity retained after acidic NaCl treatment was still able to catalyze potassium efflux from S. bovis JB1, a sensitive strain. These results indicate that cell-associated bovicin HC5 is more active and stable than cell-free bovicin HC5.
Xavier
Bruno M
BM
Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
Houlihan
Adam J
AJ
Russell
James B
JB
D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D006863:Hydrogen-Ion Concentration; D009997:Osmotic Pressure; D011188:Potassium / Q000378:metabolism; D011402:Pronase / Q000378:metabolism; D016985:Streptococcus bovis / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; C514465:bovicin HC5, Streptococcus bovis HC5; D011402:Pronase; D011188:Potassium