title: Fabrication and application of single nucleotide polymorphisms library on magnetic nanoparticles using adaptor PCR.
abstract: We have developed a novel approach to fabricate single nucleotide polymorphisms (SNPs) library on magnetic nanoparticles (MNPs) based on adaptor PCR. Each SNP locus in the library was interrogated by hybridization with a pair of allele specific dual-color fluorescence (Cy3, Cy5) probes to determine SNP. Two SNPs loci (M235T and A-6G) associated with essential hypertension in the angiotensinogen (AGT) gene were detected by this method and their fluorescent signals were quantified. The fluorescent ratios (match probe: mismatch probe signal) of homozygous genotypes were over 3.0, whereas heterozygous genotypes had ratios near to 1.0. Without any complex multiplex PCR procedure, it is a simple, efficient and reliable method for the multiplex SNPs detection using limited amount of DNA samples from individuals.
authors.affiliation: Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412008, China.
authors.forename: Hongna
authors.initials: H
authors.lastname: Liu
authors.forename: Song
authors.initials: S
authors.lastname: Li
authors.forename: Meiju
authors.initials: M
authors.lastname: Ji
authors.forename: Libo
authors.initials: L
authors.lastname: Nie
authors.forename: Jianrong
authors.initials: J
authors.lastname: Chen
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Miao
authors.forename: Nongyue
authors.initials: N
authors.lastname: He
mesh_terms: D001483:Base Sequence; D017931:DNA Primers; D008280:Magnetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D020641:Polymorphism, Single Nucleotide
chemical_list: D017931:DNA Primers