Efficient purification of the biosurfactant viscosin from Pseudomonas libanensis strain M9-3 and its physicochemical and biological properties.
Viscosin (1), an effective surface-active cyclic lipopeptide, was efficiently recovered from Pseudomonas libanensis M9-3 with a simple purification protocol. A major pigment also obtained during this process was identified as phenazine-1-carboxylic acid. The critical micelle concentration (cmc) of viscosin was determined to be 54 mg L (-1), and the minimum surface tension between air and water at the cmc was 28 mN m (-1). Viscosin forms stable emulsions even at low concentrations (7.5 mg L (-1)), and the conditional stability constant for a cadmium-viscosin complex was determined to be 5.87. The physicochemical properties measured for viscosin are similar to other well-studied biosurfactants such as rhamnolipid and surfactin. Viscosin inhibited migration of the metastatic prostate cancer cell line, PC-3M, without visible toxicity. These properties suggest the potential of viscosin in environmental and biomedical applications.
Saini
Harvinder S
HS
Department of Microbiology, Guru Nanak dev university, Amritsar-143005, Punjab, India.
Barragán-Huerta
Blanca E
BE
Lebrón-Paler
Ariel
A
Pemberton
Jeanne E
JE
Vázquez
Refugio R
RR
Burns
Anna M
AM
Marron
Marilyn T
MT
Seliga
Chistopher J
CJ
Gunatilaka
A A Leslie
AA
Maier
Raina M
RM
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D008297:Male; D015394:Molecular Structure; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D011549:Pseudomonas / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects
D000970:Antineoplastic Agents; D010456:Peptides, Cyclic; D013501:Surface-Active Agents; C062796:viscosin