Comparison of conditioning regimens for alveolar macrophage reconstitution and innate immune function post bone marrow transplant.
The authors compared efficiency of alveolar macrophage (AM) reconstitution from donor bone marrow post transplant following 4 chemotherapy conditioning regimens and 2 total body irradiation (TBI) regimens. TBI regimens are more effective in inducing AM reconstitution from donor marrow. However, mice conditioned with 13 Gy split-dose TBI or a dual-chemotherapy regimen (25 mg/kg busulfan x 4 days plus cyclophosphamide 100 mg/kg x 2 days) both demonstrate significant AM repopulation from donor marrow. Additionally, both protocols resulted in impaired pulmonary host defense associated with overproduction of prostaglandin E(2) and I(2) by AMs and impaired AM phagocytosis post bone marrow transplant.
Hubbard
Leah L N
LL
Immunology Graduate Program, University of Michigan, Ann Arbor, Michigan, USA.
Ballinger
Megan N
MN
Wilke
Carol A
CA
Moore
Bethany B
BB
D000818:Animals; D016026:Bone Marrow Transplantation / Q000502:physiology*; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002066:Busulfan / Q000009:adverse effects; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D011464:Epoprostenol / Q000378:metabolism; D008168:Lung / Q000187:drug effects / Q000382:microbiology / Q000473:pathology; D016676:Macrophages, Alveolar / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D019653:Myeloablative Agonists / Q000009:adverse effects; D010587:Phagocytosis / Q000187:drug effects; D011650:Pulmonary Alveoli / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D013047:Specific Pathogen-Free Organisms; D019172:Transplantation Conditioning; D014916:Whole-Body Irradiation
D019653:Myeloablative Agonists; D011464:Epoprostenol; D002066:Busulfan; D015232:Dinoprostone