title: Alpha1,4GlcNAc-capped mucin-type O-glycan inhibits cholesterol alpha-glucosyltransferase from Helicobacter pylori and suppresses H. pylori growth.
abstract: Helicobacter pylori infects over half of the world's population and is thought to be a leading cause of gastric ulcer, gastric carcinoma, and gastric malignant lymphoma of mucosa-associated lymphoid tissue type. Previously, we reported that a gland mucin (MUC6) present in the lower portion of the gastric mucosa containing alpha1,4-N-acetylglucosamine (alpha1,4GlcNAc)-capped core 2-branched O-glycans suppresses H. pylori growth by inhibiting the synthesis of alpha-glucosyl cholesterol, a major constituent of the H. pylori cell wall (Kawakubo et al. 2004. Science. 305:1003-1006). Therefore, we cloned the genomic DNA encoding cholesterol alpha-glucosyltransferase (HP0421) and expressed its soluble form in Escherichia coli. Using this soluble HP0421, we show herein that HP0421 sequentially acts on uridine diphosphoglucose and cholesterol in an ordered Bi-Bi manner. We found that competitive inhibition of HP0421 by alpha1,4GlcNAc-capped core 2-branched O-glycan is much more efficient than noncompetitive inhibition by newly synthesized alpha-glucosyl cholesterol. Utilizing synthetic oligosaccharides, alpha-glucosyl cholesterol, and monosaccharides, we found that alpha1,4GlcNAc-capped core 2-branched O-glycan most efficiently inhibits H. pylori growth. These findings together indicate that alpha1,4GlcNAc-capped O-glycans suppress H. pylori growth by inhibiting HP0421, and that alpha1,4GlcNAc-capped core 2 O-glycans may be useful to treat patients infected with H. pylori.
authors.affiliation: Tumor Microenvironment Program, Glycobiology Unit, Cancer Center, Burnham Institute for Medical Research, La Jolla, CA 92037, USA.
authors.forename: Heeseob
authors.initials: H
authors.lastname: Lee
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.forename: Hitomi
authors.initials: H
authors.lastname: Hoshino
authors.forename: Yuki
authors.initials: Y
authors.lastname: Ito
authors.forename: Motohiro
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Jun
authors.initials: J
authors.lastname: Nakayama
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeberger
authors.forename: Minoru
authors.initials: M
authors.lastname: Fukuda
mesh_terms: D000117:Acetylglucosamine / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D002784:Cholesterol / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005964:Glucosyltransferases / Q000037:antagonists & inhibitors*; D016480:Helicobacter pylori / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D009844:Oligosaccharides / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000037:antagonists & inhibitors; D012995:Solubility; D014532:Uridine Diphosphate Glucose / Q000378:metabolism
chemical_list: D009844:Oligosaccharides; D011134:Polysaccharides; D011994:Recombinant Proteins; D002784:Cholesterol; D005964:Glucosyltransferases; C514935:Hp0421 protein, Helicobacter pylori; D014532:Uridine Diphosphate Glucose; D000117:Acetylglucosamine