title: Mammalian complex I: a regulable and vulnerable pacemaker in mitochondrial respiratory function.
abstract: In this paper the regulatory features of complex I of mammalian and human mitochondria are reviewed. In a variety of mitotic cell-line cultures, activation in vivo of the cAMP cascade, or direct addition of cAMP, promotes the NADH-ubiquinone oxidoreductase activity of complex I and lower the cellular level of ROS. These effects of cAMP are found to be associated with PKA-mediated serine phosphorylation in the conserved C-terminus of the subunit of complex I encoded by the nuclear gene NDUFS4. PKA mediated phosphorylation of this Ser in the C-terminus of the protein promotes its mitochondrial import and maturation. Mass-spectrometry analysis of the phosphorylation pattern of complex I subunits is also reviewed.
authors.affiliation: Department of Medical Biochemistry, Biology and Physics (DIBIFIM), University of Bari, Italy. papabchm@cimedoc.uniba.it
authors.forename: Sergio
authors.initials: S
authors.lastname: Papa
authors.forename: Domenico
authors.initials: D
authors.lastname: De Rasmo
authors.forename: Salvatore
authors.initials: S
authors.lastname: Scacco
authors.forename: Anna
authors.initials: A
authors.lastname: Signorile
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Technikova-Dobrova
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Palmisano
authors.forename: Anna Maria
authors.initials: AM
authors.lastname: Sardanelli
authors.forename: Francesco
authors.initials: F
authors.lastname: Papa
authors.forename: Damiano
authors.initials: D
authors.lastname: Panelli
authors.forename: Raffaella
authors.initials: R
authors.lastname: Scaringi
authors.forename: Arcangela
authors.initials: A
authors.lastname: Santeramo
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D000242:Cyclic AMP / Q000378:metabolism; D042967:Electron Transport Complex I / Q000737:chemistry / Q000378:metabolism*; D006706:Homeostasis; D007700:Kinetics; D008322:Mammals; D008928:Mitochondria / Q000201:enzymology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010101:Oxygen Consumption; D017386:Sequence Homology, Amino Acid
chemical_list: D000242:Cyclic AMP; D042967:Electron Transport Complex I