title: Electron paramagnetic resonance oxygen image hypoxic fraction plus radiation dose strongly correlates with tumor cure in FSa fibrosarcomas.
abstract: OBJECTIVE
  Tumor hypoxia has long been known to produce resistance to radiation. In this study, electron paramagnetic resonance (EPR) oxygen imaging was investigated for its power to predict the success of tumor control according to tumor oxygenation level and radiation dose.
  
  
  METHODS
  A total of 34 EPR oxygen images were obtained from the legs of C3H mice bearing 0.5-cm(3) FSa fibrosarcomas under both normal (air breathing) and clamped tumor conditions. Under the same conditions as those during which the images were obtained, the tumors were irradiated to a variety of doses near the FSa dose at which 50% of tumors were cured. Tumor tissue was distinguished from normal tissue using co-registration of the EPR oxygen images with spin-echo magnetic resonance imaging of the tumor and/or stereotactic localization. The tumor voxel statistics in the EPR oxygen image included the mean and median partial pressure of oxygen and the fraction of tumor voxels below the specified partial pressure of oxygen values of 3, 6, and 10 mm Hg. Bivariate logistic regression analysis using the radiation dose and each of the EPR oxygen image statistics to determine which best separated treatment failure from success.
  
  
  RESULTS
  The measurements of the dose at which 50% of tumors were cured were similar to those found in published data for this syngeneic tumor. Bivariate analysis of 34 tumors demonstrated that tumor cure correlated with dose (p = 0.004) and with a <10 mm Hg hypoxic fraction (p = 0.023).
  
  
  CONCLUSIONS
  Our results have shown that, together, radiation dose and EPR image hypoxic fraction separate the population of FSa fibrosarcomas that are cured from those that fail, thus predicting curability.
authors.affiliation: Department of Radiation and Cellular Oncology, University of Chicago Pritzker School of Medicine, Chicago, IL 60637, USA.
authors.forename: Martyna
authors.initials: M
authors.lastname: Elas
authors.forename: Rebecca
authors.initials: R
authors.lastname: Bell
authors.forename: Danielle
authors.initials: D
authors.lastname: Hleihel
authors.forename: Eugene D
authors.initials: ED
authors.lastname: Barth
authors.forename: Colin
authors.initials: C
authors.lastname: McFaul
authors.forename: Chad R
authors.initials: CR
authors.lastname: Haney
authors.forename: Joanna
authors.initials: J
authors.lastname: Bielanska
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pustelny
authors.forename: Kang-Hyun
authors.initials: KH
authors.lastname: Ahn
authors.forename: Charles A
authors.initials: CA
authors.lastname: Pelizzari
authors.forename: Masha
authors.initials: M
authors.lastname: Kocherginsky
authors.forename: Howard J
authors.initials: HJ
authors.lastname: Halpern
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D005354:Fibrosarcoma / Q000378:metabolism* / Q000503:physiopathology / Q000532:radiotherapy*; D051379:Mice; D008809:Mice, Inbred C3H; D010100:Oxygen / Q000032:analysis*; D010101:Oxygen Consumption / Q000502:physiology; D010313:Partial Pressure; D011836:Radiation Tolerance / Q000502:physiology*; D011878:Radiotherapy / Q000379:methods; D011879:Radiotherapy Dosage; D012074:Remission Induction
chemical_list: D010100:Oxygen