title: Immune responses to Pneumocystis murina are robust in healthy mice but largely absent in CD40 ligand-deficient mice.
abstract: Pneumocystis is a pathogen of immunocompromised hosts but can also infect healthy hosts, in whom infection is rapidly controlled and cleared. Microarray methods were used to examine differential gene expression in the lungs of C57BL/6 and CD40 ligand knockout (CD40L-KO) mice over time following exposure to Pneumocystis murina. Immunocompetent C57BL/6 mice, which control and clear infection efficiently, showed a robust response to infection characterized by the up-regulation of 349 primarily immune response-associated genes. Temporal changes in the expression of these genes identified an early (Week 2), primarily innate response, which waned before the infection was controlled; this was followed by primarily adaptive immune responses that peaked at Week 5, which coincided with clearance of the infection. In conjunction with the latter, there was an increased expression of B cell-associated (Ig) genes at Week 6 that persisted through 11 weeks. In contrast, CD40L-KO mice, which are highly susceptible to developing severe Pneumocystis pneumonia, showed essentially no up-regulation of immune response-associated genes at Days 35-75. Immunohistochemical staining supported these observations by demonstrating an increase in CD4+, CD68+, and CD19+ cells in C57BL/6 but not CD40L-KO mice. Thus, the healthy host demonstrates a robust, biphasic response to infection by Pneumocystis; CD40L is an essential upstream regulator of the adaptive immune responses that efficiently control infection and prevent development of progressive pneumonia.
authors.affiliation: Critical Care Medicine Department, National Institutes of Health Clinical Center, NIH, Building 10, Room 2C145, MSC 1662, Bethesda, MD 20892-1662, USA.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Hernandez-Novoa
authors.forename: Lisa
authors.initials: L
authors.lastname: Bishop
authors.forename: Carolea
authors.initials: C
authors.lastname: Logun
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Munson
authors.forename: Eldad
authors.initials: E
authors.lastname: Elnekave
authors.forename: Zoila G
authors.initials: ZG
authors.lastname: Rangel
authors.forename: Jennifer
authors.initials: J
authors.lastname: Barb
authors.forename: Robert L
authors.initials: RL
authors.lastname: Danner
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Kovacs
mesh_terms: D000818:Animals; D023201:CD40 Ligand / Q000172:deficiency* / Q000235:genetics; D005260:Female; D015870:Gene Expression; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D011010:Pneumocystis / Q000276:immunology*; D016720:Pneumocystis Infections / Q000276:immunology*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D023201:CD40 Ligand