Analysis of human serum from women affected by cervical lesions.
Cervical cancer is one of the first causes of death in Mexican women population. The plasma proteome has a wide dynamic range concentrations of different protein and their alterations reflect the physiological state of the individual's health. The aim of this study was to characterize the 2D-PAGE serum patterns from healthy women and with different levels of cervical lesions. Changes in haptoglobin, apolipoproteins, and transthyretin, when comparing the serum from healthy women and serum from patients with different levels of cervical lesion were found. The Western blot analysis showed increasing concentrations of metalloproteinases (MMP's), proteins with important biological roles in tumor development and metastasis. Protein profiles in conjunction with MS, bioinformatics, and Western blot analysis, allow us to compile information for the acquisition of results to proposed candidates biomarkers of cervical cancer among Mexican women population.
Barba de la Rosa
Ana P
AP
División of Molecular Biology, Institute for Scientific and Technological Research in San Luis Potosi, Mexico. apbarba@ipicyt.edu.mx
Lugo-Melchor
Ofelia Y
OY
Briones-Cerecero
Erika P
EP
Chagolla-López
Alicia
A
De León-Rodríguez
Antonio
A
Santos
Leticia
L
Vázquez-Ortiz
Guelaguetza
G
Salcedo
Mauricio
M
D001053:Apolipoproteins / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood*; D015153:Blotting, Western; D016022:Case-Control Studies; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006242:Haptoglobins / Q000378:metabolism; D006801:Humans; D045726:Metalloproteases / Q000097:blood; D009361:Neoplasm Invasiveness; D011228:Prealbumin / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D002583:Uterine Cervical Neoplasms / Q000097:blood* / Q000473:pathology; D002578:Uterine Cervical Dysplasia / Q000097:blood / Q000473:pathology
D001053:Apolipoproteins; D014408:Biomarkers, Tumor; D006242:Haptoglobins; D011228:Prealbumin; D045726:Metalloproteases