Nucleic acid distribution pattern in avian erythrocytes and mammalian lymphocytes: comparative studies by fluorescence microscopy and digital imaging analytical techniques.
Nucleated erythrocytes of healthy domestic chicken and ducks, and lymphocytes of healthy Sprague Dawley rats were evaluated for nucleic acid distribution pattern, employing light and fluorescence microscopy procedures, as well as digital imaging analytical methods. The results demonstrate a unique organization of nuclear DNA of mature chicken and duck erythrocytes, as well as immature duck erythrocytes, as delineated spherical nuclear bodies that mostly corresponded with euchromatin zones of the cells in routine Wright-stain blood smears. The nuclear DNA of the rat lymphocytes, on the other hand, was observed as a more diffuse green fluorescing nuclear areas, with punctate variably-sized diffuse areas of RNA red fluorescence. RNA red color fluorescence was also evident in the narrow cytoplasm of the lymphocytes, especially in large lymphocytes, in comparison with the cytoplasm of the mature avian erythrocytes that completely lacked any nucleic acid fluorescence. Nuclear RNA fluorescence was lacking in the mature chicken erythrocytes, compared with those of the mature and immature duck erythrocytes as well as lymphocytes of both avian and rats blood. The significance of these findings lies in the establishment of normal benchmarks for the nuclear and cytoplasmic nucleic acid pattern in eukaryotic cells. These normal benchmarks become valuable in rapid diagnostic situations associated with pathologies, such as the presence of viral nuclear and cytoplasmic inclusion bodies that can alter the nucleic acid pattern of the host cells, and in conditions of cellular abnormal protein aggregations. Variability of cellular nucleic acid pattern can also aid in prognostic assessments of neoplastic conditions.
Isitor
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Department of Preclinical Sciences, The University of The West Indies, St. Augustine Campus, Trinidad, W.I., Trinidad and Tobago. isitorgn@yahoo.com
Asgarali
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Pouching
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D000165:Acridine Orange; D000818:Animals; D001717:Birds / Q000235:genetics*; D002467:Cell Nucleus / Q000502:physiology / Q000648:ultrastructure; D002645:Chickens; D004247:DNA / Q000032:analysis / Q000235:genetics*; D004372:Ducks; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000166:cytology / Q000502:physiology*; D018655:Lymphocyte Count; D008214:Lymphocytes / Q000166:cytology / Q000502:physiology*; D008322:Mammals / Q000235:genetics*; D008856:Microscopy, Fluorescence; D012313:RNA / Q000032:analysis / Q000235:genetics*
D012313:RNA; D004247:DNA; D000165:Acridine Orange