Quantitative phase microscopy through differential interference imaging.
An extension of Nomarski differential interference contrast microscopy enables isotropic linear phase imaging through the combination of phase shifting, two directions of shear, and Fourier space integration using a modified spiral phase transform. We apply this method to simulated and experimentally acquired images of partially absorptive test objects. A direct comparison of the computationally determined phase to the true object phase demonstrates the capabilities of the method. Simulation results predict and confirm results obtained from experimentally acquired images.
King
Sharon V
SV
University of Colorado, Department of Electrical and Computer Engineering, Campus Box 425 UCB Boulder, Colorado 80309-0425, USA. Sharon.king@colorado.edu
Libertun
Ariel
A
Piestun
Rafael
R
Cogswell
Carol J
CJ
Preza
Chrysanthe
C
D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*