title: Intestinal tumor progression is promoted by decreased apoptosis and dysregulated Wnt signaling in Ceacam1-/- mice.
abstract: The carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) is downregulated in colonic and intestinal hyperplastic lesions as well as in other cancers, where it functions as a tumor suppressor. To investigate the functions of CEACAM1 in the normal intestine and in intestinal tumors, we generated a compound knockout mouse model and examined both Ceacam1(-/-) and Apc(1638N/+):Ceacam1(-/-) mice. Ceacam1(-/-) intestinal cells exhibited a significant decrease in apoptosis, with no change in proliferation or migration, however. Compound Apc(1638N/+):Ceacam1(-/-) mice demonstrated an increase in intestinal tumor multiplicity and tumor progression. Increases in intussusceptions and desmoid lesions were also observed. We have shown that CEACAM1-L associates with beta-catenin by co-immunoprecipitation and colocalization in CEACAM1-L-transfected CT26 and CT51 mouse colon carcinoma cells. Ceacam1(-/-) enterocytes displayed decreased glycogen synthase kinase 3-beta activity with corresponding nuclear localization of beta-catenin. Increased T-cell factor/Lef transcriptional activity was observed in CEACAM1-null CT51 colonic cells and in Caco2 colon cancer cells in which CEACAM1 was downregulated. A significant increased expression in c-Myc and cyclin D1 targets of the Wnt signaling pathway was also revealed in the Ceacam1(-/-) intestine. CEACAM1 therefore actively participates in Wnt signaling in intestinal cells and its downregulation in intestinal tissue contributes to malignancy by augmenting tumor multiplicity and progression.
authors.affiliation: McGill Cancer Centre, McGill University, Montreal, Québec, Canada.
authors.forename: N
authors.initials: N
authors.lastname: Leung
authors.forename: C
authors.initials: C
authors.lastname: Turbide
authors.forename: B
authors.initials: B
authors.lastname: Balachandra
authors.forename: V
authors.initials: V
authors.lastname: Marcus
authors.forename: N
authors.initials: N
authors.lastname: Beauchemin
mesh_terms: D000818:Animals; D001483:Base Sequence; D002272:Carcinoembryonic Antigen / Q000235:genetics / Q000502:physiology*; D045744:Cell Line, Tumor; D017931:DNA Primers; D018450:Disease Progression; D007414:Intestinal Neoplasms / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D051153:Wnt Proteins / Q000378:metabolism*
chemical_list: D002272:Carcinoembryonic Antigen; C486183:Ceacam1 protein, mouse; D017931:DNA Primers; D051153:Wnt Proteins