Novel and selective potentiometric membrane sensor for amiloride determination in pharmaceutical compounds and urine.
A new PVC membrane sensor is described as a potentiometric sensor for amiloride. The sensor having amiloride-sodium tetraphenyl phthalate (ion-pair) as an electroactive material and dibutyl phthalate (DBP) as an anion excluder in PVC matrix in the percentage ratio of 4:66:30 (ion-pair: DBP:PVC) (w/w). The membrane sensor exhibits suitable response to amiloride in a concentration range of 1.0 x 10(-2) to 1.0 x 10(-6)mol L(-1) with a limit of detection of 9.9 x 10(-7)mol L(-1). The slope of the system was -54.3+/-1.0 mV decade(-1) over pH range of 2.0-7.0. Selectivity coefficients for amiloride relative to a numbers of potential interfering substances were investigated. The sensor was highly selective for amiloride over a large number of similar compounds. The sensor showing a fast response time of 6s and was used over a period of 2 months with a good reproducibility. The sensor was successfully applied to determination of amiloride in pharmaceutical samples with satisfactory results.
Ensafi
Ali A
AA
College of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran. Ensafi@cc.iut.ac.ir
Allafchian
Ali R
AR
D000584:Amiloride / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D015374:Biosensing Techniques / Q000379:methods*; D002138:Calibration; D004232:Diuretics / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D005215:Fasting; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017736:Ion-Selective Electrodes / Q000592:standards; D008567:Membranes, Artificial; D015394:Molecular Structure; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000737:chemistry / Q000652:urine; D011199:Potentiometry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D016482:Urinalysis / Q000379:methods*; D014867:Water / Q000737:chemistry
D004232:Diuretics; D008567:Membranes, Artificial; D004364:Pharmaceutical Preparations; D014867:Water; D000584:Amiloride