title: Effects of delays in peripheral blood processing, including cryopreservation, on detection of CD31 expression on naive CD4 T cells.
abstract: Delayed processing of peripheral blood or peripheral blood mononuclear cell isolation and cryopreservation can lead to the detection of somewhat higher levels of CD31 expression on naïve CD4 T cells by flow cytometry. These observations should be considered in the planning of multicenter clinical trials and in the interpretation of the results of functional studies.
authors.affiliation: AIDS Monitoring Laboratory, Clinical Services Program, SAIC-Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA.
authors.forename: Jeanette
authors.initials: J
authors.lastname: Higgins
authors.forename: Julia A
authors.initials: JA
authors.lastname: Metcalf
authors.forename: Randy A
authors.initials: RA
authors.lastname: Stevens
authors.forename: Michael
authors.initials: M
authors.lastname: Baseler
authors.forename: Michael
authors.initials: M
authors.lastname: Proschan
authors.forename: H Clifford
authors.initials: HC
authors.lastname: Lane
authors.forename: Irini
authors.initials: I
authors.lastname: Sereti
mesh_terms: D000328:Adult; D001793:Blood Preservation; D001800:Blood Specimen Collection; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D015925:Cryopreservation; D006679:HIV Seropositivity / Q000276:immunology* / Q000821:virology; D006801:Humans; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors
chemical_list: D019408:Platelet Endothelial Cell Adhesion Molecule-1