title: Analysis of Jembrana disease virus mRNA transcripts produced during acute infection demonstrates a complex transcription pattern.
abstract: Jembrana disease virus (JDV) is an unusual bovine lentivirus that causes an acute disease syndrome with a 20% case fatality rate after a short incubation period in Bos javanicus (Bali cattle) in Indonesia. Analysis of tat mRNA transcription patterns has identified up to six differently spliced transcripts indicating that, in common with other lentiviruses, JDV uses a complex splicing pattern. RT-PCR analysis of mRNA transcripts produced during the acute phase of infection with JDV(TAB/87) revealed at least 12 differently spliced transcripts involving 9 different splice sites. A single unspliced gag/pol transcript, singly spliced vif and tmx specific transcripts and alternatively spliced env, tat and rev transcripts were identified. A 67 nucleotide putative non-coding exon was identified that shared the same splice acceptor (SA) as vif and was incorporated into alternative transcripts of tat, rev and env.
authors.affiliation: School of Veterinary and Biomedical Science, Murdoch University, Murdoch, South Street, Perth, WA 6150, Australia.
authors.forename: Meredith E
authors.initials: ME
authors.lastname: Stewart
authors.forename: Moira
authors.initials: M
authors.lastname: Desport
authors.forename: Surachmi
authors.initials: S
authors.lastname: Setiyaningsih
authors.forename: Nining
authors.initials: N
authors.lastname: Hartaningsih
authors.forename: Graham E
authors.initials: GE
authors.lastname: Wilcox
mesh_terms: D000208:Acute Disease; D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002418:Cattle Diseases / Q000821:virology*; D015696:Gene Products, tat / Q000235:genetics / Q000378:metabolism; D016180:Lentivirus Infections / Q000662:veterinary* / Q000821:virology; D017960:Lentiviruses, Bovine / Q000145:classification / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D008969:Molecular Sequence Data; D022821:RNA Splice Sites; D012326:RNA Splicing; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D015696:Gene Products, tat; D022821:RNA Splice Sites; D012333:RNA, Messenger; D014764:Viral Proteins