Biocompatible ZnO/Au nanocomposites for ultrasensitive DNA detection using resonance Raman scattering.
A novel method for identifying DNA microarrays based on ZnO/Au nanocomposites functionalized with thiol-oligonucleotide as probes is descried here. DNA labeled with ZnO/Au nanocomposites has a strong Raman signal even without silver acting as a surface-enhanced Raman scattering promoter. X-ray photoelectron spectra confirmed the formation of a three-component sandwich assay, i.e., constituted DNA and ZnO/Au nanocomposites. The resonance multiple-phonon Raman signal of the ZnO/Au nanocomposites as a spectroscopic fingerprint is used to detect a target sequence of oligonucleotide. This method exhibits extraordinary sensitivity and the detection limit is at least 1 fM.
Liu
Yichun
Y
Centre for Advanced Optoelectronic Functional Materials Research, Institute of Genetics and Cytology, Northeast Normal University, Changchun, People's Republic of China. ycliu@nenu.edu.cn
Zhong
Mingya
M
Shan
Guiye
G
Li
Yajun
Y
Huang
Baiqu
B
Yang
Guoliang
G
D001483:Base Sequence; D004247:DNA / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D053761:Nanocomposites; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000379:methods*; D015034:Zinc Oxide / Q000737:chemistry*
D006046:Gold; D004247:DNA; D015034:Zinc Oxide