Taste novelty induces intracellular redistribution of NR2A and NR2B subunits of NMDA receptor in the insular cortex.
Taste recognition memory is a process by which animals associate a taste previously experienced with its gastric consequences. Novel taste presentation induces in the insular cortex biochemical modifications that decrease after the taste becomes familiar. Here we show that, in this cortex, consumption of a novel taste produces an increase of the NR2A and NR2B subunits of the NMDA receptor in the detergent resistant membrane (DRM) fraction. This increase did not occur in the adjacent parietal cortex, was not due to a change in the total amount of protein, and is related with the novelty of the stimulus since it was reduced after the taste became familiar. Furthermore, NR2A and NR2B subunits increase in the DRM was blocked by the injection of a muscarinic acetylcholine receptor antagonist. These results suggest that modulation of NMDA receptors in the insular cortex through the increase of its NR2A and NR2B subunits in the DRM is involved in the taste memory formation via a cholinergic process.
Núñez-Jaramillo
Luis
L
Departamento de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, A.P. 70-253 Mexico D.F., 04510, Mexico.
Jimenez
Beatriz
B
Ramirez-Munguía
Nadia
N
Delint-Ramírez
Ilse
I
Luna-Illades
Claudio
C
Tapia
Ricardo
R
Bermúdez-Rattoni
Federico
F
D000818:Animals; D002540:Cerebral Cortex / Q000378:metabolism*; D007425:Intracellular Membranes / Q000378:metabolism*; D008297:Male; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport; D051381:Rats; D017208:Rats, Wistar; D011976:Receptors, Muscarinic / Q000378:metabolism; D016194:Receptors, N-Methyl-D-Aspartate / Q000378:metabolism*; D021641:Recognition, Psychology / Q000502:physiology*; D013649:Taste / Q000502:physiology*
C120997:NR2A NMDA receptor; C121001:NR2B NMDA receptor; D021122:Protein Subunits; D011976:Receptors, Muscarinic; D016194:Receptors, N-Methyl-D-Aspartate