Globular adiponectin inhibits GnRH secretion from GT1-7 hypothalamic GnRH neurons by induction of hyperpolarization of membrane potential.
Reproduction is accurately regulated by metabolic states in mammals. Adiponectin regulates luteinizing hormone (LH) secretion in the pituitary and energy homeostasis in the hypothalamus. We further investigated the gonadotropin-releasing hormone (GnRH) secretion regulation by adiponectin and its related molecular and electrophysiological mechanisms. The results showed that adiponectin receptors (AdipR1 and 2) were expressed in GT1-7 cells derived from hypothalamus neurons. GnRH secretion was inhibited via activation of AMP-activated protein kinase (AMPK). Moreover, we revealed that hyperpolarization of plasma membrane potentials and reduction of calcium influx was also caused by adiponectin.
Wen
Jun-Ping
JP
Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrinology and Metabolism, Shanghai Key Laboratory of Endocrine Tumors, Endocrine and Metabolic Division, Shanghai Jiao Tong University School of Medicine, China.
Lv
Wen-Shan
WS
Yang
Jun
J
Nie
Ai-Fang
AF
Cheng
Xiao-Bing
XB
Yang
Ying
Y
Ge
Yan
Y
Li
Xiao-Ying
XY
Ning
Guang
G
D000091162:AMP-Activated Protein Kinase Kinases; D052242:Adiponectin / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004789:Enzyme Activation; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000378:metabolism
D052242:Adiponectin; D054419:Receptors, Adiponectin; C475419:adiponectin receptor 1, mouse; C475421:adiponectin receptor 2, mouse; D007987:Gonadotropin-Releasing Hormone; D011494:Protein Kinases; D000091162:AMP-Activated Protein Kinase Kinases; D002118:Calcium