Laboratory-scale production of lignin-degrading enzymes by free and entrapped cells ofPhanerochoete chrysosporium in a tubular air-lift bioreactor.
The production of lignin-degrading enzymes by free and entrapped cells ofPhanerochoete chrysosporium in a tubular air-lift bioreactor was studied. Under optimized cultural conditions the production of lignin peroxidase by free cells, calcium-alginate-entrapped cells and scouring-mesh-entrapped cells was in a ratio of 520ratio720ratio950 mU/mL, while the production of manganese peroxidase was in a ratio of 350ratio480ratio620 mU/mL. The stability of the entrapped cells by fed-batch systems was highest after 3 feeding experiments which is similarly demonstrated in the repeated use of the preparations in batch system.
Darah
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Fermentation and Enzyme Technology Laboratory, School of Biological Sciences, University Sains Malaysia, 11800 Minden, Penang, Malaysia.
Ibrahim
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