Acetylcholinesterase in intestinal cell differentiation involves G2/M cell cycle arrest.
Here we examine differentiation of the intestinal cell line Caco-2 following exposure to sodium butyrate (NaBT), using alkaline phosphatase (ALP) activity and carcinoembryonic antigen (CEA) levels as markers of differentiation. We show that acetylcholinesterase (AChE) activity and RNA levels increase during differentiation. Treatment with AChE inhibitors or knockdown of AChE levels by shRNA markedly decrease ALP and CEA levels in a concentration- and time-dependent manner. Finally, our observations suggest that NaBT-induced differentiation of intestinal cells involves AChE-induced cell cycle arrest.
Xiang
A C
AC
Laboratory of Molecular Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Shanghai, China.
Xie
J
J
Zhang
X J
XJ
D000110:Acetylcholinesterase / Q000235:genetics / Q000378:metabolism*; D000469:Alkaline Phosphatase / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002087:Butyrates / Q000378:metabolism; D018938:Caco-2 Cells; D002272:Carcinoembryonic Antigen / Q000378:metabolism; D002454:Cell Differentiation; D002455:Cell Division / Q000502:physiology*; D002800:Cholinesterase Inhibitors / Q000378:metabolism; D020895:Enterocytes / Q000166:cytology*; D016195:G2 Phase / Q000502:physiology*; D006801:Humans; D034622:RNA Interference
D015415:Biomarkers; D002087:Butyrates; D002272:Carcinoembryonic Antigen; D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase; D000469:Alkaline Phosphatase