Caenimonas koreensis gen. nov., sp. nov., isolated from activated sludge.
A Gram-negative, rod-shaped bacterium, designated strain EMB320T, was isolated from activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor. The isolate was strictly aerobic and non-motile. Growth was observed between 10 and 35 degrees C (optimum 30 degrees C) and between pH 6.0 and 9.0 (optimum pH 7.0-8.0). The predominant cellular fatty acids of strain EMB320T were C16 : 0, C18 : 1omega7c and summed feature 3 (C16 : 1omega7c and/or iso-C15 : 0 2-OH). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Strain EMB320T contained ubiquinone-8 (Q-8) as the major respiratory quinone system and 2-hydroxyputrescine and putrescine as the major polyamines, which suggests that it belongs to the Betaproteobacteria. The G+C content of the genomic DNA was 62.7 mol%. Comparative 16S rRNA gene sequence analysis showed that strain EMB320T formed a phyletic lineage distinct from other genera within the family Comamonadaceae. On the basis of chemotaxonomic data and molecular properties, strain EMB320T represents a novel genus and species within the family Comamonadaceae, for which the name Caenimonas koreensis sp. nov. is proposed. The type strain of Caenimonas koreensis is EMB320T (=KCTC 12616T =DSM 17982T).
Ryu
Seung Hyun
SH
Division of Applied Life Science (BK21 Program), EB-NCRC, PMBBRC, Gyeongsang National University, Jinju 660-701, Republic of Korea.
Lee
Dae Sung
DS
Park
Minjeong
M
Wang
Qian
Q
Jang
Ho Hee
HH
Park
Woojun
W
Jeon
Che Ok
CO
D015373:Bacterial Typing Techniques; D019149:Bioreactors; D042621:Comamonadaceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D004269:DNA, Bacterial / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D010758:Phosphorus / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D013045:Species Specificity
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D010758:Phosphorus