title: NO dioxygenase activity in hemoglobins is ubiquitous in vitro, but limited by reduction in vivo.
abstract: Genomics has produced hundreds of new hemoglobin sequences with examples in nearly every living organism. Structural and biochemical characterizations of many recombinant proteins reveal reactions, like oxygen binding and NO dioxygenation, that appear general to the hemoglobin superfamily regardless of whether they are related to physiological function. Despite considerable attention to "hexacoordinate" hemoglobins, which are found in nearly every plant and animal, no clear physiological role(s) has been assigned to them in any species. One popular and relevant hypothesis for their function is protection against NO. Here we have tested a comprehensive representation of hexacoordinate hemoglobins from plants (rice hemoglobin), animals (neuroglobin and cytoglobin), and bacteria (Synechocystis hemoglobin) for their abilities to scavenge NO compared to myoglobin. Our experiments include in vitro comparisons of NO dioxygenation, ferric NO binding, NO-induced reduction, NO scavenging with an artificial reduction system, and the ability to substitute for a known NO scavenger (flavohemoglobin) in E. coli. We conclude that none of these tests reveal any distinguishing predisposition toward a role in NO scavenging for the hxHbs, but that any hemoglobin could likely serve this role in the presence of a mechanism for heme iron re-reduction. Hence, future research to test the role of Hbs in NO scavenging would benefit more from the identification of cognate reductases than from in vitro analysis of NO and O(2) binding.
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, Iowa, United States of America.
authors.forename: Benoit J
authors.initials: BJ
authors.lastname: Smagghe
authors.forename: James T
authors.initials: JT
authors.lastname: Trent
authors.forename: Mark S
authors.initials: MS
authors.lastname: Hargrove
mesh_terms: D000818:Animals; D002384:Catalysis; D004926:Escherichia coli; D005288:Ferredoxins / Q000378:metabolism; D016166:Free Radical Scavengers / Q000378:metabolism; D006418:Heme / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism*; D006736:Horses; D006801:Humans; D007501:Iron / Q000378:metabolism; D007700:Kinetics; D009154:Mutation; D009211:Myoglobin / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D012275:Oryza / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism; D010100:Oxygen / Q000378:metabolism; D010105:Oxygenases / Q000378:metabolism*; D013057:Spectrum Analysis; D046939:Synechocystis / Q000378:metabolism
chemical_list: D005288:Ferredoxins; D016166:Free Radical Scavengers; D006454:Hemoglobins; D009211:Myoglobin; D009569:Nitric Oxide; D006418:Heme; D007501:Iron; D010088:Oxidoreductases; D010105:Oxygenases; C114415:nitric oxide dioxygenase; D010100:Oxygen