Competitive reaction kinetics of sulfate-reducing bacteria and methanogenic bacteria in anaerobic filters.
A kinetic model for the anaerobic filter (AF) that takes into account the mass fractions of sulfate-reducing bacteria (SRB) (fSRB) and methanogenic bacteria (MB) (fMB) and an inhibiting effect of H2S on bacterial groups is proposed. When the acetate-fed AFs were maintained at the low organic loading rate of 2.5kg COD/m3d, variations of the influent COD/SO4(2-) ratio (0.5-3.0) does not materially affect the acetate removal efficiency (all varying between 98.1% and 99.7%). With an increase in influent COD/SO4(2-) ratio, both the biofilm thickness and the specific substrate utilization rate decreased slightly but f(SRB) decreased markedly. The estimated results of fSRB and fMB showed that SRB out-competed MB for bacterial growth if the influent COD/SO4(2-) ratio was maintained at less than 1.3, whereas MB out-competed SRB for bacterial growth if the influent COD/SO4(2-) ratio was maintained at greater than 2.0. The specific substrate utilization rate of SRB (0.19-0.24mg acetate/mg VSSd) was lower than that of MB (0.31-0.59mg acetate/mg VSSd). The estimated kinetic parameters disclosed that the affinity of acetate to MB was higher and unionized H2S imposed a greater inhibiting effect on MB. The model simulation results (acetate and sulfate removal) agreed well with the experimental results.
Chou
Hsin-Hsien
HH
Department of Environmental Engineering, Kun Shan University, 949 Da-Wan Road, Yun Kan, Tainan County 710, Taiwan, ROC. envi1234@mail.ksu.edu.tw
Huang
Ju-Sheng
JS
Chen
Wen-Guang
WG
Ohara
Reiko
R
D000085:Acetates / Q000032:analysis; D000693:Anaerobiosis; D018441:Biofilms; D003198:Computer Simulation; D005374:Filtration; D007700:Kinetics; D008697:Methane / Q000378:metabolism*; D008954:Models, Biological; D015203:Reproducibility of Results; D013431:Sulfates / Q000032:analysis; D016999:Sulfur-Reducing Bacteria / Q000254:growth & development*
D000085:Acetates; D013431:Sulfates; D008697:Methane