title: Aldo-keto reductase from Helicobacter pylori--role in adaptation to growth at acid pH.
abstract: Pyridine-linked oxidoreductase enzymes of Helicobacter pylori have been implicated in the pathogenesis of gastric disease. Previous studies in this laboratory examined a cinnamyl alcohol dehydrogenase that was capable of detoxifying a range of aromatic aldehydes. In the present work, we have extended these studies to identify and characterize an aldoketo reductase (AKR) enzyme present in H. pylori. The gene encoding this AKR was identified in the sequenced strain of H. pylori, 26695. The gene, referred to as HpAKR, was cloned and expressed in Escherichia coli as a His-tag fusion protein, and purified using nickel chelate chromatography. The gene product (HpAKR) has been assigned to the AKR13C1 family, although it differs in specificity from the two other known members of this family. The enzyme is a monomer with a molecular mass of approximately 39 kDa on SDS/PAGE. It reduces a range of aromatic aldehyde substrates with high catalytic efficiency, and exhibits dual cofactor specificity for both NADPH and NADH. HpAKR can function over a broad pH range (pH 4-9), and has a pH optimum of 5.5. It is inhibited by sodium valproate. Its substrate specificity complements that of the cinnamyl alcohol dehydrogenase activity in H. pylori, giving the organism the capacity to reduce a wide range of aldehydes. Generation of an HpAKR isogenic mutant of H. pylori demonstrated that HpAKR is required for growth under acidic conditions, suggesting an important role for this enzyme in adaptation to growth in the gastric mucosa. This AKR is a member of a hitherto little-studied class.
authors.affiliation: School of Food Science and Environmental Health, Dublin Institute of Technology, Ireland.
authors.forename: Denise
authors.initials: D
authors.lastname: Cornally
authors.forename: Blanaid
authors.initials: B
authors.lastname: Mee
authors.forename: Ciarán
authors.initials: C
authors.lastname: MacDonaill
authors.forename: Keith F
authors.initials: KF
authors.lastname: Tipton
authors.forename: Dermot
authors.initials: D
authors.lastname: Kelleher
authors.forename: Henry J
authors.initials: HJ
authors.lastname: Windle
authors.forename: Gary T M
authors.initials: GT
authors.lastname: Henehan
mesh_terms: D000222:Adaptation, Physiological; D000429:Alcohol Oxidoreductases / Q000737:chemistry* / Q000235:genetics; D000449:Aldehyde Reductase; D000074408:Aldo-Keto Reductases; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D002384:Catalysis; D004795:Enzyme Stability; D016480:Helicobacter pylori / Q000201:enzymology* / Q000254:growth & development; D006863:Hydrogen-Ion Concentration; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins / Q000378:metabolism; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D000429:Alcohol Oxidoreductases; D000074408:Aldo-Keto Reductases; D000449:Aldehyde Reductase