title: A global modulatory role for the Yersinia enterocolitica H-NS protein.
abstract: The H-NS protein plays a significant role in the modulation of gene expression in Gram-negative bacteria. Whereas isolation and characterization of hns mutants in Escherichia coli, Salmonella and Shigella represented critical steps to gain insight into the modulatory role of H-NS, it has hitherto not been possible to isolate hns mutants in Yersinia. The hns mutation is considered to be deleterious in this genus. To study the modulatory role of H-NS in Yersinia we circumvented hns lethality by expressing in Y. enterocolitica a truncated H-NS protein known to exhibit anti-H-NS activity in E. coli (H-NST(EPEC)). Y. enterocolitica cells expressing H-NST(EPEC) showed an altered growth rate and several differences in the protein expression pattern, including the ProV protein, which is modulated by H-NS in other enteric bacteria. To further confirm that H-NST(EPEC) expression in Yersinia can be used to demonstrate H-NS-dependent regulation in this genus, we used this approach to show that H-NS modulates expression of the YmoA protein.
authors.affiliation: Institut de Bioenginyeria de Catalunya, Parc Científic de Barcelona, Edifici Hèlix. c/ Josep Samitier 1-5, 08028 Barcelona, Spain.
authors.forename: Rosa C
authors.initials: RC
authors.lastname: Baños
authors.affiliation: Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain.
authors.forename: José I
authors.initials: JI
authors.lastname: Pons
authors.affiliation: Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Madrid
authors.affiliation: Departament de Microbiologia, Facultat de Biologia, Universitat de Barcelona, Avda Diagonal 645, 08028 Barcelona, Spain.|Institut de Bioenginyeria de Catalunya, Parc Científic de Barcelona, Edifici Hèlix. c/ Josep Samitier 1-5, 08028 Barcelona, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Juárez
mesh_terms: D001426:Bacterial Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D020043:Genes, Essential; D020543:Proteome / Q000032:analysis; D012329:RNA, Bacterial / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017384:Sequence Deletion; D015008:Yersinia enterocolitica / Q000737:chemistry / Q000235:genetics / Q000254:growth & development / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C031908:H-NS protein, bacteria; D020543:Proteome; D012329:RNA, Bacterial; D012333:RNA, Messenger; C079210:YmoA protein, Yersinia enterocolitica