Ultrabroadband multiplex CARS microspectroscopy and imaging using a subnanosecond supercontinuum light source in the deep near infrared.
Subnanosecond supercontinuum (SC) has been generated by a 1,064 nm microchip laser combined with a photonic crystal fiber. The ultrabroadband (>2,000 cm(-1)) SC has facilitated multiplex coherent anti-Stokes Raman scattering (CARS) microspectroscopy in the spectral range from 1,000 to 3,000 cm(-1) with lateral and depth spatial resolution of 0.9 and 4.6 microm, respectively. A clear CARS image of a Nicotiana tabacum L. cv. Bright Yellow 2 cell has been obtained with high vibrational contrast.
Okuno
Masanari
M
Department of Chemistry, School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo, Tokyo 113-0033, Japan.
Kano
Hideaki
H
Leproux
Philippe
P
Couderc
Vincent
V
Hamaguchi
Hiro-O
HO
D007259:Infrared Rays; D007368:Interferometry / Q000379:methods; D008027:Light; D008853:Microscopy / Q000379:methods; D013059:Spectrum Analysis, Raman; D014026:Nicotiana / Q000166:cytology*