title: Effects of Dyract AP and released ionic products on periodontal ligament cells and bone marrow cultures.
abstract: OBJECTIVE
  The aim of this work was to investigate the release of inorganic ionic products from specimens of the polyacid-modified composite resin Dyract AP (DAP) and furthermore, to analyze the biological effect of DAP and the medium extract in human periodontal ligament (PDL) cells and mouse bone marrow cell (BMC) cultures.
  
  
  METHODS
  Ion release from DAP specimens immersed in cell culture medium was analyzed with inductively coupled plasma optical emission spectroscopy (ICP-OES). Cells were cultured with either DAP specimens or with DAP media extract and effects on cell proliferation, osteoblastic gene expression and mineralization capacity were analyzed with direct-contact tests, neutral red (NR) uptake, quantitative real-time PCR and a bone nodule formation assay.
  
  
  RESULTS
  ICP-OES analysis of DAP extract demonstrated a significant increase in fluoride, strontium and silica. PDL cells demonstrated normal growth pattern in the direct-contact tests with the material. DAP extracts produced a dose-dependent stimulation of cell proliferation and concomitant inhibition of osteoblast specific markers and nodule formation.
  
  
  CONCLUSIONS
  The compomer may have possible bioactive properties due to ions leaching out from the filler component.
authors.affiliation: Department of Odontology, Medical Faculty, Umeå University, Sweden.
authors.forename: Annika
authors.initials: A
authors.lastname: Sahlin-Platt
authors.forename: Ulf
authors.initials: U
authors.lastname: Ortengren
authors.forename: Zivko
authors.initials: Z
authors.lastname: Mladenovic
authors.forename: Maria
authors.initials: M
authors.lastname: Ransjö
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000187:drug effects*; D002113:Calcification, Physiologic / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D020770:Compomers / Q000494:pharmacology*; D005461:Fluorine / Q000032:analysis; D006801:Humans; D007477:Ions / Q000032:analysis; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology / Q000187:drug effects*; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D013053:Spectrophotometry / Q000379:methods; D013324:Strontium / Q000032:analysis
chemical_list: D020770:Compomers; C088486:Dyract; D007477:Ions; D012387:Root Canal Filling Materials; D005461:Fluorine; D013324:Strontium