Dietary phosphatidylcholine affects postprandial plasma levels and digestibility of lipid in common carp (Cyprinus carpio).
This study examined the effect of purified soyabean phosphatidylcholine (SPC) on circulating plasma lipids and nutrient digestibility in juvenile common carp. The fish (100 (SD 15) g, 25 degrees C) were fed, for 4 weeks, a casein-based diet containing either 12 % soyabean oil (diet SBO) or 8 % SBO plus 4 % SPC (diet SPC). The lipid, protein and energy contents of the faeces were analysed for the determination of apparent digestibility. At the end of the trial, the fish were fasted for 48 h and fed a single meal. Plasma lipids were then analysed over the next 48 h. The growth (1.63 v. 1.26 % per d) and apparent lipid digestibility (96.3 v. 92.1 %) were higher in SPC- than in SBO-fed fish. The amplitude of the postprandial (8 h after the meal) TAG peak was identical in fish from both treatments, despite the 33 % lower amount of TAG in diet SPC. Both observations support the idea that SPC stimulates intestinal TAG uptakes. The lower TAG:phospholipid ratio of the secreted plasma lipids at the time of absorption suggests a larger number of smaller intestinal lipoproteins in SPC- than SBO-fed fish, possibly due to the recycling of absorbed lysophosphatidylcholine for chylomicron formation. In the 48 h unfed state, phospholipid levels remained approximately 20 % higher in SPC-fed than in SBO-fed fish, but we observed no hypocholesterolaemic effect of SPC. In summary, the present data support earlier histological indications of a positive role of dietary phosphatidylcholine in intestinal TAG uptakes in carp.
Geurden
Inge
I
INRA, UMR1067 Nutrition, Aquaculture and Genomics, NuAGe, INRA Hydrobiology Station, Saint Pée-sur-Nivelle F-64310, France.
Kaushik
Sadasivam
S
Corraze
Geneviève
G
D000821:Animal Feed; D000818:Animals; D002347:Carps / Q000254:growth & development / Q000378:metabolism*; D002364:Caseins / Q000008:administration & dosage / Q000378:metabolism; D004063:Digestion; D005243:Feces / Q000737:chemistry; D050356:Lipid Metabolism; D008055:Lipids / Q000097:blood*; D010713:Phosphatidylcholines / Q000008:administration & dosage*; D019518:Postprandial Period; D013024:Soybean Oil / Q000008:administration & dosage / Q000378:metabolism
D002364:Caseins; D008055:Lipids; D010713:Phosphatidylcholines; D013024:Soybean Oil