[Construction and targeting study of eukaryotic expression vector modulated by a macrophage-specific promoter].
OBJECTIVE
To construct a macrophage-specific eukaryotic expression vector and to investigate its expressing specificity.


METHODS
Macrophage-specific promoter was synthesized using PCR, and substituted the CMV promoter of eukaryotic expression vector pEGFP-N1 to construct a recombinant vector (pSP-GFP), which were cotransfected with pERFP-N1 vector into different cell lines. The green fluorescent protein (GFP) and red fluorescent protein (RFP) was observed by fluorescence microscopy, and the target specificity of macrophage-specific promoter was judged by comparison of expressed level of GFP and RFP in various cell lines.


RESULTS
The macrophage-specific eukaryotic expression vector was successfully constructed, which showed strong activity and specificity only in macrophage cells.


CONCLUSIONS
The constructed pSP-GFP vector was macrophage-specific, which are potential to targeted gene therapy of intracellular bacterial infection.
Wen
Yang-an
YA
Faculty of Laboratory Medicine, Chongqing Medical University, the Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Chongqing 400016, China. wenyangan0105@yahoo.com.cn
Guo
Jin-ying
JY
Yu
Xiao-lin
XL
Cen
Dong
D
Liu
Jin-bo
JB
Chen
Yan
Y
Zhang
Jian
J
Tu
Zhi-guang
ZG
D004742:Enhancer Elements, Genetic; D056890:Eukaryota / Q000235:genetics*; D015316:Genetic Therapy; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008264:Macrophages / Q000502:physiology*; D008297:Male; D011401:Promoter Regions, Genetic / Q000235:genetics* / Q000502:physiology; D019098:Telomerase / Q000378:metabolism; D014162:Transfection; D019444:Vaccines, DNA / Q000008:administration & dosage; D037521:Virulence Factors / Q000235:genetics
D019444:Vaccines, DNA; D037521:Virulence Factors; D049452:Green Fluorescent Proteins; D019098:Telomerase