Control of oral malodour by dentifrices measured by gas chromatography.
OBJECTIVE
To evaluate the effect of toothpaste treatments on levels of oral volatile sulphur compounds (VSCs) measured by gas chromatography in two clinical studies.


METHODS
These were blinded, randomised, controlled, crossover studies with 16 (study A) or 20 (study B) healthy volunteers between the ages of 19-54. Study A: breath samples were collected at baseline, immediately and lhr after brushing. Four dentifrices (Zinc A, Zinc B, commercially available triclosan dentifrice and zinc free control) were evaluated. Study B: breath samples were collected at baseline, immediately, 1, 2, 3 and 7 hours after treatment. Subjects consumed a light breakfast then provided an additional breath sample between baseline assessment and treatment. Two dentifrices (gel-to-foam and a commercially available triclosan dentrifrice) were evaluated. Breath samples were collected in syringes and analysed for VSCs (hydrogen sulphide, methyl mercaptan and Total VSCs) utilising gas chromatography (GC) with flame photometric detection.


RESULTS
Study A: immediately after treatment, a statistically significant reduction in VSCs from baseline was observed for Zinc A product only. A statistically significant reduction in VSCs from baseline was observed after 1 hour for all products. Both zinc products exhibited a significantly greater reduction from baseline VSCs than Colgate Total and Control at all time points. Study B: a statistically significant reduction in VSCs from baseline was observed at all time points for both products. The gel-to-foam product exhibited significantly greater reduction from baseline Total VSC concentration than Colgate Total at all time points from 1 hour post-treatment.


CONCLUSIONS
Control of oral malodour by toothpaste treatment, evaluated as VSC levels using GC, has been demonstrated. Zinc is effective at reducing VSCs and the efficacy of zinc is formulation dependent. A gel-to-foam dentifrice was more effective at reducing VSCs than Colgate Total up to 7 hours.
Newby
Evelyn E
EE
GlaxoSmithKline Consumer Healthcare, Weybridge, UK. Evelyn.E.Newby@gsk.com
Hickling
Jenneth M
JM
Hughes
Francis J
FJ
Proskin
Howard M
HM
Bosma
Marylynn P
MP
D000328:Adult; D002849:Chromatography, Gas / Q000379:methods; D003802:Dentifrices / Q000737:chemistry / Q000627:therapeutic use*; D004812:Epidemiologic Methods; D005260:Female; D006209:Halitosis / Q000517:prevention & control*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013997:Time Factors; D014099:Toothbrushing / Q000379:methods; D016896:Treatment Outcome
D003802:Dentifrices