Transcriptional auto-regulation of the dopamine receptor regulating factor (DRRF) gene.
The murine dopamine receptor regulating factor (DRRF) gene is transcribed from a TATA-less promoter that has several putative Sp1 binding sites. The present investigation identifies functional transcription factors that modulate the expression of this gene. In the D2-dopamine receptor expressing NB41A3 cells, Sp1 potently activates transcription from the DRRF promoter in pCAT-DRRF-1153/+17, while DRRF itself effectively inhibits it. Sp1 also activates this promoter in pCAT-DRRF-310/+17. In competitive co-transfection experiments, DRRF represses the transcriptional activation induced by Sp1, while Sp1 de-represses the inhibitory effect of DRRF. Deletion of the 31 bp fragment between -1153 and -1122 decreased basal transcription down to about 60%. This fragment contains a functional AP1 binding site. In addition, deletion of the 129 bp region between -901 and -772 further decreased transcription. The latter region has a functional AP2 binding site. The present observations suggest that DRRF auto-regulates its own promoter by competing with Sp1 and that both AP1 and AP2 modulate expression of this gene.
Lee
Sang-Hyeon
SH
Department of Bioscience and Biotechnology, Silla University, San 1-1, Kwaebop-dong, Sasang-gu, Busan 617-736, Republic of Korea. slee@silla.ac.kr
Jang
Min-Kyung
MK
Lee
Ok Hee
OH
Kim
Ok Soo
OS
Kim
Yong-Man
YM
Yajima
Shunsuke
S
Lee
Young-Choon
YC
Mouradian
M Maral
MM
D000818:Animals; D045744:Cell Line, Tumor; D051741:Kruppel-Like Transcription Factors / Q000235:genetics*; D051379:Mice; D011401:Promoter Regions, Genetic; D017384:Sequence Deletion; D016329:Sp1 Transcription Factor / Q000235:genetics; D018808:Transcription Factor AP-1 / Q000235:genetics; D050656:Transcription Factor AP-2 / Q000235:genetics; D024363:Transcription Initiation Site; D014158:Transcription, Genetic; D015533:Transcriptional Activation
C486609:Klf16 protein, mouse; D051741:Kruppel-Like Transcription Factors; D016329:Sp1 Transcription Factor; D018808:Transcription Factor AP-1; D050656:Transcription Factor AP-2