title: Quantitative determination of singlet oxygen generated by excited state aromatic amino acids, proteins, and immunoglobulins.
abstract: Singlet oxygen quantum yields generated by excited state aromatic amino acids (tryptophan, tyrosine, phenylalanine), N-acetylated amino acids (N-acetyl-tryptophan, N-acetyl-tyrosine, N-acetyl-phenylalanine), and from selected proteins and immunoglobulins have been quantified by time-resolved phosphorescence measurements. A small, but significant, quantum yield found for proteins and immunoglobulins demonstrates that molecular oxygen can diffuse through the polypeptide matrix and can be sensitized by residues buried within the folds of protein structure.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA.
authors.forename: Khin K
authors.initials: KK
authors.lastname: Chin
authors.forename: Colleen C
authors.initials: CC
authors.lastname: Trevithick-Sutton
authors.forename: Jeremy
authors.initials: J
authors.lastname: McCallum
authors.forename: Steffen
authors.initials: S
authors.lastname: Jockusch
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Turro
authors.forename: J C
authors.initials: JC
authors.lastname: Scaiano
authors.forename: Christopher S
authors.initials: CS
authors.lastname: Foote
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Garcia-Garibay
mesh_terms: D000418:Albumins / Q000737:chemistry; D024322:Amino Acids, Aromatic / Q000737:chemistry*; D007136:Immunoglobulins / Q000737:chemistry*; D008163:Luminescent Measurements; D010047:Ovalbumin / Q000737:chemistry; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry*; D026082:Singlet Oxygen / Q000032:analysis / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
chemical_list: D000418:Albumins; D024322:Amino Acids, Aromatic; D007136:Immunoglobulins; D011506:Proteins; D026082:Singlet Oxygen; D010047:Ovalbumin