Ultraviolet-A irradiation but not ultraviolet-B or infrared-A irradiation leads to a disturbed zinc homeostasis in cells.
Changes of the redox balance in cells alter the availability of intracellular free Zn(2+). Here, cells were exposed to ultraviolet (UV)-A, UV-B, or infrared (IR)-A light irradiation, and the intracellular free zinc pool was monitored. Under sublethal conditions only UV-A irradiation resulted in a transient cytoplasmic and nuclear increase of intracellular free Zn(2+). Likewise, tert-butyl hydroperoxide and singlet oxygen, but not H(2)O(2) or intracellular generation of O(2)(*-) by redox cyclers, mimicked the effects of UV-A irradiation, while disulfide stress by diamide only led to a transient cytoplasmic zinc release. These results show that only certain types of subtoxic cellular stress massively disturb the zinc homeostasis in cells.
Pirev
Elvis
E
Institute of Biochemistry and Molecular Biology I, Heinrich-Heine-University of Düsseldorf, D-40225 Düsseldorf, Germany.
Calles
Christian
C
Schroeder
Peter
P
Sies
Helmut
H
Kröncke
Klaus-D
KD
D000818:Animals; D002460:Cell Line; D003958:Diamide / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D006706:Homeostasis / Q000187:drug effects / Q000528:radiation effects*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D010084:Oxidation-Reduction; D011809:Quinones / Q000378:metabolism; D026082:Singlet Oxygen / Q000378:metabolism; D014466:Ultraviolet Rays; D015032:Zinc / Q000378:metabolism*
D011809:Quinones; D003958:Diamide; D026082:Singlet Oxygen; D006861:Hydrogen Peroxide; D015032:Zinc