Dietary cooked navy beans and their fractions attenuate colon carcinogenesis in azoxymethane-induced ob/ob mice.
Based on the protective effects of cooked dry bean consumption in a human intervention study, we evaluated which fraction of cooked dry beans is responsible for its cancer-preventive effects. Cooked navy beans (whole beans), the insoluble fraction (bean residue) or soluble fraction of the 60% (vol:vol) ethanol extract of cooked navy beans (bean extract), or a modified AIN-93G diet (16.6% fat including 12.9% lard) as control diet were fed to 160 male obese ob/ob mice after 2 azoxymethane injections. In comparison to control-fed mice, dysplasia, adenomas, or adenocarcinomas were detected in fewer mice on either bean fraction diet (percent reduction from control: whole beans 54%, P=0.10; bean residue 81%, P=0.003; bean extract 91%, P=0.007), and any type of colon lesions, including focal hyperplasia, were found in fewer mice on each of the 3 bean diets percent reduction from control: whole bean 56%, P=0.04; bean residue 67%, P=0.01; bean extract 87%, P=0.0003. These results suggest that both the soluble and the insoluble fraction of the extract contribute to the cancer-protective effect of cooked navy beans.
Bobe
Gerd
G
Laboratory of Cancer Prevention, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, Maryland 21702, USA. bobeg@mail.nih.gov
Barrett
Kathleen G
KG
Mentor-Marcel
Roycelynn A
RA
Saffiotti
Umberto
U
Young
Matthew R
MR
Colburn
Nancy H
NH
Albert
Paul S
PS
Bennink
Maurice R
MR
Lanza
Elaine
E
D000230:Adenocarcinoma / Q000139:chemically induced / Q000517:prevention & control; D000236:Adenoma / Q000139:chemically induced / Q000517:prevention & control; D000818:Animals; D016588:Anticarcinogenic Agents / Q000032:analysis / Q000494:pharmacology*; D001397:Azoxymethane / Q000037:antagonists & inhibitors* / Q000633:toxicity; D002273:Carcinogens / Q000037:antagonists & inhibitors / Q000633:toxicity; D003110:Colonic Neoplasms / Q000139:chemically induced / Q000517:prevention & control*; D007887:Fabaceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D008820:Mice, Obese; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology*; D011897:Random Allocation; D012995:Solubility
D016588:Anticarcinogenic Agents; D002273:Carcinogens; D010936:Plant Extracts; D001397:Azoxymethane