Restricted quasispecies variation following infection with the GB virus B.
The extent of genetic variability following acute infection of tamarins with GB virus B (GBV-B) is not known. In this study we attempted to define the quasispecies variation of GBV-B 17 days post-infection, by PCR amplification of GBV-B RNA extracted from serum and liver. Cloning followed by sequencing revealed a small number of changes in the three regions studied, namely the 5' untranslated region, E2 and NS3. Moreover, there was no region of high amino acid variability in E2, akin to hypervariable region 1 of hepatitis C virus. This was further confirmed by analysing sequences from two additional animals obtained at a similar time point post-infection. Nevertheless, it was apparent that different variants with one or two amino acid substitutions in the region studied had been selected when comparing the sequences from the three animals. This restricted sequence variation of GBV-B during acute hepatitis may explain the infrequent progression of the infection to a chronic stage.
McGarvey
Michael Joseph
MJ
Department of Medicine, Imperial College, Faculty of Medicine, St. Mary's Campus, London, United Kingdom.
Iqbal
Mohammed
M
Nastos
Theodoros
T
Karayiannis
Peter
P
D020121:5' Untranslated Regions / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018178:Flaviviridae Infections / Q000821:virology*; D029406:GB virus B / Q000737:chemistry / Q000235:genetics*; D014644:Genetic Variation; D006524:Hepatitis, Viral, Animal / Q000821:virology*; D051039:Leontopithecus; D023421:Models, Animal; D008969:Molecular Sequence Data; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
D020121:5' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins