[Selection of genes related to multidrug resistance of pancreatic ductal adenocarcinoma by microarray analysis].
OBJECTIVE
To investigate the genes concerning multidrug resistance (MDR) of pancreatic ductal adenocarcinoma with microarray analysis.


METHODS
Gene expression profile of pancreatic cancer cell line SW1990 and resistance subline SW1990/5-FU, SW1990/ADM, SW1990/GEM were screened in two independent replicates using oligonucleotide microarray (Affymetrix HG U133 2.0 plus) which contained 38,500 human genes. And advanced bioinformatics analysis was conducted.


RESULTS
Totally, 165 genes and expressed sequence tags (ESTs), which were seldom reported to be related with drug resistance before,were statistically difference and the fold change was up- or down-regulated at least 2 folds in all 3 resistant sub-lines when compared with SW1990. According gene ontology, the genes related to oxidoreductase activity, apoptosis, cell cycle, signal transduction and cell adhesion might be some epigenetic changes for MDR development. Hierarchical clustering analysis, showed several interesting clusters, namely, TNKS2, PRDX4 and CCDC4.


CONCLUSIONS
MDR of pancreatic cancer is a complicated and multifactorial process. In the present study, a widespread differential gene expression pattern was constructed in PDAC multidrug resistant cells. Advanced study will provide new targets for MDR research and cast insights into research of the molecular mechanism of MDR.
Feng
Bin
B
Department of Surgery, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing, China.
Zhao
Yu-pei
YP
Chen
Ge
G
Zhang
Tai-ping
TP
Wu
Yuan-de
YD
D000230:Adenocarcinoma / Q000235:genetics / Q000473:pathology; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics / Q000473:pathology; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D018432:Drug Resistance, Multiple / Q000235:genetics*; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D004354:Drug Screening Assays, Antitumor; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000473:pathology