title: Zinc-containing phosphate-based glasses for tissue engineering.
abstract: It has been shown that the addition of zinc to hydroxyapatite promotes osteoblast cell adhesion (Ishaug et al 1994 J. Biomed. Mater. Res. A 28 1445-53). Therefore, this study presents a series of phosphate-based glass compositions that contain varying amounts of zinc in order to promote osteoblast cell adhesion. The compositions investigated were P(50)Ca((40-x))Na(10) where x = 0, 1, 2, 3, 4 and 5 mol%. The dissolution rate and effect on pH of glass discs were investigated and ion release from the glass discs was examined in distilled water at 37 degrees C after 1, 2, 3, 4 and 7 days. The attachment of osteoblast-like cells (HOB) was observed by SEM on the glass discs. A linear decrease in the %mass of the glass discs was observed for all compositions for the duration of the study. The dissolution rates were similar for all the compositions. After 7 days, a mass loss of 3-6% was observed for all the compositions. The pH of distilled water decreased to a range of pH 4.5-5.5 from pH 7 after 1 day, and remained in this range for the duration of the study. The composition containing 0 mol% Zn reduced the pH to a lesser extent than the composition containing the highest amount of Zn. Furthermore, Zn(2+) ion release was observed from all the Zn-containing compositions. These glass compositions may therefore be suitable for tissue engineering applications.
authors.affiliation: Division of Biomaterials and Tissue Engineering, UCL Eastman Dental Institute, 256 Gray's Inn Road, London, WC1X 8LD, UK.
authors.forename: V
authors.initials: V
authors.lastname: Salih
authors.forename: A
authors.initials: A
authors.lastname: Patel
authors.forename: J C
authors.initials: JC
authors.lastname: Knowles
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion / Q000502:physiology; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D017886:Durapatite / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D016348:Osseointegration / Q000502:physiology; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D010012:Osteogenesis / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D015032:Zinc / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite; D015032:Zinc