Isolation of uncultivable anaerobic thermophiles of the family Clostridiaceae requiring growth-supporting factors.
Novel groups of uncultivable anaerobic thermophiles were isolated from compost by enrichment cultivation in medium with a cell-free extract of Geobacillus toebii. The cell-free extract of G. toebii provided the medium with growth-supporting factors (GSF) needed to cultivate the previously uncultured microorganisms. Twenty-nine GSF-requiring candidates were successfully cultivated, and 16 isolated novel bacterial strains were classified into three different groups of uncultivable bacteria. The similarity among these 16 isolates and a phylogenetic analysis using 16S rRNA gene sequences revealed that these GSF-requiring strains represented novel groups within the family Clostridiaceae.
Kim
Joong-Jae
JJ
Department of Biological Sciences, Graduate School of Science, Osaka University, 1-1 Machikaneyama-cho, Toyonaka, Osaka 560-0043, Japan.
Kim
Hee Na
HN
Masui
Ryoji
R
Kuramitsu
Seili
S
Seo
Jin-Ho
JH
Kim
Kwang
K
Sung
Moon-Hee
MH
D000693:Anaerobiosis; D001406:Bacillaceae / Q000145:classification / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D018929:Cell Culture Techniques; D003470:Culture Media / Q000737:chemistry; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017385:Sequence Homology; D012988:Soil Microbiology
D003470:Culture Media; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S