Efficient and direct fermentation of starch to ethanol by sake yeast strains displaying fungal glucoamylases.
Aspergillus oryzae glucoamylases encoded by glaA and glaB, and Rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast Saccharomyces cerevisiae GRI-117-UK and laboratory yeast S. cerevisiae MT8-1. Among constructed transformants, GRI-117-UK/pUDGAA, displaying glaA glucoamylase, produced the most ethanol from liquefied starch, although MT8-1/pUDGAR, displaying R. oryzae glucoamylase, had the highest glucoamylase activity on its cell surface.
Kotaka
Atsushi
A
Research Institute, Gekkeikan Sake Co., Ltd., Fushimi-ku, Kyoto 612-8385, Japan. a_kotaka@gekkeikan.co.jp
Sahara
Hiroshi
H
Hata
Yoji
Y
Abe
Yasuhisa
Y
Kondo
Akihiko
A
Kato-Murai
Michiko
M
Kuroda
Kouichi
K
Ueda
Mitsuyoshi
M
D001236:Aspergillus oryzae / Q000201:enzymology; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D012233:Rhizopus / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D014920:Wine / Q000382:microbiology*
D000431:Ethanol; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase