[Culture of rabbit parotid acinar cells and evaluation of its excretion function in vitro].
OBJECTIVE
To obtain a large number of rabbit parotid acinar cells with excretion function in vitro.


METHODS
Rabbit parotid acinar cells were cultured in the reformed DMEM; The shape and structure of cultured cells were observed with phase contrast microscope; Pan cytokeratin(PCK), alpha-amylase and actin of the cultured parotid acinar cells were examined with immunocytochemical SP technique; the secretion of different generations was evaluated with positive expression status of alpha-amylase. SPSS10.0 software package for windows was used for Student's t test.


RESULTS
The acinar cells had the ability of proliferation, they could pass 4 generations and survive for 4 weeks. The specific immunocytochemical staining for PCK and alpha-amylase could be seen in the plasm and (or) membrane of parotid acinar cells, but not for actin. The positive expression of alpha-amylase in the primary generation, generation 1, 2 and 3 was 79.6%, 76%, 47.7% and 2.4%, respectively, which decreased gradually. There was a significant difference between the second generation and two earlier generations(P<0.05).


CONCLUSIONS
The primary and first generations of parotid acinar cells maintain their characteristics and proliferation in vitro,which is very useful for further experiment.
He
Wei
W
Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China. hewei1968@tom.com
Qiao
Yong-Ming
YM
Li
Long-Jiang
LJ
D061354:Acinar Cells; D000818:Animals; D002478:Cells, Cultured; D066298:In Vitro Techniques; D010306:Parotid Gland / Q000166:cytology*; D012333:RNA, Messenger; D011817:Rabbits; D000516:alpha-Amylases / Q000378:metabolism
D012333:RNA, Messenger; D000516:alpha-Amylases