Erythrocyte and platelet phospholipid fatty acids as markers of advanced non-small cell lung cancer: comparison with serum levels of sialic acid, TPS and Cyfra 21-1.
The phospholipid fatty acid profiles of erythrocytes and platelets from fifty patients with advanced non-small cell lung cancer were investigated using gas chromatography/mass spectrometry, followed by "ROC" curves analysis to gain novel biomarker information. Sialic acid and cytokeratins were also examined. Potentially useful fatty acid markers: Erythrocytes: phosphatidylcholine, 18:2n6 and 20:4n6; phosphatidylethanolamine, 22:4n6 and 22:6n3 + 24:1n9. Platelets: phosphatidylcholine, 22.0; phosphatidylethanolamine, 22:5n3 + 24:0. At the cut-off value to obtain maximum accuracy, the best biomarkers were found in platelets: phosphatidylserine + phosphatidylinositol (PS + PI), 21:0; sphyngomyelin: 20:1n9 and 22:1n9. All these fatty acids showed similar/higher diagnostic yields than the commonly used markers sialic acid or cytokeratins.
de Castro
Javier
J
Radiology Service, Santísima Trinidad Foundation Hospital, Salamanca, Spain.
Rodríguez
Marina C
MC
Martínez-Zorzano
Vicenta S
VS
Hernández-Hernández
Angel
A
Llanillo
Marcial
M
Sánchez-Yagüe
Jesús
J
D000230:Adenocarcinoma / Q000097:blood; D000368:Aged; D000951:Antigens, Neoplasm / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D001792:Blood Platelets / Q000737:chemistry*; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood; D004912:Erythrocytes / Q000737:chemistry*; D005227:Fatty Acids / Q000097:blood*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D053539:Keratin-19; D007633:Keratins / Q000097:blood*; D008175:Lung Neoplasms / Q000097:blood*; D008297:Male; D008875:Middle Aged; D019158:N-Acetylneuraminic Acid / Q000097:blood*; D009363:Neoplasm Proteins / Q000097:blood; D010455:Peptides / Q000097:blood*; D010743:Phospholipids / Q000097:blood*; D012372:ROC Curve; D012680:Sensitivity and Specificity
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D005227:Fatty Acids; D053539:Keratin-19; D009363:Neoplasm Proteins; D010455:Peptides; D010743:Phospholipids; C102012:antigen CYFRA21.1; C089984:tissue polypeptide specific antigen; D007633:Keratins; D019158:N-Acetylneuraminic Acid