Cloning and characterization of a gene encoding HMG-CoA reductase from Ganoderma lucidum and its functional identification in yeast.
A gene encoding 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR) was isolated from a triterpene-producing fungus, Ganoderma lucidum (Reishi or Lingzhi). This report provides the complete nucleotide sequence of the full-length cDNA encoding HMGR and its genomic DNA sequence. The cDNA of the HMGR (GenBank Accession no., EU263989) was found to contain an open reading frame (ORF) of 3,681 bp encoding a 1,226-amino-acid polypeptide, whereas the HMGR genomic DNA sequence (GenBank Accession no., EU263990) consisted of 4,262 bp and contained seven exons and six introns. The deduced amino acid sequence of G. lucidum HMGR showed significant homology to the known HMGRs from Ustilago maydis and Cryptococcus neoformans, and contained four conserved domains. Gene expression analysis showed that the expression level was relatively low in mycelia incubated for 10, 12, and 14 d, and reached the highest level in the primordia. Functional complementation of Gl-HMGR in a HMGR-deficient mutant yeast strain indicated that the cloned cDNA encoded a HMG-CoA reductase.
Shang
Chang-Hua
CH
College of Life Sciences, Nanjing Agricultural University Key Laboratory for Microbiological Engineering of the Agricultural Environment, Ministry of Agriculture.
Zhu
Fen
F
Li
Na
N
Ou-Yang
Xiang
X
Shi
Liang
L
Zhao
Ming-Wen
MW
Li
Yu-Xiang
YX
D000214:Acyl Coenzyme A / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D003001:Cloning, Molecular; D019295:Computational Biology; D018076:DNA, Complementary / Q000235:genetics; D004271:DNA, Fungal / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal / Q000235:genetics; D008969:Molecular Sequence Data; D020940:Reishi / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D014315:Triterpenes / Q000378:metabolism
D000214:Acyl Coenzyme A; D018076:DNA, Complementary; D004271:DNA, Fungal; D014315:Triterpenes; C008047:3-hydroxy-3-methylglutaryl-coenzyme A