title: Biogenesis of MalF and the MalFGK(2) maltose transport complex in Escherichia coli requires YidC.
abstract: The polytopic inner membrane protein MalF is a constituent of the MalFGK(2) maltose transport complex in Escherichia coli. We have studied the biogenesis of MalF using a combination of in vivo and in vitro approaches. MalF is targeted via the SRP pathway to the Sec/YidC insertion site. Despite close proximity of nascent MalF to YidC during insertion, YidC is not required for the insertion of MalF into the membrane. However, YidC is required for the stability of MalF and the formation of the MalFGK(2) maltose transport complex. Our data indicate that YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex.
authors.affiliation: Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
authors.forename: Samuel
authors.initials: S
authors.lastname: Wagner
authors.forename: Ovidiu Ioan
authors.initials: OI
authors.lastname: Pop
authors.forename: Ovidio
authors.initials: O
authors.lastname: Pop
authors.forename: Gert-Jan
authors.initials: GJ
authors.lastname: Haan
authors.forename: Louise
authors.initials: L
authors.lastname: Baars
authors.forename: Gregory
authors.initials: G
authors.lastname: Koningstein
authors.forename: Mirjam M
authors.initials: MM
authors.lastname: Klepsch
authors.forename: Pierre
authors.initials: P
authors.lastname: Genevaux
authors.forename: Joen
authors.initials: J
authors.lastname: Luirink
authors.forename: Jan-Willem
authors.initials: JW
authors.lastname: de Gier
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism / Q000502:physiology*; D001692:Biological Transport; D002462:Cell Membrane / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000378:metabolism / Q000502:physiology*; D015964:Gene Expression Regulation, Bacterial; D008320:Maltose / Q000378:metabolism*; D026901:Membrane Transport Proteins / Q000378:metabolism / Q000502:physiology*; D008954:Models, Biological; D009004:Monosaccharide Transport Proteins / Q000378:metabolism / Q000502:physiology*; D010957:Plasmids / Q000378:metabolism; D011485:Protein Binding; D011487:Protein Conformation; D017510:Protein Folding
chemical_list: D018528:ATP-Binding Cassette Transporters; D029968:Escherichia coli Proteins; C461639:MalF protein, E coli; C079123:MalG protein, E coli; C489398:MalK protein, E coli; D026901:Membrane Transport Proteins; D009004:Monosaccharide Transport Proteins; C412443:YIDC protein, E coli; D008320:Maltose