Polyethylenglycol-co-poly-D,L-lactide copolymer based microspheres: preparation, characterization and delivery of a model protein.
OBJECTIVE
To prepare and characterize polyethylenglycol-co-poly-D,L-lactide (PEG-D,L-PLA) multiblock copolymer microspheres containing ovalbumin. Microsphere batches made of Poly-D,L-lactide (PLA) homopolymers were prepared in order to evaluate how the presence of PEG segments into PEG-D,L-PLA copolymer could affect the behaviour of microspheres as carrier of protein drugs.


METHODS
The PEG-D,L-PLA and PLA microspheres, loaded with the model protein ovalbumin, were prepared using double emulsion solvent evaporation method. The effect of PEG segments in the microparticles matrix, on the morphology, size distribution, encapsulation efficiency and release behaviour was studied.


RESULTS
According to the results, PEG-D,L-PLA microspheres were more hydrophilic than PLA microparticles and with lower glass transition temperature. The surface of PEG-D,L-PLA microspheres was not as smooth as that of PLA microparticles, the mean diameter of PEG-D,L-PLA microparticles was bigger than that of PLA microspheres. Protein release from the microspheres was affected by the morphological structure of PEG-D,L-PLA microspheres and properties of PEG-D,L-PLA copolymer. This study suggests that PEG-D,L-PLA multiblock copolymer may be used as carrier in protein delivery systems for different purposes.
Dorati
R
R
Department of Pharmaceutical Chemistry, University of Pavia, Pavia, Italy.
Genta
I
I
Tomasi
C
C
Modena
T
T
Colonna
C
C
Pavanetto
F
F
Perugini
P
P
Conti
B
B
D002626:Chemistry, Pharmaceutical / Q000379:methods; D004337:Drug Carriers; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems; D015195:Drug Design; D006863:Hydrogen-Ion Concentration; D007773:Lactates / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D008970:Molecular Weight; D010047:Ovalbumin / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D013696:Temperature
D004337:Drug Carriers; D007773:Lactates; D011108:Polymers; C088513:poly(lactic acid-ethylene glycol); D011092:Polyethylene Glycols; D012965:Sodium Chloride; D010047:Ovalbumin