Disturbance of pro-oxidative/antioxidative balance in allogeneic peripheral blood stem cell transplantation.
High dose chemotherapy causes increased free radical formation and depletion of tissue antioxidants. Whether allogeneic hematopoietic stem cell transplantation (HSCT) has an effect on oxidative stress is uncertain. The aims of the study were to determine the effect of allogeneic HSCT on plasma concentrations of antioxidants and oxidative stress biomarkers, and to investigate their relationships with graft-versus-host disease (GVHD), conditioning regimens, and transplant-related mortality (TRM) in patients with hematological malignancies. Patients (n=25) undergoing allogeneic HSCT from HLA-matched sibling donors were enrolled in the study. Plasma oxidant and antioxidant status were measured at day -1 before transplantation and 30 days after HSCT. In both myeloablative (n=14) and non-myeloablative (n=11) transplant groups, the mean levels of plasma malondialdehyde (MDA) and nitric oxide (NO) increased after allogeneic HSCT (p <0.01), whereas superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities decreased compared with baseline values (p <0.01). No significant relationships were found between either the pretransplant or post-transplant mean levels of the oxidative stress parameters and the existence of graft-versus-host disease (GVHD), the type of conditioning regimen, or transplant related mortality (TRM). This study documents a significant disturbance of pro-oxidative/antioxidative balance in the plasma of patients undergoing allogeneic HSCT regardless of the intensity of the conditioning regimen.
Sari
Ismail
I
Department of Hematology, Pamukkale University Faculty of Medicine, TR-20070, Denizli, Turkey. hisari@pau.edu.tr <hisari@pau.edu.tr>
Cetin
Aysun
A
Kaynar
Leylagul
L
Saraymen
Recep
R
Hacioglu
Sibel Kabukcu
SK
Ozturk
Ahmet
A
Kocyigit
Ismail
I
Altuntas
Fevzi
F
Eser
Bulent
B
D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006086:Graft vs Host Disease / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D016877:Oxidants / Q000097:blood*; D018384:Oxidative Stress; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000097:blood; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous
D000975:Antioxidants; D015415:Biomarkers; D016877:Oxidants; D009569:Nitric Oxide; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase