Accumulation of phenylpropanoid derivatives in chitosan-induced cell suspension culture of Cocos nucifera.
Chitosan-induced elicitation responses of dark-incubated Cocos nucifera (coconut) endosperm cell suspension cultures led to the rapid formation of phenylpropanoid derivatives, which essentially mimics the defense-induced biochemical changes in coconut palm as observed under in vivo conditions. An enhanced accumulation of p-hydroxybenzoic acid as the major wall-bound phenolics was evident. This was followed by p-coumaric acid and ferulic acid. Along with enhanced peroxidases activities in elicited lines, the increase in activities of the early phenylpropanoid pathway enzymes such as, phenylalanine ammonia lyase (PAL), p-coumaroyl-CoA ligase (4CL) and p-hydroxybenzaldehyde dehydrogenase (HBD) in elicited cell cultures were also observed. Furthermore, supplementation of specific inhibitors of PAL, C4H and 4CL in elicited cell cultures led to suppressed accumulation of p-hydroxybenzoic acid, which opens up interesting questions regarding the probable route of the biosynthesis of this phenolic acid in C. nucifera.
Chakraborty
Moumita
M
Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, India.
Karun
Anitha
A
Mitra
Adinpunya
A
D000445:Aldehyde Oxidoreductases / Q000378:metabolism; D002473:Cell Wall / Q000187:drug effects / Q000378:metabolism; D002478:Cells, Cultured; D048271:Chitosan / Q000494:pharmacology*; D003059:Cocos / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D062385:Hydroxybenzoates / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D013997:Time Factors
D004791:Enzyme Inhibitors; D062385:Hydroxybenzoates; D010636:Phenols; D048271:Chitosan; D009195:Peroxidase; D000445:Aldehyde Oxidoreductases; C068611:4-hydroxybenzaldehyde dehydrogenase; D010650:Phenylalanine Ammonia-Lyase