Rapid identification of penicillin and macrolide resistance genes and simultaneous quantification of Streptococcus pneumoniae in purulent sputum samples by use of a novel real-time multiplex PCR assay.
We evaluated a real-time quantitative PCR combined with a multiplex PCR assay for the quantification of Streptococcus pneumoniae and the simultaneous detection of drug-resistant genes by gel-based PCR, using purulent sputum samples. This assay correctly quantified S. pneumoniae and identified their penicillin and erythromycin susceptibilities directly from samples within 3 h.
Fukushima
Kazuko Y
KY
Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki City 852-8501, Japan.
Yanagihara
Katsunori
K
Hirakata
Yoichi
Y
Sugahara
Kazuyuki
K
Morinaga
Yoshitomo
Y
Kohno
Shigeru
S
Kamihira
Shimeru
S
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D015169:Colony Count, Microbial / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D018942:Macrolides / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D010406:Penicillins / Q000494:pharmacology; D011008:Pneumococcal Infections / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D013183:Sputum / Q000382:microbiology*; D013296:Streptococcus pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D013492:Suppuration / Q000382:microbiology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D018942:Macrolides; D010406:Penicillins