title: Live single-cell molecular analysis by video-mass spectrometry.
abstract: The direct molecular analysis of a live single cell viewed under a video-microscope has been developed. The cell contents are sucked into a nano-electrospray tip, and hundreds of peaks of ionic compounds of low molecular weight are detected by nano-ESI Q-TOF mass spectrometry (MS). Cell-specific MS peaks in a single mouse-embryonic fibroblasts cell are extracted by a t-test, and one of the peaks is proceeded to MS/MS analysis for molecular identification. This method is direct and quick to identify the molecules of a cell with simultaneous observation by a video-microscope.
authors.affiliation: Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
authors.forename: Naohiro
authors.initials: N
authors.lastname: Tsuyama
authors.forename: Hajime
authors.initials: H
authors.lastname: Mizuno
authors.forename: Emi
authors.initials: E
authors.lastname: Tokunaga
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Masujima
mesh_terms: D000818:Animals; D002470:Cell Survival; D013058:Mass Spectrometry / Q000379:methods*; D051379:Mice; D015394:Molecular Structure; D011392:Proline / Q000737:chemistry; D041701:Swiss 3T3 Cells; D014743:Videotape Recording / Q000379:methods*
chemical_list: D011392:Proline