Observation of nanoparticle internalization on cellular membranes by using noninterferometric widefield optical profilometry.
We demonstrate the observation of gold-nanoparticle internalization in membranes of living cells by using noninterferometric widefield optical profilometry (NIWOP). The NIWOP technique can trace the height of an 80 nm gold particle on the membrane by calibrating the change of light intensity scattered from the particle along the optical axis. On the membrane, the depth resolution based on the scattering signal is similar to that based on the reflection signal, nearly 20 nm. Comparing the heights of the nanoparticle and the nearby cell membranes, we can identify the occurrence of particle internalization. Combining fluorescence microscopy with NIWOP, we also find actin aggregation around the site of the internalization process, which is an indication of endocytosis.
Wang
Chun-Chieh
CC
Graduate Institute of Physics, National Chung Cheng University, Chia-Yi 62102, Taiwan.
Lee
Chia-Wei
CW
Huang
Chia-Yun
CY
Lin
Jiunn-Yuan
JY
Wei
Pei-Kuen
PK
Lee
Chau-Hwang
CH
D016475:3T3 Cells; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism* / Q000648:ultrastructure; D006046:Gold; D008027:Light; D053768:Metal Nanoparticles / Q000648:ultrastructure; D051379:Mice; D055095:Optics and Photonics; D012542:Scattering, Radiation
D006046:Gold