title: Inhibition of tumor cell proliferation by Coleon C.
abstract: Coleon C (6,11,12,14,16-pentahydroxyabieta-5,8,11,13-tetraen-7-one), extracted from Coleus forskohlii Briq., was investigated for its anti-tumor activity on eight human tumor cell lines (95-D, A375, HeLa, A431, MKN45, BEL7402, LoVo and HL60) and two normal ones (293, L02) by MTT and colony-forming assay in vitro. The results indicated that A375 was the most sensitive of all the cell lines. Hoechst 33258 staining showed fragmentation and condensation of chromatin. DNA ladder assay indicated the fragments of DNA because of apoptosis. Flow cytometric analysis demonstrated hypodiploid cells existed in A375 after Coleon C treatment. In the acute toxicity studies of C57BL/6 mice, LD(50 )of Coleon C was 1496+/-150 mg/kg. In the model of Lewis lung carcinoma, the average tumor weight in groups injected with 80 mg/kg Coleon C decreased by 48.9+/-14.3% compared with that of the control. These results indicate that Coleon C could effectively inhibit tumor cell proliferation and growth by inducing apoptosis with low toxicity. To our knowledge, this is the first report on the anti-tumor activity of Coleon C both in vitro and in vivo.
authors.affiliation: State Key Laboratory of Bioreactor Engineering and School of Pharmacy, East China University of Science and Technology, Shanghai, PR China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Xing
authors.forename: Hezhen
authors.initials: H
authors.lastname: Wu
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Wang
authors.forename: Yongping
authors.initials: Y
authors.lastname: Huang
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.forename: Changlong
authors.initials: C
authors.lastname: Li
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Yang
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Liu
authors.forename: Jianwen
authors.initials: J
authors.lastname: Liu
mesh_terms: D045784:Abietanes / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D031330:Coleus; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D007928:Lethal Dose 50; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: C530132:6,11,12,14,16-pentahydroxyabieta-5,8,11,13-tetraen-7-one; D045784:Abietanes; D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts