Retrovirus vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation.
Owing to its external position in the embryo, the chick eye has been used as a readily accessible model for studying the molecular mechanisms behind the patterning of the central nervous system. Although methods of genetic analysis have not been established as in the mouse, the chick is convenient for analyzing the functions of genes by in ovo electroporation of retroviral vectors. In this review, we describe the retroviral vector-mediated transfer of genes into the chick optic vesicle by in ovo electroporation. A rapid, efficient, and sustained expression of transgenes is achieved by this approach.
Sakuta
Hiraki
H
Division of Molecular Neurobiology, National Institute for Basic Biology, and School of Life Science, The Graduate University for Advanced Studies, Okazaki 444-8787, Japan.
Suzuki
Ryoko
R
Noda
Masaharu
M
D000818:Animals; D002642:Chick Embryo; D004247:DNA / Q000378:metabolism; D004566:Electrodes; D018274:Electroporation / Q000379:methods*; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000378:metabolism; D051379:Mice; D008957:Models, Genetic; D010957:Plasmids / Q000378:metabolism; D012160:Retina / Q000196:embryology*; D012190:Retroviridae / Q000235:genetics*; D014162:Transfection; D019076:Transgenes
D049452:Green Fluorescent Proteins; D004247:DNA