[Expression and implication of osteopontin in renal cortex of diabetic rats].
OBJECTIVE
To investigate the expression and implication of osteopontin (OPN) in renal cortex of diabetic rats.


METHODS
Experimental diabetes was reproduced in uninephrectomized rats by giving streptozotocin (STZ). The animals were divided into two groups: control group and diabetic group (each n=24). Immunohistochemistry and Western blotting were used to detect the protein expression of OPN, CD68, and proliferating cell nuclear antigen (PCNA). At the same time the mRNA expression of OPN was detected by in situ hybridization.


RESULTS
Compared with control group, blood glucose, blood urea nitrogen (BUN), 24-hour urine protein quantum and quantification of OPN protein significantly increased while creatinine clearance rate (CCr) was significantly decreased in diabetic rat group 1, 2, 4 and 8 weeks after STZ injection. The protein of PCNA were also higher than that of control group 1 and 2 weeks after STZ injection (all P<0.05), and the increase in CD68 was observed at week 4 and week 8.


CONCLUSIONS
Overexpression of OPN may be responsible for the proliferation of tubular cells at early stage of diabetic nephropathy and for the accumulation of macrophage at later stage.
Cao
Yan-Ping
YP
Department of Pathology, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
Gao
Feng
F
Hao
Jun
J
Ren
Yun-Zhuo
YZ
Duan
Hui-Jun
HJ
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000097382:CD68 Molecule
D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D053495:Osteopontin; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; C539355:CD68 protein, rat; D000097382:CD68 Molecule