title: Melanoblasts' proper location and timed differentiation depend on Notch/RBP-J signaling in postnatal hair follicles.
abstract: The Notch/RBP-J pathway is involved in a variety of developmental processes and in tissue homeostasis. In the melanocyte lineage, it has been shown that Notch signaling acts through Hes1 to maintain the melanocyte stem cell population in the hair follicle. This study was designed to determine whether Notch signaling is implicated in other steps of melanocyte-lineage postnatal development. For this purpose, we developed mice in which the RBP-J gene was conditionally ablated in the melanocyte lineage and used the Dct-lacZ reporter transgene to track melanocytes and their precursors in individual hair follicles. We determine that Notch/RBP-J-deficient melanoblasts are in reduced number within the hair follicle and gather within its lower permanent part. Moreover, our results show that Notch signaling is necessary to prevent differentiation of melanocyte stem cells and of melanoblasts before they reach the hair bulb. Finally, our data show that Notch signaling is involved in proper location of melanoblasts in the outer root sheath and of melanocytes in the hair matrix. These findings reveal previously unrecognized roles for Notch signaling in the melanocyte lineage.
authors.affiliation: INRA, UMR955 Génétique Moléculaire et Cellulaire; Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort, France; Institut Pasteur, Unité de Génétique Fonctionnelle de la Souris; CNRS, URA 2578, Département de Biologie du Développement; USC INRA, Paris, France. Electronic address: ghouzelstein@vet-alfort.fr.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Aubin-Houzelstein
authors.affiliation: INRA, UMR955 Génétique Moléculaire et Cellulaire; Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort, France; Institut Pasteur, Unité de Génétique Fonctionnelle de la Souris; CNRS, URA 2578, Département de Biologie du Développement; USC INRA, Paris, France.
authors.forename: Johanna
authors.initials: J
authors.lastname: Djian-Zaouche
authors.affiliation: INRA, UMR955 Génétique Moléculaire et Cellulaire; Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Bernex
authors.affiliation: INRA, UMR955 Génétique Moléculaire et Cellulaire; Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Gadin
authors.affiliation: CNRS, UMR146 Génétique du Développement des Mélanocytes; Institut Curie, Bât. 110, Orsay, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Delmas
authors.affiliation: CNRS, UMR146 Génétique du Développement des Mélanocytes; Institut Curie, Bât. 110, Orsay, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Larue
authors.affiliation: INRA, UMR955 Génétique Moléculaire et Cellulaire; Ecole Nationale Vétérinaire d'Alfort, Maisons-Alfort, France; Institut Pasteur, Unité de Génétique Fonctionnelle de la Souris; CNRS, URA 2578, Département de Biologie du Développement; USC INRA, Paris, France. Electronic address: panthier@pasteur.fr.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Panthier
mesh_terms: D000818:Animals; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology*; D019070:Cell Lineage / Q000502:physiology; D002465:Cell Movement / Q000502:physiology; D018859:Hair Follicle / Q000166:cytology / Q000378:metabolism*; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism; D007763:Lac Operon / Q000235:genetics; D008544:Melanocytes / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D019076:Transgenes / Q000235:genetics
chemical_list: D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; C497571:Rbpj protein, mouse; D051880:Receptors, Notch; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase