title: Prostanoids in bronchoalveolar lavage fluid do not predict outcome in congenital diaphragmatic hernia patients.
abstract: Vasoactive prostanoids may be involved in persistent pulmonary hypertension (PPH) in infants with a congenital diaphragmatic hernia (CDH). We hypothesized that increased levels of prostanoids in bronchoalveolar lavage (BAL) fluid would predict clinical outcome. We measured the concentrations of 6-keto-prostaglandin F(1alpha) (6-keto-PGF(1alpha)), thromboxane B(2) (TxB(2)), protein, albumin, total cell count, and elastase-alpha1-proteinase-inhibitor complex in BAL fluid of 18 CDH patients and of 13 control subjects without PPH. We found different concentrations of prostanoids in BAL fluid of CDH patients with PPH: infants with a poor prognosis had either high levels of both 6-keto-PGF(1alpha) and TxB(2) compared to controls, or high levels of 6-keto-PGF(1alpha) only. TxB(2) levels showed a large variability in all CDH patients irrespective of outcome. We conclude that prostanoid levels in BAL fluid do not predict clinical outcome in CDH patients.
authors.affiliation: Department of Paediatric Surgery Erasmus University Rotterdam and University Hospital/Sophia Children's Hospital Rotterdam The Netherlands.
authors.forename: H
authors.initials: H
authors.lastname: Ijsselstijn
authors.forename: F J
authors.initials: FJ
authors.lastname: Zijlstra
authors.forename: J C
authors.initials: JC
authors.lastname: De Jongste
authors.forename: D
authors.initials: D
authors.lastname: Tibboel