title: The hydrophobic segment of Arabidopsis thaliana cluster I diacylglycerol kinases is sufficient to target the proteins to cell membranes.
abstract: Diacylglycerol kinases (DGKs) catalyze the phosphorylation of diacylglycerol into phosphatidic acid. To fulfill their role in many signalling processes, DGKs must be located at, or in, membranes. Most mammalian DGKs are cytosolic and are recruited to membranes upon stimulation, except for epsilon type DGKs that are permanently membrane-associated through a hydrophobic segment. Nothing is known about the mechanism(s) involved in the membrane localization of plant DGKs. By fusion to fluorescent proteins, we show that two DGKs from cluster I in Arabidopsis thaliana possess amino-terminal hydrophobic segments that are sufficient to address them to endoplasmic reticulum membranes.
authors.affiliation: UPMC Univ Paris 06, UMR 7180, Physiologie Cellulaire et Moléculaire des Plantes, F-94200 Ivry-sur-Seine, France.
authors.forename: Marie-Noëlle
authors.initials: MN
authors.lastname: Vaultier
authors.forename: Catherine
authors.initials: C
authors.lastname: Cantrel
authors.forename: Françoise
authors.initials: F
authors.lastname: Guerbette
authors.forename: Yohann
authors.initials: Y
authors.lastname: Boutté
authors.forename: Chantal
authors.initials: C
authors.lastname: Vergnolle
authors.forename: Dominique
authors.initials: D
authors.lastname: Ciçek
authors.forename: Susanne
authors.initials: S
authors.lastname: Bolte
authors.forename: Alain
authors.initials: A
authors.lastname: Zachowski
authors.forename: Eric
authors.initials: E
authors.lastname: Ruelland
mesh_terms: D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology*; D017124:Conserved Sequence; D019852:Diacylglycerol Kinase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D004721:Endoplasmic Reticulum / Q000201:enzymology*; D057927:Hydrophobic and Hydrophilic Interactions; D007425:Intracellular Membranes / Q000201:enzymology*; D008969:Molecular Sequence Data; D017434:Protein Structure, Tertiary
chemical_list: D019852:Diacylglycerol Kinase