Toponomics and neurotoponomics: a new way to medical systems biology.
The fluorescence robot imaging technology multi-epitope-ligand-cartography/toponome imaging system has revolutionized the field of proteomics/functional genomics, because it enables the investigator to locate and decipher functional protein networks, the toponome, consisting of hundreds of different proteins in a single cell or tissue section. The technology has been proven to solve key problems in biology and therapy research. It has uncovered a new cellular transdifferentiation mechanism of vascular cells giving rise to myogenic cells in situ and in vivo; a finding that has led to efficient cell therapy models of muscle disorders, and discovered a new target protein in sporadic amyotrophic lateral sclerosis by hierarchical protein network analysis, a finding that has been confirmed by a mouse knockout model. A lead target protein in tumor cells that controls cell polarization as a mechanism that is fundamental for migration and metastasis formation has also been uncovered, and new functional territories in the CNS defined by high-dimensional synaptic protein clusters have been unveiled. The technology can be effectively interlocked with genomics and proteomics to optimize time-to-market and the overall attrition rate of new drugs. This review outlines major proofs of principle with an emphasis on neurotoponomics.
Schubert
Walter
W
Otto-von-Guericke-University Magdeburg, Molecular Pattern Recognition Research Group, Institute of Medical Neurobiology, Medical Faculty, 39120 Magdeburg, Germany. walter.schubert@med.ovgu.de
Bode
Marcus
M
Hillert
Reyk
R
Krusche
Andreas
A
Friedenberger
Manuela
M
D000818:Animals; D006801:Humans; D009419:Nerve Tissue Proteins / Q000032:analysis*; D015388:Organelles / Q000737:chemistry*; D040901:Proteomics / Q000379:methods*; D049490:Systems Biology
D009419:Nerve Tissue Proteins