title: Mononuclear phagocytes in the blood of turtles characterized by ultrastructural and cytochemical analyses and by phagocytic activity.
abstract: Ultrastructural and cytochemical characteristics of mononuclear phagocyte cells in turtles are not well described in the literature, especially in Phrynops hilarii. Thus, the aim of this study was to evaluate these characteristics in the mononuclear phagocyte cells and their phagocytic activity "in vitro" using the turtle P. hilarii as an experimental animal model. The six turtles used in the study were observed in two seasons, spring and summer. Results showed that mononuclear phagocytes incubated only in diluted solution or with colloidal charcoal have cytoplasm phagolysosomes. The cells incubated with colloidal charcoal and further exposed to the cytochemical reaction for acid beta-glycerophosphatase, showed cytoplasm phagolysosomes filled by charcoal particles being digested and some positively stained lysosomes. Acid beta-glycerophosphatase positive reaction was present in lysosomes and inside the phagolysosomes, while acid cytidine 5-monophosphatase staining occurred in lysosome surroundings. A positive reaction for trimetaphosphatase was also found inside phagolysosomes. In conclusion, the presence of lysosomal enzymes like trimetaphosphatase and cytidine-5'-sodium monophosphate, in the circulating blood of P. hilarii indicate that mononuclear phagocytes participate in the phagocytic process by gathering many phagocytic cells and forming multinucleated giant cells, which probably have a role in the blood "clearance" process.
authors.affiliation: Biosciences Institute, Molecular and Cellular Biology (UNESP), Rio Claro, São Paulo, Brazil.
authors.forename: Dimitrius Leonardo
authors.initials: DL
authors.lastname: Pitol
authors.forename: João Paulo Mardegan
authors.initials: JP
authors.lastname: Issa
authors.forename: Flávio Henrique
authors.initials: FH
authors.lastname: Caetano
authors.forename: Laurelúcia Orive
authors.initials: LO
authors.lastname: Lunardi
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000032:analysis; D000818:Animals; D003568:Cytidine Monophosphate / Q000032:analysis; D006651:Histocytochemistry; D008247:Lysosomes / Q000201:enzymology; D010586:Phagocytes / Q000201:enzymology / Q000502:physiology / Q000648:ultrastructure*; D010587:Phagocytosis; D010744:Phosphoric Monoester Hydrolases / Q000032:analysis; D014426:Turtles / Q000097:blood*
chemical_list: C020674:glycerol-2-phosphatase; D010744:Phosphoric Monoester Hydrolases; D017766:Acid Anhydride Hydrolases; C022768:trimetaphosphatase; D003568:Cytidine Monophosphate