title: Bacillus anthracis spores influence ATP synthase activity in murine macrophages.
abstract: Anthrax is an infectious disease caused by toxigenic strains of the Gram-positive bacterium Bacillus anthracis. To identify the mitochondrial proteins that are expressed differently in murine macrophages infected with spores of B. anthracis Sterne, proteomic and MALDI-TOF/MS analyses of uninfected and infected macrophages were conducted. As a result, 13 mitochondrial proteins with different expression patterns were discovered in the infected murine macrophages, and some were identified as ATP5b, NIAP-5, ras-related GTP binding protein B isoform CRAa, along with several unnamed proteins. Among these proteins, ATP5b is related to energy production and cytoskeletal rearrangement, whereas NIAP-5 causes apoptosis of host cells due to binding with caspase-9. Therefore, this paper focused on ATP5b, which was found to be downregulated following infection. The downregulated ATP5b also reduced ATP production in the murine macrophages infected with B. anthracis spores. Consequently, this study represents the first mitochondrial proteome analysis of infected macrophages.
authors.affiliation: Division of Molecular and Life Sciences, Hanyang University, Ansan 426-791, Korea.
authors.forename: Gwi-Moon
authors.initials: GM
authors.lastname: Seo
authors.forename: Kyoung Hwa
authors.initials: KH
authors.lastname: Jung
authors.forename: Seong-Joo
authors.initials: SJ
authors.lastname: Kim
authors.forename: Ji-Cheon
authors.initials: JC
authors.lastname: Kim
authors.forename: Jang Won
authors.initials: JW
authors.lastname: Yoon
authors.forename: Kwang-Keun
authors.initials: KK
authors.lastname: Oh
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Lee
authors.forename: Young Gyu
authors.initials: YG
authors.lastname: Chai
mesh_terms: D000818:Animals; D000881:Anthrax / Q000201:enzymology / Q000378:metabolism / Q000382:microbiology*; D001408:Bacillus anthracis / Q000502:physiology*; D045744:Cell Line, Tumor; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008264:Macrophages / Q000737:chemistry / Q000201:enzymology* / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D024101:Mitochondrial Proteins / Q000737:chemistry / Q000378:metabolism; D025261:Mitochondrial Proton-Translocating ATPases / Q000737:chemistry / Q000378:metabolism*; D010449:Peptide Mapping; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013171:Spores, Bacterial / Q000502:physiology
chemical_list: C506526:ATP5F1B protein, human; D024101:Mitochondrial Proteins; D025261:Mitochondrial Proton-Translocating ATPases