Transient expression of phospholipase D1 during heart development in rats.
The expression of phospholipase D (PLD) isozymes was examined in the hearts of rats at different stages of development. Immunoprecipitation and Western blot analysis revealed weak PLD1 expression in the hearts of day 17 embryos. The level of PLD1 protein increased transiently 0 and 3 days postpartum, and declined gradually beginning 7 days after birth. Immunohistochemistry revealed weak PLD1 immunostaining in some cells at embryonic day 17. In contrast, some vascular endothelial cells and cardiomyocytes were immunostained typically at days 0, 3, and 7 after birth. After postnatal day 21, weak PLD1 expression was immunodetected in some vascular endothelial cells and cardiomyocytes. This suggests that the PLD1 protein in the heart is strongly associated with the early postnatal development of the heart in rats.
Moon
Changjong
C
Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
Kim
Heechul
H
Kim
Seungjoon
S
Lee
Yongduk
Y
Shin
Min Kyoung
MK
Min
Do Sik
do S
Shin
Taekyun
T
D000818:Animals; D005786:Gene Expression Regulation; D006321:Heart / Q000196:embryology*; D009206:Myocardium; D010739:Phospholipase D / Q000378:metabolism*; D051381:Rats
D010739:Phospholipase D; C106801:phospholipase D1