CD133+ cells from human peripheral blood promote corticospinal axon regeneration.
To assess the effect of endothelial progenitor cells on the axon growth of cortex neurons, we transplanted CD133 cells derived from human peripheral blood to an organotypic coculture system consisting of spinal cord and cortex from neonatal rats. The axon growth from cortex to spinal cord was significantly promoted in cultures after CD133 cells transplantation compared with that of the control cultures. In addition, real-time reverse transcription-PCR showed a significant upregulation of vascular endothelial growth factor mRNA in the spinal cord of the cultures containing CD133 cells. In contrast, the transplanted cells did not differentiate into endothelial cells. These data suggest that CD133 cells may promote axonal regeneration by upregulating vascular endothelial growth factor mRNA in spinal cord tissues.
Yamamoto
Risako
R
Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan. riyamamoto@hiroshima-u.ac.jp
Ishikawa
Masakazu
M
Tanaka
Nobuhiro
N
Kamei
Naosuke
N
Nakanishi
Kazuyoshi
K
Sasaki
Hirofumi
H
Nakamae
Toshio
T
Mochizuki
Yu
Y
Asahara
Takayuki
T
Ochi
Mitsuo
M
D000071916:AC133 Antigen; D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000378:metabolism*; D001369:Axons / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology*; D018920:Coculture Techniques; D006023:Glycoproteins / Q000378:metabolism*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D009416:Nerve Regeneration / Q000502:physiology*; D009924:Organ Culture Techniques; D010455:Peptides / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology*; D015854:Up-Regulation / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606025:Prom1 protein, rat; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat