A cross-species quantitative proteomic study of salt adaptation in a halotolerant environmental isolate using 15N metabolic labelling.
We examine differential protein expression in Euhalothece sp. BAA001, an extremely halotolerant and unsequenced cyanobacterium, under adaptation to low (0% w/v), medium (3% w/v), high (6% w/v) and very high (9% w/v) salt concentrations using cross-species protein identification tools. We combine stable isotope labelling with 1-D SDS-PAGE, and MASCOT protein identification software with MS-driven BLAST searches, to produce an accurate method for protein identification and quantitation. The use of metabolic labelling to improve the confidence in identification of proteins in cross-species proteomics is demonstrated. Three hundred and eighty-three unique proteins were identified, and 72 were deemed to be differentially expressed (average CV for quantitations was 0.10 +/- 0.08), belonging to 24 functional groups. Responses to low salt as well as high salt are discussed in terms of adaptation and evidence shows that Euhalothece cells display 'stress' responses in nonsaline conditions as well as higher salt environments.
Pandhal
Jagroop
J
Biological and Environmental Systems Group, Department of Chemical and Process Engineering, University of Sheffield, Sheffield, UK.
Snijders
Ambrosius P L
AP
Wright
Phillip C
PC
Biggs
Catherine A
CA
D002244:Carbon / Q000737:chemistry; D048429:Cell Size; D000458:Cyanobacteria / Q000378:metabolism*; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D004777:Environment; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D013058:Mass Spectrometry; D009587:Nitrogen Isotopes / Q000737:chemistry; D016133:Polymerase Chain Reaction; D040901:Proteomics / Q000379:methods*; D012492:Salts / Q000737:chemistry / Q000494:pharmacology; D012984:Software; D013045:Species Specificity
D009587:Nitrogen Isotopes; D012492:Salts; D002244:Carbon