title: Enhanced production of laccase from Coriolus versicolor NCIM 996 by nutrient optimization using response surface methodology.
abstract: Plackett and Burman design criterion and central composite design were applied successfully for enhanced production of laccase by Coriolus versicolor NCIM 996 for the first time. Plackett and Burman design criterion was applied to screen the significance of ten nutrients on laccase production by C. versicolor NCIM 996. Out of the ten nutrients tested, starch, yeast extract, MnSO(4), MgSO(4) x 7H(2)O, and phenol were found to have significant effect on laccase production. A central composite design was applied to determine the optimum concentrations of the significant variables obtained from Plackett-Burman design. The optimized medium composition for production of laccase was (g/l): starch, 30.0; yeast extract, 4.53; MnSO(4), 0.002; MgSO(4) x 7H(2)O, 0.755; and phenol, 0.026, and the optimum laccase production was 6,590.26 (U/l), which was 7.6 times greater than the control.
authors.affiliation: Department of Biotechnology, Kalasalingam University, Krishnankoil, Tamil Nadu, India. asanthiagu@rediffmail.com
authors.forename: Santhiagu
authors.initials: S
authors.lastname: Arockiasamy
authors.forename: Indira Packialakshmi Gurusamy
authors.initials: IP
authors.lastname: Krishnan
authors.forename: Nimalanandan
authors.initials: N
authors.lastname: Anandakrishnan
authors.forename: Sabitha
authors.initials: S
authors.lastname: Seenivasan
authors.forename: Agalya
authors.initials: A
authors.lastname: Sambath
authors.forename: Janani Priya
authors.initials: JP
authors.lastname: Venkatasubramani
mesh_terms: D003470:Culture Media; D007218:Industrial Microbiology / Q000379:methods; D042845:Laccase / Q000096:biosynthesis*; D055454:Trametes / Q000201:enzymology*
chemical_list: D003470:Culture Media; D042845:Laccase