title: Use of laser microdissection for phylogenetic characterization of polyphosphate-accumulating bacteria.
abstract: Our novel approach for taxonomic identification of uncultured bacteria harboring specific physiological features in complex environmental samples combines cell collection by laser microdissection and subsequent DNA analysis. The newly developed approach was successfully tested for collection and phylogenetic characterization of polyphosphate-accumulating bacteria in activated sludge and lake sediment.
authors.affiliation: Leibniz Institute of Freshwater Ecology and Inland Fisheries, Alte Fischerhütte 2, D-16775 Stechlin-Neuglobsow, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Gloess
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Grossart
authors.forename: Martin
authors.initials: M
authors.lastname: Allgaier
authors.forename: Stefan
authors.initials: S
authors.lastname: Ratering
authors.forename: Michael
authors.initials: M
authors.lastname: Hupfer
mesh_terms: D001419:Bacteria / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D004210:Dissection; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D007834:Lasers; D008853:Microscopy; D008969:Molecular Sequence Data; D010802:Phylogeny; D011122:Polyphosphates / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*
chemical_list: D011122:Polyphosphates; D012336:RNA, Ribosomal, 16S; D012722:Sewage