Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic.
The results of seven serologic tests for diagnosis of human brucellosis were evaluated. The titrated Rose Bengal test, microagglutination test, microtiter-adapted Coombs test, and immunocapture-agglutination test (Brucellacapt) were positive for all sera from patients with acute brucellosis. The immunoglobulin G (IgG), IgM, and IgA commercial enzyme immunoassays (ELISAs) failed to show specific antibodies in 3 patients, 10 patients, and 1 patient, respectively. The sensitivity of ELISA is not higher than that of conventional tests.
Gómez
M Concepción
MC
Microbiology Laboratory Hospital Severo Ochoa (Leganés), Madrid, Spain. mcongocri@hotmail.com
Nieto
José A
JA
Rosa
Carmen
C
Geijo
Paloma
P
Escribano
M Angeles
MA
Muñoz
Amparo
A
López
Clara
C
D000372:Agglutination Tests; D002002:Brucella / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D003298:Coombs Test; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012680:Sensitivity and Specificity; D012698:Serologic Tests