Production of cyclodextrins in ultrafiltration membrane reactor containing cyclodextrin glycosyltransferase from Bacillus macerans.
An enzyme reactor installed with ultrafiltration membrane was developed to produce alpha-, beta-, and gamma-cyclodextrins (CDs) from soluble starch by Bacillus macerans cyclodextrin glycosyltransferase (CGTase) tagged with 10 lysines at its C-terminus (CGTK10ase). Ultrafiltration membrane YM10 with 10,000 of molecular cutoff was chosen for membrane modification and CD production. A repeated-batch type of the enzyme reaction with free CGTK10ase resulted in a alpha-CD yield of 24.0 (+/-1.5)% and a productivity of 4.68 (+/-0.88) g/l-h, which were 7 times higher that those for CGTK10ase immobilized on modified YM10 membrane. Addition of 1- nonanol increased CD yields by 30% relative to the control, which might be due to prevention of the reversible hydrolysis of CDs.
Son
Young-Jin
YJ
Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul 151-742, Korea.
Rha
Chan-Su
CS
Park
Yong-Cheol
YC
Shin
So-Yeon
SY
Lee
Yoon-Seung
YS
Seo
Jin-Ho
JH
D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000032:analysis / Q000378:metabolism; D019149:Bioreactors; D003505:Cyclodextrins / Q000032:analysis* / Q000378:metabolism; D004800:Enzymes, Immobilized / Q000032:analysis* / Q000378:metabolism; D000431:Ethanol / Q000032:analysis / Q000378:metabolism; D005964:Glucosyltransferases / Q000032:analysis* / Q000378:metabolism; D006868:Hydrolysis; D008851:Micropore Filters; D013213:Starch / Q000032:analysis / Q000378:metabolism; D014462:Ultrafiltration / Q000379:methods*
D001426:Bacterial Proteins; D003505:Cyclodextrins; D004800:Enzymes, Immobilized; D000431:Ethanol; D013213:Starch; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase