title: Bioassay-directed purification of an acidic phospholipase A(2) from Agkistrodon halys pallas venom.
abstract: Relying on ex vivo and in vitro platelet anti-aggregation assays, a tail bleeding time assay, and an anti-thrombotic assay, we have purified the fraction of venom from Agkistrodon halys pallas which, in all of these assays, is the most active. There were two major steps in the purification: gel filtration chromatography on Sephadex G-100, and ion exchange chromatography on DEAE Sephadex A-50. Sequencing of the most active fraction by mass spectrometry revealed that it is a known acidic phospholipase A(2). Prior expectations by others about the in vivo anti-thrombotic activity of this enzyme are confirmed.
authors.affiliation: College of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, PR China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Wang
authors.forename: Guohui
authors.initials: G
authors.lastname: Cui
authors.forename: Ming
authors.initials: M
authors.lastname: Zhao
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.forename: Roger W
authors.initials: RW
authors.lastname: Giese
authors.forename: Shiqi
authors.initials: S
authors.lastname: Peng
mesh_terms: D017836:Agkistrodon; D000818:Animals; D000925:Anticoagulants / Q000302:isolation & purification* / Q000494:pharmacology; D001681:Biological Assay; D001760:Bleeding Time; D005591:Chemical Fractionation; D002845:Chromatography; D003435:Crotalid Venoms / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010449:Peptide Mapping; D054467:Phospholipases A2 / Q000302:isolation & purification* / Q000494:pharmacology; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D013927:Thrombosis / Q000188:drug therapy
chemical_list: D000925:Anticoagulants; D003435:Crotalid Venoms; D054467:Phospholipases A2