title: General concept of high-performance amperometric detector for microfluidic (bio)analytical chips.
abstract: In this work, we established theoretically that amperometric detector arrays consisting of a series of parallel band microelectrodes placed on the wall of a microchannel may offer excellent analytical detection performances when implemented onto microfluidic (bio)analytical devices after the separative stages. In combination with the concentration imprinting strategies reported in a previous work, these exceptional performances may be extended to nonelectroactive or poorly diffusing analytes. Using an array of electrodes instead of a large single band allows the whole core of the channel to be probed though keeping an excellent time resolution. Thus, analytes with close retention times may be characterized individually with a resolution which eventually outpaces that of spectroscopic detections. Such important advantages may be obtained only through a complete understanding of the complex coupling between diffusional and convective transport of molecules in microfluidic solutions near an electrochemical detector. As a consequence, the conditions underlying the theoretical data presented in this work have been selected after optimizing procedures rooted on previous theoretical analyses. They will be fully disclosed in a series of further works that will also establish the experimental performances of such amperometric detectors and validate the present concept.
authors.affiliation: UMR 8640 Pasteur, Département de Chimie, Ecole Normale Supérieure, CNRS and Université Pierre et Marie Curie 24, rue Lhomond, 75231 Paris Cedex 05, France. christian.amatore@ens.fr
authors.forename: Christian
authors.initials: C
authors.lastname: Amatore
authors.forename: Nicolas
authors.initials: N
authors.lastname: Da Mota
authors.forename: Catherine
authors.initials: C
authors.lastname: Sella
authors.forename: Laurent
authors.initials: L
authors.lastname: Thouin
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods