title: Cytocompatibility and hemocompatibility of a novel chitosan-alginate gel system.
abstract: Two chitosan-alginate gel systems in the form of membranes were produced and evaluated. The first membrane was produced by a novel gel system formed after blending N-(methylsulfonic acid) chitosan with ammonium alginate (CAG1) and the second was an N-(methylsulfonic acid) chitosan-sodium alginate blend cross-linked with glutaraldehyde and calcium chloride (CAG2). The cytocompatibility and hemocompatibility of the gels were examined by assessing the cell viability of 3T3 Swiss mouse fibroblasts, whole blood hemolysis, and platelet activation. Cell viability was not significantly different by exposure to these gels compared to the controls. Both gel types had minimal effect on hemolysis of whole heparinized rabbit blood after 1-h exposure. Further platelet activation by the surfaces was also minimal. These results indicate that these novel gels merit further investigation for blood contact applications.
authors.affiliation: Bioengineering Group, School of Mechanical, Materials and Manufacturing Engineering, University of Nottingham, Nottingham, NG7 2RD, United Kingdom. m.notara@ucl.ac.uk
authors.forename: M
authors.initials: M
authors.lastname: Notara
authors.forename: C A
authors.initials: CA
authors.lastname: Scotchford
authors.forename: D M
authors.initials: DM
authors.lastname: Grant
authors.forename: N
authors.initials: N
authors.lastname: Weston
authors.forename: G A F
authors.initials: GA
authors.lastname: Roberts
mesh_terms: D016475:3T3 Cells; D000327:Adsorption / Q000187:drug effects; D000464:Alginates / Q000494:pharmacology*; D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D005353:Fibronectins / Q000378:metabolism; D005782:Gels; D020723:Glucuronic Acid / Q000494:pharmacology; D006454:Hemoglobins; D006461:Hemolysis / Q000187:drug effects; D006603:Hexuronic Acids / Q000494:pharmacology; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D015539:Platelet Activation / Q000187:drug effects; D010973:Platelet Adhesiveness / Q000187:drug effects; D011817:Rabbits; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013499:Surface Properties / Q000187:drug effects
chemical_list: D000464:Alginates; D001672:Biocompatible Materials; D005353:Fibronectins; D005782:Gels; D006454:Hemoglobins; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D012710:Serum Albumin, Bovine; D020723:Glucuronic Acid; D048271:Chitosan