title: Organic extracts of Larrea divaricata Cav. induced apoptosis on tumoral MCF7 cells with an higher cytotoxicity than nordihydroguaiaretic acid or paclitaxel.
abstract: The methanolic and methane dichloride extracts of aerial parts of Larrea divaricata Cay. (Jarilla) plants exhibited a pronounced cytotoxic effect by arresting cell viability at a level of 35% against the MCF-7 cell line, a human breast adenocarcinoma, while only a weak cytotoxic effect was observed for the aqueous extract. Apoptosis was detected by flow cytometry, using annexin V and by cytological detection with fluorescein diacetate / propidium iodide staining. The annexin V, as well as the uptake of fluorescein diacetate/propidium iodide staining, revealed that methanolic and methane dichloride extracts of L. divaricata treatment of cells resulted in a rapid plasma membrane perturbation and triggered cellular death (>70%). Methanolic extracts were submitted to further treatment by bioassay-guided purification, involving fractionation and chromatography. 0.1% (w/w) nordihydroguaiaretic acid (NDGA) was found, which displayed weak citotoxic activity.
authors.affiliation: Instituto de Biología Celular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba. gbongiovanni@ceprocor.uncor.edu
authors.forename: Guillermina
authors.initials: G
authors.lastname: Bongiovanni
authors.forename: Juan
authors.initials: J
authors.lastname: Cantero
authors.forename: Aldo
authors.initials: A
authors.lastname: Eynard
authors.forename: Marta
authors.initials: M
authors.lastname: Goleniowski
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000473:pathology; D045744:Cell Line, Tumor / Q000187:drug effects; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D032442:Larrea; D009637:Masoprocol / Q000494:pharmacology; D017239:Paclitaxel / Q000494:pharmacology; D008517:Phytotherapy; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D009637:Masoprocol; D017239:Paclitaxel