Construction of a binary vector for knockout and expression analysis of rice blast fungus genes.
We developed an efficient method to analyze gene function and expression of the rice blast fungus. We constructed a GATEWAY binary vector, which generates a gene-targeted disruptant carrying a green fluorescent protein gene under the native promoter of the target gene. Using this method, the knockout efficiency and expression patterns of two hypothetical genes were determined.
Saitoh
Ken-ichiro
K
Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
Nishimura
Marie
M
Kubo
Yasuyuki
Y
Hayashi
Nagao
N
Minami
Eiichi
E
Nishizawa
Yoko
Y
D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D020082:Magnaporthe / Q000235:genetics*; D012275:Oryza / Q000382:microbiology*; D011401:Promoter Regions, Genetic / Q000235:genetics
D049452:Green Fluorescent Proteins