title: Construction of a binary vector for knockout and expression analysis of rice blast fungus genes.
abstract: We developed an efficient method to analyze gene function and expression of the rice blast fungus. We constructed a GATEWAY binary vector, which generates a gene-targeted disruptant carrying a green fluorescent protein gene under the native promoter of the target gene. Using this method, the knockout efficiency and expression patterns of two hypothetical genes were determined.
authors.affiliation: Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki 305-8602, Japan.
authors.forename: Ken-ichiro
authors.initials: K
authors.lastname: Saitoh
authors.forename: Marie
authors.initials: M
authors.lastname: Nishimura
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Kubo
authors.forename: Nagao
authors.initials: N
authors.lastname: Hayashi
authors.forename: Eiichi
authors.initials: E
authors.lastname: Minami
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nishizawa
mesh_terms: D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D049452:Green Fluorescent Proteins / Q000235:genetics; D020082:Magnaporthe / Q000235:genetics*; D012275:Oryza / Q000382:microbiology*; D011401:Promoter Regions, Genetic / Q000235:genetics
chemical_list: D049452:Green Fluorescent Proteins