Both upstream and downstream intergenic regions are critical for the mob as tumor suppressor gene activity in Drosophila.
The Drosophila mats gene plays a critical role in growth control. Using molecular genetic approaches we investigated how mats is regulated in development. A 2236-bp genomic sequence that contains entire mats including upstream and downstream intergenic regions can rescue mats mutant phenotypes, indicating that regulatory elements necessary for proper mats expression are mostly retained. However, constructs without the upstream or downstream intergenic region failed to rescue mats mutants, demonstrating the functional importance of these sequences. Moreover, mats expression is reduced in mats(e17), a mats allele with over one-third of the downstream intergenic region deleted. Consistent with a model that the downstream intergenic region is critical for mats activity, this sequence contains evolutionarily conserved elements and has enhancer activities.
Yang
Yongfei
Y
Center of Genetics and Developmental Biology, College of Life Sciences, Peking University, Beijing 100871, China.
Gupta
Vikas
V
Ho
Li-Lun
LL
Zhou
Bo
B
Fan
Qichang
Q
Zhu
Zuoyan
Z
Zhang
Wenxia
W
Lai
Zhi-Chun
ZC
D000483:Alleles; D000818:Animals; D001483:Base Sequence; D021901:DNA, Intergenic; D029721:Drosophila Proteins / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics* / Q000254:growth & development; D018507:Gene Expression Regulation, Developmental; D016147:Genes, Tumor Suppressor; D017384:Sequence Deletion; D025521:Tumor Suppressor Proteins / Q000235:genetics*
D021901:DNA, Intergenic; D029721:Drosophila Proteins; C499633:Mats protein, Drosophila; D025521:Tumor Suppressor Proteins