title: Saccharopolyspora shandongensis sp. nov., isolated from wheat-field soil.
abstract: A polyphasic study was undertaken to establish the taxonomic status of a wheat-field soil isolate. The organism, strain 88T, was found to have a range of chemical and morphological properties consistent with its classification in the genus Saccharopolyspora. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence of the strain showed that it formed a monophyletic clade with the type strain of Saccharopolyspora spinosa. However, the two strains shared low DNA-DNA relatedness. Strain 88T also had a profile of phenotypic properties that readily distinguished it from representatives of the species of Saccharopolyspora with validly published names. It is evident from the combination of genotypic and phenotypic data that the organism should be classified within a novel species of the genus Saccharopolyspora, for which the name Saccharopolyspora shandongensis sp. nov. is proposed. The type strain is strain 88T (=CGMCC 4.3530T =JCM 14614T).
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China. zhangjianli@bit.edu.cn
authors.forename: Jianli
authors.initials: J
authors.lastname: Zhang
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wu
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.forename: Zhiheng
authors.initials: Z
authors.lastname: Liu
authors.forename: Fei
authors.initials: F
authors.lastname: Song
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D020459:Genes, rRNA; D005838:Genotype; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016930:Saccharopolyspora / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D014908:Triticum
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S