Measurements of light scattering in an integrated microfluidic waveguide cytometer.
An integrated microfluidic planar optical waveguide system for measuring light scattered from a single scatterer is described. This system is used to obtain 2D side-scatter patterns from single polystyrene microbeads in a fluidic flow. Vertical fringes in the 2D scatter patterns are used to infer the location of the 90-deg scatter (polar angle). The 2D scatter patterns are shown to be symmetrical about the azimuth angle at 90 deg. Wide-angle comparisons between the experimental scatter patterns and Mie theory simulations are shown to be in good agreement. A method based on the Fourier transform analysis of the experimental and Mie simulation scatter patterns is developed for size differentiation.
Su
Xuan-Tao
XT
University of Alberta, Department of Physics, Edmonton, T6G 2G7, Canada.
Singh
Kirat
K
Capjack
Clarence
C
Petrácek
Jirí
J
Backhouse
Christopher
C
Rozmus
Wojciech
W
D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D008027:Light; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D012542:Scattering, Radiation; D018511:Systems Integration