Activation of constitutive androstane receptor under the effect of hepatocarcinogenic aminoazo dyes in mouse and rat liver.
Selective increase of DNA-binding activity of constitutive androstane receptor was detected in rat and mouse liver in response to aminoazo dyes exhibiting hepatocarcinogenic activity for these species (ortho-aminoazotoluene for mice and 3'-methyl-4-dimethylaminobenzene for rats). Competition of azo dyes with 3H-5alpha-androst-16-ene-3alpha-ol (a well-known ligand of constitutive androstane receptor) for binding to liver cell cytosol proteins was studied. Ortho-aminoazotoluene and 3'-methyl-4-dimethylaminobenzene were better competitors for cytosol proteins from mouse and rat liver, respectively.
Pakharukova
M Y
MY
Institute of Cytology and Genetics, Siberian Division of Russian Academy of Sciences, Novosibirsk. pmaria@yandex.ru
Smetanina
M A
MA
Kaledin
V I
VI
Kobzev
V F
VF
Romanova
I V
IV
Merkulova
T I
TI
D000818:Animals; D004396:Coloring Agents / Q000378:metabolism*; D000090702:Constitutive Androstane Receptor; D006801:Humans; D008024:Ligands; D008113:Liver Neoplasms / Q000139:chemically induced; D008297:Male; D008749:Methyldimethylaminoazobenzene / Q000378:metabolism*; D051379:Mice; D051381:Rats; D017208:Rats, Wistar; D018160:Receptors, Cytoplasmic and Nuclear / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D009762:o-Aminoazotoluene / Q000378:metabolism*
D004396:Coloring Agents; D000090702:Constitutive Androstane Receptor; D008024:Ligands; D018160:Receptors, Cytoplasmic and Nuclear; D014157:Transcription Factors; D008749:Methyldimethylaminoazobenzene; D009762:o-Aminoazotoluene