Technical development for production of gene-modified laboratory rats.
Transgenic rats have been used as model animals for human diseases and organ transplantation and as animal bioreactors for protein production. In general, transgenic rats are produced by pronuclear microinjection of exogenous DNA. Improvement of post-injection survival has been achieved by micro-vibration of the injection pipette. The promoter region, structural gene, chain length and strand ends of the exogenous DNA are not involved in the production efficiency of transgenic rats. Exogenous DNA prepared at 5 microg/ml seemed to be better integrated than lower and higher concentrations. Intracytoplasmic sperm injection (ICSI) has been successfully achieved in rats using a piezo-driven injection pipette. The ICSI technique has not only been applied to rescue infertile male strains but also to produce transgenic rats. The optimal DNA concentration for the ICSI-tg method (0.1 to 0.5 microg/ml) is lower than that for the conventional pronuclear microinjection. Production efficiency was improved when the membrane structure of the sperm head was partially disrupted by detergent or ultrasonic treatment before exposure to the exogenous DNA solution. For successful production of transgenic rats with a modified endogenous gene, establishment of embryonic stem cell lines or alternatively male germline stem cell lines and technical development of somatic cell nuclear transfer are still necessary for this species.
Hirabayashi
Masumi
M
National Institute for Physiological Sciences, The Graduate University for Advanced Studies, Okazaki, Japan. mhirarin@nips.ac.jp
D000818:Animals; D030801:Animals, Genetically Modified; D002460:Cell Line; D004247:DNA / Q000032:analysis / Q000378:metabolism; D046149:Embryo Culture Techniques / Q000379:methods*; D005821:Genetic Techniques; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008297:Male; D053652:Nuclear Transfer Techniques; D009865:Oocytes / Q000378:metabolism; D051381:Rats; D013045:Species Specificity; D020554:Sperm Injections, Intracytoplasmic / Q000379:methods; D013234:Stem Cells / Q000378:metabolism; D019076:Transgenes
D049452:Green Fluorescent Proteins; D004247:DNA