Overexpression of OgPAE1 from wild rice confers fungal resistance against Botrytis cinerea.
A full-length cDNA of the OgPAE1 gene encoding the alpha5 subunit of the 20S proteasome was isolated from wild rice (Oryza grandiglumis) treated by wounding or with a fungal elicitor. The deduced amino acid sequence of OgPAE1 comprises 237 amino acids (25.99 kDa), and shows 94.5% homology with Arabidopsis thaliana AtPAE1. Expression of OgPAE1 is regulated by defense-related signaling chemicals such as cantharidin, endothall and jasmonic acid. Overexpression of OgPAE1 in A. thaliana leads to resistance to the fungal pathogen Botrytis cinerea by lowering disease rate and size of necrotic lesions, and by less penetration and colonization of fungal hyphae. The results indicate that the 20S proteasome from wild rice is involved in the B. cinerea defense pathway via an as yet undetermined mechanism.
Jeon
Eun Hee
EH
Life Science and Natural Resources, Dong-A University, 840 Hadan-dong, Busan 604-714, Korea.
Chung
Eun Sook
ES
Pak
Jung Hun
JH
Nam
Jae Sung
JS
Cho
Sung Ki
SK
Shin
Sang Hyun
SH
Kim
Doh Hoon
DH
Kim
Gyung Tae
GT
Lee
Jai Heon
JH
Kang
Kyung Ho
KH
Chung
Young Soo
YS
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D020171:Botrytis / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000276:immunology*; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits
D010940:Plant Proteins; D021122:Protein Subunits