Optimization of a laboratory-developed test utilizing roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species.
Nasal and perianal swab specimens were tested for detection of Staphylococcus aureus and vancomycin-resistant Enterococcus species (VRE) using a laboratory-developed real-time PCR test and microbiological cultures. The real-time PCR and culture results for S. aureus were similar. PCR had adequate sensitivity, but culture was more specific for the detection of VRE.
Mehta
Maitry S
MS
Evanston Northwestern Healthcare, Evanston, Illinois 60201, USA.
Paule
Suzanne M
SM
Hacek
Donna M
DM
Thomson
Richard B
RB
Kaul
Karen L
KL
Peterson
Lance R
LR
D001003:Anal Canal / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D016106:Methicillin Resistance; D009666:Nose / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D020713:Vancomycin Resistance