Constitutive plasma membrane targeting and microdomain localization of Dok5 studied by single-molecule microscopy.
In the present study, single-molecule fluorescence microscopy was used to examine the characteristics of plasma membrane targeting and microdomain localization of enhanced yellow fluorescent protein (eYFP)-tagged wild-type Dok5 and its variants in living Chinese hamster ovary (CHO) cells. We found that Dok5 can target constitutively to the plasma membrane, and the PH domain is essential for this process. Furthermore, single-molecule trajectories analysis revealed that Dok5 can constitutively partition into microdomain on the plasma membrane. Finally, the potential mechanism of microdomain localization of Dok5 was discussed. This study provided insights into the characteristics of plasma membrane targeting and microdomain localization of Dok5 in living CHO cells.
Fu
Guo
G
Institute of Neuroscience, Department of Neurobiology, Second Military Medical University, Shanghai 200433, China.
Zhang
Feng
F
Cao
Li
L
Xu
Zhi-Zhan
ZZ
Chen
Yi-Zhang
YZ
Wang
Gui-Ying
GY
He
Cheng
C
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry* / Q000235:genetics; D000818:Animals; D001483:Base Sequence; D016466:CHO Cells; D002462:Cell Membrane / Q000737:chemistry*; D006224:Cricetinae; D003412:Cricetulus; D017931:DNA Primers; D008853:Microscopy / Q000379:methods*
D048868:Adaptor Proteins, Signal Transducing; D017931:DNA Primers