title: [TLR7 protein expression induced by bacterial lipopolysaccharide in DC2.4 cells].
abstract: OBJECTIVE
  To investigate the role of bacterial Lipopolysaccharide (LPS) in inducing TLR7 protein expression in DC2.4 cells.
  
  
  METHODS
  DC2.4 cells were cultured in RPMI1640 medium supplemented with 100 mL/L FBS. Optical microscope was used to observe the cells morphology before and after LPS induction. RT-PCR and Western blot were employed to analyze the expression of TLR7 at mRNA and protein lever, respectively.
  
  
  RESULTS
  Without LPS induction, DC2.4 cells were small and shine with a few dendrites, didn't express TLR7 protein. However, DC2.4 cells become bigger and the dendrites of them become more robust with LPS induction. DC2.4 cells start to express TLR7 protein after 12 hours LPS induction, and there is no much difference on TLR7 protein expression after 12 h, 24 h, 48 h LPS stimulation.
  
  
  CONCLUSIONS
  LPS can induce TLR7 protein expression in DC2.4 cells.
authors.affiliation: Center of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. shishangquansheng@yahoo.com
authors.forename: Heng-li
authors.initials: HL
authors.lastname: Yang
authors.forename: Xin-ying
authors.initials: XY
authors.lastname: Ji
authors.forename: Ping-zhong
authors.initials: PZ
authors.lastname: Wang
authors.forename: Lei
authors.initials: L
authors.lastname: Pan
authors.forename: Jian-qi
authors.initials: JQ
authors.lastname: Lian
authors.forename: Chun-qiu
authors.initials: CQ
authors.lastname: Hao
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Zhan-sheng
authors.initials: ZS
authors.lastname: Jia
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects* / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D016328:NF-kappa B / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000378:metabolism; D051197:Toll-Like Receptor 4 / Q000378:metabolism / Q000502:physiology*; D014162:Transfection
chemical_list: D008070:Lipopolysaccharides; D016328:NF-kappa B; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4; D009569:Nitric Oxide