title: Occludin immunolocalization and protein expression in goldfish.
abstract: Tight junctions (TJs) are an integral component of models illustrating ion transport mechanisms across fish epithelia; however, little is known about TJ proteins in fishes. Using immunohistochemical methods and Western blot analysis, we examined the localization and expression of occludin, a transmembrane TJ protein, in goldfish tissues. In goldfish gills, discontinuous occludin immunostaining was detected along the edges of secondary gill lamellae and within parts of the interlamellar region that line the lateral walls of the central venous sinus. In the goldfish intestine, occludin immunolocalized in a TJ-specific distribution pattern to apical regions of columnar epithelial cells lining the intestinal lumen. In the goldfish kidney, occludin was differentially expressed in discrete regions of the nephron. Occludin immunostaining was strongest in the distal segment of the nephron, moderate in the collecting duct and absent in the proximal segment. To investigate a potential role for occludin in the maintenance of the hydromineral balance of fishes, we subjected goldfish to 1, 2 and 4 weeks of food deprivation, and then examined the endpoints of hydromineral status, Na+,K+-ATPase activity and occludin protein expression in the gills, intestine and kidney. Occludin expression altered in response to hydromineral imbalance in a tissue-specific manner suggesting a dynamic role for this TJ protein in the regulation of epithelial permeability in fishes.
authors.affiliation: Department of Biology, York University, Toronto, ON, Canada, M3J 1P3. helench@yorku.ca
authors.forename: Helen
authors.initials: H
authors.lastname: Chasiotis
authors.forename: Scott P
authors.initials: SP
authors.lastname: Kelly
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D015153:Blotting, Western; D005880:Gills / Q000378:metabolism; D006054:Goldfish / Q000378:metabolism*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D062793:Occludin; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D019108:Tight Junctions / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; D062793:Occludin; D000254:Sodium-Potassium-Exchanging ATPase