title: LDL isolated from plasma-loaded red wine procyanidins resist lipid oxidation and tocopherol depletion.
abstract: Dietary phenolic compounds may act as antioxidants in vitro, but because of structural modifications during absorption, its role based on concentrations high enough to afford an antioxidant protection needs to be re-evaluated. We have explored the hypothesis that red wine procyanidins interact with low density lipoproteins (LDL) and that, at this location, the phenolic compounds efficiently protect LDL from oxidation and maintain LDL alpha-tocopherol at a high steady state concentration by recycling it back from the alpha-tocopheroxyl radical. To this end, human plasma was supplemented with wine procyanidins and isolated LDL were challenged with a constant flux of peroxyl radicals. As compared with LDL from plasma-free procyanidins, those LDL better resisted lipid oxidation and exhibited longer lag-phases of alpha-tocopherol consumption. The procyanidins, depending on their structure, were able to reduce the UV-induced alpha-tocopherol radical in a micellar system, as evidenced by electron paramagnetic ressonance. Mechanistically, the protection of LDL was interpreted in terms of quenching of peroxyl radicals and the recycling of alpha-tocopherol by the procyanidins bound to the lipoproteins. These results support the notion that, in human plasma, the procyanidins, via binding to LDL, may act as efficient local antioxidants.
authors.affiliation: Faculty of Pharmacy and Center for Neurosciences and Cell Biology, University of Coimbra, Coimbra, Portugal.
authors.forename: Cátia F
authors.initials: CF
authors.lastname: Lourenço
authors.forename: Bruno
authors.initials: B
authors.lastname: Gago
authors.forename: Rui M
authors.initials: RM
authors.lastname: Barbosa
authors.forename: Víctor
authors.initials: V
authors.lastname: de Freitas
authors.forename: João
authors.initials: J
authors.lastname: Laranjinha
mesh_terms: D005609:Free Radicals / Q000737:chemistry; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects*; D008077:Lipoproteins, LDL / Q000097:blood*; D044945:Proanthocyanidins / Q000097:blood / Q000494:pharmacology*; D014920:Wine / Q000032:analysis*; D024502:alpha-Tocopherol / Q000097:blood* / Q000737:chemistry
chemical_list: D005609:Free Radicals; D008077:Lipoproteins, LDL; D044945:Proanthocyanidins; D024502:alpha-Tocopherol