Identification and characterization of mouse Gas6 promoter.
In this study, we identify and characterize the promoter of the mouse Gas6 gene, a negative regulator of chondrogenic differentiation. We identified two highly conserved regions within the core Gas6 promoter, which are conserved among mouse, human, and rat Gas6 genes, named A-BOX and B-BOX. Basal transcriptional activity was significantly reduced after deletion of either the A-BOX or the latter half of the B-BOX. After treatment with BMP-2 for 3 days, putative A-BOX Binding Factor(s) B (ABFB) and B-BOX Binding Factor(s) (BBF) were unable to bind to their specific motifs in C3H10T1/2 cells, compared with untreated control cells. In addition, we confirmed binding of an NF-Y site within the core promoter region of mouse Gas6 gene. Taken together, these observations provide insight into the mechanism by which Gas6 is downregulated during mesenchymal stem cells differentiation into chondrocytes.
Wang
Jiayi
J
Department of Laboratory Centre, Shanghai 10th People's Hospital of Tongji University, Shanghai 200072, PR China.
Qiao
Yongxia
Y
Sun
Fenyong
F
Wan
Yang
Y
Huang
Ting
T
Ye
Tingjun
T
Yu
Yongchun
Y
D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology; D023081:CCAAT-Binding Factor / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation / Q000235:genetics; D002460:Cell Line; D019902:Chondrocytes / Q000166:cytology* / Q000378:metabolism; D020219:Chondrogenesis / Q000235:genetics*; D017124:Conserved Sequence; D004252:DNA Mutational Analysis; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000235:genetics*; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000187:drug effects; D034622:RNA Interference; D051381:Rats; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000494:pharmacology; D000099255:Growth Arrest-Specific Protein 6
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D023081:CCAAT-Binding Factor; D036341:Intercellular Signaling Peptides and Proteins; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D000099255:Growth Arrest-Specific Protein 6; C525718:BMP2 protein, human; C525719:Bmp2 protein, mouse; C525720:Bmp2 protein, rat; C492060:Nfya protein, mouse