Purified extract from Clematis mandshurica prevents adenoviral-TRAIL induced apoptosis on rat articular chondrocytes.
Since TNF-related apoptosis inducing ligand (TRAIL) is one of several apoptotic stimuli on articular chondrocytes, the modulation of the mechanism mediated by TRAIL could be considered as a novel strategy for the treatment of osteoarthritis (OA). Previous studies demonstrated that Clematis mandshurica prevents staurosporin-induced apoptosis in articular chondrocytes. This study was undertaken to examine whether Clematis mandshurica could prevent TRAIL-induced apoptosis in articular chondrocytes. Our data show that Clematis mandshurica prevents adenoviral TRAIL (Ad-TRAIL)-induced apoptosis in primary cultured articular chondrocytes. Clematis mandshurica prevents Ad-TRAIL-induced down-regulation of 14-3-3 and phosphorylated Akt. In addition, Clematis mandshurica treatment prevents the Ad-TRAIL-induced reduction of the interactions between 14-3-3 with phospho-ser112-Bad and phospho-ser136-Bad, and BcL-xL with phospho-ser155-Bad. A better understanding of the mechanism underlying inhibition of apoptosis in OA chondrocytes by Clematis mandshurica might lead to the development of a new therapeutic strategy for OA.
Lee
Sung Won
SW
Department of Rheumatology, Dong-A University College of Medicine and Institute of Medical Science, Busan, South Korea.
Lee
Hye Jeong
HJ
Moon
Jeong Bon
JB
Choi
Sun Mee
SM
Kim
Duk Kyu
DK
Kim
In Ryoung
IR
Choi
Won Chul
WC
Park
Bong Soo
BS
D048948:14-3-3 Proteins / Q000378:metabolism; D000256:Adenoviridae; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology*; D039324:Clematis; D003864:Depression, Chemical; D015536:Down-Regulation; D007596:Joints / Q000166:cytology*; D051058:Oncogene Protein v-akt / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy / Q000209:etiology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D048948:14-3-3 Proteins; D010936:Plant Extracts; D053221:TNF-Related Apoptosis-Inducing Ligand; D051058:Oncogene Protein v-akt