title: Inhibitory effects of macelignan isolated from Myristica fragrans HOUTT. on melanin biosynthesis.
abstract: The aim of this study was to investigate the in vitro inhibitory effects of macelignan isolated from Myristica fragrans HOUTT. on melanogenesis and its related enzymes such as tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2) in melan-a murine melanocytes. The IC50 values of macelignan for melanogenesis and tyrosinase were 13 microM and 30 microM, respectively, while those of arbutin as a positive control were 990 microM and 660 microM, respectively. In Western blot analysis, macelignan also significantly decreased tyrosinase, TRP-1, and TRP-2 protein expression. These results indicate that macelignan effectively inhibits melanin biosynthesis and thus could be employed as a new skin-whitening agent.
authors.affiliation: Department of Biotechnology, Yonsei University, Seoul 120-749, Korea.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Cho
authors.forename: Kyu-Hoi
authors.initials: KH
authors.lastname: Kim
authors.forename: Jae-Seok
authors.initials: JS
authors.lastname: Shim
authors.forename: Jae-Kwan
authors.initials: JK
authors.lastname: Hwang
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D007370:Interferon Type I / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D019746:Intramolecular Oxidoreductases / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D008543:Melanins / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000378:metabolism; D008544:Melanocytes / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000378:metabolism; D026323:Myristica / Q000737:chemistry*; D011257:Pregnancy Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis
chemical_list: D007370:Interferon Type I; D017705:Lignans; D008543:Melanins; D011257:Pregnancy Proteins; C020947:interferon tau; C502026:macelignan; D014442:Monophenol Monooxygenase; D019746:Intramolecular Oxidoreductases; C042501:dopachrome isomerase