Protein and DNA fingerprinting of a soil bacterial community inoculated into three different sterile soils.
The functional and genetic structures of a soil bacterial community were characterized after inoculation into three different sterile soils using a protein and DNA fingerprinting method, respectively. Principal component analysis (PCA) of profiles revealed that, depending on soil characteristics, bacterial communities with similar genetic structures harbored different functional structures and thus could potentially be of differing ecological significance for soil functioning. Co-inertia analysis between protein fingerprinting data and the corresponding sets of soil physicochemical characteristics demonstrated the correlation between the functional structure of the bacterial community and soil parameters, with pH, clay and CaCO(3) contents being the most discriminating factors.
Maron
Pierre-Alain
PA
UMR Microbiologie du Sol et de l'Environnement, INRA/Université de Bourgogne, CMSE, BP 86510, 17 rue de Sully, Dijon cedex, France. pamaron@dijon.inra.fr
Maitre
Magali
M
Mercier
Anne
A
Henri Lejon
David Pierre
DP
Nowak
Virginie
V
Ranjard
Lionel
L
D001419:Bacteria / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D016172:DNA Fingerprinting; D004591:Electrophoresis, Polyacrylamide Gel; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D001426:Bacterial Proteins; D012987:Soil