title: Structure of a 129Xe-cryptophane biosensor complexed with human carbonic anhydrase II.
abstract: Cryptophanes represent an exciting class of xenon-encapsulating molecules that can be exploited as probes for nuclear magnetic resonance imaging. The 1.70 A resolution crystal structure of a cryptophane-derivatized benezenesulfonamide complexed with human carbonic anhydrase II shows how an encapsulated xenon atom can be directed to a specific biological target. The crystal structure confirms binding measurements indicating that the cryptophane cage does not strongly interact with the surface of the protein, which may enhance the sensitivity of 129Xe NMR spectroscopic measurements in solution.
authors.affiliation: Roy and Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323, USA.
authors.forename: Julie A
authors.initials: JA
authors.lastname: Aaron
authors.forename: Jennifer M
authors.initials: JM
authors.lastname: Chambers
authors.forename: Kevin M
authors.initials: KM
authors.lastname: Jude
authors.forename: Luigi
authors.initials: L
authors.lastname: Di Costanzo
authors.forename: Ivan J
authors.initials: IJ
authors.lastname: Dmochowski
authors.forename: David W
authors.initials: DW
authors.lastname: Christianson
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D024402:Carbonic Anhydrase II / Q000737:chemistry*; D018360:Crystallography, X-Ray; D006801:Humans; D006860:Hydrogen Bonding; D046911:Macromolecular Substances / Q000737:chemistry*; D008958:Models, Molecular; D011083:Polycyclic Compounds; D014230:Triazoles / Q000737:chemistry*; D014979:Xenon Isotopes / Q000737:chemistry*
chemical_list: D046911:Macromolecular Substances; D011083:Polycyclic Compounds; D014230:Triazoles; D014979:Xenon Isotopes; C523242:cryptophane; D024402:Carbonic Anhydrase II