[Study of immunolipo-sodium morrhuate applied on human hemangioma endothelial cells in vitro].
OBJECTIVE
To explore the method of preparing immunolipo-sodium morrhuate and evaluate its effect on human hemangioma endothelial cells in vitro.


METHODS
Using SPDP((N-Succinimidyl-3-(2-pyridyldithio)) propionate) as cross-linker, anti-VEGFR2/KDR monoclone antibody was combined to the liposome surface to prepare immunolipo-sodium morrhuate by extruding method, and then its effect on human hemangioma endothelial cells in vitro was observed by laser scanning confocal microscope, inverted microscope, Gimsa staining, transmission electron microscope, MTT and flow cytometry.


RESULTS
The average diameter of the immunoliposome was 122.9 nm, which had a very good stability when compared with normal liposome, it had stronger and faster combining ability, its potential to induce apoptosis was much more prominent, and its toxic effect on human hemangioma endothelial cells was gentle, which was similar to normal liposome.


CONCLUSIONS
We have prepared immunolipo-sodium morrhuate successfully, which has very good specific initiative targetting ability in vitro and can induce pervasive apoptosis of human hemangioma endothelial cells.
Yang
Zhuang-Qun
ZQ
Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Xi'an Jiao Tong University, Xi'an, Shanxi Province, China. yzhqun@mail.xjtu.edu.cn
Song
Yong
Y
Tu
Jun-Bo
JB
Wang
Zheng-Hui
ZH
Ma
Jian-Ming
JM
Lan
Hai-Long
HL
Xu
Quan
Q
Li
Peng
P
D017209:Apoptosis / Q000187:drug effects*; D042783:Endothelial Cells; D006391:Hemangioma / Q000473:pathology*; D006801:Humans; D066298:In Vitro Techniques; D008081:Liposomes; D012597:Sclerosing Solutions / Q000494:pharmacology*; D012976:Sodium Morrhuate / Q000494:pharmacology*
D008081:Liposomes; D012597:Sclerosing Solutions; D012976:Sodium Morrhuate