Vialinin A, a novel potent inhibitor of TNF-alpha production from RBL-2H3 cells.
Thelephora vialis is a mushroom that grows in symbiosis with pine trees in Yunnan, China, a place known to have some of the richest and most diverse bioresources in the world. This is one of the most favored edible mushrooms, due to its flavor. Our screening for bioactive compounds from these mushrooms isolated a novel potent antioxidant, vialinin A, together with known compounds, from the dry fruiting bodies of T. vialis. Vialinin A is a terphenyl derivative and was elucidated by spectroscopic and chemical methods. Vialinin A showed anti-allergic activities, inhibition of beta-hexosaminidase, tumor necrosis factor (TNF)-alpha, interleukin 4 and monocyte chemotactic protein 1 release from RBL-2H3 cells, whereas atromentin and an inseparable mixture of ganbajunins D and E showed no such effects. Vialinin A displayed potent inhibition of TNF-alpha production from RBL-2H3 cells (IC50, 0.09+/-0.01 nM), indicating stronger inhibition than tacrolimus for organ transplantation (IC50, 0.25+/-0.03 nM). The potent inhibitory activities of these compounds against TNF-alpha production indicate promising new candidates for anti-allergic agents.
Onose
Jun-Ichi
J
Department of Nutritional Science, Faculty of Applied Bio-science, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan. j1onose@nodai.ac.jp
Xie
Chun
C
Ye
Yue Qi
YQ
Sugaya
Kouichi
K
Takahashi
Shunya
S
Koshino
Hiroyuki
H
Yasunaga
Katsuaki
K
Abe
Naoki
N
Yoshikawa
Kunie
K
D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D015550:Cell Degranulation / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D018932:Chemokine CCL2 / Q000378:metabolism; D016207:Cytokines / Q000096:biosynthesis; D006596:Hexosaminidases / Q000378:metabolism; D007249:Inflammation / Q000378:metabolism; D015847:Interleukin-4 / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051381:Rats; D013730:Terphenyl Compounds / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors*
C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D016207:Cytokines; D013730:Terphenyl Compounds; D014409:Tumor Necrosis Factor-alpha; C508745:vialinin A; D015847:Interleukin-4; D007770:L-Lactate Dehydrogenase; D006596:Hexosaminidases