Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO.
In this study we describe acrosome staining and motility characteristics of fresh and cryopreserved sterlet (Acipenser ruthenus L.) spermatozoa using soybean trypsin inhibitor-Alexa conjugate fluorescent staining and computer-aided sperm analysis (CASA), respectively. Methanol or dimethylsulfoxide (DMSO) were used as cryoprotectants. After cryopreservation a decline in sperm motility characteristics occurred, but no differential effect between cryoprotectant was observed. Cryopreservation caused a significant increase in the percentage of spermatozoa with acrosome stained by SBTI-Alexa for samples cryopreserved using DMSO compared to methanol. These data suggest that the low usefulness of DMSO for cryopreservation of sturgeon spermatozoa is related to its harmful specific effect towards the acrosome, probably by causing its precocious triggering, much before any egg contact.
Psenicka
Martin
M
Research Institute of Fish Culture and Hydrobiology, University of South Bohemia, 389 25 Vodnany, Czech Republic.
Dietrich
Grzegorz J
GJ
Wojtczak
Mariola
M
Nynca
Joanna
J
Rodina
Marek
M
Linhart
Otomar
O
Cosson
Jacky
J
Ciereszko
Andrzej
A
D000177:Acrosome / Q000502:physiology*; D000818:Animals; D015925:Cryopreservation; D003451:Cryoprotective Agents / Q000633:toxicity; D004121:Dimethyl Sulfoxide / Q000633:toxicity*; D005399:Fishes; D005456:Fluorescent Dyes; D008297:Male; D000432:Methanol / Q000633:toxicity*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000166:cytology / Q000502:physiology; D013194:Staining and Labeling
D003451:Cryoprotective Agents; D005456:Fluorescent Dyes; D000432:Methanol; D004121:Dimethyl Sulfoxide