The survival of heavy ion-irradiated Bcl-2 overexpressing radioresistant tumor cells and their progeny.
Here, we investigated the cell killing effectiveness of heavy-ion radiation in Bcl-2 overexpressing radioresistant tumor cells. First, irradiated cells underwent primary colony formation. Radioresistance decreased with increasing linear energy transfer (LET), indicating that heavy ions may be a promising therapeutic modality for Bcl-2 overexpressing tumors. Second, cells in primary colonies were reseeded for secondary colony formation. The incidence of delayed reproductive death increased with LET irrespective of Bcl-2 overexpression, suggesting that Bcl-2 overexpression may not facilitate heavy ion-induced genomic instability.
Hamada
Nobuyuki
N
Department of Quantum Biology, Division of Bioregulatory Medicine, Gunma University Graduate School of Medicine, Gunma 370-1292, Japan. hamada@med.gunma-u.ac.jp
Hara
Takamitsu
T
Omura-Minamisawa
Motoko
M
Funayama
Tomoo
T
Sakashita
Tetsuya
T
Sora
Sakura
S
Nakano
Takashi
T
Kobayashi
Yasuhiko
Y
D016923:Cell Death; D002470:Cell Survival; D005720:Gamma Rays; D019254:Genes, bcl-2; D006367:HeLa Cells; D020450:Heavy Ions; D006801:Humans; D018499:Linear Energy Transfer; D011836:Radiation Tolerance; D011839:Radiation, Ionizing