[Comparative analysis of molecular genetic studies using DNA samples isolated from blood treated with EDTA or heparin].
A molecular genetic investigation of five genes: CFTR, GSTM1, GCTT1, MTHFR, and TP53 using DNA samples isolated from blood treated with either EDTA or stabilized heparin was conducted. Twenty DNA samples obtained from 10 persons. The results prove that heparin used as an anticoagulant instead of EDTA had no inhibitory effect on PCR and PCR/RFLP results, at least when DNA was isolated with a DNA-sorb-B kit. The results suggest that heparin-treated blood samples can be also used to study other genes.
Markova
E V
EV
Kaz'mina
N V
NV
Zotova
N V
NV
D000925:Anticoagulants / Q000494:pharmacology*; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000235:genetics; D004247:DNA / Q000097:blood / Q000235:genetics* / Q000302:isolation & purification*; D004492:Edetic Acid / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000235:genetics; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D016159:Tumor Suppressor Protein p53 / Q000235:genetics
D000925:Anticoagulants; D019005:Cystic Fibrosis Transmembrane Conductance Regulator; D004247:DNA; D004492:Edetic Acid; D005982:Glutathione Transferase; D006493:Heparin; D016159:Tumor Suppressor Protein p53; C505032:CFTR protein, human; C495901:TP53 protein, human