Non-enzymatic glycation of chondrocyte-seeded collagen gels for cartilage tissue engineering.
Collagen glycated with ribose (250 mM) in solution (pre-glycation) and as a gel (post-glycation) was seeded with chondrocytes and the effects of glycation on chondrocyte matrix assembly in culture were determined. Pre-glycation enhanced GAG accumulation significantly over controls at both 2 and 4 weeks (p < 0.05), although at both time points there were no statistical differences in cell number between pre-glycated and control gels. The increased proteoglycan accumulation was shown to be in part due to significantly increased GAG retention by the pre-glycated constructs (p < 0.05). Total collagen content in these pre-glycated gels was also significantly higher than unglycated gels at 4 weeks (p < 0.05). With post-glycation of collagen gels, chondrocyte number and GAG accumulation were all significantly lower than controls (p < 0.05). Post-glycation also inhibited GAG retention by the constructs (p < 0.05). Given these results, pre-glycation may be an improved processing method for collagen gels for tissue engineering techniques.
Roy
Rani
R
Sibley School of Mechanical and Aerospace Engineering, Cornell University, 218 Upson Hall, Ithaca, New York 14853, USA.
Boskey
Adele L
AL
Bonassar
Lawrence J
LJ
D000818:Animals; D002417:Cattle; D002452:Cell Count; D049109:Cell Proliferation; D019902:Chondrocytes / Q000737:chemistry / Q000166:cytology / Q000378:metabolism*; D024042:Collagen Type I / Q000378:metabolism*; D019245:Compressive Strength; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism*; D005782:Gels; D006025:Glycosaminoglycans / Q000737:chemistry / Q000378:metabolism; D006031:Glycosylation; D051381:Rats; D012266:Ribose / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*
D024042:Collagen Type I; D005782:Gels; D006025:Glycosaminoglycans; D012266:Ribose