[Biocompatibility of combined deproteinized bone coated with hepatocyte growth factor as scaffold for osteoblasts in vitro in fetal rabbits].
OBJECTIVE
To determine the cellular compatibility of combined deproteinized bone(DPB) coated with hepatocyte growth factor (HGF), and to observe the adherent effect of osteoblasts in response to HGF.


METHODS
Osteoblasts were isolated from fetal rabbits. Osteoblasts were cultured with DPB coated with HGF and deproteinized bone as experimental group and contral group, respectively. The proliferation and alkalinephosphatase activity were tested. Their growth was examined by inverted phase contrast microscope and scanning electronmicroscope.


RESULTS
The osteoblasts were attached to the outside and inside surfaces and grew well. HGF/DPB could stimulate the alkalinephosphatase activity of the osteoblasts and improve the proliferation of the osteoblasts.


CONCLUSIONS
HGF/DPB has good biocompatibility and bone induction. HGF could improve the adherent effect of DPB on osteoblasts, and it could be used as scaffold material for the bone tissue engineering.
Dang
Hong-sheng
HS
Department of Orthopaedics, Taihe Hospital, Medical School of Yunyang, Shiyan Hubei, China. danghsh@tom.com
Zhao
Meng
M
Yan
Yong-xiang
YX
Liu
Jia-guo
JG
D000818:Animals; D001672:Biocompatible Materials / Q000494:pharmacology*; D018786:Bone Substitutes / Q000378:metabolism; D001842:Bone and Bones / Q000166:cytology; D049109:Cell Proliferation; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D011247:Pregnancy; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D001672:Biocompatible Materials; D018786:Bone Substitutes; D017228:Hepatocyte Growth Factor