A study on a tissue-engineered bone using rhBMP-2 induced periosteal cells with a porous nano-hydroxyapatite/collagen/poly(L-lactic acid) scaffold.
We investigated the in vivo osteogenic ability of rhBMP-2 induced periosteal cells in a new porous scaffold, nano-hydroxyapatite (nano-HA)/collagen/poly(L-lactic acid) (PLA). The nano-HA/collagen/PLA composites were utilized as an extracellular matrix for a cell-based strategy of bone tissue engineering. Periosteal cells were cultivated with 500 ng ml(-1) rhBMP-2, followed by seeding into prewet nano-HA/collagen/PLA scaffolds. The cell-scaffold constructs were then subcutaneously implanted in nude mice compared to controls with cell suspension and scaffold alone. Scanning electron microscopy examination proved that the scaffold supported adhesion and proliferation of periosteal cells. Histological bone formation was observed only in experimental groups with cell transplants 8 weeks post-implantation. The animals of the control groups did not show bone formation. The results strongly encourage the approach of the transplantation of rhBMP-2 induced periosteal cells within a suitable carrier structure for bone regeneration.
Zhang
Chao
C
Department of Orthopaedics, Navy General Hospital of PLA, Beijing 100037, People's Republic of China. zcmail2002@yahoo.com.cn
Hu
Yun-Yu
YY
Cui
Fu-Zhai
FZ
Zhang
Shu-Ming
SM
Ruan
Di-Ke
DK
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001846:Bone Development / Q000187:drug effects / Q000502:physiology; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000008:administration & dosage* / Q000737:chemistry; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D008819:Mice, Nude; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D010012:Osteogenesis / Q000187:drug effects / Q000502:physiology*; D010521:Periosteum / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation*; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D011817:Rabbits; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000737:chemistry; D023822:Tissue Engineering / Q000379:methods*; D016212:Transforming Growth Factor beta / Q000008:administration & dosage* / Q000737:chemistry
D001672:Biocompatible Materials; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D004337:Drug Carriers; D011091:Polyesters; D011108:Polymers; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; C499469:recombinant human bone morphogenetic protein-2; D019344:Lactic Acid; C033616:poly(lactide); D003094:Collagen; D017886:Durapatite