A method for identification of vaginal epithelial microflora by the analysis of 16S ribosomal RNA gene.
Analysis of 16S ribosomal RNA gene was carried out in 2 DNA samples isolated from vaginal epithelial smears from women suffering from bacterial vaginosis. The composition of vaginal epithelial microbiocenosis in bacterial vaginosis was determined and its significant difference from normocenosis was shown. A laboratory protocol for identification of vaginal epithelial microflora was developed on the basis of 16S ribosomal RNA gene analysis.
Larin
A K
AK
Institute of Physicochemical Medicine, Federal Agency for Health Care and Social Development, Moscow. zelaz@yandex.ru
Seleznyova
O V
OV
Momynaliev
K T
KT
D001419:Bacteria / Q000235:genetics; D004848:Epithelium / Q000382:microbiology*; D005260:Female; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D014621:Vagina / Q000033:anatomy & histology / Q000382:microbiology; D016585:Vaginosis, Bacterial / Q000382:microbiology
D012336:RNA, Ribosomal, 16S