Detection of SHV-1 beta-lactamase in Pseudomonas aeruginosa strains by genetic methods.
Twelve multidrug-resistant Pseudomonas aeruginosa (MDRPA) isolates were recovered over a period of two years in the National Bone Marrow Transplant Centre of Tunisia. MDRPA isolates were isolated from seven patients and from three environmental samples. Isoelectric focusing revealed pIs of 8.2, 5.5 and 7.6 in all MDRPA isolates. These strains produced the OXA-18 extended spectrum beta-lactamase and an SHV type beta-lactamase as shown by screening PCR analysis. DNA hybridization confirmed this inference, detecting bla(SHV) gene in these isolates. Pulsed-field gel electrophoresis (PFGE) defined one predominant genomic group; group A (seven isolates) and four different genotypes containing one to two isolates. Clonally related isolates were recovered from three patients and from two washbasins. Sequencing DNA of cluster representative strains identified the classical bla(SHV-1) gene. For these strains, the nucleotide sequence of the structural bla(SHV-1) gene was nearly identical to those previously described. Such enzyme has not been reported from P. aeruginosa. This is the first report of the SHV-1 penicillinase in epidemic P. aeruginosa strain.
Kalai Blagui
S
S
Service des laboratoires, centre national de greffe de moelle osseuse de Tunis, rue Djebel-Lakdhar-Bab-Saadoun, 1006 Tunis, Tunisia.
Achour
W
W
Bejaoui
M
M
Abdeladhim
A
A
Ben Hassen
A
A
D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial; D018432:Drug Resistance, Multiple; D016521:Electrophoresis, Gel, Pulsed-Field; D004755:Enterobacteriaceae / Q000201:enzymology; D006801:Humans; D007525:Isoelectric Focusing; D007709:Klebsiella / Q000201:enzymology; D008826:Microbial Sensitivity Tests; D010405:Penicillinase / Q000235:genetics / Q000302:isolation & purification; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D001618:beta-Lactamases / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D017931:DNA Primers; D004269:DNA, Bacterial; D010405:Penicillinase; C054882:beta-lactamase PIT-2; D001618:beta-Lactamases