title: Hyperbaric oxygen therapy effects on tissue lesions in acute pancreatitis. Experimental study in rats.
abstract: OBJECTIVE
  To study the effects of hyperbaric oxygen therapy on tissue lesions in an experimental model of acute pancreatitis induced by pancreatic duct ligation.
  
  
  METHODS
  Forty-eight adult female Wistar rats were randomized into two groups (n=24): control group and hyperbaric oxygen therapy group.
  
  
  METHODS
  The second group was treated with a two-hour daily session of hyperbaric oxygen therapy at 2.5 ATA started 6 hours after pancreatic duct ligation.
  
  
  METHODS
  The two groups were divided into 3 subgroups of 8 rats each undergoing euthanasia on days 1, 3, and 7 after the acute pancreatitis induction.
  
  
  METHODS
  The pancreas was evaluated according to the following histopathologic criteria: edema, hemorrhage, acinar necrosis and leukocyte infiltration.
  
  
  RESULTS
  Hyperbaric oxygen therapy was efficient in significantly reducing acinar necrosis on the first day (P=0.049) and the foci of hemorrhage on the seventh day (P=0.050). The edema and leukocyte infiltration did not show the expected reduction.
  
  
  CONCLUSIONS
  The utilization of a daily session of hyperbaric oxygen therapy at 2.5 ATA is efficient in reducing the hemorrhage and acinar necrosis but is not sufficient to reduce edema and leukocyte infiltration.
authors.affiliation: Laboratory of Physiology, Center of Biological Sciences, University of Caxias do Sul (UCS), Caxias do Sul, RS, Brazil.
authors.forename: Marcio
authors.initials: M
authors.lastname: Festugato
authors.forename: Celso Picolli
authors.initials: CP
authors.lastname: Coelho
authors.forename: Gustavo
authors.initials: G
authors.lastname: Fiedler
authors.forename: Felipe Pioner
authors.initials: FP
authors.lastname: Machado
authors.forename: Mauro Cabral
authors.initials: MC
authors.lastname: Gonçalves
authors.forename: Filipe Rech
authors.initials: FR
authors.lastname: Bassani
authors.forename: Paulo Henrique
authors.initials: PH
authors.lastname: Pierezan
authors.forename: Alessandro Bersch
authors.initials: AB
authors.lastname: Osvaldt
authors.forename: Luiz
authors.initials: L
authors.lastname: Rohde
mesh_terms: D000208:Acute Disease; D000681:Amylases / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006470:Hemorrhage / Q000175:diagnosis; D006931:Hyperbaric Oxygenation; D009336:Necrosis / Q000175:diagnosis; D010195:Pancreatitis / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D012107:Research Design
chemical_list: D000681:Amylases