Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos.
Human embryonic stem (hES) cells are self-renewing, pluripotent cells that are valuable research tools and hold promise for use in regenerative medicine. Most hES cell lines are derived from cryopreserved human embryos that were created during in vitro fertilization (IVF) and are in excess of clinical need. Embryos that are discarded during the IVF procedure because of poor morphology and a low likelihood for generating viable pregnancies or surviving the cryopreservation process are also a viable source of hES cells. In this protocol, we describe how to derive novel hES cells from discarded poor-quality embryos and how to maintain the hES cell lines.
Lerou
Paul H
PH
Division of Newborn Medicine, Brigham & Women's Hospital and Children's Hospital Boston, Karp Family Research Building 7214, 300 Longwood Avenue, Boston, Massachusetts 02115, USA.
Yabuuchi
Akiko
A
Huo
Hongguang
H
Miller
Justine D
JD
Boyer
Leah F
LF
Schlaeger
Thorsten M
TM
Daley
George Q
GQ
D053624:Blastocyst Inner Cell Mass / Q000166:cytology*; D018929:Cell Culture Techniques / Q000379:methods*; D053595:Embryonic Stem Cells / Q000166:cytology*; D005307:Fertilization in Vitro; D006801:Humans; D014015:Tissue Banks