Phenolic composition of Cynara cardunculus L. organs, and their biological activities.
Polyphenols are bioactive molecules exhibiting a lot of scientific attention due to their multiple biological activities. This study compared phenolic contents and antioxidant activity in Cynara cardunculus L. organs and focus on leaf phenolic compounds identification by RP-HPLC and their antibacterial activity. The analyzed organs exhibited different total polyphenol contents (7-14.8 mg GAE g(-1) DW). Leaf and seed phenolic contents were similar and two times higher than those in flowers. The same tendency was observed for the amount of flavonoids and tannins. However, seed extracts displayed the highest DPPH. scavenging ability with the lowest IC50 value (23 microg ml(-1)), followed by leaves and flowers (over 50 microg ml(-1)). In contrast, leaves showed the highest capacity to quench superoxide (IC50: 1 microg ml(-1)) as compared to seeds (6 microg ml(-1)). In addition, cardoon leaves were efficient to inhibit growth of pathogenic bacteria mainly against Staphylococcus aureus and Escherichia coli. The identification of phenolic compounds from leaves revealed that syringic and trans-cinnamic acids were the major molecules.
Falleh
Hanen
H
Laboratoire d'adaptation des plantes aux stress abiotiques, Centre de biotechnologie à la technopole de Borj-Cédria (CBBC), BP 901, 2050 Hammam-Lif, Tunisia.
Ksouri
Riadh
R
Chaieb
Kamel
K
Karray-Bouraoui
Najoua
N
Trabelsi
Najla
N
Boulaaba
Mondher
M
Abdelly
Chedly
C
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D031942:Cynara / Q000737:chemistry / Q000378:metabolism*; D005419:Flavonoids / Q000032:analysis / Q000096:biosynthesis; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D005839:Gentamicins / Q000494:pharmacology; D000432:Methanol; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000032:analysis / Q000378:metabolism*; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry / Q000378:metabolism; D012997:Solvents; D013481:Superoxides / Q000378:metabolism; D013634:Tannins / Q000032:analysis / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D005839:Gentamicins; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D013634:Tannins; D013481:Superoxides; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol