title: Alteration of expression of Ca2+ signaling proteins and adaptation of Ca2+ signaling in SERCA2+/- mouse parotid acini.
abstract: OBJECTIVE
  The sarco/endoplasmic reticulum Ca2+-ATPase (SERCA), encoded by ATP2A2, is an essential component for G-protein coupled receptor (GPCR)-dependent Ca2+ signaling. However, whether the changes in Ca2+ signaling and Ca2+ signaling proteins in parotid acinar cells are affected by a partial loss of SERCA2 are not known.
  
  
  METHODS
  In SERCA2+/- mouse parotid gland acinar cells, Ca2+ signaling, expression levels of Ca2+ signaling proteins, and amylase secretion were investigated.
  
  
  RESULTS
  SERCA2+/- mice showed decreased SERCA2 expression and an upregulation of the plasma membrane Ca2+ ATPase. A partial loss of SERCA2 changed the expression level of 1, 4, 5-tris-inositolphosphate receptors (IP3Rs), but the localization and activities of IP3Rs were not altered. In SERCA2+/- mice, muscarinic stimulation resulted in greater amylase release, and the expression of synaptotagmin was increased compared to wild type mice.
  
  
  CONCLUSIONS
  These results suggest that a partial loss of SERCA2 affects the expression and activity of Ca2+ signaling proteins in the parotid gland acini, however, overall Ca2+ signaling is unchanged.
authors.affiliation: Department of Oral Medicine, Yonsei University College of Dentistry, 250 Seongsanno, Seodaemun-gu, Seoul 120-752, Korea.
authors.forename: Jong-Hoon
authors.initials: JH
authors.lastname: Choi
authors.forename: Hae
authors.initials: H
authors.lastname: Jo
authors.forename: Jeong Hee
authors.initials: JH
authors.lastname: Hong
authors.forename: Syng-Ill
authors.initials: SI
authors.lastname: Lee
authors.forename: Dong Min
authors.initials: DM
authors.lastname: Shin
mesh_terms: D000681:Amylases / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D002217:Carbachol / Q000494:pharmacology; D007150:Immunohistochemistry; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D010306:Parotid Gland / Q000378:metabolism*; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000502:physiology
chemical_list: D053496:Inositol 1,4,5-Trisphosphate Receptors; D002217:Carbachol; D000681:Amylases; D053498:Sarcoplasmic Reticulum Calcium-Transporting ATPases; D002118:Calcium