title: Identification of the L,D-transpeptidases for peptidoglycan cross-linking in Escherichia coli.
abstract: Three active-site cysteine L,D-transpeptidases can individually anchor the Braun lipoprotein to the Escherichia coli peptidoglycan. We show here that two additional enzymes of the same family form peptide bonds between the third residues of peptidoglycan stems, generating meso-DAP(3)-->meso-DAP(3) unusual cross-links. This activity partially replaces the D,D-transpeptidase activity of penicillin-binding proteins.
authors.affiliation: INSERM U872-LRMA, Equipe 12, Centre de Recherche des Cordeliers, 15 Rue de l'Ecole de Médecine, 75270 Paris, Cedex 06, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Magnet
authors.forename: Lionel
authors.initials: L
authors.lastname: Dubost
authors.forename: Arul
authors.initials: A
authors.lastname: Marie
authors.forename: Michel
authors.initials: M
authors.lastname: Arthur
authors.forename: Laurent
authors.initials: L
authors.lastname: Gutmann
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism*; D008956:Models, Chemical; D046915:Penicillin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry / Q000378:metabolism; D010458:Peptidyl Transferases / Q000235:genetics / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; D010458:Peptidyl Transferases