title: Correlation between CLSI, EUCAST and Etest methodologies for amphotericin B and fluconazole antifungal susceptibility testing of Candida spp. clinical isolates.
abstract: This study analyzed the correlation between the results obtained through two microdilution methods: Clinical and Laboratory Standard Institute (CLSI) (M27-A2) and European Committee on Antibiotic Susceptibility Testing (EUCAST) (document E. Dis. 7.1) and an agar base method Etest for determining minimmun inhibitory concentration (MIC) for amphotericin B and fluconazole against 30 clinical isolates of Candida spp. The agreement between Etest, CLSI and EUCAST MICs within +/- 2 log2 dilutions was higher for amphotericin B than for fluconazole However, Pearson correlation demonstrated a greater agreement for fluconazole. The categorical agreement between MICs provided by the Etest/ CLSI and Etest/EUCAST methodologies was high for both amphotericin B (100%) and fluconazole (> or = 96.66%). This study demonstrated the adequacy of Etest method using Mueller Hinton agar to evaluate amphotericin B and fluconazole susceptibility of clinical isolates of Candida spp.
authors.affiliation: Departamento de Ciências Biológicas, Universidade Federal de Alfena (UNIFAL-MG), Alfenas-MG, Brazil.
authors.forename: A L R
authors.initials: AL
authors.lastname: Claudino
authors.forename: R F
authors.initials: RF
authors.lastname: Peixoto
authors.forename: M S C
authors.initials: MS
authors.lastname: Melhem
authors.forename: M W
authors.initials: MW
authors.lastname: Szeszs
authors.forename: J P
authors.initials: JP
authors.lastname: Lyon
authors.forename: J K
authors.initials: JK
authors.lastname: Chavasco
authors.forename: M C
authors.initials: MC
authors.lastname: Franco
mesh_terms: D000362:Agar; D000666:Amphotericin B / Q000494:pharmacology* / Q000592:standards*; D000935:Antifungal Agents / Q000494:pharmacology* / Q000592:standards*; D002175:Candida / Q000187:drug effects*; D002177:Candidiasis / Q000382:microbiology; D003470:Culture Media; D004058:Diffusion; D015725:Fluconazole / Q000494:pharmacology* / Q000592:standards*; D008826:Microbial Sensitivity Tests / Q000592:standards
chemical_list: D000935:Antifungal Agents; D003470:Culture Media; D000666:Amphotericin B; D015725:Fluconazole; D000362:Agar