title: CD133+ cells from human peripheral blood promote corticospinal axon regeneration.
abstract: To assess the effect of endothelial progenitor cells on the axon growth of cortex neurons, we transplanted CD133 cells derived from human peripheral blood to an organotypic coculture system consisting of spinal cord and cortex from neonatal rats. The axon growth from cortex to spinal cord was significantly promoted in cultures after CD133 cells transplantation compared with that of the control cultures. In addition, real-time reverse transcription-PCR showed a significant upregulation of vascular endothelial growth factor mRNA in the spinal cord of the cultures containing CD133 cells. In contrast, the transplanted cells did not differentiate into endothelial cells. These data suggest that CD133 cells may promote axonal regeneration by upregulating vascular endothelial growth factor mRNA in spinal cord tissues.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Japan. riyamamoto@hiroshima-u.ac.jp
authors.forename: Risako
authors.initials: R
authors.lastname: Yamamoto
authors.forename: Masakazu
authors.initials: M
authors.lastname: Ishikawa
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Tanaka
authors.forename: Naosuke
authors.initials: N
authors.lastname: Kamei
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Nakanishi
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Sasaki
authors.forename: Toshio
authors.initials: T
authors.lastname: Nakamae
authors.forename: Yu
authors.initials: Y
authors.lastname: Mochizuki
authors.forename: Takayuki
authors.initials: T
authors.lastname: Asahara
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000071916:AC133 Antigen; D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000378:metabolism*; D001369:Axons / Q000502:physiology*; D002454:Cell Differentiation / Q000502:physiology; D002540:Cerebral Cortex / Q000166:cytology*; D018920:Coculture Techniques; D006023:Glycoproteins / Q000378:metabolism*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D009416:Nerve Regeneration / Q000502:physiology*; D009924:Organ Culture Techniques; D010455:Peptides / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology*; D015854:Up-Regulation / Q000502:physiology; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics
chemical_list: D000071916:AC133 Antigen; D015703:Antigens, CD; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; C000606025:Prom1 protein, rat; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; C467498:vascular endothelial growth factor A, rat