Wide-ranging genomic effects of plasticisers and related compounds.
The effects of four compounds, bis(2-ethylhexyl)phthalate (BEHP); diisodecylphthalate (DIP); 4-n-octylphenol (OP); 4-chloro-3-methylphenol (CMP), on gene expression (steady-state mRNA levels) across the whole human genome were studied in human TE671 cells. Effects were studied using the Affymetrics GeneChip Human Genome U133 Plus 2.0, HG-U133 Plus 2.0 arrays, The array analyses the expression of 47,000 transcripts and variants, including approximately 38,500 well characterised. All four compounds exerted statistically significant actions, affecting between 4 and 6.5% of all genes. Each compound had its own expression signature. In most instances where there was an effect, steady-state mRNA levels were decreased, although not always. CMP treatment caused most increases in mRNA levels. A mixture of DIP and CMP caused fewer changes in mRNA levels than either of the individual compounds.


CONCLUSIONS
These plasticisers affected the steady-state mRNA levels of many human genes. Exposure to these compounds over many years has the potential to influence human health.
Turan
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N
Department of Medicine, Division of Medical Sciences, The Medical School, University of Birmingham, UK.
Cartwright
L S
LS
Waring
R H
RH
Ramsden
D B
DB
D019921:Biotinylation; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D004247:DNA / Q000096:biosynthesis / Q000235:genetics; D017931:DNA Primers / Q000494:pharmacology; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D003850:Deoxyribonuclease I / Q000096:biosynthesis / Q000235:genetics; D004785:Environmental Pollutants / Q000633:toxicity*; D023281:Genomics; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D010968:Plasticizers / Q000633:toxicity*; D011061:Poly A / Q000235:genetics; D018075:RNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects
D017931:DNA Primers; D018076:DNA, Complementary; D004785:Environmental Pollutants; D010968:Plasticizers; D018075:RNA, Complementary; D012333:RNA, Messenger; D011061:Poly A; D004247:DNA; D003850:Deoxyribonuclease I