Fast and simple method for the simultaneous evaluation of the capacity and efficiency of food antioxidants in trapping peroxyl radicals in an intestinal model system.
A simple oxygraphic method, for which the theoretical and experimental bases have been recently revised, has been successfully applied to evaluate the peroxyl radical chain-breaking characteristics of some typical food antioxidants in micelle systems, among which is a system that reproduces conditions present in the upper part of the digestive tract, where the absorption and digestion of lipids occur. This method permits one to obtain from a single experimental run the peroxyl radical trapping capacity (PRTC, that is, the number of moles of peroxyl radicals trapped by a given amount of food), the peroxyl radical trapping efficiency (PRTE, that is, the reciprocal of the amount of food that reduces to half the steady-state concentration of peroxyl radicals), and the half-life of the antioxidant ( t(1/2)) when only a small fraction of peroxyl radicals reacts with the antioxidants present in foods. Examples of application of the method to various types of foodstuffs have been reported, assessing the general validity of the method in the simple and fast evaluation of the above-reported fundamental antioxidant characteristics of foods.
Rossetto
Monica
M
Department of Biological Chemistry, University of Padova, viale G. Colombo 3, 35131 Padova, Italy.
Vanzani
Paola
P
De Marco
Veronica
V
Zennaro
Lucio
L
Scarpa
Marina
M
Rigo
Adelio
A
D000975:Antioxidants / Q000737:chemistry*; D005504:Food Analysis; D016166:Free Radical Scavengers / Q000737:chemistry; D007422:Intestines / Q000737:chemistry*; D015227:Lipid Peroxidation; D008954:Models, Biological; D010545:Peroxides / Q000737:chemistry*
D000975:Antioxidants; D016166:Free Radical Scavengers; D010545:Peroxides; C049375:perhydroxyl radical