[Effects of genistein on the expressions of cell apoptosis-related proteins in salivary adenoid cystic carcinoma cell line SACC-83].
OBJECTIVE
To investigate the molecular mechanism of cell apoptosis induced by tyrosine protein kinase inhibitor, genistein, in human salivary adenoid cystic carcinoma cell line SACC-83.


METHODS
SACC-83 cells cultured in vitro were treated with genistein, the expressions of bax, bcl-2 and survivin proteins were detected with Western blotting, and the results were quantitatively analyzed by FluorChem V2.0 software, statistical analysis was performed with analysis of variance using SPSS11.5 software package.


RESULTS
With the increase of concentration of genistein and the elongation of time, the expression of bax protein was significantly increased, but the expression of bcl-2 and survivin proteins was significantly decreased. Treated with 220 micromol/L genistein for 3 days, the expression level of bax protein was 3.43 folds of the control group (P<0.01),but the expression level of bcl-2 and survivin proteins was respectively 85%(P<0.05) and 35%(P<0.01) of the control group.


CONCLUSIONS
The cell apoptosis induced by genistein in human salivary adenoid cystic carcinoma cell line SACC-83 may be associated with the upregulation of bax protein expression,and the downregulations of bcl-2 and survivin proteins expression.
Ma
Jie
J
Department of Oral Medicine, Shengjing Hospital of China Medical University, Shenyang, China. zgbl_2003@163.com
Wang
Jie
J
Zhong
Ming
M
Wang
Zhao-Yuan
ZY
D017209:Apoptosis / Q000187:drug effects; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism*; D002460:Cell Line; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology*; D006801:Humans; D011506:Proteins / Q000378:metabolism; D012468:Salivary Gland Neoplasms / Q000378:metabolism*
D011506:Proteins; D019833:Genistein