title: Carbon-protein covalent conjugates in non-instrumental immunodiagnostic systems.
abstract: An original technique for obtaining stable conjugates using colloid carbon particles as indicator labels has been developed. The reliability and stability of the diagnostic reagents obtained is provided by covalent binding of various affine compounds on the surface of carbon particles. The stability of the reagents has been studied under various storage conditions for 3-10 years. It was shown that even when storage conditions were outside the optimal range, some conjugates preserved their analytical characteristics for 10 years. A number of systems for analytical detection of various ligands have been designed based on the carbon-protein conjugates synthesized. The major characteristics of these systems are their high sensitivity and specificity, reliability, and reproducibility, simplicity in operation, and quick results.
authors.affiliation: Institute of Ecology and Genetics of Microorganisms UB RAS, 13 Golev Street, Perm, 614081, Russia.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Rayev
authors.forename: Konstantin
authors.initials: K
authors.lastname: Shmagel
mesh_terms: D002244:Carbon / Q000737:chemistry*; D006063:Chorionic Gonadotropin / Q000302:isolation & purification; D003102:Colloids; D005260:Female; D015483:HIV Antibodies / Q000097:blood; D015658:HIV Infections / Q000097:blood / Q000821:virology; D015497:HIV-1 / Q000276:immunology / Q000302:isolation & purification; D015498:HIV-2 / Q000276:immunology / Q000302:isolation & purification; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*; D008297:Male; D009419:Nerve Tissue Proteins / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry*
chemical_list: D006063:Chorionic Gonadotropin; D003102:Colloids; C029152:G-substrate; D015483:HIV Antibodies; D009419:Nerve Tissue Proteins; D002244:Carbon; D019809:Streptavidin