title: Safety and immunogenicity of myxoma virus as a new viral vector for small ruminants.
abstract: Myxoma virus (MYXV), a leporide-specific poxvirus, represents an attractive candidate for the generation of safe and non-replicative vaccine vectors for other species. With the aim of developing new recombinant vaccines for ruminants, we evaluated the safety and the immunogenicity of recombinant MYXV in sheep. In vitro studies indicated that ovine primary fibroblasts were not permissive for MYXV and that infection of ovine peripheral blood mononuclear cells occurred at a low rate. Although non-specific activation significantly improved the susceptibility of lymphocytes, MYXV infection remained abortive. Histological and immunohistochemical examination at the inoculation sites revealed the development of an inflammatory process and allowed the detection of sparse infected cells in the dermis. In addition, inoculated sheep developed an antibody response directed against MYXV and the product of the transgene. Overall, these results provide the first line of evidence on the potential of MYXV as a viral vector for ruminants.
authors.affiliation: Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Pignolet
authors.affiliation: Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Boullier
authors.affiliation: Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Gelfi
authors.affiliation: Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Marjorie
authors.initials: M
authors.lastname: Bozzetti
authors.affiliation: AFSSA LERPRA les Templiers, 105 route des Chappes, F-06902 Sophia Antipolis, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Russo
authors.affiliation: Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Eliane
authors.initials: E
authors.lastname: Foulon
authors.affiliation: Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Meyer
authors.affiliation: Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Maxence
authors.initials: M
authors.lastname: Delverdier
authors.affiliation: Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Gilles
authors.initials: G
authors.lastname: Foucras
authors.affiliation: Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Bertagnoli
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D005347:Fibroblasts / Q000821:virology; D005822:Genetic Vectors / Q000008:administration & dosage / Q000502:physiology*; D017929:Hemorrhagic Disease Virus, Rabbit / Q000276:immunology; D007271:Injections, Intradermal; D007963:Leukocytes, Mononuclear / Q000821:virology; D009233:Myxoma virus / Q000472:pathogenicity / Q000502:physiology*; D011817:Rabbits; D016865:Reassortant Viruses / Q000502:physiology; D012756:Sheep; D012867:Skin / Q000821:virology; D013045:Species Specificity; D014611:Vaccination / Q000379:methods* / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage; D014774:Virulence; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus