[Protective effect of insulin on oxygen-radical induced hepatic injury in severely scalded rats].
OBJECTIVE
To investigate the protective effect of insulin on oxygen-radical induced hepatic injury in severely scalded rats in early stage of severe scald.


METHODS
Eighty-four male Sprague-Dawley rats were randomly divided into three groups: i. e, normal group, saline group, and insulin group, with 28 rat in each group. The rats in the latter two groups were subjected to 30% TBSA full-thickness scald on the back, and received intra-peritoneal injection of 40ml/kg isotonic saline, and subcutaneous injection of 3 IU/kg insulin, respectively. The total anti-oxygen capability (T-AOC), the expression of superoxide dismutase (SOD), reactive oxygen species (ROS) and intercellular adhesion molecule (ICAM-1) in hepatic tissue, and serum alanine transaminase (ALT) were determined in each group at 6, 12, 24, 48 post-scald hours (PSH) with corresponding methods.


RESULTS
The hepatic T-AOC and SOD content were obviously decreased, while the ROS content were markedly increased at 6 PSH in saline group compared with that in normal group (P < 0.05 or P < 0.01). The expression of ICAM-1 and serum content of ALT were significantly higher than that in normal group at 12 PSH and 48 PSH (P < 0.01). At 24 PSH, the hepatic T-AOC (386 +/- 75) U/g and SOD content (210 +/- 39 ) U/g were obviously higher in insulin group than those in saline group [(124 +/- 18), (111 +/- 9) U/g, respectively, P < 0.01), but the ROS content (154 +/- 29 ) U/g was much lower than that in saline group [(351 +/- 41) U/g, respectively, P < 0.01]. At 48 PSH, the serum content of ALT and hepatic expression of ICAM-1 in insulin group exhibited obvious difference when compared with those in saline group (P < 0.01). Meanwhile, Pathological examination showed that hepatic injury was alleviated by insulin administration after scald.


CONCLUSIONS
Insulin administration early after severe scald exhibits protective effect on liver function by improving anti-oxygen radical ability of rat liver.
Wang
Yun-Chuan
YC
Burn Center of PLA, Xijing Hospital, Fourth Military Medical University, Xián 710032, PR China.
Jia
Chi-Yu
CY
Zhang
Wan-Fu
WF
Lv
Gen-Fa
GF
Dong
Mao-Long
ML
Tang
Chao-Wu
CW
Hu
Da-Hai
DH
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D002056:Burns / Q000378:metabolism* / Q000473:pathology; D007328:Insulin / Q000494:pharmacology*; D008099:Liver / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
D007328:Insulin; D017382:Reactive Oxygen Species; D013482:Superoxide Dismutase; D000410:Alanine Transaminase