DNA microarray gene expression profile of marginal zone versus follicular B cells and idiotype positive marginal zone B cells before and after immunization with Streptococcus pneumoniae.
Marginal zone (MZ) B cells play an important role in the clearance of blood-borne bacterial infections via rapid T-independent IgM responses. We have previously demonstrated that MZ B cells respond rapidly and robustly to bacterial particulates. To determine the MZ-specific genes that are expressed to allow for this response, MZ and follicular (FO) B cells were sort purified and analyzed via DNA microarray analysis. We identified 181 genes that were significantly different between the two B cell populations. Ninety-nine genes were more highly expressed in MZ B cells while 82 genes were more highly expressed in FO B cells. To further understand the molecular mechanisms by which MZ B cells respond so rapidly to bacterial challenge, Id-positive and -negative MZ B cells were sort purified before (0 h) or after (1 h) i.v. immunization with heat-killed Streptococcus pneumoniae, R36A, and analyzed via DNA microarray analysis. We identified genes specifically up-regulated or down-regulated at 1 h following immunization in the Id-positive MZ B cells. These results give insight into the gene expression pattern in resting MZ vs FO B cells and the specific regulation of gene expression in Ag-specific MZ B cells following interaction with Ag.
Kin
Nicholas W
NW
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294.
Crawford
Dianna M
DM
Center for Immunology, Department of Medicine, University of Minnesota, Minneapolis, MN 55455.
Liu
Jiabin
J
Center for Immunology, Department of Medicine, University of Minnesota, Minneapolis, MN 55455.
Behrens
Timothy W
TW
Center for Immunology, Department of Medicine, University of Minnesota, Minneapolis, MN 55455.
Kearney
John F
JF
Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294.
D000818:Animals; D016175:B-Lymphocyte Subsets / Q000276:immunology*; D001402:B-Lymphocytes / Q000276:immunology*; D015153:Blotting, Western; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D018858:Germinal Center / Q000276:immunology*; D007130:Immunoglobulin Idiotypes / Q000235:genetics / Q000276:immunology; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D011008:Pneumococcal Infections / Q000276:immunology*; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D013296:Streptococcus pneumoniae / Q000276:immunology
D007130:Immunoglobulin Idiotypes; D012333:RNA, Messenger