Maintenance of the intestinal tube in Caenorhabditis elegans: the role of the intermediate filament protein IFC-2.
The Caenorhabditis elegans intestinal lumen is surrounded by a dense cytoplasmic network that is laterally attached to the junctional complex and is referred to as the endotube. It localizes to the terminal web region which anchors the microvillar actin filament bundles and is particularly rich in intermediate filaments. To examine their role in intestinal morphogenesis and function, C. elegans reporter strains were generated expressing intestine-specific CFP-tagged intermediate filament polypeptide IFB-2. When these animals were treated with dsRNA against intestinal intermediate filament polypeptide IFC-2, the endotube developed multiple bubble-shaped invaginations that protruded into the enterocytic cytoplasm. The irregularly widened lumen remained surrounded by a continuous IFB-2::CFP-labeled layer. Comparable but somewhat mitigated phenotypic changes were also noted in wild-type N2 worms treated with ifc-2 (RNAi). Junctional complexes were ultrastructurally and functionally normal and the apical domain of intestinal cells was also not altered. These observations demonstrate that IFC-2 is important for structural maintenance of the intestinal tube but is not needed for establishment of the endotube and epithelial cell polarity.
Hüsken
Katrin
K
Department of Anatomy and Cell Biology, Johannes Gutenberg-University, Mainz, Germany.
Wiesenfahrt
Tobias
T
Abraham
Christian
C
Windoffer
Reinhard
R
Bossinger
Olaf
O
Leube
Rudolf E
RE
D000818:Animals; D017173:Caenorhabditis elegans / Q000166:cytology / Q000502:physiology*; D029742:Caenorhabditis elegans Proteins / Q000502:physiology* / Q000648:ultrastructure; D016764:Cell Polarity / Q000235:genetics / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology; D018507:Gene Expression Regulation, Developmental / Q000502:physiology; D006706:Homeostasis / Q000502:physiology; D007381:Intermediate Filament Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D007413:Intestinal Mucosa / Q000378:metabolism*; D007422:Intestines / Q000166:cytology* / Q000648:ultrastructure; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission
D029742:Caenorhabditis elegans Proteins; C533714:IFC-2 protein, C elegans; D007381:Intermediate Filament Proteins