title: Chloramphenicol-incorporated poly lactide-co-glycolide (PLGA) nanoparticles: formulation, characterization, technetium-99m labeling and biodistribution studies.
abstract: Chloramphenicol-loaded (CHL) poly-d,l-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) were prepared by emulsification solvent evaporation technique either by using polyvinyl alcohol (PVA) as emulsion stabilizer or polysorbate-80 (PS-80) as surfactant and characterised by transmission electron microscopy, zeta-potential measurements. The NPs were radiolabeled with technetium-99m ((99m)Tc) by stannous reduction method. Labeling conditions were optimised to achieve high-labeling efficiency, in vitro and in vivo (serum) stability. The labeled complexes also showed very low transchelation as determined by DTPA challenge test. Biodistribution studies of (99m)Tc-labeled complexes were performed after intravenous administration in mice. The CHL-loaded PLGA NPs coated with PS-80 exhibited relatively high brain uptake with comparatively low accumulation in bone marrow to that of free drug and CHL-loaded PLGA NPs (PVA, used as emulsion stabilizer) at 24 h post injection time period. This indicates the usefulness of the above delivery system for prolonged use of the antibiotic.
authors.affiliation: Nuclear Medicine Division, Indian Institute of Chemical Biology, Kolkata, India.
authors.forename: Kamal Krishna
authors.initials: KK
authors.lastname: Halder
authors.forename: Bivash
authors.initials: B
authors.lastname: Mandal
authors.forename: Mita Chatterjee
authors.initials: MC
authors.lastname: Debnath
authors.forename: Hriday
authors.initials: H
authors.lastname: Bera
authors.forename: Lakshmi Kanto
authors.initials: LK
authors.lastname: Ghosh
authors.forename: Bijon Kumar
authors.initials: BK
authors.lastname: Gupta
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D002614:Chelating Agents / Q000737:chemistry; D055598:Chemical Phenomena; D002626:Chemistry, Pharmaceutical; D002627:Chemistry, Physical; D002701:Chloramphenicol / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology*; D004355:Drug Stability; D004563:Electrochemistry; D006863:Hydrogen-Ion Concentration; D007275:Injections, Intravenous; D007553:Isotope Labeling; D019344:Lactic Acid; D051379:Mice; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D004369:Pentetic Acid / Q000737:chemistry; D011100:Polyglycolic Acid; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013667:Technetium; D017971:Tin Compounds / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D000900:Anti-Bacterial Agents; D002614:Chelating Agents; D017971:Tin Compounds; C023599:stannous chloride; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D002701:Chloramphenicol; D013667:Technetium; D004369:Pentetic Acid