Highly efficient galvanotaxis apparatus for cleaning and concentrating rumen ciliates.
Galvanotaxis was shown to be an efficient method for cleaning and concentrating rumen ciliate protozoa whose harvesting (centrifugation of large volumes of in vitro cultures followed by repeated washing of the sediment to remove plant debris) is time consuming. We suggested the use of a new galvanotaxis apparatus (a small-capacity two-way glass stopcock) to improve cell yield in concentrating the rumen ciliate protozoa and cleaning them from impurities. Migration of the ciliates (Entodinium caudatum, Entodinium furca monolobum and Diploplastron affine) into the cathode compartment under different electric currents (0, 5, 10, and 15 mA) and intervals (5, 10, 20, and 30 min) was evaluated. The lethal current level was 20 mA. Cell yield was 9-81%, depending on ciliate species, migration time and current. The migration time significantly affected both E. caudatum and D. affine. The electric current-migration time interplay was shown to be significant in both E. caudatum and D. affine. The advantages and disadvantages of the tested apparatus were determined; the two-way glass stopcock was very convenient for both cleaning and concentrating rumen ciliate in vitro cultures by galvanotaxis.
Kisidayová
S
S
Institute of Animal Physiology, Slovak Academy of Sciences, 040 01 Kosice, Slovakia. kisiday@saske.sk
Váradyová
Z
Z
Mihaliková
K
K
D000818:Animals; D002465:Cell Movement; D002470:Cell Survival; D016798:Ciliophora / Q000302:isolation & purification*; D004560:Electricity; D008828:Microbiological Techniques / Q000379:methods*; D012417:Rumen / Q000469:parasitology*; D013997:Time Factors