[Protection of cadaver lungs of non-heart-beating donor lung transplantation in rats].
OBJECTIVE
To investigate the methods to alleviate lung injury of non-heart-beating donor to attain better structure and function.


METHODS
Sixty-four Sprague-Dawley rats were randomly divided into 4 groupsú a heart-beating donor group(HBD group), a non-heart-beating donor group without protective measures(NHBD-N group), a non-heart-beating donor group with continuous mechanical ventilation or with topical cooling on cadaver lung (NHBD-V group and NHBD-I group). After the transplantation, lung compliance,pulmonary function,wet/dry ratio of lung and content of energy metabolism were compared among the 4 groups.


RESULTS
Due to the longer warm ischemic period, NHBD lungs suffered more injuries than HBD lungs. However, compared with NHBD-N group, the wet/dry ratio of the lung in NHBD-V group and NHBD-I group was lower(5.28+/-1.24,4.21+/-0.85,4.14+/-1.33,P<0.01),the lung injury index (14.35+/-3.21,11.28+/-3.26,10.41+/-2.85, P<0.01)and the count of white blood cells(425.60+/-86.47,316.30+/-56.24,295.50+/-70.26, P<0.01) were milder, while the lung compliance and preservation of energetic metabolte were better in the NHBD-V group and the NHBD-I group.


CONCLUSIONS
Continuous mechanical ventilation or topical cooling may protect the NHBD lung during the warm ischemic period.
Hu
Qing-hua
QH
Department of Cardiothoracic Surgery, Xiangya Hospital, Central South University, Changsha, China.
Chen
Sheng-xi
SX
Luo
Fan-yan
FY
Zhu
Guo-yong
GY
D000818:Animals; D002102:Cadaver; D006323:Heart Arrest / Q000378:metabolism / Q000503:physiopathology; D008168:Lung / Q000378:metabolism* / Q000503:physiopathology; D008170:Lung Compliance; D016040:Lung Transplantation / Q000379:methods*; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014019:Tissue Donors