Quantitative SERRS for DNA sequence analysis.
SERRS is an extremely sensitive and selective technique which when applied to the detection of labelled DNA sequences allows detection limits to be obtained which rival, and in most cases are better than, fluorescence. In this tutorial review the conditions are explored which enable the successful detection of DNA using SERRS. The enhancing surface which is used is crucial and in this case suspensions of nanoparticles were the focus as they allow quantitative behaviour to be achieved in systems analogous to current fluorescence based approaches. The aggregation conditions required to obtain SERRS of DNA affect the sensitivity and the reproducibility and we describe the use of spermine as an effective aggregating agent to achieve excellent reproducibility and sensitivity. The nature of the label which is used, be it fluorescent or non-fluorescent, positively or negatively charged, also affects the SERRS response and these conditions are again discussed. Finally, we show how to detect a specific target DNA sequence in a meaningful diagnostic assay using SERRS and how the approaches described previously in the review are vital to the success of such approaches.
Graham
Duncan
D
Centre for Molecular Nanometrology, WestCHEM, Department of Pure and Applied Chemistry, 295 Cathedral Street, Glasgow, UK. duncan.graham@strath.ac.uk
Faulds
Karen
K
D004247:DNA / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000379:methods; D008855:Microscopy, Electron, Scanning; D015335:Molecular Probes / Q000737:chemistry*; D017422:Sequence Analysis, DNA; D013059:Spectrum Analysis, Raman / Q000379:methods
D015335:Molecular Probes; D004247:DNA