A high through-put reverse genetic screen identifies two genes involved in remote memory in mice.
Previous studies have revealed that the initial stages of memory formation require several genes involved in synaptic, transcriptional and translational mechanisms. In contrast, very little is known about the molecular and cellular mechanisms underlying later stages of memory, including remote memory (i.e. 7-day memory). To identify genes required for remote memory, we screened randomly selected mouse strains harboring known mutations. In our primary reverse genetic screen, we identified 4 putative remote memory mutant strains out of a total of 54 lines analyzed. Additionally, we found 11 other mutant strains with other abnormal profiles. Secondary screens confirmed that mutations of integrin beta2 (Itgbeta2) and steryl-O-acyl transferase 1 (Soat1) specifically disrupted remote memory. This study identifies some of the first genes required for remote memory, and suggests that screens of targeted mutants may be an efficient strategy to identify molecular requirements for this process.
Matynia
Anna
A
Department of Neurobiology, University of California Los Angeles, Los Angeles, California, United States of America.
Anagnostaras
Stephan G
SG
Wiltgen
Brian J
BJ
Lacuesta
Maress
M
Fanselow
Michael S
MS
Silva
Alcino J
AJ
D000818:Animals; D004597:Electroshock; D005820:Genetic Testing / Q000379:methods*; D008568:Memory; D008570:Memory, Short-Term; D051379:Mice / Q000235:genetics*; D009154:Mutation; D015203:Reproducibility of Results; D013997:Time Factors