Cell-cycle control of microRNA-mediated translation regulation.
MicroRNAs are small regulatory RNA molecules that exert post-transcriptional control overexpression of specific target mRNAs. AU-rich elements (AREs) are highly conserved 3'UTR sequences that alter the stability and translation of mRNAs of clinical importance as a rapid and transient response to external and internal changes. We recently demonstrated that a reporter mRNA containing the tumor necrosis factor alpha (TNFalpha) ARE activates translation in response to quiescence via microRNA target sites in the ARE. Further studies revealed that microRNAs in general have the potential to regulate translation in a cell cycle determined manner: in quiescent cells, microRNAs activate translation while in cycling/proliferating cells, microRNAs repress translation.
Vasudevan
Shobha
S
Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06536, USA.
Tong
Yingchun
Y
Steitz
Joan A
JA
D020413:3' Untranslated Regions / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000378:metabolism*; D041681:NIH 3T3 Cells; D014176:Protein Biosynthesis / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism*; D016196:S Phase / Q000502:physiology*
D020413:3' Untranslated Regions; D035683:MicroRNAs; D012333:RNA, Messenger