Anti-tumor activities of andrographolide, a diterpene from Andrographis paniculata, by inducing apoptosis and inhibiting VEGF level.
Andrographolide (1), a diterpenoid lactone isolated from a traditional herb (Andrographis paniculata), is known to possess potent anti-inflammatory activity. In this study, we investigated the anti-tumor effect of 1 on various cancer cell lines in vitro. It induced apoptosis of prostate cancer (PC-3) cells (the most sensitive cell line among the cell lines screened) via the activation of caspase 3, up-regulation of bax, and down-regulation of bcl-2. Furthermore, its inhibitory activity on the level of vascular endothelial growth factor was also verified by ELISA.
Zhao
Feng
F
School of Pharmacy, Yantai University, Yantai, China. zhaofeng@ytu.edu.cn
He
En-Qi
EQ
Wang
Lu
L
Liu
Ke
K
D035685:Andrographis / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004587:Electrophoresis, Agar Gel; D006801:Humans; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors*; D051028:bcl-2-Associated X Protein / Q000378:metabolism
D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D042461:Vascular Endothelial Growth Factor A; D051028:bcl-2-Associated X Protein; C030419:andrographolide; D053148:Caspase 3