Highly sensitive detection of pharmaceutical compounds in biological fluids using capillary electrophoresis coupled with laser-induced native fluorescence.
Due to its selectivity and sensitivity, capillary electrophoresis coupled to laser-induced fluorescence has evolved as a useful analytical tool for determining drugs in biological samples. This paper describes a simple, sensitive, efficient, and rapid method for analyzing propranolol without derivatization in plasma by CE-LIF using a diode solid-state laser at 266nm. An experimental design methodology was assessed for the investigation of electrophoretic parameters and a Box-Behnken design was selected to optimize both sensitivity and efficiency. The CE-LIF method was linear over the concentration range of 50-850ngmL(-1) and successfully applied to a real forensic sample.
Schappler
Julie
J
Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences - EPGL, University of Geneva, University of Lausanne, Bd d'Yvoy 20, 1211 Geneva 4, Switzerland.
Staub
Aline
A
Veuthey
Jean-Luc
JL
Rudaz
Serge
S
D001826:Body Fluids / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007834:Lasers; D004364:Pharmaceutical Preparations / Q000032:analysis* / Q000097:blood; D010949:Plasma / Q000737:chemistry; D011433:Propranolol / Q000032:analysis / Q000097:blood; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013696:Temperature
D004364:Pharmaceutical Preparations; D011433:Propranolol