Repolarization heterogeneity in the right ventricular outflow tract: correlation with ventricular arrhythmias in Brugada patients and in an in vitro canine Brugada model.
BACKGROUND
Brugada syndrome (BrS) is characterized by repolarization abnormality with ST-segment elevation in the right ventricular outflow tract (RVOT).


OBJECTIVE
Although action potential (AP) heterogeneity is associated with induction of ventricular arrhythmias (VA) in BrS, clinical evidence and its experimental correlations are still absent and are the focus of this study.


METHODS
We evaluated repolarization heterogeneity in 15 patients with BrS using body surface mapping and in 8 pairs of isolated canine RVOT and right ventricular anteroinferior (RVAI) preparations having drug-induced BrS using optical mapping.


RESULTS
Patients had large J-ST-segment elevation and long QT interval in the RVOT at baseline. Administration of pilsicainide (1 mg/kg) exaggerated J-ST-segment elevation, caused simultaneous long and short QT intervals in the RVOT, and induced polymorphic ventricular tachycardia (VT) and T wave alternans (TWA). Dispersion of QT within the RVOT after pilsicainide was greater in patients that had syncope or ventricular fibrillation than those that did not. Ventricular arrhythmias originated from the RVOT along with local electrocardiogram changes and TWA. Repolarization heterogeneity was much less in areas outside the RVOT. Inducing BrS increased AP heterogeneity (with and without spike-and-dome) within the RVOT epicardium. Phase 2 reentry and TWA originated from the epicardium in 88% and 50% of RVOT preparations, respectively. In contrast, the RVOT endocardium and RVAI had little AP heterogeneity, with neither reentry nor TWA.


CONCLUSIONS
The instability and heterogeneity of repolarization within the epicardium of the RVOT seem to be associated with arrhythmogenesis in both patients and in the in vitro tissue models of BrS.
Morita
Hiroshi
H
Krannert Institute of Cardiology, Indiana University School of Medicine, Indianapolis, Indiana, USA. hmorita@cc.okayama-u.ac.jp
Zipes
Douglas P
DP
Fukushima-Kusano
Kengo
K
Nagase
Satoshi
S
Nakamura
Kazufumi
K
Morita
Shiho T
ST
Ohe
Tohru
T
Wu
Jiashin
J
D000200:Action Potentials; D000328:Adult; D000818:Animals; D018780:Body Surface Potential Mapping; D053840:Brugada Syndrome / Q000175:diagnosis* / Q000235:genetics / Q000503:physiopathology*; D004195:Disease Models, Animal; D004285:Dogs; D018740:Genetic Heterogeneity; D006329:Heart Conduction System / Q000503:physiopathology; D006352:Heart Ventricles / Q000503:physiopathology*; D006801:Humans; D008297:Male; D008875:Middle Aged; D017180:Tachycardia, Ventricular / Q000175:diagnosis* / Q000503:physiopathology*