DigesTip: a new device for a rapid and efficient in-solution protein digestion.
DigesTip is a new device for in-solution protein digestion, based on a patent pending technology, able to immobilize enzymes (trypsin, in this case) on a solid surface, keeping their activity preserved. DigesTip is a standard pipette tip, usable both by human and by robots. Its main performances are: very short digestion time (1 min) and usability with low protein sample concentrations (5 microg/mL). DigesTip obtains a clear signal in MS measurements and its usage rules out several preparative steps.
Marangoni
Roberto
R
ProteoGen Bio S.r.l., Pontedera, Pisa, Italy. marangon@di.unipi.it
Chiarini
Riccardo
R
Iannone
Giorgio
G
Salerno
Massimiliano
M
D001709:Biotechnology / Q000379:methods; D004867:Equipment Design; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007221:Industry; D007700:Kinetics; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000737:chemistry; D011506:Proteins / Q000737:chemistry; D040901:Proteomics / Q000379:methods*; D012709:Serum Albumin / Q000737:chemistry; D013696:Temperature; D013997:Time Factors; D014357:Trypsin / Q000737:chemistry
D010455:Peptides; D011506:Proteins; D012709:Serum Albumin; D014357:Trypsin