title: Hymenobacter psychrotolerans sp. nov., isolated from the Qinghai--Tibet Plateau permafrost region.
abstract: A psychrotolerant bacterium, designated strain Tibet-IIU11T, was isolated from the Qinghai--Tibet Plateau permafrost region, China. A taxonomic study was conducted using a polyphasic approach, with determination of physiological and biochemical properties and phylogenetic analysis based on 16S rRNA gene sequences. The novel isolate was found to belong to the genus Hymenobacter and was distinct from the recognized species of this genus. The major fatty acids were iso-C15 : 0 (24.5 %), summed feature 3 (C16 : 1omega7c and/or iso C15 : 0 2-OH; 20.0 %), summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B; 14.0 %), C16 : 1omega5c (12.7 %) and anteiso-C15 : 0 (8.4 %). Phosphatidylethanolamine and an unknown aminophospholipid were predominant in the polar lipid profile. The quinone system consisted exclusively of menaquinone MK-7 and sym-homospermidine was the major polyamine present. These chemotaxonomic traits are in good agreement with the characteristics of the genus Hymenobacter. The assignment of the novel isolate to this genus was further supported by a DNA G+C content of 60 mol%. On the basis of the polyphasic evidence presented in this study, it is proposed that strain Tibet-IIU11T (=DSM 18569T=CGMCC 1.6365T) represents a novel species, Hymenobacter psychrotolerans sp. nov.
authors.affiliation: School of Life Sciences, Key Lab of Arid and Grassland Agroecology of MOE, Lanzhou University, Lanzhou 730000, PR China.
authors.forename: Gaosen
authors.initials: G
authors.lastname: Zhang
authors.forename: Fujun
authors.initials: F
authors.lastname: Niu
authors.forename: Hans-Jürgen
authors.initials: HJ
authors.lastname: Busse
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Maoxing
authors.initials: M
authors.lastname: Dong
authors.forename: Huyuan
authors.initials: H
authors.lastname: Feng
authors.forename: Lizhe
authors.initials: L
authors.lastname: An
authors.forename: Guodong
authors.initials: G
authors.lastname: Cheng
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D003080:Cold Temperature; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S