Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules.
Synthesis of ribosomal RNAs (rRNAs) is the major transcriptional event in proliferating cells. In eukaryotes, ribosomal DNA (rDNA) is transcribed by RNA polymerase I from a multicopy locus coexisting in at least two different chromatin states. This heterogeneity of rDNA chromatin has been an obstacle to defining its molecular composition. We developed an approach to analyze differential protein association with each of the two rDNA chromatin states in vivo in the yeast Saccharomyces cerevisiae. We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1.
Merz
Katharina
K
Universitaet Regensburg, Institut für Biochemie, Genetik und Mikrobiologie, 93053 Regensburg, Germany.
Hondele
Maria
M
Goetze
Hannah
H
Gmelch
Katharina
K
Stoeckl
Ulrike
U
Griesenbeck
Joachim
J
D015139:Blotting, Southern; D002843:Chromatin / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D004275:DNA, Ribosomal / Q000235:genetics / Q000378:metabolism*; D005363:Ficusin / Q000737:chemistry; D018628:Gene Dosage; D020459:Genes, rRNA / Q000235:genetics; D006609:High Mobility Group Proteins / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008957:Models, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012269:Ribosomal Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D002843:Chromatin; D003432:Cross-Linking Reagents; D004271:DNA, Fungal; D004275:DNA, Ribosomal; C103154:HMO1 protein, S cerevisiae; D006609:High Mobility Group Proteins; D006657:Histones; D012269:Ribosomal Proteins; D029701:Saccharomyces cerevisiae Proteins; D005363:Ficusin