title: Efficient and direct fermentation of starch to ethanol by sake yeast strains displaying fungal glucoamylases.
abstract: Aspergillus oryzae glucoamylases encoded by glaA and glaB, and Rhizopus oryzae glucoamylase, were displayed on the cell surface of sake yeast Saccharomyces cerevisiae GRI-117-UK and laboratory yeast S. cerevisiae MT8-1. Among constructed transformants, GRI-117-UK/pUDGAA, displaying glaA glucoamylase, produced the most ethanol from liquefied starch, although MT8-1/pUDGAR, displaying R. oryzae glucoamylase, had the highest glucoamylase activity on its cell surface.
authors.affiliation: Research Institute, Gekkeikan Sake Co., Ltd., Fushimi-ku, Kyoto 612-8385, Japan. a_kotaka@gekkeikan.co.jp
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kotaka
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sahara
authors.forename: Yoji
authors.initials: Y
authors.lastname: Hata
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Abe
authors.forename: Akihiko
authors.initials: A
authors.lastname: Kondo
authors.forename: Michiko
authors.initials: M
authors.lastname: Kato-Murai
authors.forename: Kouichi
authors.initials: K
authors.lastname: Kuroda
authors.forename: Mitsuyoshi
authors.initials: M
authors.lastname: Ueda
mesh_terms: D001236:Aspergillus oryzae / Q000201:enzymology; D000431:Ethanol / Q000378:metabolism*; D005285:Fermentation; D005087:Glucan 1,4-alpha-Glucosidase / Q000378:metabolism*; D012233:Rhizopus / Q000201:enzymology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D014920:Wine / Q000382:microbiology*
chemical_list: D000431:Ethanol; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase