title: Multispectral optical imaging device for in vivo detection of oral neoplasia.
abstract: A multispectral digital microscope (MDM) is designed and constructed as a tool to improve detection of oral neoplasia. The MDM acquires in vivo images of oral tissue in fluorescence, narrow-band (NB) reflectance, and orthogonal polarized reflectance (OPR) modes, to enable evaluation of lesions that may not exhibit high contrast under standard white light illumination. The device rapidly captures image sequences so that the diagnostic value of each modality can be qualitatively and quantitatively evaluated alone and in combination. As part of a pilot clinical trial, images are acquired from normal volunteers and patients with precancerous and cancerous lesions. In normal subjects, the visibility of vasculature can be enhanced by tuning the reflectance illumination wavelength and polarization. In patients with histologically confirmed neoplasia, we observe decreased blue/green autofluorescence and increased red autofluorescence in lesions, and increased visibility of vasculature using NB and OPR imaging. The perceived lesion borders change with imaging modality, suggesting that multimodal imaging has the potential to provide additional diagnostic information not available using standard white light illumination or by using a single imaging mode alone.
authors.affiliation: Rice University, Department of Bioengineering, 6100 Main St., Houston, Texas 77251-1892, USA. dmr2@rice.edu
authors.forename: Darren
authors.initials: D
authors.lastname: Roblyer
authors.forename: Rebecca
authors.initials: R
authors.lastname: Richards-Kortum
authors.forename: Konstantin
authors.initials: K
authors.lastname: Sokolov
authors.forename: Adel K
authors.initials: AK
authors.lastname: El-Naggar
authors.forename: Michelle D
authors.initials: MD
authors.lastname: Williams
authors.forename: Cristina
authors.initials: C
authors.lastname: Kurachi
authors.forename: Ann M
authors.initials: AM
authors.lastname: Gillenwater
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D003936:Diagnosis, Computer-Assisted / Q000379:methods*; D006801:Humans; D009062:Mouth Neoplasms / Q000175:diagnosis* / Q000378:metabolism*; D010783:Photometry / Q000379:methods*; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013057:Spectrum Analysis / Q000379:methods*
chemical_list: D014408:Biomarkers, Tumor