title: [Effect of G-CSF on the proliferation and differentiation of bcr/abl(+)-CD34+ cells from CML patients].
abstract: OBJECTIVE
  To study the effect of granulocyte colony-stimulating factor (G-CSF) on the proliferation and differentiation of bcr/abl(+)-CD34+ cells.
  
  
  METHODS
  bcr/abl(+)-CD34+ cells were isolated from bone marrow of chronic myelocytic leukemia (CML) patients and were treated with 0, 10, 100, 1000 ng/ml of G-CSF for 48, 96, 144 hs. CD34 cells from normal bone marrow were used as controls. Cell proliferation was determined by trypan blue dye exclusion, cell-cycle and antigen differentiation were determined by flow cytometry and cell morphology was observed under light microscope.
  
  
  RESULTS
  The number of bcr/abl(+)-CD34+ cells was increased obviously in all groups. After cultured for 48 and 96 h, the number of bcr/abl(+)-CD34+ cells at G-CSF 10 ng/ml group was significantly higher than that in G-CSF 0 ng/ml group (P < 0.05) , the number of normal CD34 cells was increased only in the presence of G-CSF. After cultured for 48, 96 and 144 h, the cell number in G-CSF 100 ng/ml group was significantly higher than that in G-CSF 0 ng/ml group (P < 0.05, P < 0.01, P < 0.01, respectively). After cultured for 144 h, the cell percentages in G0/G1 phase for bcr/abl(+)-CD34+ cells in G-CSF 10, 100, 1000 ng/ml groups were significantly less than that in G-CSF 0 ng/ml group (P < 0. 05), and that for normal CD34 cells in G-CSF 10, 100, 1000 ng/ml groups were significantly less than that of G-CSF 0 ng/ml group after cultured for 48 and 96 h. The expressions of CD34 on bcr/abl(+)-CD34+ cells and normal CD34+ cells were decreased along with the culture duration, accompanied by the expression of CD33 and CD13 increased first and decreased later, which was not correlated with the concentration of G-CSF. Both bcr/abl(+)-CD34+ cells and normal CD34+ cells showed mature morphology along with proliferation and differentiation.
  
  
  CONCLUSIONS
  G-CSF promotes proliferation of both bcr/abl(+)-CD34+ cells and normal CD34+ cells, but not necessary for the former, and the former differentiates more rapidly than the latter does, but both was independent of G-CSF.
authors.affiliation: Department of Hematology, Nanfang Hospital, Nanfang Medical University, Guangzhou, China.
authors.forename: Cheng-Yu
authors.initials: CY
authors.lastname: Li
authors.forename: Fan-Yi
authors.initials: FY
authors.lastname: Meng
authors.forename: Qi-Xin
authors.initials: QX
authors.lastname: Sun
authors.forename: Yun-Bi
authors.initials: YB
authors.lastname: Fu
authors.forename: Qian-Li
authors.initials: QL
authors.lastname: Jiang
authors.forename: Zheng-Shan
authors.initials: ZS
authors.lastname: Yi
authors.forename: Lan-Lin
authors.initials: LL
authors.lastname: Song
mesh_terms: D000328:Adult; D000368:Aged; D018952:Antigens, CD34 / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D016044:Fusion Proteins, bcr-abl / Q000378:metabolism; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009000:Monocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology; D014407:Tumor Cells, Cultured
chemical_list: D018952:Antigens, CD34; D016179:Granulocyte Colony-Stimulating Factor; D016044:Fusion Proteins, bcr-abl