title: Macrophage physiological function after superparamagnetic iron oxide labeling.
abstract: Our goal was to analyze the changes in morphology and physiological function (phagocytosis, migratory capabilities, humoral and cellular response, and nitric oxide secretion) of murine macrophages after labeling with a clinically used superparamagnetic iron oxide (SPIO), ferucarbotran. In SPIO-treated macrophages, nanoparticles were taken up in the cytoplasm and accumulated in a membrane-bound organelle. Macrophage proliferation and viability were not modified after SPIO labeling. Phagocytic function decreased after labeling with only 10 microg Fe/mL SPIO, whereas other functions including migration and production of tumor necrosis factor-alpha and nitric oxide increased at the highest SPIO concentration (100 microg Fe/mL).
authors.affiliation: Institute of Biomedical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Jong-Kai
authors.initials: JK
authors.lastname: Hsiao
authors.forename: Hung-Hao
authors.initials: HH
authors.lastname: Chu
authors.forename: Yu-Hsiu
authors.initials: YH
authors.lastname: Wang
authors.forename: Chih-Wei
authors.initials: CW
authors.lastname: Lai
authors.forename: Pi-Tai
authors.initials: PT
authors.lastname: Chou
authors.forename: Sung-Tsang
authors.initials: ST
authors.lastname: Hsieh
authors.forename: Jaw-Lin
authors.initials: JL
authors.lastname: Wang
authors.forename: Hon-Man
authors.initials: HM
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D048429:Cell Size; D002470:Cell Survival; D003287:Contrast Media; D005290:Ferric Compounds; D008264:Macrophages / Q000166:cytology* / Q000502:physiology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008280:Magnetics; D051379:Mice; D010587:Phagocytosis / Q000502:physiology*; D013194:Staining and Labeling / Q000379:methods
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; C000499:ferric oxide