title: [Rapid determination of medical components found in the health food for weight loss by liquid chromatography/tandem mass spectrometry (LC/MS/MS)].
abstract: A method for the rapid determination of 11 medical components found in health foods for weight loss using liquid chromatography/tandem mass spectrometry (LC/MS/MS) has been developed. HPLC separation is performed on an ODS column with the gradient elution method. The mobile phase consists of two solvents. Solvent A is water and solvent B is methanol/acetonitrile (1:1), and both contain 0.1% formic acid and 5 mmol/l of ammonium acetate. Each medical component is analyzed with multiple-reaction monitoring (MRM) in both negative and positive modes through electrospray ionization (ESI). The recovery rates of the 11 medical components added to commercially available health foods were 46.3-114% and each coefficient of variation was 13.7% or less. It was confirmed that this method is applicable to the urgent analysis of health foods that have caused damage to health.
authors.affiliation: Hiroshima Prefectural Technology Research Institute, Health and Environment Center, Minami-ku, Hiroshima, Japan. h-date81371@pref.hiroshima.lg.jp
authors.forename: Hideyo
authors.initials: H
authors.lastname: Date
authors.forename: Akie
authors.initials: A
authors.lastname: Toyota
authors.forename: Masahiro
authors.initials: M
authors.lastname: Terauchi
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Sugimura
authors.forename: Takeshi
authors.initials: T
authors.lastname: Matsuo
authors.forename: Chieko
authors.initials: C
authors.lastname: Mochiike
mesh_terms: D000097:Acetonitriles; D019440:Anti-Obesity Agents / Q000009:adverse effects / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D058870:Food, Organic / Q000009:adverse effects / Q000032:analysis*; D000432:Methanol; D012997:Solvents; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014867:Water
chemical_list: D000097:Acetonitriles; D019440:Anti-Obesity Agents; D012997:Solvents; D014867:Water; D000432:Methanol; C032159:acetonitrile