Design and synthesis of biotinylated inositol phosphates relevant to the biotin-avidin techniques.
Six bifunctional molecules containing biotin and various inositol phosphates were synthesized. These compounds were designed on the basis of X-ray structures of the complexes of D-myo-inositol 1,4,5-triphosphates (IP(3)) and phospholipase C delta pleckstrin homology domain (PLCdelta PH) considering the application to the biotin-avidin techniques. The building blocks of the inositol moiety were synthesized starting with optically resolved myo-inositol derivatives and assembled to the biotin linker through a phosphate linkage.
Anraku
Kensaku
K
Institute of Health Sciences, Kumamoto Health Science University, 325 Izumi-machi, Kumamoto 861-5598, Japan.
Inoue
Teruhiko
T
Sugimoto
Kenji
K
Morii
Takashi
T
Mori
Yasuo
Y
Okamoto
Yoshinari
Y
Otsuka
Masami
M
D001360:Avidin / Q000737:chemistry*; D001710:Biotin / Q000737:chemistry*; D019921:Biotinylation; D015195:Drug Design; D007295:Inositol Phosphates / Q000138:chemical synthesis* / Q000737:chemistry; D015394:Molecular Structure
D007295:Inositol Phosphates; D001360:Avidin; D001710:Biotin