The adipokine SAA3 is induced by interleukin-1beta in mouse adipocytes.
Serum amyloid A (SAA) 3 has been characterized as an inflammatory adipocyte-secreted acute-phase reactant. In the current study, regulation of SAA3 by the proinflammatory and insulin resistance-inducing cytokine interleukin (IL)-1beta was determined in 3T3-L1 and brown adipocytes. Interestingly, SAA3 mRNA and protein synthesis were dramatically increased by IL-1beta in a time-dependent fashion with maximal induction after 24 h. Furthermore, IL-1beta significantly induced SAA3 mRNA expression dose-dependently with maximal 36.4-fold upregulation seen at 2 ng/ml effector. Moreover, IL-1beta-induced SAA3 expression was mediated by nuclear factor-kappaB and janus kinase 2. Taken together, our data show a potent upregulation of SAA3 by IL-1beta.
Sommer
Grit
G
Department of Internal Medicine III, University of Leipzig, 04103 Leipzig, Germany.
Weise
Sebastian
S
Kralisch
Susan
S
Scherer
Philipp E
PE
Lössner
Ulrike
U
Blüher
Matthias
M
Stumvoll
Michael
M
Fasshauer
Mathias
M
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D053583:Interleukin-1beta / Q000494:pharmacology*; D053614:Janus Kinase 2 / Q000378:metabolism; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000685:Serum Amyloid A Protein / Q000235:genetics* / Q000378:metabolism*; D013997:Time Factors
D053583:Interleukin-1beta; D016328:NF-kappa B; D012333:RNA, Messenger; C076498:Saa3 protein, mouse; D000685:Serum Amyloid A Protein; D053614:Janus Kinase 2