Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense.
Detailed structures of the active polysaccharides extracted from the leaf and stem cell walls and mucilage of Dendrobium huoshanense are determined by using various techniques, including chromatographic, spectroscopic, chemical, and enzymatic methods. The mucilage polysaccharide exhibits specific functions in activating murine splenocytes to produce several cytokines including IFN-gamma, IL-10, IL-6, and IL-1alpha, as well as hematopoietic growth factors GM-CSF and G-CSF. However, the deacetylated mucilage obtained from an alkaline treatment fails to induce cytokine production. The structure and bioactivity of mucilage components are validated by further fractionation. This is the first study that provides clear evidence for the structure and activity relationship of the polysaccharide in D. huoshanense.
Hsieh
Yves S-Y
YS
The Genomics Research Center, Academia Sinica, Taipei 115, Taiwan.
Chien
Cheng
C
Liao
Sylvian K-S
SK
Liao
Shih-Fen
SF
Hung
Wei-Ting
WT
Yang
Wen-Bin
WB
Lin
Chih-Chien
CC
Cheng
Ting-Jen Rachel
TJ
Chang
Chia-Chuan
CC
Fang
Jim-Min
JM
Wong
Chi-Huey
CH
D000818:Animals; D001089:Arabinose / Q000032:analysis; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects; D002473:Cell Wall / Q000737:chemistry; D005591:Chemical Fractionation; D016207:Cytokines / Q000096:biosynthesis; D031667:Dendrobium / Q000737:chemistry*; D008516:Medicine, Chinese Traditional; D051379:Mice; D008969:Molecular Sequence Data; D010368:Pectins / Q000032:analysis; D010429:Pentoses / Q000032:analysis; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D013154:Spleen / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D014990:Xylans / Q000032:analysis
D016207:Cytokines; D010429:Pentoses; D011134:Polysaccharides; D014990:Xylans; D010368:Pectins; D001089:Arabinose; C003181:polygalacturonic acid