title: The nucleocapsid protein of severe acute respiratory syndrome coronavirus inhibits cell cytokinesis and proliferation by interacting with translation elongation factor 1alpha.
abstract: Severe acute respiratory syndrome coronavirus (SARS-CoV) is the etiological agent of SARS, an emerging disease characterized by atypical pneumonia. Using a yeast two-hybrid screen with the nucleocapsid (N) protein of SARS-CoV as a bait, the C terminus (amino acids 251 to 422) of the N protein was found to interact with human elongation factor 1-alpha (EF1alpha), an essential component of the translational machinery with an important role in cytokinesis, promoting the bundling of filamentous actin (F-actin). In vitro and in vivo interaction was then confirmed by immuno-coprecipitation, far-Western blotting, and surface plasmon resonance. It was demonstrated that the N protein of SARS-CoV induces aggregation of EF1alpha, inhibiting protein translation and cytokinesis by blocking F-actin bundling. Proliferation of human peripheral blood lymphocytes and other human cell lines was significantly inhibited by the infection of recombinant retrovirus expressing SARS-CoV N protein.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhou
authors.forename: Junli
authors.initials: J
authors.lastname: Liu
authors.forename: Qiuna
authors.initials: Q
authors.lastname: Wang
authors.forename: Xuan
authors.initials: X
authors.lastname: Liu
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Ma
authors.forename: Cheng
authors.initials: C
authors.lastname: Cao
mesh_terms: D000199:Actins / Q000378:metabolism; D026481:Blotting, Far-Western; D002460:Cell Line; D049109:Cell Proliferation; D000086462:Coronavirus Nucleocapsid Proteins; D048749:Cytokinesis / Q000502:physiology*; D006801:Humans; D047468:Immunoprecipitation; D008214:Lymphocytes / Q000821:virology; D019590:Nucleocapsid Proteins / Q000378:metabolism*; D020648:Peptide Elongation Factor 1 / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000502:physiology*; D025941:Protein Interaction Mapping; D045473:Severe acute respiratory syndrome-related coronavirus / Q000502:physiology*; D020349:Surface Plasmon Resonance; D020798:Two-Hybrid System Techniques
chemical_list: D000199:Actins; D000086462:Coronavirus Nucleocapsid Proteins; D019590:Nucleocapsid Proteins; D020648:Peptide Elongation Factor 1