Proteomic response to iron deficiency in tomato root.
To know the root adjustment in response to iron deficiency, differentially displayed proteins in tomato roots of wild type and its iron uptake inefficient mutant T3238fer were analyzed by 2-DE and MALDI-TOF MS-based proteomic method under iron sufficiency and deficiency. Ninety-seven proteins were identified, 63 of them were classified in various metabolic pathways. About 40 proteins involved in starch degradation, TCA and ascorbate cycles were upregulated under iron deficiency and grouped in a network together with glycolysis, whereas proteins for fructose metabolism were decreased. Proteins involved in methionine synthesis, cell wall synthesis, mitochondria ATP synthesis, vacuole ATPase, HSP70/90, etc. also revealed enhanced expression under iron deficiency, while proteins about redox homeostasis, transcription factors, kinases, etc. showed diversified changes. The responses are closely associated with energy metabolism, organic acid formation, root morphological change, redox and sulfur homeostasis, and signal transduction, which enhance iron uptake, reutilization and other adaptive changes. Most of the proteins affected by iron deficiency and fer mutation showed similar effect on individual proteins or pathways, but the independent function of FER to iron deficiency were statistically indicated.
Li
Jie
J
The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Wu
Xu-Dong
XD
Hao
Shan-Ting
ST
Wang
Xiu-Jie
XJ
Ling
Hong-Qing
HQ
D001205:Ascorbic Acid; D001671:Biochemistry / Q000379:methods; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007501:Iron / Q000378:metabolism*; D000090463:Iron Deficiencies; D018551:Solanum lycopersicum / Q000378:metabolism*; D008715:Methionine / Q000737:chemistry; D009154:Mutation; D010084:Oxidation-Reduction; D010940:Plant Proteins / Q000737:chemistry; D018517:Plant Roots; D017510:Protein Folding; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D010940:Plant Proteins; D008715:Methionine; D007501:Iron; D001205:Ascorbic Acid