title: Intrinsic bent DNA sites in the developmentally amplified C3-22 gene promoter of Rhynchosciara americana (Diptera: Sciaridae).
abstract: The Rhynchosciara americana C3-22 gene is located in an amplified domain and is developmentally expressed. The aim of the present work was to identify intrinsically bent DNA sites in a segment containing the gene promoter and downstream sequence. The results indicated that this gene is flanked by intrinsically bent DNA sites. Three bent DNA sites (b(-3), b(-2), and b(-1)) were localized in the promoter, and one was localized downstream of the gene (b(+1)). These sites had helical parameters that confirmed the curved structure, as well as segments with left-handed superhelical writhe. In silico analysis of the promoters of four other insect genes, which encode secreted polypeptides, showed that they all had curved structures and similar helical parameters. Correlation with other results indicates that the detected intrinsically bent DNA sites that flank the C3-22 gene might be a consensus feature of the gene structure in the amplified domains.
authors.affiliation: Departamento de Biologia Celular e Genética, Universidade Estadual de Maringá, UEM-87020-900, Maringá, Paraná, Brazil.
authors.forename: Fabiana de Souza
authors.initials: Fde S
authors.lastname: Gouveia
authors.forename: Fabrícia
authors.initials: F
authors.lastname: Gimenes
authors.forename: Adriana
authors.initials: A
authors.lastname: Fiorini
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: Fernandez
mesh_terms: D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D004175:Diptera / Q000235:genetics*; D004586:Electrophoresis; D005784:Gene Amplification / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D017344:Genes, Insect / Q000235:genetics; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D004247:DNA