[Effect of epidermal growth factor on transcription of MCP-1 in rat dental follicle cells].
OBJECTIVE
To study the effects of epidermal growth factor (EGF) on the secretion of monocyte chemotactic protein-one (MCP-1) in rat dental follicle cells.


METHODS
Dental follicle was harvested from 4-6 days postnatal Wistar rats and cultured in alpha-MEM with 15% FBS. Cells in the third passage were incubated with EGF at different concentrations to choose the best dosage. MTT assays were used to evaluate cell proliferation. Using the suitable concentration, 10 ng/ml EGF cells were cultured for 0, 0.5, 1, 3 and 6 hours respectively, and then collected for RT-PCR analysis. The results were analyzed with SPSS13.0 software package.


RESULTS
EGF(ng/ml) at 5-10 ng/ml increased the cell proliferation (P<0.05), which reached the peak at 10 ng/ml according to MTT assay (P<0.01). 10 ng/ml and 3 hours EGF exposure up-regulated the cellular MCP-1 expression dramatically (P<0.01).


CONCLUSIONS
Proper concentration of EGF may promote dental follicle cell proliferation, EGF may up-regulate MCP-1 transcription in the rat dental follicle cells.
Liu
Zong-Xia
ZX
Department of Periodontology, Stomatological Hospital of Shandong University, Jinan, China.
Wang
Jian
J
Yu
Lan
L
Tang
Kai-Liang
KL
Wang
Xin
X
Li
Shu
S
D000818:Animals; D049109:Cell Proliferation / Q000502:physiology; D002478:Cells, Cultured; D018932:Chemokine CCL2 / Q000096:biosynthesis*; D003795:Dental Sac / Q000502:physiology*; D066255:EGF Family of Proteins; D004815:Epidermal Growth Factor / Q000502:physiology*; D009930:Organic Chemicals; D051381:Rats; D017208:Rats, Wistar
C491766:Ccl2 protein, rat; D018932:Chemokine CCL2; D066255:EGF Family of Proteins; D009930:Organic Chemicals; C420642:alpha minimal essential medium; D004815:Epidermal Growth Factor