title: Inherited human cPLA(2alpha) deficiency is associated with impaired eicosanoid biosynthesis, small intestinal ulceration, and platelet dysfunction.
abstract: Cytosolic phospholipase A2alpha (cPLA2alpha) hydrolyzes arachidonic acid from cellular membrane phospholipids, thereby providing enzymatic substrates for the synthesis of eicosanoids, such as prostaglandins and leukotrienes. Considerable understanding of cPLA2alpha function has been derived from investigations of the enzyme and from cPLA2alpha-null mice, but knowledge of discrete roles for this enzyme in humans is limited. We investigated a patient hypothesized to have an inherited prostanoid biosynthesis deficiency due to his multiple, complicated small intestinal ulcers despite no use of cyclooxygenase inhibitors. Levels of thromboxane B2 and 12-hydroxyeicosatetraenoic acid produced by platelets and leukotriene B4 released from calcium ionophore-activated blood were markedly reduced, indicating defective enzymatic release of the arachidonic acid substrate for the corresponding cyclooxygenase and lipoxygenases. Platelet aggregation and degranulation induced by adenosine diphosphate or collagen were diminished but were normal in response to arachidonic acid. Two heterozygous single base pair mutations and a known SNP were found in the coding regions of the patient's cPLA2alpha genes (p.[Ser111Pro]+[Arg485His; Lys651Arg]). The total PLA2 activity in sonicated platelets was diminished, and the urinary metabolites of prostacyclin, prostaglandin E2, prostaglandin D2, and thromboxane A2 were also reduced. These findings characterize what we believe is a novel inherited deficiency of cPLA2.
authors.affiliation: Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, Nashville, Tennessee 37232-6602, USA.
authors.forename: David H
authors.initials: DH
authors.lastname: Adler
authors.forename: Joy D
authors.initials: JD
authors.lastname: Cogan
authors.forename: John A
authors.initials: JA
authors.lastname: Phillips
authors.forename: Nathalie
authors.initials: N
authors.lastname: Schnetz-Boutaud
authors.forename: Ginger L
authors.initials: GL
authors.lastname: Milne
authors.forename: Tina
authors.initials: T
authors.lastname: Iverson
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Stein
authors.forename: David A
authors.initials: DA
authors.lastname: Brenner
authors.forename: Jason D
authors.initials: JD
authors.lastname: Morrow
authors.forename: Olivier
authors.initials: O
authors.lastname: Boutaud
authors.forename: John A
authors.initials: JA
authors.lastname: Oates
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D001792:Blood Platelets / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D015777:Eicosanoids / Q000378:metabolism*; D054513:Group IV Phospholipases A2 / Q000172:deficiency* / Q000235:genetics* / Q000502:physiology; D006579:Heterozygote; D006801:Humans; D007421:Intestine, Small / Q000473:pathology*; D007476:Ionophores / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D008954:Models, Biological; D015230:Prostaglandin D2 / Q000378:metabolism; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism; D013928:Thromboxane A2 / Q000378:metabolism; D014456:Ulcer / Q000473:pathology
chemical_list: D015777:Eicosanoids; D007476:Ionophores; D016718:Arachidonic Acid; D013928:Thromboxane A2; D011451:Prostaglandin-Endoperoxide Synthases; D054513:Group IV Phospholipases A2; D015230:Prostaglandin D2; D002118:Calcium