title: An increase in kisspeptin-54 release occurs with the pubertal increase in luteinizing hormone-releasing hormone-1 release in the stalk-median eminence of female rhesus monkeys in vivo.
abstract: The G-protein coupled receptor GPR54 and its ligand, KiSS-1-derived peptide kisspeptin-54, appear to play an important role in the mechanism of puberty. This study measures the release of kisspeptin-54 in the stalk-median eminence (S-ME) during puberty and examines its potential role in the pubertal increase in LHRH-1 release in female rhesus monkeys. First, developmental changes in release of kisspeptin-54 and LHRH-1 were assessed in push-pull perfusate samples obtained from the S-ME of prepubertal, early pubertal, and midpubertal female rhesus monkeys. Whereas LHRH-1 levels in 10-min intervals had been measured previously for other experiments, kisspeptin-54 levels in 40-min pooled samples were newly measured by RIA. The results indicate that a significant increase in kisspeptin-54 release occurred in association with the pubertal increase in LHRH-1 release and that a nocturnal increase in kisspeptin-54 release was already observed in prepubertal monkeys and continued through the pubertal period. Second, we measured kisspeptin-54 release in the S-ME of midpubertal monkeys at 10-min intervals using a microdialysis method. Kisspeptin-54 release in the S-ME was clearly pulsatile with an interpulse interval of about 60 min, and approximately 75% of kisspeptin-54 pulses were correlated with LHRH-1 pulses. Finally, the effect of kisspeptin-10 on LHRH-1 release was examined with the microdialysis method. Kisspeptin-10 infusion through a microdialysis probe significantly stimulated LHRH-1 release in a dose-dependent manner. Collectively, the results are consistent with the hypothesis that kisspeptin plays a role in puberty.
authors.affiliation: Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin 53715-1299, USA.
authors.forename: Kim L
authors.initials: KL
authors.lastname: Keen
authors.forename: Frederick H
authors.initials: FH
authors.lastname: Wegner
authors.forename: Stephen R
authors.initials: SR
authors.lastname: Bloom
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Ghatei
authors.forename: Ei
authors.initials: E
authors.lastname: Terasawa
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism*; D059648:Kisspeptins; D008253:Macaca mulatta / Q000378:metabolism* / Q000502:physiology; D008473:Median Eminence / Q000378:metabolism*; D017551:Microdialysis; D009842:Oligopeptides / Q000008:administration & dosage / Q000494:pharmacology; D011673:Pulsatile Flow / Q000187:drug effects; D012741:Sexual Maturation / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D025521:Tumor Suppressor Proteins / Q000378:metabolism* / Q000502:physiology
chemical_list: C106628:KISS1 protein, human; D059648:Kisspeptins; D009842:Oligopeptides; D025521:Tumor Suppressor Proteins; D007987:Gonadotropin-Releasing Hormone