Moving EM: the Rapid Transfer System as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing.
In this paper, the Rapid Transfer System (RTS), an attachment to the Leica EMPACT2 high-pressure freezer, is described as a new tool for special applications within the cryofixation field. The RTS is an automated system that allows for fast processing of samples (<5 s) that make it possible for the first time to use high-pressure freezing in combination with high time resolution correlative light and electron microscopy. In addition, with a working cycle of 30 s this rapid turn over time allows one to acquire more samples of biopsy material before it deteriorates than with other HPF machines with longer cycle times. With the use of the RTS it was possible to obtain three samples each of four different tissues in 6 min. Together with the finding that 90% of samples of cells grown on sapphire discs were well frozen, the RTS was both fast and reliable. Most important, together with other newly developed accessories, the RTS made it possible to capture specific events occurring live in the cell as observed by light microscopy, to cryofix that sample/event within 4 s, and then to analyze that event at high resolution in the electron microscope with excellent preservation of ultra-structure. These developments should give us the tools to unravel intracellular processes that can be observed by live cell imaging but are too rare or fast to be picked up by routine EM methods or where the history of a structure is necessary to be able to discern its nature.
Verkade
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School of Medical Sciences, University Walk, Bristol, BS8 1TD, United Kingdom. p.verkade@bristol.ac.uk
D000818:Animals; D001706:Biopsy; D002460:Cell Line; D015925:Cryopreservation / Q000379:methods; D005615:Freezing; D007515:Islets of Langerhans / Q000166:cytology / Q000648:ultrastructure; D051379:Mice; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D008854:Microscopy, Electron / Q000295:instrumentation* / Q000379:methods; D010179:Pancreas / Q000166:cytology; D011312:Pressure; D013997:Time Factors