title: [Culture of rabbit parotid acinar cells and evaluation of its excretion function in vitro].
abstract: OBJECTIVE
  To obtain a large number of rabbit parotid acinar cells with excretion function in vitro.
  
  
  METHODS
  Rabbit parotid acinar cells were cultured in the reformed DMEM; The shape and structure of cultured cells were observed with phase contrast microscope; Pan cytokeratin(PCK), alpha-amylase and actin of the cultured parotid acinar cells were examined with immunocytochemical SP technique; the secretion of different generations was evaluated with positive expression status of alpha-amylase. SPSS10.0 software package for windows was used for Student's t test.
  
  
  RESULTS
  The acinar cells had the ability of proliferation, they could pass 4 generations and survive for 4 weeks. The specific immunocytochemical staining for PCK and alpha-amylase could be seen in the plasm and (or) membrane of parotid acinar cells, but not for actin. The positive expression of alpha-amylase in the primary generation, generation 1, 2 and 3 was 79.6%, 76%, 47.7% and 2.4%, respectively, which decreased gradually. There was a significant difference between the second generation and two earlier generations(P<0.05).
  
  
  CONCLUSIONS
  The primary and first generations of parotid acinar cells maintain their characteristics and proliferation in vitro,which is very useful for further experiment.
authors.affiliation: Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China. hewei1968@tom.com
authors.forename: Wei
authors.initials: W
authors.lastname: He
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Qiao
authors.forename: Long-Jiang
authors.initials: LJ
authors.lastname: Li
mesh_terms: D061354:Acinar Cells; D000818:Animals; D002478:Cells, Cultured; D066298:In Vitro Techniques; D010306:Parotid Gland / Q000166:cytology*; D012333:RNA, Messenger; D011817:Rabbits; D000516:alpha-Amylases / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D000516:alpha-Amylases