Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples.
The NucliSens easyMAG and BioRot 9604 automated nucleic acid extraction systems were evaluated and compared with the manual QIAamp (Qiagen) extraction method for their abilities to extract nucleic acid from nasopharyngeal aspirate samples for the detection of RNA and DNA respiratory viruses. The nucleic acids recovered by all three methods gave comparable sensitivities in PCR tests, and the three methods gave comparable viral loads. There was no evidence of residual PCR inhibitors and no evidence of PCR cross-contamination.
Chan
Kwok Hung
KH
Department of Microbiology, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong, SAR, People's Republic of China.
Yam
Wing Cheong
WC
Pang
Chiu Mei
CM
Chan
Kit Man
KM
Lam
Siu Yan
SY
Lo
Kam Fai
KF
Poon
Leo L M
LL
Peiris
J S Malik
JS
D001331:Automation; D004267:DNA Viruses / Q000302:isolation & purification*; D004279:DNA, Viral / Q000302:isolation & purification*; D006723:Hong Kong; D006761:Hospitals; D006801:Humans; D009305:Nasopharynx / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012328:RNA Viruses / Q000302:isolation & purification*; D012367:RNA, Viral / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D014773:Virology / Q000379:methods*; D014777:Virus Diseases / Q000175:diagnosis*
D004279:DNA, Viral; D012367:RNA, Viral