Time course of infection with Salmonella typhimurium and its influence on fecal shedding, distribution in inner organs, and antibody response in fattening pigs.
This is the first longitudinal study conducted over the entire 5-month fattening period in pigs to investigate the infection dynamics of Salmonella Typhimurium and the association between antibody response and the prevalence of these bacteria in feces. A total of 16 weaning pigs were infected with Salmonella Typhimurium DT104 followed by clinical examination and blood and fecal sampling until slaughter 138 days postinoculation. To investigate fecal shedding rates and distribution patterns of Salmonella in internal organs regarding premortem stress, one group of swine was transported before slaughter; the other group was slaughtered without being transported. A positive correlation between bacteremia-associated fever and fecal shedding rate was observed, although 69% (11 of 16) of infected pigs had no diarrhea. All animals excreted Salmonella Typhimurium at high levels within 2 weeks postinoculation; thereafter, the number of positive pigs declined and Salmonella shedding became intermittent. In contrast, the proportion of pigs that tested seropositive was higher over the entire fattening period (except during the first 3 weeks postinoculation), revealing the advantage of enzyme-linked immunosorbent assay for Salmonella screening on herd level. Concerning the distribution in internal organs and cross-contamination during slaughter, the highest level of Salmonella was detected in tonsils and jejunal and ileocecal lymph nodes, whereas salmonellae could not be detected in muscle, spleen, and liver. No specific influence of transport-induced stress on Salmonella shedding rates in feces and distribution patterns in organs was observed.
Scherer
Kathrin
K
Federal Institute for Risk Assessment, Division of Biological Safety, Diedersdorfer Weg 1, 12277 Berlin, Germany. kathrin.scherer@bfr.bund.de
Szabó
István
I
Rösler
Uwe
U
Appel
Bernd
B
Hensel
Andreas
A
Nöckler
Karsten
K
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D015169:Colony Count, Microbial; D003257:Consumer Product Safety; D005243:Feces / Q000382:microbiology*; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control; D006801:Humans; D009928:Organ Specificity; D012481:Salmonella Infections, Animal / Q000175:diagnosis* / Q000382:microbiology / Q000635:transmission; D012486:Salmonella typhimurium / Q000276:immunology* / Q000302:isolation & purification; D016036:Seroepidemiologic Studies; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000382:microbiology / Q000635:transmission; D014186:Transportation
D000907:Antibodies, Bacterial