[Preparation and osteoinduction capacity of collagen/bone morphogenetic protein composites].
Collagen/bone morphogenetic protein composites were prepared with collagen type I sponge and bone morphogenetic protein-2 (BMP-2). The composites were implanted into Latissimus dorsi muscles pouches of rabbits. Samples were studied with ALP staining, Von Kossa staining, HE staining, toluidine blue staining and CD31 histochemical labeling of microvessel. Bony samples were then used to repair mandibular defect. The effects were evaluated by X-ray, compressive strength, economycin fluorescence labeling, HE staining, toluidine blue staining and bone quantity analysis. Bone formation induced by collagen/BMP composites was found as woven bone between 4 and 6 weeks; cartilaginous osteogenesis was the main type of bone formation; microvessels could be seen in the bony tissues; and the bone defects were healed completely 6 weeks after operation. Bone formation induced by collagen/BMP composites in the muscles can be used as a donor to repair the bone defect.
Tan
Weiqiang
W
Department of Plastic Surgery, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. tanweixxxx@163.com
Xu
Jinghong
J
Liu
Youshan
Y
Lin
Jun
J
Wang
Huiming
H
D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000737:chemistry / Q000627:therapeutic use*; D001861:Bone Regeneration / Q000187:drug effects; D016025:Bone Transplantation / Q000379:methods*; D024042:Collagen Type I / Q000737:chemistry / Q000627:therapeutic use*; D005260:Female; D020007:Implants, Experimental; D008297:Male; D008338:Mandibular Injuries / Q000601:surgery*; D018482:Muscle, Skeletal / Q000601:surgery; D011817:Rabbits; D011897:Random Allocation
D055396:Bone Morphogenetic Protein 2; D024042:Collagen Type I