title: Neonates with culture proven sepsis have lower amounts and percentage of CD45RA+ T cells.
abstract: To evaluate the expression of lymphocyte subsets in newborns diagnosed as culture proven or culture negative sepsis and to investigate the differentiation. The aim of this study is to explore neonatal immunology in newborns diagnosed as culture proven or culture negative neonatal sepsis and to identify their place in the diagnosis. This prospective study was performed in newborns who were diagnosed as neonatal sepsis and hospitalized in a tertiary care hospital and who were classified as culture proven sepsis (n=12), as culture negative sepsis (n=21) and healthy (n=17). Lymphocyte subsets were obtained at time of diagnosis. Culture proven sepsis had statistically significant increase of WBC compared to culture negative sepsis and control groups (p<0.05). Significant decreases were observed of percentage of lymphocyte, when compared to culture negative sepsis and control group (p<0.05). Percentage of CD4(+) was lower in culture proven sepsis and absolute count of CD4(+) was lower in culture negative sepsis (p<0.05). Percentage and absolute count of CD45RA(+) were lower in culture negative sepsis than control and percentage of CD45RA(+) was lower in culture proven sepsis than control (p<0.05). Percentage of CD45RO(+) was higher in culture proven sepsis than control group (p<0.05). It is clear that during neonatal sepsis lymphocyte subsets are different from healthy controls. Whether the described abnormalities represent the absence of a normal maturation process, rather, pathological events is still not clear.
authors.affiliation: Department of Pediatrics, Medical Faculty of Firat University, 23119, Elazig, Turkey.
authors.forename: A Denizmen
authors.initials: AD
authors.lastname: Aygun
authors.forename: A Nese
authors.initials: AN
authors.lastname: Citak Kurt
authors.forename: Ahmet
authors.initials: A
authors.lastname: Godekmerdan
authors.forename: Abdullah
authors.initials: A
authors.lastname: Kurt
authors.forename: Saadet
authors.initials: S
authors.lastname: Akarsu
authors.forename: M Kaya
authors.initials: MK
authors.lastname: Gurgoze
authors.forename: Erdal
authors.initials: E
authors.lastname: Yilmaz
mesh_terms: D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D017493:Leukocyte Common Antigens / Q000378:metabolism*; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D008297:Male; D011446:Prospective Studies; D018805:Sepsis / Q000175:diagnosis / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000473:pathology
chemical_list: D017493:Leukocyte Common Antigens