Disruption of metF increased L-lysine production by Methylophilus methylotrophus from methanol.
Methionine auxotrophic mutants of Methylophilus methylotrophus AS1 expressing a mutant form of dapA (dapA24) encoding a dihydrodipicolinate synthase desensitized from feedback inhibition by L-lysine, and mutated lysE (lysE24) encoding the L-lysine exporter from Corynebacterium glutamicum 2256, produced higher amounts of L-lysine from methanol as sole carbon source than did other amino acid auxotrophic mutants. Especially, the M. methylotrophus 102 strain, carrying both dapA24 and lysE24, produced L-lysine in more than 1.5 times amounts higher than the parent. A single-base substitution was identified in this auxotroph in codon-329 of the open reading frame of metF, encoding 5,10-methylene-tetra-hydrofolate reductase. We constructed a metF disruptant mutant carrying both dapA24 and lysE24, and confirmed increases in L-lysine production. This is the first report to the effect that metF deficient increased L-lysine production in methylotroph.
Ishikawa
Kohei
K
Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc., Kawasaki 210-8681, Japan. kouhei_ishikawa@ajinomoto.com
Asahara
Takayuki
T
Gunji
Yoshiya
Y
Yasueda
Hisashi
H
Asano
Kozo
K
D045103:5,10-Methylenetetrahydrofolate Reductase (FADH2) / Q000172:deficiency / Q000235:genetics* / Q000378:metabolism*; D008239:Lysine / Q000096:biosynthesis*; D000432:Methanol / Q000378:metabolism*; D008715:Methionine / Q000378:metabolism; D020630:Methylophilus methylotrophus / Q000201:enzymology / Q000378:metabolism*; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism
D008715:Methionine; D045103:5,10-Methylenetetrahydrofolate Reductase (FADH2); D017853:Phosphotransferases (Alcohol Group Acceptor); C021370:homoserine kinase; D008239:Lysine; D000432:Methanol