[Diagnostic role of different immunologic methods for laboratory diagnostics of legionellosis].
The aim of the study was a comparative analysis of diagnostic value of different laboratoty methods conducted on the basis of results of examination of patients during Legionnaires' disease outbreak in town Verkhnyaya Pyshma. Retrospective analysis of laboratory data from 74 patients with diagnosis of Legionnaires' disease was performed. Complex of laboratory methods was used (polymerase chain reaction (PCR), enzyme immunoassay (EIA), immunochromatography). In group of patients with Legionnaires' disease, the highest proportion of positive results (73%) was obtained by the EIA determining total specific antibodies in urine. Determination of antigen in urine by immunochromatographic express-test yielded 52% of positive results. PCR testing of blood specimens yielded positive results in 65% of samples but was low specific, due to that in 19% of patients from control group false-positive results were obtained. Testing of 3 autopsy samples showed that all specimens contained DNA of the causative agent. Performed analysis allowed to recommend complex use of immunochromatographic express-test of antigen detection and identification of total specific antibodies by EIA during mass people examination.
Mikhaĭlova
D O
DO
Bobyleva
Z D
ZD
Bazarnyi
V V
VV
Amon
E P
EP
Beĭkin
Ia B
IaB
Besedina
L G
LG
Mel'nikova
O V
OV
Shilova
V P
VP
Rozanova
S M
SM
Perevalova
E Iu
EIu
D000907:Antibodies, Bacterial / Q000097:blood / Q000652:urine; D000942:Antigens, Bacterial / Q000652:urine; D002846:Chromatography, Affinity; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D005189:False Positive Reactions; D006801:Humans; D007124:Immunoenzyme Techniques; D007875:Legionella / Q000235:genetics / Q000276:immunology; D007876:Legionellosis / Q000097:blood / Q000175:diagnosis* / Q000652:urine; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012426:Russia; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial