Hyperbaric oxygen therapy effects on tissue lesions in acute pancreatitis. Experimental study in rats.
OBJECTIVE
To study the effects of hyperbaric oxygen therapy on tissue lesions in an experimental model of acute pancreatitis induced by pancreatic duct ligation.


METHODS
Forty-eight adult female Wistar rats were randomized into two groups (n=24): control group and hyperbaric oxygen therapy group.


METHODS
The second group was treated with a two-hour daily session of hyperbaric oxygen therapy at 2.5 ATA started 6 hours after pancreatic duct ligation.


METHODS
The two groups were divided into 3 subgroups of 8 rats each undergoing euthanasia on days 1, 3, and 7 after the acute pancreatitis induction.


METHODS
The pancreas was evaluated according to the following histopathologic criteria: edema, hemorrhage, acinar necrosis and leukocyte infiltration.


RESULTS
Hyperbaric oxygen therapy was efficient in significantly reducing acinar necrosis on the first day (P=0.049) and the foci of hemorrhage on the seventh day (P=0.050). The edema and leukocyte infiltration did not show the expected reduction.


CONCLUSIONS
The utilization of a daily session of hyperbaric oxygen therapy at 2.5 ATA is efficient in reducing the hemorrhage and acinar necrosis but is not sufficient to reduce edema and leukocyte infiltration.
Festugato
Marcio
M
Laboratory of Physiology, Center of Biological Sciences, University of Caxias do Sul (UCS), Caxias do Sul, RS, Brazil.
Coelho
Celso Picolli
CP
Fiedler
Gustavo
G
Machado
Felipe Pioner
FP
Gonçalves
Mauro Cabral
MC
Bassani
Filipe Rech
FR
Pierezan
Paulo Henrique
PH
Osvaldt
Alessandro Bersch
AB
Rohde
Luiz
L
D000208:Acute Disease; D000681:Amylases / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D006470:Hemorrhage / Q000175:diagnosis; D006931:Hyperbaric Oxygenation; D009336:Necrosis / Q000175:diagnosis; D010195:Pancreatitis / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar; D012107:Research Design
D000681:Amylases