title: Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.
abstract: Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.
authors.affiliation: Institute for Molecular Biophysics, The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.
authors.forename: Manuel F
authors.initials: MF
authors.lastname: Juette
authors.forename: Travis J
authors.initials: TJ
authors.lastname: Gould
authors.forename: Mark D
authors.initials: MD
authors.lastname: Lessard
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Mlodzianoski
authors.forename: Bhupendra S
authors.initials: BS
authors.lastname: Nagpure
authors.forename: Brian T
authors.initials: BT
authors.lastname: Bennett
authors.forename: Samuel T
authors.initials: ST
authors.lastname: Hess
authors.forename: Joerg
authors.initials: J
authors.lastname: Bewersdorf
mesh_terms: D001703:Biophysics / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D019793:Fluorescein / Q000494:pharmacology; D005456:Fluorescent Dyes / Q000494:pharmacology; D007089:Image Enhancement; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D007834:Lasers; D008027:Light; D008853:Microscopy / Q000379:methods; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D012984:Software
chemical_list: D005456:Fluorescent Dyes; D019793:Fluorescein