Periostin and bone marrow fibrosis.
Periostin is a secreted protein that shares structural homology with the insect axon guidance protein fasciclin 1. Periostin is expressed predominantly in collagen-rich fibrous connective tissues that are subjected to constant mechanical stresses. We have shown previously that periostin is a novel component of subepithelial fibrosis in bronchial asthma. Here, we investigated the relationship between periostin and bone marrow (BM) fibrosis. Periostin was expressed in the stroma and stromal cells of BM fibrosis specimens and to a great extent its expression levels correlated closely to the grade of fibrosis, as estimated by silver staining. However, in the present study, we found no relationship between plasma periostin levels and the extent of BM fibrosis. We also demonstrated that periostin is secreted by human BM hTERT stromal cells and that its secretion is enhanced by TGF-beta, a cytokine produced by clonal proliferation of megakaryocytes and/or monocytes. These results indicate that periostin is a component of BM fibrosis and that it may play a role in the disease progression.
Oku
Eijiro
E
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Kanaji
Taisuke
T
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan. kanajit@med.kurume-u.ac.jp.
Takata
Yuka
Y
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Oshima
Koichi
K
Department of Pathology, Kurume University School of Medicine, Kurume, Japan.
Seki
Ritsuko
R
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Morishige
Satoshi
S
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Imamura
Rie
R
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Ohtsubo
Korenori
K
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Hashiguchi
Michitoshi
M
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Osaki
Koichi
K
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Yakushiji
Kazuaki
K
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Yoshimoto
Kohji
K
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Ogata
Hideaki
H
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
Hamada
Hirofumi
H
Department of Molecular Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.
Izuhara
Kenji
K
Division of Medical Biochemistry, Department of Biomolecular Sciences, Saga Medical School, Saga, Japan.
Sata
Michio
M
Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, Kurume, Japan.
Okamura
Takashi
T
Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
D000368:Aged; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism / Q000473:pathology; D055728:Primary Myelofibrosis / Q000378:metabolism* / Q000473:pathology; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis
D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D016212:Transforming Growth Factor beta