Molecular characterization of Enterococcus faecalis N06-0364 with low-level vancomycin resistance harboring a novel D-Ala-D-Ser gene cluster, vanL.
Enterococcus faecalis N06-0364, exhibiting a vancomycin MIC of 8 microg/ml, was found to harbor a novel D-Ala-D-Ser gene cluster, designated vanL. The vanL gene cluster was similar in organization to the vanC operon, but the VanT serine racemase was encoded by two separate genes, vanTm(L) (membrane binding) and vanTr(L) (racemase).
Boyd
David A
DA
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Willey
Barbara M
BM
Fawcett
Darlene
D
Gillani
Nazira
N
Mulvey
Michael R
MR
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D010453:Peptide Synthases / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D020713:Vancomycin Resistance / Q000235:genetics
D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D010453:Peptide Synthases; C108395:VanC protein, Enterococcus