Determination of binding-dioxygen in dioxygen complexes by headspace gas chromatography.
Dioxygen complexes play important roles in organisms' bodies, so the determination of binding-dioxygen has practical significance. A simple and robust method based on headspace gas chromatography was proposed to determine the binding-dioxygen in dioxygen complexes. By measuring the content change of nitrogen gas in a vial, the amount of oxygen released from dixoygen complexes can be determined. The method was validated using potassium chlorate as model sample, and the results exhibited good recoveries (90-99%) with the relative standard deviation less than 8%. It was also used to analyze dioxygen complex of cobalt bis(salicylaldehyde) ethylenediimine and polyamine cobalt complexes prepared by solid-phase reaction.
Wang
Wei
W
Key Laboratory of Oil & Gas Fine Chemicals, Ministry of Education, Urumqi 830046, China.
Feng
Shun
S
Li
Ya-ni
YN
Wu
Meiying
M
Wang
Jide
J
D002849:Chromatography, Gas / Q000379:methods*; D010100:Oxygen / Q000737:chemistry*; D012680:Sensitivity and Specificity
D010100:Oxygen