Parallel confocal detection of single molecules in real time.
The confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations. This is achieved through the use of a holographic laser illumination multiplexer combined with a confocal pinhole array before a prism dispersive element used to provide spectroscopic information from each confocal volume. The system is demonstrated to detect and identify single fluorescent molecules from each of several thousand independent confocal volumes in real time.
Lundquist
Paul M
PM
Pacific Biosciences Inc., 1505 Adams Drive, Menlo Park, California 94025, USA.
Zhong
Cheng F
CF
Zhao
Peiqian
P
Tomaney
Austin B
AB
Peluso
Paul S
PS
Dixon
John
J
Bettman
Brad
B
Lacroix
Yves
Y
Kwo
Deborah P
DP
McCullough
Etienne
E
Maxham
Mark
M
Hester
Kevin
K
McNitt
Paul
P
Grey
Donald M
DM
Henriquez
Carlos
C
Foquet
Mathieu
M
Turner
Stephen W
SW
Zaccarin
Denis
D
D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology
D005456:Fluorescent Dyes