title: Reduction of CD55 and/or CD59 in red blood cells of patients with HIV infection.
abstract: BACKGROUND
  Anemia is a common feature in HIV infection. An increased sensitivity of lymphocytes from HIV patients to lysis by complement has been correlated with a decreased expression of CD55 and CD59 in their surface. The aim of this study was to evaluate CD55/CD59 presence in red cells of HIV patients and explore possible correlations with clinical parameters.
  
  
  METHODS
  CD55/CD59 expression was evaluated in erythrocytes of 37 patients (30M/7F, median age: 39 years) with HIV infection (25 also having hemophilia), 121 controls, and 8 PNH patients using the sephacryl-gel microtyping system. Ham and sucrose tests were also performed.
  
  
  RESULTS
  Anemia was present in 14/37 (37%) HIV patients. Interestingly, all HIV patients had deficient CD55 and/or CD59 erythrocytes: 8 (21%) for both CD55 andCD59 and 29 (78%) isolated CD55 and/or CD59 negativity. Deficient erythrocytes did not account for more than 10% of the total in the vast majority of patients. In controls, only 2 (1%) had red cells with double CD55/CD59 negativity and 3 (2%) had isolated deficiency. All PNH patients had a simultaneous CD55/CD59 deficiency. Positive Ham and sucrose tests were found only in PNH. There was no correlation between the presence of deficient CD55/CD59 erythrocytes and anemia, hemolysis, antiretroviral therapy, CD4+ counts, viral load, or concomitant hepatitis C infection in HIV patients.
  
  
  CONCLUSIONS
  This study provides evidence supporting the presence of erythrocytes with CD55 and/or CD59 deficiency in HIV. Further studies using molecular techniques will be required to clarify the exact role of this deficiency in HIV patients.
authors.affiliation: Department of Medical Research, 251 General Air Force Hospital, Athens, Greece.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Terpos
authors.forename: Angelos
authors.initials: A
authors.lastname: Sarantopoulos
authors.forename: Anna
authors.initials: A
authors.lastname: Kouramba
authors.forename: Olga
authors.initials: O
authors.lastname: Katsarou
authors.forename: John
authors.initials: J
authors.lastname: Stavropoulos
authors.forename: Stavroula
authors.initials: S
authors.lastname: Masouridi
authors.forename: Anastasia
authors.initials: A
authors.lastname: Karafoulidou
authors.forename: John
authors.initials: J
authors.lastname: Meletis
mesh_terms: D000328:Adult; D018958:CD55 Antigens / Q000097:blood*; D018957:CD59 Antigens / Q000097:blood*; D003165:Complement System Proteins; D004912:Erythrocytes / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D015658:HIV Infections / Q000097:blood*; D006457:Hemoglobinuria, Paroxysmal / Q000150:complications; D006467:Hemophilia A / Q000150:complications; D006526:Hepatitis C / Q000150:complications; D006801:Humans; D008297:Male; D008875:Middle Aged; D010641:Phenotype
chemical_list: D018958:CD55 Antigens; D018957:CD59 Antigens; D003165:Complement System Proteins