Gene delivery by adenoviruses.
Adenoviruses have a number advantages as gene delivery vectors, including ability to transduce a wide variety of non-dividing and dividing cells with high efficiency, relative ease of construction, and ability to be purified as high-titer viral stocks. These characteristics make adenoviruses particularly attractive for over-expressing specific genes in vitro and for evaluating in vivo biological activity in animal models. In this chapter, procedures will be described for adenovirus construction and virus delivery to in vivo sites for measurement of osteogenic activity.
Franceschi
Renny T
RT
Department of Periodontics & Oral Medicine, School of Dentistry, and Biological Chemistry, School of Medicine, University of Michigan, Ann Arbor, MI, USA.
Ge
Chunxi
C
D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D019485:Bone Morphogenetic Proteins / Q000235:genetics / Q000378:metabolism; D002460:Cell Line; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D006801:Humans
D019485:Bone Morphogenetic Proteins