Surface charge and zeta-potential of metabolically active and dead cyanobacteria.
Zeta potential and acid-base titrations of active, inactivated, and dead Planktothrix sp. and Synechococcus sp. cyanobacteria were performed to determine the degree to which cell surface electric potential and proton/hydroxyl adsorption are controlled by metabolism or cell membrane structure. Surface OH(-) excess from potentiometric data, showed differences in surface charge between active and dead cyanobacteria from pH 3 to 10. Average zero salt effect pH (pH(pzse)) of 5.8+/-0.1 and 6.3+/-0.1 were obtained for active Planktothrix sp. and Synechococcus sp., respectively. Similarly for dead cyanobacteria pH(pzse) values of 5.8+/-0.1 and 4.6+/-0.1 were obtained. Zeta potentials of active Planktothrix sp. and Synechococcus sp. were positive at alkaline conditions, with a maximum of +13.7+/-1.5 mV at a pH of 9.0+/-0.1 for both species. This positive potential diminished in the presence of 1 mM HCO(-)(3). The zeta potential of Planktothrix sp. and Synechococcus sp. cells was negative at alkaline pH following their exposure to NaN(3), a metabolic inhibitor. The zeta potential of dead cyanobacteria was negative for Planktothrix sp., from pH 2.5 to 10.5, at -30 to -20 mV. Dead Synechococcus sp. exposed to a pH 2.5 solution recorded negative potentials to a minimum of -30 mV at pH 8, but positive potentials were found at higher pH reaching a maximum of +10 mV at pH 9.1. Zeta potentials for dead, but non-acidified Synechococcus sp. remained negative at -30 mV from an initial pH of 5.6 to 10.5, reflecting differences in cell wall structure between these species. These results indicate that Planktothrix sp. and Synechococcus sp. may metabolically control their surface charge to electrostatically attract bicarbonate anions at alkaline pH, required for photosynthesis.
Martinez
Raul E
RE
Laboratoire des Mécanismes et Transferts en Géologie, UMR 5563 CNRS, Université Paul-Sabatier, Observatoire Midi-Pyrénées, 14, avenue Edouard Belin, 31400 Toulouse, France. rmartinez@ipgp.jussieu.fr
Pokrovsky
Oleg S
OS
Schott
Jacques
J
Oelkers
Eric H
EH
D000327:Adsorption; D001703:Biophysics / Q000379:methods*; D002462:Cell Membrane / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism; D000458:Cyanobacteria / Q000378:metabolism*; D004563:Electrochemistry / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008565:Membrane Proteins / Q000737:chemistry; D008954:Models, Biological; D010788:Photosynthesis; D011522:Protons; D055672:Static Electricity; D013499:Surface Properties; D046940:Synechococcus / Q000378:metabolism*
D008565:Membrane Proteins; D011522:Protons