title: Determination of some phenolic acids in Majorana hortensis by capillary electrophoresis with online electrokinetic preconcentration.
abstract: An online accumulation/mobilization preconcentration technique based on a dynamic pH junction technique and electrokinetic injection was employed for analysis of phenolic acids (sinapic, ferulic, coumarinic, caffeic, syringic, vanillic, and 4-hydroxybenzoic acid) in extracts from Majorana hortensis leaves. Samples were extracted by pressurized solvent extraction with acetone at 150 degrees C and 15 MPa. The capillary electrophoretic method employed 50 mmol.L (-1) sodium borate, pH 9.5, as the sample electrolyte, 50 mmol.L (-1) sodium phosphate, pH 2.5, as the background electrolyte, and 50 mmol.L (-1) sodium phosphate, pH 2.5, with 60 mmol.L (-1) sodium dodecyl sulfate as the mobilization electrolyte. The method allowed 720-fold to 5560-fold preconcentration of the phenolic acids during 30 min of electrokinetic accumulation with detection limits from 0.38 to 4.22 ng.mL (-1).
authors.affiliation: Department of Analytical Chemistry, Faculty of Science, Palacky University in Olomouc, Trida Svobody 8, Olomouc, Czech Republic. petrjan1@gmail.com
authors.forename: Jan
authors.initials: J
authors.lastname: Petr
authors.forename: Katerina
authors.initials: K
authors.lastname: Vítková
authors.forename: Václav
authors.initials: V
authors.lastname: Ranc
authors.forename: Joanna
authors.initials: J
authors.lastname: Znaleziona
authors.forename: Vítezslav
authors.initials: V
authors.lastname: Maier
authors.forename: Radim
authors.initials: R
authors.lastname: Knob
authors.forename: Juraj
authors.initials: J
authors.lastname: Sevcík
mesh_terms: D001881:Borates; D003373:Coumaric Acids / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000032:analysis*; D031341:Origanum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*
chemical_list: D001881:Borates; D003373:Coumaric Acids; D062385:Hydroxybenzoates; D010936:Plant Extracts; C010634:sodium borate