title: Isolation of coagulase-negative staphylococci from extended-wear soft contact lenses in asymptomatic patients.
abstract: BACKGROUND
  Coagulase-negative staphylococci and diphtheroids are normal inhabitants of the outer surface of the human eye. These microorganisms serve as part of the defense mechanism of the ocular anatomy in preventing colonization and infection by pathogenic bacteria. Nevertheless, infections associated with contaminated solutions and cases became serious problems for people who wear soft contact lenses. The aim of this study is to isolate and identify aerobic bacteria, particularly, gram-negative species associated with the use of extended-wear soft contact lenses.
  
  
  METHODS
  Extended-wear contact lenses were collected, using aseptic technique, from the eyes of individuals after 30 days of extended wear (5-7 day intermittent periods) and were examined for adhered aerobic bacteria.
  
  
  RESULTS
  Coagulase-negative staphylococci were isolated from 74% of the lenses. Serratia marcescens was found at an incidence of 10% and Pseudomonas aeruginosa at an incidence of 6%.
  
  
  CONCLUSIONS
  The presence of species of bacteria, including P. aeruginosa and S. marcescens, which have been associated with daily wear soft contact lenses, solutions, and cases also seem to be associated with extended-wear lenses.
authors.affiliation: Department of Microbiology, Isfahan University of Medical Sciences, Isfahan, Iran. faghri@med.mui.ac.ir
authors.forename: Jamshid
authors.initials: J
authors.lastname: Faghri
mesh_terms: D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D003262:Contact Lenses, Extended-Wear / Q000382:microbiology*; D006801:Humans; D008689:Methacrylates; D011550:Pseudomonas aeruginosa / Q000302:isolation & purification; D012706:Serratia marcescens / Q000302:isolation & purification; D013210:Staphylococcus / Q000302:isolation & purification*
chemical_list: D008689:Methacrylates; C005044:hydroxyethyl methacrylate