A comparison of the reaction mechanisms of iron- and manganese-containing 2,3-HPCD: an important spin transition for manganese.
Homoprotocatechuate (HPCA) dioxygenases are enzymes that take part in the catabolism of aromatic compounds in the environment. They use molecular oxygen to perform the ring cleavage of ortho-dihydroxylated aromatic compounds. A theoretical investigation of the catalytic cycle for HPCA 2,3-dioxygenase isolated from Brevibacterium fuscum (Bf 2,3-HPCD) was performed using hybrid DFT with the B3LYP functional, and a reaction mechanism is suggested. Models of different sizes were built from the crystal structure of the enzyme and were used in the search for intermediates and transition states. It was found that the enzyme follows a reaction pathway similar to that for other non-heme iron dioxygenases, and for the manganese-dependent analog MndD, although with different energetics. The computational results suggest that the rate-limiting step for the whole reaction of Bf 2,3-HPCD is the protonation of the activated oxygen, with an energy barrier of 17.4 kcal/mol, in good agreement with the experimental value of 16 kcal/mol obtained from the overall rate of the reaction. Surprisingly, a very low barrier was found for the O-O bond cleavage step, 11.3 kcal/mol, as compared to 21.8 kcal/mol for MndD (sextet spin state). This result motivated additional studies of the manganese-dependent enzyme. Different spin coupling between the unpaired electrons on the metal and on the evolving substrate radical was observed for the two enzymes, and therefore the quartet spin state potential energy surface of the MndD reaction was studied. The calculations show a crossing between the sextet and the quartet surfaces, and it was concluded that a spin transition occurs and determines a barrier of 14.4 kcal/mol for the O-O bond cleavage, which is found to be the rate-limiting step in MndD. Thus the two 83% identical enzymes, using different metal ions as co-factors, were found to have similar activation energies (in agreement with experiment), but different rate-limiting steps.
Georgiev
Valentin
V
Department of Physics, Arrhenius Laboratories, Stockholm University, 106 91 Stockholm, Sweden. valyo@physto.se
Borowski
Tomasz
T
Blomberg
Margareta R A
MR
Siegbahn
Per E M
PE
D015102:3,4-Dihydroxyphenylacetic Acid / Q000737:chemistry*; D001951:Brevibacterium / Q000201:enzymology; D002384:Catalysis; D003198:Computer Simulation; D018360:Crystallography, X-Ray; D049308:Dioxygenases / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D008345:Manganese / Q000737:chemistry*; D008956:Models, Chemical; D008958:Models, Molecular; D015394:Molecular Structure; D010100:Oxygen / Q000737:chemistry; D011789:Quantum Theory; D013011:Sorbic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis; D013237:Stereoisomerism
C532463:1-carboxymethyl-4-hydroxy cis-muconic semialdehyde; D015102:3,4-Dihydroxyphenylacetic Acid; D008345:Manganese; D007501:Iron; D049308:Dioxygenases; C020055:3,4-dihydroxyphenylacetate 2,3-dioxygenase; D010100:Oxygen; D013011:Sorbic Acid