Cloning and differential expression of QM like protein homologue from tea [Camellia sinensis (L.) O. Kuntze].
The QM like protein gene encodes for ribosomal protein L10, which is implicated in tumor suppression, transcription factor regulation, and ribosome stability in yeast and mammals. Present study describes cloning of a full-length QM cDNA (CsQM) from tea leaves using differential display of mRNA followed by rapid amplification of cDNA ends. Expression of CsQM was studied in leaves of different stages of development and under various external cues. CsQM contained an open reading frame of 651 bases, encoding 216 amino acids. CsQM shared 71-87% and 85-91% identity at nucleotide and amino acid sequences, respectively with QM genes isolated from other plant species. During active-growth period of tea, higher expression was observed in apical buds that decreased gradually with increasing age of the leaf. During dormancy season, the expression of CsQM gene was severely down-regulated in all the leaves studied. CsQM transcript was found to be down regulated in response to drought stress and abscisic acid treatment but up-regulated by gibberellic acid treatment. A positive association of CsQM transcript abundance with active cellular growth suggested its role in plant growth and development.
Singh
Kashmir
K
Biotechnology Division, Institute of Himalayan Bioresource Technology, Palampur, HP, India.
Paul
Asosii
A
Kumar
Sanjay
S
Ahuja
Paramvir Singh
PS
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D001483:Base Sequence; D028241:Camellia sinensis / Q000235:genetics*; D003001:Cloning, Molecular; D055864:Droughts; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005875:Gibberellins / Q000494:pharmacology; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological / Q000187:drug effects
D005875:Gibberellins; D010940:Plant Proteins; D000040:Abscisic Acid