ET-Kyoto solution plus dibutyryl cyclic adenosine monophosphate is superior to University of Wisconsin solution in rat liver preservation.
ET-Kyoto solution (ET-K) is an extracellular-type organ preservation solution containing the cytoprotective disaccharide, trehalose. A previous study reported the supplement of dibutyryl cyclic adenosine monophosphate (db-cAMP) in conventional ET-K to attenuate lung ischemia-reperfusion injury. In this study, the efficacy of this modified ET-K for liver preservation was investigated by comparison with University of Wisconsin solution (UW). ET-K was supplemented with db-cAMP (2 mmol/L). Lewis rats were randomly assigned to two groups, and liver grafts were flushed and stored at 40C for 24 h with ET-K or UW before syngeneic liver transplantation. The graft function and histological changes at 4 h posttransplant as well as 7-day survival were evaluated. Recipient rat survival rate was significantly higher in the ET-K group than in the UW group. Preservation in ET-K resulted in a significant reduction in serum parenchymal transaminase level and promotion of bile production in comparison with UW. The serum hyaluronic acid level, an indicator of sinusoidal endothelial cell injury, was significantly lower after ET-K preservation than that in UW. Histologically, at 4 h after transplantation, the liver grafts preserved in UW solution demonstrated a greater degree of injury than those in ET-K, which appeared to be apoptosis, rather than necrosis. The continuity of the sinusoidal lining was better preserved in ET-K than in UW. In conclusion, ET-K supplemented with db-cAMP is superior to UW in rat liver preservation. This modified ET-K might therefore be a novel candidate for the procurement and preservation of multiple organs.
Zhao
Xiangdong
X
Department of Organ Preservation Technology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan.
Koshiba
Takaaki
T
Nakamura
Takayuki
T
Tsuruyama
Tatsuaki
T
Li
Ying
Y
Bando
Toru
T
Wada
Hiromi
H
Tanaka
Koichi
K
D000241:Adenosine; D000493:Allopurinol; D000818:Animals; D017209:Apoptosis; D001219:Aspartate Aminotransferases / Q000097:blood; D003994:Bucladesine; D005942:Gluconates; D005978:Glutathione; D006085:Graft Survival; D006895:Hydroxyethyl Starch Derivatives; D007328:Insulin; D016031:Liver Transplantation; D008297:Male; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions; D010710:Phosphates; D011887:Raffinose; D051381:Rats; D015427:Reperfusion Injury / Q000517:prevention & control; D014199:Trehalose
C087059:ET-Kyoto solution; D005942:Gluconates; D006895:Hydroxyethyl Starch Derivatives; D007328:Insulin; D019188:Organ Preservation Solutions; D010710:Phosphates; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D003994:Bucladesine; D014199:Trehalose; D001219:Aspartate Aminotransferases; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose