Antioxidant activity of diphenylpropionamide derivatives: synthesis, biological evaluation and computational analysis.
We report the synthesis, antioxidant and antiproliferative activity and a QSAR analysis of synthetic diphenylpropionamide derivatives. Synthesis of these compounds was achieved by direct condensation of 2,2- and 3,3-diphenylpropionic acid and appropriate amines using 1-propylphoshonic acid cyclic anhydride (PPAA) as catalyst. Compound structures were elucidated by NMR analysis and their melting points were measured. The in vitro antioxidant activity of these compounds was tested by evaluating the amount of scavenged ABTS radical and estimating ROS and NO production in LPS stimulated J774.A1 macrophages. All compounds were tested for their effect on viability of cells and results demonstrated that they are not toxic towards the cell lines used. The cytotoxic activity of all compounds was evaluated by a Brine Shrimp Test.
Urbani
Paolo
P
Department of Pharmaceutical Sciences, University of Salerno,Via Ponte don Melillo, 84084 Fisciano, (SA) Italy. purbani@unisa.it
Ramunno
Anna
A
Filosa
Rosanna
R
Pinto
Aldo
A
Popolo
Ada
A
Bianchino
Erminia
E
Piotto
Stefano
S
Saturnino
Carmela
C
De Prisco
Rocco
R
Nicolaus
Barbara
B
Tommonaro
Giuseppina
G
D000577:Amides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D019295:Computational Biology / Q000379:methods*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000166:cytology / Q000187:drug effects; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D011422:Propionates / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D000577:Amides; D000975:Antioxidants; D008070:Lipopolysaccharides; D011422:Propionates; D017382:Reactive Oxygen Species; D009569:Nitric Oxide