Monitoring singlet oxygen in situ with delayed chemiluminescence to deduce the effect of photodynamic therapy.
Singlet oxygen ((1)O(2)) is an important factor mediating cell killing in photodynamic therapy (PDT). We previously reported that chemiluminescence (CL) can be used to detect (1)O(2) production in PDT and linked the signal to the PDT-induced cytotoxicity in vitro. We develop a new CL detection apparatus to achieve in vivo measurements. The system utilizes a time-delayed CL signal to overcome the interference from scattered excitation light, thus greatly improving the accuracy of the detection. The system is tested on healthy skin of BALB/ca mouse for its feasibility and reliability. The CL measurement is made during a synchronized gating period of the irradiation light. After each PDT treatment and in situ CL measurement, the skin response is scored over a period of 2 weeks. A remarkable relationship is observed between the score and the CL, regardless of the PDT treatment protocol. Although there are many issues yet to be addressed, our results clearly demonstrate the feasibility of CL measurement during PDT and its potential for in vivo PDT dosimetry. This requires further investigations.
Wei
Yanchun
Y
South China Normal University, Ministry of Education Key Laboratory of Laser Life Science, Guangzhou 510631 China.
Xing
Da
D
Luo
Shiming
S
Xu
Wei
W
Chen
Qun
Q
D004867:Equipment Design; D019544:Equipment Failure Analysis; D008163:Luminescent Measurements / Q000295:instrumentation*; D010778:Photochemotherapy / Q000295:instrumentation* / Q000379:methods; D012680:Sensitivity and Specificity; D026082:Singlet Oxygen / Q000032:analysis*
D026082:Singlet Oxygen