title: Optimization of a laboratory-developed test utilizing roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species.
abstract: Nasal and perianal swab specimens were tested for detection of Staphylococcus aureus and vancomycin-resistant Enterococcus species (VRE) using a laboratory-developed real-time PCR test and microbiological cultures. The real-time PCR and culture results for S. aureus were similar. PCR had adequate sensitivity, but culture was more specific for the detection of VRE.
authors.affiliation: Evanston Northwestern Healthcare, Evanston, Illinois 60201, USA.
authors.forename: Maitry S
authors.initials: MS
authors.lastname: Mehta
authors.forename: Suzanne M
authors.initials: SM
authors.lastname: Paule
authors.forename: Donna M
authors.initials: DM
authors.lastname: Hacek
authors.forename: Richard B
authors.initials: RB
authors.lastname: Thomson
authors.forename: Karen L
authors.initials: KL
authors.lastname: Kaul
authors.forename: Lance R
authors.initials: LR
authors.lastname: Peterson
mesh_terms: D001003:Anal Canal / Q000382:microbiology; D001431:Bacteriological Techniques / Q000379:methods*; D016983:Enterococcus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D016106:Methicillin Resistance; D009666:Nose / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D020713:Vancomycin Resistance