title: Tissue-specific expression of ALA synthase-1 and heme oxygenase-1 and their expression in livers of rats chronically exposed to ethanol.
abstract: 5-Aminolevulinic acid synthase-1 (ALAS1) and heme oxygenase-1 (HO-1) are the rate-controlling enzymes for heme biosynthesis and degradation, respectively. Expression of these two genes showed tissue-specific expression pattern at both mRNA and protein levels in selected non-treated rat tissues. In the livers of rats receiving oral ethanol for 10 weeks, ALAS1 mRNA levels were increased by 65%, and the precursor and mature ALAS1 protein levels were increased by 1.8- and 2.3-fold, respectively, while no changes were observed in HO-1 mRNA and protein levels, compared with pair-fed controls. These results provide novel insights into the effects of chronic ethanol consumption on hepatic heme biosynthesis and porphyrias.
authors.affiliation: Liver-Biliary-Pancreatic Center, Carolinas Medical Center, 1000 Blythe Boulevard, Charlotte, NC 28203, USA. jianyu.zheng@carolinashealthcare.org <jianyu.zheng@carolinashealthcare.org>
authors.forename: Jianyu
authors.initials: J
authors.lastname: Zheng
authors.forename: Qing
authors.initials: Q
authors.lastname: Tian
authors.forename: Weihong
authors.initials: W
authors.lastname: Hou
authors.forename: John A
authors.initials: JA
authors.lastname: Watts
authors.forename: Laura W
authors.initials: LW
authors.lastname: Schrum
authors.forename: Herbert L
authors.initials: HL
authors.lastname: Bonkovsky
mesh_terms: D000624:5-Aminolevulinate Synthetase / Q000235:genetics / Q000378:metabolism*; D000428:Alcohol Drinking / Q000378:metabolism*; D000818:Animals; D000431:Ethanol / Q000008:administration & dosage / Q000633:toxicity*; D015870:Gene Expression / Q000187:drug effects; D006418:Heme / Q000096:biosynthesis; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D008297:Male; D011164:Porphyrias / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D012333:RNA, Messenger; D000431:Ethanol; D006418:Heme; D051547:Heme Oxygenase-1; D000624:5-Aminolevulinate Synthetase