title: Acceleration of biomimetic mineralization to apply in bone regeneration.
abstract: The delivery of growth factors and therapeutic drugs into bone defects is a major clinical challenge. Biomimetically prepared bone-like mineral (BLM) containing a carbonated apatite layer can be used to deliver growth factors and drugs in a controlled manner. In the conventional biomimetic process, BLM can be deposited on the biodegradable polymer surfaces by soaking them in simulated body fluid (SBF) for 16 days or more. The aim of this study was to accelerate the biomimetic process of depositing BML in the polymer surfaces. We accelerated the deposition of mineral on 3D poly(lactic-co-glycolic acid) (PLGA) porous scaffolds to 36-48 h by modifying the biomimetic process parameters and applying surface treatments to PLGA scaffolds. The BLM was coated on scaffolds after surface treatments followed by incubation at 37 degrees C in 15 ml of 5x SBF. We characterized the BLM created using the accelerated biomineralization process with wide angle x-ray diffraction (XRD), Fourier transform infrared (FTIR) microscopy, and scanning electron microscopy (SEM). The FTIR and XRD analyses of mineralized scaffolds show similarities between biomimetically prepared BLM, and bone bioapatite and carbonated apatite. We also found that the BLM layer on the surface of scaffolds was stable even after 21 days immersed in Tris buffered saline and cell culture media. This study suggests that BLM was stable for at least 3 weeks in both media, and therefore, BLM has a potential for use as a carrier for biological molecules for localized release applications as well as bone tissue engineering applications.
authors.affiliation: Department of Orthopaedics, University of Toledo, Toledo, OH 43614, USA.
authors.forename: A Champa
authors.initials: AC
authors.lastname: Jayasuriya
authors.forename: Chiragkumar
authors.initials: C
authors.lastname: Shah
authors.forename: Nabil A
authors.initials: NA
authors.lastname: Ebraheim
authors.forename: Ahalapitiya H
authors.initials: AH
authors.lastname: Jayatissa
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D002113:Calcification, Physiologic; D003460:Crystallization / Q000379:methods*; D019344:Lactic Acid / Q000737:chemistry*; D008422:Materials Testing; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D018786:Bone Substitutes; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid