title: Absence of antimutagenicity of Cochlospermum regium (Mart. and Schr.) Pilger 1924 by micronucleus test in mice.
abstract: Cochlospermum regium (Mart. and Schr.) Pilger, popularly known as "algodãozinho do campo", is a medicinal plant that grows in the Cerrado of Brazil. This plant has been used in traditional medicine against various diseases such as leucorrhoea, gastritis and ulcers. It has also been effective in treating skin problems like pimples, boils and blotches. In the present study, the in vivo antimutagenicity of aqueous extract of C. regium was evaluated. The Micronucleus Test was performed in polychromatic erythrocytes from Swiss male mice treated with one of the four doses of extract of the plant (19, 38, 76 and 114 mg.kg(-1) body weight), administered by intraperitonial injection (i.p.) simultaneously with cyclophosphamide (24 mg.kg(-1) b.w.) or mitomycin C (4 mg.kg(-1) b.w.). The cytotoxicity was evaluated by polychromatic and normochromatic erythrocytes ratio (PCE/NCE). The results showed no significant reduction of the micronucleated polychromatic erythrocytes frequency (P > 0.05). In conclusion, the data indicate that C. regium roots aqueous extract, for the conditions used, did not exhibit the antimutagenic effect.
authors.affiliation: Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, GO, 74001-97, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Andrade
authors.forename: D B
authors.initials: DB
authors.lastname: Santos
authors.forename: D B
authors.initials: DB
authors.lastname: Castro
authors.forename: L A
authors.initials: LA
authors.lastname: Guillo
authors.forename: L
authors.initials: L
authors.lastname: Chen-Chen
mesh_terms: D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D031212:Bixaceae / Q000737:chemistry*; D001854:Bone Marrow Cells / Q000187:drug effects*; D003520:Cyclophosphamide / Q000633:toxicity; D004912:Erythrocytes / Q000187:drug effects*; D008297:Male; D051379:Mice; D015162:Micronucleus Tests / Q000379:methods*; D016685:Mitomycin / Q000633:toxicity; D009153:Mutagens / Q000633:toxicity; D010936:Plant Extracts / Q000494:pharmacology
chemical_list: D016587:Antimutagenic Agents; D009153:Mutagens; D010936:Plant Extracts; D016685:Mitomycin; D003520:Cyclophosphamide