title: Intracellular applications of analytical SERS spectroscopy and multispectral imaging.
abstract: The aim of this tutorial review is to give an overview of the state of the art of intracellular applications of analytical SERS spectroscopy. We pay particular attention to nanoparticle-based SERS spectroscopy since this currently dominates the published literature on non-disturbing analysis of live cells. We describe recent advances in this domain due to the development of multispectral imaging and to the combined use of SERRS (surface-enhanced resonance Raman scattering) and fluorescence spectroscopy. Finally, a perspective view is given on the tip-based approaches like tip-enhanced Raman spectroscopy (TERS) which allow micrometric and nanometric resolution.
authors.affiliation: Université François-Rabelais, Faculté de Pharmacie, Focalisation magnétique d'agents anticancéreux, 31 avenue Monge, F-37200 Tours, France. chourpa@univ-tours.fr
authors.forename: Igor
authors.initials: I
authors.lastname: Chourpa
authors.forename: Franck H
authors.initials: FH
authors.lastname: Lei
authors.forename: Pierre
authors.initials: P
authors.lastname: Dubois
authors.forename: Michel
authors.initials: M
authors.lastname: Manfait
authors.forename: Ganesh D
authors.initials: GD
authors.lastname: Sockalingum
mesh_terms: D000818:Animals; D002451:Cell Compartmentation / Q000502:physiology*; D006801:Humans; D007424:Intracellular Fluid / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008839:Microelectrodes; D013050:Spectrometry, Fluorescence; D013059:Spectrum Analysis, Raman / Q000379:methods*