title: Amino-acid substitutions in acetylcholinesterase 1 involved in insecticide resistance in mosquitoes.
abstract: In natural populations of mosquitoes, high level of resistance to carbamates (CX) and organophosphates (OP) is provided by insensitive acetylcholinesterase (AChE1). Different alleles conferring resistance have been identified at the ace1 locus. They differ from the wild-type by only one amino-acid substitution. The comparison of the biochemical characteristics of mutated recombinant proteins and AChE1 in resistant mosquito extracts confirmed the role of each substitution in insensitivity. Selection of these different resistant alleles in field populations depends likely on the insecticides used locally. Theoretical modelling studies are initiated to develop novel strategies of mosquito control.
authors.affiliation: Institut des Sciences de l'Evolution (UM2-CNRS), Université Montpellier 2, Team Genetics of Adaptation, Laboratoire Génétique et Environnement, Place Eugène Bataillon, CC 065, Montpellier 05, France. alout@isem.univ-montp2.fr
authors.forename: H
authors.initials: H
authors.lastname: Alout
authors.forename: M
authors.initials: M
authors.lastname: Weill
mesh_terms: D000110:Acetylcholinesterase / Q000737:chemistry* / Q000235:genetics; D019943:Amino Acid Substitution; D000818:Animals; D009033:Culicidae / Q000201:enzymology*; D007305:Insecticide Resistance / Q000235:genetics*; D008958:Models, Molecular
chemical_list: D000110:Acetylcholinesterase