Effects of delays in peripheral blood processing, including cryopreservation, on detection of CD31 expression on naive CD4 T cells.
Delayed processing of peripheral blood or peripheral blood mononuclear cell isolation and cryopreservation can lead to the detection of somewhat higher levels of CD31 expression on naïve CD4 T cells by flow cytometry. These observations should be considered in the planning of multicenter clinical trials and in the interpretation of the results of functional studies.
Higgins
Jeanette
J
AIDS Monitoring Laboratory, Clinical Services Program, SAIC-Frederick, Inc, NCI Frederick, Frederick, Maryland 21702, USA.
Metcalf
Julia A
JA
Stevens
Randy A
RA
Baseler
Michael
M
Proschan
Michael
M
Lane
H Clifford
HC
Sereti
Irini
I
D000328:Adult; D001793:Blood Preservation; D001800:Blood Specimen Collection; D018791:CD4 Lymphocyte Count; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D015925:Cryopreservation; D006679:HIV Seropositivity / Q000276:immunology* / Q000821:virology; D006801:Humans; D008875:Middle Aged; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000276:immunology / Q000378:metabolism*; D013997:Time Factors
D019408:Platelet Endothelial Cell Adhesion Molecule-1