title: Environmental enrichment during early stages of life reduces the behavioral, neurochemical, and molecular effects of cocaine.
abstract: It is known that negative environmental conditions increase vulnerability to drugs, whereas little is known on whether positive environmental conditions such as enriched environments (EE) have protective effects against addiction. We have previously found that EE consisting of bigger cages containing several toys that were changed once per week reduce cocaine-induced increases in locomotor activity. Here, we also show that the rewarding effects of cocaine are blunted in mice reared from weaning to adulthood in EE compared to mice reared in standard environments (SE). In addition, although both EE and SE mice develop behavioral sensitization to cocaine, EE mice show less activation in response to repeated administration of cocaine injections and reduced responses to cocaine challenges. In vivo microdialysis experiments demonstrate that the protective effects of EE do not depend on reduced cocaine-induced increases in the dopamine levels in the ventral or dorsal striatum. On the other hand, they were associated with reduced cocaine-induced expression of the immediate early gene zif-268 in the nucleus accumbens (shell and core) of EE mice. Finally, basal levels of Delta-Fos B, a transcription factor known to be increased by sustained activation of striatal neurons, are higher in the striatum of EE compared to SE mice and repeated administration of cocaine increases Delta-Fos B levels in SE mice but decreases them in EE mice. Altogether our results demonstrate that exposure to complex environments during early stages of life produce dramatic changes in the striatum that result in reduced reactivity to drugs of abuse.
authors.affiliation: Institut de Physiologie et Biologie Cellulaires, University of Poitiers, CNRS, Poitiers, France.
authors.forename: Marcello
authors.initials: M
authors.lastname: Solinas
authors.forename: Nathalie
authors.initials: N
authors.lastname: Thiriet
authors.forename: Rana
authors.initials: R
authors.lastname: El Rawas
authors.forename: Virginie
authors.initials: V
authors.lastname: Lardeux
authors.forename: Mohamed
authors.initials: M
authors.lastname: Jaber
mesh_terms: D000818:Animals; D016739:Behavior, Addictive; D001522:Behavior, Animal; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D003042:Cocaine / Q000494:pharmacology*; D003213:Conditioning, Psychological; D003342:Corpus Striatum / Q000378:metabolism*; D004298:Dopamine / Q000378:metabolism; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D004777:Environment; D015870:Gene Expression / Q000187:drug effects; D006799:Housing, Animal; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000187:drug effects; D009714:Nucleus Accumbens / Q000378:metabolism*; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D000697:Central Nervous System Stimulants; D051766:Early Growth Response Protein 1; C486109:Egr1 protein, mouse; C483819:Fosb protein, mouse; D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D003042:Cocaine; D004298:Dopamine