Quantitative determination of singlet oxygen generated by excited state aromatic amino acids, proteins, and immunoglobulins.
Singlet oxygen quantum yields generated by excited state aromatic amino acids (tryptophan, tyrosine, phenylalanine), N-acetylated amino acids (N-acetyl-tryptophan, N-acetyl-tyrosine, N-acetyl-phenylalanine), and from selected proteins and immunoglobulins have been quantified by time-resolved phosphorescence measurements. A small, but significant, quantum yield found for proteins and immunoglobulins demonstrates that molecular oxygen can diffuse through the polypeptide matrix and can be sensitized by residues buried within the folds of protein structure.
Chin
Khin K
KK
Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095-1569, USA.
Trevithick-Sutton
Colleen C
CC
McCallum
Jeremy
J
Jockusch
Steffen
S
Turro
Nicholas J
NJ
Scaiano
J C
JC
Foote
Christopher S
CS
Garcia-Garibay
Miguel A
MA
D000418:Albumins / Q000737:chemistry; D024322:Amino Acids, Aromatic / Q000737:chemistry*; D007136:Immunoglobulins / Q000737:chemistry*; D008163:Luminescent Measurements; D010047:Ovalbumin / Q000737:chemistry; D010777:Photochemistry; D011506:Proteins / Q000737:chemistry*; D026082:Singlet Oxygen / Q000032:analysis / Q000737:chemistry*; D013055:Spectrophotometry, Infrared
D000418:Albumins; D024322:Amino Acids, Aromatic; D007136:Immunoglobulins; D011506:Proteins; D026082:Singlet Oxygen; D010047:Ovalbumin