CD26 expression on CD4+T cells in patients with cutaneous leishmaniasis.
Surrogate marker(s) of protection in human leishmaniasis is not well defined. In this study, T helper 1 (Th1) and Th2 cytokine profiles and CD26 expression on CD4(+) T cells in peripheral blood mononuclear cells of patients with healing or non-healing forms of cutaneous leishmaniasis (CL) stimulated with Leishmania antigens were assessed. The level of interferon (IFN)-gamma production was significantly higher in patients with healing or non-healing forms of CL than in healthy controls, but it was not significantly different between the two patient groups. The level of interleukin-5 production was significantly higher in patients with the non-healing form of CL than in the two other groups. There was a significant increase in the level of CD26 expression on CD4(+) T cells in patients with healing (P < 0.001) or non-healing (P = 0.025) forms of CL compared with the control group, but no significant difference was seen between the two patient groups. A weak positive correlation was seen between IFN-gamma production and CD26 expression on CD4(+) T cells of patients with the healing form of lesion (r = 0.54, P = 0.008), but this correlation was not observed in patients with the non-healing form of CL (r = 0.53, P = 0.078). Surface CD26 is not correlated with the clinical manifestation of CL or IFN-gamma production. Therefore, CD26 is not a surrogate marker for IFN-gamma production in CL.
Jafari-Shakib
R
R
National Cell Bank of Iran, Pasteur Institute, Tehran, Iran.
Ajdary
S
S
Amiri
Z Mohtasham
ZM
Mohammadi
A M
AM
Nourijelyani
K
K
Mortazavi
H
H
Shokrgozar
M A
MA
Nikbin
B
B
Khamesipour
A
A
D000328:Adult; D000818:Animals; D000953:Antigens, Protozoan; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D016022:Case-Control Studies; D049109:Cell Proliferation; D018819:Dipeptidyl Peptidase 4 / Q000032:analysis*; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D018320:Leishmania major / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology* / Q000473:pathology; D008297:Male; D012867:Skin / Q000469:parasitology; D018709:Statistics, Nonparametric
D000953:Antigens, Protozoan; D015848:Interleukin-5; D016753:Interleukin-10; D007371:Interferon-gamma; D018819:Dipeptidyl Peptidase 4