Multisignal regulation of the rat NMDA1 receptor subunit gene--a pivotal role of glucocorticoid-dependent transcription.
Although excess of glucocorticoid causes neuronal damage with cognitive disorders, the molecular mechanism for this remains unclear. In this study, we examined the effect of adrenal corticosteroids on the transcription of NMDA glutamate receptor subunit genes and Alzheimer disease-related genes such as amyloid precursor protein (APP), beta-site amyloid precursor protein-cleaving enzyme 1 (BACE1), and presenilin using neuronal cell lines in vitro. We found that synthetic glucocorticoid dexamethasone (dex) potently increased the promoter activity of NMDA1 and 2A subunit genes, but did not stimulate those of Alzheimer disease-related genes. The similar effect of dex was observed on intrinsic NMDA1 mRNA and protein expression. Furthermore, dex showed synergistic and additive effects with protein kinase A- and C-mediated signaling pathways, respectively. Finally, treatment of the Neuro2A cells, which express intrinsic glucocorticoid receptor, with dex significantly enhanced the glutamate-induced neurotoxicity. Our results suggest that glucocorticoid-induced neuronal damage may be, at least partly, attributable to enhanced expression of glutamate NMDA receptor with a resultant increase in the susceptibility of glutamate-induced excitotoxicity rather than to a direct effect of the hormone to the Alzheimer disease-related genes.
Jing
He
H
Department of Endocrinology, Metabolism, and Nephrology, Kochi Medical School, Kochi University, Kohasu, Oko-cho, Nankoku 783-8505, Japan.
Iwasaki
Yasumasa
Y
Nishiyama
Mitsuru
M
Taguchi
Takafumi
T
Tsugita
Makoto
M
Taniguchi
Yoshinori
Y
Kambayashi
Machiko
M
Hashimoto
Kozo
K
D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism; D000818:Animals; D015153:Blotting, Western; D045744:Cell Line, Tumor; D003001:Cloning, Molecular; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D003907:Dexamethasone / Q000009:adverse effects*; D004357:Drug Synergism; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000187:drug effects*; D005938:Glucocorticoids / Q000009:adverse effects*; D006801:Humans; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011493:Protein Kinase C / Q000378:metabolism; D051381:Rats; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*; D020218:Response Elements; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000685:Serum Amyloid A Protein / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D014158:Transcription, Genetic / Q000187:drug effects*
D017868:Cyclic AMP-Dependent Protein Kinases; D003907:Dexamethasone; D005938:Glucocorticoids; D011493:Protein Kinase C; D016194:Receptors, N-Methyl-D-Aspartate; D000685:Serum Amyloid A Protein; D013755:Tetradecanoylphorbol Acetate