Functional cloning by phage display.
This review focusses on the isolation of proteins from genomic or cDNA expression products libraries displayed on phage. The use of phage display is highlighted for the characterization of binding proteins with diverse biological functions. Phage display is compared with another strategy, the yeast two-hybrid method. The combination of both strategies is especially powerful to eliminate false positives and to get information on the biochemical functions of proteins.
Jestin
Jean-Luc
JL
URA CNRS 2128, Département de Biologie Structurale et Chimie, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris 15, France. jjestin@pasteur.fr
D000818:Animals; D003001:Cloning, Molecular; D015723:Gene Library; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D008024:Ligands; D011485:Protein Binding
D008024:Ligands