Reproductive and developmental toxicity screening study of 2,4-dinitrophenol in rats.
Rats were treated by gavage once daily with 2,4-dinitrophenol (DNP) at 0 (control), 3, 10, or 30 mg/kg bw. Males were dosed for 46 days, beginning 14 days before mating, and females were dosed for 40-47 days, from 14 days before mating to day 3 of lactation. No deaths were observed in males and females of any group. A significant decrease in body weight gain and significant increase in liver weight were found in males and females at 30 mg/kg bw/day. The number of live pups on postnatal days (PNDs) 0 and 4, live birth index, and body weight of live male and female pups on PNDs 0 and 1 were significantly lowered at 30 mg/kg bw/day. External and internal examinations of pups revealed no increased incidence of malformations in DNP-treated groups. On the basis of these findings, we concluded that DNP has general and reproductive/developmental toxicity, but not teratogenicity, under the present conditions. The NOAEL of DNP is considered to be 10 mg/kg bw/day in rats.
Takahashi
Mika
M
Division of Risk Assessment, Biological Safety Research Center, National Institute of Health Sciences, Tokyo 158-8501, Japan.
Sunaga
Masao
M
Hirata-Koizumi
Mutsuko
M
Hirose
Akihiko
A
Kamata
Eiichi
E
Ema
Makoto
M
D019297:2,4-Dinitrophenol / Q000633:toxicity*; D000014:Abnormalities, Drug-Induced; D000818:Animals; D000831:Animals, Newborn / Q000254:growth & development*; D001835:Body Weight; D005260:Female; D008099:Liver / Q000002:abnormalities / Q000187:drug effects; D008297:Male; D009929:Organ Size; D011247:Pregnancy; D051381:Rats; D012098:Reproduction / Q000187:drug effects*
D019297:2,4-Dinitrophenol