NADPH oxidase activation is required for migration by LIGHT in human monocytes.
Homologous to lymphotoxins, shows inducible expression, and competes with herpes simplex virus (HSV) glycoprotein D (gD) for herpes virus entry mediator (HVEM; TR2) (LIGHT), a ligand of herpes virus entry mediator (HVEM), increased reactive oxygen species (ROS) and enhanced the destruction of bacteria in human monocytes. In this study, rhLIGHT was found to increase the expression of the chemokine receptors, chemokine receptor 1 (CCR1) and CCR2, as well as to accelerate the migration activity of human monocytes. Additionally, rhLIGHT was found to increase ROS via NADPH oxidase p47(phox) phosphorylation, which was found to be required for LIGHT-induced NF-kappaB activation, CCR1 and CCR2 expression, migration and IL-8 and TNF-alpha production. Taken together, these results indicate that NADPH oxidase activation is required for rhLIGHT-induced migration in human monocytes.
Heo
Sook-Kyoung
SK
Cardiovascular Medical Research Center and Department of Prescriptionology, University of Dongguk, Gyeongju City, Gyeongbuk 780-714, Republic of Korea.
Yun
Hyun-Jeong
HJ
Park
Won-Hwan
WH
Park
Sun-Dong
SD
D002465:Cell Movement; D004789:Enzyme Activation; D006801:Humans; D020672:I-kappa B Proteins / Q000378:metabolism; D016209:Interleukin-8 / Q000378:metabolism; D009000:Monocytes / Q000187:drug effects / Q000201:enzymology / Q000276:immunology*; D019255:NADPH Oxidases / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D010766:Phosphorylation; D017382:Reactive Oxygen Species / Q000378:metabolism; D054389:Receptors, CCR1 / Q000378:metabolism; D054390:Receptors, CCR2 / Q000378:metabolism; D011994:Recombinant Proteins / Q000494:pharmacology; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14 / Q000494:pharmacology / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
C515300:CCR1 protein, human; C515334:CCR2 protein, human; D020672:I-kappa B Proteins; D016209:Interleukin-8; C000606204:NFKBIA protein, human; D017382:Reactive Oxygen Species; D054389:Receptors, CCR1; D054390:Receptors, CCR2; D011994:Recombinant Proteins; D053326:Tumor Necrosis Factor Ligand Superfamily Member 14; D014409:Tumor Necrosis Factor-alpha; D000072000:NF-KappaB Inhibitor alpha; D019255:NADPH Oxidases; C060505:neutrophil cytosolic factor 1