title: Derivation of human embryonic stem cells in xeno-free conditions.
abstract: Human embryonic stem cells (hESC) have the potential to treat a wide range of diseases. Currently, the use of existing hESC lines in human clinical applications is limited, as they are derived from blastocysts subjected to immunosurgery with animal derived antibodies, and are maintained on mouse embryonic feeder (MEF) cells, in the presence of either fetal calf serum (FCS) or on Matrigel or with conditioned media from MEFs. Successful derivation of hESCs in xeno-free conditions is crucial in advancing stem cell therapy applications. Two hESC lines, one from chromosomally abnormal embryos and another cell line from normal embryos from the inner cell mass of human blastocysts are derived using a culture media that had 20% serum replacement (SR) and human FGF2 on human foreskin fibroblasts as feeder cells. Derivation and characterization of such xenofree hESCs suitable for clinical studies is described in this chapter.
authors.affiliation: Stem Cells, Reprogenitics, West Orange, NJ, USA.
authors.forename: Mohan C
authors.initials: MC
authors.lastname: Vemuri
authors.forename: Tim
authors.initials: T
authors.lastname: Schimmel
authors.forename: Pere
authors.initials: P
authors.lastname: Colls
authors.forename: Santiago
authors.initials: S
authors.lastname: Munne
authors.forename: Jacques
authors.initials: J
authors.lastname: Cohen
mesh_terms: D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D012333:RNA, Messenger