Tear IgA-ELISA: a novel and sensitive method for diagnosis of ophthalmic cysticercosis.
For the first time, presence of locally secreted specific IgA antibodies in tear specimen from human with ophthalmic cysticercosis is documented in the present study. The ELISA using Taenia solium metacestode excretory secretory (ES) antigen demonstrated a diagnostic level of IgA antibodies in tears with 100% sensitivity (6 out of 6 confirmed cases of ophthalmic cysticercosis) whereas, 25 of 34 (73.52%) clinically suspected cases were diagnosed positive. The ELISA using T. solium metacestode somatic antigen detected a diagnostic titre of IgA antibody in tears with a sensitivity of 50% (3 out of 6 confirmed cases). The specificity of the tear IgAELISA using T. solium metacestode somatic and ES antigens is observed to be 94.87% and 92.3%, respectively. Overall in tears, the ELISA using T. solium metacestode ES antigens for detection of IgA antibodies shows a higher diagnostic efficiency (93.33%) compared to that using T. solium metacestode somatic antigen (88.88%). The sensitivities of the ELISA for detection of IgA antibodies in tears is observed to be higher than that for detection of IgG antibodies in serum using either somatic or ES antigens of the parasite.
Sahu
Priyadarshi S
PS
Department of Microbiology, Jawaharlal Institute of Postgraduate Medical Education and Research, Pondicherry, India. priyadarshi_sahu@yahoo.com
Parija
Subhash C
SC
Sahu
Promod K
PK
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000947:Antigens, Helminth; D002648:Child; D002675:Child, Preschool; D003551:Cysticercosis / Q000175:diagnosis*; D003552:Cysticercus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005128:Eye Diseases / Q000469:parasitology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A / Q000032:analysis*; D008297:Male; D008875:Middle Aged; D012680:Sensitivity and Specificity; D041201:Taenia solium / Q000276:immunology; D013666:Tears / Q000276:immunology*
D000947:Antigens, Helminth; D007070:Immunoglobulin A