Bam32: a novel mediator of Erk activation in T cells.
Bam32 (B lymphocyte adapter molecule of 32 kDa) is an adapter protein expressed in some hematopoietic cells including B and T lymphocytes. It was previously shown that Bam32-deficient mice have defects in various aspects of B cell activation including B cell receptor (BCR)-induced Erk activation, BCR-induced proliferation and T-independent antibody responses. In this study, we have examined the role of Bam32 in T cell activation using Bam32-deficient mice. By comparing CD4(+) T cells from lymph nodes of wild-type and Bam32-deficient mice, we found that Bam32 was required for optimal TCR-induced Erk activation, cytokine production, proliferation and actin-mediated spreading of CD4(+) T cells. These results indicate a novel pathway to Erk activation in T cells involving the adapter protein Bam32.
Sommers
Connie L
CL
Laboratory of Cellular and Molecular Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Gurson
Jordan M
JM
Surana
Rishi
R
Barda-Saad
Mira
M
Lee
Jan
J
Kishor
Aparna
A
Li
Wenmei
W
Gasser
Adam J
AJ
Barr
Valarie A
VA
Miyaji
Michihiko
M
Love
Paul E
PE
Samelson
Lawrence E
LE
D000199:Actins / Q000235:genetics / Q000276:immunology; D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000378:metabolism*; D002448:Cell Adhesion / Q000276:immunology; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism; D004789:Enzyme Activation / Q000235:genetics; D048049:Extracellular Signal-Regulated MAP Kinases / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D019169:Jurkat Cells; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction
D000199:Actins; D048868:Adaptor Proteins, Signal Transducing; D016207:Cytokines; C403524:Dapp1 protein, mouse; D008074:Lipoproteins; D011948:Receptors, Antigen, T-Cell; D048049:Extracellular Signal-Regulated MAP Kinases