Correlative analyses of notch signaling with resveratrol-induced differentiation and apoptosis of human medulloblastoma cells.
Altered Notch signaling seems linked with medulloblastoma (MB) formation and resveratrol exhibits anti-medulloblastoma effects. However, the influence of resveratrol in Notch signaling of MB cells has not been described. This issue was addressed here by checking Notch1 and Notch2 statuses in three MB cell lines with and without resveratrol treatment. Notch1 and Notch2 were detected in the cytoplasm of three cell lines under normal condition, which were up-regulated by resveratrol along with differentiation, apoptosis and enhanced Hes1 nuclear translocation. Nevertheless, blockage of Notch enzymatic cleavage with gamma-seacretase inhibitors, DAPT and L-685,458, neither interrupted resveratrol-caused cellular events nor affected MB cell growth. These results demonstrate that Notch signaling has little relevance with resveratrol-induced differentiation and apoptosis and may not be a universal critical factor of MB cells.
Wang
Qian
Q
Department of Cell Biology and Liaoning Laboratory of Cancer Genomics, College of Basic Medical Sciences, Dalian Medical University, 116044 Dalian, PR China.
Li
Hong
H
Liu
Nan
N
Chen
Xiao-Yan
XY
Wu
Mo-Li
ML
Zhang
Kai-Li
KL
Kong
Qing-You
QY
Liu
Jia
J
D021581:Active Transport, Cell Nucleus / Q000187:drug effects / Q000502:physiology; D053829:Amyloid Precursor Protein Secretases / Q000037:antagonists & inhibitors / Q000378:metabolism; D000970:Antineoplastic Agents / Q000494:pharmacology / Q000627:therapeutic use; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D017209:Apoptosis / Q000187:drug effects / Q000502:physiology; D051792:Basic Helix-Loop-Helix Proteins / Q000187:drug effects / Q000378:metabolism; D001932:Brain Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D018398:Homeodomain Proteins / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D008527:Medulloblastoma / Q000188:drug therapy / Q000378:metabolism*; D051881:Receptor, Notch1 / Q000187:drug effects / Q000378:metabolism; D051883:Receptor, Notch2 / Q000187:drug effects / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism*; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D000072056:Transcription Factor HES-1; D015854:Up-Regulation / Q000187:drug effects / Q000502:physiology
D053829:Amyloid Precursor Protein Secretases; D000970:Antineoplastic Agents; D000975:Antioxidants; D051792:Basic Helix-Loop-Helix Proteins; D004791:Enzyme Inhibitors; D018398:Homeodomain Proteins; D051881:Receptor, Notch1; D051883:Receptor, Notch2; D051880:Receptors, Notch; D000077185:Resveratrol; D013267:Stilbenes; D000072056:Transcription Factor HES-1; C497761:NOTCH1 protein, human; C497764:NOTCH2 protein, human; C078493:HES1 protein, human