[The role of Porphyromonas gingivalis invasion on E-selectin expression in endothelial cells].
OBJECTIVE
To evaluate the role of Porphyromonas gingivalis(P.gingivalis)ATCC 33277 and W83 invasion on the mRNA and protein expression of E-selectin in vascular endothelial cells.


METHODS
A cell culture model of human umbilical vein endothelial cells was used in vitro. E-selectin mRNA was measured by reverse transcription-polymerase chain reaction; the protein expression of E-selectin was detected by Western blot assay. The data was analyzed with SAS 8.12 software package for repeated measures ANOVA.


RESULTS
P.gingivalis not only enhanced mRNA expression of E-selectin, but also enhanced the protein level of E-selectin.Compared to the control, the relative mRNA expression of E-selectin after P.gingivalis ATCC 33277/W83 invasion at 4-hour, 8-hour, 24-hour or exposed to TNFalpha was 294.0%/326.4%(P<0.05),179.3%/224.6%(P<0.05),126.7%/128.3% and 365.5%(P<0.05), respectively. The relative protein expression of E-selectin was 295.4%/343.6% (P<0.05), 386.3%/394.3%(P<0.05), 238.7%/296.4% (P<0.05) and 413.8% (P<0.05), respectively. The effect of P.gingivalis virulent strain W83 was more strongly than that of avirulent strain ACTT 33277.


CONCLUSIONS
P.gingivalis plays an important role in the pathogenesis and progression of atherosclerosis partly through upregulation of E-selectin.
Zhang
Dong-Mei
DM
Department of Periodontology, School of Stomatology, China Medical University, Shenyang, Liaoning Province, China.
Pan
Ya-Ping
YP
Lin
Li
L
Zhao
Jian
J
Li
Chen
C
D050197:Atherosclerosis; D002478:Cells, Cultured; D019040:E-Selectin / Q000096:biosynthesis*; D042783:Endothelial Cells; D006801:Humans; D016966:Porphyromonas gingivalis / Q000472:pathogenicity*; D014409:Tumor Necrosis Factor-alpha
D019040:E-Selectin; D014409:Tumor Necrosis Factor-alpha