[Expression of bcl-2 and survivin gene products in different stages of vessel differentiation in hemangiomas].
OBJECTIVE
To investigate the relationship between cell apoptosis and regression process of hemangiomas.


METHODS
Fourty four hemangiomas and 19 vascular malformations, confirmed by pathology without any previous treatments were involved. Hemangiomas were classified into two groups by histological characters, among which 23 were in proliferating phase and 21 cases were in involuting phase. SABC immunohistochemistry was used to determine the expression of bcl-2 and survivin genes in vascular and endothelial cells,and 8 normal skin tissues were obtained from infants and children for control. The data was analyzed using Chi-square test with SPSS 11.0 software package.


RESULTS
Significant difference of bcl-2 expression in endothelial cells was detected between the proliferation and involuting phase of hemangiomas (P<0.01). There was no significant difference of survivin expression in endothelial cells between the proliferating and involuting phases of hemangiomas.


CONCLUSIONS
This study confirms high level of bcl-2 expression in vascular endothelial cells in proliferating phase of infantile hemangiomas in contrast to that in the involuting phase of hemangiomas, which indicates that bcl-2 might play a role in the later stage of angiogenesis. While high level of survivin expression in vascular endothelial cells in the proliferating phase and involuting phase without significant difference indicates that survivin might not play a role in the apoptosis of infantile hemangiomas.
Tan
Hai-Song
HS
Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, Shenyang, Liaoning Province, China.
Qin
Xing-Jun
XJ
Wang
Xu-Kai
XK
D017209:Apoptosis; D002454:Cell Differentiation; D002648:Child; D042783:Endothelial Cells; D006391:Hemangioma / Q000378:metabolism*; D006801:Humans; D007223:Infant; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D000077022:Survivin
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; D000077022:Survivin