Characteristics of hepatitis B virus genotype G coinfected with genotype H in chimeric mice carrying human hepatocytes.
Accumulated evidence indicated that hepatitis B virus genotype G (HBV/G) is present exclusively in coinfection with other HBV genotypes. In Mexico, HBV/G from 6 men who had sex with men were coinfected with HBV/H. Phylogenetically complete genomes of the 6 Mexican HBV/G strains were closely related to previous ones from the US/Europe. Using uPA/SCID mice with human hepatocytes, monoinfection with HBV/G did not result in detectable HBV DNA in serum, whereas superinfection with HBV/G at week 10 inoculated HBV/H when HBV/H DNA was elevated to >10(7) copies/mL has enhanced the replication of HBV/G. The HBV/G was enhanced in another 3 inoculated with a serum passage containing HBV/G with a trace of HBV/H. Coinfection of mice with HBV/G and H induced fibrosis in the liver. In conclusion, the replication of HBV/G can be enhanced remarkably when it is coinfected with HBV/H. Coinfection with HBV/G may be directly cytopathic in immunosuppressive conditions.
Tanaka
Yasuhito
Y
Department of Clinical Molecular Informative Medicine, Nagoya City University Graduate School of Medical Sciences, Nagoya, 467-8601 Japan.
Sanchez
Laura V
LV
Sugiyama
Masaya
M
Sakamoto
Tomoyuki
T
Kurbanov
Fuat
F
Tatematsu
Kanako
K
Roman
Sonia
S
Takahashi
Satoru
S
Shirai
Tomoyuki
T
Panduro
Arturo
A
Mizokami
Masashi
M
D000818:Animals; D001483:Base Sequence; D017690:Cell Transplantation; D002478:Cells, Cultured; D002678:Chimera; D005060:Europe; D005260:Female; D016679:Genome, Viral; D006509:Hepatitis B / Q000473:pathology / Q000821:virology*; D006515:Hepatitis B virus / Q000145:classification / Q000502:physiology*; D022781:Hepatocytes / Q000637:transplantation / Q000821:virology; D006801:Humans; D008103:Liver Cirrhosis / Q000473:pathology / Q000821:virology; D008297:Male; D008800:Mexico; D051379:Mice; D016513:Mice, SCID; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014779:Virus Replication