[Construction for detecting of human papillomaviruses types by using a multiplexed Luminex assay].
OBJECTIVE
To develop multiplex human papillomavirus genotyping, a quick and sensitive high-throughput procedure for the identification of multiple HPV genotypes by using a multiplexed Luminex array.


METHODS
Standard plasmids containing HPV genomic DNA were developed a standard multiplexed suspension array for detection 13 HPV types on Luminex analysis platform. the primer and probes were selected, HPV type-specific oligonucleotide probes were coupled to fluorescence-labeled polystyrene beads.


RESULTS
The one or two of mixed HPV standard plasmids were identified HPV type by using 13 HPV genetypes multiplexed suspension array, different HPV types can be correctly detected simultaneously. The positives of HPV clinical samples were detected by using this method, and the genetypes of samples by detecting were the same results with using BLAST in NCBI.


CONCLUSIONS
The proposed method allowed a high through-out, special, simple, rapid and economical identification of HPV DNA genotypes, It is expected to be an extremely useful tool in the identification of HPV genetypes for a lot of clinical samples.
Dang
Qian-li
QL
Department of Dermatology, Shannxi Provincial People's Hospital, Xi'an 710068, China. dangql@yahoo.com.cn
Chen
Chao
C
DU
Peng
P
DU
Yan-hua
YH
Feng
Jie
J
D017931:DNA Primers / Q000008:administration & dosage; D004279:DNA, Viral / Q000032:analysis; D005189:False Positive Reactions; D005456:Fluorescent Dyes; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods; D017720:Molecular Epidemiology; D021141:Nucleic Acid Amplification Techniques; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D015345:Oligonucleotide Probes / Q000008:administration & dosage*; D027383:Papillomaviridae / Q000235:genetics / Q000302:isolation & purification*; D053918:Papillomavirus Vaccines / Q000008:administration & dosage*; D011137:Polystyrenes; D011933:Reagent Kits, Diagnostic; D017422:Sequence Analysis, DNA / Q000379:methods*; D013045:Species Specificity
D017931:DNA Primers; D004279:DNA, Viral; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D053918:Papillomavirus Vaccines; D011137:Polystyrenes; D011933:Reagent Kits, Diagnostic