title: Functionally important interactions between the nucleotide-binding domains of an antigenic peptide transporter.
abstract: The transporter associated with antigen processing (TAP), an ABC transporter, pumps cytosolic peptides into the endoplasmic reticulum, where the peptides are loaded onto class I MHC molecules for presentation to the immune system. Transport is fueled by the binding of ATP to two cytosolic nucleotide-binding domains (NBDs) and ATP hydrolysis. We demonstrate biochemically that there are two electrostatic interactions across the interface between the two TAP NBDs and that these interactions are important for peptide transport. Notably, disrupting these interactions by mutagenesis does not greatly alter the ATP hydrolysis rate in an isolated NBD model system, suggesting that the interactions function at alternative stages in the transport cycle. The data support the general model for ABC transporters in which the NBDs form a tight, closed conformation during transport. Our results are discussed in relation to other ABC transporters that do or do not conserve potential interacting residues of opposite charges at the homologous positions.
authors.affiliation: Department of Molecular and Cellular Biology, Harvard University, 7 Divinity Avenue, Cambridge, Massachusetts 02138, USA.
authors.forename: Erik
authors.initials: E
authors.lastname: Procko
authors.forename: Rachelle
authors.initials: R
authors.lastname: Gaudet
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry*; D000818:Animals; D017951:Antigen Presentation; D001692:Biological Transport; D003600:Cytosol / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007107:Immune System / Q000378:metabolism; D008954:Models, Biological; D008958:Models, Molecular; D016296:Mutagenesis; D010455:Peptides / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D055672:Static Electricity
chemical_list: D010455:Peptides; D000255:Adenosine Triphosphate