Pyridoxine may protect the cerebellar granular cells against glutamate-induced toxicity.
In the present study, the possible protective effect of the pyridoxine against glutamate-induced neurotoxicity in cerebellar granular cell culture of rat pups is investigated for its therapeutic potential. Glutamate (10(-7) M) was administered to cerebellar granular cell cultures that were prepared from one-day-old Sprague-Dawley rats. The neuroprotective effect of pyridoxine was examined. Pyridoxine at the doses of 10(-8), 10(-7), 10(-6), and 10(-5) M was introduced into the culture flasks before inclusion of glutamate. Pyridoxine at the doses of 10(-8) M and 10(-7) M significantly reduced glutamate cytotoxicity. A 10(-7) M dose of pyridoxine proved to be more effective than a 10(-8) M dose. The present study demonstrates that pyridoxine may protect glutamate-induced neurotoxicity. Neuroprotective effect of pyridoxine, at least in part, may result from its anti-glutamatergic activity. Pyridoxine merits further investigation as a therapeutic option in hypoxic-ischemic brain injury.
Büyükokuroglu
Mehmet Emin
ME
Department of Pharmacology, Faculty of Medicine, Afyon Kocatepe University, Afyon, Turkey. memin@aku.edu.tr
Gepdiremen
Akcahan
A
Taştekin
Ayhan
A
Ors
Rahmi
R
D000818:Animals; D018929:Cell Culture Techniques; D002531:Cerebellum / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018698:Glutamic Acid / Q000008:administration & dosage / Q000633:toxicity*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D020258:Neurotoxicity Syndromes / Q000517:prevention & control*; D011736:Pyridoxine / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014803:Vitamin B Complex / Q000494:pharmacology*
D018696:Neuroprotective Agents; D014803:Vitamin B Complex; D018698:Glutamic Acid; D011736:Pyridoxine