title: DAZL binds to the transcripts of several Tssk genes in germ cells.
abstract: The Dazl gene encodes a germ-cell-specific RNA-binding protein which is essential for spermatogenesis. It has been proposed that this protein (DAZL) binds to RNA in the cytoplasm of germ cells and controls spermatogenesis. Using the specific nucleic acids associated with proteins (SNAAP) technique, we identified 17 target mRNAs bound by mDAZL. Among these transcripts, we focused on TSSK2, which encodes a testis-specific serine/threonine kinase. To date, five TSSK family members have been cloned, and all are exclusively expressed in the testis. We demonstrated that in addition to the TSSK1 3'UTR , the 3'UTRs of TSSKs 2 and 4 were bound by human and mouse DAZL, and that human DAZL (hDAZL) bound to the 3'UTR of human TSSK5 (hTSSK5). Our results suggest that the Dazl gene may play different roles in human and mouse spermatogenesis by regulating different members of the downstream gene family.
authors.affiliation: Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, 610041, PR China.
authors.forename: Mei
authors.initials: M
authors.lastname: Zeng
authors.forename: Wenqian
authors.initials: W
authors.lastname: Deng
authors.forename: Xinying
authors.initials: X
authors.lastname: Wang
authors.forename: Weimin
authors.initials: W
authors.lastname: Qiu
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Liu
authors.forename: Huaqin
authors.initials: H
authors.lastname: Sun
authors.forename: Dachang
authors.initials: D
authors.lastname: Tao
authors.forename: Sizhong
authors.initials: S
authors.lastname: Zhang
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Ma
mesh_terms: D020413:3' Untranslated Regions / Q000378:metabolism; D000818:Animals; D001665:Binding Sites; D005854:Germ Cells / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000201:enzymology / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; C508515:DAZL protein, mouse; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C475219:Tsk1 protein, mouse; D017346:Protein Serine-Threonine Kinases; C504028:TSSK4 protein, human; C529882:Tssk2 protein, mouse; C529883:Tssk4 protein, mouse