Quadruplex PCR for simultaneous detection of serotype, biotype, toxigenic potential, and central regulating factor of Vibrio cholerae.
A quadruplex PCR was developed for the simultaneous detection of genes specific for Vibrio cholerae O1 and/or O139 serogroup (wbe and/or wbf), cholera toxin A subunit (ctxA), toxin-coregulated pilus (tcpA), and central regulating protein ToxR (toxR) in a single tube reaction. This is a simple, rapid, and accurate approach for the detection of toxigenic V. cholerae O1 and/or O139 and can prevent the rapid spread of the disease by early detection.
Khuntia
Hemant Kumar
HK
Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar, Orissa, India.
Pal
Bibhuti Bhusan
BB
Chhotray
Guru Prasada
GP
D001426:Bacterial Proteins / Q000235:genetics; D002771:Cholera / Q000382:microbiology; D002772:Cholera Toxin / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D036781:Fimbriae Proteins / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014157:Transcription Factors / Q000235:genetics; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics*; D041242:Vibrio cholerae O139 / Q000145:classification* / Q000235:genetics*
D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C085807:TcpA protein, Vibrio cholerae; D014157:Transcription Factors; C499324:toxR protein, Vibrio cholerae; D036781:Fimbriae Proteins; D002772:Cholera Toxin