title: Adsorption of tributyltin by tributyltin resistant marine Pseudoalteromonas sp. cells.
abstract: The isolate, Pesudoalteromonas sp. TBT1, could grow to overcome the toxicity of tributyltin chloride (TBTCl) up to 30 microM in the absence of Cl(-) in the medium until the cells reached an exponential phase of growth. The viability, however, was reduced after the cells reached a stationary phase. The degradation products, such as dibutyltin (DBT) and monobutyltin (MBT), were not detected in the growth medium, indicating that the isolate has no ability to degrade TBT into less toxic DBT and MBT. Up to about 10(7.5) TBT molecules were adsorbed by a single cell. The observation of morphological changes with an electron microscope showed that the cell surface became wrinkled after exposure to the lethal concentration of 10 mM TBTCl. These results indicate that the resistance of the isolate toward the toxicity of TBTCl is not related to the unique cell surface, which seems to play an important role in preventing the diffusion of TBTCl into the cytoplasm.
authors.affiliation: Graduate School of Maritime Sciences, Kobe University, 5-1-1, Fukae, Higashinada, Kobe 658-0022, Japan. hmimura@maritime.kobe-u.ac.jp
authors.forename: Haruo
authors.initials: H
authors.lastname: Mimura
authors.forename: Ryusei
authors.initials: R
authors.lastname: Sato
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Furuyama
authors.forename: Akira
authors.initials: A
authors.lastname: Taniike
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yagi
authors.forename: Kazutoshi
authors.initials: K
authors.lastname: Yoshida
authors.forename: Akira
authors.initials: A
authors.lastname: Kitamura
mesh_terms: D000327:Adsorption; D024881:Drug Resistance, Bacterial; D008855:Microscopy, Electron, Scanning; D031161:Pseudoalteromonas / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D014220:Trialkyltin Compounds / Q000493:pharmacokinetics* / Q000633:toxicity; D014874:Water Pollutants, Chemical / Q000493:pharmacokinetics* / Q000633:toxicity
chemical_list: D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin