title: In situ nuclear organization of regulatory machinery.
abstract: Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
authors.affiliation: Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA.
authors.forename: Shirwin M
authors.initials: SM
authors.lastname: Pockwinse
authors.forename: Sayyed K
authors.initials: SK
authors.lastname: Zaidi
authors.forename: Ricardo F
authors.initials: RF
authors.lastname: Medina
authors.forename: Rachit
authors.initials: R
authors.lastname: Bakshi
authors.forename: Krishna P
authors.initials: KP
authors.lastname: Kota
authors.forename: Syed A
authors.initials: SA
authors.lastname: Ali
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Young
authors.forename: Jeffery A
authors.initials: JA
authors.lastname: Nickerson
authors.forename: Amjad
authors.initials: A
authors.lastname: Javed
authors.forename: Martin
authors.initials: M
authors.lastname: Montecino
authors.forename: Andre J
authors.initials: AJ
authors.lastname: van Wijnen
authors.forename: Jane B
authors.initials: JB
authors.lastname: Lian
authors.forename: Janet L
authors.initials: JL
authors.lastname: Stein
authors.forename: Gary S
authors.initials: GS
authors.lastname: Stein
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism / Q000648:ultrastructure; D036681:Fluorescence Recovery After Photobleaching; D005786:Gene Expression Regulation; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism / Q000648:ultrastructure; D008856:Microscopy, Fluorescence / Q000379:methods