Adsorption of plasmid DNA on anion exchange chromatography media.
Anion exchange chromatography (AEC) is a useful and effective tool for DNA purification, but due to average pore sizes between 40 and 100 nm most AEC resins lack truly useful binding capacities for plasmid DNA (pDNA). Equilibrium binding capacities and uptake kinetics of AEC media including conventional media (Source 30 Q, Q Sepharose HP), a polymer grafted medium (Fractogel EMD DEAE (M)), media with large pores (Celbeads DEAE, PL SAX 4000 A 30 microm) and a monolithic medium (CIM-DEAE) were investigated by batch uptake or shallow bed experiments at two salt concentrations. Theoretical and experimental binding capacities suggest that the shape of the pDNA molecule can be described by a rod with a length to diameter ratio of 20:1 and that the molecule binds in upright position. The arrangement of DNA like a brush at the surface can be considered as entropy driven, kind of self-assembly process which is inherent to highly and uniformly charged DNA molecules. The initial phase of adsorption is very fast and levels off, associated with a change in mass transfer mechanism. Feed concentrations higher than 0.1 mg/mL pDNA pronounce this effect. Monolithic media showed the fastest adsorption rate and highest binding capacity with 13 mg pDNA per mL.
Tarmann
Christina
C
Department of Biotechnology, University of Applied Life Science and Natural Resources Vienna, Vienna, Austria.
Jungbauer
Alois
A
D000327:Adsorption; D000837:Anion Exchange Resins / Q000737:chemistry*; D002852:Chromatography, Ion Exchange / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000302:isolation & purification; D004058:Diffusion; D004983:Ethanolamines / Q000737:chemistry*; D007700:Kinetics; D008956:Models, Chemical; D010316:Particle Size; D016062:Porosity; D013499:Surface Properties
D000837:Anion Exchange Resins; D004983:Ethanolamines; D004247:DNA; C007369:2-diethylaminoethanol