Discovery of a modified transcription factor endowing yeasts with organic-solvent tolerance and reconstruction of an organic-solvent-tolerant Saccharomyces cerevisiae strain.
Organic-solvent tolerance in Saccharomyces cerevisiae strain KK-211, which was first isolated as an organic-solvent-tolerant strain, depends on point mutation (R821S) of the transcription factor Pdr1p. The integration of the PDR1 R821S mutation into wild-type yeast results in organic-solvent tolerance, and the PDR1 R821S mutant can reduce carbonyl compounds in organic solvents.
Matsui
Ken
K
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
Teranishi
Shinya
S
Kamon
Shohei
S
Kuroda
Kouichi
K
Ueda
Mitsuyoshi
M
D000935:Antifungal Agents / Q000494:pharmacology; D003513:Cycloheximide / Q000494:pharmacology; D004268:DNA-Binding Proteins; D025141:Drug Resistance, Fungal; D015966:Gene Expression Regulation, Fungal; D009930:Organic Chemicals / Q000494:pharmacology*; D017354:Point Mutation; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D012997:Solvents / Q000494:pharmacology*; D015534:Trans-Activators / Q000235:genetics*; D014157:Transcription Factors
D000935:Antifungal Agents; D004268:DNA-Binding Proteins; D009930:Organic Chemicals; C085480:PDR1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D012997:Solvents; D015534:Trans-Activators; D014157:Transcription Factors; D003513:Cycloheximide