Inhibition of proliferation in colon cancer cell lines and harmful enzyme activity of colon bacteria by Bifidobacterium adolescentis SPM0212.
In this study, we assessed the anticancer activity and bacterial enzyme inhibition of Bifidobacterium adolescentis SPM0212. B. adolescentis SPM0212 inhibited the proliferation of three human colon cancer cell lines: HT-29, SW 480, and Caco-2. SPM0212 also dose-dependently inhibited TNF-á production and changes in cellular morphology. B. adolescentis SPM0212 inhibited harmful fecal enzymes, including â-glucuronidase, â-glucosidase, tryptophanase, and urease. Thus, B. adolescentis SPM0212 exerts an anticancer effect and inhibits harmful fecal enzymes.
Kim
Yuna
Y
Department of Pharmacy, Sahmyook University, Seoul, Korea.
Lee
Dokyung
D
Kim
Donghyun
D
Cho
Jooyong
J
Yang
Jaewook
J
Chung
Myungjun
M
Kim
Kyungjae
K
Ha
Namjoo
N
D000328:Adult; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000627:therapeutic use; D044466:Asian People; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D001483:Base Sequence; D001644:Bifidobacterium / Q000145:classification / Q000235:genetics; D018938:Caco-2 Cells; D049109:Cell Proliferation / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D044602:Cellulases / Q000037:antagonists & inhibitors / Q000378:metabolism; D003106:Colon / Q000382:microbiology*; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D005243:Feces / Q000382:microbiology; D005966:Glucuronidase / Q000037:antagonists & inhibitors / Q000378:metabolism; D019073:HT29 Cells; D006801:Humans; D007723:Korea; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D019936:Probiotics / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017207:Rats, Sprague-Dawley; D014368:Tryptophanase / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014510:Urease / Q000037:antagonists & inhibitors / Q000378:metabolism
D000970:Antineoplastic Agents; D001426:Bacterial Proteins; D004791:Enzyme Inhibitors; D014409:Tumor Necrosis Factor-alpha; D044602:Cellulases; D005966:Glucuronidase; D014510:Urease; D014368:Tryptophanase