[Preparation and characterization of the monoclonal antibodies to PTD-bcr/abl].
OBJECTIVE
To Preparation and characterization the monoclonal antibody (mAb)to PTD-bcr/abl fusion protein.


METHODS
The PTD-bcr/abl fusion protein was expressed in E.coli. BALB/c mice were immunized with specific PTD-bcr/abl. The splenic cells of the mice were fused with Sp2/0 mouse myeloma cells. The hybridoma culture supernatants were screened by indirect ELISA. The property of the antibody was identified by Western blot and immunohistochemistry.


RESULTS
The PTD-bcr/abl fusion protein and its mAb were obtained. mAb against PTD-bcr/abl displayed strong specifility and high affinity.


CONCLUSIONS
An anti-PTD-bcr/abl fusion protein mAb with high affinity was abtained, which could be applied to the research of PTD-bcr/abl.
Chen
Ren-an
RA
Department of Haematology of Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, China. chen-renan@163.com
Liu
Li
L
Jiang
Shan-shan
SS
Liang
Ying-min
YM
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000917:Antibody Formation; D000918:Antibody Specificity; D003001:Cloning, Molecular; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D016044:Fusion Proteins, bcr-abl / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C
D000911:Antibodies, Monoclonal; D016044:Fusion Proteins, bcr-abl