title: Distribution of obestatin and ghrelin in human tissues: immunoreactive cells in the gastrointestinal tract, pancreas, and mammary glands.
abstract: Obestatin and ghrelin are two peptides derived from the same prohormone. It is well established that ghrelin is produced by endocrine cells in the gastric mucosa. However, the distribution of human obestatin immunoreactive cells is not thoroughly characterized. A polyclonal antibody that specifically recognizes human obestatin was produced. Using this antibody and a commercial antibody vs ghrelin, the distribution of obestatin and ghrelin immunoreactive cells was determined in a panel of human tissues using immunohistochemistry. The two peptides were detected in the mucosa of the gastrointestinal tract, from cardia to ileum, and in the pancreatic islets. Interestingly, epithelial cells in the ducts of mammary glands showed distinct immunoreactivity for both ghrelin and obestatin. By double immunofluorescence microscopy, it was shown that all detected cells were immunoreactive for both peptides. Furthermore, the subcellular localization of obestatin and ghrelin was essentially identical, indicating that obestatin and ghrelin are stored in the same secretory vesicles.
authors.affiliation: Department of Medical Sciences, Section of Endocrine Oncology, University Hospital, SE-751 85 Uppsala, Sweden. jan.saras@medsci.uu.se
authors.forename: Malin
authors.initials: M
authors.lastname: Grönberg
authors.forename: Apostolos V
authors.initials: AV
authors.lastname: Tsolakis
authors.forename: Linda
authors.initials: L
authors.lastname: Magnusson
authors.forename: Eva T
authors.initials: ET
authors.lastname: Janson
authors.forename: Jan
authors.initials: J
authors.lastname: Saras
mesh_terms: D041981:Gastrointestinal Tract / Q000378:metabolism*; D054439:Ghrelin / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D042361:Mammary Glands, Human / Q000378:metabolism*; D009928:Organ Specificity; D010179:Pancreas / Q000378:metabolism*
chemical_list: D054439:Ghrelin