Establishment of a new model for culturing rabbit osteoblasts in vitro.
To establish an experimental model for culturing rabbit osteoblasts in vitro, the osteoblasts were isolated from the calvarial bone of a 15-day old rabbit using a method of culturing the bone pieces in a medium after they had been digested by an enzyme for 15 min. The acquired cells were assayed by cell morphology, alkaline phosphatase activity and production of a mineralized matrix. The results showed that the cells had the morphologic characteristics and some biological behaviours of osteoblasts. Based on the primary isolation of osteoblasts from bone and combining digestion with explants, a novel model for culturing rabbit osteoblasts in vitro was established, which is easy, efficient and effective. This model can be used in many studies of osteogenesis mechanisms and bone replacement materials.
Cao
Xian-Ying
XY
Science and Engineering College of Hainan University, Key Laboratory of National Ministry of Education: Research and Application of Tropical Aquatic and Terrestrial Biological Resources, Haikou 570228, People's Republic of China. cxying 02@163.com
Yin
Mei-Zhen
MZ
Zhang
Li-Na
LN
Li
Shi-Pu
SP
Cao
Yang
Y
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D048429:Cell Size; D002478:Cells, Cultured; D006801:Humans; D023421:Models, Animal; D010006:Osteoblasts / Q000166:cytology* / Q000502:physiology*; D011817:Rabbits; D012886:Skull / Q000166:cytology* / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*