title: [Experimental study of hepatic bloodflow to the effect of cryoablation].
abstract: OBJECTIVE
  To investigate the bloodflow of hepetatic artery to the effect of cryoablation.
  
  
  METHODS
  Twenty dogs were divided into four groups randomly according to hepatic artery blood flow: completely occlusion of the hepatic artery(A), partial occlusion of the hepatic artery(B), increasing the hepatic artery blood flow(C) ,and no occlusion(D). Two areas of necrosis near portal hepatis (a)and far from it (b)were created in each of the 20 dog livers. Ultrasound was used to measure the blood flow. After cryoablation, the animals were euthanized, the livers were examined and the cryoablation areas were separated into two halves the maximum diameter of necrosis was assessed by vital staining for nicotinamide adenine dinucleotide (NADH) diaphorase and Triphenyl tetrazolium chloride (TTC) staining.
  
  
  RESULTS
  The peak speed and volflow of hepatic artery from group A to group D measured by ultrasound were 0 cm/s and 0 mL/min, (22.8+/-0.6) cm/s and (31.4+/-0.9) mL/min, (99.6+/-1.6) cm/s and (70.4+/-1.5) mL/min and (69.5+/-0.7) cm/s and (52.6+/-1.2) mL/min, respectively. The mean longest diameter of ice-ball in areas a from group A to group D were (4.5+/-0.4) cm,(3.5+/-0.1) cm,(2.0+/-0.3) cm,(2.6+/-0.2) cm and the areas b were (4.6+/-0.3) cm,(3.6+/-0.2) cm,(1.9+/-0.1) cm,(2.6+/-0.2) cm; the mean longest necroseis diameter in TTC staining in areas a were (4.0+/-0.3) cm,(2.7+/-0.2) cm, (2.0+/-0.2) cm, (2.3+/-0.1) cm, and in areas b were (3.9+/-0.3) cm, (2.6+/-0.3) cm, (2.1+/-0.1) cm,(2.5+/-0.2) cm; the mean longest necroseis diameter in NADH staining in areas a were (4.1+/-0.4) cm,(2.6+/-0.2) cm,(2.1+/-0.2) cm,(2.3+/-0.3) cm, in areas b were (4.0+/-0.3) cm, (2.7+/-0.3) cm,(2.1+/- 0.1) cm,(2.4+/-0.2) cm.
  
  
  CONCLUSIONS
  The bloodflow of hepetatic artery can affect the cryoablation rate. When the bloodflow increases, the cryoablation rate decreases.
authors.affiliation: Intervention Radiation Department, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Guo
authors.forename: Hong
authors.initials: H
authors.lastname: Ni
authors.forename: Yong hua
authors.initials: Yh
authors.lastname: Hu
authors.forename: Wen ge
authors.initials: Wg
authors.lastname: Xing
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
mesh_terms: D000818:Animals; D001783:Blood Flow Velocity; D003452:Cryosurgery; D004285:Dogs; D005260:Female; D006499:Hepatic Artery / Q000601:surgery*; D008099:Liver / Q000098:blood supply* / Q000473:pathology / Q000601:surgery*; D008297:Male; D009336:Necrosis; D011897:Random Allocation