title: Activation of NF-kappaB in lymphocytes and increase in serum immunoglobulin in hyperthyroidism: possible role of oxidative stress.
abstract: This study evaluated oxidative stress, serum IgM and IgG, and nuclear factor (NF)-kappaB signaling in lymphocytes of hyperthyroidism patients. GSH content in lymphocytes was significantly lower and serum malondialdehyde, IgM and IgG levels were significantly higher in hyperthyroidism as compared to controls. In lymphocytes, the NF-kappaB signaling pathway was studied by western blot analysis of p65 and p-IkappaBalpha. Density of p-IkappaBalpha and p65 (in nuclear fraction) was significantly higher in hyperthyroidism as compared to controls. The density of p-IkappaBalpha and p65 had significant positive correlation with serum malondialdehyde level and negative correlation with lymphocyte GSH level in hyperthyroid cases. The serum IgG and IgM levels were correlated significantly with density of p-IkappaBalpha and p65. As immunoglobulin production is regulated by the NF-kappaB pathway, we conclude that the oxidative stress-induced activation of the NF-kappaB pathway might play a role in the rise of serum immunoglobulin level in hyperthyroidism.
authors.affiliation: Department of Biochemistry, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Pondicherry-6, India.
authors.forename: D N
authors.initials: DN
authors.lastname: Nandakumar
authors.forename: Bidhan Chandra
authors.initials: BC
authors.lastname: Koner
authors.forename: R
authors.initials: R
authors.lastname: Vinayagamoorthi
authors.forename: Nivedita
authors.initials: N
authors.lastname: Nanda
authors.forename: V S
authors.initials: VS
authors.lastname: Negi
authors.forename: Kalyan
authors.initials: K
authors.lastname: Goswami
authors.forename: Zachariah
authors.initials: Z
authors.lastname: Bobby
authors.forename: Abdoul
authors.initials: A
authors.lastname: Hamide
mesh_terms: D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006980:Hyperthyroidism / Q000097:blood / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000097:blood / Q000235:genetics; D007075:Immunoglobulin M / Q000097:blood / Q000235:genetics; D008214:Lymphocytes / Q000276:immunology*; D008297:Male; D026901:Membrane Transport Proteins / Q000097:blood / Q000235:genetics; D008875:Middle Aged; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018384:Oxidative Stress / Q000235:genetics / Q000276:immunology*; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology*; D014284:Triiodothyronine / Q000097:blood
chemical_list: D007074:Immunoglobulin G; D007075:Immunoglobulin M; D026901:Membrane Transport Proteins; D016328:NF-kappa B; C086860:glutathione transporter; D014284:Triiodothyronine