title: A rapid antibody-based test for Sec-2, a marker for the short arm of chromosome 2 of rye (2RS).
abstract: A simple monoclonal antibody-based screening test has been developed for the presence of translocations of the short arm of chromosome 2 of rye (2RS) with wheat chromosome 2B. 2RS encodes a set of about three polypeptides known as Mr 75 000 gamma-secalins. Use of the antibody test for these secalins enabled screening of several hundred seeds per day. The antibody could readily distinguish 2BL-2RS translocations and 2R substitutions from 1BL-1RS translocations or nontranslocation wheats. Use of the antibody in analysis of segregating progeny for Sec-2 in several wheat backgrounds was successful. Results with a selection of the seed population were checked using protein gel electrophoresis, with 100% correct confirmation. Key words : rye, wheat, seed proteins, translocation, diagnostic test.
authors.forename: J H
authors.initials: JH
authors.lastname: Skerritt
authors.forename: R B
authors.initials: RB
authors.lastname: Gupta
authors.forename: J L
authors.initials: JL
authors.lastname: Andrews
authors.forename: F L
authors.initials: FL
authors.lastname: Stoddard
authors.forename: N K
authors.initials: NK
authors.lastname: Howes