Asymmetric tethering of flat and curved lipid membranes by a golgin.
Golgins, long stringlike proteins, tether cisternae and transport vesicles at the Golgi apparatus. We examined the attachment of golgin GMAP-210 to lipid membranes. GMAP-210 connected highly curved liposomes to flatter ones. This asymmetric tethering relied on motifs that sensed membrane curvature both in the N terminus of GMAP-210 and in ArfGAP1, which controlled the interaction of the C terminus of GMAP-210 with the small guanine nucleotide-binding protein Arf1. Because membrane curvature constantly changes during vesicular trafficking, this mode of tethering suggests a way to maintain the Golgi architecture without compromising membrane flow.
Drin
Guillaume
G
Institut de Pharmacologie Moléculaire et Cellulaire, Université de Nice Sophia Antipolis and CNRS, 660 route des lucioles, 06560 Valbonne, France.
Morello
Vincent
V
Casella
Jean-François
JF
Gounon
Pierre
P
Antonny
Bruno
B
D020823:ADP-Ribosylation Factor 1 / Q000378:metabolism; D001665:Binding Sites; D002460:Cell Line; D003598:Cytoskeletal Proteins; D020690:GTPase-Activating Proteins / Q000378:metabolism; D006056:Golgi Apparatus / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007425:Intracellular Membranes / Q000737:chemistry* / Q000378:metabolism; D008081:Liposomes; D008563:Membrane Lipids / Q000737:chemistry*; D009687:Nuclear Proteins / Q000737:chemistry* / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism
C503237:ARFGAP1 protein, human; C503240:ARFGAP3 protein, human; D003598:Cytoskeletal Proteins; D020690:GTPase-Activating Proteins; D008081:Liposomes; D008563:Membrane Lipids; D009687:Nuclear Proteins; D011994:Recombinant Proteins; C109331:TRIP11 protein, human; D020823:ADP-Ribosylation Factor 1