Functional analysis of the essential GTP-binding-protein-coding gene cgtA of Vibrio cholerae.
The cgtA gene, coding for the conserved G protein CgtA, is essential in bacteria. In contrast to a previous report, here we show by using genetic analysis that cgtA is essential in Vibrio cholerae even in a Delta relA background. Depletion of CgtA affected the growth of V. cholerae and rendered the cells highly sensitive to the replication inhibitor hydroxyurea. Overexpression of V. cholerae CgtA caused distinct elongation of Escherichia coli cells. Deletion analysis indicated that the C-terminal end of CgtA plays a critical role in its proper function.
Shah
Sangita
S
Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata 700 032, India.
Das
Bhabatosh
B
Bhadra
Rupak K
RK
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D020043:Genes, Essential / Q000235:genetics* / Q000502:physiology; D006918:Hydroxyurea / Q000494:pharmacology; D008855:Microscopy, Electron, Scanning; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D009154:Mutation; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D014734:Vibrio cholerae / Q000166:cytology / Q000235:genetics* / Q000648:ultrastructure
D001426:Bacterial Proteins; D019384:Nucleic Acid Synthesis Inhibitors; C108621:CgtA protein, bacteria; D020559:Monomeric GTP-Binding Proteins; D006918:Hydroxyurea