title: Evaluation of seven tests for diagnosis of human brucellosis in an area where the disease is endemic.
abstract: The results of seven serologic tests for diagnosis of human brucellosis were evaluated. The titrated Rose Bengal test, microagglutination test, microtiter-adapted Coombs test, and immunocapture-agglutination test (Brucellacapt) were positive for all sera from patients with acute brucellosis. The immunoglobulin G (IgG), IgM, and IgA commercial enzyme immunoassays (ELISAs) failed to show specific antibodies in 3 patients, 10 patients, and 1 patient, respectively. The sensitivity of ELISA is not higher than that of conventional tests.
authors.affiliation: Microbiology Laboratory Hospital Severo Ochoa (Leganés), Madrid, Spain. mcongocri@hotmail.com
authors.forename: M Concepción
authors.initials: MC
authors.lastname: Gómez
authors.forename: José A
authors.initials: JA
authors.lastname: Nieto
authors.forename: Carmen
authors.initials: C
authors.lastname: Rosa
authors.forename: Paloma
authors.initials: P
authors.lastname: Geijo
authors.forename: M Angeles
authors.initials: MA
authors.lastname: Escribano
authors.forename: Amparo
authors.initials: A
authors.lastname: Muñoz
authors.forename: Clara
authors.initials: C
authors.lastname: López
mesh_terms: D000372:Agglutination Tests; D002002:Brucella / Q000276:immunology; D002006:Brucellosis / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology; D003298:Coombs Test; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D012680:Sensitivity and Specificity; D012698:Serologic Tests