PiRNA-like RNAs in the marsupial Monodelphis domestica identify transcription clusters and likely marsupial transposon targets.
PIWI-interacting RNAs (piRNAs) are a recently discovered class of small noncoding RNAs that have been detected in human, mouse, rat, zebrafish, and Drosophila genomes. We have utilized a size-directed small-RNA cloning procedure to clone and map more than 300 candidate piRNA-like small RNAs in the genome of the marsupial species Monodelphis domestica. Our results are consistent with those from other species in that the piRNA-like candidate sequences range in size from 28 to 31 nucleotides, show a pronounced preference for uridine at the 5' end, are transcribed from a few large clusters, appear to target transposons, and display virtually no sequence conservation.
Devor
Eric J
EJ
Molecular Genetics and Biophysics, Integrated DNA Technologies, 1710 Commercial Park, Coralville, IA 52241, USA. rdevor@idtdna.com
Huang
Lingyan
L
Samollow
Paul B
PB
D000818:Animals; D001483:Base Sequence; D002999:Clone Cells; D004251:DNA Transposable Elements / Q000235:genetics*; D008297:Male; D008969:Molecular Sequence Data; D048309:Monodelphis / Q000235:genetics*; D005810:Multigene Family; D034741:RNA, Small Interfering / Q000235:genetics*; D012984:Software; D014158:Transcription, Genetic
D004251:DNA Transposable Elements; D034741:RNA, Small Interfering