title: Molecular characterization of Enterococcus faecalis N06-0364 with low-level vancomycin resistance harboring a novel D-Ala-D-Ser gene cluster, vanL.
abstract: Enterococcus faecalis N06-0364, exhibiting a vancomycin MIC of 8 microg/ml, was found to harbor a novel D-Ala-D-Ser gene cluster, designated vanL. The vanL gene cluster was similar in organization to the vanC operon, but the VanT serine racemase was encoded by two separate genes, vanTm(L) (membrane binding) and vanTr(L) (racemase).
authors.affiliation: National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
authors.forename: David A
authors.initials: DA
authors.lastname: Boyd
authors.forename: Barbara M
authors.initials: BM
authors.lastname: Willey
authors.forename: Darlene
authors.initials: D
authors.lastname: Fawcett
authors.forename: Nazira
authors.initials: N
authors.lastname: Gillani
authors.forename: Michael R
authors.initials: MR
authors.lastname: Mulvey
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D013293:Enterococcus faecalis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D005798:Genes, Bacterial; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family; D009876:Operon; D010453:Peptide Synthases / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D020713:Vancomycin Resistance / Q000235:genetics
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D004269:DNA, Bacterial; D010453:Peptide Synthases; C108395:VanC protein, Enterococcus