Hymenobacter psychrotolerans sp. nov., isolated from the Qinghai--Tibet Plateau permafrost region.
A psychrotolerant bacterium, designated strain Tibet-IIU11T, was isolated from the Qinghai--Tibet Plateau permafrost region, China. A taxonomic study was conducted using a polyphasic approach, with determination of physiological and biochemical properties and phylogenetic analysis based on 16S rRNA gene sequences. The novel isolate was found to belong to the genus Hymenobacter and was distinct from the recognized species of this genus. The major fatty acids were iso-C15 : 0 (24.5 %), summed feature 3 (C16 : 1omega7c and/or iso C15 : 0 2-OH; 20.0 %), summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B; 14.0 %), C16 : 1omega5c (12.7 %) and anteiso-C15 : 0 (8.4 %). Phosphatidylethanolamine and an unknown aminophospholipid were predominant in the polar lipid profile. The quinone system consisted exclusively of menaquinone MK-7 and sym-homospermidine was the major polyamine present. These chemotaxonomic traits are in good agreement with the characteristics of the genus Hymenobacter. The assignment of the novel isolate to this genus was further supported by a DNA G+C content of 60 mol%. On the basis of the polyphasic evidence presented in this study, it is proposed that strain Tibet-IIU11T (=DSM 18569T=CGMCC 1.6365T) represents a novel species, Hymenobacter psychrotolerans sp. nov.
Zhang
Gaosen
G
School of Life Sciences, Key Lab of Arid and Grassland Agroecology of MOE, Lanzhou University, Lanzhou 730000, PR China.
Niu
Fujun
F
Busse
Hans-Jürgen
HJ
Ma
Xiaojun
X
Liu
Wei
W
Dong
Maoxing
M
Feng
Huyuan
H
An
Lizhe
L
Cheng
Guodong
G
D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D003080:Cold Temperature; D041964:Cytophagaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D019015:Geologic Sediments / Q000382:microbiology*; D046448:Ice Cover / Q000382:microbiology*; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S