Identification and diversity of putative aminoglycoside-biosynthetic aminotransferase genes from deep-sea environmental DNA.
We obtained DNA fragments encoding putative aminotransferases possibly involved in the biosynthesis of aminoglycoside antibiotics from deep-sea sediments of the northwest Pacific Ocean by nested PCR, and 34 individual genes (total 89 clones) were identified. About half of the deep-sea sequences showed similarity with genes of known aminoglycoside-producers, but others were deep-sea specific genes. Furthermore, we found that temperature-gradient gel electrophoresis (TGGE) can be an effective tool in the analysis of these DNA fragments.
Aoki
Rie
R
Department of Chemistry, College of Humanities and Sciences, Nihon University, Setagaya-ku, Tokyo 156-8550, Japan.
Nagaya
Atsushi
A
Arakawa
Shizuka
S
Kato
Chiaki
C
Tamegai
Hideyuki
H
D000595:Amino Acid Sequence; D000617:Aminoglycosides / Q000096:biosynthesis*; D004247:DNA / Q000235:genetics* / Q000302:isolation & purification*; D004586:Electrophoresis; D014644:Genetic Variation; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010140:Pacific Ocean; D010802:Phylogeny; D013696:Temperature; D000637:Transaminases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D000617:Aminoglycosides; D004247:DNA; D000637:Transaminases