In situ nuclear organization of regulatory machinery.
Regulatory machinery for gene expression, replication, and repair are architecturally organized in nuclear microenvironments. This compartmentalization provides threshold concentrations of macromolecules for the organization and assembly of regulatory complexes for combinatorial control. A mechanistic under standing of biological control requires the combined application of molecular, cellular, biochemical, and in vivo genetic approaches. This chapter provides methodologies to characterize nuclear organization of regulatory machinery by in situ immunofluorescence microscopy.
Pockwinse
Shirwin M
SM
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA, USA.
Zaidi
Sayyed K
SK
Medina
Ricardo F
RF
Bakshi
Rachit
R
Kota
Krishna P
KP
Ali
Syed A
SA
Young
Daniel W
DW
Nickerson
Jeffery A
JA
Javed
Amjad
A
Montecino
Martin
M
van Wijnen
Andre J
AJ
Lian
Jane B
JB
Stein
Janet L
JL
Stein
Gary S
GS
D000818:Animals; D018929:Cell Culture Techniques; D002453:Cell Cycle; D002467:Cell Nucleus / Q000378:metabolism* / Q000648:ultrastructure; D002478:Cells, Cultured; D002875:Chromosomes / Q000378:metabolism / Q000648:ultrastructure; D036681:Fluorescence Recovery After Photobleaching; D005786:Gene Expression Regulation; D006801:Humans; D007382:Intermediate Filaments / Q000378:metabolism / Q000648:ultrastructure; D008856:Microscopy, Fluorescence / Q000379:methods