Preparation, characterization and evaluation of Marsupsin-phospholipid complex.
The aim of this research was to formulate Marsupsin-phospholipid complex (M-P Complex) in attempt to increase the bioavailability of marsupsin and to characterize this new formulation along with its evaluation. Marsupsin-phospholipid complex was formulated by mechanical dispersion method. In this new formulation, complex formation was confirmed by carrying out transmission electron microscopy (TEM), IR, (1)H-NMR and RP-HPLC analysis. TEM showed M-P Complex diameter range of 0.05-0.5 microm. The entrapment efficiency of M-P Complex was found to be 44%. In vitro release study revealed its first order release profile. Mean blood serum concentration vs time curve of marsupsin was of first order after oral administration of M-P Complex in albino rabbits which clearly showed remarkably increased bioavailability of M-P Complex than standardized marsupsin. The average value of C(max) and T(max) of M-P Complex were found to be 3.02 mg/ml and 10.2 h, respectively. Hence the findings demonstrate that complexing marsupsin with phospholipids results in better oral bioavailability and improved biological response than free form of standardized marsupsin.
Sikarwar
Mukesh S
MS
Department of Pharmacognosy and Phytochemistry, B. R. Nahata College of Pharmacy, Mhow-Neemuch Road, Mandsaur 458001, India. mukeshsikarwar@gmail.com
Sharma
Shalini
S
Jain
Amit K
AK
Parial
S D
SD
D000818:Animals; D001572:Benzofurans / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D003408:Cresols / Q000008:administration & dosage* / Q000737:chemistry / Q000493:pharmacokinetics*; D003920:Diabetes Mellitus / Q000097:blood / Q000188:drug therapy*; D004058:Diffusion; D004337:Drug Carriers / Q000737:chemistry*; D004339:Drug Compounding / Q000379:methods; D004353:Drug Evaluation, Preclinical; D007004:Hypoglycemic Agents / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D008422:Materials Testing; D010743:Phospholipids / Q000737:chemistry*; D011817:Rabbits; D051381:Rats; D016896:Treatment Outcome
D001572:Benzofurans; D003408:Cresols; D004337:Drug Carriers; D007004:Hypoglycemic Agents; D010743:Phospholipids; C083154:marsupsin