The AOC promoter of tomato is regulated by developmental and environmental stimuli.
The allene oxide cyclase (AOC) catalyzes the formation of cis-(+)-12-oxophytodienoic acid, an intermediate in jasmonate biosynthesis and is encoded by a single copy gene in tomato. The full length AOC promoter isolated by genome walk contains 3600 bp. Transgenic tomato lines carrying a 1000 bp promoter fragment and the full length promoter, respectively, in front of the beta-glucuronidase (GUS)-encoding uidA gene and several tobacco lines carrying the full length tomato AOC promoter before GUS were used to record organ- and tissue-specific promoter activities during development and in response to various stimuli. High promoter activities corresponding to immunocytochemically detected occurrence of the AOC protein were found in seeds and young seedlings and were confined to the root tip, hypocotyl and cotyledons of 3-d-old seedlings. In 10-d-old seedlings promoter activity appeared preferentially in the elongation zone. Fully developed tomato leaves were free of AOC promoter activity, but showed high activity upon wounding locally and systemically or upon treatment with JA, systemin or glucose. Tomato flowers showed high AOC promoter activities in ovules, sepals, anthers and pollen. Most of the promoter activity patterns found in tomato with the 1000 bp promoter fragment were also detected with the full length tomato AOC promoter in tobacco during development or in response to various stimuli. The data support a spatial and temporal regulation of JA biosynthesis during development and in response to environmental stimuli.
Stenzel
Irene
I
Leibniz Institute of Plant Biochemistry, Department of Natural Product Biotechnology, Weinberg 3, D-06120 Halle (Saale), Germany.
Hause
Bettina
B
Proels
Reinhard
R
Miersch
Otto
O
Oka
Mariko
M
Roitsch
Thomas
T
Wasternack
Claus
C
D003001:Cloning, Molecular; D035264:Flowers / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D005638:Fruit / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D019746:Intramolecular Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D011401:Promoter Regions, Genetic / Q000235:genetics*; D036226:Seedlings / Q000201:enzymology / Q000235:genetics / Q000254:growth & development
D019746:Intramolecular Oxidoreductases; C021377:hydroperoxide isomerase