Identification of up-regulated genes in malignant glioma with subtraction hybridization: preliminary screening studies.
OBJECTIVE
This investigation is intended to obtain differentially expressed genes related to human malignant glioma using Subtractive hybridization.


METHODS
Subtractive hybridization is potentially faster methods for identifying differentially expressed genes associated with a particular disease state. We identified 7 over-expressed genes which were not homologous to any of the known genes in the Genbank database.


RESULTS
Using semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), the mRNA expression levels of these 7 genes were higher in human glioblastomas tissue than in non-tumor brain tissue. In order to learn more about the expression profile of these genes, RT-PCR was performed using various commercially available human carcinoma cell lines. Some of these new genes were over-expressed in human glioma cell line, but not the expressed in other human cancer cell line.


CONCLUSIONS
Theses cloned new genes may play a role in brain tumorigenesis. Further studies including verification of oncogene, cancer protein, and glioblastoma induction in animal model are needed.
Cho
Yong Jae
YJ
Department of Neurosurgery, Ewha Womans University Mok-Dong Hospital, 911-1 Mok-dong, Yangchun-gu, Seoul 158-710, Korea. yongcho@ewha.ac.kr
D000368:Aged; D001932:Brain Neoplasms / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000235:genetics* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015854:Up-Regulation / Q000235:genetics
D012333:RNA, Messenger