Soluble gp130, an antagonist of IL-6 transsignaling, is elevated in uveitis aqueous humor.
OBJECTIVE
To determine the concentrations of soluble gp130, a natural antagonist of IL-6 transsignaling, in the serum and aqueous humor (AqH) of patients with uveitis.


METHODS
Serum was obtained from the peripheral blood of patients with active uveitis and healthy control subjects. AqH samples were collected from patients with active uveitis and those without uveitis who were undergoing routine cataract surgery. Samples were centrifuged and the cell-free supernatant frozen at -80 degrees C. Concentrations of sgp130, sIL-6R, and IL-6 were determined by a sandwich ELISA or multiplex bead immunoassay, using standard curves of known concentrations of recombinant cytokines.


RESULTS
Serum concentrations of sgp130 were not significantly different between control individuals and patients with active anterior uveitis, regardless of the degree of intraocular inflammation cells. By contrast, the concentration of sgp130 in AqH was very low in patients with no or little inflammation, but increased significantly with disease severity. The greatest elevations of AqH sgp130 were found in patients with the highest cellular activity. Simultaneous measurement of IL-6, sIL-6R, and sgp130 revealed a high degree of correlation between the levels of these molecules, especially for sIL-6R and sgp130.


CONCLUSIONS
Soluble gp130 is increased in the AqH of patients with active uveitis. It is likely that sgp130 partially inhibits the process of IL-6 transsignaling during inflammation. However, the concentration found is still far below that in serum, suggesting that increasing the level of sgp130 further may assist in reducing the inflammatory changes induced by IL-6 transsignaling.
Simon
Diana
D
Institute of Biomedical Research, MRC Centre for Immune Regulation, Division of Immunity and Infection, Medical School, The University of Birmingham, Birmingham, United Kingdom.
Denniston
Alastair K O
AK
Tomlins
Paul J
PJ
Wallace
Graham R
GR
Rauz
Saaeha
S
Salmon
Mike
M
Murray
Philip I
PI
Curnow
S John
SJ
D001082:Aqueous Humor / Q000276:immunology / Q000502:physiology*; D002387:Cataract Extraction; D050822:Cytokine Receptor gp130 / Q000097:blood / Q000502:physiology*; D015796:HLA-B27 Antigen / Q000276:immunology; D006801:Humans; D007249:Inflammation / Q000517:prevention & control; D015850:Interleukin-6 / Q000097:blood / Q000502:physiology*; D019947:Receptors, Interleukin-6 / Q000097:blood / Q000276:immunology; D012016:Reference Values; D015398:Signal Transduction; D014606:Uveitis, Anterior / Q000097:blood* / Q000276:immunology; D000093242:Interleukin-6 Inhibitors
D050822:Cytokine Receptor gp130; D015796:HLA-B27 Antigen; D015850:Interleukin-6; D019947:Receptors, Interleukin-6; D000093242:Interleukin-6 Inhibitors