title: The ability of HDL to inhibit VCAM-1 expression and oxidized LDL uptake is impaired in renal patients.
abstract: OBJECTIVE
  This study examines the ability of HDL from hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients to suppress the expression of adhesion molecules in endothelial cells (ICAM-1, VCAM-1) and in monocytes (LFA-1, VLA-4) and to inhibit the uptake of oxidized LDL by macrophages.
  
  
  METHODS
  Gene expression and the uptake of oxidized LDL were determined in 12 HD patients, 12 CAPD patients and 14 healthy volunteers.
  
  
  RESULTS
  HDL from renal patients were less effective than control lipoproteins in reducing VCAM-1 expression. HDL from CAPD patients inhibited LFA-1 expression to the highest extent. The ability of HDL from renal patients to reduce oxidized LDL uptake was lower compared to control group.
  
  
  CONCLUSIONS
  Decreased ability of HDL to suppress expression of VCAM-1 in endothelial cells and the uptake of oxidized LDL by macrophages can be one of the risk factors for atherosclerosis development in patients with renal failure.
authors.affiliation: Department of General Biochemistry, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland. Aleksandra.Jurek@LICR.uu.se
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Jurek
authors.forename: Bohdan
authors.initials: B
authors.lastname: Turyna
authors.forename: Piotr
authors.initials: P
authors.lastname: Kubit
authors.forename: Andrzej
authors.initials: A
authors.lastname: Klein
mesh_terms: D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D040881:CD11a Antigen / Q000378:metabolism; D008076:Cholesterol, HDL / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D039441:Integrin alpha4 / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000628:therapy; D008077:Lipoproteins, LDL / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory; D012333:RNA, Messenger / Q000378:metabolism; D006435:Renal Dialysis; D020298:U937 Cells; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
chemical_list: D040881:CD11a Antigen; D008076:Cholesterol, HDL; D008077:Lipoproteins, LDL; D016169:Lymphocyte Function-Associated Antigen-1; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; C099252:oxidized low density lipoprotein; D018799:Intercellular Adhesion Molecule-1; D039441:Integrin alpha4