title: Functional outcome of bone marrow stem cells (CD45(+)/CD34(-)) after cell therapy in acute spinal cord injury: in exercise training and in sedentary rats.
abstract: BACKGROUND
  Cell therapy and exercise training may be options for spinal cord regeneration. Our objective was to evaluate the functional effects of autologous bone marrow stem cell (CD45(+)/CD34(-)) transplantation in acute spinal cord injury in exercise training and in sedentary rats.
  
  
  METHODS
  Fifty-five adult male Wistar rats underwent spinal cord contusion by Impactor (NYU). Locomotor rating scale was performed every 48 hours for 48 days. Animals with scores < or = 12 were randomly divided into 4 groups: sedentary without parenchymal cell infusion; sedentary with parenchymal cell infusion; swimming training without parenchymal cell infusion; and swimming training with parenchymal cell infusion. Bone marrow stem cells were isolated by puncture-aspiration of the bone marrow and density gradient (d = 1.077). The animals underwent a 60-minute swimming session 6 times/week supporting an overload of 3% of body weight for 6 consecutive weeks. Comparisons between the groups in relation to differences between the beginning to the end of scores used the nonparametric Bonferroni test and post-hoc Mann-Whitney U test to identify significance.
  
  
  RESULTS
  Forty-two rats that obtained scores < or = 12 underwent therapy with 9 animals in each of the 4 groups as completors (n = 36). There was significance (P < or = .008) for sedentary without parenchymal cell infusion vs swimming training with parenchymal cell infusion.
  
  
  CONCLUSIONS
  The combination of bone marrow stem cell therapy (CD45(+)/CD34(-)) and exercise training resulted in significant functional improvement in acute spinal cord injury.
authors.affiliation: Cellular Culture Experimental Laboratory, PUCPR Curitiba Brazil. katherinecarv@gmail.com
authors.forename: K A T
authors.initials: KA
authors.lastname: Carvalho
authors.forename: R C
authors.initials: RC
authors.lastname: Cunha
authors.forename: E N
authors.initials: EN
authors.lastname: Vialle
authors.forename: R
authors.initials: R
authors.lastname: Osiecki
authors.forename: G H G
authors.initials: GH
authors.lastname: Moreira
authors.forename: R B
authors.initials: RB
authors.lastname: Simeoni
authors.forename: J C
authors.initials: JC
authors.lastname: Francisco
authors.forename: L C
authors.initials: LC
authors.lastname: Guarita-Souza
authors.forename: L
authors.initials: L
authors.lastname: Oliveira
authors.forename: L
authors.initials: L
authors.lastname: Zocche
authors.forename: M
authors.initials: M
authors.lastname: Olandoski
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D004195:Disease Models, Animal; D008297:Male; D009043:Motor Activity; D010805:Physical Conditioning, Animal; D051381:Rats; D017208:Rats, Wistar; D013119:Spinal Cord Injuries / Q000534:rehabilitation / Q000601:surgery*; D033581:Stem Cell Transplantation / Q000379:methods*; D013594:Syringes; D016896:Treatment Outcome