Improving the cell affinity of a poly(D,L-lactide) film modified by grafting collagen via a plasma technique.
Poly(D,L-lactide) films were surface-modified by grafting collagen via NH(3) plasma to improve cell affinity. The modified films were characterized by IR analysis, contact angle measurement, SEM analysis and collagen quantity determination. It was demonstrated that -NH(2) and collagen were incorporated into the surface of PDLLA films. The hydrophilicity of the PDLLA film increased after NH(3) plasma treatment, but decreased with further collagen modification. More collagen was incorporated into the PDLLA films by a grating method as compared to that with an anchorage treatment. L929 fibroblast cells were used to evaluate the cell affinity of the modified films and control. It was shown that PDLLA films surface-modified by grafting collagen via NH(3) plasma more efficiently enhanced the cells attachment and proliferation than those films modified by collagen anchorage or only NH(3) plasma treatment.
Zhao
Jian-Hao
JH
Department of Material Science and Engineering, College of Science and Engineering, Jinan University, 510632, Guangzhou, People's Republic of China. zjh629@126.com
Wang
Jue
J
Tu
Mei
M
Luo
Bing-Hong
BH
Zhou
Chang-Ren
CR
D000327:Adsorption; D000818:Animals; D002448:Cell Adhesion / Q000502:physiology*; D002460:Cell Line; D049109:Cell Proliferation; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D005347:Fibroblasts / Q000502:physiology*; D006358:Hot Temperature; D008422:Materials Testing; D008567:Membranes, Artificial; D051379:Mice; D011091:Polyesters / Q000737:chemistry*; D011485:Protein Binding; D023822:Tissue Engineering / Q000379:methods*
D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D011091:Polyesters; C033616:poly(lactide); D003094:Collagen