[In vitro growth kinetics of two Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) clones in myocardial cells from rodents of different susceptibility].
In vitro growth kinetics of two Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae) clones in myocardial cells from rodents of different susceptibility. Two Trypanosoma cruzi isolates, TCR-4 from Costa Rica and UES-1 from El Salvador, were studied in vitro to compare their infectivity or resistance and intracellular replication in myocardial cells in three strains of mice and rats: NGP white mice, C3 H mice and Sprague Dowley rats. Myocardial cells were cultured on coverslips at 37 degrees C in a humid 10% CO2 atmosphere and then infected at a ratio of one tripomastigote per cell. Samples were studied after 24, 72, 96 and 120 h of infection to determine parasite infection capacity and intracellular multiplication. Both parasites had the highest infection capacity in C3 H mice, followed by NGP mice cells with a very low infection rate. Lastly, almost no Trypanosoma cruzi multiplication was observed in Sprague Dowley rats, suggesting a strong natural resistance in this animal to both strains of the parasite. The UES-1 isolate presented higher multiplication and greater invasion than the TCR-4 strain, showing greater virulence of UES-1 in heart cells, at least in vitro.
Angel
Rafael-Oswaldo
RO
Departamento de Microbiología, Facultad de Medicina, Universidad de El Salvador. rangel@elsalvador.com
Chinchilla
Misael
M
Marta Guerrero
Olga
O
Castro
Alfredo
A
D000818:Animals; D002478:Cells, Cultured; D002999:Clone Cells / Q000469:parasitology; D003364:Costa Rica; D004544:El Salvador; D007700:Kinetics; D051379:Mice; D008809:Mice, Inbred C3H; D032383:Myocytes, Cardiac / Q000469:parasitology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014349:Trypanosoma cruzi / Q000235:genetics / Q000254:growth & development* / Q000472:pathogenicity; D014774:Virulence