title: Circadian genomics of the chick pineal gland in vitro.
abstract: BACKGROUND
  Chick pinealocytes exhibit all the characteristics of a complete circadian system, comprising photoreceptive inputs, molecular clockworks and an easily measured rhythmic output, melatonin biosynthesis. These properties make the in vitro pineal a particularly useful model for exploring circadian control of gene transcription in a pacemaker tissue, as well as regulation of the transcriptome by primary inputs to the clock (both photic and noradrenergic).
  
  
  RESULTS
  We used microarray analysis to investigate the expression of approximately 8000 genes within cultured pinealocytes subjected to both LD and DD. We report that a reduced subset of genes was rhythmically expressed in vitro compared to those previously published in vivo, and that gene expression rhythms were lower in amplitude, although the functional distribution of the rhythmic transcriptome was largely similar. We also investigated the effects of 6-hour pulses of light or of norepinephrine on gene expression in free-running cultures during both subjective day and night. As expected, both light and norepinephrine inhibited melatonin production; however, the two treatments differentially enhanced or suppressed specific sets of genes in a fashion that was dependent upon time of day.
  
  
  CONCLUSIONS
  Our combined approach of utilizing a temporal, photic and pharmacological microarray experiment allowed us to identify novel genes linking clock input to clock function within the pineal. We identified approximately 30 rhythmic, light-responsive, NE-insensitive genes with no previously known clock function, which may play a role in circadian regulation of the pineal. These are candidates for future functional genomics experiments to elucidate their potential role in circadian physiology. Further, we hypothesize that the pineal circadian transcriptome is reduced but functionally conserved in vitro, and supports an endogenous role for the pineal in regulating local rhythms in metabolism, immune function, and other conserved pathways.
authors.affiliation: Department of Biology, Texas A&M University, College Station, TX 77843-3258, USA. skaraganis@bio.tamu.edu
authors.forename: Stephen P
authors.initials: SP
authors.lastname: Karaganis
authors.forename: Vinod
authors.initials: V
authors.lastname: Kumar
authors.forename: Phillip D
authors.initials: PD
authors.lastname: Beremand
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Bailey
authors.forename: Terry L
authors.initials: TL
authors.lastname: Thomas
authors.forename: Vincent M
authors.initials: VM
authors.lastname: Cassone
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002940:Circadian Rhythm / Q000187:drug effects / Q000235:genetics* / Q000502:physiology / Q000528:radiation effects; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D020869:Gene Expression Profiling; D023281:Genomics; D066298:In Vitro Techniques; D008550:Melatonin / Q000378:metabolism; D009638:Norepinephrine / Q000494:pharmacology; D020411:Oligonucleotide Array Sequence Analysis; D010775:Photic Stimulation; D017440:Photoperiod; D010870:Pineal Gland / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D008550:Melatonin; D009638:Norepinephrine