title: Factor VLeiden and prothrombin G20210A gene polymorphisms in patients with coronary artery disease.
abstract: OBJECTIVE
  The precise molecular mechanisms culminating in coronary artery disease (CAD) are not well understood, despite a wealth of knowledge on predisposing risk factors and pathomechanisms. CAD and myocardial infarction (MI) are complex genetic diseases; neither the environment alone, nor a single gene, cause disease, rather, a mix of environmental and genetic factors lead to atherosclerosis of the coronary arteries.
  
  
  METHODS
  In the present study, our aim was to investigate the roles of prothrombin G20210A mutation and Factor VLeiden mutation in atherosclerotic coronary artery disease. 287 subjects (106 control subjects, who were angiographically normal, and 181 angiographically documented coronary atherosclerotic patients who exhibited coronary artery narrowing to a degree of >or=50%) were included in this study. The mutations were assessed with LightCycler Real-Time PCR mutation detection kits (Roche Diagnostics, GmbH, Germany).
  
  
  RESULTS
  6.6% of control subjects, and 6.1% of patients with (50% coronary artery narrowing were determined to have the Factor VLeiden heterozygote mutation. 6.6% of control subjects had the Prothrombin G20210A heterozygote mutation, while 7.7% of patients with (50% coronary artery narrowing had this mutation. The OR for Factor VLeiden was 1.52 (CI: 0.240-9.602) and for Prothrombin G20210A mutation, the OR was 1.415 (CI: 0.287-6.962).
  
  
  CONCLUSIONS
  Although both the heterozygote Factor VLeiden and Prothrombin gene mutations were more frequent in patients with CAD than in control subjects, there was no statistical relationship found to exist between coronary artery disease and the Factor VLeiden and Prothrombin G20210A mutations.
authors.affiliation: Department of Biochemistry, Mersin University, Medical Faculty, Mersin, Turkey.
authors.forename: Bahadir
authors.initials: B
authors.lastname: Ercan
authors.forename: Lülüfer
authors.initials: L
authors.lastname: Tamer
authors.forename: Nehir
authors.initials: N
authors.lastname: Sucu
authors.forename: Hasan
authors.initials: H
authors.lastname: Pekdemir
authors.forename: Ahmet
authors.initials: A
authors.lastname: Camsari
authors.forename: Uğur
authors.initials: U
authors.lastname: Atik
mesh_terms: D000368:Aged; D003324:Coronary Artery Disease / Q000235:genetics*; D005165:Factor V / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D011516:Prothrombin / Q000235:genetics*
chemical_list: C095381:factor V Leiden; D005165:Factor V; D011516:Prothrombin