Sentinel lymph node detection ex vivo using ultrasound-modulated optical tomography.
We apply ultrasound-modulated optical tomography (UOT) to image ex-vivo methylene-blue-dyed sentinel lymph nodes embedded in 3.2-cm-thick chicken breast tissues. The UOT system is implemented for the first time using ring-shaped light illumination, intense acoustic bursts, and charge-coupled device (CCD) camera-based speckle contrast detection. Since the system is noninvasive, nonionizing, portable, relatively cost effective, and easy to combine with photoacoustic imaging and single element ultrasonic pulse-echo imaging, UOT can potentially be a good imaging modality for the detection of sentinel lymph nodes in breast cancer staging in vivo.
Kim
Chulhong
C
Washington University in Saint Louis, Optical Imaging Laboratory, Department of Biomedical Engineering, Campus Box 1097, One Brookings Drive, Saint Louis, Missouri 63130-4899, USA.
Song
Kwang Hyun
KH
Wang
Lihong V
LV
D000818:Animals; D001940:Breast / Q000166:cytology*; D002645:Chickens; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers; D008198:Lymph Nodes / Q000166:cytology* / Q000000981:diagnostic imaging*; D033401:Microscopy, Acoustic / Q000295:instrumentation* / Q000379:methods; D021701:Sentinel Lymph Node Biopsy / Q000295:instrumentation / Q000379:methods; D041622:Tomography, Optical / Q000295:instrumentation* / Q000379:methods; D016217:Ultrasonography, Mammary / Q000295:instrumentation* / Q000379:methods