title: Changes in the status of nucleolus during long-term culturing of human HeLa cells.
abstract: Changes in the immunocytochemical status of the nucleoli during long-term (6-8 months) in vitro culturing of HeLa (carcinoma of the cervix uteri) cells were described using new A3 monoclonal antibodies selectively reacting with human cell nucleoli. The appearance of cells with abnormal location of A3 antigen was paralleled by a significant increase of culture sensitivity to some external factors (protein synthesis inhibition and oxidative stress). The data indicate that location of one of the nucleolar antigens is an indicator of the qualitative status of HeLa cells in the culture.
authors.affiliation: M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Organic Biochemistry, Russian Academy of Sciences, Moscow.
authors.forename: A A
authors.initials: AA
authors.lastname: Grigoryev
authors.forename: O O
authors.initials: OO
authors.lastname: Zharskaya
authors.forename: T I
authors.initials: TI
authors.lastname: Bulycheva
authors.forename: O V
authors.initials: OV
authors.lastname: Zatsepina
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D000941:Antigens / Q000378:metabolism; D018929:Cell Culture Techniques; D002466:Cell Nucleolus / Q000378:metabolism* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D013997:Time Factors
chemical_list: D000911:Antibodies, Monoclonal; D000941:Antigens