title: Bovine endometrial legumain and TIMP-2 regulation in response to presence of a conceptus.
abstract: The precise mechanism of placentation in the bovine species where a restricted trophoblast invasion occurs to form the synepitheliochorial placenta is not fully understood. This study initially investigated the conceptus-maternal interactions in the peri-attachment period by comparing the proteins present at Days 16 and 18 in uterine luminal fluid (ULF) of pregnant with nonpregnant cows using 2-D gel electrophoresis. Nine protein spots were identified that were present in greater amounts in pregnant compared to nonpregnant ULF: carbonic anhydrase, ezrin, heat shock protein 70, isocitrate dehydrogenase, nucleoside diphosphate kinase, peroxiredoxin 1, purine nucleoside phosphorylase, thioredoxin and triosephosphate isomerase and four proteins that were less abundant in ULF from the gravid compared to the nongravid horns or nonpregnant uteri: cystatin E/M, legumain, retinol-binding protein (RBP) and tissue inhibitor of matrix metalloproteinase 2 (TIMP-2). Successful placentation requires the remodelling of the endometrial surface therefore uterine mRNA and protein expression of legumain, a protease activator, and TIMP-2, a protease inhibitor, was examined in detail during the oestrous cycle and from Days 13 to 31 of pregnancy. Both mRNAs were up-regulated in the endometrium during the luteal phase of the oestrous cycle and during early pregnancy. Although legumain and TIMP-2 mRNA expression levels were similar between uterine horns at the same day of pregnancy, the amount of protein differed between gravid and nongravid horns possibly modulated by interferon-tau or by other factors produced by the conceptus. These events at the conceptus-maternal interface may provide localised control of protease activity necessary for controlling trophoblast invasion of the endometrium.
authors.affiliation: AgResearch, Reproductive Technologies Section, Ruakura Research Centre, Hamilton, New Zealand. anita.ledgard@agresearch.co.nz
authors.forename: A M
authors.initials: AM
authors.lastname: Ledgard
authors.forename: R S-F
authors.initials: RS
authors.lastname: Lee
authors.forename: A J
authors.initials: AJ
authors.lastname: Peterson
mesh_terms: D000818:Animals; D002417:Cattle; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D004622:Embryo, Mammalian / Q000201:enzymology*; D004717:Endometrium / Q000201:enzymology*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics; D019716:Tissue Inhibitor of Metalloproteinase-2 / Q000235:genetics / Q000378:metabolism*; D000099240:Asparaginyl Endopeptidase
chemical_list: D003546:Cysteine Endopeptidases; D012333:RNA, Messenger; D019716:Tissue Inhibitor of Metalloproteinase-2; D000099240:Asparaginyl Endopeptidase