No gene copy number changes in Dupuytren's contracture by array comparative genomic hybridization.
Dupuytren's contracture (DC), a benign disease of unknown origin, is characterized by abnormal fibroblast proliferation and matrix deposition within the palmar and plantar faciae, causing contracture of the digits. Conventional cytogenetic studies of cultured fibroblast cells from DC nodules have revealed nonrecurrent, but usually normal, clonal (mainly +7, +8, and -Y, plus structural changes) and sporadic (nonclonal) numerical/structural rearrangements. No unique cytogenetic features of DC are known so far. We used 44K oligonucleotide-based array comparative genomic hybridization to obtain a wide pattern of chromosomal imbalances in 18 patients with DC. The genome-wide analysis revealed no changes of DNA copy number sequences. Accordingly, gene amplifications or deletions are apparently not involved in the progression of abnormal fibroblast proliferation and matrix deposition that lead to DC.
Kaur
Sippy
S
Department of Pathology, Haartman Institute and HUSLAB, University of Helsinki and University Central Hospital, POB 21 (Haartmaninkatu 3), Helsinki 00014, Finland.
Forsman
Minna
M
Ryhänen
Jorma
J
Knuutila
Sakari
S
Larramendy
Marcelo L
ML
D000368:Aged; D000369:Aged, 80 and over; D004387:Dupuytren Contracture / Q000235:genetics*; D005260:Female; D018628:Gene Dosage; D020869:Gene Expression Profiling; D015894:Genome, Human; D006801:Humans; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*