Pre-mRNA splicing: a complex picture in higher definition.
Intron excision from pre-mRNAs of higher eukaryotes requires a transition from splice-site recognition across short exons to organization of the spliceosome across long introns. Recently, insight into this transition has been provided and, in addition, it has been shown that an alternative splicing factor, the polypyrimidine-tract-binding protein, can exert its control on splice-site choice by blocking this key step in the assembly of the splicing machinery.
Schellenberg
Matthew J
MJ
Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
Ritchie
Dustin B
DB
MacMillan
Andrew M
AM
D000818:Animals; D005057:Eukaryotic Cells / Q000378:metabolism*; D006801:Humans; D007438:Introns / Q000502:physiology; D038941:Polypyrimidine Tract-Binding Protein / Q000378:metabolism*; D012322:RNA Precursors / Q000378:metabolism*; D022821:RNA Splice Sites / Q000502:physiology*; D012326:RNA Splicing / Q000502:physiology*; D017381:Spliceosomes / Q000378:metabolism*
D012322:RNA Precursors; D022821:RNA Splice Sites; D038941:Polypyrimidine Tract-Binding Protein