Inhibitory effects of Thai plants beta-glycosides on Trichomonas vaginalis.
Trichomoniasis is now an important health problem in developing countries. Although metronidazole has so far been widely used to treat this disease, the prevalence of metronidazole-resistant protozoa and unpleasant adverse effects have been found. In this study, natural products purified from Thai plants were, therefore, investigated for their effectiveness against Trichomonas vaginalis. The minimal inhibitory concentrations for all beta-glycosides against Trichomonas vaginalis at 24 h were in a range of 6.25-12.5 microM. In addition, torvoside A and H were found to be more potent than their corresponding aglycones, deglucosylated torvoside A and H, while other beta-glycosides were generally as active as their corresponding aglycones. The cytotoxicity of these compounds was also determined. Except for dalcochinin, none of the tested compounds showed cytotoxicity against Vero and cancer cell lines (KB and MCF-7), having IC(50) values greater than 50 microg/ml. In conclusion, beta-glycosides and several aglycones showed selective inhibition against Trichomonas vaginalis without harmful effect to mammalian cells.
Arthan
Dumrongkiet
D
Department of Tropical Nutrition and Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok, 10400, Thailand.
Sithiprom
Somphong
S
Thima
Kanthinich
K
Limmatvatirat
Chutima
C
Chavalitshewinkoon-Petmitr
Porntip
P
Svasti
Jisnuson
J
D000818:Animals; D000994:Antitrichomonal Agents / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor / Q000187:drug effects; D002522:Chlorocebus aethiops; D006027:Glycosides / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D020128:Inhibitory Concentration 50; D021261:Parasitic Sensitivity Tests; D010946:Plants, Medicinal / Q000737:chemistry*; D013785:Thailand; D014246:Trichomonas vaginalis / Q000187:drug effects*; D014709:Vero Cells / Q000187:drug effects
D000994:Antitrichomonal Agents; D006027:Glycosides