Antigen-specific T-cell induction by vaccination with a recombinant Sendai virus vector even in the presence of vector-specific neutralizing antibodies in rhesus macaques.
Recombinant viral vectors are promising vaccine tools for eliciting potent cellular immune responses against immunodeficiency virus infection, but pre-existing anti-vector antibodies can be an obstacle to their clinical use in humans. We have previously vaccinated rhesus macaques with a recombinant Sendai virus (SeV) vector twice at an interval of more than 1 year and have shown efficient antigen-specific T-cell induction by the second as well as the first vaccination. Here, we have established the method for measurement of SeV-specific neutralizing titers and have found efficient SeV-specific neutralizing antibody responses just before the second SeV vaccination in these macaques. This suggests the feasibility of inducing antigen-specific T-cell responses by SeV vaccination even in the host with pre-existing anti-SeV neutralizing antibodies.
Moriya
Chikaya
C
International Research Center for Infectious Diseases, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Horiba
Satoshi
S
Inoue
Makoto
M
Iida
Akihiro
A
Hara
Hiroto
H
Shu
Tsugumine
T
Hasegawa
Mamoru
M
Matano
Tetsuro
T
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D000941:Antigens / Q000276:immunology; D005822:Genetic Vectors / Q000235:genetics / Q000276:immunology*; D008213:Lymphocyte Activation; D008253:Macaca mulatta; D009500:Neutralization Tests / Q000379:methods*; D029082:Sendai virus / Q000235:genetics / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014611:Vaccination
D000914:Antibodies, Viral; D000941:Antigens