[Effect of 5-HT and postsynaptic 5-HT1 A on the mood and recogniztion of the repeated restraint stress in rats].
OBJECTIVE
To explore the role of 5-HT and postsynaptic 5-HT1A receptors in the stress adaptation.


METHODS
p-PCA was used to deplete the 5-HT in rats. The 5-HT1A agonist 8-OH-DPAT and antagonist WAY100635 were used to determine the effect of postsynaptic 5-HT1A receptors on the ratso behaviors in the Elevated Plus-Maze test, the Forced Swimming test, and the Morris Water Maze test.


RESULTS
Compared with the intact rats, the 5-HT depleted rats showed more seriously anxious behaviors in the Elevated Plus-Maze test and more obvious learned helplessness in the Forced Swimming test. After having been stressed the 5-HT depleted rats showed significantly impaired learning and memory compared with the intact rats according to Morris Water Maze test. Activation of postsynaptic 5-HT1A receptors by 8-OH-DPAT in the 5-HT depleted rats or the 5-HT depleted stress rats significantly decreased the symptoms of anxiety and learned helplessness behaviors which were prevented by the treatment of WAY100635. The 8-OH-DPAT and WAY100635 had no obvious effect on the 5-HT depletion or 5-HT depleted stress rats in the Morris Water Maze test.


CONCLUSIONS
Deficiency of 5-HT in rats may suppress its ability to stress adaptation. Activation of post-synaptic 5-HT1A receptors can attenuate the anxiety and depressive behavior symptoms, and facilitate rats to adapt stress.
Zhou
Jian-song
JS
Mental Health Institute, Second Xiangya Hospital, Central South University, Changsha, China.
Li
Lingjiang
L
Cao
Xia
X
Zhang
Xiang-hui
XH
Li
Wei-hui
WH
Li
Ze-xuan
ZX
D000339:Affect / Q000502:physiology; D000818:Animals; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D044282:Receptor, Serotonin, 5-HT1A / Q000502:physiology*; D021641:Recognition, Psychology / Q000502:physiology*; D012149:Restraint, Physical; D012701:Serotonin / Q000502:physiology*; D013315:Stress, Psychological / Q000378:metabolism* / Q000523:psychology*; D013574:Synaptosomes / Q000737:chemistry
D044282:Receptor, Serotonin, 5-HT1A; D012701:Serotonin