Fate mapping of neural crest cells during eye development using a protein 0 promoter-driven transgenic technique.
OBJECTIVE
To map neural crest cell fate during eye development.


METHODS
Neural crest cells were tracked in developing mouse eyes using a transgene expressing Cre recombinase controlled by the Protein 0 promoter and a Rosa26 Cre-responsive reporter gene that produced beta-galactosidase after Cre-mediated recombination.


RESULTS
beta-galactosidase-positive cells were detected in the periocular segment on embryonic day (E) 9.5. Several neural crest cell-derived tissues including corneal stroma, corneal endothelium, iridocorneal angle, ciliary body, primary vitreous and eyelid were strongly stained on E13.5-E18.5. The staining decreased in the corneal stroma after birth, but persisted in the presumptive iridocorneal angle.


CONCLUSIONS
Protein 0-Cre transgenic mice offer a conditional knock-out strategy to investigate anterior eye segment differentiation.
Iwao
Keiichiro
K
Department of Ophthalmology and Visual Science, Kumamoto University Graduate School of Medical Sciences, 1-1-1, Honjo, 860-8556, Kumamoto, Japan.
Inatani
Masaru
M
Okinami
Satoshi
S
Tanihara
Hidenobu
H
D000818:Animals; D005123:Eye / Q000166:cytology / Q000196:embryology*; D005260:Female; D005786:Gene Expression Regulation; D017930:Genes, Reporter; D019426:Integrases / Q000235:genetics; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D018993:Myelin P0 Protein / Q000235:genetics*; D009432:Neural Crest / Q000166:cytology* / Q000196:embryology*; D011401:Promoter Regions, Genetic; D011506:Proteins; D022661:RNA, Untranslated; D019076:Transgenes; D001616:beta-Galactosidase / Q000378:metabolism
C110853:Gt(ROSA)26Sor non-coding RNA, mouse; D018993:Myelin P0 Protein; D011506:Proteins; D022661:RNA, Untranslated; C045073:Cre recombinase; D019426:Integrases; D001616:beta-Galactosidase