title: Inhibition of in vitro amplification of targeted DNA fragment and activity of exonuclease I by a fullerene-oligonucleotide conjugate.
abstract: Novel biological activities of a fullerene-oligonucleotide conjugate (FONC) were examined in the present report. A new FONC containing a complementary sequence to a specific region of the beta-actin cDNA was synthesized. We found that the FONC at a low concentration (10(-6)M) could specifically inhibit PCR-amplification of the beta-actin cDNA by inhibiting the activities of both Taq DNA polymerase and the cDNA template. This inhibition appeared to be mediated by reactive oxygen species (ROS). The FONC also exhibited the ability of antagonizing the enzymatic activity of an engineered nuclease, exonuclease I (Exo I). Our data provided some valuable evidences on the prospective use of FONC in the antisense strategy for gene silencing.
authors.affiliation: School of Life Science and Technology, Beijing Institute of Technology, 5 Zhongguancun South Street, Beijing 100081, China. xlyang@bit.edu.cn
authors.forename: Xinlin
authors.initials: X
authors.lastname: Yang
authors.forename: Xianmei
authors.initials: X
authors.lastname: Meng
authors.forename: Bo
authors.initials: B
authors.lastname: Li
authors.forename: Zhe
authors.initials: Z
authors.lastname: Chen
authors.forename: Dongxu
authors.initials: D
authors.lastname: Zhao
authors.forename: Xin
authors.initials: X
authors.lastname: Tan
authors.forename: Qun
authors.initials: Q
authors.lastname: Yu
mesh_terms: D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000138:chemical synthesis / Q000494:pharmacology; D005090:Exodeoxyribonucleases / Q000037:antagonists & inhibitors* / Q000378:metabolism; D037741:Fullerenes / Q000737:chemistry / Q000494:pharmacology*; D005784:Gene Amplification / Q000187:drug effects*; D020868:Gene Silencing; D006367:HeLa Cells; D006801:Humans; D008954:Models, Biological; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis / Q000494:pharmacology*; D009841:Oligonucleotides / Q000737:chemistry / Q000494:pharmacology*; D016133:Polymerase Chain Reaction / Q000379:methods; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D037741:Fullerenes; D009838:Oligodeoxyribonucleotides; D009841:Oligonucleotides; D017382:Reactive Oxygen Species; D004247:DNA; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I