Modulatory effects of acetazolomide and dexamethasone on temozolomide-mediated apoptosis in human glioblastoma T98G and U87MG cells.
Acetazolomide (ACZ) and dexamethasone (DXM) alleviate vasogenic edema and inflammation in glioblastoma patients. Temozolomide (TMZ) is used for treating glioblastoma. We compared modulatory effects of ACZ and DXM on TMZ mediated apoptosis in human glioblastoma T98G and U87MG cells. Cells were treated with drug(s) for 6 h and then left in drug-free medium for 48 h. Although ACZ or DXM alone did not induce apoptosis, TMZ alone induced significant amount of apoptosis. Interestingly, ACZ pretreatment enhanced apoptosis while DXM pretreatment decreased apoptosis. These results suggest that combination chemotherapy with ACZ and TMZ may control inflammation and enhance apoptosis in glioblastoma.
Das
Arabinda
A
Department of Neurosciences (Neurology), Medical University of South Carolina, Charleston, South Carolina 29425, USA.
Banik
Naren L
NL
Ray
Swapan K
SK
D000086:Acetazolamide / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D051398:Aquaporin 1 / Q000096:biosynthesis / Q000235:genetics; D001929:Brain Edema / Q000517:prevention & control; D001932:Brain Neoplasms / Q000473:pathology*; D002154:Calpain / Q000378:metabolism; D002256:Carbonic Anhydrases / Q000096:biosynthesis / Q000235:genetics; D020169:Caspases / Q000378:metabolism; D045744:Cell Line, Tumor / Q000187:drug effects; D003606:Dacarbazine / Q000031:analogs & derivatives* / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology*; D004347:Drug Interactions; D004354:Drug Screening Assays, Antitumor; D005909:Glioblastoma / Q000473:pathology*; D006801:Humans; D007249:Inflammation / Q000517:prevention & control; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology; D016328:NF-kappa B / Q000096:biosynthesis / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D000077204:Temozolomide; D042461:Vascular Endothelial Growth Factor A / Q000096:biosynthesis / Q000235:genetics
D016328:NF-kappa B; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; D051398:Aquaporin 1; D003606:Dacarbazine; D003907:Dexamethasone; D002154:Calpain; D020169:Caspases; D002256:Carbonic Anhydrases; D000086:Acetazolamide; D000077204:Temozolomide