title: Hypolipidaemic effects of fenofibrate and fasting in the herbivorous grass carp ( Ctenopharyngodon idella) fed a high-fat diet.
abstract: We investigated whether the hypolipidaemic effect of fenofibrate and fasting observed in most omnivorous mammals may also apply to herbivorous fish. Grass carp (Ctenopharyngodon idella) fed a high-fat (8 %) diet exhibited a marked increase in blood lipids and body fat after 6 weeks. They were then treated with fenofibrate (100 mg/kg body weight) in the same high-fat diet for 2 weeks, followed by fasting for 1 week. Plasma lipid concentration, body fat amount, fatty acid composition, plasma thiobarbituric acid-reactive substances and some parameters related to hepatic fatty acid oxidation were measured, and liver samples were stained for histological examination. Fenofibrate treatment decreased TAG and cholesterol concentrations in plasma, total lipids of the whole body and liver, and EPA and DHA contents in tissues. Further, a mobilisation of mesenteric fat concomitant with an increase in hepatic peroxisomal fatty acid oxidation and lipid peroxidation was observed. Compared with fenofibrate treatment, fasting decreased body weight and plasma TAG, but not plasma cholesterol. It also reduced the fat content of the whole body and increased the EPA and DHA contents in the liver and other tissues. Fatty acid oxidation was stimulated by fasting in mitochondria, but not in peroxisomes. These data suggest that fenofibrate and fasting regulate the lipid metabolism in grass carp through different metabolic pathways. The grass carp is moderately responsive to a fibrate derivative in comparison with the well-known excess responsiveness of the rat model, and so it could be used for the study of lipid abnormalities as a herbivorous model.
authors.affiliation: Institute of Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, 135 Xin'gang Xi Road, 510275 Guangzhou, China. edls@mail.sysu.edu.cn
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: Du
authors.forename: Pierre
authors.initials: P
authors.lastname: Clouet
authors.forename: Pascal
authors.initials: P
authors.lastname: Degrace
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Zheng
authors.forename: Livar
authors.initials: L
authors.lastname: Frøyland
authors.forename: Li-Xia
authors.initials: LX
authors.lastname: Tian
authors.forename: Yong-Jian
authors.initials: YJ
authors.lastname: Liu
mesh_terms: D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D002347:Carps; D003131:Combined Modality Therapy; D004041:Dietary Fats / Q000008:administration & dosage; D005215:Fasting; D005227:Fatty Acids / Q000378:metabolism; D011345:Fenofibrate / Q000627:therapeutic use*; D005393:Fish Diseases / Q000209:etiology / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D006949:Hyperlipidemias / Q000378:metabolism / Q000473:pathology / Q000628:therapy* / Q000662:veterinary*; D000960:Hypolipidemic Agents / Q000627:therapeutic use*; D050356:Lipid Metabolism / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000378:metabolism / Q000473:pathology; D010084:Oxidation-Reduction / Q000187:drug effects; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D000960:Hypolipidemic Agents; D017392:Thiobarbituric Acid Reactive Substances; D011345:Fenofibrate