title: Synthesis and transfection activity of new cationic phosphoramidate lipids: high efficiency of an imidazolium derivative.
abstract: In an effort to enhance the gene-transfer efficiencies of cationic lipids and to decrease their toxicities, a series of new phosphoramidate lipids with chemical similarity to cell membrane phospholipids was synthesised. These lipids contained various cationic headgroups, such as arginine methyl ester, lysine methyl ester, homoarginine methyl ester, ethylenediamine, diaminopropane, guanidinium and imidazolium. Their transfection abilities, either alone or with the co-lipid DOPE, were evaluated in HEK293-T7 cells. We found that imidazolium lipophosphoramidate 7 a/DOPE lipoplexes gave the most efficient transfection with low toxicity (15 %). The luciferase activity was 100 times higher than that obtained with DOTAP/DOPE lipoplexes. The size, zeta potential, pDNA-liposome interactions and cellular uptakes of the lipoplexes were determined. No definitive correlation between the zeta potential values and the transfection efficiencies could be established, but the uptake of lipoplexes by the cells was correlated with their final transfection efficiencies. Our results show that imidazolium phosphoramidate lipids constitute a potential new class of cationic lipids for gene transfer.
authors.affiliation: CEMCA, UMR CNRS 6521, Faculté des Sciences et Techniques, Université de Bretagne Occidentale, 6 Avenue Le Gorgeu, 29238 Brest, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Mével
authors.forename: Gilles
authors.initials: G
authors.lastname: Breuzard
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Yaouanc
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Clément
authors.forename: Pierre
authors.initials: P
authors.lastname: Lehn
authors.forename: Chantal
authors.initials: C
authors.lastname: Pichon
authors.forename: Paul-Alain
authors.initials: PA
authors.lastname: Jaffrès
authors.forename: Patrick
authors.initials: P
authors.lastname: Midoux
mesh_terms: D000596:Amino Acids / Q000737:chemistry; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism / Q000633:toxicity; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000378:metabolism; D004952:Esters / Q000737:chemistry; D018773:Genes, erbB-1 / Q000235:genetics; D019791:Guanidine / Q000737:chemistry; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008156:Luciferases / Q000378:metabolism; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D010957:Plasmids / Q000378:metabolism; D014162:Transfection / Q000379:methods*; D019076:Transgenes
chemical_list: C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D000596:Amino Acids; D004952:Esters; D007093:Imidazoles; D008081:Liposomes; D010714:Phosphatidylethanolamines; D010743:Phospholipids; D004247:DNA; D008156:Luciferases; D019791:Guanidine