Characterization of the novel gene BjDREB1B encoding a DRE-binding transcription factor from Brassica juncea L.
A novel DREB (dehydration responsive element binding protein) gene, designated BjDREB1B, was isolated from Brassica juncea L. BjDREB1B contains a conserved EREBP/AP2 domain and was classified into the A-1 subgroup of the DREB subfamily based on phylogenetic tree analysis. RT-PCR showed that BjDREB1B was induced by abiotic stresses and exogenous phytohormones, such as drought, salt, low temperature, heavy metals, abscisic acid, and salicylic acid. Gel shift assay revealed that BjDREB1B specifically bound to the DRE element in vitro. Yeast one-hybrid assay showed that full-length BjDREB1B or its C-terminal region functioned effectively as a trans-activator. Furthermore, overexpression of BjDREB1B in tobacco up-regulated the expression of NtERD10B, and BjDREB1B transgenic plants accumulated higher levels of proline than control plants under normal and saline conditions, together showing that BjDREB1B plays important roles in improving plant tolerance to drought and salinity.
Cong
Lin
L
College of Life Science, Graduate University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China.
Chai
Tuan-Yao
TY
Zhang
Yu-Xiu
YX
D000222:Adaptation, Physiological / Q000235:genetics*; D024202:Electrophoretic Mobility Shift Assay; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D009149:Mustard Plant / Q000235:genetics* / Q000378:metabolism*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D017434:Protein Structure, Tertiary; D020218:Response Elements; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012492:Salts / Q000378:metabolism; D013696:Temperature; D014026:Nicotiana / Q000235:genetics; D015534:Trans-Activators / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020798:Two-Hybrid System Techniques; D014867:Water / Q000737:chemistry
D010940:Plant Proteins; D012492:Salts; D015534:Trans-Activators; D014867:Water