title: Heterologous expression of functionally active enterolysin A, class III bacteriocin from Enterococcus faecalis, in Escherichia coli.
abstract: The heterologous expression of enterolysin A (EnlA), heat-labile class III bacteriocin from Enterococcus faecalis II/1 with anti-listerial activity, was studied in Escherichia coli. The PCR amplified products of enterolysin A structural gene, N-terminal part of EnlA with endopeptidase-like activity and C-terminal part of EnlA similar to a lysis gene of bacteriophage, were cloned in prelinearized pQE-30UA expression vector. The expression of EnlA structural gene led to the synthesis and secretion of functional-active His-tagged enterolysin A protein, which was purified to homogeneity using His-Select Cartridge and was shown to be fully active against the indicator strain. The expression of N-terminal or C-terminal part of EnlA and deletion of last 58 amino acids from C-terminal domain of EnlA led to the synthesis of biologically non-active proteins.
authors.affiliation: Department of Microbial Genetics, Institute of Animal Physiology, Slovak Academy of Sciences, Soltésovej 4-6, 04001 Kosice, Slovak Republic.
authors.forename: Katarína
authors.initials: K
authors.lastname: Nigutová
authors.forename: Lenka
authors.initials: L
authors.lastname: Serencová
authors.forename: Mária
authors.initials: M
authors.lastname: Piknová
authors.forename: Peter
authors.initials: P
authors.lastname: Javorský
authors.forename: Peter
authors.initials: P
authors.lastname: Pristas
mesh_terms: D001430:Bacteriocins / Q000302:isolation & purification / Q000378:metabolism*; D003001:Cloning, Molecular; D013293:Enterococcus faecalis / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism*
chemical_list: D001430:Bacteriocins; C522987:enterolysin A, Enterococcus faecalis