One-way biohydrogen transfer for oxidation of sec-alcohols.
Quasi-irreversible oxidation of sec-alcohols was achieved via biocatalytic hydrogen transfer reactions using alcohol dehydrogenases employing selected ketones as hydrogen acceptors, which can only be reduced but not oxidized. Thus, only 1 equiv of oxidant was required instead of a large excess. For the oxidation of both isomers of methylcarbinols a single nonstereoselective short-chain dehydrogenase/reductase from Sphingobium yanoikuyae was identified and overexpressed in E. coli.
Lavandera
Iván
I
Research Centre Applied Biocatalysis c/o Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Graz, Austria.
Kern
Alexander
A
Resch
Verena
V
Ferreira-Silva
Bianca
B
Glieder
Anton
A
Fabian
Walter M F
WM
de Wildeman
Stefaan
S
Kroutil
Wolfgang
W
D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism; D000438:Alcohols / Q000737:chemistry*; D020561:Alphaproteobacteria / Q000201:enzymology; D002384:Catalysis; D006859:Hydrogen / Q000737:chemistry*; D007659:Ketones / Q000737:chemistry; D007700:Kinetics; D010084:Oxidation-Reduction; D013997:Time Factors
D000438:Alcohols; D007659:Ketones; D006859:Hydrogen; D000426:Alcohol Dehydrogenase