title: Accumulation of 99mTc-ciprofloxacin in Staphylococcus aureus and Pseudomonas aeruginosa.
abstract: The use of radiopharmaceuticals for the diagnosis of infection is increasing due to their ability to distinguish between septic and aseptic inflammation. The aim of this study was to analyze the intracellular accumulation of technetium-99m-ciprofloxacin in strains of Staphylococcus aureus and Pseudomonas aeruginosa harboring an overexpression of NorA and MexAB-OprM, respectively.
authors.affiliation: Department of Infectious Diseases, Hospital Clinic, IDIBAPS, School of Medicine, University of Barcelona, Barcelona, Spain. jmsierra@clinic.ub.es
authors.forename: J M
authors.initials: JM
authors.lastname: Sierra
authors.forename: D
authors.initials: D
authors.lastname: Rodriguez-Puig
authors.forename: A
authors.initials: A
authors.lastname: Soriano
authors.forename: J
authors.initials: J
authors.lastname: Mensa
authors.forename: C
authors.initials: C
authors.lastname: Piera
authors.forename: J
authors.initials: J
authors.lastname: Vila
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002939:Ciprofloxacin / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D015870:Gene Expression; D005798:Genes, Bacterial; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics*; D011552:Pseudomonas Infections / Q000000981:diagnostic imaging / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000378:metabolism*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics*; D013203:Staphylococcal Infections / Q000000981:diagnostic imaging / Q000382:microbiology; D013211:Staphylococcus aureus / Q000235:genetics / Q000378:metabolism*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002939:Ciprofloxacin; D026901:Membrane Transport Proteins; D027425:ATP-Binding Cassette, Sub-Family C Proteins; D015609:Organotechnetium Compounds; D019275:Radiopharmaceuticals; C118598:MexA protein, Pseudomonas aeruginosa; C118597:MexB protein, Pseudomonas aeruginosa; C402263:OprM protein, Pseudomonas aeruginosa; C431804:Technetium Tc 99m ciprofloxacin; C075040:NorA protein, Staphylococcus