title: Cytotoxic necrotizing factor 1 (CNF1) induces an inflammatory response in the urinary tract in vitro but not in vivo.
abstract: Cytotoxic necrotizing factor 1 (CNF1) is a well-defined virulence factor of uropathogenic Escherichia coli. We studied the role of CNF1 in uroepithelial cells as well as in children and adults with sporadic and recurrent UTI. Our study suggests that CNF1 may promote bacterial attachment and invasion and can induce an inflammatory response in the urinary tract in vitro but that its role in vivo is possibly minor in comparison with other virulence factors of the uropathogenic E. coli.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Division of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Olof
authors.initials: O
authors.lastname: Hertting
authors.forename: Milan
authors.initials: M
authors.lastname: Chromek
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Slamová
authors.forename: Lavinia
authors.initials: L
authors.lastname: Kádas
authors.forename: Maja
authors.initials: M
authors.lastname: Söderkvist
authors.forename: Inga
authors.initials: I
authors.lastname: Vainumäe
authors.forename: Tiina
authors.initials: T
authors.lastname: Tallvik
authors.forename: Stefan H
authors.initials: SH
authors.lastname: Jacobson
authors.forename: Annelie
authors.initials: A
authors.lastname: Brauner
mesh_terms: D001427:Bacterial Toxins / Q000235:genetics; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000472:pathogenicity*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000502:physiology*; D006801:Humans; D009154:Mutation; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000378:metabolism; D014551:Urinary Tract / Q000382:microbiology*; D014552:Urinary Tract Infections / Q000382:microbiology; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*
chemical_list: D001427:Bacterial Toxins; D029968:Escherichia coli Proteins; D019715:Tissue Inhibitor of Metalloproteinase-1; D037521:Virulence Factors; C060296:cytotoxic necrotizing factor type 1