title: Activation of Akt/protein kinase B and extracellular signal-regulated kinase in rats with acute experimental testicular torsion.
abstract: The Akt/protein kinase B (PKB) and extracellular signal-regulated kinase (ERK) pathways are involved in cell survival. This study examined the temporal profiles and localization of Akt/PKB and ERK1/2 activation in rat testis after ischemia/reperfusion (I/R). Testicular tissue was collected from normal control rats and rats exposed to reperfusion for 6, 24, and 48 hr after ischemic injury; the tissues were analyzed via Western blotting and immunohistochemistry. Western blot analysis showed that the levels of phosphorylated Akt/PKB (pAkt/PKB) and ERK1/2 (pERK1/2) increased significantly during the first 6-24 hr of reperfusion after ischemia. However, both of these activated proteins were decreased slightly at 48 hr after reperfusion. Immunohistochemically, low levels of pAkt/PKB expression were observed in Sertoli cells from the normal control. After I/R, pAkt/PKB expression increased mainly in the adluminal portion of the Sertoli cells, as well as in spermatogenic cells. In addition, pERK1/2 expression was observed in Sertoli and Leydig cells in the normal control. After I/R, pERK1/2 expression increased in some surviving spermatogenic cells (mainly spermatocytes), as well as in the adluminal portion of Sertoli cells. These results suggest that both Akt/PKB and ERK1/2 are involved in the survival of testicular cells during the early phase of testicular I/R. These pathways may represent important targets for increasing cell survival in testicular injury, including testicular torsion.
authors.affiliation: Department of Veterinary Anatomy, College of Veterinary Medicine, Chonnam National University, Gwangju, South Korea. moonc@chonnam.ac.kr
authors.forename: Changjong
authors.initials: C
authors.lastname: Moon
authors.forename: Joong-sun
authors.initials: JS
authors.lastname: Kim
authors.forename: Hyosun
authors.initials: H
authors.lastname: Jang
authors.forename: Hae-june
authors.initials: HJ
authors.lastname: Lee
authors.forename: Sung-ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Seong Soo
authors.initials: SS
authors.lastname: Kang
authors.forename: Chun-Sik
authors.initials: CS
authors.lastname: Bae
authors.forename: Jong-Choon
authors.initials: JC
authors.lastname: Kim
authors.forename: Seungjoon
authors.initials: S
authors.lastname: Kim
authors.forename: Yongduk
authors.initials: Y
authors.lastname: Lee
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D008297:Male; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D012671:Seminiferous Tubules / Q000473:pathology; D013086:Spermatic Cord Torsion / Q000235:genetics / Q000378:metabolism*
chemical_list: D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases