title: Glycation of plasma lipoprotein lipid membrane and screening for lipid glycation inhibitor.
abstract: We recently reported that phosphatidylethanolamine (PE)-linked Amadori product (Amadori-PE) increased abnormally in diabetic plasma. However, the glycation mechanism of human plasma low-density lipoprotein (LDL) is still unclear. Moreover, lipid glycation inhibitors have yet to be discovered. In this study, we compared the glycation kinetics of LDL lipid and LDL protein in vitro and screened lipid glycation inhibitors. LDL-PE was converted to Amadori-PE followed by LDL protein (apoB) glycation. Pyridoxal 5'-phosphate could easily react with PE before the glucose-PE reaction, and the PE-pyridoxal 5'-phosphate adduct was detected in human red blood cells. Pyridoxal 5'-phosphate can be used in diabetes prevention.
authors.affiliation: Food and Biodynamic Chemistry Laboratory, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
authors.forename: Kiyotaka
authors.initials: K
authors.lastname: Nakagawa
authors.forename: Daigo
authors.initials: D
authors.lastname: Ibusuki
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamashita
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D006031:Glycosylation; D006801:Humans; D008055:Lipids / Q000097:blood; D008077:Lipoproteins, LDL / Q000097:blood*; D015416:Maillard Reaction; D012016:Reference Values
chemical_list: D008055:Lipids; D008077:Lipoproteins, LDL