Surface-enhanced Raman spectroscopic studies of coactivator-associated arginine methyltransferase 1.
We report, for the first time, the surface-enhanced Raman spectra of an important enzyme, coactivator-associated arginine methyltransferase 1 (CARM1), involved in various biological activities such as tumor suppressor function and stem cell differentiation. We have employed surface-enhanced Raman scattering (SERS) to obtain insight into the structural details of CARM1 by adsorbing it to silver (Ag) nanoparticles. The enzyme retains its activity even after its adsorption onto Ag nanoparticles. We observe strong SERS modes arising from amide vibrations and aromatic ring amino acids. The SERS spectra revealed amide I bands at 1637 cm(-1) and 1666 cm(-1), which arise as a result of the alpha helix of the protein and the polypeptide backbone vibration of a random coil, respectively. In order to confirm the amide vibrations, we have performed SERS on deuterated CARM1, which exhibits a clear red shift in amide band positions. The SERS spectra may provide useful information, which could be harnessed to study the functional interactions of CARM1 with small molecule modulators.
Kumar
G V Pavan
GV
Light Scattering Laboratory, Chemistry and Physics of Material Unit, Jawaharlal Nehru Center for Advanced Scientific Research, Jakkur, Bangalore,-560064, India.
Selvi
Ruthrotha
R
Kishore
A Hari
AH
Kundu
Tapas K
TK
Narayana
Chandrabhas
C
D000327:Adsorption; D006367:HeLa Cells; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008780:Methyltransferases / Q000737:chemistry*; D053758:Nanoparticles; D011484:Protein-Arginine N-Methyltransferases; D011994:Recombinant Proteins / Q000737:chemistry; D013059:Spectrum Analysis, Raman / Q000379:methods*
D047908:Intracellular Signaling Peptides and Proteins; D011994:Recombinant Proteins; D008780:Methyltransferases; C077013:PRMT2 protein, human; D011484:Protein-Arginine N-Methyltransferases