Protein disorder is positively correlated with gene expression in Escherichia coli.
We considered, on a global scale, the relationship between the predicted fraction of protein disorder and the RNA and protein expression in Escherichia coli. Fraction of protein disorder correlated positively with both measured RNA expression levels of E. coli genes in three different growth media and with predicted abundance levels of E. coli proteins. Though weak, the correlation was highly significant. Correlation of protein disorder with RNA expression did not depend on the growth rate of E. coli cultures and was not caused by a small subset of genes showing exceptionally high concordance in their disorder and expression levels. Global analysis was complemented by detailed consideration of several groups of proteins.
Paliy
Oleg
O
Department of Biochemistry and Molecular Biology, Wright State University, Dayton, Ohio 45435, USA. oleg.paliy@wright.edu
Gargac
Shawn M
SM
Cheng
Yugong
Y
Uversky
Vladimir N
VN
Dunker
A Keith
AK
D000103:Acetyl-CoA Carboxylase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000213:Acyl Carrier Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D000217:Acyltransferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001059:Apoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D025243:Bacterial Proton-Translocating ATPases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018835:Chaperonin 10 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D003470:Culture Media / Q000494:pharmacology; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051047:Dihydrolipoyllysine-Residue Acetyltransferase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D054889:Fatty Acid Synthase, Type II; D020869:Gene Expression Profiling; D015964:Gene Expression Regulation, Bacterial; D050961:Glycine Decarboxylase Complex H-Protein / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008074:Lipoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018832:Molecular Chaperones / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010445:Peptide Elongation Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010457:Peptidoglycan / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D039861:Prokaryotic Initiation Factor-1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011487:Protein Conformation; D012269:Ribosomal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D035602:Transcriptional Elongation Factors
D000213:Acyl Carrier Protein; D001059:Apoproteins; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D002352:Carrier Proteins; D018835:Chaperonin 10; D003470:Culture Media; D004268:DNA-Binding Proteins; D029968:Escherichia coli Proteins; C031163:ExcC protein, E coli; D050961:Glycine Decarboxylase Complex H-Protein; C031908:H-NS protein, bacteria; D008074:Lipoproteins; D018832:Molecular Chaperones; C070291:NusG protein, E coli; D010445:Peptide Elongation Factors; D010457:Peptidoglycan; D039861:Prokaryotic Initiation Factor-1; D012269:Ribosomal Proteins; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; C040157:acpP protein, E coli; C068727:nusA protein, E coli; C072721:Skp protein, E coli; D000217:Acyltransferases; D051047:Dihydrolipoyllysine-Residue Acetyltransferase; C021515:dihydrolipoamide succinyltransferase; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System; C111232:RNA polymerase omega subunit; D012321:DNA-Directed RNA Polymerases; D025243:Bacterial Proton-Translocating ATPases; D054889:Fatty Acid Synthase, Type II; D000103:Acetyl-CoA Carboxylase; C079633:biotin carboxyl carrier protein