Identification of human hnRNP C1/C2 as a dengue virus NS1-interacting protein.
Dengue virus nonstructural protein 1 (NS1) is a key glycoprotein involved in the production of infectious virus and the pathogenesis of dengue diseases. Very little is known how NS1 interacts with host cellular proteins and functions in dengue virus-infected cells. This study aimed at identifying NS1-interacting host cellular proteins in dengue virus-infected cells by employing co-immunoprecipitation, two-dimensional gel electrophoresis, and mass spectrometry. Using lysates of dengue virus-infected human embryonic kidney cells (HEK 293T), immunoprecipitation with an anti-NS1 monoclonal antibody revealed eight isoforms of dengue virus NS1 and a 40-kDa protein, which was subsequently identified by quadrupole time-of-flight tandem mass spectrometry (Q-TOF MS/MS) as human heterogeneous nuclear ribonucleoprotein (hnRNP) C1/C2. Further investigation by co-immunoprecipitation and co-localization confirmed the association of hnRNP C1/C2 and dengue virus NS1 proteins in dengue virus-infected cells. Their interaction may have implications in virus replication and/or cellular responses favorable to survival of the virus in host cells.
Noisakran
Sansanee
S
Medical Biotechnology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand.
Sengsai
Suchada
S
Thongboonkerd
Visith
V
Kanlaya
Rattiyaporn
R
Sinchaikul
Supachok
S
Chen
Shui-Tein
ST
Puttikhunt
Chunya
C
Kasinrerk
Watchara
W
Limjindaporn
Thawornchai
T
Wongwiwat
Wiyada
W
Malasit
Prida
P
Yenchitsomanus
Pa-thai
PT
D000911:Antibodies, Monoclonal / Q000276:immunology; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism / Q000821:virology; D003593:Cytoplasm / Q000378:metabolism / Q000821:virology; D003716:Dengue Virus / Q000502:physiology*; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D020033:Protein Isoforms / Q000032:analysis / Q000378:metabolism; D040901:Proteomics; D053719:Tandem Mass Spectrometry; D017361:Viral Nonstructural Proteins / Q000032:analysis / Q000378:metabolism*; D014779:Virus Replication
D000911:Antibodies, Monoclonal; D034482:Heterogeneous-Nuclear Ribonucleoprotein Group C; C079340:NS1 protein, Dengue virus type 2; D020033:Protein Isoforms; D017361:Viral Nonstructural Proteins