Selective accumulation of PpIX and photodynamic effect after aminolevulinic acid treatment of human adenomyosis xenografts in nude mice.
OBJECTIVE
To evaluate the effect of photodynamic therapy with aminolevulinic acid (ALA) on human adenomyosis xenografts in a mouse model.


METHODS
Human adenomyosis tissues were implanted SC into nude mice. We measured 5-aminolevulinic acid pharmacokinetics in these mice by analyzing tissue sections 1 to 6 hours after intraperitoneal administration. Twenty-four hours after photodynamic therapy, we evaluated tissue morphologic features.


METHODS
Department of obstetrics and gynecology at a university hospital in Japan.


METHODS
Immunodeficient mice. Tissue grafts were taken from women with adenomyosis attending a university hospital.


METHODS
Photodynamic treatment.


METHODS
Peak fluorescence after intraperitoneal ALA administration and tissue histological changes 24 hours after photodynamic therapy.


RESULTS
Peak fluorescence was observed 3 hours after intraperitoneal administration. Histological studies revealed decreased numbers of epithelial and stromal cells in adenomyosis models after therapy.


CONCLUSIONS
Photodynamic therapy with ALA caused extensive cell death in human adenomyosis tissues implanted into nude mice. Photodynamic treatment using ALA is a potential treatment for patients with adenomyosis uteri.
Suzuki-Kakisaka
Haruka
H
Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Japan. suharuka@mail.tains.tohoku.ac.jp
Murakami
Takashi
T
Hirano
Toru
T
Terada
Yukihiro
Y
Yaegashi
Nobuo
N
Okamura
Kunihiro
K
D000328:Adult; D000622:Aminolevulinic Acid / Q000008:administration & dosage*; D000818:Animals; D004715:Endometriosis / Q000188:drug therapy* / Q000378:metabolism; D005260:Female; D006801:Humans; D007274:Injections, Intraperitoneal; D007564:Japan; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D010778:Photochemotherapy; D011524:Protoporphyrins / Q000378:metabolism*; D014183:Transplantation, Heterologous
D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX