Enhancement of apoptosis by sonodynamic therapy with protoporphyrin IX in isolate sarcoma 180 cells.
This study was to investigate whether the apoptosis in isolate sarcoma 180 (S180) cells could be enhanced by ultrasound in the presence of protoporphyrin IX (PPIX) and also to evaluate the underlying biologic mechanism. S180 cells were exposed to ultrasound for 30 seconds' duration, at the frequency of 2.2 MHz and an acoustical power of 3 W/cm(2) with 120 microM of PPIX. Cell apoptosis was evaluated based on the morphologic changes at different incubation times after sonication. Our results showed that the apoptosis in S180 tumor cells were induced by exposure, and the rate of apoptosis rose gradually with a longer incubation time. Our results also showed that changes in Fas protein expression were correlated with the development of apoptosis. The activities of caspase-8 and -3 were apparently upregulated by apoptosis, and the death substrate (PARP) was cleaved to active segments in a time-dependent manner. These results indicated that PPIX-mediated sonodynamic effect could exert its antitumor effect by triggering apoptosis in S180 cells through a Fas-mediated signal transduction pathway.
Wang
Xiao Bing
XB
College of Life Sciences, Shaanxi Normal University, Xi'an, China.
Liu
Quan Hong
QH
Wang
Pan
P
Zhang
Kun
K
Tang
Wei
W
Wang
Bao Lu
BL
D000818:Animals; D017304:Annexin A5 / Q000737:chemistry / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D053181:Caspase 8 / Q000378:metabolism; D045744:Cell Line, Tumor; D002469:Cell Separation; D024161:Collagen Type XI / Q000378:metabolism; D004789:Enzyme Activation; D016650:Fluorescein-5-isothiocyanate; D051379:Mice; D008813:Mice, Inbred ICR; D011524:Protoporphyrins / Q000494:pharmacology*; D012510:Sarcoma 180 / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014465:Ultrasonics
D017304:Annexin A5; C499319:COL11A2 protein, human; D024161:Collagen Type XI; D011524:Protoporphyrins; C028025:protoporphyrin IX; D053148:Caspase 3; D053181:Caspase 8; D016650:Fluorescein-5-isothiocyanate