Rice-arsenate interactions in hydroponics: a three-gene model for tolerance.
In this study, the genetic mapping of the tolerance of root growth to 13.3 muM arsenate [As(V)] using the BalaxAzucena population is improved, and candidate genes for further study are identified. A remarkable three-gene model of tolerance is advanced, which appears to involve epistatic interaction between three major genes, two on chromosome 6 and one on chromosome 10. Any combination of two of these genes inherited from the tolerant parent leads to the plant having tolerance. Lists of potential positional candidate genes are presented. These are then refined using whole genome transcriptomics data and bioinformatics. Physiological evidence is also provided that genes related to phosphate transport are unlikely to be behind the genetic loci conferring tolerance. These results offer testable hypotheses for genes related to As(V) tolerance that might offer strategies for mitigating arsenic (As) accumulation in consumed rice.
Norton
Gareth J
GJ
Department of Plant and Soil Science, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen AB24 3UU, UK. g.norton@abdn.ac.uk
Nigar
Meher
M
Williams
Paul N
PN
Dasgupta
Tapash
T
Meharg
Andrew A
AA
Price
Adam H
AH
D001149:Arsenates / Q000378:metabolism* / Q000494:pharmacology; D032461:Chromosomes, Plant / Q000235:genetics; D017343:Genes, Plant / Q000187:drug effects*; D018527:Hydroponics; D007700:Kinetics; D012275:Oryza / Q000187:drug effects* / Q000235:genetics* / Q000502:physiology; D010710:Phosphates / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D017385:Sequence Homology; D014158:Transcription, Genetic / Q000187:drug effects
D001149:Arsenates; D010710:Phosphates; C025657:arsenic acid