Mode of action of human beta-defensin 3 against Staphylococcus aureus and transcriptional analysis of responses to defensin challenge.
Human beta-defensin 3 (hBD3) is a highly charged (+11) cationic defence peptide, which retains activity against staphylococci even at elevated salt concentrations. We studied the antibiotic mode of action of hBD3 against Staphylococcus aureus SG511, using whole-cell assays and analysing the transcriptional response to hBD3 treatment. hBD3 caused rapid killing and simultaneously blocked all biosynthetic pathways, however, significant depolarisation was not observed and permeabilisation of the membrane was incomplete. The transcriptional response pattern was in part similar to those of strongly cationic amphiphiles, e.g. in that anaerobic energy production was downregulated. Significantly, part of the staphylococcal cell wall stress stimulon were upregulated. The most prominent microbial counterstrategy appears to be based on the upregulation of ABC transporters possibly functioning in detoxification of the membrane environment; the transporter genes were highly upregulated in both, a short-term response and a long-term adaptation experiment. Knockout of the most highly induced transporter VraDE significantly enhanced hBD3 susceptibility. We propose that the antibiotic activity of hBD3 is based on interference with the organisation over space and time of membrane-bound multienzyme machineries such as the electron transport chain and, in particular, the cell wall biosynthesis complex rather than on formation of defined transmembrane pores.
Sass
Vera
V
Institute of Medical Microbiology, Immunology and Parasitology - Pharmaceutical Microbiology Section, University of Bonn, Bonn, Germany.
Pag
Ulrike
U
Tossi
Alessandro
A
Bierbaum
Gabriele
G
Sahl
Hans-Georg
HG
D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001693:Biological Transport, Active / Q000187:drug effects; D002473:Cell Wall / Q000378:metabolism; D015169:Colony Count, Microbial; D004734:Energy Metabolism / Q000187:drug effects; D017353:Gene Deletion; D020869:Gene Expression Profiling; D005798:Genes, Bacterial; D006801:Humans; D026901:Membrane Transport Proteins / Q000096:biosynthesis; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability; D020411:Oligonucleotide Array Sequence Analysis; D014176:Protein Biosynthesis / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics; D023083:beta-Defensins / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D026901:Membrane Transport Proteins; D023083:beta-Defensins