title: Inhibition of catalase by aminotriazole in vivo results in reduction of glucose-6-phosphate dehydrogenase activity in Saccharomyces cerevisiae cells.
abstract: The inhibitor of catalase 3-amino-1,2,4-triazole (AMT) was used to study the physiological role of catalase in the yeast Saccharomyces cerevisiae under starvation. It was shown that AMT at the concentration of 10 mM did not affect the growth of the yeast. In vivo and in vitro the degree of catalase inhibition by AMT was concentration- and time-dependent. Peroxisomal catalase in bakers' yeast was more sensitive to AMT than the cytosolic one. In vivo inhibition of catalase by AMT in S. cerevisiae caused a simultaneous decrease in glucose-6-phosphate dehydrogenase activity and an increase in glutathione reductase activity. At the same time, the level of protein carbonyls, a marker of oxidative modification, was not affected. Possible mechanisms compensating the negative effects caused by AMT inhibition of catalase are discussed.
authors.affiliation: Department of Biochemistry, Vassyl Stefanyk Precarpathian National University, Ivano-Frankivsk 76025, Ukraine.
authors.forename: M
authors.initials: M
authors.lastname: Bayliak
authors.forename: D
authors.initials: D
authors.lastname: Gospodaryov
authors.forename: H
authors.initials: H
authors.lastname: Semchyshyn
authors.forename: V
authors.initials: V
authors.lastname: Lushchak
mesh_terms: D000640:Amitrole / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000037:antagonists & inhibitors* / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000201:enzymology*
chemical_list: D004791:Enzyme Inhibitors; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D000640:Amitrole