title: Selective accumulation of PpIX and photodynamic effect after aminolevulinic acid treatment of human adenomyosis xenografts in nude mice.
abstract: OBJECTIVE
  To evaluate the effect of photodynamic therapy with aminolevulinic acid (ALA) on human adenomyosis xenografts in a mouse model.
  
  
  METHODS
  Human adenomyosis tissues were implanted SC into nude mice. We measured 5-aminolevulinic acid pharmacokinetics in these mice by analyzing tissue sections 1 to 6 hours after intraperitoneal administration. Twenty-four hours after photodynamic therapy, we evaluated tissue morphologic features.
  
  
  METHODS
  Department of obstetrics and gynecology at a university hospital in Japan.
  
  
  METHODS
  Immunodeficient mice. Tissue grafts were taken from women with adenomyosis attending a university hospital.
  
  
  METHODS
  Photodynamic treatment.
  
  
  METHODS
  Peak fluorescence after intraperitoneal ALA administration and tissue histological changes 24 hours after photodynamic therapy.
  
  
  RESULTS
  Peak fluorescence was observed 3 hours after intraperitoneal administration. Histological studies revealed decreased numbers of epithelial and stromal cells in adenomyosis models after therapy.
  
  
  CONCLUSIONS
  Photodynamic therapy with ALA caused extensive cell death in human adenomyosis tissues implanted into nude mice. Photodynamic treatment using ALA is a potential treatment for patients with adenomyosis uteri.
authors.affiliation: Department of Obstetrics and Gynecology, Tohoku University Graduate School of Medicine, Sendai, Japan. suharuka@mail.tains.tohoku.ac.jp
authors.forename: Haruka
authors.initials: H
authors.lastname: Suzuki-Kakisaka
authors.forename: Takashi
authors.initials: T
authors.lastname: Murakami
authors.forename: Toru
authors.initials: T
authors.lastname: Hirano
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Terada
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yaegashi
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Okamura
mesh_terms: D000328:Adult; D000622:Aminolevulinic Acid / Q000008:administration & dosage*; D000818:Animals; D004715:Endometriosis / Q000188:drug therapy* / Q000378:metabolism; D005260:Female; D006801:Humans; D007274:Injections, Intraperitoneal; D007564:Japan; D051379:Mice; D008819:Mice, Nude; D008875:Middle Aged; D010778:Photochemotherapy; D011524:Protoporphyrins / Q000378:metabolism*; D014183:Transplantation, Heterologous
chemical_list: D011524:Protoporphyrins; D000622:Aminolevulinic Acid; C028025:protoporphyrin IX