A live attenuated severe acute respiratory syndrome coronavirus is immunogenic and efficacious in golden Syrian hamsters.
The immunogenicity and protective efficacy of a live attenuated vaccine consisting of a recombinant severe acute respiratory syndrome (SARS) coronavirus lacking the E gene (rSARS-CoV-DeltaE) were studied using hamsters. Hamsters immunized with rSARS-CoV-DeltaE developed high serum-neutralizing antibody titers and were protected from replication of homologous (SARS-CoV Urbani) and heterologous (GD03) SARS-CoV in the upper and lower respiratory tract. rSARS-CoV-DeltaE-immunized hamsters remained active following wild-type virus challenge, while mock-immunized hamsters displayed decreased activity. Despite being attenuated in replication in the respiratory tract, rSARS-CoV-DeltaE is an immunogenic and efficacious vaccine in hamsters.
Lamirande
Elaine W
EW
Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA.
DeDiego
Marta L
ML
Roberts
Anjeanette
A
Jackson
Jadon P
JP
Alvarez
Enrique
E
Sheahan
Tim
T
Shieh
Wun-Ju
WJ
Zaki
Sherif R
SR
Baric
Ralph
R
Enjuanes
Luis
L
Subbarao
Kanta
K
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D006224:Cricetinae; D017353:Gene Deletion; D008168:Lung / Q000473:pathology / Q000821:virology; D008647:Mesocricetus; D009500:Neutralization Tests; D012137:Respiratory System / Q000821:virology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control*; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D000086082:Viroporin Proteins
D000914:Antibodies, Viral; C501689:E protein, SARS coronavirus; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; D000086082:Viroporin Proteins