Changes in the de novo, salvage, and degradation pathways of pyrimidine nucleotides during tobacco shoot organogenesis.
Pyrimidine nucleotide metabolism was studied in tobacco callus cultured for 21days under shoot-forming (SF) and non-shoot-forming (NSF) conditions by following the metabolic fate of orotic acid, a precursor of the de novo pathway, and uridine and uracil, intermediates of the salvage and degradation pathways respectively. Nucleic acid synthesis was also investigated by measuring the incorporation of labeled thymidine into different cellular components. Our results indicate that with respect to nucleotide metabolism, the organogenic process in tobacco can be divided in two "metabolic phases": a de novo phase followed by a salvage phase. The initial stages of meristemoid formation during tobacco organogenesis (up to day 8) are characterized by a heavy utilization of orotic acid into nucleotides and nucleic acids. Utilization of this intermediate for the de novo synthesis of nucleotides, which is limited in NSF tissue, is mainly due to the activity of orotate phosphoribosyltransferase (OPRT), which increases in tissue cultured under SF conditions. After day 8, nucleotide synthesis during shoot growth seems to be mainly due to the salvage activity of both uridine and uracil. Both intermediates are preferentially utilized in SF tissue for the formation of nucleotides and nucleic acids through the activities of their respective salvage enzymes: uridine kinase (URK), and uracil phosphoribosyltransferase (UPRT). Metabolic studies on thymidine indicate that in SF tissue maximal nucleic acid synthesis occurs at day 4, in support of the initiation of meristemoid formation. Overall these results suggest that the organogenic process in tobacco is underlined by precise fluctuations in pyrimidine metabolism which delineate structural events culminating in shoot formation.
Loukanina
Natalia
N
Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Stasolla
Claudio
C
Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, 3RT 2N2, Canada.
Belmonte
Mark F
MF
Department of Plant Science, University of Manitoba, Winnipeg, Manitoba, 3RT 2N2, Canada.
Yeung
Edward C
EC
Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
Thorpe
Trevor A
TA
Plant Physiology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada.
D018506:Gene Expression Regulation, Plant; D008954:Models, Biological; D009962:Orotate Phosphoribosyltransferase / Q000235:genetics / Q000378:metabolism; D010430:Pentosyltransferases / Q000235:genetics / Q000378:metabolism; D018520:Plant Shoots / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D011742:Pyrimidine Nucleotides / Q000378:metabolism*; D011743:Pyrimidines / Q000378:metabolism*; D013936:Thymidine / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D014498:Uracil / Q000378:metabolism; D014529:Uridine / Q000378:metabolism; D014541:Uridine Kinase / Q000235:genetics / Q000378:metabolism
D011742:Pyrimidine Nucleotides; D011743:Pyrimidines; D014498:Uracil; D010430:Pentosyltransferases; D009962:Orotate Phosphoribosyltransferase; C023269:uracil phosphoribosyltransferase; D014541:Uridine Kinase; D013936:Thymidine; D014529:Uridine