title: 3,4-DGE in peritoneal dialysis fluids cannot be found in plasma after infusion into the peritoneal cavity.
abstract: OBJECTIVE
  Glucose degradation products (GDPs) are important in the outcome of peritoneal dialysis (PD) treatment. 3,4-dideoxyglucosone-3-ene (3,4-DGE) is the most cytotoxic GDP found in conventionally manufactured fluids and may, in addition, be recruited from 3-deoxyglucosone (3-DG). It is not known what happens with those GDPs in patients during PD. The aim of this study was to investigate if the 3,4-DGE and 3-DG in PD fluids can be found in plasma during treatment.
  
  
  METHODS
  PD patients were dialyzed with a conventional PD fluid containing 43 micromol/L 3,4-DGE and 281 micromol/L 3-DG. Parallel experiments were performed in rats as well as in vitro with human plasma. The rats were dialyzed with a PD fluid containing 100 micromol/L 3,4-DGE and 200 micromol/L 3-DG.
  
  
  RESULTS
  The concentration of 3,4-DGE in the peritoneum decreased at a much higher rate than 3-DG during the dwell. 3,4-DGE was not, however, detected in the plasma of patients or rats during dialysis. The concentration of 3-DG in plasma peaked shortly after infusion of the fluid to the peritoneal cavity. The concentration of 3,4-DGE during experimental incubation in plasma decreased rapidly, while the concentration of 3-DG decreased only 10% as rapidly or less.
  
  
  CONCLUSIONS
  3,4-DGE could not be detected in plasma from either PD patients or rats during dialysis. This is presumably due to its high reactivity. 3-DG may, on the other hand, pass through the membrane and be detected in the blood.
authors.affiliation: Gambro AB, Lund, Sweden. martin.erixon@gambro.com
authors.forename: Martin
authors.initials: M
authors.lastname: Erixon
authors.forename: Anders
authors.initials: A
authors.lastname: Wieslander
authors.forename: Torbjörn
authors.initials: T
authors.lastname: Lindén
authors.forename: Ola
authors.initials: O
authors.lastname: Carlsson
authors.forename: Jan Ake
authors.initials: JA
authors.lastname: Jönsson
authors.forename: Ole
authors.initials: O
authors.lastname: Simonsen
authors.forename: Per
authors.initials: P
authors.lastname: Kjellstrand
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003847:Deoxyglucose / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000097:blood; D015314:Dialysis Solutions / Q000737:chemistry* / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D007676:Kidney Failure, Chronic / Q000378:metabolism / Q000628:therapy*; D010530:Peritoneal Dialysis; D011753:Pyrones / Q000008:administration & dosage / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C504711:3,4-dideoxyglucosone-3-ene; D015314:Dialysis Solutions; D011753:Pyrones; D003847:Deoxyglucose; C016350:3-deoxyglucosone