title: In vitro differentiation of canine celiac adipose tissue-derived stromal cells into neuronal cells.
abstract: To investigate in vitro differentiation of canine adipose tissue-derived stromal cells (ATSCs) into neuronal cells, ATSCs from celiac adipose tissue in clinically healthy beagle dogs were treated with 100 muM dibutyryl cyclic adenosine monophosphate (dbcAMP) and 125 muM isobuthylmethylxanthine (IBMX). ATSCs were morphologically changed into differentiated ATSCs from spindle-shaped cells to neuron-like cells with numerous processes after the treatment. Expression of neuron-specific enolase (NSE) as an early neuron specific marker protein was detected in both ATSCs and differentiated ATSCs, however diachronic increase of NSE expression was observed in differentiated ATSCs after the treatment with dbcAMP/IBMX. In addition, neurofilament-68 (NF-68) as an early to mature neuron specific marker protein was weakly expressed in differentiated ATSCs. Neuron specific glutamate and glucose transporter (EAAC1 and GLUT-3, respectively) mRNAs were strongly expressed in differentiated ATSCs compared with those in ATSCs, although glia specific glutamate transporter mRNA (GLT-1) was also detected in differentiated ATSCs. ATSCs can differentiate into early to mature neuronal cells and are candidate cells for autologous nerve regeneration therapy, although additional research is needed to examine functional characteristics of differentiated ATSCs.
authors.affiliation: Department of Veterinary Clinical Pathobiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Sago
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tamahara
authors.forename: Mizuki
authors.initials: M
authors.lastname: Tomihari
authors.forename: Naoaki
authors.initials: N
authors.lastname: Matsuki
authors.forename: Yukiho
authors.initials: Y
authors.lastname: Asahara
authors.forename: Akihiro
authors.initials: A
authors.lastname: Takei
authors.forename: Makoto
authors.initials: M
authors.lastname: Bonkobara
authors.forename: Tsukimi
authors.initials: T
authors.lastname: Washizu
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Ono
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000502:physiology*; D004285:Dogs; D005786:Gene Expression Regulation; D009474:Neurons / Q000166:cytology*; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D015415:Biomarkers; D010751:Phosphopyruvate Hydratase