Effects of temperature regime through premetamorphic ontogeny on shape of the chondrocranium in the American toad, Anaxyrus americanus.
If one considers the substantial amount of information that exists about phenotypic plasticity in amphibians, it is surprising that few studies have examined abiotic factors that influence phenotype through ontogeny. Phenotypic change and stability of morphology are artifacts of organisms that bear significant relevance to evolution within and among taxonomic groups. Here, we examine development as a phenotypically plastic aspect of larval anurans. Fertilized eggs of the American Toad, Anaxyrus (= Bufo) americanus (Holbrook, 1836), were obtained from two pairs of adults, and larvae were reared in four temperature treatments (constant Mean, constant High, constant Low, and Fluctuating regime [Low night-High day]); developmental series were collected from each treatment, representing larvae of this species from Gosner Stages 28-40. Cleared and stained larvae were analyzed with landmark-based geometric morphometric methods to facilitate examination of differences in overall shape change of the larval chondrocranium through ontogeny, as a result of developmental temperature or temperature regime. Changes in shape of the chondrocranium and in amount and direction of phenotypic change through ontogeny were found in response to temperature treatment and temperature regime. Mean chondrocranial shape of the Fluctuating regime was more similar to the consensus shape of the overall data set than were those of all other treatments. Given that differences in amount and direction of shape change were observed among these treatments and throughout ontogeny, one should consider the affects of abiotic factors (such as temperature) when rearing larval anurans for studies of developmental morphology.
Jorgensen
Michael E
ME
Department of Biology, John Carroll University, University Heights, Ohio 45701, USA. mj207406@ohio.edu
Sheil
Christopher A
CA
D000818:Animals; D002025:Bufonidae / Q000033:anatomy & histology* / Q000254:growth & development*; D002356:Cartilage / Q000033:anatomy & histology* / Q000254:growth & development*; D005260:Female; D007814:Larva / Q000033:anatomy & histology / Q000254:growth & development; D008297:Male; D008675:Metamorphosis, Biological; D015999:Multivariate Analysis; D010641:Phenotype; D012886:Skull / Q000033:anatomy & histology* / Q000254:growth & development*; D013696:Temperature