title: Adenovirus 5 and 35 vectors expressing Plasmodium falciparum circumsporozoite surface protein elicit potent antigen-specific cellular IFN-gamma and antibody responses in mice.
abstract: Falciparum malaria vaccine candidates have been developed using recombinant, replication-deficient serotype 5 and 35 adenoviruses (Ad5, Ad35) encoding the Plasmodium falciparum circumsporozoite surface protein (CSP) (Ad5.CS, Ad35.CS) (Crucell Holland BV, Leiden, The Netherlands). To evaluate the immunogenicity of these constructs, BALB/cJ mice were immunized twice with either Ad5.CS, Ad35.CS, empty Ad5-vector (eAd5), empty Ad35 vector (eAd35), or saline. Another group received the CSP-based RTS,S malaria vaccine formulated in the proprietary Adjuvant System AS01B (GlaxoSmithKline Biologicals, Rixensart, Belgium). Here we report that Ad5.CS, Ad35.CS, and RTS,S/AS01B, elicited both cellular and serologic CSP antigen-specific responses in mice. These adenoviral vectors induce strong malaria-specific immunity and warrant further evaluation.
authors.affiliation: Division of Malaria Vaccine Development, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA. Joseph.Shott@rhsp.org
authors.forename: Joseph P
authors.initials: JP
authors.lastname: Shott
authors.forename: Shannon M
authors.initials: SM
authors.lastname: McGrath
authors.forename: Maria Grazia
authors.initials: MG
authors.lastname: Pau
authors.forename: Jerome H V
authors.initials: JH
authors.lastname: Custers
authors.forename: Olga
authors.initials: O
authors.lastname: Ophorst
authors.forename: Marie-Ange
authors.initials: MA
authors.lastname: Demoitié
authors.forename: Marie-Claude
authors.initials: MC
authors.lastname: Dubois
authors.forename: Jack
authors.initials: J
authors.lastname: Komisar
authors.forename: Michelle
authors.initials: M
authors.lastname: Cobb
authors.forename: Kent E
authors.initials: KE
authors.lastname: Kester
authors.forename: Patrice
authors.initials: P
authors.lastname: Dubois
authors.forename: Joe
authors.initials: J
authors.lastname: Cohen
authors.forename: Jaap
authors.initials: J
authors.lastname: Goudsmit
authors.forename: D Gray
authors.initials: DG
authors.lastname: Heppner
authors.forename: V Ann
authors.initials: VA
authors.lastname: Stewart
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D000913:Antibodies, Protozoan / Q000032:analysis / Q000096:biosynthesis*; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors; D007115:Immunization Schedule; D007117:Immunization, Secondary; D007371:Interferon-gamma / Q000096:biosynthesis*; D017780:Malaria Vaccines / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000235:genetics* / Q000378:metabolism*; D034101:Sporozoites / Q000276:immunology*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D017780:Malaria Vaccines; D007371:Interferon-gamma