[Isolation of zygotes and apical and basal cells of bicelluare proembryo from Torenia fournieri].
Torenia fournieri is a special plant with embryo sac partly protruding through the micropyle of ovule, and the cells of egg apparatus can be clearly observed using light microscope. Zygotes and the cells of bicellular proembryo could be isolated using enzymatic digestion. In the solution containing 0.05% cellulase and 0.05% pectinase, 14-15 zygotes were isolated from 50 ovules in 1h. In the solution containing 0.2% cellulose, 0.4% hemicellulase and 0.2% pectinase, 19 pairs of apical and basal cells of bicellular proembryo could be isolated from 50 ovules in 1 h. The isolated zygotes and epical and basal cells were collected using a micromanipulator up to a number which will prepare for suing molecular methods to probe the mechanism of early embryogenesis of higher plants.
Yang
Yan Hong
YH
School of Life Sciences, Xiamen University, Xiamen 361005.
Wang
Ya Ying
YY
Ye
Zu Yun
ZY
Tian
Hui Qiao
HQ
D002469:Cell Separation / Q000379:methods*; D019684:Magnoliopsida / Q000166:cytology* / Q000196:embryology; D015053:Zygote / Q000166:cytology*