Increased osteopontin levels in the cerebrospinal fluid of patients with multiple sclerosis.
OBJECTIVE
To determine cerebrospinal fluid levels of osteopontin (OPN), a proinflammatory cytokine that was found to be overexpressed in multiple sclerosis lesions and increased in plasma during relapses and in secondary progressive multiple sclerosis.


METHODS
Case series. Osteopontin, interleukin 12p40 (IL-12p40), IL-10, and matrix metalloproteinase 9 were measured by enzyme-linked immunosorbent assay by an investigator unaware of the patients' diagnoses.


METHODS
Consecutive patients with multiple sclerosis (n = 27), or other inflammatory (n = 11) or non-inflammatory (n = 23) neurological diseases, undergoing lumbar puncture, were investigated.


RESULTS
Osteopontin was significantly elevated in the cerebrospinal fluid of patients with multiple sclerosis (mean [SD], 415 [186] ng/mL) and other inflammatory diseases (563 [411] ng/mL) compared with those with noninflammatory neurological diseases (286 [150] ng/mL). Cerebrospinal fluid OPN levels were slightly higher than plasma OPN levels. Cerebrospinal fluid OPN levels positively correlated with the ability to detect cerebrospinal fluid IL-12p40.


CONCLUSIONS
Osteopontin in the cerebrospinal fluid may be, in part, of central nervous system origin, and may play an important role in central nervous system inflammation.
Braitch
Manjit
M
Division of Clinical Neurology, University of Nottingham, Queen's Medical Centre, Nottingham, England.
Nunan
Robert
R
Niepel
Graham
G
Edwards
Laura J
LJ
Constantinescu
Cris S
CS
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid; D002490:Central Nervous System / Q000276:immunology / Q000378:metabolism / Q000503:physiopathology; D018450:Disease Progression; D004660:Encephalitis / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D018836:Inflammation Mediators / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid*; D016753:Interleukin-10 / Q000032:analysis / Q000134:cerebrospinal fluid; D053762:Interleukin-12 Subunit p40 / Q000032:analysis / Q000134:cerebrospinal fluid; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000032:analysis / Q000134:cerebrospinal fluid; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000134:cerebrospinal fluid* / Q000175:diagnosis / Q000276:immunology; D053495:Osteopontin / Q000032:analysis / Q000097:blood / Q000134:cerebrospinal fluid*; D015854:Up-Regulation / Q000276:immunology
D015415:Biomarkers; D018836:Inflammation Mediators; D053762:Interleukin-12 Subunit p40; D053495:Osteopontin; D016753:Interleukin-10; D020780:Matrix Metalloproteinase 9