title: Psychotropic drug tenoten activates mitogen-activated MAP/ERK kinase regulatory cascade controlling the neuroprotective effects.
abstract: For evaluation of the molecular mechanisms underlying the effects of tenoten, a preparation based on ultralow-dose antibodies to S100 proteins and intended for the therapy of anxious and depressive disorders, we studied its influence on mitogen-activated ERK kinase cascade in Helix lucorum subpharyngeal ganglion complex. Western blot analysis showed that incubation of the ganglion preparation with tenoten resulted in significant activation of mitogen-activated ERK kinases, which was reduced by PD98059 (blocker of upstream protein kinase). This attests to a specific effect of the drug on mitogen-activated ERK kinase cascade. It is hypothesized that the therapeutic efficiency of tenoten is realed to activation of mitogen-activated ERK kinase cascade.
authors.affiliation: Materia Medica Holding, Moscow.
authors.forename: O I
authors.initials: OI
authors.lastname: Epstein
authors.forename: O V
authors.initials: OV
authors.lastname: Vorobieva
authors.forename: L N
authors.initials: LN
authors.lastname: Grinkevich
authors.forename: O A
authors.initials: OA
authors.lastname: Kharchenko
authors.forename: M B
authors.initials: MB
authors.lastname: Shtark
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism* / Q000627:therapeutic use; D001007:Anxiety / Q000188:drug therapy / Q000378:metabolism; D003866:Depressive Disorder / Q000188:drug therapy / Q000378:metabolism; D004789:Enzyme Activation; D004791:Enzyme Inhibitors / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism; D005724:Ganglia / Q000378:metabolism; D006372:Helix, Snails; D006801:Humans; D020935:MAP Kinase Signaling System / Q000502:physiology*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000378:metabolism*; D011619:Psychotropic Drugs / Q000378:metabolism* / Q000627:therapeutic use; D009418:S100 Proteins / Q000276:immunology
chemical_list: D000906:Antibodies; D004791:Enzyme Inhibitors; D005419:Flavonoids; D018696:Neuroprotective Agents; D011619:Psychotropic Drugs; D009418:S100 Proteins; C529609:tenoten; D048049:Extracellular Signal-Regulated MAP Kinases; D020928:Mitogen-Activated Protein Kinases; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one