Mechanism of growth inhibitory effect of Blumea balsamifera extract in hepatocellular carcinoma.
Blumea balsamifera is known to improve physiological disorders such as rheumatism and hypertension, but its anticancer activity has not been well elucidated. In this study, we found that Blumea balsamifera MeOH extract (BME) induced growth-inhibitory activity in rat and human hepatocellular carcinoma cells without cytotoxicity in rat hepatocytes which were used as a normal cell model. BME induced cell cycle arrest at the G1 phase via decreases in the expression of cyclin-E and phosphorylation of retinoblastoma protein. Furthermore, BME reduced the level of a proliferation-inducing ligand, that stimulates tumor cell growth. These findings suggest that BME has possible therapeutic potential in hepatoma cancer patients and that depletion of cellular APRIL is an important mechanism in the growth-inhibitory effect of BME.
Norikura
Toshio
T
Department of Food and Human Health Sciences, Graduate School of Human Life Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka 558-8585, Japan.
Kojima-Yuasa
Akiko
A
Shimizu
Miki
M
Huang
Xuedan
X
Xu
Shenghui
S
Kametani
Saeda
S
Rho
Sook-Nyung
SN
Kennedy
David Opare
DO
Matsui-Yuasa
Isao
I
D000818:Animals; D019659:Asteraceae / Q000737:chemistry*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000235:genetics / Q000473:pathology*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D019927:Cyclin E / Q000378:metabolism; D000431:Ethanol / Q000737:chemistry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006131:Growth Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D000432:Methanol / Q000737:chemistry; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology / Q000627:therapeutic use; D010766:Phosphorylation / Q000187:drug effects; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D051381:Rats; D012175:Retinoblastoma / Q000378:metabolism; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13 / Q000378:metabolism
D019927:Cyclin E; D006131:Growth Inhibitors; D019384:Nucleic Acid Synthesis Inhibitors; D010936:Plant Extracts; D053300:Tumor Necrosis Factor Ligand Superfamily Member 13; D000431:Ethanol; D000432:Methanol