Long-term maintenance of donor-derived hematopoiesis by intra-bone marrow-bone marrow transplantation.
The long-term maintenance of hematopoietic stem cells (HSCs) is assessed by serial bone marrow transplantation (BMT), in which HSCs are injected intravenously. Recently, we have found that intra-bone marrow (IBM)-BMT can efficiently reconstitute the hematopoietic system with cells of donor origin, in contrast to conventional intravenous (i.v.) BMT. In the present study, we have compared the long-term maintenance of HSCs using multiple rounds of serial i.v.-BMT and IBM-BMT. The frequencies of donor-derived progenitor cells (Lin(-)/c-kit(+) cells) and more primitive progenitors (Lin(-)/c-kit(+)/CD34(+)/Sca-1(+) cells) were higher in the tertiary recipients by serial IBM-BMT than in those that had received bone marrow cells by serial i.v.-BMT. Furthermore, neither donor-derived progenitor cells nor mature hematolymphoid cells were detected in approximately 25% of the tertiary recipients after serial i.v.-BMT, indicating that progenitor cells can be efficiently maintained by IBM-BMT but not by i.v.-BMT. Finally, we confirmed that the recipients treated with the primary IBM-BMT (without carrying out serial BMT) showed a significantly higher survival rate than those treated with i.v.-BMT. These findings clearly show that IBM-BMT efficiently promotes the longterm maintenance of donor-derived hematopoiesis.
Omae
Mariko
M
First Department of Pathology, Kansai Medical University, Osaka, Japan.
Inaba
Muneo
M
Sakaguchi
Yutaku
Y
Tsuda
Masanobu
M
Miyake
Takashi
T
Fukui
Jyunichi
J
Iwai
Hiroshi
H
Yamashita
Toshio
T
Ikehara
Susumu
S
D000818:Animals; D001853:Bone Marrow / Q000502:physiology*; D016026:Bone Marrow Transplantation / Q000379:methods* / Q000401:mortality / Q000502:physiology; D049109:Cell Proliferation; D005260:Female; D006085:Graft Survival; D006410:Hematopoiesis / Q000502:physiology*; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008954:Models, Biological; D016019:Survival Analysis; D013997:Time Factors; D014019:Tissue Donors; D018183:Transplantation Chimera / Q000502:physiology