Color development time of the Lowry protein assay.
Color development of the Lowry protein assay was tracked over time for bovine serum albumin (BSA) concentrations ranging from 40 to 600 microg/ml. The time interval between 2 and 4h produced the most stable readings. This time frame also improved linearity of the standard curve.
Pomory
Christopher M
CM
Department of Biology, University of West Florida, Pensacola, FL 32514, USA. cpomory@uwf.edu
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002417:Cattle; D003116:Color; D012015:Reference Standards; D012710:Serum Albumin, Bovine / Q000032:analysis*; D012996:Solutions; D013053:Spectrophotometry; D013997:Time Factors
D012996:Solutions; D012710:Serum Albumin, Bovine