title: Quadruplex PCR for simultaneous detection of serotype, biotype, toxigenic potential, and central regulating factor of Vibrio cholerae.
abstract: A quadruplex PCR was developed for the simultaneous detection of genes specific for Vibrio cholerae O1 and/or O139 serogroup (wbe and/or wbf), cholera toxin A subunit (ctxA), toxin-coregulated pilus (tcpA), and central regulating protein ToxR (toxR) in a single tube reaction. This is a simple, rapid, and accurate approach for the detection of toxigenic V. cholerae O1 and/or O139 and can prevent the rapid spread of the disease by early detection.
authors.affiliation: Regional Medical Research Centre, Chandrasekharpur, Bhubaneswar, Orissa, India.
authors.forename: Hemant Kumar
authors.initials: HK
authors.lastname: Khuntia
authors.forename: Bibhuti Bhusan
authors.initials: BB
authors.lastname: Pal
authors.forename: Guru Prasada
authors.initials: GP
authors.lastname: Chhotray
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics; D002771:Cholera / Q000382:microbiology; D002772:Cholera Toxin / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D004587:Electrophoresis, Agar Gel; D036781:Fimbriae Proteins / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014157:Transcription Factors / Q000235:genetics; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics*; D041242:Vibrio cholerae O139 / Q000145:classification* / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C085807:TcpA protein, Vibrio cholerae; D014157:Transcription Factors; C499324:toxR protein, Vibrio cholerae; D036781:Fimbriae Proteins; D002772:Cholera Toxin