title: The modified Je-Ho-Tang, Korean herbal medicine, inhibits whole-blood aggregation and platelet adhesion to collagen under flow.
abstract: We investigated the in vitro effect of modified Je-Ho-Tang (MJHT) hot-water extract on platelet aggregation and activation induced by agonists in human whole blood, and on platelet adhesion to a collagen-coated surface under flow shear stress conditions. MJHT extract potently inhibited the collagen-induced platelet aggregation in human whole blood in a dose-dependent manner, with a half-maximal inhibitory dose (IC(50)) value of 526 microg/mL. We accessed several markers of platelet activation using receptor expression on platelet membranes, including GPIIb/IIIa-like expression (PAC-1) and P-selectin (CD62); we monitored the intracellular calcium mobilization response by flow cytometry in healthy subjects. A significant decrease in PAC-1 (P=0.002), CD62 (P=0.002), and intracellular calcium mobilization (P<0.001) were seen in the presence of MJHT extract. In addition, we have used image analysis to study human platelet adhesion to collagen under physiologic flow conditions in a perfusion chamber. MJHT extract markedly decreased platelet adhesion to the collagen-coated surface. These results show that MJHT has potent anti-platelet activity.
authors.affiliation: Department of Herbal Resources Research, Korea Institute of Oriental Medicine, South Korea.
authors.forename: Won Kyung
authors.initials: WK
authors.lastname: Jeon
authors.forename: Yeong Eun
authors.initials: YE
authors.lastname: Kim
authors.forename: Sun Ok
authors.initials: SO
authors.lastname: Park
authors.forename: Dae Young
authors.initials: DY
authors.lastname: Kwon
authors.forename: Sang Woo
authors.initials: SW
authors.lastname: Ahn
authors.forename: Jeong Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Myoung Soon
authors.initials: MS
authors.lastname: Ji
authors.forename: Byoung Seob
authors.initials: BS
authors.lastname: Ko
mesh_terms: D000328:Adult; D002118:Calcium / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology*; D005260:Female; D006801:Humans; D055670:Medicine, Korean Traditional; D010936:Plant Extracts / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D003094:Collagen; D002118:Calcium