Evaluation of mosquito densoviruses for controlling Aedes aegypti (Diptera: Culicidae): variation in efficiency due to virus strain and geographic origin of mosquitoes.
Four mosquito densovirus strains were assayed for mortality and infectivity against Aedes aegypti larvae from different geographic regions. The viral titers were quantified by real-time PCR using TaqMan technology. Firstinstar larvae were exposed to the same titer of each densovirus strain for 48 hours. All strains of densoviruses exhibited larvicidal activity and caused more than 80% mortality and infectivity in the three mosquito strains. AalDNV-exposed larvae had the highest mortality rate. The mean time to death of AalDNV-exposed larvae was shorter than other DNVs-exposed larvae. We can conclude that different densovirus strains exhibit some variations in their pathogenicity to different populations of Ae. aegypti mosquitoes. A few mosquitoes from Chachoengsao and Bangkok exposed to AeDNV and AThDNV survived to the adult stage to lay eggs and showed 22% to 50% vertical transmission in the F1 generation. Phylogenetic analysis of four densovirus strains indicated that mosquito densoviruses are separated into two distinct clades.
Hirunkanokpun
Supanee
S
Center of Excellence for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University at Salaya, Nakhonpathom, Thailand.
Carlson
Jonathan O
JO
Kittayapong
Pattamaporn
P
D000330:Aedes / Q000235:genetics / Q000254:growth & development / Q000821:virology*; D000818:Animals; D004247:DNA / Q000302:isolation & purification; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D019223:Densovirinae / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D005843:Geography; D007814:Larva / Q000821:virology; D010572:Pest Control, Biological / Q000379:methods*; D016133:Polymerase Chain Reaction; D011647:Puerto Rico; D012680:Sensitivity and Specificity; D013785:Thailand
D017931:DNA Primers; D004279:DNA, Viral; D004247:DNA