title: Detection of 1-O-malylglucose: pelargonidin 3-O-glucose-6''-O-malyltransferase activity in carnation (Dianthus caryophyllus).
abstract: Carnations have anthocyanins acylated with malate. Although anthocyanin acyltransferases have been reported in several plant species, anthocyanin malyltransferase (AMalT) activity in carnation has not been identified. Here, an acyl donor substance of AMalT, 1-O-beta-D-malylglucose, was extracted and partially purified from the petals of carnation. This was synthesized chemically to analyze AMalT activity in a crude extract from carnation. Changes in the AMalT activity showed close correlation to the accumulation of pelargonidin 3-malylglucoside (Pel 3-malGlc) during the development of red petals of carnation, but neither AMalT activity nor Pel 3-malGlc accumulation was detectable in roots, stems and leaves.
authors.affiliation: Department of Biotechnology and Life Science, Faculty of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Abe
authors.forename: Masayuki
authors.initials: M
authors.lastname: Tera
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Sasaki
authors.forename: Masachika
authors.initials: M
authors.lastname: Okamura
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Umemoto
authors.forename: Masaki
authors.initials: M
authors.lastname: Momose
authors.forename: Nobuo
authors.initials: N
authors.lastname: Kawahara
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamakura
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Goda
authors.forename: Kazuo
authors.initials: K
authors.lastname: Nagasawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ozeki
mesh_terms: D000217:Acyltransferases / Q000378:metabolism*; D000872:Anthocyanins / Q000378:metabolism*; D029749:Dianthus / Q000201:enzymology* / Q000254:growth & development; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008293:Malates / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*
chemical_list: C530861:1-O-malylglucose; D000872:Anthocyanins; D005960:Glucosides; D008293:Malates; D010940:Plant Proteins; C530860:pelargonidin 3-malylglucoside; D000217:Acyltransferases