Synthesis of polynucleotide analogs containing a polyvinyl alcohol backbone.
Water soluble homo-base polynucleotide analogues were synthesized in which polyvinyl alcohol and partially phosphonated polyvinyl alcohol constituted the backbones,onto which were grafted uracil or adenine via 1,3-dioxane spacers formed by acetal formation with the 1,3-diol moieties in PVA. The resulting adenine-PVA polynucleotide analogs exhibited hyperchromic effects, which was not the case for the corresponding uracil compounds. Mixtures of the adenine- and aracil PVA-phosphate polynucleotide analogs in solutions exhibited characteristic S-shaped UV-absorbance vs temperature and melting curves with melting points at approximately 40 degrees C.
Yu
Qiang
Q
Department of Chemistry, Norwegian University of Science and Technology-NTNU, N-7491 Trondheim, Norway.
Carlsen
Per
P
D000225:Adenine / Q000737:chemistry; D010710:Phosphates / Q000737:chemistry; D011119:Polynucleotides / Q000138:chemical synthesis* / Q000737:chemistry; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D012995:Solubility; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D014498:Uracil / Q000737:chemistry
D010710:Phosphates; D011119:Polynucleotides; D014498:Uracil; D011142:Polyvinyl Alcohol; D000225:Adenine