Monte-Carlo-based model for the extraction of intrinsic fluorescence from turbid media.
A Monte-Carlo-based model of fluorescence is developed that is capable of extracting the intrinsic fluorescence properties of tissue, which are independent of the absorption and scattering properties of tissue. This model is flexible in its applicability to different illumination-collection geometries and is also valid for a wide range of optical properties, representative of tissue in the UV-visible spectrum. This is potentially useful in a variety of biomedical applications, including cancer diagnostics and monitoring the physiological response to therapy. The model is validated using phantoms composed of hemoglobin (absorber), polystyrene spheres (scatterer), and furan-2 (fluorophore). It is found that this model is able to retrieve the intrinsic fluorescence spectra of the tissue phantoms and recover the intrinsic fluorescence intensity of furan within the phantoms to within a mean error of less than 10%.
Palmer
Gregory M
GM
Duke University, Department of Radiation Oncology, Box 3455, Durham, North Carolina 27710, USA.
Ramanujam
Nirmala
N
D003102:Colloids / Q000737:chemistry*; D003198:Computer Simulation; D008954:Models, Biological; D008956:Models, Chemical; D015233:Models, Statistical; D009010:Monte Carlo Method; D009391:Nephelometry and Turbidimetry / Q000379:methods*; D013050:Spectrometry, Fluorescence / Q000379:methods*
D003102:Colloids