Region and age dependent differences in alpha(1)-adrenergic responsiveness of rat seminal vesicle and vas deferens.
Because age dependent differences occur in the incidence of ejaculatory dysfunction with alpha(1)-adrenoceptor antagonists used to treat lower urinary tract symptoms secondary to benign prostatic hyperplasia, we investigated age related changes in the functional, biochemical and molecular properties of alpha(1)-adrenoceptor in the rat seminal vesicle and vas deferens. The characteristics of alpha(1)-adrenoceptor in the seminal vesicle and epididymal and prostatic portion of vas deferens of 3 and 22-month-old rats were determined using an isolated muscle bath, radioligand receptor binding and real-time reverse transcription-polymerase chain reaction techniques. Old rats had significantly higher body weight and lower testosterone than young rats. Although there was no significant age dependent difference in the properties of alpha(1)-adrenoceptor in the prostatic portion of vas deferens, the maximum contractile responses to phenylephrine, total alpha(1)-adrenoceptor density and mRNA expression of all 3 alpha(1)-adrenoceptor subtypes were significantly lower in the seminal vesicle and epididymal portion of vas deferens of 22 vs 3-month-old rats. Age dependent differences in the molecular, biochemical and functional properties of alpha(1)-adrenoceptors in the rat seminal vesicle and vas deferens may indicate potential differences in the response to alpha(1)-adrenoceptor antagonists with aging.
Yono
Makoto
M
Department of Urology, Graduate School of Medical Sciences, Kumamoto University, 1-1-1 Honjo, Kumamoto 860-8556, Japan. yonomakoto@hotmail.com
Latifpour
Jamshid
J
Yamamoto
Yasuhiro
Y
Imanishi
Aya
A
Yoshida
Masaki
M
D000316:Adrenergic alpha-Agonists / Q000494:pharmacology; D000375:Aging / Q000502:physiology*; D000818:Animals; D001835:Body Weight / Q000502:physiology; D003627:Data Interpretation, Statistical; D066298:In Vitro Techniques; D008297:Male; D009119:Muscle Contraction / Q000187:drug effects / Q000502:physiology; D009130:Muscle, Smooth / Q000187:drug effects / Q000502:physiology; D010656:Phenylephrine / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011869:Radioligand Assay; D051381:Rats; D011916:Rats, Inbred F344; D018340:Receptors, Adrenergic, alpha-1 / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012669:Seminal Vesicles / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*; D013739:Testosterone / Q000097:blood; D014649:Vas Deferens / Q000033:anatomy & histology / Q000378:metabolism / Q000502:physiology*
D000316:Adrenergic alpha-Agonists; D018340:Receptors, Adrenergic, alpha-1; D010656:Phenylephrine; D013739:Testosterone; D011189:Potassium Chloride