title: Periostin and bone marrow fibrosis.
abstract: Periostin is a secreted protein that shares structural homology with the insect axon guidance protein fasciclin 1. Periostin is expressed predominantly in collagen-rich fibrous connective tissues that are subjected to constant mechanical stresses. We have shown previously that periostin is a novel component of subepithelial fibrosis in bronchial asthma. Here, we investigated the relationship between periostin and bone marrow (BM) fibrosis. Periostin was expressed in the stroma and stromal cells of BM fibrosis specimens and to a great extent its expression levels correlated closely to the grade of fibrosis, as estimated by silver staining. However, in the present study, we found no relationship between plasma periostin levels and the extent of BM fibrosis. We also demonstrated that periostin is secreted by human BM hTERT stromal cells and that its secretion is enhanced by TGF-beta, a cytokine produced by clonal proliferation of megakaryocytes and/or monocytes. These results indicate that periostin is a component of BM fibrosis and that it may play a role in the disease progression.
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Eijiro
authors.initials: E
authors.lastname: Oku
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan. kanajit@med.kurume-u.ac.jp.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Kanaji
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Yuka
authors.initials: Y
authors.lastname: Takata
authors.affiliation: Department of Pathology, Kurume University School of Medicine, Kurume, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Oshima
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Ritsuko
authors.initials: R
authors.lastname: Seki
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Morishige
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Rie
authors.initials: R
authors.lastname: Imamura
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Korenori
authors.initials: K
authors.lastname: Ohtsubo
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Michitoshi
authors.initials: M
authors.lastname: Hashiguchi
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Osaki
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yakushiji
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Yoshimoto
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ogata
authors.affiliation: Department of Molecular Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Hamada
authors.affiliation: Division of Medical Biochemistry, Department of Biomolecular Sciences, Saga Medical School, Saga, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Izuhara
authors.affiliation: Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, Kurume, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Sata
authors.affiliation: Division of Haematology, Kurume University School of Medicine, 67 Asahi-machi, Kurume, Fukuoka, 830-0011, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Okamura
mesh_terms: D000368:Aged; D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D008297:Male; D008533:Megakaryocytes / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism / Q000473:pathology; D055728:Primary Myelofibrosis / Q000378:metabolism* / Q000473:pathology; D017154:Stromal Cells / Q000378:metabolism / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000096:biosynthesis
chemical_list: D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D016212:Transforming Growth Factor beta