title: Development of hybrid polymer scaffolds for potential applications in ligament and tendon tissue engineering.
abstract: Fibre-based scaffolds have been widely used for tendon and ligament tissue engineering. Knitted scaffolds have been proved to favour collagenous matrix deposition which is crucial for tendon/ligament reconstruction. However, such scaffolds have the limitation of being dependent on a gel system for cell seeding, which is unstable in a dynamic environment such as the knee joint. This study developed three types of hybrid scaffolds, based on knitted biodegradable polyester scaffolds, aiming to improve mechanical properties and cell attachment and proliferation on the scaffolds. The hybrid scaffolds were created by coating the knitted scaffolds with a thin film of poly (epsilon-caprolactone) (group I), poly (D, L-lactide-co-glycolide) nanofibres (group II) and type 1 collagen (group III). Woven scaffolds were also fabricated and compared with the various hybrid scaffolds in terms of their mechanical properties during in vitro degradation and cell attachment and growth. This study demonstrated that the coating techniques could modulate the mechanical properties and facilitate cell attachment and proliferation in the hybrid scaffold, which could be applied with promise in tissue engineering of tendons/ligaments.
authors.affiliation: Tissue Repair Lab, Division of Bioengineering, National University of Singapore, Singapore. sambit@nus.edu.sg
authors.forename: Sambit
authors.initials: S
authors.lastname: Sahoo
authors.forename: James Goh
authors.initials: JG
authors.lastname: Cho-Hong
authors.forename: Toh
authors.initials: T
authors.lastname: Siew-Lok
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002478:Cells, Cultured; D024042:Collagen Type I / Q000737:chemistry*; D019245:Compressive Strength; D004548:Elasticity; D005240:Feasibility Studies; D019344:Lactic Acid / Q000737:chemistry*; D008022:Ligaments / Q000166:cytology / Q000254:growth & development*; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000502:physiology; D011091:Polyesters / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D013552:Swine; D013710:Tendons / Q000166:cytology / Q000254:growth & development*; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D024042:Collagen Type I; D011091:Polyesters; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C016240:polycaprolactone; D011100:Polyglycolic Acid; D019344:Lactic Acid