Delivery of a DNAzyme targeting c-myc to HT29 colon carcinoma cells using a gold nanoparticulate approach.
The objective of the current study was to develop cellular delivery approaches for catalytic DNA enzymes (DNAzymes) which cleave targeted messenger RNA, using vectors based on colloidal gold. The model DNAzyme was a 32mer oligonucleotide designed to specifically interact with and cleave c-myc mRNA. Colloidal gold particles were prepared by reduction of tetrachlororauric [III] acid with sodium citrate. Particles could be produced in the 1-90 nm range. A cationic substrate linked to transferrin was electrostatically/hydrophobically bound to the gold particle. These vectors were then treated with the DNAzyme to yield the condensed DNA-cationic polymer-particulate product. The pH (4-11.5), the quantity of the DNAzymes (0.079-0.567 microg/probe), the cationic polymer (polylysine (PL) or polyethylenimine (PEI)) as well as the surfactant (PVP) concentration (0-0.5%) were varied to give stable constructs which decomplexed under the desired conditions (i.e., in lysosomes and at lower pH values). Cellular uptake of the FITC-labelled c-myc DNAzyme incorporated in this vector was measured using FACS analysis in human HT29 colon carcinoma cells. Data suggested that PEI gave better delivery efficiencies than PL. The use of PVP to stabilize the formed dispersions was detrimental to DNAzyme delivery when PL was used but had little effect in the PEI systems. In the best cases, delivery to 77% of the cells was possible using PEI with the PVP stabilizer and completing the DNA condensation at pH 5.5 with 0.118 microg of DNAzyme/probe. In contrast, the best conditions for PL gave only transfection to 43% of the cells (no PVP, condensed at pH 5.7 and with a loading of 0.079 microg DNAzyme/probe). The PL probe tended to be more toxic than the PEI-based systems (65% cell death in PL transfected cells compared to 22% for PEI). These results suggest that cellular targeting using colloidal gold appears feasible for DNAzyme delivery.
Tack
F
F
Department of Molecular Cell Biology, Research Institute of Growth and Development, University of Maastricht, Maastricht, The Netherlands.
Noppe
M
M
Van Dijck
A
A
Dekeyzer
N
N
Van Der Leede
B J
BJ
Bakker
A
A
Wouters
W
W
Janicot
M
M
Brewster
M E
ME
D021881:DNA, Catalytic / Q000008:administration & dosage* / Q000494:pharmacology*; D004337:Drug Carriers; D016503:Drug Delivery Systems; D004355:Drug Stability; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate; D005456:Fluorescent Dyes; D017979:Gold Colloid / Q000008:administration & dosage* / Q000737:chemistry; D019073:HT29 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D016271:Proto-Oncogene Proteins c-myc / Q000187:drug effects*; D014162:Transfection
D021881:DNA, Catalytic; D004337:Drug Carriers; D005456:Fluorescent Dyes; D017979:Gold Colloid; D016271:Proto-Oncogene Proteins c-myc; D016650:Fluorescein-5-isothiocyanate