title: Synthesis, structural characteristics, DNA binding properties and cytotoxicity studies of a series of Ru(III) complexes.
abstract: Four related ruthenium(III) complexes, with the formula mer-[RuCl(3)(dmso)(N-N)] (dmso=dimethyl sulfoxide; N-N=2,2'-bipyridine (1), 1,10-phenantroline (2), dipyrido[3,2-f:2',3'-h]quinoxaline (3) and dipyrido[3,2-a:2',3'-c]phenazine (4)), have been reported. Complexes 3 and 4 are newly synthesized and characterized by X-ray diffraction. The hydrolysis process of 1-4 has been studied by UV-vis measurement, and it has been found that the extension of the N-N ligands can increase the stability of the complexes. The binding of these complexes with DNA has been investigated by plasmid cleavage assay, competitive binding with ethidium bromide (EB), DNA melting experiments and viscosity measurements. The DNA binding affinity is increased with the extension of the planar area of the N-N ligands, and complex 4 shows an intercalative mode of interaction with DNA. The in vitro anticancer activities of these compounds are moderate on the five human cancer cell lines screened.
authors.affiliation: MOE Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Xingang Road, Guangzhou 510275, PR China.
authors.forename: Caiping
authors.initials: C
authors.lastname: Tan
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.forename: Lanmei
authors.initials: L
authors.lastname: Chen
authors.forename: Shuo
authors.initials: S
authors.lastname: Shi
authors.forename: Liangnian
authors.initials: L
authors.lastname: Ji
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D001665:Binding Sites; D001667:Binding, Competitive; D016923:Cell Death; D045744:Cell Line, Tumor; D004247:DNA / Q000737:chemistry*; D004355:Drug Stability; D006801:Humans; D008024:Ligands; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D012428:Ruthenium; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D008024:Ligands; D009942:Organometallic Compounds; D012428:Ruthenium; D004247:DNA