title: Identifying the cellular basis for reimplantation failure in repair of the rotator cuff.
abstract: We examined cultured osteoblasts derived from paired samples from the greater tuberosity and acromion from eight patients with large chronic tears of the rotator cuff. We found that osteoblasts from the tuberosity had no apparent response to mechanical stimulation, whereas those derived from the acromion showed an increase in alkaline phosphatase activity and nitric oxide release which is normally a response of bone cells to mechanical strain. By contrast, we found that cells from both regions were able to respond to dexamethasone, a well-established promoter of osteoblastic differentiation, with the expected increase in alkaline phosphatase activity. Our findings indicate that the failure of repair of the rotator cuff may be due, at least in part, to a compromised capacity for mechanoadaptation within the greater tuberosity. It remains to be seen whether this apparent decrease in the sensitivity of bone cells to mechanical stimulation is the specific consequence of the reduced load-bearing history of the greater tuberosity in these patients.
authors.affiliation: Department of Trauma and Orthopaedic Surgery King's College Hospital, Denmark Hill, London SE59RS, UK. domsimon@doctors.org.uk
authors.forename: D W N
authors.initials: DW
authors.lastname: Simon
authors.forename: C E
authors.initials: CE
authors.lastname: Clarkin
authors.forename: V
authors.initials: V
authors.lastname: Das-Gupta
authors.forename: S C F
authors.initials: SC
authors.lastname: Rawlinson
authors.forename: R J
authors.initials: RJ
authors.lastname: Emery
authors.forename: A A
authors.initials: AA
authors.lastname: Pitsillides
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000469:Alkaline Phosphatase / Q000378:metabolism; D001842:Bone and Bones / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000378:metabolism; D010006:Osteoblasts / Q000378:metabolism*; D012092:Replantation; D017006:Rotator Cuff / Q000601:surgery*; D000070636:Rotator Cuff Injuries; D012785:Shoulder Joint / Q000503:physiopathology / Q000601:surgery*; D013223:Statistics as Topic; D016474:Weight-Bearing; D014945:Wound Healing / Q000502:physiology
chemical_list: D009569:Nitric Oxide; D000469:Alkaline Phosphatase