title: Application of restriction fragment length polymorphism analysis to simple and rapid genotyping of bovine viral diarrhea virus strains isolated in Japan.
abstract: The E2 regions of 177 bovine viral diarrhea virus (BVDV) strains isolated in Japan between 1957 and 2006 were analyzed for genotyping. The strains were classified into 8 genotypes (1a, 1b, 1c, 1d, 1e, 1f, So and 2a) based on the phylogenetic analysis. The restriction fragment length polymorphism (RFLP) analysis of the RT-PCR products using 6 selected enzymes (Apo I, Mly I, BstAP I, Pvu II, Ear I, EcoR V) disclosed the cutting patterns classified into 11 groups (I-XI), each of that consisted of strains belonging to a single genotype. Namely, groups-I and -II were composed by genotype-1a strains, groups-III and -IV by 1b strains, and groups-V and -VI by 1c strains. Other groups-VII, -VIII, -IX, -X and -XI comprised genotypes-1d, -1e, -1f, -So and -2a strains, respectively. The results suggest that the RFLP analysis can simply and rapidly differentiate the 8 genotypes of BVDV strains.
authors.affiliation: Iwate Prefecture Central Livestock Hygiene Service Center, Takizawa-mura, Iwate, Japan. yoshihisa-seki@pref.iwate.jp
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Seki
authors.forename: Yukio M
authors.initials: YM
authors.lastname: Seimiya
authors.forename: Masato
authors.initials: M
authors.lastname: Motokawa
authors.forename: Gakuji
authors.initials: G
authors.lastname: Yaegashi
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagai
authors.forename: Michiko
authors.initials: M
authors.lastname: Hayashi
mesh_terms: D000818:Animals; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification* / Q000235:genetics*; D005838:Genotype; D007564:Japan; D016133:Polymerase Chain Reaction / Q000662:veterinary; D012150:Polymorphism, Restriction Fragment Length