Preparation of scaffolds from human hair proteins for tissue-engineering applications.
Human hair proteins were isolated and purified for the fabrication of tissue-engineering scaffolds. Their cellular compatibility was studied using NIH3T3 mice fibroblast cells. The proteins were characterized using FTIR spectroscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis for molecular weights and two-dimensional polyacrylamide gel electrophoresis for their isoelectric points (pIs). The molecular weights of keratins were in the range of 40-60 kilo-Daltons (kDa) and of matrix proteins were in the range of 15-30 kDa. The pIs of keratins were found to be in the range of 4.5-5.3. Sponges of the proteins were formed by lyophilization. Scanning electron microscopy was performed to examine the surface. Swelling studies were carried out in phosphate buffer saline at physiological pH 7.4. The hydrophilic character of the protein surface was studied by determining an average contact angle, which came to be 37 degrees. The wells of tissue culture plates were coated with these proteins for studying the attachment and morphology of the cells. The protein detachment study was done to ensure the adsorption of proteins on the wells until the completion of the experiments. The cellular growth on a protein-coated surface showed three-dimensional 'bulged' morphology due to cell-cell and cell-matrix contacts. The sponges of human hair proteins supported more cells for a longer period than control. The morphology and cell proliferation studies exhibited by NIH3T3 cells on these proteins have shown their potential to be used as tissue-engineering scaffolds with better cell-cell contacts and leucine-aspartic acid-valine (LDV)-mediated cell-matrix interactions.
Verma
Vipin
V
Centre for Biomedical Engineering, Indian Institute of Technology Delhi (IITD), New Delhi-110 016, India.
Verma
Poonam
P
Ray
Pratima
P
Ray
Alok R
AR
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D048429:Cell Size; D016326:Extracellular Matrix Proteins / Q000737:chemistry* / Q000378:metabolism; D006197:Hair / Q000378:metabolism*; D006801:Humans; D007633:Keratins / Q000737:chemistry* / Q000378:metabolism; D008422:Materials Testing; D051379:Mice; D008970:Molecular Weight; D041681:NIH 3T3 Cells; D011485:Protein Binding; D023822:Tissue Engineering / Q000379:methods*
D001672:Biocompatible Materials; D016326:Extracellular Matrix Proteins; D007633:Keratins