The effects of increasing sodium chloride concentration on Mycoplasma gallisepticum vaccine survival in solution.
Lyophilized Mycoplasma gallisepticum (MG) vaccines are generally rehydrated and diluted with distilled or chlorine-free water as per manufacturer recommendations. However, as mycoplasma species lack a cell wall, this can lead to decreased viability of live vaccine during administration. The ability of phosphate-buffered saline (PBS) to prevent losses in live vaccine viability was examined. It was shown that a concentration of 1 x PBS prevented the two-fourfold decrease in MG viability seen when the vaccines were diluted with water alone.
Leigh
S A
SA
USDA, ARS South Central Poultry Research Unit, Mississippi State, MS 39762, USA. sleigh@msa-msstate.ars.usda.gov
Evans
J D
JD
Branton
S L
SL
Collier
S D
SD
D001428:Bacterial Vaccines; D004355:Drug Stability; D005612:Freeze Drying; D045686:Mycoplasma gallisepticum / Q000187:drug effects* / Q000276:immunology*; D012965:Sodium Chloride / Q000494:pharmacology*
D001428:Bacterial Vaccines; D012965:Sodium Chloride