title: Highly effective cell synchronization in plant roots by hydroxyurea and amiprophos-methyl or colchicine.
abstract: Effective somatic cell synchronization in root-tip meristems and improved chromosome spreading were achieved in white campion, wheat, rye, and barley by application of hydroxyurea and amiprophos-methyl or colchicine, combined with a pretreatment of ice water and modified fixative, as well as enzymatic digestion of the meristems. The protocol provides metaphase indices of approximately 50%. The chromosomes and chromosomal DNA were with minimum distortion, providing useful material for chromosome banding studies, in situ DNA-DNA hybridization, microdissection, and microcloning.
authors.forename: W H
authors.initials: WH
authors.lastname: Pan
authors.forename: A
authors.initials: A
authors.lastname: Houben
authors.forename: R
authors.initials: R
authors.lastname: Schlegel