title: Effects of sulfonylurea drugs on adiponectin production from 3T3-L1 adipocytes: implication of different mechanism from pioglitazone.
abstract: Adiponectin is a fat-derived cytokine with anti-diabetic and anti-atherogenic properties. In this study, effects of sulfonylureas (SUs) on adiponectin production and the action mechanism were evaluated using 3T3-L1 adipocytes. The cells were incubated with glimepiride, glibenclamide, gliclazide, pioglitazone, metformin and the medium only as the control. In the control, the adiponectin level evaluated as the production rate per 24 h was not changed, while pioglitazone significantly increased the adiponectin level. SUs also increased the adiponectin level, but metformin failed to show any increase in adiponectin production. SUs induced adiponectin gene expression as well as pioglitazone. Pioglitazone significantly increased adipogenesis, but glimepiride did not. The aP2 gene expression was increased by pioglitazone, but not by glimepiride. Forskolin, a protein kinase A stimulator, reduced the adiponectin production stimulated by glimepiride but not by pioglitazone. These observations strongly suggest that SUs stimulate the adiponectin production through a different mechanism from pioglitazone, namely an interaction with protein kinase A activity. The significance of the extrapancreatic action of SUs observed in this study should be further evaluated in the clinical field.
authors.affiliation: Diabetes and Endocrine Division, Department of Medicine, Kawasaki Medical School, 577 Matsushima, Kurashiki-shi, Okayama-ken 701-0192, Japan.
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Kanda
authors.forename: Masafumi
authors.initials: M
authors.lastname: Matsuda
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Tawaramoto
authors.forename: Fumiko
authors.initials: F
authors.lastname: Kawasaki
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Hashiramoto
authors.forename: Michihiro
authors.initials: M
authors.lastname: Matsuki
authors.forename: Kohei
authors.initials: K
authors.lastname: Kaku
mesh_terms: D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000502:physiology*; D052242:Adiponectin / Q000096:biosynthesis*; D000818:Animals; D051379:Mice; D000077205:Pioglitazone; D013453:Sulfonylurea Compounds / Q000494:pharmacology*; D045162:Thiazolidinediones / Q000494:pharmacology*
chemical_list: D052242:Adiponectin; D013453:Sulfonylurea Compounds; D045162:Thiazolidinediones; D000077205:Pioglitazone