title: Transgenic sheep designed for transplantation studies.
abstract: Lentiviral vectors have recently emerged as an efficient method of transgene delivery to the germline of animals. We now demonstrate that combining this efficiency with embryo splitting procedures enables the production of monozygotic twins, one of which is transgenic. We propose that this approach can be used to generate animals in which cell or tissue transplantation can be achieved without the use of immunosuppressive regimes.
authors.affiliation: Roslin Institute, Animal Services Department, Roslin, Midlothian, UK.
authors.forename: William A
authors.initials: WA
authors.lastname: Ritchie
authors.forename: Tim
authors.initials: T
authors.lastname: King
authors.forename: Claire
authors.initials: C
authors.lastname: Neil
authors.forename: Ailsa J
authors.initials: AJ
authors.lastname: Carlisle
authors.forename: Simon
authors.initials: S
authors.lastname: Lillico
authors.forename: Gerry
authors.initials: G
authors.lastname: McLachlan
authors.forename: C Bruce A
authors.initials: CB
authors.lastname: Whitelaw
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000378:metabolism*; D017690:Cell Transplantation / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics; D016086:Lentivirus / Q000235:genetics; D012756:Sheep / Q000235:genetics* / Q000378:metabolism*; D016378:Tissue Transplantation / Q000379:methods*; D019076:Transgenes / Q000235:genetics