title: Genotypic resistance profile of HIV-1 protease gene: a preliminary report from Vellore, south India.
abstract: HIV-1 subtypes other than B are responsible for most new HIV infections worldwide; virus sequence data for drug resistance is described only from a limited number of non-B subtype HIV-1. This study is on mutations and polymorphisms of HIV-1 protease gene that can predict drug resistance in subtype C. The genotypic resistance assay was carried out on 38 HIV-1 strains with their plasma RNA and in nine, the proviral protease gene was sequenced. The treatment naïve strains showed minor resistance mutations, there were no major resistance mutations in the protease gene. We suggest the use of resistance testing to monitor individuals on therapy and also before initiation of therapy, gathering more sequence information for a data bank of Indian strains.
authors.affiliation: Department of Clinical Virology, Christian Medical College, Vellore-632 004, Tamil Nadu, India.
authors.forename: A J
authors.initials: AJ
authors.lastname: Kandathil
authors.forename: R
authors.initials: R
authors.lastname: Kannangai
authors.forename: O C
authors.initials: OC
authors.lastname: Abraham
authors.forename: T D
authors.initials: TD
authors.lastname: Sudarsanam
authors.forename: S A
authors.initials: SA
authors.lastname: Pulimood
authors.forename: G
authors.initials: G
authors.lastname: Sridharan
mesh_terms: D019943:Amino Acid Substitution / Q000235:genetics; D024882:Drug Resistance, Viral / Q000235:genetics*; D005838:Genotype; D015658:HIV Infections / Q000821:virology*; D016333:HIV Protease / Q000235:genetics*; D015497:HIV-1 / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D007194:India; D020125:Mutation, Missense; D012367:RNA, Viral / Q000097:blood / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA
chemical_list: D012367:RNA, Viral; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1