title: Infection with Toxoplasma gondii does not Alter TNFalpha and IL-6 Secretion by A human Astrocytoma Cell Line.
abstract: The secretion of tumour necrosis factor-alpha (TNFalpha), interleukin-1alpha (IL-alpha) and interleukin-6 (IL-6) by a human astrocytoma cell fine was studied 1 h, 3 h, 6 h and 24 h after infection with tachyzoites from three Toxoplasma gondii strains (virulent, RH; cystogentc, 76K and Prugniaud strains). The astrocytoma cell fine constitutively secreted TNFalpha and IL-6, but no IL-1alpha. A positive control was obtained by stimulation with phorbol esters inducing a significant increase (p < 0.05) in TNFalpha and IL- 6 secretion but not in IL-1alpha, while lipopolysaccharide (alone and after priming), interferon gamma, ionophore A 23187 and sera positive to T. gondii did not induce any increase in cytokine levels. None of the tachyzoites, whatever their virulence, induced a significant increase in cytokine production at any time in the study. Tachyzoites did not inhibit the secretion induced by phorbol esters.
authors.affiliation: Dédpartement de Parasitologie-Mycologie Médicale et Moléculaire CNRS ERS 15, Faculté de Médecine Université Joseph Fourier Grenoble 1, Grenoble Cedex 38043 France.
authors.forename: H
authors.initials: H
authors.lastname: Pelloux
authors.forename: J
authors.initials: J
authors.lastname: Ricard
authors.forename: M F
authors.initials: MF
authors.lastname: Nissou
authors.forename: J C
authors.initials: JC
authors.lastname: Renversez
authors.forename: J P
authors.initials: JP
authors.lastname: Vuillez
authors.forename: A
authors.initials: A
authors.lastname: Meunier
authors.forename: P
authors.initials: P
authors.lastname: Ambroise-Thomas