Aeromonas aquariorum sp. nov., isolated from aquaria of ornamental fish.
During a survey to determine the prevalence of Aeromonas strains in water and skin of imported ornamental fish, 48 strains presumptively identified as Aeromonas were isolated but they could not be identified as members of any previously described Aeromonas species. These strains were subjected to a polyphasic approach including phylogenetic analysis derived from gyrB, rpoD and 16S rRNA gene sequencing, DNA-DNA hybridization, MALDI-TOF MS analysis, genotyping by RAPD and extensive biochemical and antibiotic susceptibility tests in order to determine their taxonomic position. Based on the results of the phylogenetic analyses and DNA-DNA hybridization data, we describe a novel species of the genus Aeromonas, for which the name Aeromonas aquariorum sp. nov. is proposed, with strain MDC47T (=DSM 18362T =CECT 7289T) as the type strain. This is the first Aeromonas species description based on isolations from ornamental fish.
Martínez-Murcia
A J
AJ
Molecular Diagnostics Center, Biomolecular Technologies S.L. and Universidad Miguel Hernández, E-03300 Orihuela (Alicante), Spain. ammurcia@mdc-bt.com
Saavedra
M J
MJ
Mota
V R
VR
Maier
T
T
Stackebrandt
E
E
Cousin
S
S
D000333:Aeromonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D017756:Aquaculture; D015373:Bacterial Typing Techniques; D027081:DNA Gyrase / Q000235:genetics; D004269:DNA, Bacterial / Q000032:analysis; D012321:DNA-Directed RNA Polymerases / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology*; D005399:Fishes / Q000382:microbiology; D020459:Genes, rRNA; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology / Q000662:veterinary*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D019105:Random Amplified Polymorphic DNA Technique; D017422:Sequence Analysis, DNA; D012808:Sigma Factor / Q000235:genetics; D012867:Skin / Q000382:microbiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014871:Water Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases; D027081:DNA Gyrase