An experimental test of stroke recovery by implanting a hyaluronic acid hydrogel carrying a Nogo receptor antibody in a rat model.
The objective of the study was to determine the effects of a hyaluronic-acid-based (HA-based) hydrogel implant, carrying a polyclonal antibody to the Nogo-66 receptor (NgR), on adult rats that underwent middle cerebral artery occlusion (MCAO). Behavioral tests of a forelimb-reaching task suggested that the disabled function of the impaired forelimb in this stroke model was ameliorated by the implant to a certain extent. These behavioral findings were correlated with immunohistochemical results of investigating the distribution of NgR antibody, neurofilaments (NF) and neuron-specific class III beta-tubulin (TuJ1) in the brain sections. The porous hydrogel functioned as a scaffold to deliver the NgR antibody, support cell migration and development. In addition, it was found NF-positive and TuJ1-positive expressions were distributed in the implanted hydrogel. Collectively, the results demonstrate the promise of the HA hydrogel as a scaffold material and the delivery vehicle of the NgR antibody for the repair of defects and the support of neural regeneration in the brain.
Ma
Jun
J
Biomaterials Laboratory, Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
Tian
Wei-Ming
WM
Hou
Shao-Ping
SP
Xu
Qun-Yuan
QY
Spector
Myron
M
Cui
Fu-Zhai
FZ
D000818:Animals; D000911:Antibodies, Monoclonal / Q000008:administration & dosage* / Q000737:chemistry; D004195:Disease Models, Animal; D004343:Drug Implants / Q000737:chemistry*; D058851:GPI-Linked Proteins; D006820:Hyaluronic Acid / Q000737:chemistry*; D020100:Hydrogels / Q000737:chemistry; D009185:Myelin Proteins / Q000276:immunology*; D000070816:Nogo Receptor 1; D010291:Paresis / Q000188:drug therapy* / Q000209:etiology; D051381:Rats; D011956:Receptors, Cell Surface / Q000276:immunology*; D020127:Recovery of Function / Q000187:drug effects*; D020521:Stroke / Q000150:complications / Q000188:drug therapy*; D016896:Treatment Outcome
D000911:Antibodies, Monoclonal; D004343:Drug Implants; D058851:GPI-Linked Proteins; D020100:Hydrogels; D009185:Myelin Proteins; D000070816:Nogo Receptor 1; C486580:RTN4R protein, human; D011956:Receptors, Cell Surface; D006820:Hyaluronic Acid