title: Association of cholesteryl ester transfer protein (TaqIB) and apolipoprotein E (HhaI) gene variants with obesity.
abstract: Background The pathophysiology of obesity is known to be influenced by alterations in lipid levels. We aimed to evaluate association of cholesteryl ester transfer protein (CETP) and apolipoprotein (APO) E gene variants with asymptomatic obesity. Methods A total of 437 subjects, 159 asymptomatic obese (BMI = 29.29 +/- 3.76) and 278 non-obese (BMI = 23.38 +/- 1.71) individuals, were included in this case-control study. Lipid levels were estimated using standard protocols. Analysis of CETP (TaqIB) and APOE (HhaI) gene polymorphisms was done using PCR-RFLP. Results We found significant difference in blood pressure (systolic, P < 0.0001 and diastolic, P < 0.0001), total cholesterol (P < 0.0001), LDL-cholesterol (P < 0.0001), and HDL-cholesterol (P < 0.0001) in obese as compared to non-obese group. Homozygous APO E4E4 genotype was only observed in 5.7% of obese individuals and none in non-obese group. APO E4 allele carriers were also susceptible for obesity (P = 0.016, OR = 1.73; 95% CI = 1.12-2.68) than non-carriers. Higher blood pressure (Systolic, P = 0.001 and Diastolic, P = 0.004) and triglyceride levels (P = 0.029) were observed in obese subjects with APO E4 allele than individuals without APO E4. However, CETP B1 variant allele carriers did not show alteration in blood pressure and lipid profile in asymptomatic obese subjects. Conclusions APO E4 genotype and allele were found to be associated with asymptomatic obesity, whereas CETP Taq1B polymorphism showed no such association in North Indian subjects.
authors.affiliation: Department of Physiology, Chatrapati Shahuji Maharaj Medical University, Lucknow, India.
authors.forename: Neena
authors.initials: N
authors.lastname: Srivastava
authors.forename: B R
authors.initials: BR
authors.lastname: Achyut
authors.forename: Jai
authors.initials: J
authors.lastname: Prakash
authors.forename: C G
authors.initials: CG
authors.lastname: Agarwal
authors.forename: D C
authors.initials: DC
authors.lastname: Pant
authors.forename: Balraj
authors.initials: B
authors.lastname: Mittal
mesh_terms: D001057:Apolipoproteins E / Q000235:genetics*; D001794:Blood Pressure / Q000235:genetics; D015992:Body Mass Index; D053480:Cholesterol Ester Transfer Proteins / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D005787:Gene Frequency; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D007194:India; D008055:Lipids / Q000097:blood; D009765:Obesity / Q000097:blood / Q000235:genetics* / Q000503:physiopathology; D012150:Polymorphism, Restriction Fragment Length
chemical_list: D001057:Apolipoproteins E; D053480:Cholesterol Ester Transfer Proteins; D008055:Lipids; D015252:Deoxyribonucleases, Type II Site-Specific; C037130:GCGC-specific type II deoxyribonucleases; C027046:TCGA-specific type II deoxyribonucleases