[An experimental study on biocompatibility of Bio-oss collagen with cultured bone marrow stromal cells].
OBJECTIVE
To study the biocompatibility of Bio-oss collagen with cultured bone marrow stromal cells.


METHODS
The bone marrow stromal cells (BMSCs) of rhesus monkey were cultured with Bio-oss collagen in vitro. Cell attachment was observed with laser confocal microscope. Cell proliferation rates were assessed with MTT assay. ALP activity was also detected. The data was statistically analyzed with SAS6.12 software package for Student's t test.


RESULTS
The rhesus BMSCs could attach to the surface of Bio-oss collagen. In cell proliferation rates, there was no significant difference between the control group and experimental group at 2-day and 5-day(P>0.05). However, at 8-day, the difference was significant (P<0.05). For ALP activity, there was no significant difference among different time point (P>0.05).


CONCLUSIONS
The Bio-oss collagen has good biocompatibility with rhesus BMSCs, and could be used to repair periodontal bone defects as a biomaterial in tissue engineering.
Song
Zhong-Chen
ZC
Department of Periodontology, College of Stomatology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Shu
Rong
R
Xie
Yu-Feng
YF
Zhang
Xiu-Li
XL
Yin
De-Min
DM
D000818:Animals; D001672:Biocompatible Materials; D003094:Collagen; D008253:Macaca mulatta; D059630:Mesenchymal Stem Cells; D008903:Minerals
C077540:Bio-Oss; D001672:Biocompatible Materials; D008903:Minerals; D003094:Collagen