Characterization of fluoroalcohols-induced intermediates of Mucor miehei lipase at low pH.
We have previously characterized an acid-unfolded (U(A)) state of Mucor miehei lipase at pH 2. The effect of 2,2,2-trifluoroethanol (TFE) and 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) resulted in characterization of molten-globule (MG) like states with beta-sheet secondary structure at 15% (v/v) TFE and 6% (v/v) HFIP. alpha-Helical states accumulate at 80% (v/v) TFE and 30% (v/v) HFIP.
Fatima
Sadaf
S
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India. rizwanhkhan@hotmail.com.
Mishra
Aseem
A
Sen
Priyankar
P
Khan
Rizwan Hasan
RH
D002942:Circular Dichroism; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry*; D009089:Mucor / Q000201:enzymology*; D020005:Propanols / Q000494:pharmacology*; D011487:Protein Conformation / Q000187:drug effects; D011489:Protein Denaturation; D017510:Protein Folding; D017433:Protein Structure, Secondary / Q000187:drug effects; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014270:Trifluoroethanol / Q000494:pharmacology*; D006845:Hydrocarbons, Fluorinated
D008049:Lipase; D020005:Propanols; D014270:Trifluoroethanol; C001337:hexafluoroisopropanol; D006845:Hydrocarbons, Fluorinated