title: [Effect of inoculation time on the survival of spores of Gliocladium roseum on geranium leaves.].
abstract: Gliocladium roseumis a successful antagonist of Botrytis cinerea and is considered to have the major potential for biocontrol of the pathogen in cropping systems. In order to elucidate the optimal moment of the day to apply the biological control agent, geranium plants were inoculated until run off with a suspension containing 10 e7 conidia of G. roseum + Tritón 100X. The inoculation times were 9 am, 12 am, 3 pm and 6 pm. The number cfu per cm(2) of leaves at inoculation time (time 0) and at 3, 6 y 9 h after inoculation were estimated. Then, the inoculated plants were kept in a Plexiglas, humidity chamber for 24 h. Discs of 8 mm in diameter were cut off from the inoculated leaves and put then into a paraquat medium to examine the development of G. roseum. According to the results dry period length after inoculation time, shows a significant decrease in number of cfu by cm(2) of leaf. No difference was observed between 3, 6 y 9 h after inoculation time.
authors.affiliation: Facultad de Ciencias Agrarias y Forestales, Laboratorio de Fitopatología, Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, Argentina. cmonaco@cadema.com.ar.
authors.forename: C
authors.initials: C
authors.lastname: Mónaco
authors.forename: H
authors.initials: H
authors.lastname: Yu
authors.forename: J
authors.initials: J
authors.lastname: Sutton