title: Enzymatic hydrolysis of penicillin for 6-APA production in three-liquid-phase system.
abstract: A dodecane/thermosensitive polymer/water three-liquid-phase system was introduced for enzymatic hydrolysis of penicillin G (Pen G) for 6-aminopenicillanic acid (6-APA). The enzyme was covalently attached to the terminal of PEO-PPO-PEO polymer (L63), which would be transferred into a polymer coacervate phase at high temperature above its "cloud point". 6-APA was primarily resided in the aqueous phase due to its zwitterionic nature. More than 70% phenylacetic acid (PAA) was transferred into the organic phase using trioctylmethylammonium hydroxide and trihexyl-(tetradecyl)phosphonium bis 2,4,4-trimethylpentylphosphinate ionic liquids (Cyphos IL-104) mixture at pH 5.5, while most of Pen G resided in water. As a result, high operational pH was permitted in three-liquid-phase system, which leads to higher enzymatic activity (120 IU at 40 degrees C) and stability (enzymatic half-time up to 55 h at 60 degrees C) in comparison with the value in butyl acetate/water two-phase system. On the other hand, two products in three-liquid-phase system might be automatically separated from the enzyme sphere into different phases at the same time, which facilitated the reaction equilibrium towards the product's side with 6-APA productivity of 80% at 42 degrees C, pH 5.5.
authors.affiliation: Laboratory of Separation Science and Engineering, Institute of Process Engineering, Chinese Academy of Science, Beijing 100080, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Jiang
authors.forename: Hansong
authors.initials: H
authors.lastname: Xia
authors.forename: Chen
authors.initials: C
authors.lastname: Guo
authors.forename: Iram
authors.initials: I
authors.lastname: Mahmood
authors.forename: Huizhou
authors.initials: H
authors.lastname: Liu
mesh_terms: D000473:Alkanes / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D006868:Hydrolysis; D052578:Ionic Liquids / Q000737:chemistry; D007700:Kinetics; D015394:Molecular Structure; D010397:Penicillanic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D010406:Penicillins / Q000737:chemistry / Q000378:metabolism*; D044367:Phase Transition; D011092:Polyethylene Glycols / Q000737:chemistry; D011409:Propylene Glycols / Q000737:chemistry
chemical_list: D000473:Alkanes; D052578:Ionic Liquids; C116176:PEO-PPO-PEO; D010406:Penicillins; D011409:Propylene Glycols; C007548:n-dodecane; D011092:Polyethylene Glycols; D010397:Penicillanic Acid; C004408:aminopenicillanic acid