title: The stimulatory activities of polysaccharide compounds derived from algae extracts on insulin secretion in vitro.
abstract: We prepared two series of polysaccharide compounds derived from algae extracts and investigated their stimulatory activity on insulin secretion in vitro using the rat pancreatic cell line, RIN-5F. Several of the compounds exhibited significant stimulatory activity in a dose-dependent manner without apparent cytotoxicity at concentrations above 10 microM. Glybenclamide, a commonly prescribed sulfonylurea (SU) against diabetes mellitus type II, was used as a positive control and showed moderate cytotoxicity in the cell culture assay system. Amylin (IAPP; islet amyloid polypeptide), an inhibitor for glybenclamide, did not inhibit the activity of the isolated compounds, suggesting that they act through a mechanism(s) different from glybenclamide. Algae-derived extracts could be candidates for a new class of anti-diabetic drugs.
authors.affiliation: The Institute of Marine Drug and Food, College of Medicine and Pharmaceutics, Ocean University of China, 5 Yushan Road, Qingdao 266003, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhang
authors.forename: Isao
authors.initials: I
authors.lastname: Fujii
authors.forename: Changzheng
authors.initials: C
authors.lastname: Lin
authors.forename: Kaori
authors.initials: K
authors.lastname: Ito
authors.forename: Huashi
authors.initials: H
authors.lastname: Guan
authors.forename: Ji'en
authors.initials: J
authors.lastname: Zhao
authors.forename: Makoto
authors.initials: M
authors.lastname: Shinohara
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsukura
mesh_terms: D000818:Animals; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D056890:Eukaryota / Q000737:chemistry*; D005905:Glyburide / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007202:Indicators and Reagents; D007328:Insulin / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D007515:Islets of Langerhans / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D051381:Rats; D013268:Stimulation, Chemical
chemical_list: D007004:Hypoglycemic Agents; D007202:Indicators and Reagents; D007328:Insulin; D008070:Lipopolysaccharides; D005905:Glyburide