title: A live attenuated severe acute respiratory syndrome coronavirus is immunogenic and efficacious in golden Syrian hamsters.
abstract: The immunogenicity and protective efficacy of a live attenuated vaccine consisting of a recombinant severe acute respiratory syndrome (SARS) coronavirus lacking the E gene (rSARS-CoV-DeltaE) were studied using hamsters. Hamsters immunized with rSARS-CoV-DeltaE developed high serum-neutralizing antibody titers and were protected from replication of homologous (SARS-CoV Urbani) and heterologous (GD03) SARS-CoV in the upper and lower respiratory tract. rSARS-CoV-DeltaE-immunized hamsters remained active following wild-type virus challenge, while mock-immunized hamsters displayed decreased activity. Despite being attenuated in replication in the respiratory tract, rSARS-CoV-DeltaE is an immunogenic and efficacious vaccine in hamsters.
authors.affiliation: Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA.
authors.forename: Elaine W
authors.initials: EW
authors.lastname: Lamirande
authors.forename: Marta L
authors.initials: ML
authors.lastname: DeDiego
authors.forename: Anjeanette
authors.initials: A
authors.lastname: Roberts
authors.forename: Jadon P
authors.initials: JP
authors.lastname: Jackson
authors.forename: Enrique
authors.initials: E
authors.lastname: Alvarez
authors.forename: Tim
authors.initials: T
authors.lastname: Sheahan
authors.forename: Wun-Ju
authors.initials: WJ
authors.lastname: Shieh
authors.forename: Sherif R
authors.initials: SR
authors.lastname: Zaki
authors.forename: Ralph
authors.initials: R
authors.lastname: Baric
authors.forename: Luis
authors.initials: L
authors.lastname: Enjuanes
authors.forename: Kanta
authors.initials: K
authors.lastname: Subbarao
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D006224:Cricetinae; D017353:Gene Deletion; D008168:Lung / Q000473:pathology / Q000821:virology; D008647:Mesocricetus; D009500:Neutralization Tests; D012137:Respiratory System / Q000821:virology; D045473:Severe acute respiratory syndrome-related coronavirus / Q000235:genetics / Q000276:immunology*; D045169:Severe Acute Respiratory Syndrome / Q000276:immunology / Q000517:prevention & control*; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins / Q000235:genetics; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology*; D000086082:Viroporin Proteins
chemical_list: D000914:Antibodies, Viral; C501689:E protein, SARS coronavirus; D014613:Vaccines, Attenuated; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; D000086082:Viroporin Proteins