title: Disturbance of pro-oxidative/antioxidative balance in allogeneic peripheral blood stem cell transplantation.
abstract: High dose chemotherapy causes increased free radical formation and depletion of tissue antioxidants. Whether allogeneic hematopoietic stem cell transplantation (HSCT) has an effect on oxidative stress is uncertain. The aims of the study were to determine the effect of allogeneic HSCT on plasma concentrations of antioxidants and oxidative stress biomarkers, and to investigate their relationships with graft-versus-host disease (GVHD), conditioning regimens, and transplant-related mortality (TRM) in patients with hematological malignancies. Patients (n=25) undergoing allogeneic HSCT from HLA-matched sibling donors were enrolled in the study. Plasma oxidant and antioxidant status were measured at day -1 before transplantation and 30 days after HSCT. In both myeloablative (n=14) and non-myeloablative (n=11) transplant groups, the mean levels of plasma malondialdehyde (MDA) and nitric oxide (NO) increased after allogeneic HSCT (p <0.01), whereas superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) activities decreased compared with baseline values (p <0.01). No significant relationships were found between either the pretransplant or post-transplant mean levels of the oxidative stress parameters and the existence of graft-versus-host disease (GVHD), the type of conditioning regimen, or transplant related mortality (TRM). This study documents a significant disturbance of pro-oxidative/antioxidative balance in the plasma of patients undergoing allogeneic HSCT regardless of the intensity of the conditioning regimen.
authors.affiliation: Department of Hematology, Pamukkale University Faculty of Medicine, TR-20070, Denizli, Turkey. hisari@pau.edu.tr <hisari@pau.edu.tr>
authors.forename: Ismail
authors.initials: I
authors.lastname: Sari
authors.forename: Aysun
authors.initials: A
authors.lastname: Cetin
authors.forename: Leylagul
authors.initials: L
authors.lastname: Kaynar
authors.forename: Recep
authors.initials: R
authors.lastname: Saraymen
authors.forename: Sibel Kabukcu
authors.initials: SK
authors.lastname: Hacioglu
authors.forename: Ahmet
authors.initials: A
authors.lastname: Ozturk
authors.forename: Ismail
authors.initials: I
authors.lastname: Kocyigit
authors.forename: Fevzi
authors.initials: F
authors.lastname: Altuntas
authors.forename: Bulent
authors.initials: B
authors.lastname: Eser
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood; D002374:Catalase / Q000097:blood; D005260:Female; D005979:Glutathione Peroxidase / Q000097:blood; D006086:Graft vs Host Disease / Q000097:blood; D006801:Humans; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D009569:Nitric Oxide / Q000097:blood; D016877:Oxidants / Q000097:blood*; D018384:Oxidative Stress; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects*; D013482:Superoxide Dismutase / Q000097:blood; D019172:Transplantation Conditioning; D014184:Transplantation, Homologous
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D016877:Oxidants; D009569:Nitric Oxide; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase