Typing of SHV extended-spectrum beta-lactamases by pyrosequencing in Klebsiella pneumoniae strains with chromosomal SHV beta-lactamase.
In Klebsiella pneumoniae, the cooccurrence of chromosomal and plasmid-mediated beta-lactamases can hinder their accurate molecular detection. We developed a fast and reliable method that allows the typing of isolates carrying more than one SHV gene. The method is based on pyrosequencing the DNA sequence corresponding to amino acid positions 35, 238, and 240.
Haanperä
Marjo
M
Laboratory of Human Microbial Ecology, Department of Bacterial and Inflammatory Diseases, National Public Health Institute, Kiinamyllynkatu 13, FIN-20520 Turku, Finland. marjo.haanpera@ktl.fi
Forssten
Sofia D
SD
Huovinen
Pentti
P
Jalava
Jari
J
D019943:Amino Acid Substitution; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D006801:Humans; D007711:Klebsiella pneumoniae / Q000145:classification / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D017354:Point Mutation; D017422:Sequence Analysis, DNA; D001618:beta-Lactamases / Q000145:classification / Q000235:genetics*
D017931:DNA Primers; D004269:DNA, Bacterial; D001618:beta-Lactamases