Monocyte attachment and migration through collagen IV in diabetes mellitus.
The interactions between monocytes and extracellular matrix proteins have been implicated in atherosclerosis pathophysiology. In the present study we evaluated monocyte attachment and migration through oxidized and non-oxidized collagen IV. Monocyte attachment was tested on microwells coated with either native or oxidized collagen IV. Monocyte migration through collagen IV was examined on transwells. Monocytes derived from patients with diabetes mellitus showed an increased ability to attach and migrate through collagen IV as compared to those derived from healthy volunteers. Moreover, control monocytes attached to oxidized collagen at a higher degree, while they migrated through oxidized collagen at a lower degree, as compared to the native protein. Our results also showed the involvement of the alpha2 integrin subunit in the above phenomena suggesting a modified interaction between monocytes and collagen IV in diabetes mellitus.
Kostidou
Elena
E
Department of Biological Chemistry, Medical School, Aristotle University, Thessaloniki 54124, Greece.
Koliakos
George
G
Paletas
Konstantinos
K
Kaloyianni
Martha
M
D000368:Aged; D000906:Antibodies / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002465:Cell Movement; D024141:Collagen Type IV / Q000737:chemistry*; D003924:Diabetes Mellitus, Type 2 / Q000503:physiopathology*; D006801:Humans; D039421:Integrin alpha2 / Q000276:immunology; D009000:Monocytes / Q000502:physiology*; D010084:Oxidation-Reduction
D000906:Antibodies; D024141:Collagen Type IV; D039421:Integrin alpha2