title: Crystallization and preliminary crystallographic analysis of decameric and monomeric forms of C49S mutant thioredoxin-dependent AhpC from Helicobacter pylori.
abstract: Cys49Ser mutant Helicobacter pylori alkyl hydroperoxide reductase (C49S HpAhpC) was purified under reducing conditions in monomeric and decameric forms. The monomeric form was crystallized by the hanging-drop vapour-diffusion method. The crystals diffracted to 2.25 A resolution and belonged to space group C2, with unit-cell parameters a = 245.8, b = 140.7, c = 189.5 A, beta = 127 degrees , and contained 20 molecules in the asymmetric unit. A crystal of the decameric form was obtained by the microbatch crystallization method and diffracted to 2.8 A resolution. It belonged to space group C222, with unit-cell parameters a = 257.5, b = 417.5, c = 95.6 A. The structure of the monomeric form of C49S HpAhpC has been solved by the molecular-replacement method.
authors.affiliation: Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju 660-701, Republic of Korea.
authors.forename: Kyung Hye
authors.initials: KH
authors.lastname: Seo
authors.forename: Ahmad
authors.initials: A
authors.lastname: Furqoni
authors.forename: Young Chul
authors.initials: YC
authors.lastname: Kwon
authors.forename: Myung Je
authors.initials: MJ
authors.lastname: Cho
authors.forename: Kwang Ho
authors.initials: KH
authors.lastname: Rhee
authors.forename: Sang Yeol
authors.initials: SY
authors.lastname: Lee
authors.forename: Kon Ho
authors.initials: KH
authors.lastname: Lee
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D018360:Crystallography, X-Ray; D016480:Helicobacter pylori / Q000201:enzymology*; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010544:Peroxidases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D017354:Point Mutation; D020836:Protein Structure, Quaternary; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D010544:Peroxidases; C508531:ahpC protein, Helicobacter pylori