DNA that is dispersed in the liquid crystalline phases of phospholipids is actively transcribed.
We report that a 4.3 kbp linearised T7 DNA plasmid is actively transcribed when it is dispersed in the hexagonal liquid crystalline phase of dioleoylphosphoethanolamine (DOPE).
Corsi
Josephine
J
School of Chemistry, University of Southampton, Southampton, UK SO17 1BJ.
Dymond
Marcus K
MK
Ces
Oscar
O
Muck
Joscha
J
Zink
Daniele
D
Attard
George S
GS
D017123:Bacteriophage T7 / Q000201:enzymology / Q000235:genetics; D003460:Crystallization; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics; D005229:Fatty Acids, Monounsaturated / Q000737:chemistry; D008156:Luciferases / Q000235:genetics; D010714:Phosphatidylethanolamines / Q000737:chemistry*; D010957:Plasmids / Q000737:chemistry* / Q000235:genetics; D000644:Quaternary Ammonium Compounds / Q000737:chemistry; D014158:Transcription, Genetic
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D004279:DNA, Viral; D005229:Fatty Acids, Monounsaturated; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D008156:Luciferases; C070046:1,2-dioleoyloxy-3-(trimethylammonium)propane