RNAi knockdown of transcription factor Pu.1 in the differentiation of mouse embryonic stem cells.
Murine embryonic stem (mES) cells are pluripotent cells derived from the inner cell mass of the preimplantation blastocyst. These cells are primitive and undifferentiated and have the potential to become a wide variety of specialized cell types. Mouse ES cells can be regarded as a versatile biological tool that has led to major advances in our understanding of cell and developmental biology. To study specific gene function in early developmental events, gene knockout approaches have been traditionally used, however, this is a time-consuming and expensive approach. Recently, we have shown that small interfering RNA is an effective strategy to knockdown target gene expression, during ES cell differentiation, and consequently, one can alter cell fates in ES-derived differentiated cells. This method will be useful to test the function of a wide variety of gene products using the ES cell differentiation system.
Zou
Gang-Ming
GM
Herman B Wells Center for Pediatric Research, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, USA.
Thompson
Meredith A
MA
Yoder
Mervin C
MC
D000818:Animals; D015153:Blotting, Western / Q000379:methods; D002454:Cell Differentiation; D002478:Cells, Cultured; D053595:Embryonic Stem Cells / Q000378:metabolism*; D007124:Immunoenzyme Techniques / Q000379:methods*; D051379:Mice; D011518:Proto-Oncogene Proteins / Q000037:antagonists & inhibitors*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000494:pharmacology*; D015534:Trans-Activators / Q000037:antagonists & inhibitors*; D000099232:Proto-Oncogene Protein Spi-1
D011518:Proto-Oncogene Proteins; D034741:RNA, Small Interfering; D015534:Trans-Activators; D000099232:Proto-Oncogene Protein Spi-1