cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.
M-CSF is a key cytokine in macrophage development by inducing MAPKs activation, and cAMP can inhibit MAPKs activation induced by inflammatory stimuli. To explore the effects of cAMP on M-CSF-induced MAPKs activation and on macrophage development, the model of bone marrow-derived murine macrophages (BMMs) was used. The effects of cAMP on M-CSF-induced MAPKs activation were analyzed by Western blotting assay, and the effects of cAMP on CD14 and F4/80 expression during macrophage development were examined by FACS analysis. Macrophage morphology showed the successful establishment of the model of macrophage development. Western blotting assay revealed that M-CSF activated ERK, JNK and p38 in both mature and immature macrophages, and cAMP inhibited M-CSF-induced ERK, JNK and p38 activation in a time-dependent manner. FACS analysis revealed that macrophage development was impaired with cAMP pretreatment. In conclusion, cAMP modulates macrophage development by suppressing M-CSF-induced MAPKs activation.
Zhu
Ning
N
Department of Molecular Immunology, Institute of Basic Medical Sciences, Beijing, China.
Cui
Jian
J
Qiao
Chunxia
C
Li
Yan
Y
Ma
Yuanfang
Y
Zhang
Jiyan
J
Shen
Beifen
B
D000818:Animals; D000943:Antigens, Differentiation / Q000235:genetics / Q000378:metabolism; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D002478:Cells, Cultured; D000242:Cyclic AMP / Q000378:metabolism / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D018950:Lipopolysaccharide Receptors / Q000235:genetics / Q000378:metabolism; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects* / Q000201:enzymology / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D013997:Time Factors
D000943:Antigens, Differentiation; D018950:Lipopolysaccharide Receptors; C036763:monocyte-macrophage differentiation antigen; D016173:Macrophage Colony-Stimulating Factor; D000242:Cyclic AMP; D020928:Mitogen-Activated Protein Kinases