title: The adenovirus E1B-55K oncoprotein induces SUMO modification of p53.
abstract: The ability of adenovirus to induce cell transformation depends on the E1A and E1B-55K oncoproteins. While E1A functionally inactivates the retinoblastoma tumour suppressor, E1B-55K primarily interferes with the function of p53. In adenovirus transformed cells E1B-55K can directly affect p53-dependent transactivation. In virus-infected cells E1B-55K additionally cooperates with the viral E4orf6 protein to induce ubiquitin-dependent degradation of p53. Here we unravel a novel activity of E1B-55K by demonstrating that it drastically stimulates the post-translational modification of p53 by the ubiquitin-like SUMO modifier. Consistent with this finding the extent of p53 SUMOylation is highly elevated in adenovirus transformed cell lines. E1B-55K-mediated SUMOylation depends on the direct interaction of E1B-55K with p53 and additionally requires SUMO modification of E1B-55K. These data suggest that E1B-55K exploits both ubiquitin and ubiquitin-like systems to target host cell proteins and thus shed new light on the molecular mechanisms of E1B- 55K function. Moreover, the data expand the emerging concept of dual-specificity factors that act in both the SUMO and ubiquitin pathway and identify E1B-55K as the first viral protein that shares this dual activity.
authors.affiliation: Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Martinsried, Germany. stmuelle@biochem.mpg.de
authors.forename: Stefan
authors.initials: S
authors.lastname: Muller
authors.forename: Thomas
authors.initials: T
authors.lastname: Dobner
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism*; D017501:Adenovirus E1B Proteins / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000235:genetics*; D006801:Humans; D047468:Immunoprecipitation; D008856:Microscopy, Fluorescence; D011499:Protein Processing, Post-Translational / Q000502:physiology*; D025842:SUMO-1 Protein / Q000378:metabolism*; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism*
chemical_list: D017501:Adenovirus E1B Proteins; D025842:SUMO-1 Protein; D016159:Tumor Suppressor Protein p53