Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes.
The aim of this study was to identify active principles of Gentianella austriaca responsible for the reduction of the incidence of micronuclei in irradiated lymphocytes in vitro. The radioprotective effects of ether (EF) and methanolic (MeF) fractions, water-soluble xanthones demethylbellidifolin (1), demethylbellidifolin 8-O-glucoside (2), bellidifolin 8-O-glucoside (3), and flavonoid swertisin (4) against chromosomal damage induced by gamma-rays were determined using the micronucleus test. EF and MeF showed better protection in treatment of human lymphocytes after gamma-irradiation than did isolated compounds. Among the isolated compounds, the effectiveness in reduction of the frequency of micronuclei followed the order 4>3>2>1. The anti-lipoperoxidant activity was in the order 2>4>1, while 3 slightly increased the level of malondialdehyde. In addition, the effectiveness in induction of apoptosis followed the order, 3>2>4, while 1 had no proapoptotic effect. These results suggest that the antioxidative properties of the polyphenols tested may contribute to the radioprotective effects of G. austriaca.
Janković
Teodora
T
Institute for Medicinal Plant Research Josif Pancić, Belgrade, Serbia. tjankovic@iplb.co.yu
Savikin
Katarina
K
Menković
Nebojsa
N
Aljancić
Ivana
I
Leskovac
Andreja
A
Petrović
Sandra
S
Joksić
Gordana
G
D000975:Antioxidants / Q000032:analysis; D017209:Apoptosis / Q000187:drug effects; D001713:Biphenyl Compounds / Q000737:chemistry; D049109:Cell Proliferation / Q000187:drug effects; D005419:Flavonoids / Q000302:isolation & purification* / Q000494:pharmacology; D005720:Gamma Rays; D031313:Gentianella / Q000737:chemistry*; D006801:Humans; D006834:Hydrazines / Q000737:chemistry; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D008315:Malondialdehyde / Q000378:metabolism; D015162:Micronucleus Tests; D010636:Phenols / Q000302:isolation & purification* / Q000494:pharmacology; D010851:Picrates; D059808:Polyphenols; D011837:Radiation-Protective Agents / Q000032:analysis* / Q000494:pharmacology
D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D006834:Hydrazines; D010636:Phenols; D010851:Picrates; D059808:Polyphenols; D011837:Radiation-Protective Agents; D008315:Malondialdehyde; C004931:1,1-diphenyl-2-picrylhydrazyl