Rapid and sensitive detection of norovirus genomes in oysters by a two-step isothermal amplification assay system combining nucleic acid sequence-based amplification and reverse transcription-loop-mediated isothermal amplification assays.
We developed a two-step isothermal amplification assay system, which achieved the detection of norovirus (NoV) genomes in oysters with a sensitivity similar to that of reverse transcription-seminested PCR. The time taken for the amplification of NoV genomes from RNA extracts was shortened to about 3 h.
Fukuda
Shinji
S
Center for Public Health and Environment, Hiroshima Prefectural Technology Research Institute, Minami-machi 1-6-29, Minami-ku, Hiroshima 734-0007, Japan. s-fukuda80723@pref.hiroshima.lg.jp
Sasaki
Yukie
Y
Seno
Masato
M
D000818:Animals; D017931:DNA Primers / Q000235:genetics; D016679:Genome, Viral; D029322:Norovirus / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D010125:Ostreidae / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D048348:Reverse Transcription; D021161:Self-Sustained Sequence Replication; D012680:Sensitivity and Specificity
D017931:DNA Primers; D012367:RNA, Viral