title: Differentiation between canine cutaneous and visceral leishmaniasis by the detection of immunoglobulin G specific for Leishmania (Viannia) braziliensis and Leishmania (Leishmania) chagasi antigens using flow cytometry.
abstract: Flow cytometry employing Leishmania (L.) chagasi (Lc) and L. (Viannia) braziliensis (Lb) antigen was used to establish the differential diagnosis between visceral (VL) and cutaneous leishmaniasis (CL) in dogs. Flow cytometry permitted the detection of Leishmania-specific immunoglobulin G in sera from 19 dogs: nine with CL and 10 with VL. A significant difference in the percentage of positive staining was observed in sera from dogs with CL between the homologous antigen (69% for Lb) and the heterologous antigen (42% for Lc). However, this difference was not significant in sera from dogs with VL (61% for Lb and 73% for Lc). No significant staining was observed in control sera (0.6% for Lb and 0.4% for Lc) consisting of samples from healthy dogs, or in the group with sporotrichosis (1.8% for Lb and 1.5% for Lc), a differential diagnosis of CL. The results suggest that flow cytometry might be useful for the differentiation between CL and VL in dogs, with practical applications in areas where the two infections overlap.
authors.affiliation: Laboratório de Tecnologia Diagnóstica, Instituto de Tecnologia em Imunobiológicos, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.
authors.forename: Marta de Almeida
authors.initials: Mde A
authors.lastname: Santiago
authors.forename: Flávia Coelho
authors.initials: FC
authors.lastname: Ribeiro
authors.forename: Eliame
authors.initials: E
authors.lastname: Mouta-Confort
authors.forename: Lílian Dias
authors.initials: LD
authors.lastname: Nascimento
authors.forename: Armando de Oliveira
authors.initials: Ade O
authors.lastname: Schubach
authors.forename: Maria de Fatima
authors.initials: Mde F
authors.lastname: Madeira
authors.forename: Alvaro Luiz
authors.initials: AL
authors.lastname: Bertho
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D004283:Dog Diseases / Q000175:diagnosis* / Q000276:immunology / Q000469:parasitology; D004285:Dogs; D005434:Flow Cytometry / Q000662:veterinary; D007074:Immunoglobulin G / Q000097:blood*; D007891:Leishmania / Q000145:classification*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D007898:Leishmaniasis, Visceral / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*; D013045:Species Specificity
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G