title: Importance of the Omp25/Omp31 family in the internalization and intracellular replication of virulent B. ovis in murine macrophages and HeLa cells.
abstract: The role of the outer membrane proteins of the Omp25/Omp31 family in invasiveness and intracellular survival of virulent B. ovis in phagocytes was analyzed. The absence of Omp25d or Omp22 in B. ovis abolished its invasive capacity in HeLa cells and reduced it in J774.A1 cells. Additionally, in J774.A1 cells, the Deltaomp25d mutant was unable to multiply, whereas the Deltaomp22 mutant was cleared at 24h post-infection. These findings demonstrate that Omp25d and Omp22 are essential for the invasion and survival of B. ovis inside host cells, and justify the strong attenuation in virulence of the Deltaomp25d and Deltaomp22 mutants.
authors.affiliation: Departamento de Microbiología y Genética, Edificio Departamental, Universidad de Salamanca, Plaza Doctores de la Reina s/n, 37007 Salamanca, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Martín-Martín
authors.forename: Paola
authors.initials: P
authors.lastname: Caro-Hernández
authors.forename: Antonio
authors.initials: A
authors.lastname: Orduña
authors.forename: Nieves
authors.initials: N
authors.lastname: Vizcaíno
authors.forename: Luis
authors.initials: L
authors.lastname: Fernández-Lago
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000502:physiology*; D042061:Brucella ovis / Q000502:physiology*; D002006:Brucellosis / Q000382:microbiology; D006367:HeLa Cells / Q000382:microbiology*; D006801:Humans; D008264:Macrophages / Q000382:microbiology*; D051379:Mice; D014774:Virulence
chemical_list: D001425:Bacterial Outer Membrane Proteins