[Typing of ABO locus with biological microchip as a new level in solution of problems in forensic-medical biological expertise of material evidences].
There are cases in practice when during expertise of material evidences, discrepancies between results of typing of ABO antigens and molecular-genetic typing of DNA occur. In this work, as a radical approach to objective solution of similar conflict situations, for some contradictory case of expertise, all examinations were performed on the unified methodological base--DNA level. Instead of biological (isoserological) typing of ABO antigen, molecular-genetic typing of ABO locus with biological microchip was performed. In all cases the results, received with the use of biological microchip, do not contradict but completely conform to the results of others molecular-genetic examinations performed in the case. Given results indicate irrationality of further use of traditional methods of isoserological typing of ABO antigen for primary differentiation of biological material. These analyses, if necessary, have to be performed on DNA level with molecular-genetic expertise.
Ivanov
P L
PL
Kaganova
N L
NL
Zemskova
E Iu
EIu
Bin'ko
I A
IA
Mitiaeva
O N
ON
Fesenko
D O
DO
Nasedkina
T V
TV
D000017:ABO Blood-Group System / Q000235:genetics* / Q000276:immunology; D000941:Antigens / Q000032:analysis; D001788:Blood Grouping and Crossmatching / Q000379:methods; D004247:DNA / Q000235:genetics*; D053803:Forensic Genetics / Q000379:methods*; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D012680:Sensitivity and Specificity
D000017:ABO Blood-Group System; D000941:Antigens; D004247:DNA