title: [Differential expression of haemolymph proteome of resistant strain and susceptible strain for BmNPV in Bombyx mori L].
abstract: Three model silkworms, highly resistant strain, highly susceptible strain and their near isogenic line were established by hybridization and backcross. The resistance of silkworm (Bombyx mori L.) to BmNPV was studied at proteomic level using two-dimensional gel electrophoresis and MALDI TOF/TOF MS. Differential expression profiles of proteome in resistant strain, susceptible strain and near isogenic line were obtained, and 180, 190 and 187 of protein spots were shown, respectively. Among them, 80% were concentrated in pI 5-9. Twelve differential protein spots in total were obtained from 3 gels. Using MALDI TOF/TOF MS, five proteins, including aminoacylase, were identified from these spots. The exclusive expression of aminoacylase in highly resistant strain and near isogenic line and its absence in susceptible strain suggest that it might be a BmNPV-resistance related protein.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Keya
authors.initials: K
authors.lastname: Cai
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Liu
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D000581:Amidohydrolases / Q000032:analysis* / Q000235:genetics; D000818:Animals; D012831:Bombyx / Q000737:chemistry* / Q000235:genetics / Q000821:virology; D015180:Electrophoresis, Gel, Two-Dimensional; D006458:Hemolymph / Q000737:chemistry*; D054884:Host-Pathogen Interactions / Q000235:genetics*; D019476:Insect Proteins / Q000032:analysis; D017924:Nucleopolyhedroviruses / Q000472:pathogenicity / Q000502:physiology*; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods; D011995:Recombination, Genetic; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D019476:Insect Proteins; D020543:Proteome; D000581:Amidohydrolases; C019672:aminoacylase I