title: Globular adiponectin inhibits GnRH secretion from GT1-7 hypothalamic GnRH neurons by induction of hyperpolarization of membrane potential.
abstract: Reproduction is accurately regulated by metabolic states in mammals. Adiponectin regulates luteinizing hormone (LH) secretion in the pituitary and energy homeostasis in the hypothalamus. We further investigated the gonadotropin-releasing hormone (GnRH) secretion regulation by adiponectin and its related molecular and electrophysiological mechanisms. The results showed that adiponectin receptors (AdipR1 and 2) were expressed in GT1-7 cells derived from hypothalamus neurons. GnRH secretion was inhibited via activation of AMP-activated protein kinase (AMPK). Moreover, we revealed that hyperpolarization of plasma membrane potentials and reduction of calcium influx was also caused by adiponectin.
authors.affiliation: Shanghai Clinical Center for Endocrine and Metabolic Diseases, Shanghai Institute of Endocrinology and Metabolism, Shanghai Key Laboratory of Endocrine Tumors, Endocrine and Metabolic Division, Shanghai Jiao Tong University School of Medicine, China.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Wen
authors.forename: Wen-Shan
authors.initials: WS
authors.lastname: Lv
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.forename: Ai-Fang
authors.initials: AF
authors.lastname: Nie
authors.forename: Xiao-Bing
authors.initials: XB
authors.lastname: Cheng
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Ge
authors.forename: Xiao-Ying
authors.initials: XY
authors.lastname: Li
authors.forename: Guang
authors.initials: G
authors.lastname: Ning
mesh_terms: D000091162:AMP-Activated Protein Kinase Kinases; D052242:Adiponectin / Q000494:pharmacology / Q000502:physiology*; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D004789:Enzyme Activation; D007987:Gonadotropin-Releasing Hormone / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007031:Hypothalamus / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D008564:Membrane Potentials / Q000187:drug effects; D051379:Mice; D009474:Neurons / Q000187:drug effects / Q000378:metabolism; D011494:Protein Kinases / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000378:metabolism
chemical_list: D052242:Adiponectin; D054419:Receptors, Adiponectin; C475419:adiponectin receptor 1, mouse; C475421:adiponectin receptor 2, mouse; D007987:Gonadotropin-Releasing Hormone; D011494:Protein Kinases; D000091162:AMP-Activated Protein Kinase Kinases; D002118:Calcium