title: Does strong hypertrophic condition induce fast mitochondrial DNA mutation of rabbit heart?
abstract: Homo- and heteroplasmic mitochondrial DNA (mtDNA) mutations were observed and identified in an isoproterenol-induced rabbit model of cardiac hypertrophy. Genes encoding proteins essential for catalyzing mitochondrial electron transfer and for generating the proton motive force, such as NADH dehydrogenases (ND2, ND3, ND4, and ND6), cytochrome b, and ATPase 8, showed increased susceptibility for mutation. Specifically, five mutations caused amino acid changes and were located in Complex I and Complex V gene clusters. To our knowledge, this is the first demonstration of a relationship between cardiac hypertrophy induced by a strong sympathetic load and rapid mtDNA mutations.
authors.affiliation: Department of Physiology and Biophysics, School of Medicine, Inje University, Busan 614-735, Republic of Korea.
authors.forename: Taeho
authors.initials: T
authors.lastname: Kim
authors.forename: Vu Thi
authors.initials: VT
authors.lastname: Thu
authors.forename: Il-Yong
authors.initials: IY
authors.lastname: Han
authors.forename: Jae Boum
authors.initials: JB
authors.lastname: Youm
authors.forename: Euiyong
authors.initials: E
authors.lastname: Kim
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Kang
authors.forename: Yang Wook
authors.initials: YW
authors.lastname: Kim
authors.forename: Jae Hwa
authors.initials: JH
authors.lastname: Lee
authors.forename: Hyun
authors.initials: H
authors.lastname: Joo
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D006332:Cardiomegaly / Q000139:chemically induced / Q000235:genetics*; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D042967:Electron Transport Complex I / Q000235:genetics; D007545:Isoproterenol / Q000633:toxicity; D008297:Male; D008929:Mitochondria, Heart / Q000201:enzymology / Q000235:genetics*; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D005810:Multigene Family / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D009206:Myocardium / Q000378:metabolism / Q000473:pathology; D009245:NADH Dehydrogenase / Q000145:classification / Q000235:genetics; D017354:Point Mutation; D011817:Rabbits; D013997:Time Factors
chemical_list: D004272:DNA, Mitochondrial; D045303:Cytochromes b; D009245:NADH Dehydrogenase; D025261:Mitochondrial Proton-Translocating ATPases; D042967:Electron Transport Complex I; D007545:Isoproterenol