title: [Expression and implication of osteopontin in renal cortex of diabetic rats].
abstract: OBJECTIVE
  To investigate the expression and implication of osteopontin (OPN) in renal cortex of diabetic rats.
  
  
  METHODS
  Experimental diabetes was reproduced in uninephrectomized rats by giving streptozotocin (STZ). The animals were divided into two groups: control group and diabetic group (each n=24). Immunohistochemistry and Western blotting were used to detect the protein expression of OPN, CD68, and proliferating cell nuclear antigen (PCNA). At the same time the mRNA expression of OPN was detected by in situ hybridization.
  
  
  RESULTS
  Compared with control group, blood glucose, blood urea nitrogen (BUN), 24-hour urine protein quantum and quantification of OPN protein significantly increased while creatinine clearance rate (CCr) was significantly decreased in diabetic rat group 1, 2, 4 and 8 weeks after STZ injection. The protein of PCNA were also higher than that of control group 1 and 2 weeks after STZ injection (all P<0.05), and the increase in CD68 was observed at week 4 and week 8.
  
  
  CONCLUSIONS
  Overexpression of OPN may be responsible for the proliferation of tubular cells at early stage of diabetic nephropathy and for the accumulation of macrophage at later stage.
authors.affiliation: Department of Pathology, Hebei Medical University, Shijiazhuang 050017, Hebei, China.
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Cao
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Jun
authors.initials: J
authors.lastname: Hao
authors.forename: Yun-Zhuo
authors.initials: YZ
authors.lastname: Ren
authors.forename: Hui-Jun
authors.initials: HJ
authors.lastname: Duan
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D003928:Diabetic Nephropathies / Q000378:metabolism*; D004195:Disease Models, Animal; D008297:Male; D053495:Osteopontin / Q000235:genetics / Q000378:metabolism*; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D053495:Osteopontin; D018809:Proliferating Cell Nuclear Antigen; D012333:RNA, Messenger; C539355:CD68 protein, rat; D000097382:CD68 Molecule