The modified Je-Ho-Tang, Korean herbal medicine, inhibits whole-blood aggregation and platelet adhesion to collagen under flow.
We investigated the in vitro effect of modified Je-Ho-Tang (MJHT) hot-water extract on platelet aggregation and activation induced by agonists in human whole blood, and on platelet adhesion to a collagen-coated surface under flow shear stress conditions. MJHT extract potently inhibited the collagen-induced platelet aggregation in human whole blood in a dose-dependent manner, with a half-maximal inhibitory dose (IC(50)) value of 526 microg/mL. We accessed several markers of platelet activation using receptor expression on platelet membranes, including GPIIb/IIIa-like expression (PAC-1) and P-selectin (CD62); we monitored the intracellular calcium mobilization response by flow cytometry in healthy subjects. A significant decrease in PAC-1 (P=0.002), CD62 (P=0.002), and intracellular calcium mobilization (P<0.001) were seen in the presence of MJHT extract. In addition, we have used image analysis to study human platelet adhesion to collagen under physiologic flow conditions in a perfusion chamber. MJHT extract markedly decreased platelet adhesion to the collagen-coated surface. These results show that MJHT has potent anti-platelet activity.
Jeon
Won Kyung
WK
Department of Herbal Resources Research, Korea Institute of Oriental Medicine, South Korea.
Kim
Yeong Eun
YE
Park
Sun Ok
SO
Kwon
Dae Young
DY
Ahn
Sang Woo
SW
Lee
Jeong Hwa
JH
Ji
Myoung Soon
MS
Ko
Byoung Seob
BS
D000328:Adult; D002118:Calcium / Q000378:metabolism; D003094:Collagen / Q000494:pharmacology*; D005260:Female; D006801:Humans; D055670:Medicine, Korean Traditional; D010936:Plant Extracts / Q000494:pharmacology*; D010973:Platelet Adhesiveness / Q000187:drug effects*; D010974:Platelet Aggregation / Q000187:drug effects*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology*
D010936:Plant Extracts; D010975:Platelet Aggregation Inhibitors; D003094:Collagen; D002118:Calcium