Imaging mitochondrial redox environment and oxidative stress using a redox-sensitive fluorescent protein.
Redox-sensitive green fluorescent protein (roGFP) is a fluorescent protein in which two cysteines are placed adjacently in the barrel structure. Disulfide formation (oxidation) increases the absorption at short wavelengths (410 nm) at the expense of absorption at longer wavelengths (490 nm). The fluorescence ratio indicates reduction/oxidation, i.e., the redox potential at specific cellular locations.
Wolf
Alexander M
AM
Department of Biochemistry and Cell Biology, Institute of Development and Aging Sciences, Graduate School of Medicine, Nippon Medical School, Japan.
Asoh
Sadamitsu
S
Ohsawa
Ikuroh
I
Ohta
Shigeo
S
D049452:Green Fluorescent Proteins; D008928:Mitochondria / Q000502:physiology / Q000648:ultrastructure*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology*
D049452:Green Fluorescent Proteins