title: Characterization of de novo diffuse large B-cell lymphoma with a translocation of c-myc and immunoglobulin genes.
abstract: The characteristics of de novo diffuse large B-cell lymphoma (DLBCL) with translocation of c-myc and immunoglobulin (Ig) genes (c-myc/Ig DLBCL), were investigated in 13 cases of c-myc/Ig DLBCL. Immunohistochemistry revealed five cases were positive for CD10 and BCL6 expression (CD10(+)/BCL6(+)), five cases of CD10(-)/BCL6(+)/MUM1(-), one case of CD10(-)/BCL6(+)/MUM1(+) and two cases of CD10(-)/BCL6(-)/MUM1(+) expression, indicating 10 cases of germinal center B-cell DLBCL and three cases of non-germinal center B-cell DLBCL. Ongoing mutation of the Ig heavy chain gene variable region (IgH-V) was found in two cases with CD10 and BCL6 expression and one case showing CD10(-)/BCL6(+)/MUM1(-) expression. These three cases of ongoing mutation of the IgH-V gene did not express BCL2, unlike those without ongoing mutation. These results suggest a heterogeneous immunophenotype and genotype for c-myc/Ig DLBCL, with CD10(-)/BCL6(+)/MUM1(-) cases the most frequent.
authors.affiliation: Department of Hematology, Tokai University School of Medicine, Isehara, Japan.
authors.forename: Ako
authors.initials: A
authors.lastname: Kikuchi
authors.forename: Naoya
authors.initials: N
authors.lastname: Nakamura
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Kuze
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Masafumi
authors.initials: M
authors.lastname: Abe
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Ohno
authors.forename: Takashi
authors.initials: T
authors.lastname: Akasaka
authors.forename: Shigeo
authors.initials: S
authors.lastname: Nakamura
authors.forename: Koichi
authors.initials: K
authors.lastname: Ohshima
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ando
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D004268:DNA-Binding Proteins / Q000378:metabolism; D005260:Female; D005803:Genes, Immunoglobulin; D016259:Genes, myc; D005838:Genotype; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007135:Immunoglobulin Variable Region / Q000235:genetics*; D007150:Immunohistochemistry; D016130:Immunophenotyping; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009154:Mutation; D015260:Neprilysin / Q000378:metabolism; D051560:Proto-Oncogene Proteins c-bcl-6; D014178:Translocation, Genetic
chemical_list: C084103:BCL6 protein, human; D004268:DNA-Binding Proteins; D007143:Immunoglobulin Heavy Chains; D007135:Immunoglobulin Variable Region; D051560:Proto-Oncogene Proteins c-bcl-6; D015260:Neprilysin