title: Production and recovery of polyunsaturated fatty acids-added lipids from fermented canola.
abstract: Canola flake was investigated as a potential substrate for fungal conversion to produce polyunsaturated fatty acids (PUFAs)-added oil in a 7l fermenter. The results showed that yields of total oil were reduced 9-22% compared to initial oil in the canola flake, but as high as 445mg/l arachidonic acid (ARA, C20:4n6) and 67mg/l eicosapentaenoic acid (EPA, C20:5n3) were produced. The percentages of ARA and EPA of total fatty acids in this fermented oil were 15.5% and 2.3%, respectively. Supercritical CO2 extraction was then investigated for the lipid recovery from fermented canola flake, and extraction kinetics were modeled. The feasibility was demonstrated for production of PUFAs in a laboratory-scale fermentor using canola flake as a single nutrient, and for lipid extraction using supercritical CO2.
authors.affiliation: Biosystems Engineering, Clemson University, Biosystems Research Complex, 51 New Cherry Street, Clemson, SC 29634, USA.
authors.forename: Meidui
authors.initials: M
authors.lastname: Dong
authors.forename: Terry H
authors.initials: TH
authors.lastname: Walker
mesh_terms: D016718:Arachidonic Acid / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism; D015118:Eicosapentaenoic Acid / Q000378:metabolism; D005229:Fatty Acids, Monounsaturated / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000096:biosynthesis*; D005285:Fermentation; D007700:Kinetics; D007753:Laboratories; D008956:Models, Chemical; D010938:Plant Oils; D000074262:Rapeseed Oil
chemical_list: D005229:Fatty Acids, Monounsaturated; D005231:Fatty Acids, Unsaturated; D010938:Plant Oils; D000074262:Rapeseed Oil; D002245:Carbon Dioxide; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid