Potential protective effect of fresh grown unicellular green algae component (resilient factor) against PMA- and UVB-induced MMP1 expression in skin fibroblasts.
Solar UV radiation damages human skin, affecting skin tone and resiliency, and leading to premature ageing (photoageing). Skin damage by oxidants may lead to activation of PKC, thus increasing matrix metalloproteinase (MMPs) expression and collagen degradation. Administration of Chlorella has been shown to play some biochemical functions as well as in vitro inhibition of MMP1 activity. MMP1 secretion was evaluated following PMA treatment or UVB irradiation in the presence of Resilient Factor (RF, aqueous extract fraction of Chlorella), vitamin C, or vitamin E in human skin fibroblasts. Expression levels of MMP1 and elastin protein and of MMP1, TIMP1, and pro-collagen mRNA were also investigated. PMA-induced MMP1 production, protein, and gene expression were suppressed in the presence of RF. Elastin protein diminished after UVB exposure and RF treatment appeared able to counteract the effect of UVB irradiation. Our results also suggest that RF may increase pro-collagen mRNA expression following UVB exposure. This study shows that application of RF prevents MMP1 production via the inhibition of protein and gene expression. In addition, RF prevents the UVB-suppressed elastin protein and pro-collagen gene expression. These findings indicate that RF may exert a protective effect against UVB irradiation-induced damage in the skin.
Shih
Mei-Fen
MF
Department of Pharmacy, Chia-Nan University of Pharmacy and Science, 60 Erh-Jen Road, Sec.1, Pao-An, Jen-Te Hsiang, Tainan, 717, Taiwan ROC. meifenshih@mail.chna.edu.tw
Cherng
Jong-Yuh
JY
D001205:Ascorbic Acid / Q000494:pharmacology; D001685:Biological Factors / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D002708:Chlorella / Q000737:chemistry*; D004151:Dipeptides / Q000494:pharmacology; D004549:Elastin / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000235:genetics / Q000378:metabolism*; D011347:Procollagen / Q000235:genetics / Q000378:metabolism; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015595:Skin Aging / Q000502:physiology / Q000528:radiation effects; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects; D014810:Vitamin E / Q000494:pharmacology
D001685:Biological Factors; D004151:Dipeptides; D004791:Enzyme Inhibitors; C078131:N-(2(R)-2-(hydroxamidocarbonylmethyl)-4-methylpentanoyl)-L-tryptophan methylamide; D011347:Procollagen; D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D014810:Vitamin E; D004549:Elastin; D011493:Protein Kinase C; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1; D013755:Tetradecanoylphorbol Acetate; D001205:Ascorbic Acid