title: Effects of asian sand dust, Arizona sand dust, amorphous silica and aluminum oxide on allergic inflammation in the murine lung.
abstract: The aggravating effects of Asian sand dust (SD) and related minerals on the allergic inflammation were examined in the murine lungs. The toxic materials adsorbed onto Asian SD, Arizona SD were inactivated by heat-treatment. ICR mice were administered mineral samples (0.1 mg/mouse) and/or ovalbumin (OVA) (1 microg/mouse) - normal saline (control), Asian SD, Arizona SD, SiO2, Al2O3, OVA, OVA + Asian SD, OVA + Arizona SD, OVA + SiO2, and OVA + Al2O3 - intratracheally four times at two-week intervals. All samples tested enhanced eosinophil recruitment induced by ovalbumin in the submucosa of the airway, which has a goblet cell proliferation in the bronchial epithelium. Arizona SD alone caused a slight increase of neutrophils in bronchoalveolar lavage fluids along with pro-inflammatory mediators, such as keratinocyte chemoattractant, but Asian SD alone or Al2O3 alone showed no effect. The test particles, except Al2O3, synergistically increased the numbers of eosinophils in BALF induced by ovalbumin. In particular, Arizona SD and SiO2 synergistically increased the eosinophil relevant cytokine and chemokine, such as IL-5 and monocyte chemotactic protein (MCP)-3. The aggravating effects of the samples were dependent on the SiO2 content. All samples tested also induced the adjuvant effects to specific IgG1 production by OVA. These results suggest that the aggravated allergic inflammation by mineral dusts may be due to the mineral elements (mainly SiO2). The enhancement by Arizona SD may be mediated, at least partially, by the increased expression of IL-5 and MCP-3 and also by the modulated expression of IL-5 and MCP-3.
authors.affiliation: Department of Health Sciences, Oita University of Nursing and Health Sciences, Oita, Japan.
authors.forename: Takamich
authors.initials: T
authors.lastname: Ichinose
authors.forename: Seiichi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Kaori
authors.initials: K
authors.lastname: Sadakane
authors.forename: Hrohisa
authors.initials: H
authors.lastname: Takano
authors.forename: Rie
authors.initials: R
authors.lastname: Yanagisawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Inoue
authors.forename: Masataka
authors.initials: M
authors.lastname: Nishikawa
authors.forename: Ikuko
authors.initials: I
authors.lastname: Mori
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Kawazato
authors.forename: Aiko
authors.initials: A
authors.lastname: Yasuda
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shibamoto
mesh_terms: D000537:Aluminum Oxide / Q000633:toxicity*; D000818:Animals; D001130:Arizona; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D002681:China; D016207:Cytokines / Q000276:immunology; D004391:Dust / Q000032:analysis; D004804:Eosinophils / Q000276:immunology; D020397:Goblet Cells / Q000276:immunology; D007073:Immunoglobulin E / Q000097:blood / Q000276:immunology; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007249:Inflammation / Q000097:blood / Q000139:chemically induced / Q000276:immunology / Q000473:pathology; D008070:Lipopolysaccharides / Q000032:analysis; D008168:Lung / Q000187:drug effects* / Q000276:immunology / Q000473:pathology; D008214:Lymphocytes / Q000276:immunology; D008297:Male; D008670:Metals / Q000032:analysis; D051379:Mice; D008813:Mice, Inbred ICR; D010047:Ovalbumin / Q000276:immunology; D010316:Particle Size; D012130:Respiratory Hypersensitivity / Q000097:blood / Q000139:chemically induced* / Q000276:immunology / Q000473:pathology; D012822:Silicon Dioxide / Q000032:analysis / Q000633:toxicity*; D047071:beta-Glucans / Q000032:analysis
chemical_list: D016207:Cytokines; D004391:Dust; D007074:Immunoglobulin G; D008070:Lipopolysaccharides; D008670:Metals; D047071:beta-Glucans; D007073:Immunoglobulin E; D012822:Silicon Dioxide; D010047:Ovalbumin; D000537:Aluminum Oxide