Modeling an active conformation for linear peptides and design of a competitive inhibitor for HMG-CoA reductase.
This study presents an approach that can be used to search for lead peptide candidates, including unconstrained structures in a recognized sequence. This approach was performed using the design of a competitive inhibitor for 3-hydroxy-3-methylglutaryl CoA reductase (HMGR). In a previous design for constrained peptides, a head-to-tail cyclic structure of peptide was used as a model of linear analog in searches for lead peptides with a structure close to an active conformation. Analysis of the conformational space occupied by the peptides suggests that an analogical approach can be applied for finding a lead peptide with an unconstrained structure in a recognized sequence via modeling a cycle using fixed residues of the peptide backbone. Using the space obtained by an analysis of the bioactive conformations of statins, eight cyclic peptides were selected for a peptide library based on the YVAE sequence as a recognized motif. For each cycle, the four models were assessed according to the design criterion ("V" parameter) applied for constrained peptides. Three cyclic peptides (FGYVAE, FPYVAE, and FFYVAE) were selected as lead cycles from the library. The linear FGYVAE peptide (IC(50) = 0.4 microM) showed a 1200-fold increase the inhibitory activity compared to the first isolated LPYP peptide (IC(50) = 484 microM) from soybean. Experimental analysis of the modeled peptide structures confirms the appropriateness of the proposed approach for the modeling of active conformations of peptides.
Pak
Valeriy V
VV
Food Fusion and Complex Research Division, Korea Food Research Institute, Baekhyun-Dong, Bundang-Ku, Songnam-Si, Kyongki-Do 463-746, Republic of Korea.
Koo
Minseon
M
Kim
Min Jung
MJ
Yang
Hye Jeong
HJ
Yun
Lyubov
L
Kwon
Dae Young
DY
D002942:Circular Dichroism; D015195:Drug Design; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000737:chemistry*; D008958:Models, Molecular; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D011487:Protein Conformation
D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D019151:Peptide Library; D010455:Peptides