Optical methods for the measurement and manipulation of cytosolic free calcium in neutrophils.
The measurement and manipulation of cytosolic free Ca2+ permits the investigation of the mechanisms of generation of the Ca2+ signal and cellular responses to these Ca2+ signals within living neutrophils. The optical methods most applicable to neutrophils, which will be discussed here, are (1) the use of fluorescent indicators of Ca2+ and (2) photoactivation of reagents involved in Ca2+ signaling. Both of these synthetic agents can be loaded into neutrophils as lipid-soluble esters or can be microinjected into the cell. In this chapter, we will outline some of the techniques that have been used to monitor, visualize, and manipulate Ca2+ in neutrophils.
Hillson
Esther J
EJ
Neutrophil Signalling Group, School of Medicine, Cardiff University, Heath Park, United Kingdom.
Dewitt
Sharon
S
Hallett
Maurice B
MB
D000818:Animals; D002118:Calcium / Q000032:analysis* / Q000378:metabolism; D020013:Calcium Signaling; D003600:Cytosol / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000493:pharmacokinetics; D005470:Fluorometry / Q000379:methods; D006801:Humans; D009504:Neutrophils / Q000737:chemistry* / Q000378:metabolism; D055095:Optics and Photonics
D005456:Fluorescent Dyes; D002118:Calcium