title: Crevicular fluid endothelin-1 levels in periodontal health and disease.
abstract: OBJECTIVE
  Endothelin-1 is a 21-amino-acid peptide with multifunctional regulation. Initial research indicated that endothelin-1 levels in the gingival crevicular fluid from patients with chronic periodontitis were higher than those in the gingival crevicular fluid from healthy subjects. The aim of the present study was to assess the relationship between the clinical parameters and the concentrations of endothelin-1 within the gingival crevicular fluid from inflamed gingiva and periodontitis sites and, subsequently, after the treatment of periodontitis sites.
  
  
  METHODS
  A total of 60 subjects were divided into three groups - healthy (group I), gingivitis (group II) and chronic periodontitis (group III) - based on gingival index, pocket probing depth and clinical attachment loss. A fourth group consisted of 20 subjects from group III, 6-8 wk after treatment (i.e. scaling and root planing). Gingival crevicular fluid samples collected from each patient were quantified for endothelin-1 using an enzymatic immunometric assay.
  
  
  RESULTS
  Endothelin-1 was not detected in any sample from any of the study groups.
  
  
  CONCLUSIONS
  The results showed that all the gingival crevicular fluid samples were negative for the endothelin-1 molecule. Therefore, endothelin-1 cannot be considered as a potential biomarker of periodontal disease progression.
authors.affiliation: Department of Periodontics, Government Dental College and Hospital, Bangalore, Karnataka, India. periodontics_gdc@yahoo.co.in
authors.forename: A R
authors.initials: AR
authors.lastname: Pradeep
authors.forename: C N
authors.initials: CN
authors.lastname: Guruprasad
authors.forename: P
authors.initials: P
authors.lastname: Swati
authors.forename: C
authors.initials: C
authors.lastname: Shikha
mesh_terms: D000328:Adult; D015415:Biomarkers; D016022:Case-Control Studies; D002908:Chronic Disease; D019332:Endothelin-1 / Q000032:analysis / Q000378:metabolism*; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry; D005891:Gingivitis / Q000378:metabolism*; D006801:Humans; D008297:Male; D010518:Periodontitis / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D019332:Endothelin-1