title: Microbulbifer agarilyticus sp. nov. and Microbulbifer thermotolerans sp. nov., agar-degrading bacteria isolated from deep-sea sediment.
abstract: Nine agar-degrading strains, designated JAMB A3T, JAMB A7, JAMB A24, JAMB A33, JAMB A94T, JAMM 0654, JAMM 0793, JAMM 1327 and JAMM 1340, were isolated from deep-sea sediment in Suruga Bay and Sagami Bay and off Kagoshima, Japan. On the basis of 16S rRNA gene sequence analysis, the strains were found to be closely affiliated with members of the genera Microbulbifer and Thalassomonas. The hybridization values for DNA-DNA relatedness between two of these strains and Microbulbifer reference strains were significantly lower than that accepted as the phylogenetic definition of a species. On the basis of their distinct taxonomic characteristics, six of the isolated strains represent two novel species of the genus Microbulbifer, for which the names Microbulbifer agarilyticus sp. nov. (type strain JAMB A3T =JCM 14708T =DSM 19200T) and Microbulbifer thermotolerans sp. nov. (type strain JAMB A94T =JCM 14709T =DSM 19189T) are proposed.
authors.affiliation: Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokosuka 237-0061, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Miyazaki
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Nogi
authors.forename: Yukari
authors.initials: Y
authors.lastname: Ohta
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hatada
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Fujiwara
authors.forename: Susumu
authors.initials: S
authors.lastname: Ito
authors.forename: Koki
authors.initials: K
authors.lastname: Horikoshi
mesh_terms: D000362:Agar / Q000378:metabolism; D034241:Alteromonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D000362:Agar