title: Detrimental effects of prenatal exposure to filtered diesel exhaust on mouse spermatogenesis.
abstract: We recently showed that prenatal exposure to diesel exhaust (DE) disrupts spermatogenesis in mouse offspring. This study was undertaken to determine whether filtered DE in which 99.97% of diesel exhaust particles >0.3 microm in diameter were removed affects spermatogenesis in growing mice. After prenatal exposure to filtered DE for 2-16 days postcoitum, we examined daily sperm production (DSP), testicular histology, serum testosterone levels and mRNA expression of hormone synthesis process-related factors. In the filtered DE exposed group, DSP was markedly reduced at 12 weeks compared with the control group; clean air exposed group. Histological examination showed multinucleated giant cells and partial vacuolation in the seminiferous tubules of the exposed group. Testosterone was elevated significantly at 5 weeks. Moreover, luteinizing hormone receptor mRNA at 5 and 12 weeks, 17alpha-hydroxylase/C17-20-lyase and 17beta-hydroxysteroid dehydrogenase mRNAs at 12 weeks were significantly elevated. These results suggest that filtered DE retains its toxic effects on the male reproductive system following prenatal exposure.
authors.affiliation: Department of Hygiene Chemistry, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan.
authors.forename: Naoka
authors.initials: N
authors.lastname: Ono
authors.forename: Shigeru
authors.initials: S
authors.lastname: Oshio
authors.forename: Yuichiro
authors.initials: Y
authors.lastname: Niwata
authors.forename: Seiichi
authors.initials: S
authors.lastname: Yoshida
authors.forename: Naomi
authors.initials: N
authors.lastname: Tsukue
authors.forename: Isamu
authors.initials: I
authors.lastname: Sugawara
authors.forename: Hirohisa
authors.initials: H
authors.lastname: Takano
authors.forename: Ken
authors.initials: K
authors.lastname: Takeda
mesh_terms: D015067:17-Hydroxysteroid Dehydrogenases / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D015726:Giant Cells / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010316:Particle Size; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D012333:RNA, Messenger / Q000187:drug effects / Q000378:metabolism; D011974:Receptors, LH / Q000187:drug effects / Q000378:metabolism; D012671:Seminiferous Tubules / Q000187:drug effects; D013076:Sperm Count; D013091:Spermatogenesis / Q000187:drug effects*; D013254:Steroid 17-alpha-Hydroxylase / Q000187:drug effects / Q000378:metabolism; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D013739:Testosterone / Q000097:blood; D013997:Time Factors; D014617:Vacuoles / Q000187:drug effects / Q000378:metabolism; D001335:Vehicle Emissions / Q000633:toxicity*
chemical_list: D012333:RNA, Messenger; D011974:Receptors, LH; D001335:Vehicle Emissions; D013739:Testosterone; D015067:17-Hydroxysteroid Dehydrogenases; D013254:Steroid 17-alpha-Hydroxylase