C-type natriuretic peptide regulation of limb mesenchymal chondrogenesis is accompanied by altered N-cadherin and collagen type X-related functions.
AMDM, a form of osteochondrodysplasia, is due to the loss-of-function mutations in NPR-B gene. This study investigated the functional involvement of CNP-3, chick homolog of human CNP, and its receptor NPR-B in chondrogenesis utilizing the micromass culture of the chick limb mesenchymal cells. Results revealed CNP-3 and NPR-B expression in the chick limb bud making stage-specific peak levels first at Hamburger-Hamilton stage 23-24, and second at stage 30-31, corresponding to pre-chondrogenic mesenchymal condensation and initiation of chondrogenic maturation-hypertrophy in vivo, respectively. CNP-3 and NPR-B expression in vitro increased parallel to collagen type X expression, but not to that of collagen type II. Treatment of cultures with CNP significantly increased N-cadherin, and collagen type X expression, glycosaminoglycan synthesis and chondrogenesis. Collagen type II expression was not significantly affected. Thus, results implicated CNP-3/NPR-B signaling in pre-chondrogenic mesenchymal condensation, glycosaminoglycan synthesis and late differentiation of chondrocytes in the process of endochondral ossification.
Alan
Tulay
T
Department of Histology and Embryology, Institute of Health Sciences, Pamukkale University, Denizli, Turkey.
Tufan
A Cevik
AC
D000818:Animals; D015415:Biomarkers; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002642:Chick Embryo; D020219:Chondrogenesis; D024121:Collagen Type X / Q000378:metabolism*; D005121:Extremities / Q000196:embryology*; D005786:Gene Expression Regulation; D066298:In Vitro Techniques; D008648:Mesoderm / Q000378:metabolism*; D020098:Natriuretic Peptide, C-Type / Q000235:genetics / Q000378:metabolism*
D015415:Biomarkers; D015820:Cadherins; D024121:Collagen Type X; D020098:Natriuretic Peptide, C-Type