Nuclear magnetic resonance-based modeling and refinement of protein three-dimensional structures and their complexes.
Nuclear magnetic resonance (NMR) has become a well-established method to characterize the structures of biomolecules in solution. High-quality structures are now produced, thanks to both experimental and computational developments, allowing the use of new NMR parameters and improved protocols and force fields in structure calculation and refinement. In this chapter, we give a short overview of the various types of NMR data that can provide structural information, and then focus on the structure calculation methodology itself. We discuss and illustrate with tutorial examples both "classical" structure calculation and refinement approaches as well as more recently developed protocols for modeling biomolecular complexes.
Fuentes
Gloria
G
Bijvoet Center for Biomolecular Research, Utrecht University, Utrecht, The Netherlands.
van Dijk
Aalt D J
AD
Bonvin
Alexandre M J J
AM
D006860:Hydrogen Bonding; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008958:Models, Molecular; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000737:chemistry; D012984:Software; D012986:Software Validation
D011506:Proteins