title: Hazards to hearing from combined exposure to toluene and noise in rats.
abstract: BACKGROUND
  The main risk of hearing impairment from workplace exposure to organic solvents may stem from the potentiation of effects caused by concomitant noise exposure. The aim of the present study was to identify the main hazards from combined long-term, low-level exposure to noise and aromatic organic solvents, like toluene, in rats.
  
  
  METHODS
  The rats were exposed to steady-state, wide-band noise (WBN) and 0 ppm, 100 ppm, 200 ppm and 500 ppm toluene for 90 days. Hearing was assessed using Auditory Brain Stem Responses (ABR) and Distortion Product Oto-Acoustic Emissions (DPOAE) eight weeks after exposure. The impact of noise composition on the interaction between solvent and noise exposure was investigated in rats exposed for 10 days either to 0 ppm, 500 ppm, 1000 ppm or 1500 ppm toluene and either WBN or impulse noise. ABR and DPOAE tests were performed before and two weeks after exposure.
  
  
  RESULTS
  Long-term exposure of rats to WBN and toluene at 500 ppm or less did not show any increase in hearing impairment, compared to the rats exposed to noise only. Synergistic interaction was demonstrated in short-term exposure to 1500 ppm toluene and both to WBN and impulse noise, but hearing impairment was much larger when following exposure to impulse noise.
  
  
  CONCLUSIONS
  In combined exposure to low-levels of noise and toluene, even a long-term exposure did not reveal a potential hazard of hearing impairment. Synergistic interaction in combined short-term exposure to toluene and noise was noted both with respect to WBN and impulse noise, but the impulse noise was much more disruptive than WBN at the same level of noise exposure. The ototoxicity of organic solvents may primarily be a hazard also to human hearing due to the exacerbation of hearing loss by a possible co-exposure to especially harmful noise, such as impulse noise.
authors.affiliation: National Research Centre for the Working Environment, Copenhagen, Denmark. spl@nrcwe.dk
authors.forename: Søren P
authors.initials: SP
authors.lastname: Lund
authors.forename: Gitte B
authors.initials: GB
authors.lastname: Kristiansen
mesh_terms: D000818:Animals; D003718:Denmark; D034381:Hearing Loss / Q000139:chemically induced*; D008297:Male; D009622:Noise / Q000009:adverse effects*; D016273:Occupational Exposure; D051381:Rats; D017208:Rats, Wistar; D014050:Toluene / Q000008:administration & dosage / Q000009:adverse effects*
chemical_list: D014050:Toluene