title: Characterization of Hawaiian isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid.
abstract: We report here the isolation and characterization of three distinct isolates of Cymbidium mosaic virus (CymMV) co-infecting Dendrobium orchid in Hawaii. Isolates 1 and 2 were phylogenetically distinct from previously reported CymMV isolates. However, isolate 3 was highly similar to previously reported CymMV sequences and could be localised to CymMV subgroup A. Isolate 2 localised to CymMV subgroup B. Thus, we report here the first full-length CymMV subgroup B isolate. Isolate 1 represents a recombination event between isolates 2 and 3. Infectivity assays revealed that all three isolates are functional and individually infectious in both Dendrobium and indicator species.
authors.affiliation: Department of Tropical Plant and Soil Sciences, University of Hawaii, 3190 Maile Way, Honolulu, HI 98622, USA. simon.vaughan@bbsrc.ac.uk
authors.forename: S P
authors.initials: SP
authors.lastname: Vaughan
authors.forename: M
authors.initials: M
authors.lastname: Grisoni
authors.forename: M H
authors.initials: MH
authors.lastname: Kumagai
authors.forename: A R
authors.initials: AR
authors.lastname: Kuehnle
mesh_terms: D006254:Hawaii; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000821:virology*; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D017863:Potexvirus / Q000145:classification / Q000235:genetics / Q000472:pathogenicity; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid; D014774:Virulence