title: Differential contribution of cardiac sarcomeric proteins in the myofibrillar force response to stretch.
abstract: The present study examined the contribution of myofilament contractile proteins to regional function in guinea pig myocardium. We investigated the effect of stretch on myofilament contractile proteins, Ca(2+) sensitivity, and cross-bridge cycling kinetics (K (tr)) of force in single skinned cardiomyocytes isolated from the sub-endocardial (ENDO) or sub-epicardial (EPI) layer. As observed in other species, ENDO cells were stiffer, and Ca(2+) sensitivity of force at long sarcomere length was higher compared with EPI cells. Maximal K (tr) was unchanged by stretch, but was higher in EPI cells possibly due to a higher alpha-MHC content. Submaximal Ca(2+)-activated K (tr) increased only in ENDO cells with stretch. Stretch of skinned ENDO muscle strips induced increased phosphorylation in both myosin-binding protein C and myosin light chain 2. We concluded that transmural MHC isoform expression and differential regulatory protein phosphorylation by stretch contributes to regional differences in stretch modulation of activation in guinea pig left ventricle.
authors.affiliation: INSERM, U 637, Université MONTPELLIER I, UFR de Médecine, F-34295, Montpellier, France.
authors.forename: Younss
authors.initials: Y
authors.lastname: Ait Mou
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: le Guennec
authors.forename: Emilio
authors.initials: E
authors.lastname: Mosca
authors.forename: Pieter P
authors.initials: PP
authors.lastname: de Tombe
authors.forename: Olivier
authors.initials: O
authors.lastname: Cazorla
mesh_terms: D000465:Algorithms; D000818:Animals; D002118:Calcium / Q000502:physiology; D024722:Cardiac Myosins / Q000502:physiology; D002352:Carrier Proteins / Q000096:biosynthesis / Q000235:genetics; D002469:Cell Separation; D003285:Contractile Proteins / Q000502:physiology*; D004699:Endocardium / Q000502:physiology; D006168:Guinea Pigs; D006321:Heart / Q000502:physiology*; D008465:Mechanoreceptors / Q000502:physiology*; D009200:Myocardial Contraction / Q000502:physiology; D009206:Myocardium / Q000166:cytology / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000502:physiology; D009210:Myofibrils / Q000502:physiology*; D018994:Myosin Light Chains / Q000502:physiology; D010766:Phosphorylation; D012518:Sarcomeres / Q000502:physiology* / Q000648:ultrastructure; D000099163:Myosin Binding Protein C
chemical_list: D002118:Calcium; D024722:Cardiac Myosins; D002352:Carrier Proteins; D003285:Contractile Proteins; D018994:Myosin Light Chains; D000099163:Myosin Binding Protein C; C107184:myosin light chain 2