title: Effects of a tetramethoxyhydroxyflavone p7f on the expression of inflammatory mediators in LPS-treated human synovial fibroblast and macrophage cells.
abstract: The inhibitory effects of 5,6,3',5'-tetramethoxy 7,4'-hydroxyflavone (labeled as p7F) were elucidated on the productions of proinflammatory cytokines as well as inflammatory mediators in human synovial fibroblasts and macrophage cells. p7F inhibited IL-1beta or TNF-alpha induced expressions of inflammatory mediators (ICAM-1, COX-2, and iNOS). p7F also inhibited LPS-induced productions of nitric oxide and prostaglandin E2 in RAW 264.7 cells. In order to investigate whether p7F would inhibit IL-1 signaling, p7F was added to the D10S Th2 cell line (which is responsive to only IL-1beta and thus proliferates), revealing that p7F inhibited IL-1beta-induced proliferation of D10S Th2 cells in a doseresponse manner. A flow cytometric analysis revealed that p7F reduced the intracellular level of free radical oxygen species in RAW 264.7 cells treated with hydrogen peroxide. p7F inhibited IkappaB degradation and NF-kappaB activation in macrophage cells treated with LPS, supporting that p7F could inhibit signaling mediated via toll-like receptor. Taken together, p7F has inhibitory effects on LPS-induced productions of inflammatory mediators on human synovial fibroblasts and macrophage cells and thus has the potential to be an antiinflammatory agent for inhibiting inflammatory responses.
authors.affiliation: Division of Bioscience and Biotechnology, Konkuk University, Hwayang dong 1, Kwangjin gu, Seoul 143-701, Korea.
authors.forename: Do-Young
authors.initials: DY
authors.lastname: Yoon
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Cho
authors.forename: Jung-Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Eun-Jin
authors.initials: EJ
authors.lastname: Kim
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Kang
authors.forename: Eun-Hee
authors.initials: EH
authors.lastname: Seo
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Shim
authors.forename: Soo-Hyun
authors.initials: SH
authors.lastname: Kim
authors.forename: Hee-Gu
authors.initials: HG
authors.lastname: Lee
authors.forename: Goo-Taeg
authors.initials: GT
authors.lastname: Oh
authors.forename: Jin-Tae
authors.initials: JT
authors.lastname: Hong
authors.forename: Joo-Won
authors.initials: JW
authors.lastname: Park
authors.forename: Jong-Wan
authors.initials: JW
authors.lastname: Kim
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy* / Q000276:immunology / Q000503:physiopathology; D002061:Bursa, Synovial / Q000187:drug effects* / Q000276:immunology / Q000503:physiopathology; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000276:immunology; D005419:Flavonoids / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D007249:Inflammation / Q000188:drug therapy / Q000276:immunology / Q000503:physiopathology; D008070:Lipopolysaccharides / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D010936:Plant Extracts / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000037:antagonists & inhibitors / Q000378:metabolism
chemical_list: C501197:5,6,3',5'-tetramethoxy-7,4'-hydroxyflavone; D000893:Anti-Inflammatory Agents; D016207:Cytokines; D005419:Flavonoids; D008070:Lipopolysaccharides; D010936:Plant Extracts; D017382:Reactive Oxygen Species