title: UPLC-TOF-MS for plant metabolomics: a sequential approach for wound marker analysis in Arabidopsis thaliana.
abstract: The model plant Arabidopsis thaliana was studied for the search of new metabolites involved in wound signalling. Diverse LC approaches were considered in terms of efficiency and analysis time and a 7-min gradient on a UPLC-TOF-MS system with a short column was chosen for metabolite fingerprinting. This screening step was designed to allow the comparison of a high number of samples over a wide range of time points after stress induction in positive and negative ionisation modes. Thanks to data treatment, clear discrimination was obtained, providing lists of potential stress-induced ions. In a second step, the fingerprinting conditions were transferred to longer column, providing a higher peak capacity able to demonstrate the presence of isomers among the highlighted compounds.
authors.affiliation: Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences, EPGL, University of Geneva, Geneva, Switzerland.
authors.forename: Elia
authors.initials: E
authors.lastname: Grata
authors.forename: Julien
authors.initials: J
authors.lastname: Boccard
authors.forename: Davy
authors.initials: D
authors.lastname: Guillarme
authors.forename: Gaetan
authors.initials: G
authors.lastname: Glauser
authors.forename: Pierre-Alain
authors.initials: PA
authors.lastname: Carrupt
authors.forename: Edward E
authors.initials: EE
authors.lastname: Farmer
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Wolfender
authors.forename: Serge
authors.initials: S
authors.lastname: Rudaz
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019295:Computational Biology / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010935:Plant Diseases; D010944:Plants / Q000378:metabolism*; D025341:Principal Component Analysis