title: Large-scale phosphorylation mapping reveals the extent of tyrosine phosphorylation in Arabidopsis.
abstract: Protein phosphorylation regulates a wide range of cellular processes. Here, we report the proteome-wide mapping of in vivo phosphorylation sites in Arabidopsis by using complementary phosphopeptide enrichment techniques coupled with high-accuracy mass spectrometry. Using unfractionated whole cell lysates of Arabidopsis, we identified 2597 phosphopeptides with 2172 high-confidence, unique phosphorylation sites from 1346 proteins. The distribution of phosphoserine, phosphothreonine, and phosphotyrosine sites was 85.0, 10.7, and 4.3%. Although typical tyrosine-specific protein kinases are absent in Arabidopsis, the proportion of phosphotyrosines among the phospho-residues in Arabidopsis is similar to that in humans, where over 90 tyrosine-specific protein kinases have been identified. In addition, the tyrosine phosphoproteome shows features distinct from those of the serine and threonine phosphoproteomes. Taken together, we highlight the extent and contribution of tyrosine phosphorylation in plants.
authors.affiliation: Institute for Advanced Biosciences, Keio University, Tsuruoka, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Sugiyama
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nakagami
authors.forename: Keiichi
authors.initials: K
authors.lastname: Mochida
authors.forename: Arsalan
authors.initials: A
authors.lastname: Daudi
authors.forename: Masaru
authors.initials: M
authors.lastname: Tomita
authors.forename: Ken
authors.initials: K
authors.lastname: Shirasu
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishihama
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry / Q000378:metabolism*; D017124:Conserved Sequence; D010750:Phosphoproteins / Q000737:chemistry; D010766:Phosphorylation; D010768:Phosphoserine / Q000032:analysis; D010769:Phosphothreonine / Q000032:analysis; D019000:Phosphotyrosine / Q000032:analysis* / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry; D017434:Protein Structure, Tertiary; D020543:Proteome / Q000032:analysis / Q000737:chemistry; D017386:Sequence Homology, Amino Acid
chemical_list: D010750:Phosphoproteins; D010940:Plant Proteins; D020543:Proteome; D010769:Phosphothreonine; D010768:Phosphoserine; D019000:Phosphotyrosine