Fibromodulin gene is expressed in human epidermal keratinocytes in culture and in human epidermis in vivo.
Fibromodulin is a small leucine-rich proteoglycan that has a central role in the maintenance of collagen fibrils structure, and in regulation of TGF-beta biological activity. Although, it is mainly found in cartilage and tendon, little is known regarding the expression of the fibromodulin gene in other cell types. By RT-PCR, real time PCR and immunohistochemistry, we describe the expression of the fibromodulin gene and the presence of the protein in human epidermal keratinocytes (HEK), both in culture and in normal human epidermis. Our results show, for the first time, that fibromodulin gene is constantly expressed in HEK during culture time. Immunostaining showed that fibromodulin is located intracytoplasmically in basal and stratified keratinocytes of the growing colonies, confluent cultures, and epidermis in vivo. The expression and intracellular localization of fibromodulin in HEK is a new finding and opens new possible biological roles for the SLRP family.
Vélez-Delvalle
Cristina
C
Department of Cell Biology, Centro de Investigación y de Estudios Avanzados del IPN, Apdo. Postal 14-740, México City 07000, Mexico.
Marsch-Moreno
Meytha
M
Castro-Muñozledo
Federico
F
Bolivar-Flores
Yesid Jaime
YJ
Kuri-Harcuch
Walid
W
D002478:Cells, Cultured; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D000078404:Epidermal Cells; D004817:Epidermis / Q000737:chemistry / Q000378:metabolism*; D016326:Extracellular Matrix Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D000071235:Fibromodulin; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D015603:Keratinocytes / Q000737:chemistry / Q000378:metabolism; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D011509:Proteoglycans / Q000032:analysis / Q000235:genetics* / Q000378:metabolism
D016326:Extracellular Matrix Proteins; C000604330:FMOD protein, human; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins; D011509:Proteoglycans; D000071235:Fibromodulin