title: Survey of Argentinean human plasma for ochratoxin A.
abstract: The presence of ochratoxin A (OTA) in human blood has been reported for many countries, especially in Europe. However, so far no report exists concerning such a presence in Argentina. The aim of this study was to assess OTA concentration in human plasma in two different areas of Buenos Aires province. OTA was determined by high-performance liquid chromatography (HPLC) in 199 plasma samples from blood donors in Mar del Plata and 236 from General Rodriguez. Solid-phase extraction with Bakerbond C-18 cartridge and a final purification with Ochraprep immunoaffinity columns was employed. The limit of quantification of ochratoxin A was 0.019ngml(-1) and the confirmation of OTA was by formation of ochratoxin A methyl ester. The results showed that 63.8% of human plasma samples from Mar del Plata and 62.3% from General Rodriguez were positive for OTA, with Winsorized means of 0.15 and 0.43ngml(-1), respectively. It is important to continue the research to detect the foods responsible of the presence of OTA in plasma.
authors.affiliation: Fundacion de Investigaciones Cientificas Teresa Benedicta de la Cruz, Centro de Investigacion en Micotoxinas, Universidad Nacional de Lujan, Buenos Aires, Argentina. apacin@gmail.com
authors.forename: A M
authors.initials: AM
authors.lastname: Pacin
authors.forename: E V
authors.initials: EV
authors.lastname: Ciancio Bovier
authors.forename: E
authors.initials: E
authors.lastname: Motta
authors.forename: S L
authors.initials: SL
authors.lastname: Resnik
authors.forename: D
authors.initials: D
authors.lastname: Villa
authors.forename: M
authors.initials: M
authors.lastname: Olsen
mesh_terms: D000328:Adult; D000383:Agriculture; D001118:Argentina; D002853:Chromatography, Liquid / Q000379:methods; D005260:Female; D005506:Food Contamination / Q000032:analysis*; D006801:Humans; D008297:Male; D009793:Ochratoxins / Q000097:blood*; D013223:Statistics as Topic
chemical_list: D009793:Ochratoxins; C025589:ochratoxin A