title: Parallel confocal detection of single molecules in real time.
abstract: The confocal detection principle is extended to a highly parallel optical system that continuously analyzes thousands of concurrent sample locations. This is achieved through the use of a holographic laser illumination multiplexer combined with a confocal pinhole array before a prism dispersive element used to provide spectroscopic information from each confocal volume. The system is demonstrated to detect and identify single fluorescent molecules from each of several thousand independent confocal volumes in real time.
authors.affiliation: Pacific Biosciences Inc., 1505 Adams Drive, Menlo Park, California 94025, USA.
authors.forename: Paul M
authors.initials: PM
authors.lastname: Lundquist
authors.forename: Cheng F
authors.initials: CF
authors.lastname: Zhong
authors.forename: Peiqian
authors.initials: P
authors.lastname: Zhao
authors.forename: Austin B
authors.initials: AB
authors.lastname: Tomaney
authors.forename: Paul S
authors.initials: PS
authors.lastname: Peluso
authors.forename: John
authors.initials: J
authors.lastname: Dixon
authors.forename: Brad
authors.initials: B
authors.lastname: Bettman
authors.forename: Yves
authors.initials: Y
authors.lastname: Lacroix
authors.forename: Deborah P
authors.initials: DP
authors.lastname: Kwo
authors.forename: Etienne
authors.initials: E
authors.lastname: McCullough
authors.forename: Mark
authors.initials: M
authors.lastname: Maxham
authors.forename: Kevin
authors.initials: K
authors.lastname: Hester
authors.forename: Paul
authors.initials: P
authors.lastname: McNitt
authors.forename: Donald M
authors.initials: DM
authors.lastname: Grey
authors.forename: Carlos
authors.initials: C
authors.lastname: Henriquez
authors.forename: Mathieu
authors.initials: M
authors.lastname: Foquet
authors.forename: Stephen W
authors.initials: SW
authors.lastname: Turner
authors.forename: Denis
authors.initials: D
authors.lastname: Zaccarin
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods; D036103:Nanotechnology
chemical_list: D005456:Fluorescent Dyes