title: C-terminus of TRAP in Staphylococcus can enhance the activity of lysozyme and lysostaphin.
abstract: In Staphylococcus aureus, the target of RNAIII activating protein (TRAP) is a membrane-associated protein whose C-terminus can be used as a vaccine to provide protection against staphylococcal infection. Here, we show for the first time by surface plasmon resonance and enzyme-linked immunosorbent assay that TRAP can specifically bind lysozyme and lysostaphin through its C-terminus (amino acids 155-167) and enhance lysozomal activities in vitro. It was also found that the traP mutant strain is more resistant to lysostaphin than wild-type. Our previous data showed that the C-terminus of TRAP might be extracellular. So our results suggested that the C-terminus of TRAP could act as the specific targeting protein of the lysozyme/lysostaphin on the S. aureus cell wall and the biological significance of the interaction might be to facilitate lysozyme/lysostaphin-mediated cell lysis.
authors.affiliation: Beijing Institute of Basic Medical Sciences, Beijing 100850, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Yang
authors.forename: Yaping
authors.initials: Y
authors.lastname: Gao
authors.forename: Jiannan
authors.initials: J
authors.lastname: Feng
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Li
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.forename: Chuan
authors.initials: C
authors.lastname: Liu
authors.forename: Ming
authors.initials: M
authors.lastname: Fan
authors.forename: Beifen
authors.initials: B
authors.lastname: Shen
authors.forename: Ningsheng
authors.initials: N
authors.lastname: Shao
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry*; D001665:Binding Sites; D004789:Enzyme Activation; D008248:Lysostaphin / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D016601:RNA-Binding Proteins / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000378:metabolism*; D014157:Transcription Factors / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; C079092:MtrB protein, Bacteria; D016601:RNA-Binding Proteins; D014157:Transcription Factors; D009113:Muramidase; D008248:Lysostaphin