[Selection and identification of salt-tolerant variants of Taraxacum officinale].
In order to obtain salt-tolerant variant plants of Dandelion (Taraxacum officinale Weber), the leaf discs were excised from 20 to 30-day old seedlings to produce callus, then the induced calli were transferred to selection mediums containing 1.5% NaCl. After regenerating and rooting, these salt-tolerant calli finally developed into 12 variant plantlets. Compared with the wild-type, these regenerated plants produced more trichomes on their leaves, and had larger leaves and shorter petioles. Additionally, the dumpy roots and an approximately 2-cm bract in middle parts of the floricanes were clearly observed in these salt-tolerant plants. By RAPD (Random Amplified Polymorphic DNA) and SDS-PAGE analysis, these salt-tolerant plants showed differences from the control at DNA and protein levels. With 1.5% NaCl treatment, the antioxidant enzyme activity, proline content, and flavonoid concentration were higher in these salt-tolerant plants, whereas maloaldehyde concentration was significantly lower. Salt-tolerant lines of T. officinale showed stronger anti-oxidative activity and higher flavonoid contents.
Zhang
Xinguo
X
Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Li
Yinxin
Y
Chen
Hua
H
Shi
Wuliang
W
D046508:Culture Techniques / Q000379:methods; D004361:Drug Tolerance / Q000235:genetics*; D047309:Flavones / Q000032:analysis; D014644:Genetic Variation / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics / Q000254:growth & development; D019105:Random Amplified Polymorphic DNA Technique; D055051:Salt-Tolerant Plants / Q000235:genetics / Q000254:growth & development; D036226:Seedlings / Q000235:genetics / Q000254:growth & development; D012965:Sodium Chloride / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000032:analysis; D031205:Taraxacum / Q000235:genetics* / Q000254:growth & development*
D047309:Flavones; D012965:Sodium Chloride; D013482:Superoxide Dismutase