Synthesis and spectroscopic DNA binding studies of [Ru(phen)2(tbtc)]2+ and [Ru(2,9-dmp)2(tbtc)]2+.
A new polypyridyl ligand tbtc (tbtc=4,5,9,14-tetraaza-benzo[b]triphenylene-11-carboxylic acid methyl ester) and its complexes [Ru(phen)2(tbtc)]2+ (1) (phen=1,10-phenanthroline) and [Ru(2,9-dmp)2(tbtc)]2+ (2) (2,9-dmp=2,9-dimethyl-1,10-phenanthroline) were synthesized and characterized by element analysis, MS, and 1H NMR. The DNA binding properties of both complexes to calf thymus DNA (CT-DNA) were investigated by different spectrophotometric methods and viscosity measurements. The results suggest that both complexes bind to DNA via an intercalative mode, and the DNA binding affinity of complex 1 is much greater than that of complex 2. This difference in binding affinity probably was caused by the different ancillary ligands. Also, when irradiated at 365 nm, complex 1 was found to be a more-effective DNA-cleaving agent than complex 2.
Tan
Li-Feng
LF
College of Chemistry, Xiangtan University, Xiangtan 411105, PR China. lfwyxh@yahoo.com.cn
Wang
Fang
F
Su
Guo-Jun
GJ
Chao
Hui
H
Zhang
Sheng
S
D000818:Animals; D002417:Cattle; D002621:Chemistry / Q000379:methods; D004247:DNA / Q000737:chemistry*; D008024:Ligands; D008027:Light; D009682:Magnetic Resonance Spectroscopy; D008956:Models, Chemical; D010618:Phenanthrolines / Q000138:chemical synthesis* / Q000737:chemistry*; D010957:Plasmids / Q000378:metabolism; D013053:Spectrophotometry / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014783:Viscosity
D008024:Ligands; D010618:Phenanthrolines; D004247:DNA; C427203:calf thymus DNA