title: Halomonas caseinilytica sp. nov., a halophilic bacterium isolated from a saline lake on the Qinghai-Tibet Plateau, China.
abstract: A halophilic, Gram-negative bacterial strain, designated AJ261T, which was isolated from a soil sample from a salt lake on the Qinghai-Tibet Plateau, was subjected to a polyphasic taxonomic study. The isolate grew optimally in the presence of 3-5 % NaCl and used various carbohydrates as sole carbon and energy sources. The genomic DNA G+C content was 63.0 mol%. The predominant fatty acids were C18 : 1omega7c, C16 : 0 and C12 : 0. A phylogenetic analysis based on 16S rRNA gene sequences indicated that the isolate had the highest sequence similarity with respect to type strains of Halomonas elongata (98.2 %), Halomonas eurihalina (98.1 %) and Halomonas halmophila (97.2 %). The DNA-DNA relatedness of strain AJ261T with respect to H. elongata NBRC 15536T, H. eurihalina CGMCC 1.2318T and H. halmophila DSM 5349T was 42, 25 and 26 %, respectively. Overall, the phenotypic, genotypic and phylogenetic results demonstrate that strain AJ261T represents a novel species within the genus Halomonas, for which the name Halomonas caseinilytica is proposed. The type strain is AJ261T (=CGMCC 1.6773T =JCM 14802T).
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
authors.forename: Yue-Hong
authors.initials: YH
authors.lastname: Wu
authors.forename: Xue-Wei
authors.initials: XW
authors.lastname: Xu
authors.forename: Ying-Yi
authors.initials: YY
authors.lastname: Huo
authors.forename: Peng
authors.initials: P
authors.lastname: Zhou
authors.forename: Xu-Fen
authors.initials: XF
authors.lastname: Zhu
authors.forename: Hui-Bin
authors.initials: HB
authors.lastname: Zhang
authors.forename: Min
authors.initials: M
authors.lastname: Wu
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D020459:Genes, rRNA; D005838:Genotype; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012965:Sodium Chloride