title: Plasma polymerized epoxide functional surfaces for DNA probe immobilization.
abstract: The development of functional surfaces for the immobilization of DNA probe is crucial for a successful design of a DNA sensor. In this report, epoxide functional thin films were achieved simply by pulsed plasma polymerization (PP) of glycidyl methacrylate (GMA) at low duty cycle. The presence of epoxide groups in the resulting ppGMA films was confirmed by Fourier transform infrared spectroscopy. The ppGMA coatings were found to be resistant to the non-specific adsorption of DNA strands, while the epoxide groups obtained could react with amine-modified DNA probes in a mild basic environment without any activation steps. A DNA sensor was made, and was successfully employed to distinguish different DNA sequences with one base pair mismatch as seen by surface plasmon enhanced fluorescence spectroscopy (SPFS). The regeneration of the present DNA sensor was also discussed. This result suggests that surface modification with ppGMA films is very promising for the fabrication of various DNA sensors.
authors.affiliation: Max-Planck-Institut für Polymerforschung, Mainz, Germany. chuliqiang@nd.edu
authors.forename: Li-Qiang
authors.initials: LQ
authors.lastname: Chu
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Knoll
authors.forename: Renate
authors.initials: R
authors.lastname: Förch
mesh_terms: D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004852:Epoxy Compounds / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004852:Epoxy Compounds; D008689:Methacrylates; D011108:Polymers; D004247:DNA; C007870:glycidyl methacrylate