L-arginine protects from pringle manoeuvere of ischemia-reperfusion induced liver injury.
Pringle described a new technique to reduce blood loss during liver surgery. Adult Wistar rats were subjected to 1 h of partial liver ischemia and followed by 3 h reperfusion. Eighteen Wistar rats were divided into sham-operated control group (I) (n=6), ischemia and reperfusion (I/R) group (II) (n=6), L-arginine treated group (100 mg/kg body weight/daily by oral route for 7 d before induced ischemia reperfusion maneuver) (III) (n=6). Ischemic and reperfusion hepatocellular injury occurred as indicated by increased-alanine transaminase (ALT), aspartate transaminase (AST). Pre-treatment with L-arginine significantly decreased serum-ALT, AST after 1 h ischemia followed by 3 h of reperfusion. Nitric oxide production, in hepatocytes was increased 2 fold and MDA levels significantly decreased by L-arginine treatment as compared to I/R rat. Histopathology and TEM studies showed markedly diminished hepatocellular injury in L-arginine pretreated rats during the hepatic I/R, which reached a level comparable to saline-treated rat of sham operated group. Thus, findings it may be concluded that L-arginine afforded significant protection from hepatobiliary function from I/R injury by nitric oxide production.
Chattopadhyay
Pronobesh
P
College of Pharmacy IFTM, Lodhipur RajputMoradabad-244001 UP, India. chatto_pronobesh@rediffmail.com
Verma
Navneet
N
Verma
Anurag
A
Kamboj
Tirath
T
Khan
Nazam Ali
NA
Wahi
Arun Kumar
AK
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001120:Arginine / Q000494:pharmacology*; D001219:Aspartate Aminotransferases / Q000097:blood; D016923:Cell Death / Q000187:drug effects; D003250:Constriction; D006499:Hepatic Artery / Q000187:drug effects; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D008107:Liver Diseases / Q000473:pathology / Q000517:prevention & control*; D008315:Malondialdehyde / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D009569:Nitric Oxide / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D014664:Vasodilation / Q000187:drug effects
D009569:Nitric Oxide; D008315:Malondialdehyde; D001120:Arginine; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase