Two new steroidal compounds from starfish Asterias amurensis Lutken.
Two new sulfated steroidal compounds (1 and 2), along with three known steroidal saponins (3, 4, and 5) were isolated from the starfish Asterias amurensis Lutken. The structures of new compounds were elucidated as 3beta-O-sulfated-cholest-5-ene-7alpha-ol (1) and (E) 25-O-beta-d-xylopyranosyl-26, 27-dinor-24(S)-methyl-22-ene-15alpha-O-sulfated-5alpha-cholest-3beta,6alpha-ol (2) by extensive NMR experiments and chemical evidence. Their effects on UMR106 cell proliferation were screened by MTT method. The results indicated that compounds 2 and 2a (0.01-100 microM) significantly promoted the osteoblastic proliferation. The initial structure-activity relationship analysis suggests that the sugar moiety is the necessary group for the activity.
Liu
Hong-Wei
HW
School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing, China. liuhongwei60@yahoo.com.cn
Li
Jian-Kuan
JK
Zhang
Da-Wei
DW
Zhang
Jing-Chao
JC
Wang
Nai-Li
NL
Cai
Guo-Ping
GP
Yao
Xin-Sheng
XS
D000818:Animals; D047271:Asterias / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D008968:Molecular Conformation; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology
D012503:Saponins; D013256:Steroids