Processing of the circumsporozoite protein in infected hepatocytes is not dependent on aspartic proteases.
CD8(+) T cells play a major role in the protective immune response against the liver stage of malaria. It was previously shown that the circumsporozoite protein (CSP) is processed and presented to specific T cells by both traversed and infected hepatocytes, but their respective antigen processing requirements were not completely defined. In the present study, we show that in vitro processing of the Plasmodium berghei CSP by infected mouse primary hepatocytes is exclusively dependent on proteasomes, while aspartic proteases are also needed in the case of traversed hepatocytes.
Bongfen
S E
SE
Department of Biochemistry, University of Lausanne, Ch. des Boveresses, Epalinges, Switzerland.
Balam
S
S
Torgler
R
R
Romero
J F
JF
Corradin
G
G
D000818:Animals; D017951:Antigen Presentation; D000953:Antigens, Protozoan / Q000276:immunology*; D016282:Aspartic Acid Endopeptidases / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D018920:Coculture Techniques; D022781:Hepatocytes / Q000276:immunology* / Q000469:parasitology; D008288:Malaria / Q000276:immunology* / Q000469:parasitology; D051379:Mice; D008807:Mice, Inbred BALB C; D010962:Plasmodium berghei / Q000276:immunology*; D046988:Proteasome Endopeptidase Complex / Q000502:physiology; D015800:Protozoan Proteins / Q000276:immunology*; D034101:Sporozoites / Q000276:immunology
D000953:Antigens, Protozoan; D015800:Protozoan Proteins; C041595:circumsporozoite protein, Protozoan; D016282:Aspartic Acid Endopeptidases; D046988:Proteasome Endopeptidase Complex