title: Separation of chlorogenic acid from honeysuckle crude extracts by macroporous resins.
abstract: Chlorogenic acid, one of the most bioactive compounds rich in the Chinese medicinal herb honeysuckle, is a natural antioxidant and serves as anti-inflammatory, anti-tumor, anti-mutagenic and anti-carcinogenic agent. An efficient preparative separation process of chlorogenic acid from honeysuckle crude extracts has been developed in the present study. HPD-850 resin offers the best adsorption capacity, and adsorption and desorption ratios for chlorogenic acid among the nine macroporous resins tested, and its adsorption rate at 25 degrees C fit best to the Langmuir isotherm. The adsorption capacity of HPD-850 resin was found to depend strongly on the pH value of the initial adsorption solution. The dynamic adsorption and desorption experiments have been carried out on a HPD-850 resin packed column to optimize the separation process of chlorogenic acid from honeysuckle crude extracts. After one run treatment with HPD-850 resin, the chlorogenic acid content in the final product was increased 4.46-fold from 11.2% to 50.0%, with a recovery yield of 87.9%. The preparative separation of chlorogenic acid can be easily and efficiently achieved via adsorption and desorption on HPD-850 resin, and the method developed will provide a potential approach for large-scale separation and purification of chlorogenic acid for its wide pharmaceutical use.
authors.affiliation: National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.forename: Ruiyuan
authors.initials: R
authors.lastname: Yang
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhao
authors.forename: Chun-Zhao
authors.initials: CZ
authors.lastname: Liu
mesh_terms: D000327:Adsorption; D002726:Chlorogenic Acid / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D031239:Lonicera / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D010936:Plant Extracts; D002726:Chlorogenic Acid