title: Next-generation high-density self-assembling functional protein arrays.
abstract: We developed a high-density self-assembling protein microarray, based on the nucleic acid programmable protein array (NAPPA) concept, to display thousands of proteins that are produced and captured in situ from immobilized cDNA templates. We arrayed up to 1,000 unique human cDNAs and obtained high yields of protein expression and capture with minimal variation and good reproducibility. This method will enable various experimental approaches to study protein function in high throughput.
authors.affiliation: Harvard Institute of Proteomics, Harvard Medical School, 320 Charles Street, Cambridge, Massachusetts 02141, USA.
authors.forename: Niroshan
authors.initials: N
authors.lastname: Ramachandran
authors.forename: Jacob V
authors.initials: JV
authors.lastname: Raphael
authors.forename: Eugenie
authors.initials: E
authors.lastname: Hainsworth
authors.forename: Gokhan
authors.initials: G
authors.lastname: Demirkan
authors.forename: Manuel G
authors.initials: MG
authors.lastname: Fuentes
authors.forename: Andreas
authors.initials: A
authors.lastname: Rolfs
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Hu
authors.forename: Joshua
authors.initials: J
authors.lastname: LaBaer
mesh_terms: D000418:Albumins / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002474:Cell-Free System; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000378:metabolism; D005456:Fluorescent Dyes / Q000494:pharmacology; D020869:Gene Expression Profiling / Q000295:instrumentation / Q000379:methods*; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D019151:Peptide Library; D040081:Protein Array Analysis / Q000295:instrumentation* / Q000379:methods*; D040901:Proteomics / Q000379:methods* / Q000639:trends
chemical_list: D000418:Albumins; D018076:DNA, Complementary; D005456:Fluorescent Dyes; D019151:Peptide Library; D005982:Glutathione Transferase