Nucleic acid sensor for insecticide detection.
Nucleic acid sensor based on polyaniline (PANI) has been fabricated by covalently immobilizing double stranded calf thymus (dsCT) DNA onto perchlorate (ClO(-) (4))-doped PANI film deposited onto indium-tin-oxide (ITO) glass plate using 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) chemistry. These dsCT-DNA-PANI-ClO(4)/ITO and PANI-ClO(4)/ITO electrodes have been characterized using square wave voltammetry, electrochemical impedance, scanning electron microscopy (SEM) and Fourier-transform-infrared (FTIR) measurements. This disposable dsCT-DNA-PANI-ClO(4)/ITO bioelectrode, stable for about 4 months, can be used to detect cypermethrin (0.005 ppm) and trichlorfon (0.01 ppm) in 30 and 60 s, respectively.
Solanki
Pratima R
PR
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr K. S. Krishnan Road, New Delhi-110012, India.
Prabhakar
Nirmal
N
Pandey
M K
MK
Malhotra
B D
BD
D000814:Aniline Compounds; D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D004247:DNA; D004563:Electrochemistry; D007306:Insecticides / Q000032:analysis*; D008855:Microscopy, Electron, Scanning; D011722:Pyrethrins / Q000032:analysis; D013053:Spectrophotometry; D017550:Spectroscopy, Fourier Transform Infrared; D017971:Tin Compounds; D014236:Trichlorfon / Q000032:analysis
D000814:Aniline Compounds; D007306:Insecticides; D011722:Pyrethrins; D017971:Tin Compounds; C416807:polyaniline; C017160:cypermethrin; C109984:indium tin oxide; D004247:DNA; D014236:Trichlorfon