The L1-CAM, Neuroglian, functions in glial cells for Drosophila antennal lobe development.
Although considerable progress has been made in understanding the roles of olfactory receptor neurons (ORNs) and projection neurons (PNs) in Drosophila antennal lobe (AL) development, the roles of glia have remained largely mysterious. Here, we show that during Drosophila metamorphosis, a population of midline glial cells in the brain undergoes extensive cellular remodeling and is closely associated with the collateral branches of ORN axons. These glial cells are required for ORN axons to project across the midline and establish the contralateral wiring in the ALs. We find that Neuroglian (Nrg), the Drosophila homolog of the vertebrate cell adhesion molecule, L1, is expressed and functions in the midline glial cells to regulate their proper development. Loss of Nrg causes the disruption in glial morphology and the agenesis of the antennal commissural tract. Our genetic analysis further demonstrates that the functions of Nrg in the midline glia require its ankyrin-binding motif. We propose that Nrg is an important regulator of glial morphogenesis and axon guidance in AL development.
Chen
Weitao
W
Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Hing
Huey
H
D000818:Animals; D001369:Axons / Q000378:metabolism; D001921:Brain / Q000254:growth & development* / Q000378:metabolism; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism / Q000502:physiology; D002454:Cell Differentiation / Q000502:physiology; D022081:Cell Surface Extensions / Q000378:metabolism / Q000502:physiology; D004330:Drosophila / Q000254:growth & development* / Q000378:metabolism; D029721:Drosophila Proteins / Q000378:metabolism / Q000502:physiology; D007150:Immunohistochemistry; D039842:Neural Cell Adhesion Molecule L1 / Q000378:metabolism / Q000502:physiology*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D009832:Olfactory Nerve / Q000378:metabolism / Q000502:physiology; D018034:Olfactory Receptor Neurons / Q000166:cytology / Q000378:metabolism / Q000502:physiology
D015816:Cell Adhesion Molecules, Neuronal; D029721:Drosophila Proteins; D039842:Neural Cell Adhesion Molecule L1; C061745:Nrg protein, Drosophila