Transcript profiling of a MAP kinase pathway in C. albicans.
In Candida albicans, a MAP kinase pathway has been implicated in aspects of controlling hyphal development. We have examined the transcription profile of cells deleted for the transcription factor Cph1p as well as Cst20p, Hst7p, and Cek1p, three upstream kinases potentially involved in Cph1p regulation. Deletion of any of these four elements does not block filament induction by serum and does not dramatically affect the transcription profile of the yeast-hyphal transition, but deletion of CPH1 delays filamentation. Over-expression of Cph1p by ADH1pt-CPH1 significantly enhances filamentation, suggesting that Cph1p is helpful but not essential for filament induction. Interestingly, the transcription profile of ADH1pt-CPH1 expressing cells under yeast conditions is similar to that of wild-type strains undergoing the yeast-hyphal transition. Finally, it appears that Cek1 and its regulators Hst7p and Cst20p may control the repression of genes such as CHT2 through a process independent of the Cph1p transcription factor.
Huang
Hao
H
Biotechnology Research Institute, National Research Council, Montreal, PQ, Canada. ecnuhh@yahoo.com <ecnuhh@yahoo.com>
Harcus
Doreen
D
Whiteway
Malcolm
M
D002176:Candida albicans / Q000201:enzymology* / Q000235:genetics / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics; D017353:Gene Deletion; D020869:Gene Expression Profiling; D025301:Hyphae / Q000254:growth & development; D020935:MAP Kinase Signaling System; D048052:Mitogen-Activated Protein Kinase 3 / Q000235:genetics; D020929:Mitogen-Activated Protein Kinase Kinases / Q000235:genetics; D020928:Mitogen-Activated Protein Kinases / Q000096:biosynthesis / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011494:Protein Kinases / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics
C090837:CPH1 protein, Candida albicans; D005656:Fungal Proteins; D014157:Transcription Factors; C077339:CEK1 protein, Candida albicans; D011494:Protein Kinases; C119859:CST20 protein, Candida albicans; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; C119846:HST7 protein, Candida albicans; D020929:Mitogen-Activated Protein Kinase Kinases