title: Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay.
abstract: NMO-IgG, a disease-specific autoantibody for neuromyelitis optica, recognizes aquaporin-4 (AQP4) and has been examined by indirect immunofluorescence assay. We developed an enzyme-linked immunosorbent assay (ELISA) to detect anti-AQP4 antibodies by establishing methods for expression in a baculovirus system and purification of recombinant AQP4 as antigen. Elevated anti-AQP4 antibody titers in serum were found in 15 (71%) of 21 patients with neuromyelitis optica, 4.3% of 46 patients with multiple sclerosis, none of 51 normal controls, and 2.6% of 115 patients with other neurological diseases. The ELISA system can be substituted for the conventional NMO-IgG assay.
authors.affiliation: Department of Neurology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chou-ku, Chiba 260-8670, Japan.
authors.forename: Sei
authors.initials: S
authors.lastname: Hayakawa
authors.forename: Masahiro
authors.initials: M
authors.lastname: Mori
authors.forename: Akiko
authors.initials: A
authors.lastname: Okuta
authors.forename: Akiko
authors.initials: A
authors.lastname: Kamegawa
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Fujiyoshi
authors.forename: Yasumasa
authors.initials: Y
authors.lastname: Yoshiyama
authors.forename: Kaoru
authors.initials: K
authors.lastname: Mitsuoka
authors.forename: Kenichi
authors.initials: K
authors.lastname: Ishibashi
authors.forename: Sei
authors.initials: S
authors.lastname: Sasaki
authors.forename: Takamichi
authors.initials: T
authors.lastname: Hattori
authors.forename: Satoshi
authors.initials: S
authors.lastname: Kuwabara
mesh_terms: D000328:Adult; D051401:Aquaporin 4 / Q000276:immunology*; D001323:Autoantibodies / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000276:immunology; D009471:Neuromyelitis Optica / Q000097:blood* / Q000276:immunology*
chemical_list: D051401:Aquaporin 4; D001323:Autoantibodies; D007074:Immunoglobulin G