Immunohistochemical analysis of the patterns of p53 and PCNA expression in odontogenic cystic lesions.
OBJECTIVE
the role of p53 expression in odontogenic lesions has not been fully determined, but has been associated with cell proliferation. The purpose of this study was to analyze p53 and proliferating cell nuclear antigen (PCNA) expression in 4 different odontogenic lesions.


METHODS
expression of p53 and PCNA was analyzed in radicular and dentigerous cysts, odontogenic keratocysts, and calcifying odontogenic cysts (Gorlin cysts) using monoclonal antibodies for detection of p53 and PCNA.


RESULTS
PCNA expression was significantly greater in the basal layer of radicular cysts and in the suprabasal layer of odontogenic keratocysts; the percentage of p53 positive cells was significantly greater in the suprabasal layer of odontogenic keratocysts.


CONCLUSIONS
The patterns of p53 and PCNA expression in dentigerous and radicular cysts were similar although the two lesions are of different origin. In odontogenic keratocysts and Gorlin cysts, results indicate a different pattern of tumor growth.
de Oliveira
Márcia Gaiger
MG
Faculdade de Odontologia, Universidade do Rio Grande do Sul, Porto Alegre, RS, Brazil. marciago@gmail.com
Lauxen
Isabel da Silva
Ida S
Chaves
Anna Cecília Moraes
AC
Rados
Pantelis Varvaki
PV
Sant'Ana Filho
Manoel
M
D006801:Humans; D007150:Immunohistochemistry; D009807:Odontogenic Cysts / Q000276:immunology* / Q000473:pathology*; D018809:Proliferating Cell Nuclear Antigen / Q000032:analysis / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000032:analysis / Q000096:biosynthesis*
D018809:Proliferating Cell Nuclear Antigen; D016159:Tumor Suppressor Protein p53