title: Effect of heparin on production of transforming growth factor (TGF)-beta1 and TGF-beta1 mRNA expression by human normal skin and hyperplastic scar fibroblasts.
abstract: Heparin affects both dermal fibroblast proliferation and collagen and may mediate these effects by altering the levels of transforming growth factor-beta1 (TGF-beta1) production and TGF-beta1 mRNA expression as a wound healing modulator. The purpose of this study is to probe the effect of heparin on TGF-beta1 and TGF-beta1 mRNA production by human normal skin and hyperplastic scar fibroblasts. This research investigates the effect of heparin on TGF-beta1 and TGF-beta1 mRNA production by human normal skin and hyperplastic scar fibroblasts with exposure to 0 microg/mL, 100 microg/mL, 300 microg/mL, or 600 microg/mL heparin for 24, 48, 72, or 96 hours in a serum-free in vitro model. Levels of TGF-beta1 in the supernatants and TGF-beta1 mRNA expression of fibroblasts were determined by enzyme-linked immunosorbent assay (ELISA) and real time RT-PCR, respectively. Heparin (300 microg/mL and 600 microg/mL) stimulated TGF-beta1 production by normal skin (26% to 83%) and hyperplastic scar fibroblasts (63% to 85%), with statistical significance (P < 0.05) at various time points. Heparin (300 microg/mL and 600 microg/mL) also stimulated TGF-beta1 mRNA expression by normal skin (12% to 53%) and hyperplastic scar fibroblasts (33% to 52%), with statistical significance (P < 0.05) at various time points. These effects of heparin on normal skin and hyperplastic scar fibroblasts may have implications for hyperplastic scar formation and wound healing in vivo.
authors.affiliation: Department of Burns and Plastic Surgery, The First Hospital of Xiamen Affiliated to Fujian Medical University, Fujian Province, Xiamen City, China. fsq6603@sina.com
authors.forename: Shu-Qiang
authors.initials: SQ
authors.lastname: Fan
authors.forename: Jing-Long
authors.initials: JL
authors.lastname: Cai
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Qin
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Wang
authors.forename: Zhen-Zhong
authors.initials: ZZ
authors.lastname: Liu
authors.forename: Ming-Liang
authors.initials: ML
authors.lastname: Sun
mesh_terms: D002056:Burns / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D017439:Cicatrix, Hypertrophic / Q000378:metabolism* / Q000473:pathology; D018076:DNA, Complementary / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay; D005343:Fibrinolytic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism*; D006493:Heparin / Q000008:administration & dosage / Q000494:pharmacology*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000187:drug effects* / Q000378:metabolism* / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis / Q000187:drug effects* / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D005343:Fibrinolytic Agents; D012333:RNA, Messenger; D053773:Transforming Growth Factor beta1; D006493:Heparin