title: Overexpression of OgPAE1 from wild rice confers fungal resistance against Botrytis cinerea.
abstract: A full-length cDNA of the OgPAE1 gene encoding the alpha5 subunit of the 20S proteasome was isolated from wild rice (Oryza grandiglumis) treated by wounding or with a fungal elicitor. The deduced amino acid sequence of OgPAE1 comprises 237 amino acids (25.99 kDa), and shows 94.5% homology with Arabidopsis thaliana AtPAE1. Expression of OgPAE1 is regulated by defense-related signaling chemicals such as cantharidin, endothall and jasmonic acid. Overexpression of OgPAE1 in A. thaliana leads to resistance to the fungal pathogen Botrytis cinerea by lowering disease rate and size of necrotic lesions, and by less penetration and colonization of fungal hyphae. The results indicate that the 20S proteasome from wild rice is involved in the B. cinerea defense pathway via an as yet undetermined mechanism.
authors.affiliation: Life Science and Natural Resources, Dong-A University, 840 Hadan-dong, Busan 604-714, Korea.
authors.forename: Eun Hee
authors.initials: EH
authors.lastname: Jeon
authors.forename: Eun Sook
authors.initials: ES
authors.lastname: Chung
authors.forename: Jung Hun
authors.initials: JH
authors.lastname: Pak
authors.forename: Jae Sung
authors.initials: JS
authors.lastname: Nam
authors.forename: Sung Ki
authors.initials: SK
authors.lastname: Cho
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Shin
authors.forename: Doh Hoon
authors.initials: DH
authors.lastname: Kim
authors.forename: Gyung Tae
authors.initials: GT
authors.lastname: Kim
authors.forename: Jai Heon
authors.initials: JH
authors.lastname: Lee
authors.forename: Kyung Ho
authors.initials: KH
authors.lastname: Kang
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Chung
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology; D020171:Botrytis / Q000502:physiology*; D018506:Gene Expression Regulation, Plant / Q000276:immunology*; D017343:Genes, Plant; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021122:Protein Subunits
chemical_list: D010940:Plant Proteins; D021122:Protein Subunits