title: Complement split products and proinflammatory cytokines in intraoperatively salvaged unwashed blood during hip replacement: comparison between heparin-coated and non-heparin-coated autotransfusion systems.
abstract: OBJECTIVE
  The aim of the present study was to investigate the quality of shed blood collected in a new intraoperative autotransfusion system (Sangvia, AstraTech, Sweden) and to study whether heparin-coated surfaces in the device reduce the production of inflammatory mediators.
  
  
  METHODS
  The study was randomized and prospective. Twelve total hip arthroplasty patients whose blood was collected with a device having a heparin-coated surface and 12 patients whose blood was collected with a device having a non-heparin-coated surface were included. Venous blood was drawn from the patients preoperatively. Intraoperatively 200 ml salvaged blood was collected and samples were also withdrawn; samples were obtained from the blood bag.
  
  
  RESULTS
  Compared to venous blood, elevated concentrations of interleukin 6 (IL-6), IL-8, C3a and polymorphonuclear elastase were found in collected blood. No significant differences in inflammatory mediators were found between the heparin-coated and the non-heparin-coated groups. The median haemoglobin concentration in the salvaged blood was 74 g/l in both groups. Plasma haemoglobin and potassium concentrations were also elevated. There were no significant differences between the groups.
  
  
  CONCLUSIONS
  The present study indicates that the blood salvaged intraoperatively contains elevated levels of complement split product and proinflammatory cytokines and that heparin-coated surfaces of the salvage device do not significantly influence the formation of inflammatory mediators.
authors.affiliation: Department of Anaesthesiology and Intensive Care, Sahlgrenska University Hospital/East, Goteborg, Sweden.
authors.forename: A
authors.initials: A
authors.lastname: Kvarnström
authors.forename: A
authors.initials: A
authors.lastname: Schmidt
authors.forename: M
authors.initials: M
authors.lastname: Tylman
authors.forename: M
authors.initials: M
authors.lastname: Jacobsson
authors.forename: A
authors.initials: A
authors.lastname: Bengtsson
mesh_terms: D019644:Arthroplasty, Replacement, Hip / Q000379:methods*; D016063:Blood Loss, Surgical; D001804:Blood Transfusion, Autologous / Q000295:instrumentation* / Q000592:standards; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D015926:Complement C3a / Q000032:analysis; D016207:Cytokines / Q000097:blood; D006493:Heparin; D006801:Humans; D007430:Intraoperative Care / Q000379:methods*
chemical_list: D020099:Coated Materials, Biocompatible; D016207:Cytokines; D015926:Complement C3a; D006493:Heparin