title: Neutralizing antibody blocks adenovirus infection by arresting microtubule-dependent cytoplasmic transport.
abstract: Neutralizing antibodies are commonly elicited by viral infection. Most antibodies that have been characterized block early stages of virus entry that occur before membrane penetration, whereas inhibition of late stages in entry that occurs after membrane penetration has been poorly characterized. Here we provide evidence that the neutralizing antihexon monoclonal antibody 9C12 inhibits adenovirus infection by blocking microtubule-dependent translocation of the virus to the microtubule-organizing center following endosome penetration. These studies identify a previously undescribed mechanism by which neutralizing antibodies block virus infection, a situation that may be relevant for other nonenveloped viruses that use microtubule-dependent transport during cell entry.
authors.affiliation: Department of Immunology and Microbial Science, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA.
authors.forename: Jason G
authors.initials: JG
authors.lastname: Smith
authors.forename: Aurelia
authors.initials: A
authors.lastname: Cassany
authors.forename: Larry
authors.initials: L
authors.lastname: Gerace
authors.forename: Robert
authors.initials: R
authors.lastname: Ralston
authors.forename: Glen R
authors.initials: GR
authors.lastname: Nemerow
mesh_terms: D000258:Adenovirus Infections, Human / Q000276:immunology / Q000503:physiopathology*; D000911:Antibodies, Monoclonal / Q000276:immunology* / Q000378:metabolism; D000914:Antibodies, Viral / Q000276:immunology* / Q000378:metabolism; D001693:Biological Transport, Active / Q000502:physiology; D036022:Capsid Proteins / Q000276:immunology* / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D028961:beta Karyopherins / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D028961:beta Karyopherins; C018315:hexon capsid protein, Adenovirus