UPLC-TOF-MS for plant metabolomics: a sequential approach for wound marker analysis in Arabidopsis thaliana.
The model plant Arabidopsis thaliana was studied for the search of new metabolites involved in wound signalling. Diverse LC approaches were considered in terms of efficiency and analysis time and a 7-min gradient on a UPLC-TOF-MS system with a short column was chosen for metabolite fingerprinting. This screening step was designed to allow the comparison of a high number of samples over a wide range of time points after stress induction in positive and negative ionisation modes. Thanks to data treatment, clear discrimination was obtained, providing lists of potential stress-induced ions. In a second step, the fingerprinting conditions were transferred to longer column, providing a higher peak capacity able to demonstrate the presence of isomers among the highlighted compounds.
Grata
Elia
E
Laboratory of Pharmaceutical Analytical Chemistry, School of Pharmaceutical Sciences, EPGL, University of Geneva, Geneva, Switzerland.
Boccard
Julien
J
Guillarme
Davy
D
Glauser
Gaetan
G
Carrupt
Pierre-Alain
PA
Farmer
Edward E
EE
Wolfender
Jean-Luc
JL
Rudaz
Serge
S
D017360:Arabidopsis / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D019295:Computational Biology / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D010935:Plant Diseases; D010944:Plants / Q000378:metabolism*; D025341:Principal Component Analysis