title: Virus infections in Brazilian honey bees.
abstract: This work describes the first molecular-genetic evidence for viruses in Brazilian honey bee samples. Three different bee viruses, Acute bee paralysis virus (ABPV), Black queen cell virus (BQCV), and Deformed wing virus (DWV) were identified during a screening of RNAs from 1920 individual adult bees collected in a region of southeastern Brazil that has recently shown unusual bee declines. ABPV was detected in 27.1% of colony samples, while BQCV and DWV were found in 37% and 20.3%, respectively. These levels are substantially lower than the frequencies found for these viruses in surveys from other parts of the world. We also developed and validated a multiplex RT-PCR assay for the simultaneous detection of ABPV, BQCV, and DWV in Brazil.
authors.affiliation: Agência Paulista de Tecnologia dos Agronegócios, SAA-SP, Av. Prof. Manoel Cesar Ribeiro, Pindamonhangaba, São Paulo, Brazil. erica@aptaregional.sp.gov.br
authors.forename: Erica Weinstein
authors.initials: EW
authors.lastname: Teixeira
authors.forename: Yanping
authors.initials: Y
authors.lastname: Chen
authors.forename: Dejair
authors.initials: D
authors.lastname: Message
authors.forename: Jeff
authors.initials: J
authors.lastname: Pettis
authors.forename: Jay D
authors.initials: JD
authors.lastname: Evans
mesh_terms: D000818:Animals; D001516:Bees / Q000821:virology*; D001938:Brazil; D007304:Insect Viruses / Q000235:genetics / Q000302:isolation & purification*; D008403:Mass Screening; D012327:RNA Virus Infections / Q000662:veterinary* / Q000821:virology; D012328:RNA Viruses / Q000235:genetics / Q000302:isolation & purification*; D012367:RNA, Viral / Q000032:analysis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014777:Virus Diseases / Q000662:veterinary* / Q000821:virology
chemical_list: D012367:RNA, Viral