title: Quantitative phase microscopy through differential interference imaging.
abstract: An extension of Nomarski differential interference contrast microscopy enables isotropic linear phase imaging through the combination of phase shifting, two directions of shear, and Fourier space integration using a modified spiral phase transform. We apply this method to simulated and experimentally acquired images of partially absorptive test objects. A direct comparison of the computationally determined phase to the true object phase demonstrates the capabilities of the method. Simulation results predict and confirm results obtained from experimentally acquired images.
authors.affiliation: University of Colorado, Department of Electrical and Computer Engineering, Campus Box 425 UCB Boulder, Colorado 80309-0425, USA. Sharon.king@colorado.edu
authors.forename: Sharon V
authors.initials: SV
authors.lastname: King
authors.forename: Ariel
authors.initials: A
authors.lastname: Libertun
authors.forename: Rafael
authors.initials: R
authors.lastname: Piestun
authors.forename: Carol J
authors.initials: CJ
authors.lastname: Cogswell
authors.forename: Chrysanthe
authors.initials: C
authors.lastname: Preza
mesh_terms: D017076:Computer-Aided Design; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000295:instrumentation*; D012031:Refractometry / Q000295:instrumentation*