title: Atrazine exposure decreases cell proliferation in Chinese Hamster Ovary (CHO-K1) cell line.
abstract: The aim of this study was to determine the toxic effect of atrazine at the ovarian cellular level. Chinese Hamster Ovary (CHO-K1) cell line was used to evaluate the degree of in vitro atrazine cytotoxicity and the morphological changes were followed during the cell death. Application of four bioassays confirmed that atrazine decreases ovarian cell proliferation and IC(50) were determined with each assay after 72 h of exposure. The level of apoptosis in atrazine treated cells was low.
authors.affiliation: Faculty of Food Technology and Biotechnology, University of Zagreb, Pierotti St 6, 10000, Zagreb, Croatia.
authors.forename: I
authors.initials: I
authors.lastname: Kmetic
authors.forename: V
authors.initials: V
authors.lastname: Gaurina Srcek
authors.forename: I
authors.initials: I
authors.lastname: Slivac
authors.forename: B
authors.initials: B
authors.lastname: Simić
authors.forename: Z
authors.initials: Z
authors.lastname: Kniewald
authors.forename: J
authors.initials: J
authors.lastname: Kniewald
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001280:Atrazine / Q000633:toxicity*; D016466:CHO Cells; D049109:Cell Proliferation / Q000187:drug effects*; D004396:Coloring Agents; D006224:Cricetinae; D003412:Cricetulus; D006540:Herbicides / Q000633:toxicity*; D008856:Microscopy, Fluorescence; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology; D009499:Neutral Red; D009930:Organic Chemicals; D013778:Tetrazolium Salts; D013844:Thiazoles; D014343:Trypan Blue
chemical_list: D004396:Coloring Agents; D006540:Herbicides; D009930:Organic Chemicals; D013778:Tetrazolium Salts; D013844:Thiazoles; C069245:kenacid blue; D009499:Neutral Red; C022616:thiazolyl blue; D014343:Trypan Blue; D001280:Atrazine