mAtNOS1 induces apoptosis of human mammary adenocarcinoma cells.
mAtNOS1 is a novel gene recently reported in mammalian genome with functions that are not fully understood. The present study shows that in human mammary adenocarcinoma MCF-7 cells, mAtNOS1 expression increases mitochondrial nitric oxide and calcium. Our study further shows that overexpression of mAtNOS1 induces apoptosis in MCF-7 cells by increasing mitochondrial protein tyrosine nitration and cytochrome c release. The present study suggests a novel function for mAtNOS1 in regulating mitochondrial nitric oxide and calcium and inducing apoptosis of MCF-7 cells.
Parihar
Arti
A
Department of Surgery, Heart and Lung Research Institute, and Institute of Mitochondrial Biology, The Ohio State University, Columbus, Ohio, USA.
Parihar
Mordhwaj S
MS
Chen
Zhonghai
Z
Ghafourifar
Pedram
P
D000230:Adenocarcinoma / Q000378:metabolism / Q000473:pathology*; D017209:Apoptosis; D029681:Arabidopsis Proteins / Q000096:biosynthesis*; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D002118:Calcium / Q000378:metabolism; D045744:Cell Line, Tumor; D045304:Cytochromes c / Q000378:metabolism; D005260:Female; D006801:Humans; D007120:Immunochemistry; D008928:Mitochondria / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000096:biosynthesis*; D014162:Transfection; D014443:Tyrosine / Q000378:metabolism
D029681:Arabidopsis Proteins; D009569:Nitric Oxide; D014443:Tyrosine; D045304:Cytochromes c; C521947:NOA1 protein, Arabidopsis; D019001:Nitric Oxide Synthase; D002118:Calcium