Digital imaging of stem cells by electron microscopy.
This chapter deals with basic techniques of scanning and transmission electron microscopy applicable to stem cell imaging. It is sometimes desirable to characterize the fine structure of embryonic and adult stem cells to supplement the images obtained by phase-contrast and confocal immunofluorescent microscopy to compare with the microstructure of cells and tissues reported in the literature. This would help confirm their true identity whilst defining their surface and internal morphology. The intention is to put a face on stem cells during their differentiation.
Sathananthan
A Henry
AH
Monash Immunology & Stem Cell Laboratories, Monash University, Melbourne, Australia.
Nottola
Stefania A
SA
D053687:Adult Stem Cells / Q000648:ultrastructure*; D053595:Embryonic Stem Cells / Q000648:ultrastructure*; D005455:Fluorescent Antibody Technique / Q000379:methods*; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008855:Microscopy, Electron, Scanning / Q000379:methods*; D046529:Microscopy, Electron, Transmission / Q000379:methods*