title: Following protein kinase acivity by electrochemical means and contact angle measurements.
abstract: The electrochemical analysis of the protein kinase, casein kinase, is accomplished by the voltammetric response of Ag(+) ions associated with the phosphorylated product; the sensing surface is regenerated by the cleavage of the phosphorylated product with alkaline phosphatase, and the phosphorylation/de-phosphorylation processes are monitored by XPS and contact angle measurements.
authors.affiliation: Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wieckowska
authors.forename: Di
authors.initials: D
authors.lastname: Li
authors.forename: Ron
authors.initials: R
authors.lastname: Gill
authors.forename: Itamar
authors.initials: I
authors.lastname: Willner
mesh_terms: D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000737:chemistry* / Q000378:metabolism; D001665:Binding Sites; D047388:Casein Kinases / Q000737:chemistry* / Q000378:metabolism; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism; D010766:Phosphorylation; D012834:Silver / Q000737:chemistry; D013057:Spectrum Analysis; D014965:X-Rays
chemical_list: D009842:Oligopeptides; D012834:Silver; D000255:Adenosine Triphosphate; D047388:Casein Kinases; D000469:Alkaline Phosphatase