Plasma polymerized epoxide functional surfaces for DNA probe immobilization.
The development of functional surfaces for the immobilization of DNA probe is crucial for a successful design of a DNA sensor. In this report, epoxide functional thin films were achieved simply by pulsed plasma polymerization (PP) of glycidyl methacrylate (GMA) at low duty cycle. The presence of epoxide groups in the resulting ppGMA films was confirmed by Fourier transform infrared spectroscopy. The ppGMA coatings were found to be resistant to the non-specific adsorption of DNA strands, while the epoxide groups obtained could react with amine-modified DNA probes in a mild basic environment without any activation steps. A DNA sensor was made, and was successfully employed to distinguish different DNA sequences with one base pair mismatch as seen by surface plasmon enhanced fluorescence spectroscopy (SPFS). The regeneration of the present DNA sensor was also discussed. This result suggests that surface modification with ppGMA films is very promising for the fabrication of various DNA sensors.
Chu
Li-Qiang
LQ
Max-Planck-Institut für Polymerforschung, Mainz, Germany. chuliqiang@nd.edu
Knoll
Wolfgang
W
Förch
Renate
R
D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D004852:Epoxy Compounds / Q000737:chemistry*; D008689:Methacrylates / Q000737:chemistry; D009693:Nucleic Acid Hybridization / Q000379:methods*; D011108:Polymers / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared
D004852:Epoxy Compounds; D008689:Methacrylates; D011108:Polymers; D004247:DNA; C007870:glycidyl methacrylate