Intra-abdominal adhesion formation induces anti-oxidative injury, enhances cell proliferation, and prevents complement-mediated lysis.
Whether the alteration of gene expression is accompanied with intra-abdominal adhesion formation is unclear. The aim of this study was to analyze the dynamic gene expression patterns in an animal model of intra-abdominal adhesion formation. The mRNA was extracted from the jejunums of sham control mice and jejunum-abrading mice at 1, 3, 7, and 14 days postsurgery. The mouse cDNA microarray was used to monitor the dynamic changes of the tested genes and up-regulated and down-regulated genes were calculated. Quantitative real-time RT-PCR, and immunohistochemistry staining were used to confirm the accuracy of microarray results at RNA and protein levels. The top 100 genes with the greatest change across all studied mice groups were identified and 93 of them were correct after sequencing verification. Of the 93 genes, 74 genes were up-regulated and 19 were down-regulated following jejunal abrasion. Gene expressions of complement-mediated lysis, anti-oxidative response, and cell proliferation were significantly induced during adhesion formation. Intra-abdominal adhesion induces several genes to eliminate overfilled complement-mediated lysis, prevent oxidative injuries, and enhance cell proliferation. These findings may provide insights into the pathogenesis of intra-abdominal adhesion formation and might also help to identify some new target genes for specific diagnostic tools and novel therapeutic strategies.
Yu
Sung-Liang
SL
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan.
Singh
Sher
S
Chen
Huei-Wen
HW
Chen
Hsuan-Yu
HY
Chen
Jeremy J W
JJ
Chen
Wen-Jone
WJ
Chen
Han-Shiang
HS
Chen
Shyr-Chyr
SC
D000005:Abdomen; D000818:Animals; D016923:Cell Death / Q000235:genetics / Q000276:immunology; D002455:Cell Division / Q000235:genetics; D003165:Complement System Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D015870:Gene Expression; D007579:Jejunal Diseases / Q000235:genetics* / Q000473:pathology; D007583:Jejunum / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D020411:Oligonucleotide Array Sequence Analysis; D018384:Oxidative Stress / Q000235:genetics*; D000267:Tissue Adhesions / Q000235:genetics
D003165:Complement System Proteins