Role of the DksA-like protein in the pathogenesis and diverse metabolic activity of Campylobacter jejuni.
DksA is well known for its regulatory role in the transcription of rRNA and genes involved in amino acid synthesis in many bacteria. DksA has also been reported to control expression of virulence genes in pathogenic bacteria. Here, we elucidated the roles of a DksA-like protein (CJJ81176_0160, Cj0125c) in the pathogenesis of Campylobacter jejuni. As in other bacteria, transcription of stable RNA was repressed by the DksA-like protein under stress conditions in C. jejuni. Transcriptomic and proteomic analyses of C. jejuni 81-176 and an isogenic mutant lacking the DksA-like protein showed differential expression of many genes involved in amino acid metabolism, iron-related metabolism, and other metabolic reactions. Also, the C. jejuni DksA-like protein mutant exhibited a decreased ability to invade intestinal cells and induce release of interleukin-8 from intestinal cells. These results suggest that the DksA-like protein plays an important regulatory role in diverse metabolic events and the virulence of C. jejuni.
Yun
Jiae
J
Department of Food and Animal Biotechnology, School of Agricultural Biotechnology, Seoul National University, San 56-1, Shillim-dong, Kwanak-gu, Seoul 151-921, Korea.
Jeon
Byeonghwa
B
Barton
Yi-Wen
YW
Plummer
Paul
P
Zhang
Qijing
Q
Ryu
Sangryeol
S
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism* / Q000502:physiology; D016123:Campylobacter jejuni / Q000235:genetics* / Q000378:metabolism* / Q000472:pathogenicity; D002460:Cell Line; D015180:Electrophoresis, Gel, Two-Dimensional; D005296:Ferrous Compounds / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D008969:Molecular Sequence Data; D020411:Oligonucleotide Array Sequence Analysis; D009876:Operon / Q000235:genetics; D020543:Proteome / Q000032:analysis / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D014158:Transcription, Genetic; D014774:Virulence / Q000235:genetics
D001426:Bacterial Proteins; D005296:Ferrous Compounds; D016209:Interleukin-8; D020543:Proteome