Safety and immunogenicity of myxoma virus as a new viral vector for small ruminants.
Myxoma virus (MYXV), a leporide-specific poxvirus, represents an attractive candidate for the generation of safe and non-replicative vaccine vectors for other species. With the aim of developing new recombinant vaccines for ruminants, we evaluated the safety and the immunogenicity of recombinant MYXV in sheep. In vitro studies indicated that ovine primary fibroblasts were not permissive for MYXV and that infection of ovine peripheral blood mononuclear cells occurred at a low rate. Although non-specific activation significantly improved the susceptibility of lymphocytes, MYXV infection remained abortive. Histological and immunohistochemical examination at the inoculation sites revealed the development of an inflammatory process and allowed the detection of sparse infected cells in the dermis. In addition, inoculated sheep developed an antibody response directed against MYXV and the product of the transgene. Overall, these results provide the first line of evidence on the potential of MYXV as a viral vector for ruminants.
Pignolet
Béatrice
B
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
Boullier
Séverine
S
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
Gelfi
Jacqueline
J
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
Bozzetti
Marjorie
M
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
Russo
Pierre
P
AFSSA LERPRA les Templiers, 105 route des Chappes, F-06902 Sophia Antipolis, France.
Foulon
Eliane
E
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
Meyer
Gilles
G
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
Delverdier
Maxence
M
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
Foucras
Gilles
G
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
Bertagnoli
Stéphane
S
Université de Toulouse, ENVT, UMR 1225, F-31076 Toulouse, France.|INRA, UMR 1225, F-31076 Toulouse, France.
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D005347:Fibroblasts / Q000821:virology; D005822:Genetic Vectors / Q000008:administration & dosage / Q000502:physiology*; D017929:Hemorrhagic Disease Virus, Rabbit / Q000276:immunology; D007271:Injections, Intradermal; D007963:Leukocytes, Mononuclear / Q000821:virology; D009233:Myxoma virus / Q000472:pathogenicity / Q000502:physiology*; D011817:Rabbits; D016865:Reassortant Viruses / Q000502:physiology; D012756:Sheep; D012867:Skin / Q000821:virology; D013045:Species Specificity; D014611:Vaccination / Q000379:methods* / Q000662:veterinary; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage; D014774:Virulence; D014779:Virus Replication
D000914:Antibodies, Viral; D000956:Antigens, Viral; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines; C081149:viral protein 60, rabbit hemorrhagic disease virus