Formation of a FGF-2 and calcium phosphate composite layer on a hydroxyapatite ceramic for promoting bone formation.
Fibroblast growth factor-2 (FGF-2) was immobilized on a hydroxyapatite (HAP) ceramic in supersaturated calcium phosphate solution prepared using solutions corresponding to clinically approved infusion fluids. To avoid the risk of FGF-2 denaturation, FGF-2 immobilization was carried out at 25 degrees C. FGF-2 was successfully immobilized on HAP ceramic surfaces by deposition with calcium phosphate to form a FGF-2 and calcium phosphate composite layer. A maximum of 2.72 +/- 0.01 microg cm(-2) of FGF-2 was immobilized in the composite layer formed on the HAP ceramic under the optimum condition. A FGF-2-immobilized HAP ceramic is likely to have the ability to release a sufficient amount of FGF-2 to promote bone formation. FGF-2 released from a FGF-2-immobilized HAP ceramic maintained its biological activity, since the proliferation of fibroblastic NIH3T3 was promoted. Therefore, the FGF-2-immobilized HAP ceramic is expected to be a useful material for promoting new bone formation.
Sogo
Yu
Y
National Institute of Advanced Industrial Science and Technology (AIST), Institute for Human Science and Biomedical Engineering, Central 6, 1-1-1, Higashi, Tsukuba, Ibaraki 305-8566, Japan. yu-sogou@aist.go.jp
Ito
Atsuo
A
Onoguchi
Masahiro
M
Oyane
Ayako
A
Tsurushima
Hideo
H
Ichinose
Noboru
N
D000818:Animals; D001846:Bone Development / Q000187:drug effects*; D018786:Bone Substitutes / Q000008:administration & dosage* / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002516:Ceramics / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000737:chemistry*; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells
D018786:Bone Substitutes; D002130:Calcium Phosphates; D016222:Fibroblast Growth Factor 2; D017886:Durapatite; C020243:calcium phosphate