The ability of HDL to inhibit VCAM-1 expression and oxidized LDL uptake is impaired in renal patients.
OBJECTIVE
This study examines the ability of HDL from hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients to suppress the expression of adhesion molecules in endothelial cells (ICAM-1, VCAM-1) and in monocytes (LFA-1, VLA-4) and to inhibit the uptake of oxidized LDL by macrophages.


METHODS
Gene expression and the uptake of oxidized LDL were determined in 12 HD patients, 12 CAPD patients and 14 healthy volunteers.


RESULTS
HDL from renal patients were less effective than control lipoproteins in reducing VCAM-1 expression. HDL from CAPD patients inhibited LFA-1 expression to the highest extent. The ability of HDL from renal patients to reduce oxidized LDL uptake was lower compared to control group.


CONCLUSIONS
Decreased ability of HDL to suppress expression of VCAM-1 in endothelial cells and the uptake of oxidized LDL by macrophages can be one of the risk factors for atherosclerosis development in patients with renal failure.
Jurek
Aleksandra
A
Department of General Biochemistry, Faculty of Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Cracow, Poland. Aleksandra.Jurek@LICR.uu.se
Turyna
Bohdan
B
Kubit
Piotr
P
Klein
Andrzej
A
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D040881:CD11a Antigen / Q000378:metabolism; D008076:Cholesterol, HDL / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism; D005260:Female; D006801:Humans; D039441:Integrin alpha4 / Q000378:metabolism; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D007676:Kidney Failure, Chronic / Q000378:metabolism* / Q000628:therapy; D008077:Lipoproteins, LDL / Q000378:metabolism*; D016169:Lymphocyte Function-Associated Antigen-1 / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010531:Peritoneal Dialysis, Continuous Ambulatory; D012333:RNA, Messenger / Q000378:metabolism; D006435:Renal Dialysis; D020298:U937 Cells; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism*
D040881:CD11a Antigen; D008076:Cholesterol, HDL; D008077:Lipoproteins, LDL; D016169:Lymphocyte Function-Associated Antigen-1; D012333:RNA, Messenger; D019010:Vascular Cell Adhesion Molecule-1; C099252:oxidized low density lipoprotein; D018799:Intercellular Adhesion Molecule-1; D039441:Integrin alpha4