REG1A expression is a prognostic marker in colorectal cancer and associated with peritoneal carcinomatosis.
By expression profiling of early staged colon carcinomas, we found regenerating islet-derived 1 alpha (REG1A) to be upregulated in patients with an unfavorable clinical outcome. For validation, REG1A expression was quantified in another colorectal cancer (CRC) patient cohort by Taqman PCR. Aside from tumor and normal tissue from 63 nonpretreated CRC patients, 31 mucosa biopsies from healthy individuals as well as 22 adenomas were included in the investigation. REG1A was significantly upregulated in tumor specimens (p < 0.001) and adenoma (p < 0.01) as compared to normal colorectal tissue. REG1A expression in normal peritumoral tissue in turn proved to be significantly elevated compared to mucosa from healthy individuals (p < 0.01). Determination of REG1A expression might be useful for early tumor diagnosis with a sensitivity of 90.6%, and a specificity of 77.9%. REG1A expression was significantly increased in tumors with peritoneal carcinomatosis (p < 0.01). Moreover, REG1A turned out to be a significant predictor of disease-free survival (p < 0.05). In conclusion, we present evidence that REG1A is a molecular marker of prognostic value and is associated with peritoneal carcinomatosis in CRC. REG1A turned out to be already significantly raised in peritumoral normal tissue compared to mucosa from healthy individuals, suggesting a molecular field effect of secreted REG1A.
Astrosini
Christian
C
Clinic of Surgery and Surgical Oncology, Robert Roessle Hospital, Charité Campus Buch, Berlin, Germany.
Roeefzaad
Claudia
C
Clinic of Surgery and Surgical Oncology, Robert Roessle Hospital, Charité Campus Buch, Berlin, Germany.
Dai
Yi-Yang
YY
Clinic of Surgery and Surgical Oncology, Robert Roessle Hospital, Charité Campus Buch, Berlin, Germany.
Dieckgraefe
Brian K
BK
Division of Gastroenterology, Siteman Cancer Center, Washington University School of Medicine, St. Louis, MO.
Jöns
Thomas
T
Institute for Integrative Neuroanatomy, Center for Anatomy, Charité Campus Mitte, Berlin, Germany.
Kemmner
Wolfgang
W
Surgical Oncology Research Group, Max Delbrueck Center for Molecular Medicine, Berlin, Germany.
D014408:Biomarkers, Tumor / Q000378:metabolism*; D002277:Carcinoma / Q000378:metabolism* / Q000556:secondary; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology*; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D053208:Kaplan-Meier Estimate; D051847:Lithostathine / Q000378:metabolism*; D009367:Neoplasm Staging; D020411:Oligonucleotide Array Sequence Analysis; D010534:Peritoneal Neoplasms / Q000378:metabolism* / Q000556:secondary*; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D011379:Prognosis; D015203:Reproducibility of Results; D015854:Up-Regulation
D014408:Biomarkers, Tumor; D051847:Lithostathine; C497617:REG1A protein, human