[Construction of a novel gene therapy lentiviral vector for drug resistant selection and detection in vivo].
Lentiviral vectors were powerful gene delivery tools for gene therapy. We developed a new lentiviral vector pBobi-MIL that constitutively expressed O6-methylguanine-DNAmethyltransferase (MGMT) and Luciferase, linked by the internal ribosomal entry site (IRES), to realize drug tolerance and real time monitoring in vivo. All results from RT-PCR, drug treating clones forming, immunofluorometric assay and chemiluminescence detection showed that cells infected by recombinant lentivirus L-MIL simultaneously expressed these two genes. This lays the foundation for the further research in gene therapy and can also help identify lentivirus titer.
Xu
Chenyu
C
National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Xiamen University, Xiamen 361005, China.
Gu
Ying
Y
Hou
Wangheng
W
Cheng
Tong
T
Zhang
Tao
T
Que
Yuqiong
Y
Gao
Shuangquan
S
Zhang
Jun
J
Han
Jiahuai
J
Xia
Ningshao
N
D015254:DNA Modification Methylases / Q000096:biosynthesis* / Q000235:genetics; D045643:DNA Repair Enzymes / Q000096:biosynthesis* / Q000235:genetics; D004351:Drug Resistance / Q000235:genetics; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D016086:Lentivirus / Q000235:genetics* / Q000378:metabolism; D008156:Luciferases / Q000096:biosynthesis* / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000096:biosynthesis* / Q000235:genetics
D025521:Tumor Suppressor Proteins; D008156:Luciferases; D015254:DNA Modification Methylases; C515491:MGMT protein, human; D045643:DNA Repair Enzymes