title: Cell patch seeding and functional analysis of cellularized scaffolds for tissue engineering.
abstract: Cell seeding has a direct impact on the final structure and function of tissue constructs, especially for applications like tissue engineering and regeneration. In this study seeding cell patches retrieved from the thermoresponsive poly(N-isopropylacrylamide) surface were used to generate in vitro tissue constructs. Porous and dense bone substitute materials were cellularized using osteoblast cells by a patch transfer and a trypsin method. The function and proliferation of cells was analyzed after 7 days of culture. The relative cell growth rate was found to be higher in cellularized porous hydroxyapatite (PHA) than in dense hydroxyapatite. Live-dead staining confirmed viable cells inside the pores of PHA. Increased alkaline phosphatase activity of cells transferred by the cell patch over the trypsin method revealed the significance of cell patch seeding. This novel method of generating tissue constructs by cell patch seeding was successful in cellularizing scaffolds with intact cell function.
authors.affiliation: Division of Implant Biology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala 695012, India.
authors.forename: P R
authors.initials: PR
authors.lastname: Anil Kumar
authors.forename: H K
authors.initials: HK
authors.lastname: Varma
authors.forename: T V
authors.initials: TV
authors.lastname: Kumary
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002470:Cell Survival; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry*; D008422:Materials Testing; D010012:Osteogenesis / Q000502:physiology*; D016062:Porosity
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D006882:Hydroxyapatites