Cutting edge: rapid accumulation of epidermal CCL27 in skin-draining lymph nodes following topical application of a contact sensitizer recruits CCR10-expressing T cells.
CC chemokine receptor 10 and its ligand, CCL27, are important components of T cell-mediated cutaneous immunity, but whether they influence lymph node (LN) homing by T cells is unknown. In this study, CCL27 protein was detected in skin-draining LN by Western blotting and ELISA although CCL27 mRNA transcripts were low. CCL27 protein was present at higher levels in skin-draining LN compared with gut-draining LN and spleen. A single topical treatment of mouse skin with the contact sensitizer 2,4-dinitro-1-fluorobenzene (DNFB) resulted in a 13-fold increase in CCL27 protein accumulation in skin-draining LN within 1 h and a 5-fold elevation in CCR10 mRNA (normalized to the T cell marker CD2) within 6 h. DNFB treatment also resulted in rapid depletion of approximately 75% of CCL27 from the epidermis. In summary, we describe a novel mechanism for the recruitment of CCR10-positive T cells to skin-draining LN following the rapid release of preformed CCL27 from the epidermis.
Huang
Victor
V
Dermatology Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
Lonsdorf
Anke S
AS
Fang
Lei
L
Kakinuma
Takashi
T
Lee
Vivian C
VC
Cha
Emily
E
Zhang
Hong
H
Nagao
Keisuke
K
Zaleska
Marzanna
M
Olszewski
Waldemar L
WL
Hwang
Sam T
ST
D000328:Adult; D000818:Animals; D015153:Blotting, Western; D054425:Chemokine CCL27 / Q000378:metabolism*; D002634:Chemotaxis, Leukocyte / Q000276:immunology; D003877:Dermatitis, Contact / Q000276:immunology*; D004139:Dinitrofluorobenzene / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005455:Fluorescent Antibody Technique; D006801:Humans; D008198:Lymph Nodes / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D012333:RNA, Messenger / Q000032:analysis; D054447:Receptors, CCR10 / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
C119538:Ccl27a protein, mouse; C515707:Ccr10 protein, mouse; D054425:Chemokine CCL27; D012333:RNA, Messenger; D054447:Receptors, CCR10; D004139:Dinitrofluorobenzene