Determination of some phenolic acids in Majorana hortensis by capillary electrophoresis with online electrokinetic preconcentration.
An online accumulation/mobilization preconcentration technique based on a dynamic pH junction technique and electrokinetic injection was employed for analysis of phenolic acids (sinapic, ferulic, coumarinic, caffeic, syringic, vanillic, and 4-hydroxybenzoic acid) in extracts from Majorana hortensis leaves. Samples were extracted by pressurized solvent extraction with acetone at 150 degrees C and 15 MPa. The capillary electrophoretic method employed 50 mmol.L (-1) sodium borate, pH 9.5, as the sample electrolyte, 50 mmol.L (-1) sodium phosphate, pH 2.5, as the background electrolyte, and 50 mmol.L (-1) sodium phosphate, pH 2.5, with 60 mmol.L (-1) sodium dodecyl sulfate as the mobilization electrolyte. The method allowed 720-fold to 5560-fold preconcentration of the phenolic acids during 30 min of electrokinetic accumulation with detection limits from 0.38 to 4.22 ng.mL (-1).
Petr
Jan
J
Department of Analytical Chemistry, Faculty of Science, Palacky University in Olomouc, Trida Svobody 8, Olomouc, Czech Republic. petrjan1@gmail.com
Vítková
Katerina
K
Ranc
Václav
V
Znaleziona
Joanna
J
Maier
Vítezslav
V
Knob
Radim
R
Sevcík
Juraj
J
D001881:Borates; D003373:Coumaric Acids / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D062385:Hydroxybenzoates / Q000032:analysis*; D031341:Origanum / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*
D001881:Borates; D003373:Coumaric Acids; D062385:Hydroxybenzoates; D010936:Plant Extracts; C010634:sodium borate