Peony root extract upregulates transthyretin and phosphoglycerate mutase in mouse cobalt focus seizure.
Cobalt focus is a seizure focus model in which cerebral neurons exhibit long-lasting severe spike discharges, followed by neuronal death. However, the neuronal death is prevented when peony root extract (PR) is administered prior to cobalt application. We tested the hypothesis that PR modulates the expression of neuroprotective proteins in the cerebrum of mouse cobalt focus by proteomic analysis using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry to screen for differentially expressed proteins. Analyses revealed that transthyretin, a carrier protein for thyroid hormones and retinoids, and the brain form of phosphoglycerate mutase, a glycolytic enzyme, were upregulated in the cobalt-treated mouse cerebrum and further increased by PR administration in association with upregulation of neurogranin/RC3, a target of the transcriptional activation by thyroid hormones and retinoids. These findings suggest that PR-induced protection of mouse cerebral neurons involves neurotrophic events caused by thyroid hormones and/or retinoids and enhanced glycolysis.
Kajiwara
Kagemasa
K
Division of Basic Molecular Science and Molecular Medicine, Tokai University School of Medicine Isehara, Kanagawa 259-1193, Japan. kagemasa@is.icc.u-tokai.ac.jp
Sunaga
Katsuyoshi
K
Tsuda
Tadashi
T
Sugaya
Aiko
A
Sugaya
Eiichi
E
Kimura
Minoru
M
D000818:Animals; D000927:Anticonvulsants / Q000008:administration & dosage*; D054022:Cerebrum / Q000187:drug effects* / Q000378:metabolism; D003035:Cobalt / Q000037:antagonists & inhibitors / Q000633:toxicity; D004195:Disease Models, Animal; D015180:Electrophoresis, Gel, Two-Dimensional; D051379:Mice; D008810:Mice, Inbred C57BL; D051579:Neurogranin / Q000032:analysis / Q000378:metabolism; D029596:Paeonia; D010736:Phosphoglycerate Mutase / Q000032:analysis / Q000378:metabolism*; D010936:Plant Extracts / Q000008:administration & dosage*; D018517:Plant Roots; D011228:Prealbumin / Q000032:analysis / Q000378:metabolism*; D040901:Proteomics; D012640:Seizures / Q000139:chemically induced / Q000517:prevention & control*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013963:Thyroid Hormones / Q000378:metabolism; D015854:Up-Regulation
D000927:Anticonvulsants; D010936:Plant Extracts; D011228:Prealbumin; D013963:Thyroid Hormones; D051579:Neurogranin; D003035:Cobalt; D010736:Phosphoglycerate Mutase