The potential influence of fruit polyphenols on colonic microflora and human gut health.
The effect of common dietary polyphenols on growth of human gut bacteria and their adhesion to enterocytes was investigated. The influence on the growth of a probiotic (Lactobacillus rhamnosus), a commensal (Escherichia coli) and two pathogenic bacteria (Staphylococcus aureus, Salmonella typhimurium) was determined, together with effects on adhesion of pathogenic and probiotic bacteria to cultured Caco-2 cells. All polyphenols, except rutin, were found to affect the viability of representative gut flora in vitro, at doses likely to be present in the gastrointestinal tract, but to differing degrees. Naringenin and quercetin were the most active with the lowest minimum inhibitory concentrations for all the four bacteria tested. The remaining polyphenols had the most marked effect on the Gram positive enteropathogen S. aureus. Naringenin and phloridzin were the most effective inhibitors of S. typhimurium adherence to Caco-2 enterocytes while phloridzin and rutin enhanced the adherence of the probiotic L. rhamnosus. Polyphenols appear to have potential to alter gut microecology and, by affecting the total number of beneficial microflora in the gut, may confer positive gut health benefits.
Parkar
Shanthi G
SG
The Horticulture and Food Research Institute of New Zealand Limited, Private Bag 3123, Hamilton, New Zealand. sparkar@hortresearch.co.nz
Stevenson
David E
DE
Skinner
Margot A
MA
D001422:Bacterial Adhesion / Q000187:drug effects* / Q000502:physiology*; D018938:Caco-2 Cells; D003106:Colon / Q000382:microbiology*; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D020895:Enterocytes / Q000382:microbiology*; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D052201:Lacticaseibacillus rhamnosus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D008826:Microbial Sensitivity Tests; D010636:Phenols / Q000494:pharmacology*; D059808:Polyphenols; D019936:Probiotics; D012486:Salmonella typhimurium / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development / Q000502:physiology
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols