title: [Expression of matrix metalloproteinase-2, -9 and their inhibitor-1 in hypertrophic scars].
abstract: OBJECTIVE
  To investigate the gene expression of matrix metalloproteinases (MMP-2, MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in proliferative and mature hypertrophic scars.
  
  
  METHODS
  Total RNA from 8 normal skin samples and from 16 human hypertrophic scar samples of different maturing stage was respectively extracted, and then mRNA was isolated. The gene expressions of MMP-2, MMP-9 and TIMP-1 in these samples were examined with reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  The gray scale ratio of MMP-2, MMP-9 and TIMP-1 transcription in normal skin were (3.8 +/- 0.7)%, (5.8 +/-4.4)%, (30.3 +/- 3.0)%, respectively, which were obviously higher than those in proliferative hypertrophic scar [(14 +/- 5)%, (18 +/- 5)%, (38 +/- 4)%, P < 0.05]. The expression of MMP-2 and MMP-9 genes in mature hypotrophic scar returned to normal level, but that of TIMP-1 remained high when compared with that of normal level (P < 0. 05).
  
  
  CONCLUSIONS
  The increase in MMP-2, MMP-9 and TIMP-1 gene expression might be involved in the formation of hypertrophic scars, while the lowering of MMP-2 and MMP-9 gene expression might be associated with the maturation of hypertrophic scars.
authors.affiliation: Department of Burns and Plastic Surgery, Youanmen Hospital of Beijing Hospital Association, Beijing 100069, PR China.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Xie
authors.forename: Li-Xin
authors.initials: LX
authors.lastname: He
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Hao
authors.forename: Bi
authors.initials: B
authors.lastname: Chen
authors.forename: Chi-Yu
authors.initials: CY
authors.lastname: Jia
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Sun
authors.forename: Yu-Jue
authors.initials: YJ
authors.lastname: Cao
authors.forename: Dong Hai
authors.initials: DH
authors.lastname: Li
mesh_terms: D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015870:Gene Expression; D006801:Humans; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000235:genetics / Q000378:metabolism*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000473:pathology; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D019715:Tissue Inhibitor of Metalloproteinase-1; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9