title: Purification and preparation of compounds from an extract of Scutellaria barbata D. Don using preparative parallel high performance liquid chromatography.
abstract: Preparative parallel high performance liquid chromatography combined with solvent partition and other pretreatments were adopted to separate and purify compounds from an extract of Scutellaria barbata D. Don. Mass-triggered fraction collection allowed the rapid and precise isolation of target compounds. Twelve compounds were isolated from the extract of S. barbata D. Don, their purity in area percent was determined by HPLC analysis, and the structures of seven compounds were further identified with HPLC/ESI-MS, (1)H NMR, and( 13)C NMR, among which 4-(3,4-dihydroxy-phenyl)-but-3-en-2-one, acacetin-7-diglucuronide, and luteolin-7-diglucuronide were the first to be identified from this plant. The results demonstrated that multi-channel parallel preparative HPLC/UV/MS is an efficient method for isolation and purification of compounds from natural products.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Wang
authors.forename: Xingya
authors.initials: X
authors.lastname: Xue
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Xiao
authors.forename: Feifang
authors.initials: F
authors.lastname: Zhang
authors.forename: Qing
authors.initials: Q
authors.lastname: Xu
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods; D005419:Flavonoids / Q000737:chemistry; D006027:Glycosides / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D008516:Medicine, Chinese Traditional; D008956:Models, Chemical; D004364:Pharmaceutical Preparations; D019800:Phenol / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis* / Q000302:isolation & purification; D031363:Scutellaria / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D014466:Ultraviolet Rays
chemical_list: D005419:Flavonoids; D006027:Glycosides; D004364:Pharmaceutical Preparations; D010936:Plant Extracts; D019800:Phenol