title: The use of amnion-derived cellular cytokine solution to improve healing in acute and chronic wound models.
abstract: OBJECTIVE
  Growth factors demonstrate mixed results improving wound healing. Amnion-derived multipotent cells release physiologic levels of growth factors and tissue inhibitors of metalloproteinases. This solution was tested in models of acute and chronic wound healing.
  
  
  METHODS
  Acute model: Sprague-Dawley rats underwent laparotomy incisions. The midline fascia was primed with phosphate-buffered saline, unconditioned media, or amnion-derived cellular cytokine suspension prior to incision. Breaking strength of laparotomy wounds was tested with an Instron tensiometer. Incisional hernia formation was measured after 28 days. Chronic model: Chronic, infected granulating wounds were produced in rats by excising full thickness burn eschars inoculated with Escherica coli. Granulating wounds were treated with unconditioned media or amnion-derived cellular cytokine suspension. Treatments were applied either on day 0 and day 7 or day 0 and then every other day. Wounds were traced every 72 hours and biopsied for quantitative bacteriology.
  
  
  RESULTS
  Acute model: Priming with amnion-derived cellular cytokine suspension increased the breaking strength of laparotomy incisions in comparison with phosphate-buffered saline or unconditioned media (P < .05). Acute wound failure and incisional hernia formation was 100% in the phosphate-buffered saline and unconditioned media groups and 18% in the amnion-derived cellular cytokine suspension-treated group (P <.05). Chronic model: The rate of wound closure was accelerated in amnion-derived cellular cytokine suspension-treated chronic wounds (P < .05). Multidosing improved the effect.
  
  
  CONCLUSIONS
  A physiologic solution of cytokines and tissue inhibitors of metalloproteinases improves healing in models of acute and chronic wounds. Such a cocktail can be produced from amnion-derived multipotent progenitor cells.
authors.affiliation: University of Michigan, Ann Arbor, MI, USA. mfranz@med.umich.edu
authors.forename: Michael G
authors.initials: MG
authors.lastname: Franz
authors.forename: Wyatt G
authors.initials: WG
authors.lastname: Payne
authors.forename: Liyu
authors.initials: L
authors.lastname: Xing
authors.forename: D K
authors.initials: DK
authors.lastname: Naidu
authors.forename: R E
authors.initials: RE
authors.lastname: Salas
authors.forename: Vivienne S
authors.initials: VS
authors.lastname: Marshall
authors.forename: C J
authors.initials: CJ
authors.lastname: Trumpower
authors.forename: Charlotte A
authors.initials: CA
authors.lastname: Smith
authors.forename: David L
authors.initials: DL
authors.lastname: Steed
authors.forename: M C
authors.initials: MC
authors.lastname: Robson