[Serologic diagnostics of Legionella infection].
Technical approaches to construction of preparations for serologic diagnostics of Legionella infection were presented in the article; antigenic- and immunoglobulin-based diagnostic kits with known characteristics were developed. Immunogenic properties of protein and lypopolysaccharide antigens, which have diagnostic value, were studied; similarity of protein antigens from 7 serogroups of L. pneumophila was demonstrated. Soluble antigen with known composition was obtained and used for the development of antigen-based polymeric kit for diagnostics of Legionella infection. On the basis of hyperimmune sera, immunoglobulin-based polymeric diagnostic kit and array of coagglutinating diagnostic kits for the mentioned 7 serogroups were developed. Antigen-based polymeric diagnostic kit was recommended for licensure.
Karbyshev
G L
GL
Terent'ev
A N
AN
Verkina
L M
LM
Simonova
I R
IR
Kochetkova
A P
AP
Narkevich
A N
AN
Lysova
L K
LK
Larionova
L V
LV
D000371:Agglutination; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology*; D000918:Antibody Specificity; D000942:Antigens, Bacterial / Q000032:analysis / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology; D001431:Bacteriological Techniques / Q000379:methods*; D006801:Humans; D007106:Immune Sera; D007124:Immunoenzyme Techniques; D007136:Immunoglobulins; D007875:Legionella / Q000276:immunology*; D007876:Legionellosis / Q000175:diagnosis* / Q000382:microbiology / Q000652:urine; D008070:Lipopolysaccharides / Q000032:analysis / Q000276:immunology; D008863:Microspheres; D011108:Polymers; D011817:Rabbits; D011933:Reagent Kits, Diagnostic / Q000382:microbiology; D012680:Sensitivity and Specificity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007106:Immune Sera; D007136:Immunoglobulins; D008070:Lipopolysaccharides; D011108:Polymers; D011933:Reagent Kits, Diagnostic