Principles of creation of protein carriers of DNA new derivatives of human epidermal growth factor for gene therapy.
We formulated a new approach to the creation of transport proteins for the delivery of foreign DNA to target cells and used it for obtaining a polypeptide PGEk. Structural and functional analysis of PGEk--DNA complexes demonstrated good prospects for the creation of a wide spectrum of targeted preparations for gene therapy. These approaches and regularities are necessary for construction of new DNA carriers selective for various cell types.
Pozmogova
G E
GE
Laboratory of Artificial Antibodygenesis, Research Institute of Physicochemical Medicine, Federal Agency for Health Care and Social Development, Russian Academy of Sciences, Moscow. pozm@mail.ru
Chuvilin
A N
AN
D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D004247:DNA / Q000378:metabolism* / Q000648:ultrastructure; D004815:Epidermal Growth Factor / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D018014:Gene Transfer Techniques; D015316:Genetic Therapy / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use; D006801:Humans; D046911:Macromolecular Substances / Q000627:therapeutic use; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000627:therapeutic use
D002352:Carrier Proteins; D046911:Macromolecular Substances; D011994:Recombinant Proteins; D004815:Epidermal Growth Factor; D004247:DNA