Peripheral blood serum from healthy donors contains antibodies against the fragment of transcribed region of ribosomal repeat.
Enzyme immunoassay showed that blood serum from healthy donors contains specific high-affinity antibodies (apparent association constant > or = 5 x 10(9) M(-1)) against a fragment of transcribed region of ribosomal DNA repeat of human serum, which are present in a free form or are bound to extracellular DNA. Preheating of the serum at 55 degrees C and high ionic strength (1.5 M NaCl) had no effect on the interaction of antibodies with this fragment. Competitive binding assay showed that these antibodies recognize DNA epitopes, which differ from the epitopes recognized by most anti-DNA antibodies in systemic lupus erythematosus.
Veiko
N N
NN
Medical and Genetic Research Center, Russian Academy of Medical Sciences. ribgene@rambler.ru
Kostyuk
S V
SV
Ermakov
A V
AV
Kalashnikova
E A
EA
Kupavtseva
O A
OA
Ryazantseva
T A
TA
Speranskii
A I
AI
D000328:Adult; D000818:Animals; D000974:Antibodies, Antinuclear / Q000097:blood*; D004275:DNA, Ribosomal / Q000276:immunology*; D005260:Female; D006801:Humans; D044967:Serum / Q000276:immunology*
D000974:Antibodies, Antinuclear; D004275:DNA, Ribosomal