title: Ductal injection of preservation solution increases islet yields in islet isolation and improves islet graft function.
abstract: For islet transplantation, it is important to obtain an available islet mass adequate for diabetes reversal from a single donor pancreas. A recent report demonstrated that the use of M-Kyoto solution instead of UW solution improved islet yields in the two-layer method for pancreas preservation. The present study investigated whether the ductal injection of a large volume of preservation solution (UW and M-Kyoto solution) before pancreas storage improves islet yields. Islet yield both before and after purification was significantly higher in the ductal injection (+) group compared with the ductal injection (-) group. TUNEL-positive cells in the ductal injection (+) group were significantly decreased in comparison to the ductal injection (-) group. The ductal injection of preservation solution increased the ATP level in the pancreas tissue and reduced trypsin activity during the digestion step. Annexin V and PI assays showed that the ductal injection prevents islet apoptosis. In a transplant model, the ductal injection improved islet graft function. These findings suggest that the ductal injection of preservation solution, especially the M-Kyoto solution, leads to improved outcomes for pancreatic islet transplantation. Based on these data, this technique is now used for clinical islet transplantation from non-heart-beating donor pancreata or living donor pancreas.
authors.affiliation: Transplantation Unit, Kyoto University Hospital, Kyoto 606-8507, Japan. noguchih@med.nagoya-u.ac.jp
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Noguchi
authors.forename: Michiko
authors.initials: M
authors.lastname: Ueda
authors.forename: Shuji
authors.initials: S
authors.lastname: Hayashi
authors.forename: Naoya
authors.initials: N
authors.lastname: Kobayashi
authors.forename: Teru
authors.initials: T
authors.lastname: Okitsu
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Iwanaga
authors.forename: Hideo
authors.initials: H
authors.lastname: Nagata
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakai
authors.forename: Shinichi
authors.initials: S
authors.lastname: Matsumoto
mesh_terms: D000241:Adenosine / Q000008:administration & dosage; D000493:Allopurinol / Q000008:administration & dosage; D000818:Animals; D002469:Cell Separation / Q000379:methods; D004333:Drug Administration Routes; D005978:Glutathione / Q000008:administration & dosage; D006085:Graft Survival; D007328:Insulin / Q000008:administration & dosage; D007515:Islets of Langerhans / Q000033:anatomy & histology; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000008:administration & dosage*; D011887:Raffinose / Q000008:administration & dosage; D013552:Swine; D014183:Transplantation, Heterologous
chemical_list: D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose