A minimal fragment of MUC1 mediates growth of cancer cells.
The MUC1 protein is aberrantly expressed on many solid tumor cancers. In contrast to its apical clustering on healthy epithelial cells, it is uniformly distributed over cancer cells. However, a mechanistic link between aberrant expression and cancer has remained elusive. Herein, we report that a membrane-bound MUC1 cleavage product, that we call MUC1*, is the predominant form of the protein on cultured cancer cells and on cancerous tissues. Further, we demonstrate that transfection of a minimal fragment of MUC1, MUC1*(1110), containing a mere forty-five (45) amino acids of the extracellular domain, is sufficient to confer the oncogenic activities that were previously attributed to the full-length protein. By comparison of molecular weight and function, it appears that MUC1* and MUC1*(1110) are approximately equivalent. Evidence is presented that strongly supports a mechanism whereby dimerization of the extracellular domain of MUC1* activates the MAP kinase signaling cascade and stimulates cell growth. These findings suggest methods to manipulate this growth mechanism for therapeutic interventions in cancer treatments.
Mahanta
Sanjeev
S
Minerva Biotechnologies, Waltham, Massachusetts, United States of America.
Fessler
Shawn P
SP
Park
Jaehong
J
Bamdad
Cynthia
C
D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D002462:Cell Membrane / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D006801:Humans; D008024:Ligands; D008969:Molecular Sequence Data; D018396:Mucin-1 / Q000737:chemistry / Q000378:metabolism*; D050505:Mutant Proteins / Q000378:metabolism; D009369:Neoplasms / Q000473:pathology*; D010446:Peptide Fragments / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D051381:Rats; D017978:Receptors, Growth Factor / Q000378:metabolism
D000906:Antibodies; D008024:Ligands; D018396:Mucin-1; D050505:Mutant Proteins; D010446:Peptide Fragments; D017978:Receptors, Growth Factor