Evaluation of a multicolor, single-tube technique to enumerate lymphocyte subpopulations.
To evaluate the fully automated FACSCanto software, we compared lymphocyte subpopulation counts obtained using three-color FACSCalibur-CELLQuest and six-color FACSCanto-FACSCanto software techniques. High correlations were observed between data obtained with these techniques. Our study indicated that FACSCanto clinical software is accurate and sensitive in single-platform lymphocyte immunophenotyping.
Colombo
F
F
Cellular Therapy and Cryobiology, Regenerative Medicine Department, Centre of Transfusion Medicine, IRCCS Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy. labcitofl@policlinico.mi.it
Cattaneo
A
A
Lopa
R
R
Portararo
P
P
Rebulla
P
P
Porretti
L
L
D015703:Antigens, CD / Q000276:immunology; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D016130:Immunophenotyping / Q000379:methods*; D016131:Lymphocyte Subsets / Q000276:immunology*; D012680:Sensitivity and Specificity; D012984:Software
D015703:Antigens, CD