title: Myocardial beta-adrenergic receptor characteristics in T(3)-induced ascites and in broiler lines differing in ascites susceptibility.
abstract: The present study was designed to investigate the myocardial beta-adrenergic receptor characteristics in T3-induced ascites in two genetic lines of broilers, in order to look for possible involvement of myocardial beta-adrenergic receptors in the development of broiler ascites syndrome. Myocardial membrane fractions were prepared from 6 and 8 week old chickens fed a 1.5 parts/10(6) T(3) supplemented diet from day 1 in order to increase ascites incidence. Also, a similar assay was performed on myocardial cells of ascites sensitive and ascites resistant chickens at 5 weeks of age. The binding capacity and binding affinity of the myocardial beta-adrenergic receptor of the two genetic lines of birds did not differ significantly, but the tendency of beta-adrenergic receptor binding capacity and affinity constants in ascites-sensitive birds was slightly higher compared to ascites-resistant birds. In commercial broilers, although dietary T(3) significantly increased ascites mortality and the RV/TV ratio compared to control, it did not affect significantly beta-adrenergic receptor characteristics.
authors.affiliation: Laboratory for Physiology and Immunology of Domestic Animals, Heverlee, Belgium.
authors.forename: M H
authors.initials: MH
authors.lastname: Ladmakhi
authors.forename: N
authors.initials: N
authors.lastname: Buys
authors.forename: A
authors.initials: A
authors.lastname: Vanderpooten
authors.forename: E
authors.initials: E
authors.lastname: Decuypere