title: Rhabdomyosarcoma subtyping by immunohistochemical assessment of myogenin: tissue array study and review of the literature.
abstract: Myogenin immunostaining has been described as a useful marker of the alveolar subtype of rhabdomyosarcoma and as a tool for distinguishing it from the more common embryonal subtype. To add to the growing body of literature describing this phenomenon we analysed myogenin immunohistochemical staining in 152 tumors using a rhabdomyosarcoma tissue array. Results were analysed blinded to histological type by two independent investigators. Samples were excluded if any samples failed to stain with desmin and/or myogenin. Mean percentage of myogenin positive cells was significantly greater for ARMS (n = 31; mean percentage positivity 59% (95% confidence intervals +/- 7%) than ERMS (n = 41, mean percentage positivity 16%, 95% confidence intervals +/- 4; P < 0.0001). This data is consistent with previously published studies identifying strong nuclear myogenin staining in a high proportion of cells as a marker of alveolar histology.
authors.affiliation: Unit of Molecular Haematology and Cancer Biology, Institute of Child Health, London, UK.
authors.forename: D A
authors.initials: DA
authors.lastname: Morgenstern
authors.forename: H
authors.initials: H
authors.lastname: Rees
authors.forename: N J
authors.initials: NJ
authors.lastname: Sebire
authors.forename: J
authors.initials: J
authors.lastname: Shipley
authors.forename: John
authors.initials: J
authors.lastname: Anderson
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D003937:Diagnosis, Differential; D006801:Humans; D018008:Myogenin / Q000378:metabolism*; D011379:Prognosis; D018232:Rhabdomyosarcoma, Alveolar / Q000145:classification / Q000175:diagnosis / Q000378:metabolism*; D018233:Rhabdomyosarcoma, Embryonal / Q000145:classification / Q000175:diagnosis / Q000378:metabolism*; D012680:Sensitivity and Specificity; D046888:Tissue Array Analysis
chemical_list: D014408:Biomarkers, Tumor; D018008:Myogenin