title: The -30G>A polymorphism of the glucokinase gene promoter is associated with obesity in a population from southern Spain.
abstract: The aim of this study was to test the hypothesis of an association between the -30G>A polymorphism of the promoter of the glucokinase gene and the prevalence and incidence of obesity. We studied the -30G>A polymorphism of the glucokinase gene promoter in 981 persons, of whom 866 were seen again 6 years later. All the persons underwent an oral glucose-tolerance test and the BMI (weight/height(2)) was recorded. The -30G>A polymorphism of the glucokinase gene promoter was studied using RFLP-PCR. At the initial study, the probability of having a BMI > or =25 in carriers of the A allele was significantly lower than expected by chance (odds ratio (OR) = 0.63; 95% confidence interval (CI) = 0.456-0.885). In those persons with a BMI > or =30 at the first study, the probability at 6 years of losing weight (reaching a BMI < 30) was greater in carriers of the A allele (OR = 0.22; 95% CI = 0.087-0.576). The increase in weight over these 6 years, taken as a continuous variable, was significantly less only in those persons who were originally obese (P = 0.018). In conclusion, in a population from southern Spain, carriers of the A allele of the -30G>A polymorphism in the promoter of the glucokinase gene had a lower risk for obesity and the likelihood of losing weight was greater in those obese persons who had the A allele (GA or AA).
authors.affiliation: Servicio de Endocrinología y Nutrición, Hospital Regional Universitario Carlos Haya (Hospital Civil)-Fundación IMABIS, Málaga, Spain. juanm.gomez.exts@juntadeandalucia.es
authors.forename: Juan M
authors.initials: JM
authors.lastname: Gómez-Zumaquero
authors.forename: G
authors.initials: G
authors.lastname: Rojo-Martínez
authors.forename: E
authors.initials: E
authors.lastname: García-Escobar
authors.forename: Gracia M
authors.initials: GM
authors.lastname: Martín-Nuñez
authors.forename: J
authors.initials: J
authors.lastname: Haro
authors.forename: I
authors.initials: I
authors.lastname: Esteva
authors.forename: M
authors.initials: M
authors.lastname: Ruiz de Adana
authors.forename: Antonio L
authors.initials: AL
authors.lastname: Cuesta
authors.forename: G
authors.initials: G
authors.lastname: Olveira
authors.forename: S
authors.initials: S
authors.lastname: Morcillo
authors.forename: F
authors.initials: F
authors.lastname: Soriguer
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000483:Alleles; D015992:Body Mass Index; D005260:Female; D020022:Genetic Predisposition to Disease; D005941:Glucokinase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000208:ethnology / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013030:Spain
chemical_list: D005941:Glucokinase