title: Saccharomyces cerevisiae Yta7 regulates histone gene expression.
abstract: The Saccharomyces cerevisiae Yta7 protein is a component of a nucleosome bound protein complex that maintains distinct transcriptional zones of chromatin. We previously found that one protein copurifying with Yta7 is the yFACT member Spt16. Epistasis analyses revealed a link between Yta7, Spt16, and other previously identified members of the histone regulatory pathway. In concurrence, Yta7 was found to regulate histone gene transcription in a cell-cycle-dependent manner. Association at the histone gene loci appeared to occur through binding of the bromodomain-like region of Yta7 with the N-terminal tail of histone H3. Our work suggests a mechanism in which Yta7 is localized to chromatin to establish regions of transcriptional silencing, and that one facet of this cellular mechanism is to modulate transcription of histone genes.
authors.affiliation: Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
authors.forename: Angeline
authors.initials: A
authors.lastname: Gradolatto
authors.forename: Richard S
authors.initials: RS
authors.lastname: Rogers
authors.forename: Heather
authors.initials: H
authors.lastname: Lavender
authors.forename: Sean D
authors.initials: SD
authors.lastname: Taverna
authors.forename: C David
authors.initials: CD
authors.lastname: Allis
authors.forename: John D
authors.initials: JD
authors.lastname: Aitchison
authors.forename: Alan J
authors.initials: AJ
authors.lastname: Tackett
mesh_terms: D047369:Chromatin Immunoprecipitation; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D017931:DNA Primers / Q000235:genetics; D004843:Epistasis, Genetic; D005434:Flow Cytometry; D015966:Gene Expression Regulation, Fungal / Q000235:genetics*; D006657:Histones / Q000378:metabolism*; D013058:Mass Spectrometry; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D017931:DNA Primers; D006657:Histones; D029701:Saccharomyces cerevisiae Proteins; C508095:YTA7 protein, S cerevisiae