[Immunolocalization of Secretion complex in Streptococcus mutans].
OBJECTIVE
To investigate the distribution pattern of the Secretion complex in Streptococcus mutans by means of the subcellular localization of SecA and SecY.


METHODS
The specificity of anti-SecA antibody and the anti-SecY antibody were examined by Western blot. An indirect postembedding immunogold method was used to determine the subcellular localization of the SecA and SecY in the cytoplasmic membrane of the Streptococcus mutans GS-5.


RESULTS
Immunoblotting results showed that the anti-SecA antibody and the anti-SecY antibody specifically recognized a single band of about 95 000 and 47 800 respectively. Immunogold electron microscopy revealed a single intense focus of gold particles at a discrete location on the cytoplasmic membrane of the Streptococcus mutans GS-5.


CONCLUSIONS
SecA and SecY clustered to an asymmetric microdomain, which suggests that Sec complex present a uni-site on the cytoplasmic membrane of Streptococcus mutans.
Hu
Ping
P
Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China.
Bian
Zhuan
Z
Fan
Ming-wen
MW
D000251:Adenosine Triphosphatases / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D007150:Immunohistochemistry; D026901:Membrane Transport Proteins / Q000378:metabolism*; D000069816:SEC Translocation Channels; D000081416:SecA Proteins; D013295:Streptococcus mutans / Q000378:metabolism*
D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; D000069816:SEC Translocation Channels; D000251:Adenosine Triphosphatases; D000081416:SecA Proteins