[A study of the nucleotide sequence variability of rha locus genes of Yersinia pestis main and non-main subspecies].
A study of the structural and regulatory genes, determining rhamnose fermentation, that are located in the rha locus of the chromosome of Yersinia pestis main and non-main subspecies and of Yersinia pseudotuberculosis of serogroups I-III was performed. The nucleotide sequence of Y. pestis main subspecies differs substantially from those of non-main subspecies and Y. pseudotuberculosis by the presence of a nucleotide substitution in 671 bp location of rhaS gene resulting presumably in the Y. pestis non-main subsp inability to utilize rhamnose. This results in incapability of Y. pestis non-main subspecies to utilize rhamnose. Other nucleotide substitutions found in Y. pestis non-main subspecies strains influence only upon expression efficiency of this diagnostic criterion.
Kukleva
L M
LM
Eroshenko
G A
GA
Kuklev
V E
VE
Shavina
N Iu
NIu
Krasnov
Ia M
IaM
Guseva
N P
NP
Kutyrev
V V
VV
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence / Q000235:genetics*; D005285:Fermentation / Q000235:genetics; D005798:Genes, Bacterial; D005809:Genes, Regulator; D014644:Genetic Variation; D006801:Humans; D010930:Plague / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012210:Rhamnose / Q000235:genetics / Q000378:metabolism; D012426:Russia; D015010:Yersinia pestis / Q000235:genetics*; D015011:Yersinia pseudotuberculosis / Q000235:genetics; D015012:Yersinia pseudotuberculosis Infections / Q000382:microbiology
D001426:Bacterial Proteins; D012210:Rhamnose