Glycomic analysis of alpha-fetoprotein L3 in hepatoma cell lines and hepatocellular carcinoma patients.
The N-glycan structures of the Lens culinaris agglutinin (LCA)-reactive fraction of alpha-fetoprotein (AFP-L3), a tumor marker of hepatocellular carcinomas (HCC), were analyzed in relationship to glycosyltransferases and LCA-affinity electrophoresis. Using HPLC and MALDI-TOF MS, we determined the N-glycan structures of AFP from HCC cell lines, and demonstrated they were affected by N-acetylglucosaminyltransferase III and fucosyltransferase VIII, but not by N-acetylglucosaminyltransferase V. Moreover, we identified the N-glycan structures of AFP in HCC patients.
Nakagawa
Takatoshi
T
Department of Glycotherapeutics, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Osaka 565-0871, Japan.
Miyoshi
Eiji
E
Yakushijin
Takayuki
T
Hiramatsu
Naoki
N
Igura
Takumi
T
Hayashi
Norio
N
Taniguchi
Naoyuki
N
Kondo
Akihiro
A
D014408:Biomarkers, Tumor / Q000302:isolation & purification / Q000378:metabolism; D002240:Carbohydrate Sequence; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D004587:Electrophoresis, Agar Gel; D005647:Fucosyltransferases / Q000235:genetics / Q000378:metabolism; D006023:Glycoproteins / Q000302:isolation & purification / Q000378:metabolism*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D008969:Molecular Sequence Data; D017351:N-Acetylglucosaminyltransferases / Q000235:genetics / Q000378:metabolism; D025942:Oligosaccharides, Branched-Chain / Q000302:isolation & purification / Q000378:metabolism*; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / Q000235:genetics / Q000378:metabolism; D037121:Plant Lectins / Q000737:chemistry; D034741:RNA, Small Interfering / Q000235:genetics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014162:Transfection; D000509:alpha-Fetoproteins / Q000302:isolation & purification / Q000378:metabolism*
C097353:AFP protein, human; D014408:Biomarkers, Tumor; D006023:Glycoproteins; D025942:Oligosaccharides, Branched-Chain; D037121:Plant Lectins; D034741:RNA, Small Interfering; D000509:alpha-Fetoproteins; C033619:lentil lectin; D016695:Glycosyltransferases; D005647:Fucosyltransferases; D017351:N-Acetylglucosaminyltransferases; C054599:beta-1,4-mannosyl-glycoprotein beta-1,4-N-acetylglucosaminyltransferase; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase; C020559:Glycoprotein 6-alpha-L-fucosyltransferase; D043524:Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase