Analysis of vaginal lactobacilli from healthy and infected Brazilian women.
Culture-dependent PCR-amplified rRNA gene restriction analysis and culture-independent (PCR-denaturing gradient gel electrophoresis) methodologies were used to examine vaginal lactobacilli from Brazilian women who were healthy or had been diagnosed with vulvovaginal candidiasis (VVC) or bacterial vaginosis. Only Lactobacillus crispatus was detected accordingly by both methods, and H(2)O(2)-producing lactobacilli were not associated with protection against VVC.
Martinez
Rafael C R
RC
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil.
Franceschini
Sílvio A
SA
Patta
Maristela C
MC
Quintana
Silvana M
SM
Nunes
Alvaro C
AC
Moreira
João L S
JL
Anukam
Kingsley C
KC
Reid
Gregor
G
De Martinis
Elaine C P
EC
D000704:Analysis of Variance; D001938:Brazil / Q000453:epidemiology; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology; D016009:Chi-Square Distribution; D004269:DNA, Bacterial / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D016133:Polymerase Chain Reaction; D014621:Vagina / Q000382:microbiology*; D019522:Vaginal Discharge / Q000382:microbiology*; D016585:Vaginosis, Bacterial / Q000453:epidemiology / Q000382:microbiology*
D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D006861:Hydrogen Peroxide