Immunocytochemical localization of monoamine oxidase type B in rat liver.
We used an immunohistochemical method to examine the localization of monoamine oxidase type B (MAOB) in rat liver. At the light microscopic level, MAOB was highly expressed in rat liver. It was intense around portal area, and weak around central area. All the hepatocytes examined had MAOB immunoreactivity. For the first time, using a double-labeling immunofluorescence histochemical method for laser microscopy, we report that no MAOB is found in endothelial cells, hepatic stellate cells, or Kupffer's cells. When examined under transmission electron microscopy, MAOB was localized to the mitochondrial outer membrane of hepatocytes. No apparent localization of MAOB was found in the rough endoplasmic reticulum, the crystal membrane of mitochondria, the nuclear envelope, or the plasma membrane.
Huang
Y H
YH
Department of Gastroenterology, The First Affiliated Hospital, China Medical University, Shenyang 110001, China.
Jiang
Min
M
Fu
B Y
BY
D000818:Animals; D005455:Fluorescent Antibody Technique; D022781:Hepatocytes / Q000166:cytology / Q000201:enzymology* / Q000648:ultrastructure; D007124:Immunoenzyme Techniques; D008099:Liver / Q000201:enzymology* / Q000648:ultrastructure; D008297:Male; D046529:Microscopy, Electron, Transmission; D008930:Mitochondria, Liver / Q000201:enzymology* / Q000648:ultrastructure; D051336:Mitochondrial Membranes / Q000201:enzymology* / Q000648:ultrastructure; D008995:Monoamine Oxidase / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D008995:Monoamine Oxidase