Direct printing of trichlorosilanes on glass for selective protein adsorption and cell growth.
Here we describe new methodology that allows for direct microcontact printing of octadecyltrichlorosilane onto glass coverslips followed by backfilling with an ethylene glycol terminated trichloroalkane silane; this produces patterns with regions that promote and prevent protein adsorption and allow for control of cell growth.
Yanker
Dawn M
DM
Department of Chemistry, Washington University in St. Louis, One Brookings Drive, St. Louis, MO 63130, USA.
Maurer
Joshua A
JA
D000327:Adsorption; D000818:Animals; D016466:CHO Cells; D049109:Cell Proliferation; D006224:Cricetinae; D003412:Cricetulus; D019855:Ethylene Glycol / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D005455:Fluorescent Antibody Technique; D005898:Glass / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D010316:Particle Size; D012680:Sensitivity and Specificity; D012821:Silanes / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties
D005353:Fibronectins; D007074:Immunoglobulin G; D012821:Silanes; C061189:octadecyltrichlorosilane; D019855:Ethylene Glycol; D016650:Fluorescein-5-isothiocyanate