title: Impact of pulsed electric fields on Corynebacterium glutamicum cell membrane permeabilization.
abstract: The permeability barrier of the microbial cell envelope for substrates and products often causes very low reaction rates of whole cells. Therefore, it is of interest to develop an effective method to reduce this permeability barrier in order to increase product yields. Utilisation of pulse electric fields may improve amino acid release from Corynebacterium glutamicum by up to several orders of magnitude. In particular pulsed electric fields may change the cell/membrane's dielectric properties and induce the release of intracellular metabolites. In this study the parameters for successful electropermeabilization were determined and the viabilities of treated cells were examined. We also found that pulse treated cells not only maintained their viabilities but also their ability to reproduce, post-pulse treatment. Since electropermeabilized cells could maintain both their viabilities and ability to reproduce, we believe that this preliminary data may contribute to the optimization of fermentative production of amino acids and bioprocess enhancement through electropermeabilization and may be beneficial to industrial bioprocesses.
authors.affiliation: Chemical Engineering, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh, EH14 4AS, UK. tt306@cam.ac.uk
authors.forename: Theodora
authors.initials: T
authors.lastname: Tryfona
authors.forename: Mark T
authors.initials: MT
authors.lastname: Bustard
mesh_terms: D002462:Cell Membrane; D002463:Cell Membrane Permeability; D048230:Corynebacterium glutamicum / Q000166:cytology; D018274:Electroporation / Q000379:methods*; D015203:Reproducibility of Results