title: Influence of fibrin glue on proliferation and differentiation of human bone marrow stromal cells seeded on a biologic 3-dimensional matrix.
abstract: For tissue engineering of bone, a carrier matrix and efficient cell seeding are desirable. This study analysed the effect of fibrin glue on bone marrow stromal cells (BMSC) adhesion, proliferation (MTS-Test), differentiation (alkaline phosphatase (AP), osteocalcin (OC), ELISA) and compared the results with cells seeded within culture media on a decellularized, xenogenic bone matrix. There was no significant difference regarding cell adhesion. Proliferation after one week was significantly increased without fibrin glue. AP was increased in both groups when compared with porous scaffolds without cells. OC secretion was increased under both seeding conditions. Microscopic investigation of the cells with fibrin-glue showed less cell-cell contacts. This study reveals that cell seeding with medium demonstrates similar adherence rates compared with fibrin glue. Fibrin glue significantly decreases cell proliferation. Cell differentiation with respect to ALP and OC is not affected. Further studies are required to assess the long term and in vivo effects of both methods with respect to BMSC viability and differentiation. Fibrin sealants seem not necessary to achieve cell adherence when using a porous bone matrix.
authors.affiliation: Trauma Department, Hannover Medical School (MHH), Hannover, Germany. haasper.carl@mh-hannover.de
authors.forename: Carl
authors.initials: C
authors.lastname: Haasper
authors.forename: Astrid
authors.initials: A
authors.lastname: Breitbart
authors.forename: Stefan
authors.initials: S
authors.lastname: Hankemeier
authors.forename: Michael
authors.initials: M
authors.lastname: Wehmeier
authors.forename: Eric
authors.initials: E
authors.lastname: Hesse
authors.forename: Musa
authors.initials: M
authors.lastname: Citak
authors.forename: Christian
authors.initials: C
authors.lastname: Krettek
authors.forename: Johannes
authors.initials: J
authors.lastname: Zeichen
authors.forename: Michael
authors.initials: M
authors.lastname: Jagodzinski
mesh_terms: D000328:Adult; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects*; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation; D049109:Cell Proliferation; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006801:Humans; D008875:Middle Aged; D017154:Stromal Cells / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods
chemical_list: D015718:Fibrin Tissue Adhesive