title: Lectin-based electrophoretic analysis of the expression of the 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 fragment in sera of patients with five different malignancies.
abstract: A 35 kDa glycoprotein whose abundance was previously demonstrated to be enhanced in sera of patients with endometrial adenocarcinoma (n = 12), was isolated from pooled sera of three of the cancer patients using champedak galactose-binding lectin affinity chromatography in the present study. Subjecting it to 2-DE and MS/MS, the glycoprotein was identified as the O-glycosylated fragment of inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4). When compared to control sera (n = 17), expression of the 35 kDa ITIH4 cleavage fragment was demonstrated to be significantly enhanced in sera of patients with breast carcinoma (n = 10), epithelial ovarian carcinoma (n = 10), and germ cell ovarian carcinoma (n = 10) but not in patients with nasopharyngeal carcinoma (n = 13) and osteosarcoma (n = 7). The lectin-based electrophoretic bioanalytical method adopted in the present study may be used to assess the physiological relevance of ITIH4 fragmentation and its correlation with different malignancies, their stages and progression.
authors.affiliation: Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Emida
authors.initials: E
authors.lastname: Mohamed
authors.forename: Puteri Shafinaz
authors.initials: PS
authors.lastname: Abdul-Rahman
authors.forename: Saeid Reza
authors.initials: SR
authors.lastname: Doustjalali
authors.forename: Yeng
authors.initials: Y
authors.lastname: Chen
authors.forename: Boon-Kiong
authors.initials: BK
authors.lastname: Lim
authors.forename: Siti Zawiah
authors.initials: SZ
authors.lastname: Omar
authors.forename: Anita Zarina
authors.initials: AZ
authors.lastname: Bustam
authors.forename: Vivek Ajit
authors.initials: VA
authors.lastname: Singh
authors.forename: Nur Aishah
authors.initials: NA
authors.lastname: Mohd-Taib
authors.forename: Cheng-Har
authors.initials: CH
authors.lastname: Yip
authors.forename: Onn Haji
authors.initials: OH
authors.lastname: Hashim
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000510:Alpha-Globulins / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000097:blood; D001859:Bone Neoplasms / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D037161:Galectins; D006031:Glycosylation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism; D012516:Osteosarcoma / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism; D037121:Plant Lectins; D053719:Tandem Mass Spectrometry
chemical_list: D000510:Alpha-Globulins; D014408:Biomarkers, Tumor; D037161:Galectins; D037121:Plant Lectins; C011877:inter-alpha-inhibitor