title: Influence of matrix-suspended demineralized bone on osseous repair using a critical-sized defect in the rat (Rattus norvegicus) calvarium.
abstract: Demineralized freeze-dried bone (DFDB) in matrix form must be rehydrated with a carrier medium which allows for easy manipulation during periodontal surgery. The purpose of this study was to evaluate how human DFDB suspended in a polyol matrix affects new bone formation in the rat calvarium critical-sized defect (CSD) model. Fifty-five adult male Harlan Sprague-Dawley rats were assigned to 1 of 5 treatment groups: polyol, 100% DFDB, 47% DFDB/polyol, 47% DFDB, or an unfilled control. They were then placed into 8-m calvarial CSDs. The bone donor source company for the DFDB and DFDB/polyol groups was the same. Calvaria were harvested 10 weeks after surgery and evaluated histomorphometrically. The diameter of bone particles from the 3 groups containing DFDB was measured by scanning electron microscopy. There was no statistically significant difference in the percentage of bone fill between any of the groups, although the 100% DFDB group exhibited the most bone fill. The 47% DFDB/polyol and 47% DFDB groups had similar amounts of bone formation. The average size of the demineralized bone particles from the 100% DFDB group was significantly smaller than that of the other 2 groups containing DFDB. Adding a polyol to DFDB produced similar osseous regeneration in the rat calvarium defect model vs DFDB alone. Yet from a clinical standpoint, the polyol enhanced graft handling and stability. Graft particle size may have an effect on bone fill.
authors.affiliation: U.S. Army Dental Corps, Fort Bliss, Texas, USA. bryan.kalish@us.army.mil
authors.forename: Bryan P
authors.initials: BP
authors.lastname: Kalish
authors.forename: George S
authors.initials: GS
authors.lastname: Schuster
authors.forename: Mark E
authors.initials: ME
authors.lastname: Peacock
authors.forename: Michael F
authors.initials: MF
authors.lastname: Cuenin
authors.forename: Gary D
authors.initials: GD
authors.lastname: Swiec
authors.forename: Brad J
authors.initials: BJ
authors.lastname: Potter
authors.forename: Thomas B
authors.initials: TB
authors.lastname: Buxton
authors.forename: James C
authors.initials: JC
authors.lastname: McPherson
mesh_terms: D000818:Animals; D001857:Bone Matrix / Q000637:transplantation*; D001861:Bone Regeneration / Q000187:drug effects*; D048091:Guided Tissue Regeneration / Q000379:methods; D006801:Humans; D008297:Male; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull / Q000601:surgery; D018709:Statistics, Nonparametric; D014945:Wound Healing / Q000187:drug effects
chemical_list: D008567:Membranes, Artificial; D011108:Polymers; C024617:polyol