Label-free detection of sequence-specific DNA with multiwalled carbon nanotubes and their light scattering signals.
A detection method for DNA sequence-specificity in a homogeneous medium is presented with multiwalled carbon nanotubes (MWCNTs) as optical probes on the basis of the measurements of light scattering signals. ssDNA can prevent MWCNTs from coagulation in electrolyte solution while dsDNA cannot, displaying different light scattering signals. With the light scattering signals, target DNA in the range of 8.6-86.4 nM could be detected and one base pair mismatch could be discriminated. The sequence specificity for the present method has been identified with PCR products.
Zhang
Li
L
College of Chemistry and Chemical Engineering, MOE Key Laboratory of Luminescence and Real-Time Analysis, CQKL-LRTA, Southwest University, Chongqing, China.
Huang
Cheng Zhi
CZ
Li
Yuan Fang
YF
Xiao
Sai Jin
SJ
Xie
Jian Ping
JP
D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D008027:Light; D037742:Nanotubes, Carbon; D012542:Scattering, Radiation
D017931:DNA Primers; D037742:Nanotubes, Carbon; D004247:DNA