Electrotransformation of Bacillus mojavensis with fluorescent protein markers.
Gram-positive endophytic bacteria are difficult to transform. To study the interactions between Bacillus mojavensis and maize, a method was developed to transform the microbe by electroporation with three integration plasmids expressing green, cyan and yellow fluorescent proteins (GFP variants). GFP Transformations were verified by antibiotic selection, microscopy and amylase deficiency, based on incorporation of the plasmids through recombination with the amylase gene. Phenotypic expressions of endophytism and fungal antagonisms of the transformants were the same as wild type stains. This represents the first successful transformation of this endophytic species with GFP markers.
Olubajo
Babatunde
B
USDA, ARS, Toxicology and Mycotoxin Research Unit, Russell Research Center, P.O. Box 5677, Athens GA 30605, United States.
Bacon
Charles W
CW
D000681:Amylases / Q000235:genetics; D001407:Bacillus / Q000235:genetics* / Q000502:physiology; D001426:Bacterial Proteins / Q000235:genetics; D018274:Electroporation / Q000379:methods*; D017353:Gene Deletion; D017930:Genes, Reporter; D008164:Luminescent Proteins / Q000235:genetics* / Q000378:metabolism*; D010957:Plasmids; D011995:Recombination, Genetic; D014169:Transformation, Bacterial
D001426:Bacterial Proteins; D008164:Luminescent Proteins; D000681:Amylases