title: Determination of Schistosoma japonicum circulating antigens in dilution serum by piezoelectric immunosensor and S/N enhancement.
abstract: A piezoelectric immunosensor assay was developed with immobilizing immunoglobulin G (IgG) as a probe to detect Schistosoma japonicum circulating antigens (SjCAg). Analytical strategy utilizes the polyclonal antibodies with broad-spectrum recognition to a complex target with high specificity. The immobilized antibodies were purified from immunized rabbit's sera (im-S) and infected rabbit's sera (inf-S) by S. japonicum. The detection capacities of antibodies were compared between the sera of different phenotypes and purified fractions. The sample dilution ratios were also evaluated and optimized. Additionally, the sera with a variety of infection degrees for validation could be discriminated quantitatively. The linear dose-response relationship indicates that the systematic sensitivity of this method is below 150 Hz and the lowest limit of detectable range is above 500 cercariae of S. japonicum infection for 2 weeks. The novel immunosensor technique is well potential to determine the SjCAg in serum samples for clinical diagnosis of parasitosis in early stage.
authors.affiliation: Department of Biotechnology, Ming-Chuan University, Gui-Shan, Taiwan.
authors.forename: Chien-Wei
authors.initials: CW
authors.lastname: Cheng
authors.forename: Ching-Kuo
authors.initials: CK
authors.lastname: Chen
authors.forename: Yi-Shen
authors.initials: YS
authors.lastname: Chen
authors.forename: Liang-Yu
authors.initials: LY
authors.lastname: Chen
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011791:Quartz / Q000737:chemistry; D012549:Schistosoma japonicum / Q000276:immunology*
chemical_list: D000947:Antigens, Helminth; D011791:Quartz