A regulatory B cell subset with a unique CD1dhiCD5+ phenotype controls T cell-dependent inflammatory responses.
B cells mediate multiple functions that influence immune and inflammatory responses. In this study, T cell-mediated inflammation was exaggerated in CD19-deficient (Cd19(-/-)) mice and wild-type mice depleted of CD20(+) B cells, whereas inflammation was substantially reduced in mice with hyperactive B cells as a result of CD19 overexpression (hCD19Tg). These inflammatory responses were negatively regulated by a unique CD1d(hi)CD5(+) B cell subset that was absent in Cd19(-/-) mice, represented only 1%-2% of spleen B220(+) cells in wild-type mice, but was expanded to approximately 10% of spleen B220(+) cells in hCD19Tg mice. Adoptive transfer of these CD1d(hi)CD5(+) B cells normalized inflammation in wild-type mice depleted of CD20(+) B cells and in Cd19(-/-) mice. Remarkably, IL-10 production was restricted to this CD1d(hi)CD5(+) B cell subset, with IL-10 production diminished in Cd19(-/-) mice, yet increased in hCD19Tg mice. Thereby, CD1d(hi)CD5(+) B cells represent a unique subset of potent regulatory B cells.
Yanaba
Koichi
K
Department of Immunology, Duke University Medical Center, Durham, NC 27710, USA.
Bouaziz
Jean-David
JD
Haas
Karen M
KM
Poe
Jonathan C
JC
Fujimoto
Manabu
M
Tedder
Thomas F
TF
D000818:Animals; D018949:Antigens, CD1 / Q000032:analysis / Q000276:immunology; D055610:Antigens, CD1d; D016175:B-Lymphocyte Subsets / Q000276:immunology* / Q000378:metabolism; D018956:CD5 Antigens / Q000032:analysis / Q000276:immunology; D016207:Cytokines / Q000276:immunology / Q000378:metabolism*; D016130:Immunophenotyping; D007249:Inflammation / Q000276:immunology* / Q000378:metabolism; D016753:Interleukin-10 / Q000276:immunology / Q000378:metabolism*; D008212:Lymphocyte Depletion; D051379:Mice; D008817:Mice, Mutant Strains; D008822:Mice, Transgenic; D010529:Peritoneal Cavity / Q000166:cytology; D010641:Phenotype; D013154:Spleen / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism
D018949:Antigens, CD1; D055610:Antigens, CD1d; D018956:CD5 Antigens; D016207:Cytokines; D016753:Interleukin-10