Column reversed-phase high-performance liquid chromatographic method for simultaneous determination of rabeprazole sodium and domperidone in combined tablet dosage form.
A new, simple column reversed-phase high-performance liquid chromatographic (HPLC) method for simultaneous determination of rabeprazole sodium (RAB) and domperidone (DOM) in a combined tablet dosage form has been developed and validated. Determination was performed using a Jasco HPLC system with a HiQ SiL octadecylsilane (C18) column (250 x 4.6 mm id), acetonitrile-0.1 M ammonium acetate (50 + 50, v/v) mobile phase, and paracetamol as an internal standard. The detection was performed using a UV detector set at 280 nm. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 1.0-10.0 and 0.5-5.0 microg/mL for RAB and DOM, respectively. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.
Sabnis
Shweta Sadanand
SS
All India Shri Shivagi Memorial Society's College of Pharmacy, Department of Pharmaceutical Analysis, Kennedy Rd, Pune 411001, Maharashtra, India.
Dnvandev
Dhavale Nilesh
DN
Jadhav
Vijay Yeshawantrao
VY
Gandhi
Santosh Vilashchand
SV
D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004294:Domperidone / Q000032:analysis*; D004338:Drug Combinations; D064750:Rabeprazole; D013607:Tablets
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D004338:Drug Combinations; D013607:Tablets; D064750:Rabeprazole; D004294:Domperidone