[Apoptotic mechanism of gastric carcinoma cells induced by matrine injection].
OBJECTIVE
To investigate the mechanism of gastric carcinoma cells apoptosis induced by matrine injection in vitro.


METHODS
Effects of 24, 48, 72, 96 h incubation with different concentrations (0.25-1.5 g/L) of matrine injection on proliferation of SGC-7901 cells were evaluated using 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. The cellular morphology of SGC-7901 cells was observed by transmission electron microscope (EM). Flow cytometry was used to analyze the apoptosis of SGC-7901 cells by staining with annexin V-FITC/PI. The expression of Fas/FasL was examined by flow cytometry using specific antibody. The activity of caspase-3 was measured by spectrofluorometry.


RESULTS
Matrine injection could inhibit the proliferation of SGC-7901 cells in a dose- and time-dependent manner. The typical morphological changes of apoptosis were observed after incubation with 1.0 g/L matrine injections for 48 h. The apoptosis rates of 0.5 g/L, 1.0 g/L and 1.5 g/L groups were 39.80%, 58.11% and 79.00% respectively. The apoptotic cells in matrine injection group were mainly early apoptotic cells, and those in 5-FU group were mainly late apoptotic cells and necrotic cells. Spectrofluorometry revealed FI levels of Fas and FasL were equal, which were both correlated with apoptosis rate. The activity of caspase-3 increased with the elevation of matrine concentration, and was correlated with the apoptosis rate.


CONCLUSIONS
Matrine injection can induce apoptosis of SGC-7901 cells through the up-regulation of Fas/FasL expression and activation of caspase-3.
Dai
Zhi-Jun
ZJ
Department of Oncology, The Second Affiliated Hospital, Xi'an Jiaotong University Medical College, Xi'an 710004, China. dzj0911@126.com
Gao
Jie
J
Wang
Xi-Jing
XJ
Ji
Zong-Zheng
ZZ
Wu
Wen-Ying
WY
Liu
Xiao-Xu
XX
Kang
Hua-Feng
HF
Guan
Hai-Tao
HT
Ren
Hong-Tao
HT
D000470:Alkaloids / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D045744:Cell Line, Tumor; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D011807:Quinolizines / Q000494:pharmacology*; D013274:Stomach Neoplasms; D015854:Up-Regulation; D019014:fas Receptor / Q000378:metabolism; D000093842:Matrines
D000470:Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D053222:Fas Ligand Protein; D019253:Proto-Oncogene Proteins c-bcl-2; D011807:Quinolizines; D019014:fas Receptor; D053148:Caspase 3; D000093842:Matrines