Changes of the hepatic proteome in hepatitis B-infected mouse model at early stages of fibrosis.
Liver fibrosis (LF) is the accumulation of extracellular matrix (ECM) proteins due to chronic liver injury. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to perform a comparative analysis of cytosolic and nuclear protein patterns of nontransgenic (NTg) and HBV transgenic (Tg) mice livers at early stages of fibrosis. We identified several candidate proteins, involved in a variety of pathways, which could be used as putative biomarkers for LF early detection.
Spano
Daniela
D
CEINGE Biotecnologie Avanzate, Napoli, Italy.
Cimmino
Flora
F
Capasso
Mario
M
D'Angelo
Fulvio
F
Zambrano
Nicola
N
Terracciano
Luigi
L
Iolascon
Achille
A
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D006509:Hepatitis B / Q000150:complications*; D007633:Keratins / Q000378:metabolism; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D008103:Liver Cirrhosis / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009687:Nuclear Proteins / Q000378:metabolism; D010766:Phosphorylation; D020543:Proteome / Q000378:metabolism*; D013482:Superoxide Dismutase / Q000096:biosynthesis
D015415:Biomarkers; D009687:Nuclear Proteins; D020543:Proteome; D007633:Keratins; D013482:Superoxide Dismutase