Transmission blocking immunity in the malaria non-vector mosquito Anopheles quadriannulatus species A.
Despite being phylogenetically very close to Anopheles gambiae, the major mosquito vector of human malaria in Africa, Anopheles quadriannulatus is thought to be a non-vector. Understanding the difference between vector and non-vector mosquitoes can facilitate development of novel malaria control strategies. We demonstrate that An. quadriannulatus is largely resistant to infections by the human parasite Plasmodium falciparum, as well as by the rodent parasite Plasmodium berghei. By using genetics and reverse genetics, we show that resistance is controlled by quantitative heritable traits and manifested by lysis or melanization of ookinetes in the mosquito midgut, as well as by killing of parasites at subsequent stages of their development in the mosquito. Genes encoding two leucine-rich repeat proteins, LRIM1 and LRIM2, and the thioester-containing protein, TEP1, are identified as essential in these immune reactions. Their silencing completely abolishes P. berghei melanization and dramatically increases the number of oocysts, thus transforming An. quadriannulatus into a highly permissive parasite host. We hypothesize that the mosquito immune system is an important cause of natural refractoriness to malaria and that utilization of this innate capacity of mosquitoes could lead to new methods to control transmission of the disease.
Habtewold
Tibebu
T
Immunology and Infection, Division of Cell and Molecular Biology, Faculty of Natural Sciences, Imperial College London, London, United Kingdom.
Povelones
Michael
M
Blagborough
Andrew M
AM
Christophides
George K
GK
D000818:Animals; D000852:Anopheles / Q000276:immunology* / Q000469:parasitology; D000913:Antibodies, Protozoan / Q000276:immunology; D002352:Carrier Proteins / Q000235:genetics / Q000276:immunology; D004199:Disease Vectors; D005260:Female; D020868:Gene Silencing; D006790:Host-Parasite Interactions; D007113:Immunity, Innate / Q000235:genetics / Q000276:immunology*; D019476:Insect Proteins / Q000235:genetics / Q000276:immunology; D000090263:Leucine-Rich Repeat Proteins; D008288:Malaria / Q000276:immunology / Q000469:parasitology / Q000635:transmission*; D051379:Mice; D010962:Plasmodium berghei / Q000276:immunology / Q000472:pathogenicity; D010963:Plasmodium falciparum / Q000276:immunology / Q000472:pathogenicity; D011506:Proteins / Q000235:genetics / Q000276:immunology
D000913:Antibodies, Protozoan; D002352:Carrier Proteins; D019476:Insect Proteins; D000090263:Leucine-Rich Repeat Proteins; D011506:Proteins