title: Comparative study of Cyperus rotundus essential oil by a modified GC/MS analysis method. Evaluation of its antioxidant, cytotoxic, and apoptotic effects.
abstract: Gas chromatography coupled with mass spectrometry (GC/MS), using both electron impact (EI) and chemical ionization (CI) detection modes on apolar and polar stationary phases, led to the determination of the volatile composition of the essential oil obtained from tubers of Cyperus rotundus (Cyperaceae). In this study, more than 33 compounds were identified and then compared with the results obtained in our previous work. Cyperene, alpha-cyperone, isolongifolen-5-one, rotundene, and cyperorotundene were the principal compounds comprising 62% of the oil. An in vitro cytotoxicity assay with MTT indicated that this oil was very effective against L1210 leukaemia cells line. This result correlates with significantly increased apoptotic DNA fragmentation. The oxidative effects of the essential oil were evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH), xanthine/xanthine oxidase assays, and the scavenging of superoxide radical assay generated by photo-reduction of riboflavin. The antimutagenic activity of essential oil has been examined by following the inhibition of H(2)O(2) UV photolysis which induced strand-break formation in pBS plasmid DNA scission assay. Based on all these results, it is concluded that C. rotundus essential-oil composition established by GC/MS analysis, in EI- and CI-MS modes, presents a variety of a chemical composition we were not able to detect with only GC/MS analysis in our previous work. This essential oil exhibited antioxidant, cytotoxic, and apoptotic properties.
authors.affiliation: Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculty of Pharmacy, Monastir 5000, Tunisia.
authors.forename: Soumaya
authors.initials: S
authors.lastname: Kilani
authors.forename: Jérôme
authors.initials: J
authors.lastname: Ledauphin
authors.forename: Ines
authors.initials: I
authors.lastname: Bouhlel
authors.forename: Mohamed
authors.initials: M
authors.lastname: Ben Sghaier
authors.forename: Jihed
authors.initials: J
authors.lastname: Boubaker
authors.forename: Inès
authors.initials: I
authors.lastname: Skandrani
authors.forename: Ridha
authors.initials: R
authors.lastname: Mosrati
authors.forename: Kamel
authors.initials: K
authors.lastname: Ghedira
authors.forename: Daniel
authors.initials: D
authors.lastname: Barillier
authors.forename: Leila
authors.initials: L
authors.lastname: Chekir-Ghedira
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D029786:Cyperus / Q000737:chemistry*; D005609:Free Radicals / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D051379:Mice; D015394:Molecular Structure; D009822:Oils, Volatile / Q000737:chemistry* / Q000494:pharmacology*; D010777:Photochemistry; D010936:Plant Extracts / Q000737:chemistry; D010957:Plasmids / Q000235:genetics; D014969:Xanthine Oxidase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D009822:Oils, Volatile; D010936:Plant Extracts; D006861:Hydrogen Peroxide; D014969:Xanthine Oxidase