title: High histone deacetylase 7 (HDAC7) expression is significantly associated with adenocarcinomas of the pancreas.
abstract: BACKGROUND
  Alterations in HDACs gene expression have been reported in a number of human cancers. No information is available concerning the status of HDACs in pancreatic cancer tumors. The aim of the present study was to evaluate the expression levels of members of class I (HDAC1, 2,, 3), class II (HDAC4, 5, 6, and 7), and class III (SIRT1, 2, 3, 4, 5, and 6) in a set of surgically resected pancreatic tissues.
  
  
  METHODS
  Total RNA was isolated from 11 pancreatic adenocarcinomas (PA): stage 0 (n = 1), IB (n = 1), IIB (n = 6), III (n = 1), IV (n = 2), one serous cystadenoma (SC), one intraductal papillary mucinous tumor of the pancreas (IMPN), one complicating chronic pancreatitis (CP), and normal pancreas (NP) obtained during donor liver transplantation. Moreover, six other control pancreatic were included. HDACs gene expression was conducted using quantitative real-time polymerase chain reaction (qPCR). Protein expression levels were analyzed by Western blot and their localization by immunohistochemistry analyses of cancer tissues sections.
  
  
  RESULTS
  Remarkably, 9 of the 11 PA (approximately 81%) showed significant increase of HDAC7 mRNA levels. In contrast to PA samples, message for HDAC7 was reduced in CP, SC, and IMPN specimens. The Western blot analysis showed increased expression of HDAC7 protein in 9 out of 11 PA samples, in agreement with the qPCR data. Most of the PA tissue sections examined showed intense labeling in the cytoplasm when reacted against antibodies to HDAC7.
  
  
  CONCLUSIONS
  The data showed alteration of HDACs gene expression in pancreatic cancer. Increased expression of HDAC7 discriminates PA from other pancreatic tumors.
authors.affiliation: Service de Chirurgie Digestive et Oncologique, Pôle d'Oncologie et Spécialité Médico-Chirurgicales, Assistance Pubique-Hôpitaux de Marseille, Hôpital Timone, 264 Rue Saint Pierre, Marseille 13000, France. mehdi.ouaissi@mail.ap-hm.fr
authors.forename: Mehdi
authors.initials: M
authors.lastname: Ouaïssi
authors.forename: Igor
authors.initials: I
authors.lastname: Sielezneff
authors.forename: Ricardo
authors.initials: R
authors.lastname: Silvestre
authors.forename: Bernard
authors.initials: B
authors.lastname: Sastre
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bernard
authors.forename: Joelle Simony
authors.initials: JS
authors.lastname: Lafontaine
authors.forename: Marie José
authors.initials: MJ
authors.lastname: Payan
authors.forename: Laetitia
authors.initials: L
authors.lastname: Dahan
authors.forename: Nicolas
authors.initials: N
authors.lastname: Pirrò
authors.forename: Jean François
authors.initials: JF
authors.lastname: Seitz
authors.forename: Eric
authors.initials: E
authors.lastname: Mas
authors.forename: Dominique
authors.initials: D
authors.lastname: Lombardo
authors.forename: Ali
authors.initials: A
authors.lastname: Ouaissi
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology*; D005260:Female; D015870:Gene Expression; D015971:Gene Expression Regulation, Enzymologic; D006655:Histone Deacetylases / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D008297:Male; D010190:Pancreatic Neoplasms / Q000201:enzymology*
chemical_list: D006657:Histones; C543273:HDAC7 protein, human; D006655:Histone Deacetylases