title: Experimental reproduction of severe bluetongue in sheep.
abstract: Sheep inoculated with a virulent South African strain of bluetongue (BT) virus serotype 4 developed severe clinical signs and lesions characteristic of fulminant BT, including coronitis, hemorrhage and ulceration of the mucosal lining of the oral cavity and forestomaches, hemorrhage in the wall of the pulmonary artery, and focally extensive necrosis of skeletal muscle, especially of the neck. At necropsy, up to 14 days after infection, the infected sheep exhibited striking pulmonary edema, edema of the subcutaneous tissues and fascial planes of the head and neck, and pleural and pericardial effusion of varying severity. A reliable model for experimental reproduction of fulminant BT in sheep will facilitate future studies to better characterize the pathogenesis of this disease, particularly as it regards the mechanisms responsible for the increased vascular permeability that characterizes BT and related orbiviral diseases such as African horse sickness.
authors.affiliation: Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. njmaclachlan@ucdavis.edu
authors.forename: N J
authors.initials: NJ
authors.lastname: MacLachlan
authors.forename: J E
authors.initials: JE
authors.lastname: Crafford
authors.forename: W
authors.initials: W
authors.lastname: Vernau
authors.forename: I A
authors.initials: IA
authors.lastname: Gardner
authors.forename: A
authors.initials: A
authors.lastname: Goddard
authors.forename: A J
authors.initials: AJ
authors.lastname: Guthrie
authors.forename: E H
authors.initials: EH
authors.lastname: Venter
mesh_terms: D000818:Animals; D001819:Bluetongue / Q000473:pathology / Q000635:transmission*; D001820:Bluetongue virus / Q000302:isolation & purification / Q000472:pathogenicity; D004487:Edema / Q000473:pathology; D008334:Mandible / Q000473:pathology; D012128:Respiratory Distress Syndrome / Q000209:etiology / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000473:pathology* / Q000821:virology*; D013019:South Africa; D014774:Virulence