title: Protecting effect of vitamin E supplementation on submaximal exercise-induced oxidative stress in sedentary dogs as assessed by erythrocyte membrane fluidity and paraoxonase-1 activity.
abstract: The aim of this placebo-controlled study was to investigate the effects of oral vitamin E supplementation for 10 weeks on exercise-induced oxidative damage in untrained dogs. Eight dogs were randomly assigned to a supplementation (n=4) or control (n=4) group and underwent two isolated submaximal exercise sessions, 10 weeks apart. Blood was collected during each session to measure erythrocyte membrane fluidity (EMF), paraoxonase-1 (PON1) activity, plasma malondialdehyde (MDA) and vitamin E concentrations. These biomarkers were measured in venous blood samples collected before (t(0)), just after (t, EMF only) and 1d (t+1d) and 7d (t+7d) after the dogs ran on a treadmill. Prior to vitamin E supplementation, exercise induced a significant decrease in PON1 activity, EMF, vitamin E concentration and a significant increase in MDA concentration at t+1d. After a 10 week vitamin E supplementation period, these exercise-induced changes in PON1 activity, EMF and MDA concentration were still significant in the control group, but not in the supplemented group. These results suggested that vitamin E supplementation had a protective effect on submaximal exercise-induced oxidative damage in sedentary dogs.
authors.affiliation: Physiology and Therapeutic Department, National Veterinary School of Lyon, 1, Avenue Bourgelat, 69280 Marcy l'Etoile, France.
authors.forename: S
authors.initials: S
authors.lastname: Motta
authors.forename: C
authors.initials: C
authors.lastname: Letellier
authors.forename: M
authors.initials: M
authors.lastname: Ropert
authors.forename: C
authors.initials: C
authors.lastname: Motta
authors.forename: J J
authors.initials: JJ
authors.lastname: Thiébault
mesh_terms: D000818:Animals; D043303:Aryldialkylphosphatase / Q000378:metabolism*; D015415:Biomarkers; D003402:Creatine Kinase / Q000378:metabolism; D019587:Dietary Supplements; D004285:Dogs; D004910:Erythrocyte Membrane / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D010805:Physical Conditioning, Animal; D014810:Vitamin E / Q000494:pharmacology*
chemical_list: D015415:Biomarkers; D014810:Vitamin E; D008315:Malondialdehyde; D003402:Creatine Kinase; D043303:Aryldialkylphosphatase