Characterization of the Rab7K157N mutant protein associated with Charcot-Marie-Tooth type 2B.
Four missense mutations, that target highly conserved amino acid residues in the small GTPase Rab7, have been associated with the Charcot-Marie-Tooth (CMT) type 2B phenotype. CMT2B peripheral axonal neuropathies are characterized by severe sensory loss, often complicated by infections, arthropathy, and amputations. Here, we have investigated the biochemical and functional properties of the Rab7 K157N mutated protein. Interestingly, Rab7 K157N showed altered nucleotide exchange rate and GTP hydrolysis compared to the wild type protein. Consistently, the majority of the expressed protein in HeLa cells was bound to GTP. In addition, Rab7 K157N was able to restore EGF degradation, previously inhibited by Rab7 silencing. Altogether these data indicate that Rab7 K157N, similarly to the other three mutated proteins causative of CMT2B, is predominantly in the GTP-bound form and behaves as an active mutant. Therefore, activated forms of Rab7 protein cause the CMT2B disease.
De Luca
Azzurra
A
Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali (DiSTeBA), Università del Salento, Via Provinciale Monteroni, 73100 Lecce, Italy.
Progida
Cinzia
C
Spinosa
Maria Rita
MR
Alifano
Pietro
P
Bucci
Cecilia
C
D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism; D000595:Amino Acid Sequence; D001216:Asparagine / Q000737:chemistry / Q000235:genetics; D018797:Cell Cycle Proteins / Q000378:metabolism; D002607:Charcot-Marie-Tooth Disease / Q000201:enzymology* / Q000235:genetics; D017124:Conserved Sequence; D066246:ErbB Receptors / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D008239:Lysine / Q000737:chemistry / Q000235:genetics; D020125:Mutation, Missense; D009687:Nuclear Proteins / Q000378:metabolism; D034622:RNA Interference; D020691:rab GTP-Binding Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D000090802:rab7 GTP-Binding Proteins
D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; C470309:MAD2L1BP protein, human; D009687:Nuclear Proteins; D000090802:rab7 GTP-Binding Proteins; C000716463:rab7 GTP-binding proteins, human; D001216:Asparagine; D006160:Guanosine Triphosphate; D066246:ErbB Receptors; D020691:rab GTP-Binding Proteins; D008239:Lysine