title: Letter: characterisation and identification of spermine and spermidine derivatives in Microdesmis keayana and Microdesmis puberula roots by electrospray ionisation tandem mass spectrometry and high-performance liquid chromatography/electrospray ionisation tandem mass spectrometry.
abstract: Three new N(1),N(5),N(14)-tris(4- hydroxycinnamoyl)spermines were identified in hydromethanolic root extracts of Microdesmis keayana J. Léonard and Microdesmis puberula Hook f. The electrospray ionisation tandem mass spectrometry (ESI-MS/MS) technique with specific nuclear magnetic resonance analysis of hydrolysed products made it possible to identify N(1),N(5),N(14)-tris(p-coumaroyl)spermine, N(1)-feruloyl,N(5),N(14)-di(p-coumaroyl)spermine and N(1),N(5),N(14)-tris(feruloyl)spermine, named keayanines B, C and D, respectively. ESI-MS/MS analysis most effectively provided structural data although high-performance liquid chromatography/electrospray ionisation tandem mass spectrometry was also used to characterise four other compounds from Microdesmis puberula-keayanidines A, B, C and keayanine A-which had already been identified in M. keayana. This chemical data is the first to be published for M. puberula which is a commonly used plant in Central African traditional medicine.
authors.forename: Vincent
authors.initials: V
authors.lastname: Roumy
authors.forename: Thierry
authors.initials: T
authors.lastname: Hennebelle
authors.forename: Alexis
authors.initials: A
authors.lastname: Zamblé
authors.forename: Jacques
authors.initials: J
authors.lastname: Zamblé Yao
authors.forename: Sevser
authors.initials: S
authors.lastname: Sahpaz
authors.forename: François
authors.initials: F
authors.lastname: Bailleul
mesh_terms: D002853:Chromatography, Liquid; D008519:Medicine, Traditional; D018517:Plant Roots / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013095:Spermidine / Q000737:chemistry*; D013096:Spermine / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D013096:Spermine; D013095:Spermidine