Molecular cloning and expression profiles of the acyl-CoA-binding protein gene from the cotton bollworm Helicoverpa armigera.
Acyl-CoA-binding protein (ACBP), also known as the diazepam-binding inhibitor (DBI), has been identified in diverse species and is evolutionarily conserved in plants and animals. In a recent study, an ACBP cDNA (HaACBP) encoding 85 amino acids was isolated from the cotton bollworm Helicoverpa armigera. The isolated protein is highly homologous to the ACBP present in the Bombyx mori midgut, where it is highly expressed. Northern and Western blot analyses revealed that HaACBP is expressed predominantly in the midgut. Moreover, Northern blotting revealed that HaACBP was probably stimulated by a high juvenile hormone titer at ecdysis and increased along with feeding at 12 h post-ecdysis. Immunohistochemistry of the midgut revealed that HaACBP is localized in columnar cells. Data from the Northern blotting and immunohistochemistry suggested that HaACBP was expressed during the larval period and is probably responsible for nutrition absorption. However, Western blot analysis of the midgut at different developmental stages indicated that HaACBP was upregulated during larval molting and metamorphosis, which suggested that HaACBP expression was posttranscriptionally regulated.
Wang
Jia-Lin
JL
School of Life Sciences, Shandong University, Jinan, Shandong, China.
Wang
Jin-Xing
JX
Zhao
Xiao-Fan
XF
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D026261:Diazepam Binding Inhibitor / Q000302:isolation & purification / Q000378:metabolism*; D041981:Gastrointestinal Tract / Q000378:metabolism; D015870:Gene Expression; D007150:Immunohistochemistry; D019476:Insect Proteins / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D008969:Molecular Sequence Data; D009036:Moths / Q000235:genetics* / Q000378:metabolism; D010802:Phylogeny; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D026261:Diazepam Binding Inhibitor; D019476:Insect Proteins; D011994:Recombinant Proteins