Selective killing of Mycobacterium avium-infected macrophages by inhibition of phosphorylated signal transducer and activator of transcription type 1.
Mycobacterium avium infects mononuclear phagocytes, which thereby become reservoirs for this pathogen. Currently recommended therapy does not ensure the eradication of intracellular bacteria. Here, we report that M. avium infection in macrophages activates the signal transducer and activator of transcription type 1 (STAT-1) signaling pathway. Fludarabine, an antileukemic drug active against cells that express STAT-1, selectively kills M. avium-infected macrophages. These findings suggest that phosphorylated STAT-1 can enhance the survival of macrophages, promoting their role as persistent reservoirs of M. avium. This work invites research on new combination therapeutic approaches that consist of fludarabine, to kill the macrophage reservoir, and antibacterial agents, to eliminate mycobacteria released from the dead cells.
Dominici
Sabrina
S
Institutes of Biochemistry "G. Fornaini", University of Urbino "Carlo Bo," Urbino, Italy.
Brandi
Giorgio
G
Schiavano
Giuditta Fiorella
GF
Magnani
Mauro
M
D000970:Antineoplastic Agents / Q000494:pharmacology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008264:Macrophages / Q000187:drug effects / Q000382:microbiology*; D009162:Mycobacterium avium / Q000502:physiology*; D010766:Phosphorylation; D050794:STAT1 Transcription Factor / Q000037:antagonists & inhibitors*; D013997:Time Factors; D014740:Vidarabine / Q000031:analogs & derivatives* / Q000494:pharmacology
D000970:Antineoplastic Agents; D050794:STAT1 Transcription Factor; D014740:Vidarabine; C024352:fludarabine