title: Multichromosomal genome structure and confirmation of diazotrophy in novel plant-associated Burkholderia species.
abstract: Pulsed-field gel electrophoresis and 16S rRNA hybridization experiments showed that multichromosome genome structures and very large genome sizes (6.46 to 8.73 Mb) are prevalent in novel plant-associated Burkholderia species. (15)N(2) isotope dilution assays revealed unambiguous diazotrophy in these novel species. nifH gene sequence analysis, often used to determine phylogenetic relatedness among diazotrophs, showed tight clusters of Burkholderia species, which were clearly distinct from those of other diazotrophs.
authors.affiliation: Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Ap. Postal 565-A, Cuernavaca, Morelos, México.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Martínez-Aguilar
authors.forename: Rafael
authors.initials: R
authors.lastname: Díaz
authors.forename: Juan José
authors.initials: JJ
authors.lastname: Peña-Cabriales
authors.forename: Paulina
authors.initials: P
authors.lastname: Estrada-de Los Santos
authors.forename: Michael F
authors.initials: MF
authors.lastname: Dunn
authors.forename: Jesús
authors.initials: J
authors.lastname: Caballero-Mellado
mesh_terms: D001483:Base Sequence; D019117:Burkholderia / Q000145:classification* / Q000201:enzymology / Q000235:genetics*; D002876:Chromosomes, Bacterial / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification; D016521:Electrophoresis, Gel, Pulsed-Field; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009586:Nitrogen Fixation; D009693:Nucleic Acid Hybridization; D010088:Oxidoreductases / Q000235:genetics; D010802:Phylogeny; D010944:Plants / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D010088:Oxidoreductases; C029130:nitrogenase reductase