Id2, Id3, and Id4 proteins show dynamic changes in expression during vibrissae follicle development.
Id proteins are involved in the transcriptional control of many fundamental biological processes, including differentiation and lineage commitment. We studied Id2, Id3, and Id4 protein expression during different stages of rat vibrissa follicle development using immunohistochemistry. Id2 was highly expressed in the cytoplasm of specialized cells in the basal epidermis and outer root sheath during early stages of follicle development. These cells were identified as Merkel cells (MCs) by means of double-immunolabeling with synaptophysin and cytokeratin-20, and persisted in neonatal follicles. Id3 immunofluorescence was characterized by membrane-associated expression in basal epithelial cells of follicles early in development. Subsequently follicle epithelial cells switched to have strong nuclear labeling, also a feature of newly forming dermal papilla cells. Id4 expression was primarily associated with innervation of the developing follicle musculature. These observations illustrate dynamic expression patterns of Id2 and Id3 proteins in developing follicles and specifically link Id2 expression to Merkel cell specification.
Hammond
Nigel L
NL
School of Biological and Biomedical Sciences, University of Durham, South Road, Durham, United Kingdom.
Jahoda
Colin A B
CA
D000818:Animals; D000831:Animals, Newborn; D018507:Gene Expression Regulation, Developmental; D018859:Hair Follicle / Q000196:embryology*; D051797:Inhibitor of Differentiation Protein 2 / Q000502:physiology*; D051796:Inhibitor of Differentiation Proteins / Q000502:physiology*; D053554:Keratin-20 / Q000096:biosynthesis; D018862:Merkel Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence; D051381:Rats; D017208:Rats, Wistar; D016708:Synaptophysin / Q000096:biosynthesis; D014018:Tissue Distribution; D014738:Vibrissae / Q000196:embryology*
C497451:Id2 protein, rat; C499848:Id3 protein, rat; C490126:Id4 protein, rat; D051797:Inhibitor of Differentiation Protein 2; D051796:Inhibitor of Differentiation Proteins; D053554:Keratin-20; D016708:Synaptophysin