title: Glucose/oxygen deprivation and reperfusion upregulate SNAREs and complexin in organotypic hippocampal slice cultures.
abstract: Brain ischemia activates Ca(2+)-dependent synaptic vesicle exocytosis. The synaptosomal-associated protein 25 (SNAP-25) and syntaxin proteins, located on presynaptic terminals, are components of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex and play a key role in regulating exocytosis. Changes in the expression of SNAREs could affect SNARE complex formation, fusion of vesicles with the presynaptic membrane, and release of neurotransmitters through exocytosis. To investigate the relationship of glucose/oxygen deprivation (GOD)/reperfusion-induced neuronal damage and alteration of presynaptic function, we examined the expression of SNAREs and complexin during GOD and reperfusion using organotypic hippocampal slice cultures. Microtubule-associated protein 2 (MAP-2) staining and transmission electron microscopy showed that neuronal damage increased in a time-dependent manner and both types of neuronal death can occur at different times during GOD and reperfusion. The immunoreactivity of SNAREs such as SNAP-25, vesicle-associated membrane protein and syntaxin and complexin increased in pyramidal cell bodies in the CA1 and CA3 areas in a time-dependent manner following reperfusion. Our data suggest that alteration of presynaptic function may play a partial role in delayed neuronal death during GOD and reperfusion in organotypic hippocampal slice cultures.
authors.affiliation: Department of Pharmacology, School of Medicine, Ewha Woman's University, Yangcheon-Gu, Seoul, South Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Park
authors.forename: Yeon Joo
authors.initials: YJ
authors.lastname: Jung
authors.forename: Yul A
authors.initials: YA
authors.lastname: Kim
authors.forename: Ji Hee
authors.initials: JH
authors.lastname: Lee-Kang
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Lee
mesh_terms: D000818:Animals; D016923:Cell Death / Q000502:physiology; D015687:Cell Hypoxia; D005455:Fluorescent Antibody Technique; D006624:Hippocampus / Q000378:metabolism* / Q000648:ultrastructure; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism* / Q000473:pathology; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D017966:Pyramidal Cells / Q000378:metabolism* / Q000648:ultrastructure; D050765:Qa-SNARE Proteins / Q000378:metabolism; D050683:R-SNARE Proteins / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D050600:SNARE Proteins / Q000378:metabolism*; D050825:Synaptosomal-Associated Protein 25 / Q000378:metabolism; D046509:Tissue Culture Techniques; D015854:Up-Regulation
chemical_list: D009419:Nerve Tissue Proteins; D050765:Qa-SNARE Proteins; D050683:R-SNARE Proteins; D050600:SNARE Proteins; C494210:Snap25 protein, rat; D050825:Synaptosomal-Associated Protein 25