Interactions between PBEF and oxidative stress proteins--a potential new mechanism underlying PBEF in the pathogenesis of acute lung injury.
Identification of pre-B-cell colony-enhancing factor (PBEF) interacting partners may reveal new molecular mechanisms of PBEF in the pathogenesis of acute lung injury (ALI). The interactions between PBEF and NADH dehydrogenase subunit 1(ND1), ferritin light chain and interferon induced transmembrane 3 (IFITM3) in human pulmonary vascular endothelial cells were identified and validated. ND1, ferritin and IFITM3 are involved in oxidative stress and inflammation. Overexpression of PBEF increased its interactions and intracellular oxidative stress, which can be attenuated by rotenone. The interaction modeling between PBEF and ND1 is consistent with the corresponding experimental finding. These interactions may underlie a novel role of PBEF in the pathogenesis of ALI.
Zhang
Li Qin
LQ
Department of Surgery, University of Missouri, Columbia, MO, USA.
Adyshev
Djanybek M
DM
Singleton
Patrick
P
Li
Hailong
H
Cepeda
Javier
J
Huang
Sheng-You
SY
Zou
Xiaoqin
X
Verin
Alexander D
AD
Tu
Jiancheng
J
Garcia
Joe G N
JG
Ye
Shui Qing
SQ
D000595:Amino Acid Sequence; D016207:Cytokines / Q000737:chemistry / Q000378:metabolism*; D005293:Ferritins / Q000378:metabolism*; D015723:Gene Library; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D054409:Nicotinamide Phosphoribosyltransferase / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012128:Respiratory Distress Syndrome / Q000209:etiology* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
D016207:Cytokines; C080082:IFITM3 protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; D005293:Ferritins; D009245:NADH Dehydrogenase; C515075:NADH dehydrogenase subunit 1, human; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human