[Transepicardial autologous transplantation of bone marrow mononuclear cells for acute myocardial infarction].
OBJECTIVE
To test the effect of intramyocardial injection of autologous bone marrow mononuclear cells (MNCs) in improving the cardiac function and myocardial revascularization in miniswine models of myocardial infarction.


METHODS
The miniswine models of myocardial infarction established by ligation of the anterior descending coronary artery were divided into 3 groups including a control and two MNC injection groups. Autologous bone marrow MNCs were injected via the epicardium into the infarcted area in the latter two groups at 1 and 2 weeks after the infarction, respectively. The ventricular segmental wall motion was evaluated after the treatment, and the infarcted myocardium observed with immunohistochemistry on frozen sections.


RESULTS
The left ventricular segmental wall motion differed significantly between the control and the MNC injection groups at 1 and 2 months after the treatment. CM-DiI-positive cells were detected in the infarcted myocardium where MNCs were implanted.


CONCLUSIONS
Intramyocardial injection of autologous bone marrow MNCs improves the infarcted ventricular segmental wall motion, and significantly increases the number of blood vessels in the infracted area. The transplanted cells can be integrated into the vascular walls of the capillaries and arterioles and differentiate into cardiomyocytes.
Huang
Jin-song
JS
Department of Cardiovasculelar Surgery, Guangdong Provincial People's Hospital, Guangzhou 510100, China. jasonwong32@ hotmail.com
Zheng
Shao-yi
SY
Mai
Ming-jie
MJ
Wu
Ruo-bin
RB
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D004195:Disease Models, Animal; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D009203:Myocardial Infarction / Q000473:pathology / Q000601:surgery*; D013552:Swine; D013556:Swine, Miniature; D014182:Transplantation, Autologous