Efficient gene delivery using chitosan-polyethylenimine hybrid systems.
Chitosan and chitosan derivatives have been investigated as non-viral vectors because they have several advantages, such as biocompatibility, biodegradability, low cytotoxicity and low immunogenicity. However, low transfection efficiency and low cell specificity must be solved for their use in clinical trials. In this paper, chitosan-polyethylenimine (PEI) hybrid systems such as chitosan/PEI blend and chitosan-graft-PEI are described for efficient gene delivery because the PEI has high transfection efficiency owing to a proton sponge effect and chitosan has biocompatibility. Also, hepatocyte specificity of the galactosylated chitosan is explained after combination with PEI.
Jiang
Hu-Lin
HL
Department of Agricultural Bioechnology, Seoul National University, Seoul 151-921, Korea.
Kim
Tae-Hee
TH
Kim
You-Kyoung
YK
Park
In-Young
IY
Cho
Myung-Haing
MH
Cho
Chong-Su
CS
D048271:Chitosan / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004247:DNA / Q000008:administration & dosage* / Q000737:chemistry* / Q000235:genetics; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods; D018390:Gene Targeting / Q000379:methods; D015316:Genetic Therapy / Q000379:methods; D011094:Polyethyleneimine / Q000737:chemistry*; D014162:Transfection / Q000295:instrumentation / Q000379:methods*
D020099:Coated Materials, Biocompatible; D004337:Drug Carriers; D011094:Polyethyleneimine; D004247:DNA; D048271:Chitosan