Lectin-based electrophoretic analysis of the expression of the 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 fragment in sera of patients with five different malignancies.
A 35 kDa glycoprotein whose abundance was previously demonstrated to be enhanced in sera of patients with endometrial adenocarcinoma (n = 12), was isolated from pooled sera of three of the cancer patients using champedak galactose-binding lectin affinity chromatography in the present study. Subjecting it to 2-DE and MS/MS, the glycoprotein was identified as the O-glycosylated fragment of inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4). When compared to control sera (n = 17), expression of the 35 kDa ITIH4 cleavage fragment was demonstrated to be significantly enhanced in sera of patients with breast carcinoma (n = 10), epithelial ovarian carcinoma (n = 10), and germ cell ovarian carcinoma (n = 10) but not in patients with nasopharyngeal carcinoma (n = 13) and osteosarcoma (n = 7). The lectin-based electrophoretic bioanalytical method adopted in the present study may be used to assess the physiological relevance of ITIH4 fragmentation and its correlation with different malignancies, their stages and progression.
Mohamed
Emida
E
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Abdul-Rahman
Puteri Shafinaz
PS
Doustjalali
Saeid Reza
SR
Chen
Yeng
Y
Lim
Boon-Kiong
BK
Omar
Siti Zawiah
SZ
Bustam
Anita Zarina
AZ
Singh
Vivek Ajit
VA
Mohd-Taib
Nur Aishah
NA
Yip
Cheng-Har
CH
Hashim
Onn Haji
OH
D000293:Adolescent; D000328:Adult; D000368:Aged; D000510:Alpha-Globulins / Q000096:biosynthesis*; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000097:blood; D001859:Bone Neoplasms / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D037161:Galectins; D006031:Glycosylation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism; D009375:Neoplasms, Glandular and Epithelial / Q000378:metabolism; D012516:Osteosarcoma / Q000378:metabolism; D010051:Ovarian Neoplasms / Q000378:metabolism; D037121:Plant Lectins; D053719:Tandem Mass Spectrometry
D000510:Alpha-Globulins; D014408:Biomarkers, Tumor; D037161:Galectins; D037121:Plant Lectins; C011877:inter-alpha-inhibitor