title: [Effects of Newcastle disease virus on the expression of survivin and cell cycle in human tongue squamous carcinoma TSCCa cells].
abstract: OBJECTIVE
  To investigate the effects of Newcastle disease virus (NDV) infection on the expression of survivin and cell cycle in human tongue squamous carcinoma TSCCa cells.
  
  
  METHODS
  The proliferation of TSCCa cells infected with NDV in vitro was evaluated by means of MTT assay, and survivin expression in the infected cells was detected using RT-PCR and Western blotting. Flow cytometry was performed to assess the changes in the cell apoptosis, cell cycle and cell proliferation index (PI) of the cells.
  
  
  RESULTS
  NDV infection resulted in decreased survivin expression and increased apoptosis of TSCCa cells, with reduced cell percentage in G2/M and S phases and lowered PI of the cells, showing significant differences from those of the negative control cells (P<0.05).
  
  
  CONCLUSIONS
  NDV infection can inhibit survivin expression, affect the cell cycle of TSCCa cells and induce their apoptosis.
authors.affiliation: Southern Medical University, Guangzhou 510515, China. wanxq10@hotmail.com
authors.forename: Xue-qin
authors.initials: XQ
authors.lastname: Wan
authors.forename: Guo-yi
authors.initials: GY
authors.lastname: Dai
authors.forename: Chang-wu
authors.initials: CW
authors.lastname: Dou
authors.forename: Fu-ming
authors.initials: FM
authors.lastname: Tian
authors.forename: Min
authors.initials: M
authors.lastname: Long
authors.forename: Bei-guo
authors.initials: BG
authors.lastname: Long
authors.forename: Xiao-juan
authors.initials: XJ
authors.lastname: Wang
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000821:virology; D002453:Cell Cycle / Q000502:physiology*; D045744:Cell Line, Tumor; D054884:Host-Pathogen Interactions; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D009522:Newcastle disease virus / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D014062:Tongue Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D000077022:Survivin