title: Molecular characterization of human erythrovirus B19 strains obtained from patients with several clinical presentations in the Amazon region of Brazil.
abstract: BACKGROUND
  Human erythrovirus B19, endemic in the Amazon region since 1990, is associated with a wide spectrum of clinical presentations.
  
  
  OBJECTIVE
  To assess the prevalence of erythrovirus B19 infection and the relative frequency of erythrovirus B19 genotypes in patients in the Amazon region with various clinical presentations.
  
  
  METHODS
  A total of 487 clinical samples obtained from patients with symptoms suggestive of erythrovirus infection were tested using specific IgM and IgG antibody assays (ELISA) and PCR for viral DNA detection. Partial VP1 and VP2 regions were sequenced and genotyped by phylogenetic reconstruction.
  
  
  RESULTS
  B19 DNA was detected in 117 (24%) of 487 samples. Of these, 106 (91%) isolates were genotype 1 and 11 (9%) were genotype 3. No genotype 2 was found. Genotype 1 had three clusters (A1, A2 and B) and all genotype 3 sequences were subtype 3b. All patients with hematological disorders within cluster B of genotype 1 were infected by the same B19 lineage, suggesting that this lineage of B19 may have been transmitted via transfusion of blood products.
  
  
  CONCLUSIONS
  We reported two genotypes, 1 and 3b, with three genotype 1 clusters co-circulating in the Amazon region during the past 10 years.
authors.affiliation: Seção de Virologia, Instituto Evandro Chagas, Secretaria de Vigilância em Saúde, Ministério da Saúde, Rodovia BR 316-Km 7, S/N Levilândia, 67037-000 Ananindeua, Pará, Brazil. ronaldofreitas@iec.pa.gov.br
authors.forename: Ronaldo B
authors.initials: RB
authors.lastname: Freitas
authors.forename: Fernando L
authors.initials: FL
authors.lastname: Melo
authors.forename: Darleise S
authors.initials: DS
authors.lastname: Oliveira
authors.forename: Camila M
authors.initials: CM
authors.lastname: Romano
authors.forename: Maria Rute C
authors.initials: MR
authors.lastname: Freitas
authors.forename: Olinda
authors.initials: O
authors.lastname: Macêdo
authors.forename: Alexandre C
authors.initials: AC
authors.lastname: Linhares
authors.forename: Paolo M
authors.initials: PM
authors.lastname: de A Zanotto
authors.forename: Edison Luiz
authors.initials: EL
authors.lastname: Durigon
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001499:Bayes Theorem; D001938:Brazil / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D005838:Genotype; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000821:virology*; D016732:Parvovirus B19, Human / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction
chemical_list: D004279:DNA, Viral