Immunogold for detection of avian leukosis/sarcoma virus in formalin-fixed heart and kidney.
Immunoprobes are used in the qualitative and semi-quantitative demonstration of antigens in clinical samples. In the present study, an immunogold method has been used for routine and rapid detection of avian leukosis/sarcoma virus (AL/SV) in sections of formalin-fixed chicken heart and kidney. Application of this ultrastructural indirect immunogold labelling technique (IILT) will enable pathologists to obtain a definitive diagnosis of avian AL/SV infection when inclusions or putative viral particles are observed by light or electron microscopy. IILT also may be used in research on AL/SV infections.
Steffens
W L
WL
Department of Veterinary Pathology, College of Veterinary Medicine, The University of Georgia, Athens, Georgia, USA.
Goodwin
M A
MA
Ard
M B
MB