title: Association between a polymorphism in the IL-12p40 gene and cytomegalovirus reactivation after kidney transplantation.
abstract: BACKGROUND
  Cytomegalovirus (CMV) infection is associated with a significant rate of morbidity after organ transplantation. The genetic factors influencing its occurrence have been little investigated. IL-12 plays a crucial role in anti-infectious immune responses, especially by stimulating IFNgamma production. An A-to-C single nucleotide polymorphism (SNP) within the 3'-untranslated region of the IL-12p40 gene has been characterized and was reported to be both functionally and clinically relevant. However, the impact of this single nucleotide polymorphism on events after organ transplantation has never been reported.
  
  
  METHODS
  In this study, we investigated the impact of the 3'-untranslated region polymorphism on the occurrence of CMV infection in 469 kidney recipients transplanted at the University Hospital of Tours between 1995 and 2005. The polymorphism was genotyped using the restriction fragment length polymorphism method and CMV infection was determined by pp65 antigenemia.
  
  
  RESULTS
  Multifactorial Cox regression analysis demonstrated that the presence of the C allele was an independent risk factor for CMV infection (OR=1.52, P=0.043), the risk being even higher when study was restricted to patients with positive CMV serological status before the graft and who did not receive any CMV prophylaxis (OR=1.88, P=0.028).
  
  
  CONCLUSIONS
  This study identified a new genetic risk factor for CMV reactivation after kidney transplantation. The results of our study suggest that C carriers might especially benefit from CMV prophylaxis.
authors.affiliation: JE2448 Cellules Dendritiques et Greffes, UFR de Médecine, Université François Rabelais, Tours, France.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Hoffmann
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Halimi
authors.forename: Mathias
authors.initials: M
authors.lastname: Büchler
authors.forename: Florence
authors.initials: F
authors.lastname: Velge-Roussel
authors.forename: Alain
authors.initials: A
authors.lastname: Goudeau
authors.forename: Azmi
authors.initials: A
authors.lastname: Al  Najjar
authors.forename: Marie-Denise
authors.initials: MD
authors.lastname: Boulanger
authors.forename: Tarik Sqalli
authors.initials: TS
authors.lastname: Houssaini
authors.forename: Jean-Frédéric
authors.initials: JF
authors.lastname: Marliere
authors.forename: Yvon
authors.initials: Y
authors.lastname: Lebranchu
authors.forename: Christophe
authors.initials: C
authors.lastname: Baron
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics; D000328:Adult; D003587:Cytomegalovirus / Q000502:physiology*; D003586:Cytomegalovirus Infections / Q000453:epidemiology* / Q000235:genetics; D017931:DNA Primers; D005260:Female; D005838:Genotype; D006801:Humans; D018664:Interleukin-12 / Q000235:genetics*; D007674:Kidney Diseases / Q000145:classification / Q000601:surgery; D016030:Kidney Transplantation / Q000009:adverse effects*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D014775:Virus Activation
chemical_list: D020413:3' Untranslated Regions; D017931:DNA Primers; D018664:Interleukin-12