Quantification of trans-1,4-polyisoprene in Eucommia ulmoides by fourier transform infrared spectroscopy and pyrolysis-gas chromatography/mass spectrometry.
Commercial development of trans-1,4-polyisoprene from Eucommia ulmoides Oliver (EU-rubber) requires specific knowledge on selection of high-rubber-content lines and establishment of agronomic cultivation methods for achieving maximum EU-rubber yield. The development can be facilitated by high-throughput and highly sensitive analytical techniques for EU-rubber extraction and quantification. In this paper, we described an efficient EU-rubber extraction method, and validated that the accuracy was equivalent to that of the conventional Soxhlet extraction method. We also described a highly sensitive quantification method for EU-rubber by Fourier transform infrared spectroscopy (FT-IR) and pyrolysis-gas chromatography/mass spectrometry (PyGC/MS). We successfully applied the extraction/quantification method for study of seasonal changes in EU-rubber content and molecular weight distribution.
Takeno
Shinya
S
Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Bamba
Takeshi
T
Nakazawa
Yoshihisa
Y
Fukusaki
Eiichiro
E
Okazawa
Atsushi
A
Kobayashi
Akio
A
D002070:Butadienes / Q000032:analysis; D031283:Eucommiaceae / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D045782:Hemiterpenes / Q000032:analysis / Q000096:biosynthesis; D010420:Pentanes / Q000032:analysis; D012408:Rubber / Q000032:analysis* / Q000378:metabolism*; D012621:Seasons; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods
D002070:Butadienes; D045782:Hemiterpenes; D010420:Pentanes; C005059:isoprene; D012408:Rubber