title: Biotinyl-methyl 4-(amidomethyl)benzoate is a competitive inhibitor of human biotinidase.
abstract: Posttranslational modification of histones by biotinylation can be catalyzed by both biotinidase (BTD) and holocarboxylase synthetase. Biotinylation of histones is an important epigenetic mechanism to regulate gene expression, DNA repair, and chromatin remodeling. The role of BTD in histone biotinylation is somewhat ambiguous, given that BTD also catalyzes removal of the biotin tag from histones. Here, we sought to develop BTD inhibitors for future studies of the role of BTD in altering chromatin structure. We adopted an existing colorimetric BTD assay for use in a novel 96-well plate format to permit high-throughput screening of potential inhibitors. Biotin analogs were chemically synthesized and tested for their ability to inhibit human BTD. Seven of these compounds inhibited BTD by 26-80%. Biotinyl-methyl 4-(amidomethyl)benzoate had the largest effect on BTD, causing an 80% inhibition at 1 mM concentration. Enzyme kinetics studies were conducted to determine V(max), K(m) and K(i) for the seven inhibitors; kinetics were consistent with the hypothesis that biotinyl-methyl 4-(amidomethyl)benzoate and the other compounds acted by competitive inhibition of BTD. Finally, biotinyl-methyl 4-(amidomethyl)benzoate did not affect biotin transport in human cells, suggesting specificity in regard to biotin-related processes.
authors.affiliation: Department of Nutrition and Health Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583-0806, USA.
authors.forename: Keyna A
authors.initials: KA
authors.lastname: Kobza
authors.forename: Kittichai
authors.initials: K
authors.lastname: Chaiseeda
authors.forename: Gautam
authors.initials: G
authors.lastname: Sarath
authors.forename: James M
authors.initials: JM
authors.lastname: Takacs
authors.forename: Janos
authors.initials: J
authors.lastname: Zempleni
mesh_terms: D010129:4-Aminobenzoic Acid / Q000378:metabolism; D001692:Biological Transport; D001710:Biotin / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology*; D043523:Biotinidase / Q000037:antagonists & inhibitors / Q000097:blood*; D019921:Biotinylation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006657:Histones / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics
chemical_list: D004791:Enzyme Inhibitors; D006657:Histones; D001710:Biotin; D043523:Biotinidase; D010129:4-Aminobenzoic Acid