title: Induced immune response of DNA vaccine encoding an association MSP1a, MSP1b, and MSP5 antigens of Anaplasma marginale.
abstract: The outer membrane proteins of Anaplasma marginale have been the focus of research to obtain an improved vaccine against bovine anaplasmosis. We evaluated the capacity of the recombinant plasmids pcDNA-msp1alpha, pcDNA-msp1beta, and pcDNA-mp5 to express MSP1a, MSP1b, and MSP5 proteins, and to determine the immunogenicity of BALB/c mice immunized with these plasmids individually or in association. Expression of proteins was confirmed in Vero cells by IFA. The combination of recombinant plasmids showed high antibodies response, produced better induction of Th1 response than individual plasmids, and induced significant proliferation of splenocytes. The mice sera immunized with A. marginale showed seroconversion and reacted with all native MSPs, but demonstrated predominance of the humoral IgG1 isotype and did not induce significant proliferation of splenocytes. The use of association of recombinant plasmid can be an effective strategy for the immunoprophylaxis of anaplasmosis.
authors.affiliation: Protozoology Laboratory, Departamento de Medicina Veterinária Preventiva, CCA, Universidade de Londrina-UEL, Londrina, Paraná State, Brazil.
authors.forename: Flora S
authors.initials: FS
authors.lastname: Kano
authors.forename: Katia
authors.initials: K
authors.lastname: Tamekuni
authors.forename: Adriana L
authors.initials: AL
authors.lastname: Coelho
authors.forename: João Luis
authors.initials: JL
authors.lastname: Garcia
authors.forename: Odilon
authors.initials: O
authors.lastname: Vidotto
authors.forename: Eiko Nakagawa
authors.initials: EN
authors.lastname: Itano
authors.forename: Marilda C
authors.initials: MC
authors.lastname: Vidotto
mesh_terms: D042322:Anaplasma marginale / Q000235:genetics / Q000276:immunology*; D000712:Anaplasmosis / Q000517:prevention & control*; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics / Q000276:immunology*; D002417:Cattle; D002418:Cattle Diseases / Q000517:prevention & control; D049109:Cell Proliferation; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D013154:Spleen / Q000276:immunology; D019444:Vaccines, DNA / Q000235:genetics / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D007074:Immunoglobulin G; D019444:Vaccines, DNA; C449089:major surface protein 1a, Anaplasma; C450434:major surface protein 1b, Anaplasma; C111578:major surface protein 5, Anaplasma