Authentication of the traditional medicinal plant Eleutherococcus senticosus by DNA and chemical analyses.
Shigoka (SGK), the rhizome of Eleutherococcus senticosus, is a traditional medicine used as a tonic in northeastern Asia and far eastern Russia. We analyzed the nuclear ribosomal DNA internal transcribed spacer (ITS) sequence of the medicine available on the Japanese and Chinese markets and found that at least 3 species were used as the source plant of the commercial SGKs and that only 70% of all samples was made from the correct species. Furthermore, we performed the quantitative determination of 3 marker compounds, eleutheroside B (EB), syringaresinol diglucoside (Syr), and isofraxidin (Iso) by ultraperformance liquid chromatography (UPLC)/mass spectrometry (MS). We found that EB and Iso are specific to the correct source plant of SGK. Of them, EB is thought to be the best marker compound for quality assurance of the SGK from the viewpoint of its pharmacological activity.
Maruyama
Takuro
T
Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan.
Kamakura
Hiroyuki
H
Miyai
Miho
M
Komatsu
Katsuko
K
Kawasaki
Takeshi
T
Fujita
Masao
M
Shimada
Hiroshi
H
Yamamoto
Yutaka
Y
Shibata
Toshiro
T
Goda
Yukihiro
Y
D003374:Coumarins / Q000032:analysis; D021903:DNA, Ribosomal Spacer; D028001:Eleutherococcus / Q000737:chemistry / Q000235:genetics*; D005960:Glucosides / Q000032:analysis; D017705:Lignans / Q000032:analysis; D010666:Phenylpropionates / Q000032:analysis; D028321:Plant Preparations / Q000737:chemistry / Q000592:standards; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics*; D027343:Rhizome / Q000737:chemistry / Q000235:genetics
D003374:Coumarins; D021903:DNA, Ribosomal Spacer; D005960:Glucosides; D017705:Lignans; D010666:Phenylpropionates; D028321:Plant Preparations; C407309:syringaresinol-4''-O-glucoside; C008182:isofraxidin; C028305:syringin