A new purple fluorescent color marker for genetic studies in Saccharomyces cerevisiae and Candida albicans.
The ability to visualize cellular events by linking them to color or fluorescence changes has been an invaluable tool for biology. We describe a novel plasmid-borne color marker whose expression in yeast leads to purple-colored cells that are also brightly fluorescent. This dominant marker provides a useful tool for rapidly screening plasmid maintenance using a visual or fluorescence assay in both Saccharomyces cerevisiae and Candida albicans.
Keppler-Ross
Sabine
S
Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA.
Noffz
Christine
C
Dean
Neta
N
D001483:Base Sequence; D002176:Candida albicans / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006651:Histocytochemistry / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D017422:Sequence Analysis, DNA; D000097573:Red Fluorescent Protein
D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein