Molecular dynamics and forces of a motile cell simultaneously visualized by TIRF and force microscopies.
Cells must exert traction forces onto the substratum for continuous migration. Molecular dynamics such as actin polymerization at the front of the cell and myosin II accumulation at the rear should play important roles in the exertion of forces required for migration. Therefore, it is important to reveal the relationships between the traction forces and molecular dynamics. Traction forces can be calculated from the deformation of the elastic substratum under a migrating cell. A transparent and colorless elastic substratum with a high refractive index (1.40) and a low Young's modulus (1.0 kPa) were made from a pair of platinum-catalyzed silicones. We used this substratum to develop a new method for simultaneous recording of molecular dynamics and traction forces under a migrating cell in which total internal refractive fluorescence (TIRF) and force microscopies were combined. This new method allows the detection of the spatiotemporal distribution of traction forces produced by individual filopodia in migrating Dictyostelium cells, as well as simultaneous visualization of these traction forces and the dynamics of filamentous myosin II.
Iwadate
Yoshiaki
Y
Department of Functional Molecular Biology, Graduate School of Medicine, Yamaguchi University, Yamaguchi, Japan. iwadate@yamaguchi-u.ac.jp
Yumura
Shigehiko
S
D000818:Animals; D002465:Cell Movement / Q000502:physiology*; D004023:Dictyostelium / Q000502:physiology* / Q000648:ultrastructure*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D012031:Refractometry / Q000379:methods; D013314:Stress, Mechanical