title: Plasma carbonyls do not correlate with lung function or computed tomography measures of lung density in older smokers.
abstract: Oxidative stress and inflammation are hallmarks of chronic obstructive pulmonary disease (COPD). A critical byproduct of oxidative damage is the introduction of carbonyl groups into amino acid residues. We hypothesize that plasma carbonyl content is inversely correlated with lung function and computed tomography (CT) measures of lung density among smokers and is elevated in COPD. Carbonyl was measured in plasma of participants aged 60 years and older by ELISA. Generalized linear and additive models were used to adjust for potential confounders. Among 541 participants (52% male, mean age 67 years, 41% current smokers), mean plasma carbonyl content was 17.9+/-2.9 nmol ml(-1) and mean forced expiratory volume in one second (FEV(1)) was 80.7+/-20.9% of predicted. Plasma carbonyl content was inversely associated with FEV(1), but this relationship was largely explained by age. Multivariate analyses ruled out clinically meaningful associations of plasma carbonyl content with FEV(1), FEV(1)/FVC (forced vital capacity) ratio, severity of airflow obstruction, and CT lung density. Plasma carbonyl content is a poor biomarker of oxidative stress in COPD and emphysema.
authors.affiliation: Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.
authors.forename: Sonia
authors.initials: S
authors.lastname: Mesia-Vela
authors.forename: Chih-Ching
authors.initials: CC
authors.lastname: Yeh
authors.forename: John H M
authors.initials: JH
authors.lastname: Austin
authors.forename: Matthew
authors.initials: M
authors.lastname: Dounel
authors.forename: Charles A
authors.initials: CA
authors.lastname: Powell
authors.forename: Anthony
authors.initials: A
authors.lastname: Reeves
authors.forename: Regina M
authors.initials: RM
authors.lastname: Santella
authors.forename: Lori
authors.initials: L
authors.lastname: Stevenson
authors.forename: David
authors.initials: D
authors.lastname: Yankelevitz
authors.forename: R Graham
authors.initials: RG
authors.lastname: Barr
mesh_terms: D000367:Age Factors; D000368:Aged; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005541:Forced Expiratory Volume; D006801:Humans; D008168:Lung / Q000473:pathology* / Q000503:physiopathology*; D008297:Male; D008875:Middle Aged; D050050:Protein Carbonylation; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood / Q000473:pathology / Q000503:physiopathology; D011656:Pulmonary Emphysema / Q000097:blood / Q000473:pathology / Q000503:physiopathology; D012129:Respiratory Function Tests; D012907:Smoking / Q000097:blood* / Q000473:pathology / Q000503:physiopathology; D014057:Tomography, X-Ray Computed; D014797:Vital Capacity
chemical_list: D015415:Biomarkers; D001798:Blood Proteins