[Usefulness of the determination of IgG and IgM antibodies by ELISA and immunocapture in a clinical series of human brucellosis].
BACKGROUND
The diagnostic difficulties of brucellosis makes the evaluation of new diagnostic tests necessary.


OBJECTIVE
Evaluation of different commercial tests in the serological diagnosis of brucellosis by ELISA and immunocapture antibodies in a clinical series of patients with brucellosis of the Health Network of the Catholic University of Chile.


METHODS
All the serums received in the Laboratory of Microbiology for suspicion of brucellosis during five years were studied. Two groups were obtained, one that fulfilled diagnostic criteria for brucellosis [clinical evidence, and/or positive blood culture and/or seroagglutination test (SAT) in titers > 1/160] and the control group. Each serum sample was analyzed using irnmunocapture-agglutination test (Brucellacapt), ELISA IgM and IgG.


RESULTS
Of 10 patients with brucellosis, the serologic results were: 8/10 positives for ELISA IgG, 7/10 for Brucellacapt and SAT, and 5/10 for ELISA IgM.


CONCLUSIONS
ELISA IgG alone was superior than SAT. The combination ELISA IgG/ Brucellacapt reaches the best detection performance (9/10) and can be an alternative to SAT.
Aranís J
Carolina
C
Departamento de Laboratorios Clínicos, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
Oporto C
Jorge
J
Espinoza
Mónica
M
Riedel K
Ingrid
I
Pérez C
Carlos
C
García C
Patricia
P
D000208:Acute Disease; D000328:Adult; D000368:Aged; D000372:Agglutination Tests / Q000379:methods*; D002002:Brucella / Q000276:immunology*; D002006:Brucellosis / Q000175:diagnosis* / Q000276:immunology; D016022:Case-Control Studies; D002908:Chronic Disease; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D012189:Retrospective Studies
D007074:Immunoglobulin G; D007075:Immunoglobulin M