Recent advances in immobilization methods of antibodies on solid supports.
Antibody immobilization on a solid support is an essential process for the development of most immune-based assay systems. The choice of the immobilization method greatly affects antibody-antigen interactions on the assay surface. For the past several years, numerous strategies have been reported to control antibody immobilization, mainly by directing the orientation, stability, and density of bound antibodies on different assay platforms. Here we discuss recent developments in antibody immobilization methods with a particular focus on the strengths and limitations of reported approaches, and thereby provide a useful guideline for the selection of suitable antibody coupling procedures.
Jung
Yongwon
Y
BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, P.O. Box 115, Yuseong, Daejeon 305-600, Korea.
Jeong
Jin Young
JY
Chung
Bong Hyun
BH
D000327:Adsorption; D000911:Antibodies, Monoclonal; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000032:analysis*; D006801:Humans; D007159:Immunologic Tests / Q000379:methods*
D000911:Antibodies, Monoclonal; D000941:Antigens