Production of (S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase.
(S)-2-Chloropropionate is a synthetic intermediate for phenoxypropionic acid herbicides. We constructed a system for asymmetric reduction of 2-chloroacrylate to produce (S)-2-chloropropionate with recombinant Escherichia coli cells producing 2-haloacrylate reductase from Burkholderia sp. WS and an NADPH regeneration system. The system provided 37.4 g/l (S)-2-chloropropionate in more than 99.9%e.e.
Kurata
Atsushi
A
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
Fujita
Michiyo
M
Mowafy
Amr M
AM
Kamachi
Harumi
H
Kurihara
Tatsuo
T
Esaki
Nobuyoshi
N
D000179:Acrylates / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D019117:Burkholderia / Q000201:enzymology* / Q000235:genetics; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics; D042843:Glucose 1-Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D006843:Hydrocarbons, Chlorinated; D010084:Oxidation-Reduction; D011422:Propionates / Q000378:metabolism*
C502742:2-chloroacrylic acid; D000179:Acrylates; D001426:Bacterial Proteins; D006843:Hydrocarbons, Chlorinated; D011422:Propionates; C021974:2-chloropropionic acid; D042843:Glucose 1-Dehydrogenase