title: Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells.
abstract: Tumblerlike magnetic/fluorescein isothiocyanate (FITC)-labeled mesoporous silica nanoparticles, Mag-Dye@MSNs, have been developed, which are composed of silica-coated core-shell superparamagnetic iron oxide (SPIO@SiO(2)) nanoparticles co-condensed with FITC-incorporated mesoporous silica. Mag-Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and in vivo, as manifested by using a clinical 1.5-T MRI system with requirements of simultaneous low incubation dosage of iron, low detection cell numbers, and short incubation time. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes, which can still be readily detected by MRI. Moreover, a higher MRI signal intensity decrease is observed in Mag-Dye@MSN-treated cells than in SPIO@SiO(2)-treated cells. This is the first report that MCM-41-type MSNs are advantageous to cellular uptake, as manifested by a higher labeling efficiency of Mag-Dye@MSNs than SPIO@SiO(2).
authors.affiliation: Department of Medical Imaging, National Taiwan University Hospital and College of Medicine National Taiwan University, Taipei, Taiwan.
authors.forename: Hon-Man
authors.initials: HM
authors.lastname: Liu
authors.forename: Si-Han
authors.initials: SH
authors.lastname: Wu
authors.forename: Chen-Wen
authors.initials: CW
authors.lastname: Lu
authors.forename: Ming
authors.initials: M
authors.lastname: Yao
authors.forename: Jong-Kai
authors.initials: JK
authors.lastname: Hsiao
authors.forename: Yann
authors.initials: Y
authors.lastname: Hung
authors.forename: Yu-Shen
authors.initials: YS
authors.lastname: Lin
authors.forename: Chung-Yuan
authors.initials: CY
authors.lastname: Mou
authors.forename: Chung-Shi
authors.initials: CS
authors.lastname: Yang
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Huang
authors.forename: Yao-Chang
authors.initials: YC
authors.lastname: Chen
mesh_terms: D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008280:Magnetics; D008422:Materials Testing; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods; D013234:Stem Cells / Q000166:cytology*; D013499:Surface Properties
chemical_list: D003287:Contrast Media; D004337:Drug Carriers; D046911:Macromolecular Substances; D012822:Silicon Dioxide