title: Salivary endothelin and vascular disorders in vibration-exposed workers.
abstract: OBJECTIVE
  This study investigated the relation between salivary endothelin, vibration exposure, and vascular disorders in a group of forestry workers.
  
  
  METHODS
  Altogether 54 forestry workers and 52 controls underwent a medical examination and a cold test with measurement of the percentage of change in finger systolic blood pressure after finger cooling from 30 degrees C to 10 degrees C (FSBP% (10 degrees)). Salivary endothelin concentration (ET(1-21), in fmol/ml) was measured by a commercially available enzyme-linked immunosorbent assay before and after the cold challenge. The anamnestic diagnosis of vibration-induced white finger (VWF), assisted by color charts, was based on the Stockholm Workshop criteria.
  
  
  RESULTS
  Six forestry workers (11%) and one control (2%) reported white fingers. Before the cold challenge, the salivary ET(1-21) concentration was significantly greater in the VWF workers than in the controls (P=0.036). The cold response of digital arteries was stronger in the VWF workers than in the controls (P<0.001) and the asymptomatic forestry workers (P=0.008). After the cold test, there was a small, not significant, increase in the salivary ET(1-21) concentration in both the controls and the forestry workers. For the latter, the salivary ET(1-21) concentration was significantly associated with both daily and total operating time with vibrating tools. A significant inverse relation between FSBP% (10 degrees )and the salivary ET(1-21) concentration was observed for the forestry workers with an abnormal cold response in their digital arteries.
  
  
  CONCLUSIONS
  This study showed an association between salivary ET(1-21) concentration, daily and cumulative vibration exposure, and vascular disorders in the fingers of professional forestry workers. Since ET(1-21) can induce powerful and long-lasting constriction of human vessels, these findings suggest a possible role of this vasopeptide in the pathogenesis of VWF.
authors.affiliation: Clinical Unit of Occupational Medicine, Department of Public Health Sciences, University of Trieste, Centro Tumori, Via della Pietà 19, Trieste, Italy. bovenzi@units.it
authors.forename: Massimo
authors.initials: M
authors.lastname: Bovenzi
authors.forename: Flavia
authors.initials: F
authors.lastname: D'Agostin
authors.forename: Francesca
authors.initials: F
authors.lastname: Rui
authors.forename: Luigi
authors.initials: L
authors.lastname: Ambrosi
authors.forename: Roberto
authors.initials: R
authors.lastname: Zefferino
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D016232:Endothelins / Q000378:metabolism*; D016468:Forestry; D053421:Hand-Arm Vibration Syndrome / Q000453:epidemiology / Q000378:metabolism* / Q000517:prevention & control; D006801:Humans; D007558:Italy / Q000453:epidemiology; D016014:Linear Models; D008297:Male; D015999:Multivariate Analysis; D009784:Occupational Diseases / Q000453:epidemiology / Q000378:metabolism* / Q000517:prevention & control; D010991:Plethysmography; D012463:Saliva
chemical_list: D015415:Biomarkers; D016232:Endothelins