title: Acetone powder from dormant seeds of Ricinus communis L: lipase activity and presence of toxic and allergenic compounds.
abstract: The influence of several factors on the hydrolytic activity of lipase, present in the acetone powder from dormant castor seeds (Ricinus communis) was evaluated. The enzyme showed a marked specificity for short-chain substrates. The best reaction conditions were an acid medium, Triton X-100 as the emulsifying agent and a temperature of 30 degrees C. The lipase activity of the acetone powder of different castor oil genotypes showed great variability and storage stability of up to 90%. The toxicology analysis of the acetone powder from genotype Nordestina BRS 149 showed a higher ricin (toxic component) content, a lower 2S albumin (allergenic compound) content, and similar allergenic potential compared with untreated seeds.
authors.affiliation: Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
authors.forename: Elisa D C
authors.initials: ED
authors.lastname: Cavalcanti
authors.forename: Fábio M
authors.initials: FM
authors.lastname: Maciel
authors.forename: Pierre
authors.initials: P
authors.lastname: Villeneuve
authors.forename: Regina C A
authors.initials: RC
authors.lastname: Lago
authors.forename: Olga L T
authors.initials: OL
authors.lastname: Machado
authors.forename: Denise M G
authors.initials: DM
authors.lastname: Freire
mesh_terms: D055516:2S Albumins, Plant; D000096:Acetone / Q000737:chemistry*; D000485:Allergens / Q000737:chemistry*; D052179:Antigens, Plant / Q000737:chemistry*; D004789:Enzyme Activation; D004795:Enzyme Stability; D008049:Lipase / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D011208:Powders; D012276:Ricin / Q000737:chemistry*; D012278:Ricinus / Q000201:enzymology*; D012639:Seeds / Q000201:enzymology*
chemical_list: D055516:2S Albumins, Plant; C541551:2S storage protein, Ricinus communis; D000485:Allergens; D052179:Antigens, Plant; D010940:Plant Proteins; D011208:Powders; D000096:Acetone; D012276:Ricin; D008049:Lipase