title: Usefulness of Mycobacterium tuberculosis molecular typing in a tuberculosis low-endemic agro-industrial setting of Brazil.
abstract: To highlight the transmission and major phylogenetic clades of Mycobacterium tuberculosis, a retrospective study was carried out at two health facilities in a small agro-industrial area in Sao Paulo, Brazil, that has a low tuberculosis incidence rate. IS6110-RFLP and spoligotyping were performed on the isolates, with the former revealing that 31.3% (35/112) of strains were clustered. Epidemiological links were found in 16 of the 35 clustered patients and were associated with transmission among patients living in public housing. Spoligotyping grouped 62.8% of the strains. The T genetic family predominated among the isolates. Of interest is that five strains had a pattern characteristic of African or Asian origin (ST535), and two others were of the rare localized type ST1888 (BRA, VEN). In addition, three new types--1889, 1890, and 1891--were identified. Spoligotyping showed that some ST may be circulating to or from Brazil, and RFLP revealed ongoing transmission in inadequately ventilated public-housing buildings. This may point to a failure in tuberculosis control policy.
authors.affiliation: Faculdade de Ciencias Farmaceuticas, Universidade Estadual Paulista, Sao Paulo, Brazil.
authors.forename: Ana Carolina
authors.initials: AC
authors.lastname: Malaspina
authors.forename: Hebe Rodrigues
authors.initials: HR
authors.lastname: Cavalcanti
authors.forename: Clarice Queico Fujimaro
authors.initials: CQ
authors.lastname: Leite
authors.forename: Silvia Maria Almeida
authors.initials: SM
authors.lastname: Machado
authors.forename: Brunilde Helena Jung
authors.initials: BH
authors.lastname: Viana
authors.forename: Rosangela Maria Gaspareto
authors.initials: RM
authors.lastname: Silva
authors.forename: Eduardo Ferraz
authors.initials: EF
authors.lastname: Hage
authors.forename: Walter Machado
authors.initials: WM
authors.lastname: Figueiredo
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Marques
authors.forename: Lucilaine
authors.initials: L
authors.lastname: Ferrazoli
authors.forename: Marcos
authors.initials: M
authors.lastname: Arbex
authors.forename: Marcio
authors.initials: M
authors.lastname: Lessi
authors.forename: Leila S
authors.initials: LS
authors.lastname: Fonseca
authors.forename: Leen
authors.initials: L
authors.lastname: Rigouts
authors.forename: Maria Helena Féres
authors.initials: MH
authors.lastname: Saad
mesh_terms: D001938:Brazil / Q000453:epidemiology; D004251:DNA Transposable Elements; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D009841:Oligonucleotides / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D011638:Public Housing; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D004251:DNA Transposable Elements; D009841:Oligonucleotides