[Study of a serum protein fingerprint diagnostic model in patients with hypertensive disorder complicating pregnancy].
OBJECTIVE
To analyze the alterations of serum protein fingerprint in patients with hypertensive disorder complicating pregnancy (HDCP), screen serum biomarker and establish a diagnostic model of HDCP.


METHODS
Surface-enhanced laser desorption lionization-time of flight-mass spectrometry (SELDI-TOF-MS) technology was used to analyze serum including 25 cases of HDCP patients and 30 cases of age-, gravity- and parity-matched healthy term pregnant women on IMAC3-Cu proteinchip before delivery. Biomarker Wizard and Biomarker Pattern software was used to detect protein peaks significantly different between HDCP and controls, and establish a primary diagnostic model of HDCP. This model was further evaluated by blind test using other 16 parts of serum protein fingerprint.


RESULTS
Ten protein peaks were significantly different at the molecular range of 2000-50000 (P < 0.01). A diagnostic model consisting of 5 protein peaks(39 837,6196,15 529, 43 248 and 22 292) was established with 100% (25/25) sensitivity, 90% (27/30) specificity, 89% (25/28) positive predictive value and 100% (27/27) negative predictive value. Blind test generated a sensitivity of 75% and specificity of 75% respectively.


CONCLUSIONS
Many differential expressed proteins exist in serum of patients with HDCP. The diagnostic model consisting of 5 protein peaks has relative high sensitivity and specificity, and can discriminate HDCP from healthy pregnant
gong
Li-yun
LY
Department of Obstetrics and Gynecology, Chaoyang Hospital, Capital Medical University, Beijing, China.
Zhang
Zhen-yu
ZY
Zheng
Yan-hua
YH
Zhang
Jian-zhong
JZ
D000328:Adult; D001798:Blood Proteins / Q000032:analysis*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000150:complications*; D010449:Peptide Mapping / Q000379:methods; D011247:Pregnancy; D011248:Pregnancy Complications / Q000209:etiology*; D040081:Protein Array Analysis / Q000379:methods; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D055815:Young Adult
D001798:Blood Proteins