Beta-adrenoceptor subtype expression in human placenta and umbilical arteries in normal and preeclamptic pregnancies.
OBJECTIVE
Preeclampsia is characterised by an abnormal vascular response to placentation and is associated with increased systemic vascular resistance and endothelial cell dysfunction. This study investigated the mRNA and protein expression of the beta(2) and beta(3)-adrenoceptors (beta-ARs) in placenta, and umbilical arteries, from preeclamptic and normotensive patients, to determine if the presence of preeclampsia altered the expression of either receptor.


METHODS
RT-PCR was used to identify beta(2)-AR and beta(3)-AR mRNA transcripts in the human placenta and in human umbilical arteries. Real-time RT-PCR was performed on total RNA from normal and preeclamptic placentae and umbilical arteries. Western blotting using antibodies for beta(2)-AR, beta(3)-AR, and beta-actin was performed on total protein isolated from preeclamptic and normotensive placentae.


RESULTS
There was no significant difference in mRNA expression levels of beta(2)-AR and beta(3)-AR between normal and preeclamptic tissues (p > 0.05). No significant difference was observed in protein levels of beta(2)-AR and beta(3)-AR between placentae from normal and preeclamptic patients (p > 0.05).


CONCLUSIONS
Aberrations in the beta-adrenoceptor signalling systems, rather than in the regulation of expression of these receptors may occur in preeclampsia, as is the case in other hypertensive disorders.
Hynes
Paul G
PG
Department of Obstetrics and Gynaecology, National University of Ireland, Galway, Ireland.
Friel
Anne M
AM
Smith
Terry J
TJ
Morrison
John J
JJ
D000328:Adult; D015153:Blotting, Western; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D006801:Humans; D010920:Placenta / Q000378:metabolism*; D011225:Pre-Eclampsia / Q000378:metabolism*; D011247:Pregnancy; D011943:Receptors, Adrenergic, beta / Q000096:biosynthesis*; D018343:Receptors, Adrenergic, beta-2 / Q000096:biosynthesis; D022702:Receptors, Adrenergic, beta-3 / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014469:Umbilical Arteries / Q000378:metabolism*
D011943:Receptors, Adrenergic, beta; D018343:Receptors, Adrenergic, beta-2; D022702:Receptors, Adrenergic, beta-3