Changes of isoflavones during the growth cycle of Lupinus albus.
The objective of this study was to monitor the changes in isoflavone content in different plant organs (leaves, stems, roots) during the crop growth stage of three cultivars of Lupinus albus (white lupin) under field conditions, taking into account sowing time effects (autumn and early spring) and cultivar effects. Three sampling dates (from late vegetative to late grain growth stages) were evaluated. Seven isoflavones and four flavonoids were identified by LC-ESI-MS analysis. The isoflavone content was higher in leaves than in stems, and it was highest before flowering, whereas it decreased during maturity. Autumn-sown plants showed higher isoflavone content than early spring-sown plants, especially in late vegetative and early reproductive stages. Genistein 7- O-glucoside was the main isoflavone of leaves and stems in the late vegetative stages of early spring sowing, whereas genistein was the main isoflavone under autumn sowing. Variation among cultivars affected only marginally the total isoflavone content. No isoflavones were detected in seeds.
D'Agostina
Alessandra
A
Department of Agri-Food Molecular Sciences (DISMA), University of Milan, Via Celoria 2, I-20133 Milan, Italy. alessandra.dagostina@tiscali.it
Boschin
Giovanna
G
Resta
Donatella
D
Annicchiarico
Paolo
P
Arnoldi
Anna
A
D005419:Flavonoids / Q000032:analysis; D007529:Isoflavones / Q000032:analysis*; D031297:Lupinus / Q000737:chemistry* / Q000254:growth & development*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots; D018547:Plant Stems / Q000737:chemistry; D012621:Seasons
D005419:Flavonoids; D007529:Isoflavones