[Association of glucocoid receptor binding activity and heat shock protein expressions after acute lung injury in mice].
OBJECTIVE
To study the association between decreased ligand binding activity of glucocoid receptor (GR) and heat shock protein 90 (Hsp90), the molecular chaperone of GR, after acute lung injury (ALI) in mice.


METHODS
In mouse models of oleic acid-induced ALI, the levels of GR, Hsp90 and Hsp70 were dynamically observed using Western blotting, and the binding capacity and binding affinity of GR assessed with radioligand binding assay.


RESULTS
After ALI, pulmonary edema was significantly aggravated in the mice with significantly increased lung body index and lung water ratio. GR increased within 1 h after the injury, but then decreased significantly to the lowest level at 12 h after the injury, and the levels of Hsp90 and Hsp70 was increased obviously and reached the highest at 12 h. Radioligand binding assay showed that the Bmax decreased gradually and Kd value increased, and these changes were consistent with the changes of Hsp90/GR.


CONCLUSIONS
The ligand binding activity of GR is related to the changes of Hsp90 after ALI.
Zhang
Min
M
Molecular Biology Center, State Key Laboratory of Trauma, Burns, and Combined Injury, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing 400042, China.
Xiong
Ren-ping
RP
Chen
Xing-yun
XY
Li
Ping
P
Zhao
Yan
Y
Liu
Ping
P
Zhou
Yuan-guo
YG
D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism*; D000818:Animals; D001665:Binding Sites; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D008297:Male; D051379:Mice; D019301:Oleic Acid; D011869:Radioligand Assay; D011897:Random Allocation; D011965:Receptors, Glucocorticoid / Q000378:metabolism*
D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D011965:Receptors, Glucocorticoid; D019301:Oleic Acid