The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone.
Burkholderia mallei has two acyl-homoserine lactone (acyl-HSL) signal generator-receptor pairs and two additional signal receptors, all of which contribute to virulence. We show that B. mallei produces N-3-hydroxy-octanoyl HSL (3OHC8-HSL) but a bmaI3 mutant does not. Recombinant Escherichia coli expressing BmaI3 produces hydroxylated acyl-HSLs, with 3OHC8-HSL being the most abundant compound. In recombinant E. coli, BmaR3 responds to 3OHC8-HSL but not to other acyl-HSLs. These data indicate that the signal for BmaR3-BmaI3 quorum sensing is 3OHC8-HSL.
Duerkop
Breck A
BA
Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195-7242, USA.
Herman
Jake P
JP
Ulrich
Ricky L
RL
Churchill
Mair E A
ME
Greenberg
E Peter
EP
D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D042726:Burkholderia mallei / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D053038:Quorum Sensing; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; C511789:N-(3-oxooctanoyl)homoserine lactone; D011994:Recombinant Proteins; D015107:4-Butyrolactone