Mobility behaviour of amino acids on silica static phase: micelles activated separations.
A new method for separation of several amino acids using mobile phases (MPs) containing mixed micelles as well as 1-butanol and aprotic organic solvents as modifying additives has been developed. The effect of experimental factors (the ratio of MP components, nature of stationary phase) on separation of amino acids is investigated and all conditions for separation are optimized. It was found that separation of three amino acids l-lysine, l-histidine and l-tryptophan on silica gel stationary phase in the interval from micrograms to milligrams is available. The proposed method is simple and allows determining the amino acids in the linear interval of 0.1-1.5mg.
Mohammad
A
A
Analytical Research Laboratory, Department of Applied Chemistry, Faculty of Engineering and Technology, Aligarh Muslim University, Aligarh, 202002 UP, India. mohammadali4u@rediffmail.com
Gupta
Rubi
R
D000596:Amino Acids / Q000737:chemistry* / Q000302:isolation & purification; D002855:Chromatography, Thin Layer; D008823:Micelles; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*
D000596:Amino Acids; D008823:Micelles; D012822:Silicon Dioxide