[Analysis of EBV-LMP1 gene mutation in plasma of patients with nasopharyngeal carcinoma in Chongqing].
OBJECTIVE
To detect and analyze the EBV-LMP1 gene N terminus XhoI-site mutation and C terminus 30 bp deletion in plasma of patients with nasopharyngeal carcinoma (NPC) in Chongqing of China.


METHODS
DNA extraction and PCR amplification was used in plasma of 48 NPC patients and 40 control non-NPC cases from Chongqing of China. All the PCR production of N terminus was digested by enzyme XhoI, then segregated in 8% PAGE. All- the PCR production of C terminus was segregated in 8% PAGE too. Compared with B95-8 cell, some of the PCR production were sequenced and analyzed with software.


RESULTS
LMP1 was amplified successfully from 48 of 48 NPC cases (100%) and from 38 of 40 non-NPC cases (95%) by PCR, but none of LMP1 XhoI-site mutation and none of 30 bp deletion was found by digesting, segregating and Sequencing.


CONCLUSIONS
EBV-LMP1 gene N terminus XhoI-site and C terminus 30 bp deletion have no mutation in plasma of NPC or non-NPC cases in Chongqing of China. The precise relationship of EBV-LMP1 gene mutation or deletion with NPC pathogenesis needs further investigation.
Yang
Yucheng
Y
Department of Otolaryngology, the First Affiliated Hospital, Chongqing University of Medical Science, Chongqing, 400016, China.
Wang
Yiqin
Y
Qian
Di
D
He
Yun
Y
Hong
Suling
S
D000328:Adult; D002681:China; D004279:DNA, Viral / Q000235:genetics; D005260:Female; D004854:Herpesvirus 4, Human; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000097:blood / Q000235:genetics / Q000473:pathology / Q000821:virology*; D017384:Sequence Deletion; D014763:Viral Matrix Proteins / Q000097:blood* / Q000235:genetics*
D004279:DNA, Viral; C037662:EBV-associated membrane antigen, Epstein-Barr virus; D014763:Viral Matrix Proteins