Direct antimicrobial susceptibility testing method for analysis of sputum collected from patients with cystic fibrosis.
BACKGROUND
Chronic Pseudomonas aeruginosa colonisation and subsequent exacerbations in patients with cystic fibrosis (CF) require antimicrobial treatment. But since multiple morphotypes and other Gram-negative bacteria with different antibiotic susceptibilities are often isolated inside the same sputum sample, bacteriological analysis is difficult.


METHODS
To simplify this analysis, we explored a direct sputum antimicrobial susceptibility testing (DSST) method by applying E test directly on plates inoculated with the sputum. A total of 316 samples collected from CF patients were analysed and compared with standard procedures (SP) for the identification and antimicrobial susceptibility testing of all Gram-negative bacterial species.


RESULTS
DSST was as efficient as SP to detect P. aeruginosa including the mucoid morphotype in monomicrobial specimen, but was less sensible to detect all Gram-negative bacteria present in the same sample. It allowed the direct reading of the MIC inhibiting all Gram-negative bacteria. Agreements between these global MICs with the cumulative antibiotics susceptibility of all Gram-negative bacteria measured by SP were excellent for tobramycin and imipenem (>96%) and satisfactory for ticarcillin, ceftazidime, aztreonam and ciprofloxacin (90.4% to 94.3%). In conclusion, the DSST method is an efficient and easy antibiotic susceptibility testing method.
Zebouh
Mohamed
M
EA 3925, Laboratoire de Bactériologie - Hygiène, Centre de Biologie, Centre Hospitalier Universitaire, 59037 Lille, France.
Thomas
Caroline
C
Honderlick
Patrick
P
Lemee
Ludovic
L
Segonds
Christine
C
Wallet
Frédéric
F
Husson
Marie-Odile
MO
D000293:Adolescent; D000328:Adult; D003550:Cystic Fibrosis / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods*; D013183:Sputum / Q000382:microbiology*