The human cerebrospinal fluid metabolome.
With continuing improvements in analytical technology and an increased interest in comprehensive metabolic profiling of biofluids and tissues, there is a growing need to develop comprehensive reference resources for certain clinically important biofluids, such as blood, urine and cerebrospinal fluid (CSF). As part of our effort to systematically characterize the human metabolome we have chosen to characterize CSF as the first biofluid to be intensively scrutinized. In doing so, we combined comprehensive NMR, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography (LC) Fourier transform-mass spectrometry (FTMS) methods with computer-aided literature mining to identify and quantify essentially all of the metabolites that can be commonly detected (with today's technology) in the human CSF metabolome. Tables containing the compounds, concentrations, spectra, protocols and links to disease associations that we have found for the human CSF metabolome are freely available at http://www.csfmetabolome.ca.
Wishart
David S
DS
Department of Computing Science, University of Alberta, Edmonton, AB, Canada. david.wishart@ualberta.ca
Lewis
Michael J
MJ
Morrissey
Joshua A
JA
Flegel
Mitchel D
MD
Jeroncic
Kevin
K
Xiong
Yeping
Y
Cheng
Dean
D
Eisner
Roman
R
Gautam
Bijaya
B
Tzur
Dan
D
Sawhney
Summit
S
Bamforth
Fiona
F
Greiner
Russ
R
Li
Liang
L
D002556:Cerebrospinal Fluid Proteins / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D019295:Computational Biology / Q000379:methods*; D005583:Fourier Analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular
D002556:Cerebrospinal Fluid Proteins