Cytokine release and expression induced by OmpA proteins from the Gram-negative anaerobes, Porphyromonas asaccharolytica and Bacteroides fragilis.
OmpA proteins from Gram-negative anaerobes Porphyromonas asaccharolytica and Bacteroides fragilis induced release and expression of IL-1alpha, tumor necrosis factor (TNF)-alpha, IFN-gamma, IL-6, and IL-10 from murine splenocytes in vitro in a dose-dependent fashion. The release of the cytokines induced by B. fragilis Bf-OmpA was at much lower levels compared with P. asaccharolytica Omp-PA; Bf-OmpA did not induce release of IL-10. Omp-PA and Bf-OmpA were able to upregulate mRNA expression of the tested cytokines. The results obtained with refolded Bf-OmpA were similar to those with native Bf-OmpA. The data presented in this research demonstrate for the first time that Omps from anaerobic bacteria can induce the release of cytokines, suggesting that Omp-PA and Bf-OmpA may play important roles in the pathogenic processes of these bacteria.
Magalashvili
Lana
L
The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Lazarovich
Shirley
S
Pechatnikov
Izabella
I
Wexler
Hannah M
HM
Nitzan
Yeshayahu
Y
D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001441:Bacteroides fragilis / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004306:Dose-Response Relationship, Immunologic; D020869:Gene Expression Profiling; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D051379:Mice; D017861:Porphyromonas / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013154:Spleen / Q000276:immunology; D015854:Up-Regulation
D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines; D012333:RNA, Messenger; C078167:OMPA outer membrane proteins