title: Alteration of GyrA amino acid required for ciprofloxacin resistance in Klebsiella pneumoniae isolates in China.
abstract: Resistance to ciprofloxacin was detected in 111 (48.1%) isolates of Klebsiella pneumoniae from China. GyrA alterations were identified in the ciprofloxacin-resistant and ciprofloxacin-susceptible isolates. The results, including previously published data, indicate that the single substitution Ser83-->Ile and three types of double mutations at Ser83 and Asp87 were required for ciprofloxacin resistance (P < 0.05).
authors.affiliation: Department of Medical Microbiology, Harbin Medical University, Pathogenic Biology Key Laboratory of Heilongjiang Province, 194 Xue Fu Road, Harbin 150086, China.
authors.forename: Yingmei
authors.initials: Y
authors.lastname: Fu
authors.forename: Lishuang
authors.initials: L
authors.lastname: Guo
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Wenli
authors.initials: W
authors.lastname: Zhang
authors.forename: Jiaao
authors.initials: J
authors.lastname: Gu
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Xu
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Chen
authors.forename: Yuehui
authors.initials: Y
authors.lastname: Zhao
authors.forename: Jiayu
authors.initials: J
authors.lastname: Ma
authors.forename: Xinghan
authors.initials: X
authors.lastname: Liu
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhang
mesh_terms: D019943:Amino Acid Substitution; D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics* / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D015363:Quinolones / Q000494:pharmacology
chemical_list: D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D015363:Quinolones; D002939:Ciprofloxacin; D027081:DNA Gyrase