[Differential analysis of associated proteome in hepatic fibrosis tissue].
OBJECTIVE
To develop the 2-DE profiles of proteome from hepatic fibrosis tissue and preliminarily analyze the differential expression of proteome.


METHODS
The differential expression of proteins were analyzed by imaging analysis and MALDI-TOF-MS after the total protein was extracted from the hepatic fibrosis tissue and the normal liver tissue by 2-DE. 3 proteins were verified by in Western-blotting.


RESULTS
Fifty-nine differentially expressed protein were found in the proteome profile analysis of these two types of tissue, among which 30 protein spots were up-regulated and 29 protein spots were down-regulated in these hepatic fibrosis tissues. Fifteen protein spots with the differential expressions more than 3 times were analyzed by peptide mass fingerprint (PMF) and 10 differentially expressed proteins were found to be related with cell signal transduction, cell proliferation, and oxidative stress. Western-blotting results of 14-3-3beta, DJ-1, and PEBP were consistent with those by the 2-DE examination.


CONCLUSIONS
Some differentially expressed proteins have been found in the hepatic fibrosis tissue and the normal liver tissue. These data provided a fundamental basis for further study of the mechanism of hepatic fibrogenesis. Proteome;
Luo
Xin-hua
XH
Department of Infectious Diseases, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, China.
Yang
Qin
Q
Zhang
Quan
Q
Cheng
Ming-liang
ML
D000328:Adult; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D006801:Humans; D008103:Liver Cirrhosis / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome