title: Impact of anaerobic growth conditions on toxic shock syndrome toxin-i production by Staphylococcus aureus.
abstract: OBJECTIVE
  The impact of anaerobic growth conditions on the Staphylococcus aureus toxic shock syndrome toxin-1 (TSST-1) production was studied.
  
  
  METHODS
  Ten strains of S. aureus derived from patients with toxic shock syndrome (TSS), 10 isolates of S. aureus, and documented TSST-1-producing strains recovered from patients with either staphylococcal septicemia or staphylococcal nongenital abscesses were grown under aerobic and anaerobic conditions. The bacterial growth was measured using optical density (OD) determinations at 520 nm. The toxin production was assayed using the TS-RPLA latex agglutination test.
  
  
  RESULTS
  Both TSS and non-TSS strains of S. aureus grown under aerobic and anaerobic conditions exhibited comparable OD patterns of growth, and the levels of toxin production remained constant during the logarithmic phase. Toxin titers developed during the logarithmic growth phase and peaked after 24 h of incubation. When stationary-phase isolates grown initially under aerobic conditions were subjected to strict anaerobic conditions, subsequent toxin titers, compared with isolates grown in the continued presence of oxygen, were depressed 2-fold, peaking at a later time.
  
  
  CONCLUSIONS
  TSST-1 production is diminished under continued anaerobic conditions.
authors.affiliation: Department of Medical Microbiology Creighton University School of Medicine, Omaha, NE, USA.
authors.forename: M
authors.initials: M
authors.lastname: Lungren
authors.forename: P
authors.initials: P
authors.lastname: Chaisilwattana
authors.forename: F C
authors.initials: FC
authors.lastname: Knoop
authors.forename: G R
authors.initials: GR
authors.lastname: Monif