title: Kinetics of semicarbazide and nitrofurazone in chicken eggs and egg powders.
abstract: The accumulation, depletion and partitioning of semicarbazide (SEM) and its parent compound nitrofurazone (NFZ) in eggs were studied using hens fed NFZ at therapeutic and sub-therapeutic levels. Dietary NFZ correlated strongly with NFZ and total SEM in eggs, while 28% of observed SEM was present in the form of parent NFZ. Depletion half-life in eggs was 2.4 days for SEM and 1.1 days for NFZ. NFZ accumulated preferentially in yolk (57-63%) as opposed to albumen, while 71-80% of SEM was found in yolk. In whole egg, 29% of SEM was present as tissue-bound residues compared with 80% in breast muscle. Whilst NFZ and SEM were partly degraded by pasteurization and spray drying, sufficient NFZ remained to suggest it might be detectable in egg powders when SEM is observed at low microg kg(-1) concentrations. NFZ was detectable in whole eggs during ingestion of only 0.1% of the therapeutic NFZ dose, making detection of intact NFZ in eggs a feasible means to prove conclusively the administration of this banned compound.
authors.affiliation: School of Biological Sciences, Queen's University Belfast, Belfast, UK.
authors.forename: K M
authors.initials: KM
authors.lastname: Cooper
authors.forename: J
authors.initials: J
authors.lastname: Le
authors.forename: C
authors.initials: C
authors.lastname: Kane
authors.forename: D G
authors.initials: DG
authors.lastname: Kennedy
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000493:pharmacokinetics*; D002273:Carcinogens / Q000493:pharmacokinetics; D002645:Chickens / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods; D004350:Drug Residues / Q000032:analysis*; D004529:Egg White / Q000737:chemistry; D004530:Egg Yolk / Q000378:metabolism; D004531:Eggs / Q000032:analysis*; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D009583:Nitrofurazone / Q000493:pharmacokinetics*; D012663:Semicarbazides / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000890:Anti-Infective Agents; D002273:Carcinogens; D012663:Semicarbazides; C010059:carbamylhydrazine; D009583:Nitrofurazone