Unusual case of Epstein-Barr virus DNA tissue positive: blood negative in a patient with post-transplant lymphoproliferative disorder.
Detection of PTLD uses PCR to detect circulating EBV DNA in the blood or in situ hybridization to identify EBV DNA in tissue biopsies. EBV DNA was detected in the tissue section using both real-time PCR and in situ hybridization. We report an unusual presentation of PTLD with no detectable EBV DNA in the blood using EBER-1 and EBNA-1 PCR assays. This report suggests that the use of EBV-PCR for the early detection of PTLD in blood samples may not be 100% effective in detecting disease.
Levenson
Brian M
BM
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-8840, USA.
Ali
Sabina A
SA
Timmons
Charles F
CF
Mittal
Naveen
N
Muthukumar
Alagar
A
Payne
Deborah A
DA
D002648:Child; D004279:DNA, Viral / Q000032:analysis*; D005260:Female; D006084:Graft Rejection / Q000188:drug therapy; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D016756:Immunoglobulins, Intravenous / Q000008:administration & dosage; D016031:Liver Transplantation / Q000276:immunology*; D008214:Lymphocytes / Q000473:pathology; D008232:Lymphoproliferative Disorders / Q000097:blood / Q000378:metabolism*; D008775:Methylprednisolone / Q000008:administration & dosage; D016133:Polymerase Chain Reaction; D019562:Viral Load
D004279:DNA, Viral; D016756:Immunoglobulins, Intravenous; D008775:Methylprednisolone