title: HPMA copolymer-doxorubicin-gadolinium conjugates: synthesis, characterization, and in vitro evaluation.
abstract: This study describes the synthesis, characterization, and in vitro evaluation of N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-gadolinium (Gd)-doxorubicin (Dox) conjugates. Copolymers of HPMA were derivatized to incorporate side chains for Gd chelation and Dox conjugation. The conjugates were characterized by their side chain contents, T(1) relaxivity (r(1)), stability, and in vitro cytotoxicity. High stability and relaxivity of these conjugates coupled with low toxicity show their potential for monitoring the in vivo fate of HPMA-based drug delivery systems by magnetic resonance imaging techniques.
authors.affiliation: Department of Pharmaceutical Sciences, University of Maryland, Baltimore, Maryland 21201, USA.
authors.forename: Bahar
authors.initials: B
authors.lastname: Zarabi
authors.forename: Anjan
authors.initials: A
authors.lastname: Nan
authors.forename: Jiachen
authors.initials: J
authors.lastname: Zhuo
authors.forename: Rao
authors.initials: R
authors.lastname: Gullapalli
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Ghandehari
mesh_terms: D000818:Animals; D000903:Antibiotics, Antineoplastic / Q000737:chemistry* / Q000494:pharmacology; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000737:chemistry* / Q000494:pharmacology; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology; D005682:Gadolinium / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D051379:Mice; D041681:NIH 3T3 Cells; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000903:Antibiotics, Antineoplastic; D004337:Drug Carriers; D008689:Methacrylates; D011109:Polymethacrylic Acids; D004317:Doxorubicin; D005682:Gadolinium; C032802:hydroxypropyl methacrylate