The effects of pH on carbon material and energy balances in hydrogen-producing Clostridium tyrobutyricum JM1.
The effects of pH on hydrogen fermentation of glucose by newly isolated H(2)-producing bacterium Clostridium tyrobutyricum JM1 were investigated in batch cultivations. The changes of carbon material and energy balances by pH conditions provided useful information for understanding and interpreting the regulatory system of the microorganism, and for optimization of a desired product, in this case, molecular hydrogen. The most probable metabolic pathways of C. tyrobutyricum JM1 were determined through an accurate analysis of stoichiometry and the consistency of the experimental data, checked by high carbon recovery. The carbon material and energy balances were adequately applied to estimate the carbon-flow distribution. They suggested that pH 6.3 was appropriate to maximize hydrogen production with a high concentration of butyrate and balanced activities of NADH.
Jo
Ji Hye
JH
Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Nam-gu, Pohang, Gyeongbuk 790-784, South Korea.
Lee
Dae Sung
DS
Park
Jong Moon
JM
D000693:Anaerobiosis; D002244:Carbon / Q000378:metabolism*; D048014:Clostridium tyrobutyricum / Q000378:metabolism*; D004734:Energy Metabolism; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration
D002244:Carbon; D006859:Hydrogen; D005947:Glucose