B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells.
Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN(-)B220(-)CD19(-)CD127(+)FLT3(+)SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19.
Mansson
Robert
R
Department for Biomedicine and Surgery, Linköping University, Linköping, Sweden.
Zandi
Sasan
S
Anderson
Kristina
K
Martensson
Inga-Lill
IL
Jacobsen
Sten Eirik W
SE
Bryder
David
D
Sigvardsson
Mikael
M
D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis*; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology*; D001854:Bone Marrow Cells; D019070:Cell Lineage; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit; D017493:Leukocyte Common Antigens / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
D018941:Antigens, CD19; D053645:Interleukin-2 Receptor alpha Subunit; D017493:Leukocyte Common Antigens