Identification of efficient and sequence specific bimolecular artificial ribonucleases by a combinatorial approach.
Chemically modified nucleotide monomers were incorporated into adjacent terminal positions of two separate oligonucleotides complementary to an RNA target; all possible combinations of the catalytic units were tested, resulting in an artificial nuclease that showed high activity and catalytic turnover.
Kalek
Marcin
M
The Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, DK-5230, Odense M, Denmark.
Benediktson
Peter
P
Vester
Birte
B
Wengel
Jesper
J
D001483:Base Sequence; D020650:Combinatorial Chemistry Techniques; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D015195:Drug Design; D008969:Molecular Sequence Data; D012260:Ribonucleases / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013379:Substrate Specificity
D004247:DNA; D012260:Ribonucleases