title: Aqueous suspension of carbon nanotubes enhances the specificity of long PCR.
abstract: DNA manipulation technology is facing more challenges in the postgenomics era. More and more nanomaterials have been investigated for their potential implications in developing better gene technology. In this study, we reported the beneficial effect of single-walled carbon nanotubes (SWCNTs) and multi-walled carbon nanotubes (MWCNTs) in enhancing the specificity and total efficiency of long (14 kb) PCR. Hydroxylic and carboxylic carbon nanotubes (CNTs) had similar enhancing effects. Nanotubes could become another component for improvements in the amplification of long DNA.
authors.affiliation: Teda Bio-X Center for Systems BioTechnology, Tianjin University of Science and Technology, Tianjin, China. zhangzzbiox@gmail.com
authors.forename: Zhizhou
authors.initials: Z
authors.lastname: Zhang
authors.forename: Cencao
authors.initials: C
authors.lastname: Shen
authors.forename: Mingchun
authors.initials: M
authors.lastname: Wang
authors.forename: Han
authors.initials: H
authors.lastname: Han
authors.forename: Xiaohong
authors.initials: X
authors.lastname: Cao
mesh_terms: D010582:Bacteriophage lambda / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D016133:Polymerase Chain Reaction / Q000379:methods*; D011208:Powders; D012680:Sensitivity and Specificity; D017422:Sequence Analysis, DNA; D013698:Templates, Genetic; D014199:Trehalose / Q000737:chemistry; D014764:Viral Proteins; D014867:Water / Q000737:chemistry
chemical_list: D004279:DNA, Viral; D037742:Nanotubes, Carbon; D011208:Powders; D014764:Viral Proteins; D014867:Water; C071648:gene 9 protein, Rotavirus B; D014199:Trehalose; D004259:DNA-Directed DNA Polymerase