title: Mitochondria, endoplasmic reticulum and actin filament behavior after PDT with chloroaluminum phthalocyanine liposomal in HeLa cells.
abstract: Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotoxicity in specific cellular targets of a photosensitizer used in PDT in vitro. The photosensitizing effects of chloroaluminum phthalocyanine liposomal were studied on the mitochondria, cytoskeleton and endoplasmic reticulum of HeLa cells. Cells were irradiated with a diode laser working at 670 nm, energy density of 4.5 J/cm2 and power density of 45 mW/cm2. Fluorescence microscopic analysis of the mitochondria showed changes in membrane potential. After PDT treatment, the cytoskeleton and endoplasmic reticulum presented basic alterations in distribution. The combined effect of AlPHCl liposomal and red light in the HeLa cell line induced photodamage to the mitochondria, endoplasmic reticulum and actin filaments in the cytoskeleton.
authors.affiliation: Laboratory of Cellular Culture and Tecidual Biology, Dynamics of Cellular Compartments, Universidade do Vale do Paraíba, UNIVAP, Av. Shishima Hifumi 2911, 12211-300 São José dos Campos, SP, Brazil.
authors.forename: Maíra
authors.initials: M
authors.lastname: Maftoum-Costa
authors.forename: Karina Teixeira
authors.initials: KT
authors.lastname: Naves
authors.forename: Alexandre Lima
authors.initials: AL
authors.lastname: Oliveira
authors.forename: Antonio Cláudio
authors.initials: AC
authors.lastname: Tedesco
authors.forename: Newton Soares
authors.initials: NS
authors.lastname: da Silva
authors.forename: Cristina
authors.initials: C
authors.lastname: Pacheco-Soares
mesh_terms: D008841:Actin Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D045744:Cell Line, Tumor; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000648:ultrastructure; D006367:HeLa Cells; D006801:Humans; D007211:Indoles / Q000494:pharmacology*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D008928:Mitochondria / Q000187:drug effects*; D009942:Organometallic Compounds / Q000494:pharmacology*; D010778:Photochemotherapy; D011838:Radiation-Sensitizing Agents / Q000494:pharmacology
chemical_list: D007211:Indoles; D009942:Organometallic Compounds; D011838:Radiation-Sensitizing Agents; C044797:chloroaluminum phthalocyanine