Transient up-regulation of a novel member of Spot 14 family in androgen-stimulated rat prostate.
The rat prostate is dependent on androgen for growth and differentiation. In an effort to find novel genes involved in androgen-induced growth of the rat prostate, we carried out PCR-based subtractive hybridization and identified several factors that were transiently up-regulated after androgen stimulation in castrated rat prostate. Among them, a novel member of the Spot 14 family has been identified. This protein (Spot 14-like androgen-inducible protein, SLAP) exhibited the highest homology (about 50%) with zebrafish gastrulation-specific G12 protein and about 30% homology with rat Spot 14. The SLAP mRNA level decreased following castration and transiently increased after testosterone replacement, attaining a peak 3 days after the treatment. The change in the protein level was similar to that in mRNA except that it was low in both untreated and castrated rat prostate tissue. In normal adult rats, SLAP was expressed at relatively high levels in the lung, stomach and liver. Unlike the prostate, SLAP expression in the lung was not affected by the androgen status. Like other members of the Spot 14 family, SLAP has a leucine-zipper motif in its C-terminal region, making it possible to form a stable homodimer. Though the physiological role of SLAP remains to be clarified, the current results suggest that SLAP expression is associated with some growth-related processes in regrowing rat prostate.
Nishi
Nozomu
N
Department of Endocrinology, Faculty of Medicine, Kagawa University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0793, Japan. nnishi@med.kagawa-u.ac.jp
Shoji
Hiroki
H
Miyanaka
Hiroshi
H
Nakamura
Takanori
T
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000728:Androgens / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics; D019281:Dimerization; D007279:Injections, Subcutaneous; D008297:Male; D008969:Molecular Sequence Data; D008970:Molecular Weight; D009154:Mutation; D009919:Orchiectomy; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000737:chemistry / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017386:Sequence Homology, Amino Acid; D043343:Testosterone Propionate / Q000008:administration & dosage / Q000494:pharmacology; D013997:Time Factors; D015854:Up-Regulation / Q000187:drug effects*
D048868:Adaptor Proteins, Signal Transducing; D000728:Androgens; D018076:DNA, Complementary; D012333:RNA, Messenger; C490636:Sla protein, rat; D016392:Proto-Oncogene Proteins pp60(c-src); D043343:Testosterone Propionate