Detection of a high affinity binding site in recombinant Aleuria aurantia lectin.
Lectins are carbohydrate binding proteins that are involved in many recognition events at molecular and cellular levels. Lectin-oligosaccharide interactions are generally considered to be of weak affinity, however some mushroom lectins have unusually high binding affinity towards oligosaccharides with K (d) values in the micromolar range. This would make mushroom lectins ideal candidates to study protein-carbohydrate interactions. In the present study we investigated the properties of a recombinant form of the mushroom lectin Aleuria aurantia (AAL). AAL is a fucose-binding lectin composed of two identical 312-amino acid subunits. Each subunit contains five binding sites for fucose. We found that one of the binding sites in rAAL had unusually high affinities towards fucose and fucose-containing oligosaccharides with K (d) values in the nanomolar range. This site could bind to oligosaccharides with fucose linked alpha1-2, alpha1-3 or alpha1-4, but in contrast to the other binding sites in AAL it could not bind oligosaccharides with alpha1-6 linked fucose. This binding site is not detected in native AAL (nAAL) one possible explanation may be that this site is blocked with free fucose in nAAL. Recombinant AAL was produced in E. coli as a His-tagged protein, and purified in a one-step procedure. The resulting protein was analyzed by electrophoresis, enzyme-linked lectin assay and circular dichroism spectroscopy, and compared to nAAL. Binding properties were measured using tryptophan fluorescence and surface plasmon resonance. Removal of the His-tag did not alter the binding properties of recombinant AAL in the enzyme-linked lectin assay. Our study forms a basis for understanding the AAL-oligosaccharide interaction and for using molecular techniques to design lectins with novel specificities and high binding affinities towards oligosaccharides.
Olausson
Johan
J
Department of Clinical and Experimental Medicine, Division of Cell Biology, Linköping University, SE-581 85, Linköping, Sweden. johol@ibk.liu.se
Tibell
Lena
L
Jonsson
Bengt-Harald
BH
Påhlsson
Peter
P
D001203:Ascomycota / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D002240:Carbohydrate Sequence; D005643:Fucose / Q000737:chemistry / Q000378:metabolism; D006386:Hemagglutination Tests; D006801:Humans; D066298:In Vitro Techniques; D007700:Kinetics; D037102:Lectins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry / Q000378:metabolism; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D020349:Surface Plasmon Resonance; D014364:Tryptophan / Q000737:chemistry
D037102:Lectins; D009844:Oligosaccharides; D011994:Recombinant Proteins; C064549:lectin, Aleuria aurantia; D005643:Fucose; D014364:Tryptophan