title: Differential expression of CD150 (SLAM) family receptors by human hematopoietic stem and progenitor cells.
abstract: OBJECTIVE
  Human hematopoietic stem cell (HSC)-containing grafts are most commonly used to treat various blood diseases, including leukemias and autoimmune disorders. CD150 (SLAM) family receptors have recently been shown to be differentially expressed by mouse HSC and progenitor cells. Members of the CD150 family are key regulators of leukocyte activation and differentiation. The goal of the present study is to analyze the expression patterns of the CD150 receptors CD48, CD84, CD150 (SLAM), CD229 (Ly9), and CD244 (2B4) on the different sources of human hematopoietic stem and progenitor cells.
  
  
  METHODS
  Expression of CD150 receptors was analyzed on human mobilized peripheral blood CD133(+)-isolated cells and CD34(+) bone marrow (BM) and umbilical cord blood (CB) cells using multicolor flow cytometry.
  
  
  RESULTS
  CD244 was present on most CD133(+)Lin(-)-mobilized cells and CD34(+)Lin(-) BM and CB cells, including virtually all CD38(-)Lin(-) primitive progenitor cells. CD48 had a restricted expression pattern on CD133(+)Lin(-)CD38(-) cells, while its levels were significantly higher in CD34(+)Lin(-) BM and CB cells. In addition, CD84 was present on a significant number of CD133(+)Lin(-) cells, but only on a small fraction of CD133(+)Lin(-)CD38(-) peripheral blood mobilized cells. In contrast, CD84 was expressed on practically all CD34(+)Lin(-) BM cells. No CD150 expression was observed in mobilized peripheral blood CD133(+)Lin(-) or CD34(+)Lin(-) BM and CB cells. Furthermore, only a small fraction of CD34(+)Lin(-) BM and CB cells expressed CD229.
  
  
  CONCLUSIONS
  Our results show that CD150 family molecules are present on human hematopoietic stem and progenitor cells and that their expression patterns differ between humans and mice.
authors.affiliation: Department of Cellular Biology and Pathology, Medical School, University of Barcelona, Barcelona, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Sintes
authors.forename: Xavier
authors.initials: X
authors.lastname: Romero
authors.forename: Pedro
authors.initials: P
authors.lastname: Marin
authors.forename: Cox
authors.initials: C
authors.lastname: Terhorst
authors.forename: Pablo
authors.initials: P
authors.lastname: Engel
mesh_terms: D000328:Adult; D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis* / Q000235:genetics; D000071178:CD48 Antigen; D002478:Cells, Cultured / Q000378:metabolism; D005312:Fetal Blood / Q000166:cytology; D005786:Gene Expression Regulation; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D007231:Infant, Newborn; D051379:Mice; D005810:Multigene Family / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000096:biosynthesis* / Q000235:genetics; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics; D000071176:Signaling Lymphocytic Activation Molecule Family; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1; D013045:Species Specificity
chemical_list: D015703:Antigens, CD; C084093:CD244 protein, human; D000071178:CD48 Antigen; C000604065:CD48 protein, human; C498970:CD84 protein, human; C000604066:Cd48 protein, mouse; C430423:LY9 protein, human; D011956:Receptors, Cell Surface; D011971:Receptors, Immunologic; C000604059:SLAMF1 protein, human; D000071176:Signaling Lymphocytic Activation Molecule Family; D000071177:Signaling Lymphocytic Activation Molecule Family Member 1