title: [Evaluation of gene index for identification of Byssochlamys spp].
abstract: Comparative sequence analysis was performed on the 18S rRNA gene (1,676 bp), 26/28S rRNA gene D2 region (321 bp) and lys2 (997 bp) to evaluate the gene index for rapid, accurate and convenient identification of Byssochlamys spp. and related species. The results showed that 26 strains (11 species) of the clade could be identified or grouped by means of each gene sequence. The highest resolution to discriminate these species was observed with lys2, but 26/28S rRNA gene D2 region was considered to be the best index for convenient identification. In addition, phylogenetic analysis based on these genes indicated that genus Byssochlamys is not monophyletic, although species in the clade are closely related to each other. Re-classification will be necessary, based on detailed morphological observations.
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan.
authors.forename: Maiko
authors.initials: M
authors.lastname: Watanabe
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kato
authors.forename: Keiko
authors.initials: K
authors.lastname: Togami
authors.forename: Mikiko
authors.initials: M
authors.lastname: Yamanaka
authors.forename: Keiko
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ogawa
authors.forename: Yuko
authors.initials: Y
authors.lastname: Wasada
authors.forename: Keiichi
authors.initials: K
authors.lastname: Goto
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Hara-Kudo
authors.forename: Norihide
authors.initials: N
authors.lastname: Amano
authors.forename: Akira
authors.initials: A
authors.lastname: Yokota
mesh_terms: D015074:2-Aminoadipic Acid / Q000235:genetics; D001203:Ascomycota / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D012331:RNA, Fungal / Q000235:genetics; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D012339:RNA, Ribosomal, 28S / Q000235:genetics; D017422:Sequence Analysis, DNA; D017423:Sequence Analysis, RNA
chemical_list: D012331:RNA, Fungal; D012337:RNA, Ribosomal, 18S; D012339:RNA, Ribosomal, 28S; D015074:2-Aminoadipic Acid