title: Determination of total sterols in brown algae by Fourier transform infrared spectroscopy.
abstract: A procedure is proposed for the determination of the total amount of sterols in brown algae Bifurcaria bifurcata, Cladostephus hirsitus, Dictyota dichotoma and Cystoseira sedoides, globally determined as fucosterol, which is the major sterol contained in these algae. The method involves the use of cholesterol as reference standard and a correction factor of 1.259+/-0.003, which represents the ratio between the slopes of calibration lines obtained from fucosterol and cholesterol. The method provides precise and accurate results for the IR analysis of real samples.
authors.affiliation: Laboratory of Medicinal and Aromatic Plants, University of Blida, P.O.B. 270 Soumâa (Blida 09000), Algeria.
authors.forename: Naïma
authors.initials: N
authors.lastname: Bouzidi
authors.forename: Yasmina
authors.initials: Y
authors.lastname: Daghbouche
authors.forename: Mohamed
authors.initials: M
authors.lastname: El Hattab
authors.forename: Zahia
authors.initials: Z
authors.lastname: Aliche
authors.forename: Gérald
authors.initials: G
authors.lastname: Culioli
authors.forename: Louis
authors.initials: L
authors.lastname: Piovetti
authors.forename: Salvador
authors.initials: S
authors.lastname: Garrigues
authors.forename: Miguel
authors.initials: M
authors.lastname: de la Guardia
mesh_terms: D000459:Phaeophyceae / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods; D013261:Sterols / Q000032:analysis*
chemical_list: D013261:Sterols