title: [Differentially expressed genes between upward and downward progressing types of nasopharyngeal carcinoma].
abstract: OBJECTIVE
  Upward (local growth and invasion of the base of skull), downward (distant metastasis) and mixed progressing types of nasopharygeal carcinoma (NPC) have been observed when the disease progress to middle-late stage. The upward and downward progressing types are evidently different in clinical symptom, therapy strategy and prognosis. Identification of the molecular differences between them is very important for molecular classification, prognostic prediction and research on neoplasia and development of NPC. This study was to discover the genes differentially expressed in upward and downward progressing types of NPC.
  
  
  METHODS
  An oligo gene chip containing 21 300 genes was used to detect the genes differentially expressed between upward and downward progressing types of NPC. One of the differentially expressed genes detected was analyzed by reverse transcription-polymerase chain reaction (RT-PCR).
  
  
  RESULTS
  Seventeen genes were differentially expressed between upward and downward progressing types of NPC. The expression of SELB, Clorf29, GLE1L and FLJ20989 genes were up-regulated and the expression of 1D12A, ASPN, DCN, PRO2219, LRDD, DIO2, ULBP2, PRO3073, IGVH3, IGVH4, IGLJ3, PRO0943 and AK057247 genes were down-regulated in the upward progressing type as compared with those in the downward progressing type. The difference of gene expression was ranged from 2.30 to 4.23 folds. The high expression rate of DIO2 gene was significantly higher in downward progressing type than in upward progressing type (90.0% vs. 33.3%, P = 0.020).
  
  
  CONCLUSIONS
  The gene expression patterns are significantly different between upward and downward progressing types of NPC. The expression of DIO2 gene is higher in the downward progressing type than in upward progressing type, which may be closely related to the metastasis potential of NPC.
authors.affiliation: State Key Laboratory of Oncology, Guangzhou, Guangdong, 510060, People's Republic of China.
authors.forename: Wei-Jiang
authors.initials: WJ
authors.lastname: Liang
authors.forename: Fang
authors.initials: F
authors.lastname: Qiu
authors.forename: Ming-Huang
authors.initials: MH
authors.lastname: Hong
authors.forename: Ling
authors.initials: L
authors.lastname: Guo
authors.forename: Hai-De
authors.initials: HD
authors.lastname: Qin
authors.forename: Qi-Cai
authors.initials: QC
authors.lastname: Liu
authors.forename: Xiao-Shi
authors.initials: XS
authors.lastname: Zhang
authors.forename: Hai-Qiang
authors.initials: HQ
authors.lastname: Mai
authors.forename: Yan-Qun
authors.initials: YQ
authors.lastname: Xiang
authors.forename: Hua-Qing
authors.initials: HQ
authors.lastname: Min
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Zeng
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000145:classification / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007453:Iodide Peroxidase / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000145:classification / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D009362:Neoplasm Metastasis; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019292:Skull Base Neoplasms / Q000235:genetics / Q000378:metabolism / Q000556:secondary; D000097564:Iodothyronine Deiodinase Type II
chemical_list: D007453:Iodide Peroxidase; D000097564:Iodothyronine Deiodinase Type II