Superiority of bone marrow-derived dendritic cells over monocyte-derived ones for the expansion of regulatory T cells in the macaque.
Regulatory T cells (Treg) have been identified as playing a pivotal role in the control of tolerance and in the suppression of pathologic immune responses in autoimmune diseases, transplantation, and graft-versus-host disease. Treg expanded ex vivo by dendritic cells could be potential reagents to promote antigen-specific tolerance in vivo. However, in vivo studies have been carried out mostly in rodents and will need validation in primates before clinical application. We characterized macaque dendritic cell derived either from bone marrow with and without prior CD34+ cell selection (BMDC), or from CD14+ peripheral blood mononuclear cells (Mo-DC). We demonstrate that with a semi-mature phenotype, BMDC are superior to Mo-DC in their capacity to expand freshly isolated allogeneic macaque CD4+ CD25+ CD127- Foxp3+ Treg in vitro in the presence of interleukin-2. Moreover, the expanded Treg maintain their phenotype and suppressive activity. These data provide a step toward the use of macaque dendritic cell to expand Treg for future preclinical testing.
Moreau
Aurélie
A
INSERM U649, CHU Hôtel-dieu, Nantes Cedex, France.
Chiffoleau
Elise
E
Beriou
Gaëlle
G
Deschamps
Jack-Yves
JY
Heslan
Michèle
M
Ashton-Chess
Joanna
J
Rolling
Fabienne
F
Josien
Regis
R
Moullier
Philippe
P
Cuturi
Maria-Cristina
MC
Alliot-Licht
Brigitte
B
D000818:Animals; D018952:Antigens, CD34 / Q000276:immunology; D001854:Bone Marrow Cells / Q000166:cytology* / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D016130:Immunophenotyping; D053645:Interleukin-2 Receptor alpha Subunit / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008213:Lymphocyte Activation; D008252:Macaca fascicularis; D023421:Models, Animal; D009000:Monocytes / Q000166:cytology* / Q000276:immunology*; D050378:T-Lymphocytes, Regulatory / Q000276:immunology*
D018952:Antigens, CD34; D053645:Interleukin-2 Receptor alpha Subunit; D018950:Lipopolysaccharide Receptors