Excitation dynamics of two spectral forms of the core complexes from photosynthetic bacterium Thermochromatium tepidum.
The intact core antenna-reaction center (LH1-RC) core complex of thermophilic photosynthetic bacterium Thermochromatium (Tch.) tepidum is peculiar in its long-wavelength LH1-Q(y) absorption (915 nm). We have attempted comparative studies on the excitation dynamics of bacteriochlorophyll (BChl) and carotenoid (Car) between the intact core complex and the EDTA-treated one with the Q(y) absorption at 889 nm. For both spectral forms, the overall Car-to-BChl excitation energy transfer efficiency is determined to be approximately 20%, which is considerably lower than the reported values, e.g., approximately 35%, for other photosynthetic purple bacteria containing the same kind of Car (spirilloxanthin). The RC trapping time constants are found to be 50 approximately 60 ps (170 approximately 200 ps) for RC in open (closed) state irrespective to the spectral forms and the wavelengths of Q(y) excitation. Despite the low-energy LH1-Q(y) absorption, the RC trapping time are comparable to those reported for other photosynthetic bacteria with normal LH1-Q(y) absorption at 880 nm. Selective excitation to Car results in distinct differences in the Q(y)-bleaching dynamics between the two different spectral forms. This, together with the Car band-shift signals in response to Q(y) excitation, reveals the presence of two major groups of BChls in the LH1 of Tch. tepidum with a spectral heterogeneity of approximately 240 cm(-1), as well as an alteration in BChl-Car geometry in the 889-nm preparation with respect to the native one.
Ma
Fei
F
Beijing National Laboratory for Molecular Science, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, China.
Kimura
Yukihiro
Y
Zhao
Xiao-Hui
XH
Wu
Yi-Shi
YS
Wang
Peng
P
Fu
Li-Min
LM
Wang
Zheng-Yu
ZY
Zhang
Jian-Ping
JP
D001426:Bacterial Proteins / Q000378:metabolism*; D001429:Bacteriochlorophylls / Q000378:metabolism; D002338:Carotenoids / Q000378:metabolism; D002841:Chromatiaceae / Q000737:chemistry*; D004735:Energy Transfer; D017785:Photons; D045322:Photosynthetic Reaction Center Complex Proteins / Q000378:metabolism*; D013057:Spectrum Analysis
D001426:Bacterial Proteins; D001429:Bacteriochlorophylls; D045322:Photosynthetic Reaction Center Complex Proteins; D002338:Carotenoids