NMR and MALDI-TOFMS analysis of a heteroglycan isolated from hot water extract of edible mushroom, Volvariella bombycina.
A water-soluble polysaccharide, isolated from the hot aqueous extract of an edible mushroom, Volvariella bombycina, consists of d-glucose, d-mannose, and d-galactose in a molar ratio 2:1:1. On the basis of total hydrolysis, methylation analysis, periodate oxidation, and NMR studies ((1)H, (13)C, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC), and MALDI-TOFMS analysis, the repeating unit of the polysaccharide is established as [table in text].
Das
Debsankar
D
Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
Maiti
Debabrata
D
Chandra
Krishnendu
K
Mondal
Subhas
S
Ojha
Arnab K
AK
Roy
Sadhan K
SK
Ghosh
Kaushik
K
Islam
Syed S
SS
D000363:Agaricales / Q000737:chemistry* / Q000378:metabolism; D002240:Carbohydrate Sequence; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008745:Methylation; D008956:Models, Chemical; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D010504:Periodic Acid / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D013696:Temperature; D014867:Water / Q000737:chemistry
D009005:Monosaccharides; D011134:Polysaccharides; D014867:Water; D010504:Periodic Acid; C009288:metaperiodate; D010100:Oxygen