title: A new purple fluorescent color marker for genetic studies in Saccharomyces cerevisiae and Candida albicans.
abstract: The ability to visualize cellular events by linking them to color or fluorescence changes has been an invaluable tool for biology. We describe a novel plasmid-borne color marker whose expression in yeast leads to purple-colored cells that are also brightly fluorescent. This dominant marker provides a useful tool for rapidly screening plasmid maintenance using a visual or fluorescence assay in both Saccharomyces cerevisiae and Candida albicans.
authors.affiliation: Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, USA.
authors.forename: Sabine
authors.initials: S
authors.lastname: Keppler-Ross
authors.forename: Christine
authors.initials: C
authors.lastname: Noffz
authors.forename: Neta
authors.initials: N
authors.lastname: Dean
mesh_terms: D001483:Base Sequence; D002176:Candida albicans / Q000166:cytology*; D005455:Fluorescent Antibody Technique; D005456:Fluorescent Dyes; D006651:Histocytochemistry / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D017422:Sequence Analysis, DNA; D000097573:Red Fluorescent Protein
chemical_list: D005456:Fluorescent Dyes; D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein