[Effect of interferon-gamma on the expression of vascular endothelial growth factor C on Hep-2 laryngeal carcinoma cell lines].
OBJECTIVE
To observe the effect of interferon-gamma on the expression of vascular endothelial growth factor C on Hep-2 laryngeal carcinoma cell lines.


METHODS
Hep-2 cell was exposed to interferon-gamma at a concentration (10(3), 10(4), 10(5), 10(6), 10(7) U/L) for 0, 12, 24, 36, 48, 60, 72 h hours. MTT colorimetric assay was used to evaluate the effect of interferon-gamma on Hep-2 cell proliferation after incubation with interferon-gamma. Hep-2 cell was harvested to be detected VEGF-C mRNA levels by realtime polymerase chain reaction (Realtime-PCR) with interferon-gamma (10(6) U/L) for 0, 2, 6, 12, 24, 36, 48, 60, 72 h hours. The VEGF-C protein level in supernatants was determined by enzyme-linked immunosorbent assay (ELISA) and in cytoplasma by immunocytochemical staining and Western blot analysis with the same drug treatment as Realtime-PCR.


RESULTS
Hep-2 cell was significantly inhibited by IFN-gamma at high concentration. Expression of VEGF-C mRNA and protein levels of Hep-2 cell at 10(6) U/L concentrations of interferon-gamma were lower than that of normal control (P < 0.05) at 48 h.


CONCLUSIONS
IFN-gamma can inhibit the expression of VEGF-C in Hep-2 cell with the level of mRNA and protein.
Chen
Jie
J
Department of Otolaryngology-Head and Neck Surgery, Renji Hospital, Shanghai Jiaotong University, Shanghai, 200001, China.
Zhou
Jiaqing
J
Wang
Jiadong
J
D045744:Cell Line, Tumor; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D007822:Laryngeal Neoplasms / Q000378:metabolism* / Q000473:pathology; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism*
C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C; D007371:Interferon-gamma