title: Antitumor Trans Platinum Adducts of GMP and AMP.
abstract: Recently it has been shown that several analogues of the clinically ineffective trans-DDP exhibit antitumor activity comparable to that of cis-DDP. The present paper describes the binding of antitumor trans-[PtCl(2)(E-iminoether)(2)] (trans-EE) to guanosinemonophosphate (GMP) and adenosinemonophosphate (AMP). We have used HPLC and (1)H and (15)N NMR to characterize the different adducts. In the case of a 1:1 mixture of trans-EE and GMP, at an early stage of the reaction, a monofunctional adduct is formed which, subsequently, is partly converted into a monosolvated monofunctional species. After about 70 hours an equilibrium is established between chloro and solvato monofunctional adducts at a ratio of 30/70. In the presence of excess GMP (4:1) the initially formed monofunctional adducts react further to give two bifunctional adducts, one with the iminoether ligands in their original E configurations and the other with the iminoether ligands having one E and the other, Z configurations. The coordination geometry obtained by energy minimization calculations is in qualitative agreement with 2D NMR data.
authors.affiliation: Department of Chemistry University of Bergen Allegt.41 Bergen N-5007 Norway.
authors.forename: Y
authors.initials: Y
authors.lastname: Liu
authors.forename: M F
authors.initials: MF
authors.lastname: Sivo
authors.forename: G
authors.initials: G
authors.lastname: Natile
authors.forename: E
authors.initials: E
authors.lastname: Sletten