Determination of illicit and medicinal drugs and their metabolites in oral fluid and preserved oral fluid by liquid chromatography-tandem mass spectrometry.
An LC-MS/MS method using 0.5 ml of oral fluid was developed for the determination of morphine, codeine, 6-monoacetylmorphine, methadone, amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine, 3,4-methylenedioxymethamphetamine, 3,4-methylenedioxy-N-ethylamphetamine, benzoylecgonine, cocaine, delta-9-tetrahydrocannabinol, zolpidem, zopiclone, alprazolam, clonazepam, oxazepam, nordiazepam, lorazepam, flunitrazepam, diazepam, diphenhydramine and amitriptyline. The method was fully validated in terms of linearity (the method was linear between 1-5 microg/L and 100-200 microg/L) recoveries (7.5-82.6%), within-day and between-day precisions and accuracies (CV and MRE, both <15%), limits of detection (0.5 microg/L) and quantitation (the lowest point on the calibration curve), relative ion intensities, freeze-and-thaw stability and matrix effect. The method was applied to preserved oral fluid collected by a special commercial device, the StatSure Saliva Sampler.
Concheiro
Marta
M
Forensic Toxicology Service. Institute of Legal Medicine, University of Santiago de Compostela Spain, C/ San Francisco s/n, 15782, Santiago de Compostela, Spain.
de Castro
Ana
A
Quintela
Oscar
O
Cruz
Angelines
A
López-Rivadulla
Manuel
M
D002853:Chromatography, Liquid; D006801:Humans; D013287:Illicit Drugs / Q000032:analysis*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D012463:Saliva / Q000737:chemistry*; D015813:Substance Abuse Detection / Q000379:methods*; D053719:Tandem Mass Spectrometry
D013287:Illicit Drugs; D004364:Pharmaceutical Preparations