title: Candida albicans HSP12 is co-regulated by physiological CO2 and pH.
abstract: Global transcriptional analysis of Candida albicans exposed to elevated ambient CO(2) revealed a statistically significant differential regulation of 14 genes. Subsequent RNA hybridisation analysis of one gene, HSP12, confirmed CO(2)-regulation via a cAMP-dependent mechanism. Additionally, Northern analyses and gel mobility shift assays demonstrate the co-regulation of HSP12 by environmental pH via a Rim101-dependent mechanism.
authors.affiliation: Biomedical Science Group, Department of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK.
authors.forename: Chirag C
authors.initials: CC
authors.lastname: Sheth
authors.forename: Estelle G
authors.initials: EG
authors.lastname: Mogensen
authors.forename: Man Shun
authors.initials: MS
authors.lastname: Fu
authors.forename: Ian C
authors.initials: IC
authors.lastname: Blomfield
authors.forename: Fritz A
authors.initials: FA
authors.lastname: Mühlschlegel
mesh_terms: D002176:Candida albicans / Q000235:genetics* / Q000378:metabolism; D002245:Carbon Dioxide / Q000378:metabolism*; D000242:Cyclic AMP / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic; D011994:Recombinant Proteins / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics
chemical_list: D004268:DNA-Binding Proteins; D005656:Fungal Proteins; C112591:HSP12 protein, S cerevisiae; D006360:Heat-Shock Proteins; C489602:RIM101 protein, Candida albicans; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D002245:Carbon Dioxide; D000242:Cyclic AMP