title: [Effects of vitamin C combined with arsenic trioxide on the apoptosis of Hep-2 cell].
abstract: OBJECTIVE
  To study synergistic effect of Vitamin C (VitC) combined with arsenic trioxide (As2 O3)-on human laryngocarcinoma Hep-2 cell line apoptosis.
  
  
  METHODS
  Human laryngocarcinoma cell line Hep-2 was cultured in vitro, and incubated with As2 O3 or jointly with VitC. The inhibition ability of Hep-2 cells was determined by methyl thiazolyl tetrazolium (MTT) assay, while the apoptosis was mensurated by the flow cytometry with Annexin-V/PI.
  
  
  RESULTS
  Compared with the use of As2 O3 separately, the combination of As2 O3 and VitC could increase the inhibition ability and the apoptosis rate of Hep-2 cells markedly (P < 0.05).
  
  
  CONCLUSIONS
  Combination of As2 O3 with VitC could markedly increase arsenic trioxide (As2 O3)-induced apoptosis of Hep-2 cells.
authors.affiliation: Department of Otolaryngology, Renmin Hospital of Tongshan County, 437600, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Xu
authors.forename: Chenghua
authors.initials: C
authors.lastname: Shu
authors.forename: Jin
authors.initials: J
authors.lastname: Hu
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Ye
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004357:Drug Synergism; D006801:Humans; D010087:Oxides / Q000494:pharmacology*
chemical_list: D001152:Arsenicals; D010087:Oxides; D001205:Ascorbic Acid; D000077237:Arsenic Trioxide