[Restriction endonuclease Asi256I recognizing the nucleotide sequence 5'-GATC-3'].
A strain producing a restriction endonuclease was isolated from soil samples and identified as the Arthrobacter sp. strain Ck256. The enzyme produced by this strain was termed Asi2561. The isolation procedure for this enzyme was described, and the optimal conditions for its function were determined. It was shown that the restriction endonuclease Asi256I is a true isoschizomer of MboI, it has a temperature optimum of 6 degrees C, and can be used in molecular-biological and genetic-engineering studies performed at low temperatures.
Ushakova
T A
TA
Puchkova
L I
LI
Gutorov
V V
VV
Totmenina
O D
OD
Repin
V E
VE
D001173:Arthrobacter / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification; D010582:Bacteriophage lambda / Q000235:genetics; D003080:Cold Temperature; D004262:DNA Restriction Enzymes / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism*; D004279:DNA, Viral / Q000235:genetics / Q000378:metabolism; D012988:Soil Microbiology; D013379:Substrate Specificity
D004279:DNA, Viral; D004262:DNA Restriction Enzymes