title: Effects of amino acids on microcystin production of the Microcystis aeruginosa.
abstract: A Microcystis aeruginosa which produced high content of microcystin-LR (MC-LR) but no microcystin-RR (MC-RR) was isolated from Dianchi Lake in China. In the molecular structure of MC-LR, glutamic acid, aspartic acid, leucine, alanine and arginine are the constitutional components which are abundant in natural water. In this paper, effects of six amino acids at their natural concentrations on the growth of the M. aeruginosa and the microcystin (MC) production were studied in batch culture. M. aeruginosa could assimilate alanine, leucine and arginine as sole nitrogen sources for growth and MC production. However, glutamic acid, aspartic acid and lysine could not be assimilated quickly, although they could pass the cell membrane and enter into the cell rapidly. Our experiment demonstrated that the possible reason of such phenomenon was that different amino acids had different effects on the process of metabolism through the free dissolved amino acids within the cells.
authors.affiliation: State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Ruihua
authors.initials: R
authors.lastname: Dai
authors.forename: Huijuan
authors.initials: H
authors.lastname: Liu
authors.forename: Jiuhui
authors.initials: J
authors.lastname: Qu
authors.forename: Xu
authors.initials: X
authors.lastname: Zhao
authors.forename: Yining
authors.initials: Y
authors.lastname: Hou
mesh_terms: D000409:Alanine / Q000737:chemistry; D000596:Amino Acids / Q000737:chemistry*; D001120:Arginine / Q000737:chemistry; D001224:Aspartic Acid / Q000737:chemistry; D001709:Biotechnology / Q000379:methods*; D000458:Cyanobacteria / Q000378:metabolism; D018698:Glutamic Acid / Q000737:chemistry; D007930:Leucine / Q000737:chemistry; D008387:Marine Toxins; D052998:Microcystins / Q000737:chemistry; D046931:Microcystis / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D009584:Nitrogen / Q000737:chemistry; D013997:Time Factors
chemical_list: D000596:Amino Acids; D008387:Marine Toxins; D052998:Microcystins; D001224:Aspartic Acid; D018698:Glutamic Acid; D001120:Arginine; C063855:microcystin RR; D007930:Leucine; D009584:Nitrogen; D000409:Alanine