title: Fibrinolytic poly(dimethyl siloxane) surfaces.
abstract: PDMS surfaces have been modified to confer both resistance to non-specific protein adsorption and clot lyzing properties. The properties and chemical compositions of the surfaces have been investigated using water contact angle measurements, ATR FT-IR spectroscopy, and XPS. The ability of the PEG component to suppress non-specific protein adsorption was assessed by measurement of radiolabeled fibrinogen uptake from buffer. The adsorption of plasminogen from human plasma to the various surfaces was studied. In vitro experiments demonstrated that lysine-immobilized surfaces with free epsilon-amino groups were able to dissolve fibrin clots, following exposure to plasma and tissue plasminogen activator. [Figure: see text].
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 122 Luoshi Rd. 430070, China. hongchen@whut.edu.cn
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Zhang
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.forename: W Glenn
authors.initials: WG
authors.lastname: McClung
authors.forename: Michael A
authors.initials: MA
authors.lastname: Brook
authors.forename: Heather
authors.initials: H
authors.lastname: Sheardown
authors.forename: John L
authors.initials: JL
authors.lastname: Brash
mesh_terms: D000327:Adsorption; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000627:therapeutic use*; D005342:Fibrinolysis; D005343:Fibrinolytic Agents / Q000737:chemistry*; D006801:Humans; D010958:Plasminogen / Q000378:metabolism; D011092:Polyethylene Glycols; D011506:Proteins; D013057:Spectrum Analysis; D013499:Surface Properties
chemical_list: D004129:Dimethylpolysiloxanes; D005343:Fibrinolytic Agents; D011506:Proteins; D011092:Polyethylene Glycols; D010958:Plasminogen