Fibrinolytic poly(dimethyl siloxane) surfaces.
PDMS surfaces have been modified to confer both resistance to non-specific protein adsorption and clot lyzing properties. The properties and chemical compositions of the surfaces have been investigated using water contact angle measurements, ATR FT-IR spectroscopy, and XPS. The ability of the PEG component to suppress non-specific protein adsorption was assessed by measurement of radiolabeled fibrinogen uptake from buffer. The adsorption of plasminogen from human plasma to the various surfaces was studied. In vitro experiments demonstrated that lysine-immobilized surfaces with free epsilon-amino groups were able to dissolve fibrin clots, following exposure to plasma and tissue plasminogen activator. [Figure: see text].
Chen
Hong
H
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, and School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 122 Luoshi Rd. 430070, China. hongchen@whut.edu.cn
Wang
Liang
L
Zhang
Yanxia
Y
Li
Dan
D
McClung
W Glenn
WG
Brook
Michael A
MA
Sheardown
Heather
H
Brash
John L
JL
D000327:Adsorption; D004129:Dimethylpolysiloxanes / Q000737:chemistry / Q000627:therapeutic use*; D005342:Fibrinolysis; D005343:Fibrinolytic Agents / Q000737:chemistry*; D006801:Humans; D010958:Plasminogen / Q000378:metabolism; D011092:Polyethylene Glycols; D011506:Proteins; D013057:Spectrum Analysis; D013499:Surface Properties
D004129:Dimethylpolysiloxanes; D005343:Fibrinolytic Agents; D011506:Proteins; D011092:Polyethylene Glycols; D010958:Plasminogen