title: Mast cell cathelicidin antimicrobial peptide prevents invasive group A Streptococcus infection of the skin.
abstract: Mast cells (MC) express cathelicidin antimicrobial peptides that act as broad-spectrum antibiotics and influence the immune defense of multiple epithelial surfaces. We hypothesized that MC help protect against skin infection through the expression of cathelicidin. The susceptibility of MC-deficient mice (Kit Wsh(-/-)) to invasive group A streptococcus (GAS) was compared with control mice. Following s.c. injection of GAS, MC-deficient mice had 30% larger skin lesions, 80% more lesional bacteria, and 30% more spleens positive for bacteria. In contrast to results obtained when GAS was injected into skin, no significant differences were noted between MC-deficient mice and control mice after GAS was applied topically, indicating that MC activity is most important after barrier penetration. To determine whether these differences were due to MC expression of cathelicidin, MC-deficient mice were reconstituted with MC derived from either wild-type or cathelicidin-deficient (Camp(-/-)) mice and challenged with GAS. Forty-eight hours after bacterial injection, mice that did not receive MC had an average lesion size of 200 mm(2), mice reconstituted with wild-type MC showed lesions comparable to control mice (25 mm(2)), while mice reconstituted with Camp(-/-) MC showed an average lesion size of 120 mm(2). Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) analysis of cathelicidin peptide purified from mast cells defined this as a unique 28-aa peptide. Combined, these results show that MC confer defense against Gram-positive bacterial infection in the skin, a function mediated in part by the expression of a unique cathelicidin peptide.
authors.affiliation: Department of Medicine, Division of Dermatology, University of California, San Diego and Veteran's Affairs Medical Center San Diego, CA 92161, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Di Nardo
authors.forename: Kenshi
authors.initials: K
authors.lastname: Yamasaki
authors.forename: Robert A
authors.initials: RA
authors.lastname: Dorschner
authors.forename: Yuping
authors.initials: Y
authors.lastname: Lai
authors.forename: Richard L
authors.initials: RL
authors.lastname: Gallo
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000172:deficiency / Q000276:immunology / Q000378:metabolism*; D016470:Bacteremia / Q000276:immunology / Q000382:microbiology; D054804:Cathelicidins; D015169:Colony Count, Microbial; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D008817:Mice, Mutant Strains; D020556:Neutrophil Infiltration; D009504:Neutrophils / Q000276:immunology; D012867:Skin / Q000276:immunology / Q000382:microbiology; D017192:Skin Diseases, Bacterial / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D013290:Streptococcal Infections / Q000276:immunology* / Q000382:microbiology / Q000517:prevention & control; D013297:Streptococcus pyogenes / Q000254:growth & development / Q000276:immunology* / Q000302:isolation & purification
chemical_list: D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins