title: Differences in DBA/1J and DBA/2J reveal lipid QTL genes.
abstract: Recent advances in mouse genomics have revealed considerable variation in the form of single-nucleotide polymorphisms (SNPs) among common inbred strains. This has made it possible to characterize closely related strains and to identify genes that differ; such genes may be causal for quantitative phenotypes. The mouse strains DBA/1J and DBA/2J differ by just 5.6% at the SNP level. These strains exhibit differences in a number of metabolic and lipid phenotypes, such as plasma levels of triglycerides (TGs) and HDL. A cross between these strains revealed multiple quantitative trait loci (QTLs) in 294 progeny. We identified significant TG QTLs on chromosomes (Chrs) 1, 2, 3, 4, 8, 9, 10, 11, 12, 13, 14, 16, and 19, and significant HDL QTLs on Chrs 3, 9, and 16. Some QTLs mapped to chromosomes with limited variability between the two strains, thus facilitating the identification of candidate genes. We suggest that Tshr is the QTL gene for Chr 12 TG and HDL levels and that Ihh may account for the TG QTL on Chr 1. This cross highlights the advantage of crossing closely related strains for subsequent identification of QTL genes.
authors.affiliation: The Jackson Laboratory, Bar Harbor, ME, USA.
authors.forename: Ioannis M
authors.initials: IM
authors.lastname: Stylianou
authors.forename: Sarah R
authors.initials: SR
authors.lastname: Langley
authors.forename: Kenneth
authors.initials: K
authors.lastname: Walsh
authors.forename: Yuan
authors.initials: Y
authors.lastname: Chen
authors.forename: Céline
authors.initials: C
authors.lastname: Revenu
authors.forename: Beverly
authors.initials: B
authors.lastname: Paigen
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D033481:Chromosomes, Mammalian; D003433:Crosses, Genetic; D005260:Female; D008075:Lipoproteins, HDL / Q000097:blood / Q000235:genetics* / Q000378:metabolism*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D019463:Mice, Inbred MRL lpr; D008814:Mice, Inbred NZB; D040641:Quantitative Trait Loci; D013045:Species Specificity; D014280:Triglycerides / Q000097:blood / Q000235:genetics* / Q000378:metabolism*
chemical_list: D008075:Lipoproteins, HDL; D014280:Triglycerides