Western blot analysis using metal-nitrilotriacetate conjugated CdSe/ZnS quantum dots.
To co-opt the remarkable optical properties and benefits of quantum dots and broadly used metal-NTA:histidine tag interactions, we generated metal-NTA conjugated quantum dots and applied them to Western blot analysis. In our hands, this application dramatically reduced the time and effort required for Western blot analysis, whereas the sensitivity was comparable to that of the conventionally available anti-histidine tag antibody. Our quantum dots were stable up to 6 months without precipitation. Interestingly, under our conditions, cobalt-NTA showed better detection ability than did nickel-NTA. Our new method may be able to facilitate and simplify the routinely used protein detection procedure.
Kim
Myung Jin
MJ
Invertebrate Modeling Laboratory, Institut Pasteur Korea, 136-791 Seoul, Korea.
Park
Hye-Young
HY
Kim
Junwon
J
Ryu
Jiyoung
J
Hong
Sukmin
S
Han
Sung-Jun
SJ
Song
Rita
R
D015153:Blotting, Western / Q000379:methods*; D019187:Cadmium Compounds / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D009571:Nitrilotriacetic Acid / Q000737:chemistry*; D011506:Proteins / Q000032:analysis / Q000737:chemistry; D045663:Quantum Dots; D015203:Reproducibility of Results; D018036:Selenium Compounds / Q000737:chemistry*; D013440:Sulfides / Q000737:chemistry*; D017967:Zinc Compounds / Q000737:chemistry*
D019187:Cadmium Compounds; D011506:Proteins; D018036:Selenium Compounds; D013440:Sulfides; D017967:Zinc Compounds; D009532:Nickel; C058667:cadmium selenide; D009571:Nitrilotriacetic Acid; C031238:zinc sulfide