title: Chromium tolerance and reduction potential of a Bacillus sp.ev3 isolated from metal contaminated wastewater.
abstract: This study was aimed at assessing the ability of Bacillus sp.ev3 to reduce hexavalent chromium into its trivalent form. Bacillus sp.ev3 could tolerate Cr(6+) (4800 microg/mL), Pb(2+) (800 microg/mL), Cu(2+) (200 microg/mL), Cd(2+) (50 microg/mL), Zn(2+) (400 microg/mL), Ni(2+) (4000 microg/mL) and Hg(2+) (50 microg/mL). Bacillus sp.ev3 showed optimum growth at 37 degrees C and pH at 7. Bacillus sp.ev3 could reduce 91% of chromium from the medium after 96 h and was also capable to reduce 84% chromium from the industrial effluents after 144 h. Cell free extracts of Bacillus sp.ev3 grown in the presence of Cr showed reduction of 70%, 45.6% and 27.4% at concentrations of 10 microg Cr(6+)/mL, 50 microg Cr(6+)/mL and 100 microg Cr(6+)/mL, respectively.
authors.affiliation: Department of Microbiology and Molecular Genetics, University of the Punjab, New Campus, Lahore 54590, Pakistan. rehman_genetics@yahoo.com
authors.forename: A
authors.initials: A
authors.lastname: Rehman
authors.forename: A
authors.initials: A
authors.lastname: Zahoor
authors.forename: B
authors.initials: B
authors.lastname: Muneer
authors.forename: S
authors.initials: S
authors.lastname: Hasnain
mesh_terms: D001407:Bacillus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002627:Chemistry, Physical; D002857:Chromium / Q000378:metabolism* / Q000633:toxicity*; D004351:Drug Resistance; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste / Q000032:analysis; D008670:Metals / Q000494:pharmacology*; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012722:Sewage / Q000032:analysis*; D013696:Temperature; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism*
chemical_list: D007220:Industrial Waste; D008670:Metals; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014874:Water Pollutants, Chemical; D002857:Chromium