title: Assignment and expression patterns of porcine muscle-specific isoform of phosphoglycerate mutase gene.
abstract: It has been reported that the muscle-specific isoform (type M, PGAM2) of phosphoglycerate mutase (PGAM) is a housekeeping enzyme; it catalyzes the conversion of 3-phosphoglycerate into 2-phosphoglycerate in the glycolysis process to release energy. It is encoded by the Pgam2 gene. In this study, the cDNA of the porcine Pgam2 was cloned. This gene contains an open reading frame of 765 bp encoding a protein of 253 residues, and the predicted protein sequences share high similarity with other mammalians, 96% identity with humans, and 94% identity with mouse and rats. Pgam2 was mapped to SSC18q13-q21 by the RH panel. In this region, there are several QTLs, such as fat ratio, lean percentage, and diameter of muscle fiber, which affect meat production and quality. The reverse transcriptase-polymerase chain reaction revealed that the porcine Pgam2 gene was mainly expressed in the muscle tissue (skeletal muscle and cardiac muscle), and was expressed highly at skeletal muscle development stages (embryonic periods: 33, 65, and 90 days post-conception (dpc); postnatal pigs: 4 days and adult). This indicates that the Pgam2 gene plays an important role in muscle growth and development. In addition, it was demonstrated that PGAM2 locates both in cytoplasm and nuclei, and takes part in the glycometabolism process of cytoplasm and nuclei.
authors.affiliation: Lab of Molecular Biology and Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Haifang
authors.initials: H
authors.lastname: Qiu
authors.forename: Shuhong
authors.initials: S
authors.lastname: Zhao
authors.forename: Xuewen
authors.initials: X
authors.lastname: Xu
authors.forename: Martine
authors.initials: M
authors.lastname: Yerle
authors.forename: Bang
authors.initials: B
authors.lastname: Liu
mesh_terms: D000818:Animals; D002460:Cell Line; D002875:Chromosomes / Q000201:enzymology; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D042541:Intracellular Space / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D009132:Muscles / Q000378:metabolism*; D009928:Organ Specificity; D010736:Phosphoglycerate Mutase / Q000235:genetics* / Q000378:metabolism; D013552:Swine; D013997:Time Factors
chemical_list: D007527:Isoenzymes; D010736:Phosphoglycerate Mutase