[Determination of the proteins in serum albumins by synchronous fluorescence technique with 3-(2-cyanoethyl) cytosine as a probe].
Under simulative biological conditions and upon the interactions between 3-(2-cyanoethyl) cytosine (CECT) and human serum albumin (HSA) or bovine serum albumin (BSA), the authors studied the characteristics of synchronous fluorescence spectra of CECT-protein system with CECT as a molecular spectral probe. The spectral characteristics and intensity of synchronous fluorescence were related to the value of deltalambda, reaction medium, reaction temperature and so on. On the basis of these, the new method for the determination of proteins in serum albumin was developed with CECT as a molecular spectral probe. Under the optimum experimental conditions, the synchronous fluorescence intensities of CECT-HSA and CECT-BSA systems were in good proportion to the concentrations of HSA and BSA in the ranges of 0-441.4 and 0-351.0 microg x mL(-1), respectively. The detection limits were 0.023 and 0.035 microg x mL(-1), respectively. Relative standard deviations located between 12% and 3.3%, and the addition standard recovery was 97.2%-100.4% for all elements. The results suggested that the method features easy of implementation, rapidity, high sensitivity, broad linear range, and better RSD and recovery etc., and was employed directly to determine the total proteins in serum albumin samples with satisfactory results.
Cui
Feng-Ling
FL
College of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China. fenglingcui@hotmail.com
Wang
Jun-Li
JL
Cui
Yan-Rui
YR
Qu
Gui-Rong
GR
Lu
Yan
Y
Fan
Jing
J
D000818:Animals; D002417:Cattle; D003596:Cytosine / Q000031:analogs & derivatives* / Q000032:analysis; D006801:Humans; D011506:Proteins / Q000032:analysis*; D012709:Serum Albumin / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*
C550013:3-(2-cyanoethyl)cytosine; D011506:Proteins; D012709:Serum Albumin; D012710:Serum Albumin, Bovine; D003596:Cytosine