Investigation of the interaction between colloidal TiO(2) and bovine hemoglobin using spectral methods.
The interaction between bovine hemoglobin (BHb) and TiO(2) colloid was investigated by UV/vis absorption, UV/vis diffuse reflectance spectrum, IR, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques under physiological pH 7.40. TiO(2) effectively quenched the intrinsic fluorescence of BHb via static quenching. The process of binding TiO(2) on BHb was a spontaneous molecular interaction procedure. The thermodynamic parameters, DeltaH degrees and DeltaS degrees were estimated to be -78.07 kJ mol(-1), -110.93 J mol(-1)K(-1) according to the van' Hoff equation. This indicates that the van der Waals and hydrogen bonds interactions played a major role in stabilizing the TiO(2)-BHb complex. The effect of TiO(2) on the conformation of BHb was analyzed using synchronous fluorescence spectroscopy.
Wang
Yan-Qing
YQ
Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Institute of Applied Chemistry and Environmental Engineering, Yancheng Teachers University, Yancheng City, People's Republic of China. wyqing76@126.com
Zhang
Hong-Mei
HM
Wang
Rong-Hua
RH
D000818:Animals; D002417:Cattle; D003102:Colloids; D006454:Hemoglobins / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D014025:Titanium / Q000737:chemistry*
D003102:Colloids; D006454:Hemoglobins; C009495:titanium dioxide; D014025:Titanium