Fast detection of Salmonella Infantis with carbon nanotube field effect transistors.
In this paper we report a fast, sensitive and label-free biosensor for the selective determination of Salmonella Infantis. It is based on a field effect transistor (FET) in which a network of single-walled carbon nantotubes (SWCNTs) acts as the conductor channel. Anti-Salmonella antibodies were adsorbed onto the SWCNTs and subsequently the SWCNTs were protected with Tween 20 to prevent the non-specific binding of other bacteria or proteins. Our FET devices were exposed to increasing concentrations of S. Infantis and were able to detect at least 100 cfu/mL in 1h. To evaluate the selectivity of our FET devices, Streptococcus pyogenes and Shigella sonnei were tested as potential competing bacteria for Salmonella. At a concentration of 500 cfu/mL, neither Streptococcus nor Shigella interfered with the detection of Salmonella. Therefore, these devices could be used as useful label-free platforms to detect S. Infantis and, by using the suitable antibody, other bacteria or viruses.
Villamizar
Raquel A
RA
Department of Analytical and Organic Chemistry, University of Rovira i Virgili, Marcel.lí Domingo s/n, 43007, Tarragona, Spain.
Maroto
Alicia
A
Rius
F Xavier
FX
Inza
Isabel
I
Figueras
Maria J
MJ
D015374:Biosensing Techniques / Q000295:instrumentation*; D015169:Colony Count, Microbial / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000648:ultrastructure*; D015203:Reproducibility of Results; D012475:Salmonella / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013499:Surface Properties; D014173:Transistors, Electronic
D037742:Nanotubes, Carbon