Regional differences in mRNA responses in blue mussels within the Baltic proper.
Mussels (Mytilus sp.) from two regions along the permanent salinity gradient within the Baltic proper were exposed to copper (35 ppb) or petrol (0.3 mL/L) for 10 days and analyzed for mRNA expressions in gill tissue. Expression of mRNAs for the heat shock proteins HSP70 and HSP90 was significantly induced by copper, but not by petrol. For the metallothioneins MT10 and MT20, regional differences in mRNA expressions could be seen. In mussels from the northern Baltic proper, MT20 expression increased 2.8 and 3.4 times, after exposure to copper and petrol, respectively. In contrast, no change could be seen in MT20 expression for mussels from the southern Baltic proper. MT10 showed a peculiar expression not previously described. For some mussels, no expression at all was detected, some showed a weak expression and for some individuals a strong expression could be seen. For the mussels from the southern Baltic proper, the number of individuals with a strong expression of MT10 increased from 1 out of 18 (control), to 7 and 8, after exposure to copper and petrol, respectively. The results clearly show that responses vary between different regions within the Baltic proper, which emphasises the importance to study interactions between contaminants, populations and regions.
Lilja
Karl
K
School of Life Sciences, Södertörn University College SE-141 89 Huddinge, Sweden. karl.lilja@sh.se
Prevodnik
Andreas
A
Gardeström
Johanna
J
Elfwing
Tina
T
Tedengren
Michael
M
Bollner
Tomas
T
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003001:Cloning, Molecular; D003300:Copper / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000235:genetics; D008668:Metallothionein / Q000235:genetics; D049880:Mytilus edulis / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D010578:Petroleum / Q000633:toxicity*; D012333:RNA, Messenger / Q000378:metabolism*; D012623:Seawater; D013548:Sweden; D014158:Transcription, Genetic / Q000187:drug effects*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D010578:Petroleum; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D003300:Copper; D008668:Metallothionein