Systematic evaluation of FRAP experiments performed in a confocal laser scanning microscope--part II: Multiple diffusion processes.
A procedure for a systematic evaluation of fluorescence recovery after photo-bleaching (FRAP) data is presented that allows one to determine distributions of diffusion coefficients. The method provides a straightforward and calibration-free way to quantify multiple diffusion processes when FRAP is measured as a function of time and space by means of confocal laser scanning microscopy. It is verified utilizing FRAP experiments on mixtures of differently sized fluorescent micro-spheres as realizations of discrete D distributions.
Hauser
G I
GI
Institute of Physical Chemistry, Clausthal University of Technology, Arnold-Sommerfeld-Strasse 4, D-38678 Clausthal-Zellerfeld, Germany.
Seiffert
S
S
Oppermann
W
W
D004058:Diffusion; D005069:Evaluation Studies as Topic; D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D008863:Microspheres; D011137:Polystyrenes / Q000737:chemistry
D011137:Polystyrenes