title: Effects of amide constituents from pepper on adipogenesis in 3T3-L1 cells.
abstract: Several amide constituents (piperlonguminine and retrofractamides A, B, and C) from the fruit of Piper chaba promoted adipogenesis of 3T3-L1 cells. Among them, retrofractamide A was the most active and significantly increased the amount of adiponectin released into the medium and the uptake of 2-deoxyglucose into the cells. Retrofractamide A also increased mRNA levels of adiponectin, peroxisome proliferator-activated receptor gamma2 (PPARgamma2), glucose transporter 4 (GLUT4), and insulin receptor substrate 1 (IRS-1), but did not act as a PPARgamma agonist different from troglitazone.
authors.affiliation: Kyoto Pharmaceutical University, Misasagi, Yamashina-ku, Kyoto 607-8412, Japan.
authors.forename: Hailong
authors.initials: H
authors.lastname: Zhang
authors.forename: Hisashi
authors.initials: H
authors.lastname: Matsuda
authors.forename: Seikou
authors.initials: S
authors.lastname: Nakamura
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yoshikawa
mesh_terms: D041721:3T3-L1 Cells; D048868:Adaptor Proteins, Signal Transducing / Q000187:drug effects; D050156:Adipogenesis / Q000187:drug effects*; D052242:Adiponectin / Q000378:metabolism; D000577:Amides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D052117:Benzodioxoles / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D004148:Dioxolanes / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D051275:Glucose Transporter Type 4 / Q000187:drug effects; D055504:Insulin Receptor Substrate Proteins; D051379:Mice; D047492:Peroxisome Proliferator-Activated Receptors / Q000187:drug effects; D029222:Piper nigrum / Q000737:chemistry*; D010881:Piperidones
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D052242:Adiponectin; D000577:Amides; D052117:Benzodioxoles; D004148:Dioxolanes; D051275:Glucose Transporter Type 4; D055504:Insulin Receptor Substrate Proteins; D047492:Peroxisome Proliferator-Activated Receptors; C526295:Irs1 protein, mouse; C454279:retrofractamide A; C498077:piperlongumine; D010881:Piperidones