title: [Construction of the recombinant adenovirus vector bearing dual-survivin shRNA].
abstract: OBJECTIVE
  To construct a recombinant adenovirus vector bearing dual-survivin short hairpin RNA (shRNA).
  
  
  METHODS
  Dual-survivin shRNA was designed and synthesized respectively, both inserted into adenovirus DNA. The recombinant adenovirus vector was confirmed via both sequencing and restriction digestion analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenoviruses.
  
  
  RESULTS
  The recombinant adenovirus vector was constructed and the target sequence was obtained.
  
  
  CONCLUSIONS
  The construction of the recombinant adenovirus vector provides a basis for the research of potential gene therapy for prostate cancer.
authors.affiliation: Department of Urology, Second Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215004, China. ag2525@tom.com
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Yu-Xi
authors.initials: YX
authors.lastname: Shan
authors.forename: Dong-Rong
authors.initials: DR
authors.lastname: Yang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D000077022:Survivin; D014162:Transfection
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D000077022:Survivin; D049452:Green Fluorescent Proteins; D004262:DNA Restriction Enzymes