title: CD13 is a novel mediator of monocytic/endothelial cell adhesion.
abstract: During inflammation, cell surface adhesion molecules guide the adhesion and migration of circulating leukocytes across the endothelial cells lining the blood vessels to access the site of injury. The transmembrane molecule CD13 is expressed on monocytes and endothelial cells and has been shown to mediate homotypic cell adhesion, which may imply a role for CD13 in inflammatory monocyte trafficking. Here, we show that ligation and clustering of CD13 by mAb or viral ligands potently induce myeloid cell/endothelial adhesion in a signal transduction-dependent manner involving monocytic cytoskeletal rearrangement and filopodia formation. Treatment with soluble recombinant (r)CD13 blocks this CD13-dependent adhesion, and CD13 molecules from monocytic and endothelial cells are present in the same immunocomplex, suggesting a direct participation of CD13 in the adhesive interaction. This concept is strengthened by the fact that activated monocytic cells adhere to immobilized recombinant CD13. Furthermore, treatment with anti-CD13 antibodies in a murine model of peritonitis results in a decrease in leukocyte infiltration into the peritoneum, suggesting a potential role for CD13 in leukocyte trafficking in vivo. Therefore, this work supports a new direction for CD13 biology, where these cell surface molecules act as true molecular interfaces that induce and participate in critical inflammatory cell interactions.
authors.affiliation: Center for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, 263 Farmington Ave., Farmington, CT 06030-3501, USA.
authors.forename: Paola
authors.initials: P
authors.lastname: Mina-Osorio
authors.forename: Beata
authors.initials: B
authors.lastname: Winnicka
authors.forename: Catherine
authors.initials: C
authors.lastname: O'Conor
authors.forename: Christina L
authors.initials: CL
authors.lastname: Grant
authors.forename: Lotte K
authors.initials: LK
authors.lastname: Vogel
authors.forename: Daniel
authors.initials: D
authors.lastname: Rodriguez-Pinto
authors.forename: Kathryn V
authors.initials: KV
authors.lastname: Holmes
authors.forename: Enrique
authors.initials: E
authors.lastname: Ortega
authors.forename: Linda H
authors.initials: LH
authors.lastname: Shapiro
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000494:pharmacology; D015703:Antigens, CD / Q000502:physiology; D018826:CD13 Antigens / Q000276:immunology / Q000494:pharmacology / Q000502:physiology*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D004195:Disease Models, Animal; D004730:Endothelium, Vascular / Q000187:drug effects / Q000502:physiology*; D005260:Female; D006801:Humans; D051379:Mice; D008815:Mice, Inbred Strains; D009000:Monocytes / Q000187:drug effects / Q000502:physiology*; D010538:Peritonitis / Q000503:physiopathology; D011994:Recombinant Proteins / Q000494:pharmacology; D014471:Umbilical Veins / Q000502:physiology
chemical_list: D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D011994:Recombinant Proteins; D018826:CD13 Antigens