title: Determination of vitamin E and carotenoid pigments by high performance liquid chromatography in shell of Chionoecetes opilio.
abstract: This study reports the optimization of a method for the determination of vitamin E and carotenoids in shells of Chionoecetes opilio samples by online HPLC coupled with UV-vis and fluorescence detectors. The carotenoids were determined with diode-array detector (lambda 450 nm) and vitamin E with fluorescence detection (lambda(ex) 288, lambda(em) 331 nm). Two extractions methods were compared, saponification followed by an extraction step and a simple extraction with acetone. The last one was selected because allows to determine all compounds. Linearity, precisions and recoveries achieved for all compounds were satisfactory. Mean concentrations (mg per 100 g dry weight) were; 23.3 for vitamin E, 9.49 for astaxanthin and 0.2 mg for beta-carotene.
authors.affiliation: Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Vilasoa-Martínez
authors.forename: Carina
authors.initials: C
authors.lastname: Calaza-Ramos
authors.forename: Julia
authors.initials: J
authors.lastname: López-Hernández
authors.forename: María Asunción
authors.initials: MA
authors.lastname: Lage-Yusty
authors.forename: Perfecto Paseiro
authors.initials: PP
authors.lastname: Losada
authors.forename: Ana
authors.initials: A
authors.lastname: Rodríguez-Bernaldo de Quirós
mesh_terms: D000818:Animals; D003386:Brachyura / Q000737:chemistry* / Q000145:classification*; D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010860:Pigments, Biological / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*
chemical_list: D010860:Pigments, Biological; D014810:Vitamin E; D002338:Carotenoids