title: The Burkholderia mallei BmaR3-BmaI3 quorum-sensing system produces and responds to N-3-hydroxy-octanoyl homoserine lactone.
abstract: Burkholderia mallei has two acyl-homoserine lactone (acyl-HSL) signal generator-receptor pairs and two additional signal receptors, all of which contribute to virulence. We show that B. mallei produces N-3-hydroxy-octanoyl HSL (3OHC8-HSL) but a bmaI3 mutant does not. Recombinant Escherichia coli expressing BmaI3 produces hydroxylated acyl-HSLs, with 3OHC8-HSL being the most abundant compound. In recombinant E. coli, BmaR3 responds to 3OHC8-HSL but not to other acyl-HSLs. These data indicate that the signal for BmaR3-BmaI3 quorum sensing is 3OHC8-HSL.
authors.affiliation: Department of Microbiology, University of Washington School of Medicine, Seattle, Washington 98195-7242, USA.
authors.forename: Breck A
authors.initials: BA
authors.lastname: Duerkop
authors.forename: Jake P
authors.initials: JP
authors.lastname: Herman
authors.forename: Ricky L
authors.initials: RL
authors.lastname: Ulrich
authors.forename: Mair E A
authors.initials: ME
authors.lastname: Churchill
authors.forename: E Peter
authors.initials: EP
authors.lastname: Greenberg
mesh_terms: D015107:4-Butyrolactone / Q000031:analogs & derivatives* / Q000096:biosynthesis; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D042726:Burkholderia mallei / Q000502:physiology*; D004926:Escherichia coli / Q000235:genetics; D009154:Mutation; D053038:Quorum Sensing; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; C511789:N-(3-oxooctanoyl)homoserine lactone; D011994:Recombinant Proteins; D015107:4-Butyrolactone