title: Ultra-sensitive detection of bacterial toxin with silicon nanowire transistor.
abstract: Nanowire field effect transistors (nano-FET) were lithographically fabricated using 50 nm doped polysilicon nanowires attached to two small gold terminals separated from each other by a approximately 150 nm gap to serve as the basis for electronic detection of bacteria toxins. The device characterizations, semiconducting properties and use in a robust and sensitive bio-molecular detection sensor of bacterial toxins were reported in this work. The device characteristics were demonstrated with varying gate and drain voltages. The bio-molecular detection was demonstrated using electrochemical impedance spectroscopy (EIS), using Staphylococcus aureus Enterotoxin B (SEB) as the target molecule. The detection limit of SEB was observed in the range of 10-35 fM.
authors.affiliation: Center for Advanced Microelectronics and Biomolecular Research, University of Idaho, Post Falls, ID 83854, USA. nmishra@cambr.uidaho.edu
authors.forename: Nirankar N
authors.initials: NN
authors.lastname: Mishra
authors.forename: Wusi C
authors.initials: WC
authors.lastname: Maki
authors.forename: Eric
authors.initials: E
authors.lastname: Cameron
authors.forename: Ron
authors.initials: R
authors.lastname: Nelson
authors.forename: Paul
authors.initials: P
authors.lastname: Winterrowd
authors.forename: Shiva K
authors.initials: SK
authors.lastname: Rastogi
authors.forename: Brian
authors.initials: B
authors.lastname: Filanoski
authors.forename: Gary K
authors.initials: GK
authors.lastname: Maki
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis* / Q000633:toxicity; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry; D004768:Enterotoxins / Q000032:analysis / Q000633:toxicity; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D014173:Transistors, Electronic
chemical_list: D001427:Bacterial Toxins; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal; D006046:Gold; D012825:Silicon