Free-radical-scavenging principles from Phlomis caucasica.
Seven free-radical-scavenging phenolic compounds including five flavonoids, rutin (1), chrysoeriol 7-O-rutinoside (2), kaempferol 3-O-glucoside (3), chrysoeriol 7-O-glucoside (4) and naringenin (5), and two phenylethanoid glycosides, forsythoside B (6) and acteoside (7) were isolated from the methanol extract of the aerial parts of the Iranian medicinal plant Phlomis caucasica by reversed-phase preparative high-performance liquid chromatography (HPLC), and the structures of these compounds were elucidated by spectroscopic means. The free-radical-scavenging properties of 1-7 were assessed by 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay. Among these compounds, forsythoside B (6) and acteoside (7) were found to be the most potent antioxidants with the RC(50) values of 4.97 and 4.27 microg/ml, respectively.
Delazar
Abbas
A
School of Pharmacy and Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Sabzevari
Araz
A
Mojarrab
Mahdi
M
Nazemiyeh
Hossein
H
Esnaashari
Solmaz
S
Nahar
Lutfun
L
Razavi
Seied Mehdi
SM
Sarker
Satyajit Dey
SD
D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D001713:Biphenyl Compounds; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000302:isolation & purification / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D006027:Glycosides / Q000302:isolation & purification / Q000494:pharmacology; D007492:Iran; D008519:Medicine, Traditional; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology*; D031345:Phlomis / Q000737:chemistry*; D010851:Picrates; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology*; D013057:Spectrum Analysis
D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D016166:Free Radical Scavengers; D006027:Glycosides; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; C004931:1,1-diphenyl-2-picrylhydrazyl