Enzymatic microreactors for the determination of ethanol by an automatic sequential injection analysis system.
A sequential injection analysis system with two enzymatic microreactors for the determination of ethanol has been designed. Alcohol oxidase and horseradish peroxidase were separately immobilized on glass aminopropyl beads, and packed in 0.91-mL volume microreactors, working in line with the sequential injection analysis system. A stop flow of 120 s was selected for a linear ethanol range of 0.005-0.04 g/L +/- 0.6% relative standard deviation with a throughput of seven analyses per hour. The system was applied to measure ethanol concentrations in samples of distilled and nondistilled alcoholic beverages, and of alcoholic fermentation with good performance and no significant difference compared with other analytical procedures (gas chromatography and high-performance liquid chromatography).
Alhadeff
Eliana M
EM
Escola de Química, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Cidade Universitária, CEP: 21.949-900, Rio de Janeiro, Brasil. ema@eq.ufrj.br
Salgado
Andrea M
AM
Cos
Oriol
O
Pereira
Nei
N
Valdman
Belkis
B
Valero
Francisco
F
D000429:Alcohol Oxidoreductases / Q000737:chemistry*; D019149:Bioreactors / Q000382:microbiology*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004800:Enzymes, Immobilized / Q000737:chemistry; D004867:Equipment Design; D019544:Equipment Failure Analysis; D000431:Ethanol / Q000032:analysis* / Q000378:metabolism*; D017022:Flow Injection Analysis / Q000295:instrumentation*; D006735:Horseradish Peroxidase / Q000737:chemistry*; D012371:Robotics / Q000295:instrumentation; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D013053:Spectrophotometry / Q000295:instrumentation
D004800:Enzymes, Immobilized; D000431:Ethanol; D000429:Alcohol Oxidoreductases; C019643:alcohol oxidase; D006735:Horseradish Peroxidase