title: Effects of endothelial basement membrane on neutrophil adhesion and migration.
abstract: The influence of the sub-endothelial basement membrane (BM) on the adhesion and migration of leukocytes is not well-defined. We therefore investigated the behaviour of human neutrophils on purified BM proteins and on BM deposited by short- or long-term cultures of endothelial cells (EC). The adhesion, but not migration velocities, of neutrophils activated with interleukin-8 was dependent on the coating concentrations of purified collagen, laminin or fibronectin. In contrast, adhesion was similar on matrices deposited by 3-day or 20-day cultures of EC, but neutrophils migrated more slowly on the distinct BM that formed over 20 days. In addition, while adhesion on all surfaces was greatly reduced when neutrophils were treated with antibody against beta(2)-integrins, antibody against beta(1)-integrins only inhibited adhesion to the 20-day BM. Thus, the native BM has distinct effects on integrin usage and migration by neutrophils, which are not reproduced by purified proteins or matrix deposited early during endothelial culture.
authors.affiliation: Centre for Cardiovascular Sciences and MRC Centre for Immune Regulation, The Medical School, The University of Birmingham, Birmingham B15 2TT, UK.
authors.forename: Lynn M
authors.initials: LM
authors.lastname: Butler
authors.forename: Saif
authors.initials: S
authors.lastname: Khan
authors.forename: G
authors.initials: G
authors.lastname: Ed Rainger
authors.forename: Gerard B
authors.initials: GB
authors.lastname: Nash
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D001485:Basement Membrane / Q000378:metabolism*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D018929:Cell Culture Techniques; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D004727:Endothelium / Q000502:physiology*; D005109:Extracellular Matrix / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D039641:Integrin beta Chains / Q000187:drug effects / Q000378:metabolism; D016209:Interleukin-8 / Q000494:pharmacology; D007797:Laminin / Q000378:metabolism; D009504:Neutrophils / Q000187:drug effects / Q000502:physiology*; D013997:Time Factors; D014471:Umbilical Veins / Q000166:cytology / Q000378:metabolism
chemical_list: D000906:Antibodies; D005353:Fibronectins; D039641:Integrin beta Chains; D016209:Interleukin-8; D007797:Laminin; D003094:Collagen