[Anticoagulant activity of low-molecular-weight sulfated derivatives of galactomannan from Cyamopsis tetragonoloba (L.) seeds].
Galactomannan from seeds of Cyamopsis tetragonoloba (L.) Taub. (guar) was depolymerized using immobilized enzymatic preparation celloviridin. A set of fragments whose molecular weights varied from 12.6 to 245.6 kDa was obtained. Sulfated derivatives of components of all fractions were synthesized, in which the content of HSO3(-) groups was 48.05% +/- 2.31. All preparations exhibited anticoagulant activity, which was recorded in vitro in two tests--aIIa and aXa. The antithrombin activity (aIIa) was high (up to 65-87 U/mg) and did not depend on the molecular weight of a sulfated derivative; in the second test (aXa), the effect of molecular weight was observed. Biospecific electrophoresis allowed us to detect the ability of galactomannan sulfates to form complexes with protamine sulfate, a classic antidote to heparin.
Mestechkina
N M
NM
Shcherbukhin
V D
VD
Bannikova
G E
GE
Varlamov
V P
VP
Drozd
N N
NN
Tolstenkov
A S
AS
Makarov
V A
VA
Tikhonov
V E
VE
D000925:Anticoagulants / Q000138:chemical synthesis / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects*; D031293:Cyamopsis / Q000737:chemistry*; D005690:Galactose / Q000031:analogs & derivatives; D006026:Glycoside Hydrolases / Q000378:metabolism; D006868:Hydrolysis; D008351:Mannans / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D008970:Molecular Weight; D011479:Protamines / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D013431:Sulfates / Q000138:chemical synthesis / Q000378:metabolism / Q000494:pharmacology*
D000925:Anticoagulants; D008351:Mannans; D011479:Protamines; D013431:Sulfates; C012990:galactomannan; D006026:Glycoside Hydrolases; D005690:Galactose