title: Determination of trace levels of benzodiazepine in urine using capillary electrochromatography-time of flight mass spectrometry.
abstract: This paper details a method for the separation and determination of ten benzodiazepines in urine using CEC-MS(TOF) and an hexyl acrylate-based porous monolith. The TOF mass spectrometer provides an exact mass of protonated benzodiazepines to three decimal places (1-6 ppm). This high selectivity along with the CEC separation, provides an effective method for the identification of benzodiazepines. Linearity is satisfactory for all compounds in the concentration range of 25-500 ng/mL for lorazepam and 12.5-500 ng/mL for the others. The RSDs are between 1.4-2.3% for retention times and 1.1-9.2% for relative areas. Using the monolithic stationary phase, a preconcentration step is achievable and permits an 75-140-fold improvement in sensitivity. This strategy permits the quantification of these drugs down to 1 ng/mL in urine. This method was used for the analysis of benzodiazepines in spiked urine samples.
authors.affiliation: Chemistry Department, Florida International University, Miami, FL 33199, USA.
authors.forename: Maximilien
authors.initials: M
authors.lastname: Blas
authors.forename: Bruce R
authors.initials: BR
authors.lastname: McCord
mesh_terms: D000179:Acrylates; D014151:Anti-Anxiety Agents / Q000737:chemistry / Q000652:urine; D001569:Benzodiazepines / Q000737:chemistry / Q000652:urine*; D053801:Capillary Electrochromatography / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D006801:Humans; D008855:Microscopy, Electron, Scanning; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000706:statistics & numerical data
chemical_list: D000179:Acrylates; D014151:Anti-Anxiety Agents; D001569:Benzodiazepines; C526535:hexyl acrylate