title: Sympathetic regulation of the renal functions in rats reciprocally congenic for chromosome 1 blood pressure quantitative trait locus.
abstract: The role of the chromosome 1 blood pressure quantitative trait locus (QTL) on the sympathorenal interaction was studied using congenic strains. The two reciprocal congenic strains, WKYpch1.0 and SHRSPwch1.0, were respectively constructed by introgressing the stroke-prone spontaneously hypertensive rat (SHRSP)-derived fragment for the QTL into a Wistar-Kyoto rat (WKY) and vice versa. The role of the sympathetic nervous system in the kidney was evaluated by comparing the renal functions between denervated and sham-operated kidneys under anesthesia. The denervation was performed by stripping the adventitia off and applying 10% phenol to the blood vessels at the left renal hilus. Polyfructosan was continuously injected intravenously to determine the renal plasma flow and the glomerular filtration rate. A reciprocal and significant alteration in the renal norepinephrine (NE) content was observed between WKY and WKYpch1.0 and between SHRSP and SHRSPwch1.0. Concomitantly, the renal vascular resistance differed significantly between the congenic and the background parental strains. By contrast, no significant difference was observed in the fractional excretion of sodium, an index of the tubular function. While the denervation elicited a significant decrease of the renal NE content in all of the four strains studied, the significant effects of the denervation on the renal functions were observed only in SHRSP and WKYpch1.0, both of which harbored the SHRSP-derived QTL fragment. These results indicated that the chromosome 1 blood pressure QTL modulated the renal functions through the sympathetic nerve activity in the kidney.
authors.affiliation: Fourth Department of Internal Medicine, and Central Laboratory of the University Hospital, Shimane University School of Medicine, Izumo, Japan.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Toru
authors.initials: T
authors.lastname: Nabika
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Notsu
authors.forename: Toshikazu
authors.initials: T
authors.lastname: Takabatake
mesh_terms: D000818:Animals; D020296:Animals, Congenic; D001794:Blood Pressure / Q000235:genetics* / Q000502:physiology; D002875:Chromosomes / Q000235:genetics / Q000502:physiology; D004195:Disease Models, Animal; D006973:Hypertension / Q000235:genetics* / Q000503:physiopathology; D007668:Kidney / Q000098:blood supply / Q000294:innervation* / Q000502:physiology*; D040641:Quantitative Trait Loci / Q000235:genetics* / Q000502:physiology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D020521:Stroke / Q000235:genetics* / Q000503:physiopathology; D013564:Sympathetic Nervous System / Q000502:physiology*; D014655:Vascular Resistance / Q000502:physiology