Control of mRNA degradation in trypanosomes.
Control of gene expression in trypanosomes relies almost exclusively on post-transcriptional mechanisms. Trypanosomes have the normal enzymes for mRNA decay: both the exosome and a 5'-3'-exoribonuclease are important in the degradation of very unstable transcripts, whereas the CAF1/NOT complex plays a major role in the degradation of all mRNAs tested. Targeted RNA interference screening was used to identify RNA-binding proteins that regulate mRNA degradation, and it revealed roles for proteins with RNA recognition motifs or pumilio domains.
Clayton
Christine
C
ZMBH (Zentrum für Molekulare Biologie der Universität Heidelberg), Im Neuenheimer Feld 282, D69120 Heidelberg, Germany. cclayton@zmbh.uni-heidelberg.de
Schwede
Angela
A
Stewart
Mhairi
M
Robles
Ana
A
Benz
Corinna
C
Po
June
J
Wurst
Martin
M
Queiroz
Rafael
R
Quieroz
Rafael
R
Archer
Stuart
S
D000818:Animals; D007700:Kinetics; D012323:RNA Processing, Post-Transcriptional; D020871:RNA Stability; D016601:RNA-Binding Proteins / Q000378:metabolism; D014345:Trypanosoma / Q000201:enzymology / Q000378:metabolism*
D016601:RNA-Binding Proteins