title: Filarial glutathione-S-transferase: a potential vaccine candidate against lymphatic filariasis.
abstract: Present report enumerates the vaccine potential of a glutathione-S-transferase purified from Setaria cervi against lymphatic filariasis. In jirds (Meriones unguiculatus) vaccination trial, a very significant 82.75% (p<0.005) reduction in adult parasite burden was observed in ScGST immunized group after 90 days post Brugia malayi L3 challenge. An inverse correlation between the antibody level and worm burden was found in ScGST immunized group (Person's correlation r=0.943, p<0.05). No recoveries of worms were obtained in heart and lungs of vaccinated group. The Antibodies reactive to ScGST appeared within four weeks of first dose and were able to neutralize the GST activity up to 86%. In an earlier study we have shown vaccine potential of ScGST against B. malayi by ADCC. Evaluation of cytokine profile in T-cells isolated from BALB/c mice immunized with ScGST were also showed predominance of Th2 response which further maintained the humoral immunity generated by ScGST administration in mice. The overall observations prompted us to envisage ScGST as a potential vaccine candidate against lymphatic filariasis.
authors.affiliation: Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, Uttar Pradesh, India. surathaur@rediffmail.com
authors.forename: Sushma
authors.initials: S
authors.lastname: Rathaur
authors.forename: Marshleen
authors.initials: M
authors.lastname: Yadav
authors.forename: Sarika
authors.initials: S
authors.lastname: Gupta
authors.forename: V
authors.initials: V
authors.lastname: Anandharaman
authors.forename: Maryada Venkatarami
authors.initials: MV
authors.lastname: Reddy
mesh_terms: D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D017178:Brugia malayi / Q000276:immunology; D049109:Cell Proliferation; D016207:Cytokines / Q000276:immunology; D004605:Elephantiasis, Filarial / Q000276:immunology / Q000517:prevention & control*; D005260:Female; D005849:Gerbillinae / Q000469:parasitology; D005982:Glutathione Transferase / Q000276:immunology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016052:Protozoan Vaccines / Q000276:immunology*; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D016207:Cytokines; D016052:Protozoan Vaccines; D005982:Glutathione Transferase