[Amplification methods of DNA from paraffin-embedded tissues of oral leukoplakia].
OBJECTIVE
To set up a feasible method for detection of objective genes from paraffin-embedded tissues of oral leukoplakia (OLK).


METHODS
Twenty-five pieces of formalin-fixed, paraffin-embedded tissues of OSCC were selected and ATM gene was detected respectively by 3 methods: the microdissection-nested PCR method, proteinase K-PCR method and conventional phenol-chloroform-PCR method. The positivity rates were compared statistically.


RESULTS
The positivity rates of these 3 methods were 84%, 52% and 64% respectively. Significant difference was found in positivity rate between the microdissection-nested PCR method and the proteinase K-PCR method (P = 0.032).


CONCLUSIONS
The microdissection-nested PCR method merits recommendation because it is more efficient, easy to perform and has the advantage of less sample amount.
He
Yuan
Y
Department of Oral Medicine, West China School of Stomatology, Sichuan University, Chengdu 610041, China.
Chen
Qian-ming
QM
Li
Bing-qi
BQ
D064007:Ataxia Telangiectasia Mutated Proteins; D018797:Cell Cycle Proteins / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D006801:Humans; D007972:Leukoplakia, Oral / Q000235:genetics*; D016612:Paraffin Embedding; D016133:Polymerase Chain Reaction / Q000379:methods*; D017346:Protein Serine-Threonine Kinases / Q000235:genetics; D025521:Tumor Suppressor Proteins / Q000235:genetics
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; C576324:ATM protein, human; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases