Involvement of stretch-activated ion channels in strain-regulated glycosaminoglycan synthesis in mesenchymal stem cell-seeded 3D scaffolds.
The objective of this study was to determine if cyclic tensile strain would regulate the rate of glycosaminoglycan synthesis via stretch-activated ion channels in adult mesenchymal stem cells seeded in a collagen type I-glycosaminoglycan scaffold and treated with TGF-beta1. The application of 10% cyclic tensile loading at 1Hz for 7 days significantly increased the rate of glycosaminoglycan synthesis, as assessed using [(35)S] sulphate incorporation. This increase was attenuated in the presence of a stretch-activated ion channel inhibitor (10microM gadolinium chloride) demonstrating the involvement, in part, of these ion channels in the mechanotransduction pathway that couples cyclic tensile loading to matrix synthesis.
McMahon
Louise A
LA
Trinity Centre for Bioengineering, School of Engineering, Trinity College, Dublin 2, Ireland.
Campbell
Veronica A
VA
Prendergast
Patrick J
PJ
D000818:Animals; D001696:Biomechanical Phenomena; D002454:Cell Differentiation; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis*; D007473:Ion Channels / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D006025:Glycosaminoglycans; D007473:Ion Channels