title: Blood outgrowth endothelial cells from chronic myeloid leukaemia patients are BCR/ABL1 negative.
abstract: The existence of adult haemangioblasts with dual haematopoietic and endothelial developmental potential was confirmed after detection of Ph(+) vascular endothelial cells in chronic myeloid leukaemia (CML) patients. Blood outgrowth endothelial cells (OECs) from CML patients were found not to harbour the Philadelphia translocation and were thus not clonally related to BRC/ABL1(+) hematopoietic progenitors, but comprised a distinct subfraction of endothelial cells. Remarkably, the frequency of CML-derived OECs was 9-fold higher as compared to healthy donors (n = 19 and n = 300, respectively; P < 0.0001) and these cells showed increased proliferative potential, possibly reflecting the mobilisation of OEC progenitors by pro-angiogenic cytokines.
authors.affiliation: Department of Internal Medicine II, Cancer Centre, University Hospital Hamburg-Eppendorf, Hamburg, Germany.
authors.forename: Jasmin
authors.initials: J
authors.lastname: Otten
authors.forename: Alexander
authors.initials: A
authors.lastname: Schultze
authors.forename: Philippe
authors.initials: P
authors.lastname: Schafhausen
authors.forename: Svenja
authors.initials: S
authors.lastname: Otterstetter
authors.forename: Judith
authors.initials: J
authors.lastname: Dierlamm
authors.forename: Carsten
authors.initials: C
authors.lastname: Bokemeyer
authors.forename: Tim H
authors.initials: TH
authors.lastname: Brummendorf
authors.forename: Walter
authors.initials: W
authors.lastname: Fiedler
authors.forename: Sonja
authors.initials: S
authors.lastname: Loges
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism; D042783:Endothelial Cells / Q000473:pathology*; D016313:Genes, abl; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000473:pathology; D016133:Polymerase Chain Reaction
chemical_list: D014408:Biomarkers, Tumor