title: The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in Escherichia coli.
abstract: We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.
authors.affiliation: Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom.
authors.forename: James W
authors.initials: JW
authors.lastname: Donald
authors.forename: Matthew G
authors.initials: MG
authors.lastname: Hicks
authors.forename: David J
authors.initials: DJ
authors.lastname: Richardson
authors.forename: Tracy
authors.initials: T
authors.lastname: Palmer
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003580:Cytochromes / Q000032:analysis* / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D021381:Protein Transport; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D020592:Shewanella / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D003580:Cytochromes; D011994:Recombinant Proteins