Surface plasmon resonance study of vesicle rupture by virus-mimetic attack.
Frank and coworkers [N. J. Cho, S. J. Cho, K. H. Cheong, J. S. Glenn and C. W. Frank, J. Am. Chem. Soc., 2007, 129, 10050] investigated what happens when lipid vesicles made of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine), which serves as a mimic for cell membranes, are exposed to the amphipathic helix peptide, PEP1, which is of the same type found in hepatitis C virus. Using atomic force field microscopy and quartz crystal microbalance measurements they presented evidence that the vesicle is transformed into a lipid bilayer. We use surface plasmon resonance (SPR) microscopy to follow this process in real time. We find an induction period (intermediate state) of approximately 10-min duration between the time of membrane binding and membrane rupture. The SPR data support the interpretation that a lipid bilayer is formed and allow us to put forward a mechanism for the vesicle-rupture event. As a side benefit, we demonstrate how to build two-dimensional lipid patterns on a gold surface using this vesicle-rupture process.
Chah
Soonwoo
S
Department of Chemistry, Stanford University, Stanford, CA 94305-5080, USA.
Zare
Richard N
RN
D032701:Biomimetics; D002627:Chemistry, Physical / Q000379:methods; D003460:Crystallization; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D016174:Hepacivirus / Q000648:ultrastructure; D008051:Lipid Bilayers / Q000737:chemistry; D008055:Lipids / Q000737:chemistry; D010455:Peptides / Q000737:chemistry; D010713:Phosphatidylcholines / Q000737:chemistry; D011791:Quartz / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D014780:Viruses / Q000737:chemistry*
D008051:Lipid Bilayers; D008055:Lipids; D010455:Peptides; D010713:Phosphatidylcholines; D011791:Quartz; D006046:Gold; C028694:1-palmitoyl-2-oleoylphosphatidylcholine