Effect of recombinant TRAIL in a murine co-culture system of osteoclastogenesis.
Although some experimental evidence has implicated the TRAIL/TRAIL-receptor system in the regulation of osteoclastogenesis, the only available studies performed so far have been performed on isolated pre-osteoclasts, induced to differentiate by the addition of recombinant RANKL and M-CSF. Using a more physiological co-culture system in the absence of exogenous cytokines, we have here demonstrated that recombinant TRAIL inhibits osteoclast formation, but only at relatively high concentrations (500 ng/mL).
Nicolin
V
V
Narducci
P
P
Celeghini
C
C
D000818:Animals; D018920:Coculture Techniques; D006801:Humans; D016173:Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D051379:Mice; D010010:Osteoclasts / Q000166:cytology / Q000502:physiology*; D053244:Osteoprotegerin / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism*
D053244:Osteoprotegerin; D053245:RANK Ligand; D011994:Recombinant Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D016173:Macrophage Colony-Stimulating Factor