title: Ex vivo antioxidant effects of D-004, a lipid extract from Roystonea regia fruits, on rat prostate tissue.
abstract: OBJECTIVE
  To investigate whether oral treatment with D-004, a lipid extract of the Cuban royal palm fruit, produces antioxidant effects in the prostate tissue of normal and testosterone (T)-treated rats.
  
  
  METHODS
  In our first experiment, normal rats were distributed into five groups: one group treated with the vehicle and four groups treated with D-004 (100, 200, 400 or 800 mg/kg). In our second experiment, rats were randomized into five groups: a negative control group and four T-injected groups. The latter were comprised of a positive control group treated with the vehicle, and three groups treated with D-004 (200, 400 or 800 mg/kg).
  
  
  RESULTS
  In normal rats, D-004 (100-800 mg/kg) inhibited significantly and dose-dependently iron-initiated malondialdehyde (MDA) accumulation in prostate homogenates (35.7%-80.0%) vs the controls. D-004 (200-800 mg/kg) significantly reduced baseline MDA and carbonyl groups in prostate homogenates of normal rats to approximately 80% and 50%, respectively, and totally (100%) in T-treated rats.
  
  
  CONCLUSIONS
  Oral treatment with D-004 reduced MDA and carbonyl groups dose-dependently and markedly in normal and T-injected rats. These findings show that D-004 given at doses effective to prevent prostate hyperplasia also produces antioxidant effects in the prostate tissue.
authors.affiliation: Laboratory of Biochemistry, Center of Natural Products, National Center for Scientific Research, Havana City, Cuba. yohani.perez@cnic.edu.cu
authors.forename: Yohani
authors.initials: Y
authors.lastname: Perez
authors.forename: Vivian
authors.initials: V
authors.lastname: Molina
authors.forename: Rosa
authors.initials: R
authors.lastname: Mas
authors.forename: Roberto
authors.initials: R
authors.lastname: Menendez
authors.forename: Rosa M
authors.initials: RM
authors.lastname: Gonzalez
authors.forename: Ambar
authors.initials: A
authors.lastname: Oyarzabal
authors.forename: Sonia
authors.initials: S
authors.lastname: Jimenez
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D028023:Arecaceae; D004305:Dose-Response Relationship, Drug; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; C504503:D 004 extract; D010936:Plant Extracts; D008315:Malondialdehyde