Daily fluctuation of cortisol in the saliva and serum of healthy persons.
The objective of this study was to evaluate cortisol in the saliva and serum of healthy persons and its daily fluctuations by using immunochemical method on a autoanalyzer. Biological samples: Serum from 14 healthy persons and saliva from 18 healthy persons were taken two times at 8 a.m. and at 4 p.m. Immunochemical assay: The principle of this method is the competitive binding of cortisol present in the analyzed sample and cortisol marked with peroxides on binding parts with specific antibodies.


METHODS
Student t-test. Cortisol in saliva in the morning: 21,2 +/- 16,2 nmol/l and in the afternoon 12,7 +/- 8,1 nmol/l. Cortisol in serum in the morning: 459, 6 +/- 235,2 nmol/l , and in the afternoon 340,5 +/- 207,5 nmol/l. The concentrations of cortisol in saliva are lower than in serum. Cortisol in the serum in the morning is about twenty times higher than cortisol in the saliva at the same time. Cortisol in the serum at afternoon is about twenty-seven times higher than cortisol in the saliva. Individual variabilities of cortisol in the saliva and serum were found during the day.
Ljubijankić
Nevzeta
N
Faculty of Science, University of Sarajevo, Sarajevo, Bosnia and Herzegovina.
Popović-Javorić
Ranka
R
Sćeta
Sabiha
S
Sapcanin
Aida
A
Tahirović
Ismet
I
Sofić
Emin
E
D001331:Automation; D001826:Body Fluids; D002784:Cholesterol / Q000737:chemistry; D002940:Circadian Rhythm; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D006854:Hydrocortisone / Q000097:blood* / Q000737:chemistry / Q000378:metabolism*; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008956:Models, Chemical; D010507:Periodicity; D012463:Saliva / Q000378:metabolism*; D012737:Sex Factors; D013997:Time Factors
D002784:Cholesterol; D006854:Hydrocortisone