Identification and characterization of a splice variant of the catalytic domain of mouse NTE-related esterase.
Patatin-domain containing proteins constitute a large family of enzymes including known lipases that hydrolyze triglycerides, diglycerides, and phospholipids, some of which still remain to be characterized. One of those is NTE-related esterase (NRE), which exhibits sequence and domain homology to neuropathy target esterase (NTE). A splice variant of the catalytic domain of mouse NRE (mNRECV) was identified in multiple adult tissues, including brain, kidney, liver and testis. Genomic organization showed that mNRECV gene lacked the 22nd exon of mouse NRE and the 14th exon termination site of mNRECV was behind of 5 bp with the comparison of the 34th exon of mNRE gene. Over-expression of mNREC and mNRECV in mammalian cells showed that they had similar phenyl valerate esterase activities, but different from human NTE esterase domain. Subcellular distribution of an enhanced green fluorescent protein-mNRECV fusion protein was mainly observed to colocalize with endoplasmic reticulum in the juxtanuclear area and a little in cytoplasm. Moreover, autophagy/lysosome pathway was found to be involved in the degradation of mNRECV protein by inhibition and induction of autophagy, as well as co-location of mNRECV-EGFP with lysosomes. The high identity between mNRECV and mNREC suggested that mouse NRE has similar characteristics.
Chang
Ping-An
PA
Key Laboratory of Molecular Biology, College of Bio-information, Chongqing University of Posts and Telecommunications, Chongqing 400065, PR China. changpingan@yahoo.com.cn
Long
Ding-Xin
DX
Sun
Quan
Q
Wang
Qi
Q
Bu
You-Quan
YQ
Wu
Yi-Jun
YJ
D000595:Amino Acid Sequence; D000818:Animals; D001343:Autophagy; D001483:Base Sequence; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry* / Q000378:metabolism; D020134:Catalytic Domain; D002460:Cell Line; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms; D014018:Tissue Distribution; D014162:Transfection
D002265:Carboxylic Ester Hydrolases; D020033:Protein Isoforms; C530968:Pnpla7 protein, mouse; C021859:neurotoxic esterase