title: An 8-hour system for Salmonella detection with immunomagnetic separation and homogeneous time-resolved fluorescence PCR.
abstract: We describe a system consisting of rapid sample enrichment and homogeneous end-point PCR analysis that enables the detection of Salmonella in various food matrices in 8 h. Sample preparation starts with 6 h enrichment step in supplemented broth, after which Salmonella cells are collected with immunomagnetic particles. The particles are washed and dispensed to ready-to-use PCR reaction vessels, which contain dried assay-specific reagents and an internal amplification control. PCR is performed with a novel instrument platform utilising the sensitive label technology of time-resolved fluorometry. Qualitative assay results are automatically interpreted and available in 45 min after sample addition. The overall accuracy, sensitivity and specificity of the Magda CA Salmonella system were 99.1%, 98.4% and 100.0%, respectively, based on the evaluation of 107 samples (beef, pork, poultry and ready-to-eat meals) artificially contaminated with sub-lethally injured Salmonella cells.
authors.affiliation: Abacus Diagnostica Ltd., Tykistökatu 4D, FI-20520 Turku, Finland. virve.hagren@abacusdiagnostica.com
authors.forename: Virve
authors.initials: V
authors.lastname: Hagren
authors.forename: Piia
authors.initials: P
authors.lastname: von Lode
authors.forename: Anniina
authors.initials: A
authors.lastname: Syrjälä
authors.forename: Teemu
authors.initials: T
authors.lastname: Korpimäki
authors.forename: Mika
authors.initials: M
authors.lastname: Tuomola
authors.forename: Otto
authors.initials: O
authors.lastname: Kauko
authors.forename: Jussi
authors.initials: J
authors.lastname: Nurmi
mesh_terms: D001331:Automation; D005453:Fluorescence; D005470:Fluorometry / Q000379:methods* / Q000592:standards; D005506:Food Contamination / Q000032:analysis*; D005784:Gene Amplification; D018189:Immunomagnetic Separation / Q000379:methods* / Q000592:standards; D016133:Polymerase Chain Reaction / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D012475:Salmonella / Q000145:classification / Q000276:immunology / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D013045:Species Specificity; D013997:Time Factors