title: Peptides derived from a soluble molecule of the human leukocyte antigen (HLA) class I cause apoptosis in gastric cancer cell lines.
abstract: We have reported that the levels of the soluble molecule of the human leukocyte antigen class I (sHLA-I) in patients with advanced gastric cancer were significantly lower than those in patients with cancer in the early stages. However, the effect of sHLA-I on gastric cancer cells has not been elucidated. Using human gastric cancer cell lines, MKN28, MKN45, and MKN74, we evaluated the effects of sHLA-I on cell growth, DNA synthesis, and apoptosis induction. Three types of synthesized peptides derived from HLA-I were also examined for their capacity to induce apoptosis. sHLA-I and a synthesized peptide, nos. 220-232 of the alpha3 domain of HLA-B7, caused cell growth inhibition by inducing apoptosis in human gastric cancer cells. This peptide also inhibited the in vivo growth of cancer dissemination caused by an intraperitoneal injection of MKN45 into severe combined immunodeficient mice. In conclusion, sHLA-I and the peptides derived from HLA-I cause apoptosis in human gastric cancer cell lines.
authors.affiliation: Department of General Surgical Science (Surgery I), Gunma University Graduate School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma, 371-8511, Japan. tshimura@showa.gunma-u.ac.jp
authors.forename: Tatsuo
authors.initials: T
authors.lastname: Shimura
authors.forename: Taketoshi
authors.initials: T
authors.lastname: Suehiro
authors.forename: Hideki
authors.initials: H
authors.lastname: Suzuki
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Mochida
authors.forename: Koji
authors.initials: K
authors.lastname: Okada
authors.forename: Erito
authors.initials: E
authors.lastname: Mochiki
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kuwano
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D006680:HLA Antigens / Q000494:pharmacology*; D015395:Histocompatibility Antigens Class I / Q000494:pharmacology; D006801:Humans; D007694:Killer Cells, Natural / Q000187:drug effects; D051379:Mice; D008819:Mice, Nude; D010455:Peptides / Q000494:pharmacology*; D013274:Stomach Neoplasms / Q000473:pathology*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects; D023041:Xenograft Model Antitumor Assays
chemical_list: D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D010455:Peptides