[Polymorphisms of chicken apoA5 gene and association with carcass traits of chickens].
Seven single nucleotide polymorphisms (SNPs) were identified by PCR-SSCP and sequencing in the chicken apoA5 gene in F2 chickens from an experimental cross of White Plymouth Rock x Silkies. One SNP(C-169T) located on the 5'-regulatory region, another two in the second exon were transitions of C to T (600) and T to C (635). Four SNPs in the third exon were found, which were C841G, C914T, C1142G, C1394T. The association of the polymorphisms with carcass traits was investigated. The most significant results were yielded from primer apoA3F/R: the abdominal fat weight of CC chickens were significantly higher than that of AA, AB, AC, BB and BC chickens (P<0.05); AC chickens had lower liver weight than that of AA, AB, BB, BC and CC (P<0.05); BC chickens had lower heart weight than that of BB (P<0.05).
Yao
Jun-Feng
JF
College of Animal Science and Technology, China Agricultural University, Beijing 100094, China. jfyao@cau.edu.cn
Zhang
Ying
Y
Wu
Gui-Qin
GQ
Zheng
Jiang-Xia
JX
Deng
Xue-Mei
XM
Yang
Ning
N
D050153:Abdominal Fat / Q000378:metabolism; D000818:Animals; D001054:Apolipoproteins A / Q000235:genetics*; D002645:Chickens / Q000235:genetics*; D009929:Organ Size / Q000235:genetics; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics
D001054:Apolipoproteins A