Encapsulation of all-trans retinoic acid into wheat ceramides micro vesicles enhanced influence on collagenase and tissue inhibitor of metalloproteinase-1 production by human skin fibroblasts.
The influence of all-trans retinoic acid (RA), either in its free or encapsulated form into wheat ceramides (CER), on the production of collagenase (MMP-1), and tissue inhibitor of metalloproteinase (TIMP-1) by human skin fibroblasts (HSF) at early and late stages of their proliferative life span (PLS) was examined. The level of MMP-1 was elevated and that of TIMP-1 decreased in late as compared to early passage cells. All-trans retinoic acid significantly decreased and increased the secretions of MMP-1 and TIMP-1 respectively, in a dose-dependent manner, from 10-7 m to 10-5 m. Entrapment of RA into CER vesicles potentiated its effect on MMP-1 and TIMP-1 secretions by HSF, independently of cell passages. The extent of variations obtained on MMP-1 and TIMP-1 levels, when HSF culture medium was supplemented with 10-5 m RA, could be obtained using a 100-fold lower concentration of RA encapsulated into CER vesicles. CER had no effect on TIMP-1 and MMP production by HSF in culture, and simultaneous addition of CER and RA did not potentiate the effects of RA alone, indicating that formation of RA-CER liposomes was responsible for the enhancing effects. The rate of internalization of RA into HSF was increased when used in its CER encapsulated form. Therefore, the use of retinoid within CER potentiates its beneficial influence on the MMP-1:TIMP-1 imbalance with fibroblastageing.
Bizot-Foulon
V
V
Extraction, Purification, Innovation (EPI)France Montleven France.
Rousset
G
G
Buisson
N
N
Hornebeck
W
W