Effect of oxidised regenerated cellulose/collagen matrix on proteases in wound exudate of patients with chronic venous ulceration.
Oxidised regenerated cellulose/collagen matrix (ORC/collagen matrix) modifies wound microenvironments by binding and inactivating excess levels of proteases such as elastase, plasmin and gelatinases in wound exudates. To compare levels of the gelatinases matrix metalloproteinase 2 (MMP-2), elastase and plasmin in wound exudates collected from chronic venous insufficiency patients with venous leg ulcers treated with either an ORC/collagen matrix or a standard control therapy. During a 12-week treatment period, wound exudate samples were obtained from a control group of 10 patients treated with a hydrocolloid dressing and a treatment group of 17 patients treated with a combination of ORC/collagen matrix and hydrocolloid dressing. On admission and days 5, 14 and every subsequent 14th day, ulcers were photographed to determine healing rate and changes in ulcer appearance, and MMP-2 concentration and the gelatinase, elastase and plasmin activities were analysed from wound exudates. The patients treated with ORC/collagen matrix showed a significant decrease in elastase, plasmin and gelastinase activity as compared with the control group, with no significant difference in the MMP-2 concentrations between the two groups. The results show a significant and immediate reduction in protease activity in wound exudates from venous leg ulcers treated with ORC/collagen.
Smeets
Ralf
R
Interdisciplinary Centre of Clinical Research BioMAT, University Hospital Aachen, D-52074 Aachen, Germany. rasmeets@ukaachen.de
Ulrich
Dietmar
D
Unglaub
Frank
F
Wöltje
Michael
M
Pallua
Norbert
N
D000368:Aged; D001458:Bandages; D002483:Cellulose, Oxidized; D002908:Chronic Disease; D003094:Collagen; D005122:Exudates and Transudates / Q000201:enzymology*; D005260:Female; D006490:Hemostatics; D006801:Humans; D008297:Male; D008875:Middle Aged; D010447:Peptide Hydrolases / Q000378:metabolism*; D016896:Treatment Outcome; D014647:Varicose Ulcer / Q000201:enzymology* / Q000628:therapy; D014945:Wound Healing / Q000502:physiology
D002483:Cellulose, Oxidized; D006490:Hemostatics; D003094:Collagen; D010447:Peptide Hydrolases