title: Cloning, expression and evaluation of the efficacy of a recombinant Eimeria tenella sporozoite antigen in birds.
abstract: Eimeria infection in poultry is of significant economic interest worldwide. Development of a cost-effective sub-unit vaccine that provides cross-protection may help reduce loss in poultry industry. One approach explored by many investigators is to block the parasite invasion into gut epithelium. Use of microneme proteins to prevent parasite invasion is one of the most straightforward approaches in developing a preventive vaccine. Here we describe cloning and expression of microneme-1 protein of Eimeria tenella, obtained from an outbreak sample from India. We have evaluated the ability of the recombinant protein to elicit both cell mediated immune (CMI) and humoral immune responses. We also evaluated the efficacy of the recombinant protein in protecting against a homologous challenge. Our data indicate recombinant EtMIC1 is able to impart partial protection against homologous challenge in chicken. Inclusion of more invasion proteins may improve the efficacy of prophylactic vaccine against Coccidiosis.
authors.affiliation: Indian Immunologicals Limited, Research and Development, Gachibowli, Hyderabad 500032, Andhra Pradesh, India.
authors.forename: B Mohana
authors.initials: BM
authors.lastname: Subramanian
authors.forename: R
authors.initials: R
authors.lastname: Sriraman
authors.forename: N Hanumantha
authors.initials: NH
authors.lastname: Rao
authors.forename: J
authors.initials: J
authors.lastname: Raghul
authors.forename: D
authors.initials: D
authors.lastname: Thiagarajan
authors.forename: V A
authors.initials: VA
authors.lastname: Srinivasan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000235:genetics* / Q000276:immunology*; D001715:Bird Diseases / Q000276:immunology / Q000517:prevention & control*; D002478:Cells, Cultured; D002645:Chickens; D003001:Cloning, Molecular; D003048:Coccidiosis / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary*; D016786:Eimeria tenella / Q000235:genetics / Q000302:isolation & purification; D015870:Gene Expression; D007194:India; D007371:Interferon-gamma / Q000096:biosynthesis; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D008969:Molecular Sequence Data; D011529:Protozoan Infections, Animal / Q000276:immunology / Q000517:prevention & control*; D016052:Protozoan Vaccines / Q000276:immunology*; D017422:Sequence Analysis, DNA; D013154:Spleen / Q000276:immunology
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D016052:Protozoan Vaccines; C061194:Eimeria tenella sporozoite antigen; D007371:Interferon-gamma