Monocyte activation in on-pump versus off-pump coronary artery bypass surgery.
OBJECTIVE
Monocyte activation plays a key role in amplifying both inflammatory and coagulopathic sequelae in patients undergoing on-pump coronary artery bypass graft (CABG) surgery. Off-pump CABG diminishes, but does not eliminate, the systemic inflammatory response and its influence on monocyte activation remains unclear. This study was performed to determine if off-pump CABG suppresses all features of monocyte activation.


METHODS
Prospective, controlled, clinical study.


METHODS
University-affiliated veterans affairs hospital and laboratory.


METHODS
Twenty-two patients scheduled to undergo primary CABG surgery (11 on-pump and 11 off-pump).


METHODS
On-pump and off-pump CABG surgery was performed via median sternotomy. Anticoagulation and heparin reversal were identical. Moderate hypothermia (28 degrees-30 degrees C) was used for on-pump CABG surgery, whereas temperature was maintained above 35.5 degrees C for off-pump CABG. No antifibrinolytic agents were used.


RESULTS
Perioperative monocyte changes were assessed by using cellular (CD11b, monocyte-platelet conjugates) and secreted markers (plasma IL-6, IL-8, and IL-10) measured at 6 time points before, during, and after CABG surgery. Off-pump CABG surgery completely blocked the increases in monocyte CD11b expression (p < 0.001) and monocyte-platelet conjugate formation (p < 0.001) observed in the on-pump group. In contrast, plasma interleukin levels were significantly elevated in both groups, although off-pump CABG surgery resulted in lower levels (p < 0.001) and a delayed time course.


CONCLUSIONS
Off-pump CABG surgery attenuates monocyte secreted cytokines and completely suppresses activation-dependent monocyte cell-surface changes (CD11b, monocyte-platelet conjugate formation). Whether these pathophysiologic differences in monocyte activation translate into a reduction in adverse events after CABG surgery warrants a larger, randomized, outcomes study.
Greilich
Philip E
PE
Department of Anesthesiology and Pain Management, UT Southwestern Medical Center, Dallas, TX 75390-9202, USA. philip.greilich@utsouthwestern.edu
Brouse
Chad F
CF
Rinder
Henry M
HM
Jessen
Michael E
ME
Rinder
Christine S
CS
Eberhart
Robert C
RC
Whitten
Charles W
CW
Smith
Brian R
BR
D000368:Aged; D000369:Aged, 80 and over; D001026:Coronary Artery Bypass / Q000379:methods; D047549:Coronary Artery Bypass, Off-Pump / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D019990:Perioperative Care / Q000379:methods; D011446:Prospective Studies