Preparation of Cu2+-loaded porous chitosan particles for immunoglobulin G adsorption.
Chitosan porous particles were prepared using a precipitation technique. The porous particles could bind Cu(2+), from which Cu(2+)-loaded porous particles were prepared. The Cu(2+)-loaded porous chitosan particles could remove immunoglobulin (Ig) G more selectively than albumin, IgA and IgM from aqueous solutions and human plasma. The effect of the volume ratio of plasma to particles on protein adsorption was studied, with the results indicating that a volume ratio of 3:1 might be a good choice for clinical use. The particles could be easily incorporated into a column. When human plasma was applied to the particle column, higher removal efficiency was obtained. These results suggested that Cu(2+)-loaded porous particles may be a potentially good sorbent for IgG removal from plasma.
Yue
Rongzheng
R
College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, China.
Yue
Rong-Zheng
RZ
Liu
Ting
T
Su
Bai-Hai
BH
Fu
Ping
P
Zhao
Fan
F
Meng
Wentong
W
Zhao
Fan
F
Zhao
Changsheng
C
Zhao
Chang-Sheng
CS
D001798:Blood Proteins; D048271:Chitosan; D003300:Copper; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007163:Immunosorbent Techniques; D010316:Particle Size; D016062:Porosity
D001798:Blood Proteins; D007074:Immunoglobulin G; D003300:Copper; D048271:Chitosan