title: RNAi-mediated crop protection against insects.
abstract: Downregulation of the expression of specific genes through RNA interference (RNAi), has been widely used for genetic research in insects. The method has relied on the injection of double-stranded RNA (dsRNA), which is not possible for practical applications in crop protection. By contrast, specific suppression of gene expression in nematodes is possible through feeding with dsRNA. This approach was thought to be unfeasible in insects, but recent results have shown that dsRNA fed as a diet component can be effective in downregulating targeted genes. More significantly, expression of dsRNA directed against suitable insect target genes in transgenic plants has been shown to give protection against pests, opening the way for a new generation of insect-resistant crops.
authors.affiliation: School of Biological and Biomedical Sciences, Durham University, South Road, Durham DH1 3LE, UK.
authors.forename: Daniel R G
authors.initials: DR
authors.lastname: Price
authors.forename: John A
authors.initials: JA
authors.lastname: Gatehouse
mesh_terms: D000818:Animals; D018556:Crops, Agricultural / Q000235:genetics*; D004330:Drosophila / Q000235:genetics; D007300:Insect Control / Q000379:methods*; D007313:Insecta / Q000235:genetics*; D010572:Pest Control, Biological / Q000379:methods*; D030821:Plants, Genetically Modified; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000378:metabolism
chemical_list: D012330:RNA, Double-Stranded