title: Odontogenic ameloblast-associated protein nature of the amyloid found in calcifying epithelial odontogenic tumors and unerupted tooth follicles.
abstract: We have previously reported that the amyloid found in three patients with calcifying epithelial odontogenic tumors (CEOT) was composed of N-terminal fragments of a putative 153-residue protein specified by a gene designated FLJ20513 now known to represent exons 5 through 10 of the odontogenic ameloblast-associated protein (ODAM) locus that encodes a 279-residue polypeptide. Confirmation of the amyloidogenic potential of ODAM has resulted from analyses of four other cases where we found, in addition, a 74-residue segment specified by exon 4. Through preparation of ODAM-related synthetic peptides, it was possible to localize the fibril-forming region of this molecule, as well as generate a monoclonal antibody that reacted specifically with the amyloid associated with CEOT. Notably, we also detected green birefringent congophilic material in unerupted tooth follicles - a precursor of CEOT - and demonstrated through immunologic and chemical analyses the ODAM nature of the deposits. Our studies have provided further evidence for this unique form of odontogenic amyloid that we provisionally designate "AODAM".
authors.affiliation: Human Immunology and Cancer Program, Department of Medicine, University of Tennessee Graduate School of Medicine, Knoxville, Tennessee 37920, USA.
authors.forename: Charles L
authors.initials: CL
authors.lastname: Murphy
authors.forename: Daniel P
authors.initials: DP
authors.lastname: Kestler
authors.forename: James S
authors.initials: JS
authors.lastname: Foster
authors.forename: Shuching
authors.initials: S
authors.lastname: Wang
authors.forename: Sallie D
authors.initials: SD
authors.lastname: Macy
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Kennel
authors.forename: Eric R
authors.initials: ER
authors.lastname: Carlson
authors.forename: John
authors.initials: J
authors.lastname: Hudson
authors.forename: Deborah T
authors.initials: DT
authors.lastname: Weiss
authors.forename: Alan
authors.initials: A
authors.lastname: Solomon
mesh_terms: D000565:Ameloblasts / Q000378:metabolism*; D000595:Amino Acid Sequence; D000682:Amyloid / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D000686:Amyloidosis / Q000209:etiology / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D007573:Jaw Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008969:Molecular Sequence Data; D009805:Odontogenesis / Q000502:physiology*; D009808:Odontogenic Tumors / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D017386:Sequence Homology, Amino Acid; D014097:Tooth, Unerupted / Q000378:metabolism*
chemical_list: D000682:Amyloid