title: IL-1 and its role in rat carrageenan pleurisy.
abstract: The carrageenan pleurisy model, which is characterized by cellular influx and oedema, has been used to examine the effects of anti-inflammatory compounds such as naproxen. Interleukin-1alpha and beta (IL-1) are known to be pro-inflammatory mediators, and their roles in this model are unknown. Intrapleural injection of 1% viscarin carrageenan or saline was administered to male Lewis rats. Four to 24 h later, cell counts, fluid volumes and IL-1beta levels (measured by ELISA) were determined in the pleural cavity. Serum corticosterone levels were measured only at 4 h. Significant increases in IL-1beta levels precede cell influx suggesting IL-1beta plays a role in the maintenance of cell accumulation in the pleural cavity. None of the drugs tested, including the IL-1 receptor antagonist, maintained pleural cell influx and IL-1beta levels at control levels. When human IL-1alpha or beta or rat IL-1beta were injected individually into the pleural cavity, none of these cytokines were pro-inflammatory, as measured by increased cell influx and fluid extravasation. These results suggest that although IL-1beta levels increase in the pleural cavity in response to carrageenan, IL-1 per se is not the initiator of the pro-inflammatory events of cell influx and oedema in this model.
authors.affiliation: The DuPont Merck Pharmaceutical Company Inflammatory Diseases Research Experimental Station E400/4223 Wilmington DE 19898-0400 USA.
authors.forename: R
authors.initials: R
authors.lastname: Goodman
authors.forename: L R
authors.initials: LR
authors.lastname: Mantegna
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authors.initials: CL
authors.lastname: McAilister
authors.forename: E
authors.initials: E
authors.lastname: Bruin
authors.forename: R L
authors.initials: RL
authors.lastname: Dowling
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authors.lastname: George
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authors.lastname: Feeser
authors.forename: B
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authors.lastname: Freimark
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authors.lastname: Lischwe
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authors.lastname: Pick
authors.forename: R R
authors.initials: RR
authors.lastname: Harris
authors.forename: J S
authors.initials: JS
authors.lastname: Kerr