Correlation between autofluorescence intensity and tumor area in mice bearing renal cell carcinoma.
Protoporphyrin IX (PpIX) is a porphyrin derivative that is accumulated in cancerous tissue in consequence of the tumor-specific metabolic alterations. The aim of this study was to evaluate the accumulation of PpIX in mice bearing renal cell carcinoma by spectroscopy analysis. A total of 24 male Balb/c mice, 6 weeks old, were divided into six groups: Normal (without inoculation of tumor cells) and 4, 8, 13, 16, and 20 days after inoculation of tumor cells. The orthotopic tumor model of renal cancer was used. Murine renal cell carcinoma (Renca cells) were inoculated into the subcapsular space of the kidney. Normal and tumor-bearing kidneys in different progression stages were removed and analyzed by ex-vivo spectroscopy and by microscopy, for tumor histometric analysis. Emission spectra were obtained by exciting the samples at 405 nm. Significant differences between normal and tumor-bearing kidneys in autofluorescence shape occurred in the 600-700 nm spectral region. A good correlation was found between emission band intensity at 635 nm and the tumor area.
Bellini
Maria Helena
MH
Disciplina de Nefrologia, Departamento de Medicina, UNIFESP, São Paulo, SP, Brazil. mhmarumo@terra.com.br
Coutinho
Enia Lúcia
EL
Courrol
Lilia Coronato
LC
Rodrigues de Oliveira Silva
Flávia
F
Vieira Júnior
Nilson Dias
ND
Schor
Nestor
N
D000818:Animals; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D018450:Disease Progression; D005453:Fluorescence; D007668:Kidney / Q000473:pathology*; D007680:Kidney Neoplasms / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008856:Microscopy, Fluorescence / Q000379:methods; D011524:Protoporphyrins / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods; D014407:Tumor Cells, Cultured
D011524:Protoporphyrins; C028025:protoporphyrin IX