title: Detection of circulating trophoblast particles in peripheral maternal blood in preeclampsia complicated pregnancies.
abstract: OBJECTIVE
  Data on trophoblast particles in peripheral maternal circulation are rare. Applying a new enrichment technique, Oncoquick, we evaluated the number of trophoblast particles in maternal blood in preeclamptic and non-preeclamptic pregnancies.
  
  
  METHODS
  After isolation of trophoblast particles using Oncoquick, trophoblast particles were detected by immunocytochemistry, applying a monoclonal pan cytokeratin (CK) antibody. Fetal origin of the detected particles was verified by XY- fish analysis in women delivering male infants.
  
  
  RESULTS
  Trophoblast particles were identified in 25/52 (48%) of preeclamptic and 1/20 (5%) of non-preeclamptic pregnancies.
  
  
  CONCLUSIONS
  The number of trophoblast particles in peripheral maternal blood of preeclamptic pregnancies was significantly higher than in non-preeclamptic pregnancies.
authors.affiliation: Department of Gynecology and Obstetrics, University of Duisburg-Essen, Essen, Germany. markus.schmidt@uk-essen.de
authors.forename: Markus
authors.initials: M
authors.lastname: Schmidt
authors.forename: Barbara
authors.initials: B
authors.lastname: Hoffmann
authors.forename: Dietrich
authors.initials: D
authors.lastname: Beelen
authors.forename: Alexandra
authors.initials: A
authors.lastname: Gellhaus
authors.forename: Elke
authors.initials: E
authors.lastname: Winterhager
authors.forename: Rainer
authors.initials: R
authors.lastname: Kimmig
authors.forename: Sabine
authors.initials: S
authors.lastname: Kasimir-Bauer
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007231:Infant, Newborn; D008297:Male; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis*; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology*