title: Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: application to Prnp.
abstract: Spatial and temporal control of ovine prion protein (Prnp) gene expression was achieved in mice using two transgenes: a Prnp minigene with tet-operator sequences inserted 5' to exon 1 and a mouse neurofilament genomic clone carrying the chimeric-repressor TRSID cDNA. In bi-transgenic mice, ovine PrP(C) expression could be reversibly controlled in neuronal cells by doxycycline treatment whereas it remains constant in other cell types. Overall, this model opens opportunities to assess the involvement of cell types in prion diseases and PrP physiological function. It demonstrates the potentiality of the TRSID-silencer to precisely control temporal and spatial gene expression in vivo.
authors.affiliation: Laboratoire de Génétique Biochimique et de Cytogénétique, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France.
authors.forename: Micaela
authors.initials: M
authors.lastname: Gallozzi
authors.forename: Vincent
authors.initials: V
authors.lastname: Béringue
authors.forename: Pauline
authors.initials: P
authors.lastname: Decaunes
authors.forename: Annick
authors.initials: A
authors.lastname: Le Dur
authors.forename: Karine
authors.initials: K
authors.lastname: Le Roux
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Tilly
authors.forename: Sandrine
authors.initials: S
authors.lastname: Le Guillou
authors.forename: Laetitia
authors.initials: L
authors.lastname: Herzog
authors.forename: Coralie
authors.initials: C
authors.lastname: Peyre
authors.forename: Aline
authors.initials: A
authors.lastname: Ladroue
authors.forename: Jérôme
authors.initials: J
authors.lastname: Chapuis
authors.forename: Marthe
authors.initials: M
authors.lastname: Vilotte
authors.forename: Bruno
authors.initials: B
authors.lastname: Passet
authors.forename: José
authors.initials: J
authors.lastname: Costa
authors.forename: Nathalie
authors.initials: N
authors.lastname: Chenais
authors.forename: Fabienne
authors.initials: F
authors.lastname: Le Provost
authors.forename: Hubert
authors.initials: H
authors.lastname: Laude
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Vilotte
mesh_terms: D000818:Animals; D015536:Down-Regulation; D015870:Gene Expression; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism; D018621:PrPC Proteins / Q000235:genetics*; D012756:Sheep / Q000235:genetics*; D037941:Silencer Elements, Transcriptional; D019076:Transgenes
chemical_list: D018621:PrPC Proteins