[Isolation and characterization of a low molecular weight glutenin gene from Taenitherum Nevski].
More and more low-molecular-weight glutenin(LMW glutenin) genes were isolated and characterized from hexaploid wheat (Triticum aestivum L.). However, few homologous genes were obtained from its relative species, which limited our understanding of the relationships among them. Therefore, it is necessary to isolate LMW glutenin homologous genes from wheat wild relative species. Using a pair of specific oligonucleotide PCR primers for Taenitherum genomic DNA, a LMW glutenin gene sequence, with nucleotide sequence in 1 035 bp and deduced amino acid sequence with 343 amino acid residues, was obtained. This sequence was a typical LMW glutenin sequence and characterized by a signal peptide of 21 amino acid residues, a N-terminal conservative domain of 13 amino acid residues, a repetitive domain of short peptide, and a C-terminal conservative domain. Sequence alignment showed the main differences and the relationships between LMW glutenin genes from wheat and Taenitherum. The results presented here give a reference to isolate LMW glutenin gene from Taenitherum, as well as other wheat wild relatives.
Zeng
Jie
J
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan 611830, China. chuannong58@sina.com
Dai
Shou-Fen
SF
Zheng
You-Liang
YL
Liu
Deng-Cai
DC
Wei
Yu-Ming
YM
Yan
Ze-Hong
ZH
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular / Q000379:methods*; D005983:Glutens / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction; D016415:Sequence Alignment; D014908:Triticum / Q000235:genetics*
D010940:Plant Proteins; D005983:Glutens; C037302:glutenin