Simultaneous determination of tetracylines and quinolones antibiotics in egg by ultra-performance liquid chromatography-electrospray tandem mass spectrometry.
We report here an ultra-performance liquid chromatography coupled with tandem mass spectrometric (MS/MS) method for the simultaneous quantitation of multiclass veterinary drugs in egg. The analysis of the target compounds, including 7 tetracyclines and 4 types of quinolones, may be accomplished in 15 min of total run time. The egg was extracted with ethylenediaminetetraacetic acid-Mcllvaine buffer solution and further purified using a polymer-based Oasis HLB solid-phase extraction cartridge. A C18 column was used to separate the analytes followed by MS/MS using an electrospray ion source. The overall average recoveries of the analytes based on matrix-fortified calibration ranged from 71 to 112% with acceptable relative standard deviations of <20% for 6 trials. For all of the target compounds, the limits of quantitation ranged between 0.02 and 4.29 microg/kg. The proposed method is sufficiently sensitive and highly selective.
Jia
Xiaofei
X
Beijing Center for Disease Control and Prevention, Beijing 100013, People's Republic of China.
Shao
Bing
B
Wu
Yongning
Y
Yang
Yi
Y
Zhang
Jing
J
D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004531:Eggs / Q000032:analysis*; D015363:Quinolones / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013754:Tetracyclines / Q000032:analysis*
D000900:Anti-Bacterial Agents; D015363:Quinolones; D013754:Tetracyclines