H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens.
Using plasmid-based reverse genetics, we generated a molecularly altered virus, H5N1/PR8-5B19, containing modified HA and NA genes from A/Goose/Guangdong/1/96 (GS/GD/1/96). In the H5N1/PR8-5B19 virus, the HA cleavage site was modified to resemble that of low-pathogenic avian strains and a portion of the NA stalk region was replaced by the immunodominant 5B19 epitope of the S2 glycoprotein of murine hepatitis virus (MHV). H5N1/PR8-5B19 is not lethal to embryonated eggs or chickens. Chickens immunized with the H5N1/PR8-5B19 inactivated vaccine produced high levels of HI antibody and a measurable antibody response against the MHV 5B19 epitope, and were fully protected against subsequent challenge with different highly pathogenic H5N1 avian influenza viruses. H5N1/PR8-5B19 is therefore an attractive marker vaccine candidate, eliciting a strong, protective antibody response and enabling serological discrimination between vaccinated and wild-type virus-infected chickens.
Li
Chengjun
C
Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin, Heilongjiang 150001, People's Republic of China.
Ping
Jihui
J
Jing
Bo
B
Deng
Guohua
G
Jiang
Yongping
Y
Li
Yanbing
Y
Tian
Guobin
G
Yu
Kangzhen
K
Bu
Zhigao
Z
Chen
Hualan
H
D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D000917:Antibody Formation; D002645:Chickens / Q000276:immunology* / Q000821:virology*; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D005585:Influenza in Birds / Q000517:prevention & control*; D006517:Murine hepatitis virus / Q000235:genetics / Q000276:immunology; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA; D015164:Vaccines, Inactivated / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
D000914:Antibodies, Viral; D006023:Glycoproteins; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D016056:Immunodominant Epitopes; D007252:Influenza Vaccines; D019444:Vaccines, DNA; D015164:Vaccines, Inactivated; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase