The regulatory CD4+CD25+ T cells have a limited role on pathogenesis of infection with Trypanosoma cruzi.
Recent reports have established an important role of CD4+CD25+ T cells in the immune regulation of infectious diseases, autoimmune disorders and cancer. In the present work, we investigated whether these cells had a regulatory role during Trypanosoma cruzi infection, using the Colombian strain. Inactivation of CD4+CD25+ cells in vivo conferred mice slightly more resistant to infection with the Colombian strain of T. cruzi, as evidenced by lower parasitemia and mortality rates. The augmented resistance to infection with Colombian strain did correlate with increased activation of effector CD4 cells. It was antibody-independent, since no difference in levels of IgM, IgG, IgG1 and IgG2a(b) recognizing T. cruzi antigens was observed throughout the infection of CD25-inactivated and control mice. Regarding pathogenesis, inflammatory infiltrate and frequency of CD4 and CD8 T cells or macrophages in the cardiac tissue was similar in both groups. Together, our data indicate that CD4+CD25+ cells have a limited role on host resistance during early T. cruzi infection. Despite exhaustive investigation, we did not observe any role for these regulatory cells in the pathogenesis of experimental chronic Chagas' disease.
Sales
Policarpo A
PA
Hospital Santa Casa de Misericórdia de Belo Horizonte, Núcleo de Pós-Graduação e Pesquisa, Belo Horizonte, MG, Brazil.
Golgher
Denise
D
Oliveira
Roberta V
RV
Vieira
Valeska
V
Arantes
Rosa M E
RM
Lannes-Vieira
Joseli
J
Gazzinelli
Ricardo T
RT
D000818:Animals; D015704:CD4 Antigens / Q000032:analysis*; D014355:Chagas Disease / Q000276:immunology*; D053645:Interleukin-2 Receptor alpha Subunit / Q000032:analysis / Q000378:metabolism; D051379:Mice; D016176:T-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology*; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000472:pathogenicity*
D015704:CD4 Antigens; D053645:Interleukin-2 Receptor alpha Subunit