title: Duplication and divergence of zebrafish CRALBP genes uncovers novel role for RPE- and Muller-CRALBP in cone vision.
abstract: OBJECTIVE
  During vertebrate phototransduction 11-cis-retinal is isomerized to all-trans-retinal. Light sensitivity is restored by recombination of apo-opsin with 11-cis-retinal to regenerate visual pigments. The conversion of all-trans retinal back to 11-cis-retinal is known as the visual cycle. Within the retina, cellular retinal-binding protein (CRALBP) is abundantly expressed in the retinal pigment epithelium (RPE) and Müller glia. CRALBP expressed in the RPE is known to facilitate the rate of the rod visual cycle. Recent evidence suggests a role for Müller glia in an alternate cone visual cycle. In this study, the role of RPE- and Müller-CRALBP in cone vision was characterized.
  
  
  METHODS
  The CRALBP orthologues rlbp1a and rlbp1b were identified in zebrafish by bioinformatic methods. The spatial and developmental expression of rlbp1a and rlbp1b was determined by in situ hybridization and immunohistochemistry. Depletion of the expression of the corresponding Cralbp a and Cralbp b proteins was achieved by microinjection of antisense morpholinos. Visual function was analyzed in 5-day post fertilization (dpf) larvae using the optokinetic response assay.
  
  
  RESULTS
  The zebrafish genome contains two CRALBP ohnologues, rlbp1a and rlbp1b. These genes have functionally diverged, exhibiting differential expression at 5 dpf in RPE and Müller glia, respectively. Depletion of CRALBP in the RPE or Müller glia results in abnormal cone visual behavior.
  
  
  CONCLUSIONS
  The results suggest that cone photoreceptors incorporate 11-cis-retinoids derived from the rod and cone visual cycles into their visual pigments and that Müller-CRALBP participates in the cone visual cycle.
authors.affiliation: UCD Conway Institute and UCD School of Biomolecular and Biomedical Sciences, University College Dublin, Dublin, Ireland.
authors.forename: Ross
authors.initials: R
authors.lastname: Collery
authors.forename: Sarah
authors.initials: S
authors.lastname: McLoughlin
authors.forename: Victor
authors.initials: V
authors.lastname: Vendrell
authors.forename: Jennifer
authors.initials: J
authors.lastname: Finnegan
authors.forename: John W
authors.initials: JW
authors.lastname: Crabb
authors.forename: John C
authors.initials: JC
authors.lastname: Saari
authors.forename: Breandán N
authors.initials: BN
authors.lastname: Kennedy
mesh_terms: D020121:5' Untranslated Regions / Q000235:genetics; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics*; D002417:Cattle; D002645:Chickens; D020440:Gene Duplication; D018507:Gene Expression Regulation, Developmental; D014644:Genetic Variation; D016678:Genome; D017403:In Situ Hybridization; D010857:Pigment Epithelium of Eye / Q000502:physiology*; D017949:Retinal Cone Photoreceptor Cells / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D014982:Xenopus laevis; D015027:Zebrafish / Q000235:genetics* / Q000254:growth & development
chemical_list: C020932:11-cis-retinal-binding protein; D020121:5' Untranslated Regions; D002352:Carrier Proteins