title: Evaluation of quantitative anti-F1 IgG and anti-V IgG ELISAs for use as an in vitro-based potency assay of plague vaccine in mice.
abstract: Quantitative anti-F1 and anti-V IgG enzyme-linked immunosorbent assays (ELISAs) were developed to measure the serological response of female Swiss Webster mice after vaccination with the recombinant fusion protein, rF1-V, which is being developed as a plague vaccine. Several fundamental parameters of the ELISA were evaluated: specificity, precision, accuracy, and stability. Experimental results suggested that a potency assay based upon the serological response of female Swiss Webster mice, as measured by quantitative anti-F1 IgG and anti-V IgG ELISAs, might be used to evaluate the rF1-V fusion protein vaccine.
authors.affiliation: United States Army Medical Research Institute of Infectious Disease, Bacteriology Division, 1425 Porter Street, Fort Detrick, MD 21702-5011, USA. stephen.little@amedd.army.mil
authors.forename: S F
authors.initials: SF
authors.lastname: Little
authors.forename: W M
authors.initials: WM
authors.lastname: Webster
authors.forename: H
authors.initials: H
authors.lastname: Wilhelm
authors.forename: B
authors.initials: B
authors.lastname: Powell
authors.forename: J
authors.initials: J
authors.lastname: Enama
authors.forename: J J
authors.initials: JJ
authors.lastname: Adamovicz
mesh_terms: D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D010931:Plague Vaccine / Q000276:immunology* / Q000592:standards*; D012015:Reference Standards; D015203:Reproducibility of Results; D013379:Substrate Specificity
chemical_list: D007074:Immunoglobulin G; D010931:Plague Vaccine