title: [Serum proteomic variation study in patients with Crohn disease].
abstract: OBJECTIVE
  To search differentially expressed proteins in serum of patients with Crohn disease.
  
  
  METHODS
  Serum protein samples from 4 patients with Crohn disease and 8 healthy adults were recruited cross-labeled with variant CyDye, and then followed by two-dimensional differential in-gel electrophoresis (2-D DIGE), image analysis, and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).
  
  
  RESULTS
  The 2-D electrophoresis results were compared between the Crohn disease patients and the healthy adults. The spot 1058 expression in serum of Crohn disease patients increased by 1.68 folds as compared with healthy adults (P<0.05). The protein was identified as haptoglobin by mass spectrometry.
  
  
  CONCLUSIONS
  Up-regulating expression of haptoglobin in serum of Crohn disease patients may play a role in disequilibrium of immunity system.
authors.affiliation: Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Kang
authors.forename: Zu-Li
authors.initials: ZL
authors.lastname: Yang
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Long-Juan
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Shao-Jun
authors.initials: SJ
authors.lastname: Liu
authors.forename: Mei-Jin
authors.initials: MJ
authors.lastname: Huang
authors.forename: Ming-Tao
authors.initials: MT
authors.lastname: Li
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Wang
mesh_terms: D000328:Adult; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D003424:Crohn Disease / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001798:Blood Proteins; D006242:Haptoglobins