title: Effects of short deprivation and re-exposure intervals on the ethanol drinking behavior of selectively bred high alcohol-consuming rats.
abstract: Alcoholics generally display cycles of excessive ethanol intake, abstinence and relapse behavior. Using an animal model of relapse-like drinking, the alcohol deprivation effect (ADE), our laboratory has shown that repeated 2-week cycles of ethanol deprivation and re-exposure, following an initial 6-week access period, result in a robust ADE by alcohol-preferring (P) and high alcohol-drinking (HAD-1 and HAD-2) rats. These rat lines have been selectively bred to prefer a 10% ethanol solution over water. The present study examined whether P and HAD rats would display an ADE using much shorter ethanol deprivation and re-exposure intervals. Rats were given either continuous or periodic concurrent access to multiple concentrations (10%, 20%, and 30% [vol/vol]) of ethanol. The periodic protocol involved access to ethanol for 12 days followed by four cycles of 4 days of deprivation and 4 days of re-exposure to ethanol access. High-alcohol-drinking rats displayed a robust 24-h ADE upon first re-exposure (HAD-1: approximately 5 vs. 8g/kg/day; HAD-2: approximately 6 vs. 9g/kg/day, baseline vs. re-exposure), whereas P rats ( approximately 7 vs. 8g/kg/day) displayed a modest, nonsignificant, increase in 24-h intake. In a separate group of rats, ethanol intake and blood alcohol concentrations after the first hour of the fourth re-exposure cycle were HAD-1: 2.0g/kg and 97 mg%, HAD-2: 2.3g/kg and 73 mg%, and P: 1.2g/kg and 71 mg%; with all three lines displaying a robust first hour ADE. These findings suggest that (a) an ADE may be observed with short ethanol deprivation and re-exposure intervals in HAD rats, and (b) the genetic make-up of the P and HAD rats influences the expression of this ADE.
authors.affiliation: Department of Psychiatry, Institute of Psychiatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, USA. ribell@iupui.edu
authors.forename: Richard L
authors.initials: RL
authors.lastname: Bell
authors.forename: Zachary A
authors.initials: ZA
authors.lastname: Rodd
authors.forename: Jonathon A
authors.initials: JA
authors.lastname: Schultz
authors.forename: Caron L
authors.initials: CL
authors.lastname: Peper
authors.forename: Lawrence
authors.initials: L
authors.lastname: Lumeng
authors.forename: James M
authors.initials: JM
authors.lastname: Murphy
authors.forename: William J
authors.initials: WJ
authors.lastname: McBride
mesh_terms: D000428:Alcohol Drinking / Q000235:genetics / Q000523:psychology*; D000437:Alcoholism / Q000235:genetics / Q000523:psychology*; D000818:Animals; D016739:Behavior, Addictive / Q000235:genetics; D001522:Behavior, Animal / Q000187:drug effects*; D001835:Body Weight / Q000187:drug effects; D004326:Drinking / Q000187:drug effects; D004334:Drug Administration Schedule; D000431:Ethanol / Q000008:administration & dosage* / Q000097:blood; D005260:Female; D008297:Male; D051381:Rats; D011919:Rats, Inbred Strains; D012008:Recurrence; D012646:Self Administration
chemical_list: D000431:Ethanol