[Effect of neoadjuvant chemotherapy on ERCC1 gene expression in breast cancer].
OBJECTIVE
To investigate the expression of ERCC1 gene in breast cancer before and after neo-adjuvant chemotherapy.


METHODS
The expression of ERCC1 gene was detected by RT-PCR in 40 breast cancer patients and in 14 patients treated with neoadjuvant chemotherapy.


RESULTS
Positive expression of ERCC1 gene was detected by RT-PCR in 35.0% of the breast cancer specimens, and ERCC1 expression was not correlated to the patients' age, tumor size, axillary lymph node metastasis, pathological type, histological grade, ER, PR or HER-2 (P>0.05). ERCC1 gene expression was significantly higher in neo-adjuvant chemotherapy group than in non-chemotherapy group (P<0.05).


CONCLUSIONS
The expression of ERCC1 gene does not affect the clinical and pathological features of breast cancer. Neo-adjuvant chemotherapy can increase the expression of ERCC1 gene, due attention should be given to with in subsequent chemotherapy.
Fu
Jian-Min
JM
Department of Breast Diseases, Shenzhen Maternal and Child Health Hospital Affiliated to Southern Medical University, Shenzhen 518028, China. jmfu2000@yahoo.com.cn
Zhou
Jie
J
Xie
Jian-Sheng
JS
Li
Hui
H
D000328:Adult; D000368:Aged; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000627:therapeutic use*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000235:genetics*; D018270:Carcinoma, Ductal, Breast / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics*; D000077143:Docetaxel; D004720:Endonucleases / Q000235:genetics*; D005047:Etoposide / Q000008:administration & dosage; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008875:Middle Aged; D020360:Neoadjuvant Therapy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D043823:Taxoids / Q000008:administration & dosage
D004268:DNA-Binding Proteins; D043823:Taxoids; D000077143:Docetaxel; D005047:Etoposide; C071447:ERCC1 protein, human; D004720:Endonucleases