[Study on the interaction of tetra-sulfonated phathalocyanine cobalt with bovine serum albumin].
The tetra-sulfonated phathalocyanine cobalt(CoPcS4)was synthesized by using 4-sulfonic phthalic acid as raw material, CoCl2 as template agent and (NH4)2 MoO4 as catalyst. CoPcS4 was demonstrated to be C4h mono-isomer by elementary analysis, IR, NMR and HLPC. Depolymerization of CoPcS4 was found in DMSO. The depolymerization increased with the pH value raising. The interaction of tetra-sulfonated phathalocyanine cobalt with bovine serum albumin (BSA) was studied by UV-Vis and fluorescence spectra The number of binding sites and binding constant were determined at equal pace of the order of magnitude 10(5) L x mol(-1). CoPcS4 binds with BSA at Site I and Site II. In conclusion, COPcS4 binds with BSA commendably and albumin plays a role of storage and transport.
Huang
Yan
Y
Medical College of Shandong University, Ji'nan 250012, China.
Xu
Guo-Xing
GX
Peng
Yi-Ru
YR
Weng
Jia-Bao
JB
D000818:Animals; D002232:Carbocyanines / Q000737:chemistry*; D002417:Cattle; D003035:Cobalt / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013053:Spectrophotometry
D002232:Carbocyanines; D007211:Indoles; D054833:Isoindoles; D012710:Serum Albumin, Bovine; D003035:Cobalt; C013647:phthalocyanine