Antinociceptive and anti-inflammatory effects of the essential oil from Eremanthus erythropappus leaves.
The chemical composition of the essential oil from air-dried leaves of Eremanthus erythropappus was studied. The main compounds were beta-pinene (23.24%), beta-caryophyllene (22.92%), beta-myrcene (10.03%) and germacrene D (9.40%). The essential oil had an LD50 of 2.90 g kg(-1) in mice. Doses of 200 and 400 mg kg(-1) inhibited 10.69% and 27.06% of acetic-acid-induced writhing in mice, respectively. In the formalin-induced nociception test in mice, the essential oil inhibited the first phase of paw licking by 29.13% (400 mg kg(-1)) and the second phase by 32.74% (200 mg kg(-1)) and 37.55% (400 mg kg(-1)). In the hot-plate test in mice, doses of 200 mg kg(-1) and 400 mg kg(-1) significantly increased the reaction time after 30, 60 and 90 min of treatment. Doses of 200 and 400 mg kg(-1) inhibited carrageenan-induced paw oedema in rats by 15.18% and 36.61%, respectively. Doses of 200 and 400 mg kg(-1) administered 4 h before intrapleural injection of carrageenan significantly reduced exudate volume (by 20.20% and 48.70%, respectively) and leucocyte mobilization (by 5.88% and 17.29%, respectively). These results demonstrate that E. erythropappus has analgesic and anti-inflammatory properties, supporting the use of this plant in folk medicine.
Sousa
Orlando V
OV
Departamento Farmacêutico, Faculdade de Farmácia e Bioquímica, Universidade Federal de Juiz de Fora, 36016-330, Juiz de Fora, MG, Brazil. orlando.sousa@ufjf.edu.br
Silvério
Marcelo S
MS
Del-Vechio-Vieira
Glauciemar
G
Matheus
Filipe C
FC
Yamamoto
Célia H
CH
Alves
Maria S
MS
D000700:Analgesics / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D019659:Asteraceae / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000188:drug therapy; D007928:Lethal Dose 50; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D009822:Oils, Volatile / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D010146:Pain / Q000188:drug therapy; D010147:Pain Measurement; D010936:Plant Extracts / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D009822:Oils, Volatile; D010936:Plant Extracts