Cytotoxic activities of trichothecenes isolated from an endophytic fungus belonging to order Hypocreales.
Bioassay-guided fractionation of the extract of the endophytic fungus KLAR 5 belonging to order Hypocreales, which was isolated from the twig of Knema laurina (Blume) Warb., resulted in the isolation of brefeldin A (1), 8-deoxy-trichothecin (2), trichothecolone (3), 7alpha-hydroxytrichodermol (4), and 7alpha-hydroxyscirpene (5). Compound 5 was isolated from natural source for the first time. Compound 1 was very highly active against human epidermoid carcinoma of the mouth, human breast cancer (BC-1), and human small cell lung cancer (NCI-H187) cells whereas compounds 2 and 4 were selectively active against BC-1 and NCI-H187 cells. Compounds 3 and 5 were moderately active against these three cancer cell lines.
Chinworrungsee
Maneekarn
M
Department of Chemistry, Faculty of Science, Srinakharinwirot University, Bangkok, 10110, Thailand. maneekarn@swu.ac.th
Wiyakrutta
Suthep
S
Sriubolmas
Nongluksna
N
Chuailua
Phitaya
P
Suksamrarn
Apichart
A
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D006999:Hypocreales; D026324:Myristicaceae / Q000382:microbiology*; D014255:Trichothecenes / Q000302:isolation & purification / Q000494:pharmacology*
D000970:Antineoplastic Agents; D014255:Trichothecenes