[Function of F10 gene, a novel hydatidiform mole-related gene: a preliminary study].
OBJECTIVE
To study the function of F10 gene, a novel hydaditiform mole-related gene.


METHODS
A549 cell line was transfected with the F10 gene of forward or reverse sequence or with the empty vector, respectively. The cellular mRNA was extracted after 24 h of transfection to screen for the differentially expressed genes among the 3 transfected and the control cells using differential display-polymerase chain reaction (ddPCR).


RESULTS
The bands representing differentially expressed genes were amplified from the cells, and the products were linked to T-Vector for sequence analysis. Several genes were screened by Blasting and their expressions were confirmed by fluorescent quantitative PCR.


CONCLUSIONS
F10 gene is functionally related to cell proliferation and apoptosis.
Cao
Xiao-min
XM
Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Pang
Zhan-jun
ZJ
Quan
Song
S
Xing
Fu-qi
FQ
D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006828:Hydatidiform Mole / Q000235:genetics*; D002820:Hydatidiform Mole, Invasive / Q000235:genetics; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology; D009857:Oncogenes / Q000235:genetics*; D011247:Pregnancy; D014162:Transfection; D014594:Uterine Neoplasms / Q000235:genetics*