title: Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use.
abstract: Recent studies have shown that mesenchymal stem cells (MSC) with the potential for cell-mediated therapies and tissue engineering applications can be isolated from extracted dental tissues. Here, we investigated the collection, processing, and cryobiological characteristics of MSC from human teeth processed under current good tissue practices (cGTP). Viable dental pulp-derived MSC (DPSC) cultures were isolated from 31 of 40 teeth examined. Of eight DPSC cultures examined more thoroughly, all expressed appropriate cell surface markers and underwent osteogenic, adipogenic, and chondrogenic differentiation in appropriate differentiation medium, thus meeting criteria to be called MSC. Viable DPSC were obtained up to 120 h postextraction. Efficient recovery of DPSC from cryopreserved intact teeth and second-passage DPSC cultures was achieved. These studies indicate that DPSC isolation is feasible for at least 5 days after tooth extraction, and imply that processing immediately after extraction may not be required for successful banking of DPSC. Further, the recovery of viable DPSC after cryopreservation of intact teeth suggests that minimal processing may be needed for the banking of samples with no immediate plans for expansion and use. These initial studies will facilitate the development of future cGTP protocols for the clinical banking of MSC.
authors.affiliation: General BioTechnology, LLC, Indianapolis, Indiana, USA.
authors.forename: Brandon C
authors.initials: BC
authors.lastname: Perry
authors.forename: Dan
authors.initials: D
authors.lastname: Zhou
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Wu
authors.forename: Feng-Chun
authors.initials: FC
authors.lastname: Yang
authors.forename: Michael A
authors.initials: MA
authors.lastname: Byers
authors.forename: T-M Gabriel
authors.initials: TM
authors.lastname: Chu
authors.forename: J Jeffrey
authors.initials: JJ
authors.lastname: Hockema
authors.forename: Erik J
authors.initials: EJ
authors.lastname: Woods
authors.forename: W Scott
authors.initials: WS
authors.lastname: Goebel
mesh_terms: D017667:Adipocytes / Q000166:cytology; D000293:Adolescent; D000328:Adult; D002462:Cell Membrane / Q000378:metabolism; D064987:Cell- and Tissue-Based Therapy / Q000379:methods; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology; D015925:Cryopreservation / Q000379:methods*; D003782:Dental Pulp / Q000166:cytology*; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008963:Molar / Q000473:pathology; D010012:Osteogenesis; D023822:Tissue Engineering / Q000379:methods*