title: [Application of caffeine-halothane contracture test in the diagnosis of malignant hyperthermia].
abstract: OBJECTIVE
  To explore the application of caffeine-halothane contracture test (CHCT) in the confirmation of malignant hyperthermia (MH).
  
  
  METHODS
  One patient who underwent radical gastrectomy presented with clinical manifestations of MH during routine intravenous-inhalation anesthesia process. Isoflurane inhalation and the operation were ceased immediately and emergency management approaches such as physical cooling therapy were taken. Meanwhile, the levels of serum creatine kinase (CK), serum myoglobin, and urinary myoglobin were examined and rectus abdominis was taken and then CHCT was performed to confirm the clinical diagnosis. Total genome was extracted from the patient and then exons 2-18, 39-46, and 90-104 of ryanodine receptor 1 (RYR1) gene were screened to detect mutations using DNA sequencing technique.
  
  
  RESULTS
  The patient was diagnosed as MH episode by clinical characteristics and postoperatively continuous elevation of the levels of CK, serum myoglobin, and urinary myoglobin (30 times higher than normal level). Despite halothane test was negative, the diagnosis of MH was verified by the positive result of caffeine test. DNA sequencing of RYR1 gene of the patient revealed c. 6724C > T (p. T 2 206M).
  
  
  CONCLUSIONS
  CHCT can be used to confirm the diagnosis of MH.
authors.affiliation: Department of Anesthesiology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
authors.forename: Ying-Lin
authors.initials: YL
authors.lastname: Wang
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Guo
authors.forename: Zhong-Huang
authors.initials: ZH
authors.lastname: Xu
authors.forename: Yu-Guang
authors.initials: YG
authors.lastname: Huang
authors.forename: Ai-Lun
authors.initials: AL
authors.lastname: Luo
mesh_terms: D018685:Anesthetics, Inhalation / Q000627:therapeutic use; D002110:Caffeine; D003402:Creatine Kinase / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay; D006221:Halothane; D006801:Humans; D007530:Isoflurane / Q000627:therapeutic use; D008305:Malignant Hyperthermia / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D018482:Muscle, Skeletal / Q000187:drug effects / Q000503:physiopathology; D009211:Myoglobin / Q000097:blood; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics
chemical_list: D018685:Anesthetics, Inhalation; D009211:Myoglobin; D019837:Ryanodine Receptor Calcium Release Channel; D002110:Caffeine; D007530:Isoflurane; D003402:Creatine Kinase; D006221:Halothane