Ultrasmall c(RGDyK)-coated Fe3O4 nanoparticles and their specific targeting to integrin alpha(v)beta3-rich tumor cells.
We report a direct synthesis of ultrasmall c(RGDyK) peptide-coated Fe3O4 NPs (<10 nm in hydrodynamic diameter) and demonstrate their in vivo tumor-specific targeting capability. The Fe3O4 NPs are synthesized by thermal decomposition of iron pentacarbonyl in the presence of 4-methylcatechol (4-MC), and the peptide is coupled to the nanoparticles through 4-MC via Mannich reaction. The c(RGDyK)-MC-Fe3O4 NPs have an overall diameter of approximately 8.4 nm and are stable in physiological conditions. When administrated intravenously, these c(RGDyK)-MC-Fe3O4 NPs accumulate preferentially in the integrin alphavbeta3-rich tumor area, which are readily tracked by MRI.
Xie
Jin
J
Department of Chemistry, Brown University, Providence, Rhode Island 02912, USA.
Chen
Kai
K
Lee
Ha-Young
HY
Xu
Chenjie
C
Hsu
Andrew R
AR
Peng
Sheng
S
Chen
Xiaoyuan
X
Sun
Shouheng
S
D000818:Animals; D002396:Catechols / Q000737:chemistry; D045744:Cell Line, Tumor; D005290:Ferric Compounds / Q000737:chemistry*; D006801:Humans; D039302:Integrin alphaVbeta3 / Q000378:metabolism; D058085:Iron Compounds / Q000737:chemistry; D008279:Magnetic Resonance Imaging; D051379:Mice; D008815:Mice, Inbred Strains; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009369:Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D010456:Peptides, Cyclic / Q000737:chemistry*
D002396:Catechols; D005290:Ferric Compounds; D039302:Integrin alphaVbeta3; D058085:Iron Compounds; D010456:Peptides, Cyclic; C018599:4-methylcatechol; C000499:ferric oxide; C515100:iron pentacarbonyl