Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates.
An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC(50)12 microM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 microM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.
Kielar
Filip
F
Department of Chemistry, Durham University, South Road, Durham, UKDH1 3LE.
Congreve
Aileen
A
Law
Ga-lai
GL
New
Elizabeth J
EJ
Parker
David
D
Wong
Ka-Leung
KL
Castreno
Pilar
P
de Mendoza
Javier
J
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001120:Arginine / Q000737:chemistry*; D002451:Cell Compartmentation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005347:Fibroblasts / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D019791:Guanidine / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods; D008928:Mitochondria / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D008968:Molecular Conformation; D041681:NIH 3T3 Cells; D009942:Organometallic Compounds / Q000737:chemistry* / Q000493:pharmacokinetics; D010455:Peptides / Q000737:chemistry; D017785:Photons; D012709:Serum Albumin / Q000737:chemistry; D012867:Skin / Q000737:chemistry / Q000166:cytology; D013237:Stereoisomerism; D013725:Terbium / Q000737:chemistry*
D008024:Ligands; D009942:Organometallic Compounds; D010455:Peptides; D012709:Serum Albumin; D013725:Terbium; D001120:Arginine; D019791:Guanidine