Chronic constriction injury of the infraorbital nerve in the rat using modified syringe needle.
Here we report a method for performing a chronic constriction injury (CCI) of the infraorbital nerve (ION) in the rat as a component of a chronic pain model. The surgical approach to the ION is described together with the use of a modified dental syringe needle that simplifies placing two chromic gut ligatures around the ION. This method makes the surgical procedure easier, the nerve injury more consistent across animals and reduces secondary damage to the ION and surrounding tissue. Pain behavior testing together with immunostaining for markers of nerve injury in the spinal trigeminal nucleus show the suitability of this procedure as a model of orofacial pain.
Kernisant
Melanie
M
Department of Anatomy, University of California San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143, United States.
Gear
Robert W
RW
Jasmin
Luc
L
Vit
Jean-Philippe
JP
Ohara
Peter T
PT
D051700:Activating Transcription Factor 3 / Q000378:metabolism; D000818:Animals; D039481:CD11b Antigen / Q000378:metabolism; D003389:Cranial Nerve Diseases / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D005145:Face / Q000294:innervation; D008026:Ligation / Q000295:instrumentation* / Q000379:methods*; D008297:Male; D008442:Maxillary Nerve / Q000503:physiopathology; D009339:Needles; D051381:Rats; D017207:Rats, Sprague-Dawley; D018709:Statistics, Nonparametric; D013594:Syringes; D013997:Time Factors
D051700:Activating Transcription Factor 3; C497108:Atf3 protein, rat; D039481:CD11b Antigen