title: Regulation of electrolyte transport across cultured endometrial epithelial cells by prolactin.
abstract: The effect of prolactin (PRL) on ion transport across the porcine glandular endometrial epithelial cells was studied in primary cell culture using the short-circuit current technique. Addition of 1 microg/ml PRL either to the apical solution or to the basolateral solution produced a peak followed by a sustained increase in Isc, but with a lesser response when PRL was added apically. Basolateral addition of PRL increased the Isc in a concentration-dependent manner with a maximum effect at 1 microg/ml and an effective concentration value of 120 ng/ml. The PRL-stimulated Isc was significantly reduced by pretreatment with an apical addition of 5-nitro-2-(3-phenylpropylamino) benzoic acid (200 microM), diphenylamine-2-carboxylic acid (1 mM) or 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (200 microM), Cl(-) channel blockers, but not by amiloride (10 microM), a Na(+) channel blocker. In addition, pretreatment with bumetanide (200 microM), a Na(+)-K(+)-2Cl(-) cotransporter inhibitor, in the basolateral solution significantly reduced the PRL-stimulated Isc. Replacement of Cl(-) or in the bathing solutions also decreased the Isc response to PRL. Pretreatment of the monolayer with AG490 (50 microM), an inhibitor of JAK2 activity significantly inhibited the PRL-induced increase in Isc. Western blot analysis of the porcine endometrial epithelial cells revealed the presence of short isoform of PRL receptor (PRLR-S) that could be regulated by 17beta-estradiol. The results of this investigation showed that PRL acutely stimulated anion secretion across the porcine endometrial epithelial cells possibly through PRLR-S present in both apical and basolateral membranes. The PRL response appeared to be mediated by the JAK2-dependent pathway.
authors.affiliation: Department of Physiology, Faculty of Medicine, Srinakharinwirot University, Sukhumvit 23, Wattana, Bangkok 10110, Thailand. chatsri@swu.ac.th
authors.forename: Chatsri
authors.initials: C
authors.lastname: Deachapunya
authors.forename: Sutthasinee
authors.initials: S
authors.lastname: Poonyachoti
authors.forename: Nateetip
authors.initials: N
authors.lastname: Krishnamra
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D001639:Bicarbonates / Q000378:metabolism; D002712:Chlorides / Q000378:metabolism; D004573:Electrolytes / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D017136:Ion Transport / Q000187:drug effects; D053614:Janus Kinase 2 / Q000502:physiology; D009579:Nitrobenzoates / Q000494:pharmacology; D011188:Potassium / Q000378:metabolism; D011388:Prolactin / Q000494:pharmacology*; D011981:Receptors, Prolactin / Q000032:analysis; D015398:Signal Transduction; D012964:Sodium / Q000378:metabolism; D013552:Swine
chemical_list: D001639:Bicarbonates; D002712:Chlorides; D004573:Electrolytes; D009579:Nitrobenzoates; D011981:Receptors, Prolactin; C058176:5-nitro-2-(3-phenylpropylamino)benzoic acid; D011388:Prolactin; D012964:Sodium; D053614:Janus Kinase 2; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; D011188:Potassium