Isolation and imprinting analysis of the porcine DLX5 gene and its association with carcass traits.
Imprinted genes play important roles in mammalian growth and development. However, reports on imprinted genes are limited in livestock. In this study, the complete ORF containing 289 amino acids of the porcine DLX5 gene was obtained. A C-to-T SNP mutation in exon 1 of the DLX5 gene was used to detect imprinting status with an RT-PCR/RFLP test (using HhaI) in eight heterozygous pigs from a population of Large White x Meishan F(1) hybrids. Imprinting analysis showed that the porcine DLX5 gene was maternally expressed in skeletal muscle, fat, lung, spleen, stomach and small intestine, but not imprinted in heart, liver, kidney, uterus, ovary, testicle or pituitary. A PCR-RFLP test was also used to detect the polymorphism in 310 pigs of a Large White x Meishan F(2) resource population. The statistical results showed significant association (P < 0.01) of the genotypes and fat meat percentage, carcass length, bone percentage, 6-7 rib fat thickness, average backfat thickness, thorax-waist fat thickness and buttock fat thickness.
Cheng
H C
HC
Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China.
Zhang
F W
FW
Jiang
C D
CD
Li
F E
FE
Xiong
Y Z
YZ
Deng
C Y
CY
D000818:Animals; D003433:Crosses, Genetic; D015870:Gene Expression; D018392:Genomic Imprinting; D005838:Genotype; D018398:Homeodomain Proteins / Q000235:genetics*; D008460:Meat; D016366:Open Reading Frames; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D019655:Quantitative Trait, Heritable; D020133:Reverse Transcriptase Polymerase Chain Reaction; D034421:Sus scrofa / Q000235:genetics*; D014157:Transcription Factors / Q000235:genetics*
C088334:Distal-less homeobox proteins; D018398:Homeodomain Proteins; D014157:Transcription Factors