title: CXCL10 and IL-6 induce chemotaxis in human trophoblast cell lines.
abstract: The investigation of trophoblast chemoattractive molecules in humans is of high interest for the reproductive field. Current evidence in ruminants demonstrates that CXCL10, formerly the interferon-gamma-inducible protein 10 (IP-10), is a potent chemotactic molecule implicated in the migration of trophoblast cells during early gestation. The aim of this work was to explore the existence of CXCL10/CXCR3 in the human model. Furthermore, chemotaxis assays were performed to demonstrate CXCL10 chemotactic activity in the human trophoblast cell lines JEG-3 and AC-1M88. Surprisingly, the conditioned media from epithelial endometrial cells (EEC) induced the highest trophoblast migration rate. Cytokine and chemokine membrane protein arrays were used to identify the secreted protein profile of EEC-conditioned media, and IL-6 was found to be the most abundant and CXCL13 the second most abundant molecule. Using a chemotaxis assay on AC-IM88, IL-6 antibody blocked the effect of EEC, indicating IL-6 to be an effective chemoattractive factor for trophoblast cells in the human model.
authors.affiliation: Fundación Instituto Valenciano de Infertilidad (FIVI), University of Valencia, C/ Guadassuar 1, Bajo, 46015 Valencia, Spain.
authors.forename: F
authors.initials: F
authors.lastname: Dominguez
authors.forename: S
authors.initials: S
authors.lastname: Martínez
authors.forename: A
authors.initials: A
authors.lastname: Quiñonero
authors.forename: F
authors.initials: F
authors.lastname: Loro
authors.forename: J A
authors.initials: JA
authors.lastname: Horcajadas
authors.forename: A
authors.initials: A
authors.lastname: Pellicer
authors.forename: C
authors.initials: C
authors.lastname: Simón
mesh_terms: D000328:Adult; D001755:Blastocyst / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D054357:Chemokine CXCL10 / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D002633:Chemotaxis / Q000187:drug effects*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000494:pharmacology*; D008597:Menstrual Cycle / Q000378:metabolism; D040081:Protein Array Analysis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054367:Receptors, CXCR3 / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: C515187:CXCR3 protein, human; D054357:Chemokine CXCL10; D017077:Culture Media, Conditioned; D015850:Interleukin-6; D012333:RNA, Messenger; D054367:Receptors, CXCR3