Decoration of superparamagnetic iron oxide nanoparticles with Ni2+: agent to bind and separate histidine-tagged proteins.
The decoration of iron oxide nanoparticles with Ni2+ ions provided the superparamagnetic nanoparticles with a binding site for His-tagged proteins, allowing their selective binding and convenient separation from a multi-component solution with an appropriately applied magnetic field.
Lee
Kyung Sig
KS
Department of Chemistry & Advanced Material Sciences, College of Environment and Applied Chemistry, Kyung Hee University, Gyeonggido 449-701, Korea.
Lee
In Su
IS
D005290:Ferric Compounds / Q000737:chemistry*; D005453:Fluorescence; D006639:Histidine / Q000737:chemistry*; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
D005290:Ferric Compounds; D011506:Proteins; C000499:ferric oxide; D006639:Histidine; D009532:Nickel