Water-soluble derivative of propolis mitigates scopolamine-induced learning and memory impairment in mice.
The water-soluble derivative of propolis (WSDP) was prepared from fresh Chinese propolis. Its major constituents were identified by high performance liquid chromatography (HPLC) analysis. It has been reported that propolis possessed a broad spectrum of biological activities but including few studies on learning and memory by now. Thus, this study was aimed to investigate the effect of WSDP on scopolamine-induced learning and memory impairment in mice. WSDP (50 mg/kg, 100 mg/kg) was given by intragastric administration (i.g.) 40 min prior to the intraperitoneal (i.p.) injection of scopolamine (1 mg/kg). The effect on amnesia was investigated with both hidden-platform acquisition training and probe trial testing in Morris water maze test. The results from 100 mg/kg WSDP group showed significant mitigation scopolamine-induced amnesia in mice. Furthermore, WSDP's effect on the acetylcholinesterase (AChE) activity in the cerebral cortex and hippocampus was also assayed. As a result, WSDP (100 mg/kg) significantly inhibited AChE activity in the hippocampus of scopolamine-treated mice. These results indicated that WSDP may mitigate amnesia in vivo through inhibition of AChE activity in the hippocampus, which suggested propolis may have potential as a pharmaceutical of brain protection with elderly population for preventing Alzheimer's disease (AD) and other neurodegenerative diseases.
Chen
Juan
J
Institute of Biochemistry and Molecular Biology, Lanzhou University, 222 Tian Shui South Road, Lanzhou, 730000, PR China.
Long
Yuan
Y
Han
Min
M
Wang
Ting
T
Chen
Qiang
Q
Wang
Rui
R
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D001120:Arginine / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology; D002851:Chromatography, High Pressure Liquid; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D007859:Learning Disabilities / Q000139:chemically induced / Q000517:prevention & control* / Q000523:psychology*; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008569:Memory Disorders / Q000139:chemically induced / Q000517:prevention & control* / Q000523:psychology*; D051379:Mice; D018727:Muscarinic Antagonists / Q000633:toxicity*; D018697:Nootropic Agents / Q000494:pharmacology; D010889:Piracetam / Q000494:pharmacology; D011429:Propolis / Q000737:chemistry / Q000494:pharmacology*; D012601:Scopolamine / Q000037:antagonists & inhibitors* / Q000633:toxicity*
D018727:Muscarinic Antagonists; D018697:Nootropic Agents; D011429:Propolis; D001120:Arginine; D012601:Scopolamine; D000110:Acetylcholinesterase; D010889:Piracetam