Evaluation of quantitative anti-F1 IgG and anti-V IgG ELISAs for use as an in vitro-based potency assay of plague vaccine in mice.
Quantitative anti-F1 and anti-V IgG enzyme-linked immunosorbent assays (ELISAs) were developed to measure the serological response of female Swiss Webster mice after vaccination with the recombinant fusion protein, rF1-V, which is being developed as a plague vaccine. Several fundamental parameters of the ELISA were evaluated: specificity, precision, accuracy, and stability. Experimental results suggested that a potency assay based upon the serological response of female Swiss Webster mice, as measured by quantitative anti-F1 IgG and anti-V IgG ELISAs, might be used to evaluate the rF1-V fusion protein vaccine.
Little
S F
SF
United States Army Medical Research Institute of Infectious Disease, Bacteriology Division, 1425 Porter Street, Fort Detrick, MD 21702-5011, USA. stephen.little@amedd.army.mil
Webster
W M
WM
Wilhelm
H
H
Powell
B
B
Enama
J
J
Adamovicz
J J
JJ
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D051379:Mice; D010931:Plague Vaccine / Q000276:immunology* / Q000592:standards*; D012015:Reference Standards; D015203:Reproducibility of Results; D013379:Substrate Specificity
D007074:Immunoglobulin G; D010931:Plague Vaccine