title: H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens.
abstract: Using plasmid-based reverse genetics, we generated a molecularly altered virus, H5N1/PR8-5B19, containing modified HA and NA genes from A/Goose/Guangdong/1/96 (GS/GD/1/96). In the H5N1/PR8-5B19 virus, the HA cleavage site was modified to resemble that of low-pathogenic avian strains and a portion of the NA stalk region was replaced by the immunodominant 5B19 epitope of the S2 glycoprotein of murine hepatitis virus (MHV). H5N1/PR8-5B19 is not lethal to embryonated eggs or chickens. Chickens immunized with the H5N1/PR8-5B19 inactivated vaccine produced high levels of HI antibody and a measurable antibody response against the MHV 5B19 epitope, and were fully protected against subsequent challenge with different highly pathogenic H5N1 avian influenza viruses. H5N1/PR8-5B19 is therefore an attractive marker vaccine candidate, eliciting a strong, protective antibody response and enabling serological discrimination between vaccinated and wild-type virus-infected chickens.
authors.affiliation: Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin, Heilongjiang 150001, People's Republic of China.
authors.forename: Chengjun
authors.initials: C
authors.lastname: Li
authors.forename: Jihui
authors.initials: J
authors.lastname: Ping
authors.forename: Bo
authors.initials: B
authors.lastname: Jing
authors.forename: Guohua
authors.initials: G
authors.lastname: Deng
authors.forename: Yongping
authors.initials: Y
authors.lastname: Jiang
authors.forename: Yanbing
authors.initials: Y
authors.lastname: Li
authors.forename: Guobin
authors.initials: G
authors.lastname: Tian
authors.forename: Kangzhen
authors.initials: K
authors.lastname: Yu
authors.forename: Zhigao
authors.initials: Z
authors.lastname: Bu
authors.forename: Hualan
authors.initials: H
authors.lastname: Chen
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000096:biosynthesis / Q000097:blood; D000917:Antibody Formation; D002645:Chickens / Q000276:immunology* / Q000821:virology*; D006023:Glycoproteins / Q000235:genetics / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D016056:Immunodominant Epitopes / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology* / Q000627:therapeutic use; D005585:Influenza in Birds / Q000517:prevention & control*; D006517:Murine hepatitis virus / Q000235:genetics / Q000276:immunology; D009439:Neuraminidase / Q000235:genetics / Q000276:immunology; D014611:Vaccination; D019444:Vaccines, DNA; D015164:Vaccines, Inactivated / Q000235:genetics / Q000276:immunology / Q000627:therapeutic use; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D006023:Glycoproteins; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D016056:Immunodominant Epitopes; D007252:Influenza Vaccines; D019444:Vaccines, DNA; D015164:Vaccines, Inactivated; D014764:Viral Proteins; C487630:NA protein, influenza A virus; D009439:Neuraminidase