Spatial and temporal down-regulation of transgene expression using the TRSID-silencer in mice: application to Prnp.
Spatial and temporal control of ovine prion protein (Prnp) gene expression was achieved in mice using two transgenes: a Prnp minigene with tet-operator sequences inserted 5' to exon 1 and a mouse neurofilament genomic clone carrying the chimeric-repressor TRSID cDNA. In bi-transgenic mice, ovine PrP(C) expression could be reversibly controlled in neuronal cells by doxycycline treatment whereas it remains constant in other cell types. Overall, this model opens opportunities to assess the involvement of cell types in prion diseases and PrP physiological function. It demonstrates the potentiality of the TRSID-silencer to precisely control temporal and spatial gene expression in vivo.
Gallozzi
Micaela
M
Laboratoire de Génétique Biochimique et de Cytogénétique, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France.
Béringue
Vincent
V
Decaunes
Pauline
P
Le Dur
Annick
A
Le Roux
Karine
K
Tilly
Gaëlle
G
Le Guillou
Sandrine
S
Herzog
Laetitia
L
Peyre
Coralie
C
Ladroue
Aline
A
Chapuis
Jérôme
J
Vilotte
Marthe
M
Passet
Bruno
B
Costa
José
J
Chenais
Nathalie
N
Le Provost
Fabienne
F
Laude
Hubert
H
Vilotte
Jean-Luc
JL
D000818:Animals; D015536:Down-Regulation; D015870:Gene Expression; D051379:Mice; D008822:Mice, Transgenic; D009474:Neurons / Q000378:metabolism; D018621:PrPC Proteins / Q000235:genetics*; D012756:Sheep / Q000235:genetics*; D037941:Silencer Elements, Transcriptional; D019076:Transgenes
D018621:PrPC Proteins