title: TNF-induced IL-8 and MCP-1 production in the eosinophilic cell line, EOL-1.
abstract: The role of eosinophils in inflammation and their mode of activation is not well understood. Eosinophil accumulation and subsequent expression of cytokines at the site of inflammation may play a role in exacerbation of inflammatory responses. In the present study, we have examined the role of TNF-alpha in eosinophil activation and chemokine production using a human leukaemic eosinophil cell line, EOL-1. Initial studies demonstrated that TNF-alpha induced the upregulation of IL-8 and MCP-1 mRNA and protein. Kinetic studies indicated production of chemokines, IL-8 and MCP-1, as early as 4 h post-activation, with peak levels of chemokine produced at 8 h, and decreasing by 24 h post-TNF-alpha activation. When IL-10, a suppressive cytokine, was incubated with TNF-alpha and EOL-1 cells, no effect was observed on IL-8 and MCP-1 production. However, dexamethasone, a glucocorticoid, demonstrated potent inhibitory effects on the EOL-1-derived chemokines. These studies indicate that eosinophils may be a significant source of chemokines capable of participating in, and maintaining, leukocyte recruitment during inflammatory responses, such as asthma.
authors.affiliation: Department of Pathology Division of Pulmonary and Critical Care University of Michigan Medical School 1301 Catherine Ann Arbor MI 48109-0602 USA.
authors.forename: L A
authors.initials: LA
authors.lastname: Goldstein
authors.forename: R M
authors.initials: RM
authors.lastname: Strieter
authors.forename: H L
authors.initials: HL
authors.lastname: Evanoff
authors.forename: S L
authors.initials: SL
authors.lastname: Kunkel
authors.forename: N W
authors.initials: NW
authors.lastname: Lukacs