title: Evaluation of a multiplex flow cytometric immunoassay to detect PR3- and MPO-ANCA in active and treated vasculitis, and in inflammatory bowel disease (IBD).
abstract: This study compared the performance of a flow cytometric immunoassay for antineutrophil cytoplasmic antibodies (ANCA) directed against proteinase 3 (PR3) and myeloperoxidase (MPO), with indirect immunofluorescence (IIF) and ELISAs from 12 different manufacturers. Sera were from patients with active (n=55) or treated (n=68) small vessel vasculitis, or inflammatory bowel disease (IBD, n=22). The immunoassay specificity was 88% compared with 96% for IIF and 94% (median, range 91-96%) for both ELISAs. Its sensitivity in treated disease was 82% compared with 84% for IIF and 69% (median, range 57-82%) for the ELISAs. The immunoassay's specificity was 88% which was the same as the median for both ELISAs (range 84-95%). The PR3- and MPO-ANCA immunoassay was almost as sensitive as IIF, and more sensitive than, but just as specific as, most ELISAs, in detecting ANCA in active and treated vasculitis. A major advantage of this assay is its ability to be further modified to simultaneously screen for a panel of autoantibodies relevant to vasculitis.
authors.affiliation: Pathology Department, Austin Health, Heidelberg, VIC 3084, Australia.
authors.forename: Michelle
authors.initials: M
authors.lastname: Trevisin
authors.forename: Wendy
authors.initials: W
authors.lastname: Pollock
authors.forename: Wayne
authors.initials: W
authors.lastname: Dimech
authors.forename: Judy
authors.initials: J
authors.lastname: Savige
mesh_terms: D019268:Antibodies, Antineutrophil Cytoplasmic / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005434:Flow Cytometry / Q000379:methods*; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D015212:Inflammatory Bowel Diseases / Q000097:blood / Q000175:diagnosis*; D053146:Myeloblastin / Q000097:blood*; D009195:Peroxidase / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014657:Vasculitis / Q000097:blood / Q000175:diagnosis*
chemical_list: D019268:Antibodies, Antineutrophil Cytoplasmic; D009195:Peroxidase; D053146:Myeloblastin