Effects of epidermal growth factor on the invasion activity of the oral cancer cell lines HSC3 and SAS.
Epidermal growth factor (EGF) is excreted in a high concentration in human saliva and modulates the growth and differentiation of various cancer cells. To elucidate the molecular mechanisms by which EGF affects oral cancer growth and invasion, we analyzed the Matrigel invasion activity of the cultured oral cancer cell line. Cells grown under the influence of EGF were subjected to Matrigel invasion assays and cells grown in the absence of EGF were used as controls. Gelatin-zymography and Northern blot analyses quantified the invasiveness and tumorigenicity. Chloramphenicol acetyltransferase assay (CAT assay) determined the EGF stimulation of matrix metalloproteinase (MMP) expression. EGF increased the number of cells penetrating a Matrigel membrane. Gelatin-zymography and Northern blot analysis revealed that MMP9 and Ets1 expressions correlated with EGF but MMP2 was not changed. a transient transfection assay revealed that EGF increased the promoter activities of the MMP9 genes in HSC3 and SAS cells. These results suggest that EGF increases the invasion activity of oral cancer cells partly by increasing MMP9.
Ohnishi
Yuichi
Y
Second Department of Oral and Maxillofacial Surgery, Osaka Dental University, 1-5-17 Otemae, Chuo-ku, 540-0008 Osaka, Japan. onishi_yu_ku@msn.com
Lieger
Olivier
O
Attygalla
Manjula
M
Iizuka
Tateyuki
T
Kakudo
Kenji
K
D001672:Biocompatible Materials; D015152:Blotting, Northern; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D015500:Chloramphenicol O-Acetyltransferase / Q000378:metabolism; D003094:Collagen; D004338:Drug Combinations; D004815:Epidermal Growth Factor / Q000494:pharmacology*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007797:Laminin; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D009062:Mouth Neoplasms / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness; D011509:Proteoglycans; D012463:Saliva / Q000378:metabolism; D014407:Tumor Cells, Cultured
D001672:Biocompatible Materials; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D004815:Epidermal Growth Factor; D003094:Collagen; D015500:Chloramphenicol O-Acetyltransferase; D020780:Matrix Metalloproteinase 9