title: Retinal arteriolar and middle cerebral artery responses to combined hypercarbic/hyperoxic stimuli.
abstract: OBJECTIVE
  The relative effect of simultaneously administered oxygen and carbon dioxide on the retinal and cerebral vessels is still controversial. The purpose of this study was to quantify and compare the superior-temporal retinal arteriole (RA) and middle cerebral artery (MCA) responses to hypercarbic and combined hypercarbic/hyperoxic stimuli.
  
  
  METHODS
  Twelve young, healthy volunteers participated in the study. End-tidal pressure of carbon dioxide was raised and maintained at 22% from baseline (hypercarbia), while end-tidal pressures of oxygen (P(ET)O(2)) of 100 (normoxia), 500, and 300 mm Hg (hyperoxia) were instituted. RA diameter and blood velocity were measured with laser Doppler velocimetry and simultaneous vessel densitometry; MCA blood velocity was measured with transcranial Doppler ultrasound.
  
  
  RESULTS
  Normoxic hypercarbia increased RA blood velocity by +17% and calculated flow by +21%. Hypercarbia/hyperoxia-500 mm Hg decreased RA diameter by -8%, velocity by -16% and calculated flow by -29%. MCA blood velocity increased by +45% in response to normoxic hypercarbia, significantly greater than RA blood velocity (P < 0.001). Increase in P(ET)O(2) did not affect the hypercarbia-induced increase in MCA blood velocity.
  
  
  CONCLUSIONS
  Hyperoxia reversed hypercarbia-induced vasodilation in RA in a concentration-dependent manner. Hypercarbia induced greater vasodilation in the MCA than in the RA but MCA blood velocity was unaffected by increases in P(ET)O(2).
authors.affiliation: Department of Ophthalmology and Vision Science, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Mila
authors.initials: M
authors.lastname: Kisilevsky
authors.forename: Alexandra
authors.initials: A
authors.lastname: Mardimae
authors.forename: Marat
authors.initials: M
authors.lastname: Slessarev
authors.forename: Jay
authors.initials: J
authors.lastname: Han
authors.forename: Joseph
authors.initials: J
authors.lastname: Fisher
authors.forename: Chris
authors.initials: C
authors.lastname: Hudson
mesh_terms: D000328:Adult; D001160:Arterioles / Q000502:physiology; D001783:Blood Flow Velocity / Q000502:physiology; D001794:Blood Pressure; D002245:Carbon Dioxide / Q000008:administration & dosage; D002536:Cerebral Arteries / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology; D005260:Female; D006801:Humans; D006935:Hypercapnia / Q000503:physiopathology*; D018496:Hyperoxia / Q000503:physiopathology*; D017078:Laser-Doppler Flowmetry; D008297:Male; D010100:Oxygen / Q000008:administration & dosage; D010313:Partial Pressure; D012039:Regional Blood Flow / Q000502:physiology; D012161:Retinal Artery / Q000502:physiology*; D017585:Ultrasonography, Doppler, Transcranial; D014664:Vasodilation
chemical_list: D002245:Carbon Dioxide; D010100:Oxygen