title: [Correlation of the autophagosome gene ATG16L1 polymorphism and inflammatory bowel disease].
abstract: OBJECTIVE
  To understand the relationship between the susceptibility to inflammatory bowel disease (IBD) and ATG16L1 gene single nucleotide polymorphism (SNP) site, rs2241880.
  
  
  METHODS
  Peripheral blood samples were collected from 80 IBD patients (including 40 with Crohn's disease and 40 with ulcerative colitis) and 50 healthy controls, and the genomic DNA was extracted from the white blood cells. Specific primers were designed according to the target gene sequence for PCR amplification of the target gene fragment, and the PCR products were purified followed by sequence analysis of the target region of ATG16L1 gene. The results of the sequence analysis were compared with the BenBank data to analyze the relationship between the allele gene polymorphisms and the susceptibility to Crohn's disease.
  
  
  RESULTS
  No significant differences were noted in the ATG16L1 gene SNP site rs2241880 polymorphisms among the patients with Crohn's disease, ulcerative colitis and the control subjects (Chi(2)=4.94, P=0.293).
  
  
  CONCLUSIONS
  ATG16L1 gene polymorphisms in the SNP site rs2241880 are not found to correlate to the susceptibility to Crohn's disease as reported in literature. The SNP site associated with Crohn's disease susceptibility identified in foreign populations does not seem to be identical with that in Chinese population.
authors.affiliation: Institute of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. pancula@163.com
authors.forename: Jia
authors.initials: J
authors.lastname: Zhi
authors.forename: Fa-chao
authors.initials: FC
authors.lastname: Zhi
authors.forename: Zheng-yan
authors.initials: ZY
authors.lastname: Chen
authors.forename: Guo-peng
authors.initials: GP
authors.lastname: Yao
authors.forename: Jing
authors.initials: J
authors.lastname: Guan
authors.forename: Yong
authors.initials: Y
authors.lastname: Lin
authors.forename: Ying-chun
authors.initials: YC
authors.lastname: Zhang
mesh_terms: D000328:Adult; D001343:Autophagy / Q000235:genetics; D000071183:Autophagy-Related Proteins; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003093:Colitis, Ulcerative / Q000235:genetics; D003424:Crohn Disease / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics; D006801:Humans; D015212:Inflammatory Bowel Diseases / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010588:Phagosomes / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
chemical_list: C518959:ATG16L1 protein, human; D000071183:Autophagy-Related Proteins; D002352:Carrier Proteins