The K/BxN arthritis model.
Mice expressing both the T cell receptor (TCR) transgene KRN and the MHC class II molecule A(g7) (K/BxN mice) develop severe inflammatory arthritis, and serum from these mice causes a similar arthritis in a wide range of mouse strains, due to autoantibodies recognizing glucose-6-phosphate isomerase (GPI). K/BxN transgenic mice have been useful for investigating the development of autoimmunity, and the serum transfer model has been particularly valuable in eliciting mechanisms by which anti-GPI autoantibodies induce joint-specific inflammation. This unit describes detailed methods for the maintenance of a K/BxN colony, induction of arthritis by serum transfer, clinical evaluation of arthritis, and measurement of anti-GPI antibodies.
Monach
Paul A
PA
Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts.
Mathis
Diane
D
Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts.
Benoist
Christophe
C
Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts.
D000818:Animals; D001168:Arthritis / Q000201:enzymology / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D001323:Autoantibodies / Q000097:blood; D016913:Blood Component Transfusion; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005956:Glucose-6-Phosphate Isomerase / Q000276:immunology; D000949:Histocompatibility Antigens Class II / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D016688:Mice, Inbred NOD; D008822:Mice, Transgenic; D016133:Polymerase Chain Reaction; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D001323:Autoantibodies; D000949:Histocompatibility Antigens Class II; C407099:I-A g7 antigen; D011948:Receptors, Antigen, T-Cell; D005956:Glucose-6-Phosphate Isomerase