[Expression of eosinophil major basic protein and neutrophil elastase in nasal polyp tissue and secretion].
OBJECTIVE
To explore the role in the pathogenesis of nasal polyp (NP) by comparison of eosinophil major basic protein (MBP), and neutrophil elastase (NE) in nasal polyps (NP) epithelium, stroma and the secretion of expression.


METHODS
Immunohistochemical detection of 30 cases of patients with chronic sinusitis (CRS) NP epithelium NE stroma and the expression and secretion of MBP.


RESULTS
1. There were significant differences of the expression of NE and MBP in epithelial tissue, stroma and secretion compared with the control group (P < 0.05); 2. There was not significant difference of the expression of NE and MBP between epithelial tissue and stroma (P > 0.05), while there was significant difference between epithelial tissue and the secretion (P < 0.05); 3. There were significant differences of the average positive expression of MBP and NE among epithelial tissue, stroma and secretion (P < 0.05); 4. MBP and NE were usually degranulated in secretion, while usually located in eosinophils (Eos) and neutrophils (Neu) in epithelial and mesenchymal; 5. There were abundant expression of MBP and NE in epithelial shedding regional, while small amounts of expression in stroma and integrated epithelial; 6. Electron microscopy could show the characteristics of electron density of MBP and NE particles.


CONCLUSIONS
MBP and NE collaborated to cause pathological effects on the occurrence of NP.
Zhang
Chun
C
Department of Otolaryngology-Head and Neck Surgery, Renji Hospital of Jiaotong University, Medical College, Shanghai, 200001, China. zcjbsx@163.com
Jiang
Yiming
Y
Chen
Jie
J
Zheng
Luying
L
Wang
Jiadong
J
Shen
Huifeng
H
D000293:Adolescent; D000328:Adult; D000368:Aged; D001798:Blood Proteins / Q000378:metabolism*; D012634:Bodily Secretions / Q000378:metabolism; D047093:Eosinophil Major Basic Protein; D004804:Eosinophils / Q000378:metabolism; D005260:Female; D006801:Humans; D007958:Leukocyte Count; D019272:Leukocyte Elastase / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000378:metabolism* / Q000473:pathology; D011509:Proteoglycans / Q000378:metabolism*; D012852:Sinusitis / Q000378:metabolism* / Q000473:pathology; D055815:Young Adult
D001798:Blood Proteins; D011509:Proteoglycans; C074129:PRG2 protein, human; D047093:Eosinophil Major Basic Protein; D019272:Leukocyte Elastase