Molecular evidence for apoptosis in microfilariae of Wuchereria bancrofti induced by diethylcarbamazine.
Apoptosis, a programmed cell death, is characterized by chromatin condensation, numerous vacuoles, reduction in cell volume, and endonuclease cleavage DNA degradation detected in gel electrophoresis as nucleosomal ladder. Here we report that diethylcarbamazine induces DNA fragmentation in microfilariae of Wuchereria bancrofti revealed by ligation-mediated polymerase chain reaction and by in situ terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling at the light and electron transmission level.
Peixoto
C A
CA
Laboratório de Ultraestrutura, Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães (FIOCRUZ), Recife, Brazil. cpeixoto@cpqam.fiocruz.br
Santos
A C O
AC
Ayres
C F J
CF
D000818:Animals; D017209:Apoptosis; D053938:DNA Fragmentation; D018509:DNA, Helminth / Q000378:metabolism; D004049:Diethylcarbamazine / Q000494:pharmacology*; D005369:Filaricides / Q000494:pharmacology*; D020287:In Situ Nick-End Labeling; D008842:Microfilariae / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure; D008853:Microscopy; D046529:Microscopy, Electron, Transmission; D016133:Polymerase Chain Reaction; D014958:Wuchereria bancrofti / Q000166:cytology / Q000187:drug effects* / Q000648:ultrastructure
D018509:DNA, Helminth; D005369:Filaricides; D004049:Diethylcarbamazine