Cytotoxicity of hydroxyapatite, fluorapatite and fluor-hydroxyapatite: a comparative in vitro study.
The purpose of this study was to evaluate the cytotoxicity of two formulations of hydroxyapatite (HA), namely fluorapatite (FA) and fluor-hydroxyapatite (FHA). HA is used as carrier material for antibiotics or anticancer drugs during treatment of bone metastasis. Negative control, represented by HA, was included for comparative purposes. Leukemia cells were used as a model cell line, and the effect of eluates of tested biomaterials on cell proliferation/viability and mechanism of antiproliferative activity were assessed. Study design attempted to reveal the toxicity of tested biomaterials with an emphasis to decide if tested biomaterials have promise for further studies in vivo. Results showed that eluates of FA and FHA inhibit the growth of leukemia cells and induce programmed cell death through mitochondrial/caspase-9/caspase-3-dependent pathway. Due to these differences compare to HA, it is concluded that FA and FHA have promise for evaluation of their behaviour in vivo.
Theiszova
M
M
Institute of Biochemistry, Nutrition and Health Protection, Faculty of Chemical and Food Technology, Slovak University of Technology, Bratislava, Slovakia.
Jantova
S
S
Letasiova
S
S
Palou
M
M
Cipak
L
L
D000818:Animals; D001031:Apatites / Q000494:pharmacology*; D017209:Apoptosis; D001672:Biocompatible Materials / Q000494:pharmacology; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D004337:Drug Carriers / Q000494:pharmacology*; D004353:Drug Evaluation, Preclinical; D017886:Durapatite / Q000494:pharmacology*; D006882:Hydroxyapatites / Q000494:pharmacology*; D007939:Leukemia L1210; D008422:Materials Testing; D051379:Mice
D001031:Apatites; D001672:Biocompatible Materials; D004337:Drug Carriers; D006882:Hydroxyapatites; C028869:fluor-hydroxylapatite; D017886:Durapatite; D053148:Caspase 3; D053453:Caspase 9; C025105:fluorapatite