Cytomegalovirus in colorectal cancer and idiopathic ulcerative colitis.
Cytomegalovirus (CMV) is a genus in the family Herpesviridae that has been associated with gastrointestinal syndromes. In this work we looked for a possible association of CMV infection with colorectal cancer and ulcerative colitis (UC). Blood and enteric tissue samples of 14 patients with colorectal cancer and of 21 with UC were subjected to a nested-PCR that amplifies part of the gB gene of CMV and also to immunohistochemistry using a specific monoclonal antibody to IE 76 kDa protein of CMV. CMV was detected by nested-PCR in the blood and/or the enteric tissue of nine (64.3%) colorectal cancer and 16 (76.2%) ulcerative colitis patients. In the immunohistochemistry it was observed that 12 (12/21, 57.1%) positive enteric tissue samples of patients with UC and none from patients with colorectal cancer (0/14) were positive to CMV. The positivity of CMV infections in the UC patient group (12/21, 57.1%) showed by both techniques, was significantly higher (p = 0.015) than that observed for colorectal cancer patients (2/14, 14.3%). These results suggest an association of ulcerative colitis with CMV infection of the enteric tissue.
Mariguela
Viviane Casagrande
VC
Virology Research Center, School of Medicine, University of São Paulo, Ribeirão Preto City, SP, Brazil.
Chacha
Silvana Gama Florêncio
SG
Cunha
Aldo de Albuquerque
Ade A
Troncon
Luiz Ernesto de Almeida
LE
Zucoloto
Sérgio
S
Figueiredo
Luiz Tadeu Moraes
LT
D003093:Colitis, Ulcerative / Q000821:virology*; D015179:Colorectal Neoplasms / Q000821:virology*; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000150:complications* / Q000175:diagnosis; D004279:DNA, Viral / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry; D016133:Polymerase Chain Reaction
D004279:DNA, Viral