Comparison of natural and recombinant forms of the major fish allergen parvalbumin from cod and carp.
Allergic reaction following fish consumption can trigger life-threatening reactions in predisposed individuals. Parvalbumins from different species have been identified as the major fish allergens. There are two distinct phylogenetic lineages of parvalbumins, alpha and beta. Most allergic reactions are caused by beta-parvalbumins. We cloned and expressed cDNAs encoding cod (Gadus morhua) and carp (Cyprinus carpio) beta-parvalbumins and purified natural cod beta-parvalbumin. CD spectra of the purified proteins showed that their overall secondary structure contents were very similar. No differences in thermal stability were monitored in the calcium-bound or calcium-depleted form of natural cod parvalbumin. IgE reactivity was assessed using 26 sera of fish allergic patients from Spain, The Netherlands, and Greece in immunoblot and ELISA experiments. Twenty-five of the 26 patients with IgE reactivity to native and recombinant cod parvalbumin also reacted to the recombinant carp parvalbumin. IgE inhibition assays were performed using cod and carp extracts and purified recombinant parvalbumin of cod and carp. High crossreactivity among cod and carp parvalbumins was observed in immunoblots as well as in fluid phase assays. Natural and recombinant parvalbumins gave comparable results when performing various in vitro diagnostic assays.
Ma
Yan
Y
Center for Physiology, Pathophysiology and Immunology, Department of Pathophysiology, Medical University of Vienna, Vienna, Austria.
Griesmeier
Ulrike
U
Susani
Markus
M
Radauer
Christian
C
Briza
Peter
P
Erler
Anja
A
Bublin
Merima
M
Alessandri
Stefano
S
Himly
Martin
M
Vàzquez-Cortés
Sonia
S
de Arellano
Isabel Reig Rincon
IR
Vassilopoulou
Emilia
E
Saxoni-Papageorgiou
Photini
P
Knulst
André C
AC
Fernández-Rivas
Montserrat
M
Hoffmann-Sommergruber
Karin
K
Breiteneder
Heimo
H
D000485:Allergens / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D002347:Carps / Q000276:immunology*; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003429:Cross Reactions; D048388:Gadiformes / Q000276:immunology*; D006863:Hydrogen-Ion Concentration; D007073:Immunoglobulin E / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D008969:Molecular Sequence Data; D010320:Parvalbumins / Q000737:chemistry* / Q000276:immunology / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification
D000485:Allergens; D010320:Parvalbumins; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D002118:Calcium