In vivo anti-inflammatory and antinociceptive actions of some Lamium species.
BACKGROUND
Several Lamium species have been used to relieve pain in arthritic ailments in Turkish folk medicine.


OBJECTIVE
To evaluate the anti-inflammatory and antinociceptive activities of different extracts prepared with methanol, dichloromethane, n-butanol and water from the aerial parts of some Lamium species of Turkish origin.


METHODS
Lamium eriocephalum subsp. eriocephalum, Lamium garganicum subsp. laevigatum, Lamium garganicum subsp. pulchrum and Lamium purpureum var. purpureum (Lamiaceae); for the anti-inflammatory activity, carrageenan-induced hind paw edema model, PGE(2)-induced hind paw edema model, and 12-O-tetradecanoyl-13-acetate (TPA)-induced mouse ear edema model and for the antinociceptive activity p-benzoquinone (PBQ)-induced writhing test in mice were employed.


RESULTS
The n-butanol extracts of Lamium garganicum subsp. laevigatum (LGL-BuOH), Lamium garganicum subsp. pulchrum (LGP-BuOH), and Lamium purpureum var. purpureum (LPP-BuOH) exhibited notable inhibition (16.5-28.9, 14.5-26.9, 12.3-21.5%, resp.) in carrageenan-induced hind paw edema model at doses of 200mg/kg without inducing any gastric damage. The LGL-BuOH (7.1-30.4%) and LGP-BuOH (5.9-24.1%) extracts also displayed potent anti-inflammatory activity against PGE(2)-induced hind paw edema model. LGL-BuOH and LGP-BuOH were also found to exhibit remarkable antinociceptive activity in p-benzoquinone-induced abdominal constriction test at a dose of 200 mg/kg (25.0 and 24.3%, respectively).


CONCLUSIONS
The experimental data demonstrated that Lamium garganicum subsp. laevigatum and Lamium garganicum subsp. pulchrum displayed remarkable anti-inflammatory and antinociceptive activities.
Akkol
Esra Küpeli
EK
Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Etiler, Ankara, Turkey. esrak@gazi.edu.tr
Yalçin
Funda Nuray
FN
Kaya
Duygu
D
Caliş
Ihsan
I
Yesilada
Erdem
E
Ersöz
Tayfun
T
D000700:Analgesics / Q000008:administration & dosage* / Q000009:adverse effects / Q000302:isolation & purification; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage* / Q000009:adverse effects / Q000302:isolation & purification; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007249:Inflammation / Q000188:drug therapy; D019686:Lamiaceae / Q000737:chemistry*; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D010146:Pain / Q000188:drug therapy; D010936:Plant Extracts / Q000008:administration & dosage* / Q000009:adverse effects; D014421:Turkey
D000700:Analgesics; D000893:Anti-Inflammatory Agents; D010936:Plant Extracts