title: Determination of melamine in pet food by enzyme immunoassay, high-performance liquid chromatography with diode array detection, and ultra-performance liquid chromatography with tandem mass spectrometry.
abstract: Melamine in pet food (fortified or originally contaminated) was determined by enzyme immunoassay (EIA), high-performance liquid chromatography with diode array detection (HPLC-DAD), and ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS). The limits of detection (LOD) for EIA and HPLC-DAD were 0.02 and 0.1 microg/mL, respectively. The linear ranges of the calibration curves for EIA and HPLC-DAD were 0.02-0.5 and 0.1-500 microg/mL, respectively. The coefficient of determinations (r2) of the standard curves for EIA and HPLC were 0.9991 and 0.9999, respectively. Coefficient of variations from both inter- and intra-assay were <9.31%, and recovery range for all concentrations was between 71 and 105%. The r2 values between the EIA and HPLC-DAD methods for melamine analysis of the fortified and originally contaminated samples were 0.9973 and 0.9885. The r2 values for UPLC-MS/MS with HPLC-DAD and with EIA were 0.9566 and 0.9489, respectively.
authors.affiliation: University of Maine, Department of Food Science and Human Nutrition, 5735 Hitchner Hall, Orono, ME 04469-5735, USA.
authors.forename: Byungchul
authors.initials: B
authors.lastname: Kim
authors.forename: Lewis B
authors.initials: LB
authors.lastname: Perkins
authors.forename: Rodney J
authors.initials: RJ
authors.lastname: Bushway
authors.forename: Stephanie
authors.initials: S
authors.lastname: Nesbit
authors.forename: Titan
authors.initials: T
authors.lastname: Fan
authors.forename: Robert
authors.initials: R
authors.lastname: Sheridan
authors.forename: Virginia
authors.initials: V
authors.lastname: Greene
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D007124:Immunoenzyme Techniques / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014227:Triazines / Q000032:analysis*
chemical_list: D014227:Triazines; C011907:melamine