Nanofluidic biosensing for beta-amyloid detection using surface enhanced Raman spectroscopy.
Trace detection of the conformational transition of beta-amyloid peptide (Abeta) from a predominantly alpha-helical structure to beta-sheet could have a large impact in understanding and diagnosing Alzheimer's disease. We demonstrate how a novel nanofluidic biosensor using a controlled, reproducible surface enhanced Raman spectroscopy active site was developed to observe Abeta in different conformational states during the Abeta self-assembly process as well as to distinguish Abeta from confounder proteins commonly found in cerebral spinal fluid.
Chou
I-Hsien
IH
Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843-3120, USA.
Benford
Melodie
M
Beier
Hope T
HT
Coté
Gerard L
GL
Wang
Miao
M
Jing
Nan
N
Kameoka
Jun
J
Good
Theresa A
TA
D016229:Amyloid beta-Peptides / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
D016229:Amyloid beta-Peptides