Epithelial V-like antigen regulates permeability of the blood-CSF barrier.
Epithelial V-like antigen (EVA), a CD3-binding immunoglobulin-like protein, regulates embryonic thymic development. Here we demonstrate that EVA is expressed in choroid plexus from mature immune competent and lymphocyte-deficient (RAG-/-) mice. Choroid plexus epithelial cells from RAG-/- mice demonstrated reduced junctional integrity and enhanced permeability that was associated with decreased expression of E-cadherin and EVA mRNA as compared to wild-type mice. Following iv infusion of an anti-CD3 antibody (145-2C11) that also binds EVA, expression of E-cadherin and EVA mRNA approached levels seen in wild-type mice. Immuno-fluorescent staining for cadherin also revealed decreased expression in untreated RAG-/- mice that could be increased by 145-2C11 treatment. Expression of mouse EVA in HEK-293 cells followed by challenge with 145-2C11 resulted in increased cytosolic calcium that was not seen in control cells. These results suggest that EVA expressed in choroid plexus cells may regulate the permeability of the blood-CSF barrier.
Chatterjee
Gouri
G
Department of Neurology, Yale University School of Medicine, PO Box 208018, New Haven, CT 06520-8018, USA.
Carrithers
Lisette M
LM
Carrithers
Michael D
MD
D000818:Animals; D001812:Blood-Brain Barrier / Q000276:immunology*; D015820:Cadherins / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D002460:Cell Line; D002831:Choroid Plexus / Q000276:immunology; D003600:Cytosol / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D008214:Lymphocytes / Q000276:immunology; D051379:Mice; D008817:Mice, Mutant Strains; D010539:Permeability
D015820:Cadherins; D015815:Cell Adhesion Molecules; C504470:Eva1 protein, mouse; D018398:Homeodomain Proteins; C064658:RAG-1 protein; D002118:Calcium