title: Comparison of positive tuberculin skin test with an interferon-gamma-based assay in unexposed children.
abstract: A false-positive tuberculin skin test (TST) may be a result of T-cell sensitivity due to Bacille Calmette-Guerin (BCG) vaccination or exposure to non-tuberculous mycobacteria, thus leading to unnecessary isoniazid preventive therapy, especially in low-risk populations. Unlike TST, T-SPOT.TB is not confounded by BCG vaccination or exposure to most of the other non-tuberculous mycobacteria, because this assay is based on enumeration of interferon-gamma-secreting T cells in response to Mycobacterium tuberculosis-specific antigens. We compared the TST with T-SPOT.TB with respect to different TST cut-off points in healthy unexposed BCG-vaccinated schoolchildren. A total of 209 children between 6 and 10 years of age with a TST induration of 0 (n = 50), 10 - 14 (n = 45), 15 - 19 (n = 95) and > or =20 mm (n = 19) were enrolled. Among TST-positive subjects, only 26 (23%) were positive with T-SPOT.TB, and T-SPOT.TB was positive in 4, 7, 20 and 42% of children with TST indurations of 0, 10 - 14, 15 - 19 and > or =20 mm, respectively. We suggest that confirmation of a positive TST by the interferon-gamma-based test would reduce unnecessary preventive therapy significantly in healthy unexposed BCG-vaccinated children.
authors.affiliation: Department of Pediatrics, Marmara University School of Medicine, Istanbul, Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Soysal
authors.forename: Ozden
authors.initials: O
authors.lastname: Türel
authors.forename: Demet
authors.initials: D
authors.lastname: Toprak
authors.forename: Mustafa
authors.initials: M
authors.lastname: Bakir
mesh_terms: D001500:BCG Vaccine / Q000008:administration & dosage; D002648:Child; D005189:False Positive Reactions; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D014374:Tuberculin Test; D014376:Tuberculosis / Q000175:diagnosis* / Q000453:epidemiology / Q000517:prevention & control*; D014421:Turkey / Q000453:epidemiology
chemical_list: D001500:BCG Vaccine; D007371:Interferon-gamma