Optimization of allograft implantation using scaffold-free chondrocyte plates.
If a tissue-engineered cartilage transplant is to succeed, it needs to integrate with the host tissue, to endure physiological loading, and to acquire the phenotype of the articular cartilage. Although there are many reported treatments for osteochondral defects of articular cartilage, problems remain with the use of artificial matrices (scaffolds) and the stage of implantation. We constructed scaffold-free three-dimensional tissue-engineered cartilage allografts using a rotational culture system and investigated the optimal stage of implantation and repair of the remodeling site. We evaluated the amounts of extracellular matrix and gene expression levels in scaffold-free constructs and transplanted the constructs for osteochondral defects using a rabbit model. Allografted 2-week constructs expressed high levels of proteoglycan and collagen per DNA content, integrated with the host cartilage successfully, and were able to counter physiological loads, and the chondrocyte plate contributed reparative mesenchymal stem cells to the final phenotype of the articular cartilage.
Nagai
Toshihiro
T
Department of Orthopedic Surgery and Surgical Science, School of Medicine, Tokai University, Kanagawa, Japan.
Sato
Masato
M
Furukawa
Katsuko S
KS
Kutsuna
Toshiharu
T
Ohta
Naoshi
N
Ushida
Takashi
T
Mochida
Joji
J
D000818:Animals; D001705:Bioprosthesis; D002358:Cartilage, Articular / Q000293:injuries / Q000378:metabolism / Q000473:pathology; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism / Q000473:pathology; D005260:Female; D017102:Fracture Healing; D050723:Fractures, Bone / Q000378:metabolism / Q000473:pathology / Q000628:therapy*; D005786:Gene Expression Regulation; D059630:Mesenchymal Stem Cells / Q000378:metabolism / Q000473:pathology; D010329:Patella / Q000293:injuries / Q000473:pathology; D011509:Proteoglycans / Q000096:biosynthesis; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods; D014184:Transplantation, Homologous; D016474:Weight-Bearing
D011509:Proteoglycans