title: Potential application of alkaline pectinase from Bacillus subtilis SS in pulp and paper industry.
abstract: Pectinase production from Bacillus subtilis SS was optimized under solid-state fermentation (5,943 U/g of dry bacterial bran). The pectinase produced was stable in neutral to alkaline pH range at 70 degrees C; therefore, the suitability of this pectinase in pulp and paper industry was investigated. The enzyme pretreatment process was optimized, and a pectinase dose of 5 IU/g of oven-dried pulp (10% consistency) at pH 9.5 temperature 70 degrees C after 150 min of treatment gave the best pretreatment to the pulp. An increase of 4.3% in brightness along with an increase of 14.8 and 65.3% in whiteness and fluorescence, respectively, whereas a 15% decrease in the yellowness of the pretreated pulp were observed. There was a 5.85% reduction in kappa number and 6.1% reduction in permanganate number along with a reduction in the chemical oxygen demand value. Significant characteristics showed by pectinase open new possibilities of application of this cellulase-free enzyme in the pulp and paper industry by reducing the negative environmental impact of chemicals apart from improving the properties of paper.
authors.affiliation: Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India.
authors.forename: Sonia
authors.initials: S
authors.lastname: Ahlawat
authors.forename: R P
authors.initials: RP
authors.lastname: Mandhan
authors.forename: Saurabh Sudha
authors.initials: SS
authors.lastname: Dhiman
authors.forename: Rakesh
authors.initials: R
authors.lastname: Kumar
authors.forename: Jitender
authors.initials: J
authors.lastname: Sharma
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology*; D010209:Paper; D011096:Polygalacturonase / Q000378:metabolism*; D013783:Textile Industry
chemical_list: D011096:Polygalacturonase