Protecting, patterning, and scaffolding supported lipid membranes using carbohydrate glasses.
Disaccharides are known to protect sensitive biomolecules against stresses caused by dehydration, both in vivo and in vitro. Here we demonstrate how interfacial accumulation of trehalose can be used to (1) produce rugged supported lipid bilayers capable of near total dehydration; (2) enable spatial patterning of membrane micro-arrays; and (3) form stable bilayers on otherwise lipophobic substrates (e.g., metal transducers) thus affording protecting, patterning, and scaffolding of lipid bilayers.
Oliver
Ann E
AE
Department of Applied Science, University of California-Davis, 1 Shields Ave., Davis, CA 95616, USA. aeoliver@ucdavis.edu
Kendall
Eric L
EL
Howland
Michael C
MC
Sanii
Babak
B
Shreve
Andrew P
AP
Parikh
Atul N
AN
D002241:Carbohydrates / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008670:Metals / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014159:Transducers; D014199:Trehalose / Q000737:chemistry; D014867:Water / Q000737:chemistry
D002241:Carbohydrates; D008051:Lipid Bilayers; D008670:Metals; D010743:Phospholipids; D014867:Water; D014199:Trehalose