Genotoxicity studies on licorice flavonoid oil (LFO).
Licorice flavonoid oil (LFO) is a new functional food ingredient. In this study, the genotoxicity of LFO was investigated using a test battery of three different methods. In a reverse mutation assay using four Salmonella typhimurium strains and Escherichia coli, LFO did not increase the number of revertant colonies in any tester strain with or without metabolic activation by rat liver S9 mix. In a chromosomal aberration test using Chinese hamster lung (CHL/IU) cells, LFO did not induce any chromosomal aberrations either in the short period test without rat liver S9 mix or in the continuous treatment (24 h or 48 h) test. However, in the short-period test with rat liver S9 mix, LFO induced structural chromosomal aberrations at concentrations higher than 0.6 mg/mL. A bone marrow micronucleus test using male F344 rats was initially conducted. The animals were dosed by oral gavage at doses up to 5000 mg/kg/day. No significant or dose-dependent increases in the frequency of micronucleated polychromatic erythrocytes (MNPCE) were observed and the high dose suppressed the ratio of polychromatic erythrocytes (PCE) to total erythrocytes. Subsequently, a liver and peripheral blood micronucleus test using male F344 rats was conducted. No micronuclei induction either in hepatocytes or PCE was observed even at the highest dose of 5000 mg/kg/day. From the findings obtained from the genotoxicity assays performed in this study and the published pharmacokinetic studies of LFO, it appears unlikely that dietary consumption of LFO will present any genotoxic hazard to humans.
Nakagawa
K
K
Functional Food Ingredients Division, Healthcare Products Business Unit, Kaneka Corporation, 3-2-4, Nakanoshima, Kita-ku, Osaka 530-8288, Japan.
Hidaka
T
T
Kitano
M
M
Asakura
M
M
Kamigaito
T
T
Noguchi
T
T
Hosoe
K
K
D000284:Administration, Oral; D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000139:chemically induced*; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004912:Erythrocytes / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005419:Flavonoids / Q000633:toxicity*; D006035:Glycyrrhiza / Q000737:chemistry*; D006801:Humans; D015162:Micronucleus Tests; D009152:Mutagenicity Tests; D009153:Mutagens; D010938:Plant Oils / Q000633:toxicity; D051381:Rats; D011916:Rats, Inbred F344; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics
D005419:Flavonoids; D009153:Mutagens; D010938:Plant Oils