Characterizing protein-protein interactions by sedimentation velocity analytical ultracentrifugation.
This unit introduces the basic principles and practice of sedimentation velocity analytical ultracentrifugation for the study of reversible protein interactions, such as the characterization of self-association, heterogeneous association, multi-protein complexes, binding stoichiometry, and the determination of association constants. The analytical tools described include sedimentation coefficient and molar mass distributions, multi-signal sedimentation coefficient distributions, Gilbert-Jenkins theory, different forms of isotherms, and global Lamm equation modeling. Concepts for the experimental design are discussed, and a detailed step-by-step protocol guiding the reader through the experiment and the data analysis is available as an Internet resource.
Brown
Patrick H
PH
National Institutes of Health, Bethesda, Maryland.
Balbo
Andrea
A
National Institutes of Health, Bethesda, Maryland.
Schuck
Peter
P
National Institutes of Health, Bethesda, Maryland.
D000818:Animals; D001665:Binding Sites; D002021:Buffers; D006801:Humans; D007700:Kinetics; D008956:Models, Chemical; D046912:Multiprotein Complexes / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D025941:Protein Interaction Mapping; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D014461:Ultracentrifugation / Q000379:methods*
D002021:Buffers; D046912:Multiprotein Complexes; D011506:Proteins