The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in Escherichia coli.
We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localization.
Donald
James W
JW
Department of Molecular Microbiology, John Innes Centre, Colney Lane, Norwich NR4 7UH, United Kingdom.
Hicks
Matthew G
MG
Richardson
David J
DJ
Palmer
Tracy
T
D001425:Bacterial Outer Membrane Proteins / Q000032:analysis*; D001426:Bacterial Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D003580:Cytochromes / Q000032:analysis* / Q000235:genetics*; D004926:Escherichia coli / Q000235:genetics; D021381:Protein Transport; D011994:Recombinant Proteins / Q000032:analysis / Q000235:genetics; D020592:Shewanella / Q000235:genetics*
D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D003580:Cytochromes; D011994:Recombinant Proteins