title: A reference integrated map for cultivated grapevine (Vitis vinifera L.) from three crosses, based on 283 SSR and 501 SNP-based markers.
abstract: We have developed an integrated map from five elite cultivars of Vitis vinifera L.; Syrah, Pinot Noir, Grenache, Cabernet Sauvignon and Riesling which are parents of three segregating populations. A new source of markers, SNPs, identified in ESTs and unique BAC-end sequences was added to the available IGGP reference set of SSRs. The complete integrated map comprises 1,134 markers (350 AFLP, 332 BESs, 169 ESTs, 283 SSRs) spanning 1,443 cM over 19 linkage groups and shows a mean distance between neighbouring loci of 1.27 cM. Marker order was mainly conserved between the integrated map and the highly dense SyrahxPinot Noir consensus map except for few inversions. Moreover, the marker order has been validated through the assembled genome sequence of Pinot Noir. We have also assessed the transferability of SNP-based markers among five V. vinifera varieties, enabling marker validation across different genotypes. This integrated map can serve as a fundamental tool for molecular breeding in V. vinifera and related species and provide a basis for studies of genome organization and evolution in grapevines.
authors.affiliation: IASMA Research Centre, San Michele a/Adige, Italy. silvia.vezzulli@iasma.it
authors.forename: Silvia
authors.initials: S
authors.lastname: Vezzulli
authors.forename: Michela
authors.initials: M
authors.lastname: Troggio
authors.forename: Giuseppina
authors.initials: G
authors.lastname: Coppola
authors.forename: Angelica
authors.initials: A
authors.lastname: Jermakow
authors.forename: Dustin
authors.initials: D
authors.lastname: Cartwright
authors.forename: Andrey
authors.initials: A
authors.lastname: Zharkikh
authors.forename: Marco
authors.initials: M
authors.lastname: Stefanini
authors.forename: M Stella
authors.initials: MS
authors.lastname: Grando
authors.forename: Roberto
authors.initials: R
authors.lastname: Viola
authors.forename: Anne-Françoise
authors.initials: AF
authors.lastname: Adam-Blondon
authors.forename: Mark
authors.initials: M
authors.lastname: Thomas
authors.forename: Patrice
authors.initials: P
authors.lastname: This
authors.forename: Riccardo
authors.initials: R
authors.lastname: Velasco
mesh_terms: D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers; D018745:Genome, Plant; D006824:Hybridization, Genetic; D018598:Minisatellite Repeats; D020641:Polymorphism, Single Nucleotide; D027843:Vitis / Q000235:genetics*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers