[Changes of protein kinase C activity in experimental presyrinx state in rabbits].
OBJECTIVE
To investigate the changes of protein kinase C (PKC) activity and its role in the development of presyrinx state in rabbits.


METHODS
Presyrinx state was established in 56 rabbits by intra-cisternal injection of kaolin. At 1, 3, 7, 14, and 21 days after the injection, the water content in the upper cervical spinal cord was measured, its pathological changes observed microscopically and the PKC activity determined with substrate phosphorolysis kinase assay.


RESULTS
Spinal cord edema occurred in rabbits one day after kaolin injection, with water content of (68.35-/+0.70)%, which increased to (72.70-/+0.88)% on day 3, reaching the peak level of (72.92-/+0.86)% on day 7, followed by gradual decline after 3 weeks [(70.03-/+0.77)%]. The membrane PKC activity increased from 5.67-/+0.26 pmol.mg(-1).min(-1) on day 1 after the injection to reach the peak level on day 7 (13.27-/+3.15 pmol.mg(-1).min(-1)), which was maintained till day 14 with subsequent decrease to 8.85-/+1.56 pmol.mg(-1).min(-1) on day 21. The cytoplasmic PKC activity showed changes of a reverse pattern.


CONCLUSIONS
In rabbits with experimental presyrinx state, PKC translocation and activation is involved in ischemic spinal edema.
Sun
Guo-zhu
GZ
Department of Neurosurgery, Second Hospital of Hebei Medical University, Shijiazhuang 050000, China. sungzh705@sohu.com
Hu
Qing-shan
QS
Zhang
Qing-jun
QJ
Zhao
Zong-mao
ZM
Zhang
Geng-shen
GS
D000818:Animals; D005260:Female; D007616:Kaolin; D008297:Male; D011493:Protein Kinase C / Q000378:metabolism*; D011817:Rabbits; D011897:Random Allocation; D013116:Spinal Cord / Q000201:enzymology*; D013595:Syringomyelia / Q000139:chemically induced / Q000201:enzymology*
D007616:Kaolin; D011493:Protein Kinase C