TEX101, a germ cell-marker glycoprotein, is associated with lymphocyte antigen 6 complex locus k within the mouse testis.
In adult male mice, the glycosylphosphatidyl inositol-anchored glycoprotein TEX101 is expressed only in germ cells and is thought to be involved in spermatogenesis. However, the details regarding the function of TEX101 remain to be clarified. We previously identified Ly6k as a candidate TEX101-associated protein, but as molecular probes are not currently available to detect Ly6k, we do not have conclusive evidence of the association between TEX101 and Ly6k. In this study, we confirmed the biological interaction between TEX101 and Ly6k using an established anti-mouse Ly6k polyclonal antibody (pAb). A combination of immunoprecipitation, Western blot, and immunohistochemical analyses using the pAb revealed that TEX101 is physically associated with Ly6k within the testis. In addition, these proteins simultaneously co-migrate into the detergent-resistant membrane fractions, suggesting that TEX101 collaborates with Ly6k on the cell membrane and may play a role in spermatogenesis.
Yoshitake
Hiroshi
H
Institute for Environmental & Gender-specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu City, Chiba, Japan.
Tsukamoto
Hiroki
H
Maruyama-Fukushima
Mayuko
M
Takamori
Kenji
K
Ogawa
Hideoki
H
Araki
Yoshihiko
Y
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal / Q000378:metabolism*; D000950:Antigens, Ly / Q000378:metabolism*; D000954:Antigens, Surface / Q000378:metabolism*; D015153:Blotting, Western; D002462:Cell Membrane / Q000378:metabolism; D002499:Centrifugation, Density Gradient; D005260:Female; D058851:GPI-Linked Proteins; D006023:Glycoproteins / Q000378:metabolism*; D047468:Immunoprecipitation; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D011817:Rabbits; D013091:Spermatogenesis; D013737:Testis / Q000378:metabolism*
D000906:Antibodies; D000911:Antibodies, Monoclonal; D000950:Antigens, Ly; D000954:Antigens, Surface; D058851:GPI-Linked Proteins; D006023:Glycoproteins; C526827:Ly6 protein, mouse; C426726:TEX101 protein, mouse