title: Correlations between the in vitro and in vivo bioactivity of the Ti/HA composites fabricated by a powder metallurgy method.
abstract: Ti/HA composites were successfully prepared by a powder metallurgy method and the effect of phase composition on the in vitro and in vivo bioactivity of the Ti/HA composites was investigated in the present study. The correlations between the in vitro and in vivo biological behaviors were highlighted. The results showed that the in vitro and in vivo bioactivity of the Ti/HA composites was dependent on their phase composition. The in vitro bioactivity of the Ti/HA composites was evaluated in simulated body fluid with ion concentrations similar to those of human plasma. After immersion in the simulated body fluid for a certain time, apatite precipitations formed on the surface of the composites with an initial titanium content of 50 and 70 wt.%, and no apatite was found on the surface of the composite with 30% titanium. Ti(2)O was responsible for the apatite formation on the surfaces of the composites. For in vivo analysis, Ti/HA cylinders were implanted in the metaphases of the rabbit femur. At the early stage of implantation, the new bone formed on the surface of the composite with 30% titanium was much less than that on the surfaces of the composites with 50% and 70% titanium. All the Ti/HA composites formed a chemical bone-bonding interface with the host bone by 6 months after implantation. The Ti/HA composites formed the bone-bonding interface with the surrounding bone through an apatite layer. The results in the present study suggested that the in vivo results agreed well with the in vitro results.
authors.affiliation: Biomaterials and Tissue Engineering Center, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China.
authors.forename: Congqin
authors.initials: C
authors.lastname: Ning
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D001826:Body Fluids / Q000737:chemistry; D018786:Bone Substitutes / Q000737:chemistry; D001842:Bone and Bones / Q000378:metabolism; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D066298:In Vitro Techniques; D007477:Ions; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D011208:Powders; D011817:Rabbits; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D007477:Ions; D011208:Powders; D017886:Durapatite; D014025:Titanium