[Simultaneous determination of aconitine analogues in Aconitum plants and foods that caused food poisoning by liquid chromatography with tandem mass spectrometry].
A simple method for the simultaneous determination of four aconitine analogues (AC; aconitine, HA; hypaconitine, MA; mesaconitine, JA; jesaconitine) in Aconitum plants (Aconitum subcuneatum NAKAI) and a food that caused food poisoning was developed, using liquid chromatography tandem mass spectrometry (LC/MS/MS). Aconitine analogues were extracted with 1 mmol/L HCl and then cleaned up with an Oasis HLB cartridge. The LC separation was performed with an octadecylated silica column (Develosil ODS-HG-5, 2.0 mm i.d. x 50 mm) at a flow rate of 0.2 mL/min, using A solution (5 mmol/L ammonium acetate dissolved in 0.1% acetic acid) and B solution (acetonitrile-THF=1 : 3), 90%A (0 min)-->60%A (15 min)-->const. (2 min). Mass spectral acquisition was performed in the positive mode and the analogues were targeted using multiple reaction monitoring (MRM) with electrospray ionization (ESI). The recoveries of aconitine analogues were 93-99% from Aconitum plants. The detection limits of AC, HA, MA and JA were 0.4, 0.4, 0.3 and 0.5 ng/g, respectively. The aconitine analogues, except JA, were detected in food that caused food poisoning at the level of 2.6-29.7 microg/g. These results indicate that the developed method is suitable for the determination of aconitine analogues in Aconitum plants and foods that cause food poisoning.
Kasahara
Yoshimasa
Y
The Yamagata Prefectural Institute of Public Health, Yamagata-Shi, Yamagata, Japan.
Itou
Takeshi
T
D000157:Aconitine / Q000009:adverse effects / Q000031:analogs & derivatives / Q000032:analysis* / Q000302:isolation & purification*; D031841:Aconitum / Q000737:chemistry*; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis; D005517:Foodborne Diseases / Q000209:etiology*; D053719:Tandem Mass Spectrometry / Q000379:methods*
C058309:hypaconitine; C019470:mesaconitine; C069418:jesaconitine; D000157:Aconitine