title: A new pancreatic lipase inhibitor isolated from the roots of Actinidia arguta.
abstract: A new coumaroyl triterpene, 3-O-trans-p-coumaroyl actinidic acid (1), as well as five known triterpenes, ursolic acid (2), 23-hydroxyursolic acid (3), corosolic acid (4), asiatic acid (5) and betulinic acid (6) were isolated from an EtOAc-soluble extract of the roots of Actinidia arguta. The structure of compound 1 was elucidated from interpretation of the spectroscopic data, particularly by extensive 1D and 2D NMR studies. All the isolates (1-6) were evaluated in vitro for their inhibitory activities on pancreatic lipase (PL). Of the isolates, the new compound 1 possessed the highest inhibitory activity on PL, with an IC(50) of 14.95 microM, followed by ursolic acid (2, IC(50) = 15.83 microM). The other four triterpenes (3-6) also showed significant PL inhibitory activity, with IC(50) values ranging from 20.42 to 76.45 microM.
authors.affiliation: Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, Daejeon, 305-811, Korea.
authors.forename: Dae Sik
authors.initials: DS
authors.lastname: Jang
authors.forename: Ga Young
authors.initials: GY
authors.lastname: Lee
authors.forename: Junghyun
authors.initials: J
authors.lastname: Kim
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Jong Min
authors.initials: JM
authors.lastname: Kim
authors.forename: Young Sook
authors.initials: YS
authors.lastname: Kim
authors.forename: Jin Sook
authors.initials: JS
authors.lastname: Kim
mesh_terms: D029042:Actinidia; D000818:Animals; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D010179:Pancreas / Q000201:enzymology*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots; D013552:Swine; D014315:Triterpenes / Q000302:isolation & purification* / Q000494:pharmacology
chemical_list: C530811:3-O-p-coumaroyl actinidic acid; D010936:Plant Extracts; D014315:Triterpenes; D008049:Lipase