Multidimensional fluorescence microscopy of multiple organelles in Arabidopsis seedlings.
BACKGROUND
The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. In planta analyses of this dynamic process have typically been limited to only two organelles or proteins at a time in only a few cell types.


RESULTS
We generated a transgenic Arabidopsis plant that contains four spectrally different fluorescent proteins. Nuclei, plastids, mitochondria and plasma membranes were genetically tagged with cyan, red, yellow and green fluorescent proteins, respectively. In addition, methods to track nuclei, mitochondria and chloroplasts and quantify the interaction between these organelles at a submicron resolution were developed. These analyzes revealed that N-ethylmaleimide disrupts nuclear-mitochondrial but not nuclear-plastids interactions in root epidermal cells of live Arabidopsis seedlings.


CONCLUSIONS
We developed a tool and associated methods for analyzing the complex dynamic of organelle-organelle interactions in real time in planta. Homozygous transgenic Arabidopsis (Kaleidocell) is available through Arabidopsis Biological Resource Center.
Kato
Naohiro
N
Louisiana State University, Department of Biological Sciences, Baton Rouge, LA, 70803, USA. kato@LSU.edu.
Reynolds
Dexter
D
Brown
Matthew L
ML
Boisdore
Marietta
M
Fujikawa
Yukichi
Y
Morales
Andrea
A
Meisel
Lee A
LA