title: Analysis of tetracycline residues in royal jelly by liquid chromatography-tandem mass spectrometry.
abstract: A confirmatory method coupling liquid chromatography with tandem mass spectrometry (LC/MS/MS) was developed to determine the concentration of oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC) and doxycycline (DC), which make up the tetracycline (TC) groups present in royal jelly. Sample preparation included deproteination, control of pH, extraction and clean-up on a solid-phase extraction (SPE) cartridge. The analyses were achieved by LC/MS/MS in selected reaction monitoring mode (SRM). The overall recovery of fortified royal jelly at the levels of 5.0, 10.0 and 40.0 microg/kg ranged from 62% to 115%, and the coefficients of variation ranged from 3.4% to 16.3% (n=6). The detection limits for TCs were under 1.0 microg/kg. The transformation between the TCs and its epimers (EpiTCs) was studied in standard solution and during the sample preparation process. This method can be used for the detection of tetracycline residues in royal jelly.
authors.affiliation: Animal, Plant and Food Inspection Center, Jiangsu Entry-Exit Inspection and Quarantine Bureau, National Bee-Product Reference Laboratory, Nanjing, PR China. xujz@jsciq.gov.cn
authors.forename: Jin-Zhong
authors.initials: JZ
authors.lastname: Xu
authors.forename: Tao
authors.initials: T
authors.lastname: Ding
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Wen-Quan
authors.initials: WQ
authors.lastname: Yang
authors.forename: Xiao-Yan
authors.initials: XY
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Chong-Yu
authors.initials: CY
authors.lastname: Shen
authors.forename: Yuan
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D004350:Drug Residues / Q000032:analysis*; D005227:Fatty Acids / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013752:Tetracycline / Q000032:analysis*; D000099219:Royal Jelly
chemical_list: D000900:Anti-Bacterial Agents; D005227:Fatty Acids; D013752:Tetracycline; D000099219:Royal Jelly