title: Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do, Korea.
abstract: Bovine tuberculosis (TB) is a major zoonosis that's caused by Mycobacterium bovis (M. bovis). Being able to detect M. bovis is important to control bovine TB. We applied a molecular technique, the variable number tandem repeat (VNTR) typing method, to identify and distinguish the M. bovis isolates from Gyeonggi-do, Korea. From 2003 to 2004, 59 M. bovis clinical strains were isolated from dairy cattle in Gyeonggi-do, Korea, and these cattle had tuberculosis- like lesions. Twenty-four published MIRUVNTR markers were applied to the M. bovis isolates and ten of them showed allelic diversity. The most discriminatory locus for the M. bovis isolates in Korea was QUB 3336 (h = 0.64). QUB 26 and MIRU 31 also showed high discriminative power (h = 0.35). The allelic diversity by the combination of all VNTR loci was 0.86. Six loci (MIRU 31, ETR-A and QUB-18, -26, -3232, -3336) displayed valuable allelic diversity. Twelve genotypes were identified from the 59 M. bovis isolates that originated from 20 cattle farms that were dispersed throughout the region of Gyenggi-do. Two genotypes [designation index (d.i.) = e, g] showed the highest prevalence (20% of the total farms). For the multiple outbreaks on three farms, two successive outbreaks were caused by the same genotype at two farms. Interestingly, the third outbreak at one farm was caused by both a new genotype and a previous genotype. In conclusion, this study suggests that MIRU-VNTR typing is useful to identify and distinguish the M. bovis isolates from Gyeonggi-do, Korea.
authors.affiliation: Department of Microbiology and the Brain Korea 21 Project for the Medical Sciences, Yonsei University College of Medicine, Seoul 120-752, Korea.
authors.forename: Bo- Young
authors.initials: B
authors.lastname: Jeon
authors.forename: Sungmo
authors.initials: S
authors.lastname: Je
authors.forename: Jinhee
authors.initials: J
authors.lastname: Park
authors.forename: Yeun
authors.initials: Y
authors.lastname: Kim
authors.forename: Eun-Gae
authors.initials: EG
authors.lastname: Lee
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Lee
authors.forename: Sangkyo
authors.initials: S
authors.lastname: Seo
authors.forename: Sang-Nae
authors.initials: SN
authors.lastname: Cho
mesh_terms: D000818:Animals; D002417:Cattle; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D005838:Genotype; D007723:Korea / Q000453:epidemiology; D018598:Minisatellite Repeats / Q000235:genetics*; D009163:Mycobacterium bovis / Q000235:genetics*; D015995:Prevalence; D014380:Tuberculosis, Bovine / Q000453:epidemiology* / Q000382:microbiology*
chemical_list: D017931:DNA Primers