Gene expression is reduced in trans by inosine-containing dsRNA.
Inosine residues may be introduced into long dsRNA (double-stranded RNA) molecules by the action of a family of editing enzymes, ADARs (adenosine deaminases that act on RNA). Furthermore, hyperediting of dsRNA by ADARs may result in up to 50% of adenosine residues being converted into inosine. While the effect of hyperediting has traditionally been thought to be limited to the edited dsRNA, we have recently shown that hyperedited dsRNA [I-dsRNA (inosine-containing dsRNA)] is able to down-regulate the expression of both reporter and endogenous mRNAs in cells, in trans. Down-regulation by I-dsRNA occurs both by reducing mRNA levels and by inhibiting of translation. This finding has important functional consequences for hyperediting by ADARs.
Scadden
A D J
AD
Department of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UK. adjs@mole.bio.cam.ac.uk
D001483:Base Sequence; D003594:Cytoplasmic Granules / Q000187:drug effects / Q000378:metabolism; D015536:Down-Regulation / Q000187:drug effects*; D006367:HeLa Cells; D006801:Humans; D007288:Inosine / Q000494:pharmacology*; D008957:Models, Genetic; D008969:Molecular Sequence Data; D014176:Protein Biosynthesis / Q000187:drug effects; D012330:RNA, Double-Stranded / Q000235:genetics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012330:RNA, Double-Stranded; D012333:RNA, Messenger; D007288:Inosine