A simple solid-phase synthesis of the ubiquitous bacterial signaling molecule, c-di-GMP and analogues.
Cyclic-di-guanylate (c-di-GMP) has emerged as a general and important signaling molecule uniquely present in bacteria: herein we provide a simple solid-phase synthesis of c-di-GMP using an automated DNA synthesizer for the majority of the synthesis.
Kiburu
Irene
I
Department of Chemistry and Biochemistry, Building 091, University of Maryland, College Park, MD 20742, USA. hsintim@umd.edu.
Shurer
Andrew
A
Yan
Lei
L
Sintim
Herman O
HO
D001419:Bacteria / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006152:Cyclic GMP / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D003500:Cyclization; D008968:Molecular Conformation; D015398:Signal Transduction; D013237:Stereoisomerism
C062025:bis(3',5')-cyclic diguanylic acid; D006152:Cyclic GMP