Protective role of exogenous nitric oxide against oxidative-stress induced by salt stress in barley (Hordeum vulgare).
To probe into the potential of relieving the oxidative damage of salt stress, we investigated the protective role of nitric oxide on barley under salt stress. Salt stress resulted in increased ion leakage, lipid peroxidation and protein oxidation in barley leaves. Simultaneous treatments of barley leaves with 50 microM sodium nitroprusside, a nitric oxide donor, alleviated the damage of salt stress, reflected by decreased ion leakage, and malendialdehyde (MDA), carbonyl, and hydrogen peroxide content in barley leaves. The presence of the nitric oxide donor increased the activities of superoxide dismutases (SOD), ascorbate peroxidases (APX), and catalases (CAT). Meantime, sodium nitroprusside addition increased accumulation of ferritin at the protein level, indicating that nitric oxide directly regulated ferritin accumulation. These results suggested that nitric oxide can effectively protect seedlings from salt stress damage by enhancing activities of antioxidant enzymes to quench the excessive reactive oxygen species caused by salt stress and inducing the increase of ferritin accumulation to chelate larger number of ferrous ion. Information from this study can be used to improve soil management practices for sustainable use of salt-affected soils in the future.
Li
Qiao-Yun
QY
School of Life Science and Technology, Shanxi University, Taiyuan 030006, China.
Niu
Hong-Bin
HB
Yin
Jun
J
Wang
Meng-Ben
MB
Shao
Hong-Bo
HB
Deng
De-Zhi
DZ
Chen
Xiao-Xia
XX
Ren
Jiang-Ping
JP
Li
Yong-Chun
YC
D004591:Electrophoresis, Polyacrylamide Gel; D001467:Hordeum / Q000502:physiology*; D009569:Nitric Oxide / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D012965:Sodium Chloride; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009569:Nitric Oxide; D012965:Sodium Chloride