Two new megastigmane glycosides, physanosides A and B, from Physalis alkekengi L. var. franchetii, and their effect on NO release in macrophages.
Two new megastigmane glycosides, physanosides A and B (1 and 2, resp.), were isolated from Physalis alkekengi L. var. franchetii, together with four known compounds (6S,9R)-roseoside (3), (6S,9S)-roseoside (4), (6R,9S)-3-oxo-alpha-ionol beta-D-glucopyranoside (5), and citroside A (6). Their structures were elucidated on the basis of physicochemical evidence, in-depth NMR spectroscopic analysis, high-resolution mass spectrometry, and CD spectroscopy, and their inhibitory effect on NO production was also examined. Compounds 2 and 3 exhibited strong inhibition on lipopolysaccharide-induced NO release by macrophages with IC(50) values of 9.93 and 7.31 microM, respectively.
Qiu
Li
L
Department of Natural Products Chemistry, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University 81#, No. 103 Road WenHua, Shenyang 110016, PR China.
Zhao
Feng
F
Liu
Hongwei
H
Chen
Lixia
L
Jiang
Zhihu
Z
Liu
Hongxia
H
Wang
Naili
N
Yao
Xinsheng
X
Qiu
Feng
F
D000818:Animals; D002460:Cell Line; D003512:Cyclohexanones / Q000737:chemistry* / Q000494:pharmacology*; D004187:Disaccharides / Q000737:chemistry* / Q000494:pharmacology*; D006027:Glycosides / Q000737:chemistry* / Q000494:pharmacology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000378:metabolism*; D032321:Physalis / Q000737:chemistry*
D003512:Cyclohexanones; D004187:Disaccharides; D006027:Glycosides; D008070:Lipopolysaccharides; C530763:physanoside A; D009569:Nitric Oxide