Hypertrophy of mature Xenopus muscle fibres in culture induced by synergy of albumin and insulin.
The aim of this study was to investigate effects of albumin and insulin separately as well as in combination on mature muscle fibres during long-term culture. Single muscle fibres were dissected from m. iliofibularis of Xenopus laevis and attached to a force transducer in a culture chamber. Fibres were cultured in a serum-free medium at slack length (mean sarcomere length 2.3 mum) for 8 to 22 days. The medium was supplemented with (final concentrations): (1) bovine insulin (6 nmol/L or 200-600 nmol/L), (2) 0.2% bovine albumin or (3) 0.2% bovine albumin in combination with insulin (120 nmol/L). In culture medium with insulin, 50% of the muscle fibres became in-excitable within 7-12 days, whereas the other 50% were stable. Caffeine contractures of in-excitable muscle fibres produced 80.4 +/- 2.4% of initial peak tetanic force, indicating impaired excitation-contraction (E-C) coupling in in-excitable fibres. In the presence of albumin, all cultured muscle fibres were stable for at least 10 days. Muscle fibres cultured in medium with insulin or albumin exclusively did not hypertrophy or change the number of sarcomeres in series. In contrast, muscle fibres cultured with both albumin and insulin showed an increase in tetanic force and fibre cross-sectional area of 19.6 +/- 2.8% and 32.5 +/- 4.9%, respectively, (means +/- SEM.; P = 0.007) after 16.3 +/- 1.7 days, whereas the number of sarcomeres in series remained unchanged. We conclude that albumin prevents muscle fibre damage and preserves E-C coupling in culture. Furthermore, albumin is important in regulating muscle fibre adaptation by a synergistic action with growth factors like insulin.
Jaspers
R T
RT
Research Institute Move, Faculty of Human Movement Sciences, VU University Amsterdam, van der Boechorststraat 9, 1081 BT, Amsterdam, The Netherlands. r.jaspers@fbw.vu.nl
van Beek-Harmsen
B J
BJ
Blankenstein
M A
MA
Goldspink
G
G
Huijing
P A
PA
van der Laarse
W J
WJ
D000251:Adenosine Triphosphatases / Q000378:metabolism; D000418:Albumins / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D003720:Densitometry; D015194:Diffusion Chambers, Culture; D005260:Female; D006003:Glycogen / Q000378:metabolism; D006984:Hypertrophy; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D007150:Immunohistochemistry; D007328:Insulin / Q000494:pharmacology*; D008055:Lipids / Q000737:chemistry; D008931:Mitochondria, Muscle / Q000187:drug effects / Q000201:enzymology; D009119:Muscle Contraction / Q000187:drug effects; D018485:Muscle Fibers, Skeletal / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D009210:Myofibrils / Q000187:drug effects / Q000201:enzymology; D012518:Sarcomeres / Q000187:drug effects / Q000648:ultrastructure; D014982:Xenopus laevis
D000418:Albumins; D007004:Hypoglycemic Agents; D007328:Insulin; D008055:Lipids; D006003:Glycogen; D000251:Adenosine Triphosphatases