Critical role of p53 in histone deacetylase inhibitor-induced Epstein-Barr virus Zta expression.
The tumor suppressor gene p53 plays a central role in the maintenance of normal cell growth and genetic integrity, while its impact on the Epstein-Barr virus (EBV) life cycle remains elusive. We found that p53 is important for histone deacetylase inhibitor-induced EBV lytic gene expression in nasopharyngeal carcinoma cells. Restoration of p53 in p53-null, EBV-infected H1299 cells augments the potential for viral lytic cycle initiation. Evidence from reporter assays demonstrated that p53 contributes to the expression of the immediate-early viral Zta gene. Further analysis indicated that the DNA-binding ability of p53 and phosphorylation of Ser392 may be critical. This study provides the first evidence that p53 is involved in the regulation of EBV lytic cycle initiation.
Chang
Shih-Shin
SS
Graduate Institute of Microbiology, College of Medicine, National Taiwan University, No. 1, Sec. 1, Ren-Ai Rd., Taipei 10051, Taiwan.
Lo
You-Chang
YC
Chua
Huey-Huey
HH
Chiu
Hsin-Yi
HY
Tsai
Shu-Chun
SC
Chen
Jen-Yang
JY
Lo
Kwok-Wai
KW
Tsai
Ching-Hwa
CH
D045744:Cell Line, Tumor; D005816:Genetic Complementation Test; D004854:Herpesvirus 4, Human / Q000502:physiology*; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D015534:Trans-Activators / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000378:metabolism*; D014775:Virus Activation
C063676:BZLF1 protein, Herpesvirus 4, Human; D056572:Histone Deacetylase Inhibitors; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53