Elevated serum level and gene polymorphisms of TGF-beta1 in gastric cancer.
Transforming growth factor (TGF)-beta1, as a candidate tumor marker, is currently of interest. In this study, serum TGF-beta1 levels in gastric cancer (GC) patients and healthy volunteers were measured using enzyme-linked immunosorbent assay (ELISA). In addition, single nucleotide polymorphisms (SNPs) of the TGF-beta1 gene at codon 10 and codon 25 were identified by means of amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) and sequence analysis. Our results indicated that serum concentrations of TGF-beta1 in GC patients were significantly higher than those in the control, and positively correlated with tumor mass, invasion, metastasis, and clinical stage. The serum TGF-beta1 levels of patients recovering from radical resection were markedly lower than those before surgery. Meanwhile, no deoxyribonucleic acid (DNA) sequence variation at codon 25 of the TGF-beta1 gene was found and a TGF-beta1 gene polymorphism at codon 10 did not show obvious correlations with either TGF-beta1 expression or clinicopathological parameters of GC. Our evidence suggested that serum concentration of TGF-beta1 might be a novel tumor marker for GC and the polymorphisms of TGF-beta1 gene did not play a role as a determinant of serum TGF-beta1 concentration or as a genetic risk factor in the gastric carcinogenesis and progression.
Li
Xue
X
Laboratory of Medical Genetics, Harbin Medical University, Harbin, China.
Yue
Zhi-Chao
ZC
Zhang
Yun-Yan
YY
Bai
Jing
J
Meng
Xiang-Ning
XN
Geng
Jing-Shu
JS
Fu
Song-Bin
SB
D000230:Adenocarcinoma / Q000097:blood* / Q000235:genetics / Q000556:secondary; D000328:Adult; D000368:Aged; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000097:blood; D004273:DNA, Neoplasm / Q000032:analysis; D018450:Disease Progression; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008198:Lymph Nodes / Q000473:pathology; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013274:Stomach Neoplasms / Q000097:blood* / Q000235:genetics / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000097:blood* / Q000235:genetics
D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm; D053773:Transforming Growth Factor beta1