Leishmania major: identification of developmentally regulated proteins in procyclic and metacyclic promastigotes.
The differentiation from procyclic to metacyclic promastigotes (metacyclogenesis) has been correlated with an increased infectivity in a number of Leishmania species. We compared the proteomes of procyclic and metacyclic promastigotes of L. major. Lysates from either life cycle stage were resolved by 2D-PAGE, followed by Coomassie brilliant blue staining. Spots were analyzed by MALDI-TOF MS. 25 protein spots were found to be differentially expressed during metacyclogenesis. We found that proteins involved in protein synthesis were less abundant in metacyclic promastigotes, while proteins involved in motility, including paraflagellar rod protein 1D, alpha-tubulin and beta-tubulin were more abundant. Also, two mitochondrial enzymes (succinyl-CoA synthetase beta subunit and cytochrome c oxidase subunit IV) were differentially expressed in both life cycle stages. Down-regulation of proteins related to synthetic pathway in metacyclic promastigotes is consistent with the arrested growth in this life cycle stage, while up-regulation of proteins related to motility in metacyclic promastigotes is in agreement with the high motility observed in this stage.
Mojtahedi
Zahra
Z
Department of Immunology, Shiraz University of Medical Sciences, Shiraz Medical School, P.O. Box 71345-1798, Shiraz 734-8553, Iran.
Clos
Joachim
J
Kamali-Sarvestani
Eskandar
E
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007091:Image Processing, Computer-Assisted; D007525:Isoelectric Focusing; D018320:Leishmania major / Q000737:chemistry / Q000254:growth & development* / Q000502:physiology; D016773:Leishmaniasis, Cutaneous / Q000469:parasitology; D008018:Life Cycle Stages / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009068:Movement / Q000502:physiology; D020543:Proteome / Q000737:chemistry*; D015800:Protozoan Proteins / Q000032:analysis* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome; D015800:Protozoan Proteins