Gelatin/PLLA sponge-like scaffolds allow proliferation and osteogenic differentiation of human mesenchymal stromal cells.
Tissue engineering has the potential to supply constructs capable of restoring the normal function of native tissue following injury. Poly(L-lactic acid) (PLLA) scaffolds are amongst the most commonly used biodegradable polymers in tissue engineering and previous studies performed on ovine fibroblasts have showed that addition of gelatin creates a favorable hydrophilic microenvironment for the growth of these cells. The attractiveness of using mesenchymal stromal cells (MSCs) in tissue regeneration is that they are able to differentiate into several lines including osteoblasts. In this study, we investigated the ability of gelatin/PLLA sponges to support the adhesion, proliferation, and osteogenic differentiation of human MSCs isolated from the bone marrow of four donors. [Figure: see text].
Mattii
Letizia
L
Department of Human Morphology and Applied Biology, Section of Histology and General Embryology, University of Pisa, 56126 Pisa, Italy. l.mattii@med.unipi.it
Battolla
Barbara
B
D'Alessandro
Delfo
D
Trombi
Luisa
L
Pacini
Simone
S
Cascone
Maria Grazia
MG
Lazzeri
Luigi
L
Bernardini
Nunzia
N
Dolfi
Amelio
A
Galimberti
Sara
S
Petrini
Mario
M
D002454:Cell Differentiation; D049109:Cell Proliferation; D005780:Gelatin; D006801:Humans; D019344:Lactic Acid; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis; D011091:Polyesters; D011108:Polymers; D017154:Stromal Cells / Q000166:cytology; D023822:Tissue Engineering / Q000379:methods*
D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); D005780:Gelatin