Contributions of nucleus accumbens core and shell GluR1 containing AMPA receptors in AMPA- and cocaine-primed reinstatement of cocaine-seeking behavior.
Glutamate signaling in the nucleus accumbens influences reinstatement of previously extinguished cocaine-seeking behavior in rats. Whether or not region specific glutamate signaling in the nucleus accumbens contributes to reinstatement of cocaine-seeking behavior is not known. We investigated whether directly stimulating ionotropic glutamate receptors (GluRs) within the nucleus accumbens core or shell would differentially influence renewed cocaine-seeking behavior following extinction training. We also tested the hypothesis that GluR1 subunit (GluR1) containing alpha-amino-3-hydroxy-5-methylisoxazole-4-proprionic acid (AMPA) receptors in the nucleus accumbens core and not the shell regulate reinstatement of previously extinguished cocaine-seeking behavior. Microinjection of AMPA into the nucleus accumbens shell and the nucleus accumbens core dose-dependently elicited significant cocaine-seeking behavior. Administration of antisense oligonucleotides (AS) directed against GluR1 subunit mRNA into the core and shell disrupted AMPA- and cocaine-primed reinstatement--with the most pronounced effects seen in the nucleus accumbens shell. These results demonstrate that GluRs in the nucleus accumbens core and shell influence AMPA- and cocaine-primed reinstatement, yet the nucleus accumbens shell exerts a prepotency over the nucleus accumbens core.
Ping
Ansong
A
Department of Physiology, CA 2098, Medical College of Georgia, 1120 15th Street, Augusta, Georgia 30912, USA.
Xi
Jinlei
J
Prasad
Balakrishna M
BM
Wang
Mong-Heng
MH
Kruzich
Paul J
PJ
D000818:Animals; D001070:Appetitive Behavior / Q000187:drug effects / Q000502:physiology*; D016739:Behavior, Addictive / Q000378:metabolism*; D003042:Cocaine / Q000008:administration & dosage; D019970:Cocaine-Related Disorders / Q000378:metabolism; D018765:Dopamine Uptake Inhibitors / Q000008:administration & dosage; D004305:Dose-Response Relationship, Drug; D018690:Excitatory Amino Acid Agonists / Q000008:administration & dosage; D008297:Male; D008845:Microinjections; D018377:Neurotransmitter Agents / Q000008:administration & dosage; D009714:Nucleus Accumbens / Q000033:anatomy & histology / Q000187:drug effects / Q000378:metabolism*; D020320:Oligoribonucleotides, Antisense / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D018091:Receptors, AMPA / Q000187:drug effects / Q000378:metabolism*; D012646:Self Administration; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000008:administration & dosage
D003042:Cocaine; D018765:Dopamine Uptake Inhibitors; D018690:Excitatory Amino Acid Agonists; D018377:Neurotransmitter Agents; D020320:Oligoribonucleotides, Antisense; D018091:Receptors, AMPA; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid