title: Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin.
abstract: We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.
authors.affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.
authors.forename: Domenica
authors.initials: D
authors.lastname: Mangieri
authors.forename: Beatrice
authors.initials: B
authors.lastname: Nico
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Benagiano
authors.forename: Michela
authors.initials: M
authors.lastname: De Giorgis
authors.forename: Angelo
authors.initials: A
authors.lastname: Vacca
authors.forename: Domenico
authors.initials: D
authors.lastname: Ribatti
mesh_terms: D000818:Animals; D002642:Chick Embryo; D049033:Chorioallantoic Membrane / Q000098:blood supply; D015536:Down-Regulation; D043169:Endostatins / Q000378:metabolism*; D042783:Endothelial Cells / Q000378:metabolism*; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D009101:Multiple Myeloma / Q000473:pathology*; D018919:Neovascularization, Physiologic / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D043169:Endostatins; D012333:RNA, Messenger; D016222:Fibroblast Growth Factor 2