Lumican induces human corneal epithelial cell migration and integrin expression via ERK 1/2 signaling.
Lumican is a major proteoglycans of the human cornea. Lumican knock-out mice have been shown to lose corneal transparency and to display delayed wound healing. The purpose of this study was to define the role of lumican in corneal epithelial cell migration. Over-expression of lumican in human corneal epithelial (HCE-T) cells increased both cell migration and proliferation, and increased levels of integrins alpha2 and beta1. ERK 1/2 was also activated in lumican over-expressed cells. When we treated HCE-T cells with the ERK-specific inhibitor U0126, cell migration and the expression of integrin beta1 were completely blocked. These data provide evidence that lumican stimulates cell migration in the corneal epithelium by activating ERK 1/2, and point to a novel signaling pathway implicated in corneal epithelial cell migration.
Seomun
Young
Y
Laboratory of Ophthalmology and Visual Science, Korean Eye Tissue and Gene Bank related to Blindness, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-ku, Seoul 137-040, Republic of Korea.
Joo
Choun-Ki
CK
D002070:Butadienes / Q000494:pharmacology; D002460:Cell Line; D002465:Cell Movement; D011508:Chondroitin Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism*; D019573:Epithelium, Corneal / Q000166:cytology* / Q000502:physiology*; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics / Q000378:metabolism; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism*; D007632:Keratan Sulfate / Q000235:genetics / Q000378:metabolism*; D000071234:Lumican; D019950:Mitogen-Activated Protein Kinase 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D009570:Nitriles / Q000494:pharmacology; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D015398:Signal Transduction
D002070:Butadienes; D011508:Chondroitin Sulfate Proteoglycans; D039421:Integrin alpha2; D019012:Integrin beta1; C000604306:LUM protein, human; C000604307:Lum protein, mouse; D000071234:Lumican; D009570:Nitriles; D047428:Protein Kinase Inhibitors; C113580:U 0126; D007632:Keratan Sulfate; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3