title: Regulation of electrolyte transport with IL-1beta in rabbit distal colon.
abstract: Interletrkin-1beta levels are elevated in inflammatory bowel disease. In this study the mechanism by which interleukin-1beta affects electrolyte transport in the rabbit distal colon, was investigated. Interleukin-1beta caused a delayed increase in short-circuit current (I(sc)) which was attributed to protein synthesis since the effect was inhibited by cycloheximide. The interleukin-1beta induced increase in I(sc) was not affected by amiloride treatment but was completely inhibited by bumetanide or in chloride-free buffer and by indomethacin. Prostaglandin E(2) levels increased in tissue treated with interleukin-1beta, but this increase was reversed by cycloheximide. These data suggest that interleukin-1beta causes its effect via a yet to be identified second messenger, by increasing chloride secretion through a prostaglandin E(2) mediated mechanism.
authors.affiliation: Division of Gastroenterology and Nutrition Winthrop-University Hospital Mineola NY 11501 USA.
authors.forename: F R
authors.initials: FR
authors.lastname: Homaidan
authors.forename: H
authors.initials: H
authors.lastname: Desai
authors.forename: L
authors.initials: L
authors.lastname: Zhao
authors.forename: G
authors.initials: G
authors.lastname: Broutman
authors.forename: R
authors.initials: R
authors.lastname: Burakoff