title: Mind bomb 1-expressing intermediate progenitors generate notch signaling to maintain radial glial cells.
abstract: Notch signaling is critical for the stemness of radial glial cells (RGCs) during embryonic neurogenesis. Although Notch-signal-receiving events in RGCs have been well characterized, the signal-sending mechanism by the adjacent cells is poorly understood. Here, we report that conditional inactivation of mind bomb-1 (mib1), an essential component for Notch ligand endocytosis, in mice using the nestin and hGFAP promoters resulted in complete loss of Notch activation, which leads to depletion of RGCs, and premature differentiation into intermediate progenitors (IPs) and finally neurons, which were reverted by the introduction of active Notch1. Interestingly, Mib1 expression is restricted in the migrating IPs and newborn neurons, but not in RGCs. Moreover, sorted Mib1+ IPs and neurons can send the Notch signal to neighboring cells. Our results reveal that not only newborn neurons but also IPs are essential Notch-ligand-presenting cells for maintaining RGC stemness during both symmetric and asymmetric divisions.
authors.affiliation: Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Kyungbuk, 790-784, South Korea.
authors.forename: Ki-Jun
authors.initials: KJ
authors.lastname: Yoon
authors.forename: Bon-Kyoung
authors.initials: BK
authors.lastname: Koo
authors.forename: Sun-Kyoung
authors.initials: SK
authors.lastname: Im
authors.forename: Hyun-Woo
authors.initials: HW
authors.lastname: Jeong
authors.forename: Jaewang
authors.initials: J
authors.lastname: Ghim
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Kwon
authors.forename: Jin-Sook
authors.initials: JS
authors.lastname: Moon
authors.forename: Takaki
authors.initials: T
authors.lastname: Miyata
authors.forename: Young-Yun
authors.initials: YY
authors.lastname: Kong
mesh_terms: D000367:Age Factors; D000818:Animals; D002454:Cell Differentiation / Q000502:physiology; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics; D049452:Green Fluorescent Proteins; D007381:Intermediate Filament Proteins / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008954:Models, Biological; D009410:Nerve Degeneration / Q000235:genetics; D009419:Nerve Tissue Proteins / Q000378:metabolism; D064231:Nestin; D009457:Neuroglia / Q000502:physiology*; D009474:Neurons / Q000502:physiology; D009924:Organ Culture Techniques / Q000379:methods; D016548:Prosencephalon / Q000166:cytology; D051880:Receptors, Notch / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*; D013997:Time Factors; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000502:physiology*
chemical_list: D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin; D051880:Receptors, Notch; D049452:Green Fluorescent Proteins; C470629:MIB1 protein, mouse; D044767:Ubiquitin-Protein Ligases