Esco2 is a novel corepressor that associates with various chromatin modifying enzymes.
Accurate chromosome segregation during cell division requires that sister chromatids are kept together by cohesin complex until anaphase, when the chromatids separate and distribute to the two daughter cells. Esco2 is an acetyltransferase that is required for the establishment of sister chromatid cohesion during S phase. Here, we report that Esco2 interacts with several component proteins of the CoREST complex, including a transcription corepressor CoREST, histone demethlyase LSD1, HDAC1, HDAC2, BRAF35, and PHF21A. Esco2 also interacts with various histone methyltransferases Suv39h1, SETDB1 and G9a. Esco2 complex purified from HeLa nuclear extract possesses histone H3 K9 methylation activity and functions as a transcription repressor. Esco2 fused to Gal4 DNA binding domain represses transcription by increasing methylation of histone H3 K9 in the promoter region. These results suggest a novel function of Esco2 in transcription repression through modulation of the chromatin structure.
Kim
Beom-Jun
BJ
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300 Chunchun-dong, Jangan-gu, Suwon, Kyonggi-do 440-746, Republic of Korea.
Kang
Kyung-Min
KM
Jung
Sung Yun
SY
Choi
Hyun-Kyung
HK
Seo
Jong-Hun
JH
Chae
Ji-Hye
JH
Cho
Eun-Jung
EJ
Youn
Hong-Duk
HD
Qin
Jun
J
Kim
Seong-Tae
ST
D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002843:Chromatin / Q000201:enzymology*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D008780:Methyltransferases / Q000378:metabolism; D011496:Protein Methyltransferases / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D012097:Repressor Proteins; C407843:SUV39H1 protein, human; D000076983:Histone Methyltransferases; D008780:Methyltransferases; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C454062:SETDB1 protein, human; D000123:Acetyltransferases; C500785:ESCO2 protein, human