title: The role of myosin II in glioma invasion of the brain.
abstract: The ability of gliomas to invade the brain limits the efficacy of standard therapies. In this study, we have examined glioma migration in living brain tissue by using two novel in vivo model systems. Within the brain, glioma cells migrate like nontransformed, neural progenitor cells-extending a prominent leading cytoplasmic process followed by a burst of forward movement by the cell body that requires myosin II. In contrast, on a two-dimensional surface, glioma cells migrate more like fibroblasts, and they do not require myosin II to move. To explain this phenomenon, we studied glioma migration through a series of synthetic membranes with defined pore sizes. Our results demonstrate that the A and B isoforms of myosin II are specifically required when a glioma cell has to squeeze through pores smaller than its nuclear diameter. They support a model in which the neural progenitor-like mode of glioma invasion and the requirement for myosin II represent an adaptation needed to move within the brain, which has a submicrometer effective pore size. Furthermore, the absolute requirement for myosin II in brain invasion underscores the importance of this molecular motor as a potential target for new anti-invasive therapies to treat malignant brain tumors.
authors.affiliation: Department of Neurology, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
authors.forename: Christopher
authors.initials: C
authors.lastname: Beadle
authors.forename: Marcela C
authors.initials: MC
authors.lastname: Assanah
authors.forename: Pascale
authors.initials: P
authors.lastname: Monzo
authors.forename: Richard
authors.initials: R
authors.lastname: Vallee
authors.forename: Steven S
authors.initials: SS
authors.lastname: Rosenfeld
authors.forename: Peter
authors.initials: P
authors.lastname: Canoll
mesh_terms: D000818:Animals; D001921:Brain / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism*; D002465:Cell Movement; D015180:Electrophoresis, Gel, Two-Dimensional; D005347:Fibroblasts / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D005910:Glioma / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D024462:Myosin Type II / Q000737:chemistry / Q000502:physiology*; D009368:Neoplasm Transplantation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D049452:Green Fluorescent Proteins; D024462:Myosin Type II