Elimination of matrix effects in the determination of bisphenol A in milk by solid-phase microextraction-high-performance liquid chromatography.
Solid-phase microextraction coupled to high-performance liquid chromatography (SPME-HPLC) with fluorescence detection was employed to determine bisphenol A (BPA) in milk samples. The potential influence of the milk matrix on the determination of BPA by SPME-HPLC were investigated. Optimal conditions to eliminate any matrix effects were as follows: milk samples were deproteinized with trichloroacetic acid, diluted 20-fold with BPA-free Ultrapure water, dissolved in methanol, the precipitated protein was filtered out, rinsed with methanol and evaporated to remove the methanol. Then, a 40.0-ml solution was used for SPME extraction and HPLC analysis. Satisfactory recoveries (milk: 93.1-101%; soybean milk: 93.9-102%) were achieved. The proposed method was successfully applied to real samples, BPA being detected within the range 1.6-2.6 ng ml(-1) in four brands of commercial milk but not in soybean milk.
Liu
Xiaoyan
X
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Ji
Yongsheng
Y
Zhang
Haixia
H
Liu
Mancang
M
D000042:Absorption; D000818:Animals; D001559:Benzhydryl Compounds; D002364:Caseins; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis*; D008892:Milk / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods; D041942:Soy Milk / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D002364:Caseins; D010636:Phenols; D014874:Water Pollutants, Chemical; C006780:bisphenol A; D000098826:Bisphenol A Compounds