title: A quick and reliable method for monitoring gene expression in actinomycetes.
abstract: Amycolatopsis balhimycina DSM5908 is the producer of the vancomycin-type glycopeptide antibiotic balhimycin. Balhimycin production is controlled by transcriptional regulators and depends on different environmental factors. To analyse the regulatory network P(tba), a representative promoter of the balhimycin gene cluster, was fused to the reporter gene egfp and integrated into the genome of A. balhimycina. Balhimycin production, fluorescence intensity and the amount of tba-transcript were determined under different cultivation conditions in microtiter plates. Thus we could demonstrate that these values are directly correlated. This approach facilitates the analysis of a large number of samples in parallel and it provides the opportunity to easily define conditions to increase balhimycin production.
authors.affiliation: Lehrstuhl für Mikrobiologie/Biotechnologie, Mikrobiologisches Institut, Universität Tübingen, Germany.
authors.forename: Meike
authors.initials: M
authors.lastname: Chevillotte
authors.forename: Rainer
authors.initials: R
authors.lastname: Menges
authors.forename: Günther
authors.initials: G
authors.lastname: Muth
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Wohlleben
authors.forename: Evi
authors.initials: E
authors.lastname: Stegmann
mesh_terms: D039903:Actinobacteria / Q000235:genetics*; D001709:Biotechnology / Q000379:methods*; D004269:DNA, Bacterial / Q000378:metabolism; D005453:Fluorescence; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics*; D009876:Operon / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D014640:Vancomycin / Q000031:analogs & derivatives / Q000096:biosynthesis
chemical_list: D004269:DNA, Bacterial; C087127:balhimycin; D014640:Vancomycin