title: Flow-injection chemiluminescence determination of enrofloxacin using the Ru(phen)3(2+)-Ce(IV) system and central composite design for the optimization of chemical variables.
abstract: The main purpose of this study was to develop an inexpensive, simple, rapid and sensitive chemiluminescence (CL) method for the determination of enrofloxacin (ENRO) using a flow-injection system. This method is based on rapid reduction of Ru(phen)(3)(3+), which is produced in the reaction between Ru(phen)(3)(2+) and acidic Ce(IV) by ENRO, producing strong CL. A central composite design (CCD) was used for optimization of the chemical variables. Regression analysis of the data from the CCD demonstrated that a second-order polynomial model is an adequate description of the surface over the factor limits studied. Optimization using CCD gave approximately four-fold better results than the single-factor-at-a-time method. Under optimal experimental conditions, the CL response was proportional to the concentration of ENRO over a wide range (0.008-3.6 microg/mL) with a correlation coefficient of 0.9986 and a detection limit of 0.003 microg/mL (3sigma). The relative standard deviation for 11 repeated determinations of 0.14 microg/mL ENRO was 4.2%. This method was successfully applied to the analysis of commercial formulations, spiked plasma and spiked poultry tissue. Sample analyses showed good recovery percentages for drugs and spiked plasma (95.1-103.9%). Recovery percentages for spiked poultry tissue were in the range 77.6-87.3%. The minimum sampling rate was 100 samples/h.
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156 IR, Iran. rezaei@cc.iut.ac.ir
authors.forename: B
authors.initials: B
authors.lastname: Rezaei
authors.forename: A
authors.initials: A
authors.lastname: Mokhtari
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis* / Q000097:blood; D001774:Blood Chemical Analysis; D002563:Cerium / Q000737:chemistry*; D000077422:Enrofloxacin; D017022:Flow Injection Analysis / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D024841:Fluoroquinolones / Q000032:analysis* / Q000097:blood; D008163:Luminescent Measurements / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D008460:Meat / Q000032:analysis; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D010618:Phenanthrolines / Q000737:chemistry*; D011200:Poultry; D012044:Regression Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D000970:Antineoplastic Agents; D024841:Fluoroquinolones; D009942:Organometallic Compounds; D010618:Phenanthrolines; C034282:tris-(1,10-phenanthroline)ruthenium; D002563:Cerium; D000077422:Enrofloxacin