Membrane-bound pyrroloquinoline quinone-dependent dehydrogenase in Gluconobacter oxydans M5, responsible for production of 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose.
A membrane-bound protein purified from Gluconobacter oxydans M5 was confirmed to be a pyrroloquinoline quinone-dependent D-sorbitol dehydrogenase. Gene disruption and complementation experiments demonstrated that this enzyme is responsible for the oxidation of 1-(2-hydroxyethyl) amino-1-deoxy-D-sorbitol (1NSL) to 6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose (6NSE), which is the precursor of an antidiabetic drug, miglitol.
Yang
Xue-Peng
XP
State Key Laboratory of Bioreactor Engineering, East China University of Science and Biotechnology, Mei-Long 130, Shanghai 200237, China.
Wei
Liu-Jing
LJ
Lin
Jin-Ping
JP
Yin
Bo
B
Wei
Dong-Zhi
DZ
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000378:metabolism*; D005816:Genetic Complementation Test; D005822:Genetic Vectors; D020600:Gluconobacter oxydans / Q000201:enzymology* / Q000235:genetics; D007064:L-Iditol 2-Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism; D010084:Oxidation-Reduction; D045542:PQQ Cofactor / Q000378:metabolism*; D010957:Plasmids; D013012:Sorbitol / Q000378:metabolism; D013013:Sorbose / Q000031:analogs & derivatives* / Q000096:biosynthesis*
C531438:6-(2-hydroxyethyl) amino-6-deoxy-L-sorbose; D001426:Bacterial Proteins; D008565:Membrane Proteins; D013012:Sorbitol; D045542:PQQ Cofactor; D007064:L-Iditol 2-Dehydrogenase; D013013:Sorbose