[Effect of inhibitor HNP1 transfection on tumor growth of human nasopharyngeal carcinoma cell line HNE-1].
OBJECTIVE
The aim of this study is to investigate the effects of inhibitor HNP1 transfection on proliferation and tumor growth of human nasopharyngeal carcinoma cell line HNE1.


METHODS
HNE1 cells were transfected with HNP1 by liposome, and the cytotoxic effect was detected by MTT, In vivo tumor growth test was performed in nude mice inoculated with transfected HNE1 cells. The therapeutic effect of HNP1 was evaluated in the inoculated tumors, alpha-defensin 1 expression was detected in implanted tumor tissues by immunohistochemical stain.


RESULTS
HNP1 transfection significantly inhibited the proliferation of HNE1 cells. MTT assay confirmed cytotoxic effect. In vivo study showed tumor volume was significantly smaller in HNP1 transfection group than that in control group (P < 0.01). Immunohistochemical stain showed alpha-defensin expression was increased in HNP1 transfection group.


CONCLUSIONS
HNP1 transfection can inhibit the proliferation of HNE1 cells, as well as tumor growth.
Yang
Hongbin
H
Department of Otolaryngology, West China Hospital, Sichuan University, Chengdu, 610041, China. hongbiny@163.com
Qin
Gang
G
Li
Zumao
Z
Xu
Ning
N
Wen
Yanjun
Y
Liang
Chuanyu
C
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D005260:Female; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D014162:Transfection; D047368:Tumor Burden; D023084:alpha-Defensins / Q000235:genetics*
D023084:alpha-Defensins