Pulpal and periapical response of dogs' teeth after pulpotomy and use of recombinant human bone morphogenetic protein-7 as a capping agent.
OBJECTIVE
The purpose of this study was to evaluate the pulpal and periapical response of dogs' teeth after pulpotomy and the use of recombinant human bone morphogenetic protein-7 as a capping agent.


METHODS
Pulpotomy was performed in 60 teeth from 6 dogs, and the remaining pulp tissue was capped with the following materials: (a) groups 1 and 5: recombinant human bone morphogenetic protein-7 associated with recombinant human-like collagen; (b) groups 2 and 6: recombinant human-like collagen; (c) groups 3 and 7: calcium hydroxide; and (d) groups 4 and 8: zinc oxide and eugenol cement. After 7 days (groups 1-4) and 70 days (groups 5-8), the animals were sacrificed and the pieces containing the teeth were removed and processed for histological analysis.


RESULTS
Seven-day period: according to the type of inflammatory infiltrate observed in the root pulp tissue: (a) groups 1 and 2 were classified as severe with intense vascular proliferation; (b) group 4 was classified as moderate; and (c) group 3 was classified as mild. No dentin bridge formation was observed in any specimen, and the periapical region had normal appearance. 70-day period: groups 5, 6, and 8 showed no dentin bridge formation, and the remaining pulp tissue presented necrotic areas with inflammatory cells in the periapical region as well as bone and cemental resorption. In group 7, there was dentin bridge formation, absence of inflammation, and absence of mineralized tissue resorption.


CONCLUSIONS
The use of rHuBMP-7/rHuCollagen as a capping material after pulpotomy did not show either satisfactory apical and periapical response or capacity of inducing deposition of mineralized tissue.
da Silva
Léa Assed Bezerra
LA
Department of Pediatric Clinics, Preventive and Social Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
de Paula e Silva
Francisco Wanderley Garcia
FW
Leonardo
Mário Roberto
MR
Assed
Sada
S
D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D003094:Collagen / Q000494:pharmacology; D003782:Dental Pulp / Q000187:drug effects*; D003785:Dental Pulp Capping / Q000379:methods*; D003790:Dental Pulp Necrosis / Q000139:chemically induced; D003809:Dentin, Secondary / Q000378:metabolism; D004285:Dogs; D005260:Female; D006801:Humans; D008297:Male; D010486:Periapical Tissue / Q000187:drug effects*; D011672:Pulpotomy / Q000379:methods*; D011994:Recombinant Proteins / Q000494:pharmacology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D015035:Zinc Oxide-Eugenol Cement / Q000494:pharmacology
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D011994:Recombinant Proteins; D016212:Transforming Growth Factor beta; D015035:Zinc Oxide-Eugenol Cement; D003094:Collagen; D002126:Calcium Hydroxide