title: Detection of 14-3-3zeta in cerebrospinal fluid following experimentally evoked seizures.
abstract: Surrogate and peripheral (bio)markers of neuronal injury may be of value in assessing effects of seizures on the brain or epilepsy development following trauma. The presence of 14-3-3 isoforms in cerebrospinal fluid (CSF) is a diagnostic indicator of Creutzfeldt-Jakob disease but these proteins may also be present following acute neurological insults. Here, we examined neuronal and 14-3-3 proteins in CSF from rats after seizures. Seizures induced by intra-amygdala microinjection of 0.1 microg kainic acid (KA) caused damage which was mainly restricted to the ipsilateral CA3 subfield of the hippocampus. 14-3-3zeta was detected at significant levels in CSF sampled 4 h after seizures compared with near absence in control CSF. Neuron-specific nuclear protein (NeuN) was also elevated in CSF in seizure rats. CSF 14-3-3zeta levels were significantly lower in rats treated with 0.01 microg KA. These data suggest the presence of 14-3-3zeta within CSF may be a biomarker of acute seizure damage.
authors.affiliation: Department of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin, Ireland.
authors.forename: Niamh
authors.initials: N
authors.lastname: Murphy
authors.forename: Akitaka
authors.initials: A
authors.lastname: Yamamoto
authors.forename: David C
authors.initials: DC
authors.lastname: Henshall
mesh_terms: D048948:14-3-3 Proteins / Q000134:cerebrospinal fluid*; D000818:Animals; D015415:Biomarkers / Q000134:cerebrospinal fluid; D006624:Hippocampus / Q000473:pathology; D007608:Kainic Acid; D008297:Male; D009419:Nerve Tissue Proteins / Q000134:cerebrospinal fluid; D051381:Rats; D017207:Rats, Sprague-Dawley; D012640:Seizures / Q000134:cerebrospinal fluid* / Q000139:chemically induced / Q000473:pathology
chemical_list: D048948:14-3-3 Proteins; D015415:Biomarkers; D009419:Nerve Tissue Proteins; D007608:Kainic Acid