Acid adaptation of Lactobacillus delbrueckii subsp. bulgaricus induces physiological responses at membrane and cytosolic levels that improves cryotolerance.
OBJECTIVE
This work aimed at clarifying the physiological responses of Lactobacillus delbrueckii subsp. bulgaricus CFL1 cells after exposure to acidification at the end of fermentation, in relation to their cryotolerance.


RESULTS
Cells acidified at the end of the fermentation (pH 5.25 for 30 min) had their cryotolerance improved as compared to the reference condition (pH 6.0). By analyzing the cytosolic proteome, it was established that changes occurred in the synthesis of 21 proteins, involved in energy metabolism, nucleotide and protein synthesis and stress response. Acidification also induced a slight decrease in unsaturated to saturated and cyclic to saturated membrane fatty acid ratios.


CONCLUSIONS
Lactobacillus bulgaricus CFL1 was able to develop a combined physiological response at both membrane and cytosolic levels. This acid adaptation was referred as a cross-protection phenomenon as it allowed the cells to become more tolerant to cold stress.


CONCLUSIONS
This study increased knowledge concerning the physiological mechanisms that explained the cross-protection by acid adaptation. It may be useful for improving cryotolerance of lactic acid bacteria, either in cells banks or in an industrial context.
Streit
F
F
UMR782 Génie et Microbiologie des Procédés Alimentaires, AgroParisTech, INRA CBAI, Thiverval-Grignon, France.
Delettre
J
J
Corrieu
G
G
Béal
C
C
D000222:Adaptation, Physiological; D002462:Cell Membrane / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D005227:Fatty Acids / Q000032:analysis; D005285:Fermentation; D005516:Food Microbiology; D005615:Freezing; D006863:Hydrogen-Ion Concentration; D048668:Lactobacillus delbrueckii / Q000378:metabolism / Q000502:physiology*; D050296:Microbial Viability; D019936:Probiotics; D040901:Proteomics; D015014:Yogurt
D005227:Fatty Acids