A cyclic RGD-coated peptide nanoribbon as a selective intracellular nanocarrier.
We have synthesized a peptide-based supramolecular building block consisting of a cyclic Arg-Gly-Asp (cRGD) peptide segment and a beta-sheet-forming peptide segment. The block peptide was shown to self-assemble into a cRGD-coated nanoribbon structure, as revealed by circular dichroism (CD), dynamic light scattering (DLS), and transmission electron microscopy (TEM) studies. We have shown that this cRGD-coated nanoribbon can encapsulate hydrophobic guest molecules and deliver them into cells. Colocalization of the nanoribbon with LysoTracker and the selective intracellular delivery results suggests that the cRGD-coated nanoribbon is likely to be internalized into the cells through integrin receptors.
Lim
Yong-Beom
YB
Centre for Supramolecular Nano-Assembly, Department of Chemistry, Yonsei University, Shinchon 134, Seoul 120-749, Korea.
Kwon
Oh-Joon
OJ
Lee
Eunji
E
Kim
Pyung-Hwan
PH
Yun
Chae-Ok
CO
Lee
Myongsoo
M
D000595:Amino Acid Sequence; D002942:Circular Dichroism; D006367:HeLa Cells; D006801:Humans; D019169:Jurkat Cells; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D049329:Nanostructures; D009842:Oligopeptides / Q000737:chemistry*; D010456:Peptides, Cyclic / Q000737:chemistry*
D009842:Oligopeptides; D010456:Peptides, Cyclic; C047981:arginyl-glycyl-aspartic acid