title: [CDR3 spectratyping and clonal expansion of peripheral blood T cells in a patient with beta-mediterranean anaemia following peripheral blood stem cell transplantation].
abstract: OBJECTIVE
  To study the CDR3 spectratyping and clonal expansion of T cells in the peripheral blood mononuclear cells (PBMCs) of patients with beta-mediterranean anemia patient undergoing allogeneic peripheral blood stem cell (PBSC) transplantation.
  
  
  METHODS
  The total RNA was isolated from the PBMCs of a nine-year-old boy with beta-mediterranean anemia before and after PBSC transplantation, and at the time of occurrence of graft-versus-host disease (GVHD). The CDR3 length was analyzed using immunoscope technique, and the characteristics of the T cell receptor (TCR) on the T cells with clonal expansion were examined by gene sequencing.
  
  
  RESULTS
  The 24 TCR BV CDR3 repertoire showed Gaussian distribution in the PBMCs isolated before the transplantation, and some of the TCR BV family CDR3 showed skewing in the PBMCs isolated 23 days after transplantation and at the onset of GVHD (28 days after transplantation), suggesting the clonal expansion of the donor PBSCs.
  
  
  CONCLUSIONS
  The host PBMCs show muti-clonal expansion 23 days after PBSC transplantation, and in the stage of GVHD, some of the TCR BV family T cells show significant monoclonal expansion. Analysis of TCR CDR3 spectratyping and the molecular characteristics of specific TCR may help evaluate the immune reconstitution following the transplantation and indicate specific treatment for potential GVHD.
authors.affiliation: Institute of Molecular Immunology, School of Biotechnology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
authors.forename: Xin-sheng
authors.initials: XS
authors.lastname: Yao
authors.forename: Ming-qian
authors.initials: MQ
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Ma
authors.forename: Wei
authors.initials: W
authors.lastname: Luo
authors.forename: Xiao-qin
authors.initials: XQ
authors.lastname: Feng
authors.forename: Qian
authors.initials: Q
authors.lastname: Wen
authors.forename: Xiao-ning
authors.initials: XN
authors.lastname: Wang
mesh_terms: D002648:Child; D022801:Complementarity Determining Regions / Q000235:genetics / Q000276:immunology*; D006086:Graft vs Host Disease / Q000209:etiology / Q000276:immunology*; D006801:Humans; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D017086:beta-Thalassemia / Q000276:immunology* / Q000601:surgery
chemical_list: D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta