title: [An analysis on chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization].
abstract: OBJECTIVE
  To analyze the numerical aberration of chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization.
  
  
  METHODS
  The experiment included 10 asthenospermia patients and 5 healthy men with normal semen quality as controls. Fluorescence in situ hybridization (FISH) and probes for chromosomes including X, Y and 18 were used to determine the frequency of the aneuploid of the chromosomes in spermatozoa.
  
  
  RESULTS
  Of the 45,547 spermatozoa counted from the semen samples, the hybridization rate was 99.18%. The frequencies of the chromosome disomies including XX18, XY18, YY18, X1818 and Y1818 were (0.124 +/- -0.086)%, (0.360 +/- 0.380)%, (0.109 +/- 0.195)%, (0.342 +/- 0.746)% and (0.299 +/- 0.564)% in the case group and (0.014 +/- 0.019)%, (0.090 +/- 0.080)%, (0.030 +/- 0.031)%, (0.068 +/- 0.103)% and (0.075 +/- 0.083)% in the control. The sperm aneuploid rate was 9.25% in the former and 2.70% in the latter, with significant difference in between (P< 0.01).
  
  
  CONCLUSIONS
  Asthenospermia patients have a higher aneuploid rate of sperm chromosome than normal fertile men. However, larger samples are yet to be studied to obtain more scientific evidence.
authors.affiliation: Chongqing Institute of Science and Technology for National Population and Family Planning, Chongqing 400020, China.
authors.forename: Lian-Bing
authors.initials: LB
authors.lastname: Li
authors.forename: Yan-Kai
authors.initials: YK
authors.lastname: Xia
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Li
authors.forename: Jing
authors.initials: J
authors.lastname: Lü
authors.forename: Ming-Fu
authors.initials: MF
authors.lastname: Ma
authors.forename: Ling
authors.initials: L
authors.lastname: Song
authors.forename: Yuan
authors.initials: Y
authors.lastname: Ji
authors.forename: Ji-Gao
authors.initials: JG
authors.lastname: Yang
authors.forename: Tian-Feng
authors.initials: TF
authors.lastname: Zhang
authors.forename: Xiao-Xing
authors.initials: XX
authors.lastname: Chen
authors.forename: Ying
authors.initials: Y
authors.lastname: Rong
authors.forename: Le-Tian
authors.initials: LT
authors.lastname: Zhao
mesh_terms: D000782:Aneuploidy; D053627:Asthenozoospermia / Q000235:genetics*; D020223:Chromosome Painting / Q000379:methods*; D002887:Chromosomes, Human, Pair 18; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D006801:Humans; D008297:Male; D012729:Sex Chromosome Aberrations; D013094:Spermatozoa / Q000378:metabolism