Genetic diversity and genotype multiplicity of Plasmodium falciparum infections in symptomatic individuals living in Bangui (CAR).
This study provides the first estimate of the genetic diversity and genotype multiplicity of Plasmodium falciparum infections in symptomatic individuals living in Bangui (Central African Republic, CAR). Three hundred thirty six clinical isolates were used for analysis of parasite population polymorphism and genotyped by nested-PCR of msp-1 block 2, and msp-2 block 3. We found a very high level of polymorphism, with, respectively, 17 and 25 different alleles at the msp-1 and msp-2 loci and a high percentage of multiclonal infections (42.7% with msp-1 and 76.7% with msp-2), with a mean of 1.7 genotype with msp-1 and 2.8 with msp-2. We observed that (i) multiclonal infections and allelic polymorphism of msp-2 were significantly more frequent in Southern districts than in Northern districts of Bangui suggesting that the epidemiological features of P. falciparum may vary within Bangui and (ii) showed that immunocompromised HIV-positive patients tend to have a lower average number of msp-2 allele per isolate than immunocompetent patients.
Dolmazon
Virginie
V
Institut Pasteur de Bangui, BP 923, Bangui, Central African Republic.
Matsika-Claquin
Marcelle Diane
MD
Manirakiza
Alexandre
A
Yapou
Ferdinand
F
Nambot
Marina
M
Menard
Didier
D
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D000818:Animals; D000953:Antigens, Protozoan / Q000235:genetics; D002488:Central African Republic / Q000453:epidemiology; D002648:Child; D002675:Child, Preschool; D005260:Female; D014644:Genetic Variation; D005838:Genotype; D015658:HIV Infections / Q000150:complications; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D016778:Malaria, Falciparum / Q000453:epidemiology / Q000469:parasitology*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000235:genetics; D008875:Middle Aged; D010963:Plasmodium falciparum / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D015800:Protozoan Proteins / Q000235:genetics
D000953:Antigens, Protozoan; D020066:Merozoite Surface Protein 1; D015800:Protozoan Proteins; C065974:merozoite surface protein 2, Plasmodium