Mutational analysis of the zinc- and substrate-binding sites in the CphA metallo-beta-lactamase from Aeromonas hydrophila.
The subclass B2 CphA (Carbapenemase hydrolysing Aeromonas) beta-lactamase from Aeromonas hydrophila is a Zn(2+)-containing enzyme that specifically hydrolyses carbapenems. In an effort to evaluate residues potentially involved in metal binding and/or catalysis (His(118), Asp(120), His(196) and His(263)) and in substrate specificity (Val(67), Thr(157), Lys(224) and Lys(226)), site-directed mutants of CphA were generated and characterized. Our results confirm that the first zinc ion is in interaction with Asp(120) and His(263), and thus is located in the 'cysteine' zinc-binding site. His(118) and His(196) residues seem to be interacting with the second zinc ion, as their replacement by alanine residues has a negative effect on the affinity for this second metal ion. Val(67) plays a significant role in the binding of biapenem and benzylpenicillin. The properties of a mutant with a five residue (LFKHV) insertion just after Val(67) also reveals the importance of this region for substrate binding. This latter mutant has a higher affinity for the second zinc ion than wild-type CphA. The T157A mutant exhibits a significantly modified activity spectrum. Analysis of the K224Q and N116H/N220G/K224Q mutants suggests a significant role for Lys(224) in the binding of substrate. Lys(226) is not essential for the binding and hydrolysis of substrates. Thus the present paper helps to elucidate the position of the second zinc ion, which was controversial, and to identify residues important for substrate binding.
Bebrone
Carine
C
Centre d'Ingénierie des Protéines, Université de Liège, Allée de 6 Août B6, Sart-Tilman 4000 Liège, Belgium.
Anne
Christine
C
Kerff
Frédéric
F
Garau
Gianpiero
G
De Vriendt
Kris
K
Lantin
Raphaël
R
Devreese
Bart
B
Van Beeumen
Jozef
J
Dideberg
Otto
O
Frère
Jean-Marie
JM
Galleni
Moreno
M
D016980:Aeromonas hydrophila / Q000201:enzymology* / Q000235:genetics*; D019943:Amino Acid Substitution / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites / Q000235:genetics; D004252:DNA Mutational Analysis; D004269:DNA, Bacterial / Q000032:analysis / Q000737:chemistry; D016297:Mutagenesis, Site-Directed; D013379:Substrate Specificity / Q000235:genetics; D015032:Zinc / Q000737:chemistry* / Q000378:metabolism; D001618:beta-Lactamases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D001618:beta-Lactamases; C063912:carbapenemase; C082063:cphA protein, Aeromonas hydrophila; D015032:Zinc