Isolation and identification of stem cells from adult cashmere goat skin.
BACKGROUND
Continuously renewing epithelia are maintained by stem cells that slowly proliferate and remain in the tissues for life. It has been known for decades that mouse epithelial stem cells can be selected by adherence to specific integrins.


METHODS
The adherence of cashmere goat epidermal cells to collagen type IV for 10 min was used to obtain enriched epidermal stem cells. The characteristics of the rapidly adherent epidermal cells were determined.


RESULTS
The rapidly adherent epidermal cells exhibited the stem cell characteristics of immaturity, were quiescent, showed a high colony formation efficiency, and expressed candidate surface markers for epidermal stem cells (keratin 15, keratin 19, p63, CD34, and beta1-integrin).


CONCLUSIONS
The rapidly adherent epidermal cells represented the epidermal stem cell population.
Liu
Yingchun
Y
College of Bioengineering and College of Animal Science and Animal Medicine, Inner Mongolia Agricultural University, Huhhot, China.
Zhou
Huanmin
H
Gao
Feng
F
D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism / Q000502:physiology; D000704:Analysis of Variance; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D000954:Antigens, Surface / Q000378:metabolism*; D002448:Cell Adhesion / Q000502:physiology*; D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005434:Flow Cytometry; D006041:Goats; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D019012:Integrin beta1 / Q000378:metabolism; D053569:Keratin-15 / Q000378:metabolism; D053539:Keratin-19 / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D015345:Oligonucleotide Probes
D018952:Antigens, CD34; D000954:Antigens, Surface; D019012:Integrin beta1; D053569:Keratin-15; D053539:Keratin-19; D008565:Membrane Proteins; D015345:Oligonucleotide Probes