title: Hematopoietic contribution to skeletal muscle regeneration in acid alpha-glucosidase knockout mice.
abstract: Recent studies have shown that cells from bone marrow (BM) can give rise to differentiated skeletal muscle fibers. However, the mechanisms and identities of the cell types involved remain unknown. We performed BM transplantation in acid alpha-glucosidase (GAA) knockout mice, a model of glycogen storage disease type II, and our observations suggested that the BM cells contribute to skeletal muscle fiber formation. Furthermore, we showed that most CD45+:Sca1+ cells have a donor character in regenerating muscle of recipient mice. Based on these findings, CD45+:Sca1+ cells were sorted from regenerating muscles. The cell number was increased with granulocyte colony-stimulating factor after cardiotoxin injury, and the cells were transplanted directly into the tibialis anterior (TA) muscles of GAA knockout mice. Sections of the TA muscles stained with anti-laminin-alpha2 antibody showed that the number of CD45+:Sca1+ cells contributing to muscle fiber formation and glycogen levels were decreased in transplanted muscles. Our results indicated that hematopoietic stem cells, such as CD45+:Sca1+ cells, are involved in skeletal muscle regeneration.
authors.affiliation: Department of Pediatrics, Kyoto Prefectural University of Medicine, Graduate School of Medicine, Kamigyo-ku, Kyoto, Japan. jun1113@koto.kpu-m.ac.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Mori
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Ishihara
authors.forename: Kensuke
authors.initials: K
authors.lastname: Matsuo
authors.forename: Hisakazu
authors.initials: H
authors.lastname: Nakajima
authors.forename: Naoto
authors.initials: N
authors.lastname: Terada
authors.forename: Kitaro
authors.initials: K
authors.lastname: Kosaka
authors.forename: Zenro
authors.initials: Z
authors.lastname: Kizaki
authors.forename: Tohru
authors.initials: T
authors.lastname: Sugimoto
mesh_terms: D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D006003:Glycogen / Q000378:metabolism; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000502:physiology*; D017493:Leukocyte Common Antigens / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D018482:Muscle, Skeletal / Q000502:physiology*; D009412:Nerve Fibers / Q000502:physiology; D012038:Regeneration; D000520:alpha-Glucosidases / Q000235:genetics*
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D006003:Glycogen; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse; D000520:alpha-Glucosidases