Assessment of goat semen freezability according to the spermatozoa characteristics from fresh and frozen samples.
A total of 110 ejaculates were assessed in order to determine the influence of the physical parameters of goat semen on post-thaw motility and acrosome integrity. Sperm ejaculate characteristics, sperm motility, morphology and acrosome integrity were assessed in fresh and frozen samples by the sperm class analyzer (SCA) and Spermac staining technique. A decrease in acrosome integrity and sperm motility was found after thawing (P<0.01). Six semen parameters assessed before freezing were identified as predictors of sperm freezability (P<0.01). The percentage of morphological abnormalities (R=0.856) and motile sperm cells (R=0.655) in fresh semen are the best predictors to know the total post-thaw variability parameters.
Dorado
J
J
Department of Medicine and Animal Surgery, University of Cordoba,Spain. jdorado@uco.es
Hidalgo
M
M
Muñoz
A
A
Rodríguez
I
I
D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D006041:Goats; D006358:Hot Temperature; D008297:Male; D008565:Membrane Proteins / Q000648:ultrastructure; D012661:Semen / Q000502:physiology*; D012662:Semen Preservation / Q000009:adverse effects / Q000662:veterinary*; D013081:Sperm Motility; D013094:Spermatozoa / Q000002:abnormalities / Q000502:physiology* / Q000648:ultrastructure
C512729:ACRV1 protein, human; D008565:Membrane Proteins