title: E-cadherin expression in the subepithelial nevus cells of the giant congenital nevocellular nevi (GCNN) correlates with their migration ability in vitro.
abstract: BACKGROUND
  Giant congenital nevocellular nevi (GCNN) are histologically characterized by the broad distribution of nevus cells in the epidermis and dermis.
  
  
  OBJECTIVE
  To characterize E-cadherin in GCNN and define its role in nevic cell migrations.
  
  
  METHODS
  Twenty-four cases were immunohistochemically examined and in five cases cells were isolated for primary culture for migration assays.
  
  
  RESULTS
  The nevus cells in the superficial region showed the immunoreactivity of E-cadherin in a membranous pattern, but those in the deep part of dermis had little immunoreactivity. Ultra-structural analysis of the superficial nevus cells revealed that E-cadherin immunodeposits in the fibrillar processes around the cell body in a spotted pattern. This distribution pattern is quite different from that in the adherens junction of skin squamous epithelial cells. Boyden chamber experiments were performed using primary cultures of intradermal nevus cells. EDTA pretreatment reduced cell migration to the E-cadherin positive side when the E-cadherin positive population was relatively large in the primary cultures.
  
  
  CONCLUSIONS
  These results indicate that E-cadherin in the nevus cells may affect nevus cell motility rather than intercellular attachment.
authors.affiliation: Department of Plastic Surgery, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Matsuda
authors.forename: K
authors.initials: K
authors.lastname: Katsube
authors.forename: S
authors.initials: S
authors.lastname: Mikami
authors.forename: Y
authors.initials: Y
authors.lastname: Katsuki
authors.forename: H
authors.initials: H
authors.lastname: Iseki
authors.forename: M
authors.initials: M
authors.lastname: Mukai
authors.forename: A
authors.initials: A
authors.lastname: Yamaguchi
authors.forename: Y
authors.initials: Y
authors.lastname: Takano
authors.forename: T
authors.initials: T
authors.lastname: Nakajima
authors.forename: H
authors.initials: H
authors.lastname: Nakajima
authors.forename: K
authors.initials: K
authors.lastname: Kishi
mesh_terms: D000328:Adult; D015820:Cadherins / Q000378:metabolism*; D002465:Cell Movement; D002675:Child, Preschool; D000078404:Epidermal Cells; D004817:Epidermis / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D007223:Infant; D016253:Microscopy, Immunoelectron; D018330:Nevus, Intradermal / Q000151:congenital* / Q000378:metabolism* / Q000473:pathology; D012878:Skin Neoplasms / Q000151:congenital / Q000378:metabolism / Q000473:pathology; D051176:beta Catenin / Q000378:metabolism*
chemical_list: D015820:Cadherins; D051176:beta Catenin