title: High throughput 'omics' approaches to assess the effects of phytochemicals in human health studies.
abstract: Human health is affected by many factors. Diet and inherited genes play an important role. Food constituents, including secondary metabolites of fruits and vegetables, may interact directly with DNA via methylation and changes in expression profiles (mRNA, proteins) which results in metabolite content changes. Many studies have shown that food constituents may affect human health and the exact knowledge of genotypes and food constituent interactions with both genes and proteins may delay or prevent the onset of diseases. Many high throughput methods have been employed to get some insight into the whole process and several examples of successful research, namely in the field of genomics and transcriptomics, exist. Studies on epigenetics and RNome significance have been launched. Proteomics and metabolomics need to encompass large numbers of experiments and linked data. Due to the nature of the proteins, as well as due to the properties of various metabolites, experimental approaches require the use of comprehensive high throughput methods and a sufficiency of analysed tissue or body fluids. In this contribution, we describe the basic tools currently used in nutrigenomics studies and indicate the general requirements for future technology methodological routings.
authors.affiliation: Crop Research Institute, Drnovská 507, 161 06 Prague 6, Ruzyne, Czech Republic. ovesna@vurv.cz
authors.forename: Jaroslava
authors.initials: J
authors.lastname: Ovesná
authors.forename: Ondrej
authors.initials: O
authors.lastname: Slabý
authors.forename: Olivier
authors.initials: O
authors.lastname: Toussaint
authors.forename: Milan
authors.initials: M
authors.lastname: Kodícek
authors.forename: Petr
authors.initials: P
authors.lastname: Marsík
authors.forename: Vladimíra
authors.initials: V
authors.lastname: Pouchová
authors.forename: Tomás
authors.initials: T
authors.lastname: Vanĕk
mesh_terms: D001685:Biological Factors / Q000494:pharmacology*; D004032:Diet; D005502:Food; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D055432:Metabolomics / Q000379:methods; D054647:Nutrigenomics / Q000379:methods*; D009747:Nutritional Physiological Phenomena / Q000235:genetics*; D040901:Proteomics / Q000379:methods
chemical_list: D001685:Biological Factors