Screening method for detection of hydrocarbon-oxidizing bacteria in oil-contaminated water and soil specimens.
Toxic action of crude oil on the living world and ecosystems in general is a global problem of both aquatic and terrestrial environments. Bearing in mind the possibility of biodegradation of this toxicant, the procedures of determining counts and activity of cultivable microorganisms, and especially of bacteria responsible for degradation processes, are of great significance. The aim of this work was to study the possibility of modifying some solid media by adding triphenyltetrazolium chloride reagent as an indicator of the dehydrogenase activity, to develop a simple screening method for a simultaneous assessment of the count and activity of cultivable hydrocarbon-oxidizing bacteria in the oil-contaminated environments. The modified method appeared to be rapid and very suitable for the intended purposes.
Olga
Petrović
P
Faculty of Sciences, Department of Biology and Ecology, Trg D. Obradovica 5, 21000 Novi Sad, Serbia. petrovico@ib.ns.ac.yu
Petar
Knezević
K
Jelena
Marković
M
Srdjan
Roncević
R
D001419:Bacteria / Q000302:isolation & purification* / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D006838:Hydrocarbons / Q000378:metabolism*; D007202:Indicators and Reagents / Q000378:metabolism; D050296:Microbial Viability; D010084:Oxidation-Reduction; D012988:Soil Microbiology; D013778:Tetrazolium Salts / Q000378:metabolism; D014871:Water Microbiology
D003470:Culture Media; D006838:Hydrocarbons; D007202:Indicators and Reagents; D013778:Tetrazolium Salts; C009591:triphenyltetrazolium