Ex vivo antioxidant effects of D-004, a lipid extract from Roystonea regia fruits, on rat prostate tissue.
OBJECTIVE
To investigate whether oral treatment with D-004, a lipid extract of the Cuban royal palm fruit, produces antioxidant effects in the prostate tissue of normal and testosterone (T)-treated rats.


METHODS
In our first experiment, normal rats were distributed into five groups: one group treated with the vehicle and four groups treated with D-004 (100, 200, 400 or 800 mg/kg). In our second experiment, rats were randomized into five groups: a negative control group and four T-injected groups. The latter were comprised of a positive control group treated with the vehicle, and three groups treated with D-004 (200, 400 or 800 mg/kg).


RESULTS
In normal rats, D-004 (100-800 mg/kg) inhibited significantly and dose-dependently iron-initiated malondialdehyde (MDA) accumulation in prostate homogenates (35.7%-80.0%) vs the controls. D-004 (200-800 mg/kg) significantly reduced baseline MDA and carbonyl groups in prostate homogenates of normal rats to approximately 80% and 50%, respectively, and totally (100%) in T-treated rats.


CONCLUSIONS
Oral treatment with D-004 reduced MDA and carbonyl groups dose-dependently and markedly in normal and T-injected rats. These findings show that D-004 given at doses effective to prevent prostate hyperplasia also produces antioxidant effects in the prostate tissue.
Perez
Yohani
Y
Laboratory of Biochemistry, Center of Natural Products, National Center for Scientific Research, Havana City, Cuba. yohani.perez@cnic.edu.cu
Molina
Vivian
V
Mas
Rosa
R
Menendez
Roberto
R
Gonzalez
Rosa M
RM
Oyarzabal
Ambar
A
Jimenez
Sonia
S
D000284:Administration, Oral; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D028023:Arecaceae; D004305:Dose-Response Relationship, Drug; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000517:prevention & control; D051381:Rats; D017208:Rats, Wistar
D000975:Antioxidants; C504503:D 004 extract; D010936:Plant Extracts; D008315:Malondialdehyde