title: Relationship between exhaled nitric oxide measured by two offline methods and bronchial hyperresponsiveness in Japanese adults with asthma.
abstract: BACKGROUND
  Exhaled nitric oxide (eNO) is a useful marker of eosinophilic airway inflammation in asthmatics. There have been no studies to show the relationship between eNO measured by offline methods and the degree of bronchial hyperresponsiveness in asthmatic patients treated with inhaled corticosteroids.
  
  
  METHODS
  The study population comprised asthmatics at our outpatient clinic. We measured eNO levels by two methods ("eNOs" was measured with a Sievers kit; and "eNOc" was measured with a kit from the Center for Environmental Information Science, Japan). We also used spirometry to test bronchial hyperresponsiveness to acetylcholine (PC(20Ach)).
  
  
  RESULTS
  We recruited 192 stable asthmatics. There was a significant relationship between eNOs and eNOc (r = 0.919, p < 0.001). LogPC(20Ach) levels were negatively correlated with eNOs or eNOc levels (eNOs, r = -0.31, p < 0.001; eNOc, r = -0.23, p = 0.0013). We classified the subjects into two groups based on eNOs levels ((A) the subjects with high eNOs levels (n = 92) and (B) the subjects with normal eNOs levels (n = 100)) ; logPC(20Ach) was significantly correlated with eNOs (r = -0.34, p = 0.001) or eNOc (r = -0.28, p = 0.0075) but not correlated with %FEV(1) in (A), whereas logPC(20Ach) was not significantly correlated with eNO but significantly correlated with %FEV(1) (r = 0.33, p = 0.002) in (B).
  
  
  CONCLUSIONS
  Levels of eNOs and eNOc were correlated with the degree of bronchial hyperresponsiveness to acetylcholine in adult asthmatics treated with inhaled corticosteroids. Our findings suggest that offline monitoring of eNO will facilitate the management of bronchial asthma in patients treated with these drugs.
authors.affiliation: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital. t-tuburai@Sagamihara-hosp.gr.jp
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tsuburai
authors.forename: Naomi
authors.initials: N
authors.lastname: Tsurikisawa
authors.forename: Sonoko
authors.initials: S
authors.lastname: Morita
authors.forename: Hideki
authors.initials: H
authors.lastname: Hasunuma
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kanegae
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ishimaru
authors.forename: Yuma
authors.initials: Y
authors.lastname: Fukutomi
authors.forename: Hidenori
authors.initials: H
authors.lastname: Tanimoto
authors.forename: Emiko
authors.initials: E
authors.lastname: Ono
authors.forename: Chiyako
authors.initials: C
authors.lastname: Oshikata
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Sekiya
authors.forename: Mamoru
authors.initials: M
authors.lastname: Otomo
authors.forename: Yuji
authors.initials: Y
authors.lastname: Maeda
authors.forename: Masami
authors.initials: M
authors.lastname: Taniguchi
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Ikehara
authors.forename: Kazuo
authors.initials: K
authors.lastname: Akiyama
mesh_terms: D000280:Administration, Inhalation; D000305:Adrenal Cortex Hormones / Q000008:administration & dosage; D000328:Adult; D000368:Aged; D001249:Asthma / Q000175:diagnosis* / Q000188:drug therapy; D001944:Breath Tests / Q000379:methods; D016535:Bronchial Hyperreactivity / Q000175:diagnosis*; D001985:Bronchial Provocation Tests / Q000379:methods*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000032:analysis*; D013147:Spirometry
chemical_list: D000305:Adrenal Cortex Hormones; D009569:Nitric Oxide