title: Effect of salmon melanin-concentrating hormone and mammalian gonadotrophin-releasing hormone on somatolactin release in pituitary culture of Cichlasoma dimerus.
abstract: We detected a close morphological association between melanin-concentrating hormone (MCH)-immunoreactive (ir) fibers and somatolactin (SL)-ir cells in the pars intermedia of the cichlid fish Cichlasoma dimerus by double-label immunofluorescence. Male pituitaries obtained from adult C. dimerus were incubated with 0.1-10 microM salmon MCH, and the amount of SL released into the culture medium was semi-quantified by Western blot. This assay showed an increase of SL release in a dose-dependent manner (linear regression: P<0.05). A close association of GnRH-ir fibers with SL-ir cells was also detected at the pars intermedia level. Male pituitaries were also incubated with 0.1-10 microM of mammalian GnRH, and SL release was semi-quantified by Western blot, showing an increase of released SL levels in a dose-dependent manner (linear regression: P<0.05). In contrast, SL release was unaffected from female pituitaries incubated with salmon MCH; however, an increasing tendency was observed when mammalian GnRH was used. Hypothalamic close association of MCH-ir perikarya and GnRH-ir fibers was found by double-label immunofluorescence indicating a possible relationship between them. These results suggest that SL, like other pituitary hormones, is under hypothalamic control and is involved in diverse physiological processes including background adaptation and reproduction. This study has also shown that the in vitro culture of a single C. dimerus pituitary is a feasible method for studying the control of SL release and other pituitary hormones.
authors.affiliation: Laboratorio de Embriología Animal, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, C1428EHA, Buenos Aires, Argentina. mcanepa@bg.fcen.uba.ar
authors.forename: Maximiliano
authors.initials: M
authors.lastname: Cánepa
authors.forename: Andrea
authors.initials: A
authors.lastname: Pozzi
authors.forename: Antonio
authors.initials: A
authors.lastname: Astola
authors.forename: María Cristina
authors.initials: MC
authors.lastname: Maggese
authors.forename: Paula
authors.initials: P
authors.lastname: Vissio
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D023681:Cichlids / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D005240:Feasibility Studies; D005260:Female; D029941:Fish Proteins / Q000378:metabolism*; D007987:Gonadotropin-Releasing Hormone / Q000494:pharmacology*; D013006:Growth Hormone / Q000737:chemistry / Q000378:metabolism*; D007028:Hypothalamic Hormones / Q000494:pharmacology*; D007700:Kinetics; D008297:Male; D008543:Melanins / Q000494:pharmacology*; D008970:Molecular Weight; D010902:Pituitary Gland / Q000166:cytology; D010907:Pituitary Hormones / Q000494:pharmacology*; D020033:Protein Isoforms; D015203:Reproducibility of Results; D012474:Salmon; D013997:Time Factors
chemical_list: D003470:Culture Media; D029941:Fish Proteins; D007028:Hypothalamic Hormones; D008543:Melanins; D010907:Pituitary Hormones; D020033:Protein Isoforms; D007987:Gonadotropin-Releasing Hormone; C019463:melanin-concentrating hormone; D013006:Growth Hormone