Optimization of culture conditions for the production of thermostable polygalacturonase by Penicillium SPC-F 20.
Penicillium strain isolated from citrus fruit was found to produce thermostable polygalacturonases. Optimization of process parameters resulted in high levels of enzyme production after 3 days of incubation at a pH of 5.0 at 30 degrees C in the presence of 1% pectin. The optimum temperature for enzyme activity was 60 degrees C and a pH of 5.5 was found to be the optimal pH. The enzyme showed a high level of thermostability in the presence of substrate with a residual activity of 48% after 2 h of incubation at 60 degrees C. A thermostable nature with a high pH range for activity makes it an industrially important enzyme.
Mathew
Abraham
A
Post Graduate and Research Department of Botany, St. Peter's College, Kolenchery P.O., Kerala, 682311, India. abrahambotany@yahoo.com
Eldo
Abi N
AN
Molly
A G
AG
D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D009584:Nitrogen / Q000378:metabolism; D010368:Pectins / Q000378:metabolism; D010407:Penicillium / Q000201:enzymology*; D011096:Polygalacturonase / Q000096:biosynthesis*; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D012965:Sodium Chloride; D010368:Pectins; D011096:Polygalacturonase; D009584:Nitrogen