title: Biocompatibility of a bicarbonate-buffered amino-acid-based solution for peritoneal dialysis.
abstract: Amino-acid-based peritoneal dialysis (PD) fluids have been developed to improve the nutritional status of PD patients. As they may potentially exacerbate acidosis, an amino-acid-containing solution buffered with bicarbonate (Aminobic) has been proposed to effectively maintain acid-base balance. The aim of this study was to evaluate the mesothelial biocompatibility profile of this solution in comparison with a conventional low-glucose-based fluid. Omentum-derived human peritoneal mesothelial cells (HPMC) were preexposed to test PD solutions for up to 120 min, then allowed to recover in control medium for 24 h, and assessed for heat-shock response, viability, and basal and stimulated cytokine [interleukin (IL)-6] and prostaglandin (PGE(2)) release. Acute exposure of HPMC to conventional low-glucose-based PD solution resulted in a time-dependent increase in heat-shock protein (HSP-72) expression, impaired viability, and reduced ability to release IL-6 in response to stimulation. In contrast, in cells treated with Aminobic, the expression of HSP-72 was significantly lower, and viability and cytokine-producing capacity were preserved and did not differ from those seen in control cells. In addition, exposure to Aminobic increased basal release of IL-6 and PGE(2). These data point to a favorable biocompatibility profile of the amino-acid-based bicarbonate-buffered PD solution toward HPMC.
authors.affiliation: Department of Nephrology and Medical Intensive Care, Charité Universitätsmedizin Berlin, Campus Virchow-Klinikum, Berlin, Germany.
authors.forename: Thorsten O
authors.initials: TO
authors.lastname: Bender
authors.forename: Janusz
authors.initials: J
authors.lastname: Witowski
authors.forename: Christoph
authors.initials: C
authors.lastname: Aufricht
authors.forename: Michaela
authors.initials: M
authors.lastname: Endemann
authors.forename: Ulrich
authors.initials: U
authors.lastname: Frei
authors.forename: Jutta
authors.initials: J
authors.lastname: Passlick-Deetjen
authors.forename: Achim
authors.initials: A
authors.lastname: Jörres
mesh_terms: D000596:Amino Acids / Q000737:chemistry*; D001639:Bicarbonates / Q000737:chemistry*; D002021:Buffers; D015314:Dialysis Solutions / Q000737:chemistry*; D015232:Dinoprostone / Q000378:metabolism; D050884:HSP72 Heat-Shock Proteins / Q000096:biosynthesis; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008422:Materials Testing; D010530:Peritoneal Dialysis
chemical_list: D000596:Amino Acids; D001639:Bicarbonates; D002021:Buffers; D015314:Dialysis Solutions; D050884:HSP72 Heat-Shock Proteins; D015850:Interleukin-6; D015232:Dinoprostone