title: Lipoelectric modification of ion channel voltage gating by polyunsaturated fatty acids.
abstract: Polyunsaturated fatty acids (PUFAs) have beneficial effects on epileptic seizures and cardiac arrhythmia. We report that omega-3 and omega-6 all-cis-PUFAs affected the voltage dependence of the Shaker K channel by shifting the conductance versus voltage and the gating charge versus voltage curves in negative direction along the voltage axis. Uncharged methyl esters of the PUFAs did not affect the voltage dependence, whereas changes of pH and charge mutations on the channel surface affected the size of the shifts. This suggests an electrostatic effect on the channel's voltage sensors. Monounsaturated and saturated fatty acids, as well as trans-PUFAs did not affect the voltage dependence. This suggests that fatty acid tails with two or more cis double bonds are required to place the negative carboxylate charge of the PUFA in a position to affect the channel's voltage dependence. We propose that charged lipophilic compounds could play a role in regulating neuronal excitability by electrostatically affecting the channel's voltage sensor. We believe this provides a new approach for pharmacological treatment that is voltage sensor pharmacology.
authors.affiliation: Department of Clinical and Experimental Medicine, Division of Cell Biology, Linköping University, Linköping, Sweden.
authors.forename: Sara I
authors.initials: SI
authors.lastname: Börjesson
authors.forename: Sven
authors.initials: S
authors.lastname: Hammarström
authors.forename: Fredrik
authors.initials: F
authors.lastname: Elinder
mesh_terms: D000818:Animals; D004281:Docosahexaenoic Acids / Q000378:metabolism; D004594:Electrophysiology; D005231:Fatty Acids, Unsaturated / Q000032:analysis / Q000502:physiology*; D006863:Hydrogen-Ion Concentration; D015640:Ion Channel Gating / Q000502:physiology*; D008274:Magnesium / Q000502:physiology; D008564:Membrane Potentials; D009865:Oocytes / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051637:Shaker Superfamily of Potassium Channels / Q000502:physiology*; D014982:Xenopus laevis
chemical_list: D005231:Fatty Acids, Unsaturated; D051637:Shaker Superfamily of Potassium Channels; D004281:Docosahexaenoic Acids; D008274:Magnesium