title: Derivatized 2-furoyl-LIGRLO-amide, a versatile and selective probe for proteinase-activated receptor 2: binding and visualization.
abstract: The proteinase-activated receptor-2 (PAR2)-activating peptide with an N-terminal furoyl group modification, 2-furoyl-LIGRLO-NH2 (2fLI), was derivatized via its free ornithine amino group to yield [3H]propionyl-2fLI and Alexa Fluor 594-2fLI that were used as receptor probes for ligand binding assays and receptor visualization both for cultured cells in vitro and for colonic epithelial cells in vivo. The binding of the radiolabeled and fluorescent PAR2 probes was shown to be present in PAR2-transfected Kirsten normal rat kidney cells, but not in vector-alone-transfected cells, and was abolished by pretreatment of cells with saturating concentrations of receptor-selective PAR2 peptide agonists such as SLIGRL-NH2 and the parent agonist 2fLI but not by reverse-sequence peptides such as 2-furoyl-OLRGIL-NH2 that cannot activate PAR2. The relative orders of potencies for a series of PAR2 peptide agonists to compete for the binding of [3H]propionyl-2fLI (2fLI >> SLIGRL-NH2 approximately= trans-cinnamoyl-LIGRLO-NH2 > SLIGKV-NH2 > SLIGKT-NH2) mirrored qualitatively their relative potencies for PAR2-mediated calcium signaling in the same cells or for vasorelaxation in a rat aorta vascular assay. In the vascular assay, the potency of Alexa Fluor 594-2fLI was the same as 2fLI. We conclude that ornithine-derivatized 2fLI peptides are conveniently synthesized PAR2 probes that will be of value for future studies of receptor binding and visualization.
authors.affiliation: Department of Pharmacology and Therapeutics, University of Calgary Faculty of Medicine, 3330 Hospital Drive NW, Calgary, AB T2N4N1, Canada. mhollenb@ucalgary.ca
authors.forename: Morley D
authors.initials: MD
authors.lastname: Hollenberg
authors.forename: Bernard
authors.initials: B
authors.lastname: Renaux
authors.forename: Eric
authors.initials: E
authors.lastname: Hyun
authors.forename: Steeve
authors.initials: S
authors.lastname: Houle
authors.forename: Nathalie
authors.initials: N
authors.lastname: Vergnolle
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Saifeddine
authors.forename: Rithwik
authors.initials: R
authors.lastname: Ramachandran
mesh_terms: D000818:Animals; D001667:Binding, Competitive; D002460:Cell Line; D005456:Fluorescent Dyes / Q000737:chemistry; D008024:Ligands; D008856:Microscopy, Fluorescence; D015335:Molecular Probes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D009930:Organic Chemicals / Q000737:chemistry; D011485:Protein Binding; D011869:Radioligand Assay; D051381:Rats; D044464:Receptor, PAR-2 / Q000235:genetics / Q000378:metabolism*; D015203:Reproducibility of Results; D013329:Structure-Activity Relationship; D014162:Transfection
chemical_list: C487404:2-furoyl-LIGRLO-amide; C417664:Alexa594; D005456:Fluorescent Dyes; D008024:Ligands; D015335:Molecular Probes; D009842:Oligopeptides; D009930:Organic Chemicals; D044464:Receptor, PAR-2