The toxicity and physiological effect of goniothalamin, a styryl-pyrone, on the generalist herbivore, Spodoptera exigua Hübner.
Efficacy of Goniothalamin a styryl-pyrone isolated from Goniothalamus wightii Hook.f. and Thoms, against beet armyworm, Spodoptera exigua (Hübner), populations was determined under laboratory condition. The experiments were carried out with concentrations of 5, 10, 15 and 30 ppm in an artificial diet and compared with control insects. Laboratory bioassay showed that the goniothalamin had a strong effect on food utilization, moulting and gut histology. The food consumption and conversion of ingested and digested food to body matter decreased with increasing pyrone concentration. The antifeedant activity was also observed in larvae of S. exigua. The treated third instar larvae exhibited mortality in a dose dependent manner. At 5, 10, 15 and 30 ppm/insect, the pyrone gave 23%, 45% 63% and 100% mortality respectively. The larvae of S. exigua gained significantly less weight until pupation in the 10 and 15 ppm pyrone concentrations. Duration of larval period was also affected after treatment with pyrone. The metamorphosis was delayed with additional moulting (7th instar) after treatment with 10 and 15 ppm of goniothalamin, the percentage of larvae successfully moulted into progressive instars was significantly decreased with an increase in pyrone concentrations. The effects of goniothalamin on midgut ultrastructure of third instar larvae of S. exigua were investigated by using light microscopy. Cross sections of the midgut showed that the epithelial cells were destroyed. Significant damage of the midgut epithelium was observed along with lysis.
Senthil-Nathan
Sengottayan
S
Plant Environment Division, Honam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration, #381 Songhak-dong, Iksan, Chonbuk, Republic of Korea. senthil@ms-university.org
Choi
Man-Young
MY
Paik
Chae-Hoon
CH
Kalaivani
Kandaswamy
K
D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D006128:Growth / Q000187:drug effects; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D018978:Molting / Q000187:drug effects; D009753:Nutritive Value; D011679:Pupa; D011753:Pyrones / Q000633:toxicity*; D018411:Spodoptera / Q000502:physiology*; D015430:Weight Gain / Q000187:drug effects
D011753:Pyrones; C103280:goniothalamin