title: Delta opioid receptors mediate chemotaxis in bone marrow-derived dendritic cells.
abstract: Endogenous opioid peptides are locally produced at the inflammatory site where antigens are captured and processed by dendritic cells (DCs). Subsequently, maturing DCs migrate towards draining lymph nodes to initiate T cell response. Given the primordial role of DCs in adaptive immune response, we examined whether opioids may affect the migratory response of DCs. We found that the delta opioid receptor (DOR) mRNA was expressed at low level in bone marrow-derived immature DCs and up-regulated upon DC maturation. Moreover, DOR agonists triggered DC chemotaxis in vitro. In vivo, enkephalins prevented the egress of mature DCs injected into the peritoneal cavity of normal mice. This effect was inhibited by blocking opioid receptors on mature DCs. The cross-talk between CCR7 and DOR receptors that are both up-regulated during DC maturation was then examined. Whereas opioids did not alter the migratory responsiveness to CCR7 ligands, DOR-mediated mobilization of mature DCs was inhibited by CCL19 and CCL21 suggesting that the opioid chemotactic activity decreases as the concentration of the chemokines increases.
authors.affiliation: INSERM, U563, Centre de Physiopathologie de Toulouse Purpan, Toulouse, France.
authors.forename: Alan
authors.initials: A
authors.lastname: Bénard
authors.forename: Jérôme
authors.initials: J
authors.lastname: Boué
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Chapey
authors.forename: Martial
authors.initials: M
authors.lastname: Jaume
authors.forename: Bruno
authors.initials: B
authors.lastname: Gomes
authors.forename: Gilles
authors.initials: G
authors.lastname: Dietrich
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D002478:Cells, Cultured; D054415:Chemokine CCL19 / Q000502:physiology; D054421:Chemokine CCL21 / Q000502:physiology; D002634:Chemotaxis, Leukocyte / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism* / Q000637:transplantation; D020881:Enkephalin, D-Penicillamine (2,5)- / Q000819:agonists / Q000096:biosynthesis / Q000502:physiology; D018836:Inflammation Mediators / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D020239:Receptor Cross-Talk / Q000276:immunology; D054400:Receptors, CCR7 / Q000502:physiology; D017465:Receptors, Opioid, delta / Q000819:agonists / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000502:physiology*; D015854:Up-Regulation / Q000276:immunology
chemical_list: C515461:Ccl19 protein, mouse; C515371:Ccr7 protein, mouse; D054415:Chemokine CCL19; D054421:Chemokine CCL21; D018836:Inflammation Mediators; D054400:Receptors, CCR7; D017465:Receptors, Opioid, delta; D020881:Enkephalin, D-Penicillamine (2,5)-