Evaluation of an immunodot test to manage white spot syndrome virus (WSSV) during cultivation of the giant tiger shrimp Penaeus monodon.
A monoclonal antibody-based immunodot test was compared to a polymerase chain reaction (PCR) assay for managing white spot syndrome virus (WSSV) on shrimp farms at Kundapur and Kumta situated in Udupi and Uttar Kannada Districts, respectively, of Karnataka on the west coast of India. Of 12 grow-out farms in Kundapur, 6 (F1 to F6) yielded shrimp samples that were negative for WSSV by both immunodot test and 1-step PCR from stocking to successful harvest. Samples from the other 6 farms (F7 to F12) were positive for WSSV by both immunodot test and 1-step PCR at various times post stocking, and their crops failed. In the 2 farms at Kumta (F13, F14), immunodot and 1-step PCR results were both negative, and harvests were successful. In contrast to 1-step PCR results, farms F5, F6, F13, and F14 gave positive results for WSSV by 2-step PCR, and they were successfully harvested at 105 d post stocking. Our results indicate that an inexpensive immunodot assay can be used to replace the more expensive 1-step PCR assay for disease monitoring.
Patil
Rajreddy
R
Fish Pathology and Biotechnology Laboratory, Department of Aquaculture, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore 575 002, India.
Palaksha
K J
KJ
Anil
T M
TM
Guruchannabasavanna
Patil
Prakash
P
Shankarl
K M
KM
Mohan
C V
CV
Sripada
A
A
D000818:Animals; D017756:Aquaculture; D005663:Furans; D005880:Gills / Q000821:virology; D015151:Immunoblotting / Q000191:economics / Q000379:methods*; D007194:India; D033561:Penaeidae / Q000821:virology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013876:Thiophenes; D046848:White spot syndrome virus 1 / Q000276:immunology* / Q000302:isolation & purification* / Q000502:physiology
C516387:3,4-bis-(2,4,5-trimethyl-thiophen-3-yl)furan-2,5-dione; D005663:Furans; D013876:Thiophenes