[The genetic diversity of Cymbidium by ISSR].
ISSR was applied to detect the relationship between 16 Cymbidium species, and 836 bands were amplified with 15 primers, including 227 polymorphic bands. The polymorphic percentage is 27.2%. UPGMA results showed that the genetic distance were closest between C.goeringii (Rchb.f.) Rchb.f. and C. goeringii var. longibracteatum, and C.lancifolium Hook. was far away from the other 15 species. This result is quite similar to the traditional classification, indicating that the technique could supplement some information to traditional taxonomy in the molecular level.
Wu
Zhen-Xing
ZX
Key Laboratory of Biochemistry and Molecular Biology, Hangzhou Normal University, Hangzhou 310018,China. wuzhenxing007@126.com
Wang
Hui-Zhong
HZ
Shi
Nong-Nong
NN
Zhao
Yan
Y
D018744:DNA, Plant / Q000235:genetics; D014644:Genetic Variation / Q000235:genetics*; D029595:Orchidaceae / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*
D018744:DNA, Plant