title: Lack of involvement of lipocortin 1 in dexamethasone suppression of IL-1 release.
abstract: The annexin lipocortin 1 is reported to mediate some anti-inflammatory effects of glucocorticoids, but the mechanisms of this mediation are incompletely understood. The involvement of lipocortin 1 in glucocorticoid inhibition of monocyte interleukin 1beta (IL-1beta) release has been investigated. Treatment of peripheral blood monocytes with 2 mug/ml lipopolysaccharide potently increased IL-1beta release (p = 0.001) and dexamethasone (10(-7) M) significantly reduced both resting and stimulated IL-1beta release (p = 0.009). A neutralizing monoclonal antibody to lipocortin 1 (0.5-50.0 mug/ml) was unable to inhibit this effect and recombinant lipocortin 1 (2 x 10(-6) M) and 188aa lipocortin 1 fragment (10(-8)-10(-6) M) had no effect. It is concluded that lipocortin 1 is not involved in the inhibition of monocyte IL-1beta release by glucocorticoids.
authors.affiliation: Rheumatology Unit Monash Medical Centre Locked Bag No. 29 Clayton 3168 Australia.
authors.forename: E F
authors.initials: EF
authors.lastname: Morand
authors.forename: D
authors.initials: D
authors.lastname: Rickard
authors.forename: N J
authors.initials: NJ
authors.lastname: Goulding