title: Efficient trans-encapsidation of hepatitis C virus RNAs into infectious virus-like particles.
abstract: Recently, complete replication of hepatitis C virus (HCV) in tissue culture was established using the JFH1 isolate. To analyze determinants of HCV genome packaging and virion assembly, we developed a system that supports particle production based on trans-packaging of subgenomic viral RNAs. Using JFH1 helper viruses, we show that subgenomic JFH1 replicons lacking the entire core to NS2 coding region are efficiently encapsidated into infectious virus-like particles. Similarly, chimeric helper viruses with heterologous structural proteins trans-package subgenomic JFH1 replicons. Like authentic cell culture-produced HCV (HCVcc) particles, these trans-complemented HCV particles (HCV(TCP)) penetrate target cells in a CD81 receptor-dependent fashion. Since HCV(TCP) production was limited by competition between the helper and subgenomic RNA and to avoid contamination of HCV(TCP) stocks with helper viruses, we created HCV packaging cells. These cells encapsidate various HCV replicons with high efficiency, reaching infectivity titers up to 10(6) tissue culture infectious doses 50 per milliliter. The produced particles display a buoyant density comparable to HCVcc particles and can be propagated in the packaging cell line but support only a single-round infection in naïve cells. Together, this work demonstrates that subgenomic HCV replicons are assembly competent, thus excluding cis-acting RNA elements in the core-to-NS2 genomic region essential for RNA packaging. The experimental system described here should be helpful to decipher the mechanisms of HCV assembly and to identify RNA elements and viral proteins involved in particle formation. Similar to other vector systems of plus-strand RNA viruses, HCV(TCP) may prove valuable for gene delivery or vaccination approaches.
authors.affiliation: Department of Molecular Virology, University of Heidelberg, Im Neuenheimer Feld 345, 69120 Heidelberg, Germany.
authors.forename: Eike
authors.initials: E
authors.lastname: Steinmann
authors.forename: Christiane
authors.initials: C
authors.lastname: Brohm
authors.forename: Stephanie
authors.initials: S
authors.lastname: Kallis
authors.forename: Ralf
authors.initials: R
authors.lastname: Bartenschlager
authors.forename: Thomas
authors.initials: T
authors.lastname: Pietschmann
mesh_terms: D015703:Antigens, CD / Q000378:metabolism; D002460:Cell Line; D005816:Genetic Complementation Test; D006378:Helper Viruses; D016174:Hepacivirus / Q000235:genetics / Q000502:physiology*; D006801:Humans; D012367:RNA, Viral / Q000378:metabolism*; D060225:Tetraspanin 28; D014771:Virion / Q000235:genetics / Q000378:metabolism; D019065:Virus Assembly / Q000235:genetics / Q000502:physiology*
chemical_list: D015703:Antigens, CD; C554798:CD81 protein, human; D012367:RNA, Viral; D060225:Tetraspanin 28