Overcoming the resistance of codling moth against conventional Cydia pomonella granulovirus (CpGV-M) by a new isolate CpGV-I12.
Recently, codling moth (CM, Cydia pomonella L.) populations with a significantly reduced susceptibility to C. pomonella granulovirus (CpGV) products have been observed in Germany. A novel CpGV isolate, designated CpGV-I12, is able to overcome the CpGV resistance. CpGV-I12 originated from Iran and showed superior efficacy in laboratory bioassays against a resistant CM strain (CpR), which has a 100-fold reduced susceptibility to commercially used isolate CpGV-M. Determination of the median lethal concentration (LC(50)) indicated that CpGV-I12 is nearly as efficient in resistant CpR as CpGV-M in a susceptible CM strain (CpS). Beyond, CpGV-I12 caused superior mortality in CpS. Infection experiments showed that the resistance breaking effect can be observed in all instars of CpR. CpGV-I12 is a promising alternative control agent of CM in orchards where conventional CpGV products fail. In addition, we demonstrate in bioassays with recombinant expressed Cry1Ab that cross-resistance to CpGV and Bacillus thuringiensis products is not likely.
Eberle
K E
KE
Laboratory of Biotechnological Crop Protection, Department of Phytopathology, Agricultural Service Center Palatinate (DLR Rheinpfalz), Breitenweg 71, 67435 Neustadt a.d. Weinstr., Germany.
Asser-Kaiser
S
S
Sayed
S M
SM
Nguyen
H T
HT
Jehle
J A
JA
D000818:Animals; D003429:Cross Reactions; D004279:DNA, Viral / Q000032:analysis; D004306:Dose-Response Relationship, Immunologic; D020022:Genetic Predisposition to Disease; D025722:Granulovirus / Q000235:genetics* / Q000472:pathogenicity; D054884:Host-Pathogen Interactions; D007113:Immunity, Innate / Q000235:genetics*; D020128:Inhibitory Concentration 50; D007305:Insecticide Resistance / Q000235:genetics*; D007814:Larva / Q000235:genetics / Q000276:immunology / Q000821:virology; D008136:Longevity / Q000276:immunology; D009036:Moths / Q000235:genetics / Q000276:immunology* / Q000821:virology; D010572:Pest Control, Biological; D015183:Restriction Mapping
D004279:DNA, Viral