Whole cell environmental biosensor on diamond.
A whole-cell environmental biosensor was fabricated on a diamond electrode. Unicellular microalgae Chlorella vulgaris was entrapped in the bovine serum albumin (BSA) membrane and immobilized directly onto the surface of a diamond electrode for heavy metal detection. We found that the unique surface properties of diamond reduce the electrode fouling problem commonly encountered with metal electrodes. The cell-based diamond biosensor can attain a detection limit of 0.1 ppb for Zn(2+) and Cd(2+), and exhibits higher detection sensitivity and stability compared to platinum electrodes.
Chong
Kwok Feng
KF
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore. chmlohkp@nus.edu.sg
Loh
Kian Ping
KP
Ang
Kailian
K
Ting
Yen Peng
YP
D015374:Biosensing Techniques; D002104:Cadmium / Q000032:analysis; D048408:Chlorella vulgaris; D018130:Diamond; D004566:Electrodes; D004785:Environmental Pollutants / Q000032:analysis*; D019216:Metals, Heavy / Q000032:analysis*; D015032:Zinc / Q000032:analysis
D004785:Environmental Pollutants; D019216:Metals, Heavy; D002104:Cadmium; D018130:Diamond; D015032:Zinc