Phytase from antarctic yeast strain Cryptococcus laurentii AL27.
Cryptococcus laurentii strain AL27 demonstrating significant potential for intracellular phytase production was selected by 2-step screening of Antarctic yeasts. The strain showed increased phytase activity in a culture medium with 40 g/L sucrose, KH2PO4 providing 5 mg/L phosphorus, and cultivation temperature of 24 degrees C, which relates it to psychrotrophic microorganisms. The enzyme kinetic characteristics according to sodium phytate were Km = 0.98 mmol/L, vlim = 33.3 micromol g(-1) min(-1). The enzyme had maximum activity at 40 degrees C and acted within a wide pH range: from 2.0 to 5.5, which is of positive significance for its direct inclusion into the feed of monogastric animals.
Pavlova
K
K
Laboratory of Applied Microbiology, Institute of Microbiology, Bulgarian Academy of Science, 4002, Plovdiv, Bulgaria. konpavlova@yahoo.com
Gargova
S
S
Hristozova
T
T
Tankova
Z
Z
D010832:6-Phytase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D000864:Antarctic Regions; D003454:Cryptococcus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007700:Kinetics; D012988:Soil Microbiology; D013696:Temperature
D005656:Fungal Proteins; D010832:6-Phytase