Detection of circulating trophoblast particles in peripheral maternal blood in preeclampsia complicated pregnancies.
OBJECTIVE
Data on trophoblast particles in peripheral maternal circulation are rare. Applying a new enrichment technique, Oncoquick, we evaluated the number of trophoblast particles in maternal blood in preeclamptic and non-preeclamptic pregnancies.


METHODS
After isolation of trophoblast particles using Oncoquick, trophoblast particles were detected by immunocytochemistry, applying a monoclonal pan cytokeratin (CK) antibody. Fetal origin of the detected particles was verified by XY- fish analysis in women delivering male infants.


RESULTS
Trophoblast particles were identified in 25/52 (48%) of preeclamptic and 1/20 (5%) of non-preeclamptic pregnancies.


CONCLUSIONS
The number of trophoblast particles in peripheral maternal blood of preeclamptic pregnancies was significantly higher than in non-preeclamptic pregnancies.
Schmidt
Markus
M
Department of Gynecology and Obstetrics, University of Duisburg-Essen, Essen, Germany. markus.schmidt@uk-essen.de
Hoffmann
Barbara
B
Beelen
Dietrich
D
Gellhaus
Alexandra
A
Winterhager
Elke
E
Kimmig
Rainer
R
Kasimir-Bauer
Sabine
S
D000328:Adult; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D007231:Infant, Newborn; D008297:Male; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis*; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology*