Identification and molecular characterization of two hepcidin genes from black rockfish (Sebastes schlegelii).
Antimicrobial peptides (AMPs) are important mediators of the immune response against bacteria and cysteine-rich peptide hepcidin is a 20-26 residues member with functions in iron regulation and antimicrobial activity. Here, we have identified two different types of hepcidin cDNA from the Black rockfish, Sebastes schlegelii, by EST analysis. Both hepcidin genes (hepcidin I and II) consist of two introns and three exons that encode a prepropeptide (88 amino acids). A TATA box and several consensus-binding motifs for transcription factors were found in the upstream of the transcriptional start site. Semi-quantitative RT-PCR analysis suggested that hepcidin I transcripts were detected in various tissues, while hepcidin II was only expressed in the liver. During the bacterial challenge with the fish pathogen, Streptococcus iniae, two hepcidin genes were differentially expressed. Hepcidin I and II dramatically increased at 24 h post-injection, then gradually declined at 3 days in hepcidin II, while hepcidin I expression continued at 3 days after challenge.
Kim
Young-Ok
YO
National Fisheries Research and Development Institute, Biotechnology Research Institute, 408-1 Sirang-ri, Gijang-up, Gijang-gun, Busan, Republic of Korea. yobest@gmail.com
Park
Eun-Mi
EM
Nam
Bo-Hye
BH
Kong
Hee Jeong
HJ
Kim
Woo-Jin
WJ
Lee
Sang-Jun
SJ
D000595:Amino Acid Sequence; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D018076:DNA, Complementary / Q000302:isolation & purification; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D023061:Gene Order; D064451:Hepcidins; D008969:Molecular Sequence Data; D010473:Perciformes / Q000235:genetics* / Q000382:microbiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D014157:Transcription Factors / Q000378:metabolism
D023181:Antimicrobial Cationic Peptides; D018076:DNA, Complementary; D029941:Fish Proteins; D064451:Hepcidins; D014157:Transcription Factors