Skin prick test of Kudoa sp. antigens in patients with gastrointestinal and/or allergic symptoms related to fish ingestion.
A majority of Kudoa spp. infects the somatic muscle of fish establishing cysts. Previously, elevated humoral responses were detected in BALB/c mice immunised with Kudoa sp. pseudocyst extracts and in BALB/c mice orally inoculated with Kudoa sp. pseudocysts, as well as the presence of anti-Kudoa sp. antibodies in human sera by enzyme-linked immunosorbent assay. The objective of this work was to test Kudoa sp. pseudocyst extracts by the skin prick test. Fifteen patients with gastroallergic and/or allergic symptoms related to fish ingestion were examined. Kudoa sp. pseudocyst extracts were administered (1 mg/ml) on the volar forearm skin. Four of the 15 selected patients were positive to Kudoa sp. extracts. The saline solution negative control did not induce any reaction.
Martínez de Velasco
Gonzalo
G
Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Rodero
Marta
M
Cuéllar
Carmen
C
Chivato
Tomás
T
Mateos
José María
JM
Laguna
Rafael
R
D000818:Animals; D000941:Antigens / Q000276:immunology*; D003063:Cnidaria / Q000276:immunology*; D005512:Food Hypersensitivity / Q000175:diagnosis*; D005542:Forearm; D005759:Gastroenteritis / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis*; D012882:Skin Tests / Q000379:methods*
D000941:Antigens