title: Histopathological and ultrastructural features of enlarged cells of humpback grouper Cromileptes altivelis challenged with Megalocytivirus (family Iridoviridae) after vaccination.
abstract: The genus Megalocytivirus in the family Iridoviridae encompasses isolates of red sea bream iridovirus (RSIV) and grouper sleepy disease iridovirus (GSDIV). In the present study, humpback grouper Cromileptes altivelis juveniles were challenged with GSDIV after vaccination with a commercial RSIV vaccine. The unvaccinated group (in duplicate) showed higher mortalities (59.3 to 66.7%) than the vaccination group (0% mortalitiy, in duplicate). Surviving fish in the vaccinated group displayed masses of enlarged cells in the spleen. Electron microscopy revealed that they contained hemosiderin granules within the cytoplasm. In contrast, moribund fish from the unvaccinated group exhibited large numbers of inclusion body-bearing cells (IBCs) in the spleen, while surviving fish displayed masses of enlarged cells in which a small number of GSDIV virions were assembled.
authors.affiliation: Graduate school of Bioresources, Mie University, Tsu, Mie 514-8507, Japan.
authors.forename: Ketut
authors.initials: K
authors.lastname: Mahardika
authors.lastname: Haryanti
authors.forename: Ahmad
authors.initials: A
authors.lastname: Muzaki
authors.forename: Teruo
authors.initials: T
authors.lastname: Miyazaki
mesh_terms: D000818:Animals; D004266:DNA Virus Infections / Q000401:mortality / Q000473:pathology / Q000517:prevention & control / Q000662:veterinary*; D005393:Fish Diseases / Q000276:immunology / Q000473:pathology* / Q000517:prevention & control* / Q000821:virology; D007497:Iridoviridae / Q000276:immunology; D007668:Kidney / Q000473:pathology; D010473:Perciformes / Q000821:virology*; D013154:Spleen / Q000473:pathology; D014611:Vaccination / Q000662:veterinary*; D015164:Vaccines, Inactivated / Q000276:immunology; D014765:Viral Vaccines / Q000276:immunology*
chemical_list: D015164:Vaccines, Inactivated; D014765:Viral Vaccines