Capture of cationic ligands bound diffusely to base pairs during DNA refolding.
We obtained the intrinsic binding affinity for metal ions, polyamines, and oligolysine peptides diffusely bound to base-paired sites in DNA by monitoring the shift of the hairpin-duplex equilibrium of the self-complementary DNA sequences, which can be widely used for capturing cationic ligands bound diffusely to nucleotide base pairs.
Nakano
Shu-ichi
S
Frontier Institute for Biomolecular Engineering Research, Konan University, 8-9-1 Okamoto, Higashinada-ku, Kobe 658-8501, Japan.
Kirihata
Toshimasa
T
Sugimoto
Naoki
N
D020029:Base Pairing; D001483:Base Sequence; D002412:Cations / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D008024:Ligands; D008239:Lysine / Q000737:chemistry; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010455:Peptides / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry
D002412:Cations; D008024:Ligands; D010455:Peptides; D011073:Polyamines; D004247:DNA; D008239:Lysine