HIV-1 protease function and structure studies with the simplicial neighborhood analysis of protein packing method.
The Simplicial Neighborhood Analysis of Protein Packing (SNAPP) method was used to predict the effect of mutagenesis on the enzymatic activity of the HIV-1 protease (HIVP). SNAPP relies on a four-body statistical scoring function derived from the analysis of spatially nearest neighbor residue compositional preferences in a diverse and representative subset of protein structures from the Protein Data Bank. The method was applied to the analysis of HIVP mutants with residue substitutions in the hydrophobic core as well as at the interface between the two protease monomers. Both wild-type and tethered structures were employed in the calculations. We obtained a strong correlation, with R(2) as high as 0.96, between DeltaSNAPP score (i.e., the difference in SNAPP scores between wild-type and mutant proteins) and the protease catalytic activity for tethered structures. However, a weaker but significant correlation was obtained for nontethered structures. Our analysis identified residues both in the hydrophobic core and at the dimeric interface that are very important for the protease function. This study demonstrates a potential utility of the SNAPP method for rational design of mutagenesis studies and protein engineering.
Zhang
Shuxing
S
Department of Experimental Therapeutics, M. D. Anderson Cancer Center, Unit 36, Houston, Texas 77030, USA.
Kaplan
Andrew H
AH
Tropsha
Alexander
A
D002384:Catalysis; D019295:Computational Biology / Q000379:methods*; D004252:DNA Mutational Analysis; D019281:Dimerization; D016333:HIV Protease / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary; D021122:Protein Subunits / Q000737:chemistry; D013329:Structure-Activity Relationship
D021122:Protein Subunits; D016333:HIV Protease; C514803:p16 protease, Human immunodeficiency virus 1