Nano-biopower supplies for biomolecular motors: the use of metabolic pathway-based fuel generating systems in microfluidic devices.
We report fuel generation systems for molecular motors based on pyruvate kinase, or for the first time, mitochondria, implemented within microfluidic devices. Intact organelles acted as bio-nanopower supplies for molecular motors, using isolated mitochondria to convert chemical energy from succinate to ATP, harnessing nature's enzymatic transformation cascades directly. Motors were activated essentially equally by ATP produced by pyruvate kinase, mitochondria, or direct addition of ATP.
Wasylycia
Joshua R
JR
Deptartment of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada.
Sapelnikova
Svetlana
S
Jeong
Hyuk
H
Dragoljic
Jelena
J
Marcus
Sandra L
SL
Harrison
D Jed
DJ
D000255:Adenosine Triphosphate / Q000737:chemistry / Q000378:metabolism*; D004736:Energy-Generating Resources; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D008928:Mitochondria / Q000201:enzymology*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D011770:Pyruvate Kinase / Q000737:chemistry / Q000378:metabolism*; D019802:Succinic Acid / Q000737:chemistry / Q000378:metabolism*
D000255:Adenosine Triphosphate; D019802:Succinic Acid; D011770:Pyruvate Kinase