[CDR3 spectratyping and clonal expansion of peripheral blood T cells in a patient with beta-mediterranean anaemia following peripheral blood stem cell transplantation].
OBJECTIVE
To study the CDR3 spectratyping and clonal expansion of T cells in the peripheral blood mononuclear cells (PBMCs) of patients with beta-mediterranean anemia patient undergoing allogeneic peripheral blood stem cell (PBSC) transplantation.


METHODS
The total RNA was isolated from the PBMCs of a nine-year-old boy with beta-mediterranean anemia before and after PBSC transplantation, and at the time of occurrence of graft-versus-host disease (GVHD). The CDR3 length was analyzed using immunoscope technique, and the characteristics of the T cell receptor (TCR) on the T cells with clonal expansion were examined by gene sequencing.


RESULTS
The 24 TCR BV CDR3 repertoire showed Gaussian distribution in the PBMCs isolated before the transplantation, and some of the TCR BV family CDR3 showed skewing in the PBMCs isolated 23 days after transplantation and at the onset of GVHD (28 days after transplantation), suggesting the clonal expansion of the donor PBSCs.


CONCLUSIONS
The host PBMCs show muti-clonal expansion 23 days after PBSC transplantation, and in the stage of GVHD, some of the TCR BV family T cells show significant monoclonal expansion. Analysis of TCR CDR3 spectratyping and the molecular characteristics of specific TCR may help evaluate the immune reconstitution following the transplantation and indicate specific treatment for potential GVHD.
Yao
Xin-sheng
XS
Institute of Molecular Immunology, School of Biotechnology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Zhou
Ming-qian
MQ
Ma
Li
L
Luo
Wei
W
Feng
Xiao-qin
XQ
Wen
Qian
Q
Wang
Xiao-ning
XN
D002648:Child; D022801:Complementarity Determining Regions / Q000235:genetics / Q000276:immunology*; D006086:Graft vs Host Disease / Q000209:etiology / Q000276:immunology*; D006801:Humans; D008297:Male; D036102:Peripheral Blood Stem Cell Transplantation / Q000009:adverse effects / Q000379:methods*; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics / Q000276:immunology*; D017086:beta-Thalassemia / Q000276:immunology* / Q000601:surgery
D022801:Complementarity Determining Regions; D016693:Receptors, Antigen, T-Cell, alpha-beta