title: [Monitoring of antibiotic resistance of gram negative anaerobes].
abstract: METHODS
  Using an agar reference method (Norma M11-A5, National Committee for Clinical and Laboratory Standards) the minimal inhibitory concentrations of nine antibiotics were determined for 376 anaerobic strains. The following strains were investigated: 254 Bacteroides fragilis group (including 143 B. fragilis), 122 other gram-negative anaerobes (Bacteroides spp., Prevotella, Fusobacterium, Porphyromonas, Suterella, Desulfomonas, Veillonella).
  
  
  RESULTS
  In the B. fragilis group resistance rates were: coamoxyclav 2.8%, ticarcillin 27.5%, ticarcillin-clavulanic acid 1.9%, piperacillin-tazobactam 1.9%, cefoxitin 6.2%, imipenem 0.8%, clindamycin 28.3%, respectively. Based on previous studies, resistance to imipenem remained low in 2003 and was only observed for B. fragilis. Resistance to clindamycin was maintained around 25%. No metronidazole resistance was observed, but decreased susceptibility was found for B. fragilis, B. merdae and Prevotella, as in 4.3% of gram-negative anaerobes.
  
  
  CONCLUSIONS
  This study confirms the high resistance rate of gram-negative anaerobes to clindamycin, the efficient activity of imipenem, beta-lactam/beta-lactamase inhibitor combinations and metronidazole. However, reduced metronidazole susceptibility seems to be increasing.
authors.affiliation: Laboratoire de bactériologie clinique, faculté des sciences pharmaceutiques et biologiques, 3, rue du Professeur-Laguesse, BP 83, 59006 Lille cedex, France.
authors.forename: E
authors.initials: E
authors.lastname: Singer
authors.forename: L
authors.initials: L
authors.lastname: Calvet
authors.forename: F
authors.initials: F
authors.lastname: Mory
authors.forename: C
authors.initials: C
authors.lastname: Muller
authors.forename: M
authors.initials: M
authors.lastname: Chomarat
authors.forename: M-C
authors.initials: MC
authors.lastname: Bézian
authors.forename: S
authors.initials: S
authors.lastname: Bland
authors.forename: M-E
authors.initials: ME
authors.lastname: Juvenin
authors.forename: H
authors.initials: H
authors.lastname: Drugeon
authors.forename: T
authors.initials: T
authors.lastname: Fosse
authors.forename: F
authors.initials: F
authors.lastname: Goldstein
authors.forename: B
authors.initials: B
authors.lastname: Jaulhac
authors.forename: H
authors.initials: H
authors.lastname: Monteil
authors.forename: H
authors.initials: H
authors.lastname: Marchandin
authors.forename: H
authors.initials: H
authors.lastname: Jean-Pierre
authors.forename: L
authors.initials: L
authors.lastname: Dubreuil
mesh_terms: D000005:Abdomen / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000145:classification / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004352:Drug Resistance, Microbial / Q000502:physiology*; D016946:Gram-Negative Aerobic Rods and Cocci / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D012867:Skin / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents