title: Hormone-sensitive lipase is necessary for normal mobilization of lipids during submaximal exercise.
abstract: For the working muscle there are a number of fuels available for oxidative metabolism, including glycogen, glucose, and nonesterified fatty acids. Nonesterified fatty acids originate from lipolysis in white adipose tissue, hydrolysis of VLDL triglycerides, or hydrolysis of intramyocellular triglyceride stores. A key enzyme in the mobilization of fatty acids from intracellular lipid stores is hormone-sensitive lipase (HSL). The aim of the present study was to investigate the metabolic response of HSL-null mice challenged with exercise or fasting and to examine whether other lipases are able to fully compensate for the lack of HSL. The results showed that HSL-null mice have reduced capacity to perform aerobic exercise. The liver glycogen stores were more rapidly depleted in HSL-null mice during treadmill exercise, and HSL-null mice had reduced plasma concentrations of both glycerol and nonesterified fatty acids after exercise and fasting, respectively. The data support the hypothesis that in the absence of HSL, mice are not able to respond to an exercise challenge with increased mobilization of the lipid stores. Consequently, the impact of the lipid-sparing effect on liver glycogen is reduced in the HSL-null mice, resulting in faster depletion of this energy source, contributing to the decreased endurance during submaximal exercise.
authors.affiliation: Department of Experimental Medical Science, Lund University, BMC C11, SE-221 84 Lund, Sweden.
authors.forename: Céline
authors.initials: C
authors.lastname: Fernandez
authors.forename: Ola
authors.initials: O
authors.lastname: Hansson
authors.forename: Pernilla
authors.initials: P
authors.lastname: Nevsten
authors.forename: Cecilia
authors.initials: C
authors.lastname: Holm
authors.forename: Cecilia
authors.initials: C
authors.lastname: Klint
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004837:Epinephrine / Q000097:blood; D005230:Fatty Acids, Nonesterified / Q000097:blood; D005990:Glycerol / Q000097:blood; D019344:Lactic Acid / Q000097:blood; D050356:Lipid Metabolism / Q000502:physiology*; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D046529:Microscopy, Electron, Transmission; D018482:Muscle, Skeletal / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D010805:Physical Conditioning, Animal; D018709:Statistics, Nonparametric; D002787:Sterol Esterase / Q000378:metabolism*; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D005230:Fatty Acids, Nonesterified; D014280:Triglycerides; D019344:Lactic Acid; D002787:Sterol Esterase; D005990:Glycerol; D004837:Epinephrine