Use of attenuated total reflectance Fourier transform infrared spectroscopy to identify microbial metabolic products on carbonate mineral surfaces.
This paper demonstrates the use of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to detect microbial metabolic products on carbonate mineral surfaces. By creating an ATR-FTIR spectral database for specific organic acids using ATR-FTIR spectroscopy we were able to distinguish metabolic acids on calcite surfaces following Escherichia coli growth. The production of these acids by E. coli was verified using high-performance liquid chromatography with refractive index detection. The development of this technique has allowed us to identify microbial metabolic products on carbonate surfaces in nutrient-limited cave environments.
Bullen
Heather A
HA
Department of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USA.
Oehrle
Stuart A
SA
Bennett
Ariel F
AF
Taylor
Nicholas M
NM
Barton
Hazel A
HA
D019342:Acetic Acid / Q000378:metabolism*; D002119:Calcium Carbonate / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004926:Escherichia coli / Q000378:metabolism*; D019289:Pyruvic Acid / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
D019289:Pyruvic Acid; D002119:Calcium Carbonate; D019342:Acetic Acid