The effect of starvation stress on the protein profiles in Flexibacter chinensis.
Analysis of many proteins produced during the transition into the stationary phase and under stress conditions (including starvation stress) demonstrated that a number of novel proteins were induced in common to each stress and could be the reason for cross-protection in bacterial cells. It is necessary to investigate the synthesis of these proteins during different stress conditions.



The changes in protein profile of Flexibacter chinensis at various stages of the starvation process and the other stresses were investigated using two-dimensional gel electrophoresis which has proven to be a powerful tool for investigation of the changes in protein profiles under such conditions.



Most starvation proteins were synthesized during the early stationary phase and many of these proteins remained during long-term starvation. Some of these proteins were transiently synthesized. The sequencing result of one of the proteins showed that there was a 62.5% identity in 8 amino acids overlapped with the 5' residue of a 10 kDa chaperon protein which is known to be involved in the starvation stress response in other organisms.



There are many proteins synthesized in common with many stresses in Flexibacter chinensis. Some of these proteins must play a major role in the stability of the cell under different stresses.
Raheb
Jamshid
J
National Institute for Genetic Engineering and Biotechnology (NIGEB), Pazhoohesh Boulvard, Kilometers 17, P.O. Box: 1455-6343, Tehran, Iran. jam@nrcgeb.ac.ir
Naghdi
Shamim
S
National Institute for Genetic Engineering and Biotechnology (NIGEB), Pazhoohesh Boulvard, Kilometers 17, P.O. Box: 1455-6343, Tehran, Iran. jam@nrcgeb.ac.ir
Flint
Ken P
KP
Dept. of Biological Sciences University of Warwick, Coventry CV4 7AL, UK.
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D001729:Bismuth; D002244:Carbon / Q000172:deficiency*; D015180:Electrophoresis, Gel, Two-Dimensional; D004926:Escherichia coli / Q000254:growth & development / Q000378:metabolism; D045248:Flexibacter / Q000254:growth & development / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology; D008969:Molecular Sequence Data; D009584:Nitrogen / Q000172:deficiency*; D009942:Organometallic Compounds; D018384:Oxidative Stress / Q000502:physiology*; D010710:Phosphates / Q000172:deficiency*; D012459:Salicylates; D013696:Temperature; D013997:Time Factors
D001426:Bacterial Proteins; D009942:Organometallic Compounds; D010710:Phosphates; D012459:Salicylates; C015715:bismuth subsalicylate; D002244:Carbon; D009584:Nitrogen; D001729:Bismuth