[Isolation and characterization of the first strain of Helicobacter hepaticus in China].
Helicobacter hepaticus is nongastric helicobacter that can reside in the hepatobiliary and intestinal systems of many animal hosts, leading to proliferative hepatitis, hepatocellular carcinoma, typhlitis, and colonitis. In this study, the intestinal mucosa was isolated from BALB/c mice to prepare tissue homogenate and spread onto selective C jejuni blood agar plates for incubation in the presence of trimethoprim, vancomycin, and polymyxin at 37 degrees Celsius; under microaerobic conditions in vented jars containing 5% O2, 10%CO2, and 85% N2. The bacteria were identified morphologically and biochemically. Gene sequence analysis of the 16s rRNA confirmed the presence of Helicobacter hepaticus, and the success in isolating this bacteria may have significant implications for studies of nongastric helicobacter.
Li
Jing
J
Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Lijing1801@126.com
Lin
Huan-jian
HJ
Jiang
Bo
B
Bai
Yang
Y
Zhang
Di
D
Zhang
Ya-li
YL
Wang
Ji-de
JD
D000818:Animals; D002681:China; D004269:DNA, Bacterial / Q000032:analysis / Q000235:genetics; D044923:Helicobacter hepaticus / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D007422:Intestines / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008855:Microscopy, Electron, Scanning; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S