Functional analysis reveals pleiotropic effects of rice RING-H2 finger protein gene OsBIRF1 on regulation of growth and defense responses against abiotic and biotic stresses.
RING finger proteins comprise a large family and play key roles in regulating growth/developmental processes, hormone signaling and responses to biotic and abiotic stresses in plants. A rice gene, OsBIRF1, encoding a putative RING-H2 finger protein, was cloned and identified. OsBIRF1 encodes a 396 amino acid protein belonging to the ATL family characterized by a conserved RING-H2 finger domain (C-X2-C-X15-C-X1-H-X2-H-X2-C-X10-C-X2-C), a transmembrane domain at the N-terminal, a basic amino acid rich region and a characteristic GLD region. Expression of OsBIRF1 was up-regulated in rice seedlings after treatment with benzothaidiazole, salicylic acid, l-aminocyclopropane-1-carboxylic acid and jasmonic acid, and was induced differentially in incompatible but not compatible interactions between rice and Magnaporthe grisea, the causal agent of blast disease. Transgenic tobacco plants that constitutively express OsBIRF1 exhibit enhanced disease resistance against tobacco mosaic virus and Pseudomonas syringae pv. tabaci and elevated expression levels of defense-related genes, e.g. PR-1, PR-2, PR-3 and PR-5. The OsBIRF1-overexpressing transgenic tobacco plants show increased oxidative stress tolerance to exogenous treatment with methyl viologen and H2O2, and up-regulate expression of oxidative stress-related genes. Reduced ABA sensitivity in root elongation and increased drought tolerance in seed germination were also observed in OsBIRF1 transgenic tobacco plants. Furthermore, the transgenic tobacco plants show longer roots and higher plant heights as compared with the wild-type plants, suggesting that overexpression of OsBIRF1 promote plant growth. These results demonstrate that OsBIRF1 has pleiotropic effects on growth and defense response against multiple abiotic and biotic stresses.
Liu
Huizhi
H
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Huajiachi Campus, Hangzhou, Zhejiang, People's Republic of China.
Zhang
Huijuan
H
Yang
Yayun
Y
Li
Guojun
G
Yang
Yuxia
Y
Wang
Xiao'e
X
Basnayake
B M Vindhya S
BM
Li
Dayong
D
Song
Fengming
F
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D002264:Carboxylic Acids / Q000494:pharmacology; D003517:Cyclopentanes / Q000494:pharmacology; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000494:pharmacology; D007113:Immunity, Innate; D020082:Magnaporthe / Q000254:growth & development; D008969:Molecular Sequence Data; D012275:Oryza / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D018384:Oxidative Stress; D054883:Oxylipins / Q000494:pharmacology; D010269:Paraquat / Q000494:pharmacology; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology / Q000821:virology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified; D044224:Pseudomonas syringae / Q000254:growth & development; D020156:Salicylic Acid / Q000494:pharmacology; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D013830:Thiadiazoles / Q000494:pharmacology; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000254:growth & development
D002264:Carboxylic Acids; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D013830:Thiadiazoles; C015700:benzo-1,2,3-thiadiazole; C011006:jasmonic acid; D000040:Abscisic Acid; D006861:Hydrogen Peroxide; D020156:Salicylic Acid; D010269:Paraquat