Expression of Rab27B-binding protein Slp1 in pancreatic acinar cells and its involvement in amylase secretion.
Slp1 is a putative Rab27 effector protein and implicated in intracellular membrane transport; however, the precise tissue distribution and function of Slp1 protein remain largely unknown. In this study we investigated the tissue distribution of Slp1 in mice and found that Slp1 is abundantly expressed in the pancreas, especially in the apical region of pancreatic acinar cells. Slp1 interacted with Rab27B in vivo and both proteins were co-localized on zymogen granules. Morphological analysis of fasted Slp1 knockout mice showed an increased number of zymogen granules in the pancreatic acinar cells, indicating that Slp1 is part of the machinery of amylase secretion by the exocrine pancreas.
Saegusa
Chika
C
Fukuda Initiative Research Unit, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Kanno
Eiko
E
Itohara
Shigeyoshi
S
Fukuda
Mitsunori
M
D000681:Amylases / Q000378:metabolism*; D000818:Animals; D015870:Gene Expression; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D046790:Pancreas, Exocrine / Q000166:cytology / Q000502:physiology*; D022142:Secretory Vesicles / Q000378:metabolism; D033921:Vesicular Transport Proteins; D020691:rab GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*
D008565:Membrane Proteins; C424133:Sytl1 protein, mouse; D033921:Vesicular Transport Proteins; D000681:Amylases; D020691:rab GTP-Binding Proteins