title: Critical role of p53 in histone deacetylase inhibitor-induced Epstein-Barr virus Zta expression.
abstract: The tumor suppressor gene p53 plays a central role in the maintenance of normal cell growth and genetic integrity, while its impact on the Epstein-Barr virus (EBV) life cycle remains elusive. We found that p53 is important for histone deacetylase inhibitor-induced EBV lytic gene expression in nasopharyngeal carcinoma cells. Restoration of p53 in p53-null, EBV-infected H1299 cells augments the potential for viral lytic cycle initiation. Evidence from reporter assays demonstrated that p53 contributes to the expression of the immediate-early viral Zta gene. Further analysis indicated that the DNA-binding ability of p53 and phosphorylation of Ser392 may be critical. This study provides the first evidence that p53 is involved in the regulation of EBV lytic cycle initiation.
authors.affiliation: Graduate Institute of Microbiology, College of Medicine, National Taiwan University, No. 1, Sec. 1, Ren-Ai Rd., Taipei 10051, Taiwan.
authors.forename: Shih-Shin
authors.initials: SS
authors.lastname: Chang
authors.forename: You-Chang
authors.initials: YC
authors.lastname: Lo
authors.forename: Huey-Huey
authors.initials: HH
authors.lastname: Chua
authors.forename: Hsin-Yi
authors.initials: HY
authors.lastname: Chiu
authors.forename: Shu-Chun
authors.initials: SC
authors.lastname: Tsai
authors.forename: Jen-Yang
authors.initials: JY
authors.lastname: Chen
authors.forename: Kwok-Wai
authors.initials: KW
authors.lastname: Lo
authors.forename: Ching-Hwa
authors.initials: CH
authors.lastname: Tsai
mesh_terms: D045744:Cell Line, Tumor; D005816:Genetic Complementation Test; D004854:Herpesvirus 4, Human / Q000502:physiology*; D056572:Histone Deacetylase Inhibitors; D006801:Humans; D015534:Trans-Activators / Q000096:biosynthesis*; D016159:Tumor Suppressor Protein p53 / Q000172:deficiency / Q000378:metabolism*; D014775:Virus Activation
chemical_list: C063676:BZLF1 protein, Herpesvirus 4, Human; D056572:Histone Deacetylase Inhibitors; D015534:Trans-Activators; D016159:Tumor Suppressor Protein p53