title: [Comparison between culture media and procedures to detect Streptococcus agalactiae in pregnant women].
abstract: Detection of Streptococcus agalactiae in pregnant women's vagina and rectum and intrapartum antibiotic prophylaxis administered to colonized women are currently recommended to prevent neonatal precocious infections by this organism. In turn, it is very important to select the culture media and adequate sample collection site for S. agalactiae detection in colonized women. To standardize this methods in laboratory, different culture media and procedures for S. agalactiae recovery in pregnant women with obstetric and gynecologic complications were compared. Vaginorectal and endocervical swab specimens were collected from 60 pregnant women. The first sample was placed onto selective Columbia blood agar directly and onto selective Todd-Hewitt broth incubated at 37 degrees C and subcultured onto selective Columbia blood agar at 4 and 18 hours. The second sample was cultured on selective Columbia blood agar. Both culture media were incubated in a microaerophilic atmosphere at 37 degrees C from 24 to 48 hours. S. agalactiae was identified using conventional tests. 21 patients colonized with S. agalactiae were detected using vaginoanorectal samples. 19 (31.7%) patients tested positive for S. agalactiae through the culture of specimens directly onto selective Columbia blood agar; 21 (35%) and 20 (33%) patients were found to be positive for S. agalactiae by the selective Todd-Hewitt broth at 4 and 18 hours, respectively. Only one patient tested positive for S. agalactiae in the endocervical tract. The results show that the three procedures followed for S. agalactiae recovery are effective. Nevertheless, the procedure in which the sample was placed directly onto selective Columbia blood agar permits reducing costs and the time for bacteria identification. On the other hand, the vaginoanorectal swab was the best sample to detect colonization by S. agalactiae in pregnant women.
authors.affiliation: Departamento de Bioanálisis Clínico, Facultad de Farmacia y Bioanálisis, Universidad de los Andes, Mérida, Venezuela. tulia@ula.ve
authors.forename: Tulia
authors.initials: T
authors.lastname: M Díaz
authors.forename: Beatriz
authors.initials: B
authors.lastname: M Nieves
mesh_terms: D001431:Bacteriological Techniques; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000175:diagnosis* / Q000382:microbiology; D012007:Rectum / Q000382:microbiology; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013292:Streptococcus agalactiae / Q000254:growth & development / Q000302:isolation & purification*; D014621:Vagina / Q000382:microbiology
chemical_list: D003470:Culture Media