title: Intramanchette transport during primate spermiogenesis: expression of dynein, myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein, and Rab27b in the manchette during human and monkey spermiogenesis.
abstract: OBJECTIVE
  To show whether molecular motor dynein on a microtubule track, molecular motor myosin Va, motor recruiter myosin Va, VIIa-Rab27a/b interacting protein (MyRIP), and vesicle receptor Rab27b on an F-actin track were present during human and monkey spermiogenesis involving intramanchette transport (IMT).
  
  
  METHODS
  Spermiogenic cells were obtained from three men with obstructive azoospermia and normal adult cynomolgus monkey (Macaca fascicularis). Immunocytochemical detection and reverse transcription-polymerase chain reaction (RT-PCR) analysis of the proteins were carried out. Samples were analyzed by light microscope.
  
  
  RESULTS
  Using RT-PCR, we found that dynein, myosin Va, MyRIP and Rab27b were expressed in monkey testis. These proteins were localized to the manchette, as shown by immunofluorescence, particularly during human and monkey spermiogenesis.
  
  
  CONCLUSIONS
  We speculate that during primate spermiogenesis, those proteins that compose microtubule-based and actin-based vesicle transport systems are actually present in the manchette and might possibly be involved in intramanchette transport.
authors.affiliation: Department of Obstetrics and Gynecology, Tohoku University School of Medicine, Sendai, Miyagi, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Hayasaka
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Terada
authors.forename: Kichiya
authors.initials: K
authors.lastname: Suzuki
authors.forename: Haruo
authors.initials: H
authors.lastname: Murakawa
authors.forename: Ikuo
authors.initials: I
authors.lastname: Tachibana
authors.forename: Tadashi
authors.initials: T
authors.lastname: Sankai
authors.forename: Takashi
authors.initials: T
authors.lastname: Murakami
authors.forename: Nobuo
authors.initials: N
authors.lastname: Yaegashi
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Okamura
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D000818:Animals; D001692:Biological Transport / Q000502:physiology; D004398:Dyneins / Q000378:metabolism*; D006801:Humans; D008252:Macaca fascicularis; D008297:Male; D008870:Microtubules / Q000378:metabolism*; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism*; D009218:Myosins / Q000378:metabolism; D013087:Spermatids / Q000166:cytology / Q000378:metabolism*; D013091:Spermatogenesis / Q000502:physiology*; D013737:Testis / Q000166:cytology / Q000378:metabolism; D022161:Transport Vesicles / Q000502:physiology; D033921:Vesicular Transport Proteins / Q000378:metabolism*; D020691:rab GTP-Binding Proteins / Q000378:metabolism*
chemical_list: D000199:Actins; C501262:MYRIP protein, human; D033921:Vesicular Transport Proteins; C077511:MYO5A protein, human; D024701:Myosin Type V; C512960:Rab27B protein, human; D018995:Myosin Heavy Chains; D009218:Myosins; D004398:Dyneins; D020691:rab GTP-Binding Proteins