Monocyte-derived dendritic cells from HLA-matched allogeneic donors showed a greater ability to induce leukemic cell-specific T cells in comparison to leukemic cell-derived dendritic cells or monocyte-derived dendritic cells from AML patients.
We investigated the usefulness of allogeneic monocyte-derived dendritic cells (allogeneic mDCs) pulsed with leukemic lysates in acute myeloid leukemia (AML). Allogeneic mDCs showed higher expressions of several molecules (HLA-DR, CD80, CD83 or CD86), higher production of IL-12 and higher capacity to stimulate allogeneic T cells compared to both leukemic DCs and autologous mDCs. Autologous T cells primed by allogeneic mDCs displayed a larger number of interferon-gamma-secreting cells against leukemic cells than those primed by either leukemic DCs or autologous mDCs. These results suggest that monocyte-derived DCs from HLA-matched allogeneic donors can be used as an alternative to generate leukemia-specific cytotoxic T cells and to overcome the limitation of leukemic DCs or autologous mDCs.
Lee
Je-Jung
JJ
Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun, Jeonnam, South Korea.
Choi
Bo-Hwa
BH
Kang
Hyun-Kyu
HK
Kim
Sang-Ki
SK
Nam
Jong-Hee
JH
Yang
Deok-Hwan
DH
Kim
Yeo-Kyeoung
YK
Kim
Hyeoung-Joon
HJ
Chung
Ik-Joo
IJ
D015703:Antigens, CD / Q000276:immunology; D018122:B7-1 Antigen / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000276:immunology / Q000378:metabolism; D006648:Histocompatibility; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014019:Tissue Donors; D000097802:CD83 Antigen
D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006680:HLA Antigens; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D018664:Interleukin-12; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen