title: Protecting, patterning, and scaffolding supported lipid membranes using carbohydrate glasses.
abstract: Disaccharides are known to protect sensitive biomolecules against stresses caused by dehydration, both in vivo and in vitro. Here we demonstrate how interfacial accumulation of trehalose can be used to (1) produce rugged supported lipid bilayers capable of near total dehydration; (2) enable spatial patterning of membrane micro-arrays; and (3) form stable bilayers on otherwise lipophobic substrates (e.g., metal transducers) thus affording protecting, patterning, and scaffolding of lipid bilayers.
authors.affiliation: Department of Applied Science, University of California-Davis, 1 Shields Ave., Davis, CA 95616, USA. aeoliver@ucdavis.edu
authors.forename: Ann E
authors.initials: AE
authors.lastname: Oliver
authors.forename: Eric L
authors.initials: EL
authors.lastname: Kendall
authors.forename: Michael C
authors.initials: MC
authors.lastname: Howland
authors.forename: Babak
authors.initials: B
authors.lastname: Sanii
authors.forename: Andrew P
authors.initials: AP
authors.lastname: Shreve
authors.forename: Atul N
authors.initials: AN
authors.lastname: Parikh
mesh_terms: D002241:Carbohydrates / Q000737:chemistry*; D005898:Glass / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry*; D008670:Metals / Q000737:chemistry; D036103:Nanotechnology / Q000379:methods*; D010743:Phospholipids / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014159:Transducers; D014199:Trehalose / Q000737:chemistry; D014867:Water / Q000737:chemistry
chemical_list: D002241:Carbohydrates; D008051:Lipid Bilayers; D008670:Metals; D010743:Phospholipids; D014867:Water; D014199:Trehalose