[DNA complexes with polycations used for delivery of DNA into cells].
The formation and physicochemical properties of high-molecular thymus and plasmid DNA complexes with synthetic polymers based on (dimethyl-amino)ethyl methacrylate (DMAEM), (diethyl-amino)ethyl methacrylate (DEAEM), and polyvinyl amine (PVA) were investigated in solutions of different ionic strength by low-gradient viscometry, electrophoresis, circular dichroism, spectrophotometry, and dynamic light scattering. The toxicity of complexes in T98G cells was studied. It was shown that, when the ratio of polycations to DNA charged groups concentration (N+/P) reaches values > 1, DNA condensation occurs. It is accompanied by increasing optical density of solutions. Changes in DNA size after condensation were estimated. Phase diagrams of systems DNA/polycation in the presence of NaCl were obtained. It was shown by MTT-analysis that DNA complexes with polycations in the range of concentrations used have low toxicity.
Kas'ianenko
N A
NA
Zakharova
N B
NB
Mukhin
D A
DA
Slita
A V
AV
Nazarova
O V
OV
Leont'eva
E A
EA
Panarin
E F
EF
D000588:Amines / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002460:Cell Line; D004247:DNA / Q000737:chemistry* / Q000633:toxicity; D006801:Humans; D008689:Methacrylates / Q000737:chemistry*; D009757:Nylons; D009994:Osmolar Concentration; D010957:Plasmids; D011073:Polyamines / Q000737:chemistry* / Q000633:toxicity; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*; D012996:Solutions; D013950:Thymus Gland / Q000737:chemistry; D014162:Transfection / Q000379:methods*
D000588:Amines; D004247:DNA; D008689:Methacrylates; D009757:Nylons; D011073:Polyamines; D000071228:Polyelectrolytes; D011108:Polymers; D011145:Polyvinyls; D012996:Solutions; C407037:poly(2-(dimethylamino)ethyl methacrylate); C519537:diethylaminoethyl methacrylate