Detection of lectin-glycan interaction using high resolution surface plasmon resonance.
We report the real-time and label-free detection of direct disaccharide binding to a lectin using a differential surface plasmon resonance detection method that allows for measurement of nanomolar concentrations of disaccharides.
Foley
Kyle J
KJ
Department of Electrical Engineering, Arizona State University, Tempe, AZ 85282-5706, USA.
Forzani
Erica S
ES
Joshi
Lokesh
L
Tao
Nongjian
N
D037102:Lectins / Q000737:chemistry*; D036103:Nanotechnology; D011134:Polysaccharides / Q000737:chemistry*; D011485:Protein Binding; D020349:Surface Plasmon Resonance / Q000379:methods*
D037102:Lectins; D011134:Polysaccharides