title: Phenotype analysis of lymphoid cells in Marek's disease of CD4(+) or CD8(+) T-cell-deficient chickens: Occurrence of double negative T-cell tumour.
abstract: Chickens rendered either CD4(+) or CD8(+) T-cell-deficient by thymectomy and subsequent inoculation of anti-CD4 or anti-CD8 monoclonal antibody (mAb), were infected with Marek's disease herpesvirus (MDV). All the non-treated control (4/4) and CD8-deficient chickens (5/5) formed gross tumours. However, one of three CD4(+) T-cell-deficient chickens developed gross tumours. Histologically, all chickens had tumourous lesions in the liver and nerve fibres. In CD4(+) T-cell-deficient chickens, the phenotypes were CD3(+)CD4(-)CD8(-)Vss1-alphassTCR(+) T-cells and CD3(-)CD4(-)CD8(-) cells. These findings indicate that the double negative T-cells (CD3(+) CD4(-) CD8(-)) and CD3(-) CD4(-) CD8(-) cells are transformed morphologically in addition to CD3(+) CD4(+) CD8(-) T-cells by MDV infection.
authors.affiliation: Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan.
authors.forename: K
authors.initials: K
authors.lastname: Okada
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.forename: K
authors.initials: K
authors.lastname: Murakami
authors.forename: S
authors.initials: S
authors.lastname: Chiba
authors.forename: T
authors.initials: T
authors.lastname: Morimura
authors.forename: M
authors.initials: M
authors.lastname: Hattori
authors.forename: M
authors.initials: M
authors.lastname: Goryo
authors.forename: M
authors.initials: M
authors.lastname: Onuma