Overexpression of the PDCD2-like gene results in inhibited TNF-alpha production in activated Daudi cells.
Both PDCD2 and PDCD2-like proteins have PDCD2_C terminal domains, but their functions are still unclear. After analysis of their expression in Gene Expression Omnibus, we hypothesized that they played a role in inflammation. In Daudi cells exposed to lipopolysaccharides, PDCD2-like RNA was upregulated, whereas PDCD2 was downregulated. Overexpression of PDCD2-like gene effectively attenuated tumor necrosis factor (TNF)-alpha release but elevated interleukin (IL)-6 and PDCD2 expression induced by lipopolysaccharide (LPS). It is possible that both proteins have anti-inflammatory effects like IL-6. The results have implications for understanding the function of PDCD2/PDCD2-like proteins and may help in the development of novel anti-inflammatory drugs.
Chen
Q
Q
School of Radiology and Public Health, Soochow University, Suzhou 215123, People's Republic of China.
Yan
C-Q
CQ
Liu
F-J
FJ
Tong
J
J
Miao
S-L
SL
Chen
J-P
JP
D017304:Annexin A5 / Q000032:analysis; D017209:Apoptosis; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D018274:Electroporation; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology / Q000494:pharmacology; D012333:RNA, Messenger / Q000032:analysis; D014157:Transcription Factors / Q000378:metabolism; D014162:Transfection; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D015854:Up-Regulation
D017304:Annexin A5; D051017:Apoptosis Regulatory Proteins; C508600:IL6 protein, human; D015850:Interleukin-6; D008070:Lipopolysaccharides; C094601:PDCD2 protein, human; D012333:RNA, Messenger; D014157:Transcription Factors; D014409:Tumor Necrosis Factor-alpha