Atmospheric pressure photoionization mass spectrometry of oligodeoxyribonucleotides.
Small oligonucleotides (di- and trimers) were investigated by atmospheric pressure photoionization (APPI) with focus on the fragmentation mechanisms. The fragmentation patterns of these biomolecular ions have been monitored under dopant-assisted photoionization (DA-APPI) conditions. Our results reveal new aspects of the gas- phase chemistry of ions formed from such biomolecules. They illustrate that the reaction between low-energy electrons released from photoionization processes and di- and trinucleotides lies in dissociative electron attachment processes leading to phosphodiester bond cleavages and to the formation of numerous fragments in the ion source. The conditions of DA-APPI, which involve protic solvents and atmospheric pressure conditions, seem to be relevant for the study of radiation damages to biological molecules.
Bagag
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Laboratoire de Spectrométrie de Masse, ICSN-CNRS, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
Giuliani
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Laprévote
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D000838:Anions / Q000737:chemistry; D001274:Atmospheric Pressure; D053960:DNA Breaks; D004583:Electrons; D013058:Mass Spectrometry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010777:Photochemistry
D000838:Anions; D009838:Oligodeoxyribonucleotides