title: Influence of vanadate on migrating fibroblast orientation within a fibrin matrix.
abstract: Treating rats with vanadate, a nonspecific inhibitor of protein tyrosine phosphatases, optimizes the uniform packing of collagen fiber bundles in wound granulation tissue and doubles wound breaking strength in rat incisional wounds. The speculation is vanadate optimizes the packing of collagen fiber bundles through the orientation of newly arrived wound fibroblasts in the fibrin clot filling the defect. Segments of 14 day chick embryo tendons were placed on fibrin clots and maintained in organ culture with and without 30 microM vanadate. On day 7 explants were examined histologically and biochemically. Tendon fibroblast outgrowth from untreated explants migrated in a random fashion, while fibroblasts from vanadate-treated explants migrated out in linear arrays. Fibroblasts were elongated by 20% form vanadate treated explant compared to controls. Myosin ATPase, required for optimal cell motility, is optimized by the phosphorylation of its myosin light chain (MLC). Western blot analysis of lysates from the fibroblasts that migrated into the fibrin showed vanadate increased MLC-P levles. These findings support the notion that vanadate promotes the deposition of regular, parallel collagen fiber bundles by advancing the orientation of fibroblasts in parallel linear arrays early in the wound repair process.
authors.affiliation: Division of Plastic Surgery, Milton S. Hershey Medical Center, Hershey, Pennsylvania, USA.
authors.forename: Michael Y
authors.initials: MY
authors.lastname: Lee
authors.forename: H Paul
authors.initials: HP
authors.lastname: Ehrlich
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002465:Cell Movement / Q000187:drug effects*; D002642:Chick Embryo; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000737:chemistry; D005337:Fibrin / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008856:Microscopy, Fluorescence; D018994:Myosin Light Chains / Q000187:drug effects; D009924:Organ Culture Techniques; D010766:Phosphorylation; D013710:Tendons / Q000166:cytology; D014638:Vanadates / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects* / Q000502:physiology
chemical_list: D004791:Enzyme Inhibitors; D018994:Myosin Light Chains; D014638:Vanadates; D005337:Fibrin