title: Validation of a high performance liquid chromatography method for sunscreen determination in cosmetics.
abstract: The aim of this research was the development and validation of a high performance liquid chromatography (HPLC) method for the simultaneous and quantitative determination of benzophenone-3, octyl methoxycinnamate and octyl salicylate contained in sunscreen emulsions. The separation and quantitative determination was achieved using a LiChrospher((R)) 100 RP-18 (5 microm) Merck column, a mobile phase constituted of methanol/water (85/15, v/v), a flow-rate of 1.0 mL min(-1) and UV detection at 310 nm. The correlation coefficients and percentage of recovery for benzophenone-3, octyl methoxycinnamate and octyl salicylate were 0.9999 and 99.46%; 0.9995 and 98.85%; 0.9998 and 98.84%, respectively. The relative standard deviations (RSD) for commercial samples were between 0.50 and 0.70%.
authors.affiliation: Department of Pharmacy, Faculty of Pharmaceutical Sciences, University of São Paulo, Caixa Postal 66083, CEP 05315-970, São Paulo, SP, Brazil.
authors.forename: E A
authors.initials: EA
authors.lastname: Dutra
authors.forename: E R M
authors.initials: ER
authors.lastname: Kedor-Hackmann
authors.forename: M I R M
authors.initials: MI
authors.lastname: Santoro