Examination of oral cancer biomarkers by tissue microarray analysis.
OBJECTIVE
To validate the DNA microarray results on a subset of genes that could potentially serve as biomarkers of oral squamous cell carcinoma (OSCC) by examining their expression with an alternate quantitative method and by assessing their protein levels.


METHODS
Based on DNA microarray data from our laboratory and data reported in the literature, we identified 6 potential biomarkers of OSCC to investigate further. We used quantitative real-time polymerase chain reaction to examine expression changes of CDH11, MMP3, SPARC, POSTN, TNC, and TGM3 in OSCC and histologically normal control tissues. We further examined validated markers at the protein level by immunohistochemical analysis of OSCC tissue microarray sections.


RESULTS
Quantitative real-time polymerase chain reaction analysis revealed upregulation of CDH11, SPARC, POSTN, and TNC gene expression and decreased TGM3 expression in OSCC tissue compared with control tissue; MMP3 was not found to be differentially expressed. In tissue microarray immunohistochemical analyses, SPARC (secreted protein, acidic, rich in cysteine), periostin, and tenascin C exhibited increased protein expression in tumor tissue compared with control tissue, and their expression was primarily localized within tumor-associated stroma rather than tumor epithelium. Conversely, transglutaminase 3 protein expression was found only within keratinocytes in control tissue and was significantly downregulated in cancer cells.


CONCLUSIONS
Of 6 potential gene markers of OSCC, initially identified by DNA microarray analyses, differential expression of CDH11, SPARC, POSTN, TNC, and TGM3 were validated by quantitative real-time polymerase chain reaction. Differential expression and localization of proteins encoded by SPARC, POSTN, TNC, and TGM3 were clearly shown by tissue microarray immunohistochemical analysis.
Choi
Peter
P
Department of Otolaryngology-Head and Neck Surgery, University ofWashington, Seattle, WA, USA.
Jordan
C Diana
CD
Mendez
Eduardo
E
Houck
John
J
Yueh
Bevan
B
Farwell
D Gregory
DG
Futran
Neal
N
Chen
Chu
C
D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D015820:Cadherins / Q000378:metabolism; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D016022:Case-Control Studies; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D006801:Humans; D007150:Immunohistochemistry; D016014:Linear Models; D019278:Matrix Metalloproteinase 3 / Q000378:metabolism; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D020411:Oligonucleotide Array Sequence Analysis; D015676:Osteonectin / Q000378:metabolism; D015203:Reproducibility of Results; D019063:Tenascin / Q000378:metabolism; D046888:Tissue Array Analysis; D011503:Transglutaminases / Q000378:metabolism
D014408:Biomarkers, Tumor; D015820:Cadherins; D015815:Cell Adhesion Molecules; D015676:Osteonectin; C512835:POSTN protein, human; D019063:Tenascin; C086960:osteoblast cadherin; C498712:TGM3 protein, human; D011503:Transglutaminases; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3