title: microRNA-7 inhibits the epidermal growth factor receptor and the Akt pathway and is down-regulated in glioblastoma.
abstract: microRNAs are noncoding RNAs inhibiting expression of numerous target genes, and a few have been shown to act as oncogenes or tumor suppressors. We show that microRNA-7 (miR-7) is a potential tumor suppressor in glioblastoma targeting critical cancer pathways. miR-7 potently suppressed epidermal growth factor receptor expression, and furthermore it independently inhibited the Akt pathway via targeting upstream regulators. miR-7 expression was down-regulated in glioblastoma versus surrounding brain, with a mechanism involving impaired processing. Importantly, transfection with miR-7 decreased viability and invasiveness of primary glioblastoma lines. This study establishes miR-7 as a regulator of major cancer pathways and suggests that it has therapeutic potential for glioblastoma.
authors.affiliation: Division of Neuro-Oncology, Neurology Department, University of Virginia Health System, Charlottesville, Virginia, USA.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Kefas
authors.forename: Jakub
authors.initials: J
authors.lastname: Godlewski
authors.forename: Laurey
authors.initials: L
authors.lastname: Comeau
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Li
authors.forename: Roger
authors.initials: R
authors.lastname: Abounader
authors.forename: Michael
authors.initials: M
authors.lastname: Hawkinson
authors.forename: Jeongwu
authors.initials: J
authors.lastname: Lee
authors.forename: Howard
authors.initials: H
authors.lastname: Fine
authors.forename: E Antonio
authors.initials: EA
authors.lastname: Chiocca
authors.forename: Sean
authors.initials: S
authors.lastname: Lawler
authors.forename: Benjamin
authors.initials: B
authors.lastname: Purow
mesh_terms: D020413:3' Untranslated Regions; D001932:Brain Neoplasms / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002469:Cell Separation; D015536:Down-Regulation; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic; D005909:Glioblastoma / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D014162:Transfection
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt