Silencing of YY1 downregulates RIZ1 promoter in human osteosarcoma.
RIZ1 isoform, but not RIZ2, is commonly silenced in many types of tumors. In osteosarcoma cells, RIZ1 protein is very abundant. The silencing of YY1 protein, a recent target gene in osteosarcoma cells, reduced the expression of RIZ1 protein. Here we show that RIZ1 overexpression is a transcriptional event documented by Western blot, RT-PCR, and promoter assays. YY1 protein binds and cooperates to positive regulation of the RIZ1 promoter and its presence reduced the dimethyl lysine 9 histone 3 by chromatin immunoprecipitation assays. These results indicate that overexpression of YY1 in osteosarcoma cells plays a key role in positive regulation of RIZ1. The coexpression of RIZ1/YY1 proteins suggests a tandem regulatory mechanism in human osteosarcoma cells and tissues.
Abbondanza
Ciro
C
Department of General Pathology, 1st School of Medicine, II University of Naples, Naples 80138, Italy.
de Nigris
Filomena
F
De Rosa
Caterina
C
Rossiello
Raffaele
R
Puca
Giovanni Alfredo
GA
Napoli
Claudio
C
D001483:Base Sequence; D001859:Bone Neoplasms / Q000235:genetics*; D045744:Cell Line, Tumor; D047369:Chromatin Immunoprecipitation; D004268:DNA-Binding Proteins / Q000235:genetics*; D015536:Down-Regulation; D020868:Gene Silencing; D011495:Histone-Lysine N-Methyltransferase; D006801:Humans; D007150:Immunohistochemistry; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D012516:Osteosarcoma / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014157:Transcription Factors / Q000235:genetics*; D050986:YY1 Transcription Factor / Q000235:genetics / Q000502:physiology*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors; D050986:YY1 Transcription Factor; C494698:YY1 protein, human; D011495:Histone-Lysine N-Methyltransferase; C093563:PRDM2 protein, human