Functional characterization of domains in AtTRB1, a putative telomere-binding protein in Arabidopsis thaliana.
Telomeres are nucleoprotein structures ensuring the stability of eukaryotic chromosome ends. Two protein families, TRFL (TFL-Like) and SMH (Single-Myb-Histone), containing a specific telobox motif in their Myb domain, have been identified as potential candidates involved in a functional nucleoprotein structure analogous to human "shelterin" at plant telomeres. We analyze the DNA-protein interaction of the full-length and truncated variants of AtTRB1, a SMH-family member with a typical structure: N-terminal Myb domain, central H1/5 domain and C-terminal coiled-coil. We show that preferential interaction of AtTRB1 with double-stranded telomeric DNA is mediated by the Myb domain, while the H1/5 domain is involved in non-specific DNA-protein interaction and in the multimerization of AtTRB1.
Mozgová
Iva
I
Department of Functional Genomics and Proteomics, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 5, CZ-62500 Brno, Czech Republic.
Schrumpfová
Petra Procházková
PP
Hofr
Ctirad
C
Fajkus
Jirí
J
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018744:DNA, Plant / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D006657:Histones / Q000378:metabolism; D006801:Humans; D020626:Oncogene Proteins v-myb / Q000378:metabolism; D011485:Protein Binding; D016615:Telomere / Q000378:metabolism*
D029681:Arabidopsis Proteins; C531202:AtTRB1 protein, Arabidopsis; D018744:DNA, Plant; D004268:DNA-Binding Proteins; D006657:Histones; D020626:Oncogene Proteins v-myb