title: Monocyte-derived dendritic cells from HLA-matched allogeneic donors showed a greater ability to induce leukemic cell-specific T cells in comparison to leukemic cell-derived dendritic cells or monocyte-derived dendritic cells from AML patients.
abstract: We investigated the usefulness of allogeneic monocyte-derived dendritic cells (allogeneic mDCs) pulsed with leukemic lysates in acute myeloid leukemia (AML). Allogeneic mDCs showed higher expressions of several molecules (HLA-DR, CD80, CD83 or CD86), higher production of IL-12 and higher capacity to stimulate allogeneic T cells compared to both leukemic DCs and autologous mDCs. Autologous T cells primed by allogeneic mDCs displayed a larger number of interferon-gamma-secreting cells against leukemic cells than those primed by either leukemic DCs or autologous mDCs. These results suggest that monocyte-derived DCs from HLA-matched allogeneic donors can be used as an alternative to generate leukemia-specific cytotoxic T cells and to overcome the limitation of leukemic DCs or autologous mDCs.
authors.affiliation: Department of Hematology-Oncology, Chonnam National University Hwasun Hospital, Hwasun, Jeonnam, South Korea.
authors.forename: Je-Jung
authors.initials: JJ
authors.lastname: Lee
authors.forename: Bo-Hwa
authors.initials: BH
authors.lastname: Choi
authors.forename: Hyun-Kyu
authors.initials: HK
authors.lastname: Kang
authors.forename: Sang-Ki
authors.initials: SK
authors.lastname: Kim
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Nam
authors.forename: Deok-Hwan
authors.initials: DH
authors.lastname: Yang
authors.forename: Yeo-Kyeoung
authors.initials: YK
authors.lastname: Kim
authors.forename: Hyeoung-Joon
authors.initials: HJ
authors.lastname: Kim
authors.forename: Ik-Joo
authors.initials: IJ
authors.lastname: Chung
mesh_terms: D015703:Antigens, CD / Q000276:immunology; D018122:B7-1 Antigen / Q000276:immunology; D051940:B7-2 Antigen / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology; D006680:HLA Antigens / Q000032:analysis; D006684:HLA-DR Antigens / Q000276:immunology / Q000378:metabolism; D006648:Histocompatibility; D006801:Humans; D007136:Immunoglobulins / Q000276:immunology; D018664:Interleukin-12 / Q000276:immunology; D015470:Leukemia, Myeloid, Acute / Q000276:immunology* / Q000473:pathology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D008562:Membrane Glycoproteins / Q000276:immunology; D009000:Monocytes / Q000276:immunology*; D010641:Phenotype; D013601:T-Lymphocytes / Q000276:immunology*; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology; D014019:Tissue Donors; D000097802:CD83 Antigen
chemical_list: D015703:Antigens, CD; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006680:HLA Antigens; D006684:HLA-DR Antigens; D007136:Immunoglobulins; D018664:Interleukin-12; D008562:Membrane Glycoproteins; D000097802:CD83 Antigen