title: In vitro study of mutagenic potential of Bidens pilosa Linné and Mikania glomerata Sprengel using the comet and micronucleus assays.
abstract: Teas of Bidens pilosa and Mikania glomerata are popularly consumed to medicinal ends. The capacity to induce DNA damages and mutagenic effects of these teas were evaluated, in vitro, on HTC cells, with comet assay and micronucleus test. The teas tested at various doses were prepared differently: infusion of Mikania glomerata (IM) and Bidens pilosa (IB), macerate of Mikania glomerata in 80% ethanol (MM80) and decoction of Bidens pilosa (DB). In IM and MM80, the quantity of coumarin was determined by high-performance liquid chromatography (HPLC) with UV detection. Methylmethanesulfonate was utilized as positive control, phosphate-buffered saline as negative control, 80% ethanol as solvent control and 2-aminoanthracene as drug metabolism control. The comet assay demonstrated genotoxic effects for both plants. The genotoxic potential of IB was upper than DB, showing dose-response. In the MN test, excepting IM 40 microL/mL, all treatments was not mutagenic. The effects did not show direct relation with cumarin quantity present in IM and MM80. The results demonstrated DNA damages at the highest concentrations of alcoholic macerate (10 and 20 microL/mL) and infusion of Mikania glomerata (20 and 40 microL/mL) and of Bidens pilosa infusion (40 microL/mL). Thus, both dose and preparation-form suggest caution in the phytotherapeutic use of these plants.
authors.affiliation: Departamento de Biologia Geral, Universidade Estadual de Londrina, Campus Universitário, Londrina, PR, Brazil.
authors.forename: Ronaldo de Jesus
authors.initials: Rde J
authors.lastname: Costa
authors.forename: Andréa
authors.initials: A
authors.lastname: Diniz
authors.forename: Mário Sérgio
authors.initials: MS
authors.lastname: Mantovani
authors.forename: Berenice Quinzani
authors.initials: BQ
authors.lastname: Jordão
mesh_terms: D000818:Animals; D031176:Bidens / Q000737:chemistry / Q000633:toxicity*; D045744:Cell Line, Tumor; D002851:Chromatography, High Pressure Liquid; D020552:Comet Assay / Q000379:methods; D003374:Coumarins / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity; D004305:Dose-Response Relationship, Drug; D008113:Liver Neoplasms / Q000378:metabolism; D015162:Micronucleus Tests / Q000379:methods; D035742:Mikania / Q000737:chemistry / Q000633:toxicity*; D009153:Mutagens / Q000008:administration & dosage / Q000302:isolation & purification / Q000633:toxicity*; D051381:Rats; D013662:Tea / Q000737:chemistry / Q000633:toxicity*
chemical_list: D003374:Coumarins; D009153:Mutagens; D013662:Tea; C030123:coumarin