Usefulness of Mycobacterium tuberculosis molecular typing in a tuberculosis low-endemic agro-industrial setting of Brazil.
To highlight the transmission and major phylogenetic clades of Mycobacterium tuberculosis, a retrospective study was carried out at two health facilities in a small agro-industrial area in Sao Paulo, Brazil, that has a low tuberculosis incidence rate. IS6110-RFLP and spoligotyping were performed on the isolates, with the former revealing that 31.3% (35/112) of strains were clustered. Epidemiological links were found in 16 of the 35 clustered patients and were associated with transmission among patients living in public housing. Spoligotyping grouped 62.8% of the strains. The T genetic family predominated among the isolates. Of interest is that five strains had a pattern characteristic of African or Asian origin (ST535), and two others were of the rare localized type ST1888 (BRA, VEN). In addition, three new types--1889, 1890, and 1891--were identified. Spoligotyping showed that some ST may be circulating to or from Brazil, and RFLP revealed ongoing transmission in inadequately ventilated public-housing buildings. This may point to a failure in tuberculosis control policy.
Malaspina
Ana Carolina
AC
Faculdade de Ciencias Farmaceuticas, Universidade Estadual Paulista, Sao Paulo, Brazil.
Cavalcanti
Hebe Rodrigues
HR
Leite
Clarice Queico Fujimaro
CQ
Machado
Silvia Maria Almeida
SM
Viana
Brunilde Helena Jung
BH
Silva
Rosangela Maria Gaspareto
RM
Hage
Eduardo Ferraz
EF
Figueiredo
Walter Machado
WM
Marques
Elisabeth
E
Ferrazoli
Lucilaine
L
Arbex
Marcos
M
Lessi
Marcio
M
Fonseca
Leila S
LS
Rigouts
Leen
L
Saad
Maria Helena Féres
MH
D001938:Brazil / Q000453:epidemiology; D004251:DNA Transposable Elements; D005260:Female; D006801:Humans; D015994:Incidence; D008297:Male; D009169:Mycobacterium tuberculosis / Q000145:classification* / Q000235:genetics*; D009841:Oligonucleotides / Q000032:analysis; D012150:Polymorphism, Restriction Fragment Length; D011638:Public Housing; D014397:Tuberculosis, Pulmonary / Q000453:epidemiology* / Q000382:microbiology
D004251:DNA Transposable Elements; D009841:Oligonucleotides