Gastroprotective activity of the chloroform extract of the roots from Arctium lappa L.
Arctium lappa L. is used in folk medicine as a diuretic, depurative and digestive stimulant and in dermatological conditions. The objective of this study was to evaluate the effect and the possible mechanisms involved in the gastroprotective effects of a chloroform extract (CE) of the roots from A. lappa and its fractions. Oral pretreatment with CE (10, 30 and 100 mg kg(-1)) significantly reduced gastric lesions induced by ethanol by 61%, 70% and 76%, respectively. Oral administration of CE (100 mg kg(-1) per day for 7 days) reduced the chronic gastric ulceration induced by acetic acid by 52%. Intraduodenal CE (100, 300 and 600 mg kg(-1)) reduced the total acidity of gastric secretion by 22%, 22% and 33%, respectively, while i.p. administration (10, 30 and 100 mg kg(-1)) inhibited total acidity by 50%, 60% and 67%, respectively. In-vitro, CE inhibited H+, K+ -ATPase activity with an EC50 of 53 microgmL(-1) and fraction A (30 and 100 microgmL(-1)) reduced this by 48% and 89%, respectively. CE had no effect on gastrointestinal motility. CE (250 microgmL(-1)) and fraction B (100 and 250 microgmL(-1)) had free-radical scavenging ability, inhibiting 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical activity by 50%, 20% and 55%, respectively. Collectively, the results show that the CE protects animals from gastric lesions by reducing gastric acid secretion via inhibition of gastric H+, K+ -ATPase.
Dos Santos
Ana C
AC
Department of Pharmacology, Sector of Biological Sciences, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Baggio
Cristiane H
CH
Freitas
Cristina S
CS
Lepieszynski
Juliana
J
Mayer
Bárbara
B
Twardowschy
André
A
Missau
Fabiana C
FC
dos Santos
Elide P
EP
Pizzolatti
Moacir G
MG
Marques
Maria C A
MC
D000818:Animals; D000897:Anti-Ulcer Agents / Q000008:administration & dosage / Q000494:pharmacology*; D031170:Arctium / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005260:Female; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000494:pharmacology; D005744:Gastric Acid / Q000378:metabolism; D005753:Gastric Mucosa / Q000187:drug effects / Q000378:metabolism; D005769:Gastrointestinal Motility / Q000187:drug effects; D008519:Medicine, Traditional; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018517:Plant Roots; D054328:Proton Pump Inhibitors; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000517:prevention & control*
D000897:Anti-Ulcer Agents; D016166:Free Radical Scavengers; D010936:Plant Extracts; D054328:Proton Pump Inhibitors