Eukaryotic elongation factor 1A interacts with Turnip mosaic virus RNA-dependent RNA polymerase and VPg-Pro in virus-induced vesicles.
Eukaryotic elongation factor 1-alpha (eEF1A) was identified as an interactor of Turnip mosaic virus (TuMV) RNA-dependent RNA polymerase (RdRp) and VPg-protease (VPg-Pro) using tandem affinity purification and/or in vitro assays. Subcellular fractionation experiments revealed that the level of eEF1A substantially increased in membrane fractions upon TuMV infection. Replication of TuMV occurs in cytoplasmic membrane vesicles, which are induced by 6K-VPg-Pro. Confocal microscopy indicated that eEF1A was included in these vesicles. To confirm that eEF1A was found in replication vesicles, we constructed an infectious recombinant TuMV that contains an additional copy of the 6K protein fused to the green fluorescent protein (GFP). In cells infected with this recombinant TuMV, fluorescence emitted by 6KGFP was associated with cytoplasmic membrane vesicles that contained VPg-Pro, the eukaryotic initiation factor (iso) 4E, the poly(A)-binding protein, the heat shock cognate 70-3 protein, and eEF1A. These results suggest that TuMV-induced membrane vesicles host at least three plant translation factors in addition to the viral replication proteins.
Thivierge
Karine
K
Department of Plant Science, McGill University, 21,111 Lakeshore, Ste-Anne-de-Bellevue, Quebec, Canada H9X 3V9.
Cotton
Sophie
S
Dufresne
Philippe J
PJ
Mathieu
Isabelle
I
Beauchemin
Chantal
C
Ide
Christine
C
Fortin
Marc G
MG
Laliberté
Jean-François
JF
D017360:Arabidopsis / Q000235:genetics / Q000502:physiology / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D054884:Host-Pathogen Interactions; D020648:Peptide Elongation Factor 1 / Q000235:genetics / Q000502:physiology*; D010447:Peptide Hydrolases / Q000235:genetics / Q000502:physiology*; D030821:Plants, Genetically Modified; D017800:Potyvirus / Q000472:pathogenicity / Q000502:physiology*; D011485:Protein Binding; D012324:RNA-Dependent RNA Polymerase / Q000502:physiology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D014026:Nicotiana / Q000235:genetics / Q000502:physiology; D014779:Virus Replication
D029681:Arabidopsis Proteins; D017931:DNA Primers; D020648:Peptide Elongation Factor 1; D011993:Recombinant Fusion Proteins; D012324:RNA-Dependent RNA Polymerase; D010447:Peptide Hydrolases