title: Antioxidant properties of grape seed extract on human lymphocyte oxidative defence.
abstract: The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and (13)C-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catechin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 microg/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 microg/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 microg/mL increased catalase and glutathione S-transferase activity by 10% and 15%, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS.
authors.affiliation: Institute of Nuclear Science Vinca, University of Belgrade, Belgrade, Serbia.
authors.forename: Miroslava
authors.initials: M
authors.lastname: Stanković
authors.forename: Vele
authors.initials: V
authors.lastname: Tesević
authors.forename: Vlatka
authors.initials: V
authors.lastname: Vajs
authors.forename: Nina
authors.initials: N
authors.lastname: Todorović
authors.forename: Slobodan
authors.initials: S
authors.lastname: Milosavljević
authors.forename: Dejan
authors.initials: D
authors.lastname: Godevac
mesh_terms: D000975:Antioxidants / Q000032:analysis / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D002478:Cells, Cultured; D005982:Glutathione Transferase / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D015162:Micronucleus Tests; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry; D027843:Vitis / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D008315:Malondialdehyde; D002374:Catalase; D005982:Glutathione Transferase