[Serum proteomic variation study in patients with Crohn disease].
OBJECTIVE
To search differentially expressed proteins in serum of patients with Crohn disease.


METHODS
Serum protein samples from 4 patients with Crohn disease and 8 healthy adults were recruited cross-labeled with variant CyDye, and then followed by two-dimensional differential in-gel electrophoresis (2-D DIGE), image analysis, and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).


RESULTS
The 2-D electrophoresis results were compared between the Crohn disease patients and the healthy adults. The spot 1058 expression in serum of Crohn disease patients increased by 1.68 folds as compared with healthy adults (P<0.05). The protein was identified as haptoglobin by mass spectrometry.


CONCLUSIONS
Up-regulating expression of haptoglobin in serum of Crohn disease patients may play a role in disequilibrium of immunity system.
Kang
Liang
L
Department of Colorectal Surgery, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou 510655, China.
Yang
Zu-Li
ZL
Liu
Wei
W
Zhang
Long-Juan
LJ
Liu
Shao-Jun
SJ
Huang
Mei-Jin
MJ
Li
Ming-Tao
MT
Wang
Jian-Ping
JP
D000328:Adult; D001798:Blood Proteins / Q000378:metabolism*; D016022:Case-Control Studies; D003424:Crohn Disease / Q000097:blood*; D015180:Electrophoresis, Gel, Two-Dimensional; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001798:Blood Proteins; D006242:Haptoglobins