title: Faf1 is expressed during neurodevelopment and is involved in Apaf1-dependent caspase-3 activation in proneural cells.
abstract: Fas-associated factor 1 (Faf1) has been described as a Fas-binding pro-apoptotic protein and as a component of the death-inducing signaling complex (DISC) in Fas-mediated apoptosis. Faf1 is able to potentiate Fas-induced apoptosis in several cell lines, although its specific functions are still not clear. Here we show that Faf1 is highly expressed in several areas of the developing telencephalon. Its expression pattern appears to be dynamic at different embryonic stages and to be progressively confined within limited territories. To decipher the specific role of Faf1 in developing brain, we used cDNA over-expression and mRNA down-regulation experiments to modulate Faf1 expression in telencephalic neural precursor cells, and we showed that in neural cell death Faf1 acts as a Fas-independent apoptotic enhancer. Moreover, we found that Faf1 protein level is down-regulated during apoptosis in a caspase- and Apaf1-dependent manner.
authors.affiliation: Laboratory of Molecular Neuroembryology, IRCCS Fondazione Santa Lucia, Rome, Italy.
authors.forename: D
authors.initials: D
authors.lastname: De Zio
authors.forename: E
authors.initials: E
authors.lastname: Ferraro
authors.forename: M
authors.initials: M
authors.lastname: D'Amelio
authors.forename: V
authors.initials: V
authors.lastname: Simoni
authors.forename: M
authors.initials: M
authors.lastname: Bordi
authors.forename: D
authors.initials: D
authors.lastname: Soroldoni
authors.forename: L
authors.initials: L
authors.lastname: Berghella
authors.forename: B I
authors.initials: BI
authors.lastname: Meyer
authors.forename: F
authors.initials: F
authors.lastname: Cecconi
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D017209:Apoptosis / Q000502:physiology; D051017:Apoptosis Regulatory Proteins; D053477:Apoptotic Protease-Activating Factor 1 / Q000235:genetics / Q000378:metabolism*; D001921:Brain / Q000033:anatomy & histology / Q000196:embryology* / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism*; D053148:Caspase 3 / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D004789:Enzyme Activation; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; D053477:Apoptotic Protease-Activating Factor 1; D002352:Carrier Proteins; C097186:Faf1 protein, mouse; D047908:Intracellular Signaling Peptides and Proteins; D011993:Recombinant Fusion Proteins; D053148:Caspase 3