Ultrasensitive colorimetric detection of protein by aptamer-Au nanoparticles conjugates based on a dot-blot assay.
A simple, rapid and ultrasensitive colorimetric detection of protein using aptamer-Au nanoparticles (AuNPs) conjugates based on a dot-blot array has been developed, which was combined with the unique optical properties of AuNPs, enabling the visual detection of protein within minutes without any instrument.
Wang
Yuling
Y
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, and Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, PR China.
Li
Dan
D
Ren
Wen
W
Liu
Zuojia
Z
Dong
Shaojun
S
Wang
Erkang
E
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D003101:Collodion / Q000737:chemistry; D003124:Colorimetry / Q000379:methods; D006046:Gold / Q000737:chemistry*; D006801:Humans; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D011506:Proteins / Q000032:analysis*; D015203:Reproducibility of Results; D012834:Silver / Q000737:chemistry; D013917:Thrombin / Q000032:analysis
D052157:Aptamers, Nucleotide; D008567:Membranes, Artificial; D011506:Proteins; D012834:Silver; D006046:Gold; D003101:Collodion; D013917:Thrombin