title: Effect of a papain-based gel for chemomechanical caries removal on dentin shear bond strength.
abstract: OBJECTIVE
  The aim of this study was to assess the shear bond strength of an adhesive restorative system on sound and demineralized dentin after the use of a papain-based agent.
  
  
  METHODS
  Forty human dentin slabs were randomly distributed into 4 groups: (1) sound dentin slabs that received an application of papain-based gel (N=10); (2) sound dentin slabs that did not receive a papain-based gel application (N=10); (3) demineralized slabs that received an application of the agent (N=10); and (4) demineralized slabs that did not receive an application of the agent (N=10). After manual excavation and use of the chemomechanical agent, the slabs were restored with a total etch adhesive system and microhybrid resin composite. The test specimens were individually stored in a damp environment for 7 days, and the shear bond strength test was performed using a universal test machine at a speed of 0.5 mm/min. The fragments were observed under a stereoscopic microscope to assess the fracture mode.
  
  
  RESULTS
  Fracture mode assessment showed adhesive and cohesive type fractures in resin for all the groups. The analysis of variance and the Tukey test showed that there were no differences in the shear bond strength means among the groups (P>.05).
  
  
  CONCLUSIONS
  The use of a papain-based gel to remove dental caries did not interfere in the bond strength of restorative materials to dentin.
authors.affiliation: Araras Dental School, Hermínio Ometto University Center (UNIARARAS), Araras, São Paulo, Brazil.
authors.forename: Michelle Cristina
authors.initials: MC
authors.lastname: Lopes
authors.forename: Raphaella Capitoni
authors.initials: RC
authors.lastname: Mascarini
authors.forename: Bruna Maria Covre Garcia
authors.initials: BM
authors.lastname: da Silva
authors.forename: Flávia Martão
authors.initials: FM
authors.lastname: Flório
authors.forename: Roberta Tarkany
authors.initials: RT
authors.lastname: Basting
mesh_terms: D000134:Acid Etching, Dental; D000704:Analysis of Variance; D017438:Bisphenol A-Glycidyl Methacrylate; D003188:Composite Resins; D001840:Dental Bonding; D003737:Dental Cavity Preparation / Q000379:methods*; D003799:Dental Stress Analysis; D003804:Dentin / Q000187:drug effects; D003806:Dentin Permeability; D017220:Dentin-Bonding Agents; D005782:Gels; D006801:Humans; D008422:Materials Testing; D010206:Papain / Q000494:pharmacology*; D019279:Resin Cements; D033081:Shear Strength; D012822:Silicon Dioxide; D017001:Tooth Demineralization; D015040:Zirconium
chemical_list: D003188:Composite Resins; D017220:Dentin-Bonding Agents; D005782:Gels; C509557:Papacarie; D019279:Resin Cements; C083568:Z100 composite resin; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; D012822:Silicon Dioxide; D015040:Zirconium; D010206:Papain