[Monitoring of antibiotic resistance of gram negative anaerobes].
METHODS
Using an agar reference method (Norma M11-A5, National Committee for Clinical and Laboratory Standards) the minimal inhibitory concentrations of nine antibiotics were determined for 376 anaerobic strains. The following strains were investigated: 254 Bacteroides fragilis group (including 143 B. fragilis), 122 other gram-negative anaerobes (Bacteroides spp., Prevotella, Fusobacterium, Porphyromonas, Suterella, Desulfomonas, Veillonella).


RESULTS
In the B. fragilis group resistance rates were: coamoxyclav 2.8%, ticarcillin 27.5%, ticarcillin-clavulanic acid 1.9%, piperacillin-tazobactam 1.9%, cefoxitin 6.2%, imipenem 0.8%, clindamycin 28.3%, respectively. Based on previous studies, resistance to imipenem remained low in 2003 and was only observed for B. fragilis. Resistance to clindamycin was maintained around 25%. No metronidazole resistance was observed, but decreased susceptibility was found for B. fragilis, B. merdae and Prevotella, as in 4.3% of gram-negative anaerobes.


CONCLUSIONS
This study confirms the high resistance rate of gram-negative anaerobes to clindamycin, the efficient activity of imipenem, beta-lactam/beta-lactamase inhibitor combinations and metronidazole. However, reduced metronidazole susceptibility seems to be increasing.
Singer
E
E
Laboratoire de bactériologie clinique, faculté des sciences pharmaceutiques et biologiques, 3, rue du Professeur-Laguesse, BP 83, 59006 Lille cedex, France.
Calvet
L
L
Mory
F
F
Muller
C
C
Chomarat
M
M
Bézian
M-C
MC
Bland
S
S
Juvenin
M-E
ME
Drugeon
H
H
Fosse
T
T
Goldstein
F
F
Jaulhac
B
B
Monteil
H
H
Marchandin
H
H
Jean-Pierre
H
H
Dubreuil
L
L
D000005:Abdomen / Q000382:microbiology; D000900:Anti-Bacterial Agents / Q000145:classification / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid / Q000382:microbiology; D004352:Drug Resistance, Microbial / Q000502:physiology*; D016946:Gram-Negative Aerobic Rods and Cocci / Q000187:drug effects* / Q000302:isolation & purification; D006801:Humans; D012867:Skin / Q000382:microbiology
D000900:Anti-Bacterial Agents