title: Effect of fungal strain and cereal substrate on in vitro mycotoxin production by Fusarium poae and Fusarium avenaceum.
abstract: Single conidia strains of Fusarium poae and Fusarium avenaceum were investigated for their ability to synthesise mycotoxins in vitro. In a first experiment, rice was inoculated with three strains of F. poae and three strains of F. avenaceum. In a second experiment, the same strains were cultivated on four different cereal substrates. For both experiments, the colonised substrates were analysed using liquid chromatography coupled with tandem mass spectrometric detection (LC-MS/MS) for the content of Fusarium mycotoxins. On rice, a strong effect of strains on mycotoxin content was found. With the analyses of different cereal types, substantial substrate effects were observed. For F. poae, these effects were highly dependent on the strain. The results of this study are of benefit for risk assessment concerning naturally infected grain samples since substrate effects could also be triggered by different cereal species or varieties, and thus could lead to variable mycotoxin contamination in cereal products intended for human consumption.
authors.affiliation: Research Station Agroscope Reckenholz-Tänikon ART, Zurich, Switzerland. susanne.vogelgsang@art.admin.ch
authors.forename: Susanne
authors.initials: S
authors.lastname: Vogelgsang
authors.forename: Michael
authors.initials: M
authors.lastname: Sulyok
authors.forename: Irene
authors.initials: I
authors.lastname: Bänziger
authors.forename: Rudolf
authors.initials: R
authors.lastname: Krska
authors.forename: Rainer
authors.initials: R
authors.lastname: Schuhmacher
authors.forename: Hans-Rudolf
authors.initials: HR
authors.lastname: Forrer
mesh_terms: D002523:Edible Grain / Q000382:microbiology*; D005504:Food Analysis / Q000379:methods; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D005670:Fusarium / Q000145:classification / Q000378:metabolism* / Q000472:pathogenicity; D009183:Mycotoxins / Q000096:biosynthesis*; D012275:Oryza / Q000382:microbiology; D013045:Species Specificity
chemical_list: D009183:Mycotoxins