title: Molecular and phenotypic comparison of phaeochromycin-producing strains of Streptomyces phaeochromogenes and Streptomyces ederensis.
abstract: Streptomyces strain LL-P018 produces the phaeochromycins, novel anti-inflammatory polyketides. This organism was identified as a strain of Streptomyces phaeochromogenes by physiological and genetic taxonomic analysis. In order to gain greater taxonomic perspective, LL-P018 was compared to related strains from major culture collections by 16S rRNA gene sequence, ribotype, HPLC-MS metabolite profile, and rpoB sequence. Using BioNumerics software, genetic and chemical fingerprint data were integrated via multivariate cluster analysis into a single, robust comparison. Based upon this analysis, strain LL-P018 is very closely related to the type strains of both S. phaeochromogenes and Streptomyces ederensis, indicating that these two types may in fact represent a single species. This novel comparative multi-cluster analysis is most useful for clarifying relationships between closely related species.
authors.affiliation: Wyeth Research, Pearl River, NY, USA. ritaccf@wyeth.com
authors.forename: Frank V
authors.initials: FV
authors.lastname: Ritacco
authors.forename: Douglas E
authors.initials: DE
authors.lastname: Eveleigh
mesh_terms: D054378:Acetogenins / Q000737:chemistry / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics; D001483:Base Sequence; D050260:Carbohydrate Metabolism; D002851:Chromatography, High Pressure Liquid; D016000:Cluster Analysis; D003470:Culture Media; D008969:Molecular Sequence Data; D010641:Phenotype; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D021521:Ribotyping; D016415:Sequence Alignment; D013045:Species Specificity; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D054378:Acetogenins; D001426:Bacterial Proteins; D003470:Culture Media; D012336:RNA, Ribosomal, 16S