title: Analysis of monthly isolation of respiratory viruses from children by cell culture using a microplate method: a two-year study from 2004 to 2005 in yamagata, Japan.
abstract: Although well over 200 viral agents have been implicated in acute respiratory infections (ARIs) among children, no system able to detect such a wide range of viruses has been established. Between January 2004 and December 2005, a modified microplate method, including HEF, HEp-2, Vero E6, MDCK, RD-18S, and GMK cell lines (HHVe6MRG plate), was adopted to isolate viruses. A total of 1,551 viruses were isolated, representing both outbreaks and sporadic cases, from 4,107 nasopharyngeal specimens, at monthly isolation rates of 22.3 to 52.6%. Influenza, parainfluenza, respiratory syncytial (RS), and mumps viruses, and human metapneumovirus, enterovirus, parechovirus, rhinovirus, adenovirus, herpesvirus, and cytomegalovirus were all isolated. The use of multiple cell lines increased the isolation rates of most of these viruses. The findings showed that ARIs due to a number of respiratory viruses occurred across all seasons in succession and/or concurrently in children in the community. These data will help clinicians determine in which seasons and for which age groups they should use the rapid diagnostic test kits available for influenza virus, RS virus, and adenovirus. In conclusion, we verified that the modified microplate method was able to clarify the etiology and epidemiology of numerous viruses isolated from children with ARI.
authors.affiliation: Department of Microbiology, Yamagata Prefectural Institute of Public Health, Yamagata 990-0031, Japan. mizutak@pref.yamagata.jp
authors.forename: Katsumi
authors.initials: K
authors.lastname: Mizuta
authors.forename: Chieko
authors.initials: C
authors.lastname: Abiko
authors.forename: Yoko
authors.initials: Y
authors.lastname: Aoki
authors.forename: Asuka
authors.initials: A
authors.lastname: Suto
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Hoshina
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Itagaki
authors.forename: Noriko
authors.initials: N
authors.lastname: Katsushima
authors.forename: Yoko
authors.initials: Y
authors.lastname: Matsuzaki
authors.forename: Seiji
authors.initials: S
authors.lastname: Hongo
authors.forename: Masahiro
authors.initials: M
authors.lastname: Noda
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Kimura
authors.forename: Katsumi
authors.initials: K
authors.lastname: Ootani
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000818:Animals; D002460:Cell Line; D002648:Child; D002675:Child, Preschool; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007564:Japan / Q000453:epidemiology; D012141:Respiratory Tract Infections / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology*; D012621:Seasons; D014776:Virus Cultivation / Q000295:instrumentation / Q000379:methods; D014777:Virus Diseases / Q000175:diagnosis* / Q000453:epidemiology / Q000821:virology; D014780:Viruses / Q000145:classification / Q000302:isolation & purification*