Airway eosinophilic inflammation is attenuated in conserved noncoding sequence-1-deficient mice.
BACKGROUND
Conserved noncoding sequence-1 (CNS-1) is an important regulatory element for T helper 2 cytokine expression. IL-4, IL-5 and IL-13 expression as well as serum IgE level were attenuated in CNS-1-/- mice.


METHODS
CNS-1-/- and CNS-1+/+ mice were sensitized with ovalbumin (OVA) followed by antigen challenge. The number of eosinophils and T helper 2 cytokine concentration in the bronchoalveolar lavage fluid, OVA-specific IgE antibody (Ab) in the serum and bronchial responsiveness to methacholine were examined.


RESULTS
Bronchoalveolar lavage fluid eosinophilia was significantly attenuated in CNS-1-/- mice compared to CNS-1+/+ mice, which were sensitized with OVA/aluminum once. OVA-specific IgE Ab was also attenuated. When mice were sensitized with OVA/aluminum twice, induction of eosinophilia and OVA-specific IgE Ab was not significantly different between CNS-1-/- and CNS-1+/+ mice.


CONCLUSIONS
CNS-1 locus regulates eosinophilic inflammation in vivo.
Ohtomo
Takayuki
T
National Hospital Organization, Sagamihara National Hospital, Clinical Research Center for Allergy and Rheumatology, Sagamihara, Japan.
Miyatake
Shoichiro
S
Kajiyama
Yuichiro
Y
Umezu-Goto
Makiko
M
Kobayashi
Noriaki
N
Kaminuma
Osamu
O
Mori
Akio
A
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology* / Q000276:immunology*; D017124:Conserved Sequence / Q000235:genetics; D021901:DNA, Intergenic / Q000235:genetics*; D004802:Eosinophilia / Q000235:genetics / Q000503:physiopathology*; D004804:Eosinophils / Q000276:immunology*; D007073:Immunoglobulin E / Q000378:metabolism; D007074:Immunoglobulin G / Q000378:metabolism; D007249:Inflammation / Q000235:genetics*; D018793:Interleukin-13 / Q000235:genetics; D015847:Interleukin-4 / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D050436:Regulatory Elements, Transcriptional / Q000235:genetics*; D018418:Th2 Cells / Q000276:immunology
D021901:DNA, Intergenic; D007074:Immunoglobulin G; D018793:Interleukin-13; D015847:Interleukin-4; D007073:Immunoglobulin E