title: B-lineage commitment prior to surface expression of B220 and CD19 on hematopoietic progenitor cells.
abstract: Commitment of hematopoietic progenitor cells to B-lymphoid cell fate has been suggested to coincide with the development of PAX5-expressing B220(+)CD19(+) pro-B cells. We have used a transgenic reporter mouse, expressing human CD25 under the control of the B-lineage-restricted Igll1 (lambda5) promoter to investigate the lineage potential of early progenitor cells in the bone marrow. This strategy allowed us to identify a reporter expressing LIN(-)B220(-)CD19(-)CD127(+)FLT3(+)SCA1(low)KIT(low) population that displays a lack of myeloid and a 90% reduction in in vitro T-cell potential compared with its reporter-negative counterpart. Gene expression analysis demonstrated that these lineage-restricted cells express B-lineage-associated genes to levels comparable with that observed in pro-B cells. These data suggest that B-lineage commitment can occur before the expression of B220 and CD19.
authors.affiliation: Department for Biomedicine and Surgery, Linköping University, Linköping, Sweden.
authors.forename: Robert
authors.initials: R
authors.lastname: Mansson
authors.forename: Sasan
authors.initials: S
authors.lastname: Zandi
authors.forename: Kristina
authors.initials: K
authors.lastname: Anderson
authors.forename: Inga-Lill
authors.initials: IL
authors.lastname: Martensson
authors.forename: Sten Eirik W
authors.initials: SE
authors.lastname: Jacobsen
authors.forename: David
authors.initials: D
authors.lastname: Bryder
authors.forename: Mikael
authors.initials: M
authors.lastname: Sigvardsson
mesh_terms: D000818:Animals; D018941:Antigens, CD19 / Q000032:analysis*; D001402:B-Lymphocytes / Q000737:chemistry / Q000166:cytology*; D001854:Bone Marrow Cells; D019070:Cell Lineage; D006412:Hematopoietic Stem Cells / Q000737:chemistry / Q000166:cytology*; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit; D017493:Leukocyte Common Antigens / Q000032:analysis*; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
chemical_list: D018941:Antigens, CD19; D053645:Interleukin-2 Receptor alpha Subunit; D017493:Leukocyte Common Antigens