[Search for chaperon-like anticataract drugs, the antiaggregants of lens crystallins. Communication. 1. Chaperon-like activity of N-acetyl carnosine dipeptide: in vitro study on a model of ultraviolet-induced aggregation of betaL-crystallin].
Aggregation ofcrystallins, the lens proteins, is one of the basic stages of cataract formation. Among the protein aggregation models used to study the molecular mechanisms of the initial stages of lenticular opacity, UV-induced aggregation of betaL-crystallin is most close to the in vivo conditions. The carnosine derivative N-acetyl carnosine has been shown to be effective in inhibiting the UV-induced aggregation of betaL-crystallin. Examination of the accumulation kinetics of carbonyl groups in betaL-crystallin under UV irradiation has indicated that neither carnosine nor N-acetyl carnosine fails to affect this parameter--an indicator of oxidative protein damage. By taking into account also the fact that N-acetyl carnosine is not an antioxidant, it can be believed that the molecular mechanism of action of this compound on UV-induced aggregation of betaL is unassociated with its antioxidative properties. The authors hypothesize that the molecular chaperon-like properties similar to those of alpha-crystallin underlie the mechanism of action of the acetyl derivative carnosine. The prospects for searching anticataract agents of a new chaperon-like class are discussed.
Muranov
K O
KO
Dizhevskaia
A K
AK
Boldyrev
A A
AA
Karpova
O E
OE
Sheremet
N L
NL
Polunin
G S
GS
Avetisov
S E
SE
Ostrovskiĭ
M A
MA
D000818:Animals; D002336:Carnosine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D002386:Cataract / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D002417:Cattle; D004195:Disease Models, Animal; D018450:Disease Progression; D007908:Lens, Crystalline / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D008297:Male; D018832:Molecular Chaperones / Q000627:therapeutic use*; D014466:Ultraviolet Rays / Q000009:adverse effects; D038204:beta-Crystallins / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects
D018832:Molecular Chaperones; D038204:beta-Crystallins; C033681:N-acetylcarnosine; D002336:Carnosine