Structural and functional properties of amylose complexes with genistein.
Complexes of amylose or high-amylose corn starch (HACS) with genistein were prepared by the acidification of an alkali solution to yield a V 6IotaIotaIota structure. The amylose-genistein complexes exhibited significantly higher genistein content (11 mg/100 mg of complex) than HACS-genistein complexes (9 mg/100 mg of complex). The effect of genistein on the amylose complexes was examined in different genistein-amylose ratios, and a model for genistein organization in the amylose complexes was suggested. The complexes were stable at different pH values, with <10% of the complexed genistein released, and were stable at 30 and 50 degrees C. Lower stability was observed at 80 degrees C as shown by the extensive release of genistein. All complexes showed high retention of genistein in simulated stomach conditions and released genistein upon digestion in pancreatin solution. It is therefore suggested that the complexes can be used as carriers for the slow release of genistein.
Cohen
Revital
R
Faculty of Biotechnology and Food Engineering, Technion--Israel Institute of Technology, Haifa, Israel.
Orlova
Yevgenia
Y
Kovalev
Marina
M
Ungar
Yael
Y
Shimoni
Eyal
E
D000688:Amylose / Q000032:analysis / Q000737:chemistry*; D004063:Digestion; D004355:Drug Stability; D019833:Genistein / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010194:Pancreatin / Q000378:metabolism; D010316:Particle Size; D012996:Solutions; D013329:Structure-Activity Relationship; D014961:X-Ray Diffraction
D012996:Solutions; D010194:Pancreatin; D000688:Amylose; D019833:Genistein