[Effects of vitamin C combined with arsenic trioxide on the apoptosis of Hep-2 cell].
OBJECTIVE
To study synergistic effect of Vitamin C (VitC) combined with arsenic trioxide (As2 O3)-on human laryngocarcinoma Hep-2 cell line apoptosis.


METHODS
Human laryngocarcinoma cell line Hep-2 was cultured in vitro, and incubated with As2 O3 or jointly with VitC. The inhibition ability of Hep-2 cells was determined by methyl thiazolyl tetrazolium (MTT) assay, while the apoptosis was mensurated by the flow cytometry with Annexin-V/PI.


RESULTS
Compared with the use of As2 O3 separately, the combination of As2 O3 and VitC could increase the inhibition ability and the apoptosis rate of Hep-2 cells markedly (P < 0.05).


CONCLUSIONS
Combination of As2 O3 with VitC could markedly increase arsenic trioxide (As2 O3)-induced apoptosis of Hep-2 cells.
Xu
Weimin
W
Department of Otolaryngology, Renmin Hospital of Tongshan County, 437600, China.
Shu
Chenghua
C
Hu
Jin
J
Ye
Yuhua
Y
D017209:Apoptosis / Q000187:drug effects*; D000077237:Arsenic Trioxide; D001152:Arsenicals / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology*; D045744:Cell Line, Tumor; D004357:Drug Synergism; D006801:Humans; D010087:Oxides / Q000494:pharmacology*
D001152:Arsenicals; D010087:Oxides; D001205:Ascorbic Acid; D000077237:Arsenic Trioxide