On the functional role of a water molecule in clade 3 catalases: a proposal for the mechanism by which NADPH prevents the formation of compound II.
X-ray structures of the 13 different monofunctional heme catalases published to date were scrutinized in order to gain insight in the mechanism by which NADPH in Clade 3 catalases may protect the reactive ferryloxo intermediate Compound I (Cpd I; por (*+)Fe (IV)O) against deactivation to the catalytically inactive intermediate Compound II (Cpd II; porFe (IV)O). Striking similarities in the molecular network of the protein subunits encompassing the heme center and the surface-bound NADPH were found for all of the Clade 3 catalases. Unique features in this region are the presence of a water molecule (W1) adjacent to the 4-vinyl group of heme and a serine residue or a second water molecule hydrogen-bonded to both W1 and the carbonyl group of a threonine-proline linkage, with the proline in van der Waals contact with the dihydronicotinamide group of NADPH. A mechanism is proposed in which a hydroxyl anion released from W1 undergoes reversible nucleophilic addition to the terminal carbon of the 4-vinyl group of Cpd I, thereby producing a neutral porphyrin pi-radical ferryloxo (HO-por (*)Fe (IV)O) species of reduced reactivity. This structure is suggested to be the elusive Cpd II' intermediate proposed in previous studies. An accompanying proton-shifting process along the hydrogen-bonded network is believed to facilitate the NADPH-mediated reduction of Cpd I to ferricatalase and to serve as a funnel for electron transfer from NADPH to the heme center to restore the catalase Fe (III) resting state. The proposed reaction paths were fully supported as chemically reasonable and energetically feasible by means of density functional theory calculations at the (U)B3LYP/6-31G* level. A particularly attractive feature of the present mechanism is that the previously discussed formation of protein-derived radicals is avoided.
Sicking
Willi
W
Institut für Organische Chemie, Universität Duisburg-Essen, 45117 Essen, Germany.
Korth
Hans-Gert
HG
de Groot
Herbert
H
Sustmann
Reiner
R
D002374:Catalase / Q000737:chemistry* / Q000378:metabolism*; D018360:Crystallography, X-Ray; D005290:Ferric Compounds / Q000737:chemistry*; D006418:Heme / Q000737:chemistry / Q000378:metabolism; D008958:Models, Molecular; D009249:NADP / Q000737:chemistry* / Q000378:metabolism*; D011789:Quantum Theory; D013329:Structure-Activity Relationship; D013816:Thermodynamics
D005290:Ferric Compounds; D006418:Heme; D009249:NADP; D002374:Catalase