Neuropeptide Y promotes TGF-beta1 production in RAW264.7 cells by activating PI3K pathway via Y1 receptor.
OBJECTIVE
To examine the effect of neuropeptide Y (NPY) on TGF-beta1 production in RAW264.7 macrophages.


METHODS
Enzyme linked immunosorbent assay (ELISA) was used to detect TGF-beta1 production. Cell counting kit 8 (CCK-8) was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85.


RESULTS
NPY treatment could promote TGF-beta1 production and rapid phosphorylation of PI3K p85 in RAW264.7 cells via Y1 receptor. The elevated TGF-beta1 production induced by NPY could be abolished by wortmannin pretreatment.


CONCLUSIONS
NPY may elicit TGF-beta1 production in RAW264.7 cells via Y1 receptor, and the activated PI3K pathway may account for this effect.
Zhou
Jiang-Rui
JR
Department of Nautical Medicine, Laboratory of Stress Medicine, Second Military Medical University, Shanghai, China.
Xu
Zheng
Z
Jiang
Chun-Lei
CL
D000730:Androstadienes / Q000494:pharmacology; D000818:Animals; D015153:Blotting, Western; D002452:Cell Count; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D004789:Enzyme Activation / Q000187:drug effects / Q000502:physiology; D004797:Enzyme-Linked Immunosorbent Assay; D007166:Immunosuppressive Agents / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000378:metabolism*; D051379:Mice; D009478:Neuropeptide Y / Q000378:metabolism* / Q000494:pharmacology; D019869:Phosphatidylinositol 3-Kinases / Q000187:drug effects / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects; D017476:Receptors, Neuropeptide Y / Q000819:agonists / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects / Q000276:immunology*; D053773:Transforming Growth Factor beta1 / Q000819:agonists / Q000378:metabolism*; D015854:Up-Regulation / Q000187:drug effects / Q000276:immunology; D000077191:Wortmannin
D000730:Androstadienes; D007166:Immunosuppressive Agents; D009478:Neuropeptide Y; C507698:Npy1r protein, mouse; D017476:Receptors, Neuropeptide Y; D053773:Transforming Growth Factor beta1; D019869:Phosphatidylinositol 3-Kinases; D000077191:Wortmannin