title: Purification and biochemical characterization of two xylanases from Aspergillus sydowii SBS 45.
abstract: Two xylanases were isolated and purified from crude culture filtrate of Aspergillus sydowii SBS 45 after 9 days of growth on wheat bran containing 0.5% (w/v) birch wood xylan as the carbon source under solid-state fermentation. After a three-step purification scheme involving ammonium sulfate precipitation, gel filtration chromatography (Sephadex G-200), and anion exchange chromatography (DEAE-Sephadex A-50), xylanase I was purified 93.41 times, and xylanase II was purified 77.40 times with yields of 4.49 and 10.46, respectively. Molecular weights of xylanase I and II were 20.1 and 43 kDa, respectively, in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Optimum temperature was 50 degrees C, and optimum pH was 10.0 for both xylanase I and II. The Km value of xylanase I for birch wood xylan was 3.18 mg ml(-1) and for oat spelt xylan 6.45 mg ml(-1), while the Km value of xylanase II for birch wood xylan was 6.51 mg ml(-1) and for oat spelt xylan 7.69 mg ml(-1). Metal ions like Al3+, Ba2+, Ca2+, Na+, and Zn2+ enhanced the activity of xylanase I and II at 10 mM concentration. Among the additives, L-tryptophan enhanced the activity of xylanase I and II at 10-, 20-, and 30-mM concentrations. Both xylanases appeared to be glycoproteins.
authors.affiliation: School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India. suprabha.nair@gmail.com
authors.forename: Suprabha G
authors.initials: SG
authors.lastname: Nair
authors.forename: R
authors.initials: R
authors.lastname: Sindhu
authors.forename: Shankar
authors.initials: S
authors.lastname: Shashidhar
mesh_terms: D001230:Aspergillus / Q000201:enzymology*; D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D007700:Kinetics; D013379:Substrate Specificity; D013696:Temperature; D014995:Xylosidases / Q000302:isolation & purification* / Q000378:metabolism
chemical_list: D014995:Xylosidases