Concordant over-expression of transcription factor Sp1 and vascular endothelial growth factor in extramammary Paget's disease.
BACKGROUND
Specificity protein 1 (Sp1) is a transcription factor and shown to be a sequence-specific DNA-binding protein that activate a broad and diverse spectrum of mammalian gene, such as vascular endothelial growth factor (VEGF) gene. But the expression of Sp1 and VEGF has not previously been investigated in extramammary Paget's disease (EMPD).


METHODS
To investigate the expression of Sp1 and VEGF proteins in EMPD and to assess their relationships and potential contribution to malignant transduction of EMPD, paraffin-embedded EMPD specimens (35 tissue samples from 33 patients with primary EMPD, including two samples of metastatic lymph nodes from two patients) were subjected to immunohistochemical staining for Sp1 and VEGF.


RESULTS
All of the 35 EMPD specimens, including all of six invasive EMPD and two metastatic lymph node specimens, showed strong nuclear positive staining for Sp1 and strong cytoplasmic positive staining for VEGF. The expression levels (% positive cells) of Sp1 and VEGF in EMPD were significantly higher than those of normal skin (NS). There was a significantly high correlation between expression levels of Sp1 and VEGF in EMPD.


CONCLUSIONS
The present study reveals that the concordant over-expression of Sp1 and VEGF may play a pivotal role in the tumorigenesis and further malignant transduction of EMPD.
Chen
Si-Yuan
SY
Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Takeuchi
Satoshi
S
Moroi
Yoichi
Y
Hayashida
Sayaka
S
Kido
Makiko
M
Uchi
Hiroshi
H
Takahara
Masakazu
M
Uenotsuchi
Takeshi
T
Tu
Ya-Ting
YT
Urabe
Kazunori
K
Furue
Masutaka
M
D000368:Aged; D000369:Aged, 80 and over; D001005:Anus Neoplasms / Q000378:metabolism / Q000473:pathology; D001365:Axilla / Q000473:pathology; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000378:metabolism*; D010145:Paget Disease, Extramammary / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D012867:Skin / Q000378:metabolism / Q000473:pathology; D012878:Skin Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D016329:Sp1 Transcription Factor / Q000378:metabolism*; D014565:Urogenital Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000556:secondary; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
D009363:Neoplasm Proteins; D016329:Sp1 Transcription Factor; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A