[Effect of human semaphorin-3F gene transient transfection on the proliferation of tongue carcinoma cells].
OBJECTIVE
To investigate the effect of semaphorin-3F (SEMA-3F) gene transient transfection on the proliferation of Tca8113 tongue carcinoma cells.


METHODS
After construction of a full-length SEMA3F expression vector, Tca8113 cells were transient transfected with pEGFP-N1-SEMA3F by Lipofectamine 2000. The expression of SEMA-3F gene was detected by RT-PCR The differences of the expression in the transfected cell groups, empty vector groups and un-transfected cell groups were compared. The survival rates were assayed by methyl thiazolyl tetrazolium (MTT) enzymatic labeling technique. Cell cycle were assayed by flow cytometer (FCM).


RESULTS
The gene was transfected into Tca8113 cells. High expression of SEMA-3F was successfully detected in the transfected cell groups after gene transfection. The cell cycle percentage of G1 stage decreased and S stage increased.


CONCLUSIONS
SEMA-3F gene transient transfection may inhibit the proliferation of Tca8113 cells.
Zhang
Jia-jia
JJ
Department of Oral and Maxillofacial Surgery, Stomatological College of Nanjing Medical University , Nanjing 210029, China.
Wu
Yu-nong
YN
Ye
Jin-hai
JH
Song
Xiao-meng
XM
D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005822:Genetic Vectors; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D014062:Tongue Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014162:Transfection
D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C491230:SEMA3F protein, human