title: Efficacious recombinant influenza vaccines produced by high yield bacterial expression: a solution to global pandemic and seasonal needs.
abstract: It is known that physical linkage of TLR ligands and vaccine antigens significantly enhances the immunopotency of the linked antigens. We have used this approach to generate novel influenza vaccines that fuse the globular head domain of the protective hemagglutinin (HA) antigen with the potent TLR5 ligand, flagellin. These fusion proteins are efficiently expressed in standard E. coli fermentation systems and the HA moiety can be faithfully refolded to take on the native conformation of the globular head. In mouse models of influenza infection, the vaccines elicit robust antibody responses that mitigate disease and protect mice from lethal challenge. These immunologically potent vaccines can be efficiently manufactured to support pandemic response, pre-pandemic and seasonal vaccines.
authors.affiliation: VaxInnate Corporation, Cranbury, New Jersey, United States of America. langzhou.song@vaxinnate.com
authors.forename: Langzhou
authors.initials: L
authors.lastname: Song
authors.forename: Valerian
authors.initials: V
authors.lastname: Nakaar
authors.forename: Uma
authors.initials: U
authors.lastname: Kavita
authors.forename: Albert
authors.initials: A
authors.lastname: Price
authors.forename: Jim
authors.initials: J
authors.lastname: Huleatt
authors.forename: Jie
authors.initials: J
authors.lastname: Tang
authors.forename: Andrea
authors.initials: A
authors.lastname: Jacobs
authors.forename: Ge
authors.initials: G
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Huang
authors.forename: Priyanka
authors.initials: P
authors.lastname: Desai
authors.forename: Gail
authors.initials: G
authors.lastname: Maksymiuk
authors.forename: Virginia
authors.initials: V
authors.lastname: Takahashi
authors.forename: Scott
authors.initials: S
authors.lastname: Umlauf
authors.forename: Lucia
authors.initials: L
authors.lastname: Reiserova
authors.forename: Rodney
authors.initials: R
authors.lastname: Bell
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.forename: William F
authors.initials: WF
authors.lastname: McDonald
authors.forename: T J
authors.initials: TJ
authors.lastname: Powell
authors.forename: Lynda
authors.initials: L
authors.lastname: Tussey
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000737:chemistry / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D009980:Influenza A virus / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D051379:Mice; D009500:Neutralization Tests; D011487:Protein Conformation; D012621:Seasons; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology
chemical_list: D000914:Antibodies, Viral; D000939:Epitopes; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic