Determination of total sterols in brown algae by Fourier transform infrared spectroscopy.
A procedure is proposed for the determination of the total amount of sterols in brown algae Bifurcaria bifurcata, Cladostephus hirsitus, Dictyota dichotoma and Cystoseira sedoides, globally determined as fucosterol, which is the major sterol contained in these algae. The method involves the use of cholesterol as reference standard and a correction factor of 1.259+/-0.003, which represents the ratio between the slopes of calibration lines obtained from fucosterol and cholesterol. The method provides precise and accurate results for the IR analysis of real samples.
Bouzidi
Naïma
N
Laboratory of Medicinal and Aromatic Plants, University of Blida, P.O.B. 270 Soumâa (Blida 09000), Algeria.
Daghbouche
Yasmina
Y
El Hattab
Mohamed
M
Aliche
Zahia
Z
Culioli
Gérald
G
Piovetti
Louis
L
Garrigues
Salvador
S
de la Guardia
Miguel
M
D000459:Phaeophyceae / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared / Q000295:instrumentation / Q000379:methods; D013261:Sterols / Q000032:analysis*
D013261:Sterols