Oxidative stress in leucocytospermic prostatitis patients: preliminary results.
The aim of this study was to contribute to the knowledge concerning pathogenesis of inflammatory chronic prostatitis by revealing possible shifts in the balance of markers of oxidative stress and anti-oxidative activity in case of leucocytospermic prostatitis. We also attempted to identify possible relations between seminal micro-organisms and oxidative stress parameters. A many-sided complex of local (spermatozoa, seminal plasma) and general (blood, urine) markers in 21 prostatitis patients and nine controls was compared. In both spermatozoa and seminal plasma, the content of diene conjugates was significantly higher in prostatitis patients compared with healthy controls. At the same time total anti-oxidative status in spermatozoa and total anti-oxidative activity in seminal plasma were lower in prostatitis patients than in controls. In urine, the level of 8-isoprostanes was significantly higher in prostatitis patients than in healthy controls, correlating well with 8-hydroxy-2'-deoxyguanosine. The latter correlated with cellular Fe and Ni contents as well, confirming that these metals with varying valency may cause DNA damage. Reduced glutathione showed higher levels in blood of controls than in prostatitis patients. Coryneform bacteria appeared to be associated with prostatitis-related oxidative stress. In conclusion, leucocytospermic prostatitis patients are characterised by oxidative stress at all levels: systemic (general), seminal plasma and cellular.
Kullisaar
T
T
Department of Biochemistry, University of Tartu, Tartu, Estonia.
Türk
S
S
Punab
M
M
Korrovits
P
P
Kisand
K
K
Rehema
A
A
Zilmer
K
K
Zilmer
M
M
Mändar
R
R
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D001205:Ascorbic Acid / Q000378:metabolism; D016022:Case-Control Studies; D002908:Chronic Disease; D003352:Corynebacterium / Q000145:classification / Q000302:isolation & purification; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D007962:Leukocytes / Q000378:metabolism; D008297:Male; D008670:Metals / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011446:Prospective Studies; D011472:Prostatitis / Q000097:blood / Q000209:etiology / Q000378:metabolism* / Q000382:microbiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D012661:Semen / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D013094:Spermatozoa / Q000378:metabolism*
C508600:IL6 protein, human; D015850:Interleukin-6; D008670:Metals; D017382:Reactive Oxygen Species; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine; D005978:Glutathione; D001205:Ascorbic Acid