[Proliferation cycle changes of Epstein-Barr virus in nasopharyngeal carcinoma cell line CNE after infection].
OBJECTIVE
The strategy of gene therapy on nasopharyngeal carcinoma (NPC) is determined by the characteristics of proliferation cycle of Epstein-Barr virus (EBV) in different histological types of NPC cells. This study was to investigate the characteristics of proliferation cycle of EBV in NPC cell line CNE2.


METHODS
CNE2 cells were co-cultivated with marmoset lymphocyte line B95-8, which was EBV-positive. Then B95-8 cells were removed by cytotoxic test of complement activation. The initial infection state of EBV in CNE2 cells was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) and Southern hybridization.


RESULTS
CNE2 cells were infected with EBV by cell-to-cell contacting. The transcription of BZLF1, the symbol gene for EBV lytic phase, maintained 12 days on average. The transcription of EBV-encoded small RNA (EBER), the symbol gene for EBV infectious phase, maintained 22 days on average.


CONCLUSIONS
After invading CNE2 cells, most EBV initially might be at lytic phase, then switch to the relatively stable latent phase, and finally be lost due to cell culture and passage.
He
Yu-Wen
YW
Center of Microbiology, Biochemistry and Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, Guangdong, 510080, People's Republic of China.
Zeng
Jun
J
Ou
Xue-Ling
XL
Zhang
Ge
G
Du
Jun
J
D000818:Animals; D001402:B-Lymphocytes / Q000821:virology*; D002144:Callithrix; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018920:Coculture Techniques; D004854:Herpesvirus 4, Human / Q000302:isolation & purification / Q000502:physiology; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000378:metabolism / Q000473:pathology* / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic; D017735:Virus Latency / Q000235:genetics
C063676:BZLF1 protein, Herpesvirus 4, Human; C099537:Epstein-Barr virus encoded RNA 1; C106732:Epstein-Barr virus encoded RNA 2; D012367:RNA, Viral; D015534:Trans-Activators