title: A bone-like precoating strategy for implants: collagen immobilization and mineralization on pure titanium implant surface.
abstract: Many surface modification strategies are currently of interest in improving integration of implants to bone. An in vitro precoating of a bone-like mineralized layer of immobilized collagen on the implant surface is a potentially valuable approach to improve host acceptance of the implant. The goal of this investigation was to develop a method to precoat in vitro a bone-like mineralized collagen layer on a pure titanium dental implant surface. The study was conducted on acid-etched and nonetched surfaces of screw implants. Initially, a procedure was standardized to self-assemble collagen from a collagen solution. In subsequent experiments, the implant was also placed inside the solution, and after 3 days, collagen was found to be coated on the implant surface. Mineralization of the collagen gel as well as collagen coating on the implant was carried out by calcium phosphate precipitation from a mineralizing solution of calcium chloride containing polyvinyl phosphonic acid and polyaspartic acid, which served as polyanionic additives to help disperse the precipitation and template mineral nucleation. The implant was kept in the mineralizing solution and maintained for 2 weeks in an incubator at 37 degrees C with a phosphate vapor phase generated from a vial containing dihydrogen ammonium phosphate in the incubator. Scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy analysis confirmed the coated layer to be a biomimetic bone-like mineralized type 1 collagen. Initial studies using osteoblast-like cells indicated cellular attachment on the modified surface. The method appears to be a promising way to generate in vitro a bone-like layer on the implant surface.
authors.affiliation: New Jersey Dental School, University of Medicine and Dentistry of New Jersey, 110 Bergen Street, Newark, NJ 07103, USA.
authors.forename: S
authors.initials: S
authors.lastname: Munisamy
authors.forename: T K
authors.initials: TK
authors.lastname: Vaidyanathan
authors.forename: J
authors.initials: J
authors.lastname: Vaidyanathan
mesh_terms: D040761:Biomimetic Materials; D002130:Calcium Phosphates; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible; D003094:Collagen / Q000737:chemistry; D045662:Dental Etching; D015921:Dental Implants; D017267:Dental Prosthesis Design; D004577:Electron Probe Microanalysis; D005782:Gels; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010006:Osteoblasts / Q000502:physiology; D012996:Solutions; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D014025:Titanium; D014961:X-Ray Diffraction
chemical_list: D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D015921:Dental Implants; D005782:Gels; D012996:Solutions; D003094:Collagen; C020243:calcium phosphate; D014025:Titanium