title: Polyvalent display of heme on hepatitis B virus capsid protein through coordination to hexahistidine tags.
abstract: The addition of a hexahistidine tag to the N terminus of the hepatitis B capsid protein gives rise to a self-assembled particle with 80 sites of high local density of histidine side chains. Iron protoporphyrin IX has been found to bind tightly at each of these sites, making a polyvalent system of well-defined spacing between metalloporphyrin complexes. The spectroscopic and redox properties of the resulting particle are consistent with the presence of 80 site-isolated bis(histidine)-bound heme centers, comprising a polyvalent b-type cytochrome mimic.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
authors.forename: Duane E
authors.initials: DE
authors.lastname: Prasuhn
authors.forename: Jane
authors.initials: J
authors.lastname: Kuzelka
authors.forename: Erica
authors.initials: E
authors.lastname: Strable
authors.forename: Andrew K
authors.initials: AK
authors.lastname: Udit
authors.forename: So-Hye
authors.initials: SH
authors.lastname: Cho
authors.forename: Gabriel C
authors.initials: GC
authors.lastname: Lander
authors.forename: Joel D
authors.initials: JD
authors.lastname: Quispe
authors.forename: James R
authors.initials: JR
authors.lastname: Diers
authors.forename: David F
authors.initials: DF
authors.lastname: Bocian
authors.forename: Clint
authors.initials: C
authors.lastname: Potter
authors.forename: Bridget
authors.initials: B
authors.lastname: Carragher
authors.forename: M G
authors.initials: MG
authors.lastname: Finn
mesh_terms: D001483:Base Sequence; D002213:Capsid / Q000737:chemistry / Q000378:metabolism*; D002850:Chromatography, Gel; D020285:Cryoelectron Microscopy; D017931:DNA Primers; D006418:Heme / Q000378:metabolism*; D006515:Hepatitis B virus / Q000378:metabolism*; D006639:Histidine / Q000737:chemistry / Q000378:metabolism*; D009842:Oligopeptides / Q000737:chemistry / Q000378:metabolism*; D013059:Spectrum Analysis, Raman
chemical_list: D017931:DNA Primers; C471213:His-His-His-His-His-His; D009842:Oligopeptides; D006418:Heme; D006639:Histidine