title: [Simultaneous determination of ochratoxin A, B and citrinin in foods by HPLC-FL and LC/MS/MS].
abstract: Methods using high-performance liquid chromatography with fluorescence detection (HPLC-FL) and using liquid chromatography with tandem mass spectrometry (LC/MS/MS) were developed for simultaneous determination of ochratoxin A (OTA), ochratoxin B (OTB) and citrinin (CIT) in cereal, fruit, and coffee products. The samples were extracted with ethyl acetate under an acidic condition, and then cleaned up with liquid-liquid separation. The test solutions were analyzed by reverse-phase HPLC-FL and LC/MS/MS. Mass spectral acquisition was performed in positive ion mode by applying multiple reaction monitoring. The performances of both detectors were almost equivalent. The recoveries of OTA and OTB were 87-111%, and that of CIT were 70-88%. The limits of quantification (S/N> or =10) of OTA, OTB and CIT was 0.1 mug/kg or less. These methods were considered to be useful for the determination of the three mycotoxins at low levels (0.1 microg/kg).
authors.affiliation: Tokyo Metropolitan Institute of Public Health, Tokyo, Japan.
authors.forename: Setsuko
authors.initials: S
authors.lastname: Tabata
authors.forename: Kenji
authors.initials: K
authors.lastname: Iida
authors.forename: Keisuke
authors.initials: K
authors.lastname: Kimura
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Iwasaki
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Nakazato
authors.forename: Kunihiro
authors.initials: K
authors.lastname: Kamata
authors.forename: Masako
authors.initials: M
authors.lastname: Hirokado
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D002953:Citrinin / Q000032:analysis*; D003069:Coffee / Q000737:chemistry; D002523:Edible Grain / Q000737:chemistry; D005504:Food Analysis / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005638:Fruit / Q000737:chemistry; D009793:Ochratoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D003069:Coffee; D009793:Ochratoxins; C025589:ochratoxin A; D002953:Citrinin; C045354:ochratoxin B