Antibody fragments may be incorrectly processed in the yeast Pichia pastoris.
We have studied the efficiency of N-terminal processing of the antibody light chain depending on the structure of the leader sequence when expressed in the yeast Pichia pastoris. The humanized light kappa-chain of the murine antibody H3-1 and the Saccharomyces cerevisiae alpha-factor pre-pro-leader sequence (pre-pro-alpha-F) were used as models. The use of pre-region of the pre-pro-alpha-F alone or together with the Glu-Ala-linker leads to the slightly increased yield of the secreted L-chain but was accompanied by the incomplete N-terminal processing of the secreted product.
Kozlov
D G
DG
State Research Institute for Genetics and Selection of Industrial Microorganisms, 117545 Moscow, Russia. dg_kozlov@genetika.ru
Yagudin
T A
TA
D000595:Amino Acid Sequence; D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D007128:Immunoglobulin Fragments / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D008969:Molecular Sequence Data; D010843:Pichia / Q000378:metabolism*; D011498:Protein Precursors / Q000737:chemistry; D011499:Protein Processing, Post-Translational
D007128:Immunoglobulin Fragments; D011498:Protein Precursors