title: Detection of carbonyl groups in triterpenoids by hydroxylamine hydrochloride derivatization using electrospray ionization mass spectrometry.
abstract: A rapid, simple electrospray ionization mass spectrometry (ESI-MS) sensitivity enhancement method for detecting carbonyl groups in triterpenoids has been developed by using hydroxylamine hydrochloride (NH(2)OH . HCl) as a derivatization reagent. We use the oxime formed during the derivatization reactions and its Beckmann rearrangement intermediates as a means of detecting the carbonyl groups originally present in these triterpenoids. In comparison with other derivatization methods reported in the literature, this method is simple, specific and can be used to detect carbonyl groups in triterpenoids which have low polarity and are poorly or non-ionizable. Moreover, it can also be used to detect hydroxyl groups by using the Dess-Martin periodinane (DMP) to convert primary and secondary hydroxyls into carbonyl groups.
authors.affiliation: Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan Province, 610041, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.forename: Haibin
authors.initials: H
authors.lastname: Chen
authors.forename: Shulin
authors.initials: S
authors.lastname: Peng
authors.forename: Yucheng
authors.initials: Y
authors.lastname: Gu
authors.forename: Lisheng
authors.initials: L
authors.lastname: Ding
mesh_terms: D019811:Hydroxylamine / Q000737:chemistry*; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000737:chemistry; D028321:Plant Preparations / Q000737:chemistry; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013729:Terpenes / Q000737:chemistry*; D013997:Time Factors
chemical_list: D028321:Plant Preparations; D013729:Terpenes; D019811:Hydroxylamine; D009828:Oleanolic Acid