A new pancreatic lipase inhibitor isolated from the roots of Actinidia arguta.
A new coumaroyl triterpene, 3-O-trans-p-coumaroyl actinidic acid (1), as well as five known triterpenes, ursolic acid (2), 23-hydroxyursolic acid (3), corosolic acid (4), asiatic acid (5) and betulinic acid (6) were isolated from an EtOAc-soluble extract of the roots of Actinidia arguta. The structure of compound 1 was elucidated from interpretation of the spectroscopic data, particularly by extensive 1D and 2D NMR studies. All the isolates (1-6) were evaluated in vitro for their inhibitory activities on pancreatic lipase (PL). Of the isolates, the new compound 1 possessed the highest inhibitory activity on PL, with an IC(50) of 14.95 microM, followed by ursolic acid (2, IC(50) = 15.83 microM). The other four triterpenes (3-6) also showed significant PL inhibitory activity, with IC(50) values ranging from 20.42 to 76.45 microM.
Jang
Dae Sik
DS
Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, Daejeon, 305-811, Korea.
Lee
Ga Young
GY
Kim
Junghyun
J
Lee
Yun Mi
YM
Kim
Jong Min
JM
Kim
Young Sook
YS
Kim
Jin Sook
JS
D029042:Actinidia; D000818:Animals; D008049:Lipase / Q000037:antagonists & inhibitors* / Q000737:chemistry; D010179:Pancreas / Q000201:enzymology*; D010936:Plant Extracts / Q000737:chemistry; D018517:Plant Roots; D013552:Swine; D014315:Triterpenes / Q000302:isolation & purification* / Q000494:pharmacology
C530811:3-O-p-coumaroyl actinidic acid; D010936:Plant Extracts; D014315:Triterpenes; D008049:Lipase