Development of a conjugated gadolinium and cisplatin-gelatin possessing properties as an intravascular contrast agent for MR imaging.
OBJECTIVE
The purpose of this study is to create a Gd-DTPA-Gel-Cis compound, which made from gadolinum (Gd), diethylenetriaminepentaacetic acid (DTPA)-dianhydride, cis-diamminedichloroplatinum (Cis) and bovine gelatin (Gel), that makes it possible to visualize Cis as intravascular agent under magnetic resonance imaging (MRI).


METHODS
The amount of DTPA, Gd, and Cis were titrated to determine the new compound's conjugation ratio with gelatin. Considering these functions, Gd-DTPA-Gel-Cis was synthesized, and its stability in bovine serum was evaluated. In addition, the signal intensity of the diluted sample was measured under 1.5 Tesla MRI.


RESULTS
The synthesized 10mg/ml of Gd-DTPA-Gel-Cis contained 42.84 microg/ml of Gd and 1.53 microg/ml of platinum. Gd-DTPA-Gel-Cis (100 mg/10 ml) enclosed into the cellulose dialysis tubing was placed in 90 ml of bovine serum and shaken reciprocally at 72 stroke/min at 37 degrees C. Partial release of free Pt was shown at 6 and 24 h, but no release of Gd occurred for a 24-h period. And high stability of Gd conjugated to DTPA-Gel-Cis. This result suggests possible anti-tumor effectiveness and high stability of Gd conjugated to DTPA-Gel-Cis. The diluted sample presented high signal intensity under 1.5 Tesla MRI.


CONCLUSIONS
Gd-DTPA-Gel-Cis has been developed successfully and we have proven its stability and contrast ability in MRI.
Sonoda
Akinaga
A
Department of Radiology, Shiga University of Medical Science, Setatsukinowa-cho, Otsu, Shiga 520-2192, Japan. akinagasonoda@yahoo.co.jp
Nitta
Norihisa
N
Ohta
Shinichi
S
Seko
Ayumi
A
Jo
Jun-ichiro
J
Morikawa
Shigehiro
S
Tabata
Yasuhiko
Y
Takahashi
Masashi
M
Murata
Kiyoshi
K
D002945:Cisplatin / Q000737:chemistry* / Q000627:therapeutic use*; D003287:Contrast Media / Q000138:chemical synthesis*; D005682:Gadolinium / Q000737:chemistry*; D005780:Gelatin / Q000737:chemistry; D007089:Image Enhancement / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*
D003287:Contrast Media; D005780:Gelatin; D005682:Gadolinium; D002945:Cisplatin