title: Fungicidal activity of human monocyte-derived multinucleated giant cells induced in vitro by Paracoccidioides brasiliensis antigen.
abstract: Multinucleated giant cells (MGC) are characteristic cells in granulomatous disorders such as paracoccidioidomycosis (PCM) and also are formed in vitro from peripheral blood mononuclear cells by several stimuli. In this study, the authors investigated in vitro formation of MGC derived from monocytes of healthy individuals, stimulated with Paracoccidioides brasiliensis antigen (PbAg), compared with other stimuli such as IFN-gamma and supernatant of Con-A-stimulated peripheral blood mononuclear cells (CM-ConA). Besides, the fungicidal activity of monocytes and monocyte-derived MGC challenged with P. brasiliensis were compared, at a ratio of one fungus per 50 monocytes. Results demonstrated that PbAg, IFN-gamma, and CM-ConA stimuli were able to induce MGC generation, with fusion indices significantly higher than control cultures. Striking results were observed when MGC induced by PbAg and IFN-gamma presented higher fungicidal activity than monocytes, submitted to the same stimuli, showing a better capacity of these cells to kill P. brasiliensis. In summary, the results suggest that PbAg is able to induce MGC generation, and these cells presented higher fungicidal activity against P. brasiliensis than monocytes.
authors.affiliation: Department of Microbiology and Immunology, Biosciences Institute, São Paulo State University (UNESP), CEP 18618-000, Botucatu, São Paulo, Brazil.
authors.forename: Magda Paula Pereira
authors.initials: MP
authors.lastname: do Nascimento
authors.forename: Angela Maria Victoriano
authors.initials: AM
authors.lastname: de Campos Soares
authors.forename: Luciane Alarcão
authors.initials: LA
authors.lastname: Dias-Melicio
authors.forename: Maria Rita
authors.initials: MR
authors.lastname: Parise-Fortes
authors.forename: Rosana Aparecida Rodrigues
authors.initials: RA
authors.lastname: Martins
authors.forename: Erika Takahashi
authors.initials: ET
authors.lastname: Nakaira
authors.forename: Maria Terezinha Serrão
authors.initials: MT
authors.lastname: Peraçoli
mesh_terms: D000328:Adult; D000946:Antigens, Fungal / Q000008:administration & dosage; D002459:Cell Fusion; D017077:Culture Media, Conditioned; D015726:Giant Cells / Q000166:cytology / Q000276:immunology* / Q000382:microbiology*; D006801:Humans; D066298:In Vitro Techniques; D007371:Interferon-gamma / Q000494:pharmacology; D018942:Macrolides / Q000494:pharmacology; D008875:Middle Aged; D009000:Monocytes / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D010228:Paracoccidioides / Q000276:immunology* / Q000472:pathogenicity; D010229:Paracoccidioidomycosis / Q000276:immunology / Q000382:microbiology; D011994:Recombinant Proteins
chemical_list: D000946:Antigens, Fungal; D017077:Culture Media, Conditioned; D018942:Macrolides; D011994:Recombinant Proteins; C076477:concanamycin F; D007371:Interferon-gamma