title: Atmospheric pressure photoionization mass spectrometry of oligodeoxyribonucleotides.
abstract: Small oligonucleotides (di- and trimers) were investigated by atmospheric pressure photoionization (APPI) with focus on the fragmentation mechanisms. The fragmentation patterns of these biomolecular ions have been monitored under dopant-assisted photoionization (DA-APPI) conditions. Our results reveal new aspects of the gas- phase chemistry of ions formed from such biomolecules. They illustrate that the reaction between low-energy electrons released from photoionization processes and di- and trinucleotides lies in dissociative electron attachment processes leading to phosphodiester bond cleavages and to the formation of numerous fragments in the ion source. The conditions of DA-APPI, which involve protic solvents and atmospheric pressure conditions, seem to be relevant for the study of radiation damages to biological molecules.
authors.affiliation: Laboratoire de Spectrométrie de Masse, ICSN-CNRS, Avenue de la Terrasse, 91190 Gif-sur-Yvette, France.
authors.forename: A
authors.initials: A
authors.lastname: Bagag
authors.forename: A
authors.initials: A
authors.lastname: Giuliani
authors.forename: O
authors.initials: O
authors.lastname: Laprévote
mesh_terms: D000838:Anions / Q000737:chemistry; D001274:Atmospheric Pressure; D053960:DNA Breaks; D004583:Electrons; D013058:Mass Spectrometry; D009838:Oligodeoxyribonucleotides / Q000737:chemistry*; D010777:Photochemistry
chemical_list: D000838:Anions; D009838:Oligodeoxyribonucleotides