The polymorphism of XRCC3 codon 241 and AFB1-related hepatocellular carcinoma in Guangxi population, China.
OBJECTIVE
The relationship between aflatoxin B1 (AFB1) exposure and hepatocellular carcinoma (HCC) has been previously demonstrated and supported with strong epidemiological evidence. However, the role of genetic polymorphism of X-ray cross-complementing group 3 (XRCC3) codon 241 (namely: Thr241Met), which may be involved in the repair of DNA double-strand breaks caused by carcinogens such as AFB1, been less well elaborated.


METHODS
We conducted a case-control study including 491 cases and 862 controls to evaluate the associations between this polymorphism and HCC risk for Guangxi population by means of polymerase chain reaction-restriction fragment length polymorphism analysis.


RESULTS
We found that individuals with the XRCC3 genotypes with codon 241 Met (namely XRCC3-TM or XRCC3-MM) had an increased risk of HCC than those with the homozygote of XRCC3 codon 241 Thr alleles (namely XRCC3-TT, adjusted odds ratios 2.22 and 7.19; 95% confidence intervals 1.72-2.88 and 4.52-11.42, respectively). The risk of HCC, moreover, did appear to differ more significantly among individuals featuring high-level AFB1-DNA adducts, whose adjusted odds ratios (95% confidence intervals) were 11.59 (5.73-23.47) and 37.54 (16.32-86.32), respectively.


CONCLUSIONS
These findings support the hypothesis that the XRCC3 Thr241Met polymorphism may be associated with the risk of AFB1-related HCC among the Guangxi population.
Long
Xi Dai
XD
Department of Pathology, Youjiang Medical College for Nationalities, Baise, Guangxi Zhuang Autonomous Region, China. xiaolonglong200166@yahoo.com.cn
Ma
Yun
Y
Qu
De Ying
DY
Liu
Yun Guang
YG
Huang
Zhao Quan
ZQ
Huang
Yong Zhi
YZ
Lin
Zhong Hui
ZH
Wei
Ni Bo
NB
Zhou
Shu Chun
SC
D000328:Adult; D016604:Aflatoxin B1 / Q000506:poisoning*; D000368:Aged; D006528:Carcinoma, Hepatocellular / Q000139:chemically induced* / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D003062:Codon; D004249:DNA Damage; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008113:Liver Neoplasms / Q000139:chemically induced* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic
D003062:Codon; D004268:DNA-Binding Proteins; C110901:X-ray repair cross complementing protein 3; D016604:Aflatoxin B1