Review of an in vitro microfluidic model of sickle cell vaso-occlusion.
Vaso-occlusive events are responsible for the majority of morbidity and mortality in sickle cell disease. Predisposing conditions are unclear, and proximal causes have not been established. Despite decades of intense study, until recently there has not been a well-controlled in vitro model of sickle cell vaso-occlusion. We have reported the development and initial use of such a model. Our experimental device relies on microfluidic technology and has allowed the initial analysis of the in vitro process of vaso-occlusion in terms of control parameters such as driving pressure, local oxygen concentration and flow vessel size. Our work demonstrates the potential of this type of device to lead to greater understanding of vaso-occlusive pathology including the role of adhesion molecules and inflammatory factors and possibly to improvements in monitoring and searches for new treatments.
Higgins
J M
JM
School of Engineering and Applied Sciences, Harvard University, 29, Oxford Street, Cambridge, MA 02138, USA. jmhiggins@partners.org
Eddington
D T
DT
Bhatia
S N
SN
Mahadevan
L
L
D000755:Anemia, Sickle Cell / Q000473:pathology / Q000503:physiopathology*; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation*; D008961:Models, Structural