IL-1beta and IL-6 modulate apolipoprotein E gene expression in rat hepatocyte primary culture.
Incubation of rat hepatocytes in primary culture with IL-1beta at a concentration of 2.5 units/ml resulted in an increase (+80%) in the amount of apoE mRNA without any effect upon apoE synthesis. IL-6 at a low concentration (10 units/ml) induced a decrease (-35%) in the amount of apoE mRNA, but increased apoE synthesis (+28%). No effect was observed with higher concentrations of IL-1beta (10 units/ml) or IL-6 (100 units/ml). These results suggest that inflammatory cytokines IL-1beta and IL-6 modulate the expression of apoE gene in cultured rat hepatocytes, at a concentration that does not induce the acute phase response.
Ribeiro
A
A
CNRS URA 1283 CHU Saint Antoine 27 rue de Chaligny Paris 75012 France.
Mangeney
M
M
Bernard
I
I
Chambaz
J
J
Delers
F
F