title: PLP catabolism: identification of the 2-(Acetamidomethylene)succinate hydrolase gene in Mesorhizobium loti MAFF303099.
abstract: The function of the mlr6787 gene from Mesorhizobium loti MAFF303099 has been identified. This gene encodes 2-(acetamidomethylene)succinate hydrolase, an enzyme involved in the catabolism of pyridoxal 5'-phosphate (vitamin B 6). This enzyme was overexpressed in Escherichia coli, purified to homogeneity, and characterized. 2-(Acetamidomethylene)succinate hydrolase catalyzes the hydrolysis of 2-(acetamidomethylene)succinate to yield succinic semialdehyde, acetic acid, carbon dioxide, and ammonia. The k cat and K M for this reaction were 0.6 s (-1) and 143 microM, respectively. The enzyme was shown to utilize the E isomer of 2-(acetamidomethylene)succinate.
authors.affiliation: Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.
authors.forename: Tathagata
authors.initials: T
authors.lastname: Mukherjee
authors.forename: David G
authors.initials: DG
authors.lastname: Hilmey
authors.forename: Tadhg P
authors.initials: TP
authors.lastname: Begley
mesh_terms: D020561:Alphaproteobacteria / Q000201:enzymology* / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D017931:DNA Primers; D004926:Escherichia coli / Q000201:enzymology; D006867:Hydrolases / Q000378:metabolism*; D007700:Kinetics; D011549:Pseudomonas / Q000201:enzymology; D011732:Pyridoxal Phosphate / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D050644:Succinate-Semialdehyde Dehydrogenase / Q000302:isolation & purification / Q000378:metabolism; D005680:gamma-Aminobutyric Acid / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D017931:DNA Primers; D011994:Recombinant Proteins; D005680:gamma-Aminobutyric Acid; D011732:Pyridoxal Phosphate; D050644:Succinate-Semialdehyde Dehydrogenase; C530614:2-(acetamidomethylene)succinate hydrolase, Mesorhizobium loti; D006867:Hydrolases; C009338:succinic semialdehyde