The use of experimental murine models to assess novel agents of hematopoietic stem and progenitor cell mobilization.
The recent explosion in the understanding of the cellular and molecular mechanisms underlying hematopoietic stem and progenitor cell (HSPC) mobilization has facilitated development of novel therapeutic agents, targeted at improving mobilization kinetics as well as HSPC yield. With the development of new agents comes the challenge of choosing efficient and relevant preclinical studies for the testing of the HSPC mobilization efficacy of these agents. This article reviews the use of the mouse as a convenient small animal model of HSPC mobilization and transplantation, and outlines the range of murine assays that can be applied to assess novel HSPC mobilizing agents. Techniques to demonstrate murine HSPC mobilization are discussed, as well as the role of murine assays to confirm human HSPC mobilization, and techniques to investigate the biologic phenotype of HSPC mobilized by these novel agents. Technical aspects regarding mobilization regimens and control arms, and choice of experimental animals are also discussed.
Herbert
Kirsten E
KE
Peter MacCallum Cancer Centre, East Melbourne, Victoria, Australia. kirsten.herbert@petermac.org
Lévesque
Jean-Pierre
JP
Haylock
David N
DN
Prince
H Miles
HM
Prince
Miles
M
D000818:Animals; D020296:Animals, Congenic; D018929:Cell Culture Techniques / Q000379:methods; D019070:Cell Lineage; D002478:Cells, Cultured / Q000187:drug effects; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005260:Female; D006085:Graft Survival; D016298:Hematopoietic Cell Growth Factors / Q000494:pharmacology; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods*; D018380:Hematopoietic Stem Cell Transplantation; D006412:Hematopoietic Stem Cells / Q000145:classification / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008297:Male; D051379:Mice; D016688:Mice, Inbred NOD; D008815:Mice, Inbred Strains; D016513:Mice, SCID; D023421:Models, Animal; D019653:Myeloablative Agonists / Q000494:pharmacology; D011828:Radiation Chimera
D016298:Hematopoietic Cell Growth Factors; D019653:Myeloablative Agonists