MHC class I characterization of Indonesian cynomolgus macaques.
Cynomolgus macaques (Macaca fascicularis) are quickly becoming a useful model for infectious disease and transplantation research. Even though cynomolgus macaques from different geographic regions are used for these studies, there has been limited characterization of full-length major histocompatibility complex (MHC) class I immunogenetics of distinct geographic populations. Here, we identified 48 MHC class I cDNA nucleotide sequences in eleven Indonesian cynomolgus macaques, including 41 novel Mafa-A and Mafa-B sequences. We found seven MHC class I sequences in Indonesian macaques that were identical to MHC class I sequences identified in Malaysian or Mauritian macaques. Sharing of nucleotide sequences between these geographically distinct populations is also consistent with the hypothesis that Indonesia was a source of the Mauritian macaque population. In addition, we found that the Indonesian cDNA sequence Mafa-B7601 is identical throughout its peptide binding domain to Mamu-B03, an allele that has been associated with control of Simian immunodeficiency virus (SIV) viremia in Indian rhesus macaques. Overall, a better understanding of the MHC class I alleles present in Indonesian cynomolgus macaques improves their value as a model for disease research, and it better defines the biogeography of cynomolgus macaques throughout Southeast Asia.
Pendley
Chad J
CJ
Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, WI 53706, USA.
Becker
Ericka A
EA
Karl
Julie A
JA
Blasky
Alex J
AJ
Wiseman
Roger W
RW
Hughes
Austin L
AL
O'Connor
Shelby L
SL
O'Connor
David H
DH
D000483:Alleles; D000818:Animals; D005805:Genes, MHC Class I; D005828:Genetics, Population; D007214:Indonesia; D008252:Macaca fascicularis / Q000235:genetics* / Q000276:immunology