In vitro characterization of borneol metabolites by GC-MS upon incubation with rat liver microsomes.
The metabolism of borneol is studied by the analysis of incubations of in vitro-prepared rat liver microsomes. A sensitive gas chromatography (GC)-mass spectrometry (MS) method is developed for the identification of borneol and its metabolites. Four novel metabolites, which have not previously been reported, are isolated and confirmed by comparison of the GC-MS method. The biotransformation pathway of borneol in rat liver microsomes is proposed based on the in vitro results.
Zhang
Rong
R
Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Jichang Road 12, Guangzhou 510405, Guangdong Province, PR China.
Liu
Chang-hui
CH
Huang
Tian-lai
TL
Wang
Ning-sheng
NS
Mi
Sui-qing
SQ
D000818:Animals; D001892:Camphanes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D066298:In Vitro Techniques; D008297:Male; D008862:Microsomes, Liver / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley
D001892:Camphanes; C022871:isoborneol