Expression of Cdx2 and claudin-2 in the multistage tissue of gastric carcinogenesis.
In this paper, we investigated Cdx2 and claudin-2 expression in pathological paraffin tissues of sinus ventriculi from gastroscopic biopsy to determine the correlation between the expressions of these 2 genes during gastric carcinogenesis by immunochemical ABC technique. Altogether, we analyzed 108 chronic superficial gastritis, 55 chronic atrophic gastritis, 109 intestinal-type metaplasia, 93 dysplasia and 52 gastric intestinal-type adenocarcinoma samples. Our results indicated that the percentage of Cdx2-positive cases was 0% (0/108) for chronic superficial gastritis, 0% (0/55) for chronic atrophic gastritis, 90.83% (99/109) for intestinal-type metaplasia, 51.61% (48/93) for dysplasia and 61.54% (32/52) for gastric intestinal-type adenocarcinoma, primarily expressed in the cell nucleus and partly in the cytoplasm (p < 0.05); interestingly, the percentage for the intestine-type metaplasia was markedly high. The percentage of claudin-2-positive cases was 0% (0/108) for chronic superficial gastritis, 0% (0/55) for chronic atrophic gastritis, 0% (0/109) for intestinal-type metaplasia, 35.48% (33/93) for dysplasia and 71.15% (37/52) for gastric intestinal-type adenocarcinoma, primarily in the cell membrane and gradually increased in the multistage process of gastric carcinogenesis (p < 0.05). Significant correlations were found between claudin-2 and Cdx2 protein expression in dysplasia and intestine-type adenocarcinoma (r = 0.112, p < 0.05). Thus, there may be a correlation between the expression of claudin-2 and Cdx2 in stages of dysplasia and cancer.
Xin
Song
S
Department of Gastroenterology, Key Laboratory of Nephrology of the Ministry of Education and the Ministry of Health, First Affiliated Hospital, Sun-Yat Sen University, Guangzhou, SAR, China.
Huixin
Chen
C
Benchang
Shen
S
Aiping
Bai
B
Jinhui
Wang
W
Xiaoyan
Li
L
Yu
Wong Benjamin Chun
WB
Minhu
Chen
C
D000230:Adenocarcinoma / Q000473:pathology*; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000071616:CDX2 Transcription Factor; D057167:Claudins; D005260:Female; D005756:Gastritis / Q000473:pathology; D018398:Homeodomain Proteins / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D007414:Intestinal Neoplasms / Q000473:pathology*; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008679:Metaplasia / Q000469:parasitology; D008875:Middle Aged; D009367:Neoplasm Staging; D013274:Stomach Neoplasms / Q000473:pathology*
D000071616:CDX2 Transcription Factor; C491270:CDX2 protein, human; C491875:CLDN2 protein, human; D057167:Claudins; D018398:Homeodomain Proteins; D008565:Membrane Proteins