Temperature-dependent parasitic relationship between Legionella pneumophila and a free-living amoeba (Acanthamoeba castellanii).
We analyzed the effects of temperature on the interaction of Legionella pneumophila with Acanthamoeba castellanii. At <20 degrees C, overexpression of type 1 metacaspase, a stimulator of A. castellanii encystation, was associated with a reduced number of bacteria within amoeba. At low temperatures, A. castellanii seems to eliminate L. pneumophila by encystation and digestion.
Ohno
Akira
A
Department of Microbiology and Infectious Disease, Faculty of Medicine, Toho University, 5-21-16 Omori-Nishi, Ota-Ku, Tokyo 143-8540, Japan. akira@med.toho-u.ac.jp
Kato
Naoyuki
N
Sakamoto
Ryota
R
Kimura
Soichiro
S
Yamaguchi
Keizo
K
D048248:Acanthamoeba castellanii / Q000254:growth & development / Q000382:microbiology*; D000818:Animals; D001426:Bacterial Proteins / Q000378:metabolism; D020169:Caspases / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D054884:Host-Pathogen Interactions; D016952:Legionella pneumophila / Q000254:growth & development / Q000502:physiology*; D015800:Protozoan Proteins / Q000378:metabolism; D012329:RNA, Bacterial / Q000302:isolation & purification; D016053:RNA, Protozoan / Q000302:isolation & purification; D012097:Repressor Proteins / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013696:Temperature; D053058:Trophozoites / Q000254:growth & development; D014871:Water Microbiology
D001426:Bacterial Proteins; C488628:CsrA protein, Legionella pneumophila; D015800:Protozoan Proteins; D012329:RNA, Bacterial; D016053:RNA, Protozoan; D012097:Repressor Proteins; C081902:pmrA protein, Bacteria; C085504:CpxR protein, Bacteria; D020169:Caspases