Determination of vitamin E and carotenoid pigments by high performance liquid chromatography in shell of Chionoecetes opilio.
This study reports the optimization of a method for the determination of vitamin E and carotenoids in shells of Chionoecetes opilio samples by online HPLC coupled with UV-vis and fluorescence detectors. The carotenoids were determined with diode-array detector (lambda 450 nm) and vitamin E with fluorescence detection (lambda(ex) 288, lambda(em) 331 nm). Two extractions methods were compared, saponification followed by an extraction step and a simple extraction with acetone. The last one was selected because allows to determine all compounds. Linearity, precisions and recoveries achieved for all compounds were satisfactory. Mean concentrations (mg per 100 g dry weight) were; 23.3 for vitamin E, 9.49 for astaxanthin and 0.2 mg for beta-carotene.
Vilasoa-Martínez
María
M
Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of Pharmacy, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Calaza-Ramos
Carina
C
López-Hernández
Julia
J
Lage-Yusty
María Asunción
MA
Losada
Perfecto Paseiro
PP
Rodríguez-Bernaldo de Quirós
Ana
A
D000818:Animals; D003386:Brachyura / Q000737:chemistry* / Q000145:classification*; D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D010860:Pigments, Biological / Q000032:analysis*; D014810:Vitamin E / Q000032:analysis*
D010860:Pigments, Biological; D014810:Vitamin E; D002338:Carotenoids