Effect of aeration and agitation on the protease production by Staphylococcus aureus mutant RC128 in a stirred tank bioreactor.
The modified rotating simplex method has been successfully used to determine the best combination of agitation rate and aeration rate for maximum production of extracellular proteases by Staphylococcus aureus mutant RC128, in a stirred tank bioreactor operated in a discontinuous way. This mutant has shown altered exoprotein production, specially enhanced protease production. Maximum production of proteases (15.28 UP/ml), measured using azocasein as a substrate, was obtained at exponential growth phase when the bioreactor was operated at 300 rpm and at 2 vvm with a volumetric oxygen transfer coefficient (K(L)a) of 175.75 h(-1). These conditions were found to be more suitable for protease production.
Ducros
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Departamento de Tecnología Química, Facultad de Ingeniería, Universidad Nacional de Río Cuarto, Río Cuarto, Argentina. educros@ing.unrc.edu.ar
Ferrari
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M
Pellegrino
M
M
Raspanti
C
C
Bogni
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C
D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002138:Calibration; D002364:Caseins / Q000737:chemistry; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology / Q000295:instrumentation / Q000379:methods; D015233:Models, Statistical; D009154:Mutation; D010100:Oxygen / Q000737:chemistry; D010447:Peptide Hydrolases / Q000737:chemistry; D013211:Staphylococcus aureus / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D002364:Caseins; D003470:Culture Media; C026018:azocasein; D010447:Peptide Hydrolases; D010100:Oxygen