title: Implication of connexins 40 and 43 in functional coupling between mouse cardiac fibroblasts in primary culture.
abstract: Cardiac fibroblasts contribute to the structure and function of the myocardium. However their involvement in electrophysiological processes remains unclear; particularly in pathological situations when they proliferate and develop fibrosis. We have identified the connexins involved in gap junction channels between fibroblasts from adult mouse heart and characterized their functional coupling. RT-PCR and Western blotting results show that mRNA and proteins of connexin40 and connexin43 are expressed in cultured cardiac fibroblasts, while Cx45 is not detected. Analysis of gap junctional communications established by these connexins with the gap-FRAP technique demonstrates that fibroblasts are functionally coupled. The time constant of permeability, k, calculated from the fluorescence recovery curves between cell pairs is 0.066+/-0.005 min(-1) (n = 65). Diffusion analysis of Lucifer Yellow through gap junction channels with the scrape-loading method demonstrates that when they are completely confluent, a majority of fibroblasts are coupled forming an interconnecting network over a distance of several hundred micrometers. These data show that cardiac fibroblasts express connexin40 and connexin43 which are able to establish functional communications through homo and/or heterotypic junctions to form an extensive coupled cell network. It should then be interesting to study the conditions to improve efficiency of this coupling in pathological conditions.
authors.affiliation: Institut de Physiologie et Biologie Cellulaires, Université de Poitiers, CNRS UMR 6187, 40 Avenue du Recteur Pineau, 86022 Poitiers Cedex, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Louault
authors.forename: Najate
authors.initials: N
authors.lastname: Benamer
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Faivre
authors.forename: Daniel
authors.initials: D
authors.lastname: Potreau
authors.forename: Jocelyn
authors.initials: J
authors.lastname: Bescond
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018031:Connexin 43 / Q000235:genetics / Q000378:metabolism*; D017630:Connexins / Q000235:genetics / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D017629:Gap Junctions / Q000737:chemistry / Q000378:metabolism*; D006329:Heart Conduction System / Q000378:metabolism*; D051379:Mice; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D000097003:Gap Junction alpha-5 Protein
chemical_list: D018031:Connexin 43; D017630:Connexins; D000097003:Gap Junction alpha-5 Protein