title: Community structure of Phialocephala fortinii s. lat. in European tree nurseries, and assessment of the potential of the seedlings as dissemination vehicles.
abstract: Patterns of colonization of conifer roots by dark septate endopyhtes of the Phialocephala fortinii s. lat. species complex in nurseries in Switzerland and Lithuania were studied. The potential for man-mediated genotype flow was estimated for two Swiss nurseries based on customers' addresses and the number of delivered plants. Two hundred and forty-nine strains from three Swiss and five Lithuanian nurseries and an afforestation site were characterized using a combination of inter-simple sequence repeat-anchored PCR (ISSR-PCR), single-copy RFLP analysis, and sequence analysis. P. fortinii s. lat. was abundant in nursery seedlings, but the frequency of seedlings colonized varied considerably among and within nurseries. Ten cryptic species (CSP) of P. fortinii s. lat. were identified, including four hitherto undiscovered CSP. P. helvetica was the dominant species in Swiss nurseries, whereas P. fortinii s. str. was the most abundant species in Lithuanian nurseries and the afforestation site. Swiss nurseries deliver plants over distances of more than 200km indicating the high potential for man-mediated genotype flow in P. fortinii s. lat.
authors.affiliation: ETH Zürich, Institute of Integrative Biology (IBZ), Forest Pathology and Dendrology, Zürich, Switzerland.
authors.forename: Nina
authors.initials: N
authors.lastname: Brenn
authors.forename: Audrius
authors.initials: A
authors.lastname: Menkis
authors.forename: Christoph R
authors.initials: CR
authors.lastname: Grünig
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Sieber
authors.forename: Ottmar
authors.initials: O
authors.lastname: Holdenrieder
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004271:DNA, Fungal / Q000235:genetics; D008097:Lithuania; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000382:microbiology; D012150:Polymorphism, Restriction Fragment Length; D036226:Seedlings / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D013557:Switzerland; D014197:Trees / Q000382:microbiology*
chemical_list: D004271:DNA, Fungal