Covalent inhibitors of glycosidases and their applications in biochemistry and biology.
Glycoside hydrolases are important enzymes in a number of essential biological processes. Irreversible inhibitors of this class of enzyme have attracted interest as probes of both structure and function. In this review we discuss some of the compounds used to covalently modify glycosidases, their use in residue identification, structural and mechanistic investigations, and finally their applications, both in vitro and in vivo, to complex biological systems.
Rempel
Brian P
BP
Department of Chemistry, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Withers
Stephen G
SG
D000818:Animals; D001671:Biochemistry; D001695:Biology; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D017611:Fluorine Compounds / Q000737:chemistry / Q000494:pharmacology; D006026:Glycoside Hydrolases / Q000037:antagonists & inhibitors* / Q000145:classification / Q000378:metabolism*; D006801:Humans; D011485:Protein Binding
D004791:Enzyme Inhibitors; D017611:Fluorine Compounds; D006026:Glycoside Hydrolases