Upregulation of PSCDBP, TLR2, TWIST1, FLJ35382, EDNRB, and RGS12 gene expression in human myometrium at labor.
The regulatory mechanisms underlying myometrial smooth muscle contractility during labor are poorly understood. The authors therefore investigated the transcriptional profile of the changes that occur in the human myometrium at term pregnancy when compared with that at labor. Microarray technology was used to identify differentially expressed genes in human myometrium at labor. Real-time fluorescence reversetranscriptase polymerase chain reaction (RT-PCR) was subsequently performed to verify the microarray data. Semiquantitative RT-PCR, Western blotting, and microscopy methodologies were also used. Certain novel genes were found to be upregulated in human myometrium at labor. Of these, PSCDBP, TLR2, TWIST1 , FLJ35382, andRGS12 have not been previously characterized or identified in human myometrium. EDNRB is the other novel labor-associated gene whose reported expression is also upregulated at labor. All 6 genes were expressed on human myometrial smooth muscle cells. These novel upregulated genes are involved in multiple pathways that may be associated with a variety of cellular processes including inflammation, transcriptional regulation, and intracellular signaling.
O'Brien
Margaret
M
National Centre for Biomedical and Engineering Science, National University of Ireland Galway, Galway, Ireland. margaret.obrien@nuigalway.ie
Morrison
John J
JJ
Smith
Terry J
TJ
D000328:Adult; D015153:Blotting, Western; D002478:Cells, Cultured; D002585:Cesarean Section; D017931:DNA Primers; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007743:Labor, Obstetric / Q000235:genetics* / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D009215:Myometrium / Q000166:cytology / Q000378:metabolism* / Q000601:surgery; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011247:Pregnancy; D020710:RGS Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D044023:Receptor, Endothelin B / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D047929:Term Birth / Q000235:genetics / Q000378:metabolism; D051195:Toll-Like Receptor 2 / Q000235:genetics* / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D051879:Twist-Related Protein 1 / Q000235:genetics* / Q000378:metabolism; D015854:Up-Regulation
C084539:CYTIP protein, human; D017931:DNA Primers; D009687:Nuclear Proteins; D020710:RGS Proteins; C106375:RGS12 protein, human; D012333:RNA, Messenger; D044023:Receptor, Endothelin B; C495342:TLR2 protein, human; C103173:TWIST1 protein, human; D051195:Toll-Like Receptor 2; D014157:Transcription Factors; D051879:Twist-Related Protein 1