Determination of proteins and carbohydrates in the effluents from wastewater treatment bioreactors using resonance light-scattering method.
A simple and sensitive method was developed for the determination of low-concentration proteins and carbohydrates in the effluents from biological wastewater treatment reactors using resonance light-scattering (RLS) technique. Two ionic dyes, Congo red and Neutral red were, respectively used as an RLS probes for the determination of proteins and carbohydrates. This method is based on the interactions between biomacromolecules and dyes, which cause a substantial increase in the resonance scattering signal of dyes in the wavelength range of 200-650 nm. The characteristics of RLS spectra of the macromolecule-dye complexes, influencing factors, and optimum analytical conditions for the measurement were explored. The method was satisfactorily applied to the measurement of proteins and carbohydrates in the effluents from 10 aerobic or anaerobic bioreactors, and a high sensitivity were achieved.
Zhang
Meng-Lin
ML
Department of Chemistry, University of Science & Technology of China, Hefei, China.
Sheng
Guo-Ping
GP
Yu
Han-Qing
HQ
D019149:Bioreactors; D002138:Calibration; D002241:Carbohydrates / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D008027:Light; D015394:Molecular Structure; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D014865:Waste Disposal, Fluid / Q000379:methods*; D014867:Water / Q000032:analysis*
D002241:Carbohydrates; D011506:Proteins; D014867:Water