title: Low-dose oral methotrexate induces apoptosis of tissue eosinophils in bullous pemphigoid.
abstract: We have shown previously that eosinophils are abundant in blood and tissue in bullous pemphigoid and that they rapidly disappear, without sign of necrosis, during methotrexate therapy. The aim of the present study was to investigate the role of apoptosis in this phenomenon. The terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick-end labelling method for detecting apoptosis was used, combined with immunohistochemical detection of eosinophils before and during oral methotrexate. The numbers of apoptotic eosinophils were compared using light microscopy. Eosinophil apoptosis was significantly higher in skin after one week of methotrexate therapy and the number of eosinophils was reduced. The results indicate that methotrexate therapy induces apoptosis of the tissue eosinophils in bullous pemphigoid.
authors.affiliation: Department of Dermatology, Karolinska University Hospital, Solna, Stockholm, Sweden and 2Hospital Archet II, INSERM U385, Nice, France. kghozlan@bredband.net
authors.forename: Kristina
authors.initials: K
authors.lastname: Dahlman-Ghozlan
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Ortonne
authors.forename: Philippe
authors.initials: P
authors.lastname: Bahadoran
authors.forename: Anne
authors.initials: A
authors.lastname: Spadafora
authors.forename: Eija
authors.initials: E
authors.lastname: Stephansson
mesh_terms: D000284:Administration, Oral; D017209:Apoptosis / Q000187:drug effects*; D001706:Biopsy; D004804:Eosinophils / Q000187:drug effects / Q000473:pathology*; D006801:Humans; D007150:Immunohistochemistry; D007166:Immunosuppressive Agents / Q000008:administration & dosage*; D020287:In Situ Nick-End Labeling; D008727:Methotrexate / Q000008:administration & dosage*; D008853:Microscopy; D010391:Pemphigoid, Bullous / Q000188:drug therapy / Q000473:pathology*; D012867:Skin / Q000473:pathology
chemical_list: D007166:Immunosuppressive Agents; D008727:Methotrexate