The effects of Atm haploinsufficiency on mutation rate in the mouse germ line and somatic tissue.
Using single-molecule polymerase chain reaction, the frequency of spontaneous and radiation-induced mutation at an expanded simple tandem repeat (ESTR) locus was studied in DNA samples extracted from sperm and bone marrow of Atm knockout (Atm(+/-)) heterozygous male mice. The frequency of spontaneous mutation in sperm and bone marrow in Atm(+/-) males did not significantly differ from that in wild-type BALB/c mice. Acute exposure to 1 Gy of gamma-rays did not affect ESTR mutation frequency in bone marrow and resulted in similar increases in sperm samples taken from Atm(+/-) and BALB/c males. Taken together, these results suggest that the Atm haploinsufficiency analysed in our study does not affect spontaneous and radiation-induced ESTR mutation frequency in mice.
Ahuja
Akshay K
AK
Department of Genetics, University of Leicester, Leicester LE1 7RH, UK.
Barber
Ruth C
RC
Hardwick
Robert J
RJ
Weil
Michael M
MM
Genik
Paula C
PC
Brenner
David J
DJ
Dubrova
Yuri E
YE
D000818:Animals; D064007:Ataxia Telangiectasia Mutated Proteins; D001853:Bone Marrow / Q000528:radiation effects; D018797:Cell Cycle Proteins / Q000235:genetics / Q000502:physiology*; D004252:DNA Mutational Analysis; D042622:DNA Repeat Expansion / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000502:physiology*; D005720:Gamma Rays; D005854:Germ Cells / Q000528:radiation effects; D018095:Germ-Line Mutation; D006238:Haploidy; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000502:physiology*; D013094:Spermatozoa / Q000528:radiation effects; D025521:Tumor Suppressor Proteins / Q000235:genetics / Q000502:physiology*
D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D025521:Tumor Suppressor Proteins; D064007:Ataxia Telangiectasia Mutated Proteins; C576325:Atm protein, mouse; D017346:Protein Serine-Threonine Kinases