title: A dipeptide YY derived from royal jelly proteins inhibits renin activity.
abstract: Renin is the rate limiting enzyme in the renin-angiotensin (RA) system that regulates blood pressure and electrolyte balance. In this study, we investigated the renin inhibitory effect of a royal jelly (RJ)-derived peptide. A dipeptide YY was isolated from the digested fraction of RJ proteins by proteases and was found to inhibit human renin activity. The inhibition constant (Ki) of YY was estimated to be 10 microM when the Km was 0.16 microM using sheep angiotensinogen as the substrate. The peptide was observed to lower blood pressure in spontaneously hypertensive rats.
authors.affiliation: United Graduate School of Agricultural Science, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.
authors.forename: Afroza
authors.initials: A
authors.lastname: Sultana
authors.forename: A H M Nurun
authors.initials: AH
authors.lastname: Nabi
authors.forename: Uddin M
authors.initials: UM
authors.lastname: Nasir
authors.forename: Hiroe
authors.initials: H
authors.lastname: Maruyama
authors.forename: Kazu-Michi
authors.initials: KM
authors.lastname: Suzuki
authors.forename: Satoshi
authors.initials: S
authors.lastname: Mishima
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Suzuki
mesh_terms: D000808:Angiotensinogen / Q000235:genetics / Q000378:metabolism; D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D016466:CHO Cells; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D018076:DNA, Complementary / Q000235:genetics; D004151:Dipeptides / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D004797:Enzyme-Linked Immunosorbent Assay; D005227:Fatty Acids / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D005260:Female; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D006973:Hypertension / Q000503:physiopathology / Q000517:prevention & control; D019476:Insect Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D015394:Molecular Structure; D010447:Peptide Hydrolases / Q000378:metabolism; D051381:Rats; D011918:Rats, Inbred SHR; D011994:Recombinant Proteins / Q000378:metabolism; D012083:Renin / Q000235:genetics / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000187:drug effects; D012756:Sheep; D000099219:Royal Jelly
chemical_list: D000808:Angiotensinogen; D018076:DNA, Complementary; D004151:Dipeptides; D005227:Fatty Acids; D019476:Insect Proteins; D010447:Peptide Hydrolases; D011994:Recombinant Proteins; D012083:Renin; D000099219:Royal Jelly