Mesoporous silica nanoparticles improve magnetic labeling efficiency in human stem cells.
Tumblerlike magnetic/fluorescein isothiocyanate (FITC)-labeled mesoporous silica nanoparticles, Mag-Dye@MSNs, have been developed, which are composed of silica-coated core-shell superparamagnetic iron oxide (SPIO@SiO(2)) nanoparticles co-condensed with FITC-incorporated mesoporous silica. Mag-Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and in vivo, as manifested by using a clinical 1.5-T MRI system with requirements of simultaneous low incubation dosage of iron, low detection cell numbers, and short incubation time. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes, which can still be readily detected by MRI. Moreover, a higher MRI signal intensity decrease is observed in Mag-Dye@MSN-treated cells than in SPIO@SiO(2)-treated cells. This is the first report that MCM-41-type MSNs are advantageous to cellular uptake, as manifested by a higher labeling efficiency of Mag-Dye@MSNs than SPIO@SiO(2).
Liu
Hon-Man
HM
Department of Medical Imaging, National Taiwan University Hospital and College of Medicine National Taiwan University, Taipei, Taiwan.
Wu
Si-Han
SH
Lu
Chen-Wen
CW
Yao
Ming
M
Hsiao
Jong-Kai
JK
Hung
Yann
Y
Lin
Yu-Shen
YS
Mou
Chung-Yuan
CY
Yang
Chung-Shi
CS
Huang
Dong-Ming
DM
Chen
Yao-Chang
YC
D002478:Cells, Cultured; D003287:Contrast Media / Q000737:chemistry*; D003460:Crystallization / Q000379:methods; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry; D008280:Magnetics; D008422:Materials Testing; D008968:Molecular Conformation; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000379:methods*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods; D013234:Stem Cells / Q000166:cytology*; D013499:Surface Properties
D003287:Contrast Media; D004337:Drug Carriers; D046911:Macromolecular Substances; D012822:Silicon Dioxide