title: Oncotic colpocytology stained with Harris-Shorr in the observation of vaginal microorganisms.
abstract: The purpose of this work was to evaluate the efficacy of oncotic colpocytology stained with Harris-Shorr in the identification of the cervicovaginal microflora and infectious agents. Results of microbiologic evaluation carried out in colpocytology exams, bacterioscopy (Gram), and direct exams of 2,017 women aged from 13 to 80 years were compared. Of these, 83.1% agreed between cytology and Gram, 3.6% partially agreed, and 12.8% disagreed. The predominant microflora was of lactobacilli (63.3%), followed by mixed flora (32.1%). The results of sensitivity to lactobacilli were 96.1% and to mixed flora 88.0%; the specificity values were 91.2 and 92.0%, respectively. Colpocytology detected all the instances of trichomoniasis observed at direct exam (0.6%). The most frequent infectious agents were of candidiasis (14.8%, sensitivity 80.3%) and bacterial vaginosis (11.9%, sensitivity 68.1%). Thus, Harris-Shorr stained cytology was shown to be an excellent diagnostic method for T. vaginalis, lactobacilli, mixed flora, and candidiasis.
authors.affiliation: Division of Pathological Anatomy and Clinical Cytology, Souza Laboratory of Pathology, Maringá, Paraná, Brazil.
authors.forename: Agenor
authors.initials: A
authors.lastname: Storti-Filho
authors.forename: Terezinha Inez
authors.initials: TI
authors.lastname: Estivalet Svidizinski
authors.forename: Robson José
authors.initials: RJ
authors.lastname: da Silva Souza
authors.forename: Ione Cristina Jorge
authors.initials: IC
authors.lastname: de Mello
authors.forename: Paola
authors.initials: P
authors.lastname: da Costa Souza
authors.forename: Marcia Edilaine
authors.initials: ME
authors.lastname: Lopes Consolaro
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D002181:Candidiasis, Vulvovaginal / Q000175:diagnosis* / Q000382:microbiology; D005260:Female; D005840:Gentian Violet; D006801:Humans; D007778:Lactobacillus / Q000302:isolation & purification; D008875:Middle Aged; D010619:Phenazines; D012680:Sensitivity and Specificity; D013194:Staining and Labeling; D014247:Trichomonas Vaginitis / Q000175:diagnosis* / Q000382:microbiology; D014246:Trichomonas vaginalis / Q000302:isolation & purification; D014621:Vagina / Q000382:microbiology*; D014626:Vaginal Smears; D016585:Vaginosis, Bacterial / Q000175:diagnosis* / Q000382:microbiology
chemical_list: C035863:Gram's stain; D010619:Phenazines; D005840:Gentian Violet