Genome-wide survey of the RIP domain family in Oryza sativa and their expression profiles under various abiotic and biotic stresses.
Ribosome-inactivating proteins (RIPs) are N-glycosidases that inhibit protein synthesis by depurinating rRNA. Despite their identification more than 25 years ago, little is known about their biological functions. Here, we report a genome-wide identification of the RIP family in rice based on the complete genome sequence analysis. Our data show that rice genome encodes at least 31 members of this family and they all belong to type 1 RIP genes. This family might have evolved in parallel to species evolution and genome-wide duplications represent the major mechanism for this family expansion. Subsequently, we analyzed their expression under biotic (bacteria and fungus infection), abiotic (cold, drought and salinity) and the phytohormone ABA treatment. These data showed that some members of this family were expressed in various tissues with differentiated expression abundances whereas several members showed no expression under normal growth conditions or various environmental stresses. On the other hand, the expression of many RIP members was regulated by various abiotic and biotic stresses. All these data suggested that specific members of the RIP family in rice might play important roles in biotic and abiotic stress-related biological processes and function as a regulator of various environmental cues and hormone signaling. They may be potentially useful in improving plant tolerance to various abiotic and biotic stresses by over-expressing or suppressing these genes.
Jiang
Shu-Ye
SY
Rice Functional Genomics Group, Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore, 117604, Singapore.
Ramamoorthy
Rengasamy
R
Bhalla
Ritu
R
Luan
Hong-Fen
HF
Venkatesh
Prasanna Nori
PN
Cai
Minne
M
Ramachandran
Srinivasan
S
D000222:Adaptation, Physiological; D003681:Dehydration; D019143:Evolution, Molecular; D020440:Gene Duplication; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D018745:Genome, Plant; D005810:Multigene Family; D012275:Oryza / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D017434:Protein Structure, Tertiary; D054789:Ribosome Inactivating Proteins, Type 1 / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology
D054789:Ribosome Inactivating Proteins, Type 1; D012965:Sodium Chloride