title: Loss of GABAergic neurons in the subiculum and its functional implications in temporal lobe epilepsy.
abstract: Clinical and experimental evidence suggest that the subiculum plays an important role in the maintenance of temporal lobe seizures. Using the pilocarpine-model of temporal lobe epilepsy (TLE), the present study examines the vulnerability of GABAergic subicular interneurons to recurrent seizures and determines its functional implications. In the subiculum of pilocarpine-treated animals, the density of glutamic acid decarboxylase (GAD) mRNA-positive cells was reduced in all layers. Our data indicate a substantial loss of parvalbumin-immunoreactive neurons in the pyramidal cell and molecular layer whereas calretinin-immunoreactive cells were predominantly reduced in the molecular layer. Though the subiculum of pilocarpine-treated rats showed an increased intensity of GAD65 immunoreactivity, the density of GAD65 containing synaptic terminals in the pyramidal cell layer was decreased indicating an increase in the GAD65 intensity of surviving synaptic terminals. We observed a decrease in evoked inhibitory post-synaptic currents that mediate dendritic inhibition as well as a decline in the frequency of miniature inhibitory post-synaptic currents (mIPSCs) that are restricted to the perisomatic region. The decrease in mIPSC frequency (-30%) matched with the reduced number of perisomatic GAD-positive terminals (-28%) suggesting a decrease of pre-synaptic GABAergic input onto pyramidal cells in epileptic animals. Though cell loss in the subiculum has not been considered as a pathogenic factor in human and experimental TLE, our data suggest that the vulnerability of subicular GABAergic interneurons causes an input-specific disturbance of the subicular inhibitory system.
authors.affiliation: Dept. of Psychiatry and Psychotherapy, Charité-Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Knopp
authors.forename: Christiane
authors.initials: C
authors.lastname: Frahm
authors.forename: Pawel
authors.initials: P
authors.lastname: Fidzinski
authors.forename: Otto W
authors.initials: OW
authors.lastname: Witte
authors.forename: Joachim
authors.initials: J
authors.lastname: Behr
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D003712:Dendrites / Q000473:pathology; D004569:Electroencephalography; D004833:Epilepsy, Temporal Lobe / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D005968:Glutamate Decarboxylase / Q000032:analysis / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology; D007150:Immunohistochemistry; D017403:In Situ Hybridization / Q000379:methods; D007395:Interneurons / Q000378:metabolism / Q000473:pathology* / Q000502:physiology; D018408:Patch-Clamp Techniques; D010862:Pilocarpine; D017729:Presynaptic Terminals / Q000473:pathology; D017966:Pyramidal Cells / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D013194:Staining and Labeling; D005680:gamma-Aminobutyric Acid / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D010862:Pilocarpine; D005680:gamma-Aminobutyric Acid; D005968:Glutamate Decarboxylase; C401141:glutamate decarboxylase 2