The effects of thalidomide on chemotactic migration of multiple myeloma cell lines.
We examined the effect of thalidomide and dexamethasone on the migration of multiple myeloma (MM) cell lines, U266, RPMI8226, and NCI-H929, using chemotaxis chamber plates. U266 underwent chemotactic migration in response to stromal-cell derived factor-1 alpha (SDF-1alpha), and other cell lines underwent random migration in response to SDF-1alpha or monocyte chemotactic protein-1 alpha. Following preincubation with 1 mug/ml thalidomide, the cell lines showed reduced migratory capacity in response to SDF-1alpha. Concerning the corresponding receptors, CXC chemokine receptor 4 was detected only on the surface of U266, by flow cytometry, whereas chemokine (C-C motif) receptor 2 was not detected on all three cell lines. Moreover, decreased migration by thalidomide was not accompanied by altered expression of the corresponding receptors of each cell line. This is the first report to show the effects of thalidomide on the migration of MM cell lines. The results suggest that the inhibition of chemotactic migration might be one of the mechanisms of the success of thalidomide in controlling MM.
Fuchida
S-I
SI
Department of Medicine, Division of Hematology and Oncology, Kyoto Prefectural University of Medicine, Kyoto, Japan.
Shimazaki
C
C
Hirai
H
H
Akamatsu
S
S
Yamada
N
N
Uchida
R
R
Okano
A
A
Okamoto
M
M
Inaba
T
T
Taniwaki
M
M
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D054377:Chemokine CXCL12 / Q000187:drug effects; D002633:Chemotaxis / Q000187:drug effects*; D006801:Humans; D009101:Multiple Myeloma / Q000188:drug therapy; D010950:Plasma Cells / Q000187:drug effects*; D019707:Receptors, Chemokine / Q000187:drug effects; D013792:Thalidomide / Q000494:pharmacology*
D000970:Antineoplastic Agents; D054377:Chemokine CXCL12; D019707:Receptors, Chemokine; D013792:Thalidomide