title: Effects of fluoride on DNA damage, S-phase cell-cycle arrest and the expression of NF-kappaB in primary cultured rat hippocampal neurons.
abstract: The mechanisms underlying the neurotoxicity of fluorosis still remain obscure. To investigate DNA damage, cell-cycle distribution and expression of nuclear factor kappa B (NF-kappaB) induced by fluoride, the primary rat hippocampal neurons were incubated with various concentrations (20mg/l, 40 mg/l, and 80 mg/l) of sodium fluoride for 24 h in vitro. Our results showed that olive tail moments (OTMs) were significantly elevated in all fluoride-treated groups, while significant increases in the percentage of DNA in the tail were, respectively, observed at 40 mg/l and 80 mg/l levels of fluoride. An increase in the proportion of cells in S-phase was observed in response to the treatment of 40 mg/l and 80 mg/l fluoride. Gene expression of NF-kappaB was also enhanced by fluoride treatment in a dose-dependent manner. The results indicated that fluoride could induce S-phase cell-cycle arrest, up-regulation of NF-kappaB and DNA damage in primary rat hippocampal neurons.
authors.affiliation: MOE Key Lab of Environment and Health, Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, PR China.
authors.forename: Ming
authors.initials: M
authors.lastname: Zhang
authors.forename: Aiguo
authors.initials: A
authors.lastname: Wang
authors.forename: Tao
authors.initials: T
authors.lastname: Xia
authors.forename: Ping
authors.initials: P
authors.lastname: He
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D020552:Comet Assay; D004247:DNA / Q000187:drug effects*; D004249:DNA Damage; D004261:DNA Replication / Q000187:drug effects; D005333:Fetus / Q000166:cytology; D015870:Gene Expression; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D016196:S Phase / Q000187:drug effects*; D012969:Sodium Fluoride / Q000633:toxicity*
chemical_list: D016328:NF-kappa B; D012969:Sodium Fluoride; D004247:DNA