title: Autocrine motility factor stimulates the invasiveness of malignant cells as well as up-regulation of matrix metalloproteinase-3 expression via a MAPK pathway.
abstract: The autocrine motility factor (AMF) is a multifunctional protein that is involved in tumor progression including enhanced invasiveness via induction of matrix metalloproteinase-3 (MMP3). The increase in MMP3 was found in an AMF-high production tumor cell line, and c-Jun, c-Fos and mitogen-activated protein kinases (MAPKs) were also highly phosphorylated compared with the parent line. AMF stimulation induced the rapid phosphorylation of the cellular MAPK cascade and MMP3 secretion, which was blocked using a specific MAPK inhibitor. Results of this study suggest that AMF stimulation stimulates MMP3 expression via a MAPK signaling pathway.
authors.affiliation: Gifu Prefectural Research Institute for Health and Environmental Science, 1-1, Naka-Fudougaoka, Kakamigahara 504-0838, Japan. haga-arayo@pref.gifu.lg.jp <haga-arayo@pref.gifu.lg.jp>
authors.forename: Arayo
authors.initials: A
authors.lastname: Haga
authors.forename: Tatsuyoshi
authors.initials: T
authors.lastname: Funasaka
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Deyashiki
authors.forename: Avraham
authors.initials: A
authors.lastname: Raz
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D004789:Enzyme Activation; D005956:Glucose-6-Phosphate Isomerase / Q000378:metabolism* / Q000494:pharmacology; D006801:Humans; D048670:MAP Kinase Kinase 4 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019278:Matrix Metalloproteinase 3 / Q000235:genetics / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D060285:Receptors, Autocrine Motility Factor; D018121:Receptors, Cytokine / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D018121:Receptors, Cytokine; C555000:AMFR protein, human; D060285:Receptors, Autocrine Motility Factor; D044767:Ubiquitin-Protein Ligases; D020928:Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4; D019278:Matrix Metalloproteinase 3; D005956:Glucose-6-Phosphate Isomerase