Time-course of GDNF and its receptor expression after brain injury in the rat.
The aim of the present work was to perform, by in situ hybridization, a time-course analysis of the glial cell line-derived neurotrophic factor (GDNF) and its receptor mRNA expression in two models of brain injury in the rat: (a) excitotoxic lesion by ibotenic acid injection in the hippocampal formation; (b) mechanical lesion by needle insertion through the cerebral cortex including the white matter of the corpus callosum. The time-course analysis, ranging from 6h to 8 days, showed that the GDNF and its receptor (RET, GFRalpha-1 and GFRalpha-2) mRNA expressions were differentially up-regulated in both models of lesion. This in vivo regulation of the GDNF and its receptor mRNA expression indicates their involvement in the process of neuronal protection and regeneration occurring after brain injury.
Cheng
Qingzhang
Q
University of Palermo, Department of Experimental Medicine, Division of Human Physiology, corso Tukory 129, 1-90134 Palermo, Italy.
Di Liberto
Valentina
V
Caniglia
Giuseppa
G
Mudò
Giuseppa
G
D000818:Animals; D001345:Autoradiography / Q000379:methods; D001930:Brain Injuries / Q000139:chemically induced / Q000378:metabolism* / Q000503:physiopathology*; D005786:Gene Expression Regulation / Q000502:physiology*; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism*; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors / Q000235:genetics / Q000378:metabolism*; D007051:Ibotenic Acid; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D051100:Glial Cell Line-Derived Neurotrophic Factor; D051097:Glial Cell Line-Derived Neurotrophic Factor Receptors; D012333:RNA, Messenger; D007051:Ibotenic Acid