[Effects of podophyllotoxin solid lipid nanoparticles on proliferation and apoptosis of cervical carcinoma cells].
OBJECTIVE
To evaluate the anti-proliferative and apoptosis-inducing effect of podophyllotoxin solid lipid nanoparticles (PDP-SLN) in human cervical carcinoma cells in vitro.


METHODS
Hela cells were treated with PDP and PDP-SLN at different concentrations (0.0005-5 micromol/L), and the proliferation of the cells was assessed using MTT assay and the apoptotic index was determined by flow cytometry.


RESULTS
Both PDP and PDP-SLN showed obvious inhibitory effect on the cell proliferation in a dose- and time-dependent manner. At the same concentration, PDP-SLN produced stronger inhibitory effect on the cells than PDP, with IC50 24, 48, and 72 h after the cell exposure to PDP-SLN and PDP of 4.10, 0.65, 0.20 micromol/L and 9.2, 4.0, 1.3 micromol/L, respectively. Both PDP and PDP-SLN significantly induced the apoptosis of the Hela cell, and the apoptosis rates of the cells incubated in the presence of 0.5 micromol/L PDP-SLN reached 90.8% at 24 h and 94.2% at 72 h, significantly higher than the rate of cells incubated with PDP (64.1% at 24 h and 68.4% at 72 h, P<0.01).


CONCLUSIONS
PDP-SLN can effectively suppress the proliferation and induce apoptosis of Hela cells in vitro.
Shi
Yu-jie
YJ
Department of Dermatology Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. shiyujie-139@163.com
Zeng
Kang
K
Li
Guo-feng
GF
Zhang
Min
M
Zhu
Xiao-liang
XL
Sun
Le-dong
LD
Yang
Xi-xiao
XX
D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D005260:Female; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes; D053758:Nanoparticles / Q000737:chemistry*; D011034:Podophyllotoxin / Q000737:chemistry / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D008081:Liposomes; D011034:Podophyllotoxin