Separation of chitosan oligomers by immobilized metal affinity chromatography.
A novel approach for chitosan oligosaccharide (COS) separation by immobilized metal affinity chromatography (IMAC) based on the differences in the interactions of chelated copper (II) ions with various COS (dimers, trimers, tetramers) is described. Polyhydroxylic chromatographic supports (agarose CL-6B and silica) were functionalized with various chelating functions such as iminodiacetate (IDA), carboxymethyl-aspartate (CM-Asp) and tris(carboxymethyl)ethylenediamine (TED). The COS retention capacities of the columns were between 2 and 6 mg/cm(3), depending on the chelating group. The COS were separated and/or enriched up to 95% for dimer and trimer and 90% for the tetramer, with yields of 60-95%.
Le Dévédec
Frantz
F
Department of Chemistry & Center BioMed, Université du Québec à Montréal, CP 8888, Succursale Centre Ville Montréal (Québec), H3C 3P8, Canada.
Bazinet
Laurent
L
Furtos
Alexandra
A
Venne
Karine
K
Brunet
Serge
S
Mateescu
Mircea Alexandru
MA
D002614:Chelating Agents / Q000737:chemistry; D048271:Chitosan / Q000737:chemistry / Q000302:isolation & purification*; D002846:Chromatography, Affinity / Q000379:methods*; D002855:Chromatography, Thin Layer; D003300:Copper / Q000737:chemistry; D019281:Dimerization; D008670:Metals / Q000737:chemistry*; D015394:Molecular Structure; D017550:Spectroscopy, Fourier Transform Infrared
D002614:Chelating Agents; D008670:Metals; D003300:Copper; D048271:Chitosan