LMNA mRNA expression is altered in human obesity and type 2 diabetes.
OBJECTIVE
The aim of this study was to analyze lamin A/C mRNA levels in abdominal subcutaneous adipose tissue in two conditions-obesity and type 2 diabetes-that share common inflammatory and metabolic features, and to assess their relationship with selected inflammatory and adipogenic genes.


METHODS
This is a cross-sectional study involving 52 nondiabetic and 54 type 2 diabetes patients. Anthropometrical and analytical measurements (glycemic, lipidic, and inflammatory profiles) were performed, and mRNA expression was determined using real-time PCR.


RESULTS
Lamin A and C isoforms are expressed differentially. Lamin A/C mRNA levels were increased in obese and in type 2 diabetes patients. We also observed a strong relationship between both isoforms (B = 2.218, P < 0.001) and among lamin C mRNA expression and adipogenic (sterol-responsive element binding protein-1 (SREBP1c)) and inflammatory (interleukin-6 (IL-6)) markers (B = 0.854, P = 0.001, and B = 0.557, P < 0.001, respectively).


CONCLUSIONS
These data suggest that lamin A/C may be involved in the adipocyte gene profile observed in obesity and type 2 diabetes.
Miranda
Merce
M
Endocrinology and Diabetes Unit, Research Department, University Hospital of Tarragona Joan XXIII, Pere Virgili Institute, Rovira i Virgili University, Tarragona, Spain. mmiranda.hj23.ics@gencat.net
Chacón
Matilde R
MR
Gutiérrez
Cristina
C
Vilarrasa
Nuria
N
Gómez
Jose M
JM
Caubet
Enric
E
Megía
Ana
A
Vendrell
Joan
J
D000273:Adipose Tissue / Q000378:metabolism; D000328:Adult; D000368:Aged; D015331:Cohort Studies; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D005260:Female; D006706:Homeostasis; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism; D034904:Lamin Type A / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism*; D012044:Regression Analysis; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism
D015850:Interleukin-6; C494847:LMNA protein, human; D034904:Lamin Type A; D020033:Protein Isoforms; D012333:RNA, Messenger; D051780:Sterol Regulatory Element Binding Protein 1