Establishment of paternity testing system using microsatellite markers in Chinese Holstein.
To estimate the efficiency of microsatellite markers in paternity testing among Chinese Holstein, 30 microsatellite loci were used to differentiate 330 Chinese Holstein genotypes, according to the calculation of the allele frequency, number of alleles, effective number of alleles, genetic heterozygosity, polymorphic information content (PIC), and the exclusion probability in this cattle population. The results demonstrated that the exclusion probability ranged from 0.620 in locus BM1818 to 0.265 in locus INRA005 with the average of 0.472 and 11 microsatellite markers exceeding 0.5. The combined exclusion probability of nine microsatellite markers was over 0.99. The result showed that paternity testing of Chinese Holstein was basically resolved using the nine microsatellite markers selected.
Tian
Fei
F
College of Animal Science and Technology and Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, China Agricultural University, Beijing 100094, China.
Sun
Dongxiao
D
Zhang
Yuan
Y
D000483:Alleles; D000818:Animals; D002417:Cattle / Q000145:classification / Q000235:genetics*; D005220:Fathers; D005260:Female; D006579:Heterozygote; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D011110:Polymorphism, Genetic; D011336:Probability