title: Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis.
abstract: Karyotypes of species sects. Linum and Adenolinum have been studied using C/DAPI-banding, Ag-NOR staining, FISH with 5S and 26S rDNA and RAPD analysis. C/DAPI-banding patterns enabled identification of all homologous chromosome pairs in the studied karyotypes. The revealed high similarity between species L. grandiflorum (2n = 16) and L. decumbens by chromosome and molecular markers proved their close genome relationship and identified the chromosome number in L. decumbens as 2n = 16. The similarity found for C/DAPI-banding patterns between species with the same chromosome numbers corresponds with the results obtained by RAPD-analysis, showing clusterization of 16-, 18- and 30-chromosome species into three separate groups. 5S rDNA and 26S rDNA were co-localized in NOR-chromosome 1 in the genomes of all species investigated. In 30-chromosome species, there were three separate 5S rDNA sites in chromosomes 3, 8 and 13. In 16-chromosome species, a separate 5S rDNA site was also located in chromosome 3, whereas in 18-chromosome species it was found in the long arm of NOR-chromosome 1. Thus, the difference in localization of rDNA sites in species with 2n = 16, 2n = 30 and 2n = 18 confirms taxonomists opinion, who attributed these species to different sects. Linum and Adenolinum, respectively. The obtained results suggest that species with 2n = 16, 2n = 18 and 2n = 30 originated from a 16-chromosome ancestor.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia. omur@eimb.ru
authors.forename: Olga V
authors.initials: OV
authors.lastname: Muravenko
authors.forename: Olga Yu
authors.initials: OY
authors.lastname: Yurkevich
authors.forename: Nadezhda L
authors.initials: NL
authors.lastname: Bolsheva
authors.forename: Tatiana E
authors.initials: TE
authors.lastname: Samatadze
authors.forename: Inna V
authors.initials: IV
authors.lastname: Nosova
authors.forename: Daria A
authors.initials: DA
authors.lastname: Zelenina
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Volkov
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Popov
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Zelenin
mesh_terms: D002871:Chromosome Banding; D032461:Chromosomes, Plant / Q000235:genetics*; D020732:Cytogenetic Analysis; D018744:DNA, Plant / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics; D019597:Flax / Q000235:genetics*; D005819:Genetic Markers; D018745:Genome, Plant / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D010802:Phylogeny; D019105:Random Amplified Polymorphic DNA Technique
chemical_list: D018744:DNA, Plant; D004275:DNA, Ribosomal; D005819:Genetic Markers