Identification and allergenic characterisation of a new isoform of the A. simplex allergen Ani s 4.
Anisakis simplex hypersensitivity is a growing disease in developed countries. A positive diagnosis usually leads to the dietary recommendation of avoiding fish and seafood consumption. The protein Ani s 4 is a clinically relevant allergen due to its heat and pepsin resistant properties and its importance in the anaphylaxis process. The attempt of cloning Ani s 4 has led to the identification and characterisation of a new isoform that differs only in one amino acid with the previously published. This isoform was produced as an His tagged recombinant protein and its allergenic properties were tested by IgE immunoblot and by a flow cytometry basophil activation test. The results were compared to the allergenic properties of the isoform previously described. Both isoforms of Ani s 4 showed different capacities to bind IgE from sensitised patients and different potencies in the basophil activation test.
Rodriguez-Mahillo
Ana I
AI
Immunology Department, Hospital Carlos III, Sinesio Delgado 10, Madrid, Spain. anai_rm@yahoo.es
Gonzalez-Muñoz
Miguel
M
Moneo
Ignacio
I
D000485:Allergens / Q000276:immunology* / Q000302:isolation & purification; D000818:Animals; D017163:Anisakis / Q000276:immunology*; D000947:Antigens, Helminth / Q000276:immunology* / Q000302:isolation & purification; D001491:Basophils / Q000276:immunology; D003001:Cloning, Molecular; D019049:Developed Countries; D015801:Helminth Proteins / Q000276:immunology* / Q000302:isolation & purification; D006801:Humans; D007073:Immunoglobulin E / Q000378:metabolism; D011485:Protein Binding; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins / Q000276:immunology / Q000302:isolation & purification
D000485:Allergens; D000947:Antigens, Helminth; D015801:Helminth Proteins; D020033:Protein Isoforms; D011993:Recombinant Fusion Proteins; D007073:Immunoglobulin E