Bioavailability of water-soluble CoQ10 in beagle dogs.
The bioavailability of a novel water-soluble inclusion complex of CoQ10, prepared in our laboratory was determined and compared with the bioavailability of commercially available oil-based form of CoQ10. Experimental work consisted of single dose comparative bioavailability study on seven beagle dogs, with a 14-day washout period between treatments. Identification and quantification of CoQ10 was done with HPLC-MS method using positive APCI ionization and SIM mode, M+ m/z 863.4. The bioavailability results confirm that the water-soluble formulation has nearly three times higher AUC(0-48 h), two times higher Cmax, and Tmax is shortened from 6 to 4 h.
Prosek
Mirko
M
National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia.
Butinar
Janos
J
Lukanc
Barbara
B
Fir
Maja Milivojevic
MM
Milivojevic
Luka
L
Krizman
Mitja
M
Smidovnik
Andrej
A
D000042:Absorption; D000818:Animals; D019540:Area Under Curve; D001682:Biological Availability; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods / Q000662:veterinary; D018592:Cross-Over Studies; D004285:Dogs; D004355:Drug Stability; D006207:Half-Life; D013058:Mass Spectrometry / Q000379:methods / Q000662:veterinary; D008657:Metabolic Clearance Rate; D000432:Methanol / Q000737:chemistry; D008958:Models, Molecular; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D014815:Vitamins / Q000097:blood / Q000493:pharmacokinetics*; D014867:Water / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000378:metabolism
D014815:Vitamins; D047392:beta-Cyclodextrins; D014867:Water; D014451:Ubiquinone; C024989:coenzyme Q10; C031215:betadex; D000432:Methanol