In vitro and clinical evaluation of specific dentifrices for complete denture hygiene.
OBJECTIVE
To study the physical properties of two experimental dentifrices for complete denture hygiene, their effect on denture biofilm removal and antimicrobial properties by means of a clinical trial.


METHODS
The experimental dentifrices comprised two compositions. One was based on the addition of 1% chloramine T (D1) and the other on the presence of 0.01% fluorosurfactant (D2). Measurements of density, pH, consistency, rheological features and abrasiveness were conducted. Sixty complete denture wearers were randomly assigned to three groups and were instructed to brush their dentures with a specific toothbrush: (1) Water (control); (2) D1; or (3) D2. Each method was used for 21 days. Denture biofilm was disclosed by a 1% neutral red solution and quantified by means of digital photos taken from the internal surface. Microbiological assessment was conducted to quantify Candida sp. and mutans streptococci. Data were evaluated by one-way anova and Tukey HSD, or Kruskal-Wallis (alpha = 0.05).


RESULTS
Both dentifrices decreased biofilm coverage when compared with the control group. D1 was the most efficacious treatment to reduce mutans streptococci, whereas D2 showed an intermediate outcome (ANOVA, p < 0.040). No treatment influenced Candida albicans or non-albicans species (Kruskal-Wallis, p = 0.163 and 0.746, respectively).


CONCLUSIONS
It can be concluded that brushing complete dentures with the experimental dentifrices tested could be effective for the removal of denture biofilm.
Panzeri
Heitor
H
Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil.
Lara
Elza Helena Guimarães
EH
Paranhos
Helena de Freitas Oliveira
Hde F
Lovato da Silva
Claudia Helena
CH
de Souza
Raphael Freitas
RF
de Souza Gugelmin
Maria Cristina Monteiro
MC
Tirapelli
Camila
C
Cruz
Patrícia Costa
PC
de Andrade
Ingrid Machado
IM
D000368:Aged; D000890:Anti-Infective Agents / Q000627:therapeutic use; D000891:Anti-Infective Agents, Local / Q000627:therapeutic use; D018441:Biofilms / Q000187:drug effects; D002175:Candida / Q000187:drug effects / Q000302:isolation & purification; D002700:Chloramines / Q000627:therapeutic use; D004396:Coloring Agents; D003802:Dentifrices / Q000627:therapeutic use*; D003820:Denture Cleansers / Q000627:therapeutic use*; D003824:Denture, Complete / Q000382:microbiology; D005260:Female; D005459:Fluorides / Q000627:therapeutic use; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008422:Materials Testing; D008875:Middle Aged; D009499:Neutral Red; D009910:Oral Hygiene; D009930:Organic Chemicals / Q000627:therapeutic use; D012212:Rheology; D013295:Streptococcus mutans / Q000187:drug effects / Q000302:isolation & purification; D013501:Surface-Active Agents / Q000627:therapeutic use; D014099:Toothbrushing / Q000295:instrumentation; D014105:Tosyl Compounds / Q000627:therapeutic use; D016896:Treatment Outcome; D014783:Viscosity
D000890:Anti-Infective Agents; D000891:Anti-Infective Agents, Local; D002700:Chloramines; D004396:Coloring Agents; D003802:Dentifrices; D003820:Denture Cleansers; D009930:Organic Chemicals; D013501:Surface-Active Agents; D014105:Tosyl Compounds; C016300:chloramine-T; D009499:Neutral Red; C038646:Zonyl; D005459:Fluorides