A gel-forming beta-glucan isolated from the fruit bodies of the edible mushroom Pleurotus florida.
Glucans of basidiomycetes are considered to be an important class of polysaccharides, as they can act as biological response modifiers. We now isolate a gel-forming, water-soluble beta-glucan, with a molecular mass of 1.2 x 10(6)g/mol (HPSEC), from the fruit bodies of the edible mushroom Pleurotus florida, via alkaline extraction, followed by fractionation by freeze-thawing. Structural assignments were carried out using mono- and bi-dimensional nuclear magnetic resonance spectroscopy, monosaccharide composition, methylation analyses, and a controlled Smith degradation. It was a branched beta-glucan, with a main chain of (1-->3)-linked-Glcp residues, substituted at O-6 by single-unit Glcp side chains, on average to every fourth residue of the backbone.
Santos-Neves
Juliana C
JC
Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, CEP 81531-980 Curitiba, PR, Brazil.
Pereira
Maria Izabel
MI
Carbonero
Elaine R
ER
Gracher
Ana Helena P
AH
Gorin
Philip A J
PA
Sassaki
Guilherme L
GL
Iacomini
Marcello
M
D002240:Carbohydrate Sequence; D048690:Fruiting Bodies, Fungal / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D015394:Molecular Structure; D020076:Pleurotus / Q000378:metabolism*; D047071:beta-Glucans / Q000737:chemistry* / Q000302:isolation & purification
D047071:beta-Glucans