Characterisation and controlled drug release from novel drug-loaded hydrogels.
Hydrogel based devices belong to the group of swelling controlled drug delivery systems. Temperature responsive poly(N-isopropylacrylamide)-poly(vinylpyrrolidinone) random copolymers were produced by free radical polymerisation, using 1-hydroxycyclohexylphenyketone as an ultraviolet-light sensitive initiator, and poly(ethylene glycol) dimethacrylate as the crosslinking agent (where appropriate). The hydrogels were synthesised to have lower critical solution temperatures (LCST) near body temperature, which is favourable particularly for 'smart' drug delivery applications. Two model drugs (diclofenac sodium and procaine HCl) were entrapped within these xerogels, by incorporating the active agents prior to photopolymerisation. The properties of the placebo samples were contrasted with the drug-loaded copolymers at low levels of drug integration. Modulated differential scanning calorimetry (MDSC), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) and atomic force microscopy (AFM) were used to investigate the influence of the drugs incorporated on the solid-state properties of the xerogels. MDSC and swelling studies were carried out to ascertain their effects on the LCST and swelling behaviour of the hydrated samples. In all cases, drug dissolution analysis showed that the active agent was released at a slower rate at temperatures above the phase transition temperature. Finally, preliminary in vitro cytotoxicity evaluations were performed to establish the toxicological pattern of the gels.
Geever
Luke M
LM
Centre for Biopolymer and Biomolecular Research, Athlone Institute of Technology, C. Westmeath, Ireland.
Cooney
Ciaran C
CC
Lyons
John G
JG
Kennedy
James E
JE
Nugent
Michael J D
MJ
Devery
Sinead
S
Higginbotham
Clement L
CL
D000178:Acrylamides; D000180:Acrylic Resins; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations / Q000737:chemistry*; D004008:Diclofenac / Q000008:administration & dosage / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry* / Q000633:toxicity; D011108:Polymers; D011205:Povidone; D011343:Procaine / Q000008:administration & dosage / Q000737:chemistry; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D013778:Tetrazolium Salts; D013844:Thiazoles; D014466:Ultraviolet Rays
D000178:Acrylamides; D000180:Acrylic Resins; D000894:Anti-Inflammatory Agents, Non-Steroidal; D003432:Cross-Linking Reagents; D003692:Delayed-Action Preparations; D020100:Hydrogels; D011108:Polymers; D013778:Tetrazolium Salts; D013844:Thiazoles; D004008:Diclofenac; C052970:poly-N-isopropylacrylamide; D011343:Procaine; C022616:thiazolyl blue; D011205:Povidone