Benzene-di-N-substituted carbamates as conformationally constrained substrate analogs of cholesterol esterase.
Benzene-1,2-, 1,3-, and 1,4-di-N-substituted carbamates (1-15) are synthesized as the constrained analogs of gauche, eclipsed, and anti conformations, respectively, for the glycerol backbones of triacylglycerol. Carbamates 1-15 are characterized as the pseudo substrate inhibitors of cholesterol esterase. Long chain carbamates are more potent inhibitors than short chain ones. Comparison of different geometries for benzene-di-substituted carbamates, such as benzene-1,2-di-N-octylcarbamate (3) (ortho-3), benzene-1,3-di-N-octylcarbamate (8) (meta-8), and benzene-1,4-di-N-octylcarbamates (13) (para-13), indicates that inhibitory potencies are as followed: meta-8 > para-13 > ortho-3. Therefore, we suggest that the preferable conformation for the C(sn-1)-O/C(sn-2)-O glycerol backbone in the enzyme-triacylgycerol complex is the eclipsed conformation. Meanwhile, kinetic data indicate that among ortho, meta, and para carbamates, meta carbamates most resemble the substrate cholesterol ester.
Chiou
S-Y
SY
Division of Neurosurgery, Chung Shan Medical University Hospital, Taichung 402, Taiwan.
Lin
M-C
MC
Hwang
M-T
MT
Chang
H-G
HG
Lin
G
G
D001554:Benzene / Q000737:chemistry*; D002219:Carbamates / Q000737:chemistry* / Q000378:metabolism*; D008968:Molecular Conformation; D002787:Sterol Esterase / Q000378:metabolism*; D013379:Substrate Specificity
D002219:Carbamates; D002787:Sterol Esterase; D001554:Benzene