Expression and enzyme activity of alpha(1,6)fucosyltransferase in human colorectal cancer.
Changes in enzyme activity and the expression levels of alpha(1,6)fucosyltransferase [alpha(1,6)FT] have been reported in certain types of malignant transformations. To develop a better understanding of the role of alpha(1,6)FT in human colorectal carcinoma (CRC), we analysed the enzyme activity in healthy and tumour tissues. alpha(1,6)FT activity was considerably higher in tumour tissue than in healthy tissue and was related to gender, lymph node metastasis, type of growth and tumour stage. We also observed a significant increase in the alpha(1,6)FT expression in tumour tissues as compared to healthy and transitional tissues, inflammatory lesions and adenomas. The immunohistochemical expression in tumour tissues was correlated with the degree of infiltration through the intestinal wall. Finally, a statistical correlation was found between enzyme activity and expression obtained by Western blot in colorectal tumours when compared in the same patient. All these findings demonstrate an alteration of alpha(1,6)FT activity and expression in CRC.
Muinelo-Romay
Laura
L
Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, Vigo, Spain.
Vázquez-Martín
Cristina
C
Villar-Portela
Susana
S
Cuevas
Elisa
E
Gil-Martín
Emilio
E
Fernández-Briera
Almudena
A
D000230:Adenocarcinoma / Q000201:enzymology*; D000236:Adenoma / Q000201:enzymology; D000368:Aged; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002471:Cell Transformation, Neoplastic; D015179:Colorectal Neoplasms / Q000201:enzymology*; D005260:Female; D005647:Fucosyltransferases / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male
D014408:Biomarkers, Tumor; D005647:Fucosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase