title: 5-Methoxy-8-(2-hydroxy-3-buthoxy-3-methylbutyloxy)-psoralen isolated from Angelica dahurica inhibits cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells.
abstract: 5-Methoxy-8-(2-hydroxy-3-buthoxy-3-methylbutyloxy)-psoralen (MP) is a medicinal herbal product isolated from Angelica dahurica that inhibits the cyclooxygenase-2 (COX-2)-dependent phase of prostaglandin D(2) (PGD(2)) generation in bone marrow-derived mast cells (IC(50), 23.5 microM). Western blotting with specific anti-COX-2 antibodies showed that the decrease in PGD(2) production was accompanied by a decrease in COX-2 protein levels. In addition, this compound consistently inhibited the production of leukotriene C(4) in a dose dependent manner, (IC(50), 2.5 microM). These results demonstrate that MP inhibits both cyclooxygenase-2 and 5-lipoxygenase activity. Furthermore, this compound also inhibited the degranulation reaction (IC(50), 4.1 microM). Therefore, this compound might provide a basis for novel anti-inflammatory drug development.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyongsan, 712-749, Korea.
authors.forename: Jin Mei
authors.initials: JM
authors.lastname: Hua
authors.forename: Tae Chul
authors.initials: TC
authors.lastname: Moon
authors.forename: Tae Gyun
authors.initials: TG
authors.lastname: Hong
authors.forename: Kyong Min
authors.initials: KM
authors.lastname: Park
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
mesh_terms: D029969:Angelica; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000201:enzymology; D002478:Cells, Cultured; D052246:Cyclooxygenase 2 Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D011564:Furocoumarins / Q000302:isolation & purification* / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: C530809:5-methoxy-8-(2-hydroxy-3-butoxy-3-methylbutyloxy)psoralen; D000894:Anti-Inflammatory Agents, Non-Steroidal; D052246:Cyclooxygenase 2 Inhibitors; D011564:Furocoumarins; D016859:Lipoxygenase Inhibitors