Potential role of N-cadherin in hepatocyte growth factor (HGF) mediated improvement of the cardiac function of dilated cardiomyopathy mice.
OBJECTIVE
To study the potential mechanisms of hepatocyte growth factor (HGF) mediating improvement of the cardiac function of dilated cardiomyopathy mice (DCM).


RESULTS
We established experimental model of dilated cardiomyopathy by repetitive coxsickievirus B3 (CVB3) infection in Balb/c mice and half of the experimental group mice were injected 0.2 microg recombinated human HGF through tail vein every 2 days starting from the 5th month. At the 5th month, dilated cardiomyopathy occurred in experimental group mice. The HGF level and N-cadherin expression in myocardium of experimental group was downregulated. At the 7th month, after HGF supplement, the HGF level and N-cadherin expression in the myocardium of the mice were increased, the fibrosis of myocardial and cells apoptosis were ameliorated and cardiac function was improved. In vitro experiment, we found that N-cadherin expression was increased in cultured myocardial cells treated with rHGF.


CONCLUSIONS
Increasing in N-cadherin expression may be one of the potential mechanisms of HGF mediating Improvement of cardiac function of dilated cardiomyopathy mice.
Tang
Qizhu
Q
Shen
Difei
D
Huang
Zhengrong
Z
Xiong
Ran
R
Wu
Hui
H
Huang
Ji
J
Feng
Siting
S
Niu
Ping
P
Yang
Lin
L
Bian
Zhouyan
Z
D000818:Animals; D016275:Atrial Function / Q000187:drug effects / Q000502:physiology; D015820:Cadherins / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000502:physiology*; D002311:Cardiomyopathy, Dilated / Q000188:drug therapy / Q000503:physiopathology*; D002478:Cells, Cultured; D017228:Hepatocyte Growth Factor / Q000008:administration & dosage / Q000627:therapeutic use*; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000627:therapeutic use; D016276:Ventricular Function / Q000187:drug effects / Q000502:physiology
D015820:Cadherins; C066855:HGF protein, human; D011994:Recombinant Proteins; D017228:Hepatocyte Growth Factor