title: Immunohistochemical localization of transient receptor potential vanilloid subtype 1 in the trachea of ovalbumin-sensitized Guinea pigs.
abstract: BACKGROUND
  We previously found many transient receptor potential vanilloid receptor subtype 1 (TRPV1) axons in the tracheal smooth muscle and epithelium of the guinea pig airway. One report indicates that the number of TRPV1 axons is significantly increased in patients with cough variant asthma.
  
  
  OBJECTIVE
  To determine whether the distribution of TRPV1 in the airways is altered in guinea pigs with an allergic phenotype.
  
  
  METHODS
  Ten guinea pigs were assigned to 2 groups in a double-blind study. Five animals were sensitized with ovalbumin and the other 5 underwent sham sensitization. Cryopreserved sections (30 microm) of tracheal tissues removed from each animal were stained with polyclonal serum rabbit anti-TRPV1 antibody (1:30,000) and examined by confocal microscopy.
  
  
  RESULTS
  Axons immunoreactive to TRPV1 localized to fine axons within the epithelium and around areas of smooth muscle, were more densely stained and frequent in the ovalbumin than in the sham group.
  
  
  CONCLUSIONS
  The number of TRPV1-immunoreactive axons in the trachea increases under allergic inflammatory conditions.
authors.affiliation: Department of Respiratory and Infectious Diseases, St. Marianna University School of Medicine, Kawasaki, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Watanabe
authors.forename: Syunji
authors.initials: S
authors.lastname: Horie
authors.forename: Domenico
authors.initials: D
authors.lastname: Spina
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Michael
authors.forename: Clive P
authors.initials: CP
authors.lastname: Page
authors.forename: John V
authors.initials: JV
authors.lastname: Priestley
mesh_terms: D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000473:pathology; D001369:Axons / Q000737:chemistry / Q000276:immunology; D004195:Disease Models, Animal; D004311:Double-Blind Method; D006168:Guinea Pigs; D007150:Immunohistochemistry; D008297:Male; D018613:Microscopy, Confocal; D010047:Ovalbumin / Q000276:immunology; D050916:TRPV Cation Channels / Q000032:analysis / Q000096:biosynthesis*; D014132:Trachea / Q000276:immunology* / Q000294:innervation / Q000473:pathology
chemical_list: D050916:TRPV Cation Channels; C494484:TRPV1 receptor; D010047:Ovalbumin