title: Modified caspase-3 assay indicates correlation of caspase-3 activity with immunity of nonhuman primates to Yersinia pestis infection.
abstract: Defined candidate human vaccines for treating infection by Yersinia pestis, the agent of plague, have been developed. To facilitate evaluation of the vaccines' efficacy, the in vitro caspase-3 assay for cytotoxicity-neutralizing activity was modified and reevaluated. Immune serum-mediated caspase-3 neutralizing activity correlated with protection against infection in a nonhuman primate vaccine model of plague immunity.
authors.affiliation: Bacteriology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702, USA. susan.welkos@amedd.army.mil
authors.forename: Susan
authors.initials: S
authors.lastname: Welkos
authors.forename: Sarah
authors.initials: S
authors.lastname: Norris
authors.forename: Jeffrey
authors.initials: J
authors.lastname: Adamovicz
mesh_terms: D000818:Animals; D053148:Caspase 3 / Q000378:metabolism*; D004195:Disease Models, Animal; D007109:Immunity; D007118:Immunoassay / Q000379:methods*; D008252:Macaca fascicularis; D008264:Macrophages / Q000201:enzymology / Q000276:immunology / Q000382:microbiology; D009500:Neutralization Tests; D010930:Plague / Q000276:immunology* / Q000382:microbiology; D010931:Plague Vaccine / Q000276:immunology*; D015010:Yersinia pestis / Q000276:immunology*
chemical_list: D010931:Plague Vaccine; D053148:Caspase 3