Feasibility of using a surface plasmon resonance-based biosensor to detect and quantify yessotoxin.
Yessotoxin (YTX) is a disulfated polyether toxin produced by marine dinoflagellates. Although there is no clear evidence that YTX is toxic to humans, it is a major cause of false positives in DSP toxin detection by mouse bioassay. We developed a new detection and quantification method for yessotoxin using a BiaCore X Surface plasmon resonance (SPR)-based biosensor. The assay is based in the interaction of YTX with phosphodiesterase enzymes (PDE), one of its cellular targets. The injection of several YTX concentrations (3-12 microM) over immobilized PDE I, showed a dose dependent binding signal, which K(obs) (observed rate constant) allowed us to obtain a calibration curve with a linear fit. The detection of yessotoxin using SPR-based biosensor allows the quantification of the toxin with an automated and repetitive method at concentrations in the range of the 1 mg kg(-1) European regulatory limit.
Fonfría
Eva S
ES
Departamento de Farmacología, Facultad de Veterinaria, Universidad de Santiago de Compostela, Campus Universitario, 27002 Lugo, Spain.
Vilariño
Natalia
N
Vieytes
Mercedes R
MR
Yasumoto
Takeshi
T
Botana
Luis M
LM
D015374:Biosensing Techniques / Q000379:methods*; D004988:Ethers, Cyclic / Q000032:analysis* / Q000737:chemistry; D005240:Feasibility Studies; D015394:Molecular Structure; D008978:Mollusk Venoms; D035964:Oxocins / Q000032:analysis* / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods*
D004988:Ethers, Cyclic; D008978:Mollusk Venoms; D035964:Oxocins; C066632:yessotoxin