title: Development of a sensitive flow-injection-chemiluminescence detection method for the determination of levodopa.
abstract: A simple chemiluminometric method using flow injection has been developed for the determination of levodopa, based on its sensitizing effect on the weak chemiluminescence (CL) reaction between Na(2)SO(3) and acidic KMnO4. Under optimum experimental conditions, the CL intensity was linearly related to the concentration of levodopa from 3.4 x 10(-8) to 2.4 x 10(-5) mol/L and the detection limit was 1.1 x 10(-8) mol/L (s:n = 3). The relative standard deviation (RSD) of the proposed method calculated from 20 replicate injection of 3 x 10(-7) mol/L levodopa was 3.3%. The correlation coefficient was 0.997. The method was successfully applied to the determination of levodopa in commercial pharmaceutical formulations and spiked urine samples.
authors.affiliation: Department of Chemistry, Kyungpook National University, Taegu 702-701, Republic of Korea.
authors.forename: Seikh Mafiz
authors.initials: SM
authors.lastname: Alam
authors.forename: Mohammad Mainul
authors.initials: MM
authors.lastname: Karim
authors.forename: Sang Hak
authors.initials: SH
authors.lastname: Lee
authors.forename: Saikh Mohammad
authors.initials: SM
authors.lastname: Wabaidur
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Chung
authors.forename: Jong Ha
authors.initials: JH
authors.lastname: Choi
authors.forename: Meea
authors.initials: M
authors.lastname: Kang
mesh_terms: D017022:Flow Injection Analysis / Q000379:methods; D007980:Levodopa / Q000032:analysis* / Q000652:urine; D008163:Luminescent Measurements / Q000379:methods*; D008722:Methods; D004364:Pharmaceutical Preparations / Q000032:analysis; D011196:Potassium Permanganate; D013447:Sulfites
chemical_list: D004364:Pharmaceutical Preparations; D013447:Sulfites; D011196:Potassium Permanganate; D007980:Levodopa