title: Glucose transporter mediation responsible for morphological changes of human epithelial cells on glucose-displayed surfaces.
abstract: Cellular morphology is one of the important factors for coordinating cell signaling. In this study, the morphological variation via glucose transporter (GLUT)-mediated anchoring was investigated in the cultures of human mammary epithelial cells in the presence or absence of insulin on culture surfaces with the changed ratios of d- and l-glucose displayed. With increasing ratio of d-glucose displayed on the surfaces, the cells showed a stretched shape in the culture with 10 mug/cm(3) insulin, reaching the highest extent of cell stretching at 100%d-glucose display, whereas round cells were predominant at 0%d-glucose display. In the absence of insulin, on the other hand, the extent of cell stretching showed a concave profile in terms of the ratio of d-glucose displayed, the extent being highest at 50%d-glucose display. Blocking of integrin alpha(5)beta(1) or GLUTs1 and 4 on the cells using corresponding antibodies revealed that the primary mechanism for cell attachment was based on integrin-mediated binding, and that GLUTs1 and 4 contributed largely to morphological changes of cells. Confocal microscopy further revealed that GLUT4 localization occurred in response to d-glucose display as well as insulin addition. In the absence of insulin, GLUT4 spots were extensively observed in the cell body regardless of whether d-glucose was displayed or not. However, in the presence of insulin, the broad distribution of GLUT4 appeared on the basal and apical sides of cells at 100%d-glucose display, in contrast with its localization only on the apical side of cells at 0%d-glucose display. These results suggest that the quantitative balance between GLUTs on the cytoplasmic membrane and d-glucose displayed on a culture surface determines the cell morphology, as explained by the receptor saturation model.
authors.affiliation: Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
authors.forename: Mee-Hae
authors.initials: MH
authors.lastname: Kim
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kino-Oka
authors.forename: Masaya
authors.initials: M
authors.lastname: Kawase
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Yagi
authors.forename: Masahito
authors.initials: M
authors.lastname: Taya
mesh_terms: D000906:Antibodies / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology; D002462:Cell Membrane / Q000378:metabolism; D048430:Cell Shape / Q000187:drug effects / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D051272:Glucose Transporter Type 1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051275:Glucose Transporter Type 4 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006801:Humans; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D039081:Integrin alpha5beta1 / Q000037:antagonists & inhibitors / Q000378:metabolism; D042361:Mammary Glands, Human / Q000166:cytology / Q000378:metabolism*; D013499:Surface Properties; D013549:Sweetening Agents / Q000494:pharmacology
chemical_list: D000906:Antibodies; D051272:Glucose Transporter Type 1; D051275:Glucose Transporter Type 4; D007004:Hypoglycemic Agents; D007328:Insulin; D039081:Integrin alpha5beta1; C495677:SLC2A1 protein, human; C495692:SLC2A4 protein, human; D013549:Sweetening Agents; D005947:Glucose