title: High level of conservation between genes coding for the GAMYB transcription factor in barley (Hordeum vulgare L.) and bread wheat (Triticum aestivum L.) collections.
abstract: The transcription factor GAMYB is involved in gibberellin signalling in cereal aleurone cells and in plant developmental processes. Nucleotide diversity of HvGAMYB and TaGAMYB was investigated in 155 barley (Hordeum vulgare) and 42 wheat (Triticum aestivum) accessions, respectively. Polymorphisms defined 18 haplotypes in the barley collection and 1, 7 and 3 haplotypes for the A, B, and D genomes of wheat, respectively. We found that (1) Hv- and TaGAMYB genes have identical structures. (2) Both genes show a high level of nucleotide identity (>95%) in the coding sequences and the distribution of polymorphisms is similar in both collections. At the protein level the functional domain is identical in both species. (3) GAMYB genes map to a syntenic position on chromosome 3. GAMYB genes are different in both collections with respect to the Tajima D statistic and linkage disequilibrium (LD). A moderate level of LD was observed in the barley collection. In wheat, LD is absolute between polymorphic sites, mostly located in the first intron, while it decays within the gene. Differences in Tajima D values might be due to a lower selection pressure on HvGAMYB, compared to its wheat orthologue. Altogether our results provide evidence that there have been only few evolutionary changes in Hv- and TaGAMYB. This confirms the close relationship between these species and also highlights the functional importance of this transcription factor.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research Gatersleben (IPK), Corrensstrasse 3, Gatersleben, Germany. grit.haseneyer@wzw.tum.de
authors.forename: Grit
authors.initials: G
authors.lastname: Haseneyer
authors.forename: Catherine
authors.initials: C
authors.lastname: Ravel
authors.forename: Mireille
authors.initials: M
authors.lastname: Dardevet
authors.forename: François
authors.initials: F
authors.lastname: Balfourier
authors.forename: Pierre
authors.initials: P
authors.lastname: Sourdille
authors.forename: Gilles
authors.initials: G
authors.lastname: Charmet
authors.forename: Dominique
authors.initials: D
authors.lastname: Brunel
authors.forename: Sascha
authors.initials: S
authors.lastname: Sauer
authors.forename: Hartwig H
authors.initials: HH
authors.lastname: Geiger
authors.forename: Andreas
authors.initials: A
authors.lastname: Graner
authors.forename: Silke
authors.initials: S
authors.lastname: Stracke
mesh_terms: D001939:Bread; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D017124:Conserved Sequence; D017343:Genes, Plant; D014644:Genetic Variation; D006239:Haplotypes; D001467:Hordeum / Q000235:genetics*; D015810:Linkage Disequilibrium / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014157:Transcription Factors / Q000235:genetics*; D014908:Triticum / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors