[Effects of Newcastle disease virus on the expression of survivin and cell cycle in human tongue squamous carcinoma TSCCa cells].
OBJECTIVE
To investigate the effects of Newcastle disease virus (NDV) infection on the expression of survivin and cell cycle in human tongue squamous carcinoma TSCCa cells.


METHODS
The proliferation of TSCCa cells infected with NDV in vitro was evaluated by means of MTT assay, and survivin expression in the infected cells was detected using RT-PCR and Western blotting. Flow cytometry was performed to assess the changes in the cell apoptosis, cell cycle and cell proliferation index (PI) of the cells.


RESULTS
NDV infection resulted in decreased survivin expression and increased apoptosis of TSCCa cells, with reduced cell percentage in G2/M and S phases and lowered PI of the cells, showing significant differences from those of the negative control cells (P<0.05).


CONCLUSIONS
NDV infection can inhibit survivin expression, affect the cell cycle of TSCCa cells and induce their apoptosis.
Wan
Xue-qin
XQ
Southern Medical University, Guangzhou 510515, China. wanxq10@hotmail.com
Dai
Guo-yi
GY
Dou
Chang-wu
CW
Tian
Fu-ming
FM
Long
Min
M
Long
Bei-guo
BG
Wang
Xiao-juan
XJ
D017209:Apoptosis / Q000502:physiology*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology / Q000821:virology; D002453:Cell Cycle / Q000502:physiology*; D045744:Cell Line, Tumor; D054884:Host-Pathogen Interactions; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis* / Q000235:genetics; D009522:Newcastle disease virus / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077022:Survivin; D014062:Tongue Neoplasms / Q000378:metabolism / Q000473:pathology / Q000821:virology
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D000077022:Survivin