Toward high-throughput monitoring of metallodrug-protein interaction using capillary electrophoresis in chemically modified capillaries.
The performance of capillary electrophoresis (CE) operating with a sulfonated capillary for the separation of protein adducts of anticancer ruthenium(III)-based drugs was evaluated. The coated capillary was shown to yield improved resolution of albumin- and transferrin-bound species of ruthenium compared with that attained with the bare fused-silica capillary. The coating also showed an increased reproducibility of migration times and peak areas and allowed reasonably high efficiency separation of analytes (up to 1300 theoretical plates per meter), which display high affinity toward a fused-silica surface. In addition, due to rather high electroosmotic flow (EOF, > 45 x 10(-5)cm(2)V(-1)s(-1)) in the coated capillary, it enabled fast counter-EOF monitoring of albumin and transferrin adducts. This benefit, together with requiring only a short flush with the background electrolyte to have migration times reproducible (at < 1.5% relative standard deviation), makes this wall-modified capillary holding promise for CE examination of fast reactions such as those accompanying protein-drug interactions and biotransformations associated with drug delivery via protein binding.
Shmykov
Alexei Y
AY
Institute of Analytical Instrumentation, Russian Academy of Sciences, 190103 St. Petersburg, Russia.
Filippov
Vladimir N
VN
Foteeva
Lidia S
LS
Keppler
Bernhard K
BK
Timerbaev
Andrei R
AR
D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology*; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry*; D012428:Ruthenium / Q000737:chemistry* / Q000494:pharmacology*; D013997:Time Factors
D000970:Antineoplastic Agents; D011506:Proteins; D012428:Ruthenium