Ultra-sensitive detection of bacterial toxin with silicon nanowire transistor.
Nanowire field effect transistors (nano-FET) were lithographically fabricated using 50 nm doped polysilicon nanowires attached to two small gold terminals separated from each other by a approximately 150 nm gap to serve as the basis for electronic detection of bacteria toxins. The device characterizations, semiconducting properties and use in a robust and sensitive bio-molecular detection sensor of bacterial toxins were reported in this work. The device characteristics were demonstrated with varying gate and drain voltages. The bio-molecular detection was demonstrated using electrochemical impedance spectroscopy (EIS), using Staphylococcus aureus Enterotoxin B (SEB) as the target molecule. The detection limit of SEB was observed in the range of 10-35 fM.
Mishra
Nirankar N
NN
Center for Advanced Microelectronics and Biomolecular Research, University of Idaho, Post Falls, ID 83854, USA. nmishra@cambr.uidaho.edu
Maki
Wusi C
WC
Cameron
Eric
E
Nelson
Ron
R
Winterrowd
Paul
P
Rastogi
Shiva K
SK
Filanoski
Brian
B
Maki
Gary K
GK
D001427:Bacterial Toxins / Q000032:analysis* / Q000633:toxicity; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D017097:Electric Impedance; D004563:Electrochemistry; D004768:Enterotoxins / Q000032:analysis / Q000633:toxicity; D004867:Equipment Design; D006046:Gold / Q000737:chemistry; D053770:Nanowires / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012825:Silicon / Q000737:chemistry*; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors; D014173:Transistors, Electronic
D001427:Bacterial Toxins; D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal; D006046:Gold; D012825:Silicon