title: Nitrogen fixation island and rhizosphere competence traits in the genome of root-associated Pseudomonas stutzeri A1501.
abstract: The capacity to fix nitrogen is widely distributed in phyla of Bacteria and Archaea but has long been considered to be absent from the Pseudomonas genus. We report here the complete genome sequencing of nitrogen-fixing root-associated Pseudomonas stutzeri A1501. The genome consists of a single circular chromosome with 4,567,418 bp. Comparative genomics revealed that, among 4,146 protein-encoding genes, 1,977 have orthologs in each of the five other Pseudomonas representative species sequenced to date. The genome contains genes involved in broad utilization of carbon sources, nitrogen fixation, denitrification, degradation of aromatic compounds, biosynthesis of polyhydroxybutyrate, multiple pathways of protection against environmental stress, and other functions that presumably give A1501 an advantage in root colonization. Genetic information on synthesis, maturation, and functioning of nitrogenase is clustered in a 49-kb island, suggesting that this property was acquired by lateral gene transfer. New genes required for the nitrogen fixation process have been identified within the nif island. The genome sequence offers the genetic basis for further study of the evolution of the nitrogen fixation property and identification of rhizosphere competence traits required in the interaction with host plants; moreover, it opens up new perspectives for wider application of root-associated diazotrophs in sustainable agriculture.
authors.affiliation: Biotechnology Research Institute, National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Yongliang
authors.initials: Y
authors.lastname: Yan
authors.forename: Jian
authors.initials: J
authors.lastname: Yang
authors.forename: Yuetan
authors.initials: Y
authors.lastname: Dou
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.forename: Shuzhen
authors.initials: S
authors.lastname: Ping
authors.forename: Junping
authors.initials: J
authors.lastname: Peng
authors.forename: Wei
authors.initials: W
authors.lastname: Lu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Ziying
authors.initials: Z
authors.lastname: Yao
authors.forename: Hongquan
authors.initials: H
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Sheng
authors.initials: S
authors.lastname: He
authors.forename: Lizhao
authors.initials: L
authors.lastname: Geng
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Zhang
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.forename: Haiying
authors.initials: H
authors.lastname: Yu
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Zhan
authors.forename: Danhua
authors.initials: D
authors.lastname: Li
authors.forename: Zhanglin
authors.initials: Z
authors.lastname: Lin
authors.forename: Yiping
authors.initials: Y
authors.lastname: Wang
authors.forename: Claudine
authors.initials: C
authors.lastname: Elmerich
authors.forename: Min
authors.initials: M
authors.lastname: Lin
authors.forename: Qi
authors.initials: Q
authors.lastname: Jin
mesh_terms: D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000235:genetics; D020869:Gene Expression Profiling; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D005810:Multigene Family; D009586:Nitrogen Fixation / Q000235:genetics*; D009591:Nitrogenase / Q000235:genetics / Q000378:metabolism; D018517:Plant Roots / Q000382:microbiology*; D044223:Pseudomonas stutzeri / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA
chemical_list: D009591:Nitrogenase