Potential application of alkaline pectinase from Bacillus subtilis SS in pulp and paper industry.
Pectinase production from Bacillus subtilis SS was optimized under solid-state fermentation (5,943 U/g of dry bacterial bran). The pectinase produced was stable in neutral to alkaline pH range at 70 degrees C; therefore, the suitability of this pectinase in pulp and paper industry was investigated. The enzyme pretreatment process was optimized, and a pectinase dose of 5 IU/g of oven-dried pulp (10% consistency) at pH 9.5 temperature 70 degrees C after 150 min of treatment gave the best pretreatment to the pulp. An increase of 4.3% in brightness along with an increase of 14.8 and 65.3% in whiteness and fluorescence, respectively, whereas a 15% decrease in the yellowness of the pretreated pulp were observed. There was a 5.85% reduction in kappa number and 6.1% reduction in permanganate number along with a reduction in the chemical oxygen demand value. Significant characteristics showed by pectinase open new possibilities of application of this cellulase-free enzyme in the pulp and paper industry by reducing the negative environmental impact of chemicals apart from improving the properties of paper.
Ahlawat
Sonia
S
Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India.
Mandhan
R P
RP
Dhiman
Saurabh Sudha
SS
Kumar
Rakesh
R
Sharma
Jitender
J
D001412:Bacillus subtilis / Q000201:enzymology*; D010209:Paper; D011096:Polygalacturonase / Q000378:metabolism*; D013783:Textile Industry
D011096:Polygalacturonase