[Mutation of MTCYB and MTATP6 is associated with asthenospermia].
OBJECTIVE
To explore the correlation of the mutation of MTCYB and MTATP6 genes in sperm mitochondria with asthenospermia.


METHODS
We extracted mtDNA from 80 semen samples of asthenospermia and 20 of normal sperm motility, amplified the MTCYB and MTATP6 genes by PCR, and analyzed their mutation by sequencing and BLAST matching.


RESULTS
The deletion of both MTCYB and MTATP6 were detected in 20 of the 80 asthenospermia samples, MTCYB deletion in 16 and MTATP6 deletion in 4, accounting for 20% and 5% respectively. Sequencing and BLAST matching revealed G8887A mutation in the MTATP6 gene in the asthenospermia samples, with a mutation rate of 20%, while no regular mutation was noted in MTCYB. Neither significant deletion nor mutation was observed in any of the 20 samples of normal sperm motility.


CONCLUSIONS
Both the deletion and mutation of MTCYB and MTATP6 genes in sperm mitochondria might affect sperm motility in adults.
Feng
Chun-Qiong
CQ
Institute of Genetic engineering, Southern Medical University, Guangzhou, Guangdong 510515, China.
Song
Yan-Bin
YB
Zou
Ya-Guang
YG
Mao
Xiang-Ming
XM
D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics / Q000473:pathology*; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D006801:Humans; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D012689:Sequence Homology, Nucleic Acid; D013076:Sperm Count; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology
D004272:DNA, Mitochondrial; C501005:MT-ATP6 protein, human; D024101:Mitochondrial Proteins; D045303:Cytochromes b; D025261:Mitochondrial Proton-Translocating ATPases