Liquid crystalline phase of G-tetrad DNA for NMR study of detergent-solubilized proteins.
The liquid crystalline phase consisting of the potassium salt of the dinucleotide d(GpG) is compatible with detergents commonly used for solubilizing membrane proteins, including dodecylphosphocholine, the lysolipid 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine, and small bicelles consisting of dihexanoyl phosphatidylcholine and dimyristoyl phosphatidylcholine. The chiral nematic liquid crystalline phase of d(GpG) consists of long columns of stacked G-tetrad structures and carry a net negative charge. For water-soluble systems, the protein alignment induced by d(GpG) is very similar to that observed for liquid crystalline Pf1 bacteriophage, but of opposite sign. Alignment of the detergent-solubilized fusion domain of hemagglutinin is demonstrated to be homogeneous and stable, resulting in high quality NMR spectra suitable for the measurement of residual dipolar couplings.
Lorieau
Justin
J
Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892-0520, USA.
Yao
Lishan
L
Bax
Ad
A
D025561:Bacteriophage Pf1 / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry*; D015226:Dinucleoside Phosphates / Q000737:chemistry; D054856:G-Quadruplexes; D006388:Hemagglutinins / Q000737:chemistry; D050866:Liquid Crystals / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010713:Phosphatidylcholines / Q000737:chemistry; D011487:Protein Conformation; D012995:Solubility
D003902:Detergents; D015226:Dinucleoside Phosphates; D006388:Hemagglutinins; D008565:Membrane Proteins; D010713:Phosphatidylcholines; C018309:guanylyl-(3'-5')-guanosine; D004247:DNA