Expression of zinc transporter ZnT7 in mouse superior cervical ganglion.
The superior cervical ganglion (SCG) neurons contain a considerable amount of zinc ions, but little is known about the zinc homeostasis in the SCG. It is known that zinc transporter 7 (ZnT7, Slc30a7), a member of the Slc30 ZnT family, is involved in mobilizing zinc ions from the cytoplasm into the Golgi apparatus. In the present study, we examined the expression and localization of ZnT7 and labile zinc ions in the mouse SCG using immunohistochemistry, Western blot and in vivo zinc selenium autometallography (AMG). Our immunohistochemical analysis revealed that the ZnT7 immunoreactivity in the SCG neurons was predominately present in the perinuclear region of the neurons, suggesting an affiliation to the Golgi apparatus. The Western blot results verified that ZnT7 protein was expressed in the mouse SCGs. The AMG reaction product was shown to have a similar distribution as ZnT7 immunoreactivity. These observations support the notion that ZnT7 may participate in zinc transport, storage, and incorporation of zinc into zinc-binding proteins in the Golgi apparatus of mouse SCG neurons.
Gao
Hui-Ling
HL
Department of Histology and Embryology, China Medical University, Shenyang 110001, PR China.
Xu
He
H
Wang
Xin
X
Dahlstrom
Annica
A
Huang
Liping
L
Wang
Zhan-You
ZY
D000818:Animals; D001345:Autoradiography; D027682:Cation Transport Proteins / Q000378:metabolism*; D002451:Cell Compartmentation / Q000502:physiology; D006056:Golgi Apparatus / Q000378:metabolism / Q000648:ultrastructure; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D009448:Neurochemistry; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D017783:Superior Cervical Ganglion / Q000166:cytology* / Q000378:metabolism*; D015032:Zinc / Q000378:metabolism*
D027682:Cation Transport Proteins; C472293:ZnT7 protein, mouse; D015032:Zinc