title: [Changes of sperm chromatin in oligo-astheno-teratozoospermia syndrome patients after treated by integrated Chinese and Western medicine].
abstract: OBJECTIVE
  To observe the changes of sperm chromatin in patients with oligo-astheno-teratozoospermia (OAT) syndrome after treated by integrated Chinese and Western medicine.
  
  
  METHODS
  Sixty patients with OAT syndrome were treated by integrated Chinese and Western medicine for 3 months. Their sperm samples were collected before and after the treatment, subjected to acridine orange staining and analyzed by fluorescent microscopy, flow cytometry and sperm routine detection.
  
  
  RESULTS
  Significant differences were shown in the master-group sperm signals (P < 0.01) and at and COMPalphat (P < 0.05) by flow cytometry, as well as in the green and the red groups (P < 0.05) by fluorescent microscopy before and after the treatment. Changes in sperm concentration, motility, vitality and deformity were noted after the treatment, with statistic difference between pre- and post-treatment (P < 0.05) except in forward sperm concentration.
  
  
  CONCLUSIONS
  Treatment by integrated Chinese and Western medicine can improve sperm chromatin in patients with OAT syndrome. Flow cytometry, along with fluorescent microscopy and sperm routine detection, plays an important role in the evaluation of male infertility therapy.
authors.affiliation: Department of Urology, Affiliated Hospital of Hebei University of Engineering, Handan, Hubei 056029, China. dujiangping2004@126.com
authors.forename: Jiang-Ping
authors.initials: JP
authors.lastname: Du
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: Yang
authors.forename: Jin-E
authors.initials: JE
authors.lastname: Men
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Zhang
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Zheng
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
mesh_terms: D000328:Adult; D002843:Chromatin / Q000378:metabolism*; D003131:Combined Modality Therapy; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008856:Microscopy, Fluorescence; D009845:Oligospermia / Q000628:therapy*; D013076:Sperm Count; D013081:Sperm Motility; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology
chemical_list: D002843:Chromatin