Validation and standardization of virus neutralizing test using indirect immunoperoxidase technique for the quantification of antibodies to rabies virus.
A virus neutralizing test using an indirect immunoperoxidase technique (VNT-IIP) for rabies has been developed for the titration of dog and cat serum samples in Japan. The VNT-IIP has the advantage that results obtained can be viewed by the naked eye. The purpose of this study was to validate the VNT-IIP and compare it with one of the international standard methods, the fluorescent antibody virus neutralization test (FAVNT). The VNT-IIP showed satisfactory repeatability, high analytical specificity and good accuracy. Regarding the comparison between the VNT-IIP and the FAVNT, the VNT-IIP showed good agreement (91.9%), high sensitivity (92.8%) as well as specificity (87.0%) and good correlation (r = 0.92). As described above, the validation of the VNT-IIP was satisfactory and the performances of the test proved to be equivalent to those of an international standard method.
Ogawa
T
T
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo, Japan. ogawa@nval.go.jp
Gamoh
K
K
Aoki
H
H
Kobayashi
R
R
Etoh
M
M
Senda
M
M
Hirayama
N
N
Nishimura
M
M
Shiraishi
R
R
Servat
A
A
Cliquet
F
F
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002371:Cat Diseases / Q000175:diagnosis; D002415:Cats; D004283:Dog Diseases / Q000175:diagnosis; D004285:Dogs; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods / Q000662:veterinary*; D009500:Neutralization Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D011818:Rabies / Q000175:diagnosis / Q000662:veterinary; D011819:Rabies Vaccines / Q000276:immunology*; D011820:Rabies virus / Q000276:immunology* / Q000302:isolation & purification; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D011819:Rabies Vaccines