title: Esco2 is a novel corepressor that associates with various chromatin modifying enzymes.
abstract: Accurate chromosome segregation during cell division requires that sister chromatids are kept together by cohesin complex until anaphase, when the chromatids separate and distribute to the two daughter cells. Esco2 is an acetyltransferase that is required for the establishment of sister chromatid cohesion during S phase. Here, we report that Esco2 interacts with several component proteins of the CoREST complex, including a transcription corepressor CoREST, histone demethlyase LSD1, HDAC1, HDAC2, BRAF35, and PHF21A. Esco2 also interacts with various histone methyltransferases Suv39h1, SETDB1 and G9a. Esco2 complex purified from HeLa nuclear extract possesses histone H3 K9 methylation activity and functions as a transcription repressor. Esco2 fused to Gal4 DNA binding domain represses transcription by increasing methylation of histone H3 K9 in the promoter region. These results suggest a novel function of Esco2 in transcription repression through modulation of the chromatin structure.
authors.affiliation: Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, 300 Chunchun-dong, Jangan-gu, Suwon, Kyonggi-do 440-746, Republic of Korea.
authors.forename: Beom-Jun
authors.initials: BJ
authors.lastname: Kim
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Kang
authors.forename: Sung Yun
authors.initials: SY
authors.lastname: Jung
authors.forename: Hyun-Kyung
authors.initials: HK
authors.lastname: Choi
authors.forename: Jong-Hun
authors.initials: JH
authors.lastname: Seo
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Chae
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Cho
authors.forename: Hong-Duk
authors.initials: HD
authors.lastname: Youn
authors.forename: Jun
authors.initials: J
authors.lastname: Qin
authors.forename: Seong-Tae
authors.initials: ST
authors.lastname: Kim
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D002843:Chromatin / Q000201:enzymology*; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D000076983:Histone Methyltransferases; D011495:Histone-Lysine N-Methyltransferase / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism; D006801:Humans; D008780:Methyltransferases / Q000378:metabolism; D011496:Protein Methyltransferases / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D006657:Histones; D012097:Repressor Proteins; C407843:SUV39H1 protein, human; D000076983:Histone Methyltransferases; D008780:Methyltransferases; D011496:Protein Methyltransferases; D011495:Histone-Lysine N-Methyltransferase; C454062:SETDB1 protein, human; D000123:Acetyltransferases; C500785:ESCO2 protein, human