title: Effects of tanshinone IIA on the hepatotoxicity and gene expression involved in alcoholic liver disease.
abstract: Tanshinone IIA is one of the most abundant constituents of the root of Salvia miltiorrhiza BUNGE which exerts antioxidant and anti-inflammatory actions in many experimental disease models. In the present study, we demonstrated that the standardized fraction of S. miltiorrhiza (Sm-SF) was able to protect RAW 264.7 cells from ethanol-and lipopolysaccharide (LPS)-induced production of superoxide radical, activation of NADPH oxidase and subsequently death of the cells. Among four main components of Sm-SF, tanshinone IIA was the most potent in protecting cells from LPS-and ethanol-induced cytotoxicity. LPS or ethanol induced the expression of CD14, iNOS, and SCD1 and decreased RXR-alpha, which was completely reversed by tanshinone IIA. In H4IIEC3 cells, 10 microM tanshinone IIA effectively blocked ethanol-induced fat accumulation as evidenced by Nile Red binding assay. These results indicate that tanshinone IIA may have potential to inhibit alcoholic liver disease by reducing LPS-and ethanol-induced Kupffer cell sensitization, inhibiting synthesis of reactive oxygen/nitrogen species, inhibiting fatty acid synthesis and stimulating fatty acid oxidation.
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Hu-Quan
authors.initials: HQ
authors.lastname: Yin
authors.forename: Youn-Su
authors.initials: YS
authors.lastname: Kim
authors.forename: You-Jin
authors.initials: YJ
authors.lastname: Choi
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Sohn
authors.forename: Shi-Yong
authors.initials: SY
authors.lastname: Ryu
authors.forename: Byung-Hoon
authors.initials: BH
authors.lastname: Lee
mesh_terms: D045784:Abietanes; D000818:Animals; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D019610:Cytoprotection; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D000431:Ethanol / Q000494:pharmacology*; D020869:Gene Expression Profiling; D022781:Hepatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D050356:Lipid Metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008108:Liver Diseases, Alcoholic / Q000378:metabolism; D051379:Mice; D010616:Phenanthrenes / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D027561:Salvia miltiorrhiza
chemical_list: D045784:Abietanes; D004365:Drugs, Chinese Herbal; D008070:Lipopolysaccharides; D010616:Phenanthrenes; D010936:Plant Extracts; D017382:Reactive Oxygen Species; C021751:tanshinone; D000431:Ethanol