Copper-mediated DNA photocleavage by a tetrapyridoacridine (tpac) ligand.
We have focused our interest on the tetrapyridoacridine ligand tetrapyrido[3,2-a:2',3'-c:3'',2''-h: 2''',3'''-j]acridine (tpac), as a model system for the preparation of novel copper-based artificial nucleases. The complex of copper(II)-tpac cleaves supercoiled pUC18 plasmid DNA in an oxidative manner by photoactivation with visible light, exhibiting maximum cleaving efficiency at 1:2 metal-ligand stoichiometric ratio. We propose an interaction of the copper-tpac complex with DNA through both major and minor grooves and a photocleavage mechanism via the formation of hydroxyl radicals and singlet oxygen or singlet oxygen-like species.
Marta González-Alvarez
Departamento de Química Inorgánica, Universidad de Valencia, 46100-Burjassot, Valencia, Spain.
Arias
María-Selma
MS
Fernández
María-José
MJ
Gude
Lourdes
L
Lorente
Antonio
A
Alzuet
Gloria
G
Borrás
Joaquín
J
D000166:Acridines / Q000737:chemistry*; D003300:Copper / Q000737:chemistry* / Q000378:metabolism; D004247:DNA / Q000378:metabolism* / Q000528:radiation effects*; D003851:Deoxyribonucleases / Q000378:metabolism*; D008024:Ligands; D008027:Light; D008958:Models, Molecular; D015394:Molecular Structure; D010618:Phenanthrolines / Q000737:chemistry*; D010777:Photochemistry; D026082:Singlet Oxygen / Q000737:chemistry
D000166:Acridines; D008024:Ligands; D010618:Phenanthrolines; C529854:tetrapyridoacridine; D026082:Singlet Oxygen; D003300:Copper; D004247:DNA; D003851:Deoxyribonucleases