title: Investigation of Frizzled-5 during embryonic neural development in mouse.
abstract: Recent studies revealed that the Wnt receptor Frizzled-5 (Fzd5) is required for eye and retina development in zebrafish and Xenopus, however, its role during mammalian eye development is unknown. In the mouse embryo, Fzd5 is prominently expressed in the pituitary, distal optic vesicle, and optic stalk, then later in the progenitor zone of the developing retina. To elucidate the role of Fzd5 during eye development, we analyzed embryos with a germline disruption of the Fzd5 gene at E10.25, just before embryos die due to defects in yolk sac angiogenesis. We observed severe defects in optic cup morphogenesis and lens development. However, in embryos with conditional inactivation of Fzd5 using Six3-Cre, we observed no obvious early eye defects. Analysis of Axin2 mRNA expression and TCF/LEF-responsive reporter activation demonstrate that Fzd5 does not regulate the Wnt/beta-catenin pathway in the eye. Thus, the function of Fzd5 during eye development appears to be species-dependent.
authors.affiliation: Department of Neurobiology and Anatomy, John A. Moran Eye Center, University of Utah, Salt Lake City, Utah 84132, USA.
authors.forename: Carole J
authors.initials: CJ
authors.lastname: Burns
authors.forename: Jianmin
authors.initials: J
authors.lastname: Zhang
authors.forename: Erinn C
authors.initials: EC
authors.lastname: Brown
authors.forename: Alyssa M
authors.initials: AM
authors.lastname: Van Bibber
authors.forename: Johan
authors.initials: J
authors.lastname: Van Es
authors.forename: Hans
authors.initials: H
authors.lastname: Clevers
authors.forename: Tomo-o
authors.initials: TO
authors.lastname: Ishikawa
authors.forename: M Mark
authors.initials: MM
authors.lastname: Taketo
authors.forename: Monica L
authors.initials: ML
authors.lastname: Vetter
authors.forename: Sabine
authors.initials: S
authors.lastname: Fuhrmann
mesh_terms: D000818:Animals; D016923:Cell Death; D049109:Cell Proliferation; D005123:Eye / Q000196:embryology*; D051157:Frizzled Receptors / Q000096:biosynthesis* / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D007908:Lens, Crystalline / Q000196:embryology; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation; D009474:Neurons / Q000378:metabolism*; D009900:Optic Nerve / Q000196:embryology; D010902:Pituitary Gland / Q000378:metabolism; D043562:Receptors, G-Protein-Coupled / Q000096:biosynthesis* / Q000502:physiology*; D012160:Retina / Q000196:embryology; D013997:Time Factors
chemical_list: D051157:Frizzled Receptors; C403211:Fzd5 protein, mouse; D043562:Receptors, G-Protein-Coupled