Determination of Schistosoma japonicum circulating antigens in dilution serum by piezoelectric immunosensor and S/N enhancement.
A piezoelectric immunosensor assay was developed with immobilizing immunoglobulin G (IgG) as a probe to detect Schistosoma japonicum circulating antigens (SjCAg). Analytical strategy utilizes the polyclonal antibodies with broad-spectrum recognition to a complex target with high specificity. The immobilized antibodies were purified from immunized rabbit's sera (im-S) and infected rabbit's sera (inf-S) by S. japonicum. The detection capacities of antibodies were compared between the sera of different phenotypes and purified fractions. The sample dilution ratios were also evaluated and optimized. Additionally, the sera with a variety of infection degrees for validation could be discriminated quantitatively. The linear dose-response relationship indicates that the systematic sensitivity of this method is below 150 Hz and the lowest limit of detectable range is above 500 cercariae of S. japonicum infection for 2 weeks. The novel immunosensor technique is well potential to determine the SjCAg in serum samples for clinical diagnosis of parasitosis in early stage.
Cheng
Chien-Wei
CW
Department of Biotechnology, Ming-Chuan University, Gui-Shan, Taiwan.
Chen
Ching-Kuo
CK
Chen
Yi-Shen
YS
Chen
Liang-Yu
LY
D000818:Animals; D000947:Antigens, Helminth / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D011791:Quartz / Q000737:chemistry; D012549:Schistosoma japonicum / Q000276:immunology*
D000947:Antigens, Helminth; D011791:Quartz