title: [Mutation of MTCYB and MTATP6 is associated with asthenospermia].
abstract: OBJECTIVE
  To explore the correlation of the mutation of MTCYB and MTATP6 genes in sperm mitochondria with asthenospermia.
  
  
  METHODS
  We extracted mtDNA from 80 semen samples of asthenospermia and 20 of normal sperm motility, amplified the MTCYB and MTATP6 genes by PCR, and analyzed their mutation by sequencing and BLAST matching.
  
  
  RESULTS
  The deletion of both MTCYB and MTATP6 were detected in 20 of the 80 asthenospermia samples, MTCYB deletion in 16 and MTATP6 deletion in 4, accounting for 20% and 5% respectively. Sequencing and BLAST matching revealed G8887A mutation in the MTATP6 gene in the asthenospermia samples, with a mutation rate of 20%, while no regular mutation was noted in MTCYB. Neither significant deletion nor mutation was observed in any of the 20 samples of normal sperm motility.
  
  
  CONCLUSIONS
  Both the deletion and mutation of MTCYB and MTATP6 genes in sperm mitochondria might affect sperm motility in adults.
authors.affiliation: Institute of Genetic engineering, Southern Medical University, Guangzhou, Guangdong 510515, China.
authors.forename: Chun-Qiong
authors.initials: CQ
authors.lastname: Feng
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Song
authors.forename: Ya-Guang
authors.initials: YG
authors.lastname: Zou
authors.forename: Xiang-Ming
authors.initials: XM
authors.lastname: Mao
mesh_terms: D000328:Adult; D053627:Asthenozoospermia / Q000235:genetics / Q000473:pathology*; D001483:Base Sequence; D045303:Cytochromes b / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000235:genetics; D006801:Humans; D008297:Male; D024101:Mitochondrial Proteins / Q000235:genetics; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D012689:Sequence Homology, Nucleic Acid; D013076:Sperm Count; D013094:Spermatozoa / Q000378:metabolism / Q000473:pathology
chemical_list: D004272:DNA, Mitochondrial; C501005:MT-ATP6 protein, human; D024101:Mitochondrial Proteins; D045303:Cytochromes b; D025261:Mitochondrial Proton-Translocating ATPases