[Study on human papillomavirus infection and loss of heterozygosity of microsatellite in esophageal cancer].
OBJECTIVE
To investigate the relationship between the loss of heterozygosity(LOH) at 14 microsatellites in esophageal cancer and human papillomavirus (HPV) infection.


METHODS
HPV-16,18 DNA was examined in 112 tumor specimens using fluorescence quantitative PCR. 112 tumor specimens and their matched blood DNAs were analyzed for LOH at 14 microsatellites by PCR and fluorescence-based DNA sequencing technology. The frequencies of LOH at 14 microsatellites were compared between HPV positive and negative specimens.


RESULTS
High frequency of LOH was observed among chromosome arms 3p, 9p, 13q, 17p and 17q. The frequency of LOH was significantly higher at loci D13S260 and D6S497 in HPV positive specimens, comparing with HPV negative ones.


CONCLUSIONS
The findings regarding loci with allele loss indicated that widespread chromosome instability might have existed in esophageal cancer. HPV positive specimens with higher frequency of LOH than negative ones at locus D13S260 and D6S497 suggesting that the target of HPV in esophageal cancer might serve as candidate genes at these two loci. In addition,this result also indicated that HPV might be a high-risk factor for esophageal cancer in Sichuan area with a high incidence of this cancer.
Liu
Ming
M
Affiliated South-West Hospital, Third Military Medical University, Chongqing 400038,China.
Zeng
Hui-Chang
HC
Zhang
Xiao-Li
XL
Zhu
Jing
J
Huang
Jun-Fu
JF
Zhang
Xue
X
Xia
Mei
M
D000328:Adult; D000368:Aged; D043171:Chromosomal Instability / Q000235:genetics; D004938:Esophageal Neoplasms / Q000209:etiology / Q000235:genetics* / Q000821:virology*; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D019656:Loss of Heterozygosity / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D008875:Middle Aged; D030361:Papillomavirus Infections / Q000503:physiopathology*; D016133:Polymerase Chain Reaction