Variations in arsenic methylation capacity and oxidative DNA lesions over a 2-year period in a high arsenic-exposed population.
OBJECTIVE
To assess long-term variations in arsenic methylation and oxidative DNA lesions of chronic high arsenic-exposed populations.


METHODS
A follow-up study was conducted in 64 chronic high arsenic-exposed subjects from 2004 to 2006. Urinary arsenic species and 8-hydroxydeoxyguanine were measured.


RESULTS
Percentages of urinary inorganic arsenic, monomethylarsonate and urinary 8-hydroxydeoxyguanine (8-OHdG) level were significantly higher, but the percentage of dimethylarsinate, the primary methylation index (PMI) and secondary methylation index (SMI) was lower in the ninth year of arsenic exposure compared with the seventh year. Substantial differences in relative arsenic methylation capacity were observed between the seventh and ninth year. Percentages of arsenic species, PMI and SMI were significantly correlated between siblings, and between parents and children.


CONCLUSIONS
Arsenic methylation may decrease, but oxidative DNA lesions may increase with the increase of cumulative arsenic exposure level. Both genetic and environmental factors may contribute to variability in arsenic methylation.
Xu
Yuan-yuan
YY
Department of Occupational and Environmental Health, College of Public Health, China Medical University, Heping District, Shenyang, Liaoning, People's Republic of China.
Wang
Yi
Y
Li
Xin
X
He
Miao
M
Xue
Peng
P
Fu
Jing-qi
JQ
Wang
Hui-hui
HH
Sun
Gui-fan
GF
D000080242:8-Hydroxy-2'-Deoxyguanosine / Q000031:analogs & derivatives; D000293:Adolescent; D000328:Adult; D020261:Arsenic Poisoning / Q000235:genetics / Q000378:metabolism*; D001152:Arsenicals / Q000009:adverse effects* / Q000378:metabolism; D002648:Child; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004781:Environmental Exposure; D004785:Environmental Pollutants / Q000378:metabolism / Q000633:toxicity*; D005260:Female; D006147:Guanine / Q000031:analogs & derivatives / Q000652:urine; D006801:Humans; D008297:Male; D008745:Methylation; D008875:Middle Aged; D018384:Oxidative Stress; D013548:Sweden; D055815:Young Adult
D001152:Arsenicals; D004785:Environmental Pollutants; D006147:Guanine; C078307:8-oxo-7,8-dihydrodeoxyguanine; D000080242:8-Hydroxy-2'-Deoxyguanosine; D004247:DNA