GDNF blended chitosan nerve guides: an in vivo study.
Chitosan nerve guides are currently being utilized to repair damaged or injured peripheral nerves. To enhance the nerve regeneration process, instead of using the material alone, researchers are focusing on blending different proteins or molecules with chitosan to facilitate nerve repair and regeneration. In our study, we have blended chitosan with glial cell-line derived neurotrophic factor and laminin within our nerve guides (GLC). The rat sciatic nerve injury model was used to test these nerve guides and histologically evaluated at 6, 9, and 12 weeks. Histologically at 6 weeks, the axon area and myelination are significantly higher in the GLC group compared with the controls. However, at 9 and 12 weeks control groups matched the GLC values. Thus the histological results indicate that GLC nerve guides can enhance the nerve regeneration process during the initial stages of nerve repair.
Patel
Minal
M
Biomedical Engineering, Wayne State University, Detroit, Michigan 48201, USA.
Mao
Li
L
Wu
Bin
B
VandeVord
Pamela
P
D000818:Animals; D001369:Axons / Q000502:physiology* / Q000648:ultrastructure; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D048271:Chitosan / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000737:chemistry / Q000378:metabolism*; D048091:Guided Tissue Regeneration / Q000379:methods*; D020007:Implants, Experimental; D007797:Laminin / Q000737:chemistry / Q000378:metabolism; D008422:Materials Testing; D009186:Myelin Sheath / Q000378:metabolism / Q000648:ultrastructure; D009416:Nerve Regeneration / Q000502:physiology*; D051381:Rats; D011917:Rats, Inbred Lew; D012584:Sciatic Nerve / Q000166:cytology / Q000473:pathology / Q000502:physiology; D054457:Tissue Scaffolds / Q000737:chemistry*
D001672:Biocompatible Materials; D051100:Glial Cell Line-Derived Neurotrophic Factor; D007797:Laminin; D048271:Chitosan