The reduction of arterial tension produced by stevioside is dependent on nitric oxide synthase activity when the endothelium is intact.
In endothelium-intact rat aortic ring preparations pre-contracted with norepinephrine or KCl, NG-nitro L-arginine (L-NOARG, 0.1 mM) and 1H-[1,2,4] oxidiazolo [4,3-a] quinoxalin-1-one (ODQ, 10 microM) antagonized the reduction of the vascular tone induced by stevioside, but this antagonism did not occur when the experiment was performed with endothelium-denuded aortic rings. The data indicates that the vasodilatation produced by stevioside is dependent on nitric oxide synthase and guanylate cyclase activities when the endothelium is not damaged.
Bornia
Elaine Campana Sanches
EC
Department of Pharmacy and Pharmacology, State University of Maringa, Maringa-PR, Brazil.
do Amaral
Valéria
V
Bazotte
Roberto Barbosa
RB
Alves-Do-Prado
Wilson
W
D000818:Animals; D001013:Aorta, Thoracic; D045786:Diterpenes, Kaurane / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000201:enzymology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005960:Glucosides / Q000494:pharmacology*; D006162:Guanylate Cyclase / Q000037:antagonists & inhibitors / Q000378:metabolism; D066298:In Vitro Techniques; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors / Q000378:metabolism*; D019335:Nitroarginine / Q000494:pharmacology; D009638:Norepinephrine / Q000494:pharmacology; D010069:Oxadiazoles / Q000494:pharmacology; D011189:Potassium Chloride / Q000494:pharmacology; D011810:Quinoxalines / Q000494:pharmacology; D051381:Rats; D013549:Sweetening Agents / Q000494:pharmacology*; D014661:Vasoconstriction / Q000187:drug effects; D014662:Vasoconstrictor Agents / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects*
C095284:1H-(1,2,4)oxadiazolo(4,3-a)quinoxalin-1-one; D045786:Diterpenes, Kaurane; D004791:Enzyme Inhibitors; D005960:Glucosides; D010069:Oxadiazoles; D011810:Quinoxalines; D013549:Sweetening Agents; D014662:Vasoconstrictor Agents; C012043:stevioside; D019335:Nitroarginine; D011189:Potassium Chloride; D019001:Nitric Oxide Synthase; D006162:Guanylate Cyclase; D009638:Norepinephrine