Filter paper degrading ability of a Trichoderma strain with multinucleate conidia.
The multinucleate conidia were produced from the green mature conidia of Trichoderma reesei Rut C-30 strain by colchicine treatment. The strain with higher Filter paper degrading ability was selected among those conidia using a double layer selection medium. The selected strain, JS-2 was able to collapse the filter paper within 15 min but the original strain took 25 min to collapse it completely. Moreover, the amount of reducing sugar in the L-type glass tube of the strain, JS-2 was greater than that of the original strain. The Avicel, CMC-Na, and Salicin hydrolyzing activity of the strain, JS-2, increased 2.1 times, 1.2 times, and 3.6 times higher than that of the original strain.
Toyama
Hideo
H
Minamikyushu University, Kirishima 5-1-2, 880-0032, Miyazaki, Japan. wonder@iris.dti.ne.jp
Yano
Makiko
M
Hotta
Takeshi
T
Toyama
Nobuo
N
D002480:Cellulase / Q000737:chemistry* / Q000378:metabolism*; D004789:Enzyme Activation; D005374:Filtration / Q000295:instrumentation* / Q000379:methods*; D010209:Paper; D013172:Spores, Fungal / Q000201:enzymology*; D014242:Trichoderma / Q000201:enzymology*
D002480:Cellulase