Evaluation of RGD- or EGF-immobilized chitosan scaffolds for chondrogenic activity.
Chitosan scaffolds were prepared by freeze-drying method and modified with Arg-Gly-Asp (RGD) sequence of fibronectin or epidermal growth factor (EGF) by covalent immobilization. The results obtained from FTIR-ATR, fluorescence visualization and quantitative measurements showed that biosignal molecules, RGD and EGF, were successfully immobilized on chitosan scaffolds. ATDC5 murine chondrogenic cells were seeded on both type of scaffolds, chitosan-RGD and chitosan-EGF, and cultured for 28 days in stationary conditions. According to the results of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT) test, considerable increase in cell proliferation was only detected on chitosan-EGF scaffolds. Biochemical analysis of the chondrocyte seeded scaffolds showed that glycosaminoglycan (GAG) and deoxyribonucleic acid (DNA) content of the scaffolds increases with time. In conclusion, EGF-modified chitosan scaffolds (containing 1.83 microg EGF/3 mg dry scaffold) have been proposed to promote chondrogenesis and to have potential for reticular cartilage regeneration.
Tiğli
R Seda
RS
Hacettepe University, Chemical Engineering Department, 06800 Beytepe, Ankara, Turkey.
Gümüşderelioğlu
Menemşe
M
D000704:Analysis of Variance; D048271:Chitosan / Q000737:chemistry*; D004815:Epidermal Growth Factor / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013778:Tetrazolium Salts; D013844:Thiazoles; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D009842:Oligopeptides; D013778:Tetrazolium Salts; D013844:Thiazoles; D004815:Epidermal Growth Factor; C047981:arginyl-glycyl-aspartic acid; D048271:Chitosan; C022616:thiazolyl blue