title: A modified mini-primer set for analyzing mitochondrial DNA control region sequences from highly degraded forensic samples.
abstract: To facilitate the analysis of mitochondrial DNA (mtDNA) control region sequences from highly degraded skeletal remains, a modified mini-primer set was designed to overcome the limitations of the Armed Forces DNA Identification Laboratory (AFDIL) mini-primer set. This modified mini-primer set is less affected by nucleotide variability and PCR amplification conditions than the AFDIL mini-primer set, and was able to amplify the mtDNA sequences of 55-year-old skeletal remains with high efficiency, indicating that it is a useful tool for analyzing mtDNA control region sequences from highly degraded forensic samples.
authors.affiliation: Department of Forensic Medicine, Yonsei University College of Medicine, Yonsei University, Seoul, Korea.
authors.forename: Hwan Young
authors.initials: HY
authors.lastname: Lee
authors.forename: Na Young
authors.initials: NY
authors.lastname: Kim
authors.forename: Myung Jin
authors.initials: MJ
authors.lastname: Park
authors.forename: Woo Ick
authors.initials: WI
authors.lastname: Yang
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Shin
mesh_terms: D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000737:chemistry; D018732:Forensic Anthropology / Q000379:methods*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013997:Time Factors
chemical_list: D017931:DNA Primers; D004272:DNA, Mitochondrial