title: Guadinomines, Type III secretion system inhibitors, produced by Streptomyces sp. K01-0509. I: taxonomy, fermentation, isolation and biological properties.
abstract: Enteropathogenic Escherichia coli (EPEC) expressing the Type III secretion system (TTSS) induced hemolysis of sheep blood cells. Using this assay, six structurally related compounds designated as guadinomines were isolated as inhibitors of TTSS-induced hemolysis by ion exchange column chromatography and HPLC from the culture broth of Streptomyces sp. K01-0509. Guadinomines A and B showed potent inhibition with IC50 values of 0.02 and 0.007 microg/ml, respectively, guadinomine D showed moderate activity (IC50: 8.5 microg/ml), while guadinomines C1 and C2 and guadinomic acid had no activity.
authors.affiliation: The Kitasato Institute, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Iwatsuki
authors.forename: Ryuji
authors.initials: R
authors.lastname: Uchida
authors.forename: Hitomi
authors.initials: H
authors.lastname: Yoshijima
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ui
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
authors.forename: Atsuko
authors.initials: A
authors.lastname: Matsumoto
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Akio
authors.initials: A
authors.lastname: Abe
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomoda
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
mesh_terms: D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000302:isolation & purification / Q000494:pharmacology; D004151:Dipeptides / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D005285:Fermentation; D048289:Imidazolidines / Q000097:blood* / Q000302:isolation & purification / Q000494:pharmacology; D013302:Streptomyces / Q000145:classification* / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D004151:Dipeptides; D048289:Imidazolidines; C530702:guadinomine A; C530703:guadinomine B; C530704:guadinomine C; C530705:guadinomine D; C530706:guadinomine E