title: Diversity surveys and evolutionary relationships of aoxB genes in aerobic arsenite-oxidizing bacteria.
abstract: A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.
authors.affiliation: BRGM, Ecotechnology Unit, Process and Environment Division, 3 Avenue Claude Guillemin, 45060 Orléans Cedex 02, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Quéméneur
authors.forename: Audrey
authors.initials: A
authors.lastname: Heinrich-Salmeron
authors.forename: Daniel
authors.initials: D
authors.lastname: Muller
authors.forename: Didier
authors.initials: D
authors.lastname: Lièvremont
authors.forename: Michel
authors.initials: M
authors.lastname: Jauzein
authors.forename: Philippe N
authors.initials: PN
authors.lastname: Bertin
authors.forename: Francis
authors.initials: F
authors.lastname: Garrido
authors.forename: Catherine
authors.initials: C
authors.lastname: Joulian
mesh_terms: D000595:Amino Acid Sequence; D018053:Arsenites / Q000378:metabolism*; D001419:Bacteria / Q000201:enzymology* / Q000235:genetics*; D001483:Base Sequence; D044822:Biodiversity; D052837:Chemoautotrophic Growth; D017124:Conserved Sequence; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification; D005798:Genes, Bacterial; D005819:Genetic Markers; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000201:enzymology / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D020539:Sequence Analysis, Protein; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000378:metabolism
chemical_list: D018053:Arsenites; D017931:DNA Primers; D004269:DNA, Bacterial; D005819:Genetic Markers; D012336:RNA, Ribosomal, 16S; D012989:Soil Pollutants; D010088:Oxidoreductases; C078179:arsenite oxidase; C015001:arsenite