title: Biological value of melanoma inhibitory activity serum concentration in patients with primary skin melanoma.
abstract: Melanoma inhibitory activity (MIA) protein was identified in significant quantities in primary and metastatic malignant melanomas, where it has an important role in promoting tumor development and progression. Our hypothesis was that MIA serum level will be elevated in patients with metastases or local spreading of the disease before any symptom of such progression is clinically apparent. We compared MIA serum levels in two groups of patients with primary melanoma; those with positive as opposed to those with negative sentinel lymph nodes. In addition, MIA serum levels were studied in two control groups; patients with dysplastic nevi and patients with basal cell carcinoma. A blood sample was obtained from each patient included in the study and MIA levels were assessed using standard enzyme-linked immunosorbent assay method. Patients with histologically positive sentinel lymph nodes, meaning that tumor cells were found in the lymph nodes, had much higher mean MIA values than any other patient group considered in this study. With mean value of 14.53 ng/ml, it was almost twice as high as mean MIA value in patients with histologically negative sentinel lymph nodes (7.32 ng/ml) and more than twice as high than any of the two control groups (P<0.001). However, neither the classification by Clarke nor the classification by Breslow could be used to distinguish patients with positive sentinel lymph nodes from those with negative sentinel lymph nodes. In our opinion, MIA serum level is the ideal test for screening the tumor spread to sentinel lymph nodes.
authors.affiliation: Department of Surgery, University Hospital Sestre Milosrdnice, Vinogradska, Croatia.
authors.forename: Borki
authors.initials: B
authors.lastname: Vucetić
authors.forename: Suncica Andreja
authors.initials: SA
authors.lastname: Rogan
authors.forename: Pero
authors.initials: P
authors.lastname: Hrabac
authors.forename: Narcis
authors.initials: N
authors.lastname: Hudorović
authors.forename: Hrvoje
authors.initials: H
authors.lastname: Cupić
authors.forename: Ljerka
authors.initials: L
authors.lastname: Lukinac
authors.forename: Mario
authors.initials: M
authors.lastname: Ledinsky
authors.forename: Aljosa
authors.initials: A
authors.lastname: Matejcić
authors.forename: Ivo
authors.initials: I
authors.lastname: Lovricević
authors.forename: Mirta
authors.initials: M
authors.lastname: Zekan
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis / Q000097:blood; D002280:Basal Cell Carcinoma / Q000097:blood / Q000473:pathology; D004416:Dysplastic Nevus Syndrome / Q000097:blood; D016326:Extracellular Matrix Proteins / Q000097:blood*; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008545:Melanoma / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000097:blood*; D011237:Predictive Value of Tests; D011379:Prognosis; D012016:Reference Values; D012680:Sensitivity and Specificity; D021701:Sentinel Lymph Node Biopsy; D012878:Skin Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; D016326:Extracellular Matrix Proteins; C090059:MIA protein, human; D009363:Neoplasm Proteins