Analysis of immune complexes by capillary electrophoresis.
A simple method for immune complexes (IC) analysis by CE is described. This method combines the ease of precipitation of the IC by polyethylene glycol with the separation power of CE. The advantage of this method is a better quantitation of the IC, since it corrects and eliminates the interferences from other serum proteins. It also reveals the composition (monoclonality) of the precipitate. Three types of IC have been detected in this method: monoclonal, polyclonal and mixed (mono-polyclonal) IC. Furthermore, the method is rapid and simple.
Shihabi
Zak K
ZK
Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, NC, USA. zshihabi@wfubmc.edu
D000510:Alpha-Globulins / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000032:analysis; D000936:Antigen-Antibody Complex / Q000032:analysis*; D001609:Beta-Globulins / Q000302:isolation & purification; D011232:Chemical Precipitation; D019075:Electrophoresis, Capillary; D006801:Humans; D007239:Infections / Q000276:immunology; D007674:Kidney Diseases / Q000276:immunology; D011092:Polyethylene Glycols; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis; D005719:gamma-Globulins / Q000302:isolation & purification
D000510:Alpha-Globulins; D000911:Antibodies, Monoclonal; D000936:Antigen-Antibody Complex; D001609:Beta-Globulins; D012709:Serum Albumin; D005719:gamma-Globulins; D011092:Polyethylene Glycols