[Transforming growth factor beta upregulates the expression of invasion and metastasis associated proteins in prostate cancer LNCaP cell lines in vitro].
OBJECTIVE
To determine the effect of the transforming growth factor beta (TGF-beta) on the expression of invasion and metastasis associated proteins in the prostate cancer LNCaP cell line in vitro.


METHODS
The prostate cancer cell line LNCaP was treated with TGF-beta in vitro. Western blotting was used to detect the expression of the "invasion and metastasis" associated proteins E-Cadherin, N-Cadherin and Vimentin.


RESULTS
The expression of N-Cadherin and Vimentin of the LNCaP cells treated with TGF-beta for 12 hours was significantly upregulated, but not that of E-Cadherin.


CONCLUSIONS
TGF-beta may induce epithelial-mesenchymal transition (EMT) of LNCaP cells which might be of importance in promoting prostate cancer cells invading to ambient tissues and metastasizing to distant organs.
Zhu
Guang-Feng
GF
Urology Institute of Xi'an Jiaotong University, Department of Urology, the First Affiliated Hospital, Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, China. zhuguangfeng@stu.xjtu.edu.cn
Cheng
He-Peng
HP
Wu
Kai-Jie
KJ
Zhang
Lin-Lin
LL
Zhu
Guo-Dong
GD
Zhang
Dong
D
He
Da-Lin
DL
D015153:Blotting, Western; D015820:Cadherins / Q000096:biosynthesis; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D016212:Transforming Growth Factor beta / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects; D014746:Vimentin / Q000096:biosynthesis
D015820:Cadherins; D016212:Transforming Growth Factor beta; D014746:Vimentin