title: [Protecting mice from fatal Bordetella bronchiseptica infection by immunization with recombinant pertactin antigens].
abstract: OBJECTIVE
  We evaluated the efficacy of the recombinant pertactin (PRN)-specific active or passive immunization against Bordetella bronchiseptica (Bb) in an aerosol challenge model established by using BALB/c mice.
  
  
  RESULTS
  Mice, immunized subcutaneously with two doses of purified glutathione S-transferase (GST)-PRN protein mixed with an equal volume of Freund's adjuvant, produced robust PRN-specific IgG antibody activity. All 20 mice vaccinated with GST-PRN protein survived aerosol challenge with three times the 50% lethal dose (LD50) of virulent Bb HH0809 compared with 3 of 20 GST protein-treated controls and 4 of 20 PBS-treated controls that survived. Furthermore, we observed complete protection against intraperitoneal challenge with ten times the LD50 of virulent HH0809 strain in mice that were injected intraperitoneally with 0.5 ml rabbit anti-GST-PRN serum. No survivors were observed in mice that received either rabbit anti-GST serum or PBS alone.
  
  
  CONCLUSIONS
  The recombinant PRN protein had strong immunogenicity against Bb infection.
authors.affiliation: State Key Laboratory of Agriculture Microbiology, College of Veterinary Medicine, Huazhong Agriculture University, Wuhan 430070, China. zhaozhanqin@126.com
authors.forename: Zhanqin
authors.initials: Z
authors.lastname: Zhao
authors.forename: Yun
authors.initials: Y
authors.lastname: Xue
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: Longchuan
authors.initials: L
authors.lastname: Duan
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.forename: Xibiao
authors.initials: X
authors.lastname: Tang
authors.forename: Ruiming
authors.initials: R
authors.lastname: Hu
authors.forename: Hua
authors.initials: H
authors.lastname: He
authors.forename: Zengqiang
authors.initials: Z
authors.lastname: Li
mesh_terms: D000336:Aerosols; D000818:Animals; D000906:Antibodies / Q000032:analysis / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001885:Bordetella Infections / Q000276:immunology* / Q000517:prevention & control*; D016950:Bordetella bronchiseptica / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization / Q000379:methods*; D007116:Immunization, Passive; D008168:Lung / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000276:immunology*; D014611:Vaccination; D010566:Virulence Factors, Bordetella / Q000276:immunology*
chemical_list: D000336:Aerosols; D000906:Antibodies; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D011994:Recombinant Proteins; D010566:Virulence Factors, Bordetella; C068929:pertactin