title: The effect of an interleukin receptor antagonist (IL-1ra) on colonocyte eicosanoid release.
abstract: We investigated whether an interleukin 1 receptor antagonist (IL-1ra) altered cellular release of prostanoids and leukotrienes in a transformed colonic cell line (CACO-2) in the presence of proinflammatory stimuli. Cellular inflammation was induced by treatment with lipopolysaccharide (LPS) or the cytokine, interleukin 1 beta (IL-1(beta)). In a separate set of experiments, cells were pretreated with IL-1ra prior to exposure to LPS or IL-1(beta). Prostaglandin E(2) and leukotriene B(4) (LTB(4)) levels were quantified by ELISA assays. Both LPS and IL-1(beta) exposure were noted to stimulate cellular PGE(2) release, a response which was significantly inhibited by IL-1ra treatment. Either stimulant when administered alone failed to stimulate release of LTB(4). When administered after IL-1ra pretreatment however, both stimuli caused a significant increase in LTB(4) release. These results suggest that a cytokine receptor antagonist can selectively influence eicosanoid production in this cell line. Furthermore, this study suggests that a IL-1ra may have a future clinical role in the treatment of inflammatory disorders of the colon which are intimately linked to enhanced eicosanoid synthesis.
authors.affiliation: Department of Surgery Saint Louis University School of Medicine Health Sciences Center 3635 Vista Avenue at Grand Boulevard St Louis Missouri 63110-0250 USA.
authors.forename: G S
authors.initials: GS
authors.lastname: Smith
authors.forename: C
authors.initials: C
authors.lastname: Rieckenberg
authors.forename: W E
authors.initials: WE
authors.lastname: Longo
authors.forename: D L
authors.initials: DL
authors.lastname: Kaminski
authors.forename: J E
authors.initials: JE
authors.lastname: Mazuski
authors.forename: Y
authors.initials: Y
authors.lastname: Deshpande
authors.forename: T A
authors.initials: TA
authors.lastname: Miller