title: [Polymorphisms of UGT1A gene and irinotecan toxicity in Chinese colorectal cancer patients].
abstract: OBJECTIVE
  To assess the polymorphism of UGT1A gene in Chinese, and to investigate the correlation between UGT1A polymorphism and irinotecan toxicity in colorectal cancer patients.
  
  
  METHODS
  70 patients with advanced colorectal cancer were treated with irinotecan and 5-fluorouracil. Polymorphism analysis was performed in all those patients and 100 healthy subjects. Genomic DNA was extracted from peripheral blood and genotyped using polymerase chain reaction and direct sequencing.
  
  
  RESULTS
  14 patients exhibiting grade 3 - 4 neutropenia (20.0%), 16 patients experienced grade 2 - 4 diarrhea (22.9%), including only 4 patients with grade 3 - 4 diarrhea (5.7%). Compared with TA6/7 and TA7/7, UGT1 A1 * 28 wild genotype TA6/6 was significantly associated with reduced toxicity (42.1% vs. 15.7%, P = 0.027). There was no significant difference in the distribution of UGT1A genotypes between colorectal cancer patients and healthy subjects.
  
  
  CONCLUSIONS
  Chinese patients exhibit less irinotecan-related diarrhea due to higher frequence of UGT1A A1 * 28 wild genotype TA6/6.
authors.affiliation: Cancer Center, 307 Hospital, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.forename: Jian-Ming
authors.initials: JM
authors.lastname: Xu
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.forename: Nong
authors.initials: N
authors.lastname: Xu
authors.forename: Jin-Wan
authors.initials: JW
authors.lastname: Wang
authors.forename: Shun-Chang
authors.initials: SC
authors.lastname: Jiao
authors.forename: Jing-Sheng
authors.initials: JS
authors.lastname: Zhang
authors.forename: San-Tai
authors.initials: ST
authors.lastname: Song
authors.forename: Jian
authors.initials: J
authors.lastname: Li
authors.forename: Han-Ying
authors.initials: HY
authors.lastname: Bao
authors.forename: Lin
authors.initials: L
authors.lastname: Yang
authors.forename: Fang
authors.initials: F
authors.lastname: Li
mesh_terms: D000328:Adult; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000009:adverse effects*; D000971:Antineoplastic Combined Chemotherapy Protocols / Q000009:adverse effects / Q000627:therapeutic use; D044466:Asian People / Q000235:genetics; D002166:Camptothecin / Q000008:administration & dosage / Q000009:adverse effects / Q000031:analogs & derivatives*; D016022:Case-Control Studies; D002681:China; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000235:genetics*; D003967:Diarrhea / Q000139:chemically induced*; D005472:Fluorouracil / Q000008:administration & dosage; D020022:Genetic Predisposition to Disease; D005838:Genotype; D014453:Glucuronosyltransferase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D000077146:Irinotecan; D009503:Neutropenia / Q000139:chemically induced; D011110:Polymorphism, Genetic; D055815:Young Adult
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000077146:Irinotecan; D014453:Glucuronosyltransferase; D005472:Fluorouracil; D002166:Camptothecin