[Research of gene chip detection and gene expression profile of nasal polyps].
OBJECTIVE
To investigate the gene expression profile by using gene chip technology and probe into the role of corresponding gene in the pathogenesis of nasal polyps by analysing the difference of the gene depression.


METHODS
The total RNAs were respectively extracted from 6 pairs of inferior turbinates and nasal polyps, and then were reversely transcribed to cDNAs with incorporation of fluorescent dUTP as the hybridization probes. The mixed probes were then hybridized with the BiostarH-40 s gene chips, it was scanned by laser scanner and the acquired image was analyzed by software.


RESULTS
1887 genes were differently expressed in gene profile of nasal polyps, among which 1099 were upregulated and 788 were down-regulated. Six genes were found in all gene chips, among which 4 genes were upregulated and 2 were down-regulated. The 6 genes encoded the protein of the transmembrane 4 superfamily, highly similar to GAMMA-interferon-inducible protein IP-30 precursor, highly similar to complement factor I precursor and insulin-like growth factor binding protein 3 (IGFBP3).


CONCLUSIONS
Detecting the differently expressed genes will provide clues and theoretical foundation for the pathogenesis of nasal polyps. The nasal polyps is a polygenic disease and the genes of GAMMA-interferon-inducible protein, insulin-like growth factor binding protein may play an important role in its pathogenesis.
Zheng
Shixin
S
Department of Otolaryngology, Peking University Shenzhen Hospital, Shenzhen, 518036, China.
Guo
Lianrong
L
Yao
Li
L
Liu
Jun
J
Zhang
Ruinv
R
Cai
Zhi
Z
Su
Yongjin
Y
D000328:Adult; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D055815:Young Adult