title: Differentiation of postnatal neural stem cells into glia and functional neurons on laminin-coated polymeric substrates.
abstract: A series of polymeric biomaterials, including poly(methyl acrylate), chitosan, poly(ethyl acrylate) (PEA), poly(hydroxyethyl acrylate) (PHEA), and a series of random copolymers containing ethyl acrylate, hydroxyethyl acrylate, and methyl acrylate were tested in vitro as culture substrates and compared for their effect on the differentiation of neural stem cells (NSCs) obtained from the subventricular zone of postnatal rats. Immunocytochemical assay for specific markers and scanning electron microscopy techniques were employed to determine the adhesion of the cultured NSCs to the different biomaterials and the respective neuronal differentiation. The functional properties and the membrane excitability of differentiated NSCs were investigated using a patch-clamp. The results show that the substrate's surface chemistry influences cell attachment and neuronal differentiation, probably through its influence on adsorbed laminin, and that copolymers based on PEA and PHEA in a narrow composition window are suitable substrates to promote cell attachment and differentiation of adult NSCs into functional neurons and glia.
authors.affiliation: Fundación Hospital General Universitario de Valencia, Valencia, Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martínez-Ramos
authors.forename: Sergio
authors.initials: S
authors.lastname: Lainez
authors.forename: Francisco
authors.initials: F
authors.lastname: Sancho
authors.forename: M Angeles
authors.initials: MA
authors.lastname: García Esparza
authors.forename: Rosa
authors.initials: R
authors.lastname: Planells-Cases
authors.forename: José Manuel
authors.initials: JM
authors.lastname: García Verdugo
authors.forename: José Luis
authors.initials: JL
authors.lastname: Gómez Ribelles
authors.forename: Manuel
authors.initials: M
authors.lastname: Salmerón Sánchez
authors.forename: Manuel
authors.initials: M
authors.lastname: Monleón Pradas
authors.forename: Juan Antonio
authors.initials: JA
authors.lastname: Barcia
authors.forename: José Miguel
authors.initials: JM
authors.lastname: Soria
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001672:Biocompatible Materials / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D002462:Cell Membrane / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004594:Electrophysiology; D007797:Laminin / Q000494:pharmacology*; D018625:Microscopy, Atomic Force; D009457:Neuroglia / Q000166:cytology* / Q000187:drug effects; D009474:Neurons / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D011102:Polyhydroxyethyl Methacrylate / Q000031:analogs & derivatives / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology*; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure
chemical_list: D001672:Biocompatible Materials; D007797:Laminin; D011108:Polymers; D011102:Polyhydroxyethyl Methacrylate; C023037:poly(2-hydroxyethyl acrylate)