title: Authentication of the traditional medicinal plant Eleutherococcus senticosus by DNA and chemical analyses.
abstract: Shigoka (SGK), the rhizome of Eleutherococcus senticosus, is a traditional medicine used as a tonic in northeastern Asia and far eastern Russia. We analyzed the nuclear ribosomal DNA internal transcribed spacer (ITS) sequence of the medicine available on the Japanese and Chinese markets and found that at least 3 species were used as the source plant of the commercial SGKs and that only 70% of all samples was made from the correct species. Furthermore, we performed the quantitative determination of 3 marker compounds, eleutheroside B (EB), syringaresinol diglucoside (Syr), and isofraxidin (Iso) by ultraperformance liquid chromatography (UPLC)/mass spectrometry (MS). We found that EB and Iso are specific to the correct source plant of SGK. Of them, EB is thought to be the best marker compound for quality assurance of the SGK from the viewpoint of its pharmacological activity.
authors.affiliation: Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, Setagaya-ku, Tokyo, Japan.
authors.forename: Takuro
authors.initials: T
authors.lastname: Maruyama
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kamakura
authors.forename: Miho
authors.initials: M
authors.lastname: Miyai
authors.forename: Katsuko
authors.initials: K
authors.lastname: Komatsu
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kawasaki
authors.forename: Masao
authors.initials: M
authors.lastname: Fujita
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimada
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Yamamoto
authors.forename: Toshiro
authors.initials: T
authors.lastname: Shibata
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Goda
mesh_terms: D003374:Coumarins / Q000032:analysis; D021903:DNA, Ribosomal Spacer; D028001:Eleutherococcus / Q000737:chemistry / Q000235:genetics*; D005960:Glucosides / Q000032:analysis; D017705:Lignans / Q000032:analysis; D010666:Phenylpropionates / Q000032:analysis; D028321:Plant Preparations / Q000737:chemistry / Q000592:standards; D010946:Plants, Medicinal / Q000737:chemistry / Q000235:genetics*; D027343:Rhizome / Q000737:chemistry / Q000235:genetics
chemical_list: D003374:Coumarins; D021903:DNA, Ribosomal Spacer; D005960:Glucosides; D017705:Lignans; D010666:Phenylpropionates; D028321:Plant Preparations; C407309:syringaresinol-4''-O-glucoside; C008182:isofraxidin; C028305:syringin