title: Reduction of surface roughness of a laminin-apatite composite coating via inhibitory effect of magnesium ions on apatite crystal growth.
abstract: An artificial material coated with a laminin-apatite composite layer on its surface would be useful as a percutaneous device with cell-adhesion properties and good biocompatibility. The present authors recently prepared such a composite by a liquid-phase coating process in which a surface-modified material was immersed in a laminin-containing calcium phosphate solution (LCP solution) supersaturated with respect to apatite. As a result of this coating process, however, the material increased in surface roughness, which may have an adverse effect on the prevention of bacterial infection. In the present study, a reduction of the surface roughness of the laminin-apatite composite layer was attempted by adding MgSO(4) to the LCP solution as an inhibitor of apatite crystal growth. The surface roughness, Ra, of the resulting laminin-apatite composite layer decreased from approximately 0.43 to 0.31mum upon increasing the MgSO(4) concentration in the LCP solution from 0 to 1.5mM, while retaining its cell adhesion characteristics. The thus-obtained material, having a laminin-apatite composite coating with reduced surface roughness, has potential as a material for percutaneous devices with improved resistance to bacterial infection through the interface between the device and the skin.
authors.affiliation: Nanotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 4, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan. a-oyane@aist.go.jp
authors.forename: Ayako
authors.initials: A
authors.lastname: Oyane
authors.forename: Yuusuke
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Yamazaki
authors.forename: Yu
authors.initials: Y
authors.lastname: Sogo
authors.forename: Katsuko
authors.initials: K
authors.lastname: Furukawa
authors.forename: Takashi
authors.initials: T
authors.lastname: Ushida
authors.forename: Atsuo
authors.initials: A
authors.lastname: Ito
mesh_terms: D001031:Apatites / Q000737:chemistry*; D002448:Cell Adhesion / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D003460:Crystallization / Q000379:methods*; D004847:Epithelial Cells / Q000187:drug effects / Q000502:physiology*; D006801:Humans; D007477:Ions; D007797:Laminin / Q000737:chemistry* / Q000494:pharmacology*; D008274:Magnesium / Q000737:chemistry*; D008422:Materials Testing; D013499:Surface Properties
chemical_list: D001031:Apatites; D020099:Coated Materials, Biocompatible; D007477:Ions; D007797:Laminin; D008274:Magnesium