Disposition and tissue distribution of ML12 in rats.
To investigate the disposition and tissue distribution of ML12 after intravenous (iv) administration in rats, the compound in plasma or in tissue was extracted into ethyl acetate under basic condition and was determined by HPLC after extracted by dilute sulfuric acid. Excitation wavelength and emission wavelength of fluorescence detection were 278 nm and 307 nm, respectively. The data were processed with the software 3P97 to calculate the main pharmaceutical parameters of ML12. At dose of 5 and 10 mg/kg, the elimination of the drug from plasma was found to be kinetically linear, but when the dosage was 20 mg/kg, a non-linear feature was observed. The highest level of ML12 was found in the kidney. Distribution of ML12 after iv administration was extensive and the concentration-time profile was found to be fitted to an open two-compartment model.
Si
Luqin
L
Department of Pharmaceutics, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. slq007@163.com
Li
Gao
G
Wu
Sanlan
S
Huang
Jiangeng
J
Wu
Dapeng
D
Gan
Quan
Q
D000818:Animals; D000959:Antihypertensive Agents / Q000493:pharmacokinetics*; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005260:Female; D007700:Kinetics; D008297:Male; D004364:Pharmaceutical Preparations; D051381:Rats; D017207:Rats, Sprague-Dawley; D013050:Spectrometry, Fluorescence / Q000379:methods; D013464:Sulfuric Acids / Q000737:chemistry; D014018:Tissue Distribution
D000959:Antihypertensive Agents; D004364:Pharmaceutical Preparations; D013464:Sulfuric Acids; C033158:sulfuric acid