Peripartal feeding strategy with different n-6:n-3 ratios in sows: effect on gene expression in backfat white adipose tissue postpartum.
The aim of this study was to describe the effects of two diets differing in n-6:n-3 ratio and prepartal feeding regime on gene expression of PPARgamma1a/1b, PPARgamma1c/1d, PPARgamma2, PPARgamma coactivator 1A (PPARGC1A), GLUT4, TNFalpha, adiponectin, leptin, leptin receptor (LEPR), fatty acid binding protein 4 (FABP4), lipoprotein lipase (LPL) in sows' white adipose tissue on the first day of lactation. The relationship between mRNA expression of these genes and circulating insulin, leptin and thyroid hormones was also considered. Diets contained a low (supplemented with fish oil; f group) or a high (supplemented with sunflower oil; s group) n-6:n-3 ratio and were provided from 8 (f8, s8) or 3d (f3, s3) before parturition (onset day 8 or 3). A low n-6:n-3 ratio reduced the 1d postpartum expression of PPARgamma2 and PPARGC1A but only when applied from 3 d before parturition. Circulating leptin was negatively correlated with mRNA expression of adiponectin, LEPR and LPL, whereas thyroxine was positively correlated with levels of PPARGC1A. In conclusion, the effect of dietary treatments, e.g. altering the n-6:n-3 ratio, around parturition on the expression of crucial genes in nutrient metabolism can be modulated by the duration of application before parturition.
Papadopoulos
Georgios A
GA
Laboratory of Animal Nutrition, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, B-9820 Merelbeke, Belgium. georgios.papadopoulos@ugent.be
Erkens
Tim
T
Maes
Dominiek G D
DG
Peelman
Luc J
LJ
van Kempen
Theo A T G
TA
Buyse
Johan
J
Janssens
Geert P J
GP
D052242:Adiponectin / Q000235:genetics; D052436:Adipose Tissue, White / Q000378:metabolism*; D000818:Animals; D001483:Base Sequence; D001786:Blood Glucose / Q000032:analysis; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage*; D043371:Fatty Acids, Omega-6 / Q000008:administration & dosage*; D005260:Female; D015870:Gene Expression; D051275:Glucose Transporter Type 4 / Q000235:genetics; D007328:Insulin / Q000097:blood; D007774:Lactation / Q000502:physiology*; D020738:Leptin / Q000097:blood / Q000235:genetics; D008071:Lipoprotein Lipase / Q000235:genetics; D008969:Molecular Sequence Data; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism; D011247:Pregnancy; D012333:RNA, Messenger / Q000032:analysis; D011897:Random Allocation; D054411:Receptors, Leptin / Q000235:genetics; D013552:Swine / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
D052242:Adiponectin; D001786:Blood Glucose; D015525:Fatty Acids, Omega-3; D043371:Fatty Acids, Omega-6; D051275:Glucose Transporter Type 4; D007328:Insulin; D020738:Leptin; D047495:PPAR gamma; D012333:RNA, Messenger; D054411:Receptors, Leptin; D014409:Tumor Necrosis Factor-alpha; D008071:Lipoprotein Lipase