title: Nanofluidic biosensing for beta-amyloid detection using surface enhanced Raman spectroscopy.
abstract: Trace detection of the conformational transition of beta-amyloid peptide (Abeta) from a predominantly alpha-helical structure to beta-sheet could have a large impact in understanding and diagnosing Alzheimer's disease. We demonstrate how a novel nanofluidic biosensor using a controlled, reproducible surface enhanced Raman spectroscopy active site was developed to observe Abeta in different conformational states during the Abeta self-assembly process as well as to distinguish Abeta from confounder proteins commonly found in cerebral spinal fluid.
authors.affiliation: Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843-3120, USA.
authors.forename: I-Hsien
authors.initials: IH
authors.lastname: Chou
authors.forename: Melodie
authors.initials: M
authors.lastname: Benford
authors.forename: Hope T
authors.initials: HT
authors.lastname: Beier
authors.forename: Gerard L
authors.initials: GL
authors.lastname: Coté
authors.forename: Miao
authors.initials: M
authors.lastname: Wang
authors.forename: Nan
authors.initials: N
authors.lastname: Jing
authors.forename: Jun
authors.initials: J
authors.lastname: Kameoka
authors.forename: Theresa A
authors.initials: TA
authors.lastname: Good
mesh_terms: D016229:Amyloid beta-Peptides / Q000032:analysis*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D013059:Spectrum Analysis, Raman / Q000295:instrumentation* / Q000379:methods; D020349:Surface Plasmon Resonance / Q000295:instrumentation* / Q000379:methods
chemical_list: D016229:Amyloid beta-Peptides