title: Treatment of chronical myocardial ischemia by adenovirus-mediated hepatocyte growth factor gene transfer in minipigs.
abstract: Growth factor gene transfer-induced therapeutic angiogenesis has become a novel approach for the treatment of myocardial ischemia. In order to provide a basis for the clinical application of an adenovirus with hepatocyte growth factor gene (Ad-HGF) in the treatment of myocardial ischemia, we established a minipig model of chronically ischemic myocardium in which an Ameroid constrictor was placed around the left circumflex branch of the coronary artery (LCX). A total of 18 minipigs were randomly divided into 3 groups: a surgery control group, a model group and an Ad-HGF treatment group implanted with Ameroid constrictor. Ad-HGF or the control agent was injected directly into the ischemic myocardium, and an improvement in heart function and blood supply were evaluated. The results showed that myocardial perfusion remarkably improved in the Ad-HGF group compared with that in both the control and model groups. Four weeks after the treatment, the density of newly formed blood vessels was higher and the number of collateral blood vessels was greater in the Ad-HGF group than in the model group. The area of myocardial ischemia reduced evidently and the left ventricular ejection fraction improved significantly in the Ad-HGF group. These results suggest that HGF gene therapy may become a novel approach in the treatment of chronically ischemic myocardium.
authors.affiliation: The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China. yuan.biao@live.cn
authors.forename: Biao
authors.initials: B
authors.lastname: Yuan
authors.forename: YouRong
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhao
authors.forename: DanLi
authors.initials: D
authors.lastname: Wu
authors.forename: LiZhen
authors.initials: L
authors.lastname: Yuan
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.forename: LiSheng
authors.initials: L
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Huang
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D004452:Echocardiography; D005260:Female; D015316:Genetic Therapy; D005822:Genetic Vectors; D017228:Hepatocyte Growth Factor / Q000235:genetics*; D008297:Male; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging / Q000628:therapy*; D013552:Swine; D013556:Swine, Miniature; D015899:Tomography, Emission-Computed, Single-Photon
chemical_list: D017228:Hepatocyte Growth Factor