title: The reversion effect of the RNAi-silencing mdr1 gene on multidrug resistance of the leukemia cell HT9.
abstract: Overexpression of P-glycoprotein (P-gp), the mdr1 gene product, confers multidrug resistance (MDR) to tumor cells and often limits the efficacy of chemotherapy. This study evaluated RNAi for specific silencing of the mdr1 gene and reversion of multidrug resistance. Three different short hairpin RNAs (shRNAs) were designed and constructed in a pSilencer 3.1-H1 neo plasmid. The shRNA recombinant plasmids were transfected into HT9 leukemia cells. The RNAi effect was evaluated by real-time PCR, Western blotting and cell cytotoxicity assay. In the cell, shRNAs can specifically down-regulate the expression of mdr1, mRNA and P-gp. Resistance against harringtonine, doxorubicin and curcumin was decreased. The study indicated that shRNA recombinant plasmids could modulate MDR in vitro.
authors.affiliation: College of Life Science, Beijing Normal University, 19 Xinjiekouwai Street, Beijing 100875, China. shaoshuli2008@163.com
authors.forename: Shu-Li
authors.initials: SL
authors.lastname: Shao
authors.forename: Ying-Yu
authors.initials: YY
authors.lastname: Sun
authors.forename: Xu-Yan
authors.initials: XY
authors.lastname: Li
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Fu
authors.forename: Dong-Ze
authors.initials: DZ
authors.lastname: Yun
authors.forename: Ming-Xue
authors.initials: MX
authors.lastname: Zuo
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D018432:Drug Resistance, Multiple; D019008:Drug Resistance, Neoplasm; D005822:Genetic Vectors; D018922:HL-60 Cells; D006801:Humans; D007938:Leukemia / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D014162:Transfection
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1