title: Characteristic comparison of triglyceride-rich remnant lipoprotein measurement between a new homogenous assay (RemL-C) and a conventional immunoseparation method (RLP-C).
abstract: BACKGROUND
  Increased serum remnant lipoproteins are supposed to predict cardiovascular disease in addition to increased LDL. A new homogenous assay for remnant lipoprotein-cholesterol (RemL-C) has been developed as an alternative to remnant-like particle-cholesterol (RLP-C), an immunoseparation assay, widely used for the measurement of remnant lipoprotein cholesterol.
  
  
  METHODS
  We evaluated the correlations and data validation between the 2 assays in 83 subjects (49 men and 34 women) without diabetes, hypertension and medications for hyperlipidemia, diabetes, and hypertension, and investigated the characteristics of remnant lipoproteins obtained by the two methods (RLP-C and RemL-C) and their relationships with IDL-cholesterol determined by our developed HPLC method.
  
  
  RESULTS
  A positive correlation was significantly found between the two methods (r = 0.853, 95%CI 0.781-0.903, p < 0.0001). Bland & Altman analysis revealed that RemL-C values were likely to be significantly higher than RLP-C values, particularly in samples with high levels of remnant lipoproteins. Several data dissociations between the RemL-C and RLP-C were also observed. The HPLC chromatograms show high concentrations of chylomicron cholesterol in serum samples with RemL-C level < RLP-C level, but high concentrations of IDL-cholesterol in samples with RemL-C level > RLP-C level. RemL-C (r = 0.339, 95%CI 0.152-0.903; p = 0.0005) significantly correlated with IDL-cholesterol, but not RLP-C (r = 0.17, 95%CI -0.047-0.372; p = 0.1237) in all the samples (n = 83).
  
  
  CONCLUSIONS
  These results suggest that there is generally a significant correlation between RemL-C and RLP-C. However, RemL-C assay is likely to reflect IDL more closely than RLP-C.
authors.affiliation: Department of Laboratory Medicine, Jikei University Kashiwa Hospital, Japan. hyoshida@jikei.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yoshida
authors.forename: Hideo
authors.initials: H
authors.lastname: Kurosawa
authors.forename: Yuji
authors.initials: Y
authors.lastname: Hirowatari
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Ogura
authors.forename: Katsunori
authors.initials: K
authors.lastname: Ikewaki
authors.forename: Ikuro
authors.initials: I
authors.lastname: Abe
authors.forename: Shinichi
authors.initials: S
authors.lastname: Saikawa
authors.forename: Kenichi
authors.initials: K
authors.lastname: Domitsu
authors.forename: Kumie
authors.initials: K
authors.lastname: Ito
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Yanai
authors.forename: Norio
authors.initials: N
authors.lastname: Tada
mesh_terms: D000368:Aged; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D002624:Chemistry, Clinical / Q000295:instrumentation / Q000379:methods; D002784:Cholesterol / Q000032:analysis* / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D019411:Clinical Laboratory Techniques / Q000295:instrumentation*; D005260:Female; D006801:Humans; D008074:Lipoproteins / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D015203:Reproducibility of Results; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008074:Lipoproteins; D014280:Triglycerides; C030104:lipoprotein cholesterol; C112017:remnant-like particle cholesterol; D002784:Cholesterol