Effect of cranberry juice concentrate on chemically-induced urinary bladder cancers.
The chemopreventive efficacy of cranberry juice concentrate in an experimental model of urinary bladder cancer was evaluated using female Fischer-344 rats. The animals received N-butyl-N-(4-hydroxybutyl)-nitrosamine (OH-BBN) for a period of eight weeks. Cranberry juice concentrate was administered at doses of 1.0 or 0.5 ml/rat/day beginning one week after the final OH-BBN treatment and continuing until the end of the study. The urinary bladders of all the rats were weighed and examined grossly for lesions, and all masses were submitted for pathological evaluation. A dose-dependent preventive effect of cranberry treatment was observed, with a reduced number of urinary bladder cancers (38%) in the 1.0 ml/rat/day group versus the control group. The cranberry extract neither affected body weight gain nor caused other signs of toxicity. For the metabolic studies, serum and urine were collected at 4 and 12 h after the administration of the cranberry juice concentrate and were analyzed by LC-MS/MS. Quercetin and its methylated derivative were detected in the urine samples. However, no quercetin was detected in the serum samples, indicating its poor bioavailability. These data suggest that components of cranberries may be effective in preventing urinary bladder carcinogenesis.
Prasain
Jeevan K
JK
Department of Pharmacology and Toxicology, University of Alabama, Birmingham, AL 35294-0019, USA.
Jones
Kenneth
K
Moore
Ray
R
Barnes
Stephen
S
Leahy
Marge
M
Roderick
Robin
R
Juliana
M Margaret
MM
Grubbs
Clinton J
CJ
D000818:Animals; D002085:Butylhydroxybutylnitrosamine; D002853:Chromatography, Liquid; D005260:Female; D008517:Phytotherapy; D010936:Plant Extracts / Q000627:therapeutic use*; D051381:Rats; D011916:Rats, Inbred F344; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D029799:Vaccinium macrocarpon / Q000737:chemistry*
D010936:Plant Extracts; D002085:Butylhydroxybutylnitrosamine