title: Complexins facilitate neurotransmitter release at excitatory and inhibitory synapses in mammalian central nervous system.
abstract: Complexins (Cplxs) are key regulators of synaptic exocytosis, but whether they act as facilitators or inhibitors is currently being disputed controversially. We show that genetic deletion of all Cplxs expressed in the mouse brain causes a reduction in Ca(2+)-triggered and spontaneous neurotransmitter release at both excitatory and inhibitory synapses. Our results demonstrate that at mammalian central nervous system synapses, Cplxs facilitate neurotransmitter release and do not simply act as inhibitory clamps of the synaptic vesicle fusion machinery.
authors.affiliation: Departments of Neuroscience and Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
authors.forename: Mingshan
authors.initials: M
authors.lastname: Xue
authors.forename: Alicja
authors.initials: A
authors.lastname: Stradomska
authors.forename: Hongmei
authors.initials: H
authors.lastname: Chen
authors.forename: Nils
authors.initials: N
authors.lastname: Brose
authors.forename: Weiqi
authors.initials: W
authors.lastname: Zhang
authors.forename: Christian
authors.initials: C
authors.lastname: Rosenmund
authors.forename: Kerstin
authors.initials: K
authors.lastname: Reim
mesh_terms: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; D000818:Animals; D001921:Brain / Q000378:metabolism / Q000502:physiology; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D005071:Evoked Potentials; D005089:Exocytosis / Q000235:genetics; D005136:Eye Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D009473:Neuronal Plasticity; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D018377:Neurotransmitter Agents / Q000378:metabolism*; D013569:Synapses / Q000378:metabolism*; D009435:Synaptic Transmission; D013572:Synaptic Vesicles / Q000187:drug effects / Q000378:metabolism
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D033942:Adaptor Proteins, Vesicular Transport; C529624:Cplx3 protein, mouse; D005136:Eye Proteins; D009419:Nerve Tissue Proteins; D018377:Neurotransmitter Agents; C095992:complexin I; C095993:complexin II; D002118:Calcium