title: Production of L(+)-lactic acid using acid-adapted precultures of Rhizopus arrhizus in a stirred tank reactor.
abstract: Cultivations of filamentous fungi in stirred tank reactors (STRs) to produce metabolites are often limited by insufficient mixing and mass transfer because of the formation of mycelial clumps inside the reactors. This study developed an acid-adapted preculture approach to control the morphology of filamentous Rhizopus arrhizus in a STR, consequently to enhance the production yield and productivity of L(+)-lactic acid efficiently using waste potato starch as substrate. Using the acid-adapted precultures as inoculum, the morphology of R. arrhizus was maintained as large clumps, coalesced loose small pellets, and freely dispersed small pellets. The highest lactic acid concentration of 85.7 g/L with a yield of 86% was obtained in association with the formation of coalesced loose small pellets. The results indicate that the use of the acid-adapted precultures as inoculum is a promising approach for lactic acid production in STRs.
authors.affiliation: School of Earth and Environmental Sciences, The University of Adelaide, Adelaide, SA 5005, Australia.
authors.forename: Zhan Ying
authors.initials: ZY
authors.lastname: Zhang
authors.forename: Bo
authors.initials: B
authors.lastname: Jin
authors.forename: Joan M
authors.initials: JM
authors.lastname: Kelly
mesh_terms: D000143:Acids; D003470:Culture Media; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D019344:Lactic Acid / Q000096:biosynthesis*; D012233:Rhizopus / Q000378:metabolism*
chemical_list: D000143:Acids; D003470:Culture Media; D019344:Lactic Acid