title: Combined Raman spectroscopy and optical coherence tomography device for tissue characterization.
abstract: We report a dual-modal device capable of sequential acquisition of Raman spectroscopy (RS) and optical coherence tomography (OCT) along a common optical axis. The device enhances application of both RS and OCT by precisely guiding RS acquisition with OCT images while also compensating for the lack of molecular specificity in OCT with the biochemical specificity of RS. We characterize the system performance and demonstrate the capability to identify structurally ambiguous features within an OCT image with RS in a scattering phantom, guide acquisition of RS from a localized malignancy in ex vivo breast tissue, and perform in vivo tissue analysis of a scab.
authors.affiliation: Department of Biomedical Engineering, Vanderbilt University, Station B Box 351631, Nashville, Tennessee 37235, USA. c.patil@vanderbilt.edu
authors.forename: Chetan A
authors.initials: CA
authors.lastname: Patil
authors.forename: Nienke
authors.initials: N
authors.lastname: Bosschaart
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Keller
authors.forename: Ton G
authors.initials: TG
authors.lastname: van Leeuwen
authors.forename: Anita
authors.initials: A
authors.lastname: Mahadevan-Jansen
mesh_terms: D001940:Breast / Q000166:cytology*; D001943:Breast Neoplasms / Q000473:pathology*; D005260:Female; D005385:Fingers / Q000473:pathology; D006801:Humans; D007090:Image Interpretation, Computer-Assisted; D019047:Phantoms, Imaging; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods; D041623:Tomography, Optical Coherence / Q000295:instrumentation / Q000379:methods; D014947:Wounds and Injuries / Q000473:pathology