[Cloning, expression and radiation inducibility of RadA from the hyperthermophilic archaeon Sulfolobus tokodaii].
RecA/Rad51/RadA recombinases are important recombination proteins with conserved functions. Studies on the enzymes have shown that members of RecA/Rad51/RadA family from bacteria, eukaryota, methanogens and halophilic archaea have UV inducibility. However, the UV inducibility of RadA homologues from hyperthermophilic archaea is controversial. We analyzed the UV inducibility of Sulfolobus tokodaii RadA by RT-PCR and immune assays. Comparing with the mock, the transcription and expression of the radA increased 2 and 1.5 folds respectively after UV irradiation at 100 J/m2, or 3 and 1 fold at 200 J/m2. These results demonstrated that S. tokodaii RadA could be induced after UV treatment. In addition, proteome induction analysis proved that there existed a DNA damage induction response in S. tokodaii, which further supported RadA inductility in this hyperthermophilic archaeon.
Sheng
Duohong
D
State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. dhsheng@sdu.edu.cn
Zhu
Shanshan
S
Li
Mingfeng
M
Jiao
Jiandong
J
Ni
Jinfeng
J
Shen
Yulong
Y
D000906:Antibodies / Q000032:analysis / Q000276:immunology; D019843:Archaeal Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D003001:Cloning, Molecular; D004249:DNA Damage; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D015180:Electrophoresis, Gel, Two-Dimensional; D019848:Gene Expression Regulation, Archaeal / Q000528:radiation effects*; D007118:Immunoassay; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016944:Sulfolobus / Q000235:genetics* / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects
D000906:Antibodies; D019843:Archaeal Proteins; D004268:DNA-Binding Proteins; C117252:RadA protein, archaeal