Highly sensitive biosensor based on bionanomultilayer with water-soluble multiwall carbon nanotubes for determination of phenolics.
A highly sensitive biosensor was developed based on bionanomultilyer with water-soluble carbon nanotubes (CNTs). The water-soluble poly(allylamine hydrochloride)-wrapped multiwall carbon nanotubes (PAH-MWNTs) can be obtained for the first time relying on the function of barbiturates, which provides a useful avenue for CNT application in material science and biosensor technology. Based on this, the PAH-MWNTs/horseradish peroxidase (HRP) bionanomultilayer was prepared via layer-by-layer (LBL) assembly. Electrochemical impedance spectroscopy, atomic force microscopy and UV-vis spectra were adopted to monitor the uniform LBL assembly of the homogeneous bionanomultilayer. The bionanomultilayer was used to construct a phenolic biosensor. Under the optimal conditions, the biosensor presented a linear response for catechol from 0.1 to 20.4 microM, with a detection limit of 0.06 microM. A series of phenolics were detected by the bionanomultilayer biosensor. The introduced MWNTs in the biosensor provided a suitable microenvironment to retain the HRP activity and acted as a transducer for amplifying the electrochemical signal of the product of the enzymatic reaction. So the developed bionanomultilayer biosensor exhibited a fast, sensitive and stable detection.
Liu
Lijun
L
Department of Chemistry, Zhejiang University, Hangzhou 310027, PR China.
Zhang
Fu
F
Xi
Fengna
F
Lin
Xianfu
X
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006735:Horseradish Peroxidase / Q000737:chemistry*; D008839:Microelectrodes; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012995:Solubility; D014867:Water / Q000737:chemistry*
D037742:Nanotubes, Carbon; D010636:Phenols; D014867:Water; D006735:Horseradish Peroxidase