An electrochemiluminescent biosensor for uric acid based on the electrochemiluminescence of bis-[3,4,6-trichloro-2-(pentyloxycarbonyl)-phenyl] oxalate on an ITO electrode modified by an electropolymerized nickel phthalocyanine film.
Nickel(ii) tetrasulfophthalo-cyanine (NiTSPc) could be electrodeposited onto an indium tin oxide (ITO) electrode to form an electropolymerization film of NiTSPc. The electrochemiluminescent (ECL) behavior of bis-[3,4,6-trichloro-2-(pentyloxy-carbonyl)-phenyl] oxalate (BTPPO) on this modified electrode was investigated in detail. The emission of ECL of BTPPO can be greatly enhanced by hydrogen peroxide at this modified electrode. Thus, a new ECL biosensor for uric acid was developed based on the enzymatic reaction of uric acid in the presence of uricase to produce H(2)O(2). The developed sensor has been used to detect uric acid in real serum samples, and the results compared well with those obtained by the routine method.
Lin
Zhenyu
Z
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety (Fuzhou University) and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China.
Chen
Zhihuang
Z
Liu
Yan
Y
Wang
Jian
J
Chen
Guonan
G
D015374:Biosensing Techniques; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006801:Humans; D006861:Hydrogen Peroxide; D007211:Indoles; D054833:Isoindoles; D008163:Luminescent Measurements; D009532:Nickel; D011108:Polymers; D017971:Tin Compounds; D014527:Uric Acid / Q000097:blood*
D007211:Indoles; D054833:Isoindoles; D011108:Polymers; D017971:Tin Compounds; D014527:Uric Acid; C109984:indium tin oxide; D009532:Nickel; D006861:Hydrogen Peroxide; C013647:phthalocyanine