Current status, strategies, and potential for the metabolic engineering of heterologous polyketides in Escherichia coli.
Heterologous natural product biosynthesis has emerged as a strategy to produce medicinal compounds that pose challenges to conventional production routes. Polyketide compounds, an important class of natural products with wide-ranging therapeutic value, have been heterologously produced through Escherichia coli, presenting new opportunities to realize the medicinal potential of polyketide natural products. However, current production levels are often suboptimal when compared to native strain producers or heterologous theoretical yields. This problem provides an excellent opportunity to apply and further develop current metabolic engineering tools.
Boghigian
Brett A
BA
Department of Chemical and Biological Engineering Science and Technology Center, Tufts University, 4 Colby Street, Medford, MA 02155, USA.
Pfeifer
Blaine A
BA
D004926:Escherichia coli / Q000378:metabolism*; D005818:Genetic Engineering / Q000379:methods* / Q000639:trends*; D018942:Macrolides / Q000378:metabolism*; D048630:Polyketide Synthases / Q000096:biosynthesis
D018942:Macrolides; D048630:Polyketide Synthases