title: Endothelin-1 production by normal human cultured keratinocytes and its regulation.
abstract: The possibility that cultured keratinocytes produce endothelins were investigated. The results showed that cultured keratinocytes derived from normal human skin produce endothelin-1. Moreover, keratinocyte endothelin-1 production was completely inhibited by the presence of actinomycin D in the medium. As in the case of endothelial cells, recombinant interleukin-1beta was capable of promoting endothelin-1 production in keratinocytes, whereas herapin inhibited it. Thrombin also inhibited endothelin-1 production. These results indicate that the mechanism of endothelin-1 production in keratinocytes is slightly different from the mechanism in vascular endothelial cells.
authors.affiliation: Department of Plastic and Reconstructive Surgery St Marianna University School of Medicine 2-16-1 Sugao Miyamae Kawasaki 216 Japan.
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: N
authors.initials: N
authors.lastname: Wakisaka
authors.forename: N
authors.initials: N
authors.lastname: Tane
authors.forename: K
authors.initials: K
authors.lastname: Ando
authors.forename: E
authors.initials: E
authors.lastname: Isono
authors.forename: M
authors.initials: M
authors.lastname: Yamanaka
authors.forename: M
authors.initials: M
authors.lastname: Aihara
authors.forename: H
authors.initials: H
authors.lastname: Ishida