title: Detection of the administration of 17beta-nortestosterone in boars by gas chromatography/mass spectrometry.
abstract: 17beta-Nortestosterone (17betaN) is illegally used in livestock as a growth promoter and its endogenous production has been described in some animals, such as adult boars. In this paper, the metabolism of 17betaN in boars has been studied by gas chromatography/mass spectrometry (GC/MS) in order to identify markers of the exogenous administration. Administration studies of intramuscular 17betaN laurate to male pigs were performed. Free, sulphate and glucuronide fractions of the urine samples were separated and the steroids present were quantified by GC/MS. 17betaN was detected in some pre-administration samples. After administration, 17betaN, norandrosterone, noretiocholanolone (NorE), norepiandrosterone, 5beta-estrane-3alpha,17beta-diol and 5alpha-estrane-3beta,17beta-diol were detected in different fractions, being the most important metabolites, 17betaN excreted as a sulphate and free NorE. Samples collected in routine controls were also analyzed by GC/MS to identify endogenous compounds. 17betaN, norandrostenedione and estrone were detected in almost all the samples. No other 17betaN metabolites were detected. According to these results, the detection by GC/MS of some of the 17betaN metabolites described above, different from 17betaN, could be indicative of the exogenous administration of 17betaN to boars.
authors.affiliation: Unitat de Recerca en Farmacologia, Institut Municipal d'Investigació Mèdica, IMIM, Barcelona, Spain. rventura@imim.es
authors.forename: Rosa
authors.initials: R
authors.lastname: Ventura
authors.forename: Meritxell
authors.initials: M
authors.lastname: Roig
authors.forename: Belén
authors.initials: B
authors.lastname: Pérez
authors.forename: Sergi
authors.initials: S
authors.lastname: López
authors.forename: Mireia
authors.initials: M
authors.lastname: Medina
authors.forename: Jaume
authors.initials: J
authors.lastname: Bosch
authors.forename: Jordi
authors.initials: J
authors.lastname: Segura
mesh_terms: D045930:Anabolic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000652:urine; D000818:Animals; D005240:Feasibility Studies; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D020719:Glucuronides / Q000737:chemistry; D008297:Male; D015394:Molecular Structure; D009277:Nandrolone / Q000008:administration & dosage* / Q000737:chemistry / Q000652:urine; D012015:Reference Standards; D052616:Solid Phase Extraction; D015813:Substance Abuse Detection / Q000379:methods*; D013431:Sulfates / Q000737:chemistry; D013552:Swine
chemical_list: D045930:Anabolic Agents; D020719:Glucuronides; D013431:Sulfates; D009277:Nandrolone