[Evaluation of glycosaminoglycans of periurethral tissue in patients with and without pelvic organ prolapse].
OBJECTIVE
To characterize and quantify periurethral tissue sulphated glycosaminoglycans (GAGs) in women with and without pelvic organ prolapse.


METHODS
Periurethral tissue was obtained from 35 women who underwent surgery for pelvic organ prolapse, for stress urinary incontinence, or for other gynecological benign conditions. Patients were submitted to a clinical history, physical and urodynamic examination and were divided in two groups according to genital prolapse. The standard biopsy with 1.0 x 1.0 cm was taken from periurethral tissue during surgery and assessed by biochemical methods. The GAGs were obtained by proteolysis and precipitated by trichloroacetic acid. The relative concentration of sulfated GAGs was determined by densitometry of toluidine blue stained gel using a spectrophotometer with a 525 nm wavelength. Data were compared using analysis of variance (ANOVA).


RESULTS
In the two groups dermatan sulphate (DS) was the predominant glycosaminoglycan (85%), followed by chondroitin sulphate (CS) and heparan sulphate (HS). Women with pelvic organ prolapse had significantly more total GAGs, DS and HS. Differences in CS were not observed.


CONCLUSIONS
This study showed altered biochemical characteristics in the extracellular matrix of periurethral tissue and also accumulation of GAGs, DS and CS, in women with pelvic organ prolapse.
Feldner
Paulo Cezar
PC
Uroginecologia e Cirurgia Vaginal do Departamento de Ginecologia da Universidade Federal de São Paulo (UNIFESP/EPM). pfel@dneralfa.epm.br
Kobayashi
Elsa Yoko
EY
Sartori
Marair Gracio Ferreira
MG
Nader
Helena Bonciani
HB
Baracat
Edmund Chada
EC
Girão
Manoel João Batista Castello
MJ
D000328:Adult; D000368:Aged; D000704:Analysis of Variance; D002809:Chondroitin Sulfates / Q000032:analysis / Q000378:metabolism; D003871:Dermatan Sulfate / Q000032:analysis / Q000378:metabolism; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis* / Q000378:metabolism; D006801:Humans; D008875:Middle Aged; D014521:Urethra / Q000737:chemistry* / Q000378:metabolism; D014550:Urinary Incontinence, Stress / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D014596:Uterine Prolapse / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D055815:Young Adult
D006025:Glycosaminoglycans; D003871:Dermatan Sulfate; D002809:Chondroitin Sulfates