Plant volatiles activating specific olfactory receptor neurons of the cabbage moth Mamestra brassicae L. (Lepidoptera, Noctuidae).
Herbivore insects are suitable model organisms for studying how plant odor information is encoded in olfactory receptor neurons (RNs). By the use of gas chromatography linked to electrophysiological recordings from single RNs, screening for sensitivity to naturally produced plant odorants is possible in order to determine the molecular receptive ranges of the neurons. Using this method, we have in this study of the cabbage moth, Mamestra brassicae, classified 21 types of olfactory RNs according to their responses to odorants present in the host plants of Brassicae, in the related species of Arabidopsis, as well as in essential oils of nonhost plants like ylang-ylang. Most of the RNs were tuned to one or a few structurally similar compounds, showing minimal overlap of their molecular receptive ranges. Whereas some RNs displayed a novel tuning, others were tuned to the same compounds as neurons in other insect species. We also found colocation in the same sensillum of 3 RN types with the same response characteristics and tuning as 3 colocated types described in heliothine moths living on different host plants. The presence of similar RN types across different insect species implies conservation or reappearance of the RN types, independent of the evolution of host plant ranges.
Ulland
S
S
Neuroscience Unit, Department of Biology, Norwegian University of Science and Technology, NO-7489 Trondheim, Norway.
Ian
E
E
Stranden
M
M
Borg-Karlson
A-K
AK
Mustaparta
H
H
D000818:Animals; D002849:Chromatography, Gas; D003485:Cyanates / Q000737:chemistry / Q000494:pharmacology; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D009036:Moths / Q000187:drug effects* / Q000378:metabolism* / Q000648:ultrastructure; D018034:Olfactory Receptor Neurons / Q000378:metabolism*; D010944:Plants / Q000737:chemistry*; D013729:Terpenes / Q000737:chemistry / Q000494:pharmacology; D014835:Volatilization
D003485:Cyanates; D005227:Fatty Acids; D013729:Terpenes