title: Role of mitogen-activated protein kinase (MAPK) docking sites on Staufen2 protein in dendritic mRNA transport.
abstract: Although transport and subsequent translation of dendritic mRNA play an important role in neuronal synaptic plasticity, the underlying mechanisms for modulating dendritic mRNA transport are almost completely unknown. In this study, we identified and characterized an interaction between Staufen2 and mitogen-activated protein kinase (MAPK) with co-immunoprecipitation assays. Staufen2 utilized a docking (D) site to interact with ERK1/2; deleting the D-site decreased colocalization of Staufen2 with immunoreactive ERK1/2 in the cell body regions of cultured hippocampal neurons, and it reduced the amount of Staufen2-containing RNP complexes in the distal dendrites. In addition, the deletion completely abolished the depolarization-induced increase of Staufen2-containing RNP complexes. These results suggest that the MAPK pathway could modulate dendritic mRNA transport through its interaction with Staufen2.
authors.affiliation: Department of Medicine and Microbiology, College of Medicine, Signaling Disorder Research Center, Chungbuk National University, Sungbong-ro 410, Cheongju 361-763, Republic of Korea.
authors.forename: Yeon-Ju
authors.initials: YJ
authors.lastname: Nam
authors.forename: Hyo-Soon
authors.initials: HS
authors.lastname: Cheon
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Choi
authors.forename: Seok-Yong
authors.initials: SY
authors.lastname: Kim
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Shin
authors.forename: Eung-Gook
authors.initials: EG
authors.lastname: Kim
authors.forename: Hyong Kyu
authors.initials: HK
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D003712:Dendrites / Q000378:metabolism*; D006801:Humans; D047468:Immunoprecipitation; D051379:Mice; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism*; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism*; D008969:Molecular Sequence Data; D009473:Neuronal Plasticity; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017384:Sequence Deletion
chemical_list: D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C492188:Stau2 protein, rat; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3