[Effects of quercetin on human laryngeal cancer Hep-2 cells].
OBJECTIVE
To investigate the antiproliferative effects of quercetinon on human laryngeal cancer Hep-2 cells and its mechanism.


METHODS
The antiproliferative effects of quercetinon on human laryngeal cancer Hep-2 cells was evaluated by MTT assay. The morphological alterations were studied by inverted phase contrast microscope. The apoptosis and cell cycle of Hep-2 cells was analyzed by flow cytometry (FCW).


RESULTS
The growth of Hep-2 cells was significantly inhibited by quercetin. By inverted phase contrast microscope, morphological alterations including adherencing capability weakening and shape shrinking were noticed. It was found that apoptosis cells increased with the increase of drug concentration and the elapse of time by FCW. Cell cycle transition arrested at S phase and the broken DNA was noticed.


CONCLUSIONS
Quercetin could effectively inhibit the proliferation of Hep-2 cells and its mechanism is probably related to the apoptosis.
Liu
Rongyao
R
Department of Otolaryngology-Head and Neck Surgery, Renji Hospital, Shanghai Jiaotong University, Shanghai, 200001,China.
Wang
Jiadong
J
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology; D011794:Quercetin / Q000494:pharmacology*
D000970:Antineoplastic Agents; D011794:Quercetin