title: Prevalence and sequence diversity of a factor required for actin-based motility in natural populations of Burkholderia species.
abstract: Actin-based motility of the melioidosis pathogen Burkholderia pseudomallei requires BimA. We report a high degree of conservation of bimA in 99 B. pseudomallei isolates from the area of endemicity. A geographically restricted subset of B. pseudomallei isolates harbored a B. mallei-like bimA allele (12.1%), confounding a differential diagnostic test based on amplification of species-specific bimA regions.
authors.affiliation: Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, 2 Pran-nok Road, Bangkoknoi, Bangkok 10700, Thailand.
authors.forename: Chayada
authors.initials: C
authors.lastname: Sitthidet
authors.forename: Joanne M
authors.initials: JM
authors.lastname: Stevens
authors.forename: Narisara
authors.initials: N
authors.lastname: Chantratita
authors.forename: Bart J
authors.initials: BJ
authors.lastname: Currie
authors.forename: Sharon J
authors.initials: SJ
authors.lastname: Peacock
authors.forename: Sunee
authors.initials: S
authors.lastname: Korbsrisate
authors.forename: Mark P
authors.initials: MP
authors.lastname: Stevens
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D042726:Burkholderia mallei / Q000235:genetics; D016957:Burkholderia pseudomallei / Q000235:genetics* / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D019353:Endemic Diseases; D006801:Humans; D008124:Locomotion; D008554:Melioidosis / Q000453:epidemiology / Q000382:microbiology; D008840:Microfilament Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: C503173:BimA protein, Burkholderia pseudomallei; D004269:DNA, Bacterial; D008840:Microfilament Proteins