title: Early forebrain wiring: genetic dissection using conditional Celsr3 mutant mice.
abstract: Development of axonal tracts requires interactions between growth cones and the environment. Tracts such as the anterior commissure and internal capsule are defective in mice with null mutation of Celsr3. We generated a conditional Celsr3 allele, allowing regional inactivation. Inactivation in telencephalon, ventral forebrain, or cortex demonstrated essential roles for Celsr3 in neurons that project axons to the anterior commissure and subcerebral targets, as well as in cells that guide axons through the internal capsule. When Celsr3 was inactivated in cortex, subcerebral projections failed to grow, yet corticothalamic axons developed normally, indicating that besides guidepost cells, additional Celsr3-independent cues can assist their progression. These observations provide in vivo evidence that Celsr3-mediated interactions between axons and guidepost cells govern axonal tract formation in mammals.
authors.affiliation: Developmental Neurobiology, Université Catholique de Louvain, 1200 Bruxelles, Belgique.
authors.forename: Libing
authors.initials: L
authors.lastname: Zhou
authors.forename: Isabelle
authors.initials: I
authors.lastname: Bar
authors.forename: Younès
authors.initials: Y
authors.lastname: Achouri
authors.forename: Kenneth
authors.initials: K
authors.lastname: Campbell
authors.forename: Olivier
authors.initials: O
authors.lastname: De Backer
authors.forename: Jean M
authors.initials: JM
authors.lastname: Hebert
authors.forename: Kevin
authors.initials: K
authors.lastname: Jones
authors.forename: Nicoletta
authors.initials: N
authors.lastname: Kessaris
authors.forename: Catherine Lambert
authors.initials: CL
authors.lastname: de Rouvroit
authors.forename: Dennis
authors.initials: D
authors.lastname: O'Leary
authors.forename: William D
authors.initials: WD
authors.lastname: Richardson
authors.forename: Andre M
authors.initials: AM
authors.lastname: Goffinet
authors.forename: Fadel
authors.initials: F
authors.lastname: Tissir
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology*; D015820:Cadherins / Q000235:genetics* / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology; D005260:Female; D020868:Gene Silencing; D020772:Internal Capsule / Q000166:cytology / Q000196:embryology / Q000502:physiology; D008297:Male; D051379:Mice; D009434:Neural Pathways / Q000196:embryology* / Q000502:physiology; D009474:Neurons / Q000502:physiology*; D016548:Prosencephalon / Q000166:cytology / Q000196:embryology* / Q000502:physiology; D011956:Receptors, Cell Surface / Q000235:genetics* / Q000502:physiology*; D012686:Septal Nuclei / Q000196:embryology / Q000502:physiology; D013788:Thalamus / Q000166:cytology / Q000196:embryology; D046509:Tissue Culture Techniques
chemical_list: D015820:Cadherins; C444454:Celsr3 protein, mouse; D011956:Receptors, Cell Surface