title: Quantitative determination of oil content in small quantity of oilseed rape by ultrasound-assisted extraction combined with gas chromatography.
abstract: Accurately quantitative determination of oil content in oilseed rape plays an important role in varieties breeding for improving oil content in seeds. However, large quantity of oilseeds were needed in order to obtain accuracy and precision results by using standard Soxhlet extraction method, which may be a handicap in analysis of small, rare and precious samples in plant breeding. In the present work, ultrasound-assisted extraction was evaluated as a simpler and more effective alternative to conventional extraction method for the isolation of oil from small quantity of oilseed rape (<20 mg). The oil of oilseed rape samples was extracted by ultrasound-assisted method, and then the fatty acids and total oil content of the seeds were qualitatively and quantitatively determined by gas chromatography (GC). Extraction efficiency of total oil obtained by ultrasound-assisted extraction through an orthogonal experiment (L(9) (3(4))) were investigated to get the best extraction conditions. Statistical analysis showed that the variable with the largest effect was the ultrasound-assisted extraction time which was followed by the ultrasound-assisted extraction power, and the liquid:solid ratio. A liquid:solid ratio of 1:4 (L:g), an ultrasound-assisted extraction time of 60 min and an ultrasound-assisted extraction power of 500 W were found to be optimal for oil extraction from oilseed rape. By comparing with the conventional method, it was found that the ultrasound-assisted extraction of oil from oilseed rape was about five times faster than the traditional extraction method. By the use of ultrasound-assisted extraction combined with GC analysis, the fatty acids and total oil content in small quantity of seeds (<20 mg) were successfully qualitatively determined and the results are in agreement with that obtained by traditional standard method.
authors.affiliation: Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, PR China. willasa@163.com
authors.forename: Fang
authors.initials: F
authors.lastname: Wei
authors.forename: Gui-Zhen
authors.initials: GZ
authors.lastname: Gao
authors.forename: Xin-Fa
authors.initials: XF
authors.lastname: Wang
authors.forename: Xu-Yan
authors.initials: XY
authors.lastname: Dong
authors.forename: Ping-Ping
authors.initials: PP
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.forename: Xu
authors.initials: X
authors.lastname: Wang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Wu
authors.forename: Hong
authors.initials: H
authors.lastname: Chen
mesh_terms: D000704:Analysis of Variance; D029741:Brassica rapa / Q000737:chemistry* / Q000528:radiation effects; D002849:Chromatography, Gas; D005227:Fatty Acids / Q000032:analysis; D005229:Fatty Acids, Monounsaturated; D007202:Indicators and Reagents; D010938:Plant Oils / Q000032:analysis* / Q000528:radiation effects; D000074262:Rapeseed Oil; D014465:Ultrasonics
chemical_list: D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D007202:Indicators and Reagents; D010938:Plant Oils; D000074262:Rapeseed Oil