[Correlative study of nuclear factor-kappa B subunit p50 activity and IL-4 gamma-IFN expression in nasal polyps].
OBJECTIVE
To explore the possible regulation mechanism of nuclear factor-kappa B subunit p50 on Th1/Th2 cytokine in nasal polyps.


METHODS
The expression of IL-4, gamma-IFN and p50 were examined by the method of immunohistochemistry staining in 40 cases of nasal polyps and 20 normal subjects, and the linear correlations between activity of p50 and IL-4, gamma-IFN were evaluated.


RESULTS
The expression of p50 mainly distribute in the cytoplasm of epithelia, inflammatory cell and the epithelia of gland. The expression of IL-4 and the activity of p50 was increased significantly in nasal polyps (all P<0.01). But the expression of gamma-IFN had no significant difference between the groups (P>0.05). Pearson correlation analysis demonstrated that p50 nuclear-presented closely correlation with IL-4 level, but not gamma-IFN. The correlation coefficient was 0.70 for p50 and IL-4 (P<0.01), 0.14 for p50 and gamma-IFN (P>0.05).


CONCLUSIONS
The activity of p50 in nasal polyps is increased significantly. The activation of p50 may play an important role in the imbalance of Th1/Th2-typed cytokines by upregulating the level of IL-4.
Li
Yunqiu
Y
Department of Otolaryngology, Hunan Province People's Hospital, Changsha, 410002, China. entliyunqiu@126.com
Feng
Yong
Y
Wang
Jihua
J
Xiao
Xuping
X
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D009298:Nasal Polyps / Q000378:metabolism* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics; D055815:Young Adult
C508598:IL4 protein, human; D052002:NF-kappa B p50 Subunit; D012333:RNA, Messenger; D015847:Interleukin-4; D007371:Interferon-gamma