Detection of the administration of 17beta-nortestosterone in boars by gas chromatography/mass spectrometry.
17beta-Nortestosterone (17betaN) is illegally used in livestock as a growth promoter and its endogenous production has been described in some animals, such as adult boars. In this paper, the metabolism of 17betaN in boars has been studied by gas chromatography/mass spectrometry (GC/MS) in order to identify markers of the exogenous administration. Administration studies of intramuscular 17betaN laurate to male pigs were performed. Free, sulphate and glucuronide fractions of the urine samples were separated and the steroids present were quantified by GC/MS. 17betaN was detected in some pre-administration samples. After administration, 17betaN, norandrosterone, noretiocholanolone (NorE), norepiandrosterone, 5beta-estrane-3alpha,17beta-diol and 5alpha-estrane-3beta,17beta-diol were detected in different fractions, being the most important metabolites, 17betaN excreted as a sulphate and free NorE. Samples collected in routine controls were also analyzed by GC/MS to identify endogenous compounds. 17betaN, norandrostenedione and estrone were detected in almost all the samples. No other 17betaN metabolites were detected. According to these results, the detection by GC/MS of some of the 17betaN metabolites described above, different from 17betaN, could be indicative of the exogenous administration of 17betaN to boars.
Ventura
Rosa
R
Unitat de Recerca en Farmacologia, Institut Municipal d'Investigació Mèdica, IMIM, Barcelona, Spain. rventura@imim.es
Roig
Meritxell
M
Pérez
Belén
B
López
Sergi
S
Medina
Mireia
M
Bosch
Jaume
J
Segura
Jordi
J
D045930:Anabolic Agents / Q000008:administration & dosage* / Q000737:chemistry / Q000652:urine; D000818:Animals; D005240:Feasibility Studies; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D020719:Glucuronides / Q000737:chemistry; D008297:Male; D015394:Molecular Structure; D009277:Nandrolone / Q000008:administration & dosage* / Q000737:chemistry / Q000652:urine; D012015:Reference Standards; D052616:Solid Phase Extraction; D015813:Substance Abuse Detection / Q000379:methods*; D013431:Sulfates / Q000737:chemistry; D013552:Swine
D045930:Anabolic Agents; D020719:Glucuronides; D013431:Sulfates; D009277:Nandrolone