Protein plasticity: a single amino acid substitution in the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase generates protosta-13(17),24-dien-3beta-ol, a rearrangement product.
To provide insights into the structure-function relationships of oxidosqualene-lanosterol cyclase (ERG7) from Saccharomyces cerevisiae, the Phe699 was exchanged against hydrophilic polar uncharged residues Ser, Thr, Cys, Gln, and Tyr to characterize its product profile and functional role in ERG7 activity. Among the substitutions, only the ERG7(F699T) mutant produced novel protosta-13(17),24-dien-3beta-ol as the sole truncated rearrangement product. The results suggest that Phe699Thr mutation is likely to affect the C-17 cation stabilization during the rearrangement process.
Wu
Tung-Kung
TK
Department of Biological Science and Technology, Institute of Molecular Science, National Chiao Tung University, Hsin-Chu, Taiwan, ROC. tkwmll@mail.nctu.edu.tw
Wen
Hao-Yu
HY
Chang
Cheng-Hsiang
CH
Liu
Yuan-Ting
YT
D019943:Amino Acid Substitution; D000596:Amino Acids / Q000235:genetics; D018360:Crystallography, X-Ray; D019751:Intramolecular Transferases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009154:Mutation; D010649:Phenylalanine / Q000737:chemistry / Q000235:genetics; D011487:Protein Conformation; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D013329:Structure-Activity Relationship; D013912:Threonine / Q000737:chemistry / Q000235:genetics; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
D000596:Amino Acids; D014315:Triterpenes; C530466:protosta-13(17),24-dien-3beta-ol; D013912:Threonine; D010649:Phenylalanine; D019751:Intramolecular Transferases; C021924:lanosterol synthase