The microarray analysis for gene expression in haploids and diploids derived from twin-seedling rice.
In this study, microarray technique was employed to analyze the gene expression at the RNA level between haploids and corresponding diploids derived from a rice twin-seedling line SARII-628. Different degrees of expression variations were observed in the plant after haploidization. The main results are as follows: (1) after haploidization, the ratio of the sensitive loci was 2.47% of the total loci designed on chip. Those loci were randomly distributed on the 12 pairs of rice chromosomes and the activated loci were more than the silenced ones. (2) Gene clusters on chromosome were observed for 33 sequences. (3) GoPipe function classification for 575 sensitive loci revealed an involvement in the biological process, cell component and molecular function. (4) RT-PCR generally validated the result from microarray with a coincidence rate of 83.78%. And for the randomly-selected activated or silenced loci in chip analysis, the coincidence rate was up to 91.86%.
Zhang
HongYu
H
Rice Research Institute of Sichuan Agricultural University, Chengdu 611130, China.
Peng
Hai
H
Li
PingChuan
P
Deng
QiMing
Q
Xu
PeiZhou
P
Li
Yun
Y
Wang
XuDong
X
Wu
XianJun
X
D004171:Diploidy; D015870:Gene Expression; D006238:Haploidy; D020411:Oligonucleotide Array Sequence Analysis; D012275:Oryza / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction