Presence of Panulirus argus virus 1 (PaV1) in juvenile spiny lobsters Panulirus argus from the Caribbean coast of Mexico.
Macroscopic evidence, histological sections, transmission electron microscopy (TEM) evaluation, and PCR analyses of 25 apparently diseased juvenile spiny lobsters Panulirus argus from the reef lagoon of Puerto Morelos, Mexico, showed the presence of Panulirus argus Virus 1 (PaV1). Cowdry Type A intranuclear viral inclusions were observed in histological analyses, icosahedral viral particles were observed by TEM, and PCR using specific primers for PaV1 amplified a fragment of 499 bp. This is the first report of PaV1 infecting P. argus outside the Florida Keys, USA.
Huchin-Mian
Juan Pablo
JP
Laboratorio de Inmunología y Biologá Molecular, Centro de Investigaci6n y de Estudios Avanzados del Instituto Politécnico Nacional, Unidad Mérida, Km. 6 Antigua Carretera a Progreso, CORDEMEX, M6rida, Yucatan 97310, México.
Rodríguez-Canul
Rossanna
R
Arias-Bañuelos
Efrain
E
Simá-Alvarez
Raúl
R
Pérez-Vega
Juan A
JA
Briones-Fourzán
Patricia
P
Lozano-Alvarez
Enrique
E
D000818:Animals; D017691:Caribbean Region; D043143:Hepatopancreas / Q000821:virology; D008800:Mexico; D033521:Palinuridae / Q000821:virology*; D016133:Polymerase Chain Reaction; D014780:Viruses / Q000302:isolation & purification* / Q000648:ultrastructure