Bacterial cellulose production by Gluconacetobacter sp. PKY5 in a rotary biofilm contactor.
A rotary biofilm contactor (RBC) inoculated with Gluconacetobacter sp. RKY5 was used as a bioreactor for improved bacterial cellulose production. The optimal number of disk for bacterial cellulose production was found to be eight, at which bacterial cellulose and cell concentrations were 5.52 and 4.98 g/L. When the aeration rate was maintained at 1.25 vvm, bacterial cellulose and cell concentrations were maximized (5.67 and 5.25 g/L, respectively). The optimal rotation speed of impeller in RBC was 15 rpm. When the culture pH in RBC was not controlled during fermentation, the maximal amount of bacterial cellulose (5.53 g/L) and cells (4.91 g/L) was obtained. Under the optimized culture conditions, bacterial cellulose and cell concentrations in RBC reached to 6.17 and 5.58 g/L, respectively.
Kim
Yong-Jun
YJ
Department of Material Chemical and Biochemical Engineering, Chonnam National University, 500-757, Gwangju, Korea.
Kim
Jin-Nam
JN
Wee
Young-Jung
YJ
Park
Don-Hee
DH
Ryu
Hwa-Won
HW
D018441:Biofilms / Q000254:growth & development*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation; D002482:Cellulose / Q000378:metabolism*; D020576:Gluconobacter / Q000254:growth & development* / Q000378:metabolism*; D012399:Rotation
D002482:Cellulose