title: Participation of Rho, ROCK-2, and GAP activities during actin microfilament rearrangements in Entamoeba histolytica induced by fibronectin signaling.
abstract: In Entamoeba histolytica little is known about the microfilament rearrangements formed by actin and ABPs. Fibronectin regulates many aspects of cell behavior involving the actin cytoskeleton and members of the Rho family of small GTPases. Using trophozoites interacted with fibronectin and glass, we present evidence related with the formation and regulation of different microfilament rearrangements and their cellular distribution, the effect of actin affecting drugs on these arrangements, and on trophozoites adhesion; we also demonstrate that actin isoforms are induced after adhesion, and also the selective participation of specific actin binding proteins such as ABP-120 and phospho-paxillin, regarding their location in the different actin structures. In addition, we show results that confirm the participation of EhRho, ROCK-2, and GAP activities. We propose that fibronectin induced signaling in E. histolytica trophozoites have important consequences in the actin cytoskeleton that may affect its behavior during the invasive process in the host.
authors.affiliation: Departamento de Patología Experimental, CINVESTAV-IPN, Avenida IPN No. 2508, Col. San Pedro Zacatenco, México, D.F. 07360, Mexico.
authors.forename: Amelia
authors.initials: A
authors.lastname: Ríos
authors.forename: Verónica Ivonne
authors.initials: VI
authors.lastname: Hernández-Ramírez
authors.forename: Mayra
authors.initials: M
authors.lastname: Moguel
authors.forename: Alma Iris
authors.initials: AI
authors.lastname: Zárate Bahena
authors.forename: José Luis
authors.initials: JL
authors.lastname: Rosales-Encina
authors.forename: Miguel Angel
authors.initials: MA
authors.lastname: Vargas
authors.forename: Patricia
authors.initials: P
authors.lastname: Talamás-Rohana
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism* / Q000648:ultrastructure; D000199:Actins / Q000378:metabolism; D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000494:pharmacology; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism; D047630:Depsipeptides / Q000494:pharmacology; D004748:Entamoeba histolytica / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D005353:Fibronectins / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D006801:Humans; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D020033:Protein Isoforms / Q000378:metabolism; D015398:Signal Transduction; D053778:Thiazolidines / Q000494:pharmacology; D053058:Trophozoites / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D020741:rho GTP-Binding Proteins / Q000378:metabolism*; D054460:rho-Associated Kinases / Q000378:metabolism*
chemical_list: D000199:Actins; D019086:Bridged Bicyclo Compounds, Heterocyclic; D047630:Depsipeptides; D005353:Fibronectins; D020690:GTPase-Activating Proteins; D019384:Nucleic Acid Synthesis Inhibitors; D020033:Protein Isoforms; D053778:Thiazolidines; C057531:jasplakinolide; D015638:Cytochalasin D; D054460:rho-Associated Kinases; D020741:rho GTP-Binding Proteins; C037067:latrunculin A