A simple and efficient method for deriving neurospheres from bone marrow stromal cells.
Bone marrow stromal cells (MSCs) can be differentiated into neuronal and glial-like cell types under appropriate experimental conditions. However, previously reported methods are complicated and involve the use of toxic reagents. Here, we present a simplified and nontoxic method for efficient conversion of rat MSCs into neurospheres that express the neuroectodermal marker nestin. These neurospheres can proliferate and differentiate into neuron, astrocyte, and oligodendrocyte phenotypes. We thus propose that MSCs are an emerging model cell for the treatment of a variety of neurological diseases.
Yang
Qin
Q
Department of Neurology, The First Affiliated Hospital, Chongqing Medical University, The Key Laboratory of Diagnostic Medicine Designated by the Ministry of Education, 1 Yixueyuan Road, Chongqing 400016, China.
Mu
Jun
J
Li
Qi
Q
Li
Ao
A
Zeng
Zhilei
Z
Yang
Jun
J
Zhang
Xiaodong
X
Tang
Jin
J
Xie
Peng
P
D000818:Animals; D001253:Astrocytes / Q000166:cytology; D015415:Biomarkers / Q000032:analysis; D015153:Blotting, Western; D001854:Bone Marrow Cells / Q000166:cytology*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D007150:Immunohistochemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009474:Neurons / Q000166:cytology; D009836:Oligodendroglia / Q000166:cytology; D051381:Rats; D017154:Stromal Cells / Q000166:cytology
D015415:Biomarkers