Effect of mineral trioxide aggregate on dentin bridge formation and expression of dentin sialoprotein and heme oxygenase-1 in human dental pulp.
This study was conducted to evaluate the pulpal response to direct capping with either mineral trioxide aggregate (MTA) or calcium hydroxide (CH) cement in humans, with a focus on dentin bridge formation and dentin sialoprotein (DSP) and heme oxygenase-1 (HO-1) expression. Direct pulp capping was performed in 20 cases of caries-free human third molars. The pulps were exposed and capped with either MTA or hard-setting CH. After 2 months, the teeth were extracted, and the specimens were prepared for histologic and immunohistochemical evaluations. Histologically, 100% of the MTA group and 60% of the CH group developed dentin bridges. The mean thickness of the dentin bridges observed in the MTA group was statistically greater than that of CH group. In addition, DSP and HO-1 were expressed in the odontoblast-like cells and pulp fibroblasts beneath the dentin bridge; furthermore, significantly greater immunostaining was observed in the MTA group than in the CH group. Collectively, these results indicate that MTA is superior to CH in terms of inducing the dentinogenic process in human pulp capping.
Min
Kyung-San
KS
Department of Conservative Dentistry, College of Dentistry, Wonkwang University, Iksan, Republic of Korea.
Park
Hyo-Jin
HJ
Lee
Sun-Kyung
SK
Park
Sang-Hyuk
SH
Hong
Chan-Ui
CU
Kim
Hae-Won
HW
Lee
Hae-Hyoung
HH
Kim
Eun-Cheol
EC
D000328:Adult; D017607:Aluminum Compounds / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D019610:Cytoprotection; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003785:Dental Pulp Capping / Q000379:methods*; D003809:Dentin, Secondary / Q000254:growth & development / Q000378:metabolism*; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000502:physiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D008964:Molar, Third; D009804:Odontoblasts / Q000378:metabolism; D010087:Oxides / Q000494:pharmacology*; D010750:Phosphoproteins; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012795:Sialoglycoproteins; D017640:Silicates / Q000494:pharmacology*; D000099311:Dentin Sialophosphoprotein
D017607:Aluminum Compounds; D017610:Calcium Compounds; D002126:Calcium Hydroxide; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D051547:Heme Oxygenase-1; D010087:Oxides; D010750:Phosphoproteins; D012387:Root Canal Filling Materials; D012795:Sialoglycoproteins; D017640:Silicates; D000099311:Dentin Sialophosphoprotein; C086631:mineral trioxide aggregate