title: Removal of estrogenic activity from endocrine-disrupting chemicals by purified laccase of Phlebia tremellosa.
abstract: A white-rot basidiomycete, Phlebia tremellosa, produced a laccase that showed increased activity during degradation of phthalates. A laccase was purified through the ion exchange chromatography and preparative gel electrophoresis, and the estimated molecular weight was 75 kDa. The optimum pH and temperature of the purified laccase was pH 4.0 and 20 degrees C, respectively. The K(m) value of the enzyme was 55.7 microM, and the V(max) was 0.0541 OD min(-1) U(-1) for o-tolidine. Purified laccase reduced the estrogenic activity of four different endocrine-disrupting chemicals. However, this effect was reduced by a laccase inhibitor, kojic acid, which confirmed that the laccase was involved in the removal of estrogenic activity.
authors.affiliation: Molecular Microbiology Laboratory, Department of Biochemistry, Division of Life Sciences, and Research Institute of Life Sciences, Kangwon National University, Chunchon, Korea.
authors.forename: Yunjung
authors.initials: Y
authors.lastname: Kim
authors.forename: Sumin
authors.initials: S
authors.lastname: Yeo
authors.forename: Myung K
authors.initials: MK
authors.lastname: Kim
authors.forename: Hyoung T
authors.initials: HT
authors.lastname: Choi
mesh_terms: D001487:Basidiomycota / Q000201:enzymology*; D001560:Benzidines / Q000378:metabolism; D002852:Chromatography, Ion Exchange; D004591:Electrophoresis, Polyacrylamide Gel; D052244:Endocrine Disruptors / Q000378:metabolism*; D004967:Estrogens / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000302:isolation & purification* / Q000378:metabolism*; D008970:Molecular Weight; D010636:Phenols / Q000378:metabolism; D010795:Phthalic Acids / Q000378:metabolism; D011753:Pyrones / Q000378:metabolism; D013696:Temperature
chemical_list: D001560:Benzidines; D052244:Endocrine Disruptors; D004967:Estrogens; D010636:Phenols; D010795:Phthalic Acids; D011753:Pyrones; C009501:2-tolidine; C011890:kojic acid; D042845:Laccase