Characterization of KlGUT2, a gene of the glycerol-3-phosphate shuttle, in Kluyveromyces lactis.
KlGUT2 encodes the mitochondrial component of the glycerol-3-phosphate shuttle in Kluyveromyces lactis, a dehydrogenase involved in the maintenance of the NADH redox balance and in glycerol utilization. Deletion of KlGUT2 led to glycerol accumulation during growth in glucose and growth retardation in ethanol. In addition, KlGUT2 deletion altered the expression of other mitochondrial dehydrogenases that contribute to the maintenance of the intracellular redox balance, suggesting a rerouting of ethanol oxidation from the cytoplasm to the mitochondria. Finally, Northern analysis showed that KlGUT2 has two transcripts: one constitutively expressed and dependent on HGT1, the high-affinity hexose transporter gene, and the other induced under respiratory conditions.
Saliola
Michele
M
Department of Cell and Developmental Biology, University of Rome La Sapienza, Piazzale Aldo Moro, Rome, Italy. michele.saliola@uniroma1.it
Sponziello
Marialuisa
M
D'Amici
Sirio
S
Lodi
Tiziana
T
Falcone
Claudio
C
D015152:Blotting, Northern; D000431:Ethanol / Q000378:metabolism; D017353:Gene Deletion; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D005947:Glucose / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D007716:Kluyveromyces / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D008928:Mitochondria / Q000201:enzymology; D008954:Models, Biological; D016254:Mutagenesis, Insertional; D009243:NAD / Q000378:metabolism; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000096:biosynthesis; D012331:RNA, Fungal / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis
D012331:RNA, Fungal; D012333:RNA, Messenger; D009243:NAD; D000431:Ethanol; D010088:Oxidoreductases; D005993:Glycerolphosphate Dehydrogenase; C096203:glycerol-3-phosphate dehydrogenase (NAD(P)+); D005947:Glucose; D005990:Glycerol