title: Enhancement of rhamnoplipid production in residual soybean oil by an isolated strain of Pseudomonas aeruginosa.
abstract: In the present work, the production of rhamnolipid from residual soybean oil (RSO) from food frying facilities was studied using a strain of Pseudomonas aeruginosa of contaminated lagoon, isolated from a hydrocarbon contaminated soil. The optimization of RSO, ammonium nitrate, and brewery residual yeast concentrations was accomplished by a central composite experimental design and surface response analysis. The experiments were performed in 500-mL Erlenmeyer flasks containing 50 mL of mineral medium, at 170 rpm and 30 +/- 1 degrees C, for a 48-h fermentation period. Rhamnolipid production has been monitored by measurements of surface tension, rhamnose concentration, and emulsifying activity. The best-planned results, located on the central point, have corresponded to 22 g/L of RSO, 5.625 g/L of NH(4)NO(3), and 11.5 g/L of brewery yeast. At the maximum point the values for rhamnose and emulsifying index were 2.2 g/L and 100%, respectively.
authors.affiliation: Universidade Federal de Uberlândia/FEQ, Caixa Postal 593, 38400-902, Uberlândia, MG, Brazil.
authors.forename: C J B
authors.initials: CJ
authors.lastname: de Lima
authors.forename: F P
authors.initials: FP
authors.lastname: França
authors.forename: E F C
authors.initials: EF
authors.lastname: Sérvulo
authors.forename: M M
authors.initials: MM
authors.lastname: Resende
authors.forename: V L
authors.initials: VL
authors.lastname: Cardoso
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D006017:Glycolipids / Q000378:metabolism*; D010865:Pilot Projects; D011550:Pseudomonas aeruginosa / Q000145:classification* / Q000378:metabolism*; D013024:Soybean Oil / Q000378:metabolism*; D013045:Species Specificity; D013501:Surface-Active Agents / Q000378:metabolism*
chemical_list: D006017:Glycolipids; D013501:Surface-Active Agents; C418382:rhamnolipid; D013024:Soybean Oil