title: Crotalphine, a novel potent analgesic peptide from the venom of the South American rattlesnake Crotalus durissus terrificus.
abstract: We have shown that the venom of the South American rattlesnake Crotalus durissus terrificus induces a long-lasting antinociceptive effect mediated by activation of kappa- and delta-opioid receptors. Despite being mediated by opioid receptors, prolonged treatment with the crotalid venom does not cause the development of peripheral tolerance or abstinence symptoms upon withdrawal. In the present study, we have isolated and chemically characterized a novel and potent antinociceptive peptide responsible for the oral opioid activity of this crotalid venom. The amino acid sequence of this peptide, designated crotalphine, was determined by mass spectrometry and corroborated by solid-phase synthesis to be <EFSPENCQGESQPC, where <E is pyroglutamic acid and the two cysteine residues forming a disulfide bond. This 14-amino-acid residue sequence is identical to the gamma-chain sequence of crotapotin, a non-toxic component of this snake venom. Crotalphine, when orally administered (0.008-25mug/kg), induces antinociceptive effect in the prostaglandin E(2)- and carrageenin-induced mechanical hyperalgesia models in rats and in the hot-plate test in mice. Crotalphine was also effective when administered by intravenous (0.0032-0.04mug/kg) or intraplantar (s.c., 0.00006-0.3mug/paw) routes. In the mechanical hyperalgesia models, crotalphine shows a long-lasting (5 days) antinociceptive effect. d-Phe-Cys-Tyr-d-Trp-Orn-Thr-Pen-Thr amide (CTOP) and N,N-diallyl-Tyr-Aib-Aib-Phe-Leu (ICI 174,864), antagonists of mu- and delta-opioid receptors, respectively, did not alter the antinociceptive effect of the peptide, whereas nor-binaltorphimine, an antagonist of kappa-opioid receptors, blocked this effect. These results indicate that crotalphine induces antinociception mediated by activation of kappa-opioid receptors and may contribute to the antinociceptive effect of the crotalid venom.
authors.affiliation: Center for Applied Toxinology, Butantan Institute, Avenida Vital Brazil, 1500 São Paulo, SP 05503-900, Brazil.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Konno
authors.forename: Gisele
authors.initials: G
authors.lastname: Picolo
authors.forename: Vanessa P
authors.initials: VP
authors.lastname: Gutierrez
authors.forename: Patrícia
authors.initials: P
authors.lastname: Brigatte
authors.forename: Vanessa O
authors.initials: VO
authors.lastname: Zambelli
authors.forename: Antonio C M
authors.initials: AC
authors.lastname: Camargo
authors.forename: Yara
authors.initials: Y
authors.lastname: Cury
mesh_terms: D000595:Amino Acid Sequence; D000700:Analgesics / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D003435:Crotalid Venoms / Q000737:chemistry*; D017839:Crotalus; D003439:Crotoxin / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D008297:Male; D008969:Molecular Sequence Data; D010147:Pain Measurement / Q000187:drug effects; D010455:Peptides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011913:Rats, Inbred BB; D013020:South America; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000700:Analgesics; D003435:Crotalid Venoms; D010455:Peptides; C533006:crotalphine; D003439:Crotoxin