title: Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding.
abstract: Broad-spectrum matrix metalloproteinase (MMP) inhibitors (MMPI) were unsuccessful in cancer clinical trials, partly due to side effects resulting from limited knowledge of the full repertoire of MMP substrates, termed the substrate degradome, and hence the in vivo functions of MMPs. To gain further insight into the degradome of MMP-14 (membrane type 1 MMP) an MMPI, prinomastat (drug code AG3340), was used to reduce proteolytic processing and ectodomain shedding in human MDA-MB-231 breast cancer cells transfected with MMP-14. We report a quantitative proteomic evaluation of the targets and effects of the inhibitor in this cell-based system. Proteins in cell-conditioned medium (the secretome) and membrane fractions with levels that were modulated by the MMPI were identified by isotope-coded affinity tag (ICAT) labeling and tandem mass spectrometry. Comparisons of the expression of MMP-14 with that of a vector control resulted in increased MMP-14/vector ICAT ratios for many proteins in conditioned medium, indicating MMP-14-mediated ectodomain shedding. Following MMPI treatment, the MMPI/vehicle ICAT ratio was reversed, suggesting that MMP-14-mediated shedding of these proteins was blocked by the inhibitor. The reduction in shedding or the release of substrates from pericellular sites in the presence of the MMPI was frequently accompanied by the accumulation of the protein in the plasma membrane, as indicated by high MMPI/vehicle ICAT ratios. Considered together, this is a strong predictor of biologically relevant substrates cleaved in the cellular context that led to the identification of many undescribed MMP-14 substrates, 20 of which we validated biochemically, including DJ-1, galectin-1, Hsp90alpha, pentraxin 3, progranulin, Cyr61, peptidyl-prolyl cis-trans isomerase A, and dickkopf-1. Other proteins with altered levels, such as Kunitz-type protease inhibitor 1 and beta-2-microglobulin, were not substrates in biochemical assays, suggesting an indirect affect of the MMPI, which might be important in drug development as biomarkers or, in preclinical phases, to predict systemic drug actions and adverse side effects. Hence, this approach describes the dynamic pattern of cell membrane ectodomain shedding and its perturbation upon metalloproteinase drug treatment.
authors.affiliation: Department of Oral Biological and Medical Sciences, Centre for Blood Research, University of British Columbia, Vancouver, Canada.
authors.forename: Georgina S
authors.initials: GS
authors.lastname: Butler
authors.forename: Richard A
authors.initials: RA
authors.lastname: Dean
authors.forename: Eric M
authors.initials: EM
authors.lastname: Tam
authors.forename: Christopher M
authors.initials: CM
authors.lastname: Overall
mesh_terms: D000595:Amino Acid Sequence; D001943:Breast Neoplasms / Q000201:enzymology* / Q000473:pathology; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects* / Q000201:enzymology*; D017077:Culture Media, Conditioned; D004354:Drug Screening Assays, Antitumor; D037483:Galectin 1 / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006801:Humans; D006877:Hydroxamic Acids / Q000494:pharmacology*; D036341:Intercellular Signaling Peptides and Proteins / Q000737:chemistry / Q000378:metabolism; D007553:Isotope Labeling; D053511:Matrix Metalloproteinase 14 / Q000378:metabolism*; D061965:Matrix Metalloproteinase Inhibitors; D008954:Models, Biological; D008969:Molecular Sequence Data; D015513:Oncogene Proteins / Q000378:metabolism; D009930:Organic Chemicals / Q000494:pharmacology; D000077153:Progranulins; D011480:Protease Inhibitors / Q000494:pharmacology*; D040901:Proteomics; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism; D015203:Reproducibility of Results; D013379:Substrate Specificity / Q000187:drug effects; D000094963:Axl Receptor Tyrosine Kinase
chemical_list: D017077:Culture Media, Conditioned; C492314:GRN protein, human; D037483:Galectin 1; D018841:HSP90 Heat-Shock Proteins; D006877:Hydroxamic Acids; D036341:Intercellular Signaling Peptides and Proteins; D061965:Matrix Metalloproteinase Inhibitors; D015513:Oncogene Proteins; D009930:Organic Chemicals; D000077153:Progranulins; D011480:Protease Inhibitors; D011518:Proto-Oncogene Proteins; C113282:prinomastat; D020794:Receptor Protein-Tyrosine Kinases; D053511:Matrix Metalloproteinase 14; D000094963:Axl Receptor Tyrosine Kinase; C000722289:AXL protein, human