Labeling embryonic mouse central nervous system cells by in utero electroporation.
During cerebral development, neurons are generated near the ventricle and then migrate toward the pial surface. In this review, we describe the method of in utero electroporation, this method allows the morphology of the migrating neurons to be visualized and the effect of overexpression or knock down of any gene to be examined. After electroporation of a green fluorescent protein (GFP) expression vector by this method, GFP-positive cells are first found in the ventricular zone, and their distribution then gradually shift toward the pial surface. A few days later, most of the GFP positive cells were aligned beneath the marginal zone, with the normal course of cortical neuronal migration.
Tabata
Hidenori
H
Department of Anatomy, Keio University School of Medicine, Tokyo, Japan.
Nakajima
Kazunori
K
D000818:Animals; D002490:Central Nervous System / Q000196:embryology*; D002540:Cerebral Cortex / Q000196:embryology*; D015509:Developmental Biology / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D046149:Embryo Culture Techniques / Q000379:methods*; D018014:Gene Transfer Techniques; D051379:Mice; D009420:Nervous System / Q000196:embryology*; D009474:Neurons / Q000378:metabolism; D010957:Plasmids / Q000378:metabolism; D013997:Time Factors