title: Label-free detection of sequence-specific DNA with multiwalled carbon nanotubes and their light scattering signals.
abstract: A detection method for DNA sequence-specificity in a homogeneous medium is presented with multiwalled carbon nanotubes (MWCNTs) as optical probes on the basis of the measurements of light scattering signals. ssDNA can prevent MWCNTs from coagulation in electrolyte solution while dsDNA cannot, displaying different light scattering signals. With the light scattering signals, target DNA in the range of 8.6-86.4 nM could be detected and one base pair mismatch could be discriminated. The sequence specificity for the present method has been identified with PCR products.
authors.affiliation: College of Chemistry and Chemical Engineering, MOE Key Laboratory of Luminescence and Real-Time Analysis, CQKL-LRTA, Southwest University, Chongqing, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.forename: Cheng Zhi
authors.initials: CZ
authors.lastname: Huang
authors.forename: Yuan Fang
authors.initials: YF
authors.lastname: Li
authors.forename: Sai Jin
authors.initials: SJ
authors.lastname: Xiao
authors.forename: Jian Ping
authors.initials: JP
authors.lastname: Xie
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D017931:DNA Primers; D008027:Light; D037742:Nanotubes, Carbon; D012542:Scattering, Radiation
chemical_list: D017931:DNA Primers; D037742:Nanotubes, Carbon; D004247:DNA