Transient silencing of Plasmodium falciparum Tudor Staphylococcal Nuclease suggests an essential role for the protein.
Plasmodium falciparum Tudor Staphylococcal Nuclease (PfTSN) has a multidomain organization and preferentially cleaves single stranded RNAs. PfTSN is quite distinct from its vertebrate homologues both in terms of its primary sequence and functional activity. Here, we analyzed the effect of PfTSN specific siRNA on parasite growth and development. Treatment of parasite culture with PfTSN siRNA at the late ring stage resulted in substantial inhibition in parasite growth. The PfTSN siRNA treated parasite cultures showed significant reduction in specific mRNA and PfTSN expression. Morphological examination of PfTSN siRNA treated parasites showed block in the development of parasite at the trophozoite stage. Treatment of parasites with a specific inhibitor of micrococcal nucleases, 3',5'-deoxythymidine biphosphate (pdTp) resulted in similar block in parasite development, thereby suggesting that PfTSN plays an important role at the trophozoite stage of the parasite. Collectively, our findings point towards an essential role for the PfTSN in the parasite's infection cycle.
Sunil
Sujatha
S
International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India.
Hossain
Manzar J
MJ
Ramasamy
Gowthaman
G
Malhotra
Pawan
P
D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006801:Humans; D008836:Micrococcal Nuclease / Q000235:genetics / Q000502:physiology*; D010963:Plasmodium falciparum / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D015800:Protozoan Proteins / Q000235:genetics / Q000502:physiology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D013942:Thymine Nucleotides / Q000494:pharmacology
D004791:Enzyme Inhibitors; D015800:Protozoan Proteins; D034741:RNA, Small Interfering; D013942:Thymine Nucleotides; C019111:thymidine 3',5'-diphosphate; D008836:Micrococcal Nuclease