Single-cell microinjection technology in cell biology.
Single-cell microinjection has been successfully used to deliver exogenous proteins, cDNA constructs, peptides, drugs and particles into transfection-challenged cells. With precisely controlled delivery dosage and timing, microinjection has been used in many studies of primary cultured cells, transgenic animal production, in vitro fertilization and RNA inference. This review discusses the advantages and limits of microinjection as a mechanical delivery method and its applications to attached and suspended cells.
Zhang
Yan
Y
Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China. yanzhang@pku.edu.cn
Yu
Long-Chuan
LC
D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation / Q000379:methods*; D005456:Fluorescent Dyes; D005821:Genetic Techniques / Q000295:instrumentation; D006801:Humans; D008845:Microinjections / Q000295:instrumentation; D009474:Neurons / Q000166:cytology; D014161:Transduction, Genetic
D005456:Fluorescent Dyes