[Effect of Bushen Antai Recipe on prostaglandin I2 expression and its nuclear receptor at implantation site in mice with blastocyst implantation dysfunction].
OBJECTIVE
To observe the effect of Bushen Antai Recipe (BAR) on expression of prostaglandin I2 (PGI2) and its nuclear receptor peroxisome proliferators-activated receptor delta (PPARdelta) at implantation site in mice with blastocyst implantation dysfunction.


METHODS
Pregnant mice were divided into three groups randomly, the normal group, the model group and the BAR group. The pregnant uterus of all mice was cut off on the 4th (D4), 5th (D5), 6th (D6) and 8th (D8) day of pregnancy for determining the PGI2 expression with radio immunoassay; and the mRNA and protein expression of PPARdelta with RT-PCR and immunohistochemistry at implantation site.


RESULTS
PGI2 expression in the model group was obviously lower than that in the normal group (P < 0.01), and also lower than that in the BAR group (P < 0.01), while the index was insignificantly different between the normal and the BAR group. Compared with the normal group, the expression of PPARdelta in the model group was delayed temporally and spatially (P < 0.05), while that in the BAR group was not significantly different.


CONCLUSIONS
BAR can improve the implantation in mice with blastocyst implantation dysfunction through promoting the PGI2 expression and its nuclear receptor PPARdelta at implantation site.
Zhang
Ming-Min
MM
Department of Integrated Traditional Chinese and Western Medicine, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan. mmzhang@tjh.tj-mu.edu.cn
Cheng
Liang-Liang
LL
Dong
Li-Ping
LP
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D010065:Embryo Implantation, Delayed / Q000187:drug effects*; D004717:Endometrium / Q000187:drug effects / Q000378:metabolism; D011464:Epoprostenol / Q000096:biosynthesis*; D005260:Female; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D047494:PPAR delta / Q000096:biosynthesis* / Q000235:genetics; D011247:Pregnancy; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011863:Radioimmunoassay; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004365:Drugs, Chinese Herbal; D047494:PPAR delta; D012333:RNA, Messenger; C534019:bushen antai; D011464:Epoprostenol