title: Column reversed-phase high-performance liquid chromatographic method for simultaneous determination of rabeprazole sodium and domperidone in combined tablet dosage form.
abstract: A new, simple column reversed-phase high-performance liquid chromatographic (HPLC) method for simultaneous determination of rabeprazole sodium (RAB) and domperidone (DOM) in a combined tablet dosage form has been developed and validated. Determination was performed using a Jasco HPLC system with a HiQ SiL octadecylsilane (C18) column (250 x 4.6 mm id), acetonitrile-0.1 M ammonium acetate (50 + 50, v/v) mobile phase, and paracetamol as an internal standard. The detection was performed using a UV detector set at 280 nm. The method was validated with respect to linearity, accuracy, precision, and robustness. Beer's law was obeyed in the concentration range of 1.0-10.0 and 0.5-5.0 microg/mL for RAB and DOM, respectively. The method has been successfully applied for the analysis of drugs in a pharmaceutical formulation.
authors.affiliation: All India Shri Shivagi Memorial Society's College of Pharmacy, Department of Pharmaceutical Analysis, Kennedy Rd, Pune 411001, Maharashtra, India.
authors.forename: Shweta Sadanand
authors.initials: SS
authors.lastname: Sabnis
authors.forename: Dhavale Nilesh
authors.initials: DN
authors.lastname: Dnvandev
authors.forename: Vijay Yeshawantrao
authors.initials: VY
authors.lastname: Jadhav
authors.forename: Santosh Vilashchand
authors.initials: SV
authors.lastname: Gandhi
mesh_terms: D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004294:Domperidone / Q000032:analysis*; D004338:Drug Combinations; D064750:Rabeprazole; D013607:Tablets
chemical_list: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D004338:Drug Combinations; D013607:Tablets; D064750:Rabeprazole; D004294:Domperidone