Practical method for detection and identification of Candida, Aspergillus, and Scedosporium spp. by use of rolling-circle amplification.
A sensitive rolling-circle amplification (RCA)-based method utilizing species-specific padlock probes targeted to the internal transcribed spacer 2 region of the fungal ribosomal DNA gene complex was developed. The assay was rapid (2 hours) and specific. Of 28 fungal isolates (16 of Candida, six of Aspergillus, and six of Scedosporium spp.), all were all identified correctly.
Zhou
Xiaoyong
X
Department of Dermatology, Research Laboratory for Infectious Skin Diseases, Wuhan First Hospital, Wuhan 430022, People's Republic of China.
Kong
Fanrong
F
Sorrell
Tania C
TC
Wang
Hui
H
Duan
Yiqun
Y
Chen
Sharon C A
SC
D001228:Aspergillosis / Q000382:microbiology; D001230:Aspergillus / Q000302:isolation & purification*; D002175:Candida / Q000302:isolation & purification*; D002177:Candidiasis / Q000382:microbiology; D004271:DNA, Fungal / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D006801:Humans; D008271:Mycetoma / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D015345:Oligonucleotide Probes / Q000235:genetics; D021681:Scedosporium / Q000302:isolation & purification*; D012680:Sensitivity and Specificity
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D015345:Oligonucleotide Probes