Development of a sensitive flow-injection-chemiluminescence detection method for the determination of levodopa.
A simple chemiluminometric method using flow injection has been developed for the determination of levodopa, based on its sensitizing effect on the weak chemiluminescence (CL) reaction between Na(2)SO(3) and acidic KMnO4. Under optimum experimental conditions, the CL intensity was linearly related to the concentration of levodopa from 3.4 x 10(-8) to 2.4 x 10(-5) mol/L and the detection limit was 1.1 x 10(-8) mol/L (s:n = 3). The relative standard deviation (RSD) of the proposed method calculated from 20 replicate injection of 3 x 10(-7) mol/L levodopa was 3.3%. The correlation coefficient was 0.997. The method was successfully applied to the determination of levodopa in commercial pharmaceutical formulations and spiked urine samples.
Alam
Seikh Mafiz
SM
Department of Chemistry, Kyungpook National University, Taegu 702-701, Republic of Korea.
Karim
Mohammad Mainul
MM
Lee
Sang Hak
SH
Wabaidur
Saikh Mohammad
SM
Chung
Hye Young
HY
Choi
Jong Ha
JH
Kang
Meea
M
D017022:Flow Injection Analysis / Q000379:methods; D007980:Levodopa / Q000032:analysis* / Q000652:urine; D008163:Luminescent Measurements / Q000379:methods*; D008722:Methods; D004364:Pharmaceutical Preparations / Q000032:analysis; D011196:Potassium Permanganate; D013447:Sulfites
D004364:Pharmaceutical Preparations; D013447:Sulfites; D011196:Potassium Permanganate; D007980:Levodopa