title: Immunogold for detection of avian leukosis/sarcoma virus in formalin-fixed heart and kidney.
abstract: Immunoprobes are used in the qualitative and semi-quantitative demonstration of antigens in clinical samples. In the present study, an immunogold method has been used for routine and rapid detection of avian leukosis/sarcoma virus (AL/SV) in sections of formalin-fixed chicken heart and kidney. Application of this ultrastructural indirect immunogold labelling technique (IILT) will enable pathologists to obtain a definitive diagnosis of avian AL/SV infection when inclusions or putative viral particles are observed by light or electron microscopy. IILT also may be used in research on AL/SV infections.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Medicine, The University of Georgia, Athens, Georgia, USA.
authors.forename: W L
authors.initials: WL
authors.lastname: Steffens
authors.forename: M A
authors.initials: MA
authors.lastname: Goodwin
authors.forename: M B
authors.initials: MB
authors.lastname: Ard