Alteration of GyrA amino acid required for ciprofloxacin resistance in Klebsiella pneumoniae isolates in China.
Resistance to ciprofloxacin was detected in 111 (48.1%) isolates of Klebsiella pneumoniae from China. GyrA alterations were identified in the ciprofloxacin-resistant and ciprofloxacin-susceptible isolates. The results, including previously published data, indicate that the single substitution Ser83-->Ile and three types of double mutations at Ser83 and Asp87 were required for ciprofloxacin resistance (P < 0.05).
Fu
Yingmei
Y
Department of Medical Microbiology, Harbin Medical University, Pathogenic Biology Key Laboratory of Heilongjiang Province, 194 Xue Fu Road, Harbin 150086, China.
Guo
Lishuang
L
Xu
Yan
Y
Zhang
Wenli
W
Gu
Jiaao
J
Xu
Jianfeng
J
Chen
Xiaobei
X
Zhao
Yuehui
Y
Ma
Jiayu
J
Liu
Xinghan
X
Zhang
Fengmin
F
D019943:Amino Acid Substitution; D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002939:Ciprofloxacin / Q000494:pharmacology*; D027081:DNA Gyrase / Q000235:genetics* / Q000378:metabolism; D024881:Drug Resistance, Bacterial / Q000235:genetics; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D015363:Quinolones / Q000494:pharmacology
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D015363:Quinolones; D002939:Ciprofloxacin; D027081:DNA Gyrase