title: Molecular dissection of the human B cell response against cytomegalovirus infection by lambda display.
abstract: Human cytomegalovirus (HCMV), a ubiquitous herpesvirus, is the main cause of congenital abnormalities and mental retardation in newborns and is also responsible for severe life-threatening complications in immunocompromised individuals, including AIDS patients and transplant recipients. The disorders generated by cytomegalovirus are closely associated with the competence of the host immune system and both humoral and cell-mediated mechanisms are involved in the response to viral infection. To identify viral proteins recognized by host antibody responses, a cytomegalovirus genome library was created and displayed on lambda bacteriophage. The challenge of such a library with sera from individuals with congenital or acquired infection allowed the identification of a wide panel of recombinant bacteriophages carrying cytomegalovirus B cell epitopes. Epitope-containing fragments within the families of tegument proteins (pUL25, pUL32), structural proteins (pUL48, pUL56) and glycoproteins (pUL55) were identified. Moreover, library screening permitted isolation of phage clones carrying an antigenic region of an uncharacterized HCMV protein encoded by the UL71 open reading frame (ORF), highlighting the potential of lambda display technology in antigen and epitope discovery.
authors.affiliation: Kenton Laboratories, Kenton S.r.l., Rome, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Beghetto
authors.forename: Francesca De
authors.initials: FD
authors.lastname: Paolis
authors.forename: Andrea
authors.initials: A
authors.lastname: Spadoni
authors.forename: Paola
authors.initials: P
authors.lastname: Del Porto
authors.forename: Wilma
authors.initials: W
authors.lastname: Buffolano
authors.forename: Nicola
authors.initials: N
authors.lastname: Gargano
mesh_terms: D000328:Adult; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D001402:B-Lymphocytes / Q000276:immunology*; D010582:Bacteriophage lambda / Q000235:genetics; D003587:Cytomegalovirus / Q000235:genetics / Q000276:immunology; D003586:Cytomegalovirus Infections / Q000151:congenital / Q000276:immunology* / Q000821:virology; D004797:Enzyme-Linked Immunosorbent Assay; D018985:Epitopes, B-Lymphocyte / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005260:Female; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D019151:Peptide Library; D011237:Predictive Value of Tests; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology* / Q000821:virology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology; D012680:Sensitivity and Specificity; D014764:Viral Proteins / Q000235:genetics* / Q000276:immunology / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D018985:Epitopes, B-Lymphocyte; D007074:Immunoglobulin G; D019151:Peptide Library; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D005982:Glutathione Transferase