Production of cellulolytic and hemicellulolytic enzymes from Aureobasidium pulluans on solid state fermentation.
This article investigates a strain of the yeast Aureobasidium pullulans for cellulase and hemicellulase production in solid state fermentation. Among the substrates analyzed, the wheat bran culture presented the highest enzymatic production (1.05 U/mL endoglucanase, 1.3 U/mL beta-glucosidase, and 5.0 U/mL xylanase). Avicelase activity was not detected. The optimum pH and temperature for xylanase, endoglucanase and beta-glucosidase were 5.0 and 50, 4.5 and 60, 4.0 and 75 degrees C, respectively. These enzymes remained stable between a wide range of pH. The beta-glucosidase was the most thermostable enzyme, remaining 100% active when incubated at 75 degrees C for 1 h.
Leite
Rodrigo Simões Ribeiro
RS
Laboratório De Bioquímica E Microbiologia Aplicada, IBILCE-Instituto De Biociências, Letras E Ciências Exatas, Universidade Estadual Paulista, Rua Cristóvão, Colombo, 2265, São José Do Rio Preto, 15054-000, São Paulo, CEP, Brazil.
Bocchini
Daniela Alonso
DA
Martins
Eduardo Da Silva
Eda S
Silva
Dêenis
D
Gomes
Eleni
E
Da Silva
Roberto
R
D001203:Ascomycota / Q000201:enzymology*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods; D002480:Cellulase / Q000378:metabolism*; D004043:Dietary Fiber / Q000382:microbiology*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D005285:Fermentation; D006026:Glycoside Hydrolases / Q000378:metabolism*; D014908:Triticum / Q000382:microbiology*
D004043:Dietary Fiber; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases