Characterization of regulatory intronic and exonic sequences involved in alternative splicing of scavenger receptor class B gene.
Scavenger receptor class B type I (SR-BI) is a major receptor of the high-density lipoprotein that mediates cholesterol efflux and reverse cholesterol transport. Alternative splicing of the scavenger receptor class B (SR-B) gene is observed and different splice forms, SR-BI and scavenger receptor class B type II (SR-BII), have been shown to function and localize in distinct ways. We have previously shown that SR-B alternative splicing regulation is associated with splicing factor ASF/SF2. In this study, using a SR-B minigene as a model, we determined the critical regulatory regions in the upstream intron, intron 11, by serial deletion and mutation analyses. We also further characterized the regulatory elements in intron 11 as well as in the skipped exon, exon 12. Moreover, we studied the interactions of these elements with the splicing factor ASF/SF2. This study provides new insights into the mechanism of SR-B splicing and it is important for further study on the mechanism of SR-B alternative splicing regulation, such as its regulation by estrogen.
Zhang
Xiaohui
X
Department of Obstetrics & Gynecology, University of South Florida College of Medicine, 12901 Bruce B Downs Boulevard, MDC 37, Tampa, FL 33612, USA.
Merkler
Kathleen A
KA
McLean
Mark P
MP
D017398:Alternative Splicing; D001483:Base Sequence; D045744:Cell Line, Tumor; D005091:Exons; D006801:Humans; D007438:Introns; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000378:metabolism; D012322:RNA Precursors / Q000378:metabolism; D016601:RNA-Binding Proteins; D051122:Scavenger Receptors, Class B / Q000235:genetics*; D000068103:Serine-Arginine Splicing Factors
D009687:Nuclear Proteins; D012322:RNA Precursors; D016601:RNA-Binding Proteins; D051122:Scavenger Receptors, Class B; D000068103:Serine-Arginine Splicing Factors