Blood outgrowth endothelial cells from chronic myeloid leukaemia patients are BCR/ABL1 negative.
The existence of adult haemangioblasts with dual haematopoietic and endothelial developmental potential was confirmed after detection of Ph(+) vascular endothelial cells in chronic myeloid leukaemia (CML) patients. Blood outgrowth endothelial cells (OECs) from CML patients were found not to harbour the Philadelphia translocation and were thus not clonally related to BRC/ABL1(+) hematopoietic progenitors, but comprised a distinct subfraction of endothelial cells. Remarkably, the frequency of CML-derived OECs was 9-fold higher as compared to healthy donors (n = 19 and n = 300, respectively; P < 0.0001) and these cells showed increased proliferative potential, possibly reflecting the mobilisation of OEC progenitors by pro-angiogenic cytokines.
Otten
Jasmin
J
Department of Internal Medicine II, Cancer Centre, University Hospital Hamburg-Eppendorf, Hamburg, Germany.
Schultze
Alexander
A
Schafhausen
Philippe
P
Otterstetter
Svenja
S
Dierlamm
Judith
J
Bokemeyer
Carsten
C
Brummendorf
Tim H
TH
Fiedler
Walter
W
Loges
Sonja
S
D014408:Biomarkers, Tumor / Q000378:metabolism; D042783:Endothelial Cells / Q000473:pathology*; D016313:Genes, abl; D006801:Humans; D016130:Immunophenotyping; D017404:In Situ Hybridization, Fluorescence; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000473:pathology*; D009389:Neovascularization, Pathologic / Q000473:pathology; D016133:Polymerase Chain Reaction
D014408:Biomarkers, Tumor