Optical coherence Doppler tomography quantifies laser speckle contrast imaging for blood flow imaging in the rat cerebral cortex.
A dual-imaging modality is demonstrated for high-resolution quantitative imaging of local cerebral blood flow in the rat cortex by combining simultaneous spectral-domain Doppler optical coherence tomography (SDOCT) and full-field laser speckle contrast imaging (LSCI). Preliminary studies in tissue flow phantom and cocaine-induced cerebral blood flow changes indicated that by correlating coregistered cortical arterial blood flow, the relative measurement of flow changes by LSCI could be accurately calibrated by the absolute flow imaging provided by SDOCT (least square fit, r(2) approximately 0.96). Quantitative LSCI of cerebral blood flow is crucial to the quantitative analyses of the spatiotemporal hemodynamics of functional brain activations and thus improved understanding of neural process.
Luo
Zhongchi
Z
Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, New York 11794-8181, USA.
Wang
Zhenguo
Z
Yuan
Zhijia
Z
Du
Congwu
C
Pan
Yingtian
Y
D000818:Animals; D002540:Cerebral Cortex / Q000098:blood supply / Q000502:physiology*; D002560:Cerebrovascular Circulation / Q000502:physiology*; D017078:Laser-Doppler Flowmetry / Q000295:instrumentation / Q000379:methods; D007834:Lasers; D019047:Phantoms, Imaging; D051381:Rats; D012039:Regional Blood Flow / Q000502:physiology; D041623:Tomography, Optical Coherence / Q000379:methods