[An analysis on chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization].
OBJECTIVE
To analyze the numerical aberration of chromosome X, Y and 18 in the spermatozoa of asthenospermia patients by triple-color fluorescence in situ hybridization.


METHODS
The experiment included 10 asthenospermia patients and 5 healthy men with normal semen quality as controls. Fluorescence in situ hybridization (FISH) and probes for chromosomes including X, Y and 18 were used to determine the frequency of the aneuploid of the chromosomes in spermatozoa.


RESULTS
Of the 45,547 spermatozoa counted from the semen samples, the hybridization rate was 99.18%. The frequencies of the chromosome disomies including XX18, XY18, YY18, X1818 and Y1818 were (0.124 +/- -0.086)%, (0.360 +/- 0.380)%, (0.109 +/- 0.195)%, (0.342 +/- 0.746)% and (0.299 +/- 0.564)% in the case group and (0.014 +/- 0.019)%, (0.090 +/- 0.080)%, (0.030 +/- 0.031)%, (0.068 +/- 0.103)% and (0.075 +/- 0.083)% in the control. The sperm aneuploid rate was 9.25% in the former and 2.70% in the latter, with significant difference in between (P< 0.01).


CONCLUSIONS
Asthenospermia patients have a higher aneuploid rate of sperm chromosome than normal fertile men. However, larger samples are yet to be studied to obtain more scientific evidence.
Li
Lian-Bing
LB
Chongqing Institute of Science and Technology for National Population and Family Planning, Chongqing 400020, China.
Xia
Yan-Kai
YK
Li
Xin-Sheng
XS
Lü
Jing
J
Ma
Ming-Fu
MF
Song
Ling
L
Ji
Yuan
Y
Yang
Ji-Gao
JG
Zhang
Tian-Feng
TF
Chen
Xiao-Xing
XX
Rong
Ying
Y
Zhao
Le-Tian
LT
D000782:Aneuploidy; D053627:Asthenozoospermia / Q000235:genetics*; D020223:Chromosome Painting / Q000379:methods*; D002887:Chromosomes, Human, Pair 18; D041321:Chromosomes, Human, X; D041322:Chromosomes, Human, Y; D006801:Humans; D008297:Male; D012729:Sex Chromosome Aberrations; D013094:Spermatozoa / Q000378:metabolism