[Utilization of the stable ectopic expression cell line as a model for the investigation of RIG-G gene].
OBJECTIVE
To investigate the biological function of RIG-G gene by establishing a cell line stably expressing RIG-G protein.


METHODS
Ectopic RIG-G gene was transfected into U937 cells by using Tet-off expression system. Changes before and after RIG-G expression were detected for cell growth, cell morphology, cell surface antigen and cell cycle regulating proteins.


RESULTS
RIG-G protein arrested the cells at G0/G1 phase and inhibited cell growth by increasing the cell cycle inhibitors P21 and P27. As compared to that in control group, the proportion of cells at G0/G1 phase in RIG-G-expressing cell group increased from (43.9 +/- 5.6)% to (63.9 +/- 2.3)% (P < 0.01). The rate of growth inhibition was (68.7 +/- 0.2)%. In addition, an increase in CD11C expression [(61.3 +/- 1.1)% vs. (18.0 +/- 0.4)% (P < 0.01)] and in cells with morphologic features of partial differentiation (smaller cell size, reduced nucleus-cytoplasm ratio, notched nucleus and coarse chromatin) was also observed in RIG-G-expressing cells.


CONCLUSIONS
RIG-G has potential abilities to inhibit cell proliferation and promote cell differentiation.
Xiao
Shu
S
Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
Jia
Pei-min
PM
Song
Man-gen
MG
Li
Dong
D
Pan
Xiao-rong
XR
Chen
Zhu
Z
Tong
Jian-hua
JH
D002453:Cell Cycle / Q000235:genetics; D002454:Cell Differentiation; D049109:Cell Proliferation; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27 / Q000235:genetics / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000502:physiology; D010957:Plasmids / Q000235:genetics; D014162:Transfection; D020298:U937 Cells
D050759:Cyclin-Dependent Kinase Inhibitor p21; C116468:IFIT3 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D050760:Cyclin-Dependent Kinase Inhibitor p27