Comparison of 99mTc- and 18F-ubiquicidin autoradiography to anti-Staphylococcus aureus immunofluorescence in rat muscle abscesses.
99mTc-ubiquicidin (UBI) 29-41 is under clinical evaluation for discrimination between bacterial infection and unspecific inflammation. We compared the distribution of 99mTc-UBI 29-41, the potential PET tracers 18F-UBI 29-41 and 18F-UBI 28-41, and 3H-deoxyglucose (DG) in rat muscle abscesses to that of anti-Staphylococcus aureus immunofluorescent imaging.


METHODS
Calf abscesses were induced in 15 CDF-Fischer rats after inoculation of Staphylococcus aureus. One to 6 d later, either 18F-UBI 29-41 and 3H-DG (n = 5) or 18F-UBI 28-41 and 3H-DG (n = 6) or 99mTc-UBI 29-41 and 3H-DG (n = 4) were injected simultaneously. Dual-tracer autoradiography of the abscess area was compared with the distribution of bacteria and macrophages.


RESULTS
The UBI derivates exhibited increased uptake in the abscess area that partly matched 3H-DG uptake and macrophage infiltration but showed no congruity with areas that were highly positive for bacteria.


CONCLUSIONS
A specific binding of UBI derivatives to Staphylococcus aureus in vivo could not be confirmed in this study.
Salber
Dagmar
D
C. & O. Vogt Institute of Brain Research, University Hospital Düsseldorf, Düsseldorf, Germany.
Gunawan
Johannes
J
Langen
Karl-Josef
KJ
Fricke
Eva
E
Klauth
Peter
P
Burchert
Wolfgang
W
Zijlstra
Sijtse
S
D000038:Abscess / Q000000981:diagnostic imaging*; D000818:Animals; D001345:Autoradiography / Q000379:methods*; D005455:Fluorescent Antibody Technique; D005462:Fluorine Radioisotopes; D009220:Myositis / Q000000981:diagnostic imaging*; D011877:Radionuclide Imaging; D019275:Radiopharmaceuticals; D051381:Rats; D011916:Rats, Inbred F344; D015203:Reproducibility of Results; D012135:Respiratory Sounds; D012269:Ribosomal Proteins; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000000981:diagnostic imaging*; D013667:Technetium
D005462:Fluorine Radioisotopes; D019275:Radiopharmaceuticals; D012269:Ribosomal Proteins; C040469:ribosomal protein S30; D013667:Technetium