title: Up-regulation of JAM-1 in AR42J cells treated with activin A and betacellulin and the diabetic regenerating islets.
abstract: Pancreatic AR42J cells demonstrate the pluripotency in precursor cells of the gut endoderm and also provide an excellent model system to study the differentiation of the pancreas. Using the mRNA differential display technique, we identified junctional adhesion molecule-1 (JAM-1), a component of the tight junction, was highly up-regulated during the differentiation of AR42J cells, although junctions were not formed. The expression level of JAM-1 showed an up-regulation in the mRNA level after 3 hours and in the protein level after 24 hours in [activin A + betacellulin]-treated AR42J cells. The expressions of its signaling molecules, PAR-3 and atypical PKC lambda, also increased after the addition of activin A + betacellulin. When JAM-1 was over-expressed in [activin A + betacellulin]-treated AR42J cells, tagged-JAM-1 was observed in cytoplasm as vesicular structures and JAM-1 was colocalized with Rab3B and Rab13, members of the Rab family expressed at tight junctions. In streptozotocin-induced regenerating islets, the expression of JAM-1 was also up-regulated in the mRNA level and the protein level. JAM-1 might therefore play an important role in the differentiation of AR42J cells and the regeneration of pancreatic islets.
authors.affiliation: Department of Gastroenterology, Graduate School of Medicine, University of Tokyo, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Yoshikumi
authors.forename: Hideki
authors.initials: H
authors.lastname: Ohno
authors.forename: Junko
authors.initials: J
authors.lastname: Suzuki
authors.forename: Masashi
authors.initials: M
authors.lastname: Isshiki
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Morishita
authors.forename: Hirohide
authors.initials: H
authors.lastname: Ohnishi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yasuda
authors.forename: Masao
authors.initials: M
authors.lastname: Omata
authors.forename: Toshiro
authors.initials: T
authors.lastname: Fujita
authors.forename: Hirosato
authors.initials: H
authors.lastname: Mashima
mesh_terms: D028341:Activins / Q000494:pharmacology*; D000818:Animals; D066259:Betacellulin; D015815:Cell Adhesion Molecules / Q000096:biosynthesis*; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000503:physiopathology; D004285:Dogs; D006801:Humans; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D007515:Islets of Langerhans / Q000502:physiology; D008297:Male; D010179:Pancreas / Q000166:cytology*; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000378:metabolism*; D015854:Up-Regulation; D020692:rab3 GTP-Binding Proteins / Q000096:biosynthesis
chemical_list: C587851:BTC protein, human; D066259:Betacellulin; C587853:Btc protein, rat; D015815:Cell Adhesion Molecules; C520495:F11r protein, rat; D036341:Intercellular Signaling Peptides and Proteins; C422405:activin A; D028341:Activins; C492132:Rab3b protein, rat; D020692:rab3 GTP-Binding Proteins