title: [Optimized preparation of ginkgolides A and B long-circulating solid lipid nanoparticles by central composite design and response surface method].
abstract: OBJECTIVE
  To optimize the formulation of long-circulating solid lipid nanoparticles (LSLN) carrying ginkgolides A and B (GAB) extracted from traditional Chinese medicinal herbs.
  
  
  METHODS
  GAB-LSLN was prepared by high pressure homogenization. The effects of such factors as the contents of glycerol monostearate, soybean lecithin and Myrj 59 on the mean diameter, entrapment efficiency and drug loading were investigated using central composite design and response surface method. The data were analyzed using multi-linear equation and second-order polynomial equation for selecting the optimal conditions for LSLN preparation.
  
  
  RESULTS
  The method of second-order polynomial equation was superior to multi-linear equation method in view of the multiple correlation coefficients. Under the optimal conditions, the mean diameter, entrapment efficiency, and drug loading of the GAB-LSLN were 169.5 nm, 92.3% and 5.1%, respectively.
  
  
  CONCLUSIONS
  The optimized process for GAB-LSLN preparation is stable, feasible and allows high inclusion rate in the large-scale production of GAB-LSLN.
authors.affiliation: Department of Pharmacy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. lm983566@163.com
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.forename: Jian-hai
authors.initials: JH
authors.lastname: Chen
authors.forename: Fu-rong
authors.initials: FR
authors.lastname: Dong
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liu
mesh_terms: D003692:Delayed-Action Preparations / Q000138:chemical synthesis* / Q000737:chemistry; D004339:Drug Compounding / Q000379:methods; D046934:Ginkgolides / Q000737:chemistry*; D007783:Lactones / Q000737:chemistry*; D008081:Liposomes; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D003692:Delayed-Action Preparations; D046934:Ginkgolides; D007783:Lactones; D008081:Liposomes; C045856:ginkgolide B; C477042:ginkgolide A