Disruption of sscR encoding a gamma-butyrolactone autoregulator receptor in Streptomyces scabies NBRC 12914 affects production of secondary metabolites.
We report the cloning and sequence analysis of a gamma-butyrolactone autoregulator regulatory island that includes an sscR gene encoding the gamma-butyrolactone autoregulator receptor from Streptomyces scabies NBRC 12914, a plant pathogenic strain. gamma-Butyrolactone autoregulators trigger secondary metabolism, and sometimes morphological differentiation in the Gram-positive genus Streptomyces through binding to a specific autoregulator receptor. This gene cluster showed close similarity to other regulatory islands of Streptomyces origin that are responsible for the control of secondary metabolism. The recombinant SscR protein expressed in Escherichia coli prefers a gamma-butyrolactone autoregulator containing a long C-2 side chain and beta-hydroxyl group at the C-6 position. An inactivation experiment confirmed that this gamma-butyrolactone autoregulator receptor was involved in secondary metabolism but had no effects on the morphological differentiation. In the sscR-deleted mutant, the binding activity of the gamma-butyrolactone autoregulator was completely abolished, suggesting that its primary role is to detect the presence of an autoregulator in the environment. HPLC analysis of the culture broth showed that some peaks disappeared and new peaks that were not present in the broth of the wild-type strain appeared.
Kitani
S
S
International Center for Biotechnology, Osaka University, Suita, Osaka, 565-0871, Japan.
Hoshika
M
M
Nihira
T
T
D015107:4-Butyrolactone / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D005809:Genes, Regulator; D008969:Molecular Sequence Data; D011963:Receptors, GABA-A / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D013302:Streptomyces / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*
C037481:4-butyrolactone receptor; D001426:Bacterial Proteins; D011963:Receptors, GABA-A; D015107:4-Butyrolactone