title: Meso-dihydroguaiaretic acid isolated from Saururus chinensis inhibits cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells.
abstract: Meso-dihydroguaiaretic acid (MDGA) is a medicinal herbal product isolated from the aerial parts of Saururus chinensis that inhibits the cyclooxygenase-2 (COX-2)-dependent phase of prostaglandin D(2) (PGD(2)) generation in bone marrow-derived mast cells (BMMC) (IC(50) 9.8 microM). However, this compound did not inhibit COX-2 protein expression in BMMC at concentrations up to 30 microM, indicating that MDGA directly inhibits COX-2 activity. In addition, this compound consistently inhibited the production of leukotriene C(4) (IC(50) 1.3 microM). These results demonstrate that MDGA inhibits both COX-2 and 5-lipoxygenase. Furthermore, this compound strongly inhibited the degranulation reaction in BMMC (IC(50) 11.4 microM). Therefore, this compound might provide a basis for novel anti-inflammatory drug development.
authors.affiliation: College of Pharmacy, Yeungnam University, Gyongsan, 712-749, Korea.
authors.forename: Tae Chul
authors.initials: TC
authors.lastname: Moon
authors.forename: Chang Seob
authors.initials: CS
authors.lastname: Seo
authors.forename: Kyungmi
authors.initials: K
authors.lastname: Haa
authors.forename: Jin Cheul
authors.initials: JC
authors.lastname: Kim
authors.forename: Nam Kyung
authors.initials: NK
authors.lastname: Hwang
authors.forename: Tae Gyun
authors.initials: TG
authors.lastname: Hong
authors.forename: Jee Hyeun
authors.initials: JH
authors.lastname: Kim
authors.forename: Do Hun
authors.initials: DH
authors.lastname: Kim
authors.forename: Jong Keun
authors.initials: JK
authors.lastname: Son
authors.forename: Hyeun Wook
authors.initials: HW
authors.lastname: Chang
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000302:isolation & purification / Q000494:pharmacology*; D016718:Arachidonic Acid / Q000378:metabolism; D001854:Bone Marrow Cells / Q000187:drug effects* / Q000201:enzymology; D002478:Cells, Cultured; D052246:Cyclooxygenase 2 Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D016859:Lipoxygenase Inhibitors; D008297:Male; D008407:Mast Cells / Q000187:drug effects* / Q000201:enzymology; D051379:Mice; D008807:Mice, Inbred BALB C; D032134:Saururaceae
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D052246:Cyclooxygenase 2 Inhibitors; D017705:Lignans; D016859:Lipoxygenase Inhibitors; D016718:Arachidonic Acid; C015816:dihydroguaiaretic acid; D006139:Guaiacol