[Construction of the recombinant adenovirus vector bearing dual-survivin shRNA].
OBJECTIVE
To construct a recombinant adenovirus vector bearing dual-survivin short hairpin RNA (shRNA).


METHODS
Dual-survivin shRNA was designed and synthesized respectively, both inserted into adenovirus DNA. The recombinant adenovirus vector was confirmed via both sequencing and restriction digestion analysis, and then linearized and transfected into the HEK 293 cell line to generate recombinant adenoviruses.


RESULTS
The recombinant adenovirus vector was constructed and the target sequence was obtained.


CONCLUSIONS
The construction of the recombinant adenovirus vector provides a basis for the research of potential gene therapy for prostate cancer.
Gao
Peng
P
Department of Urology, Second Hospital Affiliated to Soochow University, Suzhou, Jiangsu 215004, China. ag2525@tom.com
Shan
Yu-Xi
YX
Yang
Dong-Rong
DR
D000256:Adenoviridae / Q000235:genetics*; D002460:Cell Line; D004262:DNA Restriction Enzymes / Q000378:metabolism; D005822:Genetic Vectors / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics*; D016133:Polymerase Chain Reaction; D034741:RNA, Small Interfering / Q000235:genetics*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D000077022:Survivin; D014162:Transfection
C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D008869:Microtubule-Associated Proteins; D009363:Neoplasm Proteins; D034741:RNA, Small Interfering; D011993:Recombinant Fusion Proteins; D000077022:Survivin; D049452:Green Fluorescent Proteins; D004262:DNA Restriction Enzymes