title: Tumor stroma marker endosialin (Tem1) is a binding partner of metastasis-related protein Mac-2 BP/90K.
abstract: Tumor development involves complex bidirectional interactions between tumor cells and host stromal cells. Endosialin (Tem1) has been identified as a highly O-glycosylated transmembrane glycoprotein, which is specifically expressed by tumor vessel-associated pericytes and stromal fibroblasts of a wide range of human tumors. Recent experiments in endosialin-deficient mice have unraveled a critical role of endosialin in site-specific tumor progression and metastasis. To molecularly understand the mechanisms of endosialin function, we aimed to identify extracellular endosialin ligands and identified Mac-2 BP/90K as a specific interaction partner. Detailed biochemical analyses identified a C-terminal fragment of Mac-2 BP/90K, which was shown to contain binding sites for galectin-3, and collagens as the structures responsible for endosialin binding. Subsequent expression analysis of Mac-2 BP/90K in vivo revealed weak or no expression in most normal tissues and strong up-regulation in tumor cells of human neoplastic tissues. Intriguingly, the expression patterns of Mac-2 BP/90K and endosialin were mutually exclusive in all human tissues. Correspondingly, loss-of-function adhesion experiments of Mac-2 BP/90K-expressing tumor cells on endosialin-expressing fibroblasts revealed a repulsive outcome of the Mac-2 BP/90K interaction. Taken together, the experiments identify a novel repulsive interaction between endosialin on stromal fibroblasts and Mac-2 BP/90K on tumor cells.
authors.affiliation: Joint Research Division Vascular Biology of the Medical Faculty Mannheim, University of Heidelberg and the German Cancer Research Center, Heidelberg, Germany.
authors.forename: Renate
authors.initials: R
authors.lastname: Becker
authors.forename: Martin C
authors.initials: MC
authors.lastname: Lenter
authors.forename: Tobias
authors.initials: T
authors.lastname: Vollkommer
authors.forename: Anja M
authors.initials: AM
authors.lastname: Boos
authors.forename: Dennis
authors.initials: D
authors.lastname: Pfaff
authors.forename: Hellmut G
authors.initials: HG
authors.lastname: Augustin
authors.forename: Sven
authors.initials: S
authors.lastname: Christian
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D000951:Antigens, Neoplasm / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D008297:Male; D008562:Membrane Glycoproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000235:genetics; D014018:Tissue Distribution; D015854:Up-Regulation
chemical_list: D015703:Antigens, CD; D000951:Antigens, Neoplasm; C078203:CD248 protein, human; D008024:Ligands; D008562:Membrane Glycoproteins; D010446:Peptide Fragments; D034741:RNA, Small Interfering; C114782:TAA90K protein, human