Functionalized alginate as immobilization matrix in enantioselective L (+) lactic acid production by Lactobacillus delbrucekii.
The imperative role of functionalized natural alginate in immobilization of Lactobacillus delbrucekii (NCIM 2365) cells in production of optically pure L (+) lactic acid was studied. L. delbrucekii cells were immobilized in alginate, succinylated alginate and carrageenan to evaluate the bead stability and selectivity towards production of optically pure L (+) lactic acid. The scanning electron microscopic studies of free and immobilized cells show little morphological changes. The present study highlights the use of functionalized alginate-immobilized L. delbrucekii cells in production of L (+) lactic acid in higher yields (0.93 Yp/s in grams) with an improved enantioselectivity (99%). In addition, they further revealed decreased by-product formation (acetic and propionic acid) when compared to free and other immobilized cell fermentation.
Rao
Ch Subba
ChS
Indian Institute of Chemical Technology, Hyderabad 500 007, India.
Prakasham
R S
RS
Rao
A Bhaskar
AB
Yadav
J S
JS
D000464:Alginates / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005285:Fermentation; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000378:metabolism*; D008855:Microscopy, Electron, Scanning; D017550:Spectroscopy, Fourier Transform Infrared; D013237:Stereoisomerism
D000464:Alginates; D006603:Hexuronic Acids; D019344:Lactic Acid; D020723:Glucuronic Acid