[Interaction of taxifolin (dihydroquercetin) with dimyristoylphosphatidylcholine multilamellar liposomes].
Differential scanning calorimetry was used to study the influence of the flavonoid taxifolin (dihydroquercetin) on the temperature-dependent phase transition of dimyristoylphosphatidylcholine multilamellar liposomes. Taxifolin was added to organic solution of the lipid during the procedure of liposomes preparation (addition from-within) or to a suspension of prepared liposomes (addition from-without). In the first case, liposomes contained from 2 to 50 mol% of taxifolin added from-within; in the second case, lyposomes were treated with 0.001% or 0.01% taxifolin. In both cases, the effect was similar. When the concentration of taxifolin increased, the temperature of lipid melting decreased while the width of transition considerably enlarged. Freeze-fracture electron microscopy revealed that taxifolin did not rupture multilamellar liposomes, while the formation of ripple-phase was retarded in all bilayers even when the liposomes were treated from without. This suggested the ability of taxifolin to penetrate through numerous bilayers of multilamellar liposomes.
Tarakhovskiĭ
Iu S
IuS
Kuznetsova
S M
SM
Vasil'eva
N A
NA
Egorochkin
M A
MA
Kim
Iu A
IuA
D002152:Calorimetry, Differential Scanning; D004134:Dimyristoylphosphatidylcholine / Q000737:chemistry*; D005614:Freeze Fracturing / Q000379:methods; D008081:Liposomes / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D044367:Phase Transition; D011794:Quercetin / Q000031:analogs & derivatives* / Q000737:chemistry
D008081:Liposomes; D011794:Quercetin; C003377:taxifolin; D004134:Dimyristoylphosphatidylcholine