title: Formation of guanosine(5')tetraphospho(5')adenosine cap structure by an unconventional mRNA capping enzyme of vesicular stomatitis virus.
abstract: The RNA-dependent RNA polymerase L protein of vesicular stomatitis virus (VSV) elicits GTPase and RNA:GDP polyribonucleotidyltransferase (PRNTase) activities to produce a 5'-cap core structure, guanosine(5')triphospho(5')adenosine (GpppA), on viral mRNAs. Here, we report that the L protein produces an unusual cap structure, guanosine(5')tetraphospho(5')adenosine (GppppA), that is formed by the transfer of the 5'-monophosphorylated viral mRNA start sequence to GTP by the PRNTase activity before the removal of the gamma-phosphate from GTP by GTPase. Interestingly, GppppA-capped and polyadenylated full-length mRNAs were also found to be synthesized by an in vitro transcription system with the native VSV RNP.
authors.affiliation: Department of Molecular Genetics, Section of Virology, Lerner Research Institute, Cleveland Clinic, 9500 Euclid Avenue, Cleveland, OH 44195, USA.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Ogino
authors.forename: Amiya K
authors.initials: AK
authors.lastname: Banerjee
mesh_terms: D015226:Dinucleoside Phosphates / Q000378:metabolism; D009713:Nucleotidyltransferases / Q000378:metabolism*; D012315:RNA Caps / Q000737:chemistry* / Q000378:metabolism*; D012367:RNA, Viral / Q000378:metabolism; D012324:RNA-Dependent RNA Polymerase / Q000378:metabolism*; D018116:Vesiculovirus / Q000201:enzymology*; D014764:Viral Proteins / Q000378:metabolism*; D000098556:mRNA Guanylyltransferases
chemical_list: D015226:Dinucleoside Phosphates; D012315:RNA Caps; D012367:RNA, Viral; D014764:Viral Proteins; C017354:guanosine 5'-triphosphate-5'-adenosine; D009713:Nucleotidyltransferases; C040530:L protein, vesicular stomatitis virus; D012324:RNA-Dependent RNA Polymerase; D000098556:mRNA Guanylyltransferases