title: Cytokine release and expression induced by OmpA proteins from the Gram-negative anaerobes, Porphyromonas asaccharolytica and Bacteroides fragilis.
abstract: OmpA proteins from Gram-negative anaerobes Porphyromonas asaccharolytica and Bacteroides fragilis induced release and expression of IL-1alpha, tumor necrosis factor (TNF)-alpha, IFN-gamma, IL-6, and IL-10 from murine splenocytes in vitro in a dose-dependent fashion. The release of the cytokines induced by B. fragilis Bf-OmpA was at much lower levels compared with P. asaccharolytica Omp-PA; Bf-OmpA did not induce release of IL-10. Omp-PA and Bf-OmpA were able to upregulate mRNA expression of the tested cytokines. The results obtained with refolded Bf-OmpA were similar to those with native Bf-OmpA. The data presented in this research demonstrate for the first time that Omps from anaerobic bacteria can induce the release of cytokines, suggesting that Omp-PA and Bf-OmpA may play important roles in the pathogenic processes of these bacteria.
authors.affiliation: The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
authors.forename: Lana
authors.initials: L
authors.lastname: Magalashvili
authors.forename: Shirley
authors.initials: S
authors.lastname: Lazarovich
authors.forename: Izabella
authors.initials: I
authors.lastname: Pechatnikov
authors.forename: Hannah M
authors.initials: HM
authors.lastname: Wexler
authors.forename: Yeshayahu
authors.initials: Y
authors.lastname: Nitzan
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001441:Bacteroides fragilis / Q000276:immunology*; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000235:genetics; D004306:Dose-Response Relationship, Immunologic; D020869:Gene Expression Profiling; D007963:Leukocytes, Mononuclear / Q000276:immunology; D008297:Male; D051379:Mice; D017861:Porphyromonas / Q000276:immunology*; D012333:RNA, Messenger / Q000096:biosynthesis; D013154:Spleen / Q000276:immunology; D015854:Up-Regulation
chemical_list: D001425:Bacterial Outer Membrane Proteins; D016207:Cytokines; D012333:RNA, Messenger; C078167:OMPA outer membrane proteins