[Isolation of a methane-utilizing bacterium and its application in measuring methane gas].
A bacterial strain ME16 was isolated from Jinyang Lake in Taiyuan of Shanxi Province, China. Gas chromatography analysis showed that the strain could use methane as the sole carbon and energy source. Based on 16S rDNA sequence analysis, the strain was identified as Pseudomonas aeruginosa. Effects of inoculum size, temperature, methane content and initial pH of media on cell growth were studied. In addition, we examined the response time of methane gas to dissolved oxygen and the relationship between consumption of dissolved oxygen and different methane gas content with PVA-H3BO3 immobilized cell of ME16 using electrochemical method. The optimal conditions for cell growth were 2% inoculum size, 25% methane content, 30 degrees C and pH 6.0. Response time was within 100 s after adding of immobilized cells to reaction system. The linear range of measured methane content was from 0 to 16%, with the correlation coefficient 0.9954. Hence, this strain has potential application in developing of methane biosensor.
Zhao
Gengui
G
Institute of Biotechnology, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China. chungui@sxu.edu.cn
Zheng
Jun
J
Wen
Guangming
G
Yang
Suping
S
Dong
Chuan
C
D049109:Cell Proliferation / Q000187:drug effects; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D005740:Gases / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008697:Methane / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010100:Oxygen / Q000737:chemistry / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000166:cytology / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D012995:Solubility; D013696:Temperature
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005740:Gases; D008697:Methane; D010100:Oxygen