Identification of LDL-receptor transcription stimulating peptides from soybean hydrolysate in human hepatocytes.
Soybean protein and its hydrolysate have been reported to have cholesterol-lowering property, but the responsible components are still largely unknown. In previous study, we found that soybean protein hydrolysate (SPH) prepared with the protease from Bacillus amyloliquefaciens FSE-68, strongly stimulates transcription of low density lipoprotein receptor (LDL-R). To identify LDL-R transcription stimulating peptides in human hepatocytes, the SPH was fractionated with gel permeation chromatograpy and the active fraction was further separated by using reverse-phase chromatography. Several peptides in the most active fraction were identified by LC/MS and MS/MS analysis. LDL-R transcription stimulating peptides were synthesized on the basis of identified sequences, and their effect on LDL-R transcription was tested in vitro. Among the synthesized peptides, Phe-Val-Val-Asn-Ala-Thr-Ser-Asn (FVVNATSN) showed the strongest activity, and LDL-R transcription of hepatic cells was increased to 248.8% (compared to 100% of untreated control) by FVVNATSN at a concentration of 100 microM. This study provides direct evidence that peptides derived from soybean protein can influence LDL-R transcription in hepatocytes.
Cho
Seong-Jun
SJ
Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.
Juillerat
Marcel A
MA
Lee
Cherl-Ho
CH
D001407:Bacillus / Q000201:enzymology; D002460:Cell Line; D022781:Hepatocytes / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D010447:Peptide Hydrolases / Q000378:metabolism; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000494:pharmacology*; D011973:Receptors, LDL / Q000235:genetics*; D030262:Soybean Proteins / Q000737:chemistry* / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
D010455:Peptides; D011973:Receptors, LDL; D030262:Soybean Proteins; D010447:Peptide Hydrolases