Capillary electrophoresis-mass spectrometry - a fast and reliable tool for the monitoring of milk adulteration.
The development of a rapid, simple and accurate analytical method aimed at the detection and quantification of bovine milk in either ovine or caprine milk samples by means of CE-MS analyses of whey proteins with high-ionic strength and presence of acidic running buffer is described. The high-ionic strength buffer was used in order to minimize the problems with the adsorption of the proteins onto the fused-silica capillary wall. The acidic running electrolyte, pH 1.9, was used to support the production of positive ions in electrospray. Highly linear dependences of the ratio of the sum of non-bovine beta-lactoglobulins (ovine or caprine) to the total beta-lactoglobulins in milk mixture (bovine plus ovine or bovine plus caprine) vs. the volume percentage of added bovine milk in ovine (or caprine) milk were obtained. This technique allowed the fast and reliable evaluation of milk adulteration. The amount of bovine milk added into the "non-bovine" ones can be well within the concentration range of 5-95%.
Muller
Lukás
L
Department of Analytical Chemistry, Faculty of Science, Palacky University, Olomouc, Czech Republic. mlluk@post.cz
Barták
Petr
P
Bednár
Petr
P
Frysová
Iveta
I
Sevcík
Juraj
J
Lemr
Karel
K
D000327:Adsorption; D000818:Animals; D002417:Cattle; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D005506:Food Contamination / Q000032:analysis*; D006041:Goats; D007782:Lactoglobulins / Q000032:analysis; D008892:Milk / Q000737:chemistry* / Q000191:economics; D008894:Milk Proteins / Q000032:analysis; D015203:Reproducibility of Results; D012756:Sheep; D012822:Silicon Dioxide; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods* / Q000706:statistics & numerical data; D000067816:Whey Proteins
D007782:Lactoglobulins; D008894:Milk Proteins; D000067816:Whey Proteins; D012822:Silicon Dioxide