Birth of normal offspring from mouse eggs activated by a phospholipase Czeta protein lacking three EF-hand domains.
Sperm-specific phospholipase C, PLCzeta, is a candidate for the Ca(2+) oscillation-inducing factor that is introduced into the ooplasm upon sperm-egg fusion. In addition to the 647-residue full-length PLCzeta, s-PLCzeta lacking the N-terminal 110 amino acids is known to be present in the mouse testis. In this study, we attempted to obtain full-term offspring from s-PLCzeta-activated eggs by round spermatid injection. Metaphase II-arrested eggs injected with a high RNA concentration of s-PLCzeta RNA normally developed to blastocysts. When the round spermatid nucleus was injected into telophase II-stage eggs previously activated by s-PLCzeta RNA, three live offspring were successfully obtained by transfer of the developed 4-cell embryos to pseudopregnant mice. These three offspring all grew to be normal adults and reproduced healthy second-generation mice.
Nakanishi
Tomoko
T
Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Japan. tomo@med.tottori-u.ac.jp
Ishibashi
Naoko
N
Kubota
Haruka
H
Inoue
Kimiko
K
Ogonuki
Narumi
N
Ogura
Atsuo
A
Kashiwabara
Shin-ichi
S
Baba
Tadashi
T
D000818:Animals; D000831:Animals, Newborn; D020832:EF Hand Motifs / Q000502:physiology*; D046149:Embryo Culture Techniques; D004622:Embryo, Mammalian; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D007527:Isoenzymes / Q000737:chemistry / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D009865:Oocytes / Q000187:drug effects*; D009866:Oogenesis / Q000187:drug effects; D010312:Parthenogenesis / Q000187:drug effects; D036801:Parturition / Q000187:drug effects*; D054801:Phosphoinositide Phospholipase C / Q000737:chemistry / Q000494:pharmacology*; D011247:Pregnancy; D013692:Telophase / Q000187:drug effects; D010738:Type C Phospholipases / Q000737:chemistry / Q000494:pharmacology*
D007527:Isoenzymes; D010738:Type C Phospholipases; D054801:Phosphoinositide Phospholipase C; C499234:Plcz1 protein, mouse