title: Evaluation of a point-of-care hematology analyzer for use in dogs and cats receiving chemotherapeutic treatment.
abstract: OBJECTIVE
  To compare WBC, neutrophil, and platelet counts and Hct values obtained with a point-of-care hematology analyzer with values obtained by a reference method for dogs and cats receiving chemotherapy.
  
  
  METHODS
  Cross-sectional study.
  
  
  METHODS
  105 dogs and 25 cats undergoing chemotherapy.
  
  
  METHODS
  Blood samples were analyzed with a point-of-care hematology analyzer and with an impedance- and laser-based analyzer with manual differential WBC counts. Results for WBC, neutrophil, and platelet counts and Hct were compared. Sensitivity and specificity of the point-of-care analyzer to detect leukopenia, neutropenia, and anemia were calculated.
  
  
  RESULTS
  554 canine and 96 feline blood samples were evaluated. Correlation coefficients for dogs and cats, respectively, were 0.92 and 0.95 for total WBC count, 0.91 and 0.88 for neutrophil count, 0.95 and 0.92 for Hct, and 0.93 and 0.71 for platelet count. Sensitivity and specificity, respectively, of the point-of-care analyzer to detect leukopenia were 100% and 75% for dogs and 100% and 68% for cats; to detect neutropenia were 80% and 97% for dogs and 100% and 80% for cats; to detect anemia were 100% and 80% for dogs and 100% and 66% for cats; and to detect thrombocytopenia were 86% and 95% for dogs and 50% and 87% for cats.
  
  
  CONCLUSIONS
  The point-of-care analyzer was reliable for monitoring CBCs of dogs and cats receiving chemotherapy. It had good to excellent correlation for WBC and neutrophil counts and Hct and accurately detected leukopenia, neutropenia, and anemia. Sensitivity of the analyzer for detecting thrombocytopenia was lower but acceptable.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH 43210, USA.
authors.forename: Ana
authors.initials: A
authors.lastname: Lara-Garcia
authors.forename: Kenji
authors.initials: K
authors.lastname: Hosoya
authors.forename: Cristina
authors.initials: C
authors.lastname: Iazbik
authors.forename: Nicole
authors.initials: N
authors.lastname: Westendorf
authors.forename: Stacey
authors.initials: S
authors.lastname: Gallant
authors.forename: Guillermo
authors.initials: G
authors.lastname: Couto
mesh_terms: D000740:Anemia / Q000097:blood / Q000175:diagnosis / Q000662:veterinary; D000818:Animals; D002371:Cat Diseases / Q000097:blood* / Q000175:diagnosis; D002415:Cats; D003430:Cross-Sectional Studies; D004283:Dog Diseases / Q000097:blood* / Q000175:diagnosis; D004285:Dogs; D004358:Drug Therapy / Q000662:veterinary*; D064420:Drug-Related Side Effects and Adverse Reactions; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D006403:Hematologic Tests / Q000295:instrumentation / Q000662:veterinary*; D007958:Leukocyte Count / Q000662:veterinary; D007970:Leukopenia / Q000097:blood / Q000175:diagnosis / Q000662:veterinary; D008297:Male; D009503:Neutropenia / Q000097:blood / Q000175:diagnosis / Q000662:veterinary; D009504:Neutrophils; D010976:Platelet Count / Q000662:veterinary; D019095:Point-of-Care Systems; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014730:Veterinary Medicine / Q000295:instrumentation*