title: Expression of zebrafish nos2b surrounds oral cavity.
abstract: Inducible nitric oxide synthase (NOS2) catalyzes the production of nitric oxide (NO), and is one of the factors establishing innate immunity. In zebrafish, Nos2 is represented by nos2a and nos2b. Here, we report the cloning and expression pattern of the zebrafish nos2b gene, which does not seem to participate in induced immune response. nos2b was mapped to zebrafish linkage group 15. The spatial and temporal expression pattern of nos2b in embryonic zebrafish was analyzed by whole-mount in situ hybridization. nos2b is expressed constitutively in two primordia located along the ventral midline. The first group of cells contributes to the neurohypophysis. Initially at the level of the ventral hindbrain, the second group of cells migrates closely with the thyroid primordium to its final position at the basihyal by 3 dpf. Thus, the analysis of expression pattern of nos2b reveals complex morphogenetic movements resulting in its expression surrounding the oral cavity.
authors.affiliation: Cancer and Developmental Cell Biology Division, Institute of Molecular and Cell Biology, National University of Singapore, Singapore.
authors.forename: Kar-Lai
authors.initials: KL
authors.lastname: Poon
authors.forename: Michael
authors.initials: M
authors.lastname: Richardson
authors.forename: Vladimir
authors.initials: V
authors.lastname: Korzh
mesh_terms: D000818:Animals; D001921:Brain / Q000196:embryology; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008954:Models, Biological; D008957:Models, Genetic; D009055:Mouth / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis* / Q000502:physiology; D013961:Thyroid Gland / Q000196:embryology; D013997:Time Factors; D014059:Tongue / Q000196:embryology; D015027:Zebrafish; D029961:Zebrafish Proteins / Q000096:biosynthesis* / Q000502:physiology
chemical_list: D029961:Zebrafish Proteins; D009569:Nitric Oxide; D019001:Nitric Oxide Synthase; D052247:Nitric Oxide Synthase Type II