[Molecular cloning and preliminary function study of a novel human gene AC3-33 related to suppress AP-1 activity].
Activator protein-1(AP-1) is an important transcription factor, and dysregulation of its activity has been associated with many human diseases, including various cancers. A novel human gene AC3-33 (GenBank name: c30rf33, Accession No. FLJ31139), which can suppress PMA and Ionomycin induced activation of AP-1, was identified from 650 human func-tion-known genes by using the high throughput-high content cell-based screening technology. The gene whose full cDNA length is 1391 bp containing 6 exons and 5 introns is located in the human chromosome 3q25.31. The predicted protein encoded by this gene contains 251 amino acids with a theoretical molecular weight of 29 kDa. Expression of the AC3-33 gene is widly found in adrenal glands and cervix. The amino acid sequences of AC3-33 is highly conserved, and has no homology to other known proteins. Subcellular localization studies show that the AC3-33 protein was localized in the cytoplasm. Our preliminary results showed that AC3-33 is an important novel gene related to supress AP-1 activity.
Liu
Peng
P
Department of Biology, Northchina Coal Medical College, Tangshan 063000, China. panpan424@163.com
Deng
Wei-Wei
WW
Gao
Peng
P
Lu
Yang
Y
Sun
Bo
B
Li
Ming
M
Zhao
Jie
J
Shi
Tai-Ping
TP
Zhang
Xiu-Jun
XJ
D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007438:Introns / Q000235:genetics; D015759:Ionomycin / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D011109:Polymethacrylic Acids / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism* / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D018808:Transcription Factor AP-1 / Q000235:genetics*
D011109:Polymethacrylic Acids; D011506:Proteins; D018808:Transcription Factor AP-1; D015759:Ionomycin