Proteomic analysis of MCF-7 cells treated with benzo[a]pyrene, dibenzo[a,l]pyrene, coal tar extract, and diesel exhaust extract.
Polycyclic aromatic hydrocarbon (PAH) DNA adducts have been associated with carcinogenesis, which is accompanied by multiple alterations in gene expression. We used two-dimensional electrophoresis to distinguish protein expression changes induced in MCF-7 cells by individual PAH (B[a]P and DB[a,l]P) and PAH mixtures (coal tar extract [SRM 1597] and diesel exhaust extract [SRM 1975]). Spots of interest were identified by MALDI-TOF-TOF. Our results have shown alterations in the expression of heat-shock proteins, cytoskeletal proteins, DNA associated proteins, and glycolytic and mitochondrial proteins. The proteins that were universally altered in expression were actin cytoplasmic 1, tubulin alpha and myosin light chain alkali, cyclophilin B, and heterogeneous ribonucleoprotein B1 (a protein involved in access to telomerase and mRNA maturation). Additional proteins with altered expression include histone H2A.1, heat-shock protein 70-2, galectin-3, nucleoside diphosphate kinase, ATP synthase, and electron transfer flavoprotein. While sharing similarities, each PAH treatment exhibited a unique proteomic fingerprint.
Hooven
Louisa Ada
LA
Department of Zoology, Oregon State University, Corvallis, OR, USA. hoovenl@onid.orst.edu <hoovenl@onid.orst.edu>
Baird
William M
WM
D001564:Benzo(a)pyrene / Q000633:toxicity*; D001580:Benzopyrenes / Q000633:toxicity*; D001943:Breast Neoplasms / Q000188:drug therapy / Q000378:metabolism*; D002273:Carcinogens / Q000633:toxicity*; D045744:Cell Line, Tumor; D003033:Coal Tar / Q000633:toxicity*; D004591:Electrophoresis, Polyacrylamide Gel; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D009363:Neoplasm Proteins / Q000737:chemistry / Q000378:metabolism; D020543:Proteome / Q000737:chemistry*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001335:Vehicle Emissions / Q000633:toxicity*
D001580:Benzopyrenes; D002273:Carcinogens; D009363:Neoplasm Proteins; D020543:Proteome; D001335:Vehicle Emissions; D001564:Benzo(a)pyrene; D003033:Coal Tar; C041517:dibenzo(a,l)pyrene