Determination of the antioxidative capacity of an antioxidant complex and idebenone: an in vitro rapid and sensitive method.
OBJECTIVE
The goal of this study was to determine the ability of two putative antioxidant solutions to suppress the formation of superoxide anion radicals created by optical excitation of a photosensitizer and measured by highly sensitive luminometric detection.


RESULTS
Solutions containing 3% antioxidant complex and 1% idebenone were tested. The antioxidant complex is an aqueous combination of botanical extracts rich in ferulic acid, caffeic acid, and other polyphenols. Idebenone is a lower molecular weight analog of coenzyme Q(10) (a potent antioxidant). Each was dissolved in lipid soluble reagents (solutions) and run through a Photochem (photochemiluminometer system) device. Their antioxidant capacity was expressed as nmol equivalents of synthetic vitamin E. Nine aliquots of each compound were measured. The mean antioxidant capacities for the 3% antioxidant complex and 1% idebenone were 525 +/- 23 and 213 +/- 14 nmol, respectively, with a statistically significant difference between the two compounds (P < 0.001).


CONCLUSIONS
The 3% antioxidant solution possesses a significant antioxidative capacity, which was 2.5 times greater than the known antioxidant idebenone in a 1% solution as shown by the quenching of superoxide anion radicals measured by photochemiluminescence. This method provides rapid, accurate, and sensitive measurement of the antioxidant properties of lipid-soluble compounds.
Zhai
Hongbo
H
Department of Dermatology, University of California, San Francisco, San Francisco, CA 94143-0989, USA. zhaih@derm.ucsf.edu
Cordoba-Diaz
Manuel
M
Wa
Chrystal
C
Hui
Xiaoying
X
Maibach
Howard I
HI
D000975:Antioxidants / Q000737:chemistry*; D002109:Caffeic Acids / Q000737:chemistry; D003373:Coumaric Acids / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry; D049449:Luminescence; D008163:Luminescent Measurements; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D059808:Polyphenols; D012995:Solubility; D013481:Superoxides / Q000032:analysis / Q000378:metabolism*; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry; D014466:Ultraviolet Rays; D014810:Vitamin E / Q000737:chemistry
D000975:Antioxidants; D002109:Caffeic Acids; D003373:Coumaric Acids; D005419:Flavonoids; D010636:Phenols; D010936:Plant Extracts; D059808:Polyphenols; D013481:Superoxides; D014451:Ubiquinone; D014810:Vitamin E; C004999:ferulic acid; C036619:idebenone; C040048:caffeic acid