Differential expression of three ABA-insensitive five binding protein (AFP)-like genes in wheat.
Abscisic acid (ABA) signaling includes positive and negative regulators in the signaling pathway. ABA-insensitive five (ABI5) binding protein (AtAFP), one of the negative regulators found in Arabidopsis, is involved in the proteolysis of a positive regulator, ABI5 (bZIP-type transcription factor). Three wheat orthologs (TaAFPs) of AtAFP were isolated. TaAFPs have a nuclear localization domain in the middle of the deduced amino acid sequence and an ABI5 binding domain in the C-terminal region as AtAFP. Three TaAFPs were located on the short arms of chromosomes 2A, 2B, and 2D of wheat, and based on their chromosomal locations, they were named TaAFP-A, TaAFP-B, and TaAFP-D. In comparison to AtAFP, which was activated in developing seeds and the early stage of germination, TaAFPs were expressed in a greater variety of tissues, such as flag leaves, roots, and leaves of seedlings, and developing grains. TaAFP-B was expressed predominantly in all tissues examined; TaAFP-A and TaAFP-D responded to ABA and stresses, such as salt and dehydration. These three TaAFPs may differentiate their roles in ABA signaling during wheat evolution.
Ohnishi
Naruhito
N
Research Institute for Bioresources, Okayama University, Kurashiki, Okayama, Japan.
Himi
Eiko
E
Yamasaki
Yoshiki
Y
Noda
Kazuhiko
K
D000040:Abscisic Acid / Q000502:physiology*; D000595:Amino Acid Sequence; D001483:Base Sequence; D001665:Binding Sites; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D018628:Gene Dosage; D015870:Gene Expression; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D020218:Response Elements; D017386:Sequence Homology, Amino Acid; D014157:Transcription Factors / Q000378:metabolism; D014908:Triticum / Q000235:genetics*
D002352:Carrier Proteins; D010940:Plant Proteins; D014157:Transcription Factors; D000040:Abscisic Acid