title: Human bocavirus infection in children hospitalized with acute gastroenteritis in China.
abstract: BACKGROUND
  Human bocavirus (HBoV) was first identified in children with acute respiratory-tract infections, but recent studies have revealed that HBoV is also frequently detected in fecal specimens from children with gastroenteritis.
  
  
  OBJECTIVE
  To investigate the prevalence of HBoV in children hospitalized with gastroenteritis in different areas of China.
  
  
  METHODS
  Employing ELISA, RT-PCR or PCR, we evaluated 1216 fecal samples for common diarrheal agents from children aged less than 5-year-old hospitalized with acute gastroenteritis. MEGA software was used to construct phylogenetic trees of the VP1/VP2 partial sequences of the HBoV genome.
  
  
  RESULTS
  There were 67 HBoV-positive specimens, 52 (77.6%) were co-infected with rotavirus, norovirus, astrovirus, or enteric adenovirus. Statistical analysis of the clinical data indicated that children infected with both rotavirus and bocavirus did not have more severe clinical symptoms than children infected with rotavirus. The phylogenetic analysis of the VP1/VP2 partial sequences of the HBoV genome revealed a single genetic lineage.
  
  
  CONCLUSIONS
  Despite its high infection rate, there was no statistically significant a causual relationship between HBoV and gastroenteritis in children.
authors.affiliation: National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
authors.forename: Jie-Mei
authors.initials: JM
authors.lastname: Yu
authors.forename: Dan-Di
authors.initials: DD
authors.lastname: Li
authors.forename: Zi-Qian
authors.initials: ZQ
authors.lastname: Xu
authors.forename: Wei-Xia
authors.initials: WX
authors.lastname: Cheng
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhang
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Li
authors.forename: Shu-Xian
authors.initials: SX
authors.lastname: Cui
authors.lastname: Miao-Jin
authors.forename: Su-Hua
authors.initials: SH
authors.lastname: Yang
authors.forename: Zhao-Yin
authors.initials: ZY
authors.lastname: Fang
authors.forename: Zhao-Jun
authors.initials: ZJ
authors.lastname: Duan
mesh_terms: D000256:Adenoviridae / Q000302:isolation & purification; D052660:Bocavirus / Q000302:isolation & purification*; D002675:Child, Preschool; D002681:China / Q000453:epidemiology; D015897:Comorbidity; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D016679:Genome, Viral; D006760:Hospitalization; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008969:Molecular Sequence Data; D029322:Norovirus / Q000302:isolation & purification; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000821:virology*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D015995:Prevalence; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012401:Rotavirus / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D015678:Viral Structural Proteins / Q000235:genetics
chemical_list: D015678:Viral Structural Proteins