title: [Development of a SPA-ELISA method for detecting anti-coronavirus IgG antibodies in serum samples from fulvous fruit bats].
abstract: OBJECTIVE
  To develop an simple and sensitive method for detecting anti-coronavirus IgG antibodies in bat sera based on enzyme-linked immunosorbent assay (ELISA).
  
  
  METHODS
  A commercial ELISA kit for detecting SARS-CoV antibody was modified for detecting coronavirus antibodies in bat serum samples. The second antibody in the kit was replaced with horseradish peroxidase-conjugated protein-A (HRP-SPA) based on the characteristics of binding between Staphylococcus aureus protein A (SPA) and mammal IgG Fc fragment. The sera of 55 fulvous fruit bats (Rousettus dasymallus) were tested using the SPA-ELISA.
  
  
  RESULTS
  The test results of the positive and negative controls in the kit and the serum samples from convalescent ;patient were consistent with expectation. Coronavirus antibody was detected in 2 out of the 55 bat serum samples. Serum neutralization test confirmed the validity of the SPA-ELISA method.
  
  
  CONCLUSIONS
  This SPA-ELISA method is applicable for detecting coronavirus antibody in bat sera.
authors.affiliation: Department of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. paburaw@hotmail.com
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.forename: Yu-xue
authors.initials: YX
authors.lastname: Liao
authors.forename: Zhong
authors.initials: Z
authors.lastname: Chen
authors.forename: Yu-chun
authors.initials: YC
authors.lastname: Li
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Gao
authors.forename: Yi-xiong
authors.initials: YX
authors.lastname: Chen
authors.forename: Lian-gong
authors.initials: LG
authors.lastname: Cai
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.forename: Shou-yi
authors.initials: SY
authors.lastname: Yu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D002685:Chiroptera / Q000821:virology*; D017934:Coronavirus / Q000276:immunology / Q000302:isolation & purification*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D007074:Immunoglobulin G / Q000097:blood*
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G