title: Catalytic performance of lignin peroxidase in a novel reverse micelle.
abstract: To enhance the catalytic activity of lignin peroxidase (LiP) in a reverse micelle, a synthesized two-tail nonionic surfactant N-gluconyl glutamic acid didecyl ester (GGDE) was used to formulate a novel reverse micelle. Based on the LiP catalyzed oxidation of veratryl alcohol (VA) in this novel GGDE/TritonX-100-cyclohexane-H(2)O reverse micelle, the effects of the size of the reverse micelle, the buffer pH, and the concentration of H(2)O(2) on the catalytic activity of LiP were investigated. Under the optimized conditions, the catalytic efficiency of LiP in the GGDE/TritonX-100 reverse micelle was 40 times higher than that in the AOT reverse micelle. The full expression of catalytic activity of LiP in this medium was mainly due to the lack of electrostatic interaction between LiP and the head group of GGDE and TritonX-100 and to the size fit between LiP and the inner water cavity of the reverse micelle.
authors.affiliation: Key Laboratory of Colloid & Interface Chemistry of the Education Ministry of China, Shandong University, Jinan 250100, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.forename: Xi-Rong
authors.initials: XR
authors.lastname: Huang
authors.forename: Feng
authors.initials: F
authors.lastname: Huang
authors.forename: Yue-Zhong
authors.initials: YZ
authors.lastname: Li
authors.forename: Yin-Bo
authors.initials: YB
authors.lastname: Qu
authors.forename: Pei-Ji
authors.initials: PJ
authors.lastname: Gao
mesh_terms: D002384:Catalysis; D004655:Emulsions; D005942:Gluconates / Q000737:chemistry*; D005971:Glutamates / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008823:Micelles; D017830:Octoxynol / Q000737:chemistry; D010544:Peroxidases / Q000737:chemistry / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry
chemical_list: D004655:Emulsions; D005942:Gluconates; D005971:Glutamates; D008823:Micelles; C531956:N-gluconyl glutamic acid didecyl ester; D013501:Surface-Active Agents; D017830:Octoxynol; D010544:Peroxidases; C042858:lignin peroxidase