Rapid microfabrication of solvent-resistant biocompatible microfluidic devices.
This paper presents a rapid, simple, and low-cost fabrication method to prepare solvent resistant and biocompatible microfluidic devices with three-dimensional geometries. The devices were fabricated in thiolene and replicated from PDMS master with high molding fidelity. Good chemical compatibility for organic solvents allows volatile chemicals in synthesis and analysis applications. The surface can be processed to be hydrophobic or hydrophilic for water-in-oil and oil-in-water emulsions. Monodisperse organic solvent droplet generation is demonstrated to be reproducible in thiolene microchannels without swelling. The thiolene surface prevents cell adhesion but normal cell growth and adhesion on glass substrates is not affected by the adjacent thiolene patterns.
Hung
Lung-Hsin
LH
Department of Biomedical Engineering, University of California at Irvine, 3120 Natural Sciences II, Irvine, CA 92697, USA. lhung@uci.edu
Lin
Robert
R
Lee
Abraham Phillip
AP
D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D018929:Cell Culture Techniques / Q000295:instrumentation / Q000379:methods*; D049109:Cell Proliferation; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D005898:Glass / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D009821:Oils; D012997:Solvents / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013499:Surface Properties; D013997:Time Factors; D014867:Water / Q000737:chemistry
D001672:Biocompatible Materials; D004129:Dimethylpolysiloxanes; D009821:Oils; D012997:Solvents; D013438:Sulfhydryl Compounds; D014867:Water