Antimicrobial resistance of fecal Escherichia coli isolated from grow-finish pigs in 20 herds in Alberta and Saskatchewan.
Escherichia coli (n = 1439), isolated from the feces of apparently healthy grow-finish pigs in 20 herds in Alberta and Saskatchewan, were tested for susceptibility to 16 antimicrobials. All isolates were susceptible to amikacin, ceftriaxone, and ciprofloxacin and less than 1% was resistant to amoxicillin-clavulanic acid, cefoxitin, ceftiofur, gentamicin, and nalidixic acid. Resistance was most common to tetracycline (66.8%), sulfamethoxazole (46.0%) and streptomycin (33.4%). Twenty-one percent of the isolates were susceptible to all drugs, while 57% were resistant to 2 or more antimicrobials. Unconditional associations between resistances provided insight into the potential for co-selection. Every resistance-outcome was associated with at least 2 other drug-resistances. These associations illustrate the propensity for resistance phenotypes to occur together and the importance of considering co-selection in antimicrobial use decisions. A 2nd analysis explored the associations between resistance phenotypes in E. coli and Salmonella spp. from the same herd. Only 2 resistances in Salmonella were associated with herd-level E. coli resistance, indicating that E. coli is a poor sentinel for Salmonella AMR within herds. Herd-level management, including antimicrobial use, could affect antimicrobial resistance. The intra-class correlation between isolates within herds ranged from 0.1 to 0.46, which confirmed resistance clustered within herds. This suggests herd-level interventions might mitigate antimicrobial resistance. Overall, these results reflect the on-farm selection pressure for resistance and the potential food-safety risk from near-market animals. These data provide a baseline for comparisons with future on-farm monitoring of antimicrobial resistance in E. coli.
Rosengren
Leigh B
LB
Department of Large Animal Clinical Sciences, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4. leigh.rosengren@vetepi.com
Waldner
Cheryl L
CL
Reid-Smith
Richard J
RJ
Checkley
Sylvia L
SL
McFall
Margaret E
ME
Rajić
Andrijana
A
D000367:Age Factors; D000416:Alberta; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D015169:Colony Count, Microbial / Q000662:veterinary; D004305:Dose-Response Relationship, Drug; D024881:Drug Resistance, Bacterial; D024901:Drug Resistance, Multiple, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D004927:Escherichia coli Infections / Q000188:drug therapy / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D005260:Female; D008297:Male; D008826:Microbial Sensitivity Tests; D012307:Risk Factors; D012525:Saskatchewan; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000382:microbiology
D000900:Anti-Bacterial Agents