title: The human cerebrospinal fluid metabolome.
abstract: With continuing improvements in analytical technology and an increased interest in comprehensive metabolic profiling of biofluids and tissues, there is a growing need to develop comprehensive reference resources for certain clinically important biofluids, such as blood, urine and cerebrospinal fluid (CSF). As part of our effort to systematically characterize the human metabolome we have chosen to characterize CSF as the first biofluid to be intensively scrutinized. In doing so, we combined comprehensive NMR, gas chromatography-mass spectrometry (GC-MS) and liquid chromatography (LC) Fourier transform-mass spectrometry (FTMS) methods with computer-aided literature mining to identify and quantify essentially all of the metabolites that can be commonly detected (with today's technology) in the human CSF metabolome. Tables containing the compounds, concentrations, spectra, protocols and links to disease associations that we have found for the human CSF metabolome are freely available at http://www.csfmetabolome.ca.
authors.affiliation: Department of Computing Science, University of Alberta, Edmonton, AB, Canada. david.wishart@ualberta.ca
authors.forename: David S
authors.initials: DS
authors.lastname: Wishart
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Lewis
authors.forename: Joshua A
authors.initials: JA
authors.lastname: Morrissey
authors.forename: Mitchel D
authors.initials: MD
authors.lastname: Flegel
authors.forename: Kevin
authors.initials: K
authors.lastname: Jeroncic
authors.forename: Yeping
authors.initials: Y
authors.lastname: Xiong
authors.forename: Dean
authors.initials: D
authors.lastname: Cheng
authors.forename: Roman
authors.initials: R
authors.lastname: Eisner
authors.forename: Bijaya
authors.initials: B
authors.lastname: Gautam
authors.forename: Dan
authors.initials: D
authors.lastname: Tzur
authors.forename: Summit
authors.initials: S
authors.lastname: Sawhney
authors.forename: Fiona
authors.initials: F
authors.lastname: Bamforth
authors.forename: Russ
authors.initials: R
authors.lastname: Greiner
authors.forename: Liang
authors.initials: L
authors.lastname: Li
mesh_terms: D002556:Cerebrospinal Fluid Proteins / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D019295:Computational Biology / Q000379:methods*; D005583:Fourier Analysis; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D019906:Nuclear Magnetic Resonance, Biomolecular
chemical_list: D002556:Cerebrospinal Fluid Proteins