title: Purification of inclusion body-forming peptides and proteins in soluble form by fusion to Escherichia coli thermostable proteins.
abstract: Proteins and peptides expressed in the prokaryotic system often form inclusion bodies. Solubilization and refolding procedures can be used for their recovery, but this process remains difficult. One strategy for improving the solubility of a protein of interest is to fuse it to a highly soluble protein. To select a suitable fusion partner capable of solubilizing the aggregation-prone (inclusion body-forming) proteins and peptides, Escherichia coli thermostable proteins were identified and tested. Among them, trigger factor (TF) protein was selected because of its high expression and stability. Using an expression system based on fusion to TF, selected proteins and peptides that otherwise form inclusion bodies were expressed in soluble state and were purified like other soluble proteins. This system provides a convenient method for production of aggregation-prone proteins and peptides.
authors.affiliation: Chosun University, Gwanju, Republic of Korea.
authors.forename: Arjun
authors.initials: A
authors.lastname: Thapa
authors.forename: Md
authors.initials: M
authors.lastname: Shahnawaz
authors.forename: Pratap
authors.initials: P
authors.lastname: Karki
authors.forename: Giri
authors.initials: G
authors.lastname: Raj Dahal
authors.forename: Md Golam
authors.initials: MG
authors.lastname: Sharoar
authors.forename: Song
authors.initials: S
authors.lastname: Yub Shin
authors.forename: Jung
authors.initials: J
authors.lastname: Sup Lee
authors.forename: Byungyun
authors.initials: B
authors.lastname: Cho
authors.forename: Il-Seon
authors.initials: IS
authors.lastname: Park
mesh_terms: D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D002479:Inclusion Bodies / Q000378:metabolism*; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000302:isolation & purification* / Q000378:metabolism*; D012995:Solubility; D013696:Temperature
chemical_list: D029968:Escherichia coli Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins