A collagen-binding mimetic of neural cell adhesion molecule.
A chimeric protein consisting of a cell-adhesive peptide derived from a neural cell adhesion molecule and a collagen-binding domain was synthesized using recombinant DNA technology. Here, we demonstrate that the chimeric protein binds to type I collagen and promotes the adhesion and neurite extension of hippocampus neurons. These results suggest that the chimeric protein has potential to provide microenvironments for neurons to adhere and survive in collagen-based matrices for use in cell-based therapies for central nervous disorders.
Miyazaki
Hiroko
H
Department of Reparative Materials, Graduate School of Engineering, Kyoto University, Kyoto, Japan.
Kato
Koichi
K
Teramura
Yuji
Y
Iwata
Hiroo
H
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005240:Feasibility Studies; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D051379:Mice; D019006:Neural Cell Adhesion Molecules / Q000737:chemistry* / Q000378:metabolism*; D016501:Neurites / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000737:chemistry* / Q000378:metabolism*; D020349:Surface Plasmon Resonance
D019006:Neural Cell Adhesion Molecules; D010455:Peptides; D011993:Recombinant Fusion Proteins; D003094:Collagen