title: Identification and antimicrobial susceptibility testing of Staphylococcus vitulinus by the BD phoenix automated microbiology system.
abstract: This study evaluated the performance of the BD Phoenix system for the identification (ID) and antimicrobial susceptibility testing (AST) of Staphylococcus vitulinus. Of the 10 S. vitulinus isolates included in the study, 2 were obtained from the Czech Collection of Microorganisms, 5 from the environment, 2 from human clinical samples, and 1 from an animal source. The results of conventional biochemical and molecular tests were used for the reference method for ID, while antimicrobial susceptibility testing performed in accordance with Clinical and Laboratory Standards Institute recommendations and PCR for the mecA gene were the reference for AST. Three isolates were incorrectly identified by the BD Phoenix system; one of these was incorrectly identified to the genus level, and two to the species level. The results of AST by the BD Phoenix system were in agreement with those by the reference method used. While the results of susceptibility testing compared favorably, the 70% accuracy of the Phoenix system for identification of this unusual staphylococcal species was not fully satisfactory.
authors.affiliation: Department of Bacteriology, Institute of Microbiology and Immunology, School of Medicine, University of Belgrade, Dr. Subotića 1, 11000 Belgrade, Serbia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Cirković
authors.forename: Tomasz
authors.initials: T
authors.lastname: Hauschild
authors.forename: Petr
authors.initials: P
authors.lastname: Jezek
authors.forename: Vladimir
authors.initials: V
authors.lastname: Dimitrijević
authors.forename: Dragana
authors.initials: D
authors.lastname: Vuković
authors.forename: Srdjan
authors.initials: S
authors.lastname: Stepanović
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001331:Automation; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D001431:Bacteriological Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000235:genetics; D004783:Environmental Microbiology; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D046915:Penicillin-Binding Proteins; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus