Oxygen vectors used for S-adenosylmethionine production in recombinant Pichia pastoris with sorbitol as supplemental carbon source.
In order to increase the yield of S-adenosylmethionine (SAM) in recombinant Pichia pastoris, a strategy of adding oxygen vectors and supplemental carbon sources was described. Three organic solutions were used as oxygen vectors for SAM accumulation at different concentrations and addition times. Firstly, n-hexane (0.5%) or n-heptane (1.0%) was added after 72 h of cultivation to improve SAM production. Carbon metabolism was scarce during the induction phase because of low methanol concentration. Secondly, sorbitol (1.2%), selected from three candidates (glycerol, lactic acid, and sorbitol), was used as the supplemental carbon source. The yield of SAM was improved significantly (53.26%) at 1.0%n-heptane added at 72 h (48 h induction), 1.2% sorbitol added at 72, 96, and 120 h of cultivation and 1.0% methanol added every 24 h during cultivation.
Zhang
Jian-Guo
JG
State Key Laboratory of Bioreactor Engineering, College of Biotechnology, East China University of Science and Technology, No. 130 Meilong Road, Shanghai 200237, People's Republic of China.
Wang
Xue-Dong
XD
Zhang
Ji-Ning
JN
Wei
Dong-Zhi
DZ
D003470:Culture Media / Q000737:chemistry; D015870:Gene Expression; D008716:Methionine Adenosyltransferase / Q000096:biosynthesis* / Q000235:genetics; D010843:Pichia / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D012436:S-Adenosylmethionine / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis* / Q000235:genetics; D013012:Sorbitol / Q000737:chemistry*; D013997:Time Factors
D003470:Culture Media; D011994:Recombinant Proteins; D029701:Saccharomyces cerevisiae Proteins; D013012:Sorbitol; D012436:S-Adenosylmethionine; D008716:Methionine Adenosyltransferase