title: Biological selection of peptides for poly(l-lactide) substrates.
abstract: Short peptides that recognize the alpha form of poly( l-lactide) (PLLA) crystalline films were identified from a phage-displayed peptide library. An enzyme-linked immunosorbent assay (ELISA) revealed that the apparent binding constants of the phage clones for the alpha form of PLLA were greater than those of the unselected phage library. The specificity index for the alpha form of PLLA referred to a structurally similar atactic poly(methyl methacrylate) (at-PMMA), supporting the alpha form of PLLA specific binding of the selected phage. Amino acid residues with proton-donor lateral groups and hydrophobic alkyl groups were relatively enriched in a sequence of heptapeptides on the specific phage clones, thereby suggesting the presence of hydrogen bonding as well as hydrophobic interactions between the alpha form of PLLA and the peptides. Surface plasmon resonance (SPR) analysis revealed that the binding constant of the freed c22 heptapeptide (Gln-Leu-Met-His-Asp-Tyr-Arg) for the alpha form of PLLA was greater than those for reference at-PMMA, amorphous PLLA, and the beta form of PLLA. It was found that c22 peptide can recognize slight differences in PLLA polymorphs such as a crystalline state and an arrangement of PLLA functional groups.
authors.affiliation: Komaba Open Laboratory, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.
authors.forename: Hisao
authors.initials: H
authors.lastname: Matsuno
authors.forename: Jun
authors.initials: J
authors.lastname: Sekine
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Yajima
authors.forename: Takeshi
authors.initials: T
authors.lastname: Serizawa
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D007700:Kinetics; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013696:Temperature
chemical_list: D000596:Amino Acids; D019151:Peptide Library; D010455:Peptides; D011091:Polyesters; C033616:poly(lactide)