Filamentous vibriophage fs2 encoding the rstC gene integrates into the same chromosomal region as the CTX phage [corrected].
The genome of the filamentous phage of Vibrio cholerae fs2 was found to contain rstC and rstB1 (truncated) genes downstream of ORF500. att-fs2-dir and att-fs2-rev sequences homologous to that of att-CTXphi were found between orf500 and rstC of the fs2 genome. This prompted us to search for the integration site of fs2 in the genomes of V. cholerae O1 and O139. The genome of fs2 was found to integrate downstream of attRS of the CTXphi phage, which integrated into chromosome I of V. cholerae O1 and O139. When infected with fs2, a fimbriate strain of V. cholerae O1 appeared to reduce fimbrial production in an adult rabbit ileal loop assay.
Nguyen
Dong Tu
DT
Department of Bacteriology, National Institute of Hygiene and Epidemiology, Hanoi, Vietnam.
Nguyen
Binh Minh
BM
Tran
Huy Hoang
HH
Ngo
Tuan Cuong
TC
Le
Thanh Huong
TH
Nguyen
Hoai Thu
HT
Albert
M John
MJ
Iwami
Mamoru
M
Ehara
Masahiko
M
D000818:Animals; D001287:Attachment Sites, Microbiological; D001483:Base Sequence; D002876:Chromosomes, Bacterial / Q000821:virology; D004269:DNA, Bacterial / Q000302:isolation & purification; D010861:Fimbriae, Bacterial / Q000821:virology; D016679:Genome, Viral; D007082:Ileum / Q000821:virology; D017912:Inovirus / Q000235:genetics*; D008242:Lysogeny; D008297:Male; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D011817:Rabbits; D017422:Sequence Analysis, DNA; D041241:Vibrio cholerae O1 / Q000821:virology*; D041242:Vibrio cholerae O139 / Q000821:virology*
D004269:DNA, Bacterial