title: Growth monitoring of Fibrobacter succinogenes by pressure measurement.
abstract: In life support systems, such as the MELiSSA (Micro-Ecological Life Support Alternative) project, developed by the European Space Agency, the aim is to understand and assemble artificial ecosystems for ensuring human subsistence in space. Fibrobacter succinogenes, an anaerobic bacterium, was used for the degradation of vegetable wastes produced in higher plants chambers, but the process does not allow the monitoring of biomass concentration and degradation rates. This study proposes a growth and a degradation monitoring technique using pressure measurements. First, volatile fatty acids (VFA) production was compared with biomass growth and with CO(2) production. The experiments were carried out in batch and fed-batch processes on glucose and on vegetables. The results have shown that a link could be established between VFA production, degradation rate and gas pressure measurements. Thus, the pressure could be used both as a relevant variable for online evaluation of biomass growth and of degradation of complex vegetable wastes.
authors.affiliation: Laboratoire de Génie Chimique et Biochimique, Polytech' Clermont-Ferrand, Université Blaise Pascal, Aubière Cedex, France. gwendoline.christophe@univ-bpclermont.fr
authors.forename: G
authors.initials: G
authors.lastname: Christophe
authors.forename: E
authors.initials: E
authors.lastname: Guiavarch
authors.forename: C
authors.initials: C
authors.lastname: Creuly
authors.forename: C-G
authors.initials: CG
authors.lastname: Dussap
mesh_terms: D018533:Biomass; D001709:Biotechnology / Q000379:methods*; D001937:Brassica / Q000378:metabolism; D002245:Carbon Dioxide / Q000737:chemistry; D004867:Equipment Design; D005285:Fermentation; D045848:Fibrobacter / Q000378:metabolism*; D005740:Gases; D005947:Glucose / Q000737:chemistry; D006801:Humans; D007218:Industrial Microbiology / Q000379:methods*; D011312:Pressure; D013025:Glycine max / Q000378:metabolism; D013997:Time Factors; D014908:Triticum / Q000378:metabolism
chemical_list: D005740:Gases; D002245:Carbon Dioxide; D005947:Glucose