title: Different region analysis for genotyping Yersinia pestis isolates from China.
abstract: BACKGROUND
  DFR (different region) analysis has been developed for typing Yesinia pestis in our previous study, and in this study, we extended this method by using 23 DFRs to investigate 909 Chinese Y. pestis strains for validating DFR-based genotyping method and better understanding adaptive microevolution of Y. pestis.
  
  
  RESULTS
  On the basis of PCR and Bionumerics data analysis, 909 Y. pestis strains were genotyped into 32 genomovars according to their DFR profiles. New terms, Major genomovar and Minor genomovar, were coined for illustrating evolutionary relationship between Y. pestis strains from different plague foci and different hosts. In silico DFR profiling of the completed or draft genomes shed lights on the evolutionary scenario of Y. pestis from Y. pseudotuberculosis. Notably, several sequenced Y. pestis strains share the same DFR profiles with Chinese strains, providing data for revealing the global plague foci expansion.
  
  
  CONCLUSIONS
  Distribution of Y. pestis genomovars is plague focus-specific. Microevolution of biovar Orientalis was deduced according to DFR profiles. DFR analysis turns to be an efficient and inexpensive method to portrait the genome plasticity of Y. pestis based on horizontal gene transfer (HGT). DFR analysis can also be used as a tool in comparative and evolutionary genomic research for other bacteria with similar genome plasticity.
authors.affiliation: Laboratory of Analytical Microbiology, State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Beijing, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Li
authors.forename: Erhei
authors.initials: E
authors.lastname: Dai
authors.forename: Yujun
authors.initials: Y
authors.lastname: Cui
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.forename: Yujiang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Mingshou
authors.initials: M
authors.lastname: Wu
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Zhou
authors.forename: Zhaobiao
authors.initials: Z
authors.lastname: Guo
authors.forename: Xiang
authors.initials: X
authors.lastname: Dai
authors.forename: Baizhong
authors.initials: B
authors.lastname: Cui
authors.forename: Zhizhen
authors.initials: Z
authors.lastname: Qi
authors.forename: Zuyun
authors.initials: Z
authors.lastname: Wang
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.forename: Xingqi
authors.initials: X
authors.lastname: Dong
authors.forename: Zhizhong
authors.initials: Z
authors.lastname: Song
authors.forename: Junhui
authors.initials: J
authors.lastname: Zhai
authors.forename: Yajun
authors.initials: Y
authors.lastname: Song
authors.forename: Ruifu
authors.initials: R
authors.lastname: Yang
mesh_terms: D001483:Base Sequence; D002681:China; D017931:DNA Primers; D005798:Genes, Bacterial; D005838:Genotype; D016133:Polymerase Chain Reaction; D015010:Yersinia pestis / Q000235:genetics*; D015011:Yersinia pseudotuberculosis / Q000235:genetics
chemical_list: D017931:DNA Primers