High-level expression of recombinant human nerve growth factor beta in milk of nontransgenic rabbits.
The technology for the large-scale production of therapeutic recombinant proteins remains a challenge in the biopharmaceutical industry. In this study, we reported a nontransgenic approach to producing a large quantity of human nerve growth factor beta (hNGF-beta) in rabbit milk by employing a recombinant adenoviral expression system. After directly instilling hNGF-beta recombinant adenoviruses into rabbit mammary glands, a polypeptide with a molecular weight of 13.2 kDa was detected in rabbit milk. The maximal expression level of hNGF-beta reached 346 mug/ml. The biological activity of recombinant hNGF-beta was confirmed using PC12 cells and cultures of dorsal root ganglion neurons from chicken embryos. Our data suggest that instilling recombinant adenovirus directly into the mammary gland of mammals is an efficient approach to producing a large quantity of hNGF-beta.
Xiao
Bo
B
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, P.R. China.
Li
Qing Wang
QW
Feng
Bo
B
Han
Zeng Sheng
ZS
Gao
Da Wei
DW
Li
Jian
J
Li
Kun
K
Zhao
Rui
R
Jiang
Zhong Liang
ZL
Hu
Jian Hong
JH
Zhi
Xu Bo
XB
D000256:Adenoviridae; D000818:Animals; D002642:Chick Embryo; D005260:Female; D005727:Ganglia, Spinal / Q000254:growth & development; D015870:Gene Expression; D006801:Humans; D008321:Mammary Glands, Animal / Q000378:metabolism*; D008892:Milk / Q000378:metabolism*; D020932:Nerve Growth Factor / Q000096:biosynthesis* / Q000494:pharmacology; D016716:PC12 Cells; D011817:Rabbits; D051381:Rats; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000494:pharmacology; D014161:Transduction, Genetic
D011994:Recombinant Proteins; D020932:Nerve Growth Factor