title: Effect of platelet activating factor antagonist treatment on gentamicin nephrotoxicity.
abstract: To assess whether PAF could be involved in the gentamicin-induced nephrotoxicity, we have studied the effect of PAF antagonist BN-52021 on renal function in rats after gentamicin (GENTA) treatment. Experiments were completed in 21 Wistar rats divided into three groups: group GENTA was injected with gentamicin 100 mg kg(-1) body wt/day s.c. for 6 days. Group GENTA + BN received gentamicin and BN-52021 i.p. 5 mg kg(-1) body wt/day. A third group served as control. Rats were placed in meta-bolic cages and plasma creatinine and creatinine clearance were measured daily. GENTA group showed a progressive increase in plasma creatinine, a drop in creatinine clearance and an increase in urinary excretion of N-acetyl-beta-D-glucosaminidase and alkaline phosphatase. GENTA + BN group showed a lesser change in plasma creatinine and a creatinine clearance, but no difference with GENTA group in urinary excretion of NAG and AP were observed. Histological examination revealed a massive cortical tubular necrosis in rats treated with gentamicin, whereas in BN-52021 injected animals tubular damage was markedly attenuated. The present results suggest a role for PAF in the gentamicininduced nephro-toxicity.
authors.affiliation: Departmento de Fisiologia y Farmacologia Facultad de Medicina Universidad de Salamanca Avda, Campo Charro s/n salamanca 37007 Spain.
authors.forename: A
authors.initials: A
authors.lastname: Rodriguez-Barbero
authors.forename: E
authors.initials: E
authors.lastname: Bosque
authors.forename: L
authors.initials: L
authors.lastname: Rivas-Cabaero
authors.forename: M
authors.initials: M
authors.lastname: Arévalo
authors.forename: J M
authors.initials: JM
authors.lastname: López-Novoa