title: Poly(N-isopropylacrylamide) grafted on plasma-activated poly(ethylene oxide): thermal response and interaction with proteins.
abstract: Thermoresponsive polymer layers offer the possibility of preparing smart surfaces with properties that are switchable through a phase transition, usually close to the lower critical solution temperature of the polymer. In particular, poly( N-isopropylacrylamide) (pNIPAM) has gained a great deal of attention because it has such a phase transition in a physiologically interesting temperature range. We have prepared ultrathin thermoresponsive coatings by grafting pNIPAM on a plasma-CVD-deposited, poly(ethylene oxide)-like polymer substrate that was activated in an Ar plasma discharge to initiate the grafting. The presence and integrity of pNIPAM was verified by XPS and ToF-SIMS, and a dramatic change in the wettability during the phase transition was identified by temperature-dependent contact angle measurements. The transition from the hydrated to the collapsed conformation was analyzed by temperature-dependent QCM measurements and by AFM. An unusual, reversible behavior of the viscoelastic properties was seen directly at the phase transition from the swollen to the collapsed state. The phase transition leads to a switching from protein repulsion to a state that allows the adsorption of proteins.
authors.affiliation: Institute for Health and Consumer Protection, Directorate General Joint Research Centre, European Commission, Via E. Fermi, 21027 Ispra (VA), Italy.
authors.forename: P
authors.initials: P
authors.lastname: Heinz
authors.forename: F
authors.initials: F
authors.lastname: Brétagnol
authors.forename: I
authors.initials: I
authors.lastname: Mannelli
authors.forename: L
authors.initials: L
authors.lastname: Sirghi
authors.forename: A
authors.initials: A
authors.lastname: Valsesia
authors.forename: G
authors.initials: G
authors.lastname: Ceccone
authors.forename: D
authors.initials: D
authors.lastname: Gilliland
authors.forename: K
authors.initials: K
authors.lastname: Landfester
authors.forename: H
authors.initials: H
authors.lastname: Rauscher
authors.forename: F
authors.initials: F
authors.lastname: Rossi
mesh_terms: D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins; D000327:Adsorption; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D005027:Ethylene Oxide / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008968:Molecular Conformation; D011108:Polymers / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D013052:Spectrometry, X-Ray Emission; D013499:Surface Properties; D013696:Temperature; D014965:X-Rays
chemical_list: D000178:Acrylamides; D000180:Acrylic Resins; D001672:Biocompatible Materials; D011108:Polymers; D011506:Proteins; D012709:Serum Albumin; C052970:poly-N-isopropylacrylamide; D005027:Ethylene Oxide