Highly efficient depletion strategy for the two most abundant erythrocyte soluble proteins improves proteome coverage dramatically.
In-depth human erythrocyte proteome studies are severely hampered by the presence of hemoglobin and carbonic anhydrase-1, which account for more than 98% of the total erythrocyte soluble protein content. We developed a specific depletion approach that resulted in a drastic increase in the number of identified proteins. This depletion technique is valuable for proteome studies of human erythrocyte disorders with unknown etiology and of tissue samples that contain blood.
Ringrose
Jeffrey H
JH
Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.
van Solinge
Wouter W
WW
Mohammed
Shabaz
S
O'Flaherty
Martina C
MC
van Wijk
Richard
R
Heck
Albert J R
AJ
Slijper
Monique
M
D001798:Blood Proteins / Q000032:analysis*; D024401:Carbonic Anhydrase I / Q000097:blood / Q000302:isolation & purification; D004912:Erythrocytes / Q000737:chemistry*; D006454:Hemoglobins / Q000302:isolation & purification; D006801:Humans; D020543:Proteome / Q000032:analysis*; D012995:Solubility
D001798:Blood Proteins; D006454:Hemoglobins; D020543:Proteome; D024401:Carbonic Anhydrase I