title: Interactions between PBEF and oxidative stress proteins--a potential new mechanism underlying PBEF in the pathogenesis of acute lung injury.
abstract: Identification of pre-B-cell colony-enhancing factor (PBEF) interacting partners may reveal new molecular mechanisms of PBEF in the pathogenesis of acute lung injury (ALI). The interactions between PBEF and NADH dehydrogenase subunit 1(ND1), ferritin light chain and interferon induced transmembrane 3 (IFITM3) in human pulmonary vascular endothelial cells were identified and validated. ND1, ferritin and IFITM3 are involved in oxidative stress and inflammation. Overexpression of PBEF increased its interactions and intracellular oxidative stress, which can be attenuated by rotenone. The interaction modeling between PBEF and ND1 is consistent with the corresponding experimental finding. These interactions may underlie a novel role of PBEF in the pathogenesis of ALI.
authors.affiliation: Department of Surgery, University of Missouri, Columbia, MO, USA.
authors.forename: Li Qin
authors.initials: LQ
authors.lastname: Zhang
authors.forename: Djanybek M
authors.initials: DM
authors.lastname: Adyshev
authors.forename: Patrick
authors.initials: P
authors.lastname: Singleton
authors.forename: Hailong
authors.initials: H
authors.lastname: Li
authors.forename: Javier
authors.initials: J
authors.lastname: Cepeda
authors.forename: Sheng-You
authors.initials: SY
authors.lastname: Huang
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: Zou
authors.forename: Alexander D
authors.initials: AD
authors.lastname: Verin
authors.forename: Jiancheng
authors.initials: J
authors.lastname: Tu
authors.forename: Joe G N
authors.initials: JG
authors.lastname: Garcia
authors.forename: Shui Qing
authors.initials: SQ
authors.lastname: Ye
mesh_terms: D000595:Amino Acid Sequence; D016207:Cytokines / Q000737:chemistry / Q000378:metabolism*; D005293:Ferritins / Q000378:metabolism*; D015723:Gene Library; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D054409:Nicotinamide Phosphoribosyltransferase / Q000737:chemistry / Q000378:metabolism*; D018384:Oxidative Stress; D011487:Protein Conformation; D016601:RNA-Binding Proteins / Q000378:metabolism*; D012128:Respiratory Distress Syndrome / Q000209:etiology* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D016207:Cytokines; C080082:IFITM3 protein, human; D008565:Membrane Proteins; D016601:RNA-Binding Proteins; D005293:Ferritins; D009245:NADH Dehydrogenase; C515075:NADH dehydrogenase subunit 1, human; D054409:Nicotinamide Phosphoribosyltransferase; C085455:nicotinamide phosphoribosyltransferase, human