Chemical site-selective prenylation of proteins.
A direct thionation procedure allows conversion of allylic alcohols into the corresponding thiols, the products of which are immediately compatible with one-pot site-selective selenenyl sulfide mediated protein conjugation.
Gamblin
David P
DP
Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Mansfield Road, Oxford, UK.
van Kasteren
Sander
S
Bernardes
Gonçalo J L
GJ
Chalker
Justin M
JM
Oldham
Neil J
NJ
Fairbanks
Antony J
AJ
Davis
Benjamin G
BG
D001407:Bacillus / Q000201:enzymology; D001665:Binding Sites; D057927:Hydrophobic and Hydrophilic Interactions; D015394:Molecular Structure; D020005:Propanols / Q000737:chemistry*; D017368:Protein Prenylation; D011994:Recombinant Proteins / Q000138:chemical synthesis / Q000737:chemistry; D018036:Selenium Compounds / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D020860:Subtilisin / Q000138:chemical synthesis / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000138:chemical synthesis* / Q000737:chemistry
D020005:Propanols; D011994:Recombinant Proteins; D018036:Selenium Compounds; D013438:Sulfhydryl Compounds; C006463:allyl alcohol; D020860:Subtilisin