title: Impact of freezing on high-density lipoprotein functionality.
abstract: Structural and functional properties of high-density lipoprotein (HDL) after short-term freezing in the presence or absence of 10% sucrose were compared with HDL stored at 4 degrees C. Freezing did not affect the size of HDL particles or their antiinflammatory and antioxidant properties. Freezing slightly impaired the ability of HDL to support cholesterol efflux from human macrophages, but this property was preserved when HDL was frozen in the presence of sucrose. Freezing also resulted in approximately 10% loss of HDL in the samples. We conclude that freezing HDL in the presence of 10% sucrose preserves its structural and functional properties.
authors.affiliation: Baker Heart Research Institute, Melbourne, VIC 8008, Australia.
authors.forename: J Rajinda
authors.initials: JR
authors.lastname: Kekulawala
authors.forename: Andrew
authors.initials: A
authors.lastname: Murphy
authors.forename: Wilissa
authors.initials: W
authors.lastname: D'Souza
authors.forename: Chun
authors.initials: C
authors.lastname: Wai
authors.forename: Jaye
authors.initials: J
authors.lastname: Chin-Dusting
authors.forename: Bronwyn
authors.initials: B
authors.lastname: Kingwell
authors.forename: Dmitri
authors.initials: D
authors.lastname: Sviridov
authors.forename: Nigora
authors.initials: N
authors.lastname: Mukhamedova
mesh_terms: D005615:Freezing; D006801:Humans; D008075:Lipoproteins, HDL / Q000737:chemistry* / Q000378:metabolism*; D010316:Particle Size; D013395:Sucrose / Q000494:pharmacology
chemical_list: D008075:Lipoproteins, HDL; D013395:Sucrose