title: Effect of mineral trioxide aggregate on dentin bridge formation and expression of dentin sialoprotein and heme oxygenase-1 in human dental pulp.
abstract: This study was conducted to evaluate the pulpal response to direct capping with either mineral trioxide aggregate (MTA) or calcium hydroxide (CH) cement in humans, with a focus on dentin bridge formation and dentin sialoprotein (DSP) and heme oxygenase-1 (HO-1) expression. Direct pulp capping was performed in 20 cases of caries-free human third molars. The pulps were exposed and capped with either MTA or hard-setting CH. After 2 months, the teeth were extracted, and the specimens were prepared for histologic and immunohistochemical evaluations. Histologically, 100% of the MTA group and 60% of the CH group developed dentin bridges. The mean thickness of the dentin bridges observed in the MTA group was statistically greater than that of CH group. In addition, DSP and HO-1 were expressed in the odontoblast-like cells and pulp fibroblasts beneath the dentin bridge; furthermore, significantly greater immunostaining was observed in the MTA group than in the CH group. Collectively, these results indicate that MTA is superior to CH in terms of inducing the dentinogenic process in human pulp capping.
authors.affiliation: Department of Conservative Dentistry, College of Dentistry, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Kyung-San
authors.initials: KS
authors.lastname: Min
authors.forename: Hyo-Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Sun-Kyung
authors.initials: SK
authors.lastname: Lee
authors.forename: Sang-Hyuk
authors.initials: SH
authors.lastname: Park
authors.forename: Chan-Ui
authors.initials: CU
authors.lastname: Hong
authors.forename: Hae-Won
authors.initials: HW
authors.lastname: Kim
authors.forename: Hae-Hyoung
authors.initials: HH
authors.lastname: Lee
authors.forename: Eun-Cheol
authors.initials: EC
authors.lastname: Kim
mesh_terms: D000328:Adult; D017607:Aluminum Compounds / Q000494:pharmacology*; D017610:Calcium Compounds / Q000494:pharmacology*; D002126:Calcium Hydroxide / Q000494:pharmacology; D019610:Cytoprotection; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D003785:Dental Pulp Capping / Q000379:methods*; D003809:Dentin, Secondary / Q000254:growth & development / Q000378:metabolism*; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis* / Q000502:physiology; D006801:Humans; D007124:Immunoenzyme Techniques; D008875:Middle Aged; D008964:Molar, Third; D009804:Odontoblasts / Q000378:metabolism; D010087:Oxides / Q000494:pharmacology*; D010750:Phosphoproteins; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D012795:Sialoglycoproteins; D017640:Silicates / Q000494:pharmacology*; D000099311:Dentin Sialophosphoprotein
chemical_list: D017607:Aluminum Compounds; D017610:Calcium Compounds; D002126:Calcium Hydroxide; D004338:Drug Combinations; D016326:Extracellular Matrix Proteins; D051547:Heme Oxygenase-1; D010087:Oxides; D010750:Phosphoproteins; D012387:Root Canal Filling Materials; D012795:Sialoglycoproteins; D017640:Silicates; D000099311:Dentin Sialophosphoprotein; C086631:mineral trioxide aggregate