title: Decoration of superparamagnetic iron oxide nanoparticles with Ni2+: agent to bind and separate histidine-tagged proteins.
abstract: The decoration of iron oxide nanoparticles with Ni2+ ions provided the superparamagnetic nanoparticles with a binding site for His-tagged proteins, allowing their selective binding and convenient separation from a multi-component solution with an appropriately applied magnetic field.
authors.affiliation: Department of Chemistry & Advanced Material Sciences, College of Environment and Applied Chemistry, Kyung Hee University, Gyeonggido 449-701, Korea.
authors.forename: Kyung Sig
authors.initials: KS
authors.lastname: Lee
authors.forename: In Su
authors.initials: IS
authors.lastname: Lee
mesh_terms: D005290:Ferric Compounds / Q000737:chemistry*; D005453:Fluorescence; D006639:Histidine / Q000737:chemistry*; D008280:Magnetics; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D005290:Ferric Compounds; D011506:Proteins; C000499:ferric oxide; D006639:Histidine; D009532:Nickel