[Efficacy of various methods of a reporter gene transfer to chicken embryonic cells].
The methods of transfection ofa plasmid with a reporter gene involving DNA injection into chicken embryonic cells were studied. The parameters of the efficient transfection of chicken blastodermal cells with a foreign gene have been determined (20-24 and up to 40% in culture and embryos, respectively). A high efficiency of transfection of primordial germ cells isolated from the gonads has been obtained after DNA injection into the dorsal aorta of 2.5-day-old chicken embryos.
Suraeva
N M
NM
Baryshnikov
A Iu
AIu
Fisinin
V I
VI
Prokof'ev
M I
MI
D000818:Animals; D030801:Animals, Genetically Modified; D001011:Aorta; D001756:Blastoderm / Q000166:cytology; D002478:Cells, Cultured; D002642:Chick Embryo / Q000196:embryology / Q000201:enzymology*; D017930:Genes, Reporter; D006066:Gonads / Q000166:cytology; D009928:Organ Specificity; D010957:Plasmids; D014162:Transfection / Q000379:methods*; D001616:beta-Galactosidase / Q000235:genetics* / Q000378:metabolism
D001616:beta-Galactosidase