[Nonsteroidal anti-inflammatory drug NS398 regulates the RECK gene expression in the prostate carcinoma strain DU145].
OBJECTIVE
To investigate the regulative effect of the nonsteroidal anti-inflammatory drug NS398 on the RECK gene in the prostate carcinoma strain DU145.


METHODS
DU145 was treated with various concentrations of NS398 for 48 hours. The mRNA level was measured by RT PCR technique and the expression of the RECK protein determined by Western blot.


RESULTS
The mRNA level of the RECK gene was obviously higher, while the MMP9 level markedly lower in the treated group than in the control, and so was the expression of the RECK protein.


CONCLUSIONS
NS398 induces the expression of the RECK gene, which might be the mechanism of its anti-tumor effect.
Xu
Zhen-Yu
ZY
Department of Urology, Nanjing General Hospital of Nanjing Military Command, PLA, Nanjing, Jiangsu 210002, China. dr_zhenyu1976@yahoo.com.cn
Gao
Jian-Ping
JP
Sun
Ying-Hao
YH
Zhang
Zheng-Yu
ZY
Ge
Jing-Ping
JP
Xu
Chuan-Liang
CL
Wang
Lin-Hui
LH
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D015153:Blotting, Western; D002417:Cattle; D045744:Cell Line, Tumor; D058851:GPI-Linked Proteins; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis / Q000235:genetics*; D009578:Nitrobenzenes / Q000494:pharmacology*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013449:Sulfonamides / Q000494:pharmacology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D058851:GPI-Linked Proteins; D008562:Membrane Glycoproteins; D009578:Nitrobenzenes; C115460:RECK protein, human; D012333:RNA, Messenger; D013449:Sulfonamides; C080955:N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide