title: Host-cell-dependent role of actin cytoskeleton during the replication of a human strain of influenza A virus.
abstract: This study was aimed at investigating the possible involvement of the actin cytoskeleton in the modulation of host permissiveness to A/NWS/33 human influenza virus infection in two mammalian (MDCK and LLC-MK2) cell lines in vitro. During the early stages of infection, no appreciable association between incoming NWS/33 virions and cortical actin was detectable in the permissive MDCK model by confocal microscopy, while extensive colocalization and a slower infection progression were observed in LLC-MK2 cells. In the latter model, we also demonstrated the inability of the virus to carry out multiple replication cycles, irrespective of the presence of cleaved HA subunits in the released virions. Treatment with the actin-depolymerizing agent cytochalasin D significantly increased the infection efficiency in LLC-MK2 cells, while a detrimental effect was observed in the MDCK cell line. Our data suggest a selective role of the actin network in inducing a restriction to influenza virus replication, mostly depending on its molecular organization, the host cell type and virus replication phase.
authors.affiliation: Microbiology Section, Department of Pathology and Laboratory Medicine, University of Parma, viale Antonio Gramsci 14, 43100 Parma, Italy. mariacristina.arcangeletti@unipr.it
authors.forename: M C
authors.initials: MC
authors.lastname: Arcangeletti
authors.forename: F
authors.initials: F
authors.lastname: De Conto
authors.forename: F
authors.initials: F
authors.lastname: Ferraglia
authors.forename: F
authors.initials: F
authors.lastname: Pinardi
authors.forename: R
authors.initials: R
authors.lastname: Gatti
authors.forename: G
authors.initials: G
authors.lastname: Orlandini
authors.forename: S
authors.initials: S
authors.lastname: Covan
authors.forename: F
authors.initials: F
authors.lastname: Motta
authors.forename: I
authors.initials: I
authors.lastname: Rodighiero
authors.forename: G
authors.initials: G
authors.lastname: Dettori
authors.forename: C
authors.initials: C
authors.lastname: Chezzi
mesh_terms: D000199:Actins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D000818:Animals; D002460:Cell Line; D015638:Cytochalasin D / Q000494:pharmacology; D003599:Cytoskeleton / Q000378:metabolism / Q000821:virology*; D004285:Dogs; D009980:Influenza A virus / Q000502:physiology*; D008253:Macaca mulatta; D018613:Microscopy, Confocal; D014779:Virus Replication
chemical_list: D000199:Actins; D015638:Cytochalasin D