title: [Molecular cloning and preliminary function study of a novel human gene AC3-33 related to suppress AP-1 activity].
abstract: Activator protein-1(AP-1) is an important transcription factor, and dysregulation of its activity has been associated with many human diseases, including various cancers. A novel human gene AC3-33 (GenBank name: c30rf33, Accession No. FLJ31139), which can suppress PMA and Ionomycin induced activation of AP-1, was identified from 650 human func-tion-known genes by using the high throughput-high content cell-based screening technology. The gene whose full cDNA length is 1391 bp containing 6 exons and 5 introns is located in the human chromosome 3q25.31. The predicted protein encoded by this gene contains 251 amino acids with a theoretical molecular weight of 29 kDa. Expression of the AC3-33 gene is widly found in adrenal glands and cervix. The amino acid sequences of AC3-33 is highly conserved, and has no homology to other known proteins. Subcellular localization studies show that the AC3-33 protein was localized in the cytoplasm. Our preliminary results showed that AC3-33 is an important novel gene related to supress AP-1 activity.
authors.affiliation: Department of Biology, Northchina Coal Medical College, Tangshan 063000, China. panpan424@163.com
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Deng
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.forename: Yang
authors.initials: Y
authors.lastname: Lu
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.forename: Ming
authors.initials: M
authors.lastname: Li
authors.forename: Jie
authors.initials: J
authors.lastname: Zhao
authors.forename: Tai-Ping
authors.initials: TP
authors.lastname: Shi
authors.forename: Xiu-Jun
authors.initials: XJ
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D015152:Blotting, Northern; D002460:Cell Line; D003001:Cloning, Molecular; D005091:Exons / Q000235:genetics; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007438:Introns / Q000235:genetics; D015759:Ionomycin / Q000494:pharmacology; D008856:Microscopy, Fluorescence; D008969:Molecular Sequence Data; D010802:Phylogeny; D011109:Polymethacrylic Acids / Q000494:pharmacology; D011506:Proteins / Q000737:chemistry / Q000145:classification / Q000378:metabolism* / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment; D018808:Transcription Factor AP-1 / Q000235:genetics*
chemical_list: D011109:Polymethacrylic Acids; D011506:Proteins; D018808:Transcription Factor AP-1; D015759:Ionomycin