[Effect of Tongxinluo on the proliferation and differentiation of rat embryonic neural stem cells].
OBJECTIVE
To investigate the effect of Tongxinluo in on the proliferation and differentiation of rat embryonic neural stem cells (NSCs).


METHODS
NSCs were isolated from 12- to 14-day SD rat embryo and treated with Tongxinluo at different doses, and the proliferation and differentiation of the cells were observed by immunofluorescence staining at different time points.


RESULTS
The ratio of embryonic NSCs labeled with nestin decreased soon after Tongxinluo treatment, but increased afterwards. Significant difference was noted in the number of cells labeled with beta-tubulin between Tongxinluo group and the control group 3 and 7 days after the treatment, and also between high-dose and low-dose Tongxinluo groups at 7 days.


CONCLUSIONS
Tongxinluo can induce the proliferation and neuronal differentiation of rat embryonic NSCs, and the effect is related to the dose of Tongxinluo administered.
Jiang
Hai-shan
HS
Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. E-mail: jhsh1978@ yahoo.com.cn.
Li
Qiu-shi
QS
Lu
Bing-xun
BX
Wang
Li-xin
LX
Yin
Rui-xue
RX
D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D005455:Fluorescent Antibody Technique; D008297:Male; D009474:Neurons / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; C513941:tongxinluo