[Expression of OCTN2 mRNA in the human epididymis and its significance].
OBJECTIVE
To explore the mechanism of L-Carnitine transport and the expression of OCTN2 mRNA in the human epididymis so as to provide a theoretical basis for male contraception.


METHODS
We collected specimens from human epididymides and determined the expressions of OCTN2 mRNA in the caput, corpus and cauda of the epididymis by RT-PCR.


RESULTS
OCTN2 mRNA was expressed in the caput, corpus and cauda of the epididymis.


CONCLUSIONS
The human epididymis may rely on OCTN2 for transporting L-Carnitine into the epididymal duct to promote sperm maturation. With the accumulation of information on OCTN2 in the human epididymis, OCTN2 will become a new molecular target for researches on male contraception.
Gong
Dong-Ming
DM
Department of Urology, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200001, China. gongdongming@126.com
Li
Zheng
Z
Zhu
Xiao-Bin
XB
Liu
Yu-Lin
YL
Cao
Xiao-Rong
XR
Liu
Yong
Y
Wang
Yi-Xin
YX
D003267:Contraception / Q000379:methods; D004822:Epididymis / Q000378:metabolism*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D027701:Organic Cation Transport Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000074058:Solute Carrier Family 22 Member 5
D027701:Organic Cation Transport Proteins; D012333:RNA, Messenger; C492020:SLC22A5 protein, human; D000074058:Solute Carrier Family 22 Member 5