title: Bromadiolone poisoning: LC-MS method and pharmacokinetic data.
abstract: Poisoning with superwarfarins, like bromadiolone, is a growing public health problem, and the mortality is high. Pharmacokinetic data on bromadiolone in humans are however scarce, and there are no reports following repeated exposures to bromadiolone. We have developed a method for quantification of bromadiolone in whole blood, using liquid chromatography-mass spectrometry (LC-MS). The analytical method is reported. Limit of detection was 0.005 mg/L and limit of quantification was 0.01 mg/L. The concentrations of bromadiolone in whole blood and plasma in serial samples from a 62-year-old woman were measured. The half-life of bromadiolone in blood was estimated to be about 6 days in the initial phase of elimination and about 10-13 days in the terminal phase. The mean plasma/blood ratio of bromadiolone was 1.7 +/- 0.6. Stability testing of bromadiolone in whole blood samples after two cycles of freeze and thaw revealed that bromadiolone concentrations decreased.
authors.affiliation: Norwegian Institute of Public Health, Division of Forensic Toxicology and Drug Abuse, PO Box 4404, Nydalen, 0403 Oslo, Norway. vigdis.vindenes@fhi.no
authors.forename: Vigdis
authors.initials: V
authors.lastname: Vindenes
authors.forename: Ritva
authors.initials: R
authors.lastname: Karinen
authors.forename: Inger
authors.initials: I
authors.lastname: Hasvold
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Bernard
authors.forename: Jørg G
authors.initials: JG
authors.lastname: Mørland
authors.forename: Asbjørg S
authors.initials: AS
authors.lastname: Christophersen
mesh_terms: D015110:4-Hydroxycoumarins / Q000097:blood / Q000493:pharmacokinetics* / Q000506:poisoning*; D000925:Anticoagulants / Q000097:blood / Q000493:pharmacokinetics* / Q000506:poisoning*; D002853:Chromatography, Liquid; D004355:Drug Stability; D005260:Female; D053593:Forensic Toxicology; D006207:Half-Life; D006801:Humans; D013058:Mass Spectrometry; D008875:Middle Aged; D015394:Molecular Structure
chemical_list: D015110:4-Hydroxycoumarins; D000925:Anticoagulants; C021636:bromadiolone