title: Defective antigen presentation by monocytes in ESRD patients not responding to hepatitis B vaccination: impaired HBsAg internalization and expression of ICAM-1 and HLA-DR/Ia molecules.
abstract: This study was undertaken to evaluate the monocyte function of uraemic non-responders to hepatitis B vaccination. Therefore, some parameters concerning antigen processing by monocytes (Mo) as antigen presenting cells (APC) were analysed. It was found that in uraemic non-responders, (1) the internalization of HBsAg by monocytes was significantly decreasjed-HBsAg complexed with specific IgG or as immune complex isolated from patients is better internalized compared with free HBsAg; (2) during antigen presentation the expression of adhesion (ICAM-1) and accessory (HLA-DR/Ia) molecules was significantly decreased in uraemic patients, especially in non-responders; and (3) impaired internalization of HBsAg as well as a decrease in ICAM-1 and HLA-DR/Ia expression, correlated well with the blunted proliferation of CD4(+) T cells stimulated by autologous monocytes induced by HBsAg.
authors.affiliation: Department of Nephrology 5th Clinic of Internal Medicine University of Cologne Germany.
authors.forename: J
authors.initials: J
authors.lastname: Stachowski
authors.forename: C
authors.initials: C
authors.lastname: Barth
authors.forename: M
authors.initials: M
authors.lastname: Pollok
authors.forename: J
authors.initials: J
authors.lastname: Michałkiewicz
authors.forename: K
authors.initials: K
authors.lastname: Madaliński
authors.forename: J
authors.initials: J
authors.lastname: Maciejewski
authors.forename: C A
authors.initials: CA
authors.lastname: Baldamis