Molecular profiling of bladder cancer: involvement of the TGF-beta pathway in bladder cancer progression.
A human bladder cancer model of nine cell sublines derived from the BL17/2 cell line was used to evaluate genes related to disease progression. Molecular profiling of sublines that were non-tumorigenic and invasive in nude mice was performed and identified 1367 differentially-expressed genes. Quantitative real-time PCR analysis of six transforming growth factor-beta (TGF-beta) pathway genes using the entire panel of nine cell lines was performed. Bone morphogenetic protein-2 expression was significantly associated with in vivo tumorigenicity of the cell lines (p=0.0228, Mann-Whitney); inhibin-betaB was related to their invasiveness (p=0.0468, Mann-Whitney). Analysis of conditioned medium showed TGF-beta1 production to be significantly associated with the phenotype of the cell line. The study shows the possible involvement of the TGF-beta pathway in bladder cancer progression.
Hung
Tzong-Tyng
TT
Oncology Research Centre, Level 2, Clinical Sciences Building, Prince of Wales Hospital, Barker Street, Randwick, NSW 2031, Australia.
Wang
Hong
H
Kingsley
Elizabeth A
EA
Risbridger
Gail P
GP
Russell
Pamela J
PJ
D045744:Cell Line, Tumor; D020869:Gene Expression Profiling; D006801:Humans; D015398:Signal Transduction; D016212:Transforming Growth Factor beta / Q000502:physiology*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism* / Q000473:pathology
D016212:Transforming Growth Factor beta