An in vitro comparative study of follicle stimulating hormone (FSH) and activin A effects on the maturation of preantral follicle-enclosed oocytes from immature Syrian mice.
It was aimed to investigate the effects of different doses of follicle stimulating hormone (FSH) and activin A on the growth and maturation of preantral mouse follicles during the in vitro culture.



Preantral follicles (90-100 microm in diameter) were harvested from 6-8 week-old Syrian mice and cultured in TCM199 culture medium for 6 days to see the effect of FSH and Activin A. Activin A concentrations in the range of 10-200 ng/ml were used, while 10, 50, 100, 150 and 200 mIU/ml FSH were used in the experiment.



Activin A concentration of 100 ng/ml resulted in a significant increase in follicle diameter (170 microm) with the survival rate of 73% as compared to the control (100 microm and 25%, P<0.05). The number of oocytes matured and the percentage of germinal vesicle breakdown (GVBD) was 61 and 70%, respectively as compared to the control (20 and 29%, P<0.05). Follicle diameter (190 microm) and survival rate (85%) increased significantly in the presence of 100 mIU/ml of FSH as compared to the control (P<0.05). But, the administration of activin A+FSH increased the effect of both factors on follicular diameter (205 microm as compared to 100 microm in control, P<0.01). Follicle survival, oocyte maturation and GVBD rates were 91, 81 and 89%, respectively (P<0.01).



These results have suggested that exposure to FSH and activin A before the formation of antral cavity had positive effect on follicle survival and oocyte robustness.
Javed
Aisha
A
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.|Molecular Biochemistry Lab, Dept.  of Biochemistry, University of Agriculture, Faisalabad, Pakistan.
Jamil
Amer
A
Molecular Biochemistry Lab, Dept.  of Biochemistry, University of Agriculture, Faisalabad, Pakistan.
Rezaei-Zarchi
Saeed
S
Dept. of Biology, Payam-e-Noor University of Taft, Yazd, Iran.
Kalantar
Seyed Mehdi
SM
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.|Yazd Medical Biotechnology and Genetic Engineering Incubator, Yazd, Iran.
Anvari
Morteza
M
Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Nazem
Habib
H
Central Payam-e-Noor University, Tehran, Iran.
D028341:Activins / Q000502:physiology*; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004357:Drug Synergism; D005260:Female; D043373:Follicle Stimulating Hormone, Human / Q000494:pharmacology* / Q000502:physiology; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D009866:Oogenesis / Q000502:physiology; D006080:Ovarian Follicle / Q000187:drug effects / Q000254:growth & development* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000494:pharmacology; D046509:Tissue Culture Techniques
D043373:Follicle Stimulating Hormone, Human; D011994:Recombinant Proteins; C422405:activin A; D028341:Activins