title: Determination of phytase activity in feed: interlaboratory study.
abstract: An interlaboratory study was conducted to determine the performance characteristics of a new method for the determination of phytase activity in feed samples. The method is based on the principle that inorganic phosphate is released from the substrate phytate under defined assay conditions and has been validated for its suitability to measure the enzyme activity of various phytase products. Two different experimental designs of the study were applied, allowing for the estimation of the precision of the method under repeatability, intermediate precision and reproducibility conditions, respectively. The relative standard deviation for repeatability (RSDr) ranged from 2.2 to 10.6% and the RSD for reproducibility (RSDR) ranged from 5.4 to 15%. The suitability of the validated method for the intended purpose was demonstrated. The obtained performance profile of the method validated in this study was comparable to that of similar methods that were exclusively validated for one phytase product.
authors.affiliation: European Commission, Joint Research Centre, Institute for Reference Materials and Measurements, Retieseweg 111, B-2440 Geel, Belgium.
authors.forename: Gisele
authors.initials: G
authors.lastname: Gizzi
authors.forename: Peter
authors.initials: P
authors.lastname: Thyregod
authors.forename: Christoph
authors.initials: C
authors.lastname: von Holst
authors.forename: Gerard
authors.initials: G
authors.lastname: Bertin
authors.forename: Kurt
authors.initials: K
authors.lastname: Vogel
authors.forename: Mai
authors.initials: M
authors.lastname: Faurschou-Isaksen
authors.forename: Roland
authors.initials: R
authors.lastname: Betz
authors.forename: Richard
authors.initials: R
authors.lastname: Murphy
authors.forename: Betina Brandt
authors.initials: BB
authors.lastname: Andersen
mesh_terms: D010832:6-Phytase / Q000378:metabolism*; D000821:Animal Feed / Q000032:analysis*
chemical_list: D010832:6-Phytase