Covalent surface modification of titanium oxide with different adhesive peptides: surface characterization and osteoblast-like cell adhesion.
A fundamental goal in the field of implantology is the design of innovative devices suitable for promoting implant-to-tissue integration. This result can be achieved by means of surface modifications aimed at optimizing tissue regeneration. In the framework of oral and orthopedic implantology, surface modifications concern both the optimization of titanium/titanium alloy surface roughness and the attachment of biochemical factors able to guide cellular adhesion and/or growth. This article focuses on the covalent attachment of two different adhesive peptides to rough titanium disks. The capability of biomimetic surfaces to increase osteoblast adhesion and the specificity of their biological activity due to the presence of cell adhesion signal-motif have also been investigated. In addition, surface analyses by profilometry, X-ray photoelectron spectroscopy, and time of flight-secondary ion mass spectrometry have been carried out to investigate the effects and modifications induced by grafting procedures.
Dettin
Monica
M
Department of Chemical Process Engineering, University of Padova, via Marzolo 9, 35131 Padova, Italy. monica.dettin@unipd.it
Bagno
Andrea
A
Gambaretto
Roberta
R
Iucci
Giovanna
G
Conconi
Maria Teresa
MT
Tuccitto
Nunzio
N
Menti
Anna Michela
AM
Grandi
Claudio
C
Di Bello
Carlo
C
Licciardello
Antonino
A
Polzonetti
Giovanni
G
D000595:Amino Acid Sequence; D000818:Animals; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology / Q000378:metabolism; D005260:Female; D006801:Humans; D008422:Materials Testing; D008969:Molecular Sequence Data; D015394:Molecular Structure; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012821:Silanes / Q000737:chemistry; D018629:Spectrometry, Mass, Secondary Ion; D013499:Surface Properties; D014025:Titanium / Q000737:chemistry / Q000378:metabolism
D020099:Coated Materials, Biocompatible; D010455:Peptides; D012821:Silanes; C009495:titanium dioxide; D014025:Titanium