title: Acanthamoeba castellanii: identification and distribution of actin cytoskeleton.
abstract: The presence of the cytoskeleton of Acanthamoeba castellanii was observed by means of cryo-electronmicroscopy and immunofluorescence techniques. This structure is formed largely by fibers and networks of actin located mainly in cytoplasmic locomotion structures as lamellipodia and as well as in various endocytic structures. In addition, the comparison between total actin content in whole extracts among different amoebae was made. The molecular weight of actin in A. castellanii was 44 kDa, and 45 kDa for Naegleria fowleri and Entamoeba histolytica.
authors.affiliation: Department of Experimental Pathology, Center for Research and Advanced Studies, Mexico City, Mexico. goroa@cinvestav.mx
authors.forename: Arturo
authors.initials: A
authors.lastname: González-Robles
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Castañón
authors.forename: Verónica Ivonne
authors.initials: VI
authors.lastname: Hernández-Ramírez
authors.forename: Lizbeth
authors.initials: L
authors.lastname: Salazar-Villatoro
authors.forename: Mónica
authors.initials: M
authors.lastname: González-Lázaro
authors.forename: Maritza
authors.initials: M
authors.lastname: Omaña-Molina
authors.forename: Patricia
authors.initials: P
authors.lastname: Talamás-Rohana
authors.forename: Adolfo
authors.initials: A
authors.lastname: Martínez-Palomo
mesh_terms: D015823:Acanthamoeba Keratitis / Q000469:parasitology; D048248:Acanthamoeba castellanii / Q000737:chemistry / Q000648:ultrastructure*; D000199:Actins / Q000032:analysis*; D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D015925:Cryopreservation; D003599:Cytoskeleton / Q000737:chemistry / Q000648:ultrastructure*; D004285:Dogs; D004591:Electrophoresis, Polyacrylamide Gel; D004748:Entamoeba histolytica / Q000737:chemistry / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D016848:Naegleria fowleri / Q000737:chemistry / Q000648:ultrastructure
chemical_list: D000199:Actins