[Immunoenzimatic detection of the Clostridium tetani bacterial toxin: an alternative to mice bioassays].
Cell-free extracts from 20 strains of Clostridium tetani isolated from soil samples, were tested for tetanus toxin production using an enzyme immunoassay. All the extracts were classified as positive for the toxin presence, and eight of them showed absorbance values corresponding to tetanus toxin concentrations between 3.2 and 88 ng/ml; thus, they fell within the linear absorbance range (0.135-0.317). All dilutions of toxin used to obtain the calibration curve (0.0071 to 1.1 ng) were lethal for mice.
Chaves
Fernando
F
Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. fchaves@icp.ucr.ac.cr
León
Guillermo
G
Hernández-Chavarría
Francisco
F
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000097:blood*; D001681:Biological Assay; D003017:Clostridium tetani / Q000737:chemistry*; D004195:Disease Models, Animal; D005188:False Negative Reactions; D007124:Immunoenzyme Techniques / Q000379:methods*; D051379:Mice; D011817:Rabbits; D012756:Sheep; D012988:Soil Microbiology; D013742:Tetanus / Q000209:etiology / Q000517:prevention & control; D013744:Tetanus Toxin / Q000032:analysis* / Q000096:biosynthesis / Q000633:toxicity
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D013744:Tetanus Toxin