Elicitation effects of oligosaccharides on the transcriptional level of bacitracin ABC transporter genes in Bacillus licheniformis.
Bacitracin ABC transporter comprises a self-resistance system in Bacillus licheniformis against its own product (bacitracin). Elicitation effects of carbohydrates on transcription level of bcrABC genes encoding bacitracin ABC transporter system were investigated through quantification by real-time PCR. Increases of 68% and 29% in bacitracin A yields after 36 h were achieved in cultures supplemented with oligoguluronate and oligoguluronate/mannan oligosaccharide combination. Cultures with combined elicitors showed 3, 2.6 and 3 transcriptional fold-changes in bcrA, bcrB and bcrC, respectively, compared to control. This could be due to a secondary effect caused by increased bacitracin A yield through elicitation.
Murphy
Tania
T
Applied Biotechnology Research Group, Department of Molecular & Applied Biosciences, University of Westminster, London, UK.
Roy
Ipsita
I
Harrop
Anthony
A
Dixon
Keith
K
Keshavarz
Tajalli
T
D018528:ATP-Binding Cassette Transporters / Q000235:genetics*; D001407:Bacillus / Q000187:drug effects* / Q000235:genetics*; D001414:Bacitracin / Q000378:metabolism*; D002138:Calibration; D004351:Drug Resistance / Q000235:genetics; D018628:Gene Dosage; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D005798:Genes, Bacterial; D009844:Oligosaccharides / Q000494:pharmacology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic / Q000187:drug effects*
D018528:ATP-Binding Cassette Transporters; D009844:Oligosaccharides; D001414:Bacitracin