Inactivated Salmonella expressing the receptor-binding domain of bacterial adhesins elicit antibodies inhibiting hemagglutination.
We examined the potential of inactivated Salmonella strains to induce protective antibodies against two adhesins of pathogenic Escherichia coli. The receptor-binding domains of the F17a-G adhesin of F17a fimbriae and of the FimH adhesin of type 1 fimbriae were fused to the translocator domain of the autotransporter AIDA-I. An IgG response against F17a-G or FimH was induced after immunization of mice with acetone-inactivated Salmonella displaying the corresponding fimbrial receptor-binding domain. These sera inhibit in vitro agglutination of erythrocytes by E. coli carrying these fimbriae. Our results demonstrate that induced and subsequently acetone-inactivated Salmonella are useful delivery vehicles for the stimulation of an IgG antibody response against heterologous antigens.
Van Gerven
Nani
N
Onderzoeksgroep Genetische Virologie, Vrije Universiteit Brussel, B-1050 Brussels, Belgium. Nani.Van.Gerven@vub.ac.be
De Greve
Henri
H
Hernalsteens
Jean-Pierre
JP
D018829:Adhesins, Bacterial / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000907:Antibodies, Bacterial; D004912:Erythrocytes / Q000276:immunology; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D006384:Hemagglutination / Q000276:immunology*; D051379:Mice; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins; D012475:Salmonella / Q000235:genetics / Q000378:metabolism*
D018829:Adhesins, Bacterial; D000907:Antibodies, Bacterial; D029968:Escherichia coli Proteins; D011994:Recombinant Proteins