[The mRNA expression of P16(ink4a) and HST2 in benign prostatic hyperplasia tissues: a pilot study].
OBJECTIVE
To investigate the expressions of the aging gene P16(ink4a) and anti-aging gene HST2 in benign prostatic hyperplasia (BPH).


METHODS
Twenty-three BPH and eighteen normal prostate specimens were collected and total RNA was extracted, followed by the reverse transcriptase polymerase chain reaction (RT-PCR). The expressions of P16(ink4a) was detected by semi-quantitative analysis in BPH and normal prostate tissues.


RESULTS
P16(ink4a) mRNA, rather than HST2, was expressed in the BPH and normal prostate tissues. Semi-quantitative analysis showed that the P16(ink4a) mRNA expression in the normal prostate tissues (0.4868 +/- 0.545 was significantly higher than in the BPH tissues (0.2783 +/- 0.0268, with a statistical difference in between (P < 0. 05).


CONCLUSIONS
P16(ink4a) might play an important role in the pathogenesis of BPH and is probably one of the factors of cell aging escape.
Xiong
Ying
Y
Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Pan
Tie-Jun
TJ
Ye
Zhang-Qun
ZQ
D000328:Adult; D000368:Aged; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000235:genetics*; D051522:Fibroblast Growth Factor 6 / Q000235:genetics*; D020869:Gene Expression Profiling; D006801:Humans; D008297:Male; D010865:Pilot Projects; D011470:Prostatic Hyperplasia / Q000235:genetics / Q000473:pathology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D019941:Cyclin-Dependent Kinase Inhibitor p16; C496452:FGF6 protein, human; D051522:Fibroblast Growth Factor 6; D012333:RNA, Messenger