A modified mini-primer set for analyzing mitochondrial DNA control region sequences from highly degraded forensic samples.
To facilitate the analysis of mitochondrial DNA (mtDNA) control region sequences from highly degraded skeletal remains, a modified mini-primer set was designed to overcome the limitations of the Armed Forces DNA Identification Laboratory (AFDIL) mini-primer set. This modified mini-primer set is less affected by nucleotide variability and PCR amplification conditions than the AFDIL mini-primer set, and was able to amplify the mtDNA sequences of 55-year-old skeletal remains with high efficiency, indicating that it is a useful tool for analyzing mtDNA control region sequences from highly degraded forensic samples.
Lee
Hwan Young
HY
Department of Forensic Medicine, Yonsei University College of Medicine, Yonsei University, Seoul, Korea.
Kim
Na Young
NY
Park
Myung Jin
MJ
Yang
Woo Ick
WI
Shin
Kyoung-Jin
KJ
D017931:DNA Primers; D004272:DNA, Mitochondrial / Q000032:analysis* / Q000737:chemistry; D018732:Forensic Anthropology / Q000379:methods*; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013997:Time Factors
D017931:DNA Primers; D004272:DNA, Mitochondrial