Zinc oxide nanoparticles-chitosan composite film for cholesterol biosensor.
Zinc oxide nanoparticles (NanoZnO) uniformly dispersed in chitosan (CHIT) have been used to fabricate a hybrid nanocomposite film onto indium-tin-oxide (ITO) glass plate. Cholesterol oxidase (ChOx) has been immobilized onto this NanoZnO-CHIT composite film using physiosorption technique. Both NanoZnO-CHIT/ITO electrode and ChOx/NanoZnO-CHIT/ITO bioelectrode have been characterized using Fourier transform-infrared (FTIR), X-ray diffraction (XRD), cyclic voltammetry (CV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) techniques, respectively. The ChOx/NanoZnO-CHIT/ITO bioelectrode exhibits linearity from 5 to 300 mg dl(-1) of cholesterol with detection limit as 5 mg dl(-1), sensitivity as 1.41x10(-4) A mg dl(-1) and the value of Michaelis-Menten constant (K(m)) as 8.63 mg dl(-1). This cholesterol biosensor can be used to estimate cholesterol in serum samples.
Khan
Raju
R
Biomolecular Electronics & Conducting Polymer Research Group, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi 110 012, India.
Kaushik
Ajeet
A
Solanki
Pratima R
PR
Ansari
Anees A
AA
Pandey
Manoj K
MK
Malhotra
B D
BD
D015374:Biosensing Techniques; D048271:Chitosan / Q000737:chemistry*; D002784:Cholesterol / Q000032:analysis*; D002789:Cholesterol Oxidase / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry; D006801:Humans; D007204:Indium / Q000737:chemistry; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning / Q000379:methods; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry; D010316:Particle Size; D010783:Photometry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods; D014001:Tin / Q000737:chemistry; D014961:X-Ray Diffraction; D015034:Zinc Oxide / Q000737:chemistry*
D004800:Enzymes, Immobilized; D008567:Membranes, Artificial; D010087:Oxides; D007204:Indium; D014001:Tin; D048271:Chitosan; D002784:Cholesterol; D002789:Cholesterol Oxidase; D015034:Zinc Oxide