[Correlation analysis of microsatellite DNA markers with body weight, length and height of common carp (Cyprinus carpio L.)].
Forty-seven microsatellite markers were selected to analyze the genomic DNA of 92 progenies derived from the recombinant inbred lines (RIL) of common carp, which came from the cross between Barbless carp and Hebao-cold tolerance red carp. The results showed that a total of 162 different alleles were found, and the number of alleles in each locus was 2 to 6. The DNA fragment length was 100 bp to 444 bp, and the number of mean valid alleles was 1.3069 to 4.2288. The value of heterozygosity was 0.2361 to 0.7677, and the mean polymorphism information content (PIC) was 0.5368. A GLM procedure was used to analyze the effects of these 47 microsatellites on body weight, length and height. Results uncovered HLJ695, HLJ716, HLJ739, HLJ759, HLJ774 and K16 had a significant impact on body weight, length and height, and HLJ776 had a significant impact on height. In addition, the genotypes of these correlative loci were determined.
Zhang
Yi-Feng
YF
Heilongjiang Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin 150070, China. feng_1027@126.com
Zhang
Yan
Y
Lu
Cui-Yun
CY
Cao
Ding-Chen
DC
Sun
Xiao-Wen
XW
D000818:Animals; D049628:Body Size / Q000235:genetics*; D001835:Body Weight / Q000235:genetics*; D002347:Carps / Q000235:genetics* / Q000254:growth & development; D005838:Genotype; D018895:Microsatellite Repeats / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics