Live birth after vitrification of in vitro matured human oocytes.
OBJECTIVE
To report the first healthy live birth from immature oocytes retrieved in a natural menstrual cycle, followed by in vitro maturation (IVM) and cryopreservation of the oocytes by vitrification.


METHODS
Case report.


METHODS
University-based tertiary medical center.


METHODS
A 27-year-old woman with tubal disease and polycystic ovaries.


METHODS
Immature oocytes were retrieved by transvaginal ultrasound guided follicle aspiration on day 13 of her natural menstrual cycle, matured in vitro and vitrified. The oocytes were thawed in a subsequent menstrual cycle, inseminated by intracytoplasmic sperm injection, and the resulting embryos transferred.


METHODS
Oocyte maturation and survival rates, pregnancy, and live birth.


RESULTS
One metaphase II and 18 germinal vesicle stage oocytes were collected; 16 out of 18 germinal vesicle oocytes matured, and a total of 17 oocytes were vitrified. After thawing, four IVM oocytes survived; three embryos were transferred. The woman went on to deliver a single healthy live baby at term.


CONCLUSIONS
We provide proof-of-principle evidence that the novel fertility preservation strategy of immature oocyte retrieval, IVM, and vitrification of oocytes can lead to successful pregnancy and healthy live birth.
Chian
Ri-Cheng
RC
McGill Reproductive Centre, McGill University Health Centre, McGill University, Montreal, Quebec, Canada.
Gilbert
Lucy
L
Huang
Jack Y J
JY
Demirtas
Ezgi
E
Holzer
Hananel
H
Benjamin
Alice
A
Buckett
William M
WM
Tulandi
Togas
T
Tan
Seang Lin
SL
D000328:Adult; D018929:Cell Culture Techniques; D015925:Cryopreservation; D010064:Embryo Implantation; D004624:Embryo Transfer; D005184:Fallopian Tube Diseases / Q000150:complications* / Q000628:therapy; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007247:Infertility, Female / Q000209:etiology / Q000628:therapy*; D050498:Live Birth; D054315:Oocyte Retrieval; D009865:Oocytes; D011085:Polycystic Ovary Syndrome / Q000150:complications* / Q000628:therapy; D011247:Pregnancy; D020554:Sperm Injections, Intracytoplasmic; D016896:Treatment Outcome; D018084:Ultrasonography, Interventional