Effects of 16 pure hydrocarbons and two oils on haemocyte and haemolymphatic parameters in the Pacific oyster, Crassostrea gigas (Thunberg).
The in vitro effects of polycyclic aromatic hydrocarbons (PAHs) on haemocyte and haemolymphatic parameters of the Pacific oyster, Crassostrea gigas, were tested using field concentrations (10(-7) and 10(-9) mg mL(-1)) observed in the Marennes-Oleron Basin (Charente-Maritime, France) and high concentrations (10(-3) and 10(-5) mg mL(-1)) observed during oil spills. As a first step, the effects of pollutants, after a 24 h contamination period, were monitored on individual and pooled haemolymph samples and similar results were observed for both sample types. In a second step, haemolymphs from 45 oysters were withdrawn and pooled. Eighteen pollutants were tested in vitro after a 4 and 24h contamination period and 10 of them showed significant modulations of the five haemocyte parameters tested. Seven pollutants (fluorene, pyrene, anthracene, phenanthrene, chrysene, indeno[1,2,3-c,d]pyrene and heavy fuel oil (HFO)) induced a decrease in haemocyte mortality. Fluorene, pyrene and HFO significantly decreased phagocytosis activity. Percentage of non-specific esterase positive cells, phenoloxidase activity and lysosome presence were increased by naphthalene, benzo[b]fluoranthene and dibenz[a,h]anthracene, respectively. Modulation of immune parameters in the Pacific oyster by PAHs suggested that PAH pollution may be related to enhanced susceptibility to diseases.
LIENSs/Littoral ENvironnement et Sociétés, UMR 6250 CNRS, Université de La Rochelle, 2 rue Olympe de Gouges, La Rochelle, France. Anne.Bado.Nilles@cedre.fr
Anne
A
Bado-Nilles
Béatrice
B
Gagnaire
Hélène
H
Thomas-Guyon
Stéphane
S
Le Floch
Tristan
T
Renault
D000818:Animals; D049896:Crassostrea / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006434:Hemocytes / Q000187:drug effects / Q000378:metabolism; D006458:Hemolymph / Q000187:drug effects / Q000378:metabolism; D009821:Oils / Q000008:administration & dosage / Q000633:toxicity*; D010587:Phagocytosis / Q000187:drug effects; D011084:Polycyclic Aromatic Hydrocarbons / Q000008:administration & dosage / Q000633:toxicity*; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
D009821:Oils; D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical