Subproteomic analysis of the mitochondrial proteins in rats 24 h after partial hepatectomy.
A 70% partial hepatectomy (70%PHx) induces cell proliferation until the original mass of the liver is restored. Mitochondria are involved directly in the process of liver regeneration (LR); however, their role in the early phase of LR is not clear. In an attempt to identify mitochondrial proteins that are correlated with the early phase of LR, we obtained a mitochondrial fraction via Nycodenz(R) density gradient centrifugation and subcellular proteomic analysis was performed. The mitochondrial proteins were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Compared to the sham-operation control group, 3 proteins were up-regulated and 22 proteins were down-regulated at 24 h after 70%PHx. We identified 22 differentially expressed proteins that were associated with carbohydrate metabolism, lipid metabolism, the respiratory chain and oxidation-phosphorylation, biotransformation and other metabolic pathways. Prohibitin, a proliferation-regulating protein that was down-regulated at 24 h after PHx, was analyzed by applying RNAi (PHBi) with BRL-3A. This demonstrated a decreased mitochondrial membrane potential, implying a potential role in maintaining mitochondrial integrity. These results indicated that hepatic mitochondrial adaptations to LR after 70%PHx affect various cellular metabolic pathways, which advances our knowledge of the role of mitochondria in the early phase of LR.
Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai 200433, China.
Qingju
Q
Sun
Mingyong
M
Miao
Xuan
X
Jia
Wei
W
Guo
Lianghua
L
Wang
Zhenzhen
Z
Yao
Chunli
C
Liu
Binghua
B
Jiao
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D006498:Hepatectomy; D008297:Male; D053078:Membrane Potential, Mitochondrial; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D040901:Proteomics; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D024101:Mitochondrial Proteins; D034741:RNA, Small Interfering