Detection of a lysozyme inhibitor in Proteus mirabilis by a new reverse zymogram method.
A reverse zymogram method for the detection of bacterial lysozyme inhibitors was developed. This method was validated by using a periplasmic protein extract of Escherichia coli containing a known inhibitor and subsequently led to the detection of a new proteinaceous hen egg white lysozyme inhibitor in Proteus mirabilis.
Laboratory of Food Microbiology and Leuven Food Science and Nutrition Research Center (LFoRCe), Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Leuven, Belgium.
Lien
L
Callewaert
Lise
L
Vanderkelen
Daphne
D
Deckers
Abram
A
Aertsen
Johan
J
Robben
Chris W
CW
Michiels
D000595:Amino Acid Sequence; D004791:Enzyme Inhibitors / Q000302:isolation & purification*; D004792:Enzyme Precursors / Q000737:chemistry / Q000235:genetics; D008969:Molecular Sequence Data; D009113:Muramidase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000235:genetics*; D010047:Ovalbumin / Q000378:metabolism; D011513:Proteus mirabilis / Q000737:chemistry* / Q000201:enzymology; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D004791:Enzyme Inhibitors; D004792:Enzyme Precursors; D010047:Ovalbumin; D009113:Muramidase