Effects of a static magnetic field on audiogenic seizures in black Swiss mice.
Effects of a static magnetic field (SMF) with strong gradient components were studied in black Swiss mice. Exposure to SMF (100-220 mT, 15-40 T/m for 1h) did not affect the threshold for detecting auditory brainstem responses. Serial seizures elevated the hearing threshold at some frequencies, but there was no difference between SMF-exposed and unexposed control mice. EEG changes were recorded during audiogenic seizures. Pretreatment with SMF prolonged seizure latency in response to stimulation with white noise of increasing intensity from 74 to 102 dBA (1 min interval between 2 and 4 dBA increments) without significant effects on seizure severity. Gender-related differences were not statistically significant. Stimulation with 10 min sound steps revealed prolongation of latency and reduction of seizure severity in SMF-exposed, but not unexposed, mice. Pretreatment with phenytoin (5 mg/kg) in combination with SMF had significantly greater effects on seizure latency and severity than either pretreatment alone. These findings indicate that the SMF studied here under different conditions elevated seizure threshold and had anticonvulsant properties in Black Swiss mice and increased the efficacy of a conventional anticonvulsant drug.
Department of Neurology, Vanderbilt University Medical Center, Nashville, TN 37212, USA. michael.mclean@vanderbilt.edu
Michael J
MJ
McLean
Stefan
S
Engström
Zhang
Z
Qinkun
Christopher
C
Spankovich
Daniel B
DB
Polley
Daniel
D
Polley
D000161:Acoustic Stimulation / Q000379:methods; D000818:Animals; D000831:Animals, Newborn; D000927:Anticonvulsants / Q000627:therapeutic use; D001522:Behavior, Animal / Q000528:radiation effects; D004195:Disease Models, Animal; D004307:Dose-Response Relationship, Radiation; D004569:Electroencephalography; D004574:Electromagnetic Fields; D020195:Epilepsy, Reflex / Q000503:physiopathology* / Q000628:therapy*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000187:drug effects / Q000528:radiation effects*; D051379:Mice; D010672:Phenytoin / Q000627:therapeutic use; D011571:Psychoacoustics; D011930:Reaction Time / Q000187:drug effects / Q000528:radiation effects; D012720:Severity of Illness Index
D000927:Anticonvulsants; D010672:Phenytoin