HSV-1 ICP27 suppresses NF-kappaB activity by stabilizing IkappaBalpha.
Nuclear factor kappaB (NF-kappaB) is associated with the transcriptional activation of genes encoding chemokines, adhesion molecules, cytokines, and anti-apoptotic proteins, which are key components in immune responses and viral infection. Many viruses modulate NF-kappaB through numerous viral gene products to allow productive infections and immune escape. Here we report that herpes simplex virus-1 infected cell protein 27 (HSV-1 ICP27), an immediate early protein of HSV-1, represses NF-kappaB activity through binding to inhibitor of kappaB (IkappaBalpha), blocking phosphorylation and ubiquitination of IkappaBalpha, and stabilizing IkappaBalpha. These data may explain how NF-kappaB activity is regulated by ICP27 to escape immune responses during the very early period of HSV-1 infection.
Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
Jin Chul
JC
Kim
Soo Yun
SY
Lee
Sang Young
SY
Kim
Jeong Ki
JK
Kim
Hye Jin
HJ
Kim
Hee Min
HM
Lee
Mi Sun
MS
Choi
Jung Sun
JS
Min
Mi Jee
MJ
Kim
Hyang Soon
HS
Choi
Jeong Keun
JK
Ahn
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D020672:I-kappa B Proteins / Q000378:metabolism*; D017874:Immediate-Early Proteins / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010766:Phosphorylation; D054875:Ubiquitination; D014709:Vero Cells
D020672:I-kappa B Proteins; C057120:ICP27 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha