Ionic liquids and proteases: a clean alliance for semisynthesis.
Herein we present the first report on protease-catalysed ligation of cleavage-sensitive peptide and protein fragments in ionic-liquid-containing solvent systems. By applying the newly established [MMIM][Me2PO4]/buffer mixture as a reaction medium, significant advantages over purely aqueous or conventional organic solvent-containing media could be identified, including in particular the use of active wild-type proteases as biocatalysts, the suppression of any competitive proteolytic side reactions, the high turnover rates compared to classical organic solvents and the high stability of chemically labile reactants.
Wehofsky
Nicole
N
Max Planck Research Unit for Enzymology of Protein Folding, Weinbergweg 22, 06120 Halle/Saale, Germany.
Wespe
Christian
C
Cerovsky
Vaclav
V
Pech
Andreas
A
Hoess
Eva
E
Rudolph
Rainer
R
Bordusa
Frank
F
D000595:Amino Acid Sequence; D000818:Animals; D002021:Buffers; D002384:Catalysis; D002417:Cattle; D006868:Hydrolysis / Q000187:drug effects; D052578:Ionic Liquids / Q000737:chemistry* / Q000494:pharmacology; D008969:Molecular Sequence Data; D010755:Organophosphates / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism*; D010447:Peptide Hydrolases / Q000378:metabolism*; D012995:Solubility; D012997:Solvents / Q000737:chemistry; D013379:Substrate Specificity
D002021:Buffers; D052578:Ionic Liquids; D010755:Organophosphates; D010446:Peptide Fragments; D012997:Solvents; D010447:Peptide Hydrolases