L-Ribulose production from L-arabinose by an L-arabinose isomerase mutant from Geobacillus thermodenitrificans.
Geobacillus thermodenitrificans, with a double-site mutation in L-arabinose isomerase, produced 95 g L-ribulose l(-1 ) from 500 g L-arabinose l(-1) under optimum conditions of pH 8, 70 degrees C, and 10 units enzyme ml(-1) with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75 degrees C were 35 and 4.5 h, respectively.
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea.
Soo-Jin
SJ
Yeom
Jung-Hwan
JH
Ji
Ran-Young
RY
Yoon
Deok-Kun
DK
Oh
D019747:Aldose-Ketose Isomerases / Q000235:genetics / Q000378:metabolism*; D001089:Arabinose / Q000378:metabolism*; D004795:Enzyme Stability; D045302:Geobacter / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D009154:Mutation; D010429:Pentoses / Q000096:biosynthesis*; D013696:Temperature
D010429:Pentoses; C100182:ribulose; D001089:Arabinose; D019747:Aldose-Ketose Isomerases; C019715:L-arabinose isomerase