Composition and proposed structure of a water-soluble glycan from the Keumsa Sangwhang Mushroom (Phellinus linteus).
Phellinus linteus is a mushroom which has been prized in Korea for its medicinal properties since ancient times. A glycan preparation from this source has shown promise in experimental anti-tumour and metastasis studies, but the glycan has not been well characterized. In the present work, the glycan fraction of a hot water extract from P. linteus (Keumsa Sangwhang Mushroom, Korea) has been isolated following ammonium sulfate precipitation, dialysis/concentration and anion exchange chromatographic steps. Analyses for monosaccharide composition showed glucose and mannose to be the major constituents. Digestion of the glycan fraction with specific glycosidases and by linkage analysis allowed us to propose a core beta(1-3) linked glucan heavily substituted via (1-6) links with beta(1-3) linked mannose chains. Significant levels of galactose and xylose, present in the glycan fraction, may be associated with this glucomannan or not. These findings are consistent with the view that a core beta(1-3)-linked glucan chain with beta(1-6) branch points is a common feature of mushroom glycans possessing anti-tumour activity.
Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, Alabama, United States. johnb@uab.edu
John R
JR
Baker
Jeong-Su
JS
Kim
Soon-Young
SY
Park
D000363:Agaricales / Q000737:chemistry*; D002236:Carbohydrate Conformation; D007723:Korea; D010946:Plants, Medicinal / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D011134:Polysaccharides; D014867:Water