Autoimmune dacryoadenitis and keratoconjunctivitis induced in rabbits by subcutaneous injection of autologous lymphocytes activated ex vivo against lacrimal antigens.
Autologous peripheral blood lymphocytes (PBL), activated in a mixed cell reaction when co-cultured with purified rabbit lacrimal epithelial cells, are known to induce a Sjögren's-like autoimmune dacryoadenitis and keratoconjunctivitis when injected directly back into the donor animal's inferior lacrimal gland (LG). This study shows that autoreactive lymphocytes injected subcutaneously in a site away from the LG is capable of inducing an autoimmune disease in a rabbit. Induced disease (ID) develops more slowly, taking 4weeks as compared to 2weeks in the direct injection model. Initially, both clinical symptoms and histopathology are less pronounced than in the direct injection ID model, but later the immunocytochemistry shows the same CD4+/CD8+ ratio of 4:1 for both injection methods. The finding that lymphocytes activated against lacrimal antigens can travel or home from the injection site back to the inferior and superior LG, as well as the conjunctiva, suggests that these anatomical sites may have common epitopes that induce pathogenic CD4+ T cells that produce a Sjögren's-like syndrome.
Ocular Surface Center, Department. of Ophthalmology, Doheny Eye Institute, 1450 San Pablo Street, Los Angeles, CA 90033-4682, USA.
P B
PB
Thomas
Z
Z
Zhu
S
S
Selvam
D M
DM
Samant
D
D
Stevenson
A K
AK
Mircheff
J E
JE
Schechter
S W
SW
Song
M D
MD
Trousdale
D000818:Animals; D000954:Antigens, Surface / Q000276:immunology; D001324:Autoantigens / Q000276:immunology*; D001327:Autoimmune Diseases / Q000276:immunology* / Q000473:pathology; D003607:Dacryocystitis / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000276:immunology; D005260:Female; D007279:Injections, Subcutaneous; D007637:Keratoconjunctivitis / Q000276:immunology* / Q000473:pathology; D007765:Lacrimal Apparatus / Q000276:immunology* / Q000473:pathology; D008213:Lymphocyte Activation / Q000276:immunology; D017710:Lymphocyte Transfusion; D008214:Lymphocytes / Q000276:immunology* / Q000473:pathology; D011817:Rabbits; D014182:Transplantation, Autologous
D000954:Antigens, Surface; D001324:Autoantigens