Evaluation of microvascular endothelial function in patients with type 1 diabetes using laser-Doppler perfusion monitoring: which method to choose?
The evaluation of microvascular function is essential in the investigation of the pathophysiology of cardiometabolic diseases [Struijker-Boudier, H.A. et al., 2007. Evaluation of the microcirculation in hypertension and cardiovascular disease. Eur. Heart J. 28, 2834-2840]. In clinical research and practice, the study of microcirculation is of great value in the assessment of the effects of medical interventions and monitoring disease progression. It is well-known that patients with type 1 and 2 diabetes have microvascular dysfunction that results from numerous factors including hyperglycemia, oxidative stress and insulin resistance [Schalkwijk, C.G., Stehouwer, C.D., 2005. Vascular complications in diabetes mellitus: the role of endothelial dysfunction. Clin. Sci. (Lond). 109, 143-159]. Moreover, skin microvascular dysfunction in type 1 diabetes precedes symptoms of end-organ microvascular disease [Khan, F. et al., 2000. Impaired skin microvascular function in children, adolescents, and young adults with type 1 diabetes. Diabetes Care 23, 215-220]. In this study, we assessed skin microvascular function of patients with type 1 diabetes using laser-Doppler perfusion monitoring (LDPM) coupled with physiological and pharmacological local vasodilator stimuli.
Diabetes Unit, State University of Rio de Janeiro, Brazil.
Marilia B
MB
Gomes
Alessandra S M
AS
Matheus
Eduardo
E
Tibiriçá
D000109:Acetylcholine / Q000494:pharmacology; D000328:Adult; D003922:Diabetes Mellitus, Type 1 / Q000503:physiopathology*; D004730:Endothelium, Vascular / Q000187:drug effects / Q000503:physiopathology*; D005260:Female; D006801:Humans; D006940:Hyperemia / Q000503:physiopathology; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008297:Male; D008833:Microcirculation / Q000187:drug effects / Q000503:physiopathology; D008991:Monitoring, Physiologic / Q000379:methods; D009599:Nitroprusside / Q000494:pharmacology; D012867:Skin / Q000098:blood supply* / Q000187:drug effects / Q000503:physiopathology; D014664:Vasodilation / Q000187:drug effects / Q000502:physiology
D009599:Nitroprusside; D000109:Acetylcholine