Method for electroporation for the early chick embryo.
In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures, and some experimental examples are presented.
Hatakeyama
Jun
J
Division of Morphogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
Shimamura
Kenji
K
D000818:Animals; D002642:Chick Embryo; D015509:Developmental Biology / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D046149:Embryo Culture Techniques / Q000379:methods*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D049452:Green Fluorescent Proteins