FKBP12 deficiency reduces strength deficits after eccentric contraction-induced muscle injury.
Strength deficits associated with eccentric contraction-induced muscle injury stem, in part, from excitation-contraction uncoupling. FKBP12 is a 12-kDa binding protein known to bind to the skeletal muscle sarcoplasmic reticulum Ca2+ release channel [ryanodine receptor (RyR1)] and plays an important role in excitation-contraction coupling. To assess the effects of FKBP12 deficiency on muscle injury and recovery, we measured anterior crural muscle (tibialis anterior and extensor digitorum longus muscles) strength in skeletal muscle-specific FKBP12-deficient and wild-type (WT) mice before and after a single bout of 150 eccentric contractions, as well as before and after the performance of six injury bouts. Histological damage of the tibialis anterior muscle was assessed after injury. Body weight and peak isometric and eccentric torques were lower in FKBP12-deficient mice compared with WT mice. There were no differences between FKBP12-deficient and WT mice in preinjury peak isometric and eccentric torques when normalized to body weight, and no differences in the relative decreases in eccentric torque with a single or multiple injury bouts. After a single injury bout, FKBP12-deficient mice had less initial strength deficits and recovered faster (especially females) than WT mice, despite no differences in the degree of histological damage. After multiple injury bouts, FKBP12-deficient mice recovered muscle strength faster than WT mice and exhibited significantly less histological muscle damage than WT mice. In summary, FKBP12 deficiency results in less initial strength deficits and enhanced recovery from single (especially females) and repeated bouts of injury than WT mice.
Georgia State University, Department of Kinesiology and Health, P. O. Box 3975, Atlanta, GA 30302-3975, USA.
Benjamin T
BT
Corona
Clement
C
Rouviere
Susan L
SL
Hamilton
Christopher P
CP
Ingalls
D000818:Animals; D001835:Body Weight / Q000502:physiology; D002110:Caffeine / Q000494:pharmacology; D000697:Central Nervous System Stimulants / Q000494:pharmacology; D004558:Electric Stimulation; D005260:Female; D007537:Isometric Contraction; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009119:Muscle Contraction / Q000502:physiology; D053580:Muscle Strength / Q000235:genetics* / Q000502:physiology*; D018482:Muscle, Skeletal / Q000033:anatomy & histology / Q000293:injuries*; D009929:Organ Size / Q000502:physiology; D012727:Sex Characteristics; D022061:Tacrolimus Binding Protein 1A / Q000172:deficiency* / Q000235:genetics*
D000697:Central Nervous System Stimulants; D002110:Caffeine; D022061:Tacrolimus Binding Protein 1A