Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B.
Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunoprecipitation and confocal immunofluorescence analysis in cells with transient expression of NS5B and M2PK as well as in a HCV replicon-bearing cell line. Furthermore shRNA which specifically down-regulated M2PK expression could inhibit the replication of HCV in HCV replicon 9B cells.
State Key Laboratory of Virology, the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China.
Xiaoyun
X
Wu
You
Y
Zhou
Ke
K
Zhang
Qingzhen
Q
Liu
Deyin
D
Guo
D000818:Animals; D002460:Cell Line; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism*; D012093:Replicon; D020798:Two-Hybrid System Techniques; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase
D007527:Isoenzymes; D011770:Pyruvate Kinase; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase