[Effect of advanced glycosylation end products on cell cycle of epidermal keratinocyte and the role of signal pathway].
OBJECTIVE
To investigate the effect of advanced glycosylation end products (AGE) on cell cycle of epidermal keratinocyte and its possible signal pathway.


METHODS
150 mg/L AGE-human serum albumin (AGE-HSA) was prepared in vitro. Primary cultured keratinocytes in logarithmic growth phase were harvested and divided randomly into: A group [with treatment of defined keratinocyte-SFM (DK-SFM) serum-free medium], B group (with treatment of DK-SFM medium including 150 mg/L AGE-HSA), C group (with DK-SFM medium after treatment of U0126) and group D (with D K-SFM medium including 150 mg/L AGE-HSA after treatment of U0126). Cell cycle distributions were analyzed by flow cytometer. The protein levels of cyclin D1, cyclin B1, CDK4 and p44/42 MAPK were measured by Western blot.


RESULTS
Compared with those of A group, the percentage of S-phase and G2/M-phase keratinocytes were decreased obviously in B group, the percentages of G2/M -phase keratinocytes showed the same tendency in C and D groups [(9.7 +/- 1.1)% , (9.8 +/- 0.7)%, respectively, P <0.05]. Compared with that of A group, the expression of cyclin D1 were decreased significantly in other groups, among which a weak expression was showed in D group. There was no obvious difference between A and B groups in CDK4, or cyclin B1 and p44/42 MAPK protein levels ,which were significantly higher than those in C and D groups.


CONCLUSIONS
AGEs inhibit the progress of cell cycle of keratinocytes by downregulation of cyclin D1 expression.
Department of Burns, the Third Hospital of Wuhan, Wuhan 430060, PR China.
Ting
T
Xie
Yi-Wen
YW
Niu
Kui
K
Ge
Shu-Liang
SL
Lu
D000818:Animals; D002453:Cell Cycle; D019938:Cyclin D1 / Q000378:metabolism*; D000078404:Epidermal Cells; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism / Q000494:pharmacology*; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction
D017127:Glycation End Products, Advanced; D019938:Cyclin D1; D048049:Extracellular Signal-Regulated MAP Kinases