Effects of Triton X-100 nanoaggregates on dimerization and antioxidant activity of morin.
Dimerization and antioxidant activity of morin in the Triton X-100 micelles were studied by electronic absorption, ATR-FTIR spectra, cyclic voltammetric, DSC, freeze-fracture TEM, molecular modeling and ab initio quantum calculations. Morin can be solubilized in the Triton X-100 micelles and show selective dimerization in Triton X-100 micelles with different structures. In Triton X-100 spherical micelles, morin always exists in the form of dimer, and in Triton X-100 rodlike micelles, it is always in the form of monomer. The solubilization of morin dimer in Triton X-100 spherical micelles changes the micelle morphology from spherical to cubelike, and the size of the single micelle is also increased, while morin monomer links the Triton X-100 rodlike micelles and forms a kind of network micelle structure with the size of the "rod" unchanged. Solubilized and concentrated in Triton X-100 micelles, morin can protect human serum albumin from the damage induced by hydroxyl radicals effectively and even can form a kind of protein complex with human serum albumin showing more thermal stability.
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, People's Republic of China.
Weiya
W
Liu
Rong
R
Guo
D000975:Antioxidants / Q000737:chemistry*; D002152:Calorimetry, Differential Scanning; D019295:Computational Biology; D019281:Dimerization; D005419:Flavonoids / Q000737:chemistry*; D006801:Humans; D017665:Hydroxyl Radical / Q000737:chemistry; D008823:Micelles; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D017830:Octoxynol / Q000737:chemistry*; D012709:Serum Albumin / Q000737:chemistry / Q000648:ultrastructure; D013053:Spectrophotometry; D047309:Flavones
D000975:Antioxidants; D005419:Flavonoids; D017665:Hydroxyl Radical; D008823:Micelles; D017830:Octoxynol; D012709:Serum Albumin; C008548:morin; D047309:Flavones