Effects of Mirasol PRT treatment on storage lesion development in plasma-stored apheresis-derived platelets compared to untreated and irradiated units.
BACKGROUND
The aim of this study was to examine the effects of a new riboflavin-based pathogen reduction technology (PRT), the Mirasol PRT process (Navigant Biotechnologies) on platelet (PLT) storage lesion development.


METHODS
A three-arm in vitro study was conducted comparing cell quality of apheresis PLTs (n = 12 each) treated with Mirasol PRT (M) to untreated (C) and gamma-irradiated units (X) collected from the same donors and stored for up to 7 days under equal conditions.


RESULTS
PLT count, lactate dehydrogenase, and K+ release of M units were not significantly different from C units, indicating retention of cell integrity during storage. The immediate effect (Day 1) of PRT treatment was a significant decrease in hypotonic shock response (M, 80.6 +/- 7.8% vs. 89.2 +/- 8.3%) and aggregation (M, 85.7 +/- 15.2%/min vs. 111.8 +/- 31.5%/min) as well as a significant acceleration of mitochondrial membrane depolarization (M, 1.43 +/- 0.44% vs. 0.91 +/- 0.27%) and P-selectin expression (M, 38.4 +/- 13.8% vs. 15.8 +/- 7.7%) resulting in lower swirl scores on Day 5 (1.5 +/- 0.7 vs. 2.7 +/- 0.4). Significantly higher glucose consumption (60 +/- 13 nmol/10(12) cells/hr vs. 31 +/- 9 nmol/10(12) cells/hr) and lactate production rates (82 +/- 17 nmol/10(12) cells/hr vs. 40 +/- 8 nmol/10(12) cells/hr) caused higher acidity in treated units (pH on Day 5, 6.97 +/- 0.15 vs. 7.42 +/- 0.10). After PRT treatment, oxidative metabolism was still active and, from calculation of oxygen consumption (1.09 +/- 0.23 nmol/min/10(9) PLTs), appeared to be up regulated relative to controls (0.76 +/- 0.27 nmol/min/10(9) PLTs).


CONCLUSIONS
Although storage variables clearly showed the effects of PRT treatment, apheresis PLTs treated with Mirasol PRT retained cell quality during 5 days of storage without loss of mitochondria-based oxidative respiration.
Picker
Susanne M
SM
Transfusion Medicine, University of Cologne, Cologne, Germany. susanne.picker@uk-koeln.de
Steisel
Alexander
A
Gathof
Birgit S
BS
D016470:Bacteremia / Q000517:prevention & control*; D001781:Blood Component Removal; D001792:Blood Platelets / Q000166:cytology* / Q000187:drug effects / Q000528:radiation effects; D001793:Blood Preservation; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D011564:Furocoumarins; D005720:Gamma Rays; D005937:Glucaric Acid / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D007773:Lactates / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects / Q000528:radiation effects; D010976:Platelet Count; D017713:Platelet Transfusion; D012256:Riboflavin
D011564:Furocoumarins; D007773:Lactates; D007770:L-Lactate Dehydrogenase; C118577:amotosalen; D005937:Glucaric Acid; D012256:Riboflavin