Dissecting the roles of aquaporins in renal pathophysiology using transgenic mice.
Transgenic mice lacking renal aquaporins (AQPs), or containing mutated AQPs, have been useful in confirming anticipated AQP functions in renal physiology and in discovering new functions. Mice lacking AQPs 1-4 manifest defects in urinary concentrating ability to different extents. Mechanistic studies have confirmed the involvement of AQP1 in near-isosmolar fluid absorption in the proximal tubule, and in countercurrent multiplication and exchange mechanisms that produce medullary hypertonicity in the antidiuretic kidney. Deletion of AQPs 2-4 impairs urinary concentrating ability by reduction of transcellular water permeability in the collecting duct. Recently created transgenic mouse models of nephrogenic diabetes insipidus produced by AQP2 gene mutation offer exciting possibilities to test new drug therapies. Several unanticipated AQP functions in kidney have been discovered recently that are unrelated to their role in transcellular water transport. There is evidence for involvement of AQP1 in kidney cell migration after renal injury, of AQP7 in renal glycerol clearance, of AQP11 in prevention of renal cystic disease, and possibly of AQP3 in regulation of collecting duct cell proliferation. Future work in renal AQPs will focus on mechanisms responsible for these non-fluid-transporting functions, and on the development of small-molecule AQP inhibitors for use as aquaretic-type diuretics.
Verkman
A S
AS
Department of Medicine, Cardiovascular Research Institute, University of California, San Francisco, CA 94143-0521, USA. Alan.Verkman@ucsf.edu <Alan.Verkman@ucsf.edu>
D000818:Animals; D051399:Aquaporin 2 / Q000235:genetics; D020346:Aquaporins / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement; D004195:Disease Models, Animal; D017353:Gene Deletion; D005990:Glycerol / Q000378:metabolism; D007674:Kidney Diseases / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology* / Q000652:urine; D051379:Mice; D008822:Mice, Transgenic; D009154:Mutation
C491927:Aqp7 protein, mouse; D051399:Aquaporin 2; D020346:Aquaporins; D005990:Glycerol