Ischemic preconditioning produces systemic protective and adoptively transferable effects.
Ischemic preconditioning directly protects organs from subsequent non-specific injuries. To test for systemic protective effects kidneys from F-344 donor rats went through a short warm ischemic time. Both, clamped and contralateral unclamped kidneys were procured after either a short (15 min) or long (24 h) reperfusion period and transplanted into Lewis rats following a prolonged cold ischemia. To test for transferable effects serum from preconditioned rats was infused either into native donors or recipients. Following a short reperfusion interval protective effects were only evident in previously clamped grafts. However, after a long reperfusion interval protective effects were observed in previously clamped and contralateral unclamped kidneys promoting improved survival, structure, function and reduced inflammation. These effects were not related to heme oxygenase-1 induction or neural transmission as heme oxygenase-1 inhibition or denervation prior to preconditioning did not affect organ protection. These results show that renal ischemic preconditioning is associated with time-dependent local and systemically transferable protection.
Department of General, Visceral, and Transplant Surgery, Charité, Campus Virchow-Clinic, Universitätsmedizin Berlin, Berlin, Germany.
Anja
A
Reutzel-Selke
Johann
J
Pratschke
Paulo N
PN
Martins
Christian
C
Denecke
Anke
A
Jurisch
Katja
K
Kotsch
Andreas
A
Pascher
Peter
P
Neuhaus
Stefan G
SG
Tullius
D000818:Animals; D001803:Blood Transfusion; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006085:Graft Survival; D006419:Heme Oxygenase (Decyclizing) / Q000037:antagonists & inhibitors; D019194:Ischemic Preconditioning / Q000379:methods*; D007668:Kidney / Q000098:blood supply* / Q000293:injuries / Q000294:innervation; D016030:Kidney Transplantation / Q000009:adverse effects / Q000379:methods* / Q000473:pathology; D011524:Protoporphyrins / Q000494:pharmacology; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D015427:Reperfusion Injury / Q000473:pathology / Q000503:physiopathology / Q000517:prevention & control*; D009435:Synaptic Transmission; D013997:Time Factors
D004791:Enzyme Inhibitors; D011524:Protoporphyrins; C017803:zinc protoporphyrin; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat