Determination of mercury in water and fish samples by cold vapor atomic absorption spectrometry after solid phase extraction on agar modified with 2-mercaptobenzimidazole.
A novel solid phase extraction method for the determination of mercury has been developed. The Hg(II) ions were retained on a mini-column packed with agar powder modified with 2-mercaptobenzimidazole at a flow rate of 6 mL min(-1). The retained Hg(II) ions were eluted with 3 mol L(-1) solution of HCl and measured by cold vapor atomic absorption spectrometry (CV-AAS). The effect of different variables such as pH, sample flow rate, amounts of 2-mercaptobenzimidazole loaded on agar and SnCl(2) concentration was investigated and optimum conditions were established. The calibration curve was linear in the range of 0.040-2.40 ng mL(-1) with r=0.9994 (n=8). The limit of detection based on three times the standard deviation (3S(b)) (n=10) obtained under optimum conditions was 0.02 ng mL(-1). The relative standard deviation (R.S.D.) for the determination of 0.4 and 2.0 ng mL(-1) of Hg(II) was 2.6 and 1.9% (n=8), respectively. The method was successfully applied to determine Hg(II) in water, wastewater and fish samples.
Department of Chemistry, College of Science, Shahid Chamran University, Ahvaz, Iran. npourreza@yahoo.com
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Pourreza
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Ghanemi
D000327:Adsorption; D000362:Agar / Q000737:chemistry; D000818:Animals; D001562:Benzimidazoles / Q000032:analysis / Q000737:chemistry; D002138:Calibration; D002623:Chemistry Techniques, Analytical / Q000379:methods; D004867:Equipment Design; D005399:Fishes; D006863:Hydrogen-Ion Concentration; D008628:Mercury / Q000032:analysis / Q000633:toxicity*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013054:Spectrophotometry, Atomic / Q000379:methods; D017971:Tin Compounds / Q000737:chemistry; D014867:Water / Q000737:chemistry
D001562:Benzimidazoles; D017971:Tin Compounds; D014867:Water; C023599:stannous chloride; C030698:2-mercaptobenzimidazole; D000362:Agar; D008628:Mercury