Multiplex fast real-time PCR for quantitative detection and identification of cos- and pac-type Streptococcus thermophilus bacteriophages.
The fermentation of milk by Streptococcus thermophilus is a widespread industrial process that is susceptible to bacteriophage attack. In this work, a preventive fast real-time PCR method for the detection, quantification, and identification of types of S. thermophilus phages in 30 min is described.
Instituto de Productos Lácteos de Asturias (CSIC), 33300 Villaviciosa, Asturias, Spain.
Beatriz
B
del Rio
María Cruz
MC
Martín
Noelia
N
Martínez
Alfonso H
AH
Magadán
Miguel A
MA
Alvarez
D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D017108:Streptococcus Phages / Q000145:classification / Q000235:genetics*; D048249:Streptococcus thermophilus / Q000821:virology*
D017931:DNA Primers