A reverse transcriptase-dependent mechanism plays central roles in fundamental biological processes.
This review summarizes emerging evidence that LINE-1 (Long Interspersed Nuclear Elements) -encoded reverse transcriptase (RT) regulates fundamental biological processes. Earlier studies showed that sperm cells can be used as vectors of both exogenous DNA and RNA molecules in sperm-mediated gene transfer assays. During these studies, a sperm endogenous RT activity was identified, which can reverse-transcribe exogenous RNA directly, or DNA molecules through sequential transcription and reverse transcription. Resulting cDNA copies generated in sperm cells can be delivered to embryos at fertilization, further propagated in tissues as low-copy extrachromosomal structures and transmitted to the progeny in a non-mendelian fashion. Being transcriptionally competent, they can induce phenotypic variations in positive tissues. An RT activity is also present in preimplantation embryos, and its inhibition causes developmental arrest in early preimplantation stages, paralleled by an extensive reprogramming of gene expression. In analogy with this, drug-mediated inhibition of RT activity, or RNA interference-mediated silencing of human LINE-1, reduce cell proliferation and induce differentiation in a variety of cancer cell lines. Furthermore, RT inhibition in vivo antagonizes the growth of human tumors in animal models. As a whole, these data implicate a RT-dependent machinery in the genesis of new genetic information in spermatozoa and in normal and pathological developmental processes.
Istituto Superiore di Sanità, Rome, Italy. cspadaf@tin.it
Corrado
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Spadafora
D000818:Animals; D047108:Embryonic Development / Q000502:physiology*; D018014:Gene Transfer Techniques; D006801:Humans; D040582:Inheritance Patterns / Q000235:genetics; D020084:Long Interspersed Nucleotide Elements / Q000502:physiology*; D008297:Male; D012194:RNA-Directed DNA Polymerase / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D014158:Transcription, Genetic / Q000502:physiology*
D012194:RNA-Directed DNA Polymerase