Polystyrene microbeads modified with an elastin-like biopolymer for stimuli-responsive immunodetection.
An efficient and controlled method for immunodetection, using polystyrene (PS) microbeads conjugated with an elastin-like polypeptide (ELP), was investigated. ELP is a temperature-sensitive polymer that exhibits a hydrophilic-hydrophobic phase transition at the lower critical solution temperature. To introduce amine groups, ELP was modified with lysine for conjugation with PS microbeads functionalized with carboxylic groups. Prostate-specific antigen (PSA), a cancer marker, was detected from a biomolecular mixture using ELP-conjugated PS microbeads and ELP-conjugated antiPSA. External stimuli were used to reversibly separate the PSA-antiPSA complex. The stimuli-responsiveness of the ELP provided a designable generic biosurface for diagnostic detection.
Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul, South Korea.
Okgene
O
Kim
Kyunga
K
Na
Jonghwan
J
Lee
Jaeyeon
J
Jung
Duckjong
D
Kim
Sangjin
S
Park
Kyusik
K
Yun
Jinho
J
Hyun
D004549:Elastin / Q000032:analysis* / Q000737:chemistry* / Q000276:immunology; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D010455:Peptides / Q000032:analysis / Q000737:chemistry / Q000276:immunology; D011137:Polystyrenes / Q000737:chemistry*; D017430:Prostate-Specific Antigen
D010455:Peptides; D011137:Polystyrenes; D004549:Elastin; D017430:Prostate-Specific Antigen