Determination and pharmacokinetics of danshensu in rat plasma after oral administration of danshen extract using liquid chromatography/tandem mass spectrometry.
A liquid chromatography/tandem mass spectrometry (LC/MS/MS) method was developed and validated for the determination and pharmacokinetics of danshensu in rat plasma samples using ferulic acid as internal standard (IS). The plasma samples were treated by liquid-liquid extraction, and the analyses were determined using electrospray negative ionization mass spectrometry in selected reaction monitoring (SRM) mode. The signal intensity of the m/z 196.8 --> 134.8 transition of danshensu was found to relate linearly to danshensu concentrations in the plasma from 5-500 ng/mL. The lower limit of quantification (LLOQ) as determined by the LC/MS/MS method amounted to 5 ng/mL. The intra- and inter-day precision was below 10.82%, and the accuracy was between -3.51% and +11.92%. The validated LC/MS/MS method was applied to a pharmacokinetic study in which danshen extract (containing 40 mg/g danshensu) was administered orally to rats at a single dose of 200 mg/kg in 2% water.
School of Chemical Engineering and Technology, Tianjin University, Tianjin, Republic of China.
Wei
W
Li
Zhi-Wen
ZW
Li
Jian-Ping
JP
Han
Xin-Xin
XX
Li
Jun
J
Gao
Chang-Xiao
CX
Liu
D000284:Administration, Oral; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003373:Coumaric Acids / Q000097:blood / Q000493:pharmacokinetics; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics; D007773:Lactates / Q000097:blood* / Q000493:pharmacokinetics; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
D003373:Coumaric Acids; D004365:Drugs, Chinese Herbal; D007773:Lactates; C035055:3,4-dihydroxyphenyllactic acid; C004999:ferulic acid