Polymer-induced phase separation and crystallization in immunoglobulin G solutions.
We study the effects of the size of polymer additives and ionic strength on the phase behavior of a nonglobular protein-immunoglobulin G (IgG)-by using a simple four-site model to mimic the shape of IgG. The interaction potential between the protein molecules consists of a Derjaguin-Landau-Verwey-Overbeek-type colloidal potential and an Asakura-Oosawa depletion potential arising from the addition of polymer. Liquid-liquid equilibria and fluid-solid equilibria are calculated by using the Gibbs ensemble Monte Carlo technique and the Gibbs-Duhem integration (GDI) method, respectively. Absolute Helmholtz energy is also calculated to get an initial coexisting point as required by GDI. The results reveal a nonmonotonic dependence of the critical polymer concentration rho(PEG) (*) (i.e., the minimum polymer concentration needed to induce liquid-liquid phase separation) on the polymer-to-protein size ratio q (equivalently, the range of the polymer-induced depletion interaction potential). We have developed a simple equation for estimating the minimum amount of polymer needed to induce the liquid-liquid phase separation and show that rho(PEG) (*) approximately [q(1+q)(3)]. The results also show that the liquid-liquid phase separation is metastable for low-molecular weight polymers (q=0.2) but stable at large molecular weights (q=1.0), thereby indicating that small sizes of polymer are required for protein crystallization. The simulation results provide practical guidelines for the selection of polymer size and ionic strength for protein phase separation and crystallization.
Department of Chemical and Biomolecular Engineering, National University of Singapore, The Singapore-MIT Alliance, National University of Singapore, Singapore 117576.
Jianguo
J
Li
Raj
R
Rajagopalan
Jianwen
J
Jiang
D003460:Crystallization; D018360:Crystallography, X-Ray; D007074:Immunoglobulin G / Q000737:chemistry*; D008958:Models, Molecular; D010316:Particle Size; D011092:Polyethylene Glycols / Q000494:pharmacology; D011108:Polymers / Q000494:pharmacology*; D011487:Protein Conformation; D012996:Solutions
D007074:Immunoglobulin G; D011108:Polymers; D012996:Solutions; D011092:Polyethylene Glycols