High-performance liquid chromatographic assay for cinnarizine in human plasma.
The high performance liquid chromatography for the determination of cinnarizine in human plasma is described. The procedure involves liquid-liquid extraction followed by reversed phase high-performance chromatographic analysis with fluorometric detection. The method was validated for accuracy, precision, specificity, linearity, sensitivity, recovery, and stability. No endogenous compounds were found to interfere. The absolute extraction recovery of cinnarizine and clocinizine (internal standard) from plasma samples were 97% and 89%, respectively. The linearity was assessed in the range 1-100 ng/mL. The intra-day and inter-day relative standard deviations were less than 10%, and the accuracy of the assay expressed by bias was in the range 0.14-2.37%. The method was proved to be suitable for human pharmacokinetic studies following single oral dose.
Analytical Departament, Institute of Industrial Organic Chemistry, 6 Annopol Str., 03-236 Warsaw, Poland. krukowska@ipo.waw.pl
Hanna
H
Nowacka-Krukowska
Monika
M
Rakowska
Kinga
K
Neubart
Maria
M
Kobylińska
D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002936:Cinnarizine / Q000097:blood* / Q000737:chemistry; D004355:Drug Stability; D006801:Humans
D002936:Cinnarizine