Coat protein-mediated resistance against an Indian isolate of the Cucumber mosaic virus subgroup IB in Nicotiana benthamiana.
Coat protein (CP) -mediated resistance against an Indian isolate of the Cucumber mosaic virus (CMV) subgroup IB was demonstrated in transgenic lines of Nicotiana benthamiana through Agrobacterium tumefaciens-mediated transformation. Out of the fourteen independently transformed lines developed, two lines were tested for resistance against CMV by challenge inoculations. The transgenic lines exhibiting complete resistance remained symptomless throughout life and showed reduced or no virus accumulation in their systemic leaves after virus challenge. These lines also showed virus resistance against two closely related strains of CMV. This is the first report of CP-mediated transgenic resistance against a CMV subgroup IB member isolated from India.
Srivastava
A
A
Molecular Virology Lab, National Botanical Research Institute, Lucknow 226 001, India.
Raj
S K
SK
D016960:Agrobacterium tumefaciens / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics*; D017799:Cucumovirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D004797:Enzyme-Linked Immunosorbent Assay; D015151:Immunoblotting; D010935:Plant Diseases / Q000821:virology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000821:virology*; D014026:Nicotiana / Q000235:genetics / Q000821:virology*; D014170:Transformation, Genetic
D036022:Capsid Proteins