Bilioduodenal anastomosis in rats with extra-hepatic biliary obstruction is followed by lesions ischemia and reperfusion-induced.
OBJECTIVE
The aim of this study was to investigate alterations compatible with hepatic ischemia-reperfusion after bilioduodenal shunt (BD) in rats with obstructive jaundice (OB) .


METHODS
Thirty six animals were divided into 6 experimental groups: CO1 and CO2--control groups, sham-operated (SO) and evaluated 6 and 24 hours after, respectively; OB1 and OB2,--obstructive jaundice groups, sham-operated 15 days after bile duct ligature and evaluated 6 and 24 hours after SO, respectively; DBD1 and DBD2--obstructive jaundice groups evaluated ,respectively, 6 and 24 hours after BD performed 15 days after bile duct ligature. The parameters evaluated were serum total bilirubin, aminotransferase activity (AST, ALT), TNFalpha, liver mitochondrial functions and parenchymatous injury.


RESULTS
Bilirubin decreased while aminotransferase activity increased 6 hours after BD (p<0.01); TNFalpha determination at the 6th hour after BD was higher than the one at the 24th hour (p<0.05); oxygen consumption in states 3 and 4 remained elevated in the BD initial phase , and liver cell damage worsened 24 hours after BD.


CONCLUSIONS
The results demonstrated that surgical biliary decompression in obstructive jaundice is followed by alterations related to hepatic ischemia- reperfusion.
Department of Surgery and Anatomy, Ribeirão Preto Faculty of Medicine, University of São Paulo, SP, Brazil.
Márcio Augusto
MA
Ferreira
José Sebastião dos
JS
Santos
Robson Azevedo
RA
Dutra
Wilson
W
Salgado
Rafael
R
Kemp
Camila
C
Domiciano
Leandra N Z
LN
Ramalho
Ajith Kumar
AK
Sankarankutty
Orlando de
Od
Castro-e-Silva
D000714:Anastomosis, Surgical / Q000009:adverse effects; D000818:Animals; D001652:Bile Ducts / Q000601:surgery; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000097:blood; D001651:Cholestasis, Extrahepatic / Q000601:surgery*; D004195:Disease Models, Animal; D004386:Duodenum / Q000601:surgery; D041781:Jaundice, Obstructive / Q000209:etiology / Q000601:surgery*; D008099:Liver / Q000098:blood supply* / Q000378:metabolism / Q000473:pathology; D008297:Male; D010101:Oxygen Consumption / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000150:complications*; D013997:Time Factors; D000637:Transaminases / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D015415:Biomarkers; D014409:Tumor Necrosis Factor-alpha; D000637:Transaminases; D001663:Bilirubin