Capture of flowing endothelial cells using surface-immobilized anti-kinase insert domain receptor antibody.
In humans, self-endothelialization of synthetic grafts is severely limited, but a recent interesting idea is to attract endothelial progenitor cells (EPCs) from peripheral blood onto grafts via antibodies directed at proposed EPC markers. Results with anti-CD34 antibodies have shown some promise, but it is unclear whether CD34 is the best marker for cells with re-endothelializing potential. Much evidence points to kinase insert domain receptor (KDR) as an important indicator of endothelial potential if not a definitive marker. Because KDR is not an adhesion molecule (like CD34), we first demonstrated the ability to use adsorbed and protein G-oriented antibody to this receptor to capture flowing cells onto a solid surface. Using endothelial cells and smooth muscle cells, we show in a model system under low shear rates the ability to selectively capture cells by this receptor. Furthermore, our results indicate that concomitant flow of cells lacking the receptor does not affect the efficiency of capture of KDR(+) cells but that orienting the antibody significantly increases the efficiency of capture.
Markway
Brandon D
BD
Department of Biomedical Engineering, Oregon Health and Science University, Portland, Oregon 97239, USA.
McCarty
Owen J T
OJ
Marzec
Ulla M
UM
Courtman
David W
DW
Hanson
Stephen R
SR
Hinds
Monica T
MT
D000818:Animals; D018952:Antigens, CD34 / Q000096:biosynthesis / Q000737:chemistry*; D001792:Blood Platelets / Q000378:metabolism; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000378:metabolism; D006801:Humans; D008954:Models, Biological; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D010215:Papio; D017434:Protein Structure, Tertiary; D013499:Surface Properties; D013927:Thrombosis / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods*; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D018952:Antigens, CD34; D053773:Transforming Growth Factor beta1