Modulation of P2X7 receptor expression in macrophages from mineral oil-injected mice.
P2X7 receptor activation is involved in a number of pro-inflammatory responses in macrophages and other immune cells. Their expression can be positively modulated with lipopolysaccharide (LPS) and TNFalpha, reinforcing their role during inflammation. We investigated the effect of substances capable of recruiting macrophages into the peritoneal cavity of mice (mineral oil and thioglycolate) on P2X7 receptor expression and function, addressing whether these stimuli can interfere with multinucleated giant cell (MGC) formation, ATP-induced apoptosis, plasma membrane permeabilization and nitric oxide production. It was demonstrated that mineral oil treatment reduces P2X7-dependent MGC formation, whereas thioglycolate treatment does not. Mineral oil treatment reduced P2X7 receptor expression, down-modulating ATP-induced apoptosis, permeabilization and nitric oxide production. In conclusion, mineral oil down modulated P2X7 expression and consequently P2X7-associated phenomena, but thioglycolate did not. These effects might be associated with the unpleasant side effects already described during long-term administration of mineral oil for cosmetic purposes or as a laxative and could be useful in understanding the mechanism of recycling and modulation of P2 receptors present in other situations of immunopathological interest.
Laboratory Imunobiofisica, Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Edifício do Centro de Ciências da Saúde, Cidade Universitária, Ilha do Fundão, Rio de Janeiro, RJ 21941-902, Brazil.
Camila
C
Marques da Silva
Luciana
L
Miranda Rodrigues
Andressa
A
Passos da Silva Gomes
Marcio
M
Mantuano Barradas
Flávia
F
Sarmento Vieira
Pedro Muanis
PM
Persechini
Robson
R
Coutinho-Silva
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002463:Cell Membrane Permeability; D015726:Giant Cells / Q000276:immunology* / Q000378:metabolism; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008899:Mineral Oil / Q000494:pharmacology*; D009569:Nitric Oxide / Q000378:metabolism; D018048:Receptors, Purinergic P2 / Q000172:deficiency / Q000378:metabolism*; D058486:Receptors, Purinergic P2X7; D013864:Thioglycolates / Q000494:pharmacology*
C546469:P2rx7 protein, mouse; D018048:Receptors, Purinergic P2; D058486:Receptors, Purinergic P2X7; D013864:Thioglycolates; D009569:Nitric Oxide; D008899:Mineral Oil; D000255:Adenosine Triphosphate