Ogataea ganodermae sp. nov., a methanol-assimilating yeast species isolated from basidiocarps of Ganoderma sp.
Three methanol-utilizing yeast strains were isolated from basidiocarps of Ganoderma sp. collected from a tree trunk in Mangshan Mountain, Hunan Province, southern China. These strains formed hat-shaped ascospores in unconjugated and deliquescent asci. Sequence analysis of the large-subunit rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region, electrophoretic karyotype comparison and phenotypic characterization demonstrated that the three strains represent a novel species of the genus Ogataea, which is described as Ogataea ganodermae sp. nov. (type strain SHS 2.1(T) =CGMCC AS 2.3435(T) =CBS 10646(T)). Phylogenetically, the novel species was closely related to Ogataea pini and Ogataea henricii. The latter two taxa with similar D1/D2 sequences were confirmed to represent separate species by ITS sequence and electrophoretic karyotype comparisons.
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Zhao-Hui
ZH
Ji
Feng-Yan
FY
Bai
D002681:China; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D048690:Fruiting Bodies, Fungal; D032902:Ganoderma; D020459:Genes, rRNA; D007621:Karyotyping; D000432:Methanol / Q000378:metabolism*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010641:Phenotype; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013172:Spores, Fungal / Q000502:physiology
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D000432:Methanol