Substance P stimulates production of interleukin 1beta and tumor necrosis factor alpha in fibroblasts from hip periprosthetic membrane.
Aseptic loosening remains the primary cause of failure in total joint arthroplasty. Substance P (SP)-immunoreactive nerve fibers have been detected in the pseudomembrane and pseudocapsular tissues of aseptic loose hip prostheses, suggesting that SP might be involved in the process of aseptic loosening. We isolated fibroblasts from periprosthetic membrane at the time of revision hip arthroplasty performed because of aseptic loosening. Fibroblasts were incubated in the presence of various concentrations of SP, and the levels of interleukin 1beta, and tumor necrosis factor alpha in the media were determined using enzyme-linked immunosorbent assay kit. We found that the levels of interleukin 1beta and tumor necrosis factor alpha increased in a time- and concentration-dependent manner. Our results suggested that SP might be involved in the pathogenesis of aseptic loosening.
Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital, Medical College of Shanghai Jiao Tong University, Shanghai, China.
Yebin
Y
Qian
Bing-Fang
BF
Zeng
Xian-Long
XL
Zhang
Yao
Y
Jiang
D000368:Aged; D000369:Aged, 80 and over; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000276:immunology; D006622:Hip Prosthesis; D006801:Humans; D053583:Interleukin-1beta / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D010014:Osteolysis / Q000276:immunology; D011475:Prosthesis Failure; D013268:Stimulation, Chemical; D013373:Substance P / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism*
D053583:Interleukin-1beta; D014409:Tumor Necrosis Factor-alpha; D013373:Substance P