Expression in yeast of secreted lignin peroxidase with improved 2,4-dichlorophenol degradability by DNA shuffling.
Lignin peroxidase (LiP) from Phanerochaete chrysosporium was shown to mineralize a variety of recalcitrant aromatic compounds and oxidize a number of polycyclic aromatic and phenolic compounds. The major problem of the wild type LiP is that it can be inactivated by excess H(2)O(2) and high concentrations of aromatic compounds. We applied a directed evolution technique coupled with a rapid colorimetric screening method to obtain mutant genes with improved H(2)O(2) stability and polychlorinated phenol degradability, and they were successfully expressed as the secretive LiPs in recombinant Saccharomyces cerevisiae. The resulting variants showed approximately 1.6-fold improved 2,4-dichlorophenol (2,4-DCP) degradation activity and stability against H(2)O(2) compared with the parent strain. The kinetic properties of the variants toward 2,4-DCP and H(2)O(2) were also increased compared with the wild type for all three mutants studied. Amino acid sequence analysis indicated that the greatest number of amino acid substitutions was located near the surface or Ca(2+) binding sites of the enzyme.
Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Seocho-gu, Seoul, Korea.
Kang
K
Ryu
Jung Hye
JH
Kang
Lishi
L
Wang
E K
EK
Lee
D019943:Amino Acid Substitution; D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000378:metabolism*; D003124:Colorimetry; D003470:Culture Media; D032621:DNA Shuffling; D007700:Kinetics; D009154:Mutation / Q000235:genetics; D010544:Peroxidases / Q000378:metabolism*; D020075:Phanerochaete / Q000201:enzymology*; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000235:genetics*
D002733:Chlorophenols; D003470:Culture Media; D010544:Peroxidases; C042858:lignin peroxidase; C004762:2,4-dichlorophenol