[The preliminary analysis of the recognition epitopes of anti-HEV monoclonal antibodies on HEV ORF2].
Western blot, capture-PCR, blocking ELISA and synthetic polypeptides were used to systematically study the recognition epitopes on HEV ORF2 of 23 anti-HEV monoclonal antibodies(McAbs) which were previously generated in our laboratory directed against HEV ORF2. Results showed that seven McAbs recognized linear epitopes that located at aa408-458 of HEV ORF2 and 16 conformation-dependent McAbs, most of which recognized the surface epitopes of native HEV, located at aa459-606 of HEV ORF2. The systematical study of the recognition epitopes of anti-HEV McAbs on HEV ORF2 provides important information for the investigation of virus receptor and HEV infection mechanism, as well as its vaccine and diagnostics development.
Xiong
Jun-Hui
JH
National Institute of Diagnostics and Vaccine Development of Infectious Disease, College of Life Science of Xiamen University, Xiamen 361005, China.
Guo
Qing-Shun
QS
Ge
Sheng-Xiang
SX
Gu
Ying
Y
Chen
Yi-Xin
YX
Miao
Ji
J
Du
Hai-Lian
HL
Shi
Wei-Guo
WG
Zhang
Jun
J
Xia
Ning-Shao
NS
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006508:Hepatitis Antibodies / Q000276:immunology*; D016752:Hepatitis E virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006508:Hepatitis Antibodies; C400971:ORF2 protein, Hepatitis E virus; D014764:Viral Proteins