A new colorimetric test for solid-phase amines and thiols.
A new, efficient, sensitive, and reliable color test for the visual detection of resin-bound primary and secondary amines is described. The reaction between amines and 1-methyl-2-(4'-nitrophenyl)-imidazo[1,2- a]pyrimidinium perchlorate (DESC) provides the "on-bead generated" colored stable intermediate azadiene. The developed protocols allow detection of resin-bound primary amines in the presence of secondary amines. The test can also be used for the detection of resin bound thiols.
Claerhout
Stijn
S
Laboratory for Organic and Microwave-Assisted Chemistry (LOMAC), Department of Chemistry, University of Leuven, Celestijnenlaan 200F, B-3001 Leuven, Belgium.
Ermolat'ev
Denis S
DS
Van der Eycken
Erik V
EV
D000588:Amines / Q000032:analysis* / Q000737:chemistry*; D001372:Aza Compounds / Q000737:chemistry; D017606:Chlorine Compounds / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D015394:Molecular Structure; D013438:Sulfhydryl Compounds / Q000032:analysis* / Q000737:chemistry*
D000588:Amines; D001372:Aza Compounds; D017606:Chlorine Compounds; D013438:Sulfhydryl Compounds