HLA-DRB1, DQA1 and DQB1 Alleles and Haplotypes Frequencies in Iranian Healthy Adult Responders and Non-responders to Recombinant Hepatitis B Vaccine
Different studies have demonstrated that a small proportion of healthy individuals receiving the hepatitis B (HB) vaccine do not produce protective levels of anti-HB antibody, a phenomenon which could be linked to certain human leukocyte antigen (HLA) class-II alleles or haplotypes.



The present study was undertaken to determine the frequency of HLA class-II alleles in Iranian healthy adult responders and non-responders to HB vaccine.



Twelve non-responders (anti-HBs antibody<10 IU/L) and 46 responders (anti-HBs antibody>100 IU/L) were tissue typed for HLA class-II. HLA-DRB1, DQB1 and DQA1 alleles were determined using polymerase chain reaction based on sequence specific primers (PCR-SSP) technique. Accessibility to excess amount of genomic DNA was possible using Epstein-Barr virus (EBV)-transformed B-cells established from all vaccinees.



Our results demonstrated increased frequencies of HLA- DRB107, DRB103, DRB104, DQB10201, DQA10201 alleles and HLA- DRB107/DQB10201/DQA10201 and DRB104/DQB10302/DQA103011 haplotypes in the non-responder group. Comparison between responders and non-responders revealed only a significant difference for DQB10201 allele (p<0.05).



These findings confirm the association of certain HLA alleles and haplotypes with the lack of antibody response to HB vaccine in an Iranian population.
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
Ali Akbar
AA
Amirzargar
Nilufar
N
Mohseni
Mohammad Ali
MA
Shokrgozar
Zohreh
Z
Arjang
Nahid
N
Ahmadi
Manijeh
M
Yousefi Behzadi
Amir
A
Amanzadeh
Fazel
F
Shokri
D000328:Adult; D000483:Alleles; D005260:Female; D006683:HLA-DQ Antigens / Q000235:genetics / Q000276:immunology*; D006684:HLA-DR Antigens / Q000235:genetics / Q000276:immunology*; D006239:Haplotypes / Q000235:genetics / Q000276:immunology*; D006262:Health; D017325:Hepatitis B Vaccines / Q000235:genetics / Q000276:immunology*; D006801:Humans; D008297:Male; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology*
D006683:HLA-DQ Antigens; D006684:HLA-DR Antigens; D017325:Hepatitis B Vaccines; D014614:Vaccines, Synthetic