Characterisation of protein composition and detection of IgA in cervicovaginal fluid by microchip technology.
In this paper the application of microchip electrophoresis to examine the protein profile of cervicovaginal fluid and the detection of IgA heavy and light chains is presented. This method is a fast growing field of technology and ensures high-speed analysis requiring only microliters of sample. Proteins with wide range of molecular masses could be separated within 1 min. Cervicovaginal specimens of healthy women showed a complex protein pattern-containing several peaks in the 15-70 kDa region. sIgA is considered to be an important protein constituent of all mucosal surfaces. Detection of sIgA in cervicovaginal samples was achievable by microchip technology. Under reduced circumstances (induced by mercaptoethanol, a component of the denaturating solution) the disulfide bonds connecting IgA heavy and light chains are broken up and chains can be detected as separate peaks during electrophoresis. In 82.5% of the cases only the light chain of IgA could be detected in the clinical samples. The intact IgA heavy chain could be demonstrated in only 12.5% of the cases. Based on our data some conclusions were provided about the correlation of these patterns with the age of patients, pH of the cervicovaginal fluid, operations performed before sample collection and usage of oral contraceptives.
Department of Obstetrics and Gynaecology, Faculty of Medicine, University of Pécs, Pécs, Hungary.
József
J
Werling
Béla
B
Kocsis
Diane
D
Dean
Ildikó
I
Kustos
D000328:Adult; D002584:Cervix Uteri / Q000276:immunology* / Q000378:metabolism; D046209:Electrophoresis, Microchip; D045604:Extracellular Fluid / Q000276:immunology; D005260:Female; D006801:Humans; D007071:Immunoglobulin A, Secretory / Q000032:analysis* / Q000737:chemistry* / Q000302:isolation & purification; D007147:Immunoglobulin Light Chains / Q000032:analysis; D007137:Immunoglobulin alpha-Chains / Q000032:analysis; D008875:Middle Aged; D014621:Vagina / Q000276:immunology* / Q000378:metabolism
D007071:Immunoglobulin A, Secretory; D007147:Immunoglobulin Light Chains; D007137:Immunoglobulin alpha-Chains