Gibberella ear rot of maize (Zea mays) in Nepal: distribution of the mycotoxins nivalenol and deoxynivalenol in naturally and experimentally infected maize.
The fungus Fusarium graminearum (sexual stage Gibberella zeae) causes ear rot of maize (Zea mays) and contamination with the 8-ketotrichothecenes nivalenol (1) or 4-deoxynivalenol (2), depending on diversity of the fungal population for the 4-oxygenase gene (TRI13). To determine the importance of 1 and 2 in maize ear rot, a survey of naturally contaminated maize in Nepal was combined with experiments in the field and in a plant growth room. In the survey, 1 contamination was 4-fold more frequent than 2 contamination and 1-producers (TRI13) were isolated more than twice as frequently as 2-producers (Psi TRI13). In maize ear rot experiments, genetically diverse 1-producers and 2-producers caused ear rot and trichothecene contamination. Among strains with the same genetic background, however, 1-producers caused less ear rot and trichothecene contamination than did 2-producers. The high frequency of 1 contamination and the high virulence of many 1-producers are of concern because maize is a staple food of rural populations in Nepal and because 1 has proven to be more toxic than 2 to animals.
Desjardins
Anne E
AE
Mycotoxin Research, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Peoria, Illinois 61604, USA. anne.desjardins@ars.usda.gov
Busman
Mark
M
Manandhar
Gyanu
G
Jarosz
Andrew M
AM
Manandhar
Hira K
HK
Proctor
Robert H
RH
D005638:Fruit / Q000382:microbiology; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D009183:Mycotoxins / Q000737:chemistry / Q000378:metabolism*; D009390:Nepal; D010105:Oxygenases / Q000235:genetics / Q000378:metabolism; D010802:Phylogeny; D010935:Plant Diseases / Q000382:microbiology*; D014255:Trichothecenes / Q000737:chemistry / Q000378:metabolism*; D014481:United States; D003313:Zea mays / Q000382:microbiology*
D005656:Fungal Proteins; D009183:Mycotoxins; D014255:Trichothecenes; C038405:nivalenol; D010105:Oxygenases; C007262:deoxynivalenol