[Experimental study on gelatin/polycaprolactam composite nanofiber scaffold in wound healing].
OBJECTIVE
To explore the effects of the gelatin (Gt)/(polycaprolactam) PCL composite nanofiber scaffold on wound healing of full-thickness defect in rabbits.


METHODS
Sixteen rabbits were inflicted with full-thickness skin open wounds on the back, and they were divided into A group (with treatment of Gt/PCL nanofiber membrane, 8 wounds), B group (with treatment of PCL fiber membrane, 8 wounds), and C group (with treatment of vaseline gauze, 8 wounds), the wounds in A , B groups served as self controls. Wound healing time was recorded. Wound healing rates were calculated on 3, 7, 10 post operation day (POD). Wound specimens and their adjacent skin were examined histologically on 3, 7, and 10 POD.


RESULTS
There was obvious difference in wound healing time among A, B, C groups, and they were (18.2 +/- 1.3) d, (20.3 +/- 1.1) d, (22.0 +/- 0.6) d, respectively. Wound healing rate in A group was obviously higher than that in B, C group on 3, 7, 10 POD (P < 0.05). Compared with those in other groups, the proliferation of granulation tissue in dermis in A group was less, the epidermal cells were proliferated quickly, and collagen was arranged regularly.


CONCLUSIONS
Gt/PCL composite nanofiber membrane can promote wound healing of full-thickness skin defect in rabbits, which is an ideal material for tissue engineering.
Department of Burns and Plastic Surgery, Xiangya Hospital, Central South University, Changsha 410008, PR China.
Jian-Hong
JH
Long
Wu-Yuan
WY
Tan
Ren-Wu
RW
Jiang
Yan-Zhong
YZ
Zhang
D000818:Animals; D002209:Caprolactam / Q000737:chemistry*; D004195:Disease Models, Animal; D005780:Gelatin / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D011817:Rabbits; D054457:Tissue Scaffolds; D014945:Wound Healing
D002209:Caprolactam; D005780:Gelatin; C000362:capron