Choosing an adipose tissue depot for sampling: factors in selection and depot specificity.
The importance and the role of adipose tissues are now largely expanded not only because the very high occurrence of obesity but also because the emerging view that adipose tissue could be a reservoir of therapeutic cells. A critical examination of the adipose tissue features according to their location shows that sampling is not as easy as previously thought and needs special attention to heterogeneity and differences. We discussed here these different points and give precise protocols to sample the different adipose tissues and manipulate them.
Institut Louis Bugnard, Toulouse, France.
Louis
L
Casteilla
Luc
L
Pénicaud
Béatrice
B
Cousin
Denis
D
Calise
D017667:Adipocytes / Q000166:cytology; D000273:Adipose Tissue / Q000033:anatomy & histology* / Q000378:metabolism / Q000601:surgery*; D000818:Animals; D050218:Body Fat Distribution; D019411:Clinical Laboratory Techniques; D006801:Humans; D051379:Mice; D009765:Obesity; D051381:Rats