MCP-1 -2518 A/G single nucleotide polymorphism in Slovak patients with systemic sclerosis.
Recent study in a group of German patients with SSc has implicated the SNP in the MCP-1 gene (-2518 A to G) as a factor of susceptibility to SSc. Reflecting the need for replication of genetic association studies, we investigated if this SNP is associated with SSc in another Caucasian population. MCP-1 -2518 A/G genotypes were determined using PCR-SSP in 46 SSc patients and in 449 healthy subjects, all unrelated and of Slovak (Slavonic) origin. The distribution of MCP-1 -2518 A/G genotypes complied with the Hardy-Weinberg equilibrium both in patient and healthy control groups. There was no difference in MCP-1 -2518*G allele frequency between SSc patients and healthy subjects (patients: 0.23; controls: 0.24; P > .05). Furthermore, MCP-1 -2518 GG homozygotes were similarly represented among SSc patients and healthy subjects (P > .05). The association of MCP-1 -2518 A/G SNP with SSc observed originally in German population was not replicated in the Slovak population.
Navratilova
Zdenka
Z
Laboratory of Immunogenomics, Department of Immunology, Faculty of Medicine, Palacky University, I. P. Pavlova 6, 775 20 Olomouc, Czech Republic.
Lukac
Jozef
J
Mrazek
Frantisek
F
Kriegova
Eva
E
Bucova
Maria
M
Bosak
Vladimir
V
Petrek
Martin
M
D000293:Adolescent; D000328:Adult; D016022:Case-Control Studies; D018932:Chemokine CCL2 / Q000235:genetics*; D002648:Child; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012595:Scleroderma, Systemic / Q000235:genetics*
C111835:CCL2 protein, human; D018932:Chemokine CCL2