Microbial production of 1,3-propanediol by Klebsiella pneumoniae XJPD-Li under different aeration strategies.
The microbial production of 1,3-propanediol (1,3-PD) by Klebsiella pneumoniae XJPD-Li under different aeration strategies were investigated. In batch fermentation, the results showed that the final concentration of 1,3-PD and yield on glycerol were 13.44 g/l and 0.73 mol/mol under the anaerobic condition (N2, 0.4 vvm), 11.55 g/l and 0.62 mol/mol without aeration, and 8.73 g/l and 0.47 mol/mol under the aerobic condition (air, 0.4 vvm), respectively. Under the aerobic condition, the yield of 1,3-PD on glycerol was the lowest, while the biomass (optical density at 650 nm) was the highest among these three conditions. In the fed-batch culture, the final concentration and the yield of 1,3-PD was 60.82 g/l and 0.61 mol/mol under the anaerobic condition (N2, 0.4 vvm), 56.43 g/l and 0.53 mol/mol without aeration, and 65.26 g/l and 0.56 mol/mol under the aerobic condition. All these three conditions had good productivities of 1,3-PD, which were 3.35 g/lxh under the anaerobic condition (N2, 0.4 vvm), 3.13 g/lxh without aeration, and 3.16 g/lxh under the aerobic condition within the initial 12 h.
Ma
Bin Bin
BB
Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtan, Shihezi University, Shihezi, Xinjiang 832003, People's Republic of China.
Xu
Xiao Lin
XL
Zhang
Gen Lin
GL
Wang
Li Wei
LW
Wu
Min
M
Li
Chun
C
D000388:Air; D003470:Culture Media; D005285:Fermentation; D006836:Hydro-Lyases / Q000378:metabolism; D007711:Klebsiella pneumoniae / Q000378:metabolism*; D011409:Propylene Glycols / Q000378:metabolism*; D013997:Time Factors
D003470:Culture Media; D011409:Propylene Glycols; C041787:1,3-propanediol; D006836:Hydro-Lyases; C020671:glycerol dehydratase