Immunogenic display of diverse peptides on virus-like particles of RNA phage MS2.
The high level of immunogenicity of peptides displayed in dense repetitive arrays on virus-like particles makes recombinant VLPs promising vaccine carriers. Here, we describe a platform for vaccine development based on the VLPs of RNA bacteriophage MS2. It serves for the engineered display of specific peptide sequences, but will also allow the construction of random peptide libraries from which specific binding activities can be recovered by affinity selection. Peptides representing the V3 loop of HIV gp120 and the ECL2 loop of the HIV coreceptor, CCR5, were inserted into a surface loop of MS2 coat protein. Both insertions disrupted coat VLP assembly, apparently by interfering with protein folding, but these defects were suppressed efficiently by genetically fusing coat protein's two identical polypeptides into a single-chain dimer. The resulting VLPs displayed the V3 and ECL2 peptides on their surfaces where they showed the potent immunogenicity that is the hallmark of VLP-displayed antigens. Experiments with random-sequence peptide libraries show the single-chain dimer to be highly tolerant of six, eight and ten amino acid insertions. MS2 VLPs support the display of a wide diversity of peptides in a highly immunogenic format, and they encapsidate the mRNAs that direct their synthesis, thus establishing the genotype/phenotype linkage necessary for recovery of affinity-selected sequences. The single-chain MS2 VLP therefore unites in a single structural platform the selective power of phage display with the high immunogenicity of VLPs.
Peabody
David S
DS
Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA. dpeabody@salud.unm.edu
Manifold-Wheeler
Brett
B
Medford
Alexander
A
Jordan
Sheldon K
SK
do Carmo Caldeira
Jerri
J
Chackerian
Bryce
B
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D015153:Blotting, Western; D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D019281:Dimerization; D004587:Electrophoresis, Agar Gel; D057927:Hydrophobic and Hydrophilic Interactions; D017909:Levivirus / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D051379:Mice; D008958:Models, Molecular; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D019151:Peptide Library; D010455:Peptides / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*; D014176:Protein Biosynthesis; D017433:Protein Structure, Secondary; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000737:chemistry; D014772:Viroids / Q000737:chemistry / Q000235:genetics* / Q000276:immunology*
D036022:Capsid Proteins; D019151:Peptide Library; D010455:Peptides; D012333:RNA, Messenger; D011994:Recombinant Proteins