[Expression of mGluR1 at primary visual cortex of monocular deprivation amblyopia rat and the observing of ultrastructure].
OBJECTIVE
To explore the regulation of expression of mGluR1 and the changes of neuron ultrastructure at primary visual cortex of monocular deprivation amblyopia rat within cortical period.


METHODS
Taking randomized concurrent controlled trail. Establishing the model of monocular deprivation amblyopia rat. After proving the model successful by PVEP, all of the rats were randomly divided into three groups: normal visual cortex, experimental visual cortex and experimental opposite visual cortex. Immunocytochemical technology, electron microscope, photography microscope, computer image analysis, SPSS 11.5 and ANOV were used to get the results.


RESULTS
Compared with the layer IV of normal visual cortex and experimental opposite visual cortex, the area of immunopositive neurons in layer IV of experimental visual cortex are deficiency, there is significant difference between them (P < 0.01). There are no significant differences between the other four corresponding layers (P > 0.05). Morphological abnormals were found in layer IV of experimental visual cortex by observing of ultrastructure.


CONCLUSIONS
The expression of mGluR1 in layer IV of primary visual cortex of monocular deprivation amblyopia is reduced. There are morphological abnormals happened in layer IV of primary visual cortex of monocular derivation amblyopia. Reduced afference of nerve pulse because of monocular deprivation leads to the expression deficiency of mGluR1 in layer IV of the primary visual cortex, then synaptic plasticity happened, then neurons atrophy occurred may be one of the etiopathogenesis of amblyopia.
Department of Ophthalmology, Sanmenxia Huanghe Hospital, Sanmenxia 472000, China. xyyjs@163.com
Zong-qing
ZQ
Wang
Xiang-ling
XL
Liu
Ya-lin
YL
Mu
Ai-qin
AQ
Liu
Xiao-peng
XP
Li
D000550:Amblyopia / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*; D015349:Vision, Monocular; D014793:Visual Cortex / Q000378:metabolism* / Q000648:ultrastructure*
D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1