Green tea catechins suppress the DNA synthesis marker MCM7 in the TRAMP model of prostate cancer.
Green tea catechins (GTCs) exert chemopreventive effects in many cancer models. Several studies implicate the DNA synthesis marker minichromosome maintenance protein 7 (MCM7) in prostate cancer progression, growth and invasion; representing a novel therapeutic target. In this study, we investigated the effect of GTCs on MCM7 expression in the transgenic adenocarcinoma mouse prostate model (TRAMP). DNA microarray, immunohistochemistry and western blot analysis showed that GTCs significantly suppressed MCM7 in the TRAMP mice treated with GTCs. Our study indicates that the cellular DNA replication factor MCM7 is involved in prostate cancer (CaP) and MCM7 gene expression was reduced by GTCs. Together, these results suggest a possible role of GTCs in CaP chemoprevention in which MCM7 plays a critical role.
Department of Interdisciplinary Oncology, H. Lee Moffitt Cancer Center and Research Institute, University of South Florida, Tampa, Florida, USA.
Susan
S
McCarthy
Andrea
A
Caporali
Steve
S
Enkemann
Maurizio
M
Scaltriti
Steve
S
Eschrich
Pierpaola
P
Davalli
Arnaldo
A
Corti
Alice
A
Lee
Jimmy
J
Sung
Timothy J
TJ
Yeatman
Saverio
S
Bettuzzi
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism* / Q000517:prevention & control; D000818:Animals; D014408:Biomarkers, Tumor / Q000096:biosynthesis* / Q000235:genetics; D002392:Catechin / Q000494:pharmacology*; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004261:DNA Replication / Q000187:drug effects*; D004273:DNA, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D064168:Minichromosome Maintenance Complex Component 7; D009374:Neoplasms, Experimental / Q000378:metabolism / Q000517:prevention & control; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000517:prevention & control; D013662:Tea
D014408:Biomarkers, Tumor; D018797:Cell Cycle Proteins; D004273:DNA, Neoplasm; D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D013662:Tea; D002392:Catechin; C491086:Mcm7 protein, mouse; D064168:Minichromosome Maintenance Complex Component 7