Calculation of the free energy barriers in the oligomerisation of Abeta peptide fragments.
Protein misfolding and aggregation are associated with a range of severe human neurodegenerative conditions. We use all-atom simulations to describe the process of assembly of the Abeta(16-22) and Abeta(25-35) fragments of Abeta, a peptide associated with Alzheimer's disease. Our results indicate that the pathways of aggregation of these two peptides depend predominantly on the relative strength of hydrophobic interactions and hydrogen bonding. In the Abeta(25-35) peptide, which is weakly hydrophobic, the tendency to form hydrogen bonds drives the crossing of a single major free energy barrier for the formation of a cross-beta structure. By contrast, in the more hydrophobic Abeta(16-22) peptide, the process of ordered assembly is preceded by an initial collapse into disordered oligomers. These results provide support for a recently proposed two-step mechanism of amyloid formation. We have also found that the barriers for reordering are lower for large oligomers than for small oligomers, a result that provides an explanation of the recent experimental observation that the efficiency of the seeding reaction depends on the size of the seeds themselves.
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, CB2 1EW, UK.
Mookyung
M
Cheon
Giorgio
G
Favrin
Iksoo
I
Chang
Christopher M
CM
Dobson
Michele
M
Vendruscolo
D016229:Amyloid beta-Peptides / Q000737:chemistry*; D001665:Binding Sites; D009842:Oligopeptides / Q000737:chemistry; D010446:Peptide Fragments / Q000737:chemistry*; D011487:Protein Conformation; D013816:Thermodynamics
D016229:Amyloid beta-Peptides; D009842:Oligopeptides; D010446:Peptide Fragments