In vivo efficacy of calceolarioside A against experimental visceral leishmaniasis.
Bioactivity-guided fractionation has led to the successful isolation of calceolarioside A ( 1) from the methanolic extract of night jasmine leaves. The in vitro antileishmanial activity of compound 1 was determined (IC (50) = 20 microg/mL). Its IN VIVO efficacy was noted at 20 mg/kg body weight when it reduced the hepatic and splenic parasite burden by 79 and 84 %, respectively, in an established model of L. donovani Ag83 infected golden hamster. Furthermore, synergistic potentiations of compound 1 at 20 mg/kg body weight and SAG at 5 mg/kg body weight showed a significant reduction of hepatic and splenic parasite burden. No cytotoxicity was observed against the U937 cell line. This is the first report describing the isolation of compound 1 from N. arbor-tristis L. and the first demonstration of its potent activity against visceral leishmaniasis.
Drug Development/Diagnostics & Biotechnology Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Kolkata, India.
Avijit
A
Poddar
Anindita
A
Banerjee
Srijani
S
Ghanta
Sharmila
S
Chattopadhyay
D000818:Animals; D002109:Caffeic Acids / Q000302:isolation & purification / Q000627:therapeutic use*; D006224:Cricetinae; D016166:Free Radical Scavengers / Q000302:isolation & purification; D005960:Glucosides / Q000302:isolation & purification / Q000627:therapeutic use*; D007893:Leishmania donovani; D007898:Leishmaniasis, Visceral / Q000188:drug therapy*; D008297:Male; D008647:Mesocricetus; D029592:Oleaceae / Q000737:chemistry*; D008517:Phytotherapy; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use
D002109:Caffeic Acids; D016166:Free Radical Scavengers; D005960:Glucosides; D010936:Plant Extracts; C083066:calceolarioside A