[Inhibitory effects of ODC and AdoMetDC bi-antisense virus on the growth and invasion of lung cancer cell A-549].
OBJECTIVE
To study the inhibitory effects of antisense bicistronic recombinant adenovirus vector of ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (Ad-ODC-AdoMetDCas) on polyamine biosynthesis,proliferation and invasion of lung cancer cells.


METHODS
Adenovirus-mediated gene transduction efficiency was assessed with counting GFP-positive cells using trypan blue. Western Blot and HPLC were used to detect ODC and S-AdoMetDC expression and polyamine content in A-549 cells respectively. Viable cell counting and cell cycle analysis were adopted to evaluate cell growth and cell cycle distribution, and A-549 cell invasion in vitro was detected with Matrigel invasion assay.


RESULTS
Approximate 75% of A-549 cells were infected with Ad-ODC-AdoMetDCas when multiplicity of infection reached 50. Our study demonstrated that Ad-ODC-AdoMetDCas vector-mediated gene transfer inhibited tumor cell growth through the blockade of polyamine synthesis pathway. The tumor cells were arrested at cell cycle G1 phase after gene transfer. Gene transferred tumor cells were shown to possess markedly decreased invasiveness.


CONCLUSIONS
Ad-ODC-AdoMetDCas has significant inhibitory effects on lung cancer cell proliferation and invasion and bears therapeutic potential for the treatment of lung cancer.
Sun
Dong-Feng
DF
Department of Thoracic Surgery, Shandong University, Qilu Hospital, Jinan 250012, China.
Tian
Hui
H
Liu
Xian-Xi
XX
Zhang
Bing
B
Zhang
Yan
Y
Sun
Qi-Feng
QF
D012437:Adenosylmethionine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D000256:Adenoviridae / Q000235:genetics; D015153:Blotting, Western; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002851:Chromatography, High Pressure Liquid; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009955:Ornithine Decarboxylase / Q000235:genetics* / Q000378:metabolism; D011073:Polyamines / Q000378:metabolism; D016372:RNA, Antisense / Q000235:genetics*; D014162:Transfection
D011073:Polyamines; D016372:RNA, Antisense; D049452:Green Fluorescent Proteins; D009955:Ornithine Decarboxylase; D012437:Adenosylmethionine Decarboxylase