Peripheral projections of NESP55 containing neurons in the rat sympathetic ganglia.
The peripheral projections of neurons expressing neuroendocrine secretory protein 55 (NESP55), a novel member of the chromogranin family, were studied by retrograde tracing technique. It was found that NESP55 positive neurons in the rat superior cervical ganglion projected to a number of targets including the submandibular gland, the cervical lymph nodes, the forehead skin, the iris, but not to the thyroid. Among these NESP55 positive target-projecting neurons, a subpopulation contained neuropeptide Y (NPY), a vasoconstrictor. Forepaw pad projecting neurons were found exclusively in the stellate ganglion, almost all of which (approximately 90%) were immunoreactive to NESP55. Colocalization of NESP55 and calcitonin gene-related peptide (CGRP), a peptide involved in sudomotor effects, was observed in a subpopulation of these paw pad projecting neurons, as was colocalization of NESP55 and NPY. The data suggest that NESP55 may have a functional role in some populations of sympathetic neurons.
Li
Yongling
Y
Department of Anatomy and Cell Biology, Institute of Biomedicine, Göteborg University, Box 420, SE-405 30 Göteborg, Sweden. Electronic address: yongling.li@anatcell.gu.se.
Dahlström
Annica
A
Department of Anatomy and Cell Biology, Institute of Biomedicine, Göteborg University, Box 420, SE-405 30 Göteborg, Sweden.
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002864:Chromogranins; D019205:GTP-Binding Protein alpha Subunits, Gs / Q000378:metabolism*; D005728:Ganglia, Sympathetic / Q000378:metabolism*; D007498:Iris / Q000294:innervation / Q000378:metabolism; D008198:Lymph Nodes / Q000294:innervation / Q000378:metabolism; D008297:Male; D008845:Microinjections; D008856:Microscopy, Fluorescence; D009412:Nerve Fibers / Q000502:physiology; D009434:Neural Pathways / Q000502:physiology*; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D009478:Neuropeptide Y / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000294:innervation / Q000378:metabolism; D013233:Stellate Ganglion / Q000378:metabolism; D013363:Submandibular Gland / Q000294:innervation / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology*; D013961:Thyroid Gland / Q000294:innervation / Q000378:metabolism
D002864:Chromogranins; D009478:Neuropeptide Y; C498643:Gnas protein, rat; D019205:GTP-Binding Protein alpha Subunits, Gs; D015740:Calcitonin Gene-Related Peptide