In vitro whole-organ imaging: 4D quantification of growing mouse limb buds.
Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).
Boot
Marit J
MJ
Medical Research Council Human Genetics Unit, Crewe Road South, Edinburgh EH4 2XU, UK.
Westerberg
C Henrik
CH
Sanz-Ezquerro
Juanjo
J
Cotterell
James
J
Schweitzer
Ronen
R
Torres
Miguel
M
Sharpe
James
J
D000818:Animals; D019521:Body Patterning; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000379:methods*; D066298:In Vitro Techniques; D051379:Mice; D008822:Mice, Transgenic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D041622:Tomography, Optical / Q000379:methods*
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins