Identification and comparative determination of rhodionin in traditional tibetan medicinal plants of fourteen Rhodiola species by high-performance liquid chromatography-photodiode array detection and electrospray ionization-mass spectrometry.
Using the HPLC/PDA/ESI/MS method, a comparative analysis of rhodionin (RH) was undertaken in order to conduct a qualitative and quantitative study in 38 batches of fourteen species of Rhodiola for quality control purposes. Alongside of this RH analysis, a simultaneous determination of salidroside (SA), tyrosol (TY), and gallic acid (GA) was carried out. Rhodiola plants are a popularly used ethnodrug from the Qinghai-Tibetan plateau of China. The identity of RH was unambiguously determined based on the quasimolecular ions in negative ESI-MS mode. This method was validated in respect to sensitivity, linearity, precision, repeatability and recovery using optimized chromatographic conditions. The linear calibration curve was acquired with R(2)>0.999, and the limit of detection (S/N=3) was estimated to be 43.75 microg/g. The relative standard deviations (RSDs) of the intra- and inter-day precisions were 0.75% and 0.50%, respectively. The repeatability was evaluated by a replicated analysis of samples with the RSD value found within 0.67%. The recovery rates varied within the range of 98.79-100.08% with RSD less than 1.10%. In the present study, the content of RH was quantified within 0.4192-4.7260 mg/g for 16 batches of R. crenulata. It was also found in eight other species plants. The results demonstrated that RH is a useful characteristic standard compound for quality evaluation and chemical differentiation among species of Rhodiola. The study also indicated that the analytical procedure is precise, reproducible and a potential tool for both quality assessment and species identification.
West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, PR China.
Tao
T
Li
Hao
H
Zhang
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004563:Electrochemistry; D005419:Flavonoids / Q000737:chemistry*; D007202:Indicators and Reagents; D009005:Monosaccharides / Q000737:chemistry*; D009862:Online Systems; D012015:Reference Standards; D015203:Reproducibility of Results; D029771:Rhodiola / Q000737:chemistry*; D012996:Solutions; D013045:Species Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
D005419:Flavonoids; D007202:Indicators and Reagents; D009005:Monosaccharides; D012996:Solutions; C530045:rhodionin