Beta-adrenergic stimulation enhances the heat-shock response in fish.
We have taken advantage of the unique properties of nucleated rainbow trout (Oncorhynchus mykiss) red blood cells (rbcs) to demonstrate that beta-adrenergic stimulation with the agonist, isoproterenol, significantly enhanced the heat-induced induction of heat-shock proteins (Hsps) in trout rbcs without affecting hsp expression on its own. Furthermore, this beta-adrenergic potentiation of hsp expression occurred only at physiologically relevant concentrations of adrenergic stimulation. In further experiments, we found that adrenaline increased 100-fold and noradrenaline increased 50-fold in trout after a 1-h heat shock at 25 degrees C, approximately 12 degrees C above acclimation temperature. This is the first time the adrenergic heat-shock response has been described for a temperate fish species. We conclude that beta-adrenergic stimulation enhances hsp expression in trout rbcs following heat stress, indicating physiological regulation of the cellular stress response in fish.
Currie
Suzanne
S
Department of Biology, Mount Allison University, Sackville, New Brunswick E4L 1G7, Canada. scurrie@mta.ca
Reddin
Katharine
K
McGinn
Patrick
P
McConnell
Tom
T
Perry
Steve F
SF
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D004837:Epinephrine / Q000097:blood; D004900:Erythroblasts / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D018869:Heat-Shock Response / Q000187:drug effects / Q000502:physiology*; D015151:Immunoblotting; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009638:Norepinephrine / Q000097:blood; D017686:Oncorhynchus mykiss / Q000097:blood / Q000502:physiology*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000318:Adrenergic beta-Agonists; D006360:Heat-Shock Proteins; D012313:RNA; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine