First intron of nestin gene regulates its expression during C2C12 myoblast differentiation.
Nestin is an intermediate filament protein expressed in neural progenitor cells and in developing skeletal muscle. Nestin has been widely used as a neural progenitor cell marker. It is well established that the specific expression of the nestin gene in neural progenitor cells is conferred by the neural-specific enhancer located in the second intron of the nestin gene. However, the transcriptional mechanism of nestin expression in developing muscle is still unclear. In this study, we identified a muscle cell-specific enhancer in the first intron of mouse nestin gene in mouse myoblast C2C12 cells. We localized the core enhancer activity to the 291-661 region of the first intron, and showed that the two E-boxes in the core enhancer region were important for enhancer activity in differentiating C2C12 cells. We also showed that MyoD protein was involved in the regulation of nestin expression in the myogenic differentiation of C2C12 cells.
Shan Dong University Medical School, Jinan 250012, China.
Hua
H
Zhong
Zhigang
Z
Jin
Yongfeng
Y
Chen
Ting
T
Zhang
Wei
W
Bian
Xing
X
Cui
Naihe
N
Jing
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics*; D002460:Cell Line; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D007381:Intermediate Filament Proteins / Q000235:genetics*; D007438:Introns / Q000235:genetics*; D051379:Mice; D032446:Myoblasts / Q000166:cytology* / Q000502:physiology*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D064231:Nestin; D011401:Promoter Regions, Genetic / Q000235:genetics*
D007381:Intermediate Filament Proteins; D009419:Nerve Tissue Proteins; C577485:Nes protein, mouse; D064231:Nestin