[Effects of beta-asarone on expression of c-fos in kindling epilepsy rat brain].
OBJECTIVE
To study the effects of beta-asarone on expression of immediately early gene c-fos in kindling epilepsy rat brain.


METHODS
The rats were randomly divided in to beta-asarone groups (200, 100, 50 mg x kg(-1) x d(-1)), difetoin control group (36 mg x kg(-1)) and model group. The remedy was administered orally. The effects were observed in kindling epilepsy model induced by penicillin, then the expression of c-fos were determined by western blot (hippocampus) and immunohistochemical techniques (cortex).


RESULTS
Beta-asarone could significantly increase the expression of c-fos in kindling epilepsy rat brain, and show its quantity-effect relation. The expression of c-fos in hippocampus was (1139.45 +/- 155.56), (1109.56 +/- 134.03), (1103.73 +/- 235.82) CNT x mm2 in beta-asarone groups, 920.54 +/- 203.20 in model control group, and 1106.26 +/- 186.24 in difetoin group, respectively. The number of c-fos positive cell was 87.1 +/- 2.2, 76.3 +/- 1.3 and 59.9 +/- 1.3 in beta-asarone groups, 39.3 +/- 2.6 in model control group, and 95.2 +/- 1.1 in difetoin group, respectively.


CONCLUSIONS
Beta-asarone can obviously increase the expression of c-fos in epilepsy rat brain. It is one of important response to epilepsy.
The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China. Fangyq2@163.com
Yong-Qi
YQ
Fang
Ruo-Ming
RM
Fang
Geng-Li
GL
Fang
Yong
Y
Jiang
Si-Ying
SY
Fu
D000081248:Allylbenzene Derivatives; D000818:Animals; D000840:Anisoles / Q000494:pharmacology*; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000378:metabolism*; D004827:Epilepsy / Q000188:drug therapy* / Q000378:metabolism*; D005260:Female; D015870:Gene Expression / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
D000081248:Allylbenzene Derivatives; D000840:Anisoles; D016760:Proto-Oncogene Proteins c-fos; C012195:asarone