Cowpea golden mosaic disease in Gujarat is caused by a Mungbean yellow mosaic India virus isolate with a DNA B variant.
It has long been assumed that cowpea golden mosaic disease (CGMD) in southern Asia is caused by a begomovirus distinct from those causing disease in other legumes. The components of a begomovirus causing CGMD in western India were isolated, cloned and sequenced. Analysis of the sequences shows the virus to be an isolate of Mungbean yellow mosaic India virus, but with a distinct DNA B component with greater similarity to components of a second legume-infecting begomovirus occurring in the region, Mungbean yellow mosaic virus. The clones of the virus were readily infectious to cowpea, mungbean, blackgram and French bean by agroinoculation. However, the wild-type isolate was shown to be easily transmissible by whiteflies between cowpea plants but not to blackgram and mugbean, suggesting that the insect vector plays a major role in determining the natural host range of these viruses.
Plant Virology Unit, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi 110012, India.
P
P
John
P N
PN
Sivalingam
Q M I
QM
Haq
N
N
Kumar
A
A
Mishra
R W
RW
Briddon
V G
VG
Malathi
D000818:Animals; D053839:Begomovirus / Q000235:genetics* / Q000302:isolation & purification; D003001:Cloning, Molecular; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics*; D018562:Disease Transmission, Infectious; D004199:Disease Vectors; D007887:Fabaceae / Q000821:virology*; D006430:Hemiptera / Q000821:virology; D007194:India; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology
D004279:DNA, Viral