[Effect of puerarin and ligustrazine on cultured hippocampal neurons injury induced by Abeta25-35].
OBJECTIVE
To investigate the changes of MDA, SOD, LDH of cultured hippocampal neurons injury induced by amyloid-beta protein (Abeta 25-35) and the protective effect of puerarin and ligustrazine.


METHODS
Primary hippocampal neurons were cultured and induced by Abeta 25-35. The concentrations of MDA, SOD and LDH in cultured hippocampal neurons were measured after exposed to Abeta 25-35, puerarin and ligustrazine.


RESULTS
The Alzheimer disease (AD) model was successfully established in cultured hippocampal neurons. AD group has remarkably increased MDA and LDH level, and decreased SOD level, Piracetan group and combined application group of have remarkably decreased MDA and LDH level and increased SOD level, compared with AD group (P < 0.01). Ligustrazine together with puerarin group has remarkably decreased MDA and LDH level and increased SOD level, compared with ligustrazine group and puerarin group (P < 0.05).


CONCLUSIONS
Abeta 25-35 can induce cultured hippocampal neurons injury, combined application of ligustrazine, and puerarin can alleviate the injury.
Zhejiang Chinese Medical University, Institute of Cardio-cerebrovascular Disease, Hangzhou 310053, China.
Yu
Y
Wang
Hai-Tong
HT
Wan
Wei-Min
WM
Yan
Jie-Hong
JH
Yang
Ying
Y
Guo
Yuan-Jiang
YJ
Pan
Jin
J
Han
Qin
Q
Yu
D016229:Amyloid beta-Peptides / Q000494:pharmacology*; D000818:Animals; D002478:Cells, Cultured; D006624:Hippocampus / Q000166:cytology*; D007529:Isoflavones / Q000494:pharmacology*; D009474:Neurons / Q000187:drug effects*; D011719:Pyrazines / Q000494:pharmacology*; D051381:Rats
D016229:Amyloid beta-Peptides; D007529:Isoflavones; D011719:Pyrazines; C017953:tetramethylpyrazine; C033607:puerarin