Genetic mapping of vocalization to a series of increasing acute footshocks using B6.A consomic and B6.D2 congenic mouse strains.
Footshock response is used to study a variety of biological functions in mammals including drug self-administration, learning and memory and nociception. However, the genetics underlying variability in footshock sensitivity are not well understood. In the current studies, a panel of B6.A consomic mouse strains, two B6.D2 genome-tagged mouse lines, and the progenitor strains were screened for footshock sensitivity as measured by audible vocalization. It was found that A/J (A) mice and C57BL/6J (B6) mice with an A Chromosome 1 (Chr 1) were less sensitive to footshock compared to B6 animals. Furthermore, the offspring of Chr 1 consomic mice crossed with B6 mice had vocalization levels that were intermediate to A/J and B6 animals. A F2 mapping panel revealed two significant QTLs for footshock vocalization centered around D1Mit490 and D1Mit206 on Chr 1. The role of these Chr 1 loci in footshock sensitivity was confirmed in B6.D2 genome-tagged mouse lines.
Matthews
Douglas B
DB
Department of Psychology, University of Memphis, Memphis, TN 38152, USA. doug_matthews@baylor.edu
Chesler
Elissa J
EJ
Cook
Melloni N
MN
Cockroft
Jody
J
Philip
Vivek M
VM
Goldowitz
Dan
D
D000818:Animals; D020296:Animals, Congenic / Q000235:genetics*; D002874:Chromosome Mapping; D004597:Electroshock; D051379:Mice; D008805:Mice, Inbred A; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains / Q000235:genetics*; D012684:Sensory Thresholds / Q000502:physiology*; D013045:Species Specificity; D014828:Vocalization, Animal / Q000502:physiology*