p-Coumaric acid, a constituent of Sasa quelpaertensis Nakai, inhibits cellular melanogenesis stimulated by alpha-melanocyte stimulating hormone.
BACKGROUND
Recent study has demonstrated that Sasa quelpaertensis (Korean name, Jeju-Joritdae) extracts inhibit cellular melanogenesis implicating potential use in the control of skin pigmentation.


OBJECTIVE
The purpose of the present study was to elucidate the active constituents of this plant inhibiting melanogenesis and the associated mechanism.


METHODS
The effect of the plant-derived materials on melanin production and/or tyrosinase expression was examined in murine melanoma B16/F10 cells and neonatal human melanocytes.


RESULTS
When tested in melanoma B16/F10 cells treated with the alpha-melanocyte stimulating hormone (alpha-MSH), the aqueous ethanol extract of S. quelpaertensis culm inhibited the cellular melanogenesis more effectively than its leaf extract. A major active compound was isolated from the culm extract by solvent fractionation and column chromatography, and identified to be p-coumaric acid by spectroscopic and chromatographic analyses. The compound (p-coumaric acid) inhibited alpha-MSH-stimulated cellular melanogenesis more effectively than arbutin or other structurally similar compounds including 3-(4-hydroxyphenyl) propionic acid, cinnamic acid and caffeic acid. It also attenuated alpha-MSH-dependent increase of tyrosinase protein. The antimelanogenic effect of p-coumaric acid was also verified in neonatal human melanocytes.


CONCLUSIONS
The present study identified p-coumaric acid as a main constituent of S. quelpaertensis inhibiting cellular melanogenesis. Because of its structural similarity, p-coumaric acid may interfere with l-tyrosine action in the control of tyrosinase expression in response to alpha-MSH.
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Department of Molecular Medicine and Cell and Matrix Research Institute, Kyungpook National University School of Medicine, 101 Dongin-dong 2-ga, Jung-gu, Daegu 700-422, Korea.
Lee
S I
SI
Choi
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Moon
S-W
SW
Boo
Y C
YC
D000818:Animals; D001104:Arbutin / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D003373:Coumaric Acids / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008543:Melanins / Q000096:biosynthesis*; D008544:Melanocytes / Q000187:drug effects / Q000378:metabolism; D008546:Melanoma, Experimental / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D011422:Propionates; D031787:Sasa / Q000737:chemistry*; D014407:Tumor Cells, Cultured; D000521:alpha-MSH / Q000494:pharmacology*
D003373:Coumaric Acids; D008543:Melanins; D010936:Plant Extracts; D011422:Propionates; D000521:alpha-MSH; D001104:Arbutin; C495469:p-coumaric acid