Structural genomics of pathogenic protozoa: an overview.
The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.
Fan
Erkang
E
Department of Biochemistry, University of Washington, Seattle, WA, USA.
Baker
David
D
Fields
Stanley
S
Gelb
Michael H
MH
Buckner
Frederick S
FS
Van Voorhis
Wesley C
WC
Phizicky
Eric
E
Dumont
Mark
M
Mehlin
Christopher
C
Grayhack
Elizabeth
E
Sullivan
Mark
M
Verlinde
Christophe
C
Detitta
George
G
Meldrum
Deirdre R
DR
Merritt
Ethan A
EA
Earnest
Thomas
T
Soltis
Michael
M
Zucker
Frank
F
Myler
Peter J
PJ
Schoenfeld
Lori
L
Kim
David
D
Worthey
Liz
L
Lacount
Doug
D
Vignali
Marissa
M
Li
Jizhen
J
Mondal
Somnath
S
Massey
Archna
A
Carroll
Brian
B
Gulde
Stacey
S
Luft
Joseph
J
Desoto
Larry
L
Holl
Mark
M
Caruthers
Jonathan
J
Bosch
Jürgen
J
Robien
Mark
M
Arakaki
Tracy
T
Holmes
Margaret
M
Le Trong
Isolde
I
Hol
Wim G J
WG
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D023281:Genomics / Q000379:methods*; D010961:Plasmodium / Q000235:genetics*; D015800:Protozoan Proteins / Q000737:chemistry*; D014351:Trypanosomatina / Q000235:genetics*
D015800:Protozoan Proteins