Herpes simplex virus type 2 displays atypical melting-temperature profiles at low viral titers.
Real-time PCR is a powerful tool for the detection and typing of herpes simplex virus (HSV). HSV types 1 and 2 can be distinguished by using the differences in the melting temperatures (T(m)s) of the hybridization probes. The efficacy of T(m) profiling with low DNA concentrations was evaluated in this study.
Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, Halifax, Nova Scotia, Canada.
Jason J
JJ
LeBlanc
Sarah
S
Campbell
Janice
J
Pettipas
Todd F
TF
Hatchette
Ross J
RJ
Davidson
D002555:Cerebrospinal Fluid / Q000821:virology; D004279:DNA, Viral / Q000737:chemistry* / Q000235:genetics*; D005260:Female; D018258:Herpesvirus 2, Human / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006801:Humans; D008046:Lip / Q000821:virology; D008297:Male; D010413:Penis / Q000821:virology; D014059:Tongue / Q000821:virology; D044366:Transition Temperature; D014844:Vulva / Q000821:virology
D004279:DNA, Viral