Influence of Ni(2+) concentration on biohydrogen production.
In this paper, the effect of Ni(2+) concentration ranging from 0 to 50mg/L on fermentative hydrogen production by mixed cultures was investigated in batch test. The results showed that at 35 degrees C and initial pH 7.0, Ni(2+) was able to enhance the hydrogen production rate with increasing Ni(2+) concentration from 0 to 0.2mg/L, and enhance the hydrogen production potential and hydrogen yield with increasing Ni(2+) concentration from 0 to 0.1mg/L. The maximum hydrogen production potential of 288.6 mL and the maximum hydrogen yield of 296.1 mL/g glucose were obtained at the Ni(2+) concentration of 0.1mg/L. In all tests, the major soluble metabolites produced by mixed cultures were ethanol, acetic acid and butyric acid, without propionic acid. Ni(2+) had little effect on the substrate degradation efficiency with increasing concentration from 0 to 50mg/L. Ni(2+) was able to enhance the biomass production yield with increasing Ni(2+) concentration from 0 to 0.1mg/L. The maximum biomass production yield of 232.5mg/g glucose was obtained at the Ni(2+) concentration of 0.1mg/L. In all tests, the final pH after fermentative hydrogen production was lower than the initial pH.
Tsinghua University, Beijing 100084, People's Republic of China. wangjl@tsinghua.edu.cn
Jianlong
J
Wang
Wei
W
Wan
D018533:Biomass; D005285:Fermentation / Q000187:drug effects; D005947:Glucose / Q000378:metabolism; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration / Q000187:drug effects; D009532:Nickel / Q000494:pharmacology*; D012995:Solubility / Q000187:drug effects; D013997:Time Factors
D009532:Nickel; D006859:Hydrogen; D005947:Glucose