Characterization of three new serous epithelial ovarian cancer cell lines.
BACKGROUND
Cell lines constitute a powerful model to study cancer, and here we describe three new epithelial ovarian cancer (EOC) cell lines derived from poorly differentiated serous solid tumors (TOV-1946, and TOV-2223G), as well as the matched ascites for one case (OV-1946).


METHODS
In addition to growth parameters, the cell lines were characterized for anchorage independent growth, migration and invasion potential, ability to form spheroids and xenografts in SCID mice.


RESULTS
While all cell lines were capable of anchorage independent growth, only the TOV-1946 and OV-1946 cell lines were able to form spheroid and produce tumors. Profiling of keratins, p53 and Her2 protein expression was assessed by immunohistochemistry and western blot analyses. Somatic TP53 mutations were found in all cell lines, with TOV-1946 and OV-1946 harboring the same mutation, and none harbored the commonly observed somatic mutations in BRAF, KRAS or germline BRCA1/2 mutations found to recur in the French Canadian population. Conventional cytogenetics and spectral karyotype (SKY) analyses revealed complex karyotypes often observed in ovarian disease.


CONCLUSIONS
This is the first report of the establishment of matched EOC cell lines derived from both solid tumor and ascites of the same patient.
Ouellet
Véronique
V
Centre de recherche du Centre hospitalier de l'Université de Montréal/Institut du cancer de Montréal, Montreal, Canada. ouelletv@yahoo.ca
Zietarska
Magdalena
M
Portelance
Lise
L
Lafontaine
Julie
J
Madore
Jason
J
Puiffe
Marie-Line
ML
Arcand
Suzanna L
SL
Shen
Zhen
Z
Hébert
Josée
J
Tonin
Patricia N
PN
Provencher
Diane M
DM
Mes-Masson
Anne-Marie
AM
D000818:Animals; D001201:Ascites / Q000473:pathology; D048708:Cell Growth Processes / Q000502:physiology; D045744:Cell Line, Tumor; D018284:Cystadenocarcinoma, Serous / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004847:Epithelial Cells / Q000473:pathology; D005260:Female; D006801:Humans; D007621:Karyotyping; D007633:Keratins / Q000096:biosynthesis; D051379:Mice; D016513:Mice, SCID; D009154:Mutation; D010051:Ovarian Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000096:biosynthesis; D014183:Transplantation, Heterologous; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis
D007633:Keratins; D018719:Erb-b2 Receptor Tyrosine Kinases; D016159:Tumor Suppressor Protein p53; C495901:TP53 protein, human