Ionotropic GABA receptor expression in the lung during development.
Cl(-) transport is essential for lung development. Because gamma-aminobutyric acid (GABA) receptors allow the flow of negatively-charged Cl(-) ions across the cell membrane, we hypothesized that the expression of ionotropic GABA receptors are regulated in the lungs during development. We identified 17 GABA receptor subunits in the lungs by real-time PCR. These subunits were categorized into four groups: Group 1 had high mRNA expression during fetal stages and low in adults; Group 2 had steady expression to adult stages with a slight up-regulation at birth; Group 3 showed an increasing expression from fetal to adult lungs; and Group 4 displayed irregular mRNA fluctuations. The protein levels of selected subunits were also determined by Western blots and some subunits had protein levels that corresponded to mRNA levels. Further studied subunits were primarily localized in epithelial cells in the developing lung with differential mRNA expression between isolated cells and whole lung tissues. Our results add to the knowledge of GABA receptor expression in the lung during development.
Department of Physiological Sciences, Oklahoma State University, 264 McElroy Hall, Stillwater, OK 74078, USA.
Nili
N
Jin
Yujie
Y
Guo
Peng
P
Sun
Anna
A
Bell
Narendranath Reddy
NR
Chintagari
Manoj
M
Bhaskaran
Kimberly
K
Rains
Pradyumna
P
Baviskar
Zhongming
Z
Chen
Tingting
T
Weng
Lin
L
Liu
D000818:Animals; D004847:Epithelial Cells / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D018507:Gene Expression Regulation, Developmental; D008168:Lung / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D008297:Male; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018079:Receptors, GABA / Q000032:analysis / Q000235:genetics / Q000378:metabolism*
D021122:Protein Subunits; D012333:RNA, Messenger; D018079:Receptors, GABA