Brazilin and the extract from Caesalpinia sappan L. protect oxidative injury through the expression of heme oxygenase-1.
In this study, we examined the protective effects of Caesalpinia sappan L. and its major component, brazilin, against tert-butylhydroperoxide (t-BHP)-induced cell death in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells. We found that the extract of C. sappan L. and brazilin induced antioxidant response element (ARE)-luciferase activity and heme oxygenase-1 (HO-1) expression in a concentration-dependent manner. The inductive effect of brazilin was more potent than the extract of C. sappan L. and the expression of HO-1 reached a peak at 12 h after brazilin treatment. The extract and brazilin protected the cells against t-BHP-induced cell death. Their protective effects were abrogated by zinc protoporphyrin IX (ZnPP IX), a HO inhibitor. These results demonstrate that the extract of C. sappan L. and brazilin induce the expression of HO-1 and the enzyme diminishes t-BHP-induced cell death in HEI-OC1 cells.
Choi
Byung-Min
BM
Vestibulocochlear Research Center and Department of Biochemistry, School of Medicine, Wonkwang University, Chonbuk, Republic of Korea.
Lee
Ju-A
JA
Gao
Shang Shang
SS
Eun
Sang Yong
SY
Kim
Young-Sup
YS
Ryu
Shi-Yong
SY
Choi
Yeon-Hee
YH
Park
Raekil
R
Kwon
Dae Young
DY
Kim
Bok-Ryang
BR
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001578:Benzopyrans / Q000737:chemistry / Q000494:pharmacology*; D015153:Blotting, Western; D029911:Caesalpinia / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000378:metabolism*; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D015394:Molecular Structure; D009925:Organ of Corti / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011524:Protoporphyrins / Q000494:pharmacology; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020218:Response Elements / Q000235:genetics; D014162:Transfection; D020122:tert-Butylhydroperoxide / Q000494:pharmacology
D000975:Antioxidants; D001578:Benzopyrans; D010936:Plant Extracts; D011524:Protoporphyrins; D011993:Recombinant Fusion Proteins; C017803:zinc protoporphyrin; D020122:tert-Butylhydroperoxide; D008156:Luciferases; D051547:Heme Oxygenase-1; C044362:brazilin