[Effect of nordy on the function of endothelial progenitor cells from human umbilical cord blood induced by vascular endothelial growth factor].
This study is to investigate whether the synthesized chiral compound Nordy has influence on the function of endothelial progenitor cells (EPCs) from human umbilical cord blood induced by vascular endothelial growth factor (VEGF). EPCs were isolated from human umbilical cord blood by density gradient centrifugation. After cultured for 7 -10 days, EPCs were prepared for detecting effect of Nordy on proliferation, migration and tubule-forming activity in Matrigel induced by VEGF. Incubation of EPCs with 100 micromol L(-1) Nordy for 24 h initially inhibited the proliferative capacity of EPCs induced by VEGF (P <0.05). Moreover, 25 -50 micromol L(-1) Nordy also exhibited inhibitory effect at 48 -72 h. In addition, 25 - 100 micromol L(-1) Nordy impaired EPCs migratory and tubule-forming capacity in vitro (P < 0.05). Nordy could inhibit in EPCs the functions of proliferation, migration and tubulogenesis induced by VEGF in vitro, which might be a possible mechanism of its anti-EPCs effects.
Institute of Pathology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Hua-Rong
HR
Zhang
Cheng-Ping
CP
Xu
Fei-Lan
FL
Chen
Xiu-Wu
XW
Bian
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000166:cytology; D005312:Fetal Blood / Q000166:cytology; D006801:Humans; D009637:Masoprocol / Q000031:analogs & derivatives* / Q000494:pharmacology; D018919:Neovascularization, Physiologic / Q000187:drug effects*; D013234:Stem Cells / Q000166:cytology*; D042461:Vascular Endothelial Growth Factor A / Q000037:antagonists & inhibitors
D000970:Antineoplastic Agents; D042461:Vascular Endothelial Growth Factor A; C510003:nordy; D009637:Masoprocol