Sphingobacterium siyangense sp. nov., isolated from farm soil.
The taxonomic position of a novel Gram-negative strain, designated SY1(T), isolated from a farm-soil sample obtained from Jiangsu Province, PR China, was characterized by using a polyphasic approach. The cells were non-motile, non-spore-forming rods. The organism grew optimally at 30-37 degrees C and at pH 6.0-8.0. Based on 16S rRNA gene sequence analysis, strain SY1(T) is a member of the genus Sphingobacterium; Sphingobacterium multivorum JCM 21156(T) was the nearest relative (98.5 % sequence similarity). The predominant fatty acids of strain SY1(T) were iso-C(15 : 0) (32.9 %), C(16 : 0) (10.9 %) and summed feature 3 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c; 24.1 %). The DNA G+C content was 38.5 mol%. The low level of DNA-DNA relatedness (2.2 %) to S. multivorum JCM 21156(T) in combination with differential morphological and biochemical properties demonstrated that strain SY1(T) (=KCTC 22131(T)=CGMCC 1.6855(T)) should be classified as representing a novel species of the genus Sphingobacterium for which the name Sphingobacterium siyangense sp. nov. is proposed.
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Life Sciences College of Nanjing Agricultural University, Nanjing, Jiangsu 210095, PR China.
Rui
R
Liu
Huan
H
Liu
Chong-Xing
CX
Zhang
Shou-Yun
SY
Yang
Xiu-Hong
XH
Liu
Ke-Yun
KY
Zhang
Ren
R
Lai
D000383:Agriculture; D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D045249:Sphingobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S