Proteomic analysis of early germs with high-oil and normal inbred lines in maize.
High-oil maize as a product of long-term selection provides a unique resource for functional genomics. In this study, the abundant soluble proteins of early developing germs from high-oil and normal lines of maize were compared using two-dimensional gel electrophoresis (2-DGE) in combination with mass spectrometry (MS). More than 1100 protein spots were detected on electrophoresis maps of both high-oil and normal lines by using silver staining method. A total of 83 protein spots showed significant differential expression (>two-fold change; t-test: P < 0.05) between high-oil and normal inbred lines. Twenty-seven protein spots including 25 non-redundant proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Functional categorization of these proteins was carbohydrate metabolism, cytoskeleton, protein metabolism, stress response, and lipid metabolism. Three such proteins involved in lipid metabolism, namely putative enoyl-ACP reductase (ENR), putative stearoyl-ACP desaturase (SAD) and putative acetyl-CoA C-acyltransferase (ACA), had more abundant expressions in high-oil lines than in normal. At the mRNA expression level, SAD, ENR and ACA were expressed at significantly higher levels in high-oil lines than in normal. The results demonstrated that high expressions of SAD, ENR and ACA might be associated to increasing oil concentration in high-oil maize. This study represents the first proteomic analysis of high-oil maize and contributes to a better understanding of the molecular basis of oil accumulation in high-oil maize.
National Maize Improvement Center of China, China Agricultural University, Beijing 100094, China.
Zhanji
Z
Liu
Xiaohong
X
Yang
Yang
Y
Fu
Yirong
Y
Zhang
Jianbin
J
Yan
Tongming
T
Song
T
T
Rocheford
Jiansheng
J
Li
D015180:Electrophoresis, Gel, Two-Dimensional; D005227:Fatty Acids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018525:Germination; D010938:Plant Oils / Q000378:metabolism*; D040901:Proteomics; D013997:Time Factors; D014158:Transcription, Genetic / Q000235:genetics; D003313:Zea mays / Q000254:growth & development* / Q000378:metabolism*
D005227:Fatty Acids; D010938:Plant Oils