Validation of a low cost computer-based method for quantification of immunohistochemistry-stained sections.
The aim of the present study was to determine if the Alpha DigiDoc RT system would be an effective method of quantifying immunohistochemical staining as compared with a manual counting method, which is considered the gold standard. Two readers were used to count 31 samples by both methods. The results obtained using the Bland-Altman for concordance deemed no statistical difference between the 2 methods. Thus, the Alpha DigiDoc RT system is an effective, low cost method to quantify immunohistochemical data.
Department of Infectious Diseases and Microbiology, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD, USA.
Jill D
JD
Montgomery
Heather R
HR
Hensler
Lisa P
LP
Jacobson
Frank J
FJ
Jenkins
D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D003201:Computers / Q000191:economics; D006801:Humans; D007150:Immunohistochemistry / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D008297:Male; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000473:pathology; D013194:Staining and Labeling / Q000191:economics / Q000295:instrumentation / Q000379:methods*
D000951:Antigens, Neoplasm