Comparison of the antioxidant activity of albumin from various animal species.
We measured the antioxidant activity of human, rat, bovine, rabbit, and guinea pig albumins against the superoxide, hydroxyl, and 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radicals. The albumins of different animal species did not differ in antioxidant activity against superoxide. Human and rat albumins exhibited antioxidant activity against hydroxyl radicals, but bovine, rabbit, and guinea pig albumins showed weaker antioxidant activity than human and rat albumins. Human, rat, rabbit, and guinea pig albumins, but not bovine albumin, exhibited strong antioxidant activity against DPPH radicals. Human and rat albumins with strong antioxidant activity against hydroxyl radicals contained methionine-123 in domain 1, but bovine, rabbit, and guinea pig albumins did not. Rat, rabbit, and guinea pig albumins with strong antioxidant activity against DPPH radicals had methionine-264 in domain 2. Human albumin did not have methionine-264, but methionine-298 and methionine-329 in domain 2. Bovine albumin, with the weakest antioxidant activity against DPPH radicals, contained no methionine residues in domain 2. These results suggest that methionine residues in domain 1 or 2 influence the antioxidant activity of albumin.
Department of Hematological Informatics, Kitasato University School of Allied Health Science, 1-15-1 Kitasato, Sagamihara-shi, Kanagawa 228-8555, Japan. okazaki@cc.ahs.kitasato-u.ac.jp
Toshio
T
Okazaki
Noriyuki
N
Okudaira
Naohito
N
Ishii
Hiroshi
H
Yotsuyanagi
Toshiaki
T
Nagai
Shougo
S
Tokudome
Takahiro
T
Fujioka
Shinichiro
S
Takahashi
D000418:Albumins / Q000737:chemistry / Q000494:pharmacology*; D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D001713:Biphenyl Compounds; D002417:Cattle; D006168:Guinea Pigs; D006801:Humans; D017665:Hydroxyl Radical; D008969:Molecular Sequence Data; D010851:Picrates; D011817:Rabbits; D051381:Rats; D013045:Species Specificity; D013481:Superoxides
D000418:Albumins; D000975:Antioxidants; D001713:Biphenyl Compounds; D010851:Picrates; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl