Analysis of heartsease (Viola tricolor L.) flavonoid glycosides by micro-liquid chromatography coupled to multistage mass spectrometry.
Micro-liquid chromatography (microLC) in conjunction with multistage mass spectrometry (MSn) was introduced to study several major heartsease flavonoid glycosides. High-resolution microLC separation was achieved by using a monolithic poly(p-methylstyrene-co-1,2-bis(p-vinylphenyl)ethane) column under reversed-phase conditions. The MS/MS and MS3 analysis of the flavonoid components of interest provided data about their glycosylation type and position, nature of their aglycones, and the structure/linkage information of their glycan moieties. With our microLC-MSn approach, four flavonol O-glycosides, nine flavone-C-glycosides, and three flavone C,O-glycosides were characterized in heartsease methanol extract. All of these glycoconjugates were found to be the derivatives of six aglycones: apigenin, chrysoeriol, isorhamnetin, kaempferol, luteolin, and quercetin.
Horváth Laboratory of Bioseparation Sciences, Institute of Analytical and Radiochemistry, University of Innsbruck, Innrain 52A, A-6020 Innsbruck, Austria.
Viktoria
V
Vukics
Thomas
T
Ringer
Agnes
A
Kery
Guenther K
GK
Bonn
Andras
A
Guttman
D047310:Apigenin / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D047309:Flavones / Q000737:chemistry; D005419:Flavonoids / Q000737:chemistry* / Q000302:isolation & purification; D044948:Flavonols / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D044949:Kaempferols / Q000737:chemistry; D047311:Luteolin / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D011794:Quercetin / Q000737:chemistry / Q000031:analogs & derivatives; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D032413:Viola / Q000737:chemistry*
D047309:Flavones; D005419:Flavonoids; D044948:Flavonols; D006027:Glycosides; D044949:Kaempferols; C047368:3-methylquercetin; C006552:kaempferol; D047310:Apigenin; D011794:Quercetin; D047311:Luteolin; C007054:chrysoeriol