Detection of intact influenza viruses using biotinylated biantennary S-sialosides.
We report the modular synthesis of robust, biotinylated biantennary sialylglycoconjugates and their ability to differentiate between two type A influenza strains. This is the first demonstration of glycoconjugate-based discriminatory capture and detection of two strains of intact influenza virus, in the presence of the innate enzymatic activity of viral neuraminidases. We also demonstrate a "carboassay" using glycoconjugates as capture and reporter elements, which therefore, does not require antibodies. The capture of intact influenza viruses is of potential benefit for clinical diagnostics.
Kale
Ramesh R
RR
Chemical and Biosensors group, 805 Crosley, Department of Chemistry, University of Cincinnati, Cincinnati, Ohio 45221, USA.
Mukundan
Harshini
H
Price
Dominique N
DN
Harris
J Foster
JF
Lewallen
Daniel M
DM
Swanson
Basil I
BI
Schmidt
Jurgen G
JG
Iyer
Suri S
SS
D019921:Biotinylation; D006001:Glycoconjugates; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D012794:Sialic Acids; D013057:Spectrum Analysis
D006001:Glycoconjugates; D012794:Sialic Acids