Transcriptional regulation of ketone body-utilizing enzyme, acetoacetyl-CoA synthetase, by C/EBPalpha during adipocyte differentiation.
Acetoacetyl-CoA synthetase (AACS), an essential enzyme for the synthesis of fatty acid and cholesterol from ketone bodies, was found to be highly expressed in mouse adipose tissue, and GC box and C/EBPs motif were crucial for AACS promoter activity in 3T3-L1 adipocytes. Moreover, we found that AACS promoter activity was controlled mainly by C/EBPalpha during adipogenesis.
Department of Health Chemistry, Hoshi University, Shinagawa-ku, Tokyo 142-8501, Japan. s-hasegawa@hoshi.ac.jp
Shinya
S
Hasegawa
Masahiro
M
Yamasaki
Tasuku
T
Inage
Noriko
N
Takahashi
Tetsuya
T
Fukui
D041721:3T3-L1 Cells; D000106:Acetate-CoA Ligase / Q000235:genetics*; D017667:Adipocytes / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*; D050156:Adipogenesis / Q000235:genetics / Q000502:physiology; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites / Q000235:genetics; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000378:metabolism*; D002454:Cell Differentiation; D047369:Chromatin Immunoprecipitation; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D024202:Electrophoretic Mobility Shift Assay; D020869:Gene Expression Profiling; D007657:Ketone Bodies / Q000378:metabolism*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic
D022763:CCAAT-Enhancer-Binding Protein-alpha; D017931:DNA Primers; D018076:DNA, Complementary; D007657:Ketone Bodies; D012333:RNA, Messenger; D000106:Acetate-CoA Ligase