Effects of docosahexaenoic acid on some megakaryocytic cell gene expression of some enzymes controlling prostanoid synthesis.
Beneficial effects of docosahexaenoic acid (DHA) intake in the prevention of cardiovascular diseases are known, and platelets play a crucial role in cardiovascular complications. However, high doses of DHA may increase lipid peroxidation and induce deleterious effects, notably in platelets. This led us to investigate the effect of DHA on gene expression of some enzymes controlling redox status and prostanoid formation in human megakaryoblastic cells (MEG-01 cell line). MEG-01 cells were incubated in presence of DHA (10 and 100 micromol/L) for 6h. DHA enrichment up-regulated glutathione peroxidase-1 and thromboxane synthase mRNA. DHA increased gene catalase expression and up-regulated PPAR beta/delta and PPAR gamma mRNA in presence of high concentration of DHA. In conclusion, our results support an antioxidant mechanism of DHA. The effects of DHA on cellular redox status could, with others, provide an explanation for the beneficial influence of low consumption of DHA on cardiovascular events.
Guillot
Nicolas
N
Université de Lyon, INSERM, U.870, INSA-Lyon, (RMND-IMBL), Univ-Lyon 1, Bât Louis Pasteur, 20 Ave. A. Einstein, F-69621 Villeurbanne Cedex, France; INRA, UMR 1235, F-69600 Oullins, France.
Debard
Cyrille
C
Calzada
Catherine
C
Vidal
Hubert
H
Lagarde
Michel
M
Véricel
Evelyne
E
D016718:Arachidonic Acid / Q000378:metabolism; D002460:Cell Line; D004281:Docosahexaenoic Acids / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis; D015870:Gene Expression / Q000187:drug effects*; D005979:Glutathione Peroxidase / Q000235:genetics*; D006801:Humans; D008315:Malondialdehyde; D008533:Megakaryocytes / Q000187:drug effects / Q000201:enzymology; D010084:Oxidation-Reduction / Q000187:drug effects; D047494:PPAR delta / Q000235:genetics; D048068:PPAR-beta / Q000235:genetics; D010743:Phospholipids / Q000737:chemistry; D011451:Prostaglandin-Endoperoxide Synthases / Q000235:genetics; D011453:Prostaglandins / Q000096:biosynthesis* / Q000235:genetics; D000094765:Glutathione Peroxidase GPX1
D005227:Fatty Acids; D047494:PPAR delta; D048068:PPAR-beta; D010743:Phospholipids; D011453:Prostaglandins; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D011451:Prostaglandin-Endoperoxide Synthases; D000094765:Glutathione Peroxidase GPX1; C000722067:GPX1 protein, human