Effect of taurine and melatonin in the culture medium on buffalo in vitro embryo development.
This study was carried out to investigate the effect of supplementing culture medium with different concentrations of taurine and melatonin, on buffalo oocyte in vitro meiotic maturation and embryo development. In experiment 1, oocytes were matured in vitro and the cleaved embryos were cultured in the same following seven culture medium; (i) control (TCM 199 + 10% SS); (ii) control + 0.5 mM taurine; (iii) control + 1 mM taurine; (iv) control + 3 mM taurine; (v) control + 5 microM melatonin; (vi) control + 10 microM melatonin and (vii) control + 50 microM melatonin. In experiment 2, based on the results of experiment 1, to examine the synergistic effect of antioxidants, the oocytes were matured in culture medium (TCM199 + 10% SS), supplemented with both taurine at 1 mM and melatonin at 10 microM concentration and the cleaved embryos were cultured in the same medium. Supplementation of taurine at 1 mM concentration in the culture medium resulted in a higher (p < 0.05) transferable embryo (TE) yield when compared with control (20.6% vs 14.1%). Supplementation of melatonin at 10 and 50 microM concentration in the culture medium resulted in a higher (p < 0.05) meiotic maturation rate (90.3% and 88.8% respectively) and TE yield (28.4% and 27.2% respectively), than the other treatments. In experiment 2, the TE yield did not improve by supplementing the culture medium with both taurine and melatonin, when compared with melatonin alone. In conclusion, the results of this study demonstrated that, enriching the culture medium with taurine and melatonin, improves in vitro embryo production efficiency in buffaloes. In particular, a high TE yield was obtained by enriching the culture medium with 10 microM melatonin.
Manjunatha
B M
BM
Department of Animal Reproduction, Gynaecology and Obstetrics, Veterinary College, Karnataka Veterinary, Animal and Fishery Sciences University (KVAFSU), India. drmanjunathvet@gmail.com
Devaraj
M
M
Gupta
P S P
PS
Ravindra
J P
JP
Nandi
S
S
D000818:Animals; D002020:Buffaloes / Q000196:embryology*; D003470:Culture Media; D046149:Embryo Culture Techniques / Q000379:methods / Q000662:veterinary*; D047108:Embryonic Development / Q000187:drug effects*; D005307:Fertilization in Vitro / Q000662:veterinary; D008540:Meiosis / Q000187:drug effects; D008550:Melatonin / Q000494:pharmacology*; D009865:Oocytes / Q000187:drug effects; D013654:Taurine / Q000494:pharmacology*
D003470:Culture Media; D013654:Taurine; D008550:Melatonin