Lipocalin-like prostaglandin D synthase in subretinal fluid of detached retinas in humans.
BACKGROUND
The presence of lipocalin-like prostaglandin D synthase (L-PGDS), a dominantly brain derived protein in the cerebrospinal fluid (CSF) with a low concentration in serum, in the subretinal fluid (SRF) of detached retinas, has not been reported. L-PGDS has been demonstrated in the ciliary body and the retinal pigment epithelium in rats.


METHODS
SRF was sampled during surgery (scleral buckling) of rhegmatogenous retinal detachments in 20 eyes. Biochemical and nephelometric analysis for L-PGDS concentration were performed. Albumin concentrations were determined in the same samples.


RESULTS
L-PGDS concentrations in SRF (mean 18.9 mg/L +/- 14.8 mg/L) were markedly higher than the normal L-PGDS concentration in serum (<0.55 mg/L) and appears to decrease with the duration of the retinal detachment (P = 0.0064).


CONCLUSIONS
As the subretinal space is not accessible in attached retinas there are no data on normal levels of L-PGDS in the subretinal space. The L-PGDS concentrations measured in the SRF of detached retinas are on average 34 times higher than normal L-PGDS serum concentration and about in the range of normal L-PGDS CSF concentration (15.3 mg/L). As the concentrations of L-PGDS exceed normal serum levels, a choriocapillary transudation is highly improbable as the source of L-PGDS in SRF of detached retinas.
Department of Ophthalmology, Kantonsspital Aarau, Switzerland.
Gregor P
GP
Jaggi
Josef
J
Flammer
Andreas R
AR
Huber
Hanspeter E
HE
Killer
D000368:Aged; D000369:Aged, 80 and over; D001826:Body Fluids / Q000201:enzymology*; D005122:Exudates and Transudates / Q000201:enzymology; D005260:Female; D006801:Humans; D019746:Intramolecular Oxidoreductases / Q000378:metabolism*; D054834:Lipocalins / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009391:Nephelometry and Turbidimetry; D012163:Retinal Detachment / Q000201:enzymology* / Q000601:surgery; D012591:Scleral Buckling; D013997:Time Factors
D054834:Lipocalins; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase