Suppression of mutagens-induced SOS response by phytoncide solution using Salmonella typhimurium TA1535/pSK1002 umu test.
Four types of phytoncide solution (A-Type, AB-Type, D-Type and G-Type) were evaluated as antimutagenic agents with suppressive effects on the SOS-inducing activity of the mutagen 2-(2-furyl)-3-(5-nitro-2-furyl) acrylamide (furylfuramide) using Salmonella typhimurium TA1535/pSK1002 umu test. The A-Type, AB-Type, D-Type and G-Type of phytoncide solution suppressed the SOS-inducing activity on furylfuramide at a concentration of 100 microg/mL by 86.1%, 74.7%, 69.5% and 55.4%, respectively, and the ID(50) (50% inhibitory dose) values were 9.0 microg/mL, 22.5 microg/mL, 36.0 microg/mL and 72.8 microg/mL. They also showed the suppression of SOS-inducing activity against other chemical mutagens, such as 4-nitroquinolin 1-oxide (4NQO) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), which do not require liver metabolizing enzymes, and against 2-aminoanthracene (2AA) and 3-amino-1,4-dimethyl-5H-pyrido[4,3-b]indole (Trp-P-1), which require these enzymes, and against UV irradiation, which is a well known physical mutagen. In the search for the component-activity relationship, the A-Type of phutoncide solution suppressed the SOS-inducing activity greater than the other types of phutoncide solution for furylfuramide, 4NQO and MNNG. However, in case of 2AA and Trp-P-1, the D-Type of phytoncide solution was most effective in suppressing the SOS-inducing activity in the umu test. From these results, the four types of phytoncide solutions showed the suppressive effect of SOS-inducing activity against chemical and physical mutagens.
Hisama
Masayoshi
M
Central Research Center, Toyo Beauty Co., Ltd., Higashinakamoto, Osaka, Japan. hisama@toyobeauty.jp
Matsuda
Sanae
S
Tanaka
Tomomi
T
Shibayama
Hiroharu
H
Nomura
Masato
M
Iwaki
Masahiro
M
D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D016587:Antimutagenic Agents / Q000494:pharmacology*; D002243:Carbolines / Q000633:toxicity; D005668:Furylfuramide / Q000633:toxicity; D008401:Gas Chromatography-Mass Spectrometry; D008769:Methylnitronitrosoguanidine / Q000633:toxicity; D009152:Mutagenicity Tests; D009153:Mutagens / Q000633:toxicity*; D013014:SOS Response, Genetics / Q000187:drug effects* / Q000528:radiation effects; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000528:radiation effects; D012996:Solutions; D014466:Ultraviolet Rays
D016587:Antimutagenic Agents; D002243:Carbolines; D009153:Mutagens; D012996:Solutions; D005668:Furylfuramide; D008769:Methylnitronitrosoguanidine; D015112:4-Nitroquinoline-1-oxide; C019132:3-amino-1,4-dimethyl-5H-pyrido(4,3-b)indole