Amalgamation of Chlamydia pneumoniae inclusions with lipid droplets in foam cells in human atherosclerotic plaque.
Chlamydia pneumoniae (Chlamydophila pneumoniae) infect macrophages and accelerates foam cell formation in in vitro experiments, but whether this might occur in human atherosclerosis is unknown. In the present study, we examined 17 carotid artery segments, obtained by endarterectomy, in which the presence of C. pneumoniae was confirmed by both polymerase chain reaction and immunohistochemistry. Electron microscopy demonstrated the presence of structures with the appearance of elementary, reticulate and aberrant bodies of C. pneumoniae in the cytoplasm of macrophage foam cells. The volume of the cytoplasm that was free from vacuoles and lipid droplets in C. pneumoniae-infected foam cells was dramatically reduced, and a phenomenon of the amalgamation of C. pneumoniae inclusions with lipid droplets was detected. Double immunohistochemistry showed that C. pneumoniae-infected foam cells contained a large number of oxidized low-density lipoproteins. The observations provide support to the hypothesis that C. pneumoniae could affect foam cell formation in human atherosclerosis.
Bobryshev
Yuri V
YV
Faculty of Medicine, University of New South Wales, Kensington, NSW, Australia. y.bobryshev@unsw.edu.au
Killingsworth
Murray C
MC
Tran
Dihn
D
Lord
Reginald
R
D000368:Aged; D002340:Carotid Artery Diseases / Q000382:microbiology / Q000473:pathology; D016893:Carotid Stenosis / Q000382:microbiology* / Q000473:pathology; D023521:Chlamydophila Infections / Q000382:microbiology / Q000473:pathology; D016993:Chlamydophila pneumoniae / Q000472:pathogenicity*; D005260:Female; D005487:Foam Cells / Q000382:microbiology* / Q000473:pathology / Q000648:ultrastructure; D006801:Humans; D002479:Inclusion Bodies / Q000382:microbiology* / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D014617:Vacuoles / Q000648:ultrastructure