Enhanced resistance against systemic Candida albicans infection in mice treated with C. albicans DNA.
In this study, double-stranded Candida albicans DNA was administered in systemic C. albicans infection in at dose of 20 microg per mouse at 4, 5 and 6 weeks of age. The level of IL-12 in serum was elevated as a result of yeast DNA treatment and correlated with lower mortality and decreased kidney and liver injury. Macrophage activation was demonstrated by an increase of nitric oxide (NO) and IL-12 production. These effects were Janus activation kinases (JAK)/signal transducer and activator of transcription (STAT) dependent as they were inhibited by selective JAK inhibitor tyrphostin AG-490. DNA influenced adaptive immune response through elevation of anti-Candida IgG antibody production in systemic C. albicans infection. Thus, C. albicans DNA augmented innate and adaptive immune responses against the pathogen.
Dimitrova
Petya
P
Department of Immunology, Institute of Microbiology, Sofia, Bulgaria.
Yordanov
Martin
M
Danova
Svetla
S
Ivanovska
Nina
N
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D002176:Candida albicans / Q000235:genetics / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology* / Q000628:therapy*; D015169:Colony Count, Microbial; D004271:DNA, Fungal / Q000008:administration & dosage / Q000627:therapeutic use*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D053612:Janus Kinases / Q000037:antagonists & inhibitors / Q000276:immunology; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D050791:STAT Transcription Factors / Q000276:immunology; D016019:Survival Analysis; D020032:Tyrphostins / Q000494:pharmacology
D000908:Antibodies, Fungal; D004271:DNA, Fungal; D004791:Enzyme Inhibitors; D007074:Immunoglobulin G; D050791:STAT Transcription Factors; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D018664:Interleukin-12; D009569:Nitric Oxide; D053612:Janus Kinases