Effects of selective iNOS inhibition in sepsis: evaluation of lung tissue damage and blood gases.
NO is an important mediator in the generalized inflammatory response of the body during sepsis and septic shock. We investigated the possible effects of L-arginine and aminoguanidine on plasma NO levels and the interaction between NO levels and lung tissue damage and blood gases in sepsis. Fifty Wistar male rats were used in this study and divided into five groups: group 1, sham group; group 2, CLP (sepsis); group 3, CLP + 10 mg/kg L-arginine administration; group 4, CLP +15 mg/kg aminoguanidine administration; group 5: CLP + L-arginine + aminoguanidine given in similar doses. Sepsis was induced by cecal ligation and puncture (CLP) method. Drugs were administered at postoperative hours 4 and 12. The levels in the aminoguanidine and aminoguanidine + L-arginine groups were similar to the sham group. Lung tissue damage in the sepsis and L-arginine groups was more severe than the other groups.
Department of Thoracic Surgery, Selcuk University, Faculty of Medicine, Konya, Turkey.
Sami
S
Ceran
Mehmet
M
Erikoglu
Mustafa
M
Sahin
Güven Sadi
GS
Sunam
Murat
M
Gölcük
Hatice
H
Pasaoğlu
Fatih
F
Avsar
Sema
S
Hücümenoglu
D000818:Animals; D001120:Arginine / Q000494:pharmacology; D001784:Blood Gas Analysis / Q000706:statistics & numerical data; D004195:Disease Models, Animal; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006146:Guanidines / Q000494:pharmacology; D007958:Leukocyte Count; D008168:Lung / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009569:Nitric Oxide / Q000097:blood / Q000502:physiology*; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors* / Q000502:physiology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000097:blood / Q000188:drug therapy*; D016896:Treatment Outcome
D004791:Enzyme Inhibitors; D006146:Guanidines; D009569:Nitric Oxide; D001120:Arginine; D052247:Nitric Oxide Synthase Type II; C004479:pimagedine