Mesoporous silica templated biolabels with releasable fluorophores for immunoassays.
A novel class of fluorescent immuno-biolabeling systems with extremely high F/P ratio (approximately 1000-6000) were prepared by combining the template method and layer-by-layer (LbL) technique. Labels were constructed by loading organic dye fluorescein diacetate (FDA) molecules onto hollow periodic mesoporous organosilica (H-PMO) particles followed by ployelectrolyte encapsulation and antibody attachment. The labeling systems were stimuli-responsive to the addition of concentrated NaOH with the loaded dye molecules being released and detected in a well-controlled manner. When applied in sandwich immunoassays, results indicated that the biolabels were immuno-active and generated an optimal signal that was approximately 50 times higher than the conventional dye labelled antibody system.
Cai
Wenyi
W
ARC Centre of Excellence for Functional Nanomaterials, The University of Queensland, St. Lucia, 4072, Australia.
Gentle
Ian R
IR
Lu
Gao Qing
GQ
Zhu
Jun-Jie
JJ
Yu
Aimin
A
D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D004573:Electrolytes; D005452:Fluoresceins / Q000032:analysis* / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide; D013499:Surface Properties; D013696:Temperature
D000906:Antibodies; D004573:Electrolytes; D005452:Fluoresceins; D012822:Silicon Dioxide; C018506:diacetylfluorescein