A novel variant in the platelet endothelial aggregation receptor-1 gene is associated with increased platelet aggregability.
OBJECTIVE
Platelet endothelial aggregation receptor-1 (PEAR1) is a recently identified platelet transmembrane protein that becomes activated by platelet contact. We looked for novel genetic variants in PEAR1 and studied their association with agonist-induced native platelet aggregation and with the inhibitory effect of aspirin on platelets.


RESULTS
We genotyped PEAR1 for 10 single nucleotide polymorphisms (SNPs), selected for optimal gene coverage at a density of 4 kb, in 1486 apparently healthy individuals from two generations of families with premature CAD. Subjects had a mean age of 45 years; 62% were white and 38% black. Platelet aggregation to collagen, epinephrine, and ADP was measured in platelet rich plasma, at baseline and after 2 weeks of aspirin (ASA, 81 mg/d), and genotype-phenotype associations were examined separately by ethnicity using multivariable generalized linear models adjusted for covariates. The C allele of SNP rs2768759 [A/C], located in the promoter region of the gene, was common in whites and uncommon in blacks (allele frequency 70.2% versus 17.7%). The C allele was generally associated in both ethnic groups with increased aggregation of native platelets to each agonist. After ASA, the associations were stronger and more consistent and remained significant when post-ASA aggregation was adjusted for baseline aggregation, consistent with a relationship between the C allele and reduced platelet responsiveness to ASA. The PEAR1 SNP explained up to 6.9% of the locus specific genetic variance in blacks and up to 2.5% of the genetic variance in whites after ASA.


CONCLUSIONS
PEAR1 appears to play an important role in agonist-induced platelet aggregation and in the response to ASA in both whites and blacks.
Departments of Medicine and Anesthesiology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA.
J Enrique
JE
Herrera-Galeano
Diane M
DM
Becker
Alexander F
AF
Wilson
Lisa R
LR
Yanek
Paul
P
Bray
Dhananjay
D
Vaidya
Nauder
N
Faraday
Lewis C
LC
Becker
D000328:Adult; D001741:Black or African American; D000368:Aged; D001241:Aspirin / Q000494:pharmacology; D003324:Coronary Artery Disease / Q000235:genetics; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010974:Platelet Aggregation / Q000187:drug effects / Q000235:genetics*; D010975:Platelet Aggregation Inhibitors / Q000494:pharmacology; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011956:Receptors, Cell Surface / Q000235:genetics*; D044465:White People
C502809:PEAR1 protein, human; D010975:Platelet Aggregation Inhibitors; D011956:Receptors, Cell Surface; D001241:Aspirin