Intracellular distribution of phototropin 1 protein in the short-day plant Ipomoea nil.
Phototropin 1 (phot1) is a blue-light Ser/Thr receptor kinase that contains two LOV domains. It is a plasma membrane-associated protein that mediates phototropism, blue-light induced chloroplast movement, and stomatal opening. The aim of the present work was to analyze the intracellular localization of phot1 protein in Ipomoea nil seedlings. In cotyledon and hypocotyl cells of etiolated seedlings, phot1 was specifically localized in the plasma membrane regions, whereas in light-treated seedlings, it was homogeneously distributed throughout the whole cytoplasm, excluding cell nuclei and vacuoles. Phot1 was also localized in cotyledon epidermal and guard cells. Such a localization pattern suggests a light-dependent intracellular distribution of phot1 in Ipomoea nil. On the basis of the spatial distribution, the possible role of phot1 is also discussed.
Department of Plant Physiology and Molecular Biology of Plants, Institute of General and Molecular Biology, Nicolaus Copernicus University, Toruń, Poland. agazet@biol.uni.torun.pl
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Kopcewicz
D018548:Cotyledon / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D003429:Cross Reactions; D056931:Cryptochromes; D005420:Flavoproteins / Q000378:metabolism* / Q000648:ultrastructure; D005455:Fluorescent Antibody Technique; D018546:Hypocotyl / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D007106:Immune Sera; D042541:Intracellular Space / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D049915:Ipomoea nil / Q000378:metabolism* / Q000528:radiation effects / Q000648:ultrastructure; D008027:Light; D021381:Protein Transport / Q000528:radiation effects; D036226:Seedlings / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects / Q000648:ultrastructure; D013997:Time Factors
D056931:Cryptochromes; D005420:Flavoproteins; D007106:Immune Sera