Different forms of MARCKS protein are involved in memory formation in the learning process of imprinting.
There is strong evidence that a restricted part of the chick forebrain, the IMM (formerly IMHV), stores information acquired through the learning process of visual imprinting. Twenty-four hours after imprinting training, a learning-specific increase in amount of myristoylated, alanine-rich C-kinase substrate (MARCKS) protein is known to occur in the homogenate fraction of IMM. We investigated the two components of this fraction, membrane-bound and cytoplasmic-phosphorylated MARCKS. In IMM, amount of membrane-bound MARCKS, but not of cytoplasmic-phosphorylated MARCKS, increased as chicks learned. No changes were observed for either form of MARCKS in PPN, a control forebrain region. The results indicate that there is a learning-specific increase in membrane-bound, non-phosphorylated MARCKS 24 h after training. This increase might contribute to stabilization of synaptic morphology.
Solomonia
Revaz O
RO
Chavachavadze Tbilisi University and Institute of Physiology, 14 L. Gotua Str., Tbilisi, 0160, Republic of Georgia.
Apkhazava
David
D
Nozadze
Maia
M
Jackson
Antony P
AP
McCabe
Brian J
BJ
Horn
Gabriel
G
D000818:Animals; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism; D002645:Chickens; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D007173:Imprinting, Psychological / Q000502:physiology*; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D007858:Learning / Q000502:physiology*; D008565:Membrane Proteins / Q000378:metabolism*; D008568:Memory / Q000502:physiology*; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D009474:Neurons / Q000378:metabolism*; D010766:Phosphorylation; D016548:Prosencephalon / Q000033:anatomy & histology / Q000254:growth & development / Q000378:metabolism*; D020033:Protein Isoforms / Q000378:metabolism; D011493:Protein Kinase C / Q000378:metabolism; D013347:Subcellular Fractions; D013569:Synapses / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology; D013997:Time Factors
D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D020033:Protein Isoforms; D000076250:Myristoylated Alanine-Rich C Kinase Substrate; D011493:Protein Kinase C