Evaluation of gelatin/resorcinol/aldehyde as a hemostatic agent and tissue adhesive: an experimental study in rat.
Use of gelatin/resorcinol/formaldehyde glue as a tissue adhesive and hemostatic agent was evaluated experimentally after modification of the aldehyde component. Gelatin/ resorcinol/aldehyde (GRA) glue was prepared by mixing gelatin and resorcinol and cross-linking with a blend of formaldehyde/glutaraldehyde. After wedge resection of the liver of 28 albino rats, bleeding was controlled, and the cut surfaces were joined with GRA. Liver biopsy was done at 7, 14, 21, and 28-day intervals. Hemostasis was excellent in 71.4%, satisfactory in 25.0%, and poor in 3.6%. Mean time for tissue adhesion was 2.6 minutes. No necrosis or bile leakage was seen. Visibility of site of repair decreased from 50.0% on day 7 to 14.3% on day 28. Residual glue was seen microscopically on day 28 in 43% cases. GRA is safe and good hemostatic, tissue adhesive, and sealant in rat liver.
Transplant and General Surgery, Renal and Transplant Unit, Nottingham University Hospitals, Nottingham, United Kingdom. rpss27@yahoo.co.uk
Rajinder P
RP
Singh
Veena
V
Maheshwari
Akshay K
AK
Verma
D000818:Animals; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D005557:Formaldehyde / Q000008:administration & dosage; D005780:Gelatin / Q000008:administration & dosage* / Q000009:adverse effects; D005976:Glutaral / Q000008:administration & dosage; D006488:Hemostasis, Surgical / Q000009:adverse effects / Q000379:methods*; D006498:Hepatectomy; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D019106:Postoperative Hemorrhage / Q000517:prevention & control; D051381:Rats; D012118:Resorcinols / Q000008:administration & dosage* / Q000009:adverse effects; D014014:Tissue Adhesives / Q000008:administration & dosage*; D016896:Treatment Outcome
D012118:Resorcinols; D014014:Tissue Adhesives; D005557:Formaldehyde; D005780:Gelatin; D005976:Glutaral; C031389:resorcinol