Influence of temperature on 31P NMR chemical shifts of phospholipids and their metabolites I. In chloroform-methanol-water.
Spectral overlap of (31)P NMR resonances and the lack of reproducibility in chemical shifts corresponding to phospholipids in organic solvents challenge the accuracy of band assignments and quantification. To alleviate these problems, the use of temperature coefficients is proposed. Changes in temperature enable the resolution of overlapped resonances and provide a facile approach for the computation of temperature coefficients. The coefficients were evaluated for various glycero- and sphingo-phospholipids. Their values suggest that differences in H-bonding between the phosphate and the head groups are responsible for the changes of chemical shift with temperature. Among parent phospholipids, and in addition to sphingomyelin, the smallest temperature coefficient values (closest to zero) were observed for phosphatidylcholine, phosphatidylglycerol, dihydrosphingomyelin, and cardiolipin. The highest values were exhibited by phospholipids with protonated head groups, such as phosphatidylserine and phosphatidylethanolamine. The lowest and, in fact, negative values were measured for phospholipids with an exposed phosphate group: phosphatidic acid, ceramide-1-phosphate, and dihydroceramide-1-phosphate. Diacyl, alkyl-acyl, and alkenyl-acyl phospholipids with the same head group exhibited comparable coefficients but differed slightly in chemical shifts. Compared to their parent glycerophospholipids, all lyso analogs had greater temperature coefficients, possibly due to the presence of an extra OH capable of forming a H-bond with the phosphate group.
Department of Chemistry, University of Louisville, Louisville, KY 40292, USA.
Rosendo
R
Estrada
Neal
N
Stolowich
M Cecilia
MC
Yappert
D000818:Animals; D001921:Brain / Q000378:metabolism; D002725:Chloroform / Q000737:chemistry*; D045424:Complex Mixtures / Q000378:metabolism; D004987:Ethers / Q000737:chemistry; D006859:Hydrogen / Q000737:chemistry; D006868:Hydrolysis; D008246:Lysophospholipids / Q000737:chemistry / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D000432:Methanol / Q000737:chemistry*; D010739:Phospholipase D / Q000378:metabolism; D054467:Phospholipases A2 / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*; D010759:Phosphorus Isotopes; D012756:Sheep / Q000033:anatomy & histology; D012997:Solvents / Q000737:chemistry; D013696:Temperature; D014867:Water / Q000737:chemistry*
D045424:Complex Mixtures; D004987:Ethers; D008246:Lysophospholipids; D010743:Phospholipids; D010759:Phosphorus Isotopes; D012997:Solvents; D014867:Water; D002725:Chloroform; D006859:Hydrogen; D054467:Phospholipases A2; D010739:Phospholipase D; D000432:Methanol