Influence of sickle cell disease and treatment with hydroxyurea on sperm parameters and fertility of human males.
BACKGROUND
Recent progress in the treatment of sickle cell disease, in particular the use of hydroxyurea, has considerably modified the prognosis of this disease. Many more patients now reach reproductive age. The objective of this study was to assess the potential impact of hydroxyurea on the semen of patients.


METHODS
In this retrospective multicenter study, we evaluated the sperm parameters and fertility of 44 patients and analyzed the potential impact of hydroxyurea.


RESULTS
We report data from the largest series so far of semen analyses in patients with sickle cell disease: 108 samples were analyzed, of which 76 were collected before treatment. We found that at least one sperm parameter was abnormal in 91% of the patients before treatment, in agreement with published literature. All sperm parameters seemed to be affected in semen samples collected during hydroxyurea treatment, and this impairment occurred in less than 6 months, later reaching a plateau. Furthermore, after hydroxyurea cessation, while global results in 30 patients were not statistically different before and after hydroxyurea treatment, in four individuals follow-up sperm parameters did not seem to recover quickly and the total number of spermatozoa per ejaculate fell below the normal range in about half the cases.


CONCLUSIONS
The observed alterations of semen parameters due to sickle cell disease seem to be exacerbated by hydroxyurea treatment. Until prospective studies reveal reassuring findings, we suggest that a pre-treatment sperm analysis be performed and sperm cryopreservation be offered to patients before hydroxyurea treatment.
Berthaut
Isabelle
I
Service d'Histologie, Biologie de, la Reproduction et Cytogénétique, CECOS Hôpital Tenon 4, rue de la, Chine 75020 Paris, France. jacqueline.mandelbaum@tnn.aphp.fr.
Guignedoux
Geoffroy
G
Kirsch-Noir
Frederique
F
de Larouziere
Vanina
V
Ravel
Celia
C
Bachir
Dora
D
Galactéros
Frédéric
F
Ancel
Pierre-Yves
PY
Kunstmann
Jean-Marie
JM
Levy
Laurence
L
Jouannet
Pierre
P
Girot
Robert
R
Mandelbaum
Jacqueline
J
D000293:Adolescent; D000328:Adult; D000755:Anemia, Sickle Cell / Q000150:complications* / Q000188:drug therapy*; D005298:Fertility; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D006918:Hydroxyurea / Q000627:therapeutic use*; D007248:Infertility, Male / Q000150:complications* / Q000209:etiology; D008297:Male; D008875:Middle Aged; D011379:Prognosis; D012189:Retrospective Studies; D012661:Semen / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects*
D006918:Hydroxyurea