Polymerized structure and improved physical and biochemical material properties of carboxymethyl dextran-conjugated collagen.
Pepsin-solubilized collagen (PSC) was conjugated with carboxymethyl dextran (CMD) using cyanogen bromide to obtain a PSC-CMD film having improved physical properties, physiological properties, and cell affinity. The conjugation was confirmed by the loss of the alpha- and beta-subunit chains and the polymerized band on SDS-PAGE, and by a decrease in the isoelectric point to 3.2. PSC-CMD had a large polymerized structure with the 6 PSC and 228 CMD molecules. PSC-CMD was readily soluble in water, reconstructed a matrix with a less-ordered structure and a characteristic morphological shape, and lost platelet aggregation-inducing ability. The PSC-CMD film, cross-linked by ultraviolet irradiation, exhibited reduced solubility, moderate water vapor permeability, and increased flexibility. PSC-CMD coatings exhibited good cell attachment and growth for fibroblasts and vein endothrical cells.
Department of Applied Biological Science, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
Harumi
H
Okada
Toshihiro
T
Maeda
Makoto
M
Hattori
Tadashi
T
Yoshida
Keiji
K
Yoshimura
Koji
K
Takahashi
D000590:Amino Acid Chloromethyl Ketones / Q000737:chemistry; D000818:Animals; D001669:Biochemical Phenomena; D001671:Biochemistry; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology* / Q000648:ultrastructure; D003911:Dextrans / Q000737:chemistry* / Q000648:ultrastructure; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects; D005347:Fibroblasts; D006801:Humans; D008855:Microscopy, Electron, Scanning; D010434:Pepsin A / Q000378:metabolism; D010974:Platelet Aggregation / Q000187:drug effects; D012995:Solubility; D013552:Swine; D013696:Temperature
D000590:Amino Acid Chloromethyl Ketones; D003911:Dextrans; D003094:Collagen; C014392:carboxymethyl dextran; D010434:Pepsin A