[Expression of drug resistance-associated proteins in brain of patients with refractory epilepsy].
OBJECTIVE
To study the expression of P-glycoprotein, multi-drug resistance associated protein and major vault protein in pathologic brain specimens, and to investigate their roles in the pathogenesis of refractory epilepsy.


METHODS
Immunohistochemical study was performed in pathology specimens from 18 cases of refractory epilepsy (including 5 cases of focal cortical dysplasia, 3 cases of tuberous sclerosis, 5 cases of ganglioglioma and 5 cases of dysembryoplastic neuroepithelial tumor).


RESULTS
Both the P-glycoprotein and major vault protein were localized in microvascular endothelium of the lesions. Major vault protein was also seen in balloon cells and some neuronal cells. On the other hand, multi-drug resistance associated protein was mainly localized in the neuronal component of the lesions. In general, the expression of P-glycoprotein and major vault protein in tumoral tissue was higher than that in non-tumoral tissue. The expression of multi-drug resistance associated protein and major vault protein was also different in the neoplastic glial cells of ganglioglioma and dysembryoplastic neuroepithelial tumor.


CONCLUSIONS
P-glycoprotein, multi-drug resistance associated protein and major vault protein contribute to the pathogenesis of refractory epilepsy. They may however have different roles, with different cellular localization.
Department of Pathology, Xuanwu Hospital, Capital University of Medical Sciences, Beijing 100053, China.
Wei
W
Wang
Yue-Shan
YS
Piao
Lei
L
Liu
Li
L
Chen
Li-Feng
LF
Wei
Hong
H
Yang
De-Hong
DH
Lu
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000032:analysis*; D000293:Adolescent; D000328:Adult; D001921:Brain / Q000378:metabolism; D001932:Brain Neoplasms / Q000737:chemistry; D002648:Child; D018432:Drug Resistance, Multiple; D004827:Epilepsy / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism*; D005260:Female; D018303:Ganglioglioma / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D054220:Malformations of Cortical Development / Q000235:genetics / Q000378:metabolism*; D027425:ATP-Binding Cassette, Sub-Family C Proteins / Q000235:genetics / Q000378:metabolism* / Q000494:pharmacology; D014402:Tuberous Sclerosis / Q000378:metabolism; D055815:Young Adult
D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D027425:ATP-Binding Cassette, Sub-Family C Proteins