Development and characterisation of novel cross-linked bio-elastomeric materials.
Recombinantly-engineered elastin-like polypeptides (ELPs) possess many of the favourable attributes of the native elastin protein, making them an attractive option for designing biomaterials for tissue-engineering applications. The focus of this study was to synthesize and characterise the bulk material properties of two ELP sequences, ELP2 and ELP4, cross-linked with lysine diisocyanate (LDI). The two distinct ELPs consist of repeating hydrophobic and hydrophilic cross-linking domains in a block co-polymer structure, however, differ by the number of respective domains. Depending on the conditions sets for the cross-linking reactions, two different ELP-based materials were synthesized: a gel-like relatively non-porous material and a porous foam-like material, from both ELP sequences. The physical material properties were characterised by scanning electron microscopy, compression testing, differential calorimetry analysis and swelling analysis. The bulk material properties were found to vary depending on the ELP sequence investigated. ELP gels were also found to have a more dense solidified morphology, lower compressive moduli, higher melting temperature and greater swelling capacity than the porous ELP foams. These novel cross-linked bio-elastomeric materials show promising properties for soft tissue replacement, particularly in load-bearing applications.
Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada M5S 3E5.
Elizabeth M
EM
Srokowski
Kimberly A
KA
Woodhouse
D000595:Amino Acid Sequence; D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry; D002152:Calorimetry, Differential Scanning; D003432:Cross-Linking Reagents / Q000737:chemistry*; D004548:Elasticity; D004549:Elastin / Q000737:chemistry*; D005782:Gels / Q000138:chemical synthesis* / Q000737:chemistry; D017953:Isocyanates / Q000737:chemistry*; D008239:Lysine / Q000031:analogs & derivatives* / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010455:Peptides / Q000138:chemical synthesis* / Q000737:chemistry; D013499:Surface Properties; D044366:Transition Temperature
D003432:Cross-Linking Reagents; D005782:Gels; D017953:Isocyanates; D010455:Peptides; C511489:lysine diisocyanate; D004549:Elastin; D008239:Lysine