Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications.
Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting from accumulated biomolecular mass. As an example, 50 spots for each of four proteins consisting of BSA, human serum albumin, rabbit IgG, and protein G were dynamically monitored as they captured corresponding antibodies. Dynamic measurements were made at 26 pg/mm(2) SD per spot and with a detectable concentration of 19 ng/ml. The presented method is particularly relevant for protein microarray analysis because it is label-free, simple, sensitive, and easily scales to high-throughput.
Ozkumur
Emre
E
Department of Electrical and Computer Engineering, Boston University, Boston, MA 02215, USA.
Needham
James W
JW
Bergstein
David A
DA
Gonzalez
Rodrigo
R
Cabodi
Mario
M
Gershoni
Jonathan M
JM
Goldberg
Bennett B
BB
Unlü
M Selim
MS
D000818:Animals; D000937:Antigen-Antibody Reactions; D015374:Biosensing Techniques; D002417:Cattle; D006801:Humans; D007201:Indicator Dilution Techniques; D007700:Kinetics; D040081:Protein Array Analysis / Q000379:methods*; D011817:Rabbits; D013194:Staining and Labeling / Q000379:methods*