Immunoadjuvant activity of crude lectin extracted from Momordica charantia seed.
The aim of this study is to investigate the immunoadjuvant activity of the crude Momordica charantia lectin (crMCL) extracted from seed using beta-galactosidase (beta-gal) as the model antigen. BALB/c mice were injected intramuscularly with beta-gal alone or beta-gal + crMCL for up to four immunizations at two-week intervals. After administration of 2 doses, the IgG-specific titer to beta-gal was significantly higher in mice in the beta-gal + crMCL group than in that from the animals from the beta-gal alone group, while it was about the same in both groups after 1 dose. Our data suggest that crMCL may help raise antibodies under the prime and boost administration regimen and could be a potent vaccine adjuvant.
Department of Veterinary Microbiology, University of Miyazaki, Miyazaki, Japan.
Li
L
Huang
Ai
A
Ikejiri
Yuya
Y
Shimizu
Takumi
T
Adachi
Yoshitaka
Y
Goto
Jun
J
Toyama
Hidenori
H
Tanaka
Ryo
R
Akashi
Kazuyuki
K
Uchida
Hironori
H
Miyata
Takeshi
T
Haga
D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D037102:Lectins / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D029775:Momordica / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D001616:beta-Galactosidase / Q000276:immunology*
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D037102:Lectins; D001616:beta-Galactosidase