Validation of a low cost computer-based method for quantification of immunohistochemistry-stained sections.
The aim of the present study was to determine if the Alpha DigiDoc RT system would be an effective method of quantifying immunohistochemical staining as compared with a manual counting method, which is considered the gold standard. Two readers were used to count 31 samples by both methods. The results obtained using the Bland-Altman for concordance deemed no statistical difference between the 2 methods. Thus, the Alpha DigiDoc RT system is an effective, low cost method to quantify immunohistochemical data.
Montgomery
Jill D
JD
Department of Infectious Diseases and Microbiology, Bloomberg School of Public Health, The Johns Hopkins University, Baltimore, MD, USA.
Hensler
Heather R
HR
Jacobson
Lisa P
LP
Jenkins
Frank J
FJ
D000951:Antigens, Neoplasm / Q000032:analysis / Q000276:immunology; D003201:Computers / Q000191:economics; D006801:Humans; D007150:Immunohistochemistry / Q000191:economics / Q000295:instrumentation / Q000379:methods*; D008297:Male; D011471:Prostatic Neoplasms / Q000737:chemistry / Q000473:pathology; D013194:Staining and Labeling / Q000191:economics / Q000295:instrumentation / Q000379:methods*
D000951:Antigens, Neoplasm