[Tissue culture and plant regeneration of Ixeridium sonchifolium].
OBJECTIVE
To study the callus induction and plant regeneration of Ixeridium sonchifolium.


METHODS
By using the orthogonal experiment design, the medium for callus induction and plant regeneration was optimized.


RESULTS
The optimal medium for callus induction was MS + 2, 4-D 1.5 mg x L(-1) +6-BA 1.5 mg x L(-1) +NAA 1.0 mg x L(-1) + IBA 1.5 mg x L(-1) + KT1.5 mg x L(-1), the optimal medium for inducing adventitious bud was MS +2, 4-D 0.2 mg x L(-1) +6-BA 0.5 mg x L(-1) + NAA 0.5 mg x L(-1) + IBA 0.5 mg x L(-1) + KT 0.5 mg x L(-1). Plantlets were rooted on 1/4MS medium supplemented with different concentrations of IBA, and high rooting and survival was achieved when the IBA concentration was 0.1 mg L(-1).


CONCLUSIONS
An efficient system for plant regeneration of I. sonchifolium was established.
College of Chinese Medicinal Material, Jilin Agricultural University, Changchun 130118, China.
Jing
J
Tao
Shi-Hai
SH
Yang
Meng-Ping
MP
Zhang
Yi-Xin
YX
Tian
D019659:Asteraceae / Q000254:growth & development* / Q000502:physiology; D010946:Plants, Medicinal / Q000254:growth & development* / Q000502:physiology; D012038:Regeneration / Q000502:physiology*; D046509:Tissue Culture Techniques / Q000379:methods*