Change of intestinal mucosa barrier function in the progress of non-alcoholic steatohepatitis in rats.
OBJECTIVE
To explore the change of intestinal mucosa barrier function in the progress of non-alcoholic steatohepatitis (NASH) in rats.


METHODS
Thirty-two Sprague-Dawley (SD) rats were randomly divided into control group and model group. Rats in the control group were given normal diet, and rats in the model group were given fat-rich diet. Eight rats in each group were killed at end of the 8th and 12th wk, respectively. The levels of endotoxin, D-xylose, TG, TC, ALT and AST, intestinal tissue SOD and MDA as well as intestinal mucus secretory IgA (sIgA) were measured. The pathology of liver was observed by HE staining.


RESULTS
At end of the 8th wk, there was no marked difference in the levels of endotoxin, D-xylose and sIgA between the two groups. At end of the 12th wk, rats in the model group developed steatohepatitis and had a higher serum level of endotoxin (P = 0.01) and D-xylose (P = 0.00) and a lower serum level of sIgA (P = 0.007).


CONCLUSIONS
Intestinal mucosa barrier malfunction may exist in NASH rats and may be an important promoter of NASH in rats.
Li
Sheng
S
Department of Gastroenterology, Yijishan Hospital, Wuhu 241001, Anhui Province, China.
Wu
Wan-Chun
WC
He
Chi-Yi
CY
Han
Zhen
Z
Jin
Dao-You
DY
Wang
Lin
L
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001426:Bacterial Proteins / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D004195:Disease Models, Animal; D018450:Disease Progression; D004731:Endotoxins / Q000097:blood; D005234:Fatty Liver / Q000378:metabolism* / Q000473:pathology; D005260:Female; D007413:Intestinal Mucosa / Q000201:enzymology / Q000378:metabolism* / Q000382:microbiology / Q000473:pathology; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010539:Permeability; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014280:Triglycerides / Q000097:blood; D014994:Xylose / Q000097:blood
D001426:Bacterial Proteins; D004731:Endotoxins; D014280:Triglycerides; C070683:surface layer protein A, Bacteria; D008315:Malondialdehyde; D002784:Cholesterol; D014994:Xylose; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase