Heart-cutting two-dimensional liquid chromatography methods for quantification of 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole in Class III caramel colours.
Heart-cutting two-dimensional liquid chromatography/UV detection methods were developed to determine 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole (noted as THI) in Class III caramel colours. In the first dimension, the caramel sample diluted in water was fractionated either by reversed-phase chromatography on C18 silica packing or by ion-exchange chromatography using strong cationic exchanger (SCX) stationary phase. In each case, THI elution domain was recovered in a loop and transferred to a second column packed with porous graphitic carbon (PGC) for the selective separation of THI. From the accuracy profiles with acceptance limit fixed at 20% and beta-expectation tolerance interval fixed at 90%, the two methods, C18-PGC and SCX-PGC, were validated for THI/caramel ratios included between 5-50 and 10-50 ppm, respectively. Finally, the two methods were compared for the analysis of numerous caramel samples.
Laboratoire des Sciences Analytiques, UMR CNRS 5180, Université Claude Bernard Lyon 1, Université de Lyon, Bâtiment CPE, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France. cedric.moretton@nigay.com
Cédric
C
Moretton
Gérard
G
Crétier
Henri
H
Nigay
Jean-Louis
JL
Rocca
D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D005505:Food Coloring Agents / Q000737:chemistry*; D007093:Imidazoles / Q000032:analysis*; D015203:Reproducibility of Results
D005505:Food Coloring Agents; D007093:Imidazoles; C049929:2-acetyl-4(5)-tetrahydroxybutylimidazole