The intron-containing genome of the lytic Pseudomonas phage LUZ24 resembles the temperate phage PaP3.
The virulent Pseudomonas aeruginosa bacteriophage LUZ24 (45,625 bp) was isolated from hospital sewage. It belongs to the family of the Podoviridae, and carries a bidirectionally transcribed dsDNA genome delineated by two direct terminal repeats of 184 bp. In vitro transcriptional analysis identified seven sigma(70) promoters, revealing a bias towards stronger promoter strength in the late genomic region. Reverse transcription demonstrated in vivo splicing of a 668 bp Group I intron embedded inside the DNA polymerase gene. Using mass spectrometry, nine structural proteins were identified as part of the phage particle. The lytic characteristics of LUZ24 are evaluated against its genomic content, which displays an overall 71% sequence similarity to the temperate phage PaP3.
Ceyssens
Pieter-Jan
PJ
Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Hertveldt
Kirsten
K
Ackermann
Hans-W
HW
Noben
Jean-Paul
JP
Demeke
Mekonnen
M
Volckaert
Guido
G
Lavigne
Rob
R
D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D007438:Introns / Q000235:genetics*; D016366:Open Reading Frames; D017105:Pseudomonas Phages / Q000145:classification / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*
D004279:DNA, Viral