The Sp1/Egr1-tandem repeat polymorphism in the 5-lipoxygenase gene promoter is not associated with late onset Alzheimer disease.
Arachidonate 5-lipoxygenase plays an important role in the synthesis of leukotrienes. Leukotrienes are inflammatory mediators, and inflammation has been implicated in the pathogenesis of Alzheimer disease. A polymorphism in the ALOX5 promoter consisting on 3 to 6 tandem-repeats of a Sp1/Egr1 binding motif (GGGCGG)n, has been related with the amount of gene expression. To verify the association between this polymorphism and the risk for late-onset Alzheimer disease we genotyped a total of 291 patients (mean age 74+/-7 y) and 300 controls (mean age 73+/-8 y). We found alleles of 3 to 6 repeats, and allele and genotype frequencies did not differ between patients and controls. These frequencies did not differ between patients according to the APOE genotype (epsilon 34 + epsilon 44 vs. epsilon 23 + epsilon 33). Together, our results indicate that the Sp1/Egr1-repeat polymorphism in the ALOX5 promoter is not a genetic marker for the risk of developing late-onset Alzheimer disease.
Genética Molecular, Hospital Universitario Central de Asturias 33006, Oviedo, Spain.
Victoria
V
Alvarez
Pelayo
P
González
Ana I
AI
Corao
Manuel
M
Menéndez
Carlos H
CH
Lahoz
Carmen
C
Martínez
Maite
M
Calatayud
Blanca
B
Morales
Eliecer
E
Coto
D017668:Age of Onset; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000201:enzymology / Q000235:genetics*; D001057:Apolipoproteins E / Q000235:genetics; D001094:Arachidonate 5-Lipoxygenase / Q000235:genetics* / Q000378:metabolism; D016022:Case-Control Studies; D051766:Early Growth Response Protein 1 / Q000235:genetics; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic / Q000235:genetics; D016329:Sp1 Transcription Factor / Q000378:metabolism; D013030:Spain; D020080:Tandem Repeat Sequences / Q000235:genetics*
D001057:Apolipoproteins E; C497325:EGR1 protein, human; D051766:Early Growth Response Protein 1; D016329:Sp1 Transcription Factor; D001094:Arachidonate 5-Lipoxygenase