[Application of pulse-field gel electrophoresis analysis in source-tracking of food-borne disease caused by Vibrio parahaemolyticus].
OBJECTIVE
To apply pulse-field gel electrophoresis analysis(PFGE) in analysing a case of food poisoning caused by Vibrio parahaemolyticus.


METHODS
PFGE using restriction enzyme Not I was employed in molecular subtyping of thirty strains of V. parahaemolyticus isolated from a case of food poisoning in Guangzhou city and PFGE patterns were analyzed by using BioNumerics Version 4.0 software to perform cluster analysis. Pattern profiles were compared by using the Dice coefficient and unweighted pair group method with arithmetic averages (UPGMA).


RESULTS
Thirty strains were of the same type of pulsotype.


CONCLUSIONS
Molecular subtyping by PFGE might disclose the epidemiological relationships of the strains from humans, food and the environment, giving a strong molecular epidemiological evidence and a support for the source-tracking of outbreak events.
Deng
Zhi-Ai
ZA
Guangzhou Center for Disease Control and Prevention, Guangzhou 510080, China.
Li
Xiao-Quan
XQ
Hu
Yu-Shan
YS
Liu
Jun-Hua
JH
Zhang
Xin-Qiang
XQ
Lin
Yun-Wan
YW
Mo
Zi-Yao
ZY
D015373:Bacterial Typing Techniques / Q000379:methods*; D002681:China; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005517:Foodborne Diseases / Q000382:microbiology*; D006801:Humans; D014736:Vibrio parahaemolyticus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification