Unique cytokine production profile following stimulation with DNA in macrophages from NZB/W F1 mice.
Nucleosome is the major autoantigen in systemic lupus erythematosus (SLE). Professional antigen-presenting cells (APCs), such as macrophages (M Phis) and dendritic cells (DCs), play the central roles in the acquisition of Ag-specific immune responses and activation of such APCs is required for the efficient Ag-presentation. Therefore, adjuvant activity of DNA in nucleosomes would cause the prominent effects on the production of anti-nucleosome antibodies. In this study, we report that elicited peritoneal M Phis from New Zealand Black/White F1 (NZB/W) mice showed a unique cytokine production profile following stimulation with DNA. M Phis from 5-week old NZB/W mice produced a higher amount of IL-6 and about a half amount of TNF-alpha after stimulation with DNA complexed with cationic liposomes compared with those from control ICR mice. These results suggest that M Phis of NZB/W mice have altered responsiveness to DNA and this might elevate the antigenicity of nucleosomes to induce the production of anti-nucleosome antibodies.
Department of Biopharmaceutics and Drug Metabolism, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto, Japan.
Yoshiyuki
Y
Ogawa
Takaharu
T
Yoshinaga
Makiya
M
Nishikawa
Yoshinobu
Y
Takakura
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000096:biosynthesis*; D004247:DNA / Q000494:pharmacology*; D005822:Genetic Vectors; D008081:Liposomes; D008264:Macrophages / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D008814:Mice, Inbred NZB; D009707:Nucleosomes
D016207:Cytokines; D008081:Liposomes; D009707:Nucleosomes; D004247:DNA