Mesoporous silica templated biolabels with releasable fluorophores for immunoassays.
A novel class of fluorescent immuno-biolabeling systems with extremely high F/P ratio (approximately 1000-6000) were prepared by combining the template method and layer-by-layer (LbL) technique. Labels were constructed by loading organic dye fluorescein diacetate (FDA) molecules onto hollow periodic mesoporous organosilica (H-PMO) particles followed by ployelectrolyte encapsulation and antibody attachment. The labeling systems were stimuli-responsive to the addition of concentrated NaOH with the loaded dye molecules being released and detected in a well-controlled manner. When applied in sandwich immunoassays, results indicated that the biolabels were immuno-active and generated an optimal signal that was approximately 50 times higher than the conventional dye labelled antibody system.
ARC Centre of Excellence for Functional Nanomaterials, The University of Queensland, St. Lucia, 4072, Australia.
Wenyi
W
Cai
Ian R
IR
Gentle
Gao Qing
GQ
Lu
Jun-Jie
JJ
Zhu
Aimin
A
Yu
D000906:Antibodies / Q000737:chemistry / Q000276:immunology; D004573:Electrolytes; D005452:Fluoresceins / Q000032:analysis* / Q000737:chemistry; D007118:Immunoassay / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D015394:Molecular Structure; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide; D013499:Surface Properties; D013696:Temperature
D000906:Antibodies; D004573:Electrolytes; D005452:Fluoresceins; D012822:Silicon Dioxide; C018506:diacetylfluorescein