Simultaneous determination of diastereomers (+)-licarin A and isolicarin A from Myristica fragrans in rat plasma by HPLC and its application to their pharmacokinetics.
A rapid and simple high-performance liquid chromatographic (HPLC) method was developed and validated to simultaneously analyze the diastereomers of (+)-licarin A and isolicarin A in rat plasma after intravenous administration. The analytes were extracted from the plasma by solid-phase extraction (SPE). Diastereomeric separation and determination were successfully achieved using a Diamonsil ODS C (18) reversed-phase column (250 mm x 4.6 mm i. d., 5 microm) with an RP18 guard column (8 mm x 4.6 mm i. d., 5 microm) and a mobile phase of MeOH-H (2)O (4 : 1, v/v). UV detection was at 270 nm. The linear ranges of the standard curves were 0.25 - 150.00 microg/mL for (+)-licarin A and 0.10 - 75.00 microg/mL for isolicarin A. The lower limits of detection and quantification were 0.05 and 0.25 microg/mL for (+)-licarin A, and 0.05 and 0.10 microg/mL for isolicarin A, respectively. This assay method was successfully applied to study the pharmacokinetics of diastereomers (+)-licarin A and isolicarin A in rat plasma.
Li
Fei
F
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing, People's Republic of China.
Yang
Xiu-Wei
XW
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D017705:Lignans / Q000097:blood* / Q000493:pharmacokinetics; D008968:Molecular Conformation; D026323:Myristica / Q000737:chemistry*; D051381:Rats; D014466:Ultraviolet Rays
D017705:Lignans; C432503:licarin A