The effects of pharmaceuticals on the regeneration of the cnidarian, Hydra attenuata.
The Hydra attenuata regeneration assay was used to identify the teratogenic potential of 10 pharmaceuticals identified in effluent from a large city wastewater treatment plant (WWTP). Three types of solvents were used to solubilise the pharmaceuticals (DMSO, acetone and ethanol), at concentrations determined to have no significant effect on measured endpoints. On the one hand, regeneration was significantly inhibited at (nominal) concentrations of 1, 5 and 1 mg/L for gemfibrozil, ibuprofen and naproxen respectively and at the higher concentration of 50 mg/L for bezafibrate and trimethoprim. On the other hand, carbamazepine and the antibiotics sulfapyridine, oxytetracycline and novobiocin significantly increased regeneration at 25, 5, 50 and 50 mg/L respectively. Relatively high IC50 values of 0.9, 3.84, 4.9 and 22.5 mg/L were calculated for gemfibrozil, ibuprofen, naproxen and bezafibrate, respectively. However when subjected to tier two toxicity assessment under EU regulatory guidance using environmentally relevant concentrations a MEC/PNEC value>1 was calculated for gemfibrozil, ibuprofen and naproxen indicating teratogenic potential and the necessity for further tier three assessment. A toxicity index (TI) was also calculated using three different techniques, with TI values>3 (indicating teratogenic potential) found for gemfibrozil, ibuprofen, naproxen and bezafibrate and >1 (indicating a weak teratogenic potential) found for carbamazepine. These results are discussed in the context of their environmental relevance and toxic potential.
Quinn
Brian
B
Fluvial Ecosystem Research, Aquatic Ecosystem Protection Research Division, Water Science and Technology, Environment Canada, 105 McGill, Montréal, Québec, Canada H2Y 2E7. quinnbrian@gmail.com
Gagné
François
F
Blaise
Christian
C
D000465:Algorithms; D018712:Analgesics, Non-Narcotic / Q000633:toxicity; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity; D001681:Biological Assay / Q000379:methods*; D002947:Cities; D064420:Drug-Related Side Effects and Adverse Reactions; D004784:Environmental Monitoring / Q000379:methods*; D006829:Hydra / Q000187:drug effects* / Q000378:metabolism; D000960:Hypolipidemic Agents / Q000633:toxicity; D007220:Industrial Waste; D004364:Pharmaceutical Preparations / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D018675:Toxicity Tests; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D018712:Analgesics, Non-Narcotic; D000900:Anti-Bacterial Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000960:Hypolipidemic Agents; D007220:Industrial Waste; D004364:Pharmaceutical Preparations; D012997:Solvents; D014874:Water Pollutants, Chemical