[Interventional effect of emodin on the expression of intergern linked kinase in tubular epithelial-myofibroblast transdifferentiation].
OBJECTIVE
To observe the change of ILK expression in interleukin-1beta(IL-1beta)-induced tubular epithelial-myofibroblast transdifferentiation, and to investigate whether emodin inhibit IL-1beta-induced tubular epithelial-myofibroblast transdifferentiation through an intergern linked kinase-dependent mechanism.


METHODS
Normal rat kidney epithelial cell line (NRK52E) was cultured and then divided into blank group, emodin control group, IL-1beta-induced group and emodin-inhibited group. When the cells were cultured for 48 h, their morphological changes were observed by an inverted phase contrast microscope. The expression of a-smooth muscle actin (a-SMA) and E-cadherin were detected using a two-color immunohistochemistry staining technique, while the expression of integrin-linked kinase (ILK) was detected using a one-color immunohistochemistry staining technique. The secretion of fibronectin (FN) was analyzed by ELISA.


RESULTS
NRK52E cells cultured with IL-1 became fibroblast-like in appearance. The expression of a-SMA was enhanced (65h5+/-1h7 vs 140h4+/-3h0, P<0h05), the expression of E-cadherin was decreased (82h5+/-1h0 vs 36h0+/-2h8, P<0h05), the expression of ILK was enhanced (36h1+/-3h1 vs 82h4+/-1h2, P<0h05), and the secretion of FN was increased (54h6+/-3h1 vs 124h8+/-3h2 mg/L, P<0h05). Emodin markedly inhibited all of those changes induced by IL-1beta.


CONCLUSIONS
The expression of ILK is up-regulated in IL-1beta-induced tubular epithelial-myofibroblast transdifferentiation. Emodin might inhibit TEMT by a down-regulation the expression of ILK.
Chen
Ting-fang
TF
Department of Nephrology, Affiliated Hospital of Luzhou Medical College, Luzhou, China. chen_yue16@yahoo.com.cn
Chen
Ming
M
Qin
Jian-hua
JH
Wang
Ming-yong
MY
D000818:Animals; D002460:Cell Line; D054338:Cell Transdifferentiation / Q000187:drug effects; D004642:Emodin / Q000494:pharmacology*; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006168:Guinea Pigs; D053583:Interleukin-1beta / Q000378:metabolism; D007684:Kidney Tubules / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D051379:Mice; D032446:Myoblasts / Q000166:cytology* / Q000201:enzymology; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D051381:Rats; D000099200:Scaffold Protein ILK
D004642:Emodin; D053583:Interleukin-1beta; D017346:Protein Serine-Threonine Kinases; D000099200:Scaffold Protein ILK