Detection of intact influenza viruses using biotinylated biantennary S-sialosides.
We report the modular synthesis of robust, biotinylated biantennary sialylglycoconjugates and their ability to differentiate between two type A influenza strains. This is the first demonstration of glycoconjugate-based discriminatory capture and detection of two strains of intact influenza virus, in the presence of the innate enzymatic activity of viral neuraminidases. We also demonstrate a "carboassay" using glycoconjugates as capture and reporter elements, which therefore, does not require antibodies. The capture of intact influenza viruses is of potential benefit for clinical diagnostics.
Chemical and Biosensors group, 805 Crosley, Department of Chemistry, University of Cincinnati, Cincinnati, Ohio 45221, USA.
Ramesh R
RR
Kale
Harshini
H
Mukundan
Dominique N
DN
Price
J Foster
JF
Harris
Daniel M
DM
Lewallen
Basil I
BI
Swanson
Jurgen G
JG
Schmidt
Suri S
SS
Iyer
D019921:Biotinylation; D006001:Glycoconjugates; D009975:Orthomyxoviridae / Q000302:isolation & purification*; D012794:Sialic Acids; D013057:Spectrum Analysis
D006001:Glycoconjugates; D012794:Sialic Acids