[Effect of divalent metal ions on DNA conformational transitions in water-ethanol solutions].
The influence of different MgCl2 and MnCl2 concentrations on DNA conformational transitions in water-ethanol solutions was studied. It was shown that the presence of magnesium ions in solution at a concentration of 5 x 10(-4) M did not influence the decrease in the size of DNA without change in its persistent length at an alcohol concentration of about 17 % v/v. In contrast, manganese ions prevent this change in DNA parameters. At sufficiently high ethanol concentrations, the compaction of DNA followed by its precipitation takes place, which is accompanied by an increase of scattering in solution. As the concentration of Mg2+ and Mn2+ in solution increases, this process is observed at lower ethanol concentrations.
N A
NA
Kas'ianenko
I Iu
IIu
Pereviazko
M I
MI
Blokova
I M
IM
Zyrianova
D002413:Cations, Divalent; D002712:Chlorides / Q000737:chemistry*; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D015636:Magnesium Chloride / Q000737:chemistry*; D017895:Manganese Compounds / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D012996:Solutions; D013056:Spectrophotometry, Ultraviolet
D002413:Cations, Divalent; D002712:Chlorides; D017895:Manganese Compounds; D012996:Solutions; D015636:Magnesium Chloride; D004247:DNA; C025340:manganese chloride