Screen for leukotoxin mutants in Aggregatibacter actinomycetemcomitans: genes of the phosphotransferase system are required for leukotoxin biosynthesis.
Aggregatibacter (formerly Actinobacillus) actinomycetemcomitans is a pathogen that causes localized aggressive periodontitis and extraoral infections including infective endocarditis. Recently, we reported that A. actinomycetemcomitans is beta-hemolytic on certain growth media due to the production of leukotoxin (LtxA). Based on this observation and our ability to generate random transposon insertions in A. actinomycetemcomitans, we developed and carried out a rapid screen for LtxA mutants. Using PCR, we mapped several of the mutations to genes that are known or predicted to be required for LtxA production, including ltxA, ltxB, ltxD, and tdeA. In addition, we identified an insertion in a gene previously not recognized to be involved in LtxA biosynthesis, ptsH. ptsH encodes the protein HPr, a phosphocarrier protein that is part of the sugar phosphotransferase system. HPr results in the phosphorylation of other proteins and ultimately in the activation of adenylate cyclase and cyclic AMP (cAMP) production. The ptsH mutant showed only partial hemolysis on blood agar and did not produce LtxA. The phenotype was complemented by supplying wild-type ptsH in trans, and real-time PCR analysis showed that the ptsH mutant produced approximately 10-fold less ltxA mRNA than the wild-type strain. The levels of cAMP in the ptsH mutant were significantly lower than in the wild-type strain, and LtxA production could be restored by adding exogenous cAMP to the culture.
Department of Oral Biology, University of Medicine and Dentistry of New Jersey, 185 S. Orange Avenue, Medical Science Building C-636, Newark, NJ 07103, USA.
Maria P
MP
Isaza
Matthew S
MS
Duncan
Jeffrey B
JB
Kaplan
Scott C
SC
Kachlany
D016976:Aggregatibacter actinomycetemcomitans / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics*; D000595:Amino Acid Sequence; D003470:Culture Media; D000242:Cyclic AMP / Q000032:analysis; D004251:DNA Transposable Elements; D005098:Exotoxins / Q000096:biosynthesis*; D020869:Gene Expression Profiling; D005816:Genetic Complementation Test; D006461:Hemolysis; D006801:Humans; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System / Q000235:genetics*; D016133:Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016415:Sequence Alignment
D003470:Culture Media; D004251:DNA Transposable Elements; D005098:Exotoxins; C018579:leukotoxin; D000242:Cyclic AMP; D010731:Phosphoenolpyruvate Sugar Phosphotransferase System