Placental secreted factors: their role in the regulation of anti-CII antibodies and amelioration of collagen induced arthritis in rats.
Pregnancy induces collagen-induced arthritis (CIA) remission in rats. Placental hormones, cytokines and growth factors can regulate immune cell activity at the feto-maternal interface as well as at the systemic level. We assessed the effect of placental culture supernatants (PS) in CIA developed in rats after the inoculation of collagen type II (CII) in complete Freund's adjuvant. After the onset of CIA, animals were injected by ip route with seven doses of PS. On the 18th day of treatment with PS, serum anti-CII antibody (total IgG, IgG(1), IgG(2a), IgG(2b), IgG asymmetric molecules) and cytokine levels were determined by ELISA. An arthritic index was used by daily measure of joint swelling and visual signs of arthritis. Our results demonstrated that the PS treatment diminished CIA symptoms, reduced TNF-alpha, INF-gamma and anti-CII antibody serum levels, increased the proportion of asymmetric IgG anti-CII antibodies and affected IgG(1)/IgG(2a) and IgG(1)/IgG(2b) ratio. Two weeks after the last PS inoculation there was a recurrence of arthritis, a rise in IgG anti-CII and, simultaneously, the percentage of asymmetric IgG anti-CII fell. We concluded that PS have an effective CIA suppressor activity partly due to the modulation of humoral immune response and may be closely related to an inhibitory effect on TNF-alpha and INF-gamma production.
Cátedra de Inmunología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires (UBA), Instituto de Estudios de la Inmunidad Humoral Dr. Ricardo Margni (CONICET-UBA), Junín 956, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina.
Micaela
M
Cortés
Andrea
A
Canellada
Silvia
S
Miranda
José
J
Dokmetjian
Teresa
T
Gentile
D000818:Animals; D000906:Antibodies / Q000276:immunology; D000917:Antibody Formation / Q000187:drug effects / Q000276:immunology*; D001169:Arthritis, Experimental / Q000097:blood / Q000276:immunology* / Q000503:physiopathology / Q000628:therapy; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D024043:Collagen Type II / Q000008:administration & dosage / Q000276:immunology*; D017077:Culture Media, Conditioned / Q000494:pharmacology; D016207:Cytokines / Q000037:antagonists & inhibitors / Q000378:metabolism; D005260:Female; D007074:Immunoglobulin G / Q000097:blood* / Q000276:immunology; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D012720:Severity of Illness Index
D000906:Antibodies; D024043:Collagen Type II; D017077:Culture Media, Conditioned; D016207:Cytokines; D007074:Immunoglobulin G