Liver histological, portal flow and plasmatic nitric oxide alterations caused by biliary obstruction and drainage in rats.
OBJECTIVE
To evaluate liver alterations caused by biliary obstruction and drainage.


METHODS
Thirty-nine male Wistar rats were randomly distributed in 4 groups: BO (n=18) bile duct ligation for 20 days, with a periodic evaluation of liver histological alterations, Doppler echography portal flow and measurements of NO and malondialdehyde (MDA); BO/DB (n=13) bile duct occlusion for 20 days followed by biliary drainage by choledochoduodenal anastomosis, 5 days follow-up, same BO group parameters evaluations; group CED (n=4) sham operation and portal flow evaluation trough 20 days; CHB (n=4) sham operation, with hepatic biopsy on 25th day and followed-up trough 25 days, by the same parameters of group BO, with exception of portal flow. Direct bilirubin (DB) and alkaline phosphatase (AP) were evaluated in the group BO, BO/DB and CHB.


RESULTS
The bile duct ligation led to an increase of DB and AP, development of liver histological alterations, reduction of portal flow and increase of plasmatic NO and of MDA levels. The bile duct clearing resulted in a reduction of DB, AP, NO, MDA histological alterations and increase of portal flow.


CONCLUSIONS
The biliary occlusion resulted in cholestasis and portal flow reduction, besides the increase of plasmatic NO and of hepatic MDA levels, and histological liver alterations, with a tendency of normalization after the bile duct clearing.
Dias
Miguel Angel
MA
Department of Surgery and Anatomy, Ribeirão Preto Faculty of Medicine, University of São Paulo, SP, Brazil.
Ceneviva
Reginaldo
R
Elias
Jorge
J
Zucoloto
Sergio
S
Baldo
Caroline Floreoto
CF
Evora
Paulo Roberto Barbosa
PR
D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D001663:Bilirubin / Q000097:blood; D015415:Biomarkers / Q000032:analysis; D001651:Cholestasis, Extrahepatic / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D004195:Disease Models, Animal; D006500:Hepatic Duct, Common / Q000473:pathology / Q000503:physiopathology* / Q000601:surgery; D015227:Lipid Peroxidation / Q000502:physiology; D008099:Liver / Q000098:blood supply / Q000503:physiopathology* / Q000601:surgery; D008102:Liver Circulation / Q000502:physiology*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009569:Nitric Oxide / Q000097:blood*; D011169:Portal Vein / Q000503:physiopathology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D015415:Biomarkers; D009569:Nitric Oxide; D008315:Malondialdehyde; D000469:Alkaline Phosphatase; D001663:Bilirubin