Characterization of Leishmania (Leishmania) amazonensis promastigotes resistant to pentamidine.
Pentamidine is a second-line agent used in the treatment of leishmaniasis and its mode of action and mechanism of resistance is not well understood. It was previously demonstrated that transfection of promastigotes and amastigotes with the ABC transporter PRP1 gene confers resistance to pentamidine. To further clarify this point, we generated Leishmania amazonensis mutants resistant to pentamidine. Our results indicated that this ABC transporter is not associated with pentamidine resistance in lines generated by drug pressure through amplification or overexpression mechanisms of PRP1 gene.
Instituto de Medicina Tropical, Departamento Moléstias infecciosas e Parasitárias, Faculdade de Medicina da Universidade de São Paulo, Av. Dr. Enéas de Carvalho Aguiar, 470, 4 degrees andar, 05403-000 São Paulo, SP, Brazil.
Adriano C
AC
Coelho
Luciana G
LG
Gentil
José Franco
JF
da Silveira
Paulo C
PC
Cotrim
D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000502:physiology; D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D015139:Blotting, Southern; D016054:DNA, Protozoan / Q000737:chemistry / Q000302:isolation & purification; D004351:Drug Resistance / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005456:Fluorescent Dyes; D007211:Indoles; D020128:Inhibitory Concentration 50; D007894:Leishmania mexicana / Q000187:drug effects* / Q000235:genetics; D008856:Microscopy, Fluorescence; D009154:Mutation; D010419:Pentamidine / Q000494:pharmacology*; D010641:Phenotype; D015800:Protozoan Proteins / Q000235:genetics / Q000502:physiology
D018528:ATP-Binding Cassette Transporters; D000981:Antiprotozoal Agents; D016054:DNA, Protozoan; D005456:Fluorescent Dyes; D007211:Indoles; D015800:Protozoan Proteins; C480121:pentamidine resistance protein 1, Leishmania major; C007293:DAPI; D010419:Pentamidine