Autonomous Hedgehog signalling is undetectable in PC-3 prostate cancer cells.
The Hedgehog signalling pathway has been implicated in the development of prostate cancer, although this area remains controversial. Some but not all studies have noted relatively high Hedgehog pathway activity in commonly used prostate cancer cell lines. We aimed to evaluate the widely used PC-3 cell line as a model to investigate Hedgehog signalling in a prostate cancer setting. Using a sensitive Hedgehog inducible luciferase reporter assay, we found no evidence of autonomous Hedgehog signalling in PC-3 cells, irrespective of passage number. In addition, manipulations that should either increase (an oxysterol) or decrease (cyclopamine) Hedgehog pathway activity had no effect on reporter activity, and cyclopamine treatment did not affect PC-3 cell viability. Therefore, our findings contradict some earlier reports and caution against the use of PC-3 cells to investigate the Hedgehog pathway in a prostate cancer setting.
BABS, School of Biotechnology and Biomolecular Sciences, Biosciences Building D26, University of New South Wales, Sydney, NSW 2052, Australia.
Frank R K
FR
McCarthy
Andrew J
AJ
Brown
D045744:Cell Line, Tumor; D017930:Genes, Reporter; D053823:Hedgehog Proteins / Q000378:metabolism*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008297:Male; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D014053:Tomatine / Q000031:analogs & derivatives / Q000494:pharmacology; D014704:Veratrum Alkaloids / Q000494:pharmacology
D053823:Hedgehog Proteins; D014704:Veratrum Alkaloids; C003677:tomatidine; D014053:Tomatine; D008156:Luciferases; C000541:cyclopamine