Rhodococcus kunmingensis sp. nov., an actinobacterium isolated from a rhizosphere soil.
A Gram-positive, aerobic, non-motile actinobacterium strain, designated YIM 45607(T), was isolated from a rhizosphere soil sample in Kunming, south-west China. Chemotaxonomically, the isolate contained chemical markers that supported its assignment to the genus Rhodococcus. On the basis of 16S rRNA gene sequence similarity analysis, strain YIM 45607(T) formed a new subline within the genus Rhodococcus, with Rhodococcus equi as its closest phylogenetic neighbour (98.2 % 16S rRNA gene sequence similarity to the type strain). However, DNA-DNA hybridization demonstrated that strain YIM 45607(T) was different from R. equi DSM 20307(T) (35.4 % relatedness). Based on polyphasic analysis, strain YIM 45607(T) could be clearly distinguished from other species of the genus Rhodococcus. The isolate therefore represents a novel species of Rhodococcus, for which the name Rhodococcus kunmingensis sp. nov. is proposed. The type strain is strain YIM 45607(T) (=KCTC 19149(T) =DSM 45001(T)).
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China.
Yong-Xia
YX
Wang
Hai-Bin
HB
Wang
Yu-Qin
YQ
Zhang
Li-Hua
LH
Xu
Cheng-Lin
CL
Jiang
Wen-Jun
WJ
Li
D015373:Bacterial Typing Techniques; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012240:Rhodococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity; D020946:Taxus / Q000382:microbiology*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S