Direct plant gene delivery with a poly(amidoamine) dendrimer.
Plant gene delivery is challenging due to the presence of plant cell walls. Conventional means such as Agrobacterium infection, biolistic particle bombardment, electroporation, or polyethylene glycol attachment are often characterized by high cost, labor extensiveness, and a significant perturbation to the growth of cells. We have succeeded in delivering GFP-encoding plasmid DNA to turfgrass cells using poly(amidoamine) dendrimers. Our new scheme utilizes the physiochemical properties as well as the nanosize of the poly(amidoamine) dendrimer for direct and noninvasive gene delivery. The GFP gene was expressed in the plant cells as observed by confocal fluorescence microscopy. The transfection efficiency may be further improved by optimizing the pH of the cell culture medium and the molar ratio of the dendrimer to DNA. The use of the current delivery system can be extended to virtually all plant species having successful regeneration systems in place.
Pasupathy
Karthikeyan
K
Laboratory of Single-Molecule Biophysics and Polymer Physics, Department of Physics and Astronomy, Clemson University, Clemson, SC, USA.
Lin
Sijie
S
Hu
Qian
Q
Luo
Hong
H
Ke
Pu Chun
PC
D004247:DNA / Q000235:genetics*; D050091:Dendrimers; D004337:Drug Carriers / Q000737:chemistry; D018014:Gene Transfer Techniques; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism*; D011073:Polyamines / Q000737:chemistry*; D011994:Recombinant Proteins / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
D050091:Dendrimers; D004337:Drug Carriers; C104700:PAMAM Starburst; D010940:Plant Proteins; D011073:Polyamines; D011994:Recombinant Proteins; D004247:DNA