Comparison between genetic portraits of osteoblasts derived from primary cultures and osteoblasts obtained from human pulpar stem cells.
Harvesting bone for autologous grafting is a daily problem encountered by craniofacial and oral surgeons. Stem cells derived from human dental pulp are able to differentiate in osteoblasts and are a potential source of autologous bone produced in vitro. However, as stem cells are characterized by self-renewing and commitment in several cellular subtypes (ie, pluripotential differentiation), some concerns may arise as regards their potential uncontrolled proliferation. To screen the behavior of osteoblasts derived from human pulpar stem cells (ODHPSCs), we used microarray techniques to identify genes that are differently regulated in ODHPSC in comparison to normal osteoblasts (NOs). Osteoblasts derived from human pulpar stem cells were obtained from human dental pulp, and cells were selected using a cytometer. The cell profile was c-kit+/CD34+/STRO-1+/CD45-. These cells were capable of differentiation of osteoblasts in vitro. By using DNA microarrays containing 19,200 genes, we identified in ODHPSC some genes whose expression was significantly up- and downregulated compared to NO. The differentially expressed genes have different functional activities: (a) cell differentiation, (b) developmental maturation, (c) cell adhesion, and (d) production of cytoskeleton elements. Thus, some molecular differences exist between NO and ODHPSC, although the previously considered histologic parameters show a normal phenotype.
Department of DMCCC, Section of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy. crc@unife.it
Francesco
F
Carinci
Gianpaolo
G
Papaccio
Gregorio
G
Laino
Annalisa
A
Palmieri
Giorgio
G
Brunelli
Riccardo
R
D'Aquino
Antonio
A
Graziano
Vladimiro
V
Lanza
Luca
L
Scapoli
Marcella
M
Martinelli
Furio
F
Pezzetti
D053687:Adult Stem Cells / Q000166:cytology*; D018952:Antigens, CD34 / Q000235:genetics; D000954:Antigens, Surface / Q000235:genetics; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D048429:Cell Size; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology*; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D017493:Leukocyte Common Antigens / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis; D010006:Osteoblasts / Q000166:cytology*; D019009:Proto-Oncogene Proteins c-kit / Q000235:genetics
D018952:Antigens, CD34; D000954:Antigens, Surface; C525591:STRO-1 antigen, human; D019009:Proto-Oncogene Proteins c-kit; D017493:Leukocyte Common Antigens; C516769:PTPRC protein, human