Evaluation of the potential of synthetic peptides of 80 kDa human sperm antigen (80 kDaHSA) for the development of contraceptive vaccine for male.
80 kDaHSA has been demonstrated to be responsible for inducing immunoinfertility. Synthetic peptides NT, 1, 2 and 4 of 80 kDaHSA are immunogenic and immunobiologically mimic the native protein. Peptides 1 and NT being highly immunogenic their potential for contraceptive vaccine development was evaluated. Active immunization of male rabbits with peptide-1 and -NT induced reversible infertility in 100% and 60% of animals, respectively and subsequently active immunization of non-human primate model, male marmosets with peptide-1 induced reversible infertility in six out of seven high antibody titer animals. The present study suggests the potential of peptide-1 of 80 kDaHSA for the development of contraceptive vaccine.
Department of Biochemistry, National Institute for Research in Reproductive Health (ICMR), J M Street, Parel, Mumbai 400012, India.
Bharati Gangaram
BG
Khobarekar
Vandana
V
Vernekar
Vijaya
V
Raghavan
Masaharu
M
Kamada
Masahiko
M
Maegawa
Atmaram Hari
AH
Bandivdekar
D000818:Animals; D000906:Antibodies / Q000097:blood; D000954:Antigens, Surface / Q000276:immunology*; D002144:Callithrix; D007248:Infertility, Male / Q000276:immunology; D008297:Male; D010455:Peptides / Q000138:chemical synthesis / Q000276:immunology*; D011817:Rabbits; D013081:Sperm Motility / Q000276:immunology; D013737:Testis / Q000473:pathology; D022642:Vaccines, Contraceptive / Q000276:immunology*
D000906:Antibodies; D000954:Antigens, Surface; D010455:Peptides; D022642:Vaccines, Contraceptive; C509458:sperm antigen 80-kDa, human