Imaging polyhedral inclusion bodies of nuclear polyhedrosis viruses with second harmonic generation microscopy.
We studied the polarization anisotropy of second harmonic generation (SHG) in polyhedral inclusion bodies (PIBs) of nuclear polyhedrosis viruses (NPV). Due to a body-centered-cubic arrangement of polyhedrin trimers, a characteristic SHG polarization property with a mixture of I23 and I3 symmetry was measured from PIBs. With this characteristic SHG anisotropy, it provides an intrinsic nonlinear signal for virus infection studies in living cells. With multimodal harmonic generation microscopy, we also demonstrated 3D imaging on PIBs of NPV in living cells. The distribution and the number of PIBs in intact infected cells can be revealed without the help of fluorescent labeling.
Liu
Tzu-Ming
TM
Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan, ROC.
Lee
Yen-Wei
YW
Chang
Chieh-Feng
CF
Yeh
Shih-Chia
SC
Wang
Chung-Hsiung
CH
Chu
Shi-Wei
SW
Sun
Chi-Kuang
CK
D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D002479:Inclusion Bodies / Q000648:ultrastructure*; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods; D017924:Nucleopolyhedroviruses / Q000648:ultrastructure*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity