Two-layered multiphoton microscopic imaging of cervical tissue.
We demonstrate the application of multiphoton microscopy (MPM) based on two-photon excited fluorescence and second harmonic generation signals in imaging two-layered structures of cervical tissue in mice. It was found that MPM is effective for visualizing two-layered cervical microstructures, monitoring the metabolic activity and the density (nuclear-to-cytoplasmic) of epithelial cells, measuring the size of epithelial cell nuclei and the thickness of the epithelial layer, and quantifying collagen in the stroma. These results suggest that, with the advent of clinical portability of typical multiphoton endoscopes, the MPM technique has the potential to be applied in vivo to the clinical diagnosis and monitoring of cervical cancer.
Key Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Normal University, Ministry of Education, Fuzhou, 350007, China.
Shuangmu
S
Zhuo
Jianxin
J
Chen
Tianshu
T
Luo
Xingshan
X
Jiang
Shusen
S
Xie
Rong
R
Chen
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002584:Cervix Uteri / Q000033:anatomy & histology* / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005420:Flavoproteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009243:NAD / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000378:metabolism
D005420:Flavoproteins; D009243:NAD; D003094:Collagen