Full structure of the carbohydrate chain of the lipopolysaccharide of Providencia rustigianii O34.
A lipopolysaccharide isolated from an opportunistic pathogen of the Enterobacteriaceae family Providencia rustigianii O34 was found to be a mixture of R-, SR-, and S-forms consisting of a lipid moiety (lipid A) that bears a core oligosaccharide, a core with one O-polysaccharide repeating unit attached, and a long-chain O-polysaccharide, respectively. The corresponding carbohydrate moieties were released from the lipopolysaccharide by mild acid hydrolysis and studied by sugar and methylation analyses along with one- and two-dimensional NMR spectroscopy and high-resolution electrospray ionization mass spectrometry. As a result, the structures of the core and the O-polysaccharide were established, including the structure of the biological repeating unit (an oligosaccharide that is preassembled and polymerized in biosynthesis of the O-polysaccharide), as well as the mode of the linkage between the O-polysaccharide and the core. Combining the structure of the carbohydrate moiety thus determined and the known structure of lipid A enabled determination of the full lipopolysaccharide structure of P. rustigianii O34.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prospect 47, 119991 Moscow, Russian Federation.
Nina A
NA
Kocharova
Anna N
AN
Kondakova
Evgeny
E
Vinogradov
Olga G
OG
Ovchinnikova
Buko
B
Lindner
Alexander S
AS
Shashkov
Antoni
A
Rozalski
Yuriy A
YA
Knirel
D002240:Carbohydrate Sequence; D008070:Lipopolysaccharides / Q000737:chemistry*; D008969:Molecular Sequence Data; D009844:Oligosaccharides / Q000737:chemistry; D011532:Providencia / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization
D008070:Lipopolysaccharides; D009844:Oligosaccharides