Renal ischemia and reperfusion injury: influence of chorpromazine on renal function and lipid peroxidation.
OBJECTIVE
To evaluate the influence of chlorpromazine (CPZ) on renal function and lipid peroxidation in a rat model of kidney ischemia/reperfusion injury.


METHODS
Forty eight Wistar rats underwent a laparotomy for hilar clamping of left kidney with a bulldog clamp for 60 minutes followed by organ reperfusion and contralateral nephrectomy. Of these, 26 received 3mg/kg of CPZ intravenously 15 minutes before renal ischemia (G-E) while the remaining 22 were used as ischemic control group (G-C). Eleven rats of G-E and 8 of G-C were followed for blood urea nitrogen and creatinine determinations before renal ischemia and at 1st, 4th and 7th postoperative days. Samplings of left renal tissue were obtained at 5 minutes (5 rats from each group) and 24 hours (9 G-C and 10 of G-E) of reperfusion for malondialdehy (MDA) content determination. Controls of renal MDA content were determined in kidneys harvested from 6 additional normal rats.


RESULTS
Acute renal failure occurred in all animals but levels of BUN and creatinine were significantly lower in G-E (p<0.001). MDA content rose strikingly at 5 minutes of reperfusion in both groups (p>0.05) and returned near to normal levels 24 hours later.


CONCLUSIONS
CPZ conferred partial protection of renal function to kidneys submitted to ischemia/reperfusion injury that seems to be not dependent on inhibition of lipid peroxidation.
Division of Urology, Department of Surgery and Anatomy, Ribeirão Preto Faculty of Medicine, University of São Paulo, SP, Brazil. stucci@convex.com.br
Silvio
S
Tucci Junior
Roberto Marins de
RM
Carvalho
Fábia Martins
FM
Celini
Adauto José
AJ
Cologna
Haylton Jorge
HJ
Suaid
Luis Fernando
LF
Tirapelli
Antonio Carlos Pereira
AC
Martins
D000818:Animals; D015415:Biomarkers / Q000097:blood; D002746:Chlorpromazine / Q000494:pharmacology*; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D018492:Dopamine Antagonists / Q000494:pharmacology*; D007511:Ischemia / Q000150:complications*; D041781:Jaundice, Obstructive / Q000188:drug therapy; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects* / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009392:Nephrectomy; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D013997:Time Factors; D014508:Urea / Q000097:blood
D015415:Biomarkers; D018492:Dopamine Antagonists; D008315:Malondialdehyde; D014508:Urea; D003404:Creatinine; D002746:Chlorpromazine