Antibody microarrays as an experimental platform for the analysis of signal transduction networks.
A significant bottleneck for the time-resolved and quantitative description of signaling networks is the limited sample capacity and sensitivity of existing methods. Recently, antibody microarrays have emerged as a promising experimental platform for the quantitative and comprehensive determination of protein abundance and protein phosphorylation. This review summarizes the development of microarray applications involving antibody-based capture of target proteins with a focus on quantitative applications. Technical aspects regarding the production of antibody microarrays, identification of suitable detection and capture antibody pairs, signal detection methods, detection limit, and data analysis are discussed in detail.
Korf
Ulrike
U
Division of Molecular Genome Analysis, B050, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120, Heidelberg, Germany. u.korf@dkfz.de
Henjes
Frauke
F
Schmidt
Christian
C
Tresch
Achim
A
Mannsperger
Heiko
H
Löbke
Christian
C
Beissbarth
Tim
T
Poustka
Annemarie
A
D000906:Antibodies / Q000737:chemistry* / Q000276:immunology / Q000378:metabolism*; D007118:Immunoassay / Q000379:methods*; D040081:Protein Array Analysis / Q000379:methods*; D025941:Protein Interaction Mapping / Q000379:methods*; D020543:Proteome / Q000276:immunology / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
D000906:Antibodies; D020543:Proteome