Molecular characterization of porcine picobirnaviruses and development of a specific reverse transcription-PCR assay.
The molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets.
Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Argentina.
Gabriela M
GM
Carruyo
Guaniri
G
Mateu
Laura C
LC
Martínez
Flor H
FH
Pujol
Silvia V
SV
Nates
Ferdinando
F
Liprandi
Juan E
JE
Ludert
D000818:Animals; D001118:Argentina; D004591:Electrophoresis, Polyacrylamide Gel; D005243:Feces / Q000821:virology; D008969:Molecular Sequence Data; D010802:Phylogeny; D018708:Picobirnavirus / Q000235:genetics / Q000302:isolation & purification*; D012327:RNA Virus Infections / Q000175:diagnosis* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis / Q000821:virology*; D014687:Venezuela
D012367:RNA, Viral