Production of calcium-stearate by lipase using hydrogenated beef tallow.
Calcium-stearate has been traditionally produced by chemical methods, producing wastes and requiring high energy because of high temperature operation. To achieve enzymatic production of calcium-stearate at unfavorable conditions, i.e., pH 10 and 60 degrees C, suitable lipase was selected and reaction conditions were optimized using calcium hydroxide and hydrogenated beef tallow as substrates. Under optimum conditions, 95% of beef tallow, in 2.5 h, was converted into calcium-stearate by using commercial lipase SDL 451. Investigation of the time-course reaction revealed that fatty acid was initially produced by lipase, followed by conversion into calcium-stearate. The fatty acid production rate was faster than that of the conversion into calcium-stearate at the beginning of the reaction. Alkaline pH, originating from the addition of calcium hydroxide, increased the converting reaction. This is the first report demonstrating that chemical production of calcium-stearate can be replaced by enzymatic reaction, thereby creating a cleaner process.
Department of Biological Engineering, Inha University, Incheon, Korea 402-751.
Hyang-Bok
HB
Lee
Jin-Soo
JS
Kwon
Young-Bum
YB
Kim
Eun-Ki
EK
Kim
D000818:Animals; D002417:Cattle; D005223:Fats / Q000737:chemistry* / Q000378:metabolism*; D006865:Hydrogenation / Q000187:drug effects; D006868:Hydrolysis / Q000187:drug effects; D008049:Lipase / Q000378:metabolism*; D008460:Meat; D013229:Stearic Acids / Q000737:chemistry / Q000378:metabolism*; D013997:Time Factors; D014867:Water / Q000494:pharmacology
D005223:Fats; D013229:Stearic Acids; D014867:Water; C031183:stearic acid; C013698:tallow; D008049:Lipase