Comparative assessment of normal and methoxypolyethylene glycol-modified murine red cells on swimming endurance and hippocampal injury in hypoxic mice.
BACKGROUND
Membrane grafting of methoxypolyethylene glycol (mPEG) provides a unique strategy in preventing the immunologic recognition in blood transfusion. mPEG-modified red blood cells (mPEG-RBCs) have acceptable in vitro properties and provide a useful solution to problems with clinical blood matching. The aim of this study was to demonstrate the physiologic normality of mPEG-RBCs in mice.


METHODS
Mouse RBCs were withdrawn via cardiac bleed and modified with 1.0 mmol per L mPEG with succinimidyl propionate linker. The fluorescein-labeled mPEG-RBCs were then transfused into recipient mice for in vivo survival analysis. At the same time, the exsanguine mouse model was produced, and mice were transfused with mPEG-RBCs. The effects of mPEG-RBC transfusion on the hemoglobin (Hb) level, swimming endurance capacity, and hypoxic-ischemic injury in hippocampal pyramidal cells of exsanguine mice were investigated.


RESULTS
mPEG-RBCs showed the same in vivo survival curve and t((1/2)) as those of untreated RBCs. Transfusion of mPEG-RBCs could elevate Hb level of exsanguine mice and improve their swimming endurance capacity, and histologic studies showed that mPEG-RBCs could also restore the hypoxic-ischemic injury of hippocampal pyramidal cells in exsanguine mice, which were similar with control RBCs. That is, mPEG-RBCs functioned in a similar fashion to untreated RBCs in exsanguine mice. Therefore, these results revealed that mPEG-RBCs had normal oxygen-carrying capacity.


CONCLUSIONS
In conclusion, the results confirmed that mPEG-RBCs could perform their in vivo function of carrying O(2) and improve some physiologic indexes of exsanguine mice, and the physiologic normality of mPEG-RBCs provides new findings for clinical use.
Tan
Yingxia
Y
Department of Blood Biochemistry and Molecular Biology Laboratory, Institute of Transfusion Medicine, Beijing, China.
Ji
Shouping
S
Li
Subo
S
Wang
Jiexi
J
Jin
Xiaopan
X
Zhang
Yangpei
Y
D000818:Animals; D002470:Cell Survival; D017707:Erythrocyte Transfusion / Q000379:methods*; D004912:Erythrocytes / Q000737:chemistry / Q000166:cytology / Q000502:physiology*; D005260:Female; D006454:Hemoglobins / Q000378:metabolism; D006624:Hippocampus / Q000473:pathology / Q000503:physiopathology; D000860:Hypoxia / Q000503:physiopathology; D051379:Mice; D011092:Polyethylene Glycols / Q000737:chemistry*; D013550:Swimming / Q000502:physiology
D006454:Hemoglobins; D011092:Polyethylene Glycols; C028210:monomethoxypolyethylene glycol