Cryopreserved human amniotic membrane for soft tissue repair in rats.
Fresh amniotic membrane has been used in medicine since 1910. The reconstruction of immunologic privileged ocular surfaces with cryopreserved amniotic membrane was introduced in the 1990s. The aim of this study was to analyze the use of cryopreserved human amniotic membrane (HAM) as a surgical patch in immunologic unprivileged anatomic sites. In part I of the investigation, the abdominal wall muscle of 36 rats was covered with mono- and multilayered HAM. After 3, 14, and 28 days, respectively, these grafts were evaluated macro- and microscopically. Multilayer samples displayed slower degradation and less inflammation compared with monolayer coverage. In part II of the study, abdominal wall closure with multilayer HAM and with polypropylene mesh was conducted in 20 rats. All rats showed sufficient closure after 21 days, but significantly lower intraabdominal adhesion formation was observed in the HAM rats. The results of this study might pave the way for the use of cryopreserved HAM as graft material in reconstructive surgery.
Department of Oral and Maxillofacial Plastic Surgery, University-Hospital, Knappschaftskrankenhaus Bochum-Langendreer, Bochum, Germany. marco.kesting@ruhr-uni-bochum.de
Marco Rainer
MR
Kesting
Denys John
DJ
Loeffelbein
Lars
L
Steinstraesser
Thomas
T
Muecke
Cedric
C
Demtroeder
Florian
F
Sommerer
Frank
F
Hoelzle
Klaus-Dietrich
KD
Wolff
D034861:Abdominal Wall / Q000473:pathology / Q000601:surgery*; D000650:Amnion / Q000473:pathology / Q000637:transplantation*; D000818:Animals; D003238:Connective Tissue / Q000473:pathology; D015925:Cryopreservation / Q000379:methods*; D004195:Disease Models, Animal; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D019651:Plastic Surgery Procedures / Q000379:methods*; D013524:Surgical Flaps; D013526:Surgical Mesh; D014183:Transplantation, Heterologous