HIV type 2 protease, reverse transcriptase, and envelope viral variation in the PBMC and genital tract of ARV-naive women in Senegal.
Unique viral variants and resistance mutations may occur in the genital tract of HIV-2 ARV-naive infected women. We sequenced and phylogenetically analyzed protease (PR), reverse transcriptase (RT), and envelope (ENV) from PBMC and genital tract samples from four ARV-naive women in Senegal. HIV-2 protease polymorphisms that predict HIV-1 protease inhibitor (PI) resistance were common. Two subjects had protease mutations (T77I and I64V) in genital tract samples that were not found in PBMCs. One subject had the HIV-2 reverse transcriptase M184I mutation in CVL DNA (but not PBMCs) that is known to confer 3TC/FTC resistance in HIV-2. In another subject, the reverse transcriptase A62V mutation was also found in CVL-RNA but not PBMCs. We found no significant difference in ENV variants between PBMCs and the genital tract. HIV-2 RT and PR mutations in the genital tract of ARV-naive females may have implications for transmitted HIV-2 resistance and ARV therapy.
Gottlieb
Geoffrey S
GS
Department of Medicine, School of Medicine, University of Washington, Seattle, Washington 98195, USA. gottlieb@u.washington.edu
Hawes
Stephen E
SE
Wong
Kim G
KG
Raugi
Dana N
DN
Agne
Habibatou D
HD
Critchlow
Cathy W
CW
Kiviat
Nancy B
NB
Sow
Papa Salif
PS
D000328:Adult; D000595:Amino Acid Sequence; D019380:Anti-HIV Agents / Q000627:therapeutic use; D002584:Cervix Uteri / Q000821:virology*; D024882:Drug Resistance, Viral / Q000235:genetics; D005260:Female; D015658:HIV Infections / Q000097:blood / Q000453:epidemiology*; D016333:HIV Protease / Q000235:genetics; D054303:HIV Reverse Transcriptase / Q000235:genetics; D015498:HIV-2 / Q000235:genetics*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000821:virology*; D016013:Likelihood Functions; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D011110:Polymorphism, Genetic; D012367:RNA, Viral / Q000032:analysis; D012675:Senegal / Q000453:epidemiology; D020539:Sequence Analysis, Protein / Q000706:statistics & numerical data; D014621:Vagina / Q000821:virology*; D054299:env Gene Products, Human Immunodeficiency Virus / Q000235:genetics
D019380:Anti-HIV Agents; D012367:RNA, Viral; D054299:env Gene Products, Human Immunodeficiency Virus; C072416:reverse transcriptase, Human immunodeficiency virus 2; D054303:HIV Reverse Transcriptase; D016333:HIV Protease; C065184:p16 protease, Human immunodeficiency virus 2