Amplifying small amounts of tumor DNA allows detection of DNA copy number abberations with array-CGH.
Array comparative genomic hybridization (aCGH) is a powerful tool to detect relative DNA copy number at a resolution limited only by the coverage of bacterial artificial chromosomes (BACs) used to print the genomic array. The amount of DNA needed to perform a reliable aCGH analysis has been a limiting factor, especially on minute tissue samples where limited DNA is available. Here we report a simple, highly sensitive and reliable aCGH method to analyze samples of no more than 1 ng genomic DNA. The speed and simplicity of the technique are ideal for studies on small clinical samples such as needle biopsies.
NorLux Neuro Oncology, Department of Biomedicine, University of Bergen, Bergen, Norway. lars.prestegarden@student.uib.no
Lars
L
Prestegarden
Anjan
A
Misra
Marcus L
ML
Ware
Ru-Fang
RF
Yeh
Rolf
R
Bjerkvig
Burt G
BG
Feuerstein
D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000032:analysis*; D018628:Gene Dosage; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis
D004273:DNA, Neoplasm