FAAP, a novel murine protein, is involved in cell adhesion through regulating vinculin-paxillin association.
Focal adhesion associated protein (FAAP), encoded by murine D10Wsu52e gene, is highly homologous to human HSPC117, which interacts with vinculin and talin. HeLa cells transfected with FAAP exhibited normal adhesion incorporation but showed impaired cell spreading, and restrained focal adhesion translocation. Moreover, FAAP facilitated vinculin-paxillin association, decreased interaction of paxillin-focal adhesion kinase and inhibited the phosphorylation of extracellular signal-regulated kinase. Together, these results suggest that FAAP, by virtue of modulating interaction of adhesion molecules, regulates cell adhesion dynamics.
The Key Laboratory of Cell Proliferation and Differentiation of Ministry of Education, The Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.
Jinsong
J
Hu
Junlin
J
Teng
Naizheng
N
Ding
Mei
M
He
Yuhui
Y
Sun
Albert Cheung Hoi
AC
Yu
Jianguo
J
Chen
D000818:Animals; D002448:Cell Adhesion / Q000235:genetics / Q000502:physiology*; D002465:Cell Movement / Q000502:physiology; D051417:Focal Adhesion Kinase 1 / Q000235:genetics / Q000502:physiology*; D005819:Genetic Markers; D006367:HeLa Cells / Q000166:cytology / Q000502:physiology; D006801:Humans; D051379:Mice; D051419:Paxillin / Q000502:physiology*; D014162:Transfection; D016596:Vinculin / Q000502:physiology*
D005819:Genetic Markers; D051419:Paxillin; D016596:Vinculin; D051417:Focal Adhesion Kinase 1