[Studies on the physiological response of Phytolacca americana to manganese toxicity by FTIR spectroscopy].
A technique based on Fourier transform infrared (FTIR) spectrometry was used to detect the physiological changes in chemical composition of Phytolacca americana under a wide range of Mn2+ treatments (0, 0.125, 0.25, 0.5 and 1 g x kg(-1)). Using the roots, stems and leaves of phytolacca americana, absorption bands corresponding to carbohydrates, ester and proteins varied differently. The absorption band heights at 3 000 and 2 916 cm(-1) of stem tissues rose firstly and then decreased, indicating the exudation and transporting situation of organic substances which served as organic osmotic contents to enhance manganese tolerance under low treatments; Meanwhile, the band heights at 2 922 cm(-1) (roots) and 1 606 cm(-1) (leaves) which have different tendency in accordance with Mn treatments showed that the capability to chelate Mn decreased under higher manganese concentration. In addition, the changes of the bands at 1 732 and 1 026 cm(-1) (roots), 1 028 cm(-1) (stems), 1 052 and 967 cm(-1) (leaves) differed from each other, suggesting that under the conditions of severe Mn stress the ex-oxidation of membrane lipid increased; Otherwise, the band heights at 1 375 cm(-1) increased firstly and then decreased, representing that the cell wall improved Mn resistance by increasing cation-exchange capacity (CEC). The result also implied that it is practical to apply FTIR to the research comparing the chemical differences of phytoaccumulators under metal treatments.
Key Laboratory of Botany, Zhejiang Normal University, Jinhua 321004, China.
Li-Min
LM
Ren
Ze-Feng
ZF
Cheng
Peng
P
Liu
Zhi-Gang
ZG
Li
D008345:Manganese / Q000633:toxicity*; D029604:Phytolacca americana / Q000737:chemistry* / Q000187:drug effects* / Q000254:growth & development; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D008345:Manganese