The yeast checkpoint kinase Dun1 downregulates DIN7 in the absence of DNA damage.
Yeast DIN7 is a DNA damage-inducible gene. Its expression is increased in the absence of Dun1, a DNA damage checkpoint kinase. We identified a DIN7 promoter region responsible for Dun1-mediated downregulation and found that DIN7 expression was not further increased in response to hydroxyurea in Deltadun1 cells. Thus DIN7 repression by Dun1 can be released upon DNA damage.
Chemical Genetics Laboratory, RIKEN Discovery Research Institute, Wako-shi, Saitama 351-0198, Japan.
Ayako
A
Yoshitani
Feng
F
Ling
Minoru
M
Yoshida
D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage / Q000235:genetics; D015536:Down-Regulation; D005090:Exodeoxyribonucleases / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D011401:Promoter Regions, Genetic / Q000235:genetics; D011494:Protein Kinases / Q000235:genetics / Q000378:metabolism*; D017346:Protein Serine-Threonine Kinases; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
D018797:Cell Cycle Proteins; D029701:Saccharomyces cerevisiae Proteins; D011494:Protein Kinases; C104169:DUN1 protein, S cerevisiae; D017346:Protein Serine-Threonine Kinases; C105071:DIN7 protein, S cerevisiae; D005090:Exodeoxyribonucleases