Development of PCR-based SCAR and CAPS markers linked to beta-glucan and protein content QTL regions in oat.
A key breeding objective in oat (Avena sativa L.) is cultivars with high and low beta-glucan content. In a targeted strategy to develop PCR-based markers linked to published beta-glucan content quantitative trait loci (QTLs) regions, 15 random amplified polymorphic DNA (RAPD) fragments were cloned and their sequences used to design sequence-characterized amplified region (SCAR) and cleaved amplified polymorphic sequence (CAPS) primers. The 13 derived SCAR markers and 2 derived CAPS markers were mapped on either the 'Kanota' x 'Ogle' (KO) or the 'Terra' x 'Marion' (TM) oat reference map. In addition, 3 previously reported SCAR markers were characterized further. Ten SCAR markers and one CAPS marker were associated with beta-glucan QTL regions and many of these are also associated with QTLs for protein content or other traits. These markers have the potential to help define homologous and homologous relationships in oat and investigate the complex genetics of beta-glucan and protein content.
Orr
Winson
W
Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Central Experimental Farm, 960 Carling Avenue, Ottawa, ON K1A0C6, Canada.
Molnar
Stephen J
SJ
D018554:Avena / Q000235:genetics*; D002874:Chromosome Mapping; D018744:DNA, Plant / Q000235:genetics; D005819:Genetic Markers / Q000235:genetics*; D010940:Plant Proteins / Q000378:metabolism*; D016133:Polymerase Chain Reaction / Q000379:methods*; D040641:Quantitative Trait Loci; D019105:Random Amplified Polymorphic DNA Technique; D047071:beta-Glucans / Q000378:metabolism*
D018744:DNA, Plant; D005819:Genetic Markers; D010940:Plant Proteins; D047071:beta-Glucans