[Determination of rubrofusarin gentiobioside in Cassia obtusifolia by HPLC].
OBJECTIVE
To establish a method for determining the content of rubrofusarin gentiobioside in Cassia obtusifolia.


METHODS
The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, acetonitrile-THF-1% acetic acid (18: 3:79) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees 2 and detection wavelength at 278 nm.


RESULTS
A good linearity was obtained from 0.1-0.5 microg with r = 0.999 9 for rubrofusarin gentiobioside. The average recovery was 101.1%, and RSD was 2.23% (n = 5).


CONCLUSIONS
The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.
Tang
Li-Ying
LY
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang
Zhu-Ju
ZJ
Wu
Qiu-Ping
QP
He
Yan
Y
Huang
Lu-Qi
LQ
D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000032:analysis* / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results
D002867:Chromones; D004365:Drugs, Chinese Herbal; D005960:Glucosides; C119595:rubrofusarin gentiobioside