Collision-induced dissociation of three groups of hydroxamate siderophores: ferrioxamines, ferrichromes and coprogens/fusigens.
The behaviour of a series of hydroxamate siderophores--microbially produced iron complexes - was investigated using electrospray ionisation mass spectrometry (ESI-MS). Three groups of iron hydroxamate siderophores, namely the ferrioxamines, ferrichromes and coprogens/fusigens, were separated by high-performance liquid chromatography (HPLC) prior to ESI and MS(2) fragmentation. For the majority of the siderophores, both protonated molecules and sodium adducts were observed. The most abundant ion was selected for collision-induced fragmentation. Potential fragmentation mechanisms are postulated and discussed. Fragmentation patterns differed between siderophore groups; however, common fragmentation patterns were observed for siderophore ions within the groups examined. Cleavage frequently occurred at carbon-nitrogen or carbon-oxygen bonds. Fragmentation of the ions also involved cleavage of iron-oxygen bonds and transfer of the charge to iron.
School of Ocean and Earth Science, National Oceanography Centre, University of Southampton, Southampton SO14 3ZH, UK.
Edward
E
Mawji
Martha
M
Gledhill
Paul J
PJ
Worsfold
Eric P
EP
Achterberg
D005290:Ferric Compounds / Q000737:chemistry*; D005291:Ferrichrome / Q000737:chemistry*; D006877:Hydroxamic Acids / Q000737:chemistry*; D013058:Mass Spectrometry; D015394:Molecular Structure; D008970:Molecular Weight; D012015:Reference Standards; D015203:Reproducibility of Results; D017262:Siderophores / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D005290:Ferric Compounds; D006877:Hydroxamic Acids; D017262:Siderophores; C074579:iron (III) hydroxamate; D005291:Ferrichrome; C010966:fusigen; C010896:coprogen