Iron oxide-loaded liposomes for MR imaging.
In this study a liposome cell labeling system was developed for non-target-specific labeling of glioma cells with superparamagnetic iron oxide nanoparticles for magnetic resonance imaging (MRI). A high non-target-specific uptake is ideal for in vitro labeling of cells and subsequently for cell tracking and visualization of phagocytic cells in vivo. The preparation of iron oxide-loaded liposomes was optimized and the biological properties of the liposomes were investigated. Cytotoxicity and cell viability were examined and showed limited cytotoxic effects. Non-target-specific labeling of glioma cells in vitro for subsequent specific labeling of molecules for MR imaging was tested by T2*-weighted MRI at 3T. The glioma cells showed a strong initial uptake of the iron oxide liposomes and the uptake was not saturable within 24 h exposure. The uptake of liposomes was superior to non-coated magnetite nanoparticles. Using PEG-ylated liposomes, the non-specific uptake could be decreased fundamentally (86% lower) in comparison to conventional liposomes. Furthermore, the ability of liposomes as contrast agents for MR imaging was investigated. Cells labeled with iron oxide nanoparticles by treatment with liposomes showed a negative contrast in MRI and consequently successful cellular labeling. Thus, iron oxide-loaded liposomes are well suited for non-target-specific cell labeling for MR imaging.
Department of Anatomy, Christian-Albrechts-University zu Kiel, Germany.
Manuela
M
Meincke
Thomas
T
Schlorf
Elke
E
Kossel
Olav
O
Jansen
Claus-Christian
CC
Glueer
Rolf
R
Mentlein
D045744:Cell Line, Tumor; D002470:Cell Survival; D005290:Ferric Compounds / Q000493:pharmacokinetics; D005910:Glioma / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D007202:Indicators and Reagents; D008081:Liposomes; D008279:Magnetic Resonance Imaging / Q000379:methods*; D010587:Phagocytosis; D010713:Phosphatidylcholines
D005290:Ferric Compounds; D007202:Indicators and Reagents; D008081:Liposomes; D010713:Phosphatidylcholines; C000499:ferric oxide