Fate and impact on microorganisms of rice allelochemicals in paddy soil.
Allelopathic rice can release allelochemicals from roots to inhibit neighboring plant species, but little is currently known about their fate and impact on microorganisms in paddy soil. This study showed that allelopathic rice PI312777 released much higher concentrations of allelochemical (5,7,4'-trihydroxy-3',5'-dimethoxyflavone) than non-allelopathic rice Liaojing-9 in field. When quantitative 5,7,4'-trihydroxy-3',5'-dimethoxyflavone was added into soil, flavone gave a short half-life of 18.27 +/- 2.32 h (r(2) = 0.94) and could easily be degraded into benzoic acid. Benzoic acid with a half-life of 29.99 +/- 2.19 h (r(2) = 0.96) was more resistant toward degradation in paddy soil. Furthermore, both the culturable microbial population and the entire microbial community structure of soil incubated with flavone and benzoic acid were evaluated using the soil dilution plate method and phospholipid fatty acid (PLFA) analysis, respectively. It appeared from the results that flavone could reduce microorganisms especially for fungi present in paddy soil, while benzoic acid could induce a higher response for soil microorganisms especially for bacteria. Consequently, flavone would be responsible for the dynamics of soil microorganisms during the early period, and any observed effect during the late period would be very likely due to its degradation product benzoic acid rather than flavone itself. These results suggested that allelopathic rice varieties could modify soil microorganisms to their advantage through the release of allelochemicals. The concentration and fate of discriminating 5,7,4'-trihydroxy-3',5'-dimethoxyflavone between allelopathic and non-alleloparhic varieties tested in rice soil would result in the different patterns of microbial population and community structure in paddy ecosystems.
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. kongch@iae.ac.cn
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D001419:Bacteria / Q000187:drug effects*; D001673:Biodegradation, Environmental; D018533:Biomass; D017753:Ecosystem; D005658:Fungi / Q000187:drug effects*; D012275:Oryza / Q000378:metabolism*; D010675:Pheromones / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D018517:Plant Roots / Q000378:metabolism; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D010675:Pheromones; D012987:Soil