Calcium spatial distribution in aerobic granules and its effects on granule structure, strength and bioactivity.
Calcium-rich aerobic granules were cultivated after 3-month operation. The chemical form and spatial distribution of calcium in the granules and their physicochemical characteristics were explored. Examination with a scanning electron microscope combined energy dispersive X-ray detector (SEM-EDX) shows that Ca was mainly accumulated in the core of the granules. CaCO(3) was found to be the main calcium precipitate in the granules. The fluorescent in situ hybridization (FISH) analysis shows that the cells were crowded in the outer layer and gathered in clusters. Compared with the granules without Ca accumulation, the Ca-rich granules had more rigid structure and a higher strength. However, their specific oxygen uptake rate (SOUR) reduced after the Ca accumulation inside them. Comparison between the SOUR values of the granules with and without Ca accumulation suggests that Ca accumulated in the aerobic granules might have a negative effect on their bioactivity.
Department of Chemistry, University of Science and Technology of China, Hefei, China.
Ting-Ting
TT
Ren
Li
L
Liu
Guo-Ping
GP
Sheng
Xian-Wei
XW
Liu
Han-Qing
HQ
Yu
Ming-Chuan
MC
Zhang
Jian-Rong
JR
Zhu
D000332:Aerobiosis; D002118:Calcium / Q000032:analysis*; D007477:Ions / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D013314:Stress, Mechanical
D007477:Ions; D002118:Calcium