Association of E1AF mRNA expression with tumor progression and matrilysin in human rectal cancer.
OBJECTIVE
To examine E1AF mRNA expression and to determine whether it is correlated with tumor progression and matrilysin in human rectal cancer.


METHODS
Real-time RT-PCR was used to determine E1AF and matrilysin expression in 100 matched rectal cancers and normal tissues.


RESULTS
Among the 100 rectal cancers, 69 cases of E1AF mRNA overexpression were observed. E1AF mRNA overexpression correlated well with matrilysin. In carcinomas, E1AF mRNA overexpression correlated significantly with depth of invasion, lymph node metastasis, venous involvement and advanced pTNM stage.


CONCLUSIONS
E1AF was correlated significantly with tumor progression of human rectal cancer and may be an important factor in rectal cancer progression.
Liu
Hai-Yi
HY
Institute of Digestive Surgery and Organ Microcirculation, Department of Gastrointestinal Surgery, Sichuan University, Chengdu, China.
Zhou
Bin
B
Wang
Ling
L
Li
Yuan
Y
Zhou
Zong-Guang
ZG
Sun
Xiao-Feng
XF
Xu
Bing
B
Zeng
Yu-Jian
YJ
Song
Jun-Min
JM
Luo
Hong-Zhi
HZ
Yang
Lie
L
D017500:Adenovirus E1A Proteins / Q000235:genetics*; D000368:Aged; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D012007:Rectum / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017500:Adenovirus E1A Proteins; C079853:ETV4 protein, human; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7