Mitochondria in energy-limited states: mechanisms that blunt the signaling of cell death.
Cellular conditions experienced during energy-limited states--elevated calcium, shifts in cellular adenylate status, compromised mitochondrial membrane potential--are precisely those that trigger, at least in mammals, the mitochondrion to initiate opening of the permeability transition pore, to assemble additional protein release channels, and to release pro-apoptotic factors. These pro-apototic factors in turn activate initiator and executer caspases. How is activation of mitochondria-based pathways for the signaling of apoptotic and necrotic cell death avoided under conditions of hypoxia, anoxia, diapause, estivation and anhydrobiosis? Functional trade-offs in environmental tolerance may have occurred in parallel with the evolution of diversified pathways for the signaling of cell death in eukaryotic organisms. Embryos of the brine shrimp, Artemia franciscana, survive extended periods of anoxia and diapause, and evidence indicates that opening of the mitochondrial permeability transition pore and release of cytochrome c (cyt-c) do not occur. Further, caspase activation in this crustacean is not dependent on cyt-c. Its caspases display regulation by nucleotides that is consistent with ;applying the brakes' to cell death during energy limitation. Unraveling the mechanisms by which organisms in extreme environments avoid cell death may suggest possible interventions during disease states and biostabilization of mammalian cells.
Hand
Steven C
SC
Division of Cellular, Developmental and Integrative Biology, Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA. shand@lsu.edu
Menze
Michael A
MA
D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001156:Artemia / Q000502:physiology*; D016923:Cell Death / Q000502:physiology*; D015687:Cell Hypoxia / Q000502:physiology; D004734:Energy Metabolism / Q000502:physiology*; D008928:Mitochondria / Q000502:physiology*; D051336:Mitochondrial Membranes / Q000378:metabolism; D008954:Models, Biological; D010539:Permeability; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism; D015398:Signal Transduction / Q000502:physiology*
D019253:Proto-Oncogene Proteins c-bcl-2; D000255:Adenosine Triphosphate