[Pathway analysis of modulation on gene expression in murine lymphocytes by Lactobacillus peptidoglycan].
OBJECTIVE
To investigate the mechanism that Lactobacillus peptidoglycan modulates mice immune response.


METHODS
BALB/c mice were administrated (i.p.) with Lactobacillus peptidoglycan once or three times. Peritoneal macrophages and spleen lymphocytes were isolated for gene expression analysis using gene array. PathwayExplorer and GeneMAPP were used to explore significant pathway in database.


RESULTS
The analysis resulted in five significant pathways: cytokine-cytokine receptor interaction, T helper cell surface molecules, ribosomal proteins, inflammatory response pathway and mm heme biosynthesis.


CONCLUSIONS
Lactobacillus peptidoglycan induced expression of considerable genes, which related to protective immune response and activation of Th cells. The induced immune response might be Th1 type.
Southern Yangtze University, Wuxi, China. sunj@jiangnan.edu.cn
Jin
J
Sun
Gui-fang
GF
Chang
Guo-wei
GW
LE
Yong-hui
YH
Shi
D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007778:Lactobacillus / Q000276:immunology*; D008214:Lymphocytes / Q000276:immunology*; D017737:Macrophages, Peritoneal / Q000276:immunology*; D008297:Male; D051379:Mice / Q000235:genetics* / Q000276:immunology; D008807:Mice, Inbred BALB C; D010457:Peptidoglycan / Q000008:administration & dosage* / Q000276:immunology; D011897:Random Allocation
D016207:Cytokines; D010457:Peptidoglycan