HSV-1 ICP27 suppresses NF-kappaB activity by stabilizing IkappaBalpha.
Nuclear factor kappaB (NF-kappaB) is associated with the transcriptional activation of genes encoding chemokines, adhesion molecules, cytokines, and anti-apoptotic proteins, which are key components in immune responses and viral infection. Many viruses modulate NF-kappaB through numerous viral gene products to allow productive infections and immune escape. Here we report that herpes simplex virus-1 infected cell protein 27 (HSV-1 ICP27), an immediate early protein of HSV-1, represses NF-kappaB activity through binding to inhibitor of kappaB (IkappaBalpha), blocking phosphorylation and ubiquitination of IkappaBalpha, and stabilizing IkappaBalpha. These data may explain how NF-kappaB activity is regulated by ICP27 to escape immune responses during the very early period of HSV-1 infection.
Kim
Jin Chul
JC
Department of Microbiology, School of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Republic of Korea.
Lee
Soo Yun
SY
Kim
Sang Young
SY
Kim
Jeong Ki
JK
Kim
Hye Jin
HJ
Lee
Hee Min
HM
Choi
Mi Sun
MS
Min
Jung Sun
JS
Kim
Mi Jee
MJ
Choi
Hyang Soon
HS
Ahn
Jeong Keun
JK
D000818:Animals; D002460:Cell Line; D002522:Chlorocebus aethiops; D020672:I-kappa B Proteins / Q000378:metabolism*; D017874:Immediate-Early Proteins / Q000378:metabolism*; D000072000:NF-KappaB Inhibitor alpha; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010766:Phosphorylation; D054875:Ubiquitination; D014709:Vero Cells
D020672:I-kappa B Proteins; C057120:ICP27 protein, human herpesvirus 1; D017874:Immediate-Early Proteins; D016328:NF-kappa B; C000606204:NFKBIA protein, human; D000072000:NF-KappaB Inhibitor alpha