Long-term expansion of effector/memory Vdelta2-gammadelta T cells is a specific blood signature of CMV infection.
The ability of human gammadelta T cells to develop immunologic memory is still a matter of debate. We previously demonstrated the involvement of Vdelta2- gammadelta T lymphocytes in the response of immunosuppressed organ recipients to cytomegalovirus (CMV). Here, we demonstrate their ability to mount an adaptive immune response to CMV in immunocompetent subjects. Vdelta2- gammadelta T-cell peripheral blood numbers, repertoire restriction, and cytotoxicity against CMV-infected fibroblasts were markedly increased in CMV-seropositive, compared with CMV-seronegative, healthy persons. Whereas Vdelta2- gammadelta T cells were found as naive cells in CMV- patients, they virtually all exhibited the cytotoxic effector/memory phenotype in CMV+ patients, which is also observed in transplanted patients challenged with CMV. This long-term complete remodeling of the Vdelta2- gammadelta T-cell population by CMV predicts their ability to exhibit an adaptive anti-CMV immune response. Consistent with this, we observed that the secondary response to CMV was associated with a faster gammadelta T-cell expansion and a better resolution of infection than the primary response. In conclusion, the increased level of effector-memory Vdelta2- gammadelta T cells in the peripheral blood is a specific signature of an adaptive immune response to CMV infection of both immunocompetent and immunosuppressed patients.
Université Bordeaux 2, Bordeaux, France.
Vincent
V
Pitard
David
D
Roumanes
Xavier
X
Lafarge
Lionel
L
Couzi
Isabelle
I
Garrigue
Marie-Edith
ME
Lafon
Pierre
P
Merville
Jean-François
JF
Moreau
Julie
J
Déchanet-Merville
D049109:Cell Proliferation; D002478:Cells, Cultured; D003586:Cytomegalovirus Infections / Q000097:blood / Q000276:immunology*; D003602:Cytotoxicity, Immunologic; D006801:Humans; D007111:Immunity, Cellular; D007156:Immunologic Memory; D018655:Lymphocyte Count; D016692:Receptors, Antigen, T-Cell, gamma-delta; D013601:T-Lymphocytes / Q000276:immunology* / Q000821:virology
D016692:Receptors, Antigen, T-Cell, gamma-delta