Analysis of interactions between carbohydrates and proteins using fluorescent labeling and SDS-PAGE.
A rapid method for the analysis of carbohydrate-protein interactions by using fluorescent labeling and SDS-PAGE was developed. The N-acetyl-beta-D-glucosamine-WGA complex and alpha-D-mannose-Con A complex were labeled with 8-aminonaphthalene-1,3,6-trisulfonate (ANTS). The protein band displaying fluorescence with ultraviolet illumination was seen after SDS-PAGE.
Huang
Gang L
GL
College of Chemistry, Chongqing Normal University, Chongqing 400047, China. huangdoctor226@163.com
Wong
Kongliang
K
D000117:Acetylglucosamine / Q000378:metabolism; D002241:Carbohydrates / Q000302:isolation & purification; D003208:Concanavalin A / Q000378:metabolism; D004591:Electrophoresis, Polyacrylamide Gel; D005456:Fluorescent Dyes; D011506:Proteins / Q000302:isolation & purification / Q000378:metabolism*; D014909:Wheat Germ Agglutinins / Q000378:metabolism
D002241:Carbohydrates; D005456:Fluorescent Dyes; D011506:Proteins; D014909:Wheat Germ Agglutinins; D003208:Concanavalin A; D000117:Acetylglucosamine