[Effect of Capparis spinosa on fibroblast proliferation and type I collagen production in progressive systemic sclerosis].
OBJECTIVE
To investigate the effects of ethanolic extract from Capparis spinosa (ECS) on the fibroblast proliferation and type I collagen production in normal and progressive systemic sclerosis (PSS).


METHODS
Cellular activity was determined by the MTT method. Apoptosis was detected by flow cytometry analysis of Annexin V-stained cells. The expression levels of type I collagen messenger RNA and protein were analyzed by RT-PCR and western blot analysis.


RESULTS
ECS could significantly inhibit the proliferation of fibroblast and reduced the expression of alpha2 (I) collagen mRNA and type I collagen protein in PSS in a dose-and time-dependent manner. ECS did not affect the proliferation of fibroblast and expression of type I collagen mRNA and protein in normal human. ECS could counteract the harmful effects on fibroblast by H2O2.


CONCLUSIONS
ECS can effectively inhibit the fibroblast proliferation and type I collagen production in PSS.
Cao
Yue-Lan
YL
Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310009, China. CYL123321@sohu.com
Li
Xin
X
Zheng
Min
M
D031484:Capparis / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D024042:Collagen Type I / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D045743:Scleroderma, Diffuse / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology
D024042:Collagen Type I; D004365:Drugs, Chinese Herbal