Effects of probe contact pressure on in vivo optical spectroscopy.
The short- and long-term effects of probe contact pressure on in vivo diffuse reflectance and fluorescence spectroscopy were investigated using an animal model. Elevation in probe contact pressure induced major profile alterations in the diffuse reflectance spectra between 400 and 650 nm, and led to significant intensity increases in the fluorescence spectra. The pressure threshold that was required to induce statistically significant spectral alterations was dependent upon the type of tissue. The observed spectral alterations may be attributed to decreases in local blood volume, blood oxygenation, and tissue metabolism, resulting from high probe contact pressure.
Ti
Yalin
Y
Department of Biomedical Engineering, Florida International University, Miami, Florida 33199, USA.
Lin
Wei-Chiang
WC
D016477:Artifacts; D006321:Heart / Q000502:physiology*; D006801:Humans; D008099:Liver / Q000502:physiology*; D055095:Optics and Photonics; D010783:Photometry / Q000379:methods*; D011312:Pressure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods*