An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in Radix Aconiti Lateralis Preparata (Fuzi, aconite roots) and its products.
BACKGROUND
Benzoylmesaconine (BMA) is the main Aconitum alkaloid in Radix Aconiti Lateralis Preparata (Fuzi, aconite roots) with potent pharmacological activities, such as analgesia and anti-inflammation. The present study developed a simple and reliable method using BMA as a marker compound for the quality control of processed aconite roots and their products.


METHODS
After extraction, a high-performance liquid chromatography (HPLC) determination of BMA was conducted on a RP-C18 column by gradient elution with acetonitrile and aqueous phase, containing 0.1% phosphoric acid adjusted with triethylamine to pH 3.0.


RESULTS
A distinct peak profile was obtained and separation of BMA was achieved. Method validation showed that the relative standard deviations (RSDs) of the precision of BMA in all intra-day and inter-day assays were less than 1.36%, and that the average recovery rate was 96.95%. Quantitative analysis of BMA showed that the content of BMA varied significantly in processed aconite roots and their products.


CONCLUSIONS
This HPLC method using BMA as a marker compound is applicable to the quality control of processed aconite roots and their products.
Xie
Ying
Y
School of Chinese Medicine, Hong Kong Baptist University, Hong Kong SAR, PR China. 04415930@hkbu.edu.hk
Zhou
Hua
H
Wong
Yuen Fan
YF
Liu
Zhongqiu
Z
Xu
Hongxi
H
Jiang
Zhihong
Z
Liu
Liang
L