An ultraviolet melting study of the stability of the DNA double helix in the NaDNA-bipyridyl-(ethylenediamine)platintum(II) complex.
Complexes of NaDNA with the bipyridyl-(ethylenediamine)platintum(II) (abbreviated [(bipy)Pt(en)]2+) molecular ion have been studied in solution via ultraviolet absorption experiments at 260 nm between 50 and 90 degrees C. These measurements, performed as a function of the molar ratio of the [(bipy)Pt(en)]2+ complex to DNA base pairs, show that the stability of the DNA double helix is increased by the formation of the DNA.[(bipy)Pt(en)]2+ complex: at a molar ratio of 0.33, the temperature at which the DNA double helix separates into two single strands is increased by about 15 degrees C.
Department of Physics and Astronomy, University of Toledo, Toledo, OH 43606, USA.
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Szabó
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Lee
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D018736:DNA Adducts / Q000737:chemistry* / Q000528:radiation effects*; D009944:Organoplatinum Compounds / Q000737:chemistry* / Q000528:radiation effects*; D013816:Thermodynamics; D014466:Ultraviolet Rays
D018736:DNA Adducts; C460911:DNA-bipyridyl-(ethylenediamine)platinum(II); D009944:Organoplatinum Compounds; D015082:2,2'-Dipyridyl; D004247:DNA