Changes in the expression of interleukin-1beta and lipopolysaccharide-induced TNF factor in the oviduct of laying hens in response to artificial insemination.
The aim of this study was to determine the physiological significance of interleukin-1beta (IL1B) and lipopolysaccharide-induced TNF factor (LITAF) in the fate of sperm in the oviduct of laying hens after artificial insemination (AI). Laying hens were inseminated with fresh semen, PBS or seminal plasma and tissues from different oviductal segments were collected to observe the general histology, changes in the mRNA expression of IL1B and LITAF and the localization of positive cells expressing immunoreactive IL1B (irIL1B). Semi-quantitative RT-PCR was used to observe the changes in mRNA expression of these molecules in the infundibulum, uterus, utero-vaginal junction (UVJ), and vagina after insemination. Intact sperm in the lumen and between the primary or secondary folds of the vagina were found until 6 h after insemination but were degraded at 12 h. The mRNA expression of IL1B and LITAF was significantly increased in the vagina until 6 h after AI but remained unchanged in the other oviductal segments. In the tissue of the vagina and UVJ, irIL1B was localized in the mucosal stroma. The number of irIL1B-positive cells was increased in the vagina but almost unchanged in UVJ after insemination with semen. Significant changes were not observed in the mRNA expression and irIL1B-positive cells in the vagina after PBS or seminal plasma insemination. The increase of IL1B and LITAF in the vagina may lead to sperm degradation and elimination by cilia of surface epithelium, whereas their lower levels in UVJ may permit sperm to survive in sperm storage tubules.
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
Shubash Chandra
SC
Das
Naoki
N
Isobe
Yukinori
Y
Yoshimura
D000818:Animals; D002470:Cell Survival; D002645:Chickens / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D007150:Immunohistochemistry; D007315:Insemination, Artificial; D053583:Interleukin-1beta / Q000032:analysis / Q000235:genetics*; D008297:Male; D010057:Oviducts / Q000276:immunology*; D012333:RNA, Messenger / Q000032:analysis; D018124:Receptors, Tumor Necrosis Factor / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013094:Spermatozoa / Q000737:chemistry / Q000166:cytology; D015291:Transforming Growth Factors / Q000235:genetics*; D014599:Uterus / Q000276:immunology; D014621:Vagina / Q000276:immunology
D053583:Interleukin-1beta; D012333:RNA, Messenger; D018124:Receptors, Tumor Necrosis Factor; D015291:Transforming Growth Factors