In situ activation of antigen-specific CD8+ T cells in the presence of antigen in organotypic brain slices.
The activation of Ag-specific T cells locally in the CNS could potentially contribute to the development of immune-mediated brain diseases. We addressed whether Ag-specific T cells could be stimulated in the CNS in the absence of peripheral lymphoid tissues by analyzing Ag-specific T cell responses in organotypic brain slice cultures. Organotypic brain slice cultures were established 1 h after intracerebral OVA Ag microinjection. We showed that when OVA-specific CD8(+) T cells were added to Ag-containing brain slices, these cells became activated and migrated into the brain to the sites of their specific Ags. This activation of OVA-specific T cells was abrogated by the deletion of CD11c(+) cells from the brain slices of the donor mice. These data suggest that brain-resident CD11c(+) cells stimulate Ag-specific naive CD8(+) T cells locally in the CNS and may contribute to immune responses in the brain.
Department of Pathology and Laboratory Medicine, University of Wisconsin Medical School, University of Wisconsin, Madison, WI 53706, USA.
Changying
C
Ling
Yakov I
YI
Verbny
Matthew I
MI
Banks
Matyas
M
Sandor
Zsuzsanna
Z
Fabry
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D039521:CD11c Antigen / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D049109:Cell Proliferation; D002474:Cell-Free System / Q000276:immunology / Q000378:metabolism; D018920:Coculture Techniques; D018984:Epitopes, T-Lymphocyte / Q000096:biosynthesis / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009924:Organ Culture Techniques; D009928:Organ Specificity / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology
D039521:CD11c Antigen; D018984:Epitopes, T-Lymphocyte; D010047:Ovalbumin