The intron-containing genome of the lytic Pseudomonas phage LUZ24 resembles the temperate phage PaP3.
The virulent Pseudomonas aeruginosa bacteriophage LUZ24 (45,625 bp) was isolated from hospital sewage. It belongs to the family of the Podoviridae, and carries a bidirectionally transcribed dsDNA genome delineated by two direct terminal repeats of 184 bp. In vitro transcriptional analysis identified seven sigma(70) promoters, revealing a bias towards stronger promoter strength in the late genomic region. Reverse transcription demonstrated in vivo splicing of a 668 bp Group I intron embedded inside the DNA polymerase gene. Using mass spectrometry, nine structural proteins were identified as part of the phage particle. The lytic characteristics of LUZ24 are evaluated against its genomic content, which displays an overall 71% sequence similarity to the temperate phage PaP3.
Division of Gene Technology, Katholieke Universiteit Leuven, Kasteelpark Arenberg 21, Leuven, B-3001, Belgium.
Pieter-Jan
PJ
Ceyssens
Kirsten
K
Hertveldt
Hans-W
HW
Ackermann
Jean-Paul
JP
Noben
Mekonnen
M
Demeke
Guido
G
Volckaert
Rob
R
Lavigne
D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005814:Genes, Viral; D016679:Genome, Viral; D007438:Introns / Q000235:genetics*; D016366:Open Reading Frames; D017105:Pseudomonas Phages / Q000145:classification / Q000235:genetics* / Q000502:physiology; D014158:Transcription, Genetic / Q000502:physiology*
D004279:DNA, Viral