Cyclosporine action on kidneys of rats submitted to normothermic ischaemia and reperfusion.
OBJECTIVE
To verify if rat kidneys lesioned by ischaemia followed by reperfusion are affected by cyclosporine A (CsA).


METHODS
Male Wistar rats were randomly divided into three groups, control (GS) and experimental (G1 and G2). G1 was subdivided in two: G1A composed of animals submitted to 60 minutes ischaemia and G1C with the same ischaemic procedure associated to 20 mg/kg/day CsA. Group G2 was subdivided and treated in the same way as G1 except that ischaemia was applied only for 40 minutes. Clamping the left renal artery followed by right side nephrectomy induced kidney ischaemia. Serum urea and creatinine were quantified on the day of surgery (D0) and in the following day (D1). Twenty four hours after reperfusion the left kidney was removed and histologically analyzed.


RESULTS
Group GS had normal values for urea and creatinine both on D0 and D1 and did not show structural alterations. Renal function was not significantly different when G2C was compared to GS (p>0.05). Tissue lesions were smaller in G2C than in the other groups.


CONCLUSIONS
Renal function was protected by CsA, which also reduced tissue lesions in the kidneys of rats submitted to 40 minutes ischaemia.
Department of Surgery and Anatomy, Ribeirão Preto Faculty of Medicine, University of São Paulo, SP, Brazil. ajcologn@fmrp.usp.br
Adauto José
AJ
Cologna
Lucy Vieira da Silva
LV
Lima
Silvio
S
Tucci
Haylton Jorge
HJ
Suaid
Rodolfo Borges
RB
Reis
Luis Fernando
LF
Tirapelli
Antônio Antunes
AA
Rodrigues
Antonio Carlos Pereira
AC
Martins
D000818:Animals; D015415:Biomarkers / Q000097:blood; D003404:Creatinine / Q000097:blood; D016572:Cyclosporine / Q000494:pharmacology*; D004195:Disease Models, Animal; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D007511:Ischemia / Q000150:complications* / Q000517:prevention & control; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects* / Q000503:physiopathology; D008297:Male; D009392:Nephrectomy; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D013997:Time Factors; D014508:Urea / Q000097:blood
D015415:Biomarkers; D007166:Immunosuppressive Agents; D016572:Cyclosporine; D014508:Urea; D003404:Creatinine