Enhanced resistance against systemic Candida albicans infection in mice treated with C. albicans DNA.
In this study, double-stranded Candida albicans DNA was administered in systemic C. albicans infection in at dose of 20 microg per mouse at 4, 5 and 6 weeks of age. The level of IL-12 in serum was elevated as a result of yeast DNA treatment and correlated with lower mortality and decreased kidney and liver injury. Macrophage activation was demonstrated by an increase of nitric oxide (NO) and IL-12 production. These effects were Janus activation kinases (JAK)/signal transducer and activator of transcription (STAT) dependent as they were inhibited by selective JAK inhibitor tyrphostin AG-490. DNA influenced adaptive immune response through elevation of anti-Candida IgG antibody production in systemic C. albicans infection. Thus, C. albicans DNA augmented innate and adaptive immune responses against the pathogen.
Department of Immunology, Institute of Microbiology, Sofia, Bulgaria.
Petya
P
Dimitrova
Martin
M
Yordanov
Svetla
S
Danova
Nina
N
Ivanovska
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D002176:Candida albicans / Q000235:genetics / Q000276:immunology*; D002177:Candidiasis / Q000276:immunology* / Q000628:therapy*; D015169:Colony Count, Microbial; D004271:DNA, Fungal / Q000008:administration & dosage / Q000627:therapeutic use*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D007074:Immunoglobulin G / Q000097:blood; D018664:Interleukin-12 / Q000097:blood; D053612:Janus Kinases / Q000037:antagonists & inhibitors / Q000276:immunology; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008264:Macrophages / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000096:biosynthesis; D050791:STAT Transcription Factors / Q000276:immunology; D016019:Survival Analysis; D020032:Tyrphostins / Q000494:pharmacology
D000908:Antibodies, Fungal; D004271:DNA, Fungal; D004791:Enzyme Inhibitors; D007074:Immunoglobulin G; D050791:STAT Transcription Factors; D020032:Tyrphostins; C095512:alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; D018664:Interleukin-12; D009569:Nitric Oxide; D053612:Janus Kinases