Structural genomics of minimal organisms: pipeline and results.
The initial objective of the Berkeley Structural Genomics Center was to obtain a near complete three-dimensional (3D) structural information of all soluble proteins of two minimal organisms, closely related pathogens Mycoplasma genitalium and M. pneumoniae. The former has fewer than 500 genes and the latter has fewer than 700 genes. A semiautomated structural genomics pipeline was set up from target selection, cloning, expression, purification, and ultimately structural determination. At the time of this writing, structural information of more than 93% of all soluble proteins of M. genitalium is avail able. This chapter summarizes the approaches taken by the authors' center.
Kim
Sung-Hou
SH
Berkeley Structural Genomics Center, Lawrence Berkeley National Laboratory, and Department of Chemistry, University of California, Berkeley, CA, USA.
Shin
Dong-Hae
DH
Kim
Rosalind
R
Adams
Paul
P
Chandonia
John-Marc
JM
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D003001:Cloning, Molecular; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D016680:Genome, Bacterial / Q000235:genetics*; D023281:Genomics / Q000379:methods*; D008958:Models, Molecular; D045704:Mycoplasma genitalium / Q000235:genetics*; D009177:Mycoplasma pneumoniae / Q000235:genetics*; D017510:Protein Folding
D001426:Bacterial Proteins