Resolution in structured illumination microscopy: a probabilistic approach.
Structured illumination can be employed to extend the lateral resolution of wide-field fluorescence microscopy. Since a structured illumination microscopy image is reconstructed from a series of several acquired images, we develop a modified formulation of the imaging response of the microscope and a probabilistic analysis to assess the resolution performance. We use this model to compare the fluorescence imaging performance of structured illumination techniques to confocal microscopy. Specifically, we examine the trade-off between achievable lateral resolution and signal-to-noise ratio when photon shot noise is dominant. We conclude that for a given photon budget, structured illumination invariably achieves better lateral resolution than confocal microscopy.
Institute of Biophysics, Imaging and Optical Science, School of Electrical and Electronic Engineering, The University of Nottingham, University Park, Nottingham, NG7 2RD, UK. mike.somekh@nottingham.ac.uk
Michael G
MG
Somekh
Ken
K
Hsu
Mark C
MC
Pitter