Spike timing-dependent long-term depression requires presynaptic NMDA receptors.
NMDA receptors are necessary for both synaptic potentiation and depression, but the precise location of these receptors has not been established. By loading MK-801 into pre- or postsynaptic neurons during paired recordings of synaptically connected layer 4 and layer 2/3 neurons in mouse barrel cortex, we found that synaptic potentiation requires postsynaptic, but not presynaptic, NMDA receptors, whereas synaptic depression requires presynaptic, but not postsynaptic, NMDA receptors.
Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford OX1 3PT, UK. arodmor@upo.es
Antonio
A
Rodríguez-Moreno
Ole
O
Paulsen
D000200:Action Potentials / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D000818:Animals; D000831:Animals, Newborn; D016291:Dizocilpine Maleate / Q000494:pharmacology; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D036881:Long-Term Synaptic Depression / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D051379:Mice; D008810:Mice, Inbred C57BL; D018408:Patch-Clamp Techniques / Q000379:methods; D017729:Presynaptic Terminals / Q000187:drug effects / Q000502:physiology* / Q000528:radiation effects; D017966:Pyramidal Cells / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D016194:Receptors, N-Methyl-D-Aspartate / Q000502:physiology*; D013003:Somatosensory Cortex / Q000166:cytology; D014633:Valine / Q000031:analogs & derivatives / Q000494:pharmacology
D018691:Excitatory Amino Acid Antagonists; D016194:Receptors, N-Methyl-D-Aspartate; D016291:Dizocilpine Maleate; C056639:2-amino-5-phosphopentanoic acid; D014633:Valine