Detection of the dihydrofolate reductase-164L mutation in Plasmodium falciparum infections from Malawi by heteroduplex tracking assay.
Standard polymerase chain reaction methods often cannot detect drug-resistance mutations in Plasmodium falciparum infections if the mutation is present in < or = 20% of the parasites. A heteroduplex tracking assay was developed that can detect dihydrofolate reductase 164-L mutations in variants representing 1% of the parasites in an individual host. Using this assay, we confirmed the presence of the mutation in P. falciparum infections in Malawi.
Division of Infectious Diseases, University of North Carolina, School of Medicine, Chapel Hill, North Carolina 27599-7300, USA. jjuliano@med.unc.edu
Jonathan J
JJ
Juliano
Paul
P
Trottman
Victor
V
Mwapasa
Steven R
SR
Meshnick
D000818:Animals; D001483:Base Sequence; D015331:Cohort Studies; D017931:DNA Primers; D005260:Female; D006801:Humans; D016778:Malaria, Falciparum / Q000201:enzymology / Q000235:genetics*; D008295:Malawi; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes; D010963:Plasmodium falciparum / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000235:genetics; D012680:Sensitivity and Specificity; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D017931:DNA Primers; D009692:Nucleic Acid Heteroduplexes; D013762:Tetrahydrofolate Dehydrogenase