Caffeine induces Ca2+ release by reducing the threshold for luminal Ca2+ activation of the ryanodine receptor.
Caffeine has long been used as a pharmacological probe for studying RyR (ryanodine receptor)-mediated Ca(2+) release and cardiac arrhythmias. However, the precise mechanism by which caffeine activates RyRs is elusive. In the present study, we investigated the effects of caffeine on spontaneous Ca(2+) release and on the response of single RyR2 (cardiac RyR) channels to luminal or cytosolic Ca(2+). We found that HEK-293 cells (human embryonic kidney cells) expressing RyR2 displayed partial or 'quantal' Ca(2+) release in response to repetitive additions of submaximal concentrations of caffeine. This quantal Ca(2+) release was abolished by ryanodine. Monitoring of endoplasmic reticulum luminal Ca(2+) revealed that caffeine reduced the luminal Ca(2+) threshold at which spontaneous Ca(2+) release occurs. Interestingly, spontaneous Ca(2+) release in the form of Ca(2+) oscillations persisted in the presence of 10 mM caffeine, and was diminished by ryanodine, demonstrating that unlike ryanodine, caffeine, even at high concentrations, does not hold the channel open. At the single-channel level, caffeine markedly reduced the threshold for luminal Ca(2+) activation, but had little effect on the threshold for cytosolic Ca(2+) activation, indicating that the major action of caffeine is to reduce the luminal, but not the cytosolic, Ca(2+) activation threshold. Furthermore, as with caffeine, the clinically relevant, pro-arrhythmic methylxanthines aminophylline and theophylline potentiated luminal Ca(2+) activation of RyR2, and increased the propensity for spontaneous Ca(2+) release, mimicking the effects of disease-linked RyR2 mutations. Collectively, our results demonstrate that caffeine triggers Ca(2+) release by reducing the threshold for luminal Ca(2+) activation of RyR2, and suggest that disease-linked RyR2 mutations and RyR2-interacting pro-arrhythmic agents may share the same arrhythmogenic mechanism.
Department of Physiology and Biophysics, Libin Cardiovascular Institute of Alberta, 3330 Hospital Drive N.W., University of Calgary, Calgary, AB, Canada.
Huihui
H
Kong
Peter P
PP
Jones
Andrea
A
Koop
Lin
L
Zhang
Henry J
HJ
Duff
S R Wayne
SR
Chen
D000818:Animals; D002110:Caffeine / Q000378:metabolism / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects; D003600:Cytosol / Q000378:metabolism; D006801:Humans; D009154:Mutation; D032383:Myocytes, Cardiac / Q000276:immunology / Q000378:metabolism; D051381:Rats; D019837:Ryanodine Receptor Calcium Release Channel / Q000235:genetics / Q000378:metabolism*; D012519:Sarcoplasmic Reticulum / Q000378:metabolism; D014970:Xanthines / Q000494:pharmacology
D019837:Ryanodine Receptor Calcium Release Channel; D014970:Xanthines; C008514:methylxanthine; D002110:Caffeine; D002118:Calcium