Engineering of functional cartilage tissue using stem cells from synovial lining: a preliminary study.
Stem cells derived from synovial lining-synovial lining--derived stem cells or SDSCs--are a promising cell source for cartilage tissue engineering. We hypothesized that negatively selected SDSCs would form cartilage constructs and conventionally passaged SDSCs would be contaminated with macrophages, inhibiting SDSC-based chondrogenesis. We mixed SDSCs with fibrin gel and seeded the cells into polyglycolic acid scaffolds. After 3 days of incubation with a proliferative growth factor cocktail (containing transforming growth factor beta1 [TGF-beta1], insulin-like growth factor I [IGF-I], and basic fibroblast growth factor [FGF-2]), the cell-fibrin-polyglycolic acid constructs were transferred into rotating bioreactor systems and cultured with a chondrogenic growth factor cocktail (TGF-beta1/IGF-I) for up to 4 weeks. Tissue constructs based on negatively selected SDSCs had cartilaginous characteristics; were rich in glycosaminoglycans and collagen II; exhibited high expression of mRNA and protein for collagen II, aggrecan, and Sox 9; exhibited a negligible level of mRNA and protein for collagens I and X; and had an equilibrium modulus in the range of values measured for native human cartilage. Conventional passage yielded SDSCs with contaminating macrophages, which adversely affected the quality of tissue-engineered cartilage. We thus propose functional cartilage constructs could be engineered in vitro through the use of negatively isolated SDSCs.
Pei
Ming
M
Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, 3943 Health Sciences Center South, PO Box 9196, Morgantown, WV 26506-9196, USA. mpei@hsc.wvu.edu
He
Fan
F
Kish
Vincent L
VL
Vunjak-Novakovic
Gordana
G
D000818:Animals; D020219:Chondrogenesis / Q000187:drug effects; D006801:Humans; D007334:Insulin-Like Growth Factor I; D008264:Macrophages; D011100:Polyglycolic Acid / Q000737:chemistry; D033581:Stem Cell Transplantation; D013552:Swine; D013583:Synovial Membrane / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D016212:Transforming Growth Factor beta; D011100:Polyglycolic Acid; D007334:Insulin-Like Growth Factor I