[Effect of forsythia suspense extract on phagocytosis of peritoneal macrophages and NO production in vitro].
OBJECTIVE
To investigate the effect of forsythia suspensa (FS) extract on phagocytosis of peritoneal macrophages and NO production in vitro.


METHODS
The peritoneal macrophagess were isolated from BALB/c mice. After stained with CFDA-SE, the DH5alpha were co-cultured with peritoneal macrophagess for 3 h. The effect of FS extract on cyto-phagocytesis in vitro was analyzed by flow cytometry. The peritoneal macrophages were stimulated and activated by LPS in vitro. The effect of FS extract on NO production of the peritoneal macrophages in vitro was measured by NO assay kit.


RESULTS
FCM analysis showed that FS extract significantly promoted the phagocytosis of peritoneal macrophages at the final concentration of 40, 80, 160 mg/L, respectively (P<0.05). It also decreased the production of NO at different concentration induced by LPS (P<0.05).


CONCLUSIONS
FS extract can promote phagocytosis of peritoneal macrophages and inhibit NO production in vitro.
Institute of Tissue Transplantation and Immunology, Jinan University, Guangzhou, China.
Le-le
LL
Yin
Yao-ying
YY
Zeng
Hui-na
HN
Hou
D000818:Animals; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D031657:Forsythia / Q000737:chemistry*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D009569:Nitric Oxide