TCR signaling and environment affect vasoactive intestinal peptide receptor-1 (VPAC-1) expression in primary mouse CD4 T cells.
Strict regulation of T cell function is imperative to control adaptive immunity, and dysregulation of T cell activation can contribute to infectious and autoimmune diseases. Vasoactive intestinal peptide receptor-1 (VPAC-1), an anti-inflammatory G-protein coupled receptor, has been reported to be downregulated during T cell activation. However, the regulatory mechanisms controlling the expression of VPAC-1 in T cells are not well understood. Therefore, mouse splenic CD4 T cells were treated in complete media+/-anti-CD3 for 24h, total RNA isolated and VPAC-1 levels measured by qPCR. Surprisingly, we discovered that T cells incubated in complete media steadily upregulated VPAC-1 mRNA levels over time (24h). Importantly, CD4 T cells isolated from blood also showed elevated VPAC-1 expression compared to splenic T cells. Collectively, these data support that the vascular environment positively influences VPAC-1 mRNA expression that is negatively regulated by TCR signaling. This research was supported by a national service award (1KO1 DK064828) to G.D., the Center for Protease Research (2P20RR015566), and INBRE (P20 RR016741).
Vomhof-DeKrey
Emilie E
EE
Department of Chemistry and Molecular Biology, The Center for Protease Research, North Dakota State University, Room 320, IACC Building, 1320 Albrecht Boulevard, Fargo, ND 58105, USA.
Hermann
Rebecca J
RJ
Department of Chemistry and Molecular Biology, The Center for Protease Research, North Dakota State University, Room 320, IACC Building, 1320 Albrecht Boulevard, Fargo, ND 58105, USA.
Palmer
Megan F
MF
Department of Chemistry and Molecular Biology, The Center for Protease Research, North Dakota State University, Room 320, IACC Building, 1320 Albrecht Boulevard, Fargo, ND 58105, USA.
Benton
Keith D
KD
Department of Chemistry and Molecular Biology, The Center for Protease Research, North Dakota State University, Room 320, IACC Building, 1320 Albrecht Boulevard, Fargo, ND 58105, USA.
Sandy
Ashley R
AR
Department of Chemistry and Molecular Biology, The Center for Protease Research, North Dakota State University, Room 320, IACC Building, 1320 Albrecht Boulevard, Fargo, ND 58105, USA.
Dorsam
Sheri T
ST
Department of Chemistry and Molecular Biology, The Center for Protease Research, North Dakota State University, Room 320, IACC Building, 1320 Albrecht Boulevard, Fargo, ND 58105, USA.
Dorsam
Glenn Paul
GP
Department of Chemistry and Molecular Biology, The Center for Protease Research, North Dakota State University, Room 320, IACC Building, 1320 Albrecht Boulevard, Fargo, ND 58105, USA. Electronic address: glenn.dorsam@ndsu.edu.
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018929:Cell Culture Techniques; D003470:Culture Media / Q000494:pharmacology; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017260:Receptor-CD3 Complex, Antigen, T-Cell / Q000276:immunology / Q000502:physiology*; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I / Q000032:analysis / Q000235:genetics* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000502:physiology*
D000906:Antibodies; D003470:Culture Media; D012333:RNA, Messenger; D017260:Receptor-CD3 Complex, Antigen, T-Cell; D051238:Receptors, Vasoactive Intestinal Polypeptide, Type I