Serum CRP-like protein profile in common carp Cyprinus carpio challenged with Aeromonas hydrophila and Escherichia coli lipopolysaccharide.
The potential of C-reactive protein (CRP)-like proteins to be used as a biomarker of health status in cultured carp obtained from various European fish lines has been assessed. Varying CRP-like protein levels in the serum of carp were monitored using an indirect competitive enzyme-linked immunosorbent assay. CRP-like protein basal levels in normal fish varied between carp lines, ranging on average from 2.9+/-0.15 to 12.57+/-1.19 microg ml(-1). Serum levels of CRP-like protein in carp were observed to increase several fold in fish infected with the pathogen Aeromonas hydrophila. However, carp injected with Escherichia coli lipopolysaccharide (LPS) serotype 0111:B4 did not exhibit an increase in CRP-like proteins levels.
School of Life Sciences, Huxley Building, Keele University, Staffordshire ST5 5BG, UK.
Eugene M
EM
MacCarthy
Ian
I
Burns
Ilgiz
I
Irnazarow
Anthony
A
Polwart
Trevor J
TJ
Greenhough
Annette K
AK
Shrive
David
D
Hoole
D016980:Aeromonas hydrophila; D000595:Amino Acid Sequence; D000818:Animals; D002097:C-Reactive Protein / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002347:Carps / Q000097:blood*; D004926:Escherichia coli; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid
D008070:Lipopolysaccharides; D002097:C-Reactive Protein