Crystal structure of the cold-active aminopeptidase from Colwellia psychrerythraea, a close structural homologue of the human bifunctional leukotriene A4 hydrolase.
The crystal structure of a cold-active aminopeptidase (ColAP) from Colwellia psychrerythraea strain 34H has been determined, extending the number of crystal structures of the M1 metallopeptidase family to four among the 436 members currently identified. In agreement with their sequence similarity, the overall structure of ColAP displayed a high correspondence with leukotriene A4 hydrolase (LTA4H), a human bifunctional enzyme that converts leukotriene A4 (LTA4) in the potent chemoattractant leukotriene B4. Indeed, both enzymes are composed of three domains, an N-terminal saddle-like domain, a catalytic thermolysin-like domain, and a less conserved C-terminal alpha-helical flat spiral domain. Together, these domains form a deep cavity harboring the zinc binding site formed by residues included in the conserved HEXXHX(18)H motif. A detailed structural comparison of these enzymes revealed several plausible determinants of ColAP cold adaptation. The main differences involve specific amino acid substitutions, loop content and solvent exposure, complexity and distribution of ion pairs, and differential domain flexibilities. Such elements may act synergistically to allow conformational flexibility needed for an efficient catalysis in cold environments. Furthermore, the region of ColAP corresponding to the aminopeptidase active site of LTA4H is much more conserved than the suggested LTA4 substrate binding region. This observation supports the hypothesis that this region of the LTA4H active site has evolved in order to fit the lipidic substrate.
Bauvois
Cédric
C
Laboratoire de Microbiologie, Université Libre de Bruxelles, B-1070 Bruxelles, Belgium.
Jacquamet
Lilian
L
Huston
Adrienne L
AL
Borel
Franck
F
Feller
Georges
G
Ferrer
Jean-Luc
JL
D034241:Alteromonadaceae / Q000201:enzymology*; D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000626:Aminopeptidases / Q000737:chemistry*; D003080:Cold Temperature; D018360:Crystallography, X-Ray; D004851:Epoxide Hydrolases / Q000737:chemistry*; D006801:Humans; D008954:Models, Biological; D008969:Molecular Sequence Data; D011487:Protein Conformation; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry; D017386:Sequence Homology, Amino Acid; D013820:Thermolysin / Q000737:chemistry
D011994:Recombinant Proteins; D004851:Epoxide Hydrolases; D000626:Aminopeptidases; D013820:Thermolysin; C043134:leukotriene A4 hydrolase