Nano-composition of riboflavin-nafion functional film and its application in biosensing.
A novel nafion-riboflavin membrane was constructed and characterized by the scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV-visible spectroscopy and cyclic voltammetric techniques. The estimated average diameter of the designed nanoparticles was about 60 nm. The functional membrane showed a quasi-reversible electrochemical behaviour with a formal potential of -562 +/- 5 mV (vs Ag/AgCl) on the gold electrode. Some electrochemical parameters were estimated, indicating that the system has good and stable electron transfer properties. Moreover, horseradish peroxidase (HRP) was immobilized on the riboflavin-nafion functional membrane. The electrochemical behaviour of HRP was quasi-reversible with a formal potential of 80 +/- 5 mV (vs Ag/AgCl). The HRP in the film exhibited good catalytic activity towards the reduction of H2O2. It shows a linear dependence of its cathodic peak current on the concentration of H2O2, ranging from 10 to 300 (micro)M.
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
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Rezaei-Zarchi
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Saboury
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Javed
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Barzegar
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Ahmadian
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Bayandori-Moghaddam
D015374:Biosensing Techniques; D004563:Electrochemistry; D004566:Electrodes; D005465:Fluorocarbon Polymers / Q000737:chemistry*; D006046:Gold / Q000737:chemistry; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D049329:Nanostructures / Q000737:chemistry*; D012256:Riboflavin / Q000737:chemistry*
D005465:Fluorocarbon Polymers; D008567:Membranes, Artificial; C040402:perfluorosulfonic acid; D006046:Gold; D012256:Riboflavin