Low frequency of Epstein Barr virus association and high frequency of p53 overexpression in an Argentinean pediatric T-cell lymphoma series.
T-cell non-Hodgkin's lymphomas (NHLs) represent 10% to 15% of all diagnosed lymphomas in Western countries. Various geographic frequencies of T-cell NHL have been documented, in part reflecting increased exposure to pathogenic factors such as Epstein-Barr virus (EBV). Our aims were to assess EBV and p53 expression in Argentine pediatric T-cell lymphoma and to correlate them with patients' survival. Epstein-Barr encoded RNAs (EBERs) in situ hybridization and LMP1 and p53 immunohistochemical staining were performed on formalin-fixed paraffin-embedded lymph node biopsies from 25 pediatric T-lymphoma patients. In 17 of 25 samples good-quality DNA was obtained, and EBER polymerase chain reaction was assessed to confirm in situ hybridization and immunohistochemical results. Epstein-Barr virus expression was found in 8.0% of cases. p53-positive staining was distributed in 92% of pediatric cases. Kaplan-Meier survival analysis showed that neither EBV nor p53 expression was statistically significantly associated with event-free survival. Our data showed a low frequency of EBV association with pediatric T-cell lymphoma. It seems that p53 plays an important role in proliferation in our studied population, since it is overexpressed in 92% of T-cell lymphoma cases.
Chabay
Paola
P
Pathology Division, Ricardo Gutiérrez Children's Hospital, Buenos Aires, Argentina. paola_chabay@yahoo.com.ar
De Matteo
Elena
E
Lorenzetti
Mario
M
Barón
Anahí Vijnovich
AV
Valva
Pamela
P
Preciado
María Victoria
MV
D015703:Antigens, CD / Q000096:biosynthesis; D001118:Argentina; D014408:Biomarkers, Tumor / Q000032:analysis*; D002648:Child; D020031:Epstein-Barr Virus Infections / Q000150:complications / Q000453:epidemiology*; D005260:Female; D005434:Flow Cytometry; D004854:Herpesvirus 4, Human; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D053208:Kaplan-Meier Estimate; D015458:Leukemia, T-Cell / Q000378:metabolism* / Q000401:mortality / Q000821:virology*; D008297:Male; D016133:Polymerase Chain Reaction; D012367:RNA, Viral / Q000032:analysis; D016159:Tumor Suppressor Protein p53 / Q000096:biosynthesis*
D015703:Antigens, CD; D014408:Biomarkers, Tumor; D012367:RNA, Viral; D016159:Tumor Suppressor Protein p53