LC-MS for protein characterization: current capabilities and future trends.
With advances in ionization methods and instrumentation, liquid chromatography (LC)/mass spectrometry (MS) has become a powerful technology for protein characterization. This review article will describe the general approaches on LC-MS analysis in protein characterization, including bottom-up and top-down strategies. Discussions will be given on characterization of recombinant proteins, and post-translational and protein modifications such as disulfide bonds, glycosylation and phosphorylation using LC-MS. New research directions in this area will also be presented to illustrate future prospects of LC-MS in protein characterization, including application to proteomics.
Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033, USA. guodong.chen@spcorp.com
Guodong
G
Chen
Birendra N
BN
Pramanik
D002853:Chromatography, Liquid / Q000379:methods*; D006031:Glycosylation; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D010766:Phosphorylation; D011506:Proteins / Q000737:chemistry*
D011506:Proteins