Proteomic analysis in the lichen Physcia adscendens exposed to cadmium stress.
This work was undertaken to explore the potential of proteomics to dissect parallel and consecutive events of cadmium stress response in the lichen Physcia adscendens (Fr.) H. Olivier. Thalli were exposed to 0 (control) and 36 microM Cd for 6, 18, 24 and 48 h. Two-dimensional electrophoresis and mass spectrometry analyses showed an 80-85% spot identity between 6 and 18 h vs. 24 and 48 h of Cd exposure. Putative heat-shock proteins and glutathione S-transferase generally increased their expression all over the Cd treatments. By contrast, ABC transporters were underexpressed after 6-18 h, but in some cases induced after 24-48 h of Cd exposure. The cytochrome P450 appeared to have a variable expression pattern over time. Overall these data suggest that a considerable importance in the response of P. adscendens thalli to Cd stress can be assumed by differential expression of various protein families.
Rustichelli
C
C
Department of Environmental Sciences, Division of Genetics and Environmental Biotechnology, University of Parma, viale G.P. Usberti, 11/A, 43100 Parma, Italy.
Visioli
G
G
Kostecka
D
D
Vurro
E
E
di Toppi
L Sanità
LS
Marmiroli
N
N
D018528:ATP-Binding Cassette Transporters / Q000032:analysis / Q000378:metabolism; D000393:Air Pollutants / Q000633:toxicity*; D002104:Cadmium / Q000633:toxicity*; D002734:Chlorophyll / Q000032:analysis; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional; D005982:Glutathione Transferase / Q000032:analysis; D018840:HSP70 Heat-Shock Proteins / Q000032:analysis; D008011:Lichens / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D018528:ATP-Binding Cassette Transporters; D000393:Air Pollutants; D018840:HSP70 Heat-Shock Proteins; D002104:Cadmium; D002734:Chlorophyll; D003577:Cytochrome P-450 Enzyme System; D005982:Glutathione Transferase