Properties of deproteinized bone for reparation of big segmental defect in long bone.
OBJECTIVE
To explore suitable scaffold material for big segmental long bone defect by studying the properties of the prepared deproteinized bone.


METHODS
Cancellated bone were made as 30 mm x mm x 3 mm bone blocks from inferior extremity of pig femur along bone trabecula. The deproteinized bone was prepared with an improved method. Their morphological features, components, cell compatibility, mechanical and immunological properties were investigated respectively.


RESULTS
Deproteinized bone maintained natural reticular pore system. The main organic material is collagen I and inorganic composition is hydroxyapatite. It has good mechanical properties, cell adhesion rate and histocompatibility.


CONCLUSIONS
This deproteinized bone can be applicable as scaffold for reparation of big segmental defect in long bone.
Jian
Yue-kui
YK
Department of Orthopedics, Guizhou Provincial People's Hospital, Gui Yang 550002, China. jianyuekui@sina.com
Tian
Xiao-bin
XB
Li
Bo
B
Qiu
Bing
B
Zhou
Zuo-jia
ZJ
Yang
Zheng
Z
Li
Qi-hong
QH
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology; D016025:Bone Transplantation / Q000379:methods*; D003094:Collagen; D006882:Hydroxyapatites; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
D006882:Hydroxyapatites; D003094:Collagen