[Computer simulation of complex formation between plastocyanine and cytochrome f in thylakoid lumen].
The diffusion of the protein plastocyanine and complex formation between plastocyanine and cytochrome f (a subunit of a cytochrome b6/f complex) in the chloroplast thylakoid lumen has been studied. A 3D computer simulation model of diffusion and binding of plastocyanine and cytochrome f has been constructed, which considers their electrostatic interaction. Based on the experimental data, the parameters of the model for complex formation between plastocyanine and cytochrome f in solution have been estimated. The dependence of the rate of plastocyanine-cytochrome f reaction on the size of the luminal space has been studied. It was shown that the contraction of the luminal space leads to a decrease in the reaction rate, which is in agreement with the experimental data on the inhibition of the reaction under hyperosmotic stress.
Kovalenko
I B
IB
Abaturova
A M
AM
Gromov
P A
PA
Ustinin
D M
DM
Riznichenko
G Iu
GIu
Grachev
E A
EA
Rubin
A B
AB
D003198:Computer Simulation; D045348:Cytochromes f / Q000737:chemistry*; D004058:Diffusion; D010970:Plastocyanin / Q000737:chemistry*; D011485:Protein Binding; D055672:Static Electricity; D020524:Thylakoids / Q000737:chemistry*
D010970:Plastocyanin; D045348:Cytochromes f