Gene expression profiling of diffuse large B-cell lymphoma supervised by CD21 expression.
This study investigated the gene expression profiles of 40 cases of diffuse large B-cell lymphoma (DLBCL) according to CD21 expression, a favourable prognostic factor in DLBCL. Signature genes were analysed by Gene Ontology Tree Machine, and genes concerned with the immune system and related categories were significantly upregulated in CD21- DLBCLs. Of 40 DLBCLs, four were germinal centre B cell-like (GCB) and 36 non-GCB. Of the 36 non-GCB DLBCLs, 14 CD21+ DLBCLs showed significantly better overall survival than the 22 CD21- DLBCLs (P = 0.036). Hierarchical cluster analysis of signature genes related to CD21 was applied to previously published data sets, resulting in two groups for each data set, CD21+ type DLBCLs and CD21- type DLBCLs. Survival of CD21+ type DLBCLs was significantly better than that of CD21- type (P = 0.006 and P = 0.004, respectively). In both data sets, CD21+ type DLBCLs predominantly included GCB DLBCLs compared with CD21- type. The top classifier gene of CD21 expression was IGHM, and the five of nine Gene Ontology categories significant in CD21- DLBCLs included IGHM. Immunohistochemical analysis of 216 DLBCLs confirmed that overall survival of surface (s) IgM+ DLBCLs was significantly poorer than that of sIgM- DLBCLs (P = 0.013).
Miyazaki
Kana
K
Department of Haematology and Oncology, Mie University Graduate School of Medicine, Tsu, Japan.
Yamaguchi
Motoko
M
Suguro
Miyuki
M
Choi
Woonyoung
W
Ji
Yuan
Y
Xiao
Lianchun
L
Zhang
Wei
W
Ogawa
Shoko
S
Katayama
Naoyuki
N
Shiku
Hiroshi
H
Kobayashi
Tohru
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000378:metabolism; D005260:Female; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D016403:Lymphoma, Large B-Cell, Diffuse / Q000235:genetics* / Q000276:immunology; D008297:Male; D046228:Microarray Analysis; D008875:Middle Aged; D017464:Receptors, Complement 3d / Q000378:metabolism*
D014408:Biomarkers, Tumor; D017464:Receptors, Complement 3d