The epigenetics of adult (somatic) stem cells.
While genetic studies have provided a wealth of information about health and disease, there is a growing awareness that individual characteristics are also determined by factors other than genetic sequences. These "epigenetic" changes broadly encompass the influence of the environment on gene regulation and expression and in a more narrow sense, describe the mechanisms controlling DNA methylation, histone modification and genetic imprinting. In this review, we focus on the epigenetic mechanisms that regulate adult (somatic) stem cell differentiation, beginning with the metabolic pathways and factors regulating chromatin structure and DNA methylation and the molecular biological tools that are currently available to study these processes. The role of these epigenetic mechanisms in manipulating adult stem cells is followed by a discussion of the challenges and opportunities facing this emerging field.
Epigenetics and Nuclear Reprogramming Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA 70808, USA. ertkj@pbrc.edu
Kenneth J
KJ
Eilertsen
Z
Z
Floyd
Jeffrey M
JM
Gimble
D000818:Animals; D002454:Cell Differentiation; D019070:Cell Lineage; D002843:Chromatin / Q000378:metabolism; D019175:DNA Methylation; D004622:Embryo, Mammalian / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D044127:Epigenesis, Genetic; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D013234:Stem Cells / Q000378:metabolism*
D002843:Chromatin