PCR analysis of the Tri13 gene to determine the genetic potential of Fusarium graminearum isolates from Iran to produce nivalenol and deoxynivalenol.
Fusarium graminearum trichothecene producing isolates can be broadly divided into two chemotypes based on the production of the 8- ketotrichothecenes deoxynivalenol (DON) and nivalenol (NIV). Functional Tri13 gene required for the production of NIV and 4- acetyl NIV, whereas in the isolates producing DON and its acetylated derivates, this gene is nonfunctional. In this study, a total of 57 isolates from different fields of Mazandaran province, Iran were identified as F. graminearum using classical methods and species specific primers. In order to assess the potential of isolates to produce NIV or DON, we used PCR to determine whether isolates carried a functional or nonfunctional Tri13 gene. Out of the 57 tested F. graminearum isolates with Tri13 PCR assays, 46 yielded an amplicon similar to the size predicted for nivalenol production, while 11 yielded an amplicon similar to the size predicted for deoxynivalenol production. From regions where more than one F. graminearum isolate was obtained, isolates were not exclusively of a single chemotype. It seems that genetic diversity among the isolates has relation with geographical region and wheat cultivar. The assay can provide information about the distribution of Tri13 haplotype that can be used in tracing of trichothecene contaminated samples.
Haratian
Mahboobeh
M
Department of Plant Protection, College of Agriculture, Isfahan University of Technology, Isfahan, Iran.
Sharifnabi
Bahram
B
Alizadeh
Azizollah
A
Safaie
Naser
N
D000383:Agriculture / Q000379:methods; D005670:Fusarium / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D005800:Genes, Fungal / Q000235:genetics*; D007492:Iran; D010935:Plant Diseases / Q000382:microbiology; D016133:Polymerase Chain Reaction; D014255:Trichothecenes / Q000378:metabolism; D014908:Triticum / Q000382:microbiology
D014255:Trichothecenes; C038405:nivalenol; C007262:deoxynivalenol