Application of fluorescence lifetime imaging of enhanced green fluorescent protein to intracellular pH measurements.
We have shown that the intracellular pH of a single HeLa cell expressing the enhanced green fluorescent protein (EGFP) can be imaged using the fluorescence lifetime of EGFP, which can be interpreted in terms of the pH-dependent ionic equilibrium of the p-hydroxybenzylidene-imidazolidinone structure of the chromophore of EGFP.
Nakabayashi
Takakazu
T
Research Institute for Electronic Science, Hokkaido University, Sapporo, 060-0812, Japan.
Wang
Hui-Ping
HP
Kinjo
Masataka
M
Ohta
Nobuhiro
N
D005453:Fluorescence; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D013997:Time Factors
D049452:Green Fluorescent Proteins