The effects of anesthetic agent and carrier gas on blood glucose and tissue uptake in mice undergoing dynamic FDG-PET imaging: sevoflurane and isoflurane compared in air and in oxygen.
OBJECTIVE
We sought to identify an anesthetic regime that, unlike isoflurane in air, would maintain glucose homeostasis in mice undergoing Positron emission tomography (PET) imaging with 2-deoxy-2-[18F]fluoro-D: -glucose (FDG).


METHODS
FDG uptake was also measured in normal and tumor tissues. Athymic and Balb/c nude mice were studied. Blood glucose levels were measured before and after 30 min of FDG PET imaging under isoflurane or sevoflurane carried in air or oxygen. FDG uptake was quantified as a percentage of the injected dose and using Patlak analysis yielding Ki values.


RESULTS
Blood glucose levels were more stable under sevoflurane than under isoflurane, especially in the athymic nude mice. Under isoflurane, FDG uptake into myocardium was higher than under sevoflurane and was strongly correlated with the intrascan change in blood glucose.


CONCLUSIONS
Sevoflurane should be preferred for physiologic imaging in mice, minimizing changes in glucose and, for FDG PET, reducing signal spillover from the myocardium.
Department of Biomedical Imaging, Genentech, 1 DNA Way MS 228, South San Francisco, CA 94080, USA.
Judith E
JE
Flores
Leanne M
LM
McFarland
Alexander
A
Vanderbilt
Annie K
AK
Ogasawara
Simon-Peter
SP
Williams
D000388:Air; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis; D045744:Cell Line, Tumor; D015331:Cohort Studies; D003110:Colonic Neoplasms / Q000473:pathology; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D005740:Gases / Q000494:pharmacology*; D045325:HCT116 Cells; D006801:Humans; D007530:Isoflurane / Q000494:pharmacology; D008297:Male; D008738:Methyl Ethers / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D010100:Oxygen / Q000494:pharmacology; D011471:Prostatic Neoplasms / Q000473:pathology; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D000077149:Sevoflurane; D014018:Tissue Distribution; D014055:Tomography, Emission-Computed; D023041:Xenograft Model Antitumor Assays
D018685:Anesthetics, Inhalation; D001786:Blood Glucose; D005740:Gases; D008738:Methyl Ethers; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D000077149:Sevoflurane; D007530:Isoflurane; D010100:Oxygen