Novel high-throughput profiling of human transcription factors and its use for systematic pathway mapping.
Transcription factors (TFs) are crucial components of regulatory networks that control gene transcription. Current TF assays are limited to the analysis of a single TF or require TF-specific antibodies. Here we report the Single Primer Amplification assisted Oligonucleotide Array-based Transcription Factor Assay (SPA-OATFA) which can directly analyze the binding activities of 240 human TFs simultaneously. Examining early events during serum-stimulation of HeLa cells as a model, we demonstrated the utility of SPA-OATFA combined with whole genome gene expression to systematically map the temporal activation of signaling pathways. Both TFs known to function in this stimulation response such as EGR1 and AP1 and new TFs such as HSF1 were identified. This information, combined with mRNA profiling, provided novel insights into the activities of regulatory pathways, and illustrates the potential of SPA-OATFA in detailed systems biology analysis of cell responses.
Medical Systems Biology Research Center, Tsinghua University, Beijing 100084, China.
Ji-Ying
JY
Qiao
Wei
W
Shao
Hua-Jiang
HJ
Wei
Yi-Min
YM
Sun
Yong-Chao
YC
Zhao
Wan-Li
WL
Xing
Liang
L
Zhang
Keith
K
Mitchelson
Jing
J
Cheng
D002467:Cell Nucleus / Q000378:metabolism; D020869:Gene Expression Profiling; D006367:HeLa Cells; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D011485:Protein Binding; D012333:RNA, Messenger / Q000378:metabolism; D015398:Signal Transduction; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D014157:Transcription Factors