Determining the concentration and the absorptivity factor at 260 nm in sodium dodecyl sulfate of the adenovirus reference material using analytical ultracentrifugation.
A concentration of 6.4 +/- 0.2 (at 1 SD) x 10(11) virus particles (vp) per milliliter was determined for the adenovirus reference material (ARM) by averaging analytical ultracentrifugation concentration data obtained with refractometric detection with the completely independent concentration reported by the Adenoviral Reference Material Working Group (ARMWG). Using this concentration, the ARM absorptivity factor (in sodium dodecyl sulfate [SDS] at 260 nm) of 1.2+/-0.1 (at 1 SD) x 10(12) vp/ml per OD(260 nm) per centimeter was obtained.
Department of Analytical Development, Biogen Idec, Cambridge, MA 02142, USA. steven.berkowitz@biogenidec.com
Steven A
SA
Berkowitz
D000042:Absorption; D000256:Adenoviridae; D012015:Reference Standards; D012031:Refractometry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D014461:Ultracentrifugation / Q000592:standards*
D012967:Sodium Dodecyl Sulfate