Subproteomic analysis of the mitochondrial proteins in rats 24 h after partial hepatectomy.
A 70% partial hepatectomy (70%PHx) induces cell proliferation until the original mass of the liver is restored. Mitochondria are involved directly in the process of liver regeneration (LR); however, their role in the early phase of LR is not clear. In an attempt to identify mitochondrial proteins that are correlated with the early phase of LR, we obtained a mitochondrial fraction via Nycodenz(R) density gradient centrifugation and subcellular proteomic analysis was performed. The mitochondrial proteins were separated by two-dimensional gel electrophoresis and identified by mass spectrometry. Compared to the sham-operation control group, 3 proteins were up-regulated and 22 proteins were down-regulated at 24 h after 70%PHx. We identified 22 differentially expressed proteins that were associated with carbohydrate metabolism, lipid metabolism, the respiratory chain and oxidation-phosphorylation, biotransformation and other metabolic pathways. Prohibitin, a proliferation-regulating protein that was down-regulated at 24 h after PHx, was analyzed by applying RNAi (PHBi) with BRL-3A. This demonstrated a decreased mitochondrial membrane potential, implying a potential role in maintaining mitochondrial integrity. These results indicated that hepatic mitochondrial adaptations to LR after 70%PHx affect various cellular metabolic pathways, which advances our knowledge of the role of mitochondria in the early phase of LR.
Sun
Qingju
Q
Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai 200433, China.
Miao
Mingyong
M
Jia
Xuan
X
Guo
Wei
W
Wang
Lianghua
L
Yao
Zhenzhen
Z
Liu
Chunli
C
Jiao
Binghua
B
D000818:Animals; D015153:Blotting, Western; D002460:Cell Line; D019295:Computational Biology; D015180:Electrophoresis, Gel, Two-Dimensional; D006498:Hepatectomy; D008297:Male; D053078:Membrane Potential, Mitochondrial; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D040901:Proteomics; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013997:Time Factors
D024101:Mitochondrial Proteins; D034741:RNA, Small Interfering