[The analysis of the content of bile acids in ox-bile powder and goat gall powder of Mongolian medicine].
The present study was to detect and compare the content of bile acids in ox bile powder and goat gall powder of Mongolia medicine by UV. Cholic acid with sulphuric acid were heated and dehydrated, and they produced conjugated double bond. The conjugated bond showed the same absorption peak in the ultraviolet range. The method of ultraviolet spectrophotometry can be used to detect and compare the content of bile acids in ox bile powder and goat gall powder. The result showed that the linear range was 0.003 3-0.016 7 mg x mL(-1) (r = 0.999 7). The average recovery (n = 5) of standard addition method of ox bile powder and goat gall powder was 98.48% (RSD = 1.79%) and 96.46% (RSD = 2.50%) respectively. The result of determination of five different samples showed that the content of bile acids in ox bile powder and goat gall powder was 40.85%-43.03% and 30.88%-32.64% respectively. The RSD of the analysis of ox bile powder and goat ball powder was 2.40% and 2.92% respectively, the RSD of stationary test of ox bile powder and goat ball powder in eight hours was 0.55% and 0.59% respectively, and the RSD of reproducibility of the analysis of ox bile powder and goat ball powder was 2.11% and 2.68% respectively. The method was simple, accurate, fast and easy to generalize and apply in many fields. It can be used to control the quality of ox bile powder and goat gall powder.
The School of Pharmaceutical Sciences, Inner Mongolia Medical College, Huhhot 010059, China. hhhtnmyxyjih5511@sina.com
Ai-Hua
AH
Ju
Ya-Shu
YS
Xun
Xiu-Lan
XL
Su
Wan-Fu
WF
Bai
Hui-Wen
HW
Zhang
Xiu-Juan
XJ
Zhou
D000818:Animals; D001646:Bile / Q000737:chemistry*; D001647:Bile Acids and Salts / Q000032:analysis*; D002417:Cattle; D005704:Gallbladder / Q000737:chemistry*; D056785:Medicine, Mongolian Traditional; D011208:Powders / Q000032:analysis; D015203:Reproducibility of Results; D013053:Spectrophotometry
D001647:Bile Acids and Salts; D011208:Powders