Continuous synthesis of glyceryl ferulate using immobilized Candida antarctica lipase.
Glyceryl ferulate was synthesized through condensation of ferulic acid and glycerol at 50 degrees C in glycerols with different water contents using an immobilized lipase from Candida antarctica in a batch reactor, and condensation in the glycerol with a 7.5% (w/w) water content was shown to be the favorite. The solubility of ferulic acid was higher at higher temperature in glycerol with a lower water content. The viscosity was lower at higher temperature for the glycerol with a higher water content. The condensation was carried out using a batch reactor at a temperature from 50 degrees C to 90 degrees C. These observations indicated that the condensation at 80 degrees C in the glycerol with a 7.5% (w/w) water content was the most adequate for continuously synthesizing glyceryl ferulate. A reactor system was constructed for the continuous synthesis and was steadily operated to realize a productivity of 430 kg/(m(3)-reactor(day) without any decrease in the conversion for at least 6 days.
Matsuo
Takemasa
T
Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.
Kobayashi
Takashi
T
Kimura
Yukitaka
Y
Hosoda
Asao
A
Taniguchi
Hisaji
H
Adachi
Shuji
S
D019149:Bioreactors; D002175:Candida / Q000201:enzymology*; D004800:Enzymes, Immobilized / Q000378:metabolism*; D005990:Glycerol; D008049:Lipase / Q000378:metabolism*; D050178:Monoglycerides / Q000378:metabolism*; D012995:Solubility; D013696:Temperature; D014783:Viscosity; D014867:Water / Q000378:metabolism
D004800:Enzymes, Immobilized; D050178:Monoglycerides; C531553:glyceryl ferulate; D014867:Water; D008049:Lipase; D005990:Glycerol