[Effects of root zone hypoxia on respiratory metabolism of cucumber seedlings roots].
With the seedlings of Lübachun No. 4, a hypoxia-resistant cultivar, and Zhongnong No. 8, a hypoxia-sensitive cultivar, as test materials, and by the method of solution culture, this paper studied the effects of root zone hypoxia on their roots' respiratory metabolism. The results showed that root zone hypoxia inhibited the tricarboxylic acid (TCA) cycle significantly, But accelerated the anaerobic respiration of cucumber roots. Under root zone hypoxia stress, the decrement of succinic dehydrogenase (SDH) and isocitric dehydrogenase (IDH) activities and the increment of lactate dehydrogenase (LDH) activity and lactate and pyruvate contents were lesser in Lübachun No. 4 than in Zhongnong No. 8 seedlings roots, but conversely, the increment of pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH) activities and alcohol and alanine contents in Lübachun No. 4 seedlings roots were higher than those in Zhongnong No. 8 seedlings roots. On the 8th day of hypoxia stress, the ADH activity and alcohol and alanine contents increased by 409.30%, 112.13% and 30.64% in Lübachun No. 4 roots and by 110.42%, 31.84% and 4.78% in Zhongnong No. 8 roots, respectively, compared with the control. No significant differences in the alanine aminotransferase (AlaAT) activity and acetaldehyde content were observed between the two cultivars. It was concluded that the acceleration of alcohol fermentation and the accumulation of alanine were in favor of the enhancement of root zone hypoxia tolerance of cucumber roots.
College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China. kyy_nau@sohu.com
Yun-Yan
YY
Kang
Shi-Rong
SR
Guo
Jiu-Ju
JJ
Duan
D015687:Cell Hypoxia; D019069:Cell Respiration; D018553:Cucumis sativus / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology*; D018517:Plant Roots / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D036226:Seedlings / Q000166:cytology / Q000254:growth & development / Q000378:metabolism*; D012987:Soil / Q000032:analysis
D012987:Soil