Cre-mediated recombination in mouse Clara cells.
Clara cells are nonciliated secretory cells lining the respiratory epithelium and are easily identified by the expression of Clara cell secretory protein (CCSP). To investigate molecular mechanism(s) regulating Clara cell function in the lungs, Cre recombinase was inserted into exon 1 of the CCSP, generating two novel mouse models, CCSP(Cre-Neo) and CCSP(Cre). These two models differ only by the inclusion of the neomycin resistance gene. These mice were bred to the R26R reporter mouse to investigate the tissue and cell specificity of Cre-mediated recombination. The efficiency of Cre recombination in the CCSP(Cre) mouse model was higher than in the CCSP(Cre-Neo) mouse model. Recombination was detected at D 4.5 in CCSP(Cre-Neo)/R26R mice and at D 0.5 in CCSP(Cre)/R26R mice. The CCSP(Cre-Neo) and CCSP(Cre) mouse models provide valuable tools for the ablation of genes in the postnatal mouse Clara cells.
Li
HuaiGuang
H
Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
Cho
Sung Nam
SN
Evans
Christopher M
CM
Dickey
Burton F
BF
Jeong
Jae-Wook
JW
DeMayo
Francesco J
FJ
D000818:Animals; D003433:Crosses, Genetic; D004847:Epithelial Cells / Q000378:metabolism; D005091:Exons; D018390:Gene Targeting / Q000379:methods; D017930:Genes, Reporter; D005818:Genetic Engineering; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D051379:Mice; D008822:Mice, Transgenic; D008957:Models, Genetic; D011995:Recombination, Genetic; D020545:Respiratory Mucosa / Q000166:cytology* / Q000378:metabolism*; D014598:Uteroglobin / Q000235:genetics* / Q000378:metabolism
C556390:Scgb1a1 protein, mouse; D014598:Uteroglobin; C045073:Cre recombinase; D019426:Integrases