Neuritogenic activity-guided isolation of a free base form manzamine A from a marine sponge, Acanthostrongylophora aff. ingens (Thiele, 1899).
Two manzamine-class alkaloids, manzamine A (1) and 8-hydroxymanzamine (2) were isolated from a Japanese marine sponge Acanthostrongylophora aff. ingens, together with three known alkaloids manzamine E (3), manzamine F (4), and manzamine X (5). The spectral features of 1 and 2 were different from the reported data. Detailed structure analysis using 2D NMR revealed the structure of 1 and 2 as a free base form of hydrochloric salt. These manzamine-class alkaloids showed neuritogenic activity against Neuro 2a cells.
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan.
Bo
B
Zhang
Ryuichi
R
Higuchi
Tomofumi
T
Miyamoto
Rob W M
RW
Van  Soest
D000818:Animals; D002227:Carbazoles / Q000302:isolation & purification* / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D018922:HL-60 Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D008968:Molecular Conformation; D016501:Neurites / Q000187:drug effects*; D011161:Porifera / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D002227:Carbazoles; C078290:manzamine A