Coating of vesicles with hydrophilic reactive polymers.
Vesicles bearing either cationic (amino) groups or zwitterionic (amino acid) groups on the surface were coated with a reactive multivalent hydrophilic N-(2-hydroxypropyl)methacrylamide polymer (PHPMA) and its positively charged analogue (3 mol % quaternary ammonium groups), both having reactive thiazolidine-2-thione (TT) groups randomly distributed along the polymer chain. The vesicles were dispersed in water at a concentration of 1 mg/mL. The effect of surface charges of model vesicles on the surface coating efficiency was evaluated. The changes in the weight-average molecular weight, in the hydrodynamic size, and in the zeta-potential of model vesicles were tested using light scattering methods. The most effective coating of vesicles was observed for the zwitterionic vesicles coated with the positively charged hydrophilic PHPMA-TT copolymer at a concentration of reactive polymer cp = 2 mg/mL. The coating efficiency was more than 1 order of magnitude higher than that obtained for positively charged vesicles coated by the uncharged hydrophilic polymer at the same cp.
Konák
Cestmír
C
Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.
Subr
Vladimír
V
Kostka
Libor
L
Stepánek
Petr
P
Ulbrich
Karel
K
Schlaad
Helmut
H
D002412:Cations / Q000737:chemistry; D015394:Molecular Structure; D008970:Molecular Weight; D011109:Polymethacrylic Acids / Q000737:chemistry*; D053778:Thiazolidines / Q000737:chemistry; D014867:Water / Q000737:chemistry*
D002412:Cations; D011109:Polymethacrylic Acids; D053778:Thiazolidines; D014867:Water; C011533:Duxon; C005104:2-mercaptothiazoline