Microbial oligosaccharides differentially induce volatiles and signalling components in Medicago truncatula.
Plants perceive biotic stimuli by recognising a multitude of different signalling compounds originating from the interacting organisms. Some of these substances represent pathogen-associated molecular patterns, which act as general elicitors of defence reactions. But also beneficial microorganisms like rhizobia take advantage of compounds structurally related to certain elicitors, i.e. Nod-factors, to communicate their presence to the host plant. In a bioassay-based study we aimed to determine to what extent distinct oligosaccharidic signals are able to elicit overlapping responses, including the emission of volatile organic compounds which is mainly considered a typical mode of inducible indirect defence against herbivores. The model legume Medicago truncatula Gaertn. was challenged with pathogen elicitors (beta-(1,3)-beta-(1,6)-glucans and N,N',N'',N'''-tetraacetylchitotetraose) and two Nod-factors, with one of them being able to induce a nodulation response in M. truncatula. Single oligosaccharidic elicitors caused the emission of volatile organic compounds, mainly sesquiterpenoids. The volatile blends detected were quite characteristic for the applied compounds, which could be pinpointed by multivariate statistical methods. As potential mediators of this response, the levels of jasmonic acid and salicylic acid were determined. Strikingly, neither of these phytohormones exhibited changing levels correlating with enhanced volatile emission. All stimuli tested caused an overproduction of reactive oxygen species, whereas nitric oxide accumulation was only effected by elicitors that were equally able to induce volatile emission. Thus, all signalling compounds tested elicited distinct reaction patterns. However, similarities between defence reactions induced by herbivory and pathogen-derived elicitors could be ascertained; but also Nod-factors were able to trigger defence-related reactions.
Leitner
Margit
M
Max Planck Institute for Chemical Ecology, Department Bioorganic Chemistry, Jena, Germany.
Kaiser
Roland
R
Rasmussen
Martin O
MO
Driguez
Hugues
H
Boland
Wilhelm
W
Mithöfer
Axel
A
D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D046913:Medicago truncatula / Q000187:drug effects* / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism; D009844:Oligosaccharides / Q000737:chemistry* / Q000494:pharmacology*; D010834:Phytochrome / Q000378:metabolism; D010838:Phytophthora / Q000737:chemistry; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects*; D014835:Volatilization
D009844:Oligosaccharides; D017382:Reactive Oxygen Species; D010834:Phytochrome; D009569:Nitric Oxide