Recombinant Newcastle disease virus expressing human interleukin-2 serves as a potential candidate for tumor therapy.
A recombinant Newcastle disease virus (NDV) containing human interleukin-2 (IL-2) gene was generated by applying reverse genetics technique and further evaluated for its suitability to express and deliver IL-2 for cancer therapy. We have further analyzed the ability of rNDV/IL2 to express IL-2 in several human tumor cell lines, including the human breast carcinoma cell line MCF-7, the human colon-adenocarcinoma cell line HT29, and human Jurkat cell line. IL-2 expressed by tumor cells infected with rNDV/IL-2 was stable up till 16 days, at body temperature, and with biological activity. Expression kinetics indicated that the expression level of IL-2 was already high at 24 h after infection and reached the highest level at 48 h after infection. As NDV was proposed as a very promising oncolytic agent in a new age of therapeutic viruses, our data strongly support the application of recombinant NDV/IL-2 virus as an anti-tumor agent.
Department of Food Science and Nutritional Engineering, China Agricultural University, 100083 Beijing, PR China. hengzhao2000@gmail.com
Heng
H
Zhao
Markus
M
Janke
Philippe
P
Fournier
Volker
V
Schirrmacher
D045744:Cell Line, Tumor; D006801:Humans; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D009369:Neoplasms / Q000276:immunology* / Q000821:virology*; D009522:Newcastle disease virus / Q000235:genetics / Q000254:growth & development*; D050504:Oncolytic Viruses / Q000235:genetics / Q000254:growth & development*
C508594:IL2 protein, human; D007376:Interleukin-2