A DNA transporter based on mesoporous silica nanospheres mediated with polycation poly(allylamine hydrochloride) coating on mesopore surface.
Mesoporous silica nanospheres (MPNSs) were synthesized by the sol-gel method and were investigated by X-ray diffraction, nitrogen sorption, and transmission electron microscopy. The analyses showed that the MPNSs were highly dispersed, with a diameter range of 50-250 nm. Further, the polycation poly(allylamine hydrochloride) was coated on silica MPNSs to form a positively charged surface and to facilitate the loading of DNA molecules. The composite was successfully used as a intercellular carrier of DNA molecules, and this was confirmed by agarose gel electrophoresis and confocal laser scanning microscopy. Evidently, this composite has potential to be used as a carrier for a DNA delivery system.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Science, Shanghai, China.
Fei
F
Qin
Yuchuan
Y
Zhou
Jianlin
J
Shi
Yonglian
Y
Zhang
D000818:Animals; D002412:Cations; D002460:Cell Line; D004247:DNA / Q000737:chemistry; D004587:Electrophoresis, Agar Gel / Q000379:methods; D051379:Mice; D018613:Microscopy, Confocal / Q000379:methods; D046529:Microscopy, Electron, Transmission; D054118:Nanospheres / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties; D014961:X-Ray Diffraction
D002412:Cations; D011073:Polyamines; C063994:polyallylamine; D012822:Silicon Dioxide; D004247:DNA; D009584:Nitrogen