Anti-tumor effects of water-soluble propolis on a mouse sarcoma cell line in vivo and in vitro.
The honeybee product propolis and its extracts are known to have biological effects such as antibiotic, anti-viral, anti-inflammatory and anti-tumor activities. This study was designed to investigate whether water-soluble propolis (WSP) inhibits tumor growth. The tumor cell line used was mouse sarcoma 180 (S-180), and its growth was determined in vitro and in vivo with exposure to different concentrations of WSP. The effects of WSP on tumor cells in vitro were evaluated by measuring the intracellular uptake of 3H-thymidine. 3H-thymidine uptake was inhibited in accordance with the concentration of WSP. The minimum concentration of WSP necessary for 3H-thymidine uptake inhibition was 1.0 microg/ml and uptake was suppressed to 88% of the level in non-treated cells at this concentration. In an experiment using tumor-bearing mice, oral administration of WSP was begun 24 hours after transplantation of S-180 cells. WSP was administered to the mice 5 times, every other day for 10 days. The doses were 320 mg/kg (10 mg/mouse) or 960 mg/kg (30 mg/mouse) of body weight. All mice were sacrificed 10 days after transplantation, and tumor growth was evaluated. The orally administered WSP significantly inhibited the growth of transplanted tumors (p < 0.05). Furthermore, histological findings revealed a significant reduction in mitotic cells and tumor invasion of the muscular tissue at both dose-levels of WSP.
Department of Radiology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.
Kohei
K
Inoue
Masayoshi
M
Saito
Takao
T
Kanai
Tetsuya
T
Kawata
Naoyuki
N
Shigematsu
Takashi
T
Uno
Kouichi
K
Isobe
Cui-Hua
CH
Liu
Hisao
H
Ito
D000818:Animals; D000890:Anti-Infective Agents / Q000494:pharmacology / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D008297:Male; D051379:Mice; D009368:Neoplasm Transplantation; D009372:Neoplasms, Connective Tissue / Q000188:drug therapy* / Q000473:pathology; D011429:Propolis / Q000494:pharmacology / Q000627:therapeutic use*; D012513:Sarcoma, Experimental / Q000188:drug therapy* / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
D000890:Anti-Infective Agents; D011429:Propolis