Leukotrienes and 8-isoprostane in exhaled breath condensate in bronchoprovocation tests with occupational allergens.
Exhaled breath condensate (EBC) contains many substances, which could help in diagnosis of occupational asthma. The aim of the study is to monitor leukotrienes (LT) and 8-isoprostane from EBC in bronchoprovocation tests with allergens in 47 patients with suspected occupational asthma. Forty-one patients were tested negative. In negative bronchoprovocation tests, no significant differences (P<0.05) were seen between the five measurements during and after the test. In control measurements (without provocation), significant differences were found among four measurements done within 24h for 8-isoprostane (P=0.0138). The relationship between the log transformed ratios of the EBC parameters and FEV(1) was never significant at the 5% level in control measurements, while in negative tests, statistical significance was recorded for LTB(4) (P=0.0299) before and 5h after the test. Six of 47 patients were tested positive. Such a small number of patients did not allow proper statistical analysis and therefore, the results are described separately for each patient.
Department of Occupational Medicine, 1st Faculty of Medicine, Charles University in Prague, Na Bojisti 1, Prague 2, Czech Republic. Pavlina.Janu@lf1.cuni.cz
Pavlina
P
Klusackova
Jindriska
J
Lebedova
Petr
P
Kacer
Marek
M
Kuzma
Marek
M
Brabec
Daniela
D
Pelclova
Zdenka
Z
Fenclova
Tomas
T
Navratil
D000328:Adult; D000395:Air Pollutants, Occupational / Q000276:immunology*; D000485:Allergens / Q000276:immunology*; D001944:Breath Tests; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis / Q000378:metabolism; D045853:Exhalation / Q000502:physiology*; D005260:Female; D006801:Humans; D015289:Leukotrienes / Q000032:analysis* / Q000378:metabolism; D008297:Male
D000395:Air Pollutants, Occupational; D000485:Allergens; D015289:Leukotrienes; C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost