Rhizobium miluonense sp. nov., a symbiotic bacterium isolated from Lespedeza root nodules.
Seventeen strains of lespedeza rhizobia, a previously defined novel group, were further characterized by comparison with phylogenetically related species using a polyphasic approach, including SDS-PAGE of whole-cell proteins, PCR-based restriction fragment length polymorphism analysis of ribosomal intergenic spacer sequences, determination of DNA G+C contents, DNA-DNA hybridization and analysis of phenotypic features. Based on data obtained in the present and previous studies, a novel species, Rhizobium miluonense sp. nov., is proposed for these strains. The type strain of R. miluonense is CCBAU 41251(T) (=LMG 24208(T)=HAMBI 2971(T)). Strain CCBAU 41251(T) could form ineffective nodules on Phaseolus vulgaris in addition to its original host Lespedeza chinensis.
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Chun Tao
CT
Gu
En Tao
ET
Wang
Chang Fu
CF
Tian
Tian Xu
TX
Han
Wen Feng
WF
Chen
Xin Hua
XH
Sui
Wen Xin
WX
Chen
D001426:Bacterial Proteins / Q000737:chemistry; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D004591:Electrophoresis, Polyacrylamide Gel; D020459:Genes, rRNA; D029884:Lespedeza / Q000382:microbiology*; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D018517:Plant Roots / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D012231:Rhizobium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013559:Symbiosis
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S