Cohesin protein SMC1 is a centrosomal protein.
Structural maintenance of chromosome protein 1 (SMC1) is well known for its roles in sister chromatid cohesion and DNA repair. In this study, we report a novel centrosomal localization of SMC1 within the cytoplasm in a variety of mammalian cell lines. We showed that SMC1 localized to centrosomes throughout the cell cycle in a microtubule-independent manner. Biochemically, SMC1 was cofractionated with the centrosomal protein gamma-tubulin in centrosomal preparation. Immunohistochemistry and immunoelectron microscopy performed on mouse tissue sections revealed that SMC1 antibody strongly labeled the base of cilia in ciliated epithelia, where basal bodies were located. Furthermore, we showed that SMC1 was associated with both centrioles of a centrosome at G0/G1 stage of the cell cycle. These results demonstrate that SMC1 is a centrosomal protein, suggesting possible involvement of SMC1 in centrosome/basal body-related functions, such as organization of dynamic arrays of microtubules and ciliary formation.
Department of Cell and Molecular Biology, Karolinska Institute, Berzelius väg 35, SE-171 77 Stockholm, Sweden.
Jikui
J
Guan
Emelie
E
Ekwurtzel
Ulrik
U
Kvist
Li
L
Yuan
D000818:Animals; D018797:Cell Cycle Proteins / Q000032:analysis / Q000378:metabolism*; D002460:Cell Line; D002502:Centrioles / Q000737:chemistry / Q000378:metabolism; D018385:Centrosome / Q000737:chemistry / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000032:analysis / Q000378:metabolism*; D004848:Epithelium / Q000378:metabolism; D006801:Humans; D051379:Mice; D000097722:Cohesins; D000098550:Structural Maintenance of Chromosome Protein 1
D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000097722:Cohesins; D000098550:Structural Maintenance of Chromosome Protein 1