Characterization of 19 novel cotton FLA genes and their expression profiling in fiber development and in response to phytohormones and salt stress.
Fasciclin-like arabinogalactan proteins (FLAs), a subclass of arabinogalactan proteins (AGPs), are usually involved in cell development in plants. To investigate the expression profiling as well as the role of FLA genes in fiber development, 19 GhFLA genes (cDNAs) were isolated from cotton (Gossypium hirsutum). Among them, 15 are predicted to be glycosylphosphatidylinositol anchored to the plasma membranes. The isolated cotton FLAs could be divided into four groups. Real-time quantitative reverse transcriptase polymerase chain reaction results indicated that the GhFLA genes are differentially expressed in cotton tissues. Three genes (GhFLA1/2/4) were specifically or predominantly expressed in 10 days post-anthesis fibers, and the transcripts of the other four genes (GhFLA6/14/15/18) were accumulated at relatively high levels in cotton fibers. Furthermore, expressions of the GhFLA genes are regulated in fiber development and in response to phytohormones and NaCl. The identification of cotton FLAs will facilitate the study of their roles in cotton fiber development and cell wall biogenesis.
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, Wuhan 430079, China.
Geng-Qing
GQ
Huang
Wen-Liang
WL
Xu
Si-Ying
SY
Gong
Bing
B
Li
Xiu-Lan
XL
Wang
Dan
D
Xu
Xue-Bao
XB
Li
D000595:Amino Acid Sequence; D032561:Cotton Fiber; D018076:DNA, Complementary / Q000737:chemistry / Q000235:genetics; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D003368:Gossypium / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D008969:Molecular Sequence Data; D009088:Mucoproteins / Q000145:classification / Q000235:genetics*; D010802:Phylogeny; D010937:Plant Growth Regulators / Q000494:pharmacology*; D010940:Plant Proteins / Q000145:classification / Q000235:genetics*; D020033:Protein Isoforms / Q000145:classification / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012965:Sodium Chloride / Q000494:pharmacology*
D018076:DNA, Complementary; D009088:Mucoproteins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D020033:Protein Isoforms; C006619:arabinogalactan proteins; D012965:Sodium Chloride