Hepatoprotective effect of Ginkgoselect Phytosome in rifampicin induced liver injury in rats: evidence of antioxidant activity.
The protective effects of Ginkgoselect Phytosome (GBP) on Rifampicin (RMP) induced hepatotoxicity and the probable mechanism(s) involved in this protection were investigated in rats. Liver damage was induced in Wistar rats by administering rifampicin (500 mg/kg, p.o.) daily for 30 days. Simultaneously, GBP at 25 mg/kg and 50 mg/kg, and the reference drug silymarin (100 mg/kg) were administered orally for 30 days/daily to RMP treated rats. Levels of marker enzymes (SGOT, SGPT and SALP), albaumin (Alb) and total proteins (TP) were assessed in serum. The effects of GBP on lipid peroxidation (LPO), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and glutathione reductase (GR) were assayed in liver homogenates to evaluate antioxidant activity. GBP (25 and 50 mg/kg) and silymarin elicited a significant hepatoprotective activity by lowering the levels of serum marker enzymes and lipid peroxidation and elevated the levels of GSH, SOD, CAT, GPX, GR, Alb and TP in a dose dependant manner. The present findings suggest that the hepatoprotective effect of GBP in RMP induced oxidative damage may be related to its antioxidant and free radical scavenging activity.
Naik
Suresh R
SR
Sinhgad Institute of Pharmaceutical Sciences, Kusgaon (Bk), Lonavala, Pune 410 401, India. srnaik5@rediffmail.com
Panda
Vandana S
VS
D000818:Animals; D000904:Antibiotics, Antitubercular; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D056486:Chemical and Drug Induced Liver Injury; D004798:Enzymes / Q000097:blood / Q000378:metabolism; D005260:Female; D005609:Free Radicals; D020441:Ginkgo biloba; D015227:Lipid Peroxidation / Q000187:drug effects*; D008099:Liver / Q000201:enzymology; D008107:Liver Diseases / Q000188:drug therapy*; D008297:Male; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar; D012293:Rifampin; D012838:Silymarin / Q000494:pharmacology / Q000627:therapeutic use
D000904:Antibiotics, Antitubercular; D000975:Antioxidants; D004798:Enzymes; D005609:Free Radicals; D010936:Plant Extracts; D012838:Silymarin; D012293:Rifampin