Glyoxylate induces renal tubular cell injury and microstructural changes in experimental mouse.
Crystal formation in mice could not be induced either by the administration of ethylene glycol or by glycolate. To clarify the reasons for the difference among these oxalate precursors in mice, we studied renal tubular epithelial injury by immunohistochemical staining of oxidative stress and observing microstructures. Daily intra-abdominal injection of saline solution [10 ml/(kg day)], ethylene glycol[(48.3 mmol/(kg day)], glycolate [1.31 mmol/(kg day)], and glyoxylate [1.35 mmol/(kg day)] into C57BL/6 male mice (8 weeks) was performed for 7 days. Immunohistochemical staining of superoxide dismutase (SOD) and malondialdehyde (MDA), and transmission electron microscopy (TEM) of renal tubular epithelial cells were performed to observe oxidative stress and morphological changes, respectively. Decreased SOD and increased MDA were shown only in glyoxylate-treated mouse kidneys. The TEM study with glyoxylate-treated mouse kidneys demonstrated that the internal structure of mitochondria in renal tubular cells underwent destruction and vacuolization, and microvilli density decreased. These changes in renal tubular cells were located in the crystal-forming area. However, such changes were not detected in the other groups. Each precursor of oxalate induces different changes in renal epithelial cells regarding oxidative stress and the microstructural changes. It is suggested that calcium oxalate crystal formation requires cell injury and morphological changes of renal epithelial tubular cells induced by glyoxylate administration in the mouse kidney.
Department of Nephro-Urology, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-Cho, Mizuho-ku, Nagoya City, Aichi, 467-8601, Japan.
Masahito
M
Hirose
Keiichi
K
Tozawa
Atsushi
A
Okada
Shuzo
S
Hamamoto
Hideo
H
Shimizu
Yasue
Y
Kubota
Yasunori
Y
Itoh
Takahiro
T
Yasui
Kenjiro
K
Kohri
D000818:Animals; D002129:Calcium Oxalate / Q000737:chemistry / Q000652:urine; D003460:Crystallization; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure*; D006038:Glyoxylates / Q000633:toxicity*; D007150:Immunohistochemistry; D007674:Kidney Diseases / Q000139:chemically induced* / Q000378:metabolism / Q000473:pathology*; D007684:Kidney Tubules / Q000187:drug effects / Q000378:metabolism / Q000473:pathology*; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism; D019459:Urothelium / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
D006038:Glyoxylates; D002129:Calcium Oxalate; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C031150:glyoxylic acid