Plasma and tissue vitamin E depletion in sheep with burn and smoke inhalation injury.
Oxidants are involved in the pathogenesis of many disorders caused by burn and smoke inhalation; alpha- and gamma-tocopherols are major tissue antioxidants, and their depletion should reflect oxidant injury. To determine whether plasma and tissue vitamin E levels would thus be depleted in severe burn, prepared sheep were randomly divided into the following groups: non-injured, burn- and smoke-exposed, burned only and smoke-exposed only. All were resuscitated with Ringer's lactate solution, mechanically ventilated and sacrificed at various time intervals. Immediately following injury plasma, lung, trachea, heart and liver tocopherols/lipids were measured and found to be significantly depleted except in the heart. Reduction of tissue gamma-tocopherol appeared earlier than reduction of alpha-tocopherol. Thus animals receiving combined burn and inhalation injury underwent marked oxidative stress, suggesting that vitamin E might be depleted also in humans with burn and smoke inhalation injury, and that appropriate supplementation should be evaluated.
Department of Plastic and Reconstructive Surgery, Tokyo Women's Medical University, Kawadacho 8-1, Shinjuku-ku, Tokyo 162-0054, Japan. kshimo420@ybb.ne.jp
Katsumi
K
Shimoda
Hiroaki
H
Nakazawa
Maret G
MG
Traber
Daniel L
DL
Traber
Motohiro
M
Nozaki
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002056:Burns / Q000150:complications / Q000378:metabolism*; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D055370:Lung Injury / Q000209:etiology / Q000378:metabolism*; D018384:Oxidative Stress; D012756:Sheep; D012906:Smoke / Q000009:adverse effects; D015208:Smoke Inhalation Injury / Q000209:etiology / Q000378:metabolism*; D014810:Vitamin E / Q000378:metabolism*; D024502:alpha-Tocopherol / Q000378:metabolism
D000975:Antioxidants; D012906:Smoke; D014810:Vitamin E; D024502:alpha-Tocopherol