A general target selection method for crystallographic proteomics.
Increasing the success in obtaining structures and maximizing the value of the structures determined are the two major goals of target selection in structural proteomics. This chapter presents an efficient and flexible target selection procedure supplemented with a Web-based resource that is suitable for small- to large-scale structural genomics projects that use crystallography as the major means of structure determination. Based on three criteria, biological significance, structural novelty, and "crystallizability," the approach first removes (filters) targets that do not meet minimal criteria and then ranks the remaining targets based on their "crystallizability" estimates. This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects.
Robin
Gautier
G
Institute for Molecular Bioscience, University of Queensland, Brisbane, Australia.
Cowieson
Nathan P
NP
Guncar
Gregor
G
Forwood
Jade K
JK
Listwan
Pawel
P
Hume
David A
DA
Kobe
Bostjan
B
Martin
Jennifer L
JL
Huber
Thomas
T
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D030562:Databases, Protein; D006801:Humans; D011506:Proteins / Q000737:chemistry*; D040681:Structural Homology, Protein
D011506:Proteins