[Investigation of fucoidanase by FTIR spectra].
Fucoidanase was isolated and purified from marine fungus LD8 by solid state fermentation, extraction with citric acid buffer, acetone precipitatation and column chromatography on Sephadex G-100. A single band on PAGE shows that pretty pure fucoidanase has been obtained. FT-IR spectra and its derivation, self-deconvolution and curve-fitting methods were used to analyze the secondary structure of the fucoidanase. Composite bands of the amide I and amide III were studied by using Fourier self-deconvolution (FSD) with an enhancement factor of K = 2.2 and a half width of 20.2 cm(-1). The relative average fractions of alpha-helix, beta-sheet, random coil, beta-turn are 11.5%, 58.6%, 14.5% and 15.9%, respectively, according to amide I region, while the content of alpha-helix is 12%, beta-sheet 57.3%, random coil 14.5%, and beta-turn 16.3% on amide III region. In other words, both the conclusions were exactly consistent. All the above results show that beta-sheet was the dominant component, which is about 58%, and that beta-turn is about 15%, random coil 15%, and alpha-helix 12% at room temperature.
College of Life Science, Anhui Agricultural University, Hefei 230036, China.
Shuang
S
Ma
Hou-Rong
HR
Xiao
Qian-Qian
QQ
Wu
Ren-Rui
RR
Pan
Jing-Min
JM
Cai
D005670:Fusarium / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000032:analysis* / Q000737:chemistry; D017433:Protein Structure, Secondary; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*
D006026:Glycoside Hydrolases