Dunning rat prostate adenocarcinomas and alternative splicing reporters: powerful tools to study epithelial plasticity in prostate tumors in vivo.
Using alternative splicing reporters we have previously observed mesenchymal epithelial transitions in Dunning AT3 rat prostate tumors. We demonstrate here that the Dunning DT and AT3 cells, which express epithelial and mesenchymal markers, respectively, represent an excellent model to study epithelial transitions since these cells recapitulate gene expression profiles observed during human prostate cancer progression. In this manuscript we also present the development of two new tools to study the epithelial transitions by imaging alternative splicing decisions: a bichromatic fluorescence reporter to evaluate epithelial transitions in culture and in vivo, and a luciferase reporter to visualize the distribution of mesenchymal epithelial transitions in vivo.
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Research Drive, Box 3053 (424 CARL), Durham, NC 27710, USA.
Sebastian
S
Oltean
Phillip G
PG
Febbo
Mariano A
MA
Garcia-Blanco
D000230:Adenocarcinoma / Q000235:genetics* / Q000473:pathology*; D017398:Alternative Splicing; D000818:Animals; D004847:Epithelial Cells / Q000473:pathology; D015870:Gene Expression; D020869:Gene Expression Profiling; D006801:Humans; D008164:Luminescent Proteins; D008297:Male; D008648:Mesoderm / Q000473:pathology; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology*; D051381:Rats; D051497:Receptor, Fibroblast Growth Factor, Type 2 / Q000235:genetics; D014407:Tumor Cells, Cultured
D008164:Luminescent Proteins; C496356:Fgfr2 protein, rat; D051497:Receptor, Fibroblast Growth Factor, Type 2