The untiring search for the most complete proteome representation: reviewing the methods.
Proteomic research has proved valuable for understanding the molecular mechanisms of biological processes, as well as in the search for biomarkers for a variety of diseases which lack a molecular diagnostic. While several new approaches are being developed, two-dimensional (2-DE) gel electrophoresis is still one of the most commonly used techniques, despite its many limitations. However, for biomarker research, 2-DE gel electrophoresis alone does not fulfill the necessary pre-requisites. If such a technique is utilized exclusively, a great part of a given proteome remains unseen. Therefore, very precise and sensitive techniques are needed. Here, we present a brief review of known methodologies that try to overcome the limitations of conventional proteome analysis as well as their respective advantages and limitations.
State University of Campinas, Brazil. java99@unicamp.br
Daniel
D
Martins de Souza
Bruno M
BM
Oliveira
Erich
E
Castro-Dias
Flavia V
FV
Winck
Ricardo S O
RS
Horiuchi
Paulo A
PA
Baldasso
Hugo T
HT
Caetano
Natália Katauchi Dayane
NK
Pires
Sergio
S
Marangoni
José C
JC
Novello
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D051379:Mice; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000302:isolation & purification; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012107:Research Design / Q000639:trends*; D012680:Sensitivity and Specificity
D009419:Nerve Tissue Proteins; D011506:Proteins; D020543:Proteome