[Determination of content of saikoside in Bupleurum Chinense from different sources].
OBJECTIVE
To determine the contents of saikosaponin a, d and saikosaponins in Radix Bupleuri from different and assess their quality.


METHODS
Saikosaponin a and d were used as the chemical reference substance to establish RP-HPLC and visible-spectrophotometry methods for determination of contents of saikosaponin a, d and saikosaponins.


RESULTS
The quality of B. chinense was better than that of B. scozonerifolium and B smishii; The contents of a, saikosaponin d and total saikosaponin in B. chinense were 0.100%-0.330%, 0.200%-0.400% and 1.00%-2.00% , respectively.


CONCLUSIONS
It is to suggest that the minimal contents requirements of saikosaponin a, d and saikosaponins in B. chinense should be set at 0. 100% , 0.200% and 1.00% , respectively; the best suitable planting areas are Taigu in the middle of Shanxi province, Ruicheng, Pinglu and Wanrong in the south of Shanxi province, and Longxi, Lixian in Gansu province.
School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
Yuan-Yuan
YY
Li
Xue-Mei
XM
Qin
Yu-Qing
YQ
Wang
Jian-Ying
JY
Yue
Jian-Ping
JP
Hao
D029972:Bupleurum / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000032:analysis / Q000737:chemistry; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D012503:Saponins; D009828:Oleanolic Acid; C025759:saikosaponin D