N-myc amplification was rarely detected by fluorescence in situ hybridization in retinoblastoma.
In retinoblastoma, genetic alteration of N-myc amplification different from the alteration of the RB1 gene on chromosome 13q14 has been described. This study is to determine the frequency of N-myc amplification by fluorescence in situ hybridization method in retinoblastoma. This study was prospectively derived from 26 patients who were diagnosed as having unilateral retinoblastoma (highly progressive large retinoblastoma, group 5 in Reese-Ellsworth classification) and underwent enucleation. We performed locus-specific fluorescence in situ hybridization probes for N-myc gene. Our results demonstrated that in only one of 26 patients was N-myc amplification found in retinoblastoma tissue. N-myc amplification has been regarded as one characteristic of retinoblastoma cell line and an adverse prognostic factor. However, our study indicates that N-myc amplification is not frequently found in retinoblastoma.
Department of Ophthalmology, College of Medicine, Seoul National University, 110-744 Seoul, South Korea.
Jeong Hun
JH
Kim
Jin Mi
JM
Choi
Young Suk
YS
Yu
Dong Hun
DH
Kim
Jin Hyoung
JH
Kim
Kyu-Won
KW
Kim
D000293:Adolescent; D000328:Adult; D002648:Child; D005134:Eye Neoplasms / Q000235:genetics*; D005260:Female; D005784:Gene Amplification; D016259:Genes, myc / Q000235:genetics*; D005819:Genetic Markers; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D012175:Retinoblastoma / Q000235:genetics*
D005819:Genetic Markers