Polarization anisotropy in fiber-optic second harmonic generation microscopy.
We report the investigation and implementation of a compact second harmonic generation microscope that uses a single-mode fiber coupler and a double-clad photonic crystal fiber. Second harmonic polarization anisotropy through the fiber-optic microscope systems is quantitatively measured with KTP microcrystals, fish scale and rat tail tendon. It is demonstrated that the polarized second harmonic signals can be excited and collected through the single-mode fiber coupler to analyze the molecular orientations of structural proteins. It has been discovered that a double-clad photonic crystal fiber can preserve the linear polarization in the core, although a depolarization effect is observed in the inner cladding region. The feasibility of polarization anisotropy measurements in fiber-optic second harmonic generation microscopy will benefit the in vivo study of collagen-related diseases with a compact imaging probe.
Fu
Ling
L
Centre for Micro-Photonics, Faculty of Engineering and Industrial Sciences, Swinburne University of Technology, P. O. Box 218, Hawthorn, Victoria 3122, Australia.
Gu
Min
M
D016880:Anisotropy; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation* / Q000379:methods*; D007089:Image Enhancement / Q000295:instrumentation*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods*; D055100:Optical Fibers