[Study on the genetic stability of immortalized cell line of lymphocyte cell transformed by EB virus after long subculture process].
OBJECTIVE
To study the genetic stability of an immortalized cell line transformed by Epstein-Barr virus (EBV) after long subculture process.


METHODS
In the present study, the genetic stability including chromosome diploidy, karyotypes and microsatellite DNA were evaluated with chromosome banding techniques and microsatellite DNA detection. The telomerase activity of the immortalized cell line was detected by using the telomerase assay kit.


RESULTS
From passage 1 to 30, there were no change of the diploidy, karyotypes of chromosome and microsatellite DNA, and the telomerase activity is negative.


CONCLUSIONS
This study indicates that the immortalized cell line remains stable genetically within limited passages.
Department of Medical Genetics, Institute of Biology and MedicineìCAMS and PUMC, Kunming, Yunnan, 650118 People' Republic of China.
Yan-han
YH
Li
Xiao-qin
XQ
Huang
Ke-qin
KQ
Lin
Yu-fen
YF
Tao
Wen
W
Yi
Yu-feng
YF
Yao
Lei
L
Shi
Jia-you
JY
Chu
D002472:Cell Transformation, Viral / Q000235:genetics*; D004854:Herpesvirus 4, Human / Q000235:genetics*; D006801:Humans; D008214:Lymphocytes / Q000166:cytology* / Q000378:metabolism / Q000821:virology*; D018895:Microsatellite Repeats / Q000235:genetics; D016133:Polymerase Chain Reaction