Uptake of postprandial lipoproteins into bone in vivo: impact on osteoblast function.
Dietary lipids and lipophilic vitamins are transported by postprandial lipoproteins and are required for bone metabolism. Despite that, it remains unknown whether bone cells are involved in the uptake of circulating postprandial lipoproteins in vivo. The current study was performed to investigate a putative participation of bone in the systemic postprandial lipoprotein metabolism in mice, to identify potentially involved cell type populations and to analyze whether lipoprotein uptake affects bone function in vivo. As a model for the postprandial state, chylomicron remnants (CR) were injected intravenously into mice. Next to the liver and compared to other organs, bone appeared to be the second most important organ for the clearance of radiolabeled CR particles from the circulation in vivo. In addition, uptake of radiolabeled CR by primary murine osteoblasts and hepatocytes was quantified to be in a similar range in vitro. A complementary approach with fluorescently labeled CR and immunohistochemical staining for apoE proved that intact CR particles were taken up into bone and liver. Electron microscopy localization studies of bone sections revealed CR uptake into sinusoidal endothelial cells, macrophages and osteoblasts. The relative amount of radiolabeled CR uptake into femoral cortical bone, representing predominantly osteoblasts, and bone marrow, representing predominantly non-osteoblast cells, was within the same range. Most importantly, the injection of vitamin K1-enriched CR resulted in an increase of the degree of osteocalcin carboxylation in vivo while total osteocalcin concentrations remained unaffected, giving functional proof that osteoblasts process CR in vivo. In conclusion, here we demonstrate that bone is involved in the postprandial lipoprotein metabolism in mice. Osteoblasts participate in CR clearance from the circulation, which has a direct impact on the secretory function of osteoblasts.
Niemeier
Andreas
A
Department of Orthopaedics, University Medical Center Hamburg-Eppendorf, Hamburg, Germany. Electronic address: niemeier@uke.uni-hamburg.de.
Niedzielska
Dagmara
D
Department of Biochemistry and Molecular Biology II: Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Secer
Rukiye
R
Department of Biochemistry and Molecular Biology II: Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Schilling
Arndt
A
Department of Trauma- Hand- and Reconstructive Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Merkel
Martin
M
Department of Internal Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
Enrich
Carlos
C
Departament de Biologia Cellular, Facultat de Medicina, Universitat de Barcelona, Spain.
Rensen
Patrick C N
PCN
Department of General Internal Medicine, Endocrinology and Metabolic Diseases, Leiden University Medical Center, The Netherlands.
Heeren
Joerg
J
Department of Biochemistry and Molecular Biology II: Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
D000818:Animals; D001842:Bone and Bones / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D002417:Cattle; D002478:Cells, Cultured; D002914:Chylomicrons / Q000378:metabolism / Q000648:ultrastructure; D022781:Hepatocytes / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D008074:Lipoproteins / Q000378:metabolism*; D008099:Liver / Q000166:cytology / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009928:Organ Specificity; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D015675:Osteocalcin / Q000097:blood; D019518:Postprandial Period; D010837:Vitamin K 1 / Q000378:metabolism
D002914:Chylomicrons; D008074:Lipoproteins; D015675:Osteocalcin; D010837:Vitamin K 1