Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
The expression of chemokine receptors and chemokine production by adult human non-transformed astrocytes, grade III astrocytoma and grade IV glioblastoma tumour cell lines were determined. Here, we show an increased expression of CXCR3 and CXCR4, and a decreased expression of CXCR1 and CCR4 by glioma cells compared to adult human astrocytes. Glioma cells showed increased production of CXCL10, whereas production of other chemokines was decreased (CXCL8, CCL2, CCL5, and CCL22). CXCL10 induced an ERK1/2-dependent increase in [(3)H] thymidine uptake. These results suggest that expression of chemokine receptor/ligand pairs such as CXCR3/CXCL10 have an important role in the proliferation of glioma cells.
Department of Life Sciences, The Open University, Walton Hall, Milton Keynes MK7 6AA, United Kingdom.
Seema V
SV
Maru
Karen A
KA
Holloway
Geraldine
G
Flynn
Christine L
CL
Lancashire
A Jane
AJ
Loughlin
David K
DK
Male
Ignacio A
IA
Romero
D001253:Astrocytes / Q000378:metabolism; D015153:Blotting, Western; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D054357:Chemokine CXCL10 / Q000096:biosynthesis*; D018925:Chemokines / Q000096:biosynthesis*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger; D019707:Receptors, Chemokine / Q000096:biosynthesis*; D020133:Reverse Transcriptase Polymerase Chain Reaction
C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D018925:Chemokines; D012333:RNA, Messenger; D019707:Receptors, Chemokine; D048049:Extracellular Signal-Regulated MAP Kinases