Antiproliferative effect of T/Tn specific Artocarpus lakoocha agglutinin (ALA) on human leukemic cells (Jurkat, U937, K562) and their imaging by QD-ALA nanoconjugate.
T/Tn specificity of Artocarpus lakoocha agglutinin (ALA), isolated from the seeds of A. lakoocha (Moraceae) fruit and a heterodimer (16 kD and 12 kD) of molecular mass 28 kD, was further confirmed by SPR analysis using T/Tn glycan containing mammalian glycoproteins. N-terminal amino acid sequence analysis of ALA showed homology at 15, 19-21, 24-27, and 29 residues with other lectin members of Moraceae family viz., Artocarpus integrifolia (jacalin) lectin, Artocarpus hirsuta lectin, and Maclura pomifera agglutinin. It is mitogenic to human PBMC and the maximum proliferation was observed at 1 ng/ml. It showed an antiproliferative effect on leukemic cells, with the highest effect toward Jurkat cells (IC(50) 13.15 ng/ml). Synthesized CdS quantum dot-ALA nanoconjugate was employed to detect the expression of T/Tn glycans on Jurkat, U937, and K562 leukemic cells surfaces as well as normal lymphocytes by fluorescence microscopy. No green fluorescence was observed with normal lymphocytes indicating that T/Tn determinants, which are recognized as human tumor associated structures were cryptic on normal lymphocyte surfaces, whereas intense green fluorescent dots appeared during imaging of leukemic cells, where such determinants were present in unmasked form. The above results indicated that QD-ALA nanoconjugate is an efficient fluorescent marker for identification of leukemic cell lines that gives rise to high quality images.
Department of Biological Chemistry, Indian Association for the Cultivation of Science, Jadavpur, Kolkata, 700 032, India.
Urmimala
U
Chatterjee
Partha Pratim
PP
Bose
Sharmistha
S
Dey
Tej P
TP
Singh
Bishnu P
BP
Chatterjee
D000595:Amino Acid Sequence; D031622:Artocarpus / Q000737:chemistry / Q000235:genetics; D002240:Carbohydrate Sequence; D049109:Cell Proliferation / Q000187:drug effects*; D006023:Glycoproteins / Q000737:chemistry; D006801:Humans; D019169:Jurkat Cells; D020014:K562 Cells; D007938:Leukemia / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D008934:Mitogens / Q000302:isolation & purification / Q000494:pharmacology; D008969:Molecular Sequence Data; D049329:Nanostructures; D009363:Neoplasm Proteins / Q000737:chemistry; D037121:Plant Lectins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D011134:Polysaccharides / Q000737:chemistry; D045663:Quantum Dots; D020298:U937 Cells
D006023:Glycoproteins; D008934:Mitogens; D009363:Neoplasm Proteins; D037121:Plant Lectins; D011134:Polysaccharides