Effects of BSO, GSH, Vit-C and DMPS on the nephrotoxicity of mercury.
OBJECTIVE
To study the effects of BSO, GSH, Vit-C and DMPS on the nephrotoxicity of mercury.


METHODS
The rats in groups 1, 2 and 3 were sc injected with 0.75, 1.5 and 2.5 mg/kg HgCl2, respectively. Fourth group rats were ip injected with 0.5 mmol/kg BSO and 4h later sc administrated with 0.75 mg/kg HgCl2. The rats in groups 5, 6 and 7 were ip injected with 3 mmol/kg GSH, 4 mmol/kg Vit-C, 200 micromol/kg DMPS, respectively, and 2 h later sc administrated with 2.5 mg/kg HgCl2. Eighth group rats were sc injected with saline as a control. Mercury concentrations in the liver, renal cortex and urine, urinary NAG, ALP, LDH activities, protein and BUN contents were determined.


RESULTS
Urinary NAG, ALP activities, protein and BUN contents in the rats of BSO pretreatment group were significantly higher than that of 0.75 mg/kg HgCl2 alone group and control group. As compared with 2.5 mg/kg HgCl2 alone group, urinary NAG, ALP, LDH activities, urinary protein and BUN contents decreased significantly.


CONCLUSIONS
BSO pretreatment could enhance the renal toxicity of mercury and GSH, Vit-C and DMPS pretreatment had antagonistic effects on nephrotoxicity of mercury.
Department of Environmental Health, School of Public Health, China Medical University, Shenyang, People's Republic of China. zfxu@mail.cmu.edu.cn
Zhaofa
Z
Xu
Jinghua
J
Yang
Jiaming
J
Yu
Zhongwei
Z
Yin
Wei
W
Sun
Jing
J
Li
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology*; D019328:Buthionine Sulfoximine / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004347:Drug Interactions; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005260:Female; D005978:Glutathione / Q000494:pharmacology*; D007672:Kidney Cortex / Q000187:drug effects; D007674:Kidney Diseases / Q000139:chemically induced* / Q000188:drug therapy / Q000378:metabolism; D008099:Liver / Q000187:drug effects; D008297:Male; D008627:Mercuric Chloride / Q000037:antagonists & inhibitors / Q000633:toxicity*; D008630:Mercury Poisoning / Q000188:drug therapy / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D014494:Unithiol / Q000494:pharmacology*
D000975:Antioxidants; D004791:Enzyme Inhibitors; D014494:Unithiol; D019328:Buthionine Sulfoximine; D008627:Mercuric Chloride; D005978:Glutathione; D001205:Ascorbic Acid