[The comparative analysis of the effect of oligopeptides and amino acids on the development of rat different tissue culture].
The effect the synthetic tetra-, tripeptides and amino acids, composed them, in concentration 10(-12) M was investigated in organotypic tissue culture on the cell proliferation and apoptosis development in six tissue explants (ecto-, meso- and entodermal genesis) in rats at age 3 months old. The tetrapeptide demonstrated the greater tissue-specificity, as compared to the tripeptides and amino acids. Each tetrapeptide stimulated proliferation in only one corresponded tissue type. Less tissue-specificity was observed in the tripeptides, which stimulated proliferation and apoptosis in one (one case)--four tissues. The amino acids stimulated proliferation and apoptosis in three--five tissues. It can be suggested, that tissue-specificity in these biologically active substances is depended on the structure complexity. It is discussed, that the tetrapeptide tissue-specificity is related to the complementar interaction to the DNA site-specific blocks.
Chalisova
N I
NI
Zakutskiĭ
A N
AN
Aniskina
A I
AI
Morozova
P Iu
PIu
D000596:Amino Acids / Q000494:pharmacology*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D003470:Culture Media; D004305:Dose-Response Relationship, Drug; D008297:Male; D009842:Oligopeptides / Q000494:pharmacology*; D009928:Organ Specificity; D051381:Rats; D017208:Rats, Wistar; D046509:Tissue Culture Techniques / Q000379:methods*
D000596:Amino Acids; D003470:Culture Media; D009842:Oligopeptides