Enzyme-linked immunosorbent assay for screening dioxin soil contamination by uncontrolled combustion during informal recycling in slums.
Uncontrolled combustion due to garbage recycling is a widespread activity among slum dwellers in distressed economy countries and has been indicated as a major source of dioxin contamination. However, because of the high cost and complexity of gas chromatography/high-resolution mass spectrometry (GC-HRMS) analysis, the magnitude of the problem remains largely unknown. The present study describes a first approach toward the use of a dioxin antibody-based enzyme-linked immunosorbent assay (ELISA) as the basis for a sustainable, simple, and low-cost monitoring program to assess the toxicological impact of uncontrolled combustion in slums. A panel of 16 samples was analyzed by GC-HRMS and ELISA on split extracts. Close to 20% of the analyzed samples showed dioxin concentrations up to almost twice the guidance level for residential soil in several countries, pointing out the need for performing a large-scale monitoring program. Despite the potential for variations in dioxin congener distribution due to the mixed nature of the incinerated material, there was a good correlation between the toxic equivalents as determined by GC-HRMS and ELISA. Furthermore, an interlaboratory ELISA validation showed that the capacity to perform the dioxin ELISA was successfully transferred between laboratories. It was concluded that the ELISA method performed very well as a screening tool to prioritize samples for instrumental analysis, which allows cutting down costs significantly.
Trindade
Mirta
M
Cátedra de Bioquímica, Department of Biosciences, Faculty of Chemistry, University de la República, Montevideo, Uruguay.
Nording
Malin
M
Nichkova
Mikaela
M
Spinnel
Erik
E
Haglund
Peter
P
Last
Michael S
MS
Gee
Shirley
S
Hammock
Bruce
B
Last
Jerold A
JA
González-Sapienza
Gualberto
G
Brena
Beatriz M
BM
D001572:Benzofurans / Q000032:analysis*; D000072338:Dibenzofurans, Polychlorinated; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D000072317:Polychlorinated Dibenzodioxins / Q000031:analogs & derivatives* / Q000032:analysis; D012989:Soil Pollutants / Q000032:analysis*
D001572:Benzofurans; D000072338:Dibenzofurans, Polychlorinated; D000072317:Polychlorinated Dibenzodioxins; D012989:Soil Pollutants