Fuchsin fluorescence in Mycobacterium tuberculosis: the Ziehl-Neelsen stain in a new light.
Tuberculosis (TB) is often diagnosed by observation of reddish pink fuchsin-stained Mycobacterium tuberculosis (MTB) in Ziehl-Neelsen (Z-N) stained smears by transmitted light microscopy. MTB too faintly stained with fuchsin to be seen by transmitted light may be detected by their green-excited orange-red fluorescence; this finding may be clinically relevant.
The Center for Microbial Cytometry and Howard M. Shapiro, M.D., P.C., 283 Highland Avenue, West Newton, MA 02465-2513, USA. hms@shapirolab.com
Howard M
HM
Shapiro
Thomas
T
Hänscheid
D005453:Fluorescence; D006801:Humans; D008856:Microscopy, Fluorescence; D009169:Mycobacterium tuberculosis / Q000166:cytology*; D012394:Rosaniline Dyes / Q000378:metabolism*; D013194:Staining and Labeling / Q000379:methods*; D014376:Tuberculosis / Q000175:diagnosis
D012394:Rosaniline Dyes