The effect of polysaccharide extracted from the marine alga Capsosiphon fulvescens on ethanol administration.
Seaweed extracts have recently been found to have antioxidant and antitumor activities. Capsosiphon fulvescens (Cf) is a green alga and nutrient-dense food source. In a previous study, we extracted polysaccharide from Cf (Cf-PS) and demonstrated its antitumor effect in gastric cancer cells. In this report, we describe the protective effect of Cf-PS against alcohol-induced gastric injury in rats and adenocarcinoma (AGS) cells. In vivo assay revealed stomach damage in rats treated with alcohol alone; however, the stomach condition of rats co-treated with Cf-PS and alcohol matched that of the control group. Cf-PS also inhibited alcohol-induced cell death in AGS cells. Compared with alcohol treatment alone, Cf-PS and alcohol co-treatment increased phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt but inhibited poly-ADP-ribose polymerase (PARP) cleavage. Thus, ERK1/2 and Akt activation are instrumental in the protective effect of Cf-PS against alcohol-induced cell death in AGS cells. Moreover, Cf-PS treatment reduced the expressions of cyclooxygenase-2 (COX-2) and the inducible form of nitric oxide (iNOS), proteins related to ulcers. These results suggest that Cf-PS could help protect against alcohol-induced peptic ulcers.
Hwang
Hye-Jeong
HJ
Department of Food Science and Biotechnology, Pukyong National University, Busan 608-737, Republic of Korea.
Kwon
Mi-Jin
MJ
Kim
In-Hye
IH
Nam
Taek-Jeong
TJ
D000818:Animals; D000897:Anti-Ulcer Agents; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002492:Central Nervous System Depressants / Q000633:toxicity*; D000460:Chlorophyta / Q000737:chemistry*; D004396:Coloring Agents; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D004789:Enzyme Activation / Q000187:drug effects; D000431:Ethanol / Q000633:toxicity*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005753:Gastric Mucosa / Q000473:pathology; D015870:Gene Expression / Q000187:drug effects; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D051058:Oncogene Protein v-akt / Q000378:metabolism; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013276:Stomach Ulcer / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*
D000897:Anti-Ulcer Agents; D002492:Central Nervous System Depressants; D004396:Coloring Agents; D011134:Polysaccharides; D000431:Ethanol; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D011065:Poly(ADP-ribose) Polymerases; D051058:Oncogene Protein v-akt; D048049:Extracellular Signal-Regulated MAP Kinases