[Comparative transcriptomic and proteomic analysis of the fetal and adult human liver].
The transcriptome and proteome comparative analysis of the fetal liver (9.5-10.5 weeks of gestation) and normal adult liver was developed in the present investigation. We used 44k microarrays of Agilent company and 2D-GE MALDI-TOF in transcriptome and proteome approaches, respectively. The top lists of expression genes and proteins for fetal liver obtained by these methods were compared. Transcriptome analysis confirmed proteome data only partially, but these two semi-quantitative approaches established the interdependences between expression levels of genes and proteins. The discrepancies between data obtained by two approaches were discussed. The unique feature of the fetal liver cell content is the combination of hemopoietic and nonmaturated liver cells. Fetal liver changes its hemopoietic role during embryogenesis towards the detoxicative tissue being able to metabolize different substances and produce wide serum of proteins. The status of fetal liver as the hemopoietic tissue was entirely characterized by transcriptome analysis having registered embryonic and adult hemoglobins, insufficient cytochrome-dependent detoxification system and highly developed anti-apoptotic potential.
L K
LK
Kurbatov
I B
IB
Cheglakov
K N
KN
Iarygin
G T
GT
Sukhikh
G V
GV
Vartanian
I Iu
IIu
Toropygin
A I
AI
Archakov
D000328:Adult; D015180:Electrophoresis, Gel, Two-Dimensional; D005333:Fetus; D020869:Gene Expression Profiling; D006801:Humans; D008099:Liver / Q000196:embryology / Q000378:metabolism*; D009024:Morphogenesis; D020411:Oligonucleotide Array Sequence Analysis; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D020543:Proteome