In vitro whole-organ imaging: 4D quantification of growing mouse limb buds.
Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).
Medical Research Council Human Genetics Unit, Crewe Road South, Edinburgh EH4 2XU, UK.
Marit J
MJ
Boot
C Henrik
CH
Westerberg
Juanjo
J
Sanz-Ezquerro
James
J
Cotterell
Ronen
R
Schweitzer
Miguel
M
Torres
James
J
Sharpe
D000818:Animals; D019521:Body Patterning; D005121:Extremities / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D021621:Imaging, Three-Dimensional / Q000379:methods*; D066298:In Vitro Techniques; D051379:Mice; D008822:Mice, Transgenic; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D041622:Tomography, Optical / Q000379:methods*
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins