Detection of circulating cancer cells in lung cancer patients with a panel of marker genes.
The current study was undertaken to examine the circulating cancer cells of lung cancer patients using a panel of markers and to evaluate the clinical significance of such tests. Peripheral blood mononuclear cells (PBMCs) from 134 lung cancer patients, 106 benign pulmonary disease, and 80 healthy individuals were isolated and assessed by nested reverse transcription-PCR assay for the expression of three different tumor markers, including tumor specific antigen 9 (TSA-9), Keratin 19 (KRT-19), and Pre-progastrin-releasing peptide (Pre-proGRP). Receiver operating characteristic curve (ROC) analysis showed that the combination of these markers was highly sensitive and specific in differentiating cancer patients from healthy and benign pulmonary disease controls. Of the 134 lung cancer patient blood samples, 84.3% expressed at least one tumor marker. A significant correlation was observed between the number of positive markers and disease stage and progression. Positivity of more than one marker predicted a poor response to therapy and short survival time in non-small cell lung cancer patients.
Liu
Lei
L
Department of Immunology, Peking University Health Science Center, 38 Xueyuan Road, Haidian District, Beijing 100083, China.
Liao
Guo-qing
GQ
He
Pei
P
Zhu
Hong
H
Liu
Peng-hui
PH
Qu
Yi-mei
YM
Song
Xiao-ming
XM
Xu
Qing-wen
QW
Gao
Qian
Q
Zhang
Yu
Y
Chen
Wei-feng
WF
Yin
Yan-hui
YH
D014408:Biomarkers, Tumor / Q000235:genetics*; D006801:Humans; D053539:Keratin-19 / Q000235:genetics; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics*; D009360:Neoplastic Cells, Circulating; D010455:Peptides / Q000235:genetics; D011379:Prognosis; D011498:Protein Precursors / Q000235:genetics; D012334:RNA, Neoplasm / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D014408:Biomarkers, Tumor; C530220:FAM83A protein, human; D053539:Keratin-19; D009363:Neoplasm Proteins; D010455:Peptides; D011498:Protein Precursors; D012334:RNA, Neoplasm; C047897:gastrin-releasing peptide precursor