Influence of interferons on the repair of UV-damaged DNA.
The capacity for nucleotide excision repair of a normal (WISH) and three tumour (MCF-7, HeLa, Namalva) cell lines treated with human recombinant interferons (hrIFN-alpha and hrIFN-gamma) was compared by the host cell reactivation assay. The cells were transfected with in vitro UV-damaged plasmid DNA (pEGFP-N1). The repair capacity was determined by measuring the fluorescence intensity of the expressed marker protein in total cell lysates. The correlation between the interferon-induced NO content and the suppressive effect of interferons on DNA repair was shown. The decrease of repair activity and NO induction by hrIFN-alpha were greatest in WISH, followed by MCF-7, Namalva and HeLa cells, whereas hrIFN-gamma was the best NO inducer and inhibitor for the repair of Namalva, followed by WISH, MCF-7 and HeLa cells. Our data clearly show that the two types of interferon have a strong inhibitory effect on the repair of UV-damaged DNA and this effect is cell type-dependent.
Tsoncheva
Vania L
VL
Institute of Molecular Biology, Bulgarian Academy of Sciences, 21 Acad. G. Bonchev str., 1113 Sofia, Bulgaria.
Todorova
Kristina A
KA
Ivanov
Ivan G
IG
Maximova
Vera A
VA
D001943:Breast Neoplasms; D002460:Cell Line; D004249:DNA Damage / Q000187:drug effects* / Q000528:radiation effects; D004260:DNA Repair / Q000187:drug effects*; D005260:Female; D006367:HeLa Cells / Q000528:radiation effects; D006801:Humans; D007372:Interferons / Q000494:pharmacology*; D009569:Nitric Oxide / Q000032:analysis; D010957:Plasmids / Q000187:drug effects; D014162:Transfection; D014466:Ultraviolet Rays; D002583:Uterine Cervical Neoplasms
D009569:Nitric Oxide; D007372:Interferons