Identification of differentially expressed proteins in the heart of translationally controlled tumor protein over-expressing transgenic mice.
We previously reported that transgenic (TG) mice over-expressing translationally controlled tumor protein (TCTP) developed systemic arterial hypertension at about 6 weeks after birth. In the present study, we identified, using proteomics technologies, 24 other proteins that were differentially expressed in the heart of TCTP over-expressing TG mice. These 24 proteins are involved in a variety of biological processes such as reactive oxygen species metabolism, cytoskeleton organization, fatty acid metabolism, amino acid metabolism and energy metabolism. We determined protein expression levels of the peroxiredoxin (Prx)2, Prx3, myosin light chain 1, stress protein (heat shock protein) 25K, and T-complex protein 1 alpha subunit by western blot analysis. Over-expression of TCTP probably regulates the expression of other proteins which play a pivotal role in a variety of cellular functions in TCTP over-expressing TG mice.
Cheon
Myeong Sook
MS
Department of Herbal Resources Research, Korea Institute of Oriental Medicine, Daejeon 305-811, Korea.
Suh
Jae-Kyung
JK
Kim
Min-Jeong
MJ
Kim
Seong Hwan
SH
Lee
Kyunglim
K
D000818:Animals; D014408:Biomarkers, Tumor / Q000032:analysis*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D015870:Gene Expression; D008297:Male; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008822:Mice, Transgenic; D009124:Muscle Proteins / Q000032:analysis*; D009206:Myocardium / Q000378:metabolism*; D000090324:Tumor Protein, Translationally-Controlled 1
D014408:Biomarkers, Tumor; D009124:Muscle Proteins; C000715648:Tpt1 protein, mouse; D000090324:Tumor Protein, Translationally-Controlled 1