Assembly pathway analysis of DNA nanostructures and the construction of parallel motifs.
We present a system for analyzing the assembly pathway of DNA nanostructures. This enables the identification, explanation, and avoidance of obstacles to proper structure formation. Potential problems include strand end-pinning and misfolding caused by the structural bias of nominally flexible junctions. We have used this system to guide the construction of parallel motifs that had previously, for unknown reasons, resisted assembly.
Center for Functional Nanomaterials, Brookhaven National Laboratory, Upton, P.O. Box 5000, New York 11973, USA.
Mudalige Thilak
MT
Kumara
Dmytro
D
Nykypanchuk
William B
WB
Sherman
D001483:Base Sequence; D004247:DNA / Q000032:analysis* / Q000737:chemistry* / Q000235:genetics / Q000648:ultrastructure; D007536:Isomerism; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009690:Nucleic Acid Conformation
D004247:DNA