Cellular recovery from electroporation using synchronisation modulation as a rescue model for electrically injured cells.
Electroporation of the plasma membrane resulting in a decrement in transmembrane potential is offered as a model in the study of the rescuing effects of the synchronisation modulation technique by electrically activating sodium potassium adenosine triphosphatase. Living cells were first electrically damaged by a pulsed intensive electric field, resulting in cell membrane electroporation, ion leakages and membrane potential depolarisation. Their recovery rate in natural conditions was compared with that of cells in a synchronisation modulation electric field. Fluorescence readings were taken using confocal microscopy and a potentiometric dye. Significantly more rapid recovery was observed after synchronisation modulation, with cell membranes actually polarised to levels higher than the original resting potential, a feature never seen in naturally recovering cells.
Laboratory for Cellular and Molecular Biophysics, Department of Physics, University of South Florida, Tampa, FL 33620, USA.
Robin
R
Dando
Wei
W
Chen
D000818:Animals; D002058:Burns, Electric / Q000473:pathology*; D002462:Cell Membrane / Q000502:physiology* / Q000528:radiation effects; D002463:Cell Membrane Permeability / Q000502:physiology; D004574:Electromagnetic Fields; D018274:Electroporation / Q000379:methods*; D007477:Ions / Q000378:metabolism; D008564:Membrane Potentials / Q000502:physiology* / Q000528:radiation effects; D008856:Microscopy, Fluorescence; D023421:Models, Animal; D018485:Muscle Fibers, Skeletal / Q000378:metabolism; D011894:Rana pipiens; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism*
D007477:Ions; D000254:Sodium-Potassium-Exchanging ATPase