Selenium stimulates pancreatic beta-cell gene expression and enhances islet function.
The present study investigated the role of selenium in the regulation of pancreatic beta-cell function. Utilising the mouse beta-cell line Min6, we have shown that selenium specifically upregulates Ipf1 (insulin promoter factor 1) gene expression, activating the -2715 to -1960 section of the Ipf1 gene promoter. Selenium increased both Ipf1 and insulin mRNA levels in Min6 cells and stimulated increases in insulin content and insulin secretion in isolated primary rat islets of Langerhans. These data are the first to implicate selenium in the regulation of specific beta-cell target genes and suggest that selenium potentially promotes an overall improvement in islet function.
Institute for Cell and Molecular Biosciences, Newcastle University, UK.
Susan C
SC
Campbell
Ali
A
Aldibbiat
Claire E
CE
Marriott
Caroline
C
Landy
Tomader
T
Ali
William F
WF
Ferris
Clive S
CS
Butler
James A
JA
Shaw
Wendy M
WM
Macfarlane
D000818:Animals; D002478:Cells, Cultured; D015870:Gene Expression / Q000187:drug effects; D018398:Homeodomain Proteins / Q000235:genetics*; D007328:Insulin / Q000235:genetics* / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D064586:Selenic Acid; D012643:Selenium / Q000378:metabolism* / Q000494:pharmacology; D018036:Selenium Compounds / Q000494:pharmacology; D015534:Trans-Activators / Q000235:genetics*
D018398:Homeodomain Proteins; D007328:Insulin; D012333:RNA, Messenger; D018036:Selenium Compounds; D015534:Trans-Activators; C084010:pancreatic and duodenal homeobox 1 protein; D012643:Selenium; D064586:Selenic Acid