[Involvement of nitric oxide and other molecules with redox potential in primary open angle glaucoma].
OBJECTIVE
Nitric oxide (NO) and other molecules with redox potential are involved in cell signalling, including endothelial-dependent relaxation and the maintenance of vascular homeostasis. We investigated the availability of NO and the formation of reactive oxygen species (ROS) in the aqueous humour and its relationship to the pathogenic mechanisms of primary open-angle glaucoma (POAG).


METHODS
We analysed biochemically aqueous humour samples from patients having anterior segment surgery that were divided into two separate groups: 1) patients having a Watson's trabeculectomy because of worsening of the glaucoma evolution (GG; n=60), and 2) a comparative group of individuals having phacoemulsification for non-complicated cataracts (CG; n=60). Enzymatic-colorimetric methods were used to analyse the NO concentration, the lipid peroxidation by-product malondialdehyde (MDA) and the total antioxidant status (TAS). Statistical analysis was carried out in the two groups by means of the SPSS 15.0 programme.


RESULTS
A significant increase in both the NO concentration (p<0.05) and MDA levels (p<0.05), and a significant decrease of the TAS (p<0.05) were detected in the GG versus the CG samples.


CONCLUSIONS
Changes in NO availability and the concomitance of oxidative stress in the aqueous humour of the POAG patients may be useful markers for identifying those at risk of glaucoma progression and visual loss.
Universidad de Valencia, Unidad de Investigación Oftalmológica Santiago Grisolía, Hospital Universitario Dr. Peset, Valencia, España. pinazo_mar@gva.es
V
V
Zanón-Moreno
S
S
Pons
R
R
Gallego-Pinazo
J
J
García-Medina
I
I
Vinuesa
V
V
Vila Bou
M D
MD
Pinazo-Durán
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000737:chemistry*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009569:Nitric Oxide / Q000032:analysis* / Q000502:physiology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D017382:Reactive Oxygen Species; D009569:Nitric Oxide