The development of multiple probe microdialysis sampling in the stomach.
A multiple probe approach of implanting microdialysis probes into each separate tissue layer would better represent sampling from the stomach. Presently, microdialysis sampling experiments are performed with only a single probe in the submucosa to represent sampling from the stomach tissue. The focus of this research was to develop a four-probe microdialysis sampling design to simultaneously monitor the stomach lumen, mucosa, submucosa and in the blood of the rat. Due to the small outer diameter of the microdialysis probe (350 microm), implantation into each separate layer was achieved with confirmation of probe location from histological examination. To assess the significance of sampling by this approach, multiple probe microdialysis sampling was used to monitor drug absorption in the stomach. Salicylic acid, caffeine and metoprolol were individually dosed to the ligated stomach. Analysis of the dialysate samples was performed by HPLC-UV and concentration-time curves and pharmacokinetics analysis were used to determine differences between the different probe locations.
R.N. Adams Institute for Bioanalytical Chemistry, University of Kansas, 2030 Becker Drive, Lawrence, KS 66047, USA.
Kristin L
KL
Woo
Craig E
CE
Lunte
D000042:Absorption; D000818:Animals; D019540:Area Under Curve; D002110:Caffeine / Q000097:blood / Q000493:pharmacokinetics; D005260:Female; D005753:Gastric Mucosa / Q000098:blood supply* / Q000378:metabolism*; D007601:Jugular Veins; D008657:Metabolic Clearance Rate; D008790:Metoprolol / Q000097:blood / Q000493:pharmacokinetics; D017551:Microdialysis / Q000295:instrumentation* / Q000379:methods*; D051381:Rats; D017207:Rats, Sprague-Dawley; D020156:Salicylic Acid / Q000097:blood / Q000493:pharmacokinetics; D013270:Stomach / Q000098:blood supply*
D002110:Caffeine; D008790:Metoprolol; D020156:Salicylic Acid