PsL-EGFmAb inhibits the stimulatory functions of human dendritic cells via DC-SIGN.
Dendritic cell (DC)-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is a DC-specific C-type lectin that plays an important role in recognizing and capturing pathogens, DC migration and initiation of T cell responses. Here, we show that anti-P-selectin lectin-EGF domain monoclonal antibody (PsL-EGFmAb), originally prepared for blockade of the adhesive molecule P-selectin, significantly down-regulated DC-SIGN expression as well as expression of mature DC-related molecules including CD83, CD86 and CD80 on human DCs. This PsL-EGFmAb treatment of DCs resulted in impaired allogeneic T cell proliferation and IL-12 production. Furthermore, we show that PsL-EGFmAb-induced down-regulation of DC-SIGN may inhibit NF-kappaB expression in DCs, which accounts for the inhibition of DC maturation and stimulatory function. Our present studies indicate that PsL-EGFmAb may be a useful reagent for regulating DC-SIGN expression and DC function.
Department of Nephrology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Rui jin Er road, Shanghai 200025, China. zhoutong_cn@hotmail.com
Tong
T
Zhou
Yanyun
Y
Zhang
Guizhi
G
Sun
Jie
J
Zou
Xiao
X
Li
Minchao
M
Cai
Yichuan
Y
Xiao
Yumei
Y
Zhang
Yapeng
Y
Zhao
Nan
N
Chen
D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D015703:Antigens, CD / Q000276:immunology; D018949:Antigens, CD1 / Q000276:immunology; D015815:Cell Adhesion Molecules / Q000276:immunology*; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D004815:Epidermal Growth Factor / Q000276:immunology; D005312:Fetal Blood / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D006684:HLA-DR Antigens / Q000276:immunology; D006801:Humans; D037181:Lectins, C-Type / Q000276:immunology*; D009687:Nuclear Proteins / Q000276:immunology; D011956:Receptors, Cell Surface / Q000276:immunology*; D012016:Reference Values; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D000911:Antibodies, Monoclonal; D015703:Antigens, CD; D018949:Antigens, CD1; D015815:Cell Adhesion Molecules; D004815:Epidermal Growth Factor; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D006684:HLA-DR Antigens; D037181:Lectins, C-Type; D009687:Nuclear Proteins; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C419393:CD1a antigen; C044854:nuclear antigen p55, human