Determination of trace lead in biological and water samples with dispersive liquid-liquid microextraction preconcentration.
A new method for the determination of trace lead was developed by dispersive liquid-liquid microextraction preconcentration and graphite furnace atomic absorption spectrometry. In the proposed approach, 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone (PMBP) was used as a chelating agent, and carbon tetrachloride and ethanol were selected as extraction and dispersive solvents. Some factors influencing the extraction efficiency of lead and its subsequent determination, including extraction and dispersive solvent type and volume, pH of sample solution, concentration of the chelating agent, and extraction time, were studied and optimized. Under the optimum conditions, the enrichment factor of this method for lead was reached at 78. The detection limit for lead was 39 ng L(-1) (3 sigma), and the relative standard deviation (RSD) was 3.2% (n=7, c=10 ng mL(-1)). The method was successfully applied to the determination of trace amounts of lead in human urine and water samples.
Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, People's Republic of China. liangpei@mail.ccnu.edu.cn
Pei
P
Liang
Hongbo
H
Sang
D000042:Absorption; D053000:Analytic Sample Preparation Methods / Q000379:methods*; D006197:Hair / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007854:Lead / Q000032:analysis* / Q000302:isolation & purification* / Q000652:urine; D047069:Pyrazolones / Q000737:chemistry; D015203:Reproducibility of Results; D012997:Solvents / Q000737:chemistry; D013057:Spectrum Analysis; D013696:Temperature; D013997:Time Factors; D014867:Water / Q000737:chemistry*
D047069:Pyrazolones; D012997:Solvents; D014867:Water; D007854:Lead; C038362:pyrazolone