Heterologous production of secondary metabolites as pharmaceuticals in Saccharomyces cerevisiae.
Heterologous expression of genes involved in the biosynthesis of various products is of increasing interest in biotechnology and in drug research and development. Microbial cells are most appropriate for this purpose. Availability of more microbial genomic sequences in recent years has greatly facilitated the elucidation of metabolic and regulatory networks and helped gain overproduction of desired metabolites or create novel production of commercially important compounds. Saccharomyces cerevisiae, as one of the most intensely studied eukaryotic model organisms with a rich density of knowledge detailing its genetics, biochemistry, physiology, and large-scale fermentation performance, can be capitalized upon to enable a substantial increase in the industrial application of this yeast. In this review, we describe recent efforts made to produce commercial secondary metabolites in Saccharomyces cerevisiae as pharmaceuticals. As natural products are increasingly becoming the center of attention of the pharmaceutical and nutraceutical industries, such as naringenin, coumarate, artemisinin, taxol, amorphadiene and vitamin C, the use of S. cerevisiae for their production is only expected to expand in the future, further allowing the biosynthesis of novel molecular structures with unique properties.
Huang
Beibei
B
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China.
Guo
Jing
J
Yi
Bo
B
Yu
Xiaojing
X
Sun
Lianna
L
Chen
Wansheng
W
D037621:Artemisinins / Q000378:metabolism; D001205:Ascorbic Acid / Q000096:biosynthesis; D003373:Coumaric Acids / Q000378:metabolism; D004345:Drug Industry / Q000379:methods*; D044950:Flavanones / Q000096:biosynthesis; D007783:Lactones / Q000378:metabolism; D017239:Paclitaxel / Q000096:biosynthesis; D004364:Pharmaceutical Preparations; D000081223:Polycyclic Sesquiterpenes; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D012717:Sesquiterpenes / Q000378:metabolism
D037621:Artemisinins; D003373:Coumaric Acids; D044950:Flavanones; D007783:Lactones; D004364:Pharmaceutical Preparations; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C515348:amorpha-4,11-diene; C005273:naringenin; D017239:Paclitaxel; D001205:Ascorbic Acid; C075875:artemisin