Can PAHs influence Cu accumulation by salt marsh plants?
The presence of polycyclic aromatic hydrocarbons (PAHs) may change the mechanisms of metal uptake, thus influencing kinetics and extent of metal phytoextraction. Studies on the subject are scarce, particularly for salt marsh plants. The aim of this work was to investigate the effect of PAHs on the uptake of Cu by Halimione portulacoides, a plant commonly found in salt marshes. Experiments were carried out in the laboratory, either in hydroponics (sediment elutriate) or in sediment soaked in elutriate, which were prepared with sediment and water from a salt marsh of the Cavado river estuary (NW Portugal). Groups of H. portulacoides (grown in a greenhouse) were exposed to those media during six days. Cu2+ (as Cu(NO3)2), 10(2) and 10(4) microg l(-1), was added to the media as well as 1.6 microg l(-1) of the sixteen EPA priority PAHs (0.1 microg l(-1) of each PAHs). Cu was assayed in solutions, sediments and different plant tissues before and after experiments. After exposure, photosynthetic efficiency and levels of chlorophylls were also measured, indicating that plant stress indicators were identical in all plants independently of the media to which the plants were exposed. PAHs influenced both the soluble Cu fraction and Cu uptake by plants. The amounts of metal accumulated in both roots and stems were significantly higher when the 10(4) microg l(-1) of Cu enriched elutriate was amended with PAHs. Thus, results suggest that PAHs may modify Cu solubility, the Cu sorption by plants and/or the passive penetration of Cu into the root cells. Therefore, the combined effects of different types of pollutants should be taken in consideration when studying the remediation potential of plants, namely in terms of phytoextraction.
CIMAR/CIIMAR-Centro Interdisciplinar de Investigação Marinha e Ambiental, Universidade do Porto, Rua dos Bragas, 289, 4050-123 Porto, Portugal. calmeida@fc.up.pt
C Marisa R
CM
Almeida
Ana P
AP
Mucha
Marta F C
MF
Delgado
M Isabel
MI
Caçador
A A
AA
Bordalo
M Teresa S D
MT
Vasconcelos
D000327:Adsorption; D027703:Amaranthaceae / Q000187:drug effects* / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D003300:Copper / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D052918:Environmental Restoration and Remediation; D019015:Geologic Sediments / Q000737:chemistry; D010788:Photosynthesis / Q000187:drug effects; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018547:Plant Stems / Q000187:drug effects / Q000378:metabolism; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*; D053833:Wetlands
D011084:Polycyclic Aromatic Hydrocarbons; D014874:Water Pollutants, Chemical; D002734:Chlorophyll; D003300:Copper