Probing the interaction between peptides and metal oxides using point mutants of a TiO2-binding peptide.
An increasing number of peptides with specific binding affinity to inorganic materials are being isolated using combinatorial peptide libraries without prior knowledge about the interaction between peptides and target materials. The lack of understanding of the mechanism and the contribution of constituent amino acids to the peptides' inorganic-binding ability poses an obstacle to optimizing and tuning of the binding affinity of peptides to inorganic materials and thus hinders the practical application of these peptides. Using the phage surface display technique, we previously identified a disulfide-bond-constrained peptide (-CHKKPSKSC-, STB1) cognitive of TiO2. In the present study, the interaction of STB1 with TiO2 was probed using a series of point mutants of STB1 displayed on phage surfaces. Their binding affinity was measured using a quartz crystal microbalance with energy dissipation measurement and compared on the basis of the delta f or delta D values. The three K residues of STB1 were found to be essential and sufficient for phage particle binding to TiO2. One mutant with five K residues showed not stronger but weaker binding affinity than STB1 due to its conformational restriction, as illustrated by molecular dynamics simulation, to align five K residues in a way conducive to their simultaneous interaction with the TiO2 surface. The contextual influence of noncharged residues on STB1's binding affinity was also investigated. Our results may provide insight into the electrostatic interaction between peptides and inorganic surfaces.
Department of Chemical & Biomolecular Engineering, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260.
Haibin
H
Chen
Xiaodi
X
Su
Koon-Gee
KG
Neoh
Woo-Seok
WS
Choe
D000595:Amino Acid Sequence; D008670:Metals / Q000737:chemistry*; D008958:Models, Molecular; D010087:Oxides / Q000737:chemistry*; D019151:Peptide Library; D017354:Point Mutation / Q000235:genetics; D014025:Titanium / Q000737:chemistry*
D008670:Metals; D010087:Oxides; D019151:Peptide Library; C009495:titanium dioxide; D014025:Titanium