Phenotypic and genotypic analyses of Neisseria gonorrhoeae isolates that express frequently recovered PorB PIA variable region types suggest that certain P1a porin sequences confer a selective advantage for urogenital tract infection.
Typing of the porB variable region (VR) is an epidemiological tool that classifies gonococcal strains based on sequence differences in regions of the porB gene that encode surface-exposed loops. The frequent isolation of certain porB VR types suggests that some porin sequences confer a selective advantage during infection and/or transmission. Alternatively, certain porin types may be markers of strains that are successful due to factors unrelated to porin. In support of the first hypothesis, here we show urogenital tract isolates representing the most common PIA VR types identified in an urban clinic in Baltimore, MD, over a 10-year period belonged to several different clonal types, as determined by pulsed-field gel electrophoresis (PFGE). Serum resistance, which was confirmed by factor H and C4b-binding protein binding studies, was more often associated with gonococcal the most common VR types. In contrast, three porin-independent phenotypes, namely, lactoferrin utilization, beta-lactamase production, and multiple transferable resistance (Mtr), were segregated with the PFGE cluster and not with the VR type. Data combined with another PIA strain collection showed a strong correlation between serum resistance and the most common VR types. A comparison of VR typing hybridization patterns and nucleotide sequences of 12 porB1a genes suggests that certain porin loop 1, 3, 6, and/or 7 sequences may play a role in the serum resistance phenotype. We conclude that some PorB PIA sequences confer a survival or transmission advantage in the urogenital tract, perhaps via increased resistance to complement-mediated killing. The capacity of some porin types to evade a porin-specific adaptive immune response must also be considered.
Garvin
Lotisha E
LE
Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd., Bethesda, MD 20853-4799, USA.
Bash
Margaret C
MC
Keys
Christine
C
Warner
Douglas M
DM
Ram
Sanjay
S
Shafer
William M
WM
Jerse
Ann E
AE
D015373:Bacterial Typing Techniques; D015142:Baltimore / Q000453:epidemiology; D001770:Blood Bactericidal Activity; D016000:Cluster Analysis; D050716:Complement C4b-Binding Protein / Q000378:metabolism; D017242:Complement Factor H / Q000378:metabolism; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D052776:Female Urogenital Diseases / Q000453:epidemiology / Q000382:microbiology*; D005838:Genotype; D006069:Gonorrhea / Q000453:epidemiology* / Q000382:microbiology; D006801:Humans; D007781:Lactoferrin / Q000378:metabolism; D008297:Male; D052801:Male Urogenital Diseases / Q000453:epidemiology / Q000382:microbiology*; D020409:Molecular Motor Proteins; D009344:Neisseria gonorrhoeae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D011110:Polymorphism, Genetic; D018272:Porins / Q000235:genetics*; D011485:Protein Binding; D014505:Urban Population; D001618:beta-Lactamases / Q000378:metabolism
D050716:Complement C4b-Binding Protein; D004269:DNA, Bacterial; D020409:Molecular Motor Proteins; D018272:Porins; C057699:porin protein, Neisseria; D017242:Complement Factor H; D007781:Lactoferrin; D001618:beta-Lactamases