Structure-activity investigation on the gene transfection properties of cardiolipin mimicking gemini lipid analogues.
A structure-activity relationship has been explored on the gene transfection efficiencies of cardiolipin mimicking gemini lipid analogues upon variation of length and hydrophilicity of the spacer between the cationic ammonium headgroups and lipid hydrocarbon chain lengths. All the gemini lipids were found to be highly superior in gene transfer abilities as compared to their monomeric lipid and a related commercially available formulation. Pseudoglyceryl gemini lipids bearing an oxyethylene (-CH2-(CH2-O-CH2)m-CH2-) spacer were found to be superior gene transfecting agents as compared to those bearing polymethylene (-CH2)m-) spacers. The major characteristic feature of the present set of gemini lipids is their serum compatibility, which is most often the major hurdle in liposome-mediated gene delivery.
Department of Organic Chemistry, Indian Institute of Science, Bangalore 560 012, India.
Avinash
A
Bajaj
Bishwajit
B
Paul
Paturu
P
Kondaiah
Santanu
S
Bhattacharya
D040761:Biomimetic Materials / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D002308:Cardiolipins / Q000737:chemistry*; D004247:DNA / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008081:Liposomes / Q000737:chemistry; D008854:Microscopy, Electron; D010714:Phosphatidylethanolamines / Q000737:chemistry; D000644:Quaternary Ammonium Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism*; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D013329:Structure-Activity Relationship; D013499:Surface Properties; D014162:Transfection / Q000379:methods*; D044366:Transition Temperature; D014867:Water / Q000737:chemistry
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D002308:Cardiolipins; D008081:Liposomes; D010714:Phosphatidylethanolamines; D000644:Quaternary Ammonium Compounds; D014867:Water; C054005:N-(1-(2,3-dioleyloxy)propyl)-N,N,N-trimethylammonium; D012967:Sodium Dodecyl Sulfate; D004247:DNA