Imaging collagen remodeling and sensing transplanted autologous fibroblast metabolism in mouse dermis using multimode nonlinear optical imaging.
Collagen remodeling and transplanted autologous fibroblast metabolic states in mouse dermis after cellular injection are investigated using multimode nonlinear optical imaging. Our findings show that the technique can image the progress of collagen remodeling in mouse dermis. It can also image transplanted autologous fibroblasts in their collagen matrix environment in the dermis, because of metabolic activity. It was also found that the approach can provide two-photon ratiometric redox fluorometry based on autologous fibroblast fluorescence from reduced nicotinamide adenine dinucleotide coenzyme and oxidized flavoproteins for sensing the autologous fibroblast metabolic state. These results show that the multimode nonlinear optical imaging technique may have potential in a clinical setting as an in vivo diagnostic and monitoring system for cellular therapy in plastic surgery.
Zhuo
Shuangmu
S
Key Laboratory of Optoelectronic Science and Technology for Medicine (Fujian Normal University), Ministry of Education, Fuzhou 350007, People's Republic of China.
Chen
Jianxin
J
Cao
Ning
N
Jiang
Xingshan
X
Xie
Shusen
S
Xiong
Shuyuan
S
D000818:Animals; D002417:Cattle; D003094:Collagen / Q000378:metabolism*; D020405:Dermis / Q000166:cytology* / Q000378:metabolism* / Q000637:transplantation; D004848:Epithelium / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism* / Q000637:transplantation*; D005453:Fluorescence; D016014:Linear Models; D051379:Mice; D055095:Optics and Photonics; D017785:Photons; D015203:Reproducibility of Results; D023822:Tissue Engineering; D014182:Transplantation, Autologous
D003094:Collagen