Structural genomics of pathogenic protozoa: an overview.
The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.
Department of Biochemistry, University of Washington, Seattle, WA, USA.
Erkang
E
Fan
David
D
Baker
Stanley
S
Fields
Michael H
MH
Gelb
Frederick S
FS
Buckner
Wesley C
WC
Van Voorhis
Eric
E
Phizicky
Mark
M
Dumont
Christopher
C
Mehlin
Elizabeth
E
Grayhack
Mark
M
Sullivan
Christophe
C
Verlinde
George
G
Detitta
Deirdre R
DR
Meldrum
Ethan A
EA
Merritt
Thomas
T
Earnest
Michael
M
Soltis
Frank
F
Zucker
Peter J
PJ
Myler
Lori
L
Schoenfeld
David
D
Kim
Liz
L
Worthey
Doug
D
Lacount
Marissa
M
Vignali
Jizhen
J
Li
Somnath
S
Mondal
Archna
A
Massey
Brian
B
Carroll
Stacey
S
Gulde
Joseph
J
Luft
Larry
L
Desoto
Mark
M
Holl
Jonathan
J
Caruthers
Jürgen
J
Bosch
Mark
M
Robien
Tracy
T
Arakaki
Margaret
M
Holmes
Isolde
I
Le Trong
Wim G J
WG
Hol
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray / Q000379:methods; D023281:Genomics / Q000379:methods*; D010961:Plasmodium / Q000235:genetics*; D015800:Protozoan Proteins / Q000737:chemistry*; D014351:Trypanosomatina / Q000235:genetics*
D015800:Protozoan Proteins