[The preliminary analysis of the recognition epitopes of anti-HEV monoclonal antibodies on HEV ORF2].
Western blot, capture-PCR, blocking ELISA and synthetic polypeptides were used to systematically study the recognition epitopes on HEV ORF2 of 23 anti-HEV monoclonal antibodies(McAbs) which were previously generated in our laboratory directed against HEV ORF2. Results showed that seven McAbs recognized linear epitopes that located at aa408-458 of HEV ORF2 and 16 conformation-dependent McAbs, most of which recognized the surface epitopes of native HEV, located at aa459-606 of HEV ORF2. The systematical study of the recognition epitopes of anti-HEV McAbs on HEV ORF2 provides important information for the investigation of virus receptor and HEV infection mechanism, as well as its vaccine and diagnostics development.
National Institute of Diagnostics and Vaccine Development of Infectious Disease, College of Life Science of Xiamen University, Xiamen 361005, China.
Jun-Hui
JH
Xiong
Qing-Shun
QS
Guo
Sheng-Xiang
SX
Ge
Ying
Y
Gu
Yi-Xin
YX
Chen
Ji
J
Miao
Hai-Lian
HL
Du
Wei-Guo
WG
Shi
Jun
J
Zhang
Ning-Shao
NS
Xia
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes; D006508:Hepatitis Antibodies / Q000276:immunology*; D016752:Hepatitis E virus / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D014764:Viral Proteins / Q000276:immunology*
D000911:Antibodies, Monoclonal; D000939:Epitopes; D006508:Hepatitis Antibodies; C400971:ORF2 protein, Hepatitis E virus; D014764:Viral Proteins