Neuromuscular changes in a rat model of colitis.
Intracolonic administration of Trichinella spiralis larvae in rats causes colitis with features similar to ulcerative colitis, notably with inflammation predominantly limited to the colonic mucosa. Our aim was to characterize the functional and neurochemical changes occurring within the myenteric (MP) and submucosal plexuses (SMP) during T. spiralis-induced colitis. Infected rats had decreased body weight, altered stool consistency and elevated myeloperoxidase activity, 6 and 14 days post-infection (PI). Responses to acetylcholine and KCl in circular muscle strips were reduced in infected tissues, demonstrating an impairment of contractility. In addition, there was a decrease in spontaneous motor activity and reduced sensitivity to the nitric oxide synthase (NOS) inhibitor L-NOArg, corresponding with a significant reduction in NOS immunoreactive neurons in the MP of infected animals. T. spiralis did not alter the total number of myenteric or submucosal neurons. Substance P innervation of submucosal blood vessels was reduced after infection, as were submucosal calretinin and calbindin immunoreactive neurons. No changes in choline acetyltransferase and calcitonin gene-related peptide immunoreactivity were observed. T. spiralis-induced colitis causes profound neuromuscular adaptations. The reduction in NOS neurons appears to underlie changes in motility.
Aulí
Mariona
M
Department of Cell Biology, Physiology and Immunology, Veterinary Faculty, Universitat Autònoma de Barcelona, Spain.
Nasser
Yasmin
Y
Hotchkiss Brain Institute and Institute for Infection, Immunity and Inflammation, Department of Physiology and Biophysics, University of Calgary, Alberta, Canada T2N 4N1.
Ho
Winnie
W
Hotchkiss Brain Institute and Institute for Infection, Immunity and Inflammation, Department of Physiology and Biophysics, University of Calgary, Alberta, Canada T2N 4N1.
Burgueño
Joan F
JF
Department of Cell Biology, Physiology and Immunology, Veterinary Faculty, Universitat Autònoma de Barcelona, Spain; Ciberehd, Ministerio de Sanidad, Spain.
Keenan
Catherine M
CM
Hotchkiss Brain Institute and Institute for Infection, Immunity and Inflammation, Department of Physiology and Biophysics, University of Calgary, Alberta, Canada T2N 4N1.
Romero
Carolina
C
Department of Cell Biology, Physiology and Immunology, Veterinary Faculty, Universitat Autònoma de Barcelona, Spain; Ciberehd, Ministerio de Sanidad, Spain.
Sharkey
Keith A
KA
Hotchkiss Brain Institute and Institute for Infection, Immunity and Inflammation, Department of Physiology and Biophysics, University of Calgary, Alberta, Canada T2N 4N1.
Fernández
Ester
E
Department of Cell Biology, Physiology and Immunology, Veterinary Faculty, Universitat Autònoma de Barcelona, Spain; Ciberehd, Ministerio de Sanidad, Spain. Electronic address: ester.fernandez@uab.es.
D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002795:Choline O-Acetyltransferase / Q000378:metabolism; D003092:Colitis / Q000378:metabolism / Q000469:parasitology / Q000503:physiopathology*; D003106:Colon / Q000294:innervation / Q000378:metabolism / Q000503:physiopathology; D004195:Disease Models, Animal; D005769:Gastrointestinal Motility / Q000502:physiology; D007150:Immunohistochemistry; D066298:In Vitro Techniques; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000294:innervation / Q000503:physiopathology; D008297:Male; D009119:Muscle Contraction / Q000502:physiology*; D009130:Muscle, Smooth / Q000294:innervation / Q000378:metabolism / Q000503:physiopathology; D009197:Myenteric Plexus / Q000503:physiopathology; D009419:Nerve Tissue Proteins / Q000378:metabolism; D019001:Nitric Oxide Synthase / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013368:Submucous Plexus / Q000503:physiopathology; D017160:Trichinella spiralis; D014235:Trichinellosis / Q000378:metabolism / Q000469:parasitology / Q000503:physiopathology*; D015431:Weight Loss / Q000502:physiology
D009419:Nerve Tissue Proteins; D009195:Peroxidase; D019001:Nitric Oxide Synthase; D002795:Choline O-Acetyltransferase; D015740:Calcitonin Gene-Related Peptide