Developmental changes in expression and self-editing of adenosine deaminase type 2 pre-mRNA and mRNA in rat brain and cultured cortical neurons.
Adenosine deaminase-1 and -2 (ADAR-1 and -2) are double-stranded RNA-specific enzymes involved in the editing of genes including serotonin 2C receptor (5-HT2CR) mRNA and ADAR-2 pre-mRNA. We reported that the editing efficacy of 5-HT2CR mRNA altered during brain development in rats. The present study aimed to clarify if changes in the expression of ADAR genes and the editing of ADAR-2 pre-mRNA occur during development. The expression level of ADAR-1 mRNA was constant during development, whereas the expression levels of ADAR-2 mRNA and ADAR-2 pre-mRNA markedly increased during development. ADAR-2 pre-mRNA possesses six editing sites. Editing of these sites did not occur during the embryonic period; however, the number of edited sites and the editing frequency at these sites increased after birth and cultivation period. These results suggest that the increases in ADAR-2 pre-mRNA editing and mRNA expression of the enzyme may play a role in development. We also discuss the relationship between 5-HT2CR mRNA editing and the expression/RNA editing of ADAR-1 and ADAR-2 mRNA.
Division of Medicinal Pharmacology, Institute of Natural Medicine, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan.
Pham Nguyet Thi
PN
Hang
Michihisa
M
Tohda
Kinzo
K
Matsumoto
D000243:Adenosine Deaminase / Q000235:genetics / Q000378:metabolism*; D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000502:physiology*; D009474:Neurons / Q000378:metabolism*; D011247:Pregnancy; D017393:RNA Editing / Q000502:physiology*; D012322:RNA Precursors / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D016601:RNA-Binding Proteins; D051381:Rats; D017208:Rats, Wistar
D012322:RNA Precursors; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C567955:ADARB1 protein, human; D000243:Adenosine Deaminase