[Up-regulation of CD86 and cytokine expression in acute leukemia cells by Ara-C].
OBJECTIVE
To observe the effects of Ara-c on the expression of CD86 molecule on acute leukemia cells and explore the possible mechanisms.


METHODS
The expression of CD86 on U937, HL-60 and NB4 cell lines treated with or without Ara-C wa assayed by flow cytometry. The mRNA expression of CD86, NF-kappaB, IFN-gamma was examined by semiquantitative RT-PCR.


RESULTS
UP-regulation of CD86 was observed on those cells treated by Ara-c. The level of CD86 and NF-kappaB mRNA in Ara-c treated cells was significantly enhanced. IFN-gamma was detectable 72 hours after T cell activation.


CONCLUSIONS
Ara-C can enhance CD86 and NF-kappaB expression on acute leukemia cells, which may play critical role in T cell activation and differentiation.
Fan
Dong-mei
DM
Institute of Hematology & Hospital of Blood Diseases, Chinese Academic of Medical Sciences & Peking Union Medical College, Tianjin, China.
Yang
Ming
M
Jia
Hai-rong
HR
Gao
Ying-dai
YD
Wang
Jin-hong
JH
Ji
Qing
Q
Xiong
Dong-sheng
DS
Yang
Chun-zheng
CZ
D051940:B7-2 Antigen / Q000235:genetics* / Q000276:immunology; D002478:Cells, Cultured; D003561:Cytarabine / Q000494:pharmacology*; D016207:Cytokines / Q000235:genetics* / Q000276:immunology; D015973:Gene Expression Regulation, Leukemic / Q000187:drug effects; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000276:immunology; D007963:Leukocytes, Mononuclear / Q000187:drug effects / Q000276:immunology; D008213:Lymphocyte Activation; D016328:NF-kappa B / Q000235:genetics / Q000276:immunology; D020298:U937 Cells; D015854:Up-Regulation / Q000187:drug effects*
D051940:B7-2 Antigen; D016207:Cytokines; D016328:NF-kappa B; D003561:Cytarabine