Interaction between E-protein and Oct transcription factors in the function of the catfish IGH enhancer.
Transcriptional control of the immunoglobulin heavy chain (IGH) locus in the channel catfish, Ictalurus punctatus, is incompletely understood. It is, however, known that 2 variant octamer motifs and a microE5 motif in the core region of the enhancer (Emicro3') are important in driving transcription, and it has been suggested that interaction between transcription factors (Oct factors and E-proteins) bound to these sites contributes to enhancer function. In this study, the functional relationships between the microE5 motif, the proximal octamer motif, and the factors that bind them have been examined. The results of mutational analysis of these motifs showed that their interaction is important to driving transcription from the enhancer. Furthermore, the catfish Oct transcription factors were capable of a physical interaction with the catfish E-proteins. These results support a role for interaction between transcription factors bound to the octamer and microE5 motifs in the function of the Emicro3' enhancer.
Hikima
Jun-Ichi
J
Marine Biomedicine and Environmental Sciences Center, Medical University of South Carolina, Charleston, SC 29412, USA.
Lennard Richard
Mara L
ML
Wilson
Melanie R
MR
Miller
Norman W
NW
Warr
Gregory W
GW
D000818:Animals; D001483:Base Sequence; D002397:Catfishes / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004742:Enhancer Elements, Genetic / Q000235:genetics*; D029941:Fish Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D007143:Immunoglobulin Heavy Chains / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D011485:Protein Binding; D014157:Transcription Factors / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015533:Transcriptional Activation / Q000235:genetics
D029941:Fish Proteins; D007143:Immunoglobulin Heavy Chains; D014157:Transcription Factors