Cultured melanocytes: from skin biopsy to transplantation.
Restoration of cutaneous pigmentation has been achieved in stable vitiligo by autologous melanocyte transplantation. This study was aimed to develop a methodology to deliver melanocytes to vitiliginous area following their processing and culture in a centralized facility. Here we report a methodology to culture melanocytes on carrier films, transport the cells, and graft them on vitiliginous areas. The salient features of this study include: 1) development of polylactic acid (PLA) films that support melanocyte attachment, growth, and delivery; 2) establish transport conditions for skin biopsies from hospitals; 3) establish transport conditions for cultured cells from cell processing center to hospitals. Results suggest that PLA films could serve as carriers for melanocytes during transport. "Upside-down" application of the graft results in the migration of cells from the films into the dermabraded area. The transport conditions ensure cell viability for 96 h. This system could help clinicians, who do not have access to cell culture facilities, transplant cultured melanocytes in a cost-effective manner.
Tissue Engineering Group, Reliance Life Sciences Pvt. Ltd., Navi Mumbai, India. deepa_ghosh@relbio.com
Deepa
D
Ghosh
Sudheer
S
Shenoy
Pushpa
P
Kuchroo
D000328:Adult; D000818:Animals; D001706:Biopsy; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D017690:Cell Transplantation / Q000379:methods*; D002478:Cells, Cultured; D015870:Gene Expression; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008544:Melanocytes / Q000166:cytology* / Q000378:metabolism / Q000637:transplantation*; D051379:Mice; D016513:Mice, SCID; D008875:Middle Aged; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism / Q000473:pathology; D014183:Transplantation, Heterologous; D014820:Vitiligo / Q000473:pathology / Q000601:surgery*
D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)