Detection of the dihydrofolate reductase-164L mutation in Plasmodium falciparum infections from Malawi by heteroduplex tracking assay.
Standard polymerase chain reaction methods often cannot detect drug-resistance mutations in Plasmodium falciparum infections if the mutation is present in < or = 20% of the parasites. A heteroduplex tracking assay was developed that can detect dihydrofolate reductase 164-L mutations in variants representing 1% of the parasites in an individual host. Using this assay, we confirmed the presence of the mutation in P. falciparum infections in Malawi.
Juliano
Jonathan J
JJ
Division of Infectious Diseases, University of North Carolina, School of Medicine, Chapel Hill, North Carolina 27599-7300, USA. jjuliano@med.unc.edu
Trottman
Paul
P
Mwapasa
Victor
V
Meshnick
Steven R
SR
D000818:Animals; D001483:Base Sequence; D015331:Cohort Studies; D017931:DNA Primers; D005260:Female; D006801:Humans; D016778:Malaria, Falciparum / Q000201:enzymology / Q000235:genetics*; D008295:Malawi; D009154:Mutation; D009692:Nucleic Acid Heteroduplexes; D010963:Plasmodium falciparum / Q000201:enzymology; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D015597:Pregnancy Complications, Parasitic / Q000235:genetics; D012680:Sensitivity and Specificity; D013762:Tetrahydrofolate Dehydrogenase / Q000235:genetics*
D017931:DNA Primers; D009692:Nucleic Acid Heteroduplexes; D013762:Tetrahydrofolate Dehydrogenase