Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study.
An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for (R)-OXY and (S)-OXY, respectively, and 70 and 76% for (R)-DEO and (S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY.
Faculdade de Ciências Farmacâeuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
Patrícia
P
da Fonseca
Luis Alexandre Pedro
LA
de Freitas
Luis Felipe Ribeiro
LF
Pinto
Cezar Rangel
CR
Pestana
Pierina Sueli
PS
Bonato
D000818:Animals; D001711:Biotransformation; D005591:Chemical Fractionation / Q000379:methods; D008297:Male; D008333:Mandelic Acids / Q000032:analysis* / Q000493:pharmacokinetics; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
D008333:Mandelic Acids; C032681:desethyloxybutynin; C005419:oxybutynin