Label-free detection of DNA molecules on the dendron based self-assembled monolayer by electrochemical impedance spectroscopy.
We report preparation of a novel platform for effective DNA hybridization and its application to the detection of single mismatched DNA. Cone-shaped dendrimer molecules have been immobilized on the gold surface at equidistance, 3.1 nm, from each other with a probe DNA molecule attached to the top of each dendrimer so that enough space would be secured for effective hybridization. This arrangement allows each probe DNA molecule to form a natural DNA double helix upon hybridization with a target DNA molecule. The single nucleotide polymorphism at either the central or end position of the 25-mer target DNA has been shown to be effectively discriminated against on this platform from each other as well as from a complementary DNA by electrochemical impedance measurements. We also report adverse effects exerted by probe ions, Fe(CN)(6)(3-/4-), on DNA hybridization reactions. The significance of the results for the use in DNA analysis is discussed.
Park
Jin-Young
JY
Department of Chemistry and Center for Integrated Molecular Systems, Pohang University of Science and Technology, Pohang, Gyeongbuk 790-784, South Korea.
Kwon
Sung Hong
SH
Park
Joon Won
JW
Park
Su-Moon
SM
D000873:Anthracenes / Q000737:chemistry*; D003486:Cyanides / Q000737:chemistry; D004247:DNA / Q000032:analysis* / Q000737:chemistry*; D004277:DNA, Single-Stranded / Q000032:analysis / Q000737:chemistry; D017097:Electric Impedance; D004563:Electrochemistry; D015203:Reproducibility of Results; D013057:Spectrum Analysis / Q000379:methods*; D013194:Staining and Labeling; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000873:Anthracenes; D003486:Cyanides; D004277:DNA, Single-Stranded; D013438:Sulfhydryl Compounds; C501244:dendron; D004247:DNA