[Isolation of Pseudomonas aurantiaca strains capable of overproduction of phenazine antibiotics].
N-methyl-N'-nitro-N-nitrosoguanidine (NH)-induced mutagenesis with subsequent selection for resistance to toxic amino acid analogues (azaserine, m-fluoro-DL-phenylalanine, and 6-diazo-5-oxo-L-norleucine) was applied to Pseudomonas aurantiaca B-162. The resulting strains produced phenazine antibiotics three times more efficiently than the wild type strain and ten times more efficiently than the known pseudomonad strains. Overproduction of phenazine antibiotics was shown to result either from deregulation of 3-deoxi-D-arabinohepulosonate-7-phosphate synthase (DAHP synthase), the key enzyme of the aromatic pathway (removal of inhibition by phenylalanine, tyrosine, and phenazine), or overproduction of N-hexanoyl homoserine lactone, the regulatory molecule of positive control of cellular metabolism (QS system).
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D010723:3-Deoxy-7-Phosphoheptulonate Synthase / Q000378:metabolism; D015107:4-Butyrolactone / Q000031:analogs & derivatives / Q000378:metabolism; D001377:Azaserine / Q000494:pharmacology; D003980:Diazooxonorleucine / Q000494:pharmacology; D024881:Drug Resistance, Bacterial; D008769:Methylnitronitrosoguanidine; D016296:Mutagenesis; D010619:Phenazines / Q000378:metabolism*; D010649:Phenylalanine / Q000494:pharmacology; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*
D010619:Phenazines; D003980:Diazooxonorleucine; C088386:homoserine lactone; D008769:Methylnitronitrosoguanidine; D010649:Phenylalanine; D001377:Azaserine; D010723:3-Deoxy-7-Phosphoheptulonate Synthase; D015107:4-Butyrolactone