Intracellular and extracellular expression of the major inducible 70kDa heat shock protein in experimental ischemia-reperfusion injury of the spinal cord.
Inflammatory responses exacerbate ischemia-reperfusion (IR) injury of spinal cord, although understanding of mediators is incomplete. The major inducible 70kDa heat shock protein (hsp70) is induced by ischemia and extracellular hsp70 (e-hsp70) can modulate inflammatory responses, but there is no published information regarding e-hsp70 levels in the cerebrospinal fluid (CSF) or serum as part of any neurological disease state save trauma. The present work addresses this deficiency by examining e-hsp70 in serum and CSF of dogs in an experimental model of spinal cord IR injury. IR injury of spinal cord caused hind limb paraplegia within 2-3 h that was correlated to lumbosacral poliomalacia with T cell infiltrates at 3 d post-ischemia. In this context, we showed a 5.2-fold elevation of e-hsp70 in CSF that was induced by ischemia and was sustained for the following 3 d observation interval. Plasma e-hsp70 levels were unaffected by IR injury, indicating e-hsp70 release from within the central nervous system. A putative source of this e-hsp70 was ependymal cells in the ischemic penumbra, based upon elevated i-hsp70 levels detected within these cells. Results warrant further investigation of e-hsp70's potential to modulate spinal cord IR injury.
Department of Anesthesiology, College of Medicine and Public Health, The Ohio State University, USA.
Hamdy
H
Awad
Zacharias
Z
Suntres
John
J
Heijmans
Daniel
D
Smeak
Valerie
V
Bergdall-Costell
Fievos L
FL
Christofi
Cynthia
C
Magro
Michael
M
Oglesbee
D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D015870:Gene Expression / Q000502:physiology*; D005786:Gene Expression Regulation / Q000502:physiology; D016179:Granulocyte Colony-Stimulating Factor / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000097:blood* / Q000134:cerebrospinal fluid*; D007377:Interleukin-3 / Q000378:metabolism; D008054:Lipid Peroxides / Q000134:cerebrospinal fluid; D009046:Motor Neurons / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011994:Recombinant Proteins; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology; D013116:Spinal Cord / Q000473:pathology; D020760:Spinal Cord Ischemia / Q000378:metabolism* / Q000473:pathology; D013929:Thromboxane B2 / Q000378:metabolism; D013997:Time Factors
D018840:HSP70 Heat-Shock Proteins; D007377:Interleukin-3; D008054:Lipid Peroxides; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C404145:myelopoietin; D016179:Granulocyte Colony-Stimulating Factor; D013929:Thromboxane B2