Pitx3-CreER mice showing restricted Cre expression in developing ocular lens and skeletal muscles.
To generate temporally controlled inactivation or activation of interested genes in Pitx3-expressing cells, the tamoxifen-inducible form of Cre, CreER(T2), was inserted into the Pitx3 locus of a mouse BAC clone. Following a single dose of tamoxifen, Cre activity in Pitx3-CreER(T2) transgenic mice was observed in the ocular lens and skeletal muscles but not in the central nervous system at various embryonic stages. This mouse line provides a reagent for driving inducible Cre-dependent recombination in the lens and skeletal muscles during embryonic development.
Qiu
Hai-Yan
HY
Institute of Neuroscience and State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, China.
Guo
Chao
C
Cheng
Xue-Wen
XW
Huang
Ying
Y
Xiong
Zhi-Qi
ZQ
Ding
Yu-Qiang
YQ
D000818:Animals; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D003433:Crosses, Genetic; D004622:Embryo, Mammalian / Q000378:metabolism; D005697:Galactosides / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000235:genetics; D018390:Gene Targeting / Q000379:methods; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D007150:Immunohistochemistry; D007211:Indoles / Q000378:metabolism; D019426:Integrases / Q000235:genetics* / Q000378:metabolism; D007908:Lens, Crystalline / Q000196:embryology / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic; D018482:Muscle, Skeletal / Q000196:embryology / Q000378:metabolism*; D009928:Organ Specificity / Q000235:genetics; D011995:Recombination, Genetic; D013629:Tamoxifen / Q000008:administration & dosage; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D019076:Transgenes
D005697:Galactosides; D018398:Homeodomain Proteins; D007211:Indoles; D014157:Transcription Factors; C433629:homeobox protein PITX3; D013629:Tamoxifen; C045073:Cre recombinase; D019426:Integrases; C044888:5-bromo-4-chloro-3-indolyl beta-galactoside