Diversity synthesis of complex pyridines yields a probe of a neurotrophic signaling pathway.
Recognizing the value of including complex pyridines in small-molecule screening collections, we developed a previously unexplored [2 + 2 + 2]-cycloaddition of silyl-tethered diynes with nitriles. The tether provides high regioselectivity, while the solvent THF allows catalytic CpCo(CO)(2) to be used without exogenous irradiation. One of the resulting bicyclic and monocyclic (desilylated) pyridines was identified as an inhibitor of neuregulin-induced neurite outgrowth (EC(50) = 0.30 microM) in a screen that probes a pathway likely to be involved in breast cancers and schizophrenia.
Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
B Lawrence
BL
Gray
Xiang
X
Wang
W Colby
WC
Brown
Letian
L
Kuai
Stuart L
SL
Schreiber
D000818:Animals; D002384:Catalysis; D015394:Molecular Structure; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism*; D009570:Nitriles / Q000737:chemistry; D016716:PC12 Cells; D011725:Pyridines / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects*
D009570:Nitriles; D011725:Pyridines