Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells.
Satratoxin H, a mycotoxin, is thought to induce apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased reactive oxygen species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS.
Department of Physiology, Faculty of Medicine, Srinakharinwirot University, Sukhumvit, Bangkok, Thailand.
Punnee
P
Nusuetrong
Thitima
T
Pengsuparp
Duangdeun
D
Meksuriyen
Masaaki
M
Tanitsu
Haruhisa
H
Kikuchi
Michinao
M
Mizugaki
Ken-ichi
K
Shimazu
Yoshiteru
Y
Oshima
Norimichi
N
Nakahata
Makoto
M
Yoshida
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D053938:DNA Fragmentation / Q000187:drug effects; D004587:Electrophoresis, Agar Gel; D005434:Flow Cytometry; D016193:G1 Phase / Q000187:drug effects; D005978:Glutathione / Q000494:pharmacology; D007202:Indicators and Reagents; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D016716:PC12 Cells; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014255:Trichothecenes / Q000037:antagonists & inhibitors / Q000633:toxicity*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
D000975:Antioxidants; D007202:Indicators and Reagents; D017382:Reactive Oxygen Species; D017392:Thiobarbituric Acid Reactive Substances; D014255:Trichothecenes; C014184:satratoxin H; D048031:JNK Mitogen-Activated Protein Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D005978:Glutathione