Removal of phytotoxic compounds from torrefied grass fibres by plant-beneficial microorganisms.
We aimed to select microorganisms colonizing torrefied grass fibres (TGF) and simultaneously reducing the phytotoxicity which appeared after heat treatment of the fibres. Eighty-eight bacterial strains and one fungus, previously isolated from a sequential enrichment experiment on torrefied fibres and extracts, were tested separately for their capacity to decrease phytotoxicity. Eleven of the bacterial strains and the fungus significantly reduced phytotoxicity. These organisms were checked for their ability to grow on agar containing phenol, 2-methoxyphenol, 2,6-dimethoxyphenol, 2-furalaldehyde, pyrrole-2-carboxaldehyde and furan-2-methanol as sole carbon sources. The fungus F/TGF15 and the bacterial strain 66/TGF15 were able to grow on all six compounds. Strains 15/TGE5, 23/TGE5, 43/TGE20, 56/TGF10 and 95/TGF15 grew on two to four compounds, and strain 72/TGF15 only on one compound. Strains 31/TGE5, 34/TGE5, 48/TGE20 and 70/TGF15 did not grow on any of the single toxic compounds. GC analyses of torrefied grass extracts (TGE) determined which compounds were removed by the microorganisms. F/TGF15 was the only isolate depleting phenol, 2-methoxyphenol, 2-dihydrofuranone and pyrrole-2,5-dione-3-ethyl-4-methyl. Strains 15/TGE5, 23/TGE5, 31/TGE5 and 56/TGF10, and the fungus depleted 2-furalaldehyde, 2-furan-carboxaldehyde-5-methyl, pyrrole-2-carboxaldehyde, 5-acetoxymethyl-2-furaldehyde and benzaldehyde-3-hydroxy-4-methoxy. These promising candidates for colonizing and simultaneously reducing the phytotoxicity of TGF were affiliated with Pseudomonas putida, Serratia plymuthica, Pseudomonas corrugata, Methylobacterium radiotolerans and Coniochaeta ligniaria.
Trifonova
Radoslava
R
Plant Research International, Wageningen, the Netherlands. radoslava.trifonova@wur.nl
Postma
Joeke
J
Verstappen
Francel W A
FW
Bouwmeester
Harro J
HJ
Ketelaars
Jan J M H
JJ
van Elsas
Jan-Dirk
JD
D000704:Analysis of Variance; D001419:Bacteria / Q000302:isolation & purification / Q000378:metabolism*; D001673:Biodegradation, Environmental; D005658:Fungi / Q000302:isolation & purification / Q000378:metabolism*; D005662:Furaldehyde / Q000378:metabolism / Q000633:toxicity; D005663:Furans / Q000378:metabolism / Q000633:toxicity; D008401:Gas Chromatography-Mass Spectrometry; D018545:Lactuca / Q000187:drug effects; D010636:Phenols / Q000378:metabolism / Q000633:toxicity; D006109:Poaceae / Q000737:chemistry / Q000382:microbiology*; D012988:Soil Microbiology
D005663:Furans; D010636:Phenols; D005662:Furaldehyde