Adsorption of Cry1Ab protein isolated from Bt transgenic rice on bentone, kaolin, humic acids, and soils.
Adsorption on a soil matrix of the insecticidal protein from Bacillus thuringiensis ( Bt) transgenic plants affects their accumulation and release and, hence, bioavailability in soil. Cry1Ab protein isolated from Bt transgenic rice was used to evaluate the adsorption and desorption on bentone, kaolin, and humic acids (HAs). The adsorption equilibrium of Cry1Ab protein was reached within 1-2 h for bentone and kaolin and within 4-8 h for HAs. The adsorption isotherms were better described by linear expressions ( R (2) >/= 0.973) rather than by the Freundlich model. No saturation was observed, even at the maximum concentration used (3.71 microg mL (-1)). The adsorbed protein was not easily desorbed at the used protein concentrations (0.18-3.71 microg mL (-1)); more than 50-70%, 70-80%, and 90% of the adsorbed protein remained on HAs, kaolin, and bentone, respectively, after washing with water. Adsorption and desorption of the Cry1Ab protein were further studied using five soils, and the isotherms were also well-described by linear equations ( p < 0.05). Adsorption of the Cry1Ab protein on soils was positively related to the soil organic matter content.
Institute of Nuclear Agricultural Sciences, Zhejiang University, Huajiachi Campus, Hangzhou 310029, China.
Haiyan
H
Wang
Qingfu
Q
Ye
Jay
J
Gan
Jianmin
J
Wu
D000327:Adsorption; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry* / Q000302:isolation & purification; D001546:Bentonite / Q000737:chemistry; D055598:Chemical Phenomena; D002627:Chemistry, Physical; D004731:Endotoxins / Q000737:chemistry* / Q000302:isolation & purification; D006460:Hemolysin Proteins / Q000737:chemistry* / Q000302:isolation & purification; D006812:Humic Substances; D007616:Kaolin / Q000737:chemistry; D007700:Kinetics; D012275:Oryza / Q000737:chemistry*; D030821:Plants, Genetically Modified / Q000737:chemistry*; D012987:Soil
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D006812:Humic Substances; D012987:Soil; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D001546:Bentonite; D007616:Kaolin