Immunoadjuvant activity of crude lectin extracted from Momordica charantia seed.
The aim of this study is to investigate the immunoadjuvant activity of the crude Momordica charantia lectin (crMCL) extracted from seed using beta-galactosidase (beta-gal) as the model antigen. BALB/c mice were injected intramuscularly with beta-gal alone or beta-gal + crMCL for up to four immunizations at two-week intervals. After administration of 2 doses, the IgG-specific titer to beta-gal was significantly higher in mice in the beta-gal + crMCL group than in that from the animals from the beta-gal alone group, while it was about the same in both groups after 1 dose. Our data suggest that crMCL may help raise antibodies under the prime and boost administration regimen and could be a potent vaccine adjuvant.
Huang
Li
L
Department of Veterinary Microbiology, University of Miyazaki, Miyazaki, Japan.
Ikejiri
Ai
A
Shimizu
Yuya
Y
Adachi
Takumi
T
Goto
Yoshitaka
Y
Toyama
Jun
J
Tanaka
Hidenori
H
Akashi
Ryo
R
Uchida
Kazuyuki
K
Miyata
Hironori
H
Haga
Takeshi
T
D000276:Adjuvants, Immunologic / Q000737:chemistry* / Q000494:pharmacology*; D000818:Animals; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D037102:Lectins / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D029775:Momordica / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D001616:beta-Galactosidase / Q000276:immunology*
D000276:Adjuvants, Immunologic; D007074:Immunoglobulin G; D037102:Lectins; D001616:beta-Galactosidase