Aberrant gene expression in deceased transgenic cloned calves.
Several transgenic cloned species have been obtained; however, the efficiency of transgenic cloning remains very low, even lower than cloning. Many experiments have demonstrated abnormal growth and development, and inappropriate gene expression in cloned animals. In this study, we examined the expression of 19 development-related genes in lungs of three normal controls and three aberrant transgenic cloned calves. Results showed in transgenic cloned calves, 84.2% genes had decreased expression levels, however, 5.3% genes had increased levels. This study suggests transgenic cloning and the aberrant expression would cause abnormal growth and development in transgenic cloned calves. To our knowledge, this is the first time that gene expression was examined in transgenic cloned cattle. These findings may have some implications in understanding the low efficiency of the transgenic cloning.
Zhang
L
L
China Agricultural University, Haidian District, Beijing, PR China. red3stones@vip.sina.com
Wang
S H
SH
Dai
Y P
YP
Li
N
N
D000818:Animals; D030801:Animals, Genetically Modified / Q000235:genetics* / Q000254:growth & development*; D002417:Cattle / Q000235:genetics*; D019976:Cloning, Organism / Q000009:adverse effects* / Q000379:methods / Q000401:mortality; D020964:Embryo Loss / Q000235:genetics; D015870:Gene Expression; D006128:Growth / Q000235:genetics; D008168:Lung / Q000737:chemistry; D053652:Nuclear Transfer Techniques / Q000401:mortality / Q000662:veterinary; D016133:Polymerase Chain Reaction; D014162:Transfection / Q000379:methods / Q000662:veterinary