RNA editing regulates insect gamma-aminobutyric acid receptor function and insecticide sensitivity.
A-to-I pre-mRNA editing by adenosine deaminase enzymes has been reported to enhance protein diversity in the nervous system. In Drosophila, the resistance to dieldrin (RDL) gamma-aminobutyric acid (GABA) receptor subunit displays an editing site (R122) that is close to the putative GABA-binding site. We assessed the functional effects of editing at this site by expressing homomeric RDL receptors in Xenopus oocytes. After replacement of arginine 122 with a glycine, both agonist and fipronil potencies were shifted to the right in either fipronil-sensitive receptors or mutated resistant receptors (A301G/T350M). These data provide the first insight on the influence of RNA editing on GABA receptor function.
IFR QUASAV 149, RCIM Laboratory, UPRES-EA 2647/USC INRA, University of Angers, UFR Sciences, 2 Boulevard Lavoisier, F-49045 Angers Cedex, France.
Zeineb
Z
Es-Salah
Bruno
B
Lapied
Gaëlle
G
Le Goff
Alain
A
Hamon
D000818:Animals; D001120:Arginine / Q000235:genetics; D004305:Dose-Response Relationship, Drug; D004330:Drosophila; D029721:Drosophila Proteins; D015870:Gene Expression / Q000187:drug effects*; D019476:Insect Proteins / Q000502:physiology*; D007306:Insecticides / Q000494:pharmacology*; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D008958:Models, Molecular; D009154:Mutation / Q000502:physiology; D009865:Oocytes; D018408:Patch-Clamp Techniques; D011720:Pyrazoles / Q000494:pharmacology*; D017393:RNA Editing / Q000502:physiology*; D018079:Receptors, GABA / Q000235:genetics / Q000502:physiology*; D014982:Xenopus laevis; D005680:gamma-Aminobutyric Acid / Q000494:pharmacology
D029721:Drosophila Proteins; D019476:Insect Proteins; D007306:Insecticides; D011720:Pyrazoles; D018079:Receptors, GABA; D005680:gamma-Aminobutyric Acid; D001120:Arginine; C082360:fipronil