Silencing of tripartite motif protein (TRIM) 5alpha mediated anti-HIV-1 activity by truncated mutant of TRIM5alpha.
Tripartite motif protein (TRIM) 5alpha is a restriction factor of human immunodeficiency virus type 1 in Old World monkey cell. It was found that both naturally occurring and artificial TRIM5alpha variants lacking the SPRY domain could silence TRIM5alpha activity. Specifically, the artificial TRIM5alpha mutant could suppress TRIM5alpha activity of various primate species with even higher efficiency than could small interfering RNAs. The findings indicate that TRIM5alpha variants lacking the SPRY domain are useful for silencing TRIM5alpha activity.
Department of Viral Infections, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita-shi, Osaka 565-0871, Japan.
Hikoichiro
H
Maegawa
Emi E
EE
Nakayama
Ayumu
A
Kuroishi
Tatsuo
T
Shioda
D000595:Amino Acid Sequence; D000818:Animals; D019380:Anti-HIV Agents / Q000494:pharmacology*; D000090103:Antiviral Restriction Factors; D001483:Base Sequence; D015153:Blotting, Western; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D002522:Chlorocebus aethiops; D017931:DNA Primers; D020868:Gene Silencing; D005822:Genetic Vectors; D015497:HIV-1 / Q000187:drug effects*; D000882:Haplorhini; D006801:Humans; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009154:Mutation; D017384:Sequence Deletion; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases
D019380:Anti-HIV Agents; D000090103:Antiviral Restriction Factors; D002352:Carrier Proteins; D017931:DNA Primers; D000070584:Tripartite Motif Proteins; C477346:TRIM5 protein, human; D044767:Ubiquitin-Protein Ligases