Pichindé virus is trafficked through a dynamin 2 endocytic pathway that is dependent on cellular Rab5- and Rab7-mediated endosomes.
Pichindé virus (PICV) is a New World arenavirus that has been shown to enter cells through a clathrin-dependent endocytic pathway. In this study, we determined that PICV is trafficked through the cellular dynamin 2 (dyn2) endocytic pathway. Additionally, the data suggest that PICV entry is pH-dependent and that the virus travels through Rab5-mediated early and Rab7-mediated late endosomes. In all, this study characterizes the endocytic pathway utilized by the arenavirus PICV.
Department of Pathology, Institute for Human Infections and Immunity, The University of Texas Medical Branch, Galveston, TX, USA. velae@battelle.org
Eric M
EM
Vela
Tonya M
TM
Colpitts
Lihong
L
Zhang
Robert A
RA
Davey
Judith F
JF
Aronson
D000818:Animals; D018050:Arenavirus / Q000502:physiology*; D002522:Chlorocebus aethiops; D034285:Dynamin II / Q000378:metabolism*; D011992:Endosomes / Q000737:chemistry / Q000821:virology*; D006863:Hydrogen-Ion Concentration; D014709:Vero Cells; D053586:Virus Internalization; D020691:rab GTP-Binding Proteins / Q000032:analysis; D020696:rab5 GTP-Binding Proteins / Q000032:analysis; D000090802:rab7 GTP-Binding Proteins
D000090802:rab7 GTP-Binding Proteins; C000716463:rab7 GTP-binding proteins, human; D020691:rab GTP-Binding Proteins; D020696:rab5 GTP-Binding Proteins; D034285:Dynamin II