Positive correlation of cell-free DNA in plasma/serum in patients with malignant and benign breast disease.
BACKGROUND
Circulating cell-free (ccf) DNA is measurable in healthy individuals and in higher concentration in patients with benign and malignant breast disease (BD).


METHODS
In paired plasma and serum samples ccf DNA was extracted and quantified by real-time quantitative PCR for the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene.


RESULTS
The concentration of ccf DNA in serum was higher in patients with benign and malignant BD (p = 0.023/p = 0.001) compared to healthy controls, whereas ccf DNA in plasma was higher in patients with malignant BD compared to patients with benign BD or healthy controls (p = 0.012/0.007). The ccf DNA correlated significantly between plasma and serum samples in patients with benign (p = 0.01; R: 0.677) as well as malignant BD (p = 0.01; R:0.713).


CONCLUSIONS
The positive correlation between ccf DNA in plasma and serum in patients with benign as well as malignant BD, might have a diagnostic value for discriminating between malignant and benign BD.
Department of Obstetrics and Gynecology, University Hospital, Basel, Switzerland.
Rosanna
R
Zanetti-Dällenbach
Edward
E
Wight
Alex Xiu-Cheng
AX
Fan
Olav
O
Lapaire
Sinuhe
S
Hahn
Wolfgang
W
Holzgreve
Xiao Yan
XY
Zhong
D000328:Adult; D000368:Aged; D001941:Breast Diseases / Q000097:blood* / Q000235:genetics; D001943:Breast Neoplasms / Q000097:blood* / Q000235:genetics*; D004247:DNA / Q000097:blood*; D005260:Female; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics; D006801:Humans; D010949:Plasma; D044967:Serum
D004247:DNA; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases