The carcinoGENOMICS project: critical selection of model compounds for the development of omics-based in vitro carcinogenicity screening assays.
Recent changes in the European legislation of chemical-related substances have forced the scientific community to speed up the search for alternative methods that could partly or fully replace animal experimentation. The Sixth Framework Program project carcinoGENOMICS was specifically raised to develop omics-based in vitro screens for testing the carcinogenic potential of chemical compounds in a pan-European context. This paper provides an in-depth analysis of the complexity of choosing suitable reference compounds used for creating and fine-tuning the in vitro carcinogenicity assays. First, a number of solid criteria for the selection of the model compounds are defined. Secondly, the strategy followed, including resources consulted, is described and the selected compounds are briefly illustrated. Finally, limitations and problems encountered during the selection procedure are discussed. Since selecting an appropriate set of chemicals is a frequent impediment in the early stages of similar research projects, the information provided in this paper might be extremely valuable.
Vinken
Mathieu
M
Department of Toxicology, Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090 Brussels, Belgium. mvinken@vub.ac.be
Doktorova
Tatyana
T
Ellinger-Ziegelbauer
Heidrun
H
Ahr
Hans-Jürgen
HJ
Lock
Edward
E
Carmichael
Paul
P
Roggen
Erwin
E
van Delft
Joost
J
Kleinjans
Jos
J
Castell
José
J
Bort
Roque
R
Donato
Teresa
T
Ryan
Michael
M
Corvi
Raffaella
R
Keun
Hector
H
Ebbels
Timothy
T
Athersuch
Toby
T
Sansone
Susanna-Assunta
SA
Rocca-Serra
Philippe
P
Stierum
Rob
R
Jennings
Paul
P
Pfaller
Walter
W
Gmuender
Hans
H
Vanhaecke
Tamara
T
Rogiers
Vera
V
D000826:Animal Testing Alternatives; D015197:Carcinogenicity Tests / Q000379:methods*; D005062:European Union; D020869:Gene Expression Profiling; D023281:Genomics / Q000379:methods*; D015386:Hazardous Substances; D007391:International Cooperation; D043922:Toxicogenetics / Q000639:trends
D015386:Hazardous Substances