In vitro evaluation of the effect of nicotine, cotinine, and caffeine on oral microorganisms.
The aim of this in vitro study was to evaluate the effects of nicotine, cotinine, and caffeine on the viability of some oral bacterial species. It also evaluated the ability of these bacteria to metabolize those substances. Single-species biofilms of Streptococcus gordonii, Porphyromonas gingivalis, or Fusobacterium nucleatum and dual-species biofilms of S. gordonii -- F. nucleatum and F. nucleatum -- P. gingivalis were grown on hydroxyapatite discs. Seven species were studied as planktonic cells, including Streptococcus oralis, Streptococcus mitis, Propionibacterium acnes, Actinomyces naeslundii, and the species mentioned above. The viability of planktonic cells and biofilms was analyzed by susceptibility tests and time-kill assays, respectively, against different concentrations of nicotine, cotinine, and caffeine. High-performance liquid chromatography was performed to quantify nicotine, cotinine, and caffeine concentrations in the culture media after the assays. Susceptibility tests and viability assays showed that nicotine, cotinine, and caffeine cannot reduce or stimulate bacterial growth. High-performance liquid chromatography results showed that nicotine, cotinine, and caffeine concentrations were not altered after bacteria exposure. These findings indicate that nicotine, cotinine, and caffeine, in the concentrations used, cannot affect significantly the growth of these oral bacterial strains. Moreover, these species do not seem to metabolize these substances.
Cogo
Karina
K
Department of Physiological Sciences, Area of Pharmacology, Anesthesiology and Therapeutics, Dentistry School of Piracicaba, State University of Campinas (UNICAMP), Piracicaba, SP, Brazil. ka.cogo@fop.unicamp.br
Montan
Michelle Franz
MF
Bergamaschi
Cristiane de Cássia
Cde C
D Andrade
Eduardo
E
Rosalen
Pedro Luiz
PL
Groppo
Francisco Carlos
FC
D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D002110:Caffeine / Q000378:metabolism / Q000494:pharmacology*; D003367:Cotinine / Q000378:metabolism / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D050296:Microbial Viability / Q000187:drug effects; D009055:Mouth / Q000382:microbiology*; D009538:Nicotine / Q000378:metabolism / Q000494:pharmacology*; D010510:Periodontal Diseases / Q000382:microbiology*
D002110:Caffeine; D009538:Nicotine; D003367:Cotinine