[Role of aquaporin 1 in the migration of eosinophils from asthmatic guinea pigs].
OBJECTIVE
To investigate the role of aquaporin 1 (AQP1) in the migration of eosinophils (EOS) and to determine if AQP-1 can be viewed as the chemotactic activity marker of EOS.


METHODS
Asthma model of guinea pigs were developed and EOS were purified from both peripheral blood and bronchoalveolar lavage fluid (BALF). The smears of EOS were studied by in situ hybridization for determining AQP1 mRNA and immunofluorescence under laser scanning confocal microscope for determining AQP1 protein.


RESULTS
AQP1 was found expressed in EOS both from peripheral blood and from BALF. Compared with the expression of AQP1 mRNA (mean grey value 109.200 +/- 5.756, x +/- s) and protein (average fluorescence intensity 279.926 +/- 11.293) in EOS from BALF, there was stronger expression of AQP1 mRNA (92.904 +/- 3.290) and protein (425.081 +/- 17.474) in EOS from peripheral blood. The difference both of AQP1 mRNA (t = 9.519, P < 0.05) and protein(t = 27.020, P < 0.05) were considered statistically significant respectively.


CONCLUSIONS
AQP1 plays a crucial role in EOS movement. It is possible that EOS produce more AQP1 protein to accelerate its migration to inflammatory tissues under allergic disease and EOS with AQP1 highly expressed are activated. AQP1 can be viewed as the chemotactic activity marker of EOS.
Lei
Fei
F
Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
Zhu
Dong-Dong
DD
Wang
Xin-Rui
XR
Guan
Gui-Mei
GM
Jiang
Yun-Peng
YP
Dong
Zhen
Z
D000818:Animals; D051398:Aquaporin 1 / Q000378:metabolism*; D001249:Asthma / Q000378:metabolism*; D001992:Bronchoalveolar Lavage Fluid; D004804:Eosinophils / Q000378:metabolism*; D006168:Guinea Pigs; D008297:Male; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D051398:Aquaporin 1