Effects of Sertoli cell-conditioned medium on ventral midbrain neural stem cells: a preliminary report.
The 796RMB cell line is a multipotent stem cell line isolated from human fetal midbrain tissues, a region from which dopamine neurons of the substantia nigra develop. It would be useful to increase the dopaminergic characteristics of this cell line to enhance its usefulness as a cell therapy for Parkinson's disease utilizing transplantation protocols. Sertoli cells and its conditioned media isolated from the testis have been previously shown to enhance tyrosine hydroxylase expression in ventral mesencephalon neurons both in vitro and in vivo. Therefore, the present preliminary study investigated the ability of Sertoli cell pre-conditioned medium to enhance differentiation of the 796MB cell line toward the domaminergic phenotype. Results showed that secretory products derived from Sertoli cell conditioned medium increased cell proliferation and enhanced dopaminergic neuronal differentiation of the 796RMB cell line. These findings may lead to alternative therapeutic cell transplantation protocols for the treatment of Parkinson's disease.
Shamekh
Rania
R
Center of Excellence for Aging and Brain Repair, Department of Neurosurgery, University of South Florida College of Medicine, Tampa, FL 33612, USA.
Saporta
Samuel
S
Cameron
Don F
DF
Willing
Alison E
AE
Sanberg
Cindy D
CD
Johe
Karl
K
Sanberg
P R
PR
D000818:Animals; D000831:Animals, Newborn; D002452:Cell Count / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D017077:Culture Media, Conditioned / Q000494:pharmacology*; D004298:Dopamine / Q000378:metabolism; D005333:Fetus; D006801:Humans; D008297:Male; D008636:Mesencephalon / Q000166:cytology*; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009474:Neurons / Q000502:physiology*; D051381:Rats; D012708:Sertoli Cells / Q000737:chemistry*; D013234:Stem Cells / Q000187:drug effects*; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D017077:Culture Media, Conditioned; C489788:MAP2 protein, human; D008869:Microtubule-Associated Proteins; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine