Ruminococcus gauvreauii sp. nov., a glycopeptide-resistant species isolated from a human faecal specimen.
A novel strictly anaerobic, vancomycin-resistant, Gram-positive coccus (strain CCRI-16,110(T)) was isolated from a human faecal specimen. This strain was characterized using morphological, biochemical and molecular taxonomic methods. The organism was unable to hydrolyse aesculin and failed to produce acid from cellobiose, d-lactose and alpha-raffinose. Acetic acid was the sole product of glucose fermentation by the organism. On the basis of 16S rRNA and tuf gene sequence comparison, strain CCRI-16,110(T) was most closely related to species of the genus Ruminococcus and formed a hitherto unknown sublineage within the Clostridium coccoides rRNA cluster of organisms (cluster XIVa). Based on phenotypic and phylogenetic evidence, a novel species, Ruminococcus gauvreauii sp. nov., is proposed. The type strain is CCRI-16,110(T) (=NML 060141(T) =CCUG 54,292(T) =JCM 14987(T)).
Domingo
M-C
MC
Centre de Recherche en Infectiologie de l'Université Laval, CHUQ, Pavillon CHUL, 2705 boul. Laurier, Québec, QC, G1V 4G2, Canada.
Huletsky
A
A
Boissinot
M
M
Bernard
K A
KA
Picard
F J
FJ
Bergeron
M G
MG
D000693:Anaerobiosis; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005243:Feces / Q000382:microbiology*; D020459:Genes, rRNA; D006020:Glycopeptides / Q000494:pharmacology; D006801:Humans; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045857:Ruminococcus / Q000145:classification* / Q000187:drug effects* / Q000302:isolation & purification / Q000502:physiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D020713:Vancomycin Resistance
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D006020:Glycopeptides; D012336:RNA, Ribosomal, 16S