Associations of seroreactivity against crystallin proteins with disease activity and cataract in patients with uveitis.
OBJECTIVE
betaB1-crystallin is a putative target of an autoantibody observed in a subset of patients with uveitis. The purpose of this study was to determine whether seroreactivity against betaB1 or other specific purified crystallin proteins is observed in patients with uveitis and whether this reactivity is associated with either cataract or active intraocular inflammation.


METHODS
Sera from patients with uveitis were tested for IgG antibodies with reactivity against alphaA-, alphaB-, betaB1-, or betaB2-crystallin proteins using a modified slot-blot protocol. Ophthalmic evaluations included analysis of the degree of intraocular inflammation and assessment of lens opacity by the Lens Opacities Classification System (LOCS) III. Positive anti-crystallin reactivity was defined as greater than the mean + 2 SD of the reactivity of a commercially available control serum panel. Statistical analysis was performed with the Fisher exact test, Kruskal-Wallis test, and Student's t-test.


RESULTS
IgG antibodies against alphaA-, alphaB-, or betaB1-crystallin were identified in 70% of 39 subjects; in contrast, only 30% of the control sera exhibited reactivity against one or more of these crystallin proteins (P <or= 0.01). Seroreactivity against alphaA-, alphaB-, or betaB1-, but not betaB2-crystallin was related to active anterior segment inflammation. Seroreactivity against alphaB and betaB1 was significantly related to cortical cataract (P <or= 0.05).


CONCLUSIONS
Serum antibodies against specific crystallin proteins are present in most patients with uveitis. The relationship between the presence of specific anti-crystallin antibodies and active inflammation may indicate a role for these autoantibodies in uveitis pathogenesis.
Departments of 2Ophthalmology, Ocular Inflammatory Disease Center, Jules Stein Eye Institute, University of California, Los Angeles, California 90095-7000, USA.
Ling
L
Chen
Gary N
GN
Holland
Fei
F
Yu
Ralph D
RD
Levinson
Kirsten J
KJ
Lampi
Joseph
J
Horwitz
Lynn K
LK
Gordon
D000293:Adolescent; D000328:Adult; D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D001324:Autoantigens / Q000276:immunology*; D002386:Cataract / Q000145:classification / Q000276:immunology*; D003459:Crystallins / Q000276:immunology*; D005260:Female; D006801:Humans; D015151:Immunoblotting; D007074:Immunoglobulin G / Q000032:analysis; D008297:Male; D008875:Middle Aged; D014606:Uveitis, Anterior / Q000276:immunology*; D038202:alpha-Crystallin A Chain / Q000276:immunology; D038203:alpha-Crystallin B Chain / Q000276:immunology; D038206:beta-Crystallin B Chain / Q000276:immunology
D001323:Autoantibodies; D001324:Autoantigens; C105601:CRYBB1 protein, human; D003459:Crystallins; D007074:Immunoglobulin G; D038202:alpha-Crystallin A Chain; D038203:alpha-Crystallin B Chain; D038206:beta-Crystallin B Chain; C113634:beta-crystallin B2