Detection of pancreatic cancer using antibody microarray-based serum protein profiling.
The driving force behind oncoproteomics is to identify protein signatures that are associated with a particular malignancy. Here, we have used a recombinant scFv antibody microarray in an attempt to classify sera derived from pancreatic adenocarcinoma patients versus healthy subjects. Based on analysis of nonfractionated, directly labeled, whole human serum proteomes we have identified a protein signature based on 19 nonredundant analytes, that discriminates between cancer patients and healthy subjects. Furthermore, a potential protein signature, consisting of 21 protein analytes, could be defined that was shown to be associated with cancer patients having a life expectancy of <12 months. Taken together, the data suggest that antibody microarray analysis of complex proteomes will be a useful tool to define disease associated protein signatures.
Department of Immunotechnology, Lund University, Lund, Sweden.
Johan
J
Ingvarsson
Christer
C
Wingren
Anders
A
Carlsson
Peter
P
Ellmark
Britta
B
Wahren
Gunnel
G
Engström
Ulrika
U
Harmenberg
Morten
M
Krogh
Carsten
C
Peterson
Carl A K
CA
Borrebaeck
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000912:Antibodies, Neoplasm / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism; D001798:Blood Proteins / Q000737:chemistry*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007128:Immunoglobulin Fragments / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000737:chemistry; D010190:Pancreatic Neoplasms / Q000097:blood* / Q000175:diagnosis* / Q000378:metabolism; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics / Q000379:methods
D000912:Antibodies, Neoplasm; D014408:Biomarkers, Tumor; D001798:Blood Proteins; D007128:Immunoglobulin Fragments; D009363:Neoplasm Proteins