Topical anti-inflammatory activity of Serjania erecta Radlk (Sapindaceae) extracts.
Serjania erecta Radlk (Sapindaceae), commonly called cinco-folhas or cipó-cinco-folhas in Brazil, is thought to be effective for treating several inflammatory diseases. In order to verify the topical anti-inflammatory effect of Serjania erecta, hydroalcoholic extract and fractions were obtained by extraction in solvents of increasing polarity and were tested in mouse models using croton-oil-induced inflammation. Our findings showed that topical application of Serjania erecta hydroalcoholic extract (0.01-3.0 mg/ear), and the dichloromethane (0.03-1.0 mg/ear), ethyl acetate (0.03-1.0 mg/ear), and hexane (0.003-1.0 mg/ear) fractions revealed significant activity, causing a dose-dependent reduction of croton-oil ear edema (ID(50)=0.14 mg/ear, 0.23 mg/ear, 0.14 mg/ear, 0.04 mg/ear, respectively). The extract and all tested fractions also decreased tissue myeloperoxidase activity (indicative of polymorphonuclear leukocytes influx) in mouse-ears treated with croton oil with a maximum inhibition of 72% at 3.0 mg/ear for the hydroalcoholic extract and 81%, 78%, and 83% at 1.0mg/ear for dichloromethane, ethyl acetate and hexane fractions, respectively. As expected, dexamethasone (0.05 mg/ear) was effective in inhibiting both edema and myeloperoxidase activity (99% and 82%, respectively). In conclusion, our results indicate a topical anti-inflammatory effect for the species of Serjania studied.
Gomig
Franciane
F
Departamento de Farmacologia, Universidade Federal do Paraná, Centro Politécnico, Curitiba, PR, Brazil.
Pietrovski
Evelise Fernandes
EF
Guedes
Alessandro
A
Dalmarco
Eduardo Monguilhott
EM
Calderari
Mirele Titton
MT
Guimarães
Claudio Laurentino
CL
Pinheiro
Rafael Mota
RM
Cabrini
Daniela Almeida
DA
Otuki
Michel Fleith
MF
D000279:Administration, Cutaneous; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001938:Brazil; D003436:Croton Oil; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004487:Edema / Q000188:drug therapy; D007249:Inflammation / Q000188:drug therapy*; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D029631:Sapindaceae / Q000737:chemistry*; D012871:Skin Diseases / Q000188:drug therapy; D012997:Solvents / Q000737:chemistry
D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D012997:Solvents; D003436:Croton Oil; D009195:Peroxidase