Injectable biodegradable polyurethane scaffolds with release of platelet-derived growth factor for tissue repair and regeneration.
OBJECTIVE
The purpose of this work was to investigate the effects of triisocyanate composition on the biological and mechanical properties of biodegradable, injectable polyurethane scaffolds for bone and soft tissue engineering.


METHODS
Scaffolds were synthesized using reactive liquid molding techniques, and were characterized in vivo in a rat subcutaneous model. Porosity, dynamic mechanical properties, degradation rate, and release of growth factors were also measured.


RESULTS
Polyurethane scaffolds were elastomers with tunable damping properties and degradation rates, and they supported cellular infiltration and generation of new tissue. The scaffolds showed a two-stage release profile of platelet-derived growth factor, characterized by a 75% burst release within the first 24 h and slower release thereafter.


CONCLUSIONS
Biodegradable polyurethanes synthesized from triisocyanates exhibited tunable and superior mechanical properties compared to materials synthesized from lysine diisocyanates. Due to their injectability, biocompatibility, tunable degradation, and potential for release of growth factors, these materials are potentially promising therapies for tissue engineering.
Department of Chemical Engineering, Vanderbilt University, Nashville, Tennessee, USA.
Andrea E
AE
Hafeman
Bing
B
Li
Toshitaka
T
Yoshii
Katarzyna
K
Zienkiewicz
Jeffrey M
JM
Davidson
Scott A
SA
Guelcher
D020341:Absorbable Implants; D000818:Animals; D001696:Biomechanical Phenomena; D001861:Bone Regeneration / Q000187:drug effects*; D002460:Cell Line; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000633:toxicity; D004337:Drug Carriers; D004548:Elasticity; D006868:Hydrolysis; D007279:Injections, Subcutaneous; D017953:Isocyanates / Q000737:chemistry* / Q000633:toxicity; D007700:Kinetics; D008297:Male; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects; D010982:Platelet-Derived Growth Factor / Q000008:administration & dosage / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D011140:Polyurethanes / Q000737:chemistry* / Q000633:toxicity; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012995:Solubility; D013696:Temperature; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D003432:Cross-Linking Reagents; D004337:Drug Carriers; D017953:Isocyanates; D010982:Platelet-Derived Growth Factor; D011140:Polyurethanes