Heart-cutting two-dimensional liquid chromatography methods for quantification of 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole in Class III caramel colours.
Heart-cutting two-dimensional liquid chromatography/UV detection methods were developed to determine 2-acetyl-4-(1,2,3,4-tetrahydroxybutyl)imidazole (noted as THI) in Class III caramel colours. In the first dimension, the caramel sample diluted in water was fractionated either by reversed-phase chromatography on C18 silica packing or by ion-exchange chromatography using strong cationic exchanger (SCX) stationary phase. In each case, THI elution domain was recovered in a loop and transferred to a second column packed with porous graphitic carbon (PGC) for the selective separation of THI. From the accuracy profiles with acceptance limit fixed at 20% and beta-expectation tolerance interval fixed at 90%, the two methods, C18-PGC and SCX-PGC, were validated for THI/caramel ratios included between 5-50 and 10-50 ppm, respectively. Finally, the two methods were compared for the analysis of numerous caramel samples.
Moretton
Cédric
C
Laboratoire des Sciences Analytiques, UMR CNRS 5180, Université Claude Bernard Lyon 1, Université de Lyon, Bâtiment CPE, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France. cedric.moretton@nigay.com
Crétier
Gérard
G
Nigay
Henri
H
Rocca
Jean-Louis
JL
D002853:Chromatography, Liquid / Q000295:instrumentation* / Q000379:methods*; D005505:Food Coloring Agents / Q000737:chemistry*; D007093:Imidazoles / Q000032:analysis*; D015203:Reproducibility of Results
D005505:Food Coloring Agents; D007093:Imidazoles; C049929:2-acetyl-4(5)-tetrahydroxybutylimidazole