Label-free sugar detection using phenylboronic acid-functionalized piezoresistive microcantilevers.
By appropriate surface functionalization using a thiolated phenylboronic acid derivative, gold-coated piezoresistive microcantilevers responsive to analytes having vicinal cis-diols were fashioned. A reference, uncoated cantilever permitted reliable subtraction of background signal (i.e., differential measurement). Experiments carried out in static mode under flow conditions, using the monosaccharide D-(-)-fructose as a test analyte in aqueous buffer, showed that the measured stationary-state surface stress was linear in analyte concentration over the 0-25 mM range, allowing this sugar to be readily detected at physiologically meaningful concentrations and at neutral pH. This approach offers a simple, reagentless, and flexible format for polyol screening that does not require aqueous alkaline solutions, suggesting potential applications in biomass process monitoring and glucose assay.
Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
Gary A
GA
Baker
Ramya
R
Desikan
Thomas
T
Thundat
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D001897:Boronic Acids / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D005632:Fructose / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011955:Receptors, Drug / Q000737:chemistry*; D017655:Silicon Compounds / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
C510906:4-mercaptophenylboronic acid; D001897:Boronic Acids; D011955:Receptors, Drug; D017655:Silicon Compounds; D013438:Sulfhydryl Compounds; D005632:Fructose; C032734:silicon nitride