Isolation and characterization of ZH14 with antiviral activity against Tobacco mosaic virus.
A large number of bacteria were isolated from plant samples and screened for antiviral activity against the Tobacco mosaic virus (TMV). The bacterium ZH14, which was isolated from Chinese Anxi oolong tea, secreted the antiviral substances, having 94.2% virus inhibition when the bacterial culture filtrate and TMV extract were mixed at a ratio of 1:1. The ZH14 strain is a gram-positive, spore-forming rod and has the ability to degrade ribonucleic acid. Based on its effectiveness on virus inhibition, ZH14 was selected for characterization and was identified as a strain of the Bacillus cereus group based on phenotypic tests and comparative analysis of its 16S rDNA sequence. At the same time, we determined the antiviral product of ZH14 as an extracellular protein with high molecular mass, having an optimum temperature of 15-60 degrees C and an optimum pH of 6-10. Hence, the ZH14 strain and its culture filtrate have potential application in controlling plant diseases caused by TMV.
Zhou
Wen-Wen
WW
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China. zww2003@gmail.com
Zhang
Li-Xiang
LX
Zhang
Bin
B
Wang
Fei
F
Liang
Zhi-Hong
ZH
Niu
Tian-Gui
TG
D000998:Antiviral Agents / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001409:Bacillus cereus / Q000737:chemistry* / Q000145:classification / Q000302:isolation & purification* / Q000502:physiology; D001426:Bacterial Proteins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D028241:Camellia sinensis / Q000382:microbiology; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D008969:Molecular Sequence Data; D010641:Phenotype; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D010944:Plants / Q000382:microbiology / Q000821:virology; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012367:RNA, Viral / Q000378:metabolism; D017422:Sequence Analysis, DNA; D013662:Tea / Q000382:microbiology; D013696:Temperature; D014027:Tobacco Mosaic Virus / Q000187:drug effects / Q000502:physiology*
D000998:Antiviral Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D012367:RNA, Viral; D013662:Tea