Sialyl lewisx antigen-expressing human CD4+ T and CD8+ T cells as initial immune responders in memory phenotype subsets.
Cytokine production by memory T cells in secondary immune responses has a critical role in host defenses. Previously, we had demonstrated that a unique antigen composed of sialyl lewis(x) (sLe(x)) was expressed on CD45RO(+) memory-phenotype subsets of human T cells. Here, we found that the sLe(x) antigen was up-regulated on CD45RA(+) naïve human CD4(+) T and CD8(+) T cells by TCR stimulation. In addition, sLe(x) antigen-expressing CD4(+) T and CD8(+) T cells in human PBMCs were activated immediately by cytokine stimulations composed of IL-2 plus IL-12 or IL-15 in an antigen-independent manner. Moreover, the sLe(x)-positive human CD8(+) T cells significantly enhanced reverse antibody-dependent cellular cytotoxicity compared with a sLe(x)-negative population. These findings clearly indicate that sLe(x) antigen-expressing memory phenotype CD4(+) T and CD8(+) T cells contribute to early-stage immunity by providing a source of IFN-gamma and cytotoxicity, suggesting that they would be a key immunomodulator in host defenses.
Zhang
Yue
Y
Division of ROYCE Health Bioscience, Institute for Genetic Medicine, Hokkaido University, Sapporo, 001-0021, Japan.
Ohkuri
Takayuki
T
Wakita
Daiko
D
Narita
Yoshinori
Y
Chamoto
Kenji
K
Kitamura
Hidemitsu
H
Nishimura
Takashi
T
D000920:Antibody-Dependent Cell Cytotoxicity; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation; D002470:Cell Survival / Q000276:immunology; D002478:Cells, Cultured; D005647:Fucosyltransferases; D006801:Humans; D007156:Immunologic Memory / Q000276:immunology*; D018664:Interleukin-12 / Q000378:metabolism; D017493:Leukocyte Common Antigens / Q000276:immunology / Q000502:physiology; D016256:Lewis X Antigen / Q000276:immunology; D009844:Oligosaccharides / Q000276:immunology*; D010641:Phenotype; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000378:metabolism; D000080506:Sialyl Lewis X Antigen
D016256:Lewis X Antigen; D009844:Oligosaccharides; D016692:Receptors, Antigen, T-Cell, gamma-delta; D000080506:Sialyl Lewis X Antigen; D018664:Interleukin-12; D005647:Fucosyltransferases; C019818:3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase; D017493:Leukocyte Common Antigens