High-performance liquid chromatography analysis methods developed for quantifying enzymatic esterification of flavonoids in ionic liquids.
Methods using reversed-phase high-performance liquid chromatography (RP-HPLC) with ELSD were investigated to quantify enzymatic reactions of flavonoids with fatty acids in the presence of diverse room temperature ionic liquids (RTILs). A buffered salt (preferably triethylamine-acetate) was found essential for separation of flavonoids from strongly polar RTILs, whereby RTILs were generally visible as two major peaks identified based on an ion-pairing/exchanging hypothesis. C8 and C12 stationary phases were optimal while mobile phase pH (3-7) had only a minor influence on separation. The method developed was successfully applied for primary screening of RTILs (>20), with in depth evaluation of substrates in 10 RTILs, for their evaluation as reaction media.
Lue
Bena-Marie
BM
National Food Institute, Technical University of Denmark, Søltofts Plads, Building 227, 2800 Kgs. Lyngby, Denmark.
Guo
Zheng
Z
Xu
Xuebing
X
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004951:Esterification; D005419:Flavonoids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry*; D015394:Molecular Structure
D005419:Flavonoids; D052578:Ionic Liquids