Characterizing the metabolic fingerprint and anti-inflammatory activity of Hypericum gentianoides.
In this paper we characterize the metabolic fingerprint and first reported anti-inflammatory activity of Hypericum gentianoides. H. gentianoides has a history of medical use by Native Americans, but it has been studied very little for biological activity. High-performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) analyses of a methanol extract show that H. gentianoides contains a family of over nine related compounds that have retention times, mass spectra, and a distinctive UV absorption spectra characteristic of certain acyl-phloroglucinols. These metabolites are abundant relative to other secondary products present in H. gentianoides, accounting for approximately 0.2 g per gram of dry plant tissue. H. gentianoides methanol extracts and a specific semipreparative HPLC fraction from these extracts containing the putative acyl-phloroglucinols reduce prostaglandin E 2 synthesis in mammalian macrophages.
Hillwig
Matthew L
ML
Center for Research on Botanical Dietary Supplements, Department of Genetics, Development, and Cell Biology, and Department of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011, USA. mhillwig@iastate.edu
Hammer
Kimberly D P
KD
Birt
Diane F
DF
Wurtele
Eve Syrkin
ES
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D020902:Hypericum / Q000737:chemistry* / Q000378:metabolism; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D000432:Methanol; D051379:Mice; D010696:Phloroglucinol / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization
D000893:Anti-Inflammatory Agents; D010936:Plant Extracts; D010696:Phloroglucinol; D015232:Dinoprostone; D000432:Methanol