Cells captured from spatium intermusculare by porous material exhibit the characteristics of stem cells.
Most of the current methods to capture stem cells are very complicated. Our new discovery of acquiring adult stem cells by implanting three dimension (3-D) porous material into the spatium intermusculare of mice hind limbs would bring hope to achieve autologous stem cells transplantation. We discovered that a great number of cells migrated into the 3-D porous material implanted in vivo. Furthermore, the migrating cells exhibited stem cell properties (CD34(+), Sca-1(+), GFAP(+), alphafetoprotein(+)) and were hematogeous (CD45(+)) and CD105(+). The ability of migrating cells to undergo differentiation into hematopoietic lineages was tested with methylcellulose medium. These findings demonstrate that the cells captured from spatium intermusculare by implanting 3-D porous material exhibit the characteristics of stem cells.
Li
Junling
J
Shandong Medicinal Biotechnology Center, Shandong Academy of Medical Sciences, Jinan 250062, People's Republic of China. lijunling00@sina.com
Wang
Shili
S
Han
Jinxiang
J
Yu
Shiguang
S
Zhang
Cui
C
Zhao
Yan
Y
D000818:Animals; D000071063:Endoglin; D005904:Glial Fibrillary Acidic Protein; D006614:Hindlimb / Q000166:cytology* / Q000378:metabolism; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins / Q000032:analysis; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000032:analysis; D016062:Porosity; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D000509:alpha-Fetoproteins / Q000032:analysis
D000071063:Endoglin; C502004:Eng protein, mouse; D005904:Glial Fibrillary Acidic Protein; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D000509:alpha-Fetoproteins; C528900:glial fibrillary astrocytic protein, mouse