Binding properties of positively charged deoxynucleic guanidine (DNG), AgTgAgTgAgT and DNG/DNA chimeras to DNA.
The melting properties of hexameric oligonucleotide AgTgAgTgAgT, in which the phosphodiester linkages of the DNA have been replaced by guanidium linkages, have been evaluated. Using the juvenile esterase gene as a target, the binding of a 20-mer DNG/DNA chimera that includes AgTgAgTgAgT is more than 10(5.7) stronger than the binding of 20-mer composed solely of DNA.
Department of Chemistry and Biochemistry, University of Califonia at Santa Barbara, CA 93106, USA.
Myunji
M
Park
Thomas C
TC
Bruice
D001665:Binding Sites; D001667:Binding, Competitive; D002265:Carboxylic Ester Hydrolases / Q000737:chemistry; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D006146:Guanidines / Q000138:chemical synthesis / Q000737:chemistry*; D008970:Molecular Weight; D009690:Nucleic Acid Conformation; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis / Q000737:chemistry*; D010755:Organophosphates / Q000737:chemistry; D013696:Temperature
D006146:Guanidines; D009841:Oligonucleotides; D010755:Organophosphates; C088820:deoxynucleic guanidine; D004247:DNA; D002265:Carboxylic Ester Hydrolases; C038003:juvenile hormone esterase