A homemade device for linear sucrose gradients.
We have developed a simple and inexpensive device to obtain linear sucrose gradients with commonly used laboratory materials--a syringe, a flask, a plastic tube, and a piece of Pongo (Play-Doh). Refractive index values measured on sucrose fractions collected using our system demonstrate both the linearity and reliability of the gradients obtained.
Bellavia
Daniele
D
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, 90128 Palermo, Italy.
Cellura
Doriana
D
Sisino
Giorgia
G
Barbieri
Rainer
R
D002499:Centrifugation, Density Gradient / Q000191:economics / Q000295:instrumentation*; D003365:Costs and Cost Analysis; D007753:Laboratories; D015203:Reproducibility of Results