Agromyces terreus sp. nov., isolated from soil.
A Gram-positive, non-motile, cream-coloured, curved or straight rod-shaped bacterial strain, DS-10(T), was isolated from soil of Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain DS-10(T) grew optimally at 25 degrees C and pH 7.0-8.0 in the presence of 0.5-1.0 % (w/v) NaCl. Strain DS-10(T) had peptidoglycan type B2gamma [d-Glu-d-Dab (diaminobutyric acid)] and galactose and ribose as whole-cell sugars. Strain DS-10(T) contained MK-11, MK-12 and MK-10 as predominant menaquinones. Major cellular fatty acids (>10 % of total) were anteiso-C(15 : 0), anteiso-C(17 : 0), iso-C(16 : 0) and iso-C(15 : 0). Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid. The DNA G+C content was 71.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-10(T) belonged to the genus Agromyces. Similarity values between the 16S rRNA gene sequence of strain DS-10(T) and those of the type strains of recognized Agromyces species ranged from 95.8 to 99.3 %. DNA-DNA relatedness data and differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain DS-10(T) could be differentiated from recognized Agromyces species. On the basis of the data presented, strain DS-10(T) is considered to represent a novel species of the genus Agromyces, for which the name Agromyces terreus sp. nov. is proposed. The type strain is DS-10(T) (=KCTC 19216(T) =JCM 14581(T)).
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea. jhyoon@kribb.re.kr
Jung-Hoon
JH
Yoon
Peter
P
Schumann
So-Jung
SJ
Kang
Sooyeon
S
Park
Tae-Kwang
TK
Oh
D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D005838:Genotype; D007723:Korea; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S