Design of a system to measure light scattering from individual cells excited by an acoustic wave.
A system to measure light scattering from individual cells excited by an acoustic wave was designed, and tests were performed on live Jurkat cells. Cells passing in a laminar stream within a water bath were excited by a focused ultrasound pulse, while the scattered light from a laser beam was monitored at various scattering angles. The cells were modeled as viscoelastic liquid drops, which return to equilibrium via shape oscillations after an acoustically-induced deformation. The Fast Fourier Transform of the scattered light signal was used to extract information about the highly-damped resonant frequencies of the cells, and the detected frequencies are consistent with theoretical predictions.
Georgescu
Ramona
R
Boston University, Dept. of Electrical & Computer Engineering, Boston, MA 02215, USA.
Khismatullin
Damir
D
Holt
R Glynn
RG
Castagner
Jean Luc
JL
A'amar
Ousama
O
Bigio
Irving J
IJ
D000162:Acoustics; D002469:Cell Separation / Q000295:instrumentation*; D054459:Elasticity Imaging Techniques / Q000295:instrumentation* / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005434:Flow Cytometry / Q000295:instrumentation*; D006801:Humans; D019169:Jurkat Cells; D008027:Light; D033401:Microscopy, Acoustic / Q000295:instrumentation*; D012542:Scattering, Radiation