[Relationship between the gene mutations of mannose binding protein and the progression of hepatitis B infection].
OBJECTIVE
To investigate the relationship between the gene mutations of mannose binding protein(MBP) and the progression of hepatitis B.


METHODS
The MBP gene mutations in 52 patients with chronic hepatitis B and 62 patients with severe hepatitis B and 64 HBsAg-negative healthy controls were investigated. The mutations in MBP gene were analyzed by polymerase chain reaction (PCR) and direct DNA sequencing.


RESULTS
A mutation of MBP gene codon 54 was found. The mutation frequency in the group of severe hepatitis B (35.5%, 22/62) was higher than those in the chronic hepatitis B group (15.4%, 8/52) and the HBsAg-negative healthy controls(14.1%, 9/64), respectively, and their difference was significant (chi-square was 7.79, P< 0.01; chi-square was 5.89,lzP<0.05). The difference between the chronic hepatitis B group and the HBsAg-negative healthy control group was not significant (P > 0.05).


CONCLUSIONS
There is only mutation in codon 54 of the MBP gene in patients with hepatitis B infection in the area analyzed. Codon 54 mutation of MBP gene is not related to the persistence of hepatitis B, but it was associated with the progression of hepatitis B infection.
Tong
Fu-yi
FY
Institute of Hepatology, Suzhou Fifth Peopleos Hospital, Suzhou, Jiangsu, 215007 People's Republic of China. tongfy@163.com
Gan
Jian-he
JH
Lu
Qin
Q
Cao
Wen-gui
WG
Shen
Xiu-juan
XJ
D000328:Adult; D000368:Aged; D003062:Codon / Q000235:genetics; D005260:Female; D006509:Hepatitis B / Q000235:genetics*; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000235:genetics*; D008875:Middle Aged; D009154:Mutation / Q000235:genetics*; D016133:Polymerase Chain Reaction; D055815:Young Adult
D003062:Codon; D037601:Mannose-Binding Lectin