[Determination of rubrofusarin gentiobioside in Cassia obtusifolia by HPLC].
OBJECTIVE
To establish a method for determining the content of rubrofusarin gentiobioside in Cassia obtusifolia.


METHODS
The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, acetonitrile-THF-1% acetic acid (18: 3:79) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees 2 and detection wavelength at 278 nm.


RESULTS
A good linearity was obtained from 0.1-0.5 microg with r = 0.999 9 for rubrofusarin gentiobioside. The average recovery was 101.1%, and RSD was 2.23% (n = 5).


CONCLUSIONS
The method was proved to be simple, rapid, sensitive, precise, reliable and repeatable. It can be applied to the quality control of Semen Cassia.
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Li-Ying
LY
Tang
Zhu-Ju
ZJ
Wang
Qiu-Ping
QP
Wu
Yan
Y
He
Lu-Qi
LQ
Huang
D002366:Cassia / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002867:Chromones / Q000032:analysis* / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005960:Glucosides / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results
D002867:Chromones; D004365:Drugs, Chinese Herbal; D005960:Glucosides; C119595:rubrofusarin gentiobioside