Isolation, characterization of heavy metal resistant strain of Pseudomonas aeruginosa isolated from polluted sites in Assiut city, Egypt.
Sixty six isolates of Pseudomonas spp. were isolated from wastewater of El-Malah canal located in Assiut, Egypt and were checked for their heavy metal tolerance. One isolate has tested for its multiple metal resistances and found to be plasmid mediated with molecular weight 27 Kb for nickel and lead. It was identified as Pseudomonas aeruginosa ASU 6a. Its minimal inhibitory concentration (MIC) for Cu(2+), Co(2+), Ni(2+), Zn(2+), Cr(3+), Cd(2+)and Pb(2+) were 6.3, 5.9, 6.8, 9.2, 5.8, 4.4, and 3.1 mM, respectively. Growth kinetics and the maximum adsorption capacities were determined under Ni(2+) and Pb(2+) stress. The latter heavy metals induced potassium efflux and were used as indicator for plasma membrane permeabilization.
University of Assiut, Faculty of Science, Botany Department, Assiut, Egypt.
S H A
SH
Hassan
R N N
RN
Abskharon
S M F Gad
SM
El-Rab
A A M
AA
Shoreit
D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000378:metabolism; D002463:Cell Membrane Permeability; D024901:Drug Resistance, Multiple, Bacterial; D004534:Egypt; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D007700:Kinetics; D007854:Lead / Q000378:metabolism / Q000494:pharmacology; D015227:Lipid Peroxidation; D019216:Metals, Heavy / Q000378:metabolism / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009532:Nickel / Q000378:metabolism / Q000494:pharmacology; D010957:Plasmids; D011188:Potassium / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification*; D014169:Transformation, Bacterial; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D019216:Metals, Heavy; D014874:Water Pollutants, Chemical; D007854:Lead; D009532:Nickel; D011188:Potassium