The stromal cell-derived factor-1alpha dependent migration of human cord blood CD34 haematopoietic stem and progenitor cells switches from protein kinase C (PKC)-alpha dependence to PKC-alpha independence upon prolonged culture in the presence of Flt3-ligand and interleukin-6.
Addition of the inflammatory cytokine interleukin (IL)-6 to the culture medium of human cord blood haematopoietic stem and progenitor cells (HSPCs) has been shown to lead to an altered stromal cell-derived factor-1alpha-dependent migratory phenotype. This study investigated whether this effect was attributed to a differential engagement of protein kinase C (PKC) isotypes. The migratory activity of both Flt3-ligand and Flt3-ligand/IL-6 cultured cord blood HSPCs was PKC-alpha dependent on day 1, but PKC-alpha independent after 5 d of cultivation. PKC-alpha expression was not down-regulated in cells cultured for 5 d indicating a switch of signalling molecules directing cell migration.
Institute of Immunology, Witten/Herdecke University, Witten, Germany.
Benjamin
B
Kasenda
Susannah H
SH
Kassmer
Bernd
B
Niggemann
Sven
S
Schiermeier
Wolfgang
W
Hatzmann
Kurt S
KS
Zänker
Thomas
T
Dittmar
D002465:Cell Movement / Q000502:physiology*; D054377:Chemokine CXCL12 / Q000494:pharmacology; D005312:Fetal Blood / Q000166:cytology*; D006412:Hematopoietic Stem Cells / Q000166:cytology*; D006801:Humans; D015850:Interleukin-6; D008565:Membrane Proteins; D051571:Protein Kinase C-alpha / Q000378:metabolism*; D013234:Stem Cells / Q000166:cytology*
D054377:Chemokine CXCL12; D015850:Interleukin-6; D008565:Membrane Proteins; C084881:flt3 ligand protein; D051571:Protein Kinase C-alpha