Detection of porcine circovirus type 2 in pigs imported from Indonesia.
We have detected the presence of porcine circovirus (PCV) type 2 in Indonesian pigs imported to Singapore for food consumption. A total of three viral isolates were identified, and to genetically characterise them further, their full genomes were sequenced. Each genome showed a typical organization of PCV type 2, with the three isolates sharing similar genome lengths of 1767 nucleotide (nt) at high nt identities of 99.8-100%, further indicating that the viral isolates were quite homogeneous. Sequence analysis further revealed that the ORF2 genes contain the nt sequence CCCCGC (from nt position 262 to 267) that was previously reported to be associated with PCV type 2, group 1C. The phylogenetic tree was constructed for the ORF2 genes, and the PCV type 2 isolates distributed into two distinctive groups. The Indonesian PCV type 2 clustered tightly with one China isolate, accession number AY035820, as a sub-cluster in group 1C. The sequence and phylogenetic analyses both confirmed that the three Indonesian PCV type 2 isolates belong to group 1C, and that the genetic changes for the three Indonesian isolates were very stable, possibly due to the low-scale evolution.
Detection and Diagnostics Laboratory, DSO National Laboratories, #13-00, 27 Medical Drive, Singapore.
Gayathri
G
Manokaran
Yueh-Nuo
YN
Lin
Moi-Lien
ML
Soh
Elizabeth Ai-Sim
EA
Lim
Chee-Wee
CW
Lim
Boon-Huan
BH
Tan
D000818:Animals; D018173:Circoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D007214:Indonesia / Q000453:epidemiology; D038622:Internationality; D010802:Phylogeny; D012846:Singapore / Q000453:epidemiology; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000821:virology*