Implanting hydroxyapatite-coated porous titanium with bone morphogenetic protein-2 and hyaluronic acid into distal femoral metaphysis of rabbits.
OBJECTIVE
To assess the osseointegration capability of hydroxyapatite-coated porous titanium with bone morphogenetic protein-2 (BMP-2) and hyaluronic acid to repair defects in the distal femur metaphysis in rabbits.


METHODS
Porous titanium implants were made by sintering titanium powder at high temperature, which were coated with hydroxyapatite by alkali and heat treatment and with BMP-2 combined with bone regeneration materials. And hyaluronic acid was further used as delivery system to prolong the effect of BMP-2. The implants were inserted into the metaphysis of the distal femur of rabbits. The animals were killed at 6, 12 and 24 weeks to accomplish histological and biomechanical analyses.


RESULTS
According to the result of histological analysis, the osseointegration in BMP-2 group was better than that of the HA-coated porous titanium group. In push-out test, all the samples had bigger shear stress as time passed by. There was statistical difference between the two groups in 6 and 12 weeks but not in 24 weeks.


CONCLUSIONS
Hydroxyapatite-coated porous titanium with BMP-2 and hyaluronic acid has a good effect in repairing defects of distal femur in rabbits, which is a fine biotechnology for future clinical application.
Department of Orthopedic Surgery, Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical College, Wenzhou 325000, China.
Lei
L
Peng
Wei-guo
WG
Bian
Fang-hui
FH
Liang
Hua-zi
HZ
Xu
D000818:Animals; D001696:Biomechanical Phenomena; D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible; D017886:Durapatite; D005269:Femur / Q000601:surgery; D006820:Hyaluronic Acid / Q000494:pharmacology*; D016348:Osseointegration / Q000502:physiology*; D016062:Porosity; D019736:Prostheses and Implants; D011817:Rabbits; D014025:Titanium; D016212:Transforming Growth Factor beta / Q000494:pharmacology*
D055396:Bone Morphogenetic Protein 2; D019485:Bone Morphogenetic Proteins; D020099:Coated Materials, Biocompatible; D016212:Transforming Growth Factor beta; D006820:Hyaluronic Acid; D017886:Durapatite; D014025:Titanium