Multiplex fast real-time PCR for quantitative detection and identification of cos- and pac-type Streptococcus thermophilus bacteriophages.
The fermentation of milk by Streptococcus thermophilus is a widespread industrial process that is susceptible to bacteriophage attack. In this work, a preventive fast real-time PCR method for the detection, quantification, and identification of types of S. thermophilus phages in 30 min is described.
del Rio
Beatriz
B
Instituto de Productos Lácteos de Asturias (CSIC), 33300 Villaviciosa, Asturias, Spain.
Martín
María Cruz
MC
Martínez
Noelia
N
Magadán
Alfonso H
AH
Alvarez
Miguel A
MA
D017931:DNA Primers; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D015183:Restriction Mapping; D012680:Sensitivity and Specificity; D017108:Streptococcus Phages / Q000145:classification / Q000235:genetics*; D048249:Streptococcus thermophilus / Q000821:virology*
D017931:DNA Primers