[Study on enzyme-assisted extraction of polysaccharides from Dioscorea opposita].
OBJECTIVE
To extract polysaccharides from Dioscorea opposita by alpha-amylase and ultrasound.


METHODS
The optimum condition of enzyme-assisted extraction has been obtained through orthogonal test according to the yield of polysaccharides.


RESULTS
A higher yield of polysaccharides was achieved at 55 degrees C, pH 5.5 , with a load of alpha-amylase 10 mg for 1.0 hour, the extraction rate was increased by in compare of sonolysis treatment alone.


CONCLUSIONS
To carry out an enzyme treatment before ultrasound--assisted extraction elevated the yield of polysaccharides.
Zhang
Yuan
Y
Biochemical Engineering College of Beijing Uion University, Beijing 100023, China.
Lin
Qiang
Q
Wei
Jing-Na
JN
Zhu
Hong-Ji
HJ
D027742:Dioscorea / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011134:Polysaccharides / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism; D000516:alpha-Amylases / Q000378:metabolism*
D011134:Polysaccharides; D000516:alpha-Amylases