Improving the sensitivity of immunoassays by tuning gold nanoparticles to the tipping point.
Conventional lateral flow immunoassays are based on labeled antibodies. In this paper we describe an alternative design based on gold nanoparticles labeled with haptens. The haptens are conjugated to gold nanoparticles by a method that allows the number per particle to be tuned to the point of maximum sensitivity. This leads to improvements compared with conventional lateral flow devices without relinquishing any of their advantages. In parallel assays for the environmental pollutant 2,4-dinitrophenol the alternative devices were 50% more sensitive.
Aveyard
Jenny
J
Department of Chemistry, Liverpool University, Liverpool L69 7ZD, UK.
Nolan
Paul
P
Wilson
Robert
R
D019297:2,4-Dinitrophenol / Q000032:analysis; D004785:Environmental Pollutants / Q000032:analysis; D006046:Gold; D006241:Haptens; D007118:Immunoassay / Q000379:methods / Q000592:standards*; D053768:Metal Nanoparticles
D004785:Environmental Pollutants; D006241:Haptens; D006046:Gold; D019297:2,4-Dinitrophenol