Automated recombinant protein expression screening in Escherichia coli.
To fit the requirements of structural genomics programs, new as well as classical methods have been adapted to automation. This chapter describes the automated procedure developed within the Structural Biology and Genomics Platform, Strasbourg for performing recombinant protein expression screening in Escherichia coli. The procedure consists of parallel competent cells transformation, cell plating, and liquid culture inoculation, implemented for up to 96 samples at a time.
Busso
Didier
D
Structural Biology and Genomics Platform, IGBMC, CNRS/INSERM/Université Louis Pasteur, Illkirch, France.
Stierlé
Matthieu
M
Thierry
Jean-Claude
JC
Moras
Dino
D
D001331:Automation; D002474:Cell-Free System; D003001:Cloning, Molecular / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D005822:Genetic Vectors; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D014169:Transformation, Bacterial
D011994:Recombinant Proteins