Effects of chloramphenicol on brain energy metabolism using 31P spectroscopy: influences on sleep-wake states in rat.
Effects of chloramphenicol (antibiotic inhibiting complex-1 of respiratory chain) and thioamphenicol (TAP, a structural analog of CAP inactive on complex-1) were examined on cerebral energy metabolites and sleep-wake cycle architecture in rat. In the first group, animals were chronically equipped with a cranial surface resonator and (31)P spectroscopic measurements were performed using a 2 T magnetic resonance spectrometer (operating frequency 34.46 MHz). CAP administration (400 mg/kg, tail vein, light period) induced deficits in phosphocreatine (-30%, p < 0.01) and ATP (-40%, p < 0.01), whereas TAP (400 mg/kg) had no effect. In the second group, animals were chronically implanted with polygraphic electrodes for EEG and electromyogram recordings. CAP administered intraperitoneally at light-onset reduced rapid-eye movement (REM) sleep (-60% in the first 6 h of light period, p < 0.01), increased waking state (+65% in the first 6 h of light period, p < 0.01), and slightly affected slow-wave sleep (SWS). During waking state, theta and sigma power bands of the EEG were, respectively, increased and decreased (p < 0.05). During SWS, delta power band was reinforced (p < 0.05), while theta, alpha, and sigma bands were decreased (p < 0.05). No changes occurred during REM sleep. TAP had no effect on sleep-wake states and spectral components of the EEG. Overall, these data indicate that REM sleep occurrence is linked to an aerobic production of ATP.
Chahboune
Halima
H
Anlyan Center for Medical Research, Department of Diagnostic Radiology, Yale University School of Medicine, New Haven, Connecticut, USA.
Mahdjoub
Rachid
R
Desgoutte
Pierre
P
Rousset
Colette
C
Briguet
André
A
Cespuglio
Raymond
R
D000255:Adenosine Triphosphate / Q000172:deficiency / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002701:Chloramphenicol / Q000494:pharmacology*; D042967:Electron Transport Complex I / Q000172:deficiency / Q000378:metabolism; D004734:Energy Metabolism / Q000187:drug effects / Q000502:physiology*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D010725:Phosphocreatine / Q000172:deficiency / Q000378:metabolism; D010761:Phosphorus Radioisotopes / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012890:Sleep / Q000187:drug effects / Q000502:physiology*; D012894:Sleep Stages / Q000187:drug effects / Q000502:physiology; D014851:Wakefulness / Q000187:drug effects / Q000502:physiology*
D010761:Phosphorus Radioisotopes; D010725:Phosphocreatine; D002701:Chloramphenicol; D000255:Adenosine Triphosphate; D042967:Electron Transport Complex I