Application of lipidomics and metabolomics to the study of adipose tissue.
Role of specific reactive lipids as well as amino acids in control of insulin signalling in adipose tissue is well recognized. Since it is practically impossible to measure the levels of all metabolites in the biological sample simultaneously with a single analytical platform, we utilize multiple platforms to study the lipids and metabolites of relevance to adipose tissue metabolism and insulin signalling. Two screening platforms cover a broad range of lipid molecular species (UPLC/MS based lipidomics platform) as well as organic acids and sterols (GCxGC-TOF platform). A targeted platform for amino acids (UPLC) is also applied.
Mattila
Ismo
I
VTT Technical Research Centre of Finland, Tietotie 2, FI-02044 VTT, Espoo, Finland.
Seppänen-Laakso
Tuulikki
T
Suortti
Tapani
T
Oresic
Matej
M
D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D007328:Insulin / Q000378:metabolism; D050356:Lipid Metabolism; D008055:Lipids / Q000032:analysis*; D046228:Microarray Analysis / Q000295:instrumentation / Q000379:methods; D015398:Signal Transduction / Q000502:physiology
D007328:Insulin; D008055:Lipids