Localised axial progenitor cell populations in the avian tail bud are not committed to a posterior Hox identity.
The outgrowth of the vertebrate tail is thought to involve the proliferation of regionalised stem/progenitor cell populations formed during gastrulation. To follow these populations over extended periods, we used cells from GFP-positive transgenic chick embryos as a source for donor tissue in grafting experiments. We determined that resident progenitor cell populations are localised in the chicken tail bud. One population, which is located in the chordoneural hinge (CNH), contributes descendants to the paraxial mesoderm, notochord and neural tube, and is serially transplantable between embryos. A second population of mesodermal progenitor cells is located in a separate dorsoposterior region of the tail bud, and a corresponding population is present in the mouse tail bud. Using heterotopic transplantations, we show that the fate of CNH cells depends on their environment within the tail bud. Furthermore, we show that the anteroposterior identity of tail bud progenitor cells can be reset by heterochronic transplantation to the node region of gastrula-stage chicken embryos.
The Roslin Institute and Royal Dick School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, UK.
Michael J
MJ
McGrew
Adrian
A
Sherman
Simon G
SG
Lillico
Fiona M
FM
Ellard
Pippa A
PA
Radcliffe
Hazel J
HJ
Gilhooley
Kyriacos A
KA
Mitrophanous
Noemí
N
Cambray
Valerie
V
Wilson
Helen
H
Sang
D000818:Animals; D030801:Animals, Genetically Modified; D002452:Cell Count; D002454:Cell Differentiation; D002642:Chick Embryo; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D013623:Tail / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*
D018398:Homeodomain Proteins