Lifeact: a versatile marker to visualize F-actin.
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
Riedl
Julia
J
Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany.
Crevenna
Alvaro H
AH
Kessenbrock
Kai
K
Yu
Jerry Haochen
JH
Neukirchen
Dorothee
D
Bista
Michal
M
Bradke
Frank
F
Jenne
Dieter
D
Holak
Tad A
TA
Werb
Zena
Z
Sixt
Michael
M
Wedlich-Soldner
Roland
R
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004396:Coloring Agents / Q000737:chemistry*; D003599:Cytoskeleton / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D000199:Actins; D004396:Coloring Agents; D010455:Peptides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins