Light- and IAA-regulated ACC synthase gene (PnACS) from Pharbitis nil and its possible role in IAA-mediated flower inhibition.
The light- and indole-3-acetic acid (IAA)-regulated 1-aminocyclopropane-1-carboxylic acid (ACC) synthase gene (PnACS) from Pharbitis nil was isolated. Here, it was shown that the gene was expressed in cotyledons, petioles, hypocotyls, root and shoot apexes both in light- and dark-grown seedlings. The highest expression level of PnACS was found in the roots. IAA applied to the cotyledons of P. nil seedlings caused a clear increase of PnACS messenger accumulation in all the organs examined. In this case, the most IAA-responsive were the hypocotyls. Our studies revealed that the PnACS transcript level in the cotyledons exhibited diurnal oscillations under both long-day (LD) and short-day (SD) conditions. IAA applied at the beginning of inductive darkness caused a dramatic increase in the expression of PnACS, suggesting that the inhibitory effect of IAA on P. nil flowering may result from its stimulatory effect on ethylene production.
Department of Physiology and Molecular Biology of Plants, Institute of General and Molecular Biology, Nicolaus Copernicus University, 9 Gagarina Street, 87-100 Torun, Poland.
Kamil
K
Frankowski
Jacek
J
Kesy
Waldemar
W
Wojciechowski
Jan
J
Kopcewicz
D000595:Amino Acid Sequence; D001483:Base Sequence; D018548:Cotyledon / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects; D018076:DNA, Complementary / Q000302:isolation & purification; D035264:Flowers / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects / Q000528:radiation effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects* / Q000528:radiation effects; D007210:Indoleacetic Acids / Q000494:pharmacology*; D049915:Ipomoea nil / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics* / Q000528:radiation effects; D008027:Light; D008190:Lyases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D009928:Organ Specificity / Q000187:drug effects / Q000528:radiation effects; D017440:Photoperiod; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D036226:Seedlings / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000528:radiation effects
D018076:DNA, Complementary; D007210:Indoleacetic Acids; D012333:RNA, Messenger; C030737:indoleacetic acid; D008190:Lyases; C021773:1-aminocyclopropanecarboxylate synthase