Differential display of grouper iridovirus-infected grouper cells by immunostaining.
Grouper iridovirus (GIV) is one of the most devastating infectious pathogens of aquaculture fish. When infecting a susceptible cell line, such as GK-2, GIV causes antigenic changes in host cellular proteins. To understand the host gene expression characteristics after viral infection, we developed an immunostaining method to screen differentially expressed genes of fish cells in response to GIV infection using phage display complementary DNA libraries. In total, 66 genes were identified from grouper kidney and brain cell lines. These genes are related to replication, transcription, translation, immunity, apoptosis, structure proteins, metabolism, energy, protein modification, and homeostasis. Four dynamic antigenic patterns were observed among these immunocloned genes upon GIV infection. Microarray analysis further confirmed the transcriptional patterns of 80% of the identified genes. This immunostaining screening method provides insights into a host's cellular protein response to viral infection on a translational basis.
Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan, ROC.
Chiao-Hwa
CH
Yeh
Yao-Sheng
YS
Chen
Ming-Shan
MS
Wu
Chien-Wen
CW
Chen
Chung-Hsiang
CH
Yuan
Kuo-Wen
KW
Pan
Ya-Nan
YN
Chang
Nin-Nin
NN
Chuang
Chi-Yao
CY
Chang
D000818:Animals; D000941:Antigens / Q000032:analysis; D001492:Bass / Q000235:genetics* / Q000821:virology*; D015153:Blotting, Western; D002460:Cell Line; D003588:Cytopathogenic Effect, Viral / Q000235:genetics; D005393:Fish Diseases / Q000235:genetics* / Q000821:virology*; D020869:Gene Expression Profiling / Q000379:methods*; D017994:Iridovirus
D000941:Antigens