The potential role of combined anti-oxidants against cadmium toxicity on liver of rats.
Cadmium (Cd), a widely distributed toxic trace metal, has been shown to accumulate in liver after long- and short-term exposure. Cd (2 mg/kg/day CdCl2) was intraperitoneally given to rats for eight days. Vitamin C (250 mg/kg/day) + vitamin E (250 mg/kg/day) + sodium selenate (0.25 mg/kg/day) were given to rats by oral means. The animals were treated by anti-oxidants one hour prior to treatment with Cd every day. The degenerative changes were observed in the groups given only Cd and anti-oxidants + Cd. Metallothionein (MT) immunoreactivity increased in cytoplasm of hepatocytes of the rats given Cd when compared with controls. In a number of cells with Cd and anti-oxidants treatment, immunoreactivity increase was more than in the group given Cd only and nuclear MT expression was also detected. Cell proliferation was assessed with proliferating cell nuclear antigen (PCNA) immunohistochemistry. PCNA expressions increased in all groups more than in the controls. Anti-oxidants treatment increased cell proliferation. In the animals administered with Cd, an increase in serum aspartate (AST) and alanine (ALT) aminotransferases, liver glutathione (GSH) and lipid peroxidation (LPO) levels were observed. On the other hand, in the rats treated with anti-oxidants and Cd, serum AST and ALT, liver glutathione and LPO levels decreased. As a result, these results suggest that combined anti-oxidants treatment might be useful in protection of liver against Cd toxicity.
Koyuturk
Meral
M
Department of Histology and Embryology, Faculty of Medicine, Istanbul Science University, 34394-Esentepe, Istanbul, Turkey. mkoyuturk1@yahoo.com
Yanardag
Refiye
R
Bolkent
Sehnaz
S
Tunali
Sevim
S
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001205:Ascorbic Acid / Q000494:pharmacology; D002104:Cadmium / Q000008:administration & dosage; D002105:Cadmium Poisoning / Q000188:drug therapy / Q000517:prevention & control*; D056486:Chemical and Drug Induced Liver Injury; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008107:Liver Diseases / Q000188:drug therapy / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D008668:Metallothionein / Q000096:biosynthesis; D018809:Proliferating Cell Nuclear Antigen / Q000096:biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D064586:Selenic Acid; D018036:Selenium Compounds / Q000494:pharmacology; D014810:Vitamin E / Q000494:pharmacology
D000975:Antioxidants; D018809:Proliferating Cell Nuclear Antigen; D018036:Selenium Compounds; D002104:Cadmium; D014810:Vitamin E; D008668:Metallothionein; D064586:Selenic Acid; D001205:Ascorbic Acid