Lab-on-a-chip technologies for proteomic analysis from isolated cells.
Lab-on-a-chip systems offer a versatile environment in which low numbers of cells and molecules can be manipulated, captured, detected and analysed. We describe here a microfluidic device that allows the isolation, electroporation and lysis of single cells. A431 human epithelial carcinoma cells, expressing a green fluorescent protein-labelled actin, were trapped by dielectrophoresis within an integrated lab-on-a-chip device containing saw-tooth microelectrodes. Using these same trapping electrodes, on-chip electroporation was performed, resulting in cell lysis. Protein release was monitored by confocal fluorescence microscopy.
Department of Electronics and Electrical Engineering, University of Glasgow, Glasgow G12 8QQ, UK. hs@elec.gla.ac.uk
H
H
Sedgwick
F
F
Caron
P B
PB
Monaghan
W
W
Kolch
J M
JM
Cooper
D000199:Actins / Q000032:analysis; D045744:Cell Line, Tumor; D002469:Cell Separation; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods*; D018274:Electroporation; D049452:Green Fluorescent Proteins / Q000032:analysis; D006801:Humans; D008856:Microscopy, Fluorescence; D040901:Proteomics / Q000379:methods*; D011993:Recombinant Fusion Proteins / Q000032:analysis
D000199:Actins; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins