Identification of a root-specific glycosyltransferase from Arabidopsis and characterization of its promoter.
A set of Ds-element enhancer trap lines of Arabidopsis thaliana was generated and screened for expression patterns leading to the identification of a line that showed root-specific expression of the bacterial uidA reporter gene encoding beta-glucuronidase (GUS). The insertion of the Ds element was found to be immediately downstream to a glycosyltransferase gene At1g73160. Analysis of At1g73160 expression showed that it is highly root-specific. Isolation and characterization of the upstream region of the At1g73160 gene led to the definition of a 218 bp fragment that is sufficient to confer root-specific expression. Sequence analysis revealed that several regulatory elements were implicated in expression in root tissue. The promoter identified and characterized in this study has the potential to be applied in crop biotechnology for directing the root-specific expression of transgenes.
Vijaybhaskar
Virupapuram
V
Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.
Subbiah
Veeraputhiran
V
Kaur
Jagreet
J
Vijayakumari
Pagadala
P
Siddiqi
Imran
I
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D018744:DNA, Plant; D018506:Gene Expression Regulation, Plant; D016695:Glycosyltransferases / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D018517:Plant Roots / Q000201:enzymology* / Q000235:genetics; D011401:Promoter Regions, Genetic
D029681:Arabidopsis Proteins; D018744:DNA, Plant; C531448:At1g73160 protein, Arabidopsis; D016695:Glycosyltransferases