Determination of 2,4,6-trichloroanisole and 2,4,6-tribromoanisole in wine using microextraction in packed syringe and gas chromatography-mass spectrometry.
A selective and fast method for the quantitative determination of 2,4,6-trichloroanisole (TCA) and 2,4,6-tribromoanisole (TBA) in wine was developed. Microextraction in packed syringe (MEPS) was optimized for the extraction and preconcentration of the analytes using extremely small volume samples (0.1-1 mL). For GC-EI-MS, the limit of detection (LOD) for red and white wine was in the range 0.17-0.49 microg L(-1) for TCA and TBA. In addition to GC-EI-MS both GC-NCI-MS and GC-HRMS were used to further improve both selectivity and sensitivity. The lowest LODs were achieved using GC-HRMS in the EI mode. In red and white wine samples the LODs were between 0.22-0.75 ng L(-1) for TCA and TBA. The reproducibility and linearity for the GC-HRMS method was good, with RSD-values of 4-10% for spiked red wine samples at 1 ng L(-1) and linearity with R (2) > 0.962 over a concentration range of 1 to 100 ng L(-1).
Department of Natural Sciences, Man-Technology-Environment Research Centre, Orebro University, 70182 Orebro, Sweden. sofie.jonsson@nat.oru.se
S
S
Jönsson
J
J
Hagberg
B
B
van Bavel
D000840:Anisoles / Q000032:analysis*; D008401:Gas Chromatography-Mass Spectrometry; D052617:Solid Phase Microextraction / Q000379:methods*; D013594:Syringes; D014920:Wine / Q000032:analysis*
D000840:Anisoles; C110228:2,4,6-trichloroanisole; C484217:2,4,6-tribromoanisole