Studies on 5alpha-androst-16-en-3-one binding to porcine serum, plasma and testicular cytosolic fraction and to human serum.
The present study evaluated whether a specific androstenone-binding protein is present in porcine and human serum, and in the cytosolic fraction of porcine testis. The binding of [(3)H]-androstenone to serum and testicular cytosol was measured in the absence (total binding) and presence (non-specific binding) of unlabelled androstenone. The optimization of the assay is described. As a part of the assay validation, the binding of [(3)H]-dihydrotestosterone ([(3)H]-DHT) to porcine and human serum was also examined. As expected, specific binding of [(3)H]-DHT was detected in human serum, but not in porcine serum. No specific androstenone-binding protein was detected, either in porcine or human serum, or in the cytosolic fraction of porcine testis. The amount of non-specific binding of [(3)H]-androstenone was slightly lower in porcine serum compared to human serum. Between-animal variations in [(3)H]-androstenone binding were studied in plasma samples from 15 animals with androstenone concentrations ranging from 1.1 to 23.1 ng/mL. Mean values+/-standard deviations of binding in these samples were 15.2+/-0.9% for total binding and 15.9+/-0.8% for non-specific bindings. Low between-animal variations indicate that androstenone binding does not affect androstenone accumulation in fat.
Department of Food Science, Swedish University of Agricultural Sciences, P.O. Box 7051, SE-750 07 Uppsala, Sweden. Galia.Zamaratskaia@lmv.slu.se
Galia
G
Zamaratskaia
Ellen
E
Dahl
Andrzej
A
Madej
E James
EJ
Squires
Oystein
O
Andresen
D000736:Androstenes / Q000097:blood / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay; D002458:Cell Fractionation; D003600:Cytosol / Q000378:metabolism*; D013196:Dihydrotestosterone / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D010949:Plasma / Q000378:metabolism*; D044967:Serum / Q000378:metabolism*; D034421:Sus scrofa; D013737:Testis / Q000378:metabolism*
D000736:Androstenes; D013196:Dihydrotestosterone; C000214:androst-16-en-3-one