[Effects of Siwu tang on serum protein of blood deficiency using proteomic technique].
OBJECTIVE
To research the effects of Siwu tang on serum protein of blood deficiency using proteomic technique and further explore its potential molecular mechanism to cure blood deficiency.


METHODS
The sera of normal, blood deficiency and cured group were collected. Proteomic protocol involving the high resolution two-dimensional polyacryamide gel electrophoresis, the computer-assisted image analysis, and the mass spectrometry was used to detect regulated protein by Siwu tang.


RESULTS
Compared with normal group, there were 15 proteins changed, in which 11 increased and 4 decreased expressed proteins in sera could be recovered by Siwu tang. The up-regulated proteins involved haptoglobin, clusterin, complement component C4B and GTP binding protein 2, while the down-regulated proteins involved transthyretin and heamoglobin beta.


CONCLUSIONS
Siwu tang could regulate serum protein, which include immunology, apoptosis, DNA injury repair, and blood ingredients. This might be the mechanism of Siwu tang to cure blood deficiency.
Yang
Ming-Hui
MH
Department of Traditional Chinese Medicine, General Hospital of PLA, Beijing 100853, China.
Ma
Zeng-Chun
ZC
Dou
Yong-Qi
YQ
Hu
Jian
J
Wang
Yu-Guang
YG
Tan
Hong-Ling
HL
Xiao
Cheng-Rong
CR
Gao
Yue
Y
D001798:Blood Proteins / Q000187:drug effects*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006402:Hematologic Diseases / Q000097:blood / Q000188:drug therapy; D006801:Humans; D040901:Proteomics / Q000379:methods*
D001798:Blood Proteins; D004365:Drugs, Chinese Herbal