Evaluation of the ADVIA 120 for analysis of canine cerebrospinal fluid.
BACKGROUND
Conventional techniques for canine cerebrospinal fluid (CSF) analysis require large sample volumes and are labor intensive and subject to operator variability.


OBJECTIVE
The purpose of this study was to evaluate the ADVIA120 CSF assay for analysis of canine CSF samples.


METHODS
CSF samples collected from 36 healthy control dogs and 17 dogs with neurologic disease were processed in parallel using the automated assay and established manual methods using a hemocytometer and cytocentrifugation. Results for WBC (total nucleated cell) count, RBC count, and differential nucleated cell percentages were compared using Spearman rank correlation coefficients and Bland-Altman bias plots.


RESULTS
Correlation coefficients for WBC and RBC counts were 0.57 and 0.83 for controls, and 0.92 and 0.94 for ill dogs, respectively. Coefficients for the percentages of neutrophils, lymphocytes, and monocytes were 0.53, 0.26, and 0.12 for controls and 0.77, 0.92, and 0.70 for dogs with neurologic disease. When data were combined (n=53), correlation coefficients were 0.86 and 0.91 for WBC and RBC counts, and 0.63, 0.43, and 0.30 for neutrophil, lymphocyte, and monocyte percentages. A 9.5% positive bias and 7.0% negative bias were obtained for the ADVIA 120 CSF assay for lymphocytes and macrophages in dogs with neurologic disease with Bland-Altman analysis. A 12.2% positive bias was found for lymphocyte percentage in dogs with neurologic disease.


CONCLUSIONS
Manual and automated CSF assays had moderate to excellent correlation for WBC and RBC concentrations, but results were more variable for differential cell percentages. The ADVIA assay may be more useful for assessment of canine CSF with adjustment of cell differentiation algorithms.
Department of Pathobiology, Ontario Veterinary College, University of Guelph, ON, Canada. kruotsal@lsd.uoguelph.ca
Kristiina
K
Ruotsalo
Roberto
R
Poma
Ronaldo C
RC
da Costa
Dorothee
D
Bienzle
D000818:Animals; D001322:Autoanalysis / Q000295:instrumentation / Q000662:veterinary; D016022:Case-Control Studies; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid / Q000662:veterinary*; D002555:Cerebrospinal Fluid / Q000166:cytology*; D004283:Dog Diseases / Q000134:cerebrospinal fluid*; D004285:Dogs; D004906:Erythrocyte Count / Q000662:veterinary; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation; D007958:Leukocyte Count / Q000662:veterinary; D008297:Male