Genotypic identification of Cryptosporidium spp. isolated from HIV-infected patients and immunocompetent children of São Paulo, Brazil.
Cryptosporidium isolates identified in fourteen stool samples, collected from five HIV-infected patients and nine immunocompetent children, living in the State of São Paulo, Brazil, were submitted to a molecular analysis using a nested PCR followed of restriction fragment length polymorphism (RFLP), for genetic characterization. The analysis was based on digestion with RsaI restriction enzyme of a DNA fragment amplified from the Cryptosporidium oocyst wall protein (COWP) gene. Based on this analysis, four samples were identified as Cryptosporidium parvum, eight as Cryptosporidium hominis and two presented a profile that corresponded to Cryptosporidium meleagridis when compared to the standards used in the analysis. The use of molecular methods can be helpful to identify source of infections and risk factors related to Cryptosporidium infection in our communities.
Araújo
Ana Julia Urias dos Santos
AJ
Instituto Básico de Biociências, Universidade de Taubaté, Avenida Tiradentes 500, Taubaté, SP, Brasil.
Kanamura
Herminia Yohko
HY
Almeida
Marcos Eduardo de
ME
Gomes
Aparecida Helena de Souza
AH
Pinto
Thais Helena Lemos
TH
Da Silva
Alexandre Januário
AJ
D017088:AIDS-Related Opportunistic Infections / Q000469:parasitology*; D000328:Adult; D000818:Animals; D001938:Brazil; D002648:Child; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics; D005243:Feces / Q000469:parasitology; D005838:Genotype; D006801:Humans; D007121:Immunocompetence; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D015800:Protozoan Proteins / Q000235:genetics*
D016054:DNA, Protozoan; D015800:Protozoan Proteins; C106305:oocyst wall protein, Cryptosporidium