Analysis of iridoids from Harpagophytum and eleutherosides from Eleutherococcus senticosus in horse urine.
LC/ESI-MS n methods have been previously set up to detect the administration of (i) Harpagophytum and (ii) preparations containing a plant capable of anti-stress properties: Eleutherococcus senticosus. Harpagoside has been found to be the main indicator of Harpagophytum administration in the horse. These methods have been applied to a large number of horse urine samples of various origins. Regarding the detection of Harpagophytum administration, harpagoside, harpagide and 8-para-coumaroyl harpagide were detected together in only one sample out of 317. Eleutheroside E was found to be the main indicator of Eleutherococcus senticosus administration. It was detected in post-administration samples collected from two horses having received a feed supplement containing Eleutherococcus senticosus for several days. Out of the 382 samples tested, eleutheroside E was found in an unexpected large number of urine samples (39%) of various origins and its presence cannot be only due to the sole use of herbal dietary supplements.
Colas
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Laboratoire des Courses Hippiques, 15 rue de Paradis, 91370 Verrières le Buisson, France.
Popot
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MA
Garcia
P
P
Bonnaire
Y
Y
Bouchonnet
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S
D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods; D028001:Eleutherococcus / Q000737:chemistry*; D006736:Horses; D039823:Iridoids / Q000652:urine*; D013058:Mass Spectrometry / Q000379:methods; D029601:Pedaliaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000652:urine*
D039823:Iridoids; D010936:Plant Extracts; C029073:eleutherosides