Supercritical extraction of carotenoids from Rosa canina L. hips and their formulation with beta-cyclodextrin.
The purpose of this research was to preliminary assess the suitability of a new method for the preparation of a solid formulation in form of powder composed by beta-cyclodextrin and the supercritical extract of Rosa canina hips. The method implies the extraction of carotenoids, in particular beta-carotene, from freeze dried fruits of R. canina with supercritical CO2 at 70 degrees C and 300 bar, in the presence of varying quantity of ethanol as entrainer. The obtained supercritical solution is then expanded at ambient conditions into an aqueous solution of beta-cyclodextrin to favour the interaction between beta-cyclodextrin and the lipophilic components of the extract. beta-carotene solubility (mole fraction) in supercritical CO2 or in supercritical CO2/ethanol mixtures were in the order of 1 10(-7). The beta-carotene extracted from R. canina fruits (nearly 10 microg/g of dry matrix), interacts almost quantitatively with beta-cyclodextrin affording a solid phase, which presents a low apparent solubility in water. Finally the interaction with beta-cyclodextrin results in a higher concentration of the beta-carotene trans- form relative to the cis- form in the extracted product when collected in an aqueous solution of beta-cyclodextrin with respect to the extract in n-hexane.
Department of Pharmacy, University of Parma, Viale G.P. Usberti 27/A, 43100 Parma, Italy.
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Tozzi
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Mulinacci
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Storlikken
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Pasquali
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Vincieri
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Bettini
D002245:Carbon Dioxide / Q000737:chemistry; D002338:Carotenoids / Q000737:chemistry* / Q000302:isolation & purification; D002626:Chemistry, Pharmaceutical; D025924:Chromatography, Supercritical Fluid; D004337:Drug Carriers; D000431:Ethanol / Q000737:chemistry; D005638:Fruit; D006586:Hexanes / Q000737:chemistry; D008956:Models, Chemical; D010865:Pilot Projects; D011208:Powders; D027826:Rosa / Q000737:chemistry; D012995:Solubility; D013678:Technology, Pharmaceutical / Q000379:methods*; D047392:beta-Cyclodextrins / Q000737:chemistry*
D004337:Drug Carriers; D006586:Hexanes; D011208:Powders; D047392:beta-Cyclodextrins; D002245:Carbon Dioxide; D002338:Carotenoids; D000431:Ethanol