Long term evaluation of functional and morphological bladder alterations on alloxan-induced diabetes and aging: experimental study in rats.
OBJECTIVE
to evaluate structural and functional effects of Alloxan- induced diabetes and aging on bladder of rats.


METHODS
evaluations were performed in three groups: A--8 weeks of age, B--44 weeks of age, C--44 weeks of age with alloxan-induced diabetes. Muscle layer thickness, extracellular matrix fibrosis and collagen were quantified on digital images of bladder samples. Cystometric evaluations before surgical vesical denervation (SVD), included maximum cystometric capacity (MCC), maximum bladder pressure (MBP), bladder contraction frequency (VCF), duration of bladder contraction (DC), threshold pressure (TP) and bladder compliance (BC). After SVD, maximum cystometric capacity (MCC), BC and maximum urethral closing pressure (MUCP) were also measured.


RESULTS
Reduced extracellular matrix fibrosis concentration and contraction strength were found in the bladders of group C. Before SVD, bladder compliance was not different between groups. Alterations were observed in MCC after SVD.


CONCLUSIONS
We did not notice smooth muscle hypertrophy in Alloxan-induced diabetic rats after 44 weeks. There was alteration in the total and relative amount of fibrosis and collagen. The cystometric studies support the idea that this morphological alterations are important to determine the different bladder functional patterns found in the aging and the Alloxan-induced diabetic animals.
Division of Urology, Department of Surgery and Anatomy, Ribeirão Preto Faculty of Medicine, University of São Paulo, SP, Brazil. aantunesrj@yahoo.com.br
Antonio Antunes
AA
Rodrigues
Haylton Jorge
HJ
Suaid
Silvio
S
Tucci
Valéria Paula Sassoli
VP
Fazan
Milton César
MC
Foss
Adauto José
AJ
Cologna
Antonio Carlos Pereira
AC
Martins
D000375:Aging / Q000473:pathology*; D000496:Alloxan; D000818:Animals; D003094:Collagen / Q000032:analysis; D053715:Cystotomy; D003714:Denervation / Q000009:adverse effects; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000473:pathology* / Q000503:physiopathology; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000473:pathology; D005355:Fibrosis / Q000473:pathology; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D053580:Muscle Strength / Q000502:physiology; D009130:Muscle, Smooth / Q000503:physiopathology; D009133:Muscular Atrophy / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D001743:Urinary Bladder / Q000294:innervation / Q000473:pathology* / Q000503:physiopathology
D000496:Alloxan; D003094:Collagen