[Synthesis of beta-amyloid fragment 5RHDSGY10 and its isomers].
Peptide RHDSGY that represents the fragment of human beta-amyloid Zn-binding site and its isomers RH(D-Asp)SGY and RH(beta-Asp)SGY have been prepared by solid-phase synthesis and analysed by HPLC and various mass-spectrometric methods. The problem of low yield of peptide RHDSGY and its isomers attributed to 9-fluorenylmethoxycarbonyl (Fmoc)-amino acids and/or formation of side-products as RH(Asp-imide)SGY and RHDSGY (instead of RH(beta-Asp)SGY) was solved via selection of reagents for the removal of Fmoc groups from the growing peptide chain.
Aleshina
E Iu
EIu
Pyndyk
N V
NV
Moĭsa
A A
AA
Sanzhakov
M A
MA
Kharybin
O N
ON
Nikolaev
E N
EN
Kolesanova
E F
EF
D016229:Amyloid beta-Peptides / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009842:Oligopeptides / Q000138:chemical synthesis* / Q000737:chemistry; D010446:Peptide Fragments / Q000138:chemical synthesis* / Q000737:chemistry; D011485:Protein Binding; D015032:Zinc
D016229:Amyloid beta-Peptides; D009842:Oligopeptides; D010446:Peptide Fragments; D015032:Zinc