Characterization of clonal relatedness among the natural population of Staphylococcus aureus strains by using spa sequence typing and the BURP (based upon repeat patterns) algorithm.
We evaluated the BURP (based upon repeat patterns) algorithm, which relies on sequencing of the Staphylococcus aureus protein A gene (spa), for its ability to infer clonal relatedness within a population of 110 wild-type strains. BURP clustering of the resulting 66 spa types was highly concordant with multilocus sequence typing (96.5% concordance) and pulsed-field gel electrophoresis (94.9%).
Institute for Hygiene, University Hospital Münster, Robert-Koch-Str. 41, 48149 Münster, Germany. mellmann@uni-muenster.de
Alexander
A
Mellmann
Thomas
T
Weniger
Christoph
C
Berssenbrügge
Ursula
U
Keckevoet
Alexander W
AW
Friedrich
Dag
D
Harmsen
Hajo
H
Grundmann
D015373:Bacterial Typing Techniques / Q000379:methods*; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D016521:Electrophoresis, Gel, Pulsed-Field; D005838:Genotype; D006801:Humans; D017422:Sequence Analysis, DNA / Q000379:methods; D013205:Staphylococcal Protein A / Q000235:genetics*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics*
D004269:DNA, Bacterial; D013205:Staphylococcal Protein A