An efficient use of the WATERGATE W5 sequence for observing a ligand binding with a protein receptor.
An efficient pulse sequence for observing a ligand binding with a receptor has been developed by incorporating the WATERGATE W5 sequence. In the conventional water ligand observed via gradient spectroscopy (WaterLOGSY) techniques, the water resonance is selectively excited using, e.g. the double-pulsed field gradient spin-echo (DPFGSE) sequence at the initial portion of pulse sequence. In the current version, the modified WATERGATE W5 sequence is incorporated at the initial portion of the pulse sequence, and the resonance at the water frequency can be selectively reserved by the modified WATERGATE W5 sequence. The efficiency of ligand-observed NMR screening techniques has been demonstrated using the human serum albumin (HSA)-tryptophan complex.
Furihata
Kazuo
K
Division of Agriculture and Agricultural Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Shimotakahara
Sakurako
S
Tashiro
Mitsuru
M
D001665:Binding Sites; D006801:Humans; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards; D011522:Protons; D012015:Reference Standards; D012709:Serum Albumin / Q000737:chemistry*; D014364:Tryptophan / Q000737:chemistry*; D014867:Water / Q000737:chemistry*
D008024:Ligands; D011522:Protons; D012709:Serum Albumin; D014867:Water; D014364:Tryptophan