Association of E1AF mRNA expression with tumor progression and matrilysin in human rectal cancer.
OBJECTIVE
To examine E1AF mRNA expression and to determine whether it is correlated with tumor progression and matrilysin in human rectal cancer.


METHODS
Real-time RT-PCR was used to determine E1AF and matrilysin expression in 100 matched rectal cancers and normal tissues.


RESULTS
Among the 100 rectal cancers, 69 cases of E1AF mRNA overexpression were observed. E1AF mRNA overexpression correlated well with matrilysin. In carcinomas, E1AF mRNA overexpression correlated significantly with depth of invasion, lymph node metastasis, venous involvement and advanced pTNM stage.


CONCLUSIONS
E1AF was correlated significantly with tumor progression of human rectal cancer and may be an important factor in rectal cancer progression.
Institute of Digestive Surgery and Organ Microcirculation, Department of Gastrointestinal Surgery, Sichuan University, Chengdu, China.
Hai-Yi
HY
Liu
Bin
B
Zhou
Ling
L
Wang
Yuan
Y
Li
Zong-Guang
ZG
Zhou
Xiao-Feng
XF
Sun
Bing
B
Xu
Yu-Jian
YJ
Zeng
Jun-Min
JM
Song
Hong-Zhi
HZ
Luo
Lie
L
Yang
D017500:Adenovirus E1A Proteins / Q000235:genetics*; D000368:Aged; D018450:Disease Progression; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D020783:Matrix Metalloproteinase 7 / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger / Q000235:genetics*; D012334:RNA, Neoplasm / Q000235:genetics; D012004:Rectal Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D012007:Rectum / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction
D017500:Adenovirus E1A Proteins; C079853:ETV4 protein, human; D011518:Proto-Oncogene Proteins; D050783:Proto-Oncogene Proteins c-ets; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7