Selective cyclooxygenase inhibitors prevent the growth of Chlamydia pneumoniae in HL cells.
The effects of the selective cyclooxygenase (COX) inhibitors SC-560 and PTPBS were studied in Chlamydia pneumoniae-infected HL cell cultures. Chlamydia pneumoniae growth and viability were assessed by quantifying inclusions and re-passages. COX-1 and COX-2 mRNA expression in HL cells during chlamydial infection was quantified with real-time polymerase chain reaction. SC-560 (10 microg/mL) and PTPBS (18 microg/mL) completely inhibited the growth of C. pneumoniae and the effect was dose-dependent between 4-9 microg/mL and 2-16 microg/mL, respectively. Inclusion size was reduced from 11.5+/-1.3 microm to 1.9+/-0.7 microm in the presence of the drugs. Removing the drugs returned the size to normal and increased the number of inclusions. Selective COX inhibitors appear to have a chlamydiostatic but not chlamydiacidic effect; they inhibit the growth of C. pneumoniae in vitro but do not prevent infection or eradicate C. pneumoniae from host cells.
Department of Medical Microbiology, University of Oulu, Aapistie 5A, Oulu, Finland. ying.yan@oulu.fi
Ying
Y
Yan
Sylvi
S
Silvennoinen-Kassinen
Liisa
L
Törmäkangas
Maija
M
Leinonen
Pekka
P
Saikku
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000187:drug effects* / Q000254:growth & development; D051545:Cyclooxygenase 1 / Q000096:biosynthesis; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D006801:Humans; D002479:Inclusion Bodies / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology*; D050296:Microbial Viability; D011720:Pyrazoles
D000900:Anti-Bacterial Agents; D016861:Cyclooxygenase Inhibitors; D011720:Pyrazoles; C115461:SC 560; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human