Nesterenkonia halophila sp. nov., a moderately halophilic, alkalitolerant actinobacterium isolated from a saline soil.
A Gram-positive, non-motile, moderately halophilic, alkalitolerant actinobacterium, designated strain YIM 70179(T), was isolated from a saline soil sample collected from Xinjiang Province, north-west China, and was subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan type of strain YIM 70179(T) was A4alpha, l-Lys-Gly-l-Glu. Cells of the isolate contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unknown glycolipid, MK-8 as major menaquinone and anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) as major fatty acids. The DNA G+C content was 68.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 70179(T) fell within the radiation of species of the genus Nesterenkonia. Levels of 16S rRNA gene sequence similarity between strain YIM 70179(T) and the type strains of recognized Nesterenkonia species were below 97 %, except to Nesterenkonia halobia DSM 20541(T) (99.6 %), but these two strains exhibited a low level of DNA-DNA relatedness (18.4 %). Based on genetic and phenotypic evidence, it is proposed that strain YIM 70179(T) represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia halophila sp. nov. is proposed. The type strain is YIM 70179(T) (=DSM 16378(T) =KCTC 19048(T)).
Li
Wen-Jun
WJ
The Key Laboratory for Microbial Resources of the Ministry of Education, PR China. wjli@ynu.edu.cn
Zhang
Yu-Qin
YQ
Schumann
Peter
P
Liu
Hong-Yu
HY
Yu
Li-Yan
LY
Zhang
Yue-Qin
YQ
Stackebrandt
Erko
E
Xu
Li-Hua
LH
Jiang
Cheng-Lin
CL
D015373:Bacterial Typing Techniques; D001483:Base Sequence; D002681:China; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis; D005227:Fatty Acids / Q000032:analysis; D020459:Genes, rRNA; D006863:Hydrogen-Ion Concentration; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride; D012987:Soil / Q000032:analysis; D012988:Soil Microbiology; D013045:Species Specificity
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D012987:Soil; D012965:Sodium Chloride