No association between tagging SNPs of SNARE complex genes (STX1A, VAMP2 and SNAP25) and schizophrenia in a Japanese population.
Abnormalities in neural connections and the neurotransmitter system appear to be involved in the pathophysiology of schizophrenia. The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex, which consists of Syntaxin1A, vesicle-associated membrane protein 2 (VAMP2) and synaptosomal-associated protein 25 kDa (SNAP25), plays an important role in the neurotransmitter system, and is therefore an attractive place to search for candidate genes for schizophrenia. We conducted a two-stage genetic association analysis of Syntaxin1A (STX1A), VAMP2 and SNAP25 genes with schizophrenia (first-set screening samples: 377 cases and 377 controls, second-set confirmation samples: 657 cases and 527 controls). Based on the linkage disequilibrium, 40 SNPs (STX1A, 8 SNPs; VAMP2, 3 SNPs; SNAP25, 29 SNPs) were selected as 'tagging SNPs'. Only nominally significant associations of an SNP (rs12626080) and haplotype (rs363014 and rs12626080) in SNAP25 were detected in the first-set screening scan. To validate this significance, we carried out a replication analysis of these SNP and haplotype associations in second-set samples with a denser set of markers (including five additional SNPs). However, these associations could not be confirmed in the second-set analysis. These results suggest that the SNARE complex-related genes do not play a major role in susceptibility to schizophrenia in the Japanese population.
Kawashima
Kunihiro
K
Department of Psychiatry, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.
Kishi
Taro
T
Ikeda
Masashi
M
Kitajima
Tsuyoshi
T
Yamanouchi
Yoshio
Y
Kinoshita
Yoko
Y
Takahashi
Nagahide
N
Saito
Shinichi
S
Ohi
Kazutaka
K
Yasuda
Yuka
Y
Hashimoto
Ryota
R
Takeda
Masatoshi
M
Inada
Toshiya
T
Ozaki
Norio
N
Iwata
Nakao
N
D000328:Adult; D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D020022:Genetic Predisposition to Disease; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D050600:SNARE Proteins / Q000235:genetics*; D012559:Schizophrenia / Q000235:genetics*; D050825:Synaptosomal-Associated Protein 25 / Q000235:genetics; D050827:Syntaxin 1 / Q000235:genetics; D050988:Vesicle-Associated Membrane Protein 2 / Q000235:genetics
D050600:SNARE Proteins; D050825:Synaptosomal-Associated Protein 25; D050827:Syntaxin 1; C494807:VAMP2 protein, human; D050988:Vesicle-Associated Membrane Protein 2