Radioprotection of tendon tissue via crosslinking and free radical scavenging.
Ionizing radiation could supplement tissue bank screening to further reduce the probability of diseases transmitted by allografts if denaturation effects can be minimized. It is important, however, such sterilization procedures be nondetrimental to tissues. We compared crosslinking and free radical scavenging potential methods to accomplish this task in tendon tissue. In addition, two forms of ionizing irradiation, gamma and electron beam (e-beam), were also compared. Crosslinkers included 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and glucose, which were used to add exogenous crosslinks to collagen. Free radical scavengers included mannitol, ascorbate, and riboflavin. Radioprotective effects were assessed through tensile testing and collagenase resistance testing after irradiation at 25 kGy and 50 kGy. Gamma and e-beam irradiation produced similar degenerative effects. Crosslinkers had the highest strength at 50 kGy, EDC treated tendons had 54% and 49% higher strength than untreated, for gamma and e-beam irradiation respectively. Free radical scavengers showed protective effects up to 25 kGy, especially for ascorbate and riboflavin. Crosslinked samples had higher resistance to collagenase and over a wider dose range than scavenger-treated. Of the options studied, the data suggest EDC precrosslinking or glucose treatment provides the best maintenance of native tendon properties after exposure to ionizing irradiation.
Orthopaedic Research Laboratories, Department of Orthopaedic Surgery, UMDNJ-Robert Wood Johnson Medical School, 51 French Street, Orthopaedic Surgery, Room 424, PO Box 19, New Brunswick, NJ 08903, USA.
Aaron
A
Seto
Charles J
CJ
Gatt
Michael G
MG
Dunn
D000818:Animals; D003094:Collagen / Q000187:drug effects / Q000528:radiation effects; D017364:Collagenases / Q000378:metabolism; D003432:Cross-Linking Reagents / Q000494:pharmacology*; D004307:Dose-Response Relationship, Radiation; D004583:Electrons; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000494:pharmacology; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology*; D005720:Gamma Rays / Q000009:adverse effects; D005947:Glucose / Q000494:pharmacology; D008297:Male; D011817:Rabbits; D011829:Radiation Dosage; D011835:Radiation Protection / Q000379:methods*; D011839:Radiation, Ionizing; D013242:Sterilization / Q000379:methods*; D013710:Tendons / Q000528:radiation effects*; D013718:Tensile Strength / Q000187:drug effects / Q000528:radiation effects; D014184:Transplantation, Homologous
D003432:Cross-Linking Reagents; D016166:Free Radical Scavengers; D003094:Collagen; D017364:Collagenases; D005947:Glucose; D005022:Ethyldimethylaminopropyl Carbodiimide