Effects of resistant starch type III polymorphs on human colon microbiota and short chain fatty acids in human gut models.
This study probed the possible effects of type III resistant starch (RS) crystalline polymorphism on RS fermentability by human gut microbiota and the short chain fatty acids production in vitro. Human fecal pH-controlled batch cultures showed RS induces an ecological shift in the colonic microbiota with polymorph B inducing Bifidobacterium spp. and polymorph A inducing Atopobium spp. Interestingly, polymorph B also induced higher butyrate production to levels of 0.79 mM. In addition, human gut simulation demonstrated that polymorph B promotes the growth of bifidobacteria in the proximal part of the colon and double their relative proportion in the microbiota in the distal colon. These findings suggest that RS polymorph B may promote large bowel health. While the findings are limited by study constraints, they do raise the possibility of using different thermal processing to delineate differences in the prebiotic capabilities of RS, especially its butryrogenicity in the human colon.
Lesmes
Uri
U
Faculty of Biotechnology & Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel.
Beards
Emma J
EJ
Gibson
Glenn R
GR
Tuohy
Kieran M
KM
Shimoni
Eyal
E
D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification / Q000378:metabolism*; D003106:Colon / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004040:Dietary Carbohydrates / Q000032:analysis / Q000378:metabolism*; D005232:Fatty Acids, Volatile / Q000378:metabolism*; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D006801:Humans; D008954:Models, Biological; D013213:Starch / Q000737:chemistry* / Q000378:metabolism*; D014961:X-Ray Diffraction
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D004040:Dietary Carbohydrates; D005232:Fatty Acids, Volatile; D013213:Starch