Autologous fat transplantation in the facial region--clinical and morphological studies of 30 cases.
OBJECTIVE
This study presents the results of a clinicomorphological study of autologous fat transplantation in the face region. The aim was to investigate and compare adipose tissue morphology after it was purified by two methods--centrifugation and serum lavage, and to find a correlation between the histological outcomes and the postoperative results.


METHODS
The evaluation was performed on a series of 30 patients assigned into two groups of 15 patients. Centrifuged adipose tissue was transplanted in one of the groups, and noncentrifuged (saline solution washed) in the other group. In all cases fat was obtained by syringe liposuction of subgluteal zones, processed by one of the two methods and then transplanted. Part of the material was fixed between vitaline membranes and embedded in paraffin. Histological analysis was performed after hematoxylin eosin staining of the slides. Post-operative effect was assessed by comparison of the pre- and postoperative photos on a 4-grade scale.


RESULTS
The morphological analysis revealed that adipose tissue was damaged by neither of the purification methods but the serum lavage preserved greater number of non-differentiated fat cells and connective tissue fragments. Furthermore, our clinical results suggested clearly that the noncentrifuged adipose tissue transplantation was more advantageous than the other type of transplantation.


CONCLUSIONS
The present study is an attempt to make a contribution towards a better understanding of the mechanisms involved in fat graft preservation.
Unit of Plastic and Craniofacial Surgery, St. George University Hospital, Plovdiv, Bulgaria.
Regina H
RH
Khater
Pepa K
PK
Atanassova
Yourii K
YK
Anastassov
D000273:Adipose Tissue / Q000637:transplantation*; D000293:Adolescent; D000328:Adult; D005145:Face / Q000601:surgery*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D013518:Surgery, Plastic / Q000379:methods*; D014182:Transplantation, Autologous