Stress-induced environmental changes in a single cell as revealed by fluorescence lifetime imaging.
The fluorescence lifetime image of HeLa cells expressing an enhanced green fluorescent protein (EGFP)-fusion protein changes under stress, which allows noninvasive determination of the status of individual cells.
Nakabayashi
Takakazu
T
Research Institute for Electronic Science, Hokkaido University, Sapporo, 060-0812, Japan.
Nagao
Issei
I
Kinjo
Masataka
M
Aoki
Yumiko
Y
Tanaka
Minoru
M
Ohta
Nobuhiro
N
D000818:Animals; D049452:Green Fluorescent Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D013997:Time Factors
C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins