Spindle disturbances in human-hamster hybrid (AL) cells induced by mobile communication frequency range signals.
The production of spindle disturbances in FC2 cells, a human-hamster hybrid (A(L)) cell line, by non-ionizing radiation was studied using an electromagnetic field with a field strength of 90 V/m at a frequency of 835 MHz. Due to the given experimental conditions slide flask cultures were exposed at room temperature in a microTEM (transversal electromagnetic field) cell, which allows optimal experimental conditions for small samples of biological material. Numerical calculations suggest that specific absorption rates of up to 60 mW/kg are reached for maximum field exposure. All exposure field parameters--either measured or calculable--are precisely defined and, for the first time, traceable to the standards of the SI system of physical units. Compared with co-incident negative controls, the results of two independently performed experiments suggest that exposure periods of time from 0.5 to 2 h with an electric field strength of 90 V/m are spindle acting agents as predominately indicated by the appearance of spindle disturbances at the ana- and telophase stages (especially lagging and non-disjunction of single chromosomes) of cell divisions. The spindle disturbances do not change the fraction of mitotic cells with increasing exposure time up to 2 h. Due to the applied experimental conditions an influence of temperature as a confounder parameter for spindle disturbances can be excluded.
Schrader
Thorsten
T
Physikalisch-Technische Bundesanstalt, Braunschweig, Germany. thorsten.schrader@ptb.de
Münter
Klaus
K
Kleine-Ostmann
Thomas
T
Schmid
Ernst
E
D000818:Animals; D002460:Cell Line; D040421:Cell Phone; D002869:Chromosome Aberrations / Q000528:radiation effects*; D006224:Cricetinae; D004307:Dose-Response Relationship, Radiation; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology* / Q000528:radiation effects*; D008872:Microwaves; D011829:Radiation Dosage; D008941:Spindle Apparatus / Q000528:radiation effects* / Q000648:ultrastructure*