Decrease in toll-like receptors 2 and 4 in the spleen of mouse with endotoxic tolerance.
OBJECTIVE
Down-regulation of toll-like receptor (TLRs) is present in animals with endotoxin tolerance. The spleen is the largest peripheral lymphatic organ in vivo, and it plays an important role in inflammatory reactivity. In this study we investigated TLR2 and TLR4 proteins in the spleen of endotoxic tolerant mice.


METHODS
The TLR2 and TLR4 proteins were quantitatively detected by Western blotting, and their locations in the spleen were observed by immunohistochemistry. IL1-beta mRNA in the spleen was analyzed by real-time quantitative PCR.


RESULTS
The mice pretreated with 0.5 mg/kg/day of LPS for 14 days exhibited a decrease of TLR2 and TLR4, and presented endotoxin tolerance. In addition, a bolus injection of 10 mg/kg LPS for mice with endotoxic tolerance caused no alteration of TLR2 and TLR4 proteins and no change of IL1-beta mRNA expression in the spleen.


CONCLUSIONS
TLR4 is the target of endotoxic tolerance induction. The induction of cross-tolerance of TLR2 following LPS challenge is present in vivo with endotoxin tolerance.
Zhong
B
B
Department of Pathogenic Biology, School of Medicine, Suzhou University, 199 Reng-al Roud, Dushu Lake Higher Eduction Town, Suzhou, Jiangsu 215123, PR China.
Ma
H Ying
HY
Yang
Q
Q
Gu
F Rong
FR
Yin
G Qing
GQ
Xia
C Ming
CM
D000818:Animals; D004731:Endotoxins / Q000276:immunology*; D006801:Humans; D007108:Immune Tolerance; D053583:Interleukin-1beta / Q000276:immunology; D008070:Lipopolysaccharides / Q000276:immunology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000166:cytology / Q000276:immunology*; D051195:Toll-Like Receptor 2 / Q000235:genetics / Q000276:immunology*; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology*
D004731:Endotoxins; D053583:Interleukin-1beta; D008070:Lipopolysaccharides; C493486:Tlr2 protein, mouse; C493487:Tlr4 protein, mouse; D051195:Toll-Like Receptor 2; D051197:Toll-Like Receptor 4