Uniform threshold intensity distribution-based quantitative multivariate imaging cytometry.
Implementation of quantitative analytical approaches to image-based cellular assays remains a major challenge. We disclose a tool to achieve automatic rapid quantitative cellular imaging analysis based on uniform threshold intensity distribution. An acousto-optic tunable filter-based, quantitative multivariate imaging cytometer was set up to elucidate drug-induced cell death dynamics via cell viability and apoptosis/necrosis measurements in the human myeloid leukemia cell line, HL-60. Cells were treated with various drugs (camptothecin, naringenin, sodium salicylate) at different concentrations and time intervals. The developed protocol can directly depict and quantitate targeted cellular moieties, subsequently enabling a method that is applicable to various cellular assays with special reference to next generation drug discovery screening. This may also complement certain flow-cytometric measurements in studying quantitative physiology of cellular systems.
Naoghare
Pravin K
PK
Research Institute of Pharmaceutical Sciences and College of Pharmacy, Seoul National University, Seoul 151-742, South Korea.
Kim
Min Jung
MJ
Song
Joon Myong
JM
D002470:Cell Survival; D018922:HL-60 Cells; D006801:Humans; D019044:Image Cytometry / Q000295:instrumentation* / Q000379:methods*