The development of point-of-use water filters as sampling devices in bioforensics: extent of microbial sorption and elution.
The foundational idea for this project is that household faucet-mounted water filters may be used as bioforensic sampling devices to detect the extent of a potential bioagent release in domestic water supplies. An optimized eluent solution was determined experimentally by quantifying recoveries of microorganisms from point-of-use (POU) drinking water filters. The optimized extraction protocol was then used in mock bioagent release experiments to determine the feasibility of POU filters as bioforensic sampling devices. Bacillus atrophaeus spores, Escherichia coli and PP7 virus were exposed to filters and the number of attached organisms was determined by enumerating the unattached organisms on selective agar media. Subsequently, the filters were eluted and the percent of extracted organisms was determined based on the number of attached organisms. Two popular brands of carbon block filters retained 92%-99% of representative virus, spore and vegetative bacteria. In back-flush elutions of single filters, the most efficient eluent was identified as a combination of 1% peptone and 1% Tween-80, and extraction recovered 25.4% (+/-17.5%) of attached E. coli, 20.4% (+/-3.6%) of B. atrophaeus spores, and 9.4% (+/-5.2%) of PP7 virions (+/- standard deviations). In bioagent release studies in which filters were challenged with 100 agents mL(-1), greater than 99% of the spores were retained by the filters, and the percent of attached spores that were recovered ranged from 10.4% at day 0 to 4.3% five days after the release event (averaged from five separate experiments). In contrast, E. coli, Salmonella typhimurium and PP7 virus were rapidly inactivated in the chlorinated tap water, indicating their improbable survival in chlorinated water supplies. It is therefore concluded that household water filters can be used as microbial sampling devices for bioforensic applications in the event of a bioagent release in domestic drinking water supplies.
Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Jennifer L
JL
Sedillo
Ayshea
A
Quintana
Kathryn
K
Souza
Kevin H
KH
Oshima
Geoffrey B
GB
Smith
D000327:Adsorption; D000818:Animals; D001407:Bacillus / Q000302:isolation & purification; D001419:Bacteria / Q000302:isolation & purification*; D001435:Bacteriophages / Q000302:isolation & purification; D015169:Colony Count, Microbial; D004926:Escherichia coli / Q000302:isolation & purification; D005374:Filtration / Q000295:instrumentation / Q000379:methods*; D006801:Humans; D018570:Risk Assessment; D012486:Salmonella typhimurium / Q000302:isolation & purification; D014871:Water Microbiology; D018508:Water Purification / Q000295:instrumentation / Q000379:methods*; D014881:Water Supply