[Role of caveolin-1 down-regulation by iRNA in human hepatocyte proliferation].
OBJECTIVE
To investigate the role of caveolin-1 down-regulation in human hepatocyte proliferation in vitro.


METHODS
The expression vector psiRNA-CAV1 was constructed and transfected into Chang liver cells (CHL). The caveolin-1 down-regulated cell clones were selected by the antibiotic zeocin. The proliferation of the cell strain CAV7 was examined by MTT, in which untransfected CHL and HepG2 cells were set as controls. Expression of caveolin-1, Akt, Erk1/2, p-Akt and p-Erk1/2 in the transfected and control cells was detected by Western blot.


RESULTS
After caveolin-1 expression was down-regulated by RNAi, CHL increased faster at first (24 h and 72 h, P<0.05; 96 h, P<0.01), but slower later. P-Akt and p-Erk1/2 expressions were down-regulated, indicating that the growth and proliferation related Akt and Erk1/2 pathways were inhibited after caveolin-1 down-regulation.


CONCLUSIONS
Caveolin-1 may play an important role in hepatocyte proliferation.
College of Life Sciences, Analysis and Testing Center, Liaoning Normal University, Dalian 116029, China.
Gang
G
Ren
Ying
Y
Liu
Xiao-min
XM
Wang
Chun-hui
CH
Zhao
Wei
W
Zou
D051242:Caveolin 1 / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D015536:Down-Regulation; D022781:Hepatocytes / Q000166:cytology*; D006801:Humans; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D034622:RNA Interference; D034741:RNA, Small Interfering; D015398:Signal Transduction; D014162:Transfection
C495540:CAV1 protein, human; D051242:Caveolin 1; D034741:RNA, Small Interfering; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt