A mouse plasma peptide atlas as a resource for disease proteomics.
We present an in-depth analysis of mouse plasma leading to the development of a publicly available repository composed of 568 liquid chromatography-tandem mass spectrometry runs. A total of 13,779 distinct peptides have been identified with high confidence. The corresponding approximately 3,000 proteins are estimated to span a 7 logarithmic range of abundance in plasma. A major finding from this study is the identification of novel isoforms and transcript variants not previously predicted from genome analysis.
Zhang
Qing
Q
Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA. qing@fhcrc.org
Menon
Rajasree
R
Deutsch
Eric W
EW
Pitteri
Sharon J
SJ
Faca
Vitor M
VM
Wang
Hong
H
Newcomb
Lisa F
LF
Depinho
Ronald A
RA
Bardeesy
Nabeel
N
Dinulescu
Daniela
D
Hung
Kenneth E
KE
Kucherlapati
Raju
R
Jacks
Tyler
T
Politi
Katerina
K
Aebersold
Ruedi
R
Omenn
Gilbert S
GS
States
David J
DJ
Hanash
Samir M
SM
D017398:Alternative Splicing; D000818:Animals; D030562:Databases, Protein; D006801:Humans; D051379:Mice; D010455:Peptides / Q000032:analysis*; D010949:Plasma / Q000737:chemistry*; D020033:Protein Isoforms; D040901:Proteomics; D053719:Tandem Mass Spectrometry
D010455:Peptides; D020033:Protein Isoforms