Biofuel cell system employing thermostable glucose dehydrogenase.
Enzyme biofuel cells utilizing glucose dehydrogenase as an anode enzyme were constructed. The glucose dehydrogenase is composed of a catalytic subunit, an electron transfer subunit, and a chaperon-like subunit. Cells, constructed using either a glucose dehydrogenase catalytic subunit or a glucose dehydrogenase complex, displayed power outputs that were dependent on the glucose concentration. The catalytic subunit in the anode maintained its catalytic activity for 24 h of operation. The biofuel cell which composed of glucose dehydrogenase complex functioned successfully even in the absence of an electron mediator at the anode cell. These results indicate the potential application of this thermostable glucose dehydrogenase for the construction of a compartment-less biofuel cell.
Biometerials center, National Institute for Materials Science (NIMS), 2-24-16 Nakamachi, Koganei, Tokyo 184-8588, Japan.
Junko
J
Okuda-Shimazaki
Noriko
N
Kakehi
Tomohiko
T
Yamazaki
Masamitsu
M
Tomiyama
Koji
K
Sode
D001674:Bioelectric Energy Sources; D016956:Burkholderia cepacia / Q000235:genetics; D002384:Catalysis; D004795:Enzyme Stability; D042843:Glucose 1-Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D011994:Recombinant Proteins; D042843:Glucose 1-Dehydrogenase