[Determination of five primary chemical constituents in Cassia angustifolia by HPLC].
OBJECTIVE
To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia.


METHODS
The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (10:90-15: 85-18: 82-20: 80-25: 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm.


RESULTS
The results showed that 5 effective components all separated well and showed good linearity.


CONCLUSIONS
The method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.
Wu
Qiu-Ping
QP
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang
Zhu-Ju
ZJ
Tang
Li-Ying
LY
Fu
Mei-Hong
MH
He
Yan
Y
Fang
Jing
J
Gong
Qian-Feng
QF
D000880:Anthraquinones / Q000737:chemistry*; D047310:Apigenin / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005960:Glucosides / Q000737:chemistry*; D009281:Naphthalenes / Q000737:chemistry*; D015203:Reproducibility of Results; D012676:Senna Extract; D029846:Senna Plant / Q000737:chemistry*; D000081226:Sennosides; D013696:Temperature
D000880:Anthraquinones; D005960:Glucosides; D009281:Naphthalenes; D000081226:Sennosides; C530449:apigenin-6,8-di-C-glycopyranoside; C459627:tinnevellin 8-O-glucoside; D047310:Apigenin; D012676:Senna Extract