Proteomic and molecular investigation on the physiological adaptation of Comamonas sp. strain CNB-1 growing on 4-chloronitrobenzene.
Comamonas sp. strain CNB-1 can utilize 4-chloronitrobenzene (4CNB) as sole carbon and nitrogen source for growth. Previous studies were focused on 4CNB degradative pathway and have showed that CNB-1 contained a plasmid pCNB1 harboring the genes (cnbABCaCbDEFGH, cnbZ) for the enzymes involving in 4CNB degradation, but only three gene products (CnbCa, CnbCb, and CnbZ) were identified in CNB-1 cells. Comamonas strain CNB-2 that lost pCNB1 was not able to grow on 4CNB. In this study, physiological adaptation to 4CNB by CNB-1 was investigated with proteomic and molecular tools. Comparative proteomes of strains CNB-1 and CNB-2 grown on 4CNB and/or succinate revealed that adaptation to 4CNB by CNB-1 included specific degradative pathway and general physiological responses: (1) Seven gene products (CnbA, CnbCa, CnbCb, CnbD, CnbE, CnbF, and CnbZ) for 4CNB degradation were identified in 4CNB-grown cells, and they were constitutively synthesized in CNB-1. Two genes cnbE and cnbF were cloned and simultaneously expressed in E. coli. The CnbE and CnbF together catalyzed the conversion of 2-oxohex-4-ene-5-chloro-1,6-dioate into 2-oxo-4-hydroxy-5-chloro-valeric acid; (2) Enzymes involving in glycolysis, tricarboxylic acid cycle, and synthesis of glutamate increased their abundances in 4CNB-grown cells.
State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Sciences, Chaoyang, Beijing, People's Republic of China.
Yun
Y
Zhang
Jian-Feng
JF
Wu
Josef
J
Zeyer
Bo
B
Meng
Lei
L
Liu
Cheng-Ying
CY
Jiang
Si-Qi
SQ
Liu
Shuang-Jiang
SJ
Liu
D000222:Adaptation, Physiological; D001426:Bacterial Proteins / Q000378:metabolism*; D020585:Comamonas / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D013058:Mass Spectrometry; D009578:Nitrobenzenes / Q000378:metabolism*; D040901:Proteomics; D019802:Succinic Acid / Q000378:metabolism
D001426:Bacterial Proteins; D009578:Nitrobenzenes; D019802:Succinic Acid; C010407:4-chloronitrobenzene