Coating didodecyldimethylammonium bromide onto Au nanoparticles increases the stability of its complex with DNA.
The stability of the complex of cationic lipid with nucleic acid, especially when facing serum, is crucial for the efficiency of gene delivery. Here, we demonstrated that the stability of the complex of didodecyldimethylammonium bromide (DDAB, a cationic lipid) with DNA in the presence of serum dramatically increased after coating DDAB onto the surface of the gold nanoparticles. The stability of the complex was demonstrated with dye intercalation assay, and agarose gel electrophoresis. The process of the interaction was characterized with UV-vis spectra and the morphology of the complex was observed with atomic force microscope (AFM). Cell viability assays demonstrated that the cytotoxicity of DDAB was also decreased.
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun, Jilin 130022, PR China.
Peicai
P
Li
Lixue
L
Zhang
Kelong
K
Ai
Dan
D
Li
Xiaohua
X
Liu
Erkang
E
Wang
D001798:Blood Proteins / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D004587:Electrophoresis, Agar Gel; D018014:Gene Transfer Techniques; D006046:Gold / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D006801:Humans; D053768:Metal Nanoparticles; D018625:Microscopy, Atomic Force; D008956:Models, Chemical; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D000644:Quaternary Ammonium Compounds / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties
D001798:Blood Proteins; D000644:Quaternary Ammonium Compounds; C046112:didodecyldimethylammonium; D006046:Gold; D004247:DNA