Oxylipin and mitochondrion probes to track yeast sexual cells.
When oxylipin and mitochondrion probes, i.e., fluorescing antibodies specific for 3-hydroxy fatty acids (3-OH oxylipins) and rhodamine 123 (Rh123), were added to yeast cells, these probes accumulated mainly in the sexual cells (i.e., both associated with ascospores) and not in the vegetative cells. This suggests increased mitochondrial activity in asci, since 3-OH oxylipins are mitochondrially produced and it is known that Rh123 accumulates selectively in functional mitochondria that maintain a high transmembrane potential (Delta Psi m). This increased activity may be necessary for the production and effective release of the many spores found in single-celled asci. These results may be useful in the rapid identification of asci and in yeast sexual spore mechanics, which may find application in yeast systematics as well as hydro-, aero-, and nano-technologies.
UNESCO MIRCEN Industrial Biotechnology, Department of Microbial, Biochemical and Food Biotechnology, University of the Free State, Bloemfontein, South Africa.
Desmond M
DM
Ncango
Chantel W
CW
Swart
Monique E
ME
Goldblatt
Carolina H
CH
Pohl
Pieter W J
PW
Van Wyk
Piet J
PJ
Botes
Johan L F
JL
Kock
D003592:Cytophotometry / Q000379:methods*; D005456:Fluorescent Dyes / Q000032:analysis / Q000378:metabolism; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000737:chemistry / Q000378:metabolism; D015335:Molecular Probes / Q000032:analysis* / Q000378:metabolism; D054883:Oxylipins / Q000032:analysis* / Q000378:metabolism; D020112:Rhodamine 123 / Q000032:analysis / Q000378:metabolism; D004718:Saccharomycetales / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism; D013172:Spores, Fungal / Q000737:chemistry / Q000166:cytology* / Q000378:metabolism
D005456:Fluorescent Dyes; D015335:Molecular Probes; D054883:Oxylipins; D020112:Rhodamine 123