Imup-1 and imup-2 overexpression in endometrial carcinoma in Korean and Japanese populations.
BACKGROUND
Endometrial carcinoma is a well known complex gynecological disorder. Our team suggests that this tumor is related to immortalization-up-regulated protein 1 and 2 (imup-1, imup-2), which are known to be involved in SV40-mediated immortalization.


METHODS
RT-PCR and immunohistochemical staining were used to examine the mRNA expression levels and intracellular localization of imup-1 and imup-2 in endometrial carcinomas from Korean and Japanese patients.


RESULTS
The imup-1 (4.1- and 23.6-fold) and imup-2 (4.8- and 2.7-fold) mRNA expression levels in endometrial carcinomas from both Korean and Japanese women were significantly higher than in normal endometrial tissues (p < 0.01). Strong expression of the IMUP-1 and IMUP-2 proteins were found in the tumor cytoplasm as well as in the nuclei.


CONCLUSIONS
These findings demonstrate the up-regulation of imup-1 and imup-2 in human endometrial carcinomas and indicate that these molecules play a role in endometrial carcinogenesis in both Korean and Japanese patients.
Department of Obstetrics and Gynecology, College of Medicine, Pochon CHA University, Sungnam, Korea.
Seung Jo
SJ
Kim
Hee Jung
HJ
An
Hyo Jin
HJ
Kim
Hyun Min
HM
Jungs
Sunyoung
S
Lee
Jung Jae
JJ
Ko
In Ho
IH
Kim
Noriaki
N
Sakuragi
Jin Kyeoung
JK
Kim
D016889:Endometrial Neoplasms / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007564:Japan; D007723:Korea; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
C439765:C19orf33 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D014157:Transcription Factors