Leishmania mexicana: participation of NF-kappaB in the differential production of IL-12 in dendritic cells and monocytes induced by lipophosphoglycan (LPG).
Dendritic cells (DC) and macrophages (Mphi) are well known as important effectors of the innate immune system and their ability to produce IL-12 indicates that they possess the potential of directing acquired immunity toward a Th1-biased response. Interestingly, the intracellular parasite Leishmania has been shown to selectively suppress Mphi IL-12 production and are DC the principal source of this cytokine. The molecular details of this phenomenon remain enigmatic. In the present study we examined the effect of Leishmania mexicana lipophosphoglycan (LPG) on the production of IL-12, TNF-alpha, and IL-10 and nuclear translocation of NF-kappaB. The results show that LPG induced more IL-12 in human DC than in monocytes. This difference was due in part to nuclear translocation of NF-kappaB, since LPG induced more translocation in DC than in monocytes. These results suggest that Leishmania LPG impairs nuclear translocation of NF-kappaB in monocytes with the subsequent decrease in IL-12 production.
Departamento de Medicina Experimental, Facultad de Medicina, Universidad Nacional Autónoma de México, Dr. Balmis 148, Col. Doctores, México 06726, Mexico.
Jesús
J
Argueta-Donohué
Nuria
N
Carrillo
Leonardo
L
Valdés-Reyes
Alejandro
A
Zentella
Magdalena
M
Aguirre-García
Ingeborg
I
Becker
Laila
L
Gutiérrez-Kobeh
D000818:Animals; D002478:Cells, Cultured; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology* / Q000469:parasitology; D006028:Glycosphingolipids / Q000494:pharmacology*; D006801:Humans; D016753:Interleukin-10 / Q000096:biosynthesis; D018664:Interleukin-12 / Q000032:analysis / Q000096:biosynthesis*; D007894:Leishmania mexicana / Q000737:chemistry / Q000276:immunology / Q000502:physiology*; D009000:Monocytes / Q000187:drug effects / Q000276:immunology* / Q000469:parasitology; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis
D006028:Glycosphingolipids; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; C008290:lipophosphonoglycan; D016753:Interleukin-10; D018664:Interleukin-12