In situ activation of antigen-specific CD8+ T cells in the presence of antigen in organotypic brain slices.
The activation of Ag-specific T cells locally in the CNS could potentially contribute to the development of immune-mediated brain diseases. We addressed whether Ag-specific T cells could be stimulated in the CNS in the absence of peripheral lymphoid tissues by analyzing Ag-specific T cell responses in organotypic brain slice cultures. Organotypic brain slice cultures were established 1 h after intracerebral OVA Ag microinjection. We showed that when OVA-specific CD8(+) T cells were added to Ag-containing brain slices, these cells became activated and migrated into the brain to the sites of their specific Ags. This activation of OVA-specific T cells was abrogated by the deletion of CD11c(+) cells from the brain slices of the donor mice. These data suggest that brain-resident CD11c(+) cells stimulate Ag-specific naive CD8(+) T cells locally in the CNS and may contribute to immune responses in the brain.
Ling
Changying
C
Department of Pathology and Laboratory Medicine, University of Wisconsin Medical School, University of Wisconsin, Madison, WI 53706, USA.
Verbny
Yakov I
YI
Banks
Matthew I
MI
Sandor
Matyas
M
Fabry
Zsuzsanna
Z
D000818:Animals; D001921:Brain / Q000166:cytology* / Q000276:immunology* / Q000378:metabolism; D039521:CD11c Antigen / Q000096:biosynthesis; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D049109:Cell Proliferation; D002474:Cell-Free System / Q000276:immunology / Q000378:metabolism; D018920:Coculture Techniques; D018984:Epitopes, T-Lymphocyte / Q000096:biosynthesis / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D009924:Organ Culture Techniques; D009928:Organ Specificity / Q000276:immunology; D010047:Ovalbumin / Q000276:immunology
D039521:CD11c Antigen; D018984:Epitopes, T-Lymphocyte; D010047:Ovalbumin