Characterization of a carbonyl-conjugated polyene precursor in 10-membered enediyne biosynthesis.
We have characterized a linear carbonyl-conjugated polyene generated by the iterative polyketide synthase (CalE8) involved in the biosynthesis of the 10-membered enediyne core of calicheamicin. The results provide insight into the mysterious biosynthetic mechanism of the unique enediyne. The carbonyl-conjugated polyene differs from the precursor for 9-membered enediyne, suggesting that the divergence of enediyne biosynthesis starts at the PKS stage.
School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551.
Rong
R
Kong
Lan Pei
LP
Goh
Chong Wai
CW
Liew
Qin Shi
QS
Ho
Elavazhagan
E
Murugan
Bin
B
Li
Kai
K
Tang
Zhao-Xun
ZX
Liang
D000617:Aminoglycosides / Q000096:biosynthesis / Q000737:chemistry; D053281:Enediynes / Q000138:chemical synthesis* / Q000737:chemistry; D008316:Malonyl Coenzyme A; D011090:Polyenes; D048630:Polyketide Synthases / Q000378:metabolism*
D000617:Aminoglycosides; D053281:Enediynes; D011090:Polyenes; C489066:calicheamicin enediyne; D008316:Malonyl Coenzyme A; D048630:Polyketide Synthases