Effects of phenol in antioxidant metabolism in matrinxã, Brycon amazonicus (Teleostei; Characidae).
Parameters of the antioxidant defense systems of Brycon amazonicus (matrinxã--a neotropical fish) exposed to phenol for 96 h plus the recovery over 1 and 2 weeks were studied in erythrocytes and liver. Hematocrit increase was observed during phenol exposure and recovery for 1 week. Total superoxide dismutases (SOD), glutathione peroxidase (GPx) and reduced glutathione (GSH) did not change during phenol exposure. Erythrocyte glucose-6-phosphate dehydrogenase (G6PDH) increased during that period while catalase (CAT) activity decreased during phenol exposure and recovery for 2 weeks. In the liver, SOD and CAT did not change, whereas GPx increased in the first week of recovery and decreased after 2 weeks. A late response was observed for G6PDH activity which increased only at the second week. Ascorbate concentration in the brain decreased during phenol exposure and increased over recovery. From our results it appears that the oxidative stress was limited in matrinxã exposed to phenol, but seemed to occur during the recovery period.
Department of Genetics and Evolution, Federal University of Sao Carlos, (UFSCar), SP, Brazil.
Ive Marchioni
IM
Avilez
Tiago Silvestre Fernandes
TS
Hori
Luciana Cristina
LC
de Almeida
Araceli
A
Hackbarth
Jayme
J
da Cunha Bastos Neto
Vera Lucia Freire
VL
da Cunha Bastos
Gilberto
G
Moraes
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D001205:Ascorbic Acid / Q000378:metabolism; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D004912:Erythrocytes / Q000187:drug effects* / Q000201:enzymology; D005399:Fishes / Q000097:blood / Q000378:metabolism*; D005954:Glucosephosphate Dehydrogenase / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D006400:Hematocrit; D008099:Liver / Q000187:drug effects* / Q000201:enzymology; D018384:Oxidative Stress / Q000187:drug effects*; D019800:Phenol / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D000975:Antioxidants; D014874:Water Pollutants, Chemical; D019800:Phenol; D005954:Glucosephosphate Dehydrogenase; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D001205:Ascorbic Acid