Production and characterization of the exopolysaccharides produced by Agaricus brasiliensis in submerged fermentation.
The aim of the work was to study the production of the exopolysaccharides by Agaricus brasiliensis and the isolation of exopolysaccharides (EPSs) with biological effects. A brasiliensis LPB03 was cultured in submerged fermentation in a medium containing glucose, yeast extract, hydrolyzed soybean protein, and salts (pH 6.1) at 29 degrees C and 120 rpm for 144 h. The maximum biomass and EPS yield was 7.80 +/- 0.01 and 1,430.70 +/- 26.75 mg/L, respectively. To isolate the produced EPSs, two methods were compared: (1) with alcohol precipitation and (2) treatment with tricloroacetic acid (TCA), followed by alcohol precipitation. The use of TCA facilitated the purification of the EPS, reducing the amount of the contaminant soy proteins. For monosaccharide identification, the EPSs were hydrolyzed, derivatized to alditol acetates, and analyzed by gas chromatography (GC) and GC-mass spectrometry, which showed the presence (in molar percentage) of mannose (58.7), galactose (21.4), and glucose (13.1) as major sugars, with lower amounts of rhamnose (3.9) and xylose (2.8). Scanning electron microscopy was used to observe the morphological structure of the EPS. The experiments in vivo including EPS in the mice diet during 8 weeks indicated the hipocholesteremic and hypoglycemic effects.
Lima
L F O
LF
Department of Chemical Engineering, Bioprocess Engineering and Biotechnology Division, Federal University of Paraná, Curitiba, PR, Brazil.
Habu
S
S
Gern
J C
JC
Nascimento
B M
BM
Parada
Jose-Luis
JL
Noseda
M D
MD
Gonçalves
A G
AG
Nisha
V R
VR
Pandey
Ashok
A
Soccol
Vanete Thomaz
VT
Soccol
Carlos R
CR
D000364:Agaricus / Q000254:growth & development / Q000378:metabolism*; D000818:Animals; D000924:Anticholesteremic Agents / Q000494:pharmacology; D018533:Biomass; D001786:Blood Glucose / Q000378:metabolism; D002784:Cholesterol / Q000097:blood; D003470:Culture Media; D000431:Ethanol; D005260:Female; D005285:Fermentation; D005590:Fractional Precipitation; D007004:Hypoglycemic Agents / Q000494:pharmacology; D051379:Mice; D008854:Microscopy, Electron; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D030262:Soybean Proteins; D014238:Trichloroacetic Acid
D000924:Anticholesteremic Agents; D001786:Blood Glucose; D003470:Culture Media; D007004:Hypoglycemic Agents; D011134:Polysaccharides; D030262:Soybean Proteins; D000431:Ethanol; D014238:Trichloroacetic Acid; D002784:Cholesterol