Directed motion of proteins along tethered polyelectrolytes.
We present the first time-resolved investigation of motions of proteins in densely grafted layers of spherical polyelectrolyte brushes. Using small-angle x-ray scattering combined with rapid stopped-flow mixing, we followed the uptake of bovine serum albumin by poly(acrylic acid) layer with high spatial and temporal resolution. We find that the total amount of adsorbed protein scales with time as t(1/4). This subdiffusive behavior is explained on the basis of directed motion of the protein along the polyelectrolyte chains.
Physikalische Chemie I, University of Bayreuth, Bayreuth, Germany.
Katja
K
Henzler
Sabine
S
Rosenfeldt
Alexander
A
Wittemann
Ludger
L
Harnau
Stephanie
S
Finet
Theyencheri
T
Narayanan
Matthias
M
Ballauff
D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D007700:Kinetics; D009994:Osmolar Concentration; D011137:Polystyrenes / Q000737:chemistry*; D053838:Scattering, Small Angle; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D014961:X-Ray Diffraction / Q000379:methods
D000180:Acrylic Resins; D011137:Polystyrenes; D012710:Serum Albumin, Bovine; C006903:carbopol 940