Beta1 integrins regulate chondrogenesis and rock signaling in adipose stem cells.
Beta1 integrins play a controversial role during chondrogenesis. Since the maturation of chondrocytes relies on a signaling switch from cell-cell to cell-matrix interactions, we hypothesized that beta1 integrins play a different role at the earlier (mainly cell-cell interaction) from the later stage (mainly cell-matrix interaction) of chondrogenesis. Our data showed: in plain medium, sox9, collagen X, and collagen II gene expressions of ASCs were induced by beta1-integrin blockage at day 14. In chondrogenic medium, however, sox 9, sox6, and collagen II gene expression were induced at day 4 but inhibited at day 14. In addition, both beta1-integrin blockage and TGF-beta1 down-regulated Rock-1 and -2 gene expression and produced the round cells. We concluded that beta1 integrins play a more important role at the later stages than earlier stages of chondrogenesis, and that the onset of chondrogenesis promoted by beta1-integrin blockage might be through inhibiting Rock signaling.
Department of Orthopaedic Surgery, VU University Medical Center, Amsterdam, The Netherlands.
Z F
ZF
Lu
B
B
Zandieh Doulabi
C L
CL
Huang
R A
RA
Bank
M N
MN
Helder
D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects; D048430:Cell Shape / Q000235:genetics; D020219:Chondrogenesis / Q000235:genetics; D024043:Collagen Type II / Q000235:genetics; D024121:Collagen Type X / Q000235:genetics; D003470:Culture Media; D004268:DNA-Binding Proteins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D018507:Gene Expression Regulation, Developmental; D006609:High Mobility Group Proteins / Q000235:genetics; D006801:Humans; D007074:Immunoglobulin G / Q000494:pharmacology; D019012:Integrin beta1 / Q000494:pharmacology / Q000502:physiology*; D055758:SOX9 Transcription Factor; D055755:SOXD Transcription Factors; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics; D053773:Transforming Growth Factor beta1 / Q000037:antagonists & inhibitors / Q000494:pharmacology / Q000502:physiology; D054460:rho-Associated Kinases / Q000235:genetics*
D024043:Collagen Type II; D024121:Collagen Type X; D003470:Culture Media; D004268:DNA-Binding Proteins; D006609:High Mobility Group Proteins; D007074:Immunoglobulin G; D019012:Integrin beta1; C486270:SOX6 protein, human; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D055755:SOXD Transcription Factors; D014157:Transcription Factors; D053773:Transforming Growth Factor beta1; D054460:rho-Associated Kinases