Amplifying small amounts of tumor DNA allows detection of DNA copy number abberations with array-CGH.
Array comparative genomic hybridization (aCGH) is a powerful tool to detect relative DNA copy number at a resolution limited only by the coverage of bacterial artificial chromosomes (BACs) used to print the genomic array. The amount of DNA needed to perform a reliable aCGH analysis has been a limiting factor, especially on minute tissue samples where limited DNA is available. Here we report a simple, highly sensitive and reliable aCGH method to analyze samples of no more than 1 ng genomic DNA. The speed and simplicity of the technique are ideal for studies on small clinical samples such as needle biopsies.
Prestegarden
Lars
L
NorLux Neuro Oncology, Department of Biomedicine, University of Bergen, Bergen, Norway. lars.prestegarden@student.uib.no
Misra
Anjan
A
Ware
Marcus L
ML
Yeh
Ru-Fang
RF
Bjerkvig
Rolf
R
Feuerstein
Burt G
BG
D045744:Cell Line, Tumor; D004273:DNA, Neoplasm / Q000032:analysis*; D018628:Gene Dosage; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods*; D020411:Oligonucleotide Array Sequence Analysis
D004273:DNA, Neoplasm