NMR study of cellulose and wheat straw degradation by Ruminococcus albus 20.
Cellulose and wheat straw degradation by Ruminococcus albus was monitored using NMR spectroscopy. In situ solid-state (13)C-cross-polarization magic angle spinning NMR was used to monitor the modification of the composition and structure of cellulose and (13)C-enriched wheat straw during the growth of the bacterium on these substrates. In cellulose, amorphous regions were not preferentially degraded relative to crystalline areas by R. albus. Cellulose and hemicelluloses were also degraded at the same rate in wheat straw. Liquid state two-dimensional NMR experiments were used to analyse in detail the sugars released in the culture medium, and the integration of NMR signals enabled their quantification at various times of culture. The results showed glucose and cellodextrin accumulation in the medium of cellulose cultures; the cellodextrins were mainly cellotriose and accumulated to up to 2 mm after 4 days. In the wheat straw cultures, xylose was the main soluble sugar detected (1.4 mm); arabinose and glucose were also found, together with some oligosaccharides liberated from hemicellulose hydrolysis, but to a much lesser extent. No cellodextrins were detected. The results indicate that this strain of R. albus is unable to use glucose, xylose and arabinose for growth, but utilizes efficiently xylooligosaccharides. R. albus 20 appears to be less efficient than Fibrobacter succinogenes S85 for the degradation of wheat straw.
Institute of Chemistry, Slovak Academy of Sciences, Centre for Glycomics, Bratislava, Slovak Republic.
Maria
M
Matulova
Régis
R
Nouaille
Peter
P
Capek
Michel
M
Péan
Anne-Marie
AM
Delort
Evelyne
E
Forano
D001089:Arabinose / Q000737:chemistry; D002482:Cellulose / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism; D003912:Dextrins / Q000737:chemistry; D005285:Fermentation; D045848:Fibrobacter / Q000378:metabolism; D005947:Glucose / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D045857:Ruminococcus / Q000378:metabolism*; D013696:Temperature; D013997:Time Factors; D014908:Triticum / Q000737:chemistry* / Q000378:metabolism; D014994:Xylose / Q000737:chemistry
D003912:Dextrins; D002482:Cellulose; C068994:cellodextrin; D014994:Xylose; D001089:Arabinose; D005947:Glucose