Microcystin-induced oxidative stress in Laeonereis acuta (Polychaeta, Nereididae).
Oxidative stress induced by microcystins was evaluated in an estuarine worm, Laeonereis acuta (Nereididae). Ten organisms were exposed to lyophilized cells of a toxic Microcystisaeruginosa strain RST9501 ( approximately 2 microg/mL microcystins, MC); 10 were exposed to lyophilized cells of a nontoxic Aphanotece sp. strain RSMan92 and 10 were maintained without cyanobacterial cells. Exposure time was 48 h. The enzymatic antioxidant defenses, as well as the oxidative damage, were analyzed. Toxic and nontoxic cyanobacteria lowered catalase activity with no changes in glutathione reductase and glutathione-S-transferase activities. This may have led to toxin intracellular accumulation, which should favor oxidative stress generation, observed by the high lipid peroxide and DNA-protein crosslink levels in the group exposed to MC.
Leão
J C
JC
Unidade de Pesquisa em Cianobactérias, Fundação Universidade Federal do Rio Grande, Pós-Graduação em Oceanografia Biológica, Rio Grande/RS, Brazil.
Geracitano
L A
LA
Monserrat
J M
JM
Amado
L L
LL
Yunes
J S
JS
D000818:Animals; D008387:Marine Toxins / Q000633:toxicity*; D052998:Microcystins / Q000633:toxicity*; D046931:Microcystis / Q000737:chemistry; D018384:Oxidative Stress / Q000187:drug effects*; D011077:Polychaeta / Q000187:drug effects* / Q000201:enzymology
D008387:Marine Toxins; D052998:Microcystins