Isoform-specific interaction of pyruvate kinase with hepatitis C virus NS5B.
Hepatitis C virus (HCV) replication and pathogenesis involve both virus-encoded proteins and cellular factors. In our study, we showed that NS5B, the HCV RNA-dependent RNA polymerase, interacted with M2 type pyruvate kinase (M2PK) but not L type pyruvate kinase. We confirmed the interaction by GST pull down, coimmunoprecipitation and confocal immunofluorescence analysis in cells with transient expression of NS5B and M2PK as well as in a HCV replicon-bearing cell line. Furthermore shRNA which specifically down-regulated M2PK expression could inhibit the replication of HCV in HCV replicon 9B cells.
Wu
Xiaoyun
X
State Key Laboratory of Virology, the Modern Virology Research Center, College of Life Sciences, Wuhan University, Wuhan, PR China.
Zhou
You
Y
Zhang
Ke
K
Liu
Qingzhen
Q
Guo
Deyin
D
D000818:Animals; D002460:Cell Line; D016679:Genome, Viral; D016174:Hepacivirus / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007527:Isoenzymes / Q000378:metabolism; D051379:Mice; D011770:Pyruvate Kinase / Q000235:genetics / Q000378:metabolism*; D012093:Replicon; D020798:Two-Hybrid System Techniques; D017361:Viral Nonstructural Proteins / Q000378:metabolism*; D012324:RNA-Dependent RNA Polymerase
D007527:Isoenzymes; D011770:Pyruvate Kinase; D017361:Viral Nonstructural Proteins; C087036:NS-5 protein, hepatitis C virus; D012324:RNA-Dependent RNA Polymerase