Chemokine CXCL8 modulates GluR1 phosphorylation.
The chemokine interleukin 8/CXCL8 induces the phosphorylation of the GluR1 subunit of the AMPA-type glutamate receptor in neurons and transfected HEK cells, on both serine 845 (S845) and 831 (S831) residues. We previously described that CXCL8 receptor CXCR2 and GluR1 co-precipitate and that GluR1/CXCR2 co-expression both in HEK cells and neurons impairs CXCL8-induced cell migration. Here we show that replacement of S845 with Ala (A), but not with Glu (E), strongly reduces GluR1/CXCR2 interaction and abolishes the impairment of CXCL8-induced cell migration. Considered together our findings point to the phosphorylated state of S845GluR1 as a determinant of GluR1-CXCR2 physical coupling.
Istituto Pasteur-Fondazione Cenci Bolognetti, Centro di Eccellenza BEMM, Università di Roma "Sapienza", P.le A. Moro 5, I00185 Roma, Italy.
Myriam
M
Catalano
Flavia
F
Trettel
Raffaela
R
Cipriani
Clotilde
C
Lauro
Fabrizia
F
Sobrero
Fabrizio
F
Eusebi
Cristina
C
Limatola
D000818:Animals; D000831:Animals, Newborn; D002118:Calcium / Q000378:metabolism; D002227:Carbazoles / Q000494:pharmacology; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology; D002614:Chelating Agents / Q000494:pharmacology; D002633:Chemotaxis / Q000187:drug effects; D000242:Cyclic AMP / Q000378:metabolism; D004533:Egtazic Acid / Q000031:analogs & derivatives / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects / Q000235:genetics; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D016209:Interleukin-8 / Q000494:pharmacology / Q000502:physiology*; D009154:Mutation; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D010766:Phosphorylation / Q000187:drug effects; D011758:Pyrroles / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors; D014162:Transfection
D002118:Calcium; D002227:Carbazoles; D002614:Chelating Agents; D000242:Cyclic AMP; D004533:Egtazic Acid; D004791:Enzyme Inhibitors; D016209:Interleukin-8; D011758:Pyrroles; D018091:Receptors, AMPA; C070379:1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester; C057416:KT 5720