[The spectrum studies of mechano-optical signal transduction with participation of LDH].
The optical response from a mechanical stimulus signal oscillating system of NADH, DPIP and O2 with the participant of lactate dehyrogenase (LDH) was studied with UV/Vis Spectrometer in the present paper. The signal transduction efficiency of the system was largely improved by the catalysis of LDH, when DPIP. : NADH was 1 : 4.5 (mole ratio)without lactate, and the average period of system was shortened from 108 to 34 min, and to 29 min with lactate existing. It was presumed that the accelerating effect of LDH was mainly brought by the activation of NADH at its catalysis center, and in addition, brought by the supplement of NADH through the dehydrogenation of lactate. The results indicated that the catalysis of enzyme would be put up also when only one of the two substrates existed.
Jiang
Ben-Guo
BG
Dalian Nationalities University, Key Lab of Biotechnology and Bioresources Utilization, SEAC-ME, Dalian 116600, China. jbg@dlnu.edu.cn
Zhang
Le
L
Fan
Sheng-Di
SD
D055162:Biocatalysis; D007770:L-Lactate Dehydrogenase / Q000032:analysis* / Q000378:metabolism; D009243:NAD / Q000378:metabolism; D015398:Signal Transduction; D013053:Spectrophotometry / Q000379:methods*; D013379:Substrate Specificity
D009243:NAD; D007770:L-Lactate Dehydrogenase