Intrinsic bent DNA colocalizes with the sequence involved in the Nd-sD mutation in the Bombyx mori fibroin light chain gene.
Multiple sequence alignments of the Bombyx mori fibroin light chain gene (fib-L) from hybrids and from Chinese and Japanese strains demonstrated that 51.6% of the fib-L third intron is conserved. One of these conserved segments, 41 bp long, contains the sequence CGTTATTATACATATT, which is duplicated in the B. mori Nd-s(D) mutant. In the present work, electrophoretic mobility assays and computational analyses revealed a major peak of intrinsic bent DNA within the segment that undergoes breakage in the previously-described Nd-s(D) mutation. This result suggested that this intrinsically-curved region might mediate DNA cleavage and enhance recombination events in the third intron of the Bombyx mori fib-L gene.
Departamento de Biologia Celular e Genetica, Universidade Estadual de Maringa, Maringa, Parana, Brazil. aparecidafernandez@gmail.com
Joice Felipes
JF
Barbosa
Juliana Pereira
JP
Bravo
Karen Izumi
KI
Takeda
Daniela Bertolini
DB
Zanatta
Jose Luis da Conceicao
JL
Silva
Valerio Americo
VA
Balani
Adriana
A
Fiorini
Maria Aparecida
MA
Fernandez
D000818:Animals; D001483:Base Sequence; D012831:Bombyx / Q000235:genetics* / Q000378:metabolism; D004247:DNA / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D024202:Electrophoretic Mobility Shift Assay; D005349:Fibroins / Q000235:genetics*; D007438:Introns / Q000235:genetics; D008969:Molecular Sequence Data; D009154:Mutation; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004247:DNA; D005349:Fibroins