Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine.
A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4'-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak AD-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 microg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV) and was within 12% at the limit of quantitation (0.5 microg/ml). Bias of the assay was <15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine.
Vega-Villa
Karina R
KR
College of Pharmacy, Department of Pharmaceutical Sciences and Pharmacology Toxicology Graduate Program, Washington State University, Pullman, Washington 99164-6534, USA.
Remsberg
Connie M
CM
Podelnyk
Kristy L
KL
Davies
Neal M
NM
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004355:Drug Stability; D005419:Flavonoids / Q000493:pharmacokinetics / Q000652:urine*; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D035501:Uncertainty
D005419:Flavonoids; C538973:isosakuranetin