Influence of amino nitrogen in the culture medium enhances the production of delta-endotoxin and biomass of Bacillus thuringiensis var. israelensis for the large-scale production of the mosquito control agent.
We reported here the role of amino nitrogen in the commercial production of Bacillus thuringiensis var. israelensis media design. The insect pathogen B. thuringiensis var. israelensis was cultured in different media containing varying initial levels of amino nitrogen sources obtained from three different commercial venders. The biomass, mosquito larval toxicity and spore count produced were measured during the fermentation process. The results showed that the higher level of initial amino nitrogen concentrations in the medium led to higher yield of biomass (dry weight 4.78 g l(-1)), larvicidal activity (LC(50) 18.52 ng ml(-1)) and spore count (3.24 x 10(11) CFU ml(-1)). Similarly decreasing the initial amino nitrogen concentration in the medium led to a decreased biomass (dry weight 1.64 g l(-1)), larvicidal activity (LC(50) 27.01 ng ml(-1)) and spore count (3.7 x 10(10) CFUml(-1)).
Prabakaran
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Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, Puducherry, 605006, India. gprabha_99@yahoo.com
Hoti
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D000330:Aedes / Q000187:drug effects; D000818:Animals; D001413:Bacillus thuringiensis / Q000187:drug effects* / Q000254:growth & development; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000633:toxicity; D018533:Biomass; D003470:Culture Media; D004731:Endotoxins / Q000096:biosynthesis* / Q000633:toxicity; D006460:Hemolysin Proteins / Q000096:biosynthesis* / Q000633:toxicity; D007306:Insecticides; D007814:Larva / Q000187:drug effects; D007928:Lethal Dose 50; D009032:Mosquito Control; D009584:Nitrogen / Q000032:analysis / Q000494:pharmacology*; D013997:Time Factors
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D003470:Culture Media; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; C030956:insecticidal crystal protein, Bacillus Thuringiensis; D009584:Nitrogen