The prophylactic protective effect of sesamol against ferric-nitrilotriacetate-induced acute renal injury in mice.
The aim of this study was to examine the prophylactic protective effects of 3,4-methylenedioxyphenol (sesamol) on ferric-nitrilotriacetate (Fe-NTA)-induced acute renal damage in mice. We induced acute renal injury in mice by treating them with 4 mg/kg of Fe-NTA for 3h. We used blood biochemistry, creatinine clearance, and histological examinations to assess renal function. With a high-performance chemiluminescence analyzer, we also determined the hydroxyl radical and superoxide anion levels (free radicals) generated. Renal xanthine oxidase activities were also assessed. Sesamol inhibited Fe-NTA-induced acute renal injury, renal lipid peroxidation, the levels of renal hydroxyl radical and superoxide anion generated, and the activity of xanthine oxidase in mice. Therefore, we concluded that sesamol protected mice against Fe-NTA-induced oxidative-stress-associated acute renal injury by at least partially inhibiting the production of reactive oxygen species.
Hsu
Dur-Zong
DZ
Department of Environmental and Occupational Health, National Cheng Kung University Medical College, 138 Sheng-Li Road, Tainan, Taiwan.
Wan
Chang-Hsin
CH
Hsu
Hua-Fen
HF
Lin
Yung-Ming
YM
Liu
Ming-Yie
MY
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D052117:Benzodioxoles / Q000494:pharmacology*; D001806:Blood Urea Nitrogen; D002614:Chelating Agents; D003404:Creatinine / Q000097:blood; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds; D017665:Hydroxyl Radical / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D007674:Kidney Diseases / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives*; D016877:Oxidants / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D020011:Protective Agents; D011506:Proteins / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism
D000975:Antioxidants; D052117:Benzodioxoles; D002614:Chelating Agents; D005290:Ferric Compounds; D016877:Oxidants; D010636:Phenols; D020011:Protective Agents; D011506:Proteins; D013481:Superoxides; D017665:Hydroxyl Radical; C025583:sesamol; D003404:Creatinine; D014969:Xanthine Oxidase; D009571:Nitrilotriacetic Acid; C020326:ferric nitrilotriacetate