A new fertility restorer locus linked closely to the Rfo locus for cytoplasmic male sterility in radish.
In this study, we have investigated a new fertility restorer (Rf) locus for cytoplasmic male sterility (CMS) in radish. We have obtained a CMS-Rf system consisting of sterile line '9802A1', maintainer line '9802B1' and restorer line '9802H'. F(1) plants from cross between sterile line '9802A1' and restorer line '9802H' were all male fertile, self pollination of F(1) plants produced an F(2) segregating population consisting of 600 individuals. The segregating population was found to fit a segregation ratio 3:1 for male fertile and sterile types, indicating that male fertility is restored by a single dominant gene (termed Rfo2) in the CMS-Rf system. Based on the DNA sequence of Rfo/Rfk1 (AJ535623), just one full length gene in the sterile line '9802A1', in the restorer line '9802H' and in the male fertile line '2006H', was cloned, respectively. The three sequences correspond to the same gene with two alleles: Rfob in '9802H' and rfob in '9802A1' and '2006H'. These two alleles differ from Rfo/Rfk1 and rfk1 (AJ535624) alleles by two synonymous base substitutions, respectively. Based on the differences between the Rfob and rfob genes, one PCR-based marker was developed, and designated Marker 1, which is identical to the corresponding region of Rfob by sequence analysis. In the F(2) segregating population described above, the Marker 1 was present in 5 sterile plants and in 453 fertile plants, absent in 4 fertile plants and in 138 sterile plants, and was found to fit a segregation ratio 3:1 indicating that Rfob was single copy in '9802H'. Linkage analysis showed that the Rfo2 locus for our CMS-Rf system was distant from the Rfo locus by about 1.6 cM. The sterile line '9802A1' was pollinated by the male fertile line '2006H' and the resulting F(1) plants were all male fertile. These results indicated that the male fertility of radish CMS can be restored by a new Rf locus, which linked tightly to the Rfo locus.
Wuhan Botanical Garden/Wuhan Institute of Botany, The Chinese Academy of Sciences, Wuhan, People's Republic of China.
Zhi Wei
ZW
Wang
Yu Jie
YJ
Zhang
Chang Ping
CP
Xiang
Shi Yong
SY
Mei
Yuan
Y
Zhou
Guo Pei
GP
Chen
Ting
T
Wang
D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D001483:Base Sequence; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000235:genetics* / Q000502:physiology; D018744:DNA, Plant / Q000235:genetics / Q000378:metabolism; D005298:Fertility / Q000235:genetics; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D005819:Genetic Markers / Q000235:genetics; D008969:Molecular Sequence Data; D051479:Plant Infertility / Q000235:genetics*; D011058:Pollen / Q000235:genetics; D016133:Polymerase Chain Reaction; D031224:Raphanus / Q000235:genetics*; D016415:Sequence Alignment; D014158:Transcription, Genetic
D018744:DNA, Plant; D005819:Genetic Markers