Hypoxia induces microRNA miR-210 in vitro and in vivo ephrin-A3 and neuronal pentraxin 1 are potentially regulated by miR-210.
Shortage of oxygen is one of the prime stress conditions in tissues. In this study, we looked for microRNAs expressed during hypoxia and showed that miR-210 expression was upregulated in response to hypoxia in vitro and in vivo. An active form of the HIF-1alpha induced the expression of miR-210, showing the involvement of the HIF-1 signaling pathway in miR-210 gene transcription. Furthermore, miR-210 was shown to bind to the predicted target sites of ephrin-A3 or neuronal pentraxin 1, causing repression in luciferase reporter activity. Contrary to the microRNA-mediated repression hypothesis, ephrin-A3 was expressed at very high levels in post-ischemic mouse hippocampus in vivo. Thus, the regulatory effects of miR-210 on its targets in vivo need to be further characterized.
Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Sciences, Kuopio University, P.O. Box 1627, FIN-70211 Kuopio, Finland.
Kati
K
Pulkkinen
Tarja
T
Malm
Mikko
M
Turunen
Jari
J
Koistinaho
Seppo
S
Ylä-Herttuala
D020413:3' Untranslated Regions / Q000737:chemistry; D000818:Animals; D002545:Brain Ischemia / Q000378:metabolism; D002097:C-Reactive Protein / Q000235:genetics* / Q000378:metabolism; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D036384:Ephrin-A3 / Q000235:genetics* / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011817:Rabbits; D000099153:Pentraxins
D020413:3' Untranslated Regions; D002097:C-Reactive Protein; D036384:Ephrin-A3; D035683:MicroRNAs; D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000099153:Pentraxins