Engineering of functional cartilage tissue using stem cells from synovial lining: a preliminary study.
Stem cells derived from synovial lining-synovial lining--derived stem cells or SDSCs--are a promising cell source for cartilage tissue engineering. We hypothesized that negatively selected SDSCs would form cartilage constructs and conventionally passaged SDSCs would be contaminated with macrophages, inhibiting SDSC-based chondrogenesis. We mixed SDSCs with fibrin gel and seeded the cells into polyglycolic acid scaffolds. After 3 days of incubation with a proliferative growth factor cocktail (containing transforming growth factor beta1 [TGF-beta1], insulin-like growth factor I [IGF-I], and basic fibroblast growth factor [FGF-2]), the cell-fibrin-polyglycolic acid constructs were transferred into rotating bioreactor systems and cultured with a chondrogenic growth factor cocktail (TGF-beta1/IGF-I) for up to 4 weeks. Tissue constructs based on negatively selected SDSCs had cartilaginous characteristics; were rich in glycosaminoglycans and collagen II; exhibited high expression of mRNA and protein for collagen II, aggrecan, and Sox 9; exhibited a negligible level of mRNA and protein for collagens I and X; and had an equilibrium modulus in the range of values measured for native human cartilage. Conventional passage yielded SDSCs with contaminating macrophages, which adversely affected the quality of tissue-engineered cartilage. We thus propose functional cartilage constructs could be engineered in vitro through the use of negatively isolated SDSCs.
Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, 3943 Health Sciences Center South, PO Box 9196, Morgantown, WV 26506-9196, USA. mpei@hsc.wvu.edu
Ming
M
Pei
Fan
F
He
Vincent L
VL
Kish
Gordana
G
Vunjak-Novakovic
D000818:Animals; D020219:Chondrogenesis / Q000187:drug effects; D006801:Humans; D007334:Insulin-Like Growth Factor I; D008264:Macrophages; D011100:Polyglycolic Acid / Q000737:chemistry; D033581:Stem Cell Transplantation; D013552:Swine; D013583:Synovial Membrane / Q000166:cytology*; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D016212:Transforming Growth Factor beta / Q000494:pharmacology
D016212:Transforming Growth Factor beta; D011100:Polyglycolic Acid; D007334:Insulin-Like Growth Factor I