Application of multivariate methods to the simultaneous Fourier Transform Infrared (FT-IR) spectrometric determination of stereo-isomers; quinine and quinidine.
The two stereo-isomers; quinine and quinidine have been determined in their mixtures in the IR region using chemometric multivariate methods, principal component regression (PCR) and partial least squares (PLS). A training set of thirty synthetic binary mixture solutions in the possible combinations containing 0.0 - 4.0 and 4.0 - 0.0% w/v quinine and quinidine, respectively in chloroform was used to develop the multivariate calibrations. A validation set containing thirty synthetic binary mixtures of variable ratios in the range of 0.2 - 4.0 and 4.0 - 0.2% w/v for quinine and quinidine, respectively in chloroform was used to validate the developed calibrations. The results of analysis of the validation synthetic mixtures were found to be 100.5+/-0.44% (R.S.D.%=0.44) and 100.5+/-0.38% (R.S.D.%=0.38) for quinine and 100.1+/-0.67% (R.S.D.%=0.67) and 100.1+/-0.68% (R.S.D.%=0.68) for quinidine using PCR and PLS models, respectively.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Alexandria, Egypt. abdel_azizwahbi@yahoo.com
Abdel-Aziz Mohamed
AA
Wahbi
Marwa Saio
MS
Moneeb
Ismail Ibrahim
II
Hewala
Mahmoud Farouk
MF
Bahnasy
D000962:Antimalarials / Q000032:analysis*; D001185:Artificial Intelligence; D002138:Calibration; D002626:Chemistry, Pharmaceutical; D007202:Indicators and Reagents; D016018:Least-Squares Analysis; D015999:Multivariate Analysis; D025341:Principal Component Analysis; D011802:Quinidine / Q000032:analysis*; D011803:Quinine / Q000032:analysis*; D012984:Software; D012996:Solutions / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013237:Stereoisomerism
D000962:Antimalarials; D007202:Indicators and Reagents; D012996:Solutions; D011803:Quinine; D011802:Quinidine