Role of chicken TL1A on inflammatory responses and partial characterization of its receptor.
The role of chicken TNF-like ligand 1A (ChTL1A) on inflammation and its receptor candidates was investigated to further understand its function as a proinflammatory cytokine. ChTL1A decreased the viability of CHO-K1 cells transfected with chicken TNFR2 or decoy receptor 3 and bound to TNFR2 and decoy receptor 3. ChTL1A was detected in chicken blood samples taken 4 h after LPS injection. Increased mRNA for inflammatory response-related factors such as IL-1beta, IL-6, ChTL1A, IFN-gamma, inducible NO synthase, and cyclooxygenase 2 were found in spleen samples following LPS injection. Ceruloplasmin and alpha(1) acid glycoprotein (as positive acute phase proteins) were increased in chicken plasma 12 h after ChTL1A injection. The injection of anti-ChTL1A Ab was able to prevent typical increases in plasma nitrite plus nitrate, ceruloplasmin, and alpha(1) acid glycoprotein concentrations following LPS injection. These results indicate that ChTL1A is a proinflammatory cytokine in chickens, animals that do not have TNF-alpha and lymphotoxin alpha orthologous genes, and that its proinflammatory action is, at least in part, expressed through binding to TNFR2.
Takimoto
Tetsuya
T
Laboratory of Animal Nutrition, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Sato
Kan
K
Akiba
Yukio
Y
Takahashi
Kazuaki
K
D000818:Animals; D030161:Avian Proteins / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D016466:CHO Cells; D002570:Ceruloplasmin / Q000037:antagonists & inhibitors / Q000378:metabolism; D002645:Chickens / Q000235:genetics / Q000276:immunology*; D006224:Cricetinae; D003412:Cricetulus; D015536:Down-Regulation / Q000235:genetics / Q000276:immunology; D007106:Immune Sera / Q000008:administration & dosage; D018836:Inflammation Mediators / Q000097:blood / Q000276:immunology / Q000378:metabolism / Q000502:physiology*; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000037:antagonists & inhibitors; D008297:Male; D011485:Protein Binding / Q000235:genetics / Q000276:immunology; D018124:Receptors, Tumor Necrosis Factor; D047889:Receptors, Tumor Necrosis Factor, Type II / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry / Q000276:immunology; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15 / Q000097:blood / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D014409:Tumor Necrosis Factor-alpha / Q000737:chemistry / Q000502:physiology
D030161:Avian Proteins; D007106:Immune Sera; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D018124:Receptors, Tumor Necrosis Factor; D047889:Receptors, Tumor Necrosis Factor, Type II; D011994:Recombinant Proteins; C117018:TL1 cytokine; D053380:Tumor Necrosis Factor Ligand Superfamily Member 15; D014409:Tumor Necrosis Factor-alpha; D002570:Ceruloplasmin