A bicistronic baculovirus vector for transient and stable protein expression in mammalian cells.
Baculoviruses are widely used for protein production in insect cells, and their potential for gene transfer to mammalian cells is increasingly being recognized. Here we describe a baculovirus vector with a bicistronic mammalian expression cassette and demonstrate its suitability for efficient transient and stable protein expression in human glioblastoma cells. Bicistronic baculovirus vectors are safe, cost efficient, and easy to produce; thus, they represent an excellent gene transfer system for mammalian cells.
Lackner
Andreas
A
Department of Medicine I, Institute of Cancer Research, Medical University of Vienna, A-1090 Vienna, Austria.
Genta
Kathrin
K
Koppensteiner
Herwig
H
Herbacek
Irene
I
Holzmann
Klaus
K
Spiegl-Kreinecker
Sabine
S
Berger
Walter
W
Grusch
Michael
M
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000235:genetics*; D005909:Glioblastoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D011506:Proteins / Q000235:genetics*; D013997:Time Factors; D014161:Transduction, Genetic
D011506:Proteins