Two-generation toxicity study on the copepod model species Tigriopus japonicus.
Previous studies on the intertidal copepod Tigriopus japonicus have demonstrated that it is a suitable model species for the assessment of acute toxicities of marine pollutants. In order to standardize T. japonicus for use in environmental risk assessment involving whole life cycle exposure, we tested nine pollutants for their effects on growth and reproduction during a two-generation life cycle exposure test. Nauplii (F 0) were exposed to a range of concentrations of each chemical in a static renewal culture system. Broods of the second generation (F1) were subsequently exposed to the same concentrations for one full life cycle. Of the seven traits (nauplius phase, development time, survival, sex ratio, number of clutch, nauplii per clutch and fecundity), only the length of the nauplius phase and development time showed a greater sensitivity to chemical exposure. Between the two sensitive traits, the period of the nauplius phase was more sensitive than cohort generation time. Biocides significantly increased the maturation period of nauplii as well as copepodids in F 0 generation. In this study, it was demonstrated that T. japonicus could also be used in reproduction and life cycle tests and it provides an opportunity for testing the chronic and subchronic toxic effects of marine pollutants. Further validation and harmonization in a multi-centric study involving other laboratories of the region will strengthen its use as a supplement to existing model species.
Lee
Kyun-Woo
KW
Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul, South Korea.
Raisuddin
Sheikh
S
Hwang
Dae-Sik
DS
Park
Heum Gi
HG
Dahms
Hans-Uwe
HU
Ahn
In-Young
IY
Lee
Jae-Seong
JS
D000818:Animals; D001152:Arsenicals; D033342:Copepoda / Q000502:physiology*; D003627:Data Interpretation, Statistical; D004202:Disinfectants / Q000633:toxicity; D052244:Endocrine Disruptors / Q000633:toxicity; D005260:Female; D007928:Lethal Dose 50; D008297:Male; D012098:Reproduction / Q000187:drug effects; D016019:Survival Analysis; D018675:Toxicity Tests; D014131:Trace Elements / Q000633:toxicity; D014873:Water Pollutants / Q000633:toxicity*
D001152:Arsenicals; D004202:Disinfectants; D052244:Endocrine Disruptors; D014131:Trace Elements; D014873:Water Pollutants