Crucial role of peroxiredoxin III in placental antioxidant defense of mice.
We observed frequent stillbirth in peroxiredoxin III (PrxIII) knockout maternal mice. Quantitative real time PCR (qRT-PCR) and Western-blot analysis revealed increased oxidative stress in placentas that were deficient in PrxIII. We did not find significant difference between PrxIII knockout maternal mice and wild-type littermates in hematological parameters, fetal number, and embryonic development. Nevertheless, we noticed enhanced expression of PrxI in erythrocytes of pregnant knockout mice. Our results provided in vivo evidence that PrxIII played a crucial role in placental antioxidant defense. Up-regulation of PrxI might provide a compensation that protected erythrocytes against oxidative damage.
Department of Cell Biology, Institute of Development, Aging, and Cancer, Tohoku University, Sendai 980-8575, Japan. lilq2005@126.com
Lianqin
L
Li
Wataru
W
Shoji
Hiroaki
H
Oshima
Masuo
M
Obinata
Manabu
M
Fukumoto
Naoko
N
Kanno
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D004906:Erythrocyte Count; D004912:Erythrocytes / Q000201:enzymology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018384:Oxidative Stress; D054464:Peroxiredoxins / Q000235:genetics / Q000502:physiology*; D010920:Placenta / Q000033:anatomy & histology / Q000201:enzymology* / Q000378:metabolism; D011247:Pregnancy; D050497:Stillbirth
D000975:Antioxidants; D054464:Peroxiredoxins