Morphine withdrawal increases metabotropic glutamate 2/3 receptors expression in nucleus accumbens.
We examined the effects of chronic morphine treatment and withdrawal on the expression of metabotropic glutamate (mGlu)1, mGlu5, and mGlu2/3 receptors in the nucleus accumbens and caudate putamen. Rats received a 14-day morphine treatment (escalating doses from 10 to 140 mg/kg). Receptor density was evaluated after chronic treatment and after 1, 3, and 14 days of withdrawal. mGlu1 and mGlu5 expression in the nucleus accumbens and in the caudate putamen was not affected by any of the experimental manipulations. In contrast, mGlu2/3 receptors in the nucleus accumbens, but not in the caudate putamen, increased at day 1, 3, and 14 of withdrawal. We suggest that an increased expression of mGlu2/3 receptors in the nucleus accumbens might contribute to the symptoms of morphine withdrawal.
Department of Human Physiology and Pharmacology, University of Rome La Sapienza, Rome, Italy.
Antonella M E
AM
Modafferi
Marco
M
Diana
Ferdinando
F
Nicoletti
Sergio
S
Scaccianoce
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects*; D008297:Male; D009020:Morphine / Q000009:adverse effects*; D009714:Nucleus Accumbens / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D018094:Receptors, Metabotropic Glutamate / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D013375:Substance Withdrawal Syndrome / Q000473:pathology*; D013997:Time Factors
D018094:Receptors, Metabotropic Glutamate; D009020:Morphine