Genome structure of the legume, Lotus japonicus.
The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L. japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10,951 complete and 19,848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L. japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes.
Sato
Shusei
S
Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba, Japan.
Nakamura
Yasukazu
Y
Kaneko
Takakazu
T
Asamizu
Erika
E
Kato
Tomohiko
T
Nakao
Mitsuteru
M
Sasamoto
Shigemi
S
Watanabe
Akiko
A
Ono
Akiko
A
Kawashima
Kumiko
K
Fujishiro
Tsunakazu
T
Katoh
Midori
M
Kohara
Mitsuyo
M
Kishida
Yoshie
Y
Minami
Chiharu
C
Nakayama
Shinobu
S
Nakazaki
Naomi
N
Shimizu
Yoshimi
Y
Shinpo
Sayaka
S
Takahashi
Chika
C
Wada
Tsuyuko
T
Yamada
Manabu
M
Ohmido
Nobuko
N
Hayashi
Makoto
M
Fukui
Kiichi
K
Baba
Tomoya
T
Nakamichi
Tomoko
T
Mori
Hirotada
H
Tabata
Satoshi
S
D002874:Chromosome Mapping; D018744:DNA, Plant; D020440:Gene Duplication; D017343:Genes, Plant; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D000070116:Lotus / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D026801:Synteny
D018744:DNA, Plant; D010940:Plant Proteins