Label-free sugar detection using phenylboronic acid-functionalized piezoresistive microcantilevers.
By appropriate surface functionalization using a thiolated phenylboronic acid derivative, gold-coated piezoresistive microcantilevers responsive to analytes having vicinal cis-diols were fashioned. A reference, uncoated cantilever permitted reliable subtraction of background signal (i.e., differential measurement). Experiments carried out in static mode under flow conditions, using the monosaccharide D-(-)-fructose as a test analyte in aqueous buffer, showed that the measured stationary-state surface stress was linear in analyte concentration over the 0-25 mM range, allowing this sugar to be readily detected at physiologically meaningful concentrations and at neutral pH. This approach offers a simple, reagentless, and flexible format for polyol screening that does not require aqueous alkaline solutions, suggesting potential applications in biomass process monitoring and glucose assay.
Baker
Gary A
GA
Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831, USA.
Desikan
Ramya
R
Thundat
Thomas
T
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D001897:Boronic Acids / Q000737:chemistry*; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D005632:Fructose / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D011955:Receptors, Drug / Q000737:chemistry*; D017655:Silicon Compounds / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013499:Surface Properties
C510906:4-mercaptophenylboronic acid; D001897:Boronic Acids; D011955:Receptors, Drug; D017655:Silicon Compounds; D013438:Sulfhydryl Compounds; D005632:Fructose; C032734:silicon nitride