Two-layered multiphoton microscopic imaging of cervical tissue.
We demonstrate the application of multiphoton microscopy (MPM) based on two-photon excited fluorescence and second harmonic generation signals in imaging two-layered structures of cervical tissue in mice. It was found that MPM is effective for visualizing two-layered cervical microstructures, monitoring the metabolic activity and the density (nuclear-to-cytoplasmic) of epithelial cells, measuring the size of epithelial cell nuclei and the thickness of the epithelial layer, and quantifying collagen in the stroma. These results suggest that, with the advent of clinical portability of typical multiphoton endoscopes, the MPM technique has the potential to be applied in vivo to the clinical diagnosis and monitoring of cervical cancer.
Zhuo
Shuangmu
S
Key Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Normal University, Ministry of Education, Fuzhou, 350007, China.
Chen
Jianxin
J
Luo
Tianshu
T
Jiang
Xingshan
X
Xie
Shusen
S
Chen
Rong
R
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D002584:Cervix Uteri / Q000033:anatomy & histology* / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism / Q000648:ultrastructure; D005260:Female; D005420:Flavoproteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D009243:NAD / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000175:diagnosis / Q000378:metabolism
D005420:Flavoproteins; D009243:NAD; D003094:Collagen