[The influence of ultraviolet radiation on HLA-DR+ and CD1a+ cells' number in systemic lupus erythematosus patients].
Systemic lupus erythematosus (SLE) is a chronic, autoimmune disease with complex etiology in which genetic, hormonal and environmental factors including ultraviolet radiation play an important role. Immune and inflammatory processes participate in skin lesions development and internal organ damage. Till now most of investigations estimated the role of inflammatory and immune response in skin lesions, but only scarce data evaluate these parameters in normal appearing skin in SLE patients. The aim of our study was to determine the influence of high dose UVB on the number of HLA-DR+ and CD1a+ cells in normal appearing skin of SLE patients. The study included 20 SLE patients and 14 healthy controls. The number of HLA-DR+ and CD1a+ cells were estimated by immunohistochemical method in two skin biopsies taken from non-irradiated and from irradiated sites, 24 h after exposure to 3 MED. All skin sections were stained with antibodies directed against HLA-DR antigen and CD1a molecule. The number of HLA-DR+ was statistically significantly higher in SLE patients comparing to healthy individuals, and UVB irradiation enhanced the difference. Similarly, the mean number of CD1a+ epidermal cells was significantly higher than in controls. In healthy subjects ultraviolet radiation caused migration of these cells into dermis, thus their number was lower in the epidermis. In contrast, in SLE patients under UVB CD1a+ cell count was significantly higher. The increased number of HLA-DR+ and epidermal CD1a+ cells in SLE may suggest that enhanced antigen presentation, leading to excessive cellular immune response and inflammatory reaction development, may result in induction or photoinduction of LE specific skin lesion.
Lesiak
Aleksandra
A
Katedra i Klinika Dermatologii i Wenerologii Uniwersytetu Medycznego w Lodzi.
Narbutt
Joanna
J
Kobos
Józef
J
Sysa-Jedrzejowska
Anna
A
Woźniacka
Anna
A
D000328:Adult; D018949:Antigens, CD1 / Q000032:analysis*; D001707:Biopsy, Needle; D016022:Case-Control Studies; D004817:Epidermis / Q000473:pathology* / Q000528:radiation effects; D005260:Female; D006684:HLA-DR Antigens / Q000032:analysis*; D006801:Humans; D008180:Lupus Erythematosus, Systemic; D008297:Male; D014466:Ultraviolet Rays / Q000009:adverse effects
D018949:Antigens, CD1; C419393:CD1a antigen; D006684:HLA-DR Antigens