Antimutagenic, antigenotoxic and antioxidant activities of Acacia salicina extracts (ASE) and modulation of cell gene expression by H2O2 and ASE treatment.
The total oligomers flavonoids (TOF), chloroform, petroleum ether and aqueous extracts from Acacia salicina, were investigated for the antioxidative, cytotoxic, antimutagenic and antigenotoxic activities. The viability of K562 cells were affected by all extracts after 48 h exposure. Our results showed that A. salicina extracts have antigenotoxic and/or antimutagenic activities. TOF and chloroform extracts exhibit antioxidant properties, expressed by the capacity of these extracts to inhibit xanthine oxidase activity. To further explore the mechanism of action of A. salicina extracts, we characterized expression profiles of genes involved in antioxidant protection and DNA repair in the human lymphoblastic cell line K562 exposed to H2O2. Transcription of several genes related to the thioredoxin antioxidant system and to the DNA base-excision repair pathway was up-regulated after incubation with chloroform, TOF and petroleum ether extracts. Moreover genes involved in the nucleotide-excision repair pathway and genes coding for catalase and Mn-superoxide-dismutase, two important antioxidant enzymes, were induced after incubation with the chloroform extract. Taken together, these observations provide evidence that the chloroform and TOF extracts of A. salicina leaves contain bioactive compounds that are able to protect cells against the consequences of an oxidative stress.
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Rue Avicenne 5000 Monastir, Tunisia.
Ines
I
Bouhlel
Kita
K
Valenti
Soumaya
S
Kilani
Ines
I
Skandrani
Mohamed
M
Ben Sghaier
Anne-Marie
AM
Mariotte
Marie-Genevieve
MG
Dijoux-Franca
Kamel
K
Ghedira
Isabelle
I
Hininger-Favier
François
F
Laporte
Leila
L
Chekir-Ghedira
D000045:Acacia / Q000737:chemistry*; D000818:Animals; D016587:Antimutagenic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D045744:Cell Line, Tumor; D020552:Comet Assay; D004247:DNA / Q000187:drug effects; D004338:Drug Combinations; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D005562:Formazans / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D005798:Genes, Bacterial / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D020014:K562 Cells / Q000187:drug effects / Q000378:metabolism; D008519:Medicine, Traditional; D020411:Oligonucleotide Array Sequence Analysis; D016877:Oxidants / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology; D051381:Rats; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins / Q000187:drug effects / Q000378:metabolism; D012486:Salmonella typhimurium / Q000187:drug effects / Q000235:genetics; D013778:Tetrazolium Salts / Q000378:metabolism
D016587:Antimutagenic Agents; D000975:Antioxidants; D004338:Drug Combinations; D005419:Flavonoids; D005562:Formazans; D016877:Oxidants; D010936:Plant Extracts; D000091243:Ribosomal Protein S9; D012269:Ribosomal Proteins; D013778:Tetrazolium Salts; C079782:MTT formazan; D004247:DNA; D006861:Hydrogen Peroxide