Synthesis, characterization, and DNA-binding of chiral complexes delta- and lambda-[Ru(bpy)2(pyip)]2+.
New chiral Ru(II) complexes delta and lambda-[Ru(bpy)(2)(pyip)](PF(6))(2) [(bpy = 2,2'-bipyridine; pyip = (2-(1-pyrenyl)-1H-imidazo[4,5-f] [1,10]phenanthroline] were synthesized and characterized by elemental analysis, (1)H NMR, ESI-MS, IR, and CD spectra. Their DNA-binding properties were studied by means of UV-vis, emission spectra, CD spectra and viscosity measurements. A subtle but detectable difference was observed in the interaction of both enantiomer with CT-DNA. Spectroscopy experiments indicated that each of these complexes could interact with the DNA. The DNA-binding of the Delta-enantiomer was stronger than that of Lambda-enantiomer. DNA-viscosity experiments provided evidence that both Delta- and Lambda-[Ru(bpy)(2)(pyip)](PF(6))(2) bound to DNA by intercalation. At the same time, the DNA-photocleavage properties of the complexes were investigated too. Under irradiation with UV light, Ru(II) complexes showed different efficiency of cleaving DNA.
Department of Chemistry, Tongji University, Shanghai 200092, China. shishuo@mail.tongji.edu.cn
Shuo
S
Shi
Tian-Ming
TM
Yao
Xiao-Ting
XT
Geng
Ling-Feng
LF
Jiang
Jie
J
Liu
Qing-Yuan
QY
Yang
Liang-Nian
LN
Ji
D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000378:metabolism*; D004278:DNA, Superhelical / Q000378:metabolism; D003851:Deoxyribonucleases / Q000378:metabolism; D004583:Electrons; D009942:Organometallic Compounds / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D010782:Photolysis; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D014783:Viscosity
D004278:DNA, Superhelical; D009942:Organometallic Compounds; C535160:bis(2,2'-bipyridine)bis(2-(1-pyrenyl)-1H-imidazo(4,5-f)(1,10)phenanthroline)ruthenium(II); D004247:DNA; C427203:calf thymus DNA; D003851:Deoxyribonucleases