Ogataea ganodermae sp. nov., a methanol-assimilating yeast species isolated from basidiocarps of Ganoderma sp.
Three methanol-utilizing yeast strains were isolated from basidiocarps of Ganoderma sp. collected from a tree trunk in Mangshan Mountain, Hunan Province, southern China. These strains formed hat-shaped ascospores in unconjugated and deliquescent asci. Sequence analysis of the large-subunit rRNA gene D1/D2 domain and internal transcribed spacer (ITS) region, electrophoretic karyotype comparison and phenotypic characterization demonstrated that the three strains represent a novel species of the genus Ogataea, which is described as Ogataea ganodermae sp. nov. (type strain SHS 2.1(T) =CGMCC AS 2.3435(T) =CBS 10646(T)). Phylogenetically, the novel species was closely related to Ogataea pini and Ogataea henricii. The latter two taxa with similar D1/D2 sequences were confirmed to represent separate species by ITS sequence and electrophoretic karyotype comparisons.
Ji
Zhao-Hui
ZH
Systematic Mycology and Lichenology Laboratory, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Bai
Feng-Yan
FY
D002681:China; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000032:analysis; D048690:Fruiting Bodies, Fungal; D032902:Ganoderma; D020459:Genes, rRNA; D007621:Karyotyping; D000432:Methanol / Q000378:metabolism*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D010641:Phenotype; D004718:Saccharomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013172:Spores, Fungal / Q000502:physiology
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D000432:Methanol