Comparison of the growth kinetics of neuropathogenic and nonneuropathogenic equid herpesvirus type 1 (EHV-1) strains in cultured murine neuronal cells and the relevance of the D/N(752) coding change in DNA polymerase gene (ORF30).
We compared the growth kinetics of neuropathogenic and nonneuropathogenic equine herpesvirus type 1 (EHV-1) strains in mouse cerebral cortex cells and investigated the relevance of the D/N amino acid change at position 752 of ORF30 in Japanese isolates. Neuropathogenic electropherotype P strains 01c1 and 89c25 exhibited similar growth kinetics to nonneuropathogenic P strain 90c16 in cultured neurons; however, the growth ability of type B strain 97c7 was lower than those of the other strains tested. The amino acid encoded at 752 of ORF30 in 01c1 was asparagic acid; asparagine was encoded in the other EHV-1 strains isolated from Japanese horses. The D/N(752) difference in ORF30 may not be related to replication ability in neurons.
Department of Applied Veterinary Sciences, United Graduate School of Veterinary Sciences, Gifu University, Gifu, Japan.
Souichi
S
Yamada
Tomio
T
Matsumura
Koji
K
Tsujimura
Tsuyoshi
T
Yamaguchi
Kenji
K
Ohya
Hideto
H
Fukushi
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry* / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D004861:Herpesvirus 1, Equid / Q000235:genetics* / Q000254:growth & development* / Q000472:pathogenicity; D007700:Kinetics; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D009474:Neurons / Q000166:cytology* / Q000821:virology*
D004259:DNA-Directed DNA Polymerase