Induction of the endoplasmic reticulum stress protein GADD153/CHOP by capsaicin in prostate PC-3 cells: a microarray study.
The effect of capsaicin, main pungent ingredient of hot chilli peppers, in the gene expression profile of human prostate PC-3 cancer cells has been analyzed using a microarray approach. We identified 10 genes that were down-regulated and five genes that were induced upon capsaicin treatment. The data obtained from microarray analysis were then validated using quantitative real-time PCR assays and Western blot analysis. The most remarkable change was the up-regulation of GADD153/CHOP, an endoplasmic reticulum stress-regulated gene. Activation of GADD153/CHOP protein was corroborated by immunofluorescence and Western blot. We then tested the contribution of GADD153/CHOP to protection against capsaicin-induced cell death using RNA interference. Blockage of GADD153/CHOP expression by small interfering RNA, significantly reduced capsaicin-induced cell death in PC-3 cells. Taken together, these results suggested that capsaicin induces the antiproliferative effect through a mechanism facilitated by ER stress in prostate PC-3 cells.
Sánchez
Ana María
AM
Department of Biochemistry and Molecular Biology, School of Medicine, University of Alcalá, 28871 Alcala de Henares, Madrid, Spain.
Martínez-Botas
Javier
J
Malagarie-Cazenave
Sophie
S
Olea
Nuria
N
Vara
Diana
D
Lasunción
Miguel Angel
MA
Díaz-Laviada
Inés
I
D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000235:genetics; D002211:Capsaicin / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004721:Endoplasmic Reticulum / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D011467:Prostate / Q000187:drug effects* / Q000378:metabolism; D034622:RNA Interference; D051742:Transcription Factor CHOP / Q000235:genetics* / Q000378:metabolism
D000970:Antineoplastic Agents; D051742:Transcription Factor CHOP; D002211:Capsaicin