Selective cyclooxygenase inhibitors prevent the growth of Chlamydia pneumoniae in HL cells.
The effects of the selective cyclooxygenase (COX) inhibitors SC-560 and PTPBS were studied in Chlamydia pneumoniae-infected HL cell cultures. Chlamydia pneumoniae growth and viability were assessed by quantifying inclusions and re-passages. COX-1 and COX-2 mRNA expression in HL cells during chlamydial infection was quantified with real-time polymerase chain reaction. SC-560 (10 microg/mL) and PTPBS (18 microg/mL) completely inhibited the growth of C. pneumoniae and the effect was dose-dependent between 4-9 microg/mL and 2-16 microg/mL, respectively. Inclusion size was reduced from 11.5+/-1.3 microm to 1.9+/-0.7 microm in the presence of the drugs. Removing the drugs returned the size to normal and increased the number of inclusions. Selective COX inhibitors appear to have a chlamydiostatic but not chlamydiacidic effect; they inhibit the growth of C. pneumoniae in vitro but do not prevent infection or eradicate C. pneumoniae from host cells.
Yan
Ying
Y
Department of Medical Microbiology, University of Oulu, Aapistie 5A, Oulu, Finland. ying.yan@oulu.fi
Silvennoinen-Kassinen
Sylvi
S
Törmäkangas
Liisa
L
Leinonen
Maija
M
Saikku
Pekka
P
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002460:Cell Line; D016993:Chlamydophila pneumoniae / Q000187:drug effects* / Q000254:growth & development; D051545:Cyclooxygenase 1 / Q000096:biosynthesis; D051546:Cyclooxygenase 2 / Q000096:biosynthesis; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D006801:Humans; D002479:Inclusion Bodies / Q000382:microbiology; D008214:Lymphocytes / Q000382:microbiology*; D050296:Microbial Viability; D011720:Pyrazoles
D000900:Anti-Bacterial Agents; D016861:Cyclooxygenase Inhibitors; D011720:Pyrazoles; C115461:SC 560; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496540:PTGS2 protein, human