Electrochemical approach to detect apoptosis.
This paper reports an electrochemical approach for detection of apoptosis. Here we prepare a gold electrode modified with a helix peptide ferrocene (Fc)-GDGDEVDGC. Fc is used as an electroactive reporter and the peptide as a recognition and cleavage site of caspase-3, which is a special proteinase to apoptosis. Results show that this method may sensitively and specifically detect apoptotic cells with signal decline of 85%. This approach is different from the previous methods for apoptosis detection, because it does not need any fluorescent materials, expensive biological instruments, or complicated procedures.
Xiao
Han
H
Liu
Lei
L
Meng
Fanben
F
Huang
Junyi
J
Li
Genxi
G
D017209:Apoptosis / Q000502:physiology*; D053148:Caspase 3 / Q000737:chemistry / Q000378:metabolism; D045744:Cell Line, Tumor; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods*; D004566:Electrodes; D005296:Ferrous Compounds / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D000075163:Metallocenes; D009842:Oligopeptides / Q000737:chemistry
D005296:Ferrous Compounds; D000075163:Metallocenes; D009842:Oligopeptides; D006046:Gold; D053148:Caspase 3; C004998:ferrocene