Imaging polyhedral inclusion bodies of nuclear polyhedrosis viruses with second harmonic generation microscopy.
We studied the polarization anisotropy of second harmonic generation (SHG) in polyhedral inclusion bodies (PIBs) of nuclear polyhedrosis viruses (NPV). Due to a body-centered-cubic arrangement of polyhedrin trimers, a characteristic SHG polarization property with a mixture of I23 and I3 symmetry was measured from PIBs. With this characteristic SHG anisotropy, it provides an intrinsic nonlinear signal for virus infection studies in living cells. With multimodal harmonic generation microscopy, we also demonstrated 3D imaging on PIBs of NPV in living cells. The distribution and the number of PIBs in intact infected cells can be revealed without the help of fluorescent labeling.
Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei 10617, Taiwan, ROC.
Tzu-Ming
TM
Liu
Yen-Wei
YW
Lee
Chieh-Feng
CF
Chang
Shih-Chia
SC
Yeh
Chung-Hsiung
CH
Wang
Shi-Wei
SW
Chu
Chi-Kuang
CK
Sun
D007089:Image Enhancement / Q000295:instrumentation* / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000379:methods*; D002479:Inclusion Bodies / Q000648:ultrastructure*; D008859:Microscopy, Polarization / Q000295:instrumentation* / Q000379:methods; D017924:Nucleopolyhedroviruses / Q000648:ultrastructure*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity