Cloning of an ovule specific promoter from Arabidopsis thaliana and expression of beta-glucuronidase.
Tissue specific expression of transgenes in plant species has several advantages over constitutive expression. Identification of ovule specific promoters would be useful in genetic engineering of plants with a variety of desirable traits such as genetically engineered parthenocarpy, female sterile plants or seedless fruits. Relative inaccessibility and difficulty in harvesting adequate amounts of tissue at known developmental stages has impeded the progress in cloning of promoters involved in ovule development. In the present study an ovule specific promoter was cloned from Arabidopsis AGL11 gene and used to express GUS (beta-glucuronidase) gene in transgenic Arabidopsis. Histochemical staining of GUS appeared in the center of young ovary (ovules), but no detectable GUS activity was observed in vegetative plant tissues, sepals, petals and androecium. AGL11 gene promoter can be useful to modify the developmental path of plants by expressing either plant hormones or lethal genes for agronomic purpose.
National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110 012, India.
Vikrant
V
Nain
Anju
A
Verma
Neeraj
N
Kumar
Priyanka
P
Sharma
B
B
Ramesh
P Ananda
PA
Kumar
D026342:AGAMOUS Protein, Arabidopsis / Q000235:genetics; D017360:Arabidopsis / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D001483:Base Sequence; D003001:Cloning, Molecular; D017124:Conserved Sequence; D035264:Flowers / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000235:genetics*; D018398:Homeodomain Proteins / Q000235:genetics; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000235:genetics; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics; D019076:Transgenes
D026342:AGAMOUS Protein, Arabidopsis; D018398:Homeodomain Proteins; D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors; C097416:floral-binding protein 11, Petunia; D005966:Glucuronidase