An ultraviolet melting study of the stability of the DNA double helix in the NaDNA-bipyridyl-(ethylenediamine)platintum(II) complex.
Complexes of NaDNA with the bipyridyl-(ethylenediamine)platintum(II) (abbreviated [(bipy)Pt(en)]2+) molecular ion have been studied in solution via ultraviolet absorption experiments at 260 nm between 50 and 90 degrees C. These measurements, performed as a function of the molar ratio of the [(bipy)Pt(en)]2+ complex to DNA base pairs, show that the stability of the DNA double helix is increased by the formation of the DNA.[(bipy)Pt(en)]2+ complex: at a molar ratio of 0.33, the temperature at which the DNA double helix separates into two single strands is increased by about 15 degrees C.
Szabó
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Department of Physics and Astronomy, University of Toledo, Toledo, OH 43606, USA.
Lee
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SA
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives* / Q000737:chemistry / Q000528:radiation effects; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D018736:DNA Adducts / Q000737:chemistry* / Q000528:radiation effects*; D009944:Organoplatinum Compounds / Q000737:chemistry* / Q000528:radiation effects*; D013816:Thermodynamics; D014466:Ultraviolet Rays
D018736:DNA Adducts; C460911:DNA-bipyridyl-(ethylenediamine)platinum(II); D009944:Organoplatinum Compounds; D015082:2,2'-Dipyridyl; D004247:DNA