Immunohistochemical detection of Ndrg2 in the mouse nervous system.
NDRG2, a member of the N-myc downstream-regulated gene (NDRG) family, is involved in cell differentiation and development. However, the distribution and function of Ndrg2 in the central nervous system remains unclear. Here, we analyzed the expression and distribution of Ndrg2 in the mouse brain and explored the potential physiological functions of Ndrg2. Ndrg2 was expressed in different regions of the brain, including the cerebral cortex, olfactory bulb, midbrain, hippocampus, and thalamus, with high levels in the midbrain and thalamus. Immunohistochemistry assay revealed that Ndrg2-positive cells distributed widely in the adult mouse brain and some of them showed nuclear staining. Indirect immunofluorescence and confocal microscopy studies showed that Ndrg2 protein colocalized with glial fibrillary acidic protein, indicating that Ndrg2 is expressed in astrocytes. Furthermore, Ndrg2 expression increased in glioma cells that were differentiating into astrocytes. Taken together, these findings suggest that Ndrg2 is possibly associated with glial cell proliferation and differentiation based on its immunolocalization in this study.
Shen
Lan
L
The Institute of Molecular Biology and The State Key Laboratory of Cancer Biology, The Fourth Military Medical University, Xi'an, China.
Zhao
Zhen-Yu
ZY
Wang
Ya-Zhou
YZ
Ji
Shao-Ping
SP
Liu
Xin-Ping
XP
Liu
Xue-Wu
XW
Che
Hong-Lei
HL
Lin
Wei
W
Li
Xia
X
Zhang
Jian
J
Yao
Li-Bo
LB
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D045744:Cell Line, Tumor; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005910:Glioma / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D015850:Interleukin-6 / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D009420:Nervous System / Q000033:anatomy & histology / Q000378:metabolism*; D009447:Neuroblastoma / Q000378:metabolism; D011506:Proteins / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
D048868:Adaptor Proteins, Signal Transducing; D005904:Glial Fibrillary Acidic Protein; D015850:Interleukin-6; C485172:Ndr2 protein, mouse; D011506:Proteins