Method for electroporation for the early chick embryo.
In vitro whole-embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures, and some experimental examples are presented.
Division of Morphogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan.
Jun
J
Hatakeyama
Kenji
K
Shimamura
D000818:Animals; D002642:Chick Embryo; D015509:Developmental Biology / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D046149:Embryo Culture Techniques / Q000379:methods*; D018014:Gene Transfer Techniques; D049452:Green Fluorescent Proteins / Q000737:chemistry; D010957:Plasmids / Q000378:metabolism; D013997:Time Factors; D014162:Transfection
D049452:Green Fluorescent Proteins