Dendritic cell uptake of iron-based magnetic nanoparticles.
We have investigated the internalization of magnetic nanoparticles (NPs) into dendritic cells (DCs) in order to assess both the final location of the particles and the viability of the cultured cells. The particles, consisting of a metallic iron core covered with carbon, showed no toxic effects on the DCs and had no effect in their viability. We found that mature DCs are able to incorporate magnetic nanoparticles in a range of size from 10 nm to ca. 200 nm, after 24 h of incubation. We describe a method to separate cells loaded with NPs, and analyze the resulting material by electron microscopy and magnetic measurements. It is found that NPs are internalized in lysosomes, providing a large magnetic signal. Our results suggest that loading DCs with properly functionalized magnetic NPs could be a promising strategy for improved vectorization in cancer diagnosis and treatment.
Goya
G F
GF
Aragon Institute of Nanoscience (INA), University of Zaragoza, and Hospital Universitario Lozano Blesa, Pedro Cerbuna 12, 50009 Zaragoza, Spain. goya@unizar.es
Marcos-Campos
I
I
Fernández-Pacheco
R
R
Sáez
B
B
Godino
J
J
Asín
L
L
Lambea
J
J
Tabuenca
P
P
Mayordomo
J I
JI
Larrad
L
L
Ibarra
M R
MR
Tres
A
A
D002469:Cell Separation / Q000379:methods*; D003713:Dendritic Cells / Q000378:metabolism* / Q000648:ultrastructure; D006801:Humans; D008247:Lysosomes / Q000378:metabolism; D008280:Magnetics; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission