Responses of biochemical markers in carp Cyprinus carpio from two field sites in Western Ukraine.
The aim of this study was to compare environmental quality in two sites of the river in Western Ukraine, rural (R) and industrial (I) during three seasons via a set of biochemical markers in carp Cyprinus carpio L. Upon comparing the values of the I-site with those of the R-site, we found that Mn- and Cu, Zn-superoxide dismutase (SOD) activities decreased and O(.) production increased; metallothionein (MT) and glutathione levels increased in most cases, and acetylcholinesterase (AChE) activity decreased in summer. This confirms our hypothesis about continuous environmental press at the I-site. The higher activity of catalase and lipid peroxidation (TBARS), as well as the increased levels of vitellogenin-like proteins at the R-site, compare to the I-site in spring reflects the permitting effect of agricultural discharges. According to the results of PCA, the most sensitive biomarkers of pollution are MT, TBARS, and AChE in liver.
Department of Chemistry, Ternopil National Pedagogical University, Kryvonosa Street 2, 46027 Ternopil, Ukraine.
Halina I
HI
Falfushynska
Oksana B
OB
Stolyar
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D002347:Carps / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D004784:Environmental Monitoring / Q000379:methods; D029941:Fish Proteins / Q000378:metabolism*; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008668:Metallothionein / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D025341:Principal Component Analysis; D045483:Rivers / Q000737:chemistry; D012621:Seasons; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances; D014455:Ukraine; D014819:Vitellogenins / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000032:analysis / Q000633:toxicity*
D015415:Biomarkers; D029941:Fish Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014819:Vitellogenins; D014874:Water Pollutants, Chemical; D008668:Metallothionein; D002374:Catalase; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; D005978:Glutathione