Developing cryopreservation for Picea sitchensis (sitka spruce) somatic embryos: a comparison of vitrification protocols.
Two vitrification-based cryopreservation protocols, encapsulation/dehydration and PVS2 were applied to Stage 2 (globular) and Stage 4 (torpedo) somatic embryos (SE) from Picea sitchensis. Two recovery responses: partially differentiated embryogenic suspensor masses (ESM) and dedifferentiated non-embryogenic masses (NEM) were observed following exposure to LN. All genotypes tested, proliferated NEM, approximately 10 to 100% of the total SE cryopreserved. A General Linear Model applied to NEM recovery data demonstrated several different factors (developmental state and genotype, treatment, culture age) interacted at a significant level (P less than 0.05) to influence proliferation. One genotype was capable of proliferating ESM after cryopreservation using encapsulation-dehydration, this response was achieved for Stage 4 embryos derived from the youngest ESM tissue.
Gale
Samantha
S
Plant Conservation Group, Univ of Abertay Dundee, UK.
John
Allan
A
Harding
Keith
K
Benson
Erica E
EE
D015925:Cryopreservation / Q000379:methods*; D005612:Freeze Drying; D005838:Genotype; D016014:Linear Models; D028222:Picea / Q000235:genetics / Q000502:physiology*; D012639:Seeds / Q000502:physiology*