Renal ischemia and reperfusion injury: influence of chorpromazine on renal function and lipid peroxidation.
OBJECTIVE
To evaluate the influence of chlorpromazine (CPZ) on renal function and lipid peroxidation in a rat model of kidney ischemia/reperfusion injury.


METHODS
Forty eight Wistar rats underwent a laparotomy for hilar clamping of left kidney with a bulldog clamp for 60 minutes followed by organ reperfusion and contralateral nephrectomy. Of these, 26 received 3mg/kg of CPZ intravenously 15 minutes before renal ischemia (G-E) while the remaining 22 were used as ischemic control group (G-C). Eleven rats of G-E and 8 of G-C were followed for blood urea nitrogen and creatinine determinations before renal ischemia and at 1st, 4th and 7th postoperative days. Samplings of left renal tissue were obtained at 5 minutes (5 rats from each group) and 24 hours (9 G-C and 10 of G-E) of reperfusion for malondialdehy (MDA) content determination. Controls of renal MDA content were determined in kidneys harvested from 6 additional normal rats.


RESULTS
Acute renal failure occurred in all animals but levels of BUN and creatinine were significantly lower in G-E (p<0.001). MDA content rose strikingly at 5 minutes of reperfusion in both groups (p>0.05) and returned near to normal levels 24 hours later.


CONCLUSIONS
CPZ conferred partial protection of renal function to kidneys submitted to ischemia/reperfusion injury that seems to be not dependent on inhibition of lipid peroxidation.
Tucci Junior
Silvio
S
Division of Urology, Department of Surgery and Anatomy, Ribeirão Preto Faculty of Medicine, University of São Paulo, SP, Brazil. stucci@convex.com.br
Carvalho
Roberto Marins de
RM
Celini
Fábia Martins
FM
Cologna
Adauto José
AJ
Suaid
Haylton Jorge
HJ
Tirapelli
Luis Fernando
LF
Martins
Antonio Carlos Pereira
AC
D000818:Animals; D015415:Biomarkers / Q000097:blood; D002746:Chlorpromazine / Q000494:pharmacology*; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D018492:Dopamine Antagonists / Q000494:pharmacology*; D007511:Ischemia / Q000150:complications*; D041781:Jaundice, Obstructive / Q000188:drug therapy; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects* / Q000503:physiopathology; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009392:Nephrectomy; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000503:physiopathology / Q000517:prevention & control*; D013997:Time Factors; D014508:Urea / Q000097:blood
D015415:Biomarkers; D018492:Dopamine Antagonists; D008315:Malondialdehyde; D014508:Urea; D003404:Creatinine; D002746:Chlorpromazine