Single muscle fiber contractile properties of young competitive distance runners.
The purpose of this investigation was to characterize the contractile properties of individual slow- and fast-twitch myofibers from highly trained distance runners. Muscle biopsies were obtained from the gastrocnemius of eight competitive runners (Run) and eight recreationally active individuals (Rec). Slow-twitch [myosin heavy chain (MHC) I] and fast-twitch (MHC IIa) myofibers were isolated and analyzed for diameter (microm), peak force (Po; mN), unloaded contraction velocity (Vo; fiber lengths/s), and power. Maximum oxygen uptake was higher (P<0.05) in Run (71+/-1 vs. 47+/-2 ml.kg(-1).min(-1)). Diameter of MHC I and MHC IIa fibers from Run subjects was approximately 20% greater (P<0.05) than Rec. Peak force of the MHC IIa fibers was 31% higher (P<0.05) in Run, whereas Po of MHC I fibers was not different between groups. No differences for specific tension (Po/cross-sectional area) were present between groups for either fiber type. Vo was higher (P<0.05) in MHC I (+70%) and MHC IIa (+18%) fibers from Run subjects. In vitro peak absolute power (microN.s(-1)) of both fiber types was greater (P<0.05) in Run (131 and 85% for MHC I and MHC IIa, respectively). Additionally, normalized power (W/l) of the MHC I fibers was 64% higher in Run, whereas no differences were noted for normalized power of MHC IIa fibers. These data indicate that highly trained endurance runners have elevated contraction velocity in both slow- and fast-twitch myofibers. These characteristics of the fast-twitch muscle fibers have not been previously reported in competitive endurance athletes and may contribute to the high level of running performance in these athletes.
Human Performance Laboratory, Ball State University, Muncie, IN 47306, USA. mharber@bsu.edu
Matthew
M
Harber
Scott
S
Trappe
D000328:Adult; D015308:Anaerobic Threshold / Q000502:physiology; D006801:Humans; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D018485:Muscle Fibers, Skeletal / Q000502:physiology* / Q000648:ultrastructure; D018482:Muscle, Skeletal / Q000166:cytology / Q000502:physiology* / Q000648:ultrastructure; D018995:Myosin Heavy Chains / Q000378:metabolism; D024461:Myosin Type I / Q000378:metabolism; D024462:Myosin Type II / Q000378:metabolism; D010807:Physical Endurance / Q000502:physiology*; D012420:Running / Q000502:physiology*
D024461:Myosin Type I; D024462:Myosin Type II; D018995:Myosin Heavy Chains