Artificial DNA made exclusively of nonnatural C-nucleosides with four types of nonnatural bases.
We describe a new class of DNA-like oligomers made exclusively of nonnatural, stable C-nucleosides. The nucleosides comprise four types of nonnatural bases attached to a deoxyribose through an acetylene bond with the beta-configuration. The artificial DNA forms right-handed duplexes and triplexes with the complementary artificial DNA. The hybridization occurs spontaneously and sequence-selectively, and the resulting duplexes have thermal stabilities very close to those of natural duplexes. The artificial DNA might be applied to a future extracellular genetic system with information storage and amplifiable abilities.
Graduate School of Pharmaceutical Sciences, University of Toyama, Sugitani 2630, Toyama 930-0194, Japan.
Yasuhiro
Y
Doi
Junya
J
Chiba
Tomoyuki
T
Morikawa
Masahiko
M
Inouye
D000114:Acetylene / Q000737:chemistry; D003562:Cytidine / Q000031:analogs & derivatives*; D003596:Cytosine / Q000031:analogs & derivatives*; D004247:DNA / Q000138:chemical synthesis / Q000737:chemistry*; D003855:Deoxyribose / Q000737:chemistry; D006358:Hot Temperature; D006860:Hydrogen Bonding; D009690:Nucleic Acid Conformation; D009943:Organophosphorus Compounds / Q000737:chemistry; D013816:Thermodynamics
D009943:Organophosphorus Compounds; C434331:phosphoramidite; D003855:Deoxyribose; D003562:Cytidine; D003596:Cytosine; D004247:DNA; D000114:Acetylene