[Intraspecies divergence of 16S rDNA, ITS, rpoB gene and hsp65 gene sequence for Mycobacterium lentiflavum].
OBJECTIVE
To clarify the genetic microheterogeneity of Mycobacterium lentiflavum and identify the predominant genotype.


METHODS
Clinical isolates of M. lentiflavum used in this study were obtained from sixteen patients of lung diseases. In order to assess their intraspecies variability, four gene fragments, from the 16S rDNA (1471 bp), 16S-23S ITS (282 bp), rpoB (306 bp), and hsp65 (401 bp), were sequenced.


RESULTS
Intraspecies variabilities were found in all of the four targeting fragments. As multilocus sequence typing with these four targets, 16 clinical isolates were divided into 3 genotypes, i.e., MLST2, MLST3, and MLST4. Among them, MLST2 to which 12 clinical isolates belonged, was a predominant genotype. Three strains belonged to MLST3 and the remaining one strain belonged to MLST4. Drug susceptibility study indicated that there was no clear relation between sequence types and drug susceptibility.


CONCLUSIONS
Multilocus sequence typing could aid in characterization and in better understanding of the epidemiology of M. lentiflavum.
Kobe Institute of Health, 4-6, Minatojima-nakamachi, Chuo-ku, Kobe-shi, Hyogo 650-0046, Japan. kx2t-iwmt@asahi-net.or.jp
Tomotada
T
Iwamoto
Kazue
K
Nakanaga
Norihisa
N
Ishii
Shiomi
S
Yoshida
Hajime
H
Saito
D001426:Bacterial Proteins / Q000235:genetics*; D001483:Base Sequence / Q000235:genetics; D018834:Chaperonin 60; D018833:Chaperonins / Q000235:genetics*; D012321:DNA-Directed RNA Polymerases; D018740:Genetic Heterogeneity; D005838:Genotype; D006801:Humans; D009161:Mycobacterium / Q000235:genetics*; D009165:Mycobacterium Infections, Nontuberculous / Q000382:microbiology; D012329:RNA, Bacterial / Q000235:genetics*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D014397:Tuberculosis, Pulmonary / Q000382:microbiology
D001426:Bacterial Proteins; D018834:Chaperonin 60; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; C074119:heat-shock protein 65, Mycobacterium; C505805:rpoB protein, Mycobacterium tuberculosis; D012321:DNA-Directed RNA Polymerases; D018833:Chaperonins