Vaccinia peptides eluted from HLA-DR1 isolated from virus-infected cells are recognized by CD4+ T cells from a vaccinated donor.
Class II MHC proteins bind peptides and present them to CD4 (+) T cells as part of the immune system's surveillance of bodily tissues for foreign and pathogenic material. Antigen processing and presentation pathways have been characterized in detail in normal cells, but there is little known about the actual viral peptides that are presented to CD4 (+) T cells that signal infection. In this study, two-dimensional LC-MS/MS was used to identify vaccinia virus-derived peptides among the hundreds to thousands of peptide antigens bound to the human class II MHC protein HLA-DR1 on the surface of vaccinia virus-infected cells. The peptides, derived from the I6L, D6R, and A10L viral proteins, were 15 residues in length, bound efficiently to HLA-DR1 as synthetic peptides, and were recognized by vaccinia-specific CD4 (+) T cells obtained from an immunized donor.
Department of Pathology, Proteomic and Mass Spectrometry Core Facility, Center for Infectious Disease and Vaccine Research, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.
Iwona
I
Strug
J Mauricio
JM
Calvo-Calle
Karin M
KM
Green
John
J
Cruz
Francis A
FA
Ennis
James E
JE
Evans
Lawrence J
LJ
Stern
D000595:Amino Acid Sequence; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D018984:Epitopes, T-Lymphocyte; D015798:HLA-DR1 Antigen / Q000276:immunology*; D006801:Humans; D008969:Molecular Sequence Data; D010455:Peptides / Q000276:immunology*; D012900:Smallpox Vaccine / Q000276:immunology; D053719:Tandem Mass Spectrometry; D014611:Vaccination; D014616:Vaccinia virus / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology*
D018984:Epitopes, T-Lymphocyte; D015798:HLA-DR1 Antigen; D010455:Peptides; D012900:Smallpox Vaccine; D014764:Viral Proteins