O-selective condensation using P-N bond cleavage in RNA synthesis without base protection.
In RNA synthesis without base protection, a new method for O-selective condensation with more than 99% selectivity was developed by 6-nitro-HOBt-mediated cleavage of undesired P(III)-N bonds on nucleobase moieties. Moreover, we for the first time succeeded in synthesizing oligoRNAs without base protection.
Ohkubo
Akihiro
A
Department of Life Science, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226-8501, Japan.
Kuwayama
Yasukazu
Y
Kudo
Tomomi
T
Tsunoda
Hirosuke
H
Seio
Kohji
K
Sekine
Mitsuo
M
D002851:Chromatography, High Pressure Liquid; D019281:Dimerization; D015394:Molecular Structure; D009584:Nitrogen / Q000737:chemistry*; D017905:Phosphites / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry*; D012313:RNA / Q000138:chemical synthesis* / Q000737:chemistry
D017905:Phosphites; D010758:Phosphorus; D012313:RNA; D009584:Nitrogen