[Study on correlation between Atractylodes macrocephala viability and germination rate].
OBJECTIVE
To screen the optimized methods for detection seed viability and germination rate determination of Atractylodes macrocephala, and determine the relationship between seed viability and germination rate.


METHODS
There were four methods, which including 2,3,5-triphynel tetrazolilum chloride (TTC) staining, red ink staining, BTB staining and Nongjia method, to evaluate the 12 A. macrocephala local varieties'seed viability and measure their germination rate.


RESULTS
Seed viability of A. macrocephala using TTC staining ranked the first compared to that of other three methods. Seed viability was significantly related with germination rate using TTC method. Their correlation coefficient reached 0.915 and regression equation was also found out between seed viability (X) and germination rate (Y), which was Y = -0.083 4 + 0.995 4X.


CONCLUSIONS
TTC staining was the optimal method to determine A. macrocephala seed vitality. Furthermore, seed viability was significant related with germination rate of A. macrocephala.
Zhejiang Research Institute of Traditional Chinese Medicine, Hangzhou 310023, China.
Yu-Feng
YF
Shen
Zhi-An
ZA
Wang
Xu-Ping
XP
Yu
Xiao-Xia
XX
Shen
D031174:Atractylodes / Q000502:physiology*; D018525:Germination / Q000502:physiology*; D010946:Plants, Medicinal / Q000502:physiology; D012639:Seeds / Q000502:physiology*