High-performance liquid chromatography analysis methods developed for quantifying enzymatic esterification of flavonoids in ionic liquids.
Methods using reversed-phase high-performance liquid chromatography (RP-HPLC) with ELSD were investigated to quantify enzymatic reactions of flavonoids with fatty acids in the presence of diverse room temperature ionic liquids (RTILs). A buffered salt (preferably triethylamine-acetate) was found essential for separation of flavonoids from strongly polar RTILs, whereby RTILs were generally visible as two major peaks identified based on an ion-pairing/exchanging hypothesis. C8 and C12 stationary phases were optimal while mobile phase pH (3-7) had only a minor influence on separation. The method developed was successfully applied for primary screening of RTILs (>20), with in depth evaluation of substrates in 10 RTILs, for their evaluation as reaction media.
National Food Institute, Technical University of Denmark, Søltofts Plads, Building 227, 2800 Kgs. Lyngby, Denmark.
Bena-Marie
BM
Lue
Zheng
Z
Guo
Xuebing
X
Xu
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004951:Esterification; D005419:Flavonoids / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D052578:Ionic Liquids / Q000737:chemistry*; D015394:Molecular Structure
D005419:Flavonoids; D052578:Ionic Liquids