Genome structure of the legume, Lotus japonicus.
The legume Lotus japonicus has been widely used as a model system to investigate the genetic background of legume-specific phenomena such as symbiotic nitrogen fixation. Here, we report structural features of the L. japonicus genome. The 315.1-Mb sequences determined in this and previous studies correspond to 67% of the genome (472 Mb), and are likely to cover 91.3% of the gene space. Linkage mapping anchored 130-Mb sequences onto the six linkage groups. A total of 10,951 complete and 19,848 partial structures of protein-encoding genes were assigned to the genome. Comparative analysis of these genes revealed the expansion of several functional domains and gene families that are characteristic of L. japonicus. Synteny analysis detected traces of whole-genome duplication and the presence of synteny blocks with other plant genomes to various degrees. This study provides the first opportunity to look into the complex and unique genetic system of legumes.
Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba, Japan.
Shusei
S
Sato
Yasukazu
Y
Nakamura
Takakazu
T
Kaneko
Erika
E
Asamizu
Tomohiko
T
Kato
Mitsuteru
M
Nakao
Shigemi
S
Sasamoto
Akiko
A
Watanabe
Akiko
A
Ono
Kumiko
K
Kawashima
Tsunakazu
T
Fujishiro
Midori
M
Katoh
Mitsuyo
M
Kohara
Yoshie
Y
Kishida
Chiharu
C
Minami
Shinobu
S
Nakayama
Naomi
N
Nakazaki
Yoshimi
Y
Shimizu
Sayaka
S
Shinpo
Chika
C
Takahashi
Tsuyuko
T
Wada
Manabu
M
Yamada
Nobuko
N
Ohmido
Makoto
M
Hayashi
Kiichi
K
Fukui
Tomoya
T
Baba
Tomoko
T
Nakamichi
Hirotada
H
Mori
Satoshi
S
Tabata
D002874:Chromosome Mapping; D018744:DNA, Plant; D020440:Gene Duplication; D017343:Genes, Plant; D018745:Genome, Plant; D017404:In Situ Hybridization, Fluorescence; D000070116:Lotus / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D017422:Sequence Analysis, DNA; D026801:Synteny
D018744:DNA, Plant; D010940:Plant Proteins