Beta-adrenergic stimulation enhances the heat-shock response in fish.
We have taken advantage of the unique properties of nucleated rainbow trout (Oncorhynchus mykiss) red blood cells (rbcs) to demonstrate that beta-adrenergic stimulation with the agonist, isoproterenol, significantly enhanced the heat-induced induction of heat-shock proteins (Hsps) in trout rbcs without affecting hsp expression on its own. Furthermore, this beta-adrenergic potentiation of hsp expression occurred only at physiologically relevant concentrations of adrenergic stimulation. In further experiments, we found that adrenaline increased 100-fold and noradrenaline increased 50-fold in trout after a 1-h heat shock at 25 degrees C, approximately 12 degrees C above acclimation temperature. This is the first time the adrenergic heat-shock response has been described for a temperate fish species. We conclude that beta-adrenergic stimulation enhances hsp expression in trout rbcs following heat stress, indicating physiological regulation of the cellular stress response in fish.
Department of Biology, Mount Allison University, Sackville, New Brunswick E4L 1G7, Canada. scurrie@mta.ca
Suzanne
S
Currie
Katharine
K
Reddin
Patrick
P
McGinn
Tom
T
McConnell
Steve F
SF
Perry
D000318:Adrenergic beta-Agonists / Q000494:pharmacology*; D000818:Animals; D004837:Epinephrine / Q000097:blood; D004900:Erythroblasts / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D018869:Heat-Shock Response / Q000187:drug effects / Q000502:physiology*; D015151:Immunoblotting; D007545:Isoproterenol / Q000494:pharmacology*; D008297:Male; D009638:Norepinephrine / Q000097:blood; D017686:Oncorhynchus mykiss / Q000097:blood / Q000502:physiology*; D012313:RNA / Q000737:chemistry / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000318:Adrenergic beta-Agonists; D006360:Heat-Shock Proteins; D012313:RNA; D007545:Isoproterenol; D009638:Norepinephrine; D004837:Epinephrine