RNA silencing-mediated resistance is related to biotic / abiotic stresses and cellular RdRp expression in transgenic tobacco plants.
The discovery of RNA silencing inhibition by virus encoded suppressors or low temperature leads to concerns about the stability of transgenic resistance. RNA-dependent RNA polymerase (RdRp) has been previously characterized to be essential for transgene-mediated RNA silencing. Here we showed that low temperature led to the inhibition of RNA silencing, the loss of viral resistance and the reduced expression of host RdRp homolog (NtRdRP1) in transgenic T4 progeny with untranslatable potato virus Y coat protein (PVY-CP) gene. Moreover, RNA silencing and the associated resistance were differently inhibited by potato virus X (PVX) and tobacco mosaic virus (TMV) infections. The increased expression of NtRdRP1 in both PVX and TMV infected plants indicated its general role in response to viral pathogens. Collectively, we propose that biotic and abiotic stress factors affect RNA silencing-mediated resistance in transgenic tobacco plants and that their effects target different steps of RNA silencing.
State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, P.R. China.
Xiao-Liang
XL
Wu
Wen-Cui
WC
Hou
Mei-Mei
MM
Wang
Xiao-Ping
XP
Zhu
Fang
F
Li
Jie-Dao
JD
Zhang
Xin-Zheng
XZ
Li
Xing-Qi
XQ
Guo
D015152:Blotting, Northern; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D030821:Plants, Genetically Modified; D017863:Potexvirus / Q000235:genetics / Q000254:growth & development; D034622:RNA Interference; D012324:RNA-Dependent RNA Polymerase / Q000235:genetics* / Q000378:metabolism; D013696:Temperature; D014026:Nicotiana / Q000235:genetics* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000235:genetics / Q000254:growth & development
D012324:RNA-Dependent RNA Polymerase