HLA-G gene polymorphism in human placentas: possible association of G*0106 allele with preeclampsia and miscarriage.
Definite causes for several pathologies of pregnancy remain unknown. In light of several recent studies, however, diminished or aberrant HLA-G expression may be associated with certain complication of pregnancy and be linked to HLA-G polymorphism. We analyzed DNA from 60 normal placentas (controls), 140 placentas from miscarriage, 36 placentas from preeclampsia, 76 placentas from fetal hypotrophy, and 34 placentas with hypoxia for variations in coding regions (allelic groups G*0101 to G*0107) and the 14-bp deletion/insertion into the 3'-untranslated region. No statistically significant differences were observed in the distribution of allelic group between pathological placentas and controls with the exception of G*0106 allele frequency in preeclamptic compared with control placentas (21.2% and 6.6%, respectively). A greater frequency of this allele also was observed in the two subgroups of miscarriage and hypoxia compared with that in controls. In addition, presence of the 14-bp sequence was prominent in preeclampsia compared with controls (60.8% vs. 35%, respectively), and homozygotes with deletion were not detected in the pathology. The results suggest that the G*0106 allele, which is coupled with the presence of the 14-bp sequence, contributes and/or is a relevant marker in some specific complications of pregnancy, especially preeclampsia.
Commissariat à l'Energie Atomique, IBM, Service de Recherches en Hémato-Immunologie, IUH, Hôpital Saint-Louis, 75475 Paris, France. philippe.moreau@cea.fr
Philippe
P
Moreau
Licinio
L
Contu
Francesco
F
Alba
Sara
S
Lai
Renata
R
Simoes
Sandro
S
Orrù
Carlo
C
Carcassi
Michel
M
Roger
Michèle
M
Rabreau
Edgardo D
ED
Carosella
D020413:3' Untranslated Regions / Q000235:genetics; D000022:Abortion, Spontaneous / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000293:Adolescent; D000328:Adult; D000483:Alleles; D015415:Biomarkers / Q000378:metabolism; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D006680:HLA Antigens / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D059951:HLA-G Antigens; D015395:Histocompatibility Antigens Class I / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D010920:Placenta / Q000378:metabolism* / Q000473:pathology; D011110:Polymorphism, Genetic; D011225:Pre-Eclampsia / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D011247:Pregnancy
D020413:3' Untranslated Regions; D015415:Biomarkers; D006680:HLA Antigens; D059951:HLA-G Antigens; C530755:HLA-G*01:06 antigen; D015395:Histocompatibility Antigens Class I