Experimental and computational approaches to quantitative proteomics: status quo and outlook.
Proteomics has come a long way from the initial qualitative analysis of proteins present in a given sample at a given time ("cataloguing") to large-scale characterization of proteomes, their interactions and dynamic behavior. Originally enabled by breakthroughs in protein separation and visualization (by two-dimensional gels) and protein identification (by mass spectrometry), the discipline now encompasses a large body of protein and peptide separation, labeling, detection and sequencing tools supported by computational data processing. The decisive mass spectrometric developments and most recent instrumentation news are briefly mentioned accompanied by a short review of gel and chromatographic techniques for protein/peptide separation, depletion and enrichment. Special emphasis is placed on quantification techniques: gel-based, and label-free techniques are briefly discussed whereas stable-isotope coding and internal peptide standards are extensively reviewed. Another special chapter is dedicated to software and computing tools for proteomic data processing and validation. A short assessment of the status quo and recommendations for future developments round up this journey through quantitative proteomics.
Panchaud
Alexandre
A
Department of Fundamental Microbiology, University of Lausanne, CH-1015 Lausanne, Switzerland.
Affolter
Michael
M
Moreillon
Philippe
P
Kussmann
Martin
M
D019295:Computational Biology / Q000379:methods / Q000639:trends; D006801:Humans; D011506:Proteins / Q000302:isolation & purification; D040901:Proteomics / Q000295:instrumentation / Q000379:methods / Q000639:trends
D011506:Proteins