Lack of association among polymorphic xenobiotic-metabolizing enzyme genotypes and the occurrence and progression of oral carcinoma in a Brazilian population.
BACKGROUND
A case control association study was carried out to investigate polymorphisms in genes CYP1A1 (3801T > C), GSTM1, and GSTT1 (null genotypes) and oral squamous cell carcinoma (OSCC), including a correlation with some histopathological findings (tumor size, lymph node invasion and degree of tumor differentiation).


METHODS
The patients (n = 91) and the controls (n = 81) were matched by age, sex, ethnicity and smoking habits. The molecular analysis was carried out using Polymerase Chain Reaction-Restrict Length Polymorphisms PCR-RFLP (CYP1A1) and Multiplex-PCR (GSTM1/GSTT1).


RESULTS
No association was found for any of the studied genes: CYP1A1 (odds ratio (OR) = 1.24; 95% Confidence Interval (CI) = 0.67-2.31), GSTM1 (OR = 0.61; CI 95% = 0.33-1.11), and GSTT1 (OR = 1.24; CI 95% = 0.65-2.38). The analysis of combining genotypes also showed lack of association. Comparison with the histopathological findings did not, in general, detect any statistically significant differences.


CONCLUSIONS
CYP1A1, GSTM1 and GSTT1 polymorphisms do not appear to influence the genetic susceptibility to OSCC or the progression to more advanced stages.
Laboratório de Citogenética Humana e Oncogenética, Universidade Federal do Paraná, Curitiba, PR, Brazil.
Roberta
R
Losi-Guembarovski
Ilce Mara de Syllos
IM
Cólus
Rodrigo Paes
RP
De Menezes
Fernando
F
Poliseli
Vivian Nappi
VN
Chaves
Hellen
H
Kuasne
Andrei
A
Leichsenring
Alda Losi
AL
Guembarovski
Benedito W
BW
Oliveira
Gyl
G
Ramos
Teresa C S
TC
Cavalcanti
Lauro Toyshi
LT
Mizuno
Iglenir João
IJ
Cavalli
Enilze Maria de Souza Fonseca
EM
Ribeiro
D001938:Brazil; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D002454:Cell Differentiation; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D018450:Disease Progression; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics* / Q000473:pathology; D009362:Neoplasm Metastasis; D011110:Polymorphism, Genetic; D015262:Xenobiotics / Q000378:metabolism*
D019363:Cytochrome P-450 CYP1A1; D005982:Glutathione Transferase; D015262:Xenobiotics; C413545:glutathione S-transferase T1