A potentiometric protein sensor built with surface molecular imprinting method.
Surface molecular imprinting, as compared to molecular imprinted bulk polymers, has the advantages of higher re-occupation percentage of the reception sites, fast response, integration of sensing element and transducer, etc. In this study, a potentiometric protein sensor was developed based on the surface molecular imprinting technique. Using the self-assembled monolayers of alkanethiol with hydroxyl terminal groups as the matrix material, and target protein molecules as the template, the sensing layer was created on the surface of the gold-coated silicon chip-an electrochemical transducer. Potentiometric measurement demonstrated that the sensor could selectively detect myoglobin or hemoglobin molecules, either with or without the presence of other protein molecules in the same solution.
Department of Materials Science and Engineering, Stony Brook University, Stony Brook, NY 11794-2275, USA.
Yantian
Y
Wang
Yanxiu
Y
Zhou
Jonathon
J
Sokolov
Basil
B
Rigas
Kalle
K
Levon
Miriam
M
Rafailovich
D015374:Biosensing Techniques / Q000379:methods*; D006454:Hemoglobins / Q000032:analysis; D054802:Molecular Imprinting / Q000379:methods*; D009211:Myoglobin / Q000032:analysis; D011199:Potentiometry / Q000379:methods*; D011506:Proteins / Q000032:analysis*
D006454:Hemoglobins; D009211:Myoglobin; D011506:Proteins