[The role of cyclin-dependent protein kinase 2 in the replication of herpes simplex virus].
Cyclin-dependent protein kinase (CDK) plays an important role in the replication of herpes simplex virus (HSV) and other important human disease viruses. But which kinds of CDK are required in the replication of HSV is still not clear. In this study, we infected dominant negative CDK2 cell line with different multiplicity of infection (MOI) of HSV-1-KOS strain (abbreviated as HSV below), and the results showed that the yield of HSV depended on the MOI; the replication of HSV delayed about 3 h as compared with that of the control in the one-step growth curve replication experiments; the CDK2 activity was induced 6 h post HSV infection and reached the highest 9 h post infection; the HSV went into rapid productive replication after the CDK2 was induced. We propose herein that the CDK2 is required in the initiation of replication of HSV.
The Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education /Inner Mongolia University, Huhhot 010021, China. guanzehong@yahoo.com.cn
Ze-Hong
ZH
Guan
Shorgen
D000818:Animals; D049109:Cell Proliferation; D002522:Chlorocebus aethiops; D051357:Cyclin-Dependent Kinase 2 / Q000502:physiology*; D019073:HT29 Cells; D006801:Humans; D018139:Simplexvirus / Q000502:physiology*; D014709:Vero Cells; D014779:Virus Replication
C495894:CDK2 protein, human; D051357:Cyclin-Dependent Kinase 2