Electroporation as an efficient method of gene transfer.
Gene transfer by electroporation is an indispensable method for the study of developmental biology, especially for the study using chick embryos. Here we briefly review the principles of the method, and its application to chick embryos. Methods of transient misexpression and long-term misexpression by retrovirus vector or transposon system, and knockdown by small interference RNA are reviewed.
Department of Molecular Neurobiology, Graduate School of Life Sciences and Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan.
Noritaka
N
Odani
Kodai
K
Ito
Harukazu
H
Nakamura
D000418:Albumins / Q000378:metabolism; D000818:Animals; D002642:Chick Embryo; D004251:DNA Transposable Elements; D018076:DNA, Complementary / Q000378:metabolism; D015509:Developmental Biology / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D020868:Gene Silencing; D018014:Gene Transfer Techniques; D005822:Genetic Vectors; D009432:Neural Crest / Q000196:embryology; D034741:RNA, Small Interfering / Q000378:metabolism; D012190:Retroviridae / Q000235:genetics
D000418:Albumins; D004251:DNA Transposable Elements; D018076:DNA, Complementary; D034741:RNA, Small Interfering