Role of lymphocytes in the course of murine zymosan-induced peritonitis.
OBJECTIVE
To investigate a putative role of lymphocytes in a murine model of zymosan peritonitis.


METHODS
Rag-deficient mice (KO) and their counterparts (WT) (13 animals in each group).


METHODS
Mice were injected i. p. with zymosan (2 mg/ml, 0.5 ml/mouse) and sacrificed either 30 min or 6 h post-treatment.


METHODS
At 30 min of inflammation vascular permeability was assessed by peritoneal leakage of i. v. injected Evans blue. At 6 h of peritonitis leukocyte numbers were estimated (Turk's staining), and MMP-2 and -9 presence (zymography). Levels of inflammatory mediators were evaluated by either ELISA (PGE(2), KC) or Cytometric Bead Array (IL-6, IL-10, MCP-1, IFN-gamma, TNF-alpha, and IL-12p70). The Amount of nitric oxide (NO) was measured by the Greiss reaction. Differences between WT and KO mice were analyzed by Student's t-test (p </=0.05).


RESULTS
During zymosan peritonitis, there was a decreased production of IFN-alpha (p = 0.03) and IL-10 (p = 0.03) and elevated synthesis of NO (p = 0.0001) in KO mice compared with WT controls. Despite this, no alterations in major events of peritonitis (vascular permeability and neutrophil infiltration) were detected in KO mice.


CONCLUSIONS
Lymphocytes do not have a significant impact on zymosan peritonitis in mice.
Department of Evolutionary Immunobiology, Institute of Zoology, Jagiellonian University, ul. Ingardena 6, PL-30-060 Krakow, Poland. ela.kolaczkowska@uj.edu.pl
E
E
Kolaczkowska
M
M
Barteczko
B
B
Plytycz
B
B
Arnold
D000818:Animals; D002199:Capillary Permeability; D018925:Chemokines / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009569:Nitric Oxide / Q000378:metabolism; D010538:Peritonitis / Q000139:chemically induced / Q000276:immunology; D040081:Protein Array Analysis; D015054:Zymosan / Q000494:pharmacology*
D018925:Chemokines; D016207:Cytokines; D018398:Homeodomain Proteins; C064658:RAG-1 protein; D009569:Nitric Oxide; D015054:Zymosan; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D015232:Dinoprostone