Neonatal pertussis presenting as acute bronchiolitis: direct detection of the Bordetella pertussis genome using loop-mediated isothermal amplification.
We report a 28-day-old female infant with pertussis presenting as severe acute bronchiolitis with cyanosis. On admission, the patient's symptoms were similar to those of acute bronchiolitis. However, occasional apneic episodes with cyanosis and peripheral lymphocytosis suggested neonatal pertussis and prompted us to examine the presence of Bordetella pertussis using loop-mediated isothermal amplification (LAMP) based on the insertion sequence IS481. LAMP of the nasopharyngeal and intratracheal aspirates was positive for B. pertussis and a diagnosis of neonatal pertussis was made. As the clinical features of pertussis in neonates and early infancy are not characteristic, LAMP is a useful tool for rapid diagnosis of B. pertussis infection.
Department of Pediatrics, National Hospital Organization, Higashi-Hiroshima Medical Center, Higashi-Hiroshima, Japan. nakamura_akari@hiro-hosp.jp
Akari
A
Nakamura
Takashi
T
Sakano
Tetsuo
T
Nakayama
Hiroko
H
Shimoda
Yasuyuki
Y
Okada
Ryuzo
R
Hanayama
Katsuhiro
K
Nomoto
Tetsushi
T
Suto
Yoshihisa
Y
Kinoshita
Takeki
T
Furue
Hiroaki
H
Ono
Toshiyuki
T
Ohta
D000208:Acute Disease; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D001988:Bronchiolitis / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D017291:Clarithromycin / Q000627:therapeutic use; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007231:Infant, Newborn; D007232:Infant, Newborn, Diseases / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D021141:Nucleic Acid Amplification Techniques / Q000379:methods; D010878:Piperacillin / Q000627:therapeutic use; D014917:Whooping Cough / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology
D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D017291:Clarithromycin; D010878:Piperacillin