The catecholaminergic RCSN-3 cell line: a model to study dopamine metabolism.
RCSN-3 cells are a cloned cell line derived from the substantia nigra of an adult rat. The cell line grows in monolayer and does not require differentiation to express catecholaminergic traits, such as (i) tyrosine hydroxylase; (ii) dopamine release; (iii) dopamine transport; (iv) norepinephrine transport; (v) monoamine oxidase (MAO)-A expression, but not MAO-B; (vi) formation of neuromelanin; (vii) VMAT-2 expression. In addition, this cell line expresses serotonin transporters, divalent metal transporter, DMT1, dopamine receptor 1 mRNA under proliferating conditions, and dopamine receptor 5 mRNA after incubation with dopamine or dicoumarol. Expression of dopamine receptors D(2), D(3) and D(4) mRNA were not detected in proliferating cells or when the cells were treated with dopamine, CuSO(4), dicoumarol or dopamine-copper complex. Angiotensin II receptor mRNA was also found to be expressed, but it underwent down regulation in the presence of aminochrome. Total quinone reductase activity corresponded 94% to DT-diaphorase. The cells also express antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase. This cell line is a suitable in vitro model for studies of dopamine metabolism, since under proliferating conditions the cells express all the pertinent markers.
Molecular and Clinical Pharmacology, ICBM, Faculty of Medicine, Casilla 70000, Santiago-7, Chile.
Irmgard
I
Paris
Jorge
J
Lozano
Sergio
S
Cardenas
Carolina
C
Perez-Pastene
Katherine
K
Saud
Patricio
P
Fuentes
Pablo
P
Caviedes
Alexies
A
Dagnino-Subiabre
Rita
R
Raisman-Vozari
Takeshi
T
Shimahara
John P
JP
Kostrzewa
David
D
Chi
Richard M
RM
Kostrzewa
Raul
R
Caviedes
Juan
J
Segura-Aguilar
D000818:Animals; D002461:Cell Line, Transformed / Q000378:metabolism / Q000648:ultrastructure; D002478:Cells, Cultured; D004298:Dopamine / Q000378:metabolism*; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000166:cytology* / Q000378:metabolism* / Q000648:ultrastructure; D050479:Neurotransmitter Transport Proteins / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D051381:Rats; D011916:Rats, Inbred F344; D013378:Substantia Nigra / Q000166:cytology; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism
D050479:Neurotransmitter Transport Proteins; D010088:Oxidoreductases; D014446:Tyrosine 3-Monooxygenase; D004298:Dopamine