Lifeact: a versatile marker to visualize F-actin.
Live imaging of the actin cytoskeleton is crucial for the study of many fundamental biological processes, but current approaches to visualize actin have several limitations. Here we describe Lifeact, a 17-amino-acid peptide, which stained filamentous actin (F-actin) structures in eukaryotic cells and tissues. Lifeact did not interfere with actin dynamics in vitro and in vivo and in its chemically modified peptide form allowed visualization of actin dynamics in nontransfectable cells.
Max Planck Institute of Biochemistry, Independent Junior Research Group Cellular Dynamics and Cell Patterning, Am Klopferspitz 18, 82152 Martinsried, Germany.
Julia
J
Riedl
Alvaro H
AH
Crevenna
Kai
K
Kessenbrock
Jerry Haochen
JH
Yu
Dorothee
D
Neukirchen
Michal
M
Bista
Frank
F
Bradke
Dieter
D
Jenne
Tad A
TA
Holak
Zena
Z
Werb
Michael
M
Sixt
Roland
R
Wedlich-Soldner
D000199:Actins / Q000378:metabolism*; D000818:Animals; D002478:Cells, Cultured; D004396:Coloring Agents / Q000737:chemistry*; D003599:Cytoskeleton / Q000378:metabolism; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051379:Mice; D008856:Microscopy, Fluorescence; D010455:Peptides / Q000737:chemistry*; D051381:Rats; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods*
D000199:Actins; D004396:Coloring Agents; D010455:Peptides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins