Novel functions of acyl-CoA thioesterases and acyltransferases as auxiliary enzymes in peroxisomal lipid metabolism.
Peroxisomes are single membrane bound organelles present in almost all eukaryotic cells, and to date have been shown to contain approximately 60 identified enzymes involved in various metabolic pathways, including the oxidation of a variety of lipids. These lipids include very long-chain fatty acids, methyl branched fatty acids, prostaglandins, bile-acid precursors and xenobiotics that are either beta-oxidized or alpha-oxidized in peroxisomes. The recent identification of several acyl-CoA thioesterases and acyltransferases in peroxisomes has revealed their various functions in acting as auxiliary enzymes in alpha- and beta-oxidation in this organelle. To date, 9 functional acyl-CoA thioesterases and acyltransferases have been identified in mouse and 4 functional acyl-CoA thioesterases and acyltransferases in human, thus these enzymes make up a substantial portion of peroxisomal proteins. This review will therefore focus on new and emerging roles for these enzymes in assisting with the oxidation of various lipids, amidation of lipids for excretion from peroxisomes, and in controlling coenzyme A levels in peroxisomes.
Hunt
Mary C
MC
Department of Genetics, Microbiology and Toxicology, Arrhenius Laboratory, Stockholm University, Svante Arrhenius väg 16F, SE-106 91 Stockholm, Sweden. mary.hunt@gmt.su.se
Alexson
Stefan E H
SE
D000217:Acyltransferases / Q000502:physiology*; D000818:Animals; D001647:Bile Acids and Salts / Q000378:metabolism; D003065:Coenzyme A / Q000378:metabolism; D003998:Dicarboxylic Acids / Q000378:metabolism; D050556:Fatty Acid-Binding Proteins / Q000502:physiology; D005227:Fatty Acids / Q000378:metabolism; D006801:Humans; D050356:Lipid Metabolism / Q000502:physiology*; D051379:Mice; D010084:Oxidation-Reduction; D020675:Peroxisomes / Q000378:metabolism*; D013869:Thiolester Hydrolases / Q000502:physiology*
D001647:Bile Acids and Salts; D003998:Dicarboxylic Acids; D050556:Fatty Acid-Binding Proteins; D005227:Fatty Acids; D000217:Acyltransferases; D013869:Thiolester Hydrolases; D003065:Coenzyme A