Enantioselective analysis of oxybutynin and N-desethyloxybutynin with application to an in vitro biotransformation study.
An enantioselective method using liquid-phase microextraction (LPME) followed by HPLC analysis was developed for the determination of oxybutynin (OXY) and its major metabolite N-desethyloxybutynin (DEO) in rat liver microsomal fraction. The LPME procedure was optimized using multifactorial experiments. Under the optimal extraction conditions, the mean recoveries were 61 and 55% for (R)-OXY and (S)-OXY, respectively, and 70 and 76% for (R)-DEO and (S)-DEO, respectively. The validated method was employed to an in vitro biotransformation study using rat liver microsomal fraction. The results demonstrated the enantioselective biotransformation of OXY.
da Fonseca
Patrícia
P
Faculdade de Ciências Farmacâeuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.
de Freitas
Luis Alexandre Pedro
LA
Pinto
Luis Felipe Ribeiro
LF
Pestana
Cezar Rangel
CR
Bonato
Pierina Sueli
PS
D000818:Animals; D001711:Biotransformation; D005591:Chemical Fractionation / Q000379:methods; D008297:Male; D008333:Mandelic Acids / Q000032:analysis* / Q000493:pharmacokinetics; D008862:Microsomes, Liver / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013237:Stereoisomerism
D008333:Mandelic Acids; C032681:desethyloxybutynin; C005419:oxybutynin