[Clonal transmission of methicillin-resistant S. aureus (MRSA) strain in two surgical wards in Lódź].
OBJECTIVE
Phenotypic and genotypic characteristics of S. aureus strains isolated from selected operative wards.


METHODS
A group of 50 strains were analysed. Bacterial strains were isolated from three operative wards of Pirogow Specialistic Hospital in Lódź in a year. Biochemical properties and drug resistance were estimated using standard microbiological techniques. PCR MP technique was used for genotypic analysis.


METHODS
A convergence of genotyping results and antibiotic resistance profiles among MRSA strains was observed. Thus, the antibiogram may serve as the instrument for the preliminary typing of S. aureus strains that may be related. However, this hypothesis has to be verified, for example by using PCR MP technique.


RESULTS
Methicillin-resistance was demonstrated in 24 out of 50 S. aureus isolates. Most MRSA strains (19 out of 24) presented multidrugresistance. Twelve various profiles of enzymatic activity and fifteen different genotypes (A-O) were singled out among the investigated strains. Profile A, recorded in 20 strains, was most frequent (Dice Coefficient: 0.80-0.94).


CONCLUSIONS
1. S. aureus strains present high resistance to antibiotics frequently showing multidrug-resistance coupled with methicillin-resistance 2. Genetically related MRSA strains present a stable drug-resistance pattern 3. Clonal transmission of MRSA strain was observed in two operative wards 4. The disc-diffusion method for testing antibiotic-resistance and PCR MP technique are useful in epidemiological investigation for S. aureus.
Zakład Mikrobiologii Lekarskiej i Sanitarnej Katedry Mikrobiologii UM w Lodzi. microbiology@op.pl
Monika
M
Broda
Ireneusz
I
Ciebiada
Andrzej
A
Denys
D015373:Bacterial Typing Techniques / Q000379:methods*; D003428:Cross Infection / Q000382:microbiology*; D018432:Drug Resistance, Multiple; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006779:Hospitals, Public; D006801:Humans; D016106:Methicillin Resistance / Q000235:genetics*; D011044:Poland; D016133:Polymerase Chain Reaction; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000235:genetics*; D013503:Surgery Department, Hospital