Moments of discovery.
Devoted teachers and mentors during early childhood and adolescence nurtured my ambition to become a scientist, but it was not until I actually began doing experiments in college and graduate school that I was confident about that choice and of making it a reality. During my postdoctoral experiences and thereafter, I made several significant advances, most notably the discovery of the then novel acyl- and aminoacyl adenylates: the former as intermediates in fatty acyl coenzyme A (CoA) formation and the latter as precursors to aminoacyl tRNAs. In the early 1970s, my research changed from a focus on transcription and translation in Escherichia coli to the molecular genetics of mammalian cells. To that end, my laboratory developed a method for creating recombinant DNAs that led us and others, over the next two decades, to create increasingly sophisticated ways for introducing "foreign" DNAs into cultured mammalian cells and to target modifications of specific chromosomal loci. Circumstances surrounding that work drew me into the public policy debates regarding recombinant DNA practices. As an outgrowth of my commitment to teaching, I co-authored several textbooks on molecular genetics and a biography of George Beadle. The colleagues, students, and wealth of associates with whom I interacted have made being a scientist far richer than I can have imagined.
Cancer Research and Biochemistry, Stanford University Medical Center, Stanford, CA 94305-5020, USA. pberg@stanford.edu
Paul
P
Berg
D000818:Animals; D001671:Biochemistry / Q000266:history*; D005822:Genetic Vectors; D049673:History, 20th Century; D049674:History, 21st Century; D006801:Humans; D011506:Proteins / Q000378:metabolism; D013539:Simian virus 40 / Q000378:metabolism; D014481:United States
D011506:Proteins