IFN-inducible protein 10 (CXCL10) regulates tubular cell proliferation in renal ischemia-reperfusion injury.
Although renal tubular cell proliferation after acute tubular necrosis is an important and essential response in the recovery of renal dysfunction in acute renal failure, the precise factors and mechanisms of tubular cell regeneration remain unclear. Here, we describe our studies using a neutralizing antibody (Ab) against interferon-inducible protein of 10 kDa (IP-10; CXCL10) that indicate a role for CXCL10 in tubular cell proliferation after renal ischemia-reperfusion injury. Tissue necrosis and interstitial infiltrating numbers were comparable between anti-CXCL10 Ab-treated and control mice treated with IgG at the 24 and 48 h time points after reperfusion. In contrast, the numbers of Ki67-positive proliferating tubular cells were significantly increased in anti-CXCL10 Ab-treated mice 48 h after reperfusion. In accordance with the in vivo findings,in vitro studies using murine tubular epithelial cells indicated an antiproliferative effect of CXCL10 upon the intensity of cell proliferation and the number of Ki67-positive cells. These data suggest that CXCL10 plays a role in the regulation of tubular cell proliferation following renal ischemia-reperfusion injury.
Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Science, Kanazawa, Japan. kfuruichi@m-kanazawa.jp
Kengo
K
Furuichi
Takashi
T
Wada
Shinji
S
Kitajikma
Tadasi
T
Toyama
Toshiya
T
Okumura
Akinori
A
Hara
Hiroshi
H
Kawachi
Fujio
F
Shimizu
Takeshi
T
Sugaya
Naofumi
N
Mukaida
Shosaku
S
Narumi
Kouji
K
Matsushima
Shuichi
S
Kaneko
D000818:Animals; D049109:Cell Proliferation; D054357:Chemokine CXCL10 / Q000378:metabolism*; D005246:Feedback; D007673:Kidney Cortex Necrosis / Q000378:metabolism* / Q000473:pathology*; D007684:Kidney Tubules / Q000378:metabolism* / Q000473:pathology*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology*
D054357:Chemokine CXCL10; C485433:Cxcl10 protein, mouse