A rapid method for determining dynamic binding capacity of resins for the purification of proteins.
Biolayer interferometry is a novel method for quantifying macromolecules, such as proteins, in solution. The presence of other, non-binding molecules does not interfere with quantification, which allows one to measure the concentration of the molecule of interest in a crude mixture. Here we apply this method to determining the dynamic binding capacity of affinity resins.
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Tri
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Raven Biotechnologies, Inc. Manufacturing, One Corporate Drive, South San Francisco, CA 94080, USA.
Ho
Frank
F
Heidecker
Bettina
B
Witte
Krista
K
Chang
Lucille
L
Lerner
Laura
L
D000818:Animals; D016466:CHO Cells; D002851:Chromatography, High Pressure Liquid; D006224:Cricetinae; D003412:Cricetulus; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D011485:Protein Binding; D011506:Proteins / Q000302:isolation & purification* / Q000378:metabolism
D011506:Proteins