Use of commercially available rabbit monoclonal antibodies for immunofluorescence double staining.
Immunohistochemistry, that is, the use of polyclonal and monoclonal antibodies to detect cell and tissue antigens at a microscopical level is a powerful tool for both research and diagnostic purposes. Especially in the field of hematologic disease, there is often a need to detect several antigens synchronously, and we report here a fast and easy technique for demonstrating more than 1 antigen in 1 slide using immunofluorescence. We have used commercially available rabbit monoclonal antibodies (Cyclin D1, CD3, CD5, CD23, etc.) paired with mouse monoclonal antibodies (CD7, CD20, CD79a, Pax-5, etc.) for double immunofluorescence labeling on paraffin-embedded tissue sections. Commercially available rabbit monoclonal antibodies in combination with mouse monoclonal antibodies proved useful in double immunofluorescence labeling on paraffin-embedded tissue, and all combinations used yielded excellent results.
Department of Pathology, Naestved Hospital, Region Sjaelland, Ringstedgade 61, Naestved 4700, Denmark. mbz@cn.stam.dk
Michael
M
Bzorek
Inger Merete
IM
Stamp
Bodil Laub
BL
Petersen
Lisbeth
L
Frederiksen
D000818:Animals; D000911:Antibodies, Monoclonal; D005455:Fluorescent Antibody Technique; D006041:Goats; D006801:Humans; D051379:Mice; D016612:Paraffin Embedding; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000175:diagnosis / Q000473:pathology; D011817:Rabbits; D013194:Staining and Labeling
D000911:Antibodies, Monoclonal