Coenzyme Q_{10} effects on manganese superoxide dismutase and glutathione peroxidase in the hairless mouse skin induced by ultraviolet B irradiation.
Coenzyme Q_{10} (CoQ_{10}) is a naturally occurring antioxidant and a prominent component of mitochondrial electron transport chain. In the present study, we investigated the effect of CoQ_{10} nanoparticle against photoaging using immunohistochemistry and Western blot analysis in the hairless mouse skin induced by ultraviolet B (UVB) irradiation (300 mJ/cm;{2}, 3 min/day for 21 days). In the UVB-irradiated distilled water (DW)-treated group, manganese superoxide dismutase (SOD2) and glutathione peroxidase (GPx) immunoreactivity and their protein levels in the skin were significantly lower than those in the control group. However, SOD2 and GPx immunoreactivity and their protein levels in the skin of the UVB-irradiated CoQ_{10}-treated group were higher than those in the UVB-irradiated DW-treated group. GPx activity in the skin in the UVB-irradiated DW-treated group significantly decreased compared to that in the control group; whereas GPx activity in the UVB-irradiated CoQ_{10}-treated group was similar to that in the control group. These results suggest that CoQ_{10} strongly inhibits oxidative stress in the skin induced by UVB via increasing SOD2 and GPx.
Kim
Dong-Woo
DW
Research Center, Natural F&P Co., Ltd, Chuncheon, South Korea.
Hwang
In Koo
IK
Kim
Dae Won
DW
Yoo
Ki-Yeon
KY
Won
Chung-Kil
CK
Moon
Won-Kook
WK
Won
Moo-Ho
MH
D000818:Animals; D015153:Blotting, Western; D004789:Enzyme Activation / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism*; D007150:Immunohistochemistry; D051379:Mice; D008812:Mice, Hairless; D011897:Random Allocation; D012867:Skin / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D013482:Superoxide Dismutase / Q000378:metabolism*; D014451:Ubiquinone / Q000494:pharmacology*; D014466:Ultraviolet Rays
D014451:Ubiquinone; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase