In vitro testing of cellular response to ultra high frequency electromagnetic field radiation.
The aim of this study was to evaluate whether low-level, ultra high frequency (UHF) irradiation of 935 MHz influences the cell structure and growth of V79 cells. UHF field was generated inside a Gigahertz Transversal Electromagnetic Mode cell (GTEM-cell) with a Hewlett-Packard signal generator. The electric field strength was 8.2+/-0.3 V/cm and the average specific absorption rate (SAR) was calculated to be 0.12 W/kg. Cell samples were cultivated in a humidified atmosphere at 37 degrees C with 5% CO2. Prepared cell samples were exposed to a 935 MHz continuous wave frequency field for 1, 2, and 3 h. The structure of microtubule proteins has been determined using the immunocytochemical method. Cell growth was determined by cell counts for each hour of exposure during five post-exposure days. Negative- and positive-cell controls were included into the experimental procedure. In comparison with control cells, the microtubule structure clearly altered after 3h of irradiation (p<0.05). Significantly decreased growth was noted in cells exposed for 3h three days after irradiation (p<0.05). It seems that the 935 MHz, low-level UHF radiation affects microtubule proteins, which consequently may obstruct cell growth.
Institute for Medical Research and Occupational Health, Ksaverska cesta 2, HR-10001 Zagreb, Croatia. ipavicic@imi.hr
Ivan
I
Pavicic
Ivancica
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Trosic
D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004574:Electromagnetic Fields; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*; D008168:Lung / Q000166:cytology; D008868:Microtubule Proteins / Q000378:metabolism / Q000528:radiation effects; D008870:Microtubules / Q000528:radiation effects; D013997:Time Factors
D008868:Microtubule Proteins