[Expression of Twist in papillary thyroid carcinomas and its roles in differential diagnosis].
OBJECTIVE
To study Twist expression in thyroid papillary carcinoma (PTC) by immunohistochemistry and to assess its usefulness as marker in the differential diagnosis of PTC, follicular adenomas (FA) and benign papillary lesions (BPL).


METHODS
Fifty cases of PTC, 48 cases of FA and 47 cases of BPL were evaluated using manual tissue chip and SP immunohistochemical stain to detect the expression of Twist and HBME-1, and comparing the staining to that of cytokeratin 19 (CK19).


RESULTS
In PTC, positive rates of Twist, HBME-1 and CK19 were 100% (48/48), 94.0% (47/50) and 78.0% (39/ 50) respectively; in FA, positive rates were 0, 6.7% (3/45) and 0 respectively; in BPL, positive rates were 7.0% (3/34), 2.1% (1/47) and 0, respectively. The differences between PTC and FA and between PTC and BPL were both statistically significant (P = 0. 000). The sensitivity of Twist, HBME-1 and CK19 was 100%, 94.0% and 78.0%; the specifity was 96.4%, 95.7% and 100%; overall accurary was 97.7%, 95.1% and 91.9%, respectively.


CONCLUSIONS
Positive rates of Twist is higher than the other markers in PTC. Immunohistochemical staining of Twist has important significance in the differential diagnosis of thyroid lesions. Twist immunohistochemistry maybe helpful in diagnosis and differential diagnosis of PTC.
Department of Pathology, Hebei Provincial People's Hospital, Shijiazhuang 050051, China. hechn2@163.com
Chun-Nian
CN
He
Lili
L
He
Jin Q
JQ
Cheng
Shi-Chao
SC
Chen
Huan-Fen
HF
Zhao
Jin-Ping
JP
Zhai
Jing
J
Zhang
D018263:Adenocarcinoma, Follicular / Q000378:metabolism; D000231:Adenocarcinoma, Papillary / Q000473:pathology; D000236:Adenoma / Q000175:diagnosis / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000276:immunology; D002291:Carcinoma, Papillary / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D018265:Carcinoma, Papillary, Follicular / Q000378:metabolism*; D003937:Diagnosis, Differential; D037502:Galectin 3 / Q000235:genetics / Q000378:metabolism; D007150:Immunohistochemistry; D053539:Keratin-19 / Q000235:genetics; D007633:Keratins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D016606:Thyroid Nodule / Q000473:pathology; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism*; D000951:Antigens, Neoplasm
D014408:Biomarkers, Tumor; D037502:Galectin 3; D053539:Keratin-19; D007633:Keratins; D009687:Nuclear Proteins; D051879:Twist-Related Protein 1; C432845:HBME-1 antigen; D000951:Antigens, Neoplasm; C103173:TWIST1 protein, human