Autoregulation of AbsB (RNase III) expression in Streptomyces coelicolor by endoribonucleolytic cleavage of absB operon transcripts.
The Streptomyces coelicolor absB gene encodes an RNase III family endoribonuclease and is normally essential for antibiotic biosynthesis. Here we report that AbsB controls its own expression by sequentially and site specifically cleaving stem-loop segments of its polycistronic transcript. Our results demonstrate a ribonucleolytic regulatory role for AbsB in vivo.
Department of Genetics, MC5120, Stanford University School of Medicine, Stanford, California 94305, USA.
Weijing
W
Xu
Jianqiang
J
Huang
Stanley N
SN
Cohen
D001426:Bacterial Proteins / Q000096:biosynthesis*; D001483:Base Sequence; D015964:Gene Expression Regulation, Bacterial; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D012329:RNA, Bacterial / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D043244:Ribonuclease III / Q000096:biosynthesis*; D048372:Streptomyces coelicolor / Q000502:physiology*
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D043244:Ribonuclease III