[Genome cloning and sequence analysis of duck circovirus].
To reveal the molecular biological characteristics of genome of circovirus in infected ducks, two nucleotide fragments were amplified by overlapping PCRs using DNA extracted from various tissues of ducks. After they had been assembled together, the nucleotide components, the genome organization and the phylogenetic scale of the sequence were analyzed. The results showed that the obtained sequence is a circular DNA with a total length of 1995nt. It contains 6 open reading frames (ORFs), and shares a high identity of 97.4% with the MuDCV circovirus sequence presented in GenBank (AY228555). These results indicate that the amplified product stems from duck circovirus sequence.
Fu
Guang-Hua
GH
Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China.
Cheng
Long-Fei
LF
Shi
Shao-Hua
SH
Peng
Chun-Xiang
CX
Chen
Hong-Mei
HM
Huang
Yu
Y
D000818:Animals; D001483:Base Sequence; D017925:Circovirus / Q000145:classification / Q000235:genetics*; D003001:Cloning, Molecular; D004372:Ducks / Q000821:virology*; D014644:Genetic Variation; D008969:Molecular Sequence Data; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA