Quantitative characterization of nonstructural carbohydrates of mezcal Agave (Agave salmiana Otto ex Salm-Dick).
Fructans are the reserve carbohydrates in Agave spp. plants. In mezcal factories, fructans undergoes thermal hydrolysis to release fructose and glucose, which are the basis to produce this spirit. Carbohydrate content determines the yield of the final product, which depends on plant organ, ripeness stage, and thermal hydrolysis. Thus, a qualitative and quantitative characterization of nonstructural carbohydrates was conducted in raw and hydrolyzed juices extracted from Agave salmiana stems and leaves under three ripeness stages. By high-performance liquid chromatography (HPLC), fructose, glucose, sucrose, xylose, and maltose were identified in agave juice. Only the plant fraction with hydrolysis interaction was found to be significant in the glucose concentration plant. Interactions of the fraction with hydrolysis and ripeness with hydrolysis were statistically significant in fructose concentration. Fructose concentration rose considerably with hydrolysis, but only in juice extracted from ripe agave stems (early mature and castrated). This increase was statistically significant only with acid hydrolysis.
Michel-Cuello
Christian
C
Programa Multidisciplinario de Posgrado en Ciencias Ambientales, Instituto de Investigacion de Zonas Deserticas y Facultad de Ingenieria, Universidad Autonoma de San Luis Potosi, Altair 200, Colonia del Llano, San Luis Potosi, SLP, Mexico 78377.
Juárez-Flores
Bertha Irene
BI
Aguirre-Rivera
Juan Rogelio
JR
Pinos-Rodríguez
Juan Manuel
JM
D027882:Agave / Q000737:chemistry* / Q000254:growth & development; D002241:Carbohydrates / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D006358:Hot Temperature; D006868:Hydrolysis; D018515:Plant Leaves / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry
D002241:Carbohydrates; D005632:Fructose; D005947:Glucose