Physiological and oenological traits of different Dekkera/Brettanomyces bruxellensis strains under wine-model conditions.
Contamination of wine by Dekkera/Brettanomyces bruxellensis is mostly due to the production of off-flavours identified as vinyl- and especially ethyl-phenols, but these yeasts can also produce several other spoiling metabolites, such as acetic acid and biogenic amines. Little information is available about the correlation between growth, viability and off-flavour and biogenic amine production. In the present work, five strains of Dekkera bruxellensis isolated from wine were analysed over 3 months in wine-like environment for growth, cell survival, carbon source utilization and production of volatile phenols and biogenic amines. Our data indicate that the wine spoilage potential of D. bruxellensis is strain dependent, being strictly associated with the ability to grow under oenological conditions. 4-Ethyl-phenol and 4-ethyl-guaiacol production ranged between 0 and 2.7 and 2 mg L(-1), respectively, depending on the growth conditions. Putrescine, cadaverine and spermidine were the biogenic amines found.
Dipartimento di Scienze e Tecnologie Alimentari e Microbiologiche, Università degli Studi di Milano, Milan, Italy.
Ileana
I
Vigentini
Andrea
A
Romano
Concetta
C
Compagno
Annamaria
A
Merico
Francesco
F
Molinari
Antonio
A
Tirelli
Roberto
R
Foschino
Gaspare
G
Volonterio
D015317:Biogenic Polyamines / Q000378:metabolism*; D002244:Carbon / Q000378:metabolism; D003470:Culture Media; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D006139:Guaiacol / Q000031:analogs & derivatives* / Q000378:metabolism; D010636:Phenols / Q000378:metabolism*; D004718:Saccharomycetales / Q000145:classification / Q000254:growth & development / Q000378:metabolism / Q000502:physiology; D027843:Vitis / Q000382:microbiology; D014835:Volatilization; D014920:Wine / Q000382:microbiology*
D015317:Biogenic Polyamines; D003470:Culture Media; D010636:Phenols; D000431:Ethanol; D006139:Guaiacol; D002244:Carbon; C042291:4-ethylphenol; C084991:4-ethylguaiacol