Quality of embryonic bodies and seeding density effects on neural differentiation of mouse embryonic stem cells.
Mouse embryonic stem (ES) cells can be differentiated into neural lineage cells, but the differentiation efficiency remains low. This study revealed two important factors that influence the neural differentiation efficiency of mouse ES cells: the first is the quality of embryonic bodies (EBs); good quality of EBs consistently originated from a suspension culture of 1x10(5) ES cells/ml serum-free chemically defined neural inducing medium and they exhibited a smooth round shape, with a dark central region surrounded by a light band. Such EBs are capable of attaining high neural differentiation efficiency. However, poor quality EBs originated from a suspension culture of 1x10(6) ES cells/ml serum-free chemically defined neural inducing medium and exhibited an irregular shape or adhered to the bottom of the dish; they displayed low neural differentiation efficiency. The second factor is the seeding density of EBs: a low seeding density (5 EBs/cm2) induced cells to differentiate into a more caudalized subtypes compared to the cells obtained from high seeding density (20 EBs/cm2). These findings provided fresh insight into the neural induction of mouse ES cells.
Center for Developmental Biology, Department of Urology, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, 1665 Kong Jian Road, Shanghai 200092, PR China.
Jun-Mei
JM
Zhou
Feng-Ying
FY
Xing
Jian-Jun
JJ
Shi
Zhen-Fu
ZF
Fang
Xue-Jin
XJ
Chen
Fang
F
Chen
D000818:Animals; D019208:Brain-Derived Neurotrophic Factor / Q000494:pharmacology; D002452:Cell Count; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D019070:Cell Lineage / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D004622:Embryo, Mammalian / Q000166:cytology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000187:drug effects; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000187:drug effects; D013997:Time Factors
D019208:Brain-Derived Neurotrophic Factor