Expression of oncostatin M in chronic obstructive sialadenitis of the submandibular gland.
OBJECTIVE
We investigated the expression of oncostatin M messenger RNA (mRNA) and protein in normal submandibular glands and those with chronic obstructive sialadenitis and localized the expression of oncostatin M protein.


METHODS
Submandibular glands from 10 patients with chronic obstructive sialadenitis as a study group and 10 normal submandibular glands as a control group were examined. Oncostatin M mRNA extracted from submandibular gland was used for reverse transcription-polymerase chain reaction and analyzed semiquantitatively. The difference in expression level of oncostatin M protein between the 2 groups was analyzed through Western blot analysis, and oncostatin M protein was localized immunohistochemically.


RESULTS
The expression levels of oncostatin M mRNA and protein were significantly increased in the study group. The protein was predominantly localized in ductal epithelia and infiltrating inflammatory cells and was more strongly expressed in the study group also.


CONCLUSIONS
Oncostatin M is expressed in both chronic obstructive sialadenitis and normal submandibular gland, and is up-regulated in chronic obstructive sialadenitis. These results suggest that oncostatin M is involved in the pathologic process of chronic obstructive sialadenitis. However, the physiologic role in normal glands, as well as a possible role in the development of chronic obstructive sialadenitis, remains to be elucidated.
Lee
Heung-Man
HM
Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Korea.
Cho
Jae-Goo
JG
Kang
Hee-Joon
HJ
Chae
Sung-Won
SW
Hwang
Soon-Jae
SJ
Jung
Kwang-Yoon
KY
Woo
Jeong-Soo
JS
D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D015153:Blotting, Western; D002908:Chronic Disease; D003251:Constriction, Pathologic; D005260:Female; D005500:Follow-Up Studies; D015870:Gene Expression; D020022:Genetic Predisposition to Disease; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D053683:Oncostatin M / Q000096:biosynthesis / Q000235:genetics*; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012793:Sialadenitis / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D013363:Submandibular Gland / Q000378:metabolism* / Q000473:pathology
D015415:Biomarkers; D012333:RNA, Messenger; D053683:Oncostatin M