Serological reactivity of different antigenic preparations of Leishmania (Leishmania) amazonensis and the Leishmania braziliensis complex.
Total antigen from Leishmania (Leishmania) amazonensis and isolates from the Leishmania braziliensis complex, along with their respective antigenic fractions obtained by affinity chromatography on concanavalin-A-Sepharose and jacalin-agarose columns evaluated using immunoenzymatic ELISA assay. For this, serum samples from 229 patients were used, grouped as American tegmental leishmaniasis (n masculine=58), visceral leishmaniasis (n masculine=28), Chagas disease (n masculine=49), malaria (n masculine=32), tuberculosis (n masculine=13) and healthy volunteers (n masculine=49). Samples from American tegmentary leishmaniasis showed higher reactivity with antigens isolated from the Leishmania braziliensis complex than with antigens from Leishmania amazonensis (p<0.001). ELISA assays showed a sensitivity range from 60% to 95% with antigens isolated from the Leishmania braziliensis complex. There was marked nonspecific reactivity among serum samples with the use of antigenic fractions binding with concanavalin-A and jacalin from both Leishmania complexes, in comparison with other antigens (p<0.001). The results presented in this study suggest that the use of homologous antigens increases the efficiency of anti-Leishmania immunoglobulin detection, which may be very valuable for diagnostic purposes.
Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG.
Adriano
A
Gomes-Silva
Maria Aparecida
MA
Souza
Sandra Regina
SR
Afonso-Cardoso
Lívia Resende
LR
Andrade
Reynaldo
R
Dietze
Elenice
E
Lemos
Alejandro
A
Belli
Silvio
S
Favoreto Júnior
Marcelo Simão
MS
Ferreira
D000818:Animals; D000947:Antigens, Helminth / Q000302:isolation & purification; D016022:Case-Control Studies; D014355:Chagas Disease / Q000276:immunology; D002846:Chromatography, Affinity; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007892:Leishmania braziliensis / Q000276:immunology*; D007894:Leishmania mexicana / Q000276:immunology*; D007897:Leishmaniasis, Mucocutaneous / Q000175:diagnosis* / Q000276:immunology; D007898:Leishmaniasis, Visceral / Q000175:diagnosis* / Q000276:immunology; D008288:Malaria / Q000276:immunology; D037121:Plant Lectins; D012680:Sensitivity and Specificity; D012685:Sepharose / Q000031:analogs & derivatives; D014376:Tuberculosis / Q000276:immunology
D000947:Antigens, Helminth; D037121:Plant Lectins; C028326:concanavalin A-sepharose; C043696:jacalin; D012685:Sepharose