Overexpression of human CUTA isoform2 enhances the cytotoxicity of copper to HeLa cells.
Copper, an essential transient element, can be toxic to cells when present in excess. Altered copper homeostasis is involved in pathological events of many diseases. Human CUTA isoform2 is a member of cation tolerance protein (CutA1) family. In this study, we examined the effect of CUTA isoform2 overexpression on copper toxicity. It was shown that overexpressed CUTA isoform2 sensitized HeLa cells to copper toxicity by promoting copper-induced apoptosis. The inhibition effect of excessive copper on cell proliferation was also enhanced by overexpressed CUTA isoform2. So CUTA isoform2 was implicated to be involved in the cytotoxicity of copper.
State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University, Shanghai, P. R. China.
Jingchun
J
Yang
Qiang
Q
Li
Huirong
H
Yang
Lichong
L
Yan
Liu
L
Yang
Long
L
Yu
D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D001483:Base Sequence; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000633:toxicity*; D017931:DNA Primers / Q000235:genetics; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism*; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism
C416667:CUTA protein, human; D017931:DNA Primers; D008565:Membrane Proteins; D020033:Protein Isoforms; D003300:Copper