Phenotypic and genetic analysis of the Triticum monococcum-Mycosphaerella graminicola interaction.
Here, the aim was to understand the cellular and genetic basis of the Triticum monococcum-Mycosphaerella graminicola interaction. Testing for 5 yr under UK field conditions revealed that all 24 T. monococcum accessions exposed to a high level of natural inocula were fully resistant to M. graminicola. When the accessions were individually inoculated in the glasshouse using an attached leaf seeding assay and nine previously characterized M. graminicola isolates, fungal sporulation was observed in only three of the 216 interactions examined. Microscopic analyses revealed that M. graminicola infection was arrested at four different stages post-stomatal entry. When the inoculated leaves were detached 30 d post inoculation and incubated at 100% humidity, abundant asexual sporulation occurred within 5 d in a further 61 interactions. An F(2) mapping population generated from a cross between T. monococcum accession MDR002 (susceptible) and MDR043 (resistant) was inoculated with the M. graminicola isolate IPO323. Both resistance and in planta fungal growth were found to be controlled by a single genetic locus designated as TmStb1 which was linked to the microsatellite locus Xbarc174 on chromosome 7A(m). Exploitation of T. monococcum may provide new sources of resistance to septoria tritici blotch disease.
Jing
Hai-Chun
HC
Department of Plant Pathology and Microbiology, Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.
Lovell
Darren
D
Department of Plant Pathology and Microbiology, Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.
Gutteridge
Richard
R
Department of Plant Pathology and Microbiology, Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.
Jenk
Daniel
D
Department of Plant Pathology and Microbiology, Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.
Kornyukhin
Dmitry
D
Department of Plant Pathology and Microbiology, Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.|NI Vavilov Research Institute of Plant Industry, St Petersburg, RU-190000, Russian Federation.
Mitrofanova
Olga P
OP
NI Vavilov Research Institute of Plant Industry, St Petersburg, RU-190000, Russian Federation.
Kema
Gert H J
GHJ
Plant Research International B. V., PO Box 16, NL-6700 AA Wageningen, the Netherlands.
Hammond-Kosack
Kim E
KE
Department of Plant Pathology and Microbiology, Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.
D001203:Ascomycota / Q000502:physiology* / Q000648:ultrastructure; D017343:Genes, Plant / Q000502:physiology*; D007113:Immunity, Innate / Q000235:genetics; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D018515:Plant Leaves / Q000235:genetics / Q000382:microbiology / Q000648:ultrastructure; D011123:Polyploidy; D040641:Quantitative Trait Loci; D013172:Spores, Fungal / Q000502:physiology; D014908:Triticum / Q000235:genetics / Q000382:microbiology* / Q000648:ultrastructure