Optimization of octreotide PEGylation by monitoring with fast reversed-phase high-performance liquid chromatography.
The purpose of this study was to develop a fast reversed-phase high-performance liquid chromatography (HPLC) method for monitoring the octreotide PEGylation reaction in order to find optimal conditions for the production of the desired mono-PEGylated octreotide. The fast HPLC method could separate the positional isomers of two mono-PEGylated octreotides, di-PEGylated octreotide, and unmodified octreotide within 4.5 min. The PEGylation pattern was monitored at various pH conditions and molar ratios of reactants to allow optimization of the PEGylation reaction conditions for the production of N-terminally mono-PEGylated octreotide.
Park
Eun Ji
EJ
College of Pharmacy, Kyungsung University, 314-79 Daeyeon-dong, Nam-ku, Busan 608-736, South Korea.
Na
Dong Hee
DH
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D015282:Octreotide / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D013997:Time Factors
D011092:Polyethylene Glycols; D015282:Octreotide