Life span alteration after irradiation in Drosophila melanogaster strains with mutations of Hsf and Hsps.
The life span alteration after gamma-irradiation and/or paraquat treatment in Drosophila in wild type strain Canton-S and strains with mutations of heat shock factor (1-4 alleles) and heat shock proteins (Hsp70Ba ( 304 ), Hsp83 ( e6A ), Hsp22 ( EY09909 ), Hsp67Bb ( EY099099 )) was investigated. Chronic low-dose rate gamma-irradiation (0.017 and 0.17 cGy/h) on pre-imago stages was used as a priming dose (absorbed doses were 4 and 40 cGy). Paraquat, a free radical inducing agent, was a challenging factor (20 mM for 1 day). It was shown that chronic irradiation led to adaptive response in both sexes except homozygous males and females with mutations of Hsf ( 4 ) and Hsp70Ba ( 304 ). The gender-specific differences in stress response were discovered in wild type strain Canton-S, Hsp22 ( EY09909 ) Hsp67Bb ( EY09909 ) homozygotes and Hsp83 ( e6A ) heterozygotes: the adaptive response persisted in males, but not in females. Thus, Drosophila Hsp and Hsf mutation homozygotes did not demonstrate the adaptive response in the majority of cases, implying an important role of those genes in radiation hormesis and adaptation to stresses.
Department of Radioecology, Institute of Biology, Komi Science Center, Syktyvkar, Russia. amoskalev@list.ru
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Moskalev
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Shaposhnikov
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Turysheva
D000222:Adaptation, Physiological; D000818:Animals; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D004307:Dose-Response Relationship, Radiation; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000187:drug effects / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D005260:Female; D005720:Gamma Rays; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins / Q000235:genetics* / Q000378:metabolism; D008136:Longevity / Q000187:drug effects / Q000235:genetics / Q000528:radiation effects*; D008297:Male; D009154:Mutation; D010269:Paraquat / Q000633:toxicity; D012737:Sex Factors; D013312:Stress, Physiological / Q000235:genetics*; D013997:Time Factors; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism
D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D000076249:Heat Shock Transcription Factors; D006360:Heat-Shock Proteins; C053134:Hsf protein, Drosophila; C490752:Hsp22 protein, Drosophila; C089272:Hsp83 protein, Drosophila; D014157:Transcription Factors; D010269:Paraquat