Expression of ethylene-forming enzyme (EFE) of Pseudomonas syringae pv. glycinea in Trichoderma viride.
The efe gene encoding an ethylene-forming enzyme from Pseudomonas syringae pv. glycinea has been expressed for the first time under the control of Trichoderma reesei cbh1 promoter in Trichoderma viride. Reverse transcription polymerase chain reaction analysis showed that transformant Y2 produced mRNA of the efe gene. Southern blot analysis showed that there was one copy of efe gene which was integrated into the chromosomal DNA of T. viride. Ethylene production by transformant Y2 was efficiently induced by cellulose, while very low level of ethylene was produced when sodium carboxymethyl cellulose or lactose was used as carbon source. Peptone exerted a much greater stimulatory effect on ethylene production. A high level of ethylene was produced when transformant Y2 was cultured in solid fermentation medium containing wheat straw, indicating that plant wastes could be directly converted to ethylene by the recombinant filamentous fungus.
National Key Laboratory for Agrobiotechnology, College of Biological Sciences and Center for Biomass Engineering, China Agricultural University, Beijing, 100094, People's Republic of China.
Li
L
Tao
Hong-Jun
HJ
Dong
Xi
X
Chen
San-Feng
SF
Chen
Tian-Hong
TH
Wang
D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D005030:Ethylenes / Q000378:metabolism; D005285:Fermentation; D015870:Gene Expression; D008190:Lyases / Q000235:genetics* / Q000378:metabolism; D010461:Peptones / Q000378:metabolism; D011401:Promoter Regions, Genetic; D044224:Pseudomonas syringae / Q000201:enzymology*; D014170:Transformation, Genetic; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism
D001426:Bacterial Proteins; D005030:Ethylenes; D010461:Peptones; C036216:ethylene; D008190:Lyases; C034693:ethylene forming enzyme