Protein manipulation with insulator-based dielectrophoresis and direct current electric fields.
The present study demonstrates the manipulation of protein particles employing insulator-based dielectrophoresis (iDEP) and direct current (d.c.) electric fields. Fluorescently labeled bovine serum albumin (BSA) protein particles were concentrated inside a microchannel that contained an array of glass cylindrical insulating structures. d.c. electric fields were applied and the dielectrophoretic response of the particles was observed as a function of the suspending medium conductivity (25, 50 and 100 microS/cm) and pH (8 and 9). It was shown that the magnitude of the applied electric field (700-1600 V/cm) and suspending medium properties have a strong effect on the dielectrophoretic response of the protein particles. The results presented here are the first report on protein manipulation employing d.c.-iDEP.
Departamento de Biotecnología e Ingeniería de Alimentos y Centro de Biotecnología, Tecnológico de Monterrey, Campus Monterrey, Avenida Eugenio Garza Sada 2501 Sur, Monterrey, N.L. 64849, Mexico. blapizco@itesm.mx
Blanca H
BH
Lapizco-Encinas
Sandra
S
Ozuna-Chacón
Marco
M
Rito-Palomares
D004553:Electric Conductivity; D004574:Electromagnetic Fields; D004586:Electrophoresis / Q000379:methods*; D006863:Hydrogen-Ion Concentration; D008839:Microelectrodes; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010316:Particle Size; D011506:Proteins / Q000737:chemistry* / Q000302:isolation & purification; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000302:isolation & purification
D011506:Proteins; D012710:Serum Albumin, Bovine