Increased expression of OsPT1, a high-affinity phosphate transporter, enhances phosphate acquisition in rice.
Most high-affinity phosphate transporter genes (OsPTs) in rice were highly induced in roots when phosphate was depleted. OsPT1, however, was highly expressed in primary roots and leaves regardless of external phosphate concentrations. This finding was confirmed histochemically using transgenic rice plants that express the GUS reporter gene under the control of the OsPT1 promoter, which exhibited high GUS activity even in the phosphate sufficient condition. Furthermore, transgenic rice plants overexpressing the OsPT1 gene accumulated almost twice as much phosphate in the shoots as did wild-type plants. As a result, transgenic plants had more tillers than did wild-type plants, which is a typical physiological indicator for phosphate status in rice.
Seo
Hyoun-Mi
HM
Department of Molecular Biotechnology, Dong-A University, Busan 604-714, Korea.
Jung
Yunhui
Y
Song
Songyi
S
Kim
Yunhye
Y
Kwon
Tackmin
T
Kim
Doh-Hoon
DH
Jeung
Soon-Jae
SJ
Yi
Young-Byung
YB
Yi
Gihwan
G
Nam
Min-Hee
MH
Nam
Jaesung
J
D001483:Base Sequence; D015152:Blotting, Northern; D017931:DNA Primers; D017930:Genes, Reporter; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D028061:Phosphate Transport Proteins / Q000235:genetics* / Q000378:metabolism; D010710:Phosphates / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011401:Promoter Regions, Genetic
D017931:DNA Primers; D028061:Phosphate Transport Proteins; D010710:Phosphates