Xenon induces transcription of ADNP in neonatal rat brain.
Xenon and other inhalational agents induce cell and organ protection through different and only partially elucidated molecular mechanisms. Anesthesia induced or pharmacologic preconditioning is a recognized mechanism of cell protection. In this study we explored the gene transcription of activity-dependent neuroprotective protein (ADNP) in neonatal rat brain as consequence to xenon exposure, comparing the noble gas to nitrogen. Seven-day-old Sprague Dawley rats were exposed for 120 min to 75% xenon and 25% oxygen or control condition consisting of 75% nitrogen and 25% oxygen (Air). ADNP was found to be differentially expressed by SSH, validated by Relative Real-Time PCR (RT-PCR) and confirmed by western blot and immunohistochemistry. The differential expression of ADNP in the rat neonatal brain may account for the preconditioning and neuroprotective effects exerted by gas xenon.
Department of Surgery, Division of Anesthesiology and Intensive Care, University of Pisa School of Medicine, Pisa, Italy. davidecattano@hotmail.com
Davide
D
Cattano
Simona
S
Valleggi
Daqing
D
Ma
Olga
O
Kastsiuchenka
Antonio
A
Abramo
Pamela
P
Sun
Andrea O
AO
Cavazzana
Gianfranco
G
Natale
Mervyn
M
Maze
Francesco
F
Giunta
D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D015533:Transcriptional Activation / Q000187:drug effects*; D014978:Xenon / Q000494:pharmacology*
D018685:Anesthetics, Inhalation; D009419:Nerve Tissue Proteins; D014978:Xenon