CO-releasing molecules (CORM-2)-liberated CO attenuates leukocytes infiltration in the renal tissue of thermally injured mice.
OBJECTIVE
To determine whether the CO-releasing molecule -liberated CO attenuates infiltration of leukocytes in the renal tissue of thermally injured mice.


METHODS
Twenty-eight mice were assigned to four groups. Mice in sham group (n=7) were underwent sham thermal injury, whereas mice in burn group (n=7) received 15% total body surface area (TBSA) full-thickness thermal injury. Mice in burn+CORM-2 group (n=7) underwent thermal injury followed by immediate administration of CORM-2 (8mg/kg, i.v.), whereas mice in burn+iCORM-2 group (n=7) underwent thermal injury followed by administration of iCORM-2 (an inactive compound used as negative control). Histological alterations and granulocytes infiltration in kidney were assessed alongised PMN accumulation, activation of NF-kBeta, expressions of ICAM-1 and HO-1 expression in renal tissues.


RESULTS
Treatment of thermally injured mice with CORM-2 significantly attenuated PMN accumulation and prevented activation of NF-kBeta in the kidney. This was accompanied by a decrease of the expression of ICAM-1 and an increase in HO-1 expression. In parallel, burn-induced granulocytes infiltration in renal tissue was markedly decreased by treatment with CORM-2.


CONCLUSIONS
CO delivered by CORM-2 attenuates leukocytes infiltration in the kidney of burned mice by interfering with NF-kBeta activation, protein expression of ICAM-1 and therefore suppressing endothelial cells pro-adhesive phenotype.
Sun
Bingwei
B
Department of Burns, Plastic Surgery, Affiliated Hospital, Jiangsu University, Zhenjiang 212001, Jiangsu Province, PR China. sunbinwe@vip.sina.com
Sun
Zhiwei
Z
Jin
Qin
Q
Chen
Xi
X
D000818:Animals; D002056:Burns / Q000473:pathology / Q000628:therapy*; D002248:Carbon Monoxide / Q000737:chemistry*; D042783:Endothelial Cells / Q000166:cytology; D006098:Granulocytes / Q000166:cytology; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D006358:Hot Temperature; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis; D007668:Kidney / Q000378:metabolism / Q000473:pathology*; D007962:Leukocytes / Q000166:cytology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology; D014945:Wound Healing
D016328:NF-kappa B; D018799:Intercellular Adhesion Molecule-1; D002248:Carbon Monoxide; D051547:Heme Oxygenase-1