Characterization of nuclear localization signals of the prototype foamy virus integrase.
To analyse the potential karyophilic activity of prototype foamy viruses (PFVs), we expressed the PFV integrase (IN) and its mutants as fusion proteins with enhanced green fluorescence protein. The subcellular localization of the fusion proteins was investigated by fluorescence microscopy. The PFV IN was found to be karyophilic and targeted the fusion protein to the nucleus. Mutational analyses demonstrated that the PFV IN contains a potent but non-transferable nuclear localization signal (NLS) in its C-terminal domain and contains five arginine and lysine residues between amino acids 308 and 329 that are critical for its NLS function.
Department of Biotechnology, Chung-Ang University, Ansung, Kyungki 456-756, Republic of Korea.
Dog Gn
DG
An
Department of Biotechnology, Chung-Ang University, Ansung, Kyungki 456-756, Republic of Korea.
Usok
U
Hyun
Department of Biotechnology, Chung-Ang University, Ansung, Kyungki 456-756, Republic of Korea.
Cha-Gyun
CG
Shin
D000818:Animals; D019677:Artificial Gene Fusion; D019556:COS Cells; D002467:Cell Nucleus / Q000737:chemistry; D002522:Chlorocebus aethiops; D003593:Cytoplasm; D004252:DNA Mutational Analysis; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D019426:Integrases / Q000737:chemistry / Q000235:genetics*; D008856:Microscopy, Fluorescence; D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D016092:Spumavirus / Q000201:enzymology / Q000235:genetics*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
D019913:Nuclear Localization Signals; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins; D019426:Integrases