High-density linkage maps and sex-linked markers for the black tiger shrimp (Penaeus monodon).
We report on the construction of sex-specific high-density linkage maps and identification of sex-linked markers for the black tiger shrimp (Penaeus monodon). Overall, we identified 44 male and 43 female linkage groups (2n = 88) from the analysis of 2,306 AFLP markers segregating in three full-sib families, covering 2,378 and 2,362 cM, respectively. Twenty-one putatively homologous linkage groups, including the sex-linkage groups, were identified between the female and male linkage maps. Six sex-linked AFLP marker alleles were inherited from female parents in the three families, suggesting that the P. monodon adopts a WZ-ZZ sex-determining system. Two sex-linked AFLP markers, one of which we converted into an allele-specific assay, confirmed their association with sex in a panel of 52 genetically unrelated animals.
Department of Plant Systems Biology, VIB, B-9052 Ghent, Belgium.
Jan
J
Staelens
Debbie
D
Rombaut
Ilse
I
Vercauteren
Brad
B
Argue
John
J
Benzie
Marnik
M
Vuylsteke
D000483:Alleles; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D004247:DNA / Q000235:genetics; D005260:Female; D005819:Genetic Markers; D008297:Male; D008969:Molecular Sequence Data; D033561:Penaeidae / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid; D012730:Sex Chromosomes / Q000235:genetics*; D019849:Sex Determination Processes
D005819:Genetic Markers; D004247:DNA