Characterization and fermentability of an ethanol soluble high molecular weight coffee fraction.
Brews from differently roasted Arabica coffees were shown to contain 8-12% ethanol soluble substances with molecular masses greater than 2 kDa, possibly contributing to their dietary fiber contents. About 13% of these substances were nondigestible carbohydrates, mainly arabinogalactans. The nondigestible high molecular weight ethanol soluble fraction (HESF) of the medium roasted coffee brew was further characterized and subjected to in vitro fermentation with human fecal bacteria. In addition to carbohydrates, HESF contained proteins/peptides (approximately 20%), but the main fraction was composed of structurally unknown Maillard reaction products. From NMR spectroscopy, we conclude that intact caffeic and ferulic acid derivatives were not incorporated into the melanoidins to a significant extent. Stepwise ultrafiltration and gel filtration indicated a large variation in the molecular weights of HESF constituents. Coffee HESF was shown to be less fermentable by fecal bacteria than soluble coffee fiber isolated by the enzymatic-gravimetric methodology, and because of its lower carbohydrate content, less short-chain fatty acids were produced during the fermentation. Total cell counts, destructive chemical analysis, and NMR spectroscopy indicated that coffee carbohydrates are the preferred substrates for colonic microbiota. However, NMR spectra, absorbances at 405 nm, and nonprotein nitrogen contents showed that noncarbohydrate and nonprotein compounds were also utilized to some extent but the bacterial species involved in this degradation remain to be identified.
Department of Food Chemistry, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Diana
D
Gniechwitz
Nicole
N
Reichardt
Ernst
E
Meiss
John
J
Ralph
Hans
H
Steinhart
Michael
M
Blaut
Mirko
M
Bunzel
D001419:Bacteria / Q000378:metabolism; D002241:Carbohydrates / Q000032:analysis; D003069:Coffee / Q000737:chemistry*; D000431:Ethanol; D005243:Feces / Q000382:microbiology; D005285:Fermentation; D005685:Galactans / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy; D015416:Maillard Reaction; D008970:Molecular Weight; D012995:Solubility
D002241:Carbohydrates; D003069:Coffee; D005685:Galactans; C528200:arabinoglucogalactan; D000431:Ethanol