Organometallic activation of a fluorogen for templated nucleic acid detection.
A nucleic acid detection scheme that employs DNA-mediated delivery of an organomercury activator to unmask a fluorophore is described. The approach relies on adjacent hybridization of two oligonucleotide conjugates containing organomercury and caged rhodamine functionalities. Postsynthetic conjugation of amino-modified DNAs enabled efficient preparation of these probes. Complementary DNA templates yielded fluorescence signals arising from metal-assisted rhodamine uncaging.
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Raphael M
RM
Franzini
Eric T
ET
Kool
D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes; D011905:Genes, ras / Q000235:genetics*; D008969:Molecular Sequence Data; D015394:Molecular Structure; D015345:Oligonucleotide Probes; D009941:Organomercury Compounds / Q000737:chemistry*; D012235:Rhodamines / Q000737:chemistry; D012689:Sequence Homology, Nucleic Acid
D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009941:Organomercury Compounds; D012235:Rhodamines; D004247:DNA