Absence of experimental cross-protection induced by a Trypanosoma cruzi-like strain isolated from bats.
This study evaluated the possibility of inoculation and reinoculation with a trypanosomatid isolated from bats that is morphologically, biologically and molecularly similar to Trypanosoma cruzi, to protect against infection by virulent strains. Non-isogenic mice were divided into 24 groups that received from zero to three inoculations of Trypanosoma cruzi-like strain RM1, in the presence or absence of Freunds adjuvant, and were challenged with the VIC or JG strains of Trypanosoma cruzi. Parasitemia and survival were monitored and animals were sacrificed for histopathological analysis. Animals immunized with Trypanosoma cruzi-like strain RM1 presented decreased parasitemia, independently of the number of inoculations or the presence of adjuvant. In spite of this reduction, these animals did not present any protection against histopathological lesions. Severe eosinophilic infiltrate was observed and was correlated with the number of inoculations of Trypanosoma cruzi-like strain RM1. These findings suggest that prior inoculation with this strain did not protect against infection but, rather, aggravated the tissue inflammatory process.
Departamento de Ciências Biológicas, Universidade Federal do Triângulo Mineiro, Uberaba, MG.
Gabriel Antonio Nogueira
GA
Nascentes
Wendell Sérgio Ferreira
WS
Meira
Eliane
E
Lages-Silva
Luis Eduardo
LE
Ramírez
D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000818:Animals; D014355:Chagas Disease / Q000276:immunology* / Q000517:prevention & control; D002685:Chiroptera / Q000469:parasitology; D003429:Cross Reactions / Q000276:immunology; D004802:Eosinophilia / Q000276:immunology* / Q000469:parasitology; D005620:Freund's Adjuvant / Q000627:therapeutic use; D007106:Immune Sera / Q000008:administration & dosage / Q000276:immunology*; D007116:Immunization, Passive / Q000379:methods*; D051379:Mice; D018512:Parasitemia / Q000276:immunology* / Q000469:parasitology; D014349:Trypanosoma cruzi / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity
D000276:Adjuvants, Immunologic; D007106:Immune Sera; D005620:Freund's Adjuvant