An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase.
Gene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r(2) correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.
Roche Molecular Systems, Inc., Department of Genomics and Oncology, Pleasanton, CA, USA. alexander.kohlmann@roche.com
Alexander
A
Kohlmann
Thomas J
TJ
Kipps
Laura Z
LZ
Rassenti
James R
JR
Downing
Sheila A
SA
Shurtleff
Ken I
KI
Mills
Amanda F
AF
Gilkes
Wolf-Karsten
WK
Hofmann
Giuseppe
G
Basso
Marta Campo
MC
Dell'orto
Robin
R
Foà
Sabina
S
Chiaretti
John
J
De Vos
Sonja
S
Rauhut
Peter R
PR
Papenhausen
Jesus M
JM
Hernández
Eva
E
Lumbreras
Allen E
AE
Yeoh
Evelyn S
ES
Koay
Rachel
R
Li
Wei-Min
WM
Liu
Paul M
PM
Williams
Lothar
L
Wieczorek
Torsten
T
Haferlach
D000230:Adenocarcinoma / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics; D002277:Carcinoma / Q000235:genetics; D005060:Europe; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards*; D006801:Humans; D007938:Leukemia / Q000175:diagnosis / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards*; D010865:Pilot Projects; D012313:RNA; D012015:Reference Standards; D015203:Reproducibility of Results; D012846:Singapore; D014481:United States
D012313:RNA