Examination of transcript amounts and activity of protein kinase CK2 in muscle lysates of different types of human muscle pathologies.
Motoneurons release the heparansulfate proteoglycan agrin and thereby activate the muscle-specific receptor tyrosine kinase (MuSK), which is the main organizer of subsynaptic specializations at the neuromuscular junction. Recently, we showed that (1) the protein kinase CK2 interacts with the intracellular region of MuSK; (2) the CK2 protein is enriched and co-localized with MuSK at postsynaptic specializations; (3) CK2-mediated phosphorylation of serine residues within a specific MuSK epitope, named the kinase insert, regulates acetylcholine receptor (AChR) clustering; (4) muscle-specific CK2beta knockout mice develop a myasthenic phenotype due to impaired muscle endplate structure and function (see Genes Dev 20(13):1800-1816, 2006). Here, we investigated for the first time if CK2 is modulated in biopsies from human patients. To this end, we measured transcript amounts of the subunits CK2alpha and CK2beta and determined holoenzyme CK2 activity in 34 muscle biopsies of human patients with different muscle pathologies.
Neurologische Klinik, Universität Erlangen-Nürnberg, Schwabachanlage 6, 91054, Erlangen, Germany.
Dieter
D
Heuss
Janine
J
Klascinski
Steffen W
SW
Schubert
Tehmur
T
Moriabadi
Hanns
H
Lochmüller
Said
S
Hashemolhosseini
D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D001706:Biopsy; D016022:Case-Control Studies; D047390:Casein Kinase II / Q000235:genetics* / Q000378:metabolism*; D002648:Child; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006801:Humans; D007223:Infant; D008297:Male; D051379:Mice; D008875:Middle Aged; D009132:Muscles / Q000201:enzymology* / Q000473:pathology*; D009135:Muscular Diseases / Q000201:enzymology* / Q000235:genetics*; D021122:Protein Subunits / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014020:Tissue Extracts / Q000378:metabolism*
D021122:Protein Subunits; D012333:RNA, Messenger; D014020:Tissue Extracts; D047390:Casein Kinase II