Towards a universally adaptable method for quantitative extraction of high-purity nucleic acids from soil.
A universally adaptable protocol for quantitative extraction of high-purity nucleic acids from soil is presented. A major problem regarding the extraction of nucleic acids from soil is the presence of humic substances, which interfere with the extraction process itself and in subsequent analytical manipulations. By the approach described here, the humic compounds are precipitated prior to cell lysis with Al(2)(SO(4))(3), and thus eliminated prior to the nucleic acid extraction. The protocol allows for removing of a considerable content and range of humic acids and should therefore be applicable for a wide spectrum of soil types. Accordingly, reproducible results in analyses of different soil types are made possible, inclusively for quantitative comparisons.
Persoh
Derek
D
Lehrstuhl für Pflanzensystematik, Universität Bayreuth, Universitätsstrasse 30 NW I, D-95440 Bayreuth, Germany. derek.persoh@uni-bayreuth.de
Theuerl
Susanne
S
Buscot
François
F
Rambold
Gerhard
G
D005821:Genetic Techniques; D006812:Humic Substances / Q000032:analysis; D009696:Nucleic Acids / Q000032:analysis* / Q000235:genetics / Q000302:isolation & purification*; D012987:Soil / Q000032:analysis*; D012988:Soil Microbiology
D006812:Humic Substances; D009696:Nucleic Acids; D012987:Soil