Role of glutathione metabolism status in the definition of some cellular parameters and oxidative stress tolerance of Saccharomyces cerevisiae cells growing as biofilms.
The resistance of Saccharomyces cerevisiae to oxidative stress (H(2)O(2) and Cd(2+)) was compared in biofilms and planktonic cells, with the help of yeast mutants deleted of genes related to glutathione metabolism and oxidative stress. Biofilm-forming cells were found predominantly in the G1 stage of the cell cycle. This might explain their higher tolerance to oxidative stress and the young replicative age of these cells in an old culture. The reduced glutathione status of S. cerevisiae was affected by the growth phase and apparently plays an important role in oxidative stress tolerance in cells growing as a biofilm.
Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, Faculté de Pharmacie, CNRS Nancy-University, Nancy Cedex, France.
Grégoire
G
Gales
Michel
M
Penninckx
Jean-Claude
JC
Block
Pierre
P
Leroy
D000935:Antifungal Agents / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects*; D002104:Cadmium / Q000494:pharmacology; D005656:Fungal Proteins / Q000235:genetics; D017353:Gene Deletion; D005978:Glutathione / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D016877:Oxidants / Q000494:pharmacology*; D018384:Oxidative Stress; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000502:physiology*
D000935:Antifungal Agents; D005656:Fungal Proteins; D016877:Oxidants; D002104:Cadmium; D006861:Hydrogen Peroxide; D005978:Glutathione