A rapid and sensitive chemiluminescent immunoassay of total thyroxin with DMAE x NHS-Labeled.
A novel chemiluminescence immunoassay (CLIA) of total thyroxin (TT4) with the acridinium ester of DMAE x NHS [2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-10-methyl-acridinium-9-carboxylate methosulfate] labeled has been developed. In our method, microwells were coated with anti-T4 monoclonal antibody (McAb) and DMAE x NHS synthesized by our laboratory was conjugated with streptavidin (SA). T4-BSA was conjugated with biotin-N-hydroxysuccinimide (B x NHS), T4-BSA- B x NHS and the T4 in the standard or sample competitively react with anti-T4 McAb. Streptavidin-biotin separation and enhanced techniques were applied in this assay. The presented approach shows many excellent characteristics, particularly, rapid assay process and high detection sensitivity.
Yin
Dongguang
D
Shanghai Applied Radiation Institute, College of Environmental and Chemical Engineering, Shanghai University, Jiadin, PR China.
Cui
Daxiang
D
Gao
Feng
F
He
Rong
R
He
Youfeng
Y
Liu
Yibing
Y
Shen
Decun
D
Wu
Minghong
M
D000166:Acridines; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D012680:Sensitivity and Specificity; D013463:Sulfuric Acid Esters; D013959:Thyroid Diseases / Q000175:diagnosis; D013974:Thyroxine / Q000097:blood*
C530833:2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-10-methyl-acridinium-9-carboxylate methosulfate; D000166:Acridines; D013463:Sulfuric Acid Esters; D013974:Thyroxine