Technique for the early detection of drug-resistant HBV DNA during antiviral therapy.
Early detection of antiviral resistant mutants is of clinical importance in patients receiving antiviral treatment. The ideal assay is sensitive, specific, accurate, reproducible and easy to perform, attains a high throughput and is able to detect mixed and novel mutations. Advantages and disadvantages of sequencing, line probe assay, DNA chip technology, peptide nucleic acid cramping, fluorescent biprobe hybridization and a new technique based on matrix-assisted laser desorption/ionization time-of-flight mass-spectrometric analysis will be discussed.
Department of Internal Medicine, Division of Gastroenterology and Hepatology, Korea University College of Medicine, Korea University Guro Hospital, Seoul, Republic of Korea. je_yeon@hotmail.com
Jong Eun
JE
Yeon
D000998:Antiviral Agents / Q000494:pharmacology*; D004279:DNA, Viral / Q000235:genetics; D024882:Drug Resistance, Viral / Q000235:genetics; D005470:Fluorometry; D006509:Hepatitis B / Q000188:drug therapy* / Q000821:virology; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D009154:Mutation; D009693:Nucleic Acid Hybridization; D020411:Oligonucleotide Array Sequence Analysis; D020135:Peptide Nucleic Acids / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000998:Antiviral Agents; D004279:DNA, Viral; D020135:Peptide Nucleic Acids