Relative expression of genes involved in the resistance/sensitivity of Enterococcus faecalis JH2-2 to recombinant divercin RV41.
The relative expression of mptABCD operon, glpQ, pde, rpoN, mptR and gap-1 was studied by reverse transcription combined with the real time polymerase chain reaction to understand the role of each gene in resistance/sensitivity of Enterococcus faecalis to class IIa bacteriocins such as recombinant divercin V41 (DvnRV41). Comparative critical threshold methods in presence or absence of DvnRV41 were then used to determine the level of expression of each gene cited above. In the presence of DvnRV41, the rpoN and glpQ genes were down-regulated, mptR, mptC, gap-1 and pde genes were up-regulated, whilst expression of mptB and mptD genes remained unmodified.
Laboratoire de Microbiologie, ENITIAA, Rue de la Géraudière, BP 82225, 44322 Nantes Cedex 3, France.
Ségolène
S
Calvez
H
H
Prevost
Djamel
D
Drider
D001430:Bacteriocins / Q000235:genetics*; D001483:Base Sequence; D017931:DNA Primers; D013293:Enterococcus faecalis / Q000235:genetics*; D015870:Gene Expression; D005798:Genes, Bacterial; D012329:RNA, Bacterial / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001430:Bacteriocins; D017931:DNA Primers; D012329:RNA, Bacterial; D011994:Recombinant Proteins; C116372:divercin V41