Determination of triterpene glycosides in sea cucumber (Stichopus japonicus) and its related products by high-performance liquid chromatography.
A creative and sensitive method has been developed for the determination of triterpene glycosides concentrations in sea cucumber ( Stichopus japonicus) and related products by using d-quinovose (6-deoxyglucose) as the measurement standard by reverse-phase high-performance liquid chromatography (HPLC) and variable-wavelength detection. d-quinovose, which is a unique monosaccharide in holostane triterpene glycosides, was liberated by acid hydrolysis and precolumn derivatized by 1-phenyl-3-methyl-5-pyrazolone (PMP). PMP-quinovose was analyzed by HPLC with 22% acetonitrile in 0.05 M KH2PO4 aquatic solution (pH 5.2) as mobile phase. The calibration curves of d-quinovose were linear within the range of 6.56-164 mg/L (r(2) > 0.995). The contents of triterpene glycosides in various S. japonicus products were determined after appropriate pretreatment methods. The concentration of triterpene glycosides was calculated by the formula C = C(qui) x alpha (alpha = 8.5). The result showed that this method was a simple, rapid, and stable method for the determination of triterpene glycosides in S. japonicus products.
Dong
Ping
P
College of Food Science and Technology, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, People's Republic of China.
Xue
Chang-Hu
CH
Yu
Lin-Fang
LF
Xu
Jie
J
Chen
Shi-Guo
SG
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000032:analysis*; D012616:Sea Cucumbers / Q000737:chemistry* / Q000378:metabolism; D017747:Seafood / Q000032:analysis; D013048:Specimen Handling; D014315:Triterpenes / Q000032:analysis*
D006027:Glycosides; D014315:Triterpenes