Quantitative measurement of oxygen consumption in insect cell culture infected with polyhedrosis virus.
A simple and reproducible quantitative method for measuring dissolved oxygen (DO) in uninfected and baculovirus infected cells in culture is described. To establish this method, an industrial DO measuring system for fermentation was employed. During this process the physical characteristics of the cell culture vessel were taken into account permitting a direct readout of DO in microliters per vessel. During these studies, it was experimentally documented that insect cells, particularly baculovirus infected cells, in culture from 1 to 14 days utilize an appreciable level of DO.
Microbiology and Infectious Diseases, Southwest oundation for Research and Education, San Antonio, Texas 78284.
S A
SA
Weiss
T
T
Orr
G C
GC
Smith
S S
SS
Kalter
J L
JL
Vaughn
E M
EM
Dougherty