Phage types and pulsed-field gel electrophoresis patterns of Salmonella enterica serovar Enteritidis isolated from humans and chickens.
We analyzed 66 Salmonella Enteritidis isolates in 2002. Thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. A total of ten phage types (PT) were identified in the human and chicken isolates. PT1 and PT21 were the predominant PTs in both the human (20% and 13%) and chicken (17% and 47%) isolates. Twelve pulsotypes were generated by PFGE and divided into two major groups. Most of the PFGE types were categorized into cluster group 1. Eighteen chicken isolates in cluster group 1 showed high-level genetic association (>95%) with 22 other human isolates. Additionally, six chicken isolates from cluster group 2 showed fairly high-level genetic association (>95%) with the other seven human isolates. The highest levels of genetic association in humans and chickens were seen with A5-PT21 (11 isolates), A2-PT1 (7 isolates), and B1-PT4 (6 isolates). The Pulsed-Field Gel Electrophoresis (PFGE) and phage typing provided conclusive evidence that human Salmonella infections are attributable to the consumption of contaminated chicken.
Kim
Sung Hun
SH
Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul 122-701, Republic of Korea.
Kim
Shukho
S
Chun
Sung Guen
SG
Park
Mi-Sun
MS
Park
Jeong Hyun
JH
Lee
Bok-Kwon
BK
D000818:Animals; D001434:Bacteriophage Typing; D002645:Chickens; D004269:DNA, Bacterial / Q000032:analysis; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D010802:Phylogeny; D011201:Poultry Diseases / Q000382:microbiology*; D012480:Salmonella Infections / Q000382:microbiology* / Q000821:virology; D012483:Salmonella Phages / Q000145:classification* / Q000302:isolation & purification*; D019779:Salmonella enterica / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000821:virology
D004269:DNA, Bacterial