Development of an automated on-line pepsin digestion-liquid chromatography-tandem mass spectrometry configuration for the rapid analysis of protein adducts of chemical warfare agents.
Rapid monitoring and retrospective verification are key issues in protection against and non-proliferation of chemical warfare agents (CWA). Such monitoring and verification are adequately accomplished by the analysis of persistent protein adducts of these agents. Liquid chromatography-mass spectrometry (LC-MS) is the tool of choice in the analysis of such protein adducts, but the overall experimental procedure is quite elaborate. Therefore, an automated on-line pepsin digestion-LC-MS configuration has been developed for the rapid determination of CWA protein adducts. The utility of this configuration is demonstrated by the analysis of specific adducts of sarin and sulfur mustard to human butyryl cholinesterase and human serum albumin, respectively.
Department of Analytical Chemistry and Applied Spectroscopy, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands.
Jeroen
J
Carol-Visser
Marcel
M
van der Schans
Alex
A
Fidder
Albert G
AG
Hulst
Ben L M
BL
van Baar
Hubertus
H
Irth
Daan
D
Noort
D002619:Chemical Warfare Agents / Q000737:chemistry*; D002802:Cholinesterases / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D009151:Mustard Gas / Q000737:chemistry; D009862:Online Systems / Q000295:instrumentation*; D010434:Pepsin A / Q000378:metabolism*; D012524:Sarin / Q000737:chemistry; D012709:Serum Albumin / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
D002619:Chemical Warfare Agents; D012709:Serum Albumin; D012524:Sarin; D002802:Cholinesterases; D010434:Pepsin A; D009151:Mustard Gas