Synthesis of fluorogenic polymers for visualizing cellular internalization.
The binding of a polymeric ligand to a cell surface receptor can promote its internalization. Methods to track and visualize multivalent ligands within a cell can give rise to new therapeutic strategies and illuminate signaling processes. We have used the features of the ring-opening metathesis polymerization (ROMP) to develop a general strategy for synthesizing multivalent ligands equipped with a latent fluorophore. The utility of ligands of this type is highlighted by visualizing multivalent antigen internalization in live B cells.
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Shane L
SL
Mangold
Rachael T
RT
Carpenter
Laura L
LL
Kiessling
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology / Q000502:physiology; D043182:Carboxylesterase / Q000378:metabolism; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D008099:Liver / Q000201:enzymology; D008856:Microscopy, Fluorescence; D015394:Molecular Structure; D011108:Polymers / Q000138:chemical synthesis*; D011956:Receptors, Cell Surface / Q000187:drug effects*; D013552:Swine
D005456:Fluorescent Dyes; D011108:Polymers; D011956:Receptors, Cell Surface; D043182:Carboxylesterase