Molecular assessment of genetic diversity in mung bean germplasm.
RAPD profiles were used to identify the extent of diversity among 54 accessions of mung bean that included both improved and local land races. Out of the 40 primers screened, seven primers generated 174 amplification products with an average of 24.85 bands per primer. The RAPD profiles were analysed for Jaccard's similarity coefficients that was found to be in the range from 0 to 0.48, indicating the presence of wide range of genetic diversity at molecular level. Cluster analysis was carried out based on distances (1-similarity coefficient) using neighbour-joining method in Free Tree package. The dendrogram resolved all the accessions into two major clusters, I (with 11 accessions) and II (with 43 accessions). However, the cluster was further divided into four subclusters (II A with six, II B with nine, II C with 15 and II D with 13 accessions). The distribution of the accessions in different clusters and subclusters appears to be related to their performance in field conditions for 10 morphological traits that were scored. This study indicated that the RAPD profiles provide an easy and simple technique for preliminary genetic diversity assessment of mung bean accessions that may reflect morphological trait differences among them.
Lavanya
G Roopa
GR
Department of Genetics and Plant Breeding, Allahabad Agricultural Institute Deemed University, Allahabad 211 007, India. lavanya.roopa@gmail.com
Srivastava
Jyoti
J
Ranade
Shirish A
SA
D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D007887:Fabaceae / Q000235:genetics*; D014644:Genetic Variation; D019105:Random Amplified Polymorphic DNA Technique
D017931:DNA Primers; D018744:DNA, Plant