[Experimental study on thermochemotherapy combined with verapamil for reversing the multidrug resistance responsible to breast cancer cell].
OBJECTIVE
To reverse the multidrug resistance of MCF-7/ADM cell line by thermochemotherapy combined with verapamil (VRP).


METHODS
The drug resistance cells MCF-7/ADM were treated with 3 microg/mL Adriamycin and different concentration VRP (0-5 micromol/L), and then cultured with 37-43 degrees C for 30 minutes. Confocal microscopy was used to demonstrate the fluorescence adriamycin in cells, and the cell apoptosis rate was analyzed with flow cytometry.


RESULTS
With the increases of thermochemical temperature and VRP concentration, the cell adriamycin fluorescence intensity and apoptosis rate of MCF-7/ADM were raised. Thermochemotherapy showed marked synergistic effect with VRP in MCF-7/ADM.


CONCLUSIONS
It is suggested that thermochemotherapy combined with Verapamil could be used as a way to overcome the multidrug resistance.
Cancer Center, West China Hostipal, Sichuan University, Chengdu 610041, China.
Xi
X
Yan
Mei
M
Hou
Sheng-Fu
SF
Li
Yuan-Yuan
YY
Liu
D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000473:pathology / Q000628:therapy; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D004317:Doxorubicin / Q000494:pharmacology*; D018432:Drug Resistance, Multiple / Q000187:drug effects*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004357:Drug Synergism; D005260:Female; D006358:Hot Temperature; D006801:Humans; D018613:Microscopy, Confocal; D014700:Verapamil / Q000494:pharmacology*
D004317:Doxorubicin; D014700:Verapamil