Regulation and role of Arabidopsis CUL4-DDB1A-DDB2 in maintaining genome integrity upon UV stress.
Plants use the energy in sunlight for photosynthesis, but as a consequence are exposed to the toxic effect of UV radiation especially on DNA. The UV-induced lesions on DNA affect both transcription and replication and can also have mutagenic consequences. Here we investigated the regulation and the function of the recently described CUL4-DDB1-DDB2 E3 ligase in the maintenance of genome integrity upon UV-stress using the model plant Arabidopsis. Physiological, biochemical, and genetic evidences indicate that this protein complex is involved in global genome repair (GGR) of UV-induced DNA lesions. Moreover, we provide evidences for crosstalks between GGR, the plant-specific photo reactivation pathway and the RAD1-RAD10 endonucleases upon UV exposure. Finally, we report that DDB2 degradation upon UV stress depends not only on CUL4, but also on the checkpoint protein kinase Ataxia telangiectasia and Rad3-related (ATR). Interestingly, we found that DDB1A shuttles from the cytoplasm to the nucleus in an ATR-dependent manner, highlighting an upstream level of control and a novel mechanism of regulation of this E3 ligase.
Molinier
Jean
J
Institut de Biologie Moléculaire des Plantes du CNRS (UPR2357), conventionné avec l'Université Louis Pasteur, Strasbourg, France.
Lechner
Esther
E
Dumbliauskas
Eva
E
Genschik
Pascal
P
D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D064007:Ataxia Telangiectasia Mutated Proteins; D002135:Calcium-Binding Proteins / Q000235:genetics / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000378:metabolism; D044842:Cullin Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D004249:DNA Damage / Q000528:radiation effects; D004260:DNA Repair / Q000528:radiation effects; D004268:DNA-Binding Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000528:radiation effects; D018745:Genome, Plant / Q000528:radiation effects*; D016254:Mutagenesis, Insertional; D030821:Plants, Genetically Modified / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism; D021381:Protein Transport; D011836:Radiation Tolerance; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014466:Ultraviolet Rays
D029681:Arabidopsis Proteins; C487571:CEN2 protein, Arabidopsis; C514806:CULLIN4 protein, Arabidopsis; D002135:Calcium-Binding Proteins; D018797:Cell Cycle Proteins; D044842:Cullin Proteins; C517426:DDB1a protein, Arabidopsis; C530204:DDB2 protein, Arabidopsis; D004268:DNA-Binding Proteins; C514695:PHR1 protein, Arabidopsis; D014157:Transcription Factors; C576892:ATR protein, Arabidopsis; D064007:Ataxia Telangiectasia Mutated Proteins; D017346:Protein Serine-Threonine Kinases