Development of a serotyping ELISA system for Thailand virus infection.
To distinguish Thailand virus infection from infections with other hantaviruses, we established an ELISA serotyping system using a truncated nucleocapsid protein of Thailand virus lacking 49 amino acids at the N-terminus. In evaluations using patient and rodent sera, Thailand virus infection was readily distinguished from Hantaan and Seoul virus infections. Therefore, this ELISA system is an effective alternative to neutralization tests.
Nakamura
Ichiro
I
Research Center for Zoonosis Control, Hokkaido University, Kita-ku, Sapporo, Hokkaido, Japan.
Yoshimatsu
Kumiko
K
Lee
Byoung-Hee
BH
Okumura
Megumi
M
Taruishi
Midori
M
Araki
Koichi
K
Kariwa
Hiroaki
H
Takashima
Ikuo
I
Arikawa
Jiro
J
D000914:Antibodies, Viral / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D006476:Orthohantavirus / Q000145:classification* / Q000302:isolation & purification; D018778:Hantavirus Infections / Q000175:diagnosis* / Q000276:immunology; D006801:Humans; D007106:Immune Sera / Q000276:immunology; D009500:Neutralization Tests; D019590:Nucleocapsid Proteins / Q000276:immunology; D012703:Serotyping; D013785:Thailand
D000914:Antibodies, Viral; D007106:Immune Sera; D019590:Nucleocapsid Proteins