Characterization of a novel portal protein from deep-sea thermophilic bacteriophage GVE2.
Portal proteins, located asymmetrically at one of the twelve vertices of the capsid, play very important roles in viral DNA packaging. Compared with the well-studied portal proteins of bacteriophages infecting mesophilic bacteria, portal proteins of thermophilic bacteriophages from deep sea have not been characterized. In this investigation, a novel portal protein was identified from a deep-sea thermophilic bacteriophage GVE2 for the first time. The GVE2 portal protein (designated as VP411 protein) shared low similarity to known portal proteins from other species, but they showed high similarities in the predicted secondary structures, suggesting that they had the same function in viral DNA packaging. The Northern blot and Western bolt results demonstrated that the vp411 gene was expressed in the late stage of GVE2 infection, implying that it might be a viral late gene. As revealed by immuno-electron microscopy, the gold particles were observed in the junction between the phage head and the phage tail when the anti-VP411 IgG was used as the primary antibody, indicating that it had the location in the virion expected of a portal protein.
Wang
Yiqian
Y
School of Life Sciences, Xiamen University, Xiamen 361005, The People's Republic of China.
Zhang
Xiaobo
X
D000595:Amino Acid Sequence; D001435:Bacteriophages / Q000737:chemistry / Q000235:genetics* / Q000648:ultrastructure; D036022:Capsid Proteins / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D015870:Gene Expression; D006358:Hot Temperature; D008969:Molecular Sequence Data; D009792:Oceans and Seas; D017386:Sequence Homology, Amino Acid; D014771:Virion / Q000737:chemistry / Q000648:ultrastructure
D036022:Capsid Proteins