Heterologous expression of a position 2-substituted (1-->3)-beta-D-glucan in Lactococcus lactis.
Exopolysaccharides play an important role in the rheology and texture of fermented foods, and among these beta-glucans have immunomodulating properties. We show that the overproduction of the Pediococcus parvulus GTF glycosyltransferase in an uncapsulated Lactococcus lactis strain results in synthesis and secretion (300 mg liter(-1)) of a position 2-substituted (1-->3)-beta-D-glucan that has potential use as a food additive.
Werning
María Laura
ML
Departamento de Ciencia de Proteínas, Centro de Investigaciones Biológicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.
Corrales
María Angeles
MA
Prieto
Alicia
A
de Palencia
Pilar Fernández
PF
Navas
Jesús
J
López
Paloma
P
D005516:Food Microbiology; D015870:Gene Expression; D005822:Genetic Vectors; D016695:Glycosyltransferases / Q000378:metabolism*; D013294:Lactococcus lactis / Q000201:enzymology / Q000378:metabolism*; D010376:Pediococcus / Q000201:enzymology* / Q000235:genetics; D010957:Plasmids; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis*; D047071:beta-Glucans / Q000302:isolation & purification / Q000378:metabolism*
D011135:Polysaccharides, Bacterial; D047071:beta-Glucans; D016695:Glycosyltransferases