Determination of triterpene glycosides in sea cucumber (Stichopus japonicus) and its related products by high-performance liquid chromatography.
A creative and sensitive method has been developed for the determination of triterpene glycosides concentrations in sea cucumber ( Stichopus japonicus) and related products by using d-quinovose (6-deoxyglucose) as the measurement standard by reverse-phase high-performance liquid chromatography (HPLC) and variable-wavelength detection. d-quinovose, which is a unique monosaccharide in holostane triterpene glycosides, was liberated by acid hydrolysis and precolumn derivatized by 1-phenyl-3-methyl-5-pyrazolone (PMP). PMP-quinovose was analyzed by HPLC with 22% acetonitrile in 0.05 M KH2PO4 aquatic solution (pH 5.2) as mobile phase. The calibration curves of d-quinovose were linear within the range of 6.56-164 mg/L (r(2) > 0.995). The contents of triterpene glycosides in various S. japonicus products were determined after appropriate pretreatment methods. The concentration of triterpene glycosides was calculated by the formula C = C(qui) x alpha (alpha = 8.5). The result showed that this method was a simple, rapid, and stable method for the determination of triterpene glycosides in S. japonicus products.
College of Food Science and Technology, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, People's Republic of China.
Ping
P
Dong
Chang-Hu
CH
Xue
Lin-Fang
LF
Yu
Jie
J
Xu
Shi-Guo
SG
Chen
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D006027:Glycosides / Q000032:analysis*; D012616:Sea Cucumbers / Q000737:chemistry* / Q000378:metabolism; D017747:Seafood / Q000032:analysis; D013048:Specimen Handling; D014315:Triterpenes / Q000032:analysis*
D006027:Glycosides; D014315:Triterpenes