Mechanistic analysis of complex enzyme deactivations: influence of various parameters on series-type inactivations.
A series-type enzyme deactivation model is used to model and to quantitate some more complex enzyme deactivations. The influence of temperature, pH, immobilization, chemical modifier (inhibitor or protector), substrate, and metal ion on the inactivation kinetics and on the parameter values is examined. In some cases the influence of two parameters on enzyme inactivations is presented. This provides further physical insights into enzyme inactivation and stabilization processes.
Sadana
A
A
Chemical Engineering Department, University of Mississippi, University, Mississippi 38677, USA.
Henley
J P
JP