Localization and expression of serine racemase in Arabidopsis thaliana.
Arabidopsis plants transformed by promoter of A. thaliana serine racemase fused with beta-glucuronidase (GUS) reporter gene showed strong GUS staining in elongating and developing cells such as tip regions of primary and lateral roots, developing leaves, and shoot meristems. RT-PCR and digital northern hybridization showed that expression of the serine racemase gene was not induced by L- and D-serine, light irradiation, biotic and abiotic stresses.
Group of Cytomolecular Biochemistry, Research Institute for Bioresources, Okayama University, 2-20-1 Chuo, Kurashiki, Okayama 710-0046, Japan. manabus@rib.okayama-u.ac.jp
Manabu
M
Sugimoto
Wataru
W
Sakamoto
Yoshiyuki
Y
Fujitani
D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005966:Glucuronidase / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics; D019998:Racemases and Epimerases / Q000235:genetics / Q000378:metabolism*; D000099300:Serine Racemase
D029681:Arabidopsis Proteins; D005966:Glucuronidase; D019998:Racemases and Epimerases; D000099300:Serine Racemase