Nanometer arrays of functional light harvesting antenna complexes by nanoimprint lithography and host-guest interactions.
We show an approach based on a combination of site-directed mutagenesis, NIL and multivalent host-guest interactions for the realization of engineered ordered functional arrays of purified components of the photosynthetic system, the membrane-bound LH2 complex. In addition to micrometer-scale patterned structures, we demonstrated the use of nanometer-scale hard NIL stamps to generate functional protein arrays approaching molecular dimensions.
Biophysical Engineering Group, MESA+ Institute for Nanotechnology, University of Twente P.O. Box 217, 7500 AE Enschede, The Netherlands.
Maryana
M
Escalante
Yiping
Y
Zhao
Manon J W
MJ
Ludden
Rolf
R
Vermeij
John D
JD
Olsen
Erwin
E
Berenschot
C Neil
CN
Hunter
Jurriaan
J
Huskens
Vinod
V
Subramaniam
Cees
C
Otto
D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics; D005960:Glucosides / Q000737:chemistry; D045342:Light-Harvesting Protein Complexes / Q000737:chemistry* / Q000235:genetics; D018625:Microscopy, Atomic Force; D016297:Mutagenesis, Site-Directed; D036103:Nanotechnology / Q000379:methods*; D012242:Rhodobacter sphaeroides / Q000737:chemistry; D013050:Spectrometry, Fluorescence; D047392:beta-Cyclodextrins / Q000737:chemistry
C081521:B800-850 light-harvesting complex, bacteria; D001426:Bacterial Proteins; D005960:Glucosides; D045342:Light-Harvesting Protein Complexes; D047392:beta-Cyclodextrins; C040358:dodecyl maltoside; C031215:betadex