Low positive predictive value of a nucleic acid amplification test for nongenital Neisseria gonorrhoeae infection in homosexual men.
The diagnosis of nongenital Neisseria gonorrhoeae infection by the Becton Dickinson ProbeTec ET Chlamydia trachomatis and N. gonorrhoeae Amplified DNA assay had low positive predictive value among an Australian community-based sample of homosexual men. Only 30.4% of oropharyngeal samples and 73.7% of anorectal samples were positive for N. gonorrhoeae by the porA assay. The accuracy of nucleic acid amplification tests in this context is compromised without supplemental testing.
Molecular Diagnostic Medicine Laboratory, SydPath, St. Vincent's Hospital, Darlinghurst, NSW. lmcnally@stvincents.com.au
Leon P
LP
McNally
David J
DJ
Templeton
Fengyi
F
Jin
Andrew E
AE
Grulich
Basil
B
Donovan
David M
DM
Whiley
Philip H
PH
Cunningham
D001003:Anal Canal / Q000382:microbiology*; D015331:Cohort Studies; D004269:DNA, Bacterial / Q000032:analysis; D005189:False Positive Reactions; D006069:Gonorrhea / Q000175:diagnosis*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D009960:Oropharynx / Q000382:microbiology*; D018272:Porins / Q000235:genetics; D011237:Predictive Value of Tests
D004269:DNA, Bacterial; D018272:Porins; C057699:porin protein, Neisseria