Foam separation of DNA and proteins from solutions.
Foam separation on BSA-DNA (bovine serum albumine/deoxyribonucleic acid) and Lysozyme-DNA systems is performed. The separation of the total protein from DNA is evaluated for dissociated chromatin solution. Foam separation for the same systems is done also by a new method of creating a pressure gradient in the Plateau-Gibbs borders in the foam and obtaining a "dry" foam. It is shown that the effectiveness of the foam separation can be improved significantly by the application of the latter method. Some factors (pH, initial concentration of the solution, expansion factor of the foam) influencing the separation of proteins from DNA in the foam and in the residual solution are studied as well.
Lalchev
Z
Z
Department of Biochemistry, University of Sofia, 8 Dragan Tzankov str., 1421 Sofia, Bulgaria.
Dimitrova
L
L
Tzvetkova
P
P
Exerowa
D
D