Studies on the protective effect of dietary fish oil on gentamicin-induced nephrotoxicity and oxidative damage in rat kidney.
Gentamicin (GM)-induced nephrotoxicity limits its long-term clinical use. Several agents/strategies were attempted to prevent GM nephrotoxicity but were not found suitable for clinical practice. Dietary fish oil (FO) retard the progression of certain types of cancers, cardiovascular and renal disorders. We aimed to evaluate protective effect of FO on GM-induced renal proximal tubular damage. The rats were pre-fed experimental diets for 10 days and then received GM (80 mg/kg body weight/day) treatment for 10 days while still on diet. Serum/urine parameters, enzymes of carbohydrate metabolism, brush border membrane (BBM), oxidative stress and phosphate transport in rat kidney were analyzed. GM nephrotoxicity was recorded by increased serum creatinine and blood urea nitrogen. GM increased the activities of lactate and glucose-6-phosphate dehydrogenases whereas decreased malate, isocitrate dehydrogenases; glucose-6 and fructose-1,6-bisphosphatases; superoxide dismutase, catalase, glutathione peroxidase and BBM enzymes. In contrast, FO alone increased enzyme activities of carbohydrate metabolism, BBM and oxidative stress. FO feeding to GM treated rats markedly enhanced resistance to GM elicited deleterious effects and prevented GM-induced decrease in 32Pi uptake across BBM. Dietary FO supplementation ameliorated GM-induced specific metabolic alterations and oxidative damage due to its intrinsic biochemical/antioxidant properties.
Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, U.P., India.
Shubha
S
Priyamvada
Medha
M
Priyadarshini
N A
NA
Arivarasu
Neelam
N
Farooq
Sheeba
S
Khan
Sara A
SA
Khan
Md Wasim
MW
Khan
A N K
AN
Yusufi
D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001693:Biological Transport, Active / Q000187:drug effects; D001786:Blood Glucose / Q000378:metabolism; D001806:Blood Urea Nitrogen; D050260:Carbohydrate Metabolism / Q000187:drug effects; D003314:Corn Oil / Q000008:administration & dosage / Q000494:pharmacology / Q000627:therapeutic use; D003404:Creatinine / Q000097:blood; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D004798:Enzymes / Q000378:metabolism; D005395:Fish Oils / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005839:Gentamicins / Q000633:toxicity; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D007672:Kidney Cortex / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D007674:Kidney Diseases / Q000139:chemically induced / Q000378:metabolism / Q000517:prevention & control*; D007679:Kidney Medulla / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008055:Lipids / Q000097:blood; D008297:Male; D008871:Microvilli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008954:Models, Biological; D018384:Oxidative Stress / Q000187:drug effects; D010710:Phosphates / Q000097:blood / Q000378:metabolism / Q000652:urine; D051381:Rats; D017208:Rats, Wistar; D014555:Urination Disorders / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control
D000975:Antioxidants; D001786:Blood Glucose; D004042:Dietary Fats, Unsaturated; D004798:Enzymes; D005395:Fish Oils; D005839:Gentamicins; D008055:Lipids; D010710:Phosphates; D003314:Corn Oil; D003404:Creatinine