[Alteration of retinoid X receptor alpha mRNA level after acitretin and/or narrow-band UVB treatment in normal human keratinocytes].
OBJECTIVE
To investigate the alteration of retinoid X receptor alpha (RXRalpha) mRNA level in normal human keratinocytes after acitretin and/or NB-UVB irradiation treatment.


METHODS
After a 12-hour incubation with 10(-7)-10(-6) mol/L acitretin and/or following 50-100 mJ/cm2 NB-UVB irradiation in normal human keratinocytes, RXRalpha mRNA expression was examined by reverse transcription polymerase chain reaction (RT-PCR) and real-time quantitative RT-PCR.


RESULTS
The expression of RXRalpha mRNA was obviously decreased by NB-UVB irradiation, but not by acitretion single treatment. When combining acitretin treatment with NB-UVB irradiation, greater decreased RXRalpha mRNA expression was observed than that of single treatment.


CONCLUSIONS
Narrow-band UVB irradiation treatment can decrease RXRalpha mRNA expression, but not acitretin single treatment. Combining treatment with both can produce synergistic inhibition effects.
Luo
Su-Ju
SJ
Department of Dermatology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Zheng
Yan
Y
Peng
Zhen-Hui
ZH
Wang
Guo-Rong
GR
Zhou
Shao-Na
SN
Li
Xiao-Li
XL
D017255:Acitretin / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects / Q000528:radiation effects; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D047490:Retinoid X Receptor alpha / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014466:Ultraviolet Rays
D012333:RNA, Messenger; D047490:Retinoid X Receptor alpha; D017255:Acitretin