Acute exercise may exacerbate oxidative stress response in hemodialysis patients.
OBJECTIVE
Hemodialyzed patients (HD) demonstrate elevated oxidative stress (OXS) levels. Exercise effects on OXS response and antioxidant status of HD was investigated in the present study.


METHODS
Twelve HD and 12 healthy controls (HC) performed a graded exercise protocol. Blood samples, collected prior to and following exercise, were analyzed for lactate, thiobarbituric acid-reactive substances (TBARS), protein carbonyls (PC), reduced (GSH) and oxidized glutathione (GSSG), total antioxidant capacity (TAC), catalase, and glutathione peroxidase (GPX) activity.


RESULTS
HC demonstrated higher time-to-exhaustion (41%), lactate (41%) and VO2 peak (55%) levels. At rest, HD exhibited higher TBARS, PC, and catalase activity values and lower GSH, GSH/GSSG, TAC, and GPX levels. Although exercise elicited a marked change of OXS markers in both groups, these changes were more pronounced (p < 0.05) in HD patients. After adjusting for VO2 peak, differences between groups disappeared. VO2 peak was highly correlated with GSH/GSSG, TBARS, TAC and PC at rest and after exercise.


CONCLUSIONS
These results imply that HD demonstrate higher OXS levels and a lower antioxidant status than HC at rest and following exercise. Acute exercise appears to exacerbate OXS response in hemodialyzed patients probably due to diminished antioxidant defense. However, aerobic capacity level seems to be related to OXS responses in this population.
Fatouros
Ioannis G
IG
Department of Physical Education & Sport Science, Democritus University of Thrace, Komotini, Greece.
Pasadakis
Ploumis
P
Sovatzidis
Apostolos
A
Chatzinikolaou
Athanasios
A
Panagoutsos
Stylianos
S
Sivridis
Dimitrios
D
Michailidis
Ioannis
I
Douroudos
Ioannis
I
Taxildaris
Kiriakos
K
Vargemezis
Vasilios
V
D000328:Adult; D005080:Exercise Test; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010807:Physical Endurance; D017382:Reactive Oxygen Species / Q000097:blood*; D006435:Renal Dialysis; D051437:Renal Insufficiency / Q000097:blood / Q000503:physiopathology* / Q000534:rehabilitation*
D017382:Reactive Oxygen Species