E-cadherin core fucosylation regulates nuclear beta-catenin accumulation in lung cancer cells.
E-cadherin expressed highly in 95C and lowly in 95D lung cancer cells which were from the same patient, but core-fucosylated E-cadherin highly expressed in 95D cells. Therefore, Fut8 and Fut8-RNAi constructs were transfected into 95C and 95D cells, respectively. In Fut8-transfectants, reduction of nuclear beta-catenin was noted when E-cadherin was core-fucosylated, while accumulation of nuclear beta-catenin was observed in Fut8-RNAi transfectants. In E-cadherin-negative MDA-MB-231 cells either Fut8 or Fut8-RNAi transfection couldn't affect nuclear beta-catenin. However, cotransfection of E-cadherin with Fut8 caused nuclear beta-catenin reduction. Furthermore, enhanced binding of E-cadheirn with beta-catenin as well as alpha-catenin were observed in Fut8-transfectants, and reduction of tyrosine 654 phosphorylation on beta-catenin and its transcriptional activity were also noted at the same time. Overall, the current results suggested that core-fucosylated E-cadherin regulated nuclear beta-catenin accumulation in lung cancer cells.
Hu
Ping
P
Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, 138 Yi Xue Yuan Road, Shanghai, 200032, China.
Shi
Bizhi
B
Geng
Fei
F
Zhang
Chunyi
C
Wu
Wei
W
Wu
Xing Zhong
XZ
D015820:Cadherins / Q000378:metabolism*; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D005643:Fucose / Q000378:metabolism*; D005647:Fucosyltransferases / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000201:enzymology / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D019000:Phosphotyrosine / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D051820:TCF Transcription Factors / Q000235:genetics; D014158:Transcription, Genetic; D014162:Transfection; D051176:beta Catenin / Q000235:genetics / Q000378:metabolism*
D015820:Cadherins; D051820:TCF Transcription Factors; D051176:beta Catenin; D019000:Phosphotyrosine; D005643:Fucose; D005647:Fucosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase