Influence of bisphenol a on developing rat estrogen receptors and some cytokines in rats: a two-generational study.
Bisphenol A (BPA) is an industrial chemical with estrogenic activity. In our study, 40 rats were given BPA at 4, 40, and 400 mg/kg per day. Controls were treated with corn oil of same volume until the offspring were 30 d olds. At the end of the experiment, the expression of estrogen receptor (ER)-alpha and the mRNA levels of inerleukin (IL)-2, IL-12, interferon (IFN)-gamma, and tumor necrosis factor (TNF)-alpha in spleens were investigated by Western blotting and real-time reverse-transcription polymerase chain reaction (RT-PCR). The histopathological change of organs was observed. The results showed that bisphenol A reduced the expression of ER-alpha in males of the high-dose group in the F(0) generation and in middle- and high-dose groups of the F(1) generation (p < .05), but increased it in females in the high-dose group of the F(0) generation and in middle- and high-dose groups of the F(1) generation (p < .05). The levels of IL-2, IL-12, IFN-gamma, and TNF-alpha in spleens were downregulated in all groups in contrast to controls (p < .05). We also found some histological changes in spleens, livers, and kidneys. These findings demonstrated that bisphenol A has estrogen-like activity and might affect some immune organs and parameters.
Department of Public Health, Shandong University, Jinan, China.
Song
S
Miao
Zhixian
Z
Gao
Zengqiang
Z
Kou
Guifa
G
Xu
Cunju
C
Su
Nan
N
Liu
D000818:Animals; D001559:Benzhydryl Compounds; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D004968:Estrogens, Non-Steroidal / Q000633:toxicity*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D011247:Pregnancy; D012333:RNA, Messenger; D051381:Rats; D011916:Rats, Inbred F344; D011960:Receptors, Estrogen / Q000187:drug effects*; D013154:Spleen / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018675:Toxicity Tests; D000098826:Bisphenol A Compounds
D001559:Benzhydryl Compounds; D016207:Cytokines; D004968:Estrogens, Non-Steroidal; D010636:Phenols; D012333:RNA, Messenger; D011960:Receptors, Estrogen; C006780:bisphenol A; D000098826:Bisphenol A Compounds