Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis.
Although the Korean isolate KI-1 of Toxoplasma gondii has been considered to be a virulent type I lineage because of its virulent clinical manifestations, its genotype is unclear. In the present study, genotyping of the KI-1 was performed by multilocus PCR-RFLP and microsatellite sequencing. For 9 genetic markers (c22-8, c29-2, L358, PK1, SAG2, SAG3, GRA6, BTUB, and Apico), the KI-1 and RH strains exhibited typical PCR-RFLP patterns identical to the type I strains. DNA sequencing of tandem repeats in 5 microsatellite markers (B17, B18, TUB2, W35, and TgM-A) of the KI-1 also revealed patterns characteristic of the type I. These results provide strong genetic evidence that KI-1 is a type I lineage of T. gondii.
Department of Infection Biology, College of Medicine, Reseach Institute for Medical Science, Chungnam National University, Daejeon 301-747, Korea.
Juan-Hua
JH
Quan
Tae Yun
TY
Kim
In-Uk
IU
Choi
Young-Ha
YH
Lee
D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D016054:DNA, Protozoan / Q000235:genetics; D005838:Genotype; D007723:Korea; D051379:Mice; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D016415:Sequence Alignment; D014122:Toxoplasma / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*
D016054:DNA, Protozoan