Localization of myosin-Va in subpopulations of cells in rat endocrine organs.
Myosin-Va is a Ca(2+)/calmodulin-regulated unconventional myosin involved in the transport of vesicles, membranous organelles, and macromolecular complexes composed of proteins and mRNA. The cellular localization of myosin-Va has been described in great detail in several vertebrate cell types, including neurons, melanocytes, lymphocytes, auditory tissues, and a number of cultured cells. Here, we provide an immunohistochemical view of the tissue distribution of myosin-Va in the major endocrine organs. Myosin-Va is highly expressed in the pineal and pituitary glands and in specific cell populations of other endocrine glands, especially the parafollicular cells of the thyroid, the principal cells of the parathyroid, the islets of Langerhans of the pancreas, the chromaffin cells of the adrenal medulla, and a subpopulation of interstitial testicular cells. Weak to moderate staining has been detected in steroidogenic cells of the adrenal cortex, ovary, and Leydig cells. Myosin-Va has also been localized to non-endocrine cells, such as the germ cells of the seminiferous epithelium and maturing oocytes and in the intercalated ducts of the exocrine pancreas. These data provide the first systematic description of myosin-Va localization in the major endocrine organs of rat.
Instituto de Genética e Bioquímica, Laboratório de Bioquímica e Biologia Molecular, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil. foued@ufu.br
Foued S
FS
Espindola
Silmara R
SR
Banzi
Luciana K
LK
Calabria
Rodrigo J
RJ
Custódio
Ricardo A
RA
Oliveira
Leandro D
LD
Procópio
Andreia B P
AB
Lima
Jair P
JP
Cunha-Junior
Milton V
MV
Coelho
Iêda M L
IM
Guedes
Cláudia H
CH
Pellizzon
Roy E
RE
Larson
Enilza M
EM
Espreafico
D000311:Adrenal Glands / Q000166:cytology / Q000378:metabolism; D000818:Animals; D004702:Endocrine Glands / Q000166:cytology / Q000378:metabolism; D015151:Immunoblotting; D007515:Islets of Langerhans / Q000166:cytology / Q000378:metabolism; D018995:Myosin Heavy Chains / Q000378:metabolism*; D024701:Myosin Type V / Q000378:metabolism*; D010280:Parathyroid Glands / Q000166:cytology / Q000378:metabolism; D010870:Pineal Gland / Q000166:cytology / Q000378:metabolism; D010902:Pituitary Gland / Q000166:cytology / Q000378:metabolism; D051381:Rats; D013961:Thyroid Gland / Q000166:cytology / Q000378:metabolism; D014018:Tissue Distribution
C513081:Myo5a protein, rat; D024701:Myosin Type V; D018995:Myosin Heavy Chains