Molecular characterization and association analysis with production traits of the porcine INPP5F gene.
Inositol polyphosphate-5-phosphatase F (INPP5F) is one of the largest families of phosphoinositide phosphatases, 5-phosphatase. It contains a Sac domain whose amino acids are essential for inositol polyphosphate phosphatase activities. Here, we assigned the porcine INPP5F to SSC14q29 by using SCHP and IMpRH. Sequencing of PCR products from different breeds identified an A/G polymorphism in the last exon. The allele frequencies of this SNP showed that the Yorkshire and Duroc pigs have high G allele frequency, whereas the local pigs have high A allele frequency. Association analysis of the genotypes with growth and carcass traits found that different genotypes of INPP5F have significant differences in average daily gain (ADG) (P < 0.05) in Yorkshire pigs.
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, People's Republic of China.
Quan-Yong
QY
Zhou
Jiang-Nan
JN
Huang
Meng-Jin
MJ
Zhu
Shu-Hong
SH
Zhao
D000818:Animals; D001835:Body Weight / Q000235:genetics; D001947:Breeding; D033481:Chromosomes, Mammalian / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D000072181:Inositol Polyphosphate 5-Phosphatases; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics* / Q000254:growth & development*
D018076:DNA, Complementary; D010744:Phosphoric Monoester Hydrolases; C000606431:INPP5F protein, human; D000072181:Inositol Polyphosphate 5-Phosphatases