Micropatterning of bacteria on two-dimensional lattice protein surface observed by atomic force microscopy.
In this study, we characterized the two-dimensional lattice of bovine serum albumin (BSA) as a chemical and physical barrier against bacterial adhesion, using fluorescence microscopy and atomic force microscopy (AFM). The lattice of BSA on glass surface was fabricated by micro-contact printing (microCP), which is a useful way to pattern a wide range of molecules into microscale features on different types of substrates. The contact-mode AFM measurements showed that the average height of the printed BSA monolayer was 5-6 nm. Escherichia coli adhered rapidly on bare glass slide, while the bacterial adhesion was minimized on the lattices in the range of 1-3 microm(2). Especially, the bacterial adhesion was completely inhibited on a 1 microm(2) lattice. The results suggest that the anti-adhesion effects are due by the steric repulsion forces exerted by BSA.
Oh
Y J
YJ
Department of Physics and Division of Nano Sciences, Ewha Womans University, Seoul 120-750, Republic of Korea.
Jo
W
W
Lim
J
J
Park
S
S
Kim
Y S
YS
Kim
Y
Y
D000818:Animals; D001419:Bacteria / Q000378:metabolism*; D001422:Bacterial Adhesion; D002417:Cattle; D018625:Microscopy, Atomic Force / Q000379:methods*; D008856:Microscopy, Fluorescence; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013499:Surface Properties
D012710:Serum Albumin, Bovine