The effect of finasteride on spermatogenesis of Mesocricetus auratus.
OBJECTIVE
To study the effect of finasteride on the spermatogenesis of adult Mesocricetus auratus.


METHODS
Twenty adult hamsters were evaluated. The animals were one year-older, and were randomly divided in 2 different groups: control group with ten animals (n=10) and experimental group also with ten animals (n=10). The animals in the experimental group were shot 7.14 ng/mL (0.5mL) of finasteride by 100mg/Kg, subcutaneously in the dorsal region three times per week during 90 days. This dose corresponds to 5mg of the drug used in adult men for the treatment of benign prostatic hyperplasia (BPH). After three months, the animals were anesthetized through association of 200mg/kg ketamine chloridrate and 2.5 mg/kg of diazepan and were dead through hypovolemia.. The testis removed along with the whole genitourinary apparel were fixed with 10% formalin and submitted to histological analysis by optical microscopy. The hematoxilin-eosin (HE) method was used to stain the slides.


RESULTS
The mean weight of animals in the control group before death was 129.0+/-18.8gr. The mean weight of animals in experimental group was 145.0+/-15.25gr. The mean age of animals in control group before death was 15.2+/-1.13 months. The mean age of animals in experimental group before death was 17.16+/-0.82 months. The mean difference in weight between both groups was not statistical significant (p=0.0514). The totality of animals in control group (100%) presented no tubular alterations and showed no disturbancy in the spermatogenesis stages. Four animals (40%) in the experimental group showed hypotrophy of the seminiferous tubules and six (60%) showed normal spermatogenesis, however reduced compared to control group. There was statistically significant difference (p=0.043) between the control and experimental group related to testicular alterations.


CONCLUSIONS
The animals that were administered finasteride showed significant tubules atrophy and spermatogenesis reduction compared to control group.
Medicine School, Federal University of Minas Gerais, Brazil. dimas@barbacena.com.br
Dimas José Araújo
DJ
Vidigal
Alcino Lázaro da
AL
Silva
Luiz Mauro Andrade da
LM
Fonseca
Anilton Cesar
AC
Vasconcelos
Dilermando Fazito de
DF
Resende
Felipe Eduardo Costa
FE
Vidigal
D000818:Animals; D006224:Cricetinae; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D018120:Finasteride / Q000494:pharmacology*; D008297:Male; D008647:Mesocricetus; D011897:Random Allocation; D012671:Seminiferous Tubules / Q000187:drug effects / Q000473:pathology; D013091:Spermatogenesis / Q000187:drug effects*
D004791:Enzyme Inhibitors; D018120:Finasteride