Engineering motility as a phenotypic response to LuxI/R-dependent quorum sensing in Escherichia coli.
The repertoire of functional outputs interfaced with the LuxI/LuxR quorum sensing system in engineered Escherichia coli has been expanded to include motility via inducible expression of motB. Appropriate choice of ribosome binding site controlling MotB translation was crucial to achieving control over motility.
Weiss
Lucien E
LE
Badalamenti
Jonathan P
JP
Weaver
Lane J
LJ
Tascone
Anthony R
AR
Weiss
Paul S
PS
Richard
Tom L
TL
Cirino
Patrick C
PC
D054742:Acyl-Butyrolactones / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D002633:Chemotaxis / Q000235:genetics*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D011401:Promoter Regions, Genetic / Q000502:physiology; D053038:Quorum Sensing / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C085427:LuxI protein, Bacteria; C064779:MotB protein, Bacteria; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C064778:LuxR autoinducer binding proteins