Pretreatment of indian cane molasses for increased production of citric acid.
Surface culture citric acid fermentation was carried out by Aspergillus niger T55, a strain isolated from its natural source, using cane molasses, either untreated or treated by various methods. Citric acid biosynthesis is seriously impaired by both organic and inorganic inhibitors. A combined treatment of molasses with tricalcium phosphate, hydrochloric acid, and Sephadex fractionation minimizes the level of inorganic and organic inhibitors in molasses and increases the production of citric acid (65% weight yield based on total reducing sugar). The optimum level of individual metal ions for citric acid production depends on the concentration of other metals in the medium.
Kundu
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Biochemical Engineering Research Centre, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi-110016, India.
Panda
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Majumdar
S K
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Guha
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Bandyopadhyay
K K
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