Early expression of interleukin-2 mRNA by peripheral blood mononuclear cells isolated from nickel-allergic subjects and subsequently exposed to nickel in vitro.
The aim pf the present study was to characterize the time course of the synthesis of mRNA encoding interleukin-2 (IL-2) by peripheral blood mononuclear cells (PBMCs) isolated from nickel-allergic women and subsequently exposed to nickel sulphate in vitro. The level of this mRNA (determined by employing real-time polymerase chain reaction [PCR]) and of the corresponding protein (quantitated using an enzyme-linked immunosorbent assay [ELISA]) were significantly increased after only 10 and 24 h of incubation, respectively. The increased level of mRNA coding for IL-2 supports the hypothesis that this process is a key part of the response to nickel stimulation of PBMCs of hypersensitive subjects.
Unit of Dermatology and Venereology, Department of Medicine, Karolinska University Hospital, Stockholm, Sweden.
Hale
H
Falsafi-Amin
Mikael
M
Holst
Lena
L
Lundeberg
D000328:Adult; D000368:Aged; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000276:immunology; D007509:Irritants / Q000276:immunology / Q000494:pharmacology*; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism*; D008875:Middle Aged; D009532:Nickel / Q000276:immunology / Q000494:pharmacology*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000276:immunology; D013997:Time Factors
C508594:IL2 protein, human; D007376:Interleukin-2; D007509:Irritants; D012333:RNA, Messenger; C029938:nickel sulfate; D009532:Nickel