New finding of Giardia intestinalis (Eukaryote, Metamonad) in Old World archaeological site using immunofluorescence and enzyme-linked immunosorbent assays.
In this study, nine organic sediment samples from a medieval archaeological site at Pineuilh, France, were examined for Giardia intestinalis using two commercially available immunological kits [enzyme-linked immuno sorbent and immunofluorescence (IFA) assays]. Both techniques detected G. intestinalis in one sample, dated to 1,000 Anno Domini. This is the first time IFA was successfully used to detect protozoa in Old World archaeological samples. Such immunological techniques offer important perspectives concerning ancient protozoa detection and identification.
Laboratoire de Paléoparasitologie, UFR de Pharmacie, Université de Reims, Reims, France. matthieu.lebailly@univ-reims.fr
Matthieu
M
Le Bailly
Marcelo Lc
ML
Gonçalves
Stéphanie
S
Harter-Lailheugue
Frédéric
F
Prodéo
Adauto
A
Araujo
Françoise
F
Bouchet
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D005602:France; D005872:Giardia / Q000302:isolation & purification*; D006801:Humans; D010164:Paleopathology