[Levels of prostaglandin E2 (PGE2) in cervical ovulatory mucus in women with spontaneous miscarriages].
OBJECTIVE
The aim of this study was to compare levels of PGE2 in cervical ovulatory mucus in patients with various frequency of spontaneous miscarriages, and in healthy fertile women.


METHODS
Special Consultation for Immunology of Reproduction and Research Laboratories for Reproductive Immunology, Department of Gynaecology and Obstetrics, Medical Faculty of Charles University and Faculty Hospital, Pilsen.


METHODS
We used commercial, high sensitive method of ELISA (PGE2 HS, R&D Systems) for detection of PGE2 in cervical ovulatory mucus. Statistic analysis (T-test, ANOVA-test and Kruskal-Wallis test) also provided our study.


RESULTS
It was taken in total 120 measurements. The R & D Systems company does not mention physiological levels of PGE2 in cervical ovulatory mucus. Therefore we compared all results of our studied groups with average concentration (197356 pg/ml PGE2) gained from control group. We found that in patients with one miscarriage PGE2 levels are approximately 6 times higher, with two miscarriages 13 times higher, and in patients with three and more miscarriages even 21 times higher than controls (p < 0.0327, alpha 5%).


CONCLUSIONS
In present literature does not exist any study concerning of PGE2 in cervical ovulatory mucus. We prove that some patients mainly with repeated "unexplained" miscarriages have ovulatory levels of PGE2 significantly higher than controls.
Gynekologicko-porodnická klinika LF UK a FN, Plzen.
M
M
Ryantová
Z
Z
Ulcová-Gallová
Z
Z
Micanová
K
K
Bibková
B
B
Sedivá
D000022:Abortion, Spontaneous / Q000378:metabolism*; D000328:Adult; D002582:Cervix Mucus / Q000737:chemistry*; D015232:Dinoprostone / Q000032:analysis*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000378:metabolism*; D010060:Ovulation; D011247:Pregnancy
D015232:Dinoprostone