Effect of sulfite treatment on total antioxidant capacity, total oxidant status, lipid hydroperoxide, and total free sulfydryl groups contents in normal and sulfite oxidase-deficient rat plasma.
Sulfites, which are commonly used as preservatives, are continuously formed in the body during the metabolism of sulfur-containing amino acids. Sulfite oxidase (SOX) is an essential enzyme in the pathway of the oxidative degradation of sulfite to sulfate protecting cells from sulfite toxicity. This article investigated the effect of sulfite on total antioxidant capacity (TAC), total oxidant status, lipid hydroperoxide (LOOH), and total free sulfydryl groups (-SH) levels in normal and SOX-deficient male albino rat plasma. For this purpose, rats were divided into four groups: control, sulfite-treated, SOX-deficient, and sulfite-treated SOX-deficient groups. SOX deficiency was established by feeding rats a low molybdenum diet and adding to their drinking water 200 ppm tungsten. Sulfite (70 mg/kg) was administered to the animals via their drinking water. SOX deficiency together with sulfite treatment caused a significant increase in the plasma LOOH and total oxidant status levels. -SH content of rat plasma significantly decreased by both sulfite treatment and SOX deficiency compared to the control. There was also a significant decrease in plasma TAC level by sulfite treatment. In conclusion, sulfite treatment affects the antioxidant/oxidant balance of the plasma cells of the rats toward oxidants in SOX-deficient groups.
Faculty of Engineering, Department of Food Engineering, Pamukkale University, Kinikli 20020, Denizli, Turkey. nurherken@pau.edu.tr
Emine Nur
EN
Herken
Erdogan
E
Kocamaz
Ozcan
O
Erel
Hakim
H
Celik
Vural
V
Kucukatay
D000818:Animals; D005520:Food Preservatives / Q000494:pharmacology*; D005609:Free Radicals / Q000097:blood; D008054:Lipid Peroxides / Q000097:blood*; D008297:Male; D010084:Oxidation-Reduction; D051381:Rats; D013438:Sulfhydryl Compounds / Q000097:blood*; D050876:Sulfite Oxidase / Q000097:blood* / Q000172:deficiency; D013447:Sulfites / Q000494:pharmacology*
D005520:Food Preservatives; D005609:Free Radicals; D008054:Lipid Peroxides; D013438:Sulfhydryl Compounds; D013447:Sulfites; C005200:sodium metabisulfite; D050876:Sulfite Oxidase