Deoxynivalenol: rationale for development and application of a urinary biomarker.
Mycotoxins are common dietary contaminants in most regions of the world. The frequency of exposure to the various families of mycotoxins is often dependent on geographic location, national wealth and related agricultural and regulatory infrastructure, combined with diversity of diet and degree of food sufficiency. Deoxynivalenol (DON) is a Fusarium mycotoxin that frequently contaminates wheat, corn and barley in temperate regions. A number of acute poisoning incidences have been linked to DON-contaminated foods and chronic exposure to lower levels of DON has been predicted in many regions. DON is a potent animal toxin and exposure in humans may cause gastroenteritis, growth faltering and immune toxicity. An ability to conduct accurate exposure assessment at the individual level is required to fully understand the potential health consequences for humans. To date, such exposure biomarkers have been lacking for many important mycotoxins, including DON. To better assess exposure to DON at the individual level, we have developed a robust urinary assay, incorporating immunoaffinity column (IAC) enrichment and LC-MS detection. Further refinement of this urinary assay, by inclusion of (13)C-DON as an internal standard, was then undertaken and tested within the UK. DON was frequently observed in urine and was significantly associated with cereal intake. A dietary intervention study demonstrated that avoiding wheat in the diet markedly reduced urinary levels of DON. This biomarker requires further validation but our initial data suggest it may provide a useful tool in epidemiological investigations of the potential health consequences of this common environmental toxin.
Molecular Epidemiology Unit, Centre for Epidemiology and Biostatistics, Leeds Institute of Genetics, Health and Therapeutics, Faculty of Medicine and Health, University of Leeds, Leeds LS2 9JJ, UK. p.c.turner@leeds.ac.uk
Paul C
PC
Turner
Victoria J
VJ
Burley
Joseph A
JA
Rothwell
Kay L M
KL
White
Janet E
JE
Cade
Christopher P
CP
Wild
D015415:Biomarkers / Q000652:urine; D002853:Chromatography, Liquid / Q000379:methods; D002523:Edible Grain / Q000737:chemistry* / Q000382:microbiology; D005506:Food Contamination / Q000032:analysis*; D005670:Fusarium / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D009183:Mycotoxins / Q000633:toxicity / Q000652:urine*; D013223:Statistics as Topic; D014255:Trichothecenes / Q000032:analysis / Q000652:urine*
D015415:Biomarkers; D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol