Short-end injection capillary electrophoresis for quantification of plasma chondroitin sulfate isomer disaccharides.
We describe a new ultra-rapid capillary electrophoresis method with UV detection for analysis of the disaccharides obtained after enzymatic depolymerization of plasma chondroitin sulfates. The free reducing groups of the released carbohydrate molecules are derivatized with 2-aminoacridone by reductive amination in the presence of cyanoborohydride. The fluorotagged products can be separated by short-end injection capillary electrophoresis in a capillary with an effective length of 10.2 cm. The migration times of Delta di-0S and Delta di-4S were 0.95 and 1.81 min, respectively. We compared the proposed method with UV detection to a reference CE-LIF assay by measuring plasma chondroitin sulfate in 94 subjects. The described assay for total plasma CS measurement may, owing to the high throughput and the fast analytical times, be a good tool for routine studies both in research and in clinical applications.
Department of Biomedical Sciences, University of Sassari, Viale San Pietro, 43/B-07100, Sassari, Italy. angelozinellu@libero.it
Angelo
A
Zinellu
Salvatore
S
Sotgia
Maria Franca
MF
Usai
Elisabetta
E
Zinellu
Antonio J
AJ
Lepedda
Luca
L
Deiana
Marilena
M
Formato
Ciriaco
C
Carru
D000328:Adult; D002809:Chondroitin Sulfates / Q000097:blood* / Q000737:chemistry*; D004187:Disaccharides / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D007536:Isomerism; D008297:Male; D008875:Middle Aged; D013056:Spectrophotometry, Ultraviolet; D013997:Time Factors
D004187:Disaccharides; D005456:Fluorescent Dyes; D002809:Chondroitin Sulfates