[Analysis of human metapneumovirus genotype in Hunan, China and its attachment protein G sequence character].
OBJECTIVE
To understand the genotypes of human metapneumovirus (hMPV) and the genetic character of hMPV attachment protein G sequence in Hunan, China.


METHODS
232 nasopharyngeal aspirates (NPA) samples from hospitalized children with acute respiratory infections were collected from Hunan, China in 2005. HMPV was detected. The full length of G glycoprotein genes were amplified and sequenced. Bioinformatics soft-wares were employed to analyze the sequences.


RESULTS
17/232 (7.3%) were showed hMPV positive. And co-infection rate with other viruses is 35%. The diagnoses of these hMPV positive cases are pneumonia, bronchiolitis and bronchopneumonia. Phylogenetic analysis for G genes from 13 hMPVs revealed the existence of four major subgroups: A1, A2, B1, B2 in Hunan, China in 2005. There are four types of sequence lengths of hMPV G glycoprotein, which are 711, 675, 660, 696nt. It is different in potential N-linked glycosylation sites and number of cysteine residues among these hMPVs of Hunan, China and Beijing, China. Also it is different from those in Japan and North America.


CONCLUSIONS
The investigation of hMPV from Hunan, China in 2005 revealed the high speed of genetic variation and the marked character of geographic epidemic differences.
National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China.
Wen-pei
WP
Liu
Bing
B
Zhang
Zhi-ping
ZP
Xie
Li-li
LL
Zhong
Xiao-wang
XW
Qu
Li-shu
LS
Zheng
Han-chun
HC
Gao
Zheng-yu
ZY
Qi
Zhi-zhou
ZZ
Kuang
Wan-ju
WJ
Zhang
Zhou-jun
ZJ
Duan
Yun-de
YD
Hou
D000595:Amino Acid Sequence; D002648:Child; D002681:China / Q000453:epidemiology; D005838:Genotype; D006023:Glycoproteins / Q000145:classification / Q000235:genetics*; D006801:Humans; D029121:Metapneumovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D018357:Respiratory Syncytial Virus Infections / Q000453:epidemiology / Q000821:virology*; D017386:Sequence Homology, Amino Acid; D014764:Viral Proteins / Q000145:classification / Q000235:genetics*
C484233:G glycoprotein, human metapneumovirus; D006023:Glycoproteins; D012367:RNA, Viral; D014764:Viral Proteins