Screening of Aflatoxins and Zearalenone in feedstuffs and complete feeds for pigs in Southern Vietnam.
A survey ofaflatoxins and zearalenone (ZEA) in 120 samples, including cassava chip (12), maize (12), rice bran (24), broken rice (24), and complete feeds for growing pigs (24) and sows (24) was carried out in southern Vietnam. Aflatoxin B1 (AFB1), B2 (AFB2), G1 (AFG1) and G2 (AFG2) were determined by an HPLC method using an immunoaffinity column for clean-up. Zearalenone was analysed by Enzyme-Linked Immunosorbent Assay (ELISA) AgraQuant. High incidences of aflatoxins and ZEA in feedstuffs and pig feeds (83.3%-100%) were detected. Aflatoxins and ZEA were detected in all commodities analyzed. Aflatoxin B1 was found in 100, 92, 92, 83, 100 and 96%, respectively, of samples of cassava chip, maize, rice bran, broken rice, and complete feeds for growing pigs and sows, with mean concentrations of 0.86, 77.5, 1.3, 1.6, 4.7 and 7.5 microg/kg, respectively. AFB2 and AFG1 were found in low concentrations and co-occurred with AFB1 in all samples where they were found. ZEA was detected in 8%, 33%, 36%, 21%, 67% and 67%, respectively, of the samples of cassava chip, maize, rice bran, broken rice, and complete feeds for growing pigs and sows, with average concentrations of 10.0, 163.5, 83.1, 31.4, 86.0 and 101.4 microg/kg, respectively. The high incidence of mycotoxins found in feedstuffs highlights the need for periodic monitoring and prevention ofmycotoxins, not only in feeds, but also in food for human consumption.
Department of Animal Nutrition, Nong Lam University, Linhtrung hamlet Thuduc district, Hochiminh City, Vietnam. qthieu68@yahoo.com
Nguyen Quang
NQ
Thieu
Brian
B
Ogle
Hans
H
Pettersson
D000348:Aflatoxins / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D002846:Chromatography, Affinity; D004797:Enzyme-Linked Immunosorbent Assay; D005506:Food Contamination / Q000032:analysis*; D013552:Swine; D003313:Zea mays / Q000737:chemistry; D015025:Zearalenone / Q000032:analysis*
D000348:Aflatoxins; D015025:Zearalenone