Morphological diagnosis of infective larvae of Libyostrongylus douglassii (Cobbold, 1882) Lane, 1923 and L. dentatus Hoberg, Lloyd and Omar, 1995 (Nematoda: Trichostrongylidae) of ostriches.
The differentiation of the species of the Libyostrongylus genus is only possible with the obtainment of the adult parasites in the ostriches proventriculus and gizzard. The present work confirms that it is possible to differentiate the infective larvae of L. douglassii and L. dentatus allowing the differential diagnosis of these species by fecal culture. To show this, adult females from both species were collected from ten proventriculus from adult ostriches and separated by species. Both groups were macerated individually added to sterilized feces for standard fecal cultures. The infective larvae were recovered, identified, quantified and measured. All proventriculus analyzed were parasitized by Libyostrongylus spp. and a clear heterogeneous location for each species was observed. The infective larvae from the fecal cultures of macerated L. douglassii presented a mean total length of 874.3+/-33.80 microm, and a short sheath tail (29.5+/-4.11 microm) with acute termination. The infective larvae from the macerated L. dentatus presented mean total length of 856.0+/-43.63 microm, long sheath tail (61.2+/-9.52 microm) with filamentous termination. The mean measures of the tails of both species had a significant difference. The differentiation of the infective larvae of L. douglassii and L. dentatus by fecal cultures will facilitate the diagnosis of both species for further understanding the Libyostrongylus biology.
Laboratório de Helmintologia, Departamento de Parasitologia Animal (DPA), Instituto de Veterinária (IV), Universidade Federal Rural do Rio de Janeiro (UFRRJ), 23890-000 Seropédica, RJ, Brazil.
Nicole Brand
NB
Ederli
Francisco Carlos Rodrigues
FC
de Oliveira
Carlos Wilson Gomes
CW
Lopes
Renato Augusto
RA
Damatta
Clóvis de Paula
Cde P
Santos
Maria de Lurdes
Mde L
de Azevedo Rodrigues
D000818:Animals; D001715:Bird Diseases / Q000175:diagnosis* / Q000469:parasitology; D005243:Feces / Q000469:parasitology; D005260:Female; D007814:Larva / Q000033:anatomy & histology / Q000648:ultrastructure; D013045:Species Specificity; D020310:Struthioniformes / Q000469:parasitology*; D014251:Trichostrongyloidea / Q000033:anatomy & histology* / Q000302:isolation & purification / Q000648:ultrastructure; D014252:Trichostrongyloidiasis / Q000175:diagnosis / Q000662:veterinary*