Acute expression of hepatitis C core protein in adult mouse liver: Mitochondrial stress and apoptosis.
OBJECTIVE
In infection with hepatitis C virus (HCV), spontaneous clearance of the virus occurs in 30-40% of cases. By contrast, in chronic infection, this is rare. The basis for viral clearance in acute disease is unknown. Whereas cellular immune responses have been studied in detail, few data exist on the role of viral structural proteins, such as the core protein. The purpose of this study was to investigate the effects of core produced de novo within adult mouse hepatocytes by using a new transgenic mouse line in which expression of HCV core is regulated by tetracycline (tet-off).


METHODS
In this work, transgenic mice with conditional HCV core were created, to study the acute expression of HCV core protein in the context of the mature liver. The subcellular distribution of the core, hepatocellular oxidative stress and apoptosis were monitored.


RESULTS
Core protein is readily detectable and strongly associated with cytoplasmic lipid vesicles, endoplasmic reticulum and mitochondria. Mitochondrial oxidative stress was evidenced by a reduction in thioredoxin-2 (trx2). Concurrently, caspase-3 activity and TUNEL increased and, over time, the level of core protein in the liver declined.


CONCLUSIONS
Mice that are conditionally transgenic for HCV core protein, which is readily detected and morphologically associated with steatosis in individual hepatocytes, were developed. Acute expression of core protein causes mitochondrial stress, as demonstrated by a reduction in trx2 and in the apoptosis of core-positive hepatocytes. We speculate that these events could be involved in the clearance of virus during acute hepatitis C, by both reducing the burden of virus in the liver and effectively priming the immune response.
Liver Research Center and Department of Hepatogastroenterology, Chang Gung Memorial Hospital, Chang Gung University, College of Medicine, Taoyuan, Taiwan. mlchang8210@gmail.com
Ming-Ling
ML
Chang
Jeng-Chang
JC
Chen
Ming-Yu
MY
Chang
Chau-Ting
CT
Yeh
Wei-Pin
WP
Lin
Chun-Kai
CK
Liang
Shiu-Feng
SF
Huang
Kim N
KN
Dang
Cheng-Tang
CT
Chiu
Deng-Yn
DY
Lin
D000818:Animals; D017209:Apoptosis; D053148:Caspase 3 / Q000378:metabolism; D053453:Caspase 9 / Q000378:metabolism; D005234:Fatty Liver / Q000378:metabolism / Q000473:pathology / Q000821:virology; D018936:Hepatitis C Antigens / Q000378:metabolism*; D022781:Hepatocytes / Q000378:metabolism; D007150:Immunohistochemistry; D020287:In Situ Nick-End Labeling; D008099:Liver / Q000378:metabolism* / Q000473:pathology / Q000821:virology; D051379:Mice; D008822:Mice, Transgenic; D008930:Mitochondria, Liver / Q000378:metabolism*; D015388:Organelles / Q000378:metabolism; D018384:Oxidative Stress; D013879:Thioredoxins / Q000378:metabolism; D014758:Viral Core Proteins / Q000378:metabolism*
D018936:Hepatitis C Antigens; D014758:Viral Core Proteins; C069130:nucleocapsid protein, Hepatitis C virus; D013879:Thioredoxins; D053148:Caspase 3; D053453:Caspase 9