Single molecule genotyping by TIRF microscopy.
As part of a programme to develop a metrological framework for single molecule measurements in biology, we have investigated the applications of single molecule imaging to genomics. Specifically, we have developed a technique for measuring the frequencies of single nucleotide polymorphisms (SNPs) in complex or pooled samples of DNA. We believe that this technique has applications to statistical genotyping-the identification of correlations between SNP frequencies and particular phenotypes-and other areas where it is desirable to track the frequencies of SNPs in complex DNA populations.
Biomedical Optics Department, Physikalisch-Technische Bundesanstalt, Berlin, Germany.
Steffen
S
Rüttinger
Baptiste
B
Lamarre
Alex E
AE
Knight
D000483:Alleles; D004247:DNA / Q000235:genetics; D005787:Gene Frequency; D023281:Genomics; D005838:Genotype; D006801:Humans; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D010957:Plasmids / Q000235:genetics; D020641:Polymorphism, Single Nucleotide; D017422:Sequence Analysis, DNA
D004247:DNA