Efficient and selective enzymatic acylation reaction: separation of furanosyl and pyranosyl nucleosides.
Candida antarctica lipase-B (CAL-B) immobilized on lewatite selectively acylated the primary hydroxyl group of the furanosyl nucleoside in a mixture of 1-(alpha-D-arabinofuranosyl)thymine and 1-(alpha-D-arabinopyranosyl)thymine. This selective biocatalytic acylation of furanosyl nucleoside has enabled us an easy separation of arabinofuranosyl thymine from an inseparable mixture with arabinopyranosyl thymine. The primary hydroxyl selective acylation methodology of arabinonucleoside has also been successfully used for the separation of 1-(beta-D-xylofuranosyl)thymine and 1-(beta-D-xylopyranosyl)thymine from a mixture of the two, which demonstrate the generality of the enzymatic methodology for separation of furanosyl and pyranosyl nucleosides.
Maity
Jyotirmoy
J
Bioorganic Laboratory, Department of Chemistry, University of Delhi, Delhi-110 007, India.
Shakya
Gaurav
G
Singh
Sunil K
SK
Ravikumar
Vasulinga T
VT
Parmar
Virinder S
VS
Prasad
Ashok K
AK
D000215:Acylation; D002384:Catalysis; D005656:Fungal Proteins; D005663:Furans / Q000737:chemistry*; D008049:Lipase / Q000378:metabolism*; D015394:Molecular Structure; D009705:Nucleosides / Q000737:chemistry* / Q000378:metabolism*; D011714:Pyrans / Q000737:chemistry*
D005656:Fungal Proteins; D005663:Furans; D009705:Nucleosides; D011714:Pyrans; D008049:Lipase; C431349:lipase B, Candida antarctica