Effect of alcohol consumption on the liver detoxication capacity as measured by [13C]methacetin- and [methyl-13C]methionine-breath tests.
The aim of this study was to investigate the hepatic microsomal and mitochondrial functions by using the 13CO2-breath test in healthy subjects either before or after the consumption of red wine. Fourteen adults received [13C]methacetin and [methyl-13C]methionine together with a standardised dinner. Expired air samples were taken over 6 h. After a wash-out period, the subjects consumed 0.4 ml ethanol/kg/day together with dinner over a 10-day period. Thereafter, 13C-tracer administration was repeated under identical conditions. The 13CO2-enrichments were measured by isotope ratio mass spectrometry. The mean cumulative percentage 13C-dose recovery (CPDR) after administration of [13C]methacetin and [methyl-13C]methionine either without or with red wine consumption amounted to 38.2+/-6.3 vs. 36.3+/-6.7% (p=0.363) and 9.5+/-3.3 vs. 8.8+/-2.5% (p=0.47), respectively. Moderate alcohol consumption does not induce significant short-term changes of the microsomal and the mitochondrial functions of the human liver in healthy subjects.
Wutzke
Klaus D
KD
Children's Hospital, Research Laboratory 'Gastroenterology and Nutrition', University of Rostock, Rostock, Germany. klaus-dieter.wutzke@med.uni-rostock.de
Forberger
Anke
A
Wigger
Marianne
M
D000328:Adult; D000428:Alcohol Drinking / Q000378:metabolism*; D001944:Breath Tests / Q000379:methods*; D002247:Carbon Isotopes / Q000032:analysis*; D018592:Cross-Over Studies; D000431:Ethanol / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D050216:Metabolic Detoxication, Phase I / Q000502:physiology*; D008862:Microsomes, Liver / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D014920:Wine
D002247:Carbon Isotopes; D000431:Ethanol