Antimicrobial activity of dexamethasone and its combination with N-chlorotaurine.
OBJECTIVE
To investigate the antimicrobial effect of dexamethasone phosphate, the endogenous antiseptic N-chlorotaurine (NCT), and their combination on ear, nose, and throat microorganisms.


METHODS
In vitro study.


METHODS
Strains of Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus milleri, Aspergillus flavus, and Aspergillus fumigatus.


METHODS
Bacterial and fungal strains were cultured with 0.1% dexamethasone with and without a low (0.1%) or high (1%) concentration of NCT. The killing effects of dexamethasone, NCT, and the combination were monitored.


RESULTS
Dexamethasone killed S. milleri and A. flavus after incubation times of 24 to 48 hours. The low concentration of NCT caused a 90% reduction of S. aureus and P. aeruginosa within 30 minutes and 99.9% reduction within 50 minutes. The high concentration of NCT reduced viable counts of S. aureus and P. aeruginosa to the detection limit within 10 minutes. The low-concentration combination (0.1% dexamethasone and 0.1% NCT) showed significant (P < .01) synergistic killing of S. aureus with 2- to 3-fold shorter killing times. The high-concentration combination (0.1% dexamethasone and 1% NCT) demonstrated more rapid killing than NCT alone in both S. aureus and P. aeruginosa.


CONCLUSIONS
With short and intermediate exposure times, the combination of dexamethasone and NCT showed significantly stronger antimicrobial effects than treatment with NCT alone. Significant killing of S. milleri, A. flavus, and A. fumigatus was observed after extended exposure to dexamethasone. The combined application of dexamethasone and NCT might be a promising therapeutic option, producing high efficacy with low side effects.
Neher
Andreas
A
Department of Otorhinolaryngology, Innsbruck Medical University, Anichstr 35, Innsbruck, Austria. Andreas.Neher@i-med.ac.at
Arnitz
Roland
R
Gstöttner
Michaela
M
Schäfer
Dirk
D
Kröss
Eva-Maria
EM
Nagl
Markus
M
D000890:Anti-Infective Agents / Q000494:pharmacology*; D001231:Aspergillus flavus / Q000187:drug effects; D001232:Aspergillus fumigatus / Q000187:drug effects; D001419:Bacteria / Q000187:drug effects*; D003907:Dexamethasone / Q000494:pharmacology*; D004359:Drug Therapy, Combination; D005658:Fungi / Q000187:drug effects*; D006801:Humans; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D034364:Streptococcus milleri Group / Q000187:drug effects; D013654:Taurine / Q000031:analogs & derivatives* / Q000494:pharmacology
D000890:Anti-Infective Agents; D013654:Taurine; C043410:N-chlorotaurine; D003907:Dexamethasone