[Effect of high frequency oscillatory ventilation combined with exogenous pulmonary surfactant on apoptosis of lung tissue in rabbit with acute respiratory failure induced by steam inhalation injury].
OBJECTIVE
To investigate the effect of high frequency oscillatory ventilation (HFOV) combined with exogenous pulmonary surfactant (PS) on apoptosis of lung tissue in rabbit suffering from steam inhalation injury.


METHODS
Thirty-two New Zealand rabbits were randomly divided into four groups, and acute respiratory failure model was reproduced by severe steam inhalation injury. Then they were ventilated and treated by controlled mandatory ventilation (CMV), HFOV, CMV+PS or HFOV+PS, respectively. After the animals were euthanized at 4 hours treatment time point, the right middle lobe of rabbit lung was harvested for the examination of caspase-3 and p73 contents and relative values, as well as mRNA expression of caspase-3 and p73.


RESULTS
(1)Contents of caspase-3 and p73 in lung tissue homogenates in HFOV group and HFOV+PS group were significantly lower than those in CMV group and CMV+PS group (P<0.05 or P<0.01), and also contents of caspase-3 and p73 in lung tissue homogenates in groups with exogenous PS were significantly lower than those of groups without exogenous PS (all P<0.05). (2) Caspase-3, p73 mRNA expressions in lung tissue in HFOV group and HFOV+PS group were significantly lower than those of CMV group and CMV+PS group (all P<0.01). Caspase-3 and p73 mRNA expression in lung tissue in groups with exogenous PS group were significantly lower than those of the corresponding group without exogenous PS (all P<0.05).


CONCLUSIONS
Compared with CMV or CMV+PS, HFOV or HFOV+PS can decrease the concentration of caspase-3 and p73, reduce the mRNA expression of caspase-3 and p73 in the lung homogenates, as a result lung tissue apoptosis in inhalation injury may be suppressed.
Guo
Guang-Hua
GH
Department of Burn, The First Hospital Affiliated to Nanchang University, Nanchang 330006, Jiangxi, China. guogh2000@hotmail.com
Wang
Shao-Gen
SG
Fu
Zhong-Hua
ZH
Wu
Xing-Heng
XH
Yang
Ming
M
Chen
Xiao
X
D000818:Animals; D017209:Apoptosis; D002059:Burns, Inhalation / Q000378:metabolism / Q000473:pathology / Q000628:therapy; D053148:Caspase 3 / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D004195:Disease Models, Animal; D006612:High-Frequency Ventilation; D008168:Lung / Q000378:metabolism / Q000473:pathology; D009687:Nuclear Proteins / Q000378:metabolism*; D011663:Pulmonary Surfactants / Q000494:pharmacology*; D011817:Rabbits; D012128:Respiratory Distress Syndrome / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins / Q000378:metabolism*
D004268:DNA-Binding Proteins; D009687:Nuclear Proteins; D011663:Pulmonary Surfactants; D000072160:Tumor Protein p73; D025521:Tumor Suppressor Proteins; D053148:Caspase 3