Protein reactions with surface-bound molecular targets detected by oblique-incidence reflectivity difference microscopes.
We applied oblique-incidence reflectivity difference microscopes (a form of polarization-modulated nulling ellipsometry) to detection of biomolecular microarrays without external labeling in a study of protein reactions with surface-immobilized targets. We show that the optical reflectivity difference signals can be quantitatively related to changes in surface mass density of molecular layers as a result of the reactions. Our experimental results demonstrate the feasibility of using oblique-incidence reflectivity difference microscopes for high-throughput proteomics research such as screening unlabeled protein probes against libraries of surface-immobilized small molecules.
Department of Physics, University of California, Davis, CA 95616, USA.
J P
JP
Landry
Y S
YS
Sun
X W
XW
Guo
X D
XD
Zhu
D000818:Animals; D002417:Cattle; D006801:Humans; D007074:Immunoglobulin G / Q000378:metabolism*; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D040081:Protein Array Analysis / Q000379:methods; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000378:metabolism*; D013499:Surface Properties
D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine