Identification of a minimal functional linker in human topoisomerase I by domain swapping with Cre recombinase.
Cellular forms of type IB topoisomerases distinguish themselves from their viral counterparts and the tyrosine recombinases to which they are closely related by having rather extensive N-terminal and linker domains. The functions and necessity of these domains are not yet fully unraveled. In this study we replace 86 amino acids including the linker domain of the cellular type IB topoisomerase, human topoisomerase I, with four, six, or eight amino acids from the corresponding short loop region in Cre recombinase. In vitro characterization of the resulting chimeras, denoted Cropos, reveals that six amino acids from the Cre linker loop constitute the minimal length of a functional linker in human topoisomerase I.
Department of Molecular Biology and Interdisciplinary Nanoscience Center (iNANO), University of Aarhus, Denmark.
Rikke From
RF
Frøhlich
Sissel
S
Juul
Maria Bjerre
MB
Nielsen
Maria
M
Vinther
Christopher
C
Veigaard
Marianne Smedegaard
MS
Hede
Félicie Faucon
FF
Andersen
D002166:Camptothecin / Q000494:pharmacology; D004247:DNA / Q000378:metabolism; D004264:DNA Topoisomerases, Type I / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D019426:Integrases / Q000737:chemistry* / Q000378:metabolism*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology; D013329:Structure-Activity Relationship
D011994:Recombinant Proteins; D012965:Sodium Chloride; D004247:DNA; D006861:Hydrogen Peroxide; C045073:Cre recombinase; D019426:Integrases; D004264:DNA Topoisomerases, Type I; D002166:Camptothecin