Isolation and characterization of the Larix gmelinii ANGUSTIFOLIA (LgAN) gene.
ANGUSTIFOLIA (AN), a plant homolog of C-terminal binding protein, controls the polar elongation of leaf cells and the trichome-branching pattern in Arabidopsis thaliana. In the present study, degenerate PCR was used to isolate an ortholog of AN, referred to as LgAN, from larch (Larix gmelinii). The LgAN cDNA is predicted to encode a protein of 646 amino acids that shows striking sequence similarity to AN proteins from other plants. The predicted amino acid sequence has a conserved NAD-dependent 2-hydroxy acid dehydrogenase (D2-HDH) motif and a plant AN-specific LxCxE/D motif at its N-terminus, as well as a plant-specific long C-terminal region. The LgAN gene is a single-copy gene that is expressed in all larch tissues. Expression of the LgAN cDNA rescued the leaf width and trichome-branching pattern defects in the angustifolia-1 (an-1) mutant of Arabidopsis, showing that the LgAN gene has effects complementary to those of AN. These results suggest that the LgAN gene has the same function as the AN gene.
Graduate School of Science and Technology, Kumamoto University, Kurokami 2-39-1, Kumamoto, 860-8555 Japan. linxiaofei04@hotmail.com
Xiaofei
X
Lin
Naoko
N
Minamisawa
Katsuaki
K
Takechi
Wenbo
W
Zhang
Hiroshi
H
Sato
Susumu
S
Takio
Hirokazu
H
Tsukaya
Hiroyoshi
H
Takano
D000595:Amino Acid Sequence; D000818:Animals; D017796:Caulimovirus / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant / Q000235:genetics*; D006801:Humans; D028221:Larix / Q000033:anatomy & histology / Q000235:genetics*; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D010802:Phylogeny; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics*; D010957:Plasmids; D018749:RNA, Plant / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012231:Rhizobium; D016415:Sequence Alignment; D014158:Transcription, Genetic
D018749:RNA, Plant