[Proto-oncogene c-src regulates the viability of rat spermatogonial stem cells in vitro through phosphorylated signal transducer and activator of transcription-3].
The present study aimed to investigate the effect of proto-oncogene c-src on the viability of rat spermatogonial stem cells from 9 day-old rat in vitro. MTT method was used to observe the viability of the spermatogonial stem cells treated with antisense c-src oligodeoxynucleotides (ODNs) in vitro; RT-PCR was utilized to observe the expression of c-src mRNA and Western blot was used to observe the protein expressions of pp60c-src and phosphorylated signal transducer and activator of transcription-3 (p-STAT3). Compared with that in control group, the viability of spermatogonial stem cells decreased by 8.1% (P<0.05) and the expression of c-src mRNA decreased significantly after treatment with 10 μmol/L antisense c-src ODNs for 12 h. Compared with that in the control group, the protein expressions of pp60c-src and p-STAT3 decreased by 33.8% and 45.3% (both P<0.01), respectively, in the spermatogonial stem cells after being transfected with antisense c-src ODNs. The results suggest that proto-oncogene c-src regulates the viability of rat spermatogonial stem cells through p-STAT3.
Department of Physiology, Medical College of Nanchang University, Nanchang 330006, China.
Jia-Xiang
JX
Chen
Xin-Chang
XC
Wang
Jing-Lei
JL
Wang
Si-Fan
SF
Xu
Hai-Yan
HY
Qin
Bei
B
Yang
Jun-Ling
JL
Yang
Ting
T
Zou
D000818:Animals; D002478:Cells, Cultured; D016391:Genes, src; D008297:Male; D010766:Phosphorylation; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*; D012333:RNA, Messenger; D051381:Rats; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D014162:Transfection
D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; C494088:Stat3 protein, rat; D016392:Proto-Oncogene Proteins pp60(c-src)