Influence of dietary lipids on the erythrocyte antioxidant status of hypercholesterolaemic children.
The aim of this work was to examine the effect of dietary lipid intakes on the biomarkers of red cell antioxidant status in hypercholesterolaemic children. The study population included 34 children (18 boys and 16 girls) with cholesterol levels > or =5.2 mmol/l and 16 normolipidaemic children (9 boys and 7 girls) between 6 and 12 years of age. The status of the erythrocyte antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and reduced glutathione (GSH) were estimated spectrophotometrically. Dietary intake was assessed by 24-h recall and seven-day records. The hypercholesterolaemic children showed a decreased activity of antioxidant enzymes in relation to the control group. There was a negative correlation between energy intake and the activity of antioxidant enzymes (SOD and CAT) and GSH levels. Cholesterol intake was inversely correlated with CAT and GPx activity and GSH levels. The intake of polyunsaturated fat was positively correlated with the GPx activity. A decrease in the fat content of the diet for 6 months was proposed and 15 children followed the diet strictly. The activities of antioxidant enzymes in these children were significantly higher after the low-fat diet; the greatest increment was noted in the activity of GPx (91% with respect to the initial values), SOD was increased by 44% and CAT by 70%. We conclude that the intake of dietary lipids can modulate the antioxidant defence system, and an excess of energy and cholesterol has a negative influence on the antioxidant enzymes.
Department of Paediatrics, Obstetrics and Gynaecology, Faculty of Medicine and Odontology, University of Valencia, Avenida Blasco Ibáñez, 15, 46010, Valencia, Spain. pilar.codoner@uv.es
Pilar
P
Codoñer-Franch
Amalia
A
Bataller Alberola
José V
JV
Domingo Camarasa
María C
MC
Escribano Moya
Victoria
V
Valls Bellés
D000975:Antioxidants / Q000378:metabolism*; D015415:Biomarkers; D002374:Catalase / Q000097:blood / Q000187:drug effects; D002648:Child; D018752:Diet, Fat-Restricted; D004041:Dietary Fats / Q000494:pharmacology*; D004912:Erythrocytes / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005978:Glutathione / Q000097:blood / Q000187:drug effects; D005979:Glutathione Peroxidase / Q000097:blood / Q000187:drug effects; D005980:Glutathione Reductase / Q000097:blood / Q000187:drug effects; D006801:Humans; D006937:Hypercholesterolemia / Q000178:diet therapy / Q000378:metabolism*; D008297:Male; D013482:Superoxide Dismutase / Q000097:blood / Q000187:drug effects; D016896:Treatment Outcome
D000975:Antioxidants; D015415:Biomarkers; D004041:Dietary Fats; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005980:Glutathione Reductase; D005978:Glutathione