Effects of extracts from Panax notoginseng and Panax ginseng fruit on vascular endothelial cell proliferation and migration in vitro.
OBJECTIVE
To study the effects of extracts from Panax notoginseng (EPN) and Panax ginseng fruit (EPGF) on the proliferation and migration of human umbilical vein endothelial cells (HUVECs) in vitro.


METHODS
Cell proliferation was determined using an MTT method with a cultured HUVECs model cell cycle analyzed by cytometry. The effect on endothelial cell migration was investigated using an agarose scraping method. The content of vascular endothelial growth factor (VEGF) in the supernate was determined by enzyme-linked immunosorbent assay (ELISA). The VEGF mRNA expression of vascular endothelial cells (VECs) with different concentrations of EPN and EPGF was examined by reverse transcriptase-polymerase chain reaction (RT-PCR).


RESULTS
EPN and EPGF can promote the proliferation of VECs and the secretion of VEGF from HUVECs. It can increase the cell population significantly in the S phase to (15.22+/-1.33) % in the 50 mg/L dose group (P<0.05 or P<0.01). They can promote the VEC migration in the 200 mg/L dose group and the migration rate was 93.75% (P<0.01). They could also increase VEGF mRNA expression in VEC and the effects in the 100 mg/L and 50 mg/L dose groups were significant with the proportion of VEGF mRNA expression of 0.1812+/-0.0413 and 0.2037+/-0.0399 respectively (P<0.01).


CONCLUSIONS
EPN and EPGF can promote VEC proliferation, migration, DNA synthesis and VEGF mRNA expression. The results suggest that they have a certain effect on the genesis and development of new vessels in the ischemic myocardium.
Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, 100091, China. leiy999@163.com
Yan
Y
Lei
Qian
Q
Gao
Ke-ji
KJ
Chen
D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D042783:Endothelial Cells / Q000187:drug effects* / Q000502:physiology; D005638:Fruit; D006801:Humans; D005894:Panax; D010936:Plant Extracts / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
D004365:Drugs, Chinese Herbal; C519374:Panax notoginseng extract; D010936:Plant Extracts; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A