Influence of interferon alpha on expression of Fas and Fas ligand in dendritic cells from patients with chronic myeloid leukemia.
The study was aimed to investigate the influence of interferon alpha (IFN-alpha) on the expressions of Fas and Fas ligand (FasL) in dendritic cells (DCs) from patients with chronic myeloid leukemia (CML). In addition to adding stem cell factor (SCF), granulocyte-macrophage colony-stimulating factor (GM-CSF), tumor necrosis factor alpha (TNF-alpha) and interleukin 4 (IL-4), the IFN-alpha was added to the serum-free medium for DCs. After culturing for 10 - 14 days, cell phenotype and percentage of Ph(1) chromosome were detected by different methods. The expression of Fas or FasL on CML-DCs and cell cycle of DCs labeled with propidium iodine (PI) were measured by flow cytometry. The concentration of sFas in supernatants was analyzed by enzyme-linked immunosorbent assay (ELISA). The results indicated that the expression of co-stimulatory molecules were improved significantly while the percentages of Ph(1) positive cells decreased. The level of Fas on cells was up-regulated and the concentration of sFas decreased. However, the expression of FasL was negative. The ratio of apoptosis rose gradually while the concentration of IFN-alpha increased. It is concluded that IFN-alpha can accelerate the apoptosis of Ph(1) positive cells through Fas/FasL pathway, so the number of Ph(1) negative cells increases relatively.
Department of Hematology, The Affiliated Children Hospital of Suzhow University, Suzhou 215003, Jiangsn Province, China. zhaowenli69@yahoo.com.cn
Wen-Li
WL
Zhao
Yi-Huan
YH
Chai
Hai-Long
HL
He
Xu-Cang
XC
Wei
Tong
T
Wang
Pei-Ni
PN
Xing
Mei-Sheng
MS
Li
D000293:Adolescent; D000328:Adult; D017209:Apoptosis / Q000187:drug effects; D002478:Cells, Cultured; D002648:Child; D003470:Culture Media / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology / Q000378:metabolism*; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D016898:Interferon-alpha / Q000494:pharmacology*; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010677:Philadelphia Chromosome; D055815:Young Adult; D019014:fas Receptor / Q000235:genetics / Q000378:metabolism*
D003470:Culture Media; D053222:Fas Ligand Protein; D016898:Interferon-alpha; D019014:fas Receptor