Xanthine oxidase-dependent ROS production mediates vitamin A pro-oxidant effects in cultured Sertoli cells.
Several studies have suggested that vitamin A (retinol, ROH) presents pro-oxidant properties in biological systems. Recent studies point out that xantine oxidase, a ROS-generating enzyme, catalyses ROH oxidation to RA in vitro. These works stimulated the authors to investigate whether xanthine oxidase could be involved on the ROH pro-oxidative effects reported in cultured Sertoli cells. In vitro, it was demonstrate that xanthine oxidase generates superoxide in the presence of ROH as assessed by superoxide mediated-NBT reduction. Superoxide production is potentiated in the presence of NADH and inhibited by allopurinol. In Sertoli cells, ROH treatment increased xanthine oxidase activity and inhibition of the enzyme with allopurinol attenuated ROH-induced ROS production, protein damage and cytotoxicity. Moreover, inhibition of ROH oxidation to RA by retinaldehyde dehydrogenase inhibitor potentiated both xanthine oxidase-dependent ROS production and cell damage in ROH-treated cells. The data show that xanthine oxidase may play a role on vitamin A pro-oxidant effects.
Centro de Estudos em Estresse Oxidativo, Departamento de Bioquimica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. ohalceu@yahoo.com.br
Alfeu
A
Zanotto-Filho
Rafael
R
Schröder
José Cláudio F
JC
Moreira
D000493:Allopurinol / Q000737:chemistry; D000818:Animals; D008297:Male; D008954:Models, Biological; D009243:NAD / Q000378:metabolism; D016877:Oxidants / Q000378:metabolism*; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012708:Sertoli Cells / Q000166:cytology* / Q000378:metabolism; D013737:Testis; D013997:Time Factors; D014527:Uric Acid / Q000737:chemistry; D014801:Vitamin A / Q000378:metabolism*; D014969:Xanthine Oxidase / Q000378:metabolism*
D016877:Oxidants; D017382:Reactive Oxygen Species; D009243:NAD; D014801:Vitamin A; D014527:Uric Acid; D000493:Allopurinol; D014969:Xanthine Oxidase