Expression and regulation of HTRA1 during chick and early mouse development.
HTRA1, a member of the high temperature requirement factor A family, is a secreted serine protease that can bind to and inactivate members of the transforming growth factor-beta (TGFbeta) family, modulate insulin-like growth factor signaling and stimulate long range fibroblast growth factor (FGF) signaling in Xenopus. In vertebrates, so far homologues from mouse, human, and Xenopus have been cloned and studied. Here we report the cloning of the chicken HTRA1 homologue from a screen for FGF8 inducible genes in chick facial mesenchyme. We characterize its expression pattern from gastrulation (Hamburger and Hamilton stage 4) to day 4 of development, and in forming inner organs and limbs. We show that chick HTRA1 has a dynamic expression pattern that differs significantly from the expression of its mouse homolog. We, furthermore, demonstrate that FGF signaling is necessary and sufficient for HTRA1 expression in chick facial and forelimb mesenchyme, but is not required for HTRA1 expression in HH11 embryos.
Ferrer-Vaquer
Anna
A
Developmental Biology Unit, Institute of Biology I, University of Freiburg, Freiburg, Germany.
Maurey
Pablo
P
Werzowa
Johannes
J
Firnberg
Nicole
N
Leibbrandt
Andreas
A
Neubüser
Annette
A
D000818:Animals; D002642:Chick Embryo; D051524:Fibroblast Growth Factor 8 / Q000494:pharmacology; D054262:Gastrulation / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D000074289:High-Temperature Requirement A Serine Peptidase 1; D017403:In Situ Hybridization; D008648:Mesoderm / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D051379:Mice; D011758:Pyrroles / Q000494:pharmacology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000235:genetics* / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics / Q000502:physiology
D011758:Pyrroles; C105686:SU 5402; D051524:Fibroblast Growth Factor 8; D000074289:High-Temperature Requirement A Serine Peptidase 1; C492073:HtrA1 protein, mouse; D012697:Serine Endopeptidases