Simple and robust image-based autofocusing for digital microscopy.
A simple image-based autofocusing scheme for digital microscopy is demonstrated that uses as few as two intermediate images to bring the sample into focus. The algorithm is adapted to a commercial inverted microscope and used to automate brightfield and fluorescence imaging of histopathology tissue sections.
Yazdanfar
Siavash
S
GE Global Research, One Research Circle, Niskayuna, N.Y. 12309, USA. siavash.yazdanfar@research.ge.com
Kenny
Kevin B
KB
Tasimi
Krenar
K
Corwin
Alex D
AD
Dixon
Elizabeth L
EL
Filkins
Robert J
RJ
D000465:Algorithms; D001185:Artificial Intelligence; D007089:Image Enhancement / Q000379:methods*; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D012815:Signal Processing, Computer-Assisted