Phytotoxicity of cadmium on protein, proline and antioxidant enzyme activities in growing Arachis hypogaea L. seedlings.
Phytotoxicity of cadmium on growing Arachis hypogaea L. seedlings was studied. Seeds were exposed to 25, 50, and 100 micromol/L CdCl2 concentrations, for a period of 10, 15, 20 and 25 d. The extent of damage to chlorophyll, protein, proline, nitrate and nitrite reductase, antioxidant enzyme activity in leaves and roots were evaluated after 10 d of cadmium stress. The higher concentration of cadmium (100 micromol/L) resulted (leaves and roots) total chlorophyll 91.01%, protein 79.51%, 83.61%, nitrate reductase 79.39%, 80.72% and nitrite reductase 77.07%, 75.88% activity decreased with increase in cadmium concentrations and exposure periods. Cadmium caused significant changes in the activity of antioxidative enzymes. Contrastingly Cd treated plant tissues showed an increase in proline 159.87%, 239.6%, gluthion reductase (GR) 337.72%, 306.14%, superoxide disumutase (SOD) 688.56%, 381.72%, ascorbate peroxidase (APX) 226.47%, 252.14%, peroxidase (POD) 72.19%, 60.29% and catalase (CAT) 228.96%, 214.74% as compared to control. Cadmium stress caused a significant increase in the rate of SOD activity in leaves and roots of plant species. Results show the crop A. hypogaea is highly sensitive even at very low cadmium concentrations.
Department of Environmental Sciences, Sri Venkateswara University, Tirupati, Andhra Pradesh-517 502, India.
N
N
Dinakar
P C
PC
Nagajyothi
S
S
Suresh
Y
Y
Udaykiran
T
T
Damodharam
D000975:Antioxidants / Q000378:metabolism*; D010367:Arachis / Q000187:drug effects* / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D011392:Proline / Q000378:metabolism*; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism; D012989:Soil Pollutants / Q000633:toxicity*
D000975:Antioxidants; D010940:Plant Proteins; D012989:Soil Pollutants; D002104:Cadmium; D002734:Chlorophyll; D002338:Carotenoids; D011392:Proline; D010088:Oxidoreductases