Simian virus 40 large T overcomes p300 repression of c-Myc.
We previously showed that in quiescent cells p300/CBP negatively regulates the cell cycle G1-S transition by keeping c-Myc in a repressed state and that adenovirus E1A induces c-Myc by binding to p300/CBP. Studies have shown that p300/CBP binding to simian virus 40 large T is indirect and mediated by p53. By using a series of large T mutants that fail to bind to various cellular proteins including p53 as well as cells where p300 is overexpressed or p53 is knocked down, we show that the association of large T with p300 contributes to the induction of c-Myc and the cell cycle. The induction of c-Myc by this mechanism is likely to be important in large T mediated cell cycle induction and cell transformation.
Singhal
Ghata
G
Microbiology and Immunology Department, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
Kadeppagari
Ravi Kumar
RK
Sankar
Natesan
N
Thimmapaya
Bayar
B
D000952:Antigens, Polyomavirus Transforming / Q000494:pharmacology*; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism; D002460:Cell Line; D050881:E1A-Associated p300 Protein / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics / Q000378:metabolism*; D013539:Simian virus 40 / Q000737:chemistry*; D014158:Transcription, Genetic / Q000187:drug effects* / Q000235:genetics
D000952:Antigens, Polyomavirus Transforming; D018797:Cell Cycle Proteins; D016271:Proto-Oncogene Proteins c-myc; D050881:E1A-Associated p300 Protein