Direct electrochemistry and electrocatalysis of horseradish peroxidase in alpha-zirconium phosphate nanosheet film.
Alpha-zirconium phosphate nanosheets (ZrPNS) derived via the delamination of layered alpha-zirconium phosphate (alpha-ZrP) have been proven to be efficient support matrixes for the immobilization of horseradish peroxidase (HRP). X-ray powder diffraction (XRD) results revealed that ZrPNS in HRP-ZrPNS film remained unorderly structured for the effect of HRP. Fourier transform infrared (FTIR) spectra results revealed that HRP remained the secondary structure in HRP-ZrPNS film. The direct electrochemistry of HRP was realized in HRP-ZrPNS film on a glassy carbon electrode (GCE), showing a pair of well-defined, nearly reversible cyclic voltammetry (CV) peaks for the HRP heme Fe(III)/Fe(II) redox couple. The average surface concentration (Gamma(*)) of electroactive HRP in HRP-ZrPNS film was estimated to be 1.35x10(-10) mol cm(-2), which indicated a high loading of enzyme molecules in HRP-ZrPNS film. Based on these, a third generation reagentless biosensor was constructed for the determination of hydrogen peroxide (H(2)O(2)). The response time of the biosensor was less than 3 s, and the linear response range of the biosensor for H(2)O(2) was from 1.3x10(-6) to 1.6x10(-2) M with a correlation coefficient of 0.9997.
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China.
Xiushuang
X
Yang
Xu
X
Chen
Lan
L
Yang
Wensheng
W
Yang
D015374:Biosensing Techniques / Q000379:methods* / Q000592:standards; D002384:Catalysis; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000032:analysis; D049329:Nanostructures / Q000737:chemistry*; D017433:Protein Structure, Secondary; D015040:Zirconium / Q000737:chemistry*
D004800:Enzymes, Immobilized; C027006:zirconium phosphate; D006861:Hydrogen Peroxide; D015040:Zirconium; D006735:Horseradish Peroxidase