Glutathione S-transferase P1 ILE105Val polymorphism in occupationally exposed bladder cancer cases.
The genotype glutathione S-transferase P1 (GSTP1) influences the risk for bladder cancer among Chinese workers occupationally exposed to benzidine. Studies of Caucasian bladder cancer cases without known occupational exposures showed conflicting results. Research was thus conducted to define the role of GSTP1 genotypes in Caucasian bladder cancer cases with an occupational history of exposure to aromatic amines. DNA from 143 cases reported to the Industrial Professional Associations (Berufsgenossenschaften) in Germany from 1996 to 2004, who had contracted urothelial cancer due to occupational exposure, and 196 patients from one Department of Surgery in Dortmund, without known malignancy in their medical history, were genotyped using real-time polymerase chain reaction (PCR) (LightCycler) in relation to GSTP1 A1578G (Ile105Val) polymorphism. Among the subjects with bladder cancer, 46% presented the AA genotype, 39% the AG genotype, and 15% the GG genotype. In the surgical (noncancer) control group analyzed, 42% presented the AA genotype, 42% the AG genotype, and 16% the GG genotype. A subgroup of bladder cancer cases, represented by 46 painters, showed a distribution of 41% of the AA genotype, 48% of the AG genotype, and 11% of the GG genotype. Data indicated that in Caucasians exposed to aromatic amines the GSTP1 A1578G polymorphism did not appear to play a significant role as a predisposing factor for bladder cancer incidence.
Leibniz Research Centre for Working Environment and Human Factors, Institute for Occupational Physiology at the University of Dortmund (IfADo), Dortmund, Germany.
Silke
S
Kopps
Miriam
M
Angeli-Greaves
Meinolf
M
Blaszkewicz
Hans-Martin
HM
Prager
Hermann C
HC
Roemer
Dietrich
D
Lohlein
Wobbeke
W
Weistenhofer
Hermann M
HM
Bolt
Klaus
K
Golka
D000588:Amines / Q000009:adverse effects / Q000737:chemistry; D016022:Case-Control Studies; D004396:Coloring Agents / Q000009:adverse effects; D004785:Environmental Pollutants / Q000009:adverse effects; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D051549:Glutathione S-Transferase pi / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D016273:Occupational Exposure / Q000009:adverse effects*; D016017:Odds Ratio; D011084:Polycyclic Aromatic Hydrocarbons / Q000009:adverse effects; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced* / Q000453:epidemiology / Q000235:genetics*
D000588:Amines; D004396:Coloring Agents; D004785:Environmental Pollutants; D011084:Polycyclic Aromatic Hydrocarbons; C496556:GSTP1 protein, human; D051549:Glutathione S-Transferase pi