Metabolomics approach for determining growth-specific metabolites based on Fourier transform ion cyclotron resonance mass spectrometry.
Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR/MS) is the best MS technology for obtaining exact mass measurements owing to its great resolution and accuracy, and several outstanding FT-ICR/MS-based metabolomics approaches have been reported. A reliable annotation scheme is needed to deal with direct-infusion FT-ICR/MS metabolic profiling. Correlation analyses can help us not only uncover relations between the ions but also annotate the ions originated from identical metabolites (metabolite derivative ions). In the present study, we propose a procedure for metabolite annotation on direct-infusion FT-ICR/MS by taking into consideration the classification of metabolite-derived ions using correlation analyses. Integrated analysis based on information of isotope relations, fragmentation patterns by MS/MS analysis, co-occurring metabolites, and database searches (KNApSAcK and KEGG) can make it possible to annotate ions as metabolites and estimate cellular conditions based on metabolite composition. A total of 220 detected ions were classified into 174 metabolite derivative groups and 72 ions were assigned to candidate metabolites in the present work. Finally, metabolic profiling has been able to distinguish between the growth stages with the aid of PCA. The constructed model using PLS regression for OD(600) values as a function of metabolic profiles is very useful for identifying to what degree the ions contribute to the growth stages. Ten phospholipids which largely influence the constructed model are highly abundant in the cells. Our analyses reveal that global modification of those phospholipids occurs as E. coli enters the stationary phase. Thus, the integrated approach involving correlation analyses, metabolic profiling, and database searching is efficient for high-throughput metabolomics.
Takahashi
Hiroki
H
Graduate School of Information Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara, 630-0192, Japan.
Kai
Kosuke
K
Shinbo
Yoko
Y
Tanaka
Kenichi
K
Ohta
Daisaku
D
Oshima
Taku
T
Altaf-Ul-Amin
Md
M
Kurokawa
Ken
K
Ogasawara
Naotake
N
Kanaya
Shigehiko
S
D001431:Bacteriological Techniques; D048168:Escherichia coli K12 / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D005583:Fourier Analysis; D007477:Ions / Q000145:classification; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D008660:Metabolism; D015394:Molecular Structure; D010715:Phosphatidylglycerols / Q000032:analysis / Q000378:metabolism; D010743:Phospholipids / Q000032:analysis / Q000378:metabolism*
D007477:Ions; D010715:Phosphatidylglycerols; D010743:Phospholipids