An ultrafiltration membrane reactor for obtaining experimental reaction rates at defined concentrations of inhibiting sugars during enzymatic saccharification of alkali-pretreated sallow: Formulation of a simple empirical rate equation.
The kinetics of the enzymatic hydrolysis of sodium hydroxide-pretreated sallow were studied in an ultrafiltration membrane reactor in the presence of different concentrations of glucose. In the UF membrane reactor low-molecular-weight products were continuously removed at a low dilution rate and replaced by a buffer solution that contained different concentrations of glucose, which made it possible to keep the inhibiting product concentration constant throughout an experiment. The reaction rate was related to the degree of substrate conversion and a mathematical relationship was formulated that describes the influence of the product concentration on the rate coefficient.
Department of Food Engineering, University of Lund, Box 50, S-230 53 Alnarp, Sweden.
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Trägårdh
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Hahn-Hägerdal