Assay of the high-alkaline proteinase from alkalophilic bacillus PB92 using a chromogenic tripeptide substrate.
The chromogenic tripeptide substrate, benzyloxycarbonylglycyl-L-prolyl-L-citrulline p-nitroanilide, is proposed for the assay of the high-alkaline proteinase, HAP-PB92, from an alkalophilic Bacillus. The assay method is sensitive, reproducible, and may be adapted for an automatic system.
Department of Chemistry, University of Birmingham, P.O. Box 363 Birmingham, B15 2TT, United Kingdom.
C J
CJ
Gray
S A
SA
Barker
M S
MS
Dhariwal
J M
JM
Sullivan