MTA-induced neutrophil recruitment: a mechanism dependent on IL-1beta, MIP-2, and LTB4.
OBJECTIVE
The objective of this study was to investigate the mediators and the resident peritoneal cells involved in the neutrophil migration (NM) induced by mineral trioxide aggregate (MTA) in mice.


METHODS
MTA (25 mg/cavity) was injected into normal and pretreated peritoneal cavities (PC) with indomethacin (IND), dexamethasone (DEX), BWA4C, U75302, antimacrophage inflammatory protein-2 (MIP-2), and anti-interleukin-1beta (IL-1beta) antibodies and the NM was determined. The role of macrophage (MO) and mast cells (MAST) was determined by administration of thioglycollate 3% or 48/80 compound, respectively. The concentration of IL-1beta and MIP-2 exudates was measured by ELISA.


RESULTS
MTA induced dose- and time-dependent NM into mice PC, with the participation of MO and MAST. NM was inhibited by DEX, BWA4C, and U75302, as well as anti-MIP-2 and anti-IL-1beta antibodies. In the exudates, IL-1beta and MIP-2 were detected.


CONCLUSIONS
This study suggests that MTA induces NM via a mechanism dependent on MAST and MO mediated by IL-1beta, MIP-2, and LTB(4).
Department of Basic Sciences, Araçatuba Dentistry School, São Paulo State University, Araçatuba, SP, Brazil.
Alessandra Cristina
AC
Gomes
João Eduardo Gomes
JE
Filho
Sandra Helena Penha
SH
de Oliveira
D017607:Aluminum Compounds / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D017610:Calcium Compounds / Q000494:pharmacology*; D054426:Chemokine CXCL2 / Q000502:physiology*; D004338:Drug Combinations; D053583:Interleukin-1beta / Q000502:physiology*; D007975:Leukotriene B4 / Q000502:physiology*; D008264:Macrophages / Q000502:physiology; D008407:Mast Cells / Q000502:physiology; D051379:Mice; D008807:Mice, Inbred BALB C; D020556:Neutrophil Infiltration / Q000187:drug effects*; D010087:Oxides / Q000494:pharmacology*; D010529:Peritoneal Cavity / Q000166:cytology; D012387:Root Canal Filling Materials / Q000494:pharmacology*; D017640:Silicates / Q000494:pharmacology*
D017607:Aluminum Compounds; D000893:Anti-Inflammatory Agents; D017610:Calcium Compounds; D054426:Chemokine CXCL2; D004338:Drug Combinations; D053583:Interleukin-1beta; D010087:Oxides; D012387:Root Canal Filling Materials; D017640:Silicates; C086631:mineral trioxide aggregate; D007975:Leukotriene B4