Regulation of leptin, adiponectin and acylation-stimulating protein by hyperinsulinaemia and hyperglycaemia in vivo in healthy lean young men.
OBJECTIVE
Both type 1 and 2 diabetes are associated with differential regulation of leptin, adiponectin and ASP. Our aim was to examine whether or not acute hyperinsulinaemia and/or hyperglycaemia per se have differential regulation of these hormones in healthy subjects.


METHODS
We examined changes in leptin, adiponectin and ASP concentrations and subcutaneous white adipose tissue mRNA expression with 3-hour hyperinsulinaemic (HI, n=10), hyperglycaemic (HG, n=7) and hyperinsulinaemic-hyperglycaemic (HGHI, n=8) clamps in healthy lean young men. As somatostatin was used for the HG and HGHI clamps, a control somatostatin clamp was carried out (n=4). Changes in the expression of HKII and p85alpha Pi3K were examined as positive controls for the induction of gene expression by the insulin pathway.


RESULTS
HI, HG and HGHI clamps increased expression of HKII and p85alpha Pi3K while somatostatin did not. The HI clamp decreased serum adiponectin (-15%, P<0.001) and increased serum leptin (+11%, P=0.031), while the HG clamp reduced serum leptin (-20%, P=0.003). The HGHI clamp increased serum ASP (+21%, P=0.047) and expression of C3 (+26%, P=0.018) and leptin (+50%, P=0.024). Interestingly, the control somatostatin clamp suppressed both serum leptin (-17%, P=0.043) and adiponectin (-7%, P=0.020).


CONCLUSIONS
HG and/or HI per se regulated the concentrations and expression of leptin, adiponectin and ASP in healthy lean young men, suggesting a contribution to dysregulation of these hormones in diabetes.
Faraj
M
M
Unité métabolique, département de nutrition, faculté de médecine, université de Montréal, 2405, chemin Côte-Ste-Catherine, Montréal, Québec, H3T 1A8, Canada.
Beauregard
G
G
Tardif
A
A
Loizon
E
E
Godbout
A
A
Cianflone
K
K
Vidal
H
H
Rabasa-Lhoret
R
R
D052242:Adiponectin / Q000097:blood / Q000378:metabolism; D052436:Adipose Tissue, White / Q000378:metabolism; D000328:Adult; D001786:Blood Glucose / Q000378:metabolism*; D003176:Complement C3 / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism; D015309:Glucose Clamp Technique; D006801:Humans; D006943:Hyperglycemia / Q000378:metabolism*; D006946:Hyperinsulinism / Q000378:metabolism*; D007328:Insulin / Q000008:administration & dosage / Q000097:blood*; D020738:Leptin / Q000097:blood / Q000378:metabolism*; D008297:Male; D013004:Somatostatin / Q000008:administration & dosage
C499918:ADIPOQ protein, human; D052242:Adiponectin; D001786:Blood Glucose; C038245:C3 protein, human; D003176:Complement C3; D007328:Insulin; D020738:Leptin; D013004:Somatostatin