Apoptosis-inducing galactolipids from a cultured marine diatom, Phaeodactylum tricornutum.
Two monogalactosyl diacylglycerols, 1 and 2, were isolated from the marine diatom Phaeodactylum tricornutum, using the patented ApopScreen cell-based screen for apoptosis-inducing, potential anticancer compounds. The molecular structures of the galactolipids were determined using a combination of NMR, mass spectrometry, and chemical degradation. The bioactivities were confirmed using a specific apoptosis induction assay based on genetically engineered mammalian cell lines with differential, defined capacities for apoptosis. The galactolipids induce apoptosis in micromolar concentrations. This is the first report of apoptosis induction by galactolipids.
Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, New Brunswick, New Jersey 08901-8521, USA.
Eric H
EH
Andrianasolo
Liti
L
Haramaty
Assaf
A
Vardi
Eileen
E
White
Richard
R
Lutz
Paul
P
Falkowski
D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D017377:Diatoms / Q000737:chemistry*; D004354:Drug Screening Assays, Antitumor; D038983:Galactolipids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005818:Genetic Engineering; D008322:Mammals; D008386:Marine Biology; D015394:Molecular Structure
D000970:Antineoplastic Agents; D038983:Galactolipids; C009909:monogalactosyldiacylglycerol