Distinct actions of strontium on mineral formation in matrix vesicles.
Matrix vesicles (MVs) are involved in the initial step of mineralization in skeletal tissues and provide an easily model to analyze the hydroxyapatite (HA) formation. Sr stimulates bone formation and its effect was tested on MVs. Sr(2+) (15-50 microM) in the mineralization medium containing MVs, 2 mM Ca(2+) and 3.42 mM P(i), retarded HA formation. Sr(2+) (1-5 mM) in the same medium-induced other types of mineral than HA and cancelled the ATP-, ADP- or PP(i)-induced retardation in the mineral formation. Our findings suggest that the beneficial effect of Sr(2+) at a low dose (15-50 microM) is rather an inhibitor of bone resorption than an activator of mineral formation, while at high Sr(2+) concentration (1-5 mM), mineral formation, especially other types of mineral than HA, is favored.
Bechkoff
Géraldine
G
Université de Lyon, Villeurbanne F-69622, France.
Radisson
Jacqueline
J
Bessueille
Laurence
L
Bouchekioua-Bouzaghou
Katia
K
Buchet
René
R
D000255:Adenosine Triphosphate / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D001862:Bone Resorption / Q000378:metabolism; D002113:Calcification, Physiologic / Q000187:drug effects*; D002413:Cations, Divalent / Q000494:pharmacology; D002642:Chick Embryo; D017886:Durapatite / Q000378:metabolism*; D005109:Extracellular Matrix / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis / Q000187:drug effects*; D022142:Secretory Vesicles / Q000187:drug effects* / Q000378:metabolism; D013055:Spectrophotometry, Infrared; D013324:Strontium / Q000494:pharmacology*
D002413:Cations, Divalent; D000255:Adenosine Triphosphate; D017886:Durapatite; D013324:Strontium