Search for potential markers for prostate cancer diagnosis, prognosis and treatment in clinical tissue specimens using amine-specific isobaric tagging (iTRAQ) with two-dimensional liquid chromatography and tandem mass spectrometry.
This study aimed to identify candidate new diagnosis and prognosis markers and medicinal targets of prostate cancer (PCa), using state of the art proteomics. A total of 20 prostate tissue specimens from 10 patients with benign prostatic hyperplasia (BPH) and 10 with PCa (Tumour Node Metastasis [TNM] stage T1-T3) were analyzed by isobaric stable isotope labeling (iTRAQ) and two-dimensional liquid chromatography-tandem mass spectrometry (2DLC-MS/MS) approaches using a hybrid quadrupole time-of-flight system (QqTOF). The study resulted in the reproducible identification of 825 nonredundant gene products (p < or = 0.05) of which 30 exhibited up-regulation (> or =2-fold) and another 35 exhibited down-regulation (< or =0.5-fold) between the BPH and PCa specimens constituting a major contribution toward their global proteomic assessment. Selected findings were confirmed by immunohistochemical analysis of prostate tissue specimens. The proteins determined support existing knowledge and uncover novel and promising PCa biomarkers. The PCa proteome found can serve as a useful aid for the identification of improved diagnostic and prognostic markers and ultimately novel chemopreventive and therapeutic targets.
Garbis
Spiros D
SD
Biomedical Research Foundation, Academy of Athens, Greece. sgarbis@bioacademy.gr
Tyritzis
Stavros I
SI
Roumeliotis
Theodoros
T
Zerefos
Panagiotis
P
Giannopoulou
Eugenia G
EG
Vlahou
Antonia
A
Kossida
Sophia
S
Diaz
Jose
J
Vourekas
Stavros
S
Tamvakopoulos
Constantin
C
Pavlakis
Kitty
K
Sanoudou
Despina
D
Constantinides
Constantinos A
CA
D000368:Aged; D000595:Amino Acid Sequence; D015415:Biomarkers / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D002853:Chromatography, Liquid; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008875:Middle Aged; D008969:Molecular Sequence Data; D011379:Prognosis; D017430:Prostate-Specific Antigen / Q000378:metabolism; D011470:Prostatic Hyperplasia / Q000175:diagnosis / Q000378:metabolism* / Q000628:therapy; D011471:Prostatic Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000628:therapy; D020543:Proteome / Q000378:metabolism*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
D015415:Biomarkers; D014408:Biomarkers, Tumor; D020543:Proteome; D017430:Prostate-Specific Antigen