The role of antibodies specific for toxic sPLA2s and haemorrhagins in neutralizing potential of antisera raised against Vipera ammodytes ammodytes venom.
The contribution of antibodies directed against the two main toxic groups of proteins in the Vipera ammodytes ammodytes venom, haemorrhagic metalloproteinases (H) and neurotoxic sPLA2s (Atxs), to the overall protective efficacy of the whole venom antisera was investigated. Using ELISA assays we established a high correlation between the protective efficacy of the whole venom antisera in mice and their anti-Atxs antibody content. As the haemorrhage is the prevailing toxic effect of the venom in human, the lack of correlation also with anti-H IgG content exposed that the mouse model might not be optimal to evaluate the neutralizing potential of the venom-specific antisera for human therapy. We further revealed that Atxs and structurally very similar but non-toxic AtnI2 from the venom are not immuno cross-reactive.
Halassy
Beata
B
Research and Development Department, Institute of Immunology, Inc., Rockefellerova 10, HR-10 000 Zagreb, Croatia. bhalassy@imz.hr
Habjanec
Lidija
L
Brgles
Marija
M
Balija
Maja Lang
ML
Leonardi
Adrijana
A
Kovacic
Lidija
L
Prijatelj
Petra
P
Tomasić
Jelka
J
Krizaj
Igor
I
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D000997:Antivenins / Q000276:immunology / Q000494:pharmacology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D054501:Group II Phospholipases A2 / Q000276:immunology; D007106:Immune Sera / Q000276:immunology / Q000494:pharmacology*; D007114:Immunization; D007928:Lethal Dose 50; D045726:Metalloproteases / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D051379:Mice; D009500:Neutralization Tests; D054497:Phospholipases A2, Secretory / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D011817:Rabbits; D014757:Viper Venoms / Q000037:antagonists & inhibitors* / Q000201:enzymology / Q000276:immunology / Q000633:toxicity; D017819:Viperidae / Q000276:immunology*
D000941:Antigens; D000997:Antivenins; D007106:Immune Sera; D014757:Viper Venoms; C068117:ammodytoxin A; D054501:Group II Phospholipases A2; D054497:Phospholipases A2, Secretory; C060161:ammodytoxin C; D045726:Metalloproteases