Use of a recycle-type SEC method as a powerful tool for purification of thiosialoside derivatives.
An efficient separation between fully acetylated thiosialoside methyl esters and fully acetylated Neu5Ac2en methyl esters was accomplished by means of a size-exclusion chromatography (SEC) method. Purity determinations and structural elucidation of the isolated compounds were performed by a combination of elemental analyses and spectroscopic analyses, including IR, (1)H, and (13)C NMR, and mass spectroscopic analyses.
Sakamoto
Jun-Ichi
J
Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
Takita
Chiharu
C
Koyama
Tetsuo
T
Hatano
Ken
K
Terunuma
Daiyo
D
Matsuoka
Koji
K
D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D054330:Thiosugars / Q000737:chemistry / Q000302:isolation & purification*
D054330:Thiosugars