Effect of resin adhesive systems on root caries formation in vitro.
OBJECTIVE
To evaluate the capability of different adhesive systems to inhibit in vitro caries formation.


METHODS
Four materials were tested: Gluma Comfort Bond (GL; Heraeus Kulzer), Gluma Comfort Bond + Desensitizer (GL+; Heraeus Kulzer), iBond (iB; Heraeus Kulzer), and One-up Bond F (OUB; Tokuyama). Bovine roots were ground to obtain flat mesial and distal dentin surfaces. Nail varnish was applied to the entire root surface except for two 10 mm X 2 mm windows on the flattened surfaces. The adhesives were applied to the exposed areas according to the manufacturers' instructions. The roots were incubated at 37 degrees C for 1 week in a suspension of StreptococcuS mutans and Lactobacillus acidophilus. Control groups either received no root surface treatment or were etched with 37% phosphoric acid (without immersion in the bacterial suspension). Specimens were sectioned perpendicular to the long axis of the roots, stained for 24 hours with Rhodamine B, and analyzed with confocal laser microscopy. The data were analyzed by 1-way ANOVA and Fisher's PLSD test at 95% confidence level.


RESULTS
Lesions in the OUB, iB, and GL+ groups were significantly shallower than those in the no-treatment group. Acid etching did not produce any measurable lesions. Mean lesion depth in the GL group was similar to that in the no-treatment group. There was no significant difference between mean lesion depths in the GL+, GL, and iB groups.


CONCLUSIONS
Fluoride- and glutaraldehyde-containing adhesive systems might be an aid in root caries prevention.
Department of Operative Dentistry, The University of North Carolina at Chapel Hill, NC 27599-7450, USA. rick_walter@dentistry.unc.edu
Ricardo
R
Walter
Wagner R
WR
Duarte
Patricia N R
PN
Pereira
Edward J
EJ
Swift
Harold O
HO
Heymann
Roland R
RR
Arnold
D000134:Acid Etching, Dental; D000818:Animals; D002417:Cattle; D017220:Dentin-Bonding Agents / Q000737:chemistry*; D005456:Fluorescent Dyes; D007779:Lactobacillus acidophilus / Q000502:physiology; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D018613:Microscopy, Confocal; D010756:Phosphoric Acids / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry*; D012235:Rhodamines; D017213:Root Caries / Q000382:microbiology* / Q000473:pathology; D013295:Streptococcus mutans / Q000502:physiology; D013696:Temperature; D013997:Time Factors
D017220:Dentin-Bonding Agents; D005456:Fluorescent Dyes; C445021:Gluma Comfort Bond; C445022:Gluma Comfort Bond & Desensitizer; D008689:Methacrylates; C446260:One-Up-Bond F; D010756:Phosphoric Acids; D019279:Resin Cements; D012235:Rhodamines; C477386:iBond; C030242:phosphoric acid; C029773:rhodamine B