VAMP2 is expressed in myogenic cells during rat development.
Vesicle-associated membrane protein 2 (VAMP2) is a member of the SNARE family of proteins that regulate the intracellular vesicle fusion process. This study investigated the developmental expression of VAMP2 in the rat embryo. In the trunk, VAMP2 was primarily found in the heart on embryonic day (E) 10. On E12.5, VAMP2 expression was found in nerve fibers, somites, and heart. In somites, epithelial cells in the dorsomedial lip, and elongated myoblasts in myotome were positive for VAMP2. On E16.5, VAMP2 was expressed in the heart, nerve fibers, and skeletal muscles. In skeletal muscles, multinuclear myotubes were positive for VAMP2. In the head, where muscles are derived both from somitic and non-somitic origin, VAMP2 was found in myotubes of the extrinsic ocular muscles and masseter muscle on E16.5. These findings suggest the involvement of VAMP2 in the development of skeletal muscles of somitic and non-somitic origins.
Department of Anatomy, Gunma University Graduate School of Medicine, Gunma, Japan.
Yuki
Y
Tajika
Tohru
T
Murakami
Mahito
M
Sato
Fumitaka
F
Kubota
Hiroshi
H
Yorifuji
D000818:Animals; D005260:Female; D005455:Fluorescent Antibody Technique; D006321:Heart / Q000196:embryology; D018613:Microscopy, Confocal; D024510:Muscle Development; D018485:Muscle Fibers, Skeletal / Q000166:cytology / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D032446:Myoblasts / Q000166:cytology / Q000378:metabolism; D009206:Myocardium / Q000166:cytology / Q000378:metabolism; D009412:Nerve Fibers / Q000378:metabolism; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar; D019170:Somites / Q000166:cytology / Q000196:embryology / Q000378:metabolism; D050988:Vesicle-Associated Membrane Protein 2 / Q000378:metabolism*
D050988:Vesicle-Associated Membrane Protein 2