Immunolocalization of deformed wing virus particles within the mite Varroa destructor.
Deformed wing virus (DWV) induces wing deformation when bees are infected during their pupal development. Field observations and laboratory experiments suggest that the mite Varroa destructor is a vector of the virus. Moreover, it has been stated that DWV replicates within this mite. In order to understand the role of V. destructor in the transmission of DWV, the objective of this work was to locate the sites of retention and/or replication of DWV within the mite by immunohistochemistry. There was no evidence that DWV was replicating in the mite as no tissues showed specific antibody binding to DWV. Also, there were no specific structures that could be suggested as retention sites. DWV was found only in the midgut lumen of V. destructor in structures resembling large, dense spheres, which were presumably faecal pellets.
Santillán-Galicia
Ma Teresa
MT
School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK.|Centre for Soils and Ecosystem Function, Department of Plant and Invertebrate Ecology, Rothamsted Research, Harpenden AL5 2JQ, UK.
Carzaniga
Raffaella
R
Centre for Bioimaging, Department of Plant Pathology and Microbiology, Rothamsted Research, Harpenden AL5 2JQ, UK.
Ball
Brenda V
BV
Centre for Soils and Ecosystem Function, Department of Plant and Invertebrate Ecology, Rothamsted Research, Harpenden AL5 2JQ, UK.
Alderson
Peter G
PG
School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK.
D000818:Animals; D005243:Feces / Q000821:virology; D041981:Gastrointestinal Tract / Q000821:virology; D007150:Immunohistochemistry; D008925:Mites / Q000821:virology*; D012328:RNA Viruses / Q000737:chemistry* / Q000276:immunology; D014764:Viral Proteins / Q000032:analysis* / Q000276:immunology; D014771:Virion / Q000737:chemistry* / Q000276:immunology
D014764:Viral Proteins