Biosurfactant production by Rhodococcus erythropolis grown on glycerol as sole carbon source.
The production of biosurfactant by Rhodococcus erythropolis during the growth on glycerol was investigated. The process was carried out at 28 degrees C in a 1.5-L bioreactor using glycerol as carbon source. The bioprocess was monitored through measurements of biosurfactant concentration and glycerol consumption. After 51 h of cultivation, 1.7 g/L of biosurfactant, surface, and interfacial tensions values (with n-hexadecane) of 43 and 15 mN/m, respectively, 67% of Emulsifying Index (E (24)), and 94% of oil removal were obtained. The use of glycerol rather than what happens with hydrophobic carbon source allowed the release of the biosurfactant, originally associated to the cell wall.
Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Centro de Tecnologia, Bloco E, CEP 21949-900, Rio de Janeiro, RJ, Brasil.
Elisa M P
EM
Ciapina
Walber C
WC
Melo
Lidia M M
LM
Santa Anna
Alexandre S
AS
Santos
Denise M G
DM
Freire
Nei
N
Pereira
D019149:Bioreactors / Q000382:microbiology*; D002244:Carbon / Q000378:metabolism*; D018929:Cell Culture Techniques / Q000379:methods*; D005990:Glycerol / Q000378:metabolism*; D012240:Rhodococcus / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D013501:Surface-Active Agents / Q000032:analysis / Q000737:chemistry* / Q000378:metabolism*
D012722:Sewage; D013501:Surface-Active Agents; D002244:Carbon; D005990:Glycerol