Oxidative stress in primary open-angle glaucoma.
OBJECTIVE
To analyze oxidative stress in primary open-angle glaucoma (POAG).


METHODS
A case-control study including 90 eyes of 90 patients who needed antiglaucomatous surgery in the course of POAG (glaucoma group, n=50) and from patients who were operated of nonpathologic cataracts (cataract group, n=40). Free radical formation via lipid peroxidation by malondialdehyde-thiobarbituric acid reactive substances (MDA-TBARS) test and total antioxidant status in the aqueous humor samples of both groups were determined. Statistical analyses were carried out in relation to MDA-TBARS and total antioxidant status and their correlations with glaucoma risk factors.


RESULTS
Significantly higher MDA-TBARS were detected in the POAG with respect to the comparative group of cataract subjects (P<0.001). Antioxidant activity was significantly lower in the POAG than in the cataract group (P<0.001).


CONCLUSIONS
Aqueous humor samples may be used for determining oxidative and antioxidant status in pathologic processes. Glaucomatous eyes had a significant increase in oxidative status and decreased antioxidant activity in the aqueous humor than the cataract eyes. Oxidative stress may play a pathogenical role in the POAG.
Ophthalmologic Research Unit Santiago Grisolia, Dr Peset Universitary Hospital, Valencia, Spain. zanon_vicmor@gva.es
Vicente
V
Zanon-Moreno
Pilar
P
Marco-Ventura
Antonio
A
Lleo-Perez
Sheila
S
Pons-Vazquez
Jose J
JJ
Garcia-Medina
Ignacio
I
Vinuesa-Silva
Maria A
MA
Moreno-Nadal
Maria Dolores
MD
Pinazo-Duran
D000368:Aged; D000369:Aged, 80 and over; D001082:Aqueous Humor / Q000502:physiology; D016022:Case-Control Studies; D002386:Cataract / Q000503:physiopathology; D002908:Chronic Disease; D005260:Female; D005609:Free Radicals; D005902:Glaucoma, Open-Angle / Q000503:physiopathology*; D006801:Humans; D015227:Lipid Peroxidation; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008875:Middle Aged; D018384:Oxidative Stress / Q000502:physiology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D005609:Free Radicals; D017392:Thiobarbituric Acid Reactive Substances; D008315:Malondialdehyde