Induced hydrolytic enzymes of ectomycorrhizal fungi against pathogen Rhizoctonia solani.
Interactions between ectomycorrhizal fungi (Suillus laricinus, S. tomentosus, Amanita vaginata and Gomphidius viscidus) and the pathogen Rhizoctonia solani in co-culture were studied using both light and scanning electron microscopy. S. laricinus, S. tomentosus and A. vaginata inhibited the growth of the pathogen. Moreover, A. vaginata exhibited coiling around and penetration of the hyphae into R. solani was observed in the interaction zone. Furthermore, the production of chitinases, beta-1,3-glucanases and beta-glucosidases by these ectomycorrhizal fungi on colloidal chitin or cell walls of R. solani was evaluated: chitinases were not induced by colloidal chitin but all three enzymes were induced by R. solani cell walls. No correlation between inhibition rate and production of lytic enzymes was found.
College of Forestry, Northwest A & F University, Yangling, Shaanxi 712100, China. tangm@nwsuaf.edu.cn
Ming
M
Tang
Ru-Qin
RQ
Zhang
Hui
H
Chen
Hai-Han
HH
Zhang
Zhi-Qiang
ZQ
Tian
D044602:Cellulases / Q000096:biosynthesis* / Q000378:metabolism; D002688:Chitinases / Q000096:biosynthesis* / Q000378:metabolism; D018920:Coculture Techniques; D004790:Enzyme Induction; D043326:Glucan 1,3-beta-Glucosidase / Q000096:biosynthesis* / Q000378:metabolism; D006868:Hydrolysis; D038821:Mycorrhizae / Q000201:enzymology*; D012232:Rhizoctonia / Q000378:metabolism* / Q000472:pathogenicity
D044602:Cellulases; D002688:Chitinases; D043326:Glucan 1,3-beta-Glucosidase; C117648:beta-1,3-exoglucanase