A simple one-step real-time RT-PCR for diagnosis of dengue virus infection.
Dengue is the most important arbovirus disease in tropical and sub-tropical countries, and can be caused by infection with any of the four-dengue virus (DENV) serotypes. Infection with DENV can lead to a broad clinical spectrum, ranging from sub-clinical infection or an influenza-like disease known as dengue fever (DF) to a severe, sometimes fatal, disease characterized by hemorrhage and plasma leakage that can lead to shock, known as dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS). The diagnosis of dengue is routinely accomplished by serologic assays, such as IgM and IgG ELISAs, as well as HI tests, analyzing serum samples obtained from patients with at least 7 days of symptoms onset. These tests cannot be used for diagnosis during the early symptomatic phase. In addition, antibodies against dengue are broad reactive with other flaviviruses. Therefore, a specific diagnostic method for acute DENV infection is of great interest. In that sense, the real-time RT-PCR has become an important tool that can be used for early and specific detection of dengue virus genome in human serum samples. This study describes a simple, specific, and sensitive real-time RT-PCR for early diagnosis of dengue virus infection.
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas, USP, Ribeirão Preto, SP, Brazil.
Harryson Wings Godoy
HW
Dos Santos
Telma Regina Ramos Silva
TR
Poloni
Kelly Paula
KP
Souza
Vanessa Danielle Menjon
VD
Muller
Flávia
F
Tremeschin
Lívia Christensen
LC
Nali
Leandro Ricardo
LR
Fantinatti
Alberto Anastacio
AA
Amarilla
Helda Liz Alfonso
HL
Castro
Marcio Roberto
MR
Nunes
Samir Mansour
SM
Casseb
Pedro Fernando
PF
Vasconcelos
Soraya Jabur
SJ
Badra
Luiz Tadeu Moraes
LT
Figueiredo
Victor Hugo
VH
Aquino
D000293:Adolescent; D000328:Adult; D000368:Aged; D052160:Benzothiazoles; D002648:Child; D003715:Dengue / Q000175:diagnosis* / Q000821:virology; D003716:Dengue Virus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D003959:Diamines; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D008297:Male; D008875:Middle Aged; D009930:Organic Chemicals; D011804:Quinolines; D012367:RNA, Viral / Q000097:blood* / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D019595:Severe Dengue / Q000175:diagnosis / Q000821:virology
D052160:Benzothiazoles; D003959:Diamines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D009930:Organic Chemicals; D011804:Quinolines; D012367:RNA, Viral; C098022:SYBR Green I