Humoral immune response after genetic immunization is consistently improved by electroporation.
Aiming to evaluate some parameters to influence the immune response to DNA vaccination, we compare three protocols of DNA immunization (i.m. injections, i.m. injections followed by electroporation, and the effect of i.p. injection of stably antigen-transfected cells before DNA administration), using three different antigens. Statistical analyses showed that electroporation after intramuscular injections provided an immune response comparable to that obtained by pre-treatment with antigen-transfected cells and similar to that obtained by protein immunization. The results allowed us selecting a protocol that worked well for all three antigens and reinforced the idea that high level of gene expression is essential to get good immunization.
Biophysics Department, UNIFESP, Sao Paulo, SP, Brazil.
Carolina Bellini
CB
Parise
Bianca
B
Lisboa
Daniela
D
Takeshita
Chester Bittencourt
CB
Sacramento
Jane Zveiter
JZ
de Moraes
Sang Won
SW
Han
D019264:Adoptive Transfer; D000818:Animals; D018274:Electroporation; D005260:Female; D007273:Injections, Intramuscular; D051379:Mice; D008807:Mice, Inbred BALB C; D014611:Vaccination / Q000379:methods*; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology*
D019444:Vaccines, DNA