Combining chip-ESI with APLI (cESILI) as a multimode source for analysis of complex mixtures with ultrahigh-resolution mass spectrometry.
Recently we have established atmospheric-pressure laser ionisation (APLI) as a method for coupling time-of-flight mass spectrometric detectors (TOF MS) with chromatographic systems (HPLC and GC) to allow two-photon ionisation of non-polar aromatic compounds. Here we demonstrate that APLI can be combined with chip-electrospray ionisation (cESI) coupled to Fourier-transform-ion cyclotron resonance mass spectrometry (FT-ICR MS) for ultrahigh-resolution analysis of complex samples. With the laser turned off, the analytes are ionised only by ESI, whereas when the laser is switched on non-polar aromatic substances also are ionised. In combination with the extremely high mass resolution of an FT-ICR MS, simultaneous qualitative analysis of polar and non-polar analytes is possible in both positive and negative modes, as is exemplified with a crude oil sample. Nevertheless, ion suppression was observed (up to ca. 70% for D(10)-pyrene) and thus sample preparation with chromatographic or electrophoretic pre-separation is necessary for quantitative analysis of targets. In addition, for the first time, the dopant-assisted APLI method in combination with cESI (DA-cESILI) was used for determination of 1-nitrocoronene.
German Research Center for Environmental Health, Institute for Ecological Chemistry, Helmholtz Zentrum München, Ingoldstaedter Landstrasse 1, 85764, Neuherberg, Germany.
Philippe
P
Schmitt-Kopplin
Matthias
M
Englmann
Ramon
R
Rossello-Mora
Ralf
R
Schiewek
Klaus J
KJ
Brockmann
Thorsten
T
Benter
Oliver J
OJ
Schmitz
D000097:Acetonitriles / Q000737:chemistry; D001274:Atmospheric Pressure; D001564:Benzo(a)pyrene / Q000032:analysis*; D005583:Fourier Analysis; D015394:Molecular Structure; D011083:Polycyclic Compounds / Q000032:analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods
D000097:Acetonitriles; D011083:Polycyclic Compounds; D001564:Benzo(a)pyrene; C040176:1-nitrocoronene; C032159:acetonitrile