Whole-genome transcriptional profiles of a novel marine fish iridovirus, Singapore grouper iridovirus (SGIV) in virus-infected grouper spleen cell cultures and in orange-spotted grouper, Epinephulus coioides.
A DNA microarray containing all Singapore grouper iridovirus (SGIV) open reading frames (ORFs) was constructed to map the viral gene transcriptional profiles in virus-infected grouper spleen (GS) cells and in spleen tissues of virus-infected grouper. The results showed that viral genes started to be transcribed as early as 1 h postinfection (p.i.), and followed by a rapid increasing gene expression along with virus infection in cell cultures. The three temporal kinetic classes (15 immediate-early, 89 early and 53 late transcripts) were classified during an in vitro infection by their dependence on de novo protein synthesis and viral DNA replication inhibitors. In SGIV-infected grouper, Epinephulus coioides, most of the viral genes were expressed between 1 and 4 d p.i., and the number and expression levels started to decrease after 5 d p.i. These data were confirmed by real-time RT-PCR. This study provides an experimental basis for investigation of virus-host interactions and the development of control strategies against SGIV infection.
State Key Laboratory of Biocontrol, College of Life Sciences, Sun Yat-sen (Zhongshan) University, 135 West Xingang Road, Guangzhou 510275, China.
Yong
Y
Teng
Zhiwei
Z
Hou
Jie
J
Gong
Hong
H
Liu
Xiayang
X
Xie
Liang
L
Zhang
Xiaohong
X
Chen
Qi Wei
QW
Qin
D000818:Animals; D001483:Base Sequence; D001492:Bass / Q000821:virology*; D002478:Cells, Cultured; D017931:DNA Primers / Q000235:genetics; D004266:DNA Virus Infections / Q000662:veterinary / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D005393:Fish Diseases / Q000821:virology; D020869:Gene Expression Profiling; D016679:Genome, Viral; D017994:Iridovirus / Q000235:genetics* / Q000472:pathogenicity*; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors
D017931:DNA Primers; D004279:DNA, Viral