Nanoscale devices for online dielectric spectroscopy of biological cells.
Nanoscale probes have been developed for the online characterization of the electrical properties of biological cells by dielectric spectroscopy. Two types of sensors have been designed and fabricated. The first one is devoted to low (<10 MHz) frequency range analysis and consists of gold nanoelectrodes. The second one works for high (>40 Hz) frequency range analysis and consists of a gold nanowire. The patterning of the sensors is performed by electron beam lithography. These devices are integrated in a microfluidic channel network for the manipulation of the cells and for the improvement of the performances of the sensors. These devices are used for the analysis of a well-characterized biological model in the area of the ligand-receptor interaction. The purpose is to monitor the interaction between the lactoferrin (the ligand) and the nucleolin and sulfated proteoglycans (the receptors) present or not on a set of mutant Chinese hamster ovary cell lines and their following internalization into the cytoplasm. Initial measurements have been performed with this microsystem and they demonstrate its capability for label-free, real-time, analysis of a dynamic mechanism involving biological cells.
Institut d'Electronique, de Microélectronique et de Nanotechnologie, IEMN/CNRS-USTL-ISEN, University of Lille, Villeneuve d'Ascq, France.
Damien
D
Debuisson
Anthony
A
Treizebré
Timothée
T
Houssin
Eric
E
Leclerc
Dominique
D
Bartès-Biesel
Dominique
D
Legrand
Joel
J
Mazurier
Steve
S
Arscott
Bertrand
B
Bocquet
Vincent
V
Senez
D000818:Animals; D016466:CHO Cells; D003198:Computer Simulation; D006224:Cricetinae; D003412:Cricetulus; D017097:Electric Impedance; D006046:Gold / Q000378:metabolism; D006801:Humans; D007781:Lactoferrin / Q000378:metabolism; D044085:Microfluidics; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D009862:Online Systems; D013057:Spectrum Analysis / Q000379:methods*
D006046:Gold; D007781:Lactoferrin