Immunity gene pedB enhances production of pediocin PA-1 in naturally resistant Lactococcus lactis strains.
Heterologous production of the antilisterial bacteriocin pediocin PA-1 in lactococci is an attractive objective to increase the safety of dairy products. In a previous paper, we developed a system for the heterologous production of the bacteriocin pediocin PA-1 in pediocin-resistant lactococcal hosts through a leader exchange strategy. The system was based on 3 genes, 1 encoding the fusion between the lactococcin A leader and propediocin PA-1, and the other 2 encoding the lactococcin A secretion machinery. In this study, we investigated whether the addition of the pediocin PA-1 immunity gene (pedB) to this system has any effect on pediocin production. Introduction of the plasmid(s) carrying the genes described above into nisinproducing and non-nisinproducing lactococcal hosts led to a significant increase in the production of pediocin compared with the equivalent pedB-devoid systems. In addition, we obtained a nisin-producing strain with the ability to secrete pediocin PA-1 at a level equivalent to that of the parental strain Pediococcus acidilactici 347, which represents a notable improvement over our previous systems.
Commensals and Microflora, BBSRC Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, United Kingdom. juan.arques@bbsrc.ac.uk
J L
JL
Arqués
J M
JM
Rodríguez
M J
MJ
Gasson
N
N
Horn
D000900:Anti-Bacterial Agents / Q000096:biosynthesis* / Q000276:immunology; D001430:Bacteriocins / Q000096:biosynthesis* / Q000235:genetics / Q000276:immunology; D003257:Consumer Product Safety; D003611:Dairy Products / Q000382:microbiology*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D013294:Lactococcus lactis / Q000235:genetics* / Q000378:metabolism*; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D009561:Nisin / Q000096:biosynthesis / Q000276:immunology; D000070017:Pediocins; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D000070017:Pediocins; D011993:Recombinant Fusion Proteins; C074288:pediocin PA-1; C069325:lactococcin A; D009561:Nisin