Voltammetric evaluation of the binding between wheat germ agglutinin and thionine/glucose-modified magnetic microbeads.
The binding between glucose residues and wheat germ agglutinin (WGA) on thionine/glucose-modified magnetic microbeads was evaluated using voltammetry. Thionine is an electroactive compound and has two amino groups. Thionine was immobilized to magnetic beads via cross-linking of the amino groups on the beads with an amino group on thionine. Glucose was bound to the other amino group of thionine via the formation of a Schiff base. The beads were only several micrometers in size the same size, as cells. WGA-binding to glucose on the bead surface blankets the thionine moiety. Thus, WGA-binding could be detected as a decrease in peak current of the thionine moiety.
Sugawara
Kazuharu
K
Faculty of Education, Gunma University, Maebashi, Gunma, Japan. kzsuga@edu.gunma-u.ac.jp
Senbongi
Atsushi
A
Kamiya
Naoto
N
Hirabayashi
George
G
Kuramitz
Hideki
H
D004563:Electrochemistry; D005947:Glucose / Q000378:metabolism*; D008280:Magnetics; D008863:Microspheres; D010640:Phenothiazines / Q000378:metabolism*; D011485:Protein Binding; D013997:Time Factors; D014909:Wheat Germ Agglutinins / Q000378:metabolism*
D010640:Phenothiazines; D014909:Wheat Germ Agglutinins; D005947:Glucose; C009469:thionine