Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development.
OBJECTIVE
To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development.


METHODS
Animal study.


METHODS
University laboratory.


METHODS
CD-1 mice.


METHODS
Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 micromol/L, 5 micromol/L, and 10 micromol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium.


METHODS
Oocyte maturation, fertilization, and early embryonic development in vitro.


RESULTS
After 6 and 24 hours of prematuration culture with 1 micromol/L, 5 micromol/L, and 10 micromol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls.


CONCLUSIONS
Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.
Jee
Byung Chul
BC
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, South Korea.
Chen
Hai-Ying
HY
Chian
Ri-Cheng
RC
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology; D006064:Gonadotropins, Equine / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D011247:Pregnancy; D015363:Quinolones / Q000494:pharmacology*
D006064:Gonadotropins, Equine; D010726:Phosphodiesterase Inhibitors; D015363:Quinolones; C021294:cilostamide