Production of bacterial cellulose by Gluconacetobacter sp. RKY5 isolated from persimmon vinegar.
The optimum fermentation medium for the production of bacterial cellulose (BC) by a newly isolated Gluconacetobacter sp. RKY5 was investigated. The optimized medium composition for cellulose production was determined to be 15 g/L glycerol, 8 g/L yeast extract, 3 g/L K2HPO4, and 3 g/L acetic acid. Under these optimized culture medium, Gluconacetobacter sp. RKY5 produced 5.63 g/L of BC after 144 h of shaken culture, although 4.59 g/L of BC was produced after 144 h of static culture. The amount of BC produced by Gluconacetobacter sp. RKY5 was more than 2 times in the optimized medium found in this study than in a standard Hestrin and Shramm medium, which was generally used for the cultivation of BC-producing organisms.
Department of Material Chemical and Biochemical Engineering, Chonnam National University, 500-757 Gwangju, Korea.
Soo-Yeon
SY
Kim
Jin-Nam
JN
Kim
Young-Jung
YJ
Wee
Don-Hee
DH
Park
Hwa-Won
HW
Ryu
D019342:Acetic Acid / Q000378:metabolism*; D019149:Bioreactors / Q000382:microbiology*; D018929:Cell Culture Techniques / Q000379:methods*; D002482:Cellulose / Q000378:metabolism*; D029788:Diospyros / Q000382:microbiology*; D041821:Gluconacetobacter / Q000145:classification* / Q000378:metabolism*; D013045:Species Specificity
D002482:Cellulose; D019342:Acetic Acid