Submerged culture screening of two strains of Streptomyces sp. with high keratinolytic activity.
Keratinases can be used for the production of potentially important hydrolyzed proteins and chemicals. This study investigated the keratinolytic activity of Streptomyces sp on keratinaceous materials like wool. High levels of proteolytic and keratinolytic activity were obtained after 96 h of culture when two Streptomyces sp strains were grown on basal medium containing mineral salts and 3% (w/v) of defatted wool as a source of energy, carbon, and nitrogen. The cell-free culture filtrates exhibited rapid proteolytic digestion of keratin powder. Currently, the authors are testing whether the enzymatic activity obtained is in fact keratinolytic, and not only an alkaline protease activity.
Departamento de Bioprocesos, Unidad Profesional Interdisciplinaria de Biotechnologís (UPIBI/IPN) del Institute Politécnico Nacional, Av. Acueducto s/n Barrio La Laguna, Ticomán, Zacatenco, 07340-México, D.F.
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Garcia-Kirchner
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Bautista-Ramirez
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Segura-Granados