Isoliquiritigenin inhibits cell proliferation by a heme oxygenase-dependent pathway in rat hepatic stellate cells.
We evaluated whether the antiproliferative effects of isoliquiritigenin (ISL) on rat hepatic stellate cells (HSCs) are related to the induction of heme oxygenase 1 (HO-1) expression. ISL significantly inhibited serum- or growth factor-induced HSC proliferation. The inhibition of platelet-derived growth factor (PDGF)-induced proliferation by ISL was associated with the mitogen-activated protein kinase and phosphatidylinositol 3-kinase-Akt-p70 (S6K) pathways. ISL induced the expression of HO-1 in HSCs. Using the chemical inhibitor tin protoporphyrin, we also found that the inhibitory action of ISL on PDGF-induced proliferation is mediated by HO-1. These data suggest that HO-1 expression is responsible for the antiproliferative effect of ISL on HSCs.
National Institute of Toxicological Research, Korea Food and Drug Administration, Seoul, South Korea.
Sun Wook
SW
Woo
Sung Hee
SH
Lee
Geonil
G
Ko
Youn-Chul
YC
Kim
Dong Hwan
DH
Sohn
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D047188:Chalcones / Q000302:isolation & purification / Q000494:pharmacology*; D035782:Dalbergia / Q000737:chemistry*; D051547:Heme Oxygenase-1 / Q000378:metabolism*; D008099:Liver / Q000166:cytology; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D051381:Rats
D047188:Chalcones; D010982:Platelet-Derived Growth Factor; C040920:isoliquiritigenin; D051547:Heme Oxygenase-1