Circadian and ultradian rhythms in the crayfish caudal photoreceptor.
The study of circadian clocks in crustaceans has led to the hypothesis of a distributed circadian system of pacemakers. In this review, we investigate the role of the crayfish caudal photoreceptor (CPR) as a candidate to form part of this pacemaking circadian system. Two circadian rhythms are documented for CPR electrical activity. These rhythms correspond to the spontaneous and light-induced discharge of action potentials. The intrinsic characterization of the rhythms is made through the analysis of the firing rate of the corresponding action potentials. The discharges were extracellularly recorded in the isolated 6th abdominal ganglion (AG) in an organ culture kept at constant temperature for up to 5 days. For preparations kept in the dark, spontaneous activity varies in a circadian manner, with a period of 24.7 h and the acrophase at subjective nighttime (2140). For light-induced activity, pulses of constant intensity applied regularly throughout the 24-h cycle show that the firing rate at peak and latency vary rhythmically. The period for this rhythm is 24.24 h and the acrophase is at subjective dawn (0326). Additionally, an ultradian rhythm of a approximately 12-h period was observed for both rhythms. When tested with light pulses of different intensities, the CPR responsiveness at night is almost one log unit greater than in daytime. The effect of temperature on both activities is also described. The phase-shift caused by temperature for these circadian rhythms depends on the application time. These results show that the 6th AG is capable of generating a circadian rhythm of electrical activity in the CPR, which in turn is likely to be part of the crayfish circadian system. A possible interaction of different pacemakers forming the distributed circadian system is also discussed. The role of serotonin as a possible modulator of the CPR electrical activity is documented. In addition, the level of the 5-HT(1A) receptors displays a diurnal rhythm in the 6th AG, with the acrophase at twilight (1849). We suggest that the 5-HT(1A) receptor does participate in this modulation. Finally, the hypothesis of the expression of two circadian oscillators in a single identified neuron is presented.
Rodríguez-Sosa
Leonardo
L
División de Estudios de Posgrado, Facultad de Medicina, Universidad Nacional Autónoma de México, México, D. F., México. lrsosa@servidor.unam.mx
Calderón-Rosete
Gabina
G
Flores
Gonzalo
G
D017371:8-Hydroxy-2-(di-n-propylamino)tetralin / Q000031:analogs & derivatives / Q000378:metabolism / Q000494:pharmacology; D000204:Activity Cycles / Q000502:physiology*; D000818:Animals; D003400:Astacoidea / Q000502:physiology*; D001683:Biological Clocks / Q000502:physiology; D002940:Circadian Rhythm / Q000502:physiology*; D017952:Ganglia, Invertebrate / Q000166:cytology / Q000502:physiology; D008029:Lighting; D009474:Neurons / Q000502:physiology; D017956:Photoreceptor Cells, Invertebrate / Q000502:physiology*; D044282:Receptor, Serotonin, 5-HT1A / Q000502:physiology; D012701:Serotonin / Q000502:physiology; D013696:Temperature; D014316:Tritium
D014316:Tritium; D044282:Receptor, Serotonin, 5-HT1A; D012701:Serotonin; C011799:5-hydroxy-2-N,N-dipropylaminotetralin; D017371:8-Hydroxy-2-(di-n-propylamino)tetralin