Effects of N-application on utilization of 15N and 13C and quality in two wheat cultivars.
We studied N uptake and distribution in wheat, and the incorporation of nitrogen and carbon into gluten and non-gluten proteins using a double-labelling approach with 15N and 13C. Doses of N-fertilizer were split and applied at emergence, onset of stem elongation, and heading at rates of 280/140/140 mg N pot(-1), respectively simulating 90/45/45 kg N ha(-1). Five different combinations of N-fertilizations containing no or 10 % 15N were performed. The recovery of 15N added at the stages emergence, stem elongation or heading were 42, 60, and 64 %. Application of 15N at all three stages yielded in 51 % recovery. Remobilisation of straw N was greater for Golia. The 15N concentration in gluten proteins of Golia show higher values than Gonen. The ratio of 15N gluten/15N non-gluten proteins of Golia were higher, which implies a lower non-gluten protein activity during grain filling. The 13C concentration in gluten and non-gluten proteins did not differ between both cultivars.
Department of Crop Science in the Tropics and Subtropics, Humboldt Universitat zu Berlin, Berlin, Germany. klaus-peter.goetz@agrar.hu-berlin.de
Klaus-Peter
KP
Gotz
Helmut
H
Herzog
Osman
O
Erekul
D000383:Agriculture / Q000379:methods*; D002247:Carbon Isotopes / Q000032:analysis; D018556:Crops, Agricultural / Q000378:metabolism; D005983:Glutens / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D009584:Nitrogen / Q000378:metabolism*; D009587:Nitrogen Isotopes / Q000032:analysis; D014674:Plant Proteins, Dietary / Q000737:chemistry / Q000378:metabolism*; D014908:Triticum / Q000378:metabolism*
D002247:Carbon Isotopes; D009587:Nitrogen Isotopes; D014674:Plant Proteins, Dietary; D005983:Glutens; D009584:Nitrogen