Analysis of collagen distribution in human crown dentin by confocal laser scanning microscopy.
The authors used confocal laser scanning microscope to analyze human crown dentin. Specimens from 10 teeth were divided in two groups, one of which was decalcified and stained with hematoxylin and eosin. In the second group an undecalcified section was analyzed. Both groups were scanned by confocal microscope to generate optically sectioned images. All of the analyzed samples presented an intense autofluorescent that was ascribed to collagens. The degree of autofluorescence intensity was variable and might be due to collagen expression. The results indicate that a confocal microscope may be of help in analyzing and defining the nature and extent of collagen fibrils in human dentin.
Department of Odontostomatology and Surgery, Faculty of Medicine, University of Bari, Bari, Italy. alucchese@hotmail.com
Alberta
A
Lucchese
Giovanni Pietro
GP
Pilolli
Massimo
M
Petruzzi
Vito
V
Crincoli
Michele
M
Scivetti
Gianfranco
G
Favia
D003094:Collagen / Q000032:analysis*; D003804:Dentin / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D019228:Tooth Crown / Q000737:chemistry*
D003094:Collagen