Plasmodium in the postgenomic era: new insights into the molecular cell biology of malaria parasites.
In this review, we bring together some of the approaches toward understanding the cellular and molecular biology of Plasmodium species and their interaction with their host red blood cells. Considerable impetus has come from the development of new methods of molecular genetics and bioinformatics, and it is important to evaluate the wealth of these novel data in the context of basic cell biology. We describe how these approaches are gaining valuable insights into the parasite-host cell interaction, including (1) the multistep process of red blood cell invasion by the merozoite; (2) the mechanisms by which the intracellular parasite feeds on the red blood cell and exports parasite proteins to modify its cytoadherent properties; (3) the modulation of the cell cycle by sensing the environmental tryptophan-related molecules; (4) the mechanism used to survive in a low Ca(2+) concentration inside red blood cells; (5) the activation of signal transduction machinery and the regulation of intracellular calcium; (6) transfection technology; and (7) transcriptional regulation and genome-wide mRNA studies in Plasmodium falciparum.
Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, CEP 05508-900, São Paulo, SP, Brazil.
Celia R S
CR
Garcia
Mauro F
MF
de Azevedo
Gerhard
G
Wunderlich
Alexandre
A
Budu
Jason A
JA
Young
Lawrence
L
Bannister
D000818:Animals; D002118:Calcium / Q000378:metabolism; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002470:Cell Survival / Q000235:genetics*; D004912:Erythrocytes / Q000469:parasitology*; D005786:Gene Expression Regulation / Q000235:genetics; D018503:Genome, Protozoan / Q000235:genetics; D006790:Host-Parasite Interactions / Q000235:genetics*; D008967:Molecular Biology / Q000639:trends; D010961:Plasmodium / Q000235:genetics* / Q000502:physiology* / Q000648:ultrastructure; D015398:Signal Transduction / Q000235:genetics
D018797:Cell Cycle Proteins; D002118:Calcium