Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme.
Aside from its enzymatic function in the glycolytic pathway, alpha-enolase (ENO1) has been implicated in numerous diseases, including metastatic cancer, autoimmune disorders, ischaemia and bacterial infection. The disease-related roles of ENO1 are mostly attributed to its immunogenic capacity, DNA-binding ability and plasmin(ogen) receptor function, which are significantly affected by its three-dimensional structure and surface properties, rather than its enzymatic activity. Here, the crystal structure of human ENO1 (hENO1) is presented at 2.2 A resolution. Despite its high sequence similarity to other enolases, the hENO1 structure exhibits distinct surface properties, explaining its various activities, including plasmin(ogen) and DNA binding.
BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology and Division of Nanobiotechnology, Korea University of Science and Technology (UST), Yuseong, Daejeon 305-333, Republic of Korea.
Hyo Jin
HJ
Kang
Suk-Kyeong
SK
Jung
Seung Jun
SJ
Kim
Sang J
SJ
Chung
D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020134:Catalytic Domain; D018360:Crystallography, X-Ray; D017931:DNA Primers / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D005341:Fibrinolysin / Q000378:metabolism; D006801:Humans; D008958:Models, Molecular; D010751:Phosphopyruvate Hydratase / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D010958:Plasminogen / Q000378:metabolism; D017433:Protein Structure, Secondary; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D055672:Static Electricity; D025521:Tumor Suppressor Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
D014408:Biomarkers, Tumor; D017931:DNA Primers; D018076:DNA, Complementary; D004268:DNA-Binding Proteins; D011994:Recombinant Proteins; D025521:Tumor Suppressor Proteins; D010958:Plasminogen; D005341:Fibrinolysin; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase