Investigations of oxygen transfer into Penicillium chrysogenum pellets by microprobe measurements.
A microcoaxial needle sensor with a tip diameter of ca. 0.7 microm was used as a microprobe to measure profiles of dissolved oxygen tension (DOT) within fixed pellets of Penicillium chrysogenum as a function of the DOT level around the pellet, in the presence and absence of bulk convective flow and turbulence. The investigations indicate that the oxygen transfer mechanism is complex. The results were interpreted by assuming the penetration convective flow into the entire pellet and penetration of turbulence into the outer range. A model was developed which was able to describe the measured DOT profiles very well. The model takes into account molecular and turbulent diffusion as well as convective flow as transfer mechanisms inside of the pellet. Structures of pellets used for microprobe measurements were evaluated by histological investigations. Considerable variations of mycelial density with radius within the pellets were found.
Heinrich Frings GmbH and CoKG Jonas Cahn Str g, D-5300 Bonn 1, Federal Republic of Germany.
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Wittier
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Baumgartl
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Lübbers
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Schügerl