Highly efficient and low-cost method to isolate human blood monocytes with high purity.
Several techniques are available to purify circulating blood monocytes for research. CD14-containing MicroBeads are suitable and reliable tools to reproducibly isolate human monocytes with a high purity but are quite expensive. This report describes that a comparable number of highly pure monocytes can be isolated from samples using up to tenfold lower amounts of CD14-MicroBeads. MicroBeads are widely used to isolate different cell populations and with this report more researchers may be encouraged to use this highly efficient, low-cost and thus affordable method to pursue their scientific goals.
Department of Internal Medicine I, University of Regensburg, Regensburg, Germany.
Fabian
F
Stögbauer
Markus
M
Neumeier
Johanna
J
Weigert
Josef
J
Wanninger
Margot
M
Grandl
Karla
K
Lehle
Gerd
G
Schmitz
Charalampos
C
Aslanidis
Andreas
A
Schäffler
Jürgen
J
Schölmerich
Christa
C
Buechler
D000328:Adult; D005260:Female; D005434:Flow Cytometry / Q000191:economics; D006801:Humans; D018189:Immunomagnetic Separation / Q000191:economics / Q000379:methods*; D018950:Lipopolysaccharide Receptors / Q000032:analysis*; D008297:Male; D008863:Microspheres; D009000:Monocytes / Q000276:immunology*
D018950:Lipopolysaccharide Receptors