Severe Vitamin E deficiency exacerbates acute hyperoxic lung injury associated with increased oxidative stress and inflammation.
Hyperoxia causes acute lung injury along with an increase of oxidative stress and inflammation. It was hypothesized that vitamin E deficiency might exacerbate acute hyperoxic lung injury. This study used alpha-tocopherol transfer protein knockout (alpha-TTP KO) mice fed a vitamin E-deficient diet (KO E(-) mice) as a model of severe vitamin E deficiency. Compared with wild-type (WT) mice, KO E(-) mice showed a significantly lower survival rate during hyperoxia. After 72 h of hyperoxia, KO E(-) mice had more severe histologic lung damage and higher values of the total cell count and the protein content of bronchoalveolar lavage fluid (BALF) than WT mice. IL-6 mRNA expression in lung tissue and the levels of 8-iso-prostaglandin F(2alpha) (8-iso-PGF(2alpha)) in both lungs and BALF were higher in KO E(-) mice than in WT mice. It was concluded that severe vitamin E deficiency exacerbates acute hyperoxic lung injury associated with increased oxidative stress or inflammation.
Department of Pediatrics, Perinatal Center, Osaka Medical College, Takatsuki, Osaka, Japan. ped081@poh.osaka-med.ac.jp
Shigeo
S
Yamaoka
Han-Suk
HS
Kim
Tohru
T
Ogihara
Shinya
S
Oue
Kimitaka
K
Takitani
Yasukazu
Y
Yoshida
Hiroshi
H
Tamai
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D002352:Carrier Proteins / Q000378:metabolism; D005786:Gene Expression Regulation; D018496:Hyperoxia; D007249:Inflammation; D015850:Interleukin-6 / Q000378:metabolism; D008168:Lung / Q000473:pathology; D055370:Lung Injury; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008954:Models, Biological; D018384:Oxidative Stress; D013997:Time Factors; D014811:Vitamin E Deficiency / Q000235:genetics*
D002352:Carrier Proteins; D015850:Interleukin-6; C017763:alpha-tocopherol transfer protein