Immunohistochemical evaluation of 95 bone marrow reactive plasmacytoses.
We histologically and immunohistochemically studied 95 bone marrow (BM) reactive plasmacytoses. Ten biopsies from plasma cell myeloma (PCM) patients served as a control group. In addition, we studied 10 monoclonal gammopathy of undetermined significance (MGUS) cases. Histologically, plasmacytosis varied between 5% and 25% with an interstitial pattern of plasma cell (PC) distribution being characteristically displayed. Immunohistochemically, we did not find any CD56/NCAM nor cyclin D1 expression in all biopsies (95 of 95, 100%), not even a weak, doubtful one; PCs were all polyclonal and CD138 positive. On the contrary, myeloma-associated PCs showed monoclonality for kappa- or lambda- light chain and strong CD56/NCAM immunoreactivity (8 of 10, 80%); four of them were cyclin D1 positive. Osteoblasts exhibited similar CD56/NCAM expression in both groups. Our data confirm the diagnostic utility of CD56/NCAM in the phenotypic characterization of polyclonal plasma cells, suggesting an important role of this particular immunomarker in the BM trephine study of polyclonal versus neoplastic plasmacytic infiltrations.
Ioannou
Maria G
MG
Department of Pathology, Medical School, University of Thessaly, Larisa, Greece.
Stathakis
Efstathios
E
Lazaris
Andreas C
AC
Papathomas
Thomas
T
Tsiambas
Evangelos
E
Koukoulis
George K
GK
D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D019002:CD56 Antigen / Q000378:metabolism*; D019938:Cyclin D1 / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007146:Immunoglobulin lambda-Chains / Q000378:metabolism*; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D010950:Plasma Cells / Q000378:metabolism* / Q000473:pathology; D012189:Retrospective Studies
C530104:CCND1 protein, human; D019002:CD56 Antigen; D007146:Immunoglobulin lambda-Chains; D019006:Neural Cell Adhesion Molecules; D019938:Cyclin D1