Biochemical individuality reflected in chromatographic, electrophoretic and mass-spectrometric profiles.
This review discusses the current trends in molecular profiling for the emerging systems biology applications. Historically, the methodological developments in separation science were coincident with the availability of new ionization techniques in mass spectrometry. Coupling miniaturized separation techniques with technologically-advanced MS instrumentation and the modern data processing capabilities are at the heart of current platforms for proteomics, glycomics and metabolomics. These are being featured here by the examples from quantitative proteomics, glycan mapping and metabolomic profiling of physiological fluids.
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA. novotny@indiana.edu
Milos V
MV
Novotny
Helena A
HA
Soini
Yehia
Y
Mechref
D000818:Animals; D002849:Chromatography, Gas / Q000379:methods*; D002853:Chromatography, Liquid / Q000379:methods*; D004586:Electrophoresis / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D023421:Models, Animal