Aptamer-facilitated biomarker discovery (AptaBiD).
Here we introduce a technology for biomarker discovery in which (i) DNA aptamers to biomarkers differentially expressed on the surfaces of cells being in different states are selected; (ii) aptamers are used to isolate biomarkers from the cells; and (iii) the isolated biomarkers are identified by means of mass spectrometry. The technology is termed aptamer-facilitated biomarker discovery (AptaBiD). AptaBiD was used to discover surface biomarkers that distinguish live mature and immature dendritic cells. We selected in vitro two DNA aptamer pools that specifically bind to mature and immature dendritic cells with a difference in strength of approximately 100 times. The aptamer pools were proven to be highly efficient in flow- and magnetic-bead-assisted separation of mature cells from immature cells. The two aptamer pools were then used to isolate biomarkers from the cells. The subsequent mass spectrometry analysis of the isolated proteins revealed unknown biomarkers of immature and mature dendritic cells.
Department of Chemistry, York University, Toronto, Ontario M3J 1P3, Canada.
Maxim V
MV
Berezovski
Matthias
M
Lechmann
Michael U
MU
Musheev
Tak W
TW
Mak
Sergey N
SN
Krylov
D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry* / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis* / Q000378:metabolism; D002469:Cell Separation / Q000379:methods; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D003713:Dendritic Cells / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D015723:Gene Library; D008280:Magnetics; D051379:Mice; D008822:Mice, Transgenic
D052157:Aptamers, Nucleotide; D015415:Biomarkers; D004277:DNA, Single-Stranded