Muscle fiber size and function in elderly humans: a longitudinal study.
Cross-sectional studies are likely to underestimate age-related changes in skeletal muscle strength and mass. The purpose of this longitudinal study was to assess whole muscle and single muscle fiber alterations in the same cohort of 12 older (mean age: start of study 71.1+/-5.4 yr and end of study 80+/-5.3 yr) volunteers (5 men) evaluated 8.9 yr apart. No significant changes were noted at follow-up in body weight, body mass index, and physical activity. Muscle strength, evaluated using isokinetic dynamometry, and whole muscle specific force of the knee extensors were significantly lower at follow-up. This was accompanied by a significant reduction (5.7%) in cross-sectional area of the total anterior muscle compartment of the thigh as evaluated by computed tomography. Muscle histochemistry showed no significant changes in fiber type distribution or fiber area. Experiments with chemically skinned single muscle fibers (n=411) demonstrated no change in type I fiber size but an increase in IIA fiber diameter. A trend toward an increase in maximal force in both fiber types was observed. Maximum unloaded shortening velocity did not change. In conclusion, single muscle fiber contractile function may be preserved in older humans in the presence of significant alterations at the whole muscle level. This suggests that surviving fibers compensate to partially correct muscle size deficits in an attempt to maintain optimal force-generating capacity.
Frontera
Walter R
WR
Nutrition, Exercise Physiology, and Sarcopenia Laboratory, Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, 711 Washington St., Boston, MA 02111, USA.
Reid
Kieran F
KF
Phillips
Edward M
EM
Krivickas
Lisa S
LS
Hughes
Virginia A
VA
Roubenoff
Ronenn
R
Fielding
Roger A
RA
D000273:Adipose Tissue / Q000502:physiology; D000368:Aged; D000375:Aging / Q000502:physiology*; D048429:Cell Size; D005260:Female; D006651:Histocytochemistry; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D018485:Muscle Fibers, Skeletal / Q000502:physiology / Q000648:ultrastructure*; D009126:Muscle Relaxation / Q000502:physiology; D053580:Muscle Strength / Q000502:physiology; D018482:Muscle, Skeletal / Q000166:cytology / Q000254:growth & development; D018995:Myosin Heavy Chains / Q000378:metabolism; D013848:Thigh / Q000033:anatomy & histology; D014057:Tomography, X-Ray Computed
D018995:Myosin Heavy Chains