Electrochemical evaluation of lectin-sugar interaction on gold electrode modified with colloidal gold and polyvinyl butyral.
In this work, ConA and CramoLL lectins were immobilized on gold nanoparticles (AuNp) with polyvinyl butyral (PVB), and adsorbed on the surface of gold (Au) electrodes. Electrochemical impedance spectroscopy (EIS), in the frequency range from 100mHz to 100KHz, and cyclic voltammetry (CV), from -0.2 to 0.7V, were performed on these electrodes, in phosphate buffer (PBS) solution containing 10mM K(3)[Fe(CN)(6)]/K(4)[Fe(CN)(6)] (1:1) mixture as a redox probe. EIS and CV measurements showed that redox probe reactions on the modified Au electrodes were partially blocked due to the adsorption of AuNp-ConA-PVB and AuNp-CramoLL-PVB. SEM images showed the presence of aggregates of AuNp-ConA on PVB spherules in a tridimensional structure on the surface of the Au electrode. Bovine serum albumin (BSA) was adsorbed on the AuNp-Lectin-PVB modified electrode in order to block the remaining free gold sites. Both EIS and CV techniques yielded results that confirm positive responses of the lectins to ovalbumin agglutination. These results indicate an improvement of the sensitivity for detection of sugars that can be applicable to construction of a biosensor sensitive to glycoproteins in solution.
Departamento de Química Fundamental - Universidade Federal de Pernambuco, 50670-901 Recife, PE, Brazil.
Maria D L
MD
Oliveira
Maria T S
MT
Correia
Luana C B B
LC
Coelho
Flamarion B
FB
Diniz
D000818:Animals; D015374:Biosensing Techniques; D002417:Cattle; D003208:Concanavalin A / Q000737:chemistry / Q000378:metabolism*; D017097:Electric Impedance; D004563:Electrochemistry; D004566:Electrodes; D005947:Glucose / Q000737:chemistry / Q000378:metabolism*; D017979:Gold Colloid / Q000737:chemistry*; D053768:Metal Nanoparticles; D010047:Ovalbumin / Q000737:chemistry / Q000378:metabolism; D011145:Polyvinyls / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000378:metabolism
D017979:Gold Colloid; D011145:Polyvinyls; D003208:Concanavalin A; D012710:Serum Albumin, Bovine; C027464:polyvinylbutyral; D010047:Ovalbumin; D005947:Glucose