The role of adipose protein derived hydrogels in adipogenesis.
Biomaterials that induce adipogenesis may ultimately serve as alternatives to traditional tissue reconstruction and regeneration techniques. In addition, these materials can provide environments for studying factors that regulate adipogenesis. The present study investigates the potential of adipose-derived matrices to induce adipogenesis in vitro and in vivo. Solutions containing basement membrane proteins and growth factors were extracted from subcutaneous adipose tissue. These extracts could be induced to form gels by either incubating the solutions at 37 degrees C or adjusting the pH to 4.0. The adipose extracts promoted rapid preadipocyte aggregation and formation of lipid-loaded colonies in vitro. Differentiation on adipose-derived gels was greater than tissue culture dishes and the tumor-derived product Matrigel (p < 0.05). Significant adipose formation was observed when adipose-derived gels were implanted around a rat epigastric pedicle bundle. Adipose levels in these gels were significantly greater than Matrigel (p < 0.05). The duration of adipose formation depended on the mechanism for gelling the solutions, with acid gelled matrices having greater adipose levels at 6 weeks than temperature gelled matrices. These adipose-derived hydrogels promote rapid adipogenesis in vitro and in vivo. They may lead to new materials for adipose tissue engineering, and provide an environment for studying cell-matrix interactions in adipogenesis.
Uriel
Shiri
S
Pritzker Institute of Biomedical Science and Engineering, Department of Biomedical Engineering, Illinois Institute of Technology, 10 W. 32nd Street, Engineering 1 Room 116, Chicago, IL 60616-3793, USA.
Huang
Jung-Ju
JJ
Chung Gung Memorial Hospital, Chang Gung Medical College, Chang Gung University, Department of Plastic and Reconstructive Surgery, Taoyuan, 5, Fu-Hsing Street, Kweishan, Taiwan.
Moya
Monica L
ML
Pritzker Institute of Biomedical Science and Engineering, Department of Biomedical Engineering, Illinois Institute of Technology, 10 W. 32nd Street, Engineering 1 Room 116, Chicago, IL 60616-3793, USA.
Francis
Megan E
ME
Pritzker Institute of Biomedical Science and Engineering, Department of Biomedical Engineering, Illinois Institute of Technology, 10 W. 32nd Street, Engineering 1 Room 116, Chicago, IL 60616-3793, USA; Department of Research, Hines V.A. Hospital, Hines, IL, USA.
Wang
Rui
R
Pritzker Institute of Biomedical Science and Engineering, Department of Biomedical Engineering, Illinois Institute of Technology, 10 W. 32nd Street, Engineering 1 Room 116, Chicago, IL 60616-3793, USA.
Chang
Shu-Ying
SY
Chung Gung Memorial Hospital, Chang Gung Medical College, Chang Gung University, Department of Plastic and Reconstructive Surgery, Taoyuan, 5, Fu-Hsing Street, Kweishan, Taiwan.
Cheng
Ming-Huei
MH
Chung Gung Memorial Hospital, Chang Gung Medical College, Chang Gung University, Department of Plastic and Reconstructive Surgery, Taoyuan, 5, Fu-Hsing Street, Kweishan, Taiwan.
Brey
Eric M
EM
Pritzker Institute of Biomedical Science and Engineering, Department of Biomedical Engineering, Illinois Institute of Technology, 10 W. 32nd Street, Engineering 1 Room 116, Chicago, IL 60616-3793, USA; Department of Research, Hines V.A. Hospital, Hines, IL, USA.
D000273:Adipose Tissue / Q000737:chemistry* / Q000166:cytology*; D000818:Animals; D002454:Cell Differentiation; D016220:Fibroblast Growth Factor 1 / Q000032:analysis; D016222:Fibroblast Growth Factor 2 / Q000032:analysis; D020100:Hydrogels; D011506:Proteins / Q000737:chemistry*; D051381:Rats
D020100:Hydrogels; D011506:Proteins; D016222:Fibroblast Growth Factor 2; D016220:Fibroblast Growth Factor 1