High-affinity IgE receptor-beta chain expression in human mast cells.
The high-affinity IgE receptor (FcepsilonRI)-beta gene is one of the atopy-associated genes, but its biological significance is largely unknown. In this study, we generated the anti-FcepsilonRI-beta chain antibody to clarify beta-chain protein expression in human mast cells. The FcepsilonRI-beta antibody showed specific binding to a 27 kDa protein with Western blotting and membrane bound immunostaining using cultured mast cells. Monomeric IgE sensitization increased beta-chain expression as well as mature alpha-chain expression in mast cells. Upregulation of beta-chain expression with monomeric IgE treatment suggests possible roles of FcepsilonRI-beta protein as an atopy-related molecule.
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan. akimatsu@ophth.kpu-m.ac.jp
Akira
A
Matsuda
Yoshimichi
Y
Okayama
Nobuyuki
N
Ebihara
Norihiko
N
Yokoi
Peisong
P
Gao
Junji
J
Hamuro
Julian M
JM
Hopkin
Shigeru
S
Kinoshita
D000906:Antibodies / Q000276:immunology*; D015153:Blotting, Western; D002460:Cell Line; D002478:Cells, Cultured; D005434:Flow Cytometry; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008407:Mast Cells / Q000276:immunology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017455:Receptors, IgE / Q000235:genetics / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation
D000906:Antibodies; C502447:MS4A2 protein, human; D012333:RNA, Messenger; D017455:Receptors, IgE; D007073:Immunoglobulin E