Haemophilus paragallinarum haemagglutinin: role in adhesion, serotyping and pathogenicity.
It is suggested that Haemophilus paragallinarum requires at least three haemagglutinins for adhesion during infection. This paper reports the partial purification and characterization of the HA-L haemagglutinin from H. paragallinarum strain 46-C3, a heat sensitive, trypsin sensitive haemagglutinin that has been shown to be the serovar specific haemagglutinin in this organism. Using the pl and molecular mass obtained, it was shown that this protein shares similarities with other types of adhesins found in Gram-negative bacteria. The haemagglutination assay conditions were optimized at pH 7.5 at 37 degrees C. It was also shown that activity is enhanced by the addition of Ca2+ and Mn2+ ions.
Barnard
T G
TG
Department of Microbial, Biochemical and Food Biotechnology, Faculty of Natural and Agricultural Science, University of the Free State, P.O. Box 339, Bloemfontein 9301, South Africa.
Van Heerden
E
E
Bragg
R R
RR
Albertyn
J
J
D000818:Animals; D001422:Bacterial Adhesion / Q000502:physiology*; D001426:Bacterial Proteins / Q000302:isolation & purification / Q000502:physiology; D004591:Electrophoresis, Polyacrylamide Gel / Q000662:veterinary; D006192:Haemophilus Infections / Q000382:microbiology / Q000662:veterinary*; D044134:Haemophilus paragallinarum / Q000254:growth & development / Q000472:pathogenicity / Q000502:physiology*; D006386:Hemagglutination Tests / Q000662:veterinary; D006388:Hemagglutinins / Q000302:isolation & purification* / Q000502:physiology; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D012703:Serotyping / Q000662:veterinary; D013696:Temperature
D001426:Bacterial Proteins; D006388:Hemagglutinins