Development of a culture medium for large-scale production of cellulolytic enzymes by Trichoderma reesei.
Culture filtrates of CL-847 strain of Trichoderma reesei grown on different carbon sources have been compared. The highest enzyme production is obtained with Whatman C 41 cellulose: 17.9 mg/mL of soluble proteins and 13.7 units of filter paper (FP) activity. Wood pulps gave lower production values and more viscous culture media. About one-third of maximal enzyme production is obtained on lactose as the sole carbon source. Addition of 0.5% cellulosic inducer to 6% lactose media enhances enzyme production up to the following levels: 14.1 mg/mL of soluble proteins and 8.4 units of FP activity.
Institut Fraņais du Pétrole, 1 et 4 avenue de Bois-Préau, 92506-Rueil-Malmaison, France.
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Warzywoda
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Ferre
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Pourquie