Determination of d-limonene in adipose tissue by gas chromatography-mass spectrometry.
We developed a novel method for analyzing d-limonene levels in adipose tissue. Fat samples were subjected to saponification followed by solvent extraction. d-Limonene in the sample extract was analyzed using gas chromatography-mass spectrometry (GC-MS) with selected ion monitoring. Linear calibration curves were established over the mass range of 79.0-2529 ng d-limonene per 0.1g of adipose tissue. Satisfactory within-day precision (R.S.D. 6.7-9.6%) and accuracy (%difference of -2.7 to 3.8%) and between-day precision (R.S.D. 6.0-10.7%) and accuracy (%difference of 1.8-2.6%) were achieved. The assay was successfully applied to human fat biopsy samples from a d-limonene feeding trial.
Miller
Jessica A
JA
Department of Nutritional Sciences, The University of Arizona, Tucson, AZ 85721, USA.
Hakim
Iman A
IA
Thomson
Cynthia
C
Thompson
Patricia
P
Chow
H-H Sherry
HH
D000273:Adipose Tissue / Q000737:chemistry*; D002138:Calibration; D053138:Cyclohexenes / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D000077222:Limonene; D013729:Terpenes / Q000737:chemistry*
D053138:Cyclohexenes; D013729:Terpenes; D000077222:Limonene