In vivo evaluation of bonding ability and biocompatibility of a novel biodegradable glue consisting of tartaric acid derivative and human serum albumin.
We recently developed a novel biological glue from tartaric acid derivative (TAD) with two active ester groups and human serum albumin (HSA), named TAD-A. In this study, in vivo experiments were performed to investigate clinical applicability of TAD-A. TAD was prepared by reacting carboxyl groups of tartaric acid with N-hydroxysuccinimide in the presence of carbodiimide. Bonding strength was evaluated by using mouse skin closed with TAD-A of different TAD concentrations from 0.1 to 0.5 mmol in 0.8 mg of 44 w/w % HSA solution. Commercially available glues such as fibrin and aldehyde-based glue were used for comparison. We found that TAD-A's bonding strength increased significantly with TAD-A concentration. The bonding strength of 0.5 mmol of TAD-A in 0.8 mg of 44 w/w % HSA solution was significantly higher than that of fibrin or aldehyde-based glue (p < 0.01), and that of 0.3 mmol of TAD-A was significantly higher than of fibrin glue (p < 0.05). To determine toxicity, we implanted disks made from TAD-A of different TAD concentrations from 0.1 to 0.5 mmol in 0.8 mg of 44 w/w % HSA solution subcutaneously in mice. The inflammatory reaction in surrounding tissue increased with increasing TAD concentration, and then the disks were absorbed. In conclusion, TAD-A has sufficient bonding strength and comparatively low toxicity in clinical use of 0.3 mmol or less of TAD and 0.8 mL of 44 w/w % HSA solution.
Department of Orthopedic Surgery, Graduate School of Comprehensive Human Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba 305-8575, Japan.
Masashi
M
Iwasashi
Masataka
M
Sakane
Hirofumi
H
Saito
Tetsushi
T
Taguchi
Tetsuya
T
Tateishi
Naoyuki
N
Ochiai
D000447:Aldehydes / Q000737:chemistry; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D015718:Fibrin Tissue Adhesive / Q000737:chemistry; D006801:Humans; D007249:Inflammation; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D012709:Serum Albumin / Q000737:chemistry*; D013644:Tartrates / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D014014:Tissue Adhesives / Q000737:chemistry; D014945:Wound Healing
D000447:Aldehydes; D001672:Biocompatible Materials; D015718:Fibrin Tissue Adhesive; D012709:Serum Albumin; D013644:Tartrates; D014014:Tissue Adhesives; C029768:tartaric acid