Influence of local tetracycline on the microbiota of alveolar osteitis in rats.
The aim of the present study was to evaluate the effects of local tetracycline on the occurrence of alveolar osteitis in rats, and on the microbiota associated to this infection. Forty Wistar rats were randomly assigned to 4 groups (n=10): I - the rats had the maxillary right incisor extracted and the alveolar wound did not receive any treatment; II - adrenaline and Ringer-PRAS were introduced into the alveolar wound; III - the alveolar wound was irrigated with sterile saline; and IV - the alveolar wound was irrigated with an aqueous solution of tetracycline. Microbial samples from the alveolar wounds were collected 2 days after surgery and inoculated on blood agar (with and without 8 microg/mL of tetracycline) and other selective media, and were incubated in either aerobiosis or anaerobiosis at 37 degrees C, for 2 to 14 days. It was verified that tetracycline reduced the occurrence of alveolar osteitis in the rats and caused significant changes in the microbiota of the surgical sites, decreasing the number of anaerobes and increasing the participation of tetracycline-resistant and multi-resistant microorganisms.
Department of Surgery and Integrated Clinic, Dental School of Araçatuba, São Paulo State University, Araçatuba, SP, Brazil.
Joseane Maria Dias
JM
Bosco
Sérgio Ricardo
SR
de Oliveira
Alvaro Francisco
AF
Bosco
Christiane Marie
CM
Schweitzer
Elerson Gaetti
EG
Jardim Júnior
D000190:Actinomyces / Q000187:drug effects; D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D001439:Bacteroides / Q000187:drug effects; D015169:Colony Count, Microbial; D024901:Drug Resistance, Multiple, Bacterial; D004368:Dry Socket / Q000382:microbiology* / Q000517:prevention & control; D004755:Enterobacteriaceae / Q000187:drug effects; D016983:Enterococcus / Q000187:drug effects; D004837:Epinephrine / Q000627:therapeutic use; D005051:Eubacterium / Q000187:drug effects; D005673:Fusobacterium / Q000187:drug effects; D007180:Incisor / Q000601:surgery; D007552:Isotonic Solutions; D008297:Male; D010462:Peptostreptococcus / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D000077331:Ringer's Solution; D013492:Suppuration; D013752:Tetracycline / Q000627:therapeutic use*; D013753:Tetracycline Resistance; D007507:Therapeutic Irrigation; D014081:Tooth Extraction; D020390:Tooth Socket / Q000187:drug effects / Q000382:microbiology; D014662:Vasoconstrictor Agents / Q000627:therapeutic use; D014678:Veillonella / Q000187:drug effects
D000900:Anti-Bacterial Agents; D007552:Isotonic Solutions; D014662:Vasoconstrictor Agents; D000077331:Ringer's Solution; D013752:Tetracycline; D004837:Epinephrine