Overexpression of immediate early gene X-1 (IEX-1) enhances gamma-radiation-induced apoptosis of human glioma cell line, U87-MG.
Malignant gliomas are usually incurable even if adjuvant therapy is delivered after neurosurgical treatment. Therefore, to enhance their radiation-induced apoptosis, it is important to detect the mechanism(s) leading to the death of malignant glioma cells. We report that apoptosis was induced in a time-dependent manner after gamma-radiation and that irradiated U87-MG cells (human glioblastoma cell line) expressed immediate early gene X-1 (IEX-1) with p53. We also document that their apoptotic sensitivity to gamma-radiation was enhanced by the overexpression of IEX-1. Our findings suggest that IEX-1 may represent a new factor for the enhancement of radiation-induced apoptosis of human glioma cells.
Yamashita
Kentaro
K
Departments of Neurosurgery, Gifu University Graduate School of Medicine, Yanagido, Japan. kenta_mail_1127@yahoo.co.jp
Nakashima
Shigeru
S
You
Fukka
F
Hayashi
Shin-Ichiro
S
Iwama
Toru
T
D000199:Actins / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics / Q000528:radiation effects*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D017781:Genes, Immediate-Early; D016158:Genes, p53; D005910:Glioma / Q000235:genetics / Q000473:pathology* / Q000532:radiotherapy; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D008856:Microscopy, Fluorescence; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism; D014162:Transfection
D000199:Actins; D051017:Apoptosis Regulatory Proteins; C099815:IER3 protein, human; D008565:Membrane Proteins; D011065:Poly(ADP-ribose) Polymerases