Analysis of 13C labeling enrichment in microbial culture applying metabolic tracer experiments using gas chromatography-combustion-isotope ratio mass spectrometry.
The applicability of gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) for the quantification of 13C enrichment of proteinogenic amino acids in metabolic tracer experiments was evaluated. Measurement of the 13C enrichment of proteinogenic amino acids from cell hydrolyzates of Corynebacterium glutamicum growing on different mixtures containing between 0.5 and 10% [1-13C]glucose shows the significance of kinetic isotope effects in metabolic flux studies at low degree of labeling. We developed a method to calculate the 13C enrichment. The approach to correct for these effects in metabolic flux studies using delta13C measurement by GC-C-IRMS uses two parallel experiments applying substrate with natural abundance and 13C-enriched tracer substrate, respectively. The fractional enrichment obtained in natural substrate is subtracted from that of the enriched one. Tracer studies with C. glutamicum resulted in a statistically identical relative fractional enrichment of 13C in proteinogenic amino acids over the whole range of applied concentrations of [1-13C]glucose. The current findings indicate a great potential of GC-C-IRMS for labeling quantification in 13C metabolic flux analysis with low labeling degree of tracer substrate directly in larger scale bioreactors.
Biochemical Engineering Institute, Saarland University, 66123 Saarbruecken, Germany. e.heinzle@mx.uni-saarland.de
Elmar
E
Heinzle
Yongbo
Y
Yuan
Sathish
S
Kumar
Christoph
C
Wittmann
Matthias
M
Gehre
Hans-Herrmann
HH
Richnow
Patrick
P
Wehrung
Pierre
P
Adam
Pierre
P
Albrecht
D000596:Amino Acids / Q000032:analysis* / Q000378:metabolism; D002247:Carbon Isotopes / Q000032:analysis* / Q000378:metabolism; D048230:Corynebacterium glutamicum / Q000737:chemistry / Q000254:growth & development / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D005947:Glucose / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics; D007553:Isotope Labeling / Q000379:methods*; D007700:Kinetics
D000596:Amino Acids; D002247:Carbon Isotopes; D005947:Glucose