Sensitive and rapid method to determine trimetazidine in human plasma by liquid chromatography/tandem mass spectrometry.
A simple, sensitive, and rapid liquid chromatographic/tandem mass spectrometric (LC/MS/MS) method, using electrospray ionization, was developed and validated to quantify trimetazidine in human plasma using propranolol hydrochloride as an internal standard (IS). Samples were prepared by solid-phase extraction and analyzed without drying and reconstitution. The analyte and IS were chromatographed on a C18 reversed-phase column under isocratic conditions using 2 mM ammonium acetate (pH 3.5)-acetonitrile (40 + 60, v/v) as the mobile phase with a run time of 2.0 min. Quantitation was done on a triple-quadrupole mass analyzer API-3000, equipped with turbo ion spray interface and operating in multiple reaction monitoring mode to detect parent --> product ion (m/z 267.2 --> 181.4) transition. The method was validated for sensitivity, accuracy and precision, linearity, recovery, matrix effect, and stability. Linearity in plasma was observed over the concentration range of 1.5-300 ng/mL. Lower limit of quantification achieved was 1.5 ng/mL with precision < 10% using 10 microL injection volume. The mean relative recovery of analyte (97.36%) and IS (99.93%) was consistent and reproducible. Interbatch and intrabatch precision was < 8.0% and the accuracy determined was within +/- 8% in terms of relative error.
Gujarat University, Department of Chemistry, School of Sciences, Navrangpura, Ahmedabad 380 009, India.
Hiren N
HN
Mistri
Arvind G
AG
Jangid
Pranav
P
Shrivastav
D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D006801:Humans; D011786:Quality Control; D012015:Reference Standards; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods / Q000592:standards / Q000706:statistics & numerical data; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D014292:Trimetazidine / Q000097:blood* / Q000592:standards; D014665:Vasodilator Agents / Q000097:blood / Q000592:standards
D014665:Vasodilator Agents; D014292:Trimetazidine