Plantibody-mediated inhibition of the Potato leafroll virus P1 protein reduces virus accumulation.
The P1 protein of Potato leafroll virus (PLRV) is thought to play a major role in the replication cycle by promoting the maturation of the genome-linked virion protein VPg. To study the relevance of P1 and its autoproteolytic derivative P1-C25 in the viral life cycle, the V H and V L domains of monoclonal antibody mAbP1-1, raised against the C-terminus of P1, were used to develop a single chain variable fragment antibody scFvP1-1 for expression in plants. The transient expression of scFvP1-1 in tobacco (Nicotiana tabacum) strongly reduced virus accumulation, while transgenic potato (Solanum tuberosum) plants expressing scFvP1-1 showed high levels of resistance following PLRV inoculation by viruliferous aphids. This is the first report that conclusively demonstrates that a PLRV gene product is essential for the completion of the virus life cycle in vivo without genetic alteration of the viral genome. This is also the first time plantibody-mediated resistance has been demonstrated with a luteovirus.
Nickel
Holger
H
Fraunhofer Institute for Molecular Biotechnology and Applied Ecology (IME), Forckenbeckstrasse 6, 52074 Aachen, Germany.
Kawchuk
Lawrence
L
Twyman
Richard M
RM
Zimmermann
Sabine
S
Junghans
Holger
H
Winter
Stephan
S
Fischer
Rainer
R
Prüfer
Dirk
D
D000818:Animals; D000914:Antibodies, Viral / Q000235:genetics / Q000494:pharmacology; D000998:Antiviral Agents / Q000494:pharmacology; D001042:Aphids; D052679:Luteoviridae / Q000502:physiology*; D010935:Plant Diseases / Q000821:virology; D030821:Plants, Genetically Modified; D016601:RNA-Binding Proteins / Q000037:antagonists & inhibitors*; D011198:Solanum tuberosum / Q000821:virology; D014026:Nicotiana / Q000821:virology; D014764:Viral Proteins / Q000037:antagonists & inhibitors*; D014779:Virus Replication
D000914:Antibodies, Viral; D000998:Antiviral Agents; C117690:P1-C25 protein, potato leafroll luteovirus; D016601:RNA-Binding Proteins; D014764:Viral Proteins