Acetonobutylic fermentation: improvement of performances by coupling continuous fermentation and ultrafiltration.
The technology of coupling ultrafiltration and fermentation has been tested with the acetonobutylic fermentation in continuous mode. The device developed was sterilizable by steam and permitted drastic cleaning of the ultrafiltration (UF) membrane without interrupting the continuous fermentation. It has been shown to be an easily operated and reliable experimental tool for studying high-cell-density cultures and inhibition phenomena. With total recycle of biomass, a dry weight concentration of 125 g/L was attained, which greatly enhanced the volumetric solvent productivity of acetonobutylic fermentation in averaging 4. 5 g/L h for significant periods of time (>70 h) and maintaining solvent concentration and yield at acceptable levels.
Département de Génie Biochimique et Alimentaire, UA - CNRS - n degrees 544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077 Toulouse Cédex.
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