[Expression of Bcl-2 and Fas and apoptosis of T lymphocyte in the peripheral blood of patients with end-stage renal diseases].
OBJECTIVE
To investigate the expression of Bcl-2 and Fas and the apoptosis of T lymphocyte in the peripheral blood of patients with end-stage renal diseases; and to test the impact of various dialysis membranes on the apoptosis of T lymphocyte.


METHODS
T lymphocyte was cultured with the stimulation of Phytohemagglutinin (PHA) for 24 h. The apoptosis of T lymphocyte was measured by flow cytometry. The expression of Bcl-2 and Fas was measured with immunohistochemical approach. A total of 10 non-dialyszed (ND) patients, 45 maintenance hemodialysis patients with cellulose acetate (CA) membrane, low-flux (PS-LF) and high-fluxpolusulfone (PS-HF) membrane, and 8 healthy volunteers (HC) participated in the study.


RESULTS
The patients with end-stage renal diseases had greater apoptosis of T lymphocyte than healthy volunteers (P < 0.01). The patients undergoing hemodialysis with CA membrane had greater apoptosis of T lymphocyte than those with PS-LF and PS-HF membranes (P < 0.05). The expression of Bcl-2 in T lymphocyte of patients with end-stage renal diseases was lower than that of healthy valunteers (P < 0. 01). The apoptosis of T lymphocyte was negatively correlated with the expression of Bcl-2 (r = -0. 83, P < 0.01). The expression of Fas in T lymphocyte of patients with end-stage renal diseases was greater than that of healthy valunteers (P < 0.01). The apoptosis of T lymphocyte was positively correlated with the expression of Fas (r = 0.81, P < 0.01).


CONCLUSIONS
Patients with end-stage renal diseases may experience accelerated apoptosis of T lymphocyte, which is associated with the high expression of Fas and low expression of Bcl-2 in T lymphocyte. The apoptosis of T lymphocyte is also influenced by the permeability of the dialysis membranes.
Department of Nephrology, Second People's Hospital of Yibin, Sichuan Province, Yibin 644000, China.
De-Qiong
DQ
Xie
Yi-Ping
YP
Du
Mu
M
Liu
Li
L
Zeng
Hua
H
Gan
Li-Qun
LQ
Chen
D000368:Aged; D017209:Apoptosis; D053222:Fas Ligand Protein / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007676:Kidney Failure, Chronic / Q000097:blood* / Q000473:pathology / Q000628:therapy; D008297:Male; D008875:Middle Aged; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000096:biosynthesis*; D006435:Renal Dialysis; D013601:T-Lymphocytes / Q000378:metabolism* / Q000473:pathology
D053222:Fas Ligand Protein; D019253:Proto-Oncogene Proteins c-bcl-2