Humoral response against Mycobacterium bovis Hsp65 derived fragments in children and young people with various disorders.
Using Western blotting, we investigated IgG antibodies against Mycobacterium bovis heat shock protein 65 (MB-Hsp65) fragments produced by cleavage with cyanogen bromide (CNBr) in 10 healthy controls, 11 patients with juvenile idiopathic arthritis (JIA), and 10 children with various diseases before haematopoietic stem cell transplantation (HSCT). CNBr cleaved MB-Hsp65 to three larger fragments: P1-163, P191-285, and P290-534. Sera of JIA patients and those before HSCT reacted with individual MB-Hsp65 fragments P1-163 and P290-534 significantly more frequently when compared with healthy controls. These results suggested that the key B-cell epitopes of MB-Hsp65 might be located on the aforementioned sequences.
Nguyen
Thi Thu Hien
TT
Department of Molecular Biology and Cell Pathology, Charles University in Prague, Prague, Czech Republic. hien.nguyen@lf3.cuni.cz
Bezouska
Karel
K
Vavrincova
Pavla
P
Sedlacek
Petr
P
Hromadnikova
Ilona
I
D000293:Adolescent; D000328:Adult; D000741:Anemia, Aplastic / Q000276:immunology; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000276:immunology; D001168:Arthritis / Q000276:immunology*; D001171:Arthritis, Juvenile / Q000276:immunology; D001426:Bacterial Proteins / Q000276:immunology*; D015153:Blotting, Western / Q000379:methods*; D018834:Chaperonin 60; D018833:Chaperonins / Q000276:immunology*; D002648:Child; D002675:Child, Preschool; D005260:Female; D006402:Hematologic Diseases / Q000276:immunology*; D018380:Hematopoietic Stem Cell Transplantation; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007223:Infant; D007938:Leukemia / Q000276:immunology*; D008297:Male; D009163:Mycobacterium bovis / Q000276:immunology*
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D018834:Chaperonin 60; D007074:Immunoglobulin G; C074119:heat-shock protein 65, Mycobacterium; D018833:Chaperonins