Excretory-secretory products of larval Fasciola hepatica investigated using a two-dimensional proteomic approach.
So far, very few secreted proteins from trematodes have been characterized, although their role in the mechanisms that allow the parasite to escape host's immune response have been largely documented. Here we performed a proteomic analysis of excretory-secretory proteins from the intra-molluscan larval stages of Fasciola hepatica. We identified two antioxidative enzymes: a Cu/Zn-superoxide dismutase (Cu/Zn SOD) and a thioredoxin (TRX) previously characterized in ES products from adult stages. These results support the importance of parasite detoxication of reactive oxygen species in invertebrate hosts, and raise the question of the possible conservation of major immune evasion effectors across trematode developmental life-stages.
UMR 5244 CNRS-EPHE-UPVD, Parasitologie Fonctionnelle et Evolutive, Université de Perpignan, 52 Ave Paul Alduy, 66860 Perpignan Cedex, France.
Benjamin E F
BE
Gourbal
François
F
Guillou
Guillaume
G
Mitta
Pierre
P
Sibille
André
A
Thèron
Jean-Pierre
JP
Pointier
Christine
C
Coustau
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005210:Fasciola hepatica / Q000737:chemistry*; D007814:Larva / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D013482:Superoxide Dismutase / Q000302:isolation & purification; D013879:Thioredoxins / Q000302:isolation & purification
D020543:Proteome; D013879:Thioredoxins; D013482:Superoxide Dismutase