Preparation of polyclonal antibodies to diminazene and its detection in animal blood plasma.
This work was undertaken to examine the possibility of preparing polyclonal antibodies to diminazene by using colloidal gold nanoparticles as an antigen carrier and adjuvant. The antibodies prepared by us were used for immunodot determination of diminazene in calf-blood plasma. The immunochemical results agreed with those obtained by high-performance liquid chromatographic estimation of diminazene content in animal biological fluids.
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia.
S A
SA
Staroverov
O A
OA
Vasilenko
K P
KP
Gabalov
D V
DV
Pristensky
D N
DN
Yermilov
N M
NM
Aksinenko
S Yu
SY
Shchyogolev
L A
LA
Dykman
D000818:Animals; D000906:Antibodies; D002020:Buffaloes; D004133:Diminazene / Q000032:analysis / Q000097:blood* / Q000276:immunology; D005260:Female; D006046:Gold / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010949:Plasma / Q000737:chemistry; D014344:Trypanocidal Agents / Q000097:blood* / Q000276:immunology
D000906:Antibodies; D014344:Trypanocidal Agents; D006046:Gold; D004133:Diminazene