Lack of association of delta-aminolevulinate dehydratase polymorphisms with blood lead levels and hemoglobin in Romanian women from a lead-contaminated region.
As part of a project on environmental pollution, this study aimed to evaluate associations between blood lead (BPb) levels, hemoglobin (Hb) content, and single-nucleotide polymorphisms (SNPs) of delta-aminolevulinic acid dehydratase (ALAD) gene in 129 unrelated women from Romania. Five SNPs (rs1805313, rs2228083, rs1805312, rs1800435, rs1139488) were analyzed with respect to haplotype structure and impact on BPb levels and Hb content with proportional odds and analysis of covariance models. Combinations of SNPs were rare (16%). Low haplotype diversity was found with seven haplotypes. One rare haplotype implied the C allele of rs1800435, often referred to as the ALAD2 allele (frequency 8.6%). The putative risk genotype (CC) occurred in only one woman with BPb below 0.5 microg/dl. Median BPb was 4.8 microg/dl and differed markedly by community with a level of 12.5 microg/dl near a mining-spill region. Hb was regular (interquartile range 12.3-13.7 g/dl) and not correlated with BPb, although quantitatively lower in women living near the spill region. No significant associations were found for BPb or Hb with SNPs, haplotypes, or diplotypes. BPb levels were higher in this region than in populations from industrialized countries but without hematotoxic effects. An impact of ALAD2 on BPb or Hb was not seen in these women.
BGFA-Research Institute of Occupational Medicine, German Social Accident Insurance, Ruhr-Universität Bochum, Bochum, Germany. rabstein@bgfa.de
Sylvia
S
Rabstein
Klaus
K
Unfried
Ulrich
U
Ranft
Thomas
T
Illig
Melanie
M
Kolz
Chinara
C
Mambetova
Mariana
M
Vlad
Cecilia
C
Roman
Tobias
T
Weiss
Doreen
D
Becker
Thomas
T
Brüning
Beate
B
Pesch
D000328:Adult; D000368:Aged; D004781:Environmental Exposure; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000097:blood*; D005260:Female; D005838:Genotype; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D007854:Lead / Q000097:blood*; D008875:Middle Aged; D008906:Mining; D020641:Polymorphism, Single Nucleotide; D000623:Porphobilinogen Synthase / Q000235:genetics*; D012383:Romania
D004785:Environmental Pollutants; D006454:Hemoglobins; D007854:Lead; D000623:Porphobilinogen Synthase