Phylogenetic analysis of Porphyromonas species isolated from the oral cavity of Australian marsupials.
Porphyromonas species are frequently isolated from the oral cavity and are associated with periodontal disease in both animals and humans. Black, pigmented Porphyromonas spp. isolated from the gingival margins of selected wild and captive Australian marsupials with varying degrees of periodontal disease (brushtail possums, koalas and macropods) were compared phylogenetically to Porphyromonas strains from non-marsupials (bear, wolf, coyote, cats and dogs) and Porphyromonas gingivalis strains from humans using 16S rRNA gene sequence analysis. The results of the phylogenetic analysis identified three distinct groups of strains. A monophyletic P. gingivalis group (Group 1) contained only strains isolated from humans and a Porphyromonas gulae group (Group 2) was divided into three distinct subclades, each containing both marsupial and non-marsupial strains. Group 3, which contained only marsupial strains, including all six strains isolated from captive koalas, was genetically distinct from P. gulae and may constitute a new Porphyromonas species.
Mikkelsen
Deirdre
D
Oral Biology and Pathology, School of Dentistry, Faculty of Health Sciences, The University of Queensland, St Lucia, 4072, Queensland, Australia.
Milinovich
Gabriel J
GJ
Burrell
Paul C
PC
Huynh
Sharnan C
SC
Pettett
Lyndall M
LM
Blackall
Linda L
LL
Trott
Darren J
DJ
Bird
Philip S
PS
D000818:Animals; D001315:Australia; D001424:Bacterial Infections / Q000382:microbiology / Q000662:veterinary; D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D020459:Genes, rRNA; D006801:Humans; D008394:Marsupialia / Q000382:microbiology*; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D010510:Periodontal Diseases / Q000382:microbiology / Q000662:veterinary; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D017861:Porphyromonas / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S