An enzyme electrode for specific determination of L-lysine: A real-time control sensor.
Lysine decarboxylase (L-lysine carboxylyase, E.C.4.1.1.18) is immobolized on a carbon dioxide gas-sensing electrode, by copolymerization with gelatin using the bifuncitional agent glutaraldehyde. The enzyme electrodes thus prepared are used in a continuous flow system to measure the concentration of L-lysine in a mixture of amino acids. The measuring time for each sample is about 3 min, including response and rinsing times. The electrode response is linear between 0.01-1 g/L and has a high specificity for L-lysine. The enzyme electrode response to lysine at concentrations below 0.5 g/L is stable on repeated use for at least 500 assays.
Laboratoire de Technologie Enzymatique, E.R.A. No 338 du C.N.R.S., Universite de Technologie de Compiègne, B.P. 233, 60206 Compiègne, France.
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ND
Tran
J L
JL
Romette
D
D
Thomas