Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis.
During brown planthopper (BPH) feeding on rice plants, we employed a modified representational difference analysis (RDA) method to detect rare transcripts among those differentially expressed in SNBC61, a BPH resistant near-isogenic line (NIL) carrying the Bph1 resistance gene. This identified 3 RDA clones: OsBphi237, OsBphi252 and OsBphi262. DNA gel-blot analysis revealed that the loci of the RDA clones in SNBC61 corresponded to the alleles of the BPH resistant donor Samgangbyeo. Expression analysis indicated that the RDA genes were up-regulated in SNBC61 during BPH feeding. Interestingly, analysis of 64 SNBC NILs, derived from backcrosses of Samgangbyeo with a BPH susceptible Nagdongbyeo, using a cleaved amplified polymorphic sequence (CAPS) marker indicated that OsBphi252, which encodes a putative lipoxygenase (LOX), co-segregates with BPH resistance. Our results suggest that OsBphi252 is tightly linked to Bph1, and may be useful in marker-assisted selection (MAS) for resistance to BPH.
Rice Division, Yeongnam Agricultural Research Institute, National Institute of Crop Science, RDA, Milyang, South Korea.
Dong-Soo
DS
Park
Min-Young
MY
Song
Soo-Kwon
SK
Park
Sang-Kyu
SK
Lee
Jong-Hee
JH
Lee
Song-Yi
SY
Song
Moo Young
MY
Eun
Tae-Ryong
TR
Hahn
Jae-Keun
JK
Sohn
Gihwan
G
Yi
Min-Hee
MH
Nam
Jong-Seong
JS
Jeon
D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D032461:Chromosomes, Plant / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics; D015870:Gene Expression; D017343:Genes, Plant; D006430:Hemiptera / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions / Q000235:genetics; D012275:Oryza / Q000235:genetics* / Q000469:parasitology*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000469:parasitology
D018744:DNA, Plant