Fluorescence endomicroscopy with structured illumination.
We present an endomicroscope apparatus that utilizes structured illumination to produce high resolution (approximately 2.6 microm) optically sectioned fluorescence images over a field of view of about 240 microm. The endomicroscope is based on the use of a flexible imaging fiber bundle with a miniaturized objective. We also present a strategy to largely suppress structured illumination artifacts that arise when imaging in thick tissue that exhibits significant out-of-focus background. To establish the potential of our endomicroscope for preclinical or clinical applications, we provide images of BCECF-AM labeled rat colonic mucosa.
Department of Biomedical Engineering, Boston University, 44 Cummington St., Boston, MA 02215, USA. nesa@bu.edu
Nenad
N
Bozinovic
Cathie
C
Ventalon
Tim
T
Ford
Jerome
J
Mertz
D000818:Animals; D003106:Colon / Q000166:cytology*; D004724:Endoscopy; D007089:Image Enhancement / Q000295:instrumentation*; D007413:Intestinal Mucosa / Q000166:cytology*; D008029:Lighting / Q000295:instrumentation*; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D019047:Phantoms, Imaging; D051381:Rats