Androgen disruption and toxicity tests of Butea superba Roxb., a traditional herb used for treatment of erectile dysfunction, in male rats.
OBJECTIVE
To evaluate the effects of the tuberous powder of Butea superba Roxb. (Leguminosae) on blood testosterone and luteinizing hormone (LH), and toxicity in male rats.


METHODS
Adult male Wistar rats were orally treated with 0, 10, 100, 150 or 200 mg/kg BW/day of B. superba powder suspension in 0.7 ml distilled water for 90 consecutive days. Blood samples were collected every 30 days and submitted to testosterone and LH analysis. On the 90th day of treatment, blood and the main organs were collected for haematological and histopathological analysis, respectively.


RESULTS
The adverse effects found included an increase in spleen relative weight, and increased serum level of alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in rats treated with 150 mg/kg BW/day B. superba powder. At 200 mg/kg BW/day treatment, rats showed significant decreased and increased blood levels of neutrophil and eosinophil, respectively, and a decrease in serum creatinine levels. Serum hormonal analysis revealed a dose-dependent decrease in testosterone, but not LH, in rats treated with 150 and 200 mg/kg BW/day B. superba powder.


CONCLUSIONS
Subchronic treatment of B. superba tuberous powder suspension at high doses in male rats exhibited adverse effects to blood chemistry, haematology, and blood testosterone level. The results of the study should initiate awareness of the possible adverse risk of over-dose consumption of B. superba products for treatment of erectile dysfunction (ED) in mature males.
Cherdshewasart
Wichai
W
Department of Biology, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok, Thailand. cwichai@sc.chula.ac.th
Bhuntaku
Papong
P
Panriansaen
Rattana
R
Dahlan
Winai
W
Malaivijitnond
Suchinda
S
D000818:Animals; D031290:Butea / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D007172:Erectile Dysfunction / Q000097:blood / Q000188:drug therapy*; D007986:Luteinizing Hormone / Q000097:blood*; D008297:Male; D008517:Phytotherapy / Q000009:adverse effects*; D028321:Plant Preparations / Q000009:adverse effects*; D035281:Plant Tubers / Q000009:adverse effects; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000097:blood*
D028321:Plant Preparations; D013739:Testosterone; D007986:Luteinizing Hormone