Isolation and partial characterization of a mutant of Penicillium funiculosum for the saccharification of straw.
Clearing of agar plates containing ball-milled, delignified straw has been used for screening mutants of Penicillium funiculosum IMI 87160 iii. The effects of glycerol and a number of sugars on the clearing were investigated for selecting derepressed mutants. The beta-glucosidase synthesis by one such mutant, C22c, in shake flasks containing straw was not repressed by 5% glycerol, whereas activities on filter paper, CM-cellulose, and p-nitrophenyl-beta-xylosidase were only partially derepressed; xylanase was extensively derepressed. The evidence for separate control of the enzymes involved in the solubilization of straw is discussed.
Department of Microbial Biochemistry, Rowett Research Institute, Bucksburn, Aberdeen, AB2 9SB, Scotland.
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Hoffman
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Wood