Fluorescence enhancement effect of morin-nucleic acid-L-cysteine-capped nano-ZnS system and the determination of nucleic acid.
It is found that L-cysteine-capped nano-ZnS can further enhance the fluorescence intensity of the morin-nucleic acid system. Under optimum conditions, the enhanced intensity of fluorescence is proportional to the concentration of nucleic acid in the range of 7.0 x 10(-8)-1.0 x 10(-5) g mL(-1) for fish sperm DNA (fsDNA) and 9.0 x 10(-8)-5.0 x 10(-6) g mL(-1) for yeast RNA (yRNA). The corresponding detection limits (S/N = 3) are 2.0 x 10(-8) g mL(-1) and 4.0 x 10(-8) g mL(-1), respectively. The interaction mechanisms of morin-nucleic acid-L-cysteine-capped nano-ZnS system are studied by multiple techniques. It is considered that there exists synergistic effects of groove binding and electrostatic interaction between morin, L-cysteine-capped nano-ZnS and nucleic acid, and the complex of morin-L-cysteine-capped nano-ZnS-nucleic acid is formed.
Zhao
Liangliang
L
A Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, Shandong, PR China.
Wu
Xia
X
Ding
Honghong
H
Yang
Jinghe
J
D000818:Animals; D003545:Cysteine; D005419:Flavonoids; D006863:Hydrogen-Ion Concentration; D008297:Male; D049329:Nanostructures; D009696:Nucleic Acids / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods; D013094:Spermatozoa / Q000648:ultrastructure; D013440:Sulfides; D017967:Zinc Compounds; D047309:Flavones
D003545:Cysteine; D005419:Flavonoids; D009696:Nucleic Acids; D013440:Sulfides; D017967:Zinc Compounds; C008548:morin; D047309:Flavones; C031238:zinc sulfide