Preliminary study of embryo development following assessment of male and female gametes.
Gamete characteristics are likely to contribute to embryo development. In this preliminary study, early parameters were assessed in embryos generated following microinjection of hyaluronan-bound and unbound spermatozoa into 176 sibling oocytes, which had themselves been assessed using a polarizing light microscope imaging system. The fertilization and early cleavage rates, and day 3 embryo quality did not differ when oocytes displaying similar characteristics were inseminated with either bound or unbound sperm. Regardless of the binding characteristics of the sperm, early embryos displaying good and poor quality derived from oocytes displaying visible spindles and similar characteristics. These results indicate that early embryo developmental characteristics were independent from the hyaluronic acid binding capacity of the sperm when oocytes displayed a visible spindle.
Assisted Conception Unit, German Hospital Istanbul and Bahçeci Women Health Care Centre, Siraselviler Cd. 119, 80860, Istanbul, Turkey. nadirc@superonline.com
H Nadir
HN
Ciray
Onder
O
Coban
Asina
A
Bayram
Ali
A
Kizilkanat
Mustafa
M
Bahçeci
D000328:Adult; D047108:Embryonic Development; D005260:Female; D005307:Fertilization in Vitro; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism; D008297:Male; D008859:Microscopy, Polarization; D009865:Oocytes / Q000166:cytology*; D010865:Pilot Projects; D011247:Pregnancy; D013094:Spermatozoa / Q000166:cytology* / Q000378:metabolism
D006820:Hyaluronic Acid