Optical microscopic observation of fluorescence enhanced by grating-coupled surface plasmon resonance.
On the substrate carrying a sub-wavelength grating covered with a thin metal layer, a fluorescent dye-labeled cell was observed by fluorescence microscope. The fluorescence intensity was more than 20 times greater than that on an optically flat glass substrate. Such a great fluorescence enhancement from labeled cells bound to the grating substrate was due to the excitation by grating coupled surface plasmon resonance. The application of a grating substrate to two-dimensional detection and fluorescence microscopy appears to offer a promising method of taking highly sensitive fluorescence images.
Tawa
Keiko
K
Research Institute for Cell Engineering, AIST, Ikeda, Osaka 563-8577, Japan. tawa-keiko@aist.go.jp
Hori
Hironobu
H
Kintaka
Kenji
K
Kiyosue
Kazuyuki
K
Tatsu
Yoshiro
Y
Nishii
Junji
J
D003198:Computer Simulation; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007089:Image Enhancement / Q000295:instrumentation*; D008027:Light; D008856:Microscopy, Fluorescence / Q000295:instrumentation*; D008962:Models, Theoretical; D012031:Refractometry / Q000295:instrumentation*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D020349:Surface Plasmon Resonance / Q000295:instrumentation*