Melatonin exerts a more potent effect than S-adenosyl-l-methionine against iron metabolism disturbances, oxidative stress and tissue injury induced by obstructive jaundice in rats.
Melatonin and S-adenosyl-l-methionine (SAMe) prevent oxidative stress and tissue dysfunction in obstructive jaundice (OJ). Lipid peroxidation is exacerbated in the presence of trace amounts of iron (Fe). The study investigated the regulation by melatonin and SAMe the induction of oxidative stress, iron metabolism disturbances and tissue injury in an experimental model of OJ. Different parameters of lipid peroxidation, antioxidant status, tissue injury and Fe metabolism were determined in liver and blood. OJ induced Fe accumulation in liver, and increased transferrin (Tf) saturation and loosely bound Fe content in blood. Melatonin, and SAMe at lesser extent, enhanced protein Tf content in liver and blood, that reduced loosely bound Fe content in blood. Melatonin and SAMe did not affect ferritin (FT) and Tf mRNA expression, but reduced Tf receptor (TfR) mRNA expression in liver. In conclusion, the effect of melatonin and SAMe on Fe metabolism may be included in the beneficial properties of these agents on lipid peroxidation and tissue injury induced by OJ.
Muñoz-Castañeda
Juan R
JR
Liver Research Unit, Reina Sofía University Hospital, Córdoba, Spain.
Túnez
Isaac
I
Herencia
Carmen
C
Ranchal
Isidora
I
González
Raúl
R
Ramírez
Luz M
LM
Arjona
Alvaro
A
Barcos
Montserrat
M
Espejo
Isabel
I
Cruz
Adolfo
A
Montilla
Pedro
P
Padillo
Francisco J
FJ
Muntané
Jordi
J
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D004195:Disease Models, Animal; D005293:Ferritins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D041781:Jaundice, Obstructive / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011990:Receptors, Transferrin / Q000235:genetics; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D014168:Transferrin / Q000235:genetics / Q000378:metabolism*
D000975:Antioxidants; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D014168:Transferrin; D012436:S-Adenosylmethionine; D005293:Ferritins; D008550:Melatonin