The polymorphism of HLA-DR and -DQ allelic genes associated with intrahepatic cholestasis of pregnancy.
OBJECTIVE
This study was conducted to investigate the association of the polymorphisms of human leucocyte antigen (HLA) class II DRB1 and DQB1 alleles and the risk for intrahepatic cholestasis of pregnancy (ICP).


METHODS
Genomic DNA was extracted from peripheral venous blood leucocytes in 42 ICP patients and 38 normal controls and from their corresponding neonates. HLA class II DRB1 and DQB1 genotypes were determined using sequence-specific primer polymerase chain reaction (SSP-PCR).


RESULTS
Genotype analyses of HLA class revealed a significantly increased frequency of HLA-DR11 allele in ICP patients when compared with the controls (16.67% vs. 3.95%, p < 0.01), but no significant difference in other genotypes. Interestingly, analysis of the fetomaternal's -DR and -DQ alleles showed no significant difference between the patient and control groups.


CONCLUSIONS
These results indicate that HLA-DR11 allele is associated with the susceptibility of ICP in the Chinese Changsha area.
Department of Obstetrics and Gynecology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China. dylcsu@yahoo.com.cn
Yiling
Y
Ding
Hong
H
Shen
Xin
X
Wang
Xuemei
X
Fan
Xiang
X
Wu
Xiao
X
Yang
D000328:Adult; D000483:Alleles; D002681:China; D002780:Cholestasis, Intrahepatic / Q000235:genetics*; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006683:HLA-DQ Antigens / Q000235:genetics*; D059866:HLA-DQ beta-Chains; D006684:HLA-DR Antigens / Q000235:genetics*; D059810:HLA-DR Serological Subtypes; D059811:HLA-DRB1 Chains; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature; D007235:Infant, Premature, Diseases / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011248:Pregnancy Complications / Q000235:genetics*
D006683:HLA-DQ Antigens; D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D006684:HLA-DR Antigens; D059810:HLA-DR Serological Subtypes; C080766:HLA-DR11 antigen; D059811:HLA-DRB1 Chains