[Genome analysis of a newly isolated enterovirus].
OBJECTIVE
To demonstrate molecular characterization of a newly isolated enterovirus.


METHODS
Virus were isolated from patient with unknown-causing disease and tested by reverse transcription-polymerase chain reaction (RT-PCR) and 5'3'RACE (rapid amplification of cDNA ends, RACE), in an attempt to obtain the sequence of this newly isolated enterovirus.


RESULTS
Sequence analysis showed that this newly isolated enterovirus shared 83%-94% nucleotide identity and 91%-100% amino acid identity with enterovirus 89. Phylogenetic analysis indicated that it was probably a new subtype of enterovirus 89.


CONCLUSIONS
This newly isolated enterovirus in the stool specimen from patient has the same serotype with enterovirus 89, but it was probably a new subtype of enterovirus 89.
Department of Virology, Institute of Infectious Diseases, No. 302 Hospital, Beijing, China.
Jun
J
Hou
Yan
Y
Hu
Hong-hui
HH
Sheng
Bing-ke
BK
Bai
Zhi-jie
ZJ
Wang
Pan-yong
PY
Mao
D000595:Amino Acid Sequence; D001483:Base Sequence; D003001:Cloning, Molecular; D004770:Enterovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D005243:Feces / Q000821:virology; D016679:Genome, Viral / Q000235:genetics*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA
D012367:RNA, Viral