Affinity purification of egg yolk immunoglobulins (IgY) with a stable synthetic ligand.
Chicken IgY (egg yolk immunoglobulin) is a functional equivalent of mammalian IgG. Traditional methods for IgY purification involve multi-step procedures that result in low recovery of IgY. After a large scale screening of our 700-member synthetic ligand library synthesized by epichlorohydrin and cyanuric chloride methods, a high efficiency ligand of IgY was found. By one-step purification with this ligand, the purity of IgY could reach 92.1%, and the recovery of IgY could reach 78.2%. This synthetic ligand had a higher binding capacity of 74.8 mg IgY/ml and had no negative effects on immunoreactivity. Remarkably, this ligand was also highly stable and could resist 1M NaOH, thus having great potential for the industrial-scale production of IgY.
Dong
Dexian
D
MOE Key Laboratory of Microbial Metabolism, College of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Liu
Haoran
H
Xiao
Qishi
Q
Li
Rongxiu
R
D000818:Animals; D000906:Antibodies / Q000302:isolation & purification / Q000378:metabolism; D002417:Cattle; D002645:Chickens; D002846:Chromatography, Affinity / Q000379:methods*; D010856:Columbidae; D004372:Ducks; D004530:Egg Yolk / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007136:Immunoglobulins / Q000302:isolation & purification* / Q000378:metabolism; D008024:Ligands; D011485:Protein Binding; D011784:Quail; D011817:Rabbits; D054852:Small Molecule Libraries / Q000138:chemical synthesis / Q000737:chemistry* / Q000378:metabolism
D000906:Antibodies; C016512:IgY; D007136:Immunoglobulins; D008024:Ligands; D054852:Small Molecule Libraries