Kinetic characterization of the extracellular xylanases of Thermomonospora sp.
This study deals with characterizing the extracellular xylanases produced by a strain of the thermophilic bacterial genus Thermomonospora. Supernatant from centrifuged fermentation broth was used as a crude enzyme preparation. From pH 5.5 to pH 7.7 the temperature optimum based on a 10-min assay of activity was 80 degrees C. The crude enzyme had a half-life of approximately 1 month when stored at 55 degrees C at pH 6.5. The enzyme produced a mixture of xylose oligomers from xylan, with xylobiose occuring in greatest quantity on a molar basis. Only trace quantities of xylose were produced by this hydrolysis.
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Department of Chemical Engineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104.
Humphrey
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