Cloning and heterologous expression of Cryptococcus neoformans CnSRB1 cDNA in Saccharomyces cerevisiae.
In this study, we report the results of cloning, sequencing and functional analysis by complementation test of the putative Cryptococcus neoformans homolog CnSRB1. The nucleotide sequence revealed 63% identity, and the deduced amino acid sequence showed 66 and 64% identity to its respective homolog of Saccharomyces cerevisiae and Candida albicans, respectively. Functional complementation test indicated that the putative CnSRB1 gene could compensate the defect caused by a mutation in ScSRB1 in the S. cerevisiae srb1 mutant. Taken together, these results suggest that the putative CnSrblp is a functional homolog of ScSrb1p.
National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phahonyothin Road, Klong Luang, Pathum Thani 12120, Thailand. saradee@biotec.or.th
Saradee
S
Warit
Lubomira I
LI
Stateva
Prasit
P
Palittapongarnpim
D015152:Blotting, Northern; D015139:Blotting, Southern; D002176:Candida albicans / Q000201:enzymology / Q000235:genetics; D003001:Cloning, Molecular; D003455:Cryptococcus neoformans / Q000201:enzymology / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D009713:Nucleotidyltransferases / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D012689:Sequence Homology, Nucleic Acid
D018076:DNA, Complementary; D005656:Fungal Proteins; D009713:Nucleotidyltransferases; C026917:mannose 1-phosphate guanylyltransferase