Structure of the recombinant antibody Fab fragment f3p4.
The structure of the antibody Fab fragment f3p4, which was selected from a subset of the synthetic HuCAL antibody library to bind the sodium citrate symporter CitS, is described at 1.92 A resolution. Comparison with computational models revealed deviations in a few framework positions and in the binding loops. The crystals belong to space group P2(1)2(1)2 and contain four molecules in the asymmetric unit, with unit-cell parameters a=102.77, b=185.92, c=102.97 A. These particular unit-cell parameters allowed pseudo-merohedral twinning; interestingly, the twinning law relates a twofold screw axis to a twofold axis.
Frey
Daniel
D
Department of Biochemistry, University of Zürich, Switzerland.
Huber
Thomas
T
Plückthun
Andreas
A
Grütter
Markus G
MG
D001666:Binding Sites, Antibody; D022801:Complementarity Determining Regions / Q000737:chemistry / Q000235:genetics; D018360:Crystallography, X-Ray; D006801:Humans; D007140:Immunoglobulin Fab Fragments / Q000737:chemistry* / Q000235:genetics; D008958:Models, Molecular; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D055672:Static Electricity
D022801:Complementarity Determining Regions; D007140:Immunoglobulin Fab Fragments; D011994:Recombinant Proteins