Melatonin exerts a more potent effect than S-adenosyl-l-methionine against iron metabolism disturbances, oxidative stress and tissue injury induced by obstructive jaundice in rats.
Melatonin and S-adenosyl-l-methionine (SAMe) prevent oxidative stress and tissue dysfunction in obstructive jaundice (OJ). Lipid peroxidation is exacerbated in the presence of trace amounts of iron (Fe). The study investigated the regulation by melatonin and SAMe the induction of oxidative stress, iron metabolism disturbances and tissue injury in an experimental model of OJ. Different parameters of lipid peroxidation, antioxidant status, tissue injury and Fe metabolism were determined in liver and blood. OJ induced Fe accumulation in liver, and increased transferrin (Tf) saturation and loosely bound Fe content in blood. Melatonin, and SAMe at lesser extent, enhanced protein Tf content in liver and blood, that reduced loosely bound Fe content in blood. Melatonin and SAMe did not affect ferritin (FT) and Tf mRNA expression, but reduced Tf receptor (TfR) mRNA expression in liver. In conclusion, the effect of melatonin and SAMe on Fe metabolism may be included in the beneficial properties of these agents on lipid peroxidation and tissue injury induced by OJ.
Liver Research Unit, Reina Sofía University Hospital, Córdoba, Spain.
Juan R
JR
Muñoz-Castañeda
Isaac
I
Túnez
Carmen
C
Herencia
Isidora
I
Ranchal
Raúl
R
González
Luz M
LM
Ramírez
Alvaro
A
Arjona
Montserrat
M
Barcos
Isabel
I
Espejo
Adolfo
A
Cruz
Pedro
P
Montilla
Francisco J
FJ
Padillo
Jordi
J
Muntané
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D004195:Disease Models, Animal; D005293:Ferritins / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D041781:Jaundice, Obstructive / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008550:Melatonin / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011990:Receptors, Transferrin / Q000235:genetics; D012436:S-Adenosylmethionine / Q000494:pharmacology*; D014168:Transferrin / Q000235:genetics / Q000378:metabolism*
D000975:Antioxidants; D012333:RNA, Messenger; D011990:Receptors, Transferrin; D014168:Transferrin; D012436:S-Adenosylmethionine; D005293:Ferritins; D008550:Melatonin