Paramagnetic nanoparticle-based detection of hepatitis B virus using cathodic stripping voltammetry.
A nanoparticle-based electrochemical method for detection of hepatitis B virus DNA sequences has been developed. This method relies on the adsorption of amplified hepatitis B virus DNA strands on to probe-coated paramagnetic particles and electrochemical detection of hybridized strands using a hanging mercury drop electrode. For hepatitis B virus detection, a combination of dynamic DNA hybridization on transportable reactive surfaces and label-free detection of DNA based on the electrochemical determination of adenines was used. Separation of the hybridization area from the detection electrode eliminates non-specific adsorption of long DNAs, and combining this method with dynamic synthesis of probe may result in new flexible approaches for detection of other infectious agents by lab-on-a-chip technology.
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Kamal
K
Fatemi
Hedayatollah
H
Ghourchian
Abed-Ali
AA
Ziaee
Shahram
S
Samiei
Hana
H
Hanaee
D000225:Adenine / Q000737:chemistry; D017931:DNA Primers / Q000378:metabolism; D004279:DNA, Viral / Q000302:isolation & purification; D055664:Electrochemical Techniques; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D008280:Magnetics; D053768:Metal Nanoparticles / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity
D017931:DNA Primers; D004279:DNA, Viral; D000225:Adenine