A well-defined diblock copolymer of poly-(ethylene oxide)-block-poly (4-vinylpyridine) for separation of basic proteins by capillary zone electrophoresis.
A series of well-defined diblock copolymers, poly(ethylene oxide)-block-poly(4-vinylpyridine) (PEO-b-P4VP) used as physical coating of capillaries, were synthesized by atom transfer radical polymerization (ATRP). EOF measurement results showed that all synthesized PEO-b-P4VP diblock copolymer-coated capillaries in this report could suppress EOF effectively compared to the bare fused-silica capillaries, and efficient separations of basic proteins were achieved. The effects of the molecular weight of P4VP block in PEO-b-P4VP and buffer pH on the separation of basic proteins for CE were investigated in detail. Moreover, the relationships between morphologies of PEO-b-P4VP diblock copolymers in buffer, which were studied by transmission electron microscopy, and the separation efficiencies of basic protein with PEO-b-P4VP diblock copolymers coatings were discussed.
Liu
Hang
H
Department of Polymer Science and Engineering, Joint Laboratory of Polymer Thin Films and Solution, University of Science and Technology of China, Hefei, P. R. China.
Shi
Ronghua
R
Wan
Wenming
W
Yang
Runmiao
R
Wang
Yanmei
Y
D001798:Blood Proteins / Q000302:isolation & purification; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D046529:Microscopy, Electron, Transmission; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D015203:Reproducibility of Results; D014753:Vinyl Compounds / Q000138:chemical synthesis / Q000737:chemistry*
D001798:Blood Proteins; D014753:Vinyl Compounds; C520176:poly(4-vinylpyridine)-block-poly(ethylene oxide); D011092:Polyethylene Glycols