The influence of polymorphisms of glutathione S-transferases M1 and M3 on the development of human urothelial cancer.
Cigarette smoking is the most important risk factor for development of transitional cell carcinoma of the urinary bladder. The effect of polymorphisms of glutathione S-transferases M1 (GSTM1) and M3 (GSTM3) on the influence of cigarette smoking on urinary bladder carcinogenesis was investigated. In total, 293 bladder cancer patients from hospitals in Dortmund and Wittenberg as well as 176 patients without any malignancy from a Department of Surgery from Dortmund were genotyped for GSTM1 and GSTM3 according to standard PCR/RFLP methods. Smoking habits were quantified by a standardized interview. The proportion of GSTM1 negative cases was 63% in the entire bladder cancer cases group compared to 50% in controls. The GSTM3*A/*A genotype was 76% in cancer cases versus 74% in controls. Smokers and ex-smokers were overrepresented in bladder cancer cases. A significant association between smoking status and GSTM1 or GSTM3 genotype was not detected. The elevated proportion of GSTM1 negative bladder cancer cases shows an effect of this polymorphic enzyme on development of bladder cancer. In contrast to other studies, an influence of GSTM1 on the risk due to cigarette smoking was not observed.
Leibniz Research Centre for Working Environment and Human Factors, Institut fur Arbeitsphysiologie an der Universitat Dortmund (IfADo), Dortmund, Germany.
Klaus
K
Golka
Tobias
T
Schmidt
Thilo
T
Seidel
Holger
H
Dietrich
Hermann C
HC
Roemer
Dietrich
D
Lohlein
Thomas
T
Reckwitz
Jurgen
J
Sokeland
Wobbeke
W
Weistenhofer
Meinolf
M
Blaszkewicz
Silvia
S
Selinski
D000368:Aged; D002295:Carcinoma, Transitional Cell / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics; D016022:Case-Control Studies; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D020022:Genetic Predisposition to Disease; D005838:Genotype; D005982:Glutathione Transferase / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D011110:Polymorphism, Genetic; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D001749:Urinary Bladder Neoplasms / Q000139:chemically induced / Q000201:enzymology* / Q000235:genetics
D005982:Glutathione Transferase; C483009:GSTM3 protein, human