Evaluation of genetic damage in a Brazilian population occupationally exposed to pesticides and its correlation with polymorphisms in metabolizing genes.
Cytogenetic damage in individuals occupationally exposed to pesticides has received the attention of investigators in several countries, but no definitive conclusions can yet be made. The present study aimed at assessing if prolonged exposure to complex mixtures of pesticides leads to an increase in cytogenetic damage. Vineyard workers exposed to pesticides in Caxias do Sul (Brazil) were evaluated using the micronucleus (MN) test in binucleated lymphocytes and the comet assay in peripheral leukocytes. In order to evaluate if genetically determined individual variations in xenobiotic metabolizing capacity could modify individual susceptibility to the possible genotoxic effects of pesticides, the subjects were genotyped for several genes: GSTT1, GSTM1, GSTP1, CYP1A1, CYP2E1 and PON. The study involved a total number of 173 men: 108 were agricultural workers exposed to pesticides and 65 were controls. The present study showed a high rate of MN and DNA damage in pesticide-exposed individuals (P <or= 0.001; Mann-Whitney U-test). In addition, some effects of genetic polymorphisms in PON in the modulation of MN results were observed in the exposed group, and an association between GSTM1, GSTT1 and CYP2E1 polymorphisms was suggested.
da Silva
Juliana
J
Laboratório de Genética Toxicológica, PPGGTA e PPGECIM, Universidade Luterana do Brasil, Canoas-RS, Brazil.
Moraes
Camila R
CR
Heuser
Vanina D
VD
Andrade
Vanessa M
VM
Silva
Fernanda R
FR
Kvitko
Kátia
K
Emmel
Vanessa
V
Rohr
Paula
P
Bordin
Diana Lilian
DL
Andreazza
Ana Cristina
AC
Salvador
Mirian
M
Henriques
João A P
JA
Erdtmann
Bernardo
B
D000328:Adult; D000383:Agriculture; D043303:Aryldialkylphosphatase / Q000235:genetics; D001938:Brazil; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics; D004249:DNA Damage / Q000235:genetics*; D004798:Enzymes / Q000235:genetics*; D051549:Glutathione S-Transferase pi / Q000235:genetics; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D008297:Male; D015162:Micronucleus Tests; D008875:Middle Aged; D016273:Occupational Exposure; D010575:Pesticides / Q000633:toxicity*; D011110:Polymorphism, Genetic
D043303:Aryldialkylphosphatase; D019363:Cytochrome P-450 CYP1A1; D019392:Cytochrome P-450 CYP2E1; D004798:Enzymes; D051549:Glutathione S-Transferase pi; D005982:Glutathione Transferase; D010575:Pesticides; C413545:glutathione S-transferase T1; C496556:GSTP1 protein, human; C468406:PON1 protein, human