[Characterization of the cytopathic effect in human bronchial epithelial cell after Human Bocavirus Infection (HBoV)].
OBJECTIVE
In this study, human bronchial epithelial cells were inoculated with positive sputum specimens of HBoV. After four days' infection, cytopathic effects (CPE) were observed by inverted microscopy. These viruses all cause typical cell damages such as rounded and shrivelled, fusion and fallout. These damages got quick following increased future degenerations. The other assay result of CPE within the infected cells were observed by inverted microscopy, have typical "owl's eye" plaque and above 90 percent hemadsorption within the infected cells by erythrocytes for hemadsorption technique. The typical fluorescence lump of nucleus within the infected cells was found by indirect immunofluorescence technique.


CONCLUSIONS
Isolation and identification of HBoV could be done in the human bronchial epithelial cell, and we found some characterizing CPE in the human bronchial epithelial cell after HBoV infection. The above studies pave a way for studying pathogenicity of human bocavirus.
Wenling Affiliated Hospital, Wenzhou Medical College, Zhejiang, China.
Feng
F
Lin
Jian-yi
JY
Hou
Min-qiao
MQ
Zheng
Feng
F
Wu
Ai-ping
AP
Zeng
Hua
H
Li
Chang-hua
CH
Zheng
Hong
H
Chen
Xu-yang
XY
Li
Gao-feng
GF
Rao
Yi-han
YH
Mo
En-pei
EP
Huang
D052660:Bocavirus / Q000502:physiology*; D001980:Bronchi / Q000166:cytology; D016923:Cell Death / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000821:virology*; D019084:Fluorescent Antibody Technique, Indirect; D054884:Host-Pathogen Interactions; D006801:Humans; D008856:Microscopy, Fluorescence