The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells.
We investigated the effect of a triterpene saponoside from Lysimachia thyrsiflora L. upon the viability, proliferation, morphology and cell motility of human melanoma HTB-140 cells and human skin fibroblasts (HSFs). The compound, denoted LTS-4, decreased the viability and rate of cell growth of both cell types in a time-and dose-dependent manner, and proved cytotoxic against cancer cells at significantly lower concentrations than for fibroblasts. LTS-4 also affected the morphology of the examined cells, causing vacuolisation and actin cytoskeleton disintegration, and had an inhibitory effect on the tumour cell motility.
Department of Pharmacognosy, Faculty of Pharmacy, Jagiellonian University, ul. Medyczna 9, 30-688, Kraków, Poland. mfgalant@cyf-kr.edu.pl
Agnieszka
A
Galanty
Marta
M
Michalik
Lukasz
L
Sedek
Irma
I
Podolak
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008545:Melanoma / Q000378:metabolism*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D029623:Primulaceae / Q000737:chemistry; D012503:Saponins / Q000494:pharmacology*; D012867:Skin / Q000166:cytology*
C524508:3-O-(beta-D-xylopyranosyl-(1-2)-beta-D-glucopyranosyl-(1-4)-(beta-D-glucopyranosyl-(1-2))-alpha-L-arabinopyranosyl)cyclamiretin A; D012503:Saponins; D009828:Oleanolic Acid