Expression of oxygen-regulated protein 150 (ORP150) in skin wound healing and its application for wound age determination.
Immunohistochemical study combined with morphometry was carried out to examine the expression of oxygen-regulated protein 150 (ORP150) using 58 human skin wounds of different ages (group I, 0-12 h; II, 1-5 days; III, 7-14 days; and IV, 17-21 days). In human wound specimens aged 4-12 h, neutrophils recruited at the wound showed no positive signals for ORP150. With the increase in wound age of > or =7 days, granulation tissue and angiogenesis were observed, with the migration of macrophages and fibroblasts with ORP150-positive reactions. In semi-quantitative analysis, the average of ORP150-positive ratios in group III was highest. In group III, all samples had an ORP150-positive ratio of >40%, and 17 samples showed >50%. In group IV, three out of ten samples showed a positive ratio of 40-45% and the remaining seven cases less than 40%. Collectively, with regard to the practical applicability with forensic safety, these observations suggest that an ORP150-positive ratio of >50% strongly indicates a wound age of 7-14 days.
Department of Forensic Medicine, Wakayama Medical University, 811-1 Kimiidera, Wakayama, 641-8509, Japan.
Y
Y
Ishida
A
A
Kimura
T
T
Takayasu
W
W
Eisenmenger
T
T
Kondo
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D002465:Cell Movement; D002648:Child; D005347:Fibroblasts / Q000378:metabolism; D049429:Forensic Pathology; D006097:Granulation Tissue / Q000473:pathology; D018840:HSP70 Heat-Shock Proteins; D006801:Humans; D007150:Immunohistochemistry; D008264:Macrophages / Q000378:metabolism; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D009504:Neutrophils / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D012867:Skin / Q000293:injuries* / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors; D014945:Wound Healing; D055815:Young Adult
D015415:Biomarkers; D018840:HSP70 Heat-Shock Proteins; D011506:Proteins; C050087:oxygen-regulated proteins