Cyclosporin a Up-regulates B7-DC expression on dendritic cells in an IL-4-dependent manner in vitro, which is associated with decreased allostimulatory capacity of dendritic cells.
In addition to the effects on T lymphocytes, Cyclosporin A can inhibit dendritic cells allostimulatory capacity, but its precise mechanism remains unknown. The data in this study demonstrated that Cyclosporin A has no effect on the expression of major histocompatibility complex class II and costimulatory molecular CD80, CD86, CD40 on matured DC induced by Lipopolysaccharide in vitro, but can up-regulated B7-DC expression on DC in an Interleukin-4-dependent manner, which is associated with reduced allostimulatory ability of DC. Furthermore, Cyclosporin A treatment inhibited production of Interferon-gammaand TNF-alpha, Interleukin-12p70, but increased production of the Interleukin-10 of DC. Thus, up-regulated expression of B7-DC may be responsible for Cyclosporin A-mediated inhibitory effects on allostimulatory capacity of DC.
Key Lab of Combined Multi-Organ Transplantation, Ministry of Public Health, Department of Hepato-Biliary-Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Lei
L
Geng
Shuai
S
Dong
Yun
Y
Fang
Guoping
G
Jiang
Haiyang
H
Xie
Miaoda
M
Shen
Sheng
S
Yan
Shusen
S
Zheng
D000818:Animals; D015703:Antigens, CD / Q000096:biosynthesis / Q000276:immunology; D018122:B7-1 Antigen / Q000096:biosynthesis / Q000276:immunology; D016572:Cyclosporine / Q000494:pharmacology*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000096:biosynthesis / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D015847:Interleukin-4 / Q000096:biosynthesis / Q000276:immunology*; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008807:Mice, Inbred BALB C; D015854:Up-Regulation / Q000187:drug effects
D015703:Antigens, CD; D018122:B7-1 Antigen; D016207:Cytokines; D000949:Histocompatibility Antigens Class II; D007166:Immunosuppressive Agents; D008070:Lipopolysaccharides; D015847:Interleukin-4; D016572:Cyclosporine