A novel phytase from Yersinia rohdei with high phytate hydrolysis activity under low pH and strong pepsin conditions.
Two novel phytase genes belonging to the histidine acid phosphatase family were cloned from Yersinia rohdei and Y. pestis and expressed in Pichia pastoris. Both the recombinant phytases had high activity at pH 1.5-6.0 (optimum pH 4.5) with an optimum temperature of 55 degrees C. Compared with the major commercial phytases from Aspergillus niger, Escherichia coli, and a potential commercial phytase from Y. intermedia, the Y. rohdei phytase was more resistant to pepsin, retained more activity under gastric conditions, and released more inorganic phosphorus (two to ten times) from soybean meal under simulated gastric conditions. These superior properties suggest that the Y. rohdei phytase is an attractive additive to animal feed. Our study indicated that, in order to better hydrolyze the phytate and release more inorganic phosphorus in the gastric passage, phytase should have high activity and stability, simultaneously, at low pH and high protease concentration.
Department of Microbial Engineering, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Huoqing
H
Huang
Huiying
H
Luo
Yaru
Y
Wang
Dawei
D
Fu
Na
N
Shao
Guozeng
G
Wang
Peilong
P
Yang
Bin
B
Yao
D010832:6-Phytase / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D004795:Enzyme Stability; D005744:Gastric Acid / Q000737:chemistry; D015870:Gene Expression; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D010434:Pepsin A / Q000737:chemistry; D010833:Phytic Acid / Q000378:metabolism*; D010843:Pichia / Q000235:genetics / Q000378:metabolism; D013025:Glycine max / Q000378:metabolism; D013696:Temperature; D015007:Yersinia / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics
D001426:Bacterial Proteins; D010833:Phytic Acid; D010832:6-Phytase; D010434:Pepsin A