Investigation of the effect of different extracellular factors on the lipase production by Yarrowia lipolityca on the basis of a scale-down approach.
The influence of three extracellular factors (namely, the methyl oleate dispersion in the broth, the dissolved oxygen variations, and the pH fluctuation) on the lipase production by Y. lipolytica in batch bioreactor has been investigated in different scale-down apparatus. These systems allow to reproduce the hydrodynamic phenomena encountered in large-scale equipments for the three specified factors. The effects of the extracellular factors have been observed at three distinct levels: the microbial growth, the extracellular lipase production, and the induction of the gene LIP2 encoding for the main lipase of Y. lipolytica. Among the set of environmental factors investigated, the dissolved oxygen fluctuations generated in a controlled scale-down reactor (C-SDR) have led to the more pronounced physiological effect by decreasing the LIP2 gene expression level. The other environmental factors observed in a partitioned scale-down reactor, i.e., the methyl oleate dispersion and the pH fluctuations, have led to a less severe stress traduced only by a decrease of the microbial yield and thus of the extracellular lipase specific production rate.
Centre Wallon de Biologie Industrielle, Unité de Bio-industries, Faculté Universitaire des Sciences Agronomiques de Gembloux, Passage des déportés, 2, 5030, Gembloux, Belgium.
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Delvigne
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Masson
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Destain
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Thonart
D019149:Bioreactors; D005656:Fungal Proteins / Q000235:genetics; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000096:biosynthesis* / Q000235:genetics; D009829:Oleic Acids / Q000494:pharmacology; D010100:Oxygen / Q000494:pharmacology; D013997:Time Factors; D025062:Yarrowia / Q000166:cytology / Q000187:drug effects / Q000201:enzymology* / Q000254:growth & development
D005656:Fungal Proteins; D009829:Oleic Acids; C005576:methyl oleate; C408971:LIP2 protein, Yarrowia lipolytica; D008049:Lipase; D010100:Oxygen