B cell chemoattractant CXCL13 is preferentially expressed by human Th17 cell clones.
Th 17 cells represent a novel subset of CD4+ T cells that have a protective effect against extracellular microbes, while they are also responsible for autoimmune disorders in mice. However, the protein expression profile of Th17 cells remains to be clarified. In this study, we report an effective method to establish human allo-reactive Th17 cell clones and demonstrate that human Th17, but not Th1 or Th2, cells express B cell chemoattractant CXCL13, by using DNA chips, RT-PCR, and ELISA. Such a pattern was also the case in Candida albicans-specific Th17 clones and synovial fluid specimens obtained from patients with rheumatoid arthritis. The biological implication of this finding is discussed.
Takagi
Rie
R
Department of Allergy and Immunology, Faculty of Medicine, Saitama Medical University Saitama, Japan.
Higashi
Takehiro
T
Hashimoto
Kumiko
K
Nakano
Kazuhisa
K
Mizuno
Yosuke
Y
Okazaki
Yasushi
Y
Matsushita
Sho
S
D000494:Allosteric Regulation / Q000276:immunology; D002478:Cells, Cultured; D054382:Chemokine CXCL13 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D018920:Coculture Techniques; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012333:RNA, Messenger / Q000235:genetics; D013582:Synovial Fluid / Q000276:immunology / Q000378:metabolism; D006377:T-Lymphocytes, Helper-Inducer / Q000276:immunology*; D014158:Transcription, Genetic / Q000235:genetics
C110663:CXCL13 protein, human; D054382:Chemokine CXCL13; D012333:RNA, Messenger