Factor analysis reveals differences in brain metabolism in macaques with SIV/AIDS and those with SIV-induced encephalitis.
MRS has often been used to study metabolic processes in the HIV-infected brain. However, it remains unclear how changes in individual metabolites are related to one another in this context of virus-induced central nervous system dysfunction. We used factor analysis (FA) to identify patterns of metabolite distributions from an MRS study of healthy macaques and those infected with simian immunodeficiency virus (SIV) which were moribund with AIDS. FA summarized the correlations from nine metabolites into three main factors. Factor 3 identified patterns that discern healthy animals from those with SIV/AIDS. Factor 2 was able to differentiate between animals that had encephalitis and those moribund with AIDS but lacking encephalitis. Specifically, Factor 2 was able to distinguish animals with moderate to severe encephalitis from animals with mild or no encephalitis as well as uninfected controls. FA not only confirmed the involvement of neuronal metabolites (N-acetylaspartate and glutamate) in disease severity, but also detected changes in creatine and myo-inositol that have not been observed in the SIV macaque model previously. These results suggest that the divergent pathways of N-acetylaspartate and creatine in this disease may enable the commonly reported ratio N-acetylaspartate/creatine to be a more sensitive marker of disease severity.
Department of Neuroradiology/A. A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA 02129, USA.
Margaret R
MR
Lentz
Vallent
V
Lee
Susan V
SV
Westmoreland
Eva-Maria
EM
Ratai
Elkan F
EF
Halpern
R Gilberto
RG
González
D000818:Animals; D001224:Aspartic Acid / Q000031:analogs & derivatives* / Q000378:metabolism; D001921:Brain / Q000378:metabolism*; D003401:Creatine / Q000378:metabolism*; D004660:Encephalitis / Q000378:metabolism* / Q000662:veterinary*; D005163:Factor Analysis, Statistical; D008253:Macaca mulatta; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D016097:Simian Acquired Immunodeficiency Syndrome / Q000378:metabolism*
D001224:Aspartic Acid; C000179:N-acetylaspartate; D003401:Creatine