Large scale isolation and purification of soluble RAGE from lung tissue.
The receptor for advanced glycation end-products (RAGE) has been implicated in numerous disease processes including: atherosclerosis, diabetic nephropathy, impaired wound healing and neuropathy to name a few. Treatment of animals with a soluble isoform of the receptor (sRAGE) has been shown to prevent and even reverse many disease processes. Isolating large quantities of pure sRAGE for in vitro and in vivo studies has hindered its development as a therapeutic strategy in other RAGE mediated diseases that require long-term therapy. This article provides an improvement in both yield and detail of a previously published method to obtain 10mg of pure, endotoxin free sRAGE from 65 g of lung tissue.
Department of Pathology, University of Pittsburgh School of Medicine, 200 Lothrop Street, W957 BST, Pittsburgh, PA 15261, USA.
Judson M
JM
Englert
Lasse
L
Ramsgaard
Zuzana
Z
Valnickova
Jan J
JJ
Enghild
Tim D
TD
Oury
D000818:Animals; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D008168:Lung / Q000737:chemistry*; D000067759:Receptor for Advanced Glycation End Products / Q000302:isolation & purification*; D012995:Solubility; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry
D000067759:Receptor for Advanced Glycation End Products