[Expression of periostin and the effect of hydrocortisone on it in human fibroblasts of scar].
OBJECTIVE
To probe into the role of periostin in the formation of scars and investigate its reaction to hydrocortisone.


METHODS
Primary fibroblasts were cultured in vitro, then RT-PCR and immunocytochemical technique were used to examine the expressions of mRNA and preotein of periostin respectively in 24 samples of keloid fibroblasts (KFb), hypertrophic scar fibroblasts (HFb) and normal skin fibroblasts (SFb).


RESULTS
The mRNA levels of periostin in KFb (1.645+/-0.549) and HFb (1.084+/-0.396) were both higher than that in SFb (0.274+/-0.215, P<0.05). After hydrocortisone was added, the mRNA levels of periostin in KFb and HFb were both decreased by 32% and 47% respectively (P<0.05 ); The protein of periostin distributed mainly in the endochylema around the nucleus of fibroblasts. The protein levels of periostin in KFb (91.815+/-0.961) and HFb (70.166+/-2.250) were both higher than that in SFb (41.011 +/- 1.576, P<0.01).


CONCLUSIONS
The expressions of periostin were increased in the fibroblasts of hyperplasic scars, which could be inhibited by hydrocortisone. periostin may affect the function of fibroblasts of hyperplasic scars. periostin may be a cicatrix specific gene.
Song
Zhen Hua
ZH
Department of Plastic Surgery, Peking University Third Hospital, Beijing, China.
Qin
Ze Lian
ZL
D002056:Burns / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D017439:Cicatrix, Hypertrophic / Q000209:etiology / Q000378:metabolism* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D006854:Hydrocortisone / Q000494:pharmacology*; D007627:Keloid / Q000378:metabolism* / Q000473:pathology; D008297:Male; D014945:Wound Healing
D015815:Cell Adhesion Molecules; C512835:POSTN protein, human; D006854:Hydrocortisone