Co-expression and preferential interaction between two calcineurin B-like proteins and a CBL-interacting protein kinase from cotton.
The CBL/CIPK signaling system mediates a variety of responses to environmental stimuli in plants. In this work, we identified four CBL genes from Gossypium hirsutum, two of which (designated GhCBL2 and GhCBL3) showed preferential expression in the elongating fiber cells. Moreover, the expression patterns of these two CBL genes coincided with that of a putative CBL-interacting protein kinase gene (GhCIPK1) that we isolated in a previous study. Yeast two-hybrid assay indicated that among the four CBLs, GhCIPK1 interacted selectively with GhCBL2 and GhCBL3. The co-expression and interactions of these proteins suggest that they are components of the same signaling pathway. These findings strengthen our previous prediction that CBL/CIPK signaling plays a critical role in the regulation of cotton fiber elongation.
National Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Datun Road, Chaoyang District, Beijing, China.
Peng
P
Gao
Pi-Ming
PM
Zhao
Juan
J
Wang
Hai-Yun
HY
Wang
Xiong-Ming
XM
Du
Gui-Ling
GL
Wang
Gui-Xian
GX
Xia
D000595:Amino Acid Sequence; D001483:Base Sequence; D019703:Calcineurin / Q000737:chemistry / Q000378:metabolism*; D017931:DNA Primers; D003368:Gossypium / Q000201:enzymology / Q000378:metabolism*; D008969:Molecular Sequence Data; D010940:Plant Proteins / Q000737:chemistry / Q000378:metabolism*; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D010940:Plant Proteins; D019703:Calcineurin