Resonance light-scattering enhancement effect of the protein-Y3+-TTA-SLS system and its analytical application.
In this paper, a sensitive resonance light scattering (RLS) method for the determination of protein is reported. In the Tris-HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y(3+)-2-thenoyltrifluoroacetone (TTA)-sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 x 10(-9)-1.0 x 10(-5) g/mL for BSA, 1.0 x 10(-8)-1.0 x 10(-5) g/mL for HSA and 1.0 x 10(-8)-1.0 x 10(-6 )g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied.
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, People's Republic of China.
Changying
C
Guo
Xia
X
Wu
Wei
W
Xu
Jinghe
J
Yang
D002021:Buffers; D002614:Chelating Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D013804:Thenoyltrifluoroacetone / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*
D002021:Buffers; D002614:Chelating Agents; D011506:Proteins; D012709:Serum Albumin; D013501:Surface-Active Agents; D013804:Thenoyltrifluoroacetone; D012967:Sodium Dodecyl Sulfate; D015019:Yttrium; D010047:Ovalbumin; C091417:yttria