[Electrophysiological characteristics of ion transport in isolated rabbit trachea wall after in effect of mechanical stimuli and sodium transport inhibition].
BACKGROUND
The aim of the study was to determine a role of intra-wall nervous and neurohormonal system in control of ion transport in airways, and to identify the control mechanisms, having effect on constant electric potential of this tissue named as PD, and the ones which have effect on reversible changes of this potential marked as dPD. Through the application of amiloride sodium ion transport blocker the importance of transepithelial sodium ion transport for transepithelial electric potential of airways was to be determined, as well as the other amiloride effects on isolated airways. The conditions for examination of secretion of the chlorides in airways have been determined and will be presented as chemical isolation of chloride currents with use of amiloride.


METHODS
Experimental material consisted of 135 fragments of trachea wall obtained from 45 animals. Experiments consisted in measurements of transepithelial electric potential (PD) and resistance (R) of isolated trachea wall placed in Ussing apparatus, where the tissue separates two chambers filled up with isoosmotic complex electrolyte solution. The essential procedure applied in irritation of sensory receptors was directing the fluid jet from a peristaltic pump to mucous surface of isolated trachea. The jet consisted of fluid analogous to the one from the chamber, or it was modified as the experiment conditions required.


RESULTS
Transepithelial transport way of sodium ions in trachea epithelium has the controlling effect in value modulation of transepithelial difference of electric potentials, as well as in induction of hyperpolarization after mechanical stimuli action, while at the same time the sodium transport at 40% is the exclusive carrier of hyperpolarization reaction.
Katedra i Zakład Patofizjologii Pomorskiej Akademii Medycznej w Szczecinie, Szczecin.
Bolesław
B
Banach
D000584:Amiloride / Q000494:pharmacology; D000818:Animals; D066298:In Vitro Techniques; D017136:Ion Transport / Q000502:physiology*; D008564:Membrane Potentials; D010812:Physical Stimulation; D011817:Rabbits; D012964:Sodium / Q000378:metabolism*; D026941:Sodium Channel Blockers / Q000494:pharmacology; D015222:Sodium Channels / Q000378:metabolism*; D014132:Trachea / Q000378:metabolism*
D026941:Sodium Channel Blockers; D015222:Sodium Channels; D000584:Amiloride; D012964:Sodium