[Proteomic analysis of Helicobacter pylori in human gastritis and gastric cancer].
OBJECTIVE
To establish 2-dimensional electrophoresis (2-DE) maps of Helicobac-ter pylori in human gastritis, and gastric cancer, to identify the differentially expressed proteins,and to discuss the role of bacterial factor in pathogenesis.


METHODS
The total proteins of Helicobacter pylori in human gastritis and gastric cancer were separated by immobilized pH gradient-based 2-DE. The differentially expressed proteins were screened by PDQuest analysis software and identified by peptide mass fingerprint based on matrix-assisted laser desorption/ionization time of flight mass spectrometry, and searched on database.


RESULTS
A well-resolved and reproducible 2-DE pattern of Helicobacter pylori was obtained from patients with human gastritis and gastric cancer. Fourteen differentially expressed proteins were identified, including proteins related to anti-oxidation,molecular chape-rones and detoxification, enzymes related to metabolism,proteins related to cytoarchitecture,and proteins related to signal conduction.


CONCLUSIONS
A well-resolved and reproducible 2-DE pattern of Helicobacter pylori in human gastritis and gastric cancer is established and differentially expressed proteins from these 2 diseases are identified. The differentiation of protein expression may play an important role in the pathogenesis of gastric cancer.
Department of Rheumatology and Immunology, Xiangya Hospital, Central South University, Changsha, China.
Yun-hui
YH
You
Xue-gong
XG
Fang
Ping
P
Liu
Hong-bo
HB
Liu
Xue-fei
XF
Tian
Xue-mei
XM
Yan
D001426:Bacterial Proteins / Q000032:analysis*; D015180:Electrophoresis, Gel, Two-Dimensional; D005260:Female; D005756:Gastritis / Q000382:microbiology; D016481:Helicobacter Infections / Q000382:microbiology*; D016480:Helicobacter pylori; D006801:Humans; D008297:Male; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D013274:Stomach Neoplasms / Q000382:microbiology*
D001426:Bacterial Proteins; D020543:Proteome