Usefulness of Sau-PCR for molecular epidemiology of nosocomial outbreaks due to Burkholderia cepacia which occurred in a local hospital in Guangzhou, China.
The aim of the present study was to determine the source of nosocomial outbreak due to Burkholderia cepacia by molecular techniques. A total of 11 B. cepacia strains were isolated; nine from blood and one from sputum of patients without cystic fibrosis, and one from reverse osmosis water at a local hospital in Guangzhou, China. Analyses of 11 strains by the Sau-PCR assay and pulsed-field gel electrophoresis revealed that nine strains obtained from the blood of outpatients in a hemodialysis unit and one strain from reverse osmosis water had identical DNA profiles, indicating that the reverse osmosis water supply could be a source of infection.
Yan
He
H
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China.
Shi
Lei
L
Alam
M J
MJ
Li
Lin
L
Yang
Liansheng
L
Yamasaki
Shinji
S
D000328:Adult; D000368:Aged; D015373:Bacterial Typing Techniques / Q000379:methods*; D001769:Blood / Q000382:microbiology; D019121:Burkholderia Infections / Q000382:microbiology*; D016956:Burkholderia cepacia / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002681:China; D003428:Cross Infection / Q000382:microbiology*; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000235:genetics*; D004196:Disease Outbreaks; D016521:Electrophoresis, Gel, Pulsed-Field; D006761:Hospitals; D006801:Humans; D008875:Middle Aged; D017720:Molecular Epidemiology / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*; D013183:Sputum / Q000382:microbiology; D014871:Water Microbiology
D004269:DNA, Bacterial