Dipicolinic acid-based disk methods for detection of metallo-beta-lactamase-producing Pseudomonas spp. and Acinetobacter spp.
We evaluated novel and simple dipicolinic acid (DPA)-based phenotypic screening methods to detect metallo-beta-lactamase (MBL)-producing isolates: DPA disk synergy tests and DPA-based disk tests (DPA-imipenem, DPA-meropenem, or DPA-ceftazidime disk tests). Upon testing these methods on 79 MBL-producing and 95 MBL-nonproducing isolates of Pseudomonas spp. and Acinetobacter spp., their specificity and sensitivity were superior or comparable with those of the EDTA-based phenotypic screening methods.
Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, Chungbuk 361-711, South Korea. ksshin@chungbuk.ac.kr
Kyeong Seob
KS
Shin
Bo Ra
BR
Son
Seung Bok
SB
Hong
Jungmin
J
Kim
D000150:Acinetobacter / Q000145:classification / Q000187:drug effects* / Q000201:enzymology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D052978:Disk Diffusion Antimicrobial Tests / Q000379:methods*; D004492:Edetic Acid; D006785:Hospitals, University; D006801:Humans; D007723:Korea; D010848:Picolinic Acids / Q000494:pharmacology*; D011237:Predictive Value of Tests; D011549:Pseudomonas / Q000145:classification / Q000187:drug effects* / Q000201:enzymology; D012680:Sensitivity and Specificity; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D010848:Picolinic Acids; D047090:beta-Lactams; D004492:Edetic Acid; D001618:beta-Lactamases; C004860:dipicolinic acid