Simultaneous separation by reversed-phase high-performance liquid chromatography and mass spectral identification of anthocyanins and flavonols in Shiraz grape skin.
A limitation of large-scale viticultural trials is the time and cost of comprehensive compositional analysis of the fruit by high-performance liquid chromatography (HPLC). In addition, separate methods have generally been required to identify and quantify different classes of metabolites. To address these shortcomings a reversed-phase HPLC method was developed to simultaneously separate the anthocyanins and flavonols present in grape skins. The method employs a methanol and water gradient acidified with 10% formic acid with a run-time of 48 min including re-equilibration. Identity of anthocyanins and flavonols in Shiraz (Vitis vinifera L.) skin was confirmed by mass spectral analysis.
Downey
Mark O
MO
Viticulture, Future Farming Systems Research, Department of Primary Industries Victoria, P.O. Box 905, Mildura, Vic. 3502, Australia. mark.downey@dpi.vic.gov.au
Rochfort
Simone
S
D000434:Alcoholic Beverages / Q000032:analysis; D000872:Anthocyanins / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D044948:Flavonols / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D027843:Vitis / Q000737:chemistry*
D000872:Anthocyanins; D044948:Flavonols