Polymorphic microsatellite loci for the common marmoset (Callithrix jacchus) designed using a cost- and time-efficient method.
We describe a cost- and time-efficient method for designing new microsatellite markers in any species with substantial genomic DNA sequence data available. Using this technique, we report 14 new polymorphic dinucleotide microsatellite loci isolated from the common marmoset. The relative yield of new polymorphisms was higher with less labor than described in previous marmoset studies. Of 20 loci initially evaluated, 14 were polymorphic and amplified reliably (70% success rate). The number of alleles ranged from 3 to 9 with heterozygosity varying from 0.48 to 0.83.
Raveendran
M
M
Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78227, USA. mraveend@sfbrgenetics.org
Tardif
S
S
Ross
C N
CN
Austad
S N
SN
Harris
R A
RA
Milosavljevic
A
A
Rogers
J
J
D000818:Animals; D001483:Base Sequence; D002144:Callithrix / Q000235:genetics*; D004247:DNA / Q000235:genetics; D016678:Genome; D006801:Humans; D018895:Microsatellite Repeats; D016133:Polymerase Chain Reaction / Q000191:economics / Q000379:methods; D011110:Polymorphism, Genetic; D013997:Time Factors
D004247:DNA