Effect of protein degradation on spot M(r) distribution in 2-D gels--a case study of proteolysis during development of Streptomyces coelicolor cultures.
Proteolysis, a regulated biological process, is reflected by protein spot molecular weight distribution in 2-D gel electrophoretograms. Here we report studies of Streptomyces cultures as they undergo two different developmental processes involving proteolysis. Systematic changes in protein molecular weight distribution between the control samples and those with high activity of proteases were demonstrated. The observations were supported by a numerical model of degradation and its influence on the M(r) distribution. Simple statistics could be used to distinguish between normal and degradative 2-D gel electrophoretic patterns.
Vohradsky
Jiri
J
Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic. vohr@biomed.cas.cz
Branny
Pavel
P
Li
Xin-Ming
XM
Thompson
Charles J
CJ
D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D002138:Calibration; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D005782:Gels; D006868:Hydrolysis; D008970:Molecular Weight; D010449:Peptide Mapping; D012015:Reference Standards; D048372:Streptomyces coelicolor / Q000254:growth & development*; D013997:Time Factors
D001426:Bacterial Proteins; D003470:Culture Media; D005782:Gels