Methanol extract of Ficus leaf inhibits the production of nitric oxide and proinflammatory cytokines in LPS-stimulated microglia via the MAPK pathway.
Excessive production of inflammatory mediators, nitric oxide (NO) and proinflammatory cytokines from activated microglia has been implicated in neurodegeneration in human brain diseases. Recently, it seems possible that treatment with antiinflammatory agents, including Oriental medicinal plants, might delay the progression of neurodegeneration through the inhibition of microglial activation. The present study evaluated the effect of a methanol extract of Ficus religiosa leaf (MFL) on lipopolysaccharide (LPS)-induced production of NO and proinflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interleukin-beta (IL-1beta) and IL-6 in BV-2 cells, a mouse microglial line. MFL inhibited LPS-induced production of NO and proinflammatory cytokines in a dose-dependent manner. MFL also attenuated the expression of mRNA and proteins of inducible nitric oxide synthase (iNOS) and proinflammatory cytokines, suggesting the blockage of transcription levels, respectively. The molecular mechanism of MFL-mediated attenuation underlies the down-regulation of the extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) signaling pathway, and suppresses the nuclear factor kappaB (NF-kappaB) activation. The results suggest that MFL exhibits antiinflammatory properties in LPS-induced activation of BV2 microglial cells, and that might have a therapeutic potential for various neurodegenerative diseases.
Department of Herbology, College of Oriental Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea.
Hyo Won
HW
Jung
Hye Young
HY
Son
Chau Van
CV
Minh
Young Ho
YH
Kim
Yong-Ki
YK
Park
D000818:Animals; D002460:Cell Line; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D030681:Ficus / Q000737:chemistry*; D015870:Gene Expression / Q000187:drug effects; D008070:Lipopolysaccharides / Q000494:pharmacology; D000432:Methanol / Q000737:chemistry; D051379:Mice; D017628:Microglia / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D020929:Mitogen-Activated Protein Kinase Kinases / Q000502:physiology; D009569:Nitric Oxide / Q000378:metabolism*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism
D016207:Cytokines; D008070:Lipopolysaccharides; D010936:Plant Extracts; D012333:RNA, Messenger; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D020929:Mitogen-Activated Protein Kinase Kinases; D000432:Methanol