Serologically defined Rhipicephalus (Boophilus) microplus larval antigens in BmLF3, a partially pure Sephacryl S-300 fraction of crude larval proteins.
This report is designed to provide additional information regarding larval soluble proteins toward the planned development of a comprehensive database of Rhipicephalus (Boophilus) microplus proteins that elicit a humoral immune response in cattle as a result of natural ectoparasite infestation. Larval proteins of R. microplus are complex and the protein profile is not dominated by any major proteins. This report focuses upon an S-300 Sephacryl (molecular sieve) column fraction, fraction 3 (BmLF3). With the use of SDS-PAGE (without-2ME) and Western blotting with a composite pool of pre- and post-R. microplus larval infestation antiserum BmLF3 was found to contain 7 apparent common ixodid major antigens (207.3, 171.9, 98.0, 86.5, 65.7, 58.9, and 38.0 kDa), those potentially shared with other ixodid species, and 2 apparent R. microplus specific antigens evidenced by low-level antibody binding in crude BmLF3 (149.4 kDa) and HPLC peak 8 of BmLF3 (116.0 kDa). In addition, BmLF3 contains potent inhibitors of trypsin activity. However, these inhibitors of trypsin did not appear to elicit host antibodies as a result of natural ectoparasite exposure, as defined by Western blotting of reduced and denatured trypsin binding proteins purified by affinity chromatography.
Knipling-Bushland, U.S. Livestock Insects Research Laboratory, 2700 Fredericksburg Road, Kerrville, TX 78028, USA. john.pruett@ars.usda.gov
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Olafson
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Davey
D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D000941:Antigens / Q000276:immunology* / Q000302:isolation & purification; D015153:Blotting, Western; D002417:Cattle; D002418:Cattle Diseases / Q000276:immunology* / Q000469:parasitology; D005591:Chemical Fractionation; D002847:Chromatography, Agarose; D002851:Chromatography, High Pressure Liquid; D004791:Enzyme Inhibitors / Q000302:isolation & purification; D007106:Immune Sera / Q000276:immunology; D007814:Larva / Q000737:chemistry / Q000276:immunology; D048494:Rhipicephalus / Q000737:chemistry* / Q000276:immunology*; D013984:Tick Infestations / Q000276:immunology / Q000662:veterinary*; D014357:Trypsin / Q000378:metabolism
D000180:Acrylic Resins; D000941:Antigens; D004791:Enzyme Inhibitors; D007106:Immune Sera; C037239:Sephacryl Superfine; D014357:Trypsin