[Screening for differentially expressed proteins in nasopharyngeal carcinoma by laser capture microdissection and proteomic analysis].
OBJECTIVE
To search for the differentially expressed proteins of nasopharyngeal carcinoma (NPC),and provide scientific evidence for identifying molecular biomarkers for NPC.


METHODS
Laser capture microdissection (LCM) was used to purify the target cells from NPC and normal nasopharyngeal epithelial tissues (NNET). Two-dimensional gel electrophoresis (2-DE) was used to separate the total proteins of microdissected NPC and NNET, PDQuest software was applied to analyze 2-DE images,and the differential proteins between the 2 types of tissues were identified by both MALDI-TOF-MS and ESI-Q-TOF-MS. Western blot and immunohistochemistry of tissue microarray were used to detect the expression of the differential protein SCCA1 in NPC and NNET.


RESULTS
2-DE patterns of microdissected NPC and NNEC were established,and 36 differential proteins in the NPC and NNEC were identified,20 of which only expressed or up-regulated in NPC and 16 only expressed or up-regulated in NNET. The differentially expressed level of SCCA1 in the NPC and NNET was confirmed by Western blot and immunohistochemistry of tissue microarray.


CONCLUSIONS
Thirty-six differentially expressed proteins identified in this study may be associated with the carcinogenesis of NPC,and may be candidate molecular biomarkers for NPC.
Yi
Hong
H
Medical Research Center, Xiangya Hospital, Central South University, Changsha, China.
Cheng
Ai-lan
AL
Huang
Wei-guo
WG
Zhang
Peng-fei
PF
Li
Mao-yu
MY
Peng
Fang
F
Li
Feng
F
Li
Cui
C
Chen
Zhu-chu
ZC
Xiao
Zhi-qiang
ZQ
D000595:Amino Acid Sequence; D000951:Antigens, Neoplasm / Q000302:isolation & purification*; D014408:Biomarkers, Tumor / Q000302:isolation & purification; D002294:Carcinoma, Squamous Cell / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D007834:Lasers; D042282:Microdissection / Q000379:methods*; D008969:Molecular Sequence Data; D009303:Nasopharyngeal Neoplasms / Q000737:chemistry*; D009363:Neoplasm Proteins / Q000302:isolation & purification*; D040901:Proteomics / Q000379:methods*; D015843:Serpins / Q000302:isolation & purification*
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D015843:Serpins; C051072:squamous cell carcinoma-related antigen