Potential inhibitory effects of D-allose, a rare sugar, on liver preneoplastic lesion development in F344 rat medium-term bioassay.
D-allose, the C-3 epimer of d-glucose, is a monosaccharide present in minute quantities in nature and a rare sugar. The effects of D-allose on diethyl nitrosamine (DEN)-induced hepatocarcinogenesis were examined in male F344 rats by a rat medium-term bioassay based on the two-step model of hepatocarcinogenesis (experiment 1). In addition, a DNA microarray analysis was employed to clarify possible mechanisms of action of D-allose (experiment 2). The antioxidation potential of D-allose solution itself or of serum in rats treated with D-allose was also examined directly by measuring Cu(+)-reducing antioxidation power (experiment 3). Furthermore, to investigate the effects of D-allose in vivo under conditions of oxidative stress, it was administered with a choline-deficient, L-amino acid-defined diet (CDAA) in the medium-term liver carcinogenesis bioassay (experiment 4). Experiment 1 demonstrated no effects of D-allose on the development of glutathione S-transferase placental form (GST-P) positive foci in the liver. From DNA microarray analysis, several mRNA markers were found to be altered with functions related to apoptosis and cell proliferation (experiment 2), although D-allose itself and serum in vivo exhibited no antioxidation power directly (experiment 3). When D-allose was administered with the CDAA diet, decreases in the area and number of GST-P positive foci were noted with P values of 0.158 for area (%) and 0.061 for number (/cm(2)) (experiment 4). These results suggest the potential inhibitory effect of D-allose on liver carcinogenesis, particularly under oxidative stress conditions.
Department of Pathology and Host-Defence, Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Kagawa, Japan.
Masanao
M
Yokohira
Kyoko
K
Hosokawa
Keiko
K
Yamakawa
Kousuke
K
Saoo
Yoko
Y
Matsuda
Yu
Y
Zeng
Toshiya
T
Kuno
Katsumi
K
Imaida
D000818:Animals; D016207:Cytokines / Q000276:immunology*; D005947:Glucose / Q000008:administration & dosage*; D008113:Liver Neoplasms / Q000276:immunology* / Q000517:prevention & control*; D008297:Male; D011230:Precancerous Conditions / Q000276:immunology* / Q000517:prevention & control*; D051381:Rats; D011916:Rats, Inbred F344; D016896:Treatment Outcome
D016207:Cytokines; C002055:allose; D005947:Glucose