Quantitative analysis of the neurons from the myenteric plexus in the ileum of rats submitted to severe protein deficiency.
The effects of protein malnutrition on the quantitative aspects of the myenteric plexus in the ileum of adult Rattus norvegicus were assessed. Thirty 90-day-old rats were divided into two groups: Control Group (CG, n=15) and Experimental Group (EG, n=15). The CG received 26% protein chow and the EG received 4% protein chow for 90 days. At the end of the experiment, the animals from the CG weighed 369.63+/-26.33, and the ones from the EG 215.34+/-56.31. The ileum was submitted to Giemsa, NADH- and NADPH-diaphorase technique in order to evidence nervous cells in the whole-mount preparations. Animals from the EG presented a 41.75% body weight loss in relation to the CG as well as 17.6% length reduction for the ileum-jejunum. Moreover, the organ was 41% lighter for the EG. Giemsa-stained neurons were 17.02% more concentrated in the EG (p>0.05). NADH-diaphorase-stained neurons were 26.6% more concentrated in the EG (p<0.05), while the NADPH-diaphorase were 26.28% more concentrated in this group (p<0.05).
Moreira
Neide Martins
NM
Experimental Neurogastroenterology Laboratory, UNIPAR, Umuarama, PR, Brazil.
Hermes
Catchia
C
Almeida
Carla Simone Leite de
CS
Santana
Evelyne Cruz
EC
Sant'ana
Débora de Mello Gonçales
Dde M
Araújo
Eduardo José de Almeida
EJ
D000818:Animals; D001835:Body Weight; D002452:Cell Count; D007082:Ileum / Q000201:enzymology / Q000294:innervation*; D008297:Male; D009197:Myenteric Plexus / Q000166:cytology* / Q000201:enzymology; D009252:NADPH Dehydrogenase / Q000378:metabolism*; D009474:Neurons / Q000166:cytology* / Q000201:enzymology; D009929:Organ Size; D011488:Protein Deficiency / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar
D009252:NADPH Dehydrogenase