Initial stage of development and migratory behavior of Toxocara canis larvae in BALB/c mouse experimental model.
In the present study, the initial developmental stage of Toxocara canis eggs and larvae, and number of recovered larvae from BALB/c mouse-infected organs are described. In vitro culture of T. canis detects the frequencies of interphasic, mitotic and embryonated eggs only within a 7-day period. Analysis by egg counting was carried out for 32 days. The results showed that at 7 days after cultivation, the frequency of larvae was 50.4% and that this frequency reached 52.8% in 32 days. In the experimental infection of BALB/c mice with T. canis, the number of recovered larvae statistically increased in the brain and liver, with doses of approximately 200 and 1000 eggs. After 7 days of infection, a larger number of larvae were obtained in the lung and liver, although a maximum amount was found in the brain after a 15- or 30-day post-infection period.
Departamento de Biologia Celular e Genética, Maringá, PR, Brasil.
M L
ML
Camparoto
B
B
Fulan
C M
CM
Colli
M L
ML
Paludo
A L
AL
Falavigna-Guilherme
M A
MA
Fernandez
D000818:Animals; D001921:Brain / Q000469:parasitology; D004195:Disease Models, Animal; D005260:Female; D007814:Larva / Q000254:growth & development; D008099:Liver / Q000469:parasitology; D008168:Lung / Q000469:parasitology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D010270:Parasite Egg Count; D013997:Time Factors; D017166:Toxocara canis / Q000254:growth & development*; D014120:Toxocariasis / Q000469:parasitology*