Channa punctata brain metallothionein is a potent scavenger of superoxide radicals and prevents hydroxyl radical-induced in vitro DNA damage.
Mammalian brain metallothioneins (MTs) have been shown to scavenge free radicals. However, a similar role for fish brain MT has not been established yet. Previously, we have reported that MT from the liver of a freshwater fish, Channa punctata Bloch, had free-radical-scavenging activity in vitro. In this study, we report on the induction of MT in brain and other tissues of C. punctata treated with a low concentration of zinc chloride. We partially purified MT (Zn-MT)-rich fraction from the brain and studied its free-radical-scavenging and DNA damage attenuating effects. Zinc exposure showed significant MT induction in brain, gill, kidney, and liver. C. punctata brain MT efficiently scavenged superoxide radicals and also attenuated hydroxyl radical-mediated DNA damage. These findings suggest that fish brain MT has a free-radical-scavenging activity, and its expression may be regulated in response to stress and chemical exposure. C. punctata has been identified as a potent biomarker fish species. It is suggested that this fish species may be a good model for the study of MTs with regard to their regulatory and biomarker functions.
Atif
Fahim
F
Department of Medical Elementology and Toxicology, Jamia Hamdard (Hamdard University), New Delhi, India.
Kaur
Manpreet
M
Ansari
Rizwan A
RA
Raisuddin
Sheikh
S
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D002712:Chlorides / Q000494:pharmacology; D004247:DNA / Q000737:chemistry; D004249:DNA Damage / Q000187:drug effects*; D004587:Electrophoresis, Agar Gel; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000737:chemistry / Q000633:toxicity; D008668:Metallothionein / Q000096:biosynthesis / Q000302:isolation & purification / Q000494:pharmacology*; D010473:Perciformes / Q000378:metabolism*; D013481:Superoxides / Q000737:chemistry*; D017967:Zinc Compounds / Q000494:pharmacology
D002712:Chlorides; D016166:Free Radical Scavengers; D017967:Zinc Compounds; D013481:Superoxides; D017665:Hydroxyl Radical; C016837:zinc chloride; D004247:DNA; D008668:Metallothionein