Reliability of different image analysis methods for scanning laser Doppler flowmetry.
OBJECTIVE
To assess reliability and reproducibility of different analysis methods for retinal capillary flow, volume, and velocity from scanning laser Doppler flowmetry (SLDF) topography.


METHODS
SLDF topography analysis using the default retinal flowmeter (HRF) were compared to that using automatic full-field perfusion image analyzer (AFFPIA) and quantified as intraclass correlation coefficients (ICC).


RESULTS
The AFFPIA full-field method had the highest reliability, with ICC 0.99 for capillary flow. The reproducibility using the AFFPIA full-field method was high ICC 0.74 for capillary flow.


CONCLUSIONS
The AFFPIA full-field method is highly reliable and superior to the default HRF software.
Kreis
Andreas J
AJ
Centre for Eye Research Australia, University of Melbourne, Australia.
Nguyen
Thanh
T
Rogers
Sophie
S
Wang
Jie Jin
JJ
Harazny
Joanna
J
Michelson
Georg
G
Farouque
H M Omar
HM
Wong
Tien Y
TY
D000328:Adult; D001783:Blood Flow Velocity / Q000502:physiology; D001810:Blood Volume / Q000502:physiology; D002196:Capillaries / Q000502:physiology; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017078:Laser-Doppler Flowmetry / Q000592:standards*; D008297:Male; D008875:Middle Aged; D015588:Observer Variation; D015203:Reproducibility of Results; D012171:Retinal Vessels / Q000502:physiology*