Oxidative stress biomarkers in sporadic ALS.
We aimed to investigate oxidative stress biomarkers in a cross-sectional pilot study of 50 participants with sporadic ALS (SALS) compared to 46 control subjects. We measured urinary 8-oxodeoxyguanosine (8-oxodG), urinary 15-F(2t)-isoprostane (IsoP), and plasma protein carbonyl by ELISA methods. We also determined if ELISA measurement of 8-oxodG could be validated against measures from high-pressure liquid chromatography coupled with electrochemical detection, the current standard method. We found that 8-oxodG and IsoP levels adjusted for creatinine were significantly elevated in SALS participants. These differences persisted after age and gender were controlled in regression analyses. These markers are highly and positively correlated with each other. 8-oxodG measured by the two techniques from the same urine sample were positively correlated (p<.0001). Protein carbonyl was not different between SALS participants and controls. In conclusion, using ELISA, we confirmed that certain oxidative stress biomarkers were elevated in SALS participants. ELISA may be reliable and thus useful in epidemiology studies requiring large numbers of samples to determine the significance of increased oxidative stress markers in SALS. Further studies are required.
Mitsumoto
Hiroshi
H
Eleanor and Lou Gehrig MDA/ALS Research Center, Columbia University Medical Center, New York, NY 10032, USA. hm264@columbia.edu
Santella
Regina M
RM
Liu
Xinhua
X
Bogdanov
Mikhail
M
Zipprich
Jennifer
J
Wu
Hui-Chen
HC
Mahata
Julie
J
Kilty
Mary
M
Bednarz
Kate
K
Bell
Daniel
D
Gordon
Paul H
PH
Hornig
Mady
M
Mehrazin
Mahsa
M
Naini
Ali
A
Flint Beal
M
M
Factor-Litvak
Pam
P
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000453:epidemiology* / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood* / Q000652:urine*; D001798:Blood Proteins / Q000378:metabolism; D003430:Cross-Sectional Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010865:Pilot Projects; D017382:Reactive Oxygen Species
D015415:Biomarkers; D001798:Blood Proteins; D017382:Reactive Oxygen Species; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine