Innovative technique for the study of pneumoperitoneum in the evaluation of abdominal pain after chemical irritation with zymozan.
OBJECTIVE
To assess the influence of pneumoperitoneum in mice submitted to peritoneal irritation provoked by the biological agent Saccharomyces cerevisae, by counting the number of abdominal contractions elicited.


METHODS
To study the effects of pneumoperitoneum analgesic action, 60 mice were divided into two groups: the experimental group, subjected to pneumoperitoneum; and the control group, without pneumoperitoneum. The both groups received intraperitoneal injection of zymosan at a dose of 1mg/0,2ml/mouse.


RESULTS
The sum of the number of abdominal contractions of the experimental group (with pneumoperitoneum) was significantly lower than that of the control group (without pneumoperitoneum). In the experimental group, a lower number of contractions occurred in each min compared to the control.


CONCLUSIONS
The observation of the analgesic effect of pneumoperitoneum using CO2 in mice submitted to peritoneal irritation by zymosan was verified.
Crema
Eduardo
E
Department of Digestive Surgery, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil. cremauftm@mednet.com.br
Silva
Bruna Ferrante
BF
Lamounier e Silva
Pauliana
P
Rodrigues Júnior
Virmondes
V
Cunha
Fernando
F
D015746:Abdominal Pain / Q000209:etiology / Q000503:physiopathology*; D000818:Animals; D004195:Disease Models, Animal; D007274:Injections, Intraperitoneal; D007509:Irritants; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009119:Muscle Contraction / Q000187:drug effects*; D010147:Pain Measurement / Q000295:instrumentation*; D011028:Pneumoperitoneum, Artificial / Q000009:adverse effects; D012441:Saccharomyces cerevisiae; D015054:Zymosan
D007509:Irritants; D015054:Zymosan