[Influence of maggot secretion on expression of bFGF and connective tissue growth factor in ulcer tissue of diabetes mellitus rat and antibacterium study].
OBJECTIVE
To study the influence of maggot secretion on expression of bFGF and connective tissue growth factor (CTGF) in ulcer tissue of diabetes mellitus (DM) rats and its antibacterial function.


METHODS
There were 40 3-month-old SD male rats (weighing 300-350 g) which were randomly divided into 2 groups: control group and experimental maggot secretion group. The model of ulcer wound of DM rats was made. The ulcer wound of DM rats in maggot secretion group spread maggot secretions, but no secretion on ulcer wound was found in control group. The morphological and tissue changes of ulcer wound were observed at different times, and the conditions of bacterial infection on ulcer wound in the two groups were checked. Tissue slices were prepared on 7, 14 and 21 days, respectively; immunohistological detection of bFGF and CTFG in ulcer wound of the two groups was done; and the cell number of positive expression of bFGF and CTFG was counted.


RESULTS
It was found that the healing of ulcer was dominant in experimental group; the wound was clean; the tissue regenerated and no Staphylococcus aureus infection was seen. Bad healing was obtained in control group; tissue necrosis was found and the rate of Staphylococcus aureus infection was 60%. Positive expression cell number of bFGF in ulcer wound was detected on 7 and 14 days after operation with 23.76 +/- 3.34 and 52.76 +/- 4.84 in experimental group, and 18.88 +/- 2.16 and 46.04 +/- 4.00 in control group. Positive expression cell number of CTGF in ulcer wound was detected on 7 and 14 days after operation with 18.76 +/- 3.24 and 46.52 +/- 4.07 in experimental group, and 12.52 +/- 3.03 and 40.52 +/- 3.96 in control group. There was significant difference between positive expressions of bFGF and CTFG in the two groups (P < 0.05).


CONCLUSIONS
The maggot secretion can elevate the expressions of bFGF and CTFG in ulcers, promote healing and prevent bacterial infection.
Department of Orthopedics, First Affiliated Hospital of Dalian Medical University, Dalian Liaoning, 116021, P.R. China.
Shouyu
S
Wang
Decheng
D
Lv
Yuanyuan
Y
Wang
Jiangning
J
Wang
D000818:Animals; D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D001424:Bacterial Infections / Q000517:prevention & control; D055513:Connective Tissue Growth Factor / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007814:Larva / Q000737:chemistry; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012883:Skin Ulcer / Q000378:metabolism* / Q000473:pathology; D014945:Wound Healing / Q000187:drug effects*; D014947:Wounds and Injuries / Q000378:metabolism
D000900:Anti-Bacterial Agents; D016222:Fibroblast Growth Factor 2; D055513:Connective Tissue Growth Factor