Thalamic nuclei in the opossum Monodelphis domestica.
We investigated nuclear divisions of the thalamus in the gray short-tailed opossum (Monodelphis domestica) to gain detailed information for further developmental and comparative studies. Nissl and myelin staining, histochemistry for acetylcholinesterase and immunohistochemistry for calretinin and parvalbumin were performed on parallel series of sections. Many features of the Monodelphis opossum thalamus resemble those in Didelphis and small eutherians showing no particular sensory specializations, particularly in small murid rodents. However, several features of thalamic organization in Monodelphis were distinct from those in rodents. In the opossum the anterior and midline nuclear groups are more clearly separated from adjacent structures than in eutherians. The dorsal lateral geniculate nucleus (LGNd) starts more rostrally and occupies a large part of the lateral wall of the thalamus. As in other marsupials, two cytoarchitectonically different parts, alpha and beta are discernible in the LGNd of the opossum. Each of them may be subdivided into two additional bands in acetylcholinesterase staining, while in murid rodents the LGNd consists of a homogeneous mass of cells. Therefore, differentiation of the LGNd of the Monodelphis opossum is more advanced than in murid rodents. The medial geniculate body consists of three nuclei (medial, dorsal and ventral) that are cytoarchitectonically distinct and stain differentially for parvalbumin. The relatively large size of the MG and LGNd points to specialization of the visual and auditory systems in the Monodelphis opossum. In contrast to rodents, the lateral dorsal and lateral posterior nuclei in the opossum are poorly differentiated cytoarchitectonically.
Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, 02-093 Warsaw, 3 Pasteur Street, Poland.
Seweryn
S
Olkowicz
Kris
K
Turlejski
Katarzyna
K
Bartkowska
Ewa
E
Wielkopolska
Rouzanna L
RL
Djavadian
D000110:Acetylcholinesterase / Q000378:metabolism; D000818:Animals; D020643:Anterior Thalamic Nuclei / Q000033:anatomy & histology / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001931:Brain Mapping; D064032:Calbindin 2; D005260:Female; D005829:Geniculate Bodies / Q000033:anatomy & histology / Q000378:metabolism; D006651:Histocytochemistry; D007150:Immunohistochemistry; D020647:Lateral Thalamic Nuclei / Q000033:anatomy & histology / Q000378:metabolism; D008297:Male; D051379:Mice; D048309:Monodelphis / Q000033:anatomy & histology* / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D010320:Parvalbumins / Q000378:metabolism; D010802:Phylogeny; D064030:S100 Calcium Binding Protein G / Q000378:metabolism; D013045:Species Specificity; D013787:Thalamic Nuclei / Q000033:anatomy & histology* / Q000378:metabolism
D015415:Biomarkers; C576511:Calb2 protein, mouse; D064032:Calbindin 2; D010320:Parvalbumins; D064030:S100 Calcium Binding Protein G; D000110:Acetylcholinesterase