NF-kappaB inhibitory activity of compounds isolated from Cantharellus cibarius.
The inhibitory activities of the extracts of Cantharellus cibarius and isolated compounds were investigated in an enzyme-based ELISA NF-kappaB assay. Of the tested compounds, ergosterol, ergosterol peroxide and cerevisterol were noted to have the most potent inhibition of NF-kappaB activation. The ability of the active metabolites to inhibit the NF-kappaB translocation from the cytoplasm to the nucleus was assessed using a cell-based NF-kappaB assay. The isolated compounds were elucidated through the analysis of various spectroscopic methods.
Division of Pharmacy Practice and Administration, College of Pharmacy, The Ohio State University, Columbus, OH 43210, USA.
Jeong Ah
JA
Kim
David
D
Tay
Esperanza Carcache
EC
de Blanco
D001487:Basidiomycota / Q000737:chemistry*; D004875:Ergosterol / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D019084:Fluorescent Antibody Technique, Indirect; D006367:HeLa Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D016328:NF-kappa B / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010840:Phytosterols / Q000302:isolation & purification / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*
D016328:NF-kappa B; D010840:Phytosterols; D010936:Plant Extracts; C006954:cerevisterol; C036071:ergosterol-5,8-peroxide; D004875:Ergosterol