Gene amplification is a poor prognostic factor in anaplastic oligodendrogliomas.
Various gene amplifications have been observed in gliomas. Prognostic-genomic correlations testing simultaneously all these amplified genes have never been conducted in anaplastic oligodendrogliomas. A set of 38 genes that have been reported to be amplified in gliomas and investigated as the main targets of amplicons were studied in a series of 52 anaplastic oligodendrogliomas using bacterial artificial chromosome-array based comparative genomic hybridization and quantitative polymerase chain reaction. Among the 38 target genes, 15 were found to be amplified in at least one tumor. Overall, 27% of anaplastic oligodendrogliomas exhibited at least one gene amplification. The most frequently amplified genes were epidermal growth factor receptor (EGFR) and cyclin-dependent kinase 4/sarcoma amplified sequence (CDK4/SAS) in 17% and 8% of anaplastic oligodendrogliomas, respectively. Gene amplification and codeletion of chromosome arms 1p/19q were perfectly exclusive (p = 0.005). In uni- and multivariate analyses, gene amplification was a negative prognostic factor for progression-free survival and overall survival in anaplastic oligodendrogliomas, providing complementary information to the classic prognostic factors identified in anaplastic oligodendrogliomas (extent of surgery, KPS, and chromosome arms 1p/19q status).
Idbaih
Ahmed
A
Institut National de la Santé et de la Recherche Médicale, U711, Université Pierre et Marie Curie, Hôpital Pitié-Salpêtrière, Paris, France. ahmed.idbaih@gmail.com
Crinière
Emmanuelle
E
Marie
Yannick
Y
Rousseau
Audrey
A
Mokhtari
Karima
K
Kujas
Michèle
M
El Houfi
Younas
Y
Carpentier
Catherine
C
Paris
Sophie
S
Boisselier
Blandine
B
Laigle-Donadey
Florence
F
Thillet
Joëlle
J
Sanson
Marc
M
Hoang-Xuan
Khê
K
Delattre
Jean-Yves
JY
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001932:Brain Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D022202:Chromosomes, Artificial, Bacterial; D005260:Female; D005784:Gene Amplification; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D009693:Nucleic Acid Hybridization; D009837:Oligodendroglioma / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D011379:Prognosis; D020133:Reverse Transcriptase Polymerase Chain Reaction