Gene array analysis reveals a common Runx transcriptional programme controlling cell adhesion and survival.
The Runx genes are important in development and cancer, where they can act either as oncogenes or tumour suppressors. We compared the effects of ectopic Runx expression in established fibroblasts, where all three genes produce an indistinguishable phenotype entailing epithelioid morphology and increased cell survival under stress conditions. Gene array analysis revealed a strongly overlapping transcriptional signature, with no examples of opposing regulation of the same target gene. A common set of 50 highly regulated genes was identified after further filtering on regulation by inducible RUNX1-ER. This set revealed a strong bias toward genes with annotated roles in cancer and development, and a preponderance of targets encoding extracellular or surface proteins, reflecting the marked effects of Runx on cell adhesion. Furthermore, in silico prediction of resistance to glucocorticoid growth inhibition was confirmed in fibroblasts and lymphoid cells expressing ectopic Runx. The effects of fibroblast expression of common RUNX1 fusion oncoproteins (RUNX1-ETO, TEL-RUNX1 and CBFB-MYH11) were also tested. Although two direct Runx activation target genes were repressed (Ncam1 and Rgc32), the fusion proteins appeared to disrupt the regulation of downregulated targets (Cebpd, Id2 and Rgs2) rather than impose constitutive repression. These results elucidate the oncogenic potential of the Runx family and reveal novel targets for therapeutic inhibition.
Faculty of Veterinary Medicine, Molecular Oncology Laboratory,Institute of Comparative Medicine, University of Glasgow, Glasgow, Scotland. S.Wotton@vet.gla.ac.uk
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Wotton
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Terry
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Kilbey
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Jenkins
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Herzyk
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Cameron
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JC
Neil
D000818:Animals; D017209:Apoptosis; D002448:Cell Adhesion / Q000235:genetics*; D002470:Cell Survival / Q000235:genetics; D050657:Core Binding Factor alpha Subunits / Q000235:genetics* / Q000378:metabolism*; D005347:Fibroblasts / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D051379:Mice; D041681:NIH 3T3 Cells; D020411:Oligonucleotide Array Sequence Analysis; D015513:Oncogene Proteins / Q000235:genetics* / Q000378:metabolism*; D014158:Transcription, Genetic
D050657:Core Binding Factor alpha Subunits; D015513:Oncogene Proteins