Thermodynamic analysis of denaturant-induced unfolding of HodC69S protein supports a three-state mechanism.
Thermodynamic stability parameters and the equilibrium unfolding mechanism of His 6HodC69S, a mutant of 1 H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (Hod) having a Cys to Ser exchange at position 69 and an N-terminal hexahistidine tag (His 6HodC69S), have been derived from isothermal unfolding studies using guanidine hydrochloride (GdnHCl) or urea as denaturants. The conformational changes were monitored by following changes in circular dichroism (CD), fluorescence, and dynamic light scattering (DLS), and the resulting transition curves were analyzed on the basis of a sequential three-state model N = I = D. The structural changes have been correlated to catalytic activity, and the contribution to stability of the disulfide bond between residues C37 and C184 in the native protein has been established. A prominent result of the present study is the finding that, independent of the method used for denaturing the protein, the unfolding mechanism always comprises three states which can be characterized by, within error limits, identical sets of thermodynamic parameters. Apparent deviations from three-state unfolding can be rationalized by the inability of a spectroscopic probe to discriminate clearly between native, intermediate, and unfolded ensembles. This was the case for the CD-monitored urea unfolding curve.
Institut für Physikalische Chemie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 30, 48149 Münster, Germany.
Kristian
K
Boehm
Jessica
J
Guddorf
Alexander
A
Albers
Tadashi
T
Kamiyama
Susanne
S
Fetzner
H-J
HJ
Hinz
D001173:Arthrobacter / Q000187:drug effects / Q000201:enzymology* / Q000528:radiation effects; D002942:Circular Dichroism; D049308:Dioxygenases / Q000737:chemistry* / Q000378:metabolism*; D004220:Disulfides / Q000737:chemistry; D008027:Light; D050505:Mutant Proteins / Q000737:chemistry* / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects / Q000528:radiation effects; D011489:Protein Denaturation / Q000187:drug effects / Q000528:radiation effects; D017510:Protein Folding; D012542:Scattering, Radiation; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics; D014508:Urea / Q000494:pharmacology*
D004220:Disulfides; D050505:Mutant Proteins; D014508:Urea; C101932:1H-3-hydroxy-4-oxoquinaldine 2,4-dioxygenase (decyclizing, CO-forming); D049308:Dioxygenases