Molecular recognition of a DNA:RNA hybrid: sub-nanomolar binding by a neomycin-methidium conjugate.
A novel neomycin-methidium conjugate was synthesized. The covalent linkage of the aminoglycoside to an intercalator, a derivative of ethidium bromide, results in a new conjugate capable of selective recognition of the DNA:RNA hybrid duplex. Spectroscopic methods: UV, CD, fluorescence, and calorimetric techniques: DSC and ITC were used to characterize the sub-nanomolar binding displayed by the conjugate for the DNA:RNA hybrid duplex, poly(dA):poly(rU).
Laboratory of Medicinal Chemistry, Department of Chemistry, Clemson University, Clemson, SC 29634, USA.
Nicholas N
NN
Shaw
Hongjuan
H
Xi
Dev P
DP
Arya
D000617:Aminoglycosides / Q000737:chemistry; D002151:Calorimetry; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007364:Intercalating Agents / Q000494:pharmacology; D008956:Models, Chemical; D008968:Molecular Conformation; D009355:Neomycin / Q000737:chemistry*; D009690:Nucleic Acid Conformation; D010617:Phenanthridines / Q000737:chemistry*; D012313:RNA / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D013056:Spectrophotometry, Ultraviolet / Q000379:methods; D013696:Temperature; D013816:Thermodynamics
D000617:Aminoglycosides; D007364:Intercalating Agents; D010617:Phenanthridines; D012313:RNA; D004247:DNA; D009355:Neomycin