Spatial distribution of cadmium in leaves of metal hyperaccumulating Thlaspi praecox using micro-PIXE.
* Localization of cadmium (Cd) and other elements was studied in the leaves of the field-collected cadmium/zinc (Cd/Zn) hyperaccumulator Thlaspi praecox from an area polluted with heavy metals near a lead mine and smelter in Slovenia, using micro-PIXE (proton-induced X-ray emission). * The samples were prepared using cryofixation. Quantitative elemental maps and average concentrations in whole-leaf cross-sections and selected tissues were obtained. * Cd was preferentially localized in the lower epidermis (820 microg g(-1) DW), vascular bundles and upper epidermis, whereas about twice the lower concentrations were found in the mesophyll. * Taking into account the large volume of the mesophyll compared with the epidermis, the mesophyll is indicated as a relatively large pool of Cd, possibly involved in Cd detoxification/dilution at the tissue and cellular level.
Vogel-Mikuš
Katarina
K
Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, SI-1000 Ljubljana, Slovenia.
Regvar
Marjana
M
Department of Biology, Biotechnical Faculty, University of Ljubljana, Večna pot 111, SI-1000 Ljubljana, Slovenia.
Mesjasz-Przybyłowicz
Jolanta
J
Materials Research Group, iThemba LABS, PO Box 722, Somerset West 7129, South Africa.
Przybyłowicz
Wojciech J
WJ
Materials Research Group, iThemba LABS, PO Box 722, Somerset West 7129, South Africa.|Faculty of Physics and Applied Computer Science, AGH University of Science and Technology, 30-059 Kraków, Poland.
Simčič
Jure
J
Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.
Pelicon
Primož
P
Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.
Budnar
Miloš
M
Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia.
D002104:Cadmium / Q000032:analysis* / Q000378:metabolism; D004785:Environmental Pollutants / Q000032:analysis* / Q000378:metabolism; D007854:Lead; D008906:Mining; D018515:Plant Leaves / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D017524:Slovenia; D013052:Spectrometry, X-Ray Emission; D031227:Thlaspi / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
D004785:Environmental Pollutants; D002104:Cadmium; D007854:Lead