Evaluation of renal oxygenation in rat by using R2' at 3-T magnetic resonance: initial observation.
OBJECTIVE
We sought to initially evaluate the feasibility of R2' on a 3-T magnetic resonance (MR) scanner for assessment of renal oxygenation changes following administration of furosemide in rats.


METHODS
Eight intact male Wistar rats were involved in experimental group. The experiment was performed at a 3-T MR scanner using a multiple gradient-echo (mGRE) sequence for R2* map and a multiecho fast spin-echo (FSE) sequence for R2 map. R2' values of cortex and medulla were calculated using the equation R2* = R2 + R2'. The values of R2 and R2* were measured and R2' was calculated before and after administration of furosemide, and the changes (delta values) were calculated.


RESULTS
Both R2* and R2 values decreased significantly after administration of furosemide (P < .001) in both the cortex and medulla. DeltaR2* in the medulla was significantly higher than in the cortex (P < .05). DeltaR2 was not significantly different between the cortex and medulla (P > .05). The baseline R2' value was 12.13 +/- 0.59 1/s in the cortex and 19.52 +/- 3.44 1/s in the medulla. R2' value decreased significantly in the medulla after administration of furosemide (P < .05), but there was no significant difference in the cortex before and after administration of furosemide (P > .05).


CONCLUSIONS
R2' may be more appropriate than R2* to indicate the change of oxygenation after administration of furosemide in intact rats at 3-T MR. Further studies are needed for both intact animals and experimental models in comparison with non-MR imaging methods to validate this initial observation.
Yang
Xuedong
X
Department of Radiology, Peking, First Hospital and Functional Imaging Center, Advanced Academy of Interdisciplinary Sciences, Peking University, No. 8, Xishiku Street, Xicheng District, Beijing 100034, China.
Cao
Ju
J
Wang
Xiaoying
X
Li
Xiaomei
X
Xu
Yufeng
Y
Jiang
Xuexiang
X
D000818:Animals; D003287:Contrast Media / Q000008:administration & dosage; D005665:Furosemide / Q000008:administration & dosage; D007668:Kidney / Q000098:blood supply*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D010100:Oxygen / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar
D003287:Contrast Media; D005665:Furosemide; D010100:Oxygen