Excretory-secretory products of larval Fasciola hepatica investigated using a two-dimensional proteomic approach.
So far, very few secreted proteins from trematodes have been characterized, although their role in the mechanisms that allow the parasite to escape host's immune response have been largely documented. Here we performed a proteomic analysis of excretory-secretory proteins from the intra-molluscan larval stages of Fasciola hepatica. We identified two antioxidative enzymes: a Cu/Zn-superoxide dismutase (Cu/Zn SOD) and a thioredoxin (TRX) previously characterized in ES products from adult stages. These results support the importance of parasite detoxication of reactive oxygen species in invertebrate hosts, and raise the question of the possible conservation of major immune evasion effectors across trematode developmental life-stages.
Gourbal
Benjamin E F
BE
UMR 5244 CNRS-EPHE-UPVD, Parasitologie Fonctionnelle et Evolutive, Université de Perpignan, 52 Ave Paul Alduy, 66860 Perpignan Cedex, France.
Guillou
François
F
Mitta
Guillaume
G
Sibille
Pierre
P
Thèron
André
A
Pointier
Jean-Pierre
JP
Coustau
Christine
C
D000818:Animals; D015180:Electrophoresis, Gel, Two-Dimensional; D005210:Fasciola hepatica / Q000737:chemistry*; D007814:Larva / Q000737:chemistry; D020543:Proteome / Q000032:analysis*; D013482:Superoxide Dismutase / Q000302:isolation & purification; D013879:Thioredoxins / Q000302:isolation & purification
D020543:Proteome; D013879:Thioredoxins; D013482:Superoxide Dismutase