Selective enrichment of cysteine-containing peptides using SPDP-functionalized superparamagnetic Fe(3)O(4)@SiO(2) nanoparticles: application to comprehensive proteomic profiling.
Superparamagnetic Fe(3)O(4)@SiO(2) core-shell nanoparticles (ca. 30 nm diameter), which are surface-modified with a thiol-specific functional group, exhibit superb capturing efficiency toward cysteinyl peptides without contamination from nonspecifically interacting peptides, as clearly evidenced through LC/MS/MS analysis.
Palani
Arudra
A
Department of Chemistry and Center for Electro- & Photo-Responsive Molecules, Korea University, Seoul, South Korea.
Lee
Je-Sun
JS
Huh
Jiyoung
J
Kim
Minsik
M
Lee
Yeon-Ji
YJ
Chang
Jeong Ho
JH
Lee
Kwangyeol
K
Lee
Sang-Won
SW
D002853:Chromatography, Liquid; D003545:Cysteine / Q000032:analysis*; D052203:Ferrosoferric Oxide; D007202:Indicators and Reagents; D053758:Nanoparticles; D010455:Peptides / Q000032:analysis* / Q000302:isolation & purification; D040901:Proteomics / Q000379:methods*; D012822:Silicon Dioxide; D013388:Succinimides; D053719:Tandem Mass Spectrometry
D007202:Indicators and Reagents; D010455:Peptides; D013388:Succinimides; C018151:N-succinimidyl 3-(2-pyridyldithio)propionate; D012822:Silicon Dioxide; D003545:Cysteine; D052203:Ferrosoferric Oxide