Antitumor effect of a transducible fusogenic peptide releasing multiple proapoptotic peptides by caspase-3.
We have designed a novel peptide, TK3, composed of three functional domains, a protein transduction domain, a TAT followed by three tandem repeats of a proapoptotic peptide, and a caspase-3 cleavage site, (KLAKLAK)(2)-DEVD. TK3 was able to transduce into cells and then activate caspase-3, which in turn cleaved TK3 to release additional (KLAKLAK)(2) peptides. (KLAKLAK)(2) was well transduced by TAT into tumor cells and was able to induce apoptosis in vitro and in vivo. TK3 also induced apoptosis and inhibited angiogenesis in endothelial cells. Further, direct injection of TK3 into established B16F10 melanoma tumors in C57BL/6 mice resulted in almost complete inhibition of the tumor growth. These results suggest that TK3 could be beneficial for the treatment of accessible tumors and used as an adjuvant for cancer therapy.
Kwon
Mi-Kyung
MK
Department of Biochemistry and Cell Biology, Cell and Matrix Research Institute, School of Medicine, Kyungpook National University, 101 Dongin-2ga, Jung-gu, Daegu 700-422, Korea.
Nam
Ju-Ock
JO
Park
Rang-Woon
RW
Lee
Byung-Heon
BH
Park
Jae-Yong
JY
Byun
Young-Ro
YR
Kim
Sang-Yoon
SY
Kwon
Ick-Chan
IC
Kim
In-San
IS
D000595:Amino Acid Sequence; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D016466:CHO Cells; D053148:Caspase 3 / Q000378:metabolism*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D006224:Cricetinae; D003412:Cricetulus; D042783:Endothelial Cells / Q000187:drug effects / Q000473:pathology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D009389:Neovascularization, Pathologic / Q000473:pathology; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D013937:Thymidine Kinase / Q000378:metabolism; D014161:Transduction, Genetic
D000970:Antineoplastic Agents; D010455:Peptides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D013937:Thymidine Kinase; D053148:Caspase 3