Estimation of free copper ion concentrations in blood serum using T(1) relaxation rates.
The water proton relaxation rate constant R(1)=1/T(1) (at 60 MHz) of blood serum is substantially increased by the presence of free Cu2+ ions at concentrations above normal physiological levels. Addition of chelating agents to serum containing paramagnetic Cu2+ nulls this effect. This was demonstrated by looking at the effect of adding a chelating agent-D-penicillamine (D-PEN) to CuSO4 and CuCl2 aqueous solutions as well as to rabbit blood serum. We propose that the measurement of water proton spin-lattice relaxation rate constants before and after chelation may be used as an alternative approach for monitoring the presence of free copper ions in blood serum. This method may be used in the diagnosis of some diseases (leukaemia, liver diseases and particularly Wilson's disease) because, in contrast to conventional methods like spectrophotometry which records the total number of both bound and free ions, the proton relaxation technique is sensitive solely to free paramagnetic ions dissolved in blood serum. The change in R(1) upon chelation was found to be less than 0.06 s(-1) for serum from healthy subjects but greater than 0.06 s(-1) for serum from untreated Wilson's patients.
Institute of Physics, Jagellonian University, Reymonta 4, 30059 Krakow, Poland. BB@netmail.if.uj.edu.pl
Barbara
B
Blicharska
Magdalena
M
Witek
Maria
M
Fornal
Alex L
AL
Mackay
D000465:Algorithms; D001774:Blood Chemical Analysis / Q000379:methods; D002614:Chelating Agents / Q000737:chemistry*; D003300:Copper / Q000097:blood* / Q000737:chemistry*; D006527:Hepatolenticular Degeneration / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D007477:Ions / Q000097:blood / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D002614:Chelating Agents; D007477:Ions; D003300:Copper