New finding of Giardia intestinalis (Eukaryote, Metamonad) in Old World archaeological site using immunofluorescence and enzyme-linked immunosorbent assays.
In this study, nine organic sediment samples from a medieval archaeological site at Pineuilh, France, were examined for Giardia intestinalis using two commercially available immunological kits [enzyme-linked immuno sorbent and immunofluorescence (IFA) assays]. Both techniques detected G. intestinalis in one sample, dated to 1,000 Anno Domini. This is the first time IFA was successfully used to detect protozoa in Old World archaeological samples. Such immunological techniques offer important perspectives concerning ancient protozoa detection and identification.
Le Bailly
Matthieu
M
Laboratoire de Paléoparasitologie, UFR de Pharmacie, Université de Reims, Reims, France. matthieu.lebailly@univ-reims.fr
Gonçalves
Marcelo Lc
ML
Harter-Lailheugue
Stéphanie
S
Prodéo
Frédéric
F
Araujo
Adauto
A
Bouchet
Françoise
F
D000818:Animals; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000469:parasitology; D005455:Fluorescent Antibody Technique; D005602:France; D005872:Giardia / Q000302:isolation & purification*; D006801:Humans; D010164:Paleopathology