Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements.
Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.
Laboratory for Neuronal Circuit Dynamics, RIKEN Brain Science Institute, Wako-City, Saitama, Japan.
Alicia
A
Lundby
Hiroki
H
Mutoh
Dimitar
D
Dimitrov
Walther
W
Akemann
Thomas
T
Knöpfel
D000818:Animals; D005453:Fluorescence; D005818:Genetic Engineering; D015640:Ion Channel Gating; D016716:PC12 Cells; D015221:Potassium Channels / Q000737:chemistry / Q000502:physiology*; D011487:Protein Conformation; D051381:Rats
D015221:Potassium Channels