Stimulation of osteoblasts with Emdogain increases the expression of specific mineralization markers.
OBJECTIVE
The purpose of this study was to determine the effects of enamel matrix derivative on mRNA expression of markers related to periodontal healing.


METHODS
Murine osteoprogenitor cells (MC3T3-E1) were grown for 12 and 16 days in mineralization media and stimulated with 100 microg/mL Emdogain (EMD). Cell cultures treated with 2% and 10% fetal calf serum (FCS) served as control. The mRNA expression of bone sialoprotein (BSP), osteopontin (OPN), and runt-related protein 2 (Runx2) was analyzed by real-time polymerase chain reaction. One-way analysis of variance was used for statistical analysis.


RESULTS
Stimulation with EMD significantly (P < .01) enhanced mRNA expression of BSP up to 13.9-fold and of OPN up to 3.2-fold at day 16 compared with the 2% FCS control. The expression of mRNA for transcription factor Runx2 was not significantly changed.


CONCLUSIONS
The beneficial effects seen in periodontal regeneration after treatment with EMD may be related to an increase of the mineralization markers BSP and OPN at mRNA level.
Department of Operative Dentistry and Periodontology, Ludwig-Maximilians-University, Munich, Germany. pweis@dent.med.uni-muenchen.de
Peggy
P
Weishaupt
Jean-Pierre
JP
Bernimoulin
Philip
P
Trackman
Stefan
S
Hägewald
D016475:3T3 Cells; D000818:Animals; D002113:Calcification, Physiologic / Q000187:drug effects*; D050659:Core Binding Factor Alpha 1 Subunit / Q000032:analysis / Q000187:drug effects; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000235:genetics; D058574:Integrin-Binding Sialoprotein; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects*; D053495:Osteopontin / Q000032:analysis / Q000187:drug effects; D012333:RNA, Messenger / Q000187:drug effects; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000032:analysis / Q000187:drug effects
D050659:Core Binding Factor Alpha 1 Subunit; D003746:Dental Enamel Proteins; C546872:Ibsp protein, mouse; D058574:Integrin-Binding Sialoprotein; D012333:RNA, Messenger; C493716:Runx2 protein, mouse; D012795:Sialoglycoproteins; C506896:Spp1 protein, mouse; C109069:enamel matrix proteins; D053495:Osteopontin