Molecular epidemiology of primary human cytomegalovirus infection in pregnant women and their families.
The source of human cytomegalovirus (HCMV) infection was investigated in 29 pregnant women with primary HCMV infection by comparing DNA sequences of UL146, UL144 and a portion of UL55 gene of HCMV strains circulating within each family. Thirteen families were identified in which the pregnant woman, the husband and/or a child were shedding HCMV. In three of these families, both the woman and the husband suffered from a concomitant primary HCMV infection. Phylogenetic analysis of UL146, UL144, and UL55 genes indicated that strains circulating within each family were identical, whereas strains from different families appeared to be distinct. However, identical UL146, UL144, and UL55 DNA sequences were observed sporadically among unrelated strains. A child rather than the husband was the virus source for the great majority of pregnant women. No association was observed between UL144 polymorphisms and intrauterine transmission.
Revello
Maria Grazia
MG
Servizio di Virologia, Fondazione Istituto di Ricovero e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy. mg.revello@smatteo.pv.it
Campanini
Giulia
G
Piralla
Antonio
A
Furione
Milena
M
Percivalle
Elena
E
Zavattoni
Maurizio
M
Gerna
Giuseppe
G
D000328:Adult; D019743:Chemokines, CXC / Q000235:genetics; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D003586:Cytomegalovirus Infections / Q000453:epidemiology / Q000635:transmission / Q000821:virology; D005191:Family Characteristics; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008562:Membrane Glycoproteins / Q000235:genetics; D017720:Molecular Epidemiology; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000453:epidemiology / Q000821:virology; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
D019743:Chemokines, CXC; D008562:Membrane Glycoproteins; C119624:UL144 ORF protein, Human herpesvirus 5; D014764:Viral Proteins; C121017:viral chemokine CXC-1, Cytomegalovirus