Molecular cloning of a cDNA encoding atypical antimicrobial and cytotoxic Brevinin-1Ja from the skin of the Japanese brown frog, Rana japonica.
Antimicrobial peptides (AMPs) are important components of the host innate defense system against pathogenic microbial invasion in many organisms. In the present study, we cloned by RT-PCR a cDNA from total RNA prepared from the skin of the Japanese brown frog Rana japonica. The cDNA directs the synthesis of a novel, non-C-terminally alpha-amidated peptide composed of 21 amino acid residues (FLGSLIGAAIPAIKQLLGLKK). The putative peptide showed limited sequence similarity to atypical acyclic brevinin-1OK family AMPs originally isolated from the skin of the Ryukyu brown frog (R. okinavana), which lacks the COOH-terminal cyclic domain commonly observed in typical brevinin-1 groups, but that contains a C-terminally alpha-amidated residue. Although it is unclear whether the peptide, designated brevinin-1Ja, is produced in R. japonica skin, a synthetic replicate of the peptide showed differential growth-inhibiting activity against the Gram-positive bacterium Staphylococcus aureus and Gram-negative bacterium Escherichia coli (minimal inhibitory concentrations: 15 microM and 119 microM, respectively), and produced cell death of mammalian COS7 cells (LD50=28 microM).
Koyama
Takumi
T
Department of Biology, Faculty of Science, Toho University, Chiba, Japan.
Iwamuro
Shawichi
S
D000595:Amino Acid Sequence; D029845:Amphibian Proteins / Q000235:genetics*; D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics*; D000970:Antineoplastic Agents / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics*; D008969:Molecular Sequence Data; D011898:Ranidae / Q000235:genetics*; D012867:Skin / Q000378:metabolism*
D029845:Amphibian Proteins; D023181:Antimicrobial Cationic Peptides; D000970:Antineoplastic Agents; D018076:DNA, Complementary; C078413:brevinin-1 protein, Rana