Petalonia improves glucose homeostasis in streptozotocin-induced diabetic mice.
The anti-diabetic potential of Petalonia binghamiae extract (PBE) was evaluated in vivo. Dietary administration of PBE to streptozotocin (STZ)-induced diabetic mice significantly lowered blood glucose levels and improved glucose tolerance. The mode of action by which PBE attenuated diabetes was investigated in vitro using 3T3-L1 cells. PBE treatment stimulated 3T3-L1 adipocyte differentiation as evidenced by increased triglyceride accumulation. At the molecular level, peroxisome proliferator-activated receptor gamma (PPARgamma) and terminal marker protein aP2, as well as the mRNA of GLUT4 were up-regulated by PBE. In mature adipocytes, PBE significantly stimulated the uptake of glucose and the expression of insulin receptor substrate-1 (IRS-1). Furthermore, PBE increased PPARgamma luciferase reporter gene activity in COS-1 cells. Taken together, these results suggest that the in vivo anti-diabetic effect of PBE is mediated by both insulin-like and insulin-sensitizing actions in adipocytes.
Kang
Seong-Il
SI
Department of Life Science, Cheju National University, 66 Jejudaehakno, Ara-1 Dong, Jejusi, Jeju 690-756, Republic of Korea.
Jin
Young-Jun
YJ
Ko
Hee-Chul
HC
Choi
Soo-Youn
SY
Hwang
Joon-Ho
JH
Whang
Ilson
I
Kim
Moo-Han
MH
Shin
Hye-Sun
HS
Jeong
Hyung-Bok
HB
Kim
Se-Jae
SJ
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000473:pathology; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D002454:Cell Differentiation / Q000187:drug effects; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism* / Q000473:pathology; D005951:Glucose Tolerance Test; D006706:Homeostasis / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D051379:Mice; D008813:Mice, Inbred ICR; D047495:PPAR gamma / Q000378:metabolism; D000459:Phaeophyceae / Q000737:chemistry*; D015533:Transcriptional Activation / Q000187:drug effects
D001786:Blood Glucose; D007004:Hypoglycemic Agents; D047495:PPAR gamma