Stimulatory effect of Ceriops tagal on hexose uptake in L6 muscle cells in culture.
The ethanolic extracts of a mangrove plant Ceriops tagal (Family Rhizophoraceae) and its sequential fractions thereof were studied for their effect on 3H-2-deoxyglucose uptake by L6 rat muscle cells cultured to the myotube stage. Among these, the n-hexane soluble fraction of ethanolic extract of the leaves of C. tagal enhanced 3H-2-deoxyglucose uptake even at 2 microg mL(-1) concentration with half maximum activity at 10 microg mL(-1), comparable with insulin (1 microM) and metformin (400 microM). This enhancement in glucose uptake was found to be insulin independent and in contrast to insulin, its effect was also prevalent in undifferentiated myoblasts. It may be concluded from the results that n-hexane soluble fraction of ethanolic extract of C. tagal have the property to stimulate the glucose uptake, which might be a useful source for the isolation of new antihyperglycemic compounds.
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Akhilesh Kumar
AK
Tamrakar
Rajesh
R
Kumar
Ramesh
R
Sharma
Anil Kumar
AK
Balapure
Vijai
V
Lakshmi
Arvind Kumar
AK
Srivastava
D000818:Animals; D002460:Cell Line; D005947:Glucose / Q000378:metabolism*; D007004:Hypoglycemic Agents / Q000032:analysis*; D018485:Muscle Fibers, Skeletal / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats; D043982:Rhizophoraceae / Q000737:chemistry*
D007004:Hypoglycemic Agents; D010936:Plant Extracts; D005947:Glucose