Secretion of human interferon-beta 1b by recombinant Lactococcus lactis.
Interferon-beta has anti-viral, anti-proliferation and multifunctional immunomodulatory activities and shows promising clinical effects for treatment of inflammatory disorders. The recombinant human interferon-beta (huIFN-beta) 1b was expressed in the food-grade lactic acid bacterium, Lactococcus lactis, using a nisin-controlled gene expression system. huIFN-beta production from recombinant strains (with and without LEISSTCDA propeptide) was approximately 21 and 7 microg l(-1), respectively. Moreover, 95% (former strain) and 88% (latter strain) of total recombinant proteins were secreted into the culture medium. The biological activities of huIFN-beta from recombinant strains revealed similar antiviral activities of 10(7) I.U. mg(-1). These results demonstrate the potential application of recombinant strains as a food grade vehicle to deliver bioactive huIFN-beta in vivo.
Zhuang
Zhong
Z
State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong 250100, People's Republic of China.
Wu
Zhi-Gang
ZG
Chen
Min
M
Wang
Peng George
PG
D015153:Blotting, Western; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D000068576:Interferon beta-1b; D016899:Interferon-beta / Q000378:metabolism*; D013294:Lactococcus lactis / Q000235:genetics*; D011994:Recombinant Proteins / Q000378:metabolism; D011995:Recombination, Genetic
D011994:Recombinant Proteins; D000068576:Interferon beta-1b; D016899:Interferon-beta