Determination of adrenal steroids by microfluidic chip using micellar electrokinetic chromatography.
This paper describes a sensitive and convenient method to separate progesterone, 17alpha-hydroxy progesterone, cortexolone, hydrocortisone and cortisone, all of which are steroids and have similar structures, using microfluidic chip-based technology with UV detection at 252 nm. We successfully obtained high-speed separation of the five steroids within 70 s in optimized microfluidic controls and micellar electrokinetic chromatography (MEKC) separation conditions. Fairly good linearity with correlation coefficient of over 0.98 from 10 or 20 to 100 mg/l steroid chemicals was obtained. The limits of detection obtained at a signal to noise ratio of 3 were from 3.89 to 7.80 mg/l. The values of the relative standard deviation (RSD) were 0.98-1.34% for repetitive injection (n = 12) and the intraday and interday RSDs were below 6%. The highly stable response reflected the feasibility of this method.
Department of Chemistry, Changzhi College, 73 Chengbei East Street, Changzhi, 046011 Shanxi, China.
Shuanglong
S
Shen
Yan
Y
Li
Shin-ichi
S
Wakida
Sahori
S
Takeda
D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D003348:Cortisone / Q000032:analysis; D003350:Cortodoxone / Q000032:analysis; D006854:Hydrocortisone / Q000032:analysis; D044085:Microfluidics; D011374:Progesterone / Q000031:analogs & derivatives / Q000032:analysis; D015203:Reproducibility of Results; D013256:Steroids / Q000032:analysis*
D013256:Steroids; C002175:progesterone 17 alpha-hydroperoxide; D011374:Progesterone; D003348:Cortisone; D003350:Cortodoxone; D006854:Hydrocortisone