Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells.
The planar cell polarity (PCP) signaling system governs many aspects of polarized cell behavior. Here, we use an in vivo model of vertebrate mucociliary epithelial development to show that Dishevelled (Dvl) is essential for the apical positioning of basal bodies. We find that Dvl and Inturned mediate the activation of the Rho GTPase specifically at basal bodies, and that these three proteins together mediate the docking of basal bodies to the apical plasma membrane. Moreover, we find that this docking involves a Dvl-dependent association of basal bodies with membrane-bound vesicles and the vesicle-trafficking protein, Sec8. Once docked, basal bodies again require Dvl and Rho for the planar polarization that underlies directional beating of cilia. These results demonstrate previously undescribed functions for PCP signaling components and suggest that a common signaling apparatus governs both apical docking and planar polarization of basal bodies.
Park
Tae Joo
TJ
Department of Molecular Cell and Developmental Biology & Institute for Cellular and Molecular Biology, University of Texas, Austin, Texas 78712, USA.
Mitchell
Brian J
BJ
Abitua
Philip B
PB
Kintner
Chris
C
Wallingford
John B
JB
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000818:Animals; D030801:Animals, Genetically Modified; D002450:Cell Communication / Q000235:genetics*; D002465:Cell Movement / Q000235:genetics; D016764:Cell Polarity / Q000235:genetics*; D002923:Cilia / Q000378:metabolism / Q000502:physiology; D017124:Conserved Sequence; D000072261:Dishevelled Proteins; D004625:Embryo, Nonmammalian; D004847:Epithelial Cells / Q000378:metabolism*; D005260:Female; D008954:Models, Biological; D038081:Organogenesis / Q000235:genetics; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D017434:Protein Structure, Tertiary; D021381:Protein Transport / Q000235:genetics; D033921:Vesicular Transport Proteins / Q000378:metabolism; D029867:Xenopus Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D014982:Xenopus laevis / Q000196:embryology; D020741:rho GTP-Binding Proteins / Q000235:genetics / Q000502:physiology
D048868:Adaptor Proteins, Signal Transducing; C000606586:DVL1 protein, Xenopus; D000072261:Dishevelled Proteins; D010750:Phosphoproteins; C491770:Rnd1 protein, Xenopus; C530225:Sec8 protein, Xenopus; D033921:Vesicular Transport Proteins; D029867:Xenopus Proteins; D020741:rho GTP-Binding Proteins