Coimmobilization of substrate and biocatalyst: A method for bioconversion of poorly soluble substances in water milieu.
Coimmobilization of biocatalyst and substrate was studied as a method to increase the conversion rate in systems with substrates of extremely low solubility in water. The system studied was the conversion of hydrocortisone to prednisolone by Arthrobacter simplex. As a matrix for coimmobilization, alginate turned out to be superior to agar and agarose. After the reaction was complete, the beads were solubilized, andthe cells recovered for reuse, by centrifugation, whereas the prednisolone was extracted from the supernatant.
Department of Biotechnology, Chemical Center, University of Lund, P.O. Box 124, S-221-00 Lund, Sweden.
R
R
Kaul
P
P
Adlercreutz
B
B
Mattiasson