Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli.
We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.
Hill
Joshua
J
Department of Pediatrics, Children's Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, PA 15213, USA.
Beriwal
Shilpa
S
Chandra
Ishwad
I
Paul
Vinod K
VK
Kapil
Aarti
A
Singh
Tripti
T
Wadowsky
Robert M
RM
Singh
Vinita
V
Goyal
Ankur
A
Jahnukainen
Timo
T
Johnson
James R
JR
Tarr
Phillip I
PI
Vats
Abhay
A
D001431:Bacteriological Techniques / Q000379:methods*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014556:Urine / Q000382:microbiology
D017931:DNA Primers