Lipidomic analysis of Toxoplasma gondii tachyzoites rhoptries: further insights into the role of cholesterol.
Rhoptries are secretory organelles involved in the virulence of the human pathogen Toxoplasma gondii. In the present study we have used HPLC and capillary GLC to isolate and quantify lipids from whole Toxoplasma cells and their purified rhoptries. This comparative lipidomic analysis revealed an enrichment of cholesterol, sphingomyelin and, most of all, saturated fatty acids in the rhoptries. These lipids are known, when present in membranes, to contribute to their rigidity and, interestingly, fluorescence anisotropy measurements confirmed that rhoptry-derived membranes have a lower fluidity than membranes from whole T. gondii cells. Moreover, although rhoptries were initially thought to be highly enriched in cholesterol, we demonstrated that cholesterol is present in lower proportions, and we have provided additional evidence towards a lack of involvement of rhoptry cholesterol in the process of host-cell invasion by the parasite. Indeed, depleting the cholesterol content of the parasites did not prevent the secretion of protein-containing rhoptry-derived vesicles and the parasites could still establish a structure called the moving junction, which is necessary for invasion. Instead, the crucial role of host cholesterol for invasion, which has already been demonstrated [Coppens and Joiner (2003) Mol. Biol. Cell 14, 3804-3820], might be explained by the need of a cholesterol-rich region of the host cell we could visualize at the point of contact with the attached parasite, in conditions where parasite motility was blocked.
Besteiro
Sébastien
S
Laboratoire de Dynamique des Interactions Membranaires Normales et Pathologiques (DIMNP), Universités de Montpellier II et I, Centre National de la Recherche Scientifique (CNRS), UMR 5235, Montpellier, F-34095, France. sebastien.besteiro@univ-montp2.fr
Bertrand-Michel
Justine
J
Lebrun
Maryse
M
Vial
Henri
H
Dubremetz
Jean-François
JF
D000818:Animals; D002784:Cholesterol / Q000502:physiology*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D005227:Fatty Acids / Q000032:analysis; D005347:Fibroblasts / Q000469:parasitology; D006790:Host-Parasite Interactions / Q000502:physiology*; D006801:Humans; D008055:Lipids / Q000032:analysis*; D008560:Membrane Fluidity; D021962:Membrane Microdomains / Q000502:physiology; D015388:Organelles / Q000737:chemistry*; D014122:Toxoplasma / Q000737:chemistry* / Q000472:pathogenicity / Q000648:ultrastructure*
D005227:Fatty Acids; D008055:Lipids; D002784:Cholesterol