The neuropeptide Y (NPY) Y2 receptors are largely dimeric in the kidney, but monomeric in the forebrain.
The neuropeptide Y(NPY) Y2 receptors are detected largely as dimers in the clonal expressions in CHO cells and in particulates from rabbit kidney cortex. However, in two areas of the forebrain (rat or rabbit piriform cortex and hypothalamus), these receptors are found mainly as monomers. Evidence is presented that this difference relates to large levels of G proteins containing the Gi alpha -subunit in the forebrain areas. The predominant monomeric status of these Y2 receptors should also be physiologically linked to large synaptic inputs of the agonist NPY. The rabbit kidney and the human CHO cell-expressed Y2 dimers are converted by agonists to monomers in vitro at a similar rate in the presence of divalent cations.
Department of Pharmacology, University of Tennessee Health Science Center, Memphis, Tennessee 38163, USA. stevenleonardparker@msn.com
S L
SL
Parker
M S
MS
Parker
A M
AM
Estes
Y Y
YY
Wong
R
R
Sah
T
T
Sweatman
E A
EA
Park
A
A
Balasubramaniam
F R
FR
Sallee
D000818:Animals; D016466:CHO Cells; D002413:Cations, Divalent / Q000494:pharmacology; D006224:Cricetinae; D003412:Cricetulus; D019281:Dimerization; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000378:metabolism; D016244:Guanosine 5'-O-(3-Thiotriphosphate) / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism*; D008297:Male; D016548:Prosencephalon / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D017476:Receptors, Neuropeptide Y / Q000819:agonists / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism
D002413:Cations, Divalent; D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor; D016244:Guanosine 5'-O-(3-Thiotriphosphate); D019206:GTP-Binding Protein alpha Subunits, Gi-Go