Tetrazolium salts for MIC determination in microplates: why? Which salt to select? How?
We reported evaluation of a colorimetric MIC assessment for routine susceptibility testing of non-fastidious bacteria, with addition of growth indicators (INT and MTT). Our results made us postulate that the use of such indicators was unnecessary for MIC determination in routine microdilution method.
Grare
Marion
M
SRSMC, Nancy-University, CNRS, France.
Fontanay
Stéphane
S
Cornil
Coralie
C
Finance
Chantal
C
Duval
Raphaël Emmanuel
RE
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D003124:Colorimetry; D008826:Microbial Sensitivity Tests / Q000379:methods*; D050296:Microbial Viability / Q000187:drug effects; D013778:Tetrazolium Salts / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D013778:Tetrazolium Salts