[Genotype 1 JEV was isolated again from Liaoning Province, China, 2007].
OBJECTIVE
To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene.


METHODS
Collected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods.


RESULTS
Two new JEV strains, LNDG07-02 and LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8-88% and 97.2%, respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002.


CONCLUSIONS
Genotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning Province.
Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Wei-shan
WS
Meng
Ming-hua
MH
Li
Huan-yu
HY
Wang
Ji-bo
JB
Zhang
Yu
Y
Gao
Jun
J
Ding
Shi-hong
SH
Fu
You-gang
YG
Zhai
Zhe
Z
Chen
Jun-qiao
JQ
Gou
Yu-jun
YJ
Zhao
Guo-dong
GD
Liang
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000473:pathology / Q000821:virology; D002460:Cell Line; D002681:China / Q000453:epidemiology; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000453:epidemiology / Q000821:virology; D005260:Female; D005838:Genotype; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA
D012367:RNA, Viral