Synthetic seed production from somatic embryos of Pinus radiata.
Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for the generation of material for embryo maturation. Each developmental stage of these suspensions was characterized by microscopy and their growth phases quantified. An alginate-containing medium was used as an encapsulation method for the somatic embryos that were then germinated as artificial seeds in vitro. The protocols described in this work are both useful and reliable for industrial purposes.
Aquea
Felipe
F
Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, PO Box 114-D, Santiago, Chile.
Poupin
María Josefina
MJ
Matus
José Tomás
JT
Gebauer
Marlene
M
Medina
Consuelo
C
Arce-Johnson
Patricio
P
D003470:Culture Media; D018525:Germination; D028223:Pinus / Q000196:embryology* / Q000254:growth & development; D012639:Seeds / Q000166:cytology
D003470:Culture Media