Isolation, culture and characterization of endothelial cells from human hypertrophic scar.
Although the etiology underlying scar formation is not well understood, previous studies revealed that endothelial cells play a role in the pathogenesis of scar development. Recently, the authors developed a reliable technique to obtain endothelial cells from hypertrophic scars that involved separation of cells from the scar tissue matrix and isolation from other cell types. Using phase-contract and electron microscopy, the cells were observed to have a characteristic morphology consistent with cells of endothelial origin. The cells were further characterized as endothelial cells by assessment of endothelin (ET)-1 and intercellular adhesion molecule (ICAM) mRNA expression, and the presence of factor VIII antigen, CD34, CD31, and VE-cadherin. This isolation method provides a simple method for culturing endothelial cells from hypertrophic scar tissue and should prove useful for studying the role of endothelial cell involvement in scar formation.
Wang
Xi-Qiao
XQ
Institute of Burn, Ruijin Hospital, Jiaotong University Medical School, Shanghai, The People's Republic of China.
Liu
Ying-Kai
YK
Mao
Zhi-Gang
ZG
Qing
Chun
C
Lu
Shu-Liang
SL
Xu
Da-Zhong
DZ
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D015820:Cadherins / Q000032:analysis; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D042783:Endothelial Cells / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D019332:Endothelin-1 / Q000032:analysis; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D005169:Factor VIII / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D018189:Immunomagnetic Separation / Q000379:methods*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D000099152:Cadherin 5
D015703:Antigens, CD; D018952:Antigens, CD34; D015415:Biomarkers; D015820:Cadherins; D019332:Endothelin-1; D005169:Factor VIII; D018799:Intercellular Adhesion Molecule-1; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; D000099152:Cadherin 5