In Xenopus egg extracts, DNA replication initiates preferentially at or near asymmetric AT sequences.
Previous observations led to the conclusion that in Xenopus eggs and during early development, DNA replication initiates at regular intervals but with no apparent sequence specificity. Conversely, here, we present evidence for site-specific DNA replication origins in Xenopus egg extracts. Using lambda DNA, we show that DNA replication origins are activated in clusters in regions that contain closely spaced adenine or thymine asymmetric tracks used as preferential initiation sites. In agreement with these data, AT-rich asymmetric sequences added as competitors preferentially recruit origin recognition complexes and inhibit sperm chromatin replication by increasing interorigin spacing. We also show that the assembly of a transcription complex favors origin activity at the corresponding site without necessarily eliminating the other origins. Thus, although Xenopus eggs have the ability to replicate any kind of DNA, AT-rich domains or transcription factors favor the selection of DNA replication origins without increasing the overall efficiency of DNA synthesis. These results suggest that asymmetric AT-rich regions might be default elements that favor the selection of a DNA replication origin in a transcriptionally silent complex, whereas other epigenetic elements linked to the organization of domains for transcription may have further evolved over this basal layer of regulation.
Stanojcic
Slavica
S
Institute of Human Genetics, CNRS, 141 Rue de la Cardonille, 34396 Montpellier Cedex 5, France.
Lemaitre
Jean-Marc
JM
Brodolin
Konstantin
K
Danis
Etienne
E
Mechali
Marcel
M
D020856:AT Rich Sequence / Q000235:genetics*; D000818:Animals; D020029:Base Pairing; D001483:Base Sequence; D002457:Cell Extracts; D002843:Chromatin / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004261:DNA Replication; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D008969:Molecular Sequence Data; D051738:Origin Recognition Complex / Q000378:metabolism; D010063:Ovum / Q000378:metabolism*; D011485:Protein Binding; D013094:Spermatozoa / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014981:Xenopus / Q000235:genetics*
D002457:Cell Extracts; D002843:Chromatin; D051738:Origin Recognition Complex; D014157:Transcription Factors; D004247:DNA