Effect of addition of Tween 80 and potassium dihydrogenphosphate to basal medium on the isolation of marine eukaryotes, thraustochytrids.
Tween 80, KH(2)PO(4) and tomato juice were added to basal medium for the isolation of thraustochytrids. By the addition of Tween 80 and KH(2)PO(4), the number of thraustochytrids isolated from seawater increased. KH(2)PO(4) and Tween 80 were considered to be useful for isolating thraustochytrids.
Department of Biological Production and Environmental Science, Laboratory of Marine Bioscience, Faculty of Agriculture, Miyazaki University, Miyazaki, Japan.
Yousuke
Y
Taoka
Naoki
N
Nagano
Yuji
Y
Okita
Hitoshi
H
Izumida
Shinichi
S
Sugimoto
Masahiro
M
Hayashi
D002469:Cell Separation / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005057:Eukaryotic Cells / Q000166:cytology*; D010710:Phosphates / Q000737:chemistry*; D011136:Polysorbates / Q000737:chemistry*; D017680:Potassium Compounds / Q000737:chemistry*
D003470:Culture Media; D010710:Phosphates; D011136:Polysorbates; D017680:Potassium Compounds; C013216:potassium phosphate