Genetic contribution of the CD14 -159C/T dimorphism in the promoter region in Japanese RA.
OBJECTIVE
To study the contribution of the CD14 gene to the pathogenesis of rheumatoid arthritis (RA) in Japanese patients.


METHODS
CD14 genotyping was carried out at the -159C/T dimorphic site in 97 RA patients and 104 normal subjects by the PCR-RFLP (restriction fragment length polymorphism)


METHODS
HLA-DRB1 genotyping was performed by the PCR-SSCP (sequence specific conformational polymorphism) method.


RESULTS
The -159C/T dimorphism is not associated with whole RA or with female RA, and the results were compatible with a previous report from Germany. The -159C/T dimorphism was not associated with rheumatoid factor (RF)-positive RA, although the -159T allele tended to be associated with RF in the German report. The -159C/T dimorphism showed no association even in RA patients with the RA-susceptibility HLA-DRB1*0405. The -159T allele was prevalent in Japanese controls.


CONCLUSIONS
The CD14 gene is very unlikely to be genetically involved in the pathogenesis of Japanese RA.
Department of Internal Medicine (Allergy and Rheumatology), Faculty of Medicine, University of Tokyo, Tokyo, Japan. fukio-tky@umin.ac.jp
F
F
Takeuchi
N
N
Nakaue
N
N
Kobayashi
S
S
Kuwata
T
T
Murayama
K
K
Kawasugi
M
M
Mori
K
K
Matsuta
D000368:Aged; D001172:Arthritis, Rheumatoid / Q000208:ethnology* / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000208:ethnology; D005838:Genotype; D006801:Humans; D007564:Japan / Q000453:epidemiology; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length; D018807:Polymorphism, Single-Stranded Conformational; D015995:Prevalence; D011401:Promoter Regions, Genetic / Q000235:genetics
D018950:Lipopolysaccharide Receptors