Interleukin-1 beta promotes sensory nerve regeneration after sciatic nerve injury.
Nerve injury brings about axonal disconnection, and thus axonal extension is one of the important steps for nerve regeneration. Expression of the pro-inflammatory cytokine interleukin-1 beta (IL-1beta) is increased at the early stage of nervous system injury, and previously IL-1beta has been reported to promote neurite outgrowth by inhibiting RhoA activity in vitro. However, the effect of IL-1beta on axonal extension in vivo has not been obvious. Now we examine whether IL-1beta takes advantages on sciatic nerve regeneration. Sciatic nerves of rats are transected and sutured, and IL-1beta or PBS is locally administered for 2 weeks. Although IL-1beta does not influence on motor functional recovery, it promotes sensory functional recovery, estimated by toe pinch test, and increases the number and the area of neurofilament-positive axons at 12 weeks compared with PBS. Moreover IL-1beta, which promotes Schwann cell proliferation and thus may inhibit myelination, does not impair remyelination, estimated by myelin basic protein. These findings suggest that IL-1beta may contribute to sensory nerve regeneration following sciatic nerve injury by promoting axonal extension.
Department of Orthopaedics, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Ko
K
Temporin
Hiroyuki
H
Tanaka
Yusuke
Y
Kuroda
Kiyoshi
K
Okada
Koji
K
Yachi
Hisao
H
Moritomo
Tsuyoshi
T
Murase
Hideki
H
Yoshikawa
D000818:Animals; D001369:Axons / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D005260:Female; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000008:administration & dosage / Q000494:pharmacology*; D008856:Microscopy, Fluorescence; D009043:Motor Activity / Q000187:drug effects / Q000502:physiology; D009186:Myelin Sheath / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D009416:Nerve Regeneration / Q000187:drug effects* / Q000502:physiology; D016900:Neurofilament Proteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects / Q000502:physiology; D009475:Neurons, Afferent / Q000187:drug effects / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects*; D012583:Schwann Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D012584:Sciatic Nerve / Q000293:injuries / Q000502:physiology*; D013997:Time Factors
D053583:Interleukin-1beta; D016900:Neurofilament Proteins