Determination of Fumonisin B1 in animal tissues with immunoaffinity purification.
Immunoaffinity extraction combined with high-performance liquid chromatography (HPLC) with fluorescence detection was developed to determine Fumonisin B1 (FB1) in duck tissues. The method was linear over a concentration range of 0.013-0.250 microg of FB1/g of liver, kidney and muscle. The limit of quantification was 0.013 microg FB1/g in tissue. The mean percentage of extraction was 75% for liver and kidney and 53% for muscle. This method can be used in duck for the detection of FB1 contamination after exposure, the liver being the most contaminated tissue.
Tardieu
Didier
D
Unité de Mycotoxicologie, Ecole Nationale Vétérinaire de Toulouse, 23 chemin des Capelles, B.P. 87614, 31076 Toulouse cedex, France. d.tardieu@envt.fr
Auby
Aliénor
A
Bluteau
Caroline
C
Bailly
Jean Denis
JD
Guerre
Philippe
P
D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004372:Ducks; D037341:Fumonisins / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*
D002457:Cell Extracts; D037341:Fumonisins; C056933:fumonisin B1