Enantioseparation of omeprazole and its metabolite 5-hydroxyomeprazole using open tubular capillary electrochromatography with immobilized avidin as chiral selector.
The present paper demonstrates the enantiomeric separation of omeprazole and its metabolite 5-hydroxyomeprazole performed with open tubular capillary electrochromatography (OT-CEC). The protein avidin was used as the chiral selector. Avidin was immobilized by a Schiffs base type of reaction where the protein was via glutaraldehyde covalently bonded to the amino-modified wall of a fused-silica capillary, 50 microm i.d. Both racemates were baseline resolved. Resolution was 1.9 and 2.3, respectively, using ammonium acetate buffer, pH 5.8, 5% methanol, with UV-detection. These values of resolution using OT-CEC are higher than earlier published results regarding chiral separation of omeprazole and 5-hydroxyomeprazole on packed CEC. The number of theoretical plates also indicated good separation efficiency.
Department of Chemistry and Biomedical Sciences, Karlstad University, Universitetsgatan 2, SE-651 88 Karlstad, Sweden.
J
J
Olsson
L G
LG
Blomberg
D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000302:isolation & purification*; D001360:Avidin / Q000737:chemistry; D053801:Capillary Electrochromatography / Q000295:instrumentation* / Q000379:methods; D009853:Omeprazole / Q000302:isolation & purification*; D015203:Reproducibility of Results; D013237:Stereoisomerism
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D001360:Avidin; C049963:5-hydroxymethylomeprazole; D009853:Omeprazole