Transgenesis via permanent integration of genes in repopulating spermatogonial cells in vivo.
Current techniques for making transgenic mice are cumbersome, requiring trained personnel, costly infrastructure and collection of many zygotes from mice that are then killed. We developed a reproducible nonterminal technique for transfecting genes in undifferentiated spermatogonia through in vivo electroporation of the testis; about 94% of male mice electroporated with different transgenes successfully sired transgenic pups. Such electroporated males provide a valuable resource for continuous production of transgenic founders for more than a year.
Division of Embryo Biotechnology, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi 110 067, India.
Suveera
S
Dhup
Subeer S
SS
Majumdar
D000818:Animals; D002454:Cell Differentiation; D018274:Electroporation; D005260:Female; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics; D008297:Male; D051379:Mice; D008822:Mice, Transgenic / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics; D013093:Spermatogonia / Q000166:cytology* / Q000378:metabolism; D013737:Testis / Q000166:cytology / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D012333:RNA, Messenger; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins