Inhibitory effects of triphlorethol-A on MMP-1 induced by oxidative stress in human keratinocytes via ERK and AP-1 inhibition.
Oxidative stress is known to generate reactive oxygen species (ROS) in cells, which subsequently induce the synthesis of matrix metalloproteinases (MMP) and an aging phenomenon. The protective effects of triphlorethol-A, derived from Ecklonia cava, were investigated against hydrogen peroxide (H(2)O(2))-induced damage using human skin keratinocytes. Data showed that triphlorethol-A inhibited ROS formation, induced catalase expression, inhibited DNA damage, and increased cell viability in keratinocytes. Triphlorethol-A treatment significantly reduced MMP-1 expression and production, compared to H(2)O(2)-treated cells. In addition, triphlorethol-A abrogated the activation of extracellular signal regulated protein kinase (ERK), which originates upstream of MMP-1 expression, and was induced by H(2)O(2) treatment. Moreover, triphlorethol-A inhibited DNA binding activity of activator protein-1 (AP-1), a downstream transcription factor of ERK. Data indicate that the antioxidative properties of triphlorethol-A involve the inhibition of MMP-1 via ERK and AP-1 inhibition.
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si,Korea.
Kyoung Ah
KA
Kang
Rui
R
Zhang
Mei Jing
MJ
Piao
Dong Ok
DO
Ko
Zhi Hong
ZH
Wang
Kyung
K
Lee
Bum Joon
BJ
Kim
Taekyun
T
Shin
Jae Woo
JW
Park
Nam Ho
NH
Lee
Byoung Sam
BS
Yoo
Jin Won
JW
Hyun
D015153:Blotting, Western; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D020552:Comet Assay; D004247:DNA / Q000187:drug effects; D004249:DNA Damage; D004790:Enzyme Induction; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors*; D019084:Fluorescent Antibody Technique, Indirect; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015603:Keratinocytes / Q000187:drug effects* / Q000201:enzymology; D020781:Matrix Metalloproteinase 1; D016877:Oxidants / Q000494:pharmacology; D018384:Oxidative Stress; D010696:Phloroglucinol / Q000031:analogs & derivatives* / Q000494:pharmacology; D018808:Transcription Factor AP-1 / Q000037:antagonists & inhibitors*
D016166:Free Radical Scavengers; D016877:Oxidants; D018808:Transcription Factor AP-1; C504400:triphlorethol-A; D004247:DNA; D006861:Hydrogen Peroxide; D010696:Phloroglucinol; D002374:Catalase; D048049:Extracellular Signal-Regulated MAP Kinases; D020781:Matrix Metalloproteinase 1