Changes in the initial phase of lipid peroxidation induced by elicitor from Phytophthora infestans in Solanum species.
The initial phase of the lipid peroxidation process in leaves of Solanum nigrum var. gigantea, Solanum tuberosum cv Bzura and clone H-8105, which represent non-host resistance, field resistance and susceptibility, respectively, against Phytophthora infestans, was investigated. Based on quantitative and qualitative high-performance liquid chromatography (HPLC) analyses of free and esterified fatty acid hydroperoxides (FAHs), we characterized the lipid peroxidation process induced by the pathogen-derived elicitor, culture filtrate (CF), in leaves of the studied genotypes. In all plants, FAHs generated due to 13-lipoxygenase (LOX) action dominated over those from the non-enzymatic pathway. The FAHs derived from 9-LOX activity were found only in CF-treated leaves of the non-host resistant S. nigrum. However, experiments in vitro and in planta with exogenous linoleic acid (LA) as a substrate for LOX revealed high constitutive activity of 9-LOX in all genotypes, which increased in response to CF treatment. The time course changes in polyunsaturated fatty acid (PUFA) pools in the total lipid fractions as well as the degree of their oxidation suggested that CF-induced PUFA peroxidation was enhanced mostly in S. nigrum, less so in Bzura and least in the susceptible clone H-8105. The obtained results are discussed in light of the overall biochemical cell status of plants in the studied interactions.
Polkowska-Kowalczyk
Lidia
L
Department of Plant Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 Warsaw, Poland.
Montillet
Jean-Luc
JL
Agnel
Jean-Pierre
JP
Triantaphylidès
Christian
C
Wielgat
Bernard
B
Maciejewska
Urszula
U
D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry; D015227:Lipid Peroxidation; D008084:Lipoxygenase / Q000378:metabolism; D010084:Oxidation-Reduction; D055750:Phytophthora infestans / Q000502:physiology*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology / Q000382:microbiology; D012987:Soil; D032322:Solanum / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism* / Q000382:microbiology*; D013045:Species Specificity; D013237:Stereoisomerism; D013379:Substrate Specificity; D013997:Time Factors
D005227:Fatty Acids; D010936:Plant Extracts; D012987:Soil; D008084:Lipoxygenase