[Genetic imprinted gene PEG10 expression in deciduas from inevitable abortion].
The expression and distribution of the imprinted paternally expressed gene 10 (PEG10) in decidual tissues of 36 patients with inevitable abortion and 36 normal pregnant (NP) women were examined by RT-PCR, immunohistochemistry, in situ hybridization and Western blot. Decidual PEG10 mRNA expression was significantly lower in the patients than that in the NP group by RT-PCR (P<0.05). The characteristic PEG10 expression was confirmed by in situ hybridization. As shown by Western blot and immunohistochemistry, PEG10 protein expression was consistent with the mRNA expression pattern. The results showed that maintaining sufficient levels of PEG10 may be important during early pregnancy. Lower levels of PEG10 might be one of reasons that underlie inevitable abortion.
Liang
Xiao-Yan
XY
Laboratory of Reproductive Biology, Department of Genetics, School of Public Health, Chongqing Medical University, Chongqing 400016, China. binjilingyan@163.com
Liu
Xue-Qing
XQ
Ding
Yu-Bin
YB
Chen
Xue-Mei
XM
Wang
Ying-Xiong
YX
D000022:Abortion, Spontaneous / Q000235:genetics* / Q000378:metabolism*; D000328:Adult; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D004268:DNA-Binding Proteins; D003656:Decidua / Q000378:metabolism*; D005260:Female; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008297:Male; D011247:Pregnancy; D011506:Proteins / Q000235:genetics*; D016601:RNA-Binding Proteins; D020133:Reverse Transcriptase Polymerase Chain Reaction; D055815:Young Adult
D051017:Apoptosis Regulatory Proteins; D004268:DNA-Binding Proteins; C432965:PEG10 protein, human; D011506:Proteins; D016601:RNA-Binding Proteins