Multiclass pesticide determination in olives and their processing factors in olive oil: comparison of different olive oil extraction systems.
The processing factors (pesticide concentration found in olive oil/pesticide concentration found in olives) of azinphos methyl, chlorpyrifos, lambda-cyhalothrin, deltamethrin, diazinon, dimethoate, endosulfan, and fenthion were determined in olive oil production process in various laboratory-scale olive oil extractions based on three- or two-phase centrifugation systems in comparison with samples collected during olive oil extractions in conventional olive mills located at different olive oil production areas in Greece. Pesticide analyses were performed using a multiresidue method developed in our laboratory for the determination of different insecticides and herbicides in olive oil by solid-phase extraction techniques coupled to gas chromatography detection (electron capture detection and nitrogen phosphorus detection), optimized, and validated for olive fruits sample preparation. Processing factors were found to vary among the different pesticides studied. Water addition in the oil extraction procedure (as in a three-phase centrifugation system) was found to decrease the processing factors of dimethoate, alpha-endosulfan, diazinon, and chlorpyrifos, whereas those of fenthion, azinphos methyl, beta-endosulfan, lambda-cyhalothrin, and deltamethrin residues were not affected. The water content of olives processed was found to proportionally affect pesticide processing factors. Fenthion sulfoxide and endosulfan sulfate were the major metabolites of fenthion and endosulfan, respectively, that were detected in laboratory-produced olive oils, but only the concentration of fenthion sulfoxide was found to increase with the increase of water addition in the olive oil extraction process.
Laboratory of Industrial Chemistry, Research Unit of Environmental Chemistry and Technology, Department of Chemistry, University of Ioannina, Ioannina 45110, Greece. me00753@cc.uoi.gr
Elpiniki G
EG
Amvrazi
Triantafyllos A
TA
Albanis
D002849:Chromatography, Gas; D005511:Food Handling / Q000379:methods; D005638:Fruit / Q000737:chemistry*; D006540:Herbicides / Q000032:analysis; D007306:Insecticides / Q000032:analysis; D031658:Olea / Q000737:chemistry*; D000069463:Olive Oil; D010575:Pesticides / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry* / Q000302:isolation & purification*; D014867:Water / Q000032:analysis
D006540:Herbicides; D007306:Insecticides; D000069463:Olive Oil; D010575:Pesticides; D010938:Plant Oils; D014867:Water