Hepatitis C virus prevalence and genetic diversity among pregnant women in Gabon, central Africa.
BACKGROUND
Hepatitis C virus (HCV) infection is a major global public health problem in both developed and developing countries. The prevalence and genetic diversity of HCV in pregnant women in Gabon, central Africa, is not known. We therefore evaluated the prevalence and the circulating genotypes of HCV in a large population cohort of pregnant women.


METHODS
Blood samples (947) were collected from pregnant women in the five main cities of the country. The prevalence was evaluated by two ELISA tests, and the circulating genotypes were characterized by sequencing and phylogenetic analysis.


RESULTS
Twenty pregnant women (2.1%) were infected with HCV. The seroprevalence differed significantly by region (p = 0.004) and increased significantly with age (p = 0.05), being 1.3% at 14-20 years, 1.1% at 21-25 years, 1.9% at 26-30 years, 4.1% at 31-35 years and 6.0% at > 35 years. Sequencing in the 5'-UTR and NS5B regions showed that the circulating strains belonged to genotypes 4 (4e and 4c).


CONCLUSIONS
We found that the HCV seroprevalence in pregnant women in Gabon is almost as high as that in other African countries and increases with age. Furthermore, only genotype 4 (4e and 4c) was found. More extensive studies aiming to evaluate the prevalence and heterogeneity of HCV genotypes circulating in the general population of the country are needed.
Ndong-Atome
Guy-Roger
GR
Laboratoire de Virologie, Centre International de Recherches Médicales (CIRMF), BP 769, Franceville, Gabon. roger_ndong@yahoo.com
Makuwa
Maria
M
Njouom
Richard
R
Branger
Michel
M
Brun-Vézinet
Francoise
F
Mahé
Antoine
A
Rousset
Dominique
D
Kazanji
Mirdad
M
D000293:Adolescent; D000328:Adult; D017677:Age Distribution; D030561:Databases, Nucleic Acid; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005681:Gabon / Q000453:epidemiology; D014644:Genetic Variation; D005838:Genotype; D005843:Geography; D016174:Hepacivirus / Q000235:genetics* / Q000302:isolation & purification; D006526:Hepatitis C / Q000097:blood / Q000453:epidemiology* / Q000821:virology; D006801:Humans; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000097:blood / Q000453:epidemiology* / Q000821:virology; D015995:Prevalence; D012367:RNA, Viral / Q000097:blood
D012367:RNA, Viral