Lipoperoxidation and protein oxidative damage exhibit different kinetics during septic shock.
Septic shock (SS)-related multiorgan dysfunction has been associated with oxidative damage, but little is known about the temporal damage profile and its relationship to severity. The present work investigated prospectively 21 SS patients. Blood samples were obtained at diagnosis, 24, 72 hours, day 7, and at 3 months. At admission, thiobarbituric acid reactive substances (TBARSs), plasma protein carbonyls, plasma protein methionine sulfoxide (MS), ferric/reducing antioxidant power (FRAP), total red blood cell glutathione (RBCG), uric acid (UA), and bilirrubin levels were increased (P < .05). Total radical-trapping antioxidant potential (TRAP) and vitamin-E were similar to controls, and vitamin-C was decreased (P < .05). During evolution, TBARS and RBCG increased (P < .001), vitamin-E levels remained stable, whereas plasma protein carbonyls and MS, TRAP, vitamin-C, reduced glutathione, and UA levels decreased (P < .006). After 3 months, plasma protein carbonyls and MS persisted elevated. More severe patients exhibited higher TBARS, TRAP, FRAP, vitamin-C, UA, and bilirrubin levels. Our results suggest early and persistent oxidative stress during septic shock and a correlation between increasing levels of lipoperoxidation and sepsis severity.
Departamento de Medicina Intensiva, Facultad de Medicina, Pontificia Universidad Católica de Chile, Alameda, 340 Santiago, Chile.
Max
M
Andresen
Tomas
T
Regueira
Alejandro
A
Bruhn
Druso
D
Perez
Pablo
P
Strobel
Alberto
A
Dougnac
Guillermo
G
Marshall
Federico
F
Leighton
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000975:Antioxidants / Q000378:metabolism; D001205:Ascorbic Acid / Q000097:blood; D001663:Bilirubin / Q000097:blood; D001798:Blood Proteins / Q000737:chemistry / Q000378:metabolism*; D005260:Female; D005978:Glutathione / Q000097:blood; D006801:Humans; D007700:Kinetics; D015227:Lipid Peroxidation; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D011446:Prospective Studies; D050050:Protein Carbonylation; D012772:Shock, Septic / Q000097:blood* / Q000473:pathology; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D013997:Time Factors; D014810:Vitamin E / Q000097:blood
D000975:Antioxidants; D001798:Blood Proteins; D017392:Thiobarbituric Acid Reactive Substances; D014810:Vitamin E; D005978:Glutathione; D001205:Ascorbic Acid; D001663:Bilirubin