Arsenic enhances matrix metalloproteinase-14 expression in fibroblasts.
This study focused on the effects of arsenic (As) on fibroblast-derived matrix metalloproteinase (MMP)-2 and -14 levels, as these proteins were reported to be associated with tumor progression. Arsenic was found to promote production of the fibroblast-derived active form of MMP-2. Further, As at 100 or 1000 microM increased MMP-14 expression levels in fibroblasts. In addition, 1000 microM mercury (Hg) but not As increased pro-MMP-2 protein, which is involved in the conversion of the proenzyme into its active form. Since MMP-14 is an activator of pro-MMP-2, data suggest that As promotes production of fibroblast-derived active form of MMP-2 through increased expression of MMP-14. Evidence indicates that As appeared to be less effective than Hg in the conversion of pro-MMP-2 into its active form.
Unit of Environmental Health Sciences, Department of Biomedical Sciences, College of Life and Health Sciences, Chubu University, Aichi, Japan. katomasa@isc.chubu.ac.jp
Masashi
M
Kato
Khaled
K
Hossain
Machiko
M
Iida
Hiroshi
H
Sato
Noriyuki
N
Uemura
Yuji
Y
Goto
D001151:Arsenic / Q000494:pharmacology*; D002478:Cells, Cultured; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000201:enzymology / Q000378:metabolism*; D053511:Matrix Metalloproteinase 14 / Q000187:drug effects* / Q000378:metabolism; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects* / Q000378:metabolism
D020778:Matrix Metalloproteinase 2; D053511:Matrix Metalloproteinase 14; D001151:Arsenic