Identification of pongamol and karanjin as lead compounds with antihyperglycemic activity from Pongamia pinnata fruits.
OBJECTIVE
To identify pongamol and karanjin as lead compounds with antihyperglycemic activity from Pongamia pinnata fruits.


METHODS
Streptozotocin-induced diabetic rats and hyperglycemic, hyperlipidemic and hyperinsulinemic db/db mice were used to investigate the antihyperglycemic activity of pongamol and karangin isolated from the fruits of Pongamia pinnata.


RESULTS
In streptozotocin-induced diabetic rats, single dose treatment of pongamol and karanjin lowered the blood glucose level by 12.8% (p<0.05) and 11.7% (p<0.05) at 50mg /kg dose and 22.0% (p<0.01) and 20.7% (p<0.01) at 100mg/kg dose, respectively after 6h post-oral administration. The compounds also significantly lowered blood glucose level in db/db mice with percent activity of 35.7 (p<0.01) and 30.6 (p<0.01), respectively at 100mg/kg dose after consecutive treatment for 10 days. The compounds were observed to exert a significant inhibitory effect on enzyme protein tyrosine phosphatase-1B (EC 3.1.3.48).


CONCLUSIONS
The results showed that pongamol and karangin isolated from the fruits of Pongamia pinnata possesses significant antihyperglycemic activity in Streptozotocin-induced diabetic rats and type 2 diabetic db/db mice and protein tyrosine phosphatase-1B may be the possible target for their activity.
Division of Biochemistry, Central Drug Research Institute, Lucknow, India.
Akhilesh K
AK
Tamrakar
Prem P
PP
Yadav
Priti
P
Tiwari
Rakesh
R
Maurya
Arvind K
AK
Srivastava
D041721:3T3-L1 Cells; D000818:Animals; D001572:Benzofurans / Q000494:pharmacology*; D001578:Benzopyrans / Q000494:pharmacology*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D005638:Fruit / Q000737:chemistry*; D007004:Hypoglycemic Agents / Q000494:pharmacology*; D008297:Male; D051379:Mice; D033641:Millettia / Q000737:chemistry*; D010749:Phosphoprotein Phosphatases / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000032:analysis; D000071636:Protein Phosphatase 2C; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin
D001572:Benzofurans; D001578:Benzopyrans; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D013311:Streptozocin; C533419:pongamol; D010749:Phosphoprotein Phosphatases; D000071636:Protein Phosphatase 2C; C052068:karanjin