[Longbixiao capsule reduces expressions of TGF-beta1 and Smoothelin in human prostatic stromal cells cultured in vitro].
OBJECTIVE
To investigate the effects of the Chinese herbal medicine of Longbixiao (LBX) Capsule on the expressions of TGF-beta1 and Smoothelin in human prostatic stromal cells cultured in vitro.


METHODS
Blood serum medicated with LBX was incubated with the stromal cells isolated from men with benign prostatic hyperplasia (BPH) and cultured in vitro. The mRNA expression levels of TGF-beta1 and Smoothelin were detected by real-time RT-PCR and other relevant techniques.


RESULTS
In the high and low concentration groups, the gene relative expressions of TGF-beta1 were (0.158 +/- 0.020) and (0.169 +/- 0.020) , while those of Smoothelin were (0.035 +/- 0.007) and (0.036 +/- 0.007) respectively, both significantly decreased in comparison with the control group(P < 0.01).


CONCLUSIONS
LBX reduces the mRNA expressions of TGF-beta1 and Smoothelin in human prostatic stromal cells and can be used in the treatment of BPH.
Department of Andrology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China. wgma66@tom.com
Wei-Guo
WG
Ma
Jin-Ming
JM
Jia
Yong-Zheng
YZ
Jiao
Jia-Hui
JH
Wang
Shao-Bo
SB
Luo
Ju
J
Zhang
Jian-Dang
JD
Shi
Zhi-Song
ZS
Zhang
D000818:Animals; D002214:Capsules; D002478:Cells, Cultured; D003598:Cytoskeletal Proteins / Q000235:genetics*; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D006801:Humans; D008297:Male; D009124:Muscle Proteins / Q000235:genetics*; D011470:Prostatic Hyperplasia / Q000473:pathology; D011817:Rabbits; D020133:Reverse Transcriptase Polymerase Chain Reaction; D044967:Serum / Q000737:chemistry; D017154:Stromal Cells / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D053773:Transforming Growth Factor beta1 / Q000235:genetics*
D002214:Capsules; D003598:Cytoskeletal Proteins; D004365:Drugs, Chinese Herbal; D009124:Muscle Proteins; C100762:SMTN protein, human; D053773:Transforming Growth Factor beta1; C539082:longbixiao