Protective effect of the aqueous leaf and seed extract of Phyllanthus amarus on gentamicin and acetaminophen-induced nephrotoxic rats.
OBJECTIVE
In African traditional medicine, different parts of Phyllanthus amarus Schum. and Thonn. (family: Euphorbiaceae) are highly valued for the treatment of array of human diseases including hepatic and urolithic and/or other renal diseases. In the present study, single oral 100-400mg/kg/day of the leaf and seed aqueous extract of Phyllanthus amarus (PA) were studied for their protective effects in acetaminophen- and gentamicin-induced nephrotoxic Wistar rats for 14 days.


METHODS
In each model of nephrotoxicities, thirty adult male Wistar rats were evenly divided into 5 groups. Groups I and II served as untreated and model controls, respectively while groups III-V were the treatment groups which were pretreated with 100-400mg/kg/day of PA 1hr before each dose of the nephrotoxicants for 14 days. On the 15th day, blood samples for serum urea and creatinine while the rat kidneys for histology were obtained under inhaled diethyl ether anesthesia.


RESULTS
In the acetaminophen nephrotoxic rats, 100-400mg/kg/day significantly (p<0.05, p<0.01, p<0.001) attenuated elevations in the serum creatinine and blood urea nitrogen levels in dose related fashion, as well as, attenuation of acetaminophen-induced tubulonephrosis. Similar effects were also recorded in the gentamicin model of acute renal injury. Results suggest that the nephroprotective effect of PA could be due to the inherent antioxidant and free-radical-scavanging principle(s) contained in the extract.


CONCLUSIONS
In the near future, PA could constitute a lead to discovery of a novel drug for the treatment of drug-induced nephrotoxicity.
Department of Pharmacology, Faculty of Basic Medical Sciences, Lagos State University College of Medicine, P.M.B. 21266, Ikeja, Lagos State, Nigeria. adeneye2001@yahoo.com
Adejuwon Adewale
AA
Adeneye
Adokiye Senebo
AS
Benebo
D000082:Acetaminophen / Q000633:toxicity; D000818:Animals; D001806:Blood Urea Nitrogen; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology / Q000517:prevention & control*; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D016166:Free Radical Scavengers / Q000008:administration & dosage / Q000302:isolation & purification / Q000494:pharmacology; D005839:Gentamicins / Q000633:toxicity; D008099:Liver / Q000187:drug effects* / Q000473:pathology; D008297:Male; D029803:Phyllanthus / Q000737:chemistry*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D018515:Plant Leaves; D051381:Rats; D017208:Rats, Wistar; D012639:Seeds
D016166:Free Radical Scavengers; D005839:Gentamicins; D010936:Plant Extracts; D000082:Acetaminophen; D003404:Creatinine