Rapid and accurate G M1-gangliosidosis diagnosis using a parentage testing microsatellite.
The G M1-gangliosidosis is an autosomal recessive lysosomal storage disease caused by structural defects of the beta-galactosidase gene (GLB1) which lead to a severe phenotypical impairment in homozygous individuals, whereas heterozygous carriers remain clinically normal. Currently employed DNA parentage tests include the analysis of microsatellites, which also have a diagnostic predictive value. The aim of this study was to provide a reliable tool for genotyping the canine GLB1 which can be effectively integrated in parentage testing investigations. For this purpose the association between the GLB1 gene and the AHT K253 microsatellite was analyzed in 30 Alaskan huskies (11 GLB1+/+, 17 GLB1+/- and 2 GLB1-/- dogs). The 143 bp AHT K253 microsatellite allele was identified only in GLB1+/- and GLB1-/- animals and was in strong linkage disequilibrium with the causative mutation for G M1-gangliosidosis, a 19 bp duplication within exon 15 of the GLB1 gene. The results of the present study revealed a 100% concordance between the previous established genotypes and those obtained after the analysis of the AHT K253 microsatellite. Thus, the genotype of the AHT K253 microsatellite, which is routinely determined during dog parentage testing, has a high predictive value for the G M1-gangliosidosis carrier status.
Kreutzer
Robert
R
Department of Pathology, University of Veterinary Medicine, Bünteweg 17, Hannover, Germany.
Kreutzer
Mihaela
M
Leeb
Tosso
T
Baumgärtner
Wolfgang
W
D000483:Alleles; D000818:Animals; D004285:Dogs; D005260:Female; D016537:Gangliosidosis, GM1 / Q000175:diagnosis* / Q000235:genetics*; D008297:Male; D018895:Microsatellite Repeats / Q000235:genetics*; D017422:Sequence Analysis, DNA / Q000379:methods*; D013997:Time Factors