Generation and validation of affinity reagents on a proteome-wide level.
There is a need for protein-specific affinity reagents to explore the gene products encoded by the genome. Recently, systematic efforts to generate validated affinity reagents on a whole human proteome level have been initiated. There are several issues for such efforts, including choice of antigen, type of affinity reagent, and the subsequent validation of the generated protein-specific binders. The advantages and disadvantages with the different approaches are discussed and the problems related to quality assessment of antibodies to be used in multi-platform applications are addressed. This review also describes the efforts to create a virtual resource of validated antibodies using a community-based portal and summarizes the status and visions for the publicly available human protein atlas (http://www.proteinatlas.org) showing the human protein profiles in a large number of normal and cancer tissues as well as a large set of human cell lines.
Uhlén
Mathias
M
Department of Proteomics, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Center, Stockholm, Sweden. mathias@biotech.kth.se
Hober
Sophia
S
D000345:Affinity Labels; D006801:Humans; D011506:Proteins / Q000276:immunology* / Q000378:metabolism*; D020543:Proteome / Q000276:immunology* / Q000378:metabolism*
D000345:Affinity Labels; D011506:Proteins; D020543:Proteome