Cell-to-cell communication: time and length scales of ligand internalization in cultures of suspended cells.
A problem of cell-to-cell communication by diffusible ligands is analyzed for the case when cells are distributed in three dimensions and diffusible ligands are secreted by cells and reversibly bind to cell surface receptors. Following its binding to a receptor, the ligand can either dissociate and be released back in the medium or be absorbed by the cell in a process that is called internalization. Using an effective medium approximation, we derive analytical expressions that characterize the time and length scales associated with the ligand trajectories leading to internalization. We discuss the applicability of our approximation and illustrate the application of our results to a specific cellular system.
Mathematical and Statistical Computing Laboratory, Division of Computational Bioscience, Center for Information Technology, National Institutes of Health, Bethesda, Maryland 20892, USA.
Alexander M
AM
Berezhkovskii
Mathieu
M
Coppey
Stuart C
SC
Sealfon
Stanislav
S
Shvartsman
D002450:Cell Communication; D002478:Cells, Cultured; D003470:Culture Media; D004058:Diffusion; D004705:Endocytosis; D008024:Ligands; D008954:Models, Biological; D011956:Receptors, Cell Surface / Q000378:metabolism; D013997:Time Factors
D003470:Culture Media; D008024:Ligands; D011956:Receptors, Cell Surface