Humoral antibody response of the tilapia Oreochromis niloticus against Cichlidogyrus spp. (Monogenea).
The humoral immune response of the tilapia Oreochromis niloticus was evaluated using a direct ELISA. Serum was tested from fish infected with Cichlidogyrus spp. (Monogenea) and from fish injected intraperitoneally with the Cichlidogyrus spp. antigenic extract, i.e., 150 microl of the Cichlidogyrus spp. saline extract diluted in Freund's complete adjuvant (FCA) (1:1) were inoculated intraperitoneally at day 0, followed by 2 dosages of 50 microl of the same Cichlidogyrus spp. saline extract diluted in Freund's incomplete adjuvant (FIA) (1:1) at weeks 2 and 4, respectively. The humoral response was also evaluated by the double immunodiffusion test (DID) and by serum protein and total immunoglobulin (Ig) determinations. The IgM OD values in the hyperimmune fish were significantly higher than in the infected and uninfected fish groups. In the DID test, a precipitation (antigen-antibody) band was observed between the Cichlidogyrus spp. saline extract and hyperimmune sera, but not with the other groups. Increases in serum protein concentration and total Igs were observed in the immunized fish at weeks 2 and 10 postinjection. Results from this study suggest that tilapia is capable of producing an induced humoral immune response against an antigenic extract of Cichlidogyrus spp.
Sandoval-Gío
Juan José
JJ
Laboratorio de Inmunología y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN) Unidad Mérida, Carretera Antigua a Progreso Km, 6 AP 73 Cordemex, 97310 Mérida, Yucatán, México.
Rodríguez-Canul
Rossanna
R
Vidal-Martínez
Víctor Manuel
VM
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D001798:Blood Proteins / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005393:Fish Diseases / Q000276:immunology* / Q000469:parasitology; D005779:Immunodiffusion / Q000662:veterinary; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000097:blood; D010986:Platyhelminths / Q000276:immunology*; D017210:Tilapia / Q000469:parasitology*; D014201:Trematode Infections / Q000276:immunology / Q000469:parasitology / Q000662:veterinary*
D000909:Antibodies, Helminth; D001798:Blood Proteins; D007075:Immunoglobulin M