A Synechocystis LexA-orthologue binds direct repeats in target genes.
Although evidence for LexA-orthologues, which do not regulate DNA damage repair, is accumulating, identification of binding sites and regulon members remains poorly characterized. In the cyanobacterium, Synechocystis sp. strain PCC 6803, we have recently identified a LexA-related protein that regulates expression of the crhR RNA helicase gene. Here we show that the Synechocystis LexA-orthologue binds as a dimer to 12 bp direct repeats containing a CTA-N9-CTA sequence conserved in two target genes, lexA and crhR. Characterization of this site provides the basis for identification of additional LexA targets and further evidence for LexA's divergence during evolution.
Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada T6G 2E9.
Laura M
LM
Patterson-Fortin
George W
GW
Owttrim
D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D018983:DNA Footprinting; D004269:DNA, Bacterial / Q000737:chemistry; D019281:Dimerization; D011401:Promoter Regions, Genetic; D020365:RNA Helicases / Q000235:genetics; D012333:RNA, Messenger / Q000378:metabolism; D012091:Repetitive Sequences, Nucleic Acid; D012697:Serine Endopeptidases / Q000378:metabolism*; D046939:Synechocystis / Q000235:genetics*; D000099160:LexA Repressor Protein
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D020365:RNA Helicases; D012333:RNA, Messenger; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein