[Characterization of the cytopathic effect in human bronchial epithelial cell after Human Bocavirus Infection (HBoV)].
OBJECTIVE
In this study, human bronchial epithelial cells were inoculated with positive sputum specimens of HBoV. After four days' infection, cytopathic effects (CPE) were observed by inverted microscopy. These viruses all cause typical cell damages such as rounded and shrivelled, fusion and fallout. These damages got quick following increased future degenerations. The other assay result of CPE within the infected cells were observed by inverted microscopy, have typical "owl's eye" plaque and above 90 percent hemadsorption within the infected cells by erythrocytes for hemadsorption technique. The typical fluorescence lump of nucleus within the infected cells was found by indirect immunofluorescence technique.


CONCLUSIONS
Isolation and identification of HBoV could be done in the human bronchial epithelial cell, and we found some characterizing CPE in the human bronchial epithelial cell after HBoV infection. The above studies pave a way for studying pathogenicity of human bocavirus.
Lin
Feng
F
Wenling Affiliated Hospital, Wenzhou Medical College, Zhejiang, China.
Hou
Jian-yi
JY
Zheng
Min-qiao
MQ
Wu
Feng
F
Zeng
Ai-ping
AP
Li
Hua
H
Zheng
Chang-hua
CH
Chen
Hong
H
Li
Xu-yang
XY
Rao
Gao-feng
GF
Mo
Yi-han
YH
Huang
En-pei
EP
D052660:Bocavirus / Q000502:physiology*; D001980:Bronchi / Q000166:cytology; D016923:Cell Death / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000821:virology*; D019084:Fluorescent Antibody Technique, Indirect; D054884:Host-Pathogen Interactions; D006801:Humans; D008856:Microscopy, Fluorescence