The use of cultured primary bovine colon epithelial cells as a screening model to detect genotoxic effects of heterocyclic aromatic amines in the comet assay.
Isolated epithelial cells from the bovine colon were maintained in dividing monolayer cultures and used as a model system for colon tissue in in vitro toxicological studies. The cytotoxic effects of the heterocyclic aromatic amines 2-amino-3-methylimidazo [4,5-f]quinoline (IQ) and 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhiP) were investigated in these cells and IC(50) values were determined by inhibition of neutral red uptake into the cultured cells. Although PhiP was not cytotoxic up to concentrations of 500 microM, IQ was cytotoxic above 300 microM. The induction of DNA strand breaks in cultured bovine epithelial colon cells was determined using the alkaline single-cell gel electrophoresis (Comet assay) technique, and subsequently, the DNA damage was used as a determinant of genotoxic effects of the heterocyclic aromatic amines in order to establish this system for detection of adverse effects of chemicals in a model system for the colon. In the absence of an external enzymatic metabolizing system (S9 mix) both amines did not induce DNA strand breaks. When S9 mix was used, PhiP induced DNA strand breaks above 10 microM whereas IQ did not show any significant effect at 300 microM. This cell culture system was found to be a useful screening system for testing of compounds that are considered to affect colonic tissue.
Institut fur Arbeitsphysiologie an der Universitat Dortmund, Leibniz Research Centre for Working Environment and Human Factors, Dortmund, Germany.
Wolfram
W
Follmann
Sascha
S
Birkner
D000588:Amines / Q000737:chemistry / Q000633:toxicity*; D000818:Animals; D002273:Carcinogens / Q000737:chemistry / Q000633:toxicity; D002417:Cattle; D018929:Cell Culture Techniques; D003106:Colon / Q000166:cytology*; D020552:Comet Assay; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects*; D006571:Heterocyclic Compounds / Q000737:chemistry / Q000633:toxicity*; D007413:Intestinal Mucosa / Q000166:cytology*
D000588:Amines; D002273:Carcinogens; D006571:Heterocyclic Compounds