Identification of amide protons of glutathione in MR spectra of tumour cells.
Signals attributable to amide protons and used in previous studies to measure intracellular pH were observed in the low-field region of the (1)H-MR spectra of four tumour cell lines: T98G, MCF-7, A172 and HeLa. The signals were more intense in the spectra of the two cell lines (T98G and MCF-7) characterised by higher concentrations of glutathione (GSH). After comparison with (1)H-MR spectra of GSH in solution at different pH values, the peaks were attributed to NHs of the Cys and Gly residues of GSH. Modification of the intracellular concentration of GSH by treatment with buthionine sulfoximine produced comparable decreases in the intensity of aliphatic signals of GSH and NHs under examination. The assignment was therefore confirmed.
Grande
Sveva
S
Dipartimento di Tecnologie e Salute and INFN Gruppo Collegato Sanità, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy.
Luciani
Anna Maria
AM
Rosi
Antonella
A
Guidoni
Laura
L
Viti
Vincenza
V
D000577:Amides / Q000032:analysis* / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis / Q000737:chemistry*; D045744:Cell Line, Tumor; D005978:Glutathione / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009369:Neoplasms / Q000737:chemistry*; D011522:Protons
D000577:Amides; D014408:Biomarkers, Tumor; D011522:Protons; D005978:Glutathione