Vortex configuration flow cell based on low-temperature cofired ceramics as a compact chemiluminescence microsystem.
The integration of optical detection methods in continuous flow microsystems can highly extend their range of application, as long as some negative effects derived from their scaling down can be minimized. Downsizing affects to a greater extent the sensitivity of systems based on absorbance measurements than the sensitivity of those based on emission ones. However, a careful design of the instrumental setup is needed to maintain the analytical features in both cases. In this work, we present the construction and evaluation of a simple miniaturized optical system, which integrates a novel flow cell configuration to carry out chemiluminescence (CL) measurements using a simple photodiode. It consists of a micromixer based on a vortex structure, which has been constructed by means of the low-temperature cofired ceramics (LTCC) technology. This mixer not only efficiently promotes the CL reaction due to the generated high turbulence but also allows the detection to be carried out in the same area, avoiding intensity signal losses. As a demonstration, a flow injection system has been designed and optimized for the detection of cobalt(II) in water samples. It shows a linear response between 2 and 20 microM with a correlation of r > 0.993, a limit of detection of 1.1 microM, a repeatability of RSD = 12.4%, and an analysis time of 17 s. These results demonstrate the suitability of the proposal to the determination of compounds involved in CL reactions by means of an easily constructed versatile device based on low-cost instrumentation.
Ibáñez-García
Núria
N
Sensors and Biosensors Group, Analytical Chemistry Department, Universitat Autonoma de Barcelona, 08193 Bellaterra, Spain.
Puyol
Mar
M
Azevedo
Carlos M
CM
Martínez-Cisneros
Cynthia S
CS
Villuendas
Francisco
F
Gongora-Rubio
M R
MR
Seabra
A C
AC
Alonso
Julián
J