Effect of liquid-polish coating on in vitro biofilm accumulation on provisional restorations: Part 2.
OBJECTIVE
In part 1 of this study, the authors showed that coating polymethyl methacrylate (PMMA) provisional restorations with bonding resin (BR) or liquid polish (LP) significantly reduced early in vivo biofilm formation on these restorations. The aim of the present study was to determine the mechanism through which these materials inhibit biofilm formation.


METHODS
The antimicrobial properties of the tested materials were examined using the agar diffusion test (ADT) and the direct contact test (DCT). Surface energy was determined using contact angle measurements; salivary protein adsorption was analyzed by polyacrylamide gel electrophoresis (SDS-PAGE).


RESULTS
ADT and DCT showed that the tested materials had no antimicrobial properties. Contact angle measurements revealed that liquid polish and PMMA have a similar contact angle, tending toward the hydrophobic region, and that bonding resin was more hydrophilic. SDS-PAGE analysis showed a significant reduction in salivary protein adsorption to the tested materials compared with that to the PMMA control.


CONCLUSIONS
Liquid polish prevents biofilm formation by preventing protein adsorption.
Davidi
Michael Perez
MP
Department of Prosthodontics, Faculty of Dental Medicine, The Hebrew University Hadassah School of Dental Medicine, Jerusalem, Israel. perezdavidi@bezeqint.net
Beyth
Nurit
N
Weiss
Ervin I
EI
Weiss
Ervin I
EI
Eilat
Yuval
Y
Feuerstein
Osnat
O
Sterer
Nir
N
D000179:Acrylates / Q000737:chemistry*; D000327:Adsorption; D001422:Bacterial Adhesion; D018441:Biofilms / Q000254:growth & development*; D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003764:Dental Materials / Q000737:chemistry*; D003773:Dental Plaque / Q000382:microbiology; D003794:Dental Restoration, Temporary; D004591:Electrophoresis, Polyacrylamide Gel; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008422:Materials Testing; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D019279:Resin Cements / Q000737:chemistry*; D012463:Saliva / Q000382:microbiology; D012471:Salivary Proteins and Peptides / Q000737:chemistry; D013295:Streptococcus mutans / Q000502:physiology; D013499:Surface Properties; D017155:Wettability
D000179:Acrylates; C481141:BisCover; D020099:Coated Materials, Biocompatible; D003764:Dental Materials; D019279:Resin Cements; D012471:Salivary Proteins and Peptides; C115612:single bond; D017438:Bisphenol A-Glycidyl Methacrylate; D019904:Polymethyl Methacrylate