[Genotype 1 JEV was isolated again from Liaoning Province, China, 2007].
OBJECTIVE
To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene.


METHODS
Collected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods.


RESULTS
Two new JEV strains, LNDG07-02 and LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8-88% and 97.2%, respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002.


CONCLUSIONS
Genotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning Province.
Meng
Wei-shan
WS
Department of Viral Encephalitis, Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Li
Ming-hua
MH
Wang
Huan-yu
HY
Zhang
Ji-bo
JB
Gao
Yu
Y
Ding
Jun
J
Fu
Shi-hong
SH
Zhai
You-gang
YG
Chen
Zhe
Z
Gou
Jun-qiao
JQ
Zhao
Yu-jun
YJ
Liang
Guo-dong
GD
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000473:pathology / Q000821:virology; D002460:Cell Line; D002681:China / Q000453:epidemiology; D009033:Culicidae / Q000821:virology*; D004664:Encephalitis Virus, Japanese / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D004672:Encephalitis, Japanese / Q000453:epidemiology / Q000821:virology; D005260:Female; D005838:Genotype; D007303:Insect Vectors / Q000821:virology*; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D017422:Sequence Analysis, DNA
D012367:RNA, Viral