Berberine attenuates intestinal disaccharidases in streptozotocin-induced diabetic rats.
Previous studies demonstrated anti-diabetic effects of berberine. However, the facts that berberine had low bioavailability and poor absorption through the gut wall indicated that berberine might exert its antihyperglycaemic effect in the intestinal tract before absorption. The purpose of this study was to investigate whether berberine attenuates disaccharidase activities and beta-glucuronidase activity in the small intestine of streptozotocin (STZ)-induced diabetic rats. Two groups of STZ-induced diabetic rats were treated with protamine zinc insulin (10 U/Kg) subcutaneously twice daily and berberine (100 mg/Kg) orally once daily for 4 weeks, respectively. Both age-matched normal rats and diabetic control rats received physiological saline only. Fasting blood glucose levels, body weight, intestinal disaccharidase and beta-glucuronidase activities in duodenum, jejunum and ileum were assessed for changes. Our findings suggested that berberine treatment significantly decreases the activities of intestinal disaccharidases and beta-glucuronidase in STZ-induced diabetic rats. The results demonstrated that the inhibitory effect on intestinal disaccharidases and beta-glucuronidase of berberine might be one of the mechanisms for berberine as an antihyperglycaemic agent.
Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, China.
Li
L
Liu
Yuanxiong
Y
Deng
Sen
S
Yu
Shousi
S
Lu
Lin
L
Xie
Xiaodong
X
Liu
D000818:Animals; D001599:Berberine / Q000494:pharmacology*; D001647:Bile Acids and Salts / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003627:Data Interpretation, Statistical; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology*; D004186:Disaccharidases / Q000037:antagonists & inhibitors*; D005966:Glucuronidase / Q000378:metabolism; D007202:Indicators and Reagents; D007422:Intestines / Q000187:drug effects / Q000201:enzymology*; D043322:Lactase / Q000378:metabolism; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013393:Sucrase / Q000378:metabolism; D000520:alpha-Glucosidases / Q000378:metabolism
D001647:Bile Acids and Salts; D001786:Blood Glucose; D007202:Indicators and Reagents; D001599:Berberine; D004186:Disaccharidases; D043322:Lactase; D000520:alpha-Glucosidases; D005966:Glucuronidase; D013393:Sucrase