Thionin Thi2.1 from Arabidopsis thaliana expressed in endothelial cells shows antibacterial, antifungal and cytotoxic activity.
Thionins are plant antimicrobial peptides with antibacterial and antifungal activities. Thionin Thi2.1 cDNA from Arabidopsis thaliana was expressed in BVE-E6E7 bovine endothelial cell line and its activity was evaluated against Escherichia coli, Staphylococcus aureus, Candida albicans and different mammal cell lines. Total protein (2.5 microg) from conditioned medium (CM) of clone EC-Thi2.1 inhibited the growth of E. coli, S. aureus (>90%) and C. albicans strains (>80%) in relation to the CM from control cells. Also, CM of EC-Thi2.1 inhibited the viability of several transformed and normal mammal cell lines (38-95%). These results suggest that thionin Thi2.1 is an antimicrobial peptide that could be use in the treatment of mammalian infectious diseases.
Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolás de Hidalgo, Apdo. Postal 53, Administración Chapultepec, Morelia, Michoacan CP 58262, Mexico.
Heber
H
Loeza-Angeles
Eduardo
E
Sagrero-Cisneros
Leticia
L
Lara-Zárate
Erik
E
Villagómez-Gómez
Joel E
JE
López-Meza
Alejandra
A
Ochoa-Zarzosa
D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D015153:Blotting, Western; D002176:Candida albicans / Q000187:drug effects*; D002417:Cattle; D017077:Culture Media, Conditioned; D004727:Endothelium / Q000166:cytology / Q000187:drug effects*; D004926:Escherichia coli / Q000187:drug effects*; D047468:Immunoprecipitation; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000187:drug effects
D023181:Antimicrobial Cationic Peptides; D029681:Arabidopsis Proteins; D017077:Culture Media, Conditioned; D011994:Recombinant Proteins; C097482:THI2.1 protein, Arabidopsis