Prognostic relevance of human papillomavirus L1 capsid protein detection within mild and moderate dysplastic lesions of the cervix uteri in combination with p16 biomarker.
OBJECTIVE
To proof the prognostic relevance of HPV L1 capsid protein detection on colposcopically-guided punch biopsies in combination with p16.


METHODS
Sections of colposcopically-guided punch biopsies from 191 consecutive cases with at least 5 years of follow-up were stained with HPV L1 capsid protein antibodies (Cytoactiv screening antibody) and a monoclonal anti-p16 antibody. Fifty sections were derived from a benign group, 91 from low-grade (cervical intraepithelial neoplasia [CIN 1]) lesions and 50 from high-grade (CIN 2 and 3) lesions.


RESULTS
Overall only 16.1% of the 87 L1-negative, p16-positive CIN lesions showed remission of the lesion compared to 72.4% of the double positive cases. None of the L1/p16 double negative CIN lesions progressed.


CONCLUSIONS
HPV L1 capsid protein detection with Cytoactiv screening antibody seems to be a promising new tool to predict the behavior of HPV-associated (p16-positive) early dysplastic lesions.
Hilfrich
Ralf
R
Cytoimmun Diagnostics, Pirmasens, Germany. hilfrich@cytoimmun.de
Hariri
Jalil
J
D014408:Biomarkers, Tumor / Q000032:analysis*; D036022:Capsid Proteins / Q000032:analysis*; D002584:Cervix Uteri / Q000378:metabolism / Q000473:pathology*; D019941:Cyclin-Dependent Kinase Inhibitor p16; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000032:analysis*; D009856:Oncogene Proteins, Viral / Q000032:analysis*; D011379:Prognosis; D014626:Vaginal Smears; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000473:pathology
D014408:Biomarkers, Tumor; C000614131:CDKN2A protein, human; D036022:Capsid Proteins; D019941:Cyclin-Dependent Kinase Inhibitor p16; C114580:HPV L1 protein, Human papillomavirus; D009363:Neoplasm Proteins; D009856:Oncogene Proteins, Viral