Dynamic changes of matrix metalloproteinase-9 in patients with Klebsiella pneumoniae meningitis.
To quantitate cerebrospinal fluid (CSF) concentrations of matrix metalloproteinase 9 (MMP-9) in adult patients with Klebsiella pneumoniae meningitis and to correlate levels of MMP-9 with parameters of intrathecal inflammation and analyze the kinetic changes of MMP-9. In a prospective cohort study, levels of MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP-1) concentrations were measured in the CSF of six adult patients with meningitis and 11 controls. MMP-9 and TIMP-1 were detected in all of the six patients at presentation and follow up lumbar puncture. CSF levels of MMP-9 (6.71+/-7.29 ng/ml) and TIMP-1(454.3+/-242.9 ng/ml) were higher in patients than in the control group (0.07+/-0.11 ng/ml and 27.14+/-39.34 ng/ml, respectively). Levels of MMP-9 correlated with CSF concentrations of protein, cell count and lactate. Repeated lumbar punctures showed that levels of MMP-9 decrease during clinical recovery, although the levels of MMP-9 in the CSF are variable because of the small number of cases. The relative change in gelatin zymography is comparable to the changes of MMP-9 levels found in ELISA. MMP-9 levels in CSF may be a useful tool in follow-up in patients with K. pneumoniae meningitis.
Section of Infectious Diseases, Department of Medicine, Kaohsiung Veterans General Hospital, 386, Ta-Chung 1st Road, Kaohsiung, 813, Taiwan. hctsai1011@yahoo.com.tw
Hung-Chin
HC
Tsai
Sheng-Fu
SF
Liu
Kuan-Sheng
KS
Wu
Yung-Ching
YC
Liu
Min-Hon
MH
Shi
Eng-Rin
ER
Chen
Chuan-Min
CM
Yen
Susan Shin-Jung
SS
Lee
Yen-Lin
YL
Huang
Yao-Shen
YS
Chen
D000368:Aged; D001483:Base Sequence; D016022:Case-Control Studies; D015331:Cohort Studies; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007710:Klebsiella Infections / Q000134:cerebrospinal fluid / Q000201:enzymology*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification; D008297:Male; D020780:Matrix Metalloproteinase 9 / Q000134:cerebrospinal fluid*; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000201:enzymology*; D008875:Middle Aged; D011446:Prospective Studies; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000134:cerebrospinal fluid
D017931:DNA Primers; D004269:DNA, Bacterial; D019715:Tissue Inhibitor of Metalloproteinase-1; D020780:Matrix Metalloproteinase 9