Polymorphisms within human cytomegalovirus chemokine (UL146/UL147) and cytokine receptor genes (UL144) are not predictive of sequelae in congenitally infected children.
Human cytomegalovirus (HCMV) viral chemokine, UL146, and TNF alpha-like receptor UL144 genes show a high degree of hypervariability in clinical isolates. These proteins are predicted to be immune modulators and may contribute to the pathogenesis of HCMV infections. We analyzed the UL146 and UL144 genetic variation of 51 HCMV isolates from congenitally infected children and 13 isolates from children in childcare. There was no statistically significant correlation between UL146 and UL144 genotypes and HCMV disease and/or sequelae. However, there were some groups that had a relatively large proportion of asymptomatic outcomes. These included UL146 group 8 (7/8 asymptomatic) and UL146 group 10 (3/3 asymptomatic). UL144 group B had 11/15 (73%) asymptomatic. UL146 and UL144 genes remained stable in serial isolates from children in daycare for intervals up to three years. These results indicate that most UL146 and UL144 genotypes do not predict clinical sequelae following congenital HCMV infections.
Heo
Jinho
J
The University of Tennessee, Department of Microbiology, 1414 Cumberland Ave., Knoxville, TN 37996, USA.
Petheram
Susie
S
Demmler
Gail
G
Murph
Jody R
JR
Adler
Stuart P
SP
Bale
James
J
Sparer
Tim E
TE
D000595:Amino Acid Sequence; D019743:Chemokines, CXC / Q000235:genetics*; D002648:Child; D002656:Child Day Care Centers; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D003586:Cytomegalovirus Infections / Q000151:congenital* / Q000503:physiopathology* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011110:Polymorphism, Genetic; D011237:Predictive Value of Tests; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics*
D019743:Chemokines, CXC; D004279:DNA, Viral; D008562:Membrane Glycoproteins; C119624:UL144 ORF protein, Human herpesvirus 5; D014764:Viral Proteins; C121017:viral chemokine CXC-1, Cytomegalovirus