Identification of aluminum species in an aluminum-accumulating plant, hydrangea (Hydrangea macrophylla), by electrospray ionization mass spectrometry.
The use of electrospray ionization mass spectrometry (ESI-MS) in negative ion mode was investigated as a direct probe for identifying Al species in Al-accumulating hydrangea (Hydrangea macrophylla) samples. Cell sap solutions of hydrangea leaves were purified using Sephadex G-10 liquid chromatography and each fraction was analyzed using ESI-MS and ESI-MS/MS to identify Al species. In hydrangea leaves, a 1:1 Al-citrate complex was found as [AlH(-1)cit](-) (m/z 215), where H(3)cit denotes citric acid. This result is consistent with that of Ma et al. who used (27)Al-NMR.
Department of Chemistry and Chemical Biology, Gunma University, Kiryu, Japan. hotta@chem-bio.gunma-u.ac.jp
Hiroki
H
Hotta
Qiang
Q
Wang
Mioko
M
Fukuda
Shoichi
S
Aizawa
Tomonari
T
Umemura
Kazutoshi
K
Sekizawa
Kin-ichi
K
Tsunoda
D000535:Aluminum / Q000032:analysis* / Q000737:chemistry* / Q000378:metabolism; D019343:Citric Acid / Q000737:chemistry; D004777:Environment; D031966:Hydrangea / Q000166:cytology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D010070:Oxalates / Q000737:chemistry; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D014617:Vacuoles / Q000378:metabolism
D010070:Oxalates; D019343:Citric Acid; D000535:Aluminum; C455846:aluminum citrate