[Isolation and identification of major steroidal saponins of Paris polyphylla Smith var stenophylla Franch].
OBJECTIVE
To research the steroidal saponins of Paris polyphylla Smith var stenophylla Franch for enriching the resource of Radix Paridis.


METHODS
Compounds were isolated by silica gel, macroporous adsorption resin,Sephadex LH-20 and RP-C18 column chromatography and the structures were elucidated on the basis of spectroscopic methods.


RESULTS
Five kinds of steroidal saponins were isolated and identified as follows: paris saponin I (1) Diosgenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-[alpha-L-arabinofuranosyl-(1 --> 4 )]-beta-D-Glucopyranoside, paris saponin V (2) Diosgenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranoside, paris saponin VI (3) Pennogenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranoside, paris saponin VII (4) Pennogenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 4)-alpha-L-rhamnopyranosyl- 1 --> 4)-[alpha-L-rh-amnopyranosyl-(1 --> 2)]-beta-D-glucopyranoside, paris saponin H (5) Pennogenin-3-O-alpha-L-rhamnopyranosyl-(1 --> 2)-[alpha-L-arabinofuranosyl-(1 --> 4)]-beta-D-Glucopyranoside.


CONCLUSIONS
All the five kinds of steroidal saponins were isolated from P. polyphylla. Smith var stenophylla Franch for the first time.
Pharmacognosy Staff Room, West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Hong-Xiang
HX
Yin
Dan
D
Xue
Nan
N
Bai
Cu
C
Chen
Yang
Y
Chen
Hao
H
Zhang
D004144:Diosgenin / Q000737:chemistry / Q000302:isolation & purification; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification*; D027343:Rhizome / Q000737:chemistry; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D013256:Steroids / Q000737:chemistry / Q000302:isolation & purification*
D010936:Plant Extracts; D012503:Saponins; D013256:Steroids; C113878:pennogenin 3-O-rhamnopyranosyl-1-4-rhamnopyranosyl-1-4-(rhamnopyranosyl-1-2)-glucopyranoside; C479299:diosgenin 3-O-(rhamnopyranosyl-1-2-(glucopyranosyl-1-3))glucopyranoside; D004144:Diosgenin