[Establishment of rabbit model of juvenile nonalcoholic steatohepatitis].
To establish a rabbit model of juvenile nonalcoholic steatohepatitis (NASH) for further study.



Twenty-eight New Zealand rabbit pups were fed with a high-fat diet (standard diet+10 % lard+2 % cholesterol) for 8 or 12 weeks as the two model groups, and 10 rabbits were fed with standard diet as the controls. Liver tissue samples were collected for Heamatoxylin-Eosin staining and pathological examination.



Typical histological hepatic lesions of NASH were observed in both model groups. Compared with control group, model groups showed a significant increase in serum ALT, AST, TG, TC levels (P <0.01), and decrease in serum adiponectin, IL-10 levels (P <0.05), meanwhile there was no significant difference between two model groups. TC and the degree of liver fatty infiltration were independent determinants of serum adiponectin level by stepwise multiple regression, beta=-1.33, P=0.006 and beta=-0.97, P=0.038, respectively, R square equal to 0.294.



The juvenile steatohepatitis rabbit model has been established and the level of adiponectin can partly reflect the severity of liver steatosis.
Fang
Yan-Lan
YL
Department of Endocrinology, The Affiliated Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Liang
Li
L
Fu
Jun-Fen
JF
D052242:Adiponectin / Q000097:blood*; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000097:blood / Q000378:metabolism / Q000473:pathology; D005260:Female; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D011817:Rabbits; D011897:Random Allocation
D052242:Adiponectin; D004041:Dietary Fats; D016753:Interleukin-10