[Study on antimicrobial activity of human hemoglobin and its fragments both in vitro and in vivo].
OBJECTIVE
To isolate human hemoglobin and its fragrments, compare their antimicrobial activity in vitro and pilot study of their antimicrobial activity in vivo.


METHODS
The alpha and beta chains of hemoglobin were separated by cation exchange chromatography and gel chromatography; The alpha and beta chains were cleaved by cyanogens bromide respectively. The cleaved fragments were purified by reverse phase high performance liquid chromatography. Antimicrobial activity of hemoglobin and its fragments was determined by agrose radial diffusion assay. After establishment of E. coli vaginal infection model, the rats were randomized into the experimental group (hemoglobin group) and the control group. The histologically pathological section was observed.


RESULTS
Hemoglobin, alpha/beta chain and their fragments had similar antibacterial activities in vitro, which were mainly against Gram-negative bacteria E. coli; except alpha1-32 had a comparatively lower activity. Antimicrobial activity in vivo: a comparison of the hemoglobin group with the matrix control group (no treatment after infection), the surface layer of vaginal stratified squamous epithelium was smoother, inflammatory cells were significantly reduced in the lamina propria and congestion was obviously decreased.


CONCLUSIONS
Human hemoglobin and its fragments had antibacterial activity in vitro, hemoglobin might relieve the inflammation of E. coil vaginal infection in rats moreover.
Zhou
Xin-E
XE
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Ouyang
Yun-Wei
YW
Pan
Xiao-Ling
XL
He
Yue-Dong
YD
Li
Heng
H
Deng
Lu-Xia
LX
Shi
Yan-E
YE
Peng
Yuan-Yuan
YY
Zhao
Yuan
Y
Huang
Ning
N
D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000187:drug effects*; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000382:microbiology; D005260:Female; D006454:Hemoglobins / Q000737:chemistry / Q000494:pharmacology*; D006801:Humans; D010446:Peptide Fragments / Q000302:isolation & purification / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014621:Vagina / Q000187:drug effects / Q000382:microbiology
D000900:Anti-Bacterial Agents; D006454:Hemoglobins; D010446:Peptide Fragments