Differential expression of LAR tyrosine phosphatase in the rat developing molar tooth germ.
OBJECTIVE
This study examined the molecules implicated in the cytodifferentiation of dental hard tissue cells.


METHODS
Rat pups at postnatal days 4, 7 and 10 were used. Differential display-polymerase chain reaction (DD-PCR), Western blot and immunofluorescent localisation were performed to search differentially expressed genes in tooth development.


RESULTS
Leukocyte-common antigen-related tyrosine phosphatase (lar-tp) was differentially detected between the rat maxillary 2nd and 3rd molar germs on postnatal day 10, which were at the dental hard tissue formation and cap/early bell developmental stages, respectively. Both the mRNA and protein expression levels of lar-tp were higher in the 3rd molar germs than in the 2nd. In addition, the levels in the 2nd molar germs at postnatal days 4, 7 and 10, which corresponded to the early/late bell, crown and root stages, respectively, decreased in a time dependent manner. The immunoreactivity against intracellular P-subunit of lar-tp was detected in the ameloblasts and odontoblasts as well as in the undifferentiated inner enamel epithelia and dental papilla cells. However, strong immunoreactivity against extracellular E-subunit was observed only in the undifferentiated inner enamel epithelia and dental papilla cells in the 3rd molar germs and in the stratum intermedium in the 2nd molar germs.


CONCLUSIONS
This is the first identification of lar-tp in the molar tooth development and suggests that this molecule may be involved in the cytodifferentiation of dental hard tissue cells.
Department of Oral Anatomy, Wonkwang University, Iksan, South Korea.
H J
HJ
Kim
O S
OS
Kim
S C
SC
Kim
J H
JH
Kang
J Y
JY
Jung
M S
MS
Kim
W J
WJ
Kim
H J
HJ
Park
J T
JT
Koh
E J
EJ
Lee
S H
SH
Kim
D000565:Ameloblasts / Q000201:enzymology; D000818:Animals; D015153:Blotting, Western / Q000379:methods; D018507:Gene Expression Regulation, Developmental; D015971:Gene Expression Regulation, Enzymologic; D008963:Molar / Q000033:anatomy & histology / Q000201:enzymology* / Q000254:growth & development; D009804:Odontoblasts / Q000201:enzymology; D009805:Odontogenesis / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2 / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods; D014083:Tooth Germ / Q000033:anatomy & histology / Q000201:enzymology* / Q000254:growth & development
D012333:RNA, Messenger; D054623:Receptor-Like Protein Tyrosine Phosphatases, Class 2