Application of Evicel to cavernous nerves of the rat does not influence erectile function in vivo.
OBJECTIVE
To evaluate the effect of the fibrin sealant, Evicel, on the neuroregulatory control of penile erections in an experimental rat model.


METHODS
Two groups of rats were used: sham-operated rats with exposure of the bilateral cavernous nerves (CNs) and application of saline vehicle (500 microL), and rats treated with direct application of Evicel (500 microL) bilaterally to the CNs. At 14 and 45 days after application of Evicel to the CNs, the CNs were stimulated to measure the in vivo erectile responses. Additionally, we evaluated the neuronal nitric oxide synthase immunoreactivity in the dorsal CNs of the penis and changes in the smooth muscle and collagen deposition in the penis using a trichrome stain.


RESULTS
Evicel application to the CNs did not have any detrimental effect on the neurogenic erectile responses in vivo at 14 or 45 days after application. The neuronal nitric oxide synthase expression in the dorsal CNs of the penis was unchanged after Evicel application at all points studied, and we saw no change in the histomorphometric analysis findings of smooth muscle and collagen deposition in the penis.


CONCLUSIONS
These data suggest that the hemostatic agent, Evicel, is safe in an experimental rat model of erection physiology, with no detrimental effects on neuroregulatory control of erection.
Department of Urology, James Buchanan Brady Urological Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA. tbivala1@jhmi.edu
Trinity J
TJ
Bivalacqua
Thomas J
TJ
Guzzo
Edward M
EM
Schaeffer
Milena A
MA
Gebska
Hunter C
HC
Champion
Arthur L
AL
Burnett
Mark L
ML
Gonzalgo
D000818:Animals; D004558:Electric Stimulation; D015718:Fibrin Tissue Adhesive / Q000494:pharmacology*; D006490:Hemostatics / Q000494:pharmacology*; D008297:Male; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D010410:Penile Erection / Q000187:drug effects* / Q000502:physiology; D010413:Penis / Q000187:drug effects* / Q000294:innervation* / Q000502:physiology; D011468:Prostatectomy; D051381:Rats; D017207:Rats, Sprague-Dawley
D015718:Fibrin Tissue Adhesive; D006490:Hemostatics; D052248:Nitric Oxide Synthase Type I