Construction of a first genetic map of distylous Turnera and a fine-scale map of the S-locus region.
As a prelude to discovery of genes involved in floral dimorphism and incompatibility, a genetic map of distylous Turnera was constructed along with a fine-scale map of the S-locus region. The genetic map consists of 79 PCR-based molecular markers (48 AFLP, 18 RAPD, 9 ISSR, 4 RAMP), 5 isozyme loci, one additional gene, and the S-locus, spanning a total distance of 683.3 cM. The 86 markers are distributed in 5 linkage groups, corresponding to the haploid chromosome number. Molecular markers tightly linked or co-segregating with the S-locus in an initial mapping population of 94 individuals were used to assay an additional 642 progeny to construct a map of the S-locus region. The fine-scale map consists of 2 markers (IS864a and RP45E9) flanking the S-locus at distances of 0.41 and 0.54 cM, respectively, and 3 additional markers (OPK14c, RP45G18, and RP81E18) co-segregating with the S-locus in the total mapping population of 736 individuals. The genetic map constructed will serve as a framework for localization of genes outside the S-locus affecting distyly, while molecular markers of the fine-scale map will be used to initiate chromosome walking to find the genes residing at the S-locus.
Labonne
J D J
JD
Department of Biology, York University, 4700 Keele Street, Toronto, ON M3J1P3, Canada.
Vaisman
A
A
Shore
J S
JS
D002874:Chromosome Mapping; D035264:Flowers / Q000235:genetics; D008040:Genetic Linkage; D005819:Genetic Markers; D011110:Polymorphism, Genetic; D029647:Turnera / Q000235:genetics*
D005819:Genetic Markers