Heme oxygenase-1 attenuates contact hypersensitivity induced by 2,4-dinitrofluorobenzene in mice.
Heme oxygenase (HO)-1 may have an important role in the resolution of T cell-mediated inflammation. The authors elucidated the role of the anti-inflammatory HO-1 in the pathogenesis of skin inflammation, using a mouse contact hypersensitivity (CHS) induced by 2,4-dinitrofluorobenzene (DNFB). Ear swelling was induced by challenge with DNFB, accompanied by infiltration of inflammatory cells in the challenged ear skin. DNFB challenge induced low levels of HO-1 mRNA and protein expression. Ear swelling induced by DNFB challenge was significantly reduced by topical treatment with cobalt protoporphyrin IX (CoPP), a HO-1 inducer, but exaggerated by blockage of HO-1 activity with tin protoporphyrin IX (SnPP), a HO-1 inhibitor. Similarly, the number of infiltrated cells in DNFB-challenged ear skin were reduced by CoPP but increased by SnPP. Our findings suggest that HO-1 plays an important role in CHS and is an important pharmacological target for the treatment of CHS.
Department of Microbiology and Immunology, Wonkwang University School of Medicine, Iksan, Republic of Korea.
Hyun-Ock
HO
Pae
Young
Y
Ae Ha
Kyu-Yun
KY
Chai
Hun-Taeg
HT
Chung
D000818:Animals; D003877:Dermatitis, Contact / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D004139:Dinitrofluorobenzene / Q000633:toxicity*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D007249:Inflammation / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051379:Mice; D017319:Photosensitizing Agents / Q000494:pharmacology; D011524:Protoporphyrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D012867:Skin / Q000201:enzymology* / Q000473:pathology
D008565:Membrane Proteins; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D012333:RNA, Messenger; C028025:protoporphyrin IX; D004139:Dinitrofluorobenzene; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse