Isolation, culture and characterization of endothelial cells from human hypertrophic scar.
Although the etiology underlying scar formation is not well understood, previous studies revealed that endothelial cells play a role in the pathogenesis of scar development. Recently, the authors developed a reliable technique to obtain endothelial cells from hypertrophic scars that involved separation of cells from the scar tissue matrix and isolation from other cell types. Using phase-contract and electron microscopy, the cells were observed to have a characteristic morphology consistent with cells of endothelial origin. The cells were further characterized as endothelial cells by assessment of endothelin (ET)-1 and intercellular adhesion molecule (ICAM) mRNA expression, and the presence of factor VIII antigen, CD34, CD31, and VE-cadherin. This isolation method provides a simple method for culturing endothelial cells from hypertrophic scar tissue and should prove useful for studying the role of endothelial cell involvement in scar formation.
Institute of Burn, Ruijin Hospital, Jiaotong University Medical School, Shanghai, The People's Republic of China.
Xi-Qiao
XQ
Wang
Ying-Kai
YK
Liu
Zhi-Gang
ZG
Mao
Chun
C
Qing
Shu-Liang
SL
Lu
Da-Zhong
DZ
Xu
D015703:Antigens, CD / Q000032:analysis; D018952:Antigens, CD34 / Q000032:analysis; D015415:Biomarkers / Q000032:analysis; D015820:Cadherins / Q000032:analysis; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D017439:Cicatrix, Hypertrophic / Q000473:pathology*; D042783:Endothelial Cells / Q000166:cytology* / Q000276:immunology / Q000648:ultrastructure; D019332:Endothelin-1 / Q000032:analysis; D004730:Endothelium, Vascular / Q000166:cytology / Q000276:immunology / Q000648:ultrastructure; D005169:Factor VIII / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D018189:Immunomagnetic Separation / Q000379:methods*; D018799:Intercellular Adhesion Molecule-1 / Q000032:analysis; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000032:analysis; D012333:RNA, Messenger / Q000032:analysis; D000099152:Cadherin 5
D015703:Antigens, CD; D018952:Antigens, CD34; D015415:Biomarkers; D015820:Cadherins; D019332:Endothelin-1; D005169:Factor VIII; D018799:Intercellular Adhesion Molecule-1; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D012333:RNA, Messenger; D000099152:Cadherin 5