Inducing gene expression of cardiac antioxidant enzymes by dietary phenolic acids in rats.
An increase in oxidative stress is suggested to be intimately involved in the pathogenesis of heart failure. Phenolic acids are widespread in plant foods; they contain important biological and pharmacological properties. This study evaluated the role of phenolic acids on the expression of antioxidant enzymes in the heart of male Sprague-Dawley rats. Gallic acid, ferulic acid and p-coumaric acid at a dosage of 100 mg kg(-1) body weight significantly increased the activities of cardiac superoxide dismutase, glutathione peroxidase (GPx) and catalase (CAT) as compared with control rats (P<.05). The changes in cardiac CuZnSOD, GPx and CAT mRNA levels induced by phenolic acids were similar to those noted in the enzyme activity levels. A significant (P<.05) increase in the GSH/GSSG ratio was observed in the heart of phenolic acid-treated rats. The heart homogenates obtained from rats that were administered phenolic acids displayed significant (P<.05) increases in capacity for oxygen radical absorbance compared with control rats. Immunoblot analysis revealed the increased cardiac total level of Nrf2 in phenolic acid-treated rats. Interestingly, phenolic acid-mediated antioxidant enzyme expression was accompanied by up-regulation of heme oxygenase-1. This study demonstrates that antioxidant enzymes in rat cardiac tissue can be significantly induced by phenolic acids following oral administration.
National Institute of Cancer Research, National Health Research Institutes, Miaoli County, Taiwan.
Chi-Tai
CT
Yeh
Li-Chien
LC
Ching
Gow-Chin
GC
Yen
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D003373:Coumaric Acids / Q000494:pharmacology*; D004032:Diet; D005707:Gallic Acid / Q000494:pharmacology*; D005978:Glutathione; D005979:Glutathione Peroxidase / Q000378:metabolism; D006321:Heart / Q000187:drug effects; D006419:Heme Oxygenase (Decyclizing) / Q000096:biosynthesis; D008297:Male; D009206:Myocardium / Q000201:enzymology*; D051267:NF-E2-Related Factor 2 / Q000096:biosynthesis; D018384:Oxidative Stress; D011422:Propionates; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D003373:Coumaric Acids; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D011422:Propionates; D005707:Gallic Acid; C004999:ferulic acid; D002374:Catalase; D005979:Glutathione Peroxidase; D006419:Heme Oxygenase (Decyclizing); C491523:Hmox1 protein, rat; D013482:Superoxide Dismutase; D005978:Glutathione; C495469:p-coumaric acid