Influence of ovariectomy on healing of autogenous bone block grafts in the mandible: a histomorphometric study in an aged rat model.
OBJECTIVE
The aim of this study was to evaluate quantitatively and qualitatively the influence of estrogen deficiency on autogenous bone block grafts in aged ovariectomized rats.


METHODS
Fifty 12-month-old female Wistar rats were used in the study. They were divided into 2 groups, an ovariectomized group and a sham-operated group. After 30 days the animals received autogenous block bone grafts on the angle of the mandible, harvested from the calvaria. The animals were euthanized at 7, 14, or 28 days postoperatively.


RESULTS
Histologic analysis showed that at 7 days postsurgery, the interface between graft and recipient site in the sham-operated group appeared filled by a granulation tissue with angiogenic activity, whereas the ovariectomized group still exhibited a blood clot and a granulation tissue in organization. On the 14th postoperative day, the interface in the sham-operated group was partially filled by newly formed bone establishing a union between the graft and the recipient site. The interface in the ovariectomized group was typically filled by granulation tissue with discrete osteogenic activity in most specimens. On the 28th postoperative day, the graft in the sham-operated group appeared histologically integrated to the mandible. However, the interface in the ovariectomized group appeared partially filled by newly formed bone, with areas of interposed connective tissue. The statistical analysis revealed that bone neoformation was significantly greater in the sham-operated group (57.41% at 14 days and 68.35 at 28 days) in comparison with the ovariectomized group (40.82% at 14 days and 53.09 at 28 days) at the 5% level.


CONCLUSIONS
The estrogen depletion caused by the ovariectomy hindered the healing process of autogenous block bone grafts placed in the mandibles of aged rats.
Department of Surgery and Integrated Clinic, Division of Periodontics, São Paulo State University Júlio de Mesquita Filho UNESP, Araçatuba, São Paulo, Brazil. dsluize@bol.com.br
Danielle Shima
DS
Luize
Alvaro Francisco
AF
Bosco
Samara
S
Bonfante
Juliano Milanezi
JM
de Almeida
D000375:Aging / Q000502:physiology; D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D016025:Bone Transplantation / Q000502:physiology*; D004967:Estrogens / Q000172:deficiency* / Q000502:physiology; D005260:Female; D008334:Mandible / Q000601:surgery*; D019647:Oral Surgical Procedures; D010052:Ovariectomy; D010053:Ovary / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D014945:Wound Healing / Q000502:physiology
D004967:Estrogens