Zinc finger-based knockout punches for zebrafish genes.
The ability to manipulate the genome is critical to develop and test hypotheses based on genetics. Knockdown strategies focused on RNAi and/or morpholinos are excellent genetic tools, but they come with substantial technical limitations. A new gene targeting approach employing synthetic zinc finger nuclease (ZFN) technology is a powerful and complementary approach to directly modify genetic loci for many diverse applications, notably enhancing Danio rerio (the zebrafish) as an experimental organism for understanding human disease. This ZFN-based technology to generate targeted knockouts in this aquatic animal opens the door to an array of new biological models of human disease and genetic testing.
Ekker
Stephen C
SC
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA. Ekker.Stephen@mayo.edu
D000818:Animals; D003851:Deoxyribonucleases / Q000235:genetics / Q000378:metabolism; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000502:physiology; D015027:Zebrafish / Q000235:genetics*; D016335:Zinc Fingers
D003851:Deoxyribonucleases