Potentiometric system for selective formate measurement and improvement of response characteristics by permeation of cells.
A formate-selective biocatalytic potentiometric electrode system based on whole cells Pseudomonas oxalaticus has been developed. Permeation of the Gram-negative Pseudomonas oxalaticus microbial cells by EDTA is shown to improve the response slope from 38 mV/decade to 49 mV/decade, resulting in increased sensitivity. Out of 13 possibly interfering compounds tested, only pyruvate and lactate showed moderate response.
Department of Chemistry, University of Delaware, Newark, Delaware 19716.
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Rechnitz