The stability of the oxidative stress marker, urinary 8-hydroxy-2'- deoxyguanosine (8-OHdG), when stored at room temperature.
We examined the stabilities of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) stored at room temperature (25 degrees C) for 24 h or at -80 degrees C for 800 days. Subjects were 19 males and 17 females aged 23-58 yr for the 24-h study, and 9 males and 4 females aged 24-54 yr for the 800-day study. We obtained information on the subjects by questionnaires and interviews. The level of urinary 8-OHdG was measured by HPLC using two-step separations. There were no significant changes of amount of urinary 8-OHdG under either storage conditions. We conclude that urine samples can be stored at 25 degrees C and below for 24 h, when the research purpose includes the determination of urinary 8-OHdG. Urinary 8-OHdG was also stable for over two years when stored at -80 degrees C.
National Institute of Occupational Safety and Health, Kawasaki, Japan.
Yuki
Y
Matsumoto
Yasutaka
Y
Ogawa
Rie
R
Yoshida
Ayako
A
Shimamori
Hiroshi
H
Kasai
Hisayoshi
H
Ohta
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000328:Adult; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000032:analysis / Q000493:pharmacokinetics / Q000652:urine; D004355:Drug Stability; D004356:Drug Storage; D005260:Female; D006801:Humans; D007407:Interviews as Topic; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D013048:Specimen Handling; D011795:Surveys and Questionnaires; D013696:Temperature
D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine