A functional F analogue of Autographa californica nucleopolyhedrovirus GP64 from the Agrotis segetum granulovirus.
The envelope fusion protein F of Plutella xylostella granulovirus is a computational analogue of the GP64 envelope fusion protein of Autographa californica nucleopolyhedrovirus (AcMNPV). Granulovirus (GV) F proteins were thought to be unable to functionally replace GP64 in the AcMNPV pseudotyping system. In the present study the F protein of Agrotis segetum GV (AgseGV) was identified experimentally as the first functional GP64 analogue from GVs. AgseF can rescue virion propagation and infectivity of gp64-null AcMNPV. The AgseF-pseudotyped AcMNPV also induced syncytium formation as a consequence of low-pH-induced membrane fusion.
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.
Feifei
F
Yin
Manli
M
Wang
Ying
Y
Tan
Fei
F
Deng
Just M
JM
Vlak
Zhihong
Z
Hu
Hualin
H
Wang
D000818:Animals; D016367:Baculoviridae / Q000378:metabolism; D002459:Cell Fusion; D002460:Cell Line; D015500:Chloramphenicol O-Acetyltransferase / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D006863:Hydrogen-Ion Concentration; D008561:Membrane Fusion; D008957:Models, Genetic; D009036:Moths / Q000821:virology; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000378:metabolism*; D011995:Recombination, Genetic; D018411:Spodoptera / Q000166:cytology; D014162:Transfection; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism*; D014760:Viral Fusion Proteins / Q000235:genetics / Q000378:metabolism*; D014771:Virion / Q000378:metabolism; D014779:Virus Replication
D014759:Viral Envelope Proteins; D014760:Viral Fusion Proteins; D049452:Green Fluorescent Proteins; D015500:Chloramphenicol O-Acetyltransferase