Novel peptide-based pepsin inhibitors containing an epoxide group.
1,2-Epoxy-3-(p-nitrophenoxy)propane (EPNP) is known to inhibit pepsin A and other aspartic proteinases by reacting with the active site aspartic acid residue(s). However, the reaction is considerably slow in general, and therefore, it is desirable to develop similar reagents that are capable of inhibiting these enzymes more rapidly. In the present study, we synthesized a series of novel inhibitors which have a reactive epoxide group linked with peptide by a hydrazide bond, with a general structure: Iva-L-Val-L-Val-(L-AA)(n)-N2H2-ES-OEt (n = 0 approximately 2) (Iva, isovaleryl; AA, bulky hydrophobic or aromatic amino acid residue; ES, epoxysuccinyl). These inhibitors were shown to inhibit porcine pepsin A remarkably faster than EPNP.
Department of Chemistry, Faculty of Science and Engineering, Aoyama Gakuin University, Sagamihara, Kanagawa, Japan.
Hisashi
H
Ito
Tomoko
T
Hirono
Yusuke
Y
Morita
Yoshitaka
Y
Nemoto
Yong-Tae
YT
Kim
Kenji
K
Takahashi
D000818:Animals; D004852:Epoxy Compounds / Q000737:chemistry / Q000494:pharmacology*; D015394:Molecular Structure; D010434:Pepsin A / Q000037:antagonists & inhibitors*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D011480:Protease Inhibitors / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D013552:Swine
D004852:Epoxy Compounds; D010455:Peptides; D011480:Protease Inhibitors; D010434:Pepsin A