Identification of two distinct bovine parainfluenza virus type 3 genotypes.
The partial gene sequencing of the matrix (M) protein from seven clinical isolates of bovine parainfluenza virus type 3 (BPIV-3), and the complete sequencing of a representative isolate (Q5592) was completed in this study. Nucleotide sequence analysis was initiated because of the failure of in-house BPIV-3 RT-PCR methods to yield expected products for four of the isolates. Phylogenetic reconstructions based on the nucleotide sequences for the M-protein and the entire genome, using all of the available BPIV-3 nucleotide sequences, demonstrated that there were two distinct BPIV-3 genotypes (BPIV-3a and BPIV-3b). These newly identified genotypes have implications for the development of BPIV-3 molecular detection methods and may also impact on BPIV-3 vaccine formulations.
Department of Primary Industries and Fisheries, Ritchie Building 64A, Level 3, C Wing, Research Road, St Lucia, QLD 4072, Australia.
Paul Francis
PF
Horwood
Department of Primary Industries and Fisheries, Ritchie Building 64A, Level 3, C Wing, Research Road, St Lucia, QLD 4072, Australia.
Jennifer Lillian
JL
Gravel
Department of Primary Industries and Fisheries, Ritchie Building 64A, Level 3, C Wing, Research Road, St Lucia, QLD 4072, Australia.
Timothy John
TJ
Mahony
D000818:Animals; D002417:Cattle; D016679:Genome, Viral; D005838:Genotype; D006801:Humans; D008969:Molecular Sequence Data; D029101:Parainfluenza Virus 3, Bovine / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D010253:Respirovirus Infections / Q000821:virology; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014763:Viral Matrix Proteins / Q000235:genetics*
D012367:RNA, Viral; D014763:Viral Matrix Proteins