Oxidative stress biomarkers in sporadic ALS.
We aimed to investigate oxidative stress biomarkers in a cross-sectional pilot study of 50 participants with sporadic ALS (SALS) compared to 46 control subjects. We measured urinary 8-oxodeoxyguanosine (8-oxodG), urinary 15-F(2t)-isoprostane (IsoP), and plasma protein carbonyl by ELISA methods. We also determined if ELISA measurement of 8-oxodG could be validated against measures from high-pressure liquid chromatography coupled with electrochemical detection, the current standard method. We found that 8-oxodG and IsoP levels adjusted for creatinine were significantly elevated in SALS participants. These differences persisted after age and gender were controlled in regression analyses. These markers are highly and positively correlated with each other. 8-oxodG measured by the two techniques from the same urine sample were positively correlated (p<.0001). Protein carbonyl was not different between SALS participants and controls. In conclusion, using ELISA, we confirmed that certain oxidative stress biomarkers were elevated in SALS participants. ELISA may be reliable and thus useful in epidemiology studies requiring large numbers of samples to determine the significance of increased oxidative stress markers in SALS. Further studies are required.
Eleanor and Lou Gehrig MDA/ALS Research Center, Columbia University Medical Center, New York, NY 10032, USA. hm264@columbia.edu
Hiroshi
H
Mitsumoto
Regina M
RM
Santella
Xinhua
X
Liu
Mikhail
M
Bogdanov
Jennifer
J
Zipprich
Hui-Chen
HC
Wu
Julie
J
Mahata
Mary
M
Kilty
Kate
K
Bednarz
Daniel
D
Bell
Paul H
PH
Gordon
Mady
M
Hornig
Mahsa
M
Mehrazin
Ali
A
Naini
M
M
Flint Beal
Pam
P
Factor-Litvak
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000368:Aged; D000690:Amyotrophic Lateral Sclerosis / Q000453:epidemiology* / Q000378:metabolism*; D015415:Biomarkers / Q000097:blood* / Q000652:urine*; D001798:Blood Proteins / Q000378:metabolism; D003430:Cross-Sectional Studies; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D015237:Dinoprost / Q000031:analogs & derivatives / Q000652:urine; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D010865:Pilot Projects; D017382:Reactive Oxygen Species
D015415:Biomarkers; D001798:Blood Proteins; D017382:Reactive Oxygen Species; C075750:8-epi-prostaglandin F2alpha; D000080242:8-Hydroxy-2'-Deoxyguanosine; D015237:Dinoprost; D003849:Deoxyguanosine