Expanded-bed adsorption utilizing ion-exchange resin to purify extracellular beta-galactosidase.
The application of expanded-bed ion-exchange resins allows the elimination of intermediary particulate separation steps like filtration or centrifugation prior to adsorption steps in enzyme-purification processes from crude fermentation broths. This work is concerned with the experimental evaluation data of a process related to the adsorption of an extracellular p-galactosidase from the fungi Scopulariopsis. The protein recovery in the ion-exchange resin Accell Plus QMA was accomplished using a continuous-monitoring method. The direct adsorption step was followed by a elution step with concentrated NaCl solutions aiming to improve the enzyme-specific activity. Experimental data for fixed and expanded bed were compared.
Pereira
J A
JA
Departamento de Technologia de Alimentos, Universidade Federal de Viçosa, Brazil.
Vieira E Rosa
P De T
Pde T
Pastore
G M
GM
Santana
C C
CC