Pitfalls in the assay of carboxymethylcellulase activity.
A purified endocellulase from Sclerotium rolfsii and a crude cellulase preparation from Trichoderma reesei are used to illustrate several pitfalls associated with the assay of carboxymethylcellulase activity and the subsequent attainment of linear enzyme dilution curves. It is shown that the nature of both the enzymes and the substrate make the assay unsuitable for use in the calculation of enzyme recovery and purity.
Department of Biochemistry, Box 375, Pietermaritzburg 3200, South Africa.
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Lindner
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Dennison
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Quicke