Evidence of shedding of porcine circovirus type 2 in milk from experimentally infected sows.
Detection of porcine circovirus type 2 (PCV2) was to evaluate the milk from experimentally infected sows using polymerase chain reaction (PCR) and virus isolation. Six pregnant sows were inoculated intranasally with PCV2 at 93 days of gestation, and milk samples were collected from all sows at 1, 3, 6, 9, 12, 15, 18, 21, 24, and 27 days of lactation. PCV2 was detected in milk as early as day 1 of lactation in all six sows. Thereafter, all infected sows remained positive by PCR for PCV2 in milk until 27 days of lactation. In addition, PCV2 itself was isolated from milk collected from a virus-infected sows. These results suggest that PCV2 may be shed in milk following infection of pregnant sows by the virus.
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, San 56-1, Shillim-dong, Gwanak-gu, Seoul 151-742, Republic of Korea.
Y
Y
Ha
K K
KK
Ahn
B
B
Kim
K-D
KD
Cho
B H
BH
Lee
Y-S
YS
Oh
S-H
SH
Kim
C
C
Chae
D000818:Animals; D018173:Circoviridae Infections / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000235:genetics / Q000502:physiology*; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005260:Female; D008892:Milk / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011897:Random Allocation; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D017201:Virus Shedding
D004279:DNA, Viral