[Establishment of rabbit model of juvenile nonalcoholic steatohepatitis].
To establish a rabbit model of juvenile nonalcoholic steatohepatitis (NASH) for further study.



Twenty-eight New Zealand rabbit pups were fed with a high-fat diet (standard diet+10 % lard+2 % cholesterol) for 8 or 12 weeks as the two model groups, and 10 rabbits were fed with standard diet as the controls. Liver tissue samples were collected for Heamatoxylin-Eosin staining and pathological examination.



Typical histological hepatic lesions of NASH were observed in both model groups. Compared with control group, model groups showed a significant increase in serum ALT, AST, TG, TC levels (P <0.01), and decrease in serum adiponectin, IL-10 levels (P <0.05), meanwhile there was no significant difference between two model groups. TC and the degree of liver fatty infiltration were independent determinants of serum adiponectin level by stepwise multiple regression, beta=-1.33, P=0.006 and beta=-0.97, P=0.038, respectively, R square equal to 0.294.



The juvenile steatohepatitis rabbit model has been established and the level of adiponectin can partly reflect the severity of liver steatosis.
Department of Endocrinology, The Affiliated Children's Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Yan-Lan
YL
Fang
Li
L
Liang
Jun-Fen
JF
Fu
D052242:Adiponectin / Q000097:blood*; D000818:Animals; D004041:Dietary Fats / Q000008:administration & dosage; D004195:Disease Models, Animal; D005234:Fatty Liver / Q000097:blood / Q000378:metabolism / Q000473:pathology; D005260:Female; D016753:Interleukin-10 / Q000097:blood; D008297:Male; D011817:Rabbits; D011897:Random Allocation
D052242:Adiponectin; D004041:Dietary Fats; D016753:Interleukin-10