[Frequency of identification of cytomegalovirus DNA in patient of Public Independent Central Hospital in Warsaw in years 2002-2006].
OBJECTIVE
The present study shows frequency of presence of cytomegalovirus DNA in patients of Public Independent Central Clinical Hospital in Warsaw in years 2002-2006.


METHODS
2486 clinical samples taken from 604 patients in years 2002-2006 were tested for the presence of HHV-5 DNA using the qualitative in-house PCR method and quantitative real-time PCR assay.


RESULTS
Positive results were obtained in 5,3% of all examinations made during described period. All of positive samples came from Department of Hematology, Oncology and Internal Medicine patients--most of them was after stem cell transplantation.


CONCLUSIONS
These findings underline the value of PCR methods used in current diagnostics procedures. The high level of sensitivity and rapidity provided by this assays is favorable for the use in the detection fo HHV-5 DNA in clinical specimens.
Katedra i Zakład Mikrobiologii Lekarskiej, Akademia Medyczna w Warszawie. dzieciatkowski@wp.pl
Tomasz
T
Dzieciatkowski
Maciej
M
Przybylski
Agata
A
Sulowska
Maja
M
Mucha
Mirosław
M
Luczak
D000293:Adolescent; D000328:Adult; D002648:Child; D002675:Child, Preschool; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000175:diagnosis* / Q000453:epidemiology*; D004279:DNA, Viral / Q000097:blood*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006779:Hospitals, Public / Q000706:statistics & numerical data*; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012189:Retrospective Studies; D012680:Sensitivity and Specificity; D014763:Viral Matrix Proteins / Q000097:blood
D004279:DNA, Viral; D014763:Viral Matrix Proteins