Fine structural survey of tyrosine hydroxylase immunoreactive terminals in the myenteric ganglion of the rat duodenum.
We have examined whether the noradrenergic neurons have direct synaptic projections to the myenteric ganglion neurons of the duodenum and the ultrastructure of their terminals by using immunogold-silver labeling for tyrosine hydroxylase. In the neuropil of the myenteric ganglia, about half of the axon terminals contained round clear vesicles and the rest of them contained pleomorphic clear vesicles. The sizes of axon terminals contacting the dendrites as a whole were 1.62+/-0.07microm. All axon terminals formed asymmetric synaptic contacts with dendrites or somata. Immunohistochemical study revealed that the tyrosine hydroxylase-immunoreactive nerve terminals were distributed throughout the ganglia and contained exclusively pleomorphic clear synaptic vesicles (about 20-80nm long). The tyrosine hydroxylase-immunoreactive terminals were generally large (1.99+/-0.07microm). A considerable number of the tyrosine hydroxylase-immunoreactive terminals made asymmetric synaptic contacts with small dendrites, spines or somata of the myenteric ganglion neurons. Serial ultrathin sections through the myenteric neurons revealed that about 16% of the total number of axosomatic terminals showed tyrosine hydroxylase immunoreactivity. These results indicated that the myenteric ganglion neurons of the duodenum receive direct synaptic projection of sympathetic noradrenergic neurons and that their terminals contain pleomorphic vesicles and form asymmetric synaptic contacts.
Department of Anatomy, Hyogo College of Medicine, Mukogawa, Nishinomiya, Hyogo 663-8501, Japan. tetsu@hyo-med.ac.jp
Tetsu
T
Hayakawa
Sachi
S
Kuwahara
Seishi
S
Maeda
Koichi
K
Tanaka
Makoto
M
Seki
D000818:Animals; D001369:Axons / Q000201:enzymology / Q000648:ultrastructure; D003712:Dendrites / Q000201:enzymology / Q000648:ultrastructure; D004386:Duodenum / Q000201:enzymology* / Q000294:innervation*; D007150:Immunohistochemistry; D008854:Microscopy, Electron; D009197:Myenteric Plexus / Q000201:enzymology* / Q000648:ultrastructure*; D009412:Nerve Fibers / Q000648:ultrastructure; D017729:Presynaptic Terminals / Q000201:enzymology* / Q000648:ultrastructure*; D051381:Rats; D017207:Rats, Sprague-Dawley; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*
D014446:Tyrosine 3-Monooxygenase