Simple quantification of lansoprazole and rabeprazole concentrations in human serum by liquid chromatography/tandem mass spectrometry.
A rapid, simple and highly sensitive method was developed for the quantitative determination of lansoprazole and rabeprazole concentrations in 20 microL of human serum using high-performance liquid chromatography/tandem mass spectrometry (LC/MS/MS). Analytes, along with an internal standard (lansoprazole deuterium derivatives), were separated using a mobile phase of acetonitrile/1mM ammonium formate (140/60, v/v) on a C18 analytical column and analyzed in the selected reaction-monitoring (SRM) mode. The lower limit of quantification was 0.25 ng/mL. A good linear response was observed for each analyte (from 0.25 ng to 2.5 microg/mL). This method was useful for therapeutic drug monitoring and pharmacokinetic studies.
Hishinuma
Takanori
T
Division of Pharmacotherapy, Graduate School of Pharmaceutical Sciences, Tohoku University, 6-3, Aoba, Aramaki, Sendai 980-8578, Japan.
Suzuki
Kaori
K
Yamaguchi
Hiroaki
H
Yamagishi
Hatsushi
H
Koike
Tomoyuki
T
Ohara
Shuichi
S
Shimosegawa
Toru
T
Mano
Nariyasu
N
Goto
Junichi
J
D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D064747:Lansoprazole; D008297:Male; D064750:Rabeprazole; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D064747:Lansoprazole; D064750:Rabeprazole