Cytoprotective activity of annphenone against oxidative stress-induced apoptosis in V79-4 lung fibroblast cells.
We have elucidated the cytoprotective effect of annphenone (2,4-dihyroxy-6-methoxy-acetophenone 4-O-beta-D-glucopyranoside) against oxidative stress-induced apoptosis. Annphenone scavenged intracellular reactive oxygen species (ROS) and increased antioxidant enzyme activities. It thereby prevented lipid peroxidation and DNA damage, which was demonstrated by the inhibition of the formation of thiobarbituric acid reactive substance (TBARS), inhibition of the comet tail and decreased phospho-H2A.X expression. Annphenone protected Chinese hamster lung fibroblast (V79-4) cells from cell death via the inhibition of apoptosis induced by hydrogen peroxide (H2O2), as shown by decreased apoptotic nuclear fragmentation, decreased sub-G1 cell population and inhibited mitochondrial membrane potential (Deltapsi) loss. Taken together, these findings suggest that annphenone exhibits antioxidant properties by inhibiting ROS generation and thus protecting cells from H2O2-induced cell damage.
Department of Foods and Nutrition, College of Natural Sciences, Duk Sung Women's University, Seoul 132-714, Republic of Korea.
Jung Sook
JS
Shin
Kyoung Ah
KA
Kang
Eun Sil
ES
Kim
Rui
R
Zhang
Mei Jing
MJ
Piao
Dong Ok
DO
Ko
Zhi Hong
ZH
Wang
Young Hee
YH
Maeng
Su Yong
SY
Eun
Sungwook
S
Chae
Ha Sook
HS
Chung
Jin Won
JW
Hyun
D000098:Acetophenones / Q000737:chemistry / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D002460:Cell Line; D006224:Cricetinae; D019610:Cytoprotection / Q000187:drug effects*; D004249:DNA Damage; D005347:Fibroblasts / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology; D016166:Free Radical Scavengers / Q000494:pharmacology; D005960:Glucosides / Q000737:chemistry / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008168:Lung / Q000166:cytology*; D018384:Oxidative Stress / Q000187:drug effects*
D000098:Acetophenones; D000975:Antioxidants; D016166:Free Radical Scavengers; D005960:Glucosides; C532791:annphenone; D006861:Hydrogen Peroxide