The role of acetyl-coenzyme a synthetase in Arabidopsis.
The acs1 knockout mutant that has a disruption in the plastidic acetyl-coenzyme A (CoA) synthetase (ACS; At5g36880) gene was used to explore the role of this protein and plastidic acetate metabolism in Arabidopsis (Arabidopsis thaliana). Disruption of the ACS gene decreased ACS activity by 90% and largely blocked the incorporation of exogenous (14)C-acetate and (14)C-ethanol into fatty acids. Whereas the disruption had no significant effect on the synthesis of bulk seed triacylglycerols, the acs1 plants were smaller and flowered later. This suggests that the pyruvate dehydrogenase bypass provided by the aerobic fermentation pathway that converts pyruvate to acetate and probably on to fatty acids is important to the plants during normal growth. The role of ACS in destroying fermentative intermediates is supported by the increased sensitivity of the acs1 mutant to exogenous acetate, ethanol, and acetaldehyde compared to wild-type plants. Whereas these observations suggest that flux through the aerobic fermentation pathway is important, the reason for this flux is unclear. Interestingly, acetate is able to support high rates of plant growth on medium and this growth is blocked in the acs1 mutant.
Department of Genetics, Development, and Cell Biology, Iowa State University, Ames, Iowa 50010, USA.
Ming
M
Lin
David J
DJ
Oliver
D000079:Acetaldehyde / Q000378:metabolism; D000106:Acetate-CoA Ligase / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000085:Acetates / Q000378:metabolism; D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002244:Carbon / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation / Q000502:physiology; D006038:Glyoxylates / Q000378:metabolism; D016254:Mutagenesis, Insertional; D011768:Pyruvate Dehydrogenase Complex / Q000378:metabolism
D000085:Acetates; D029681:Arabidopsis Proteins; D006038:Glyoxylates; D011768:Pyruvate Dehydrogenase Complex; D000431:Ethanol; D002244:Carbon; D000106:Acetate-CoA Ligase; D000079:Acetaldehyde; C031150:glyoxylic acid