Sensitization assays: monocyte-derived dendritic cells versus a monocytic cell line (THP-1).
Dendritic cells (DCs) play a critical role in the skin sensitization process of contact allergens. Many efforts have been made to develop in vitro sensitization tests that employ DCs, but more recently protocols were introduced that use cell lines other than DCs. The potential of the cell line THP-1 compared to monocyte-derived DCs (MoDCs) was evaluated using a known potent sensitizer 2,4-dinitrochlorobenzene (DNCB) and the terpenoid ascaridol (1,4-epodioxy-p-menth-2-ene), an ingredient present in oxidized tea tree oil. Activation of these cells was studied by estimation of the CD86 and CD54 cell surface expression. Overall, comparable results were found. The expression of CD86 was augmented by ascaridol in THP-1 and MoDCs, while the expression of CD54 was not reproducibly increased. These results encourage the further development of THP-1 cells as a short-term model for sensitization testing.
Tietze
Corinna
C
Department of Environmental Toxicology, University of Trier, Trier, Germany.
Blomeke
Brunhilde
B
D051940:B7-2 Antigen / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation; D002460:Cell Line; D000081005:Cyclohexane Monoterpenes; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology / Q000378:metabolism; D007509:Irritants / Q000633:toxicity; D009000:Monocytes / Q000166:cytology*; D039821:Monoterpenes / Q000633:toxicity*; D010545:Peroxides / Q000633:toxicity*; D023422:Skin Irritancy Tests
D051940:B7-2 Antigen; D000081005:Cyclohexane Monoterpenes; D007509:Irritants; D039821:Monoterpenes; D010545:Peroxides; D018799:Intercellular Adhesion Molecule-1; C043372:ascaridole