Characterization of Klebsiella pneumoniae type 1 fimbriae by detection of phase variation during colonization and infection and impact on virulence.
Klebsiella pneumoniae is recognized as an important gram-negative opportunistic pathogen. The ability of bacteria to adhere to host structures is considered essential for the development of infections; however, few studies have examined the influence of adhesion factors on K. pneumoniae virulence. In this study, we cloned and characterized the type 1 fimbria gene cluster of a clinical K. pneumoniae isolate. Although this cluster was not identical to the Escherichia coli type 1 fimbria gene cluster, an overall high degree of structural resemblance was demonstrated. Unique to the K. pneumoniae fim gene cluster is the fimK gene, whose product contains an EAL domain, suggesting that it has a role in regulation of fimbrial expression. Like expression of type 1 fimbriae in E. coli, expression of type 1 fimbriae in K. pneumoniae was found to be phase variable, and an invertible DNA element (fim switch) was characterized. An isogenic type 1 fimbria mutant was constructed and used to evaluate the influence of type 1 fimbriae in different infection models. Type 1 fimbriae did not influence the ability of K. pneumoniae to colonize the intestine or infect the lungs, but they were determined to be a significant virulence factor in K. pneumoniae urinary tract infection. By use of a PCR-based assay, the orientation of the fim switch during colonization and infection was investigated and was found to be all "off" in the intestine and lungs but all "on" in the urinary tract. Our results suggest that during colonization and infection, there is pronounced selective pressure in different host environments for selection of either the type 1 fimbriated or nonfimbriated phenotype of K. pneumoniae.
Department of Bacteriology, Mycology and Parasitology, Statens Serum Institut, Copenhagen, Denmark. cas@ssi.dk
Carsten
C
Struve
Martin
M
Bojer
Karen Angeliki
KA
Krogfelt
D000818:Animals; D002353:Carrier State / Q000382:microbiology; D003001:Cloning, Molecular; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology* / Q000648:ultrastructure; D017353:Gene Deletion; D023061:Gene Order; D005798:Genes, Bacterial; D007422:Intestines / Q000382:microbiology; D007710:Klebsiella Infections / Q000382:microbiology*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000472:pathogenicity* / Q000648:ultrastructure; D008168:Lung / Q000382:microbiology; D008969:Molecular Sequence Data; D005810:Multigene Family; D011995:Recombination, Genetic; D017422:Sequence Analysis, DNA; D017385:Sequence Homology; D014551:Urinary Tract / Q000382:microbiology; D014774:Virulence; D037521:Virulence Factors / Q000235:genetics / Q000502:physiology*
D004269:DNA, Bacterial; D037521:Virulence Factors