[Analysis of SNPs in partial exons and 3'UTR of CAPN1 in porcine].
In this study, Wild boar, Min pig and Yorkshire were used to investigate the effect of CAPN1 gene on lean meat percentage and tenderness. Seven pairs of primers for exons and 3'untranslated region of CAPN1 gene were designed according to pig sequences from GenBank. Polymorphisms within exons were detected by PCR-SSCP method. SNPs in 3'untranslated region were detected by DNA sequencing, and PCR-RFLP method was then developed to screen the population. Totally, 11 SNPs, of which 5 from exons including one missense mutation resulting in amino acid substitution of M/V at the position of 260, 4 from introns and 2 from 3'untranslated region, were identified. Population genetics analysis showed that the distribution of genotypes among Yorkshire, Min pig and Wild boar were extremely significant difference (P<0.01), while there were no significant difference between Min pig and Wild boar (P>0.05), and extremely significant difference existed between Min pig and Yorkshire (P<0.01). Association of polymorphisms with breed characteristics indicated that the mutations detected by P4, P6 primers and that resulting in the change of Hinf 1 recognition site may be related to lean meat percentage.
College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China. xiuqin163@163. com
Xiu-Qin
XQ
Yang
Hui
H
Liu
Li-Juan
LJ
Guo
Qing-Zhi
QZ
Guan
Yao
Y
Xu
Di
D
Liu
D020413:3' Untranslated Regions / Q000235:genetics*; D000818:Animals; D002154:Calpain / Q000235:genetics*; D005091:Exons / Q000235:genetics*; D005828:Genetics, Population; D005838:Genotype; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D018807:Polymorphism, Single-Stranded Conformational / Q000235:genetics; D034421:Sus scrofa; D013552:Swine
D020413:3' Untranslated Regions; D002154:Calpain