Two-step purification of low-content cellular protein using protein-imprinted polymers.
We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps.
Biochemical Section of Key Laboratory of Functional Polymer Materials, the Ministry of Education of China, Institute of Polymer Chemistry, Nankai University, 300071 Tianjin, People's Republic of China.
Yi
Y
Long
Xiaocui
X
Xing
Ruifang
R
Han
Yang
Y
Sun
Ying
Y
Wang
Zhuo
Z
Zhao
Huaifeng
H
Mi
D000818:Animals; D002457:Cell Extracts / Q000032:analysis*; D008099:Liver / Q000737:chemistry; D008863:Microspheres; D054802:Molecular Imprinting / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000138:chemical synthesis / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D013552:Swine
D002457:Cell Extracts; D011108:Polymers; D011506:Proteins; D012333:RNA, Messenger; D011092:Polyethylene Glycols; D011142:Polyvinyl Alcohol