CrAsH-quantum dot nanohybrids for smart targeting of proteins.
Smart nanohybrids were prepared by conjugation of CrAsH to hydrosoluble and biocompatible quantum dots (QDs). The resulting probes were shown to bind efficiently and selectively to Cys-tagged proteins. The interaction with the protein was detected by an increase of the fluorescence emission of CrAsH. While the latter faded rapidly under continuous excitation, emission of the QD remained unaffected. The persistent fluorescence of the QD should thus allow extended monitoring of the target protein.
CEA, iBiTecS, Service de Chimie Bioorganique et de Marquage, 91191 Gif sur Yvette, France.
Emilie
E
Genin
Olivier
O
Carion
Benoit
B
Mahler
Benoit
B
Dubertret
Nathalie
N
Arhel
Pierre
P
Charneau
Eric
E
Doris
Charles
C
Mioskowski
D001672:Biocompatible Materials / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D045663:Quantum Dots; D011994:Recombinant Proteins / Q000737:chemistry*
D001672:Biocompatible Materials; C518706:CrAsH compound; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D010743:Phospholipids; D011994:Recombinant Proteins; C024098:6-carboxyfluorescein; D003545:Cysteine