Assay of the high-alkaline proteinase from alkalophilic bacillus PB92 using a chromogenic tripeptide substrate.
The chromogenic tripeptide substrate, benzyloxycarbonylglycyl-L-prolyl-L-citrulline p-nitroanilide, is proposed for the assay of the high-alkaline proteinase, HAP-PB92, from an alkalophilic Bacillus. The assay method is sensitive, reproducible, and may be adapted for an automatic system.
Gray
C J
CJ
Department of Chemistry, University of Birmingham, P.O. Box 363 Birmingham, B15 2TT, United Kingdom.
Barker
S A
SA
Dhariwal
M S
MS
Sullivan
J M
JM