Minus charge stimulation prevents LPS-induced liver injury by reduction of nitric oxide.
The liver is one of the major target organs affected in sepsis that are usually accompanied with free radical formation. The use of minus charge for the prevention and cure of various radical related diseases is gaining wide importance in the medicinal field. Here, we investigate whether minus charge stimulation (MCS) inhibits nitric oxide (NO) production induced by lipopolysaccharide (LPS) in the mice liver. The survival rate was compared in LPS-treated group with MCS group. The liver NO radical was measured using electron spin resonance technique. Serum alanine transaminase (ALT) was estimated for liver injury. MCS significantly improved the survival rate of LPS-treated mice and inhibited increase of ALT in serum levels. MCS also reduced NO radical production significantly in the LPS-treated mice liver tissue. In conclusion, our results indicate that MCS prevents LPS-induced liver injury, which may be through the inhibition of liver NO radical production.
ITO Co., LTD., 3-3-3, Toyotama-Minami, Nerima-ku, Tokyo 176-8605, Japan.
Fujitoshi
F
Senga
Li
L
Yin
Hiroshi
H
Karasuno
Hirokazu
H
Ohtaki
Tomoya
T
Nakamachi
Kazue
K
Satoh
Seiji
S
Shioda