Expression and characterization of LTx2, a neurotoxin from Lasiodora sp. effecting on calcium channels.
Here, we described the expression and characterization of the recombinant toxin LTx2, which was previously isolated from the venomous cDNA library of a Brazilian spider, Lasiodora sp. (Mygalomorphae, Theraphosidae). The recombinant toxin found in the soluble and insoluble fractions was purified by reverse phase high-performance liquid chromatography (HPLC). Ca2+ imaging analysis revealed that the recombinant LTx2 acts on calcium channels of BC3H1 cells, blocking L-type calcium channels.
Laboratório de Biologia Celular e Molecular, Núcleo de Pesquisa em Ciências Biológicas, Departamento de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, MG 35400.000, Brazil.
A A
AA
Dutra
L O
LO
Sousa
R R
RR
Resende
R L
RL
Brandão
E
E
Kalapothakis
I M
IM
Castro
D000818:Animals; D002118:Calcium / Q000502:physiology; D015220:Calcium Channels / Q000187:drug effects / Q000502:physiology; D002460:Cell Line; D003001:Cloning, Molecular; D053496:Inositol 1,4,5-Trisphosphate Receptors / Q000096:biosynthesis; D051379:Mice; D009498:Neurotoxins / Q000096:biosynthesis* / Q000494:pharmacology*; D011994:Recombinant Proteins / Q000737:chemistry / Q000302:isolation & purification; D019837:Ryanodine Receptor Calcium Release Channel / Q000096:biosynthesis; D013111:Spider Venoms / Q000096:biosynthesis / Q000737:chemistry* / Q000494:pharmacology*; D013112:Spiders / Q000737:chemistry
D015220:Calcium Channels; D053496:Inositol 1,4,5-Trisphosphate Receptors; C532438:LTx2 neurotoxin, Lasiodora; D009498:Neurotoxins; D011994:Recombinant Proteins; D019837:Ryanodine Receptor Calcium Release Channel; D013111:Spider Venoms; D002118:Calcium