Environmental contaminants and redox status of coenzyme Q10 and vitamin E in Inuit from Nunavik.
The Inuit are heavily exposed to potentially prooxidant contaminants such as methylmercury (MeHg) and polychlorinated biphenyls (PCB) through their traditional diet. This diet is also an abundant source of n-3 polyunsaturated fatty acids (n-3 PUFA), selenium, and antioxidants, which might reduce cardiovascular risk. Although Inuit from Nunavik have low concentrations of plasma oxidized low-density lipoprotein (OxLDL) and elevated glutathione-related antioxidant defenses, the variance in OxLDL was predicted by PCB and blood glutathione, leaving the issue of contaminant-associated oxidative stress unresolved. The objective of the study was to assess oxidative stress in these Inuit by measuring the plasma concentrations and redox states of alpha-tocopherol and coenzyme Q10 (CoQ10), 2 sensitive biomarkers of oxidative stress, in relation to exposure. Plasma lipophilic antioxidants were determined by high-performance liquid chromatography-coupled electrochemical detection; and their relations to PCB, MeHg, n-3 PUFA, selenium, and OxLDL were assessed by multivariate analyses. Ubiquinol-10, ubiquinone-10, and ubiquinone-10 to CoQ10(total) ratio were elevated as compared with white populations but showed no associations with PCB, MeHg, or n-3 PUFA. Ubiquinol-10 (beta = .23, P = .007) and CoQ10(total) (beta = .27, P = .009) were predicted by blood selenium; and alpha-tocopherol, by PCB (beta = 4.12, P = .0002), n-3 PUFA (beta = 9.16, P = .02), and OxLDL (beta = 3.04, P = .05). Unexpectedly, the alpha-tocopheryl quinone to alpha-tocopherol ratio, in the reference range, was negatively predicted by PCB (beta = -0.41, P = .02). Using sensitive biomarkers of redox alterations, we found no evidence for MeHg- or PCB-associated oxidative stress in these Inuit. However, despite robust blood antioxidant defenses, the unusually elevated ubiquinone-10 to CoQ10(total) ratio (0.21 +/- 0.11) suggests some form of oxidative stress of unknown origin.
Bélanger
Marie-Claire
MC
Québec Lipid Research Centre, CHUL Research Centre, Centre Hospitalier Universitaire de Québec, Québec City, Québec, Canada G1V 4G2.
Mirault
Marc-Edouard
ME
Dewailly
Eric
E
Berthiaume
Line
L
Julien
Pierre
P
D000328:Adult; D000975:Antioxidants / Q000032:analysis; D002170:Canada; D002851:Chromatography, High Pressure Liquid; D004785:Environmental Pollutants / Q000032:analysis*; D004912:Erythrocytes / Q000737:chemistry; D005227:Fatty Acids / Q000097:blood; D005231:Fatty Acids, Unsaturated / Q000097:blood; D005260:Female; D006801:Humans; D004930:Inuit; D008077:Lipoproteins, LDL / Q000378:metabolism; D008297:Male; D008767:Methylmercury Compounds / Q000097:blood; D008875:Middle Aged; D010084:Oxidation-Reduction; D011078:Polychlorinated Biphenyls / Q000097:blood; D012044:Regression Analysis; D012643:Selenium / Q000097:blood; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000097:blood / Q000378:metabolism; D014810:Vitamin E / Q000378:metabolism*
D000975:Antioxidants; D004785:Environmental Pollutants; D005227:Fatty Acids; D005231:Fatty Acids, Unsaturated; D008077:Lipoproteins, LDL; D008767:Methylmercury Compounds; D014451:Ubiquinone; D014810:Vitamin E; D011078:Polychlorinated Biphenyls; C024989:coenzyme Q10; D012643:Selenium; C025204:Ubiquinone Q2