Structural and functional relationships in the hybrid cluster protein family: structure of the anaerobically purified hybrid cluster protein from Desulfovibrio vulgaris at 1.35 A resolution.
The hybrid cluster protein (HCP) from the sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough has been isolated and crystallized anaerobically. The phase problem was solved for a P2(1)2(1)2(1) crystal form using multiple-wavelength anomalous diffraction data collected in the vicinity of the Fe K absorption edge. Although the overall protein structure is essentially the same as that previously obtained, it shows that the nature of the hybrid cluster has particular differences when isolated and crystallized in the absence of oxygen and this provides insight into the structural features associated with changes in the oxidation state. A comparison between HCPs and carbon monoxide dehydrogenases (CoDs) shows that they possess a similar fold and that the dehydrogenases have a related cluster at the equivalent HCP hybrid cluster position. This helps to understand the nature of the hybrid cluster and to predict a dimeric structure for class 3 HCPs, which lack the N-terminal region.
Instituto de Tecnologia Química e Biológica, Avenida da Repúlica, Apartado 127, 2781-901 Oeiras, Portugal.
David
D
Aragão
Edward P
EP
Mitchell
Carlos F
CF
Frazão
Maria Arménia
MA
Carrondo
Peter F
PF
Lindley
D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000235:genetics; D000595:Amino Acid Sequence; D000693:Anaerobiosis; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D018360:Crystallography, X-Ray; D016969:Desulfovibrio vulgaris / Q000737:chemistry* / Q000235:genetics; D019281:Dimerization; D007506:Iron-Sulfur Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification; D008958:Models, Molecular; D008969:Molecular Sequence Data; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics; D010084:Oxidation-Reduction; D011487:Protein Conformation; D017510:Protein Folding; D020836:Protein Structure, Quaternary; D021122:Protein Subunits; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D017386:Sequence Homology, Amino Acid
D001426:Bacterial Proteins; D007506:Iron-Sulfur Proteins; D009097:Multienzyme Complexes; D021122:Protein Subunits; D011994:Recombinant Proteins; C075199:prismane protein, Desulfovibrio vulgaris; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase