Origin of CD11b+ macrophage-like cells in the CNS of PLP-overexpressing mice: low influx of haematogenous macrophages and unchanged blood-brain-barrier in the optic nerve.
We have recently reported that overexpression of proteolipid protein in oligodendrocytes leads to a pathologically relevant increase of both CD8+ T-lymphocytes and CD11b+ cells in the CNS. We now focussed on the origin of the CD11b+ cells in the optic nerve, a well established structure for the analysis of the mutant, using bone marrow chimeric mice. Although there is an age-related increase in CD11b+ cells in the myelinated part of the optic nerve of the mutants, the percentage of infiltrating cells was not increased, but enhanced proliferation was detectable. In the non-myelinated optic nerve head, the rate of infiltrating CD11b+ cells and albumin extravasation was high in both genotypes. However, albumin extravasation was also high in the rostral myelinated part, where CD11b+ cell influx was low. Our study demonstrates an intrinsic origin of CD11b+ cells in the presence of an unchanged blood-brain-barrier in a CNS myelin mutant.
Department of Neurology, Developmental Neurobiology, University of Wuerzburg, Josef-Schneider Str. 11, D-97080 Wuerzburg, Germany.
Chi Wang
CW
Ip
Bianca
B
Kohl
Christoph
C
Kleinschnitz
Bernhard
B
Reuss
Klaus Armin
KA
Nave
Antje
A
Kroner
Rudolf
R
Martini
D000818:Animals; D001812:Blood-Brain Barrier / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D016026:Bone Marrow Transplantation / Q000379:methods; D039481:CD11b Antigen / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics* / Q000276:immunology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D018991:Myelin Proteolipid Protein / Q000096:biosynthesis / Q000235:genetics*; D009900:Optic Nerve / Q000276:immunology / Q000378:metabolism / Q000473:pathology*
D039481:CD11b Antigen; D018991:Myelin Proteolipid Protein; C047516:Plp1 protein, mouse