The role of antibodies specific for toxic sPLA2s and haemorrhagins in neutralizing potential of antisera raised against Vipera ammodytes ammodytes venom.
The contribution of antibodies directed against the two main toxic groups of proteins in the Vipera ammodytes ammodytes venom, haemorrhagic metalloproteinases (H) and neurotoxic sPLA2s (Atxs), to the overall protective efficacy of the whole venom antisera was investigated. Using ELISA assays we established a high correlation between the protective efficacy of the whole venom antisera in mice and their anti-Atxs antibody content. As the haemorrhage is the prevailing toxic effect of the venom in human, the lack of correlation also with anti-H IgG content exposed that the mouse model might not be optimal to evaluate the neutralizing potential of the venom-specific antisera for human therapy. We further revealed that Atxs and structurally very similar but non-toxic AtnI2 from the venom are not immuno cross-reactive.
Research and Development Department, Institute of Immunology, Inc., Rockefellerova 10, HR-10 000 Zagreb, Croatia. bhalassy@imz.hr
Beata
B
Halassy
Lidija
L
Habjanec
Marija
M
Brgles
Maja Lang
ML
Balija
Adrijana
A
Leonardi
Lidija
L
Kovacic
Petra
P
Prijatelj
Jelka
J
Tomasić
Igor
I
Krizaj
D000818:Animals; D000918:Antibody Specificity; D000941:Antigens; D000997:Antivenins / Q000276:immunology / Q000494:pharmacology*; D003429:Cross Reactions; D004797:Enzyme-Linked Immunosorbent Assay; D054501:Group II Phospholipases A2 / Q000276:immunology; D007106:Immune Sera / Q000276:immunology / Q000494:pharmacology*; D007114:Immunization; D007928:Lethal Dose 50; D045726:Metalloproteases / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D051379:Mice; D009500:Neutralization Tests; D054497:Phospholipases A2, Secretory / Q000037:antagonists & inhibitors* / Q000276:immunology / Q000633:toxicity; D011817:Rabbits; D014757:Viper Venoms / Q000037:antagonists & inhibitors* / Q000201:enzymology / Q000276:immunology / Q000633:toxicity; D017819:Viperidae / Q000276:immunology*
D000941:Antigens; D000997:Antivenins; D007106:Immune Sera; D014757:Viper Venoms; C068117:ammodytoxin A; D054501:Group II Phospholipases A2; D054497:Phospholipases A2, Secretory; C060161:ammodytoxin C; D045726:Metalloproteases