A rice serine carboxypeptidase-like gene OsBISCPL1 is involved in regulation of defense responses against biotic and oxidative stress.
Serine carboxypeptidase-like proteins (SCPLs) comprise a large family of protein hydrolyzing enzymes that play roles in multiple cellular processes. During the course of study aimed at elucidating the molecular basis of induced immunity in rice, a gene, OsBISCPL1, encoding a putative SCPL, was isolated and identified. OsBISCPL1 contains a conserved peptidase S10 domain, serine active site and a signal peptide at N-terminus. OsBISCPL1 is expressed ubiquitously in rice, including roots, stems, leaves and spikes. Expression of OsBISCPL1 in leaves was significantly up-regulated after treatments with benzothiadiazole, salicylic acid, jasmonic acid and 1-amino cyclopropane-1-carboxylic acid, and also up-regulated in incompatible interactions between rice and the blast fungus, Magnaporthe grisea. Transgenic Arabidopsis plants with constitutive expression of OsBISCPL1 were generated and disease resistance assays indicated that the OsBISCPL1-overexpressing plants showed an enhanced disease resistance against Pseudomonas syringae pv. tomato and Alternaria brassicicola. Expression levels of defense-related genes, e.g. PR1, PR2, PR5 and PDF1.2, were constitutively up-regulated in transgenic plants as compared with those in wild-type plants. Furthermore, the OsBISCPL1-overexpressing plants also showed an increased tolerance to oxidative stress and up-regulated expression of oxidative stress-related genes. The results suggest that the OsBISCPL1 may be involved in regulation of defense responses against pathogen infection and oxidative stress.
State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Huajiachi Campus, Hangzhou, Zhejiang 310029, People's Republic of China.
Huizhi
H
Liu
Xiaoe
X
Wang
Huijuan
H
Zhang
Yayun
Y
Yang
Xiuchun
X
Ge
Fengming
F
Song
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics; D002268:Carboxypeptidases / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate / Q000276:immunology*; D020082:Magnaporthe; D008969:Molecular Sequence Data; D012275:Oryza / Q000276:immunology*; D018384:Oxidative Stress; D010935:Plant Diseases / Q000276:immunology*; D030821:Plants, Genetically Modified; D015854:Up-Regulation
D002268:Carboxypeptidases; C022633:serine carboxypeptidase