The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells.
We investigated the effect of a triterpene saponoside from Lysimachia thyrsiflora L. upon the viability, proliferation, morphology and cell motility of human melanoma HTB-140 cells and human skin fibroblasts (HSFs). The compound, denoted LTS-4, decreased the viability and rate of cell growth of both cell types in a time-and dose-dependent manner, and proved cytotoxic against cancer cells at significantly lower concentrations than for fibroblasts. LTS-4 also affected the morphology of the examined cells, causing vacuolisation and actin cytoskeleton disintegration, and had an inhibitory effect on the tumour cell motility.
Galanty
Agnieszka
A
Department of Pharmacognosy, Faculty of Pharmacy, Jagiellonian University, ul. Medyczna 9, 30-688, Kraków, Poland. mfgalant@cyf-kr.edu.pl
Michalik
Marta
M
Sedek
Lukasz
L
Podolak
Irma
I
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003599:Cytoskeleton / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008545:Melanoma / Q000378:metabolism*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D029623:Primulaceae / Q000737:chemistry; D012503:Saponins / Q000494:pharmacology*; D012867:Skin / Q000166:cytology*
C524508:3-O-(beta-D-xylopyranosyl-(1-2)-beta-D-glucopyranosyl-(1-4)-(beta-D-glucopyranosyl-(1-2))-alpha-L-arabinopyranosyl)cyclamiretin A; D012503:Saponins; D009828:Oleanolic Acid