Cell adhesion properties of patterned poly(acrylic acid)/poly(allylamine hydrochloride) multilayer films created by room-temperature imprinting technique.
Patterned poly(acrylic acid) (PAA)/poly(allylamine hydrochloride) (PAH) multilayer films with line structures of different lateral size and vertical height were fabricated by a room-temperature imprinting technique, and their cell adhesion properties were investigated. The nonimprinted PAA/PAH multilayer films are cytophilic toward NIH/3T3 fibroblasts and HeLa cells whether PAA or PAH is the outer most layer. In contrast, the PAA/PAH multilayer films with a 6.5-microm-line/3.5-microm-space pattern structure are cytophobic toward NIH/3T3 fibroblasts and HeLa cells when the height of the lines is 1.29 microm. By either increasing the lateral size of the patters to 69-microm-line/43-mum-space or decreasing the height of the imprinted lines to approximately 107 nm, imprinted PAA/PAH multilayer films become cytophilic. This kind of transition of cell adhesion behavior derives from the change of the physical pattern size of the PAA/PAH multilayer films and is independent of the chemical composition of the films. The easy patterning of layer-by-layer assembled polymeric multilayer films with the room-temperature imprinting technique provides a facile way to tailor the cellular behavior of the layered polymeric films by simply changing the pattern dimensions.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
Yingxi
Y
Lu
Junqi
J
Sun
Jiacong
J
Shen
D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D006801:Humans; D051379:Mice; D018625:Microscopy, Atomic Force; D054802:Molecular Imprinting; D011073:Polyamines / Q000737:chemistry*; D013696:Temperature
D000180:Acrylic Resins; D011073:Polyamines; C063994:polyallylamine; C006903:carbopol 940