Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli.
We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.
Department of Pediatrics, Children's Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, PA 15213, USA.
Joshua
J
Hill
Shilpa
S
Beriwal
Ishwad
I
Chandra
Vinod K
VK
Paul
Aarti
A
Kapil
Tripti
T
Singh
Robert M
RM
Wadowsky
Vinita
V
Singh
Ankur
A
Goyal
Timo
T
Jahnukainen
James R
JR
Johnson
Phillip I
PI
Tarr
Abhay
A
Vats
D001431:Bacteriological Techniques / Q000379:methods*; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics / Q000302:isolation & purification*; D004927:Escherichia coli Infections / Q000175:diagnosis*; D006801:Humans; D008969:Molecular Sequence Data; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012680:Sensitivity and Specificity; D014556:Urine / Q000382:microbiology
D017931:DNA Primers