Production and verification of human papillomavirus type 18 vaccine in vitro.
This study aimed to generate human papillomavirus (HPV) type 18 L1 virus-like particles (VLPs) and investigated the immunity effectiveness in vitro by using the Bac to Bac baculovirus expression system and Ni-NTA purification system to express and purify the VLP. The bio-function and immune response of purified VLP were analyzed by a mouse erythrocyte haemagglutination assay and the IFN-gamma ELISPOT. The proteins of interest, primarily located at the nucleus, formed VLP by self-assembly, and had an effective immune activity in vitro. The level of IFN-gamma in the specific HPV18 positive group was significantly higher than that of the mixed high-risk HPV infected group and non-HPV infection one in vitro. Our study indicated that HPV18 later protein L1 was efficiently expressed in Bac to Bac baculovirus expression system and the HPV18 L1-VLPs showed immunoreactivity and bio-activity in vitro. Furthermore, the ELISPOT results support the hypothesis that the cell-mediated immunity generated by HPV18 L1-VLP may cross-type among high-risk HPV types.
Liao
Shujie
S
Cancer Biology Research Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430030, P.R. China.
Wang
Shixuan
S
Xu
Ling
L
Deng
Dongrui
D
Xu
Qian
Q
Wang
Wei
W
Zhu
Tao
T
Bai
Xiangyang
X
Zhou
Jianfeng
J
Xu
Gang
G
Lu
Yunping
Y
Meng
Li
L
Ma
Ding
D
D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D002478:Cells, Cultured; D006386:Hemagglutination Tests; D007371:Interferon-gamma / Q000096:biosynthesis; D051379:Mice; D008810:Mice, Inbred C57BL; D053918:Papillomavirus Vaccines / Q000096:biosynthesis* / Q000276:immunology / Q000302:isolation & purification; D016133:Polymerase Chain Reaction; D018411:Spodoptera
D053918:Papillomavirus Vaccines; C492959:human papillomavirus type 18 L1 VLP vaccine; D007371:Interferon-gamma