Identification of amide protons of glutathione in MR spectra of tumour cells.
Signals attributable to amide protons and used in previous studies to measure intracellular pH were observed in the low-field region of the (1)H-MR spectra of four tumour cell lines: T98G, MCF-7, A172 and HeLa. The signals were more intense in the spectra of the two cell lines (T98G and MCF-7) characterised by higher concentrations of glutathione (GSH). After comparison with (1)H-MR spectra of GSH in solution at different pH values, the peaks were attributed to NHs of the Cys and Gly residues of GSH. Modification of the intracellular concentration of GSH by treatment with buthionine sulfoximine produced comparable decreases in the intensity of aliphatic signals of GSH and NHs under examination. The assignment was therefore confirmed.
Dipartimento di Tecnologie e Salute and INFN Gruppo Collegato Sanità, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Roma, Italy.
Sveva
S
Grande
Anna Maria
AM
Luciani
Antonella
A
Rosi
Laura
L
Guidoni
Vincenza
V
Viti
D000577:Amides / Q000032:analysis* / Q000737:chemistry; D014408:Biomarkers, Tumor / Q000032:analysis / Q000737:chemistry*; D045744:Cell Line, Tumor; D005978:Glutathione / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D009369:Neoplasms / Q000737:chemistry*; D011522:Protons
D000577:Amides; D014408:Biomarkers, Tumor; D011522:Protons; D005978:Glutathione