Study of Cbl-b dynamics in peripheral blood lymphocytes isolated from patients with multiple sclerosis.
E3 ubiquitin ligase Casitas B cell lymphoma-b (Cbl-b) has been recently highlighted as a negative regulator of T-cell activation and which dysfunction usually results in autoimmunity. To present, however, the possible involvement of Cbl-b in multiple sclerosis (MS), an autoimmune demyelinating disease mediated by T-helper 1 (Th1) cells is still unclear. To clarify this, using reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot analyses, we thus investigated the levels of Cbl-b mRNA and protein in peripheral blood T-lymphocytes isolated from 11 MS patients in acute relapse phase and 20 cases in remission phase. 16 healthy subjects were used as normal control. Cbl-b mRNA and protein levels were found both significantly down-regulated in peripheral blood lymphocytes isolated from MS patients (P<0.0001). Interestingly, this decrease of Cbl-b protein but not mRNA levels was significantly more marked in samples of relapsed patients than that of remitted cases (P<0.0001). In addition, it was shown that Cbl-b mRNA levels being inversely correlated with the frequency of MS clinical relapses (P<0.0001). Altogether, the data show for the first time that Cbl-b dynamics in peripheral blood T-lymphocyte subset and which possible relationship with the clinical onsets during MS.
Zhou
Wen-bin
WB
Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, Hunan, PR China.
Wang
Rui
R
Deng
Yong-ning
YN
Ji
Xiao-bei
XB
Huang
Guo-xiang
GX
Xu
Yuan-zhong
YZ
D000293:Adolescent; D000328:Adult; D002648:Child; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000097:blood* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009103:Multiple Sclerosis / Q000097:blood / Q000473:pathology*; D017711:Nonlinear Dynamics; D050721:Proto-Oncogene Proteins c-cbl / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism
D012333:RNA, Messenger; D050721:Proto-Oncogene Proteins c-cbl