PCR-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth.
OBJECTIVE
The aim of the present study was to evaluate the presence of the selected pathogens in samples from deciduous and permanent tooth root canals by using PCR method and to determine the association of these organisms with clinical symptoms.


METHODS
A total of 145 children, 5 to 13 years old, were involved in this study. The presence of selected pathogens (Actinomyces israelii, Candida albicans, Enterococcus faecalis, Fusobacterium nucleatum, Porphyromonas endodontalis, Porphyromonas gingivalis, Prevotella intermedia, Streptococcus intermedius, Treponema denticola, Parvimonas micra, Tannerella forsythensis, Enterococcus faecium, Prevotella melaninogenica) in infected root canals was studied using PCR.


RESULTS
T. denticola (P = .012, .02) and E. faecalis (P = .012, .04) were highly associated with periapical radiolucency and previous pain, while P. gingivalis was associated with tenderness to percussion in both deciduous and permanent teeth (P = .01, .015).


CONCLUSIONS
The results of the present study confirm that certain species of microorganisms are associated with clinical signs and symptoms of endodontic disease in both deciduous and permanent teeth.
Cogulu
Dilsah
D
Department of Pedodontics, Ege University, School of Dentistry, Bornova-Izmir, Turkey. dilsah.cogulu@ege.edu.tr
Uzel
Atac
A
Oncag
Ozant
O
Eronat
Cemal
C
D000293:Adolescent; D001421:Bacteria, Anaerobic / Q000235:genetics / Q000472:pathogenicity*; D001424:Bacterial Infections / Q000382:microbiology; D002648:Child; D002675:Child, Preschool; D004269:DNA, Bacterial / Q000032:analysis; D003786:Dental Pulp Cavity / Q000382:microbiology*; D019229:Dentition, Permanent; D013293:Enterococcus faecalis / Q000235:genetics / Q000472:pathogenicity; D006801:Humans; D010485:Periapical Periodontitis / Q000382:microbiology*; D016133:Polymerase Chain Reaction; D016966:Porphyromonas gingivalis / Q000235:genetics / Q000472:pathogenicity; D014094:Tooth, Deciduous; D048373:Treponema denticola / Q000235:genetics / Q000472:pathogenicity
D004269:DNA, Bacterial