Zinc aspartate alleviates lung injury induced by intestinal ischemia-reperfusion in rats.
BACKGROUND
Intestinal ischemia-reperfusion (II/R) induced acute lung injury is mediated by activated neutrophils and formation of free radicals. Several antioxidants have been shown to attenuate such remote organ injury. We studied the effects of zinc aspartate on lung injury induced by II/R in rats.


METHODS
Twenty-four Sprague-Dawley rats were randomized into three groups. Group I was the control. Animals in Groups II and III (II/R + zinc aspartate [ZA]) underwent 60 min of ischemia and 60 min of reperfusion, respectively. Rats in Group III also received 50 mg/kg zinc aspartate before 15 min of reperfusion. Lung tissue samples and bronchoalveolar lavage fluid were obtained to assess lung tissue myeloperoxidase (MPO), adenosine deaminase (ADA), xanthine oxidase (XO), glutathione peroxidase (GPx) activities, and nitric oxide (NO), malondialdehyde (MDA) levels. Also, the levels of MDA, NO, and MPO activity were determined in bronchoalveolar lavage fluid.


RESULTS
Compared with the control, lung tissue MDA, NO levels, and MPO, ADA, XO activities were markedly increased (P < 0.05), whereas GPx activity significantly decreased in the II/R group (P < 0.05). However, administration of ZA significantly reversed these effects by reducing the levels of MDA, NO, and decreasing MPO, ADA, XO activities (P < 0.05). In addition, ZA significantly increased GPx activity (P < 0.05). The activity of MPO and the levels of NO and MDA were found to be higher in bronchoalveolar lavage fluid in II/R group than the control (P < 0.05). Zinc aspartate significantly diminished MPO activity and the levels of NO and MDA compared with that of control rats (P < 0.05).


CONCLUSIONS
Our results indicate that zinc aspartate alleviates lung injury induced by II/R attributable to its antioxidant and antiinflammatory effects.
Department of Thoracic Surgery, Kahramanmaras Sutcu Imam University Medical School, Kahramanmaras, Turkey. drhasanturut@yahoo.com
Hasan
H
Türüt
Ergul Belge
EB
Kurutas
Ertan
E
Bulbuloglu
Alptekin
A
Yasim
Mesut
M
Ozkaya
Ahmet
A
Onder
Secil Simsek
SS
Imrek
D000243:Adenosine Deaminase / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001224:Aspartic Acid / Q000494:pharmacology*; D001992:Bronchoalveolar Lavage Fluid; D004195:Disease Models, Animal; D005609:Free Radicals / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D007422:Intestines / Q000098:blood supply*; D008168:Lung / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D055370:Lung Injury / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D009504:Neutrophils / Q000473:pathology; D009569:Nitric Oxide / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications* / Q000378:metabolism; D014969:Xanthine Oxidase / Q000378:metabolism; D015032:Zinc / Q000494:pharmacology*
D000975:Antioxidants; D005609:Free Radicals; D001224:Aspartic Acid; D009569:Nitric Oxide; D009195:Peroxidase; D005979:Glutathione Peroxidase; D014969:Xanthine Oxidase; D000243:Adenosine Deaminase; D015032:Zinc