The effects of the size of liposuction cannula on adipocyte survival and the optimum temperature for fat graft storage: an experimental study.
BACKGROUND
Determining the most advantageous size of liposuction cannula and injection needles in terms of adipocyte viability could help to increase fat graft survival. When recurrent injections are necessary, storing fat tissue which is harvested during the first operation could be a practical solution if it is stored at an appropriate temperature providing the highest amount of viable fat cells.


METHODS
Fat tissue was removed from the abdomen of 10 consecutive female patients by 6-, 4- and 2-mm-diameter liposuction cannulas. Fat tissue harvested with the 6mm cannula was injected through 14, 16 and 20 g needles and collected in separate tubes. An additional three tubes of fat samples were prepared from fat tissue obtained with the 6mm cannula to be stored at +4, -20 and -80 degrees C for 2 weeks. Viability of the fat grafts was evaluated by fat cell isolation with collagenase digestion and staining with supravital dye and counting adipocytes with a haemocytometer.


RESULTS
The viability of fat grafts harvested with the 6mm cannula was higher than grafts obtained with smaller cannulas. The viability of fat grafts injected through 14, 16 and 20 g needles were similar to each other. The viability of fat grafts stored at +4 degrees C was similar to fresh tissue whereas freezing fat grafts caused significant loss of viable adipocytes compared to fresh tissue.


CONCLUSIONS
The use of larger liposuction cannulas for fat tissue harvesting provides more viable fat grafts. A temperature of +4 degrees C could be proposed as an effective and easily available way of storing fat grafts for at least 2 weeks.
Erdim
Melike
M
Department of Plastic and Reconstructive Surgery, Marmara University School of Medicine, 34349 Istanbul, Turkey. ermelike@yahoo.com
Tezel
Erdem
E
Numanoglu
Ayhan
A
Sav
Aydin
A
D017667:Adipocytes / Q000502:physiology* / Q000637:transplantation; D000328:Adult; D002470:Cell Survival / Q000502:physiology*; D003080:Cold Temperature; D015925:Cryopreservation / Q000379:methods; D004867:Equipment Design; D005260:Female; D006801:Humans; D015187:Lipectomy / Q000295:instrumentation* / Q000379:methods; D008875:Middle Aged; D009339:Needles; D014021:Tissue Preservation; D020858:Tissue and Organ Harvesting / Q000295:instrumentation