High levels of granzyme B expression in invasive cervical carcinoma correlates to poor response to treatment.
The present study assessed, in cervical carcinoma, expression levels of seven immune response genes and sought correlation to response to treatment. The expression levels of CD28, CTLA4, ICOS, ICOSL, CD80 and CD86 and granzyme B genes were assessed by real-time RT-PCR in pre-treatment tumor fragments. During the six-month follow-up after treatment, 8 patients presented tumor and 10 survived free of tumor. The only gene whose expression levels were higher in patients with poor outcome (p = 0.03) was granzyme B. Further evaluation, in adequately powered prospective studies is warranted to confirm the data and to translate this observation to the clinical setting.
Immunogenetics Division, Pediatrics Department, Federal University of Sao Paulo, Sao Paulo, SP, Brazil. valeskabaguzman@hotmail.com.br
Valeska B
VB
Guzman
Ismael D C G
ID
Silva
Sylvia M F
SM
Brenna
Carmen R N
CR
Carvalho
Julisa C L
JC
Ribalta
Maria
M
Gerbase-Delima
D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000628:therapy; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D018122:B7-1 Antigen / Q000032:analysis; D051940:B7-2 Antigen / Q000032:analysis; D018106:CD28 Antigens / Q000032:analysis; D060908:CTLA-4 Antigen; D002294:Carcinoma, Squamous Cell / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000628:therapy; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D053804:Granzymes / Q000032:analysis* / Q000235:genetics; D006801:Humans; D060945:Inducible T-Cell Co-Stimulator Ligand; D060889:Inducible T-Cell Co-Stimulator Protein; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D012333:RNA, Messenger / Q000032:analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D016896:Treatment Outcome; D015854:Up-Regulation; D002583:Uterine Cervical Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000473:pathology / Q000628:therapy
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D018122:B7-1 Antigen; D051940:B7-2 Antigen; D018106:CD28 Antigens; C497964:CD86 protein, human; D060908:CTLA-4 Antigen; C556706:CTLA4 protein, human; C556692:ICOS protein, human; C498514:ICOSLG protein, human; D060945:Inducible T-Cell Co-Stimulator Ligand; D060889:Inducible T-Cell Co-Stimulator Protein; D012333:RNA, Messenger; C509213:GZMB protein, human; D053804:Granzymes