Flavonoid treatment of murine macrophages does not alter ingestion or intracellular survival of Listeria monocytogenes.
It was recently reported that the aryl hydrocarbon receptor (AhR) plays a role in innate immunity to Listeria monocytogenes infection in mice. In this study the authors analyzed whether incubation of murine macrophages with various flavonoids that have agonist or antagonist activity for the AhR (beta-naphthoflavone, alpha-naphthoflavone, quercetin, luteolin) affect the uptake and survival of L. monocytogenes. Using the RAW264.7 and J774 murine macrophage cell lines and bone marrow-derived macrophages, the authors found no significant difference between flavonoid-treated and control macrophages. They also found that macrophages incubated with flavonoids alone did not exhibit a significant increase in release of tumor necrosis factor (TNF)-alpha, a crucial cytokine in anti-Listeria resistance.
Department of Pathobiological Sciences, School of Veterinary Medicine, College of Agriculture and Life Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Lyndsey
L
Berryman
Charles J
CJ
Czuprynski
D000818:Animals; D002460:Cell Line; D005419:Flavonoids / Q000494:pharmacology*; D007113:Immunity, Innate / Q000187:drug effects; D008089:Listeria monocytogenes / Q000276:immunology*; D008088:Listeriosis / Q000188:drug therapy / Q000276:immunology* / Q000382:microbiology; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists / Q000037:antagonists & inhibitors / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
D005419:Flavonoids; D018336:Receptors, Aryl Hydrocarbon; D014409:Tumor Necrosis Factor-alpha