Experimental design for the production of tensio-active agent by Candida lipolytica.
The strategy of optimization using sequential factorial design was employed to enhance the tensio-active emulsifying agent produced by Candida lipolytica using soybean oil refinery residue as substrate. A full factorial design was used to evaluate the impact of three fermentation factors-amounts of refinery residue, glutamic acid and yeast extract. This allowed exclusion of the yeast extract. Full factorials designs were then sequentially used to optimize the levels of the residue and glutamic acid. The surface tension value was finally reduced to 25.29 mN/m. The maximum emulsifier activity using different substrates was within 40 h of cultivation. The surface tension of the cell-free broth containing the biosurfactant remained very stable during exposure to a wide range of pH (2-12), temperatures (0-120 degrees C) and salinity (2-10% NaCl). The combination of an industrial waste and a cheap substrate therefore seems to be very promising for the low-cost production of potent biosurfactant.
Rufino
Raquel D
RD
Universidade Federal de Pernambuco, Recife-Pernambuco, CEP 50.670-420, Brazil.
Sarubbo
Leonie A
LA
Neto
Benício B
BB
Campos-Takaki
Galba M
GM
D002175:Candida / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D045703:Emulsifying Agents / Q000378:metabolism*; D018698:Glutamic Acid / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D010461:Peptones / Q000378:metabolism; D012965:Sodium Chloride; D013024:Soybean Oil / Q000378:metabolism; D013500:Surface Tension; D013696:Temperature; D013997:Time Factors
D003470:Culture Media; D045703:Emulsifying Agents; D007220:Industrial Waste; D010461:Peptones; D018698:Glutamic Acid; D012965:Sodium Chloride; D013024:Soybean Oil