Molecular typing of clinical Staphylococcus aureus isolates from northern India using coagulase gene PCR-RFLP.
Molecular typing of total 84 Staphylococcus aureus clinical isolates was performed using coagulase gene PCR. Out of 84 S. aureus strains total 33 different types of S. aureus strains were prevalent in this hospital and community. Types 2-7 and 9 were the most prevalent S. aureus strains accounting for more than 53% of total isolates. This technique is relatively inexpensive and is simple to perform and analyze.
Tiwari
Hare Krishna
HK
Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India. hktiwari_2005@rediffmail.com
Sapkota
Darshan
D
Gaur
Abhishek
A
Mathuria
Jitendra Prasad
JP
Singh
Asim
A
Sen
Malay Ranjan
MR
D003030:Coagulase / Q000235:genetics*; D005798:Genes, Bacterial; D006801:Humans; D007194:India / Q000453:epidemiology; D016106:Methicillin Resistance / Q000235:genetics; D017720:Molecular Epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000201:enzymology / Q000235:genetics
D003030:Coagulase