The effect of elevated salinity on 'California' Mozambique tilapia (Oreochromis mossambicus x O. urolepis hornorum) metabolism.
California Mozambique tilapia (Oreochromis mossambicus x O. urolepis hornorum) are extremely saline tolerant and have been previously shown to reduce whole-animal oxygen consumption rate (MO(2)) upon exposures to salinities greater than that of seawater (SW). In this study tilapia were acclimated to 15, 30, 45, 60 and 75 g/L salinity for 1, 5, 14, or 28 days. There was little change in plasma osmolality or muscle water content in salinities below 60 g/L, and branchial Na(+), K(+)-ATPase (NKA) activity was low in 15 and 30 g/L relative to 60 and 75 g/L. When tilapia were exposed to 75 g/L, plasma osmolality and NKA activity were significantly increased within 5 days of exposure relative to those in 15 and 30 g/L, and remained elevated over the entire 28 days acclimation, indicating that short term salinity challenges (i.e., 5 days) are predictive of longer exposure durations in this species. MO(2) following transfer to 15 and 30 g/L was elevated, reflecting the high energy demand required for switching from a hyper- to a hypo-osmoregulatory strategy. The MO(2) of 60 g/L-exposed fish was significantly reduced at 1, 5, and 14 days, relative to 30 g/L-exposed fish; however by 28 days there were no significant differences. We investigated the potential for a metabolic basis for the salinity-induced MO(2) reduction, using forward stepwise linear regression to correlate enzyme activities of brain, liver, and kidney with MO(2). Brain NKA was correlated with MO(2) after 5 days (p<0.01, r(2)=0.944) and both brain NKA and hepatic total ATPase were correlated with the reduced MO(2) at 14 days (p=0.027, r(2)=0.980 and p=0.025, r(2)=0.780, respectively). These results may indicate a tissue-level metabolic suppression, which has not been previously described as a response to hypersaline exposure in fishes.
Sardella
Brian A
BA
Department of Zoology, University of British Columbia, Vancouver B.C., Canada. basardella@ucdavis.edu
Brauner
Colin J
CJ
D000064:Acclimatization; D000818:Animals; D001921:Brain / Q000201:enzymology; D001934:Branchial Region / Q000201:enzymology; D015536:Down-Regulation; D004734:Energy Metabolism; D005618:Fresh Water / Q000737:chemistry*; D007422:Intestines / Q000201:enzymology; D007668:Kidney / Q000201:enzymology; D007700:Kinetics; D008099:Liver / Q000201:enzymology; D009132:Muscles / Q000378:metabolism; D009994:Osmolar Concentration; D010101:Oxygen Consumption; D054712:Salinity; D012623:Seawater / Q000737:chemistry*; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D017210:Tilapia / Q000097:blood / Q000378:metabolism*; D014882:Water-Electrolyte Balance
D000254:Sodium-Potassium-Exchanging ATPase