Tumor-inhibitory effect and immunomodulatory activity of fullerol C60(OH)x.
The tumor-inhibitory effect of C60(OH)x was tested on the murine H22 hepatocarcinoma model. Doses of 0.2 and 1.0 mg kg(-1) body weight both showed significant antitumor activity with tumor inhibition rates of 31.9 and 38.4%, respectively, when mice were treated for 17 consecutive days. The damnification of liver was prominently reduced. Furthermore, histological examination indicated that an envelope of fibroblasts and lymphocytes was formed surrounding tumor tissues in the C60(OH)x-treated group, which inhibited the infiltration of tumor to the neighboring normal skeleton muscle tissues. To understand the antitumor mechanism, the immunomodulatory activity of C60(OH)x was investigated. The results indicate that C60(OH)x enhances the phagocytosis of peritoneal macrophages and elevates the activity of arginase and acid phosphatase in vivo. The tumor necrosis factor alpha production of C60(OH)x-treated macrophages also increases in vitro. These results suggest that C60(OH)x can enhance the innate immunity of tumor-bearing mice, and therefore inhibits growth of the tumor.
Beijing National Laboratory for Molecular Sciences, Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P.R. China.
Jiadan
J
Zhu
Zhiqiang
Z
Ji
Jing
J
Wang
Ronghua
R
Sun
Xiang
X
Zhang
Yang
Y
Gao
Hongfang
H
Sun
Yuanfang
Y
Liu
Zheng
Z
Wang
Aidong
A
Li
Jie
J
Ma
Tiancheng
T
Wang
Guang
G
Jia
Yiqun
Y
Gu
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D045744:Cell Line, Tumor; D005260:Female; D037741:Fullerenes / Q000494:pharmacology*; D006131:Growth Inhibitors / Q000494:pharmacology*; D007155:Immunologic Factors / Q000494:pharmacology*; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy* / Q000473:pathology; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains
D000970:Antineoplastic Agents; D037741:Fullerenes; D006131:Growth Inhibitors; D007155:Immunologic Factors; C496652:fullerol C60(OH)22; C408042:fullerol C60(OH)24