Gene expression response in target organ and whole blood varies as a function of target organ injury phenotype.
This report details the standardized experimental design and the different data streams that were collected (histopathology, clinical chemistry, hematology and gene expression from the target tissue (liver) and a bio-available tissue (blood)) after treatment with eight known hepatotoxicants (at multiple time points and doses with multiple biological replicates). The results of the study demonstrate the classification of histopathological differences, likely reflecting differences in mechanisms of cell-specific toxicity, using either liver tissue or blood transcriptomic data.
Lobenhofer
Edward K
EK
Cogenics, Division of Clinical Data, Inc, Morrisville, NC 27560, USA. elobenhofer@cogenics.com
Auman
J Todd
JT
Blackshear
Pamela E
PE
Boorman
Gary A
GA
Bushel
Pierre R
PR
Cunningham
Michael L
ML
Fostel
Jennifer M
JM
Gerrish
Kevin
K
Heinloth
Alexandra N
AN
Irwin
Richard D
RD
Malarkey
David E
DE
Merrick
B Alex
BA
Sieber
Stella O
SO
Tucker
Charles J
CJ
Ward
Sandra M
SM
Wilson
Ralph E
RE
Hurban
Patrick
P
Tennant
Raymond W
RW
Paules
Richard S
RS
D000818:Animals; D001769:Blood / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D008099:Liver / Q000293:injuries* / Q000378:metabolism*; D008297:Male; D051381:Rats; D043922:Toxicogenetics / Q000379:methods*