Promoter methylation of p16 and EDNRB gene in leukemia patients in Taiwan.
Both epigenetic and genetic alternations are involved in cancer formation. In this study, we have identified the methylation frequency of p16 and endothelin receptor type B (EDNRB) of 26 leukemia patients and 8 randomly selected normal blood donors in Taiwan. Promoter methylation of p16 was detected in 85% of acute lymphocytic leukemia (ALL), 83% in acute myeloid leukemia (AML) whereas no methylation was detected in chronic myeloid leukemia (CML) in blast crisis. Hypermethylation of EDNRB was observed in 92% of ALL, 75% AML and 100% in CML in blast crisis. No aberrant methylation of p16 and EDNRB was found in 8 normal blood donors. Taken together, aberrant methylation of p16 and EDNRB was highly prevalent in leukemia patients in Taiwan.
Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan, Republic of China.
Pei-Ching
PC
Hsiao
Min-Chang
MC
Liu
Li-Mien
LM
Chen
Ching-Yi
CY
Tsai
Yu-Ting
YT
Wang
Jung
J
Chen
Li-Sung
LS
Hsu
D019175:DNA Methylation; D019942:Genes, p16; D006801:Humans; D007938:Leukemia / Q000235:genetics* / Q000473:pathology; D011401:Promoter Regions, Genetic; D044023:Receptor, Endothelin B / Q000235:genetics*
D044023:Receptor, Endothelin B