Use of a recycle-type SEC method as a powerful tool for purification of thiosialoside derivatives.
An efficient separation between fully acetylated thiosialoside methyl esters and fully acetylated Neu5Ac2en methyl esters was accomplished by means of a size-exclusion chromatography (SEC) method. Purity determinations and structural elucidation of the isolated compounds were performed by a combination of elemental analyses and spectroscopic analyses, including IR, (1)H, and (13)C NMR, and mass spectroscopic analyses.
Division of Material Science, Graduate School of Science and Engineering, Saitama University, Sakura, Saitama 338-8570, Japan.
Jun-Ichi
J
Sakamoto
Chiharu
C
Takita
Tetsuo
T
Koyama
Ken
K
Hatano
Daiyo
D
Terunuma
Koji
K
Matsuoka
D002236:Carbohydrate Conformation; D002850:Chromatography, Gel; D054330:Thiosugars / Q000737:chemistry / Q000302:isolation & purification*
D054330:Thiosugars