Enantioselective resolution of racemic styrene oxide at high concentration using recombinant Pichia pastoris expressing epoxide hydrolase of Rhodotorula glutinis in the presence of surfactant and glycerol.
The reaction medium was optimized to accomplish epoxide hydrolase-catalyzed, batch enantioselective hydrolysis of racemic styrene oxide at high initial substrate concentrations. The recombinant Pichia pastoris containing the epoxide hydrolase gene of Rhodotorula glutinis was used as the biocatalyst. Enantiopure (S)-styrene oxide with 98% ee was obtained with 41% yield (maximum yield = 50%) from 1.8 M racemic styrene oxide at pH 8.0, 4 degrees C in the presence of 40% (v/v) Tween 20 and 5% (v/v) glycerol.
Department of Chemical Engineering, Pusan National University, Busan 609-735, South Korea.
Seung Sik
SS
Yoo
Sunghoon
S
Park
Eun Yeol
EY
Lee
D004851:Epoxide Hydrolases / Q000235:genetics / Q000378:metabolism*; D004852:Epoxy Compounds / Q000737:chemistry / Q000302:isolation & purification*; D005990:Glycerol / Q000737:chemistry*; D010843:Pichia / Q000235:genetics*; D011995:Recombination, Genetic; D012248:Rhodotorula / Q000201:enzymology*; D013237:Stereoisomerism; D013501:Surface-Active Agents / Q000737:chemistry*
D004852:Epoxy Compounds; D013501:Surface-Active Agents; C013690:styrene oxide; D004851:Epoxide Hydrolases; D005990:Glycerol