Cell cycle arrest effects of large-dose FTY720 on lymphocytes in mouse skin transplantation models.
To probe into the mechanism of immunosuppression of FTY720, the authors study the cell cycle arrest effects of large-dose FTY720 on lymphocytes in mouse skin transplantation models and the expression of cell cycle-related factors in cell cycle regulation system in mouse skin allograft models using flow cytometry, Immunohistochemical staining, and reverse transcriptase-polymerase chain reaction (RT-PCR). FTY720 could prolong survival days of graft in mouse skin transplantation models obviously (p < 0.01). In thymus gland and lymph node, compared with the control group, cell percentage in G0-G1 increases and cell percentage in G2-M and S decreases in the treated group (p < 0.01). Expression of P16 increases and expression of cyclin D1 decreases in the treated group (p < 0.05). In thymus gland, it is shown by semiquantitative RT-PCR that the quantity of mRNA of P16 increases in the treated group (p < 0.01), and the quantity of mRNA of cyclin D1 decreases in the treated group (p < 0.01). FTY720 is a kind of effective immunosuppressant. FTY720 could induce cell cycle arrest in thymus gland and lymph node and change the expression of protein and the transcription of mRNA of cell cycle-related factor.
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Department of Urology and Transplantation, First Affiliated Hospital, Binzhou Medical University, Binzhou City, Shandong Province, China. Road, Binzhou City, Shandong Province 256603, China. lqy999496@yahoo.com.cn
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D000818:Animals; D002453:Cell Cycle / Q000187:drug effects* / Q000276:immunology; D056741:Cyclin D; D019941:Cyclin-Dependent Kinase Inhibitor p16 / Q000096:biosynthesis / Q000276:immunology; D016213:Cyclins / Q000096:biosynthesis / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D000068876:Fingolimod Hydrochloride; D005786:Gene Expression Regulation / Q000187:drug effects / Q000276:immunology; D006085:Graft Survival / Q000187:drug effects* / Q000276:immunology; D007166:Immunosuppressive Agents / Q000494:pharmacology*; D008214:Lymphocytes / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008954:Models, Biological; D011409:Propylene Glycols / Q000494:pharmacology*; D016038:Skin Transplantation / Q000276:immunology*; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D014184:Transplantation, Homologous
D056741:Cyclin D; D019941:Cyclin-Dependent Kinase Inhibitor p16; D016213:Cyclins; D007166:Immunosuppressive Agents; D011409:Propylene Glycols; D000068876:Fingolimod Hydrochloride; D013110:Sphingosine