Colifagina, a novel preparation of 8 lysed bacteria ameliorates experimental colitis.
Immune reactivity towards the bacterial intestinal flora plays an important part in the pathogenesis of inflammatory bowel disease. Administration of probiotic bacteria has beneficial effects on infectious and inflammatory diseases, principally in bowel disorders. However, little is known about the administration of soluble bacterial antigens in intestinal inflammation. We investigated the therapeutic effects of colifagina in experimental colitis. To assess this effect, C57BL/6 mice with dextran sulphate sodium-induced colitis were treated with colifagina, or with a placebo, for a period of 10 days. The mice were monitored, and inflammation was assessed by disease activity index (DAI). Analysis of fecal IgA concentration and measurement of IgA and inflammatory chemokine production in organ colonic culture was performed by ELISA. Clinically and histologically, bacterial-lysate-treated mice revealed significantly fewer DAI and a reduction of colonic histological inflammation. Treatment of healthy mice with colifagina significantly increased the fecal concentration of IgA and IgA production in organ culture. Colifagina administration in DSS-treated mice significantly increased the fecal concentration of IgA and IgA production in organ culture. MIP-1, MIP-2 and RANTES concentrations in colonic organ culture were significantly lower in colifagina-treated mice than in the placebo group. The use of colifagina is effective in amelioration of murine colitis.
Laboratory of Immunology and Inflammation and Division of Gastroenterology, Istituto Clinico Humanitas, Viale Manzoni, Rozzano, Milan, Italy.
S
S
Vetrano
C
C
Correale
E M
EM
Borroni
N
N
Pagano
B
B
Savino
M
M
Locati
A
A
Malesci
A
A
Repici
S
S
Danese
D000818:Animals; D018925:Chemokines / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000473:pathology; D005243:Feces / Q000382:microbiology; D007070:Immunoglobulin A / Q000276:immunology; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009924:Organ Culture Techniques; D019936:Probiotics / Q000627:therapeutic use*
D018925:Chemokines; D007070:Immunoglobulin A