Self-assembled monolayer for toxicant detection using nucleic acid sensor based on surface plasmon resonance technique.
Double stranded calf thymus deoxyribonucleic acid (dsCT-DNA) has been covalently immobilized onto self-assembled monolayer (SAM) of beta-merceptoethanol (MCE) on gold substrates via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry for fabrication of the surface plasmon resonance (SPR) based biosensing device. The dsCT-DNA-MCE-SAM/Au bioelectrode has been characterized using electrochemical impedance spectroscopy, cyclic voltammetry, contact angle measurements and atomic force microscopy, respectively. This biosensing device has been utilized for detection of cypermethrin (0.0005 ppm) using electrochemical and SPR techniques, respectively. The mechanism of interaction of cypermethrin with dsCT-DNA immobilized onto MCE-SAM has been proposed.
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr K. S. Krishnan Road, New Delhi, 110012, India.
Pratima R
PR
Solanki
Nirmal
N
Prabhakar
M K
MK
Pandey
B D
BD
Malhotra
D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002234:Carbodiimides / Q000737:chemistry; D002417:Cattle; D003432:Cross-Linking Reagents / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D017097:Electric Impedance; D004563:Electrochemistry / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry / Q000378:metabolism; D007202:Indicators and Reagents / Q000737:chemistry / Q000378:metabolism; D007306:Insecticides / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D018625:Microscopy, Atomic Force; D015394:Molecular Structure; D009696:Nucleic Acids / Q000737:chemistry / Q000378:metabolism*; D011722:Pyrethrins / Q000032:analysis* / Q000737:chemistry / Q000633:toxicity; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013379:Substrate Specificity; D013388:Succinimides / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D013499:Surface Properties
C518491:1-ethyl-3-(3-(diethylamino)propyl)carbodiimide; D002234:Carbodiimides; D003432:Cross-Linking Reagents; D007202:Indicators and Reagents; D007306:Insecticides; D009696:Nucleic Acids; D011722:Pyrethrins; D013388:Succinimides; C017160:cypermethrin; D006046:Gold; D004247:DNA; C427203:calf thymus DNA; C001426:N-hydroxysuccinimide