Dynamic interfacial properties of poly(ethylene glycol)-modified ferritin at the solid/liquid interface.
Poly(ethylene glycol)-modified ferritins (PEG-ferritins) with various molecular weights were synthesized by the grafting method, and their dynamic interfacial properties at the solid/liquid interface were investigated. The number of PEG grafted to ferritins was controlled by the amount of 1,1'-carbonyldiimidazole-modified PEG adding to the reaction solution. The adsorption kinetics and energy dissipation of PEG-ferritins onto bare Si substrate and amino-modified Si substrate were investigated with a quartz crystal microbalance (QCM) in 10 mM bis-Tris/HCl buffer (pH 5.8), while their morphologies were characterized by scanning electron microscopy (SEM). The adsorption dynamics of PEG-ferritins onto amino-modified Si substrate were quite different from those of unmodified ferritin, which can be reasonably interpreted by the desorption capability of PEG-ferritins on the surface attributed to amphiphilicity and the high-chain mobility of PEG chains.
Department of Electronic Chemistry, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan.
Yoshikazu
Y
Kumashiro
Yasuhiro
Y
Ikezoe
Kaoru
K
Tamada
Masahiko
M
Hara
D000327:Adsorption; D005293:Ferritins / Q000737:chemistry*; D007700:Kinetics; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D011092:Polyethylene Glycols / Q000737:chemistry*; D013053:Spectrophotometry; D013499:Surface Properties
D011092:Polyethylene Glycols; D005293:Ferritins