Expression and production of human interleukin-7 in insect cells using baculovirus expression vector system (BEVS).
Interleukin-7 (IL-7) is a glycoprotein cytokine with significant clinical and biomedical potential, such as cancer therapy and HIV infections. Earlier it has been cloned and expressed in various protein expression systems; however, they are not efficient for large-scale production. To address this inadequacy, we report in this paper the production of recombinant human interleukin-7 (hIL-7) in insect cells. A recombinant bacmid containing hIL-7 was constructed, purified, and characterized. It was used to infect Trichoplusia ni (BT1-TN-5B1/High Fivetrade mark) insect cells. Result shows that T. ni cells successfully produce hIL-7 in shake flask cultures. A scale up to 2.5-L laboratory batch bioreactor showed the efficacy of this system for large-scale production. Our results offer a highly efficient, inexpensive, and convenient system for the large-scale expression and production of recombinant hIL-7.
Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada.
Maryam
M
Mirzaei
Barbara
B
Jardin
Cynthia B
CB
Elias
Satya
S
Prakash
D000818:Animals; D016367:Baculoviridae / Q000235:genetics*; D002460:Cell Line; D003001:Cloning, Molecular; D005822:Genetic Vectors / Q000235:genetics*; D006801:Humans; D015851:Interleukin-7 / Q000235:genetics* / Q000378:metabolism*; D009036:Moths / Q000235:genetics* / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014162:Transfection / Q000379:methods*
D015851:Interleukin-7; D011994:Recombinant Proteins