Universal Reference RNA is not a representative normal sample for oligonucleotide microarray studies.
Translational research has been defined as the scientific study using human material that will ultimately generate patient specific data. A major caveat in human directed study is the availability of high quality and quantities of patient derived homogeneous cells for analysis. Whereas there exist sources for which tumor tissue and blood samples can be made available, the same cannot be said for normal tissue. The absence of normal control tissue has led to the creation of pooled cell lines and tissues for purchase known as "reference RNA". Although initially created for purposes of standardization, the difficulty associated with acquiring normal tissue has led some investigators to use sources of universal pooled RNA for comparative analysis with clinical tissue specimens. In order to study the effects of using Universal Reference RNA on expression profiling experiments we have evaluated the performance of universal RNA compared to RNA obtained from a purified population of colon epithelial cells in defining a set of altered transcripts in colon cancer.
Mojica
Wilfrido D
WD
Department of Pathology, University at Buffalo, State University of New York, Buffalo, NY 14203, USA. mojica@buffalo.edu
Stein
Leighton
L
Hawthorn
Lesleyann
L
D045744:Cell Line, Tumor; D003106:Colon / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling / Q000592:standards*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics* / Q000378:metabolism / Q000592:standards*; D012334:RNA, Neoplasm / Q000235:genetics* / Q000378:metabolism; D012015:Reference Standards
D012334:RNA, Neoplasm; D012313:RNA