The epidemiological relationship between Salmonella enterica serovar typhimurium and Salmonella enterica serovar 4,[5],12:i:- isolates from humans and swine in Thailand.
A total of 138 isolates of S. Typhimurium and S. 4,[5],12:i:- from humans and swine in Thailand during 2003-2006, were evaluated for antimicrobial sensitivity by the disk diffusion method against 10 antimicrobial drugs and pulsed-field gel electrophoresis (PFGE) with endonuclease Xbal to investigate the epidemiological relationship among isolates. It was found that all isolates were classified into 27 antimicrobial resistance patterns, and 80% of S. Typhimurium and 95.4% of S. 4,[5],12:i:- isolates were resistant to three or more antimicrobial agents. By PFGE testing, the 84 PFGE patterns were categorized into A to Z patterns. Eighty percent of S. Typhimurium and 71.3% of S. 4,[5],12:i:- isolates in 7 major PFGE patterns had close clonal relationships (_85% similarity). Our studies indicate the spread of genetically identical clones of S. Typhimurium and S. 4,[5],12:i:- in humans and swine in Thailand.
WHO National Salmonella and Shigella Center, National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi, Thailand.
Srirat
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Pornruangwong
Thayat
T
Sriyapai
Chaiwat
C
Pulsrikarn
Pathom
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Sawanpanyalert
Sumalee
S
Boonmar
Aroon
A
Bangtrakulnonth
D000818:Animals; D004197:Disease Reservoirs / Q000382:microbiology* / Q000662:veterinary; D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D011159:Population Surveillance; D019779:Salmonella enterica / Q000187:drug effects* / Q000235:genetics; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics; D034421:Sus scrofa / Q000382:microbiology*; D013785:Thailand / Q000453:epidemiology