Class 1 integrons in multidrug-resistant non-typhoidal Salmonella enterica isolated in Spain between 2002 and 2004.
In this study, 119 multidrug-resistant isolates of non-typhoidal Salmonella enterica serovars collected in Spain (2002-2004) were screened for integrons. Among the isolates, 73.1% contained class 1 integrons, however classes 2 and 3 were not detected. Integrons containing gene cassettes were found in S. Enteritidis (16/32), S. Typhimurium biphasic (18/32) and monophasic [4,5,12:i:-] (11/19), S. Virchow (17/18) and S. Brandenburg (8/8), but not in S. Hadar (0/10). Ten complete and four incomplete gene cassettes, combined in 10 variable regions, were identified, one of which (2100 bp/dfrA1-597 bp-aadA24) was a new description. Most integrons mapped on plasmids of ca. 40-340 kb. Exceptions were 1000 bp/aadA2 and 1200 bp/bla PSE-1 found on the chromosome of biphasic S. Typhimurium, probably as part of SGI1-like structures.
Departamento de Biología Funcional, Area de Microbiología, Facultad de Medicina, Universidad de Oviedo, Julián Clavería n degrees 6, Oviedo, Spain.
Irene
I
Rodríguez
M Rosario
MR
Rodicio
Silvia
S
Herrera-León
Aurora
A
Echeita
M Carmen
MC
Mendoza
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D032023:Integrons / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D010957:Plasmids / Q000235:genetics; D015183:Restriction Mapping; D019779:Salmonella enterica / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012477:Salmonella enteritidis / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D012703:Serotyping; D013030:Spain
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; C025580:endodeoxyribonuclease XBAI; D015252:Deoxyribonucleases, Type II Site-Specific