Impaired lymphocyte responses and their restoration in oral cancer patients expressing distinct TCR variable region.
We have analyzed TCR Vbeta gene usage and clonality of T cells in the peripheral blood of patients with oral cancer and healthy individuals. A large repertoire of clonal TCR Vbeta was observed in the peripheral blood of cancer patients, and this clonal expansion was dominantly represented in the CD8+ T cells. A marked decrease in the lymphocyte proliferative responses to mitogen and anti-CD3 MAb and spontaneous apoptosis was observed in lymphocytes. Appropriate co-stimulation of lymphocytes (anti-TCR Vbeta MAb and anti-CD28 MAb) restored the lymphocyte proliferative responses and CD3-zeta chain expression in these patients.
Wadia
Persis
P
Chiplunkar lab, Immunology, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Navi Mumbai, India.
Rao
Dakshayini
D
Pradhan
Trupti
T
Pathak
Alok
A
Chiplunkar
Shubhada
S
D000328:Adult; D000911:Antibodies, Monoclonal; D017209:Apoptosis; D018106:CD28 Antigens / Q000097:blood; D017252:CD3 Complex / Q000097:blood; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D016022:Case-Control Studies; D049109:Cell Proliferation; D002478:Cells, Cultured; D002999:Clone Cells / Q000276:immunology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005838:Genotype; D006801:Humans; D008213:Lymphocyte Activation / Q000187:drug effects; D008297:Male; D008875:Middle Aged; D008934:Mitogens / Q000494:pharmacology; D009062:Mouth Neoplasms / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D010641:Phenotype; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000097:blood* / Q000235:genetics
D000911:Antibodies, Monoclonal; D018106:CD28 Antigens; D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D008934:Mitogens; D016693:Receptors, Antigen, T-Cell, alpha-beta