Alpha-hederin potentiates 5-FU antitumor activity in human colon adenocarcinoma cells.
The aim of this study was to investigate the ability of alpha-hederin to improve the efficacy of widely prescribed 5-fluorouracil (5-FU) in a human colon adenocarcinoma model. Drug combinations of alpha-hederin and 5-FU using both fixed-concentration and combination index methods were performed in vitro in HT-29 cells. The results showed that alpha-hederin at sub-IC(50) cytotoxic concentrations enhanced 5-FU cytotoxicity about 3.3-fold (p < 0.001). Simultaneous combination of alpha-hederin and 5-FU at their IC(50) ratio showed either a synergistic effect at a moderate cytotoxic range (25% of cell growth inhibition) or an antagonistic effect at a high level of growth inhibition. The data indicate therefore that it is possible to optimize colorectal cancer cell sensitivity to 5-FU with alpha-hederin.
Bun
Sok-Siya
SS
Laboratory of Pharmacognosy, UMR-D3, Faculty of Pharmacy, Marseille, France. siya.bun@pharmacie.univ-mrs.fr
Elias
Riad
R
Baghdikian
Béatrice
B
Ciccolini
Joseph
J
Ollivier
Evelyne
E
Balansard
Guy
G
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid; D004354:Drug Screening Assays, Antitumor; D005472:Fluorouracil / Q000494:pharmacology*; D019073:HT29 Cells; D006801:Humans; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D012503:Saponins / Q000494:pharmacology*
D000970:Antineoplastic Agents; D012503:Saponins; C010177:beta-hederin; D009828:Oleanolic Acid; D005472:Fluorouracil