Novel bone substitute composed of oligomeric proanthocyanidins-crosslinked gelatin and tricalcium phosphate.
A biodegradable GTP composite composed of OPCs crosslinked gelatin with tricalcium phosphate was prepared as a novel bone substitute. The effects of the degree of crosslinking on the swelling ratio, in vitro degradation rate, and morphology of the GTP composite are discussed. Adding OPCs to GP and GTP composites markedly reduced the degradation rate, indicating that OPCs are good crosslinking reagents for gelatin. Cytotoxic tests demonstrated that OPCs, gelatin and tricalcium phosphate released from GTP composite promoted the proliferation of MG-63 cells. Results of this study suggest that the nontoxic GTP composite is suitable for use as a large defect bone substitute.
Chen
Kuo-Yu
KY
Institute of Biomedical Engineering and Material Science, Central Taiwan University of Science and Technology, Taichung, Taiwan.
Shyu
Pei-Chi
PC
Chen
Yueh-Sheng
YS
Yao
Chun-Hsu
CH
D018786:Bone Substitutes / Q000138:chemical synthesis*; D002118:Calcium / Q000494:pharmacology; D002130:Calcium Phosphates / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002452:Cell Count; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D003432:Cross-Linking Reagents / Q000737:chemistry / Q000494:pharmacology*; D005780:Gelatin / Q000737:chemistry* / Q000494:pharmacology; D006801:Humans; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning; D044945:Proanthocyanidins / Q000737:chemistry* / Q000494:pharmacology; D012996:Solutions
D018786:Bone Substitutes; D002130:Calcium Phosphates; D003432:Cross-Linking Reagents; D044945:Proanthocyanidins; D012996:Solutions; D005780:Gelatin; C018392:tricalcium phosphate; D002118:Calcium