Glucuronide-sulfate diconjugate as a novel metabolite of glycyrrhetic acid in rat bile.
To study the metabolites of glycyrrhetic acid (GA) in rat bile, an analytical method was developed to identify GA and its metabolites by liquid chromatography mass spectrometry (LC-MS). Rat bile was collected after i.v. injection of GA. Three major GA-related peaks were detectable in rat bile by high-performance liquid chromatography (HPLC) analysis at 254 nm. LC-MS spectra showed their protonated molecular ions at m/z 727, 647, and 551. Furthermore, the three metabolites were also confirmed to exist in rat bile on LC-MS total ion chromatogram (TIC). Taken together with the susceptible nature to beta-glucuronidase digestion and alkaline conditions, they were identified to be a novel sulfate-glucuronide diconjugate and the known monoglucuronide and sulfate conjugate, respectively.
Center of Drug Metabolism & Pharmacokinetics, China Pharmaceutical University, Jiangsu, China.
Jing
J
Jing
Weichao
W
Ren
Xijing
X
Chen
Yue
Y
Wang
Qiaoling
Q
Yu
Guangji
G
Wang
Andrew K
AK
Davey
Jiping
J
Wang
Ning
N
Jing
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D001646:Bile / Q000378:metabolism*; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D020719:Glucuronides / Q000378:metabolism*; D006034:Glycyrrhetinic Acid / Q000008:administration & dosage / Q000378:metabolism / Q000493:pharmacokinetics*; D007275:Injections, Intravenous; D008297:Male; D015394:Molecular Structure; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D013431:Sulfates / Q000378:metabolism*
D000893:Anti-Inflammatory Agents; D020719:Glucuronides; D013431:Sulfates; D006034:Glycyrrhetinic Acid