Enzymatic hydrolysis of cellulose in aqueous two-phase systems. II. Semicontinuous conversion of a model substrate, solka floc BW 200.
A model substrate, Solka Floc BW 200, was semicontinuously hydrolyzed in an aqueous two-phase system based on crude dextran and polyethylene glycol over a period of more than 450 h. With an initial concentration of 75 g/L and intermittent addition of cellulose an average concentration of 50 g/L sugar was semicontinuously produced at dilution rates of 0.006-0.012 h(-1). The conversion of substrate varied between 49 and 66%. The enzyme consumption measured as FPU/g reducing sugar (RS) produced could be reduced by a factor two when compared to a batch process since, in the aqueous two phase system investigated, the enzyme could be recycled two times.
Department of Biochemistry, Chemical Center, University of Lund, P.O. Box 740, S-220 07 Lund, Sweden.
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Tjerneld
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Persson
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Albertsson
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Hahn-Hägerdal