Enantioseparation of kavain on Chiralpak IA under normal-phase, polar organic and reversed-phase conditions.
First baseline HPLC enantioseparation of kavain is described. Complete enantiodiscrimination was achieved on the immobilised-type Chiralpak IA chiral stationary phase (CSP) using pure methanol and simple methanol-water and ethanol-water mixtures as eluents. A water-dependent enantioselectivity was clearly demonstrated. Performance of the Chiralpak IA CSP in polar organic and RP conditions was compared with that of five coated polysaccharide-derived CSPs used in normal-phase mode.
Cirilli
Roberto
R
Dipartimento del Farmaco, Istituto Superiore di Sanità, Rome, Italy. rcirilli@iss.it <rcirilli@iss.it>
Ferretti
Rosella
R
Gallinella
Bruno
B
Bilia
Anna Rita
AR
Vincieri
Franco Francesco
FF
La Torre
Francesco
F
D000688:Amylose / Q000031:analogs & derivatives; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000431:Ethanol; D000432:Methanol; D011134:Polysaccharides / Q000737:chemistry; D011753:Pyrones / Q000737:chemistry / Q000302:isolation & purification*; D012997:Solvents; D013237:Stereoisomerism; D014867:Water
D011134:Polysaccharides; D011753:Pyrones; D012997:Solvents; D014867:Water; D000431:Ethanol; D000688:Amylose; C011889:kavain; D000432:Methanol