Self-organized functional lipid vesicle array for sensitive immunoassay chip.
We report the self-assembly immobilization of functional lipid vesicles (FLVs) by electrostatic interaction onto N-inscription-nanosized geometrics. The well-organized three-dimensional physical structures of liposome were observed by AFM. Generally, two involved forces for the binding to surfaces and the repulsion between individual liposome are necessary to array lipid vesicles individually similar to the physical configuration in solution. The immobilized FLVs demonstrated clearly defined redox activity in electrochemical measurements. We observed a notable current decrease, indicating the binding of the capture antibody with the target human serum albumin (HSA) antigen. We believe these findings can be related to various vesicles applications such as drug delivery system, nanobiosensors and nano-scale membrane function studies.
The Institute of Scientific and Industrial Research, Osaka University, Osaka 567-0047, Japan. hylee@sanken.osaka-u.ac.jp
Hea Yeon
HY
Lee
Bong Kuk
BK
Lee
Jong Wan
JW
Park
Ho Sup
HS
Jung
Jong Min
JM
Kim
Tomoji
T
Kawai
D000818:Animals; D015374:Biosensing Techniques; D002256:Carbonic Anhydrases / Q000276:immunology; D002417:Cattle; D004566:Electrodes; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D036103:Nanotechnology; D009419:Nerve Tissue Proteins / Q000737:chemistry; D040081:Protein Array Analysis / Q000379:methods*; D012709:Serum Albumin / Q000276:immunology; D055672:Static Electricity; D019809:Streptavidin / Q000737:chemistry
C029152:G-substrate; D008055:Lipids; D008081:Liposomes; D009419:Nerve Tissue Proteins; D012709:Serum Albumin; D019809:Streptavidin; D002256:Carbonic Anhydrases