Isolation and characterization of mesenchymal stem cell population entrapped in bone marrow collection sets.
Bone marrow is an important source of mesenchymal stem cells (MSCs), and a promising tool for cytotherapy. MSC utilization is limited by low cell yields obtained under standard isolation protocols. Herein, used bone marrow collection sets were evaluated as a valuable source of MSCs. Adherent cells washed from the collection sets were examined for widely accepted criteria defining MSCs. Significant numbers of cells (median 9million per set in passage 1) with colony-forming activity and high proliferative potential at low seeding densities were obtained. These cells were positive for essential MSC surface molecules (CD90, CD105, CD166, CD44, CD29) and negative for most haematopoietic and endothelial cell markers (CD45, CD34, CD11a, CD235a, HLA-DR, CD144). The cells were capable of differentiation along adipogenic, osteogenic and chondrogenic pathways. Washing out bone marrow collection sets may constitute a highly ethical source of MSCs for research purposes and may be utilized also in clinical applications.
Laboratory of Molecular Biology, CPN spol.s.r.o., Dolní Dobrouc 401, CZ561 02 Dolní Dobrouc, Czech Republic. dvorakova@contipro.cz
Jana
J
Dvorakova
Alena
A
Hruba
Vladimir
V
Velebny
Lukas
L
Kubala
D050156:Adipogenesis; D000911:Antibodies, Monoclonal; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D002452:Cell Count; D018929:Cell Culture Techniques / Q000379:methods*; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D048430:Cell Shape; D020219:Chondrogenesis; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D010012:Osteogenesis
D000911:Antibodies, Monoclonal; D015415:Biomarkers