Enzyme immobilization in porous solid supports-penetration of immobilized enzyme.
The process of enzyme immobilization under the diffusion-controlled regime (i.e., fast attachment of enzyme compared to its diffusion) is modeled and theoretically solved in this article. Simple and compact solutions for the penetration depth of immobilized enzyme and the bulk enzyme concentration versus time are presented. Furthermore, the conditions for the validity of our solutions are also given in this article so that researchers can discover when the theoretical solutions can be applied to their systems.
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Department of Chemical Engineering, University of Queensland, St. Lucia, Queensland 4067, Australia.