Efficacy of antioxidants in the yeast Saccharomyces cerevisiae correlates with their effects on protein thiols.
We have found previously that only a limited number of antioxidants are able to protect yeast cells against endogenous and exogenous oxidative stress. In search of factors determining this selectivity of antioxidant action we compared the ability of a set of antioxidants to: (i) protect a thiol-dependent enzyme alcohol dehydrogenase (ADH) against inactivation by superoxide, peroxynitrite and hydrogen peroxide; (ii) prevent H(2)O(2)-induced activation of Yap1 p; and (iii) decrease extracellular redox potential of the medium. The results obtained provide demonstration with respect to yeast that the ability to lower redox potential and to maintain critical thiol groups in the reduced state is an important facet of the action of antioxidants.
Department of Biochemistry and Cell Biology, University of Rzeszow, Rzeszow, Poland. skoziol@univ.rzeszow.pl
Sabina
S
Bednarska
Pierre
P
Leroy
Marek
M
Zagulski
Grzegorz
G
Bartosz
D000426:Alcohol Dehydrogenase / Q000378:metabolism; D000975:Antioxidants / Q000494:pharmacology*; D003470:Culture Media; D004789:Enzyme Activation / Q000187:drug effects; D005110:Extracellular Space / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000494:pharmacology; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress; D030421:Peroxynitrous Acid / Q000494:pharmacology; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000378:metabolism*; D013481:Superoxides / Q000494:pharmacology; D014157:Transcription Factors / Q000378:metabolism
D000975:Antioxidants; D003470:Culture Media; D029701:Saccharomyces cerevisiae Proteins; D013438:Sulfhydryl Compounds; D014157:Transcription Factors; C086677:YAP1 protein, S cerevisiae; D013481:Superoxides; D030421:Peroxynitrous Acid; D006861:Hydrogen Peroxide; D000426:Alcohol Dehydrogenase