hBolA, novel non-classical secreted proteins, belonging to different BolA family with functional divergence.
This study reported that all three human BolA proteins (hBolA1, hBolA2, and hBolA3) are novel non-classical secreted proteins identified with bioinformatics and molecular biology experiments. The three BolA fusion proteins with c-Myc tag could be secreted into the culture medium of the transfected Cos-7 cells, although they could not be colocalized with Golgi apparatus. And the secretion of three BolA proteins could not be inhibited after BFA treatment. Furthermore, the secretion was not dependent on its predicted signal peptide. All the experiment results suggested that the secretion was a non-classical export. Phylogenetic analysis showed that the human BolAs belong to three different groups with functional divergence of BolA subfamily, where the different helix-turn-helix motif among hBolA1, hBolA2, and hBolA3 could be responsible for their functional divergence. Our data provided a basis for functional studies of BolA protein family.
Zhou
Yu-Bo
YB
Chinese National Human Genome Center at Shanghai, 351 Guo Shou-Jing Road, Shanghai, 201203, China.
Cao
Jia-Bing
JB
Wan
Bing-Bing
BB
Wang
Xin-Rong
XR
Ding
Guo-Hui
GH
Zhu
Hong
H
Yang
Hong-Meng
HM
Wang
Ke-Sheng
KS
Zhang
Xin
X
Han
Ze-Guang
ZG
D000595:Amino Acid Sequence; D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D019295:Computational Biology; D005057:Eukaryotic Cells / Q000378:metabolism; D019143:Evolution, Molecular; D014644:Genetic Variation; D006801:Humans; D024101:Mitochondrial Proteins; D008969:Molecular Sequence Data; D050505:Mutant Proteins / Q000378:metabolism; D010802:Phylogeny; D021382:Protein Sorting Signals; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry / Q000378:metabolism*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013347:Subcellular Fractions / Q000378:metabolism
C533595:BOLA1 protein, human; C533596:BOLA2 protein, human; C533597:BolA3 protein, human; D024101:Mitochondrial Proteins; D050505:Mutant Proteins; D021382:Protein Sorting Signals; D011506:Proteins; D011993:Recombinant Fusion Proteins