Low cytotoxicity of creams and lotions formulated with Buriti oil (Mauritia flexuosa) assessed by the neutral red release test.
The aim of this work was to evaluate possible cytotoxic effects of topical creams and lotions produced with Buriti oil and commercial surfactants on human keratinocytes HaCat and 3T3 embryonic mouse fibroblast cultures. We also aimed to assess the cytotoxicity of the surfactants used to produce the emulsions. The neutral red release (NRR) assay was performed as an in vitro method to evaluate the cytotoxicity of the emulsions in HaCat and 3T3 cell lines and predict potential skin irritation. The Buriti oil emulsions presented low cytotoxicity to the cells at high concentrations and the addition of Vitamin E increased cell viability. Among the surfactant tested, Unitol CE 200F proved to be the most cytotoxic, presenting an IC(50) significantly lower than the others. Emulsions formulated with Buriti oil and commercial surfactants could be non irritant to the skin due to their low cytotoxicity, especially when enhanced with vitamin E. When emulsified with Buriti oil, water and Brij 72, Unitol CE 200F showed less cytotoxic effects than when tested alone.
Departament de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Brazil.
Cinthia Fernanda
CF
Zanatta
Vanessa
V
Ugartondo
Montserrat
M
Mitjans
Pedro A
PA
Rocha-Filho
María Pilar
MP
Vinardell
D016475:3T3 Cells; D000818:Animals; D028023:Arecaceae / Q000737:chemistry*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D002626:Chemistry, Pharmaceutical; D004396:Coloring Agents; D004655:Emulsions; D006801:Humans; D015603:Keratinocytes / Q000187:drug effects; D051379:Mice; D009499:Neutral Red; D009824:Ointments; D000073878:Palm Oil; D010938:Plant Oils / Q000008:administration & dosage / Q000633:toxicity*; D013501:Surface-Active Agents / Q000008:administration & dosage / Q000633:toxicity*; D014810:Vitamin E / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology
D004396:Coloring Agents; D004655:Emulsions; D009824:Ointments; D010938:Plant Oils; D013501:Surface-Active Agents; D014815:Vitamins; D014810:Vitamin E; D009499:Neutral Red; D000073878:Palm Oil