[Effect of pinggan qianyang on hypothalamic proteome in the hyperthyroid rats with hyperactivity of liver-yang].
OBJECTIVE
To investigate the effect of pinggan-qianyang (PGQY), a Chinese medicine, on hypothalamic proteome in the hyperthyroid rats with hyperactivity of liver-yang, and to explore its mechanism.


METHODS
The rat model was established by intraperitoneal injection of levo-thyroxine (L-T4) and fuzi decotion. All the quantitative and qualitative changes of the protein expressions were compared among the normal group,the model group and the treatment group by proteomic techniques.


RESULTS
The protein spots in the 3 groups were mainly displayed at the isoelectric point (pI) 3 approximately 10, and the molecular weights were 13.8 approximately 98.8 kD.Compared with the normal group, 6 spots of protein expression increased and 10 decreased in the model group. All the changed protein in the model group returned to normal level after PGQY treatment. Mass-spectrometer and bio-informatics indicated that these proteins were Prohibitin, Peroxiredoxin-6, histidine triad nucleotide-binding protein 1, protein-tyrosine-phosphatase, predicted protein, profilin-2, peroxir doxin-II, heat shock protein-27, and annexin-A1.


CONCLUSIONS
There are differences in the expression of hypothalamus proteins in the hyperthyroid rats with hyperactivity of liver-yang after the treatment with PGQY, and the 9 identified protein spots may be associated with the mechanism of PGQY.
Institute of Combined Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, China.
Yao-hui
YH
Yin
Zhen-jia
ZJ
Yi
Guang-wei
GW
Zhong
Jian-jun
JJ
Hu
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use; D005260:Female; D006980:Hyperthyroidism / Q000188:drug therapy* / Q000378:metabolism*; D007031:Hypothalamus / Q000378:metabolism*; D008297:Male; D008516:Medicine, Chinese Traditional; D009419:Nerve Tissue Proteins / Q000378:metabolism; D054465:Peroxiredoxin VI / Q000378:metabolism; D008517:Phytotherapy; D000091402:Prohibitins; D020543:Proteome / Q000378:metabolism*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012097:Repressor Proteins / Q000378:metabolism
D004365:Drugs, Chinese Herbal; C063306:HINT1 protein, human; D009419:Nerve Tissue Proteins; D000091402:Prohibitins; D020543:Proteome; D012097:Repressor Proteins; D054465:Peroxiredoxin VI