Optimization of octanoic acid and sulfur donor concentrations for lipoic acid production by Pseudomonas reptilivora.
Using the optimal concentrations of octanoic acid (0.75 mM) and ethyl mercaptan (2 mM), as the most effective sulfur donor, Pseudomonas reptilivora produced 74 microg lipoic acid per dry cell weight at pH 7.5 and 30 degrees C in a fermenter over 9 h. The dry cell weight was 13.9 g l(-1).
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, South Korea.
Jung-Hwan
JH
Ji
In-Sik
IS
Yu
Hye-Jung
HJ
Kim
Deok-Kun
DK
Oh
D002210:Caprylates / Q000378:metabolism*; D005285:Fermentation; D011549:Pseudomonas / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D008063:Thioctic Acid / Q000096:biosynthesis*
D002210:Caprylates; D013455:Sulfur; D008063:Thioctic Acid; C031492:octanoic acid