Identification of diverse carbon utilization pathways in Shewanella oneidensis MR-1 via expression profiling.
To identify pathways of carbon utilization in the metal-reducing marine bacterium Shewanella oneidensis MR-1, we assayed the expression of cells grown with various carbon sources using a high-density oligonucleotide Affymetrix microarray. Our expression profiles reveal genes and regulatory mechanisms which govern the sensing, import, and utilization of the nucleoside inosine, the chitin monomer N-acetylglucosamine, and a casein-derived mixture of amino acids. Our analysis suggests a prominent role for the pentose-phosphate and Entner-Doudoroff pathways in energy metabolism, and regulatory coupling between carbon catabolism and electron acceptor pathways. In sum, these results indicate that S. oneidensis possesses a broader capacity for carbon utilization than previously reported, a view with implications for optimizing its role in microbial fuel cell and bioremediative applications.
Driscoll
Michael E
ME
Boston University, St. Boston, MA 02215, USA. mdriscol@bu.edu
Romine
Margie F
MF
Juhn
Frank S
FS
Serres
Margrethe H
MH
McCue
Lee Anne
LA
Beliaev
Alex S
AS
Fredrickson
James K
JK
Gardner
Timothy S
TS
D000117:Acetylglucosamine / Q000378:metabolism; D002244:Carbon / Q000378:metabolism*; D002686:Chitin / Q000378:metabolism; D020869:Gene Expression Profiling; D007288:Inosine / Q000378:metabolism; D010427:Pentose Phosphate Pathway; D020592:Shewanella / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
D002686:Chitin; D007288:Inosine; D002244:Carbon; D000117:Acetylglucosamine