Growth of animal cells on microbeads. I. In situ estimation of numbers.
Evaluation of the number of anchorage-dependent cells growing on the surface of microbeads kept in suspension in a bioreactor is a tedious procedure fraught with many difficulties. Using the Coulter counter as a biomass probe, this article shows that the number of cells adhering to microbeads can be determined while the cells are still attached.
Miller
S J
SJ
Laboratory of Molecular Biochemistry, Faculty of Medicine, Mons State University, Avenue du Champ de Mars, 24-7000, Mons, Belgium.
Henrotte
M
M
Miller
A O
AO