Reliability of different image analysis methods for scanning laser Doppler flowmetry.
OBJECTIVE
To assess reliability and reproducibility of different analysis methods for retinal capillary flow, volume, and velocity from scanning laser Doppler flowmetry (SLDF) topography.


METHODS
SLDF topography analysis using the default retinal flowmeter (HRF) were compared to that using automatic full-field perfusion image analyzer (AFFPIA) and quantified as intraclass correlation coefficients (ICC).


RESULTS
The AFFPIA full-field method had the highest reliability, with ICC 0.99 for capillary flow. The reproducibility using the AFFPIA full-field method was high ICC 0.74 for capillary flow.


CONCLUSIONS
The AFFPIA full-field method is highly reliable and superior to the default HRF software.
Centre for Eye Research Australia, University of Melbourne, Australia.
Andreas J
AJ
Kreis
Thanh
T
Nguyen
Sophie
S
Rogers
Jie Jin
JJ
Wang
Joanna
J
Harazny
Georg
G
Michelson
H M Omar
HM
Farouque
Tien Y
TY
Wong
D000328:Adult; D001783:Blood Flow Velocity / Q000502:physiology; D001810:Blood Volume / Q000502:physiology; D002196:Capillaries / Q000502:physiology; D003430:Cross-Sectional Studies; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D017078:Laser-Doppler Flowmetry / Q000592:standards*; D008297:Male; D008875:Middle Aged; D015588:Observer Variation; D015203:Reproducibility of Results; D012171:Retinal Vessels / Q000502:physiology*