Uptake and depuration of anatoxin-a by the mussel Mytilus galloprovincialis (Lamarck, 1819) under laboratory conditions.
Cyanobacterial blooms tend to be more common in warm and nutrient-enriched waters and are increasing in many aquatic water bodies due to eutrophication. The aim of this work is to study the accumulation and depuration of anatoxin-a by Mytilus galloprovincialis a widespread distributed mussel living in estuarine and coastal waters and recognized worldwide as a bioindicator (e.g. Mussel Watch programs). Research on the distribution and biological effects of anatoxin-a in M. galloprovincialis is important. Nevertheless, the risk of human intoxication due to the consumption of contaminated bivalves should also be considered. A toxic bloom was simulated in an aquarium with 5 x 10(5) cell ml(-1) of Anabaena sp. (ANA 37), an anatoxin-a producing strain. Mussels were exposed to Anabaena for 15 days and then 15 days of depuration followed. Three or more animals were sampled every 24h for total toxin quantification and distribution in soft tissues (edible parts). Water samples were also taken every 24h in order to calculate total dissolved and particulate anatoxin-a concentrations. Anatoxin-a was quantified by HPLC with fluorescence detection. No deaths occurred during accumulation and depuration periods. One day after the beginning of depuration, the toxin could not be detected in the animals. Anatoxin-a is distributed in the digestive tract, muscles and foot and is probably actively detoxified.
Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas, 289, Porto, Portugal.
Joana
J
Osswald
Sandra
S
Rellán
Ana
A
Gago
Vitor
V
Vasconcelos
D017033:Anabaena / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal / Q000502:physiology; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid; D000087522:Cyanobacteria Toxins; D005612:Freeze Drying; D005618:Fresh Water / Q000382:microbiology; D049878:Mytilus / Q000378:metabolism*; D012015:Reference Standards; D014326:Tropanes / Q000378:metabolism*; D014867:Water / Q000737:chemistry
D000087522:Cyanobacteria Toxins; D014326:Tropanes; D014867:Water; C509783:anatoxin a