Immune killing activity of lymphocytes on Hela cells expressing interleukin-12 in vitro.
The killing effects of lymphocytes on Hela cells expressing interleukin-12 (IL-12) in vitro were explored. By using gene transfection technique, full length IL-12 gene was transfected into Hela cells. The expression of IL-12 in Hela cells was detected quantitatively by ELISA; Changes in killing effects of lymphocytes on Hela cells expressing IL-12 were observed by MTT. It was found that Hela cells could express IL-12 between 24 h and 72 h after transfection. Killing activity of lymphocytes on Hela cells expressing IL-12 was significantly enhanced. It was concluded by cell transfection technique, Hela cells could express IL-12 and were more easily killed by lymphocytes.
Hospital of Wuhan University of Technology, Wuhan 430070, China. huiyanw@whut.edu.cn
Huiyan
H
Wang
Suhua
S
Chen
D017209:Apoptosis; D002470:Cell Survival; D018920:Coculture Techniques; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D007107:Immune System; D018664:Interleukin-12 / Q000096:biosynthesis* / Q000378:metabolism / Q000502:physiology; D007694:Killer Cells, Natural / Q000166:cytology; D008214:Lymphocytes / Q000378:metabolism* / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology; D013778:Tetrazolium Salts / Q000494:pharmacology; D013844:Thiazoles / Q000494:pharmacology; D013997:Time Factors; D002583:Uterine Cervical Neoplasms / Q000378:metabolism*
D013778:Tetrazolium Salts; D013844:Thiazoles; D018664:Interleukin-12; C022616:thiazolyl blue