Escherichia coli colonizing the neurogenic bladder are similar to widespread clones causing disease in patients with normal bladder function.
METHODS
Clonal typing of neurogenic clones.


OBJECTIVE
To determine whether neurogenic clones carried over weeks in the urine of asymptomatic children with neurogenic bladder were similar to known uropathogenic clones associated with disease.


METHODS
Michigan State University; VA Medical Center, Minneapolis, MN, USA.


METHODS
Escherichia coli isolates from the urine of 15 children previously followed were typed by multilocus sequence typing and compared to 2 human pyelonephritis genome strains, 29 pediatric or adult symptomatic urinary tract infection strains, 15 pediatric asymptomatic bacteriuria strains, 6 animal urinary tract infection strains and a neonatal meningitis-septicemia prototype K1 strain. Phylotypes and virulence genotypes were determined using PCR.


RESULTS
Twenty-nine E. coli isolates were classified into 15 clones. Six of 15 clones were the same sequence type or a close relative to a clone that caused disease in a human or animal. These clones were considered uropathogens. The remaining nine clones were not closely related to a clone that caused disease and were considered commensal clones. Uropathogens were predominantly group B2, exhibited more virulence genes and were carried for more weeks in the neurogenic bladder compared to commensal clones. Nine of 15 children carried one or more uropathogenic clones; 4 children carried one or more commensal clones and 2 children carried both uropathogenic and commensal clones.


CONCLUSIONS
Children with neurogenic bladder most commonly carried commensal clones. Uropathogenic clones were associated with prolonged carriage, however, carriage was not associated with symptomatic disease or deterioration of the upper urinary tract.
University of Virginia, Charlottesville, VA 22908-0699, USA. tas8n@virginia.edu
T A
TA
Schlager
J R
JR
Johnson
L M
LM
Ouellette
T S
TS
Whittam
D000818:Animals; D001422:Bacterial Adhesion / Q000235:genetics; D001437:Bacteriuria / Q000175:diagnosis / Q000382:microbiology*; D001483:Base Sequence / Q000235:genetics; D002648:Child; D002999:Clone Cells; D015169:Colony Count, Microbial; D003556:Cystitis / Q000382:microbiology; D004926:Escherichia coli / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000145:classification* / Q000175:diagnosis; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D010802:Phylogeny; D001750:Urinary Bladder, Neurogenic / Q000150:complications*; D014552:Urinary Tract Infections / Q000175:diagnosis / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics
D037521:Virulence Factors