An international standardization programme towards the application of gene expression profiling in routine leukaemia diagnostics: the Microarray Innovations in LEukemia study prephase.
Gene expression profiling has the potential to enhance current methods for the diagnosis of haematological malignancies. Here, we present data on 204 analyses from an international standardization programme that was conducted in 11 laboratories as a prephase to the Microarray Innovations in LEukemia (MILE) study. Each laboratory prepared two cell line samples, together with three replicate leukaemia patient lysates in two distinct stages: (i) a 5-d course of protocol training, and (ii) independent proficiency testing. Unsupervised, supervised, and r(2) correlation analyses demonstrated that microarray analysis can be performed with remarkably high intra-laboratory reproducibility and with comparable quality and reliability.
Kohlmann
Alexander
A
Roche Molecular Systems, Inc., Department of Genomics and Oncology, Pleasanton, CA, USA. alexander.kohlmann@roche.com
Kipps
Thomas J
TJ
Rassenti
Laura Z
LZ
Downing
James R
JR
Shurtleff
Sheila A
SA
Mills
Ken I
KI
Gilkes
Amanda F
AF
Hofmann
Wolf-Karsten
WK
Basso
Giuseppe
G
Dell'orto
Marta Campo
MC
Foà
Robin
R
Chiaretti
Sabina
S
De Vos
John
J
Rauhut
Sonja
S
Papenhausen
Peter R
PR
Hernández
Jesus M
JM
Lumbreras
Eva
E
Yeoh
Allen E
AE
Koay
Evelyn S
ES
Li
Rachel
R
Liu
Wei-Min
WM
Williams
Paul M
PM
Wieczorek
Lothar
L
Haferlach
Torsten
T
D000230:Adenocarcinoma / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics; D002277:Carcinoma / Q000235:genetics; D005060:Europe; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods / Q000592:standards*; D006801:Humans; D007938:Leukemia / Q000175:diagnosis / Q000235:genetics*; D008113:Liver Neoplasms / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods / Q000592:standards*; D010865:Pilot Projects; D012313:RNA; D012015:Reference Standards; D015203:Reproducibility of Results; D012846:Singapore; D014481:United States
D012313:RNA