Heme oxygenase-1 attenuates contact hypersensitivity induced by 2,4-dinitrofluorobenzene in mice.
Heme oxygenase (HO)-1 may have an important role in the resolution of T cell-mediated inflammation. The authors elucidated the role of the anti-inflammatory HO-1 in the pathogenesis of skin inflammation, using a mouse contact hypersensitivity (CHS) induced by 2,4-dinitrofluorobenzene (DNFB). Ear swelling was induced by challenge with DNFB, accompanied by infiltration of inflammatory cells in the challenged ear skin. DNFB challenge induced low levels of HO-1 mRNA and protein expression. Ear swelling induced by DNFB challenge was significantly reduced by topical treatment with cobalt protoporphyrin IX (CoPP), a HO-1 inducer, but exaggerated by blockage of HO-1 activity with tin protoporphyrin IX (SnPP), a HO-1 inhibitor. Similarly, the number of infiltrated cells in DNFB-challenged ear skin were reduced by CoPP but increased by SnPP. Our findings suggest that HO-1 plays an important role in CHS and is an important pharmacological target for the treatment of CHS.
Pae
Hyun-Ock
HO
Department of Microbiology and Immunology, Wonkwang University School of Medicine, Iksan, Republic of Korea.
Ae Ha
Young
Y
Chai
Kyu-Yun
KY
Chung
Hun-Taeg
HT
D000818:Animals; D003877:Dermatitis, Contact / Q000201:enzymology* / Q000209:etiology / Q000473:pathology; D004139:Dinitrofluorobenzene / Q000633:toxicity*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D051547:Heme Oxygenase-1 / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D007249:Inflammation / Q000139:chemically induced / Q000201:enzymology / Q000473:pathology; D008565:Membrane Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D051379:Mice; D017319:Photosensitizing Agents / Q000494:pharmacology; D011524:Protoporphyrins / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D012867:Skin / Q000201:enzymology* / Q000473:pathology
D008565:Membrane Proteins; D017319:Photosensitizing Agents; D011524:Protoporphyrins; D012333:RNA, Messenger; C028025:protoporphyrin IX; D004139:Dinitrofluorobenzene; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse