Resonance light-scattering enhancement effect of the protein-Y3+-TTA-SLS system and its analytical application.
In this paper, a sensitive resonance light scattering (RLS) method for the determination of protein is reported. In the Tris-HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y(3+)-2-thenoyltrifluoroacetone (TTA)-sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 x 10(-9)-1.0 x 10(-5) g/mL for BSA, 1.0 x 10(-8)-1.0 x 10(-5) g/mL for HSA and 1.0 x 10(-8)-1.0 x 10(-6 )g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied.
Guo
Changying
C
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, People's Republic of China.
Wu
Xia
X
Xu
Wei
W
Yang
Jinghe
J
D002021:Buffers; D002614:Chelating Agents / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008027:Light; D010047:Ovalbumin / Q000032:analysis; D011506:Proteins / Q000032:analysis*; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012709:Serum Albumin / Q000032:analysis; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry*; D013501:Surface-Active Agents / Q000737:chemistry*; D013804:Thenoyltrifluoroacetone / Q000737:chemistry*; D015019:Yttrium / Q000737:chemistry*
D002021:Buffers; D002614:Chelating Agents; D011506:Proteins; D012709:Serum Albumin; D013501:Surface-Active Agents; D013804:Thenoyltrifluoroacetone; D012967:Sodium Dodecyl Sulfate; D015019:Yttrium; D010047:Ovalbumin; C091417:yttria