Peptide release on the ribosome depends critically on the 2' OH of the peptidyl-tRNA substrate.
Peptide release on the ribosome is catalyzed by protein release factors (RFs) on recognition of stop codons positioned in the A site of the small ribosomal subunit. Here we show that the 2' OH of the peptidyl-tRNA substrate plays an essential role in catalysis of the peptide release reaction. These observations parallel earlier studies of the mechanism of the peptidyl transfer reaction and argue that related mechanisms are at the heart of catalysis for these reactions.
Brunelle
Julie L
JL
Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Shaw
Jeffrey J
JJ
Youngman
Elaine M
EM
Green
Rachel
R
D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D010443:Peptide Chain Termination, Translational; D010454:Peptide Termination Factors / Q000378:metabolism; D010455:Peptides / Q000378:metabolism*; D014176:Protein Biosynthesis; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry / Q000378:metabolism*; D012270:Ribosomes / Q000378:metabolism*
D010454:Peptide Termination Factors; D010455:Peptides; D012346:RNA, Transfer, Amino Acyl; C019037:tRNA, peptidyl-