A femtosecond laser pacemaker for heart muscle cells.
The intracellular effects of focused near-infrared femtosecond laser irradiation are shown to cause contraction in cultured neonatal rat cardiomyocytes. By periodic exposure to femtosecond laser pulse-trains, periodic contraction cycles in cardiomyocytes could be triggered, depleted, and synchronized with the laser periodicity. This was observed in isolated cells, and in small groups of cardiomyocytes with the laser acting as pacemaker for the entire group. A window for this effect was found to occur between 15 and 30 mW average power for an 80 fs, 82 MHz pulse train of 780 nm, using 8 ms exposures applied periodically at 1 to 2 Hz. At power levels below this power window, laser-induced cardiomyocyte contraction was not observed, while above this power window, cells typically responded by a high calcium elevation and contracted without subsequent relaxation. This laser-cell interaction allows the laser irradiation to act as a pacemaker, and can be used to trigger contraction in dormant cells as well as synchronize or destabilize contraction in spontaneously contracting cardiomyocytes. By increasing laser power above the window available for laser-cell synchronization, we also demonstrate the use of cardiomyocytes as optically-triggered actuators. To our knowledge, this is the first demonstration of remote optical control of cardiomyocytes without requiring exogenous photosensitive compounds.
Department of Frontier Biosciences, Graduate School of Frontier Biosciences Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. nsmith@ap.eng.osaka-u.ac.jp
N I
NI
Smith
Y
Y
Kumamoto
S
S
Iwanaga
J
J
Ando
K
K
Fujita
S
S
Kawata
D000200:Action Potentials / Q000502:physiology* / Q000528:radiation effects; D000818:Animals; D000831:Animals, Newborn; D002304:Cardiac Pacing, Artificial / Q000379:methods*; D002478:Cells, Cultured; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007834:Lasers; D009200:Myocardial Contraction / Q000502:physiology* / Q000528:radiation effects; D032383:Myocytes, Cardiac / Q000502:physiology* / Q000528:radiation effects; D010138:Pacemaker, Artificial; D051381:Rats; D017208:Rats, Wistar