Regulation of the transcription factor Ets-1 by DNA-mediated homo-dimerization.
The function of the Ets-1 transcription factor is regulated by two regions that flank its DNA-binding domain. A previously established mechanism for auto-inhibition of monomeric Ets-1 on DNA response elements with a single ETS-binding site, however, has not been observed for the stromelysin-1 promoter containing two palindromic ETS-binding sites. We present the structure of Ets-1 on this promoter element, revealing a ternary complex in which protein homo-dimerization is mediated by the specific arrangement of the two ETS-binding sites. In this complex, the N-terminal-flanking region is required for ternary protein-DNA assembly. Ets-1 variants, in which residues from this region are mutated, loose the ability for DNA-mediated dimerization and stromelysin-1 promoter transactivation. Thus, our data unravel the molecular basis for relief of auto-inhibition and the ability of Ets-1 to function as a facultative dimeric transcription factor on this site. Our findings may also explain previous data of Ets-1 function in the context of heterologous transcription factors, thus providing a molecular model that could also be valid for Ets-1 regulation by hetero-oligomeric assembly.
Lamber
Ekaterina P
EP
EMBL-Hamburg, c/o DESY, Hamburg, Germany.
Vanhille
Laurent
L
Textor
Larissa C
LC
Kachalova
Galina S
GS
Sieweke
Michael H
MH
Wilmanns
Matthias
M
D002460:Cell Line; D018360:Crystallography, X-Ray; D004247:DNA / Q000378:metabolism*; D019281:Dimerization; D019278:Matrix Metalloproteinase 3 / Q000235:genetics*; D008958:Models, Molecular; D011401:Promoter Regions, Genetic; D050784:Proto-Oncogene Protein c-ets-1 / Q000737:chemistry* / Q000378:metabolism*; D050436:Regulatory Elements, Transcriptional; D015533:Transcriptional Activation
C494041:ETS1 protein, human; D050784:Proto-Oncogene Protein c-ets-1; D004247:DNA; C511768:MMP3 protein, human; D019278:Matrix Metalloproteinase 3