Exposure of Vicia faba and Pisum sativum to copper-induced genotoxicity.
The potential genotoxicity of Cu(2+) was investigated in Vicia faba and Pisum sativum seedlings in hydroponic culture conditions. Cu(2+) caused a dose-dependent increase in micronuclei frequencies in both plant models. Cytological analysis of root tips cells showed clastogenic and aneugenic effects of this heavy metal on V. faba root meristems. Cu(2+) induced chromosomal alterations at the lowest concentration used (2.5 mM) when incubated for 42 h, indicating the potent mutagenic effect of this ion. A spectrum of chromosomal abnormalities was observed in V. faba root meristems, illustrating the genotoxic events leading to micronuclei formation.
Laboratoire de Physiologie et Génétique Végétales, EA ERTAC, Université Blaise Pascal-Clermont II, Campus Universitaire des Cézeaux, Aubière, France.
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Souguir
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Ferjani
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Ledoigt
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Goupil
D032461:Chromosomes, Plant / Q000187:drug effects; D003300:Copper / Q000494:pharmacology*; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D004785:Environmental Pollutants / Q000494:pharmacology*; D018519:Meristem / Q000187:drug effects; D015162:Micronucleus Tests; D018532:Pisum sativum / Q000187:drug effects* / Q000235:genetics; D018517:Plant Roots / Q000187:drug effects; D031307:Vicia faba / Q000187:drug effects* / Q000235:genetics
D004785:Environmental Pollutants; D003300:Copper