Diminished mitochondrial DNA integrity and repair capacity in OA chondrocytes.
OBJECTIVE
Osteoarthritis (OA) is characterized by the failure of chondrocytes to respond to injury and perform the cartilage remodeling process. Human articular chondrocytes actively produce reactive oxygen and nitrogen species (ROS and RNS) capable of causing cellular dysfunction and death. A growing body of evidence indicates that mitochondrial dysfunction and mitochondrial DNA (mtDNA) damage play a causal role in disorders linked to excessive generation of oxygen free radicals. The aim of this study was to determine whether mtDNA damage was present in OA chondrocytes, and whether mtDNA repair capacity is compromised in OA chondrocytes following oxidative stress, leading to chondrocyte death.


METHODS
Human articular cartilage was isolated from knee joints of cadavers available through the Anatomical Gifts Program at the University of South Alabama (normal donors) or OA patients undergoing total knee replacement surgeries (OA patients). Total DNA was isolated from either chondrocytes released following collagenase digestion, or from first passage chondrocytes grown in culture and exposed to ROS or RNS. mtDNA integrity and repair capacity were analyzed by quantitative Southern blot analysis, using a mtDNA-specific radioactive probe. Cell viability was determined by the trypan blue exclusion method.


RESULTS
mtDNA damage was found in chondrocytes from OA patients compared to normal donors. It was accompanied with reduced mtDNA repair capacity, cell viability, and increased apoptosis in OA chondrocytes following exposure to ROS and RNS.


CONCLUSIONS
These results indicate that mtDNA damage and poor mtDNA repair capacity for removing damage caused by oxidative stress may contribute to the pathogenesis of OA.
Department of Cell Biology, University of South Alabama, Mobile, AL 36688-0002, USA. vgrishko@jaguar1.usouthal.edu
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Grishko
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Wilson
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Pearsall
D000368:Aged; D017209:Apoptosis / Q000187:drug effects; D002358:Cartilage, Articular / Q000187:drug effects / Q000473:pathology*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology*; D004260:DNA Repair; D004272:DNA, Mitochondrial / Q000235:genetics*; D006801:Humans; D008875:Middle Aged; D020370:Osteoarthritis, Knee / Q000235:genetics* / Q000473:pathology; D018384:Oxidative Stress / Q000235:genetics; D026361:Reactive Nitrogen Species / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000494:pharmacology
D004272:DNA, Mitochondrial; D026361:Reactive Nitrogen Species; D017382:Reactive Oxygen Species