[The change of proliferative potential of fibroblasts derived of nasal polyps in vitro cultured, influenced by derivatives vitamins D].
BACKGROUND
Recurrent polyposis in the same patient resulting in the necessity of repeated surgeries forced to search for new pharmacological therapeutic methods. At present, locally acting glycocorticosteroids have the greatest value in the treatment of nasal polyposis. Polyps grow is connected with inflammation process and proliferation of fibroblasts.


OBJECTIVE
An evaluation of calcitriol and tacalcitol influence on proliferation of fibroblasts extracted from nasal polyps.


METHODS
consisted of 9 tissue samples coming from nasal polyps sampled during polypectomies. The testing was performed on the polyps fibroblasts after the sixth passage after the primary culture was established. Three days after the culture was started the cells were poured with nutrient medium without serum added and after further 24 hours was replaced by nutrient medium with takalcitol and calcitriol in the defined concentrations. The expression of the genes coding histone H3 was evaluated with the use of RT-PCR technique.


RESULTS
Tacalcitiol and calcitriol in vitro decrease proliferation of fibroblasts sampled from nasal polyps. Inhibition is most effective for the concentration of 10-4M. Tacalcitiol and calcitriol also inhibit level of histone H3 gene expression.


CONCLUSIONS
Experimental data suggest tacalcitiol to be more effective in the same concentration. Present studies may indicate the direction of further investigation in the potential pharmacological treatment on nasal polyps.
Katedra i Klinika Otolaryngologii Akademii Medycznej we Wrocławiu. beatanm@wp.pl
Beata
B
Rostkowska-Nadolska
Dariusz
D
Kuśmierz
Małgorzata
M
Kapral
Małgorzata
M
Latocha
Leongina
L
Swiatkowska
Marcin
M
Fraczek
D002117:Calcitriol / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D004100:Dihydroxycholecalciferols / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D015870:Gene Expression; D006657:Histones / Q000378:metabolism*; D006801:Humans; D066298:In Vitro Techniques; D009298:Nasal Polyps / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014807:Vitamin D / Q000031:analogs & derivatives / Q000494:pharmacology; D014815:Vitamins / Q000494:pharmacology*
D004100:Dihydroxycholecalciferols; D006657:Histones; D014815:Vitamins; D014807:Vitamin D; C023850:1 alpha,24-dihydroxyvitamin D3; D002117:Calcitriol