Molecular systematics and biogeography of Nicrophorus in part--the investigator species group (Coleoptera: Silphidae) using mixture model MCMC.
Burying beetles (Silphidae: Nicrophorus) are well-known for their biparental care and monopolization of small vertebrate carcasses in subterranean crypts. They have been the focus of intense behavioral ecological research since the 1980s yet no thorough phylogenetic estimate for the group exists. The relationships among the species, and the validity of some species, are poorly understood. Here, we infer the relationships and examine species boundaries among 50 individuals representing 15 species, primarily of the investigator species group, using a mixture-model Bayesian analysis. Two mitochondrial genes, COI and COII, were used, providing 2129 aligned nucleotides (567 parsimony-informative). The Akaike Information Criterion and Bayes Factors were used to select the best fitting model, in addition to Reversible Jump MCMC, which accommodated model uncertainty. A 21 parameter, three-partition GTR+G was the final model chosen. Despite a presumed Old World origin for the genus itself, the basal lineages and immediate outgroups of the investigator species group are New World species. Bayesian methods reconstruct the common ancestor of the investigator species group as New World and imply one later transition to the Old World with two return transitions to the New World. Prior hypotheses concerning the questionable validity of four species names, Nicrophorus praedator, Nicrophorus confusus, Nicrophorus encaustus and Nicrophorus mexicanus were tested. No evidence was found for the validity of the Nicrophorus investigator synonym N. praedator. We found evidence rejecting the species status of N. confusus (NEW SYNONYM of Nicrophorus sepultor). Weak evidence was found for the species status of N. encaustus and N. mexicanus, which are tentatively retained as valid. Our results strongly reject a recently published hypothesis that Nicrophorus interruptus (NEW STATUS as valid species) is a subspecies of N. investigator.
Sikes
Derek S
DS
Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, Alta., Canada. dsikes@alaska.edu
Vamosi
Steven M
SM
Trumbo
Stephen T
ST
Ricketts
Marcia
M
Venables
Chandra
C
D000818:Animals; D001208:Asia; D001499:Bayes Theorem; D001517:Coleoptera / Q000145:classification / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D005060:Europe; D005843:Geography; D008969:Molecular Sequence Data; D009656:North America; D010802:Phylogeny; D017422:Sequence Analysis, DNA
D004272:DNA, Mitochondrial; D003576:Electron Transport Complex IV