Pressure-driven perfusion culture microchamber array for a parallel drug cytotoxicity assay.
This article reports a pressure-driven perfusion culture chip developed for parallel drug cytotoxicity assay. The device is composed of an 8 x 5 array of cell culture microchambers with independent perfusion microchannels. It is equipped with a simple interface for convenient access by a micropipette and connection to an external pressure source, which enables easy operation without special training. The unique microchamber structure was carefully designed with consideration of hydrodynamic parameters and was fabricated out of a polydimethylsiloxane by using multilayer photolithography and replica molding. The microchamber structure enables uniform cell loading and perfusion culture without cross-contamination between neighboring microchambers. A parallel cytotoxicity assay was successfully carried out in the 8 x 5 microchamber array to analyze the cytotoxic effects of seven anticancer drugs. The pressure-driven perfusion culture chip, with its simple interface and well-designed microfluidic network, will likely become an advantageous platform for future high-throughput drug screening by microchip.
Research Center of Advanced Bionics, National Institute of Advanced Industrial Science and Technology, Central 5th, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
Shinji
S
Sugiura
Jun-ichi
J
Edahiro
Kyoko
K
Kikuchi
Kimio
K
Sumaru
Toshiyuki
T
Kanamori
D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000009:adverse effects*; D001681:Biological Assay; D002452:Cell Count; D018929:Cell Culture Techniques / Q000295:instrumentation* / Q000379:methods; D002470:Cell Survival / Q000187:drug effects; D003470:Culture Media; D015194:Diffusion Chambers, Culture / Q000295:instrumentation / Q000379:methods; D004129:Dimethylpolysiloxanes; D004867:Equipment Design; D019544:Equipment Failure Analysis; D017022:Flow Injection Analysis; D006367:HeLa Cells; D006801:Humans; D006874:Hydrostatic Pressure; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics; D010477:Perfusion / Q000295:instrumentation
D000970:Antineoplastic Agents; D003470:Culture Media; D004129:Dimethylpolysiloxanes; C013830:baysilon