Quenching of the long-lived Ru(II)bathophenanthroline luminescence for the detection of supramolecular interactions.
A feasibility study based on tailor-made peptide sequences for a new robust luminescence probe-system using the long-lived luminescence of a Ru(II)-bathophenanthroline complex in combination with an efficient anthraquinone-type quencher is presented. Due do their high chemical stability, both dyes can be introduced during solid-phase peptide synthesis avoiding post-synthetic labelling. Photophysical measurements revealed an intense quenching of the luminescence of the Ru-complex (65-68%) which was also confirmed by calculations resulting from decay time measurements. The long-lived luminescence allows for a time-gated detection scheme, which can reduce any luminescence contribution from matrix components.
Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität Freiburg, Albertstr. 21, D-79104 Freiburg.
Rolf A
RA
Kramer
Eva K
EK
Kainmüller
Roman
R
Flehr
Michael U
MU
Kumke
Willi
W
Bannwarth
D000042:Absorption; D000595:Amino Acid Sequence; D000880:Anthraquinones / Q000737:chemistry; D004579:Electron Transport; D004735:Energy Transfer; D031541:Fluorescence Resonance Energy Transfer; D049449:Luminescence; D009942:Organometallic Compounds / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry; D010618:Phenanthrolines / Q000737:chemistry*; D013997:Time Factors
D000880:Anthraquinones; D009942:Organometallic Compounds; D010455:Peptides; D010618:Phenanthrolines