Growth of animal cells on microbeads. I. In situ estimation of numbers.
Evaluation of the number of anchorage-dependent cells growing on the surface of microbeads kept in suspension in a bioreactor is a tedious procedure fraught with many difficulties. Using the Coulter counter as a biomass probe, this article shows that the number of cells adhering to microbeads can be determined while the cells are still attached.
Laboratory of Molecular Biochemistry, Faculty of Medicine, Mons State University, Avenue du Champ de Mars, 24-7000, Mons, Belgium.
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Miller