Molecular characterization of polychlorinated biphenyl-dechlorinating populations in contaminated sediments.
Polychlorinated biphenyl (PCB)-dechlorinating microorganisms were characterized in PCB-contaminated sediments using amplified ribosomal DNA restriction analysis (ARDRA). The sediments were prepared by spiking Aroclor 1248 into PCB-free sediments, and were inoculated with microorganisms eluted from St. Lawrence River sediments. PCB-free sediments inoculated with the same inoculum served as the control. Four restriction fragment length polymorphism (RFLP) groups in the eubacterial and two in the archaeal domain were found exclusively in PCB-spiked sediment clone libraries. Sequence analysis of the four eubacterial clones showed homology to Escherichia coli, Lactosphaera pasteurii, Clostridium thermocellum, and Dehalobacter restrictus. The predominant archaeal sequence in the PCB-spiked sediment clone library was closely related to Methanosarcina barkeri, which appear to support earlier findings that methanogens are involved in PCB dechlorination. When the dot-blot hybridization was performed between the sediment DNA extract and the probes designed with eubacterial RFLP groups, the intensity of two of eubacterial RFLP groups, which showed high sequence homology to C. pascui and D. restrictus, was highly correlated with the number of dechlorinating microorganisms suggesting these two members intend to contribute to PCB dechlorination.
School of Biological Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.
Kyoung-Hee
KH
Oh
Ellen B
EB
Ostrofsky
Young-Cheol
YC
Cho
D001105:Archaea / Q000145:classification / Q000235:genetics; D001140:Aroclors / Q000378:metabolism; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002713:Chlorine / Q000378:metabolism; D019015:Geologic Sediments / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011078:Polychlorinated Biphenyls / Q000378:metabolism*; D012150:Polymorphism, Restriction Fragment Length; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000382:microbiology; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000378:metabolism*
D001140:Aroclors; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; C028617:aroclor 1248; D002713:Chlorine; D011078:Polychlorinated Biphenyls