Minimal chemical modification of reductive end of dextran to produce an amphiphilic polysaccharide able to incorporate onto lipid nanocapsules.
In order to graft an amphiphilic polysaccharide to lipid nanocapsules, we present here a new method of dextran lipidation. The lipidation strategy is based on the formation of an oxime linkage between the amphiphilic hydroxylamine C16E20ONH2 and the reductive end of a 40 kDa dextran. This chemoselective reaction allows us to control the lipidation site and the number of lipid introduced on the dextran molecule. This new amphiphilic dextran was used to coat the surface of lipid nanocapsules. The coating efficiency was followed by dynamic light scattering and the presence of the polysaccharide was confirmed by (1)H NMR and observed by electronic microscopy.
Laboratoire de Chimie et MicroNanotechnologie à Visée Thérapeutique, UMR 8161 CNRS-Université de Lille 2-Université de Lille 1-Institut Pasteur de Lille, France.
Antoine
A
Richard
Alexandre
A
Barras
Amena Ben
AB
Younes
Nicole
N
Monfilliette-Dupont
Patricia
P
Melnyk
D003911:Dextrans / Q000737:chemistry*; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry; D019811:Hydroxylamine / Q000737:chemistry; D008055:Lipids / Q000737:chemistry*; D053769:Nanocapsules / Q000737:chemistry*; D010084:Oxidation-Reduction
D003911:Dextrans; D004337:Drug Carriers; D008055:Lipids; D053769:Nanocapsules; D019811:Hydroxylamine