A functional variable number of tandem repeats is located at the 5' flanking region of the human secretin gene plays a downregulatory role in expression.
Secretin is a peptide hormone playing multiple functions in the brain-gut axis. In this report, we investigated, by promoter analysis, the potential function of the variable of tandem repeats (VNTR), located at the 5' upstream region of the human secretin gene, and we demonstrated for the first time that this VNTR could downregulate transcription of the human secretin gene in a promoter-specific manner. The efficiency of VNTR in silencing the promoter was found to be directly related the number of repetitive units residing within. We also showed the deoxyribonucleic acid sequence as well as the length polymorphism of the VNTR of 76 Chinese individuals. These results collectively suggest that VNTR could potentially be a functional regulator to control the expression of the human secretin gene in different individuals.
School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, China.
Leo T O
LT
Lee
Ian P Y
IP
Lam
Billy K C
BK
Chow
D024506:5' Flanking Region / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D015536:Down-Regulation; D005786:Gene Expression Regulation; D006801:Humans; D018598:Minisatellite Repeats; D008969:Molecular Sequence Data; D011401:Promoter Regions, Genetic; D012633:Secretin / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment
D012633:Secretin