Rhizobium rubi(T): a gram-negative phytopathogenic bacterium expressing the Lewis B epitope on the outer core of its lipooligosaccharide fraction.
The structure of the core oligosaccharide from the phytopathogenic bacterium Rhizobium rubi was deduced by combining information from complementary chemical approaches (alkaline and acid hydrolysis), similar to the "overlap peptide" strategy. This structure is new and it contains two main oligosaccharide backbones that differ in the substitution degree of the external Kdo unit. The relevant feature shared by both oligosaccharides is the presence of a tetrasaccharide motif that is similar to the blood group Lewis B antigen (Le(B)). This epitope differs from Le(B) in the glycosidic configuration of the glucosamine unit (alpha and not beta) and in the occurrence of acetyls substituents at O3 and/or O4 of the galactose moiety. Other notable structural features are the location of the Dha residue, the presence of a alpha-glucose unit that is linked to the inner Kdo unit, the high number of acid sugars and the highly branched core structure.
Department of Organic Chemistry and Biochemistry, University of Napoli "Federico II", Via Cintia 4, 80126 Napoli, Italy.
Valentina
V
Gargiulo
Domenico
D
Garozzo
Rosa
R
Lanzetta
Antonio
A
Molinaro
Luisa
L
Sturiale
Cristina
C
De Castro
Michelangelo
M
Parrilli
D000143:Acids / Q000737:chemistry; D000641:Ammonia / Q000737:chemistry; D002462:Cell Membrane / Q000737:chemistry; D000939:Epitopes / Q000737:chemistry* / Q000378:metabolism*; D006868:Hydrolysis; D007983:Lewis Blood Group Antigens / Q000737:chemistry*; D008070:Lipopolysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry; D010935:Plant Diseases / Q000382:microbiology*; D010944:Plants; D012231:Rhizobium / Q000166:cytology / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000143:Acids; D000939:Epitopes; D007983:Lewis Blood Group Antigens; D008070:Lipopolysaccharides; D009844:Oligosaccharides; C023023:lipid-linked oligosaccharides; D000641:Ammonia