Resection-induced intestinal adaptation and the role of enteric smooth muscle.
BACKGROUND
Intestinal adaptation after massive small bowel resection (SBR) involves all layers of the bowel wall. Most prior work has focused on changes that occur in the intestinal mucosa. However, the contribution of the underlying intestinal smooth muscle (ISM) to the overall adaptation response remains unclear.


METHODS
Male C57BL/6 or waved-2 (diminished activity of the epidermal growth factor receptor) mice underwent a 50% proximal SBR or sham operation, and the remnant ileum was harvested 3, 7, and 28 days. Markers of adaptation (villus height, bowel length, circumference, and ISM thickness) and ISM proliferation were recorded. Contractility was measured by attaching the distal ileum to strain gauge transducers and exposed to varying doses of carbachol.


RESULTS
Intestinal smooth muscle thickness was unchanged at any given time-point after resection; however, the bowel caliber and length were increased, and augmented rates of ISM proliferation were identified. Contractility was increased at 7 days after SBR. Waved-2 mice demonstrated minimal proliferation or intestinal lengthening in response to SBR.


CONCLUSIONS
Compared with resection-induced thickening of the mucosa, proliferative changes in the ISM are unique and primarily affect bowel caliber, length, and contractility. Epidermal growth factor receptor signaling appears to play a significant role in adaptation of the ISM cellular compartment.
Martin
Colin A
CA
Department of Surgery, Division of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, OH 45729, USA.
Bernabe
Kathryn Q
KQ
Taylor
Janice A
JA
Nair
Rajalakshmi
R
Paul
Richard J
RJ
Guo
Jun
J
Erwin
Christopher R
CR
Warner
Brad W
BW
D000222:Adaptation, Physiological; D000704:Analysis of Variance; D000818:Animals; D004195:Disease Models, Animal; D007082:Ileum / Q000601:surgery*; D007150:Immunohistochemistry; D007413:Intestinal Mucosa / Q000473:pathology / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009119:Muscle Contraction / Q000502:physiology; D009126:Muscle Relaxation / Q000502:physiology; D009130:Muscle, Smooth / Q000473:pathology / Q000502:physiology*; D010528:Peristalsis / Q000502:physiology*; D011336:Probability; D011897:Random Allocation; D018570:Risk Assessment; D012680:Sensitivity and Specificity; D012778:Short Bowel Syndrome / Q000503:physiopathology*