Miniaturized probe based on a microelectromechanical system mirror for multiphoton microscopy.
A factor that limits the use of multiphoton microscopy (MPM) in clinical and preclinical studies is the lack of a compact and flexible probe. We report on a miniaturized MPM probe employing a microelectromechanical system (MEMS) scanning mirror and a double-clad photonic crystal fiber (DCPCF). The use of a MEMS mirror and a DCPCF provides many advantages, such as size reduction, rapid and precise scanning, efficient delivery of short pulses, and high collection efficiency of fluorescent signals. The completed probe was 1 cm in outer diameter and 14 cm in length. The developed probe was integrated into an MPM system and used to image fluorescent beads, paper, and biological specimens.
Jung
Woonggyu
W
Beckman Laser Institute, University of California, Irvine, CA 92697, USA.
Tang
Suo
S
McCormic
Daniel T
DT
Xie
Tiquiang
T
Ahn
Yeh-Chan
YC
Su
Jianping
J
Tomov
Ivan V
IV
Krasieva
Tatiana B
TB
Tromberg
Bruce J
BJ
Chen
Zhongping
Z
D000818:Animals; D002467:Cell Nucleus / Q000648:ultrastructure; D019902:Chondrocytes / Q000648:ultrastructure*; D006801:Humans; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation / Q000379:methods