Low positive predictive value of a nucleic acid amplification test for nongenital Neisseria gonorrhoeae infection in homosexual men.
The diagnosis of nongenital Neisseria gonorrhoeae infection by the Becton Dickinson ProbeTec ET Chlamydia trachomatis and N. gonorrhoeae Amplified DNA assay had low positive predictive value among an Australian community-based sample of homosexual men. Only 30.4% of oropharyngeal samples and 73.7% of anorectal samples were positive for N. gonorrhoeae by the porA assay. The accuracy of nucleic acid amplification tests in this context is compromised without supplemental testing.
McNally
Leon P
LP
Molecular Diagnostic Medicine Laboratory, SydPath, St. Vincent's Hospital, Darlinghurst, NSW. lmcnally@stvincents.com.au
Templeton
David J
DJ
Jin
Fengyi
F
Grulich
Andrew E
AE
Donovan
Basil
B
Whiley
David M
DM
Cunningham
Philip H
PH
D001003:Anal Canal / Q000382:microbiology*; D015331:Cohort Studies; D004269:DNA, Bacterial / Q000032:analysis; D005189:False Positive Reactions; D006069:Gonorrhea / Q000175:diagnosis*; D018451:Homosexuality, Male; D006801:Humans; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000235:genetics / Q000302:isolation & purification*; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation / Q000379:methods*; D009960:Oropharynx / Q000382:microbiology*; D018272:Porins / Q000235:genetics; D011237:Predictive Value of Tests
D004269:DNA, Bacterial; D018272:Porins; C057699:porin protein, Neisseria