Clinical determination of glucose in human serum by a tomato skin biosensor.
BACKGROUND
Glucose biosensors based on enzyme reaction of glucose oxidase were studied because the symptomatic therapy of diabetes mellitus requires reliable assessment of blood glucose level at frequent intervals. Tomato skin membranes have been successfully employed to entrap glucose oxidase for fabrication of glucose biosensor.


METHODS
Glucose oxidase was immobilized onto the tomato skin and the enzyme membrane was then positioned on the surface of an oxygen electrode. The glucose concentration was quantified by the change of dissolved oxygen. All the serum samples were also simultaneously determined by a Hitachi 7060 chemistry analyzer.


RESULTS
The response of the biosensor showed a linear relationship with a concentration range of 1.0-30.0 mmol/l glucose. The limit of detection was 0.20 mmol/l. Error Grid analysis demonstrated that 100% of the results fell within clinically acceptable zones A and B. The F- and t-tests showed no significant differences between the 2 methods. The recovery was 95.0-110.0% for 30 serum samples analysis.


CONCLUSIONS
The tomato skin biosensor possesses the advantages of simple fabrication, fast response time, low cost and high sensitivity. The results of our method are more accurate than and match well with the current clinical instrument method.
College of Chemistry, Sichuan University, Chengdu, People's Republic of China.
Hui
H
Han
Yi
Y
Li
Huan
H
Yue
Zaide
Z
Zhou
Dan
D
Xiao
Martin M F
MM
Choi
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D001786:Blood Glucose / Q000032:analysis*; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005949:Glucose Oxidase / Q000737:chemistry*; D006801:Humans; D018551:Solanum lycopersicum; D010100:Oxygen / Q000737:chemistry; D019441:Plant Epidermis / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013997:Time Factors
D001786:Blood Glucose; D004800:Enzymes, Immobilized; D005949:Glucose Oxidase; D010100:Oxygen