Sulfated galactan is a catalyst of antithrombin-mediated inactivation of alpha-thrombin.
Novel compounds presenting anticoagulant activity, such as sulfated polysaccharides, open new perspectives in medicine. Elucidation of the molecular mechanism behind this activity is desirable by itself, as well as because it allows for the design of novel compounds. In the present study, we investigated the action of an algal sulfated galactan, which potentiates alpha-thrombin inactivation by antithrombin. Our results indicate the following: 1) both the sulfated galactan and heparin potentiate protease inactivation by antithrombin at similar molar concentrations, however they differ markedly in the molecular size required for their activities; 2) this galactan interacts predominantly with exosite II on alpha-thrombin and, similar to heparin, catalyzes the formation of a covalent complex between antithrombin and the protease; 3) the sulfated galactan has a higher affinity for alpha-thrombin than for antithrombin. We propose that the preferred pathway of sulfated galactan-induced inactivation of alpha-thrombin by antithrombin starts with the polysaccharide binding to the protease through a high-affinity interaction. Antithrombin is then added to the complex and the protease is inactivated by covalent interactions. Finally, the antithrombin-alpha-thrombin covalent complex dissociates from the polysaccharide chain. This mechanism resembles the action of heparin with low affinity for antithrombin, as opposed to heparin with high affinity for serpin.
Melo
Fábio R
FR
Laboratório de Tecido Conjuntivo, Hospital Universitário Clementino Fraga Filho, Universidade Federal do Rio de Janeiro, Caixa Postal 68041, Rio de Janeiro, RJ, 21941-590, Brazil.
Pereira
Mariana S
MS
Monteiro
Robson Q
RQ
Foguel
Débora
D
Mourão
Paulo A S
PA
D000991:Antithrombins / Q000494:pharmacology*; D001665:Binding Sites; D002384:Catalysis / Q000187:drug effects; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D004591:Electrophoresis, Polyacrylamide Gel; D004789:Enzyme Activation / Q000187:drug effects; D005685:Galactans / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D006629:Hirudins / Q000494:pharmacology; D006801:Humans; D010446:Peptide Fragments / Q000494:pharmacology; D013463:Sulfuric Acid Esters / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D013917:Thrombin / Q000378:metabolism*
D000991:Antithrombins; D005685:Galactans; D006629:Hirudins; D010446:Peptide Fragments; D013463:Sulfuric Acid Esters; D013917:Thrombin