Effects of nicotinamide and riboflavin on the biodesulfurization activity of dibenzothiophene by Rhodococcus erythropolis USTB-03.
Rhodococcus erythropolis USTB-03 is a promising bacterial strain for the biodesulfurization of dibenzothiophene (DBT) via a sulfur-specific pathway in which DBT is converted to 2-hydroxybiphenyl (2HBP) as an end product. The effects of nicotinamide and riboflavin on the sulfur specific activity (SA) of DBT biodesulfurization by R. erythropolis USTB-03 were investigated. Both nicotinamide and riboflavin were found to enhance the expression of SA, which was not previously reported. When R. erythropolis USTB-03 was grown on a medium containing nicotinamide of 10.0 mmol or riboflavin of 50.0 micromol, SA was raised from 68.0 or so to more than 130 mmol 2HBP/(kg dry cells. x ). When R. erythropolis USTB-03 was grown in the presence of both nicotinamide of 5.0 mmol and riboflavin of 25.0 Cmicrool, SA was further increased to 159.0 mmol 2HBP/(kg dry cells. x ). It is suggested that the biological synthesis of reduced form of flavin mononucleotide (FMNH2), an essential coenzyme for the activities of biodesulfurization enzyme Dsz C and A, might be enhanced by nicotinamide and riboflavin, which was responsible for the increased SA of R. erythropolis USTB-03.
Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China. haiyan@sas.ustb.edu.cn
Hai
H
Yan
Xudong
X
Sun
Qianqian
Q
Xu
Zhao
Z
Ma
Chengbin
C
Xiao
Ning
N
Jun
D001673:Biodegradation, Environmental / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D009536:Niacinamide / Q000494:pharmacology*; D012240:Rhodococcus / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D012256:Riboflavin / Q000494:pharmacology*; D013455:Sulfur / Q000378:metabolism; D013876:Thiophenes / Q000378:metabolism*
D013876:Thiophenes; D009536:Niacinamide; D013455:Sulfur; D012256:Riboflavin; C016366:dibenzothiophene