Deactivation of free and stabilized acid phosphatase by urea.
Tests on acid phosphatase (E.G. 3.1.3.2) deactivation by urea have been performed at two pH values. Two conditions have been used: native enzyme operating batch-wise in dilute solution and stabilized enzyme in continuous flow ultrafiltration membrane reactor. Stabilization is achieved by confining the enzyme within a concentrated solution of a linear chain polymer that forms a polarization layer over the membrane. The results provide significant information on the kinetics and thermodynamics of the complex phenomena taking place during deactivation. Deactivation by urea is also compared with thermal deactivation.
Dipartimento di Ingegneria Chimica, Universitá di Napoli, Piazzale V. Tecchio, 1-80125 Napoli, Italy.
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Gianfreda
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Marrucci
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Greco