Leukotriene C4 release and gene expressions of IL-8 and MCP-1 in porcine alveolar epithelial type II cells.
Leukotrienes (LT) and chemokines are important chemotactic compounds in regulating the recruitment and activation of immune cells during pulmonary inflammatory reactions. Results showed that LTC4 release by porcine alveolar epithelial type II cells (AEC IIs) is significantly enhanced by either LTB4 or LPS stimulation. The basal level of IL-8 gene expression in AEC IIs was only 1/3 of that observed in alveolar macrophages (AMs) while AEC IIs expressed a higher basal level of monocyte chemotactic peptide-1 (MCP-1) and also in response to LPS stimulation than do AMs. The increasing basal and LT-induced MCP-1 gene expressions after 8h of incubation were observed in AEC IIs but decreased in AMs. These findings suggest that AEC IIs play an important role in initial inflammatory reactions of the lung by releasing LTC4, and that they also modulate later inflammatory reactions, evidenced by consistent elevation of MCP-1 gene expression after and during exogenous challenge in pigs.
Department of Veterinary Medicine, National Pingtung University of Science and Technology, 1 Hsueh Fu Road, Nei Pu Hsiang, 91207 Pingtung, Taiwan, ROC. hcchaung@mail.npust.edu.tw
Hso-Chi
HC
Chaung
Yi
Y
Lien
Wen-Bin
WB
Chung
D000818:Animals; D002450:Cell Communication / Q000276:immunology; D018932:Chemokine CCL2 / Q000096:biosynthesis* / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D015870:Gene Expression; D016209:Interleukin-8 / Q000096:biosynthesis* / Q000235:genetics; D017997:Leukotriene C4 / Q000096:biosynthesis*; D016676:Macrophages, Alveolar / Q000276:immunology / Q000473:pathology; D008297:Male; D011014:Pneumonia / Q000276:immunology / Q000662:veterinary*; D011650:Pulmonary Alveoli / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine; D013553:Swine Diseases / Q000276:immunology*
D018932:Chemokine CCL2; D016209:Interleukin-8; D012333:RNA, Messenger; D017997:Leukotriene C4