Effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent mouse embryo development.
OBJECTIVE
To investigate the effect of a phosphodiesterase type 3 inhibitor in oocyte maturation medium on subsequent embryo development.


METHODS
Animal study.


METHODS
University laboratory.


METHODS
CD-1 mice.


METHODS
Immature oocytes were arrested meiotically in medium containing a specific phosphodiesterase type 3 inhibitor, cilostamide (1 micromol/L, 5 micromol/L, and 10 micromol/L), for 6 and 24 hours of prematuration culture, and then matured in vitro in inhibitor-free medium.


METHODS
Oocyte maturation, fertilization, and early embryonic development in vitro.


RESULTS
After 6 and 24 hours of prematuration culture with 1 micromol/L, 5 micromol/L, and 10 micromol/L cilostamide, there were no differences in maturation, fertilization, and early embryonic development compared with non-arrested controls.


CONCLUSIONS
Prematuration culture with cilostamide, for either 6 or 24 hours, may not be beneficial for subsequent embryonic development of immature mouse oocytes.
Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, South Korea.
Byung Chul
BC
Jee
Hai-Ying
HY
Chen
Ri-Cheng
RC
Chian
D000818:Animals; D001755:Blastocyst / Q000187:drug effects / Q000502:physiology; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology*; D005260:Female; D005306:Fertilization / Q000187:drug effects / Q000502:physiology; D006064:Gonadotropins, Equine / Q000494:pharmacology; D008540:Meiosis / Q000187:drug effects; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology*; D011247:Pregnancy; D015363:Quinolones / Q000494:pharmacology*
D006064:Gonadotropins, Equine; D010726:Phosphodiesterase Inhibitors; D015363:Quinolones; C021294:cilostamide