The effect of Cecropia glazioui SNETHLAGE on the physical and neurobehavioral development of rats.
This study aimed to determine the LD50, toxic effects on organogenesis/fetal-development, physical aspects, and developmental/neurobehavioral reflexes of litters previously exposed to Cecropia glazioui SNETHL (Cg) extract during the entire gestational period. Swiss mice were submitted to doses of 0.5, 1.0, 2.5 and 5.0 g/Kg/p.o. Female rats received 1.0 g/Kg/day of Cg extract (G1, n = 10) or 1.0 mL/Kg/day of deionized water (G2, n = 10) during pregnancy. The number of successful gestations, pregnant females weight and born/dead-born offsprings were evaluated. Physical development (offspring weight; fluff and hair appearing; ear unsticking and opening; incisor teeth eruption; eyes opening; testis descent; vagina opening; rearing frequency; uprightness latency and negative geotaxis) and the sleeping time (30 mg/Kg/i.p. sodium pentobarbital assay) were also observed. Open field assay evaluated the developmental/neurobehavioral reflexes of pups. LD50 was higher than 5.0 g/Kg. The extract did not affect the gestation number, born/dead-born offspring number and the female weight during pregnancy. The weight and the physical development of both genders pups were not affected (p > 0.05), but the uprightness latency and the negative geotaxis reflexes were enhanced and the rearing frequency decreased (p < 0.05). Ambulation, cleaning activity, sleeping time, and immobility were not affected (p > 0.05). We concluded that Cg extract showed low toxicity to pregnant rats and their litters.
University of Sorocaba, Pharmacology Department, Sorocaba, Brazil. marli.gerenutti@uniso.br
M
M
Gerenutti
A F Rollo Oliveira
AF
Prestes
M Glauzer
MG
Silva
F De Sa
Fde S
Del Fiol
Y Oshima
YO
Franco
P C
PC
Venancio
F C
FC
Groppo
D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D001724:Birth Weight / Q000187:drug effects; D005260:Female; D047109:Fetal Development / Q000187:drug effects; D006128:Growth / Q000187:drug effects*; D006993:Hypnotics and Sedatives / Q000494:pharmacology; D007928:Lethal Dose 50; D009043:Motor Activity / Q000187:drug effects; D010424:Pentobarbital / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D004856:Postural Balance / Q000187:drug effects; D011247:Pregnancy; D051381:Rats; D012098:Reproduction / Q000187:drug effects; D012890:Sleep / Q000187:drug effects; D027003:Urticaceae / Q000737:chemistry* / Q000633:toxicity; D015430:Weight Gain / Q000187:drug effects
D006993:Hypnotics and Sedatives; D010936:Plant Extracts; D010424:Pentobarbital