CrAsH-quantum dot nanohybrids for smart targeting of proteins.
Smart nanohybrids were prepared by conjugation of CrAsH to hydrosoluble and biocompatible quantum dots (QDs). The resulting probes were shown to bind efficiently and selectively to Cys-tagged proteins. The interaction with the protein was detected by an increase of the fluorescence emission of CrAsH. While the latter faded rapidly under continuous excitation, emission of the QD remained unaffected. The persistent fluorescence of the QD should thus allow extended monitoring of the target protein.
Genin
Emilie
E
CEA, iBiTecS, Service de Chimie Bioorganique et de Marquage, 91191 Gif sur Yvette, France.
Carion
Olivier
O
Mahler
Benoit
B
Dubertret
Benoit
B
Arhel
Nathalie
N
Charneau
Pierre
P
Doris
Eric
E
Mioskowski
Charles
C
D001672:Biocompatible Materials / Q000737:chemistry*; D003545:Cysteine / Q000737:chemistry; D005452:Fluoresceins / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D010743:Phospholipids / Q000737:chemistry; D045663:Quantum Dots; D011994:Recombinant Proteins / Q000737:chemistry*
D001672:Biocompatible Materials; C518706:CrAsH compound; D005452:Fluoresceins; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D010743:Phospholipids; D011994:Recombinant Proteins; C024098:6-carboxyfluorescein; D003545:Cysteine