The immobilization of animal cells using the cysteine-modified RGD oligopeptide.
RGD peptide sequence is an effective cell recognition motif and used to enhance the cell adhesion on desired solid material for cell immobilization. We have synthesized CRGD, CRGD-multiple-armed peptide (MAP), RGD-MAP-C and evaluated their comparative efficacy for cell immobilization. Each peptide was assembled on gold surface and investigated by the atomic force microscopy (AFM) technique in the contact mode. The viability of immobilized animal cells was examined by an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Our results showed that RGD-MAP-C in comparison to others was the most effective proliferation of cells on the gold surface. The goal of this present work is integration to the nano-pattern cell chip bioplatform for biomedical assays or provide valuable insights into cell biology and design of biomaterials. This RGD-MAP-C can be applicable to the nano-pattern cell chip platform.
Department of Chemical & Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
Cheol-Heon
CH
Yea
Bumhwan
B
Lee
Hyunhee
H
Kim
Sang-Uk
SU
Kim
Waleed Ahmed
WA
El-Said
Junhong
J
Min
Byung-Keun
BK
Oh
Jeong-Woo
JW
Choi
D000595:Amino Acid Sequence; D000818:Animals; D002455:Cell Division; D002470:Cell Survival; D002477:Cells / Q000378:metabolism* / Q000648:ultrastructure; D018914:Cells, Immobilized / Q000648:ultrastructure; D003545:Cysteine / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D007091:Image Processing, Computer-Assisted; D008853:Microscopy / Q000295:instrumentation / Q000379:methods; D018625:Microscopy, Atomic Force; D009842:Oligopeptides / Q000737:chemistry* / Q000235:genetics; D013778:Tetrazolium Salts / Q000378:metabolism; D013844:Thiazoles / Q000378:metabolism
D009842:Oligopeptides; D013778:Tetrazolium Salts; D013844:Thiazoles; C047981:arginyl-glycyl-aspartic acid; C022616:thiazolyl blue; D003545:Cysteine