A rapid and sensitive chemiluminescent immunoassay of total thyroxin with DMAE x NHS-Labeled.
A novel chemiluminescence immunoassay (CLIA) of total thyroxin (TT4) with the acridinium ester of DMAE x NHS [2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-10-methyl-acridinium-9-carboxylate methosulfate] labeled has been developed. In our method, microwells were coated with anti-T4 monoclonal antibody (McAb) and DMAE x NHS synthesized by our laboratory was conjugated with streptavidin (SA). T4-BSA was conjugated with biotin-N-hydroxysuccinimide (B x NHS), T4-BSA- B x NHS and the T4 in the standard or sample competitively react with anti-T4 McAb. Streptavidin-biotin separation and enhanced techniques were applied in this assay. The presented approach shows many excellent characteristics, particularly, rapid assay process and high detection sensitivity.
Shanghai Applied Radiation Institute, College of Environmental and Chemical Engineering, Shanghai University, Jiadin, PR China.
Dongguang
D
Yin
Daxiang
D
Cui
Feng
F
Gao
Rong
R
He
Youfeng
Y
He
Yibing
Y
Liu
Decun
D
Shen
Minghong
M
Wu
D000166:Acridines; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D008163:Luminescent Measurements / Q000379:methods*; D012680:Sensitivity and Specificity; D013463:Sulfuric Acid Esters; D013959:Thyroid Diseases / Q000175:diagnosis; D013974:Thyroxine / Q000097:blood*
C530833:2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-10-methyl-acridinium-9-carboxylate methosulfate; D000166:Acridines; D013463:Sulfuric Acid Esters; D013974:Thyroxine