Simultaneous compartmentalization of lead and arsenic in co-hyperaccumulator Viola principis H. de Boiss.: an application of SRXRF microprobe.
The cellular distributions of Pb and As in the leaves of co-hyperaccumulator Viola principis H. de Boiss. were inspected by synchrotron X-ray fluorescence spectroscopy (SRXRF). The results revealed that Pb and As had similar compartmentalization patterns in the leaves. Both elements were enriched in the bundle sheath and the palisade mesophyll. In comparison with the sheath and the mesophyll, the vascular bundle and the epidermis contained lower levels of Pb and As. The palisade enrichment of Pb and As indicated that V. principis H. de Boiss. may have a special mechanism on detoxification of toxic metals within the mesophyll cells. Relative concentrations of both Pb and As in trichome bases were higher than those in trichome rays. The results of hierarchical cluster analysis and correlation analysis confirmed that the distribution of Pb was similar to that of As in the leaves, and their distribution patterns were different from the nutrient elements, such as K, Ca, Mn, Fe, Ni, Cu and Zn. In vivo cellular localization of Pb and As in the leaves provides insight into the physiological mechanisms of metal tolerance and hyperaccumulation in the hyperaccumulators.
Lei
Mei
M
Center for Environmental Remediation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, 11A Datun Road, Beijing 100101, PR China.
Chen
Tong-Bin
TB
Center for Environmental Remediation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, 11A Datun Road, Beijing 100101, PR China. Electronic address: chentb@igsnrr.ac.cn.
Huang
Ze-Chun
ZC
Center for Environmental Remediation, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, 11A Datun Road, Beijing 100101, PR China.
Wang
Yao-Dong
YD
Applied Ecology Research Group, School of Botany, The University of Melbourne, Victoria 3010, Australia.
Huang
Yu-Ying
YY
Laboratory of Synchrotron Radiation, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100039, PR China.
D001151:Arsenic / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002118:Calcium / Q000378:metabolism; D003300:Copper / Q000378:metabolism; D004784:Environmental Monitoring; D007501:Iron / Q000378:metabolism; D007854:Lead / Q000378:metabolism*; D008345:Manganese / Q000378:metabolism; D009532:Nickel / Q000378:metabolism; D018515:Plant Leaves / Q000166:cytology / Q000378:metabolism; D011188:Potassium / Q000378:metabolism; D012989:Soil Pollutants / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission / Q000295:instrumentation* / Q000379:methods; D017356:Synchrotrons; D032413:Viola / Q000166:cytology / Q000378:metabolism*
D012989:Soil Pollutants; D007854:Lead; D008345:Manganese; D003300:Copper; D009532:Nickel; D007501:Iron; D001151:Arsenic; D011188:Potassium; D002118:Calcium