Analysis of Salmonella Agona and Salmonella Weltevreden in Malaysia by PCR fingerprinting and antibiotic resistance profiling.
Forty-eight strains of Salmonella enterica subsp. enterica serovar Agona and 33 strains of Salmonella enterica subsp. enterica serovar Weltevreden were characterized by random amplified polymorphic DNA (RAPD) fingerprinting using 3 different arbitrary primer, Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR) and antimicrobial susceptibility testing. By using RAPD, 81 strains (44 strains of S. Agona and 33 strains of S. Weltevreden) can be clustered into 14 groups and 6 single isolates whereas ERIC-PCR produced 7 clusters and 3 single isolates. Thirteen antimicrobial agents were used and all the isolates were resistant to erythromycin and showed Multiple Antimicrobial Resistance indexes, ranging from 0.08 to 0.62. Poultry still remain as the common reservoir for multi-drug-resistant Salmonella. On the other hand, vegetables contaminated with S. Weltevreden showed a gain in antimicrobial resistance. Besides that, consistent antibiograms were observed from S. Weltevreden isolated at Kajang wet market on 2000/08/02.
Learn-Han
Lee
L
Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia, UPM Serdang, Selangor Darul Ehsan, Malaysia.
Yoke-Kqueen
Cheah
C
Salleh
Noorzaleha Awang
NA
Sukardi
Sabrina
S
Jiun-Horng
Sim
S
Chai-Hoon
Khoo
K
Radu
Son
S
D000818:Animals; D001483:Base Sequence; D016172:DNA Fingerprinting; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024881:Drug Resistance, Bacterial / Q000235:genetics; D005516:Food Microbiology; D006801:Humans; D008296:Malaysia; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D011200:Poultry / Q000382:microbiology; D019105:Random Amplified Polymorphic DNA Technique; D012478:Salmonella Food Poisoning / Q000382:microbiology; D019779:Salmonella enterica / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000472:pathogenicity; D012703:Serotyping; D013045:Species Specificity
D017931:DNA Primers; D004269:DNA, Bacterial