Microarray for molecular typing of Salmonella enterica serovars.
We describe the development of a spotted array for the delineation of the most common 14 disease-causing Salmonella serovars in the United States. Our array consists of 414 70 mers targeting core genes of Salmonella enterica, subspecies I specific genes, fimbrial genes, pathogenicity islands, Gifsy elements and other variable genes. Using this array we were able to identify a unique gene presence/absence profile for each of the targeted serovar which was used as the serovar differentiating criteria. Based on this profile, we developed a Matlab programme that compares the profile of an unknown sample to all 14 reference serovar profiles and give out the closest serovar match. Since we have included probes targeting most of the virulence genes and variable genes in Salmonella, in addition to using for serovar detection this array could also be used for studying the virulence gene content and also for evaluating the genetic relation between different isolates of Salmonella.
Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Joy
J
Scaria
Raghavan U M
RU
Palaniappan
David
D
Chiu
Julie Ann
JA
Phan
Lalit
L
Ponnala
Patrick
P
McDonough
Yrjo T
YT
Grohn
Steffen
S
Porwollik
Michael
M
McClelland
Chien-Shun
CS
Chiou
Chishih
C
Chu
Yung-Fu
YF
Chang
D016000:Cluster Analysis; D015342:DNA Probes / Q000145:classification / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D019779:Salmonella enterica / Q000235:genetics*; D013379:Substrate Specificity
D015342:DNA Probes