[Effects of Xiehuo Bushen Decoction on survival and differentiation of transplanted neural stem cells in brains of rats with intracerebral hemorrhage].
OBJECTIVE
To investigate the effects of Xiehuo Bushen Decoction (XHBSD), a compound Chinese herbal medicine, on the survival and differentiation of transplanted neural stem cells (NSCs) in brains of rats with intracerebral hemorrhage, and to explore the mechanism of Xiehuo Bushen formula in promoting the survival of transplanted NSCs.


METHODS
NSCs separated from hippocampuses of neonatal SD rats were cultured. Sixty-five panel reactive antibody (PRA) positive SD rats were selected by lymphocytotoxicity methods. The PRA positive rats were made into intracerebral hemorrhagic model and divided into three groups: cerebral hemorrhage group (n=15), NSCs transplanted group (n=25) and XHBSD group (n=25). XHBSD was orally administered after 5-bromodeoxyuridine (BrdU)-marked NSCs were transplanted in brains of rats with intracerebral hemorrhage in the XHBSD group. Rats in the other two groups were administered distilled water. The expressions of interferon gamma (IFN-gamma) and interleukin-4 (IL-4) mRNAs were measured by reverse transcription polymerase chain reaction (RT-PCR); the numbers of BrdU and 200 kD neurofilament (NF200) positive cells were detected by double-labeling immunofluorescence method.


RESULTS
The expression of IFN-gamma mRNA was down-regulated significantly in the XHBSD group, but the expression of IL-4 mRNA was up-regulated significantly (P<0.05). The numbers of BrdU and NF200 positive cells were also increased remarkably in the XHBSD group.


CONCLUSIONS
XHBSD can promote the survival and differentiation of transplanted NSCs, which may be related to inducing the expression of IL-4 mRNA and inhibiting the expression of IFN-gamma mRNA.
Zhong
Peng-cheng
PC
Department of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, China.
Li
Xing-qun
XQ
Liang
Qing-hua
QH
Luo
Wen-fang
WF
Liu
Qing-er
QE
Luo
Jie-kun
JK
Tang
Tao
T
Lin
Yuan
Y
D000818:Animals; D000831:Animals, Newborn; D001921:Brain / Q000601:surgery; D002454:Cell Differentiation / Q000187:drug effects*; D002543:Cerebral Hemorrhage / Q000188:drug therapy* / Q000601:surgery*; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D009474:Neurons / Q000166:cytology; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D033581:Stem Cell Transplantation
D004365:Drugs, Chinese Herbal