A RECQ5-RNA polymerase II association identified by targeted proteomic analysis of human chromatin.
Although the active forms of factors involved in DNA-related processes such as DNA replication, repair, and transcription are associated with chromatin, proteins are rarely purified from this source. Here, we describe a protocol for the isolation of chromatin-associated factors and use it to identify proteins interacting with human RNA polymerase II (RNAPII). Our data establish RECQ5 helicase as a bona fide RNAPII-associated protein. The RECQ5-RNAPII interaction is direct and is mediated by the RPB1 subunit of RNAPII, and RECQ5 appears to be the only member of the human RECQ family of helicases that associates with RNAPII. These data suggest an unexpected role for RECQ5 helicase at the interface of transcription and genomic stability.
Aygün
Ozan
O
Mechanisms of Transcription Laboratory, Clare Hall Laboratories, Cancer Research UK London Research Institute, South Mimms, Hertfordshire EN6 3LD, United Kingdom.
Svejstrup
Jesper
J
Liu
Yilun
Y
D002478:Cells, Cultured; D002843:Chromatin / Q000201:enzymology*; D042822:Genomic Instability; D006801:Humans; D040901:Proteomics; D012319:RNA Polymerase II / Q000378:metabolism*; D053484:RecQ Helicases / Q000378:metabolism*; D014158:Transcription, Genetic
D002843:Chromatin; D012319:RNA Polymerase II; D053484:RecQ Helicases