Bioactive constituent production in St. John's Wort in vitro hairy roots. Regenerated plant lines.
A wild strain of Agrobacterium rhizogenes was used to regenerate twelve in vitro plant lines from different hairy roots of H. perforatum (St. John's Wort). The production of the main bioactive constituents was observed even though their yields varied in the different plant lines. Two lines were selected for the hyperoside production (4.9-4.6 mg/gdw) while nine were characterized by significant yields of chlorogenic acid (ranged from 0.47 to 1.09 mg/gdw). Furthermore, one out of twelve lines showed a 10-fold higher hypericin content (0.25 mg/gdw) than that reported for the in vitro shoots in the literature. Morphological and phytochemical features were determined in order to select H. perforatum genotypes enriched in valuable bioactive compounds.
Bertoli
Alessandra
A
Dipartimento di Chimica Bioorganica e Biofarmacia (DCBB), Università di Pisa, via Bonanno 33, 56126 Pisa, Italy. bertoli@farm.unipi.it
Giovannini
Annalisa
A
Ruffoni
Barbara
B
Guardo
Angela Di
AD
Spinelli
Guido
G
Mazzetti
Michele
M
Pistelli
Luisa
L
D000873:Anthracenes; D001643:Bridged Bicyclo Compounds / Q000032:analysis / Q000378:metabolism; D002726:Chlorogenic Acid / Q000032:analysis / Q000378:metabolism; D020902:Hypericum / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D010569:Perylene / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D010696:Phloroglucinol / Q000031:analogs & derivatives / Q000032:analysis / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D013729:Terpenes / Q000032:analysis / Q000378:metabolism; D014170:Transformation, Genetic
D000873:Anthracenes; D001643:Bridged Bicyclo Compounds; D010936:Plant Extracts; D013729:Terpenes; D002726:Chlorogenic Acid; D010569:Perylene; C004965:hypericin; D010696:Phloroglucinol; C001654:hyperforin