Determination of total dietary fiber in selected foods containing resistant maltodextrin by a simplified enzymatic-gravimetric method and liquid chromatography: interlaboratory study in China.
An interlaboratory study was conducted in China to validate the modified AOAC Official Method 2001.03 for the determination of total dietary fiber (TDF) in foods containing resistant maltodextrin (RMD), which will be adopted as the National Standard Method of China. The kind of buffer solution, the volume of filtrate evaporation, the volume of eluent for desalting and residual solution after evaporation, etc. were modified, which had been proved to have acceptable accuracy and precision in the routine assay. TDF contents in 3 representative foods and 2 kinds of RMD ingredient (i.e., NUTRIOSE 06 and NUTRIOSE 10) were measured using the modified method in 6 eligible laboratories representing commercial, industrial, and governmental laboratories in China. The results of the interlaboratory study indicated that the intralaboratory repeatability, interlaboratory reproducibility, and precision of the modified method are adequate for reliable analysis of TDF in food containing RMD, as well as resistant dextrin. Compared to AOAC Official Method 2001.03, the modified method is time- and cost-saving.
National Institute of Metrology, Division of Biological, Energy and Environment Measurement, Beijing, 100013, People's Republic of China. bosstrong@hotmail.com
Boqiang
B
Fu
Jing
J
Wang
Jean Michel
JM
Roturier
Zhiyu
Z
Tang
Huan
H
Li
Guangyan
G
Wei
D002681:China; D002853:Chromatography, Liquid / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D004043:Dietary Fiber / Q000032:analysis* / Q000592:standards; D005504:Food Analysis / Q000379:methods* / Q000592:standards / Q000706:statistics & numerical data; D011134:Polysaccharides / Q000032:analysis* / Q000592:standards; D012015:Reference Standards; D015203:Reproducibility of Results
D004043:Dietary Fiber; D011134:Polysaccharides; C008315:maltodextrin