Digital holography of total internal reflection.
We introduce a new microscopy technique termed total internal reflection holographic microscopy (TIRHM). Quantitative phase microscopy by digital holography is used to image the phase profile of light in total internal reflection, which is modulated by the materials present on or near the surface of internal reflection. The imaging characteristics are theoretically modeled and imaging capabilities are experimentally demonstrated.
Ash
William M
WM
Digital Holography and Microscopy Laboratory, Dept. of Physics, University of South Florida, Tampa, FL 33620, USA. wash@mail.usf.edu
Kim
Myung K
MK
D004867:Equipment Design; D019544:Equipment Failure Analysis; D006696:Holography / Q000295:instrumentation*; D007089:Image Enhancement / Q000295:instrumentation*; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation* / Q000379:methods*; D008857:Microscopy, Interference / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*