Development of a more efficient algorithm for identifying false-positive reactivity results in a dengue virus immunoglobulin M screening assay.
Of 2,692 sera screened for dengue virus immunoglobulin M by using a mu-capture enzyme-linked immunosorbent assay (ELISA), 954 had equivocal (index from 0.90 to 1.10) or positive (index of >1.10) results and were retested using a background subtraction (BS) ELISA that identifies screen false positives. No false positives were found among 427 sera with screen ELISA indices of >6.00; thus, retesting this specimen subset by BS ELISA is unnecessary.
Prince
Harry E
HE
Focus Diagnostics Inc., Cypress, California 90630, USA. hprince@focusdx.com
Yeh
Cindy
C
Lapé-Nixon
Mary
M
D000465:Algorithms; D000914:Antibodies, Viral / Q000097:blood; D003715:Dengue / Q000175:diagnosis* / Q000276:immunology; D003716:Dengue Virus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005189:False Positive Reactions; D006801:Humans; D007075:Immunoglobulin M / Q000097:blood*; D008403:Mass Screening / Q000379:methods*
D000914:Antibodies, Viral; D007075:Immunoglobulin M