Towards an integrated microfluidic device for spaceflight clinical diagnostics Microchip-based solid-phase extraction of hydroxyl radical markers.
A microchip-based solid-phase extraction method for biological fluid small molecule analysis has been developed. Using a commercially available copolymer packed into a microchip channel, extraction and preconcentration of 2,3-dihydroxybenzoic acid (DHBA) and 2,5-DHBA from saliva was achieved. The metabolites, formed from salicylic acid by reactive oxygen species, can be used as markers of oxidative stress. The results show high recovery of both metabolites (>90+/-15% for spiked saliva) with an 80-fold concentration enhancement possible. The eluent is directly analyzed using capillary electrophoresis, with good resolution for the two metabolites. This study demonstrates the feasibility of future integrated microdevices for spaceflight small molecule biomarker analysis.
Marchiarullo
Daniel J
DJ
Department of Chemistry, University of Virginia, Charlottesville, VA 22904, USA.
Lim
Ji Y
JY
Vaksman
Zalman
Z
Ferrance
Jerome P
JP
Putcha
Lakshmi
L
Landers
James P
JP
D062385:Hydroxybenzoates / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification; D017665:Hydroxyl Radical / Q000032:analysis* / Q000737:chemistry / Q000302:isolation & purification; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*; D013026:Space Flight
D062385:Hydroxybenzoates; D017665:Hydroxyl Radical