No evidence of involvement of Chlamydia pneumoniae in lung cancer by means of quantitative real-time polymerase chain reaction.
Chlamydia pneumoniae, an obligate intracellular pathogen, is well-known as etiological agent of acute respiratory infections; the repeated or prolonged exposure to chlamydial antigens may promote the persistence of C. pneumoniae in the respiratory tract leading to chronic diseases, such as chronic obstructive pulmonary disease and asthma. The predilection of C. pneumoniae to cause respiratory tract infections combined with its persistent nature suggest that it might play a role in lung cancer. The aim of our study is to evaluate the involvement of C. pneumoniae in pathogenesis of lung cancer. We therefore investigated the presence of C. pneumoniae DNA in tumor lung tissues by using real-time PCR assay. Simultaneously, tumor and healthy tissues from the same patient with primary carcinoma lung were analyzed. C. pneumoniae DNA was not detected in a single lung tumor tissue by means of an highly sensitive, and specific real-time PCR assay based on FRET hybridization probes. In conclusion, this study does not support the involvement of C. pneumoniae in the pathogenesis of lung cancer, suggesting that further investigations are needed to clarify other potential causative factors for the development of this malignancy.
Sessa
R
R
Department of Public Health Sciences, Sapienza University Rome, Piazzale Aldo Moro 5, Rome, Italy. rosa.sessa@uniroma1.it
Santino
I
I
Di Pietro
M
M
Schiavoni
G
G
Ripa
C
C
Galdiero
M
M
Iannone
M
M
Izzo
L
L
Mingazzini
P I
PI
Bolognese
A
A
Del Piano
M
M
D000368:Aged; D016993:Chlamydophila pneumoniae / Q000235:genetics* / Q000502:physiology; D004269:DNA, Bacterial / Q000235:genetics* / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000382:microbiology* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D010957:Plasmids / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
D004269:DNA, Bacterial