Emergence of CTX-M-15 type extended-spectrum beta-lactamase-producing Salmonella spp. in Kuwait and the United Arab Emirates.
Cephalosporins are major antimicrobials used to treat serious Salmonella infections. However, their effectiveness is being compromised by the emergence of extended-spectrum beta-lactamases (ESBLs). The genetic determinants encoding ESBL in Salmonella spp. isolated from patients in Kuwait and United Arab Emirates (UAE) were studied over a 2 year period. Out of a total of 407 isolates, 116 isolates possessed the resistance phenotypes consistent with possible ESBL production. Of these, 69 (59.5 %) were ESBL positive. PCR and sequencing were used to determine the genetic determinant(s) responsible for ESBL phenotypes. A total of 14 (12.1 %) and 29 (24.6 %) isolates were CTX-M-15 ESBL producers and TEM producers, respectively. Ten CTX-M-15 producers carried the insertion sequence ISEcpI gene. PFGE analysis revealed identical profiles in 4 of the 13 Kuwaiti strains. This study reports the presence of the bla(CTX-M-15) gene in Salmonella spp. and Salmonella enterica serotype Typhi from Kuwait and UAE for what is believed to be the first time. This is of great concern as the gene is also found in association with the ISEcpI gene, which may easily facilitate its spread. These isolates originated mostly from non-Kuwaiti Arabs rather than from people of Asian origin.
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Vincent O
VO
Rotimi
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
Wafaa
W
Jamal
Department of Microbiology & Immunology, Pecs University, Pecs, Hungary.
Tibor
T
Pal
Department of Microbiology & Immunology, Pecs University, Pecs, Hungary.
Agnes
A
Sovenned
Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait.
M John
MJ
Albert
D016521:Electrophoresis, Gel, Pulsed-Field; D006801:Humans; D007730:Kuwait / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D012475:Salmonella / Q000145:classification* / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D012480:Salmonella Infections / Q000453:epidemiology / Q000382:microbiology; D012485:Salmonella typhi / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D017422:Sequence Analysis, DNA; D014435:Typhoid Fever / Q000453:epidemiology / Q000382:microbiology; D014479:United Arab Emirates / Q000453:epidemiology; D018440:beta-Lactam Resistance; D001618:beta-Lactamases / Q000096:biosynthesis* / Q000235:genetics; D047090:beta-Lactams / Q000494:pharmacology
D047090:beta-Lactams; C473109:beta-lactamase CTX-M-15; D001618:beta-Lactamases