Comparison of Vasosol and University of Wisconsin solutions on early kidney function after 24 hours of cold ischemia in a canine autotransplantation model.
BACKGROUND
Ischemia/reperfusion (I/R) injury is a significant cause of graft dysfunction in donor kidney transplantation. It has been suggested that improvements in organ preservation solutions can ameliorate some of deleterious effects of I/R on the transplanted graft. We evaluated herein the influence of Vasosol (VAS), a solution that is designed to target specific pathways of I/R injury, and University of Wisconsin (UW) solution on early graft status of donor kidneys in a canine autotransplant model.


METHODS
Left kidneys were recovered from 12 dogs, exsanguinated with either VAS or UW and cooled to 4 degrees C for 24 h. Kidneys were autotransplanted and the right kidneys were nephrectomized. Indices of post-transplant renal function were measured serially for seven days. All animals were euthanized at postoperative day 7. Kidney biopsies were taken at 1, 4, and 24 h postreperfusion for evaluation of tissue myeloperoxidase concentration.


RESULTS
All dogs survived the transplant surgery. Post-transplant serum creatinine (mg/dL) and blood urea nitrogen (mg/dL) were significantly elevated in the UW group compared with the VAS group in each of the postoperative days. Moreover, myeloperoxidase tissue levels were significantly elevated in the UW-treated group compared with the VAS-treated group.


CONCLUSIONS
Our data suggest that a cold storage preserving solution designed to target several modes of I/R injury can improve the function of the autotransplanted canine kidney compared with the current gold standard solution.
Shands Transplant Center, University of Florida, Gainesville, Florida, USA. maxp@ufl.edu
Maximilian M R
MM
Polyak
Astrid
A
Grosche
D000241:Adenosine / Q000494:pharmacology; D000493:Allopurinol / Q000494:pharmacology; D000818:Animals; D001806:Blood Urea Nitrogen; D050377:Cold Ischemia; D003404:Creatinine / Q000097:blood; D004285:Dogs; D005978:Glutathione / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology; D007668:Kidney / Q000187:drug effects* / Q000201:enzymology; D016030:Kidney Transplantation; D009926:Organ Preservation; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D009195:Peroxidase / Q000378:metabolism; D011887:Raffinose / Q000494:pharmacology; D014182:Transplantation, Autologous
D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D003404:Creatinine; D009195:Peroxidase; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose