TATA-binding protein recognition and bending of a consensus promoter are protein species dependent.
The structure and behavior of full-length human TBP binding the adenovirus major late promoter (AdMLP) have been characterized using biophysical methods. The human protein induces a 97 degrees bend in DNA AdMLP. The high-resolution functional data provide a quantitative energetic and kinetic description of the partial reaction sequence as native human TBP binds rapidly to a consensus promoter with high affinity. The reaction proceeds with successive formation of three bound species, all having strongly bent DNA, with the concurrence of binding and bending demonstrated by both fluorescence and anisotropy stopped flow. These results establish the protein species dependence of the TBP-DNA AdMLP structure and recognition mechanism. Additionally, the strong correlation between the DNA bend angle and transcription efficiency demonstrated previously for yeast TBP is shown to extend to human TBP. The heterologous NH 2-terminal domains are the apparent source of the species-specific differences. Together with previous studies the present work establishes that TBP wt-DNA TATA function and structure depend both on the TATA box sequence and on the TBP species.
Whittington
JoDell E
JE
Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska 68588-0304, USA.
Delgadillo
Roberto F
RF
Attebury
Torrissa J
TJ
Parkhurst
Laura K
LK
Daugherty
Margaret A
MA
Parkhurst
Lawrence J
LJ
D000256:Adenoviridae / Q000235:genetics; D016384:Consensus Sequence; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D005453:Fluorescence; D006801:Humans; D007700:Kinetics; D009690:Nucleic Acid Conformation; D011401:Promoter Regions, Genetic / Q000235:genetics*; D011485:Protein Binding; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012996:Solutions; D013045:Species Specificity; D035181:TATA-Box Binding Protein / Q000737:chemistry / Q000378:metabolism*; D013696:Temperature; D013816:Thermodynamics; D014158:Transcription, Genetic
D012996:Solutions; D035181:TATA-Box Binding Protein; D004247:DNA