Bactericidal and biocompatible properties of TiN/Ag multilayered films by ion beam assisted deposition.
Nanoscale TiN/Ag multilayered films of thickness 500 nm were synthesized on AISI317 stainless steel by ion beam assisted deposition (IBAD) with the modulation period of 4, 5, 6, 7.5, and 12 nm. The bactericidal and biocompatible properties of TiN/Ag multilayered films were investigated through Gram negative E. coli bacteria and L929 cells (mice fibroblast) as well as human umbilical vein endothelial cells (HUVEC). The results show that the TiN/Ag multilayered films with the modulation period of 7.5 nm possess the strongest bactericidal property. The cytotoxicity grade of TiN/Ag multilayered coating with the modulation periods of 7.5 nm, 12 nm is in 0-1 scope, which indicates this film has no cytotoxicity to L929. HUVEC on TiN/Ag multilayered film grows well and shows good cellularity. Auger electronic spectroscopy reveals the relationship between the structure of TiN/Ag multilayered film and the biomedical properties.
College of Physics and Electronic Information Science, Tianjin Normal University, Tianjin 300387, People's Republic of China. jiezhao1943@126.com
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D000497:Alloys / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004595:Electroplating / Q000379:methods*; D042783:Endothelial Cells / Q000187:drug effects / Q000502:physiology; D004926:Escherichia coli / Q000187:drug effects / Q000254:growth & development / Q000502:physiology; D006801:Humans; D008422:Materials Testing; D008567:Membranes, Artificial; D008826:Microbial Sensitivity Tests; D012834:Silver / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
D000497:Alloys; D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D008567:Membranes, Artificial; D012834:Silver; C041500:titanium nitride; D014025:Titanium