Use of chemically modified activated carbon as a support for immobilized enzymes.
Loading and activity assays of the enzymes alpha-chymotrypsin, alpha-chymotrypsinogen, and glucose oxidase covalently bound to an activated carbon support are presented. The activated carbon support material was pretreated using either a radio-frequency oxygen plasma or an electrochemical oxidation to maximize the enzyme attachment. Cyanuric chloride or water-soluble carbodiimide linking reactions were used to covalently attach the enzymes to the carbon support. Discussion of the relative merits of each reaction scheme is presented.
Department of Chemistry, Rutgers University, New Brunswick, New Jersey 08903.
J A
JA
Osborn
R M
RM
Ianniello
H J
HJ
Wieck
T F
TF
Decker
S L
SL
Gordan
A M
AM
Yacynych