In vitro activity of Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole against different dermatophytes.
Strains of five dermatophyte species (Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum and Trichophyton tonsurans) were selected for testing against Penicillium chrysogenum antifungal protein (PAF) and its combination with fluconazole (FCZ). Inhibition of microconidia germination and growth was detected with MICs of PAF ranging from 1.56 to 200 microg ml(-1) when it was used alone, or at constant concentration (100 microg ml(-1)) in combination with FCZ at from 0.25 to 32 microg ml(-1). The MICs for FCZ were found to be between 0.25 and 128 microg ml(-1). PAF caused a fungicidal effect at 200 microg ml(-1) and reduced growth at between 50 and 200 microg ml(-1). Total growth inhibition with fungistatic activity was detected at 64 microg ml(-1) of FCZ for M. gypseum, T. mentagrophytes, and T. tonsurans, and at 32 microg ml(-1) FCZ for M. canis and T. rubrum. PAF and FCZ acted synergistically and/or additively on all of the tested fungi except M. gypseum, where no interactions were detected.
Department of Microbiology, Faculty of Science and Informatics, University of Szeged, Közép fasor 52., Szeged, Hungary. galgoczi@gmail.com
László
L
Galgóczy
Tamás
T
Papp
István
I
Pócsi
Nikoletta
N
Hegedus
Csaba
C
Vágvölgyi
D000818:Animals; D000935:Antifungal Agents / Q000008:administration & dosage*; D003883:Arthrodermataceae / Q000187:drug effects* / Q000472:pathogenicity; D003881:Dermatomycoses / Q000188:drug therapy; D025141:Drug Resistance, Fungal; D004357:Drug Synergism; D015725:Fluconazole / Q000008:administration & dosage*; D005656:Fungal Proteins / Q000008:administration & dosage* / Q000502:physiology*; D006801:Humans; D008864:Microsporum / Q000187:drug effects; D010408:Penicillium chrysogenum / Q000502:physiology*; D014249:Trichophyton / Q000187:drug effects
D000935:Antifungal Agents; D005656:Fungal Proteins; C097832:PAF protein, Penicillium chrysogenum; D015725:Fluconazole