Reaction of rat connective tissue to a new calcium hydroxide-based sealer.
The aim of this study was to histopathologically examine the reaction of the connective tissue of rats to 2 calcium hydroxide-based sealers, Acroseal and Sealapex. Dentin tubes containing the materials and empty control tubes were implanted into the dorsal connective tissue of 36 Wistar albino rats. The animals were killed after 7 or 30 days, and the specimens were prepared for histologic analysis with hematoxylin and eosin, Von Kossa technique, and polarized light. Results were statistically analyzed using Kruskal-Wallis test. Both materials caused mild or moderate inflammatory reactions on the 7th day, but these reactions decreased by the 30th day with no significant difference at any time (P > .05). Mineralization of the subcutaneous tissue of the rats was observed only with Sealapex.
Department of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, Brazil. joao@foa.unesp.br
João Eduardo
JE
Gomes-Filho
Pedro Felício Estrada
PF
Bernabé
Mauro Juvenal
MJ
Nery
José Arlindo
JA
Otoboni-Filho
Eloi
E
Dezan-Júnior
Mariana Machado Teixeira
MM
de Moraes Costa
Max Douglas
MD
de Faria
Simone
S
Watanabe
Alessandra Cristina
AC
Gomes
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002126:Calcium Hydroxide / Q000737:chemistry*; D003238:Connective Tissue / Q000473:pathology; D003804:Dentin / Q000473:pathology; D003872:Dermatitis / Q000473:pathology; D006801:Humans; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D008297:Male; D008422:Materials Testing; D009336:Necrosis; D009504:Neutrophils / Q000473:pathology; D009999:Ossification, Heterotopic / Q000139:chemically induced; D010950:Plasma Cells / Q000473:pathology; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials / Q000737:chemistry*; D012459:Salicylates / Q000737:chemistry; D012867:Skin / Q000473:pathology*; D013997:Time Factors
C477053:Acroseal; D001672:Biocompatible Materials; D012387:Root Canal Filling Materials; D012459:Salicylates; C055601:Sealapex; D002126:Calcium Hydroxide