Electrophoretic study of the macromolecular compounds excreted by yeasts: application to differentiation between strains of the same species.
The macromolecular compounds excreted during growth by yeasts as exocellular fractions were studied and found to contain a high proportion of carbohydrates. On the basis of the specificity of these fractions, an attempt was made to distinguish between nine strains of a single species of yeast-Saccharomyces cerevisiae. Each strain came from a different source. The electrophoretic patterns of the exocellular fractions tested made it possible to distinguish between the nine strains, on the basis of Lodder's criteria for the morphological and physiological identification of yeasts. We considered that strains giving exocellular fractions distinguishable by electrophoresis belonged to distinct clones. Consequently, this technique enables us to go further than diagnosis of the species.
Chaire de Microbiogie Appliquée et Industries Microbiennes, Département de Biotechnologie, Ecole Nationale Supérieure des Industries Agricoles et Alimentaires, 105 rue d l'Université, 59509 Douai, France.
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