Anti-inflammatory and anti-tumour effects of Abies georgei extracts.
Chloroform (AGC), ethyl acetate (AGE) and n-butanol (AGB) extracts of Abies georgei were investigated for anti-tumour and anti-inflammatory activities in-vitro and in-vivo. AGC exhibited potent antiproliferative effects against A549, LOVO, QGY-7703 and 6T-CEM tumour cells, with EC50 values of 77.5, 7.8, 11.1 and 32.8 microg mL(-1), respectively. It also inhibited the growth of S180 sarcoma implanted into mice; tumour growth inhibition ratios were 46.7, 53.1 and 31.0% of controls at doses of 100, 200 and 400 mgkg(-1), respectively. AGE showed significant anti-inflammatory activities in the carrageenin-induced acute pedal oedema model in rats and dimethylbenzene-induced ear oedema in mice at doses of 140 mgkg(-1) and 200 mgkg(-1) p.o., respectively. Primary mechanism studies in-vitro showed that AGE inhibited platelet aggregation induced in rabbits by arachidonic acid (AA), with an IC50 of 14.4 microg mL(-1). Its effect on AA metabolism was also studied in mouse peritoneal macrophages stimulated by A23187. Formation of prostaglandin E(2), leukotriene B(4) and 5S-hydroxy-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-HETE) was significantly inhibited in a concentration-dependent manner. In addition, AGE inhibited lipopolysaccharide-induced nitric oxide production in RAW246.7 macrophages and nuclear factor kappaB activation induced in 293 cells by tumour necrosis factor alpha.
Yang
Xian-Wen
XW
Department of Natural Product Chemistry, School of Pharmacy, Second Military Medical University, 325 Guohe Road, Shanghai 200433, PR China.
Zeng
Hua-Wu
HW
Liu
Xiao-Hua
XH
Li
Su-Mei
SM
Xu
Wen
W
Shen
Yun-Heng
YH
Zhang
Chuan
C
Zhang
Wei-Dong
WD
D028202:Abies / Q000737:chemistry*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002460:Cell Line; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000096:biosynthesis; D010936:Plant Extracts / Q000494:pharmacology*; D010974:Platelet Aggregation / Q000187:drug effects; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley
D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D016861:Cyclooxygenase Inhibitors; D010936:Plant Extracts; D009569:Nitric Oxide