Diversity and stability study on rice mutants induced in space environment.
To further study the characteristics of changes on the molecular level of rice mutants induced in space environment, we analyzed proteins in leaves and seeds of four rice mutants (two high-tillering and two low-tillering) in the 8(th) and 9(th) generations after a 15-day spaceflight, and compared with their ground controls by two-dimentional polyacrylamide gel electrophoresis and reverse phase liquid chromatography (RPLC). In addition, the albumin, globulin, prolamine, glutelin, and amylose of the mutant seeds were analyzed by RPLC and ultra-violet spectrometry. The results showed that the low-abundance proteins of leaves in the peak tillering stage are more likely to be induced compared with their corresponding controls. The albumin, globulin, and prolamine of the mutant seeds revealed changes when compared with their controls, and the characteristics of changes in different mutants were stably inherited in the 8(th) and 9(th) generations, suggesting that they can be used as bio markers to identity the mutants induced by spaceflight. Moreover, two proteins (SSP9111 and SSP6302) were found to be expressed with high intensity (two-fold change) in different mutants, which were both correlated with photosystem according to mass spectrometry and database searching.
Department of Astronautic Engineering, Harbin Institute of Technology, Harbin 150001, China.
Wei-Hong
WH
Lu
Xin-Zhu
XZ
Wang
Qi
Q
Zheng
Shuang-Hong
SH
Guan
Ping
P
Xin
Ye-Qing
YQ
Sun
D000418:Albumins / Q000235:genetics / Q000378:metabolism; D000688:Amylose / Q000235:genetics / Q000378:metabolism; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D005118:Extraterrestrial Environment; D018506:Gene Expression Regulation, Plant; D014644:Genetic Variation; D042822:Genomic Instability; D005916:Globulins / Q000235:genetics / Q000378:metabolism; D013058:Mass Spectrometry; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000378:metabolism; D018515:Plant Leaves / Q000235:genetics; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D055315:Prolamins; D012639:Seeds / Q000235:genetics; D013026:Space Flight
D000418:Albumins; D005916:Globulins; D010940:Plant Proteins; D055315:Prolamins; D000688:Amylose