Immunohistochemical evaluation of 95 bone marrow reactive plasmacytoses.
We histologically and immunohistochemically studied 95 bone marrow (BM) reactive plasmacytoses. Ten biopsies from plasma cell myeloma (PCM) patients served as a control group. In addition, we studied 10 monoclonal gammopathy of undetermined significance (MGUS) cases. Histologically, plasmacytosis varied between 5% and 25% with an interstitial pattern of plasma cell (PC) distribution being characteristically displayed. Immunohistochemically, we did not find any CD56/NCAM nor cyclin D1 expression in all biopsies (95 of 95, 100%), not even a weak, doubtful one; PCs were all polyclonal and CD138 positive. On the contrary, myeloma-associated PCs showed monoclonality for kappa- or lambda- light chain and strong CD56/NCAM immunoreactivity (8 of 10, 80%); four of them were cyclin D1 positive. Osteoblasts exhibited similar CD56/NCAM expression in both groups. Our data confirm the diagnostic utility of CD56/NCAM in the phenotypic characterization of polyclonal plasma cells, suggesting an important role of this particular immunomarker in the BM trephine study of polyclonal versus neoplastic plasmacytic infiltrations.
Department of Pathology, Medical School, University of Thessaly, Larisa, Greece.
Maria G
MG
Ioannou
Efstathios
E
Stathakis
Andreas C
AC
Lazaris
Thomas
T
Papathomas
Evangelos
E
Tsiambas
George K
GK
Koukoulis
D001853:Bone Marrow / Q000378:metabolism* / Q000473:pathology; D019002:CD56 Antigen / Q000378:metabolism*; D019938:Cyclin D1 / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D007146:Immunoglobulin lambda-Chains / Q000378:metabolism*; D009101:Multiple Myeloma / Q000378:metabolism* / Q000473:pathology; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism*; D010950:Plasma Cells / Q000378:metabolism* / Q000473:pathology; D012189:Retrospective Studies
C530104:CCND1 protein, human; D019002:CD56 Antigen; D007146:Immunoglobulin lambda-Chains; D019006:Neural Cell Adhesion Molecules; D019938:Cyclin D1