Antiproliferative effect of synthetic lignin against human breast cancer and normal fetal lung cell lines. Potency of low molecular weight fractions.
OBJECTIVE
Due to a lack of chemotherapeutics to efficiently control neoplastic processes, there is a need for discovering new, more efficient anticancer drugs that would distinguish malignant from normal cells.


METHODS
We studied the effect of short (4 h) and long (72 h) treatment with different concentrations of the enzymatically synthesized lignin model compound (DHP) on the proliferation of two human cell lines grown in tissue culture: breast adenocarcinoma (MCF7) and normal fetal lung fibroblast (MRC5) cell lines.


RESULTS
The growth of both MRC5 and MCF7 cell lines was inhibited by DHP after 4 h-treatment, while the carcinoma cell line was also sensitive to the long-term treatment with lower dose of DHP in comparison with the fetal cells. The low molecular weight DHP fractions inhibited growth of the MRC5 cells at lower concentrations compared to the treatment with all DHP fractions.


CONCLUSIONS
The higher sensitivity to DHP of the human malignant cells compared to the normal transformed ones gives the possibility to further study DHP as a therapeutic agent.
Andrijevic
Lj
Lj
Institute of Oncology Sremska Kamenica, Sremska Kamenica, Serbia.
Radotic
K
K
Bogdanovic
J
J
Mutavdzic
D
D
Bogdanovic
G
G
D000230:Adenocarcinoma / Q000188:drug therapy / Q000473:pathology; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005333:Fetus / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D006801:Humans; D008031:Lignin / Q000138:chemical synthesis / Q000627:therapeutic use*; D008168:Lung / Q000166:cytology / Q000187:drug effects*; D008970:Molecular Weight
D008031:Lignin