In vitro RNA synthesis by intact rat brain nuclei.
The characteristics of DNA-dependent RNA polymerase activity in intact rat cerebral cortex nuclei tested at low ionic strength are presented. The system was most dependent on the presence of spermidine and an ATP-generating system and to a lesser extent on Mg2+ and K+ for maximal incorporation. Substitution of Mg2+ by Mn2+ or of K+ by Na+ resulted in substantially less activity than under the optimum conditions described. Maximal incorporation was about 10 per cent that of brain nuclear systems of high ionic strength. In addition, the labelling patterns of the in vitro RNA products were shown to be very similar to those found in vivo. The stability of isolated nuclei toward degradation of RNA synthetic capacity and products formed was much greater than that of a similar liver system.
Dutton
G R
GR
Chemical Laboratories, Indiana University Bloomington, Indiana 47401, USA.
Mahler
H R
HR
D000255:Adenosine Triphosphate / Q000494:pharmacology; D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism*; D002540:Cerebral Cortex / Q000648:ultrastructure*; D004247:DNA / Q000378:metabolism; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D066298:In Vitro Techniques; D008297:Male; D009711:Nucleotides / Q000378:metabolism; D012313:RNA / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013095:Spermidine / Q000494:pharmacology
D009711:Nucleotides; D012313:RNA; D000255:Adenosine Triphosphate; D004247:DNA; D012321:DNA-Directed RNA Polymerases; D013095:Spermidine