Characterization and promoter activity of chromoplast specific carotenoid associated gene (CHRC) from Oncidium Gower Ramsey.
Tissue-specific promoters are required for plant molecular breeding to drive a target gene in the appropriate location in plants. A chromoplast-specific, carotenoid-associated gene (OgCHRC) and its promoter (Pchrc) were isolated from Oncidium orchid and characterized. Northern blot analysis revealed that OgCHRC is specifically expressed in flowers, not in roots and leaves. Transient expression assay of Pchrc by bombardment transformation confirmed its differential expression pattern in floral tissues of different horticulture plants and cell-type location in conical papillate cells of adaxial epidermis of flower. These results suggest that Pchrc could serve as a useful tool in ornamental plant biotechnology to modify flower color.
Chiou
Chung-Yi
CY
Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei 106, Taiwan.
Wu
Keqiang
K
Yeh
Kai-Wun
KW
D001483:Base Sequence; D019470:Biolistics; D015152:Blotting, Northern; D002338:Carotenoids / Q000378:metabolism*; D017931:DNA Primers; D018744:DNA, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D029595:Orchidaceae / Q000235:genetics*; D011401:Promoter Regions, Genetic
D017931:DNA Primers; D018744:DNA, Plant; D002338:Carotenoids