Isolation and characterization of plasma membrane Na(+)/H(+) antiporter genes from salt-sensitive and salt-tolerant reed plants.
We isolated cDNAs for Na(+)/H(+) antiporter genes (PhaNHA1s) from salt-sensitive and salt-tolerant reed plants. A phylogenetic analysis and localization analysis using yeast strains expressing PhaNHA1-GFP protein showed that PhaNHA1s were plasma membrane Na(+)/H(+) antiporters. Yeast strains expressing PhaNHA1 from salt-tolerant reed plants (PhaNHA1-n) grew well than yeast strains expressing PhaNHA1 from salt-sensitive reed plants (PhaNHA1-u) in the presence of 100mM NaCl. Furthermore, Na(+) contents of yeast cells expressing PhaNHA1-n were less than half of those of yeast cells expressing PhaNHA1-u. These results suggest that PhaNHA1-n is more efficient at excluding Na(+) from the cells than PhaNHA1-u.
Asian Natural Environmental Science Center (ANESC), The University of Tokyo, 1-1-1, Midori-cho, Nishitokyo-shi, Tokyo 188-0002, Japan.
Ryuichi
R
Takahashi
Shenkui
S
Liu
Tetsuo
T
Takano
D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000187:drug effects / Q000235:genetics*; D018076:DNA, Complementary / Q000302:isolation & purification; D018744:DNA, Plant / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D010802:Phylogeny; D006109:Poaceae / Q000166:cytology / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D021381:Protein Transport / Q000187:drug effects; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000254:growth & development; D055051:Salt-Tolerant Plants / Q000166:cytology / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D016415:Sequence Alignment; D012964:Sodium / Q000378:metabolism; D012965:Sodium Chloride / Q000494:pharmacology*; D017923:Sodium-Hydrogen Exchangers / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification* / Q000378:metabolism; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014170:Transformation, Genetic / Q000187:drug effects
D018076:DNA, Complementary; D018744:DNA, Plant; D011993:Recombinant Fusion Proteins; D017923:Sodium-Hydrogen Exchangers; D049452:Green Fluorescent Proteins; D012965:Sodium Chloride; D012964:Sodium