Microbial transformation of cinobufagin by Syncephalastrum racemosum.
Microbial transformation of a cytotoxic bufadienolide, cinobufagin (1), was performed by Syncephalastrum racemosum. The six metabolites obtained were identified as 7beta-hydroxycinobufagin ( 2), 12beta-hydroxycinobufagin (3), cinobufotalin (4), 5,12beta-dihydroxycinobufagin (5), 4beta,11alpha-dihydroxycinobufagin (6), and 4beta,12alpha-dihydroxycinobufagin (7), respectively, on the basis of spectroscopic studies. Metabolites 2 and 5-7 were characterized as new compounds, and 2-7 proved to be cytotoxic against Bel-7402 human hepatoma cells.
The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100083, People's Republic of China.
Xiao-chi
XC
Ma
Xiu-lan
XL
Xin
Ke-xin
KX
Liu
Jian
J
Han
De-an
DA
Guo
D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001711:Biotransformation; D002018:Bufanolides / Q000737:chemistry / Q000378:metabolism*; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D009090:Mucorales / Q000378:metabolism*; D019906:Nuclear Magnetic Resonance, Biomolecular
D000970:Antineoplastic Agents; D002018:Bufanolides; C087925:bufadienolide; C063451:cinobufotalin; C002471:cinobufagin