Primary isolation and detection of Helicobacter pylori from dyspeptic patients: a simple, rapid method.
The study aimed to develop a rapid and simple method for the primary isolation and detection of Helicobacter pylori from dyspeptic patients. Mucosal antral biopsy specimens were obtained from 136 consecutive dyspeptic patients diagnosed with peptic ulcer by endoscopy at Basra General Hospital, Iraq. From histopathological examination of biopsies, H. pylori was detected in 81 (59.6%) peptic ulcer patients. For bacterial culture, specimens were cultured in parallel on 2 media: the non-selective classic Columbia agar and the selective modified Columbia urea agar (MCUA). MCUA showed a higher isolation rate than classic Columbia agar (67.6% versus 44.1% of patients), and the results were obtained faster (24 hours versus 5-7 days) with more clear-cut identification.
Department of Biology, College of Education, University of Basra, Basra, Iraq. Aminabdulah@yahoo.com
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Al-Sulami
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Al-Kiat
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Bakker
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Hunoon
D000293:Adolescent; D000328:Adult; D000362:Agar; D000368:Aged; D001431:Bacteriological Techniques / Q000379:methods*; D001706:Biopsy / Q000379:methods*; D015169:Colony Count, Microbial; D003470:Culture Media; D004415:Dyspepsia / Q000382:microbiology*; D005260:Female; D005756:Gastritis / Q000382:microbiology*; D005773:Gastroscopy; D016481:Helicobacter Infections / Q000150:complications / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D007493:Iraq; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000382:microbiology*; D012720:Severity of Illness Index; D013997:Time Factors
D003470:Culture Media; D000362:Agar