A simple, sensitive, and accurate alcohol electrode.
The construction and performance of an enzyme electrode is described which specifically detects lower primary aliphatic alcohols in aqueous solutions. The electrode consists of a commercial Clark-type oxygen electrode on which alcohol oxidase (E.C. 1.1.3.13) and catalase were immobilized. The decrease in electrode current is linearly proportional to ethanol concentrations between 1 and 25 ppm. The response of the electrode remains constant during 400 assays over a period of two weeks. The response time is between 1 and 25 min. Assembly of the electrode takes less than 1 h.
Verduyn
C
C
Delft University of Technology, Laboratory of Microbiology, Julianalaan 67a, 2628 BC Delft, The Netherlands.
Van Dijken
J P
JP
Scheffers
W A
WA