Engineering motility as a phenotypic response to LuxI/R-dependent quorum sensing in Escherichia coli.
The repertoire of functional outputs interfaced with the LuxI/LuxR quorum sensing system in engineered Escherichia coli has been expanded to include motility via inducible expression of motB. Appropriate choice of ribosome binding site controlling MotB translation was crucial to achieving control over motility.
Lucien E
LE
Weiss
Jonathan P
JP
Badalamenti
Lane J
LJ
Weaver
Anthony R
AR
Tascone
Paul S
PS
Weiss
Tom L
TL
Richard
Patrick C
PC
Cirino
D054742:Acyl-Butyrolactones / Q000378:metabolism; D001426:Bacterial Proteins / Q000096:biosynthesis* / Q000235:genetics* / Q000378:metabolism*; D001709:Biotechnology / Q000379:methods; D002633:Chemotaxis / Q000235:genetics*; D004790:Enzyme Induction; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D009876:Operon; D011401:Promoter Regions, Genetic / Q000502:physiology; D053038:Quorum Sensing / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D015534:Trans-Activators / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
D054742:Acyl-Butyrolactones; D001426:Bacterial Proteins; D029968:Escherichia coli Proteins; C085427:LuxI protein, Bacteria; C064779:MotB protein, Bacteria; D012097:Repressor Proteins; D015534:Trans-Activators; D014157:Transcription Factors; C064778:LuxR autoinducer binding proteins