Suppression of histamine signaling by probiotic Lac-B: a possible mechanism of its anti-allergic effect.
It has been shown that probiotic bacteria are effective for the treatment of allergic diseases. As histamine plays a central role in allergic diseases, it is possible that probiotic bacteria affect the allergy-related histamine signaling. Here, we investigated the effect of Lac-B, a mixture of freeze-dried Bifidobacterium infantis and Bifidobacterium longum, on the allergy-related histamine signaling. In the nasal allergy model rats made by sensitization and provocation with toluene 2,4-diisocyanate (TDI) for 3 weeks, TDI provocation caused acute allergy-like behaviors along with significant up-regulation of histamine H(1) receptor (H1R) and histidine decarboxylase (HDC) mRNA expression, increased HDC activity, histamine content, and [(3)H]mepyramine binding activity in nasal mucosa. Prolonged treatment with Lac-B (40 mg/rat, p.o.) significantly suppressed both the allergy-like behaviors and all of the above mentioned factors involved in histamine signaling. Our findings indicate that oral administration of Lac-B showed significant anti-allergic effect through suppression of both H1R and HDC gene expression followed by decrease in H1R, HDC protein level, and histamine content. Suppression of histamine signaling may be a novel target of probiotics in preventing allergic diseases.
Department of Molecular Pharmacology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
Shrabanti
S
Dev
Hiroyuki
H
Mizuguchi
Asish K
AK
Das
Chiyo
C
Matsushita
Kazutaka
K
Maeyama
Hayato
H
Umehara
Takayuki
T
Ohtoshi
Jun
J
Kojima
Kiyotaka
K
Nishida
Kunihiko
K
Takahashi
Hiroyuki
H
Fukui
D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology*; D016207:Cytokines / Q000235:genetics; D006633:Histamine Antagonists / Q000494:pharmacology*; D006640:Histidine Decarboxylase / Q000235:genetics; D008297:Male; D019936:Probiotics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000032:analysis; D051381:Rats; D011914:Rats, Inbred BN; D011969:Receptors, Histamine H1 / Q000235:genetics; D015398:Signal Transduction / Q000187:drug effects*; D014051:Toluene 2,4-Diisocyanate / Q000494:pharmacology
D018926:Anti-Allergic Agents; D016207:Cytokines; D006633:Histamine Antagonists; D012333:RNA, Messenger; D011969:Receptors, Histamine H1; D014051:Toluene 2,4-Diisocyanate; D006640:Histidine Decarboxylase