[Ultrastructural and functional assessment on platelets loaded with small molecule carbohydrates].
The aim of this study was to investigate the effect of loading some small molecule carbohydrates into human platelets on ultrastucture and function. The ultrastructure of platelets were observed by transmission electron microscope (TEM); the platelet counts and mean platelet volume (MPV) were measured by hemocytometer, the maximal platelet aggregation rate was measured optically in an aggregometer; the surface marker of platelet membranes CD62p and phosphatidyl serine were analyzed by flow cytometry. The results showed that no significant changes of the ultrastructure of platelets loaded with small molecule carbohydrates were seen. The aggregation responsiveness of platelets loaded with small molecule carbohydrates reached to 60% of the fresh control platelets. The values of platelet counts and MPV showed no significant differences. The expression level of CD62p and the binding rate with Annexin V before and after loading small molecule carbohydrates into platelets were no different. It is concluded that the platelets after loading with small molecule carbohydrates remained fine ultrastructure and function.
Beijing Institute of Transfusion Medicine, Beijing 100850, China.
Chao
C
Yang
Jie-Xi
JX
Wang
Ying
Y
Han
Yan
Y
Wang
Guo-Bo
GB
Quan
Min-Xia
MX
Liu
Feng
F
Gao
An
A
Liu
D001792:Blood Platelets / Q000502:physiology* / Q000648:ultrastructure*; D001793:Blood Preservation / Q000379:methods*; D002241:Carbohydrates / Q000494:pharmacology*; D005612:Freeze Drying; D006801:Humans; D019007:P-Selectin / Q000097:blood; D010974:Platelet Aggregation
D002241:Carbohydrates; D019007:P-Selectin