Preparation of human immune effector T cells containing iron-oxide nanoparticles.
Preparation of human immune T cells containing iron-oxide nanoparticles was carried out for the development of magnetically mediated immunotherapy. Peripheral blood lymphocytes (PBLs) after the incubation with magnetite nanoparticles were found to contain measurable ferric ions, which suggested the incorporation of magnetite nanoparticles. Transmission electron microscopic (TEM) study indicated that the incorporation of magnetite nanoparticles was mediated by endocytosis of PBLs. Furthermore, the effects of dosages and diameter of magnetite nanoparticles on the magnetite incorporation were investigated, and it was demonstrated that the increase in dosage promoted the incorporation of nanoparticles and the uptake into PBLs was more effective for magnetite nanoparticles, which formed smaller aggregations in medium. Finally, the demonstration of magnetite incorporation into enriched T cells and tumor antigen-specific cytotoxic T lymphocyte (CTL) line promises the achievement of magnetically mediated immunotherapy with tumor-specific CTLs containing magnetic nanoparticles.
Department of Applied Chemistry, School of Science and Engineering, Waseda University, Tokyo, Japan.
Hironori
H
Iida
Kosuke
K
Takayanagi
Takuya
T
Nakanishi
Akiko
A
Kume
Kouji
K
Muramatsu
Yoshio
Y
Kiyohara
Yasuto
Y
Akiyama
Tetsuya
T
Osaka
D002478:Cells, Cultured; D003593:Cytoplasm / Q000648:ultrastructure; D004705:Endocytosis; D005290:Ferric Compounds / Q000378:metabolism*; D052203:Ferrosoferric Oxide / Q000378:metabolism; D006801:Humans; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology* / Q000378:metabolism* / Q000648:ultrastructure
D005290:Ferric Compounds; C000499:ferric oxide; D052203:Ferrosoferric Oxide