Minor spliceosome components are predominantly localized in the nucleus.
Recently, it has been reported that there is a differential subcellular distribution of components of the minor U12-dependent and major U2-dependent spliceosome, and further that the minor spliceosome functions in the cytoplasm. To study the subcellular localization of the snRNA components of both the major and minor spliceosomes, we performed in situ hybridizations with mouse tissues and human cells. In both cases, all spliceosomal snRNAs were nearly exclusively detected in the nucleus, and the minor U11 and U12 snRNAs were further shown to colocalize with U4 and U2, respectively, in human cells. Additionally, we examined the distribution of several spliceosomal snRNAs and proteins in nuclear and cytoplasmic fractions isolated from human cells. These studies revealed an identical subcellular distribution of components of both the U12- and U2-dependent spliceosomes. Thus, our data, combined with several earlier publications, establish that, like the major spliceosome, components of the U12-dependent spliceosome are localized predominantly in the nucleus.
Institute of Biotechnology, PL 56 (Viikinkaari 9), and Institute of Biomedicine, Biomedicum Helsinki, PL 63 (Haartmaninkatu 8), University of Helsinki, FIN-00014, Helsinki, Finland.
Heli K J
HK
Pessa
Cindy L
CL
Will
Xiaojuan
X
Meng
Claudia
C
Schneider
Nicholas J
NJ
Watkins
Nina
N
Perälä
Mariann
M
Nymark
Janne J
JJ
Turunen
Reinhard
R
Lührmann
Mikko J
MJ
Frilander
D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D006367:HeLa Cells; D006801:Humans; D017403:In Situ Hybridization; D051379:Mice; D008856:Microscopy, Fluorescence; D012322:RNA Precursors / Q000378:metabolism; D012326:RNA Splicing; D012342:RNA, Small Nuclear / Q000032:analysis* / Q000378:metabolism; D017381:Spliceosomes / Q000378:metabolism*
D012322:RNA Precursors; D012342:RNA, Small Nuclear; C096986:U12 small nuclear RNA