An evaluation of bacterial contamination of barriers used in periapical tissue regeneration: Part 1--Bacterial adherence.
OBJECTIVE
To compare the adherence of Prevotella melaninogenica and Enterococcus faecalis to 3 guided tissue regeneration membranes: Atrisorb, Lambone, and OsseoQuest. It was hypothesized that OsseoQuest would show increased bacterial adherence compared to Lambone and Atrisorb.


METHODS
The barriers were suspended in trypticase soy broth containing an inoculum of either P melaninogenica or E faecalis. The samples were incubated under appropriate conditions for 6, 24, and 48 hours. Following incubation, each membrane was mixed in fresh media in a vortex machine to dislodge adherent bacteria. The vortexed media was quantitatively assessed using serial dilutions for viable cell count.


RESULTS
E faecalis exhibited higher adherence compared to P melaninogenica with time. Of the membranes tested, Lambone displayed the least bacterial adherence.


CONCLUSIONS
An analysis of the results indicated that bacterial adherence was time-dependent for all membranes. Membrane structure, chemical configuration, hydrophobicity, and bacterial cell surface structure were suggested as factors contributing to variance in bacterial adherence.
Sharma
Priya
P
Department of Endodontics, Prosthodontics, and Operative Dentistry, Baltimore College of Dentistry, University of Maryland, Baltimore, MD 21201, USA. PChand@umaryland.edu
Mickel
André K
AK
Chogle
Sami
S
Sharma
Prem Nath
PN
Han
Yiping W
YW
Jones
Jefferson J
JJ
D001422:Bacterial Adhesion; D001672:Biocompatible Materials / Q000737:chemistry*; D018786:Bone Substitutes / Q000737:chemistry; D015169:Colony Count, Microbial; D013293:Enterococcus faecalis / Q000502:physiology*; D016557:Guided Tissue Regeneration, Periodontal / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D019344:Lactic Acid / Q000737:chemistry; D008422:Materials Testing; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D001443:Prevotella melaninogenica / Q000502:physiology*; D013499:Surface Properties; D013997:Time Factors
D001672:Biocompatible Materials; D018786:Bone Substitutes; D008567:Membranes, Artificial; D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide); C046409:polyglyconate