[Influence of unilateral breaking of the uterus ligation of treatment agent on intrauterine injection of pregnant mice].
OBJECTIVE
To investigate the influence of unilateral breaking of the uterus ligation of treatment agent.


METHODS
The pregnant 9-18 day mice was randomly divided into two groups, the control group were used intrauterine bilateral embryonic amniotic (D10-D12) and abdominal (D14-D16) injection of saline 20 microL, the experimental group by ligation of unilateral breaking of the uterus, were used unilateral intrauterine embryo amniotic (D10-D12) and abdominal (D14-D16) injection of saline 20 microL. After surgery, the pregnancy after mice survived, childbirth, and their offspring of the mice developed indexes were observed.


RESULTS
In contrast with compared group, we found that half uterus deligation disjuction used intrauterine injection could decrease abortion ratio, unintentional mortality and still birth rate, meantime, improve the pregnant mice survival and the delivery rate (P < 0.05), and the development of offsprings was no distinguish (P > 0.05).


CONCLUSIONS
Half uterus deligation disjuction could elevate the effect on the study of intrauterine embryo in pregnant mice, and it is feasible.
Department of Histology and Embryology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Fang
F
Ma
Yuan
Y
Wang
Jie
J
Zhao
D000818:Animals; D004624:Embryo Transfer / Q000379:methods*; D004622:Embryo, Mammalian / Q000196:embryology*; D047108:Embryonic Development; D005260:Female; D007267:Injections; D008026:Ligation / Q000379:methods; D008297:Male; D051379:Mice; D011247:Pregnancy; D011256:Pregnancy Outcome; D011897:Random Allocation; D012965:Sodium Chloride / Q000008:administration & dosage; D014599:Uterus / Q000601:surgery*
D012965:Sodium Chloride