A well-defined diblock copolymer of poly-(ethylene oxide)-block-poly (4-vinylpyridine) for separation of basic proteins by capillary zone electrophoresis.
A series of well-defined diblock copolymers, poly(ethylene oxide)-block-poly(4-vinylpyridine) (PEO-b-P4VP) used as physical coating of capillaries, were synthesized by atom transfer radical polymerization (ATRP). EOF measurement results showed that all synthesized PEO-b-P4VP diblock copolymer-coated capillaries in this report could suppress EOF effectively compared to the bare fused-silica capillaries, and efficient separations of basic proteins were achieved. The effects of the molecular weight of P4VP block in PEO-b-P4VP and buffer pH on the separation of basic proteins for CE were investigated in detail. Moreover, the relationships between morphologies of PEO-b-P4VP diblock copolymers in buffer, which were studied by transmission electron microscopy, and the separation efficiencies of basic protein with PEO-b-P4VP diblock copolymers coatings were discussed.
Department of Polymer Science and Engineering, Joint Laboratory of Polymer Thin Films and Solution, University of Science and Technology of China, Hefei, P. R. China.
Hang
H
Liu
Ronghua
R
Shi
Wenming
W
Wan
Runmiao
R
Yang
Yanmei
Y
Wang
D001798:Blood Proteins / Q000302:isolation & purification; D053841:Electroosmosis; D019075:Electrophoresis, Capillary / Q000295:instrumentation*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D046529:Microscopy, Electron, Transmission; D011092:Polyethylene Glycols / Q000138:chemical synthesis / Q000737:chemistry*; D015203:Reproducibility of Results; D014753:Vinyl Compounds / Q000138:chemical synthesis / Q000737:chemistry*
D001798:Blood Proteins; D014753:Vinyl Compounds; C520176:poly(4-vinylpyridine)-block-poly(ethylene oxide); D011092:Polyethylene Glycols