Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines.
New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.
Department of Crystallography, Birkbeck College, University of London, London WC1E 7HX, UK.
A J
AJ
Miles
Robert W
RW
Janes
A
A
Brown
D T
DT
Clarke
J C
JC
Sutherland
Y
Y
Tao
B A
BA
Wallace
S V
SV
Hoffmann
D002942:Circular Dichroism; D006801:Humans; D011489:Protein Denaturation / Q000528:radiation effects*; D012709:Serum Albumin / Q000737:chemistry / Q000528:radiation effects; D017356:Synchrotrons
D012709:Serum Albumin