Interactions of KLVFF-PEG peptide conjugate with fibrinogen in neutral aqueous solutions.
In this work we report on the interaction of KLVFF-PEG with fibrinogen (Fbg) in neutral aqueous solutions at 20 degrees C, for particular ratios of KLVFF-PEG to Fbg concentration, Delta = c(KLVFF-PEG)/c(Fbg). Our results show the formation of Fbg/KLVFF-PEG complexes for Delta > 0, such that there is not an extended network of complexes throughout the solution. In addition, cleaved protein and Fbg dimers are identified in the solution for Delta >or= 0. There is a dramatic change in the tertiary structure of the Fbg upon KLVFF-PEG binding, although the KLVFF-PEG binds to the Fbg without affecting the secondary structure elements of the glycoprotein.
School of Chemistry, Food Biosciences and Pharmacy, The University of Reading, Whiteknights, Reading, UK. v.castelletto@reading.ac.uk
Valeria
V
Castelletto
Gemma E
GE
Newby
Ian W
IW
Hamley
D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005340:Fibrinogen / Q000737:chemistry*; D009842:Oligopeptides / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012996:Solutions; D013057:Spectrum Analysis / Q000379:methods; D014867:Water
D009842:Oligopeptides; D012996:Solutions; D014867:Water; D011092:Polyethylene Glycols; D005340:Fibrinogen