Influence of particle size on bacterial community structure in aquatic sediments as revealed by 16S rRNA gene sequence analysis.
Bacterial communities associated with sediment particles were examined using PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing. Particle size influenced community structure, with attached bacterial assemblages separating into 63- to 125-, 125- to 1,000-, and 1,000- to 2,000-microm fractions. Differences were particularly pronounced for the Verrucomicrobia-Planctomycetes, whose numbers were significantly reduced on coarser particles.
Jackson
Colin R
CR
Department of Biology, Shoemaker Hall, The University of Mississippi, University, MS 38677, USA. cjackson@olemiss.edu
Weeks
Andrew Q
AQ
D001419:Bacteria / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D015723:Gene Library; D020459:Genes, rRNA / Q000235:genetics*; D019015:Geologic Sediments / Q000382:microbiology*; D008922:Mississippi; D008969:Molecular Sequence Data; D010316:Particle Size; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D045483:Rivers / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S