Astrocytes facilitate the growth and differentiation of co-cultured mesenchymal stem cells.
The effect of astrocytes on the growth and differentiation of co-cultured mesenchymal stem cells (MSCs) was studied. MSCs of Wistar rats were isolated, cultured and labeled with Hoechst33342. The labeled MSCs were co-cultured with astrocytes in DMEM/F12/10%FBS. The growth status and morphologic change of MSCs were observed. The expression of specific protein NeuN, GFAP and CNP was detected by using immunofluorescence staining. The results showed that the two kinds of co-cultured cells grew well. Some labeled MSCs stretched out long processes with spin, triangle and star shapes. Immunofluorescence stain showed that these cells expressed NeuN, GFAP or CNP. It was suggested that astrocytes could promote the proliferation of co-cultured MSCs and induce them to differentiate into neural like cells.
Chu
Qian
Q
Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. qianchu@tjh.tjmu.edu.cn
Yu
Zhiyuan
Z
Zhang
Suming
S
Yu
Shiying
S
D000818:Animals; D001253:Astrocytes / Q000166:cytology*; D002454:Cell Differentiation; D049109:Cell Proliferation; D017690:Cell Transplantation; D018920:Coculture Techniques / Q000379:methods*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008856:Microscopy, Fluorescence / Q000379:methods; D009457:Neuroglia / Q000166:cytology; D009474:Neurons / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013234:Stem Cells / Q000166:cytology
D005904:Glial Fibrillary Acidic Protein