[Construction and analysis of cDNA library of Yunnan Yuanjiang O. rifupogon leaf].
The cDNA library of Yuanjiang Oryza rifupongon leaf was constructed by using SMART technology. The titers of the non-amplified library and the amplified library were 1.1 x 106 pfu/mL and 3.98 x 107 pfu/mL, respectively. The recombination rate was more than 91%. The DNA sequence length of the most cDNAs in the library was between 500-2 000 bp. Some cDNAs chosen by random were sequenced. After BLAST analysis of some cDNAs, their possible function were predicted. It is found that these cDNAs show 98% similarity to Oryza sativa japonica in the NCBI database. These provided a base for further study on the structure and function of these cDNAs and evolutionary process of Yuanjiang Oryza rifupongon.
School of Life Science, Yunnan University, Kunming 650091, China. sdy88.com@eyou.com
Dong-Yan
DY
Shi
Ming-Zhi
MZ
Yang
Xing-Qi
XQ
Huang
Shan-Na
SN
Chen
Zai-Quan
ZQ
Cheng
D002681:China; D030541:Databases, Genetic; D015723:Gene Library; D012275:Oryza / Q000235:genetics*; D018515:Plant Leaves / Q000235:genetics*; D016133:Polymerase Chain Reaction