Kinetics of multilayer immobilized enzyme-filter reactors: Behavior of urease-filter reactors in different buffers.
A sequential model which describes the kinetic behavior of multilayer immobilized-enzyme filter reactors for systems obeying the Michaelis-Menten scheme and various types of inhibition by product and by substrate is proposed. The model was varified experimentaly using a single enzyme-filter-substrate system-urease bound to nylon-filter disks acting on urea-in different buffers to obtain the various inhibition patterns.
Department of Biochemistry, The George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel.
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Goldstein
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Levy
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Shemer