Carbohydrate arrays as tools for research and diagnostics.
In a very short time, carbohydrate microarrays have become important tools to investigate binding events that involve sugars. High throughput analysis of carbohydrate interactions with a wide range of binding partners, including proteins, RNA, whole cells and viruses, can be performed. Questions ranging from simple binding events to in-depth kinetic analysis can be addressed. This tutorial review summarizes methods to produce carbohydrate microarrays as well as their use. Some selected examples illustrate applications and the potential that these tools hold.
Laboratory for Organic Chemistry, Swiss Federal Institute of Technology (ETH) Zürich, Wolfgang-Pauli-Str. 10, HCI F315, 8093, Zürich, Switzerland.
Tim
T
Horlacher
Peter H
PH
Seeberger
D001665:Binding Sites; D015415:Biomarkers / Q000032:analysis*; D002241:Carbohydrates / Q000032:analysis* / Q000737:chemistry; D002477:Cells / Q000737:chemistry / Q000166:cytology / Q000378:metabolism; D007700:Kinetics; D046228:Microarray Analysis / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012313:RNA / Q000737:chemistry / Q000378:metabolism; D012270:Ribosomes / Q000737:chemistry / Q000378:metabolism; D014780:Viruses / Q000737:chemistry / Q000378:metabolism / Q000472:pathogenicity
D015415:Biomarkers; D002241:Carbohydrates; D011506:Proteins; D012313:RNA