Flow cytometry as a rapid test for detection of penicillin resistance directly in bacterial cells in Enterococcus faecalis and Staphylococcus aureus.
We studied the usefulness of flow cytometry for detection of penicillin resistance in E. faecalis and S. aureus by direct binding of commercially available fluorescent penicillin, Bocillin FL, to cells obtained from culture. There were significantly lower percentages of fluorescent cells and median and mean fluorescence values per particle in penicillin-resistant than in penicillin-sensitive strains of both species observed. The method allows rapid detection of penicillin resistance in S. aureus and E. faecalis. The results encourage further investigations on the detection of antibiotic resistance in bacteria using flow cytometry.
Jarzembowski
T
T
Department of Medical Microbiology, Medical University of Gdańsk, Str. Do Studzienki 38, 80-227 Gdańsk, Poland. tjarzembowski@amg.gda.pl
Wiśniewska
K
K
Józwik
A
A
Bryl
E
E
Witkowski
J
J
D001896:Boron Compounds / Q000378:metabolism; D013293:Enterococcus faecalis / Q000187:drug effects*; D005434:Flow Cytometry / Q000379:methods*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology; D008826:Microbial Sensitivity Tests / Q000379:methods*; D010403:Penicillin Resistance; D010406:Penicillins / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects*
C118961:BOCILLIN FL; D001896:Boron Compounds; D010406:Penicillins