[Oxidative stress is exacerbated in diabetic patients during cardiopulmonary bypass].
Circulation on blood extracorporeally through plastic tubing activates several pathways including systemic inflammation and oxidative stress. These phenomena are suspected to participate to neurological and cardiovascular side effects observed in the patients under cardiopulmonary bypass (CPB). A direct relationship, in diabetic patients, between hyperglycemia and morbidity and mortality has been established. However, it is still unclear whether perioperative hyperglycemia has a direct effect on adverse events in cardiac surgery. The purpose of this study was to determine the influence of hyperglycemia on inflammation and oxidative stress in patients under CPB during cardiac surgery.


METHODS
Control patients (n=17) and diabetic (type 2) patients (n=13) were included in this study. Blood samples were drawn before, during and after the CPB. Oxidative stress was evaluated in the plasma by direct and indirect approaches. Direct detection of ascorbyl radicals was assessed by electron spin resonance spectroscopy. An index: ascorbyl radical/vitamin C ratio is an indicator of the degree of oxidative stress taking place in the plasma. Oxygen radical absorbing capacity (ORAC) values were used as measurement of antioxidant capacity of the plasma. To determine inflammation profile of patients, we measure the evolution of plasma concentration of interleukin 8 (IL-8).


RESULTS
During cross clamping and post-CPB, the index ascorbyl radical/vitamin C is increased; the value of the index is more significant in diabetic patients. Concomitantly, ORAC values decreased in all the patients during cross clamping (p<0.05). Results concerning inflammatory index showed that IL-8 levels increased during the CPB.


CONCLUSIONS
In conclusion, the current study indicates that a systemic oxidative stress occurs during CPB and post-CPB periods and that in patients with type 2 diabetes mellitus, the systemic oxidative stress was increased.
Service d'anesthésie-réanimation, CHU Bocage, Dijon, France.
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Marty
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Bendhadra
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Amoureux
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Guilland
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Vergely
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Rochette
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Girard
D000368:Aged; D001205:Ascorbic Acid / Q000097:blood; D002315:Cardiopulmonary Bypass / Q000009:adverse effects*; D003683:Dehydroascorbic Acid / Q000031:analogs & derivatives / Q000097:blood; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000150:complications* / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis; D019918:Heart Valve Prosthesis Implantation; D006801:Humans; D006943:Hyperglycemia / Q000150:complications; D016209:Interleukin-8 / Q000097:blood; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress
D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid; D006442:Glycated Hemoglobin; D016209:Interleukin-8; C000820:semidehydroascorbic acid