The use of Histochoice for histological examination of articular and growth plate cartilages, intervertebral disc and meniscus.
Histochoice is a proprietary nontoxic, non-cross-linking fixative designed by the manufacturer to replace formaldehyde based fixation protocols. We compared Histochoice and formalin fixation for several cartilaginous tissues including, articular and growth plate cartilage, meniscus and intervertebral disc. The tissues were stained with general histology stains including toluidine blue for tissue proteoglycans, picrosirius red to evaluate collagenous organization, and hematoxylin and eosin to assess cell morphology. The chondroitin sulfate and heparin sulfate substituted proteoglycans aggrecan and perlecan were also immunolocalized in some of the tissues to provide a comparison. Histochoice did not fix deep into the tissue blocks resulting in focal loss of aggrecan and other matrix components from the more central regions of the blocks. This was evident in toluidine blue stained sections of immature tibial articular cartilage where loss of glycosaminoglycan was significant in Histochoice fixed tissues. Histochoice fixation worked well, however, in the aggrecan and perlecan immunohistology applications where its non-cross-linking traits were conducive to epitope retrieval and identification by primary antibodies to extracellular matrix components.
Raymond Purves Research Laboratory, Institute of Bone & Joint Research, Kolling Institute of Medical Research, University of Sydney, Leonards, NSW, Australia. jmelrose@med.usyd.edu.au
J
J
Melrose
S M
SM
Smith
M M
MM
Smith
C B
CB
Little
D000818:Animals; D002358:Cartilage, Articular / Q000166:cytology / Q000378:metabolism*; D005404:Fixatives; D006132:Growth Plate / Q000166:cytology / Q000378:metabolism; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000166:cytology / Q000378:metabolism*; D008297:Male; D008592:Menisci, Tibial / Q000166:cytology / Q000378:metabolism*; D012756:Sheep
D005404:Fixatives