Use of chemically modified activated carbon as a support for immobilized enzymes.
Loading and activity assays of the enzymes alpha-chymotrypsin, alpha-chymotrypsinogen, and glucose oxidase covalently bound to an activated carbon support are presented. The activated carbon support material was pretreated using either a radio-frequency oxygen plasma or an electrochemical oxidation to maximize the enzyme attachment. Cyanuric chloride or water-soluble carbodiimide linking reactions were used to covalently attach the enzymes to the carbon support. Discussion of the relative merits of each reaction scheme is presented.
Osborn
J A
JA
Department of Chemistry, Rutgers University, New Brunswick, New Jersey 08903.
Ianniello
R M
RM
Wieck
H J
HJ
Decker
T F
TF
Gordan
S L
SL
Yacynych
A M
AM