Enhancement of neutralizing humoral response of DNA vaccine against duck hepatitis B virus envelope protein by co-delivery of cytokine genes.
We explored in the duck hepatitis B virus (DHBV) model the impact of duck interferon gamma (Du-IFNgamma) or interleukin 2 (Du-IL2) co-delivery on humoral neutralizing response induced by DNA-based vaccine encoding DHBV preS/S large envelope protein. Co-delivery of either Du-IL2 or Du-IFNgamma encoding plasmids considerably increased the magnitude of anti-preS humoral response. Moreover, co-administration of cytokine genes led to a significant (p<0.001) enhancement of neutralizing anti-DHBV antibody response, which was more pronounced for Du-IFNgamma. Our data suggest that co-delivery of cytokine and envelope protein encoding plasmids will be a valuable approach for the development of a potent therapeutic DNA vaccine against chronic hepatitis B.
INSERM, Unit 871, 151 Cours Albert Thomas, Université Lyon 1, 69008 Lyon, France.
Fadi
F
Saade
Thierry
T
Buronfosse
Pierre
P
Pradat
Fabien
F
Abdul
Lucyna
L
Cova
D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D004372:Ducks; D018347:Hepadnaviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D006510:Hepatitis B Antibodies / Q000097:blood*; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D015611:Hepatitis B Virus, Duck / Q000235:genetics / Q000276:immunology*; D006524:Hepatitis, Viral, Animal / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D007114:Immunization; D007371:Interferon-gamma / Q000235:genetics / Q000276:immunology; D007376:Interleukin-2 / Q000235:genetics / Q000276:immunology; D009500:Neutralization Tests; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*
D016207:Cytokines; D006510:Hepatitis B Antibodies; D017325:Hepatitis B Vaccines; D007376:Interleukin-2; D019444:Vaccines, DNA; D014759:Viral Envelope Proteins; D007371:Interferon-gamma