Synthetic seed production from somatic embryos of Pinus radiata.
Pinus radiata is one of the most important forestry species in the southern hemisphere. This work describes the regeneration of this plant via somatic embryogenesis from immature zygotic embryos. To improve this process, somatic embryogenic cell suspensions were established in liquid media for the generation of material for embryo maturation. Each developmental stage of these suspensions was characterized by microscopy and their growth phases quantified. An alginate-containing medium was used as an encapsulation method for the somatic embryos that were then germinated as artificial seeds in vitro. The protocols described in this work are both useful and reliable for industrial purposes.
Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, PO Box 114-D, Santiago, Chile.
Felipe
F
Aquea
María Josefina
MJ
Poupin
José Tomás
JT
Matus
Marlene
M
Gebauer
Consuelo
C
Medina
Patricio
P
Arce-Johnson
D003470:Culture Media; D018525:Germination; D028223:Pinus / Q000196:embryology* / Q000254:growth & development; D012639:Seeds / Q000166:cytology
D003470:Culture Media