Antiacetylcholinesterase and antioxidant ent-Kaurene diterpenoid, melissoidesin from Isodon wightii (Bentham) H. Hara.
The ent kaurene diterpenoid, melissoidesin was isolated from the acetone extract of the leaves of Isodon wightii and the structure was designated as 3beta, 11beta, 15beta-trihydroxy-6alpha-acetoxy-ent-kaur-16-ene based on spectral data and previous reports. Melissoidesin isolated from the acetone extract of leaves showed potent antiacetylcholinesterase activity and the IC(50) value was observed as 215 microg mL(-1). DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radical scavenging activity of melissoidesin was significant and the IC(50) value was 138 microg mL(-1). The significant reducing property of the melissoidesin was stronger in high concentration. IC(50) value of melissoidesin on hydroxyl radicals and metal chelation was observed as 99 and 143 microg mL(-1), respectively. The 50% inhibitory concentration of melissoidesin on lipid peroxidation was calculated as 133 microg mL(-1). These findings indicate that ent kaurene diterpenoid, melissoidesin was promising antiacetylcholinesterase and antioxidant which can be used as food and drug preparations.
Thirugnanasampandan
R
R
Department of Botany, Bharathiar University, Coimbatore-641046, Tamil Nadu, India. thirugnanasampandan@yahoo.co.in
Jayakumar
R
R
Narmatha Bai
V
V
Martin
Ebenezar
E
Rajendra Prasad
K J
KJ
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000302:isolation & purification*; D045786:Diterpenes, Kaurane / Q000737:chemistry / Q000302:isolation & purification*; D037602:Isodon / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry
D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D045786:Diterpenes, Kaurane; D010936:Plant Extracts; C530841:melissoidesin