Transient hyperaemic response to assess vascular reactivity of skin: effect of topical anaesthesia.
BACKGROUND
Local anaesthetics affect blood vessels in various ways, depending upon the site of action and the drug used. There is controversy over the vascular effects of two widely used topical local anaesthetic agents, EMLA and Ametop. We used the transient hyperaemic response (THR) to the brief compression of the brachial artery to assess vascular reactivity of forearm skin after application of topical local anaesthesia.


METHODS
Twenty healthy male volunteers were studied. Forearm blood flow-flux was measured using laser Doppler flowmetry, and the magnitude of the hyperaemic response to brachial artery occlusion for 20 s was recorded. Control cream, EMLA, or Ametop were applied and covered with an occlusive dressing for 60 min. Blood flow-flux and the hyperaemic response measurements were then repeated, with the laser Doppler probes sited over the areas of skin to which the local anaesthetic creams had been applied. Measurements were made at 60, 90, and 120 min after the application of the creams. The THR ratio (THRR) was calculated at each time point, defined as the net hyperaemic flow-flux divided by the baseline flow-flux.


RESULTS
At 60 min, Ametop caused a significantly greater increase in blood flow and decrease in THRR over control than EMLA [net increase of mean blood flow (SD) over control 95 (61) vs 2 (17) AU (P<0.001), net mean THRR decrease over control 1.33 (1.85) vs -0.34 (1.33) (P<0.02)].


CONCLUSIONS
The application of topical Ametop decreases microvascular tone and vasoreactivity of the forearm skin in healthy volunteers.
University Department of Anaesthesia, Nottingham University Hospitals NHS Trust, Queen's Medical Centre Campus, Nottingham NG7 2UH, UK.
M D
MD
Wiles
E
E
Dickson
I K
IK
Moppett
D000328:Adult; D000779:Anesthetics, Local / Q000494:pharmacology*; D001783:Blood Flow Velocity / Q000187:drug effects; D005542:Forearm / Q000098:blood supply; D006801:Humans; D006940:Hyperemia / Q000139:chemically induced* / Q000503:physiopathology; D017078:Laser-Doppler Flowmetry; D008012:Lidocaine / Q000494:pharmacology; D000077442:Lidocaine, Prilocaine Drug Combination; D008297:Male; D008833:Microcirculation / Q000187:drug effects; D011318:Prilocaine / Q000494:pharmacology; D012039:Regional Blood Flow / Q000187:drug effects; D012867:Skin / Q000098:blood supply*; D013748:Tetracaine / Q000494:pharmacology; D014664:Vasodilation / Q000187:drug effects
D000779:Anesthetics, Local; D000077442:Lidocaine, Prilocaine Drug Combination; D011318:Prilocaine; D013748:Tetracaine; D008012:Lidocaine