Local Th1/Th2 cytokine expression in experimental murine vaginal candidiasis.
In order to investigate the expression of Th1 and Th2 cytokines in the vaginal candidiasis caused by Candida, the fungal vaginitis model was established in female ICR mice by intravaginal inoculation of suspension of C. albicans after the animals were pretreated with estradiol. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of IL-2, IL-4, IL-10 and TGF-beta1 in the vagina in the mice of different groups at different time points after the beginning of the experiment. The average expression level of IL-2 mRNA in group D (estrogen-treated mice) was significantly higher than that in groups H (estrogen-untreated mice) and I (control group) on the day 2. The average expression level of IL-4 mRNA in group D was significantly higher than that in groups I and H on the day 5. The average expression level of IL-10 mRNA in group D was significantly higher than that in groups H and I from day 7 to 11. The average expression level of TGF-beta1 mRNA in group D was significantly higher than that in groups H and I at all time points. It was concludes that the high-level expression of IL-2 mRNA during early infection was associated with clearance of mucosal C. albicans, and the high-level expression of IL-10 mRNA during late stage of the infection was related to susceptibility to infection. TGF-beta1 may play a predominant role when the virtual absence of changes in other Th-type cytokines during infection.
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. oY6222@yahoo.com.cn
Weixiang
W
Ouyang
Shanjuan
S
Chen
Zhixiang
Z
Liu
Yan
Y
Wu
Jiawen
J
Li
D000818:Animals; D002176:Candida albicans / Q000378:metabolism; D002181:Candidiasis, Vulvovaginal / Q000175:diagnosis* / Q000382:microbiology*; D016207:Cytokines / Q000096:biosynthesis*; D005260:Female; D005786:Gene Expression Regulation; D016753:Interleukin-10 / Q000096:biosynthesis; D007376:Interleukin-2 / Q000096:biosynthesis; D015847:Interleukin-4 / Q000096:biosynthesis; D051379:Mice; D008813:Mice, Inbred ICR; D018417:Th1 Cells / Q000378:metabolism*; D018418:Th2 Cells / Q000378:metabolism*; D053773:Transforming Growth Factor beta1 / Q000096:biosynthesis; D014621:Vagina / Q000382:microbiology* / Q000473:pathology
D016207:Cytokines; D007376:Interleukin-2; D053773:Transforming Growth Factor beta1; D016753:Interleukin-10; D015847:Interleukin-4