Local tissue destruction and procoagulation properties of Echis carinatus venom: inhibition by Vitis vinifera seed methanol extract.
Plant extracts are extensively used against snakebites in Indian folk medicine. In this study, one such traditionally used plant, Vitis vinifera L. (Vitaceae) seed methanol extract has been studied for its ability to neutralize Indian Echis carinatus (saw-scaled viper) venom. The extract effectively inhibited toxic effects, such as oedema, haemorrhage, myonecrosis and coagulation of citrated human plasma. Further, the extract inhibited the caseinolytic, hyaluronolytic and fibrinogenolytic activities of the venom. The extract caused dose dependent inhibition of the toxic activities studied, suggesting venom inhibition. Thus, the anti-snake venom property of the extract appears to be highly promising for further investigation in order to achieve better neutralization of Indian E. carinatus venom poisoning.
Department of Studies in Biochemistry, University of Mysore, Mysore 570006, India.
Y H
YH
Mahadeswaraswamy
S
S
Nagaraju
K S
KS
Girish
K
K
Kemparaju
D000818:Animals; D000997:Antivenins / Q000494:pharmacology*; D001777:Blood Coagulation / Q000187:drug effects; D002364:Caseins / Q000378:metabolism; D003402:Creatine Kinase / Q000378:metabolism; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D006470:Hemorrhage / Q000139:chemically induced / Q000188:drug therapy; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D051379:Mice; D018482:Muscle, Skeletal / Q000187:drug effects / Q000201:enzymology / Q000473:pathology; D009336:Necrosis / Q000139:chemically induced / Q000473:pathology; D009500:Neutralization Tests; D008517:Phytotherapy; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D014757:Viper Venoms / Q000037:antagonists & inhibitors / Q000633:toxicity*; D027843:Vitis / Q000737:chemistry*
D000997:Antivenins; D002364:Caseins; D007155:Immunologic Factors; D010936:Plant Extracts; D014757:Viper Venoms; D007770:L-Lactate Dehydrogenase; D003402:Creatine Kinase