Detection of a novel intergenogroup recombinant Norovirus from Kolkata, India.
Mutation and recombination are recognized as important driving forces of evolution among RNA viruses. An intergenogroup recombinant norovirus strain [Hu/Kol/NLV/L8775/AB290150/2006/India] was detected in the faecal specimen of a 17 year old male, who had suffered from acute watery diarrhea and severe dehydration. Sequence analysis confirmed that this novel recombinant strain had a polymerase gene fragment that closely resembled a Norovirus (NoV) genogroup-I genotype-3 virus (HuCV/NLV/GI.3/VA98115/AY038598/1998/USA) and a capsid gene resembling NoV genogroup-II genotype-4 virus (NoV/Hu/GII.4/Terneuzen70/EF126964/2006/NL). The crossing over and recombination was observed at nucleotide (nt) 790 of NoV GI VA98115 strain and nt808 of NoV GII Terneuzen70 strain. In both parent strains conserved nucleotide sequence and hairpin structure (DNA secondary structure) were reported at the junction point of ORF1 and ORF2, exhibiting the mechanism of recombination in these viruses. Thus this novel recombinant NoV is another step in evolution among NoVs, indicating that constant surveillance is important to successfully monitor emergence of these strains.
Nayak
Mukti K
MK
Molecular Virology Laboratory, Diarrhoeal Disease Research and Control Centre, Division of Virology, National Institute of Cholera and Enteric Diseases, Beliaghata, Kolkata, 700010, India.
Balasubramanian
Ganesh
G
Sahoo
Ganesh C
GC
Bhattacharya
Rittwika
R
Vinje
Jan
J
Kobayashi
Nobumichi
N
Sarkar
Mamta Chawla
MC
Bhattacharya
Mihir K
MK
Krishnan
Triveni
T
D000293:Adolescent; D001483:Base Sequence; D017250:Caliciviridae Infections / Q000821:virology; D036022:Capsid Proteins / Q000235:genetics; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005759:Gastroenteritis / Q000821:virology; D006801:Humans; D007194:India; D008297:Male; D008969:Molecular Sequence Data; D029322:Norovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D009690:Nucleic Acid Conformation; D016366:Open Reading Frames; D010802:Phylogeny; D011995:Recombination, Genetic; D012689:Sequence Homology, Nucleic Acid
D036022:Capsid Proteins; D017931:DNA Primers; D004279:DNA, Viral