Quantitative trait loci for glucosinolate accumulation in Brassica rapa leaves.
Glucosinolates and their breakdown products have been recognized for their effects on plant defense, human health, flavor and taste of cruciferous vegetables. Despite this importance, little is known about the regulation of the biosynthesis and degradation in Brassica rapa. Here, the identification of quantitative trait loci (QTL) for glucosinolate accumulation in B. rapa leaves in two novel segregating double haploid (DH) populations is reported: DH38, derived from a cross between yellow sarson R500 and pak choi variety HK Naibaicai; and DH30, from a cross between yellow sarson R500 and Kairyou Hakata, a Japanese vegetable turnip variety. An integrated map of 1068 cM with 10 linkage groups, assigned to the international agreed nomenclature, is developed based on the two individual DH maps with the common parent using amplified fragment length polymorphism (AFLP) and single sequence repeat (SSR) markers. Eight different glucosinolate compounds were detected in parents and F(1)s of the DH populations and found to segregate quantitatively in the DH populations. QTL analysis identified 16 loci controlling aliphatic glucosinolate accumulation, three loci controlling total indolic glucosinolate concentration and three loci regulating aromatic glucosinolate concentrations. Both comparative genomic analyses based on Arabidopsis-Brassica rapa synteny and mapping of candidate orthologous genes in B. rapa allowed the selection of genes involved in the glucosinolate biosynthesis pathway that may account for the identified QTL.
Laboratory of Plant Breeding, Wageningen University, 6700AJ, Wageningen, the Netherlands.
Ping
P
Lou
Laboratory of Plant Breeding, Wageningen University, 6700AJ, Wageningen, the Netherlands.|Horticultural College, Hebei Agricultural University, 071001, Baoding, China.|Laboratory of Genetics, Wageningen University, 6703BD, Wageningen, the Netherlands.
Jianjun
J
Zhao
Product Design and Quality Management Group, Wageningen University, 6700EV, Wageningen, the Netherlands.|National Engineering Research Center for Vegetables, 100081, Beijing, China.
Hongju
H
He
Laboratory of Genetics, Wageningen University, 6703BD, Wageningen, the Netherlands.
Corrie
C
Hanhart
Laboratory of Plant Breeding, Wageningen University, 6700AJ, Wageningen, the Netherlands.
Dunia
D
Pino Del Carpio
Product Design and Quality Management Group, Wageningen University, 6700EV, Wageningen, the Netherlands.
Ruud
R
Verkerk
Plant Research International, Wageningen University and Research Centre, 6708PB, Wageningen, the Netherlands.
Jan
J
Custers
Laboratory of Genetics, Wageningen University, 6703BD, Wageningen, the Netherlands.|Max Planck Institute for Plant Breeding Research, 50829, Cologne, Germany.
Maarten
M
Koornneef
Laboratory of Plant Breeding, Wageningen University, 6700AJ, Wageningen, the Netherlands.
Guusje
G
Bonnema
D029741:Brassica rapa / Q000235:genetics* / Q000378:metabolism; D002874:Chromosome Mapping; D008040:Genetic Linkage; D005961:Glucosinolates / Q000378:metabolism*; D006238:Haploidy; D018515:Plant Leaves / Q000235:genetics / Q000378:metabolism; D040641:Quantitative Trait Loci
D005961:Glucosinolates