Evaluation of Newcastle disease virus immunoassays for waterfowl using a monoclonal antibody specific for the duck immunoglobulin light chain.
In the present study a monoclonal antibody (mAb 14A3) was tested for its reactivity against serum immunoglobulin Y (IgY) of several waterfowl species, and subsequently for its applicability as anti-species antibody in common immunoassays. Western blot analyses demonstrated its broad cross-reactivity with the serum IgY light chain of different duck species: Muscovy duck (Cairina moschata), Mallard (Anas platyrhynchos), white-winged wood duck (Asarcornis scutulatus), common pintail (Dafila acuta). Reactivity was also evident with IgY of two swan species--mute swan (Cygnus olor) and black-necked swan (Sthenelides melanocoryphus)--and two goose species--domestic goose (Anser anser var. domestica) and red-breasted goose (Rufibrenta ruficollis). Applying the mAb for Newcastle disease virus (avian paramyxovirus serotype 1 [APMV-1]) test systems, its functionality within indirect immunoassays was evaluated. Using APMV-1-positive sera of domestic geese and Muscovy ducks, mAb 14A3 facilitated specific staining of APMV-1-infected cells in an immunofluorescence test. In addition, it proved to be functional in an indirect enzyme-linked immunosorbent assay (ELISA) and a western blot assay. Thus, the analysed mAb represents an attractive and versatile reagent that offers the opportunity to develop serological tests for waterfowl, allowing a high sample throughput using the ELISA technique or the fine analysis of humoral immune responses using the western blot.
Institute for Animal Physiology, University of Munich, Munich, Germany.
Sonja
S
Kothlow
Rafaela
R
Haüslaigner
Bernd
B
Kaspers
Christian
C
Grund
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D004372:Ducks / Q000235:genetics; D007118:Immunoassay / Q000662:veterinary*; D007147:Immunoglobulin Light Chains / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology; D009521:Newcastle Disease / Q000175:diagnosis / Q000821:virology; D009522:Newcastle disease virus / Q000276:immunology*; D010802:Phylogeny; D013997:Time Factors; D014765:Viral Vaccines / Q000276:immunology
D000911:Antibodies, Monoclonal; C016512:IgY; D007147:Immunoglobulin Light Chains; D007136:Immunoglobulins; D014765:Viral Vaccines