Detection of C-reactive protein on a functional poly(thiophene) self-assembled monolayer using surface plasmon resonance.
The preparation of a new poly(thiophene) with pendant N-hydroxysuccinimide ester groups and its application to immobilization of biomolecules are reported. A thiophene derivative of N-hydroxysuccinimide ester was polymerized with FeCl3 in chloroform and the resulting poly(thiophene) was characterized by nuclear magnetic resonance (NMR), Fourier transform infrared (FT-IR), and gel permeation chromatography (GPC). This polymer reacts with amine-bearing molecules to yield new poly(thiophene) derivatives and the specific interactions at the side groups could be detected. Thus, a self-assembled monolayer (SAM) using the polymer was formed on a gold-coated quartz cell and anti-C-reactive protein (anti-CRP) was immobilized. The binding behavior of CRP on the surface was monitored by use of a surface plasmon resonance (SPR) sensor system.
Kim
Hyun-Chul
HC
Division of Nano-Bio Technology, Daegu Gyeongbuk Institute of Science and Technology, Daegu 704-230, Republic of Korea.
Lee
Soo-Keun
SK
Jeon
Won Bae
WB
Lyu
Hong-Kun
HK
Lee
Seung Woo
SW
Jeong
Sang Won
SW
D015374:Biosensing Techniques; D002097:C-Reactive Protein / Q000032:analysis* / Q000737:chemistry; D006046:Gold / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D020349:Surface Plasmon Resonance / Q000379:methods; D013876:Thiophenes / Q000737:chemistry
D011108:Polymers; D013876:Thiophenes; D006046:Gold; D002097:C-Reactive Protein