Layer-by-layer assembly of a conformal nanothin PEG coating for intraportal islet transplantation.
Encapsulation of cells and tissue offers a rational approach for attenuating deleterious host responses toward transplanted cells, but a need exists to develop cell encapsulation strategies that minimize transplant volume. In this report, we describe the formation of nanothin, PEG-rich conformal coatings on individual pancreatic islets via layer-by-layer self-assembly of poly( l-lysine)- g-poly(ethylene glycol)(biotin) (PPB) and streptavidin (SA). Through control of grafting ratio, PPB could be rendered nontoxic and facilitated growth of PPB/SA multilayer thin films that conformed to the heterogeneous islet surface. (PPB/SA) 8 multilayer films could be assembled without loss of islet viability or function, and coated islets performed comparably to untreated controls in vivo in a murine model of allogenic intraportal islet transplantation.
Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
John T
JT
Wilson
Wanxing
W
Cui
Elliot L
EL
Chaikof
D002467:Cell Nucleus; D006801:Humans; D016381:Islets of Langerhans Transplantation / Q000379:methods; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D055672:Static Electricity; D046509:Tissue Culture Techniques
D011092:Polyethylene Glycols