Pilot-plant production of prednisolone using calcium alginate immobilized Arthrobacter simplex.
The maximum conversion of hydrocortisone suspensions at initial substrate concentrations greater than 4 g/L by immobilized Arthrobacter simplex in a batch reactor was 80-85%. By feeding hydrocortisone suspensions continuously to either a fed-batch-operated stirred tank reactor or to a continuous-flow airlift loop reactor, at a rate such that the soluble hydrocortisone concentration in the reactor remained ca. 0.05 g/L, 95% conversion of substratewas obtained at final product concentrations exceeding 4 g/L.
Department of Chemical and Biochemical Engineering, University College London. Torrington Place, London. WCIE 7JE, England.
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Kloosterman
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Lilly