A corticotropin-releasing hormone binding protein (CRH-BP) gene from the intertidal copepod, Tigriopus japonicus.
The corticotropin-releasing hormone (CRH) plays a critical role in stress-response regulation in vertebrates. The activity of CRH depends on CRH-binding protein (CRH-BP). CRH-BP is considered to play a chaperoning role in stress. Limited information mainly from the insects is available on the molecular structure and functions of invertebrate CRH and CRH-BP. We cloned and sequenced a CRH-BP gene from the intertidal copepod, Tigriopus japonicus which was expressed at all the stages of development. Molecular phylogenetic analysis showed that T. japonicus CRH-BP was closely related to CRH-BP of honeybee and other insects. The highest level of CRH-BP transcripts was expressed in adult males followed by nauplius stage 1. The expression of CRH-BP was upregulated when T. japonicus was subjected to temperature or salinity stress. This study demonstrates that CRH-BP in T. japonicus might play a role in stress-response. However, establishing such a role demands further studies on CRH-BP from other invertebrates and their expression under stress.
Lee
Kyun-Woo
KW
Department of Chemistry, College of Natural Sciences, Hanyang University, 17 Haengdang-dong, Seongdong-gu, Seoul 133-791, South Korea.
Rhee
Jae-Sung
JS
Raisuddin
Sheikh
S
Gi Park
Heum
H
Lee
Jae-Seong
JS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D003001:Cloning, Molecular; D033342:Copepoda / Q000235:genetics* / Q000254:growth & development; D005260:Female; D018507:Gene Expression Regulation, Developmental; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D017386:Sequence Homology, Amino Acid; D013312:Stress, Physiological / Q000235:genetics
D002352:Carrier Proteins; D012333:RNA, Messenger; C067133:corticotropin releasing factor-binding protein