Dynamics of femtosecond laser photo-modification of collagen fibers.
In this work, we investigate the non-ablative, non-thermal photo-modification of collagen fibers by femtosecond Ti:Sa laser. The effect was induced and simultaneously registered during the repetitive laser scanning of type I collagen (rat tail and bovine Achilles' tendon), and bovine cornea. An irreversible increase in two-photon autofluorescence and a decrease in second harmonic generation intensities were associated with the collagen femtosecond laser photo-modification. Confocal spectral imaging revealed the formation of new fluorescent species. Controllable nonlinear photo-modification of collagen fibers and bovine cornea with approximately 2 microm spatial resolution was demonstrated.
Department of Physics, National Taiwan University, Taipei 106, Taiwan.
Vladimir
V
Hovhannisyan
Wen
W
Lo
Chieh
C
Hu
Shean-Jen
SJ
Chen
Chen Yuan
CY
Dong
D024022:Fibrillar Collagens / Q000737:chemistry* / Q000528:radiation effects* / Q000648:ultrastructure; D007834:Lasers; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D011829:Radiation Dosage
D024022:Fibrillar Collagens