Basal and UV-induced MMP-1 expression are inhibited by p53 in human dermal fibroblasts.
Ultraviolet (UV) triggers induction of matrix metalloproteinase-1 (MMP-1) and also induces the rapid appearance of p53 protein in the epidermis and dermal fibroblasts. However, little is known about the role of p53 on MMP-1 expression in human skin. Here, we investigated the effects of p53 on basal and UV-induced MMP-1 in human dermal fibroblasts. To verify the role of p53 on MMP-1 expression, adenoviral p53 (Ad-p53) gene was infected to human dermal fibroblasts. Our results showed that basal and UV-induced MMP-1 expression was prevented by Ad-p53. In contrast, p53 inhibitor, pifithrin-alpha augmented the UV-induced MMP-1 expression. On the other hand, p53 activator, etoposide, decreased the MMP-1 expression in both normal and p53-overexpressed cells. In addition, MMP-1 expression was not changed by etoposide and/or pifithrin-alpha in HaCaT cells, which have mutation of p53. Taken together, we suggest that p53 inhibits basal and UV-induced MMP-1 expression in human dermal fibroblasts.
Department of Dermatology, Seoul National University College of Medicine, Seoul National University Hospital, Seoul National University, Seoul, Korea.
Sangmin
S
Kim
Youngae
Y
Lee
Dong Hun
DH
Lee
Yoonkyung
Y
Kim
Kwang Hyun
KH
Cho
Jin Ho
JH
Chung
D000256:Adenoviridae / Q000235:genetics; D052160:Benzothiazoles / Q000494:pharmacology; D015153:Blotting, Western; D002478:Cells, Cultured; D020405:Dermis / Q000166:cytology / Q000378:metabolism; D005047:Etoposide / Q000494:pharmacology; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects; D006801:Humans; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism*; D010766:Phosphorylation / Q000187:drug effects / Q000528:radiation effects; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D014050:Toluene / Q000031:analogs & derivatives / Q000494:pharmacology; D014161:Transduction, Genetic; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000502:physiology*; D014466:Ultraviolet Rays; D055815:Young Adult
D052160:Benzothiazoles; D016760:Proto-Oncogene Proteins c-fos; D016755:Proto-Oncogene Proteins c-jun; D016159:Tumor Suppressor Protein p53; D014050:Toluene; D005047:Etoposide; C121565:pifithrin; D020781:Matrix Metalloproteinase 1