Pretreatment of indian cane molasses for increased production of citric acid.
Surface culture citric acid fermentation was carried out by Aspergillus niger T55, a strain isolated from its natural source, using cane molasses, either untreated or treated by various methods. Citric acid biosynthesis is seriously impaired by both organic and inorganic inhibitors. A combined treatment of molasses with tricalcium phosphate, hydrochloric acid, and Sephadex fractionation minimizes the level of inorganic and organic inhibitors in molasses and increases the production of citric acid (65% weight yield based on total reducing sugar). The optimum level of individual metal ions for citric acid production depends on the concentration of other metals in the medium.
Biochemical Engineering Research Centre, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi-110016, India.
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Kundu
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Panda
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Majumdar
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Guha
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Bandyopadhyay