Cellular localization of interleukin 13 receptor alpha2 in human primary bronchial epithelial cells and fibroblasts.
BACKGROUND
Interleukin (IL) 13 is a key cytokine in asthma, regulating fibrosis, airway remodeling, induction of immunoglobulin E synthesis by B cells, bronchial hyperresponsiveness, and mucus production. IL-13 signals through the type II IL-4 receptor (IL-4R), which is composed of the IL-4Ralpha and the IL-13Ralpha1 chains. Another IL-13 binding chain, IL-13Ralpha2, binds IL-13 with high affinity but has no known signaling capability and is thought to serve as a decoy receptor providing tight regulation of IL-13 responses.


METHODS
In this study, we investigated the cellular localization of IL-13Ralpha2 in human primary bronchial epithelial cells and fibroblasts using flow cytometry and confocal microscopy, as well as the in vivo expression of IL-13Ralpha2 in the human bronchial mucosa by means of immunohistochemistry.


RESULTS
IL-13Ralpha2 is predominantly an intracellular rather than a membrane-bound molecule in both human primary bronchial epithelial cells and fibroblasts and displays a diffuse granular cytoplasmic distribution in both cell types. IL-13Ralpha2 protein is expressed in vivo in the human bronchial mucosa with its expression being higher in bronchial epithelial cells than bronchial fibroblasts both in vivo and in vitro.


CONCLUSIONS
IL-13Ralpha2 is expressed by both human primary bronchial epithelial cells and fibroblasts as an intracellular protein with a diffuse cytoplasmic distribution. In vivo, IL-13Ralpha2 is expressed in the human airway mucosa mainly by bronchial epithelial cells.
Konstantinidis
A K
AK
Division of Infection, Inflammation and Repair, School of Medicine, University of Southampton, Southampton, UK.
Puddicombe
S M
SM
Mochizuki
A
A
Sheth
P D
PD
Yang
I A
IA
Yoshisue
H
H
Wilson
S J
SJ
Davies
D E
DE
Holgate
S T
ST
Holloway
J W
JW
D000328:Adult; D001980:Bronchi / Q000166:cytology / Q000276:immunology*; D002460:Cell Line; D002478:Cells, Cultured; D003593:Cytoplasm / Q000276:immunology; D004847:Epithelial Cells / Q000166:cytology / Q000276:immunology*; D005347:Fibroblasts / Q000166:cytology / Q000276:immunology*; D006801:Humans; D053663:Interleukin-13 Receptor alpha2 Subunit / Q000378:metabolism*; D020545:Respiratory Mucosa / Q000166:cytology / Q000276:immunology*; D020298:U937 Cells
D053663:Interleukin-13 Receptor alpha2 Subunit