Gene expression response in target organ and whole blood varies as a function of target organ injury phenotype.
This report details the standardized experimental design and the different data streams that were collected (histopathology, clinical chemistry, hematology and gene expression from the target tissue (liver) and a bio-available tissue (blood)) after treatment with eight known hepatotoxicants (at multiple time points and doses with multiple biological replicates). The results of the study demonstrate the classification of histopathological differences, likely reflecting differences in mechanisms of cell-specific toxicity, using either liver tissue or blood transcriptomic data.
Cogenics, Division of Clinical Data, Inc, Morrisville, NC 27560, USA. elobenhofer@cogenics.com
Edward K
EK
Lobenhofer
J Todd
JT
Auman
Pamela E
PE
Blackshear
Gary A
GA
Boorman
Pierre R
PR
Bushel
Michael L
ML
Cunningham
Jennifer M
JM
Fostel
Kevin
K
Gerrish
Alexandra N
AN
Heinloth
Richard D
RD
Irwin
David E
DE
Malarkey
B Alex
BA
Merrick
Stella O
SO
Sieber
Charles J
CJ
Tucker
Sandra M
SM
Ward
Ralph E
RE
Wilson
Patrick
P
Hurban
Raymond W
RW
Tennant
Richard S
RS
Paules
D000818:Animals; D001769:Blood / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D008099:Liver / Q000293:injuries* / Q000378:metabolism*; D008297:Male; D051381:Rats; D043922:Toxicogenetics / Q000379:methods*