A serine protease inhibitor from hemolymph of green mussel, Perna viridis.
Bioactivity guided fractions of cell-free hemolymph of bacterially challenged marine mussel, Perna viridis led to the isolation of a novel quaternary alkaloid 1, which was identified by its spectral data. The isolated molecule 1 has been found to be a potent serine protease inhibitor (SPI) showing IC(50) and K(i) values of 102.5 and 97.1-104.68 microM, respectively. The E(t)/K(i) value of SPI is 6.3, whereas E(t)/K(m) value is 1.04. The Van't Hoff analysis showed that the value of K(i) decreases with increase in temperature, and the binding of the inhibitor is entropically driven.
Division of Biochemical Science, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411 008, India.
M S
MS
Khan
U
U
Goswami
S R
SR
Rojatkar
M I
MI
Khan
D000470:Alkaloids / Q000737:chemistry; D000818:Animals; D001682:Biological Availability; D002474:Cell-Free System; D002626:Chemistry, Pharmaceutical / Q000379:methods; D015195:Drug Design; D006458:Hemolymph / Q000378:metabolism*; D020128:Inhibitory Concentration 50; D007700:Kinetics; D008956:Models, Chemical; D049883:Perna; D015842:Serine Proteinase Inhibitors / Q000138:chemical synthesis* / Q000494:pharmacology; D013696:Temperature; D013816:Thermodynamics; D014357:Trypsin / Q000737:chemistry
D000470:Alkaloids; D015842:Serine Proteinase Inhibitors; D014357:Trypsin