Haemophilus influenzae interacts with the human complement inhibitor factor H.
Pathogenic microbes acquire human complement inhibitors to circumvent the innate immune system. In this study, we identify two novel host-pathogen interactions, factor H (FH) and factor H-like protein 1 (FHL-1), the inhibitors of the alternative pathway that binds to Hib. A collection of clinical Haemophilus influenzae isolates was tested and the majority of encapsulated and unencapsulated bound FH. The isolate Hib 541 with a particularly high FH-binding was selected for detailed analysis. An increased survival in normal human serum was observed with Hib 541 as compared with the low FH-binding Hib 568. Interestingly, two binding domains were identified within FH; one binding site common to both FH and FHL-1 was located in the N-terminal short consensus repeat domains 6-7, whereas the other, specific for FH, was located in the C-terminal short consensus repeat domains 18-20. Importantly, both FH and FHL-1, when bound to the surface of Hib 541, retained cofactor activity as determined by analysis of C3b degradation. Two H. influenzae outer membrane proteins of approximately 32 and 40 kDa were detected with radiolabeled FH in Far Western blot. Taken together, in addition to interactions with the classical, lectin, and terminal pathways, H. influenzae interferes with the alternative complement activation pathway by binding FH and FHL-1, and thereby reducing the complement-mediated bactericidal activity resulting in an increased survival. In contrast to incubation with active complement, H. influenzae had a reduced survival in FH-depleted human serum, thus demonstrating that FH mediates a protective role at the bacterial surface.
Hallström
Teresia
T
Medical Microbiology, Department of Laboratory Medicine, Lund University, Malmö University Hospital, Sweden.
Zipfel
Peter F
PF
Blom
Anna M
AM
Lauer
Nadine
N
Forsgren
Arne
A
Riesbeck
Kristian
K
D003180:Complement C3b Inactivator Proteins; D017242:Complement Factor H / Q000276:immunology / Q000378:metabolism; D003165:Complement System Proteins / Q000276:immunology*; D006193:Haemophilus influenzae / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D007477:Ions / Q000737:chemistry; D011485:Protein Binding; D053586:Virus Internalization; D019096:Vitronectin / Q000378:metabolism
C493495:CFH protein, human; C517621:CFHR1 protein, human; D003180:Complement C3b Inactivator Proteins; D007477:Ions; D019096:Vitronectin; D017242:Complement Factor H; D003165:Complement System Proteins