Three-dimensional imaging in three-dimensional optical multi-beam micromanipulation.
In this work, we present a method providing real-time, low cost, three-dimensional imaging in a three-dimensional optical micromanipulation system. The three-dimensional imaging system is based on a small form factor LED based projector. The projector is used to dynamically shape the rear illumination light in a counter-propagating beam-trapping setup. This allows us to produce stereoscopic images, from which the human brain can construct a three-dimensional image, or alternatively image analysis can be applied by a computer, thereby obtaining true three-dimensional coordinates in real-time for the trapped objects.
Department of Photonics Engineering, DTU Fotonik, Technical University of Denmark, DK-4000 Roskilde, Denmark.
Jeppe S
JS
Dam
Ivan R
IR
Perch-Nielsen
Darwin
D
Palima
Jesper
J
Glückstad
D000465:Algorithms; D001331:Automation; D001921:Brain / Q000473:pathology*; D002138:Calibration; D003196:Computer Graphics; D003952:Diagnostic Imaging / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D021621:Imaging, Three-Dimensional / Q000295:instrumentation / Q000379:methods*; D008027:Light; D008846:Micromanipulation / Q000295:instrumentation / Q000379:methods*; D015233:Models, Statistical