A methoxylated fatty acid isolated from the brown seaweed Ishige okamurae inhibits bacterial phospholipase A2.
A methoxylated fatty acid that inhibits phospholipase A(2) (PLA(2); EC 3.1.1.4) was purified from the brown seaweed Ishige okamurae. Approximately 8.1 mg of the inhibitory compound, 7-methoxy-9-methylhexadeca-4,8-dienoic acid, was isolated from 1 kg of I. okamurae powder. Recombinant PLA(2) derived from the pathogenic bacterium Vibrio mimicus was used as the target enzyme. The methoxylated fatty acid compound competitively inhibited PLA(2) with a Ki value of 3.9 microg/mL. The concentrations required for 50% inhibition of PLA(2), oedema and erythema were 1.0 microg/mL, 3.6 mg/mL and 4.6 mg/mL, respectively. The compound strongly inhibited PLA(2) activity in vitro and had potent antiinflammatory activity in vivo.
Cho
Ji-Young
JY
Department of Marine Biotechnology, Soonchunhyang University, Asan 336-745, Korea.
Gyawali
Yadav P
YP
Ahn
Sun-Hee
SH
Khan
Mohammed N A
MN
Kong
In-Soo
IS
Hong
Yong-Ki
YK
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D004195:Disease Models, Animal; D004431:Ear, External / Q000187:drug effects / Q000473:pathology; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005227:Fatty Acids / Q000737:chemistry / Q000494:pharmacology*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D000459:Phaeophyceae / Q000737:chemistry*; D064801:Phospholipase A2 Inhibitors; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D011994:Recombinant Proteins; D044164:Vibrio mimicus / Q000201:enzymology*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D004791:Enzyme Inhibitors; D005227:Fatty Acids; D064801:Phospholipase A2 Inhibitors; D010936:Plant Extracts; D011994:Recombinant Proteins