Two-step purification of low-content cellular protein using protein-imprinted polymers.
We introduce a new method, based on molecular imprinting, for purification of low-content cellular protein. This is a combination method that uses two types of protein-imprinting polymers (PIPs) synthesized with limited-length polymer chains that contain randomly distributed recognition sites, namely assistant recognition polymer chains, and uses cloned bacterial protein as a template. The low-content cellular target protein was purified from cell extract by this method. This is believed to be the first time that low-content cellular protein has been purified by using PIPs and with only two steps.
Long
Yi
Y
Biochemical Section of Key Laboratory of Functional Polymer Materials, the Ministry of Education of China, Institute of Polymer Chemistry, Nankai University, 300071 Tianjin, People's Republic of China.
Xing
Xiaocui
X
Han
Ruifang
R
Sun
Yang
Y
Wang
Ying
Y
Zhao
Zhuo
Z
Mi
Huaifeng
H
D000818:Animals; D002457:Cell Extracts / Q000032:analysis*; D008099:Liver / Q000737:chemistry; D008863:Microspheres; D054802:Molecular Imprinting / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000138:chemical synthesis / Q000737:chemistry; D011506:Proteins / Q000302:isolation & purification*; D012333:RNA, Messenger / Q000737:chemistry / Q000235:genetics; D013552:Swine
D002457:Cell Extracts; D011108:Polymers; D011506:Proteins; D012333:RNA, Messenger; D011092:Polyethylene Glycols; D011142:Polyvinyl Alcohol