A photoactivatable push-pull fluorophore for single-molecule imaging in live cells.
We have reengineered a red-emitting dicyanomethylenedihydrofuran push-pull fluorophore so that it is dark until photoactivated with a short burst of low-intensity violet light. Photoactivation of the dark fluorogen leads to conversion of an azide to an amine, which shifts the absorption to long wavelengths. After photoactivation, the fluorophore is bright and photostable enough to be imaged on the single-molecule level in living cells. This proof-of-principle demonstration provides a new class of bright photoactivatable fluorophores, as are needed for super-resolution imaging schemes that require active control of single molecule emission.
Lord
Samuel J
SJ
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Conley
Nicholas R
NR
Lee
Hsiao-lu D
HL
Samuel
Reichel
R
Liu
Na
N
Twieg
Robert J
RJ
Moerner
W E
WE
D000818:Animals; D001386:Azides / Q000138:chemical synthesis / Q000737:chemistry; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D005663:Furans / Q000138:chemical synthesis / Q000737:chemistry*; D009570:Nitriles / Q000138:chemical synthesis / Q000737:chemistry*; D010777:Photochemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
D001386:Azides; D005456:Fluorescent Dyes; D005663:Furans; D009570:Nitriles; C521333:dicyanomethylenedihydrofuran