[An investigation of the protective effect of prostaglandin E1 liposome on acute lung injury in pig].
OBJECTIVE
To assess the protective effect of prostaglandin E1 liposome (lipo-PGE1) on acute lung injury (ALI) in a porcine model of ALI.


METHODS
The ALI model was reproduced by lipopolysaccharide (LPS) intravenous instillation and high tidal volume ventilation. A total of 16 domestic pigs were randomized to receive lipo-PGE1 (n=8) or placebo (n=8). Parameters of haemodynamics and pulmonary gas exchange were monitored through pulmonary artery catheter and blood gas analysis at baseline and 2, 3, 4 and 5 hours after LPS instillation. The inflation quasi-static pressure-volume (P-V) curve was obtained by ventilator occlusion technique, and the P-V data sets were fit with sigmoidal equation in order to define and compare the respiratory mechanics in animals of two groups. Plasma levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8) were determined by enzyme-linked immunosorbent assay (ELISA).


RESULTS
There was a decrease in cardiac index (CI) and mean arterial pressure (MAP) in control group compared with those in the group who received lipo-PGE1. In the group receiving lipo-PGE1 the parameter of oxygenation, including partial pressure of oxygen in arterial blood (PaO(2)), oxygenation index (PaO(2)/FiO(2)), and alveolar-arterial oxygen difference (A-aDO(2)) were significantly improved (all P<0.05) and pulmonary shunt (Qs/Qt) associated with lung injury was also significantly reduced (P<0.05) compared with control group. The respiratory mechanics (including lower and upper inflection point) in the group given lipo-PGE1 were better than those of control group (all P<0.05). Plasma levels of TNF-alpha and IL-8 were found to rise in both groups, but the rise in TNF-alpha and IL-8 in the group in which lipo-PGE1 was given were lower with shorter period compared with control group(all P<0.05).


CONCLUSIONS
Intravenous delivery of lipo-PGE1 significantly attenuate ALI caused by LPS instillation and high tidal ventilation, and it also shows beneficial effects on haemodynamics.
Intensive Care Unit, Affiliated Hospital of Guilin Medical College, Guilin 541001, Guangxi, China.
Jun
J
Xiao
Rong
R
Zhong
Shan-Yang
SY
Shi
Gui-Shan
GS
Ye
Bing-Jie
BJ
Xia
Chang-Ming
CM
Wang
Wei
W
Huang
D055371:Acute Lung Injury / Q000097:blood / Q000473:pathology / Q000517:prevention & control*; D000527:Alprostadil / Q000008:administration & dosage*; D000818:Animals; D004195:Disease Models, Animal; D016209:Interleukin-8 / Q000097:blood; D008168:Lung / Q000473:pathology; D011897:Random Allocation; D034421:Sus scrofa; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D000527:Alprostadil