Determination of Fumonisin B1 in animal tissues with immunoaffinity purification.
Immunoaffinity extraction combined with high-performance liquid chromatography (HPLC) with fluorescence detection was developed to determine Fumonisin B1 (FB1) in duck tissues. The method was linear over a concentration range of 0.013-0.250 microg of FB1/g of liver, kidney and muscle. The limit of quantification was 0.013 microg FB1/g in tissue. The mean percentage of extraction was 75% for liver and kidney and 53% for muscle. This method can be used in duck for the detection of FB1 contamination after exposure, the liver being the most contaminated tissue.
Unité de Mycotoxicologie, Ecole Nationale Vétérinaire de Toulouse, 23 chemin des Capelles, B.P. 87614, 31076 Toulouse cedex, France. d.tardieu@envt.fr
Didier
D
Tardieu
Aliénor
A
Auby
Caroline
C
Bluteau
Jean Denis
JD
Bailly
Philippe
P
Guerre
D000818:Animals; D002457:Cell Extracts / Q000737:chemistry; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004372:Ducks; D037341:Fumonisins / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics; D007668:Kidney / Q000737:chemistry*; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*
D002457:Cell Extracts; D037341:Fumonisins; C056933:fumonisin B1