Effect of dithiocarbamates on cellulase activity in culture filtrates of Trichoderma reesei.
Different concentrations of tetramethylthiuram disulfide (TMTD), sodium dimethyldithiocarbamate (NaDDC), and zinc dimethyldithiocarbamate (ZnDDC) affected the amount of cellulase(s) activity in the culture of Trichoderma reesei. After eight days incubation at 28 degrees C the greatest increase in Avicelase, CMCase, and beta-glucosidase over the control were observed at 0.1 ppm (TMTD) and 0.4 ppm (NaDDC and ZnDCC). There was decrease in the growth in the ZnDDC, but beta-glucosidase activity was reduced considerably. Total protein in the culture filtrate increased with the increase in cellulase(s) activity. No change in pH was observed at eight days incubation but pH increased (not exceeding 5.9) at 12 days incubation.
Department of Microbiology, Punjab University, Chandigarh, CAZRI, Jodhpur, India.
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Shirkot
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Mann
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Dhawan
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Gupta
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Gupta