Fractionated marine invertebrate extract libraries for drug discovery.
The high-throughput screening and drug discovery paradigm has necessitated a change in preparation of natural product samples for screening programs. In an attempt to improve the quality of marine natural products samples for screening, several fractionation strategies were investigated. The final method used HP20SS as a solid support to effectively desalt extracts and fractionate the organic components. Additionally, methods to integrate an automated LCMS fractionation approach to shorten discovery time lines have been implemented.
Bugni
Tim S
TS
Department of Medicinal Chemistry, University of Utah, 30 S. 2000 E. RM 307, Salt Lake City, UT 84112, USA.
Harper
Mary Kay
MK
McCulloch
Malcolm W B
MW
Reppart
Jason
J
Ireland
Chris M
CM
D000818:Animals; D001688:Biological Products / Q000302:isolation & purification; D005591:Chemical Fractionation; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D007448:Invertebrates / Q000737:chemistry*; D054852:Small Molecule Libraries
D001688:Biological Products; D054852:Small Molecule Libraries