title: Non-enzymatic glucose biosensor based on overoxidized polypyrrole nanofiber electrode modified with cobalt(II) phthalocyanine tetrasulfonate.
abstract: An enzymeless biosensor, based on electrodeposition of overoxidized polypyrrole nanofiber onto pencil graphite electrode and modified with cobalt(II) phthalocyanine tetrasulfonate (CoPcTS), was investigated in this study. CoPcTS showed electrocatalytic activity for the oxidation of glucose in alkaline solution. The electrochemical performance of the modified electrodes was investigated by differential pulse voltammetric (DPV) method. The resulting biosensor exhibited excellent performance for glucose determination with a wide linear range (0.25-20mM), a highly reproducible response (R.S.D. of 2.7%), low percentage of the interferences and long-term stability. The calculated detection limit was 0.1mM at 3sigma. In order to verify the reliability of the biosensor, it was applied to the determination of glucose in serum samples. The results were satisfactory and agreed closely with those measured in a hospital.
authors.affiliation: Faculty of Science, Department of Chemistry, Anadolu University, Yunus Emre Campus, Tepebasi, 26470 Eskişehir, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Ozcan
authors.forename: Yücel
authors.initials: Y
authors.lastname: Sahin
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Türk
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D001786:Blood Glucose / Q000032:analysis*; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D019544:Equipment Failure Analysis; D007211:Indoles / Q000737:chemistry*; D008839:Microelectrodes; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D036103:Nanotechnology / Q000295:instrumentation* / Q000379:methods; D009942:Organometallic Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D001786:Blood Glucose; D020099:Coated Materials, Biocompatible; D007211:Indoles; D009942:Organometallic Compounds; D011108:Polymers; D011758:Pyrroles; C052738:cobalt tetrasulfophthalocyanine; C067635:polypyrrole; D010088:Oxidoreductases