Stereoselective bioreduction of bulky-bulky ketones by a novel ADH from Ralstonia sp.
Ketones with two bulky substituents, named bulky-bulky ketones, as well as less sterically demanding ketones were successfully reduced to the corresponding optically highly enriched alcohols using a novel identified recombinant short-chain alcohol dehydrogenase RasADH from Ralstonia sp. DSM 6428 overexpressed in E. coli.
Lavandera
Iván
I
Research Centre Applied Biocatalysis c/o Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria.
Kern
Alexander
A
Ferreira-Silva
Bianca
B
Glieder
Anton
A
de Wildeman
Stefaan
S
Kroutil
Wolfgang
W
D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D002384:Catalysis; D004926:Escherichia coli / Q000201:enzymology; D007659:Ketones / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D043367:Ralstonia / Q000201:enzymology*; D013379:Substrate Specificity; D018631:ras Proteins / Q000378:metabolism
D007659:Ketones; D000426:Alcohol Dehydrogenase; D018631:ras Proteins