title: Glycosylation of daidzein by the Eucalyptus cell cultures.
abstract: The sequential glycosylation of a soybean isoflavone, daidzein, with cultured suspension cells of Eucalyptus perriniana and cyclodextrin glucanotransferase was studied. Daidzein was converted into two glycosylation products, daidzein 7-O-beta-D-glucopyranoside (39%) and daidzein 7-O-[6-O-(beta-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-gentiobioside, 6%), by cultured E. perriniana cells. Further glycosylation of daidzein 7-O-beta-glucoside with cyclodextrin glucanotransferase gave daidzein 7-O-[4-O-(alpha-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-maltoside, 26%), daidzein 7-O-beta-maltotrioside (15%), and daidzein 7-O-beta-maltotetraoside (7%).
authors.affiliation: Department of Pharmacology and Therapeutics, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Shimoda
authors.forename: Noriaki
authors.initials: N
authors.lastname: Sato
authors.forename: Tatsunari
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Hatsuyuki
authors.initials: H
authors.lastname: Hamada
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hamada
mesh_terms: D002478:Cells, Cultured / Q000378:metabolism; D003505:Cyclodextrins / Q000378:metabolism*; D005052:Eucalyptus / Q000166:cytology / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D006031:Glycosylation; D007529:Isoflavones / Q000378:metabolism*; D007700:Kinetics; D010636:Phenols / Q000378:metabolism
chemical_list: D003505:Cyclodextrins; D005960:Glucosides; D007529:Isoflavones; D010636:Phenols; C004742:daidzein; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase