title: Pre-fractionated microbial samples--the second generation natural products library at Wyeth.
abstract: From the beginning of the antibiotic era in the 1940s to the present, Wyeth has sustained an active research program in the area of natural products discovery. This program has continually evolved through the years in order to best align with the "current" drug discovery paradigm in the pharmaceutical industry. The introduction of high-throughput screening and the miniaturization of assays have created a need to optimize natural product samples to better suit these new technologies. Furthermore, natural product programs are faced with an ever shortening time period from hit detection to lead characterization. To address these issues, Wyeth has created a pre-fractionated natural products library using reversed-phase HPLC to complement their existing library of crude extracts. The details of the pre-fractionated library and a cost-benefit analysis will be presented in this review.
authors.affiliation: Natural Products Discovery Research, Chemical and Screening Sciences, Wyeth Research, Pearl River, New York 10965, USA. wagenam@wyeth.com
authors.forename: Melissa M
authors.initials: MM
authors.lastname: Wagenaar
mesh_terms: D000890:Anti-Infective Agents / Q000302:isolation & purification*; D001688:Biological Products / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid; D054852:Small Molecule Libraries; D013678:Technology, Pharmaceutical / Q000379:methods*
chemical_list: D000890:Anti-Infective Agents; D001688:Biological Products; D054852:Small Molecule Libraries