Characterization of porcine ASB6 gene and transcripts-comparison of mammalian transcripts.
A member of the porcine Ankyrin repeat and suppressor of cytokine signaling (SOCS) Box protein family (ASB), designed as ASB6, was sequenced and the genomic organization of the six exons was determined. We present here a detailed analysis of ASB6 transcripts in pigs. We demonstrate the existence of an alternative transcript resulting from intron retention. This secondary transcript, if functional, encodes a protein without SOCS box. A comparison of mammalian ASB6 transcripts is performed to demonstrate the importance of transcripts encoding for a truncated ASB6 protein.
Robic
Annie
A
Institut National de la Recherche Agronomique (INRA), Laboratoire de Genetique Cellulaire, Castanet Tolosan Cedex, France. annie.robic@toulouse.inra.fr
Faraut
Thomas
T
Liaubet
Laurence
L
Riquet
Juliette
J
Milan
Denis
D
Lobjois
Valerie
V
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D017089:Ankyrin Repeat; D001483:Base Sequence; D002417:Cattle; D018076:DNA, Complementary / Q000302:isolation & purification; D020224:Expressed Sequence Tags; D006801:Humans; D007438:Introns; D051379:Mice; D008969:Molecular Sequence Data; D018482:Muscle, Skeletal / Q000378:metabolism; D016133:Polymerase Chain Reaction; D051381:Rats; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics*; D034421:Sus scrofa / Q000235:genetics*; D014158:Transcription, Genetic
D018076:DNA, Complementary; D050826:Suppressor of Cytokine Signaling Proteins