title: Assembly of polypeptide-functionalized gold nanoparticles through a heteroassociation- and folding-dependent bridging.
abstract: Gold nanoparticles were functionalized with a synthetic polypeptide, de novo-designed to associate with a charge complementary linker polypeptide in a folding-dependent manner. A heterotrimeric complex that folds into two disulphide-linked four-helix bundles is formed when the linker polypeptide associates with two of the immobilized peptides. The heterotrimer forms in between separate particles and induces a rapid and extensive aggregation with a well-defined interparticle spacing. The aggregated particles are redispersed when the disulphide bridge in the linker polypeptide is reduced.
authors.affiliation: Division of Sensor Science and Molecular Physics, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
authors.forename: Daniel
authors.initials: D
authors.lastname: Aili
authors.forename: Karin
authors.initials: K
authors.lastname: Enander
authors.forename: Lars
authors.initials: L
authors.lastname: Baltzer
authors.forename: Bo
authors.initials: B
authors.lastname: Liedberg
mesh_terms: D004220:Disulfides / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism*; D017510:Protein Folding; D017434:Protein Structure, Tertiary
chemical_list: D004220:Disulfides; D010455:Peptides; D006046:Gold