Molecularly imprinted polymers for RGD selective recognition and separation.
Molecularly imprinted polymers that could recognize the tripeptide Arg-Gly-Asp have been produced with the use of two functional monomers and three different cross-linkers, respectively. Methacrylic acid and acrylamide were used as functional monomers and the role of the ethylene glycol dimethacrylate, trimethylpropane trimethacrylate and N,N'-methylene-bisacrylamide as crosslinking monomers, was investigated on their recognition capability. The % net rebinding and the imprinting factor values were obtained, giving for the methacrylic acid-trimethylpropane trimethacrylate polymer the highest values 12.3% and 2.44, respectively. In addition, this polymer presented lower dissociation constant (K(D)) value and the higher B (max)% of theoretical total binding sites than all the other polymers. Rebinding experiments with Lys-Gly-Asp, an analogue of Arg-Gly-Asp, and other different peptides, such as cholecystokinin C-terminal tri- and pentapeptide and gramicidin, further indicated the selectivity of methacrylic acid-trimethylpropane trimethacrylate copolymer for Arg-Gly-Asp giving specific selectivity factor values 1.27, 1.98, 1.31 and 1.67, respectively.
Papaioannou
Emmanuel
E
Section of Chemistry, Faculty of Chemical Engineering, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Koutsas
Christos
C
Liakopoulou-Kyriakides
Maria
M
D000178:Acrylamides / Q000737:chemistry; D003432:Cross-Linking Reagents / Q000737:chemistry; D008689:Methacrylates / Q000138:chemical synthesis / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D009842:Oligopeptides / Q000737:chemistry*; D011109:Polymethacrylic Acids / Q000138:chemical synthesis / Q000737:chemistry*
D000178:Acrylamides; D003432:Cross-Linking Reagents; D008689:Methacrylates; D009842:Oligopeptides; D011109:Polymethacrylic Acids; C047981:arginyl-glycyl-aspartic acid; C004919:ethylene dimethacrylate; C021221:N,N'-methylenebisacrylamide