title: [Indication and identification of Lactobacillus spp. with the use of polymerase chain reaction].
abstract: OBJECTIVE
  The aim of the work is the development of laboratory test for indication and identification of Lactobacillus spp. by the polymerase chain reaction.
  
  
  METHODS
  The work is developed on the base of the GenBank/EMBL data about genetic sequences of the Lactobacillus spp. The sequences of DNA were studied with the help of the ClustalW program. The strains of the Lactobacillus spp., which are the object of the research, have been registered in Russian collection of industrial microorganisms.
  
  
  RESULTS
  The laboratory test of nested-PCR for indication and identification L. plantarum, L. fermentum, L. acidophilus, L. delbrueckii, L. casei, L. rhamnosus was performed. The specificity of the nested-PCR was correlated with the control analyses of monoculture Lactobacillus spp. and commercial products.
  
  
  CONCLUSIONS
  The new developed laboratory nested-PCR test may be use in control system of milk foods enriched by probiotic microorganisms.
authors.forename: A G
authors.initials: AG
authors.lastname: Tochilina
authors.forename: N A
authors.initials: NA
authors.lastname: Novikova
authors.forename: K Ia
authors.initials: KIa
authors.lastname: Sokolova
authors.forename: I V
authors.initials: IV
authors.lastname: Solov'eva
authors.forename: I V
authors.initials: IV
authors.lastname: Belova
authors.forename: T P
authors.initials: TP
authors.lastname: Ivanova
authors.forename: L B
authors.initials: LB
authors.lastname: Lukovnikova
authors.forename: L N
authors.initials: LN
authors.lastname: Golotsyna
mesh_terms: D015373:Bacterial Typing Techniques / Q000379:methods; D017931:DNA Primers; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics / Q000145:classification* / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D012984:Software
chemical_list: D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S