title: Differential expression of ubiquitin-conjugating enzyme E2r in the developing ovary and testis of penaeid shrimp Marsupenaeus japonicus.
abstract: In order to identify genes involved in oogenesis and spermatogenesis in penaeid shrimp Marsupenaeus japonicus, a modified annealing control primer (ACP) system was adapted to identify genes differentially expressed in ovary and testis at different developmental stages. By using 20 pairs of ACP primers, 8 differentially expressed genes were obtained. One of these genes is ubiquitin-conjugating enzyme E2r (UBE2r). Bioinformatics analyses show that this gene encodes a protein of 241 amino acids with a predicted molecular mass of 27.4 kDa. Real time PCR analyses demonstrated that the expression level changed significantly in the developing testis and ovary. In the stage 2 of testis, it reached its highest expression level, the lowest expression level present in the stage 1 of ovary. The significantly different expression levels in developing testis and ovary suggest that UBE2r has an important role in oogenesis and spermatogenesis. This article is the first report of UBE2r in crustaceans and also is the first report showing that UBE2r is differentially expressed at different stages of the developing ovary and testis in an animal.
authors.affiliation: The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, China.
authors.forename: Bingling
authors.initials: B
authors.lastname: Shen
authors.forename: Ziping
authors.initials: Z
authors.lastname: Zhang
authors.forename: Yilei
authors.initials: Y
authors.lastname: Wang
authors.forename: Guodong
authors.initials: G
authors.lastname: Wang
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Lin
authors.forename: Shuhong
authors.initials: S
authors.lastname: Wang
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Zou
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D020869:Gene Expression Profiling; D015971:Gene Expression Regulation, Enzymologic; D008297:Male; D008969:Molecular Sequence Data; D010053:Ovary / Q000196:embryology / Q000201:enzymology*; D033561:Penaeidae / Q000196:embryology* / Q000201:enzymology*; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013737:Testis / Q000196:embryology / Q000201:enzymology*; D044763:Ubiquitin-Conjugating Enzymes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D044763:Ubiquitin-Conjugating Enzymes