Simultaneous multifocal, multiphoton, photon counting microscopy.
We demonstrate a novel multifocal, multiphoton microscope that is capable of simultaneous dynamic imaging of multiple focal planes. We show for the first time that multimodal, multiphoton images excited with orthogonal polarizations can be acquired simultaneously in both the transmission and epi directions.
Carriles
Ramón
R
Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA. rcarrile@mines.edu
Sheetz
Kraig E
KE
Hoover
Erich E
EE
Squier
Jeff A
JA
Barzda
Virginijus
V
D000465:Algorithms; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008027:Light; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics; D017785:Photons; D012815:Signal Processing, Computer-Assisted; D013997:Time Factors