Prediction of the profiles of the elution bands of proteins in preparative liquid chromatography.
A semiequilibrium model of chromatography has been developed to account for the elution profile of high concentration bands. The use of this model requires the prior determination of the equilibrium isotherms of the studied compounds in the chromatographic phase system. The model is applied here to a study of the isocratic elution bands of chicken albumin and conalbumin on a weak anion exchanger in aqueous buffered solutions. Satisfactory agreement is observed between the experimental elution profiles of large-sample-size bands of chicken albumin and conalbumin.
Katti
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A
Department of Chemical Engineering, University of Tennessee, Knoxville, Tennessee 37996-1600, USA.
Huang
J X
JX
Guiochon
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G