Protein kinase CK2 and cell polarity.
There is increasing evidence that protein kinase CK2 is involved, among a wide variety of cellular processes, in the maintenance of mammalian cell morphology and cell polarity. Here, we show that in epithelial cells, a fraction of CK2 is associated to the plasma membrane and that this localization is controlled by cell-matrix interactions. In addition, inhibition of CK2 activity in mammary epithelial cells (MCF10A), using either the specific CK2 inhibitor TBB or siRNA-mediated CK2beta knockdown, induced differential phenotypes revealing an important role of this enzyme in epithelial cell morphology.
Deshière
Alexandre
A
CEA, DSV, iRTSV, Laboratoire Transduction du signal, 17, rue des Martyrs, Grenoble, 38054, France.
Theis-Febvre
Nathalie
N
Martel
Véronique
V
Cochet
Claude
C
Filhol
Odile
O
D000818:Animals; D047390:Casein Kinase II / Q000037:antagonists & inhibitors / Q000378:metabolism*; D002460:Cell Line; D002462:Cell Membrane / Q000187:drug effects / Q000201:enzymology; D016764:Cell Polarity / Q000187:drug effects; D004285:Dogs; D004847:Epithelial Cells / Q000166:cytology* / Q000187:drug effects / Q000201:enzymology*; D006801:Humans; D051379:Mice; D010641:Phenotype; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D021122:Protein Subunits / Q000378:metabolism; D021381:Protein Transport / Q000187:drug effects; D013347:Subcellular Fractions / Q000187:drug effects / Q000201:enzymology
D047428:Protein Kinase Inhibitors; D021122:Protein Subunits; D047390:Casein Kinase II