Oligogalacturonans production by free radical depolymerization of polygalacturonan.
The large production of acidic oligosaccharides was investigated by non-enzymatic depolymerization of polygalacturonic acid (PGA) using free hydroxyl radical hydrolysis process from H2O2/copper (II) system. A large amount of oligogalacturonides (OGAs) with degrees of polymerization up to 6 were fractionated, and characterized by ESI-Q/TOF-MASS spectrometry and 1H NMR spectroscopy. This efficient production of uronic oligosaccharides from PGA constitutes an original process to produce bioactive compounds in large scale up.
Elboutachfaiti
R
R
Laboratoire des Polysaccharides Microbiens et Végétaux, IUT/GB, UPJV, Avenue des Facultés, Le Bailly, 80025 Amiens Cedex, France.
Delattre
C
C
Michaud
P
P
Courtois
B
B
Courtois
J
J
D017665:Hydroxyl Radical / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D009844:Oligosaccharides / Q000737:chemistry* / Q000302:isolation & purification; D010368:Pectins / Q000737:chemistry*; D010944:Plants / Q000737:chemistry
D009844:Oligosaccharides; C412763:oligogalacturonic acid; D017665:Hydroxyl Radical; D010368:Pectins; C003181:polygalacturonic acid