Plasma insulin concentrations during a hyperinsulinaemic clamp: what do we measure?
BACKGROUND
The aim of this study is to compare the performance of three commonly used insulin assays with respect to the detection of exogenous human and porcine insulin added to human or rat plasma.


METHODS
The DPC Immulite human insulin assay, the Mercodia rat insulin enzyme-linked immunosorbent assay and the Linco rat insulin radioimmunoassay were tested.


RESULTS
The mean cross-reactivity of exogenous insulin ranged from 25% to 92%. The mean cross-reactivity of Actrapid in human plasma on the DPC Immulite was 56% and was independent of the endogenous insulin concentration.


CONCLUSIONS
The measurement of exogenous insulin varies according to the source of exogenous insulin, matrix and insulin assay.
Ackermans
M T
MT
Laboratory of Endocrinology, Department of Clinical Chemistry, University of Amsterdam. m.t.ackermans@amc.uva.nl
Klieverik
L P
LP
Endert
E
E
Sauerwein
H P
HP
Kalsbeek
A
A
Fliers
E
E
D000818:Animals; D003429:Cross Reactions; D015309:Glucose Clamp Technique / Q000379:methods*; D006801:Humans; D007328:Insulin / Q000097:blood* / Q000276:immunology*; D051381:Rats; D013552:Swine
D007328:Insulin