Effect of human oviductal in vitro secretion on human sperm DNA integrity.
OBJECTIVE
In the female genital tract spermatozoa interact with the oviductal secretion. The aim of the study was to evaluate the effect of conditioned media (CM) from cultures of human oviductal tissue, on sperm DNA integrity. The effect of H(2)O(2) on sperm DNA integrity, before and after incubation under capacitating conditions, was also investigated.


METHODS
Motile sperm obtained from normozoospermic semen samples were incubated (4 h or 22 h) in the presence or absence of CM and further exposed to H(2)O(2). DNA damage was detected by the comet assay.


RESULTS
The CM significantly reduced the DNA damage associated with sperm incubation, and also decreased the effect of H(2)O(2) after 4 h incubation, compared to controls. The H(2)O(2) caused a dose-dependent deleterious effect on sperm DNA integrity both before and following 22 h of capacitation.


CONCLUSIONS
The oviductal tissue CM increased the stabilization of the sperm DNA structure under culture conditions.
Robert
Celeste
C
Laboratory of Reproductive Studies, Area of Clinical Biochemistry, School of Biochemical and Pharmaceutical Sciences, National University of Rosario, Rosario, Argentina.
Caille
Adriana
A
Zumoffen
Carlos
C
Cabada
Marcelo
M
Ghersevich
Sergio
S
D000328:Adult; D000465:Algorithms; D001826:Body Fluids / Q000378:metabolism / Q000502:physiology*; D002470:Cell Survival / Q000187:drug effects; D020552:Comet Assay; D004247:DNA / Q000187:drug effects / Q000378:metabolism / Q000502:physiology; D004249:DNA Damage / Q000502:physiology; D005187:Fallopian Tubes / Q000378:metabolism*; D005260:Female; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D008875:Middle Aged; D013075:Sperm Capacitation / Q000187:drug effects / Q000502:physiology; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects / Q000378:metabolism* / Q000502:physiology
D004247:DNA; D006861:Hydrogen Peroxide