title: Calcium-supplemented University of Wisconsin solution in long-term myocardial preservation.
abstract: The purpose of this study was to assess the effects of the addition of calcium to University of Wisconsin solution in long-term myocardial perfusion. In a heterotopic heart transplantation model, performed in pigs, the donor heart was preserved for 24 hours by means of continuous perfusion in this solution, without (24hUW group) or with calcium, 2.4 mmol/L (24hUW+Ca). During this period, the oxygenation and pH of the solution were measured, as were the calcium and lactate concentrations and enzyme release. After two hours of reperfusion, samples were collected from both ventricles for the morphological study. In the control group, there were no signs that reperfusion had triggered the calcium paradox. The addition of this cation to the preservation solution improved the intercellular junction integrity but, at the same time, favored intracellular calcium overload. This is manifested by increased enzyme release during preservation (LDH: 242+/-95 vs 140+/-25; CK: 668+/-371 vs 299+/-83 (U/L). p<0.01 in both cases) and signs of ventricular contracture: hardness and stiffness were significantly more prominent than in the group without calcium supplementation. Moreover, in comparison with the control group, the structural morphology of 24hUW+Ca is characterized by the more prominent and extensive presence of contraction bands and disorganized actin structure. Thus, under the experimental conditions employed in this study, we consider the addition of calcium to Wisconsin solution to be unadvisable.
authors.affiliation: Department of Experimental Surgery, Puerta de Hierro University Hospital, Madrid, Spain. lalvarez.hpth@salud.madrid.org
authors.forename: Lourdes
authors.initials: L
authors.lastname: Alvarez-Ayuso
authors.forename: Soledad
authors.initials: S
authors.lastname: García Gómez-Heras
authors.forename: Jorge R
authors.initials: JR
authors.lastname: Roda
authors.forename: Eduardo
authors.initials: E
authors.lastname: Jorge
authors.forename: Patricia
authors.initials: P
authors.lastname: Calero
authors.forename: Amalia
authors.initials: A
authors.lastname: Torralba
authors.forename: Isabel
authors.initials: I
authors.lastname: Millán
authors.forename: Héctor
authors.initials: H
authors.lastname: Fernández-García
authors.forename: Eduardo
authors.initials: E
authors.lastname: García-Poblete
mesh_terms: D000199:Actins / Q000378:metabolism; D000241:Adenosine / Q000627:therapeutic use; D000493:Allopurinol / Q000627:therapeutic use; D000818:Animals; D002118:Calcium / Q000627:therapeutic use*; D002314:Cardioplegic Solutions / Q000627:therapeutic use*; D015925:Cryopreservation / Q000379:methods*; D005978:Glutathione / Q000627:therapeutic use; D006321:Heart; D016027:Heart Transplantation; D006352:Heart Ventricles / Q000187:drug effects / Q000473:pathology; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000627:therapeutic use; D007365:Intercellular Junctions / Q000000981:diagnostic imaging / Q000187:drug effects; D015425:Myocardial Reperfusion; D009206:Myocardium / Q000378:metabolism / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000648:ultrastructure; D009926:Organ Preservation / Q000379:methods*; D019188:Organ Preservation Solutions / Q000627:therapeutic use*; D010100:Oxygen / Q000032:analysis; D011887:Raffinose / Q000627:therapeutic use; D013552:Swine; D020858:Tissue and Organ Harvesting; D014463:Ultrasonography
chemical_list: D000199:Actins; D002314:Cardioplegic Solutions; D007328:Insulin; D019188:Organ Preservation Solutions; C055206:University of Wisconsin-lactobionate solution; D000493:Allopurinol; D005978:Glutathione; D000241:Adenosine; D011887:Raffinose; D010100:Oxygen; D002118:Calcium