Low-pH-induced membrane fusion mediated by human metapneumovirus F protein is a rare, strain-dependent phenomenon.
Membrane fusion promoted by human metapneumovirus (HMPV) fusion (F) protein was suggested to require low pH (R. M. Schowalter, S. E. Smith, and R. E. Dutch, J. Virol. 80:10931-10941, 2006). Using prototype F proteins representing the four HMPV genetic lineages, we detected low-pH-dependent fusion only with some lineage A proteins and not with lineage B proteins. A glycine at position 294 was found responsible for the low-pH requirement in lineage A proteins. Only 6% of all HMPV lineage A F sequences have 294G, and none of the lineage B sequences have 294G. Thus, acidic pH is not a general trigger of HMPV F proteins for activity.
Herfst
Sander
S
Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.
Mas
Vicente
V
Ver
Lorena S
LS
Wierda
Rutger J
RJ
Osterhaus
Albert D M E
AD
Fouchier
Ron A M
RA
Melero
José A
JA
D000818:Animals; D002522:Chlorocebus aethiops; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008561:Membrane Fusion / Q000235:genetics / Q000502:physiology*; D029121:Metapneumovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D008958:Models, Molecular; D010802:Phylogeny; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D014709:Vero Cells; D014760:Viral Fusion Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
D014760:Viral Fusion Proteins