title: Optimization of physiological factors for direct saccharification of cassava starch to glucose by Rhizopus oligosporus 145f.
abstract: Direct saccharification of 2.64% cassava starch by Rhizopus oligosporus 145F was attempted under various cultural conditions. Maximum glucose yield of 18.0 g/L culture filtrate was obtained with an initial pH 3.8, 2% (v/v) inoculum of R. oligosporus spores, and an incubation temperature of 45 degrees C in shake flask cultures for 48 h. This concomitantly produced 2.7 g mycelia/100g cassava starch containing 20.2% true protein. The production of glucose and mycelia was accomplished with 92.8% starch saccharification having 67.9% starch to glucose conversion efficiency.
authors.affiliation: Department of Microbiology, University of Queensland, St. Lucia, Brisbane, Queensland, Australia 4067.
authors.forename: S K
authors.initials: SK
authors.lastname: Garg
authors.forename: H W
authors.initials: HW
authors.lastname: Doelle