title: Electrospinning of bovine serum albumin. Optimization and the use for production of biosensors.
abstract: Electrospinning of the globular protein, bovine serum albumin (BSA), was optimized to obtain proteinous fibers suitable as biosensors. It was shown that the as-spun protein preserves its native form, whereas solubility of the cross-linked in the ambient conditions BSA nanofibers evidently decreases. Insoluble BSA fibers can be easily modified to be used as two-dimensional biosensors. Here, we show the micro pH sensor obtained from the BSA fiber stained with a fluorescein derivative (FITC).
authors.affiliation: Institute of Fundamental Technological Research, Polish Academy of Sciences, IPPT PAN, Swietokrzyska 21, Warszawa, 00-049, Poland. tomasz.kowalczyk@ippt.gov.pl
authors.forename: Tomasz
authors.initials: T
authors.lastname: Kowalczyk
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Nowicka
authors.forename: Danek
authors.initials: D
authors.lastname: Elbaum
authors.forename: Tomasz A
authors.initials: TA
authors.lastname: Kowalewski
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods*; D002417:Cattle; D016650:Fluorescein-5-isothiocyanate; D049329:Nanostructures; D011487:Protein Conformation; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate