[Effects of intense pulse light on the mRNA expression of type I procollagen in human fibroblasts].
OBJECTIVE
To investigate the effects of intense pulse light (IPL) on the mRNA expression of type I procollagen in human fibroblasts.


METHODS
Four healthy female subjects aged 19 - 41, underwent IPL on the back skin 3 times with an interval of 2 weeks. Before the IPL and after 1, 2, and 3 times of treatment samples of back skin were obtained to undergo real time quantitative PCR to detect the mRNA expression of type I procollagen.


RESULTS
The mRNA expression level of type I procollagen after one time of IPL treatment was [(4.7 +/- 1.0) x 10(-4)], not significantly different from that before treatment [(2.1 +/- 0.7) x 10(-4), P = 0.100]. The mRNA expression levels of type I procollagen after treatment for 2 times and 3 -time were (7.9 +/- 1.7) x 10(-4) and (11.1 +/- 2.4) x 10(-4) respectively, both significantly higher than that before treatment (both P < 0.05).


CONCLUSIONS
Promoting the mRNA transcription of type I procollagen in dermal fibroblasts, IPL therapy is effective in dermal remodeling and wrinkle removing.
Mei
Xue-Ling
XL
Department of Dermatology, Beijing Friendship Hospital Affiliated, Capital University of Medical Sciences, Beijing 100050, China.
Zhao
Jun-Ying
JY
Cui
Yan
Y
D000328:Adult; D024042:Collagen Type I / Q000235:genetics*; D005260:Female; D005347:Fibroblasts / Q000378:metabolism* / Q000528:radiation effects*; D015870:Gene Expression / Q000528:radiation effects; D006801:Humans; D010789:Phototherapy / Q000379:methods; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000378:metabolism / Q000528:radiation effects; D055815:Young Adult
D024042:Collagen Type I; D012333:RNA, Messenger