title: Identification and characterization of Bacillus anthracis spores by multiparameter flow cytometry.
abstract: In response to the need for methods that can rapidly detect potentially virulent Bacillus anthracis spores, we developed a two-color flow cytometric assay capable of simultaneously identifying B. anthracis spores and the presence of spore-associated protective antigen, a virulence marker for strains harboring the pXO1 plasmid.
authors.affiliation: Department of Chemistry, Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: William C
authors.initials: WC
authors.lastname: Schumacher
authors.forename: Craig A
authors.initials: CA
authors.lastname: Storozuk
authors.forename: Prabir K
authors.initials: PK
authors.lastname: Dutta
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Phipps
mesh_terms: D000942:Antigens, Bacterial / Q000032:analysis*; D001408:Bacillus anthracis / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D010957:Plasmids; D013171:Spores, Bacterial / Q000302:isolation & purification
chemical_list: D000942:Antigens, Bacterial; D001427:Bacterial Toxins; C030325:anthrax toxin