title: Immunohistochemical staining of Reg IV and claudin-18 is useful in the diagnosis of gastrointestinal signet ring cell carcinoma.
abstract: Signet-ring cell carcinoma (SRCC) is a unique subtype of adenocarcinoma that is characterized by abundant intracellular mucin accumulation and a crescent-shaped nucleus displaced toward one end of the cell. Identification of an SRCC's primary site is important for better planning of patient management because the treatment and prognosis differs markedly depending on the origin of the SRCC. In the present study, we analyzed the immunohistochemical characteristics of 94 cases of SRCC, including 21 cases of gastric SRCC, 16 of colorectal SRCC, 10 of breast SRCC, and 47 of pulmonary SRCC, with antibodies against Reg IV and claudin-18, which we previously identified as gastric cancer-related genes. We also tested known markers cytokeratin 7, cytokeratin 20, MUC2, MUC5AC, caudal-related homeobox gene 2 (CDX2), thyroid transcription factor-1, mammaglobin, gross cystic disease fluid protein 15, and estrogen receptor. All 21 cases of gastric SRCC and 16 cases of colorectal SRCC were positive for Reg IV, and the remaining SRCCs were negative. Eighteen of 21 (86%) gastric SRCCs and 6 of 16 (38%) colorectal SRCCs were positive for claudin-18, whereas another SRCCs were negative. In conclusion, Reg IV staining and claudin-18 staining can aid in diagnosis of gastrointestinal SRCC.
authors.affiliation: Department of Molecular Pathology, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Sentani
authors.forename: Naohide
authors.initials: N
authors.lastname: Oue
authors.forename: Takashi
authors.initials: T
authors.lastname: Tashiro
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishisaka
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Fukuhara
authors.forename: Kiyomi
authors.initials: K
authors.lastname: Taniyama
authors.forename: Hiroo
authors.initials: H
authors.lastname: Matsuura
authors.forename: Koji
authors.initials: K
authors.lastname: Arihiro
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ochiai
authors.forename: Wataru
authors.initials: W
authors.lastname: Yasui
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis*; D001943:Breast Neoplasms / Q000737:chemistry; D018279:Carcinoma, Signet Ring Cell / Q000737:chemistry* / Q000473:pathology; D057167:Claudins; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000473:pathology; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D037181:Lectins, C-Type / Q000032:analysis*; D008175:Lung Neoplasms / Q000737:chemistry; D008565:Membrane Proteins / Q000032:analysis*; D000073718:Pancreatitis-Associated Proteins; D011237:Predictive Value of Tests; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000473:pathology
chemical_list: D014408:Biomarkers, Tumor; C486683:CLDN18 protein, human; D057167:Claudins; D037181:Lectins, C-Type; D008565:Membrane Proteins; D000073718:Pancreatitis-Associated Proteins; C428528:REG4 protein, human