Protective effects of vitamin E against atrazine-induced genotoxicity in rats.
Atrazine (2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine) is one of the most commonly used herbicides to control grasses and weeds. The widespread contamination and persistence of atrazine residues in the environment has resulted in human exposure. Vitamin E is a primary antioxidant that plays an important role in protecting cells against toxicity by inactivating free radicals generated following pesticides exposure. The present study was undertaken to investigate the protective effect of vitamin E against atrazine-induced genotoxicity. Three different methods: gel electrophoresis, comet assay and micronucleus test were used to assess the atrazine-induced genotoxicity and to evaluate the protective effects of vitamin E. Atrazine was administered to male rats at a dose of 300 mg/kg body weight for a period of 7, 14 and 21 days. There was a significant increase (P<0.001) in tail length of comets from blood and liver cells treated with atrazine as compared to controls. Co-administration of vitamin E (100 mg/kg body weight) along with atrazine resulted in decrease in tail length of comets as compared to the group treated with atrazine alone. Micronucleus assay revealed a significant increase (P<0.001) in the frequency of micronucleated cells (MNCs) following atrazine administration. In the animals administrated vitamin E along with atrazine there was a significant decrease in percentage of micronuclei as compared to atrazine treated rats. The increase in frequency of micronuclei in liver cells and tail length of comets confirm genotoxicity induced by atrazine in blood and liver cells. In addition, the findings clearly demonstrate protective effect of vitamin E in attenuating atrazine-induced DNA damage.
Singh
Mohan
M
Department of Biochemistry, Panjab University, Chandigarh 160014, India. mohansran2k@yahoo.com
Kaur
Pushpindar
P
Sandhir
Rajat
R
Kiran
Ravi
R
D000818:Animals; D001280:Atrazine / Q000633:toxicity*; D004249:DNA Damage; D004341:Drug Evaluation; D004587:Electrophoresis, Agar Gel; D015386:Hazardous Substances / Q000633:toxicity; D006540:Herbicides / Q000633:toxicity*; D008099:Liver / Q000187:drug effects; D008297:Male; D015162:Micronucleus Tests; D009153:Mutagens / Q000633:toxicity*; D051381:Rats; D014810:Vitamin E / Q000494:pharmacology*
D015386:Hazardous Substances; D006540:Herbicides; D009153:Mutagens; D014810:Vitamin E; D001280:Atrazine