Isolation of total RNA from Escherichia coli using kosmotropic Hofmeister salts.
Most of the widely used RNA isolation methods involve the use of toxic chemicals, including chaotropic salts and phenol. In an effort to solve this problem, we studied an alternative method to purify total RNA without any harmful chemicals. This method was based on silica spin columns and kosmotropic Hofmeister salts. The RNA yield was comparable to that of the commercially available RNeasy Mini Kit (Qiagen) at salt concentrations between 0.5 and 1.0 M. Furthermore, the current method allowed the isolation of small RNA molecules together with all RNA molecules longer than 200 nt.
Lee
Myoyong
M
Bio and Health Lab, Samsung Advanced Institute of Technology, Nongseo-Dong, Giheung-Gu, Yongin-Si 449-712, South Korea.
Huh
Nam
N
Kim
Joon-Ho
JH
D004586:Electrophoresis; D004926:Escherichia coli / Q000737:chemistry*; D008967:Molecular Biology / Q000379:methods*; D012329:RNA, Bacterial / Q000032:analysis / Q000302:isolation & purification*; D011933:Reagent Kits, Diagnostic; D012492:Salts / Q000378:metabolism*
D012329:RNA, Bacterial; D011933:Reagent Kits, Diagnostic; D012492:Salts