3.6 kb genomic sequence from Takifugu capable of promoting axon growth-associated gene expression in developing and regenerating zebrafish neurons.
Unlike mammals, fish have the capacity for functional adult CNS regeneration, which is due, in part, to their ability to express axon growth-related genes in response to nerve injury. One such axon growth-associated gene is gap43, which is expressed during periods of developmental and regenerative axon growth, but is not expressed in CNS neurons that do not regenerate in adult mammals. We previously demonstrated that cis-regulatory elements of gap43 that are sufficient for developmental expression are not sufficient for regenerative expression in the zebrafish. Here we have identified a 3.6kb genomic sequence from Fugu rubripes that can promote reporter gene expression in the nervous system during both development and regeneration in zebrafish. This compact sequence is advantageous for functional dissection of regions important for axon growth-associated gene expression during development and/or regeneration. In addition, this sequence will also be useful for targeting gene expression to neurons during periods of growth and plasticity.
Udvadia
Ava J
AJ
University of Wisconsin - Milwaukee, Department of Biological Sciences, Great Lakes WATER Institute UWM-GLWI, 600 E. Greenfield Avenue, Milwaukee, WI 53204, United States.
D000595:Amino Acid Sequence; D000818:Animals; D030801:Animals, Genetically Modified; D001369:Axons / Q000502:physiology*; D004742:Enhancer Elements, Genetic; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D019922:GAP-43 Protein / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D016678:Genome; D008969:Molecular Sequence Data; D009416:Nerve Regeneration / Q000235:genetics*; D009420:Nervous System / Q000196:embryology* / Q000378:metabolism; D009474:Neurons / Q000378:metabolism*; D011401:Promoter Regions, Genetic; D012160:Retina / Q000196:embryology / Q000502:physiology; D017386:Sequence Homology, Amino Acid; D023741:Takifugu / Q000235:genetics*; D015027:Zebrafish / Q000196:embryology / Q000235:genetics / Q000378:metabolism
D029941:Fish Proteins; D019922:GAP-43 Protein