Determination of volatile metabolites originating from mould growth on wall paper and synthetic media.
Microbial volatile organic compounds (MVOCs) emitted from the mould species Penicillium expansum, P. chrysogenum, Aspergillus versicolor, A. fumigatus, A. niger and Cladosporium cladosporoides were analyzed by means of solid phase microextraction (SPME) and GCMS. The mould species were cultivated on the synthetic agar dichloran chloramphenicol (DG 18) and on wet wall paper. The production of MVOCs was monitored over several weeks to detect changes in the emission rates between the initial stage and later periods of growth. The cultivation on the synthetic agar resulted in MVOC patterns with a wide variety of signals. In contrast, the growth on wet wall paper led to changed MVOC patterns with less signals. The emission rates were drastically reduced. Components emitted by all six fungi species on wall paper were 2-pentanol and 2-pentanone. 1-Octen-3-ol was emitted by five fungi species. 2-Pentanol was only detected in considerable amounts during the first days of growth whereas 1-octen-3-ol had a more constant emission rate over the whole period of growth. On the basis of our studies some MVOCs could be proposed as specific for single fungi on wall paper, e.g. 1,3-dimethoxybenzene for A. versicolor and 2,4-pentandione for A. fumigatus.
Matysik
Silke
S
Department of Human Exposure Research and Epidemiology, Helmholtz Centre of Environmental Research, UFZ, Permoserstrasse 15, 04318, Leipzig, Germany. silke.matysik@ufz.de
Herbarth
Olf
O
Mueller
Andrea
A
D000362:Agar; D016902:Air Pollution, Indoor / Q000032:analysis; D001230:Aspergillus / Q000145:classification / Q000254:growth & development / Q000378:metabolism; D002963:Cladosporium / Q000145:classification / Q000254:growth & development / Q000378:metabolism; D003470:Culture Media; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods; D006798:Housing; D003904:Mitosporic Fungi / Q000145:classification / Q000254:growth & development* / Q000378:metabolism*; D009930:Organic Chemicals / Q000032:analysis* / Q000378:metabolism; D010209:Paper; D010407:Penicillium / Q000145:classification / Q000254:growth & development / Q000378:metabolism; D014835:Volatilization
D003470:Culture Media; D009930:Organic Chemicals; D000362:Agar