title: Enzymatic ligation creates discrete multinanoparticle building blocks for self-assembly.
abstract: Enzymatic ligation of discrete nanoparticle-DNA conjugates creates nanoparticle dimer and trimer structures in which the nanoparticles are linked by single-stranded DNA, rather than by double-stranded DNA as in previous experiments. Ligation was verified by agarose gel and small-angle X-ray scattering. This capability was utilized in two ways: first, to create a new class of multiparticle building blocks for nanoscale self-assembly and, second, to develop a system that can amplify a population of discrete nanoparticle assemblies.
authors.affiliation: Department of Chemistry, University of California, Berkeley, California 94720-1460, USA.
authors.forename: Shelley A
authors.initials: SA
authors.lastname: Claridge
authors.forename: Alexander J
authors.initials: AJ
authors.lastname: Mastroianni
authors.forename: Yeung B
authors.initials: YB
authors.lastname: Au
authors.forename: Huiyang W
authors.initials: HW
authors.lastname: Liang
authors.forename: Christine M
authors.initials: CM
authors.lastname: Micheel
authors.forename: Jean M J
authors.initials: JM
authors.lastname: Fréchet
authors.forename: A Paul
authors.initials: AP
authors.lastname: Alivisatos
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D018736:DNA Adducts / Q000138:chemical synthesis* / Q000737:chemistry; D011088:DNA Ligases / Q000737:chemistry*; D004277:DNA, Single-Stranded; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D018736:DNA Adducts; D004277:DNA, Single-Stranded; D006046:Gold; D011088:DNA Ligases