title: Illudalic acid as a potential LAR inhibitor: synthesis, SAR, and preliminary studies on the mechanism of action.
abstract: A novel synthesis of the human leukocyte common antigen-related (LAR) phosphatase inhibitor, illudalic acid, has been achieved by a route more amenable to structure modifications. A series of simpler analogues of illudalic acid was synthesized and evaluated for potency in inhibiting LAR. The structure-activity relationship (SAR) study has shown that the 5-formyl group and the hemi-acetal lactone are crucial for effective inhibition of LAR activity, and are the key pharmacophores of illudalic acid. The fused dimethylcyclopentene ring moiety evidently helps to enhance the potency of illudalic acid against LAR. A preliminary study of the mechanism of action of illudalic acid against LAR was conducted using electrospray ionization mass spectrometry (ESI-MS) and molecular docking techniques. The results are in full agreement with the described mechanism.
authors.affiliation: State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zuchongzhi Road, Shanghai 201203, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ling
authors.forename: Yue
authors.initials: Y
authors.lastname: Huang
authors.forename: Yueyang
authors.initials: Y
authors.lastname: Zhou
authors.forename: Zhengliang
authors.initials: Z
authors.lastname: Cai
authors.forename: Bing
authors.initials: B
authors.lastname: Xiong
authors.forename: Yahui
authors.initials: Y
authors.lastname: Zhang
authors.forename: Lanping
authors.initials: L
authors.lastname: Ma
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.forename: Jingkang
authors.initials: J
authors.lastname: Shen
mesh_terms: D003374:Coumarins / Q000737:chemistry* / Q000494:pharmacology*; D020128:Inhibitory Concentration 50; D017493:Leukocyte Common Antigens / Q000037:antagonists & inhibitors*; D008958:Models, Molecular; D015394:Molecular Structure; D021241:Spectrometry, Mass, Electrospray Ionization; D013329:Structure-Activity Relationship
chemical_list: D003374:Coumarins; D017493:Leukocyte Common Antigens; C532762:illudalic acid