Novel neutral imidazole-lipophosphoramides for transfection assays.
New helper lipids, possessing an imidazole polar head, have been synthesized and included in formulations for transfection assays; these new helper lipids can improve the transfection by a factor of up to 100 compared to the use of DOPE as co-lipid.
Mével
Mathieu
M
CEMCA, UMR CNRS 6521, Université de Bretagne Occidentale, CNRS UMR 6521, 6 Avenue Le Gorgeu, 29238 Brest, France.
Neveu
Cécile
C
Gonçalves
Cristine
C
Yaouanc
Jean-Jacques
JJ
Pichon
Chantal
C
Jaffrès
Paul-Alain
PA
Midoux
Patrick
P
D000577:Amides / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D002460:Cell Line; D015195:Drug Design; D006801:Humans; D007093:Imidazoles / Q000737:chemistry*; D008051:Lipid Bilayers / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000378:metabolism; D063088:Phosphoramides; D010756:Phosphoric Acids / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism*; D014162:Transfection / Q000379:methods*
C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D000577:Amides; D007093:Imidazoles; D008051:Lipid Bilayers; D010714:Phosphatidylethanolamines; D063088:Phosphoramides; D010756:Phosphoric Acids; C005438:phosphoramide