title: Host response to human acellular dermal matrix transplantation in a primate model of abdominal wall repair.
abstract: Commercially available human acellular dermal matrix (HADM), AlloDerm((R)), was implanted as an interpositional graft in the abdominal wall of adult vervet monkeys. Host response to implanted HADM was evaluated and compared with a human cellular dermal matrix (HCDM) and a primate acellular dermal matrix (PADM). Clinical acceptance of the acellular grafts (HADM and PADM) and graft remodeling were evidenced by fibroblast repopulation and neoangiogenesis. A mild inflammatory response marked predominantly by macrophages and T-cells was present in both HADM and PADM during the first month but was absent by 3 months. Similarly, antibody and complement deposition into the grafts as well as in the serum was evident only at the early time points. Interleukin-6 (IL-6) or IL-10 was induced in some acellular graft-implanted monkeys at the early time points, but tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), or IL-2 was not detected over the study period. In contrast, significant inflammation was observed in HCDM-implanted animals, as evidenced by immune cell infiltration (p </= 0.0001), immunoglobulin G (IgG) binding (p < 0.001), complement (C5b) deposition (p < 0.05), TNF-alpha deposition (p < 0.001), and macrophage activation (p < 0.05). Abdominal wall repair in the vervet monkey is an immunologically relevant model to evaluate functional efficacy and host immune response to implanted biomaterials and may be predictive of clinical response and surgical outcomes in humans.
authors.affiliation: LifeCell Corporation, 1 Millennium Way, Branchburg, NJ 08876, USA. hxu@lifecell.com
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Hua
authors.initials: H
authors.lastname: Wan
authors.forename: Maryellen
authors.initials: M
authors.lastname: Sandor
authors.forename: Shijie
authors.initials: S
authors.lastname: Qi
authors.forename: Frank
authors.initials: F
authors.lastname: Ervin
authors.forename: John R
authors.initials: JR
authors.lastname: Harper
authors.forename: Ronald P
authors.initials: RP
authors.lastname: Silverman
authors.forename: David J
authors.initials: DJ
authors.lastname: McQuillan
mesh_terms: D034861:Abdominal Wall / Q000473:pathology*; D000818:Animals; D000906:Antibodies; D000917:Antibody Formation / Q000276:immunology; D001672:Biocompatible Materials / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D020405:Dermis / Q000637:transplantation*; D004195:Disease Models, Animal; D006801:Humans; D007111:Immunity, Cellular / Q000276:immunology; D020007:Implants, Experimental; D007249:Inflammation; D007700:Kinetics; D008297:Male; D018919:Neovascularization, Physiologic; D011323:Primates / Q000276:immunology*; D014945:Wound Healing
chemical_list: D000906:Antibodies; D001672:Biocompatible Materials; D015415:Biomarkers