Thyroid hormone regulates renocortical COX-2 and PGE2 expression in the late gestation fetal sheep.
Cyclooxygenase 2 (COX-2) is important for development of the fetal kidney. Precisely how renal COX-2 expression is regulated in fetal life is unclear. The hypothesis that thyroid hormone positively regulates COX-2 and PGE(2) levels in the late gestation fetal kidney cortex was tested. Sham, thyroidectomized (TX), and TX + thyroid hormone replacement (R) fetal sheep were studied. TX was performed at 120 days gestational age (dGA). TX + R fetuses were continuously infused with thyroxine from 3 days after surgery until study completion. Fetal kidney cortex was obtained at 137 dGA for measurement of renal cyclooxygenase type-2 (COX-2) protein and PGE(2) metabolites. Renocortical COX-2 and PGE(2) levels were significantly lower in TX compared with sham and TX + R fetuses. There were no differences between sham and TX + R fetuses. These findings demonstrate that thyroid hormone positively regulates renal COX-2 and PGE(2) expression in the late gestation fetal sheep kidney.
Carey
Luke C
LC
Department of Obstetrics and Gynecology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA. lcarey@wfubmc.edu
Valego
Nancy K
NK
Chen
Kai
K
Rose
James C
JC
D000818:Animals; D051546:Cyclooxygenase 2 / Q000096:biosynthesis*; D015232:Dinoprostone / Q000096:biosynthesis*; D005260:Female; D005333:Fetus; D020249:Hormone Replacement Therapy; D007672:Kidney Cortex / Q000196:embryology* / Q000201:enzymology / Q000378:metabolism*; D011247:Pregnancy; D012756:Sheep; D013974:Thyroxine / Q000097:blood / Q000378:metabolism* / Q000494:pharmacology; D014284:Triiodothyronine / Q000097:blood
D014284:Triiodothyronine; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D013974:Thyroxine