title: Pediatric patients with congenital heart disease: thrombin generation measured by calibrated automated thrombography.
abstract: The aim of this study was to investigate the possible suitability of the calibrated automated thrombography to determine the coagulation status of pediatric patients with congenital heart disease. Thrombin generation was measured in 60 patients with congenital heart disease using the calibrated automated thrombography and compared to data using standard coagulation parameters such as prothrombin, antithrombin, tissue factor pathway inhibitor, prothrombin fragment 1.2 (F 1.2), and activated partial thromboplastin time. A significant positive correlation was observed between prothrombin and the endogenous thrombin potential (P < 0.01; r = 0.295) as well as between prothrombin and peak height (P < 0.01; r = 0.581). A significant negative correlation was seen between tissue factor pathway inhibitor and endogenous thrombin potential (P < 0.01; r = -0.480) and between tissue factor pathway inhibitor and peak height (P < 0.01; r = -0.234). No statistically significant correlation was found between antithrombin and parameters of continuous thrombin generation. Significant correlation was seen neither between activated partial thromboplastin time and F1.2 nor between activated partial thromboplastin time and prothrombin. The data presented here indicate that calibrated automated thrombography measurements determine thrombin generation more accurately and therefore reflect better the coagulation status of pediatric patients with congenital heart disease then standard global coagulation assays such as activated partial thromboplastin time.
authors.affiliation: Department of Pediatrics, Austria cInstitute of Physiological Chemistry, Centre of Physiological Medicine, Medical University Graz, Graz, Austria. Andrea.Rosenkranz@klinikum-graz.at
authors.forename: Andrea
authors.initials: A
authors.lastname: Rosenkranz
authors.forename: Martin
authors.initials: M
authors.lastname: Koestenberger
authors.forename: Michael
authors.initials: M
authors.lastname: Novak
authors.forename: Gerhard
authors.initials: G
authors.lastname: Cvirn
authors.forename: Bettina
authors.initials: B
authors.lastname: Leschnik
authors.forename: Andreas
authors.initials: A
authors.lastname: Gamillscheg
authors.forename: Albrecht
authors.initials: A
authors.lastname: Beitzke
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Muntean
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006330:Heart Defects, Congenital / Q000097:blood*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010314:Partial Thromboplastin Time / Q000295:instrumentation* / Q000379:methods*; D013917:Thrombin / Q000032:analysis*
chemical_list: D013917:Thrombin