Prometaphase accumulation in murine bone marrow cells following in vivo treatment with alloxan.
The aim of this study was to determine the effect of alloxan, an inhibitor of N-acetylglucosaminyl transferase that acts during the G2/M transition, on the course of mitosis in murine bone marrow cells. Mitotic cells from animals treated with different doses of alloxan were analyzed for the frequency of prometaphasic and metaphasic chromosomes based on their morphology and length. The results indicate that alloxan treatment substantially increases the frequency of prometaphase chromosomes. This suggests that N-acetylglucosaminyl transferase is also involved in the G2/M transition in bone marrow cells. Alloxan treatment also provides a method for obtaining large chromosomes for the analysis of chromosome bands, FISH and sister-chromatid exchanges.
Morales-Ramírez
P
P
Instituto Nacional de Investigaciones Nucleares, AP 18-1027 México, D.F., Mexico. pmr@nuclear.inin.mx
Rodríguez-Reyes
R
R
D000496:Alloxan / Q000494:pharmacology*; D000818:Animals; D001854:Bone Marrow Cells / Q000648:ultrastructure; D002875:Chromosomes / Q000187:drug effects; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008938:Mitosis / Q000187:drug effects*; D049468:Prometaphase / Q000187:drug effects*
D000496:Alloxan