title: Effects of medicinal mushroom (Sparassis crispa) on wound healing in streptozotocin-induced diabetic rats.
abstract: BACKGROUND
  The impaired wound healing in diabetes mellitus is a major clinical problem. Sparassis crispa (SC) is a medicinal mushroom and its beta-glucan content is more than 40%. This study investigated whether oral administration of SC could improve the impaired wound healing in diabetic rats.
  
  
  METHODS
  Full-thickness skin wounds were created on the backs of streptozotocin (STZ)-induced diabetic rats. Diabetic rats were then divided into 2 groups: SC-treated group that was orally administered doses of 1,000 mg/kg body weight per day of SC for 4 weeks and a control group without SC administration. Moreover, collagen synthesis of purified beta-glucan from SC was estimated in vitro.
  
  
  RESULTS
  Wound closure was significantly accelerated by oral administration of SC. Furthermore, in SC-treated wounds there were significant increases in macrophage and fibroblast migration, collagen regeneration, and epithelialization compared with the control group. The levels of type I collagen synthesized by cultured human dermal fibroblasts for the SC group were significantly higher than those for the control group.
  
  
  CONCLUSIONS
  SC can improve the impaired healing of diabetic wounds. This effect might involve an increase in the migration of macrophages and fibroblasts, and beta-glucan from SC directly increases the synthesis of type I collagen. Therefore, the use of SC may be extended to the clinical setting and prove an effective promoter of wound healing in patients with diabetes.
authors.affiliation: Department of Surgery, Kansai Medical University, Hisakata, Osaka, Japan. kon@takii.kmu.ac.jp
authors.forename: A-Hon
authors.initials: AH
authors.lastname: Kwon
authors.forename: Zeyu
authors.initials: Z
authors.lastname: Qiu
authors.forename: Mamiko
authors.initials: M
authors.lastname: Hashimoto
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Yamamoto
authors.forename: Takashi
authors.initials: T
authors.lastname: Kimura
mesh_terms: D000363:Agaricales; D000818:Animals; D003094:Collagen / Q000096:biosynthesis*; D003921:Diabetes Mellitus, Experimental; D004195:Disease Models, Animal; D008297:Male; D008517:Phytotherapy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing / Q000187:drug effects*; D047071:beta-Glucans / Q000378:metabolism
chemical_list: D047071:beta-Glucans; D003094:Collagen