Specificity of Arl2/Arl3 signaling is mediated by a ternary Arl3-effector-GAP complex.
Arl2 and Arl3, members of the Arf subfamily of small G proteins, are believed to be involved in ciliary and microtubule-dependent processes. Recently, we could identify RP2, responsible for a variant of X-linked retinitis pigmentosa, as the Arl3-specific GAP. Here, we have characterized Arl2/3 interactions. We show the formation of a ternary complex between Arl3, its cognate GAP RP2 and its retinal effector HRG4. This complex seems to be important for photoreceptor function.
Veltel
Stefan
S
Max-Planck-Institut für molekulare Physiologie, Abteilung Strukturelle Biologie, Otto-Hahn-Strasse 11, Dortmund, Germany.
Kravchenko
Aleksandra
A
Ismail
Shehab
S
Wittinghofer
Alfred
A
D020727:ADP-Ribosylation Factors / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing; D000595:Amino Acid Sequence; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6 / Q000378:metabolism; D005136:Eye Proteins / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000378:metabolism*; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D006153:Guanosine Diphosphate / Q000378:metabolism; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism*; D008565:Membrane Proteins / Q000378:metabolism*; D008969:Molecular Sequence Data; D020419:Photoreceptor Cells, Vertebrate / Q000201:enzymology*; D011487:Protein Conformation; D015398:Signal Transduction
D048868:Adaptor Proteins, Signal Transducing; D005136:Eye Proteins; D020690:GTPase-Activating Proteins; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; C072178:RP2 protein, human; C098032:UNC119 protein, human; D006153:Guanosine Diphosphate; C517432:PDE6D protein, human; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; C410130:ARL2 protein, human; D019204:GTP-Binding Proteins; D020727:ADP-Ribosylation Factors; C490247:ARL3 protein, human