PAHs, PAH-induced carcinogenic potency, and particle-extract-Induced cytotoxicity of traffic-related nano/ultrafine particles.
Polycyclic aromatic hydrocarbons (PAHs) bound in nano/ ultrafine particles from vehicle emissions may cause adverse health effects. However, little is known about the characteristics of the nanoparticle-bound PAHs and the PAH-associated carcinogenic potency/cytotoxicity; therefore, traffic-related nano/ultrafine particles were collected in this study using a microorifice uniform deposition impactor(MOUDI) and a nano-MOUDI. For PM0.056--18, the difference in size-distribution of particulate total-PAHs between non-after-rain and after-rain samples was statistically significant at alpha = 0.05; however, this difference was not significant for PM0.01--0.056. The PAH correlation between PM0.01--0.1 and PM0.1--1.8 was lower for the after-rain samples than forthe non-after-rain samples. The average particulate total-PAHs in five samplings displayed a trimodal distribution with a major peak in the Aitken mode (0.032--0.056 microm). About half of the particulate total-PAHs were in the ultrafine size range. The BaPeq sums of BaP, IND, and DBA (with toxic equivalence factors > or = 0.1) accounted for approximately 90% of the total-BaPeq in the nano/ultrafine particles, although these three compounds contributed little to the mass of the sampled particles. The mean content of the particle-bound total-PAHs/-BaPeqs and the PAH/BaPeq-derived carcinogenic potency followed the order nano > ultrafine > fine > coarse. For a sunny day sample, the cytotoxicity of particle extracts (using 1:1 (v/v) n-hexane/dichloromethane) was significantly higher (p < 0.05) for the nano (particularly the 10-18 nm)/ultrafine particles than for the coarser particles and bleomycin. Therefore, traffic-related nano and ultrafine particles are possibly cytotoxic.
Lin
Chih-Chung
CC
Department of Environmental Engineering and Science, National Pingtung University of Science and Technology, Nei Pu, PingTung 91201, Taiwan.
Chen
Shui-Jen
SJ
Huang
Kuo-Lin
KL
Lee
Wen-Jhy
WJ
Lin
Wen-Yinn
WY
Tsai
Jen-Hsiung
JH
Chaung
Hso-Chi
HC
D000393:Air Pollutants / Q000032:analysis* / Q000633:toxicity; D000818:Animals; D002274:Carcinogens, Environmental / Q000032:analysis* / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004784:Environmental Monitoring; D016676:Macrophages, Alveolar / Q000187:drug effects; D008297:Male; D018986:Motor Vehicles; D010316:Particle Size; D052638:Particulate Matter / Q000032:analysis* / Q000633:toxicity; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000633:toxicity; D013552:Swine; D001335:Vehicle Emissions / Q000032:analysis* / Q000633:toxicity; D014887:Weather
D000393:Air Pollutants; D002274:Carcinogens, Environmental; D052638:Particulate Matter; D011084:Polycyclic Aromatic Hydrocarbons; D001335:Vehicle Emissions