title: [Effect of medium components on callus induction and contents of polysaccharides in calli from Achyranthes bidentata].
abstract: OBJECTIVE
  To study the effect of medium components on the callus induction and the contents of polysaccharides in calli from Achyranthes bidentata.
  
  
  METHODS
  Leaves and stems were selected as explants. The effects of six kinds of factors including basal culture medium, carbon source, 2,4-D, 6-BA, TDZ, CH on the callus induction and the contents of ABPS in calli on the high growth point were studied by orthogonal design method. The data were analyzed with range analysis and variance analysis.
  
  
  RESULTS
  To leaf, the optimal medium of callus induction was B5 with 2 mg x L(-1) 2,4-D, 0.5 mg x L(-1) 6-BA, 30 g x L(-1) glucose and 1 g x L(-1) CH; to stem, the optimal one was B5 with2 mg x L(-1) 2,4-D, 1 mg x L(-1) 6-BA, 30 g x L(-1) glucose and 0.5 g x L(-1) CH. In order to obtain higher contents ABPS, to leaf calli, the optimal medium was LS with 1 mg x L(-1) 6-BA, 1 mg x L(-1) TDZ and 30 g x L(-1) sucrose; to stem calli, the optimal one was LS with 1 mg L(-1) 2,4-D, 0.5 mg x L(-1) 6-BA, 1 mg x L(-1) TDZ and 30 g x L(-1) glucose.
  
  
  CONCLUSIONS
  The optimal media of callus induction were established with stems and leaves of A. bidentata as explants and with a view to an industrial production of polysaccharides by tissue and cell culture.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang 453007, China. limingjun2002@263.net
authors.forename: Ming-Jun
authors.initials: MJ
authors.lastname: Li
authors.forename: Ping
authors.initials: P
authors.lastname: Li
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Zhang
authors.forename: Xi-Ting
authors.initials: XT
authors.lastname: Zhao
mesh_terms: D029044:Achyranthes / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism*; D018929:Cell Culture Techniques; D003470:Culture Media / Q000494:pharmacology*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D018547:Plant Stems / Q000166:cytology / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011134:Polysaccharides / Q000378:metabolism*; D046509:Tissue Culture Techniques
chemical_list: D003470:Culture Media; D004365:Drugs, Chinese Herbal; D010937:Plant Growth Regulators; D011134:Polysaccharides