Evaluation of the probiotic characteristics of newly isolated lactic acid bacteria.
Lactic acid bacteria were isolated from fermented vegetables, sour dough, milk products, sheep and human excreta. The newly isolated cultures were evaluated for a number of probiotic characteristics like bile salt resistance, salt tolerance in general, survival in low pH, hydrophobicity of the cell surface, resistance to low phenol concentration, antimicrobial activity and susceptibility pattern against vancomycin and erythromycin. The selected cultures were further screened for their ability to produce the nutraceticals such as folic acid and exopolysaccharide (EPS). Two potent isolates, CB2 (from cabbage) and SD2 (from sour dough) were found to produce both extracellular and intracellular folate. One of the isolates from yogurt (MC-1) and the one from whey (W3) produced significant amount of EPS with a maximum production of 8.79 +/- 0.05 g/l by MC-1.
Aswathy
Ravindran Girija
RG
National Institute for Interdisciplinary Science and Technology, Formerly Regional Research Laboratory, CSIR, Trivandrum, Kerala, India.
Ismail
Bindhumol
B
John
Rojan Pappy
RP
Nampoothiri
Kesavan Madhavan
KM
D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification*; D019587:Dietary Supplements; D024881:Drug Resistance, Bacterial; D005243:Feces / Q000382:microbiology; D005492:Folic Acid / Q000096:biosynthesis; D005516:Food Microbiology; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007223:Infant; D019344:Lactic Acid / Q000378:metabolism*; D008826:Microbial Sensitivity Tests; D019800:Phenol / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis; D019936:Probiotics / Q000494:pharmacology*; D055049:Salt Tolerance; D013656:Taurocholic Acid / Q000494:pharmacology
D011135:Polysaccharides, Bacterial; D019800:Phenol; D019344:Lactic Acid; D013656:Taurocholic Acid; D005492:Folic Acid