title: Effects of carbon dioxide on Penicillium chrysogenum: An autoradiographic study.
abstract: Previous research has shown that dissolved carbon dioxide causes significant changes in submerged penicillin fermentations, such as stunted, swollen hyphae, increased branching, lower growth rates, and lower penicillin productivity. Influent carbon dioxide levels of 5 and 10% were shown through the use of autoradiography to cause an increase in chitin synthesis in submerged cultures of Penicillium chrysogenum. At an influent 5% carbon dioxide level, chitin synthesis is ca. 100% greater in the subapical region of P. chrysogenum hyphae than that of the control, in which there was no influent carbon dioxide. Influent carbon dioxide of 10% caused an increase of 200% in chitin synthesis, it is believed that the cell wall must be plasticized before branching can occur and that high amounts of dissolved carbon dioxide cause the cell to lose control of the plasticizing effect, thus the severe morphological changes occur.
authors.affiliation: Department of Chemical Engineering, State University of New York at Buffalo, Buffalo, New York 14260.
authors.forename: A G
authors.initials: AG
authors.lastname: Edwards
authors.forename: C S
authors.initials: CS
authors.lastname: Ho