title: [The expression of fetuin-A and its pathological significance in fulminant hepatic failure in mice].
abstract: OBJECTIVE
  To explore the dynamic changes of fetuin-A expression and the influences of the changes on liver damage, hepatocyte apoptosis and inflammation in a mouse fulminant hepatic failure (FHF) model.
  
  
  METHODS
  The changes of fetuin-A expression were investigated by semi-quantitative RT-PCR and Western blot. Immunohistochemical staining was used in TNFa and fetuin-A detection. Hepatocyte apoptosis was detected by TUNEL.
  
  
  RESULTS
  Fetuin-A mRNA expression decreased after the FHF model was established for 3 hours (compared with the normal group, P less than 0.01), while the protein expression decreased after nine hours (compared with the normal group, P less than 0.01). Fetuin-A expressions were negatively correlated with the liver pathological scores and TNFa levels.
  
  
  CONCLUSIONS
  In our mouse FHF model, fetuin-A is a possible protective factor for liver damage.
authors.affiliation: Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Zhang
authors.forename: Xue-gong
authors.initials: XG
authors.lastname: Fan
authors.forename: Wang
authors.initials: W
authors.lastname: Haichao
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D005260:Female; D008099:Liver / Q000378:metabolism* / Q000473:pathology; D017093:Liver Failure / Q000378:metabolism* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D060749:alpha-2-HS-Glycoprotein
chemical_list: C556357:Ahsg protein, mouse; D001798:Blood Proteins; D060749:alpha-2-HS-Glycoprotein