A structured mechanistic model of the kinetics of enzymatic lysis and disruption of yeast cells.
A structured, mechanistic model has been built for the kinetics of yeast cell lysis by microbial cell lytic enzymes, based on an understanding of the two-layer yeast cell wall structure and the properties of yeast-lytic enzyme systems. The model predicts the release of protein, peptides and carbohydrates from four cell structures: the outer and inner wall layers, the cytosol and organelles or proteins present in particles; it also predicts organelle or particle lysis or solubilization and the breakdown of released proteins to peptides. Applications of the model to design and optimization of selective product release are discussed.
Hunter
J B
JB
Biochemical Engineering Laboratory, Department of Chemical Engineering and Applied Chemistry, Columbia University, New York, New York 10027.
Asenjo
J A
JA