title: Effect of ovariectomy on BMD, micro-architecture and biomechanics of cortical and cancellous bones in a sheep model.
abstract: Osteoporotic/osteopenia fractures occur most frequently in trabeculae-rich skeletal sites. The purpose of this study was to use a high-resolution micro-computed tomography (micro-CT) and dual energy X-ray absorptionmeter (DEXA) to investigate the changes in micro-architecture and bone mineral density (BMD) in a sheep model resulted from ovariectomy (OVX). Biomechanical tests were performed to evaluate the strength of the trabecular bone. Twenty adult sheeps were randomly divided into three groups: sham group (n=8), group 1 (n=4) and group 2 (n=8). In groups 1 and 2, all sheep were ovariectomized (OVX); in the sham group, the ovaries were located and the oviducts were ligated. In all animals, BMD for lumbar spine was obtained during the surgical procedure. BMD at the spine, femoral neck and femoral condyle was determined 6 months (group 1) and 12 months (group 2) post-OVX. Lumbar spines and femora were obtained and underwent BMD scan, micro-CT analysis. Compressive mechanical properties were determined from biopsies of vertebral bodies and femoral condyles. BMD, micro-architectural parameters and mechanical properties of cancellous bone did not decrease significantly at 6 months post-OVX. Twelve months after OVX, BMD, micro-architectural parameters and mechanical properties decreased significantly. The results of linear regression analyses showed that trabecular thickness (Tb.Th) (r=0.945, R2=0.886) and bone volume fraction (BV/TV) (r=0.783, R2=0.586) had strong (R2>0.5) correlation to compression stress. In OVX sheep, changes in the structural parameters of trabecular bone are comparable to the human situation during osteoporosis was induced. The sheep model presented seems to meet the criteria for an osteopenia model for fracture treatment with respect to morphometric and mechanical properties. But the duration of OVX must be longer than 12 months to ensure the animal model can be established successfully.
authors.affiliation: Institude of Orthopaedics, Xijing Hospital, the Fourth Military Medical University, No. 17 Changlexi Road, Xi'an Shaanxi Province 710032, PR China. wuzixiang@hotmail.com
authors.forename: Zi-xiang
authors.initials: ZX
authors.lastname: Wu
authors.forename: Wei
authors.initials: W
authors.lastname: Lei
authors.forename: Yun-yu
authors.initials: YY
authors.lastname: Hu
authors.forename: Hai-qiang
authors.initials: HQ
authors.lastname: Wang
authors.forename: Shi-yong
authors.initials: SY
authors.lastname: Wan
authors.forename: Zhen-sheng
authors.initials: ZS
authors.lastname: Ma
authors.forename: Hong-xun
authors.initials: HX
authors.lastname: Sang
authors.forename: Suo-chao
authors.initials: SC
authors.lastname: Fu
authors.forename: Yi-sheng
authors.initials: YS
authors.lastname: Han
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D015519:Bone Density; D001842:Bone and Bones / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D019245:Compressive Strength; D004195:Disease Models, Animal; D055119:Elastic Modulus; D005260:Female; D006801:Humans; D010024:Osteoporosis / Q000000981:diagnostic imaging* / Q000503:physiopathology*; D010052:Ovariectomy; D011859:Radiography; D012756:Sheep; D013314:Stress, Mechanical