Methyl jasmonate induces cell death with mixed characteristics of apoptosis and necrosis in cervical cancer cells.
In the present study the effectiveness of methyl jasmonate (MJ) against cervical cancer cell lines was investigated. We show that MJ is cytotoxic to a range of cervical cancer lines including SiHa, CaSki and HeLa that carry human papillomavirus (HPV) DNA and wild type p53, and C33A that is negative for HPV and contains mutant p53. Primary human foreskin keratinocytes were almost resistant to the drug. Cytotoxicity of MJ was dose and time dependent, and associated mainly with the induction of cell death and to a less extent with inhibition of cell growth. Cell death induced by MJ displayed features characteristic to both apoptosis and necrosis, and was associated with different changes in the levels of p53, p21, bcl-2 and bax in the various cervical cancer lines. In conclusion, MJ a novel anticancer agent, acts via multiple pathways to induce death of cervical cancer cells, thus making it a promising candidate for treatment of cervical cancer.
Kniazhanski
Tatiana
T
Department of Human Microbiology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
Jackman
Anna
A
Heyfets
Alina
A
Gonen
Pinhas
P
Flescher
Eliezer
E
Sherman
Levana
L
D000085:Acetates / Q000494:pharmacology*; D000255:Adenosine Triphosphate / Q000378:metabolism; D015153:Blotting, Western; D016923:Cell Death / Q000187:drug effects*; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D003517:Cyclopentanes / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005260:Female; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics; D054883:Oxylipins / Q000494:pharmacology*; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*
D000085:Acetates; D003517:Cyclopentanes; D009363:Neoplasm Proteins; D054883:Oxylipins; D000255:Adenosine Triphosphate; C072239:methyl jasmonate