title: Immobilized cell biocatalyst activation and pseudo-steady-state behavior: model and experiment.
abstract: An intrinsic, unstructured model has been utilized to describethe startup dynamics of a continuous Caalginate-immobilized Zymomonas mobilis (ATCC 10988) fermentation. This model predicts, at least qualitatively, transients in the fermenter effluent glucose, ethanol, and biomass concentrations as well as radial gradients in immobilized-cell concentration and activity within the gelbiocatalysts. Predicted intrabiocatalyst gradients in immobilized-cell specific growth rate were used to calculate the corresponding gradients in intracellular RNA level based on a reported linear relationship between the two. Mathematical simulations of immobilized biomass concentration profiles and RNA content were verified using a novel, scanning microfluorimetry technique.
authors.affiliation: Department of Chemical Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905, USA.
authors.forename: H G
authors.initials: HG
authors.lastname: Monbouquette
authors.forename: G D
authors.initials: GD
authors.lastname: Sayles
authors.forename: D F
authors.initials: DF
authors.lastname: Ollis