[Isolation and identification of the temperate bacteriophage from isolated strains of Streptococcus suis serotype 2].
A PCR assay was developed to study the distributional characteristics of phage integrase gene in Streptococcus suis serotype 2 (SS2). A 323bp distinct DNA target can be amplified in 25 strains of virulent SS2, while can not be amplified in avirulent strain T15, 5 strains of other serotypes (SS1, SS7, SS9) and strains of group C Streptococcus strains from pigs, which suggested that the phage integrase gene may be related to the pathogenicity of SS2 and can be consider as a detection factor of the virulent gene of SS2. The sequencing and restriction endonuclease analysis of the PCR products were also done. Comparisons between the sequences of phage integrase gene with that of SS2 strain, showed a high homology with SS2 China strains 98HAH33, 05ZYH33 and North American strain 89-1591. Complete cell lysis was observed with SS2 virulent strains but not with avirulent strain T15 after the induction by mitomycin C. Electron microscopy analysis of the lysate from SS2 virulent strains HA9801 and ZY05719 revealed the presence of phage particles. The induced phage, named SS2-HA and SS2-ZY, both have a small isometric nucleocapsid approximately 50 nm in diameter and have no tail and is therefore a member of the Tectiviridae family. The phage integrase gene sequence of phage SS2-HA and SS2-ZY shared high homologue identities with virulent SS2 strains, which suggested that the phage integrase gene of SS2 has high specify. The temperate phage and phage integrase gene can only detected from SS2 virulent strains but not from avirulent strain, and the detection of phage integrase gene was related to the virulence-associate factors of SS2, such as the muramidase-released protein gene (mrp), which suggested that the temperate phage of SS2 may be related to the pathogenicity of SS2.
Ma
Yuling
Y
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China. yulingm@126.com
Lu
Chengping
C
Fan
Hongjie
H
D000818:Animals; D001435:Bacteriophages / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity / Q000648:ultrastructure; D004262:DNA Restriction Enzymes / Q000378:metabolism; D016679:Genome, Viral / Q000235:genetics; D019426:Integrases / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D009696:Nucleic Acids / Q000235:genetics / Q000378:metabolism; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017011:Streptococcus suis / Q000821:virology*; D013552:Swine / Q000382:microbiology; D014771:Virion / Q000302:isolation & purification / Q000648:ultrastructure; D014774:Virulence
D009696:Nucleic Acids; D019426:Integrases; D004262:DNA Restriction Enzymes