[Effects of continuous adenosine infusion on pulmonary hypertension in chronically hypoxic rats].
OBJECTIVE
To observe the effects of continuous subcutaneous adenosine infusion on pulmonary hypertension in chronically hypoxic rats.


METHODS
Twenty-four SD rats were randomized into normoxic group, hypoxic group and adenosine-treated hypoxic group. Hypoxic environment was simulated in a chamber filled with 10% oxygen and 90% nitrogen. After 7 days of hypoxia, adenosine were administered subcutaneously in the rats in adenosine-treated group at the rate of 100 microg kg(-1) min(-1) via an Alzet micro-osmotic pump for 14 days, while the pumps in the other two groups contained normal saline. After 21 days of hypoxia, pulmonary artery pressure and tail-cuff blood pressure were measured, with the plasma rennin activity (RA), angiotensin II (AngII), endothelin (ET)-1, and nitric oxide (NO) determined. Inducible nitric oxide synthase (iNOS) expression in the pulmonary artery of the rats was detected using immunohistochemical method.


RESULTS
The mean pulmonary artery pressure (mPAP) was significantly higher in the hypoxic group than that in the normoxic group (P<0.01) and in the adenosine-treated group (P<0.01). Plasma ET-1 was significantly higher but plasma NO significantly lower in the hypoxic group than in the normoxic group (P<0.01) and the adenosine-treated group (P<0.01). iNOS expression in the pulmonary artery was higher in the hypoxic group than in normoxic group (P<0.01), and adenosine significantly increased iNOS expression in comparison with the normoxic and hypoxic groups (P<0.01). Plasma RA and AngII in the hypoxic group were significantly higher than those in the normoxic group (P<0.01) and the adenosine-treated (P<0.01).


CONCLUSIONS
Adenosine administered by continuous subcutaneous infusion alleviates chronically hypoxia-induced pulmonary hypertension in rats, in which rennin angiotensin system, ET-1, and iNOS/NO play a role.
Tan
Jian-Xin
JX
Department of Pediatrics, Clinical Division, Fuzhou General Hospital of Nanjing Command, Fuzhou 350025, China.
Chen
Xin-Min
XM
Fang
Xin
X
Tao
Meng-Jie
MJ
Huang
Xiu-Lan
XL
D000241:Adenosine / Q000008:administration & dosage / Q000627:therapeutic use*; D000818:Animals; D002908:Chronic Disease; D019332:Endothelin-1 / Q000097:blood; D006976:Hypertension, Pulmonary / Q000097:blood / Q000188:drug therapy* / Q000209:etiology; D000860:Hypoxia / Q000150:complications; D055104:Infusions, Subcutaneous; D008297:Male; D052247:Nitric Oxide Synthase Type II / Q000097:blood; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D012084:Renin-Angiotensin System / Q000187:drug effects
D019332:Endothelin-1; D052247:Nitric Oxide Synthase Type II; D000241:Adenosine