title: Influence of hydroxyethyl starch (6% HES 130/0.4) administration on hematology and clinical chemistry parameters.
abstract: BACKGROUND
  The chemical inertness of hydroxyethyl starch (HES) might cause interferences of the colloid with a variety of laboratory tests. We aimed to evaluate potential influences of HES 130/0.4, the newest HES type, on several common hematology and clinical chemistry parameters.
  
  
  RESULTS
  A convenient sample of 25 patients scheduled for rheological therapy with 500 mL 6% HES 130/0.4 was evaluated. Blood samples were drawn before and after colloid application. Comparing pre- and post-infusion values of a battery of laboratory tests (i.e., hematology and hemostasis parameters, electrolytes, enzymes, kidney and metabolic parameters, lipids, etc.) in time course, a median difference greater than the reference change value for a specific parameter was considered clinically relevant. Among all parameters tested, only serum amylase activity displayed a clinically relevant difference between pre- and post-infusion values (median increase of 85% due to HES administration). By applying in vitro experiments, we demonstrated that serum amylase values obtained in the samples diluted in a 1:1 ratio with HES 130/0.4 and in samples diluted in a 1:1 ratio with 0.9% NaCl displayed a negligible median difference of 3%.
  
  
  CONCLUSIONS
  The in vivo effect of HES 130/0.4 administration on serum amylase activity observed in our study was pharmacological (real) in nature. With the exception of the influence of HES 130/0.4 on amylase activity, the effects of HES 130/0.4 on other parameters tested in this study can be interpreted as having no clinical relevance.
authors.affiliation: Department of Laboratory Medicine, Konventhospital Barmherzige Brueder, Linz, Austria. thomas.mueller@bs-lab.at
authors.forename: Thomas
authors.initials: T
authors.lastname: Mueller
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Schimetta
authors.forename: Benjamin
authors.initials: B
authors.lastname: Dieplinger
authors.forename: Peter
authors.initials: P
authors.lastname: Loeffler
authors.forename: Markus
authors.initials: M
authors.lastname: Rehm
authors.forename: Uwe
authors.initials: U
authors.lastname: Kreimeier
authors.forename: Werner
authors.initials: W
authors.lastname: Poelz
authors.forename: Meinhard
authors.initials: M
authors.lastname: Haltmayer
mesh_terms: D000328:Adult; D001774:Blood Chemical Analysis / Q000379:methods* / Q000592:standards*; D002624:Chemistry, Clinical / Q000379:methods* / Q000592:standards; D004912:Erythrocytes / Q000166:cytology; D005260:Female; D006403:Hematologic Tests; D006405:Hematology / Q000379:methods / Q000592:standards; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D006895:Hydroxyethyl Starch Derivatives / Q000627:therapeutic use*; D008297:Male; D008875:Middle Aged; D010952:Plasma Substitutes / Q000737:chemistry / Q000627:therapeutic use*; D015203:Reproducibility of Results
chemical_list: D006454:Hemoglobins; D006895:Hydroxyethyl Starch Derivatives; D010952:Plasma Substitutes