Testis specific lactate dehydrogenase as target for immunoliposomes.
OBJECTIVE
Is it possible to deliver therapeutic agents to testis through specific targeting?


METHODS
Immunoliposomes are designed by incorporating antibodies to lactate dehydrogenase-C4 (LDH-C(4)), which is the product of a testis specific gene. Their targeting and delivering ability is investigated in vitro and in vivo.


RESULTS
It is observed that LDHC(4)-immunoliposomes are able to discriminate and recognize antigens on spermatozoa and testes both in vitro and in vivo.


CONCLUSIONS
Specific targeting through LDH-C(4) appears to be a feasible strategy for delivering therapeutic as well as anti-spermatogenic agents to testis.
Dutta
Ranjna C
RC
Department of Biochemistry, Molecular Biology and Cell Biology Northwestern University, Evanston, IL 60208, USA. ranjna_dutta@rediffmail.com
Goldberg
Erwin
E
D000818:Animals; D000906:Antibodies / Q000008:administration & dosage*; D000918:Antibody Specificity; D015153:Blotting, Western; D016503:Drug Delivery Systems / Q000379:methods*; D004797:Enzyme-Linked Immunosorbent Assay; D007527:Isoenzymes / Q000008:administration & dosage / Q000276:immunology / Q000493:pharmacokinetics; D007770:L-Lactate Dehydrogenase / Q000008:administration & dosage* / Q000276:immunology / Q000493:pharmacokinetics; D008081:Liposomes / Q000008:administration & dosage* / Q000276:immunology / Q000493:pharmacokinetics; D008297:Male; D051379:Mice; D013094:Spermatozoa / Q000276:immunology; D013737:Testis / Q000276:immunology*; D014018:Tissue Distribution
D000906:Antibodies; D007527:Isoenzymes; D008081:Liposomes; D007770:L-Lactate Dehydrogenase; C409673:lactate dehydrogenase C4