The LDL receptor-related protein can form homo-dimers in neuronal cells.
The ability of the low density lipoprotein receptor-related protein (LRP) to form homo-dimers was studied in mouse neuroblastoma and human neuroglioma cells as well as in primary cortical cultures from adult mouse brain. Homo-dimerization of LRP light chain (LC) was shown by several methods including co-immunoprecipitation, fluorescence lifetime imaging microscopy, and bimolecular fluorescence complementation assay. The requirement of intact NPXY motifs of LRP LC for homo-dimerization was ruled out by co-immunoprecipitation assay.
Makarova
Alexandra
A
MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
Bercury
Kathryn K
KK
Adams
Kenneth W
KW
Joyner
Daniel
D
Deng
Meihua
M
Spoelgen
Robert
R
Koker
Mirjam
M
Strickland
Dudley K
DK
Hyman
Bradley T
BT
D020816:Amino Acid Motifs; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004622:Embryo, Mammalian; D004705:Endocytosis; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017386:Sequence Homology, Amino Acid; D014162:Transfection / Q000379:methods
D026503:Low Density Lipoprotein Receptor-Related Protein-1; D049452:Green Fluorescent Proteins