title: Cleavage of the signaling mucin Msb2 by the aspartyl protease Yps1 is required for MAPK activation in yeast.
abstract: Signaling mucins are cell adhesion molecules that activate RAS/RHO guanosine triphosphatases and their effector mitogen-activated protein kinase (MAPK) pathways. We found that the Saccharomyces cerevisiae mucin Msb2p, which functions at the head of the Cdc42p-dependent MAPK pathway that controls filamentous growth, is processed into secreted and cell-associated forms. Cleavage of the extracellular inhibitory domain of Msb2p by the aspartyl protease Yps1p generated the active form of the protein by a mechanism incorporating cellular nutritional status. Activated Msb2p functioned through the tetraspan protein Sho1p to induce MAPK activation as well as cell polarization, which involved the Cdc42p guanine nucleotide exchange factor Cdc24p. We postulate that cleavage-dependent activation is a general feature of signaling mucins, which brings to light a novel regulatory aspect of this class of signaling adhesion molecule.
authors.affiliation: Department of Biological Sciences, State University of New York at Buffalo, Buffalo, NY 14260, USA.
authors.forename: Nadia
authors.initials: N
authors.lastname: Vadaie
authors.forename: Heather
authors.initials: H
authors.lastname: Dionne
authors.forename: Darowan S
authors.initials: DS
authors.lastname: Akajagbor
authors.forename: Seth R
authors.initials: SR
authors.lastname: Nickerson
authors.forename: Damian J
authors.initials: DJ
authors.lastname: Krysan
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Cullen
mesh_terms: D016282:Aspartic Acid Endopeptidases / Q000096:biosynthesis / Q000378:metabolism*; D004789:Enzyme Activation; D004790:Enzyme Induction; D020690:GTPase-Activating Proteins / Q000737:chemistry / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism; D047908:Intracellular Signaling Peptides and Proteins; D020935:MAP Kinase Signaling System; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism*; D009077:Mucins / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D011499:Protein Processing, Post-Translational; D017434:Protein Structure, Tertiary; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D020690:GTPase-Activating Proteins; D020662:Guanine Nucleotide Exchange Factors; D047908:Intracellular Signaling Peptides and Proteins; C076360:MSB2 protein, S cerevisiae; D009077:Mucins; D010455:Peptides; D029701:Saccharomyces cerevisiae Proteins; D020928:Mitogen-Activated Protein Kinases; D016282:Aspartic Acid Endopeptidases; C499266:YPS1 protein, S cerevisiae