title: Gibberellin-regulated gene in the basal region of rice leaf sheath encodes basic helix-loop-helix transcription factor.
abstract: Genes regulated by gibberellin (GA) during leaf sheath elongation in rice seedlings were identified using the transcriptome approach. mRNA from the basal regions of leaf sheaths treated with GA3 was analyzed by high-coverage gene expression profiling. 33,004 peaks were detected, and 30 transcripts showed significant changes in the presence of GA3. Among these, basic helix-loop-helix transcription factor (AK073385) was significantly upregulated. Quantitative PCR analysis confirmed that expression of AK073385 was controlled by GA3 in a time- and dose-dependent manner. Basic helix-loop-helix transcription factor (AK073385) is therefore involved in the regulation of gene expression by GA3.
authors.affiliation: National Institute of Crop Science, Kannondai 2-1-18, Tsukuba 305-8518, Japan. skomatsu@affrc.go.jp
authors.forename: Setsuko
authors.initials: S
authors.lastname: Komatsu
authors.forename: Hironori
authors.initials: H
authors.lastname: Takasaki
mesh_terms: D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D005875:Gibberellins / Q000378:metabolism*; D007501:Iron / Q000378:metabolism; D046228:Microarray Analysis; D008969:Molecular Sequence Data; D012275:Oryza / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D018515:Plant Leaves / Q000033:anatomy & histology / Q000235:genetics / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D005875:Gibberellins; D007501:Iron; D010937:Plant Growth Regulators; D010940:Plant Proteins