title: Footprinting analysis demonstrates extensive similarity between eukaryotic RNase P and RNase MRP holoenzymes.
abstract: Eukaryotic ribonuclease (RNase) P and RNase MRP are evolutionary related RNA-based enzymes involved in metabolism of various RNA molecules, including tRNA and rRNA. In contrast to the closely related eubacterial RNase P, which is comprised of an RNA component and a single small protein, these enzymes contain multiple protein components. Here we report the results of footprinting studies performed on purified Saccharomyces cerevisiae RNase MRP and RNase P holoenzymes. The results identify regions of the RNA components affected by the protein moiety, suggest a role of the proteins in stabilization of the RNA fold, and point to substantial similarities between the two evolutionary related RNA-based enzymes.
authors.affiliation: Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.
authors.forename: Olga
authors.initials: O
authors.lastname: Esakova
authors.forename: Anna
authors.initials: A
authors.lastname: Perederina
authors.forename: Chao
authors.initials: C
authors.lastname: Quan
authors.forename: Mark E
authors.initials: ME
authors.lastname: Schmitt
authors.forename: Andrey S
authors.initials: AS
authors.lastname: Krasilnikov
mesh_terms: D001483:Base Sequence; D004722:Endoribonucleases / Q000737:chemistry* / Q000378:metabolism; D019143:Evolution, Molecular; D008969:Molecular Sequence Data; D023302:Protein Footprinting; D016337:RNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D012331:RNA, Fungal / Q000737:chemistry / Q000378:metabolism*; D043262:Ribonuclease P / Q000737:chemistry* / Q000378:metabolism; D012261:Ribonucleoproteins / Q000737:chemistry* / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D016337:RNA, Catalytic; D012331:RNA, Fungal; D012261:Ribonucleoproteins; D029701:Saccharomyces cerevisiae Proteins; D004722:Endoribonucleases; C058330:mitochondrial RNA-processing endoribonuclease; D043262:Ribonuclease P