title: [RT-PCR-based methods for identification and typing of infectious hemopoietic necrosis virus in salmons].
abstract: A RT-PCR method has been developed to diagnose infectious hemopoietic necrosis virus (IHNV) in salmons. The authors show it possible to use the method for viral shedding in both a cell culture and a clinical sample from infected fishes. Genotyping of IHNV strains originating from North America, Europe, and Russia, by using the restriction fragment length polymerase analysis, has revealed that 10 of them belong to 3 existing genogroups (U, M, and L). Three Russian isolates are assigned into a separate subgroup. Phylogenetic analysis of several isolates has confirmed that viral strains from Katchatka belong to the North American U-genogroup whereas 3 Russian isolates from the continental zone of the country make up a separate subgroup within the same genogroup.
authors.forename: A G
authors.initials: AG
authors.lastname: Popova
authors.forename: S F
authors.initials: SF
authors.lastname: Oreshkova
authors.forename: I S
authors.initials: IS
authors.lastname: Zhchelkunov
authors.forename: S L
authors.initials: SL
authors.lastname: Rudakova
authors.forename: T I
authors.initials: TI
authors.lastname: Zhchelkunova
authors.forename: N V
authors.initials: NV
authors.lastname: Tikunova
authors.forename: N N
authors.initials: NN
authors.lastname: Blinova
authors.forename: A A
authors.initials: AA
authors.lastname: Il'ichev
mesh_terms: D000818:Animals; D017931:DNA Primers; D005393:Fish Diseases / Q000175:diagnosis* / Q000821:virology; D005814:Genes, Viral; D029142:Infectious hematopoietic necrosis virus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D018353:Rhabdoviridae Infections / Q000175:diagnosis / Q000662:veterinary*; D012474:Salmon / Q000821:virology*; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers