Lactobacillus senmaizukei sp. nov., isolated from Japanese pickle.
The taxonomic position of a lactic acid bacterium, strain L13(T), isolated from senmaizuke, a Japanese traditional pickle, was studied. This strain was a heterofermentative, facultatively anaerobic, Gram-positive, non-spore-forming, non-motile, rod-shaped bacterium. It produced dl-lactic acid from glucose. Phylogenetic analysis of its 16S rRNA gene sequence and physiological and biochemical characteristics indicated that the strain was a member of the genus Lactobacillus. Based on 16S rRNA gene sequence similarity, strain L13(T) was closely related to Lactobacillus hammesii CIP 108387(T) (98.9 %) and Lactobacillus parabrevis ATCC 53295(T) (98.5 %). In addition, the rpoA gene sequence of strain L13(T) was closely related to those of L. hammesii CIP 108387(T) (92.2 %) and L. parabrevis ATCC 53295(T) (92.0 %). However, DNA-DNA hybridization of strain L13(T) with these two strains revealed that strain L13(T) represents a separate genomic species. The DNA G+C content of strain L13(T) was 46 mol% and the peptidoglycan was of the A4alpha l-Lys-d-Asp type. Thus, these data indicate that strain L13(T) represents a novel species of the genus Lactobacillus, for which the name Lactobacillus senmaizukei is proposed. The type strain is L13(T) (=NBRC 103853(T)=TISTR 1847(T)).
Hiraga
Kazumi
K
Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Goshokaido-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan.
Ueno
Yoshie
Y
Sukontasing
Sirapan
S
Tanasupawat
Somboon
S
Oda
Kohei
K
D005516:Food Microbiology; D007564:Japan; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D012336:RNA, Ribosomal, 16S