The hydrolysis of triglycerides by immobilized lipase in a hydrophilic membrane reactor.
In the present article a method is described to immobilize lipase from Candida rugosa on a hollow fiber membrane, and the use of such a system for the hydrolysis of lipids is reported. The membranes were ENKA hydrophilic Cuprophan-type hollow fibers, having a large specific surface area. The immobilized lipase exhibited a high stability: the half-life time was 43 days at a temperature of 30 degrees C. Furthermore, it is proved that kinetic studies can be carried out with this system, operated in a batch or continuous mode. The relation between conversion rate and degree of hydrolysis was determined. On this basis, a dynamic model of the process was developed that describes the relation between reaction conditions and the conversion rate.
Pronk
W
W
Department of Food Science, Food and Bioengineering Group, Agricultural University Wageningen, De Dreijen 12, 6703 BC Wageningen, The Netherlands.
Kerkhof
P J
PJ
Van Helden
C
C
Riet
K V
KV