title: A laminar flow model of aerosol survival of epidemic and non-epidemic strains of Pseudomonas aeruginosa isolated from people with cystic fibrosis.
abstract: BACKGROUND
  Cystic fibrosis (CF) is an inherited multi-system disorder characterised by chronic airway infection with pathogens such as Pseudomonas aeruginosa. Acquisition of P. aeruginosa by patients with CF is usually from the environment, but recent studies have demonstrated patient to patient transmission of certain epidemic strains, possibly via an airborne route. This study was designed to examine the survival of P. aeruginosa within artificially generated aerosols.
  
  
  RESULTS
  Survival was effected by the solution used for aerosol generation. Within the aerosols it was adversely affected by an increase in air temperature. Both epidemic and non-epidemic strains of P. aeruginosa were able to survive within the aerosols, but strains expressing a mucoid phenotype had a survival advantage.
  
  
  CONCLUSIONS
  This would suggest that segregating individuals free of P. aeruginosa from those with chronic P. aeruginosa infection who are more likely to be infected with mucoid strains may help reduce the risk of cross-infection. Environmental factors also appear to influence bacterial survival. Warming and drying the air within clinical areas and avoidance of humidification devices may also be beneficial in reducing the risk of cross-infection.
authors.affiliation: Regional Cystic Fibrosis Unit, St James University Hospital, Leeds, UK. ian.clifton@leedsth.nhs.uk
authors.forename: Ian J
authors.initials: IJ
authors.lastname: Clifton
authors.forename: Louise A
authors.initials: LA
authors.lastname: Fletcher
authors.forename: Clive B
authors.initials: CB
authors.lastname: Beggs
authors.forename: Miles
authors.initials: M
authors.lastname: Denton
authors.forename: Daniel G
authors.initials: DG
authors.lastname: Peckham
mesh_terms: D000336:Aerosols / Q000009:adverse effects / Q000737:chemistry*; D000391:Air Microbiology; D003550:Cystic Fibrosis / Q000382:microbiology*; D006801:Humans; D007552:Isotonic Solutions / Q000494:pharmacology; D050296:Microbial Viability; D010316:Particle Size; D011552:Pseudomonas Infections / Q000453:epidemiology / Q000382:microbiology / Q000635:transmission*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000254:growth & development* / Q000302:isolation & purification; D000077331:Ringer's Solution; D014867:Water / Q000494:pharmacology
chemical_list: D000336:Aerosols; D007552:Isotonic Solutions; D014867:Water; D000077331:Ringer's Solution