title: [High-performance liquid chromatography-mass spectrometry for determining olmesartan in human plasma].
abstract: OBJECTIVE
  To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma.
  
  
  METHODS
  Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(14:60:26) with a flow rate of 0.22 ml/min.
  
  
  RESULTS
  The lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%.
  
  
  CONCLUSIONS
  The method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.
authors.affiliation: Department of Pharmacy, Liling Hospital of Traditional Chinese Medicine, Liling 420000, China.
authors.forename: Shi-Heng
authors.initials: SH
authors.lastname: Chen
authors.forename: Cui-Fang
authors.initials: CF
authors.lastname: Wu
authors.forename: Ben-Mei
authors.initials: BM
authors.lastname: Chen
authors.forename: Qi
authors.initials: Q
authors.lastname: Pei
authors.forename: Hong-Yi
authors.initials: HY
authors.lastname: Tan
authors.forename: Li
authors.initials: L
authors.lastname: Yang
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Yang
mesh_terms: D047228:Angiotensin II Type 1 Receptor Blockers / Q000097:blood; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007093:Imidazoles / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods; D015203:Reproducibility of Results; D013777:Tetrazoles / Q000097:blood*
chemical_list: D047228:Angiotensin II Type 1 Receptor Blockers; D007093:Imidazoles; D013777:Tetrazoles; C437965:olmesartan