Expression of matrix metalloproteinases 2 and 9 in odontogenic myxoma in vivo and in vitro.
Odontogenic myxoma is a benign neoplasm, which presents local invasiveness and tendency for recurrence. Matrix metalloproteinases (MMPs) 2 and 9 are involved in tumor invasion. Thus, the aim of this study was to analyze the expression and activity of these MMPs in odontogenic myxoma in vivo and in vitro. Three cases of odontogenic myxoma and cultured cells derived from this tumor (Mix1 cell line) were used. The detection and activity of two MMPs (2 and 9) were performed by immunohistochemistry in formalin-fixed, paraffin-embedded sections of odontogenic myxoma and immunofluorescence of the cultured cells and, by gelatin zymographic analysis of Mix1 conditioned media, respectively. MMPs 2 and 9 were detected in vivo and in vitro. The zymographic assay detected latent MMP-2 as well as latent and active MMP-9. Based on our findings, we suggest that MMPs may be involved in local invasiveness of the odontogenic myxoma. MMP-9 is not only secreted by odontogenic myxoma but also has enzyme activity with no further stimulation. Other MMPs were not analyzed; however, our results suggest that the invasive behaviour of odontogenic myxoma could be related at least to MMP-9.
Miyagi
Sueli P H
SP
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Hiraki
Karen R N
KR
Martins
Manoela D
MD
Marques
Márcia M
MM
D000293:Adolescent; D000328:Adult; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000201:enzymology; D017077:Culture Media, Conditioned; D003593:Cytoplasm / Q000201:enzymology; D005109:Extracellular Matrix / Q000201:enzymology; D005260:Female; D019085:Fluorescent Antibody Technique, Direct; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D020778:Matrix Metalloproteinase 2 / Q000032:analysis*; D020780:Matrix Metalloproteinase 9 / Q000032:analysis*; D009361:Neoplasm Invasiveness; D009808:Odontogenic Tumors / Q000201:enzymology*
D017077:Culture Media, Conditioned; C522361:MMP2 protein, human; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9