title: Leptin G-2548A promoter polymorphism is associated with increased plasma leptin and BMI in Brazilian women.
abstract: Variants in leptin gene (LEP) have been implicated in the pathogenesis of obesity. The relationship between LEP G-2548A polymorphism and obesity-related traits was evaluated in a sample of Brazilian women (n = 228) who were randomly selected from two clinical centers in Sao Paulo city. Blood samples were collected for DNA extraction, plasma leptin and serum lipids measurements. LEP G-2548A genotypes were identified by a PCR- RFLP strategy using the endonuclease Alw44I. LEP G-2548A was associated with obesity after adjustment for covariates (age, hypertension, coronary artery disease, smoking and physical activity). Women carrying G allele had a four times higher risk of obesity than the A allele carriers (OR: 4.11, CI95%: 1.06-15.90, p = 0.041). G allele was also related to increased plasma leptin (p = 0.024) and body mass index (p = 0.027). Hypertension, hyperglycemia, dyslipidemia and coronary artery disease were associated with obesity. However LEP G-2548A polymorphism was not related to these variables. All together these data suggest that LEP G-2548A polymorphism has an important role in regulating plasma leptin levels and body mass index in women.
authors.affiliation: Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, SP, Brasil.
authors.forename: Hamilton M
authors.initials: HM
authors.lastname: Hinuy
authors.forename: Mario H
authors.initials: MH
authors.lastname: Hirata
authors.forename: Neusa
authors.initials: N
authors.lastname: Forti
authors.forename: Jayme
authors.initials: J
authors.lastname: Diament
authors.forename: Marcelo F
authors.initials: MF
authors.lastname: Sampaio
authors.forename: Dikran
authors.initials: D
authors.lastname: Armaganijan
authors.forename: Luis A
authors.initials: LA
authors.lastname: Salazar
authors.forename: Rosario D C
authors.initials: RD
authors.lastname: Hirata
mesh_terms: D015992:Body Mass Index; D001938:Brazil; D004247:DNA / Q000032:analysis; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D020738:Leptin / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D009765:Obesity / Q000097:blood* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic / Q000235:genetics; D012150:Polymorphism, Restriction Fragment Length; D011401:Promoter Regions, Genetic / Q000235:genetics*
chemical_list: D020738:Leptin; D004247:DNA