title: [Effect of METH1 gene transfection on the proliferation of rabbit's ear scar].
abstract: OBJECTIVE
  To investigate the effect of METH1 gene transfection on fibroblast proliferation and I, III collagen synthesis in rabbit ear scar.
  
  
  METHODS
  The hypertrophic scar model on the rabbit ears was reproduced. 10 days after epithelization, Ad-METH1 was injected into the scar tissue. 30 days later, the effect of METH1 gene transfection on the angiogenesis, fibroblast proliferation and the ratio of collagen I/III in the scar tissue was detected by microcirculation microscope, AgNOR particle count and collagen dyeing.
  
  
  RESULTS
  30 days after injection of Ad-METH1, angiogenesis, fibroblast proliferation and the ratio of collagen I/III in the scar tissue were obviously suppressed.
  
  
  CONCLUSIONS
  Early application of Ad-METH1 after epithelization can markedly inhibit the formation of the hypertrophic scar.
authors.affiliation: Department of Plastic Surgery, Xijing Hospital, the Fourth Military Medical University. Xi'an 710032, China.
authors.forename: Bao-Qiang
authors.initials: BQ
authors.lastname: Song
authors.forename: Kai-Hua
authors.initials: KH
authors.lastname: Lu
authors.forename: Shu-Zhong
authors.initials: SZ
authors.lastname: Guo
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.forename: Pai
authors.initials: P
authors.lastname: Peng
authors.forename: Fu-Cheng
authors.initials: FC
authors.lastname: Ma
authors.forename: Hui-Yuan
authors.initials: HY
authors.lastname: Li
mesh_terms: D051722:ADAM Proteins / Q000235:genetics*; D020533:Angiogenesis Inhibitors / Q000235:genetics*; D000818:Animals; D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000473:pathology*; D004195:Disease Models, Animal; D004423:Ear / Q000473:pathology; D004431:Ear, External / Q000473:pathology; D005260:Female; D008297:Male; D008833:Microcirculation; D009389:Neovascularization, Pathologic; D011817:Rabbits; D014162:Transfection; D014945:Wound Healing
chemical_list: D020533:Angiogenesis Inhibitors; D051722:ADAM Proteins