Trb2, a mouse homolog of tribbles, is dispensable for kidney and mouse development.
Glomeruli comprise an important filtering apparatus in the kidney and are derived from the metanephric mesenchyme. A nuclear protein, Sall1, is expressed in this mesenchyme, and we previously reported that Trb2, a mouse homolog of Drosophila tribbles, is expressed in the mesenchyme-derived tissues of the kidney by microarray analyses using Sall1-GFP knock-in mice. In the present report, we detected Trb2 expression in a variety of organs during gestation, including the kidneys, mesonephros, testes, heart, eyes, thymus, blood vessels, muscle, bones, tongue, spinal cord, and ganglions. In the developing kidney, Trb2 signals were detected in podocytes and the prospective mesangium of the glomeruli, as well as in ureteric bud tips. However, Trb2 mutant mice did not display any apparent phenotypes and no proteinuria was observed, indicating normal glomerular functions. These results suggest that Trb2 plays minimal roles during kidney and mouse development.
Takasato
Minoru
M
Division of Integrative Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
Kobayashi
Chiyoko
C
Okabayashi
Koji
K
Kiyonari
Hiroshi
H
Oshima
Naoko
N
Asashima
Makoto
M
Nishinakamura
Ryuichi
R
D000818:Animals; D047108:Embryonic Development / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000235:genetics; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D007678:Kidney Glomerulus / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D020411:Oligonucleotide Array Sequence Analysis; D010641:Phenotype; D050199:Podocytes / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism / Q000502:physiology* / Q000037:antagonists & inhibitors; D014018:Tissue Distribution; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014513:Ureter / Q000196:embryology / Q000378:metabolism
D047908:Intracellular Signaling Peptides and Proteins; C491254:Sall1 protein, mouse; D014157:Transcription Factors; C515847:tribbles 2 protein, mouse; D049452:Green Fluorescent Proteins; D017346:Protein Serine-Threonine Kinases