Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts.
The effects of laser phototherapy on the release of growth factors by human gingival fibroblasts were studied in vitro. Cells from a primary culture were irradiated twice (6 h interval), with continuous diode laser [gallium-aluminum-arsenium (GaAlAs), 780 nm, or indium-gallium-aluminum-phosphide (InGaAlP),_660 nm] in punctual and contact mode, 40 mW, spot size 0.042 cm(2), 3 J/cm(2) and 5 J/cm(2) (3 s and 5 s, respectively). Positive [10% fetal bovine serum (FBS)] and negative (1%FBS) controls were not irradiated. Production of keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) was quantified by enzyme-linked immunosorbent assay (ELISA). The data were statistically compared by analysis of variance (ANOVA) followed by Tukey's test (P </= 0.05). The characterization of the cell line indicated a mesenchymal nature. KGF release was similar in all groups, while that of bFGF was significantly greater (1.49-times) in groups treated with infra-red laser. It was concluded that increased production of bFGF could be one of the mechanisms by which infra-red laser stimulates wound healing.
Damante
Carla Andreotti
CA
Departamento de Periodontia, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, Brazil.
De Micheli
Giorgio
G
Miyagi
Sueli Patrícia Harumi
SP
Feist
Ilíria Salomão
IS
Marques
Márcia Martins
MM
D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D051523:Fibroblast Growth Factor 7 / Q000096:biosynthesis; D005346:Fibroblast Growth Factors / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D005881:Gingiva / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D005893:Gingivoplasty; D006801:Humans; D028022:Low-Level Light Therapy; D010789:Phototherapy; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects
D003470:Culture Media; C496453:FGF7 protein, human; D016222:Fibroblast Growth Factor 2; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors