title: Anaplasma marginale major surface protein 1a directs cell surface display of tick BM95 immunogenic peptides on Escherichia coli.
abstract: The surface display of heterologous proteins on live Escherichia coli using anchoring motifs from outer membranes proteins has impacted on many areas of biochemistry, molecular biology and biotechnology. The Anaplasma marginale major surface protein 1a (MSP1a) contains N-terminal surface-exposed repeated peptides (28-289 amino acids) that are involved in pathogen interaction with host cell receptors and is surface-displayed when the recombinant protein is expressed in E. coli. Therefore, it was predicted that MSP1a would surface display on E. coli peptides inserted in the N-terminal repeats region of the protein. The Rhipicephalus (Boophilus) microplus BM86 and BM95 glycoproteins are homologous proteins that protect cattle against tick infestations. In this study, we demonstrated that a recombinant protein comprising tick BM95 immunogenic peptides fused to the A. marginale MSP1a N-terminal region is displayed on the E. coli surface and is recognized by anti-BM86 and anti-MSP1a antibodies. This system provides a novel approach to the surface display of heterologous antigenic proteins on live E. coli and suggests the possibility to use the recombinant bacteria for immunization studies against cattle tick infestations.
authors.affiliation: Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain.
authors.forename: Mario
authors.initials: M
authors.lastname: Canales
authors.forename: Consuelo
authors.initials: C
authors.lastname: Almazán
authors.forename: José M
authors.initials: JM
authors.lastname: Pérez de la Lastra
authors.forename: José
authors.initials: J
authors.lastname: de la Fuente
mesh_terms: D042322:Anaplasma marginale / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism*; D004926:Escherichia coli / Q000276:immunology / Q000378:metabolism*; D005822:Genetic Vectors; D007114:Immunization; D007700:Kinetics; D010455:Peptides / Q000276:immunology*; D021381:Protein Transport; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013987:Ticks / Q000276:immunology*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D010455:Peptides; D011993:Recombinant Fusion Proteins; C449089:major surface protein 1a, Anaplasma