title: Improved degradation of organophosphate dichlorvos by Trichoderma atroviride transformants generated by restriction enzyme-mediated integration (REMI).
abstract: A simple technique, REMI (restriction enzyme-mediated integration), was used to construct transformants of Trichoderma atroviride with improved capability of degrading organophosphate pesticide dichlorvos. Linearized DNA of plasmid pV2 bearing the hygromycin B phosphotransferase (hph) gene was inserted into chromosomes of wild strain T23 and transformation was confirmed by PCR and Southern blot analysis, respectively. Of 247 transformants, 76% showed improved dichlorvos degradation ability as compared to the parent strain T23 based on the least significant difference (LSD) test at p=0.01. Among them, 8 transformants exhibited 30% higher in degradation rate than the parent isolate. The highest dichlorvos degradation rate of the transformants was up to 96%. This study provided an effective approach for improving organophosphate pesticide-degrading capability of T. atroviride.
authors.affiliation: Department of Plant Science, School of Agriculture and Biology, Key laboratory of Microorganism Metabolism, Ministry of Education, Shanghai Jiaotong University, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Tang
authors.forename: Lixing
authors.initials: L
authors.lastname: Liu
authors.forename: Shifeng
authors.initials: S
authors.lastname: Hu
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Chen
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D001673:Biodegradation, Environmental; D004006:Dichlorvos / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004006:Dichlorvos; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase