title: Synthesis of a novel pH-sensitive methacrylate amphiphilic polymer and its primary application in aqueous two-phase systems.
abstract: In this study, a novel pH-sensitive and reversible water-soluble polymer(P(ABC)) forming aqueous two-phase systems(ATPS) was synthesized by using 2-(dimethylamino)ethyl methacrylate, t-butyl methacrylate, and methyl methacrylate as monomers and 2,2'-azo-bis-isobutyronitrile as initiator. The P(ABC) could be recovered by adjusting isoelectric point (PI) to 8.4, and recovery at PI could reach 95%. ATPS was formed by 5% (w/w) P(ABC) and 10% (w/w) PEG20000. The partition coefficient K of lysozyme was 6.8, and the partition coefficient K of bovine serum albumin could reach 12.5 in the ATPS.
authors.affiliation: State Key laboratory of Bioreactor Engineering, Department of Biochemical Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qin
authors.forename: Xue-Jun
authors.initials: XJ
authors.lastname: Cao
mesh_terms: D006863:Hydrogen-Ion Concentration; D008689:Methacrylates / Q000138:chemical synthesis* / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D014867:Water / Q000737:chemistry*
chemical_list: D008689:Methacrylates; D011108:Polymers; D014867:Water; D009113:Muramidase