title: [Determination of nitrofuran metabolites residues in animal tissues by LC-MS/MS method].
abstract: A LC-MS-MS method is presented to analyze simultaneously the metabolites of four nitrofuran veterinary drugs in animal muscle tissue e.g., furazolidone, furaltadone, nitofurantoina and nitrofurazone. The sample clean up were performed by a liquid-liquid extraction with ethyl acetate, after a hydrolysis and derivatization with 2-nitrobenzaldehyde. Nitrofurane metabolites were determined by LC-ESI-MS/MS in positive mode. The LC was equipped with column Luna C18 Phenomenex. A binary gradient mobile phase was used as methanol solvent B containing 0.5 mM ammonium acetate and methanol (80:20 v/v). The method was validated according to criteria of Decision Commission No 2002/657/EC. Samples were fortified with metabolites of nitrofuran between 0.5-2.0 microg/kg with AOZ-d4, and AMOZ-d5 as internal standard. The mean recoveries from meat spiked at 1.0 microg/kg were 84.5-109.7%. Limit of decision (CCalpha) was between 0.25-0.57 and capability of detection (CCbeta) 0.32-0.77 microg/kg.
authors.affiliation: Pracownia Badań Chemicznych Srodków Spozywczych Zakład Higieny Weterynaryjnej 15-959 Białystok, ul. Zwycigstwa 26a. rodziewicz@wiw.bianet.com.pl
authors.forename: Lech
authors.initials: L
authors.lastname: Rodziewicz
authors.forename: Iwona
authors.initials: I
authors.lastname: Zawadzka
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D008892:Milk / Q000737:chemistry*; D009581:Nitrofurans / Q000032:analysis*; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D009581:Nitrofurans; D019155:Veterinary Drugs