title: Purification and partial characterization of coxsackievirus B3 2A protease expressed in Escherichia coli.
abstract: Reported here is the overexpression, purification and partial characterization of recombinant coxsakievirus B3 2A protease (CVB3 2Apro) from bacterial cells transformed with a plasmid containing the CVB3 2Apro cDNA sequences. The structural investigation showed that the protein contains mostly beta-strand elements and requires Zn2+ ions as a structural component which appeared to be inhibitory if added exogenously. The purified enzyme activity was optimal at 4 degrees C and had a short half-life at physiological temperature. This feature can be the result of the presence of a high content of beta-structure and also hydrophobic residues in its structure.
authors.affiliation: Neuroscience Research Center, Shahid Beheshti University, M.C., Tehran, Iran. nmaghsoudi@sbmu.ac.ir
authors.forename: Nader
authors.initials: N
authors.lastname: Maghsoudi
authors.forename: Fariba
authors.initials: F
authors.lastname: Khodagholi
authors.forename: Mahnaz
authors.initials: M
authors.lastname: Sadjadi
authors.forename: Mehdi
authors.initials: M
authors.lastname: Zeinodini
authors.forename: Marjan
authors.initials: M
authors.lastname: Sabbaghian
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002942:Circular Dichroism; D003001:Cloning, Molecular; D003546:Cysteine Endopeptidases / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D004353:Drug Evaluation, Preclinical; D029822:Enterovirus B, Human / Q000201:enzymology* / Q000235:genetics; D004791:Enzyme Inhibitors / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics*; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D011489:Protein Denaturation; D013050:Spectrometry, Fluorescence; D013696:Temperature; D014466:Ultraviolet Rays; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology
chemical_list: D004791:Enzyme Inhibitors; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C057400:picornain 2A, Picornavirus; D015032:Zinc