title: Establishment of a feline astrocyte-derived cell line (G355-5 cells) expressing feline CD134 and a rapid quantitative assay for T-lymphotropic feline immunodeficiency viruses.
abstract: Few laboratory strains of feline immunodeficiency virus (FIV) can infect Crandell feline kidney cells (an epithelial-type of cells), however, most primary isolates are T-lymphotropic. T-lymphotropic FIV requires both feline CD134 (an activation marker of helper T-lymphocytes) and CXCR4 (a chemokine receptor) in infection as primary and secondary receptors, respectively. Using feline T-lymphoblastoid cell lines, titration of primary FIV isolates was carried out, however the titration assay was laborious and time-consuming. In this study, using G355-5 cells (a feline astrocyte-derived cell line) transduced with a cDNA of feline CD134 as target cells, an assay system was developed to quantitate primary FIV isolates. With a previous method using a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive to FIV, it took 12 days to complete the assay, however, it took only 2 days with the new method. The FIV-infected cells became in a state of persistent infection, producing a large amount of FIV, indicating that the cells will be useful for propagation of T-lymphotropic FIV strains.
authors.affiliation: Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan; Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-Nihonmatsucho, Sakyo-ku, Kyoto 606-8501, Japan.
authors.forename: Mieko
authors.initials: M
authors.lastname: Ishikawa
authors.affiliation: Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Masaya
authors.initials: M
authors.lastname: Okada
authors.affiliation: Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Baba
authors.affiliation: Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Shojima
authors.affiliation: Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo 108-8639, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Shimojima
authors.affiliation: Graduate School of Human and Environmental Studies, Kyoto University, Yoshida-Nihonmatsucho, Sakyo-ku, Kyoto 606-8501, Japan; Laboratory of Primate Model, Experimental Research Center for Infectious Diseases, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Miura
authors.affiliation: Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Miyazawa
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000276:immunology* / Q000821:virology*; D002415:Cats; D002460:Cell Line; D005434:Flow Cytometry; D016089:Immunodeficiency Virus, Feline / Q000254:growth & development* / Q000302:isolation & purification; D053262:Receptors, OX40 / Q000032:analysis*
chemical_list: D053262:Receptors, OX40