title: Neuropeptide Y (NPY) Y2 receptors of rabbit kidney cortex are largely dimeric.
abstract: The neuropeptide Y (NPY) Y2 receptors and the pancreatic polypeptide Y4 receptors from rabbit kidney cortex are isolated largely as approximately 180 kDa complexes constituted of one receptor dimer and one G-protein heterotrimer, similar to NPY receptors expressed in the Chinese hamster ovary (CHO) cells. As expected, kidney and CHO cell Y2 dimers are converted into monomers by increasing concentrations of a selective agonist. Prevalence of dimeric Y2 receptors in the kidney could be related to low plasma levels of Y2 agonists, and possibly also to a relatively low concentration of Gi alpha subunits.
authors.affiliation: Department of Pharmacology, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
authors.forename: A M
authors.initials: AM
authors.lastname: Estes
authors.forename: Y Y
authors.initials: YY
authors.lastname: Wong
authors.forename: M S
authors.initials: MS
authors.lastname: Parker
authors.forename: F R
authors.initials: FR
authors.lastname: Sallee
authors.forename: A
authors.initials: A
authors.lastname: Balasubramaniam
authors.forename: S L
authors.initials: SL
authors.lastname: Parker
mesh_terms: D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D019281:Dimerization; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D011817:Rabbits; D017476:Receptors, Neuropeptide Y / Q000819:agonists / Q000378:metabolism*; D012995:Solubility
chemical_list: D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor; D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go