[Effects of basic fibroblast growth factor on the proliferation of human salivary adenoid cystic carcinoma cell line ACC-2 and extracellular signal-regulated kinase, Cyclin D1, p2waf/cip1 signaling pathway].
OBJECTIVE
To investigate the effects of basic fibroblast growth factor (bFGF) on the proliferation of human salivary adenoid cystic carcinoma (ACC) cell line ACC-2 in vitro.


RESULTS
The effect of ectogenic bFGF on proliferation of ACC-2 was observed by MTT assay. Extracellular signal-regulated kinase (ERK) activity was measured by immuno-precipitation. p-ERK1/2, Cyclin D1 and p21waf/cip1 expression were assessed by Western blot.


RESULTS
bFGF could enhance the proliferation of ACC-2. Stimulated by bFGF, the proliferation ratio increased significantly. The intracellular ERK activity, p-ERK1/2 and Cyclin D1 expression were increased, while p21waf/cip1 expression was inhibited by different concentrations of bFGF. The above effects of bFGF could be attenuated by MEK inhibitor U0126.


CONCLUSIONS
bFGF stimulates the proliferation of ACC-2 in a dose dependent manner. The proliferation effect of bFGF may be due to up-regulating ERK, Cyclin D1 and p21waf/cip1 signaling pathway. This research can help us to explore a new pathogenesis and therapy of the ACC.
Ding
Lei
L
Department of Stomatology, Tongji Hospital of Huazhong University of Science and Technology, Wuhan 430030, China.
Zhu
Sheng-Rong
SR
Xie
San-Xiang
SX
Wu
Xiang-Bing
XB
D015153:Blotting, Western; D003528:Carcinoma, Adenoid Cystic; D002460:Cell Line; D049109:Cell Proliferation; D019938:Cyclin D1; D048049:Extracellular Signal-Regulated MAP Kinases; D016222:Fibroblast Growth Factor 2; D006801:Humans
D016222:Fibroblast Growth Factor 2; D019938:Cyclin D1; D048049:Extracellular Signal-Regulated MAP Kinases