Neem leaf glycoprotein helps to generate carcinoembryonic antigen specific anti-tumor immune responses utilizing macrophage-mediated antigen presentation.
In an objective to generate effective carcinoembryonic antigen (CEA) specific anti-tumor immune response in Swiss mice, CEA was presented using macrophages with adjuvant help from neem leaf glycoprotein (NLGP). Such vaccination generates significantly higher antibody (IgG2a) and T cell response than immunization protocol without NLGP. NLGP controls the function of both B cells and macrophages by altering the expressions of various regulatory molecules, like, CD19, CD11b, etc. NLGP also directs CEA vaccination towards Th1 bias, by modulating cytokine secretion. This NLGP-generated anti-CEA immune response would be effective as a vaccine to lyse CEA(+) tumors in vitro and in vivo.
Sarkar
Koustav
K
Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026, West Bengal, India.
Bose
Anamika
A
Chakraborty
Krishnendu
K
Haque
Enamul
E
Ghosh
Diptendu
D
Goswami
Shyamal
S
Chakraborty
Tathagata
T
Laskar
Subrata
S
Baral
Rathindranath
R
D000276:Adjuvants, Immunologic; D000818:Animals; D000912:Antibodies, Neoplasm / Q000097:blood; D017951:Antigen Presentation; D031601:Azadirachta / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000276:immunology*; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology
D000276:Adjuvants, Immunologic; D000912:Antibodies, Neoplasm; D002272:Carcinoembryonic Antigen; D006023:Glycoproteins; D010940:Plant Proteins