title: The LDL receptor-related protein can form homo-dimers in neuronal cells.
abstract: The ability of the low density lipoprotein receptor-related protein (LRP) to form homo-dimers was studied in mouse neuroblastoma and human neuroglioma cells as well as in primary cortical cultures from adult mouse brain. Homo-dimerization of LRP light chain (LC) was shown by several methods including co-immunoprecipitation, fluorescence lifetime imaging microscopy, and bimolecular fluorescence complementation assay. The requirement of intact NPXY motifs of LRP LC for homo-dimerization was ruled out by co-immunoprecipitation assay.
authors.affiliation: MassGeneral Institute for Neurodegenerative Disease, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Makarova
authors.forename: Kathryn K
authors.initials: KK
authors.lastname: Bercury
authors.forename: Kenneth W
authors.initials: KW
authors.lastname: Adams
authors.forename: Daniel
authors.initials: D
authors.lastname: Joyner
authors.forename: Meihua
authors.initials: M
authors.lastname: Deng
authors.forename: Robert
authors.initials: R
authors.lastname: Spoelgen
authors.forename: Mirjam
authors.initials: M
authors.lastname: Koker
authors.forename: Dudley K
authors.initials: DK
authors.lastname: Strickland
authors.forename: Bradley T
authors.initials: BT
authors.lastname: Hyman
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004622:Embryo, Mammalian; D004705:Endocytosis; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D026503:Low Density Lipoprotein Receptor-Related Protein-1 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D017386:Sequence Homology, Amino Acid; D014162:Transfection / Q000379:methods
chemical_list: D026503:Low Density Lipoprotein Receptor-Related Protein-1; D049452:Green Fluorescent Proteins