Bioreactor cultivation enhances NTEB formation and differentiation of NTES cells into cardiomyocytes.
Autogenic embryonic stem cells established from somatic cell nuclear transfer (SCNT) embryos have been proposed as unlimited cell sources for cell transplantation-based treatment of many genetic and degenerative diseases, which can eliminate the immune rejection that occurs after transplantation. In the present study, pluripotent nuclear transfer ES (NTES) cell lines were successfully established from different strains of mice. One NTES cell line, NT1, with capacity of germline transmission, was used to investigate in vitro differentiation into cardiomyocytes. To optimize differentiation conditions for mass production of embryoid bodies (NTEBs) from NTES cells, a slow-turning lateral vessel (STLV) rotating bioreactor was used for culturing the NTES cells to produce NTEBs compared with a conventional static cultivation method. Our results demonstrated that the NTEBs formed in STLV bioreactor were more uniform in size, and no large necrotic centers with most of the cells in NTEBs were viable. Differentiation of the NTEBs formed in both the STLV bioreactor and static culture into cardiomyocytes was induced by ascorbic acid, and the results demonstrated that STLV-produced NTEBs differentiated into cardiomyocytes more efficiently. Taken together, our results suggested that STLV bioreactor provided a more ideal culture condition, which can facilitate the formation of better quality NTEBs and differentiation into cardiomyocytes more efficiently in vitro.
Lü
Shuanghong
S
Department of Tissue Engineering, Beijing Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing 100850, People's Republic of China.
Liu
Sheng
S
He
Wenjun
W
Duan
Cuimi
C
Li
Yanmin
Y
Liu
Zhiqiang
Z
Zhang
Ye
Y
Hao
Tong
T
Wang
Yanmeng
Y
Li
Dexue
D
Wang
Changyong
C
Gao
Shaorong
S
D000818:Animals; D019149:Bioreactors; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D002470:Cell Survival; D004622:Embryo, Mammalian / Q000166:cytology / Q000502:physiology; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000502:physiology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology; D053652:Nuclear Transfer Techniques; D039904:Pluripotent Stem Cells / Q000166:cytology / Q000502:physiology