Observations of metabolite formation and variable yield in thiodiglycol biodegradation process : impact on reactor design.
The complete microbial degradation of thiodiglycol (TDG), the primary hydrolysis product of sulfur mustard, by Alcaligenes xylosoxydans ssp. xylosoxydans (SH91) was accomplished in laboratory-scale stirredtank reactors. An Andrews substrate inhibition model was used to describe the cell growth. The yield factor was not constant, but a relationship with initial substrate concentration has been developed. Using a substrate-inhibition and variable-yield kinetic model, we can describe the cell growth and substrate consumption in batch and repeated batch fermentations. Several reactor-operating modes successfully degrade TDG concentration to below 0.5 g/L. According to the experimental results, the two-stage repeated batch operation has the best degradation efficiency, and it also can degrade 500 mM TDG (= 60 g/L) to 5 mM (= 0.7 g/L) in <5 d. A hypothesis for explaining variable-yield and byproduct formation based on the capacity and utilization of metabolic loads is presented.
Lee
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Center for Agricultural Biotechnology, University of Maryland Biotechnology Institute.
Weigand
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Bentley
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