RNA extraction from various recalcitrant plant tissues with a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment.
High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds of Lotus japonicus and rice, sweet potato tuber, banana fruit). This protocol greatly reduced the time required for RNA extraction.
Suzuki
Yuji
Y
Laboratory of Plant Environmental Responses, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. ysuzuki@biochem.tohoku.ac.jp
Mae
Tadahiko
T
Makino
Amane
A
D008725:Bis-Trimethylammonium Compounds; D002021:Buffers; D002725:Chloroform; D006146:Guanidines; D008722:Methods; D019800:Phenol; D018514:Plant Structures / Q000737:chemistry; D018749:RNA, Plant / Q000302:isolation & purification*; D013861:Thiocyanates
D008725:Bis-Trimethylammonium Compounds; D002021:Buffers; D006146:Guanidines; D018749:RNA, Plant; D013861:Thiocyanates; C525618:cethyltrimethylammonium chloride; D019800:Phenol; C054436:guanidine thiocyanate; D002725:Chloroform