[Effect of METH1 gene transfection on the proliferation of rabbit's ear scar].
OBJECTIVE
To investigate the effect of METH1 gene transfection on fibroblast proliferation and I, III collagen synthesis in rabbit ear scar.


METHODS
The hypertrophic scar model on the rabbit ears was reproduced. 10 days after epithelization, Ad-METH1 was injected into the scar tissue. 30 days later, the effect of METH1 gene transfection on the angiogenesis, fibroblast proliferation and the ratio of collagen I/III in the scar tissue was detected by microcirculation microscope, AgNOR particle count and collagen dyeing.


RESULTS
30 days after injection of Ad-METH1, angiogenesis, fibroblast proliferation and the ratio of collagen I/III in the scar tissue were obviously suppressed.


CONCLUSIONS
Early application of Ad-METH1 after epithelization can markedly inhibit the formation of the hypertrophic scar.
Song
Bao-Qiang
BQ
Department of Plastic Surgery, Xijing Hospital, the Fourth Military Medical University. Xi'an 710032, China.
Lu
Kai-Hua
KH
Guo
Shu-Zhong
SZ
Zhang
Yang
Y
Peng
Pai
P
Ma
Fu-Cheng
FC
Li
Hui-Yuan
HY
D051722:ADAM Proteins / Q000235:genetics*; D020533:Angiogenesis Inhibitors / Q000235:genetics*; D000818:Animals; D017439:Cicatrix, Hypertrophic / Q000235:genetics / Q000473:pathology*; D004195:Disease Models, Animal; D004423:Ear / Q000473:pathology; D004431:Ear, External / Q000473:pathology; D005260:Female; D008297:Male; D008833:Microcirculation; D009389:Neovascularization, Pathologic; D011817:Rabbits; D014162:Transfection; D014945:Wound Healing
D020533:Angiogenesis Inhibitors; D051722:ADAM Proteins