title: Large-scale production of monoclonal antibodies in suspension culture.
abstract: Monoclonal antibodies are being manufactured for clinical trials in suspension culture at the 1300-L scale. Suspension culture offers some advantages relative to high-density mammalian cell culture methods; in particular, the ability to closely monitor the behavior of cells in a homogeneous environment. Computer control and on-line mass spectrography of exit gases provide instantaneous information about the culture metabolic activity. Air sparging and agitation by marine impeller provide aeration sufficient to maintain a constant dissolved oxygen tension at cell concentrations up to 5.0 x 10(6) cells/mL without causing apparent cell damage.
authors.affiliation: Eli Lilly and Company, Lilly Research Laboratories, Indianapolis, Indiana 46285, USA.
authors.forename: M P
authors.initials: MP
authors.lastname: Backer
authors.forename: L S
authors.initials: LS
authors.lastname: Metzger
authors.forename: P L
authors.initials: PL
authors.lastname: Slaber
authors.forename: K L
authors.initials: KL
authors.lastname: Nevitt
authors.forename: G B
authors.initials: GB
authors.lastname: Boder