title: Interaction of Salmonella enterica serovar Typhi with cultured epithelial cells: roles of surface structures in adhesion and invasion.
abstract: In this study we investigate the ability of Salmonella enterica serovar Typhi (S. Typhi) surface structures to influence invasion and adhesion in epithelial cell assay systems. In general, S. Typhi was found to be less adherent, invasive and cytotoxic than S. enterica serovar Typhimurium (S. Typhimurium). Culture conditions had little effect on adhesion of S. Typhi to cultured cells but had a marked influence on invasion. In contrast, bacterial growth conditions did not influence S. Typhi apical invasion of polarized cells. The levels of S. Typhi, but not S. Typhimurium, invasion were increased by application of bacteria to the basolateral surface of polarized cells. Expression of virulence (Vi) capsule by S. Typhi resulted in a modest reduction in adhesion, but profoundly reduced levels of invasion of non-polarized cells. However, Vi capsule expression had no affect on invasion of the apical or basolateral surfaces of polarized cells. Mutation of the staA, tcfA or pilS genes did not affect invasion or adhesion in either the presence or the absence of Vi capsule.
authors.affiliation: The Centre for Molecular Microbiology and Infection, Faculty of Life Sciences, Division of Molecular and Cell Biology, Imperial College London, London SW7 2AZ, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Bishop
authors.affiliation: The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK.
authors.forename: Deborah
authors.initials: D
authors.lastname: House
authors.affiliation: The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK.
authors.forename: Timothy
authors.initials: T
authors.lastname: Perkins
authors.affiliation: The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK.
authors.forename: Stephen
authors.initials: S
authors.lastname: Baker
authors.affiliation: The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Kingsley
authors.affiliation: The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridgeshire CB10 1SA, UK.
authors.forename: Gordon
authors.initials: G
authors.lastname: Dougan
mesh_terms: D001422:Bacterial Adhesion; D016667:Bacterial Capsules / Q000235:genetics / Q000378:metabolism*; D002460:Cell Line; D016764:Cell Polarity; D004847:Epithelial Cells / Q000382:microbiology*; D036781:Fimbriae Proteins / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D012480:Salmonella Infections / Q000382:microbiology; D012485:Salmonella typhi / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000472:pathogenicity / Q000502:physiology; D014158:Transcription, Genetic; D014435:Typhoid Fever / Q000382:microbiology*; D037521:Virulence Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D037521:Virulence Factors; D036781:Fimbriae Proteins