title: Cyclin A probes by means of intermolecular sensitization of terbium-chelating peptides.
abstract: Intermolecular sensitization of lanthanide ions was effectively implemented in the development of fluorescent sensors targeting cyclin A. A chelating unit has been conjugated to peptides containing a known cyclin A binding motif (CBM). Upon interaction of the modified terbium-chelating peptides with the cyclin A substrate recruitment groove, the Tb3+ ion is placed in the vicinity of the Trp217 side chain, which results in efficient intermolecular terbium sensitization and specific long wavelength fluorescent emission from the metal center.
authors.affiliation: Departamento de Química Orgánica, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
authors.forename: Elena
authors.initials: E
authors.lastname: Pazos
authors.forename: Daniel
authors.initials: D
authors.lastname: Torrecilla
authors.forename: Miguel
authors.initials: M
authors.lastname: Vázquez López
authors.forename: Luis
authors.initials: L
authors.lastname: Castedo
authors.forename: José L
authors.initials: JL
authors.lastname: Mascareñas
authors.forename: Anxo
authors.initials: A
authors.lastname: Vidal
authors.forename: M Eugenio
authors.initials: ME
authors.lastname: Vázquez
mesh_terms: D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods; D002614:Chelating Agents / Q000737:chemistry; D018360:Crystallography, X-Ray; D019925:Cyclin A / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D019925:Cyclin A; D005456:Fluorescent Dyes; D006573:Heterocyclic Compounds, 1-Ring; D009842:Oligopeptides; D013725:Terbium; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid