Enhanced soybean infection by the legume "super-virulent" Agrobacterium tumefaciens strain KAT23.
Agrobacterium tumefaciens KAT23 harbors a nopaline-type Ti plasmid and is "super-virulent" to soybean (Glycine max) and other leguminous plants. The right and left border sequences of the essential cis-element for T-DNA transfer were removed in order to utilize the high infectivity of this strain in an Agrobacterium-mediated soybean transformation system. The resulting strain, named Soy2, showed no oncogenic activity. After inoculation with disarmed Soy2 harboring binary vector pIG121-Hm and pCAMBIA-WR, soybean epicotyls exhibited high beta-glucuronidase activities, with efficiencies higher than EHA105, an A. tumefaciens strain widely used in making transgenic plants.
Yukawa
Kiyoshi
K
Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata 940-2188, Japan.
Kaku
Hisatoshi
H
Tanaka
Hiroshi
H
Koga-Ban
Yasunori
Y
Fukuda
Masao
M
D016960:Agrobacterium tumefaciens / Q000235:genetics* / Q000472:pathogenicity; D007887:Fabaceae; D005822:Genetic Vectors; D030821:Plants, Genetically Modified; D013025:Glycine max / Q000235:genetics / Q000382:microbiology*; D014170:Transformation, Genetic