[Production of hepatitis B virus core particles protein in plants, by using cowpea mosaic virus-based vector].
The core antigen of hepatitis B virus (HBcAg) has attracted considerable attention as a carrier for antigenic sequences for various diagnostic and vaccine applications. The hepatitis B core protein has been expressed in different expression systems. At present, for reasons of cost, scale, and safety, the plant-based expression systems are attracting increasing interest. The expression and assembly for the hepatitis B core protein were investigating in N. benthamiana plants using the new expression system based on deleted version of cowpea mosaic virus RNA-2. Analysis of HBcAg expression revealed that the core protein expressed in plants and could self-assemble into virus-like particles. Virus-like particles could be purified by differential and sucrose gradient centrifugation. This expression system has the advantage of biocontainment and can be used for the rapid production of HBcAg virus-like particles for immunological and vaccine applications.
Meshcheriakova
Iu A
IuA
El'darov
M A
MA
Beales
L
L
Skriabin
K G
KG
Lomonossoff
G P
GP
D017798:Comovirus / Q000235:genetics*; D015870:Gene Expression; D005822:Genetic Vectors; D006512:Hepatitis B Core Antigens / Q000096:biosynthesis* / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis; D014026:Nicotiana / Q000378:metabolism*
D006512:Hepatitis B Core Antigens; D012367:RNA, Viral; D011994:Recombinant Proteins