title: The large-scale immobilization of Penicillium chrysogenum: batch and continuous operation in an air-lift reactor.
abstract: A temperature-sensitive cell division cycle mutant of Penicillium chrysogenum P2 has been immobilized on Celite and grown in a 250-320-L working volume air-lift fermenter. The ability to uncouple growth and penicillin synthesis by raising the temperature to 30 degrees C also overcame the problem of the free cell mass which appeared after 300 h operation with the parent organism. After 500 h operation, penicillin and ACV dimer were still being synthesized.
authors.affiliation: SERC Centre for Biochemical Engineering, Department of Chemical and Biòchemical Engineering, University College London, Torrington Place, Loñdon WC1E 7JE, United Kingdom.
authors.forename: T
authors.initials: T
authors.lastname: Keshavarz
authors.forename: R
authors.initials: R
authors.lastname: Eglin
authors.forename: E
authors.initials: E
authors.lastname: Walker
authors.forename: C
authors.initials: C
authors.lastname: Bucke
authors.forename: G
authors.initials: G
authors.lastname: Holt
authors.forename: A T
authors.initials: AT
authors.lastname: Bull
authors.forename: M D
authors.initials: MD
authors.lastname: Lilly