title: Action pattern of alpha-amylase from Aspergillus oryzae in concentrated media.
abstract: The influence of concentrated maltotetraose solutions on fungal alpha-amylase activity and specificity has been determined. It has been found that the enzyme is not inhibited by 500 g/L substrate concentration and that transglycosylation reactions are increased with rising substrate concentration. The amount of oligosaccharides of polymerization degree higher than maltotetraose reaches 20% (w/w). Moreover, the effect of polyhydric alcohols, known as water activity depressors has been analyzed: the presence of polyols does not modify the amount of transglycosylation products, but changes the hydrolysis pattern by favoring the formation of low polymerization degree oligosaccharides.
authors.affiliation: Département de Génie Biochimique et Alimentaire, UA-CNRS 544, Institut National des Sciences Appliquées, Avenue de Rangueil, F-31077 Toulouse, France.
authors.forename: M
authors.initials: M
authors.lastname: Graber
authors.forename: D
authors.initials: D
authors.lastname: Combes