Co-culture of human gastric endoscopic biopsies with Helicobacter pylori: a simple method for studying early phases of bacteria-host interaction.
Gastric biopsies obtained through endoscopy from patients uninfected by Helicobacter pylori were co-cultured with an H. pylori strain. According to tissue and H. pylori viability, interleukin 8 was increased in biopsy homogenate and supernatant after 12-36h culture. This simple method is suitable to investigate early phases of bacteria-host interaction.
Pellicanò
Antonella
A
Dipartimento di Medicina Sperimentale e Clinica, Università di Catanzaro "Magna Graecia", Campus Universitario di Germaneto, Viale Europa 88100 Catanzaro, Italy.
Leone
Isabella
I
Imeneo
Maria
M
Amorosi
Andrea
A
Luzza
Francesco
F
D000328:Adult; D001706:Biopsy; D018920:Coculture Techniques; D016145:Endoscopy, Digestive System; D004847:Epithelial Cells / Q000382:microbiology; D005260:Female; D016480:Helicobacter pylori / Q000254:growth & development* / Q000302:isolation & purification; D054884:Host-Pathogen Interactions; D006801:Humans; D008297:Male; D008875:Middle Aged; D009924:Organ Culture Techniques / Q000379:methods; D011706:Pyloric Antrum / Q000166:cytology / Q000254:growth & development* / Q000382:microbiology*