title: Peptide substrate for caspase-3 with 2-aminoacridone as reporting group.
abstract: Synthesis and properties of a new fluorescent/fluorogenic substrate Ac-DEVD-AMAC for caspase-3 are reported. The substrate is obtained by conventional Fmoc-based solid phase peptide synthesis and its properties are investigated with regard to fluorescence, sensitivity, applicability and kinetic constants. A non-traditional approach to assay the proteases activity using 2-aminoacridone labeled peptides is proposed. This approach utilizes the decrease of fluorescence intensity of a sample as a measure for the enzyme activity.
authors.affiliation: Department of Chemistry and Biochemistry, Medical University of Sofia, 2 Zdrave str, 1431 Sofia, Bulgaria. lozanov@medfac.acad.bg
authors.forename: Valentin
authors.initials: V
authors.lastname: Lozanov
authors.forename: Ivaylo P
authors.initials: IP
authors.lastname: Ivanov
authors.forename: Bistra
authors.initials: B
authors.lastname: Benkova
authors.forename: Vanio
authors.initials: V
authors.lastname: Mitev
mesh_terms: D054831:Acridones / Q000138:chemical synthesis / Q000737:chemistry*; D000609:Aminoacridines / Q000138:chemical synthesis / Q000737:chemistry*; D053148:Caspase 3 / Q000032:analysis* / Q000737:chemistry; D002460:Cell Line; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D054831:Acridones; D000609:Aminoacridines; D005456:Fluorescent Dyes; D009842:Oligopeptides; C540557:acetyl-aspartyl-glutamyl-valyl-aspartyl-2-aminoacridone; C072515:2-aminoacridone; D053148:Caspase 3