The electrochemical regeneration of NAD(+) revisited.
An actual yield of 99.97% (i.e., 3000 potential regenerating cycles) has been obtained for the electrochemical regeneration of NAD(+). The deactivation of the coenzyme is not related to the number of cycles; it is only time dependent. For biotechnological applications, these results show that electrochemical regeneration can compete with enzymatic regeneration for selective oxidation by dehydrogenases.
Laval
J M
JM
Laboratoire Technologie Enzymatique, U.A. CNRS No. 523 Université de Technologie de Compiègne, BP 233, 60206 Compiègne Cedex, France.
Bourdillon
C
C
Moiroux
J
J