title: Induction of suppressor of cytokine signaling-3 in astrocytes of the rat hippocampus following transient forebrain ischemia.
abstract: We investigated the spatiotemporal expression of suppressor of cytokine signaling-3 (SOCS-3) in the rat hippocampus following transient forebrain ischemia using in situ hybridization and reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Messenger RNA for SOCS-3 was constitutively expressed in neurons of the pyramidal cell and granule cell layers in control animals; however, significant induction was detected in reactive astrocytes preferentially located in the CA1 and the dentate hilar regions of the ischemic hippocampus. SOCS-3 mRNA was induced within 3 days of ischemia and maintained for more than 2 weeks. The in situ hybridization data agreed with the semiquantitative RT-PCR analysis. These results demonstrate SOCS-3 induction occurs in reactive astrocytes of the post-ischemic hippocampus, suggesting that SOCS-3 is involved in regulating the astroglial reaction to an ischemic insult.
authors.affiliation: Department of Anatomy, College of Medicine, The Catholic University of Korea, 505 Banpo-dong, Socho-gu, 137-701 Seoul, Republic of Korea.
authors.forename: Jeong-Sun
authors.initials: JS
authors.lastname: Choi
authors.forename: Yoo-Jin
authors.initials: YJ
authors.lastname: Shin
authors.forename: Jung-Ho
authors.initials: JH
authors.lastname: Cha
authors.forename: Ha-Young
authors.initials: HY
authors.lastname: Kim
authors.forename: Jae-Youn
authors.initials: JY
authors.lastname: Choi
authors.forename: Myung-Hoon
authors.initials: MH
authors.lastname: Chun
authors.forename: Mun-Yong
authors.initials: MY
authors.lastname: Lee
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002135:Calcium-Binding Proteins / Q000032:analysis / Q000378:metabolism; D004268:DNA-Binding Proteins; D004195:Disease Models, Animal; D005455:Fluorescent Antibody Technique; D005904:Glial Fibrillary Acidic Protein / Q000032:analysis / Q000378:metabolism; D005911:Gliosis / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D006624:Hippocampus / Q000378:metabolism* / Q000473:pathology; D020925:Hypoxia-Ischemia, Brain / Q000378:metabolism* / Q000473:pathology; D002546:Ischemic Attack, Transient / Q000378:metabolism / Q000473:pathology; D008297:Male; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins / Q000032:analysis / Q000378:metabolism; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: C100745:Aif1 protein, rat; D015415:Biomarkers; D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D009419:Nerve Tissue Proteins; C492380:NeuN protein, mouse; D009687:Nuclear Proteins; D012333:RNA, Messenger; C491410:Socs3 protein, rat; D000071223:Suppressor of Cytokine Signaling 3 Protein; D050826:Suppressor of Cytokine Signaling Proteins