Prolonged morphine application modulates Bax and Hsp70 levels in primary rat neurons.
Morphine has been used for pain treatment with a long history. Some data suggest that morphine is toxic to neurons and induces apoptosis, while other evidence shows that morphine could have beneficial effects against cell death. To determine how morphine affects pro-apoptotic protein Bax and molecular chaperone Hsp70, different concentrations of morphine were examined. Our results show that prolonged morphine administration for 5 days at 1microM concentration protects against serum deprivation induced cell death in rat primary neurons. Morphine treatment decreases Bax and Hsp70 levels in cultured rat primary neurons, suggesting morphine may have a protective role in staurosporine and serum deprivation induced cytotoxicity.
Chen
Qiuyue
Q
Laboratory of Neurobiology and State Key Laboratory of Biomembrane and Membrane Biotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
Cui
Jia
J
Zhang
Yan
Y
Yu
Long-Chuan
LC
D000701:Analgesics, Opioid / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000831:Animals, Newborn; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D051017:Apoptosis Regulatory Proteins / Q000187:drug effects / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D001921:Brain / Q000166:cytology / Q000254:growth & development / Q000378:metabolism; D016923:Cell Death / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D016895:Culture Media, Serum-Free / Q000633:toxicity; D019610:Cytoprotection / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004334:Drug Administration Schedule; D004791:Enzyme Inhibitors / Q000633:toxicity; D050884:HSP72 Heat-Shock Proteins / Q000187:drug effects / Q000378:metabolism*; D009020:Morphine / Q000378:metabolism / Q000494:pharmacology*; D009270:Naloxone / Q000494:pharmacology; D009292:Narcotic Antagonists / Q000494:pharmacology; D009475:Neurons, Afferent / Q000187:drug effects / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000378:metabolism / Q000494:pharmacology*; D051381:Rats; D019311:Staurosporine / Q000633:toxicity; D051028:bcl-2-Associated X Protein / Q000187:drug effects / Q000378:metabolism*
D000701:Analgesics, Opioid; D051017:Apoptosis Regulatory Proteins; D016895:Culture Media, Serum-Free; D004791:Enzyme Inhibitors; D050884:HSP72 Heat-Shock Proteins; D009292:Narcotic Antagonists; D018696:Neuroprotective Agents; D051028:bcl-2-Associated X Protein; D009270:Naloxone; D009020:Morphine; D019311:Staurosporine