Anti-inflammatory and cicatrizing activities of a carbohydrate fraction isolated from sugary kefir.
Kefir is an association of microrganisms generally grown in milk, with known probiotic activities identified from its soured suspensions. Aqueous media are also able to grow kefir, but little is known about the probiotic properties of its fermented products. This work aimed to evaluate some probiotic properties of a carbohydrate fraction isolated from sugary kefir (sugary kefir carbohydrate [SKC]). Anti-inflammatory activity of the isolated fraction of carbohydrate was tested both in vitro (cellular respirometry and macrophage culture) and in vivo (50% effective dose, rat paw edema, vascular permeability, and cicatrizing test). The results indicated no significant difference for oxygen uptake or macrophage culture between control and test groups. Rat paw edema, however, showed a significant inhibitory activity by 30 +/- 4% and 54 +/- 8% (P < .001) for carrageenan and dextran, respectively. In the cicatrizing test, animals treated with SKC cream also presented less trauma after treatments as compared to the negative control group (P < .05). The overall data suggested the SKC as a natural product that could be used as a constituent of an anti-inflammatory compound.
Moreira
M E C
ME
Laboratório de Fitofármacos, Universidade José do Rosário Vellano, Unifenas, Brazil.
Dos Santos
M H
MH
Zolini
G P P
GP
Wouters
A T B
AT
Carvalho
J C T
JC
Schneedorf
J M
JM
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002241:Carbohydrates / Q000008:administration & dosage / Q000302:isolation & purification* / Q000494:pharmacology*; D002478:Cells, Cultured; D002921:Cicatrix / Q000139:chemically induced*; D043302:Cultured Milk Products / Q000737:chemistry* / Q000382:microbiology; D004487:Edema / Q000188:drug therapy; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010101:Oxygen Consumption / Q000187:drug effects; D019936:Probiotics / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D000893:Anti-Inflammatory Agents; D002241:Carbohydrates