title: Chronocoulometric techniques in the study of computerized enzyme electrode systems.
abstract: The electrochemical transient of a two-substrate enzyme electrode was studied theoretically and experimentally. Operation of such electrodes in the chronocoulometric mode leads to increased electrode sensitivity and makes possible the retrieval of useful information on transport and kinetics parameters. Digital simulation was used to solve the kinetics and transport equations and to produce the theoretical chronocoulometric response. A glucose electrode based on glucose oxidase crosslinked to different matrices was tested with air oxygen and p-benzoquinone as the cosubstrate. A computerized electrochemical system was employed for electrode potential control and data acquisition and analysis.
authors.affiliation: Center for Biotechnology, George S Wise Faculty of Life Science, Tel-Aviv University, Ramat Aviv 69978, Israel.
authors.forename: J
authors.initials: J
authors.lastname: Rishpon