title: Molecular recognition of nitrated fatty acids by PPAR gamma.
abstract: Peroxisome proliferator activated receptor-gamma (PPAR gamma) regulates metabolic homeostasis and adipocyte differentiation, and it is activated by oxidized and nitrated fatty acids. Here we report the crystal structure of the PPAR gamma ligand binding domain bound to nitrated linoleic acid, a potent endogenous ligand of PPAR gamma. Structural and functional studies of receptor-ligand interactions reveal the molecular basis of PPAR gamma discrimination of various naturally occurring fatty acid derivatives.
authors.affiliation: Department of Pharmaceutical Sciences, Center for Pharmacogenetics, 709 Salk Hall, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA. yol21@pitt.edu
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.forename: Jifeng
authors.initials: J
authors.lastname: Zhang
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Schopfer
authors.forename: Dariusz
authors.initials: D
authors.lastname: Martynowski
authors.forename: Minerva T
authors.initials: MT
authors.lastname: Garcia-Barrio
authors.forename: Amanda
authors.initials: A
authors.lastname: Kovach
authors.forename: Kelly
authors.initials: K
authors.lastname: Suino-Powell
authors.forename: Paul R S
authors.initials: PR
authors.lastname: Baker
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Freeman
authors.forename: Y Eugene
authors.initials: YE
authors.lastname: Chen
authors.forename: H Eric
authors.initials: HE
authors.lastname: Xu
mesh_terms: D017667:Adipocytes / Q000166:cytology; D002454:Cell Differentiation; D018360:Crystallography, X-Ray / Q000379:methods; D005227:Fatty Acids / Q000737:chemistry*; D006801:Humans; D008024:Ligands; D019787:Linoleic Acid / Q000737:chemistry; D008958:Models, Molecular; D008968:Molecular Conformation; D009584:Nitrogen / Q000737:chemistry; D010100:Oxygen / Q000737:chemistry; D047495:PPAR gamma / Q000737:chemistry / Q000378:metabolism*; D017434:Protein Structure, Tertiary
chemical_list: D005227:Fatty Acids; D008024:Ligands; D047495:PPAR gamma; D019787:Linoleic Acid; D009584:Nitrogen; D010100:Oxygen