title: Sustained delayed-type hypersensitivity reaction after in vivo priming but successful induction of unresponsiveness after adoptive transfer of CD4+ effector T cells.
abstract: Potential reasons for weak effects of oral tolerance in the primed immune system are still under discussion. In the present study, impacts of oral antigen uptake were studied in adoptive transfer models using T cell receptor transgenic CD4(+) T cells allowing analysis of antigen-specific donor cells on single cell level. After in vivo priming and subsequent feeding, an antigen-specific delayed-type hypersensitivity reaction was sustained. Concomitantly, donor cells preferentially found in the draining lymph nodes remained at equal numbers. In contrast, adoptively transferred Th1 cells that migrated preferentially into spleen and liver became fewer upon feeding accompanied by a suppressed delayed-type hypersensitivity reaction. Thus, antigen-experienced cells did not seem to become generally resistant to tolerogenic stimuli. Our data suggest that besides a permanent inflammatory stimulus provided by the persisting antigen, diverse tissue distribution of in vivo-induced compared to adoptively transferred effector cells might interfere with oral tolerance in the experienced immune system.
authors.affiliation: Experimentelle Rheumatologie, Charité Campus Mitte, Berlin, Germany.
authors.forename: Friderike
authors.initials: F
authors.lastname: Blumenthal-Barby
authors.forename: Arnhild
authors.initials: A
authors.lastname: Schrage
authors.forename: Katharina
authors.initials: K
authors.lastname: Eulenburg
authors.forename: Martin
authors.initials: M
authors.lastname: Zeitz
authors.forename: Alf
authors.initials: A
authors.lastname: Hamann
authors.forename: Katja
authors.initials: K
authors.lastname: Klugewitz
mesh_terms: D000284:Administration, Oral; D019264:Adoptive Transfer; D000818:Animals; D015704:CD4 Antigens / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000637:transplantation; D049109:Cell Proliferation; D006968:Hypersensitivity, Delayed / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000008:administration & dosage / Q000276:immunology; D010641:Phenotype; D016176:T-Lymphocyte Subsets / Q000276:immunology* / Q000637:transplantation; D018417:Th1 Cells / Q000276:immunology / Q000637:transplantation; D014018:Tissue Distribution
chemical_list: D015704:CD4 Antigens; D010047:Ovalbumin