PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells.
Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including alpha-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.
Jang
Chan Ho
CH
Department of Animal Science and Biotechnology, Kyungpook National University, Daegu, South Korea.
Lee
In Ae
IA
Ha
Young Ran
YR
Lim
Jinkyu
J
Sung
Mi-Kyung
MK
Lee
Sung-Joon
SJ
Kim
Jong-Sang
JS
D000872:Anthocyanins / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D040901:Proteomics
D000872:Anthocyanins; D000975:Antioxidants; D015415:Biomarkers; D006861:Hydrogen Peroxide; D010735:Phosphoglycerate Kinase; C017185:delphinidin