title: Comprehensive two-dimensional high-performance liquid chromatography system with immobilized liposome chromatography column and reversed-phase column for separation of complex traditional Chinese medicine Longdan Xiegan Decoction.
abstract: A comprehensive two-dimensional HPLC system with an immobilized liposome chromatography (ILC) column in conjunction with an RP column (in tandem) was developed for the screening and analysis of the membrane-permeable compounds in a traditional Chinese medicine prescription Longdan Xiegan Decoction (LXD). More than 50 components in LXD were resolved using the developed separation system. Eight flavonoids and two iridoids were identified interacting with the ILC column; a system that mimics biomembranes. The results show that the developed comprehensive two-dimensional chromatography system can be used for identifying membrane permeable natural products in complex matrixes such as extracts of traditional Chinese medicine prescriptions.
authors.affiliation: Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Liang
authors.initials: L
authors.lastname: Kong
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Lei
authors.forename: Lianghai
authors.initials: L
authors.lastname: Hu
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
authors.forename: Ed
authors.initials: E
authors.lastname: Welbeck
authors.forename: S W Annie
authors.initials: SW
authors.lastname: Bligh
authors.forename: Zhengtao
authors.initials: Z
authors.lastname: Wang
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D039823:Iridoids / Q000032:analysis*; D008081:Liposomes; D013058:Mass Spectrometry; D008567:Membranes, Artificial
chemical_list: D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D039823:Iridoids; D008081:Liposomes; D008567:Membranes, Artificial; C526720:longdan xiegan decoction