title: Structured modeling of a microbial system: II. Experimental verification of a structured lactic acid fermentation model.
abstract: A two-compartment model for the lactic acid fermentation with Streptococcus cremoris is experimentally verified. The seven parameters of the model are determined using steady-state chemostat data at varying values of dilution rate, D, but with a constant feed concentration, s(f), of a single carbohydrate source (glucose, lactose, or galactose), and a constant feed concentration of s(Nf) of the N source. Steady-state measurements of the RNA content at different exit concentrations, s, of the carbohydrate are included to calculate kinetic parameters that determine the cell composition for varying operating conditions. The model is tested using data from a large set of steady-state and non-steady-state experiments: batch fermentations and step and pulse experiments in a chemostat. Both qualitatively and quantitatively the major features of the model are confirmed: the external substrates enter into intracellular high-energy building blocks, and lactic acid is formed as a by-product of these reactions. Cell growth depends on the fraction of active components (X(A)) of the cell and is not accompanied by lactic acid production. Possible model modifications are discussed, primarily to obtain a better description of lactic acid fermentation at nongrowth conditions.
authors.affiliation: Department of Biotechnology, Technical University of Denmark, Lyngby, Denmark.
authors.forename: J
authors.initials: J
authors.lastname: Nielsen
authors.forename: K
authors.initials: K
authors.lastname: Nikolajsen
authors.forename: J
authors.initials: J
authors.lastname: Villadsen