title: Structure of Ynk1 from the yeast Saccharomyces cerevisiae.
abstract: Nucleoside diphosphate kinase (NDPK) catalyzes the transfer of the gamma-phosphate from nucleoside triphosphates to nucleoside diphosphates. In addition to biochemical studies, a number of crystal structures of NDPK from various organisms, including both native proteins and complexes with nucleotides or nucleotide analogues, have been determined. Here, the crystal structure of Ynk1, an NDPK from the yeast Saccharomyces cerevisiae, has been solved at 3.1 A resolution. Structural analysis strongly supports the oligomerization state of this protein being hexameric rather than tetrameric.
authors.affiliation: Protein Research Institute, Tongji University, Shanghai 200092, People's Republic of China.
authors.forename: Huabing
authors.initials: H
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Bao
authors.forename: Chunhui
authors.initials: C
authors.lastname: Jiang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Yang
authors.forename: Cong Zhao
authors.initials: CZ
authors.lastname: Zhou
authors.forename: Yuxing
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D019281:Dimerization; D024101:Mitochondrial Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009701:Nucleoside-Diphosphate Kinase / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
chemical_list: D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D009701:Nucleoside-Diphosphate Kinase; C473137:YNK1 protein, S cerevisiae