title: Population balance between producing and nonproducing hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition.
abstract: Secreting and nonsecreting hybridoma populations derived from the murine hybridoma cell line 167.4G5.3 were each grown in batch culture in low serum and serum-free media. Under serum-free conditions, a secreting population gained on a predominantly nonsecreting population and competed with the existing antibody-deficient cells effectively. It was found that this competition was sensitive to state of inoculum and medium composition. We conclude that the competition between a secreting and nonsecreting, or more generally, a producing and nonproducing, population is important; the appearance of the latter may not be a significant setback in terms of expected product titer.
authors.affiliation: Cellular Biotechnology Laboratory, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.
authors.forename: A S
authors.initials: AS
authors.lastname: Chuck
authors.forename: B O
authors.initials: BO
authors.lastname: Palsson