Virion disruption by ozone-mediated reactive oxygen species.
It is well documented in the scientific literature that ozone-oxygen mixtures inactivate microorganisms including bacteria, fungi and viruses (Hoff, J.C., 1986. Inactivation of microbial agents by chemical disinfectants. EPA 600 S2-86 067. Office of Water, U.S. Environmental Protection Agency, Washington, DC; Khadre, M.A., Yousef, A.E., Kim, J.-G., 2001. Microbiological aspects of ozone applications in food: a review. J. Food Sci. 66, 1242-1252). In the current study, delivery and absorption of precisely known concentrations of ozone (in liquid media) were used to inactivate virus infectivity. An ozone-oxygen delivery system capable of monitoring and recording ozone concentrations in real time was used to inactivate a series of enveloped and non-enveloped viruses including herpes simplex virus type-1 (HHV-1, strain McIntyre), vesicular stomatitis Indiana virus (VSIV), vaccinia virus (VACV, strain Elstree), adenovirus type-2 (HAdV-2), and the PR8 strain of influenza A virus (FLUAVA/PR/8/34/H1N1; FLUAV). The results of the study showed that ozone exposure reduced viral infectivity by lipid peroxidation and subsequent lipid envelope and protein shell damage. These data suggest that a wide range of virus types can be inactivated in an environment of known ozone exposure.
Murray
Byron K
BK
Department of Microbiology and Molecular Biology, Brigham Young University, 857 WIDB, Provo, UT 84602-5253, USA. byron_murray@byu.edu
Ohmine
Seiga
S
Tomer
David P
DP
Jensen
Kendal J
KJ
Johnson
F Brent
FB
Kirsi
Jorma J
JJ
Robison
Richard A
RA
O'Neill
Kim L
KL
D000256:Adenoviridae / Q000187:drug effects / Q000648:ultrastructure; D004202:Disinfectants / Q000494:pharmacology*; D009980:Influenza A virus / Q000187:drug effects / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D010126:Ozone / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000494:pharmacology*; D018139:Simplexvirus / Q000187:drug effects / Q000648:ultrastructure; D014616:Vaccinia virus / Q000187:drug effects / Q000648:ultrastructure; D014721:Vesicular stomatitis Indiana virus / Q000187:drug effects / Q000648:ultrastructure; D010948:Viral Plaque Assay; D014771:Virion / Q000187:drug effects* / Q000648:ultrastructure; D038301:Virus Inactivation
D004202:Disinfectants; D017382:Reactive Oxygen Species; D010126:Ozone