title: One-chip sensing device (biomedical photonic LSI) enabled to assess hippocampal steep and gradual up-regulated proteolytic activities.
abstract: We developed an implantable one-chip biofluoroimaging device (termed biomedical photonic LSI; BpLSI) which enabled real-time molecular imaging with conventional electrophysiology in vivo in deep brain areas. The multimodal LSI enabled long-term sequential imaging of the fluorescence emitted by proteolysis-linked fluorogenic substrate. Using the BpLSI, we observed a process of stimulation-dependent modulation at synapse with multi-site (16 x 19 pixel) in widespread area and a high-speed video rate, and found that the gradual up-regulated proteolytic activity in a wide range of hippocampal CA1 area and the steep activity in local area, indicating that the proteolysis system is a basis for the fixation of long-term potentiation in post-excited synapses in the hippocampus. Mathematical data analysis confirmed the direct involvement of functional proteolysis for neural plasticity.
authors.affiliation: Division of Structural Cell Biology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Tamura
authors.forename: David C
authors.initials: DC
authors.lastname: Ng
authors.forename: Takashi
authors.initials: T
authors.lastname: Tokuda
authors.forename: Honda
authors.initials: H
authors.lastname: Naoki
authors.forename: Takuma
authors.initials: T
authors.lastname: Nakagawa
authors.forename: Taro
authors.initials: T
authors.lastname: Mizuno
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Hatanaka
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ishikawa
authors.forename: Jun
authors.initials: J
authors.lastname: Ohta
authors.forename: Sadao
authors.initials: S
authors.lastname: Shiosaka
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D020811:Biomedical Technology / Q000295:instrumentation*; D003374:Coumarins / Q000494:pharmacology; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods*; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D006624:Hippocampus / Q000187:drug effects / Q000502:physiology*; D007610:Kallikreins / Q000378:metabolism; D017774:Long-Term Potentiation / Q000187:drug effects / Q000502:physiology; D008297:Male; D051379:Mice; D008954:Models, Biological; D009842:Oligopeptides / Q000494:pharmacology; D012697:Serine Endopeptidases / Q000378:metabolism*; D013997:Time Factors
chemical_list: D003374:Coumarins; D009842:Oligopeptides; C036903:tertiary-butyloxycarbonyl-valyl-prolyl-arginyl-7-amino-4-methylcoumarin; D007610:Kallikreins; D012697:Serine Endopeptidases