[Initial study of Hemp seeds protein on antifatigue and the immunomodulation effects in mice].
OBJECTIVE
To study the effects of Hemp seeds protein (HSP) on antifatigue and immunnomodulation effect in mice.


METHODS
Female ICR mice at the ages of 6-8 weeks were administered with the HSP for 4 weeks at the dose of 1.32, 2.64 and 7.92 g/kg bw. After four weeks' treatment, the mice swimming time and some hiochemistry indexes were tested, such as blood lactic acid, BUN and hepatic glycogen, as well as the ratio of spleen and thymus organs index, the capacities of lymphocyte proliferation induced by ConA, DTH response, IgM-PFC number, the level of serum HC50, the indexes of the chicken red blood cells phagocytosis and the clearance rate of carbon particles were detected, and the ratio of CD4+ T helper (Th) cell in spleen were determined by the flow cytometer.


RESULTS
In comparison with the control group, the HSP could improve the the swimming time and contents of liver hepatin distinctly, and reduced the contents of blood lactic acid. Then the capacities of lymphocyte proliferation induced by ConA, DTH response, IgM-PFC number, the level of serum HC50, the indexes of the clearance rate of carbon particles and the ratio of CD4+ T helper (Th) cell in spleen increased.


CONCLUSIONS
HSP could have the ability of antifatigue and improve the immunomodulation effect in mice.
Li
Yongjin
Y
Department of Nutrition & Food Hygiene, School of Public Health, Peking University, Beijing 100083, China. lyjsod@sofu.com
Yang
Ruiyue
R
Hu
Xuefeng
X
Long
Zhu
Z
et al
D000818:Animals; D002188:Cannabis / Q000737:chemistry*; D005221:Fatigue / Q000517:prevention & control*; D005260:Female; D056747:Immunomodulation / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D008517:Phytotherapy; D010940:Plant Proteins / Q000302:isolation & purification / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*
D010940:Plant Proteins