[Transduction of rhesus bone marrow mesenchymal stem cells by recombinant baculovirus].
OBJECTIVE
To investigate whether the recombinant baculovirus (Bac-CMV-EGFP) can effectively transduce into rhesus Bone-marrow derived Mesenchymal Stem Cells (rBMSCs) in vitro, and whether there are some efficiency to the rBMSCs of viability, proliferational and differentiational capacity after recombinant baculovirus transducing.


METHODS
The rBMSCs were cultured in vitro. After passaged more than three times, the rBMSCs were transduced with various dose of baculovirus (Multiplicity Of Infection, MOI, the MOI is 50, 100, 200, 300, and 500 vector genome (vg)/cell, respectively). We used flow cytometry to detect different transductive efficiency of various dose of baculovirus to rBMSCs. Under a suitable dose of baculovirus (300 v.g/cell), we studied cell viability, proliferation and differentiation capacity, and compared results with the control.


RESULTS
Baculovirus could be transduced into rBMSCs in vitro. The transductive efficiency reached about 80% when the MOI was 300 v.g/cell, at 25 degrees C, and incubated for 4 h. Furthermore, under a higher transductive efficiency of baculovirus, there were no obvious influence to the rBMSCs of viability, proliferation and differentiation capacity compared with that of the control.


CONCLUSIONS
The baculovirus can be safely and effectively transduced into rBMSCs in vitro, without any negative efficiency to cell viability, proliferation and differentiation capacity.
Liu
Zhengshan
Z
Department of Neurology, First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China. 314711687@163.com
Zhang
Cheng
C
Lu
Xilin
X
Xu
Yongfeng
Y
Feng
Shanwei
S
Zhou
Chang
C
Li
Yong
Y
Cheng
Fei
F
D000818:Animals; D016367:Baculoviridae / Q000235:genetics* / Q000502:physiology; D001854:Bone Marrow Cells / Q000166:cytology*; D049109:Cell Proliferation; D002470:Cell Survival; D004274:DNA, Recombinant / Q000235:genetics*; D008253:Macaca mulatta; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D014161:Transduction, Genetic / Q000379:methods*
D004274:DNA, Recombinant