title: Leishmania (Leishmania) amazonensis: experimental cutaneous leishmaniasis associated with systemic amyloidosis in mice.
abstract: We infected Swiss and C57BL/6 female mice in the left hind footpad with 10(4)Leishmania (L.) amazonensis promastigotes in stationary phase. The macroscopic examination showed a nodular non-ulcerated lesion at the site of inoculation and hepatic and spleenic enlargement. Histopathologically, the primary lesion showed an extensive liquefactive necrosis and inflammatory infiltrate, mainly consisting of macrophages filled with amastigotes, and rare lymphocytes. The inflammatory reaction in liver, spleen and kidney showed amyloid deposits. Additionally, C57BL/6 had accentuated amyloidosis in both ovarian cortical and medullar region and inflammatory infiltrates in the pancreas and adrenal gland.
authors.affiliation: Laboratório de Imunomodulação e Protozoologia do Instituto Oswaldo Cruz/FIOCRUZ, Pavilhão Carlos Chagas, 3 degrees andar, Av. Brasil, 4365, Manguinhos, CEP 20045-900, Rio de Janeiro, Brazil.
authors.forename: Luiz Otávio Pereira
authors.initials: LO
authors.lastname: Carvalho
authors.forename: Kátia da Silva
authors.initials: Kda S
authors.lastname: Calabrese
authors.forename: Sylvio Celso Gonçalves
authors.initials: SC
authors.lastname: da Costa
authors.forename: Verônica Gonçalves
authors.initials: VG
authors.lastname: Mendes
authors.forename: Ana Patrícia de Carvalho
authors.initials: AP
authors.lastname: da Silva
authors.forename: Ana Caroline Ericeira
authors.initials: AC
authors.lastname: Barros
authors.forename: Solange de Araújo
authors.initials: Sde A
authors.lastname: Melo
authors.forename: Ana Lucia
authors.initials: AL
authors.lastname: Abreu-Silva
mesh_terms: D000311:Adrenal Glands / Q000473:pathology; D000686:Amyloidosis / Q000150:complications* / Q000469:parasitology / Q000473:pathology; D000818:Animals; D005260:Female; D007515:Islets of Langerhans / Q000473:pathology; D007668:Kidney / Q000473:pathology; D007894:Leishmania mexicana; D016773:Leishmaniasis, Cutaneous / Q000150:complications*; D008099:Liver / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010053:Ovary / Q000473:pathology; D013154:Spleen / Q000473:pathology