title: The total effective xenoestrogen burden, a biomarker of exposure to xenoestrogen mixtures, is predicted by the (anti)estrogenicity of its components.
abstract: Appropriate biomarkers of human exposure are required for epidemiological studies of endocrine disruption. We addressed this issue by improving a standardized method to assess the total effective xenoestrogen burden (TEXB), a biomarker of xenoestrogen exposure. Extensive separation of xenoestrogens from endogenous hormones was made in 20 adipose tissue samples by HPLC, and two eluates were separated and tested in the E-Screen bioassay. An extensive fractionation protocol was also developed. The objective of this study was to investigate predictors of TEXB by using a multiple regression model after adjusting by confounding factors. The final model included the estrogenicity of 8 out of 11 individual 1-min fractions into which the xenoestrogen eluate was split and the marital status of patients, and it explained 97% of TEXB variability, and variables. Our results indicate that TEXB of complex mixtures can be accurately predicted from the estrogenicity of a small number of components.
authors.affiliation: Department of Radiology, University of Granada, 18071-Granada, Spain. marieta@ugr.es
authors.forename: M F
authors.initials: MF
authors.lastname: Fernandez
authors.forename: C
authors.initials: C
authors.lastname: Aguilar-Garduño
authors.forename: J M
authors.initials: JM
authors.lastname: Molina-Molina
authors.forename: J P
authors.initials: JP
authors.lastname: Arrebola
authors.forename: N
authors.initials: N
authors.lastname: Olea
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis*; D001822:Body Burden; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid; D004781:Environmental Exposure / Q000032:analysis*; D004784:Environmental Monitoring / Q000379:methods*; D004785:Environmental Pollutants / Q000032:analysis* / Q000737:chemistry; D004968:Estrogens, Non-Steroidal / Q000032:analysis* / Q000737:chemistry; D005260:Female; D006801:Humans; D008875:Middle Aged; D015999:Multivariate Analysis; D015262:Xenobiotics / Q000009:adverse effects / Q000032:analysis* / Q000737:chemistry
chemical_list: D015415:Biomarkers; D004785:Environmental Pollutants; D004968:Estrogens, Non-Steroidal; D015262:Xenobiotics