title: Elevated intracellular chloride level in albino visual cortex neurons is mediated by Na-K-Cl co-transporter.
abstract: BACKGROUND
  During development the switch from a depolarizing to a hyperpolarizing action of GABA is a consequence of a decrease of the Na+-K+-2Cl- co-transporter (NKCC1, Cl--uptake) and increase of the K+-Cl- co-transporter (KCC2, Cl--extrusion) expression. However albino visual cortex neurons don't show a corresponding decrease in intracellular chloride concentration during development of the visual system as compared to pigmented animals.
  
  
  RESULTS
  Our study revealed that more cells express NKCC1 in albinos compared to pigmented rat visual cortex neurons whereas KCC2 is expressed in all cells in both strains. We determined a positive relationship between the presence of NKCC1 and an inhibitory deficit in single neurons of the albino visual cortex. After pharmacological blockade of NKCC1 function with its specific inhibitor, bumetanide, the reversal potential of electrically evoked GABAA receptor-mediated postsynaptic currents and, as a consequence, [Cl-]i in albino visual cortex neurons shifted to the pigmented rat brain value. In conclusion, our pharmacological experiments and subsequent single cell real time PCR analysis of the co-transporter mRNA demonstrated that the inhibitory deficit present in the albino visual cortical network is almost exclusively mediated by NKCC1.
  
  
  CONCLUSIONS
  Our findings suggest that blocking of NKCC1 in albino visual cortex neurons could improve processing in visual cortex and therefore might be beneficial for vision in albinos.
authors.affiliation: International Graduate School of Neuroscience, Ruhr University Bochum, FNO 01/114 Universitätsstr.150, 44801, Bochum, Germany. Dmitry.Diykov@ruhr-uni-bochum.de
authors.forename: Dmitry
authors.initials: D
authors.lastname: Diykov
authors.forename: Andrey
authors.initials: A
authors.lastname: Turchinovich
authors.forename: Georg
authors.initials: G
authors.lastname: Zoidl
authors.forename: Klaus-Peter
authors.initials: KP
authors.lastname: Hoffmann
mesh_terms: D000417:Albinism / Q000188:drug therapy / Q000235:genetics / Q000503:physiopathology; D000818:Animals; D002034:Bumetanide / Q000494:pharmacology; D002712:Chlorides / Q000032:analysis / Q000378:metabolism*; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D005071:Evoked Potentials / Q000187:drug effects / Q000502:physiology; D019706:Excitatory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D005260:Female; D053444:Inhibitory Postsynaptic Potentials / Q000187:drug effects / Q000502:physiology; D008297:Male; D008564:Membrane Potentials / Q000187:drug effects / Q000502:physiology; D009433:Neural Inhibition / Q000187:drug effects / Q000502:physiology; D017966:Pyramidal Cells / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020318:Rats, Long-Evans; D017208:Rats, Wistar; D011963:Receptors, GABA-A / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D028021:Sodium-Potassium-Chloride Symporters / Q000235:genetics / Q000502:physiology*; D013045:Species Specificity; D014793:Visual Cortex / Q000166:cytology* / Q000502:physiology; D005680:gamma-Aminobutyric Acid / Q000502:physiology
chemical_list: D002712:Chlorides; D012333:RNA, Messenger; D011963:Receptors, GABA-A; D028021:Sodium-Potassium-Chloride Symporters; D002034:Bumetanide; D005680:gamma-Aminobutyric Acid