title: [Isolation, culture and characterization of endothelial cells in infantile hemangioma].
abstract: OBJECTIVE
  To establish a method for the reliable isolation and culture of infantile hemangioma endothelial cells (HemECs) in vitro.
  
  
  METHODS
  Proliferative hemangioma specimens were digested by collagenase to form a single cell suspension. The HemECs were isolated using anti-CD31 coated dynabeads. The CD31+ cells were grown in fibronectin coated dishes. HemECs were identified by morphological characteristics and immunocytochemistry. The cells were also examined for their ability to intake LDL.
  
  
  RESULTS
  The method enabled the rapid isolation of HemECs that demonstrated typical endothelial cobblestone morphology in culture. The cells were positively stained for CD31, vWF. They also were labeled with DiI-Ac-LDL.
  
  
  CONCLUSIONS
  This technique can effectively isolate endothelial cells from the proliferative hemangiomas. These cells could be further used to research the mechanism of proliferation and degeneration of infantile hemangioma.
authors.affiliation: Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200011, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Ma
authors.forename: Xiao-Xi
authors.initials: XX
authors.lastname: Lin
authors.forename: Yun-Bo
authors.initials: YB
authors.lastname: Jin
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Xiao-Jie
authors.initials: XJ
authors.lastname: Hu
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Xu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Chuan
authors.initials: C
authors.lastname: Yang
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D042783:Endothelial Cells / Q000166:cytology*; D005434:Flow Cytometry; D018324:Hemangioma, Capillary; D006801:Humans