Development of a portable biosensor for screening neurotoxic agents in water samples.
A high sensitive portable biosensor system capable of determining the presence of neurotoxic agents in water has been developed. The system consists of (i) a screen-printed electrode with acetylcholinesterase (AChE) immobilized on it, (ii) a self-developed portable potentiostat with an analog to digital (A/D) converter and a serial interface for transferring data to a portable PC and (iii) an own designed software, developed with Lab-Windows CVI, used to record and process the measurements. The system has been developed to perform high precision amperometrical measurements with low drifts, low noise and a good reproducibility. In the configuration depicted, the percentage of AChE inhibition is proportional to the content of neurotoxic agents in a sample. This type of measurement is performed by the steady-state method from the first steady current (by a phosphate buffer solution) and the second steady current (by an enzymatic reaction produced by the addition of acetylthiocholine chloride to the solution). Validation was performed by analyzing spiked water samples containing pesticides. The design is specially suited for screening purposes, does not need sample preconcentration, is totally autonomous and suitable for the field detection of neurotoxic agents in water.
Hildebrandt
Alain
A
Department of Environmental Chemistry, IIQAB-CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain.
Ribas
Jordi
J
Bragós
Ramon
R
Marty
Jean-Louis
JL
Tresànchez
Màrius
M
Lacorte
Sílvia
S
D000110:Acetylcholinesterase / Q000737:chemistry*; D000122:Acetylthiocholine / Q000032:analysis; D001322:Autoanalysis; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D004800:Enzymes, Immobilized; D009498:Neurotoxins / Q000032:analysis* / Q000737:chemistry; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014867:Water / Q000032:analysis / Q000737:chemistry*
D004800:Enzymes, Immobilized; D009498:Neurotoxins; D014867:Water; D000122:Acetylthiocholine; D000110:Acetylcholinesterase