title: Triggering vacuum capillaries for pneumatic pumping and metering liquids in point-of-care immunoassays.
abstract: An innovative vacuum capillary pneumatic actuation concept that can be used for point-of-care testing has been investigated. The vacuum glass capillaries are encapsulated within a laminated pouch and incorporated into the fluidic card. Vacuum glass capillaries broken by external force such as finger pressure, generate the pneumatic forces to induce liquid flow in the fluidic system. The sizes of vacuum capillary play a vital role in the pumping and metering functions of the system. The luteinizing hormone (LH) chromatographic immunoassay performances in the fluidic cards show consistency comparable to that obtained by manual micropipetting. The vacuum capillary pneumatic actuation will be applied in other complex handling step bioassays and lab-on-a-chip devices.
authors.affiliation: Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu 300, Taiwan. allan_weng@itri.org.tw
authors.forename: Kuo-Yao
authors.initials: KY
authors.lastname: Weng
authors.forename: Nien-Jen
authors.initials: NJ
authors.lastname: Chou
authors.forename: Jya-Wei
authors.initials: JW
authors.lastname: Cheng
mesh_terms: D003365:Costs and Cost Analysis; D005898:Glass / Q000737:chemistry; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation*; D007986:Luteinizing Hormone / Q000032:analysis*; D019563:Mechanics; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010969:Plastics / Q000737:chemistry; D019095:Point-of-Care Systems; D014867:Water / Q000737:chemistry
chemical_list: D010969:Plastics; D014867:Water; D007986:Luteinizing Hormone