title: Minimal conjunctival limbal autograft for total limbal stem cell deficiency.
abstract: OBJECTIVE
  To report the results of one 60 degrees conjunctival limbal autograft (CLAU) combined with amniotic membrane (AM) transplantation for an eye with total limbal stem cell deficiency (LSCD).
  
  
  METHODS
  One eye of a patient with chronic total LSCD and symblepharon caused by chemical burn was subjected to symblepharon lysis, removal of pannus from corneal surface, AM transplantation to cover the conjunctival and corneal surfaces as a permanent graft, one 60 degrees CLAU to the superior limbal area, and insertion of ProKera as a temporary AM patch to cover the CLAU.
  
  
  RESULTS
  After surgery, corneal epithelialization over the AM was evident adjacent to the CLAU on day 6, progressed to pass the horizontal midline by day 11, and was completed by day 18. During a follow-up of 1 year, the corneal surface remained stable and smooth, and the stroma considerably regained clarity with regression of midstromal vascularization. The best-corrected visual acuity improved from 20/400 to 20/50. The conjunctival inflammation completely resolved, and the fornices were deep.
  
  
  CONCLUSIONS
  One 60 degrees CLAU combined with AM transplantation as both a permanent graft and a temporary patch can restore the entire corneal surface in an eye with total LSCD caused by chemical burn.
authors.affiliation: Ocular Surface Center, Miami, FL 33173, USA.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Kheirkhah
authors.forename: Vadrevu K
authors.initials: VK
authors.lastname: Raju
authors.forename: Scheffer C G
authors.initials: SC
authors.lastname: Tseng
mesh_terms: D000293:Adolescent; D000650:Amnion / Q000637:transplantation*; D002057:Burns, Chemical / Q000601:surgery*; D003228:Conjunctiva / Q000166:cytology*; D003316:Corneal Diseases / Q000601:surgery*; D004847:Epithelial Cells / Q000637:transplantation; D019573:Epithelium, Corneal / Q000637:transplantation*; D005126:Eye Burns / Q000139:chemically induced; D006801:Humans; D016850:Limbus Corneae / Q000166:cytology*; D008297:Male; D013234:Stem Cells / Q000473:pathology*; D014182:Transplantation, Autologous; D014792:Visual Acuity; D014945:Wound Healing