title: Identification of a single-nucleocapsid baculovirus isolated from Clanis bilineata tsingtauica (Lepidoptera: Sphingidae).
abstract: A nucleopolyhedrovirus isolated from infected larvae of Clanis bilineata tsingtauica was characterized. Electron microscopical studies on the ultrastructure of C. bilineata nucleopolyhedrovirus (ClbiSNPV) occlusion bodies (OBs) showed several virions (up to 16) with a single nucleocapsid packaged within a single viral envelope. The diameter of the OBs was 0.77-1.7 mum with a mean of 1.13 +/- 0.19 mum. The complete sequence of the ClbiSNPV polyhedrin (polh) gene contained 741 nucleotides, predicting a protein of 246 amino acids. Phylogenetic analyses using the complete sequence of the polh genes indicated that ClbiSNPV clusters with Group II NPVs. This is the first record of a baculovirus from C. bilineata.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Xuefu Road 301, Zhenjiang, China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Wang
authors.forename: Jianping
authors.initials: J
authors.lastname: Yi
authors.forename: Shanying
authors.initials: S
authors.lastname: Zhu
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.forename: Weide
authors.initials: W
authors.lastname: Shen
authors.forename: Wenbing
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D004279:DNA, Viral / Q000737:chemistry / Q000235:genetics; D005814:Genes, Viral; D007814:Larva / Q000821:virology; D007915:Lepidoptera / Q000821:virology*; D019251:Nucleocapsid / Q000235:genetics*; D017924:Nucleopolyhedroviruses / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins