title: Determination of phospholipids in olive oil by 31P NMR spectroscopy.
abstract: A nondestructive analytical method based on NMR spectroscopy was developed for the determination of phospholipids in olive oil. The phospholipids extracted from virgin olive oil with a mixture of ethanol/water (2:1 v/v) were identified and quantified by high resolution (31)P NMR spectroscopy. The main phospholipids found in olive oil were phosphatidic acid, lyso-phosphatidic acid, and phosphatidylinositol. Validation of the (31)P NMR methodology for quantitative analysis of phospholipids in olive oil was performed. Sensitivity was satisfactory with detection limits of 0.25-1.24 mumol /mL. In addition, the composition of fatty acids in phospholipids model compounds and those in olive oil samples was estimated by employing one- and two-dimensional (1)H NMR. The results indicated that the fatty acid composition in phospholipids and triacylglycerols of olive oil was similar.
authors.affiliation: NMR Laboratory, Department of Chemistry, University of Crete, Voutes Campus, P.O. Box 2208, 71003 Heraklion, Crete, Greece.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Hatzakis
authors.forename: Anastasios
authors.initials: A
authors.lastname: Koidis
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Boskou
authors.forename: Photis
authors.initials: P
authors.lastname: Dais
mesh_terms: D008246:Lysophospholipids / Q000032:analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D000069463:Olive Oil; D010712:Phosphatidic Acids / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D010743:Phospholipids / Q000032:analysis*; D010938:Plant Oils / Q000737:chemistry*
chemical_list: D008246:Lysophospholipids; D000069463:Olive Oil; D010712:Phosphatidic Acids; D010716:Phosphatidylinositols; D010743:Phospholipids; D010938:Plant Oils; C032881:lysophosphatidic acid