title: [Phylogenetic analysis of human/swine/avian gene reassortant H1N2 influenza A virus isolated from a pig in China].
abstract: OBJECTIVE
  Our aim in this study was to determine the genetic characterization and probable origin of the H1N2 swine influenza virus (A/Swine/Guangxi/13/2006) (Sw/GX/13/06) from lung tissue of a pig in Guangxi province, China.
  
  
  METHODS
  Eight genes of Sw/GX/13/06 were cloned and genetically analyzed.
  
  
  RESULTS
  The hemagglutinin (HA), nucleoprotein (NP), matrix (M) and non-structural (NS) genes of Sw/GX/13/06 were most closely related to genes from the classical swine H1N1 influenza virus lineage. The neuraminidase (NA) and PB1 genes were most closely related to the corresponding genes from the human influenza H3N2 virus lineage. The remaining two genes PA and PB2 polymerase genes were most closely related to the genes from avian influenza virus lineage.
  
  
  CONCLUSIONS
  Phylogenetic analyses revealed that Sw/GX/13/06 was a human/swine/avian H1N2 virus, and closely related to H1N2 viruses isolated from pigs in United States (1999-2001) and Korea (2002). To our knowledge, Sw/GX/13/06 was the first triple-reassortant H1N2 influenza A virus isolated from a pig in China. Whether the Sw/GX/13/06 has a potential threat to breeding farm and human health remains to be further investigated.
authors.affiliation: Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, China. y.x.chen@hotmail.com
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Chen
authors.forename: Xueqiong
authors.initials: X
authors.lastname: Meng
authors.forename: Qi
authors.initials: Q
authors.lastname: Liu
authors.forename: Xia
authors.initials: X
authors.lastname: Huang
authors.forename: Shengbin
authors.initials: S
authors.lastname: Huang
authors.forename: Cuiquan
authors.initials: C
authors.lastname: Liu
authors.forename: Kaichuang
authors.initials: K
authors.lastname: Shi
authors.forename: Jiangang
authors.initials: J
authors.lastname: Guo
authors.forename: Fangfang
authors.initials: F
authors.lastname: Chen
authors.forename: Liping
authors.initials: L
authors.lastname: Hu
mesh_terms: D000818:Animals; D001717:Birds / Q000821:virology*; D002681:China; D005814:Genes, Viral; D005838:Genotype; D006801:Humans; D009980:Influenza A virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D008168:Lung / Q000166:cytology / Q000821:virology; D010802:Phylogeny; D011200:Poultry / Q000821:virology; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D012689:Sequence Homology, Nucleic Acid; D013552:Swine / Q000821:virology*