Triterpenes from Cynomorium songaricium--analysis of HCV protease inhibitory activity, quantification, and content change under the influence of heating.
Inhibitory activity of the three major triterpenes from the stems of Cynomorium songaricum--ursolic acid, acetyl ursolic acid, and malonyl ursolic acid hemiester--and their related compounds were tested for their inhibitory activity on HCV protease; malonyl ursolic acid hemiester was the most potent. A HPLC-PAD (photo diode array detector)-MS method was established to quantify the contents of each triterpene in C. songaricum. Using this method, the effect heating had on the contents was also investigated. It was found that among the three triterpenes, the content of malonyl ursolic acid hemiester decreased most quickly during the heating process.
Ma
Chao-Mei
CM
Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan.
Wei
Ying
Y
Wang
Zhi-Gang
ZG
Hattori
Masao
M
D002851:Chromatography, High Pressure Liquid; D031482:Cynomorium / Q000737:chemistry*; D004789:Enzyme Activation / Q000187:drug effects; D016174:Hepacivirus / Q000201:enzymology*; D013058:Mass Spectrometry; D015394:Molecular Structure; D010447:Peptide Hydrolases / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D014764:Viral Proteins / Q000378:metabolism; D000097245:Ursolic Acid
D010447:Peptide Hydrolases; D010936:Plant Extracts; D014315:Triterpenes; D014764:Viral Proteins; D000097245:Ursolic Acid