Immunogenicity and tolerance following HIV-1/HBV plant-based oral vaccine administration.
Transgenic tobacco plants expressing a HIV-1 polyepitope associated with hepatitis B (HBV) virus-like particles (VLPs) were previously described. It is demonstrated here that oral administration of these transgenic plants to humanized HSB mice to boost DNA-priming can elicit anti-HIV-1 specific CD8+ T cell activation detectable in mesenteric lymph nodes. Nevertheless, a significant regulatory T cell activation was induced in vivo by the vaccination protocols. The balance between tolerance and immunogenicity remains the main concern in the proof of concept of plant-based vaccine.
Guetard
Denise
D
Department of Virology, Unité de Rétrovirologie Moléculaire, CNRS URA 3015, Institut Pasteur, 28 rue du Dr Roux, 75015 Paris, France.
Greco
Raffaella
R
Cervantes Gonzalez
Minerva
M
Celli
Susanna
S
Kostrzak
Anna
A
Langlade-Demoyen
Pierre
P
Sala
Francesco
F
Wain-Hobson
Simon
S
Sala
Monica
M
D016915:AIDS Vaccines / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology*; D000284:Administration, Oral; D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D005434:Flow Cytometry; D015497:HIV-1 / Q000235:genetics; D017325:Hepatitis B Vaccines / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology*; D006515:Hepatitis B virus / Q000235:genetics; D007117:Immunization, Secondary / Q000379:methods; D008198:Lymph Nodes / Q000276:immunology; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets / Q000276:immunology; D051379:Mice; D030821:Plants, Genetically Modified; D050378:T-Lymphocytes, Regulatory / Q000276:immunology; D014026:Nicotiana; D019444:Vaccines, DNA / Q000276:immunology; D022462:Vaccines, Edible / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D022721:Vaccines, Virosome / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D014764:Viral Proteins / Q000096:biosynthesis / Q000235:genetics
D016915:AIDS Vaccines; D000939:Epitopes; D017325:Hepatitis B Vaccines; D019444:Vaccines, DNA; D022462:Vaccines, Edible; D022721:Vaccines, Virosome; D014764:Viral Proteins