title: Affinity precipitation of proteins by polyligands.
abstract: A novel technique for affinity precipitation has been developed in which multimeric target proteins are precipitated as a result of network formation by polymer-conjugated ligands (polyligands). A polyligand precipitant for avidin was synthesized by conjugation of biotin to a polyacrylamide-based backbone. The effects of mixing conditions, ligand substitution frequency, and molecular weight on affinity precipitation were examined using the biotin-PAAm precipitant. Biotin was replaced by iminobiotin to study the effect of the ligand-protein dissociation constant o affinity precipitation. The iminobiotin-PAAm precipitant was also used to examine the reversibility of the precipitation and recovery of the target protein after precipitation.
authors.affiliation: Biological Process Technology Institute, University of Minnesota, St Paul, Minnesota 55108, USA.
authors.forename: J E
authors.initials: JE
authors.lastname: Morris
authors.forename: A S
authors.initials: AS
authors.lastname: Hoffman
authors.forename: R R
authors.initials: RR
authors.lastname: Fisher