title: [Real-time polymerase chain reaction (RT-PCR)].
abstract: The work present PCR (Polymerase Chain Reaction) with the real time analysis of the increase of product allowing quick detection, duplication and identification of even slight amounts of nucleic acids. Essential methods used in molecular diagnostics, based on marked probes and fluorescence phenomenon were discussed. Increased sensitivity of technology, the possibility of analysis and visualization of products during the time of reaction, enabled the progress of highly effective diagnostics applications.
authors.affiliation: Zakład Biochemii Katedry Biochemii i Chemii Medycznej Pomorskiej Akademii Medycznej w Szczecinie, Szczecin.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Wiedro
authors.forename: Ewa
authors.initials: E
authors.lastname: Stachowska
authors.forename: Dariusz
authors.initials: D
authors.lastname: Chlubek
mesh_terms: D000818:Animals; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D006868:Hydrolysis; D025202:Molecular Diagnostic Techniques; D015336:Molecular Probe Techniques; D015341:Nucleic Acid Probes; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity
chemical_list: D005456:Fluorescent Dyes; D015341:Nucleic Acid Probes