Cholinesterase inhibitory and anti-amnesic activity of alkaloids from Corydalis turtschaninovii.
In the course of screening plants used in Korean folk medicine as memory enhancers, a 70% ethanol extract of tuber from Corydalis turtschaninovii Besser (Papaveraceae) showed significant acetylcholinesterase (AChE) inhibitory activity. Repeated column chromatography led to the isolation of a new aporphine alkaloid, oxoglaucidaline (9), and a new protoberberine, pseudodehydrocorydaline (13) together with 14 known compounds (1-8, 10-12, and 14-16). The chemical structures of isolated compounds were elucidated base on extensive 1D and 2D NMR spectroscopic data. Compounds 1-16 were investigated in vitro for their anti-cholinesterase activity using the mice cortex AChE enzyme. In further study, the anti-amnesic activities of pseudoberberine (16) in mice on the learning and memory impairments induced by scopolamine (1.0 mg/kg, i.p.) were examined. This alkaloid (5.0 mg/kg, p.o.) administration significantly reversed cognitive impairments in mice by passive avoidance test (P<0.05). It also reduced escape latencies in training trials and prolonged swimming times in the target quadrant during the probe trial in the water maze task (P<0.05). These results indicated that Corydalis turtschaninovii due to its alkaloids have anti-cholinesterase activity and pseudoberberine and other alkaloids have anti-amnesic activities that may be useful for cognitive impairment treatment.
Hung
Tran Manh
TM
College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea.
Na
Minkyun
M
Dat
Nguyen Tien
NT
Ngoc
Tran Minh
TM
Youn
Uijoung
U
Kim
Hong Jin
HJ
Min
Byung-Sun
BS
Lee
Jongpill
J
Bae
Kihwan
K
D000110:Acetylcholinesterase / Q000187:drug effects / Q000378:metabolism; D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D000647:Amnesia / Q000188:drug therapy; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D001599:Berberine / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000494:pharmacology; D001600:Berberine Alkaloids / Q000302:isolation & purification / Q000494:pharmacology*; D002540:Cerebral Cortex / Q000378:metabolism; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D003072:Cognition Disorders / Q000188:drug therapy*; D031311:Corydalis / Q000737:chemistry*; D007723:Korea; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D018782:Maze Learning / Q000187:drug effects; D008518:Medicine, East Asian Traditional; D051379:Mice; D008813:Mice, Inbred ICR; D035281:Plant Tubers; D051381:Rats; D017207:Rats, Sprague-Dawley
D000470:Alkaloids; D001600:Berberine Alkaloids; D002800:Cholinesterase Inhibitors; C578968:pseudoberberine; D001599:Berberine; D000110:Acetylcholinesterase