title: [Theoretical and experimental studies on solubility parameters of multiple components for traditional Chinese materia medica with HPLC].
abstract: OBJECTIVE
  To establish the theories and methods to determine solubility parameters of multiple components for the traditional Chinese material medica (TCMM) with HPLC.
  
  
  METHODS
  The mathematical expresses to determin the solubility parameters were established according to chromatographic and Hildebrand-Scatchard theories, The HPLC experiments were carried out at 40 degrees C on an Alltech Apollo C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetone and water in gradient mode. The flow rate was 1.0 mL min(-1), and the detection wavelength was 254 nm. The injection volume was 1 0 microL.
  
  
  RESULTS
  The mathematical expresses between the retention time and the solubility parameters were established and used to determin caffeine solubility parameter as 28.31 J(1/2) cm(-3/2) in accordance with 28.84 J(1/2) cm(-3/2) reported by literature, and those of aloe-emodin, rhein, emodin, physcione as 39.70 J(1/2) cm(-3/2), 39.08 J(1/2) cm(-3/2), 38.37 J(1/2) cm(-3/2), 36.42 J(1/2) cm(-3/2) respectively.
  
  
  CONCLUSIONS
  The retention time of HPLC can be used to determine the solubility parameters of multiple componets. The established method is useful for the compatibility rule study of traditional Chinese medicine.
authors.affiliation: Pharmacogenetic Research Institute, Central South University, Changsha 410078, China. pharmsharking@tom.com
authors.forename: Fu-Yuan
authors.initials: FY
authors.lastname: He
authors.forename: Hong-Hao
authors.initials: HH
authors.lastname: Zhou
authors.forename: Jie-Ying
authors.initials: JY
authors.lastname: Luo
authors.forename: Kai-Wen
authors.initials: KW
authors.lastname: Deng
authors.forename: Jin
authors.initials: J
authors.lastname: Fu
authors.forename: Feng-Juan
authors.initials: FJ
authors.lastname: Pi
authors.forename: De-Zhi
authors.initials: DZ
authors.lastname: Wu
authors.forename: Ru-Wen
authors.initials: RW
authors.lastname: Dai
mesh_terms: D000880:Anthraquinones / Q000737:chemistry; D002110:Caffeine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004642:Emodin / Q000031:analogs & derivatives / Q000737:chemistry; D008419:Materia Medica / Q000737:chemistry*; D008956:Models, Chemical; D012044:Regression Analysis; D012995:Solubility; D013696:Temperature
chemical_list: D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D002110:Caffeine; C008905:physcione; D004642:Emodin; C020491:rhein