title: Neem leaf glycoprotein helps to generate carcinoembryonic antigen specific anti-tumor immune responses utilizing macrophage-mediated antigen presentation.
abstract: In an objective to generate effective carcinoembryonic antigen (CEA) specific anti-tumor immune response in Swiss mice, CEA was presented using macrophages with adjuvant help from neem leaf glycoprotein (NLGP). Such vaccination generates significantly higher antibody (IgG2a) and T cell response than immunization protocol without NLGP. NLGP controls the function of both B cells and macrophages by altering the expressions of various regulatory molecules, like, CD19, CD11b, etc. NLGP also directs CEA vaccination towards Th1 bias, by modulating cytokine secretion. This NLGP-generated anti-CEA immune response would be effective as a vaccine to lyse CEA(+) tumors in vitro and in vivo.
authors.affiliation: Department of Immunoregulation and Immunodiagnostics, Chittaranjan National Cancer Institute, 37, S.P. Mukherjee Road, Kolkata 700026, West Bengal, India.
authors.forename: Koustav
authors.initials: K
authors.lastname: Sarkar
authors.forename: Anamika
authors.initials: A
authors.lastname: Bose
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Chakraborty
authors.forename: Enamul
authors.initials: E
authors.lastname: Haque
authors.forename: Diptendu
authors.initials: D
authors.lastname: Ghosh
authors.forename: Shyamal
authors.initials: S
authors.lastname: Goswami
authors.forename: Tathagata
authors.initials: T
authors.lastname: Chakraborty
authors.forename: Subrata
authors.initials: S
authors.lastname: Laskar
authors.forename: Rathindranath
authors.initials: R
authors.lastname: Baral
mesh_terms: D000276:Adjuvants, Immunologic; D000818:Animals; D000912:Antibodies, Neoplasm / Q000097:blood; D017951:Antigen Presentation; D031601:Azadirachta / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D045744:Cell Line, Tumor; D002470:Cell Survival; D003601:Cytotoxicity Tests, Immunologic; D003602:Cytotoxicity, Immunologic; D005260:Female; D006023:Glycoproteins / Q000276:immunology*; D008264:Macrophages / Q000276:immunology*; D051379:Mice; D009369:Neoplasms / Q000276:immunology* / Q000473:pathology; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000276:immunology*; D016019:Survival Analysis; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D000912:Antibodies, Neoplasm; D002272:Carcinoembryonic Antigen; D006023:Glycoproteins; D010940:Plant Proteins