[Utility of in-house PCR for HLA-B27 typing: comparison of concordance rate between PCR kit and in-house PCR].
BACKGROUND
Commercial kits of PCR method are widely used in HLA-B27 typing; however, their cost is relatively high. In this study, we evaluated the utility of an in-house PCR method by comparing it with that of a commercial kit.


METHODS
HLA-B27 typing was done in 188 patients by using two PCR methods, Absolute HLAB27 PCR kit (Biosewoom, Korea) and an in-house PCR method. The primers used in the in-house method were prepared by Bioneer (Korea). Both PCR tests were done by Gene Amp PCR System 9600 (Perkin-Elmer Centus Corp., USA).


RESULTS
The commercial kit and in-house PCR showed 100% concordance rate with each other in HLA-B27 typing. Of 188 patients tested 72 (38.3%) were positive and 116 (61.7%) were negative by the both tests. Of 62 patients with ankylosing spondylitis, 50 were positive (80.7%).


CONCLUSIONS
The in-house PCR is a reliable and cost-effective method and can replace or supplement commercial kits for HLA-B27 typing.
Cho
Sun Young
SY
Department of Laboratory Medicine, Kyung-Hee Medical Center, School of Medicine, Kyoung-Hee University, Seoul, Korea.
Lee
Kwang Gil
KG
Park
Su Yon
SY
Lee
Hee Joo
HJ
D000328:Adult; D005260:Female; D015796:HLA-B27 Antigen / Q000097:blood / Q000235:genetics*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D015796:HLA-B27 Antigen; D011933:Reagent Kits, Diagnostic