[Effects of curcumin on secretion of adiponectin and interleukin-6 in human adipose tissues: an in vitro study].
OBJECTIVE
To investigate the effects of different concentrations of curcumin on secretion of adiponectin (APN) and interleukin-6 (IL-6) in human adipose tissues cultivated in vitro.


METHODS
Seven male patients with kidney stones were admitted. Abdominal subcutaneous adipose tissue and perirenal adipose tissue were collected from the operating-patients, and were cultivated with different concentrations of curcumin (10 and 100 microg/ml) in vitro. The contents of APN and IL-6 in the culture medium of adipose tissue cells were measured by enzyme-linked immunosorbent assay (ELISA) after they were cultured for 6 and 24 hours.


RESULTS
Compared with the blank control group, the content of APN in the adipose tissue culture medium was increased by 100 microg/ml curcumin (P<0.05) after 6-hour culture, and the content of IL-6 was significantly decreased by 100 microg/ml curcumin after 6- and 24-hour culture (P<0.05).


CONCLUSIONS
100 microg/ml curcumin can increase APN secretion and decrease IL-6 secretion in human adipose tissues cultivated in vitro.
Qu
Xiao-bing
XB
Department of Geriatrics, the Second Xiangya Hospital, Central South University, Changsha, Hunan Province 410011, China. quxiaobing1@163.com
Zhao
Shui-ping
SP
Xu
Jing
J
Dong
Li-ni
LN
D052242:Adiponectin / Q000378:metabolism*; D000273:Adipose Tissue / Q000378:metabolism*; D000328:Adult; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D046509:Tissue Culture Techniques
D052242:Adiponectin; D015850:Interleukin-6; D003474:Curcumin