A proteomic analysis of rice seedlings responding to 1,2,4-trichlorobenzene stress.
The proteomic analysis of rice (Oryza sativa L.) roots and leaves responding to 1,2,4-trichlorobenzene (TCB) stress was carried out by two dimensional gel electrophoresis, mass spectrometric (MS), and protein database analysis. The results showed that 5 mg/L TCB stress had a significant effect on global proteome in rice roots and leaves. The analysis of the category and function of TCB stress inducible proteins showed that different kinds of responses were produced in rice roots and leaves, when rice seedlings were exposed to 5 mg/L TCB stress. Most responses are essential for rice defending the damage of TCB stress. These responses include detoxication of toxic substances, expression of pathogenesis-related proteins, synthesis of cell wall substances and secondary compounds, regulation of protein and amino acid metabolism, activation of methionine salvage pathway, and also include osmotic regulation and phytohormone metabolism. Comparing the TCB stress inducible proteins between the two cultivars, the beta-glucosidase and pathogenesis-related protein family 10 proteins were particularly induced by TCB stress in the roots of rice cultivar (Oryza sativa L.) Aizaizhan, and the glutathione S-transferase and aci-reductone dioxygenase 4 were induced in the roots of rice cultivar Shanyou 63. This may be one of the important mechanisms for Shanyou 63 having higher tolerance to TCB stress than Aizaizhan.
Ge
Cailin
C
Jiangsu Provencial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, China. Gecailin10@163.com
Wan
Dingzhen
D
Wang
Zegang
Z
Ding
Yan
Y
Wang
Yulong
Y
Shang
Qi
Q
Ma
Fai
F
Luo
Shishi
S
D002722:Chlorobenzenes / Q000633:toxicity; D004591:Electrophoresis, Polyacrylamide Gel; D007525:Isoelectric Focusing; D012275:Oryza / Q000187:drug effects / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000032:analysis*; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002722:Chlorobenzenes; D010940:Plant Proteins; C009947:1,2,4-trichlorobenzene