The sensitization of glioma cells to cisplatin and tamoxifen by the use of catechin.
Telomerase expression strongly correlates with the grade of malignancy in glioma with inhibition illustrating a definite increase in chemosensitivity. This study was designed to investigate the effects of a green tea derivative, epigallocatechin-3-gallate (EGCG); together with either cisplatin or tamoxifen in glioma, and to investigate whether these effects are mediated through telomerase suppression. EGCG showed a significant cytotoxic effect on 1321N1 cells after 24 h and on U87-MG cells after 72 h (P < 0.001) without significantly affecting the normal astrocytes. Treatment with EGCG inhibited telomerase expression significantly (P < 0.01) and enhanced the effect of cisplatin and tamoxifen in both 1321N1 (P < 0.01) and U87-MG (P < 0.001) cells. EGCG, as a natural product has enormous potential to be an anti-cancer agent capable of enhancing tumour cell sensitivity to therapy.
Shervington
Amal
A
Brain Tumour North West, Faculty of Science, University of Central Lancashire, Preston, UK. aashervington@uclan.ac.uk
Pawar
Vidya
V
Menon
Sharad
S
Thakkar
Dipti
D
Patel
Rahima
R
D000970:Antineoplastic Agents / Q000494:pharmacology*; D002392:Catechin / Q000031:analogs & derivatives / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology*; D004354:Drug Screening Assays, Antitumor; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D005910:Glioma / Q000473:pathology*; D006801:Humans; D013629:Tamoxifen / Q000494:pharmacology*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism
D000970:Antineoplastic Agents; D013629:Tamoxifen; D002392:Catechin; C045651:epigallocatechin gallate; D019098:Telomerase; D002945:Cisplatin