Experimental investigation of G6P production and simultaneous ATP regeneration by conjugated enzymes in an ultrafiltration hollow-fiber reactor.
Enzymatic synthesis of glucose 6-phosphate from glucose and ATP catalyzed by glucokinase from B. stearothermophilus and enzymatic regeneration of ATP from ADP and acetyl phosphate catalyzed by acetatekinase from B. stearothermophilus were simultaneously performed in an Ultrafiltration hollow-fiber reactor of the multitubular heat-exchanger type. Experimental results of space-time yield, the conversion, and ATP recycle number were in good agreement with the theoretical predictions based on the simple analytical model developed in the preceding article. The best results for space-time yield and conversion were Y(s) = 1.97 mol/m(3) h and X = 92.8%, respectively, under the same conditions, and the best result for the ATP recycle number was N(R) = 2130 under conditions different from those above. However, Y(s) = 1.72 mol/m(3) h, X = 81.2%, and N(R) = 1620 were the results when the space-time yield, conversion, and recycle number were at the highest in combination under the same conditions. Results of long-term operation showed that the apparent remaining activity of the enzyme system was ca. 55% after continuous operation for 16 days, the decrease in the enzyme activity being faster than that expected from their half-life times determined individually in the homogeneous system.
Ishikawa
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Department of Chemical Engineering, University of Osaka Prefecture, 804 Mozu-Umemachi 4-cho, Sakai, Osaka 591, Japan.
Takase
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Tanaka
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Hikita
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