14-3-3 regulates the G2/M transition in the basidiomycete Ustilago maydis.
14-3-3 proteins are a family of highly conserved polypeptides that function as small adaptors that facilitate a diverse array of cellular processes by binding phosphorylated target proteins. One of these processes is the regulation of the cell cycle. Here we characterized the role of Bmh1, a 14-3-3 protein, in the cell cycle regulation of the fungus Ustilago maydis. We found that this protein is essential in U. maydis and that it has roles during the G2/M transition in this organism. The function of 14-3-3 in U. maydis seems to mirror the proposed role for this protein during Schizosaccharomyces pombe cell cycle regulation. We provided evidence that in U. maydis 14-3-3 protein binds to the mitotic regulator Cdc25. Comparison of the roles of 14-3-3 during cell cycle regulation in other fungal system let us to discuss the connections between morphogenesis, cell cycle regulation and the evolutionary role of 14-3-3 proteins in fungi.
Mielnichuk
Natalia
N
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología-CSIC, DARWIN 3, Campus de Cantoblanco-UAM, 28049 Madrid, Spain.
Pérez-Martín
José
J
D048948:14-3-3 Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D000595:Amino Acid Sequence; D015152:Blotting, Northern; D015153:Blotting, Western; D002453:Cell Cycle / Q000235:genetics / Q000502:physiology*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016195:G2 Phase / Q000235:genetics / Q000502:physiology; D015966:Gene Expression Regulation, Fungal; D047468:Immunoprecipitation; D008938:Mitosis / Q000235:genetics / Q000502:physiology; D008969:Molecular Sequence Data; D009154:Mutation; D011485:Protein Binding; D017386:Sequence Homology, Amino Acid; D014588:Ustilago / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D020687:cdc25 Phosphatases / Q000235:genetics / Q000378:metabolism
D048948:14-3-3 Proteins; D005656:Fungal Proteins; D020687:cdc25 Phosphatases