title: Arf family GTP loading is activated by, and generates, positive membrane curvature.
abstract: Small G-proteins belonging to the Arf (ADP-ribosylation factor) family serve as regulatory proteins for numerous cellular processes through GTP-dependent recruitment of effector molecules. In the present study we demonstrate that proteins in this family regulate, and are regulated by, membrane curvature. Arf1 and Arf6 were shown to load GTP in a membrane-curvature-dependent manner and stabilize, or further facilitate, changes in membrane curvature through the insertion of an amphipathic helix.
authors.affiliation: MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 0QH, UK. richard.lundmark@medchem.umu.se
authors.forename: Richard
authors.initials: R
authors.lastname: Lundmark
authors.forename: Gary J
authors.initials: GJ
authors.lastname: Doherty
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Vallis
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Peter
authors.forename: Harvey T
authors.initials: HT
authors.lastname: McMahon
mesh_terms: D020823:ADP-Ribosylation Factor 1 / Q000235:genetics / Q000378:metabolism*; D000089982:ADP-Ribosylation Factor 6; D020727:ADP-Ribosylation Factors / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure; D006153:Guanosine Diphosphate / Q000378:metabolism; D006160:Guanosine Triphosphate / Q000378:metabolism*; D006801:Humans; D008081:Liposomes / Q000737:chemistry / Q000378:metabolism; D008854:Microscopy, Electron; D008969:Molecular Sequence Data; D017386:Sequence Homology, Amino Acid; D018411:Spodoptera
chemical_list: D000089982:ADP-Ribosylation Factor 6; D008081:Liposomes; D006153:Guanosine Diphosphate; D006160:Guanosine Triphosphate; D020823:ADP-Ribosylation Factor 1; D020727:ADP-Ribosylation Factors; C000715348:ARF6 protein, human