Cloning and molecular characterization of a novel x-type glutenin gene Glu-D(t)1 from Aegilops tauschii.
A novel gene encoding an x-type high molecular weight glutenin subunit (HMW-GS), designated 1Dx1.1(t), was isolated from Aegilops tauschii. It is the largest HMW-GS gene reported so far in this species and its product has a slower mobility than that of subunit 1Ax1 in SDS-PAGE. The open reading frame (ORF) of the gene was 2,628 bp, encoding a protein of 874 amino acid residues. Comparisons of amino acid sequences showed that subunit 1Dx1.1(t) had high similarity with other 1Dx subunits but also had two unique characteristics. Firstly, a tripeptide of consensus LQE present in the N-terminal domains of other 1Dx subunits was absent from subunit Dx1.1(t). Secondly, three copies of tandem duplications of the tripeptide motif GQQ and a novel tripeptide sequence (GQL) were present in its central repetitive domain. Phylogenetic analysis showed that subunit 1Dx1.1(t) clustered with other known 1Dx subunits.
Fang
Jingye
J
China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Key Laboratory of Molecular Biophysics, Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, Hubei, PR China.
Liu
Yong
Y
Zhang
Change
C
Yang
Guangxiao
G
Li
Kexiu
K
Shewry
Peter R
PR
He
Guangyuan
G
D000483:Alleles; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D019143:Evolution, Molecular; D018506:Gene Expression Regulation, Plant; D005983:Glutens / Q000032:analysis / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016366:Open Reading Frames / Q000235:genetics; D010802:Phylogeny; D006109:Poaceae / Q000235:genetics*; D021122:Protein Subunits / Q000737:chemistry / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D021122:Protein Subunits; D005983:Glutens; C037302:glutenin