title: Analysis of the transcriptional regulation of YlODC gene from the dimorphic fungus Yarrowia lipolytica.
abstract: Polyamines are small polycations essential for cell growth. Ornithine decarboxylase (ODC), the first enzyme of the polyamine synthesis pathway, is one of the most regulated enzymes of eukaryotes. In the present report, the transcriptional regulation of the gene encoding ODC from the dimorphic fungus Yarrowia lipolytica (YlODC) was analyzed. To this end we made a transcriptional fusion of its promoter with the ORF of the beta-glucuronidase gene from Escherichia coli, and analyzed the effect of polyamines and the dimorphic transition on the rate of transcription. The results obtained show that there exists a weak but reproducible transcriptional regulation of YlODC under the analyzed conditions. Our data suggest that gene transcription is down-regulated by putrescine, and stimulated by spermidine. It is suggested that the simultaneous operation of transcriptional and post-translational regulatory mechanisms endows the cell with a more efficient mechanism to control the physiological processes that depend on polyamines.
authors.affiliation: División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa de San José 2055, Apartado Postal 3-74 Tangamanga, 78210 San Luis Potosí, Slp., México. jbremont@ipicyt.edu.mx
authors.forename: Juan Francisco
authors.initials: JF
authors.lastname: Jiménez-Bremont
authors.forename: José
authors.initials: J
authors.lastname: Ruiz-Herrera
mesh_terms: D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics / Q000378:metabolism; D009955:Ornithine Decarboxylase / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D011700:Putrescine / Q000494:pharmacology*; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D013095:Spermidine / Q000494:pharmacology*; D014158:Transcription, Genetic / Q000187:drug effects; D025062:Yarrowia / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development
chemical_list: D005656:Fungal Proteins; D011993:Recombinant Fusion Proteins; D005966:Glucuronidase; D009955:Ornithine Decarboxylase; D013095:Spermidine; D011700:Putrescine