Determining the pharmacological activity of Physalis peruviana fruit juice on rabbit eyes and fibroblast primary cultures.
OBJECTIVE
The pharmacologic activity of compounds isolated from Physalis peruviana has been demonstrated. The use of this fruit juice for treating pterygium has been reported in Colombian traditional medicine. However, studies demonstrating the fruit juice's pharmacologic activity when used in this disease have not been published to date. In the present study the anti-inflammatory and cytostatic activities of P. peruviana fruit juice in a rabbit eye inflammatory model were investigated.


METHODS
A novel rabbit eye inflammation model was developed for studying the juice's anti-inflammatory activity (based on an adaptation of the Draize test). Cytostatic activity was evaluated by measuring and comparing growth rates of cultured fibroblasts exposed and not exposed to various fruit juice concentrations.


RESULTS
P. peruviana fruit juice exhibited a mild anti-inflammatory activity compared with methylprednisolone, a known anti-inflammatory drug. An interesting dose-dependent cytostatic effect on cultured fibroblasts was also established.


CONCLUSIONS
The data found suggest that the P. peruviana fruit juice anti-pterygium effect described in traditional medicine may be related to its inhibiting fibroblast growth. The present study contributes to the pharmacologic knowledge regarding a remedy commonly used in Colombian traditional medicine.
Pardo
Juan Manuel
JM
Facultad de Medicina, Universidad Nacional de Colombia, Ciudad Universitaria, Bogotá, Colombia. jmpardom@unal.edu.co
Fontanilla
Marta Raquel
MR
Ospina
Luis Fernando
LF
Espinosa
Lady
L
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000494:pharmacology*; D002455:Cell Division / Q000187:drug effects; D002478:Cells, Cultured; D003105:Colombia; D054697:Cytostatic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D009877:Endophthalmitis / Q000473:pathology*; D005123:Eye / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects*; D008297:Male; D008519:Medicine, Traditional; D008775:Methylprednisolone / Q000494:pharmacology; D032321:Physalis; D028321:Plant Preparations / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D011625:Pterygium / Q000188:drug therapy / Q000473:pathology; D011817:Rabbits
D000893:Anti-Inflammatory Agents; D054697:Cytostatic Agents; D028321:Plant Preparations; D008775:Methylprednisolone