[Several carotenoids of vegetable determinated by high performance liquid chromatography].
OBJECTIVE
To detect alpha-carotene (alpha-C), beta-carotene (beta-C), cryptoxanthin (Cryp), lutein (Lut) and lycopene (Lyco) levels in vegetables.


METHODS
A HPLC method with C30 column was used to detect several carotenoids at the wave length of 450 nm. A gradient elution method with methanol, methyl-t-butyl-ether and ammonium acetate solution was used to separate the carotenoids.


RESULTS
The level of Lut, Cryp, alpha-c, beta-c and lycopene were linearly related with the peak area. The recoveries of the standard were between 94.7%-108.1%. The relative standard deviations were less than 4.62%.


CONCLUSIONS
The method could be simple and credible. It could separate 5 carotenoids in vegetables simultaneously.
Zhao
Xianfeng
X
Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Pan
Lili
L
Meng
Jing
J
Wang
Yin
Y
et al
D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D065366:Cryptoxanthins; D014675:Vegetables / Q000737:chemistry*; D024341:Xanthophylls / Q000032:analysis; D019207:beta Carotene / Q000032:analysis
D065366:Cryptoxanthins; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; C041635:alpha-carotene