[Screening peptides binding specifically to large intestinal cancer LoVo cells from phage random peptide library].
OBJECTIVE
To screen the polypeptides specifically binding to human large intestinal cancer LoVo cells from a phage-displayed peptide library for potential use as targeting vectors for large intestinal cancer therapy.


METHODS
With the LoVo cells as the target cells and human normal large intestinal mucosal epithelial cells as the absorber cells for subtraction biopanning from a c7c phage-display peptide library, the positive phage clones were identified by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence detection. The amino acid sequences of the identified peptides were deduced by DNA sequencing.


RESULTS
After 3 rounds of screening, 5 positive phage clones showing specific binding to LoVo cells and containing conserved motif RPMP were obtained from the 20 randomly selected clones.


CONCLUSIONS
Specific peptide against large intestinal cancer cells can be obtained from a phage-display peptide library for use as potential vectors for targeting therapy of large intestinal cancer.
Liao
Kang-Xiong
KX
Department of General Surgery, Nanfang Hosptial, Southern Medical University, Guangzhou 510515, China.
Yao
Xue-Qing
XQ
Wu
Cheng-Tang
CT
Lin
Feng
F
Wu
Wu-Lin
WL
Zeng
Sui-de
SD
Luo
Yu-Qi
YQ
Lei
Shang-Tong
ST
D000595:Amino Acid Sequence; D001483:Base Sequence; D001667:Binding, Competitive; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D006801:Humans; D008969:Molecular Sequence Data; D019151:Peptide Library; D010455:Peptides / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D011485:Protein Binding
D019151:Peptide Library; D010455:Peptides