Enzymatic ligation creates discrete multinanoparticle building blocks for self-assembly.
Enzymatic ligation of discrete nanoparticle-DNA conjugates creates nanoparticle dimer and trimer structures in which the nanoparticles are linked by single-stranded DNA, rather than by double-stranded DNA as in previous experiments. Ligation was verified by agarose gel and small-angle X-ray scattering. This capability was utilized in two ways: first, to create a new class of multiparticle building blocks for nanoscale self-assembly and, second, to develop a system that can amplify a population of discrete nanoparticle assemblies.
Claridge
Shelley A
SA
Department of Chemistry, University of California, Berkeley, California 94720-1460, USA.
Mastroianni
Alexander J
AJ
Au
Yeung B
YB
Liang
Huiyang W
HW
Micheel
Christine M
CM
Fréchet
Jean M J
JM
Alivisatos
A Paul
AP
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002852:Chromatography, Ion Exchange / Q000379:methods; D018736:DNA Adducts / Q000138:chemical synthesis* / Q000737:chemistry; D011088:DNA Ligases / Q000737:chemistry*; D004277:DNA, Single-Stranded; D004591:Electrophoresis, Polyacrylamide Gel; D006046:Gold / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
D018736:DNA Adducts; D004277:DNA, Single-Stranded; D006046:Gold; D011088:DNA Ligases