[Assessment of therapeutic effect of losartan on diabetes mellitus with gas chromatography-based metabonomics].
OBJECTIVE
To assess the therapeutic effect of losartan on type 2 diabetes mellitus (DM2) with gas chromatography (GC)-based metabonomics.


METHODS
DM2 patients were dosed with losartan (100 mg/d) and urines were collected at week 8 and 12. The biochemical criteria (blood pressure, urinary albumen, urinary 8-hydroxy-2'-deoxyguanosine and blood creatinine) were analyzed. Urine samples were derivatived and analyzed by GC. Multivariate metabonomics analysis was performed after peak alignment.


RESULTS
After 8-12 weeks, losartan showed little curative effect and no remarked changes of biochemical criteria were observed. However, metabonomics analysis revealed that some biomarkers such as glucitol and inositol changed.


CONCLUSIONS
GC-based metabonomics analysis enables the rapid identification of metabolic differences and provides information concerning therapeutic effect of losartan.
Yuan
Kai-Long
KL
Metabonomics Research Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
Shi
Xian-Zhe
XZ
Lu
Xin
X
Gao
Peng
P
Xu
Guo-Wang
GW
D000080242:8-Hydroxy-2'-Deoxyguanosine; D000419:Albuminuria / Q000652:urine; D015415:Biomarkers / Q000097:blood / Q000737:chemistry / Q000652:urine; D002849:Chromatography, Gas / Q000379:methods*; D003404:Creatinine / Q000097:blood; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000652:urine; D003920:Diabetes Mellitus / Q000188:drug therapy*; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy*; D016903:Drug Monitoring; D006801:Humans; D007004:Hypoglycemic Agents / Q000627:therapeutic use*; D007294:Inositol / Q000737:chemistry; D019808:Losartan / Q000627:therapeutic use*; D055442:Metabolome / Q000187:drug effects; D013012:Sorbitol / Q000737:chemistry
D015415:Biomarkers; D007004:Hypoglycemic Agents; D007294:Inositol; D013012:Sorbitol; D000080242:8-Hydroxy-2'-Deoxyguanosine; D003404:Creatinine; D003849:Deoxyguanosine; D019808:Losartan