Determination of dimethylarginine levels in rats using HILIC-MS/MS: an in vivo microdialysis study.
Nitric oxide (NO) is one of the most important mediators and neurotransmitters and its levels change under pathological conditions. NO production may be regulated by endogenous nitric oxide synthase (NOS) inhibitors, in particular asymmetric dimethylarginine (ADMA). Most of the interest is focused on ADMA, since this compound is present in plasma and urine and accumulation of ADMA has been described in many disease states but little is known about cerebrospinal fluid (CSF) concentrations of this compound and of its structural isomer symmetric dimethylarginine (SDMA). To determine the levels of methylarginines, we here present a new hydrophilic interaction chromatography (HILIC)-MS/MS method for the precise determination of these substances in CSF from microdialysis samples of rat prefrontal cortex (PFC). The method requires only minimal sample preparation and features isotope-labelled internal standards.
Zotti
Margherita
M
Department of Biomedical Sciences, University of Foggia, Foggia, Italy.
Schiavone
Stefania
S
Tricarico
Filomena
F
Colaianna
Marilena
M
D'Apolito
Oceania
O
Paglia
Giuseppe
G
Corso
Gaetano
G
Trabace
Luigia
L
D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid / Q000592:standards; D002853:Chromatography, Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000134:cerebrospinal fluid / Q000592:standards; D017551:Microdialysis / Q000379:methods*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D017397:Prefrontal Cortex / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*
D004791:Enzyme Inhibitors; C024917:symmetric dimethylarginine; C018524:N,N-dimethylarginine; D001120:Arginine; D019001:Nitric Oxide Synthase