Cytokine adsorption/release on uniform magnetic nanoparticles for localized drug delivery.
Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-gamma) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation, decomposition in organic media, and laser pyrolysis. To facilitate IFN-gamma adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-gamma and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-gamma adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases.
Mejías
Raquel
R
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Darwin 3, Campus de Cantoblanco, E-28049 Madrid, Spain.
Costo
Rocío
R
Roca
Alejandro G
AG
Arias
Cristina F
CF
Veintemillas-Verdaguer
Sabino
S
González-Carreño
Teresita
T
del Puerto Morales
María
M
Serna
Carlos J
CJ
Mañes
Santos
S
Barber
Domingo F
DF
D000327:Adsorption; D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D004337:Drug Carriers / Q000737:chemistry; D004339:Drug Compounding; D016503:Drug Delivery Systems / Q000379:methods*; D004355:Drug Stability; D006863:Hydrogen-Ion Concentration; D007371:Interferon-gamma / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D017737:Macrophages, Peritoneal / Q000187:drug effects / Q000276:immunology; D008280:Magnetics; D051379:Mice; D008810:Mice, Inbred C57BL; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000188:drug therapy / Q000276:immunology; D013499:Surface Properties; D014961:X-Ray Diffraction
D000970:Antineoplastic Agents; D004337:Drug Carriers; D007371:Interferon-gamma