[Detection of the new type of aminoglycoside acetyltransferase gene in Enterobacter cloacae isolates].
OBJECTIVE
To investigate the prevalence of aac(6')-Ib-cr gene in Enterobacter cloacae isolates.


METHODS
PCR and sequencing were performed on 81 strains of Enterobacter cloacae isolated clinically in Anhui province to identify aac (6')-IB-cr gene. Disk diffusion method was used to test the susceptibility of the Enterobacter cloacae isolates with aac (6')-Ib-cr gene to fluoroquinolones, aminoglycosides, and other antimicrobial agents. AmpC and extended-spectrum beta-lactamases (ESBLs) were detected by modified three-dimensional extract test, and the molecular typing was analyzed by ERIC-PCR.


RESULTS
Aac (6')-Ib-cr gene was identified in 3 (3.7%) of the 81 Enterobacter cloacae isolates with different ERIC-PCR patterns. The isolates with aac (6')-Ib-cr gene were resistant to fluoroquinolones, aminoglycosides, chloramphenicol, ampicillin, cefoxitin, and second and third generation cephalosporins. Two of the 3 isolates produced AmpC and ESBLs, and 1 isolate produced only ESBLs.


CONCLUSIONS
Aac (6')-Ib-cr gene is prevalent in Enterobacter cloacae isolates with resistance to most of antimicrobial agents and no clone spread in found in them.
Xiong
Zi-Zhong
ZZ
Department of Infectious Diseases, First Affiliated Hospital, Anhui Medical University, Hefei 230022, China.
Wang
Peng
P
Wang
Zhong-Xin
ZX
Li
Jun
J
D000123:Acetyltransferases / Q000235:genetics*; D000890:Anti-Infective Agents / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics*; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D016972:Enterobacter cloacae / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification; D005838:Genotype; D006801:Humans; D008826:Microbial Sensitivity Tests; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D000890:Anti-Infective Agents; D001426:Bacterial Proteins; D004269:DNA, Bacterial; D000123:Acetyltransferases; C019681:aminoglycoside acetyltransferase