Standardization of fluorescence measurements: criteria for the choice of suitable standards and approaches to fit-for-purpose calibration tools.
This report summarizes problems associated with the comparability of measurements of photoluminescence and procedures for the characterization of relevant instruments, focusing on physical and chemical fluorescence standards. To provide recommendations on selecting and using such standards, we derive general and scope-specific requirements and quality criteria for suitable devices and materials and briefly address metrological requirements linked to the realization of comparable measurements. Special emphasis is dedicated to liquid and solid chromophore-based fluorescence standards developed or currently tested by us.
Resch-Genger
Ute
U
Federal Institute for Materials Research and Testing (BAM), I.5 Bioanalytics, Richard-Willstaetter-Str. 11, 12489 Berlin-Adlershof, Germany. ute.resch@bam.de
Hoffmann
Katrin
K
Hoffmann
Angelika
A
D002138:Calibration; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000494:pharmacology; D008027:Light; D018613:Microscopy, Confocal / Q000295:instrumentation / Q000379:methods / Q000592:standards; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D010777:Photochemistry / Q000379:methods; D012015:Reference Standards; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000295:instrumentation / Q000379:methods* / Q000592:standards*
D005456:Fluorescent Dyes