title: Vaccination of broiler chickens with dispersed dry powder vaccines as an alternative for liquid spray and aerosol vaccination.
abstract: Vaccination of chickens with dispersable dry powder vaccines was compared with commercial liquid vaccines. A Clone 30 Newcastle disease vaccine virus was spray dried with mannitol or with a mixture of trehalose, polyvinylpyrrolidone and bovine serum albumin. A coarse (+/-30 microm) and fine (+/-7 microm) powder were produced with both formulations. A commercial reconstituted Clone 30 vaccine was applied as coarse liquid spray (+/-222 microm) or fine liquid aerosol (+/-24 microm). Reduction of virus concentration in the air after dispersion/nebulization was monitored by air sampling and was explained by sedimentation of coarse particles/droplets and evaporation of fine droplets. The vaccine formulations induced high haemagglutination inhibition antibody titres in the serum of 4-week-old broilers (2(7) at 4 weeks post-vaccination). The good serum antibody response with the fine liquid aerosol despite extensive inactivation of virus due to evaporation of droplets, suggested that powder formulations (without inactivation due to evaporation) might allow a significant reduction of vaccine dose, thereby offering new options for fine aerosol vaccination with low-titre vaccines.
authors.affiliation: Laboratory of Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
authors.forename: E A
authors.initials: EA
authors.lastname: Corbanie
authors.forename: C
authors.initials: C
authors.lastname: Vervaet
authors.forename: J H H
authors.initials: JH
authors.lastname: van Eck
authors.forename: J P
authors.initials: JP
authors.lastname: Remon
authors.forename: W J M
authors.initials: WJ
authors.lastname: Landman
mesh_terms: D000280:Administration, Inhalation; D000336:Aerosols / Q000008:administration & dosage; D000391:Air Microbiology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002645:Chickens; D006385:Hemagglutination Inhibition Tests; D006813:Humidity; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control*; D009522:Newcastle disease virus / Q000276:immunology* / Q000302:isolation & purification; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D011208:Powders / Q000008:administration & dosage; D014611:Vaccination / Q000379:methods*; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000336:Aerosols; D000914:Antibodies, Viral; D011208:Powders; D014765:Viral Vaccines