Glycosylation of daidzein by the Eucalyptus cell cultures.
The sequential glycosylation of a soybean isoflavone, daidzein, with cultured suspension cells of Eucalyptus perriniana and cyclodextrin glucanotransferase was studied. Daidzein was converted into two glycosylation products, daidzein 7-O-beta-D-glucopyranoside (39%) and daidzein 7-O-[6-O-(beta-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-gentiobioside, 6%), by cultured E. perriniana cells. Further glycosylation of daidzein 7-O-beta-glucoside with cyclodextrin glucanotransferase gave daidzein 7-O-[4-O-(alpha-D-glucopyranosyl)]-beta-D-glucopyranoside (beta-maltoside, 26%), daidzein 7-O-beta-maltotrioside (15%), and daidzein 7-O-beta-maltotetraoside (7%).
Shimoda
Kei
K
Department of Pharmacology and Therapeutics, Faculty of Medicine, Oita University, 1-1 Hasama-machi, Oita 879-5593, Japan.
Sato
Noriaki
N
Kobayashi
Tatsunari
T
Hamada
Hatsuyuki
H
Hamada
Hiroki
H
D002478:Cells, Cultured / Q000378:metabolism; D003505:Cyclodextrins / Q000378:metabolism*; D005052:Eucalyptus / Q000166:cytology / Q000378:metabolism*; D005960:Glucosides / Q000378:metabolism; D005964:Glucosyltransferases / Q000378:metabolism; D006031:Glycosylation; D007529:Isoflavones / Q000378:metabolism*; D007700:Kinetics; D010636:Phenols / Q000378:metabolism
D003505:Cyclodextrins; D005960:Glucosides; D007529:Isoflavones; D010636:Phenols; C004742:daidzein; D005964:Glucosyltransferases; C019963:cyclomaltodextrin glucanotransferase