title: A single step method for purification of sulfated oligosaccharides.
abstract: Purifying and analysing sulfated oligosaccharides by mass spectrometry often constitutes a challenge. We present here a single step method to isolate sulfated compounds from a complex mixture of neutral and acidic oligosaccharide-alditols. The strategy relies on the exclusion of sulfated molecules from strong cation exchange resin. By testing a wide range of reduced mucin type O-glycans isolated from different biological sources, we demonstrate that this method permits, without prior chemical modification, the specific purification of sulfate-containing oligosaccharides present in any quantity from very complex mixtures of molecules.
authors.affiliation: Unité de Glycobiologie Structurale et Fonctionnelle, CNRS UMR 8576, USTL, 59655, Villeneuve d'Ascq, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Garenaux
authors.forename: Shin-Yi
authors.initials: SY
authors.lastname: Yu
authors.forename: Doina
authors.initials: D
authors.lastname: Florea
authors.forename: Gérard
authors.initials: G
authors.lastname: Strecker
authors.forename: Kay-Hooi
authors.initials: KH
authors.lastname: Khoo
authors.forename: Yann
authors.initials: Y
authors.lastname: Guérardel
mesh_terms: D000663:Amphibians; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D001980:Bronchi; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange; D006801:Humans; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013402:Sugar Alcohols / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry / Q000302:isolation & purification*; D014132:Trachea
chemical_list: D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols; D013431:Sulfates