Identification of the TFII-I family target genes in the vertebrate genome.
GTF2I and GTF2IRD1 encode members of the TFII-I transcription factor family and are prime candidates in the Williams syndrome, a complex neurodevelopmental disorder. Our previous expression microarray studies implicated TFII-I proteins in the regulation of a number of genes critical in various aspects of cell physiology. Here, we combined bioinformatics and microarray results to identify TFII-I downstream targets in the vertebrate genome. These results were validated by chromatin immunoprecipitation and siRNA analysis. The collected evidence revealed the complexity of TFII-I-mediated processes that involve distinct regulatory networks. Altogether, these results lead to a better understanding of specific molecular events, some of which may be responsible for the Williams syndrome phenotype.
Chimge
Nyam-Osor
NO
Department of Molecular, Cellular, and Craniofacial Biology, Birth Defects Center, University of Louisville, 501 South Preston Street, Louisville, KY 40292, USA.
Makeyev
Aleksandr V
AV
Ruddle
Frank H
FH
Bayarsaihan
Dashzeveg
D
D000818:Animals; D001483:Base Sequence; D019295:Computational Biology; D016384:Consensus Sequence; D005347:Fibroblasts / Q000378:metabolism; D005786:Gene Expression Regulation; D016678:Genome / Q000235:genetics*; D006801:Humans; D051379:Mice; D008969:Molecular Sequence Data; D009124:Muscle Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000378:metabolism; D015203:Reproducibility of Results; D015534:Trans-Activators / Q000378:metabolism; D019473:Transcription Factors, TFII / Q000235:genetics*; D014714:Vertebrates / Q000235:genetics*
C104259:GTF2I protein, human; C486330:Gtf2i protein, mouse; C486558:Gtf2ird1 protein, mouse; D009124:Muscle Proteins; D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D015534:Trans-Activators; D019473:Transcription Factors, TFII