[Development of full-quantified fingerprint for quality control of traditional Chinese medicines].
Full-quantified fingerprint technique combines the fingerprint technique and multiple-target determination technique. The development of full-quantified fingerprint technique includes the preparation of full-quantified fraction, the quality control in preparation process with fingerprint technique and determination of products. In this paper, the full-quantified fractions of Salvia miltiorrhiza Bge. were prepared by water extraction, deposition with the ethanol, membrane filter, separation on macroporous resin and separation with preparative high performance liquid chromatography (HPLC). Fingerprint technique was used to test the reproducibility of preparation. Three ingredients, protocatechualdehyde, rosmarinic acid, salvianolic acid B were determined and the sum of their contents was more than 50%. The pharmaceutical active compounds were taken as the preparation target. The impurities were removed effectively by multiple preparations, and the specification of full-quantified fraction was improved greatly.
Feng
Jiatao
J
Dalian Institute of Chemical Physics, the Chinese Academy of Sciences, Dalian 116023, China.
Jin
Yu
Y
Wang
Jincheng
J
Xiao
Yuansheng
Y
Liang
Xinmiao
X
D001892:Camphanes; D002845:Chromatography / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000032:analysis* / Q000737:chemistry* / Q000592:standards; D057230:Limit of Detection; D016014:Linear Models; D008516:Medicine, Chinese Traditional / Q000592:standards*; D053723:Panax notoginseng; D011786:Quality Control; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza
D001892:Camphanes; D004365:Drugs, Chinese Herbal; C000604051:danshen dripping pill