Antigenic studies on mouse lymphomas bearing the T190 translocation chromosome.
Heterozygous mice of the T190/tf translocation stock, having one superlong and one short marker chromosome, involving the H-2-bearing ninth linkage group, were typed by membrane fluorescence and cytotoxicity tests against a panel of H-2 antisera. Marker-positive segregants differed serologically from their marker-negative siblings. Lymphomas were induced by the Moloney virus in (A.CA x T190/tf)F1 mice. A combined serological and cytogenetic analysis showed that the tumors could be subdivided into two main classes, corresponding to marker-positive and marker-negative types. Each lymphoma was relatively stable with regard to karyologic and correlated antigenic characteristics during serial passage in (A.CA x T190/tf)F1 mice. This system opens new possibilities to study the genetic mechanisms of H-2 antigenic variation, demonstrated previously in heterozygous tumors.
Bjaring
B
B
Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Bregula
U
U
Klein
G
G
Levan
A
A
D000818:Animals; D000916:Antibody Diversity; D000951:Antigens, Neoplasm / Q000276:immunology*; D000956:Antigens, Viral / Q000276:immunology; D033481:Chromosomes, Mammalian / Q000235:genetics*; D003601:Cytotoxicity Tests, Immunologic; D019084:Fluorescent Antibody Technique, Indirect; D006183:H-2 Antigens / Q000276:immunology*; D007106:Immune Sera; D007621:Karyotyping; D008223:Lymphoma / Q000235:genetics* / Q000276:immunology* / Q000821:virology; D051379:Mice; D008805:Mice, Inbred A; D008979:Moloney murine leukemia virus; D014178:Translocation, Genetic
D000951:Antigens, Neoplasm; D000956:Antigens, Viral; D006183:H-2 Antigens; D007106:Immune Sera