Helicase translocation assay method using avidin and biotinylated nucleotides.
Helicase involves many cellular processes that separate double-stranded nucleic acid into single strands. Although it is believed that helicase translocates nucleic acids, it is difficult to show the direct evidence of translocation on nucleic acids. In this study, an avidin-biotinylated nucleotides-based method for helicase translocation assay has been described, and the biochemical assay results have been demonstrated.
Shin
Jae-Ho
JH
School of Applied Biosciences, Kyungpook National University, Daegu, 702-701, South Korea. jhshin@knu.ac.kr
D001360:Avidin / Q000737:chemistry / Q000378:metabolism*; D019921:Biotinylation; D004265:DNA Helicases / Q000737:chemistry / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry / Q000378:metabolism; D004259:DNA-Directed DNA Polymerase / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D009711:Nucleotides / Q000737:chemistry / Q000378:metabolism*
D004277:DNA, Single-Stranded; D009711:Nucleotides; D001360:Avidin; D004259:DNA-Directed DNA Polymerase; D004265:DNA Helicases