A single step method for purification of sulfated oligosaccharides.
Purifying and analysing sulfated oligosaccharides by mass spectrometry often constitutes a challenge. We present here a single step method to isolate sulfated compounds from a complex mixture of neutral and acidic oligosaccharide-alditols. The strategy relies on the exclusion of sulfated molecules from strong cation exchange resin. By testing a wide range of reduced mucin type O-glycans isolated from different biological sources, we demonstrate that this method permits, without prior chemical modification, the specific purification of sulfate-containing oligosaccharides present in any quantity from very complex mixtures of molecules.
Garenaux
Estelle
E
Unité de Glycobiologie Structurale et Fonctionnelle, CNRS UMR 8576, USTL, 59655, Villeneuve d'Ascq, France.
Yu
Shin-Yi
SY
Florea
Doina
D
Strecker
Gérard
G
Khoo
Kay-Hooi
KH
Guérardel
Yann
Y
D000663:Amphibians; D000818:Animals; D001671:Biochemistry / Q000379:methods*; D001980:Bronchi; D002236:Carbohydrate Conformation; D002240:Carbohydrate Sequence; D005591:Chemical Fractionation; D002852:Chromatography, Ion Exchange; D006801:Humans; D008969:Molecular Sequence Data; D009077:Mucins / Q000737:chemistry; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013402:Sugar Alcohols / Q000737:chemistry; D013431:Sulfates / Q000737:chemistry / Q000302:isolation & purification*; D014132:Trachea
D009077:Mucins; D009844:Oligosaccharides; D013402:Sugar Alcohols; D013431:Sulfates