Optimized substrate concentrations for production of long-chain isomaltooligosaccharides using dextransucrase of Leuconostoc mesenteroides B-512F.
Isomaltooligosaccharide (IMO) is a promising dietary component with prebiotic effect, and the long-chain IMOs are preferred to short chain ones owing to the longer persistence in the colon. To establish the optimal process for synthesis of long-chain IMOs, we systematically examined the reaction condition of dextransucrase of Leuconostoc mesenteroides B-512F by changing the ratio of sucrose to maltose (varying as 1:4, 1:2, 1:1, and 2:1) and amount of each sugar (from 2% to 20%). As a result, a ratio of 2:1 (sucrose to maltose, 10:5% or 20:10%, w/v) was determined as an optimal condition for long-chain IMO synthesis (DP3-DP9) with relatively higher yields (70-90%, respectively).
Lee
Min Sung
MS
Department of Food Science and Technology, Research Center for Bioresource and Health, Chungbuk National University, Cheongju, Korea.
Cho
Seung Kee
SK
Eom
Hyun-Ju
HJ
Kim
So-Young
SY
Kim
Tae-Jip
TJ
Han
Nam Soo
NS
D003470:Culture Media; D005285:Fermentation; D005964:Glucosyltransferases / Q000378:metabolism*; D007934:Leuconostoc / Q000201:enzymology*; D008320:Maltose / Q000378:metabolism; D009844:Oligosaccharides / Q000096:biosynthesis*; D013379:Substrate Specificity; D013395:Sucrose / Q000378:metabolism
D003470:Culture Media; D009844:Oligosaccharides; C021705:maltooligosaccharides; D013395:Sucrose; D008320:Maltose; D005964:Glucosyltransferases; C020025:dextransucrase