title: Proteomics as a tool to investigate cell models for dopamine toxicity.
abstract: Dopaminergic neurons are particularly exposed to oxidative stress because dopamine metabolism gives rise to endogenous toxins. Here, two different models for dopamine toxicity have been investigated using proteomics: stable alpha-synuclein-expressing neuroblastoma (SH-SY5Y) cells to understand how alpha-synuclein modulates dopamine toxicity at the central level, and cultured T-cell leukaemia (Jurkat) cells challenged with dopamine or l-dopa to evaluate protein changes on the surface of peripheral cells. alpha-synuclein-expressing cells were more resistant compared with wild-type cells, showing upregulation of antioxidant proteins including the Parkinson's disease-related DJ-1 protein. In addition, treatment of prototypical T-cells with l-dopa induced significant changes in proteins on the cell surface, indicating that peripheral blood lymphocytes may be good indicators of dopamine toxicity.
authors.affiliation: Department of Structural and Functional Biology, University of Insubria, Via Alberto da Giussano 12, Busto Arsizio (VA), Italy. mauro.fasano@uninsubria.it
authors.forename: Mauro
authors.initials: M
authors.lastname: Fasano
authors.forename: Tiziana
authors.initials: T
authors.lastname: Alberio
authors.forename: Monica
authors.initials: M
authors.lastname: Colapinto
authors.forename: Silvia
authors.initials: S
authors.lastname: Mila
authors.forename: Leonardo
authors.initials: L
authors.lastname: Lopiano
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004298:Dopamine / Q000633:toxicity*; D006801:Humans; D009447:Neuroblastoma / Q000473:pathology; D020258:Neurotoxicity Syndromes / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D040901:Proteomics / Q000379:methods*
chemical_list: D004298:Dopamine