Purification, crystallization and preliminary X-ray crystallographic analysis of Rab27a GTPase in complex with exophilin4/Slp2-a effector.
By switching between GTP-active and GDP-inactive conformations, small Ras GTPases partly regulate membrane trafficking, cell growth and cytoskeleton dynamics. Among Rab GTPases, the Rab27 subfamily, which comprises Rab27a and Rab27b, controls the proper targeting of secretory vesicles to the plasma membrane. GppNHp-bound Rab27a in complex with the Rab27-binding domain of exophilin4/Slp2-a effector has been purified and crystallized for structural studies. The crystals belong to space group P2(1)2(1)2(1) and a complete data set was collected to a resolution of 1.8 A. Eventually, the structural characterization of the Rab27a-exophilin4/Slp2-a complex will clarify Rab27 recognition by its effectors prior to vesicle tethering and docking.
Chavas
Leonard M G
LM
Faculty of Life Sciences, The University of Manchester, Michael Smith Building, Oxford Road, Manchester, Lancashire M13 9PT, England. leonard.chavas@manchester.ac.uk
Ihara
Kentaro
K
Kawasaki
Masato
M
Kato
Ryuichi
R
Izumi
Tetsuro
T
Wakatsuki
Soichi
S
D000818:Animals; D003460:Crystallization; D018360:Crystallography, X-Ray; D022162:Cytoplasmic Vesicles / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D051379:Mice; D011485:Protein Binding / Q000235:genetics; D017434:Protein Structure, Tertiary; D020691:rab GTP-Binding Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D000075745:rab27 GTP-Binding Proteins
D008565:Membrane Proteins; C424134:Sytl2 protein, mouse; D000075745:rab27 GTP-Binding Proteins; C512962:Rab27a protein, mouse; D020691:rab GTP-Binding Proteins