Inhibitory effects of salvianolic acid B on the high glucose-induced mesangial proliferation via NF-kappaB-dependent pathway.
Salvianolic acid B (Sal B) is one of the major water-soluble compounds isolated from Radix Salviae Miltiorrhizae (Danshen in Chinese) that has been reported to be beneficial to treatment of diabetic complications. However, the mechanisms involved in these effects are not discussed in relation to mesangial proliferation via modulation of NF-kappaB. To explain this, human mesangial cells were pretreated with or without Sal B (0.1, 1, 10 microM) for 24 h and stimulated with high glucose (30 mM). Then the effects of Sal B on mesangial cells proliferation, extracellular matrix production and the possible mechanisms were evaluated by methylthiazoletetrazolium assay, flow cytometry assay, enzyme-linked immunosorbent assay, gelatin zymography assay and western blot assay. These results indicated that Sal B could inhibit high glucose-induced mesangial cells proliferation and extracellular matrix production in a dose-dependent manner, partially through modulating the cell-cycle progress and MMP-2 and MMP-9 activities via suppressing NF-kappaB activation, suggesting that Sal B may be a promising agent for treating diabetic nephropathy.
Luo
Pei
P
West China School of Pharmacy, Sichuan University, Sichuan, PR China.
Tan
Zhenghuai
Z
Zhang
Zhifeng
Z
Li
Honghao
H
Mo
Zhengji
Z
D000975:Antioxidants / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D015153:Blotting, Western; D002453:Cell Cycle / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005353:Fibronectins / Q000378:metabolism; D005434:Flow Cytometry; D005947:Glucose / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis; D020780:Matrix Metalloproteinase 9 / Q000096:biosynthesis; D050527:Mesangial Cells / Q000187:drug effects*; D016328:NF-kappa B / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects; D013778:Tetrazolium Salts; D013844:Thiazoles; D053630:Depsides
D000975:Antioxidants; D001572:Benzofurans; D005353:Fibronectins; D005947:Glucose; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9; D016328:NF-kappa B; D013778:Tetrazolium Salts; D013844:Thiazoles; C076944:salvianolic acid B; D053630:Depsides; C022616:thiazolyl blue