Antibody suspension bead arrays within serum proteomics.
Antibody microarrays offer a powerful tool to screen for target proteins in complex samples. Here, we describe an approach for systematic analysis of serum, based on antibodies and using color-coded beads for the creation of antibody arrays in suspension. This method, adapted from planar antibody arrays, offers a fast, flexible, and multiplexed procedure to screen larger numbers of serum samples, and no purification steps are required to remove excess labeling substance. The assay system detected proteins down to lower picomolar levels with dynamic ranges over 3 orders of magnitude. The feasibility of this workflow was shown in a study with more than 200 clinical serum samples tested for 20 serum proteins.
Schwenk
Jochen M
JM
Department of Proteomics, School of Biotechnology, KTH Royal Institute of Technology, Albanova University Center, Stockholm, Sweden. schwenk@kth.se
Gry
Marcus
M
Rimini
Rebecca
R
Uhlén
Mathias
M
Nilsson
Peter
P
D000906:Antibodies; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis* / Q000276:immunology; D001798:Blood Proteins / Q000032:analysis* / Q000276:immunology; D005240:Feasibility Studies; D006801:Humans; D040081:Protein Array Analysis; D020543:Proteome / Q000032:analysis* / Q000276:immunology; D012680:Sensitivity and Specificity; D044967:Serum
D000906:Antibodies; D000941:Antigens; D001798:Blood Proteins; D020543:Proteome