title: Validated HPTLC methods for the determination of salicin in Salix sp. and of harpagoside in Harpagophytum [corrected] procumbens.
abstract: Preparations of Harpagophytum procumbens and of Salix species are successfully used for the treatment of degenerative rheumatism and painful arthrosis. For the quality control of both drugs, rapid methods of quantification are desirable. Here we report the development of two HPTLC methods enabling the determination of harpagoside in Harpagophyti radix and of salicin in Salicis cortex. We focused on a standardized methodology and thorough validation including two laboratories. The methods allow the analyses of up to 16 samples in parallel demonstrating the proposed methods as very rapid and cost efficient.
authors.affiliation: Institute of Pharmaceutical Sciences, Department of Pharmaceutical Biology and Biotechnology, Albert-Ludwigs-University Freiburg, 79104 Freiburg, Germany.
authors.forename: Steffen
authors.initials: S
authors.lastname: Wagner
authors.forename: Abraham
authors.initials: A
authors.lastname: Ureña
authors.forename: Eike
authors.initials: E
authors.lastname: Reich
authors.forename: Irmgard
authors.initials: I
authors.lastname: Merfort
mesh_terms: D001592:Benzyl Alcohols / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D005960:Glucosides; D006027:Glycosides / Q000032:analysis*; D031684:Harpagophytum / Q000737:chemistry*; D015394:Molecular Structure; D018517:Plant Roots / Q000737:chemistry; D011714:Pyrans / Q000032:analysis*; D015203:Reproducibility of Results; D032108:Salix / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D001592:Benzyl Alcohols; D005960:Glucosides; D006027:Glycosides; D011714:Pyrans; C005696:salicin; C033249:harpagoside