title: Infections with beta-haemolytic streptococci: detection by a universal PCR for bacterial DNA and DNA sequencing.
abstract: PCR using universal bacterial primers and subsequent DNA sequence analysis of the PCR product was used to detect beta-haemolytic streptococci in clinical samples. Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus dysgalactiae were identified in 19 primary culture-negative cases of infections from 203 patient samples with detectable bacterial DNA.
authors.affiliation: Department of Bacteriology, Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark. ccg@ssi.dk
authors.forename: Cecilie
authors.initials: C
authors.lastname: Grarup
authors.forename: Ann Sofie
authors.initials: AS
authors.lastname: Gleesen
authors.forename: Rimtas
authors.initials: R
authors.lastname: Dargis
authors.forename: Keld
authors.initials: K
authors.lastname: Andresen
authors.forename: Jens Jørgen
authors.initials: JJ
authors.lastname: Christensen
authors.forename: Michael
authors.initials: M
authors.lastname: Kemp
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013291:Streptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial