Preventive effect of Ganoderma amboinense on acetaminophen-induced acute liver injury.
In vivo preventive effects of Ganoderma amboinense against acetaminophen-induced hepatotoxicity in Balb/cA mice were studied. G. amboinense powder at 1% and 2% was mixed with standard diet and supplied to mice for 6 weeks, and followed by acetaminophen (350 mg/kg body weight) intraperitoneal injection. In normal mice (without acetaminophen treatment), the consumption of G. amboinense significantly increased hepatic glutathione (GSH) level. Acetaminophen treatment significantly elevated both alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities; however, the pre-intake of G. amboinense significantly and dose-dependently protected liver against the subsequent acetaminophen-induced elevation of ALT and AST activities. Acetaminophen treatment also caused significant GSH depletion, malondialdehyde (MDA) and reactive oxygen species (ROS) increase, and activity reduction of glutathione peroxidase (GPX) and catalase. However, the pre-intake of G. amboinense significantly diminished the subsequent acetaminophen-induced GSH depletion, MDA and ROS increase, and retarded the loss of catalase and GPX activities, in which the effect of G. amboinense on GPX activity, and formation of MDA and ROS was dose-dependent. These results support that G. amboinense may be considered as a preventive agent for acute liver injury.
Hsu
Cheng-chin
CC
Department of Nutritional Science, Chung Shan Medical University, Taichung City, Taiwan.
Lin
Ko-yen
KY
Wang
Zhi-hong
ZH
Lin
Wea-lung
WL
Yin
Mei-chin
MC
D000082:Acetaminophen / Q000009:adverse effects*; D000410:Alanine Transaminase / Q000378:metabolism; D000818:Animals; D001219:Aspartate Aminotransferases / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D056486:Chemical and Drug Induced Liver Injury / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000032:analysis; D032902:Ganoderma / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D059808:Polyphenols / Q000032:analysis; D020011:Protective Agents / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism
D005419:Flavonoids; D059808:Polyphenols; D020011:Protective Agents; D017382:Reactive Oxygen Species; D000082:Acetaminophen; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione