Microchamber arrays for the identification of individual cells exposed to an X-ray microbeam.
To identify individual cells exposed to a X-ray microbeam in a cell population, we developed a biocompatible microchamber-array chip using UV lithography of photopolymer SU-8. The center-to-center distance between microchambers is 50 mum including a wall of 15 mum height. Using the microchamber-array chip, we performed tracking of individual exposed cells. Sample cells loaded in a microchamber array were selectively irradiated with the X-ray microbeam under microscopic observation. All the irradiated cells were indexed by the array arrangement of the microchambers. For about 24 h of post-irradiation incubation, the irradiated cells were identified successfully by time-lapse observation. In addition, the induction of radiation effects was observed in identified cells using immunofluorescence.
Kuchimaru
Takahiro
T
Division of Electric, Electronic and Information Engineering, Graduate School of Engineering, Osaka University, Suita, Osaka 567-0871, Japan. kuchimaru@nf.eie.eng.osaka-u.ac.jp
Sato
Fuminobu
F
Aoi
Yusuke
Y
Fujita
Tomohisa
T
Ikeda
Toshiji
T
Shimizu
Kikuo
K
Kato
Yushi
Y
Iida
Toshiyuki
T
D000818:Animals; D018929:Cell Culture Techniques / Q000295:instrumentation*; D002469:Cell Separation / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D046228:Microarray Analysis / Q000295:instrumentation*; D009474:Neurons / Q000502:physiology* / Q000528:radiation effects*; D016716:PC12 Cells; D011829:Radiation Dosage; D011874:Radiometry / Q000295:instrumentation*; D051381:Rats; D014965:X-Rays