Interleukin-3 (IL-3)-induced c-fos activation is modulated by Gab2-calcineurin interaction.
Interleukin-3 (IL-3) regulates cell growth by affecting various processes such as cell death, survival, and proliferation. Cues from the external environment are sensed by surface receptors, and complex signaling mechanisms arise within the cells, leading to specific functional outcomes. In this study, we demonstrate that the cytokine IL-3 induces the activation of the Ca(2+)-dependent phosphatase, calcineurin (Cn). Furthermore Cn dephosphorylates Gab2, resulting in c-fos activation and cell proliferation. We also report that there is a direct interaction between Cn and Gab2 upon IL-3 stimulation, and Akt can regulate this interaction.
Pyarajan
Saiju
S
Cancer Institute, NYU School of Medicine, New York University, New York, NY 10016, USA. saiju.pyarajan@mssm.edu
Matejovic
Gabriel
G
Pratt
Joanne C
JC
Baksh
Shairaz
S
Burakoff
Steven J
SJ
D048868:Adaptor Proteins, Signal Transducing; D000818:Animals; D019703:Calcineurin / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D016923:Cell Death / Q000502:physiology; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D004789:Enzyme Activation / Q000502:physiology; D007377:Interleukin-3 / Q000378:metabolism*; D051379:Mice; D010750:Phosphoproteins / Q000378:metabolism*; D010766:Phosphorylation; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology*
D048868:Adaptor Proteins, Signal Transducing; C490894:Gab2 protein, mouse; D007377:Interleukin-3; D010750:Phosphoproteins; D016760:Proto-Oncogene Proteins c-fos; D051057:Proto-Oncogene Proteins c-akt; D019703:Calcineurin; D002118:Calcium