title: Kinetic studies and unstructured models of lymphocyte metabolism in fed-batch culture.
abstract: The growth of two lymphocyte cell lines, a hybridoma cell line and a human cutaneous T cell lymphoma (HuT78), was studied in fed-batch culture, and unstructured models of growth developed. A criteria was established to insure that the growth rate varied by less than a specified tolerance throughout the culture period. Glutamine and serum were growth-limiting nutrients for both cell lines with half-maximal growth rates at 0. 53 mM glutamine and 0. 55%(v/v) serum for the hybridoma cells and 0. 21 mM glutamine and 1. 5% serum for the HuT-78 cells. Over the range of glucose concentrations from 5. 5 mM to 28 mM, the specific growth rate of hybridoma cells was independent of glucose concentration, whereas glucose concentrations above 5. 5 mM inhibited HuT-78 growth. For both cell lines, the growth rate was significantly inhibited by the addition of ammonium, although the hybridoma cell line was more affected by ammonia than was the HuT-78 cell line. Growth of HuT-78 cells increased in the presence of interleukin-2. Unstructured models for the hybridoma cells were similar to other models presented in the literature. Applications of these models to adoptive immunotherapy are discussed.
authors.affiliation: Department of Chemical Engineering and Biotechnology Engineering Center, Tufts University, Medford, Massachusetts 02155.
authors.forename: G A
authors.initials: GA
authors.lastname: Truskey
authors.forename: D P
authors.initials: DP
authors.lastname: Nicolakis
authors.forename: D
authors.initials: D
authors.lastname: DiMasi
authors.forename: A
authors.initials: A
authors.lastname: Haberman
authors.forename: R W
authors.initials: RW
authors.lastname: Swartz