Preliminary phytochemical and biological study of Cirsium ehrenbergii.
Cirsium ehrenbergii ("red thistle") is a folk medicinal plant of the state of Hidalgo, Mexico. Its flowers and roots are used to prepare an infusion for drinking or applying vaginal douches; nevertheless, neither its secondary metabolites nor therapeutic properties have been described or confirmed. Flowers of C. ehrenbergii, were collected, dried and milled. Aqueous, methanol and hexane extracts were carried out by maceration to obtain polar and non-polar secondary metabolites. The presence of alkaloids was determined by Wagner, Mayer and Dragendorff techniques; both polar and non-polar extracts yielded positive results. Toxicity was quantified by the Artemia salina mortality method, the aqueous extract showed moderate toxicity, while methanol and hexane extracts yielded a very similar and high concentration-dependent mortality. Antibacterial activity was evidenced by cellular growth inhibition of six bacterial strains, wherein the aqueous extract was inactive; the methanol extract was almost ineffective, while the hexane extract showed a high concentration-dependent antibacterial activity on all strains. An isolated organ study was performed with rings of uterus from estrogen-treated female Wistar rats to evaluate relaxing effects on uterine smooth muscle. Rings were pre-contracted with KCl (60 mM). The methanol extract inhibited contraction modestly at the highest concentration (300 pg/mL). The hexane extract markedly inhibited contraction in a concentration-dependent manner. The hexane extract was biologically more effective than the methanol extract. The traditional use of C. ehrenbergii as a medicinal plant may be supported by pharmacological actions.
Fernández-Martínez
Eduardo
E
Centro de Investigación en Biología de la Reproducción del Area Académica de Medicina del Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, Pachuca, Hidalgo, México. efernan@uaeh.reduaeh.mx
Díaz-Espinoza
Rosario
R
Villavicencio-Nieto
Miguel A
MA
Pérez-Escandón
Blanca E
BE
Pérez-Hernández
Nury
N
Macías
Arturo
A
Ortíz
Mario I
MI
Ponce-Monter
Héctor A
HA
D000470:Alkaloids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001156:Artemia; D001419:Bacteria / Q000187:drug effects; D031180:Cirsium / Q000737:chemistry*; D005260:Female; D066298:In Vitro Techniques; D007202:Indicators and Reagents; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D014590:Uterine Contraction / Q000187:drug effects; D014599:Uterus / Q000187:drug effects
D000470:Alkaloids; D000900:Anti-Bacterial Agents; D007202:Indicators and Reagents; D010936:Plant Extracts