[Determination of polydextrose in food by high performance anion exchange chromatographic method with pulsed amperometric detector].
OBJECTIVE
The HPAEC-PAD method for polydextrose determination was developed based on AOAC 2000.11.


METHODS
This method included water extraction, centrifugal ultrafiltration, mulienzyme hydrolysis, and anion exchange chromatography detection. The polydextrose was elated by a gradient program of 0.15 mol/L NaOH and 0.5mol/L sodium acetate in 0.15 mol/L NaOH on a CarboPAC TM PA 1 column, then detected by a gold electrode with pulse amperometric detection mold. The inject volume was 20 microl.


RESULTS
The LOD and LOQ of this method were 1.69 microg/g, 5.47 microg/g, respectively. The repeatability and reproducibility were excellent, ranging from 2.10% to 6.62%. The average recovery of polydextrose in various food matrix were 92.4%-104.4%.


CONCLUSIONS
This method could be used for polydextrose determination in foods.
Li
Jianwen
J
Instituted for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wang
Guodong
G
Yang
Yuexin
Y
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004563:Electrochemistry / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D005936:Glucans / Q000032:analysis*
D005936:Glucans; C033375:polydextrose