[Dynamic changes of LDH and HBDH activity in rabbit serum after low voltage electrical injury].
OBJECTIVE
To investigate changes of LDH and HBDH activity in rabbit serum after non-thermal low voltage electrical injury and to provide diagnostic criteria for non-thermal low voltage electrical injury.


METHODS
Forty New Zealand rabbits were randomly distributed into control group and electrical injury group (EI-groups; designated 7 time points: 0 h, 2 h, 4 h, 12 h, 1 d, 2 d, 3 d), 5 rabbits per each group. EI-groups were treated with the method of non-thermal low voltage electrical injury established in our laboratory. Ventricular blood (5 mL) was obtained under anesthesia at designated time points after electrical injury. The activities of LDH and HBDH were measured.


RESULTS
Dynamic changes were observed with certain patterns from target serum enzyme activities after electrical injury. Compared with control group, the activities of LDH increased markedly at 4 h, 12 h, and on days 1, 2, and 3 after injury (4 h, 12 h, and day 1 P<0.01; day 2 and day 3 P<0.05). Activities of HBDH increased markedly at day 2 and day 3 after injury (P<0.05). The ratio of HBDH/LDH decreased markedly at 2 h, 4 h, and 12 h after injury (P<0.01).


CONCLUSIONS
Dynamic changes of LDH and HBDH activities may be useful in diagnosis of non-thermal low voltage electrical injury and in estimation of post injury intervals.
Xu
Yun-Qiang
YQ
Second Hospital of Shijiazhuang, Shijiazhuang 050051, China. xuyunqiang9@sina.com
Jin
Yan-Kui
YK
Ren
Jun-Qing
JQ
Zuo
Min
M
Wang
Song-Jun
SJ
D000818:Animals; D004556:Electric Injuries / Q000201:enzymology*; D005260:Female; D049429:Forensic Pathology; D006884:Hydroxybutyrate Dehydrogenase / Q000097:blood*; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008297:Male; D011817:Rabbits; D011897:Random Allocation
D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase