title: Transfusion of IgG-opsonized foreign red blood cells mediates reduction of antigen-specific B cell priming in a murine model.
abstract: Hemolytic disease of the fetus and newborn can be effectively prevented by administration of anti-D to the mother. The administered IgG results in the attenuation of RBC-specific Ab production, a process termed Ab-mediated immune suppression (AMIS). Because in animal models of AMIS no major effect on T cell priming occurs, we hypothesized that the effect of the IgG on the immune system under AMIS conditions may involve a deficiency in B cell priming. We therefore challenged mice with either untreated RBCs or IgG-opsonized RBCs (AMIS) and assessed B cell priming. B cells from mice transfused with untreated RBCs, but not from mice treated under AMIS conditions, were primed as assessed by their ability to function as Ag-specific APCs to appropriate T cells. To our knowledge, this is the first report demonstrating that AMIS inhibits the appearance of Ag-primed RBC-specific B cells.
authors.affiliation: Department of Laboratory Medicine of St Michael's Hospital, Canadian Blood Services, Keenan Research Centre, Li Ka Shing Knowledge Institute, Toronto, Ontario, Canada.
authors.forename: Davor
authors.initials: D
authors.lastname: Brinc
authors.forename: Hoang
authors.initials: H
authors.lastname: Le-Tien
authors.forename: Andrew R
authors.initials: AR
authors.lastname: Crow
authors.forename: Vinayakumar
authors.initials: V
authors.lastname: Siragam
authors.forename: John
authors.initials: J
authors.lastname: Freedman
authors.forename: Alan H
authors.initials: AH
authors.lastname: Lazarus
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D049109:Cell Proliferation; D002478:Cells, Cultured; D015536:Down-Regulation; D017707:Erythrocyte Transfusion; D004912:Erythrocytes / Q000276:immunology* / Q000378:metabolism; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology*; D007165:Immunosuppression Therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D023421:Models, Animal; D009895:Opsonin Proteins / Q000276:immunology; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D007074:Immunoglobulin G; D009895:Opsonin Proteins