Hematopoietic differentiation of embryonic stem cells.
This review is focused on methods that are used to derive hematopoietic cells from embryonic stem cells (ESCs). One of the strategies that have been recently used to achieve this goal is an approach of mimicking the hematopoietic niche in vitro by using hematopoiesis-supportive feeder cells, cocktails of soluble hematopoietic growth factors and a variety of matrices. While there is clear evidence that it is possible to derive hematopoietic stem cells (HSCs) and subsequently committed hematopoietic progenitors and mature cells from ESCs, there remains the need to address multiple issues including the efficiency of HSCs derivation in vitro and their proper functionality.
Orlovskaya
Irina
I
Institute for Clinical Immunology, Yadrinzovskaya 14, Novosibirsk 630090, Russian Federation.
Schraufstatter
Ingrid
I
Loring
Jeanne
J
Khaldoyanidi
Sophia
S
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology*; D002460:Cell Line / Q000378:metabolism; D019070:Cell Lineage / Q000235:genetics; D002469:Cell Separation / Q000379:methods; D003470:Culture Media / Q000494:pharmacology / Q000592:standards; D015194:Diffusion Chambers, Culture; D053595:Embryonic Stem Cells / Q000187:drug effects / Q000502:physiology; D006410:Hematopoiesis; D006412:Hematopoietic Stem Cells / Q000166:cytology* / Q000502:physiology*; D006413:Hematopoietic System / Q000502:physiology; D006801:Humans; D051379:Mice; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects
D003470:Culture Media