title: PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells.
abstract: Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including alpha-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.
authors.affiliation: Department of Animal Science and Biotechnology, Kyungpook National University, Daegu, South Korea.
authors.forename: Chan Ho
authors.initials: CH
authors.lastname: Jang
authors.forename: In Ae
authors.initials: IA
authors.lastname: Lee
authors.forename: Young Ran
authors.initials: YR
authors.lastname: Ha
authors.forename: Jinkyu
authors.initials: J
authors.lastname: Lim
authors.forename: Mi-Kyung
authors.initials: MK
authors.lastname: Sung
authors.forename: Sung-Joon
authors.initials: SJ
authors.lastname: Lee
authors.forename: Jong-Sang
authors.initials: JS
authors.lastname: Kim
mesh_terms: D000872:Anthocyanins / Q000494:pharmacology; D000975:Antioxidants / Q000494:pharmacology*; D015415:Biomarkers / Q000032:analysis; D045744:Cell Line, Tumor; D003110:Colonic Neoplasms / Q000378:metabolism* / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D018384:Oxidative Stress; D010735:Phosphoglycerate Kinase / Q000235:genetics*; D040901:Proteomics
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D015415:Biomarkers; D006861:Hydrogen Peroxide; D010735:Phosphoglycerate Kinase; C017185:delphinidin