Improved degradation of organophosphate dichlorvos by Trichoderma atroviride transformants generated by restriction enzyme-mediated integration (REMI).
A simple technique, REMI (restriction enzyme-mediated integration), was used to construct transformants of Trichoderma atroviride with improved capability of degrading organophosphate pesticide dichlorvos. Linearized DNA of plasmid pV2 bearing the hygromycin B phosphotransferase (hph) gene was inserted into chromosomes of wild strain T23 and transformation was confirmed by PCR and Southern blot analysis, respectively. Of 247 transformants, 76% showed improved dichlorvos degradation ability as compared to the parent strain T23 based on the least significant difference (LSD) test at p=0.01. Among them, 8 transformants exhibited 30% higher in degradation rate than the parent isolate. The highest dichlorvos degradation rate of the transformants was up to 96%. This study provided an effective approach for improving organophosphate pesticide-degrading capability of T. atroviride.
Tang
Jun
J
Department of Plant Science, School of Agriculture and Biology, Key laboratory of Microorganism Metabolism, Ministry of Education, Shanghai Jiaotong University, Shanghai, China.
Liu
Lixing
L
Hu
Shifeng
S
Chen
Yunpeng
Y
Chen
Jie
J
D001673:Biodegradation, Environmental; D004006:Dichlorvos / Q000378:metabolism*; D024861:Genetic Enhancement / Q000379:methods*; D009154:Mutation; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism*; D015183:Restriction Mapping; D014242:Trichoderma / Q000235:genetics* / Q000378:metabolism*
D004006:Dichlorvos; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase