title: Identification of proteomic biomarkers of preeclampsia in amniotic fluid using SELDI-TOF mass spectrometry.
abstract: OBJECTIVE
  To identify proteomic biomarkers in amniotic fluid (AF) that can distinguish preeclampsia (PE) from chronic hypertension (CHTN) and normotensive controls (CTR).
  
  
  METHODS
  AF from women with PE, CHTN, and CTR were subjected to proteomic analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.
  
  
  RESULTS
  Proteomic profiling of AF identified 2 biomarkers: peak X (17399.11 Da), which distinguished PE from CTR, and peak Y (28023.34 Da), which distinguished PE and CHTN from CTR. High performance liquid chromatography fractions containing the biomarkers were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in-gel tryptic digestion. The biomarkers were matched to proapolipoprotein A-I (peak Y) and a functionally obscure peptide, SBBI42 (peak X). Western blot analysis confirmed that AF from PE and CHTN had higher proapolipoprotein A-I levels than CTR.
  
  
  CONCLUSIONS
  Proteomic analysis of AF can distinguish PE from CHTN and CTR. The discriminatory proteins were identified as proapolipoprotein A-I and SBBI42.
authors.affiliation: Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. jsparkmd@snu.ac.kr
authors.forename: Joong Shin
authors.initials: JS
authors.lastname: Park
authors.forename: Kyoung-Jin
authors.initials: KJ
authors.lastname: Oh
authors.forename: Errol R
authors.initials: ER
authors.lastname: Norwitz
authors.forename: Joong-Soo
authors.initials: JS
authors.lastname: Han
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Choi
authors.forename: Hyo Suk
authors.initials: HS
authors.lastname: Seong
authors.forename: Yoon Dan
authors.initials: YD
authors.lastname: Kang
authors.forename: Chan-Wook
authors.initials: CW
authors.lastname: Park
authors.forename: Byoung Jae
authors.initials: BJ
authors.lastname: Kim
authors.forename: Jong Kwan
authors.initials: JK
authors.lastname: Jun
authors.forename: Hee Chul
authors.initials: HC
authors.lastname: Syn
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry* / Q000378:metabolism; D016632:Apolipoprotein A-I / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D006973:Hypertension / Q000175:diagnosis / Q000378:metabolism; D007231:Infant, Newborn; D010865:Pilot Projects; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000378:metabolism*; D011247:Pregnancy; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D016632:Apolipoprotein A-I; D015415:Biomarkers