Selection of recombinant antibodies by eukaryotic ribosome display.
Ribosome display is a powerful method for selection of single-chain antibodies in vitro. It operates through the formation of libraries of antibody-ribosome-mRNA complexes that are selected on immobilized antigen, followed by recovery of the genetic information from the mRNA by RT-PCR. Both prokaryotic and eukaryotic versions are used. We describe our eukaryotic system, in which rabbit reticulocyte extracts are used for cell free transcription/translation and cDNA is recovered by in situ RT-PCR performed on the selected complexes.
He
Mingyue
M
Technology Research Group, The Babraham Institute, Cambridge, UK.
Taussig
Michael J
MJ
D000818:Animals; D000906:Antibodies / Q000737:chemistry*; D005057:Eukaryotic Cells / Q000737:chemistry*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods; D011817:Rabbits; D011994:Recombinant Proteins / Q000737:chemistry* / Q000235:genetics; D012270:Ribosomes / Q000737:chemistry*
D000906:Antibodies; D011994:Recombinant Proteins