title: Heterogeneity and clinical significance of ETV1 translocations in human prostate cancer.
abstract: A fluorescence in situ hybridisation (FISH) assay has been used to screen for ETV1 gene rearrangements in a cohort of 429 prostate cancers from patients who had been diagnosed by trans-urethral resection of the prostate. The presence of ETV1 gene alterations (found in 23 cases, 5.4%) was correlated with higher Gleason Score (P=0.001), PSA level at diagnosis (P=<0.0001) and clinical stage (P=0.017) but was not linked to poorer survival. We found that the six previously characterised translocation partners of ETV1 only accounted for 34% of ETV1 re-arrangements (eight out of 23) in this series, with fusion to the androgen-repressed gene C15orf21 representing the commonest event (four out of 23). In 5'-RACE experiments on RNA extracted from formalin-fixed tissue we identified the androgen-upregulated gene ACSL3 as a new 5'-translocation partner of ETV1. These studies report a novel fusion partner for ETV1 and highlight the considerable heterogeneity of ETV1 gene rearrangements in human prostate cancer.
authors.affiliation: Institute of Cancer Research, Male Urological Cancer Research Centre, 15 Cotswold Road, Sutton, Surrey SM2 5NG, UK.
authors.forename: G
authors.initials: G
authors.lastname: Attard
authors.forename: J
authors.initials: J
authors.lastname: Clark
authors.forename: L
authors.initials: L
authors.lastname: Ambroisine
authors.forename: I G
authors.initials: IG
authors.lastname: Mills
authors.forename: G
authors.initials: G
authors.lastname: Fisher
authors.forename: P
authors.initials: P
authors.lastname: Flohr
authors.forename: A
authors.initials: A
authors.lastname: Reid
authors.forename: S
authors.initials: S
authors.lastname: Edwards
authors.forename: G
authors.initials: G
authors.lastname: Kovacs
authors.forename: D
authors.initials: D
authors.lastname: Berney
authors.forename: C
authors.initials: C
authors.lastname: Foster
authors.forename: C E
authors.initials: CE
authors.lastname: Massie
authors.forename: A
authors.initials: A
authors.lastname: Fletcher
authors.forename: J S
authors.initials: JS
authors.lastname: De Bono
authors.forename: P
authors.initials: P
authors.lastname: Scardino
authors.forename: J
authors.initials: J
authors.lastname: Cuzick
authors.forename: C S
authors.initials: CS
authors.lastname: Cooper
mesh_terms: D003066:Coenzyme A Ligases / Q000235:genetics; D015331:Cohort Studies; D004268:DNA-Binding Proteins / Q000235:genetics*; D050939:Gene Fusion; D015321:Gene Rearrangement; D018740:Genetic Heterogeneity; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D016612:Paraffin Embedding; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000473:pathology; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014157:Transcription Factors / Q000235:genetics*; D014178:Translocation, Genetic; D000098859:Long-Chain-Fatty-Acid-CoA Ligase
chemical_list: D004268:DNA-Binding Proteins; C095239:ETV1 protein, human; D014157:Transcription Factors; D003066:Coenzyme A Ligases; D000098859:Long-Chain-Fatty-Acid-CoA Ligase