title: Effects of different Helicobacter pylori culture filtrates on growth of gastric epithelial cells.
abstract: OBJECTIVE
  To study the effects of different Helicobacter pylori (H pylori) culture filtrates on growth of gastric epithelial cells.
  
  
  METHODS
  Broth culture filtrates of H pylori were prepared. Gastric epithelial cells were treated with the filtrates, and cell growth was determined by growth curve and flow cytometry. DNA damage of gastric epithelial cells was measured by single-cell microgel electrophoresis.
  
  
  RESULTS
  Gastric epithelial cells proliferated actively when treated by CagA-gene-positive broth culture filtrates, and colony formation reached 40%. The number of cells in S phase increased compared to controls. Comet assay showed 41.2% comet cells in GES-1 cells treated with CagA-positive filtrates (P < 0.05).
  
  
  CONCLUSIONS
  CagA-positive filtrates enhance the changes in morphology and growth characteristics of human gastric epithelial tumor cells. DNA damage maybe one of the mechanisms involved in the growth changes.
authors.affiliation: Department of General Surgery, The Affiliated Hospital of Wuhan University of Science & Technology, Wuhan 430064, Hubei Province, China. yan969400@yahoo.com.cn
authors.forename: Yan-Guo
authors.initials: YG
authors.lastname: Yan
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.forename: Jin-Ping
authors.initials: JP
authors.lastname: Ma
authors.forename: Shi-Rong
authors.initials: SR
authors.lastname: Cai
authors.forename: Wen-Hua
authors.initials: WH
authors.lastname: Zhan
mesh_terms: D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D020552:Comet Assay; D017077:Culture Media, Conditioned / Q000378:metabolism; D004249:DNA Damage; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000378:metabolism*; D016480:Helicobacter pylori / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019899:Paracrine Communication; D013997:Time Factors
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; C080866:cagA protein, Helicobacter pylori