title: MR tracking of transplanted cells with "positive contrast" using manganese oxide nanoparticles.
abstract: Rat glioma cells were labeled using electroporation with either manganese oxide (MnO) or superparamagnetic iron oxide (SPIO) nanoparticles. The viability and proliferation of SPIO-labeled cells (1.9 mg Fe/ml) or cells electroporated with a low dose of MnO (100 microg Mn/ml) was not significantly different from unlabeled cells; a higher MnO dose (785 microg Mn/ml) was found to be toxic. The cellular ion content was 0.1-0.3 pg Mn/cell and 4.4 pg Fe/cell, respectively, with cellular relaxivities of 2.5-4.8 s(-1) (R(1)) and 45-84 s(-1) (R(2)) for MnO-labeled cells. Labeled cells (SPIO and low-dose MnO) were each transplanted in contralateral brain hemispheres of rats and imaged in vivo at 9.4T. While SPIO-labeled cells produced a strong "negative contrast" due to the increase in R(2), MnO-labeled cells produced "positive contrast" with an increased R(1). Simultaneous imaging of both transplants with opposite contrast offers a method for MR "double labeling" of different cell populations.
authors.affiliation: Russell H. Morgan Department of Radiology, Division of MR Research, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA.
authors.forename: Assaf A
authors.initials: AA
authors.lastname: Gilad
authors.forename: Piotr
authors.initials: P
authors.lastname: Walczak
authors.forename: Michael T
authors.initials: MT
authors.lastname: McMahon
authors.forename: Hyon Bin
authors.initials: HB
authors.lastname: Na
authors.forename: Jung Hee
authors.initials: JH
authors.lastname: Lee
authors.forename: Kwangjin
authors.initials: K
authors.lastname: An
authors.forename: Taegwhan
authors.initials: T
authors.lastname: Hyeon
authors.forename: Peter C M
authors.initials: PC
authors.lastname: van Zijl
authors.forename: Jeff W M
authors.initials: JW
authors.lastname: Bulte
mesh_terms: D000818:Animals; D017690:Cell Transplantation; D003287:Contrast Media; D018274:Electroporation; D005290:Ferric Compounds; D005910:Glioma; D008279:Magnetic Resonance Imaging / Q000379:methods*; D017895:Manganese Compounds; D053768:Metal Nanoparticles; D010087:Oxides; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured
chemical_list: D003287:Contrast Media; D005290:Ferric Compounds; D017895:Manganese Compounds; D010087:Oxides; C000499:ferric oxide; C027424:manganese oxide