Naturally acquired IgM antibody response to the C-terminal region of the merozoite surface protein 1 of Plasmodium vivax in Korea: use for serodiagnosis of vivax malaria.
The antibody levels against the C-terminal region of the merozoite surface protein 1 of Plasmodium vivax (PvMSP1c) were measured in 276 patients with P. vivax malaria (patient group), 320 malaria-naïve healthy individuals (control group 1), and 70 malaria-naïve individuals with various disorders (control group 2) using the immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA) and the direct sandwich ELISA. To evaluate the antibody response during relapse, 5 relapsed patients were tested using the IgM capture ELISA. The IgM antibodies were negative in 99.7% of control group 1 and in 100% of control group 2; they were positive in 90.6% of the patient group. The total antibody levels were positive in 88.4% of the patient group with the direct sandwich ELISA. The sera from the second malaria episode, i.e., relapsed patients, were 100% positive on the IgM capture ELISA. The results of this study suggest that the IgM capture ELISA may be a useful diagnostic method for P. vivax malaria for both primary infection and relapse.
Yeom
Joon-Sup
JS
Department of Internal Medicine, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Chongno-gu, Seoul 110-746, Republic of Korea. seorak@dreamwiz.com
Kim
Eu-Suk
ES
Lim
Kook-Jin
KJ
Oh
Jae-Hoon
JH
Sohn
Mi-Jin
MJ
Yoo
Seung-Bum
SB
Kim
Eunkyung
E
Bae
Ineui
I
Jung
Yun-Jae
YJ
Park
Jae-Won
JW
D000818:Animals; D000913:Antibodies, Protozoan / Q000096:biosynthesis* / Q000097:blood; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards; D005500:Follow-Up Studies; D006801:Humans; D007075:Immunoglobulin M / Q000096:biosynthesis* / Q000097:blood; D007723:Korea; D016780:Malaria, Vivax / Q000175:diagnosis*; D008403:Mass Screening / Q000379:methods; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D010966:Plasmodium vivax / Q000276:immunology*; D012008:Recurrence; D012680:Sensitivity and Specificity
D000913:Antibodies, Protozoan; D007075:Immunoglobulin M; D020066:Merozoite Surface Protein 1