Molecular characterization and SNP development for the porcine IL6 and IL10 genes.
Different cytokines are secreted in response to specific microbial molecules referred to as pathogen associated molecular patterns (PAMPs). Interleukin 6 (IL6) and interleukin 10 (IL10), both secreted by macrophages and lymphocytes, play a central role in the immunological response. In this work we obtained the genomic structure and complete DNA sequence of the porcine IL6 and IL10 genes and identified polymorphisms in the genomic sequences of these genes on a panel of ten different pig breeds. Comparative intra- and interbreed sequence analysis revealed a total of eight polymorphisms in the porcine IL6 gene and 21 in the porcine IL10 gene, which include single nucleotide polymorphisms (SNPs) and insertion deletion polymorphisms (indels). Additionally, the chromosomal localization of the IL10 gene was determined by FISH and RH mapping.
Daniłowicz
Emilia
E
Institute of Genetics, Vetsuisse Faculty, University of Berne, Berne, Switzerland.
Akouchekian
Mansoureh
M
Drogemuller
Cord
C
Haase
Bianca
B
Leeb
Tosso
T
Kuiper
Heidi
H
Distl
Ottmar
O
Iras
Fugato-Consortium
FC
D000818:Animals; D001483:Base Sequence; D022202:Chromosomes, Artificial, Bacterial; D033481:Chromosomes, Mammalian / Q000235:genetics; D004247:DNA / Q000235:genetics; D017931:DNA Primers; D054643:INDEL Mutation; D017404:In Situ Hybridization, Fluorescence; D016753:Interleukin-10 / Q000235:genetics*; D015850:Interleukin-6 / Q000235:genetics*; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D021101:Radiation Hybrid Mapping; D017422:Sequence Analysis, DNA; D034421:Sus scrofa / Q000235:genetics*
D017931:DNA Primers; D015850:Interleukin-6; D016753:Interleukin-10; D004247:DNA