Characterization of a chromosomal nickel resistance determinant from Klebsiella oxytoca CCUG 15788.
Klebsiella oxytoca CCUG 15788 is resistant to Ni2+ at a concentration of 10 mM and grows in an inducible manner when exposed to lower concentrations of Ni2+. The complete genomic sequence of a 4.2-kb HindIII-digested fragment of this strain was determined from genomic DNA. It was shown to contain four nickel resistance genes (nirA, nirB, nirC, and nirD) encoding transporter and transmembrane proteins for nickel resistance. When the plasmid pKOHI4, encoding nirABCD, was transformed into Escherichia coli JM109, the cells were able to grow in Tris-buffered mineral medium containing 3 mM nickel. TnphoA'-1 insertion mutants in the four nickel genes nirA, nirB, nirC, and nirD showed nickel sensitivity. The nir genes were heterogeneously expressed in E. coli, suggesting functional roles of these genes in nickel resistance.
Park
Jae-Sun
JS
Institute of Global Environment and Department of Biology, Kyung Hee University, Seoul, Korea.
Lee
Sung-Jae
SJ
Rhie
Ho-Gun
HG
Lee
Ho-Sa
HS
D001426:Bacterial Proteins / Q000235:genetics; D002876:Chromosomes, Bacterial; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D041121:Klebsiella oxytoca / Q000187:drug effects* / Q000235:genetics*; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D005810:Multigene Family; D016254:Mutagenesis, Insertional; D009532:Nickel / Q000494:pharmacology*; D016366:Open Reading Frames; D010957:Plasmids
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D009532:Nickel