title: Endothelial adherens junctions control tight junctions by VE-cadherin-mediated upregulation of claudin-5.
abstract: Intercellular junctions mediate adhesion and communication between adjoining cells. Although formed by different molecules, tight junctions (TJs) and adherens junctions (AJs) are functionally and structurally linked, but the signalling pathways behind this interaction are unknown. Here we describe a cell-specific mechanism of crosstalk between these two types of structure. We show that endothelial VE-cadherin at AJs upregulates the gene encoding the TJ adhesive protein claudin-5. This effect requires the release of the inhibitory activity of forkhead box factor FoxO1 and the Tcf-4-beta-catenin transcriptional repressor complex. Vascular endothelial (VE)-cadherin acts by inducing the phosphorylation of FoxO1 through Akt activation and by limiting the translocation of beta-catenin to the nucleus. These results offer a molecular basis for the link between AJs and TJs and explain why VE-cadherin inhibition may cause a marked increase in permeability.
authors.affiliation: FIRC Institute of Molecular Oncology, 20139 Milan, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Taddei
authors.forename: Costanza
authors.initials: C
authors.lastname: Giampietro
authors.forename: Annarita
authors.initials: A
authors.lastname: Conti
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Orsenigo
authors.forename: Ferruccio
authors.initials: F
authors.lastname: Breviario
authors.forename: Valentina
authors.initials: V
authors.lastname: Pirazzoli
authors.forename: Michael
authors.initials: M
authors.lastname: Potente
authors.forename: Christopher
authors.initials: C
authors.lastname: Daly
authors.forename: Stefanie
authors.initials: S
authors.lastname: Dimmeler
authors.forename: Elisabetta
authors.initials: E
authors.lastname: Dejana
mesh_terms: D022005:Adherens Junctions / Q000502:physiology*; D000818:Animals; D015703:Antigens, CD / Q000502:physiology*; D015820:Cadherins / Q000502:physiology*; D002460:Cell Line; D062507:Claudin-5; D042783:Endothelial Cells; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics*; D015398:Signal Transduction; D051820:TCF Transcription Factors / Q000378:metabolism; D019108:Tight Junctions / Q000235:genetics*; D015854:Up-Regulation / Q000235:genetics*; D000099152:Cadherin 5
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D062507:Claudin-5; D000071161:Forkhead Box Protein O1; D051858:Forkhead Transcription Factors; D008565:Membrane Proteins; D051820:TCF Transcription Factors; D000099152:Cadherin 5; C107466:CLDN5 protein, human; C085126:FOXO1 protein, human