title: Drill-assisted genomic DNA extraction from Botrytis cinerea.
abstract: Current DNA extraction protocols for genomic DNA from Botrytis cinerea almost always start with mycelium that has been reduced to powder with liquid N(2) in a mortar, and this makes their application to a large number of samples slow and cumbersome. Here we present an adaptation of an existing method [Möller et al. (1992) Nucleic Acids Res 20: 6115-6116] for which the initial steps have been modified, including the homogenization of the fungus with sand and the aid of a common household drill. This method allows the processing of large number of samples in much shorter times and generates an average of 4 mug DNA per sample, of sufficient quality for use in PCR and Southern blotting.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Universidad de La Laguna, 38206, La Laguna, Tenerife, Spain. cglez@ull.es
authors.forename: Celedonio
authors.initials: C
authors.lastname: González
authors.forename: Judith
authors.initials: J
authors.lastname: Noda
authors.forename: José J
authors.initials: JJ
authors.lastname: Espino
authors.forename: Nélida
authors.initials: N
authors.lastname: Brito
mesh_terms: D020171:Botrytis / Q000235:genetics*; D004271:DNA, Fungal / Q000235:genetics / Q000302:isolation & purification*; D008967:Molecular Biology / Q000379:methods; D025282:Mycelium / Q000235:genetics; D015203:Reproducibility of Results
chemical_list: D004271:DNA, Fungal