title: Bacterial diversity based on 16S rRNA and gyrB genes at Yinshan mine, China.
abstract: The diversity of bacterial communities at three sites impacted by acid mine drainage (AMD) from the Yinshan Mine in China was studied using comparative sequence analysis of two molecular markers, the 16S rRNA and gyrB genes. The phylogenetic analyses retrieved sequences from six classes of bacteria, Nitrospira, Alphaproteobacteria, Gammaproteobacteria, Deltaproteobacteria, Acidobacteria, and Actinobacteria, as well as sequences related to the plastid of the cyanobacterium Cyanidium acidocaldarium and also some unknown bacteria. The results of phylogenetic analyses based on gyrB and 16S rRNA were compared. This confirmed that gyrB gene analysis may be a useful tool, in addition to the comparative sequence analysis of the 16S rRNA gene, for the analysis of microbial community compositions. Moreover, the Mantel test showed that the geochemical characteristics, especially the pH value and the concentration of iron, strongly influenced the composition of the microbial communities.
authors.affiliation: School of Minerals Processing and Bioengineering, Central South University, Changsha, Hunan, China.
authors.forename: Huaqun
authors.initials: H
authors.lastname: Yin
authors.forename: Linhui
authors.initials: L
authors.lastname: Cao
authors.forename: Ming
authors.initials: M
authors.lastname: Xie
authors.forename: Qijiong
authors.initials: Q
authors.lastname: Chen
authors.forename: Guanzhou
authors.initials: G
authors.lastname: Qiu
authors.forename: Jizhong
authors.initials: J
authors.lastname: Zhou
authors.forename: Liyou
authors.initials: L
authors.lastname: Wu
authors.forename: Dianzuo
authors.initials: D
authors.lastname: Wang
authors.forename: Xueduan
authors.initials: X
authors.lastname: Liu
mesh_terms: D039903:Actinobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D020561:Alphaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics; D044822:Biodiversity; D002681:China; D027081:DNA Gyrase / Q000235:genetics; D020564:Deltaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D004323:Drainage, Sanitary; D004777:Environment; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D008906:Mining; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length; D020560:Proteobacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014871:Water Microbiology
chemical_list: D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D027081:DNA Gyrase