A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans.
Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression investigations, protein-based validation is less developed. This report illustrates the potential for two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-DE) and gel analysis to quantify protein levels following RNAi. This case study involves three glutathione transferase (GST) genes targeted by RNAi from the model organism Caenorhabditis elegans.
Lacourse
E J
EJ
School of Biological Sciences, Biosciences Building, The University of Liverpool, Liverpool, UK. james.la-course@liverpool.ac.uk
Perally
S
S
Hernandez-Viadel
M
M
Wright
H A
HA
Brophy
P M
PM
D000818:Animals; D017173:Caenorhabditis elegans / Q000201:enzymology* / Q000235:genetics; D015180:Electrophoresis, Gel, Two-Dimensional; D005982:Glutathione Transferase / Q000378:metabolism*; D015801:Helminth Proteins / Q000378:metabolism*; D040901:Proteomics; D034622:RNA Interference; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D015801:Helminth Proteins; D005982:Glutathione Transferase