title: Identification and characterization of a long isoform of human IFT80, IFT80-L.
abstract: Intraflagellar transport (IFT) proteins are evolutionarily conserved throughout all ciliated organisms and are essential for the assembly and maintenance of cilia. IFT80, a component of the IFT complex, was linked recently to a human developmental disorder, Jeune asphyxiating thoracic dystrophy. We report here identification and characterization of a human IFT80 long isoform (namely IFT80-L), the carboxyl terminus of which shares the protein sequence of IFT80. Sequence analysis indicates that IFT80-L is likely an evolutionarily merged product of genes IFT80 and TRIM59, a RING finger gene we reported previously. Expression analysis of IFT80-L demonstrates that IFT80-L is ubiquitously expressed in humans. By using the nerve growth factor-induced cell differentiation assays, we reveal that IFT80-L is highly expressed in the rapidly proliferating cells but not in differentiated cells, which withdraw from the cell cycle. Our findings suggest that IFT80-L, like other IFT proteins, plays an important role in cell proliferation and differentiation.
authors.affiliation: Department of Psychiatry and Neurobehavioral Sciences, University of Virginia, Charlottesville, VA 22911, USA.
authors.forename: Weihua
authors.initials: W
authors.lastname: Huang
authors.forename: Justin K
authors.initials: JK
authors.lastname: Kane
authors.forename: Ming D
authors.initials: MD
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism; D002453:Cell Cycle; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D049109:Cell Proliferation; D017124:Conserved Sequence; D019143:Evolution, Molecular; D015870:Gene Expression; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D008667:Metalloproteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008969:Molecular Sequence Data; D020932:Nerve Growth Factor / Q000494:pharmacology; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D017434:Protein Structure, Tertiary; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D000070584:Tripartite Motif Proteins
chemical_list: D002352:Carrier Proteins; C530863:IFT80 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins; D008667:Metalloproteins; D020033:Protein Isoforms; D034741:RNA, Small Interfering; C530864:TRIM59 protein, human; D000070584:Tripartite Motif Proteins; D020932:Nerve Growth Factor