title: Selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation: an in vitro study.
abstract: This work describes the selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation. We observed: (1) single pulsed laser irradiation caused cellular damages on pigmented, and not on non-pigmented RPE cells at laser radiant exposure up to 2550 mJ/cm(2); (2) in the mixture of pigmented and non-pigmented RPE cells, single pulsed laser-induced damage was confined to pigmented RPE cells. This study demonstrates that the pigmented RPE cells can be selectively damaged, using a single pulsed laser irradiation, without thermal coagulation to adjacent non-pigmented RPE cells.
authors.affiliation: The Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School and ShrinersBurns Hospital, 51 Blossom Street, Boston, MA 02114, USA.
authors.forename: Q
authors.initials: Q
authors.lastname: Song
authors.forename: R
authors.initials: R
authors.lastname: Risco
authors.forename: M
authors.initials: M
authors.lastname: Latina
authors.forename: F
authors.initials: F
authors.lastname: Berthiaume
authors.forename: Y
authors.initials: Y
authors.lastname: Nahmias
authors.forename: M L
authors.initials: ML
authors.lastname: Yarmush
mesh_terms: D001709:Biotechnology / Q000379:methods; D002462:Cell Membrane / Q000378:metabolism; D002470:Cell Survival; D002478:Cells, Cultured; D004867:Equipment Design; D006801:Humans; D066298:In Vitro Techniques; D007834:Lasers; D008543:Melanins / Q000378:metabolism; D008954:Models, Biological; D055095:Optics and Photonics; D010587:Phagocytosis; D010857:Pigment Epithelium of Eye / Q000166:cytology* / Q000528:radiation effects*; D012160:Retina / Q000528:radiation effects; D013053:Spectrophotometry / Q000379:methods
chemical_list: D008543:Melanins