In vitro maturation and fertilization of oocytes from unstimulated ovaries in infertile women with polycystic ovary syndrome.
OBJECTIVE
To evaluate the effects of in vitro maturation and fertilization of oocytes from unstimulated ovaries in infertile women with polycystic ovary syndrome (PCOS).


METHODS
Retrospective study.


METHODS
Reproductive Medicine Center, First Affiliated Hospital of Wenzhou Medical College, People's Republic of China.


METHODS
One hundred eighteen women with PCOS undergoing 152 cycles of in vitro maturation treatment.


METHODS
Oocyte retrieval was carried out by ultrasound-guided puncture on days 9-14 of the cycle. The oocytes were cultured in vitro using maturation culture medium, which consisted of M-199 + 20% fetal bovine serum (FBS) + 75 mIU/mL recombinant FSH +/- 0.5 IU/mL hCG. After the oocytes had matured in vitro, fertilization and embryo transfer were performed.


METHODS
Rates of clinical pregnancy, multiple pregnancies, and live birth.


RESULTS
Relatively optimal laboratory results were obtained in this study. Embryo transfer was performed in 140 cycles, with a clinical pregnancy rate (PR) of 40.0% per transfer. Fifty-six babies have been born and there are 10 ongoing pregnancies. The overall multiple PR was 33.93%.


CONCLUSIONS
Our results show that using in vitro matured oocytes from unstimulated ovaries could be offered as an alternative to conventional IVF in women with PCOS, and future work should address ways to decrease the incidence of multiple pregnancies.
Zhao
Jun-Zhao
JZ
Reproductive Medicine Center, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, People's Republic of China. joyce671128@163.com
Zhou
Wei
W
Zhang
Wei
W
Ge
Hong-Shan
HS
Huang
Xue-Feng
XF
Lin
Jin-Ju
JJ
D000328:Adult; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000503:physiopathology*; D050498:Live Birth / Q000453:epidemiology; D007986:Luteinizing Hormone / Q000097:blood; D054315:Oocyte Retrieval / Q000379:methods*; D009865:Oocytes / Q000502:physiology*; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000503:physiopathology*; D011247:Pregnancy; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic
D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone