title: Effects of hyperbaric oxygen therapy on tumor growth in murine model of PC-3 prostate cancer cell line.
abstract: OBJECTIVE
  To test the hypothesis that hyperbaric oxygen (HBO) has no effect on tumor growth in a murine model of indolent in vivo prostate cancer. HBO means breathing pure (100%) oxygen under increased atmospheric pressure.
  
  
  METHODS
  Human prostate PC-3 cells were injected into 40 severe combined-immunodeficient mice. They were randomized to undergo 20 sessions of either HBO or normobaric air in standardized conditions and observed for 4 weeks before histologic assessment of any palpable tumors that had developed. The analysis of the developed PC-3 tumors included tumor volume, microvessel density, apoptosis-associated markers (ie, p53, p27), and the proliferative index (Ki-67).
  
  
  RESULTS
  The exposure to HBO at 2 atm for 20 treatment sessions, which comprised a daily 90-minute session, 5 d/wk, had no effect on the prostate cancer volume (P > .05). No differences were observed in tumor microvessel density, proliferative index, or apoptosis markers compared with the non-HBO group (P > .05).
  
  
  CONCLUSIONS
  HBO did not have a tumor stimulatory effect on prostate cancer and could potentially be used safely in conjunction with other therapeutic modalities.
authors.affiliation: Department of Urology, Shanghai Changhai Hospital, Shanghai, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Sun
authors.forename: Chuanliang
authors.initials: C
authors.lastname: Xu
authors.forename: Tie
authors.initials: T
authors.lastname: Zhou
authors.forename: Xu
authors.initials: X
authors.lastname: Gao
authors.forename: Linhui
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000473:pathology*