[Theoretical and experimental studies on solubility parameters of multiple components for traditional Chinese materia medica with HPLC].
OBJECTIVE
To establish the theories and methods to determine solubility parameters of multiple components for the traditional Chinese material medica (TCMM) with HPLC.


METHODS
The mathematical expresses to determin the solubility parameters were established according to chromatographic and Hildebrand-Scatchard theories, The HPLC experiments were carried out at 40 degrees C on an Alltech Apollo C18 column (4.6 mm x 250 mm, 5 microm) eluted with acetone and water in gradient mode. The flow rate was 1.0 mL min(-1), and the detection wavelength was 254 nm. The injection volume was 1 0 microL.


RESULTS
The mathematical expresses between the retention time and the solubility parameters were established and used to determin caffeine solubility parameter as 28.31 J(1/2) cm(-3/2) in accordance with 28.84 J(1/2) cm(-3/2) reported by literature, and those of aloe-emodin, rhein, emodin, physcione as 39.70 J(1/2) cm(-3/2), 39.08 J(1/2) cm(-3/2), 38.37 J(1/2) cm(-3/2), 36.42 J(1/2) cm(-3/2) respectively.


CONCLUSIONS
The retention time of HPLC can be used to determine the solubility parameters of multiple componets. The established method is useful for the compatibility rule study of traditional Chinese medicine.
He
Fu-Yuan
FY
Pharmacogenetic Research Institute, Central South University, Changsha 410078, China. pharmsharking@tom.com
Zhou
Hong-Hao
HH
Luo
Jie-Ying
JY
Deng
Kai-Wen
KW
Fu
Jin
J
Pi
Feng-Juan
FJ
Wu
De-Zhi
DZ
Dai
Ru-Wen
RW
D000880:Anthraquinones / Q000737:chemistry; D002110:Caffeine / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004642:Emodin / Q000031:analogs & derivatives / Q000737:chemistry; D008419:Materia Medica / Q000737:chemistry*; D008956:Models, Chemical; D012044:Regression Analysis; D012995:Solubility; D013696:Temperature
D000880:Anthraquinones; D004365:Drugs, Chinese Herbal; D008419:Materia Medica; D002110:Caffeine; C008905:physcione; D004642:Emodin; C020491:rhein