Multixenobiotic resistance, acetyl-choline esterase activity and total oxyradical scavenging capacity of the Arctic spider crab, Hyasaraneus, following exposure to bisphenol A, tetra bromo diphenyl ether and diallyl phthalate.
The Arctic has become a sink for persistent organic pollutants (POPs) originating from lower latitudes, and relatively high levels have been found in different biota. Recent studies have identified detrimental effects on wildlife including endocrine disruption, impairment of enzyme activity, and reduced immune function. The Arctic spider crab, Hyas araneus, shown interesting potential for its use as sentinel organism in polar ecosystems. This study investigated the effect of 2,2',4,4'-tetra bromo diphenyl ether (BPDE), bisphenol A (BPA), and diallyl phthalte (DPA) on H. araneus in a three weeks exposure study. Expression of multixenibiotic resistance (MXR) proteins has been studied using the C219 monoclonal antibody which allows identifying an immunoreactive protein of 40 kDa in the digestive gland while no such protein could be observed in the gills. Expression of this protein was increased by exposure to DPA (+75%; p<0.05, n=10). All compounds significantly affected muscle acetylcholine esterase (AChE) activity (p<0.05, n=10) with 50 microg/L DPA having the strongest effect by lowering the value to 37% of control. The total oxyradical scavenging capacity measured in the digestive gland toward peroxyl, hydroxyl and peroxynitrite was also significantly reduced indicating a decreased resistance to oxidative stress generated by DPA (p<0.05, n=5). These results thus suggest the potential detrimental effects of DPA even at concentration as low as 50 microg/L on H. araneus.
Minier
Christophe
C
LEMA, EA 3222, Université du Havre, 25, rue Philippe Lebon, BP540, F-76058 Le Havre, France. minier@univ-lehavre.fr
Forget-Leray
Joëlle
J
Bjørnstad
Anne
A
Camus
Lionel
L
D000110:Acetylcholinesterase / Q000378:metabolism*; D000818:Animals; D001110:Arctic Regions; D001559:Benzhydryl Compounds; D003386:Brachyura / Q000187:drug effects* / Q000201:enzymology*; D004064:Digestive System / Q000187:drug effects; D018432:Drug Resistance, Multiple; D005786:Gene Expression Regulation / Q000187:drug effects; D055768:Halogenated Diphenyl Ethers; D006842:Hydrocarbons, Brominated / Q000494:pharmacology / Q000633:toxicity; D009132:Muscles / Q000187:drug effects / Q000201:enzymology; D010636:Phenols / Q000494:pharmacology / Q000633:toxicity; D010647:Phenyl Ethers / Q000494:pharmacology / Q000633:toxicity; D010795:Phthalic Acids / Q000633:toxicity; D011075:Polybrominated Biphenyls; D017382:Reactive Oxygen Species / Q000378:metabolism*; D014873:Water Pollutants / Q000494:pharmacology / Q000633:toxicity*; D015262:Xenobiotics / Q000494:pharmacology* / Q000633:toxicity; D000098826:Bisphenol A Compounds
D000110:Acetylcholinesterase; D001559:Benzhydryl Compounds; D055768:Halogenated Diphenyl Ethers; D006842:Hydrocarbons, Brominated; D010636:Phenols; D010647:Phenyl Ethers; D010795:Phthalic Acids; D011075:Polybrominated Biphenyls; D017382:Reactive Oxygen Species; D014873:Water Pollutants; D015262:Xenobiotics; C511295:2,2',4,4'-tetrabromodiphenyl ether; C049098:diallyl phthalate; C006780:bisphenol A; D000098826:Bisphenol A Compounds