Immunohistochemistry of pulmonary and pleural neoplasia.
BACKGROUND
The use of immunohistochemical stains has several applications in the evaluation of pulmonary and pleural neoplasms. The number of available immunohistochemical stains is continually expanding and, although these stains are an important diagnostic adjunct, their use is not without limitations and pitfalls.


OBJECTIVE
To review and summarize the primary immunohistochemical applications with regard to the most common pulmonary and pleural neoplasms and discuss newly developed markers and common pitfalls.


METHODS
Pertinent peer-reviewed literature emphasizing immunohistochemical applications in common lung and pleural tumors, unusual and problematic staining patterns in frequently encountered subtypes/histologic variants, and recently developed immunohistochemical stains of potential promise.


CONCLUSIONS
Immunohistochemical stains provide the greatest aid in establishing the site of origin of adenocarcinomas encountered in the lung and in separating epithelioid mesothelioma from adenocarcinoma. Certain subtypes of pulmonary adenocarcinomas may exhibit unusual staining patterns that may be potentially problematic. Immunohistochemistry has a more limited role in separating small cell carcinoma from non-small cell carcinoma, and extreme caution must be used in the situation of crushed biopsy specimens.
Beasley
Mary Beth
MB
Department of Pathology, Mount Sinai Medical Center, New York, NY 10029, USA. mbbeasleymd@yahoo.com
D014408:Biomarkers, Tumor / Q000032:analysis; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D010997:Pleural Neoplasms / Q000378:metabolism* / Q000473:pathology
D014408:Biomarkers, Tumor