title: Kinetic models for enzymic cellulose degradation in aqueous two-phase systems.
abstract: Cellulose materials can readily be degraded into cellobiose and glucose by hydrolysis of the enzymes cellulase and beta-glucosidase in aqueous media. Product inhibition does, however, retard the reaction rate and reduce productivity. This may be avoided by carrying out the degradation of cellulose in an aqueous two-phase system, which permits the enzymes and the substrate to be partitioned to one phase and the products to be extracted into a second phase. In addition, two-phase systems also allow recycling of the enzymes. Here, three models previously developed for "one-phase" enzymic degradation are compared to data from enzymic degradation in an aqueous two-phase system. The models tested agreed relatively well with batch experiments during a period of 200 h. For one of the models tested, continuous degradation also gave accurate agreement.
authors.affiliation: Pure and Applied Biochemistry, University of Lund, Box 124, S-221 00 Lund, Sweden.
authors.forename: C F
authors.initials: CF
authors.lastname: Mandenius
authors.forename: B
authors.initials: B
authors.lastname: Nilsson
authors.forename: I
authors.initials: I
authors.lastname: Persson
authors.forename: F
authors.initials: F
authors.lastname: Tjerneld