title: Differential tissue and flight developmental expression of glycerol-3-phosphate dehydrogenase isozymes in the Chagas disease vector Triatoma infestans.
abstract: Glycerol-3-phosphate dehydrogenase (GPDH) isozymes are differentially expressed among tissues and during flight development. GPDH-1 is involved in the flight-muscle metabolism and GPDH-2 provides precursors for lipid biosynthesis in many tissues. We have isolated and characterized from Triatoma infestans, a Chagas disease vector, two cDNAs encoding for GPDH-1 and GPDH-2 isozymes. The inferred amino acid sequences showed high identity with other GPDH sequences from flying insects. A GPDH-2 transcript was found in fifth instar nymphs, thoracic muscles, adult gonads, and fat bodies. Both isozymes are present in 30-day-old adult thoracic muscle transcripts, and the pattern of expression differs between sexes. The expression of GPDH-1 begins earlier in females, and GPDH-2 is expressed more abundantly in female adult thoracic muscles than in those from males. This finding is consistent with those of other investigators who showed a higher flight initiation probability in T. infestans females than in males.
authors.affiliation: Departamento de Bioquímica y Biología Molecular, Facultad de Medicina, Universidad Nacional de Córdoba, Córdoba, Argentina. mercedesstroppa@hotmail.com
authors.forename: María
authors.initials: M
authors.lastname: Mercedes Stroppa
authors.forename: Carlota
authors.initials: C
authors.lastname: Carriazo
authors.forename: Néstor
authors.initials: N
authors.lastname: Soria
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Pereira
authors.forename: Nelia M
authors.initials: NM
authors.lastname: Gerez de Burgos
mesh_terms: D000818:Animals; D014355:Chagas Disease / Q000469:parasitology; D004199:Disease Vectors; D005426:Flight, Animal / Q000502:physiology*; D015870:Gene Expression; D005993:Glycerolphosphate Dehydrogenase / Q000096:biosynthesis*; D007303:Insect Vectors / Q000201:enzymology* / Q000254:growth & development; D007527:Isoenzymes / Q000096:biosynthesis*; D014224:Triatoma / Q000196:embryology* / Q000201:enzymology* / Q000254:growth & development
chemical_list: D007527:Isoenzymes; D005993:Glycerolphosphate Dehydrogenase