Subculture on potato dextrose agar as a complement to the broth microdilution assay for Malassezia pachydermatis.
The main aim of this study was to verify the efficacy of subculture on potato dextrose agar (PDA) as a complement to the in vitro susceptibility test for Malassezia pachydermatis strains by a broth microdilution method, as well as to determine the MIC and MFC of azole derivatives, amphotericin B and caspofungin. The microdilution assay was performed in 96-well plates using a modified RPMI 1640 medium. The M. pachydermatis strains were resistant to caspofungin. All strains (n=50) had shown MIC values of <0.03, <0.03, 2.0, 4.0 and 4.0 microg/ml for itraconazole, ketoconazole, voriconazole, fluconazole and amphotericin B, respectively. Thus, the subculture on PDA improved the analysis of the in vitro antifungal susceptibility of M. pachydermatis.
Prado
Marilena R
MR
Faculty of Veterinary Medicine, Program of Post-Graduation in Veterinary Science, State University of Ceará, Fortaleza, Ceará, Brazil. lena@inteq.com.br
Brito
Erika H S
EH
Brilhante
Raimunda S N
RS
Cordeiro
Rossana A
RA
Leite
João J G
JJ
Sidrim
José J C
JJ
Rocha
Marcos F G
MF
D000362:Agar; D000666:Amphotericin B / Q000494:pharmacology; D000818:Animals; D000935:Antifungal Agents / Q000494:pharmacology*; D001393:Azoles / Q000494:pharmacology; D000077336:Caspofungin; D003470:Culture Media; D003881:Dermatomycoses / Q000382:microbiology / Q000662:veterinary; D004283:Dog Diseases / Q000382:microbiology; D004285:Dogs; D054714:Echinocandins / Q000494:pharmacology; D005947:Glucose; D055666:Lipopeptides; D008290:Malassezia / Q000187:drug effects* / Q000254:growth & development* / Q000302:isolation & purification; D008826:Microbial Sensitivity Tests; D008828:Microbiological Techniques; D011198:Solanum tuberosum
D000935:Antifungal Agents; D001393:Azoles; D003470:Culture Media; D054714:Echinocandins; D055666:Lipopeptides; D000666:Amphotericin B; D000362:Agar; D000077336:Caspofungin; D005947:Glucose