[A prospective auto-controlled study to evaluate the interest of selected sperm morphology to inseminate oocytes during IVF].
OBJECTIVE
Since we observed in our laboratory a significant decrease of the fertilization rate in the classical IVF procedure compared to ICSI, we wanted to evaluate a new insemination procedure, using sperm morphology of selected semen as a criterion taken into account to calculate the number of mobile sperm to inseminate.


METHODS
Therefore, we designed a prospective autocontrolled study which took place from September 2006 to May 2007. All IVF attempts resulting from infertile couples were included in the study if a previous analysis (lesser than one year) of sperm parameters performed in our laboratory was assessed as normal. The retrieved oocytes were separated into two groups (sibling ovocytes), inseminated randomly either with the usual procedure (control group) or with the tested protocol (tested group). Fertilization rate was the primary end point to evaluate this assay. Polyspermy rates were also compared. Subsequently, embryonic quality and development were assessed to eliminate an oxidative stress impact. Paired Student t-test was applied for statistical analysis.


RESULTS
In all, 130 couples were included. The diploid fertilization rate was significantly increased in the tested group compared to the control group (66.9% vs 61.3%; p=0.017). No statistically significant difference was showed between the polyspermy rate and numbers of (i) good quality embryos and (ii) blastomeres per embryo into the two groups.


CONCLUSIONS
Compared with the conventional insemination procedure, the fertilization rate benefited from the tested insemination method in our laboratory without damaging embryonic growth. In the limit of our procedure, selected sperm morphology should be used to inseminate an appropriate number of spermatozoa within oocytes, even in case of normal semen values.
Grange
E
E
Service d'histologie-embryologie-cytogénétique, hôpital Jean-Verdier, assistance publique-Hôpitaux de Paris (AP-HP), avenue du 14-Juillet, Bondy cedex, France.
Le Teuff
G
G
Martin-Pont
B
B
Wolf
J-P
JP
Sifer
C
C
D010064:Embryo Implantation / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D007315:Insemination, Artificial / Q000379:methods*; D008297:Male; D054315:Oocyte Retrieval; D009865:Oocytes / Q000166:cytology* / Q000502:physiology*; D011247:Pregnancy; D011446:Prospective Studies; D035781:Siblings; D013094:Spermatozoa / Q000166:cytology* / Q000502:physiology*