Secondary disease mortality in rat-mouse radiation chimeras.
Two statistical experimental designs were used to investigate factors involved in 90-day mortality from secondary disease in lethally irradiated mice treated with rat bone marrow. Secondary disease is a graft-versus-host syndrome that has a mortality of about 65-95% in this transplant situation. When the factors--age-of-donor-cells, day-of-cell-injection, dose-of-marrow-cells, sex, and environment--were examined for their main effects and interactions, some combinations of these factors were found to give about 25% 90-day mortality. The experiments indicate that the lowest mortality can be achieved with an injection of 40 million or more cells 1 day after irradiation and an ultraclean environment of unlimited filter-top caging.
Congdon
C C
CC
Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee 37830, USA.
Mitchell
T J
TJ
Gardiner
D A
DA
Kastenbaum
M A
MA
Toya
R E
RE
D000367:Age Factors; D000818:Animals; D016026:Bone Marrow Transplantation; D004780:Environment, Controlled; D005260:Female; D006086:Graft vs Host Disease / Q000401:mortality*; D008297:Male; D051379:Mice; D015233:Models, Statistical; D011828:Radiation Chimera; D051381:Rats; D012737:Sex Factors; D013997:Time Factors; D014916:Whole-Body Irradiation / Q000401:mortality