Cloning and characterization of the gene cluster for biosynthesis of ectoine from Nesterenkonia halobia DSM 20541.
The ectABC genes encoding the biosynthesis of ectoine were identified from Nesterenkonia halobia DSM 20541. The intergenic regions of the ectABC genes from N. halobia DSM 20541 were more loosely spaced than those that had been reported before. The amino acid sequence deduced from ectABC of the strain was highly homologous to the EctABC of Brevibacterium linens BL2 (EctA 50%, EctB 70%, and EctC 68% identities). The osmoprotection of ectABC was studied in the Escherichia coli KNabc and E. coli XL1-Blue. The results revealed that ectABC could shorten the lag phase and enhance the final OD600 of E. coli XL1-Blue in MM63 medium containing 0.68 M NaCl, and could initiate KNabc growth in 0.2 M NaCl. Ectoine was proven to be accumulated in E. coli KNabc/pGEM-Nect using HPLC-UV, and validated by LC-MSD-Trap-VL.
Zhang
Bo
B
Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
Bao
Xin
X
Wang
Lei
L
Yang
Su Sheng
SS
D000595:Amino Acid Sequence; D000599:Amino Acids, Diamino / Q000096:biosynthesis* / Q000235:genetics; D001419:Bacteria / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002712:Chlorides / Q000378:metabolism; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D008835:Micrococcaceae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D005810:Multigene Family; D009997:Osmotic Pressure; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid
D000599:Amino Acids, Diamino; D001426:Bacterial Proteins; D002712:Chlorides; C045628:ectoine; C001599:chlorite