Lactase production by solid-state cultivation of Kluyveromyces marxianus CDBBL 278 on an inert support: effect of inoculum, buffer, and nitrogen source.
A study was carried out to select the conditions for cultivation of Kluyveromyces marxianus CDBBL 278 in solid-state culture (SSC) using polyurethane foam (PUF) as an inert support. PUF was impregnated with culture media containing lactose (50 g/L) as the carbon and energy source. Evaluation of culture parameters during different growth phases was carried out by respirometry. The effect of inoculum level, buffer capacity of the medium, and nitrogen source upon the yield of biomass on lactose (Yx/s) and production of lactase and inulinase was investigated. The highest lactase titre was achieved with an inoculum level of 1 x 10(7) cells per gram of wet matter (gwm) and 20% of the total nitrogen source provided as urea. The best biomass yield (0.37) was obtained when less than 40% of the total nitrogen was provided as urea. Using potassium phosphate allowed 90% substrate consumption in 30 h. In the best conditions, intracellular lactase and extracellular inulinase activities of 1147.7 IU/gX and 241.6 IU/gX were obtained, respectively, with a lag phase of 13.8 h and a rate of respiratory activity (microCO2) of 0.23 +/- 0.01 h(-1). To our knowledge, this is the first report on lactase production by K. marxianus CDBBL 278 in SSC. This study gives basic information about biomass yield and enzyme production using lactose as the sole carbon source in SSC on an inert support.
Tovar-Castro
Luz
L
Departamento de Biotecnología, Universidad Autónoma Metropolitana Iztapalapa, San Rafael Atlixco, Delegación Iztapalapa, México.
García-Garibay
Mariano
M
Saucedo-Castañeda
Gerardo
G
D002021:Buffers; D003470:Culture Media; D007716:Kluyveromyces / Q000254:growth & development / Q000378:metabolism*; D043322:Lactase / Q000096:biosynthesis*; D007785:Lactose / Q000378:metabolism; D009172:Mycology / Q000379:methods; D009584:Nitrogen / Q000008:administration & dosage; D011140:Polyurethanes
D002021:Buffers; D003470:Culture Media; D011140:Polyurethanes; C028279:polyurethane foam; D043322:Lactase; D007785:Lactose; D009584:Nitrogen