title: Partitioning of host and recombinant cells in aqueous two-phase polymer systems.
abstract: The separation of host and recombinant Escherichia coli bacterial cells has been studied using the surface-sensitive technique of partitioning in aqueous two-phase polymer systems. Experiments were designed to probe charge-and hydrophobicity-related property differences of antibiotic-resistant recombinant cells and their antibiotic-sensitive hosts. Differential partitioning was observed in both charge-sensitive and non-charge-sensitive phase systems for three host-recombinant cell systems, but the non-charge-related effects appear to have a greater impact on partitioning behavior. This result suggests that plasmid-encoded products related to antibiotic resistance modify the surface hydrophobicity of the E. coli bacterial cell and that these differences can be exploited for cell separation.
authors.affiliation: Department of Chemical Engineering, University of Delaware, Newark, Delaware 19716, USA.
authors.forename: C
authors.initials: C
authors.lastname: LaMarca
authors.forename: A M
authors.initials: AM
authors.lastname: Lenhoff
authors.forename: P
authors.initials: P
authors.lastname: Dhurjati