Apolipoprotein E polymorphism in normal Han Chinese population: frequency and effect on lipid parameters.
Apolipoprotein E (ApoE) genotypes were studied in order to determine the prevalence and effect on lipid parameters in normal Han Chinese population. Fragments of ApoE gene forth exon containing codon 112 and 158 polymorphic locus were amplified by PCR, and then digested with Cfo I endonuclease. Genotypes and alleles frequencies of 168 healthy Han Chinese were calculated. The frequency of genotypes epsilon3/3, epsilon3/4, and epsilon2/3 was found to be 75.00, 10.70, and 11.90%, respectively, and 0.60, 1.20, and 0.60% for epsilon2/2, epsilon2/4, and epsilon4/4. The effects of ApoE genotypes and alleles on lipid parameters were analyzed. The effects of ApoE alleles on TC, LDL-C, ApoB was: along a decreasing gradient epsilon4 > epsilon3 > epsilon2. The effect of epsilon4 allele was to increase serum levels of TC, LDL-C and ApoB, and epsilon2 allele had an effect opposite to that of epsilon4 allele. Results obtained in this study indicate that ApoE polymorphism is an independent genetic factor on individual serum levels of lipids and apolipoproteins.
Liang
Shu
S
Department of Neurology, Second Affiliated Hospital of Soochow University, 1055 Sanxiang Road, Suzhou, 215000, People's Republic of China.
Pan
Min
M
Geng
Hai-Hua
HH
Chen
Hui
H
Gu
Li-Qun
LQ
Qin
Xiao-Tong
XT
Qian
Jin-Jun
JJ
Zhu
Jian-Hua
JH
Liu
Chun-Feng
CF
D001053:Apolipoproteins / Q000097:blood; D001055:Apolipoproteins B / Q000097:blood; D001057:Apolipoproteins E / Q000235:genetics*; D002681:China / Q000453:epidemiology; D002784:Cholesterol / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D005091:Exons; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008055:Lipids / Q000097:blood*; D011110:Polymorphism, Genetic / Q000235:genetics*
D001053:Apolipoproteins; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D008078:Cholesterol, LDL; D008055:Lipids; D002784:Cholesterol