Human embryonic stem cell-derived oligodendrocyte progenitor cells express the serotonin receptor and are susceptible to JC virus infection.
We studied the susceptibility of human embryonic stem cell-derived oligodendrocyte progenitor cells to infection with JC virus, the causative agent of progressive multifocal leukoencephalopathy (PML). A human embryonic stem cell line, H7, was used to derive an enriched population of cells expressing the oligodendrocyte progenitor cell-specific marker NG2. These cells expressed the 5HT2a receptor (5HT2aR) for JC virus and were highly susceptible to infection. Infection was reduced by treatment with anti-5HT2aR antibodies and by the 5HT2aR antagonists ritanserin and ketanserin. This is the first demonstration that human embryonic stem cell-derived oligodendrocyte progenitor cells are susceptible to JC virus infection and indicates that cells poised to replenish mature oligodendrocytes in PML lesions may also be a target of viral infection.
Schaumburg
C
C
Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA.
O'Hara
B A
BA
Lane
T E
TE
Atwood
W J
WJ
D000911:Antibodies, Monoclonal; D000941:Antigens / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002460:Cell Line; D053595:Embryonic Stem Cells / Q000166:cytology*; D005333:Fetus / Q000166:cytology; D006801:Humans; D007577:JC Virus / Q000502:physiology*; D007650:Ketanserin / Q000494:pharmacology; D007968:Leukoencephalopathy, Progressive Multifocal / Q000235:genetics / Q000473:pathology* / Q000821:virology*; D009836:Oligodendroglia / Q000821:virology*; D011509:Proteoglycans / Q000378:metabolism; D044402:Receptor, Serotonin, 5-HT2A / Q000276:immunology / Q000502:physiology*; D016713:Ritanserin / Q000494:pharmacology; D058830:Serotonin 5-HT2 Receptor Antagonists; D012702:Serotonin Antagonists / Q000494:pharmacology; D000099216:Chondroitin Sulfate Proteoglycan 4
D000911:Antibodies, Monoclonal; D000941:Antigens; D015415:Biomarkers; D007650:Ketanserin; D011509:Proteoglycans; D044402:Receptor, Serotonin, 5-HT2A; D016713:Ritanserin; D058830:Serotonin 5-HT2 Receptor Antagonists; D012702:Serotonin Antagonists; D000099216:Chondroitin Sulfate Proteoglycan 4