title: Quantitative determination of cyclic polylactic acid oligomers in serum by direct injection liquid chromatography tandem mass spectrometry.
abstract: Polylactic acid (PLA) is a biodegradable polymer, currently used in pharmaceutical and surgical devices. There is a concern that cyclic polylactic acid (CPLA), which is a by-product of PLA synthesis, may be introduced into the human body as an undesirable contaminant. We carried out a quantitation investigation of the CPLA heptamer (CPLA-7) by liquid chromatography mass spectrometry (LC-MS). We found that CPLA-7 binds strongly with serum proteins and that only 62% of CPLA-7 was recovered after routine deproteination; therefore, we directly injected serum into the LC-MS/MS system after passage through a bovine serum albumin (BSA)-coated chromatographic column and found the recovery of CPLA-7 was improved to 84%, and that the detection (S/N=3) and quantitation limit (S/N=10 and below 15% relative standard deviation) were 1.5 and 2.5 ng/mL, respectively. We conclude that direct injection LC-MS/MS, using a BSA column, is a simple and effective quantitative analysis method for CPLA in serum.
authors.affiliation: Department of Applied Chemistry, Kansai University, 3-3-35 Yamatecho, Suita, Osaka 564-8680, Japan.
authors.forename: Issey
authors.initials: I
authors.lastname: Osaka
authors.forename: Arihumi
authors.initials: A
authors.lastname: Yoshimoto
authors.forename: Mikio
authors.initials: M
authors.lastname: Watanabe
authors.forename: Masashi
authors.initials: M
authors.lastname: Takama
authors.forename: Masahiro
authors.initials: M
authors.lastname: Murakami
authors.forename: Hideya
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Arakawa
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D006801:Humans; D019344:Lactic Acid / Q000097:blood* / Q000737:chemistry; D011091:Polyesters; D011108:Polymers / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011091:Polyesters; D011108:Polymers; D019344:Lactic Acid; C033616:poly(lactide)