title: Inhibitory action of palatinose and its hydrogenated derivatives on the hydrolysis of alpha-glucosylsaccharides in the small intestine.
abstract: This study was conducted to investigate the inhibitory effects of palatinose and Palatinit, which are disaccharides (or disaccharide alcohol) connected through an alpha-1,6-glucosyl linkage, on the hydrolysis of other carbohydrates, using an enzyme extract from the rat small intestine and a purified sucrase-isomaltase complex. Palatinose and its hydrogenated product, Palatinit, an equimolar mixture of alpha-O-D-glucopyranosyl-1,6-D-sorbitol (GPS) and alpha-O-D-glucopyranosyl-1,6-D-mannitol (GPM), inhibited the hydrolysis of sucrose and maltose. Palatinose and Palatinit also inhibited the hydrolysis of dextrin and soluble starch. Kinetic analysis of the enzymatic inhibition by GPS and GPM on sucrose hydrolysis revealed that both GPS and GPM competitively inhibit sucrase catalytic activity. These results suggest that disaccharides with an alpha-1,6-glucosyl linkage competitively inhibit intestinal alpha-glucosidases and may reduce the rate of hydrolysis of sucrose and other alpha-glucosylsaccharides.
authors.affiliation: Research Laboratory, Mitsui Sugar Co., Ltd., 1-2-14, Honson, Chigasaki-shi, Kanagawa, 253-0042 Japan. jun.kashimura@mitsui-sugar.co.jp
authors.forename: Jun
authors.initials: J
authors.lastname: Kashimura
authors.forename: Yukie
authors.initials: Y
authors.lastname: Nagai
authors.forename: Toshinao
authors.initials: T
authors.lastname: Goda
mesh_terms: D002236:Carbohydrate Conformation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D065089:Glycoside Hydrolase Inhibitors; D006865:Hydrogenation; D006868:Hydrolysis; D007421:Intestine, Small / Q000201:enzymology*; D007534:Isomaltose / Q000031:analogs & derivatives* / Q000494:pharmacology; D008320:Maltose / Q000378:metabolism*; D013394:Sucrase-Isomaltase Complex / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism*; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D013395:Sucrose; D007534:Isomaltose; D008320:Maltose; D013394:Sucrase-Isomaltase Complex; D000520:alpha-Glucosidases; C008189:isomaltulose