Robiginitalea myxolifaciens sp. nov., a novel myxol-producing bacterium isolated from marine sediment, and emended description of the genus Robiginitalea.
A marine, Gram-negative, deep-orange-coloured, rod/coccoid-shaped, strictly aerobic, non-motile, oxidase- and catalase-positive bacterial strain, designated YM6-073(T), was isolated from sediment collected in Okinawa, Japan. Strain YM6-073(T) produced the carotenoid (3R,2'S)-myxol. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YM6-073(T) belonged to a distinct lineage in the family Flavobacteriaceae, the closest relative being Robiginitalea biformata HTCC2501(T) (93.7 % sequence similarity). Strain YM6-073(T) could be distinguished from other members of the family Flavobacteriaceae by means of a number of phenotypic characteristics. The DNA G+C content was 50.1 mol%. The predominant cellular fatty acids were iso-C(15 : 0), iso-C(15 : 1)omega10c, iso-C(17 : 0) 3-OH and C(15 : 0,) and menaquinone-6 was the only respiratory quinone. DNA-DNA hybridization between strain YM6-073(T) and R. biformata DSM 15991(T) showed less than 10 % DNA-DNA relatedness. On the basis of phenotypic, genotypic and phylogenetic features, strain YM6-073(T) represents a novel species of the genus Robiginitalea, for which the name Robiginitalea myxolifaciens sp. nov. is proposed. The type strain is YM6-073(T) (=MBIC 06409(T)=KCTC 22216(T)=DSM 21019(T)).
Manh
Hao Do
HD
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Matsuo
Yoshihide
Y
Katsuta
Atsuko
A
Matsuda
Satoru
S
Shizuri
Yoshikazu
Y
Kasai
Hiroaki
H
D002338:Carotenoids / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D041965:Flavobacteriaceae / Q000737:chemistry / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D019015:Geologic Sediments / Q000382:microbiology*; D007564:Japan; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; C488895:myxol; D002338:Carotenoids