title: Using peripheral blood mononuclear cells to determine proteome profiles in human cardiac failure.
abstract: BACKGROUND
  In chronic heart failure (CHF), peripheral blood mononuclear cells (PBMC) might undergo structural and/or functional alterations as a consequence of the development and progression of the disease.
  
  
  OBJECTIVE
  This study was aimed at: (1) assessing the proteome profile of PBMC from Controls and CHF subjects, (2) identifying differentially-expressed proteins in healthy subjects and patients, and (3) analysing the expression of these proteins in patients after heart transplantation.
  
  
  RESULTS
  Proteome changes were assessed in PBMC from 8 healthy and 11 end-stage CHF (6 Ischaemic Heart Failure [IHF], 5 Dilated CardioMyopathy [DCM]) subjects by gel electrophoresis, PD-Quest analysis and mass spectrometry. Eighteen proteins were differentially expressed in Controls and CHF patients. However, among CHF patients, these proteins were equally expressed in IHF and DCM subjects. Eleven proteins were found to belong to 4 functional classes (3 cytoskeletal, 4 cell-cycle progression, 2 stress response and DNA repair, 2 energetic metabolism proteins). Changes in three of the differentially-expressed proteins were also confirmed by Western blot and were reversed after heart transplantation.
  
  
  CONCLUSIONS
  Results demonstrate an altered protein expression profile in PBMC of CHF patients compared to Controls, thus providing a basis for further diagnostic and prognostic tests for CHF.
authors.affiliation: Department of Pharmacological Sciences, University of Milan, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Mazzola
authors.forename: Riccardo
authors.initials: R
authors.lastname: Cianti
authors.forename: Luca
authors.initials: L
authors.lastname: Bini
authors.forename: Alessandro
authors.initials: A
authors.lastname: Armini
authors.forename: Ivano
authors.initials: I
authors.lastname: Eberini
authors.forename: Gerarda
authors.initials: G
authors.lastname: Pompella
authors.forename: Pier Leopoldo
authors.initials: PL
authors.lastname: Capecchi
authors.forename: Mariarita
authors.initials: M
authors.lastname: Natale
authors.forename: Maria P
authors.initials: MP
authors.lastname: Abbracchio
authors.forename: Franco
authors.initials: F
authors.lastname: Laghi-Pasini
mesh_terms: D001798:Blood Proteins / Q000032:analysis*; D015153:Blotting, Western; D015180:Electrophoresis, Gel, Two-Dimensional; D006333:Heart Failure / Q000097:blood* / Q000175:diagnosis; D016027:Heart Transplantation; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry*; D011379:Prognosis
chemical_list: D001798:Blood Proteins