title: Attenuation of hypoosmotic stress-induced ANP secretion via I(Cl,swell) in renal hypertensive rat atria.
abstract: Cardiac hypertrophy, an adaptive process to an increased hemodynamic overload, includes not only an increase in cell size but also qualitative changes in constituent proteins. Although swelling-activated chloride channels (I(Cl,swell)) chronically activate in hypertrophied atrial myocytes, the role of I(Cl,swell) in regulation of atrial natriuretic peptide (ANP) release is poorly understood. We investigated the effects of I(Cl,swell) on ANP release and contractility and its modification in hypertrophied rat atria. To stimulate I(Cl,swell), hypoosmotic HEPES buffered solution (0.8T, 0.7T and 0.6T) was perfused into isolated perfused beating atria. The hypoosmotic HEPES buffered solution increased ANP release as compared to isoosmotic buffered solution (1T) in an osmolarity-reduction dependent manner. Atrial contractility and extracellular fluid translocation did not change. Exposure to hypoosmotic buffer (0.8T) containing low chloride (8mM), tamoxifen or diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS) significantly attenuated hypoosmolarity-induced ANP release. The pretreatment with genistein, okdaic acid, U73122, GF109203x, and staurosporine attenuated hypoosmolarity-induced ANP release whereas orthovanadate augmented it significantly. In hypertrophied atria from renal hypertensive rats, hypoosmolarity-induced ANP release was markedly attenuated and DIDS-induced decrease in ANP release and negative inotropy were augmented as compared to sham-operated rat atria. Therefore, we suggest that I(Cl,swell) may partly participate hypoosmolarity-induced ANP release through protein tyrosine kinase and phospholipase C-protein kinase C pathway. The modification of responses of ANP release to hypoosmolarity and DIDS in hypertrophied atria may relate to changes in I(Cl,swell) activity by persistent high blood pressure.
authors.affiliation: Department of Physiology, Medical School, Institute for Medical Sciences, 2-20 Keum-Am-Dong-San, Chonbuk National University, Jeonju 561-180, Republic of Korea.
authors.forename: Guang Yi
authors.initials: GY
authors.lastname: Bai
authors.forename: Kuichang
authors.initials: K
authors.lastname: Yuan
authors.forename: Woo Hyun
authors.initials: WH
authors.lastname: Park
authors.forename: Sung Zoo
authors.initials: SZ
authors.lastname: Kim
authors.forename: Suhn Hee
authors.initials: SH
authors.lastname: Kim
mesh_terms: D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid / Q000494:pharmacology; D000818:Animals; D009320:Atrial Natriuretic Factor / Q000378:metabolism*; D006332:Cardiomegaly / Q000503:physiopathology; D018118:Chloride Channels / Q000502:physiology*; D004963:Estrenes / Q000494:pharmacology; D045604:Extracellular Fluid / Q000502:physiology; D019833:Genistein / Q000494:pharmacology; D006325:Heart Atria / Q000187:drug effects / Q000378:metabolism*; D006977:Hypertension, Renal / Q000503:physiopathology*; D007211:Indoles / Q000494:pharmacology; D008301:Maleimides / Q000494:pharmacology; D019319:Okadaic Acid / Q000494:pharmacology; D009997:Osmotic Pressure; D011760:Pyrrolidinones / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D019311:Staurosporine / Q000494:pharmacology; D013629:Tamoxifen / Q000494:pharmacology; D014638:Vanadates / Q000494:pharmacology
chemical_list: D018118:Chloride Channels; D004963:Estrenes; D007211:Indoles; D008301:Maleimides; D011760:Pyrrolidinones; D013629:Tamoxifen; C060229:1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione; D019319:Okadaic Acid; D014638:Vanadates; D009320:Atrial Natriuretic Factor; D019833:Genistein; D019311:Staurosporine; C070515:bisindolylmaleimide I; D017878:4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid