[Assessment of sensitivity of commercial test-systems for HBsAg immunodetection according to their ability to detect HBsAg-mutants of hepatitis B virus].
Comparative study of widely distributed on Russian market commercial test-systems for HBsAg detection by enzyme immunoassay was performed. Panel of serum samples containing mutant forms of HBsAg was developed for the study. It showed that only 2 out of 7 studied test-systems are able to detect mutant forms of HBsAg with the same sensitivity as "wild-type" forms of HBsAg. Test-systems based only on monoclonal antibodies did not detect HBsAg with G145R substitution in concentration 5 ng/ml. The study confirms the relevance of problem of HBsAg-mutants detection for hepatitis B immunodiagnostics.
Bazhenov
A I
AI
Konopleva
M V
MV
Fel'dsherova
A A
AA
El'gort
D A
DA
Khats
Iu S
IuS
Lapina
N E
NE
Godkov
M A
MA
Suslov
A P
AP
D006509:Hepatitis B / Q000175:diagnosis* / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000097:blood* / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D009154:Mutation; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic