title: Hypoxia- and vascular endothelial growth factor-induced stromal cell-derived factor-1alpha/CXCR4 expression in glioblastomas: one plausible explanation of Scherer's structures.
abstract: The morphological patterns of glioma cell invasion are known as the secondary structures of Scherer. In this report, we propose a biologically based mechanism for the nonrandom formation of Scherer's secondary structures based on the differential expression of stromal cell-derived factor (SDF)-1alpha and CXCR4 at the invading edge of glioblastomas. The chemokine SDF-1alpha was highly expressed in neurons, blood vessels, subpial regions, and white matter tracts that form the basis of Scherer's secondary structures. In contrast, the SDF-1alpha receptor, CXCR4, was highly expressed in invading glioma cells organized around neurons and blood vessels, in subpial regions, and along white matter tracts. Neuronal and endothelial cells exposed to vascular endothelial growth factor up-regulated the expression of SDF-1alpha. CXCR4-positive tumor cells migrated toward a SDF-1alpha gradient in vitro, whereas inhibition of CXCR4 expression decreased their migration. Similarly, inhibition of CXCR4 decreased levels of SDF-1alpha-induced phosphorylation of FAK, AKT, and ERK1/2, suggesting CXCR4 involvement in glioma invasion signaling. These studies offer one plausible molecular basis and explanation of the formation of Scherer's structures in glioma patients.
authors.affiliation: Microvascular and Molecular Neuro-oncologyLaboratory, Department of Pathology, MSB-130, New York University School of Medicine, 550 First Ave., New York, NY 10016, USA. dz4@nyu.edu
authors.forename: David
authors.initials: D
authors.lastname: Zagzag
authors.forename: Mine
authors.initials: M
authors.lastname: Esencay
authors.forename: Olga
authors.initials: O
authors.lastname: Mendez
authors.forename: Herman
authors.initials: H
authors.lastname: Yee
authors.forename: Iva
authors.initials: I
authors.lastname: Smirnova
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Huang
authors.forename: Luis
authors.initials: L
authors.lastname: Chiriboga
authors.forename: Eugene
authors.initials: E
authors.lastname: Lukyanov
authors.forename: Mengling
authors.initials: M
authors.lastname: Liu
authors.forename: Elizabeth W
authors.initials: EW
authors.lastname: Newcomb
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001932:Brain Neoplasms / Q000378:metabolism* / Q000473:pathology; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D054377:Chemokine CXCL12 / Q000378:metabolism*; D002633:Chemotaxis; D042783:Endothelial Cells / Q000378:metabolism; D004730:Endothelium, Vascular / Q000166:cytology; D005260:Female; D005909:Glioblastoma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D009474:Neurons / Q000378:metabolism; D019718:Receptors, CXCR4 / Q000502:physiology*; D015398:Signal Transduction; D042461:Vascular Endothelial Growth Factor A / Q000494:pharmacology / Q000502:physiology*
chemical_list: D054377:Chemokine CXCL12; D019718:Receptors, CXCR4; D042461:Vascular Endothelial Growth Factor A