title: Isolation, characterization and molecular cloning of new temporins from the skin of the North African ranid Pelophylax saharica.
abstract: Temporins are small antimicrobial peptides isolated from North American and Eurasian ranid frogs that are particularly active against Gram-positive bacteria. To date, no temporins have been characterized from North African frog species. We isolated three novel members of the temporin family, named temporin-1Sa (FLSGIVGMLGKLF(amide)), -1Sb (FLPIVTNLLSGLL(amide)), and -1Sc (FLSHIAGFLSNLF(amide)), from the skin of the Sahara frog Pelophylax (Rana) saharica originating from Tunisia. These temporins were identified by a combined mass spectrometry/molecular cloning approach. Temporin-1Sa was found to be highly active against Gram-positive and Gram-negative bacteria, yeasts and fungi (MIC=2-30 microM). To our knowledge, this is the first 13-residue member of the temporin family with a net charge of +2 that shows such broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC=10 microM) and Pseudomonas aeruginosa (MIC=31 microM). Moreover, temporin-1Sa displays significant antiparasitic activity (IC50 approximately 20 microM) against the promastigote and amastigote stages of Leishmania infantum.
authors.affiliation: FRE 2852, Peptidome de la Peau des Amphibiens, Université Pierre et Marie Curie/CNRS, Tour 43, 2 place Jussieu, 75251 Paris Cedex 05, France.
authors.forename: Feten
authors.initials: F
authors.lastname: Abbassi
authors.forename: Bruno
authors.initials: B
authors.lastname: Oury
authors.forename: Thierry
authors.initials: T
authors.lastname: Blasco
authors.forename: Denis
authors.initials: D
authors.lastname: Sereno
authors.forename: Gérard
authors.initials: G
authors.lastname: Bolbach
authors.forename: Pierre
authors.initials: P
authors.lastname: Nicolas
authors.forename: Khaled
authors.initials: K
authors.lastname: Hani
authors.forename: Mohamed
authors.initials: M
authors.lastname: Amiche
authors.forename: Ali
authors.initials: A
authors.lastname: Ladram
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D000935:Antifungal Agents / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000302:isolation & purification / Q000494:pharmacology; D000981:Antiprotozoal Agents / Q000494:pharmacology; D001483:Base Sequence; D002851:Chromatography, High Pressure Liquid; D003001:Cloning, Molecular; D004912:Erythrocytes / Q000187:drug effects; D053878:Hemolytic Agents / Q000494:pharmacology; D006801:Humans; D018314:Leishmania infantum / Q000187:drug effects; D008969:Molecular Sequence Data; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*; D011898:Ranidae
chemical_list: D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D023181:Antimicrobial Cationic Peptides; D000981:Antiprotozoal Agents; D053878:Hemolytic Agents; D011506:Proteins; C104501:temporin