Chip-NMR biosensor for detection and molecular analysis of cells.
Rapid and accurate measurement of biomarkers in tissue and fluid samples is a major challenge in medicine. Here we report the development of a new, miniaturized diagnostic magnetic resonance (DMR) system for multiplexed, quantitative and rapid analysis. By using magnetic particles as a proximity sensor to amplify molecular interactions, the handheld DMR system can perform measurements on unprocessed biological samples. We show the capability of the DMR system by using it to detect bacteria with high sensitivity, identify small numbers of cells and analyze them on a molecular level in real time, and measure a series of protein biomarkers in parallel. The DMR technology shows promise as a robust and portable diagnostic device.
Lee
Hakho
H
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, 185 Cambridge Street, Boston, Massachusetts 02114, USA.
Sun
Eric
E
Ham
Donhee
D
Weissleder
Ralph
R
D000818:Animals; D015415:Biomarkers / Q000737:chemistry; D015374:Biosensing Techniques; D001709:Biotechnology / Q000379:methods*; D004867:Equipment Design; D006801:Humans; D008264:Macrophages / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D051379:Mice; D046208:Microchip Analytical Procedures; D046210:Microfluidic Analytical Techniques; D053758:Nanoparticles / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D015415:Biomarkers