title: NaOCl effects on primary and permanent pulp chamber dentin.
abstract: OBJECTIVE
  The dentin quality of primary and permanent pulp chamber was inspected by Fourier-transformed Raman spectroscopy (FT-Raman) and scanning electron microscopy (SEM). Fragments of pulp chamber dentin were obtained from 20 human molar crowns (primary and permanent).
  
  
  METHODS
  The fragments were assigned to 8 groups (n=5)-Primary teeth: G1, pulp chamber dentin; G2, pulp chamber dentin irrigated with NaOCl 1% (30 min); G3, pulp chamber dentin irrigated with NaOCl 1% (30 min) and etched by 35% phosphoric acid; G4, pulp chamber dentin etched by 35% phosphoric acid. Permanent teeth: G5, pulp chamber dentin; G6, pulp chamber dentin irrigated with NaOCl 1% (30 min); G7, pulp chamber dentin irrigated with NaOCl 1% (30 min) and etched by 35% phosphoric acid; G8, pulp chamber dentin etched by 35% phosphoric acid. The spectra were subjected to the Cluster analysis. The SEM images were scored.
  
  
  RESULTS
  Inorganic content: There was a difference between primary and permanent dentin. The groups treated with NaOCl were statistically similar between them, but differed from the groups not treated. Organic content: There was no difference between primary and permanent dentin. The groups became similar after NaOCl and phosphoric acid treatments. The microscopic images showed the presence of calcospherites on permanent dentin and their absence on primary dentin.
  
  
  CONCLUSIONS
  The NaOCl changed the inorganic content in both dentitions; regardless of the following phosphoric acid etching. However, the chemical changes caused by NaOCl were not detected by SEM when it was followed by etching.
authors.affiliation: Latin American Institute of Research and Teaching in Dentistry (ILAPEO), Brazil.
authors.forename: Ana Flávia Sanches
authors.initials: AF
authors.lastname: Borges
authors.forename: Renata Andrade
authors.initials: RA
authors.lastname: Bittar
authors.forename: Fernanda Miori
authors.initials: FM
authors.lastname: Pascon
authors.forename: Lourenço Correr
authors.initials: LC
authors.lastname: Sobrinho
authors.forename: Airton Abrahão
authors.initials: AA
authors.lastname: Martin
authors.forename: Regina Maria
authors.initials: RM
authors.lastname: Puppin Rontani
mesh_terms: D000134:Acid Etching, Dental; D003786:Dental Pulp Cavity; D003804:Dentin / Q000187:drug effects*; D019229:Dentition, Permanent; D005583:Fourier Analysis; D006801:Humans; D008855:Microscopy, Electron, Scanning; D008963:Molar; D010756:Phosphoric Acids / Q000494:pharmacology; D012388:Root Canal Irrigants / Q000494:pharmacology*; D012973:Sodium Hypochlorite / Q000494:pharmacology*; D013059:Spectrum Analysis, Raman / Q000379:methods; D019228:Tooth Crown; D014094:Tooth, Deciduous
chemical_list: D010756:Phosphoric Acids; D012388:Root Canal Irrigants; D012973:Sodium Hypochlorite; C030242:phosphoric acid