title: In vitro maturation and fertilization of oocytes from unstimulated ovaries in infertile women with polycystic ovary syndrome.
abstract: OBJECTIVE
  To evaluate the effects of in vitro maturation and fertilization of oocytes from unstimulated ovaries in infertile women with polycystic ovary syndrome (PCOS).
  
  
  METHODS
  Retrospective study.
  
  
  METHODS
  Reproductive Medicine Center, First Affiliated Hospital of Wenzhou Medical College, People's Republic of China.
  
  
  METHODS
  One hundred eighteen women with PCOS undergoing 152 cycles of in vitro maturation treatment.
  
  
  METHODS
  Oocyte retrieval was carried out by ultrasound-guided puncture on days 9-14 of the cycle. The oocytes were cultured in vitro using maturation culture medium, which consisted of M-199 + 20% fetal bovine serum (FBS) + 75 mIU/mL recombinant FSH +/- 0.5 IU/mL hCG. After the oocytes had matured in vitro, fertilization and embryo transfer were performed.
  
  
  METHODS
  Rates of clinical pregnancy, multiple pregnancies, and live birth.
  
  
  RESULTS
  Relatively optimal laboratory results were obtained in this study. Embryo transfer was performed in 140 cycles, with a clinical pregnancy rate (PR) of 40.0% per transfer. Fifty-six babies have been born and there are 10 ongoing pregnancies. The overall multiple PR was 33.93%.
  
  
  CONCLUSIONS
  Our results show that using in vitro matured oocytes from unstimulated ovaries could be offered as an alternative to conventional IVF in women with PCOS, and future work should address ways to decrease the incidence of multiple pregnancies.
authors.affiliation: Reproductive Medicine Center, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang, People's Republic of China. joyce671128@163.com
authors.forename: Jun-Zhao
authors.initials: JZ
authors.lastname: Zhao
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Hong-Shan
authors.initials: HS
authors.lastname: Ge
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Huang
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Lin
mesh_terms: D000328:Adult; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005307:Fertilization in Vitro; D005640:Follicle Stimulating Hormone / Q000097:blood; D006801:Humans; D007247:Infertility, Female / Q000209:etiology / Q000503:physiopathology*; D050498:Live Birth / Q000453:epidemiology; D007986:Luteinizing Hormone / Q000097:blood; D054315:Oocyte Retrieval / Q000379:methods*; D009865:Oocytes / Q000502:physiology*; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000503:physiopathology*; D011247:Pregnancy; D012189:Retrospective Studies; D020554:Sperm Injections, Intracytoplasmic
chemical_list: D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone