[Effect of total glucosides of paeony on nuclear factor-kappaB activation in rat peritoneal macrophages].
OBJECTIVE
To study the effect of total glucosides of paeony (TGP) on lipopolysaccharides (LPS)-induced nuclear factor-kappaB (NF-kappaB) activation in macrophages.


METHODS
Rat peritoneal macrophages were pre-treated with TGP for 2 h and stimulated with LPS for 20 min or 0.5 h. Inhibitory kappaBalpha (IkappaBalpha) protein in the cytoplasm and NF-kappaB p65 protein in the nuclear were analyzed by western blot. Further, DNA binding activity of NF-kappaB complex was detected.


RESULTS
TGP enhanced the amounts of IkappaBalpha protein in the cytoplasm and decreased the amounts of NF-kappaB p65 protein in the nuclear of LPS-induced macrophages. TGP also inhibited the LPS-mediated DNA binding activity of NF-kappaB complex in macrophages.


CONCLUSIONS
TGP can inhibit LPS-induced NF-kappaB activation in macrophages through arresting IKBalpha protein degradation, NF-kappaB p65 protein nuclear translocation and DNA binding activity of NF-kappaB complex.
Chen
Gang
G
Research Center of Medical Chemistry and Chemical Biology, Chongqing Technology and Business University, Chongqing 400067, China. licoricech@ctbu.edu.cn
Deng
Xiao-Hong
XH
Guo
Li-Xia
LX
Liu
Jian-Hui
JH
D000818:Animals; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D003593:Cytoplasm / Q000187:drug effects / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005960:Glucosides / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism*; D029596:Paeonia / Q000737:chemistry*; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D051996:Transcription Factor RelA / Q000378:metabolism
D005960:Glucosides; D016328:NF-kappa B; D051996:Transcription Factor RelA; D004247:DNA