title: Tissue-specific reduction in DC-SIGN expression correlates with progression of pathogenic simian immunodeficiency virus infection.
abstract: Studies were undertaken to determine whether previously described reductions in splenic DC-SIGN expression in simian acquired immune deficiency syndrome (AIDS) are limited to pathogenic simian immunodeficiency virus (SIV) infection. DC-SIGN expression was evaluated by immunohistochemistry in lymphoid tissues from AIDS-susceptible Asian macaque monkeys as compared with AIDS-resistant sooty mangabey monkeys in the presence and absence of SIV infection. The phenotype of DC-SIGN+ cells in susceptible and resistant species was identical and most consistent with macrophage identity. Significantly lower levels of DC-SIGN expression were identified in spleen, mesenteric lymph node, and bone marrow of macaques with AIDS (P<0.05). Reduced levels of splenic DC-SIGN correlated significantly with CD4T cell depletion in long-term pathogenic infection of macaques (P<0.01), whereas SIV-infected mangabeys retained high levels of DC-SIGN expression in spleen despite persistent infection. Reduced expression of DC-SIGN in spleen specifically characterizes pathogenic forms of SIV infection, correlates with disease progression, and may contribute to SIV pathogenesis.
authors.affiliation: Division of Comparative Pathology, New England Primate Research Center, Harvard Medical School, One Pine Hill Dr. P.O. Box 9102, Southborough, MA 01772, USA.
authors.forename: Jennifer H
authors.initials: JH
authors.lastname: Yearley
authors.forename: Sarah
authors.initials: S
authors.lastname: Kanagy
authors.forename: Daniel C
authors.initials: DC
authors.lastname: Anderson
authors.forename: Karen
authors.initials: K
authors.lastname: Dalecki
authors.forename: Douglas R
authors.initials: DR
authors.lastname: Pauley
authors.forename: Carolyn
authors.initials: C
authors.lastname: Suwyn
authors.forename: Robert M
authors.initials: RM
authors.lastname: Donahoe
authors.forename: Harold M
authors.initials: HM
authors.lastname: McClure
authors.forename: Shawn P
authors.initials: SP
authors.lastname: O'Neil
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics; D016665:Cercocebus atys; D018450:Disease Progression; D015536:Down-Regulation / Q000276:immunology*; D037181:Lectins, C-Type / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics; D008221:Lymphoid Tissue / Q000276:immunology / Q000378:metabolism / Q000821:virology; D008253:Macaca mulatta; D008254:Macaca nemestrina; D008643:Mesentery; D009928:Organ Specificity / Q000276:immunology; D011956:Receptors, Cell Surface / Q000037:antagonists & inhibitors* / Q000096:biosynthesis* / Q000235:genetics; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology* / Q000378:metabolism / Q000473:pathology*; D015302:Simian Immunodeficiency Virus / Q000276:immunology* / Q000472:pathogenicity; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D015815:Cell Adhesion Molecules; D037181:Lectins, C-Type; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin