Hydrogels for potential colon drug release by thiol-ene conjugate addition of a new inulin derivative.
Inulin was chosen as a starting polymer for biocompatible, pH-sensitive and biodegradable hydrogels. Three INUDVSA-TT hydrogels were obtained by crosslinking inulin derivatives with trimethylolpropane tris(3-mercaptopropionate) under varying conditions. The resulting hydrogels were cell compatible, as demonstrated by MTS and trypan blue exclusion assays acting on Caco-2 cells, and were biodegraded by inulinase and esterase, thus suggesting their use as colonic drug delivery systems. 2-Methoxyestradiol, an anti-cancer drug, was soaked in INUDVSA-TT hydrogels and its in vitro release and apoptotic effect on Caco-2 cells were evaluated.
Pitarresi
Giovanna
G
Dipartimento di Chimica e Tecnologie Farmaceutiche, Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy. giopitar@unipa.it
Tripodo
Giuseppe
G
Calabrese
Rossella
R
Craparo
Emanuela Fabiola
EF
Licciardi
Mariano
M
Giammona
Gaetano
G
D000077584:2-Methoxyestradiol; D006690:Bisbenzimidazole; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D003106:Colon / Q000166:cytology / Q000378:metabolism*; D003432:Cross-Linking Reagents / Q000494:pharmacology; D053938:DNA Fragmentation / Q000187:drug effects; D016503:Drug Delivery Systems; D004958:Estradiol / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D020100:Hydrogels / Q000378:metabolism*; D007444:Inulin / Q000031:analogs & derivatives*; D008970:Molecular Weight; D010316:Particle Size; D013387:Succinic Anhydrides / Q000737:chemistry; D013438:Sulfhydryl Compounds / Q000737:chemistry*; D013450:Sulfones / Q000737:chemistry*
D003432:Cross-Linking Reagents; D020100:Hydrogels; D013387:Succinic Anhydrides; D013438:Sulfhydryl Compounds; D013450:Sulfones; D004958:Estradiol; C009873:divinyl sulfone; D000077584:2-Methoxyestradiol; C031801:succinic anhydride; D007444:Inulin; D006690:Bisbenzimidazole