Stimulation of peroxynitrite catalysis improves insulin sensitivity in high fat diet-fed mice.
Peroxynitrite synthesis is increased in insulin resistant animals and humans. Peroxynitirite-induced nitration of insulin signalling proteins impairs insulin action in vitro, but the role of peroxynitrite in the pathogenesis of insulin resistance in vivo is not known. We therefore assessed the effects of a 1-week treatment with the peroxynitrite decomposition catalyst FeTPPS on insulin sensitivity in insulin resistant high fat diet-fed (HFD) and control mice. FeTPPS normalized the fasting plasma glucose and insulin levels (P < 0.01), attenuated the hyperglycaemic response to an intraperitoneal glucose challenge by roughly 50% (P < 0.05), and more than doubled the insulin-induced decrease in plasma glucose levels in HFD-fed mice (P < 0.001). Moreover, FeTPPS restored insulin-stimulated Akt phosphorylation and insulin-stimulated glucose uptake in isolated skeletal muscle in vitro. Stimulation of peroxynitrite catalysis attenuates HFD-induced insulin resistance in mice by restoring insulin signalling and insulin-stimulated glucose uptake in skeletal muscle tissue.
Duplain
Hervé
H
Department of Internal Medicine and Botnar Center for Clinical Research, University Hospital Center and University of Lausanne, Switzerland. herve.duplain@chuv.ch
Sartori
Claudio
C
Dessen
Pierre
P
Jayet
Pierre-Yves
PY
Schwab
Marcos
M
Bloch
Jonathan
J
Nicod
Pascal
P
Scherrer
Urs
U
D000818:Animals; D001786:Blood Glucose / Q000032:analysis*; D002384:Catalysis / Q000187:drug effects; D004041:Dietary Fats / Q000378:metabolism*; D007333:Insulin Resistance / Q000502:physiology*; D008297:Male; D008665:Metalloporphyrins / Q000008:administration & dosage*; D051379:Mice; D008810:Mice, Inbred C57BL; D030421:Peroxynitrous Acid / Q000378:metabolism*
C466420:5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron(III) chloride; D001786:Blood Glucose; D004041:Dietary Fats; D008665:Metalloporphyrins; D030421:Peroxynitrous Acid