Prolonged stochastic single ion channel recordings in S-layer protein stabilized lipid bilayer membranes.
S-layer proteins are commonly found in bacteria and archaea as two-dimensional monomolecular crystalline arrays as the outermost cell membrane component. These proteins have the unique property that following disruption by chemical agents, monomers of the protein can re-assemble to their original lattice structure. This unique property makes S-layers interesting for utilization in bio-nanotechnological applications. Here, we show that the addition of S-layer proteins to bilayer lipid membranes increases the lifetime and the stability of the bilayer. M2delta ion channels were functionally incorporated into these S-layer stabilized membranes and we were able to record their activity for up to 20 h. Transmission electron microscopy (TEM) was used to visualize the 2D crystalline pattern of the S-layer and the M2delta ion channel characteristics in bilayer lipid membrane's were compared in the presence and absence of S-layers.
Keizer
Henk M
HM
Department of Chemistry, Physical Chemistry, University of Florida, 318 Leigh Hall, FL 32611, Gainesville, USA.
Andersson
Martin
M
Chase
Chris
C
Laratta
William P
WP
Proemsey
Josh B
JB
Tabb
Joel
J
Long
Joanna R
JR
Duran
Randolph S
RS
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D007473:Ion Channels / Q000502:physiology*; D008051:Lipid Bilayers; D008562:Membrane Glycoproteins / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D013269:Stochastic Processes
D001426:Bacterial Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D008562:Membrane Glycoproteins; C055764:S-layer proteins