title: Treatment with bone marrow-derived stromal cells accelerates wound healing in diabetic rats.
abstract: Bone marrow stem cells participate in tissue repair processes and may have a role in wound healing. Diabetes is characterised by delayed and poor wound healing. We investigated the potential of bone marrow-derived mesenchymal stromal cells (BMSCs) to promote healing of fascial wounds in diabetic rats. After manifestation of streptozotocin (STZ)-induced diabetic state for 5 weeks in male adult Sprague-Dawley rats, healing of fascial wounds was severely compromised. Compromised wound healing in diabetic rats was characterised by excessive polymorphonuclear cell infiltration, lack of granulation tissue formation, deficit of collagen and growth factor [transforming growth factor (TGF-beta), epidermal growth factor (EGF), vascular endothelial growth factor (VEGF), platelet-derived growth factor PDGF-BB and keratinocyte growth factor (KGF)] expression in the wound tissue and significant decrease in biomechanical strength of wounds. Treatment with BMSC systemically or locally at the wound site improved the wound-breaking strength (WBS) of fascial wounds. The improvement in WBS was associated with an immediate and significant increase in collagen levels (types I-V) in the wound bed. In addition, treatment with BMSCs increased the expression of growth factors critical to proper repair and regeneration of the damaged tissue moderately (TGF-beta, KGF) to markedly (EGF, VEGF, PDGF-BB). These data suggest that cell therapy with BMSCs has the potential to augment healing of the diabetic wounds.
authors.affiliation: Department of General Surgery, Henry Ford Health System, Detroit, MI, USA.
authors.forename: David S
authors.initials: DS
authors.lastname: Kwon
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Gao
authors.forename: Yong Bo
authors.initials: YB
authors.lastname: Liu
authors.forename: Deborah S
authors.initials: DS
authors.lastname: Dulchavsky
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Danyluk
authors.forename: Mona
authors.initials: M
authors.lastname: Bansal
authors.forename: Michael
authors.initials: M
authors.lastname: Chopp
authors.forename: Kevin
authors.initials: K
authors.lastname: McIntosh
authors.forename: Ali S
authors.initials: AS
authors.lastname: Arbab
authors.forename: Scott A
authors.initials: SA
authors.lastname: Dulchavsky
authors.forename: Subhash C
authors.initials: SC
authors.lastname: Gautam
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001696:Biomechanical Phenomena; D001854:Bone Marrow Cells; D016026:Bone Marrow Transplantation / Q000379:methods*; D003094:Collagen / Q000032:analysis / Q000502:physiology; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000150:complications*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D006097:Granulation Tissue / Q000502:physiology; D036341:Intercellular Signaling Peptides and Proteins / Q000032:analysis / Q000502:physiology; D008297:Male; D008648:Mesoderm / Q000166:cytology; D051381:Rats; D017207:Rats, Sprague-Dawley; D013311:Streptozocin; D017154:Stromal Cells / Q000637:transplantation*; D013718:Tensile Strength; D015854:Up-Regulation; D014945:Wound Healing / Q000502:physiology*; D014950:Wounds, Penetrating / Q000209:etiology / Q000473:pathology / Q000628:therapy*
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D013311:Streptozocin; D003094:Collagen