Characterization of a mouse model of plague after aerosolization of Yersinia pestis CO92.
Yersinia pestis is a Gram-negative bacterium, and the causative agent of bubonic plague and pneumonic plague. Because of its potential use as a biological warfare weapon, the plague bacterium has been placed on the list of category A select agents. The dynamics of pneumonic infection following aerosolization of the highly virulent Y. pestis CO92 strain have been poorly studied; therefore, the purpose of this study was to determine the LD(50) dose, bacterial dissemination, cytokine/chemokine production and tissue damage in Swiss-Webster mice over a 72 h course of infection. We exposed mice in a whole-body Madison chamber to various doses of Y. pestis CO92 aerosolized by a Collison nebulizer, and determined that the LD(50) presented dose (Dp) of the bacterium in the lungs was 2.1 x 10(3) c.f.u. In a subsequent study, we infected mice at a Dp of 1.3 x 10(4) c.f.u., and harvested organs and blood at 1, 24, 48 and 72 h post-infection. Histopathological examination, in addition to measurement of bacterial dissemination and cytokine/chemokine analysis, indicated progressive tissue injury, and an increased number of animals succumbing to infection over the course of the experiment. Using these data, we were able to characterize the mouse plague model following aerosolization of Y. pestis CO92.
Agar
Stacy L
SL
Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Sha
Jian
J
Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Foltz
Sheri M
SM
Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Erova
Tatiana E
TE
Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Walberg
Kristin G
KG
Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Parham
Todd E
TE
Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Baze
Wallace B
WB
University of Texas M. D. Anderson Cancer Center, Bastrop, TX 78602, USA.
Suarez
Giovanni
G
Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Peterson
Johnny W
JW
Center for Biodefense and Emerging Infections and Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555, USA.|Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
Chopra
Ashok K
AK
Center for Biodefense and Emerging Infections and Sealy Center for Vaccine Development, University of Texas Medical Branch, Galveston, TX 77555, USA.|Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, USA.
D000336:Aerosols / Q000032:analysis; D000818:Animals; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D007928:Lethal Dose 50; D008168:Lung / Q000276:immunology / Q000382:microbiology / Q000473:pathology; D051379:Mice; D010930:Plague / Q000276:immunology / Q000382:microbiology* / Q000473:pathology / Q000635:transmission*; D015010:Yersinia pestis / Q000254:growth & development* / Q000276:immunology / Q000472:pathogenicity
D000336:Aerosols; D016207:Cytokines