title: [Several carotenoids of vegetable determinated by high performance liquid chromatography].
abstract: OBJECTIVE
  To detect alpha-carotene (alpha-C), beta-carotene (beta-C), cryptoxanthin (Cryp), lutein (Lut) and lycopene (Lyco) levels in vegetables.
  
  
  METHODS
  A HPLC method with C30 column was used to detect several carotenoids at the wave length of 450 nm. A gradient elution method with methanol, methyl-t-butyl-ether and ammonium acetate solution was used to separate the carotenoids.
  
  
  RESULTS
  The level of Lut, Cryp, alpha-c, beta-c and lycopene were linearly related with the peak area. The recoveries of the standard were between 94.7%-108.1%. The relative standard deviations were less than 4.62%.
  
  
  CONCLUSIONS
  The method could be simple and credible. It could separate 5 carotenoids in vegetables simultaneously.
authors.affiliation: Institute for Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Xianfeng
authors.initials: X
authors.lastname: Zhao
authors.forename: Lili
authors.initials: L
authors.lastname: Pan
authors.forename: Jing
authors.initials: J
authors.lastname: Meng
authors.forename: Yin
authors.initials: Y
authors.lastname: Wang
authors.lastname: et al
mesh_terms: D002338:Carotenoids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D065366:Cryptoxanthins; D014675:Vegetables / Q000737:chemistry*; D024341:Xanthophylls / Q000032:analysis; D019207:beta Carotene / Q000032:analysis
chemical_list: D065366:Cryptoxanthins; D024341:Xanthophylls; D019207:beta Carotene; D002338:Carotenoids; C041635:alpha-carotene