Adenosine kinase gene expression in human colorectal cancer.
Real-time reverse transcription polymerase chain reaction (qRT-PCR) was used to evaluate gene expression of adenosine kinase, a key enzyme in adenosine metabolism, in human intestinal biopsy specimens of 10 colorectal cancer patients. Quantitative mRNA expression levels were normalized against the reference gene beta-actin. The results showed that adenosine kinase gene expression was significantly higher in cancer than in normal-appearing tissue, in line with our previous measurements of adenosine kinase enzyme activities in colorectal tumor samples.
Giglioni
S
S
Dipartimento di Medicina Interna, Sc. Endocrino-Metaboliche e Biochimica, Università di Siena, Italy.
Leoncini
R
R
Aceto
E
E
Chessa
A
A
Civitelli
S
S
Bernini
A
A
Tanzini
G
G
Carraro
F
F
Pucci
A
A
Vannoni
D
D
D000241:Adenosine / Q000378:metabolism; D000248:Adenosine Kinase / Q000235:genetics* / Q000378:metabolism; D000368:Aged; D000369:Aged, 80 and over; D001706:Biopsy; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000201:enzymology* / Q000235:genetics* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007422:Intestines / Q000201:enzymology / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000201:enzymology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
D012333:RNA, Messenger; D000248:Adenosine Kinase; D000241:Adenosine