Quantum dot nanocrystals having guanosine imprinted nanoshell for DNA recognition.
Molecular imprinted polymers (MIPs) as a recognition element for sensors are increasingly of interest and MIP nanoparticles have started to appear in the literature. In this study, we have proposed a novel thiol ligand-capping method with polymerizable methacryloylamido-cysteine (MAC) attached to CdS quantum dots (QDs), reminiscent of a self-assembled monolayer and have reconstructed surface shell by synthetic host polymers based on molecular imprinting method for DNA recognition. In this method, methacryloylamidohistidine-platinium (MAH-Pt(II)) is used as a new metal-chelating monomer via metal coordination-chelation interactions and guanosine templates of DNA. Nanoshell sensors with guanosine templates give a cavity that is selective for guanosine and its analogues. The guanosine can simultaneously chelate to Pt(II) metal ion and fit into the shape-selective cavity. Thus, the interaction between Pt(II) ion and free coordination spheres has an effect on the binding ability of the CdS QD nanosensor. The binding affinity of the guanosine imprinted nanocrystals has investigated by using the Langmuir and Scatchard methods, and experiments have shown the shape-selective cavity formation with O6 and N7 of a guanosine nucleotide (K(a) = 4.841x10(6) mol L(-1)) and a free guanine base (K(a) = 0.894x10(6) mol L(-1)). Additionally, the guanosine template of the nanocrystals is more favored for single stranded DNA compared to double stranded DNA.
Diltemiz
Sibel Emir
SE
Department of Chemistry, Anadolu University, Eskişehir, Turkey.
Say
Ridvan
R
Büyüktiryaki
Sibel
S
Hür
Deniz
D
Denizli
Adil
A
Ersöz
Arzu
A
D019187:Cadmium Compounds / Q000737:chemistry* / Q000378:metabolism; D002614:Chelating Agents / Q000737:chemistry / Q000378:metabolism; D003545:Cysteine / Q000031:analogs & derivatives / Q000737:chemistry; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D006151:Guanosine / Q000737:chemistry*; D008024:Ligands; D049449:Luminescence; D009682:Magnetic Resonance Spectroscopy; D046529:Microscopy, Electron, Transmission; D054802:Molecular Imprinting / Q000379:methods*; D010984:Platinum / Q000737:chemistry; D045663:Quantum Dots; D013379:Substrate Specificity; D013438:Sulfhydryl Compounds / Q000737:chemistry; D013440:Sulfides / Q000737:chemistry* / Q000378:metabolism
D019187:Cadmium Compounds; D002614:Chelating Agents; D008024:Ligands; D013438:Sulfhydryl Compounds; D013440:Sulfides; C034939:cadmium sulfide; D006151:Guanosine; D010984:Platinum; D004247:DNA; D003545:Cysteine