Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study.
We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.
Brazhe
Alexey R
AR
Technical University of Denmark, Department of Physics, 2800 Kongens Lyngby, Denmark. brazhe@fysik.dtu.dk
Brazhe
Nadezda A
NA
Maksimov
Georgy V
GV
Ignatyev
Pavel S
PS
Rubin
Andrey B
AB
Mosekilde
Erik
E
Sosnovtseva
Olga V
OV
D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007368:Interferometry / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000379:methods*