Expression of bone specific alkaline phosphatase on human B cells.
We here report for the first time that human B cell express bone specific alkaline phosphatase. Using a monoclonal antibody against bone specific alkaline phosphatase (BAP), its expression was detected in approximately 50% of circulating B cells but not T cells. Using RT-PCR, BAP specific transcripts were detected in the B cells expressing surface BAP but not in those not expressing BAP. Activation of B lymphocytes using phorbal ester, PMA with or without ionomycin did not affect change in BAP expression. Following stimulations with Pokeweed Mitogen (PWM) and Staphylococcus aureus Cowan I (SAC), BAP expression on human B cells was increased. This paralleled the increase in production of immunoglobulins and BAP, detected by ELISA and enzyme assay with p-nitrophenyl phosphate, respectively. The role of BAP in B cell biology is uncertain. However, these data suggest that BAP may play an important role in differentiation and maturation of human B cells.
Hossain
Ashfaque
A
Division of Pediatric Rheumatology/Immunology, Department of Pediatrics, Creighton University School of Medicine, 2500 California Plaza, Omaha, NE 68178, USA.
Jung
Lawrence K
LK
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001402:B-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D001842:Bone and Bones / Q000201:enzymology*; D002478:Cells, Cultured; D006801:Humans; D007136:Immunoglobulins / Q000378:metabolism; D015759:Ionomycin / Q000494:pharmacology; D007476:Ionophores / Q000494:pharmacology; D017493:Leukocyte Common Antigens / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D008213:Lymphocyte Activation; D016131:Lymphocyte Subsets / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D051379:Mice; D011043:Pokeweed Mitogens / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000378:metabolism
D000911:Antibodies, Monoclonal; D007136:Immunoglobulins; D007476:Ionophores; D011043:Pokeweed Mitogens; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; D015759:Ionomycin; D000469:Alkaline Phosphatase; D017493:Leukocyte Common Antigens; D013755:Tetradecanoylphorbol Acetate