title: [Preparation and characterization of specific monoclonal antibodies against hexon of HAdV 3].
abstract: OBJECTIVE
  To obtain the monoclonal antibody against hexon protein of human adenovirus.
  
  
  METHODS
  BALB/c mice were immunized with purified recombinant hexon protein, and the spleen cells of the mice were isolated and fused with myloma cells. Four hybridoma cell strains were screened by indirect ELISA and cultured, and the sensitivity, specificity and virus neutralizing activity were analyzed with ELISA, Western blotting and neutralizing test.
  
  
  RESULTS
  The mouse ascites produced by these hybridoma cells contained specific monoclonal antibodies against hexon protein of human adenovirus as identified by ELISA and Western blot, and the antibody generated by 4C6 strain showed human adenovirus type 3-neutralizing activity.
  
  
  CONCLUSIONS
  The monoclonal antibodies against hexon protein with high specificity have been successfully obtained, and these antibodies can be useful in developing assays for early diagnosis of HAdV3 infection and also in study of therapeutic drugs of the infection.
authors.affiliation: South China Institute of Oceanology, Guangzhou 510301, China. zhou3218@yahoo.com
authors.forename: Rong
authors.initials: R
authors.lastname: Zhou
authors.forename: Hui-Ying
authors.initials: HY
authors.lastname: Sheng
authors.forename: Xin-Gui
authors.initials: XG
authors.lastname: Tian
authors.forename: Chang-Bing
authors.initials: CB
authors.lastname: Wang
authors.forename: Si-Tang
authors.initials: ST
authors.lastname: Gong
authors.forename: Qiao-Lian
authors.initials: QL
authors.lastname: Chen
mesh_terms: D000260:Adenoviruses, Human / Q000737:chemistry / Q000276:immunology*; D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology; D000914:Antibodies, Viral / Q000096:biosynthesis* / Q000276:immunology; D015153:Blotting, Western; D036022:Capsid Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D004926:Escherichia coli / Q000235:genetics; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D036022:Capsid Proteins; D011994:Recombinant Proteins; C018315:hexon capsid protein, Adenovirus