Structure of Ynk1 from the yeast Saccharomyces cerevisiae.
Nucleoside diphosphate kinase (NDPK) catalyzes the transfer of the gamma-phosphate from nucleoside triphosphates to nucleoside diphosphates. In addition to biochemical studies, a number of crystal structures of NDPK from various organisms, including both native proteins and complexes with nucleotides or nucleotide analogues, have been determined. Here, the crystal structure of Ynk1, an NDPK from the yeast Saccharomyces cerevisiae, has been solved at 3.1 A resolution. Structural analysis strongly supports the oligomerization state of this protein being hexameric rather than tetrameric.
Wang
Huabing
H
Protein Research Institute, Tongji University, Shanghai 200092, People's Republic of China.
Bao
Rui
R
Jiang
Chunhui
C
Yang
Zhu
Z
Zhou
Cong Zhao
CZ
Chen
Yuxing
Y
D000595:Amino Acid Sequence / Q000235:genetics; D017124:Conserved Sequence; D018360:Crystallography, X-Ray; D019281:Dimerization; D024101:Mitochondrial Proteins / Q000737:chemistry*; D008969:Molecular Sequence Data; D009701:Nucleoside-Diphosphate Kinase / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D012441:Saccharomyces cerevisiae / Q000201:enzymology*; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*
D024101:Mitochondrial Proteins; D029701:Saccharomyces cerevisiae Proteins; D009701:Nucleoside-Diphosphate Kinase; C473137:YNK1 protein, S cerevisiae