title: Analysis of lolB gene sequence and its use in the development of a PCR assay for the detection of Vibrio cholerae.
abstract: A PCR assay has been developed based on a lolB (hemM) gene, which was found to be highly conserved among the Vibrio cholerae species but non-conserved among the other enteric bacteria. The lolB PCR detected all O1, O139 and non-O1/non-O139 serogroup and biotypes of V. cholerae. The analytical specificity of this assay was 100% while the analytical sensitivity was 10 pg/microL and 10(3) CFU/mL at DNA and bacterial level respectively. The diagnostic sensitivity and specificity was 98.5% and 100% respectively.
authors.affiliation: School of Health Science, Universiti Sains Malaysia, Malaysia. lalitha@kb.usm.my
authors.forename: P
authors.initials: P
authors.lastname: Lalitha
authors.forename: M N
authors.initials: MN
authors.lastname: Siti Suraiya
authors.forename: K L
authors.initials: KL
authors.lastname: Lim
authors.forename: S Y
authors.initials: SY
authors.lastname: Lee
authors.forename: A R
authors.initials: AR
authors.lastname: Nur Haslindawaty
authors.forename: Y Y
authors.initials: YY
authors.lastname: Chan
authors.forename: Asma
authors.initials: A
authors.lastname: Ismail
authors.forename: Zainul F
authors.initials: ZF
authors.lastname: Zainuddin
authors.forename: M
authors.initials: M
authors.lastname: Ravichandran
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D003304:Coproporphyrinogen Oxidase / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014735:Vibrio Infections / Q000382:microbiology*; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification*
chemical_list: D001426:Bacterial Proteins; D003304:Coproporphyrinogen Oxidase; D004269:DNA, Bacterial; C109194:HemN protein, Bacteria