title: CD27 and CD70 do not play a critical role in the development of experimental allergic conjunctivitis in mice.
abstract: CD27, which belongs to the TNF receptor family, is a costimulatory molecule that participates in T-cell activation. Unlike costimulatory molecules such as OX40 and 4-1BB, little is known about the role CD27 plays a role in the development of experimental diseases. We asked whether CD27 and its ligand CD70 participate in the development of experimental allergic conjunctivitis (EC) in BALB/c mice, which is generated by immunization with ragweed (RW) in alum and challenged 10 days later with RW in eye drops. The roles of CD27 and CD70 were tested by intraperitoneally injecting the mice with anti-CD27, anti-CD70 or a control Ab during the induction or effector phase. Twenty-four hours after challenge, the conjunctivas, blood and spleens were harvested for histological analysis, measuring Ig levels and cytokine analysis, respectively. Regardless of when the mice were treated, anti-CD27 or anti-CD70 Ab treatment did not significantly affect the severity of EC as evaluated by conjunctival eosinophil numbers. However, anti-CD27 or anti-CD70 Ab treatment during the induction phase did significantly modulate systemic humoral and cellular immune responses. In vitro treatment of RW-primed splenocytes with anti-CD27 or anti-CD70 Ab did not affect the EC-inducing capability of the splenocytes. Taken together, CD27 and CD70 do not play a critical role in the development of EC.
authors.affiliation: Department of Ophthalmology and Visual Science, Kochi Medical School, Juntendo University School of Medicine, Kohasu, Oko-cho, Nankoku-city 783-8505, Japan.
authors.forename: Tamaki
authors.initials: T
authors.lastname: Sumi
authors.forename: Waka
authors.initials: W
authors.lastname: Ishida
authors.forename: Ayako
authors.initials: A
authors.lastname: Ojima
authors.forename: Minako
authors.initials: M
authors.lastname: Kajisako
authors.forename: Tamami
authors.initials: T
authors.lastname: Sakanishi
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Atsuki
authors.initials: A
authors.lastname: Fukushima
mesh_terms: D019264:Adoptive Transfer; D000485:Allergens / Q000276:immunology*; D000534:Alum Compounds / Q000008:administration & dosage; D031164:Ambrosia / Q000276:immunology*; D000818:Animals; D019138:Antibodies, Blocking / Q000008:administration & dosage; D053282:CD27 Ligand / Q000276:immunology* / Q000378:metabolism; D002457:Cell Extracts; D003233:Conjunctivitis, Allergic / Q000209:etiology / Q000276:immunology* / Q000503:physiopathology; D016207:Cytokines / Q000378:metabolism; D004804:Eosinophils / Q000166:cytology / Q000187:drug effects / Q000276:immunology*; D008213:Lymphocyte Activation / Q000187:drug effects; D051379:Mice; D008807:Mice, Inbred BALB C; D011058:Pollen / Q000276:immunology*; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology; D012720:Severity of Illness Index; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7 / Q000276:immunology* / Q000378:metabolism; D014611:Vaccination
chemical_list: D000485:Allergens; D000534:Alum Compounds; D019138:Antibodies, Blocking; D053282:CD27 Ligand; D002457:Cell Extracts; D016207:Cytokines; D018127:Tumor Necrosis Factor Receptor Superfamily, Member 7; C041524:aluminum sulfate