Effect of Allium sativum (garlic) diallyl disulfide (DADS) on human non-small cell lung carcinoma H1299 cells.
This study was undertaken to elucidate the effect of diallyl disulfide from Allium sativum, an oil-soluble organosulfur compound found in garlic, in suppressing human non-small cell lung carcinoma H1299 cells. A potent increase in apoptotic cells has accompanied 1) a decrease in cell viability, 2) an increase of the fraction of G2/M-phase cells by up to 48.80 %, and 3) a transient increase of the phospho-p42/44 (phosphorylated p42/44 MAPK) in a time- and concentration-dependent manner. These results indicated that diallyl disulfide could induce apoptosis in human non-small cell lung carcinoma H1299 cells via, at least partly, G2/M-phase block of the cell cycle, related to a rise in MAPK phosphorylation.
Hui
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School of Chemistry and Chemical Engineering, Nantong University, Nantong, Jiangsu 226007, P. R. China.
Jun
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Ya
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Ming
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D000498:Allyl Compounds / Q000494:pharmacology*; D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002289:Carcinoma, Non-Small-Cell Lung; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D004220:Disulfides / Q000494:pharmacology*; D004789:Enzyme Activation; D016195:G2 Phase / Q000187:drug effects; D005737:Garlic / Q000737:chemistry*; D006801:Humans; D008168:Lung / Q000166:cytology / Q000187:drug effects; D008175:Lung Neoplasms; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects
D000498:Allyl Compounds; D016588:Anticarcinogenic Agents; D004220:Disulfides; C028009:diallyl disulfide; D019950:Mitogen-Activated Protein Kinase 1; D048052:Mitogen-Activated Protein Kinase 3