title: Proteomic analysis of cardiac metabolic enzymes in asphyxiated newborn piglets.
abstract: Hypoxia/reoxygenation (H/R) creates an energetic deficiency in the heart, which may contribute to myocardial dysfunction. We hypothesized that H/R-induced impairment of cardioenergetic enzymes occurs in asphyxiated newborn animals. After hypoxia for 2 h (10-15% oxygen), newborn piglets were resuscitated with 100% oxygen for 1 h, followed by 21% oxygen for 3 h. Sham-operated control piglets had no H/R. Hemodynamic parameters in the piglets were continuously measured. At the end of experiment, hearts were isolated for proteomic analysis. In asphyxiated hearts, the level of isocitrate dehydrogenase and malate dehydrogenase was reduced compared to controls. Inverse correlations between the level of myocardial malate dehydrogenase and cardiac function were observed in the control, but not the H/R hearts. We conclude that reoxygenation of asphyxiated newborn piglets reduces the level of myocardial isocitrate dehydrogenase and malate dehydrogenase. While the cause is not clear, it may be related to the impaired tricarboxylic acid cycle pathway and energy production in the heart.
authors.affiliation: Deparment of Pharmacology, University of Saskatchewan, Saskatoon, SK, Canada.
authors.forename: Justyna
authors.initials: J
authors.lastname: Fert-Bober
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Sawicki
authors.forename: Gary D
authors.initials: GD
authors.lastname: Lopaschuk
authors.forename: Po-Yin
authors.initials: PY
authors.lastname: Cheung
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001237:Asphyxia / Q000201:enzymology* / Q000503:physiopathology; D015180:Electrophoresis, Gel, Two-Dimensional; D006334:Heart Function Tests; D006439:Hemodynamics; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood; D008291:Malate Dehydrogenase / Q000378:metabolism; D009206:Myocardium / Q000201:enzymology*; D010313:Partial Pressure; D040901:Proteomics; D034421:Sus scrofa
chemical_list: D019344:Lactic Acid; D008291:Malate Dehydrogenase; D007521:Isocitrate Dehydrogenase