Hydrogen peroxide biosensor with a supramolecular layer-by-layer design.
A new sensor design is reported for the construction of an amperometric enzyme biosensor toward H (2)O(2). It was based in the supramolecular immobilization of alternating layers of horseradish peroxidase (either modified with 1-adamantane or beta-cyclodextrin-branched carboxymethylcellulose residues) on Au electrodes coated with polythiolated beta-cyclodextrin polymer. The analytical response of the electrodes, using 1 mM hydroquinone as an electrochemical mediator, increases when the number of enzyme layers increases. The biosensor having three enzyme layers showed a sensitivity of 720 microA/M cm (2) and a detection limit of 2 microM and retained 96% of its initial activity after 30 days of storage. The host-guest supramolecular nature of the immobilization method was confirmed by cyclic voltammetry.
Camacho
Conrado
C
Center for Enzyme Technology, University of Matanzas, Matanzas 44740, Cuba.
Matías
Juan C
JC
Cao
Roberto
R
Matos
Madyu
M
Chico
Belkis
B
Hernández
Javier
J
Longo
Maria A
MA
Sanromán
Maria A
MA
Villalonga
Reynaldo
R
D015374:Biosensing Techniques; D003505:Cyclodextrins / Q000737:chemistry; D006735:Horseradish Peroxidase / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry*
D003505:Cyclodextrins; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase