title: Quantitative analysis of rabeprazole sodium in commercial dosage forms by spectrophotometry.
abstract: The main aim of this work is to develop and validate two spectrophotometric methods for the quantitative analysis of rabeprazole sodium in commercial dosage forms. Method A is based on the reaction of drug with 3-methyl-2-benzothiazolinone hydrazone hydrochloride (MBTH) in the presence of ammonium cerium(IV) nitrate in acetic acid medium at room temperature to form red-brown product which absorbs maximally at 470 nm. Method B utilizes the reaction of rabeprazole sodium with 1-chloro-2,4-dinitrobenzene (CDNB) in dimethyl sulfoxide (DMSO) at 45+/-1 degrees C to form yellow colored Meisenheimer complex. The colored complex has a characteristic band peaking at 420 nm. Under the optimized reaction conditions, proposed methods are validated as per ICH guidelines. Beer's law is obeyed in the concentration ranges of 14-140 and 7.5-165 microg ml(-1) with linear regression equations of A=6.041 x 10(-4)+1.07 x 10(-2)C and A=1.020 x 10(-3)+5.0 x 10(-3)C for methods A and B, respectively. The limits of detection for methods A and B are 1.38 and 0.75 microg ml(-1), respectively. Both methods have been applied successfully for the estimation of rabeprazole sodium in commercial dosage forms. The results are compared with the reference UV spectrophotometric method.
authors.affiliation: Department of Chemistry, Aligarh Muslim University, Aligarh, Uttar Pradesh, India. cht17nr@yahoo.co.in
authors.forename: Nafisur
authors.initials: N
authors.lastname: Rahman
authors.forename: Zehra
authors.initials: Z
authors.lastname: Bano
authors.forename: Syed Najmul Hejaz
authors.initials: SN
authors.lastname: Azmi
mesh_terms: D053799:2-Pyridinylmethylsulfinylbenzimidazoles / Q000032:analysis*; D064750:Rabeprazole; D013053:Spectrophotometry / Q000379:methods*; D013607:Tablets; D013696:Temperature; D013997:Time Factors
chemical_list: D053799:2-Pyridinylmethylsulfinylbenzimidazoles; D013607:Tablets; D064750:Rabeprazole