title: Plasmodium yoelii: novel rhoptry proteins identified within the body of merozoite rhoptries in rodent Plasmodium malaria.
abstract: The biogenesis, organization and function of the rhoptries are not well understood. Antisera were prepared to synthetic peptides prepared as multiple antigenic peptides (MAPs) obtained from a Plasmodium yoelii merozoite rhoptry proteome analysis. The antisera were used in immunofluorescence and immunoelectron microscopy of schizont-infected erythrocytes. Twenty-seven novel rhoptry proteins representing proteases, metabolic enzymes, secreted proteins and hypothetical proteins, were identified in the body of the rhoptries by immunoelectron microscopy. The merozoite rhoptries contain a heterogeneous mixture of proteins that may initiate host cell invasion and establish intracellular parasite development.
authors.affiliation: Department of Biological, Geological and Environmental Sciences, Cleveland State University, 2121 Euclid Avenue, SI 219, Cleveland OH 44115, USA. t.sam-yellowe@csuohio.edu
authors.forename: Tobili Y
authors.initials: TY
authors.lastname: Sam-Yellowe
authors.forename: Tashara L
authors.initials: TL
authors.lastname: Banks
authors.forename: Hisashi
authors.initials: H
authors.lastname: Fujioka
authors.forename: Judith A
authors.initials: JA
authors.lastname: Drazba
authors.forename: Satya P
authors.initials: SP
authors.lastname: Yadav
mesh_terms: D000818:Animals; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D008288:Malaria / Q000469:parasitology*; D008297:Male; D052940:Merozoites / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D016253:Microscopy, Immunoelectron; D015388:Organelles / Q000737:chemistry / Q000648:ultrastructure; D010967:Plasmodium yoelii / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D011817:Rabbits
chemical_list: D015800:Protozoan Proteins