Home-made capillary array electrophoresis for high-throughput amino acid analysis.
A capillary array electrophoresis (CAE) with confocal rotary scanner for high-throughput carboxytetramethylrhodamine succinimidyl ester (TAMRA)-labeled amino acid (AA) analysis is presented. Performance of the CAE setup was evaluated with AA samples. Up to 128 capillaries could be detected in parallel. For the first time, the device was applied to separate the enantiomers of isoleucine, cysteine, and glutamic acid with cyclodextrin-modified electrolytes by capillary zone electrophoresis. Baseline separation of seven AAs is also demonstrated using micellar electrokinetic chromatography method.
Liu
Kaiying
K
State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Dalian, 116023, China.
Wang
Hui
H
Bai
Jiling
J
Wang
Li
L
D000596:Amino Acids / Q000032:analysis* / Q000737:chemistry; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D012680:Sensitivity and Specificity; D013237:Stereoisomerism
D000596:Amino Acids