title: [Inhibitory effects of sinapine on activity of acetylcholinesterase in cerebral homogenate and blood serum of rats].
abstract: OBJECTIVE
  The present study investigated the inhibitory effects of Chinese herb component sinapine on activity of acetylcholinesterase (AChE) in cerebral homogenate and blood serum of rats.
  
  
  METHODS
  AChE was prepared from cerebral homogenate and blood serum of rats, respectively. Acetylcholinesterase activity assay kit and Chromatometry were used to detect the AChE activity.
  
  
  RESULTS
  Sinapine significantly inhibited AChE activity in vitro, with more effective on cerebral homogenate (IC50 3.66 micromol x L(-1)) than blood serum (IC50 22.1 micromol x L(-1)).
  
  
  CONCLUSIONS
  Sinapine could significantly inhibit the cerebral AChE activity and may be a promising drug used for prevention and cure of Alzheimer's disease as a cholinesterase inhibitor.
authors.affiliation: Postdoctoral scientific research afflux station, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
authors.forename: Ling
authors.initials: L
authors.lastname: He
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Li
authors.forename: Sheng-Wei
authors.initials: SW
authors.lastname: Guo
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Liu
authors.forename: Jia-Bin
authors.initials: JB
authors.lastname: Qiu
authors.forename: Fu
authors.initials: F
authors.lastname: Li
authors.forename: Bao-Chang
authors.initials: BC
authors.lastname: Cai
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood* / Q000378:metabolism*; D000544:Alzheimer Disease / Q000188:drug therapy / Q000517:prevention & control; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002794:Choline / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D002800:Cholinesterase Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats
chemical_list: D002800:Cholinesterase Inhibitors; C005685:sinapine; D000110:Acetylcholinesterase; D002794:Choline