Regulation of AMPA receptor trafficking by delta-catenin.
delta-catenin is a protein that binds to the classical cadherins and to synaptic scaffolding proteins in a manner which allows the protein to serve as a link between the adherens junction and the postsynaptic complex. Here we show the regulatory role of delta-catenin on synaptic transmission. delta-catenin increased the AMPA receptor-mediated EPSC, but had no significant effect on the NMDA receptor-mediated EPSC. The effect of delta-catenin on the AMPAR EPSC was mediated by its PDZ ligand. delta-catenin regulates the surface expression of GluR2 in the dendritic spines of neurons. Immunoprecipitation revealed that delta-catenin bound to GRIP-1. In COS7 cells, co-transfection of delta-catenin, GRIP and GluR2 showed that delta-catenin regulates the membrane localization of GRIP through its PDZ ligand and increased the surface expression of GluR2. This study directly shows that delta-catenin is essential for the trafficking and positioning GluR2 in the spine and thus further links delta-catenin to neuronal plasticity.
Ochiishi
Tomoyo
T
Neuroscience Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan. tomoyo.ochiishi@aist.go.jp
Futai
Kensuke
K
Okamoto
Kenichi
K
Kameyama
Kimihiko
K
Kosik
Kenneth S
KS
D000818:Animals; D019556:COS Cells; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D051177:Catenins; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D002522:Chlorocebus aethiops; D019706:Excitatory Postsynaptic Potentials / Q000502:physiology; D006624:Hippocampus / Q000166:cytology; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism; D009473:Neuronal Plasticity / Q000502:physiology; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D018408:Patch-Clamp Techniques; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D018091:Receptors, AMPA / Q000235:genetics / Q000378:metabolism*; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D009435:Synaptic Transmission / Q000502:physiology*; D000095002:Delta Catenin
D002352:Carrier Proteins; D051177:Catenins; D015815:Cell Adhesion Molecules; C491308:Grip1 protein, rat; D047908:Intracellular Signaling Peptides and Proteins; D009419:Nerve Tissue Proteins; D010750:Phosphoproteins; D018091:Receptors, AMPA; D016194:Receptors, N-Methyl-D-Aspartate; D011993:Recombinant Fusion Proteins; C104722:glutamate receptor ionotropic, AMPA 2; D000095002:Delta Catenin