Obstetric outcomes following vitrification of in vitro and in vivo matured oocytes.
OBJECTIVE
To evaluate obstetric outcomes with oocyte vitrification after ovarian stimulation (OS) and in vitro maturation (IVM) of immature oocytes.


METHODS
A prospective trial from October 2003 to April 2007.


METHODS
University-based medical center.


METHODS
OS group: 38 patients undergoing intrauterine insemination who overresponded to OS. IVM group: 20 patients who had previous unsuccessful intrauterine insemination.


METHODS
Mature oocyte retrieval following OS. Immature oocyte retrieval and IVM. Oocyte vitrification, thawing, insemination, and transfer of the resulting embryos.


METHODS
Live-birth rates and obstetric outcomes.


RESULTS
The OS group was superior to the IVM group in terms of oocyte survival (81.4 +/- 22.6% vs. 67.5 +/- 26.1%), fertilization rate (75.6 +/- 22.5% vs. 64.2 +/- 19.9%), and cumulative embryo score (38.4 +/- 22.3 vs. 20.0 +/- 13.8). However, the differences in the implantation rate per embryo (19.1 +/- 25.8% vs. 9.6 +/- 24.1%), clinical pregnancy rate per cycle started (44.7%, vs. 20.0%), and live-birth rate per cycle started (39.5% vs. 20.0%) were not statistically significant. Twenty healthy babies were born in the OS group and four in the IVM group.


CONCLUSIONS
Pregnancies achieved with vitrification of oocytes after OS and IVM treatments do not appear to be associated with adverse pregnancy outcomes. Vitrification of IVM oocytes represents a novel option for fertility preservation.
Chian
Ri-Cheng
RC
McGill Reproductive Center, McGill University Health Centre, Montreal, Quebec, Canada.
Huang
Jack Y J
JY
Gilbert
Lucy
L
Son
Weon-Young
WY
Holzer
Hananel
H
Cui
Shan Jin
SJ
Buckett
William M
WM
Tulandi
Togas
T
Tan
Seang Lin
SL
D002470:Cell Survival; D015925:Cryopreservation / Q000379:methods*; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D007247:Infertility, Female / Q000453:epidemiology; D007248:Infertility, Male / Q000453:epidemiology; D008297:Male; D054315:Oocyte Retrieval; D009865:Oocytes / Q000166:cytology / Q000502:physiology*; D009926:Organ Preservation / Q000379:methods; D011247:Pregnancy; D011256:Pregnancy Outcome / Q000453:epidemiology; D011446:Prospective Studies