Liquid culture medium for the rapid cultivation of Helicobacter pylori from biopsy specimens.
The goal of this study was to develop a liquid culture medium for the rapid isolation, cultivation, identification and subsequent antibiotics susceptibility testing of Helicobacter pylori directly from biopsy specimens. Five liquid media were tested: Ham's F-12, Brucella broth, tryptic soybroth, brain heart infusion broth and Mueller-Hinton broth. After optimisation of the medium, it was applied in order to investigate biopsy samples from 150 patients with gastro-duodenal disorders and compared with traditional culture methods, microscopy and an H. pylori-specific TaqMan real-time polymerase chain reaction (PCR). The most reliable and rapid growth of H. pylori, even at a small inoculum size, was obtained in Ham's F-12 medium with 5% horse serum. The developed system allowed the primary isolation of H. pylori in clinical samples and provided 87% sensitivity and 100% specificity.
Sainsus
N
N
Department of Microbiology, State University of Medicine and Pharmacy N. Testemiţanu, 165, bd. Stefan cel Mare si Sfint, 2004, Chishinau, Moldova. natalia_sainsus@yahoo.com
Cattori
V
V
Lepadatu
C
C
Hofmann-Lehmann
R
R
D000293:Adolescent; D000328:Adult; D000368:Aged; D001431:Bacteriological Techniques / Q000379:methods*; D001706:Biopsy; D003470:Culture Media; D016481:Helicobacter Infections / Q000175:diagnosis*; D016480:Helicobacter pylori / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D012680:Sensitivity and Specificity
D003470:Culture Media