title: Fractionated marine invertebrate extract libraries for drug discovery.
abstract: The high-throughput screening and drug discovery paradigm has necessitated a change in preparation of natural product samples for screening programs. In an attempt to improve the quality of marine natural products samples for screening, several fractionation strategies were investigated. The final method used HP20SS as a solid support to effectively desalt extracts and fractionate the organic components. Additionally, methods to integrate an automated LCMS fractionation approach to shorten discovery time lines have been implemented.
authors.affiliation: Department of Medicinal Chemistry, University of Utah, 30 S. 2000 E. RM 307, Salt Lake City, UT 84112, USA.
authors.forename: Tim S
authors.initials: TS
authors.lastname: Bugni
authors.forename: Mary Kay
authors.initials: MK
authors.lastname: Harper
authors.forename: Malcolm W B
authors.initials: MW
authors.lastname: McCulloch
authors.forename: Jason
authors.initials: J
authors.lastname: Reppart
authors.forename: Chris M
authors.initials: CM
authors.lastname: Ireland
mesh_terms: D000818:Animals; D001688:Biological Products / Q000302:isolation & purification; D005591:Chemical Fractionation; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D007448:Invertebrates / Q000737:chemistry*; D054852:Small Molecule Libraries
chemical_list: D001688:Biological Products; D054852:Small Molecule Libraries