Kinetic analysis of bioconversion of cellulose in attrition bioreactor.
The attrition bioreactor (ABR) combines wet ball milling and enzymatic hydrolysis in one process step. It was found that the ABR did not accelerate enzyme deactivation. Interfacial forces, not shear forces, caused the most deactivation. Elimination of the air-liquid interface by covering the reactor substantially increased enzyme stability. A simple exponential kinetic model was tested to predict the cellulose conversion in an ABR. Kinetic parameters were estimated from batch runs performed at various enzyme and substrate concentrations.
Jones
E O
EO
Department of Chemical Engineering, Washington State University, Pullman, Washington 99164, USA.
Lee
J M
JM