Fluorescence measurements on functionalized polymer surfaces--problems and troubleshooting.
Plasma-chemically tailor-made polymer surfaces are of ever-increasing importance to control surface properties in material science, as well as for (bio)analytical and biomedical applications. For the characterization of such systems, sensitive fluorescence techniques are attractive tools. To underline the potential and drawbacks of these strategies, this article addresses different problems that complicate fluorometric analysis. To overcome some of these limitations, such as nonspecific adsorption of unreacted fluorescent probes, we discuss potential troubleshooting, including the use of a chromogenic and fluorogenic pyrylium dye for the detection of amino functionalities at polypropylene surfaces.
Hoffmann
Katrin
K
Federal Institute for Materials Research and Testing (BAM) I.5 Bioanalytics, Richard-Willstätter-Str. 11, D-12489 Berlin-Adlershof, Germany. katrin.hoffmann@bam.de
Mix
Renate
R
Resch-Genger
Ute
U
Friedrich
Joerg F
JF
D000327:Adsorption; D001671:Biochemistry / Q000379:methods; D001672:Biocompatible Materials / Q000737:chemistry; D006575:Heterocyclic Compounds, 3-Ring / Q000737:chemistry; D008567:Membranes, Artificial; D008956:Models, Chemical; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013053:Spectrophotometry / Q000379:methods; D013499:Surface Properties; D013997:Time Factors
D001672:Biocompatible Materials; D006575:Heterocyclic Compounds, 3-Ring; D008567:Membranes, Artificial; D011108:Polymers; C505939:pyrylium 1