title: Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor.
abstract: A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.
authors.affiliation: National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo, 162-8640, Japan.
authors.forename: Masatomo
authors.initials: M
authors.lastname: Morita
authors.forename: Makoto
authors.initials: M
authors.lastname: Ohnishi
authors.forename: Eiji
authors.initials: E
authors.lastname: Arakawa
authors.forename: N A
authors.initials: NA
authors.lastname: Bhuiyan
authors.forename: Suraia
authors.initials: S
authors.lastname: Nusrin
authors.forename: Munirul
authors.initials: M
authors.lastname: Alam
authors.forename: A K
authors.initials: AK
authors.lastname: Siddique
authors.forename: Firdausi
authors.initials: F
authors.lastname: Qadri
authors.forename: Hidemasa
authors.initials: H
authors.lastname: Izumiya
authors.forename: G Balakrish
authors.initials: GB
authors.lastname: Nair
authors.forename: Haruo
authors.initials: H
authors.lastname: Watanabe
mesh_terms: D000483:Alleles; D015373:Bacterial Typing Techniques / Q000379:methods*; D002772:Cholera Toxin / Q000235:genetics*; D017931:DNA Primers; D005240:Feasibility Studies; D005798:Genes, Bacterial / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics*
chemical_list: D017931:DNA Primers; D002772:Cholera Toxin