Alginate is not a good material for growth of rapidly proliferating cells.
BACKGROUND
Alginate scaffolds are widely used in tissue engineering. The aim of this study was to evaluate alginate as a scaffold for 3D cultures of rapidly proliferating cells.


METHODS
Murine 3T3 fibroblasts were cultured in an alginate scaffold for 30 days. Cells growing in alginate were observed under the inverted microscope. Pathologic examination by hematoxylin and eosin staining was done at the end of the experiment.


RESULTS
Migration of rapidly proliferating cells from the 3D scaffold and an inappropriate growth pattern were observed during the experiment. Cells and scaffold did not form a solid graft.


CONCLUSIONS
The results obtained in this study indicated that alginate is not a good biomaterial for a durable implant.
Pokrywczynska
M
M
Department of Tissue Engineering, Chair of Medical Biology, Collegium Medicum of Nicolaus Copernicus University, Bydgoszcz, Poland.
Drewa
T
T
Jundzill
A
A
Lysik
J
J
D016475:3T3 Cells / Q000166:cytology / Q000502:physiology; D000464:Alginates; D000818:Animals; D002455:Cell Division / Q000502:physiology*; D002465:Cell Movement; D022781:Hepatocytes / Q000166:cytology / Q000502:physiology; D007700:Kinetics; D051379:Mice; D054457:Tissue Scaffolds
D000464:Alginates