Adipose-derived stem cells: isolation, expansion and differentiation.
The emerging field of regenerative medicine will require a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue has proven to serve as an abundant, accessible and rich source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. There has been increased interest in adipose-derived stem cells (ASCs) for tissue engineering applications. Here, methods for the isolation, expansion and differentiation of ASCs are presented and described in detail. While this article has focused on the isolation of ASCs from human adipose tissue, the procedure can be applied to adipose tissues from other species with minimal modifications.
Bunnell
Bruce A
BA
Division of Gene Therapy, Tulane National Primate Research Center, Tulane University Health Sciences Center, Covington, LA 70433, USA. bbunnell@tulane.edu
Flaat
Mette
M
Gagliardi
Christine
C
Patel
Bindiya
B
Ripoll
Cynthia
C
D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism*; D000328:Adult; D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods; D002454:Cell Differentiation; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D039902:Multipotent Stem Cells / Q000166:cytology / Q000378:metabolism; D023822:Tissue Engineering / Q000379:methods