MAGE-A protein and MAGE-A10 gene expressions in liver metastasis in patients with stomach cancer.
Tumour samples from 71 patients with stomach cancer, 41 patients with liver metastasis (group A) and 15 patients each in stages II-IV (group B) and stage I (group C) without liver metastasis were analysed. MAGE-A protein expression was evaluated by immunohistochemistry using a 6C1 monoclonal antibody and MAGE-A10 mRNA expression was detected by highly sensitive in situ hybridisation using a cRNA probe. Expressions of MAGE-A protein and MAGE-A10 mRNA in group A were detected in 65.9 and 80.5%, respectively. Both protein and gene showed significantly higher expression in group A than those in groups B (6.7, 26.7%) and C (0, 0%) (P=0.0003, P=<0.0001, respectively). MAGE-A10 mRNA expression in liver metastasis was found in eight (88.9%) out of nine patients. The concordant rate between MAGE-A family protein expression and MAGE-A10 mRNA expression in the primary sites was 81.7% (P<0.0001). MAGE-A10 gene expression was associated with reduced survival duration. The results of this study suggest that MAGE-A10 is a possible target in active immunotherapy for advanced stomach cancer.
Suzuki
S
S
Department of Surgery, Tama-Nagayama Hospital, Nippon Medical School, Tama, Tokyo, Japan. seiji@nms.ac.jp
Sasajima
K
K
Sato
Y
Y
Watanabe
H
H
Matsutani
T
T
Iida
S
S
Hosone
M
M
Tsukui
T
T
Maeda
S
S
Shimizu
K
K
Tajiri
T
T
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000911:Antibodies, Monoclonal / Q000737:chemistry; D000951:Antigens, Neoplasm / Q000096:biosynthesis* / Q000235:genetics; D018450:Disease Progression; D005260:Female; D015870:Gene Expression; D006801:Humans; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000556:secondary*; D008297:Male; D058950:Melanoma-Specific Antigens; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D015347:RNA Probes; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D000509:alpha-Fetoproteins / Q000096:biosynthesis
D000911:Antibodies, Monoclonal; D000951:Antigens, Neoplasm; C119317:MAGE-A10 antigen; D058950:Melanoma-Specific Antigens; D009363:Neoplasm Proteins; D015347:RNA Probes; D012333:RNA, Messenger; D000509:alpha-Fetoproteins