Production and characterization of transgenic mice harboring mutant human UMOD gene.
Familial juvenile hyperuricemic nephropathy is caused by mutations in the UMOD gene encoding uromodulin. A transgenic mouse model was developed by introducing a human mutant UMOD (C148W) cDNA under control of the mouse umod promoter. Uromodulin accumulation was observed in the thick ascending limb cells in the kidney of transgenic mice. However, the urinary excretion of uromodulin in transgenic mice did not decrease and LC-MS/MS analysis indicated it was of mouse origin. Moreover, the creatinine clearance was not different between wildtype and transgenic animals. Consequently, the onset of the disease was not observed in transgenic mice until 24 weeks of age.
Takiue
Yuichi
Y
Department. of Pharmacotherapeutics, Kyoritsu University of Pharmacy, Tokyo, Japan.
Hosoyamada
Makoto
M
Yokoo
Takuya
T
Kimura
Masaki
M
Ochiai
Manami
M
Kaneko
Kiyoko
K
Ichida
Kimiyoshi
K
Hosoya
Tatsuo
T
Shibasaki
Toshiaki
T
D000818:Animals; D003404:Creatinine / Q000378:metabolism; D004195:Disease Models, Animal; D006801:Humans; D033461:Hyperuricemia / Q000235:genetics / Q000378:metabolism / Q000652:urine; D007668:Kidney / Q000378:metabolism / Q000473:pathology; D051379:Mice; D008822:Mice, Transgenic; D009088:Mucoproteins / Q000235:genetics* / Q000378:metabolism* / Q000652:urine; D009154:Mutation; D058949:Uromodulin
D009088:Mucoproteins; C548612:UMOD protein, human; C548613:Umod protein, mouse; D058949:Uromodulin; D003404:Creatinine