The effect of aqueous surfactant solutions on alcohol dehydrogenase (LADH).
Alcohol dehydrogenase (LADH) was studied in aqueous solutions of surfactants to determine its structural and catalytic characteristics. Fluorescence, circular dichroism (CD), and electron paramagnetic resonance (ERP) techniques were used to study structural changes to the enzyme. The activity of LADH in catalyzing the oxidation of ethanol was investigated. Short-chain alkyl sulfonates and sulfates did not deactivate LADH or alter its structure. Longer and branched alkyl sulfates and sulfonates, as well as a cationic surfactant (CTAB), affected both LADH activity and conformation.
Creagh
A L
AL
Department of Chemical Engineering, University of California, Berkeley, California 94720, USA.
Prausnitz
J M
JM
Blanch
H W
HW