Regulation of leukocyte migration across endothelial barriers by ECTO-5'-nucleotidase-generated adenosine.
CD73-deficient mice are valuable for evaluating the ability of CD73-generated adenosine to modulate adenosine receptor-mediated responses. Here we report the role of CD73 in regulating lymphocyte migration across two distinct barriers. In the first case, CD73-generated adenosine restricts the migration of lymphocytes across high endothelial venules (HEV) into draining lymph nodes after an inflammatory stimulus, apparently by triggering A(2B) receptors on HEV. Secondly, CD73 promotes the migration of pathogenic T cells into the central nervous system during experimental autoimmune encephalomyelitis. Experiments are in progress to determine whether this effect is also adenosine receptor-mediated and to identify the relevant adenosine receptor.
Thompson
Linda F
LF
Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma 73104, USA. Linda-Thompson@omrf.org
Takedachi
Masahide
M
Ebisuno
Yukihiko
Y
Tanaka
Toshiyuki
T
Miyasaka
Masayuki
M
Mills
Jeffery H
JH
Bynoe
Margaret S
MS
D015720:5'-Nucleotidase / Q000172:deficiency / Q000378:metabolism*; D000241:Adenosine / Q000096:biosynthesis* / Q000378:metabolism*; D000818:Animals; D002490:Central Nervous System / Q000166:cytology / Q000378:metabolism; D002634:Chemotaxis, Leukocyte; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000201:enzymology / Q000276:immunology / Q000378:metabolism / Q000473:pathology; D042783:Endothelial Cells / Q000378:metabolism*; D007249:Inflammation / Q000378:metabolism; D007962:Leukocytes / Q000166:cytology*; D008198:Lymph Nodes / Q000378:metabolism; D042601:Lymphatic Vessels / Q000378:metabolism; D051379:Mice; D013601:T-Lymphocytes / Q000166:cytology / Q000378:metabolism
D015720:5'-Nucleotidase; D000241:Adenosine