Controlled fed-batch fermentation of recombinant Saccharomyces cerevisiae to produce hepatitis B surface antigen.
We have performed controlled fed-batch fermentation experiments to compare the production level of hepatitis B surface antigen (HBsAg) by recombinant yeast Saccharomyces cerevisiae strains (YNN27/pYBH-1, YNN27/ p2micro-S11, YNN27/pDCB-S2) containing plasmid vector with alcohol dehydrogenase (ADH1), acid phosphatase (PHO5), and glyceraldehyde-3-phosphate dehydrogenase (GPD) promoter, respectively. Yeast cell concentrations of 15-35 g dry cell weight/L were obtained. By limiting phosphorous concentration, HBsAg expression level for the YNN27/p2micro-S11 strain with inducible PHO5 promoter reached 0.2-0.3 mg/L. By controlling nutrient addition rate and dissolved oxygen concentration, HBsAg concentrations of 3-10 mg/L were achieved in 60-70 h fermentation using the YNN27/pDCB-S2 strain with the constitutive GPD promoter.
Hsieh
J H
JH
Process Development Department, Development Center for Biotechnology, 81 Chang Hsing Street, Taipei, Taiwan, Republic of China.
Shih
K Y
KY
Kung
H F
HF
Shiang
M
M
Lee
L Y
LY
Meng
M H
MH
Chang
C C
CC
Lin
H M
HM
Shih
S C
SC
Lee
S Y
SY
Chow
T Y
TY
Feng
T Y
TY
Kuo
T T
TT
Choo
K B
KB