title: The effects of intraperitoneal sildenafil administration on healing of left colonic anastomoses and intra-abdominal adhesion formation in the presence of intra-abdominal infection.
abstract: OBJECTIVE
  The objective of this pilot study was to establish an animal model for intra-abdominal infection and to examine the effect of sildenafil on anastomotic healing of the left colon and intra-abdominal adhesion formation.
  
  
  METHODS
  Fourteen Winstar rats underwent colonic transsection and primary anastomosis after performing intra-abdominal infection. Rats were divided into two groups: Group 1 (n = 7): intra-abdominal infection, resection, and anastomoses; Group 2 (n = 7): intra-abdominal infection, resection, anastomoses, and sildenafil. Anastomotic bursting pressures, hydroxyproline levels, histopathologic grading, and abdominal adhesions were accessed on the postoperative Day 7.
  
  
  RESULTS
  Anastomotic healing was found to be improved in terms of a bursting pressure (P = 0.02). Histopathological examination revealed an increase in angiogenesis (P = 0.007). Moreover, intra-abdominal adhesions were significantly less in rats given sildenafil (P = 0.03).
  
  
  CONCLUSIONS
  Sildenafil may improve anastomotic healing of the left colon and diminishes peritoneal adhesion formation in the presense of abdominal infection.
authors.affiliation: Department of General Surgery, Faculty of Medicine, Firat University, Elazig, Turkey. refikayten@yahoo.com
authors.forename: Refik
authors.initials: R
authors.lastname: Ayten
authors.forename: Ziya
authors.initials: Z
authors.lastname: Cetinkaya
authors.forename: Mustafa
authors.initials: M
authors.lastname: Girgin
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Ozercan
authors.forename: Bilal
authors.initials: B
authors.lastname: Ustundag
authors.forename: Erhan
authors.initials: E
authors.lastname: Aygen
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000601:surgery*; D004195:Disease Models, Animal; D006909:Hydroxyproline / Q000378:metabolism; D007274:Injections, Intraperitoneal; D010538:Peritonitis / Q000378:metabolism / Q000601:surgery*; D010726:Phosphodiesterase Inhibitors / Q000008:administration & dosage*; D010865:Pilot Projects; D010879:Piperazines / Q000008:administration & dosage*; D011687:Purines / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000008:administration & dosage*; D000267:Tissue Adhesions / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000068677:Sildenafil Citrate; D006909:Hydroxyproline