title: Methods for human CD8(+) T lymphocyte proteome analysis.
abstract: T lymphocytes, including cytotoxic CD8(+) T cells, are important cells involved in immunology, as they can destroy infected or tumor cells. We describe here a detailed protocol starting from CD8(+) T lymphocytes isolation for T cell culture followed by total protein extraction or subcellular fractionation, like nuclei isolation. We also describe well-defined biochemistry and cell biology methods adapted to T lymphocytes, showing the importance of using the method best suited to answering the question addressed. All these techniques would be very helpful to immunologists willing to study underlying biological processes related to T lymphocytes.
authors.affiliation: Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland.
authors.forename: Lynne
authors.initials: L
authors.lastname: Thadikkaran
authors.forename: Nathalie
authors.initials: N
authors.lastname: Rufer
authors.forename: Corinne
authors.initials: C
authors.lastname: Benay
authors.forename: David
authors.initials: D
authors.lastname: Crettaz
authors.forename: Jean-Daniel
authors.initials: JD
authors.lastname: Tissot
mesh_terms: D015153:Blotting, Western / Q000379:methods; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry* / Q000166:cytology; D018929:Cell Culture Techniques; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D002467:Cell Nucleus / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*
chemical_list: D020543:Proteome