title: Halorubrum luteum sp. nov., isolated from Lake Chagannor, Inner Mongolia, China.
abstract: A novel halophilic archaeon, strain CGSA15(T), was isolated from water of Lake Chagannor in China. The strain grew optimally at 33-37 degrees C, pH 9.5-10.0 and 4.0-4.3 M NaCl. The major polar lipids were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. The genomic DNA G+C content of strain CGSA15(T) was 60.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CGSA15(T) was a member of the genus Halorubrum and was related most closely to Halorubrum alkaliphilum AS 1.3528(T) (96.1 % similarity) and Halorubrum tibetense AS 1.3239(T) (96.9 %). Levels of DNA-DNA relatedness between strain CGSA15(T) and Hrr. alkaliphilum AS 1.3528(T) and Hrr. tibetense AS 1.3239(T) were 36.7 and 28.9 %, respectively. According to the phenotypic and genotypic data presented, strain CGSA15(T) is considered to represent a novel species of the genus Halorubrum, for which the name Halorubrum luteum sp. nov. is proposed. The type strain is CGSA15(T) (=CGMCC 1.6783(T) =CECT 7303(T)).
authors.affiliation: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
authors.forename: Lingfei
authors.initials: L
authors.lastname: Hu
authors.forename: Hailian
authors.initials: H
authors.lastname: Pan
authors.forename: Yanfen
authors.initials: Y
authors.lastname: Xue
authors.forename: A
authors.initials: A
authors.lastname: Ventosa
authors.forename: D A
authors.initials: DA
authors.lastname: Cowan
authors.forename: B E
authors.initials: BE
authors.lastname: Jones
authors.forename: W D
authors.initials: WD
authors.lastname: Grant
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D002681:China; D005618:Fresh Water / Q000382:microbiology*; D006216:Halobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D012336:RNA, Ribosomal, 16S