Fluorescence-based methods in the study of protein-protein interactions in living cells.
Multiprotein complexes partake in nearly all cell functions, thus the characterization and visualization of protein-protein interactions in living cells constitute an important step in the study of a large array of cellular mechanisms. Recently, noninvasive fluorescence-based methods using resonance energy transfer (RET), namely bioluminescence-RET (BRET) and fluorescence-RET (FRET), and those centered on protein fragment complementation, such as bimolecular fluorescence complementation (BiFC), have been successfully used in the study of protein interactions. These new technologies are nowadays the most powerful approaches for visualizing the interactions occurring within protein complexes in living cells, thus enabling the investigation of protein behavior in their normal milieu. Here we address the individual strengths and weaknesses of these methods when applied to the study of protein-protein interactions.
Ciruela
Francisco
F
Unitat de Farmacologia, Departament de Patologia i Terapèutica Experimental, Facultat de Medicina (Campus de Bellvitge), IDIBELL-Universitat de Barcelona, 08907 L'Hospitalet del Llobregat, Barcelona, Spain. fciruela@ub.edu
D005453:Fluorescence; D031541:Fluorescence Resonance Energy Transfer; D011506:Proteins / Q000378:metabolism*
D011506:Proteins