Fourier fluorescence spectrometer for excitation emission matrix measurement.
We demonstrate a fluorescence spectrometer that utilizes principles of Fourier transform spectroscopy to measure excitation emission matrices (EEM) rapidly and with high spectral resolution. For this EEM fluorometer, incoherent excitation light is first input into a differential-delay scanning Michelson interferometer. Light from the output port excites sample fluorescence. The fluorescence remitted from the sample is directed to a second Michelson interferometer, whose differential-delay scanning is synchronized with the first interferometer. The EEM is obtained by two-dimensional Fourier analysis of the detected signal from the output port of the second interferometer. EEM results from the system are verified by comparing with results from a standard spectrometer. The system provides a wide spectral range, adjustable spectral resolution, and fast EEM acquisition speed, which allows EEM's to be acquired in 40 seconds at a spectral resolution of 81-cm-1.
Peng
Leilei
L
Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 55 Fruit Street,BAR 708, Massachusetts 02114, USA. lpeng1@partners.org
Gardecki
Joseph A
JA
Bouma
Brett E
BE
Tearney
Guillermo J
GJ
D004867:Equipment Design; D005453:Fluorescence; D005583:Fourier Analysis; D007091:Image Processing, Computer-Assisted; D007368:Interferometry / Q000295:instrumentation / Q000379:methods; D007834:Lasers; D008027:Light; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013997:Time Factors