Comprehensive two-dimensional high-performance liquid chromatography system with immobilized liposome chromatography column and reversed-phase column for separation of complex traditional Chinese medicine Longdan Xiegan Decoction.
A comprehensive two-dimensional HPLC system with an immobilized liposome chromatography (ILC) column in conjunction with an RP column (in tandem) was developed for the screening and analysis of the membrane-permeable compounds in a traditional Chinese medicine prescription Longdan Xiegan Decoction (LXD). More than 50 components in LXD were resolved using the developed separation system. Eight flavonoids and two iridoids were identified interacting with the ILC column; a system that mimics biomembranes. The results show that the developed comprehensive two-dimensional chromatography system can be used for identifying membrane permeable natural products in complex matrixes such as extracts of traditional Chinese medicine prescriptions.
Wang
Yun
Y
Key Laboratory of Standardization of Chinese Medicines of Ministry of Education, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China.
Kong
Liang
L
Lei
Xiaoyuan
X
Hu
Lianghai
L
Zou
Hanfa
H
Welbeck
Ed
E
Bligh
S W Annie
SW
Wang
Zhengtao
Z
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D005419:Flavonoids / Q000032:analysis*; D039823:Iridoids / Q000032:analysis*; D008081:Liposomes; D013058:Mass Spectrometry; D008567:Membranes, Artificial
D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D039823:Iridoids; D008081:Liposomes; D008567:Membranes, Artificial; C526720:longdan xiegan decoction