Molecular cloning and characterization of the Dicer-like 2 gene from Brassica rapa.
Dicer-like proteins (DCLs) are involved in small RNA-mediated development and viral defense in plants. In model plants, at least four DCLs have been found and a number of studies have helped to understand their function. However, the function of the Dicer or DCLs in other plants is still unclear. Here, we report the full-length cDNA sequence of Brassica rapa ssp. chinensis DCL2 (BrDCL2) gene, which contains a 4,179 bp open reading frame (ORF) encoding a protein of 1,392 amino acids. At the 3' end of BrDCL2, clones with three different lengths of 3' untranslated region were found. An alternative splice variant of BrDCL2, BrDCL2sv, in which one intron was retained between exon9 and exon10, was also cloned. Because of a change in the coding sequence resulting in a premature terminal codon, BrDCL2sv was expected to translate a short peptide containing the whole DEXHc domain.
Yan
Fei
F
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
Peng
Jiejun
J
Lu
Yuwen
Y
Lin
Lin
L
Zheng
Hongying
H
Chen
Hairu
H
Chen
Jianping
J
Adams
Michael J
MJ
D020413:3' Untranslated Regions; D001483:Base Sequence; D029741:Brassica rapa / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics; D020033:Protein Isoforms; D043244:Ribonuclease III / Q000235:genetics*
D020413:3' Untranslated Regions; D018076:DNA, Complementary; D010940:Plant Proteins; D020033:Protein Isoforms; D043244:Ribonuclease III