title: Rapid culture-free identification and molecular typing of Shiga toxin-producing Escherichia coli by PCR-RFLP.
abstract: Using culture-independent technology, PCR-RFLP were used to identify and type STEC in the stool of a patient with HUS. Fecal PCR-RFLP patterns were identical to those of the STEC O157:H7 isolated from the patient.
authors.affiliation: Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Naka-ku, Sakai, Osaka, Japan.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Shima
authors.forename: Naohisa
authors.initials: N
authors.lastname: Kawamura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hinenoya
authors.forename: Norihiko
authors.initials: N
authors.lastname: Sugimoto
authors.forename: Yuluo
authors.initials: Y
authors.lastname: Wu
authors.forename: Masahiro
authors.initials: M
authors.lastname: Asakura
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Nishimura
authors.forename: Gopinath B
authors.initials: GB
authors.lastname: Nair
authors.forename: Shinji
authors.initials: S
authors.lastname: Yamasaki
mesh_terms: D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003937:Diagnosis, Differential; D003967:Diarrhea / Q000175:diagnosis* / Q000382:microbiology; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D005240:Feasibility Studies; D005243:Feces / Q000382:microbiology*; D006463:Hemolytic-Uremic Syndrome / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
chemical_list: D004269:DNA, Bacterial