Fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay based on the aggregation-induced emission of silole.
By making use of the aggregation-induced emission feature of silole, compound 1 with an ammonium group is designed and synthesized with a view to developing a new optical probe for fluorescence turn-on detection of DNA and label-free fluorescence nuclease assay. The fluorescence of 1 increases largely upon mixing with DNA, in particular for long DNA, indicating that 1 can be used for fluorescence turn-on detection of DNA. More interestingly, 1 can be employed to follow the DNA cleavage process by nuclease. Therefore, a label-free fluorescence nuclease assay method is successfully established with 1. Furthermore, this label-free fluorescence assay can also be used for inhibitor screening of nucleases. Given its simplicity, easy operation, sensitivity and cost-effectiveness, this method can be extended to other nuclease assays and high-throughput screening of nuclease inhibitors.
Wang
Ming
M
Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.
Zhang
Deqing
D
Zhang
Guanxin
G
Tang
Yanli
Y
Wang
Shu
S
Zhu
Daoben
D
D001681:Biological Assay; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D005453:Fluorescence; D007202:Indicators and Reagents / Q000737:chemistry*; D017646:Organosilicon Compounds / Q000138:chemical synthesis / Q000737:chemistry*; D015719:Single-Strand Specific DNA and RNA Endonucleases / Q000032:analysis / Q000378:metabolism*
D007202:Indicators and Reagents; D017646:Organosilicon Compounds; D004247:DNA; D005090:Exodeoxyribonucleases; C028450:exodeoxyribonuclease I; D015719:Single-Strand Specific DNA and RNA Endonucleases