title: Spontaneous and BSE-prion-seeded amyloid formation of full length recombinant bovine prion protein.
abstract: The conversion of the cellular isoform of the prion protein into the pathogenic isoform PrP(Sc) is the key event in prion diseases. The disease can occur spontaneously genetically or by infection. In earlier studies we presented an in vitro conversion system which simulates the structural transition in recPrP by varying low concentrations of SDS at constant NaCl. In the present study we adopted the conversion system from experimental Scrapie in hamster to bovine recPrP and generated amyloid fibrils. The intermediate state which is optimal for fibril formation is a soluble, beta-rich state. The system was extended using BSE-prions as seeds and led to an acceleration of fibril formation by orders of magnitude. This seeded amyloid formation assay avoids any PK-treatment, is therefore able to detect even PK-sensitive PrP(Sc) and does not require cellular components.
authors.affiliation: Institut fuer Physikalische Biologie and Biologisch-Medizinisches Forschungszentrum, Heinrich-Heine-Universitaet Duesseldorf, Universitiätsstr. 1, 40225 Duesseldorf, Germany.
authors.forename: Giannantonio
authors.initials: G
authors.lastname: Panza
authors.forename: Jan
authors.initials: J
authors.lastname: Stöhr
authors.forename: Christian
authors.initials: C
authors.lastname: Dumpitak
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Papathanassiou
authors.forename: Jürgen
authors.initials: J
authors.lastname: Weiss
authors.forename: Detlev
authors.initials: D
authors.lastname: Riesner
authors.forename: Dieter
authors.initials: D
authors.lastname: Willbold
authors.forename: Eva
authors.initials: E
authors.lastname: Birkmann
mesh_terms: D000682:Amyloid / Q000096:biosynthesis* / Q000737:chemistry; D000818:Animals; D002417:Cattle; D006224:Cricetinae; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism*; D008958:Models, Molecular; D018620:PrPSc Proteins / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000682:Amyloid; D018620:PrPSc Proteins; D011994:Recombinant Proteins