title: [Current directions of studies for development of methods for specific diagnostics of allergic diseases in vitro].
abstract: Results of studies as well as perspectives for development and use of modern in vitro methods of allergic diseases diagnostics are presented. High prevalence of allergic diseases dictates the need of high-quality etiologic diagnosis for conducting relevant etiopathogenetic treatment - allergen-specific immunotherapy and prophylactic measures. Interest to in vitro diagnostic methods due to their high specificity and commonality is rising. They are directed to identification of various elements of "material substrate" of allergic reaction - specific antibodies, cell elements, cytokines as well as genes, which encode them. Intensively developing methods based on immunoenzyme, immunofluorescent, and immunochemiluminescent reactions are widely used now. Methods based on the use of various monoclonal antibodies for identification of lymphocytic markers, cytokines, antigen-presenting cells by laser flowcytometry are also very promising. ELISPOT technology is also intensively used for diagnostics of allergic diseases. From immunogenetic methods great potential belongs to molecular biologic methods of assessment of genome specificity characterizing sensitization to given antigen.
authors.forename: B
authors.initials: B
authors.lastname: Petrunov
mesh_terms: D000485:Allergens / Q000032:analysis / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000276:immunology; D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D000941:Antigens / Q000032:analysis / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis / Q000235:genetics; D003888:Desensitization, Immunologic; D005455:Fluorescent Antibody Technique / Q000379:methods; D006801:Humans; D006967:Hypersensitivity / Q000175:diagnosis* / Q000276:immunology / Q000628:therapy; D007114:Immunization; D007120:Immunochemistry / Q000379:methods; D007124:Immunoenzyme Techniques / Q000379:methods; D049449:Luminescence
chemical_list: D000485:Allergens; D000906:Antibodies; D000911:Antibodies, Monoclonal; D000941:Antigens; D016207:Cytokines