title: A self-renewing, tissue-engineered vascular graft for arterial reconstruction.
abstract: OBJECTIVE
  Various tissue-engineered vascular grafts have been studied to overcome the clinical disadvantages of conventional prostheses. Previous tissue-engineered vascular grafts have generally required preoperative cellular manipulation or use of bioreactors to improve performance, and their mechanical properties have been insufficient. We focused on the concept of in situ cellularization and developed a tissue-engineered vascular graft for arterial reconstruction that would facilitate renewal of autologous tissue without any pretreatment.
  
  
  METHODS
  The graft comprised an interior of knitted polyglycolic acid compounded with collagen to supply a scaffold for tissue growth and an exterior of woven poly-L-lactic acid for reinforcement. All components were biocompatible and biodegradable, with excellent cellular affinity. The grafts, measuring 10 mm in internal diameter and 30 mm in length, were implanted into porcine aortas, and their utility was evaluated to 12 months after grafting.
  
  
  RESULTS
  All explants were patent throughout the observation period, with no sign of thrombus formation or aneurysmal change. Presence in the neomedia of endothelialization with proper integrity and parallel accumulation of functioning smooth muscle cells, which responded to vasoreactive agents, was confirmed in an early phase after implantation. Sufficient collagen synthesis and lack of elastin were quantitatively demonstrated. Dynamic assessment and long-term results of the in vivo study indicated adequate durability of the implants.
  
  
  CONCLUSIONS
  The graft showed morphologic evidence of good in situ cellularization, satisfactory durability to withstand arterial pressure for 12 postoperative months, and the potential to acquire physiologic vasomotor responsiveness. These results suggest that our tissue-engineered vascular graft shows promise as an arterial conduit prosthesis.
authors.affiliation: Division of Cardiovascular Surgery, Department of Surgery, Osaka University Graduate School of Medicine, Osaka, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Torikai
authors.forename: Hajime
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Koichiro
authors.initials: K
authors.lastname: Hirakawa
authors.forename: Goro
authors.initials: G
authors.lastname: Matsumiya
authors.forename: Toru
authors.initials: T
authors.lastname: Kuratani
authors.forename: Shigemitsu
authors.initials: S
authors.lastname: Iwai
authors.forename: Atsuhiro
authors.initials: A
authors.lastname: Saito
authors.forename: Naomasa
authors.initials: N
authors.lastname: Kawaguchi
authors.forename: Nariaki
authors.initials: N
authors.lastname: Matsuura
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Sawa
mesh_terms: D020341:Absorbable Implants; D000818:Animals; D001013:Aorta, Thoracic / Q000601:surgery*; D001158:Arteries; D001672:Biocompatible Materials; D001705:Bioprosthesis; D001807:Blood Vessel Prosthesis; D019917:Blood Vessel Prosthesis Implantation; D002478:Cells, Cultured; D003094:Collagen / Q000737:chemistry; D004730:Endothelium, Vascular / Q000637:transplantation* / Q000648:ultrastructure; D005347:Fibroblasts / Q000637:transplantation; D008422:Materials Testing; D051379:Mice; D009131:Muscle, Smooth, Vascular; D011100:Polyglycolic Acid / Q000737:chemistry; D011138:Polytetrafluoroethylene; D011474:Prosthesis Design; D013552:Swine; D013556:Swine, Miniature; D013718:Tensile Strength; D023822:Tissue Engineering; D014471:Umbilical Veins / Q000637:transplantation
chemical_list: D001672:Biocompatible Materials; D011100:Polyglycolic Acid; D011138:Polytetrafluoroethylene; D003094:Collagen