title: Optimization of batch fermentor sterilization.
abstract: This article presents a calculation procedure useful for the optimization and scale up of batch sterilization cycles in large-scale fermentors. This technique determines the sterilization temperature and hold-time necessary to minimize nutrient damage in a specific fermentor. The method can also be used for "scaledown" experiments to eliminate sterilization conditions as a scale up parameter. A method for the systematic evaluation of different sterilization conditions on product yield is also presented. This procedure is useful in determining if scale up of sterilization conditions is important for a given process. The validity of the techniques presented are supported by data showing significant yield improvements in a 1.2 x 10(5) L antibiotic fermentation.
authors.affiliation: Schering Corporation, 1011 Morris Ave, Union, New Jersey 07083, USA.
authors.forename: V
authors.initials: V
authors.lastname: Singh
authors.forename: W
authors.initials: W
authors.lastname: Hensler
authors.forename: R
authors.initials: R
authors.lastname: Fuchs