[Purification of total flavonoid glycosides from Hypericum perforatum by macroporous adsorption resin with on-line detection system].
OBJECTIVE
To design a macroporous resin chromatography system with on-line detector which can be used to purify total flavonoid glycosides from Hypericum perforatum.


METHODS
Compared with the present detection methods, the ultraviolet detector which can be applied to flow liquid, and a self-made shunt were applied to design the on-line detection system. This system was successfully applied to purify the total flavonoid glycosides from H. perforatum.


RESULTS
This on-line detection system was simple and feasible, and the results were accurate and reliable. The purity of total flavonoid glycosides from H. perforatum. separated by this system was higher than 90%.


CONCLUSIONS
This technique provides a simple and reliable on-line detection method for industrial chromatography purification of TCM extracts, which is the basis of automatic manufactory of TCM purification.
Zhao
Ming-Bo
MB
School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing 100083, China.
Jiang
Yong
Y
Zhang
Hong-Quan
HQ
Tu
Peng-Fei
PF
D000327:Adsorption; D002845:Chromatography; D005419:Flavonoids / Q000737:chemistry*; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification*; D020902:Hypericum / Q000737:chemistry*; D009862:Online Systems; D016062:Porosity; D011786:Quality Control; D015203:Reproducibility of Results; D012116:Resins, Plant / Q000737:chemistry*; D012680:Sensitivity and Specificity; D014466:Ultraviolet Rays
D005419:Flavonoids; D006027:Glycosides; D012116:Resins, Plant