Oncostatin M induces functional and structural impairment of blood-brain barriers comprised of rat brain capillary endothelial cells.
Oncostatin M (OSM), a member of the interleukin-6 family, is produced by monocytes and macrophages in the peripheral blood and microglia in the brain. The present study aimed to elucidate a regulatory role of OSM in the functions of blood-brain barrier (BBB) comprised of rat brain capillary endothelial cells (RBECs). OSM decreased the transendothelial electrical resistance of RBEC monolayers in a concentration- and time-dependent manner. Immunocytochemical observations of ZO-1 and claudin-5 in OSM-treated RBECs showed a zipper-like and/or zigzag shape along the junctions between cells, in contrast with the smooth and linear shape in vehicle-treated cultures. When RBECs were pre-treated with anti-OSM antibody, OSM failed to evoke these changes. The cellular constituents producing OSM in the brain and peripheral blood could be implicated in the functional and structural impairment of the BBB.
Takata
Fuyuko
F
Department of Pharmaceutical Care and Health Sciences, Faculty of Pharmaceutical Sciences, Fukuoka University, 8-19-1 Nanakuma, Fukuoka 814-0180, Japan.
Sumi
Noriko
N
Nishioku
Tsuyoshi
T
Harada
Eriko
E
Wakigawa
Tomoya
T
Shuto
Hideki
H
Yamauchi
Atsushi
A
Kataoka
Yasufumi
Y
D000818:Animals; D000906:Antibodies / Q000494:pharmacology; D001812:Blood-Brain Barrier / Q000166:cytology*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D017097:Electric Impedance; D042783:Endothelial Cells / Q000187:drug effects* / Q000473:pathology; D006131:Growth Inhibitors / Q000276:immunology / Q000633:toxicity*; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D053683:Oncostatin M / Q000276:immunology / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors
D000906:Antibodies; D006131:Growth Inhibitors; D008565:Membrane Proteins; D053683:Oncostatin M