Cytotoxic effect of drugs eluted from polymethylmethacrylate on stromal giant-cell tumour cells: an in vitro study.
Curettage and packing with polymethylmethacrylate cement is a routine treatment for giant-cell tumour (GCT) of bone. We performed an in vitro evaluation of the cytotoxic effect of a combination of cement and methotrexate, doxorubicin and cisplatin on primary cell cultures of stromal GCT cells obtained from five patients. Cement cylinders containing four different concentrations of each drug were prepared, and the effect of the eluted drugs was examined at three different time intervals. We found that the cytotoxic effect of eluted drugs depended on their concentration and the time interval, with even the lowest dose of each drug demonstrating an acceptable rate of cytotoxicity. Even in low doses, cytotoxic drugs mixed with polymethylmethacrylate cement could therefore be considered as effective local adjuvant treatment for GCTs.
Savadkoohi
D Gouran
DG
Pasteur Institute of Iran, Tehran, Iran.
Sadeghipour
P
P
Attarian
H
H
Sardari
S
S
Eslamifar
A
A
Shokrgozar
M A
MA
D000328:Adult; D001843:Bone Cements / Q000627:therapeutic use*; D001859:Bone Neoplasms / Q000188:drug therapy* / Q000473:pathology; D017024:Chemotherapy, Adjuvant / Q000379:methods; D003475:Curettage / Q000379:methods; D004305:Dose-Response Relationship, Drug; D016503:Drug Delivery Systems; D005260:Female; D018212:Giant Cell Tumor of Bone / Q000188:drug therapy* / Q000473:pathology; D006801:Humans; D008297:Male; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000517:prevention & control; D010006:Osteoblasts / Q000187:drug effects; D019904:Polymethyl Methacrylate / Q000627:therapeutic use*; D017154:Stromal Cells / Q000187:drug effects; D016896:Treatment Outcome
D001843:Bone Cements; D019904:Polymethyl Methacrylate