Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus.
For investigating the feasibility of using disperse dyes as an immunoassay chromogenic marker, a disperse dye, DADISPERSE NAVY BLUE SP, was selected in analyzing antibody against infectious bursal disease virus (anti-IBDV). With the color intensity revealed in the disperse dye immunochromatographic test (DICT) strip as the objective function, the optimal dyeing conditions were found as follows: dye concentration absorbance (at lambda(max)=587nm)=3, pH 7, 50 degrees C, for 10min. Under these conditions, the resultant dyed-antibody (rabbit anti-chicken) can produce an optimal color intensity reading of 55,054 on the strip. For performing qualitative immunoassay, chicken sera samples taken from different farms were used for the anti-IBDV titre assessment. The results of DICT strips showed very high sensitivity and specificity as compared to that analyzed by FlockChek enzyme linked immunosorbent assay (F-ELISA) kits. For quantitative immunoassay, it was found that the color intensity measured with DICT was linearly correlated to that of F-ELISA titre (r(2)=0.9687). Therefore, DICT was further applied to the detection of chicken anti-IBDV sera under vaccination in the farms. The average titres of the sampling groups exhibited a strong agreement to that of F-ELISA. Accordingly, the DICT method developed in this study, shown to be reliable, cheap and simple in both qualitative and quantitative immunoassays, is particularly suitable for point-of-need testing (PONT) in agricultural applications.
Wang
Shwu-Jinng
SJ
Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, ROC.
Chang
Wei-Feng
WF
Wang
Min-Ying
MY
Hsiung
Kuang-Pin
KP
Liu
Yung-Chuan
YC
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D018175:Birnaviridae Infections / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary* / Q000821:virology; D002645:Chickens; D004396:Coloring Agents / Q000737:chemistry*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D007243:Infectious bursal disease virus / Q000276:immunology*; D011201:Poultry Diseases / Q000175:diagnosis / Q000276:immunology / Q000821:virology*; D011934:Reagent Strips / Q000737:chemistry*; D012680:Sensitivity and Specificity
D000914:Antibodies, Viral; D004396:Coloring Agents; D011934:Reagent Strips