Corneal pathology in microphthalmia with linear skin defects syndrome.
To describe the histopathology of the cornea in microphthalmia with linear streaks (MLS) syndrome.



Two patients with MLS syndrome underwent penetrating keratoplasty. This study describes the histopathology and investigates immunophenotype of the corneal extracellular matrix by using keratan sulfate and collagen type III antibodies.



Clinical examination revealed bilateral sclerocornea and characteristic skin changes. By light microscopy, central corneal stroma in both patients showed vascularization and irregular thick collagen lamellae typical of sclerocornea. In addition, corneal thinning, anterior synechiae, and the absence of the Descemet membrane were noted, which was suggestive of Peters anomaly. Diffuse and intense anti-keratan sulfate staining and minimal anti-collagen type III stromal staining were seen in both corneal buttons.



The cornea in MLS may clinically resemble sclerocornea. Histologic features resemble those previously described in sclerocornea and also seen in anterior segment dysgeneses. Keratan sulfate and collagen type III labeling suggests that the corneal extracellular matrix resembled cornea and not sclera.
Kapur
Rashmi
R
Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, USA.
Tu
Elmer Y
EY
Toyran
Sami
S
Shah
Parthiv
P
Vangveeravong
Sumalee
S
Lloyd
William C
WC
Edward
Deepak P
DP
D024061:Collagen Type III / Q000378:metabolism; D003315:Cornea / Q000002:abnormalities* / Q000378:metabolism / Q000473:pathology*; D003318:Corneal Opacity / Q000150:complications* / Q000235:genetics / Q000601:surgery; D005109:Extracellular Matrix / Q000378:metabolism; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D007632:Keratan Sulfate / Q000378:metabolism; D015948:Keratoplasty, Penetrating; D008297:Male; D008850:Microphthalmos / Q000150:complications* / Q000235:genetics; D012590:Sclera / Q000002:abnormalities* / Q000378:metabolism; D012868:Skin Abnormalities / Q000150:complications* / Q000235:genetics; D013577:Syndrome
D024061:Collagen Type III; D007632:Keratan Sulfate