title: No evidence for isolated imprinting mutations in the PEG1/MEST locus in Silver-Russell patients.
abstract: Imprinting defects have meanwhile been described in nearly all human imprinting disorders among them Silver-Russell syndrome (SRS). In this disorder, 11p15 epimutations and maternal Uniparental Disomy of chromosome 7 (UPD7) are detectable in approximately 50% of patients. To find out whether isolated imprinting defects on chromosome 7 play a role in the aetiology of SRS we screened a cohort of 54 SRS patients without 11p15 epimutations. Methylation-specific PCR was carried out for the PEG1/MEST locus in 7q31. This test detects all known segmental and complete UPD7 cases. The exclusion of isolated imprinting defects in our study population shows that this type of epimutation at the PEG1/MEST locus in 7q31 does not play a relevant role in SRS. However, the role of imprinting disturbances in other genes cannot be excluded.
authors.affiliation: Institute of Human Genetics, RWTH Aachen, University Hospital, Pauwelsstrasse 30, D-52074 Aachen, Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Schöherr
authors.forename: Susanne
authors.initials: S
authors.lastname: Jäger
authors.forename: Michael B
authors.initials: MB
authors.lastname: Ranke
authors.forename: Hartmut A
authors.initials: HA
authors.lastname: Wollmann
authors.forename: Gerhard
authors.initials: G
authors.lastname: Binder
authors.forename: Thomas
authors.initials: T
authors.lastname: Eggermann
mesh_terms: D002897:Chromosomes, Human, Pair 7 / Q000235:genetics; D019066:Facies; D005260:Female; D005317:Fetal Growth Retardation / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D018392:Genomic Imprinting / Q000235:genetics*; D006801:Humans; D009154:Mutation; D011247:Pregnancy; D011506:Proteins / Q000235:genetics*; D012886:Skull / Q000002:abnormalities; D013577:Syndrome; D024182:Uniparental Disomy / Q000235:genetics
chemical_list: D005819:Genetic Markers; D011506:Proteins; C103637:mesoderm specific transcript protein