title: Antibody responses to the merozoite surface protein-1 complex in cerebral malaria patients in India.
abstract: BACKGROUND
  Plasmodium falciparum infection causes cerebral malaria (CM) in a subset of patients with anti-malarial treatment protecting only about 70% to 80% of patients. Why a subset of malaria patients develops CM complications, including neurological sequelae or death, is still not well understood. It is believed that host immune factors may modulate CM outcomes and there is substantial evidence that cellular immune factors, such as cytokines, play an important role in this process. In this study, the potential relationship between the antibody responses to the merozoite surface protein (MSP)-1 complex (which consists of four fragments namely: MSP-1(83), MSP-1(30), MSP-1(38) and MSP-1(42)), MSP-6(36) and MSP-7(22) and CM was investigated.
  
  
  METHODS
  Peripheral blood antibody responses to recombinant antigens of the two major allelic forms of MSP-1 complex, MSP-6(36) and MSP-7(22) were compared between healthy subjects, mild malaria patients (MM) and CM patients residing in a malaria endemic region of central India. Total IgG and IgG subclass antibody responses were determined using ELISA method.
  
  
  RESULTS
  The prevalence and levels of IgG and its subclasses in the plasma varied for each antigen. In general, the prevalence of total IgG, IgG1 and IgG3 was higher in the MM patients and lower in CM patients compared to healthy controls. Significantly lower levels of total IgG antibodies to the MSP-1(f38), IgG1 levels to MSP-1(d83), MSP-1(19) and MSP-6(36) and IgG3 levels to MSP-1(f42) and MSP-7(22) were observed in CM patients as compared to MM patients.
  
  
  CONCLUSIONS
  These results suggest that there may be some dysregulation in the generation of antibody responses to some MSP antigens in CM patients and it is worth investigating further whether perturbations of antibody responses in CM patients contribute to pathogenesis.
authors.affiliation: Malaria Branch, Division of Parasitic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Coordinating Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA. frd9@cdc.gov
authors.forename: Naomi W
authors.initials: NW
authors.lastname: Lucchi
authors.forename: Jon Eric
authors.initials: JE
authors.lastname: Tongren
authors.forename: Vidhan
authors.initials: V
authors.lastname: Jain
authors.forename: Avinash C
authors.initials: AC
authors.lastname: Nagpal
authors.forename: Christian W
authors.initials: CW
authors.lastname: Kauth
authors.forename: Ute
authors.initials: U
authors.lastname: Woehlbier
authors.forename: Hermann
authors.initials: H
authors.lastname: Bujard
authors.forename: Aditya P
authors.initials: AP
authors.lastname: Dash
authors.forename: Neeru
authors.initials: N
authors.lastname: Singh
authors.forename: Jonathan K
authors.initials: JK
authors.lastname: Stiles
authors.forename: Venkatachalam
authors.initials: V
authors.lastname: Udhayakumar
mesh_terms: D000328:Adult; D000818:Animals; D000913:Antibodies, Protozoan / Q000097:blood*; D000953:Antigens, Protozoan; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D007194:India; D016779:Malaria, Cerebral / Q000276:immunology* / Q000469:parasitology; D016778:Malaria, Falciparum / Q000276:immunology*; D008297:Male; D020066:Merozoite Surface Protein 1 / Q000276:immunology*; D010963:Plasmodium falciparum / Q000276:immunology*; D011994:Recombinant Proteins
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D007074:Immunoglobulin G; D020066:Merozoite Surface Protein 1; D011994:Recombinant Proteins