title: Direct evidence that ThiI is an ATP pyrophosphatase for the adenylation of uridine in 4-thiouridine biosynthesis.
authors.affiliation: Laboratory of Microbial Metabolism and College of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai 200030, China. dlyou@sjtu.edu.cn
authors.forename: Delin
authors.initials: D
authors.lastname: You
authors.forename: Tiegang
authors.initials: T
authors.lastname: Xu
authors.forename: Fen
authors.initials: F
authors.lastname: Yao
authors.forename: Xiufen
authors.initials: X
authors.lastname: Zhou
authors.forename: Zixin
authors.initials: Z
authors.lastname: Deng
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D007700:Kinetics; D011499:Protein Processing, Post-Translational; D011755:Pyrophosphatases / Q000378:metabolism*; D021241:Spectrometry, Mass, Electrospray Ionization; D013379:Substrate Specificity; D013466:Sulfurtransferases / Q000378:metabolism*; D013891:Thiouridine / Q000378:metabolism*; D014529:Uridine / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D013891:Thiouridine; D000255:Adenosine Triphosphate; D013466:Sulfurtransferases; C107110:ThiI protein, bacteria; D011755:Pyrophosphatases; D014529:Uridine