title: [Research on the application of supercritical fluid extraction of diosgenin of Smilax china].
abstract: OBJECTIVE
  To Study the optimum extraction conditions of diosgenin by CO2 Supercritical Fluid Extraction ( SFE).
  
  
  METHODS
  Uniform design was used to select the technology of CO2 SFE. The pressure, temperature and modifer concentration were discussed to try to find out the best condition. Samples were analyzed with a reverse phase HPLC system to calculate the yield. Data was processed by SPSS statistical analysis software.
  
  
  RESULTS
  The best extraction condition in SFE as follows: extraction pressure 25 MPa, temperature 67 degrees C, seperation pressure 10 MPa, modifer concentration 95%.
  
  
  CONCLUSIONS
  CO2 SFE method acquires more yield than that of traditional organic solvent extraction method in pharmacopoeia, providing scientific support on improving the extraction method of diosgenin.
authors.affiliation: The Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: Dong-sheng
authors.initials: DS
authors.lastname: Chen
authors.forename: Xiao-li
authors.initials: XL
authors.lastname: Hua
authors.forename: Li-xiu
authors.initials: LX
authors.lastname: Yu
mesh_terms: D002245:Carbon Dioxide; D002681:China; D002851:Chromatography, High Pressure Liquid; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004144:Diosgenin / Q000032:analysis / Q000302:isolation & purification*; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D032281:Smilax / Q000737:chemistry*; D013678:Technology, Pharmaceutical / Q000379:methods; D013696:Temperature
chemical_list: D002245:Carbon Dioxide; D004144:Diosgenin