[Study on processing for Rhizoma Pinelliae Praeparatum by comprehensive evaluation of multiple mark].
OBJECTIVE
To investigate the best processing technology of Rhizoma Pinelliae Praeparatum.


METHODS
L9(3(4)) orthogonal design was used with four factors: quantities of quick lime and Glycyrrhiza urallensis, processing time and temperature, the contents of Calcium oxalate crystals, Guanosine and Glycyrrhizic acid were determined by RP-HPLC, the irritation test was detected by swollen rabbits eyes with processing products.


RESULTS
The optimized processing technology was satisfied with some conditions as follows: the processing time was 48 hours, the processing temperature was 30 degrees C, quantities of quick lime and Glycyrrhiza uralensis were 10 g and 15 g.


CONCLUSIONS
The optimized processing method by orthogonal design has achieved the goal to reduce toxicity, the processing time and the processing temperature are confirmed respectively, and the processing time is significantly shortened comparing with Pharmacopoeia.
Zhang
Lin
L
Nanjing University of Chinese Medicine, Nanjing 210029, China. juezhangyan@gmail.com
Wu
Hao
H
Zhu
Tao
T
D000818:Animals; D002129:Calcium Oxalate / Q000032:analysis* / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003228:Conjunctiva / Q000187:drug effects / Q000473:pathology; D003229:Conjunctival Diseases / Q000139:chemically induced; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000633:toxicity; D005260:Female; D019695:Glycyrrhizic Acid / Q000032:analysis; D006151:Guanosine / Q000032:analysis*; D008297:Male; D030021:Pinellia / Q000737:chemistry* / Q000633:toxicity; D035281:Plant Tubers / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011817:Rabbits; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature
D004365:Drugs, Chinese Herbal; D006151:Guanosine; D002129:Calcium Oxalate; D019695:Glycyrrhizic Acid