title: Evaluation of a fluorescence polarization assay for the detection of serum antibodies to Brucella abortus in water buffalo (Bubalus bubalis).
abstract: The fluorescence polarization assay (FPA) was evaluated for the serological diagnosis of brucellosis in water buffalo (Bubalus bubalis) in southern Italy. This assay uses O-polysaccharide prepared from Brucella abortus lipopolysaccharide conjugated with fluorescein isothiocyanate as a tracer. It has many methodological advantages over older, more established tests and can be performed in a fraction of the time. Sera from 890 buffalos from the Campania Region - 526 positive sera and 364 negative sera according to the complement fixation test (CFT) - were evaluated in this study. All samples were tested with the Rose Bengal test (RBT), CFT, and FPA in parallel and in blind fashion. Sensitivities (Sn) were 84.5% and 92.6%, and specificities (Sp) were 93.1% and 91.2% for RBT and FPA, respectively, relative to CFT. Finally, receiver operating characteristic (ROC) analysis suggested a cut-off value of 117 millipolarization (mP) units. On the whole, these results suggested that FPA might replace RBT in the diagnosis of buffalo brucellosis for its better performance relative to CFT, its adjustable cut-off useful in different epidemiological situations, its reliability, ease of performance, and for its potential application in field and high-throughput laboratories.
authors.affiliation: Department of Pathology and Animal Health, University of Naples Federico II, Via Delpino no. 1, 80137 Naples, Italy. semontag@unina.it
authors.forename: S
authors.initials: S
authors.lastname: Montagnaro
authors.forename: M
authors.initials: M
authors.lastname: Longo
authors.forename: K
authors.initials: K
authors.lastname: Mallardo
authors.forename: G
authors.initials: G
authors.lastname: Pisanelli
authors.forename: L
authors.initials: L
authors.lastname: De Martino
authors.forename: G
authors.initials: G
authors.lastname: Fusco
authors.forename: L
authors.initials: L
authors.lastname: Baldi
authors.forename: U
authors.initials: U
authors.lastname: Pagnini
authors.forename: G
authors.initials: G
authors.lastname: Iovane
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D002003:Brucella abortus / Q000276:immunology / Q000302:isolation & purification*; D002006:Brucellosis / Q000097:blood / Q000382:microbiology / Q000662:veterinary*; D002020:Buffaloes / Q000097:blood* / Q000382:microbiology*; D003168:Complement Fixation Tests / Q000662:veterinary; D016231:Fluorescence Polarization Immunoassay / Q000379:methods / Q000592:standards / Q000662:veterinary*; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D012372:ROC Curve; D012395:Rose Bengal / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D000907:Antibodies, Bacterial; D011135:Polysaccharides, Bacterial; D012395:Rose Bengal