TRARESA: a tissue microarray-based hospital system for biomarker validation and discovery.
Formalin fixed and paraffin embedded tissue (FFPE) collections in pathology departments are the largest resource for retrospective biomedical research studies. Based on the literature analysis of FFPE related research, as well as our own technical validation, we present the Translational Research Arrays (TRARESA), a tissue microarray centred, hospital based, translational research conceptual framework for both validation and/or discovery of novel biomarkers. TRARESA incorporates the analysis of protein, DNA and RNA in the same samples, correlating with clinical and pathological parameters from each case, and allowing (a) the confirmation of new biomarkers, disease hypotheses and drug targets, and (b) the postulation of novel hypotheses on disease mechanisms and drug targets based on known biomarkers. While presenting TRARESA, we illustrate the use of such a comprehensive approach. The conceptualisation of the role of FFPE-based studies in translational research allows the utilisation of this commodity, and adds to the hypothesis-generating armamentarium of existing high-throughput technologies.
Das
Kakoli
K
Department of Pathology, National University Hospital, Singapore.
Mohd Omar
Mohd Feroz
MF
Ong
Chee Wee
CW
Bin Abdul Rashid
Suhaimi
S
Peh
Bee Keow
BK
Putti
Thomas Choudary
TC
Tan
Puay Hoon
PH
Chia
Kee Seng
KS
Teh
Ming
M
Shah
Nilesh
N
Soong
Richie
R
Salto-Tellez
Manuel
M
D015415:Biomarkers / Q000032:analysis*; D004247:DNA / Q000032:analysis; D005557:Formaldehyde; D006761:Hospitals; D006801:Humans; D016612:Paraffin Embedding; D012313:RNA / Q000032:analysis; D046888:Tissue Array Analysis / Q000379:methods*; D016707:Tissue Fixation; D054928:Validation Studies as Topic
D015415:Biomarkers; D005557:Formaldehyde; D012313:RNA; D004247:DNA