Rapid culture-free identification and molecular typing of Shiga toxin-producing Escherichia coli by PCR-RFLP.
Using culture-independent technology, PCR-RFLP were used to identify and type STEC in the stool of a patient with HUS. Fecal PCR-RFLP patterns were identical to those of the STEC O157:H7 isolated from the patient.
Shima
Kensuke
K
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Naka-ku, Sakai, Osaka, Japan.
Kawamura
Naohisa
N
Hinenoya
Atsushi
A
Sugimoto
Norihiko
N
Wu
Yuluo
Y
Asakura
Masahiro
M
Nishimura
Kazuhiko
K
Nair
Gopinath B
GB
Yamasaki
Shinji
S
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000235:genetics; D003937:Diagnosis, Differential; D003967:Diarrhea / Q000175:diagnosis* / Q000382:microbiology; D004927:Escherichia coli Infections / Q000175:diagnosis* / Q000382:microbiology; D019453:Escherichia coli O157 / Q000235:genetics / Q000302:isolation & purification*; D005240:Feasibility Studies; D005243:Feces / Q000382:microbiology*; D006463:Hemolytic-Uremic Syndrome / Q000175:diagnosis* / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D004269:DNA, Bacterial