title: Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice.
abstract: In the present work we analyze the antigenicity of Leishmania major ribosomal proteins (LRP) in infected BALB/c mice. We show that BALB/c mice vaccinated with LRP in the presence of CpG oligodeoxynucleotides (CpG-ODN) were protected against the development of dermal pathology and showed a reduction in the parasite load after challenge with L. major. This protection was associated with the induction of an IL-12 dependent specific-IFN-gamma response mediated mainly by CD4(+) T cell, albeit a minor contribution of CD8(+) T cells cannot be ruled out. Induction of Th1 responses against LRP also resulted in a reversion of the Th2 responses associated with susceptibility. A marked reduction of IgG1 antibody titer against parasite antigens besides an impaired IL-4 and IL-10 cytokine production by parasite specific T cells was observed. In addition, we show that the administration of the LRP plus CpG-ODN preparation also conferred protection in the naturally resistant C57BL/6 mice. In this strain protection was associated with a LRP specific IFN-gamma production in lymph nodes draining the challenge site. We believe that these evolutionary conserved proteins, combined with adjuvants that favor Th1 responses, may be relevant components of a pan-Leishmania vaccine.
authors.affiliation: Unidad de Inmunología Viral, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Crta. Pozuelo Km 2, 28220 Majadahonda, Madrid, Spain.
authors.forename: Salvador
authors.initials: S
authors.lastname: Iborra
authors.forename: Nuria
authors.initials: N
authors.lastname: Parody
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Abánades
authors.forename: Pedro
authors.initials: P
authors.lastname: Bonay
authors.forename: Deboraci
authors.initials: D
authors.lastname: Prates
authors.forename: Fernanda O
authors.initials: FO
authors.lastname: Novais
authors.forename: Manoel
authors.initials: M
authors.lastname: Barral-Netto
authors.forename: Carlos
authors.initials: C
authors.lastname: Alonso
authors.forename: Manuel
authors.initials: M
authors.lastname: Soto
mesh_terms: D000818:Animals; D005260:Female; D007074:Immunoglobulin G / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D018320:Leishmania major / Q000276:immunology*; D054332:Leishmaniasis Vaccines; D016773:Leishmaniasis, Cutaneous / Q000276:immunology / Q000517:prevention & control*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009838:Oligodeoxyribonucleotides / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: C408982:CPG-oligonucleotide; D007074:Immunoglobulin G; D054332:Leishmaniasis Vaccines; D009838:Oligodeoxyribonucleotides; D015800:Protozoan Proteins; D012269:Ribosomal Proteins; D016753:Interleukin-10; D015847:Interleukin-4; D007371:Interferon-gamma