Genome-wide identification of haploinsufficiency in fission yeast.
Abnormal phenotypes resulting from haploinsufficiency (HI) are due to the loss of one allele. Recent studies in budding yeast have shown that HI originates from insufficient protein levels or from a stoichiometric imbalance between subunits of protein complexes. In humans, however, HI often involves transcription factors. Therefore, the species differences in HI and the molecular mechanisms of species-specific HI remain under investigation. In this study, HI in fission yeast was systematically surveyed. HI in fission yeast affected genes related to signaling and to basic cellular processes, as observed in budding yeast. These results suggest that there are species differences in HI and that the HI that occurs in fission yeast is intermediate to and HI in budding yeast and humans.
Baek
Seung Tae
ST
Functional Genomics Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
Han
Sangjo
S
Nam
Miyoung
M
Kim
Young-Dae
YD
Kim
Lila
L
Lee
Hyun-Jee
HJ
Heo
Kyung-Sun
KS
Lee
Hyemi
H
Lee
Minho
M
Park
Song-Kyu
SK
Maeng
Pil Jae
PJ
Park
Youngwoo
Y
Lee
Sunghou
S
Kim
Dong-Uk
DU
Kim
Dongsup
D
Hoe
Kwang-Lae
KL
D000483:Alleles; D005656:Fungal Proteins / Q000235:genetics; D017353:Gene Deletion; D018628:Gene Dosage; D005800:Genes, Fungal; D016681:Genome, Fungal; D010641:Phenotype; D012568:Schizosaccharomyces / Q000235:genetics* / Q000254:growth & development; D013045:Species Specificity
D005656:Fungal Proteins