Growth kinetics of Listeria monocytogenes in broth and beef frankfurters--determination of lag phase duration and exponential growth rate under isothermal conditions.
The objective of this study was to develop a new kinetic model to describe the isothermal growth of microorganisms. The new model was tested with Listeria monocytogenes in tryptic soy broth and frankfurters, and compared with 2 commonly used models-Baranyi and modified Gompertz models. Bias factor (BF), accuracy factor (AF), and root mean square errors (RMSE) were used to evaluate the 3 models. Either in broth or in frankfurter samples, there were no significant differences in BF (approximately 1.0) and AF (1.02 to 1.04) among the 3 models. In broth, the mean RMSE of the new model was very close to that of the Baranyi model, but significantly lower than that of the modified Gompertz model. However, in frankfurters, there were no significant differences in the mean RMSE values among the 3 models. These results suggest that these models are equally capable of describing isothermal bacterial growth curves. Almost identical to the Baranyi model in the exponential and stationary phases, the new model has a more identifiable lag phase and also suggests that the bacteria population would increase exponentially until the population approaches to within 1 to 2 logs from the stationary phase. In general, there is no significant difference in the means of the lag phase duration and specific growth rate between the new and Baranyi models, but both are significantly lower than those determined from the modified Gompertz models. The model developed in this study is directly derived from the isothermal growth characteristics and is more accurate in describing the kinetics of bacterial growth in foods.
Huang
L
L
U.S. Dept. of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. lihan.huang@ars.usda.gov
D000818:Animals; D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D005163:Factor Analysis, Statistical; D005516:Food Microbiology; D006801:Humans; D007700:Kinetics; D008089:Listeria monocytogenes / Q000254:growth & development*; D008461:Meat Products / Q000382:microbiology*; D008954:Models, Biological; D013696:Temperature; D013997:Time Factors
D003470:Culture Media