Calcineurin localizes to the hyphal septum in Aspergillus fumigatus: implications for septum formation and conidiophore development.
A functional calcineurin A fusion to enhanced green fluorescent protein (EGFP), CnaA-EGFP, was expressed in the Aspergillus fumigatus DeltacnaA mutant. CnaA-EGFP localized in actively growing hyphal tips, at the septa, and at junctions between the vesicle and phialides in an actin-dependent manner. This is the first study to implicate calcineurin in septum formation and conidiophore development of a filamentous fungus.
Juvvadi
Praveen Rao
PR
Department of Pediatrics, Duke University Medical Center, Durham, NC 27710, USA.
Fortwendel
Jarrod R
JR
Pinchai
Nadthanan
N
Perfect
B Zachary
BZ
Heitman
Joseph
J
Steinbach
William J
WJ
D001232:Aspergillus fumigatus / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development*; D019703:Calcineurin / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D025301:Hyphae / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D021381:Protein Transport; D013172:Spores, Fungal / Q000201:enzymology / Q000235:genetics / Q000254:growth & development*
D005656:Fungal Proteins; D019703:Calcineurin