title: Immunohistochemistry using antibodies to the cathelicidin LL37/hCAP18 in the tammar wallaby, Macropus eugenii.
abstract: Antibodies to the human cathelicidin, hCAP18 have been used to examine epithelial tissues of adult and pouch young marsupials. Immunoreactivity was observed in skin, gastrointestinal tract, lung and mammary node of adults as well as skin, gastrointestinal tract, lung and bone marrow of pouch young. The locations of expression were similar to that reported in human tissues. Although the antibody to hCAP18 is primarily directed at the C-terminal antimicrobial peptide LL37, our observations suggest recognition of a common conserved element of this cathelicidin and lead us to conclude that the epithelial tissues of marsupials are sites of production of cathelicidin. This is consistent with observations in other mammals but is the first report of expression of these compounds in marsupials.
authors.affiliation: Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, NSW, Australia.
authors.forename: Rebecca L
authors.initials: RL
authors.lastname: Carman
authors.forename: Mary R
authors.initials: MR
authors.lastname: Simonian
authors.forename: Julie M
authors.initials: JM
authors.lastname: Old
authors.forename: Nicholas A
authors.initials: NA
authors.lastname: Jacques
authors.forename: Elizabeth M
authors.initials: EM
authors.lastname: Deane
mesh_terms: D000367:Age Factors; D000595:Amino Acid Sequence; D000818:Animals; D000906:Antibodies; D000918:Antibody Specificity; D023181:Antimicrobial Cationic Peptides / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D004848:Epithelium / Q000276:immunology / Q000378:metabolism; D000939:Epitopes / Q000235:genetics / Q000276:immunology; D005260:Female; D006801:Humans; D007107:Immune System / Q000502:physiology; D007150:Immunohistochemistry; D007614:Macropodidae / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D008969:Molecular Sequence Data; D013045:Species Specificity; D054804:Cathelicidins
chemical_list: D000906:Antibodies; D023181:Antimicrobial Cationic Peptides; D000939:Epitopes; D054804:Cathelicidins