Transient response method for evaluating the rate constants of reactions over immobilized enzymes.
The transient response method was utilized to evaluate the rate constants of reaction over immobilized enzyme. Glucose oxidation catalyzed by the immobilized glucose oxidase in a fixed-bed reactor was selected as an example. A theoretical model including the effects of axial dispersion, film diffusion, and intraparticle diffusion was established for the reactor. The individual rate constant of each elementary step of this enzymatic reaction was determined through direct fitting of the experimental response data to the model.
Wu
J Y
JY
Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China.
Weng
H S
HS