title: Oxidative injury in the cerebral cortex and subplate neurons in periventricular leukomalacia.
abstract: We previously identified immunocytochemical evidence of nitrative and oxidative injury in premyelinating oligodendrocytes in periventricular leukomalacia (PVL). Here, we tested the hypothesis that free radical injury occurs in the overlying cerebral cortex and subplate neurons in PVL. We immunostained for nitrotyrosine, malondialdehyde, and hydroxynonenal adducts and scored neuron staining density in PVL (n = 11) and non-PVL (n = 15) cases (postconceptional ages from 34 to 109 weeks). Analysis of covariance controlled for age. Mean malondialdehyde scores in PVL cases were increased over controls (p = 0.005). Hydroxynonenal scores increased with age only in PVL cases (diagnosis vs age interaction; p = 0.024). Nitrotyrosine scores were not significantly increased. In 11 PVL and 23 control cases between 20 and 183 postconceptional weeks, cells morphologically consistent with subplate and Cajal-Retzius neurons showed qualitatively increased free radical modification in PVL over control cases with statistically significant odds ratios for hydroxynonenal and nitrotyrosine in both subplate neurons and Cajal-Retzius cells. Glial fibrillary acidic protein and CD68 scores for reactive astrocytes and microglia, respectively, were not significantly increased, suggesting a minimal inflammatory response. Thus, oxidative/nitrative damage to cortical and "pioneer" neurons, although mild overall, may contribute to cortical volume loss and cognitive/behavioral impairment in survivors of prematurity.
authors.affiliation: Department of Pathology, Children's Hospital, Boston, MA, USA. rfolkerth@partners.org
authors.forename: Rebecca D
authors.initials: RD
authors.lastname: Folkerth
authors.forename: Felicia L
authors.initials: FL
authors.lastname: Trachtenberg
authors.forename: Robin L
authors.initials: RL
authors.lastname: Haynes
mesh_terms: D000447:Aldehydes / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001253:Astrocytes / Q000378:metabolism / Q000473:pathology; D001930:Brain Injuries / Q000150:complications / Q000473:pathology*; D002452:Cell Count / Q000379:methods; D002540:Cerebral Cortex / Q000293:injuries / Q000473:pathology*; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007969:Leukomalacia, Periventricular / Q000150:complications / Q000473:pathology*; D016015:Logistic Models; D008297:Male; D009474:Neurons / Q000473:pathology*; D010084:Oxidation-Reduction; D000097382:CD68 Molecule
chemical_list: D000447:Aldehydes; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D005904:Glial Fibrillary Acidic Protein; C067980:CD68 antigen, human; D000097382:CD68 Molecule; C027576:4-hydroxy-2-nonenal