title: Tastes and structures of bitter peptide, asparagine-alanine-leucine-proline-glutamate, and its synthetic analogues.
abstract: Asn-Ala-Leu-Pro-Glu (NALPE) is a strong bitter peptide with a minimum response threshold (MRT) of 0.074 mM. To elucidate the relationship of spatial structure and bitterness on peptides, NALPE and its analogues, NALPW, NALPS, NALPL, NALPP, NALPD, and NALPR, were synthesized and sensorially evaluated. Structural analysis using computer simulation for each peptide revealed that the presence of a polar group and hydrophobic bitter amino acids, the composition of hydrophobic regions, the spatial orientation of the polar group and hydrophobic regions, and the proximity between polar groups and hydrophobic regions faced within the same plane space may be the major determinants for the taste type and intensity of peptide bitterness.
authors.affiliation: School of Life Science and Biotechnology, Korea University, Seoul 136-701, Korea [corrected]
authors.forename: Mi-Ryung
authors.initials: MR
authors.lastname: Kim
authors.forename: Kawamura
authors.initials: K
authors.lastname: Yukio
authors.forename: Ki Myong
authors.initials: KM
authors.lastname: Kim
authors.forename: Cherl-Ho
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D003198:Computer Simulation; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D013649:Taste
chemical_list: D009842:Oligopeptides; C531027:asparagyl-alanyl-leucyl-phenylalanyl-glutamic acid