[Analysis of rice OsPLD3 and OsPLD4 genes and promoters].
Phospholipase Ds (PLDs) exist in many plants. PLDs catalyse the hydrolysis of phospholipids (e.g. phosphatidylcholine) in cell membrane into phosphatidic acid (PA) and polar free heads (e.g. choline). Two PLD members from rice, OsPLD3 and OsPLD4, were studied by reverse genetics approaches. The results showed that the promoters of OsPLD3 and OsPLD4 could drive the expression of the reporter gene in various tissues of the rice flower organs at different levels. The expression of both genes was induced by wounding and methyl jasmonate (MeJA), but with different intensity at different time intervals. No prominent phenotypes were observed by RNA interference with the gene-specific artificial miRNAs or over-expression of the target genes in rice plants, implying the functional redundancy among different members of the rice PLD family.
Yang
Kun
K
State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Fu
Yaping
Y
Zhang
Yuman
Y
Yan
Yongsheng
Y
Zhao
Zhiqiang
Z
Fang
Rongxiang
R
Sun
Zongxiu
Z
Chen
Xiaoying
X
D000085:Acetates / Q000494:pharmacology; D001483:Base Sequence; D003517:Cyclopentanes / Q000494:pharmacology; D035264:Flowers / Q000235:genetics / Q000378:metabolism; D017343:Genes, Plant / Q000235:genetics*; D017930:Genes, Reporter / Q000235:genetics; D008969:Molecular Sequence Data; D012275:Oryza / Q000201:enzymology / Q000235:genetics*; D054883:Oxylipins / Q000494:pharmacology; D010739:Phospholipase D / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics*
D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; C072239:methyl jasmonate; D010739:Phospholipase D