title: Enantiomeric purity of biodegradation products of juvenogens by newly isolated soil bacteria.
abstract: Two bacteria were isolated from sand RQ30, characterized as Bacillus simplex and Bacillus sp. strain 05 (GenBank EU399813 ), and were used as biocatalysts for a hydrolytic assay of stability of the cis or trans isomers of ethyl N-{2-{4-{[2-(butanoyl)oxycyclohexyl]methyl}phenoxy}ethyl}carbamate, which are among insect hormonogen substances (juvenogens). The stability tests were performed using simple modeling under laboratory conditions. The structures of the products were assigned as ethyl (1 R,2 R)- N-{2-{4-[(2-hydroxycyclohexyl)methyl]phenoxy}ethyl}carbamate and ethyl (1 S,2 R)- N-{2-{4-[(2-hydroxycyclohexyl)methyl]phenoxy}ethyl}carbamate on the basis of (1)H and (13)C NMR, IR, and FAB-MS analyses.
authors.affiliation: Institute of Organic Chemistry and Biochemistry AS CR, Flemingovo namesti 2, 166 10 Prague 6, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Novák
authors.forename: Richard
authors.initials: R
authors.lastname: Tykva
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Wimmer
authors.forename: Milan
authors.initials: M
authors.lastname: Pavlík
authors.forename: Marek
authors.initials: M
authors.lastname: Prouza
authors.forename: Klára
authors.initials: K
authors.lastname: Hlavsová
authors.forename: Marie
authors.initials: M
authors.lastname: Zarevúcka
mesh_terms: D001407:Bacillus / Q000378:metabolism*; D002219:Carbamates / Q000737:chemistry / Q000378:metabolism; D003511:Cyclohexanols / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D007605:Juvenile Hormones / Q000737:chemistry* / Q000378:metabolism*; D012988:Soil Microbiology; D013237:Stereoisomerism
chemical_list: D002219:Carbamates; D003511:Cyclohexanols; D007605:Juvenile Hormones