Crystallization and preliminary X-ray diffraction analysis [correction of anaylsis] of the LOV1 domains of phototropin 1 and 2 from Arabidopsis thaliana.
Phototropin is a blue-light receptor protein in plants that is responsible for phototropic responses, stomata opening and photo-induced relocation of chloroplasts. Higher plants such as Arabidopsis thaliana have two isoforms of phototropin: phototropin 1 and phototropin 2. Both isoforms comprise a tandem pair of blue-light-absorbing light-oxygen-voltage domains named LOV1 and LOV2 in the N-terminal half and a serine/threonine kinase domain in the C-terminal half. The LOV1 domain is thought to function as a dimerization site. In the present study, recombinant LOV1 domains of A. thaliana phototropin 1 and phototropin 2 were crystallized. The crystal of the LOV1 domain of phototropin 1 belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 61.2, b = 64.9, c = 70.8 A, and diffracted X-rays to a resolution of 2.1 A. The crystal of the LOV1 domain of phototropin 2 belonged to space group P2(1), with unit-cell parameters a = 32.5, b = 66.5, c = 56.7 A, beta = 92.4 degrees , and diffracted X-rays to beyond 2.0 A resolution. In both crystals, two LOV1 domains occupied the crystallographic asymmetric unit.
Nakasako
Masayoshi
M
Department of Physics, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Kanagawa 223-8522, Japan. nakasako@phys.keio.ac.jp
Hirata
Michihiro
M
Shimizu
Nobutaka
N
Hosokawa
Shyuntaro
S
Matsuoka
Daisuke
D
Oka
Toshihiko
T
Yamamoto
Masaki
M
Tokutomi
Satoru
S
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000737:chemistry*; D029681:Arabidopsis Proteins / Q000737:chemistry* / Q000235:genetics; D056931:Cryptochromes; D003460:Crystallization; D004268:DNA-Binding Proteins / Q000737:chemistry* / Q000235:genetics; D005420:Flavoproteins / Q000737:chemistry* / Q000235:genetics; D008969:Molecular Sequence Data; D010750:Phosphoproteins / Q000737:chemistry* / Q000235:genetics; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics; D017346:Protein Serine-Threonine Kinases; D017434:Protein Structure, Tertiary; D014961:X-Ray Diffraction
D029681:Arabidopsis Proteins; C538962:CRY1 protein, Arabidopsis; D056931:Cryptochromes; D004268:DNA-Binding Proteins; D005420:Flavoproteins; C450148:LKP2 protein, Arabidopsis; C527342:LOV1 protein, Arabidopsis; C423903:PHOT2 protein, Arabidopsis; D010750:Phosphoproteins; D020033:Protein Isoforms; C103646:NPH1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases