[Inhibitory effects of sinapine on activity of acetylcholinesterase in cerebral homogenate and blood serum of rats].
OBJECTIVE
The present study investigated the inhibitory effects of Chinese herb component sinapine on activity of acetylcholinesterase (AChE) in cerebral homogenate and blood serum of rats.


METHODS
AChE was prepared from cerebral homogenate and blood serum of rats, respectively. Acetylcholinesterase activity assay kit and Chromatometry were used to detect the AChE activity.


RESULTS
Sinapine significantly inhibited AChE activity in vitro, with more effective on cerebral homogenate (IC50 3.66 micromol x L(-1)) than blood serum (IC50 22.1 micromol x L(-1)).


CONCLUSIONS
Sinapine could significantly inhibit the cerebral AChE activity and may be a promising drug used for prevention and cure of Alzheimer's disease as a cholinesterase inhibitor.
He
Ling
L
Postdoctoral scientific research afflux station, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
Li
Hai-Tao
HT
Guo
Sheng-Wei
SW
Liu
Li-Fang
LF
Qiu
Jia-Bin
JB
Li
Fu
F
Cai
Bao-Chang
BC
D000110:Acetylcholinesterase / Q000097:blood* / Q000378:metabolism*; D000544:Alzheimer Disease / Q000188:drug therapy / Q000517:prevention & control; D000818:Animals; D001921:Brain / Q000166:cytology / Q000201:enzymology*; D002794:Choline / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D002800:Cholinesterase Inhibitors / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats
D002800:Cholinesterase Inhibitors; C005685:sinapine; D000110:Acetylcholinesterase; D002794:Choline