title: Detection and relative quantification of proteins by surface enhanced Raman using isotopic labels.
abstract: Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix format without interference from protein features.
authors.affiliation: Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: Shirshendu K
authors.initials: SK
authors.lastname: Deb
authors.forename: Brandon
authors.initials: B
authors.lastname: Davis
authors.forename: Giselle M
authors.initials: GM
authors.lastname: Knudsen
authors.forename: Ravindra
authors.initials: R
authors.lastname: Gudihal
authors.forename: Dor
authors.initials: D
authors.lastname: Ben-Amotz
authors.forename: V Jo
authors.initials: VJ
authors.lastname: Davisson
mesh_terms: D005456:Fluorescent Dyes / Q000737:chemistry*; D007553:Isotope Labeling; D011506:Proteins / Q000032:analysis*; D012235:Rhodamines / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes; D011506:Proteins; D012235:Rhodamines; C026188:rhodamine 6G