title: Semicontinuous cultivation of Digitalis lanata cells: production of beta-methyldigoxin in a 300-L airlift bioreactor.
abstract: Selected Digitalis lanata cell lines cultivated in 1-L shake flasks or 20-L airlift bioreactors converted beta-methyldigitoxin into beta-methyldigoxin with almost no side reactions. This biotransformation process was optimized with regard to substrate supply and culture medium composition, and was then scaled up to a volume of 210 L using a 300-L airlift bioreactor. A semicontinuous process was developed in which 513.3 g beta-methyldigoxin were produced after 89 days of cultivation.
authors.affiliation: Pharmaceutical Institute, University of Tuebingen, D-7400 Tuebingen, Federal Republic of Germany.
authors.forename: E
authors.initials: E
authors.lastname: Reinhard
authors.forename: W
authors.initials: W
authors.lastname: Kreis
authors.forename: U
authors.initials: U
authors.lastname: Barthlen
authors.forename: U
authors.initials: U
authors.lastname: Helmbold