title: Protein release from galactoglucomannan hydrogels: influence of substitutions and enzymatic hydrolysis by beta-mannanase.
abstract: O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95% released BSA after eight hours compared to 60% without enzyme. Thus, both the pendant group composition and the enzyme action are valuable tools in the tailoring of hydrogel release profiles of potential interest for intestine drug delivery.
authors.affiliation: Department of Biochemistry, Lund University, Post Office Box 124, SE-221 00 Lund, Sweden.
authors.forename: Alexandra Andersson
authors.initials: AA
authors.lastname: Roos
authors.forename: Ulrica
authors.initials: U
authors.lastname: Edlund
authors.forename: John
authors.initials: J
authors.lastname: Sjöberg
authors.forename: Ann-Christine
authors.initials: AC
authors.lastname: Albertsson
authors.forename: Henrik
authors.initials: H
authors.lastname: Stålbrand
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002241:Carbohydrates / Q000737:chemistry; D002417:Cattle; D016503:Drug Delivery Systems; D020100:Hydrogels / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry*; D008422:Materials Testing; D008689:Methacrylates / Q000737:chemistry; D008956:Models, Chemical; D012710:Serum Albumin, Bovine / Q000737:chemistry; D000519:alpha-Galactosidase / Q000737:chemistry; D044902:beta-Mannosidase / Q000737:chemistry*
chemical_list: D001672:Biocompatible Materials; D002241:Carbohydrates; D020100:Hydrogels; D008351:Mannans; D008689:Methacrylates; C443804:galactoglucomannan; D012710:Serum Albumin, Bovine; C005044:hydroxyethyl methacrylate; D000519:alpha-Galactosidase; D044902:beta-Mannosidase