title: Glutamatergic inputs to midbrain dopaminergic neurons in primates.
abstract: Anterograde tract-tracing and immunohistochemical methods were used to study projections from the pedunculopontine tegmental nucleus (PPN) to midbrain dopaminergic neurons in the squirrel monkey (Saimiri sciureus). The PPN harbored numerous cholinergic and glutamatergic neurons, as well as neurons that displayed both cholinergic and glutamatergic markers. Injections of anterograde tracer into the PPN led to intense fiber labeling in the substantia nigra pars compacta (SNc) and the ventral tegmental area (VTA). This pedunculonigral projection was partly bilateral. At the electron microscopic level, about 40-60% of the anterogradely labeled terminal boutons were glutamate-positive and formed asymmetric synapses with the dopaminergic neurons of the SNc-VTA complex. These data provide direct evidence for a pedunculonigral glutamatergic projection. This projection may play a crucial role in the control of the firing pattern of SNc-VTA dopaminergic neurons and could be involved in glutamate-mediated excitotoxicity that is believed to lead to SNc cell death in Parkinson's disease.
authors.affiliation: Laboratoire de Neurobiologie, Centre de recherche Université Laval Robert-Giffard, 2601, de la Canardiére, Beauport, Que., Canada G1J 2G3.
authors.forename: A
authors.initials: A
authors.lastname: Parent
authors.forename: M
authors.initials: M
authors.lastname: Parent
authors.forename: A
authors.initials: A
authors.lastname: Charara