title: Complete genome analysis of a highly pathogenic H5N1 influenza A virus isolated from a tiger in China.
abstract: An influenza A virus (A/Tig/SH/01/2005 (H5N1) was isolated from lung tissue samples of a dead zoo tiger with respiratory disease in China in July 2005. Complete genome analysis indicated that the isolate was highly identical to an H5N1 virus isolated from a migratory duck at Poyang lake in China in that year. The genotype of the isolate was K,G,D,5J,F,1J,F,1E, and phylogenetically it was a clade 2.2 virus. Molecular characterization of all of the gene segments revealed characteristics of highly pathogenic influenza A viruses. These results may help to identify molecular determinants of virulence and highlight the necessity for continuous surveillance.
authors.affiliation: Key Laboratory of Animal Diseases Diagnostic and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Ministry of Agriculture, Nanjing, China.
authors.forename: Muhammad Hassan
authors.initials: MH
authors.lastname: Mushtaq
authors.forename: Huang
authors.initials: H
authors.lastname: Juan
authors.forename: Ping
authors.initials: P
authors.lastname: Jiang
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.forename: TianXian
authors.initials: T
authors.lastname: Li
authors.forename: Yijun
authors.initials: Y
authors.lastname: Du
authors.forename: Muhammad Mahmood
authors.initials: MM
authors.lastname: Mukhtar
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D004372:Ducks / Q000821:virology; D005814:Genes, Viral; D016679:Genome, Viral; D005838:Genotype; D053124:Influenza A Virus, H5N1 Subtype / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification*; D008969:Molecular Sequence Data; D009976:Orthomyxoviridae Infections / Q000662:veterinary* / Q000821:virology*; D046010:Tigers / Q000821:virology*; D014774:Virulence / Q000235:genetics