title: [Determination of 5-hydroxymethyl furfuraldehyde in different processed products of polygonatum].
abstract: OBJECTIVE
  By finding the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Polygonatum for different time, this provides basis for the technology of processing Polygonatum and quality standards of its prepared pieces.
  
  
  METHODS
  The testing solution was acquired by ultrasonic extraction, HPLC was used to determine the contents. The chromatographic conditions: Agilent HC-C18 column (5 microm, 250 mm x 4.6 mm). The mobile phase: methanol-water (15:85) at the flow rate of 1 ml/min. The determination wave length was 284 nm.
  
  
  RESULTS
  All kinds of processed Polygonatum had 5-Hydroxymethyl furfuraldethyde in different degrees, contents in Polygonatum kept relatively stable before 30 hours, but contents rose rapidly after 30 hours, contents decreased as continuing heating.
  
  
  CONCLUSIONS
  The result can provide useful references to quality control and standard porcessing condition of Polygonatum.
authors.affiliation: Henan College of Traditional Chinese Medicine, Zhengzhou, China. yyun@china.com.cn
authors.forename: Yun
authors.initials: Y
authors.lastname: Yang
authors.forename: Min
authors.initials: M
authors.lastname: Xu
authors.forename: Yun-xia
authors.initials: YX
authors.lastname: Feng
authors.forename: Hai-hua
authors.initials: HH
authors.lastname: He
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000592:standards; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000032:analysis; D006358:Hot Temperature; D010946:Plants, Medicinal / Q000737:chemistry*; D031384:Polygonatum / Q000737:chemistry*; D011786:Quality Control; D012015:Reference Standards; D015203:Reproducibility of Results; D027343:Rhizome / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013997:Time Factors; D014465:Ultrasonics
chemical_list: D004365:Drugs, Chinese Herbal; C008046:5-hydroxymethylfurfural; D005662:Furaldehyde