title: Regulation of peripheral spindle movement and spindle rotation during mouse oocyte meiosis: new perspectives.
abstract: Spindle movement, including spindle migration during first meiosis and spindle rotation during second meiosis, is essential for asymmetric divisions in mouse oocytes. Previous studies by others and us have shown that microfilaments are required for both spindle migration and rotation. In the present study, we aimed to further investigate the mechanism controlling spindle movement during mouse oocyte meiosis. By employing drug treatment and immunofluorescence microscopy, we showed that dynamic microtubule assembly was involved in both spindle migration and rotation. Furthermore, we found that the calcium/CaM/CaMKII pathway was important for regulating spindle rotation.
authors.affiliation: State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jun-Shu
authors.initials: JS
authors.lastname: Ai
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wang
authors.forename: Shen
authors.initials: S
authors.lastname: Yin
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Shi
authors.forename: Bo
authors.initials: B
authors.lastname: Xiong
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: OuYang
authors.forename: Yi
authors.initials: Y
authors.lastname: Hou
authors.forename: Da-Yuan
authors.initials: DY
authors.lastname: Chen
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D008540:Meiosis; D051379:Mice; D008856:Microscopy, Fluorescence; D008870:Microtubules / Q000378:metabolism; D009068:Movement; D009865:Oocytes / Q000502:physiology*; D012399:Rotation; D008941:Spindle Apparatus
chemical_list: D002118:Calcium