[Inhibiting effects of TFPI-2 recombinant adenovirus on invasion of laryngeal squamous carcinoma].
OBJECTIVE
To study the effects of adenovirus-mediated tissue factor pathway inhibitor-2 gene on the invasion of laryngeal squamous cancer.


METHODS
Ad-TFPI-2 was transfected into laryngeal squamous cancer (Hep-2) cell. Western-blot was used to test the TFPI-2 protein expression and Boyden Chamber experiment was used to examine the invasive ability of Hep-2 cells. Furthermore, the Ad-TFPI-2 infected Hep-2 cells were subcutaneously inoculated in nude mice and the tumor formation capability were observed.


RESULTS
Ad-TFPI-2 was identified correctly by endonuclease and sequencing and the virus titer was 2.8 x 10(13) PFU/L. In the Hep-2 cells of treated group, the TFPI-2 protein expression was increased while the invasive capability was descent. The tumor formation capability was also decreased in the treated group nude mouse model.


CONCLUSIONS
TFPI-2 recombinant adenovirus can effectively inhibit the invasive capability of laryngeal squamous cancer.
Sun
Ya'nan
Y
Department of Otorhinolaryngology-Head and Neck Surgery, the Second Affiliated Hospital, Harbin Medical University, Harbin, 150086, China.
Liu
Ming
M
Xiao
Yuli
Y
Tian
Linli
L
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D015316:Genetic Therapy; D005822:Genetic Vectors; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D014162:Transfection
D006023:Glycoproteins; C021240:tissue-factor-pathway inhibitor 2