title: A high-resolution radiation hybrid map of rhesus macaque chromosome 5 identifies rearrangements in the genome assembly.
abstract: A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.
authors.affiliation: Department of Population Health and Reproduction, School of Veterinary Medicine, California National Primate Research Center, Davis, CA 95616, USA.
authors.forename: Genesio M
authors.initials: GM
authors.lastname: Karere
authors.forename: Lutz
authors.initials: L
authors.lastname: Froenicke
authors.forename: Lee
authors.initials: L
authors.lastname: Millon
authors.forename: James E
authors.initials: JE
authors.lastname: Womack
authors.forename: Leslie A
authors.initials: LA
authors.lastname: Lyons
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D002869:Chromosome Aberrations / Q000528:radiation effects*; D002874:Chromosome Mapping / Q000379:methods*; D033481:Chromosomes, Mammalian / Q000235:genetics*; D006801:Humans; D008253:Macaca mulatta; D008297:Male; D011839:Radiation, Ionizing