Identification of galectin-3 and mucin-type O-glycans in breast cancer and its metastasis to brain.
Galectin-3 has been implicated in tumor progression. We demonstrated immunohistochemically that galectin-3 was negative in normal breast tissue, but it was highly increased in breast cancer and in metastatic tissues to brain. Similarly, histochemistry with mucin-specific lectins showed increased recognition in breast tumor and metastasis with Machaerocereus eruca agglutinin (Fualpha 1,2 (GalNAcalpha 1,3) Galss1,4 in complex mucin) but not for Amaranthus leucocarpus (Galss1,3-GalNAc-alpha 1,0-Ser/Thr) and Arachis hypogaea lectins (Galss1,3GalNAc/Galss1,4GlcNAc). Mucin-type glycans and galectin-3 colocalized in breast cancer and metastasis, but not in normal tissue, suggesting upregulated biosynthesis of complex O-glycosidically linked glycans and galectin-3 favor breast cancer progression and brain metastasis.
Mayoral
M A
MA
Laboratorio Experimental de Enfermedades Neurodegenerativas, Instituto Nacional de Neurologia y Neurocirugia, Del Tlalpan, Mexico.
Mayoral
C
C
Meneses
A
A
Villalvazo
L
L
Guzman
A
A
Espinosa
B
B
Ochoa
J L
JL
Zenteno
E
E
Guevara
J
J
D010367:Arachis; D001932:Brain Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000556:secondary; D001943:Breast Neoplasms / Q000737:chemistry* / Q000378:metabolism / Q000473:pathology; D005260:Female; D037502:Galectin 3 / Q000032:analysis*; D006023:Glycoproteins; D006031:Glycosylation; D006651:Histocytochemistry / Q000379:methods; D006801:Humans; D007150:Immunohistochemistry; D009077:Mucins / Q000032:analysis* / Q000378:metabolism; D009439:Neuraminidase / Q000378:metabolism; D037121:Plant Lectins; D013048:Specimen Handling / Q000379:methods; D014357:Trypsin / Q000378:metabolism; D015854:Up-Regulation
C121359:Amaranthus leucocarpus lectin; D037502:Galectin 3; D006023:Glycoproteins; C057322:Machaerocereus eruca lectin; D009077:Mucins; D037121:Plant Lectins; D009439:Neuraminidase; D014357:Trypsin