title: [Bushen Jianpi Huoxue Recipe in prevention and treatment of tibial stress injuries in rabbits].
abstract: OBJECTIVE
  To explore the effects of Bushen Jianpi Huoxue Recipe, a compound traditional Chinese herbal medicine for tonifying the kidney, invigorating the spleen and promoting blood circulation, on tibial stress injuries in rabbits.
  
  
  METHODS
  Thirty-five mature male rabbits were used in the experiment, and randomly divided into 7 groups: sedentary control (SC) group, 1-week exercise (E-1W) group, 2-week exercise (E-2W) group, 3-week exercise (E-3W) group, 4-week exercise (E-4W) group, 3-week exercise and 1-week Chinese herbal medicine treatment (EMT-1W) group, and 4-week exercise and 2-week Chinese herbal medicine treatment (EMT-2W) group. There were 5 rabbits in each group. A rabbit model of tibial stress injuries was established by stimulating the rabbits to jump and run within a high-voltage and low-current electronic cage. Radiologic features, bone scintigraphy, histology and electron microscopy of rabbit tibia were observed, and the content of blood parathyroid hormone (PTH), bone gla protein (BGP) and testosterone was detected by using radioimmunoassay method.
  
  
  RESULTS
  After 2-week exercise, changes in histology and osteocytes had a tendency towards stress injuries. Serum PTH and BGP levels were remarkably increased, while serum testosterone level was lower than that in the SC group. Three- and four-week continuous exercise resulted in tibial stress injuries, and the positive changes were observed in X-ray features and radionuclide images. Compared with E-3W group, the levels of serum BGP and testosterone were decreased remarkably. A series of positive results such as prevailing negativeness of X-ray features and radionuclide images, increasing process of osteogenesis, typical osteogenic phase of osteocytes and favorable transformation of biochemical markers was shown in EMT-1W and EMT-2W groups. It also showed remarkable rising levels of serum BGP and testosterone and remarkable reducing level of serum PTH in EMT-1W and EMT-2W groups as compared with E-1W and E-2W groups.
  
  
  CONCLUSIONS
  Bushen Jianpi Huoxue Recipe can prevent and treat exercise-induced tibial stress injuries by stimulating new bone formation and increasing serum testosterone level.
authors.affiliation: Research Unit of Health Rehabilitation, Department of Basic Theory, Capital College of Physical Education, Beijing 100088, China. chenyouxue@cipe.net.cn
authors.forename: You-xue
authors.initials: YX
authors.lastname: Chen
authors.forename: Guo-ping
authors.initials: GP
authors.lastname: Li
authors.forename: Hong-en
authors.initials: HE
authors.lastname: Yao
authors.forename: Su-fan
authors.initials: SF
authors.lastname: Li
authors.forename: Li-gong
authors.initials: LG
authors.lastname: Duan
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D015775:Fractures, Stress / Q000517:prevention & control*; D008297:Male; D015675:Osteocalcin / Q000097:blood; D010281:Parathyroid Hormone / Q000097:blood; D008517:Phytotherapy; D011817:Rabbits; D011859:Radiography; D011897:Random Allocation; D013739:Testosterone; D013977:Tibia / Q000000981:diagnostic imaging / Q000293:injuries*; D013978:Tibial Fractures / Q000517:prevention & control*
chemical_list: D004365:Drugs, Chinese Herbal; D010281:Parathyroid Hormone; D015675:Osteocalcin; D013739:Testosterone