[Effects of Chinese compound prescription on bone metabolism of weightlessness rats simulated by suspension].
OBJECTIVE
To study the effects of Chinese medicine compound on bone metabolism of weightlessness rats simulated by tail suspension.


METHODS
Fifty Wistar rats were divided into 5 groups randomly: control group, model group, and low does, medium dose and high does treated group. The experiment period lasted 21 days. After the Chinese compound prescription and distilled water were orally given to treated groups, and control and model group for 7 days, respectively, the tail suspension experiment was performed for treated and model group, meanwhile administration of Chinese compound prescription and distilled water still lasted until the end of the experiment. Blood serum was collected for determination of alkaline phosphatase (ALP), bone gla protein (BGP), tartrate-resistant acid phosphatase (TRAP), femoral bone HOP. The changes of bone mineral density (BMD) of femoral bone and lumbar vertebra were observe.


RESULTS
Compared with control group, the ALP level of model group was markedly decreased (P < 0.05), no change of BGP, TRAP was not observed, the BMD of femoral bone and lumbar vertebra were decreased remarkably (P < 0.05), Compared with model group, the change of ALP level of treated groups was not significant for all treated groups, the BGP level and BMD for medium dose group were increased (P < 0.05), the TRAP level for medium dose and high does groups was decreased (P < 0.05)


CONCLUSIONS
The Chinese compound prescription can improve the bone formation and prevent bone loss via inhibiting bone absorption and improving ossify, bone mineral deposition and mineralization as well as increasing BMD, which leads to prevention and treatment of bone loss.
Tong
Hai-Ying
HY
Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Hu
Su-Min
SM
Zhou
Peng
P
Fu
Qian
Q
Yang
Jia
J
Gao
Xue-Min
XM
Zhang
Jian-Jun
JJ
Zhong
Gan-Sheng
GS
D000135:Acid Phosphatase / Q000378:metabolism; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D015519:Bone Density / Q000187:drug effects; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism* / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019417:Hindlimb Suspension; D007527:Isoenzymes / Q000378:metabolism; D008297:Male; D015675:Osteocalcin / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D000071681:Tartrate-Resistant Acid Phosphatase; D018474:Weightlessness Simulation
D004365:Drugs, Chinese Herbal; D007527:Isoenzymes; D015675:Osteocalcin; D000469:Alkaline Phosphatase; D000135:Acid Phosphatase; D000071681:Tartrate-Resistant Acid Phosphatase