An integrated optofluidic platform for Raman-activated cell sorting.
We report on integrated optofluidic Raman-activated cell sorting (RACS) platforms that combine multichannel microfluidic devices and laser tweezers Raman spectroscopy (LTRS) for delivery, identification, and simultaneous sorting of individual cells. The system allows label-free cell identification based on Raman spectroscopy and automated continuous cell sorting. Two optofluidic designs using hydrodynamic focusing and pinch-flow fractionation are evaluated based on their sorting design and flow velocity effect on the laser trapping efficiency at different laser power levels. A proof-of-principle demonstration of the integrated optofluidic LTRS system for the identification and sorting of two leukemia cell lines is presented. This functional prototype lays the foundation for the development of a label-free cell sorting platform based on intrinsic Raman markers for automated sampling and sorting of a large number of individual cells in solution.
Lau
Adrian Y
AY
Applied Physics and Biophysics Division, Lawrence Livermore National Laboratory, P.O. Box 808, L-211, Livermore, CA 94550, USA.
Lee
Luke P
LP
Chan
James W
JW
D002469:Cell Separation / Q000295:instrumentation*; D008214:Lymphocytes / Q000166:cytology; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D055095:Optics and Photonics; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*