Mast cells play a critical role in the pathogenesis of viral myocarditis.
BACKGROUND
Mast cells are powerful producers of multiple cytokines and chemical mediators playing a pivotal role in the pathogenesis of various cardiovascular diseases. We examined the role of mast cells in murine models of heart failure due to viral myocarditis, using 2 strains of mast cell-deficient mice.


RESULTS
Two strains of mast cell-deficient mice, WBB6F1-Kit(W)/Kit(W-v) (W/W(V)) and WCB6F1-Kitl(Sl)/Kitl(Sl-d) (Sl/Sl(d)), were inoculated with 10 plaque-forming units of the encephalomyocarditis virus intraperitoneally. On day 14 after inoculation, survival of W/W(V) mice was significantly higher than that of their control littermates (77% versus 31%; P=0.03; n=13). On histological examination on day 7, myocardial necrosis and cellular infiltration were significantly less pronounced in W/W(V) and Sl/Sl(d) mice than in their control littermates (area of infiltration, 7.6+/-3.5% versus 29.3+/-15.6%; P=0.002; area of necrosis, 7.6+/-3.5% versus 30.0+/-17.2%; P=0.003; n=10). Histological examination showed more severe changes in mast cell-reconstituted than in -nonreconstituted W/W(V) and Sl/Sl(d) mice. The gene expressions of mast cell proteases were upregulated in the acute phase of viral myocarditis and rose further in the subacute phase of heart failure. Their activation coincided with the development of myocardial necrosis and fibrosis and correlated with the upregulation of gene expression of matrix metalloproteinase-9. The histamine H1-receptor antagonist bepotastine improved encephalomyocarditis viral myocarditis.


CONCLUSIONS
These observations suggest that mast cells participate in the acute inflammatory reaction and the onset of ventricular remodeling associated with acute viral myocarditis and that the inhibition of their function may be therapeutic in this disease.
Higuchi
Hirokazu
H
Department of Cardiovascular Medicine, Kyoto University Graduate School of Medicine, Japan.
Hara
Masatake
M
Yamamoto
Kanjo
K
Miyamoto
Tadashi
T
Kinoshita
Makoto
M
Yamada
Tasuku
T
Uchiyama
Koji
K
Matsumori
Akira
A
D004686:Encephalomyocarditis virus / Q000472:pathogenicity*; D005355:Fibrosis; D006333:Heart Failure; D007249:Inflammation / Q000209:etiology / Q000473:pathology; D008407:Mast Cells / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D009205:Myocarditis / Q000209:etiology / Q000821:virology*; D009336:Necrosis; D010447:Peptide Hydrolases / Q000235:genetics; D015996:Survival Rate; D015854:Up-Regulation / Q000235:genetics; D020257:Ventricular Remodeling / Q000235:genetics
D010447:Peptide Hydrolases; D020780:Matrix Metalloproteinase 9