Miniaturized multiphoton microscope with a 24Hz frame-rate.
With miniaturized tube lenses and a micro-electro-mechanical system (MEMS) mirror, we constructed a miniaturized multiphoton microscope system. Through a two-dimensional asynchronous scanning of the MEMS mirror, 24Hz frame rate can be realized. With a high numerical aperture objective, sub-micron resolution can also be achieved at the same time.
Liu
Tzu-Ming
TM
Department of Electrical Engineering and Graduate Institute of Photonics and Optoelectronics, National TaiwanUniversity, Taipei, ROC.
Chan
Ming-Che
MC
Chen
I-Hsiu
IH
Chia
Shih-Hsuan
SH
Sun
Chi-Kuang
CK
D000818:Animals; D002417:Cattle; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry; D007091:Image Processing, Computer-Assisted; D007368:Interferometry / Q000295:instrumentation; D007909:Lenses; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D008904:Miniaturization; D008962:Models, Theoretical; D055095:Optics and Photonics; D017785:Photons; D013710:Tendons / Q000473:pathology
D005456:Fluorescent Dyes