Cl-channel blockers inhibit cell proliferation and arrest the cell cycle of human ovarian cancer cells.
OBJECTIVE
To investigate the role of chloride channels in cell proliferation and cell cycles of human ovarian cancer cell line A2780.


METHODS
Chloride channel blockers were used to observe the effects of chloride channels on A2780 cells with MTT assay and flow cytometry.


RESULTS
NPPB (100 microM) significantly inhibited the cell proliferation and affected the cell cycle, which increased the percentage of cells in the G1 phase, and reduced it in the S phase. NFA (100 microM) and TAM (30 microM) had similar inhibitory effects. Glibenclamide (100 microM), however, had no effect on cell proliferation or cycle. Moreover, chloride channel blockers could inhibit Ca2+ influx in these cells.


CONCLUSIONS
Chloride channels, voltage-gated chloride channels, and volume-sensitive chloride channels especially, play an important role in the cell proliferation and cycle of A2780 cells. It is likely that the influence of chloride channels on cell proliferation and cell cycle is mediated by a Ca2+-dependent mechanism.
Li
M
M
Department of Obstetrics and Gynecology, Qilu Hospital, Shandong University, Jinan, China.
Wang
B
B
Lin
W
W
D002453:Cell Cycle / Q000187:drug effects / Q000502:physiology*; D045744:Cell Line, Tumor / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D018118:Chloride Channels / Q000037:antagonists & inhibitors / Q000502:physiology*; D005260:Female; D006801:Humans; D010051:Ovarian Neoplasms / Q000473:pathology*
D018118:Chloride Channels