Minor role of bystander tolerance to fetal calf serum in a peptide-specific dendritic cell vaccine model against autoimmunity: comparison with serum-free cultures.
Dendritic cells (DCs) are currently considered as promising tools for vaccination against tumors and also autoimmune responses. A major point of concern has been the use of fetal calf serum (FCS) as a source of heterologous antigen in DC cultures. FCS peptides can be presented by the DCs and cause T-cell responses in the recipient. We investigated the role of FCS in an autoimmune model where DC injections can prevent peptide-specifically from experimental autoimmune encephalomyelitis (EAE). We show that murine bone marrow-derived DCs generated in FCS-containing or serum-free media resulting in a similar phenotype, maturation potential, and functions. Peptide-specific protection could be achieved similarly with FCS-DC or serum-free DCs. Although FCS-DC induced strong CD4 T cell proliferation and cytokine production against FCS, these T cells lack antigenic recall during EAE. Even if FCS was reinjected, the effect on EAE resulted only in a 3-day delay of disease onset. Together, our data show that presentation of bystander antigens by peptide-specific DC vaccinations may have little influence on T-cell responses in vivo if the bystander antigen cannot be recalled by specific T cells.
Röner
Susanne
S
Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.
Zinser
Elisabeth
E
Menges
Mauritius
M
Wiethe
Carsten
C
Littmann
Leonie
L
Hänig
Jens
J
Steinkasserer
Alexander
A
Lutz
Manfred B
MB
D000818:Animals; D017951:Antigen Presentation / Q000276:immunology*; D001798:Blood Proteins / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002417:Cattle; D002454:Cell Differentiation / Q000276:immunology*; D049109:Cell Proliferation / Q000187:drug effects; D016895:Culture Media, Serum-Free; D003713:Dendritic Cells / Q000276:immunology*; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000276:immunology / Q000517:prevention & control*; D008213:Lymphocyte Activation / Q000276:immunology*; D051379:Mice; D010455:Peptides / Q000276:immunology; D037182:T-Cell Antigen Receptor Specificity / Q000187:drug effects / Q000276:immunology; D014611:Vaccination
D001798:Blood Proteins; D016895:Culture Media, Serum-Free; D010455:Peptides