Determination of pyruvic acid by using enzymic fluorescence capillary analysis.
A new method (P-LE-FCA) for the determination of pyruvic acid was proposed based on liquid enzyme method (LE) and fluorescence capillary analysis (FCA). The optimum experimental conditions were as follows: the excitation and emission wavelengths were 350 and 460 nm, respectively; the reaction time and temperature were 20 min and 38 degrees C, respectively; the pH of phosphate buffer solution was 7.5; the concentrations of nicotinamide adenine dinucleotide and lactate dehydrogenase were 1.0 mmol L(-1) and 5.0 k UL(-1), respectively. The linear range of this method was 0.2-1.2 mmol L(-1) (Delta F=327.13C-10.018, r=0.9942). Its detection limit was 0.012 mmol L(-1). And its relative standard deviation was 0.86%. Only 18 microL of total reaction solution is enough for the detection. P-LE-FCA has some merits such as lower cost, simple operation procedure and micro determination. It has been used for the determination of pyruvic acid content in human urine samples.
Zhao
Yuan-Yuan
YY
School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
Gao
Xiu-Feng
XF
Li
Yong-Sheng
YS
Ju
Xiang
X
Zhang
Jia
J
Zheng
Jia
J
D005470:Fluorometry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008832:Microchemistry; D009243:NAD / Q000378:metabolism; D019289:Pyruvic Acid / Q000652:urine*
D009243:NAD; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase