Fluorescent probes for chemical transformations on the single-molecule level.
We highlight our recent achievements in the design of fluorescent dyes for the investigation of chemical reactions on the single-molecule level. These fluorophores are tailored to undergo changes in their photophysical properties upon chemical transformations. Three examples are presented: electrophilic aromatic substitution, phosphoester cleavage, and oxidation of double-bonds. Thin-layer chromatography and fluorescence correlation spectroscopy are used to separate and characterize the different reaction products, respectively. We are planning to develop more fluorescent synthons which enable us to perform single-molecule chemistry of various reactions.
Jung
Gregor
G
Saarland University, Biophysical Chemistry, Campus, Building B2 2, Saarbruecken, Germany. g.jung@mx.uni-saarland.de
Schmitt
Alexander
A
Jacob
Michaela
M
Hinkeldey
Babette
B
D000469:Alkaline Phosphatase / Q000737:chemistry; D001896:Boron Compounds; D001966:Bromine / Q000737:chemistry; D002138:Calibration; D002621:Chemistry / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology*; D006900:Hydroxylation; D008956:Models, Chemical; D010100:Oxygen / Q000737:chemistry; D010777:Photochemistry / Q000379:methods; D011485:Protein Binding; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods
C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D000469:Alkaline Phosphatase; D010100:Oxygen; D001966:Bromine