title: Cyclic nucleotide-specific phosphodiesterases of Plasmodium falciparum: PfPDEalpha, a non-essential cGMP-specific PDE that is an integral membrane protein.
abstract: Cyclic nucleotide-specific phosphodiesterases (PDEs) have come into focus as interesting potential targets for PDE inhibitor-based anti-parasitic drugs. Genomes of the various agents of human malaria, most notably Plasmodium falciparum, all contain four genes for class 1 PDEs. The catalytic domains of these enzymes are closely related to those of the 11 human PDE families. This presents the possibility that the available vast expertise in developing drugs against human PDEs might now also be applied to developing compounds that are active against malarial PDEs. The current study identifies four Plasmodium genes that code for PfPDEalpha, PfPDEbeta, PfPDEgamma and PfPDEdelta, respectively. It further demonstrates that the PfPDEalpha polypeptide exists in two versions (PfPDEalphaA and PfPDEalphaB) that are generated by alternative splicing of the primary transcript. All malarial PDEs contain several transmembrane helices in their N-terminal regions, indicating that they are integral membrane proteins. In agreement with this prediction, essentially all PDE activity is associated with the cell membranes. PfPDEalpha was characterized as a cGMP-specific PDE that is not sensitive to a number of standard PDE inhibitors. Genetic ablation of the PfPDE1 gene produced no major phenotype in erythrocyte cultures.
authors.affiliation: Institute of Cell Biology, University of Bern, Switzerland.
authors.forename: Laurent
authors.initials: L
authors.lastname: Wentzinger
authors.forename: Selina
authors.initials: S
authors.lastname: Bopp
authors.forename: Hermann
authors.initials: H
authors.lastname: Tenor
authors.forename: Juergen
authors.initials: J
authors.lastname: Klar
authors.forename: Reto
authors.initials: R
authors.lastname: Brun
authors.forename: Hans Peter
authors.initials: HP
authors.lastname: Beck
authors.forename: Thomas
authors.initials: T
authors.lastname: Seebeck
mesh_terms: D000818:Animals; D015139:Blotting, Southern; D006152:Cyclic GMP / Q000235:genetics; D004791:Enzyme Inhibitors; D015971:Gene Expression Regulation, Enzymologic; D008288:Malaria / Q000201:enzymology / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D008969:Molecular Sequence Data; D010727:Phosphoric Diester Hydrolases / Q000235:genetics* / Q000378:metabolism; D010963:Plasmodium falciparum / Q000201:enzymology* / Q000235:genetics; D016133:Polymerase Chain Reaction; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D004791:Enzyme Inhibitors; D008565:Membrane Proteins; D015800:Protozoan Proteins; D010727:Phosphoric Diester Hydrolases; D006152:Cyclic GMP