Production of a specific extracellular inhibitor of TRPM3 channels.
OBJECTIVE
Isoform-specific ion channel blockers are useful for target validation in drug discovery and can provide the basis for new therapeutic agents and aid in determination of physiological functions of ion channels. The aim of this study was to generate a specific blocker of human TRPM3 channels as a tool to help investigations of this member of the TRP cationic channel family.


METHODS
A polyclonal antibody (TM3E3) was made to a conserved peptide of the third extracellular (E3) loop of TRPM3 and tested for binding and functional effect. Studies of channel activity were made by whole-cell planar patch-clamp and fura-2 intracellular Ca(2+) measurement.


RESULTS
Ionic current mediated by TRPM3 was inhibited partially by TM3E3 over a period of 5-10 min. Ca(2+) entry in TRPM3-expressing cells was also partially inhibited by TM3E3 in a peptide-specific manner and independently of the type of agonist used to activate TRPM3. TM3E3 had no effect on TRPC5, TRPV4, TRPM2 or an endogenous ATP response.


CONCLUSIONS
The data show the successful development of a specific TRPM3 inhibitor and give further confidence in E3 targeting as an approach to producing isoform-specific ion channel blockers.
Naylor
J
J
Institute of Membrane and Systems Biology, Faculty of Biological Sciences, University of Leeds, Leeds, UK.
Milligan
C J
CJ
Zeng
F
F
Jones
C
C
Beech
D J
DJ
D000906:Antibodies / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D005456:Fluorescent Dyes; D016257:Fura-2 / Q000378:metabolism; D006801:Humans; D007668:Kidney / Q000378:metabolism; D018408:Patch-Clamp Techniques / Q000379:methods; D011485:Protein Binding; D050053:TRPM Cation Channels / Q000037:antagonists & inhibitors*
D000906:Antibodies; D005456:Fluorescent Dyes; D050053:TRPM Cation Channels; C475667:TRPM3 protein, human; D002118:Calcium; D016257:Fura-2