Mechanism of humoral and cellular immune modulation provided by porcine sertoli cells.
The understanding of main mechanisms that determine the ability of immune privilege related to Sertoli cells (SCs) will provide clues for promoting a local tolerogenic environment. In this study, we evaluated the property of humoral and cellular immune response modulation provided by porcine SCs. Porcine SCs were resistant to human antibody and complement-mediated formation of the membrane attack complex (38.41+/-2.77% vs. 55.02+/-5.44%, p=0.027) and cell lysis (42.95+/-1.75% vs. 87.99 +/-2.25%, p<0.001) compared to immortalized aortic endothelial cells, suggesting that porcine SCs are able to escape cellular lysis associated with complement activation by producing one or more immunoprotective factors that may be capable of inhibiting membrane attack complex formation. On the other hand, porcine SCs and their culture supernatant suppressed the up-regulation of CD40 expression (p<0.05) on DCs in the presence of LPS stimulation. These novel findings, as we know, suggest that immune modulatory effects of porcine SCs in the presence of other antigen can be obtained from the first step of antigen presentation. These might open optimistic perspectives for the use of porcine SCs in tolerance induction eliminating the need for chronic immunosuppressive drugs.
Lee
Hak-Mo
HM
Xenotransplantation Research Center, Seoul National University, Seoul, Korea.
Oh
Byoung Chol
BC
Lim
Dong-Pyo
DP
Lee
Dong-Sup
DS
Lim
Hong-Gook
HG
Park
Chun Soo
CS
Lee
Jeong Ryul
JR
D000818:Animals; D000910:Antibodies, Heterophile / Q000276:immunology; D000917:Antibody Formation / Q000276:immunology*; D001011:Aorta / Q000166:cytology; D019013:CD40 Antigens / Q000276:immunology; D002461:Cell Line, Transformed; D002470:Cell Survival / Q000276:immunology; D015938:Complement Membrane Attack Complex / Q000276:immunology; D003165:Complement System Proteins / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D042783:Endothelial Cells / Q000166:cytology / Q000276:immunology; D000939:Epitopes / Q000276:immunology; D006801:Humans; D007108:Immune Tolerance / Q000276:immunology*; D007111:Immunity, Cellular / Q000276:immunology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D012708:Sertoli Cells / Q000166:cytology / Q000276:immunology*; D013552:Swine; D023822:Tissue Engineering; D014183:Transplantation, Heterologous
D000910:Antibodies, Heterophile; D019013:CD40 Antigens; D015938:Complement Membrane Attack Complex; D000939:Epitopes; D003165:Complement System Proteins