title: Desorption of Trichoderma reesei cellulase from cellulose by a range of desorbents.
abstract: The desorption of Trichoderma reesei cellulase from Avicel by a wide range of desorbents was measured. Emphasis was placed on desorption at alkaline pH. A maximum desorption of 65-68% Avicelase activity was achieved by contact with NaOH, pH 10.0, at 40 degrees C for 5 min in the presence of 0.005% Triton X-100 or Tween 80. The design of a suitable desorption process using these conditions is discussed. Glycerol was also effective as a desorbent either alone or in combination with alkali and detergent. However, relatively high concentrations of glycerol were needed and the maximum desorption achieved, 68%, was not significantly greater than that with only alkali and detergent.
authors.affiliation: Department of Chemicals and Materials Engineering, University of Auckland, Auckland, New Zealand.
authors.forename: D E
authors.initials: DE
authors.lastname: Otter
authors.forename: P A
authors.initials: PA
authors.lastname: Munro
authors.forename: G K
authors.initials: GK
authors.lastname: Scott
authors.forename: R
authors.initials: R
authors.lastname: Geddes