Characterization of antibody responses against a neutralizing epitope on the glycoprotein E2 of classical swine fever virus.
The sequence TAVSPTTLR is a conserved and linear neutralizing epitope on the glycoprotein E2 of classical swine fever virus. In this study, TAVSPTTLR-directed antibodies, induced either by virions or by an epitope-focused immunogen, were characterized. The results revealed that despite the same epitope specificity, the antibodies induced by different immunogens varied significantly both in the neutralizing test and in binding inhibition assays. This suggests that the protective immunity induced by this epitope is due to more than simply the epitope specificity and that this epitope might need essential contributions from its flanking context to induce functional epitope-specific antibodies.
Qi
Y
Y
Laboratory of Immunology, Department of Biology, Tsinghua University, Protein Science Laboratory of MOE, Beijing, People's Republic of China.
Liu
L C
LC
Zhang
B Q
BQ
Shen
Z
Z
Wang
J
J
Chen
Y H
YH
D017502:Adenovirus E2 Proteins / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000235:genetics / Q000276:immunology*; D000917:Antibody Formation / Q000276:immunology; D000918:Antibody Specificity; D006691:Classical Swine Fever / Q000276:immunology* / Q000821:virology; D006692:Classical Swine Fever Virus / Q000235:genetics / Q000276:immunology*; D000939:Epitopes / Q000737:chemistry* / Q000235:genetics / Q000276:immunology; D013552:Swine
D017502:Adenovirus E2 Proteins; D000914:Antibodies, Viral; D000939:Epitopes