Identification of proteomic biomarkers of preeclampsia in amniotic fluid using SELDI-TOF mass spectrometry.
OBJECTIVE
To identify proteomic biomarkers in amniotic fluid (AF) that can distinguish preeclampsia (PE) from chronic hypertension (CHTN) and normotensive controls (CTR).


METHODS
AF from women with PE, CHTN, and CTR were subjected to proteomic analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry.


RESULTS
Proteomic profiling of AF identified 2 biomarkers: peak X (17399.11 Da), which distinguished PE from CTR, and peak Y (28023.34 Da), which distinguished PE and CHTN from CTR. High performance liquid chromatography fractions containing the biomarkers were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in-gel tryptic digestion. The biomarkers were matched to proapolipoprotein A-I (peak Y) and a functionally obscure peptide, SBBI42 (peak X). Western blot analysis confirmed that AF from PE and CHTN had higher proapolipoprotein A-I levels than CTR.


CONCLUSIONS
Proteomic analysis of AF can distinguish PE from CHTN and CTR. The discriminatory proteins were identified as proapolipoprotein A-I and SBBI42.
Park
Joong Shin
JS
Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, Korea. jsparkmd@snu.ac.kr
Oh
Kyoung-Jin
KJ
Norwitz
Errol R
ER
Han
Joong-Soo
JS
Choi
Hye-Jin
HJ
Seong
Hyo Suk
HS
Kang
Yoon Dan
YD
Park
Chan-Wook
CW
Kim
Byoung Jae
BJ
Jun
Jong Kwan
JK
Syn
Hee Chul
HC
D000328:Adult; D000653:Amniotic Fluid / Q000737:chemistry* / Q000378:metabolism; D016632:Apolipoprotein A-I / Q000032:analysis*; D015415:Biomarkers / Q000032:analysis; D003937:Diagnosis, Differential; D005260:Female; D006801:Humans; D006973:Hypertension / Q000175:diagnosis / Q000378:metabolism; D007231:Infant, Newborn; D010865:Pilot Projects; D011225:Pre-Eclampsia / Q000175:diagnosis / Q000378:metabolism*; D011247:Pregnancy; D040901:Proteomics / Q000379:methods*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
D016632:Apolipoprotein A-I; D015415:Biomarkers