title: Clear cell odontogenic carcinoma: case report with immunohistochemical findings adding support to the challenging diagnosis.
abstract: Clear cell odontogenic carcinoma (CCOC) is a rare odontogenic tumor associated with aggressive clinical behavior, metastasis, and low survival. We report a case of CCOC affecting the mandible of a 39-year-old man. The tumor presented a biphasic pattern composed of clear cell nests intermingled with eosinophilic cells and separated by collagenous stroma. Immunoreactivity to cytokeratin (CK), specifically AE1/AE3 and CK 8, 14, 18, and 19 was found, as well as to epithelial membrane antigen (EMA). The tumor cells were negative for S100 protein, CK 13, vimentin, smooth muscle actin, laminin and type IV collagen. Low labeling indices for the proliferation markers Ki-67 and proliferating cell nuclear antigen and to p53 protein might predict a favorable prognosis for the lesion. A surgical resection was performed, followed by adjuvant radiotherapy. A 2-year follow-up has shown no signs of recurrence. The significance of histochemical and immunohistochemical resources in the correct diagnosis of CCOC is analyzed.
authors.affiliation: Department of Stomatology, Dental School, University of São Paulo, São Paulo, Brazil. f.calo@usp.br
authors.forename: Flávia Caló Aquino
authors.initials: FC
authors.lastname: Xavier
authors.forename: Camila Oliveira
authors.initials: CO
authors.lastname: Rodini
authors.forename: Luciana Maria Pedreira
authors.initials: LM
authors.lastname: Ramalho
authors.forename: Viviane Almeida
authors.initials: VA
authors.lastname: Sarmento
authors.forename: Fabio Daumas
authors.initials: FD
authors.lastname: Nunes
authors.forename: Suzana Cantanhede Orsini Machado
authors.initials: SC
authors.lastname: de Sousa
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000473:pathology*; D000328:Adult; D006801:Humans; D007633:Keratins / Q000032:analysis; D008297:Male; D008339:Mandibular Neoplasms / Q000473:pathology*; D018396:Mucin-1 / Q000032:analysis; D009808:Odontogenic Tumors / Q000473:pathology*
chemical_list: D018396:Mucin-1; D007633:Keratins