MR tracking of transplanted cells with "positive contrast" using manganese oxide nanoparticles.
Rat glioma cells were labeled using electroporation with either manganese oxide (MnO) or superparamagnetic iron oxide (SPIO) nanoparticles. The viability and proliferation of SPIO-labeled cells (1.9 mg Fe/ml) or cells electroporated with a low dose of MnO (100 microg Mn/ml) was not significantly different from unlabeled cells; a higher MnO dose (785 microg Mn/ml) was found to be toxic. The cellular ion content was 0.1-0.3 pg Mn/cell and 4.4 pg Fe/cell, respectively, with cellular relaxivities of 2.5-4.8 s(-1) (R(1)) and 45-84 s(-1) (R(2)) for MnO-labeled cells. Labeled cells (SPIO and low-dose MnO) were each transplanted in contralateral brain hemispheres of rats and imaged in vivo at 9.4T. While SPIO-labeled cells produced a strong "negative contrast" due to the increase in R(2), MnO-labeled cells produced "positive contrast" with an increased R(1). Simultaneous imaging of both transplants with opposite contrast offers a method for MR "double labeling" of different cell populations.
Gilad
Assaf A
AA
Russell H. Morgan Department of Radiology, Division of MR Research, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA.
Walczak
Piotr
P
McMahon
Michael T
MT
Na
Hyon Bin
HB
Lee
Jung Hee
JH
An
Kwangjin
K
Hyeon
Taegwhan
T
van Zijl
Peter C M
PC
Bulte
Jeff W M
JW
D000818:Animals; D017690:Cell Transplantation; D003287:Contrast Media; D018274:Electroporation; D005290:Ferric Compounds; D005910:Glioma; D008279:Magnetic Resonance Imaging / Q000379:methods*; D017895:Manganese Compounds; D053768:Metal Nanoparticles; D010087:Oxides; D051381:Rats; D011916:Rats, Inbred F344; D014407:Tumor Cells, Cultured
D003287:Contrast Media; D005290:Ferric Compounds; D017895:Manganese Compounds; D010087:Oxides; C000499:ferric oxide; C027424:manganese oxide