title: Canine parvovirus 2c infection in central Portugal.
abstract: Canine parvovirus (CPV) has been evolving, generating new genetic and antigenic variants throughout the world. This study was conducted to determine the types of CPV circulating in dogs in Figueira da Foz, Portugal. Thirty fecal samples, collected between 2006 and 2007 from dogs with clinical signs of CPV infection, were tested for CPV by a rapid, in-clinic, enzyme-linked immunosorbent assay (ELISA)/immunomigration test, by conventional real-time polymerase chain reaction (PCR), and by minor-groove binding TaqMan PCR. Of the 29 PCR-positive samples, 15 were identified as CPV-2b and 14 as CPV-2c. No CPV-2a was detected. The sensitivity of the ELISA test was 82.76% compared with the PCR assays. No significant associations were found between CPV type, clinical outcome, breed, vaccination status, or age.
authors.affiliation: Institute of Biomedical Sciences Abel Salazar, University of Porto, Portugal.
authors.forename: Maria
authors.initials: M
authors.lastname: João Vieira
authors.forename: Eliane
authors.initials: E
authors.lastname: Silva
authors.forename: João
authors.initials: J
authors.lastname: Oliveira
authors.forename: Ana
authors.initials: A
authors.lastname: Luísa Vieira
authors.forename: Nicola
authors.initials: N
authors.lastname: Decaro
authors.forename: Costantina
authors.initials: C
authors.lastname: Desario
authors.forename: Alexandra
authors.initials: A
authors.lastname: Muller
authors.forename: Júlio
authors.initials: J
authors.lastname: Carvalheira
authors.forename: Canio
authors.initials: C
authors.lastname: Buonavoglia
authors.forename: Gertrude
authors.initials: G
authors.lastname: Thompson
mesh_terms: D000818:Animals; D004283:Dog Diseases / Q000453:epidemiology / Q000821:virology*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D010322:Parvoviridae Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology; D017993:Parvovirus, Canine / Q000145:classification*; D016133:Polymerase Chain Reaction / Q000662:veterinary; D011174:Portugal / Q000453:epidemiology; D012680:Sensitivity and Specificity