The brain proteome profile is highly conserved between Prnp-/- and Prnp+/+ mice.
The aim of this study is to compare the proteome of Prnp-/- (Zürich I) gene-ablated mouse brains with wild-type mouse brains. Fluorescence two-dimensional-difference gel electrophoresis (DIGE) and isotope-coded protein labeling (ICPL) were applied for brain homogenates. Similar quantitative protein profiles (<or=1.5-fold change, P<0.05) were obtained by quantitative two-dimensional-DIGE analysis of whole brain homogenates of two developmental stages (days 1 and 67). Complementary ICPL-liquid chromatography-mass spectrometry experiments (n=5) of whole brain homogenates and synaptosomes were performed. The abundance ratios of all proteins quantified did not exceed the statistical spread determined (<2-fold). Therefore, both of the two-dimensional-DIGE and ICPL analyses demonstrated a highly conserved quantitative protein profile in brain homogenates of Prnp-/- and Prnp+/+ mice.
Crecelius
Anna C
AC
Laboratory of Molecular Neuropathology, Centre of Neuropathology and Prion Research, LAFUGA, Gene Centre, LMU, Munich, Germany.
Helmstetter
Derrick
D
Strangmann
Julia
J
Mitteregger
Gerda
G
Fröhlich
Thomas
T
Arnold
Georg J
GJ
Kretzschmar
Hans A
HA
D000818:Animals; D001921:Brain / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics*; D020543:Proteome / Q000378:metabolism*
D009419:Nerve Tissue Proteins; D000072002:Prion Proteins; D011328:Prions; C512657:Prnp protein, mouse; D020543:Proteome