title: Two forms of Vibrio vulnificus metalloprotease VvpE are secreted via the type II general secretion system.
abstract: Vibrio vulnificus has been known to secrete one form of metalloprotease VvpE (45 kDa) that is cleaved to 34 kDa-VvpE and 11 kDa-C-terminal propeptide via extracellular autoproteolysis. However, we found that extracellular secretion of both the 34 and 45 kDa forms of VvpE began in the early growth phase; moreover, 34 kDa-VvpE existed as the major form in V. vulnificus cell lysates and culture supernatants. In addition, extracellular secretion of both 34 and 45 kDa-VvpE was blocked by mutation of the pilD gene, which encodes for the type IV leader peptidase/N-methyltransferase of the type II general secretion system, and the blocked VvpE secretion was recovered by in trans-complementation of the wild-type pilD gene. These results indicate that 34 kDa-VvpE is the major form secreted along with 45 kDa-VvpE from the early growth phase via the PilD-mediated type II general secretion system.
authors.affiliation: Research Center for Resistant Cells, Chosun University Medical School, Gwangju, Republic of Korea.
authors.forename: Jong
authors.initials: J
authors.lastname: Park
authors.forename: So-Yeon
authors.initials: SY
authors.lastname: Ryu
authors.forename: Choon-Mee
authors.initials: CM
authors.lastname: Kim
authors.forename: Sung-Heui
authors.initials: SH
authors.lastname: Shin
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005110:Extracellular Space / Q000201:enzymology; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008666:Metalloendopeptidases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008780:Methyltransferases / Q000235:genetics / Q000378:metabolism; D021381:Protein Transport; D012697:Serine Endopeptidases / Q000235:genetics / Q000378:metabolism; D014735:Vibrio Infections / Q000382:microbiology; D041261:Vibrio vulnificus / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D008780:Methyltransferases; D012697:Serine Endopeptidases; C026515:type I signal peptidase; D008666:Metalloendopeptidases; C070101:vvP protein, Vibrio vulnificus