title: [Determination of vetatramine in Veratrum nigrum by HPLC-ELSD].
abstract: OBJECTIVE
  To develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum.
  
  
  METHODS
  The analy fical column was Shim-pack ODS - C18 (4.6 mm x 250 mm, 4 microm) column, the mobile phase was acetonitrile-water (containing 0.1% triethylamine) (50:50), at a flow rate of 0.8 mL x min(-1). The temperature of drift tube was 90 degrees C and the gas flow was at the rate of 2.5 L x min(-1).
  
  
  RESULTS
  The calibration curve was linear in the range of 0.36-3.6 microg (r = 0.999 8). The average recovery was 100.9% (RSD 2.3%, n = 6). The contents of veratramine in Veratrum nigrum. from the ten different sources were determined.
  
  
  CONCLUSIONS
  The method may be used as a accurate and reproducible way to determine the content of veratramine in V. nigrum.
authors.affiliation: College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China. wls6856@163.com
authors.forename: Li-Shu
authors.initials: LS
authors.lastname: Wang
authors.forename: Da-Qing
authors.initials: DQ
authors.lastname: Zhao
authors.forename: Hua-Ming
authors.initials: HM
authors.lastname: Tao
authors.forename: Yong-Hong
authors.initials: YH
authors.lastname: Liu
mesh_terms: D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014703:Veratrum / Q000737:chemistry*; D014704:Veratrum Alkaloids / Q000032:analysis* / Q000302:isolation & purification
chemical_list: D004365:Drugs, Chinese Herbal; D014704:Veratrum Alkaloids; C009649:veratramine