title: Relation between menin expression and NF-kappaB activity in an intestinal cell line.
abstract: In a previous study, we demonstrated that the Men1 gene is mainly expressed in the proliferative crypt compartment of the small intestine and that a reduction of menin expression in the crypt-like IEC-17 cell line induces an increase in proliferation rate concomitant with an increase in cyclin D1 expression. The aim of the present study was to test the hypothesis that the NF-kappaB pathway may be involved in cyclin D1 overexpression. Transcriptional activity of the cyclin D1 gene promoter was increased upon reduction of menin expression. Blockade of the NF-kappaB pathway restored proliferation, cell cycle, cyclin D1 gene transcription and cyclin D1 expression levels to those observed in the presence of menin. These data support a correlation between cyclin D1 expression, NF-kappaB activity and menin expression in this epithelial cell line and are relevant to the physiological function of menin in regulating proliferation in the intestinal epithelium.
authors.affiliation: Inserm, U865, IFR Lyon Est, Faculté Laënnec, 69372 Lyon Cedex 8, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Theillaumas
authors.forename: Martine
authors.initials: M
authors.lastname: Blanc
authors.forename: Christophe
authors.initials: C
authors.lastname: Couderc
authors.forename: Gilles
authors.initials: G
authors.lastname: Poncet
authors.forename: Wissam
authors.initials: W
authors.lastname: Bazzi
authors.forename: Christine
authors.initials: C
authors.lastname: Bernard
authors.forename: Martine
authors.initials: M
authors.lastname: Cordier-Bussat
authors.forename: Jean Yves
authors.initials: JY
authors.lastname: Scoazec
authors.forename: Colette
authors.initials: C
authors.lastname: Roche
mesh_terms: D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D004386:Duodenum / Q000166:cytology / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: C105373:MEN1 protein, human; C532041:Men1 protein, rat; D011518:Proto-Oncogene Proteins; D051996:Transcription Factor RelA; D014157:Transcription Factors; D019938:Cyclin D1