title: Protective activity of the Bordetella pertussis BrkA autotransporter in the murine lung colonization model.
abstract: This study examined the vaccine potential of the autotransporter protein BrkA of Bordetella pertussis in the sublethal intranasal murine respiratory challenge model of infection. Five different acellular pertussis (Pa) vaccines, containing different pertussis-component antigens but all comprizing diphtheria (D) and tetanus (T) toxoids, were tested. A two-pertussis-component DTPa vaccine containing pertussis toxoid (PT) and filamentous hemagglutinin (FHA) induced only limited bacterial clearance. However, a three-pertussis-component DTPa vaccine containing PT, FHA and a recombinant BrkA protein (rBrkA) was found to be as efficacious in protecting mice against colonization by B. pertussis strains Tohama I and 18-323 as the commercial Infanrixtrade mark vaccine that also includes PT and FHA but pertactin (PRN) instead of rBrkA. Vaccination of mice with rBrkA as the only B. pertussis antigen did not protect against colonization by B. pertussis. We also demonstrated that BrkA is ubiquitously expressed by highly prevalent clinical isolates of B. pertussis and suggest that new acellular pertussis vaccine formulations that include BrkA have equivalent efficacy as currently available DTPa vaccines against B. pertussis infections.
authors.affiliation: Department of Microbiology and Immunology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3.
authors.forename: Nico
authors.initials: N
authors.lastname: Marr
authors.forename: David C
authors.initials: DC
authors.lastname: Oliver
authors.forename: Vincianne
authors.initials: V
authors.lastname: Laurent
authors.forename: Jan
authors.initials: J
authors.lastname: Poolman
authors.forename: Philippe
authors.initials: P
authors.lastname: Denoël
authors.forename: Rachel C
authors.initials: RC
authors.lastname: Fernandez
mesh_terms: D018829:Adhesins, Bacterial / Q000276:immunology; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000276:immunology*; D001886:Bordetella pertussis / Q000254:growth & development / Q000276:immunology*; D015169:Colony Count, Microbial; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines / Q000276:immunology; D005260:Female; D008168:Lung / Q000276:immunology* / Q000382:microbiology; D051379:Mice; D014121:Toxoids / Q000276:immunology; D010566:Virulence Factors, Bordetella / Q000276:immunology; D014917:Whooping Cough / Q000517:prevention & control*
chemical_list: D018829:Adhesins, Bacterial; D001425:Bacterial Outer Membrane Proteins; C090007:BrkA protein, Bordetella pertussis; D022681:Diphtheria-Tetanus-acellular Pertussis Vaccines; D014121:Toxoids; D010566:Virulence Factors, Bordetella; C065273:filamentous hemagglutinin adhesin, Bordetella pertussis; C051093:pertussis toxoid