title: Lymphocyte and cytokines after short periods of exercise.
abstract: The purpose of this study was to verify the effects of short periods of exercise of different intensity on lymphocyte function and cytokines. Thirty Wistar rats, 2 months old, were used. They were divided into five groups of six rats: a sedentary control group; a group exercised for 5 minutes at low intensity (5 L); a group exercised for 15 minutes at low intensity (15 L); and groups exercised at moderate intensity (additional load of 5 % of body weight) for 5 minutes (5 M) or for 15 minutes (15 M). The parameters measured were: total leukocytes, neutrophils, lymphocytes, monocytes, lymphocytes from lymph nodes, serum cytokines (IL-2, IL-6 and TNF-alpha), lymphocyte mitochondrial transmembrane potential, viability and DNA fragmentation. ANOVA two way followed by Tukey's post hoc test (p <or= 0.05) was used. The exercised groups exhibited a significant increase in total leukocytes, tissue and circulating lymphocytes in comparison with the control group. There was a significant decrease in lymphocyte viability and decrease in DNA fragmentation for the 15 M group when compared with the control. There was a decrease in the level TNF-alpha in the 5 M and 15 M groups. Short-term, low- and moderate-intensity exercise may be considered for sedentary individuals beginning to exercise, since no deleterious alterations were observed in lymphocyte function.
authors.affiliation: Health Science Faculty, Methodist University of Piracicaba, Piracicaba, Brazil. jonatop@gmail.com
authors.forename: J
authors.initials: J
authors.lastname: Prestes
authors.forename: C K O
authors.initials: CK
authors.lastname: de Ferreira
authors.forename: R
authors.initials: R
authors.lastname: Dias
authors.forename: A B
authors.initials: AB
authors.lastname: Frollini
authors.forename: F F
authors.initials: FF
authors.lastname: Donatto
authors.forename: M F
authors.initials: MF
authors.lastname: Cury-Boaventura
authors.forename: M G
authors.initials: MG
authors.lastname: Guereschi
authors.forename: T C
authors.initials: TC
authors.lastname: Pithon-Curi
authors.forename: R
authors.initials: R
authors.lastname: Verlengia
authors.forename: A C
authors.initials: AC
authors.lastname: Palanch
authors.forename: R
authors.initials: R
authors.lastname: Curi
authors.forename: C R
authors.initials: CR
authors.lastname: Cavaglieri
mesh_terms: D000818:Animals; D017209:Apoptosis; D016207:Cytokines / Q000097:blood*; D006801:Humans; D007958:Leukocyte Count; D008214:Lymphocytes; D009000:Monocytes; D009504:Neutrophils; D010805:Physical Conditioning, Animal / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha
chemical_list: D016207:Cytokines; D014409:Tumor Necrosis Factor-alpha