New steroidal glycosides from rhizomes of Clintonia udensis.
A total of 10 steroidal glycosides, together with three new spirostanol glycosides (6-8), a new furostanol glycoside (9), and a new cholestane glycoside (10), were isolated from the rhizomes of Clintonia udensis (Liliaceae). The structures of the new compounds were determined on the basis of extensive spectroscopic analyses, including 2-D nuclear magnetic resonance (NMR) data, and of hydrolytic cleavage followed by chromatographic or spectroscopic analyses. The isolated glycosides were evaluated for their cytotoxic activity against HL-60 leukemia cells. Spirostanol glycosides 1 and 2, and furostanol glycoside 4 showed cytotoxic activity with IC(50) values of 3.2+/-0.02, 2.2+/-0.12, and 2.2+/-0.06 microg/ml, respectively. Neither the spirostanol and furostanol saponins with a hydroxy group at C-1 (6 and 9) and C-12 (7 and 8) nor cholestane glycosides (5 and 10) exhibited apparent cytotoxic activity at a sample concentration of 10 microg/ml.
Matsuo
Yukiko
Y
Tokyo University of Pharmacy and Life Sciences, School of Pharmacy, Hachiouji, Tokyo, Japan.
Watanabe
Kazuki
K
Mimaki
Yoshihiro
Y
D000970:Antineoplastic Agents / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use; D018922:HL-60 Cells; D006801:Humans; D019668:Liliaceae / Q000737:chemistry*; D015394:Molecular Structure; D027343:Rhizome / Q000737:chemistry*; D013256:Steroids / Q000737:chemistry* / Q000302:isolation & purification / Q000627:therapeutic use
D000970:Antineoplastic Agents; D006027:Glycosides; D013256:Steroids