title: CLASP regulates mitochondrial distribution in Schizosaccharomyces pombe.
abstract: Movement of mitochondria in Schizosaccharomyces pombe depends on their association with the dynamic, or plus ends, of microtubules, yet the molecular basis for this interaction is poorly understood. We identified mmd4 in a screen of temperature-sensitive S. pombe strains for aberrant mitochondrial morphology and distribution. Cells with the mmd4 mutation display mitochondrial aggregation near the cell ends at elevated temperatures, a phenotype similar to mitochondrial defects observed in wild-type cells after microtubule depolymerization. However, microtubule morphology and function appear normal in the mmd4 mutant. The mmd4 lesion maps to peg1(+), which encodes a microtubule-associated protein with homology to cytoplasmic linker protein-associated proteins (mammalian microtubule plus end-binding proteins). Peg1p localizes to the plus end of microtubules and to mitochondria and is recovered with mitochondria during subcellular fractionation. This mitochondrial-associated fraction of Peg1p displays properties of a peripherally associated protein. Peg1p is the first identified microtubule plus end-binding protein required for mitochondrial distribution and likely functions as a molecular link between mitochondria and microtubules.
authors.affiliation: Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA. schiron@gmail.com
authors.forename: Stéphane
authors.initials: S
authors.lastname: Chiron
authors.forename: Alyona
authors.initials: A
authors.lastname: Bobkova
authors.forename: Haowen
authors.initials: H
authors.lastname: Zhou
authors.forename: Michael P
authors.initials: MP
authors.lastname: Yaffe
mesh_terms: D000483:Alleles; D016764:Cell Polarity; D005800:Genes, Fungal; D007399:Interphase; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D008870:Microtubules / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D009154:Mutation / Q000235:genetics; D009687:Nuclear Proteins / Q000378:metabolism*; D053140:Organelle Shape; D010641:Phenotype; D021381:Protein Transport; D012568:Schizosaccharomyces / Q000166:cytology / Q000235:genetics / Q000378:metabolism*; D029702:Schizosaccharomyces pombe Proteins / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; C513960:Peg1 protein, S pombe; D029702:Schizosaccharomyces pombe Proteins