Bile components and amino acids affect survival of the newly excysted juvenile Clonorchis sinensis in maintaining media.
Clonorchis sinensis thrives on bile juice. The effects of bile and bile acids on newly excysted juvenile C. sinensis (CsNEJ) were studied in terms of survival. Survival of CsNEJs maintained in 1x Locke's solution, Dulbecco's modified Eagle's medium, NCTC 109, Eagle's, RPMI 1640, and 0.1% glucose was high, but dropped rapidly in 2x Locke's, 0.85% NaCl, and phosphate-buffered saline. Most amino acids in the media favored CsNEJ survival; however, aspartic and glutamic acids and adenine reduced survival. Survival was also significantly lower in media containing more than 0.1% bile. CsNEJs preconditioned in low bile media survived longer in higher bile media. All bile acids and conjugated bile salts were found to favor CsNEJ survival, except for lithocholic acid (LCA) which was toxic. NCTC 109 medium was found to be optimal for the in vitro maintenance of CsNEJs and 1x Locke's solution to be suitable for analyzing the biological effects of bioactive compounds and molecules. Based on these results, we propose that bile acids enhance activity of CsNEJs, but LCA deteriorate CsNEJs.
Li
Shunyu
S
Department of Medical Environmental Biology, Chung-Ang University College of Medicine, Tongjak-gu, Seoul, 156-756, Republic of Korea.
Kim
Tae Im
TI
Yoo
Won Gi
WG
Cho
Pyo Yun
PY
Kim
Tong-Soo
TS
Hong
Sung-Jong
SJ
D000596:Amino Acids / Q000032:analysis / Q000494:pharmacology*; D000818:Animals; D001647:Bile Acids and Salts / Q000032:analysis / Q000494:pharmacology*; D003004:Clonorchis sinensis / Q000187:drug effects* / Q000502:physiology*; D003470:Culture Media / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D013997:Time Factors
D000596:Amino Acids; D001647:Bile Acids and Salts; D003470:Culture Media