title: Ultrasound enhanced bioprocesses: cholesterol oxidation by Rhodococcus erythropolis.
abstract: Cholesterol oxidation to cholestenone by resting cells of Rhodococcus erythropolis ATCC25544 was investigated under a computer-controlled ultrasonic irradiation at a frequency of 20 kHz. The optimization of the ultrasound intensity and its mode of application to a stirred bioreactor was first established at a level which preserved the structural integrity of the cells and enabled their metabolic activity. A significant enhancement in the kinetic rates of the biotransformation was observed in microbial slurries of 1.0 and 2.5 g/L cholesterol when sonicated for 5 s every 10 min with a power output of 2.2 W/cm(2). In contrast, ultrasound had no effect on the enzymatic oxidation of cholesterol (2.5 g/L) by cholesterol oxidase. A high loading of cholesterol (5.0 g/L) in sonicated microbial systems had, however, an adverse effect. The ultrasound enhancement is discussed in terms of an increased dissolution rate of the sustrate crystals and more importantly, in terms of the uniquely ultrasound-induced enhancement of mass transfer inside and outside a cell.
authors.affiliation: Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22901, USA.
authors.forename: R
authors.initials: R
authors.lastname: Bar