Pulsed diode laser-based monitor for singlet molecular oxygen.
Photodynamic therapy (PDT) is a promising cancer treatment. PDT uses the affinity of photosensitizers to be selectively retained in malignant tumors. When tumors, pretreated with the photosensitizer, are irradiated with visible light, a photochemical reaction occurs and tumor cells are destroyed. Oxygen molecules in the metastable singlet delta state O2(1Delta) are believed to be the species that destroys cancerous cells during PDT. Monitoring singlet oxygen produced by PDT may lead to more precise and effective PDT treatments. Our approach uses a pulsed diode laser-based monitor with optical fibers and a fast data acquisition system to monitor singlet oxygen during PDT. We present results of in vitro singlet oxygen detection in solutions and in a rat prostate cancer cell line as well as PDT mechanism modeling.
Lee
Seonkyung
S
Physical Sciences Inc., 20 New England Business Center, Andover, Massachusetts 01810-1077, USA. lee@psicorp.com
Zhu
Leyun
L
Minhaj
Ahmed M
AM
Hinds
Michael F
MF
Vu
Danthu H
DH
Rosen
David I
DI
Davis
Steven J
SJ
Hasan
Tayyaba
T
D000818:Animals; D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005336:Fiber Optic Technology / Q000295:instrumentation*; D008027:Light; D008297:Male; D055100:Optical Fibers; D010092:Oximetry / Q000295:instrumentation*; D011471:Prostatic Neoplasms / Q000378:metabolism*; D011829:Radiation Dosage; D011874:Radiometry / Q000295:instrumentation*; D051381:Rats; D026082:Singlet Oxygen / Q000032:analysis*
D026082:Singlet Oxygen