Production of mRNA from the cry1Ac transgene differs among Bollgard lines which correlates to the level of subsequent protein.
Commercial cultivars of Bollgard cotton, Gossypium hirsutum L., differ in the amount of expressed Cry1Ac protein. However, the plant-mechanism for which this occurs is still unknown. Using quantitative real-time polymerase chain reaction (qPCR), we developed a method to determine if differences in the overall level of Cry1Ac among Bollgard lines could be correlated to the mRNA transcripts. Our data shows that the cry1Ac mRNA transcript differs among Bollgard lines and are correlated with corresponding Cry1Ac protein levels. In addition, qPCR based methods can efficiently be employed to quantify Cry1Ac protein expression levels in transgenic cotton cultivars. We postulate that qPCR based methods could be successfully employed for quantifying expression levels of transgenes in plants carrying different Bt toxins.
Adamczyk
John J
JJ
USDA, ARS, Southern Insect Management Research Unit, Stoneville, MS 38776, USA. John.Adamczyk@ars.usda.gov
Perera
Omaththage
O
Meredith
William R
WR
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D004731:Endotoxins / Q000235:genetics* / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D003368:Gossypium / Q000235:genetics* / Q000378:metabolism; D006460:Hemolysin Proteins / Q000235:genetics* / Q000378:metabolism*; D007306:Insecticides / Q000378:metabolism; D010572:Pest Control, Biological; D030821:Plants, Genetically Modified / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D019076:Transgenes / Q000502:physiology*
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004731:Endotoxins; D006460:Hemolysin Proteins; D007306:Insecticides; D012333:RNA, Messenger; C030956:insecticidal crystal protein, Bacillus Thuringiensis