[Study on the molecular typing of Enterobacter sakazakii with pulsed-field gel electrophoreses].
OBJECTIVE
To analyze the fingerprint of Enterobacter sakazakii, twenty-nine isolates and two type strains were analyzed with the pulsed-field gel electrophoreses (PFGE).


METHODS
All of strains were digested with restriction endonucleases Xba I and Spe I respectively, and the PFGE patterns were analyzed with BioNumerics software.


RESULTS
PFGE with restriction endonucleases Xba I and Spe I gave 30 kinds of PFGE patterns respectively. Twenty-nine strains of Enterobacter sakazakii gave different patterns except ES 004 and ES 005. ES 004 and ES 005, which were isolated from the infant formula with same brand and same lot but different package, showed the same PFGE pattern with endonucleases Xba I and Spe I. According to the analysis of BioNumerics software, there is no significant correlation between PFGE patterns and source information of corresponding samples except ES 004 and ES 005.


CONCLUSIONS
Though the average number of PFGE bands with Xba I was less than that with Spe I, it proved that PFGE with Xba I still has a high degree of discrimination. The above two PFGE methods both can be used to the molecular typing and tracing of Enterobacter sakazakii.
Pei
Xiaoyan
X
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention,Beijing 100050, China.
Guo
Yunchang
Y
Liu
Xiumei
X
D015373:Bacterial Typing Techniques; D044083:Cronobacter sakazakii / Q000145:classification* / Q000235:genetics; D016172:DNA Fingerprinting; D004269:DNA, Bacterial / Q000032:analysis*; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology; D006801:Humans; D007223:Infant; D041943:Infant Formula
D004269:DNA, Bacterial