Antibody detection and cross-reactivity among species and serogroups of Legionella by indirect immunofluorescence test.
The purpose of the present study was to determine possible cross-reactivity between different serogroups or between different species by means of bacterial smears of individual L. pneumophila serogroups and several non-pneumophila Legionella spp. Using BIOCHIP slide we found a considerable frequency of cross-reactions with L. pneumophila serogroup 12 (88.4%) therefore BIOCHIP slide proved as useful method for identification of cross-reactivity between members of the Legionnellaceae.
Ditommaso
S
S
Dipartimento di Sanità Pubblica e di Microbiologia, Università degli Studi di Torino, Via Santena 5 bis 10126 Torino, Italy. savina.ditommaso@unito.it
Giacomuzzi
M
M
Gentile
M
M
Zotti
C M
CM
D000907:Antibodies, Bacterial / Q000097:blood* / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology*; D003429:Cross Reactions; D019084:Fluorescent Antibody Technique, Indirect / Q000379:methods*; D006801:Humans; D007875:Legionella / Q000145:classification* / Q000276:immunology*; D016952:Legionella pneumophila / Q000145:classification / Q000276:immunology*; D007876:Legionellosis / Q000175:diagnosis / Q000276:immunology; D007877:Legionnaires' Disease / Q000175:diagnosis / Q000276:immunology; D040081:Protein Array Analysis / Q000379:methods; D012703:Serotyping
D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial