A comparison of four DNA extraction methods for the detection of Citrus yellow mosaic badna virus from two species of citrus using PCR and dot-blot hybridization.
Nucleic acid preparations extracted using four procedures were assessed to determine the suitability of the procedure for PCR-based and DNA dot-blot-based detection of Citrus yellow mosaic badna virus (CMBV) from two citrus species, acid lime and pummelo. It was found that the success of PCR detection depended upon the procedure of DNA extraction whereas the dot-blot detection was successful with all extraction methods examined. CMBV DNA sequences amplified from two citrus species indicated high nucleotide sequence identity to the sequences reported previously from sweet orange. These results will help in choosing the correct DNA extraction procedure to be followed for efficient virus screening of citrus propagules.
Borah
Basanta K
BK
Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
Anthony Johnson
A M
AM
Department of Virology, S.V. University, Tirupati, Andhra Pradesh, India.
Sai Gopal
D V R
DVR
Department of Virology, S.V. University, Tirupati, Andhra Pradesh, India.
Dasgupta
Indranil
I
Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi 110021, India.
D017796:Caulimovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D002957:Citrus / Q000821:virology*; D017931:DNA Primers; D004279:DNA, Viral / Q000235:genetics* / Q000302:isolation & purification*; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers; D004279:DNA, Viral