Effect of ivelip and sodium nitroprusside on 5-HT, glutathione and lipid peroxidation in normal and malnourished rat brain.
We examined the effect of experimental malnutrition and diet supplementation of parameters of central nervous system damage. Wistar rats were fed during 30 days and classified as malnourished (MN, 7% protein content diet) or well-nourished (WN, 23% protein content diet), were grouped and treated as follows: I-control; II-SNP (20 microg/kg); IIl-Ivelip (280 mg/kg) and IV-Ivelip + sodium nitroprusside (SNP). Levels of lipid peroxidation (TBARS), glutathione (GSH), tryptophan (Trp) and serotonin (5-HT) were assessed in brain by liquid chromatography. TBARS and GSH levels increased significantly (p < 0.05) in MN vs. WN rats that did not receive Ivelip. No significant differences were observed in TBARS and GSH among rats that received Ivelip or SNP. The weight of rats decreased significantly (p < 0.05) in all MN groups in relation to the WN groups. Hemoglobin (Hb) levels increased significantly (p < 0.05) in MN and WN groups that received Ivelip. 5-HT levels increased significantly (p < 0.05) in all MN groups. Trp levels increased significantly (p < 0.05) in the WN + Ivelip group vs. control. Early malnutrition induces changes in the metabolism of biogenic amines and this condition may promote oxidative injury of the brain.
Calderón Guzmán
David
D
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría (INP), México.
Espitia Vázquez
Ivonne
I
Barragán Mejía
Gerardo
G
Osnaya Brizuela
Norma
N
Hernández García
Ernestina
E
Juárez-Olguín
Hugo
H
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001923:Brain Chemistry / Q000187:drug effects*; D004032:Diet; D005217:Fat Emulsions, Intravenous / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism*; D006454:Hemoglobins / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D044342:Malnutrition / Q000378:metabolism*; D009599:Nitroprusside / Q000494:pharmacology*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D011502:Protein-Energy Malnutrition / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012701:Serotonin / Q000378:metabolism*; D013024:Soybean Oil / Q000494:pharmacology*; D014280:Triglycerides / Q000494:pharmacology; D014364:Tryptophan / Q000378:metabolism
D005217:Fat Emulsions, Intravenous; D006454:Hemoglobins; D014280:Triglycerides; D009599:Nitroprusside; D012701:Serotonin; D013024:Soybean Oil; D014364:Tryptophan; D005978:Glutathione