Pre-analytical method for metabolic profiling of plant cell cultures of Passiflora garckei.
Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparation method. Solid phase extraction (SPE) was employed to isolate the plant metabolites for nuclear magnetic resonance (NMR) analysis and to subsequently detect the differences between yeast extract elicited and control cells. Compared with previous results obtained by using a Sephadex LH-20 column, SPE coupled with NMR spectroscopy improves the analysis of aromatic compounds e.g.: trans-feruloyl derivatives and trans-coumaroyl derivatives. Moreover, it decreases the concentration of sugars that usually overlap with many plant metabolite signals.
Fraccaroli
Marica
M
Scientific and Technological Department, Faculty of Mathematics, Physics and Natural Science, University of Verona, Verona, Italy. maricafraccaroli@gmail.com
Nicoletti
Silvia
S
Maltese
Federica
F
Choi
Young Hae
YH
Guzzo
Flavia
F
Levi
Marisa
M
Verpoorte
Robert
R
D002457:Cell Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002478:Cells, Cultured; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D029598:Passiflora / Q000166:cytology / Q000378:metabolism*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
D002457:Cell Extracts; D000432:Methanol