Protective effect of butin against hydrogen peroxide-induced apoptosis by scavenging reactive oxygen species and activating antioxidant enzymes.
The antioxidant property of butin was investigated for cytoprotective effect against H(2)O(2)-induced cell damage. This compound showed intracellular reactive oxygen species (ROS) scavenging, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, inhibition of lipid peroxidation, and DNA damage. This radical scavenging activity of butin protected cell damage exposed to H(2)O(2). Also, butin reduced the apoptotic cells induced by H(2)O(2), as demonstrated by the decreased DNA fragmentation, apoptotic body formation, and caspase 3 activity. In addition, butin restored the activity and protein expression of cellular antioxidant enzymes, superoxide dismutase (SOD), and catalase (CAT) in H(2)O(2)-treated cells. Taken together, these findings suggest that butin protected cells against H(2)O(2)-induced cell damage via antioxidant property.
Zhang
Rui
R
Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si 690-756, South Korea.
Chae
Sungwook
S
Kang
Kyoung Ah
KA
Piao
Mei Jing
MJ
Ko
Dong Ok
DO
Wang
Zhi Hong
ZH
Park
Doek Bae
DB
Park
Jae Woo
JW
You
Ho Jin
HJ
Hyun
Jin Won
JW
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D001578:Benzopyrans / Q000737:chemistry / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism; D006224:Cricetinae; D003412:Cricetulus; D019610:Cytoprotection / Q000187:drug effects*; D004249:DNA Damage; D004789:Enzyme Activation / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D016166:Free Radical Scavengers / Q000494:pharmacology*; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D013482:Superoxide Dismutase / Q000378:metabolism
D000975:Antioxidants; D001578:Benzopyrans; D016166:Free Radical Scavengers; C051437:butin; D006861:Hydrogen Peroxide; D002374:Catalase; D013482:Superoxide Dismutase