title: The predominant cultivable Veillonella spp. of the tongue of healthy adults identified using rpoB sequencing.
abstract: The predominant Veillonella spp. were isolated from the dorsum surface of the tongues of 11 healthy adults and identified to species level using rpoB sequencing because 16S ribosomal RNA sequence analysis does not reliably differentiate between all members of this genus. In all, 253 isolates were identified and the mean proportion (+/- SE) of Veillonella spp. per sample was 16.2 (+/- 3.6) with a range of 3.0% to 36.3% of the total anaerobic colony count. The predominant species were Veillonella atypica (10/11), Veillonella dispar (9/11) and Veillonella rogosae (8/11) because they were isolated from the majority of subjects. Veillonella parvula was isolated from only one subject while Veillonella dentocariosi and Veillonella montpelleriensis were not isolated from any subject.
authors.affiliation: Infection Research Group, King's College London, Dental Institute, London, UK. david.beighton@kcl.ac.uk
authors.forename: D
authors.initials: D
authors.lastname: Beighton
authors.forename: D
authors.initials: D
authors.lastname: Clark
authors.forename: B
authors.initials: B
authors.lastname: Hanakuka
authors.forename: S
authors.initials: S
authors.lastname: Gilbert
authors.forename: T
authors.initials: T
authors.lastname: Do
mesh_terms: D000328:Adult; D001426:Bacterial Proteins / Q000032:analysis*; D015169:Colony Count, Microbial; D012321:DNA-Directed RNA Polymerases / Q000032:analysis*; D006801:Humans; D012336:RNA, Ribosomal, 16S / Q000032:analysis; D020539:Sequence Analysis, Protein; D014059:Tongue / Q000382:microbiology*; D014678:Veillonella / Q000145:classification* / Q000302:isolation & purification
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D012321:DNA-Directed RNA Polymerases; C105078:RNA polymerase beta subunit