title: Protein kinase D is a key regulator of cardiomyocyte lipoprotein lipase secretion after diabetes.
abstract: The diabetic heart switches to exclusively using fatty acid (FA) for energy supply and does so by multiple mechanisms including hydrolysis of lipoproteins by lipoprotein lipase (LPL) positioned at the vascular lumen. We determined the mechanism that leads to an increase in LPL after diabetes. Diazoxide (DZ), an agent that decreases insulin secretion and causes hyperglycemia, induced a substantial increase in LPL activity at the vascular lumen. This increase in LPL paralleled a robust phosphorylation of Hsp25, decreasing its association with PKCdelta, allowing this protein kinase to phosphorylate and activate protein kinase D (PKD), an important kinase that regulates fission of vesicles from the golgi membrane. Rottlerin, a PKCdelta inhibitor, prevented PKD phosphorylation and the subsequent increase in LPL. Incubating control myocytes with high glucose and palmitic acid (Glu+PA) also increased the phosphorylation of Hsp25, PKCdelta, and PKD in a pattern similar to that seen with diabetes, in addition to augmenting LPL activity. In myocytes in which PKD was silenced or a mutant form of PKCdelta was expressed, high Glu+PA were incapable of increasing LPL. Moreover, silencing of cardiomyocyte Hsp25 allowed phorbol 12-myristate 13-acetate to elicit a significant phosphorylation of PKCdelta, an appreciable association between PKCdelta and PKD, and a vigorous activation of PKD. As these cells also demonstrated an additional increase in LPL, our data imply that after diabetes, PKD control of LPL requires dissociation of Hsp25 from PKCdelta, association between PKCdelta and PKD, and vesicle fission. Results from this study could help in restricting cardiac LPL translocation, leading to strategies that overcome contractile dysfunction after diabetes.
authors.affiliation: Faculty of Pharmaceutical Sciences, Vancouver, BC, Canada.
authors.forename: Min Suk
authors.initials: MS
authors.lastname: Kim
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.forename: Prasanth
authors.initials: P
authors.lastname: Puthanveetil
authors.forename: Girish
authors.initials: G
authors.lastname: Kewalramani
authors.forename: Elham
authors.initials: E
authors.lastname: Hosseini-Beheshti
authors.forename: Natalie
authors.initials: N
authors.lastname: Ng
authors.forename: Yanni
authors.initials: Y
authors.lastname: Wang
authors.forename: Ujendra
authors.initials: U
authors.lastname: Kumar
authors.forename: Sheila
authors.initials: S
authors.lastname: Innis
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Proud
authors.forename: Ashraf
authors.initials: A
authors.lastname: Abrahani
authors.forename: Brian
authors.initials: B
authors.lastname: Rodrigues
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D003921:Diabetes Mellitus, Experimental / Q000201:enzymology / Q000378:metabolism*; D005947:Glucose / Q000494:pharmacology; D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins / Q000378:metabolism*; D008071:Lipoprotein Lipase / Q000032:analysis / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism*; D009363:Neoplasm Proteins / Q000378:metabolism*; D019308:Palmitic Acid / Q000494:pharmacology; D010766:Phosphorylation; D011493:Protein Kinase C / Q000378:metabolism / Q000502:physiology*; D051745:Protein Kinase C-delta / Q000378:metabolism*; D051381:Rats
chemical_list: D055551:HSP27 Heat-Shock Proteins; D006360:Heat-Shock Proteins; C486114:Hspb1 protein, rat; D009363:Neoplasm Proteins; D019308:Palmitic Acid; C089212:protein kinase D; D011493:Protein Kinase C; D051745:Protein Kinase C-delta; D008071:Lipoprotein Lipase; D005947:Glucose