title: Insulin-like growth factor-I in wound healing of rat skin.
abstract: Growth factors play an important role in orchestrating and enabling the cellular responses required for successful wound healing. In the present study, rat surgical incision was used to investigate insulin-like growth factor-I (IGF-I) expression in skin cells as well as its systemic and cutaneous tissue concentrations during acute phase of wound healing. Thirty two animals were sacrificed at days 2, 3, 5 and 9 after surgery. Eight animals were used as control. Tissue expression of IGF-I in both incisional and periincisional skin areas, as well as in skin of control unwounded animals was determined by immunohistochemistry. Serum and tissue concentrations of IGF-I were measured using RIA. Immunohistochemical analysis revealed enhanced IGF-I immunostaining in the incisional area at day 2 post-wounding. Presence of IGF-I immunoreactivity in the epidermis, as well as in dermal fibroblasts and monocytes within perivascular inflammatory infiltrate suggests its local synthesis. Although serum levels of IGF-I were not altered during wound healing, their tissue contents in the incisional area were significantly increased compared with periincisional area at days 2 and 3 after injury, as well as compared with skin content of unwounded control rats in all examined time points. Obtained results support a paracrine role of IGF-I during the acute phase of wound healing by primary intention in the rat.
authors.affiliation: Institute for Medical Research, University of Belgrade, Belgrade, Serbia. verat@imi.bg.ac.yu
authors.forename: Vera
authors.initials: V
authors.lastname: Todorović
authors.forename: Predrag
authors.initials: P
authors.lastname: Pesko
authors.forename: Marjan
authors.initials: M
authors.lastname: Micev
authors.forename: Milos
authors.initials: M
authors.lastname: Bjelović
authors.forename: Mirela
authors.initials: M
authors.lastname: Budec
authors.forename: Mileva
authors.initials: M
authors.lastname: Mićić
authors.forename: Dimitrije
authors.initials: D
authors.lastname: Brasanac
authors.forename: Olivera
authors.initials: O
authors.lastname: Ilić-Stojanović
mesh_terms: D000818:Animals; D004848:Epithelium / Q000737:chemistry / Q000293:injuries / Q000378:metabolism; D005347:Fibroblasts / Q000737:chemistry / Q000378:metabolism; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012867:Skin / Q000737:chemistry / Q000293:injuries / Q000378:metabolism*; D013997:Time Factors; D014945:Wound Healing / Q000235:genetics / Q000502:physiology*
chemical_list: D007334:Insulin-Like Growth Factor I