Large-scale production of monoclonal antibodies in suspension culture.
Monoclonal antibodies are being manufactured for clinical trials in suspension culture at the 1300-L scale. Suspension culture offers some advantages relative to high-density mammalian cell culture methods; in particular, the ability to closely monitor the behavior of cells in a homogeneous environment. Computer control and on-line mass spectrography of exit gases provide instantaneous information about the culture metabolic activity. Air sparging and agitation by marine impeller provide aeration sufficient to maintain a constant dissolved oxygen tension at cell concentrations up to 5.0 x 10(6) cells/mL without causing apparent cell damage.
Backer
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MP
Eli Lilly and Company, Lilly Research Laboratories, Indianapolis, Indiana 46285, USA.
Metzger
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LS
Slaber
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PL
Nevitt
K L
KL
Boder
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