title: Domain architecture and oligomerization properties of the paramyxovirus PIV 5 hemagglutinin-neuraminidase (HN) protein.
abstract: The mechanism by which the paramyxovirus hemagglutinin-neuraminidase (HN) protein couples receptor binding to activation of virus entry remains to be fully understood, but the HN stalk is thought to play an important role in the process. We have characterized ectodomain constructs of the parainfluenza virus 5 HN to understand better the underlying architecture and oligomerization properties that may influence HN functions. The PIV 5 neuraminidase (NA) domain is monomeric whereas the ectodomain forms a well-defined tetramer. The HN stalk also forms tetramers and higher order oligomers with high alpha-helical content. Together, the data indicate that the globular NA domains form weak intersubunit interactions at the end of the HN stalk tetramer, while stabilizing the stalk and overall oligomeric state of the ectodomain. Electron microscopy of the HN ectodomain reveals flexible arrangements of the NA and stalk domains, which may be important for understanding how these two HN domains impact virus entry.
authors.affiliation: Department of Structural Biology, Stanford University, Palo Alto, CA 94305-5126, USA.
authors.forename: Ping
authors.initials: P
authors.lastname: Yuan
authors.forename: George P
authors.initials: GP
authors.lastname: Leser
authors.forename: Borries
authors.initials: B
authors.lastname: Demeler
authors.forename: Robert A
authors.initials: RA
authors.lastname: Lamb
authors.forename: Theodore S
authors.initials: TS
authors.lastname: Jardetzky
mesh_terms: D000595:Amino Acid Sequence; D015707:HN Protein / Q000235:genetics* / Q000378:metabolism* / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D018098:Paramyxovirinae / Q000737:chemistry* / Q000502:physiology*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D025941:Protein Interaction Mapping; D017434:Protein Structure, Tertiary; D016415:Sequence Alignment; D053585:Virus Attachment; D053586:Virus Internalization
chemical_list: D015707:HN Protein