Enhanced survival of shrimp, Penaeus (Marsupenaeus) japonicus from white spot syndrome disease after oral administration of recombinant VP28 expressed in Brevibacillus brevis.
White spot syndrome virus (WSSV) disease is a major threat to shrimp culture worldwide. Here, we assessed the efficacy of the oral administration of purified recombinant VP28, an envelope protein of WSSV, expressed in a Gram-positive bacterium, Brevibacillus brevis, in providing protection in shrimp, Penaeus japonicus, upon challenge with WSSV. Juvenile shrimp (2-3g in body weight) fed with pellets containing purified recombinant VP28 (50microg/shrimp) for 2 weeks showed significantly higher survival rates than control groups when challenged with the virus at 3 days after the last day of feeding. However, when shrimp were challenged 2 weeks after the last day of feeding, survival rates decreased (33.4% and 24.93%, respectively). Survival rate was dose-dependent, increasing from 60.7 to 80.3% as the dose increased from 1 to 50microg/shrimp. At a dose of 50microg/shrimp, the recombinant protein provided protection as soon as 1 day after feeding (72.5% survival). Similar results were obtained with larger-sized shrimp. These results show that recombinant VP28 expressed in a Gram-positive bacterium is a potential oral vaccine against WSSV.
Caipang
Christopher Marlowe A
CM
Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan, Minato, Tokyo, Japan.
Verjan
Noel
N
Ooi
Ei Lin
EL
Kondo
Hidehiro
H
Hirono
Ikuo
I
Aoki
Takashi
T
Kiyono
Hiroshi
H
Yuki
Yoshikazu
Y
D000284:Administration, Oral; D000818:Animals; D033561:Penaeidae / Q000276:immunology / Q000502:physiology / Q000821:virology; D011994:Recombinant Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*; D016019:Survival Analysis; D013997:Time Factors; D019444:Vaccines, DNA / Q000276:immunology; D014764:Viral Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000276:immunology*; D014765:Viral Vaccines / Q000276:immunology*; D046848:White spot syndrome virus 1 / Q000276:immunology / Q000502:physiology*
D011994:Recombinant Proteins; D019444:Vaccines, DNA; D014764:Viral Proteins; D014765:Viral Vaccines