Synthesis and secretion of alpha-amylase by rice callus: evidence for differential gene expression.
Rice seed callus expressed and secreted alpha-amylase at high levels. Twenty percent of the protein secreted by the callus was alphaamylase. The callus secreted about 840 mug alpha-amylase with 10.9 x 10(3) units of activity per gram dry weight callus per day. The alpha-amylase from callus exhibited a more complex isoform pattern than the germinating seed alpha-amylase. In addition, the level of mRNA expression by the five alpha-amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which it attractive as a potential system for expression proteins in plant cell fermentation systems.
Simmons
C R
CR
Department of Genetics, University of California, Davis, California 95616.
Huang
N
N
Cao
Y
Y
Rodriguez
R L
RL