title: Pregnancy success of lactating Holstein cows after a single administration of a sustained-release formulation of recombinant bovine somatotropin.
abstract: BACKGROUND
  Results regarding the use of bovine somatotropin for enhancing fertility in dairy cattle are variable. Here, the hypothesis was tested that a single injection of a sustained-release preparation of bovine somatotropin (bST) during the preovulatory period would improve pregnancy success of lactating dairy cows at first service.
  
  
  RESULTS
  The first experiment was conducted in a temperate region of Mexico. Cows inseminated following natural estrus or timed artificial insemination were given a single injection of bST or a placebo injection at insemination (n = 100 cows per group). There was no significant difference between bST and control groups in the proportion of inseminated cows diagnosed pregnant (29 vs 31% pregnant). The second experiment was performed during heat stress in Florida. Cows were subjected to an ovulation synchronization regimen for first insemination. Cows treated with bST received a single injection at 3 days before insemination. Controls received no additional treatment. As expected, bST did not increase vaginal temperature. Treatment with bST did not significantly increase the proportion of inseminated cows diagnosed pregnant although it was numerically greater for the bST group (24.2% vs 17.8%, 124-132 cows per group). There was a tendency (p = 0.10) for a smaller percent of control cows to have high plasma progesterone concentrations (>/= 1 ng/ml) at Day 7 after insemination than for bST-treated cows (72.6 vs 81.1%). When only cows that were successfully synchronized were considered, the magnitude of the absolute difference in the percentage of inseminated cows that were diagnosed pregnant between bST and control cows was reduced (24.8 vs 22.4% pregnant for bST and control).
  
  
  CONCLUSIONS
  Results failed to indicate a beneficial effect of bST treatment on fertility of lactating dairy cows.
authors.affiliation: Department of Animal Sciences, University of Florida, Gainesville, Florida 32611-0910, USA. adrianeb@ufl.edu
authors.forename: A
authors.initials: A
authors.lastname: Bell
authors.forename: O A
authors.initials: OA
authors.lastname: Rodríguez
authors.forename: L A
authors.initials: LA
authors.lastname: de Castro E Paula
authors.forename: M B
authors.initials: MB
authors.lastname: Padua
authors.forename: J
authors.initials: J
authors.lastname: Hernández-Cerón
authors.forename: C G
authors.initials: CG
authors.lastname: Gutiérrez
authors.forename: A
authors.initials: A
authors.lastname: De Vries
authors.forename: P J
authors.initials: PJ
authors.lastname: Hansen
mesh_terms: D000818:Animals; D001831:Body Temperature; D002417:Cattle; D002940:Circadian Rhythm; D003692:Delayed-Action Preparations; D004334:Drug Administration Schedule; D005260:Female; D005431:Florida; D013006:Growth Hormone / Q000008:administration & dosage* / Q000737:chemistry / Q000494:pharmacology*; D006358:Hot Temperature; D007315:Insemination, Artificial / Q000662:veterinary; D008800:Mexico; D011247:Pregnancy; D011994:Recombinant Proteins / Q000008:administration & dosage* / Q000494:pharmacology*; D014621:Vagina / Q000187:drug effects
chemical_list: D003692:Delayed-Action Preparations; D011994:Recombinant Proteins; D013006:Growth Hormone