[Effect of Auricularia auricula polysaccharide on PBMCs proliferation and cytokine expression in vitro].
OBJECTIVE
To investigate the proliferation and cytokine expression of Auricularia auricula polysaccharide (AAP) on peripheral blood monnuclear cells (PBMCs) in vitro.


METHODS
AAP was obtained by water extraction and alcohol precipitation, and purified by DEAE-cellulose chromatogram. PBMCs were treated with AAP at different levels. The Cell Counting Kit-8 (CCK-8) was used to determine the proliferation of PBMCs. The concentration of TNF-alpha and TFN-gamma in supernatant were investigated by ELISA.


RESULTS
The three fractions of AAP could stimulate PBMCs proliferation at the range of 100--800 microg/ml, and AAP2 enhanced significantly the level of TNF-alpha and IFN-gamma in supernatant of PBMCs (P < 0.05, P < 0.01).


CONCLUSIONS
AAP is an immunomodulator and can enhance the immunomodulatory activity through TNF-alpha and TFN-gamma.
Zuo
Jiang-cheng
JC
The First College of Clinical Medical Science, Three Gorges University, Yichang 443002, China. jichzuo@gmail.com
Zeng
Yi-qin
YQ
He
Xiao-wen
XW
Wang
Qun-xing
QX
D001487:Basidiomycota / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D016207:Cytokines / Q000097:blood*; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007371:Interferon-gamma / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
D016207:Cytokines; D011134:Polysaccharides; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma