title: Evaluation of the human immunodeficiency virus type 1 and 2 antibodies detection in dried whole blood spots (DBS) samples.
abstract: Human Immunodeficiency Virus Type 1 and 2 antibodies detection was performed in 457 dried whole blood spots samples (S&S 903). Q-Preven HIV 1+2 was the screening test used. The results were compared with the gold standard serum tests by ELISA (Cobas Core e Axsym HIV1/2 gO) and immunofluorescence was the definitive confirmatory test. The samples were obtained from the Hospital Nossa Senhora da Conceição in Porto Alegre, RS - Brazil, through whole blood transfer to filter paper card and sent to Caxias do Sul, RS-Brazil where the tests were performed. The dried whole blood spot stability was evaluated with two different panels. The first one was composed of five negative and five positive samples stored at room temperature, 4 degrees C, -20 degrees C and -70 degrees C, while the second was composed of two negative and three positive samples stored at 37 degrees C (humidity <50%). Each sample was screened every week for six weeks. These measurement results didn't show variation during the study period. The detected sensibility was 100%, specificity was 99.6%, the positive predictive value was 99.5% and negative predictive values were 100%. The results demonstrated high performance characteristics, opening a new perspective of dried whole blood spot utilization in HIV screening diagnosis.
authors.affiliation: Programa de Pós-graduação em Patologia, Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, Rua Visconde do Herval 472, Porto Alegre, RS, Brazil. candrea@ghc.com.br
authors.forename: Andréa Cauduro de
authors.initials: AC
authors.lastname: Castro
authors.forename: Luiz Gustavo dos Anjos
authors.initials: LG
authors.lastname: Borges
authors.forename: Ricardo da Silva de
authors.initials: Rda S
authors.lastname: Souza
authors.forename: Melina
authors.initials: M
authors.lastname: Grudzinski
authors.forename: Pedro Alves
authors.initials: PA
authors.lastname: D'Azevedo
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods*; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005374:Filtration; D019084:Fluorescent Antibody Technique, Indirect; D015483:HIV Antibodies / Q000097:blood*; D015658:HIV Infections / Q000175:diagnosis* / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D007223:Infant; D008875:Middle Aged; D012680:Sensitivity and Specificity
chemical_list: D015483:HIV Antibodies