Plasmodium yoelii: novel rhoptry proteins identified within the body of merozoite rhoptries in rodent Plasmodium malaria.
The biogenesis, organization and function of the rhoptries are not well understood. Antisera were prepared to synthetic peptides prepared as multiple antigenic peptides (MAPs) obtained from a Plasmodium yoelii merozoite rhoptry proteome analysis. The antisera were used in immunofluorescence and immunoelectron microscopy of schizont-infected erythrocytes. Twenty-seven novel rhoptry proteins representing proteases, metabolic enzymes, secreted proteins and hypothetical proteins, were identified in the body of the rhoptries by immunoelectron microscopy. The merozoite rhoptries contain a heterogeneous mixture of proteins that may initiate host cell invasion and establish intracellular parasite development.
Sam-Yellowe
Tobili Y
TY
Department of Biological, Geological and Environmental Sciences, Cleveland State University, 2121 Euclid Avenue, SI 219, Cleveland OH 44115, USA. t.sam-yellowe@csuohio.edu
Banks
Tashara L
TL
Fujioka
Hisashi
H
Drazba
Judith A
JA
Yadav
Satya P
SP
D000818:Animals; D005260:Female; D019084:Fluorescent Antibody Technique, Indirect; D008288:Malaria / Q000469:parasitology*; D008297:Male; D052940:Merozoites / Q000737:chemistry / Q000648:ultrastructure; D051379:Mice; D016253:Microscopy, Immunoelectron; D015388:Organelles / Q000737:chemistry / Q000648:ultrastructure; D010967:Plasmodium yoelii / Q000737:chemistry* / Q000235:genetics / Q000276:immunology / Q000648:ultrastructure; D015800:Protozoan Proteins / Q000032:analysis* / Q000737:chemistry / Q000276:immunology; D011817:Rabbits
D015800:Protozoan Proteins