In vivo studies on the toxic effects of microcystins on mitochondrial electron transport chain and ion regulation in liver and heart of rabbit.
This study examined the toxic effects of microcystins on mitochondria of liver and heart of rabbit in vivo. Rabbits were injected i.p. with extracted microcystins (mainly MC-RR and -LR) at two doses, 12.5 and 50 MC-LReq. microg/kg bw, and the changes in mitochondria of liver and heart were studied at 1, 3, 12, 24 and 48 h after injection. MCs induced damage of mitochondrial morphology and lipid peroxidation in both liver and heart. MCs influenced respiratory activity through inhibiting NADH dehydrogenase and enhancing succinate dehydrogenase (SDH). MCs altered Na(+)-K(+)-ATPase and Ca(2+)-Mg(2+)-ATPase activities of mitochondria and consequently disrupted ionic homeostasis, which might be partly responsible for the loss of mitochondrial membrane potential (MMP). MCs were highly toxic to mitochondria with more serious damage in liver than in heart. Damage of mitochondria showed reduction at 48 h in the low dose group, suggesting that the low dose of MCs might have stimulated a compensatory response in the rabbits.
Zhao
Yanyan
Y
Fisheries College of Huazhong Agricultural University; Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Wuhan 430070, People's Republic of China.
Xie
Ping
P
Tang
Rong
R
Zhang
Xuezhen
X
Li
Li
L
Li
Dapeng
D
D000818:Animals; D004579:Electron Transport / Q000187:drug effects*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D052998:Microcystins / Q000633:toxicity*; D008929:Mitochondria, Heart / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D008930:Mitochondria, Liver / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D011817:Rabbits; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism
D052998:Microcystins; D008315:Malondialdehyde; D000254:Sodium-Potassium-Exchanging ATPase