Frequency modulation of synchronized Ca(2+) spikes in cultured hippocampal networks through mTOR.
The atypical serine/threonine protein kinase, a mammalian target of rapamycin (mTOR), is believed to be essential to the regulation of cell growth and the functions of the central nervous system. By using calcium imaging and patch-clamping techniques to study the role of this signaling pathway in the activity of cultured hippocampal neurons, we found that rapamycin significantly reduces the spontaneous activities of network neurons as well as the efficacy of synaptic transmission through insulin-mTOR signaling pathway. Our study sheds light on understanding the role of mTOR signaling pathway in controlling the information processing of network neurons.
Li
Ru-Xin
RX
Department of Biological Sciences and Biotechnology, National State Key Laboratory of Biomembrane and Membrane Biotechnology, Tsinghua University, Beijing, China.
Yu
Xin-Zhu
XZ
Fu
Min
M
Zong
Min
M
Liu
Ru-Lin
RL
Xie
Zuo-Ping
ZP
D000200:Action Potentials / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects* / Q000502:physiology; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation; D004622:Embryo, Mammalian; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D006624:Hippocampus / Q000166:cytology*; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018408:Patch-Clamp Techniques; D051381:Rats; D020123:Sirolimus / Q000494:pharmacology*; D013997:Time Factors
D004791:Enzyme Inhibitors; D002118:Calcium; D020123:Sirolimus