[Biocompatibility of polyurethane-BaFe(12)O(19 ) composite microsphere as a new endovascular embolization material].
OBJECTIVE
To investigate the biocompatibility of polyurethane-BaFe(12)O(19) magnetic composite microsphere as a new endovascular embolization material.


METHODS
The biocompatibility of BaFe(12)O(19) particle was evaluated in vitro using Ames test, cell toxicity test, acute and subacute systemic toxicity test, hemolysis test, bleeding time and clotting time test and blood clotting function assay.


RESULTS
Ames test showed that the MR values of this particle leaching solution were all less than 2 without mutagenicity. Cell toxicity test showed that leaching solution at different concentrations had grade I toxicity on L929 cells. Acute and subacute systemic toxicity test showed that the experimental animals had good general condition without obvious pathological abnormality. The hemolysis rate of experimental group was 2.43%, which met the ISO standard (no more than 5%). The bleeding time and clotting time in mice were comparable between the experimental group and control group (P>0.05). There were no significant differences in blood clotting function between experimental group and control group (P>0.05).


CONCLUSIONS
The material has no obvious toxicity or mutagenicity, and does not cause hemolysis or hemopexis or affect the bleeding time and clotting time. Polyurethane-BaFe(12)O( 19) particle possesses satisfactory biocompatibility.
Guo
Zai-Yu
ZY
Department of Neurosurgery, Wuhan General Hospital of Guangzhou Military Command, Wuhan 430070, China. guozai_yu@163.com
Ma
Lian-Ting
LT
Li
Jun
J
Qin
Hai-Lin
HL
DU
Hao
H
Dai
Hong-Lian
HL
D000818:Animals; D017609:Barium Compounds / Q000737:chemistry* / Q000633:toxicity; D001672:Biocompatible Materials; D045744:Cell Line, Tumor; D004621:Embolization, Therapeutic / Q000295:instrumentation*; D005290:Ferric Compounds / Q000737:chemistry* / Q000633:toxicity; D008863:Microspheres; D011140:Polyurethanes / Q000737:chemistry* / Q000633:toxicity; D018675:Toxicity Tests
D017609:Barium Compounds; D001672:Biocompatible Materials; D005290:Ferric Compounds; D011140:Polyurethanes; C016258:barium ferrite