Identification of a rare subset of adipose tissue-resident progenitor cells, which express CD133 and TRPC3 as a VEGF-regulated Ca2+ entry channel.
VEGF-induced Ca2+ signalling was investigated in CD133+/VEGFR-2+ progenitor cells isolated from human adipose stroma. Colonies derived from CD133+ immunoselected cells displayed inhomogenous Ca2+ signals, with variable magnitude of VEGF-induced Ca2+ entry, which positively correlated with expression of the Ca2+ channel protein TRPC3. High levels of VEGF-induced Ca2+ entry and TRPC3 expression were preferentially detected in rim areas of expanding colonies. Dominant negative suppression of TRPC3 inhibited VEGF-induced Ca2+ entry into CD133+ cells. Our results identify TRPC3 as a key Ca2+ entry channel in a subset of CD133+ stem cells. We suggest TRPC3 as an essential determinant of cell fate in CD133+ progenitor-derived colonies.
Poteser
Michael
M
Institute of Pharmaceutical Sciences, Pharmacology and Toxicology, Karl-Franzens-University of Graz, Graz, Austria.
Graziani
Annarita
A
Eder
Petra
P
Yates
Ameli
A
Mächler
Heinrich
H
Romanin
Christoph
C
Groschner
Klaus
K
D000071916:AC133 Antigen; D000273:Adipose Tissue / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000096:biosynthesis*; D015220:Calcium Channels / Q000096:biosynthesis* / Q000235:genetics; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D005260:Female; D006023:Glycoproteins / Q000096:biosynthesis*; D006801:Humans; D008297:Male; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D010455:Peptides; D013234:Stem Cells / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D050052:TRPC Cation Channels / Q000096:biosynthesis* / Q000235:genetics; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism* / Q000494:pharmacology
D000071916:AC133 Antigen; D015703:Antigens, CD; D015220:Calcium Channels; D006023:Glycoproteins; C000606023:PROM1 protein, human; D010455:Peptides; D050052:TRPC Cation Channels; C104603:TRPC3 cation channel; D042461:Vascular Endothelial Growth Factor A