title: Plasma insulin concentrations during a hyperinsulinaemic clamp: what do we measure?
abstract: BACKGROUND
  The aim of this study is to compare the performance of three commonly used insulin assays with respect to the detection of exogenous human and porcine insulin added to human or rat plasma.
  
  
  METHODS
  The DPC Immulite human insulin assay, the Mercodia rat insulin enzyme-linked immunosorbent assay and the Linco rat insulin radioimmunoassay were tested.
  
  
  RESULTS
  The mean cross-reactivity of exogenous insulin ranged from 25% to 92%. The mean cross-reactivity of Actrapid in human plasma on the DPC Immulite was 56% and was independent of the endogenous insulin concentration.
  
  
  CONCLUSIONS
  The measurement of exogenous insulin varies according to the source of exogenous insulin, matrix and insulin assay.
authors.affiliation: Laboratory of Endocrinology, Department of Clinical Chemistry, University of Amsterdam. m.t.ackermans@amc.uva.nl
authors.forename: M T
authors.initials: MT
authors.lastname: Ackermans
authors.forename: L P
authors.initials: LP
authors.lastname: Klieverik
authors.forename: E
authors.initials: E
authors.lastname: Endert
authors.forename: H P
authors.initials: HP
authors.lastname: Sauerwein
authors.forename: A
authors.initials: A
authors.lastname: Kalsbeek
authors.forename: E
authors.initials: E
authors.lastname: Fliers
mesh_terms: D000818:Animals; D003429:Cross Reactions; D015309:Glucose Clamp Technique / Q000379:methods*; D006801:Humans; D007328:Insulin / Q000097:blood* / Q000276:immunology*; D051381:Rats; D013552:Swine
chemical_list: D007328:Insulin