title: Orthogonal electrophoretic fractionation of DNA in agarose gels.
abstract: We developed an electrophoretic procedure, using Voltage Gradient Gel Electrophoresis (VGGE), which allows to obtain both an improvement of the resolution power of the system in orthogonal fractionation of DNA and, mainly, an about fourfold enhancement of hybridization signals in Southern blotting applications.
authors.affiliation: Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, V.le delle Scienze, Edificio 16, Palermo, Italy.
authors.forename: Daniele
authors.initials: D
authors.lastname: Bellavia
authors.forename: Giorgia
authors.initials: G
authors.lastname: Sisino
authors.forename: Giada
authors.initials: G
authors.lastname: Sisino
authors.forename: Rainer
authors.initials: R
authors.lastname: Barbieri
mesh_terms: D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D016753:Interleukin-10 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
chemical_list: D012333:RNA, Messenger; D016753:Interleukin-10; D004247:DNA