Effects of hyperbaric oxygen therapy on tumor growth in murine model of PC-3 prostate cancer cell line.
OBJECTIVE
To test the hypothesis that hyperbaric oxygen (HBO) has no effect on tumor growth in a murine model of indolent in vivo prostate cancer. HBO means breathing pure (100%) oxygen under increased atmospheric pressure.


METHODS
Human prostate PC-3 cells were injected into 40 severe combined-immunodeficient mice. They were randomized to undergo 20 sessions of either HBO or normobaric air in standardized conditions and observed for 4 weeks before histologic assessment of any palpable tumors that had developed. The analysis of the developed PC-3 tumors included tumor volume, microvessel density, apoptosis-associated markers (ie, p53, p27), and the proliferative index (Ki-67).


RESULTS
The exposure to HBO at 2 atm for 20 treatment sessions, which comprised a daily 90-minute session, 5 d/wk, had no effect on the prostate cancer volume (P > .05). No differences were observed in tumor microvessel density, proliferative index, or apoptosis markers compared with the non-HBO group (P > .05).


CONCLUSIONS
HBO did not have a tumor stimulatory effect on prostate cancer and could potentially be used safely in conjunction with other therapeutic modalities.
Tang
Hao
H
Department of Urology, Shanghai Changhai Hospital, Shanghai, China.
Sun
Yinghao
Y
Xu
Chuanliang
C
Zhou
Tie
T
Gao
Xu
X
Wang
Linhui
L
D000818:Animals; D045744:Cell Line, Tumor; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation; D008297:Male; D051379:Mice; D011471:Prostatic Neoplasms / Q000473:pathology*