Isolation, purification and antitumor activity of lipopolysaccharide from cow placenta.
Lipopolysaccharide from cow placenta (LPS-CP-2) has been isolated and purified by hot phenol-water extraction, enzyme hydrolysis, chloroform-petroleum ether method, ion-exchange and gel-filtration chromatography. Also, LPS-PS-2 was evaluated for antitumor activity against Ehrlich ascites carcinoma (EAC)-bearing Swiss albino mice. LPS-PS-2 caused significant (P<0.05) decrease in tumor volume, and viable cell count; and it prolonged the life span of EAC-tumor-bearing mice. Hematological profile indicates that LPS-CP-2 possessed protective action on the haemopoietic system. Further, administration of LPS-CP-2 reduced the tumor volume of both DLA and EAC cell lines in a dose-dependent way. The LPS-PS was found to be devoid of pyrogenic response in the rabbits. These results indicate that LPS-PS exhibited significant antitumor activity without pyrogenic response, suggesting its potential as antitumor agent.
Li
Haiping
H
Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, PR China. hhppli@163.com
Lu
Xiaoxiang
X
Lu
Meijun
M
Liu
Hongmei
H
D000818:Animals; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D002286:Carcinoma, Ehrlich Tumor / Q000097:blood / Q000188:drug therapy* / Q000473:pathology; D002417:Cattle; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005260:Female; D008070:Lipopolysaccharides / Q000302:isolation & purification* / Q000494:pharmacology* / Q000627:therapeutic use; D051379:Mice; D010920:Placenta / Q000737:chemistry*; D011247:Pregnancy; D011749:Pyrogens / Q000378:metabolism; D011817:Rabbits
D000970:Antineoplastic Agents; D008070:Lipopolysaccharides; D011749:Pyrogens