title: Occurrence of residues of the veterinary drug malachite green in eels caught downstream from municipal sewage treatment plants.
abstract: Residues of malachite green (MG), a veterinary drug illegally used for the treatment of aquacultured fish, have been found in wild eels caught from surface waters downstream from the sewers of different municipal sewage treatment plants (STPs). MG and its metabolite leucomalachite green (LMG) were detected with total concentrations up to 0.765 microg kg(-1) fresh weight in the tissues of 25 out of 45 eels caught from different lakes, a river and a canal in Berlin, Germany. In all cases, the occurrence of the residues could directly be linked to the presence of discharges by municipal STPs into the receiving surface waters. MG is a multiple-use compound that is also used to color materials. Thus, it appears to be reasonable that the residues of MG found in the eel samples originate from such uses, e.g. by wash off from clothes or paper towels colored with MG. Additional loads from legal uses of MG as veterinary drug for the treatment of ornamental fish (private aquaria) are possible. The results obtained from this study are the first proof of background contaminations of a veterinary drug found in samples of fish not intentionally treated with such agents. MG and LMG are regarded as potential genotoxic carcinogens making it impossible to establish a tolerable daily intake. A margin of exposure (MOE) approach was applied to evaluate the human health risk associated with the consumption of the eels investigated in this study. With a MOE of 3.4 million the chronic risk was classified as being very low. Nevertheless, due to their potential to act as genotoxic carcinogens, any oral exposure to residues of MG and LMG should be avoided. According to European Union law, zero tolerance applies to all residues of MG and LMG found in food for human consumption as MG is not registered for use as veterinary drug.
authors.affiliation: Federal Institute for Risk Assessment (BfR), Section for Residues of Medicinal Products, Diedersdorfer Weg 1, 12277 Berlin, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Schuetze
authors.forename: Thomas
authors.initials: T
authors.lastname: Heberer
authors.forename: Susanne
authors.initials: S
authors.lastname: Juergensen
mesh_terms: D000818:Animals; D001604:Berlin; D004524:Eels; D005843:Geography; D005858:Germany; D015394:Molecular Structure; D012394:Rosaniline Dyes / Q000032:analysis* / Q000737:chemistry; D012722:Sewage / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis* / Q000737:chemistry
chemical_list: D012394:Rosaniline Dyes; D012722:Sewage; D014874:Water Pollutants, Chemical; C005095:malachite green