title: Hepatic monooxygenase (CYP1A and CYP3A) and UDPGT enzymatic activities as biomarkers for long-term carbofuran exposure in tench (Tinca tinca L).
abstract: The effect of a long-term exposure of tenchs to different concentrations (10 and 100 micro g/L) of the pesticide carbofuran has been evaluated. Microsomal hepatic cytochrome P450 subfamily 1A (CYP1A) and 3A (CYP3A) activities, as well as the phase II enzyme uridine diphospho-glucuronosyltransferase (UDPGT) activity were evaluated as adequate biomarkers of fish exposure to environmentally relevant concentrations of the pesticide carbofuran in freshwater ecosystems. A clear time-dependent inhibition of both CYP1A and UDPGT activities was observed in fish exposed to the highest dose of carbofuran with respect to controls, whereas in the case of CYP3A activity, values of exposed animals did not show a clear pattern of alteration during the experiment. The results of the present study demonstrated that hepatic CYP1A and UDPGT activities from tench could be considered as sensitive biomarkers for carbamate pesticides in polluted water, thus allowing future and ecologically relevant biomonitoring studies with this species.
authors.affiliation: Toxicology Area, Faculty of Veterinary Medicine, Avda de la Universidad, Caceres, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Hernández-Moreno
authors.forename: Francisco
authors.initials: F
authors.lastname: Soler-Rodríguez
authors.forename: M Prado
authors.initials: MP
authors.lastname: Míguez-Santiyán
authors.forename: Marcos
authors.initials: M
authors.lastname: Pérez-López
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002235:Carbofuran / Q000378:metabolism / Q000633:toxicity; D003530:Cyprinidae / Q000378:metabolism*; D019363:Cytochrome P-450 CYP1A1 / Q000378:metabolism*; D051544:Cytochrome P-450 CYP3A / Q000378:metabolism*; D004789:Enzyme Activation; D014453:Glucuronosyltransferase / Q000378:metabolism*; D008862:Microsomes, Liver / Q000201:enzymology*; D010105:Oxygenases / Q000378:metabolism; D010575:Pesticides / Q000378:metabolism / Q000633:toxicity; D013997:Time Factors; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity; D000099148:UGT1A1 Enzyme
chemical_list: D015415:Biomarkers; D002235:Carbofuran; D019363:Cytochrome P-450 CYP1A1; D051544:Cytochrome P-450 CYP3A; D014453:Glucuronosyltransferase; D010105:Oxygenases; D010575:Pesticides; D014874:Water Pollutants, Chemical; D000099148:UGT1A1 Enzyme; C020066:dimethylaniline monooxygenase (N-oxide forming)