Discovery of altered protein profiles in epithelial ovarian carcinogenesis by SELDI mass spectrometry.
OBJECTIVE
Identification of proteomic alterations in epithelial ovarian tumorigenesis may facilitate the understanding of progression of this disease.


METHODS
Specific protein peak patterns were identified in 20 microdissected epithelial ovarian tumors (13 epithelial ovarian cancers (EOCs) and 7 low malignant potential (LMP) tumors), as well as in the matched normal cells. Protein profiles were generated by surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) from all the different types of cells.


RESULTS
Among seven protein peaks from EOC cells, six were significantly increased while one was decreased compared with normal cells, and three peaks from LMP cells were markedly increased while one was decreased compared with normal cells.


CONCLUSIONS
The combination of SELDI and laser capture microdissection (LCM) is effective in finding the key molecules in ovarian tumorigenesis. Further identification of these protein peaks is important and these malignant protein signatures lend themselves to identification of populations at high-risk for EOC and for monitoring response to EOC chemopreventive agents.
Luo
J
J
Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Qian
J H
JH
Yu
J K
JK
Zheng
S
S
Xie
X
X
Lu
W G
WG
D004848:Epithelium / Q000378:metabolism; D005260:Female; D006801:Humans; D042282:Microdissection; D010051:Ovarian Neoplasms / Q000378:metabolism* / Q000473:pathology; D010053:Ovary / Q000737:chemistry / Q000473:pathology; D040081:Protein Array Analysis / Q000379:methods*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*