title: Gender-specific effect of estrogen receptor-1 gene polymorphisms in coronary artery disease and its angiographic severity in Chinese population.
abstract: BACKGROUND
  The role of common polymorphisms of the estrogen receptor-1 in coronary artery disease (CAD) and it association with angiographic severity reminds conflicting in sexes and different races.
  
  
  METHODS
  Two-hundred ten angiographically defined Chinese CAD patients and 174 control subjects were enrolled. DNA was obtained and the polymorphisms were analyzed by the polymerase chain reaction. The region containing the PvuII T/C and the XbaI A/G sites was amplified. PCR product was cleaved with the restriction endonucleases.
  
  
  RESULTS
  No significant differences in PvuII and XbaI genotype and allele frequencies were noted between the CAD and controls.However, when stratified by gender, we noticed the PvuII genotype and allele frequencies were significantly different between CAD and controls, but in male group only, not in female group. Diabetes, hypertension, high LDL levels and the PvuII CC genotype were independent risk factors for CAD. PvuII CC was associated with the angiographic severity of CAD measuring by the number of diseased vessels as well. For XbaI, no association was found with the CAD susceptibility before and after gender stratification.
  
  
  CONCLUSIONS
  This study revealed a gender-specific effect of PvuII polymorphism in Chinese CAD subjects. PvuII gene polymorphisms affect CAD susceptibility in man only. The PvuII CC is a risk factor for CAD and it is associated with angiographic CAD severity.
authors.affiliation: Department of Cardiology, Hangzhou First Municipal Hospital and Hangzhou Hospital, Nanjing Medical University, Hangzhou 310006, China.
authors.forename: Haiying
authors.initials: H
authors.lastname: Xu
authors.forename: Xuwei
authors.initials: X
authors.lastname: Hou
authors.forename: Ningfu
authors.initials: N
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Hui
authors.forename: Jianfen
authors.initials: J
authors.lastname: Jin
authors.forename: Shen
authors.initials: S
authors.lastname: Yun
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.forename: Xiuying
authors.initials: X
authors.lastname: He
authors.forename: Jianjun
authors.initials: J
authors.lastname: He
authors.forename: Sheng
authors.initials: S
authors.lastname: Zhang
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Han
mesh_terms: D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002681:China / Q000453:epidemiology; D017023:Coronary Angiography; D003324:Coronary Artery Disease / Q000000981:diagnostic imaging* / Q000453:epidemiology / Q000235:genetics*; D004247:DNA / Q000235:genetics; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012720:Severity of Illness Index; D012737:Sex Factors
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; D004247:DNA