[Effects of visfatin gene overexpression on insulin sensitivity in the insulin-resistant rats induced by high-fat diet].
OBJECTIVE
To investigate the effects of visfatin gene overexpression on insulin sensitivity in insulin-resistant (IR) rats induced by high-fat diet.


METHODS
The recombinant visfatin plasmid was constructed and transfected into IR rats induced by high-fat diet. The euglycemic-hyperinsulinemic clamp experiments were performed for evaluation the change of insulin sensitivity before and after administration.


RESULTS
The expression plasmid of visfatin were successfully constructed. After 3 days for plasmid injecting, plasma visfatin levels and glucose infusion rates were significantly increased (2.19 +/- 0.36 vs 0.98 +/- 0.27 and 32.6 +/- 1.2 vs 24.0 +/- 1.2 mg x kg(-1) x min(-1), respectively, all P < 0.01), and total cholesterol and high-density lipoprotein cholesterol (HDL-C) were significantly decreased (2.36 +/- 0.22 vs 1.60 +/- 0.21 mmol/L and 1.41 +/- 0.24 vs 0.88 +/- 0.11 mmol/L, respectively, all P < 0.05) in high-fat diet rats.


CONCLUSIONS
The transfection of visfatin plasmid enhanced plasma visfatin level and improved insulin sensitivity in IR rats induced by high-fat diet.
Qin
Sun
S
Department of Endocrinology, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
Li
Ling
L
Yang
Gang-Yi
GY
Li
Ren-Zhe
RZ
Li
Ke
K
Qi
Xiao-Ya
XY
Li
Sheng-Bing
SB
Chen
Wen-Wen
WW
Liu
Hua
H
Tang
Yi
Y
Boden
Guenther
G
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D004041:Dietary Fats / Q000633:toxicity; D004351:Drug Resistance / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D007333:Insulin Resistance; D008297:Male; D054409:Nicotinamide Phosphoribosyltransferase / Q000097:blood* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
D001786:Blood Glucose; D004041:Dietary Fats; D007004:Hypoglycemic Agents; D007328:Insulin; D054409:Nicotinamide Phosphoribosyltransferase