title: [RP-HPLC determination of betulinic acid in Callicarpa macrophylla].
abstract: OBJECTIVE
  To establish an RP-HPLC method for determination of betulinic acid in Callicarpa macrophylla, a commonly used herbal in Yunnan.
  
  
  METHODS
  A Kromasil-C18 column (4.6 mm x 200 mm, 5 microm) and a mobile phase consisted of acetonitrile-0.1% phosphoric acid (63: 37) were used. The flow rate was 1.0 mL x min(-1) and the UV detector wavelength was 205 nm.
  
  
  RESULTS
  Betulinic acid was well separated from other compounds in C. macrophylla. The content of betulinic acid in C. macrophylla from different origins showed apparent differences, the content of betulinic acid in C. macrophylla from Yunnan was the highest. The calibration curve was linear in the range of 0.016 6-0.332 mg x mL(-1) of betulinic acid with correlation coefficient 0.999 8. The average recovery of betulinic acid was 98.5%.
  
  
  CONCLUSIONS
  The method is simple, accurate and reproducible, and is suitable for the quality control of C. macrophylla.
authors.affiliation: School of Chinese Materia Medicia, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Pan
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Jia
authors.forename: Qi-Shi
authors.initials: QS
authors.lastname: Sun
mesh_terms: D002138:Calibration; D032408:Callicarpa / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D016014:Linear Models; D053978:Pentacyclic Triterpenes; D011786:Quality Control; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014315:Triterpenes / Q000032:analysis* / Q000302:isolation & purification; D000094062:Betulinic Acid
chemical_list: D004365:Drugs, Chinese Herbal; D053978:Pentacyclic Triterpenes; D014315:Triterpenes; D000094062:Betulinic Acid