title: Molecular cloning and characterization of the Dicer-like 2 gene from Brassica rapa.
abstract: Dicer-like proteins (DCLs) are involved in small RNA-mediated development and viral defense in plants. In model plants, at least four DCLs have been found and a number of studies have helped to understand their function. However, the function of the Dicer or DCLs in other plants is still unclear. Here, we report the full-length cDNA sequence of Brassica rapa ssp. chinensis DCL2 (BrDCL2) gene, which contains a 4,179 bp open reading frame (ORF) encoding a protein of 1,392 amino acids. At the 3' end of BrDCL2, clones with three different lengths of 3' untranslated region were found. An alternative splice variant of BrDCL2, BrDCL2sv, in which one intron was retained between exon9 and exon10, was also cloned. Because of a change in the coding sequence resulting in a premature terminal codon, BrDCL2sv was expected to translate a short peptide containing the whole DEXHc domain.
authors.affiliation: Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.forename: Jiejun
authors.initials: J
authors.lastname: Peng
authors.forename: Yuwen
authors.initials: Y
authors.lastname: Lu
authors.forename: Lin
authors.initials: L
authors.lastname: Lin
authors.forename: Hongying
authors.initials: H
authors.lastname: Zheng
authors.forename: Hairu
authors.initials: H
authors.lastname: Chen
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Adams
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D029741:Brassica rapa / Q000201:enzymology* / Q000235:genetics; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D006801:Humans; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010940:Plant Proteins / Q000235:genetics; D020033:Protein Isoforms; D043244:Ribonuclease III / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D018076:DNA, Complementary; D010940:Plant Proteins; D020033:Protein Isoforms; D043244:Ribonuclease III