title: Proteomic insights into Helicobacter pylori coccoid forms under oxidative stress.
abstract: Helicobacter pylori, an etiological agent of gastroduodenal diseases, undergoes drastic morphological transition from spiral shape to coccoid form under oxidative stress. However, the knowledge of the specific expression profile in response to oxidative stress is relatively limited. Here, we report global proteomic analysis of H. pylori coccoids under oxidative stress. Two-dimensional gel electrophoresis analysis of H. pylori featuring coccoid revealed that 10 unique protein spots exhibit different expression profiles with comparison of that under normal microaerophilic condition. In total, seven proteins including superoxide dismutase, alkyl hydroperoxide reductase, urease G, and so forth were confirmed using matrix-assisted laser desorption/ionization time-of-flight/mass spectroscopy and then validated by reverse transcription-polymerase chain reaction, indicating that they play key roles in the physiological adaptation mechanisms of H. pylori to oxygen challenge. These data provide preliminary insights into H. pylori on coccoid generation under oxidative stress.
authors.affiliation: Department of Clinical Microbiology and Immunology, Faculty of Medical Laboratory Science, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zeng
authors.forename: Gang
authors.initials: G
authors.lastname: Guo
authors.forename: Xu Hu
authors.initials: XH
authors.lastname: Mao
authors.forename: Wen De
authors.initials: WD
authors.lastname: Tong
authors.forename: Quan Ming
authors.initials: QM
authors.lastname: Zou
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D015964:Gene Expression Regulation, Bacterial; D018869:Heat-Shock Response; D016480:Helicobacter pylori / Q000235:genetics / Q000378:metabolism / Q000502:physiology* / Q000648:ultrastructure*; D006801:Humans; D046529:Microscopy, Electron, Transmission; D018384:Oxidative Stress; D020543:Proteome; D040901:Proteomics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D001426:Bacterial Proteins; D020543:Proteome