title: Determination of pyruvic acid by using enzymic fluorescence capillary analysis.
abstract: A new method (P-LE-FCA) for the determination of pyruvic acid was proposed based on liquid enzyme method (LE) and fluorescence capillary analysis (FCA). The optimum experimental conditions were as follows: the excitation and emission wavelengths were 350 and 460 nm, respectively; the reaction time and temperature were 20 min and 38 degrees C, respectively; the pH of phosphate buffer solution was 7.5; the concentrations of nicotinamide adenine dinucleotide and lactate dehydrogenase were 1.0 mmol L(-1) and 5.0 k UL(-1), respectively. The linear range of this method was 0.2-1.2 mmol L(-1) (Delta F=327.13C-10.018, r=0.9942). Its detection limit was 0.012 mmol L(-1). And its relative standard deviation was 0.86%. Only 18 microL of total reaction solution is enough for the detection. P-LE-FCA has some merits such as lower cost, simple operation procedure and micro determination. It has been used for the determination of pyruvic acid content in human urine samples.
authors.affiliation: School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Zhao
authors.forename: Xiu-Feng
authors.initials: XF
authors.lastname: Gao
authors.forename: Yong-Sheng
authors.initials: YS
authors.lastname: Li
authors.forename: Xiang
authors.initials: X
authors.lastname: Ju
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Zheng
mesh_terms: D005470:Fluorometry / Q000379:methods*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008832:Microchemistry; D009243:NAD / Q000378:metabolism; D019289:Pyruvic Acid / Q000652:urine*
chemical_list: D009243:NAD; D019289:Pyruvic Acid; D007770:L-Lactate Dehydrogenase