Co-factor engineering in lactobacilli: effects of uncoupled ATPase activity on metabolic fluxes in Lactobacillus (L.) plantarum and L. sakei.
The hydrolytic F(1)-part of the F(1)F(0)-ATPase was over-expressed in Lactobacillus (L.) plantarum NC8 and L. sakei Lb790x during fermentation of glucose or ribose, in order to study how changes in the intracellular levels of ATP and ADP affect the metabolic fluxes. The uncoupled ATPase activity resulted in a decrease in intracellular energy level (ATP/ADP ratio), biomass yield and growth rate. Interestingly, the glycolytic and ribolytic flux increased in L. plantarum with uncoupled ATPase activity compared to the reference strain by up to 20% and 50%, respectively. The ATP demand was estimated to have approximately 80% control on both the glycolytic and ribolytic flux in L. plantarum under these conditions. In contrast, the glycolytic and ribolytic flux decreased in L. sakei with uncoupled ATPase activity.
Rud
Ida
I
Matforsk, Nofima Food, Osloveien 1, N-1430 As, Norway; Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 As, Norway.
Solem
Christian
C
Jensen
Peter Ruhdal
PR
Axelsson
Lars
L
Naterstad
Kristine
K
D000244:Adenosine Diphosphate / Q000235:genetics / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D004734:Energy Metabolism / Q000502:physiology*; D005285:Fermentation / Q000502:physiology; D005947:Glucose / Q000235:genetics / Q000378:metabolism; D048191:Lactiplantibacillus plantarum / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D006180:Proton-Translocating ATPases / Q000235:genetics / Q000378:metabolism*; D012266:Ribose / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D000244:Adenosine Diphosphate; D012266:Ribose; D000255:Adenosine Triphosphate; D006180:Proton-Translocating ATPases; D005947:Glucose