title: An activity-regulated microRNA controls dendritic plasticity by down-regulating p250GAP.
abstract: Activity-regulated gene expression is believed to play a key role in the development and refinement of neuronal circuitry. Nevertheless, the transcriptional networks that regulate synapse growth and plasticity remain largely uncharacterized. Here, we show that microRNA 132 (miR132) is an activity-dependent rapid response gene regulated by the cAMP response element-binding (CREB) protein pathway. Introduction of miR132 into hippocampal neurons enhanced dendrite morphogenesis whereas inhibition of miR132 by 2'O-methyl RNA antagonists blocked these effects. Furthermore, neuronal activity inhibited translation of p250GAP, a miR132 target, and siRNA-mediated knockdown of p250GAP mimicked miR132-induced dendrite growth. Experiments using dominant-interfering mutants suggested that Rac signaling is downstream of miR132 and p250GAP. We propose that the miR132-p250GAP pathway plays a key role in activity-dependent structural and functional plasticity.
authors.affiliation: Department of Veterinary Anatomy, Physiology, and Pharmacology, Washington State University, 100 Dairy Road, Pullman, WA 99164, USA. waymang@ohsu.edu
authors.forename: Gary A
authors.initials: GA
authors.lastname: Wayman
authors.forename: Monika
authors.initials: M
authors.lastname: Davare
authors.forename: Hideaki
authors.initials: H
authors.lastname: Ando
authors.forename: Dale
authors.initials: D
authors.lastname: Fortin
authors.forename: Olga
authors.initials: O
authors.lastname: Varlamova
authors.forename: Hai-Ying M
authors.initials: HY
authors.lastname: Cheng
authors.forename: Daniel
authors.initials: D
authors.lastname: Marks
authors.forename: Karl
authors.initials: K
authors.lastname: Obrietan
authors.forename: Thomas R
authors.initials: TR
authors.lastname: Soderling
authors.forename: Richard H
authors.initials: RH
authors.lastname: Goodman
authors.forename: Soren
authors.initials: S
authors.lastname: Impey
mesh_terms: D000818:Animals; D017362:Cyclic AMP Response Element-Binding Protein / Q000378:metabolism; D003712:Dendrites / Q000378:metabolism*; D015536:Down-Regulation / Q000235:genetics*; D020690:GTPase-Activating Proteins / Q000235:genetics*; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009473:Neuronal Plasticity; D014176:Protein Biosynthesis; D051381:Rats; D017207:Rats, Sprague-Dawley; D009435:Synaptic Transmission
chemical_list: C470196:ARHGAP32 protein, human; D017362:Cyclic AMP Response Element-Binding Protein; D020690:GTPase-Activating Proteins; C547077:MIRN132 microRNA, human; D035683:MicroRNAs