title: Toward improved biochips based on rolling circle amplification--influences of the microenvironment on the fluorescence properties of labeled DNA oligonucleotides.
abstract: Microarrays have become an increasingly important tool for biotechnology and molecular diagnostics. Despite many advantages, their sensitivity is still insufficient for such tasks as the analysis of small sample quantities and for the detection of alterations in gene expression of low-abundance genes. Accordingly, amplification strategies are necessary. Approaches to amplify the signal intensity include the increase of the number of dye molecules per target through either particle labels or rolling circle amplification, as used for this study.
authors.affiliation: Federal Institute for Materials Research and Testing (BAM), I.5 Bioanalytics, Richard-Willstätter-Str. 11, 12489 Berlin, Germany. elke.mayer-enthart@bam.de
authors.forename: Elke
authors.initials: E
authors.lastname: Mayer-Enthart
authors.forename: Julien
authors.initials: J
authors.lastname: Sialelli
authors.forename: Knut
authors.initials: K
authors.lastname: Rurack
authors.forename: Ute
authors.initials: U
authors.lastname: Resch-Genger
authors.forename: Daniela
authors.initials: D
authors.lastname: Köster
authors.forename: Harald
authors.initials: H
authors.lastname: Seitz
mesh_terms: D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D011088:DNA Ligases; D004270:DNA, Circular / Q000737:chemistry*; D004277:DNA, Single-Stranded; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000494:pharmacology*; D008956:Models, Chemical; D008957:Models, Genetic; D021141:Nucleic Acid Amplification Techniques; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*
chemical_list: D002232:Carbocyanines; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; C089187:cyanine dye 3; D004247:DNA; D011088:DNA Ligases