title: Morphological and functional characterization of BcMF13 in the antisense-silenced plants of Brassica campestris ssp. chinensis var. parachinensis.
abstract: The gene Brassica campestris male fertility 13 (BcMF13, GenBank accession number EF158459) was isolated as a reproductive organ-specific gene from Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis). It is exclusively expressed in stage four and five flower buds of fertile lines and is most strongly expressed in stamens. Here, we report a functional characterization of this BcMF13 gene in the antisense-silenced plants. The inflorescence of the BcMF13 mutant was compacted with anthers curved outside. The fertility of this mutant was greatly reduced with less than 5 seeds per silique. Under scanning electron microscopy, the mutant demonstrated numerous shriveled pollen grains with deep invaginations. The frequency of normal pollen grains was just 45.34%. The pollen mother cell, the tetrad, and the mature pollen of the BcMF13 mutant were abnormal resulting in the poor pollen vitality. Germination test in vivo suggested BcMF13 delayed the pollen tubes' extension in the style. All these indicated BcMF13 had a vital role in pollen development of Chinese cabbage.
authors.affiliation: Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Li
authors.forename: Jiashu
authors.initials: J
authors.lastname: Cao
mesh_terms: D001937:Brassica / Q000033:anatomy & histology* / Q000166:cytology / Q000378:metabolism* / Q000648:ultrastructure; D020868:Gene Silencing; D018525:Germination; D009154:Mutation / Q000235:genetics; D010940:Plant Proteins / Q000378:metabolism*; D011058:Pollen / Q000166:cytology / Q000648:ultrastructure; D016372:RNA, Antisense / Q000378:metabolism*; D013194:Staining and Labeling
chemical_list: D010940:Plant Proteins; D016372:RNA, Antisense