[Relationship between anti-myelin basic protein antibody and myelinoclasis in rat brain stem after brain trauma].
OBJECTIVE
To investigate the relations between anti-myelin basic protein antibody (anti-MBP) variation and myelinoclasis in the brain stem following brain trauma.


METHODS
In rat models of brain trauma, MBP content and anti-MBP titer in the blood were measured using enzyme-linked immunosorbent assay (ELISA) at different time points after brain trauma, and the degree of myelinoclasis in the brain stem slices was assessed with osmic acid staining.


RESULTS
Early after brain trauma, MBP content in the blood increased followed by significant reduction 10 days later. Four days after the trauma, anti-MBP titer was markedly increased, accompanied by obvious exacerbation of myelinoclasis in the brain stem, both reaching the highest levels on day 10, at the point of which anti-MBP titer increased by 4 folds and the number of myelinoclasis by 10 folds compared with the control group. Anti-MBP titer and brain stem myelinolysis both lowered 30 days later. Correlation analysis showed an intimate positive correlation between anti-MBP titer and the degree of myelinoclasis.


CONCLUSIONS
After brain trauma, MBP is released as a specific antigen into the blood to stimulate the immune system for anti-MBP production, and the antibody is intimately related to the brain stem myelinoclasis.
Li
Wei
W
Department of Neurosurgery, First Affiliated Hospital, Jinan University, Guangzhou 510630, China. tliwei@jnu.edu.cn
Chen
Shan-Cheng
SC
Wang
Zhi-Gang
ZG
Song
Xiu-Bao
XB
Wang
Yu-Ping
YP
Zhang
Mei
M
D000818:Animals; D000906:Antibodies / Q000378:metabolism; D001930:Brain Injuries / Q000150:complications*; D001933:Brain Stem / Q000276:immunology* / Q000473:pathology; D020278:Demyelinating Autoimmune Diseases, CNS / Q000209:etiology / Q000276:immunology*; D005260:Female; D008297:Male; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins / Q000097:blood / Q000276:immunology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D014157:Transcription Factors / Q000097:blood / Q000276:immunology*
D000906:Antibodies; C499048:MBP protein, human; D004676:Myelin Basic Protein; D009419:Nerve Tissue Proteins; D014157:Transcription Factors