Peptide substrate for caspase-3 with 2-aminoacridone as reporting group.
Synthesis and properties of a new fluorescent/fluorogenic substrate Ac-DEVD-AMAC for caspase-3 are reported. The substrate is obtained by conventional Fmoc-based solid phase peptide synthesis and its properties are investigated with regard to fluorescence, sensitivity, applicability and kinetic constants. A non-traditional approach to assay the proteases activity using 2-aminoacridone labeled peptides is proposed. This approach utilizes the decrease of fluorescence intensity of a sample as a measure for the enzyme activity.
Lozanov
Valentin
V
Department of Chemistry and Biochemistry, Medical University of Sofia, 2 Zdrave str, 1431 Sofia, Bulgaria. lozanov@medfac.acad.bg
Ivanov
Ivaylo P
IP
Benkova
Bistra
B
Mitev
Vanio
V
D054831:Acridones / Q000138:chemical synthesis / Q000737:chemistry*; D000609:Aminoacridines / Q000138:chemical synthesis / Q000737:chemistry*; D053148:Caspase 3 / Q000032:analysis* / Q000737:chemistry; D002460:Cell Line; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D009842:Oligopeptides / Q000138:chemical synthesis / Q000737:chemistry*; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000379:methods
D054831:Acridones; D000609:Aminoacridines; D005456:Fluorescent Dyes; D009842:Oligopeptides; C540557:acetyl-aspartyl-glutamyl-valyl-aspartyl-2-aminoacridone; C072515:2-aminoacridone; D053148:Caspase 3