Myricetin, a naturally occurring flavonoid, prevents 2-deoxy-D-ribose induced dysfunction and oxidative damage in osteoblastic MC3T3-E1 cells.
Myricetin, a naturally occurring flavonoid, was investigated to determine whether it could protect osteoblasts from 2-deoxy-d-ribose induced dysfunction and oxidative damage in the MC3T3-E1 cells. MC3T3-E1 cells were incubated with 2-deoxy-d-ribose and/or myricetin, and markers of osteoblast function and oxidative damage were examined. Compared with control incubation, 2-deoxy-d-ribose significantly (P<0.05) inhibited alkaline phosphatase (ALP) activity, collagen content, and calcium deposition at the concentration of 20 mM. Cellular malondialdehyde (MDA), protein carbonyl, and advanced oxidation protein products contents were significantly (P<0.05) increased in the presence of 2-deoxy-d-ribose (20 mM). Myricetin significantly (P<0.05) increased cell survival, ALP activity, collagen, osteocalcin, osteoprotegerin, and calcium deposition and decreased MDA, protein carbonyl, and advanced oxidation protein products contents of osteoblastic MC3T3-E1 cells in the presence of 20 mM 2-deoxy-d-ribose. These results demonstrate that myricetin attenuates 2-deoxy-d-ribose induced damage, suggesting that myricetin may be a useful dietary supplement for minimizing oxidative injury in diabetes related bone diseases.
Lee
Kyung-Hee
KH
Department of Food Service Management, College of Hotel and Tourism Management, Kyung Hee University, Seoul 130-701, Republic of Korea.
Choi
Eun-Mi
EM
D000469:Alkaline Phosphatase / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001847:Bone Diseases / Q000188:drug therapy / Q000209:etiology; D002118:Calcium / Q000378:metabolism; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003094:Collagen / Q000187:drug effects / Q000378:metabolism; D003855:Deoxyribose / Q000633:toxicity*; D048909:Diabetes Complications / Q000503:physiopathology / Q000517:prevention & control; D019587:Dietary Supplements; D005419:Flavonoids / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D010006:Osteoblasts / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D050050:Protein Carbonylation / Q000187:drug effects
D005419:Flavonoids; D008315:Malondialdehyde; D003855:Deoxyribose; C040015:myricetin; D003094:Collagen; D000469:Alkaline Phosphatase; D002118:Calcium