Animal shed Bacillus licheniformis spores possess allergy-protective as well as inflammatory properties.
BACKGROUND
Numerous epidemiologic studies have demonstrated an allergy-protective effect of farm life early in childhood. It has been hypothesized that environmental exposure to microbes may contribute to this effect. Because of their small size and thereby their potential for deposition in lower airways of small children, bacterial spores may be candidates for such allergy-protective effects.


OBJECTIVE
To investigate immune responses elicited by exposure to Bacillus spores in experimental settings.


METHODS
Animal shed and mattress dusts were analyzed for bacteria and fungi by aerobic and anaerobic growth. Bacillus licheniformis, the most prominent microorganism found in these samples, was investigated with respect to spore specific stimulation of pattern recognition receptors, monocyte-derived dendritic cells and T(H)-cell polarization in vitro as well as to the prevention of asthma development in a mouse model of allergic asthma.


RESULTS
In vitro, B. licheniformis spores activated a T(H)1 cytokine expression profile. In vivo application of these spores resulted in less spore-specific but long-lasting immune activation preventing eosinophilia and goblet cell hyperplasia; however, they provoked an influx of neutrophils in lung tissue of asthmatic mice.


CONCLUSIONS
Bacterial spores may contribute to the allergy-protective properties of farming environments, but their persistence in the lung causes ongoing immune activation in mouse experiments.
Vogel
Kay
K
Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany.
Blümer
Nicole
N
Korthals
Melanie
M
Mittelstädt
Jessica
J
Garn
Holger
H
Ege
Markus
M
von Mutius
Erika
E
Gatermann
Sören
S
Bufe
Albrecht
A
Goldmann
Torsten
T
Schwaiger
Karin
K
Renz
Harald
H
Brandau
Sven
S
Bauer
Johann
J
Heine
Holger
H
Holst
Otto
O
D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000517:prevention & control; D001407:Bacillus / Q000276:immunology* / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000276:immunology / Q000382:microbiology; D003713:Dendritic Cells / Q000276:immunology / Q000378:metabolism; D004391:Dust / Q000276:immunology*; D005260:Female; D020397:Goblet Cells / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000517:prevention & control; D007249:Inflammation / Q000276:immunology; D007371:Interferon-gamma / Q000097:blood; D016753:Interleukin-10 / Q000097:blood; D015848:Interleukin-5 / Q000097:blood; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000276:immunology / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D013171:Spores, Bacterial / Q000276:immunology* / Q000378:metabolism; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
D004391:Dust; D015848:Interleukin-5; D016753:Interleukin-10; D007371:Interferon-gamma