title: Capillary electrophoretic determination of main components of natural dyes with MS detection.
abstract: CE with UV-Vis and MS detections was investigated as a technique for detection of main components of selected natural dyes of plant and insect origin. The BGE giving the best separation of the investigated flavonoids and anthraquinoids, suitable for MS detection consisted of 40 mM ammonium acetate solution of pH 9.5 with 40% ACN. LODs obtained with MS detection were even one order of magnitude lower than the ones obtained with UV-Vis detection. Application of MS detection enabled determination of eleven dye compounds from three different chemical groups in 15 min. and proved to be more satisfactory than diode-array detection in the electrophoretic analysis of main classes of natural dyes both in terms of selectivity and sensitivity of analysis.
authors.affiliation: Department of Chemistry, Warsaw University, Pasteura, Warsaw, Poland. isurow@chem.uw.edu.pl
authors.forename: Izabella
authors.initials: I
authors.lastname: Surowiec
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pawelec
authors.forename: Melinda
authors.initials: M
authors.lastname: Rezeli
authors.forename: Ferenc
authors.initials: F
authors.lastname: Kilar
authors.forename: Marek
authors.initials: M
authors.lastname: Trojanowicz
mesh_terms: D000818:Animals; D000880:Anthraquinones / Q000032:analysis; D004396:Coloring Agents / Q000032:analysis*; D019075:Electrophoresis, Capillary / Q000379:methods* / Q000706:statistics & numerical data; D005419:Flavonoids / Q000032:analysis; D007202:Indicators and Reagents; D007313:Insecta / Q000737:chemistry; D013058:Mass Spectrometry / Q000379:methods*; D010944:Plants / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D000880:Anthraquinones; D004396:Coloring Agents; D005419:Flavonoids; D007202:Indicators and Reagents