Synergistic effects of stress and omega-3 fatty acid deprivation on emotional response and brain lipid composition in adult rats.
The aim was to determine the consequences of multi-generational n-3 polyunsaturated fatty acids (PUFA) deficiency on emotional response in rats subjected to maternal separation (MS) as chronic early life stress. Pups fed a control or an n-3 PUFA deficient diet were daily separated for 2 weeks before weaning. In adult rats, reward response was assessed by sucrose consumption and reactivity to novelty using openfield test. Both n-3 PUFA deficiency and MS increased reward response and impulsivity. Moreover, nutritional deficiency and stress acted in synergy to elevate sucrose intake by 80%, compared to control conditions. n-3 PUFA deprivation induced a depletion of docosahexanoeic acid of brain membranes by 70% compensated by increase in 22:5 n-6 and arachidonic acid (AA) levels. The diet-induced AA increase was, however, significantly higher in MS rats. This suggests that n-3 PUFA deficit could be an environmental risk increasing vulnerability to depressive-like response induced by chronic stress.
Mathieu
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Unité de Nutrition et Régulation Lipidiques des Fonctions Cérébrales, NuRéLiCe, INRA Domaine de Vilvert, 78350 Jouy en Josas, France. geraldine.mathieu@jouy.inra.fr
Denis
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S
Lavialle
M
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Vancassel
S
S
D000818:Animals; D000831:Animals, Newborn; D016718:Arachidonic Acid / Q000032:analysis; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D001835:Body Weight / Q000502:physiology; D001923:Brain Chemistry / Q000187:drug effects* / Q000502:physiology; D003863:Depression / Q000378:metabolism / Q000503:physiopathology*; D003891:Desipramine / Q000494:pharmacology; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000378:metabolism; D004281:Docosahexaenoic Acids / Q000032:analysis; D004327:Drinking Behavior / Q000187:drug effects / Q000502:physiology; D004435:Eating / Q000502:physiology; D005227:Fatty Acids / Q000032:analysis; D015525:Fatty Acids, Omega-3 / Q000008:administration & dosage* / Q000378:metabolism; D005260:Female; D005526:Food, Formulated; D008055:Lipids / Q000032:analysis* / Q000737:chemistry; D008297:Male; D008426:Maternal Deprivation; D010714:Phosphatidylethanolamines / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D013315:Stress, Psychological / Q000150:complications / Q000378:metabolism* / Q000503:physiopathology*; D013395:Sucrose / Q000008:administration & dosage; D014868:Water Deprivation / Q000502:physiology
D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D008055:Lipids; D010714:Phosphatidylethanolamines; D004281:Docosahexaenoic Acids; D016718:Arachidonic Acid; D013395:Sucrose; D003891:Desipramine