A high throughput protein formulation platform: case study of salmon calcitonin.
OBJECTIVE
The feasibility of using high throughput spectroscopy for characterization and selection of physically stable protein formulations was studied.


METHODS
A hundred aqueous formulations of salmon calcitonin (sCT) were prepared using 20 buffer compositions. The solutions had pH values between 2.5 and 10.5. The stability of the sCT formulations was analyzed over 1 week by the following assays: (1) protein concentration, (2) volume control by measuring pathlength, (3) turbidity (absorbance at 350 nm), (4) intrinsic tyrosine fluorescence, (5) 1-anilino-naphthalene-8-sulfonate (ANS) fluorescence, (6) Nile Red fluorescence. Addition of the dyes (Nile Red and ANS) was used to study protein conformational changes.


RESULTS
After 1 day, 27 out of the 100 formulations of salmon calcitonin were stable. After 7 days, 12 stable sCT formulations remained. The best salmon calcitonin formulation was in 10 mM sodium acetate buffer with pH values between 3.5 and 5.5.


CONCLUSIONS
The findings are in accordance with the sCT formulations that were patented and used commercially. This can be considered as a proof of concept for the high throughput protein formulation platform.
Capelle
Martinus A H
MA
Department of Pharmaceutics and Biopharmaceutics, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Quai E-Ansermet 30, 1211, Geneva 4, Switzerland.
Gurny
Robert
R
Arvinte
Tudor
T
D000817:Anilino Naphthalenesulfonates / Q000737:chemistry; D050071:Bone Density Conservation Agents / Q000008:administration & dosage / Q000737:chemistry*; D002021:Buffers; D002116:Calcitonin / Q000008:administration & dosage / Q000737:chemistry*; D002626:Chemistry, Pharmaceutical; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005456:Fluorescent Dyes; D006863:Hydrogen-Ion Concentration; D010078:Oxazines; D019999:Pharmaceutical Solutions; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet
D000817:Anilino Naphthalenesulfonates; D050071:Bone Density Conservation Agents; D002021:Buffers; D005456:Fluorescent Dyes; D010078:Oxazines; D019999:Pharmaceutical Solutions; C028815:salmon calcitonin; D002116:Calcitonin; C044808:nile red