A rapid and robust method for simultaneously measuring changes in the phytohormones ABA, JA and SA in plants following biotic and abiotic stress.
We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of MS and uses multiple reaction monitoring and isotopically labelled samples to quantify the phytohormones abscisic acid, jasmonic acid and salicylic acid in Arabidopsis leaf tissue.
Forcat
Silvia
S
School of Biosciences, University of Exeter, Stocker Road, Exeter, EX4 4QD, UK. M.R.Grant@exeter.ac.uk.
Bennett
Mark H
MH
Mansfield
John W
JW
Grant
Murray R
MR