Forced-flow bioreactor for sucrose inversion using ceramic membrane activated by silanization.
A forced-flow enzyme membrane reactor system for sucrose inversion was investigated using three ceramic membranes having different pore sizes. Invertase was immobilized chemically to the inner surface of a ceramic membrane activated by a silane-glutaraldehyde technique. With the cross-flow filtration of sucrose solution, the reaction rate was a function of the permeate flux, easily controlled by pressure. Using 0.5 microm support pore size of membrane, the volumetric productivity obtained was 10 times higher than that in a reported immobilized enzyme column reactor, with a short residence time of 5 s and 100% conversion of the sucrose inversion.
Nakajima
M
M
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, 305 Japan.
Watanabe
A
A
Jimbo
N
N
Nishizawa
K
K
Nakao
S
S