title: Integrin-laminin interactions controlling neurite outgrowth from adult DRG neurons in vitro.
abstract: A prerequisite for axon regeneration is the interaction between the growth cone and the extracellular matrix (ECM). Laminins are prominent constituents of ECM throughout the body, known to support axon growth in vitro and in vivo. The regenerative capacity of adult neurons is greatly diminished compared to embryonic or early postnatal neurons. Since most lesions in the nervous system occur in the adult, we have examined neurite outgrowth from adult mouse DRG neurons on four laminin isoforms (laminin-1/LM-111, laminin-2/LM-211, laminin-8/LM-411 and laminin-10/LM-511) in vitro. The growth on laminin-1 and -10 was trophic factor-independent and superior to the one on laminin-2 and -8, where growth was very poor in the absence of neurotrophins. Among other ECM proteins, laminins were by far the most active molecules. Using function-blocking antibodies to laminin-binding integrins, we identified non-overlapping functions of integrins alpha3beta1, alpha7beta1 and alpha6beta1 on different laminin isoforms, in that alpha3beta1 and alpha7beta1 integrins appeared to be specific receptors for both laminin-1 and-2, whereas integrin alpha6beta1 was a receptor for laminin-8 and-10. Lastly, by use of immunohistochemistry, expression of subunits of laminin-1, -2, -8 and -10 in sensory organs in the human epidermis could be demonstrated, supporting an important role for these laminins in relation to primary sensory axons.
authors.affiliation: Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden. stefan.plantman@ki.se
authors.forename: Stefan
authors.initials: S
authors.lastname: Plantman
authors.forename: Manuel
authors.initials: M
authors.lastname: Patarroyo
authors.forename: Kaj
authors.initials: K
authors.lastname: Fried
authors.forename: Anna
authors.initials: A
authors.lastname: Domogatskaya
authors.forename: Karl
authors.initials: K
authors.lastname: Tryggvason
authors.forename: Henrik
authors.initials: H
authors.lastname: Hammarberg
authors.forename: Staffan
authors.initials: S
authors.lastname: Cullheim
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000502:physiology; D005109:Extracellular Matrix / Q000737:chemistry / Q000378:metabolism; D005727:Ganglia, Spinal / Q000166:cytology*; D006801:Humans; D039201:Integrin alpha3beta1 / Q000235:genetics / Q000378:metabolism*; D039121:Integrin alpha6beta1 / Q000235:genetics / Q000378:metabolism*; D016023:Integrins / Q000235:genetics / Q000378:metabolism*; D007797:Laminin / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D009416:Nerve Regeneration / Q000502:physiology; D016501:Neurites / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism*
chemical_list: D039201:Integrin alpha3beta1; D039121:Integrin alpha6beta1; D016023:Integrins; D007797:Laminin; D020033:Protein Isoforms; C082908:integrin alpha7beta1