Identification and characterization of Bacillus anthracis spores by multiparameter flow cytometry.
In response to the need for methods that can rapidly detect potentially virulent Bacillus anthracis spores, we developed a two-color flow cytometric assay capable of simultaneously identifying B. anthracis spores and the presence of spore-associated protective antigen, a virulence marker for strains harboring the pXO1 plasmid.
Schumacher
William C
WC
Department of Chemistry, Ohio State University, Columbus, Ohio 43210, USA.
Storozuk
Craig A
CA
Dutta
Prabir K
PK
Phipps
Andrew J
AJ
D000942:Antigens, Bacterial / Q000032:analysis*; D001408:Bacillus anthracis / Q000302:isolation & purification*; D001427:Bacterial Toxins / Q000032:analysis*; D005434:Flow Cytometry / Q000379:methods*; D046529:Microscopy, Electron, Transmission; D008856:Microscopy, Fluorescence; D010957:Plasmids; D013171:Spores, Bacterial / Q000302:isolation & purification
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; C030325:anthrax toxin