Utility of antiPax5 in the diagnosis of lymphoproliferative disorders and neoplasia in mice.
CD45R/B220 antigen (B220) is a common mouse panB-cell marker used for paraffin-embedded tissues. However, antiB220 has limited specificity in diagnostic pathology because the B220 antigen is expressed on subsets of cytotoxic T lymphocytes and natural killer cells, on plasmacytic dendritic cells, and on T lymphocytes of mice with the lymphoproliferative disorder associated with Fas (lymphoproliferative mutant mouse, B6.MRL-Fas(lpr/J)) or Fas ligand (generalized lymphoproliferative disease mutant mouse, C3H/ HeJ-Fasl(gld/J) or B6Smn.C3-Fasl(gld/J)). In addition, mouse B lymphocytes vary in the amount of B220 expressed, and some subsets of mouse B lymphocytes do not express B220 at all. In comparison, Pax5 expression (detected by immunohistochemistry using antiPax5) offers greater specificity and sensitivity because of its earlier expression during B-cell differentiation, its ability to detect all committed B cells, and its restriction to the B-cell lineage. Here we describe the use of an antibody to human Pax5 in diagnostic pathology with formalin-fixed, paraffin-embedded mouse tissue.
Rehg
Jerold E
JE
St Jude Children's Research Hospital, Pathology Department, Memphis, TN, USA. jerold.rehg@stjude.org
Sundberg
John P
JP
D000818:Animals; D007150:Immunohistochemistry; D008232:Lymphoproliferative Disorders / Q000175:diagnosis*; D051379:Mice; D008809:Mice, Inbred C3H; D009374:Neoplasms, Experimental / Q000175:diagnosis* / Q000473:pathology; D051757:PAX5 Transcription Factor / Q000037:antagonists & inhibitors*
D051757:PAX5 Transcription Factor; C497282:Pax5 protein, mouse