title: Evaluation of techniques for detection of small trematode eggs in faeces of domestic animals.
abstract: This study was conducted to evaluate techniques for detection of small trematode eggs in faeces of dogs, cats and pigs. Faecal samples from dogs (n=80), cats (n=35) and pigs (n=114) were examined by Kato-Katz technique (KK), formalin-ether sedimentation technique (FE) and a method of combining: filtration, sedimentation and centrifugation, developed at DBL - Centre for Health Research and Development (former Danish Bilharziasis Laboratory) (DBL). Necropsy was performed on 38 dogs, 25 cats and 16 pigs and was considered as a gold standard method for evaluation of the techniques. The results showed 100% specificity for the three techniques. Lower sensitivity was seen for the KK-technique in dog samples in comparison to that for DBL- and FE-technique. The sensitivity of the three techniques was similar in cats and pigs. Based on these findings and practical issues, DBL-technique was chosen as most suitable because the eggs were easily detected and quantified. No toxic chemicals or special equipment were required in comparison with FE-technique that needs ether solution and thus fume cupboards which are often unavailable in local veterinary centres in Vietnam and other developing countries.
authors.affiliation: National Institute of Veterinary Research, Hanoi, Viet Nam. linhlananh2001@yahoo.com
authors.forename: Nguyen Thi Lan
authors.initials: NT
authors.lastname: Anh
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Phuong
authors.forename: Giang Hoang
authors.initials: GH
authors.lastname: Ha
authors.forename: Luong To
authors.initials: LT
authors.lastname: Thu
authors.forename: Maria Vang
authors.initials: MV
authors.lastname: Johansen
authors.forename: Darwin K
authors.initials: DK
authors.lastname: Murrell
authors.forename: Stig Milan
authors.initials: SM
authors.lastname: Thamsborg
mesh_terms: D000818:Animals; D002371:Cat Diseases / Q000175:diagnosis* / Q000469:parasitology; D002415:Cats; D004283:Dog Diseases / Q000175:diagnosis* / Q000469:parasitology; D004285:Dogs; D010063:Ovum; D010270:Parasite Egg Count / Q000379:methods*; D012680:Sensitivity and Specificity; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis* / Q000469:parasitology; D014200:Trematoda / Q000502:physiology*