Differential feeding responses to central alpha-melanocyte stimulating hormone in genetically low and high body weight selected lines of chickens.
This study was conducted to compare the effects of central alpha-MSH, a potent anorexigenic signal, in lines of chickens that have undergone long-term divergent selection for low (LWS) or high (HWS) body weight. Chicks from both lines were centrally injected with 0, 24, 120 or 600 pmol alpha-MSH and feed and water intake were concurrently measured thereafter for a total of 180 min. The LWS line responded to all doses of alpha-MSH with a similar potent decrease in feed intake at all observation times. The HWS line only responded to 600 pmol alpha-MSH with decreased feed intake. alpha-MSH did not influence water intake in either line. To determine if differential hypothalamic signaling was associated with the anorexigenic effect, c-Fos immunoreactivity was measured in appetite-related hypothalamic nuclei after 600 pmol central alpha-MSH injections. c-Fos immunoreactivity was increased in the dorsomedial hypothalamus, paraventricular nucleus (PVN) and ventromedial hypothalamus in both lines after alpha-MSH; however, the magnitude of increase was greater in LWS than in HWS chicks at the PVN (136% vs. 47% increase over controls, respectively). Based on behavior observations, the number of feeding and exploratory pecks is decreased with greater magnitude after alpha-MSH in the LWS line. Additionally, alpha-MSH was associated with increased deep rest in both lines, and may be a secondary effect to reduced ingestion. These data support that the LWS line has a lower threshold for the anorexigenic effect of central alpha-MSH while in the HWS line this threshold is higher, and that this difference may be associated with differential hypothalamic signaling. Genetic variation exists in the threshold of anorexigenic response for central alpha-MSH in LWS and HWS lines of chickens with possible implications to other species including humans.
Cline
Mark A
MA
Department of Biology, Radford University, Radford, VA 24142, USA. mcline@RADFORD.EDU
Nandar
Wint
W
Bowden
Christie
C
Hein
Pyae Phyo
PP
Denbow
D Michael
DM
Siegel
Paul B
PB
D000818:Animals; D001835:Body Weight / Q000235:genetics*; D001921:Brain / Q000187:drug effects*; D002645:Chickens; D004326:Drinking / Q000187:drug effects; D004435:Eating / Q000187:drug effects*; D007150:Immunohistochemistry; D007276:Injections, Intraventricular; D016760:Proto-Oncogene Proteins c-fos / Q000032:analysis; D000521:alpha-MSH / Q000494:pharmacology*
D016760:Proto-Oncogene Proteins c-fos; D000521:alpha-MSH