title: Fed-batch fermentation for the production of alpha-amylase by Bacillus amyloliquefaciens.
abstract: Fed-batch cultures were performed to maximize the alpha-amylase activity in a bioreactor. Kinetic equations containing a catabolite repression effect were used to model the enzyme formation from Bacillus amyloliquefaciens. Fed-batch culture experiments were performed using maltose to implement the optimal feeding strategy. Optimal fed-batch culture based on sequential parameter estimation was performed successfully using off-line analysis while the fermentation was in progress. The enzyme activity from the fed-batch culture employing maltose was higher than that of the batch culture by 60%. Enzyme production using starch showed similar trends to those obtained using maltose.
authors.affiliation: Department of Chemical and Nuclear Engineering, University of Maryland, College Park, Maryland 20742.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Yoo
authors.forename: T W
authors.initials: TW
authors.lastname: Cadman
authors.forename: J
authors.initials: J
authors.lastname: Hong
authors.forename: R T
authors.initials: RT
authors.lastname: Hatch