Oligomerization of Vibrio cholerae hemolysin induces CXCR3 upregulation and activation of B-1a cell.
The hemolysin oligomer promotes the proliferation of B-1a cells and the expression of CD25, which is indicative of cell activation, on B-1a cells. The upregulation of CD86 induced by the oligomer showed its selective bias for the B7-2 member of B7 family while the monomer failed to induce these effects. The oligomer induced the expression of CXCR3, associated with B cell activation, while the monomer induced the expression of CXCL4, a powerful angiostatic chemokine. In conclusion, we found that B-1a cells responded to the apoptogenic monomer by expressing CXCL4, whereas oligomerization of the immunogen induced CXCR3 to shift the response towards activation.
Mukherjee
Gayatri
G
Divisions of Immunology and Biochemistry, National Institute of Cholera and Enteric Diseases, P-33, Scheme XM, Kolkata, West Bengal, India.
Banerjee
Kalyan K
KK
Biswas
Tapas
T
D000818:Animals; D001402:B-Lymphocytes / Q000276:immunology*; D051940:B7-2 Antigen / Q000276:immunology / Q000378:metabolism; D001426:Bacterial Proteins / Q000276:immunology / Q000378:metabolism*; D002478:Cells, Cultured; D006460:Hemolysin Proteins / Q000276:immunology / Q000378:metabolism*; D008213:Lymphocyte Activation; D051379:Mice; D010978:Platelet Factor 4 / Q000276:immunology / Q000378:metabolism; D054367:Receptors, CXCR3 / Q000276:immunology / Q000378:metabolism*; D015854:Up-Regulation; D014734:Vibrio cholerae / Q000276:immunology* / Q000378:metabolism
D051940:B7-2 Antigen; D001426:Bacterial Proteins; C515188:Cxcr3 protein, mouse; D006460:Hemolysin Proteins; D054367:Receptors, CXCR3; C073845:hemolysin, Vibrio; D010978:Platelet Factor 4