FRET-based aptamer probe for rapid angiogenin detection.
A sensitive method for rapid angiogenin (Ang) detection based on fluorescence resonance energy transfer (FRET) has been described. A dual-labeled probe based on high affinity aptamer for Ang was constructed. As donor and acceptor, 6-carboxyfluorescein (FAM) and 6-carboxy-tetramethylrhodamine (TMR) were labeled at 5'- and 3'-termini of the aptamer probe, respectively. The dual-labeled probe showed obvious fluorescence changes due to the specific binding between aptamer and Ang. By monitoring the fluorescence intensity of donor and acceptor, quantitative Ang detection could be achieved. This assay is highly specific and sensitive, with a detection limit of 2.0 x 10(-10) mol L(-1) and a linear range of 5.0 x 10(-10) to 4.0 x 10(-8) mol L(-1) Ang. Ang in serum samples of health and lung cancer were also detected.
Li
Wei
W
Engineering Center for Biomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Yang
Xiaohai
X
Wang
Kemin
K
Tan
Weihong
W
Li
Huimin
H
Ma
Changbei
C
D043925:Angiogenesis Inducing Agents / Q000032:analysis / Q000097:blood; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood*; D015336:Molecular Probe Techniques / Q000295:instrumentation*; D008969:Molecular Sequence Data; D009363:Neoplasm Proteins / Q000032:analysis / Q000097:blood*; D015203:Reproducibility of Results; D012259:Ribonuclease, Pancreatic / Q000032:analysis / Q000097:blood*; D012680:Sensitivity and Specificity; D013997:Time Factors
D043925:Angiogenesis Inducing Agents; D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D009363:Neoplasm Proteins; C046366:angiogenin; D012259:Ribonuclease, Pancreatic