title: Qualitative and quantitative determination of the major coumarins in Zushima by high performance liquid chromatography with diode array detector and mass spectrometry.
abstract: A method, high-performance liquid chromatography coupled with diode array and electrospray tandem mass spectrometry (HPLC-DAD-ESI-MS), was developed to qualitatively identify and quantitatively determine the 10 major active coumarins of Zushima. The analysis was performed by using a ZORBAX SB-C18 analytical column (250 mm x 4.6 mm ID, 5 microm) at gradient elution of 0.5% aqueous formic acid and acetonitrile with diode array detection (325 nm). The method was validated for linearity, precision, accuracy, limit of detection and quantification. The proposed method was successfully applied for the qualitative and quantitative analysis of 10 coumarins in five different species of Zushima which had great variation on the contents of investigated coumarins.
authors.affiliation: Department of Natural Medicinal Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Su
authors.forename: Chuan
authors.initials: C
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Yun-heng
authors.initials: YH
authors.lastname: Shen
authors.forename: Hui-liang
authors.initials: HL
authors.lastname: Li
authors.forename: Run-hui
authors.initials: RH
authors.lastname: Liu
authors.forename: Xi
authors.initials: X
authors.lastname: Zhang
authors.forename: Xiao-jia
authors.initials: XJ
authors.lastname: Hu
authors.forename: Wei-dong
authors.initials: WD
authors.lastname: Zhang
mesh_terms: D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis*; D032364:Daphne / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal