Sex hormones affect the production of recombinant Factor IX in CHO and HEK-293 cell lines.
Recombinant human Factor IX (rFIX) was cloned in a mammalian expression vector and transfected into CHO and HEK-293. Treatment with 10(-9) M methyl testosterone increased rFIX production by 30-50% in CHO and HEK clones. However, 10(-9) M 17beta-oestradiol increased production of rFIX by ~50% in CHO-F7 clone and decreased production by 48% and 37% in CHO-F8 and HEK-F2-6, respectively. Progesterone treatment inhibited rFIX production in both cell lines. Production of rFIX can thus be increased by sex hormone treatment and therefore used to enhance biotechnological production in mammalian cells.
Dadehbeigi
Nazanin
N
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Ostad
Seyed Nasser
SN
Faramarzi
Mohammad Ali
MA
Ghahremani
Mohammad Hossein
MH
D000818:Animals; D016466:CHO Cells; D002460:Cell Line; D006224:Cricetinae; D003412:Cricetulus; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D004958:Estradiol / Q000494:pharmacology; D005164:Factor IX / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression / Q000187:drug effects; D012739:Gonadal Steroid Hormones / Q000494:pharmacology*; D006801:Humans; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D013739:Testosterone / Q000031:analogs & derivatives / Q000494:pharmacology; D014162:Transfection
D012739:Gonadal Steroid Hormones; D011994:Recombinant Proteins; D013739:Testosterone; D004958:Estradiol; D005164:Factor IX