Kinetic expression for maltose production from soluble starch by simultaneous use of beta-amylase and debranching enzymes.
The kinetics of the hydrolysis of soluble starch by simultaneous use of beta-amylase and either isoamylase or pullulanse was studied experimentally for a wide range of subtrate and enzyme concentrations. A kinetic expression was constituted for maltose production by beta-armylase, which was stimulated by an increase in linear linkage portions due to the debranching enzyme on amylopectin molecules. As a result, calculations by the kinetic expression agreed with time course data under various conditions.
Shiraishi
F
F
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Higashi-ku, Fukuoka 812, Japan.
Kawakami
K
K
Yuasa
A
A
Kojima
T
T
Kusunoki
K
K