title: GDNF isoform affects intracellular trafficking and secretion of GDNF in neuronal cells.
abstract: Glial cell line derived neurotrophic factor (GDNF) plays a critical role in central and peripheral neuron survival and function. In human and rodents, GDNF exists in an alternative spliced isoform (GDNF Delta 78), which has a 78 bp deletion in the pro-region of the GDNF encoding sequence. Whether the GDNF isoform affects GDNF function is unknown. Here, we investigated the secretion and intracellular localization of the GDNF Delta 78 isoform in neuronal cell populations. Our data indicate that a decreased secretion and an abnormal intracellular distribution of GDNF Delta 78 occurred in neuronal cells. The colocalization studies revealed much more localization of GDNF Delta 78 with Golgi marker-TGN38, which indicates that the accumulation of GDNF Delta 78 in the Golgi apparatus might in part account for its intracellular trafficking and secretion deficit. To our knowledge, it is reported for the first time that the GDNF Delta 78 isoform has a deficit in GDNF intracellular trafficking and secretion.
authors.affiliation: Department of Neurobiology, School of Medicine, Shandong University, No.44 Wenhua Xi Road, Jinan, Shandong 250012, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Wang
authors.forename: Zhao
authors.initials: Z
authors.lastname: Geng
authors.forename: Ling
authors.initials: L
authors.lastname: Zhao
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Huang
authors.forename: Ai-Li
authors.initials: AL
authors.lastname: Sheng
authors.forename: Zhe-Yu
authors.initials: ZY
authors.lastname: Chen
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology; D004622:Embryo, Mammalian; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005260:Female; D051100:Glial Cell Line-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D006056:Golgi Apparatus / Q000187:drug effects / Q000378:metabolism; D006624:Hippocampus / Q000166:cytology; D006801:Humans; D047468:Immunoprecipitation / Q000379:methods; D008562:Membrane Glycoproteins / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000502:physiology / Q000648:ultrastructure; D011247:Pregnancy; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D021381:Protein Transport / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D014162:Transfection
chemical_list: D051100:Glial Cell Line-Derived Neurotrophic Factor; D008562:Membrane Glycoproteins; D020033:Protein Isoforms; C513045:Tgoln2 protein, rat