An adaptive state estimator for detecting contaminants in bioreactors.
An algorithm is presented for detecting the appearance of contaminants during batch or fed-batch fermentations, using only presently available on-line measurements. Its adaptive nature enables it to rely on almost no prior knowledge of the real process. The necessary on-line measurements are total biomass and its production rate; it is also shown how a physical variable such as oxygen uptake can be used alone instead. The algorithm's properties are studied theoretically and through simulations. These were confirmed by on-line experimental results, obtained with a Yeast culture, both pure and contaminated by a Bacteria. The algorithm does not detect contaminants when none are there, and it also provides a convergent estimate of a pure culture's specific growth rate. Contaminated cultures are recognized by the algorithm, and this detection can be made more or less conservative. After detection, the various estimates may diverge, due to general observability difficulties, though this divergence can itself be monitored. Moreover, the algorithm is easy to tune and its qualitative behavior is quite insensitive to its adjustable parameters. A practical criterion and scheme for implementation are proposed. The generality of the approach, which far exceeds the experimental system used, is finally discussed.
Chattaway
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Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Stephanopoulos
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