Laccase mediated biodegradation of 2,4-dichlorophenol using response surface methodology.
The effects of different environmental parameters, i.e., pH, temperature, time and enzyme concentration on the biodegradation of 2,4-dichlorophenol (2,4-DCP) in aqueous phase was evaluated with laccase from Pleurotus sp. using response surface methodology (RSM) in the present investigation. The Box-Behnken design of experiments was used to construct second order response surfaces with the investigated parameters. It was observed that the maximum degradation efficiency of approximately 98% was achieved at pH 6, temperature of 40 degrees C, time 9h and an enzyme concentration of 8 IU ml(-1). The adequacy of the model was confirmed by the coefficient of multiple regression, R(2) and adjusted R(2) which were adjudged to be 87.9% and 73.6%, respectively indicating a reasonably good model for practical implementation. Despite the fact that many successful attempts have been taken in the past for biodegradation of 2,4-DCP using whole cells, the present study emphasizes the fastest biodegradation of 2,4-DCP, a potent xenobiotic compound.
Bhattacharya
S S
SS
Microbial Biotechnology and Downstream Processing Laboratory, Indian Institute of Technology, Kharagpur 721302, India.
Banerjee
R
R
D001673:Biodegradation, Environmental; D002733:Chlorophenols / Q000032:analysis / Q000378:metabolism*; D004785:Environmental Pollutants / Q000032:analysis / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D042845:Laccase / Q000378:metabolism*; D015233:Models, Statistical; D020076:Pleurotus / Q000378:metabolism; D013696:Temperature; D013997:Time Factors; D015262:Xenobiotics / Q000032:analysis
D002733:Chlorophenols; D004785:Environmental Pollutants; D015262:Xenobiotics; D042845:Laccase; C004762:2,4-dichlorophenol