Neuropeptide Y (NPY) Y2 receptors of rabbit kidney cortex are largely dimeric.
The neuropeptide Y (NPY) Y2 receptors and the pancreatic polypeptide Y4 receptors from rabbit kidney cortex are isolated largely as approximately 180 kDa complexes constituted of one receptor dimer and one G-protein heterotrimer, similar to NPY receptors expressed in the Chinese hamster ovary (CHO) cells. As expected, kidney and CHO cell Y2 dimers are converted into monomers by increasing concentrations of a selective agonist. Prevalence of dimeric Y2 receptors in the kidney could be related to low plasma levels of Y2 agonists, and possibly also to a relatively low concentration of Gi alpha subunits.
Estes
A M
AM
Department of Pharmacology, University of Tennessee Health Science Center, Memphis, TN 38163, USA.
Wong
Y Y
YY
Parker
M S
MS
Sallee
F R
FR
Balasubramaniam
A
A
Parker
S L
SL
D000818:Animals; D016466:CHO Cells; D006224:Cricetinae; D003412:Cricetulus; D019281:Dimerization; D019206:GTP-Binding Protein alpha Subunits, Gi-Go / Q000235:genetics / Q000378:metabolism*; D019204:GTP-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D007672:Kidney Cortex / Q000378:metabolism*; D008297:Male; D011485:Protein Binding; D011817:Rabbits; D017476:Receptors, Neuropeptide Y / Q000819:agonists / Q000378:metabolism*; D012995:Solubility
D017476:Receptors, Neuropeptide Y; C095889:neuropeptide Y2 receptor; D019204:GTP-Binding Proteins; D019206:GTP-Binding Protein alpha Subunits, Gi-Go