[Protective effect of Yiqi Mingmu solution on rat's retina with ischemia reperfusion injury].
OBJECTIVE
To investigate the protective effect of the Yiqi Mingmu solution irrigated into rat on retinal injury of rat induced by ischemia reperfusion.


METHODS
SD rats were divided into four groups randomly: negative control group, model control group, low and high dose group. Increasing the intraocular pressure with anterior chambers infused liquid induced the models of retinal ischemia reperfusion of rats. Different doses of Yiqi Mingmu solution were respectively irrigated into rats 7 days before ischemia in low and high dose group. Normal salire instead was used in normal and model control group. At 1, 3, 7, 15 days after the ischemia reperfusion injury, the content of MDA and activity of SOD in rat's retina were measured. The histopathologic changes were observed with transmission electron microscopy and light microscopy.


RESULTS
At 3, 7, 15 days after the ischemia reperfusion injury, the activity of SOD in Yiqi Mingmu solution low and high dose group were higher than in the model control group, the content of MDA in Yiqi Mingmu solution high dose group were lower than in the model control group. The histopathologic injury of retina in the Yiqi Mingmu solution low and high dose group were less than in the model control group.


CONCLUSIONS
Yiqi Mingmu solution has protective effect on rat's retina ischemia reperfusion injury, and it is an effective traditional Chinese recipe to treat the diseases of retina ischemia.
Tang
Xi-lan
XL
Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China. txilan@163.com
Wang
Yan-dong
YD
Huang
Chu-long
CL
Wu
Wei
W
D000818:Animals; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D005260:Female; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008854:Microscopy, Electron; D010946:Plants, Medicinal / Q000737:chemistry*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D012160:Retina / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D012165:Retinal Ganglion Cells / Q000187:drug effects / Q000473:pathology / Q000648:ultrastructure; D013482:Superoxide Dismutase / Q000378:metabolism
D004365:Drugs, Chinese Herbal; C530867:yiqi mingmu; D008315:Malondialdehyde; D013482:Superoxide Dismutase