title: Protocol for the recovery of in vivo matured canine oocytes based on once daily measurement of serum progesterone.
abstract: The collection of in vivo matured canine oocytes relies on the accurate prediction of ovulation. The present study was designed to develop a protocol for the recovery of in vivo matured canine oocytes based on once daily measurements of serum progesterone (P(4)) concentrations. Blood samples (2 mL) were collected every day at 0900 h, and P(4) concentrations were analyzed using a DSL-3900 ACTIVE Progesterone Coated-Tube Radioimmunoassay Kit. The average number of oocytes at the metaphase II (M II) stage was significantly higher at or after 72 h (6.7 to 7.5) compared to 56 h (1.7) following ovulation. The highest numbers of corpora lutea, and therefore the highest numbers of oocytes, were recovered from bitches with initial ovulatory P(4) concentrations ranging from 6.0 to 8.0 ng/ mL (12.2 and 11.4, respectively) compared to from 4.0 to 4.9 ng/ mL (9.6 and 8.8, respectively; p < 0.05). The average number of M II oocytes recovered at 84 h from bitches with initial ovulatory P(4) levels of 5.0 to 5.9 ng/mL (7.7) was higher compared to bitches with P(4) levels of 4.0 to 4.9 ng/ mL (3.5) and 6.0 to 8.0 ng/ mL (4.8; p < 0.05). When oocyte recovery time was adjusted for initial ovulatory P(4) concentration, no significant difference in recovery rates or oocyte quality were observed. In conclusion, once daily measurements of P(4) can be used to predict ovulation in bitches, and oocyte recovery time should be adjusted for initial ovulatory serum P(4) concentrations.
authors.affiliation: Sooam Biotech Research Foundation, Seoul, Republic of Korea.
authors.forename: Mohammad Shamim
authors.initials: MS
authors.lastname: Hossein
authors.forename: Yeon Woo
authors.initials: YW
authors.lastname: Jeong
authors.forename: Sue
authors.initials: S
authors.lastname: Kim
authors.forename: Jeong Joo
authors.initials: JJ
authors.lastname: Kim
authors.forename: Sun Woo
authors.initials: SW
authors.lastname: Park
authors.forename: Chang Soo
authors.initials: CS
authors.lastname: Jeong
authors.forename: Sang Hwan
authors.initials: SH
authors.lastname: Hyun
authors.forename: Woo Suk
authors.initials: WS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D004285:Dogs; D005260:Female; D009865:Oocytes / Q000166:cytology* / Q000502:physiology; D010060:Ovulation; D011374:Progesterone / Q000097:blood*; D011933:Reagent Kits, Diagnostic
chemical_list: D011933:Reagent Kits, Diagnostic; D011374:Progesterone