Online RPLC-GC via TOTAD method to isolate (+)-methyl epijasmonate from lemon (Citrus limon Burm.).
Pure (+)-methyl epijasmonate was isolated from lemon ( Citrus limon Burm.) for the first time. To that aim, two commercial essential oils and one homemade extract were included in the present paper. First, a study on the appropriate chromatographic conditions to avoid the epimerization from methyl epijasmonate to the more stable methyl jasmonate was accomplished. The results obtained are discussed. The presence of (+)-methyl epijasmonate in the three samples studied was initially established through the direct injection into GC-MS. However, the overlapping of (+)-methyl epijasmonate with other matrix components made it necessary to employ a multidimensional technique. RPLC-GC analysis via through-oven transfer adsorption-desorption (TOTAD) provided the selectivity and sensitivity required, reflecting that the homemade lemon extract was an adequate natural source to obtain pure (+)-methyl epijasmonate by means of the collection of the corresponding RPLC fraction.
del Mar Caja
Maria
M
Instituto de Fermentaciones Industriales CSIC, Juan de la Cierva 3, 28006 Madrid, Spain.
Blanch
Gracia Patricia
GP
Ruiz del Castillo
Maria Luisa
ML
D000085:Acetates / Q000302:isolation & purification*; D000327:Adsorption; D002849:Chromatography, Gas / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002957:Citrus / Q000737:chemistry*; D003517:Cyclopentanes / Q000302:isolation & purification*; D005638:Fruit / Q000737:chemistry*; D008401:Gas Chromatography-Mass Spectrometry; D009822:Oils, Volatile / Q000737:chemistry; D054883:Oxylipins / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D013237:Stereoisomerism
D000085:Acetates; D003517:Cyclopentanes; D009822:Oils, Volatile; D054883:Oxylipins; D010936:Plant Extracts; C072239:methyl jasmonate