In vivo burn imaging using Mueller optical coherence tomography.
We report on the use of a high-speed, fiber-based Mueller-matrix optical coherence tomography system with continuous source-polarization modulation for in vivo burn depth evaluation and healing monitoring. A homemade hand-held probe with integrated optical scanning and beam delivering optics was coupled in the sample arm. In vivo burn imaging was demonstrated on porcine skin at different stages of the wound healing process, where porcine skin was used because of its similarity to the human skin. Thermally damaged region was clearly localized in the depth-resolved phase retardation image extracted from the measured Jones matrix. The burn areas in the OCT images agreed well with the histology. By using a decomposition algorithm developed by our group, we also mapped the local birefringence of the skin. The experimental results demonstrate the system's potential for in vivo burn-depth determination.
Todorović
Milos
M
Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843-3120, USA.
Jiao
Shuliang
S
Ai
Jun
J
Pereda-Cubián
David
D
Stoica
George
G
Wang
Lihong V
LV
D000465:Algorithms; D000818:Animals; D001718:Birefringence; D002056:Burns / Q000473:pathology*; D003952:Diagnostic Imaging / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted; D055095:Optics and Photonics; D012038:Regeneration; D012867:Skin / Q000473:pathology; D013552:Swine; D013997:Time Factors; D041623:Tomography, Optical Coherence / Q000295:instrumentation* / Q000379:methods*