Effects of short-term acid and aluminum exposure on the parr-smolt transformation in Atlantic salmon (Salmo salar): disruption of seawater tolerance and endocrine status.
Episodic acidification resulting in increased acidity and inorganic aluminum (Al(i)) is known to interfere with the parr-smolt transformation of Atlantic salmon (Salmo salar), and has been implicated as a possible cause of population decline. To determine the extent and mechanism(s) by which short-term acid/Al exposure compromises smolt development, Atlantic salmon smolts were exposed to either control (pH 6.7-6.9) or acid/Al (pH 5.4-6.3, 28-64 microgl(-1) Al(i)) conditions for 2 and 5 days, and impacts on freshwater (FW) ion regulation, seawater (SW) tolerance, plasma hormone levels and stress response were examined. Gill Al concentrations were elevated in all smolts exposed to acid/Al relative to controls confirming exposure to increased Al(i). There was no effect of acid/Al on plasma ion concentrations in FW however, smolts exposed to acid/Al followed by a 24h SW challenge exhibited greater plasma Cl(-) levels than controls, indicating reduced SW tolerance. Loss of SW tolerance was accompanied by reductions in gill Na(+),K(+)-ATPase (NKA) activity and Na(+),K(+),2Cl(-) (NKCC) cotransporter protein abundance. Acid/Al exposure resulted in decreased plasma insulin-like growth factor (IGF-I) and 3,3',5'-triiodo-l-thyronine (T(3)) levels, whereas no effect of treatment was seen on plasma cortisol, growth hormone (GH), or thyroxine (T(4)) levels. Acid/Al exposure resulted in increased hematocrit and plasma glucose levels in FW, but both returned to control levels after 24h in SW. The results indicate that smolt development and SW tolerance are compromised by short-term exposure to acid/Al in the absence of detectable impacts on FW ion regulation. Loss of SW tolerance during short-term acid/Al exposure likely results from reductions in gill NKA and NKCC, possibly mediated by decreases in plasma IGF-I and T(3).
Monette
Michelle Y
MY
Graduate Program in Organismic and Evolutionary Biology, University of Massachusetts, 611 N. Pleasant Street, Amherst, MA 01003, USA. michelle.monette@yale.edu
Björnsson
Björn Thrandur
BT
McCormick
Stephen D
SD
D000064:Acclimatization / Q000187:drug effects* / Q000502:physiology; D000143:Acids / Q000494:pharmacology*; D000535:Aluminum / Q000494:pharmacology*; D000818:Animals; D001786:Blood Glucose / Q000032:analysis / Q000187:drug effects; D002712:Chlorides / Q000097:blood; D004703:Endocrine System / Q000502:physiology*; D005880:Gills / Q000201:enzymology / Q000378:metabolism; D013006:Growth Hormone / Q000097:blood; D006854:Hydrocortisone / Q000097:blood; D007334:Insulin-Like Growth Factor I / Q000032:analysis; D018978:Molting / Q000187:drug effects*; D020317:Salmo salar / Q000097:blood / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D012623:Seawater; D012964:Sodium / Q000097:blood; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013997:Time Factors
D000143:Acids; D001786:Blood Glucose; D002712:Chlorides; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone; D012964:Sodium; D000535:Aluminum; D000254:Sodium-Potassium-Exchanging ATPase; D006854:Hydrocortisone