Infections with beta-haemolytic streptococci: detection by a universal PCR for bacterial DNA and DNA sequencing.
PCR using universal bacterial primers and subsequent DNA sequence analysis of the PCR product was used to detect beta-haemolytic streptococci in clinical samples. Streptococcus pyogenes, Streptococcus agalactiae and Streptococcus dysgalactiae were identified in 19 primary culture-negative cases of infections from 203 patient samples with detectable bacterial DNA.
Grarup
Cecilie
C
Department of Bacteriology, Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark. ccg@ssi.dk
Gleesen
Ann Sofie
AS
Dargis
Rimtas
R
Andresen
Keld
K
Christensen
Jens Jørgen
JJ
Kemp
Michael
M
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D013290:Streptococcal Infections / Q000175:diagnosis* / Q000382:microbiology; D013291:Streptococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*
D017931:DNA Primers; D004269:DNA, Bacterial