title: Synthesis and secretion of alpha-amylase by rice callus: evidence for differential gene expression.
abstract: Rice seed callus expressed and secreted alpha-amylase at high levels. Twenty percent of the protein secreted by the callus was alphaamylase. The callus secreted about 840 mug alpha-amylase with 10.9 x 10(3) units of activity per gram dry weight callus per day. The alpha-amylase from callus exhibited a more complex isoform pattern than the germinating seed alpha-amylase. In addition, the level of mRNA expression by the five alpha-amylase gene groups was markedly different between callus and the germinating seed. The rice callus culture has features which it attractive as a potential system for expression proteins in plant cell fermentation systems.
authors.affiliation: Department of Genetics, University of California, Davis, California 95616.
authors.forename: C R
authors.initials: CR
authors.lastname: Simmons
authors.forename: N
authors.initials: N
authors.lastname: Huang
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.forename: R L
authors.initials: RL
authors.lastname: Rodriguez