title: Solvent effects on biocatalysis in organic systems: equilibrium position and rates of lipase catalyzed esterification.
abstract: Porcine pancreatic lipase immobilized on celite particles has been employed as a catalyst for the esterification of dodecanol and decanoic acid in a predominantly organic system. Solvent influence on the equilibrium position and on the catalyst activity has been studied using 20 solvents, including aliphatic and aromatic hydrocarbons, ethers, ketones, nitro- and halogenated hydrocarbons, and esters. The equilibrium constant for esterification correlates well with the solubility of water in the organic solvent, which in turn shows a good relationship with a function of Guttman's donor number and the electron pair acceptance index number of the solvent. This may be rationalized in terms of the requirements for solvation of water and of the reactants. The catalyst activity, measured as the initial rate of the esterification reaction, is best correlated as a function of both n-octanol-water partition coefficient (log P) and either the electron pair acceptance index or the polarizability.
authors.affiliation: Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK.
authors.forename: R H
authors.initials: RH
authors.lastname: Valivety
authors.forename: G A
authors.initials: GA
authors.lastname: Johnston
authors.forename: C J
authors.initials: CJ
authors.lastname: Suckling
authors.forename: P J
authors.initials: PJ
authors.lastname: Halling