title: Serum-free media in hybridoma culture and monoclonal antibody production.
abstract: The replacement of serum in hybridoma cultures is considered. The focus is on the effects of serum-free media on hybridoma growth and monoclonal antibody secretion. Comparative literature data with serum supplemented cultures are discussed with an analysis of serum-free formulations and selection rules for the serum-free ingredients. In general, serum-free media which are "lipid rich" can sustain cell growth rates approaching that of serum supplemented cultures. Specific antibody secretion rate, however, is usually higher in serum-free media, irrespective of the lipid content.
authors.affiliation: Biotherapeutics, La Jolla, California 92037, USA.
authors.forename: M C
authors.initials: MC
authors.lastname: Glassy
authors.forename: J P
authors.initials: JP
authors.lastname: Tharakan
authors.forename: P C
authors.initials: PC
authors.lastname: Chau