Simultaneous determination of artemether and lumefantrine in fixed dose combination tablets by HPLC with UV detection.
This paper describes the development and validation of a HPLC-UV method (210 nm) for the simultaneous quantitation of artemether and lumefantrine in fixed dose combination tablets. The method showed to be linear (r(2)>0.99), precise (R.S.D.<2.0%), accurate (recovery of 101.07% for artemether and 101.58% for lumefantrine), specific and robust. Four batches of artemether-lumefantrine tablets were assayed by the validated method. The artemether contents in the tablets varied from 98.61% to 103.35%, while lumefantrine contents were 97.92-100.48%.
César
Isabela da Costa
Ida C
Departamento de Produtos Farmacêuticos, Faculdade de Farmácia, Universidade Federal de Minas Gerais, Av. Pres. Antônio Carlos 6627, 31270-901 Belo Horizonte, MG, Brazil. isaccesar@bol.com.br
Andrade Nogueira
Fernando Henrique
FH
Antônio Pianetti
Gérson
G
D000962:Antimalarials / Q000032:analysis* / Q000737:chemistry; D000077549:Artemether; D037621:Artemisinins / Q000032:analysis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004304:Dosage Forms; D004338:Drug Combinations; D004983:Ethanolamines / Q000032:analysis* / Q000737:chemistry; D005449:Fluorenes / Q000032:analysis* / Q000737:chemistry; D000078102:Lumefantrine; D015394:Molecular Structure; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D013607:Tablets / Q000032:analysis
D000962:Antimalarials; D037621:Artemisinins; D004304:Dosage Forms; D004338:Drug Combinations; D004983:Ethanolamines; D005449:Fluorenes; D013607:Tablets; D000077549:Artemether; D000078102:Lumefantrine