[Effect of emodin on hepatocellular apoptosis following orthotopic liver transplantation in rats].
OBJECTIVE
To evaluate the effect of emodin on hepatocellular apoptosis following orthotopic liver transplantation (OLT) in rats.


METHODS
The LEW --> BN OLT models were established. A total of 24 rats were divided randomly and equally into 4 groups. Group A was treated with normal saline at dose of 0.5 mL x d(-1) intraperitoneally from 1st day to 8 th day after operation. Group B, CsA at dose of 10.0 mg x kg(-1) x d(-1). Group C, emodin at dose of 50.0 mg x kg(-1) x d(-1). Group D, CsA at dose of 10.0 mg x kg(-1) x d(-1) and emodin at dose of 50.0 mg x kg(-1) x d(-1). Fifteen days after operation, rejection active index (RAI) and hepatocellular apoptosis index (AI) was confirmed after observing the pathologic change of transplanted liver in recipients.


RESULTS
Respectively, the RAI of group A, B, C, D was 7.67 +/- 0.98, 5.17 +/- 0.40, 5.83 +/- 0.75, 3.83 +/- 0.75 and the AI of group A, B, C, D was 35.83 +/- 2.320, 15.83 +/- 1.33, 16.50 +/- 2.35, 11.50 +/- 1.05. The RAI and AI of group B, C, D was significantly lower than group A (P < 0.01) and group D was significantly lower than group B, C too (P < 0.05). There was no significant distinction between group B and C in RAI and AI.


CONCLUSIONS
Emodin has the effect of reduce the hepatocellular apoptosis following OLT in rats and the effect can stronger by CsA.
Lin
Sheng-Zhang
SZ
Department of Surgery, Second Affiliated Hospital, Wenzhou Medical College, Wenzhou 325003, China. wzf21sz@163.com
Xu
Xian-Chou
XC
Jing
He
H
Yang
Xiao
X
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D004642:Emodin / Q000494:pharmacology*; D006084:Graft Rejection; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects* / Q000276:immunology; D016031:Liver Transplantation; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew
D004642:Emodin