title: Mitosis-dependent protein expression in neuroblastoma cell line N1E-115.
abstract: Systematic work on differential protein expression in mitosis is limited, and we therefore used neuroblastoma cells (N1E-115) incubated with either colcemid or nocodazole to arrest mitosis. Proteins were identified by MALDI-TOF/TOF and nano-LC-ESI-MS/MS with subsequent quantification of spot volumes with specific software. Immunoblotting was used for verification of selected proteins. Levels of 10 individual proteins were increased and levels of 6 proteins were decreased concordantly by both treatments. These proteins were constituents of heat shock and chaperone, cytoskeleton, proteasomal, heterochromatin, and DNA replication signaling as well as housekeeping and metabolic systems. Identification of mitosis-dependent proteins is of importance for the interpretation of previous work and for designing future experiments.
authors.affiliation: Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Wahringer Gurtel 18-20, Vienna, Austria.
authors.forename: Amedeo A
authors.initials: AA
authors.lastname: Azizi
authors.forename: Sung-Ung
authors.initials: SU
authors.lastname: Kang
authors.forename: Angelika
authors.initials: A
authors.lastname: Freilinger
authors.forename: Mariella
authors.initials: M
authors.lastname: Gruber-Olipitz
authors.forename: Wei-Qiang
authors.initials: WQ
authors.lastname: Chen
authors.forename: Jae-Won
authors.initials: JW
authors.lastname: Yang
authors.forename: Markus
authors.initials: M
authors.lastname: Hengstschläger
authors.forename: Irene
authors.initials: I
authors.lastname: Slavc
authors.forename: Gert
authors.initials: G
authors.lastname: Lubec
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis; D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D004261:DNA Replication / Q000502:physiology; D004268:DNA-Binding Proteins; D003703:Demecolcine / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D051379:Mice; D008938:Mitosis / Q000187:drug effects / Q000502:physiology*; D018832:Molecular Chaperones / Q000096:biosynthesis; D009447:Neuroblastoma; D015739:Nocodazole / Q000494:pharmacology; D009687:Nuclear Proteins / Q000096:biosynthesis; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D016601:RNA-Binding Proteins; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D050257:Tubulin Modulators / Q000494:pharmacology
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009687:Nuclear Proteins; C095938:Pa2g4 protein, mouse; D011506:Proteins; D016601:RNA-Binding Proteins; D050257:Tubulin Modulators; D046988:Proteasome Endopeptidase Complex; D015739:Nocodazole; D003703:Demecolcine