Analysis of autophagic and necrotic cell death in Dictyostelium.
Non-apoptotic cell death types can be conveniently studied in Dictyostelium discoideum, an exceptionally favorable model not only because of its well-known genetic and experimental advantages, but also because in Dictyostelium there is no apoptosis machinery that could interfere with non-apoptotic cell death. We show here how to conveniently demonstrate, assess, and study these non-apoptotic cell death types. These can be generated by use of modifications of the monolayer technique of Rob Kay et al., and either wild-type HMX44A Dictyostelium cells, leading to autophagic cell death, or the corresponding atg1- autophagy gene mutant cells, leading to necrotic cell death. Methods to follow these non-apoptotic cell death types qualitatively and quantitatively will be reported.
Giusti
Corinne
C
Centre d'Immunologie INSERM-CNRS-Univ.Medit. de Marseille-Luminy, Marseille, France.
Kosta
Artemis
A
Lam
David
D
Tresse
Emilie
E
Luciani
Marie-Françoise
MF
Golstein
Pierre
P
D000818:Animals; D001343:Autophagy / Q000502:physiology*; D004023:Dictyostelium / Q000166:cytology* / Q000254:growth & development; D005434:Flow Cytometry; D008854:Microscopy, Electron; D008858:Microscopy, Phase-Contrast; D009336:Necrosis / Q000503:physiopathology*