The effects of intraperitoneal sildenafil administration on healing of left colonic anastomoses and intra-abdominal adhesion formation in the presence of intra-abdominal infection.
OBJECTIVE
The objective of this pilot study was to establish an animal model for intra-abdominal infection and to examine the effect of sildenafil on anastomotic healing of the left colon and intra-abdominal adhesion formation.


METHODS
Fourteen Winstar rats underwent colonic transsection and primary anastomosis after performing intra-abdominal infection. Rats were divided into two groups: Group 1 (n = 7): intra-abdominal infection, resection, and anastomoses; Group 2 (n = 7): intra-abdominal infection, resection, anastomoses, and sildenafil. Anastomotic bursting pressures, hydroxyproline levels, histopathologic grading, and abdominal adhesions were accessed on the postoperative Day 7.


RESULTS
Anastomotic healing was found to be improved in terms of a bursting pressure (P = 0.02). Histopathological examination revealed an increase in angiogenesis (P = 0.007). Moreover, intra-abdominal adhesions were significantly less in rats given sildenafil (P = 0.03).


CONCLUSIONS
Sildenafil may improve anastomotic healing of the left colon and diminishes peritoneal adhesion formation in the presense of abdominal infection.
Ayten
Refik
R
Department of General Surgery, Faculty of Medicine, Firat University, Elazig, Turkey. refikayten@yahoo.com
Cetinkaya
Ziya
Z
Girgin
Mustafa
M
Ozercan
Ibrahim
I
Ustundag
Bilal
B
Aygen
Erhan
E
D000714:Anastomosis, Surgical; D000818:Animals; D003106:Colon / Q000601:surgery*; D004195:Disease Models, Animal; D006909:Hydroxyproline / Q000378:metabolism; D007274:Injections, Intraperitoneal; D010538:Peritonitis / Q000378:metabolism / Q000601:surgery*; D010726:Phosphodiesterase Inhibitors / Q000008:administration & dosage*; D010865:Pilot Projects; D010879:Piperazines / Q000008:administration & dosage*; D011687:Purines / Q000008:administration & dosage; D051381:Rats; D017208:Rats, Wistar; D000068677:Sildenafil Citrate; D013450:Sulfones / Q000008:administration & dosage*; D000267:Tissue Adhesions / Q000209:etiology / Q000378:metabolism / Q000517:prevention & control; D014945:Wound Healing / Q000187:drug effects*
D010726:Phosphodiesterase Inhibitors; D010879:Piperazines; D011687:Purines; D013450:Sulfones; D000068677:Sildenafil Citrate; D006909:Hydroxyproline