Cyclin A probes by means of intermolecular sensitization of terbium-chelating peptides.
Intermolecular sensitization of lanthanide ions was effectively implemented in the development of fluorescent sensors targeting cyclin A. A chelating unit has been conjugated to peptides containing a known cyclin A binding motif (CBM). Upon interaction of the modified terbium-chelating peptides with the cyclin A substrate recruitment groove, the Tb3+ ion is placed in the vicinity of the Trp217 side chain, which results in efficient intermolecular terbium sensitization and specific long wavelength fluorescent emission from the metal center.
Pazos
Elena
E
Departamento de Química Orgánica, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Torrecilla
Daniel
D
Vázquez López
Miguel
M
Castedo
Luis
L
Mascareñas
José L
JL
Vidal
Anxo
A
Vázquez
M Eugenio
ME
D001665:Binding Sites; D015374:Biosensing Techniques / Q000379:methods; D002614:Chelating Agents / Q000737:chemistry; D018360:Crystallography, X-Ray; D019925:Cyclin A / Q000032:analysis* / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D008958:Models, Molecular; D009842:Oligopeptides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence; D013725:Terbium / Q000737:chemistry*
D002614:Chelating Agents; D019925:Cyclin A; D005456:Fluorescent Dyes; D006573:Heterocyclic Compounds, 1-Ring; D009842:Oligopeptides; D013725:Terbium; C071349:1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid