Intracoronary delivery of bone marrow cells to the acutely infarcted myocardium. Optimization of the delivery technique.
OBJECTIVE
Intracoronary cell transplantation during catheter balloon inflations may be associated with adverse events. We studied the effectiveness of an alternative transplantation technique--intracoronary cell infusion.


METHODS
Fourteen pigs, which had survived acute myocardial infarction, were randomized into 2 treatment groups and 2 controls. Three days after infarction, 12 pigs underwent allogeneic intracoronary mononuclear bone marrow cell transplantation using either the standard technique (short-term cell injections during repeat balloon inflations, technique A, n = 6) or continuous intracoronary cell infusion without balloon inflations (technique B, n = 6). Implanted cells were stained with fluorescent dye. After transplantation, the pigs were euthanized and myocardial samples were analyzed by fluorescent microscopy.


RESULTS
The mean numbers of fluorescently labeled bone marrow cells in the infarction border zone, in the infarction mid-area and in the center of myocardial infarction were 84, 72 and 55 using technique A, and 29, 57 and 46 using technique B, respectively. The mean cell retention in the infarction border zone of 84 cells for technique A and 29 cells for technique B differed significantly (p = 0.034, two-tailed t test).


CONCLUSIONS
The continuous intracoronary cell infusion technique is a less efficient cell delivery technique as compared with the standard technique using repeat intracoronary balloon inflations.
Meluzín
Jaroslav
J
1st Department of Internal Medicine/Cardioangiology, St. Anna Hospital, ICRC, Masaryk University, Pekarská 53, Brno, Czech Republic. jaroslav.meluzin@fnusa.cz
Vlasín
Michal
M
Groch
Ladislav
L
Mayer
Jirí
J
Kren
Leos
L
Rauser
Petr
P
Tichý
Boris
B
Hornácek
Ivan
I
Sitar
Jan
J
Palsa
Stanislav
S
Klabusay
Martin
M
Korístek
Zdenek
Z
Doubek
Michael
M
Pospísilová
Sárka
S
Lexmaulová
Leona
L
Dusek
Ladislav
L
D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D006328:Cardiac Catheterization; D002404:Catheterization; D004195:Disease Models, Animal; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence; D009203:Myocardial Infarction / Q000628:therapy*; D013552:Swine
D005452:Fluoresceins; D005456:Fluorescent Dyes; C069306:5-chloromethylfluorescein