title: ITPase activity in dry blood spots is comparable with that in fresh erythrocytes.
abstract: Inosine triphosphate pyrophosphohydrolase (ITPase) catalyzing the pyrophosphohydrolysis of inosine triphosphate, deoxyinosine triphosphate and xanthosine triphosphate is involved in the metabolism and tolerance of thiopurine drugs. ITPase activity plays an important role in the prediction of toxicity to thiopurine therapy. Activities in dry blood spots were compared with fresh erythrocytes. Samples were incubated with inosine triphosphate, then inosine monophosphate was determined by a capillary electrophoresis method. Calculated enzyme activities obtained from dry blood spots were in good accordance with activity in fresh erythrocytes.
authors.affiliation: Department of Clinical Biochemistry, Laboratory for Inherited Metabolic Disorders, Medical Hospital, Olomouc, Czech Republic. janatomkova@centrum.cz
authors.forename: J
authors.initials: J
authors.lastname: Tomkova
authors.forename: D
authors.initials: D
authors.lastname: Friedecky
authors.forename: P
authors.initials: P
authors.lastname: Vyskocilova
authors.forename: T
authors.initials: T
authors.lastname: Adam
mesh_terms: D003890:Desiccation; D004795:Enzyme Stability; D004912:Erythrocytes / Q000201:enzymology*; D006801:Humans; D011177:Postal Service; D011755:Pyrophosphatases / Q000032:analysis* / Q000378:metabolism*; D012044:Regression Analysis; D013048:Specimen Handling; D000097630:Inosine Triphosphatase
chemical_list: D011755:Pyrophosphatases; D000097630:Inosine Triphosphatase