Study on the interaction of porphyrin with G-quadruplex DNAs.
Interactions of 5,10,15,20-Tetrakis(N-propylpyridinium-4-yl)-21H,23H-porphyrin (TPrPyP4) with dimer hairpin (G4T4G4)2 and parallel four-stranded (TG4T)4 G-quadruplex DNAs in Na+-containing buffer were studied. The results show that two TPrPyP4 molecules bind to both G-quadruplexes by a noncooperative and nonequivalent binding mode, and there are one high affinity site and one low affinity site, the respective binding constants are 8.06 x 10(8) and 1.13 x 10(6) M(-1) for (G4T4G4)2-TPrPyP4, 8.04 x 10(7) and 9.08 x 10(5)M(-1) for (TG4T)4-TPrPyP4. TPrPyP4 presents two lifetimes of about 5.8 and 12.0 ns in the complexes of G-quadruplexes-TPrPyP4. The primary results suggest that two TPrPyP4 molecules bind to both G-quadruplexes by terminal stacking and outside binding mode.
Wei
Chunying
C
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. weichuny@sxu.edu.cn
Han
Gaoyi
G
Jia
Guoqing
G
Zhou
Jun
J
Li
Can
C
D004247:DNA / Q000737:chemistry*; D054856:G-Quadruplexes; D006147:Guanine / Q000737:chemistry*; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D011166:Porphyrins / Q000737:chemistry*
D011166:Porphyrins; D006147:Guanine; D004247:DNA