Metabolism and distribution of guanosine given intraperitoneally: implications for spinal cord injury.
Intraperitoneal administration of guanosine to rats with chronic spinal cord injury stimulates remyelination and functional recovery. If guanosine produced its effects in the nervous system, it should enter it and elevate endogenous concentrations. [(3)H]-guanosine (8 mg/kg) was administered intraperitoneally to rats and its distribution and concentration in different sites determined. Guanosine rapidly entered all tissues; its concentration peaked at about 15 minutes except in adipose tissue and CNS where it continued to rise for 30 minutes. Its chief metabolic product in all sites was guanine with over twice as much guanine as guanosine present in CNS after 30 minutes.
Jiang
Shucui
S
Department of Surgery, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada. jiangs@mcmaster.ca
Fischione
Gemma
G
Giuliani
Patricia
P
Guiliani
Patricia
P
Romano
Silvia
S
Caciagli
Francesco
F
Di Iorio
Patrizia
P
Diiorio
Patrizia
P
D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D005260:Female; D006147:Guanine / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics; D006151:Guanosine / Q000097:blood / Q000378:metabolism* / Q000493:pharmacokinetics* / Q000627:therapeutic use; D007274:Injections, Intraperitoneal; D051381:Rats; D013119:Spinal Cord Injuries / Q000188:drug therapy* / Q000378:metabolism*; D014018:Tissue Distribution; D019820:Xanthine / Q000097:blood / Q000378:metabolism / Q000493:pharmacokinetics
D006151:Guanosine; D019820:Xanthine; D006147:Guanine