Genotypes and clinical aspects associated with bone mineral density in Argentine postmenopausal women.
The aim of this study was to determine genotypes and clinical aspects associated with bone mineral density (BMD) in postmenopausal women from Córdoba, Argentina. Polymorphisms were assessed by RFLP-PCR technique using BsmI and FokI for vitamin D receptor gene (VDR) and XbaI and PvuII for estrogen receptor-alpha gene (ERalpha) as restrictases. Sixty-eight healthy, 54 osteopenic, and 64 osteoporotic postmenopausal women were recruited. Femoral neck and lumbar spine BMD were inversely correlated with age in the entire analyzed population. Height was lower in osteopenic and osteoporotic women as compared to healthy women (P < 0.05). Weight and body mass index (BMI) were the lowest in osteoporotic women (P < 0.01 versus healthy group). Serum procollagen type I Nterminal propeptide (PINP) was higher in osteoporotic women as compared to the other groups. Distribution of VDR and ERalpha genotypes was similar in the three groups. Genotype bb (VDR) was associated with low values of lumbar BMD in the healthy group (P < 0.05 versus genotype Bb), and with low values of femoral BMD (P < 0.05 versus genotype BB) in osteoporotic women. BB*Pp interaction was associated with the highest femoral neck BMD (P < 0.05), whereas the bb*xx interaction was associated with the lowest femoral neck BMD in the total population analyzed (P < 0.05). In conclusion, parameters such as age, height, weight, BMI, serum PINP, VDR genotypes, and interactions between VDR and ERalpha genotypes could be useful to predict a decrease in BMD in Argentine postmenopausal women.
Pérez
Adriana
A
Laboratorio de Metabolismo Fosfocálcico y Vitamina D Dr. Fernando Cañas, Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Cosquín 1673, Barrio Jardín, 5014 Córdoba, Argentina.
Ulla
María
M
García
Beatriz
B
Lavezzo
María
M
Elías
Eliana
E
Binci
Miriam
M
Rivoira
María
M
Centeno
Viviana
V
Alisio
Arturo
A
Tolosa de Talamoni
Nori
N
D001118:Argentina; D001827:Body Height; D015992:Body Mass Index; D001835:Body Weight; D015519:Bone Density / Q000235:genetics*; D005260:Female; D005272:Femur Neck / Q000502:physiology; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D008159:Lumbar Vertebrae / Q000502:physiology; D008875:Middle Aged; D010446:Peptide Fragments / Q000378:metabolism; D011110:Polymorphism, Genetic; D017698:Postmenopause / Q000235:genetics*; D011347:Procollagen / Q000378:metabolism; D018167:Receptors, Calcitriol / Q000235:genetics; D011960:Receptors, Estrogen / Q000235:genetics
D010446:Peptide Fragments; D011347:Procollagen; D018167:Receptors, Calcitriol; D011960:Receptors, Estrogen; C047202:procollagen Type I N-terminal peptide