title: IL-18 gene expression pattern in exogenously treated AML cells.
abstract: IL-18 production may enhance immune system defense against KG-1 cells ; NB4 cells, which are associated with good prognosis, do not produce IL-18. In this study, we treated KG-1 cells with IL-18 and used microarray technology to assess subsequent effects on gene expression. In UniGene-array of 7488 human genes, expression of 57 genes, including stress related genes, increased at least 2-fold, whereas expression of 48 genes decreased at least 2-fold. Following exogenous exposure of KG-1 cells to IL-18, expression of CRYGC, NF(kappa)BIA and NACA gene were monitored. The latter is a transcriptional coactivator potentiating c-Jun-mediated transcription. NF(kappa)BIA is an inhibitor of NF(kappa)B, and affects growth regulation, apoptosis and hypoxic stress. Studies, such as this one, are beginning to clarify the differences between cells associated with good and bad cancer prognoses, which may ultimately assist in medical treatment for acute myeloid leukemia.
authors.affiliation: Department of Biological Science, Sookmyung Woman's University, Seoul, Korea.
authors.forename: Minji
authors.initials: M
authors.lastname: Seo
authors.forename: Minha
authors.initials: M
authors.lastname: Park
authors.forename: Yeonjoo
authors.initials: Y
authors.lastname: Yook
authors.forename: Young Sook
authors.initials: YS
authors.lastname: Kwon
authors.forename: Young Ju
authors.initials: YJ
authors.lastname: Suh
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Kim
authors.forename: Daeho
authors.initials: D
authors.lastname: Cho
authors.forename: Jong Hoon
authors.initials: JH
authors.lastname: Park
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D020382:Interleukin-18 / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D020382:Interleukin-18; D016328:NF-kappa B