Quantitative toxoplasma gondii oocyst detection by a modified Kato Katz test using Kinyoun staining (KKK) in ME49 strain experimentally infected cats.
We detected Toxoplasma gondii oocysts in feces of experimentally infected cats, using a Kato Katz approach with subsequent Kinyoun staining. Animals serologically negative to T. gondii were infected orally with 5 x 10(2) mice brain cysts of ME49 strain. Feces were collected daily from the 3rd to the 30th day after challenge. Oocysts were detected by qualitative sugar flotation and the quantitative modified Kato Katz stained by Kinyoun (KKK). In the experimentally infected cats, oocysts were detected from the 7th to 15th day through sugar flotation technique, but oocysts were found in KKK from the 6th to 16th day, being sensitive for a larger period, with permanent documentation. The peak of oocysts excretion occurred between the 8th to 11th days after challenge, before any serological positive result. KKK could be used in the screening and quantification of oocysts excretion in feces of suspected animals, with reduced handling of infective material, decreasing the possibility of environmental and operator contamination.
Meireles
Luciana Regina
LR
Instituto de Medicina Tropical de São Paulo, Avenida Dr. Eneas de Carvalho Aguiar 470, São Paulo, SP, Brazil.
Tsutsui
Vinícius Suehiro
VS
Carmo
Claudia Villano do
CV
Galisteo
Andrés Jimenez
AJ
Hiramoto
Roberto Mitsuyoshi
RM
Terentowicz
Henrique César Katsumi
HC
Andrade
Heitor Franco de
HF
D000818:Animals; D002415:Cats; D005243:Feces / Q000469:parasitology*; D033661:Oocysts; D010270:Parasite Egg Count / Q000379:methods / Q000662:veterinary; D013194:Staining and Labeling / Q000379:methods / Q000662:veterinary; D013997:Time Factors; D014122:Toxoplasma / Q000302:isolation & purification*; D014124:Toxoplasmosis, Animal / Q000469:parasitology*