Attenuating effect of daidzein on polychlorinated biphenyls-induced oxidative toxicity in mouse testicular cells.
The attenuating effect of daidzein (DAI) on oxidative toxicity induced by Aroclor 1254 (A1254) was investigated in mouse testicular cells. Cells were exposed to A1254 alone or with DAI. The oxidative damage was estimated by measuring malondialdehyde (MDA) formation, superoxide dismutase (SOD) activity and glutathione (GSH) content. Results show that A1254 induced a decrease of germ cell number, an elevation in thiobarbituric acid reactive substances (TBARS) but a decrease in SOD activity and GSH content. However, simultaneous supplementation with DAI decreased TBARS level and increased SOD activity and GSH content. Consequently, dietary DAI may restore the intracellular antioxidant system to attenuate the oxidative toxicity of A1254 in testicular cells.
Zhang
Da-Lei
DL
College of Animal Sciences, Zhejiang University, Hangzhou, China.
Mi
Yu-Ling
YL
Wang
Kai-Ming
KM
Zeng
Wei-Dong
WD
Zhang
Cai-Qiao
CQ
D000818:Animals; D020111:Chlorodiphenyl (54% Chlorine) / Q000633:toxicity*; D019271:Hypoxanthine / Q000633:toxicity; D007529:Isoflavones / Q000494:pharmacology*; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008813:Mice, Inbred ICR; D010084:Oxidation-Reduction; D013737:Testis / Q000187:drug effects* / Q000378:metabolism; D014969:Xanthine Oxidase / Q000633:toxicity
D007529:Isoflavones; D020111:Chlorodiphenyl (54% Chlorine); D019271:Hypoxanthine; D008315:Malondialdehyde; C004742:daidzein; D014969:Xanthine Oxidase