title: Displacement effects in large-scale chromatography?
abstract: Displacement effects in large-scale (total column volume v(t) = 150 L) and preparative ion-exchange chromatography purifying human erythrocyte superoxide dismutase are described in the present article. The biomolecules are eluted in a very small peak elution volume (<0.2 v(o)) behind the salt wave using a step gradient. The theoretical peak width and retention behavior are calculated according to the model of Yamamoto. The theoretical values are then compared with the experimental data. There was a difference observed between the elution type I (also called fronting) and the experimentally obtained elution. Some instructions are given on how to achieve these phenomenona because a beneficial effect in respect to resolution and recovery of a biomolecule is observed.
authors.affiliation: Institute of Applied Microbiology, University of Bodenkultur, Nussdorferlaende 11 82, A-1190 Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Jungbauer
authors.forename: K
authors.initials: K
authors.lastname: Uhl
authors.forename: P
authors.initials: P
authors.lastname: Schulz
authors.forename: C
authors.initials: C
authors.lastname: Tauer
authors.forename: G
authors.initials: G
authors.lastname: Gruber
authors.forename: F
authors.initials: F
authors.lastname: Steindl
authors.forename: A
authors.initials: A
authors.lastname: Buchacher
authors.forename: W
authors.initials: W
authors.lastname: Schoenhofer
authors.forename: F
authors.initials: F
authors.lastname: Unterluggauer