Relationship of Porphyromonas gingivalis with glycemic level in patients with type 2 diabetes following periodontal treatment.
BACKGROUND
The aim of this study was to assess the relationship between serum glycemic levels and subgingival microbial profile alteration following periodontal treatment in patients with type 2 diabetes mellitus.


METHODS
We studied 30 periodontitis patients with type 2 diabetes mellitus who received full-mouth subgingival debridement by analyzing their subgingival microbial profiles using a polymerase chain reaction method at baseline and various time-points for 12 months following treatment. Concurrently, probing pocket depth, bleeding on probing, and metabolic parameters, including glycated hemoglobin A1c (HbA1c), blood sugar level, C-reactive proteins, total cholesterol, triglyceride, and high-density and low-density lipoprotein cholesterol, were recorded.


RESULTS
Periodontal conditions were significantly improved after treatment, and the occurrence rates of periodontal bacterial species, including Porphyromonas gingivalis, Tannerella forsythensis, Treponema denticola, and Prevotella intermedia, were also reduced. Interestingly, P. gingivalis was detected more frequently in subjects with increased HbA1c values after periodontal treatment than in those patients with decreased HbA1c values. Furthermore, P. gingivalis with type II fimbriae was detected only in HbA1c-increased subjects, while improvements in HbA1c values were observed only in subjects without type II clones.


CONCLUSIONS
These results suggest that glycemic level in diabetes is affected by the persistence of P. gingivalis, especially clones with type II fimbriae, in periodontal pockets.
Makiura
N
N
Department of Oral Frontier Biology, Osaka University Graduate School of Dentistry, Suita-Osaka 565-0871, Japan.
Ojima
M
M
Kou
Y
Y
Furuta
N
N
Okahashi
N
N
Shizukuishi
S
S
Amano
A
A
D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016976:Aggregatibacter actinomycetemcomitans / Q000302:isolation & purification; D001439:Bacteroides / Q000302:isolation & purification; D001786:Blood Glucose / Q000032:analysis*; D002097:C-Reactive Protein / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015169:Colony Count, Microbial; D003924:Diabetes Mellitus, Type 2 / Q000097:blood*; D005260:Female; D010861:Fimbriae, Bacterial / Q000145:classification; D005500:Follow-Up Studies; D005884:Gingival Hemorrhage / Q000628:therapy; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010514:Periodontal Pocket / Q000628:therapy; D010518:Periodontitis / Q000382:microbiology / Q000628:therapy*; D016966:Porphyromonas gingivalis / Q000302:isolation & purification*; D013357:Subgingival Curettage; D048373:Treponema denticola / Q000302:isolation & purification; D014280:Triglycerides / Q000097:blood
D001786:Blood Glucose; D002097:C-Reactive Protein; D002784:Cholesterol; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D006442:Glycated Hemoglobin; D014280:Triglycerides