title: The brain proteome profile is highly conserved between Prnp-/- and Prnp+/+ mice.
abstract: The aim of this study is to compare the proteome of Prnp-/- (Zürich I) gene-ablated mouse brains with wild-type mouse brains. Fluorescence two-dimensional-difference gel electrophoresis (DIGE) and isotope-coded protein labeling (ICPL) were applied for brain homogenates. Similar quantitative protein profiles (<or=1.5-fold change, P<0.05) were obtained by quantitative two-dimensional-DIGE analysis of whole brain homogenates of two developmental stages (days 1 and 67). Complementary ICPL-liquid chromatography-mass spectrometry experiments (n=5) of whole brain homogenates and synaptosomes were performed. The abundance ratios of all proteins quantified did not exceed the statistical spread determined (<2-fold). Therefore, both of the two-dimensional-DIGE and ICPL analyses demonstrated a highly conserved quantitative protein profile in brain homogenates of Prnp-/- and Prnp+/+ mice.
authors.affiliation: Laboratory of Molecular Neuropathology, Centre of Neuropathology and Prion Research, LAFUGA, Gene Centre, LMU, Munich, Germany.
authors.forename: Anna C
authors.initials: AC
authors.lastname: Crecelius
authors.forename: Derrick
authors.initials: D
authors.lastname: Helmstetter
authors.forename: Julia
authors.initials: J
authors.lastname: Strangmann
authors.forename: Gerda
authors.initials: G
authors.lastname: Mitteregger
authors.forename: Thomas
authors.initials: T
authors.lastname: Fröhlich
authors.forename: Georg J
authors.initials: GJ
authors.lastname: Arnold
authors.forename: Hans A
authors.initials: HA
authors.lastname: Kretzschmar
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002853:Chromatography, Liquid / Q000379:methods; D030562:Databases, Protein; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D000072002:Prion Proteins; D011328:Prions / Q000235:genetics*; D020543:Proteome / Q000378:metabolism*
chemical_list: D009419:Nerve Tissue Proteins; D000072002:Prion Proteins; D011328:Prions; C512657:Prnp protein, mouse; D020543:Proteome