[Study of expired air plateau period by oral administration of 13C-leucine during feeding state].
OBJECTIVE
To explore the expired air plateau period by oral administration of 13C-Leucine during feeding state in study of amino acid metabolism kinetics.


METHODS
Six healthy adult women were selected as subjects, 13C-leucine was orally administrated for 4 hours during feeding state on day 6 after 5 days of adaptation period. The expired air was collected during the course of administration and 1 hour after the end of administration to determine the delta over baseline for 13C.


RESULTS
The delta over baseline for 13C in expired air was steady at a higher level during period of half hour before and after the end of administration respectively.


CONCLUSIONS
It was suggested that half hour before and after the end of administration respectively was the optimal period for sampling in the study of metabolism kinetics of amino acid by oral administration of 13C-leucine during feeding state.
Tian
Ying
Y
Laboratory of Trace Element Nutrition MOH, Institute of Nutrition and Food Safety, China CDC, Beijing 100050, China.
Piao
Jianhua
J
Gou
Lingyan
L
Tian
Yuan
Y
et al
D000596:Amino Acids / Q000378:metabolism*; D001944:Breath Tests / Q000379:methods*; D002245:Carbon Dioxide / Q000032:analysis; D002247:Carbon Isotopes / Q000032:analysis* / Q000378:metabolism; D005260:Female; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D007700:Kinetics; D007930:Leucine / Q000008:administration & dosage* / Q000378:metabolism; D013997:Time Factors; D055815:Young Adult
D000596:Amino Acids; D002247:Carbon Isotopes; D002245:Carbon Dioxide; D007930:Leucine