Congenital disease SNPs target lineage specific structural elements in protein kinases.
The catalytic domain of protein kinases harbors a large number of disease-causing single nucleotide polymorphisms (SNPs) and common or neutral SNPs that are not known or hypothesized to be associated with any disease. Distinguishing these two types of polymorphisms is critical in accurately predicting the causative role of SNPs in both candidate gene and genome-wide association studies. In this study, we have analyzed the structural location of common and disease-associated SNPs in the catalytic domain of protein kinases and find that, although common SNPs are randomly distributed within the catalytic core, known disease SNPs consistently map to regulatory and substrate binding regions. In particular, a buried side-chain network that anchors the flexible activation loop to the catalytic core is frequently mutated in disease patients. This network was recently shown to be absent in distantly related eukaryotic-like kinases, which lack an exaggerated activation loop and, presumably, are not regulated by phosphorylation.
Torkamani
Ali
A
Graduate Program in Biomedical Sciences, University of California, San Diego, CA 92103, USA.
Kannan
Natarajan
N
Taylor
Susan S
SS
Schork
Nicholas J
NJ
D000494:Allosteric Regulation; D020134:Catalytic Domain / Q000235:genetics; D000013:Congenital Abnormalities / Q000235:genetics*; D017124:Conserved Sequence; D019143:Evolution, Molecular; D006801:Humans; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011494:Protein Kinases / Q000737:chemistry*; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary; D013379:Substrate Specificity
D011494:Protein Kinases