In situ recalibration of probes of dissolved gas concentrations in bioreactors without effect on metabolism and growth.
This article describes a general method for the in situ recalibration of a probe for dissolved gas concentrations without changing any parameter but the gas composition. Recalibration of an oxygen probe is used as an example.The recalibration procedure can be used for any probe, provided the culture's rate of metabolism and growth is independent of the substrate concentration in the range being measured by the probe during recalibration. The method was tested using a galvanic oxygen electrode in an oxygen-consuming culture of Klebsiella oxytoca and an oxygen-evolving culture of Anabaena variabilis. The recalibration caused no detectable effect on the metabolism and growth of the cultures, provided the dissolved oxygen tension was above 3 microM.
Iversen
J J
JJ
Institute of Biochemistry, Odense University, DK-5230 Odense M, Denmark.