Lysophosphatidic acid can support the formation of membranous structures and an increase in MBP mRNA levels in differentiating oligodendrocytes.
During development, differentiating oligodendrocytes progress in distinct maturation steps from premyelinating to myelinating cells. Such maturing oligodendrocytes express both the receptors mediating signaling via extracellular lysophosphatidic acid (LPA) and the major enzyme generating extracellular LPA, namely phosphodiesterase-Ialpha/autotaxin (PD-Ialpha/ATX). However, the biological role of extracellular LPA during the maturation of differentiating oligodendrocytes is currently unclear. Here, we demonstrate that application of exogenous LPA induced an increase in the area occupied by the oligodendrocytes' process network, but only when PD-Ialpha/ATX expression was down-regulated. This increase in network area was caused primarily by the formation of membranous structures. In addition, LPA increased the number of cells positive for myelin basic protein (MBP). This effect was associated by an increase in the mRNA levels coding for MBP but not myelin oligodendrocyte glycoprotein (MOG). Taken together, these data suggest that LPA may play a crucial role in regulating the later stages of oligodendrocyte maturation.
Nogaroli
Luciana
L
Department of Anatomy and Neurobiology, Virginia Commonwealth University School of Medicine, PO Box 980709, Richmond, VA 23298, USA.
Yuelling
Larra M
LM
Dennis
Jameel
J
Gorse
Karen
K
Payne
Shawn G
SG
Fuss
Babette
B
D000818:Animals; D002454:Cell Differentiation; D005260:Female; D008246:Lysophospholipids / Q000502:physiology*; D004676:Myelin Basic Protein / Q000235:genetics*; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein / Q000096:biosynthesis; D063308:Myelin-Oligodendrocyte Glycoprotein; D009836:Oligodendroglia / Q000502:physiology*; D010727:Phosphoric Diester Hydrolases / Q000096:biosynthesis; D011755:Pyrophosphatases / Q000096:biosynthesis; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D049368:Receptors, Lysophosphatidic Acid / Q000096:biosynthesis; D000099189:Lysophospholipase D
D008246:Lysophospholipids; D004676:Myelin Basic Protein; D009185:Myelin Proteins; D018992:Myelin-Associated Glycoprotein; D063308:Myelin-Oligodendrocyte Glycoprotein; D010727:Phosphoric Diester Hydrolases; D011755:Pyrophosphatases; D012333:RNA, Messenger; D049368:Receptors, Lysophosphatidic Acid; D000099189:Lysophospholipase D; C571328:Mog protein, rat; C032881:lysophosphatidic acid