The large-scale immobilization of Penicillium chrysogenum: batch and continuous operation in an air-lift reactor.
A temperature-sensitive cell division cycle mutant of Penicillium chrysogenum P2 has been immobilized on Celite and grown in a 250-320-L working volume air-lift fermenter. The ability to uncouple growth and penicillin synthesis by raising the temperature to 30 degrees C also overcame the problem of the free cell mass which appeared after 300 h operation with the parent organism. After 500 h operation, penicillin and ACV dimer were still being synthesized.
Keshavarz
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SERC Centre for Biochemical Engineering, Department of Chemical and Biòchemical Engineering, University College London, Torrington Place, Loñdon WC1E 7JE, United Kingdom.
Eglin
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Walker
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Bucke
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Holt
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Bull
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Lilly
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MD