In vitro culture of hybridoma cells in agarose beads producing antibody secretion for two weeks.
A new process for embedding cells in agarose is described. Beads were obtained by extruding an ultralow gelling temperature agarose solution in a capillary containing a hydrophobic medium flowthrough. The toxicity of the procedure has been evaluated by monitoring the energy status of agarose-embedded C(6) glioma cells with (31)P nuclear magnetic resonance (NMR). Suspension and microbead cultures of hybridoma cell line were compared. In suspension culture the number of cells and the antibody concentrations increased for 5 days before the stationary phase began, when the cultures were stopped. In agarose bead cultures, the gel provided an enormous support surface area (50 m(2)/ mL of gel). It was possible to seed 20-fold more cells. The gel pressure modified the proliferative process and antibody pattern secretion. In particular, the antibodies could be harvested for two weeks.
Cadic
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C
INSERM-U. 306, Université de Bordeaux II, 33076 Bordeaux Cedex, France.
Dupuy
B
B
Pianet
I
I
Merle
M
M
Margerin
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C
Bezian
J H
JH