[Biocompatibility of a novel biological piezoelectric ceramic to the rat periosteum derived osteoblast].
OBJECTIVE
A novel biological piezoelectric ceramic was made by beta-tricalcium phosphate (beta-TCP) and lithium sodium potassium niobate (LNK) piezoelectric ceramics. To study its biocompatibility to osteoblast isolated from the cranium of 1-day-old Sprague-Dawley mice.


METHODS
The biological piezoelectric ceramic TCPLNK1/10, TCPLNK5/5 respectively mixed by beta-TCP and LNK piezoelectric ceramic at the ratio of 1/10 and 5/5. Then osteoblasts were used and seeded respectively on the negative and positive surfaces of TCPLNK1/10 and TCPLNK5/5. Growth and proliferation of the osteoblasts on TCPLNK1/10 and TCPLNK5/5 surfaces were evaluated in vitro by means of scanning electron microscopy (SEM) examination, fluorescence dyeing of osteoblast skeleton protein and MTT assay.


RESULTS
Cell morphology of osteoblast on positive and negative surfaces of TCPLNK1/10 and TCPLNK5/5 was normal, and both adhesion and growth characteristics showed better than control group. The growing osteoblasts on the TCPLNK1/10 negative surface were significantly higher than others. The negative surface of TCPLNK1/10 possessed better osteogenesis potential than others in vitro.


CONCLUSIONS
The surface of TCPLNK may permit the imitation piezoelectric effect of natural bone for bone regeneration.
Wang
Peng
P
Department of Stomatology, Affiliated Hospital of Hebei University, Baoding 071000, China.
Zhang
Xiao-Zhou
XZ
Yang
Ping
P
Tian
Bao-Min
BM
Liu
Zhi-Liang
ZL
Chen
Zhi-Qing
ZQ
D000818:Animals; D001861:Bone Regeneration; D001842:Bone and Bones; D002130:Calcium Phosphates; D049109:Cell Proliferation; D002516:Ceramics; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009556:Niobium; D010006:Osteoblasts; D010012:Osteogenesis; D010087:Oxides; D010521:Periosteum; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull
D002130:Calcium Phosphates; D010087:Oxides; C485817:beta-tricalcium phosphate; D009556:Niobium; C091692:lithium niobate