Crystallization and preliminary X-ray analysis of a class II release factor RF3 from a sulfate-reducing bacterium.
Class II release factor 3 (RF3) from the sulfate-reducing bacterium Desulfovibrio vulgaris Miyazaki F, which promotes rapid dissociation of a class I release factor, has been overexpressed, purified and crystallized in complex with GDP at 293 K using the sitting-drop vapour-diffusion method. A data set was collected to 1.8 A resolution from a single crystal at 100 K using synchrotron radiation. The crystal belongs to space group P1, with unit-cell parameters a = 47.39, b = 82.80, c = 148.29 A, alpha = 104.21, beta = 89.78, gamma = 89.63 degrees . The asymmetric unit contains four molecules of the RF3-GDP complex. The Matthews coefficient was calculated to be 2.3 A(3) Da(-1) and the solvent content was estimated to be 46.6%.
Kihira
Kiyohito
K
Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
Numata
Shuko
S
Kitamura
Masaya
M
Kondo
Jun
J
Terawaki
Shinichi
S
Shomura
Yasuhito
Y
Komori
Hirofumi
H
Shibata
Naoki
N
Higuchi
Yoshiki
Y
D001426:Bacterial Proteins / Q000737:chemistry* / Q000145:classification* / Q000235:genetics; D003460:Crystallization; D016969:Desulfovibrio vulgaris / Q000737:chemistry* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D010454:Peptide Termination Factors / Q000737:chemistry* / Q000145:classification* / Q000235:genetics; D013431:Sulfates / Q000737:chemistry* / Q000378:metabolism; D014961:X-Ray Diffraction
D001426:Bacterial Proteins; D010454:Peptide Termination Factors; D013431:Sulfates; C087971:peptide-chain-release factor 3