Schisandrin B enhances cerebral mitochondrial antioxidant status and structural integrity, and protects against cerebral ischemia/reperfusion injury in rats.
Schisandrin B (Sch B), a dibenzocyclooctadiene derivative isolated from the fruit of Schisandra chinensis, has been shown to enhance mitochondrial antioxidant status in liver, heart and brain tissues in rodents. Whether or not long-term Sch B treatment can protect against oxidative stress-induced cerebral damage remains unclear. In the present study, the effect of long-term Sch B treatment (1-30 mg/kg/dx15) on cerebral ischemia/reperfusion (I/R) injury was examined in rats. Sch B treatment protected against I/R-induced cerebral damage, as evidenced by the significant increase in the percentage of 2,3,5-triphenyl tetrazolium chloride (TTC)-stained tissues in representative brain slices, when compared with the Sch B-untreated and I/R control. The cerebroprotection was associated with an enhancement in cerebral mitochondrial antioxidant status, as assessed by the level/activity of reduced glutathione, alpha-tocopherol and Mn-superoxide dismutase, as well as the improvement/preservation of mitochondrial structural integrity, as assessed by the extents of malondialdehyde production, Ca(2+) loading and cytochrome c release, as well as the sensitivity to Ca(2+)-induced permeability transition, in control and I/R-challenged rats. In conclusion, long-term Sch B treatment could enhance cerebral mitochondrial antioxidant status as well as improve mitochondrial structural integrity, thereby protecting against I/R injury.
Chen
Na
N
Department of Biochemistry, The Hong Kong University of Science and Technology, Hong Kong, SAR, China.
Chiu
Po Yee
PY
Ko
Kam Ming
KM
D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D019540:Area Under Curve; D002545:Brain Ischemia / Q000473:pathology / Q000517:prevention & control*; D034242:Cyclooctanes / Q000494:pharmacology; D005260:Female; D005638:Fruit / Q000737:chemistry; D005978:Glutathione / Q000378:metabolism; D017705:Lignans / Q000494:pharmacology*; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D011083:Polycyclic Compounds / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D032141:Schisandra / Q000737:chemistry; D013482:Superoxide Dismutase / Q000378:metabolism; D024502:alpha-Tocopherol / Q000378:metabolism
D000975:Antioxidants; D034242:Cyclooctanes; D017705:Lignans; D011083:Polycyclic Compounds; C015499:schizandrin B; D008315:Malondialdehyde; D013482:Superoxide Dismutase; D005978:Glutathione; D024502:alpha-Tocopherol