Polypropylene grafted with smart polymers (PNIPAAm/PAAc) for loading and controlled release of vancomycin.
New smart surface-modified polypropylene (PP) was prepared for improving the loading and the sustained delivery of vancomycin and, thus, reducing the risk of biofilm formation when used as component of biomedical devices. Isothermal titration calorimetry (ITC) served for screening the most suitable monomers for grafting; the drug preferentially bonding to ionized acrylic acid (AAc). A net-PP-g-PNIPAAm-inter-net-PAAc was synthesized by first grafting and cross-linking of N-isopropylacrylamide onto PP films and then interpenetrating a second network by redox polymerization and cross-linking of AAc. PP-g-PAAc slabs were prepared by grafting AAc and, optionally, cross-linking. The amount and composition of grafted polymer (FTIR-ATR), morphology (SEM), temperature- and pH-responsiveness (swelling measurements), thermal behavior (DSC), friction coefficient (rheometry), drug loading and release rate, and effect against methicillin-resistant Staphylococcus aureus (MRSA) biofilms (modified robbins device) were evaluated. Grafting of AAc notably decreased the friction coefficient from 0.28+/-0.03 to 0.05+/-0.02 and enhanced the vancomycin loading (up to 2.5mg/cm(2)). Drug-loaded films showed a pH-dependent release rate, sustaining the release in pH 7.4 aqueous media at 37 degrees C for several hours. All drug-loaded films reduced biofilm formation by MRSA; the anti-biofilm effect being statistically significant (91.7% reduction, alpha<0.05) for PP-g-PAAc with the thinnest grafting layer.
Ruiz
Juan-Carlos
JC
Departamento de Química de Radiaciones y Radioquímica, Universidad Nacional Autónoma de México, México DF, Mexico.
Alvarez-Lorenzo
Carmen
C
Taboada
Pablo
P
Burillo
Guillermina
G
Bucio
Emilio
E
De Prijck
Kristof
K
Nelis
Hans J
HJ
Coenye
Tom
T
Concheiro
Angel
A
D000178:Acrylamides / Q000737:chemistry*; D000180:Acrylic Resins / Q000737:chemistry*; D018441:Biofilms / Q000187:drug effects; D003692:Delayed-Action Preparations; D006863:Hydrogen-Ion Concentration; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects; D011108:Polymers / Q000737:chemistry*; D011126:Polypropylenes / Q000737:chemistry*; D014640:Vancomycin / Q000008:administration & dosage* / Q000737:chemistry
D000178:Acrylamides; D000180:Acrylic Resins; D003692:Delayed-Action Preparations; D011108:Polymers; D011126:Polypropylenes; C052970:poly-N-isopropylacrylamide; C006903:carbopol 940; D014640:Vancomycin