Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818).
The single cell gel electrophoresis or the comet assay was established in the freshwater snail Biomphalaria glabrata. For detecting DNA damage in circulating hemocytes, adult snails were irradiated with single doses of 2.5, 5, 10 and 20 Gy of (60)Co gamma radiation. Genotoxic effect of ionizing radiation was detected at all doses as a dose-related increase in DNA migration. Comet assay in B. glabrata demonstrated to be a simple, fast and reliable tool in the evaluation of genotoxic effects of environmental mutagens.
Grazeffe
Vanessa Siqueira
VS
Laboratório de Parasitologia/Malacologia, Instituto Butantan, Avenida Vital Brasil, 1500, CEP 05503-900, São Paulo, Brazil.
Tallarico
Lenita de Freitas
Lde F
Pinheiro
Alessandro de Sá
Ade S
Kawano
Toshie
T
Suzuki
Miriam Fussae
MF
Okazaki
Kayo
K
Pereira
Carlos Alberto de Bragança
CA
Nakano
Eliana
E
D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001700:Biomphalaria / Q000235:genetics / Q000528:radiation effects; D020552:Comet Assay; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004784:Environmental Monitoring / Q000379:methods*; D005720:Gamma Rays; D006458:Hemolymph / Q000528:radiation effects; D012680:Sensitivity and Specificity