ZO-1- and ZO-2-dependent integration of myosin-2 to epithelial zonula adherens.
For the zonula adherens (ZA) to be established by linear arrangement of adherens junctions (AJs) in epithelial sheet cells, critical for the epithelial cell sheet formation and intercellular barrier function, myosin-2 is supposedly integrated into the ZA with the result of overlapping localization of E-cadherin/actin/myosin-2. Here, we immunofluorescently showed that myosin-2 failed to be integrated into the ZA in cultured epithelial-type ZO1(ko)/2(kd) Eph4 cells lacking ZO-1 and -2 (zonula occludens-1 and -2) by knockout and knockdown, respectively. Instead, a linearized but fragmented arrangement of AJs was formed in the way that it was positive for E-cadherin/actin, but negative for myosin-2 (designated prezonula-AJ). Transfection of full-length ZO-1 or ZO-2, or ZO-1 lacking its PDZ (PSD-95/discs large/zonula occludens-1)-1/2 domains (but not one lacking PDZ-1/2/3) into ZO1(ko)/2(kd) Eph4 cells restored the junctional integration of myosin-2 with prezonula-AJ to establish the ZA. Transfection of dominant-active RhoA or Rho-kinase (ROCK), as well as administration of lysophosphatidic acid or Y27632, which activates RhoA or inhibits ROCK, respectively, suggested that RhoA regulated the junctional integration of myosin-2 into ZA in a manner such that ROCK played a necessary but not-sufficient role. Fluorescence resonance energy transfer analyses revealed that spatiotemporal Rho-activation occurred in a ZO-1/2-dependent way to establish ZA from primordial forms in epithelial cells.
Yamazaki
Yuji
Y
Laboratory of Biological Science, Graduate School of Frontier Biosciences, Osaka University, Suita, Osaka 565-0871, Japan.
Umeda
Kazuaki
K
Wada
Masami
M
Nada
Shigeyuki
S
Okada
Masato
M
Tsukita
Shoichiro
S
Tsukita
Sachiko
S
D000199:Actins / Q000378:metabolism; D022005:Adherens Junctions / Q000378:metabolism*; D000577:Amides / Q000494:pharmacology; D000818:Animals; D015820:Cadherins / Q000378:metabolism; D004847:Epithelial Cells; D004848:Epithelium / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D005786:Gene Expression Regulation; D008565:Membrane Proteins / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D009218:Myosins / Q000378:metabolism*; D010750:Phosphoproteins / Q000378:metabolism*; D011725:Pyridines / Q000494:pharmacology; D051381:Rats; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein
D000199:Actins; D000577:Amides; D015820:Cadherins; D008565:Membrane Proteins; D010750:Phosphoproteins; D011725:Pyridines; C569043:Tjp1 protein, mouse; C569044:Tjp1 protein, rat; C569047:Tjp2 protein, mouse; D062826:Zonula Occludens-1 Protein; D062827:Zonula Occludens-2 Protein; C108830:Y 27632; D009218:Myosins