Validation of a new method using the reactivity of electrogenerated superoxide radical in the antioxidant capacity determination of flavonoids.
This article lays out a new method to measure the antioxidant capacity of some flavonoids. The methodology developed is based on the kinetics of the reaction of the antioxidant substrate with the superoxide radical (O(2)(*-)). A cyclic voltammetric technique was used to generate O(2)(*-) by reduction of molecular oxygen in aprotic media. In the same experiment the consumption of the radical was directly measured by the anodic current decay of the superoxide radical oxidation in the presence of increasing concentrations of antioxidant substrate. The method was statistically validated on flavonoid monomers and on the standard antioxidants: trolox, ascorbic acid and phloroglucinol. The linear correlations between the anodic current of O(2)(*-) and the substrate concentration allowed the determination of antioxidant index values expressed by the substrate concentration needed to consume 30% (AI(30)) and 50% (AI(50)) of O(2)(*-) in given conditions of oxygen concentration and scanning rate. The fidelity of the method was examined intraday and interlaboratories.
Le Bourvellec
Carine
C
Laboratoire de Chimie Analytique URU-337, Faculté de Pharmacie, Université de Rennes 1, Avenue du Professeur Léon Bernard, 35043 Rennes Cedex, France.
Hauchard
Didier
D
Darchen
André
A
Burgot
Jean-Louis
JL
Abasq
Marie-Laurence
ML
D000975:Antioxidants / Q000737:chemistry*; D004563:Electrochemistry; D005419:Flavonoids / Q000032:analysis* / Q000737:chemistry*; D016014:Linear Models; D010100:Oxygen / Q000737:chemistry; D013481:Superoxides / Q000737:chemistry*
D000975:Antioxidants; D005419:Flavonoids; D013481:Superoxides; D010100:Oxygen