Physical localization of the retrotransposons Boudicca and Perere 03 in Schistosoma mansoni.
Schistosoma mansoni is 1 of the causative agents of schistosomiasis, an endemic disease in 76 countries of the world. The study of its genome, estimated to be 270 Mb, is very important to understanding schistosome biology, the mechanisms of drug resistance, and immune evasion. Repetitive elements constitute more than 40% of the S. mansoni genome and may play a role in the parasite evolution. The retrotransposons Boudicca, a long terminal repeat (LTR), and Perere 03, a non-LTR, are present in a high number in the S. mansoni genome and were localized with the use of fluorescence in situ hybridization (FISH) and primed in situ labeling (PRINS). Bacterial artificial chromosomes (BAC) clones containing the retrotransposons Boudicca and Perere 03 were selected by bioinformatic analysis and used as probes in FISH. Using metaphase chromosomes from sporocysts and the FISH and PRINS techniques, we were able to map these retrotransposons. Perere 03 was localized in the euchromatic regions of the short arm of chromosome 2 and Boudicca in the euchromatic regions of the short arm of chromosomes 2 and Z.
Valentim
C L L
CL
Departamento de Ciências Biológicas e Núcleo de Pesquisas em Ciências Biológicas (NUPEB) Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.
Gomes
M S
MS
Jeremias
W J
WJ
Cunha
J C
JC
Oliveira
G C
GC
Botelho
A C C
AC
Pimenta
P F P
PF
Janotti-Passos
L K
LK
Guerra-Sá
R
R
Babá
E H
EH
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics; D049751:Genome, Helminth / Q000235:genetics*; D017404:In Situ Hybridization, Fluorescence; D007621:Karyotyping; D018613:Microscopy, Confocal; D020401:Primed In Situ Labeling; D018626:Retroelements / Q000502:physiology*; D012550:Schistosoma mansoni / Q000235:genetics*; D016415:Sequence Alignment; D020079:Terminal Repeat Sequences
D018626:Retroelements