title: Plasma-assisted immobilization of heparin onto low-density polyethylene surface.
abstract: In this study heparin was covalently immobilized onto LDPE-VEMAC sheet fabricated by the introduction of carboxyl groups to the surface of low-density polyethylene (LDPE) using a plasma technique. The plasma irradiation time influenced the density of carboxyl groups on the LDPE-VEMAC sheet. Heparin was immobilized on the LDPE-VEMAC sheet using a condensation reagent, N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC). We confirmed the immobilization of heparin from the ATR-FT-IR spectrum of the sheet obtained. Where heparin was directly immobilized on the LDPE-VEMAC sheet, the density of the immobilized heparin depended on that of the carboxyl groups. Heparin was also immobilized with a spacer, hexamethylene diamine, and the density of such heparin was about 1.6 times that of the directly immobilized heparin. This result suggests that the introduction of a spacer may be an effective way to increase the density of immobilized heparin.
authors.affiliation: Laboratory of Pharmaceutical Physical Chemistry, Gifu Pharmaceutical University, Gifu, Japan. skondo@gifu-pu.ac.jp
authors.forename: Shin-Ichi
authors.initials: S
authors.lastname: Kondo
authors.forename: Yasuyo
authors.initials: Y
authors.lastname: Fukunaga
authors.forename: Michinori
authors.initials: M
authors.lastname: Oikawa
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Sasai
authors.forename: Masayuki
authors.initials: M
authors.lastname: Kuzuya
mesh_terms: D006493:Heparin / Q000737:chemistry*; D006801:Humans; D010949:Plasma; D020959:Polyethylene / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D020959:Polyethylene; D006493:Heparin