[Study on the rapid detection method of Enterotoxigenic E. coli by polymerase chain reaction technology].
OBJECTIVE
To establish the rapid detection method of Enterotoxigenic E. coli (ETEC) by polymerase chain reaction (PCR) technology for characteristic primers.


METHODS
The primer of LT gene from Enterotoxigenic E. coli was designed and the condition of PCR was optimized to identify ETEC with PCR amplification for 16 samples.


RESULTS
all samples could not be amplified except ETEC with special primer.


CONCLUSIONS
The technology of ETEC determinated by PCR could be easy, rapid and accurate.
Li
Yufeng
Y
College of Bioengineering, Xihua University, Chengdu 610039, China.
Dai
Juan
J
Liu
Honglu
H
D017931:DNA Primers; D054307:Enterotoxigenic Escherichia coli / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*
D017931:DNA Primers