[Expression of TLR9 in human nasal epithelial cells].
OBJECTIVE
To analyze expression of TLR9 in human nasal epithelial cells by semi-quantitative reverse transcription-polymerase chain reaction, immunohistochemistry and flow cytometry.


METHODS
Human primary nasal epithelial cells (HNECs) were cultured, and then analyze the expression of TLR9 in primary cultured HNECs measured by semi-quantitative RT-PCR and immunohistochemistry and flow cytometry.


RESULTS
Primary cultured HNECs were observed by 400 x optical microscopes. Round or irregular cells stick to the bottom of cell culture plates. RT-PCR showed that mRNA expressions of TLR9 were found in primary nasal epithelial cells by 1% agarose gel electrophoresis. Interestingly, by analyzing with gray level of GAPDH, expression of TLR9 mRNA in HNECs was higher than positive control PBMCs.


CONCLUSIONS
TLR9 is expressed by HNECs. Expression of TLR9 mRNA in nasal epithelial cells is higher than in PBMCs.
Zhu
Xuewei
X
Department of Otolaryngology-Head and Neck Surgery, China-Japan Union Hospital, Jilin University, Changchun, 130033, China.
Zhu
Dongdong
D
Dong
Zhen
Z
Sun
Kewei
K
D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D009297:Nasal Mucosa / Q000166:cytology / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D051217:Toll-Like Receptor 9 / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; C495371:TLR9 protein, human; D051217:Toll-Like Receptor 9