Isolation and characterization of an anti-complementary protein-bound polysaccharide from the stem barks of Eucommia ulmoides.
EWDS-1, a homogeneous protein-bound polysaccharide, was isolated as an anti-complementary agent from the stem barks of Eucommia ulmoides. EWDS-1 was identified as a branched proteoglycan with average molecular weight about 2,000,000 Da, composed of Gal, Glc and Ara in the ratio of 2.1:1.0:0.9, along with trace of Rha, Xyl, Man, as well as 3.95% of protein. The linkages of the residues of EWDS-1 were deduced by methylation analysis and NMR technique. Bioassay showed that EWDS-1 inhibited complement activation on both the classic and alternative pathways with CH(50) and AP(50) values of 203+/-20 microg/ml and 45+/-8 microg/ml, respectively. Preliminary mechanism studies by using complement component depleted-sera indicated that EWDS-1 inhibits activation of complement system by interacting with C1q, C1r, C1s, C2, C3, C4, C5 and C9. The results suggested that EWDS-1 could be of promising benefits in treatment of the complement associated diseases.
Zhu
Hongwei
H
School of Pharmacy, Fudan University, Shanghai 200032, People's Republic of China.
Zhang
Yunyi
Y
Zhang
Jianwen
J
Chen
Daofeng
D
D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003167:Complement Activation / Q000187:drug effects; D051056:Complement Inactivating Agents; D004912:Erythrocytes / Q000187:drug effects; D031283:Eucommiaceae / Q000737:chemistry*; D006168:Guinea Pigs; D006801:Humans; D008745:Methylation; D051379:Mice; D024301:Plant Bark / Q000737:chemistry; D018547:Plant Stems / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology*; D011817:Rabbits; D012756:Sheep; D013918:Thrombin Time
D051056:Complement Inactivating Agents; D011134:Polysaccharides