[Proteomic study of paclitaxel on human cervical carcinoma HCE1].
OBJECTIVE
To explore the mechanism of paclitaxel on the protein expression of human cervical carcinoma cell line HCE1.


METHODS
The total proteins extracted from paclitaxel-treated HCE1 cells were analyzed by 2-dimensional gel electrophoresis (2-DE), and compared with those from untreated HCE1 cells. The differential proteins were identified by mass spectrometry. Western blot was used to determine the differential expression levels of the 2 proteins.


RESULTS
At 24 hour after paclitaxel (0.05 mumol/L) treatment, 2-DE images of paclitaxel-treated and paclitaxel-untreated cells were analyzed. Forty-two differential proteins were found. Twenty-one differential proteins among 42 proteins were analyzed by mass spectrometry, among which 15 proteins were identified, including peptidyl-prolylisomerases A (PPIase A),alpha-enolase,keratin 8,heat shock protein 90, eukaryotic translation initiation factor 1A, and so on.


CONCLUSIONS
Fifteen proteins in human cervical carcinoma cells paclitaxel-treated and paclitaxel-untreated are found by proteomic techniques. These proteins may be involved in the proliferation inhibition of human cervical carcinoma cells by paclitaxel.
Cao
Lan-Qin
LQ
Department of Gynecology and Obstetrics, Xiangya Hospital, Central South University, Changsha 410008, China. caolanqin@163.com
Lin
Qiu-Hua
QH
Li
Xin
X
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D014408:Biomarkers, Tumor / Q000032:analysis; D004268:DNA-Binding Proteins / Q000032:analysis; D015977:Eukaryotic Initiation Factor-1 / Q000032:analysis; D005260:Female; D020869:Gene Expression Profiling; D016678:Genome; D006801:Humans; D053533:Keratin-8 / Q000032:analysis; D017239:Paclitaxel / Q000494:pharmacology*; D010751:Phosphopyruvate Hydratase / Q000032:analysis; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D014407:Tumor Cells, Cultured; D025521:Tumor Suppressor Proteins / Q000032:analysis; D002583:Uterine Cervical Neoplasms / Q000378:metabolism* / Q000473:pathology
D000972:Antineoplastic Agents, Phytogenic; D014408:Biomarkers, Tumor; D004268:DNA-Binding Proteins; D015977:Eukaryotic Initiation Factor-1; D053533:Keratin-8; D020543:Proteome; D025521:Tumor Suppressor Proteins; C095157:ENO1 protein, human; D010751:Phosphopyruvate Hydratase; D017239:Paclitaxel