[Protective mechanism on the vascular pathological process in diabetes mellitus rats by Rheum officeinale].
OBJECTIVE
To explore the protective mechanism of officeihale on the vascular pathological process in diabetes mellitus (DM) rats.


METHODS
After the DM rat model was established, 24 DM rats were randomly divided into model group (12 DM rats) and Rheum officeinale group (12 DM rats). Rheum officeinale was orally given in 10 g kg(-1) per day, and the other two groups were given equal pure water. 8 weeks later, blood samples were collected to determine the level of nitric oxide (NO) and endothelin-1 (ET-1). Thoracic aortic rings was prepared to observe the inhibiting effect of Ach with different concentration on contraction caused by NE. Another part of aorta was made to observe the expression of ICAM-1 and VCAM-1 by method of SP immunohistochemistry staining,


RESULTS
Rheum officeinale group obviously decreased the level of ET-1 and increased the NO compared with model group (P <0.05). The expression of ICAM-1 and VCAM-1 could be obviously inhibited in Rheum officeinale group compared with model group. (P <0.05).


CONCLUSIONS
Rheum officeinale could decrease the level of ET-1 with increased the NO in diabetes rats, and inhibit the expression of ICAM-1 and VCAM-1, which may be mechanisms of protecting the endothelium of vessel in diabetes rats.
Tian
Feng-Sheng
FS
Endocrinology Department, CangZhou Affiliated hospital of integrated TCM-WM, Hebei Medical University, Cangzhou 061001, China. cztianfsh@sian.com
Li
Zhen-Bin
ZB
Wang
Yuan-Song
YS
Su
Xiu-Hai
XH
Li
Wen-Dong
WD
Wang
Xiao-Yun
XY
D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D001786:Blood Glucose / Q000378:metabolism; D001808:Blood Vessels / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D003920:Diabetes Mellitus / Q000473:pathology*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D019332:Endothelin-1 / Q000378:metabolism; D004730:Endothelium, Vascular / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D005786:Gene Expression Regulation / Q000187:drug effects; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D020011:Protective Agents / Q000494:pharmacology*; D051381:Rats; D012250:Rheum / Q000737:chemistry*
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; D020011:Protective Agents; D018799:Intercellular Adhesion Molecule-1; D009569:Nitric Oxide