Hydrolysis study of the bifunctional antitumour compound RAPTA-C, [Ru(eta6-p-cymene)Cl2(pta)].
The hydrolysis of [Ru(eta(6)-p-cymene)Cl(2)(PTA)] (PTA=1,3,5-triaza-7-phosphatricyclo-[3.3.1.1]decanephosphine; RAPTA-C) was studied using UV-visible (UV-vis) spectrophotometry and NMR spectroscopy. In analogy to in silico studies, [Ru(eta(6)-p-cymene)Cl(H(2)O)(PTA)](+) was found to be the most abundant hydrolysis product, although the dihydrolysed species [Ru(eta(6)-p-cymene)(OH)(H(2)O)(PTA)](+) and the dichloro compound are present. Rate constants for the different aquation and anation steps and the equilibrium constants were determined. Hydrolysis is suppressed at high chloride concentrations. These results have important implications on the mode of action of the RAPTA drug candidates.
Scolaro
Claudine
C
Institut des Sciences et Ingénierie Chimiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), 1015 Lausanne, Switzerland.
Hartinger
Christian G
CG
Allardyce
Claire S
CS
Keppler
Bernhard K
BK
Dyson
Paul J
PJ
D000970:Antineoplastic Agents / Q000737:chemistry*; D006868:Hydrolysis; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D017975:Ruthenium Compounds / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet
D000970:Antineoplastic Agents; D017975:Ruthenium Compounds