Biodosimetry using micronucleus assay in acute partial body therapeutic irradiation.
Biological dosimetry provides information on the absorbed dose and its distribution in the body for an early assessment of irradiation consequences in exposed individuals. In this study, an effort has been made to see the applicability of biological dosimetry using micronuclei assay for dose estimation in therapeutic irradiation of cancer patients in acute high dose partial body irradiation. Dose estimation in partial body irradiations was done on the basis of Dolphin's contaminated Poisson method, using the in vitro dose response calibration curve. The equivalent whole body dose and the dose to the irradiated part of the body were estimated to be 1.8+/-0.1 Gy and 6.4+/-0.3 Gy, respectively. The estimated percentage of irradiated blood and the fraction of cells exposed were 41.5+/-1.6% and 10.4+/-0.8%, respectively. The estimated fraction of irradiated cells was comparable with the actual volume of irradiation.
Senthamizhchelvan
Srinivasan
S
Department of Nuclear Medicine, All India Institute of Medical Sciences, Ansari nagar, New Delhi 110 029, India. senthamil_80@yahoo.com
Pant
G S
GS
Rath
G K
GK
Julka
P K
PK
Nair
O
O
D000328:Adult; D001681:Biological Assay / Q000379:methods*; D004307:Dose-Response Relationship, Radiation; D005260:Female; D006801:Humans; D008297:Male; D015162:Micronucleus Tests / Q000379:methods*; D008875:Middle Aged; D011874:Radiometry / Q000379:methods*; D011879:Radiotherapy Dosage; D020266:Radiotherapy, Conformal / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D055815:Young Adult