Minimizing a QTL region for intramuscular fat content by characterizing the porcine Phosphodiesterase 4B (PDE4B) gene.
Three isoforms of pig PDE4B were cloned and classified as two forms: PDE4B1 and PDE4B3, which contain UCR1 and UCR2; and PDE4B2, which contains only UCR2. The amino acid sequences of each isoform showed good conservation in human and rat. PDE4B2 is expressed in a wide range of tissues, but PDE4B1 and PDE4B3 are not. Using an informative SNP for the Iberian x Landrace intercross detected from intron 12, a linkage map was constructed. The location of PDE4B was estimated at 123.6 cM outside of the QTL-CI (124-128 cM) for IMF. However, the QTL-CI for IMF was reconfirmed with high significance, and its position was narrowed down to an interval of 4 cM (the region defined by markers PDE4B and SW1881). Using radiation hybrid mapping, LEPR, LEPROT, DNAJC6, AK3L1 and AK3L2 were selected as positional and/or functional candidates related to the QTL.
Kim
Jae-Hwan
JH
Division of Applied Life Science, Gyeongsang National University, Jinju, Korea.
Ovilo
Cristina
C
Park
Eung-Woo
EW
Fernndez
Almudena
A
Lee
Jun-Heon
JH
Jeon
Jin-Tae
JT
Lee
Jung-Gyu
JG
D000273:Adipose Tissue / Q000033:anatomy & histology*; D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002874:Chromosome Mapping; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4 / Q000737:chemistry / Q000235:genetics*; D017931:DNA Primers; D018076:DNA, Complementary; D008040:Genetic Linkage; D006801:Humans; D008969:Molecular Sequence Data; D009132:Muscles / Q000033:anatomy & histology*; D020641:Polymorphism, Single Nucleotide; D040641:Quantitative Trait Loci; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D013552:Swine
D017931:DNA Primers; D018076:DNA, Complementary; D012333:RNA, Messenger; D054703:Cyclic Nucleotide Phosphodiesterases, Type 4