Immunoassay using surface-enhanced Raman scattering based on aggregation of reporter-labeled immunogold nanoparticles.
A one-step homogenous sensitive immunoassay using surface-enhanced Raman scattering (SERS) has been developed. This strategy is based on the aggregation of Raman reporter-labeled immunogold nanoparticles induced by the immunoreaction with corresponding antigens. The aggregation of gold nanoparticles results in a SERS signal increase of the Raman reporter. Therefore, human IgG could be directly determined by measuring the Raman signal of the reporter. The process of aggregation was investigated by transmission electron microscopy (TEM) and UV-Vis absorption spectroscopy. The effects of the temperature, time, and size of gold nanoparticles on the sensitivity of the assay were examined. Using human IgG as a model protein, a wide linear dynamic range (0.1-15 microg mL(-1)) was reached with low detection limit (0.1 microg mL(-1)) under optimized assay conditions. The successful test suggests that the application of the proposed method holds promising potential for simple, fast detection of proteins in the fields of molecular biology and clinical diagnostics.
Chen
Ji-Wei
JW
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China.
Lei
Yong
Y
Liu
Xiang-Jiang
XJ
Jiang
Jian-Hui
JH
Shen
Guo-Li
GL
Yu
Ru-Qin
RQ
D000888:Antibodies, Anti-Idiotypic / Q000737:chemistry; D000937:Antigen-Antibody Reactions; D000941:Antigens / Q000737:chemistry; D006046:Gold / Q000737:chemistry*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G / Q000032:analysis*; D007150:Immunohistochemistry / Q000379:methods; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman / Q000379:methods*
D000888:Antibodies, Anti-Idiotypic; D000941:Antigens; D007074:Immunoglobulin G; C048844:anti-IgG; D006046:Gold