title: Determination of bromide in canine plasma using ion chromatography.
abstract: A new ion chromatographic procedure has been developed and validated for the determination of bromide in canine plasma. Following a simple dilution, samples were separated on a Metrosep A Supp 5 column. The mobile phase was an isocratic mixture of 2.2 mM Na(2)CO(3), 1.0 mM NaHCO(3), and 1% acetonitrile, with a flow-rate of 0.7 ml/min. The procedure produced a linear curve over the concentration range of 50-2500 microg/ml. The development of the assay permitted the determination of therapeutic levels after oral administration of potassium bromide to dogs being treated for epilepsy.
authors.affiliation: University of Tennessee, Department of Comparative Medicine, College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996, USA. scox6@utk.edu
authors.forename: S K
authors.initials: SK
authors.lastname: Cox
authors.forename: A M
authors.initials: AM
authors.lastname: Whiton
authors.forename: H L
authors.initials: HL
authors.lastname: Bowman
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000927:Anticonvulsants / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D001965:Bromides / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use; D002852:Chromatography, Ion Exchange / Q000379:methods*; D004283:Dog Diseases / Q000097:blood* / Q000188:drug therapy; D004285:Dogs; D004827:Epilepsy / Q000188:drug therapy / Q000662:veterinary*; D017680:Potassium Compounds / Q000008:administration & dosage / Q000097:blood* / Q000627:therapeutic use
chemical_list: D000927:Anticonvulsants; D001965:Bromides; D017680:Potassium Compounds; C039004:potassium bromide