title: Influence of alternating temperature preculture on cryopreservation results for potato shoot tips.
abstract: Cryopreservation is the most suitable long-term storage method for genetic resources of vegetatively maintained crops like potato. In the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) the DMSO droplet method is applied, and so far more than 1000 accessions are cryopreserved with an average regeneration rate of 58%. New experiments with four potato accessions using alternating temperatures (22/8 degrees C day/night temperature, 8 h photoperiod, 7 d) prior to cryopreservation showed improved regeneration. The influence of this preculture on the shoot tips was studied for two wild, frost resistant species Solanum acaule and S. demissum and for two cultivated, frost sensitive potatoes S. tuberosum 'Désirée' and 'King Edward'. Comparison of liquid and solid media after cryopreservation showed improved regeneration on solid media with higher regeneration percentages, less callus formation and better plantlet structure. In comparative analyses biochemical factors like soluble sugars, starch, and amino acid concentrations were measured. Shoot tips after constant and after alternating temperature preculture were analyzed. Total concentrations of soluble sugars (glucose, fructose, and sucrose) were higher for all accessions after the alternating temperature preculture, which could be the reason for improved cryopreservation results.
authors.affiliation: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466, Gatersleben, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Kaczmarczyk
authors.forename: Natalia
authors.initials: N
authors.lastname: Shvachko
authors.forename: Yulia
authors.initials: Y
authors.lastname: Lupysheva
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Hajirezaei
authors.forename: E R Joachim
authors.initials: ER
authors.lastname: Keller
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003470:Culture Media; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D005632:Fructose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D018520:Plant Shoots / Q000187:drug effects / Q000378:metabolism; D012038:Regeneration; D011198:Solanum tuberosum / Q000187:drug effects* / Q000378:metabolism; D013395:Sucrose / Q000378:metabolism; D013696:Temperature; D046509:Tissue Culture Techniques
chemical_list: D000596:Amino Acids; D003451:Cryoprotective Agents; D003470:Culture Media; D005632:Fructose; D013395:Sucrose; D005947:Glucose; D004121:Dimethyl Sulfoxide