Lipid-conjugated oligonucleotides via "click chemistry" efficiently inhibit hepatitis C virus translation.
Conjugation of a lipid moiety via "click chemistry" potentiates the cellular uptake of oligonucleotides and allows their intracellular delivery. These nontoxic lipid conjugates efficiently inhibit hepatitis C virus internal ribosome entry site (IRES)-mediated translation in human hepatic Huh7 cells. The biological activity of the lipid-conjugated oligonucleotides is not affected by the presence of serum.
Godeau
Guilhem
G
Inserm, U869, Bordeaux F-33076 France.
Staedel
Cathy
C
Barthélémy
Philippe
P
D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D016174:Hepacivirus / Q000187:drug effects* / Q000502:physiology*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D015394:Molecular Structure; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D014779:Virus Replication / Q000187:drug effects*
D008055:Lipids; D009841:Oligonucleotides