Fabrication of a novel glucose biosensor based on a highly electroactive polystyrene/polyaniline/Au nanocomposite.
A novel nanocomposite with a core-shell structure containing polystyrene (PS), polyaniline (PANI), and Au nanoparticles (NPs) was synthesized. The nanocomposite was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). Cyclic voltammetric experiments indicated that the nanocomposite had excellent redox ability in a wide range of pH values. The existence of Au NPs resulted in a higher electrical conductivity of the nanocomposite. As a model, glucose oxidase (GOD) was entrapped onto the nanocomposite-modified glassy carbon electrode (GCE) and applied to construct a sensor. The immobilized GOD showed a pair of well-defined redox peaks and high catalytic activity for the oxidation of glucose.
Liu
Yuge
Y
School of Chemistry and Chemical Engineering, Key Laboratory of Analytical Chemistry for Life Science (MOE), Nanjing University, Nanjing 210093, China.
Feng
Xiaomiao
X
Shen
Jianmin
J
Zhu
Jun-Jie
JJ
Hou
Wenhua
W
D000814:Aniline Compounds / Q000737:chemistry*; D001672:Biocompatible Materials / Q000138:chemical synthesis / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004553:Electric Conductivity; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D005947:Glucose / Q000378:metabolism*; D005949:Glucose Oxidase / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053768:Metal Nanoparticles / Q000737:chemistry*; D053761:Nanocomposites / Q000737:chemistry*; D011137:Polystyrenes / Q000737:chemistry*
D000814:Aniline Compounds; D001672:Biocompatible Materials; D011137:Polystyrenes; C416807:polyaniline; D006046:Gold; D005949:Glucose Oxidase; D005947:Glucose