Effects of different Helicobacter pylori culture filtrates on growth of gastric epithelial cells.
OBJECTIVE
To study the effects of different Helicobacter pylori (H pylori) culture filtrates on growth of gastric epithelial cells.


METHODS
Broth culture filtrates of H pylori were prepared. Gastric epithelial cells were treated with the filtrates, and cell growth was determined by growth curve and flow cytometry. DNA damage of gastric epithelial cells was measured by single-cell microgel electrophoresis.


RESULTS
Gastric epithelial cells proliferated actively when treated by CagA-gene-positive broth culture filtrates, and colony formation reached 40%. The number of cells in S phase increased compared to controls. Comet assay showed 41.2% comet cells in GES-1 cells treated with CagA-positive filtrates (P < 0.05).


CONCLUSIONS
CagA-positive filtrates enhance the changes in morphology and growth characteristics of human gastric epithelial tumor cells. DNA damage maybe one of the mechanisms involved in the growth changes.
Yan
Yan-Guo
YG
Department of General Surgery, The Affiliated Hospital of Wuhan University of Science & Technology, Wuhan 430064, Hubei Province, China. yan969400@yahoo.com.cn
Zhao
Gang
G
Ma
Jin-Ping
JP
Cai
Shi-Rong
SR
Zhan
Wen-Hua
WH
D000942:Antigens, Bacterial / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D015153:Blotting, Western; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D020552:Comet Assay; D017077:Culture Media, Conditioned / Q000378:metabolism; D004249:DNA Damage; D005434:Flow Cytometry; D005753:Gastric Mucosa / Q000378:metabolism*; D016480:Helicobacter pylori / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D019899:Paracrine Communication; D013997:Time Factors
D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D017077:Culture Media, Conditioned; C080866:cagA protein, Helicobacter pylori