Quaternary structure predictions and estimation of mutational effects on the free energy of dimerization of the OMPLA protein.
This study represents an extension to the outer membrane phospholipase A protein (OMPLA) of the docking-based protocols previously developed for quaternary structure predictions of transmembrane oligomeric proteins and for estimating mutational effects on the thermodynamics of protein-protein and protein-DNA association. Predictions of the likely architecture of OMPLA homo-dimers were carried out on 31 different forms of the monomer, 30 of which were variants of the unbound state. In all the test cases but the ones characterized by combined deletions of the 98-110 and 145-153 segments (L2 and L3, respectively), native-like complexes could be predicted, independent of the bound or unbound state of the structural model, of side chain conformation and presence or absence of amino acid deletions at the putative inter-monomer interface. The protocol for estimating mutational effects on the thermodynamics of protein-protein association proved effective as well. In fact, it was possible to estimate correctly the effects of five mutants on the free energy of dimerization of the sulfonylated form of OMPLA. The integrity of L2 and either one of the L1, L3 and L4 loops turned out to be more important than sulfonylation for the achievement of the native dimeric architecture. On the other hand, sulfonylation seems to be essential for a favorable dimerization energetics.
Dell'Orco
Daniele
D
Department of Chemistry, Dulbecco Telethon Institute (DTI), University of Modena e Reggio Emilia, Via Campi 183, 41100 Modena, Italy.
Casciari
Daniele
D
Fanelli
Francesca
F
D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics; D019281:Dimerization; D009154:Mutation; D054466:Phospholipases A1 / Q000737:chemistry* / Q000235:genetics; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D013441:Sulfinic Acids; D013816:Thermodynamics
D001425:Bacterial Outer Membrane Proteins; D013441:Sulfinic Acids; D054466:Phospholipases A1; C412876:outer membrane phospholipase A