[Inducing differentiation effect on cells of serum from mice treated with Yang He decoction].
OBJECTIVE
To observe inducing differention effect on cells of serum from mice treated with Yang He decoction (SMY).


METHODS
The viability of B16 cells was tested by lactate dehydrogenase (LDH) release assay and sulforhordamine B (SRB) assay. The differentiation of 3T3-L1 preadipocyte, HL-60, K562 and B16 cells were assessed by microscopy and Oil red O staining, NBT reduction and phagocytosis, haemoglobin (Hb) content and melanin content, respectively.


RESULTS
SMY could obviously inhibit growth of B16 cells without cytotoxicity, and promote Oil red O staining in 3T3-L1 preadipocyte, enhance the NBT reduction ability and the phagocytosis of horse radish peroxidase in HL-60 cells, increase Hb content in K562 cells, and decrease melanin content in B16 cells.


CONCLUSIONS
SMY is able to induce functional differentiation and maturation of 3T3-L1 preadipocyte and tumor cells, which implies the major mechanism of Yang He decoction in antitumor.
Du
Gang-jun
GJ
Department of Pharmacology, Pharmacy College of Henan University, Kaifeng 475001, China. kfdgj@sohu.com
Lin
Hai-hong
HH
Xie
Song-qiang
SQ
D041721:3T3-L1 Cells; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D002454:Cell Differentiation / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D003470:Culture Media / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D020014:K562 Cells; D008297:Male; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry*; D044967:Serum
D000972:Antineoplastic Agents, Phytogenic; D003470:Culture Media; D004338:Drug Combinations; D004365:Drugs, Chinese Herbal; C533613:yanghe