Analysis of lolB gene sequence and its use in the development of a PCR assay for the detection of Vibrio cholerae.
A PCR assay has been developed based on a lolB (hemM) gene, which was found to be highly conserved among the Vibrio cholerae species but non-conserved among the other enteric bacteria. The lolB PCR detected all O1, O139 and non-O1/non-O139 serogroup and biotypes of V. cholerae. The analytical specificity of this assay was 100% while the analytical sensitivity was 10 pg/microL and 10(3) CFU/mL at DNA and bacterial level respectively. The diagnostic sensitivity and specificity was 98.5% and 100% respectively.
Lalitha
P
P
School of Health Science, Universiti Sains Malaysia, Malaysia. lalitha@kb.usm.my
Siti Suraiya
M N
MN
Lim
K L
KL
Lee
S Y
SY
Nur Haslindawaty
A R
AR
Chan
Y Y
YY
Ismail
Asma
A
Zainuddin
Zainul F
ZF
Ravichandran
M
M
D001426:Bacterial Proteins / Q000235:genetics*; D003304:Coproporphyrinogen Oxidase / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D006090:Gram-Negative Bacteria / Q000235:genetics / Q000302:isolation & purification; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D014735:Vibrio Infections / Q000382:microbiology*; D014734:Vibrio cholerae / Q000235:genetics* / Q000302:isolation & purification*
D001426:Bacterial Proteins; D003304:Coproporphyrinogen Oxidase; D004269:DNA, Bacterial; C109194:HemN protein, Bacteria