Echinococcus granulosus: induction of T-independent antibody response against protoscolex glycoconjugates in early experimental infection.
In this work CD4-knockout mice were used as a model to analyse the role of CD4+ T cells in the antibody response against Echinococcus granulosus immunization or experimental infection. Results obtained with mice immunized with protoscolex antigens indicated that these contain T-independent antigens. After infection, CD4-knockout mice and C57Bl/6 mice showed similar titres of specific antibodies indicating that T-independent antibody production was quantitatively important in early infection. We have also identified an antigenic fraction from protoscoleces (E4+) which induces CD4 T cell independent antibody response in early stages of infection. In conclusion, the results presented here directly support the existence of T-independent immunogens in E. granulosus protoscoleces and suggest that T-independent antibody response may be quantitatively important in early infection.
Baz
Adriana
A
Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
Carol
Hernán
H
Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
Fernández
Verónica
V
Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
Mourglia-Ettlin
Gustavo
G
Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
Nieto
Alberto
A
Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
Örn
Anders
A
Immunology Laboratory, Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
Dematteis
Sylvia
S
Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D015704:CD4 Antigens / Q000235:genetics; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004443:Echinococcosis / Q000276:immunology*; D048209:Echinococcus granulosus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006001:Glycoconjugates / Q000276:immunology; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood / Q000145:classification; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015704:CD4 Antigens; D000939:Epitopes; D006001:Glycoconjugates; D007074:Immunoglobulin G