Which are you watching, an individual reactive oxygen species or total oxidative stress?
The fluorometric measurement of an individual reactive oxygen species (ROS) provides useful biological and biochemical information on ROS as important mediators for the pathological conditions of various diseases and yet requires a highly specific probe toward the target ROS. To design such a specific ROS probe, this report proposes a new strategy based on protection-deprotection chemistry between fluoresceins and their derivatives protected with benzenesulfonyl groups. The strategy has allowed developing new fluorescent probes toward extra- and intracellular hydrogen peroxide or superoxide. Herein, I outline the strategy used for the design of these ROS probes and their probe performance with high selectivity toward hydrogen peroxide and superoxide, which could not be achieved until a simple deprotection, namely, a nonredox reaction, was used as a fluorescing process.
Maeda
Hatsuo
H
Hyogo University of Health Sciences, Department of Pharmacy, Division of Bioanalytical Chemistry, Kobe, Japan. hmaeda@huhs.ac.jp
D000818:Animals; D004305:Dose-Response Relationship, Drug; D005456:Fluorescent Dyes / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D017665:Hydroxyl Radical; D008856:Microscopy, Fluorescence / Q000295:instrumentation* / Q000379:methods; D008858:Microscopy, Phase-Contrast; D008956:Models, Chemical; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry; D017382:Reactive Oxygen Species; D013481:Superoxides / Q000378:metabolism; D013997:Time Factors
D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D013481:Superoxides; D017665:Hydroxyl Radical; D006861:Hydrogen Peroxide; D010100:Oxygen