title: Highly sensitive and fast responsive fluorescence turn-on chemodosimeter for Cu2+ and its application in live cell imaging.
abstract: A rhodamine B derivative 4 containing a highly electron-rich S atom has been synthesized as a fluorescence turn-on chemodosimeter for Cu(2+). Following Cu(2+)-promoted ring-opening, redox and hydrolysis reactions, comparable amplifications of absorption and fluorescence signals were observed upon addition of Cu(2+); this suggests that chemodosimeter 4 effectively avoided the fluorescence quenching caused by the paramagnetic nature of Cu(2+). Importantly, 4 can selectively recognize Cu(2+) in aqueous media in the presence of other trace metal ions in organisms (such as Fe(3+), Fe(2+), Cu(+), Zn(2+), Cr(3+), Mn(2+), Co(2+), and Ni(2+)), abundant cellular cations (such as Na(+), K(+), Mg(2+), and Ca(2+)), and the prevalent toxic metal ions in the environment (such as Pb(2+) and Cd(2+)) with high sensitivity (detection limit < or =10 ppb) and a rapid response time (< or =1 min). Moreover, by virtue of the chemodosimeter as fluorescent probe for Cu(2+), confocal and two-photon microscopy experiments revealed a significant increase of intracellular Cu(2+) concentration and the subcellular distribution of Cu(2+), which was internalized into the living HeLa cells upon incubation in growth medium supplemented with 50 muM CuCl(2) for 20 h.
authors.affiliation: Department of Chemistry & Laboratory of Advanced Materials, Fudan University, 220 Handan Road, Shanghai, PR China.
authors.forename: Mengxiao
authors.initials: M
authors.lastname: Yu
authors.forename: Mei
authors.initials: M
authors.lastname: Shi
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Chen
authors.forename: Fuyou
authors.initials: F
authors.lastname: Li
authors.forename: Xinxin
authors.initials: X
authors.lastname: Li
authors.forename: Yanhong
authors.initials: Y
authors.lastname: Gao
authors.forename: Jia
authors.initials: J
authors.lastname: Xu
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Zhou
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.forename: Chunhui
authors.initials: C
authors.lastname: Huang
mesh_terms: D002413:Cations, Divalent; D045744:Cell Line, Tumor; D002470:Cell Survival; D003300:Copper / Q000737:chemistry* / Q000378:metabolism*; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D008958:Models, Molecular; D008969:Molecular Sequence Data; D012680:Sensitivity and Specificity; D013997:Time Factors
chemical_list: D002413:Cations, Divalent; D005456:Fluorescent Dyes; D003300:Copper