Mitosis-dependent protein expression in neuroblastoma cell line N1E-115.
Systematic work on differential protein expression in mitosis is limited, and we therefore used neuroblastoma cells (N1E-115) incubated with either colcemid or nocodazole to arrest mitosis. Proteins were identified by MALDI-TOF/TOF and nano-LC-ESI-MS/MS with subsequent quantification of spot volumes with specific software. Immunoblotting was used for verification of selected proteins. Levels of 10 individual proteins were increased and levels of 6 proteins were decreased concordantly by both treatments. These proteins were constituents of heat shock and chaperone, cytoskeleton, proteasomal, heterochromatin, and DNA replication signaling as well as housekeeping and metabolic systems. Identification of mitosis-dependent proteins is of importance for the interpretation of previous work and for designing future experiments.
Azizi
Amedeo A
AA
Department of Pediatrics and Adolescent Medicine, Medical University of Vienna, Wahringer Gurtel 18-20, Vienna, Austria.
Kang
Sung-Ung
SU
Freilinger
Angelika
A
Gruber-Olipitz
Mariella
M
Chen
Wei-Qiang
WQ
Yang
Jae-Won
JW
Hengstschläger
Markus
M
Slavc
Irene
I
Lubec
Gert
G
D000818:Animals; D045744:Cell Line, Tumor; D002853:Chromatography, Liquid; D002868:Chromosomal Proteins, Non-Histone / Q000096:biosynthesis; D003598:Cytoskeletal Proteins / Q000096:biosynthesis; D004261:DNA Replication / Q000502:physiology; D004268:DNA-Binding Proteins; D003703:Demecolcine / Q000494:pharmacology; D006360:Heat-Shock Proteins / Q000096:biosynthesis; D051379:Mice; D008938:Mitosis / Q000187:drug effects / Q000502:physiology*; D018832:Molecular Chaperones / Q000096:biosynthesis; D009447:Neuroblastoma; D015739:Nocodazole / Q000494:pharmacology; D009687:Nuclear Proteins / Q000096:biosynthesis; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D011506:Proteins / Q000378:metabolism*; D016601:RNA-Binding Proteins; D015398:Signal Transduction; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D053719:Tandem Mass Spectrometry; D050257:Tubulin Modulators / Q000494:pharmacology
D002868:Chromosomal Proteins, Non-Histone; D003598:Cytoskeletal Proteins; D004268:DNA-Binding Proteins; D006360:Heat-Shock Proteins; D018832:Molecular Chaperones; D009687:Nuclear Proteins; C095938:Pa2g4 protein, mouse; D011506:Proteins; D016601:RNA-Binding Proteins; D050257:Tubulin Modulators; D046988:Proteasome Endopeptidase Complex; D015739:Nocodazole; D003703:Demecolcine