title: The Campylobacter jejuni/coli cjaA (cj0982c) gene encodes an N-glycosylated lipoprotein localized in the inner membrane.
abstract: The Campylobacter coli 72Dz/92 cjaA gene (orthologue of cj0982c of C. jejuni NCTC 11168) product is a highly immunogenic, amino acid-binding protein. CjaA was palmitic acid-modified when processed in E. coli. In addition, site-directed mutagenesis of the Cys residue of the LAAC motif of its signal sequence confirmed that CjaA is a lipoprotein when processed in Campylobacter. Localization of the protein appeared to be host dependent. In Campylobacter, CjaA was recovered mainly as an inner-membrane protein, whereas in E. coli most of the protein was present in the periplasmic space. Interestingly, antiserum raised against Campylobacter glycine-extracted material also recognized CjaA produced by Campylobacter and Escherichia coli, indicating that at least part of the protein may be surface exposed. Site-directed mutagenesis of the Asn residues of two putative N-linked glycosylation sites (NIS and NFT) showed that CjaA is glycosylated and that only the first N-X-S/T sequeon serves as a glycan acceptor.
authors.affiliation: Department of Bacterial Genetics, Institute of Microbiology, University of Warsaw, Warsaw, Poland. agawysz@biol.uw.edu.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Wyszyńska
authors.forename: Joanna
authors.initials: J
authors.lastname: Zycka
authors.forename: Renata
authors.initials: R
authors.lastname: Godlewska
authors.forename: Elzbieta K
authors.initials: EK
authors.lastname: Jagusztyn-Krynicka
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000235:genetics / Q000378:metabolism*; D026921:Amino Acid Transport Systems, Neutral / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D017000:Campylobacter coli / Q000235:genetics / Q000378:metabolism*; D016123:Campylobacter jejuni / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D021382:Protein Sorting Signals
chemical_list: D018528:ATP-Binding Cassette Transporters; D026921:Amino Acid Transport Systems, Neutral; D001426:Bacterial Proteins; C503090:CjaA protein, Campylobacter jejuni; D008074:Lipoproteins; D008565:Membrane Proteins; D021382:Protein Sorting Signals