Acute cellular rejection monitoring after intestinal transplant: utility of serologic markers and zoom videoendoscopy as support of conventional biopsy and clinical findings.
Acute cellular rejection (ACR) episodes in intestinal transplant recipients are diagnosed by histologic and clinical findings. We have applied zoom video endoscopy and the use of serologic markers granzyme B (GrB) and perforin (PrF) to monitor rejection together with conventional tools. Seven hundred eighty-two blood samples (obtained at the time of the biopsy) collected from 34 recipients for GrB/PrF upregulation were positive among 64.9% of ACRs during a 3-year follow-up. Considering only the first year results posttransplantation, it reached 73.1% of rejection events. Zoom videoendoscopy was used by our group in 29 recipients of isolated intestine (n = 24) or multivisceral transplantations (n = 5) to enable observation of villi and crypt areas. From more than 270 procedures, 84% of the zoom findings agreed with the histologic results, namely, a specificity of 95%. In fact, during ongoing ACR, villi were altered in 80% of cases. Both procedures were helpful to support conventional histologic findings and clinical symptoms of ACR in intestinal transplant recipients.
Lauro
A
A
Liver and Multiorgan Transplant Unit, St.Orsola-Malpighi Hospital, University of Bologna, Bologna, Italy. augustola@yahoo.com
Altimari
A
A
Di Simone
M
M
Dazzi
A
A
Cescon
M
M
Zanfi
C
C
Miklosova
Z
Z
Corti
B
B
Gruppioni
E
E
D'Errico
A
A
Cautero
N
N
Giustozzi
G
G
Ansaloni
L
L
Ramacciato
G
G
Gruttadauria
S
S
Gruttadauria
G
G
Pinna
A D
AD
D000208:Acute Disease; D001706:Biopsy; D004724:Endoscopy; D006084:Graft Rejection / Q000276:immunology / Q000473:pathology*; D053804:Granzymes / Q000097:blood; D006801:Humans; D007111:Immunity, Cellular; D007422:Intestines / Q000637:transplantation*; D018715:Microscopy, Video; D015166:Monitoring, Immunologic / Q000379:methods; D008991:Monitoring, Physiologic; D054353:Perforin / Q000097:blood
D054353:Perforin; D053804:Granzymes