title: [Experimental studies of long-term effect of a detergent disinfecting for surface on a strain of Escherichia coli].
abstract: We offer three complementary, original methods and reproducibles to study the antibacterial and long-term effect of a detergent disinfecting for surfaces commercialized lately in France. Long-term activity noticed-effect is compared with that of other products. We first study the curves of growth of a strain of Escherichia coli put in presence with the surface of the wells of a microplate beforehand and for several days (from D-10 to D0), coated with disinfecting detergents. Another method consisted on surfaces firstly treated from D-10 to D0 by the one or the other product to be tested, which are artificially contaminated in a standardized manner by a velvet footprint with a suspension of E. coli. The surviving microbes are counted after transfer on a Rodac plate. Finally, doorhandles are cleaned and disinfected with the product. The natural bacterial recolonization doorhandles is studied by daily Rodac plate within a week. These studies allow to prove that Bacoban introduces a bactericidal activity on E. coli with an long-term effect for at least 10 days. The most competitive products have a bacteriostatic effect during nine to 10 days, but bactericidal effect only during two days. This bactericidal long-term effect may be particularly interesting in hospital hygiene for the biocleaning of the most manipulated surfaces and should restrict the role of bacterial reservoir of certain surfaces participating in care or near of the patients.
authors.affiliation: Pôle de spécialités médicales-ophtalmologie-hygiène, les hôpitaux universitaires de Strasbourg, 67091 Strasbourg cedex, France.
authors.forename: A
authors.initials: A
authors.lastname: Schmitt
authors.forename: N
authors.initials: N
authors.lastname: Glasser
authors.forename: D
authors.initials: D
authors.lastname: Steinbach
authors.forename: O
authors.initials: O
authors.lastname: Meunier
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003902:Detergents / Q000494:pharmacology*; D004202:Disinfectants / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects* / Q000254:growth & development; D006761:Hospitals / Q000592:standards; D006801:Humans; D012680:Sensitivity and Specificity; D013499:Surface Properties; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D003902:Detergents; D004202:Disinfectants