Effects of subinhibitory concentrations of antibiotics on SOS and DNA repair gene expression in Staphylococcus aureus.
Reporter clones of Staphylococcus aureus with different SOS response- and DNA repair-associated promoter-lux gene fusion constructs were constructed to study the effects of sub-MICs of antibiotics on the transcription of the SOS and methyl mismatch repair (MMR) genes. Fluoroquinolones (FQs) upmodulated both the SOS and the MMR genes. The patterns of antibiotic-induced transcriptional modulation were altered in FQ-resistant mutants.
Mesak
Lili Rosana
LR
Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada. mesak@interchange.ubc.ca
Miao
Vivian
V
Davies
Julian
J
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D053843:DNA Mismatch Repair; D004260:DNA Repair / Q000187:drug effects*; D024881:Drug Resistance, Bacterial; D024841:Fluoroquinolones / Q000494:pharmacology*; D015964:Gene Expression Regulation, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests; D011401:Promoter Regions, Genetic / Q000235:genetics / Q000502:physiology; D011993:Recombinant Fusion Proteins / Q000235:genetics; D013014:SOS Response, Genetics / Q000187:drug effects*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
D000900:Anti-Bacterial Agents; D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D024841:Fluoroquinolones; C504848:Lux Arrhythmo protein, Arabidopsis; D011993:Recombinant Fusion Proteins; D014157:Transcription Factors