Semicontinuous cultivation of Digitalis lanata cells: production of beta-methyldigoxin in a 300-L airlift bioreactor.
Selected Digitalis lanata cell lines cultivated in 1-L shake flasks or 20-L airlift bioreactors converted beta-methyldigitoxin into beta-methyldigoxin with almost no side reactions. This biotransformation process was optimized with regard to substrate supply and culture medium composition, and was then scaled up to a volume of 210 L using a 300-L airlift bioreactor. A semicontinuous process was developed in which 513.3 g beta-methyldigoxin were produced after 89 days of cultivation.
Reinhard
E
E
Pharmaceutical Institute, University of Tuebingen, D-7400 Tuebingen, Federal Republic of Germany.
Kreis
W
W
Barthlen
U
U
Helmbold
U
U