title: Purification and characterization of a natural lectin from the plasma of the shrimp Fenneropenaeus chinensis.
abstract: A natural lectin from the plasma of the shrimp Fenneropenaeus chinensis was purified by singlestep affinity chromatography using fetuin-coupled agarose. The purified plasma lectin showed a strong affinity for human A/B/O erythrocytes (RBC), mouse RBC and chicken RBC. The hemagglutinating (HA) activity of the lectin was dependent on Ca(2+) and reversibly sensitive to EDTA. This lectin was named FC-L and its inactive form had a molecular mass estimate of 168 kDa. Fifteen N-terminal amino acid sequences of this protein were determined. We performed HA-inhibition assays with several carbohydrates and glycoproteins. FC-L showed a distinct and unique specificity to N-acetylated sugars, particularly sialic acid and sialoproteins. The FC-L also has binding activity to some Gram-negative bacteria which caused disease in shrimp and fish. The activity of FC-L was inhibited at temperatures greater than 75 degrees C and at a pH less than 7 or greater than 11. These results suggest that FC-L may play a role as pattern recognition proteins in the reorganization and clearance of invaders in shrimp F. chinensis.
authors.affiliation: Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Sun
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Baojie
authors.initials: B
authors.lastname: Wang
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Guo
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
authors.forename: Keyong
authors.initials: K
authors.lastname: Jiang
authors.forename: Ran
authors.initials: R
authors.lastname: Tao
authors.forename: Guofan
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001419:Bacteria / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D050260:Carbohydrate Metabolism; D004912:Erythrocytes / Q000378:metabolism; D006385:Hemagglutination Inhibition Tests; D006386:Hemagglutination Tests; D006458:Hemolymph / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D037102:Lectins / Q000302:isolation & purification* / Q000378:metabolism*; D033561:Penaeidae / Q000378:metabolism*; D013696:Temperature
chemical_list: D037102:Lectins; D002118:Calcium