Screening genes that change expression during compression wood formation in Chamaecyparis obtusa.
We screened cDNA fragments that change their expression during compression wood formation by fluorescent differential display (FDD) in five adult trees (Chamaecyparis obtusa (Siebold & Zucc.) Endl.) growing naturally at an angle to the vertical, and in two saplings, one vertical, the other inclined. We conducted anatomical observations and measurements of the released strain of growth stress on the five adult trees to confirm that they formed compression wood on the lower side of the inclined trunks. Based on sequencing results from selected cDNA fragments, we conducted homology searches of the GenBank database and designed specific primers for the 67 screened fragments. Using these primers and different saplings from those used for the FDD screening, we tested the expression levels of each fragment in normal, compression and opposite wood regions of saplings by semiquantitative reverse-transcription polymerase chain reaction. Twenty-four fragments showed reproducible expression patterns, indicating that these fragments changed their expression during compression wood formation. Some fragments showed differential expression between the apical and basal regions of the lower side of the inclined stem in the region of compression wood formation. Anatomical observations indicated more intense compression wood formation in the basal region than in the apical region of the stem, demonstrating a relationship between compression wood development and gene expression.
Yamashita
Saori
S
Laboratory of Bio-material Physics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464-8601, Japan.
Yoshida
Masato
M
Yamamoto
Hiroyuki
H
Okuyama
Takashi
T
D001696:Biomechanical Phenomena; D029780:Chamaecyparis / Q000033:anatomy & histology / Q000235:genetics* / Q000254:growth & development; D018076:DNA, Complementary / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid; D014934:Wood / Q000033:anatomy & histology / Q000254:growth & development*
D018076:DNA, Complementary