A segregated model of recombinant multicopy plasmid propagation.
A segregated model of multicopy plasmid propagation has been formulated which incorporates plasmid replication and partition functions, as well as the effect of plasmid presence on host growth rate. Growth of plasmid-free cells in selective medium is explicitly analyzed. The model parameters can be determined from experimentally measurable quantities. Propagation of a recombinant multicopy plasmid in the yeast Saccharomyces cerevisiae is analyzed using this model.
Wittrup
K D
KD
Department of Chemical Engineering, California Institute of Technology, Pasadena, California 91125.
Bailey
J E
JE