Immunohistochemical localization of telomerase in myomas and in uterine myometrium.
Telomerase activity could be a potential marker for the neoplastic process, because it is absent in normal cells and present in tumor cells. Immunohistochemical studies were conducted using samples obtained from 32 uterine myomas, each sample having a size of 3-4 cm and obtained from women between 35 and 45 years of age. These studies also concentrated on fragments of macroscopically unaltered myometrium, collected 3-4 cm from a uterine tumor. Immunohistochemistry was performed using antibody to the catalytic unit of telomerase (hTERT; clone 44F12, NCL-L-hTERT, Novocastra Laboratories, UK). This study aimed at detecting a possible presence of potentially neoplastic cells in the margins of healthy tissue, which was removed together with the primary tumor. The results were classified according to the number of telomerase-positive cells. Tumors of the first group had up to 50% telomerase-positive cells, while their content in the second group exceeded 50%. Our study demonstrated an almost two-fold increase in the number of telomerase-positive tumor cells compared with myometrial cells 3-4 cm from the tumor. Hopefully, investigating the presence of telomerase in both uterine myometrium and myoma could facilitate the diagnosis of the neoplastic process.
Madej
Paweł
P
Department of Gynaecological Endocrinology, Medical University of Silesia, Poland. pmadej@slam.katowice.pl
Plewka
Andrzej
A
Madej
Janusz A
JA
Dzimira
Stanisław
S
Nowak
Marcin
M
Plewka
Danuta
D
Nowaczyk
Grazyna
G
D000328:Adult; D014408:Biomarkers, Tumor / Q000032:analysis*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007889:Leiomyoma / Q000201:enzymology*; D008875:Middle Aged; D009215:Myometrium / Q000201:enzymology*; D019098:Telomerase / Q000096:biosynthesis*; D014594:Uterine Neoplasms / Q000201:enzymology*
D014408:Biomarkers, Tumor; D019098:Telomerase