Thyrotropin-releasing hormone (TRH) reverses hyperglycemia in rat.
Hyperglycemia in thyrotropin-releasing hormone (TRH) null mice indicates that TRH is involved in the regulation of glucose homeostasis. Further, TRH levels in the pancreas peak during the stages of late embryonic and early neonatal beta cell development. These observations are consistent in linking TRH to islet cell proliferation and differentiation. In this study, we examined the effect of TRH administration in damaged pancreatic rat (streptozotocin, STZ) to determine whether TRH could improve damaged pancreatic beta cells function. We hypothesize that TRH is able to reverse STZ-induced hyperglycemia by increasing pancreatic islet insulin content, preventing apoptosis, and potentially induce islet regeneration. It was found that following intra-peritoneal (ip) injection, TRH (10 microg/kg body weight (bwt)) reverses STZ (65 mg/kg bwt)-induced hyperglycemia (TRH given 3 days after STZ injection). Increased circulating insulin levels and insulin content in extracted pancreas suggests that TRH reversed STZ-induced hyperglycemia through improving pancreatic islet beta cell function. Further studies show a significantly lower level of apoptosis in islets treated with TRH as well as the presence of proliferation marker nestin and Brdu, suggesting that the TRH has the potential to prevent apoptosis and stimulate islet proliferation.
Luo
LuGuang
L
The Center of Stem Cell Biology, Department of Research, Roger Williams Hospital, 825 Chalkstone Avenue, Providence, RI 02908, USA. Lluo@rwmc.org
Luo
John Z Q
JZ
Jackson
Ivor M D
IM
D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation; D006943:Hyperglycemia / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D007328:Insulin / Q000097:blood / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D051379:Mice; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration / Q000187:drug effects; D013311:Streptozocin / Q000633:toxicity; D013973:Thyrotropin-Releasing Hormone / Q000008:administration & dosage*
D007328:Insulin; D013311:Streptozocin; D013973:Thyrotropin-Releasing Hormone