Molecular cloning and functional characterization of a novel isoform of chicken myeloid differentiation factor 88 (MyD88).
Myeloid differentiation factor 88 (MyD88) is an adaptor protein involved in the interleukin-1 receptor- and Toll-like receptor-induced activation of nuclear factor-kappaB (NF-kappaB). A novel isoform of chicken MyD88, designated chicken MyD88-2, has been cloned and functionally characterized. Its open reading frame is of length 900bp, and it encodes a predicted 299 residue protein, similar in length to its mammalian orthologues, but, respectively, 77 and 69 amino acids shorter than the previously described chicken MyD88-1 and -3. The amino acid sequence of chicken MyD88-2 displays 96.9%, 96.9%, 70.4% and 70.2% identity with, respectively, chicken MyD88-1, -3, human and mouse MyD88. Chicken MyD88-2 expression was detected in a range of tissues tested, but no expression of either chicken MyD88-1 or -3 was observed. The over-expression of chicken MyD88-2 significantly induced the activation of NF-kappaB in vitro, suggesting that chicken MyD88-2 plays an important role in the innate immune responses of chicken.
Qiu
Yafeng
Y
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science, No. 3 Lane 345, Shilong Road, Shanghai 200232, PR China.
Shen
Yang
Y
Li
Xiangdong
X
Ding
Chan
C
Ma
Zhiyong
Z
D000595:Amino Acid Sequence; D000818:Animals; D030161:Avian Proteins / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D001483:Base Sequence; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D002645:Chickens / Q000235:genetics* / Q000378:metabolism; D003001:Cloning, Molecular; D006801:Humans; D008969:Molecular Sequence Data; D053594:Myeloid Differentiation Factor 88 / Q000737:chemistry* / Q000235:genetics* / Q000502:physiology; D016328:NF-kappa B / Q000378:metabolism; D020033:Protein Isoforms / Q000737:chemistry / Q000235:genetics / Q000502:physiology
D030161:Avian Proteins; D053594:Myeloid Differentiation Factor 88; D016328:NF-kappa B; D020033:Protein Isoforms