In vitro study of DNA interaction with clodinafop-propargyl herbicide.
The interaction of native calf thymus DNA with clodinafop-propargyl (CP), in 10 mM HEPES aqueous solutions at neutral pH 7.2, has been investigated by spectrophotometric, circular dichroism (CD), spectrofluorometric, melting temperature (Tm), and viscosimetric techniques. It was found that CP molecules could intercalate between base pairs of DNA as evidenced by hyperchromism in UV absorption band of DNA, an increase in melting temperature, a sharp increase in specific viscosity of DNA, induced CD spectral changes, and increase in the fluorescence of methylene blue (MB)-DNA solutions in the presence of increasing amounts of CP, which indicates that it is able to release the intercalated MB completely. All results suggest that the CP interacts with calf thymus DNA by an intercalative mode of binding.
Kashanian
Soheila
S
Department of Chemistry & Sensor and Biosensor Research Center, Faculty of Science, Razi University, Kermanshah, I.R. Iran. kashanian_s@yahoo.com
Askari
Soyebeh
S
Ahmadi
Farhad
F
Omidfar
Kobra
K
Ghobadi
Sirous
S
Tarighat
Fereshteh Abasi
FA
D000103:Acetyl-CoA Carboxylase / Q000037:antagonists & inhibitors; D000818:Animals; D002417:Cattle; D002942:Circular Dichroism; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D005453:Fluorescence; D006540:Herbicides / Q000737:chemistry / Q000378:metabolism*; D007364:Intercalating Agents / Q000737:chemistry / Q000378:metabolism*; D009691:Nucleic Acid Denaturation; D011422:Propionates / Q000737:chemistry / Q000378:metabolism*; D011725:Pyridines / Q000737:chemistry / Q000378:metabolism*; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013816:Thermodynamics; D014783:Viscosity
D006540:Herbicides; D007364:Intercalating Agents; D011422:Propionates; D011725:Pyridines; D004247:DNA; C427203:calf thymus DNA; D000103:Acetyl-CoA Carboxylase; C400760:clodinafop