title: Prolonged stochastic single ion channel recordings in S-layer protein stabilized lipid bilayer membranes.
abstract: S-layer proteins are commonly found in bacteria and archaea as two-dimensional monomolecular crystalline arrays as the outermost cell membrane component. These proteins have the unique property that following disruption by chemical agents, monomers of the protein can re-assemble to their original lattice structure. This unique property makes S-layers interesting for utilization in bio-nanotechnological applications. Here, we show that the addition of S-layer proteins to bilayer lipid membranes increases the lifetime and the stability of the bilayer. M2delta ion channels were functionally incorporated into these S-layer stabilized membranes and we were able to record their activity for up to 20 h. Transmission electron microscopy (TEM) was used to visualize the 2D crystalline pattern of the S-layer and the M2delta ion channel characteristics in bilayer lipid membrane's were compared in the presence and absence of S-layers.
authors.affiliation: Department of Chemistry, Physical Chemistry, University of Florida, 318 Leigh Hall, FL 32611, Gainesville, USA.
authors.forename: Henk M
authors.initials: HM
authors.lastname: Keizer
authors.forename: Martin
authors.initials: M
authors.lastname: Andersson
authors.forename: Chris
authors.initials: C
authors.lastname: Chase
authors.forename: William P
authors.initials: WP
authors.lastname: Laratta
authors.forename: Josh B
authors.initials: JB
authors.lastname: Proemsey
authors.forename: Joel
authors.initials: J
authors.lastname: Tabb
authors.forename: Joanna R
authors.initials: JR
authors.lastname: Long
authors.forename: Randolph S
authors.initials: RS
authors.lastname: Duran
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry*; D003460:Crystallization; D007473:Ion Channels / Q000502:physiology*; D008051:Lipid Bilayers; D008562:Membrane Glycoproteins / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D018408:Patch-Clamp Techniques; D013269:Stochastic Processes
chemical_list: D001426:Bacterial Proteins; D007473:Ion Channels; D008051:Lipid Bilayers; D008562:Membrane Glycoproteins; C055764:S-layer proteins