Molecular characterization of Burkholderia strains isolated from rice cultivars (Oryza sativa L.) for species identification and phylogenetic grouping.
The genus Burkholderia consists of extremely versatile bacteria that occupy diverse niches and are commonly encountered in the rhizosphere of crop plants. In this study, we characterized three plant growth promoting strains assigned as Burkholderia sp. using biochemical and molecular characterization. The Burkholderia spp. strains CBMB40, CBPB-HIM, and CBPB-HOD were characterized using biochemical tests, BIOLOG carbon substrate utilization, fatty acid methyl ester analysis, analysis of recA gene sequences, and DNA-DNA hybridization. The results from these studies indicated that the strains CBMB40, CBPB-HIM, and CBPBHOD can be assigned under Burkholderia vietnamiensis, Burkholderia ubonensis, and Burkholderia pyrrocinia, respectively.
Madhaiyan
Munusamy
M
Department of Agricultural Chemistry, Chungbuk National University, Cheongju 361-763, Korea.
Poonguzhali
Selvaraj
S
Kwon
Soon-Wo
SW
Song
Myung-Hee
MH
Sa
Tongmin
T
D019117:Burkholderia / Q000145:classification / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D020459:Genes, rRNA; D009693:Nucleic Acid Hybridization; D012275:Oryza / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S