Identification and characterization of N-glycosylated proteins using proteomics.
Glycoproteins constitute a large fraction of the proteome. The fundamental role of protein glycosylation in cellular development, growth, and differentiation, tissue development, and in host-pathogen interactions is by now widely accepted. Proteome-wide characterization of glycoproteins is a complex task and is currently achieved by mass spectrometry-based methods that enable identification of glycoproteins and localization, classification, and analysis of individual glycan structures on proteins. In this chapter we briefly introduce a range of analytical technologies for recovery and analysis of glycoproteins and glycopeptides. Combinations of affinity-enrichment techniques, chemical and biochemical protocols, and advanced mass spectrometry facilitate detailed glycoprotein analysis in proteomics, from fundamental biological studies to biomarker discovery in biomedicine.
Selby
David S
DS
Protein Research Group, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, Denmark.
Larsen
Martin R
MR
Calvano
Cosima Damiana
CD
Jensen
Ole Nørregaard
ON
D006023:Glycoproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D006031:Glycosylation; D037102:Lectins / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods; D010455:Peptides / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D011499:Protein Processing, Post-Translational; D040901:Proteomics / Q000379:methods*
D006023:Glycoproteins; D037102:Lectins; D010455:Peptides