Repair of UVB-damaged skin by the antioxidant sulphated flavone glycoside thalassiolin B isolated from the marine plant Thalassia testudinum Banks ex König.
Daily topical application of the aqueous ethanolic extract of the marine sea grass, Thalassia testudinum, on mice skin exposed to UVB radiation resulted in a dose-dependent recovery of the skin macroscopic alterations over a 6-day period. Maximal effect (90%) occurred at a dose of 240 microg/cm(2), with no additional effects at higher doses. Bioassay-guided fractionation of the plant extract resulted in the isolation of thalassiolin B (1). Topical application of 1 (240 microg/cm(2)) markedly reduces skin UVB-induced damage. In addition, thalassiolin B scavenged 2,2-diphenyl-2-picrylhydrazyl radical with an EC(50) = 100 microg/ml. These results suggest that thalassiolin B is responsible for the skin-regenerating effects of the crude extract of T. testudinum.
Regalado
Erik L
EL
Department of Chemistry, Center of Marine Bioproducts, Loma y 37, Alturas del Vedado, C.P. 10400 Havana, Cuba. erikluis18@gmail.com
Rodríguez
María
M
Menéndez
Roberto
R
Concepción
Angel A
AA
Nogueiras
Clara
C
Laguna
Abilio
A
Rodríguez
Armando A
AA
Williams
David E
DE
Lorenzo-Luaces
Patricia
P
Valdés
Olga
O
Hernandez
Yasnay
Y
D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D031321:Hydrocharitaceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D011833:Radiation Injuries, Experimental / Q000188:drug therapy; D012867:Skin / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
D000975:Antioxidants; D005419:Flavonoids; C471629:thalassiolin B