title: Mushroom acidic glycosphingolipid induction of cytokine secretion from murine T cells and proliferation of NK1.1 alpha/beta TCR-double positive cells in vitro.
abstract: Interferon (IFN)-gamma and interleukin (IL)-4 regulate many types of immune responses. Here we report that acidic glycosphingolipids (AGLs) of Hypsizigus marmoreus and Pleurotus eryngii induced secretion of IFN- gamma and IL-4 from T cells in a CD11c-positive cell-dependent manner similar to that of alpha-galactosylceramide (alpha-GalCer) and isoglobotriaosylceramide (iGb3), although activated T cells by AGLs showed less secretion of cytokine than those activated by alpha-GalCer. In addition, stimulation of these mushroom AGLs induced proliferation of NK1.1 alpha/beta TCR-double positive cells in splenocytes. Administration of a mixture of alpha-GalCer and AGLs affected the stimulation of alpha-GalCer and generally induced a subtle Th1 bias for splenocytes but induced an extreme Th2 bias for thymocytes. These results suggested that edible mushroom AGLs contribute to immunomodulation.
authors.affiliation: Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Nozaki
authors.forename: Saki
authors.initials: S
authors.lastname: Itonori
authors.forename: Mutsumi
authors.initials: M
authors.lastname: Sugita
authors.forename: Kimihide
authors.initials: K
authors.lastname: Nakamura
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Ohba
authors.forename: Akemi
authors.initials: A
authors.lastname: Suzuki
authors.forename: Yasunori
authors.initials: Y
authors.lastname: Kushi
mesh_terms: D020384:Acidic Glycosphingolipids / Q000494:pharmacology*; D000363:Agaricales / Q000737:chemistry*; D000818:Animals; D000950:Antigens, Ly; D000954:Antigens, Surface / Q000032:analysis; D049109:Cell Proliferation; D016207:Cytokines / Q000378:metabolism*; D007155:Immunologic Factors / Q000494:pharmacology*; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D037181:Lectins, C-Type / Q000032:analysis; D008213:Lymphocyte Activation; D051379:Mice; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011948:Receptors, Antigen, T-Cell / Q000032:analysis; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology
chemical_list: D020384:Acidic Glycosphingolipids; D000950:Antigens, Ly; D000954:Antigens, Surface; D016207:Cytokines; D007155:Immunologic Factors; C527211:Klrb1c protein, mouse; D037181:Lectins, C-Type; D055651:NK Cell Lectin-Like Receptor Subfamily B; D011948:Receptors, Antigen, T-Cell; D015847:Interleukin-4; D007371:Interferon-gamma