IkappaBalpha is required for marginal zone B cell lineage development.
Inactivation of members of the nuclear factor-kappaB (NF-kappaB) family results in the decrease or defect of marginal zone B (MZB) cells. It is not known which inhibitors of the NF-kappaB family (IkappaB) are required for MZB cell development. Here, we show that mice with B cell-specific inactivation of the main NF-kappaB inhibitor IkappaBalpha have a marked decrease of MZB cells and their presumed precursors. They exhibited increased mortality rates after blood-borne bacterial infection, indicating the importance of MZB cells for bacterial clearance. In contrast, response to T cell-dependent and -independent antigens resulted only in minor changes in immunoglobulin production. Our data demonstrate the importance of the intact NF-kappaB/IkappaBalpha pathway for proper MZB cell development.
Ellinghaus
Ursula
U
Department of Hematology and Oncology, Charité, Campus Virchow-Klinikum, University Medicine Berlin, Berlin, Germany.
Rupec
Rudolf A
RA
Pabst
Oliver
O
Ignatius
Ralf
R
Förster
Reinhold
R
Dörken
Bernd
B
Jundt
Franziska
F
D000818:Animals; D001402:B-Lymphocytes / Q000502:physiology*; D019070:Cell Lineage; D020672:I-kappa B Proteins / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D000072000:NF-KappaB Inhibitor alpha; D051883:Receptor, Notch2 / Q000502:physiology; D015398:Signal Transduction
D020672:I-kappa B Proteins; C000606205:Nfkbia protein, mouse; C497765:Notch2 protein, mouse; D051883:Receptor, Notch2; D000072000:NF-KappaB Inhibitor alpha