Investigation of biosensor signal bioamplification: comparison of direct electrochemistry phenomena of individual Laccase, and dual Laccase-Tyrosinase copper enzymes, at a Sonogel-Carbon electrode.
Direct electrochemistry of Trametes versicolor Laccase (LAC) was found at a Sonogel-Carbon electrode. The bioamplification, performed by dual immobilization of this enzyme and Mushroom Tyrosinase (TYR), of the bio-electrocatalytic reduction of O(2) was investigated. The calculated alpha transfer coefficients were 0.64 and 0.67, and the heterogeneous electron-transfer rate constants were 6.19 and 8.52 s(-1), respectively, for the individual LAC and dual LAC-TYR-based Nafion/Sonogel-Carbon bio-electrodes. The responses of the dual enzymes electrode to polyphenols were stronger than those of the individual LAC or TYR biosensors. Hypotheses are offered about the mechanism of bioamplification. The surfaces of the biosensors were also characterized by AFM.
ElKaoutit
Mohammed
M
Departamento de Quimica Analitica, Facultad de Ciencias, Universidad de Cadiz, Apdo. 40, 11510 Puerto Real, Cadiz, Spain.
Naranjo-Rodriguez
Ignacio
I
Temsamani
Khalid Riffi
KR
Domínguez
Manuel
M
Hidalgo-Hidalgo de Cisneros
Jose Luis
JL
D000465:Algorithms; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000737:chemistry*; D002384:Catalysis; D003300:Copper / Q000737:chemistry*; D004563:Electrochemistry; D004566:Electrodes; D004579:Electron Transport; D004800:Enzymes, Immobilized; D007700:Kinetics; D042845:Laccase / Q000737:chemistry*; D018625:Microscopy, Atomic Force / Q000379:methods; D014442:Monophenol Monooxygenase / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000737:chemistry; D015203:Reproducibility of Results; D013499:Surface Properties
D004800:Enzymes, Immobilized; D002244:Carbon; D003300:Copper; D042845:Laccase; D014442:Monophenol Monooxygenase; D010100:Oxygen