Studies on a wild strain of Schizophyllum commune: Cellulase and xylanase production and formation of the extracellular polysaccharide Schizophyllan.
A wild strain of schizophyllum commune (Fr:Fr:) isolated in Bangladesh produced cellulase and xyianase in high yields as well as the exobiopolymer schizophyllan. It was found experimentally that concentrations of 4% Avicel, 3.5% peptone, and 0.5% Ca(NO(3))(2).4H(2)O were optimal for growth and product formation. Bacto-peptone was found to be the most suitable substrate of a number of casein, mycological, and meat peptone preparations for enzyme production. Young plate-culture inocula (4 days) were found to be better than comparatively aged fungal cultures (14 days). With the optimized medium, 5 units filter paper (FP) cellulase, 1244 units xylanase, 108 units beta-glucosidase, and 65 units of carboxymethyl (CM) cellulase per mL culture filtrate were obtained in shake flasks. In a laboratory fermentor the respective enzyme activities were 4.5 units FP-cellulase, 1200 units xylanase, 100 units beta-glucosidase, and 60 units CM-cellulase per mL culture filtrate. A biopolymer, reported to be active against can cerous cells, was an additional product in addition to the enzymes.
Steiner
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W
Institut für Biotechnologie, Mikrobiologie und Abfalltechnologie, Technische Universität, A-8010 Graz, Schlögelgasse 9/II. St., Austria.
Lafferty
R M
RM
Gomes
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I
Esterbauer
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