Oxygen uptake by entrapped hybridoma cells.
Oxygen consumption by hybridoma cells immobilized in 1- and 3.9-mm-diameter calcium alginate beads was measured. The entrapped cells consumed oxygen at about 10 micromol/min per 10(9) cells, regardless of the bead size and cell loading. In contrast, the same cells in suspension culture respire at specific rates of 3-8 micromol/min per 10(9) cells (depending on the cell density). The growth rate of the immobilized cells was significantly reduced, while specific antibody production was comparable to that of free cells.
Wohlpart
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Center for Bioprocess Development, Department of Chemical Engineering, University of Virginia, Charlottesville, Virginia 22903-2442, USA.
Gainer
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J
Kirwan
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