Identification and characterization of two mtf-1 genes in common carp.
The Metal-responsive Transcription Factor (MTF-1) serves as an essential regulator of Zn(2+) homeostasis via the activation of metallothionein gene expression. Only a single mtf-1 gene has been identified in any organism investigated previously. We report here the first evidence of the existence of two genes encoding MTF-1 proteins (mtf-1.1 and mtf-1.2). The expression patterns were followed in the liver, kidney, muscle, brain and heart by means of Northern hybridization and reverse transcription coupled polymerase chain reactions (RT-PCR). mtf-1.1 mRNA was detected in all tissues examined, with the highest level in the brain, and the lowest in the kidney and the liver. mtf-1.2 expression was detected exclusively in the brain. Cold shock and Cd(2+) exposure influence the gene expression at the transcriptional level, in a stress-specific manner.
Ferencz
Agnes
A
Department of Biochemistry and Molecular Biology, Faculty of Science, University of Szeged, P.O. Box 533, H-6701 Szeged, Hungary.
Hermesz
Edit
E
D000595:Amino Acid Sequence; D000818:Animals; D001921:Brain / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity; D002347:Carps / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D005796:Genes / Q000235:genetics*; D007035:Hypothermia / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D014157:Transcription Factors / Q000235:genetics*; D015027:Zebrafish / Q000235:genetics; D000097809:Transcription Factor MTF-1
D002104:Cadmium; D004268:DNA-Binding Proteins; D014157:Transcription Factors; D000097809:Transcription Factor MTF-1