title: Expression of the ethylene response factor gene TSRF1 enhances abscisic acid responses during seedling development in tobacco.
abstract: Ethylene response factor (ERF) proteins function as multiple regulators in the interaction of different stress-responsive pathways. During investigating the interaction of ethylene and abscisic acid (ABA) pathways, several GCC-box-binding repressors of ERF proteins have been reported to repress both ethylene- and ABA-related responses, but it is unclear how GCC-box-binding activator ERF proteins are involved in this interaction. Previously, we isolated an ERF protein tomato stress-responsive factor 1 (TSRF1) from tomato by yeast one hybrid, and showed that TSRF1 as a transcriptional activator physically interacts with GCC box, and activates the expression of GCC box-containing genes and enhances resistance to pathogens, while ABA treatment alters the binding ability of TSRF1 with this element and decreases resistance to pathogen Ralstonia solanacearum. Here, we further report that TSRF1 is able to interact with a GCC box-like sequence (indicated as CE1/GCC in this paper) containing the core sequence of ZmABI4-binding-CE1-like element, and regulates ABA responses. Overexpression of TSRF1 in tobacco enhances ABA sensitivity during germination, cotyledon expansion and root elongation. Biochemical and molecular analyses demonstrate that TSRF1 interacts with CE1/GCC. Importantly, ABA treatment enhances the interaction of TSRF1 with the ABA-responsive element and subsequently increasing the expression of ABA-responsive or CE1/GCC-containing genes. In addition, TSRF1 also promotes the expression of senescence-associated genes and tobacco seedling senescence in response to ABA. These results show that TSRF1, a GCC-box-binding activator in plant pathogen resistance, positively regulates ABA-related plant developmental processes.
authors.affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Zhang
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Yang
authors.forename: Zhijin
authors.initials: Z
authors.lastname: Zhang
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Xue-Chen
authors.initials: XC
authors.lastname: Wang
authors.forename: Rongfeng
authors.initials: R
authors.lastname: Huang
mesh_terms: D000040:Abscisic Acid / Q000494:pharmacology*; D018548:Cotyledon / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D005030:Ethylenes / Q000494:pharmacology; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D018551:Solanum lycopersicum / Q000235:genetics; D010937:Plant Growth Regulators / Q000494:pharmacology; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D018517:Plant Roots / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D030821:Plants, Genetically Modified / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development; D020133:Reverse Transcriptase Polymerase Chain Reaction; D036226:Seedlings / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D012639:Seeds / Q000187:drug effects / Q000235:genetics / Q000254:growth & development; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators; D010940:Plant Proteins; D000040:Abscisic Acid; C036216:ethylene