Effects of Himatanthus lancifolius on human leukocyte chemotaxis and their adhesion to integrins.
The aim of this work was to investigate the anti-inflammatory activities of the uleine-rich fraction extracted from the barks of Himatanthus lancifolius (Muell. Arg.) Woodson (Apocynaceae). To achieve this, we focused on its in vitro effects on some steps of the inflammatory response using peripheral human leukocytes. The results presented herein show that the uleine-rich fraction significantly inhibits the migration of casein-induced granulocytes and their adhesion to fibronectin and vitronectin, along with mononuclear cells, by down-regulating the expression of alpha 4beta1 and alpha5beta1 integrins. The data suggest that H. LANCIFOLIUS has the potential of interferring with leukocyte trafficking through its uleine-rich fraction, emphasizing its usefulness in inflammatory conditions. DEXA:dexamethasone disodium phosphate FN:fibronectin PMN:polymorphonuclear URF:uleine-rich fraction VN:vitronectin.
Nardin
Jeanine Marie
JM
Pharmaceutical Sciences Post-graduation Program, Federal University of Paraná, Curitiba, Brazil.
de Souza
Wesley Maurício
WM
Lopes
Juliano Ferreira
JF
Florão
Angela
A
de Moraes Santos
Cid Aimbiré
CA
Weffort-Santos
Almeriane Maria
AM
D000470:Alkaloids / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D029062:Apocynaceae / Q000737:chemistry*; D001952:Bridged-Ring Compounds / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D002364:Caseins / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D054441:Cell Migration Assays, Leukocyte; D002633:Chemotaxis / Q000187:drug effects*; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D039041:Integrin alpha4beta1 / Q000378:metabolism; D039081:Integrin alpha5beta1 / Q000378:metabolism; D007962:Leukocytes / Q000187:drug effects*; D008517:Phytotherapy; D024301:Plant Bark / Q000737:chemistry; D010936:Plant Extracts / Q000494:pharmacology / Q000627:therapeutic use; D019096:Vitronectin / Q000378:metabolism
D000470:Alkaloids; D001952:Bridged-Ring Compounds; D002364:Caseins; D005353:Fibronectins; D039041:Integrin alpha4beta1; D039081:Integrin alpha5beta1; D010936:Plant Extracts; D019096:Vitronectin; C428545:uleine