Glutathione peroxidase 1-deficient mice are more susceptible to doxorubicin-induced cardiotoxicity.
Doxorubicin (DOX)-induced cardiotoxicity is thought to be mediated by the generation of superoxide anion radicals (superoxide) from redox cycling of DOX in cardiomyocyte mitochondria. Reduction of superoxide generates H(2)O(2), which diffuses throughout the cell and potentially contributes to oxidant-mediated cardiac injury. The mitochondrial and cytosolic glutathione peroxidase 1 (Gpx1) primarily functions to eradicate H(2)O(2). In this study, we hypothesize that Gpx1 plays a pivotal role in the clearance of H(2)O(2) generated by DOX. To test this hypothesis, we compared DOX-induced cardiac dysfunction, mitochondrial injury, protein nitration, and apoptosis in Gpx1-deficient and wild type mouse hearts. The Gpx1-deficient hearts showed increased susceptibility to DOX-induced acute functional derangements than wild type hearts, including impaired contractility and diastolic properties, decreased coronary flow rate, and reduced heart rate. In addition, DOX treatment impaired the mitochondrial function of Gpx1-deficient hearts. Specifically, Gpx1-deficient hearts treated with DOX demonstrated an increased rate of NAD-linked state 4 respiration and a decline in the P/O ratio relative to wild type hearts, suggesting that DOX uncouples the electron transfer chain and oxidative phosphorylation in Gpx1-deficient hearts. Finally, apoptosis and protein nitration were significantly increased in Gpx1-deficient mouse hearts compared to wild type hearts. These studies suggest that Gpx1 plays significant roles in protecting DOX-induced mitochondrial impairment and cardiac dysfunction in the acute phase.
Gao
Jinping
J
Cecile Cox Quillen Laboratory of Geriatric Research, James Quillen College of Medicine, East Tennessee State University, James H. Quillen Veterans Affairs Medical Center, Johnson City, TN 37614, USA.
Xiong
Ye
Y
Ho
Ye-Shih
YS
Liu
Xuwan
X
Chua
Chu Chang
CC
Xu
Xingshun
X
Wang
Hong
H
Hamdy
Ronald
R
Chua
Balvin H L
BH
D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D004317:Doxorubicin / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000172:deficiency* / Q000235:genetics / Q000378:metabolism*; D006331:Heart Diseases / Q000139:chemically induced* / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D051379:Mice; D018345:Mice, Knockout; D008928:Mitochondria / Q000187:drug effects / Q000201:enzymology; D009584:Nitrogen / Q000378:metabolism; D000094765:Glutathione Peroxidase GPX1
D004317:Doxorubicin; D005979:Glutathione Peroxidase; D009584:Nitrogen; D000094765:Glutathione Peroxidase GPX1; C000722068:Gpx1 protein, mouse