[Study of flavonoids distribution in various populations of Prunella vulgaris].
OBJECTIVE
To study the extraction flavonoids distribution in different parts of Prunella vulgaris from different populations.


METHODS
The optimal extraction condition was selected by response surface method (RSM). The amounts of flavonoids were determined by colorimetric method.


RESULTS
The optimum extraction condition was using 35% ethanol at 25 times of sawple volume, refluxing at 87 degrees C for 3.5 h. The amount of flavonoids was 2.16% -10.29% in P. vulgaris. The content of flavonoids was the highest in leaf while that in root was the lowest. And the content of flavonoids in spike after removed seeds was 27.6% higher than the one reserved seeds.


CONCLUSIONS
The RSM was feasible for optimum extraction condition and the amount of flavonoids showed a signiticant regional distribution pattern in various populations of P. vulgaris.
Liao
Li
L
Institute of Chinese Medicinal Materials, Nanjin Agricultural University, Nanjing 210095, China.
Guo
Qiao-Sheng
QS
Liu
Li
L
Liu
Min
M
D004365:Drugs, Chinese Herbal / Q000378:metabolism; D005240:Feasibility Studies; D005419:Flavonoids / Q000302:isolation & purification / Q000378:metabolism*; D018514:Plant Structures / Q000378:metabolism; D031347:Prunella / Q000378:metabolism*
D004365:Drugs, Chinese Herbal; D005419:Flavonoids