title: Selective separation and purification of two lipases from chromobacterium viscosum using AOT reversed micelles.
abstract: Selective separation and purification of two lipases form Chromobacterium viscosum were carried out by liquid-liquid extraction using a reversed micellar system. Optimum parameters for extraction were determined using a 250 mM AOT micellar solution in isooctane. Complete separation of the two lipases was achieved at pH 6.0 with a 50mM potassium phosphate buffer solution containing 50 mM KCI. By adding 2.5% by volume of ethanol to the lipase-loaded micellar solution, 85% of the extracted lipase could be recovered in a new aqueous phase, 50 mM K(2)HPO(4) with 50 mM KCl, at pH 9.0. Lipase A was purified 2.6-fold with a recovery of 86%, and lipase B by 1.5-fold with a recovery of 76%.
authors.affiliation: Laboratório de Engenharia Bioqúimica, Instituto Superior Técnico, 1000 Lisboa, Portugal.
authors.forename: M R
authors.initials: MR
authors.lastname: Aires-Barros
authors.forename: J M
authors.initials: JM
authors.lastname: Cabral