title: Synthesis of a novel fluorescent probe based on acridine skeleton used for sensitive determination of DNA.
abstract: In this study, a novel fluorescent probe of acridine derivative N-((N-(2-dimethylamino)ethyl)acridine-4-carboxamide)-alpha-alanine (N-(ACR-4-CA)-alpha-ALA) was synthesized. The structure of the new compound was characterized by (1)H NMR, MS, elemental analysis, fluorescent and ultraviolet spectra. It was found that DNA had the ability to quench the fluorescence of N-(ACR-4-CA)-alpha-ALA, and the quenched intensity of fluorescence was proportional to the concentration of DNA. A method for DNA determination based on the quenching fluorescence (lambda(ex) = 260 nm, lambda(em) = 451 nm) of N-(ACR-4-CA)-alpha-ALA was established. Under optimal conditions, the linear range is 0.05-2.0 microg mL(-1) for both fish semen (fsDNA) and calf thymus DNA (ctDNA). The corresponding determination limits are 9.1 ng mL(-1) for fsDNA and 8.7 ng mL(-1) for ctDNA, respectively. The results suggested that the interaction mode between N-(ACR-4-CA)-alpha-ALA and DNA was intercalative binding. The intrinsic binding constant was determined and the result showed a large binding constant of N-(ACR-4-CA)-alpha-ALA with DNA.
authors.affiliation: Department of Chemistry, Fuzhou University, Fuzhou 350002, China.
authors.forename: Menghui
authors.initials: M
authors.lastname: Wu
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Wu
authors.forename: Xi
authors.initials: X
authors.lastname: Gao
authors.forename: Xucong
authors.initials: X
authors.lastname: Lin
authors.forename: Zenghong
authors.initials: Z
authors.lastname: Xie
mesh_terms: D000166:Acridines / Q000138:chemical synthesis / Q000737:chemistry*; D000409:Alanine / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry; D000818:Animals; D002138:Calibration; D002417:Cattle; D004247:DNA / Q000032:analysis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D011506:Proteins / Q000737:chemistry; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence; D013997:Time Factors
chemical_list: D000166:Acridines; D005456:Fluorescent Dyes; C532498:N-((N-(2-dimethylamino)ethyl)acridine-4-carboxamide)-alpha-alanine; D011506:Proteins; D004247:DNA; D000409:Alanine