title: Establishment of the comet assay in the freshwater snail Biomphalaria glabrata (Say, 1818).
abstract: The single cell gel electrophoresis or the comet assay was established in the freshwater snail Biomphalaria glabrata. For detecting DNA damage in circulating hemocytes, adult snails were irradiated with single doses of 2.5, 5, 10 and 20 Gy of (60)Co gamma radiation. Genotoxic effect of ionizing radiation was detected at all doses as a dose-related increase in DNA migration. Comet assay in B. glabrata demonstrated to be a simple, fast and reliable tool in the evaluation of genotoxic effects of environmental mutagens.
authors.affiliation: Laboratório de Parasitologia/Malacologia, Instituto Butantan, Avenida Vital Brasil, 1500, CEP 05503-900, São Paulo, Brazil.
authors.forename: Vanessa Siqueira
authors.initials: VS
authors.lastname: Grazeffe
authors.forename: Lenita de Freitas
authors.initials: Lde F
authors.lastname: Tallarico
authors.forename: Alessandro de Sá
authors.initials: Ade S
authors.lastname: Pinheiro
authors.forename: Toshie
authors.initials: T
authors.lastname: Kawano
authors.forename: Miriam Fussae
authors.initials: MF
authors.lastname: Suzuki
authors.forename: Kayo
authors.initials: K
authors.lastname: Okazaki
authors.forename: Carlos Alberto de Bragança
authors.initials: CA
authors.lastname: Pereira
authors.forename: Eliana
authors.initials: E
authors.lastname: Nakano
mesh_terms: D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001700:Biomphalaria / Q000235:genetics / Q000528:radiation effects; D020552:Comet Assay; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D004784:Environmental Monitoring / Q000379:methods*; D005720:Gamma Rays; D006458:Hemolymph / Q000528:radiation effects; D012680:Sensitivity and Specificity