Fed-batch fermentation for the production of alpha-amylase by Bacillus amyloliquefaciens.
Fed-batch cultures were performed to maximize the alpha-amylase activity in a bioreactor. Kinetic equations containing a catabolite repression effect were used to model the enzyme formation from Bacillus amyloliquefaciens. Fed-batch culture experiments were performed using maltose to implement the optimal feeding strategy. Optimal fed-batch culture based on sequential parameter estimation was performed successfully using off-line analysis while the fermentation was in progress. The enzyme activity from the fed-batch culture employing maltose was higher than that of the batch culture by 60%. Enzyme production using starch showed similar trends to those obtained using maltose.
Yoo
Y J
YJ
Department of Chemical and Nuclear Engineering, University of Maryland, College Park, Maryland 20742.
Cadman
T W
TW
Hong
J
J
Hatch
R T
RT