title: Macroporous and hydrophilic polymer resins modified with isothiocyanate groups for immobilization of enzymes.
abstract: Hydrophilic and macroporous polymer resins composed of glycerylmethacrylate, styrene, and divinylbenzene were quite easily modified with isothiocyanate groups using a Friedel-Crafts reaction with 3-chloropropionyl chloride and subsequent nucleophilic reaction with KSCN. Alkaline phosphatase and trypsin could be covalently bound to the isothiocyanate-carrying polymer resins, and the immobilized enzymes obtained were sufficiently active and stable due to a covalent bonding via a spacer group between the carrier resins and the enzymes and also due to a hydrophilic environment around the enzymes. A heterogeneity of the immobilized enzyme was taken into account to interpret the thermal denaturation process of the enzyme immobilized onto the resins.
authors.affiliation: Department of Polymer Chemistry, Kyoto University, Kyoto, Japan.
authors.forename: M
authors.initials: M
authors.lastname: Hasegawa
authors.forename: H
authors.initials: H
authors.lastname: Kitano
authors.forename: R
authors.initials: R
authors.lastname: Nishida
authors.forename: T
authors.initials: T
authors.lastname: Kobashi