Orthogonal electrophoretic fractionation of DNA in agarose gels.
We developed an electrophoretic procedure, using Voltage Gradient Gel Electrophoresis (VGGE), which allows to obtain both an improvement of the resolution power of the system in orthogonal fractionation of DNA and, mainly, an about fourfold enhancement of hybridization signals in Southern blotting applications.
Bellavia
Daniele
D
Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Palermo, V.le delle Scienze, Edificio 16, Palermo, Italy.
Sisino
Giorgia
G
Sisino
Giada
G
Barbieri
Rainer
R
D004247:DNA / Q000032:analysis*; D004587:Electrophoresis, Agar Gel / Q000379:methods*; D016753:Interleukin-10 / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics
D012333:RNA, Messenger; D016753:Interleukin-10; D004247:DNA