External polyacrylate-coating as alternative material for preparation of photopolymerized sol-gel monolithic column.
Photopolymerized sol-gel monolithic columns for use in capillary electrochromatography were prepared in 125 microm i.d. polyacrylate-coated fused-silica capillaries. The polyacrylate-coating, unlike the polyimide one, is transparent to the radiation used (approximately 370 nm), and thus, no coating removal is necessary. This is a very important particularity since intrinsic capillary column characteristics, such as flexibility and mechanical resistance, are unchanged. A mixture containing metacryloxypropyltrimethoxysilane (MPTMS) as the polymeric precursor, hydrochloric acid as the catalyst, toluene as the porogen and bis(2,4,6-trimethylbenzoyl)-phenylphosphine oxide (Irgacure 819) as the photoinitiator was irradiated at 370 nm for 20 min inside the capillaries to prepare the columns through sol-gel approach. The versatility and viability of the use of polyacrilate as a new capillary external coating were shown through preparation of two columns under different conditions, which were tested in electrochromatography for separation of standard mixture containing thiourea (marker compound), propylbenzene, phenanthrene and pyrene.
Vaz
Fernando Antonio Simas
FA
Departamento de Química, Universidade Federal de Juiz de Fora, UFJF, 36036-330 Juiz de Fora, MG, Brazil.
de Castro
Patrícia Mendonça
PM
Molina
Celso
C
Ribeiro
Sidney José Lima
SJ
Polachini
Ferminio César
FC
Messaddeq
Younes
Y
Nunes
Adriana Palombo
AP
de Oliveira
Marcone Augusto Leal
MA