Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor.
A mismatch amplification mutation PCR assay was developed and validated for rapid detection of the biotype specific cholera toxin B subunit of V. cholerae O1. This assay will enable easy monitoring of the spread of a new emerging variant of the El Tor biotype of V. cholerae O1.
Morita
Masatomo
M
National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo, 162-8640, Japan.
Ohnishi
Makoto
M
Arakawa
Eiji
E
Bhuiyan
N A
NA
Nusrin
Suraia
S
Alam
Munirul
M
Siddique
A K
AK
Qadri
Firdausi
F
Izumiya
Hidemasa
H
Nair
G Balakrish
GB
Watanabe
Haruo
H
D000483:Alleles; D015373:Bacterial Typing Techniques / Q000379:methods*; D002772:Cholera Toxin / Q000235:genetics*; D017931:DNA Primers; D005240:Feasibility Studies; D005798:Genes, Bacterial / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods*; D041241:Vibrio cholerae O1 / Q000145:classification* / Q000235:genetics*
D017931:DNA Primers; D002772:Cholera Toxin