title: Pre-analytical method for metabolic profiling of plant cell cultures of Passiflora garckei.
abstract: Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparation method. Solid phase extraction (SPE) was employed to isolate the plant metabolites for nuclear magnetic resonance (NMR) analysis and to subsequently detect the differences between yeast extract elicited and control cells. Compared with previous results obtained by using a Sephadex LH-20 column, SPE coupled with NMR spectroscopy improves the analysis of aromatic compounds e.g.: trans-feruloyl derivatives and trans-coumaroyl derivatives. Moreover, it decreases the concentration of sugars that usually overlap with many plant metabolite signals.
authors.affiliation: Scientific and Technological Department, Faculty of Mathematics, Physics and Natural Science, University of Verona, Verona, Italy. maricafraccaroli@gmail.com
authors.forename: Marica
authors.initials: M
authors.lastname: Fraccaroli
authors.forename: Silvia
authors.initials: S
authors.lastname: Nicoletti
authors.forename: Federica
authors.initials: F
authors.lastname: Maltese
authors.forename: Young Hae
authors.initials: YH
authors.lastname: Choi
authors.forename: Flavia
authors.initials: F
authors.lastname: Guzzo
authors.forename: Marisa
authors.initials: M
authors.lastname: Levi
authors.forename: Robert
authors.initials: R
authors.lastname: Verpoorte
mesh_terms: D002457:Cell Extracts / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification*; D002478:Cells, Cultured; D009682:Magnetic Resonance Spectroscopy; D000432:Methanol / Q000737:chemistry; D029598:Passiflora / Q000166:cytology / Q000378:metabolism*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D052616:Solid Phase Extraction
chemical_list: D002457:Cell Extracts; D000432:Methanol