title: Reactive oxidant species and oxidation of protein and haemoglobin as biomarkers of susceptibility to stress caused by chloramphenicol.
abstract: Chemiluminescence assay was suitable for rapid determination of alterations caused by chloramphenicol (Ch) in blood. The new aspects observed indicated oxidative stress with: (a) biphasic response of reactive oxidant species (ROS) with different concentration of Ch; (b) increase of carbonyl residues and advanced oxidation protein products in blood as a consequence of oxidative stress; (c) oxidation of haemoglobin with rise of oxyhaemoglobin; protein and haemoglobin oxidation was more pronounced in the samples that exhibited high stimuli of ROS; and (d) the ferrous reduction antioxidant potential (FRAP) was the lowest in the maximum ROS-producer sample, which exhibited the lowest consumption of FRAP in response to Ch treatment. The present investigation could contribute to a rapid detection of persons with elevated susceptibility to Ch previously to its application in chemotherapy.
authors.affiliation: Department of Pharmacy, Faculty of Chemical Sciences, National University of Córdoba, Haya de la Torre y Medina Allende, University Campus, 5000 Córdoba, Argentina.
authors.forename: Viviana
authors.initials: V
authors.lastname: Correa-Salde
authors.forename: Ines
authors.initials: I
authors.lastname: Albesa
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000633:toxicity*; D015415:Biomarkers / Q000378:metabolism; D002701:Chloramphenicol / Q000008:administration & dosage / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D006454:Hemoglobins / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D066298:In Vitro Techniques; D008163:Luminescent Measurements / Q000379:methods*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D010108:Oxyhemoglobins / Q000187:drug effects / Q000378:metabolism; D011506:Proteins / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism*
chemical_list: D000900:Anti-Bacterial Agents; D015415:Biomarkers; D006454:Hemoglobins; D010108:Oxyhemoglobins; D011506:Proteins; D017382:Reactive Oxygen Species; D002701:Chloramphenicol