title: Lactose hydrolysis by immobilized beta-galactosidase in capillary bed reactor.
abstract: The hydrolysis of lactose by immobilized beta-galactosidase was studied in a continuous-flow capillary bed reactor operating at 30 degrees C. Solutions containing 50, 100, and 150 g lactose and 0.5 g sodium acetate/L were fed to the reactor. Lactose conversions ranging from 24% to greater than 99% were achieved at reactor space times ranging from 0.06 to 6.3 min. These conversion data were successfully modeled in terms of a plug flow reactor model and a form of Michaelis-Menten kinetics which included competitive inhibition by both the alpha and beta forms of galactose.
authors.affiliation: Department of Chemical Engineering, University of Wisconsin, Madison, Wisconsin 53706, USA.
authors.forename: R S
authors.initials: RS
authors.lastname: Peterson
authors.forename: C G
authors.initials: CG
authors.lastname: Hill
authors.forename: C H
authors.initials: CH
authors.lastname: Amundson