title: Turnip mosaic virus VPg interacts with Arabidopsis thaliana eIF(iso)4E and inhibits in vitro translation.
abstract: The interaction between turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) and Arabidopsis thaliana eukaryotic initiation factor (iso)4E (eIF(iso)4E) was investigated to address the influence of potyviral VPg on host cellular translational initiation. Affinity chromatographic analysis showed that the region comprising amino acids 62-70 of VPg is important for the interaction with eIF(iso)4E. In vitro translation analysis showed that the addition of VPg significantly inhibited translation of capped RNA in eIF(iso)4E-reconstituted wheat germ extract. This result indicates that VPg inhibits cap-dependent translational initiation via binding to eIF(iso)4E. The inhibition by VPg of in vitro translation of RNA with wheat germ extract did not depend on RNase activity. Our present results may indicate that excess VPg produced at the encapsidation stage shuts off cap-dependent translational initiation in host cells by inhibiting complex formation between eIF(iso)4E and cellular mRNAs.
authors.affiliation: Department of Microbiology, St Marianna University School of Medicine, Miyamae, Kawasaki, Japan. hmiyoshi@marianna-u.ac.jp
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Miyoshi
authors.forename: Hayato
authors.initials: H
authors.lastname: Okade
authors.forename: Shinji
authors.initials: S
authors.lastname: Muto
authors.forename: Noriko
authors.initials: N
authors.lastname: Suehiro
authors.forename: Hideki
authors.initials: H
authors.lastname: Nakashima
authors.forename: Koji
authors.initials: K
authors.lastname: Tomoo
authors.forename: Tomohide
authors.initials: T
authors.lastname: Natsuaki
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D029688:Brassica napus / Q000821:virology*; D020134:Catalytic Domain; D002846:Chromatography, Affinity; D003851:Deoxyribonucleases / Q000378:metabolism; D039642:Eukaryotic Initiation Factors / Q000378:metabolism*; D017800:Potyvirus / Q000378:metabolism*; D011485:Protein Binding; D014176:Protein Biosynthesis / Q000187:drug effects*; D012333:RNA, Messenger / Q000235:genetics; D012260:Ribonucleases / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism* / Q000494:pharmacology*
chemical_list: D029681:Arabidopsis Proteins; D039642:Eukaryotic Initiation Factors; D012333:RNA, Messenger; D014764:Viral Proteins; C511734:eIF(iso)4E protein, Arabidopsis; D003851:Deoxyribonucleases; D012260:Ribonucleases