[Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique].
OBJECTIVE
To explore the appropriate amount of template DNA for Sinofiler Kit.


METHODS
The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.


RESULTS
It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.


CONCLUSIONS
Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.
Li
Cheng-Tao
CT
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R. China. lichengtaohla@163.com
Guo
Hong
H
Lin
Yuan
Y
Liu
Yan
Y
Que
Ting-Zhi
TZ
Li
Li
L
D004247:DNA / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods*; D006197:Hair / Q000737:chemistry; D006801:Humans; D018895:Microsatellite Repeats; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
D011933:Reagent Kits, Diagnostic; D004247:DNA