title: Single molecule correlation spectroscopy in continuous flow mixers with zero-mode waveguides.
abstract: Zero-Mode Waveguides were first introduced for Fluorescence Correlation Spectroscopy at micromolar dye concentrations. We show that combining zero-mode waveguides with fluorescence correlation spectroscopy in a continuous flow mixer avoids the compression of the FCS signal due to fluid transport at channel velocities up to approximately 17 mm/s. We derive an analytic scaling relationship [equation: see text] converting this flow velocity insensitivity to improved kinetic rate certainty in time-resolved mixing experiments. Thus zero-mode waveguides make FCS suitable for direct kinetics measurements in rapid continuous flow.
authors.affiliation: Department of Physics, Princeton University, Princeton, NJ 08544, USA. dliao@princeton.edu
authors.forename: David
authors.initials: D
authors.lastname: Liao
authors.forename: Peter
authors.initials: P
authors.lastname: Galajda
authors.forename: Robert
authors.initials: R
authors.lastname: Riehn
authors.forename: Rob
authors.initials: R
authors.lastname: Ilic
authors.forename: Jason L
authors.initials: JL
authors.lastname: Puchalla
authors.forename: Howard G
authors.initials: HG
authors.lastname: Yu
authors.forename: Harold G
authors.initials: HG
authors.lastname: Craighead
authors.forename: Robert H
authors.initials: RH
authors.lastname: Austin
mesh_terms: D004867:Equipment Design; D005456:Fluorescent Dyes / Q000494:pharmacology; D007700:Kinetics; D008027:Light; D015233:Models, Statistical; D008962:Models, Theoretical; D036103:Nanotechnology / Q000379:methods; D055095:Optics and Photonics; D017785:Photons; D013050:Spectrometry, Fluorescence / Q000295:instrumentation* / Q000379:methods*; D013997:Time Factors
chemical_list: D005456:Fluorescent Dyes