[High-performance liquid chromatography-mass spectrometry for determining olmesartan in human plasma].
OBJECTIVE
To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma.


METHODS
Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C(18) column (150 mm 2.1 mm, 5 microm). Samples at 40 degrees celsius;. The mobile phase consisted of water-methanol- acetonitrile(14:60:26) with a flow rate of 0.22 ml/min.


RESULTS
The lower limit of qualification was 25 microg/L. The calibration curve was linear over the range of 25-3200 microg/L (r=0.9998), with the intra-day and inter-day RSD less than 15%.


CONCLUSIONS
The method is sensitive, rapid and suitable for the study of pharmacokinetics and bioavailability of olmesartan.
Chen
Shi-Heng
SH
Department of Pharmacy, Liling Hospital of Traditional Chinese Medicine, Liling 420000, China.
Wu
Cui-Fang
CF
Chen
Ben-Mei
BM
Pei
Qi
Q
Tan
Hong-Yi
HY
Yang
Li
L
Yang
Guo-Ping
GP
D047228:Angiotensin II Type 1 Receptor Blockers / Q000097:blood; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D006801:Humans; D007093:Imidazoles / Q000097:blood*; D013058:Mass Spectrometry / Q000379:methods; D015203:Reproducibility of Results; D013777:Tetrazoles / Q000097:blood*
D047228:Angiotensin II Type 1 Receptor Blockers; D007093:Imidazoles; D013777:Tetrazoles; C437965:olmesartan