title: PROCLEIX West Nile virus assay based on transcription-mediated amplification.
abstract: The PROCLEIX West Nile virus (WNV) assay is based on transcription-mediated amplification and is the most sensitive nucleic acid test commercially available today for blood screening. Since 2003, in the USA, the assay has been used for year-round screening of blood donations in minipools of 16 and in an individual-donation testing format, when appropriate triggering guidelines for such switch become effective. The test can be run on the semiautomated PROCLEIX modular platform or the fully-automated PROCLEIX TIGRIS System. This assay and the corresponding platforms have been developed and are manufactured by San Diego-based Gen-Probe, Inc., while they are marketed and distributed by Chiron, a Novartis business. This review covers some of the epidemiological and virological aspects of WNV, as well as clinical questions and technological assessment of the transcription-mediated amplification and alternative technologies used in WNV blood screening.
authors.affiliation: Chiron, Novartis Vaccines & Diagnostics, Emeryville, CA, USA. rainer.ziermann@novartis.com
authors.forename: Rainer
authors.initials: R
authors.lastname: Ziermann
authors.forename: Sergio Arturo
authors.initials: SA
authors.lastname: Sánchez-Guerrero
mesh_terms: D000818:Animals; D006801:Humans; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation* / Q000379:methods; D012367:RNA, Viral / Q000097:blood*; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity; D014901:West Nile Fever / Q000097:blood* / Q000453:epidemiology*; D014902:West Nile virus / Q000378:metabolism*
chemical_list: D012367:RNA, Viral; D011933:Reagent Kits, Diagnostic