title: Rat prostate tumors express cancer procoagulant, an activator of coagulation factor X.
abstract: Two common procoagulant activities associated with tumors are tissue factor and cancer procoagulant (CP), an activator of coagulation factor X. We have identified a convenient source of CP in transplanted Lobund-Wistar rat PA3 prostate tumors. CP activity was purified from 5 independent transplanted prostate tumors by column chromatography. The protein activated factor X in the absence of TF and factor VII. An antihuman CP antibody recognized rat CP in an ELISA and inactivated CP activity in a chromogenic assay. Lobund-Wistar prostate tumors may provide a convenient animal model useful in determining the role of CP in cancer development.
authors.affiliation: Medical University of Lodz, Lodz, Poland. mkamocka@iupui.edu
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Kamocka
authors.forename: Morris
authors.initials: M
authors.lastname: Pollard
authors.forename: Mark
authors.initials: M
authors.lastname: Suckow
authors.forename: Wojciech P
authors.initials: WP
authors.lastname: Mielicki
authors.forename: Elliot D
authors.initials: ED
authors.lastname: Rosen
mesh_terms: D000818:Animals; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005170:Factor X / Q000378:metabolism*; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D009363:Neoplasm Proteins; D005170:Factor X; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant