Effects of paddle impeller geometry on power input and mass transfer in small-scale animal cell culture vessels.
Process scaleup for stirred-tank animal cell cultures such as suspension and microcarrier cultures often begins at the bench scale in small spinner vessels. In order to initiate process development under the proper conditions, it is essential to know the physical conditions under which the cells are grown. In this article, power inputs and surface oxygen transfer rates to culture medium in 500-mL Corning spinner vessels were determined as a function of the impeller geometry, impeller height, and agitation speed. The results obtained indicate that power dissipation dependency differs from literature correlations and may compromise scale up at constant power input from these vessels. These results are of general utility to researchers using small-scale vessels.
Aunins
J G
JG
Department of Chemical Engineering, Massachusetts Institute of Technology, Biotechnology Process Engineering Center, Cambridge, Massachusetts 02139, USA.
Woodson
B A
BA
Hale
T K
TK
Wang
D I
DI