title: Stereoselective bioreduction of bulky-bulky ketones by a novel ADH from Ralstonia sp.
abstract: Ketones with two bulky substituents, named bulky-bulky ketones, as well as less sterically demanding ketones were successfully reduced to the corresponding optically highly enriched alcohols using a novel identified recombinant short-chain alcohol dehydrogenase RasADH from Ralstonia sp. DSM 6428 overexpressed in E. coli.
authors.affiliation: Research Centre Applied Biocatalysis c/o Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, 8010 Graz, Austria.
authors.forename: Iván
authors.initials: I
authors.lastname: Lavandera
authors.forename: Alexander
authors.initials: A
authors.lastname: Kern
authors.forename: Bianca
authors.initials: B
authors.lastname: Ferreira-Silva
authors.forename: Anton
authors.initials: A
authors.lastname: Glieder
authors.forename: Stefaan
authors.initials: S
authors.lastname: de Wildeman
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Kroutil
mesh_terms: D000426:Alcohol Dehydrogenase / Q000378:metabolism*; D002384:Catalysis; D004926:Escherichia coli / Q000201:enzymology; D007659:Ketones / Q000737:chemistry* / Q000378:metabolism*; D015394:Molecular Structure; D010084:Oxidation-Reduction; D043367:Ralstonia / Q000201:enzymology*; D013379:Substrate Specificity; D018631:ras Proteins / Q000378:metabolism
chemical_list: D007659:Ketones; D000426:Alcohol Dehydrogenase; D018631:ras Proteins