title: Intracoronary delivery of bone marrow cells to the acutely infarcted myocardium. Optimization of the delivery technique.
abstract: OBJECTIVE
  Intracoronary cell transplantation during catheter balloon inflations may be associated with adverse events. We studied the effectiveness of an alternative transplantation technique--intracoronary cell infusion.
  
  
  METHODS
  Fourteen pigs, which had survived acute myocardial infarction, were randomized into 2 treatment groups and 2 controls. Three days after infarction, 12 pigs underwent allogeneic intracoronary mononuclear bone marrow cell transplantation using either the standard technique (short-term cell injections during repeat balloon inflations, technique A, n = 6) or continuous intracoronary cell infusion without balloon inflations (technique B, n = 6). Implanted cells were stained with fluorescent dye. After transplantation, the pigs were euthanized and myocardial samples were analyzed by fluorescent microscopy.
  
  
  RESULTS
  The mean numbers of fluorescently labeled bone marrow cells in the infarction border zone, in the infarction mid-area and in the center of myocardial infarction were 84, 72 and 55 using technique A, and 29, 57 and 46 using technique B, respectively. The mean cell retention in the infarction border zone of 84 cells for technique A and 29 cells for technique B differed significantly (p = 0.034, two-tailed t test).
  
  
  CONCLUSIONS
  The continuous intracoronary cell infusion technique is a less efficient cell delivery technique as compared with the standard technique using repeat intracoronary balloon inflations.
authors.affiliation: 1st Department of Internal Medicine/Cardioangiology, St. Anna Hospital, ICRC, Masaryk University, Pekarská 53, Brno, Czech Republic. jaroslav.meluzin@fnusa.cz
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Meluzín
authors.forename: Michal
authors.initials: M
authors.lastname: Vlasín
authors.forename: Ladislav
authors.initials: L
authors.lastname: Groch
authors.forename: Jirí
authors.initials: J
authors.lastname: Mayer
authors.forename: Leos
authors.initials: L
authors.lastname: Kren
authors.forename: Petr
authors.initials: P
authors.lastname: Rauser
authors.forename: Boris
authors.initials: B
authors.lastname: Tichý
authors.forename: Ivan
authors.initials: I
authors.lastname: Hornácek
authors.forename: Jan
authors.initials: J
authors.lastname: Sitar
authors.forename: Stanislav
authors.initials: S
authors.lastname: Palsa
authors.forename: Martin
authors.initials: M
authors.lastname: Klabusay
authors.forename: Zdenek
authors.initials: Z
authors.lastname: Korístek
authors.forename: Michael
authors.initials: M
authors.lastname: Doubek
authors.forename: Sárka
authors.initials: S
authors.lastname: Pospísilová
authors.forename: Leona
authors.initials: L
authors.lastname: Lexmaulová
authors.forename: Ladislav
authors.initials: L
authors.lastname: Dusek
mesh_terms: D000818:Animals; D016026:Bone Marrow Transplantation / Q000379:methods*; D006328:Cardiac Catheterization; D002404:Catheterization; D004195:Disease Models, Animal; D005260:Female; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008297:Male; D008856:Microscopy, Fluorescence; D009203:Myocardial Infarction / Q000628:therapy*; D013552:Swine
chemical_list: D005452:Fluoresceins; D005456:Fluorescent Dyes; C069306:5-chloromethylfluorescein