A C-type lectin like-domain (CTLD)-containing protein (PtLP) from the swimming crab Portunus trituberculatus.
C-type lectins play important roles in the non-self innate immune system of invertebrates. In this study, we isolated the full-length cDNA of the C-type lectin like-domain (CTLD)-containing protein, designated PtLP, from the hepatopancreas of the swimming crab Portunus trituberculatus, one of the most common edible crabs of East Asia. The PtLP cDNA consists of 923 bp and encodes a polypeptide of 164 amino acids containing a well-conserved C-type lectin like-domain (CTLD). The deduced amino acid sequence of PtLP shows 29-36% amino acid sequence identity to other crustacean C-type lectin sequences. A phylogenetic analysis revealed that PtLP is in a large cluster together with black tiger shrimp PmAV, a gene involved in virus resistance of shrimp, and all of the C-type lectins from the various shrimps. Quantitative RT-PCR analysis showed that the PtLP mRNA was expressed highly in hepatopancreas and moderately in gills, hemocytes, and ovary of normal swimming crabs.
Kong
Hee Jeong
HJ
Biotechnology Research Institute, National Fisheries Research and Development Institute, Gijang-up, Gijang-gun, Busan, South Korea. hjkong@nfrdi.re.kr
Park
Eun-Mi
EM
Nam
Bo-Hye
BH
Kim
Young-Ok
YO
Kim
Woo-Jin
WJ
Park
Hyun-Joo
HJ
Lee
Chang Hoon
CH
Lee
Sang-Jun
SJ
D000818:Animals; D003386:Brachyura / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D037181:Lectins, C-Type / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011506:Proteins / Q000737:chemistry / Q000235:genetics*; D016415:Sequence Alignment
D018076:DNA, Complementary; D037181:Lectins, C-Type; D011506:Proteins