title: Heparan sulfate in perlecan promotes mouse atherosclerosis: roles in lipid permeability, lipid retention, and smooth muscle cell proliferation.
abstract: Heparan sulfate (HS) has been proposed to be antiatherogenic through inhibition of lipoprotein retention, inflammation, and smooth muscle cell proliferation. Perlecan is the predominant HS proteoglycan in the artery wall. Here, we investigated the role of perlecan HS chains using apoE null (ApoE0) mice that were cross-bred with mice expressing HS-deficient perlecan (Hspg2(Delta3/Delta3)). Morphometry of cross-sections from aortic roots and en face preparations of whole aortas revealed a significant decrease in lesion formation in ApoE0/Hspg2(Delta3/Delta3) mice at both 15 and 33 weeks. In vitro, binding of labeled mouse triglyceride-rich lipoproteins and human LDL to total extracellular matrix, as well as to purified proteoglycans, prepared from ApoE0/Hspg2(Delta3/Delta3) smooth muscle cells was reduced. In vivo, at 20 minutes influx of human (125)I-LDL or mouse triglyceride-rich lipoproteins into the aortic wall was increased in ApoE0/Hspg2(Delta3/Delta3) mice compared to ApoE0 mice. However, at 72 hours accumulation of (125)I-LDL was similar in ApoE0/Hspg2(Delta3/Delta3) and ApoE0 mice. Immunohistochemistry of lesions from ApoE0/Hspg2(Delta3/Delta3) mice showed decreased staining for apoB and increased smooth muscle alpha-actin content, whereas accumulation of CD68-positive inflammatory cells was unchanged. We conclude that the perlecan HS chains are proatherogenic in mice, possibly through increased lipoprotein retention, altered vascular permeability, or other mechanisms. The ability of HS to inhibit smooth muscle cell growth may also influence development as well as instability of lesions.
authors.affiliation: Department of Molecular Medicine and Surgery, Karolinska University Hospital, Karolinska Institutet, SE-17176 Stockholm, Sweden. karin.tran.lundmark@ki.se
authors.forename: Karin
authors.initials: K
authors.lastname: Tran-Lundmark
authors.forename: Phan-Kiet
authors.initials: PK
authors.lastname: Tran
authors.forename: Gabrielle
authors.initials: G
authors.lastname: Paulsson-Berne
authors.forename: Vincent
authors.initials: V
authors.lastname: Fridén
authors.forename: Raija
authors.initials: R
authors.lastname: Soininen
authors.forename: Karl
authors.initials: K
authors.lastname: Tryggvason
authors.forename: Thomas N
authors.initials: TN
authors.lastname: Wight
authors.forename: Michael G
authors.initials: MG
authors.lastname: Kinsella
authors.forename: Jan
authors.initials: J
authors.lastname: Borén
authors.forename: Ulf
authors.initials: U
authors.lastname: Hedin
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism; D001011:Aorta / Q000378:metabolism / Q000473:pathology; D001055:Apolipoproteins B / Q000378:metabolism; D001057:Apolipoproteins E / Q000235:genetics / Q000378:metabolism*; D050197:Atherosclerosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002199:Capillary Permeability / Q000235:genetics; D049109:Cell Proliferation; D003433:Crosses, Genetic; D004195:Disease Models, Animal; D019812:Heparan Sulfate Proteoglycans / Q000235:genetics / Q000378:metabolism*; D006497:Heparan Sulfate / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D008077:Lipoproteins, LDL / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology; D011485:Protein Binding / Q000235:genetics; D014280:Triglycerides / Q000378:metabolism*; D000097382:CD68 Molecule
chemical_list: D000199:Actins; D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D001055:Apolipoproteins B; D001057:Apolipoproteins E; D019812:Heparan Sulfate Proteoglycans; D006497:Heparan Sulfate; D008077:Lipoproteins, LDL; D014280:Triglycerides; C081026:CD68 protein, mouse; D000097382:CD68 Molecule; C071927:perlecan