title: Kinetics of alpha-amylase synthesis from Bacillus amyloliquefaciens.
abstract: The microbial production of alpha-amylase from Bacillus amyloliquefaciens was investigated. The microorganism was grown using media containing glucose or maltose at 37 degrees C and under aerobic conditions in a 16-L fermentor. The alpha-amylase synthesis from maltose was not found to be inducible but was found to be subject to catabolite repression. The maltose uptake rate was observed to be the rate-limiting step compared to the conversion rate of maltose to glucose by intracellular alpha-glucosidase. The alpha-amylase activity achieved with maltose as a substrate was higher than that achieved with glucose. A slower growth rate and a higher cell density were obtained with maltose. The enzyme production pattern depended upon the nutrient composition of the medium.
authors.affiliation: Department of Chemical and Nuclear Engineering, University of Maryland, College Park, Maryland 20742.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Yoo
authors.forename: T W
authors.initials: TW
authors.lastname: Cadman
authors.forename: J
authors.initials: J
authors.lastname: Hong
authors.forename: R T
authors.initials: RT
authors.lastname: Hatch