Spermidine exodus and oxidation in the apoplast induced by abiotic stress is responsible for H2O2 signatures that direct tolerance responses in tobacco.
Polyamines (PAs) exert a protective effect against stress challenges, but their molecular role in this remains speculative. In order to detect the signaling role of apoplastic PA-derived hydrogen peroxide (H2O2) under abiotic stress, we developed a series of tobacco (Nicotiana tabacum cv Xanthi) transgenic plants overexpressing or downregulating apoplastic polyamine oxidase (PAO; S-pao and A-pao plants, respectively) or downregulating S-adenosyl-l-methionine decarboxylase (samdc plants). Upon salt stress, plants secreted spermidine (Spd) into the apoplast, where it was oxidized by the apoplastic PAO, generating H2O2. A-pao plants accumulated less H2O2 and exhibited less programmed cell death (PCD) than did wild-type plants, in contrast with S-pao and samdc downregulating plants. Induction of either stress-responsive genes or PCD was dependent on the level of Spd-derived apoplastic H2O2. Thus, in wild-type and A-pao plants, stress-responsive genes were efficiently induced, although in the latter at a lower rate, while S-pao plants, with higher H2O2 levels, failed to accumulate stress-responsive mRNAs, inducing PCD instead. Furthermore, decreasing intracellular PAs, while keeping normal apoplastic Spd oxidation, as in samdc downregulating transgenic plants, caused enhanced salinity-induced PCD. These results reveal that salinity induces the exodus of Spd into the apoplast, where it is catabolized by PAO, producing H2O2. The accumulated H2O2 results in the induction of either tolerance responses or PCD, depending also on the levels of intracellular PAs.
Moschou
Panagiotis N
PN
Department of Biology, University of Crete, 71409 Heraklion, Greece.
Paschalidis
Konstantinos A
KA
Delis
Ioannis D
ID
Andriopoulou
Athina H
AH
Lagiotis
George D
GD
Yakoumakis
Dimitrios I
DI
Roubelakis-Angelakis
Kalliopi A
KA
D000222:Adaptation, Physiological / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D012437:Adenosylmethionine Decarboxylase / Q000235:genetics / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D018525:Germination / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism*; D046529:Microscopy, Electron, Transmission; D010084:Oxidation-Reduction / Q000187:drug effects; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D017382:Reactive Oxygen Species / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012639:Seeds / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D012965:Sodium Chloride / Q000494:pharmacology; D013095:Spermidine / Q000378:metabolism* / Q000494:pharmacology; D014026:Nicotiana / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D000097667:Polyamine Oxidase
D012437:Adenosylmethionine Decarboxylase; D006861:Hydrogen Peroxide; D000587:Oxidoreductases Acting on CH-NH Group Donors; D017382:Reactive Oxygen Species; D012965:Sodium Chloride; D013095:Spermidine; D000097667:Polyamine Oxidase