title: Echinococcus granulosus: induction of T-independent antibody response against protoscolex glycoconjugates in early experimental infection.
abstract: In this work CD4-knockout mice were used as a model to analyse the role of CD4+ T cells in the antibody response against Echinococcus granulosus immunization or experimental infection. Results obtained with mice immunized with protoscolex antigens indicated that these contain T-independent antigens. After infection, CD4-knockout mice and C57Bl/6 mice showed similar titres of specific antibodies indicating that T-independent antibody production was quantitatively important in early infection. We have also identified an antigenic fraction from protoscoleces (E4+) which induces CD4 T cell independent antibody response in early stages of infection. In conclusion, the results presented here directly support the existence of T-independent immunogens in E. granulosus protoscoleces and suggest that T-independent antibody response may be quantitatively important in early infection.
authors.affiliation: Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
authors.forename: Adriana
authors.initials: A
authors.lastname: Baz
authors.affiliation: Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
authors.forename: Hernán
authors.initials: H
authors.lastname: Carol
authors.affiliation: Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
authors.forename: Verónica
authors.initials: V
authors.lastname: Fernández
authors.affiliation: Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Mourglia-Ettlin
authors.affiliation: Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
authors.forename: Alberto
authors.initials: A
authors.lastname: Nieto
authors.affiliation: Immunology Laboratory, Microbiology and Tumor Biology Center, Karolinska Institute, Stockholm, Sweden.
authors.forename: Anders
authors.initials: A
authors.lastname: Örn
authors.affiliation: Cátedra de Inmunología, Dpto. Biociencias, Facultad de Química, Universidad de la República, Casilla de Correos 1157, Montevideo, Uruguay.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Dematteis
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis* / Q000097:blood; D000947:Antigens, Helminth / Q000276:immunology*; D015704:CD4 Antigens / Q000235:genetics; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D004443:Echinococcosis / Q000276:immunology*; D048209:Echinococcus granulosus / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D000939:Epitopes / Q000276:immunology; D005260:Female; D006001:Glycoconjugates / Q000276:immunology; D007114:Immunization / Q000379:methods; D007074:Immunoglobulin G / Q000096:biosynthesis* / Q000097:blood / Q000145:classification; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout
chemical_list: D000909:Antibodies, Helminth; D000947:Antigens, Helminth; D015704:CD4 Antigens; D000939:Epitopes; D006001:Glycoconjugates; D007074:Immunoglobulin G