Apoplastic polyamine oxidation plays different roles in local responses of tobacco to infection by the necrotrophic fungus Sclerotinia sclerotiorum and the biotrophic bacterium Pseudomonas viridiflava.
The role of polyamine (PA) metabolism in tobacco (Nicotiana tabacum) defense against pathogens with contrasting pathogenic strategies was evaluated. Infection by the necrotrophic fungus Sclerotinia sclerotiorum resulted in increased arginine decarboxylase expression and activity in host tissues, as well as putrescine and spermine accumulation in leaf apoplast. Enhancement of leaf PA levels, either by using transgenic plants or infiltration with exogenous PAs, led to increased necrosis due to infection by S. sclerotiorum. Specific inhibition of diamine and PA oxidases attenuated the PA-induced enhancement of leaf necrosis during fungal infection. When tobacco responses to infection by the biotrophic bacterium Pseudomonas viridiflava were investigated, an increase of apoplastic spermine levels was detected. Enhancement of host PA levels by the above-described experimental approaches strongly decreased in planta bacterial growth, an effect that was blocked by a PA oxidase inhibitor. It can be concluded that accumulation and further oxidation of free PAs in the leaf apoplast of tobacco plants occurs in a similar, although not identical way during tobacco defense against infection by microorganisms with contrasting pathogenesis strategies. This response affects the pathogen's ability to colonize host tissues and results are detrimental for plant defense against necrotrophic pathogens that feed on necrotic tissue; on the contrary, this response plays a beneficial role in defense against biotrophic pathogens that depend on living tissue for successful host colonization. Thus, apoplastic PAs play important roles in plant-pathogen interactions, and modulation of host PA levels, particularly in the leaf apoplast, may lead to significant changes in host susceptibility to different kinds of pathogens.
Marina
María
M
Universidad Nacional de General San Martín-Consejo Nacional de Investigaciones Científicas y Técnicas, B7130IWA Chascomus, Argentina.
Maiale
Santiago Javier
SJ
Rossi
Franco Rubén
FR
Romero
Matías Fernando
MF
Rivas
Elisa Isabel
EI
Gárriz
Andrés
A
Ruiz
Oscar Adolfo
OA
Pieckenstain
Fernando Luis
FL
D006631:Amine Oxidase (Copper-Containing) / Q000037:antagonists & inhibitors; D001203:Ascomycota / Q000502:physiology*; D002262:Carboxy-Lyases / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D009336:Necrosis / Q000382:microbiology; D010084:Oxidation-Reduction; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000378:metabolism*; D011073:Polyamines / Q000378:metabolism*; D011549:Pseudomonas / Q000502:physiology*; D011700:Putrescine / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D013096:Spermine / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology / Q000378:metabolism / Q000382:microbiology*; D000097667:Polyamine Oxidase
D006631:Amine Oxidase (Copper-Containing); D002262:Carboxy-Lyases; D000587:Oxidoreductases Acting on CH-NH Group Donors; D010936:Plant Extracts; D010940:Plant Proteins; D011073:Polyamines; D011700:Putrescine; D012333:RNA, Messenger; D017382:Reactive Oxygen Species; D013096:Spermine; D000097667:Polyamine Oxidase; C019722:arginine decarboxylase