[Indication and identification of Lactobacillus spp. with the use of polymerase chain reaction].
OBJECTIVE
The aim of the work is the development of laboratory test for indication and identification of Lactobacillus spp. by the polymerase chain reaction.


METHODS
The work is developed on the base of the GenBank/EMBL data about genetic sequences of the Lactobacillus spp. The sequences of DNA were studied with the help of the ClustalW program. The strains of the Lactobacillus spp., which are the object of the research, have been registered in Russian collection of industrial microorganisms.


RESULTS
The laboratory test of nested-PCR for indication and identification L. plantarum, L. fermentum, L. acidophilus, L. delbrueckii, L. casei, L. rhamnosus was performed. The specificity of the nested-PCR was correlated with the control analyses of monoculture Lactobacillus spp. and commercial products.


CONCLUSIONS
The new developed laboratory nested-PCR test may be use in control system of milk foods enriched by probiotic microorganisms.
Tochilina
A G
AG
Novikova
N A
NA
Sokolova
K Ia
KIa
Solov'eva
I V
IV
Belova
I V
IV
Ivanova
T P
TP
Lukovnikova
L B
LB
Golotsyna
L N
LN
D015373:Bacterial Typing Techniques / Q000379:methods; D017931:DNA Primers; D007778:Lactobacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods*; D019936:Probiotics / Q000145:classification* / Q000302:isolation & purification*; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012680:Sensitivity and Specificity; D012984:Software
D017931:DNA Primers; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S