PCR detection of type I polyketide synthase genes in myxobacteria.
The diversity of type I modular polyketide synthase (PKS) was explored by PCR amplification of DNA encoding ketosynthase and acyltransferase domains in myxobacteria. The sequencing of the amplicons revealed that many PKS genes were distantly related to the published sequences. Thus, myxobacteria may be excellent resources for novel and diverse polyketides.
Komaki
Hisayuki
H
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan. hskomaki@nbrc.nite.go.jp
Fudou
Ryosuke
R
Iizuka
Takashi
T
Nakajima
Daisuke
D
Okazaki
Koei
K
Shibata
Daisuke
D
Ojika
Makoto
M
Harayama
Shigeaki
S
D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009231:Myxococcales / Q000201:enzymology / Q000235:genetics*; D010802:Phylogeny; D048630:Polyketide Synthases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
D004269:DNA, Bacterial; D048630:Polyketide Synthases