title: Novel recombinant congenic mouse strain developing arthritis with enthesopathy.
abstract: Based on the hypothesis that the complex pathological and immunological manifestations of rheumatoid arthritis (RA) and the related diseases are under the control of multiple gene loci with allelic polymorphism, a recombinant congenic mouse strain was prepared between an MRL/Mp-lpr/lpr (MRL/lpr) strain, which develops arthritis resembling RA, and a non-arthritic strain C3H/HeJ-lpr/lpr (C3H/lpr). In MRL/lpr x (MRL/lpr x C3H/lpr) F1 mice, the mice developing severe arthritis were selected based on joint swelling to further continue intercrosses, and then an McH-lpr/lpr-RA1 (McH/lpr-RA1) strain was established and its histopathological phenotypes of joints and autoimmune traits were analyzed. Arthritis in McH/lpr-RA1 mice developed at a higher incidence by 20 weeks of age, compared with that in the MRL/lpr mice, who had severe synovitis (ankle, 60.3%; knee, 65.1%), and also fibrous and fibrocartilaginous lesions of articular ligamenta resembling enthesopathy (ankle, 79.4%; knee, 38.1%), resulting in ankylosis. The lymphoproliferative disorder was less, and serum levels of IgG and IgG autoantibodies including anti-dsDNA and rheumatoid factor were lower than those of both MRL/lpr and C3H/lpr strains. McH/lpr-RA1 mice may provide a new insight into the study of RA regarding the common genomic spectrum of seronegative RA and enthesopathy.
authors.affiliation: Tohoku University Graduate School of Dentistry, Sendai, Japan.
authors.forename: Shiro
authors.initials: S
authors.lastname: Mori
authors.forename: Naoko
authors.initials: N
authors.lastname: Tanda
authors.forename: Mitsuko R
authors.initials: MR
authors.lastname: Ito
authors.forename: Hisashi
authors.initials: H
authors.lastname: Oishi
authors.forename: Takahito
authors.initials: T
authors.lastname: Tsubaki
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Komori
authors.forename: Ming-Cai
authors.initials: MC
authors.lastname: Zhang
authors.forename: Masao
authors.initials: M
authors.lastname: Ono
authors.forename: Masahiko
authors.initials: M
authors.lastname: Nishimura
authors.forename: Masato
authors.initials: M
authors.lastname: Nose
mesh_terms: D000818:Animals; D000843:Ankle Joint / Q000473:pathology; D000844:Ankylosis / Q000453:epidemiology; D001172:Arthritis, Rheumatoid / Q000235:genetics* / Q000276:immunology* / Q000473:pathology; D001323:Autoantibodies / Q000097:blood; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry; D007136:Immunoglobulins / Q000097:blood; D007719:Knee Joint / Q000473:pathology; D008214:Lymphocytes / Q000166:cytology / Q000276:immunology; D008297:Male; D051379:Mice; D020297:Mice, Congenic / Q000235:genetics / Q000276:immunology; D019463:Mice, Inbred MRL lpr; D013585:Synovitis; D014657:Vasculitis / Q000453:epidemiology
chemical_list: D001323:Autoantibodies; D007136:Immunoglobulins