Genome sequence of the lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078: evolutionary relationships to phages from Corynebacterineae.
P1201 is a lytic corynephage of Corynebacterium glutamicum NCHU 87078. Its genome consists of a linear double-stranded DNA molecule of 70,579 base pairs, with 3'-protruding cohesive ends of ten nucleotides. We have identified 69 putative open reading frames, including three apparent genes (thymidylate synthase, terminase, and RNR alpha subunit genes) that are interrupted by an intein. Protein-splicing activities of these inteins were demonstrated in Escherichia coli. Three structural proteins including major capsid and major tail proteins were separated by SDS-PAGE and identified by both LC-MS-MS and N-terminal sequence analyses. Bioinformatics analysis indicated that only about 8.7% of its putative gene products shared substantial protein sequence similarity with the lytic corynephage BFK20 from Brevibacterium flavum, the only corynephage whose genome had been sequenced to date, revealing that the P1201 genome is distinct from BFK20. The mosaic-like genome of P1201 indicates extensive horizontal gene transfer among P1201, Gordonia terrae phage GTE5, mycobacteriophages, and several regions of Corynebacterium spp. genomes.
Chen
Chang-Lin
CL
Institute of Molecular Biology, National Chung Hsing University, Taichung 402, Taiwan.
Pan
Tzu-Ying
TY
Kan
Shu-Chen
SC
Kuan
Yi-Chia
YC
Hong
Lian-Yu
LY
Chiu
Kun-Ruei
KR
Sheu
Ching-Sen
CS
Yang
Jui-Sen
JS
Hsu
Wen-Hwei
WH
Hu
Hui-Yu
HY
D001435:Bacteriophages / Q000235:genetics* / Q000648:ultrastructure; D002853:Chromatography, Liquid; D048230:Corynebacterium glutamicum / Q000821:virology*; D004247:DNA / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry* / Q000235:genetics*; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000235:genetics; D023061:Gene Order; D022761:Gene Transfer, Horizontal; D016679:Genome, Viral; D047668:Inteins; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D019154:Protein Splicing; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D053719:Tandem Mass Spectrometry; D014764:Viral Proteins / Q000235:genetics / Q000302:isolation & purification; D014771:Virion / Q000648:ultrastructure
D004269:DNA, Bacterial; D014764:Viral Proteins; D004247:DNA