Intracellular localization of the heat shock protein, HSP110, in Xenopus laevis A6 kidney epithelial cells.
Heat shock protein 110 (HSP110) is a large molecular mass chaperone that is part of the HSP70/DnaK superfamily. In the present study, we examined the accumulation of HSP110 in Xenopus laevis A6 kidney epithelial cells. Immunoblot analysis, using a homologous antibody, detected the presence of HSP110 in A6 cells maintained at 22 degrees C. The relative levels of HSP110 accumulation increased after heat shock or sodium arsenite treatment. Immunocytochemical analysis revealed that constitutively expressed HSP110 was localized in the cytoplasm in a diffuse granular pattern with enrichment in the nucleus. In A6 cells heat shocked at 33 degrees C or 35 degrees C for 2 to 4 h, HSP110 accumulation was enhanced and detected primarily in the cytoplasm as thread- or spindle-like structures. In contrast, HSP30 was not detected constitutively and heat shock treatment of A6 cells induced a relatively uniform punctate pattern primarily in the cytoplasm. Also, treatment of A6 cells at 35 degrees C for 6 h resulted in the presence of HSP110 and HSP30 enriched in the nucleus of most cells. Finally, A6 cells treated with 25 microM sodium arsenite produced very dense HSP110 structures primarily in the cytoplasm while HSP30 was enriched in the cytoplasm in a granular pattern.
Gauley
J
J
Department of Biology, University of Waterloo, Waterloo, ON N2L3G1, Canada.
Young
J T F
JT
Heikkila
J J
JJ
D000818:Animals; D018053:Arsenites / Q000494:pharmacology; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D050957:HSP110 Heat-Shock Proteins / Q000378:metabolism*; D050890:HSP30 Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response / Q000187:drug effects; D042541:Intracellular Space / Q000187:drug effects / Q000378:metabolism*; D007668:Kidney / Q000166:cytology*; D021381:Protein Transport / Q000187:drug effects; D017670:Sodium Compounds / Q000494:pharmacology; D013696:Temperature; D029867:Xenopus Proteins / Q000378:metabolism; D014982:Xenopus laevis / Q000378:metabolism*
D018053:Arsenites; D050957:HSP110 Heat-Shock Proteins; D050890:HSP30 Heat-Shock Proteins; D017670:Sodium Compounds; D029867:Xenopus Proteins; C494391:hsp30 protein, Xenopus; C017947:sodium arsenite