The response of human colonocytes to folate deficiency in vitro: functional and proteomic analyses.
Low folate intake is associated with colon cancer. We combined a proteomics and biochemical approach to identify proteins and pathways affected by folate deficiency in human colonocytes. Folate differentially altered activity and expression of proteins involved in proliferation [e.g., PCNA], DNA repair [e.g., XRCC5, MSH2], apoptosis [e.g., BAG family chaperone protein, DIABLO and porin], cytoskeletal organization [e.g., actin, ezrin, elfin], and expression of proteins implicated in malignant transformation [COMT, Nit2].
Duthie
Susan J
SJ
Rowett Research Institute, Bucksburn, Aberdeen, UK. sd@rri.sari.ac.uk
Mavrommatis
Yiannis
Y
Rucklidge
Gary
G
Reid
Martin
M
Duncan
Gary
G
Moyer
Mary P
MP
Pirie
Lynn P
LP
Bestwick
Charles S
CS
D000368:Aged; D017209:Apoptosis / Q000502:physiology; D015415:Biomarkers / Q000378:metabolism; D002450:Cell Communication / Q000502:physiology; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D003106:Colon / Q000166:cytology*; D003599:Cytoskeleton / Q000502:physiology; D004249:DNA Damage / Q000502:physiology; D004260:DNA Repair / Q000502:physiology; D004847:Epithelial Cells / Q000378:metabolism*; D005494:Folic Acid Deficiency / Q000378:metabolism*; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D008297:Male; D018384:Oxidative Stress / Q000502:physiology; D020543:Proteome / Q000378:metabolism*
D015415:Biomarkers; D020543:Proteome