DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans.
DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing alpha-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.
Wang
Sheng-Fan
SF
Department of Biotechnology and Laboratory Science in Medicine, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan.
Huang
Jason C
JC
Lee
Yuan-Ming
YM
Liu
Shih-Jen
SJ
Chan
Yu-Jiun
YJ
Chau
Yat-Pang
YP
Chong
Pele
P
Chen
Yi-Ming Arthur
YM
D000818:Animals; D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules / Q000276:immunology*; D002460:Cell Line; D003713:Dendritic Cells / Q000276:immunology / Q000821:virology; D004285:Dogs; D006387:Hemagglutination, Viral / Q000276:immunology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000737:chemistry / Q000276:immunology; D006801:Humans; D053124:Influenza A Virus, H5N1 Subtype / Q000235:genetics / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology* / Q000635:transmission; D007251:Influenza, Human / Q000276:immunology* / Q000635:transmission; D037181:Lectins, C-Type / Q000276:immunology*; D009439:Neuraminidase / Q000276:immunology; D011200:Poultry / Q000276:immunology / Q000821:virology; D011487:Protein Conformation; D011956:Receptors, Cell Surface / Q000276:immunology*; D014764:Viral Proteins / Q000276:immunology; D014771:Virion / Q000235:genetics / Q000276:immunology; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D000911:Antibodies, Monoclonal; D015815:Cell Adhesion Molecules; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D037181:Lectins, C-Type; D009439:Neuraminidase; D011956:Receptors, Cell Surface; D014764:Viral Proteins; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C109963:hemagglutinin, avian influenza A virus; C092488:sialic acid receptor; C487630:NA protein, influenza A virus