[Determination of vetatramine in Veratrum nigrum by HPLC-ELSD].
OBJECTIVE
To develop an HPLC-ELSD method for determination of vetatramine. in Veratrum nigrum.


METHODS
The analy fical column was Shim-pack ODS - C18 (4.6 mm x 250 mm, 4 microm) column, the mobile phase was acetonitrile-water (containing 0.1% triethylamine) (50:50), at a flow rate of 0.8 mL x min(-1). The temperature of drift tube was 90 degrees C and the gas flow was at the rate of 2.5 L x min(-1).


RESULTS
The calibration curve was linear in the range of 0.36-3.6 microg (r = 0.999 8). The average recovery was 100.9% (RSD 2.3%, n = 6). The contents of veratramine in Veratrum nigrum. from the ten different sources were determined.


CONCLUSIONS
The method may be used as a accurate and reproducible way to determine the content of veratramine in V. nigrum.
Wang
Li-Shu
LS
College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China. wls6856@163.com
Zhao
Da-Qing
DQ
Tao
Hua-Ming
HM
Liu
Yong-Hong
YH
D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D016014:Linear Models; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014703:Veratrum / Q000737:chemistry*; D014704:Veratrum Alkaloids / Q000032:analysis* / Q000302:isolation & purification
D004365:Drugs, Chinese Herbal; D014704:Veratrum Alkaloids; C009649:veratramine