title: [Effects of visfatin gene overexpression on insulin sensitivity in the insulin-resistant rats induced by high-fat diet].
abstract: OBJECTIVE
  To investigate the effects of visfatin gene overexpression on insulin sensitivity in insulin-resistant (IR) rats induced by high-fat diet.
  
  
  METHODS
  The recombinant visfatin plasmid was constructed and transfected into IR rats induced by high-fat diet. The euglycemic-hyperinsulinemic clamp experiments were performed for evaluation the change of insulin sensitivity before and after administration.
  
  
  RESULTS
  The expression plasmid of visfatin were successfully constructed. After 3 days for plasmid injecting, plasma visfatin levels and glucose infusion rates were significantly increased (2.19 +/- 0.36 vs 0.98 +/- 0.27 and 32.6 +/- 1.2 vs 24.0 +/- 1.2 mg x kg(-1) x min(-1), respectively, all P < 0.01), and total cholesterol and high-density lipoprotein cholesterol (HDL-C) were significantly decreased (2.36 +/- 0.22 vs 1.60 +/- 0.21 mmol/L and 1.41 +/- 0.24 vs 0.88 +/- 0.11 mmol/L, respectively, all P < 0.05) in high-fat diet rats.
  
  
  CONCLUSIONS
  The transfection of visfatin plasmid enhanced plasma visfatin level and improved insulin sensitivity in IR rats induced by high-fat diet.
authors.affiliation: Department of Endocrinology, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Qin
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.forename: Gang-Yi
authors.initials: GY
authors.lastname: Yang
authors.forename: Ren-Zhe
authors.initials: RZ
authors.lastname: Li
authors.forename: Ke
authors.initials: K
authors.lastname: Li
authors.forename: Xiao-Ya
authors.initials: XY
authors.lastname: Qi
authors.forename: Sheng-Bing
authors.initials: SB
authors.lastname: Li
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Chen
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
authors.forename: Guenther
authors.initials: G
authors.lastname: Boden
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western; D004041:Dietary Fats / Q000633:toxicity; D004351:Drug Resistance / Q000187:drug effects; D015870:Gene Expression / Q000187:drug effects; D015309:Glucose Clamp Technique; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000494:pharmacology; D007328:Insulin / Q000494:pharmacology*; D007333:Insulin Resistance; D008297:Male; D054409:Nicotinamide Phosphoribosyltransferase / Q000097:blood* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D001786:Blood Glucose; D004041:Dietary Fats; D007004:Hypoglycemic Agents; D007328:Insulin; D054409:Nicotinamide Phosphoribosyltransferase