title: Growth of tick-borne encephalitis virus (European subtype) in cell lines from vector and non-vector ticks.
abstract: We undertook a comparative study of the susceptibility of different tick cell lines to infection with the European subtype of tick-borne encephalitis virus (TBEV), prototype strain Neudoerfl. The growth of TBEV was investigated in lines derived from vector Ixodes ricinus L. ticks (IRE/CTVM18, 19, and 20), as well as non-vector ticks, namely Ixodes scapularis Say (IDE2), Boophilus microplus Canestrini (BME/CTVM2), Hyalomma anatolicum anatolicum Koch (HAE/CTVM9), Rhipicephalus appendiculatus Neumann (RA-257) and recently established and herein described lines from the argasid tick Ornithodoros moubata Murray (OME/CTVM21 and 22). All the tick cell lines tested were susceptible to infection by TBEV and the virus caused productive infection without any cytopathic effect. However, there was a clear difference between the TBEV growth in vector and non-vector cell lines, since I. ricinus cell lines produced 100-1000-fold higher virus yield than the non-vector cell lines. The lowest virus production was observed in O. moubata and R. appendiculatus cell lines.
authors.affiliation: Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic & Faculty of Science, University of South Bohemia, Ceské Budejovice, Czech Republic. ruzekd@paru.cas.cz
authors.forename: Daniel
authors.initials: D
authors.lastname: Růzek
authors.forename: Lesley
authors.initials: L
authors.lastname: Bell-Sakyi
authors.forename: Jan
authors.initials: J
authors.lastname: Kopecký
authors.forename: Libor
authors.initials: L
authors.lastname: Grubhoffer
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000378:metabolism; D001096:Arachnid Vectors / Q000166:cytology / Q000821:virology*; D002460:Cell Line; D004669:Encephalitis Viruses, Tick-Borne / Q000254:growth & development*; D013987:Ticks / Q000166:cytology / Q000821:virology*; D013997:Time Factors; D014776:Virus Cultivation; D014779:Virus Replication / Q000502:physiology*
chemical_list: D000914:Antibodies, Viral