title: Whole blood coagulation assessment using rotation thrombelastogram thromboelastometry in patients with acute deep vein thrombosis.
abstract: Common tests for the assessment of blood coagulation in the acute phase of deep vein thrombosis are of limited value for the evaluation of the associated hypercoagulability. The new rotation thromboelastometry by rotation thrombelastogram has the potential to provide information on whole blood clot formation and prothrombotic state in patients with acute deep vein thrombosis. Rotation thrombelastogram parameters were evaluated in whole blood of 30 patients with a first episode of acute deep vein thrombosis and 40 healthy controls. The effect of factor VIII and fibrinogen levels on rotation thrombelastogram assays was also assessed in the study population and in a model of blood supplemented by increasing amounts of fibrinogen. All assays performed were consistent with a remarkable hypercoagulable profile in deep vein thrombosis patients as compared with controls. In particular, maximum clot firmness and the area under curve values, which are expected to better correlate with the hypercoagulable state in the acute phase of deep vein thrombosis, were significantly higher in patients than in controls. As expected, fibrinogen was shown to be one of the main determinants of the hypercoagulability in rotation thrombelastogram assays. In a small subset of acute deep vein thrombosis patients, inherited thrombophilia had no influence on rotation thrombelastogram parameters. The new rotation thrombelastogram thromboelastometry is a useful tool to detect acute deep vein thrombosis-related hypercoagulability. Prospective studies are needed to define the potential applications of rotation thrombelastogram in the management of deep vein thrombosis patients.
authors.affiliation: Department of Medical and Surgical Sciences, 2nd Chair of Internal Medicine, University of Padua, Medical School, Padua, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Spiezia
authors.forename: Paolo
authors.initials: P
authors.lastname: Marchioro
authors.forename: Claudia
authors.initials: C
authors.lastname: Radu
authors.forename: Valeria
authors.initials: V
authors.lastname: Rossetto
authors.forename: Giulio
authors.initials: G
authors.lastname: Tognin
authors.forename: Castelli
authors.initials: C
authors.lastname: Monica
authors.forename: Laura
authors.initials: L
authors.lastname: Salmaso
authors.forename: Paolo
authors.initials: P
authors.lastname: Simioni
mesh_terms: D000208:Acute Disease; D000328:Adult; D000368:Aged; D001777:Blood Coagulation; D005169:Factor VIII / Q000032:analysis; D005260:Female; D005340:Fibrinogen / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D008991:Monitoring, Physiologic / Q000379:methods*; D013916:Thrombelastography / Q000379:methods*; D020246:Venous Thrombosis / Q000097:blood*
chemical_list: D005169:Factor VIII; D005340:Fibrinogen