Generation of rat monoclonal antibodies against human RANTES.
RANTES (or regulated upon activation, normal T cell expressed and secreted) belongs to the rapidly growing chemokine family. It is mainly produced by T cells, epithelial cells, monocytes, fibroblasts, and mesanglial cells. Increased RANTES expression has been associated with a wide range of inflammatory disorders and pathologies. Mouse RANTES is the homolog molecule of human RANTES. The two have considerable homology in both sequence and structure. Using hRANTES as immunogen and the technique of rat B lymphocyte hybridoma, we raised two hybridoma cell lines secreting monoclonal antibodies (MAbs) to hRANTES, designated no. 1 and no. 2. Both MAbs can bind the hRANTES in FCM, Western blot analysis, and immunocytochemistry. No. 1 also worked well in immunohistochemistry of rat transplanted intestine, which may recognize the same epitope on human RANTES and rat RANTES. Thus, successful production of rat anti-human RANTES MAbs may provide a useful tool in further exploration of the biological function and pathological significance of RANTES and may provide a new method to judge early rejection after small bowel transplantation.
Kang
Zhen-Hua
ZH
Department of Gastrointestinal Surgery, Xi Jing Hospital, Fourth Military Medical University, Xi'an, China.
Li
Meng-Bin
MB
Wang
Chun-Yan
CY
Dong
Guang-Long
GL
Zhang
Hong-Wei
HW
Yang
Jian-Jun
JJ
Zheng
Jian-Yong
JY
Li
Ji-Peng
JP
Wang
Wei-Zhong
WZ
D000818:Animals; D000906:Antibodies / Q000737:chemistry; D000911:Antibodies, Monoclonal / Q000737:chemistry*; D002460:Cell Line; D045744:Cell Line, Tumor; D018946:Chemokine CCL5 / Q000737:chemistry*; D006801:Humans; D006825:Hybridomas / Q000378:metabolism; D007150:Immunohistochemistry / Q000379:methods; D007155:Immunologic Factors / Q000737:chemistry; D007413:Intestinal Mucosa / Q000378:metabolism; D019169:Jurkat Cells; D051379:Mice; D016377:Organ Transplantation / Q000379:methods; D051381:Rats
D000906:Antibodies; D000911:Antibodies, Monoclonal; D018946:Chemokine CCL5; D007155:Immunologic Factors