title: [Prediction and analysis of the secreteomic in Trichoderma reesei].
abstract: OBJECTIVE
  We studied the secretomics' properties of Trichoderma reesei, an important industrial microorganism used for cellulase production.
  
  
  METHODS
  We analyzed the amino acid sequences coded by 9997 ORFs in Trichoderma reesei genome with bioinformatics approaches, identified 294 possible secreted protein sequences, and classified them by functions. We also applied motif search methods to search key motifs in the function-unknown sequences and preliminary predicted their functions. Moreover, we analyzed the signal peptide sequences of the secreted proteins.
  
  
  RESULTS
  There were 188 hydrolytic enzymes in Trichoderma reesei's secretomics, including 114 glycosidases, 42 proteases, and 11 lipoidases. The glycosidases included 22 reported cellulases, and 15 chitinases, as well as 30 other protein sequences probably related to cellulose degradation. The homology of signal peptides of secreted proteins was low whereas sequences near the digesting site of signalase were conservative.
  
  
  CONCLUSIONS
  This method gave insights into the whole secreted proteome of Trichoderma reesei and provided basis for further studies on secretomic features at a genome level.
authors.affiliation: College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 210009, China. jiongjiong821@163.com
authors.forename: Wen
authors.initials: W
authors.lastname: Tang
authors.forename: Ming
authors.initials: M
authors.lastname: Yan
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D001665:Binding Sites; D002480:Cellulase / Q000096:biosynthesis; D005656:Fungal Proteins / Q000032:analysis* / Q000235:genetics / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010447:Peptide Hydrolases / Q000235:genetics / Q000378:metabolism; D021382:Protein Sorting Signals; D014242:Trichoderma / Q000145:classification / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D021382:Protein Sorting Signals; D006026:Glycoside Hydrolases; D002480:Cellulase; D010447:Peptide Hydrolases