Toward improved biochips based on rolling circle amplification--influences of the microenvironment on the fluorescence properties of labeled DNA oligonucleotides.
Microarrays have become an increasingly important tool for biotechnology and molecular diagnostics. Despite many advantages, their sensitivity is still insufficient for such tasks as the analysis of small sample quantities and for the detection of alterations in gene expression of low-abundance genes. Accordingly, amplification strategies are necessary. Approaches to amplify the signal intensity include the increase of the number of dye molecules per target through either particle labels or rolling circle amplification, as used for this study.
Mayer-Enthart
Elke
E
Federal Institute for Materials Research and Testing (BAM), I.5 Bioanalytics, Richard-Willstätter-Str. 11, 12489 Berlin, Germany. elke.mayer-enthart@bam.de
Sialelli
Julien
J
Rurack
Knut
K
Resch-Genger
Ute
U
Köster
Daniela
D
Seitz
Harald
H
D002232:Carbocyanines / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D011088:DNA Ligases; D004270:DNA, Circular / Q000737:chemistry*; D004277:DNA, Single-Stranded; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000494:pharmacology*; D008956:Models, Chemical; D008957:Models, Genetic; D021141:Nucleic Acid Amplification Techniques; D009690:Nucleic Acid Conformation; D020411:Oligonucleotide Array Sequence Analysis; D015345:Oligonucleotide Probes / Q000737:chemistry; D009841:Oligonucleotides / Q000737:chemistry*
D002232:Carbocyanines; D004270:DNA, Circular; D004277:DNA, Single-Stranded; D005456:Fluorescent Dyes; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; C089187:cyanine dye 3; D004247:DNA; D011088:DNA Ligases