Improved pulsed field gel electrophoresis method for Moraxella catarrhalis.
An improved PFGE method for the molecular typing of Moraxella catarrhalis is described. A modified PulseNet method using higher concentrations of EDTA and proteinase K, together with increased reagent volumes and incubation temperatures resulted in improved results and a more rapid turnaround time compared to PFGE methods currently used.
Pingault
Nevada M
NM
School of Biomedical, Biomolecular and Chemical Sciences, The University of Western Australia, Perth, Western Australia, Australia. Nevada.Pingault@health.wa.gov.au
Lehmann
Deborah
D
Riley
Thomas V
TV
D015373:Bacterial Typing Techniques / Q000379:methods*; D004269:DNA, Bacterial / Q000032:analysis; D004492:Edetic Acid / Q000737:chemistry; D016521:Electrophoresis, Gel, Pulsed-Field / Q000379:methods*; D019286:Endopeptidase K / Q000378:metabolism; D006801:Humans; D001936:Moraxella catarrhalis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D045828:Moraxellaceae Infections / Q000382:microbiology
D004269:DNA, Bacterial; D004492:Edetic Acid; D019286:Endopeptidase K