Bone regeneration using titanium nonwoven fabrics combined with fgf-2 release from gelatin hydrogel microspheres in rabbit skull defects.
The objective of this study was to modify titanium nonwoven fabrics (Ti) with a hydroxyapatite (HA)-like coating and fibroblast growth factor (FGF)-2 combination, and evaluate the bone regeneration potential of the modified Ti. Biodegradable gelatin hydrogel microspheres (GM) were prepared as a carrier matrix for the controlled release of FGF-2. Ti, HA-coated Ti (Ti-HA), and Ti-HA incorporating GM (Ti-HA-GM) infused FGF-2 were applied to skull defects of rabbits. Then osteointegration in the Ti was evaluated by alkaline phosphatase activity, Ca(2+) content, and histological observation, and the hemoglobin content was assessed for angiogenic measurement. Ti-HA-GM promoted bone regeneration to a significantly greater extent than Ti, Ti-HA, or mixed Ti-HA and free FGF-2 6 weeks after application, and it also enhanced the hemoglobin content. It is concluded that the combination of HA-like coating and FGF-2 release promotes Ti induction of bone regeneration.
Ichinohe
Norihisa
N
Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan. yasuhiko@frontier.kyoto-u.ac.jp
Kuboki
Yoshinori
Y
Tabata
Yasuhiko
Y
D000818:Animals; D001861:Bone Regeneration / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry; D002118:Calcium / Q000378:metabolism; D016222:Fibroblast Growth Factor 2 / Q000737:chemistry* / Q000378:metabolism; D005780:Gelatin / Q000737:chemistry; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011817:Rabbits; D012886:Skull / Q000503:physiopathology / Q000601:surgery*; D014025:Titanium / Q000737:chemistry*
D018786:Bone Substitutes; D016222:Fibroblast Growth Factor 2; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D005780:Gelatin; D014025:Titanium; D002118:Calcium