[Roles of spermidine in induced resistance of cucumber seedlings to Botrytis cinerea Pers].
Under the conditions of root substrate injection and foliar spray of different concentration spermidine, this paper measured the disease index, defense enzyme activities, and contents of disease resistance-related substances in cucumber (Cucumis sativus L.) seedlings infected by Botrytis cinerea Pers., aimed to investigate the roles of spermidine in induced resistance of cucumber seedlings to B. cinerea. The results showed that after treated with spermidine, the disease index decreased by 25.4%, phenylalanine ammonia lyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) activities increased by 48.4%, 635.9% and 38.9%, respectively, and total polyphenol, flavonoid and lignin contents increased significantly, compared with the control. All of these suggested that spermidine could induce the disease resistance of cucumber seedlings, and its optimum concentration was 150 mg L(-1).
Shang
Qing-mao
QM
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China. shangqm@mail.caas.net.cn
Zhang
Zhi-gang
ZG
D020171:Botrytis / Q000187:drug effects*; D004156:Catechol Oxidase / Q000378:metabolism; D018553:Cucumis sativus / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D005419:Flavonoids / Q000378:metabolism; D007113:Immunity, Innate / Q000187:drug effects; D008031:Lignin / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010636:Phenols / Q000378:metabolism; D010650:Phenylalanine Ammonia-Lyase / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D059808:Polyphenols; D036226:Seedlings / Q000187:drug effects* / Q000378:metabolism / Q000382:microbiology; D013095:Spermidine / Q000494:pharmacology*
D005419:Flavonoids; D010636:Phenols; D059808:Polyphenols; D008031:Lignin; D004156:Catechol Oxidase; D009195:Peroxidase; D010650:Phenylalanine Ammonia-Lyase; D013095:Spermidine