title: Temperature dependence of near-infrared spectra of whole blood.
abstract: The temperature dependence (30 to 40 degrees C) of near-infrared spectra (500 to 1100 nm) of whole human blood, including red blood cells with intact physiological function, is investigated. Previous studies have focused on hemoglobin solutions, but the operation of red blood cells is critically dependent on intact cell membranes to perform normal oxygen transport and other physiological functions. Thus measurements of whole blood are more directly related to changes that occur in vivo. In addition to the response of hemoglobin to temperature in the spectra, a temperature response from water in the plasma is also detected. The temperature response of the water absorption at 960 nm is approximately ten times smaller than the temperature response of the oxyhemoglobin component in the blood at 610 nm. However, it is the most significant temperature effect between 800 and 1000 nm. This work will aid the precision and understanding of full spectrum near-infrared measurements on blood.
authors.affiliation: The Food and Horticulture Research Institute of New Zealand, Hamilton, New Zealand 3240. pmartinsen@hortresearch.co.nz
authors.forename: Paul
authors.initials: P
authors.lastname: Martinsen
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Charlier
authors.forename: Tim
authors.initials: T
authors.lastname: Willcox
authors.forename: Guy
authors.initials: G
authors.lastname: Warman
authors.forename: Andrew
authors.initials: A
authors.lastname: McGlone
authors.forename: Rainer
authors.initials: R
authors.lastname: Künnemeyer
mesh_terms: D001774:Blood Chemical Analysis; D001790:Blood Physiological Phenomena; D006801:Humans; D010108:Oxyhemoglobins / Q000737:chemistry*; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D013696:Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D010108:Oxyhemoglobins; D014867:Water