Sequential and quantitative analysis of a murine model of elastase-induced emphysema.
Emphysema, one of chronic obstructive pulmonary disease (COPD), is characterized as destruction of airway wall and small airway inflammation. To assess the kinetics of disease progression in murine model of elastase-induced emphysema, we used micro-computed tomography (CT) compared with morphological changes. Two week after elastase administration, a significant increase in the volume of low-density areas, recognized as -800--600 Hounsfield units by micro-CT, was observed. Coefficient of correlation between mean linear intercept (Lm) and low-density area examined by CT, was 0.79 (p<0.01). Micro-CT can quantitatively and sequentially detect murine emphysematous changes, offering a practical method to sequentially analyze the therapeutic effects of treatments in a murine model of emphysema.
Kawakami
Masaki
M
Department of Pharmatherapy, Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan.
Matsuo
Yukiko
Y
Yoshiura
Kenta
K
Nagase
Takahide
T
Yamashita
Naomi
N
D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004646:Emphysema / Q000139:chemically induced* / Q000473:pathology*; D007091:Image Processing, Computer-Assisted; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010196:Pancreatic Elastase; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014057:Tomography, X-Ray Computed
D012333:RNA, Messenger; D010196:Pancreatic Elastase