Roles of the first and second round of DNA replication in the regulation of zygotic gene activation in mice.
In the mouse embryo, expression of zygotic genes starts in the S/G2 phase of the 1-cell stage and greatly increases during the 2-cell stage. Although the timing of zygotic gene activation (ZGA) is thus established, the mechanism regulating ZGA is poorly understood. Previous studies using reporter genes have suggested that a transcriptionally repressive state is established during the 2-cell stage and that the first and second rounds of DNA replication are involved in this process. To further elucidate the respective roles of the two rounds of DNA replication in ZGA, we analyzed the expression of four ZGA genes (hsp70.1, eif-1a, muerv and zscan4d) in embryos whose DNA replication was inhibited by treatment with aphidicolin, an inhibitor of DNA polymerase. Inhibiting the first round increased the expression levels of hsp70.1, eif-1a and zscan4d but decreased that of muerv, while inhibiting the second round increased the expression levels of all four genes. These results suggest that the transcriptionally repressive state seems to be established after the second round of DNA replication.
Sonehara
Hiroki
H
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Chiba, Japan.
Nagata
Masao
M
Aoki
Fugaku
F
D000818:Animals; D016590:Aphidicolin / Q000494:pharmacology; D002970:Cleavage Stage, Ovum / Q000187:drug effects / Q000502:physiology; D004261:DNA Replication / Q000187:drug effects / Q000502:physiology*; D047108:Embryonic Development / Q000187:drug effects / Q000235:genetics; D015977:Eukaryotic Initiation Factor-1 / Q000235:genetics / Q000378:metabolism; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000502:physiology*; D050437:Genes, Developmental / Q000187:drug effects / Q000502:physiology; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D019384:Nucleic Acid Synthesis Inhibitors; D011247:Pregnancy; D011506:Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D015053:Zygote / Q000187:drug effects / Q000378:metabolism*
D015977:Eukaryotic Initiation Factor-1; D018840:HSP70 Heat-Shock Proteins; D051741:Kruppel-Like Transcription Factors; C499132:MuERV-L protein, mouse; D019384:Nucleic Acid Synthesis Inhibitors; D011506:Proteins; D014157:Transcription Factors; C534888:Zscan4d protein, mouse; C026022:eukaryotic peptide initiation factor-1A; C081884:heat-shock protein 70.1; D016590:Aphidicolin