Immobilization of glucose oxidase on polymer membranes treated by low-temperature plasma.
Low-temperature plasma was employed for activation of polymer membranes as a carrier for enzyme immobilization. Glucose oxidase was immobilized on polypropylene (PP), polyvinylidene fluoride (PVDF), or polytetrafluoroethylene (PTFE) membrane surfaces treated by nitrogen or ammonia gas plasma using glutaraldehyde as a linking agent. Enzyme activity was evaluated by the response of glucose sensor composed of the immobilized enzyme membrane and a dissolved oxygen electrode. The sensor response was found to depend on the kind of carrier membrane and to become maximum at suitable conditions of plasma treatment.
Kawakami
M
M
Department of Electronic Material Engineering, Fukuoka Institute of Technology, Fukuoka 811-02, Japan.
Koya
H
H
Gondo
S
S