title: Human coronary artery smooth muscle cell response to a novel PLA textile/fibrin gel composite scaffold.
abstract: Previous studies have demonstrated the potential of fibrin as a cell carrier for cardiovascular tissue engineering applications. Unfortunately, fibrin exhibits poor mechanical properties. One method of addressing this issue is to incorporate a textile in fibrin to provide structural support. However, it is first necessary to develop a deeper understanding of the effect of the textile on cell response. In this study, the cytotoxicity of a polylactic acid (PLA) warp-knit textile was assessed with human coronary artery smooth muscle cells (HCASMC). Subsequently, quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) was employed to examine the gene expression of HCASMC embedded in fibrin with and without the textile. Five genes were examined over a 3-week period: smooth muscle alpha-actin (SMalphaA), myosin heavy chain 11 smooth muscle (SM1/SM2), calponin, myosin heavy chain 10 non-muscle (SMemb) and collagen. Additionally, a microarray analysis was performed to examine a wider range of genes. The knitting process did not adversely affect the cell response; there was no dramatic change in cell number or metabolic rate compared to the negative control. After 3 weeks, there was no significant difference in gene expression, except for a slight decrease of 10% in SMemb in the fibrin with textile. After 3 weeks, there were no obvious cytotoxic effects observed as a result of the knitting process and the gene expression profile did not appear to be altered in the presence of the mesh in the fibrin gel.
authors.affiliation: National Centre for Biomedical Engineering Science, National University of Ireland, Newcastle Road, Galway, Ireland. sarah.gundy@nuigalway.ie
authors.forename: Sarah
authors.initials: S
authors.lastname: Gundy
authors.forename: Grainne
authors.initials: G
authors.lastname: Manning
authors.forename: Enda
authors.initials: E
authors.lastname: O'Connell
authors.forename: Ville
authors.initials: V
authors.lastname: Ellä
authors.forename: Marvi Sri
authors.initials: MS
authors.lastname: Harwoko
authors.forename: Yuri
authors.initials: Y
authors.lastname: Rochev
authors.forename: Terry
authors.initials: T
authors.lastname: Smith
authors.forename: Valerie
authors.initials: V
authors.lastname: Barron
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D002470:Cell Survival; D003094:Collagen / Q000737:chemistry; D003331:Coronary Vessels / Q000473:pathology*; D005109:Extracellular Matrix / Q000378:metabolism; D005337:Fibrin / Q000737:chemistry; D005786:Gene Expression Regulation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000737:chemistry*; D008840:Microfilament Proteins / Q000378:metabolism; D032389:Myocytes, Smooth Muscle / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis; D011091:Polyesters; D011108:Polymers / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013718:Tensile Strength; D000096985:Calponins
chemical_list: D001672:Biocompatible Materials; D002135:Calcium-Binding Proteins; D003094:Collagen; D005337:Fibrin; D019344:Lactic Acid; D008840:Microfilament Proteins; D011091:Polyesters; D011108:Polymers; D000096985:Calponins; C033616:poly(lactide)