title: Spore production of Penicillium roqueforti by solid state fermentation: Stoichiometry, growth and sporulation behavior.
abstract: The development of Penicillium roqueforti on buckwheat seeds proceeds roughly into four steps, involving a lag phase and three growth phases. First, it appears as a spore germination and external colonization of the grains by the mycelium. Then, mainly external sporulation and internal colonization of the seeds occur and finally internal sporulation takes place. The Stoichiometry of the growth and the sporulation is established. Kinetic experiments performed in a fixed bed reactor show that the growth of the microorganism (biomass production) may be estimated by the protein content of the medium. This growth occurs with a very low mu(max) value close to 0.030 h(-1). The chitin content of the medium is an indicator of the sporulation, just as the metabolic liquor (mainly water) produced during the course of a cultivation. The values of the observed respiratory quotient are close to those predicted by stoichiometry.
authors.affiliation: Laboratoire de Génie Chimique Biologique Université de Clermont II, B.P. 45, 63170 Aubiere, France.
authors.forename: C
authors.initials: C
authors.lastname: Desfarges
authors.forename: C
authors.initials: C
authors.lastname: Larroche
authors.forename: J B
authors.initials: JB
authors.lastname: Gros