Methods for human CD8(+) T lymphocyte proteome analysis.
T lymphocytes, including cytotoxic CD8(+) T cells, are important cells involved in immunology, as they can destroy infected or tumor cells. We describe here a detailed protocol starting from CD8(+) T lymphocytes isolation for T cell culture followed by total protein extraction or subcellular fractionation, like nuclei isolation. We also describe well-defined biochemistry and cell biology methods adapted to T lymphocytes, showing the importance of using the method best suited to answering the question addressed. All these techniques would be very helpful to immunologists willing to study underlying biological processes related to T lymphocytes.
Thadikkaran
Lynne
L
Service Régional Vaudois de Transfusion Sanguine, Lausanne, Switzerland.
Rufer
Nathalie
N
Benay
Corinne
C
Crettaz
David
D
Tissot
Jean-Daniel
JD
D015153:Blotting, Western / Q000379:methods; D018414:CD8-Positive T-Lymphocytes / Q000737:chemistry* / Q000166:cytology; D018929:Cell Culture Techniques; D002458:Cell Fractionation / Q000295:instrumentation / Q000379:methods*; D002467:Cell Nucleus / Q000737:chemistry; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D008969:Molecular Sequence Data; D020543:Proteome / Q000032:analysis*
D020543:Proteome