Extractive bioconversions in aqueous two-phase systems: enzymatic hydrolysis of casein proteins.
Partition coefficients in poly(ethylene glycol)/dextran aqueous two-phase systems are reported for mixed-casein and its components, alpha, beta and kappa casein. Rates of casein proteolysis by alpha-chymotrypsin and by trypsin are reported in single-phase and aqueous two-phase reactor systems. The advantages resulting from selective partitioning of substrates, enzymes, and products are examined in terms of relative volumetric reaction rates.
Mukataka
S
S
Department of Chemical Engineering, University of California, Berkeley, California 94720, USA.
Haynes
C A
CA
Prausnitz
J M
JM
Blanch
H W
HW