Effective radiotherapy of primary tumors and metastasis with 18F-2-deoxy-2-fluoro-D-glucose in C57BL/6 mice.
BACKGROUND
The radiochemical 18F-2-deoxy-2-fluoro-D-glucose (18F-FDG), a positron emitter, is taken up preferentially by malignant tumors with high metabolic rates. This concentration of 18F-FDG in the tumor permits diagnosis and staging by positron emission tomography but also may represent a means of targeting radiotherapy. In this study, we determined the effect of a higher dose of 18F-FDG on tumor growth in a mouse model.


METHODS
The effect of 18F-FDG on the growth and viability of 3 tumor cell lines was determined in vitro. Primary tumor growth and metastasis of B16/BL6 melanoma cells were determined in C57BL/6 mice injected with 5 mCi doses of 18F-FDG (2/3 doses).


RESULTS
18F-FDG was cytostatic for all 3 cell lines at the lowest dose tested. It significantly reduced the growth of primary tumors, by 89% at day 19 postinoculation, and also almost totally inhibited the appearance of lung metastases after intravenous inoculation of the same cells.


CONCLUSIONS
18F-FDG proved to be an effective radiotherapeutic agent in this model. The possible problems associated with the accumulation of this radiochemical at other sites besides the tumor must be addressed.
Caridad
Victoria
V
Laboratorio de Patología Celular y Molecular, Centro de Medicina Experimental, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela.
Arsenak
Miriam
M
Abad
María Jesús
MJ
Martín
Rafael
R
Guillén
Nilo
N
Colmenter
Luís Felipe
LF
Taylor
Peter
P
D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000494:pharmacology*; D005840:Gentian Violet / Q000494:pharmacology; D006801:Humans; D008175:Lung Neoplasms / Q000175:diagnosis / Q000473:pathology; D008546:Melanoma, Experimental; D051379:Mice; D008810:Mice, Inbred C57BL; D009362:Neoplasm Metastasis; D009369:Neoplasms / Q000188:drug therapy* / Q000532:radiotherapy*; D049268:Positron-Emission Tomography / Q000379:methods; D018787:Radiation Oncology / Q000379:methods; D019275:Radiopharmaceuticals / Q000494:pharmacology*
D000970:Antineoplastic Agents; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; D005840:Gentian Violet