Processing scarce biological samples for light and transmission electron microscopy.
Light microscopy (LM) and transmission electron microscopy (TEM) aim at understanding the relationship structure-function. With advances in biology, isolation and purification of scarce populations of cells or subcellular structures may not lead to enough biological material, for processing for LM and TEM. A protocol for preparation of scarce biological samples is presented. It is based on pre-embedding the biological samples, suspensions or pellets, in bovine serum albumin (BSA) and bis-acrylamide (BA), cross-linked and polymerized. This preparation provides a simple and reproducible technique to process biological materials, present in limited quantities that can not be amplified, for light and transmission electron microscopy.
Taupin
Philippe
P
National Neuroscience Institute, Singapore. obgpjt@nus.edu.sg
D016475:3T3 Cells; D000818:Animals; D016591:Histocytological Preparation Techniques; D051379:Mice; D008853:Microscopy / Q000379:methods*; D008854:Microscopy, Electron