Development of an SPME-GC-MS/MS procedure for the monitoring of 2-phenoxyethanol in anaesthetised fish.
2-Phenoxyethanol (ethylene glycol monophenyl ether, C(8)H(10)O(2)) is a promising anaesthetic agent used in fisheries and aquaculture. The aim of this study was to develop a fast and easy method to determine 2-phenoxyethanol residue levels in fish tissue and blood plasma, and, subsequently, to use the method to monitor the dynamics of 2-phenoxyethanol residues in fish treated with anaesthetic. We developed a new procedure that employs solid phase microextraction (SPME) of the target analyte from the sample headspace followed by gas chromatography-mass spectrometry (GC-MS). Both sample handling, aimed at maximum transfer of 2-phenoxyethanol into the headspace, and SPME-GC-MS conditions were carefully optimised. Using a divinylbenzene/Carboxen/polydimethylsiloxane (PDMS/CAR/DVB) fiber for 60 min sampling at 30 degrees C and an ion trap detector operated in MS/MS mode, we obtained detection (LOD) and quantification (LOQ) limits of 0.03 and 0.1 mg kg(-1) of sample, respectively. The method was linear in a range of 0.1-250 mg kg(-1) and, depending on the sample matrix and spiking level, a repeatability (expressed as relative standard deviation, R.S.D.) of between 3% and 11% was obtained.
Klimánková
Eva
E
Institute of Chemical Technology, Faculty of Food and Biochemical Technology, Department of Food Chemistry and Analysis, Technická 3, 166 28 Prague 6, Czech Republic.
Riddellová
Katerina
K
Hajslová
Jana
J
Poustka
Jan
J
Kolárová
Jitka
J
Kocourek
Vladimír
V
D000777:Anesthetics / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D000818:Animals; D005026:Ethylene Glycols / Q000008:administration & dosage / Q000032:analysis* / Q000493:pharmacokinetics; D005399:Fishes / Q000378:metabolism*; D008401:Gas Chromatography-Mass Spectrometry; D009132:Muscles / Q000737:chemistry / Q000166:cytology; D015203:Reproducibility of Results; D052617:Solid Phase Microextraction / Q000379:methods*
D000777:Anesthetics; D005026:Ethylene Glycols; C005398:phenoxyethanol