Gene targeting of CK2 catalytic subunits.
Protein kinase CK2 is a highly conserved and ubiquitous serine-threonine kinase. It is a tetrameric enzyme that is made up of two regulatory CK2beta subunits and two catalytic subunits, either CK2alpha/CK2alpha, CK2alpha/CK2alpha', or CK2alpha'/CK2alpha'. Although the two catalytic subunits diverge in their C termini, their enzymatic activities are similar. To identify the specific function of the two catalytic subunits in development, we have deleted them individually from the mouse genome by homologous recombination. We have previously reported that CK2alpha' is essential for male germ cell development, and we now demonstrate that CK2alpha has an essential role in embryogenesis, as mice lacking CK2alpha die in mid-embryogenesis, with cardiac and neural tube defects.
Seldin
David C
DC
Department of Medicine, Boston University School of Medicine and Boston Medical Center, 650 Albany Street, Boston, MA 02118, USA. dseldin@bu.edu
Lou
David Y
DY
Toselli
Paul
P
Landesman-Bollag
Esther
E
Dominguez
Isabel
I
D000483:Alleles; D000818:Animals; D047390:Casein Kinase II / Q000172:deficiency / Q000235:genetics*; D020134:Catalytic Domain / Q000235:genetics*; D004622:Embryo, Mammalian / Q000002:abnormalities / Q000201:enzymology; D018390:Gene Targeting; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D009436:Neural Tube Defects / Q000201:enzymology; D013623:Tail / Q000002:abnormalities / Q000196:embryology
D047390:Casein Kinase II