Chromosome pulverization in virus-induced heterokaryons of mammalian cells from different species.
The phenomena of cell fusion and chromosome pulverization after inoculation with UV-inactivated Sendai virus were studied in mixed suspensions of cell lines from different mammalian species. Two cell combinations were used: the Chinese hamster cell line (Don) and RPMI 8226 (Simpson) cell line of human hematopoietic origin,and the Don and LLC-MK2 (MK) cell lines, the latter derived from monkey kidney cells. Each of the 3 cell lines had a high cell-fusion capacity when infected with the virus in homologous cultures. The heterokaryons of Don and Simpson cells were formed at lower frequency than homokaryons in either Don or Simpson cells, and heterokaryon formation between Don and MK cells was rare. The findings indicated that the cells used maintained some specificity of the cell surfaces. Chromosome pulverization was found in heterokaryons and was similar to that observed in homokaryons. The results confirmed further our presently held concept that cell fusion is a prerequisite for induction of pulverization, and also suggest that the possible role played by the mitotic nucleus with its cytoplasm in pulverization applies to the heterologous as well as to the homologous nuclei.
Ikeuchi
T
T
Park Memorial Institute, Buffalo, New York 14203, USA.
Sandberg
A A
AA
D000818:Animals; D002459:Cell Fusion; D002460:Cell Line; D002467:Cell Nucleus / Q000235:genetics*; D048750:Cell Nucleus Division / Q000235:genetics*; D002877:Chromosomes, Human; D033481:Chromosomes, Mammalian; D006224:Cricetinae; D003412:Cricetulus; D006410:Hematopoiesis; D006801:Humans; D007668:Kidney / Q000166:cytology; D008938:Mitosis; D011208:Powders; D029082:Sendai virus; D013045:Species Specificity; D014466:Ultraviolet Rays; D038301:Virus Inactivation
D011208:Powders