title: Immunohistochemical detection of insulin-like growth factor-I, transforming growth factor-beta2, basic fibroblast growth factor and epidermal growth factor-receptor expression in developing rat ovary.
abstract: The aim of this study was to determine the immunohistochemical expression and localization of insulin-like growth factor-I (IGF-I), transforming growth factor-beta2 (TGF-beta2), basic fibroblast growth factor (bFGF) and epidermal growth factor-receptor (EGF-R) in developing rat ovaries. Eighteen female Wistar rats were enrolled in this study; newborn (n=6), one-month-old (n=6) and adult (n=6) rats. Formalin-fixed and parafin-embedded ovarian tissues were stained with antibodies against IGF-I, TGF-beta2, bFGF and EGF-R, immunohistochemically. The ovarian cells were evaluated by semi-quantitative scoring system under light microscope. The staining of IGF-I, TGF-beta2, bFGF and EGF-R were most intense in the oocytes and were heavily at one-month-old rats. A moderate immunostaining in theca cells and corpus luteii reacted with IGF-I in adult rats. Furthermore the staining intensity for IGF-I was moderate in granulosa cells of newborn rat ovaries. We detected also a moderate staining for TGF-beta2 in corpus luteii of adult rats. In addition, we found a bFGF immunostaining mainly in oocytes of follicles of young and adult rats. Immunostaining for EGF-R was moderate in granulosa cells of one-month-old rats. In conclusion, this study suggests that growth factors play a pivotal role in ovarian function, especially in follicular development. The role of growth factor in controlling degeneration or growth (or both) of ovary follicles remain as explained.
authors.affiliation: Department of Histology and Embryology, Adnan Menderes University School of Medicine, TR-09100 Aydin, Turkey.
authors.forename: K
authors.initials: K
authors.lastname: Ergin
authors.forename: E
authors.initials: E
authors.lastname: Gürsoy
authors.forename: Yücel
authors.initials: Y
authors.lastname: Başimoğlu Koca
authors.forename: H
authors.initials: H
authors.lastname: Başaloğlu
authors.forename: K
authors.initials: K
authors.lastname: Seyrek
mesh_terms: D000818:Animals; D066246:ErbB Receptors / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism*; D007150:Immunohistochemistry; D007334:Insulin-Like Growth Factor I / Q000378:metabolism*; D010053:Ovary / Q000254:growth & development* / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D053781:Transforming Growth Factor beta2 / Q000378:metabolism*
chemical_list: D053781:Transforming Growth Factor beta2; D016222:Fibroblast Growth Factor 2; D007334:Insulin-Like Growth Factor I; D066246:ErbB Receptors