The naturally occurring flavolignan, deoxypodophyllotoxin, inhibits lipopolysaccharide-induced iNOS expression through the NF-kappaB activation in RAW264.7 macrophage cells.
Deoxypodophyllotoxin (DPT), a naturally occurring flavolignan with anti-inflammatory activity, was isolated from Anthriscus sylvestris HOFFM., and we examined its effects on the expression of inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-stimulated, murine macrophage-like RAW264.7 cells. Western blot analysis performed with specific anti-iNOS antibodies showed that a decrease in nitric oxide (NO) was accompanied by a decrease in the iNOS protein level. To clarify the mechanistic basis for DPT's ability to inhibit iNOS induction, we examined the effect of DPT on nuclear factor (NF)-kappaB transcriptional activity and DNA binding activity. DPT potently suppressed both reporter gene activity and DNA binding activity. These findings suggest that DPT in RAW264.7 cells abolished LPS-induced iNOS expression by inhibiting the transcription factor, NF-kappaB.
Jin
Meihua
M
College of Pharmacy, Yeungnam University, Gyeongsan, Republic of Korea.
Moon
Tae Chul
TC
Quan
Zhejiu
Z
Lee
Eunkyung
E
Kim
Yun Kyung
YK
Yang
Ju Hae
JH
Suh
Seok-Jong
SJ
Jeong
Tae Cheon
TC
Lee
Seung Ho
SH
Kim
Cheorl-Ho
CH
Chang
Hyeun Wook
HW
D000818:Animals; D019661:Apiaceae / Q000737:chemistry; D015153:Blotting, Western; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D002855:Chromatography, Thin Layer; D004247:DNA / Q000378:metabolism; D004365:Drugs, Chinese Herbal; D004591:Electrophoresis, Polyacrylamide Gel; D024202:Electrophoretic Mobility Shift Assay; D007202:Indicators and Reagents; D008070:Lipopolysaccharides / Q000037:antagonists & inhibitors* / Q000633:toxicity; D008156:Luciferases / Q000235:genetics; D008264:Macrophages / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis*; D018517:Plant Roots / Q000737:chemistry; D011034:Podophyllotoxin / Q000031:analogs & derivatives* / Q000494:pharmacology; D051996:Transcription Factor RelA / Q000378:metabolism*; D014162:Transfection
D004365:Drugs, Chinese Herbal; D007202:Indicators and Reagents; D008070:Lipopolysaccharides; D051996:Transcription Factor RelA; C014451:deoxypodophyllotoxin; D004247:DNA; D008156:Luciferases; D052247:Nitric Oxide Synthase Type II; D011034:Podophyllotoxin