title: PCR detection of type I polyketide synthase genes in myxobacteria.
abstract: The diversity of type I modular polyketide synthase (PKS) was explored by PCR amplification of DNA encoding ketosynthase and acyltransferase domains in myxobacteria. The sequencing of the amplicons revealed that many PKS genes were distantly related to the published sequences. Thus, myxobacteria may be excellent resources for novel and diverse polyketides.
authors.affiliation: NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan. hskomaki@nbrc.nite.go.jp
authors.forename: Hisayuki
authors.initials: H
authors.lastname: Komaki
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Fudou
authors.forename: Takashi
authors.initials: T
authors.lastname: Iizuka
authors.forename: Daisuke
authors.initials: D
authors.lastname: Nakajima
authors.forename: Koei
authors.initials: K
authors.lastname: Okazaki
authors.forename: Daisuke
authors.initials: D
authors.lastname: Shibata
authors.forename: Makoto
authors.initials: M
authors.lastname: Ojika
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Harayama
mesh_terms: D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D014644:Genetic Variation; D008969:Molecular Sequence Data; D009231:Myxococcales / Q000201:enzymology / Q000235:genetics*; D010802:Phylogeny; D048630:Polyketide Synthases / Q000235:genetics*; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D048630:Polyketide Synthases