Saline infusion through the pancreatic duct leads to changes in calcium homeostasis similar to those observed in acute pancreatitis.
This work focuses on studying the early events associated with pancreatic damage after retrograde infusion through the pancreatic duct in rats. We have analyzed changes in calcium homeostasis and secretory response in pancreatic acini from rats with taurocholate-induced acute pancreatitis. Moreover, in order to test whether pancreatic duct manipulation can trigger damage inside pancreatic acinar cells, we have studied both parameters in acini from animals infused with saline. Our study demonstrates that taurocholate causes evident damage to acinar cells, impairing both calcium homeostasis and secretory response to CCK. In saline, a significant decrease in calcium cytosolic response to CCK was observed. Calcium disturbances similar to those observed in acute pancreatitis appear before secretion blockade and inflammation processes in saline treated rats. These results could be interesting since pancreatitis is associated to clinical procedures that require duct manipulation such as endoscopic retrograde cholangiopancreatography.
García
Mónica
M
Department of Physiology and Pharmacology, Edificio Departamental, University of Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain.
Barbáchano
Ernesto Hernández
EH
Lorenzo
Pilar Hernández
PH
San Román
José Ignacio
JI
López
María A
MA
Coveñas
Rafael
R
Calvo
José Julián
JJ
D000681:Amylases / Q000378:metabolism; D000818:Animals; D015740:Calcitonin Gene-Related Peptide / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D002760:Cholangiopancreatography, Endoscopic Retrograde / Q000009:adverse effects; D002766:Cholecystokinin / Q000378:metabolism*; D003600:Cytosol / Q000378:metabolism; D006706:Homeostasis; D008297:Male; D010179:Pancreas / Q000473:pathology; D010183:Pancreatic Ducts / Q000293:injuries*; D019283:Pancreatitis, Acute Necrotizing / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012965:Sodium Chloride / Q000008:administration & dosage; D013373:Substance P / Q000378:metabolism; D013656:Taurocholic Acid / Q000008:administration & dosage* / Q000633:toxicity
D013373:Substance P; D012965:Sodium Chloride; D013656:Taurocholic Acid; D002766:Cholecystokinin; D000681:Amylases; D015740:Calcitonin Gene-Related Peptide; D002118:Calcium