[Expression and localization of hnRNP A2/B1 during differentiation of human osteosarcoma MG-63 cells induced by HMBA].
OBJECTIVE
The differentially expressed nuclear matrix proteins have great effects on canceration and regulation of cell differentiation. This study was to explore the existence and distribution of ribonucleoprotein hnRNP A2/B1 in nuclear matrix and its co-localization with Actin and Prohibitin in human osteosarcoma MG-63 cells before and after hexamethylene bisacetamide (HMBA) treatment.


METHODS
The nuclear matrix of MG-63 cells before and after treatment of HMBA were selectively extracted. The expression and localization of hnRNP A2/B1 in nuclear matrix were detected by 2-D PAGE, MALDI-TOF-MS, Western blot, and immunofluorescent staining. The co-localization of hnRNP A2/B1 with Actin and Prohibitin was observed under laser scanning confocal microscope (LSCM).


RESULTS
hnRNP A2/B1 was detected in the component of nuclear matrix proteins of MG-63 cells by Western blot and immunogold staining and its expression was decreased after treatment of HMBA. hnRNP A2/B1 was located in the nuclear matrix, and its expression was weakened after HMBA treatment. hnRNP A2/B1 was co-localized with Actin or Prohibitin in MG-63 cells, while the co-localization relationship was weakened during differentiation of MG-63 cells.


CONCLUSIONS
hnRNP A2/B1 is a kind of nuclear matrix protein, and localizes in the nuclear matrix. The distribution and expression of hnRNP A2/B1 and its co-localization with Actin and Prohibitin play important roles during the differentiation of MG-63 cells.
Zhao
Chun-Hong
CH
Key Laboratory of Education Ministry of China for Cell Biology and Tumor Cell Engineering,School of Life Sciences, Xiamen University, Xiamen, Fujian, 361005, P R China.
Li
Qi-Fu
QF
Chen
Lan-Ying
LY
Tang
Jian
J
Song
Jian-Ye
JY
Xie
Zhong
Z
D000081:Acetamides / Q000494:pharmacology*; D000199:Actins / Q000032:analysis; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001859:Bone Neoplasms / Q000737:chemistry* / Q000473:pathology; D002454:Cell Differentiation; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B / Q000032:analysis* / Q000502:physiology; D006801:Humans; D015530:Nuclear Matrix / Q000737:chemistry; D012516:Osteosarcoma / Q000737:chemistry* / Q000473:pathology; D000091402:Prohibitins; D012097:Repressor Proteins / Q000032:analysis; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000081:Acetamides; D000199:Actins; D000970:Antineoplastic Agents; D034481:Heterogeneous-Nuclear Ribonucleoprotein Group A-B; D000091402:Prohibitins; D012097:Repressor Proteins; C439768:hnRNP A2; C014026:hexamethylene bisacetamide