6-Shogaol and 6-gingerol, the pungent of ginger, inhibit TNF-alpha mediated downregulation of adiponectin expression via different mechanisms in 3T3-L1 adipocytes.
In this study, we demonstrated that the two ginger-derived components have a potent and unique pharmacological function in 3T3-L1 adipocytes via different mechanisms. Both pretreatment of 6-shogaol (6S) and 6-gingerol (6G) significantly inhibited the tumor necrosis factor-alpha (TNF-alpha) mediated downregulation of the adiponectin expression in 3T3-L1 adipocytes. Our study demonstrate that (1) 6S functions as a PPARgamma agonist with its inhibitory mechanism due to the PPARgamma transactivation, and (2) 6G is not a PPARgamma agonist, but it is an effective inhibitor of TNF-alpha induced c-Jun-NH(2)-terminal kinase signaling activation and thus, its inhibitory mechanism is due to this inhibitory effect.
Isa
Yasuka
Y
College of Bioscience and Biotechnology, Chubu University, Kasugai, 1200 Matsumoto-cho, Kasugai, Aichi 487-8501, Japan.
Miyakawa
Yuri
Y
Yanagisawa
Masayoshi
M
Goto
Tsuyoshi
T
Kang
Min-Sook
MS
Kawada
Teruo
T
Morimitsu
Yasujiro
Y
Kubota
Kikue
K
Tsuda
Takanori
T
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D052242:Adiponectin / Q000096:biosynthesis*; D000818:Animals; D002396:Catechols / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D005233:Fatty Alcohols / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects; D020939:Zingiber officinale / Q000737:chemistry; D048031:JNK Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors; D051379:Mice; D047495:PPAR gamma / Q000819:agonists; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000494:pharmacology
D052242:Adiponectin; D002396:Catechols; D005233:Fatty Alcohols; D047495:PPAR gamma; D014409:Tumor Necrosis Factor-alpha; C040115:shogaol; C007845:gingerol; D048031:JNK Mitogen-Activated Protein Kinases