title: Repair of UVB-damaged skin by the antioxidant sulphated flavone glycoside thalassiolin B isolated from the marine plant Thalassia testudinum Banks ex König.
abstract: Daily topical application of the aqueous ethanolic extract of the marine sea grass, Thalassia testudinum, on mice skin exposed to UVB radiation resulted in a dose-dependent recovery of the skin macroscopic alterations over a 6-day period. Maximal effect (90%) occurred at a dose of 240 microg/cm(2), with no additional effects at higher doses. Bioassay-guided fractionation of the plant extract resulted in the isolation of thalassiolin B (1). Topical application of 1 (240 microg/cm(2)) markedly reduces skin UVB-induced damage. In addition, thalassiolin B scavenged 2,2-diphenyl-2-picrylhydrazyl radical with an EC(50) = 100 microg/ml. These results suggest that thalassiolin B is responsible for the skin-regenerating effects of the crude extract of T. testudinum.
authors.affiliation: Department of Chemistry, Center of Marine Bioproducts, Loma y 37, Alturas del Vedado, C.P. 10400 Havana, Cuba. erikluis18@gmail.com
authors.forename: Erik L
authors.initials: EL
authors.lastname: Regalado
authors.forename: María
authors.initials: M
authors.lastname: Rodríguez
authors.forename: Roberto
authors.initials: R
authors.lastname: Menéndez
authors.forename: Angel A
authors.initials: AA
authors.lastname: Concepción
authors.forename: Clara
authors.initials: C
authors.lastname: Nogueiras
authors.forename: Abilio
authors.initials: A
authors.lastname: Laguna
authors.forename: Armando A
authors.initials: AA
authors.lastname: Rodríguez
authors.forename: David E
authors.initials: DE
authors.lastname: Williams
authors.forename: Patricia
authors.initials: P
authors.lastname: Lorenzo-Luaces
authors.forename: Olga
authors.initials: O
authors.lastname: Valdés
authors.forename: Yasnay
authors.initials: Y
authors.lastname: Hernandez
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D031321:Hydrocharitaceae / Q000737:chemistry*; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D015394:Molecular Structure; D011833:Radiation Injuries, Experimental / Q000188:drug therapy; D012867:Skin / Q000187:drug effects* / Q000473:pathology / Q000528:radiation effects*; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D005419:Flavonoids; C471629:thalassiolin B