Silanizing agent for semipermanent wall coating in micellar electrokinetic capillary chromatography.
In this paper, the long-chained, silanizing agent chloro(dodecyl)dimethylsilane (CDDS) was investigated as a semipermanent coating in micellar electrokinetic capillary chromatography (MEKC). CDDS coating had great stability due to the formation of covalent bonding with the silanol groups on the surface of fused-silica capillary and remained stable for over 100 min after removal of the rinse step of CDDS solution. Anionic surfactant sodium dodecyl sulfate (SDS) could aggregate at this CDDS coating by the hydrophobic group and formed a SDS layer which could increase the electroosmotic flow (EOF). The separation was performed with the running buffer composed of 60mM sodium tetraborate, 12 mM SDS at pH 9.9, with the applied voltage of 20 kV and capillary temperature 25 degrees C. The effect of the coating agent was investigated by the analysis of amino acids. Compared with previous no-coating method, the EOF increases from 4.34 x 10(-4)cm(2)V(-1)s(-1) to 7.02 x 10(-4)cm(2)V(-1)s(-1). Migration time reproducibility was less than 0.97% R.S.D. from run to run and less than 1.56% R.S.D. from day to day.
Luo
Zhi
Z
Department of Chemistry, Lanzhou University, Tianshui Road 298#, Lanzhou 730000, PR China. luozh03@lzu.cn
Wang
Shumin
S
Zhou
Lei
L
Hu
Zhide
Z
D000596:Amino Acids / Q000032:analysis; D002021:Buffers; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000295:instrumentation* / Q000592:standards; D015203:Reproducibility of Results; D012821:Silanes; D012967:Sodium Dodecyl Sulfate; D013499:Surface Properties
D000596:Amino Acids; D002021:Buffers; D012821:Silanes; D012967:Sodium Dodecyl Sulfate