MicroRNA-126 inhibits invasion in non-small cell lung carcinoma cell lines.
Crk is a member of a family of adaptor proteins that are involved in intracellular signal pathways altering cell adhesion, proliferation, and migration. Increased expression of Crk has been described in lung cancer and associated with increased tumor invasiveness. MicroRNAs (miRNAs) are a family of small non-coding RNAs (approximately 21-25 nt long) that are capable of targeting genes for either degradation of mRNA or inhibition of translation. Crk is a predicted putative target gene for miR-126. Over-expression of miR126 in a lung cancer cell line resulted in a decrease in Crk protein without any alteration in the associated mRNA. These lung cancer cells exhibit a decrease in adhesion, migration, and invasion. Decreased cancer cell invasion was also evident following targeted knockdown of Crk. MiR-126 alters lung cancer cell phenotype by inhibiting adhesion, migration, and invasion and the effects on invasion may be partially mediated through Crk regulation.
Crawford
M
M
The Ohio State University Medical Center, Division of Pulmonary, Allergy, Critical Care and Sleep Medicine, DHLRI 473 West 12th Avenue Room 201, Columbus, OH 43210, USA.
Brawner
E
E
Batte
K
K
Yu
L
L
Hunter
M G
MG
Otterson
G A
GA
Nuovo
G
G
Marsh
C B
CB
Nana-Sinkam
S P
SP
D002289:Carcinoma, Non-Small-Cell Lung / Q000378:metabolism / Q000473:pathology*; D002448:Cell Adhesion / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009361:Neoplasm Invasiveness; D050737:Proto-Oncogene Proteins c-crk / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism
C485285:CRK protein, human; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D050737:Proto-Oncogene Proteins c-crk; D012333:RNA, Messenger