title: Proteomic analysis of lamellar bodies isolated from rat lungs.
abstract: BACKGROUND
  Lamellar bodies are lysosome-related secretory granules and store lung surfactant in alveolar type II cells. To better understand the mechanisms of surfactant secretion, we carried out proteomic analyses of lamellar bodies isolated from rat lungs.
  
  
  RESULTS
  With peptide mass fingerprinting by Matrix Assisted Laser Desorption/Ionization - Time of Flight mass spectrometry, 44 proteins were identified with high confidence. These proteins fell into diverse functional categories: surfactant-related, membrane trafficking, calcium binding, signal transduction, cell structure, ion channels, protein processing and miscellaneous. Selected proteins were verified by Western blot and immunohistochemistry.
  
  
  CONCLUSIONS
  This proteomic profiling of lamellar bodies provides a basis for further investigations of functional roles of the identified proteins in lamellar body biogenesis and surfactant secretion.
authors.affiliation: Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078, USA. pengcheng.wang@okstate.edu
authors.forename: Pengcheng
authors.initials: P
authors.lastname: Wang
authors.forename: Narendranath Reddy
authors.initials: NR
authors.lastname: Chintagari
authors.forename: Jeyaparthasarathy
authors.initials: J
authors.lastname: Narayanaperumal
authors.forename: Sahlu
authors.initials: S
authors.lastname: Ayalew
authors.forename: Steven
authors.initials: S
authors.lastname: Hartson
authors.forename: Lin
authors.initials: L
authors.lastname: Liu
mesh_terms: D000818:Animals; D008168:Lung / Q000648:ultrastructure*; D011506:Proteins / Q000032:analysis* / Q000502:physiology; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D011663:Pulmonary Surfactants / Q000378:metabolism; D051381:Rats; D022142:Secretory Vesicles / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D011506:Proteins; D020543:Proteome; D011663:Pulmonary Surfactants