Combinatorial and automated synthesis of phosphodiester galactosyl cluster on solid support by click chemistry assisted by microwaves.
Small libraries of di-, tri-, and tetragalactosyl clusters were efficiently synthesized using combinatorial methodology, on solid support, by click chemistry assisted by microwaves, starting from different poly alkyne DNA-based scaffolds and two galactosyl azide derivatives. The scaffold was synthesized by standard DNA solid-supported phosphoramidite chemistry using a novel alkyne phosphoramidite and an alkyne solid support. The proportion of each glycocluster in a library was modulated using different molar ratios of both galactose azides.
Pourceau
Gwladys
G
Institut des Biomolécules Max Mousseron, UMR 5247 CNRS-Université Montpellier 1, Place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France.
Meyer
Albert
A
Vasseur
Jean-Jacques
JJ
Morvan
François
F
D000107:Acetylation; D001386:Azides / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D020650:Combinatorial Chemistry Techniques; D004952:Esters / Q000737:chemistry*; D005690:Galactose / Q000138:chemical synthesis* / Q000737:chemistry; D008872:Microwaves; D015394:Molecular Structure; D017553:Phosphorus Compounds / Q000138:chemical synthesis* / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D001386:Azides; D004952:Esters; D017553:Phosphorus Compounds; D005690:Galactose