title: Association of polymorphisms in the human IFN-gamma and IL-4 gene with oral lichen planus: a study in an ethnic Chinese cohort.
abstract: Cytokines have an important role in the pathogenesis and disease progression of oral lichen planus (OLP). The aim of this study was to investigate the impact of gene polymorphisms of T helper cell subtype Th1 and Th2 cytokines, interferon-gamma (IFN-gamma) and interleukin-4 (IL-4), on OLP in an ethnic Chinese cohort. We investigated IFN-gamma gene (IFNG) and IL-4 gene (IL-4) polymorphisms in 151 patients with OLP and 143 healthy controls using the polymerase chain reaction with sequence-specific primers (PCR-SSP) method. Each genotype was also validated by direct DNA sequencing methods to further guarantee the significance of our findings. The data revealed that there were highly significant increases in +874 IFNG2 genotype distributions and allele T frequencies in both the OLP patient group (p = 0.033, 0.003) and the more progressive subtype group (eOLP)(p = 0.013, 0.001) compared with those of normal controls There was no difference between the OLP patient and healthy control groups in frequencies of both the IL-4 allele (p = 0.053) and genotype (p = 0.197), although the frequencies of the IL4-590 C allele (p = 0.001) and the CC genotype (p = 0.024) in patients with nonerosive OLP (neOLP) were significantly greater than the corresponding values in the control group. Our study suggests that IFN-gamma and IL-4 gene polymorphisms seem to have a positive influence on disease susceptibility and progression of OLP in this ethnic Chinese cohort.
authors.affiliation: State Key Laboratory of Oral Diseases, West China College of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Jingping
authors.initials: J
authors.lastname: Bai
authors.forename: Mei
authors.initials: M
authors.lastname: Lin
authors.forename: Xin
authors.initials: X
authors.lastname: Zeng
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Zhang
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.forename: Jun
authors.initials: J
authors.lastname: Shen
authors.forename: Lu
authors.initials: L
authors.lastname: Jiang
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.forename: Qianming
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D002681:China; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D015847:Interleukin-4 / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D017676:Lichen Planus, Oral / Q000235:genetics* / Q000276:immunology; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D018417:Th1 Cells / Q000276:immunology / Q000378:metabolism; D018418:Th2 Cells / Q000276:immunology / Q000378:metabolism
chemical_list: D015847:Interleukin-4; D007371:Interferon-gamma