A possible neural cascade involving the photoneuroendocrine system (PNES) responsible for regulating gonadal development in an avian species, Gallus gallus.
Neurons located in the lateral septal organ (LSO) and medial basal hypothalamus (MBH) have been proposed to be encephalic photoreceptors (EPRs), which sense photoperiodic time and initiate avian gonadal development. Controversy continues regarding the location of EPRs serving the PNES and their signal transduction pathway. Using quantitative real-time RT-PCR we determined activation of key genes following prolonged light periods and sulfamethethazine (compound known to advance light-induced testes development) in 21-day old chicks. Earliest activation occurred in genes of vasoactive intestinal polypeptide (VIP) and type 6 phosphodiesterase beta subunit (PDE-6 beta) in the LSO at 4 and 6h, respectively, after onset of light and sulfamethazine intake. In contrast, no change was detected in the MBH during the first 8h of that treatment. Thereafter, significant increases in gonadotropin releasing hormone (GnRH-1) and VIP receptor (VIPR) mRNA transcripts were detected in the bed nucleus of the pallial commissure (NCPa). Hours later, activation of all four genes (VIP, PDE-6 beta, GnRH-1, VIPR) were induced solely by photostimulation. Deiodinase 2 and tyrosine hydroxylase in the MBH did not show increased gene expression until 12h of photostimulation. Prolactin mRNA transcripts showed significant increases at 4h due to SMZ intake and at 24, 36 and 48 h due to long-day photoperiodic effects. Data suggest that VIP neurons in the LSO may serve as EPRs and utilize PDE, present in the phototransduction cascade of known photoreceptors. Additionally, VIP released from the LSO may modulate GnRH-1 neurons in the NCPa via VIP receptors by increasing GnRH-1 gene expression.
Li
Hongyan
H
Department of Poultry Science, University of Arkansas, Fayetteville, AR 72701, United States. lee74us2001@yahoo.com
Kuenzel
Wayne J
WJ
D000818:Animals; D000890:Anti-Infective Agents / Q000008:administration & dosage / Q000494:pharmacology; D001921:Brain / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D002645:Chickens / Q000235:genetics* / Q000254:growth & development; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6 / Q000235:genetics; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects / Q000528:radiation effects; D007987:Gonadotropin-Releasing Hormone / Q000235:genetics; D007453:Iodide Peroxidase / Q000235:genetics; D008297:Male; D017440:Photoperiod; D010903:Pituitary Gland, Anterior / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism; D011388:Prolactin / Q000235:genetics; D018005:Receptors, Vasoactive Intestinal Peptide / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013418:Sulfamethazine / Q000008:administration & dosage / Q000494:pharmacology*; D013737:Testis / Q000254:growth & development / Q000378:metabolism*; D014446:Tyrosine 3-Monooxygenase / Q000235:genetics; D014660:Vasoactive Intestinal Peptide / Q000235:genetics; D014785:Vision, Ocular / Q000187:drug effects / Q000235:genetics* / Q000528:radiation effects; D000097564:Iodothyronine Deiodinase Type II
D000890:Anti-Infective Agents; D054707:Cyclic Nucleotide Phosphodiesterases, Type 6; D007987:Gonadotropin-Releasing Hormone; D007453:Iodide Peroxidase; D011388:Prolactin; D018005:Receptors, Vasoactive Intestinal Peptide; D013418:Sulfamethazine; D014446:Tyrosine 3-Monooxygenase; D014660:Vasoactive Intestinal Peptide; D000097564:Iodothyronine Deiodinase Type II