title: Characterization of monoclonal antibodies that recognize the Eimeria tenella microneme protein MIC2.
abstract: The apicomplexan pathogens of Eimeria cause coccidiosis, an intestinal disease of chickens, which has a major economic impact on the poultry industry. Members of the Apicomplexa share an assortment of unique secretory organelles (rhoptries, micronemes and dense granules) that mediate invasion of host cells and formation and modification of the parasitophorous vacuole. Among these, microneme protein 2 from Eimeria tenella(EtMIC2) has a putative function in parasite adhesion to the host cell to initiate the invasion process. To investigate the role of EtMIC2 in host parasite interactions, the production and characterization of 12 monoclonal antibodies (mabs) produced against recombinant EtMIC2 proteins is described. All mabs reacted with molecules belonging to the apical complex of sporozoites and merozoites of E. tenella, E. acervulina and E. maxima in an immunofluorescence assay. By Western blot analysis, the mabs identified a developmentally regulated protein of 42 kDa corresponding to EtMIC 2 and cross-reacted with proteins in developmental stages of E. acervulina. Collectively, these mabs are useful tools for the detailed investigation of the characterization of EtMIC2 related proteins in Eimeria species.
authors.affiliation: Department of Beterinary Internal Medicine, Division of beterinary Science, Graduate School of life and Environmental Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
authors.forename: Kazumi
authors.initials: K
authors.lastname: Sasai
authors.forename: Raymond H
authors.initials: RH
authors.lastname: Fetterer
authors.forename: Hyun
authors.initials: H
authors.lastname: Lillehoj
authors.forename: Satomi
authors.initials: S
authors.lastname: Matusra
authors.forename: Constantin C
authors.initials: CC
authors.lastname: Constantinoiu
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsubayashi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tani
authors.forename: Eiichiroh
authors.initials: E
authors.lastname: Baba
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology*; D015153:Blotting, Western / Q000662:veterinary; D002432:Cecum / Q000469:parasitology; D002645:Chickens / Q000469:parasitology*; D003048:Coccidiosis / Q000175:diagnosis / Q000469:parasitology / Q000662:veterinary*; D016786:Eimeria tenella / Q000276:immunology* / Q000648:ultrastructure; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005455:Fluorescent Antibody Technique / Q000662:veterinary; D006825:Hybridomas; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000276:immunology; D007075:Immunoglobulin M / Q000096:biosynthesis / Q000276:immunology; D051379:Mice; D015388:Organelles / Q000737:chemistry / Q000276:immunology; D011201:Poultry Diseases / Q000175:diagnosis / Q000276:immunology / Q000469:parasitology*; D015800:Protozoan Proteins / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D015800:Protozoan Proteins