Expression of conserved mucin domains by epithelial tissues in various mammalian species.
Mucins are related to infectious and non-infectious diseases in Veterinary and Human Medicine. MUC1 mucin is a transmembrane glycoprotein expressed on the apical surface of human epithelia while MUC5AC is the predominant secreted mucin expressed in human gastric epithelium and goblet cells of lung and eyes. MUC5AC C-terminus cysteine rich regions and the cytoplasmic tail of MUC1 domains are conserved among several mammalian species.


OBJECTIVE
to compare the expression of MUC1 and MUC5AC mucins in mammalian epithelia. CT33 anti-MUC1 cytoplasmic tail (MUC1CT) polyclonal antibody and 45M1 anti-MUC5AC monoclonal antibody were employed. By immunohistochemistry, MUC1CT was expressed in most tissues while MUC5AC was restricted to gastric surface epithelium and goblet cells from trachea and lung. By western blot, MUC1CT showed a band at approximately 35 kDa in most tissues; MUC5AC revealed bands at >180 kDa in stomach and lung secretions from rat, cat, pig and cow. When rat MUC5AC was immunoprecipitated, a band at about 180 kDa was obtained.
Lacunza
Ezequiel
E
Centre of Basic and Applied Immunological Research, Faculty of Medical Sciences, National University of La Plata, Calle 60 y 120, 1900 La Plata, Argentina.
Bara
Jacques
J
Segal-Eiras
Amada
A
Croce
María V
MV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000737:chemistry; D015153:Blotting, Western / Q000662:veterinary; D004848:Epithelium / Q000276:immunology; D006801:Humans; D007150:Immunohistochemistry / Q000662:veterinary; D008322:Mammals / Q000276:immunology*; D055271:Mucin 5AC / Q000096:biosynthesis*; D018396:Mucin-1 / Q000096:biosynthesis*; D017434:Protein Structure, Tertiary
D000911:Antibodies, Monoclonal; D055271:Mucin 5AC; D018396:Mucin-1