Prediction of beta-turns and beta-turn types by a novel bidirectional Elman-type recurrent neural network with multiple output layers (MOLEBRNN).
Prediction of beta-turns from amino acid sequences has long been recognized as an important problem in structural bioinformatics due to their frequent occurrence as well as their structural and functional significance. Because various structural features of proteins are intercorrelated, secondary structure information has been often employed as an additional input for machine learning algorithms while predicting beta-turns. Here we present a novel bidirectional Elman-type recurrent neural network with multiple output layers (MOLEBRNN) capable of predicting multiple mutually dependent structural motifs and demonstrate its efficiency in recognizing three aspects of protein structure: beta-turns, beta-turn types, and secondary structure. The advantage of our method compared to other predictors is that it does not require any external input except for sequence profiles because interdependencies between different structural features are taken into account implicitly during the learning process. In a sevenfold cross-validation experiment on a standard test dataset our method exhibits the total prediction accuracy of 77.9% and the Mathew's Correlation Coefficient of 0.45, the highest performance reported so far. It also outperforms other known methods in delineating individual turn types. We demonstrate how simultaneous prediction of multiple targets influences prediction performance on single targets. The MOLEBRNN presented here is a generic method applicable in a variety of research fields where multiple mutually depending target classes need to be predicted.


BACKGROUND
http://webclu.bio.wzw.tum.de/predator-web/.
Kirschner
Andreas
A
Department of Genome Oriented Bioinformatics, Technische Universität München, Weihenstephan, Germany. a.kirschner@wzw.tum.de
Frishman
Dmitrij
D
D000465:Algorithms; D016571:Neural Networks, Computer; D011237:Predictive Value of Tests; D017433:Protein Structure, Secondary; D011506:Proteins / Q000235:genetics*; D020539:Sequence Analysis, Protein / Q000379:methods
D011506:Proteins