title: Crystallization and preliminary X-ray analysis of a class II release factor RF3 from a sulfate-reducing bacterium.
abstract: Class II release factor 3 (RF3) from the sulfate-reducing bacterium Desulfovibrio vulgaris Miyazaki F, which promotes rapid dissociation of a class I release factor, has been overexpressed, purified and crystallized in complex with GDP at 293 K using the sitting-drop vapour-diffusion method. A data set was collected to 1.8 A resolution from a single crystal at 100 K using synchrotron radiation. The crystal belongs to space group P1, with unit-cell parameters a = 47.39, b = 82.80, c = 148.29 A, alpha = 104.21, beta = 89.78, gamma = 89.63 degrees . The asymmetric unit contains four molecules of the RF3-GDP complex. The Matthews coefficient was calculated to be 2.3 A(3) Da(-1) and the solvent content was estimated to be 46.6%.
authors.affiliation: Graduate School of Life Science, University of Hyogo, 3-2-1 Koto, Kamigori-cho, Ako-gun, Hyogo 678-1297, Japan.
authors.forename: Kiyohito
authors.initials: K
authors.lastname: Kihira
authors.forename: Shuko
authors.initials: S
authors.lastname: Numata
authors.forename: Masaya
authors.initials: M
authors.lastname: Kitamura
authors.forename: Jun
authors.initials: J
authors.lastname: Kondo
authors.forename: Shinichi
authors.initials: S
authors.lastname: Terawaki
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Shomura
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Komori
authors.forename: Naoki
authors.initials: N
authors.lastname: Shibata
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Higuchi
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000145:classification* / Q000235:genetics; D003460:Crystallization; D016969:Desulfovibrio vulgaris / Q000737:chemistry* / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D010454:Peptide Termination Factors / Q000737:chemistry* / Q000145:classification* / Q000235:genetics; D013431:Sulfates / Q000737:chemistry* / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D001426:Bacterial Proteins; D010454:Peptide Termination Factors; D013431:Sulfates; C087971:peptide-chain-release factor 3