Rapid screening by real-time 16S rDNA PCR for bacterial contamination of blood products.
Although blood component transfusion is currently regarded as safe, adverse events may occur in recipients of these products. Among those, blood borne viral, bacterial and parasitic infections are best known. For detection of bacterial contamination in platelet concentrates various methods are available or under investigation. One of these methods, real-time polymerase chain reaction (PCR) with particular focus on real-time 16S rDNA detection, will be discussed in this review.
Reesink
Hendrik W
HW
Sanquin Blood Bank North-West Region and Sanquin Diagnostic Services, Amsterdam, The Netherlands. h.reesink@sanquin.nl
Mohammadi
Tamimount
T
Pietersz
Rubyn N I
RN
Savelkoul
Paul H M
PH
D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques; D001792:Blood Platelets / Q000382:microbiology*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004275:DNA, Ribosomal / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D008853:Microscopy; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S