title: Nocardiopsis valliformis sp. nov., an alkaliphilic actinomycete isolated from alkali lake soil in China.
abstract: A strain (HBUM 20028(T)) isolated from alkali lake soil in China was studied by a polyphasic taxonomic approach. The strain produced abundant aerial and substrate mycelia. Long spore chains were borne on the aerial mycelium, and the substrate mycelium was often arranged in a shape like a fence or palisade. The special characteristic of strain HBUM 20028(T) was its abundant growth under alkaline conditions, at pH 8.0-14.0. The cell wall of strain HBUM 20028(T) contained meso-diaminopimelic acid but no diagnostic sugar. Major phospholipids included diphosphatidylglycerol and phosphatidylcholine. The major menaquinones were MK-10(H(2)), MK-10(H(4)) and MK-10(H(6)). The major cellular fatty acids were iso-C(16 : 0) (31.66 %), anteiso-C(17 : 0) (14.85 %) and C(18 : 1)omega9c (14.73 %). All of these characters consistently indicated that strain HBUM 20028(T) belongs to the genus Nocardiopsis. DNA-DNA hybridization between the strain and type strains of related species gave relatedness values far below 70 %. Based on 16S rRNA gene sequence analysis, DNA relatedness and phenotypic characteristics, a novel species with the name Nocardiopsis valliformis sp. nov. is proposed. The type strain is HBUM 20028(T) (=DSM 45023(T) =CGMCC 4.2135(T)).
authors.affiliation: College of Life Sciences, HeBei University, The Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Baoding 071002, PR China.
authors.forename: Runlei
authors.initials: R
authors.lastname: Yang
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Zhang
authors.forename: Li-Ge
authors.initials: LG
authors.lastname: Guo
authors.forename: Nan
authors.initials: N
authors.lastname: Shi
authors.forename: Zhitang
authors.initials: Z
authors.lastname: Lu
authors.forename: Xiumin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000648:ultrastructure; D000468:Alkalies; D002681:China; D005618:Fresh Water; D008855:Microscopy, Electron, Scanning; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013045:Species Specificity
chemical_list: D000468:Alkalies; D012336:RNA, Ribosomal, 16S