[Investigation on antitumor mechanism of polysaccharide from medicinal fungus Penicillium jiangxiense].
OBJECTIVE
To investigate the possible antitumor mechanism of polysaccharide from medicinal fungus Penicillium jiangxiense.


METHODS
The cytotoxic effect was measured by MTT assay, and the cell cycle was analyzed by flow cytometry with Propidium iodide (PI) staining. To apoptotic detections, Hoechst 33258 staining for chromatin, annexin-V FITC/PI double staining for early phase cell apoptosis and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) for late phase cell apoptosis.


RESULTS
Both MPPJ4 and MPPJ5, the fine polysaccharide fractions from P. jiangxiense, showed slight cytotoxic effects to inhibit human gastric adenocarcinoma SGC-7901 cells proliferation, but significantly caused cell cycle arrest at the S phase, and the rate of cell population at the subG1 phase was evidently increased. In apoptotic assays, MPPJ5 was more potent than MMPJ4 in inducing tumor cells in a time-dependent manner at the range from 0 to 72 hours, comparable to the negative control.


CONCLUSIONS
The antitumor acting mechanism of P. jiangxiense polysaccharide is associated with the induction of apoptotic cell death and cell cycle arrest.
Xiao
Jian-hui
JH
Affiliated Hospital of Zunyi Medicinal College, Zunyi 563003, China. jhxiao@yahoo.cn
Fang
Ning
N
Liu
Zu-lin
ZL
Chen
Dai-xiong
DX
D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D032661:Cordyceps / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
D000970:Antineoplastic Agents; D011134:Polysaccharides