IL-18 gene expression pattern in exogenously treated AML cells.
IL-18 production may enhance immune system defense against KG-1 cells ; NB4 cells, which are associated with good prognosis, do not produce IL-18. In this study, we treated KG-1 cells with IL-18 and used microarray technology to assess subsequent effects on gene expression. In UniGene-array of 7488 human genes, expression of 57 genes, including stress related genes, increased at least 2-fold, whereas expression of 48 genes decreased at least 2-fold. Following exogenous exposure of KG-1 cells to IL-18, expression of CRYGC, NF(kappa)BIA and NACA gene were monitored. The latter is a transcriptional coactivator potentiating c-Jun-mediated transcription. NF(kappa)BIA is an inhibitor of NF(kappa)B, and affects growth regulation, apoptosis and hypoxic stress. Studies, such as this one, are beginning to clarify the differences between cells associated with good and bad cancer prognoses, which may ultimately assist in medical treatment for acute myeloid leukemia.
Seo
Minji
M
Department of Biological Science, Sookmyung Woman's University, Seoul, Korea.
Park
Minha
M
Yook
Yeonjoo
Y
Kwon
Young Sook
YS
Suh
Young Ju
YJ
Kim
Min Jung
MJ
Cho
Daeho
D
Park
Jong Hoon
JH
D015153:Blotting, Western; D045744:Cell Line, Tumor; D015536:Down-Regulation; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D006801:Humans; D020382:Interleukin-18 / Q000096:biosynthesis / Q000235:genetics* / Q000494:pharmacology*; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000473:pathology; D016328:NF-kappa B / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D020133:Reverse Transcriptase Polymerase Chain Reaction
D020382:Interleukin-18; D016328:NF-kappa B