[Apoptosis in rat corpus cavernous penis after neruotomy of bilateral dorsal nerve of penis and/or cavernous nerve].
OBJECTIVE
To observe the apoptosis in penile corpus cavernous after neurotomy.


METHODS
After neurotomy, 78 SD rats were randomly assigned to experimental and normal control groups at day 1, 2, 4, 8, 16, and 32 time point. The quality was measured by DNA Ladder and the quantitation was measured by TUNEL. The location of apoptosis was detected by dual stained with immunohistochemistry and TUNEL.


RESULTS
After transaction of cavernous nerve, dorsal never, and both nerves, the apoptosis in experimental and control group showed a statistically significant difference (P<0.0046); however there was no statistically significant difference after bilateral dorsal nerve transaction alone (P>0.0046). There was a statistically significant difference in apoptosis between cavernous smooth muscle cell and endothelial cell groups (P<0.05).


CONCLUSIONS
Cavernous nerve transection induces apoptosis in smooth muscle cells of the rat penis, but nor does dorsal nerve transaction alone. There is no statistically significant difference in apoptosis between cavernous nerve injury alone and combination injuries of cavernous nerve and dorsal nerve.
Ruan
Yi-Sheng
YS
Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R. China. chinafeng24@126.com
Zhu
Guang-You
GY
D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D017763:Autonomic Denervation; D008297:Male; D009130:Muscle, Smooth / Q000473:pathology; D010410:Penile Erection / Q000502:physiology; D010413:Penis / Q000294:innervation* / Q000473:pathology*; D059348:Peripheral Nerve Injuries; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley