title: Determination of phenolic compounds and their antioxidant activity in Erigeron acris L. extracts and pharmaceutical formulation by flow injection analysis with inhibited chemiluminescent detection.
abstract: It was found that the chemiluminescence (CL) produced from the reaction of luminol with iodine in the alkaline medium was strongly inhibited by plant phenolic compounds. Based on this finding, a new flow injection CL method was developed for the determination of caffeic acid and 6'-caffeoylerigeroside. The latter compound was isolated for the first time from Erigeron acris L. herb. The method was simple, rapid and sensitive with a detection limit of 4 x 10(-3) ng mL(-1) (caffeic acid) and 0.18 ng mL(-1) (6'-caffeoylerigeroside), linear range of 0.1-1.5 ng mL(-1) (caffeic acid) and 1-200 ng mL(-1) (6'-caffeoylerigeroside), relative standard deviation of 3.3% for 10 measurements of 0.45 ng mL(-1) caffeic acid and 2.9% for 40 ng mL(-1) 6'-caffeoylerigeroside. This method was successfully applied to determine the content of phenolic compounds/antioxidant activity of E. acris L. extracts and phenolic acids content in pharmaceutical formulation. A possible mechanism of the inhibition of the proposed CL system was discussed.
authors.affiliation: Institute of Chemistry, University of Białystok, ul. Hurtowa 1, 15-399 Białystok, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Nalewajko-Sieliwoniuk
authors.forename: Jolanta
authors.initials: J
authors.lastname: Nazaruk
authors.forename: Ewelina
authors.initials: E
authors.lastname: Antypiuk
authors.forename: Anatol
authors.initials: A
authors.lastname: Kojło
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D002109:Caffeic Acids / Q000494:pharmacology; D049919:Erigeron / Q000737:chemistry*; D017022:Flow Injection Analysis / Q000379:methods; D007455:Iodine / Q000737:chemistry; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation / Q000379:methods*; D008165:Luminol / Q000737:chemistry; D015394:Molecular Structure; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D000975:Antioxidants; D002109:Caffeic Acids; D049408:Luminescent Agents; D010636:Phenols; D010936:Plant Extracts; D008165:Luminol; D007455:Iodine; C040048:caffeic acid