NHS-mediated QDs-peptide/protein conjugation and its application for cell labeling.
3-Mercaptopropyl acid-stabilized CdTe nanoparticles synthesized in aqueous solution are found to be able to conjugate with peptides or proteins mediated by N-hydroxysulfo-succinimide (NHS) but 1-ethyl-3(3-dimethylaminopropyl) carbodiimides hydrochloride (EDC). The reaction time and pH have been optimized. Gel-permeation HPLC was applied following the conjugation, which could quickly and simultaneously detect and purify the quantum dots (QDs) conjugates. The biological activities of QDs conjugates are maintained and give superior results in cell labeling. These results are encouraging regarding the application of QDs molecules for use in living cells, diagnostics and drug delivery.
Shan
Yaming
Y
College of Life Science, Jilin University, Jiefang Road 115, Changchun 130023, PR China.
Wang
Liping
L
Shi
Yuhua
Y
Zhang
Hao
H
Li
Hongmei
H
Liu
Hanzhi
H
Yang
Bai
B
Li
Tianyu
T
Fang
Xuexun
X
Li
Wei
W
D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D000595:Amino Acid Sequence; D000818:Animals; D019187:Cadmium Compounds / Q000737:chemistry; D002417:Cattle; D002460:Cell Line; D002477:Cells / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D009842:Oligopeptides / Q000737:chemistry* / Q000378:metabolism; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism; D045663:Quantum Dots; D012995:Solubility; D013194:Staining and Labeling / Q000379:methods*; D013388:Succinimides / Q000737:chemistry*; D013691:Tellurium / Q000737:chemistry; D013997:Time Factors; D014867:Water / Q000737:chemistry
D019187:Cadmium Compounds; D009842:Oligopeptides; D011506:Proteins; D013388:Succinimides; D014867:Water; C035761:N-hydroxysulfosuccinimide; D015097:3-Mercaptopropionic Acid; D013691:Tellurium; D005022:Ethyldimethylaminopropyl Carbodiimide; C028337:cadmium telluride