Mitochondrial DNA from colorectal cancer cells promotes the malignant phenotype of NIH3T3 cells.
We investigated the potential role of mitochondrial DNA (mtDNA) in colorectal carcinogenesis by constructing a eukaryotic expression vector of the mitochondrial D-loop gene from colorectal cancer cell SW480 and transfected NIH3T3 cells. The NIH3T3/SW480 cells exhibited a significantly increased growth rate and colony formation rate, and also had a decreased apoptotic rate. Polyploidy and aberrant chromosomes were detected in the NIH3T3/SW480 cells by chromosome karyotype analysis. Our results suggested that mtDNA from colorectal cancer cells promotes the malignant phenotype of NIH3T3 cells. Further study of the biological functions of NIH3T3/SW480 cells might be helpful in understanding the role of mtDNA in colorectal carcinogenesis.
Li
Yan
Y
Department of Digestive Diseases, Nanfang Hospital, Southern Medical University, 692 Sheng Li Southern Road, Guangzhou, Guangdong Province 510515, PR China.
Weibing
Song
S
Liu
Hong
H
Hongli
Ji
J
Zhuosheng
Lai
L
Yadong
Wang
W
Bing
Xiao
X
Daiming
Fan
F
D000818:Animals; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002471:Cell Transformation, Neoplastic; D002869:Chromosome Aberrations; D015179:Colorectal Neoplasms / Q000235:genetics*; D004272:DNA, Mitochondrial / Q000378:metabolism*; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D014162:Transfection
D004272:DNA, Mitochondrial