title: An immunisation strategy for the protection of cattle against alcelaphine herpesvirus-1-induced malignant catarrhal fever.
abstract: The aim of this study was to stimulate immunity in the oro-nasal-pharyngeal region of cattle to protect them from alcelaphine herpesvirus-1 (AlHV-1)-induced malignant catarrhal fever. Attenuated C500 strain AlHV-1 was used along with Freund's adjuvant intramuscularly (IM) in the upper neck region to immunise cattle. Virulent C500 strain AlHV-1 was used for intranasal challenge. Nine of ten cattle were protected. Protection was associated with high levels of neutralising antibody in nasal secretions. Some protected animals showed transient low levels of viral DNA in blood samples and in one lymph node sample after challenge whereas viral DNA was detected in the blood and in lymph node samples of all animals with MCF. This is the most promising immunisation strategy to date for the control of malignant catarrhal fever.
authors.affiliation: Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland, UK. david.haig@nottingham.ac.uk
authors.forename: David M
authors.initials: DM
authors.lastname: Haig
authors.forename: Dawn
authors.initials: D
authors.lastname: Grant
authors.forename: David
authors.initials: D
authors.lastname: Deane
authors.forename: Iris
authors.initials: I
authors.lastname: Campbell
authors.forename: Jackie
authors.initials: J
authors.lastname: Thomson
authors.forename: Catherine
authors.initials: C
authors.lastname: Jepson
authors.forename: David
authors.initials: D
authors.lastname: Buxton
authors.forename: George C
authors.initials: GC
authors.lastname: Russell
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis; D002417:Cattle; D004279:DNA, Viral / Q000097:blood; D005620:Freund's Adjuvant / Q000008:administration & dosage; D018142:Gammaherpesvirinae / Q000276:immunology*; D007273:Injections, Intramuscular; D008198:Lymph Nodes / Q000821:virology; D008297:Male; D008304:Malignant Catarrh / Q000517:prevention & control*; D009061:Mouth Mucosa / Q000276:immunology; D009297:Nasal Mucosa / Q000276:immunology; D009500:Neutralization Tests; D010614:Pharynx / Q000276:immunology; D016019:Survival Analysis; D014613:Vaccines, Attenuated / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage / Q000276:immunology*; D014766:Viremia
chemical_list: D000276:Adjuvants, Immunologic; D000914:Antibodies, Viral; D004279:DNA, Viral; D014613:Vaccines, Attenuated; D014765:Viral Vaccines; D005620:Freund's Adjuvant