title: Determination of dimethylarginine levels in rats using HILIC-MS/MS: an in vivo microdialysis study.
abstract: Nitric oxide (NO) is one of the most important mediators and neurotransmitters and its levels change under pathological conditions. NO production may be regulated by endogenous nitric oxide synthase (NOS) inhibitors, in particular asymmetric dimethylarginine (ADMA). Most of the interest is focused on ADMA, since this compound is present in plasma and urine and accumulation of ADMA has been described in many disease states but little is known about cerebrospinal fluid (CSF) concentrations of this compound and of its structural isomer symmetric dimethylarginine (SDMA). To determine the levels of methylarginines, we here present a new hydrophilic interaction chromatography (HILIC)-MS/MS method for the precise determination of these substances in CSF from microdialysis samples of rat prefrontal cortex (PFC). The method requires only minimal sample preparation and features isotope-labelled internal standards.
authors.affiliation: Department of Biomedical Sciences, University of Foggia, Foggia, Italy.
authors.forename: Margherita
authors.initials: M
authors.lastname: Zotti
authors.forename: Stefania
authors.initials: S
authors.lastname: Schiavone
authors.forename: Filomena
authors.initials: F
authors.lastname: Tricarico
authors.forename: Marilena
authors.initials: M
authors.lastname: Colaianna
authors.forename: Oceania
authors.initials: O
authors.lastname: D'Apolito
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Paglia
authors.forename: Gaetano
authors.initials: G
authors.lastname: Corso
authors.forename: Luigia
authors.initials: L
authors.lastname: Trabace
mesh_terms: D000818:Animals; D001120:Arginine / Q000031:analogs & derivatives* / Q000134:cerebrospinal fluid / Q000592:standards; D002853:Chromatography, Liquid / Q000379:methods*; D004791:Enzyme Inhibitors / Q000134:cerebrospinal fluid / Q000592:standards; D017551:Microdialysis / Q000379:methods*; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D017397:Prefrontal Cortex / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012015:Reference Standards; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D004791:Enzyme Inhibitors; C024917:symmetric dimethylarginine; C018524:N,N-dimethylarginine; D001120:Arginine; D019001:Nitric Oxide Synthase