title: Caloric restriction retards the age-related decline in mitochondrial function of brown adipose tissue.
abstract: Caloric restriction (CR) has been shown to prevent the age-associated loss of mitochondrial function and biogenesis in several tissues such as liver, heart, and skeletal muscle. However, little is known about the effects of CR on a tissue in which the mitochondria have no adenosine triphosphate (ATP)-producing purpose but show a high degree of uncoupling, namely brown adipose tissue (BAT). Hence, the aim of the present study was to analyze the effect of long-term CR on BAT mitochondrial function and biogenesis. BAT mitochondria obtained from 24-month-old male and female rats previously subjected to 40% CR for 12 months were compared with mitochondria from old (24 months) and young (6 months) ad libitum fed rats. Old restricted rats compared to old ad libitum fed ones showed a reduction in BAT size with respect to fat content and adipocyte number. Mitochondrial DNA content in BAT increased with age and even more so in restricted rats, indicating a summative effect of age and CR on mitochondrial proliferation. CR induced resistance to lose total and mitochondrial protein, COX activity, and uncoupling capacity with advancing age, in relation with a lower decrease of mitochondrial transcription factor A (TFAM). In summary, our results demonstrate CR prevents the age-associated decline in mitochondrial function in BAT, probably in relation with a lower impairment of mitochondrial biogenesis.
authors.affiliation: Grup de Metabolisme Energètic i Nutrició, Departament de Biologia Fonamental i Ciències de la Salut, Universitat de les Illes Balears, Palma de Mallorca, Ciber Fisiopatología Obesidad y Nutrición (CB06/03) Instituto Salud Carlos III, Spain.
authors.forename: Adamo
authors.initials: A
authors.lastname: Valle
authors.forename: Rocío
authors.initials: R
authors.lastname: Guevara
authors.forename: Francisco José
authors.initials: FJ
authors.lastname: García-Palmer
authors.forename: Pilar
authors.initials: P
authors.lastname: Roca
authors.forename: Jordi
authors.initials: J
authors.lastname: Oliver
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D002001:Adipose Tissue, Brown / Q000378:metabolism*; D000375:Aging / Q000378:metabolism*; D000818:Animals; D001833:Body Temperature Regulation; D031204:Caloric Restriction; D004268:DNA-Binding Proteins / Q000032:analysis / Q000502:physiology; D005260:Female; D007473:Ion Channels / Q000032:analysis / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D024101:Mitochondrial Proteins / Q000032:analysis / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D014157:Transcription Factors / Q000032:analysis / Q000502:physiology; D000071256:Uncoupling Protein 1
chemical_list: D004268:DNA-Binding Proteins; D007473:Ion Channels; D024101:Mitochondrial Proteins; C499631:TFAM protein, human; D014157:Transcription Factors; D000071256:Uncoupling Protein 1; D000255:Adenosine Triphosphate