title: Prevalence of HIV-associated cryptococcal meningitis and utility of microbiological determinants for its diagnosis in a tertiary care center.
abstract: BACKGROUND
  Human immunodeficiency virus (HIV) infection continues to be the most important risk factor for the development of central nervous system (CNS) cryptococcosis, which in turn is an important contributor to morbidity and mortality in HIV-infected patients. Early diagnosis of such patients is the key to their therapeutic success.
  
  
  OBJECTIVE
  This study was undertaken to find out the prevalence of CNS cryptococcosis and to assess the role of microbiological parameters for its specific diagnosis in HIV-reactive hospitalized patients admitted with meningeal signs in a tertiary care setting.
  
  
  METHODS
  A total of 104 patients suspected to be suffering from meningitis/meningoencephalitis were subjected to cerebrospinal fluid (CSF) analysis (including India ink preparation, culture by conventional methods and Bactec MGIT 960 system, antigen detection) and tests for HIV antibodies by standard laboratory operating procedures.
  
  
  RESULTS
  The prevalence of HIV infection in our study group was 12.5% (13/104), while the prevalence of cryptococcal CNS infection in HIV-reactive cohort was 46% (6/13). Additionally, 15.3% (2/13) of the patients from this cohort were positive for Mycobacterium tuberculosis.
  
  
  CONCLUSIONS
  High prevalence of cryptococcal CNS infections in HIV-infected patients underscores the importance of precise and early microbiological diagnosis for better management of such patients.
authors.affiliation: Department of Microbiology, IHBAS, Delhi,, India. drajeevthakur@rediffmail.com
authors.forename: Rajeev
authors.initials: R
authors.lastname: Thakur
authors.forename: Smita
authors.initials: S
authors.lastname: Sarma
authors.forename: Suman
authors.initials: S
authors.lastname: Kushwaha
mesh_terms: D017088:AIDS-Related Opportunistic Infections / Q000175:diagnosis* / Q000453:epidemiology; D000293:Adolescent; D000328:Adult; D000368:Aged; D002648:Child; D005260:Female; D015528:HIV Seroprevalence; D006801:Humans; D007194:India / Q000453:epidemiology; D008297:Male; D016919:Meningitis, Cryptococcal / Q000150:complications / Q000175:diagnosis* / Q000453:epidemiology; D008875:Middle Aged