title: [Evaluation of neutrophile elastase and isoprostane 8epiPGF2alpha concentrations in maternal and umbilical cord blood serum and in amniotic fluid in pregnancies complicated by premature rupture of membranes].
abstract: OBJECTIVE
  To evaluate the total isoprostane 8-epi-PGF2alpha and neutrophil elastase (NE) concentrations in pregnancies complicated by premature rupture of membranes (PROM).
  
  
  METHODS
  128 pregnant women were divided into four groups: pregnancies complicated by PROM between 24.-36.(PPBP-N) and between 38 a 41 weeks of gestation (PPBP-D), uncomplicated pregnancies between 24-36 gestation weeks (K1) and pregnancies delivered by cesarean section (before uterine contractions had started) after 38 weeks (K2). The concentrations of NE and isoprostane 8-epi-PGF2alpha were measured in maternal serum, cord blood serum and in the amniotic fluid.
  
  
  RESULTS
  The following study revealed higher concentrations of NE in maternal serum and in the amniotic fluid than in the umbilical cord blood in PROM cases, and lower amniotic fluid than maternal serum concentrations in the control groups. Also, the levels of isoprostane differentiated between compartments in particular groups. In both groups complicated with PROM, higher maternal serum and amniotic fluid NE concentrations than in controls were found. There were no differences in isoprostane levels between the groups.
  
  
  CONCLUSIONS
  1. Higher concentrations of NE in maternal blood serum and in the amniotic fluid than in the umbilical cord blood in PROM cases, as well as lower amniotic fluid than maternal serum concentrations in the controls, may be connected with pathogenesis of PROM. 2. Differentiated maternal serum, cord serum and amniotic fluid isoprostane concentrations may suggest various intensity of oxidative stress in particular compartments. 3. Lack of differences in maternal serum, cord serum and amniotic fluid isoprostane concentrations may suggest similar intensity of oxidative stress in cases with PROM and intact membranes.
authors.affiliation: Klinika Połoznictwa i Ginekologii Katedry Połoznictwa, Ginekologii i Neonatologii Pomorskiej Akademii Medycznej w Szczecinie. sebak@sci.pam.szczecin.pl
authors.forename: Sebastian
authors.initials: S
authors.lastname: Kwiatkowski
authors.forename: Ryszard
authors.initials: R
authors.lastname: Czajka
authors.forename: Barbara
authors.initials: B
authors.lastname: Dołegowska
authors.forename: Dariusz
authors.initials: D
authors.lastname: Chlubek
authors.forename: Andrzej
authors.initials: A
authors.lastname: Torbé
mesh_terms: D000328:Adult; D000653:Amniotic Fluid / Q000378:metabolism*; D016022:Case-Control Studies; D015237:Dinoprost / Q000031:analogs & derivatives* / Q000032:analysis; D005260:Female; D005312:Fetal Blood / Q000378:metabolism*; D005322:Premature Rupture of Fetal Membranes / Q000378:metabolism*; D006801:Humans; D019272:Leukocyte Elastase / Q000032:analysis*; D007752:Obstetric Labor, Premature / Q000378:metabolism; D018384:Oxidative Stress; D011044:Poland; D011247:Pregnancy; D012307:Risk Factors
chemical_list: C075750:8-epi-prostaglandin F2alpha; D015237:Dinoprost; D019272:Leukocyte Elastase