title: Short- and long-term alterations of mitochondrial morphology, dynamics and mtDNA after transient oxidative stress.
abstract: Cells are exposed during their life span to fluctuating levels of reactive oxygen species (ROS). To investigate the effects of a single ROS boost in vitro, human endothelial cells (HUVEC) were treated with one short-term dose of hydrogen peroxide. This treatment resulted in a short, dose-dependent ROS peak that caused transient changes in the mitochondrial morphology and fine structure, in the frequency of mitochondrial fission and fusion and in the mRNA levels of distinct fission and fusion factors. Treatment with a higher dose induced prolonged mtDNA damage; these cells exhibited a significantly shortened replicative lifespan, indicating dose-dependent effects of oxidative stress on mitochondria.
authors.affiliation: Kinematic Cell Research Group, Institute for Cell Biology and Neuroscience, Center of Excellence Frankfurt, Macromolecular Complexes, JW Goethe University, Max-von-Laue-Str. 9, 60438 Frankfurt/Main, Germany. jendrach@bio.uni-frankfurt.de
authors.forename: Marina
authors.initials: M
authors.lastname: Jendrach
authors.forename: Sören
authors.initials: S
authors.lastname: Mai
authors.forename: Sandra
authors.initials: S
authors.lastname: Pohl
authors.forename: Monika
authors.initials: M
authors.lastname: Vöth
authors.forename: Jürgen
authors.initials: J
authors.lastname: Bereiter-Hahn
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D004272:DNA, Mitochondrial / Q000187:drug effects / Q000235:genetics*; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000187:drug effects / Q000502:physiology / Q000648:ultrastructure*; D018384:Oxidative Stress / Q000235:genetics*; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D006861:Hydrogen Peroxide