title: A chalcone synthase-like gene is highly expressed in the tapetum of both wheat (Triticum aestivum L.) and triticale (xTriticosecale Wittmack).
abstract: A novel anther-specific chalcone synthase-like gene, TaCHSL1, was isolated and characterized. The TaCHSL1 transcript was detected only within the tapetum during the "free" and early vacuolated microspore stages in both wheat and triticale. Sequence analysis indicated that the 41.8 kDa TaCHSL1 deduced protein belongs to a small distinct group of type III polyketide synthases that includes angiosperm and gymnosperm orthologs shown to be anther-specific. TaCHSL1 sequence characteristics and conservation, as well as its restricted expression pattern, point to a distinct and important biochemical role in developing anthers.
authors.affiliation: Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, KW Neatby Bldg, 960 Carling Ave., Ottawa, ON, K1A 0C6, Canada.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Wu
authors.forename: Stephen J B
authors.initials: SJ
authors.lastname: O'Leary
authors.forename: Steve
authors.initials: S
authors.lastname: Gleddie
authors.forename: François
authors.initials: F
authors.lastname: Eudes
authors.forename: André
authors.initials: A
authors.lastname: Laroche
authors.forename: Laurian S
authors.initials: LS
authors.lastname: Robert
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D002523:Edible Grain / Q000235:genetics*; D035264:Flowers / Q000201:enzymology / Q000235:genetics*; D017343:Genes, Plant; D008969:Molecular Sequence Data; D018749:RNA, Plant / Q000235:genetics; D016415:Sequence Alignment; D014908:Triticum / Q000201:enzymology / Q000235:genetics*
chemical_list: D018076:DNA, Complementary; D018749:RNA, Plant; D000217:Acyltransferases; C020141:flavanone synthetase