title: Lipid peroxidation-related 1,N2-propanodeoxyguanosine-DNA adducts induced by endogenously formed 4-hydroxy-2-nonenal in organs of female rats fed diets supplemented with sunflower, rapeseed, olive or coconut oil.
abstract: Animal and epidemiological studies confirm an impact of the fatty-acid composition in the diet on cancer development. We investigated the role of supplementation of the diet of female F344-rats with sunflower, rapeseed, olive or coconut oil on the formation of the promutagenic, exocyclic 1,N2-propanodeoxyguanosine adduct of the main lipid peroxidation product 4-hydroxy-2-nonenal in the mucosa of the glandular stomach, the small intestine, the colon, the whole kidney and the lung. This adduct is considered as the predominant DNA adduct arising from lipid peroxidation. The correlations between adduct levels and the different fatty acids were not uniform for all organs. No clear relationships between fatty acids and adduct levels were found in the colon. Significant positive correlations were observed between linoleic acid, total polyunsaturated fatty acids (PUFAs), vitamin E and DNA adduct levels in the small intestine and in the kidney. The results indicate an increasing effect on cancer risk in these organs as a result of high intake of linoleic acid. Inverse relationships between linoleic acid, PUFA and vitamin E intake and adduct levels were found in the glandular stomach and the lung. We could not confirm a chemopreventive effect of linolenic acid (C-18 omega-3 PUFA) on the formation of adducts in our animal study, as was shown in white blood cells of women in a previous study. A tendency towards a decrease in adduct levels was seen with monounsaturated fatty acids (MUFAs) in all organs except the lung. Saturated fatty acids showed a significant positive correlation with adduct levels in the mucosa of the glandular stomach and a significant inverse correlation in the small intestine. Saturated fatty acids are not considered to directly influence lipid peroxidation to a major extent.
authors.affiliation: Department of Toxicology, University of Würzburg, 97078 Würzburg, Germany. eder@toxi.uni-wuerzburg.de
authors.forename: Erwin
authors.initials: E
authors.lastname: Eder
authors.forename: Matthias
authors.initials: M
authors.lastname: Wacker
authors.forename: Paul
authors.initials: P
authors.lastname: Wanek
mesh_terms: D000447:Aldehydes / Q000378:metabolism*; D000818:Animals; D029741:Brassica rapa / Q000737:chemistry; D000074263:Coconut Oil; D003106:Colon / Q000378:metabolism; D018736:DNA Adducts; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000378:metabolism; D005260:Female; D005753:Gastric Mucosa / Q000378:metabolism; D006368:Helianthus / Q000737:chemistry; D007421:Intestine, Small / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D019787:Linoleic Acid / Q000494:pharmacology; D015227:Lipid Peroxidation; D008168:Lung / Q000378:metabolism; D000069463:Olive Oil; D010938:Plant Oils / Q000494:pharmacology*; D051381:Rats; D011916:Rats, Inbred F344; D014810:Vitamin E
chemical_list: C090855:1,N(2)-(1,3-propano)-2'-deoxyguanosine; D000447:Aldehydes; D018736:DNA Adducts; D000069463:Olive Oil; D010938:Plant Oils; D014810:Vitamin E; D019787:Linoleic Acid; D003849:Deoxyguanosine; C027576:4-hydroxy-2-nonenal; D000074263:Coconut Oil