title: Metabolic analysis of four phenolic acids in rat by liquid chromatography-tandem mass spectrometry.
abstract: A liquid chromatography-diode array detection-electrospray ionization ion trap mass spectrometry (LC-DAD-ESI-MS(n)) method was established for the analysis of danshensu, caffeic acid, ferulic acid and isoferulic acid in rat plasma, bile, urine and feces after oral administration or intravenous injection. Liquid-liquid extraction was employed for the preparation of biosamples, and the chromatographic separation was carried out using an Agilent Zorbax Extend C(18) reversed phase column and acetonitrile-0.1% formic acid as the mobile phase. Totally nineteen metabolites were detected and identified as prototype, methylated, hydroxylated, sulfated and glucuronized conjugates. The metabolism of the individual phenolic acids in biosamples was investigated, and the metabolic pathway was proposed. By comparing the metabolism of different compounds which shared similar structures, we were able to find that methylation was the main pathway of danshensu metabolism, and the double bond on the side chain was critical for the drug excretion via bile and the formation of glucuronized conjugates. The results proved that the established method was simple, sensitive and reliable, which could be used to detect and identify the structures of metabolites and to better understand their in vivo metabolism.
authors.affiliation: The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, China.
authors.forename: Zi-Chuan
authors.initials: ZC
authors.lastname: Zhang
authors.forename: Man
authors.initials: M
authors.lastname: Xu
authors.forename: Shi-Feng
authors.initials: SF
authors.lastname: Sun
authors.forename: Xue
authors.initials: X
authors.lastname: Qiao
authors.forename: Bao-Rong
authors.initials: BR
authors.lastname: Wang
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
mesh_terms: D000818:Animals; D002109:Caffeic Acids / Q000032:analysis* / Q000378:metabolism; D002853:Chromatography, Liquid / Q000379:methods*; D002934:Cinnamates / Q000032:analysis* / Q000378:metabolism; D003373:Coumaric Acids / Q000032:analysis* / Q000378:metabolism; D005243:Feces / Q000737:chemistry; D007773:Lactates / Q000032:analysis* / Q000378:metabolism; D053858:Metabolic Networks and Pathways; D010636:Phenols / Q000097:blood / Q000378:metabolism* / Q000652:urine; D051381:Rats; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D002109:Caffeic Acids; D002934:Cinnamates; D003373:Coumaric Acids; D007773:Lactates; D010636:Phenols; C035055:3,4-dihydroxyphenyllactic acid; C004999:ferulic acid; C040048:caffeic acid; C008180:isoferulic acid