Bovine serum albumin adsorption on nano-rough platinum surfaces studied by QCM-D.
The adsorption of bovine serum albumin (BSA) on platinum surfaces with a root-mean-square roughness ranging from 1.49nm to 4.62nm was investigated using quartz crystal microbalance with dissipation (QCM-D). Two different BSA concentrations, 50microg/ml and 1mg/ml, were used, and the adsorption studies were complemented by monitoring the antibody interaction with the adsorbed BSA layer. The adsorption process was significantly influenced by the surface nano-roughness, and it was observed that the surface mass density of the adsorbed BSA layer is enhanced in a non-trivial way with the surface roughness. From a close examination of the energy dissipation vs. frequency shift plot obtained by the QCM-D technique, it was additionally observed that the BSA adsorption on the roughest surface is subject to several distinct adsorption phases revealing the presence of structural changes facilitated by the nano-rough surface morphology during the adsorption process. These changes were in particular noticeable for the adsorption at the low (50microg/ml) BSA concentration. The results confirm that the nano-rough surface morphology has a significant influence on both the BSA mass uptake and the functionality of the resulting protein layer.
Dolatshahi-Pirouz
A
A
Department of Physics and Astronomy, University of Aarhus, DK-8000 Aarhus C, Denmark.
Rechendorff
K
K
Hovgaard
M B
MB
Foss
M
M
Chevallier
J
J
Besenbacher
F
F
D000327:Adsorption; D000818:Animals; D002417:Cattle; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D007074:Immunoglobulin G / Q000378:metabolism; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D010984:Platinum / Q000737:chemistry* / Q000378:metabolism*; D011791:Quartz / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism*; D013499:Surface Properties
D020099:Coated Materials, Biocompatible; D007074:Immunoglobulin G; D011791:Quartz; D012710:Serum Albumin, Bovine; D010984:Platinum