title: RNA extraction from polysaccharide-based cell-laden hydrogel scaffolds.
abstract: An effective method for obtaining high-quality RNA from polysaccharide-rich hydrogels would be of great interest to biomaterialists and tissue engineers. Based on the similarities between polysaccharide-based hydrogels and plant tissues, we used a plant-specific RNA extraction kit to extract RNA from mammalian cells encapsulated in hydrogel scaffolds. The results indicate that this method can be reliably used in isolating high-purity RNA from polysaccharide hydrogels.
authors.affiliation: Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, N1.3-B2-13, Singapore 637457, Republic of Singapore.
authors.forename: Chunming
authors.initials: C
authors.lastname: Wang
authors.forename: Jinghua
authors.initials: J
authors.lastname: Hao
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.forename: Kai
authors.initials: K
authors.lastname: Su
authors.forename: Dong-An
authors.initials: DA
authors.lastname: Wang
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000737:chemistry / Q000166:cytology; D011134:Polysaccharides / Q000737:chemistry*; D012313:RNA / Q000302:isolation & purification*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001672:Biocompatible Materials; D020100:Hydrogels; D011134:Polysaccharides; D012313:RNA