Male specific expression of lipocalin-type prostaglandin D synthase (cPTGDS) during chicken gonadal differentiation: relationship with cSOX9.
As in mammals, the SOX9 gene (cSOX9) is specifically expressed in male differentiating Sertoli cells during chicken gonadal development. Recent studies in mouse have shown that the prostaglandin D(2) (PGD(2))/lipocalin-type prostaglandin D synthase (PTGDS) pathway is functionally associated with the regulation of SOX9 and is specifically expressed in male developing gonads. In this study, we have shown that, as in mammals, cPTGDS is a male specific gene during chicken testicular development and is temporally expressed in the same window as cSOX9 in Sertoli cells. Using a culture of gonadal explants, we have shown that exogenous PGD(2) enhances cSOX9 expression in male, and activates its ectopic expression in female gonads without up-regulating cAMH. These data indicate a conserved PGD(2) modulation of SOX9 expression during testicular differentiation between birds and mammals.
Moniot
B
B
Institute of Human Genetics, CNRS UPR1142, Montpellier, France.
Boizet-Bonhoure
B
B
Poulat
F
F
D000818:Animals; D002645:Chickens / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D006066:Gonads / Q000166:cytology / Q000187:drug effects / Q000201:enzymology*; D019746:Intramolecular Oxidoreductases / Q000235:genetics / Q000378:metabolism*; D054834:Lipocalins / Q000235:genetics / Q000378:metabolism*; D008297:Male; D015230:Prostaglandin D2 / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D055758:SOX9 Transcription Factor / Q000235:genetics*; D012727:Sex Characteristics; D012733:Sex Differentiation / Q000187:drug effects; D013997:Time Factors; D014158:Transcription, Genetic / Q000187:drug effects
D054834:Lipocalins; D012333:RNA, Messenger; D055758:SOX9 Transcription Factor; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase; D015230:Prostaglandin D2