Anti-mullerian hormone and inhibin B in the definition of ovarian aging and the menopause transition.
OBJECTIVE
The objective of the study was to determine whether anti-Mullerian hormone (AMH) and inhibin B are viable endocrine biomarkers for framing the menopause transition from initiation to the final menstrual period (FMP).


METHODS
We assayed AMH, inhibin B, and FSH in 300 archival follicular phase specimens from 50 women with six consecutive annual visits commencing in 1993 when all women were in the pre- and perimenopausal menopause stages. Subsequently each woman had a documented FMP. The assay results were fitted as individual-woman profiles and then related to time to FMP and age at FMP as outcomes.


RESULTS
Based on annual values from six time points prior to the FMP, (log)AMH longitudinal profiles declined and were highly associated with a time point 5 yr prior to FMP [including both observed and values below detection (P < 0.0001 and P = 0.0001, respectively)]. Baseline AMH profiles were also associated with age at FMP (P = 0.035). Models of declining (log)inhibin B profiles (including both observed and values below detection) were associated with time to FMP (P < 0.0001 and P = 0.0003, respectively). There was no significant association of (log)inhibin B profiles with age at FMP.


CONCLUSIONS
AMH, an endocrine marker that reflects the transition of resting primordial follicles to growing follicles, declined to a time point 5 yr prior to the FMP; this may represent a critical biological juncture in the menopause transition. Low and nondetectable levels inhibin B levels also were observed 4-5 yr prior to the FMP but were less predictive of time to FMP or age at FMP.
Sowers
MaryFran R
MR
Department of Epidemiology, School of Public Health, University of Michigan, 109 Observatory, Room 1846, Ann Arbor, Michigan 48109, USA. mfsowers@umich.edu
Eyvazzadeh
Aimee D
AD
McConnell
Daniel
D
Yosef
Matheos
M
Jannausch
Mary L
ML
Zhang
Daowen
D
Harlow
Sioban
S
Randolph
John F
JF
D000328:Adult; D000375:Aging / Q000097:blood* / Q000502:physiology; D054304:Anti-Mullerian Hormone / Q000097:blood / Q000502:physiology*; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D005260:Female; D005640:Follicle Stimulating Hormone / Q000097:blood; D005544:Forecasting; D006801:Humans; D007265:Inhibins / Q000097:blood / Q000502:physiology*; D008593:Menopause / Q000097:blood* / Q000502:physiology; D008598:Menstruation / Q000097:blood; D008875:Middle Aged; D008954:Models, Biological; D010053:Ovary / Q000502:physiology*
D015415:Biomarkers; C422364:inhibin B; D007265:Inhibins; D054304:Anti-Mullerian Hormone; D005640:Follicle Stimulating Hormone