title: [Analysis of appropriate amount of template DNA for sinofiler kit by real time quantitative PCR technique].
abstract: OBJECTIVE
  To explore the appropriate amount of template DNA for Sinofiler Kit.
  
  
  METHODS
  The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay.
  
  
  RESULTS
  It was shown that 1.29-1.51 ng of template DNA in 12.5 microL reaction volume was optimal for STR genotyping with Sinofiler Kit.
  
  
  CONCLUSIONS
  Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.
authors.affiliation: Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R. China. lichengtaohla@163.com
authors.forename: Cheng-Tao
authors.initials: CT
authors.lastname: Li
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
authors.forename: Yuan
authors.initials: Y
authors.lastname: Lin
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.forename: Ting-Zhi
authors.initials: TZ
authors.lastname: Que
authors.forename: Li
authors.initials: L
authors.lastname: Li
mesh_terms: D004247:DNA / Q000032:analysis*; D005554:Forensic Medicine / Q000379:methods*; D006197:Hair / Q000737:chemistry; D006801:Humans; D018895:Microsatellite Repeats; D011933:Reagent Kits, Diagnostic; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D013698:Templates, Genetic
chemical_list: D011933:Reagent Kits, Diagnostic; D004247:DNA