title: [Investigation on antitumor mechanism of polysaccharide from medicinal fungus Penicillium jiangxiense].
abstract: OBJECTIVE
  To investigate the possible antitumor mechanism of polysaccharide from medicinal fungus Penicillium jiangxiense.
  
  
  METHODS
  The cytotoxic effect was measured by MTT assay, and the cell cycle was analyzed by flow cytometry with Propidium iodide (PI) staining. To apoptotic detections, Hoechst 33258 staining for chromatin, annexin-V FITC/PI double staining for early phase cell apoptosis and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) for late phase cell apoptosis.
  
  
  RESULTS
  Both MPPJ4 and MPPJ5, the fine polysaccharide fractions from P. jiangxiense, showed slight cytotoxic effects to inhibit human gastric adenocarcinoma SGC-7901 cells proliferation, but significantly caused cell cycle arrest at the S phase, and the rate of cell population at the subG1 phase was evidently increased. In apoptotic assays, MPPJ5 was more potent than MMPJ4 in inducing tumor cells in a time-dependent manner at the range from 0 to 72 hours, comparable to the negative control.
  
  
  CONCLUSIONS
  The antitumor acting mechanism of P. jiangxiense polysaccharide is associated with the induction of apoptotic cell death and cell cycle arrest.
authors.affiliation: Affiliated Hospital of Zunyi Medicinal College, Zunyi 563003, China. jhxiao@yahoo.cn
authors.forename: Jian-hui
authors.initials: JH
authors.lastname: Xiao
authors.forename: Ning
authors.initials: N
authors.lastname: Fang
authors.forename: Zu-lin
authors.initials: ZL
authors.lastname: Liu
authors.forename: Dai-xiong
authors.initials: DX
authors.lastname: Chen
mesh_terms: D000970:Antineoplastic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D032661:Cordyceps / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides