Immunogenicity and safety of a novel formalin-inactivated and alum-adjuvanted candidate subunit vaccine for mumps.
While an attenuated vaccine against mumps has played a role in controlling the epidemic of this disease worldwide, some problems with efficacy and safety of the vaccine are still present. In the work described here, a novel mumps vaccine with good immunity and safety was developed by selecting an antigen component of the mumps virus. The results suggest that this purified antigen vaccine is immunogenic in animals and is capable of inducing a specific neutralizing antibody response against viral HN, but not against other viral proteins. The clinical and pathological observations after challenge of vaccinated rhesus monkeys indicated further that the immune response induced by this vaccine provided complete protection from wild-type virus infection. Furthermore, the immunological analysis and clinical observation of experimental monkeys that were immunized with the vaccine, followed by infection with wild-type virus, suggest that no abnormal changes in expression of inflammatory cytokines and no clinical allergic reaction were found at 1 month after challenge.
Liang
Yan
Y
Institute of Medical Biology, Chinese Academy of Medicine Science, Peking Union Medical College, 379# Jiaoling Road, Kunming 650118 PR China.
Ma
Shaohui
S
Yang
Zijiang
Z
Liu
Longding
L
Wang
Lichun
L
Wang
Jingjing
J
Jiang
Li
L
Shi
Changjun
C
Dong
Chenghong
C
Li
Qihan
Q
D000276:Adjuvants, Immunologic / Q000008:administration & dosage / Q000494:pharmacology; D000534:Alum Compounds / Q000008:administration & dosage / Q000494:pharmacology; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D016207:Cytokines / Q000276:immunology; D004202:Disinfectants / Q000494:pharmacology; D005260:Female; D005557:Formaldehyde / Q000494:pharmacology; D015707:HN Protein / Q000276:immunology; D006967:Hypersensitivity; D008253:Macaca mulatta; D008297:Male; D009107:Mumps / Q000517:prevention & control; D009108:Mumps Vaccine / Q000009:adverse effects* / Q000276:immunology*; D009109:Mumps virus / Q000187:drug effects; D009500:Neutralization Tests; D010306:Parotid Gland / Q000473:pathology; D022223:Vaccines, Subunit / Q000009:adverse effects / Q000276:immunology; D038301:Virus Inactivation
D000276:Adjuvants, Immunologic; D000534:Alum Compounds; D000914:Antibodies, Viral; D016207:Cytokines; D004202:Disinfectants; D015707:HN Protein; D009108:Mumps Vaccine; D022223:Vaccines, Subunit; D005557:Formaldehyde; C041524:aluminum sulfate