title: CatSper genes expression, semen characteristics and histology of testes in the contusive spinal cord-injured mice model.
abstract: METHODS
  Experimental study.
  
  
  OBJECTIVE
  A recently characterized CatSper genes, encodes for unique Ca(2+) channels in the testes, where they play essential roles in sperm motility. The aim of this research is to evaluate potential changes in the expression of CatSper genes, sperm parameters and testis histology following spinal cord injury (SCI).
  
  
  METHODS
  Department of Anatomical Sciences, Tarbiat Modares University, Tehran, Iran.
  
  
  METHODS
  A total of 75 adult NMRI mice were divided into three groups (25 in each group) of SCI, sham and control. Following laminectomy, SCI group was subjected to injury at the ninth thoracic vertebra. The epididymal sperm parameters were studied at day 1 and at weeks 1, 2, 4 and 6 after injury. One testis was removed for morphological study and the other testis were used for molecular study. Reverse transcription-PCR was performed at different time in all the groups.
  
  
  RESULTS
  Our results revealed that SCI affects spermatogenesis as well as sperm quality and quantity. In gene expression analysis, there was a significant downregulation of CatSpers 1 and 2 at 4 and 6 weeks following contusion. There were no differences between the semen parameters and CatSper genes expression at the different time points in the sham and control groups.
  
  
  CONCLUSIONS
  Our data indicated that the SCI mouse model causes a significant reduction in all sperm parameters, along with deleterious effects on spermatogenesis as well as the expression of CatSpers 1 and 2.
authors.affiliation: Department of Anatomical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: J
authors.initials: J
authors.lastname: Rezaian
authors.forename: M
authors.initials: M
authors.lastname: Movahedin
authors.forename: S J
authors.initials: SJ
authors.lastname: Mowla
mesh_terms: D000818:Animals; D015220:Calcium Channels / Q000235:genetics*; D004195:Disease Models, Animal; D015536:Down-Regulation / Q000235:genetics; D020869:Gene Expression Profiling; D007473:Ion Channels / Q000235:genetics; D007796:Laminectomy / Q000379:methods; D008297:Male; D051379:Mice; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012661:Semen / Q000378:metabolism*; D029589:Seminal Plasma Proteins / Q000235:genetics; D013081:Sperm Motility / Q000502:physiology; D013091:Spermatogenesis / Q000502:physiology; D013119:Spinal Cord Injuries / Q000503:physiopathology*; D013737:Testis / Q000378:metabolism* / Q000473:pathology; D013997:Time Factors
chemical_list: C522969:CATSPER3 protein, mouse; C522970:CATSPER4 protein, mouse; D015220:Calcium Channels; C480636:CatSper2 protein, mouse; C488642:Catsper1 protein, mouse; D007473:Ion Channels; D012333:RNA, Messenger; D029589:Seminal Plasma Proteins