title: Study on the retention equation in hydrophilic interaction liquid chromatography.
abstract: Hydrophilic interaction liquid chromatography (HILIC) is an effective technique for separating polar compounds. But its retention equation has not been studied systematically yet. In this study, an appropriate retention equation was established by using eight nucleosides as model analytes and by comparing four retention models on six different HILIC columns. As a result, retention equation Ink = a + b x InC(B) + cxC(B) could be quantitatively described the retention factors with good accuracy in HILIC mode. Based on this equation, the retention times of eight nucleosides under the conditions by other mobile phase can be predicted on each column. All of the predicted relative errors of retention times were smaller than 5%. The established retention model was also successfully applied to predict retention times of the real Traditional Chinese Medicine-Carthaus tinctorius L. sample.
authors.affiliation: Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
authors.forename: Gaowa
authors.initials: G
authors.lastname: Jin
authors.forename: Zhimou
authors.initials: Z
authors.lastname: Guo
authors.forename: Feifang
authors.initials: F
authors.lastname: Zhang
authors.forename: Xingya
authors.initials: X
authors.lastname: Xue
authors.forename: Yu
authors.initials: Y
authors.lastname: Jin
authors.forename: Xinmiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification; D008956:Models, Chemical; D009705:Nucleosides / Q000302:isolation & purification; D055672:Static Electricity
chemical_list: D004365:Drugs, Chinese Herbal; D009705:Nucleosides