Nanostructured porous silicon as functionalized material for biosensor application.
In this work by means of PL, FTIR and XPS techniques, state-of-the-art porous silicon (PS) films with good mechanical and optical properties have been effectively utilized for the biofunctionalization purpose for its possible application in immunosensors. The functionalization of the PS surface has been achieved by silanization process using aminopropyltriethoxysilane (APTS) as a precursor. The presence of reactive amino groups on the PS surface along with glutaraldehyde as a linker aids in the covalent binding of the antibody (Human IgG) onto the PS surface. Different antigen concentrations can be detected with a good reproducibility with this technique which opens a huge possibility of using this biofunctionalized material for future biosensors.
Singh
Shalini
S
Electronic Materials Division, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India.
Sharma
Shailesh N
SN
Govind
Shivaprasad
S M
SM
Lal
Mohan
M
Khan
Mukhtar A
MA
D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods; D007074:Immunoglobulin G / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity; D011485:Protein Binding; D012825:Silicon / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D013499:Surface Properties
D055910:Antibodies, Immobilized; D007074:Immunoglobulin G; D012825:Silicon