Rat prostate tumors express cancer procoagulant, an activator of coagulation factor X.
Two common procoagulant activities associated with tumors are tissue factor and cancer procoagulant (CP), an activator of coagulation factor X. We have identified a convenient source of CP in transplanted Lobund-Wistar rat PA3 prostate tumors. CP activity was purified from 5 independent transplanted prostate tumors by column chromatography. The protein activated factor X in the absence of TF and factor VII. An antihuman CP antibody recognized rat CP in an ELISA and inactivated CP activity in a chromogenic assay. Lobund-Wistar prostate tumors may provide a convenient animal model useful in determining the role of CP in cancer development.
Kamocka
Malgorzata
M
Medical University of Lodz, Lodz, Poland. mkamocka@iupui.edu
Pollard
Morris
M
Suckow
Mark
M
Mielicki
Wojciech P
WP
Rosen
Elliot D
ED
D000818:Animals; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004797:Enzyme-Linked Immunosorbent Assay; D005170:Factor X / Q000378:metabolism*; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar
D009363:Neoplasm Proteins; D005170:Factor X; D003546:Cysteine Endopeptidases; C030910:cancer procoagulant