title: Heavy metals extraction from municipal solid waste incineration fly ash using adapted metal tolerant Aspergillus niger.
abstract: This study focused on the adaptation of Aspergillus niger tolerating high concentration of heavy metals for bioleaching of fly ash. The Plackett-Burman design indicated that Al and Fe inhibited the growth of A. niger (AS 3.879 and AS 3.40) significantly. The single metal (Al and Fe) and multi-metals adapted AS 3.879 strain tolerated up to 3500 mg/L Al, 700 mg/L Fe, and 3208.1mg/L multi-metals, respectively. The order of metal extraction yield in two-step bioleaching of 60 and 70 g/L fly ash using Al adapted, multi-metals adapted and un-adapted AS 3.879 strains was as follows: multi-metals adapted>Al adapted>un-adapted. The multi-metals adapted strain grew with up to 70 g/L fly ash and secreted 256 mmol/L organic acids after 288 h, where 87.4% Cd, 64.8% Mn, 49.4% Zn and 45.9% Pb were dissolved. The extracted metals in TCLP test of the bioleached fly ash by multi-metals adapted strain were under the regulated levels in China.
authors.affiliation: Shanghai Academy of Environmental Sciences, Shanghai, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Yang
authors.forename: Qunhui
authors.initials: Q
authors.lastname: Wang
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.forename: Tingji
authors.initials: T
authors.lastname: Wu
mesh_terms: D001234:Aspergillus niger / Q000145:classification* / Q000378:metabolism*; D002244:Carbon / Q000302:isolation & purification / Q000378:metabolism*; D002947:Cities; D060729:Coal Ash; D004361:Drug Tolerance; D019216:Metals, Heavy / Q000302:isolation & purification / Q000378:metabolism*; D052638:Particulate Matter / Q000302:isolation & purification / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods*; D013045:Species Specificity
chemical_list: D060729:Coal Ash; D019216:Metals, Heavy; D052638:Particulate Matter; D002244:Carbon