title: Association of platelet-derived growth factor-D gene polymorphism with ischemic stroke in a Chinese case-control study.
abstract: Stroke is a multiple genetic disease. Platelet-derived growth factor-D has been found to be involved in the pathogenesis of atherosclerosis, suggesting possible association between platelet-derived growth factor-D and the development of ischemic stroke. However, little information on the relationship between platelet-derived growth factor-D and stroke is currently available. The aim of this study was to investigate the association between platelet-derived growth factor-D genetic variation and the risk of ischemic stroke in a Chinese population. We conducted a case-control study with 309 ischemic stroke patients and 309 sex and age (<5 years)-matched controls. DNA was extracted from the whole blood of each participant. Platelet-derived growth factor-D C/G polymorphism at position +3166 (rs7950273) was detected by TaqMan SNP genotyping assay. Overall, the combined rates of platelet-derived growth factor- D CG and GG are 51% in patients in contrast with 46% in controls. There were no significant differences in the genotype frequencies of platelet-derived growth factor-D +3166 polymorphisms between the patients and controls with history or family history of hypertension or diabetes (P = 0.770). However, among people without history or family history of hypertension or diabetes, platelet-derived growth factor-D CG/GG is significantly more frequently expressed in patients (60%) than in controls (43%) (odds ratio 1.97; 95% confidence interval 1.19-3.26). This significant association holds after adjustment for age, sex, smoking and alcohol intaking (odds ratio 1.86; 95% confidence interval 1.11-3.10) (P = 0.018). Our study found that the G allele of rs7950273 of the platelet-derived growth factor-D gene is associated with higher risk of ischemic stroke in a Chinese population without history or family history of hypertension or diabetes. Future studies with larger and ethnically diverse populations are needed to further evaluate the platelet-derived growth factor-D polymorphism and stroke association, as well as its pathophysiological mechanisms.
authors.affiliation: Department of Epidemiology and Statistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, People's Republic of China.
authors.forename: Sheng-Hong
authors.initials: SH
authors.lastname: Han
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Liu
authors.forename: Alai
authors.initials: A
authors.lastname: Tan
authors.forename: Ji
authors.initials: J
authors.lastname: Peng
authors.forename: Ren-Li
authors.initials: RL
authors.lastname: Zhang
authors.forename: Shi-Tong
authors.initials: ST
authors.lastname: Gao
authors.forename: Da-Na
authors.initials: DN
authors.lastname: Huang
authors.forename: Lei
authors.initials: L
authors.lastname: Yu
authors.forename: Chong-Jian
authors.initials: CJ
authors.lastname: Wang
authors.forename: Jin-Quan
authors.initials: JQ
authors.lastname: Cheng
authors.forename: Shao-Fa
authors.initials: SF
authors.lastname: Nie
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D044466:Asian People; D002545:Brain Ischemia / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D003920:Diabetes Mellitus / Q000097:blood / Q000235:genetics; D005260:Female; D030342:Genetic Diseases, Inborn / Q000097:blood / Q000235:genetics*; D006801:Humans; D006973:Hypertension / Q000097:blood / Q000235:genetics; D008222:Lymphokines / Q000097:blood / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010982:Platelet-Derived Growth Factor / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D012307:Risk Factors; D020521:Stroke / Q000097:blood / Q000235:genetics*
chemical_list: D008222:Lymphokines; C427493:PDGFD protein, human; D010982:Platelet-Derived Growth Factor