Components of the gene network associated with genotype-dependent response of wheat to the Fusarium mycotoxin deoxynivalenol.
The Fusarium mycotoxin deoxynivalenol (DON) facilitates fungal spread within wheat tissue and the development of Fusarium head blight disease. The ability of wheat spikelets to resist DON-induced bleaching is genotype-dependent. In wheat cultivar (cv.) CM82036 DON resistance is associated with a quantitative trait locus, Fhb1, located on the short arm of chromosome 3B. Gene expression profiling (microarray and real-time RT-PCR analyses) of DON-treated spikelets of progeny derived from a cross between cv. CM82036 and the DON-susceptible cv. Remus discriminated ten toxin-responsive transcripts associated with the inheritance of DON resistance and Fhb1. These genes do not exclusively map to Fhb1. Based on the putative function of the ten Fhb1-associated transcripts, we discuss how cascades involving classical metabolite biotransformation and sequestration processes, alleviation of oxidative stress and promotion of cell survival might contribute to the host response and defence against DON.
Walter
Stephanie
S
Molecular Plant-Microbe Interactions Laboratory, School of Biology and Environmental Sciences, College of Life Sciences, University College Dublin, Belfield, Dublin 4, Ireland. stephanie.walter@ucd.ie
Brennan
Josephine M
JM
Arunachalam
Chanemougasoundharam
C
Ansari
Khairul I
KI
Hu
Xuejun
X
Khan
Mojibur R
MR
Trognitz
Friederike
F
Trognitz
Bodo
B
Leonard
Gerald
G
Egan
Damian
D
Doohan
Fiona M
FM
D005670:Fusarium / Q000378:metabolism / Q000472:pathogenicity; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D053263:Gene Regulatory Networks; D005838:Genotype; D008969:Molecular Sequence Data; D009183:Mycotoxins / Q000378:metabolism*; D020411:Oligonucleotide Array Sequence Analysis; D014255:Trichothecenes / Q000378:metabolism*; D014908:Triticum / Q000235:genetics / Q000382:microbiology
D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol