[Protective effects of Guangdong Liangcha grandes on restraint stress-induced liver damage in mice].
OBJECTIVE
To investigate the effect of Guangdong Liangcha Keli on restraint stress-induced liver damage in mice.


METHODS
Thirty-five male C57BL/6J mice of 7 weeks old were divided into 5 groups randomly with 7 mice in each group: normal group, restraint stress group, 250 mg kg(-1) Vitamin C, Guangdong Liangcha Keli 500 mg kg(-1) and 250 mg kg(-1). After 18 hr restraint stress, the ALT acitivity in plasma, MDA level in plasma and liver, GSH content, GSH-PX and GST activities, NO level and ORAC value in liver were determined.


RESULTS
Compared with restraint model group, Guangdong Liangcha Keli could markedly reduce ALT activity (92.75 +/- 1.91 vs 39.29 +/- 2.56, 32.69 +/- 1.46) U L(-1), and protect the liver damage induced by oxidative stress. In addition, Guangdong Liangcha Keli could effectively increase the ORAC value, GSH content, GSH-PX activity and GST activity and reduce the MDA level and NO level in liver.


CONCLUSIONS
Oral treatment of Guangdong Liangcha Keli is found to reduce restraint stress-induced liver damage in terms of above mentioned biochemical parameters, and these protective effects may be related to its free radical scavenging activity and lipid peroxidation inhibitory effects.
Bao
Li
L
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
Yao
Xin-Sheng
XS
He
Rong-Rong
RR
Kurihara
Hiroshi
H
D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000494:pharmacology*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008107:Liver Diseases / Q000097:blood / Q000209:etiology* / Q000378:metabolism / Q000517:prevention & control*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009569:Nitric Oxide / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000150:complications*
D004365:Drugs, Chinese Herbal; C540315:Guangdong Liangcha; D009569:Nitric Oxide; D000410:Alanine Transaminase; D005978:Glutathione