Myeloid differentiation factor 88-silenced bone marrow-derived dendritic cells exhibit enhanced tolerogenicity in intestinal transplantation in rats.
Myeloid differentiation factor 88 (MyD88) is a key adaptor of Toll-like receptor signaling, which plays a critical role in dendritic cell (DC) function. However, its role in the induction of transplant tolerance by immature DCs is unknown. In this study, we silenced MyD88 expression of bone marrow-derived immature DCs with small interference RNA demonstrating that this maneuver significantly prolonged the survival of intestinal allografts. This study provided evidence that the absence of MyD88 enhances the tolerogenicity of DCs and suggested that inhibiting innate immunity may be a potential strategy to facilitate induction of transplant tolerance by DCs.
Zhu
C
C
Department of Gastrointestinal Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Xu
H
H
Zhang
G
G
Lu
C
C
Ji
M
M
Wu
W
W
D000818:Animals; D001854:Bone Marrow Cells / Q000276:immunology*; D016026:Bone Marrow Transplantation / Q000276:immunology*; D003713:Dendritic Cells / Q000276:immunology*; D020868:Gene Silencing; D007108:Immune Tolerance; D007422:Intestines / Q000637:transplantation*; D008297:Male; D053594:Myeloid Differentiation Factor 88 / Q000172:deficiency* / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D017207:Rats, Sprague-Dawley; D014162:Transfection; D014184:Transplantation, Homologous
D053594:Myeloid Differentiation Factor 88; D034741:RNA, Small Interfering