title: GabaB receptors activation in the NTS blocks the glycemic responses induced by carotid body receptor stimulation.
abstract: The carotid body receptors participate in glucose regulation sensing glucose levels in blood entering the cephalic circulation. The carotid body receptors information, is initially processed within the nucleus tractus solitarius (NTS) and elicits changes in circulating glucose and brain glucose uptake. Previous work has shown that gamma-aminobutyric acid (GABA) in NTS modulates respiratory reflexes, but the role of GABA within NTS in glucose regulation remains unknown. Here we show that GABA(B) receptor agonist (baclofen) or antagonists (phaclofen and CGP55845A) locally injected into NTS modified arterial glucose levels and brain glucose retention. Control injections outside NTS did not elicit these responses. In contrast, GABA(A) agonist and antagonist (muscimol or bicuculline) produced no significant changes in blood glucose levels. When these GABAergic drugs were applied before carotid body receptors stimulation, again, only GABA(B) agonist or antagonist significantly affected glycemic responses; baclofen microinjection significantly reduced the hyperglycemic response and brain glucose retention observed after carotid body receptors stimulation, while phaclofen produced the opposite effect, increasing significantly hyperglycemia and brain glucose retention. These results indicate that activation of GABA(B), but not GABA(A), receptors in the NTS modulates the glycemic responses after anoxic stimulation of the carotid body receptors, and suggest the presence of a tonic inhibitory mechanism in the NTS to avoid hyperglycemia.
authors.affiliation: CUIB, Universidad de Colima, Colima, Col. 28045, México.
authors.forename: Mónica
authors.initials: M
authors.lastname: Lemus
authors.affiliation: CUIB, Universidad de Colima, Colima, Col. 28045, México; Facultad de Medicina, Universidad de Colima, Colima, Col. 28045, Mexico.
authors.forename: Sergio
authors.initials: S
authors.lastname: Montero
authors.affiliation: CUIB, Universidad de Colima, Colima, Col. 28045, México.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Cadenas
authors.affiliation: CUIB, Universidad de Colima, Colima, Col. 28045, México.
authors.forename: José Jesús
authors.initials: JJ
authors.lastname: Lara
authors.affiliation: CUIB, Universidad de Colima, Colima, Col. 28045, México.
authors.forename: Héctor Rafael
authors.initials: HR
authors.lastname: Tejeda-Chávez
authors.affiliation: CUIB, Universidad de Colima, Colima, Col. 28045, México.
authors.forename: Ramón
authors.initials: R
authors.lastname: Álvarez-Buylla
authors.affiliation: CUIB, Universidad de Colima, Colima, Col. 28045, México. Electronic address: rab@ucol.mx.
authors.forename: Elena Roces
authors.initials: ER
authors.lastname: de Álvarez-Buylla
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001418:Baclofen / Q000008:administration & dosage / Q000031:analogs & derivatives / Q000494:pharmacology; D001640:Bicuculline / Q000008:administration & dosage / Q000494:pharmacology; D001786:Blood Glucose / Q000032:analysis; D002344:Carotid Body / Q000187:drug effects* / Q000502:physiology; D018755:GABA Agonists / Q000008:administration & dosage / Q000494:pharmacology; D018756:GABA Antagonists / Q000008:administration & dosage / Q000494:pharmacology; D005947:Glucose / Q000378:metabolism*; D008845:Microinjections; D009118:Muscimol / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D011963:Receptors, GABA-A / Q000502:physiology; D018080:Receptors, GABA-B / Q000502:physiology*; D017552:Solitary Nucleus / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology
chemical_list: D001786:Blood Glucose; D018755:GABA Agonists; D018756:GABA Antagonists; D011963:Receptors, GABA-A; D018080:Receptors, GABA-B; C052329:phaclofen; D009118:Muscimol; D001418:Baclofen; D005947:Glucose; D001640:Bicuculline