[A method designed for the assessment of myocardial metabolism in rats with 18F-FDG using small-animal-PET. Initial experience in Mexico].
OBJECTIVE
To standarize an acquisition protocol for the study of myocardial metabolism in adult rats.


METHODS
Three Wistar adult male rats were studied in three different protocols: no fasting group, fasting group over a period of 12 hr before the study with only water provided ad libitum, and fasting group by the same time receiving an oral 50% glucose solution. Thirty-minute acquisition images were obtained with a micro-PET, thirty and sixty minutes after the administration of 370-555 MBq 18F-FDG. Comparative and visual analysis were performed by two experts in the field.


RESULTS
Eigtheen studies were analyzed, six per group. The best images were those of the first group, especially those taken at 60 minutes after the 18F-FDG administration.


CONCLUSIONS
It is possible to establish the non-fasting protocol for the assessment of myocardial metabolism to be used in the future for the myocardial viability evaluation in ischemic cardiopathy.
Rosas
Erick Alexánderson
EA
Universidad Nacional Autónoma de México (UNAM), Facultad de Medicina, Ciudad Universitaria, México, D.F. 04510. alexanderick@yahoo.com.mx
López
Nayelli Ortega
NO
Flores
Rafael Ojeda
RO
Vázquez
Guillermo Mendoza
GM
Ocampo
Gloria Adame
GA
González
Aloha Meave
AM
Domínguez
Yolanda Saldaña
YS
Molina
Pedro A Lamothe
PA
Valero
Mónica Rodriguez
MR
Castillo
Leonardo García Rojas
LG
Robles
José Narro
JN
D000818:Animals; D019788:Fluorodeoxyglucose F18; D006321:Heart / Q000000981:diagnostic imaging*; D008297:Male; D008800:Mexico; D009206:Myocardium / Q000378:metabolism*; D049268:Positron-Emission Tomography; D019275:Radiopharmaceuticals; D051381:Rats; D017208:Rats, Wistar
D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18