title: Loss of neuronal projections in the dystrophin-deficient mdx mouse is not progressive.
abstract: Lack of dystrophin is known to reduce several cerebral fiber systems. To investigate if the loss of fibers is progressive, we analyzed projections of the trigeminal sensory system to the red nucleus in 3, 6, and 12 month old dystrophin-deficient mdx mice. The retrograde tracer fluorogold was injected in the magnocellular part of the red nucleus, and the number of labeled neurons in the oral part of the spinal trigeminal nucleus (Sp5O) was counted. We found that the number of labeled Sp5O neurons was reduced by 50% in mdx mice compared to age-matched control mice. The number of labeled Sp5O neurons did not change significantly between 3 and 12 months neither in mdx nor in control mice. In addition, the number of labeled neurons in the interstitial system of the trigeminal nerve was reduced by 43% in mdx mice. We conclude that fiber loss did not continue beyond the age of 3 months. Our data suggest that lack of full-length dystrophin impairs neuronal migration or axonal outgrowth, or increases neuronal death during fetal or early life.
authors.affiliation: Department of Histology, Universidade Federal de São Paulo, São Paulo, Brazil.
authors.forename: Magali Luci
authors.initials: ML
authors.lastname: Pinto
authors.forename: Heloisa Helena Vieira Olyntho
authors.initials: HH
authors.lastname: Tokunaga
authors.forename: Caden
authors.initials: C
authors.lastname: Souccar
authors.forename: Guus H M
authors.initials: GH
authors.lastname: Schoorlemmer
authors.forename: Rita de Cássia Ribeiro
authors.initials: Rde C
authors.lastname: da Silva Lapa
mesh_terms: D000818:Animals; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D001931:Brain Mapping; D002452:Cell Count; D016923:Cell Death / Q000235:genetics; D004195:Disease Models, Animal; D016189:Dystrophin / Q000235:genetics*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D009410:Nerve Degeneration / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D009421:Nervous System Malformations / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology; D009434:Neural Pathways / Q000002:abnormalities / Q000166:cytology / Q000378:metabolism; D012012:Red Nucleus / Q000002:abnormalities* / Q000166:cytology / Q000378:metabolism; D013266:Stilbamidines; D014276:Trigeminal Nerve / Q000002:abnormalities / Q000166:cytology / Q000378:metabolism; D014279:Trigeminal Nucleus, Spinal / Q000002:abnormalities* / Q000166:cytology / Q000378:metabolism; D014855:Wallerian Degeneration / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology
chemical_list: C049774:2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; D016189:Dystrophin; D013266:Stilbamidines