title: Ultrasonic-assisted enzymatic digestion (USAED) for total elemental determination and elemental speciation: a tutorial.
abstract: Due to its potential as sample treatment for Analytical Chemistry, the Ultrasonic-Assisted Enzymatic Digestion (USAED) for total elemental determination and elemental speciation is described under the most recent achievements published in literature, focusing on the variables that critically affect the performance of this relatively new sample treatment, such as the type of enzymes or the type of ultrasonic system used for the acceleration of the solid-liquid extraction process. Moreover, analytical chemists are aware of common errors produced in data interpretation concerning USAED. In addition, a guide for the rapid application of this methodology is also provided along with detailed explanations. Finally, future trends regarding USAED are also given and commented.
authors.affiliation: REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Monte da Caparica, Portugal.
authors.forename: G
authors.initials: G
authors.lastname: Vale
authors.forename: R
authors.initials: R
authors.lastname: Rial-Otero
authors.forename: A
authors.initials: A
authors.lastname: Mota
authors.forename: L
authors.initials: L
authors.lastname: Fonseca
authors.forename: J L
authors.initials: JL
authors.lastname: Capelo
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D019064:Product Packaging; D013696:Temperature; D014465:Ultrasonics
chemical_list: D004798:Enzymes