Reaction properties of hydrophobic enzymes and their immobilization on phospholipid composite membranes.
Reaction rates of hydrophobic enzymes, aminopeptidase, and alkaline phosphatase, in microsomes prepared from the porcine brush border membrane and in vesicles pre pared from microsomes and phospholipids were measured at various temperatures. Interactions between the hydrophobic enzymes and the phospholipid layers are discussed as well as the effects of fluidity change of phospholipid layers on enzyme activity. Further, reaction properties and stabilities of the immobilized vesicles containing microsomal enzymes were studied.
Sada
E
E
Chemical Engineering Department, Kyoto University, Kyoto 606, Japan.
Katoh
S
S
Terashima
M
M
Kikuchi
Y
Y