title: A live attenuated cold-adapted influenza A H7N3 virus vaccine provides protection against homologous and heterologous H7 viruses in mice and ferrets.
abstract: The appearance of human infections caused by avian influenza A H7 subtype viruses underscores their pandemic potential and the need to develop vaccines to protect humans from viruses of this subtype. A live attenuated H7N3 virus vaccine was generated by reverse genetics using the HA and NA genes of a low pathogenicity A/chicken/BC/CN-6/04 (H7N3) virus and the six internal protein genes of the cold-adapted A/Ann Arbor/6/60 ca (H2N2) virus. The reassortant H7N3 BC 04 ca vaccine virus was temperature sensitive and showed attenuation in mice and ferrets. Intranasal immunization with one dose of the vaccine protected mice and ferrets when challenged with homologous and heterologous H7 viruses. The reassortant H7N3 BC 04 ca vaccine virus showed comparable levels of attenuation, immunogenicity and efficacy in mice and ferret models. The safety, immunogenicity, and efficacy of this vaccine in mice and ferrets support the evaluation of this vaccine in clinical trials.
authors.affiliation: Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA.
authors.forename: Tomy
authors.initials: T
authors.lastname: Joseph
authors.forename: Josephine
authors.initials: J
authors.lastname: McAuliffe
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
authors.forename: Leatrice
authors.initials: L
authors.lastname: Vogel
authors.forename: David
authors.initials: D
authors.lastname: Swayne
authors.forename: Hong
authors.initials: H
authors.lastname: Jin
authors.forename: George
authors.initials: G
authors.lastname: Kemble
authors.forename: Kanta
authors.initials: K
authors.lastname: Subbarao
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002642:Chick Embryo; D002645:Chickens / Q000821:virology; D003080:Cold Temperature; D005289:Ferrets / Q000276:immunology / Q000821:virology*; D053121:Influenza A Virus, H2N2 Subtype / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D009980:Influenza A virus / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000472:pathogenicity; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D005585:Influenza in Birds / Q000276:immunology / Q000517:prevention & control / Q000821:virology; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000401:mortality / Q000517:prevention & control* / Q000821:virology; D010641:Phenotype; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology* / Q000472:pathogenicity; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000276:immunology*; D014779:Virus Replication
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated