title: [Dynamic changes of LDH and HBDH activity in rabbit serum after low voltage electrical injury].
abstract: OBJECTIVE
  To investigate changes of LDH and HBDH activity in rabbit serum after non-thermal low voltage electrical injury and to provide diagnostic criteria for non-thermal low voltage electrical injury.
  
  
  METHODS
  Forty New Zealand rabbits were randomly distributed into control group and electrical injury group (EI-groups; designated 7 time points: 0 h, 2 h, 4 h, 12 h, 1 d, 2 d, 3 d), 5 rabbits per each group. EI-groups were treated with the method of non-thermal low voltage electrical injury established in our laboratory. Ventricular blood (5 mL) was obtained under anesthesia at designated time points after electrical injury. The activities of LDH and HBDH were measured.
  
  
  RESULTS
  Dynamic changes were observed with certain patterns from target serum enzyme activities after electrical injury. Compared with control group, the activities of LDH increased markedly at 4 h, 12 h, and on days 1, 2, and 3 after injury (4 h, 12 h, and day 1 P<0.01; day 2 and day 3 P<0.05). Activities of HBDH increased markedly at day 2 and day 3 after injury (P<0.05). The ratio of HBDH/LDH decreased markedly at 2 h, 4 h, and 12 h after injury (P<0.01).
  
  
  CONCLUSIONS
  Dynamic changes of LDH and HBDH activities may be useful in diagnosis of non-thermal low voltage electrical injury and in estimation of post injury intervals.
authors.affiliation: Second Hospital of Shijiazhuang, Shijiazhuang 050051, China. xuyunqiang9@sina.com
authors.forename: Yun-Qiang
authors.initials: YQ
authors.lastname: Xu
authors.forename: Yan-Kui
authors.initials: YK
authors.lastname: Jin
authors.forename: Jun-Qing
authors.initials: JQ
authors.lastname: Ren
authors.forename: Min
authors.initials: M
authors.lastname: Zuo
authors.forename: Song-Jun
authors.initials: SJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D004556:Electric Injuries / Q000201:enzymology*; D005260:Female; D049429:Forensic Pathology; D006884:Hydroxybutyrate Dehydrogenase / Q000097:blood*; D007770:L-Lactate Dehydrogenase / Q000097:blood*; D008297:Male; D011817:Rabbits; D011897:Random Allocation
chemical_list: D007770:L-Lactate Dehydrogenase; D006884:Hydroxybutyrate Dehydrogenase