Identification of five phospho-beta-glycosidases from Lactobacillus gasseri ATCC33323T cultured in lactose medium.
Lactobacillus gasseri ATCC33323(T) has seven putative phospho-beta-glycosidase genes. Using column chromatography, we found that this strain cultured in lactose medium expresses five phospho-beta-glycosidases (LacG1, LacG2, Pbg1, Pbg2, and Pbg3), where these gene expressions can be suppressed by glucose. To our knowledge, this is the first report indicating that five glycosidases are induced from a single bacterial strain using a single carbon source, lactose.
Nagaoka
Seiji
S
Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi, Japan.
Honda
Hiroyuki
H
Ohshima
Satoru
S
Kawai
Yasushi
Y
Kitazawa
Haruki
H
Tateno
Yoshio
Y
Yamazaki
Yukiko
Y
Saito
Tadao
T
D002845:Chromatography; D015870:Gene Expression / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology; D007778:Lactobacillus / Q000201:enzymology* / Q000378:metabolism; D007785:Lactose / Q000494:pharmacology*; D008828:Microbiological Techniques; D010750:Phosphoproteins / Q000302:isolation & purification; D001616:beta-Galactosidase / Q000302:isolation & purification*
D010750:Phosphoproteins; D001616:beta-Galactosidase; D005947:Glucose; D007785:Lactose