title: Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts.
abstract: The effects of laser phototherapy on the release of growth factors by human gingival fibroblasts were studied in vitro. Cells from a primary culture were irradiated twice (6 h interval), with continuous diode laser [gallium-aluminum-arsenium (GaAlAs), 780 nm, or indium-gallium-aluminum-phosphide (InGaAlP),_660 nm] in punctual and contact mode, 40 mW, spot size 0.042 cm(2), 3 J/cm(2) and 5 J/cm(2) (3 s and 5 s, respectively). Positive [10% fetal bovine serum (FBS)] and negative (1%FBS) controls were not irradiated. Production of keratinocyte growth factor (KGF) and basic fibroblast growth factor (bFGF) was quantified by enzyme-linked immunosorbent assay (ELISA). The data were statistically compared by analysis of variance (ANOVA) followed by Tukey's test (P </= 0.05). The characterization of the cell line indicated a mesenchymal nature. KGF release was similar in all groups, while that of bFGF was significantly greater (1.49-times) in groups treated with infra-red laser. It was concluded that increased production of bFGF could be one of the mechanisms by which infra-red laser stimulates wound healing.
authors.affiliation: Departamento de Periodontia, Faculdade de Odontologia, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Carla Andreotti
authors.initials: CA
authors.lastname: Damante
authors.forename: Giorgio
authors.initials: G
authors.lastname: De Micheli
authors.forename: Sueli Patrícia Harumi
authors.initials: SP
authors.lastname: Miyagi
authors.forename: Ilíria Salomão
authors.initials: IS
authors.lastname: Feist
authors.forename: Márcia Martins
authors.initials: MM
authors.lastname: Marques
mesh_terms: D000818:Animals; D002417:Cattle; D002478:Cells, Cultured; D003470:Culture Media; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis; D051523:Fibroblast Growth Factor 7 / Q000096:biosynthesis; D005346:Fibroblast Growth Factors / Q000096:biosynthesis*; D005347:Fibroblasts / Q000378:metabolism / Q000528:radiation effects; D005881:Gingiva / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects*; D005893:Gingivoplasty; D006801:Humans; D028022:Low-Level Light Therapy; D010789:Phototherapy; D014945:Wound Healing / Q000502:physiology / Q000528:radiation effects
chemical_list: D003470:Culture Media; C496453:FGF7 protein, human; D016222:Fibroblast Growth Factor 2; D051523:Fibroblast Growth Factor 7; D005346:Fibroblast Growth Factors