Relation between menin expression and NF-kappaB activity in an intestinal cell line.
In a previous study, we demonstrated that the Men1 gene is mainly expressed in the proliferative crypt compartment of the small intestine and that a reduction of menin expression in the crypt-like IEC-17 cell line induces an increase in proliferation rate concomitant with an increase in cyclin D1 expression. The aim of the present study was to test the hypothesis that the NF-kappaB pathway may be involved in cyclin D1 overexpression. Transcriptional activity of the cyclin D1 gene promoter was increased upon reduction of menin expression. Blockade of the NF-kappaB pathway restored proliferation, cell cycle, cyclin D1 gene transcription and cyclin D1 expression levels to those observed in the presence of menin. These data support a correlation between cyclin D1 expression, NF-kappaB activity and menin expression in this epithelial cell line and are relevant to the physiological function of menin in regulating proliferation in the intestinal epithelium.
Theillaumas
Aurélie
A
Inserm, U865, IFR Lyon Est, Faculté Laënnec, 69372 Lyon Cedex 8, France.
Blanc
Martine
M
Couderc
Christophe
C
Poncet
Gilles
G
Bazzi
Wissam
W
Bernard
Christine
C
Cordier-Bussat
Martine
M
Scoazec
Jean Yves
JY
Roche
Colette
C
D000818:Animals; D002453:Cell Cycle / Q000502:physiology; D002460:Cell Line; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D004386:Duodenum / Q000166:cytology / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D007413:Intestinal Mucosa / Q000166:cytology*; D011401:Promoter Regions, Genetic; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D051996:Transcription Factor RelA / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
C105373:MEN1 protein, human; C532041:Men1 protein, rat; D011518:Proto-Oncogene Proteins; D051996:Transcription Factor RelA; D014157:Transcription Factors; D019938:Cyclin D1