title: Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study.
abstract: We describe how phase-modulation laser interference microscopy and wavelet analysis can be applied to noninvasive nonstained visualization and study of the structural and dynamical properties of living cells. We show how phase images of erythrocytes can reveal the difference between various erythrocyte forms and stages of hemolysis and how phase images of neurons reveal their complex intracellular structure. Temporal variations of the refractive index are analyzed to detect cellular rhythmic activity on different time scales as well as to uncover interactions between the cellular processes.
authors.affiliation: Technical University of Denmark, Department of Physics, 2800 Kongens Lyngby, Denmark. brazhe@fysik.dtu.dk
authors.forename: Alexey R
authors.initials: AR
authors.lastname: Brazhe
authors.forename: Nadezda A
authors.initials: NA
authors.lastname: Brazhe
authors.forename: Georgy V
authors.initials: GV
authors.lastname: Maksimov
authors.forename: Pavel S
authors.initials: PS
authors.lastname: Ignatyev
authors.forename: Andrey B
authors.initials: AB
authors.lastname: Rubin
authors.forename: Erik
authors.initials: E
authors.lastname: Mosekilde
authors.forename: Olga V
authors.initials: OV
authors.lastname: Sosnovtseva
mesh_terms: D002478:Cells, Cultured; D004912:Erythrocytes / Q000166:cytology*; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D007368:Interferometry / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods*; D008858:Microscopy, Phase-Contrast / Q000379:methods*; D041623:Tomography, Optical Coherence / Q000379:methods*