Identification of gangliosides recognized by IgG anti-GalNAc-GD1a antibodies in bovine spinal motor neurons and motor nerves.
The presence of immunoglobulin G (IgG)-type antibodies to the ganglioside, N-acetylgalactosaminyl GD1a (GalNAc-GD1a), is closely associated with the pure motor type of Guillain-Barré syndrome (GBS). In the present study, we isolated disialogangliosides from the motor neurons and motor nerves of bovine spinal cords by DEAE-Sephadex column chromatography. The disialoganglioside fraction contained GD1a, GD2, GD1b, and three gangliosides, designated X1, X2 and X3. Serum from a patient with axonal GBS with IgG anti-GalNAc-GD1a antibody yielded positive immunostaining with X1, X2, and X3. When isolated by preparative thin-layer chromatography (TLC), X1 migrated at the same position as GalNAc-GD1a from Tay-Sachs brain, suggesting that X1 is GalNAc-GD1a containing N-acetylneuraminic acid (NeuAc). TLC of isolated X2 revealed that it migrated between GD1a and GD2. On the other hand, X3 had a migratory rate on TLC between and GD1b and GT1b. Since both X2 and X3 were recognized by IgG anti-GalNAc-GD1a antibody, the results suggest that X2 is a GalNAc-GD1a species containing a mixture containing a NeuAc-and an N-glycolylneuraminic acid (NeuGc) species, and X3 is a GalNAc-GD1a species with two NeuGc. This evidence indicating the specific localization of GalNAc-GD1a and its isomers in spinal motor neurons should be useful in elucidating the pathogenic role of IgG anti-GalNAc-GD1a antibody in pure motor-type GBS.
Yoshino
Hiide
H
Department of Neurology, Kohnodai Hospital, National Center of Neurology and Psychiatry, 1-7-1 Kohnodai, Ichikawa, Chiba 2720-8516, Japan.
Ariga
Toshio
T
Suzuki
Akemi
A
Yu
Robert K
RK
Miyatake
Tadashi
T
D000818:Animals; D001323:Autoantibodies / Q000097:blood / Q000276:immunology; D002417:Cattle; D002845:Chromatography / Q000379:methods; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003637:DEAE-Dextran / Q000737:chemistry; D005677:G(M1) Ganglioside / Q000276:immunology / Q000378:metabolism; D005678:G(M2) Ganglioside / Q000276:immunology / Q000378:metabolism; D005679:G(M3) Ganglioside / Q000276:immunology / Q000378:metabolism; D005732:Gangliosides / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D009046:Motor Neurons / Q000166:cytology / Q000276:immunology* / Q000502:physiology; D010525:Peripheral Nerves / Q000276:immunology; D013116:Spinal Cord / Q000166:cytology / Q000276:immunology / Q000601:surgery
D001323:Autoantibodies; D005679:G(M3) Ganglioside; D005732:Gangliosides; D007074:Immunoglobulin G; C021298:ganglioside, GD1a; C033851:ganglioside, GD1b; D005678:G(M2) Ganglioside; D005677:G(M1) Ganglioside; C026226:ganglioside, GD3; C019403:ganglioside, GD2; C055253:IV(4)-galactosyl-N-acetylganglioside GD1a; D003637:DEAE-Dextran