title: Characterization of poly(4-vinylpyridine 1-oxide) by free-solution capillary electrophoresis and micellar electrokinetic chromatography.
abstract: The migration characteristics of poly(4-vinylpyridine 1-oxide) (PVP-NO) in phosphate buffers of acidic pH (20 mM H3PO4 or NaH2PO4) have been studied using both free-solution capillary electrophoresis (FSCE) and MEKC. To inhibit adsorption, 250 mM o-phosphoethanolamine (2-aminoethyl dihydrogen phosphate) was used. In FSCE, PVP-NO showed a narrow peak and a broader band, both having anionic behavior. These peak and band were attributed to the free and aggregated or micellized PVP-NO forms, respectively. According to surface tension measurements, the CMC of SDS in the BGE was 1.8 and 0.48 mM in the absence and in the presence of 1000 microg/mL PVP-NO, respectively, and the association of the polymer with SDS was completed at 9.7 mM SDS. Using MEKC, a narrow peak and a broader band also appeared at SDS concentrations of ca. 1 mM, and their intensity increased with the SDS concentration. These peak and band were attributed to the formation of mixed micelles constituted by both free PVP-NO/SDS and aggregated PVP-NO/SDS, respectively. The determination of PVP-NO by FSCE in commercial additives for laundry was demonstrated.
authors.affiliation: Departament de Química Analítica, Facultat de Química, Universitat de València, Burjassot, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Beneito-Cambra
authors.forename: José M
authors.initials: JM
authors.lastname: Herrero-Martínez
authors.forename: Guillermo
authors.initials: G
authors.lastname: Ramis-Ramos
mesh_terms: D000838:Anions; D002021:Buffers; D002845:Chromatography / Q000379:methods*; D003902:Detergents / Q000494:pharmacology; D019075:Electrophoresis, Capillary / Q000379:methods*; D007846:Laundering; D008823:Micelles; D010755:Organophosphates; D011144:Polyvinylpyridine N-Oxide / Q000031:analogs & derivatives* / Q000032:analysis; D012996:Solutions; D013500:Surface Tension; D014867:Water
chemical_list: C526585:2-aminoethyl phosphate; D000838:Anions; D002021:Buffers; D003902:Detergents; D008823:Micelles; D010755:Organophosphates; D012996:Solutions; C533089:poly(4-vinylpyridine-1-oxide); D014867:Water; D011144:Polyvinylpyridine N-Oxide