title: Noninvasive and quantitative monitoring of adult neuronal stem cell migration in mouse brain using bioluminescence imaging.
abstract: It is now generally accepted that continuous neurogenesis occurs in the adult mammalian brain, including that of humans. Modulation of adult neurogenesis can provide therapeutic benefits for various brain disorders, including stroke and Parkinson's disease. The subventricular zone-olfactory bulb pathway is one of the preferred model systems by which to study neural stem cell proliferation, migration, and differentiation in adult rodent brain. Research on adult neurogenesis would greatly benefit from reliable methods for long-term noninvasive in vivo monitoring. We have used lentiviral vectors encoding firefly luciferase to stably mark endogenous neural stem cells in the mouse subventricular zone. We show that bioluminescence imaging (BLI) allows quantitative follow-up of the migration of adult neural stem cells into the olfactory bulb in time. Moreover, we propose a model to fit the kinetic data that allows estimation of migration and survival times of the neural stem cells using in vivo BLI. Long-term expression of brain-derived neurotrophic factor in the subventricular zone attenuated neurogenesis, as detected by histology and BLI. In vivo monitoring of the impact of drugs or genes on adult neurogenesis is now within reach.
authors.affiliation: Neurobiology and Gene Therapy, Katholieke Universiteit Leuven, Leuven, Flanders, Belgium.
authors.forename: Veerle
authors.initials: V
authors.lastname: Reumers
authors.forename: Christophe M
authors.initials: CM
authors.lastname: Deroose
authors.forename: Olga
authors.initials: O
authors.lastname: Krylyshkina
authors.forename: Johan
authors.initials: J
authors.lastname: Nuyts
authors.forename: Martine
authors.initials: M
authors.lastname: Geraerts
authors.forename: Luc
authors.initials: L
authors.lastname: Mortelmans
authors.forename: Rik
authors.initials: R
authors.lastname: Gijsbers
authors.forename: Chris
authors.initials: C
authors.lastname: Van den Haute
authors.forename: Zeger
authors.initials: Z
authors.lastname: Debyser
authors.forename: Veerle
authors.initials: V
authors.lastname: Baekelandt
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology* / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000166:cytology* / Q000378:metabolism; D002465:Cell Movement; D003952:Diagnostic Imaging; D005260:Female; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008163:Luminescent Measurements; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009833:Olfactory Pathways / Q000166:cytology / Q000378:metabolism
chemical_list: D008156:Luciferases