High-performance liquid chromatographic determination of propylthiouracil in human urine by post-column iodine-azide reaction.
A sensitive, selective and simple post-column detection method for the determination of propylthiouracil (PTU) based on its sensitizing induction on iodine-azide reaction and the combination technique of high-performance liquid chromatography has been presented. The analysis was conducted in the optimum conditions for iodine-azide detection system and HPLC separation. The linear range, the lower limit of detection and quantification for PTU in urine were established at the levels of 0.4-1.0 nmol/ml urine, 0.3 nmol/ml urine and 0.4 nmol/ml urine, respectively.
Zakrzewski
Robert
R
Department of Instrumental Analysis, University of Łódź, Pomorska 163, 90-236 Łódź, Poland. robzak@chemul.uni.lodz.pl
D013956:Antithyroid Agents / Q000652:urine*; D001386:Azides / Q000737:chemistry*; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005260:Female; D006801:Humans; D007455:Iodine / Q000737:chemistry*; D008875:Middle Aged; D011441:Propylthiouracil / Q000652:urine*; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D013956:Antithyroid Agents; D001386:Azides; D011441:Propylthiouracil; D007455:Iodine