title: He use of the antibodies in the diagnosis of leukemia and lymphoma by flow cytometry.
abstract: Flow cytometry is an automated analysis of cells passing in the fluid suspension through a laser light beam, which react with monoclonal antibodies specific for a variety of cell surface antigens. A specimen of peripheral blood, bone marrow, or other cell suspension is incubated with fluorescent-labeled antibodies, which bind to target antigens on cell surfaces or--following cell permeabilization--to cytoplasmic and nuclear antigens. The analysis of surface antigens is performed on cells selected (gated) based on light-scatter properties. The expression of specific marker or confirmation of markers defines a specific cell population or the original of these cells. This in turn helps in diagnosis and classification of various hematological diseases and leads to choosing a specific therapy. Here, we describe a methodology for using flow cytometry with six colors for the analysis of various tissues for hematological diseases.
authors.affiliation: Department of Hematopathology, Quest Diagnostics Nichols Institute, San Juan Capistrano, CA, USA.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Sheikholeslami
authors.forename: Iman
authors.initials: I
authors.lastname: Jilani
authors.forename: Maher
authors.initials: M
authors.lastname: Albitar
mesh_terms: D000906:Antibodies; D000912:Antibodies, Neoplasm / Q000276:immunology; D002469:Cell Separation / Q000379:methods; D002470:Cell Survival; D005434:Flow Cytometry / Q000379:methods*; D006801:Humans; D007938:Leukemia / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D008223:Lymphoma / Q000175:diagnosis* / Q000276:immunology / Q000473:pathology; D013048:Specimen Handling / Q000592:standards
chemical_list: D000906:Antibodies; D000912:Antibodies, Neoplasm