title: Enzymes involved in the anoxic utilization of phenyl methyl ethers by Desulfitobacterium hafniense DCB2 and Desulfitobacterium hafniense PCE-S.
abstract: Phenyl methyl ethers are utilized by Desulfitobacterium hafniense DCB2 and Desulfitobacterium hafniense PCE-S; the methyl group derived from the O-demethylation of these substrates can be used as electron donor for anaerobic fumarate respiration or dehalorespiration. The activity of all enzymes involved in the oxidation of the methyl group to carbon dioxide via the acetyl-CoA pathway was detected in cell extracts of both strains. In addition, a carbon monoxide dehydrogenase activity could be detected. Activity staining of this enzyme indicated that the enzyme is a bifunctional CO dehydrogenase/acetyl-CoA synthase.
authors.affiliation: Institute of Microbiology, Chair of Applied and Ecological Microbiology Friedrich Schiller University Jena, Philosophenweg 12, 07743 Jena, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Kreher
authors.forename: Anke
authors.initials: A
authors.lastname: Schilhabel
authors.forename: Gabriele
authors.initials: G
authors.lastname: Diekert
mesh_terms: D000693:Anaerobiosis; D000840:Anisoles / Q000378:metabolism*; D001426:Bacterial Proteins / Q000378:metabolism*; D002245:Carbon Dioxide / Q000378:metabolism; D045858:Desulfitobacterium / Q000201:enzymology* / Q000378:metabolism; D004798:Enzymes / Q000378:metabolism*; D053858:Metabolic Networks and Pathways; D008954:Models, Biological
chemical_list: D000840:Anisoles; D001426:Bacterial Proteins; D004798:Enzymes; D002245:Carbon Dioxide; C060998:anisole