title: Crystallization and preliminary crystallographic analysis of merohedrally twinned crystals of MJ0729, a CBS-domain protein from Methanococcus jannaschii.
abstract: CBS domains are small protein motifs, usually associated in tandem, that are implicated in binding to adenosyl groups. Several genetic diseases in humans have been associated with mutations in CBS sequences, which has made them very promising targets for rational drug design. Trigonal crystals of the CBS-domain protein MJ0729 from Methanococcus jannaschii were grown by the vapour-diffusion method at acidic pH. Preliminary analysis of nine X-ray diffraction data sets using Yeates statistics and Britton plots showed that slight variation in the pH as well as in the buffer used in the crystallization experiments led to crystals with different degrees of merohedral twinning that may vary from perfect hemihedral twinning to perfect tetartohedral twinning.
authors.affiliation: Unidad de Cristalografía Macromolecular, CIC bioGUNE, Parque Tecnológico de Vizcaya, Ed. 800, 48160 Derio, Vizcaya, Spain.
authors.forename: Pablo
authors.initials: P
authors.lastname: Fernández-Millán
authors.forename: Danel
authors.initials: D
authors.lastname: Kortazar
authors.forename: María
authors.initials: M
authors.lastname: Lucas
authors.forename: María Luz
authors.initials: ML
authors.lastname: Martínez-Chantar
authors.forename: Egoitz
authors.initials: E
authors.lastname: Astigarraga
authors.forename: José Andrés
authors.initials: JA
authors.lastname: Fernández
authors.forename: Olatz
authors.initials: O
authors.lastname: Sabas
authors.forename: Armando
authors.initials: A
authors.lastname: Albert
authors.forename: Jose M
authors.initials: JM
authors.lastname: Mato
authors.forename: Luis Alfonso
authors.initials: LA
authors.lastname: Martínez-Cruz
mesh_terms: D020816:Amino Acid Motifs; D019843:Archaeal Proteins / Q000737:chemistry* / Q000302:isolation & purification; D003460:Crystallization; D018360:Crystallography, X-Ray; D003541:Cystathionine beta-Synthase / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D017017:Methanococcus / Q000737:chemistry*; D017434:Protein Structure, Tertiary; D053838:Scattering, Small Angle; D013237:Stereoisomerism
chemical_list: D019843:Archaeal Proteins; D003541:Cystathionine beta-Synthase