In vivo manipulation of fluorescently labeled organelles in living cells by multiphoton excitation.
Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein.
Watanabe
Wataru
W
National Institute of Advanced Industrial Science and Technology, Photonics Research Institute, 1-8-31, Midorigaoka, Ikeda, Osaka 563-8577, Japan. wataru.watanabe@aist.go.jp
Matsunaga
Sachihiro
S
Higashi
Tsunehito
T
Fukui
Kiichi
K
Itoh
Kazuyoshi
K
D036681:Fluorescence Recovery After Photobleaching / Q000379:methods*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism*; D006367:HeLa Cells; D006657:Histones / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008846:Micromanipulation / Q000379:methods*; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D015388:Organelles / Q000378:metabolism* / Q000648:ultrastructure*; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D013194:Staining and Labeling; D014026:Nicotiana / Q000166:cytology / Q000235:genetics / Q000378:metabolism
D006657:Histones; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins