Beneficial effects of fructose 1,6-biphosphate on hypothermia-induced reactive oxygen species injury in rats.
The release of reactive oxygen species has been described in hypothermic cells and tissues. Fructose 1,6-biphosphate (F1,6-BP) protects tissue stored at cold temperatures. We study the effect of F1,6-BP in vivo administration on anaesthetized rats exposed to cold stress (4 degrees C chamber for 30 min) and rewarming, to see if it alters cold-induced oxidative injury. Body temperatures show that the animals reached moderate hypothermia (26.80+/-0.62 degrees C) after 30 min of cold exposition. A decrease in mean arterial pressure was found. One group of animals was then rewarmed. Both hypothermia and rewarming increased the production of thiobarbituric acid-reactive substances, an index of lipid peroxidation, and reduced the antioxidant levels of plasmatic sulfhydryl groups, as well as decreasing the enzymatic activities of Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase and GSH peroxidase in erythrocytes. Administration of F1,6-BP increased sulfhydryl groups and limited lipid peroxidation in plasma. It furthermore enhanced Cu,Zn-SOD and GSH peroxidase antioxidant activity in erythrocytes and preserved mean arterial pressure. Therefore, F1,6-BP has therapeutic potential based on its ability to reduce free-radical injury resulting from acute cold exposure and rewarming in vivo.
Gámez
Antonio
A
Departament d'Enginyeria Química EUETIB, Universitat Politècnica de Catalunya, Barcelona, Spain.
Alva
Norma
N
Roig
Teresa
T
Bermúdez
Jordi
J
Carbonell
Teresa
T
D000818:Animals; D004912:Erythrocytes / Q000201:enzymology; D005635:Fructosediphosphates / Q000494:pharmacology* / Q000627:therapeutic use; D007035:Hypothermia / Q000188:drug therapy / Q000378:metabolism*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism*; D013438:Sulfhydryl Compounds / Q000032:analysis
D005635:Fructosediphosphates; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; C029063:fructose-1,6-diphosphate