Species-specific variation in efficacy of yeast genomic DNA isolation techniques assessed using amplified fragment length polymorphism.
Methods for isolating genomic DNA from yeasts are optimized for strains of Saccharomyces cerevisiae. The DNeasy tissue kit proved to be effective with 65 additional yeast species, providing 0.1 to 4.7 microg DNA/ml culture with sufficient purity to give reproducible amplified fragment length polymorphism (AFLP) profiles, but was unsuccessful with 13 other species. Two alternative yeast DNA purification kits, MasterPure and Y-DER, were effective with 6 of these and 2 additional species, leaving only 9 species that remained recalcitrant to yielding sufficient amounts of DNA with the required purity.
Fuller
Linda J
LJ
National Collection of Yeast Cultures, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
MacKenzie
Donald A
DA
Roberts
Ian N
IN
D004271:DNA, Fungal / Q000302:isolation & purification*; D005453:Fluorescence; D016681:Genome, Fungal / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length; D011933:Reagent Kits, Diagnostic; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D013045:Species Specificity
D004271:DNA, Fungal; D011933:Reagent Kits, Diagnostic