Displacement effects in large-scale chromatography?
Displacement effects in large-scale (total column volume v(t) = 150 L) and preparative ion-exchange chromatography purifying human erythrocyte superoxide dismutase are described in the present article. The biomolecules are eluted in a very small peak elution volume (<0.2 v(o)) behind the salt wave using a step gradient. The theoretical peak width and retention behavior are calculated according to the model of Yamamoto. The theoretical values are then compared with the experimental data. There was a difference observed between the elution type I (also called fronting) and the experimentally obtained elution. Some instructions are given on how to achieve these phenomenona because a beneficial effect in respect to resolution and recovery of a biomolecule is observed.
Jungbauer
A
A
Institute of Applied Microbiology, University of Bodenkultur, Nussdorferlaende 11 82, A-1190 Vienna, Austria.
Uhl
K
K
Schulz
P
P
Tauer
C
C
Gruber
G
G
Steindl
F
F
Buchacher
A
A
Schoenhofer
W
W
Unterluggauer
F
F