Detection and relative quantification of proteins by surface enhanced Raman using isotopic labels.
Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix format without interference from protein features.
Deb
Shirshendu K
SK
Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, West Lafayette, Indiana 47907, USA.
Davis
Brandon
B
Knudsen
Giselle M
GM
Gudihal
Ravindra
R
Ben-Amotz
Dor
D
Davisson
V Jo
VJ
D005456:Fluorescent Dyes / Q000737:chemistry*; D007553:Isotope Labeling; D011506:Proteins / Q000032:analysis*; D012235:Rhodamines / Q000737:chemistry*; D013059:Spectrum Analysis, Raman / Q000379:methods*
D005456:Fluorescent Dyes; D011506:Proteins; D012235:Rhodamines; C026188:rhodamine 6G