title: Simultaneous multifocal, multiphoton, photon counting microscopy.
abstract: We demonstrate a novel multifocal, multiphoton microscope that is capable of simultaneous dynamic imaging of multiple focal planes. We show for the first time that multimodal, multiphoton images excited with orthogonal polarizations can be acquired simultaneously in both the transmission and epi directions.
authors.affiliation: Department of Physics, Colorado School of Mines, 1523 Illinois Street, Golden, Colorado 80401, USA. rcarrile@mines.edu
authors.forename: Ramón
authors.initials: R
authors.lastname: Carriles
authors.forename: Kraig E
authors.initials: KE
authors.lastname: Sheetz
authors.forename: Erich E
authors.initials: EE
authors.lastname: Hoover
authors.forename: Jeff A
authors.initials: JA
authors.lastname: Squier
authors.forename: Virginijus
authors.initials: V
authors.lastname: Barzda
mesh_terms: D000465:Algorithms; D004867:Equipment Design; D007091:Image Processing, Computer-Assisted; D007834:Lasers; D008027:Light; D008853:Microscopy / Q000295:instrumentation* / Q000379:methods; D036641:Microscopy, Fluorescence, Multiphoton / Q000295:instrumentation* / Q000379:methods; D055095:Optics and Photonics; D017785:Photons; D012815:Signal Processing, Computer-Assisted; D013997:Time Factors