title: Prosthecobacter fluviatilis sp. nov., which lacks the bacterial tubulin btubA and btubB genes.
abstract: Leptothrix cholodnii is a sheathed bacterium often found in metal-rich and oligotrophic aquatic environments. A bacterial strain that is able to degrade the NaOH-treated sheath of L. cholodnii was isolated. The isolate was a Gram-negative, aerobic and prosthecate bacterium. The optimum growth temperature and pH were 30 degrees C and pH 7.0, respectively. The DNA G+C content was 62.9 mol%. The major respiratory quinone was MK-6. A phylogenetic analysis based on the 16S rRNA gene indicated that the isolate is a member of the genus Prosthecobacter. The nearest relative was the type strain of Prosthecobacter vanneervenii, with a similarity of 97.1 %. However, the isolate does not possess the bacterial tubulin genes, btubA and btubB, unique to known species of the genus Prosthecobacter. It is proposed that the isolate represents a novel species, Prosthecobacter fluviatilis sp. nov. The type strain is HAQ-1(T) (=JCM 14805(T) =KACC 12649(T) =KCTC 22182(T)).
authors.affiliation: Division of Materials Science and Chemical Engineering, Faculty of Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya, Yokohama 240-8501, Japan. mtake@ynu.ac.jp
authors.forename: Minoru
authors.initials: M
authors.lastname: Takeda
authors.forename: Akiko
authors.initials: A
authors.lastname: Yoneya
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Miyazaki
authors.forename: Keiko
authors.initials: K
authors.lastname: Kondo
authors.forename: Hiroko
authors.initials: H
authors.lastname: Makita
authors.forename: Masashi
authors.initials: M
authors.lastname: Kondoh
authors.forename: Ichiro
authors.initials: I
authors.lastname: Suzuki
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Koizumi
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D050260:Carbohydrate Metabolism; D005798:Genes, Bacterial / Q000235:genetics*; D046529:Microscopy, Electron, Transmission; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity; D014404:Tubulin / Q000235:genetics*
chemical_list: D012336:RNA, Ribosomal, 16S; D014404:Tubulin