Ultrafast reversible photo-cross-linking reaction: toward in situ DNA manipulation.
We describe a novel ultrafast reversible DNA interstrand photo-cross-linking reaction via 3-cyanovinylcarbazole nucleoside ( (CNV)K). Oligodeoxynucleotide (ODN) containing (CNV)K can be photo-cross-linked by irradiation at 366 nm for 1 s, and the photo-cross-linked ODN can be split by irradiation at 312 nm for 60 s.
Yoshimura
Yoshinaga
Y
School of Materials Science, Japan Advanced Institute of Science and Technology, 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.
Fujimoto
Kenzo
K
D002227:Carbazoles / Q000737:chemistry / Q000528:radiation effects; D002851:Chromatography, High Pressure Liquid; D003432:Cross-Linking Reagents / Q000737:chemistry* / Q000528:radiation effects; D004247:DNA / Q000737:chemistry* / Q000528:radiation effects*; D009570:Nitriles / Q000737:chemistry / Q000528:radiation effects; D009705:Nucleosides / Q000737:chemistry / Q000528:radiation effects; D009841:Oligonucleotides / Q000737:chemistry / Q000528:radiation effects; D010777:Photochemistry; D014753:Vinyl Compounds / Q000737:chemistry / Q000528:radiation effects
D002227:Carbazoles; D003432:Cross-Linking Reagents; D009570:Nitriles; D009705:Nucleosides; D009841:Oligonucleotides; D014753:Vinyl Compounds; D004247:DNA