Reference gene selection for real-time RT-PCR in regenerating mouse livers.
The liver has an intrinsic ability to undergo active proliferation and recover functional liver mass in response to an injury response. This regenerative process involves a complex yet well orchestrated change in the gene expression profile. To produce accurate and reliable gene expression of target genes during various stages of liver regeneration, the determination of internal control housekeeping genes (HKGs) those are uniformly expressed is required. In the present study, the gene expression of 8 commonly used HKGs, including GAPDH, ACTB, HPRT1, GUSB, PPIA, TBP, TFRC, and RPL4, were studied using mouse livers that were quiescent and actively regenerating induced by partial hepatectomy. The amplification of the HKGs was statistically analyzed by two different mathematical algorithms, geNorm and NormFinder. Using this method, PPIA and TBP gene expression found to be relatively stable regardless of the stages of liver regeneration and would be ideal for normalization to target gene expression.
Tatsumi
Kohei
K
Department of Pediatrics, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
Ohashi
Kazuo
K
Taminishi
Sanae
S
Okano
Teruo
T
Yoshioka
Akira
A
Shima
Midori
M
D000818:Animals; D005260:Female; D020869:Gene Expression Profiling / Q000592:standards*; D008115:Liver Regeneration / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards*