Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside.
Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5'-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.
Wei
Xiao-Kun
XK
College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China. weixiaokun99@163.com
Ding
Qing-Bao
QB
Zhang
Lu
L
Guo
Yong-Li
YL
Ou
Lin
L
Wang
Chang-Lu
CL
D003562:Cytidine / Q000494:pharmacology; D003568:Cytidine Monophosphate / Q000494:pharmacology; D021902:Enterobacter aerogenes / Q000201:enzymology*; D004790:Enzyme Induction; D010430:Pentosyltransferases / Q000096:biosynthesis*; D014740:Vidarabine / Q000096:biosynthesis*
D003562:Cytidine; D010430:Pentosyltransferases; C067995:nucleoside phosphorylase; D003568:Cytidine Monophosphate; D014740:Vidarabine