[High glucose suppresses ABCG1 expression by increasing oxidative stress and inducing nuclear factor-kappaB activation in vascular smooth muscle cells].
OBJECTIVE
To investigate the role of high glucose in the expression of ATP-binding cassette (ABC) transporters A1 (ABCA1) and G1 (ABCG1) in human vascular smooth muscle cells (VSMCs) and its possible mechanisms.


METHODS
VSMCs were incubated in the presence of glucose at the concentrations ranging from 5 to 30 mmol/L for 1 to 7 days, and real-time PCR and Western blotting were used to measure the mRNA and protein expressions of ABCA1 and ABCG1. The effects of cells pretreatment with antioxidant NAC (10 mmol/L) and nuclear factor-kappaB (NF-kappaB) inhibitors BAY 11-7085 (10 micromol/L) and TPCK (10 micromol/L) were also tested on ABCA1 and ABCG1 expressions.


RESULTS
High glucose suppressed, in a time- and dose-dependent manner, ABCG1 expression in incubated human VSMCs, and this effect was abolished by pretreatment with the antioxidant and nuclear factor-kappaB (NF-kappaB) inhibitors, but ABCA1 expression was not significantly decreased in the presence of high glucose.


CONCLUSIONS
High glucose suppresses ABCG1 expression in human VSMCs possibly due to increased oxidative stress and NF-kappaB activation induced by high glucose.
Xue
Jia-Hong
JH
Department of Cardiovascular Medicine, First Affiliated Hospital, Key Laboratory of Environment and Gene Related Disease of Ministry Education, Xi'an Jiaotong University College of Medicine, Xi'an 710061, China. xuejiahong@gmail.com
Yuan
Zu-Yi
ZY
Wu
Yue
Y
Zhao
Yan
Y
D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters / Q000096:biosynthesis* / Q000235:genetics; D001011:Aorta / Q000166:cytology; D015153:Blotting, Western; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D015536:Down-Regulation; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction
C577836:ABCA1 protein, human; C419353:ABCG1 protein, human; D064286:ATP Binding Cassette Transporter 1; D000070998:ATP Binding Cassette Transporter, Subfamily G, Member 1; D018528:ATP-Binding Cassette Transporters; D016328:NF-kappa B; D012333:RNA, Messenger; D005947:Glucose