[Determination of three bufogenins in toad venom using reversed-phase high performance liquid chromatography].
A reversed-phase high performance liquid chromatographic method for the quantitative determination of three bufogenins in toad venom was established. An Alltech Alltima C18 column (250 mm x 4.6 mm, 5 microm) was used. The mobile phase was acetonitrile-0.1 mol/L acetate buffer (pH 3.2) (50:50, v/v), and the flow rate was 0.8 mL/min. The detection wavelength was set at 299 nm. The method has good linearity in the ranges of 0. 25 -0.875 microg for cinobufagin (r = 0.999 0), 0.25 -0.875 microg for resibufogenin (r = 0.999 1), and 0.15 - 0.525 microg for bufalin (r = 0. 999 0). The average recoveries were 100.3%, 100.0% and 98.0% for cinobufagin, resibufogenin, and bufalin, respectively. The results indicate that the method is simple, accurate, reproducible, and can be used for the quality control of bufogenins in toad venom.
Liu
Jihua
J
Department of Traditional Chinese Prescription, School of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China. jihualiu88@163.com
Wang
Jingrong
J
Yu
Boyang
B
D000664:Amphibian Venoms / Q000737:chemistry*; D002018:Bufanolides / Q000032:analysis* / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D016014:Linear Models; D011786:Quality Control; D015203:Reproducibility of Results; D013997:Time Factors
D000664:Amphibian Venoms; D002018:Bufanolides; C005734:bufogenin