title: Sequential and quantitative analysis of a murine model of elastase-induced emphysema.
abstract: Emphysema, one of chronic obstructive pulmonary disease (COPD), is characterized as destruction of airway wall and small airway inflammation. To assess the kinetics of disease progression in murine model of elastase-induced emphysema, we used micro-computed tomography (CT) compared with morphological changes. Two week after elastase administration, a significant increase in the volume of low-density areas, recognized as -800--600 Hounsfield units by micro-CT, was observed. Coefficient of correlation between mean linear intercept (Lm) and low-density area examined by CT, was 0.79 (p<0.01). Micro-CT can quantitatively and sequentially detect murine emphysematous changes, offering a practical method to sequentially analyze the therapeutic effects of treatments in a murine model of emphysema.
authors.affiliation: Department of Pharmatherapy, Research Institute of Pharmaceutical Sciences, Musashino University, Tokyo, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Kawakami
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Matsuo
authors.forename: Kenta
authors.initials: K
authors.lastname: Yoshiura
authors.forename: Takahide
authors.initials: T
authors.lastname: Nagase
authors.forename: Naomi
authors.initials: N
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D004646:Emphysema / Q000139:chemically induced* / Q000473:pathology*; D007091:Image Processing, Computer-Assisted; D007700:Kinetics; D051379:Mice; D008810:Mice, Inbred C57BL; D010196:Pancreatic Elastase; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D014057:Tomography, X-Ray Computed
chemical_list: D012333:RNA, Messenger; D010196:Pancreatic Elastase