Transcriptome analysis of auxin-regulated genes of Arabidopsis thaliana.
Plant hormone auxin elicits diverse responses in plant growth and development. Accumulated data indicate that the ubiquitin-mediated proteolytic pathway plays a crucial role in transducing auxin signaling. To gain more understanding of the molecular mechanisms underlying auxin action, we performed a comparative transcriptome analysis of auxin responsive genes between Arabidopsis Columbia ecotype and the auxin insensitive mutant eta2 by cDNA-AFLP. Using 256 primer combinations, about 5900 transcript-derived fragments (TDFs) were amplified. Sixty-six differentially expressed TDFs were confirmed by DNA dot blot analysis. Sequence analysis indicated that, a large number of genes involved in transcription regulation or RNA metabolism were identified as auxin-regulated genes. Northern blot analyses confirmed transcription levels of 16 auxin-regulated genes. These genes include various forms of transcription regulators, defense related, RING-type ubiquitin ligases, and glycosyl hydrolase. This study demonstrates that auxin exerts its effect in complex transcriptional networks.
Huang
Yung-Chieh
YC
Department of BioAgricultural Sciences, National Chiayi University, Chiayi, Taiwan.
Chang
Yueh-Long
YL
Hsu
Jen-Jen
JJ
Chuang
Huey-wen
HW
D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D018076:DNA, Complementary / Q000378:metabolism; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007210:Indoleacetic Acids / Q000494:pharmacology*; D008969:Molecular Sequence Data; D010937:Plant Growth Regulators / Q000494:pharmacology*; D016415:Sequence Alignment; D014158:Transcription, Genetic / Q000187:drug effects
D029681:Arabidopsis Proteins; D018076:DNA, Complementary; D007210:Indoleacetic Acids; D010937:Plant Growth Regulators