title: Comprehensive analysis of T cell epitope discovery strategies using 17DD yellow fever virus structural proteins and BALB/c (H2d) mice model.
abstract: Immunomics research uses in silico epitope prediction, as well as in vivo and in vitro approaches. We inoculated BALB/c (H2d) mice with 17DD yellow fever vaccine to investigate the correlations between approaches used for epitope discovery: ELISPOT assays, binding assays, and prediction software. Our results showed a good agreement between ELISPOT and binding assays, which seemed to correlate with the protein immunogenicity. PREDBALB/c prediction software partially agreed with the ELISPOT and binding assay results, but presented low specificity. The use of prediction software to exclude peptides containing no epitopes, followed by high throughput screening of the remaining peptides by ELISPOT, and the use of MHC-biding assays to characterize the MHC restrictions demonstrated to be an efficient strategy. The results allowed the characterization of 2 MHC class I and 17 class II epitopes in the envelope protein of the YF virus in BALB/c (H2d) mice.
authors.affiliation: Johns Hopkins University, School of Medicine, Pharmacology Department, Baltimore, USA.
authors.forename: Milton
authors.initials: M
authors.lastname: Maciel
authors.forename: Srinivasan N
authors.initials: SN
authors.lastname: Kellathur
authors.forename: Pryia
authors.initials: P
authors.lastname: Chikhlikar
authors.forename: Rafael
authors.initials: R
authors.lastname: Dhalia
authors.forename: John
authors.initials: J
authors.lastname: Sidney
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sette
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: August
authors.forename: Ernesto T A
authors.initials: ET
authors.lastname: Marques
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018604:Epitope Mapping; D018984:Epitopes, T-Lymphocyte / Q000276:immunology*; D005260:Female; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D000949:Histocompatibility Antigens Class II / Q000378:metabolism; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D012984:Software; D014759:Viral Envelope Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism; D015678:Viral Structural Proteins / Q000737:chemistry / Q000276:immunology / Q000378:metabolism*; D015004:Yellow Fever / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D022341:Yellow Fever Vaccine / Q000008:administration & dosage / Q000276:immunology; D015005:Yellow fever virus / Q000276:immunology*
chemical_list: D018984:Epitopes, T-Lymphocyte; D015395:Histocompatibility Antigens Class I; D000949:Histocompatibility Antigens Class II; D014759:Viral Envelope Proteins; D015678:Viral Structural Proteins; D022341:Yellow Fever Vaccine