New cembranoid diterpenes from the Vietnamese soft coral Sarcophyton mililatensis stimulate osteoblastic differentiation in MC3T3-E1 cells.
Chemical investigation of the Vietnamese soft coral Sarcophyton mililatensis resulted in the isolation of four new cembranoid diterpenes, namely (-)-7beta-hydroxy-8alpha-methoxydeepoxysarcophytoxide (1), (+)-7beta,8beta-dihydroxydeepoxysarcophytoxide (2), (-)-17-hydroxysarcophytonin A (3) and sarcophytol V (4), along with two known compounds, (+)-sarcophine (5) and sarcophytoxide (6). The NMR spectral data of the new compounds were completely assigned by using a combination of 2D NMR experiments including (1)H-(1)H COSY, HSQC, HMBC, and ROESY. To investigate the bioactivities of compounds, which act on bone metabolism, we studied the effects of compounds on the function of osteoblastic MC3T3-E1 cells. Compound 1 caused a significant elevation of alkaline phosphatase activity, collagen content, and nodules mineralization compared to those of the control (p<0.05). These results suggest that newly isolated compound 1 has a direct stimulatory effect on bone formation in vitro and may contribute to the prevention for osteoporosis.
Cuong
Nguyen Xuan
NX
Institute of Natural Products Chemistry, Vietnamese Academy of Science and Technology, Hanoi, Vietnam.
Tuan
Tran Anh
TA
Kiem
Phan Van
PV
Minh
Chau Van
CV
Choi
Eun Mi
EM
Kim
Young Ho
YH
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D037421:Anthozoa / Q000737:chemistry*; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D003094:Collagen / Q000096:biosynthesis; D004224:Diterpenes / Q000737:chemistry / Q000494:pharmacology*; D009682:Magnetic Resonance Spectroscopy; D051379:Mice; D008810:Mice, Inbred C57BL; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects*
C530551:(-)-7beta-hydroxy-8alpha-methoxydeepoxysarcophytoxide; D004224:Diterpenes; D003094:Collagen; D000469:Alkaline Phosphatase