title: Experimental study of [99mTc(PNP5) (DBODC)]+ as a new myocardial perfusion imaging agent.
abstract: OBJECTIVE
  This study was aimed to compare the properties of [(99m)Tc(PNP5)(DBODC)]+ with (99m)Tc-MIBI in normal swine and in canine models of myocardial ischemia.
  
  
  METHODS
  Seven anesthetized swine were injected intravenously with [(99m)Tc(PNP5)(DBODC)]+. Serial planar images were acquired meanwhile. Twelve adult dogs were studied. A critical stenosis of left anterior descending artery was produced. [(99m)Tc(PNP5)(DBODC)]+ or (99m)Tc-MIBI (randomly) was injected intravenously at the end of 3 min of adenosine infusion. The occluder was released after adenosine infusion. Myocardial SPECT imaging was obtained at 30, 60, 90 and 180 min, respectively, after tracer injection. Rest myocardial SPECT imaging was acquired on the next day.
  
  
  RESULTS
  Blood disappearance of [(99m)Tc(PNP5)(DBODC)]+ was biexponential with an initial half-time of 2.97 +/- 0.48 min and a late half-time of 52.49 +/- 19.49 min. Myocardial [(99m)Tc(PNP5)(DBODC)]+ uptake was similar to (99m)Tc-MIBI. Heart-to-liver ratio of [(99m)Tc(PNP5)(DBODC)]+ was significantly higher than that of (99m)Tc-MIBI (0.99 +/- 0.03 vs. 0.46 +/- 0.10, p < 0.05) 30 min after injection. In canine models of myocardial ischemia, image quality of [(99m)Tc(PNP5)(DBODC)]+ was better than (99m)Tc-MIBI. Segments with perfusion defects by [(99m)Tc(PNP5)(DBODC)]+ was 3.60 +/- 1.52 and 4.25 +/- 0.96 by (99m)Tc-MIBI (p = n.s.).
  
  
  CONCLUSIONS
  [(99m)Tc(PNP5)(DBODC)]+ seems to be a promising new myocardial perfusion imaging agent.
authors.affiliation: Department of Nuclear Medicine, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 167 Bei Li Shi Lu, Beijing, China.
authors.forename: Wan-Chun
authors.initials: WC
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Fang
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Xue-Bin
authors.initials: XB
authors.lastname: Wang
authors.forename: Zuo-Xiang
authors.initials: ZX
authors.lastname: He
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D004285:Dogs; D006321:Heart / Q000000981:diagnostic imaging*; D017202:Myocardial Ischemia / Q000000981:diagnostic imaging*; D009943:Organophosphorus Compounds / Q000493:pharmacokinetics; D015609:Organotechnetium Compounds / Q000493:pharmacokinetics; D013552:Swine; D013556:Swine, Miniature; D017256:Technetium Tc 99m Sestamibi / Q000493:pharmacokinetics; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*
chemical_list: C496311:99mTc-N-DBODC5; D009943:Organophosphorus Compounds; D015609:Organotechnetium Compounds; D017256:Technetium Tc 99m Sestamibi