[Effects of subchronic exposure of laboratory mice to benzylbutyl phthalate (BBP) on the quantity and quality of male germ cells].
The aim of the study was to investigate the effect of 8-weeks exposure of male mice to benzyl butyl phthalate (BBP) on the sperm count and quality of gametes. Pzh:Sfis male mice exposed per os to 450 mg/kg bw (1/16 LD50) and to 1800 mg/kg bw (1/4 LD50) of BBP in olive oil were used in the study. Control mice were treated with olive oil only. Groups of animals were killed 4 and 8 weeks after the start of exposure and 4 weeks after he end of exposure. Sperm count, motility, morphology and DNA damage in gametes were estimated in the study. Sperm counts were diminished 4 and 8 weeks after the start of exposure to BBP. In the same time decrease in sperm motility and dose-dependent increase in the frequency of abnormal sperm heads and slight increase in DNA damage were noted. 4 weeks after the end of exposure, slight decrease in sperm counts in the group of 1/4 LD50 was observed, only. Correlation between sperm count and testes and epididymes weight were noted. Themost sensitive to BBP exposure occurred spermatozoa and spermatids.
Tyrkiel
Ewa J
EJ
Zakład Toksykologii Srodowiskowej Patistwowy Zaldad Higieny 00-791 Warszawa, ul. Chocimska 24.
Dobrzyńska
Małgorzata M
MM
Derezińska
Edyta
E
Ludwicki
Jan K
JK
D000818:Animals; D004785:Environmental Pollutants / Q000633:toxicity*; D004822:Epididymis / Q000187:drug effects; D008297:Male; D051379:Mice; D010795:Phthalic Acids / Q000633:toxicity*; D013076:Sperm Count; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects*; D013723:Teratogens / Q000633:toxicity*; D013737:Testis / Q000187:drug effects
D004785:Environmental Pollutants; D010795:Phthalic Acids; D013723:Teratogens; C027561:butylbenzyl phthalate