title: [Determination of three bufogenins in toad venom using reversed-phase high performance liquid chromatography].
abstract: A reversed-phase high performance liquid chromatographic method for the quantitative determination of three bufogenins in toad venom was established. An Alltech Alltima C18 column (250 mm x 4.6 mm, 5 microm) was used. The mobile phase was acetonitrile-0.1 mol/L acetate buffer (pH 3.2) (50:50, v/v), and the flow rate was 0.8 mL/min. The detection wavelength was set at 299 nm. The method has good linearity in the ranges of 0. 25 -0.875 microg for cinobufagin (r = 0.999 0), 0.25 -0.875 microg for resibufogenin (r = 0.999 1), and 0.15 - 0.525 microg for bufalin (r = 0. 999 0). The average recoveries were 100.3%, 100.0% and 98.0% for cinobufagin, resibufogenin, and bufalin, respectively. The results indicate that the method is simple, accurate, reproducible, and can be used for the quality control of bufogenins in toad venom.
authors.affiliation: Department of Traditional Chinese Prescription, School of Chinese Materia Medica, China Pharmaceutical University, Nanjing 210009, China. jihualiu88@163.com
authors.forename: Jihua
authors.initials: J
authors.lastname: Liu
authors.forename: Jingrong
authors.initials: J
authors.lastname: Wang
authors.forename: Boyang
authors.initials: B
authors.lastname: Yu
mesh_terms: D000664:Amphibian Venoms / Q000737:chemistry*; D002018:Bufanolides / Q000032:analysis* / Q000592:standards; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056148:Chromatography, Reverse-Phase / Q000379:methods*; D016014:Linear Models; D011786:Quality Control; D015203:Reproducibility of Results; D013997:Time Factors
chemical_list: D000664:Amphibian Venoms; D002018:Bufanolides; C005734:bufogenin