Taxonomic distribution of Streptomyces species capable of producing bioactive compounds among strains preserved at NITE/NBRC.
The taxonomic distribution of Streptomyces species capable of producing bioactive compounds was investigated. Nine hundred and six strains were tested for the following four biological activities: antimicrobial, anti-tyrosinase, antioxidant, and hemolytic. Approximately 30% of strains tested showed antimicrobial activities, except for anti-Escherichia coli activity, which was present in only a few strains, while the rates of positivity for the anti-tyrosinase, antioxidant, and hemolytic activities were much lower. The distribution of Streptomyces strains capable of producing bioactive compounds was analyzed by the taxonomy based on 16S rRNA gene sequences. Moreover, the strains of Streptomyces hygroscopicus tested were divided into two clades in the phylogenetic tree, and all of the strains belonging to one clade showed antibacterial and antifungal activities. For detection of polyenes, the UV-visible spectra of metabolic extracts in the strains showing antifungal activities were measured. It was suggested that Streptomyces strains produce universal active compounds under different growth conditions. Further information on the relationship between the microbial taxonomy and the bioactive compounds produced would be useful for the utilization of industrial microorganisms.
Anzai
Kozue
K
NITE Biological Research Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8, Kazusakamatari, Kisarazu-shi, Chiba, 292-0818, Japan.
Ohno
Michiyo
M
Nakashima
Takuji
T
Kuwahara
Natsumi
N
Suzuki
Rieko
R
Tamura
Tomohiko
T
Komaki
Hisayuki
H
Miyadoh
Shinji
S
Harayama
Shigeaki
S
Ando
Katsuhiko
K
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism*; D000935:Antifungal Agents / Q000378:metabolism*; D000975:Antioxidants / Q000378:metabolism*; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D004912:Erythrocytes / Q000382:microbiology; D053878:Hemolytic Agents / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D011817:Rabbits; D013302:Streptomyces / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*; D014481:United States; D037041:United States Government Agencies / Q000458:organization & administration
D000900:Anti-Bacterial Agents; D000935:Antifungal Agents; D000975:Antioxidants; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D053878:Hemolytic Agents; D010455:Peptides; D012336:RNA, Ribosomal, 16S