title: Bacterial growth on lactose: an experimental investigation.
abstract: A wild-type strain of Klebsiella oxytoca growing aerobically in batch culture has exhibited intermittent or oscillatory growth while growing on lactose at concentrations on the order of 1 g/L or less. In two-substrate experiments, preferred growth on glucose followed by growth on lactose also produced oscillatory growth behavior during the lactose growth phase at lactose concentrations of 1 g/L or less. Only oscillations in cell density have currently been observed. Alkalinization of the medium during growth on lactose indicated the presence of lactose active transport. The observed intermittent growth was reduced or removed during growth on lactose after preferred growth on galactose or in a medium containing 50 mM NaCl. Results suggested that the presence of an intracellular energy source or a sufficient DeltapH buffer may alleviate growth inhibition when transport and growth processes compete for essential energy resources during growth on lactose.
authors.affiliation: School of Chemical Engineering, Purdue University, West Lafayette, Indiana 47907, USA.
authors.forename: J V
authors.initials: JV
authors.lastname: Straight
authors.forename: D
authors.initials: D
authors.lastname: Ramkrishna
authors.forename: S J
authors.initials: SJ
authors.lastname: Parulekar
authors.forename: N B
authors.initials: NB
authors.lastname: Jansen