title: Continuous production of L-phenylalanine by transamination.
abstract: L-Phenylalanine was produced continuously from L-as-partate and phenylpyruvate by transaminase from a newly screened Pseudomonas putida strain. The process was carried out with an isolated enzyme in homogeneous phase in an enzyme membrane reactor and with immobilized whole cells in a stirred tank reactor, respectively. Due to the difference in transport resistance, the productivity of the free enzyme in homogeneous phase (72 mmol/L h) was about 3 times higher than the productivity achieved using immobilized cells. However, a better stability of the biocatalyst was observed with immobilized cells.
authors.affiliation: Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300 Braunschweig, FRG.
authors.forename: H
authors.initials: H
authors.lastname: Ziehr
authors.forename: M R
authors.initials: MR
authors.lastname: Kula
authors.forename: E
authors.initials: E
authors.lastname: Schmidt
authors.forename: C
authors.initials: C
authors.lastname: Wandrey
authors.forename: J
authors.initials: J
authors.lastname: Klein