[Determination of 1, 2, 3, 4, 6-penta-O-galloyl-D-glucose in forty four kinds of Chinese traditional medicines by HPLC].
OBJECTIVE
To determine 1, 2, 3, 4, 6-penta-O-galloyl-D-glucose (PGG) in forty four kinds of Chinese traditional medicines by HPLC.


METHODS
The PGG was extracted with a solvent consisting of ethanol and water (50:50) in ultrasonic bath at 50-60 degrees C for 1 hour. A Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was used for the separation and analysis of PGG with a mobile phase consisting of acetonitrile and 2. 5 % acetic acid solution (18:82). The wavelength of detection was at 280 nm, and flow rate was set at 1.0 mL min(-1).


RESULTS
The calibration curve for PGG is linear over the range of 1-150 microg mL(-1) (r =0.9994), and PGG was found in seventeen kinds of Chinese traditional medicines, such as gall tree peony bark, red peony root, white peony root, and so on.


CONCLUSIONS
The contents of PGG were determined in forty four kinds of Chinese traditional medicines by a rapid and precision HPLC method.
Wang
Wei-Cong
WC
School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China.
Wang
Chao
C
Song
Xue-Ying
XY
Zhao
Wen-Hua
WH
Wang
Qiao
Q
D002138:Calibration; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D047348:Hydrolyzable Tannins / Q000032:analysis*; D012680:Sensitivity and Specificity
D004365:Drugs, Chinese Herbal; D047348:Hydrolyzable Tannins; C435084:pentagalloylglucose