Regulation of the Helicobacter pylori cellular receptor decay-accelerating factor.
Chronic gastritis induced by Helicobacter pylori is the strongest known risk factor for peptic ulceration and distal gastric cancer, and adherence of H. pylori to gastric epithelial cells is critical for induction of inflammation. One H. pylori constituent that increases disease risk is the cag pathogenicity island, which encodes a secretion system that translocates bacterial effector molecules into host cells. Decay-accelerating factor (DAF) is a cellular receptor for H. pylori and a mediator of the inflammatory response to this pathogen. H. pylori induces DAF expression in human gastric epithelial cells; therefore, we sought to define the mechanism by which H. pylori up-regulates DAF and to extend these findings into a murine model of H. pylori-induced injury. Co-culture of MKN28 gastric epithelial cells with the wild-type H. pylori cag(+) strain J166 induced transcriptional expression of DAF, which was attenuated by disruption of a structural component of the cag secretion system (cagE). H. pylori-induced expression of DAF was dependent upon activation of the p38 mitogen-activated protein kinase pathway but not NF-kappaB. Hypergastrinemic INS-GAS mice infected with wild-type H. pylori demonstrated significantly increased DAF expression in gastric epithelium versus uninfected controls or mice infected with an H. pylori cagE(-) isogenic mutant strain. These results indicate that H. pylori cag(+) strains induce up-regulation of a cognate cellular receptor in vitro and in vivo in a cag-dependent manner, representing the first evidence of regulation of an H. pylori host receptor by the cag pathogenicity island.
O'Brien
Daniel P
DP
Division of Gastroenterology, Department of Medicine, Vanderbilt University School of Medicine, 2215 Garland Avenue, Nashville, TN 37232-2279, USA.
Romero-Gallo
Judith
J
Schneider
Barbara G
BG
Chaturvedi
Rupesh
R
Delgado
Alberto
A
Harris
Elizabeth J
EJ
Krishna
Uma
U
Ogden
Seth R
SR
Israel
Dawn A
DA
Wilson
Keith T
KT
Peek
Richard M
RM
D000818:Animals; D001426:Bacterial Proteins; D018958:CD55 Antigens / Q000096:biosynthesis*; D002460:Cell Line; D018920:Coculture Techniques; D005753:Gastric Mucosa / Q000378:metabolism* / Q000382:microbiology; D044404:Genomic Islands / Q000235:genetics; D016481:Helicobacter Infections / Q000235:genetics / Q000378:metabolism*; D016480:Helicobacter pylori / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D051379:Mice; D009154:Mutation; D014158:Transcription, Genetic / Q000235:genetics; D015854:Up-Regulation / Q000235:genetics
D001426:Bacterial Proteins; D018958:CD55 Antigens; C103937:PicB protein, Helicobacter pylori