Ion mobility-mass spectrometry analysis of large protein complexes.
Here we describe a detailed protocol for both data collection and interpretation with respect to ion mobility-mass spectrometry analysis of large protein assemblies. Ion mobility is a technique that can separate gaseous ions based on their size and shape. Specifically, within this protocol, we cover general approaches to data interpretation, methods of predicting whether specific model structures for a given protein assembly can be separated by ion mobility, and generalized strategies for data normalization and modeling. The protocol also covers basic instrument settings and best practices for both observation and detection of large noncovalent protein complexes by ion mobility-mass spectrometry.
Ruotolo
Brandon T
BT
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK. btr23@cam.ac.uk
Benesch
Justin L P
JL
Sandercock
Alan M
AM
Hyung
Suk-Joon
SJ
Robinson
Carol V
CV
D003627:Data Interpretation, Statistical; D017136:Ion Transport; D013058:Mass Spectrometry / Q000379:methods*; D008958:Models, Molecular; D015233:Models, Statistical; D046912:Multiprotein Complexes / Q000737:chemistry*
D046912:Multiprotein Complexes