Interleukin-12 gene modification exerts anti-tumor effects on murine mammary sarcoma cell line in vivo.
The aim of this project was to investigate the anti-tumor effect of an IL-12 gene modified mammary sarcoma murine cell line, EMT6/IL-12, in mouse model. In this study, we transfected the recombinant eukaryotic plasmid encoding IL-12 gene (pcDNA6-p70) into EMT6 and obtained the IL-12 expressing EMT6/IL-12 cell line. Then EMT6/IL-12 cells were s.c. inoculated into mice. The recombinant vector treatment group was set as control. We then evaluated the inhibition of tumor growth and the anti-tumor immunity function in vivo such as cytotoxicity, proliferation of splenocytes and serial IFN-gamma level. And the percentage of IFN-gamma producing CD4 or CD8 T cells among splenocytes was also analyzed in tumor bearing mice. Our results showed that the growth of tumors was obviously inhibited in EMT6/IL-12 group. Moreover, the capacities of anti-tumor immunity were all significantly higher in EMT6/IL-12 group compared to the controls. The results of the present investigation support the notion that EMT6/IL-12 could exert gene therapy in tumor model by improving the anti-tumor cellular immunity.
Li
Dan
D
Department of Microbiology, Medical College of Qingdao University, Qingdao, China.
Yu
Hong
H
Xu
Teng Fei
TF
Li
Jing Hua
JH
Sun
Yun Fang
YF
Zhang
Wen Qing
WQ
D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D003602:Cytotoxicity, Immunologic; D004195:Disease Models, Animal; D005260:Female; D015316:Genetic Therapy; D007371:Interferon-gamma / Q000096:biosynthesis / Q000276:immunology*; D018664:Interleukin-12 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D015674:Mammary Neoplasms, Animal / Q000276:immunology* / Q000378:metabolism / Q000628:therapy; D051379:Mice; D008815:Mice, Inbred Strains; D012509:Sarcoma / Q000276:immunology* / Q000378:metabolism / Q000628:therapy; D014162:Transfection
D018664:Interleukin-12; D007371:Interferon-gamma