title: High-Density culture of mouse-human hybridoma in serum-free defined medium.
abstract: Mouse-human hybridoma 4H11 cells producing anti-Pseudomonas sp. monoclonal antibody (IgA) grew in a serum-free medium supplemented with insulin, transferrin, ethanolamine, and selenite (ITES). The hybridoma could be applied to high-density culture in a serum-free medium supplemented with ITES, 0.5% BSA, egg yolk VLDL, and artificial blood FC-43 in a culture vessel equipped with hollow-fiber modules for medium exchange. Total cell density reached 1.1 x 10(7) cells/mL (viable cell density was 7.6 x 10(6) cells/mL), and the IgA productivity was around 20 mug/10(6) cells/day in the serum-free medium, which corresponded to the levels in serum-supplemented medium.
authors.affiliation: Biotechnology Research Laboratory Teijin Limited, Hino 191, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Takazawa
authors.forename: M
authors.initials: M
authors.lastname: Tokashiki
authors.forename: H
authors.initials: H
authors.lastname: Murakami
authors.forename: K
authors.initials: K
authors.lastname: Yamada
authors.forename: H
authors.initials: H
authors.lastname: Omura