Salicylate-induced morphological changes of isolated inner hair cells and outer hair cells from guinea-pig cochlea.
OBJECTIVE
The aim of this study is to investigate the salicylate-induced morphological changes of cochlear inner hair cells (IHCs) and outer hair cells (OHCs).


METHODS
IHCs and OHCs were acutely isolated from the guinea-pig cochlea. Cells were observed under the inverted microscope and 10mmol/L sodium salicylate solutions or 0.01mmol/L dexamethasone-plus-salicylate solutions were applied. The cell length or the ratio between the length and width was the indices of the morphological changes in cells.


RESULTS
Isolated IHCs did not demonstrate any significant changes in sodium salicylate solutions in 20min and in 40min, whereas OHCs were shortened by the 10mmol/L sodium salicylate to 83% in 20min and 75% in 40min. There were no significant differences between in the dexamethasone-plus-salicylate solutions and in the control solutions after 20min and 40min both in IHCs and OHCs.


CONCLUSIONS
Although salicylate affected the isolated OHCs from guinea-pig cochlea, IHCs were not changed morphologically by sodium salicylate applications. Dexamethasone inhibited the salicylate-induced morphological changes of OHCs.
Kimitsuki
Takashi
T
Department of Otolaryngology, Kyushu University, Higashi-ku, Fukuoka, Japan. kimitaka@qent.med.kyushu-u.ac.jp
Kakazu
Yasuhiro
Y
Matsumoto
Nozomu
N
Noda
Teppei
T
Komune
Noritaka
N
Komune
Shizuo
S
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000633:toxicity*; D048429:Cell Size / Q000187:drug effects; D003051:Cochlea / Q000187:drug effects / Q000473:pathology; D003907:Dexamethasone / Q000494:pharmacology; D006168:Guinea Pigs; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects* / Q000473:pathology; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects* / Q000473:pathology; D066298:In Vitro Techniques; D012980:Sodium Salicylate / Q000633:toxicity*
D000894:Anti-Inflammatory Agents, Non-Steroidal; D003907:Dexamethasone; D012980:Sodium Salicylate