title: [Biocompatibility of a novel biological piezoelectric ceramic to the rat periosteum derived osteoblast].
abstract: OBJECTIVE
  A novel biological piezoelectric ceramic was made by beta-tricalcium phosphate (beta-TCP) and lithium sodium potassium niobate (LNK) piezoelectric ceramics. To study its biocompatibility to osteoblast isolated from the cranium of 1-day-old Sprague-Dawley mice.
  
  
  METHODS
  The biological piezoelectric ceramic TCPLNK1/10, TCPLNK5/5 respectively mixed by beta-TCP and LNK piezoelectric ceramic at the ratio of 1/10 and 5/5. Then osteoblasts were used and seeded respectively on the negative and positive surfaces of TCPLNK1/10 and TCPLNK5/5. Growth and proliferation of the osteoblasts on TCPLNK1/10 and TCPLNK5/5 surfaces were evaluated in vitro by means of scanning electron microscopy (SEM) examination, fluorescence dyeing of osteoblast skeleton protein and MTT assay.
  
  
  RESULTS
  Cell morphology of osteoblast on positive and negative surfaces of TCPLNK1/10 and TCPLNK5/5 was normal, and both adhesion and growth characteristics showed better than control group. The growing osteoblasts on the TCPLNK1/10 negative surface were significantly higher than others. The negative surface of TCPLNK1/10 possessed better osteogenesis potential than others in vitro.
  
  
  CONCLUSIONS
  The surface of TCPLNK may permit the imitation piezoelectric effect of natural bone for bone regeneration.
authors.affiliation: Department of Stomatology, Affiliated Hospital of Hebei University, Baoding 071000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.forename: Xiao-Zhou
authors.initials: XZ
authors.lastname: Zhang
authors.forename: Ping
authors.initials: P
authors.lastname: Yang
authors.forename: Bao-Min
authors.initials: BM
authors.lastname: Tian
authors.forename: Zhi-Liang
authors.initials: ZL
authors.lastname: Liu
authors.forename: Zhi-Qing
authors.initials: ZQ
authors.lastname: Chen
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D001842:Bone and Bones; D002130:Calcium Phosphates; D049109:Cell Proliferation; D002516:Ceramics; D051379:Mice; D008855:Microscopy, Electron, Scanning; D009556:Niobium; D010006:Osteoblasts; D010012:Osteogenesis; D010087:Oxides; D010521:Periosteum; D051381:Rats; D017207:Rats, Sprague-Dawley; D012886:Skull
chemical_list: D002130:Calcium Phosphates; D010087:Oxides; C485817:beta-tricalcium phosphate; D009556:Niobium; C091692:lithium niobate