title: [Assessment of sensitivity of commercial test-systems for HBsAg immunodetection according to their ability to detect HBsAg-mutants of hepatitis B virus].
abstract: Comparative study of widely distributed on Russian market commercial test-systems for HBsAg detection by enzyme immunoassay was performed. Panel of serum samples containing mutant forms of HBsAg was developed for the study. It showed that only 2 out of 7 studied test-systems are able to detect mutant forms of HBsAg with the same sensitivity as "wild-type" forms of HBsAg. Test-systems based only on monoclonal antibodies did not detect HBsAg with G145R substitution in concentration 5 ng/ml. The study confirms the relevance of problem of HBsAg-mutants detection for hepatitis B immunodiagnostics.
authors.forename: A I
authors.initials: AI
authors.lastname: Bazhenov
authors.forename: M V
authors.initials: MV
authors.lastname: Konopleva
authors.forename: A A
authors.initials: AA
authors.lastname: Fel'dsherova
authors.forename: D A
authors.initials: DA
authors.lastname: El'gort
authors.forename: Iu S
authors.initials: IuS
authors.lastname: Khats
authors.forename: N E
authors.initials: NE
authors.lastname: Lapina
authors.forename: M A
authors.initials: MA
authors.lastname: Godkov
authors.forename: A P
authors.initials: AP
authors.lastname: Suslov
mesh_terms: D006509:Hepatitis B / Q000175:diagnosis* / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000097:blood* / Q000235:genetics; D006801:Humans; D007124:Immunoenzyme Techniques; D009154:Mutation; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic