A simple and rapid immunochromatographic strip test for detecting antibody to porcine reproductive and respiratory syndrome virus.
Porcine reproductive and respiratory syndrome is rapidly gaining worldwide importance as one of the most economically significant diseases of swine. The antibody of Porcine reproductive and respiratory syndrome virus (PRRSV) is detected currently by the combined use of an enzyme-linked immunosorbent assay, serum neutralization test, immunoperoxidase monolayer assay, indirect immunofluorescent antibody test. These methods are time-consuming and require specialized equipment operated by trained technicians. The purpose of this study was to evaluate a simple strip assay (based on a chromatographic and immunogold system) for specific detection of PRRSV antibody in swine sera. This "immunochromatographic strip" test uses Escherichia coli-expressed viral recombinant membrane protein antigen in combination with recombinant nucleocapsid protein as capture protein for detecting antibodies against PRRSV. In this study, the performance of this assay was evaluated with sera from both clinical samples and experimentally infected piglets. Detection by immunochromatographic strip test was compared with detection by a standard, available commercially, indirect enzyme-linked immunosorbent assay and an immunoperoxidase monolayer assay. The immunochromatographic test strip detected antibodies in sera known to contain antibodies to PRRSV in 95.7% sensitivity of samples from pigs infected experimentally and 98.6% sensitivity of clinical serum samples. For sera that did not contain antibodies to PRRSV, the specificity was 97.8% and 98.2% for clinical and experimental serum samples, respectively.
Cui
Shangjin
S
Division of Swine Infectious Disease, National Key Library of Veterinary Biotechnology, Harbin Veterinary Research Institute of CAAS, Nangang District, Harbin, Heilongjiang, People's Republic of China. cuishangjin@yahoo.com.cn
Zhou
Shenghua
S
Chen
Changmu
C
Qi
Ting
T
Zhang
Chaofan
C
Oh
JinSik
J
D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D007124:Immunoenzyme Techniques / Q000379:methods; D019590:Nucleocapsid Proteins / Q000276:immunology; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology* / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000276:immunology*; D011934:Reagent Strips; D011994:Recombinant Proteins / Q000276:immunology*; D012680:Sensitivity and Specificity; D013552:Swine
D000914:Antibodies, Viral; D019590:Nucleocapsid Proteins; D011934:Reagent Strips; D011994:Recombinant Proteins