title: Contrasting human cytokine responses to promastigote whole-cell extract and the Leishmania analogue receptor for activated C kinase antigen of L. amazonensis in natural infection versus immunization.
abstract: It is known that the same antigen can induce different immune responses, depending upon the way that it is presented to the immune system. The objective of this study was to compare cytokine responses of peripheral blood mononuclear cells (PBMC) from cutaneous leishmaniasis patients and subjects immunized with a first-generation candidate vaccine composed of killed Leishmania amazonensis promastigotes to a whole-cell promastigote antigen extract (La) and to the recombinant protein LACK (Leishmania analogue receptor for activated C kinase), both from L. amazonensis. Thirty-two patients, 35 vaccinees and 13 healthy subjects without exposure to Leishmania, were studied. Cytokine production was assessed by enzyme-linked immunosorbent assay and enzyme-linked immunospot assay. The interferon (IFN)-gamma levels stimulated by La were significantly higher and the levels of interleukin (IL)-10 significantly lower than those stimulated by LACK in the patient group, while LACK induced a significantly higher IFN-gamma production and a significantly lower IL-10 production compared with those induced by La in the vaccinated group. LACK also induced a significantly higher frequency of IFN-gamma-producing cells than did La in the vaccinated group. The contrast in the cytokine responses stimulated by LACK and La in PBMC cultures from vaccinated subjects versus patients indicates that the human immune response to crude and defined Leishmania antigens as a consequence of immunization differs from that induced by natural infection.
authors.affiliation: Laboratório de Imunoparasitologia, Instituto Oswaldo Cruz, Fiocruz, Manguinhos, Rio de Janeiro, Brazil.
authors.forename: R B G
authors.initials: RB
authors.lastname: Azeredo-Coutinho
authors.forename: D C S
authors.initials: DC
authors.lastname: Matos
authors.forename: G G R
authors.initials: GG
authors.lastname: Armôa
authors.forename: R M
authors.initials: RM
authors.lastname: Maia
authors.forename: A
authors.initials: A
authors.lastname: Schubach
authors.forename: W
authors.initials: W
authors.lastname: Mayrink
authors.forename: S C F
authors.initials: SC
authors.lastname: Mendonça
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000818:Animals; D000953:Antigens, Protozoan / Q000276:immunology*; D001938:Brazil; D016022:Case-Control Studies; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology*; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D007891:Leishmania / Q000276:immunology*; D054332:Leishmaniasis Vaccines / Q000276:immunology*; D016773:Leishmaniasis, Cutaneous / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000953:Antigens, Protozoan; D016207:Cytokines; D054332:Leishmaniasis Vaccines; D016753:Interleukin-10; D007371:Interferon-gamma