title: [Effects of activated PPARgamma on the expression of PTEN gene in pancreatic cancer cells].
abstract: OBJECTIVE
  To explore the relationship between the expression of PTEN gene and the expression of PPARgamma, and the human pancreatic cancer cells PANC-1 were cultured in vitro.
  
  
  METHODS
  The effects of rosiglitazone and GW9662 on the expression of PTEN gene and PTEN protein in the human pancreatic cancer cells PANC-1 were detected by RT-PCR and immunohistochemistry respectively. In addition, the percentage of the expression of PTEN protein was analyzed by flow cytometry.
  
  
  RESULTS
  The expression of PTEN gene and PTEN protein in human pancreatic cancer cells PANC-1 were all increased significantly after treated with rosiglitazone. While those were markedly reduced in GW9662 treated groups, and it has a dose-effect relationship between them.
  
  
  CONCLUSIONS
  The expression of PTEN gene were paralleled with the expression of PPARgammain human pancreatic cancer cells PANC-1, which may be related to its inhibitory effects on pancreatic tumor cells.
authors.affiliation: Department of Oncology, the Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China. baimh2004@126.com
authors.forename: Ming-Hua
authors.initials: MH
authors.lastname: Bai
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Ma
authors.forename: Xi-Jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Xiao-Xu
authors.initials: XX
authors.lastname: Liu
authors.forename: Xing-Huan
authors.initials: XH
authors.lastname: Xue
authors.forename: Feng-Jie
authors.initials: FJ
authors.lastname: Xue
mesh_terms: D000813:Anilides / Q000494:pharmacology; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D007150:Immunohistochemistry; D047495:PPAR gamma / Q000037:antagonists & inhibitors / Q000378:metabolism*; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism*; D010190:Pancreatic Neoplasms / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D047495:PPAR gamma; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D051059:PTEN Phosphohydrolase