title: Extracellular matrix of the regeneration chamber and plasma membranes of the epidermis during leg regeneration in an insect Carausius morosus.
abstract: An extracellular matrix (ECM) is found in the regeneration chamber during leg regeneration in the stick insect Carausius morosus. The material which surrounds the regenerate is organised into fibrils and it includes proteins distributed in a hydrated polysaccharide gel. The compounds which can be demonstrated are chitin unlinked to proteins, glycoproteins and unsulfated glycosaminoglycans such as hyaluronic acid and/or chondroitin. Molecules related to vertebrate fibronectin and collagen IV were observed on the apical surface of the epidermal cells of the regenerate. During leg regeneration, the basal lamina which normally secures the cells to each other is absent. However a condensation of material on the regenerate epidermal cells ensures their cohesion. The extracellular matrix in the regeneration chamber must be secreted by the cells of retracted epidermis and then by the epidermal cells of the regenerate, until these cells are able to secrete the cuticle for the next instar. The analysis of the epidermal cell surface does not seem to show any localization or any changes during the development of the regenerate.
authors.affiliation: Laboratoire de Biologie du Développement, Université des Sciences et Techniqes de Lille Flandres Artois, 59655 Villeneuve d'Ascq Cedex, France.
authors.forename: E
authors.initials: E
authors.lastname: Browaeys-Poly