Cucumber mosaic virus-associated RNA 5: X. The complete nucleotide sequence of a CARNA 5 incapable of inducing tomato necrosis.
The complete nucleotide sequence of a cucumber mosaic virus-associated RNA 5 (CARNA 5) that does not induce the tomato necrosis disease (J. M. Kaper, M. E. Tousignant, and S. M. Thompson, Virology 114, 526-533, 1981) has been determined and compared with the known sequence of a necrosis-inducing CARNA 5 (K. E. Richards, G. Jonard, M. Jacquemond, and H. Lot, Virology 89, 395-408, 1978). The nonnecrotic satellite RNA, (1)CARNA 5, is one nucleotide smaller yet 93% homologous with (n)CARNA 5, the necrosis-inducing satellite RNA. (1)CARNA 5 differs from (n)CARNA 5 by 21 substitutions, 3 deletions, and 2 insertions, with more changes in the 3' half than in the 5' half of the molecule. (1)CARNA 5, like (n)CARNA 5, is capped at its 5' terminus; the nucleotide sequences predict that putative translational products of the two RNAs could be significantly different.
Whitmer Collmer
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Department of Chemistry, University of Maryland, College Park, Maryland 20742, USA.
Tousignant
M E
ME
Kaper
J M
JM