Integrins mediate adherence and migration of T lymphocytes on human peritoneal mesothelial cells.
We previously showed that a local immune response largely composed of type 1 T cells correlated with a favorable outcome of the peritonitis associated with peritoneal dialysis. To clarify how these subsets are recruited to the peritoneal cavity during inflammation, we measured integrin-mediated interactions between the T cells and human peritoneal mesothelial cells. Direct microscopy showed that lipopolysaccharide or peritoneal dialysis effluent stimulated the adherence of T cells to mesothelial cells, a process mediated by the integrins alpha6beta1 and alpha4beta1. Further, the migration of Th1 cell across human mesothelial cell monolayers grown on transwell surfaces was reduced by anti-alpha6beta1 integrin antibody while that of Th2 cell was inhibited by an anti-alpha4 integrin antibody. Pretreatment with either lipopolysaccharide or rapid response peritoneal dialysis effluent stimulated T cell migration and this was significantly decreased by the alpha6beta1 compared to the alpha4 antibody. These results suggest that integrins may play an important role in mediating selective T cell subset adhesion and migration across human peritoneal mesothelial cell monolayers and differential integrin expression and selective T cell subset recruitment during peritonitis may affect outcome.
Wang
Hsin-Hui
HH
Department of Pediatrics, Section of Nephrology, Taipei Veterans General Hospital, Taipei, Taiwan.
Lee
Tzong-Yann
TY
Lin
Ching-Yuang
CY
D001202:Ascitic Fluid; D002448:Cell Adhesion; D002634:Chemotaxis, Leukocyte; D004847:Epithelial Cells / Q000473:pathology; D006801:Humans; D039041:Integrin alpha4beta1 / Q000378:metabolism* / Q000502:physiology; D039121:Integrin alpha6beta1 / Q000378:metabolism* / Q000502:physiology; D016023:Integrins / Q000378:metabolism* / Q000502:physiology; D008070:Lipopolysaccharides / Q000494:pharmacology; D010529:Peritoneal Cavity / Q000473:pathology; D010537:Peritoneum / Q000473:pathology*; D010538:Peritonitis / Q000276:immunology*; D013601:T-Lymphocytes / Q000502:physiology*; D018417:Th1 Cells / Q000502:physiology; D018418:Th2 Cells / Q000502:physiology
D039041:Integrin alpha4beta1; D039121:Integrin alpha6beta1; D016023:Integrins; D008070:Lipopolysaccharides