title: Abdominal adipose tissue: early metabolic dysfunction associated to insulin resistance and oxidative stress induced by an unbalanced diet.
abstract: The possible contribution of early changes in lipid composition, function, and antioxidant status of abdominal adipose tissue (AAT) induced by a fructose-rich diet (FRD) to the development of insulin resistance (IR) and oxidative stress (OS) was studied. Wistar rats were fed with a commercial diet with (FRD) or without 10% fructose in the drinking water for 3 weeks. The glucose (G), triglyceride (TG), and insulin (I) plasma levels, and the activity of antioxidant enzymes, lyposoluble antioxidants, total glutathione (GSH), lipid peroxidation as TBARS, fatty acid (FA) composition of AAT-TG as well as their release by incubated pieces of AAT were measured. Rats fed with a FRD have significantly higher plasma levels of G, TG, and I. Their AAT showed a marked increase in content and ratios of saturated to monounsaturated and polyunsaturated FAs, TBARS, and catalase, GSH-transferase and GSH-reductase, together with a decrease in superoxide dismutase and GSH-peroxidase activity, and total GSH, alpha-tocopherol, beta-carotene and lycopene content. Incubated AAT from FRD released in vitro higher amount of free fatty acids (FFAs) with higher ratios of saturated to monounsaturated and polyunsaturated FAs. Our data suggest that FRD induced an early prooxidative state and metabolic dysfunction in AAT that would favor the overall development of IR and OS and further development of pancreatic beta-cell failure; therefore, its early control would represent an appropriate strategy to prevent alterations such as the development of type 2 diabetes.
authors.affiliation: CENEXA-Center of Experimental and Applied Endocrinology (National University of La Plata-National Research Council, PAHO/WHO Collaborating Center), 60 y 120 La Plata, Argentina. oscar_rebolledo@yahoo.com.ar
authors.forename: O R
authors.initials: OR
authors.lastname: Rebolledo
authors.forename: C A
authors.initials: CA
authors.lastname: Marra
authors.forename: A
authors.initials: A
authors.lastname: Raschia
authors.forename: S
authors.initials: S
authors.lastname: Rodriguez
authors.forename: J J
authors.initials: JJ
authors.lastname: Gagliardino
mesh_terms: D050153:Abdominal Fat / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D000818:Animals; D000975:Antioxidants / Q000032:analysis; D001786:Blood Glucose / Q000032:analysis; D004032:Diet; D004040:Dietary Carbohydrates / Q000008:administration & dosage; D005227:Fatty Acids / Q000032:analysis; D005230:Fatty Acids, Nonesterified / Q000378:metabolism; D005632:Fructose / Q000008:administration & dosage; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D018384:Oxidative Stress / Q000502:physiology*; D051381:Rats; D017208:Rats, Wistar; D017392:Thiobarbituric Acid Reactive Substances / Q000032:analysis; D014280:Triglycerides / Q000097:blood
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D004040:Dietary Carbohydrates; D005227:Fatty Acids; D005230:Fatty Acids, Nonesterified; D007328:Insulin; D017392:Thiobarbituric Acid Reactive Substances; D014280:Triglycerides; D005632:Fructose