title: Evaluation of three real-time PCR assays for detection of Mycoplasma pneumoniae in an outbreak investigation.
abstract: We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.
authors.affiliation: Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA. jwinchell@cdc.gov
authors.forename: Jonas M
authors.initials: JM
authors.lastname: Winchell
authors.forename: Kathleen A
authors.initials: KA
authors.lastname: Thurman
authors.forename: Stephanie L
authors.initials: SL
authors.lastname: Mitchell
authors.forename: W Lanier
authors.initials: WL
authors.lastname: Thacker
authors.forename: Barry S
authors.initials: BS
authors.lastname: Fields
mesh_terms: D000293:Adolescent; D000328:Adult; D015342:DNA Probes; D004196:Disease Outbreaks; D006801:Humans; D008969:Molecular Sequence Data; D009177:Mycoplasma pneumoniae; D011019:Pneumonia, Mycoplasma / Q000175:diagnosis* / Q000382:microbiology; D016133:Polymerase Chain Reaction; D055815:Young Adult
chemical_list: D015342:DNA Probes