title: Microencapsulation selection for isolation of yeast mutants with increased secretion of Aspergillus awamori glucoamylase.
abstract: We have developed a microencapsulation selection method which allows the rapid and quantitative screening of >10(6) yeast cells for enhanced secretion of Aspergillus awamori glucoamylase. The method provides a 400-fold single-pass enrichment for high-secreting mutants, and can be straightforwardly adapted for application to growth-based selection schemes with other microorganisms and enzymes.
authors.affiliation: Department of Chemical Engineering, University of Illinois, Urbana, Illinois 61801, USA.
authors.forename: K D
authors.initials: KD
authors.lastname: Wittrup
authors.forename: A L
authors.initials: AL
authors.lastname: Weber
authors.forename: P
authors.initials: P
authors.lastname: Tsai