title: Structured illumination in total internal reflection fluorescence microscopy using a spatial light modulator.
abstract: In wide-field fluorescence microscopy, illuminating the specimen with evanescent standing waves increases lateral resolution more than twofold. We report a versatile setup for standing-wave illumination in total internal reflection fluorescence microscopy. An adjustable diffraction grating written on a phase-only spatial light modulator controls the illumination field. Selecting appropriate diffraction orders and displaying a sheared (tilted) diffraction grating allows one to tune the penetration depth in very fine steps. The setup achieves 91 nm lateral resolution for green emission.
authors.affiliation: Department of Mechanical and Process Engineering, ETH Zurich, Zurich, Switzerland.
authors.forename: Reto
authors.initials: R
authors.lastname: Fiolka
authors.forename: Markus
authors.initials: M
authors.lastname: Beck
authors.forename: Andreas
authors.initials: A
authors.lastname: Stemmer