Real-time PCR and statistical analyses of acrAB and ramA expression in clinical isolates of Klebsiella pneumoniae.
Clinical isolates of Klebsiella pneumoniae were tested for a correlation between tigecycline MIC and expression of ramA by using real-time PCR. At MICs of 4 and 8 microg/ml, the expression of ramA was statistically significantly different from MICs of 2 microg/ml or less, supporting the tigecycline susceptibility breakpoint of <or=2 microg/ml for K. pneumoniae.
Ruzin
Alexey
A
Department of Infectious Disease, Wyeth Research, Pearl River, New York 10965, USA. ruzina@wyeth.com
Immermann
Frederick W
FW
Bradford
Patricia A
PA
D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D006801:Humans; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D008826:Microbial Sensitivity Tests / Q000592:standards; D008911:Minocycline / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D016133:Polymerase Chain Reaction / Q000379:methods*; D000078304:Tigecycline
D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D026901:Membrane Transport Proteins; C095947:RamA protein, Klebsiella; D000078304:Tigecycline; D008911:Minocycline