Isolation, characterization and quantitation of canine alpha-1-acid glycoprotein.
The present study was designed to investigate the physicochemical properties of canine alpha-1-acid glycoprotein (AGP), and to establish a method for measuring serum AGP in canine. Fifty-three normal beagles were used in the study. AGP was purified from normal canine sera by successive ammonium sulfate precipitation, ion-exchange chromatography and gel filtration. The serum AGP concentration was measured by single radial immunodiffusion (SRID). Canine AGP had a molecular weight of 42,000 +/- 2,000 Da and contained 40.9% carbohydrate. Gel isoelectric focusing revealed microheterogeneity with 6-7 bands in a pI range (isoelectric points) of 3.2-3.8. AGP migrated to the alpha1-globulin region on immunoelectrophoresis. The serum AGP level of 35 normal beagles was 283.4 +/- 113.3 microg/ml. Canine AGP was isolated, and its physicochemical properties were clarified. SRID may be a useful method for quantification of serum AGP in canine.
Yuki
M
M
Yuki Animal Hospital, 2-99 Kiba-cho, Minato-ku, Nagoya, Aichi, 455-0021, Japan.
Itoh
H
H
Tamura
K
K
Nishii
N
N
Takase
K
K
D000818:Animals; D004285:Dogs / Q000097:blood*; D009961:Orosomucoid / Q000737:chemistry* / Q000235:genetics*; D012698:Serologic Tests / Q000379:methods / Q000662:veterinary
D009961:Orosomucoid