Gold nanoparticle-based surface enhanced Raman scattering spectroscopic assay for the detection of protein-protein interactions.
In the present work, a sensitive spectroscopic assay based on surface-enhanced Raman spectroscopy (SERS) using gold nanoparticles as substrates was developed for the rapid detection protein-protein interactions. Detection is achieved by specific binding biotin-modification antibodies with protein-stabilized 30 nm gold nanoparticles, followed by the attachment of avidin-modification Raman-active dyes. As a proof-of-principle experiment, a well-known biomolecular recognition system, IgG with protein A, was chosen to establish this new spectroscopic assay. Highly selective recognition of IgG down to 1 ng/ml in solution has been demonstrated.
Li
Tao
T
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China.
Guo
Liping
L
Wang
Zhenxin
Z
D000911:Antibodies, Monoclonal / Q000276:immunology; D001360:Avidin / Q000276:immunology / Q000378:metabolism; D001665:Binding Sites; D001710:Biotin / Q000276:immunology / Q000378:metabolism; D006046:Gold / Q000737:chemistry*; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism*; D053768:Metal Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation / Q000379:methods*; D010316:Particle Size; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D013059:Spectrum Analysis, Raman / Q000295:instrumentation / Q000379:methods*; D013205:Staphylococcal Protein A / Q000276:immunology / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D007074:Immunoglobulin G; D013205:Staphylococcal Protein A; D001360:Avidin; D001710:Biotin; D006046:Gold