[Odontoblast-like cell phenotype differentiation of cranial neural crest stem cell in vivo].
OBJECTIVE
To investigate the feasibility of tooth regeneration by seeding cranial neural crest stem cell (CNCSC) in vivo.


METHODS
Cranial neural tubes, dissected from mouse E9 d, were explanted onto fibronectin-coated dishes. CNCSC emigrated from the explanted neural tubes, and were cultured in a free-serum medium containing modified DMEM/F12. CNCSC, induced by FGF8, BMP2, TGFbeta1 and dentin matrix non-collagen protein (DMNCP), were cultured with collagen/chitosan, and implanted into the subcutaneous part of immunodeficiency mouse. The expression of collagen I/dentin sialophosphoprotein (DSPP) was analyzed by immunocytochemistry.


RESULTS
With the scaffolds destroying, columnar cells possessing polarized nuclei and matrix produced by cells were showed in some regions. Immunohistochemical staining demonstrated that collagen type I and DSPP were expressed throughout the cytoplasm and matrix produced by cells.


CONCLUSIONS
By tissue engineering approach, our experiments further verify the odontoblast-like cell phenotype differentiation of CNCSC in vivo.
Jiang
Hong-Bing
HB
State Key Laboratory of Oral Diseases, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Tian
Wei-Dong
WD
Liu
Lei
L
Tang
Wei
W
Zheng
Xiao-Hui
XH
Chen
Xi-Zhe
XZ
D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis; D003391:Cranial Nerves / Q000737:chemistry / Q000166:cytology; D016326:Extracellular Matrix Proteins; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009432:Neural Crest / Q000737:chemistry / Q000166:cytology*; D009804:Odontoblasts / Q000737:chemistry / Q000166:cytology*; D010750:Phosphoproteins; D019736:Prostheses and Implants; D011498:Protein Precursors / Q000032:analysis; D012038:Regeneration; D012795:Sialoglycoproteins; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*; D014070:Tooth / Q000737:chemistry / Q000254:growth & development / Q000502:physiology; D000099311:Dentin Sialophosphoprotein
D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein