[Estimation of selected parameters antioxidant barrier and lipid parameters in children with atherosclerosis risk factors].
Atherosclerosis is one of the most common causes of mortality in developed countries. One of the many factors in its etiopathogenesis are reactive oxygen species (ROS), excess of which comes from disturbances of enzymatic and nonenzymatic mechanisms of antioxidant barrier. THE AIM OF THIS STUDY was to estimate relations between the activity of chosen parameters antioxidant barrier and the lipid levels in children with atherosclerosis risk factors.


METHODS
The study was carried out on 48 children with atherosclerosis risk factors. The control group consisted of 25 healthy children. Total cholesterol (TC), Triglycerides (TG), HDL-C, LDL-C were determined by enzymatic method. The activity of superoxide dismutase (SOD) was estimated by Misra and Fridivich's method, glutathione peroxidase (GSH-Px) with method proposed by Sedlak and Lindsay in modyfication Little and Brien and the activity o catalase (CAT) by Beer and Sizer's method. Malonyl dialdehyde (MDA) concentration was determined by Placer's method.


RESULTS
Obesity, lipid disorders, and hypertension were the most frequent risk factors in the investigated children. Statistically significant higher concentration of TC, LDL-C, TG and lower HDL-C were observed in children with atherosclerosis risk factors. Activity of glutathion peroxidase was statistically significant higher in children with atherosclerosis risk factors (p < 0.001), activity of catalase was lower (p < 0.05). No significant differences in activity of superoxide dismutase and malonyl dialdehyde were observed in the investigated groups.


CONCLUSIONS
Modificated activity of selected antioxidants in children with atherosclerosis risk factors could determine an index of antioxidant barrier and might potencially constitute the marker of early atherosclerotical risk development.
Sierakowska-Fijałek
Anna
A
Uniwersytet Medyczny w Łodzi, II Zakład Medycyny Rodzinnej. annasierakowska@poczta.onet.pl
Fijałkowski
Paweł
P
Błaszczyk
Jan
J
Baj
Zbigniew
Z
Stepień
Mariusz
M
Wosik-Erenbek
Marzenna
M
Rysz
Jacek
J
D000293:Adolescent; D000975:Antioxidants / Q000378:metabolism*; D050197:Atherosclerosis / Q000175:diagnosis* / Q000453:epidemiology / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D015984:Causality; D002784:Cholesterol / Q000097:blood; D008076:Cholesterol, HDL / Q000097:blood; D008078:Cholesterol, LDL / Q000097:blood; D015897:Comorbidity; D050171:Dyslipidemias / Q000453:epidemiology; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D006801:Humans; D006973:Hypertension / Q000453:epidemiology; D050356:Lipid Metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D009765:Obesity / Q000453:epidemiology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012307:Risk Factors; D013482:Superoxide Dismutase / Q000378:metabolism; D014280:Triglycerides / Q000097:blood
D000975:Antioxidants; D015415:Biomarkers; D008076:Cholesterol, HDL; D008078:Cholesterol, LDL; D017382:Reactive Oxygen Species; D014280:Triglycerides; D008315:Malondialdehyde; D002784:Cholesterol; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase