title: Bacteriophage P22 and Staphylococcus aureus attenuation on nonporous fomites as determined by plate assay and quantitative PCR.
abstract: Decay rates of bacteriophage P22 and Staphylococcus aureus on six types of common household inanimate surfaces were evaluated based on cultivation and quantitative PCR. A much higher level of inactivation was observed using the plate assay, suggesting that detection of the pathogen genome in samples from fomites does not necessarily imply a health risk to humans.
authors.affiliation: Tohoku University, Department of Civil and Environmental Engineering, 6-6-06 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan. masago@water.civil.tohoku.ac.jp
authors.forename: Yoshifumi
authors.initials: Y
authors.lastname: Masago
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Shibata
authors.forename: Joan B
authors.initials: JB
authors.lastname: Rose
mesh_terms: D017100:Bacteriophage P22 / Q000235:genetics / Q000254:growth & development*; D015169:Colony Count, Microbial; D004269:DNA, Bacterial / Q000032:analysis; D004279:DNA, Viral / Q000032:analysis; D003890:Desiccation; D050456:Fomites / Q000382:microbiology*; D006801:Humans; D050296:Microbial Viability; D016133:Polymerase Chain Reaction; D016062:Porosity; D018570:Risk Assessment; D013211:Staphylococcus aureus / Q000235:genetics / Q000254:growth & development*
chemical_list: D004269:DNA, Bacterial; D004279:DNA, Viral