Cauliflower mosaic virus-induced viroplasms support viral DNA synthesis in a cell-free system.
Viroplasms are the main cytological modifications observed upon infection of Brassica cells by cauliflower mosaic virus (CaMV). Previous experiments suggested that the replication of viral DNA proceeded in two steps, starting in the nucleus and going on to the viroplasms. Recent evidence has been obtained on the role of the nuclear step of CaMV DNA replication. We have developed an in vitro system, derived from infected leaves, which is able to synthesize viral DNA and which contains nuclei and viroplasms, the putative sites of CaMV replication. In such a system, viroplasms are the sites of active DNA synthesis, and replicated viral DNA molecules are preferentially associated with them.
Modjtahedi
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N
Section de Biologie, Institut Curie, 26 rue d'Ulm, 75231 Paris Cx 05, France.
Volovitch
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M
Sossountzov
L
L
Habricot
Y
Y
Bonneville
J M
JM
Yot
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P