title: Distinct molecular pathways for development of telencephalic interneuron subtypes revealed through analysis of Lhx6 mutants.
abstract: Here we analyze the role of the Lhx6 lim-homeobox transcription factor in regulating the development of subsets of neocortical, hippocampal, and striatal interneurons. An Lhx6 loss-of-function allele, which expresses placental alkaline phosphatase (PLAP), allowed analysis of the development and fate of Lhx6-expressing interneurons in mice lacking this homeobox transcription factor. There are Lhx6+;Dlx+ and Lhx6-;Dlx+ subtypes of tangentially migrating interneurons. Most interneurons in Lhx6(PLAP/PLAP) mutants migrate to the cortex, although less efficiently, and exhibit defects in populating the marginal zone and superficial parts of the neocortical plate. By contrast, migration to superficial parts of the hippocampus is not seriously affected. Furthermore, whereas parvalbumin+ and somatostatin+ interneurons do not differentiate, NPY+ interneurons are present; we suggest that these NPY+ interneurons are derived from the Lhx6-;Dlx+ subtype. Striatal interneurons show deficits distinct from pallial interneurons, including a reduction in the NPY+ subtype. We provide evidence that Lhx6 mediates these effects through promoting expression of receptors that regulate interneuron migration (ErbB4, CXCR4, and CXCR7), and through promoting the expression of transcription factors either known (Arx) or implicated (bMaf, Cux2, and NPAS1) in controlling interneuron development.
authors.affiliation: Laboratory of Mammalian Genes and Development, National Institute of Child Health and Human Development, Bethesda, Maryland 20892, USA.
authors.forename: Yangu
authors.initials: Y
authors.lastname: Zhao
authors.forename: Pierre
authors.initials: P
authors.lastname: Flandin
authors.forename: Jason E
authors.initials: JE
authors.lastname: Long
authors.forename: Melissa Dela
authors.initials: MD
authors.lastname: Cuesta
authors.forename: Heiner
authors.initials: H
authors.lastname: Westphal
authors.forename: John L R
authors.initials: JL
authors.lastname: Rubenstein
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000502:physiology*; D002465:Cell Movement; D002540:Cerebral Cortex / Q000166:cytology; D003342:Corpus Striatum / Q000166:cytology / Q000378:metabolism; D004622:Embryo, Mammalian; D006624:Hippocampus / Q000166:cytology / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism*; D007395:Interneurons / Q000166:cytology* / Q000378:metabolism / Q000502:physiology; D060850:LIM-Homeodomain Proteins; D051379:Mice / Q000196:embryology / Q000254:growth & development; D008815:Mice, Inbred Strains; D009154:Mutation; D019579:Neocortex / Q000166:cytology / Q000378:metabolism; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism*; D013687:Telencephalon / Q000166:cytology* / Q000378:metabolism*; D014157:Transcription Factors
chemical_list: D018398:Homeodomain Proteins; C498504:LHX6 protein, mouse; D060850:LIM-Homeodomain Proteins; D009419:Nerve Tissue Proteins; D014157:Transcription Factors