title: Ototoxic interaction of kanamycin and 3-nitropropionic acid.
abstract: CONCLUSIONS
  Mitochondrial dysfunction in the cochlea potentiates the ototoxicity of aminoglycosides.
  
  
  OBJECTIVE
  This study examined whether mitochondrial dysfunction in the cochlea affects the ototoxicity of aminoglycosides.
  
  
  METHODS
  Nineteen guinea pigs were treated with the mitochondrial toxin 3-nitropropionic acid (3-NP), kanamycin, both agents, or normal saline as control. After 14 days, hair cell loss and auditory brainstem response (ABR) were assessed.
  
  
  RESULTS
  The administration of 400 mg/kg of kanamycin caused neither hair cell loss nor ABR threshold shift. Administration of 3-NP caused mild ABR threshold shift without significant hair cell loss. Administration of 3-NP and kanamycin caused ABR threshold shift and significant hair cell loss.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Chia-Der
authors.initials: CD
authors.lastname: Lin
authors.forename: Takeshi
authors.initials: T
authors.lastname: Oshima
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Oda
authors.forename: Daisuke
authors.initials: D
authors.lastname: Yamauchi
authors.forename: Ming-Hsui
authors.initials: MH
authors.lastname: Tsai
authors.forename: Toshimitsu
authors.initials: T
authors.lastname: Kobayashi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001309:Auditory Threshold / Q000187:drug effects; D003292:Convulsants / Q000633:toxicity*; D004347:Drug Interactions; D016057:Evoked Potentials, Auditory, Brain Stem; D006168:Guinea Pigs; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects* / Q000473:pathology; D007612:Kanamycin / Q000633:toxicity*; D008297:Male; D008928:Mitochondria / Q000187:drug effects*; D009574:Nitro Compounds / Q000633:toxicity*; D011422:Propionates / Q000633:toxicity*
chemical_list: D000900:Anti-Bacterial Agents; D003292:Convulsants; D009574:Nitro Compounds; D011422:Propionates; D007612:Kanamycin; C015392:3-nitropropionic acid