SPE-HPTLC of procyanidins from the barks of different species and clones of Salix.
A SPE-HPTLC method was developed for the qualitative and quantitative analysis of procyanidin B(1) in willow barks. The chromatography was performed on HPTLC silica gel layer with the mobile phase chloroform-ethanol-formic acid (50:40:6 v/v/v), in the Automatic Developing Chamber-ADC 2. The methanol extracts from willow barks were purified by SPE method on RP-18 silica gel columns with methanol-water (7:93 v/v) as the eluent. The presence of procyanidin B(1) was revealed in the majority of investigated willow barks. The content of procyanidin B(1) varied from 0.26 mg/g in the extract of Salix purpurea clone 1067-2.24 mg/g in the extract of Salix alba clone 1100. The method was validated for linearity, precision, LOD, LOQ and repeatability.
Pobłocka-Olech
Loretta
L
Department of Pharmacognosy, Medical University of Gdańsk, Gen. J. Hallera 107 str., 80-416 Gdańsk, Poland.
Krauze-Baranowska
Mirosława
M
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002855:Chromatography, Thin Layer / Q000379:methods*; D002999:Clone Cells / Q000737:chemistry; D005782:Gels / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D015394:Molecular Structure; D024301:Plant Bark / Q000737:chemistry*; D010936:Plant Extracts / Q000032:analysis / Q000737:chemistry; D044945:Proanthocyanidins / Q000032:analysis*; D012015:Reference Standards; D015203:Reproducibility of Results; D032108:Salix / Q000737:chemistry* / Q000166:cytology / Q000235:genetics; D012822:Silicon Dioxide / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D013045:Species Specificity
D005782:Gels; D010936:Plant Extracts; D044945:Proanthocyanidins; D012997:Solvents; D012822:Silicon Dioxide; D000432:Methanol