[Ribosomal DNA ITS sequence of analysis of Psammosilene tunicoides from different populations].
OBJECTIVE
To analyze the ribosomal ITS sequence variation of Psammosilene tuncolides W. C. Wu et C. Y. Wu from different populations, for identifying different local populations.


METHODS
A pair of primers of 18SP1 and 26SP2 with PCR technique had been applied to study the ITS sequences.


RESULTS
The sequences of ITS1, ITS2 and 5.8S are 225-229 bp, 166-170 bp and 261-264 bp. Among 12 local populations, the sequence of Kunming, Lijiang, Gejiu, Heqing of Yunnan and Yanyuan of Sichuan showed no variation, there were 1-4 variable sites (including 5.8S coding region) in pairwise comparison of the other 7 local populations, including Xuanwei, Huize, Zhongdian, Baoshan of Yunnan, Muli of Sichuan and Linzhi of Tibet.


CONCLUSIONS
Comparative analysis shows that the ITS sequences of different local populations in the middle of Yunnan, southwest of Sichuan and west, northwest of Yunnan, southeast of Tibet, southwest of Sichuan have different fingerprint character, so the ITS sequences can be used to identify different local populations. The variation of ITS sequence of Psammosilene tunicoides is related to its geographical distribution.
Liu
Wen-Zhi
WZ
Yunan College of Traditional Chinese Medicine, Kunming 650200, China.
Dai
Zhu-Bo
ZB
Qian
Zi-Gang
ZG
D001483:Base Sequence; D029748:Caryophyllaceae / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D002681:China; D018744:DNA, Plant / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D005843:Geography; D008969:Molecular Sequence Data; D010802:Phylogeny; D010946:Plants, Medicinal / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D012340:RNA, Ribosomal, 5.8S / Q000235:genetics*; D017422:Sequence Analysis, DNA
D018744:DNA, Plant; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S