[The plasma level of sL-selectin, myeloperoxidase and granulocyte-colony stimulating factor (G-CSF) in breast cancer patients in the course of chemotherapy].
Neutrophils kill tumor cells through mechanisms of cell cytotoxicity, dependent on myeloperoxidase. The proliferation and activity of mature granulocytes are stimulated by the granulocyte--colony stimulating factor (G-CSF). The purpose of this investigation was to evaluate the level of adhesion molecule--sL-selectin, as a measure of mature granulocyte activity and myeloperoxidase responsible for their cytotoxicity. Additionally we have investigated the plasma level of G-CSF which can increase in the breast cancer. We tested 15 patients with III stage breast cancer and 18 patients with IV stage. Plasma samples were drawn before and in the course (12th week) of chemotherapy. The control group consisted of 10 healthy women. The plasma levels of sL-selectin, myeloperoxidase and G-CSF were measured using a sensitive sandwich ELISA system. In breast cancer patients (III and IV stage) before and in the course of chemotherapy sL-selectin concentration was decreased, but myeloperoxidase concentration increased in comparison to the control group. The level of sL-selectin and myeloperoxidase in the 12th week was decreased when compared to the level before chemotherapy. These results suggest that levels of tested parameters do not correlate with G-CSF concentration.
Czygier
Małgorzata
M
Zakład Diagnostyki Biochemicznej, Akademii Medycznej w Białymstoku. zdb@amb.edu.pl
Ławicki
Sławomir
S
Uścinowicz
Aniela
A
Szmitkowski
Maciej
M
D000328:Adult; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000473:pathology; D016022:Case-Control Studies; D005260:Female; D016179:Granulocyte Colony-Stimulating Factor / Q000097:blood*; D006098:Granulocytes / Q000201:enzymology / Q000378:metabolism*; D006801:Humans; D019041:L-Selectin / Q000097:blood*; D008875:Middle Aged; D009367:Neoplasm Staging; D009195:Peroxidase / Q000097:blood*
D019041:L-Selectin; D016179:Granulocyte Colony-Stimulating Factor; D009195:Peroxidase