Acrylamide: a dietary carcinogen formed in vivo?
Acrylamide, a chemical formed during heating of human foods, reacts with N-terminal valine in hemoglobin (Hb) and forms stable reaction products (adducts). These adducts to N-terminal valine in Hb have been used to estimate daily intake of acrylamide. Daily intake of acrylamide estimated from Hb adduct levels was higher than daily intake estimated from dietary questionnaires, possibly indicating other sources of exposures. Therefore, in this study the possible endogenous formation of acrylamide was investigated by treating mice with FeSO 4, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-hydrochloric acid (MPTP), or methamphetamine (METH). Acrylamide Hb adducts were determined, and a significant increase ( p < 0.05) in acrylamide Hb adduct levels was observed 24 h following treatment with FeSO 4 and 72 h following treatment with MPTP or METH. The results of this study show that acrylamide Hb adduct levels are increased in mice treated with compounds known to induce free radicals, thus suggesting the endogenous production of acrylamide.
Tareke
Eden
E
Division of Personalized Nutrition and Medicine, Toxicologic Pathology Association, Inc., Jefferson, Arkansas 72079, USA. eden.tareke@fda.hhs.gov
Lyn-Cook
Beverly
B
Robinson
Bonnie
B
Ali
Syed F
SF
D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine / Q000494:pharmacology; D020106:Acrylamide / Q000008:administration & dosage / Q000097:blood / Q000138:chemical synthesis*; D000818:Animals; D002273:Carcinogens / Q000138:chemical synthesis*; D004032:Diet; D005296:Ferrous Compounds / Q000494:pharmacology; D005504:Food Analysis; D005511:Food Handling / Q000379:methods*; D005914:Globins / Q000737:chemistry / Q000302:isolation & purification; D006454:Hemoglobins / Q000737:chemistry; D006358:Hot Temperature; D008297:Male; D008694:Methamphetamine / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D014633:Valine / Q000737:chemistry
D002273:Carcinogens; D005296:Ferrous Compounds; D006454:Hemoglobins; D020106:Acrylamide; C020748:ferrous sulfate; D008694:Methamphetamine; D005914:Globins; D015632:1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine; D014633:Valine