Characterization of subcellular localization and Ca2+ modulation of calsenilin/DREAM/KChIP3.
Earlier reports found that calsenilin is a transcriptional repressor or a subunit of plasma membrane channel, and indicated that calsenilin was present in the nucleus or plasma membrane. Immunohistochemical and subcellular fractionation analysis, however, revealed that calsenilin/DREAM/KChIP3 was distributed throughout the cytoplasm of SK-N-BE2(C), Jurkat, and HeLa cells. In addition, the expression of calsenilin suppressed the ATP-induced increase in intracellular Ca2+ concentrations. By increase in intracellular calcium concentration, calsenilin was translocated into the nucleus.
Woo
Ha-Na
HN
College of Pharmacy, Sungkyunkwan University, Jangan-gu, Suwon, Korea.
Chang
Jae-Woong
JW
Choi
Yun-Hyung
YH
Gwon
A-Ryeong
AR
Jung
Yong-Keun
YK
Jo
Dong-Gyu
DG
D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D042541:Intracellular Space / Q000378:metabolism; D019169:Jurkat Cells; D051605:Kv Channel-Interacting Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
C496749:KCNIP3 protein, human; D051605:Kv Channel-Interacting Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D049452:Green Fluorescent Proteins; D002118:Calcium