title: Influence of adult Schwann cells and olfactory ensheathing glia on axon--target cell interactions in the CNS: a comparative analysis using a retinotectal co-graft model.
abstract: We used an in vivo transplant approach to examine how adult Schwann cells and olfactory ensheathing glia OEG influence the specificity of axon-target cell interactions when they are introduced into the CNS. Populations of either Schwann cells or OEG were mixed with dissociated fetal tectal cells presumptive superior colliculus and, after reaggregation, pieces were grafted onto newborn rat superior colliculus. Both glial types were prelabeled with lentiviral vectors encoding green fluorescent protein. Grafts rapidly established fiber connections with the host and retinal projections into co-grafts were assessed 656 days posttransplantation by injecting cholera toxin B into host eyes. In control rats that received pure dissociated-reaggregated tectal grafts, retinal ganglion cell RGC axons selectively innervated defined target areas, corresponding to the retinorecipient layer in normal superior colliculus. The pattern of RGC axon ingrowth into OEG containing co-grafts was similar to that in control grafts. However, in Schwann cell co-grafts there was reduced host retinal input into presumptive target areas and many RGC axons were scattered throughout the neuropil. Given that OEG in co-grafts had minimal impact on axon-target cell recognition, OEG might be an appropriate cell type for direct transplantation into injured neuropil when attempting to stimulate specific pathway reconstruction.
authors.affiliation: School of Anatomy and Human Biology and Reds Spinal Cord Research Laboratory, The University of Western Australia, Crawley, Australia.
authors.forename: Jana
authors.initials: J
authors.lastname: Vukovic
authors.forename: Giles W
authors.initials: GW
authors.lastname: Plant
authors.forename: Marc J
authors.initials: MJ
authors.lastname: Ruitenberg
authors.forename: Alan R
authors.initials: AR
authors.lastname: Harvey