title: Polymorphism of prion protein gene in Arctic fox (Vulpes lagopus).
abstract: Prion diseases are fatal neurodegenerative disorders of humans and certain other mammals. Prion protein gene (Prnp) is associated with susceptibility and species barrier to prion diseases. No natural and experimental prion diseases have been documented to date in Arctic fox. In the present study, coding region of Prnp from 135 Arctic foxes were cloned and screened for polymorphisms. Our results indicated that the Arctic fox Prnp open reading frame (ORF) contains 771 nucleotides encoding 257 amino acids. Four single nucleotide polymorphisms (SNPs) (G312C, A337G, C541T, and A723G) were identified. SNPs G312C and A723G produced silent mutations, but SNPs A337G and C541T resulted in a M-V change at codon 113 and R-C at codon 181, respectively. The Arctic fox Prnp amino acid sequence was similar to that of the dog (XM 542906). In short, this study provides preliminary information about genotypes of Prnp in Arctic fox.
authors.affiliation: Institute of Military Veterinary, Academy of Military Medical Sciences, 1068 Qinglong Road, Changchun, 130062, China.
authors.forename: Jiayu
authors.initials: J
authors.lastname: Wan
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.forename: Wensen
authors.initials: W
authors.lastname: Liu
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.forename: Ming
authors.initials: M
authors.lastname: Xu
authors.forename: Hongwei
authors.initials: H
authors.lastname: Gao
mesh_terms: D019943:Amino Acid Substitution; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005589:Foxes / Q000235:genetics*; D005838:Genotype; D008969:Molecular Sequence Data; D016366:Open Reading Frames / Q000235:genetics; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D011328:Prions / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D011328:Prions