title: Consequence of the loss of Sox2 in the developing brain of the mouse.
abstract: The transcription factor Sox2 is expressed at high levels in neural stem and progenitor cells. Here, we inactivated Sox2 specifically in the developing brain by using Cre-loxP system. Although mutant animals did not survive after birth, analysis of late gestation embryos revealed that loss of Sox2 causes enlargement of the lateral ventricles and a decrease in the number of neurosphere-forming cells. However, although their neurogenic potential is attenuated, Sox2-deficient neural stem cells retain their multipotency and self-renewal capacity. We found that expression level of Sox3 is elevated in Sox2 null developing brain, probably mitigating the effects of loss of Sox2.
authors.affiliation: Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka, Saitama 350-1241, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Miyagi
authors.forename: Shinji
authors.initials: S
authors.lastname: Masui
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Niwa
authors.forename: Tetsuichiro
authors.initials: T
authors.lastname: Saito
authors.forename: Takuya
authors.initials: T
authors.lastname: Shimazaki
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Okano
authors.forename: Masazumi
authors.initials: M
authors.lastname: Nishimoto
authors.forename: Masami
authors.initials: M
authors.lastname: Muramatsu
authors.forename: Atsushi
authors.initials: A
authors.lastname: Iwama
authors.forename: Akihiko
authors.initials: A
authors.lastname: Okuda
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D020868:Gene Silencing; D024002:HMGB Proteins / Q000235:genetics / Q000502:physiology*; D006609:High Mobility Group Proteins / Q000096:biosynthesis; D020547:Lateral Ventricles / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism; D055748:SOXB1 Transcription Factors; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
chemical_list: D004268:DNA-Binding Proteins; D024002:HMGB Proteins; D006609:High Mobility Group Proteins; D051880:Receptors, Notch; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; C527786:Sox3 protein, mouse; D014157:Transcription Factors