title: Senescence-Accelerated Mouse (SAM) strains have a spontaneous mutation in the Abcb1a gene.
abstract: Senescence-Accelerated Mouse (SAM) strains are used as animal models for gerontological research. Here, we report that the SAMR1 strain, which shows a high sensitivity to toxicity of the parasiticide ivermectin, has a spontaneous retroviral insertional mutation in the ATP-binding cassette, sub-family B (MDR/TAP), member 1A (Abcb1a) gene. This mutation is identical to that found in Crl:CF1-Abcb1a mice, which are also highly sensitive to ivermectin due to the mutation. The mutant Abcb1a allele was found in SAMR4, SAMR5, SAMP1, SAMP6, SAMP7, and SAMP9, but not in SAMP3, SAMP8, SAMP10, SAMP11, and other outbred and inbred strains, including 129/SvJ strains. These results impart both caution and promise in the use of SAM strains in studies of biological processes in which P-glycoprotein participates.
authors.affiliation: Department of Aging Biology, Institute on Aging and Adaptation, Shinshu University Graduate School of Medicine, Matsumoto, Japan.
authors.forename: Guohong
authors.initials: G
authors.lastname: Zhang
authors.forename: Beiru
authors.initials: B
authors.lastname: Zhang
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Fu
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tomozawa
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Keiichi
authors.initials: K
authors.lastname: Higuchi
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mori
mesh_terms: D019588:Aging, Premature / Q000235:genetics / Q000662:veterinary*; D000483:Alleles; D000818:Animals; D000977:Antiparasitic Agents / Q000494:pharmacology; D004195:Disease Models, Animal; D007559:Ivermectin / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains / Q000235:genetics*; D009154:Mutation; D012376:Rodent Diseases / Q000235:genetics*
chemical_list: D000977:Antiparasitic Agents; D007559:Ivermectin