Concentration of bone marrow aspirate for osteogenic repair using simple centrifugal methods.
Bone marrow transplantation can lead to osteogenic repair of intractable bone conditions. To achieve optimal clinical results, it is necessary to transplant as many cells with osteogenetic potential as possible. However, approaches involving special equipment and reagents for the extraction and purification of cells are expensive, and the complicated procedures involved are a hindrance to widespread acceptance of bone marrow transplantation for osteogenic repair. To standardize bone marrow transplantation for bone regeneration, a simple, safe, clean, and low-cost system is required. We describe an easy-to-use method using a conventional manual blood bag centrifugation technique traditionally used for extracting buffy coats, for concentration of cells from bone marrow aspirates (BMAs) to obtain osteogenic progenitors.
Sakai
Shinisuke
S
Department of Orthopedic Surgery, Control of Musculoskeletal System, Advanced Biomedical Applications, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan.
Mishima
Hajime
H
Ishii
Tomoo
T
Akaogi
Hiroshi
H
Yoshioka
Tomokazu
T
Uemura
Toshimasa
T
Ochiai
Naoyuki
N
D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation / Q000379:methods*; D002469:Cell Separation / Q000379:methods; D002498:Centrifugation / Q000379:methods*; D006801:Humans; D010012:Osteogenesis / Q000502:physiology; D013234:Stem Cells / Q000166:cytology*; D020858:Tissue and Organ Harvesting / Q000379:methods