Brain endocannabinoid system is involved in fluoxetine-induced anorexia.
In order to describe the effects of chronic fluoxetine administration on the brain endocannabinoid system in lean and obese Zucker rats, brain immunostaining for the CB1 and CB1-phosphorylated cannabinoid receptors was carried out. Obese Zucker rats showed significantly increased the numbers of neural cells positively immunostained for the CB1-phosphorylated receptor in the striatum, compared to their lean litter-mates. Chronic fluoxetine administration decreased the number of neural cells immunostained for CB1-phosphorylated receptor in several striatal and hippocampal regions of obese Zucker rats, compared to controls treated with saline. In contrast, no change in CB1-phosphorylated receptor immunostaining was observed in fluoxetine-treated lean rats, with respect to controls. Taken together, these results suggest the involvement of the hippocampal and striatal endocannabinoid receptor system in fluoxetine-induced anorexia in lean and obese Zucker rats.
Zarate
Jon
J
Department of Physiology, Faculty of Pharmacy, University of the Basque Country, Vitoria-Gasteiz, Spain. jon.zarate@ehu.es
Churruca
Itziar
I
Pascual
Jesús
J
Casis
Luis
L
Sallés
Joan
J
Echevarría
Enrique
E
D000818:Animals; D000855:Anorexia / Q000139:chemically induced* / Q000503:physiopathology; D001921:Brain / Q000503:physiopathology*; D001923:Brain Chemistry; D063385:Cannabinoid Receptor Modulators / Q000502:physiology*; D003342:Corpus Striatum / Q000737:chemistry; D063388:Endocannabinoids; D005473:Fluoxetine / Q000008:administration & dosage*; D006624:Hippocampus / Q000737:chemistry; D008297:Male; D009765:Obesity / Q000503:physiopathology; D010766:Phosphorylation; D051381:Rats; D011924:Rats, Zucker; D043884:Receptor, Cannabinoid, CB1 / Q000032:analysis / Q000737:chemistry / Q000187:drug effects
D063385:Cannabinoid Receptor Modulators; D063388:Endocannabinoids; D043884:Receptor, Cannabinoid, CB1; D005473:Fluoxetine