Ligand binding mode of GABAA receptor-associated protein.
The gamma-aminobutyric acid type A (GABA(A)) receptor-associated protein is a versatile adaptor protein playing an important role in intracellular vesicle trafficking, particularly in neuronal cells. We present the X-ray structure of the soluble form of human GABA(A) receptor-associated protein complexed with a high-affinity synthetic peptide at 1.3 A resolution. The data shed light on the probable binding modes of key interaction partners, including the GABA(A) receptor and the cysteine protease Atg4. The resulting models provide a structural background for further investigation of the unique biological properties of this protein.
Weiergräber
Oliver H
OH
Institut für Neurowissenschaften und Biophysik, Molekulare Biophysik, Forschungszentrum Jülich, D-52425 Jülich, Germany.
Stangler
Thomas
T
Thielmann
Yvonne
Y
Mohrlüder
Jeannine
J
Wiesehan
Katja
K
Willbold
Dieter
D
D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins; D006801:Humans; D007700:Kinetics; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D020349:Surface Plasmon Resonance
D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C116741:GABARAP protein, human; D008024:Ligands; D008869:Microtubule-Associated Proteins; D010455:Peptides