The genome-linked protein of cowpea mosaic virus is coded by RNA from the bottom component.
It has recently been shown that RNA from the bottom component of cowpea mosaic virus is capable of self-replication in cowpea mesophyll protoplasts, providing a tool for the investigation of the contribution of each RNA strand toward viral multiplication. Evidence is presented here, using this system, to show that the genome-linked protein is coded by the RNA from the bottom component. As the replicase function is coded by this RNA, the results remain consistent with the involvement of the genome-linked protein in viral RNA replication.
Stanley
J
J
Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands.
Goldbach
R
R
Van Kammen
A
A