title: 5-Fluorouracil-related gene expression in primary sites and hepatic metastases of colorectal carcinomas.
abstract: The aim of this study was to investigate the correlation of the mRNA expressions of 5-fluorouracil (5FU)-related genes in the primary sites and liver metastases of colorectal carcinomas.
  
  
  METHODS
  Patients with liver metastases from colorectal carcinomas were included (n = 43). The expression ratios to beta-actin of mRNA of thymidine synthase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP) and oroteta phosphoribosyl transferase (OPRT) were measured in primary and liver metastases of colorectal carcinomas by laser-captured microdissection and real time PCR.
  
  
  RESULTS
  The ratios for the expression of TS, DPD, TP and OPRT mRNAs were significantly correlated between paired primary sites and liver metastases. The mRNA expression ratios of DPD and TP showed a significant correlation both in primary sites and in liver metastases.
  
  
  CONCLUSIONS
  Enzymes of the primary colorectal carcinomas can be used in predicting the therapeutic efficacy of 5FU against liver metastases.
authors.affiliation: Department of Surgery, Gunma Cancer Center, 617-1 Takabayashi-nishi, Ota, Gunma 373-8550, Japan. shinsame@gunma-cc.jp
authors.forename: Shinichi
authors.initials: S
authors.lastname: Sameshima
authors.forename: Shigeru
authors.initials: S
authors.lastname: Tomozawa
authors.forename: Masaru
authors.initials: M
authors.lastname: Kojima
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Koketsu
authors.forename: Kenta
authors.initials: K
authors.lastname: Motegi
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Horikoshi
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Okada
authors.forename: Youichi
authors.initials: Y
authors.lastname: Kon
authors.forename: Toshio
authors.initials: T
authors.lastname: Sawada
mesh_terms: D000199:Actins / Q000096:biosynthesis / Q000235:genetics; D000964:Antimetabolites, Antineoplastic / Q000493:pharmacokinetics / Q000494:pharmacology*; D015179:Colorectal Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D042943:Dihydrouracil Dehydrogenase (NADP) / Q000096:biosynthesis / Q000235:genetics; D005260:Female; D005472:Fluorouracil / Q000493:pharmacokinetics / Q000494:pharmacology*; D015870:Gene Expression; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000201:enzymology* / Q000235:genetics / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D009962:Orotate Phosphoribosyltransferase / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013939:Thymidine Phosphorylase / Q000096:biosynthesis / Q000235:genetics; D013940:Thymidylate Synthase / Q000096:biosynthesis / Q000235:genetics
chemical_list: D000199:Actins; D000964:Antimetabolites, Antineoplastic; D012333:RNA, Messenger; D042943:Dihydrouracil Dehydrogenase (NADP); D013940:Thymidylate Synthase; D009962:Orotate Phosphoribosyltransferase; D013939:Thymidine Phosphorylase; D005472:Fluorouracil