[Determination of 16S rRNA gene sequence for a new ANAMMOX bacterial species].
The anaerobic ammonium oxidation (ANAMMOX) activity of the sludge was about 9.84 x 10(-4) mg x (mg x h)(-1) by measuring the simultaneous consumption of ammonium and nitrite under anoxic conditions in the batch tests. The consumption of NO2(-) -N and NH4+ -N was 1.311 for ANAMMOX bacteria. The partial 16S rDNA sequence was obtained by using molecule biology methods. Crude DNA of the total bacteria in granular sludge from EGSB reactor was extracted and purified. Then, PCR amplification by using specific primer, clone and sequence determination was performed. ANAMMOX bacterial species(anaerobic ammonium-oxidizing Planctomycete cquenviron-1) which was enrichment cultivated from EGSB reactor were the same genera with Candidatus "Anammoxoglobus propionicus" and Candidatus "Jettenia asiatica" by analyzing phylogenetic tree. The maximum identities of anaerobic ammonium-oxidizing Planctomycete cquenviron-1 with other ANAMMOX bacterial species was about 93%. The results showed that a new ANAMMOX bacterial species which was enrichment cultivated from EGSB reactor was found and anaerobic ammonium-oxidizing Planctomycete cquenviron-1 was denominated.
Zu
Bo
B
Department of Environmental Science, Chongqing University, Chongqing 400030, China. boby123321@sina.com.cn
Zhang
Dai-jun
DJ
Yan
Qing
Q
D000693:Anaerobiosis; D001421:Bacteria, Anaerobic / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology; D009584:Nitrogen / Q000378:metabolism*; D010802:Phylogeny; D000644:Quaternary Ammonium Compounds / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology; D014865:Waste Disposal, Fluid / Q000379:methods
D000644:Quaternary Ammonium Compounds; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D009584:Nitrogen