Protein separation using preparative-scale dynamic field gradient focusing.
Dynamic field gradient focusing uses an electric field gradient to separate and concentrate proteins in native buffers. A prototype preparative-scale dynamic field gradient focusing apparatus reproducibly separated hemoglobin and bovine serum albumin with a mean resolution of 2.64+/-0.503. Run-to-run variations in the hemoglobin's focal point and peak width appeared to be related to fluctuations in the shape of the electric field, rather than the 5% accuracy of the pump that provided the counter-flow in the separation annulus. The variation in the electric field gradient was probably due to the formation and expansion of an ion-depleted region at the top of the separation annulus.
Tracy
Noah I
NI
School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA 99164-2710, USA.
Ivory
Cornelius F
CF
D004586:Electrophoresis / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000302:isolation & purification; D006454:Hemoglobins / Q000302:isolation & purification; D007525:Isoelectric Focusing / Q000379:methods*; D016014:Linear Models; D011506:Proteins / Q000302:isolation & purification*; D012710:Serum Albumin, Bovine / Q000302:isolation & purification
D006454:Hemoglobins; D011506:Proteins; C039217:fluorescein isothiocyanate bovine serum albumin; D012710:Serum Albumin, Bovine; D016650:Fluorescein-5-isothiocyanate