Rapid and sensitive quantitation of morphine using HPLC with electrochemical detection.
BACKGROUND
Morphine is one of the most widely used opioid analgesics for controlling pain in cancer and post-operative patients.


OBJECTIVE
This study aimed at finding a sensitive method for measuring morphine.


METHODS
A one-step solid phase extraction was developed for extracting morphine from various samples, and morphine concentration was measured using a high-performance liquid chromatographic system with electrochemical detection. The sensitivity of the assay was 1.53 ng/mL with a recovery of 93.4% +/- 0.01. The mean intra-assay and inter-assay test for three concentrations was 10.54 and 7.47, respectively. The assay showed no cross-reaction with a wide range of compounds.


CONCLUSIONS
This method for morphine in small biological samples is easy, sensitive and reproducible with low cross-reactivity.
Mashayekhi
S O
SO
Faculty of Pharmacy and Drug Applied Research Center, Hematology & Oncology Research Centre, NPMC, Tabriz University of Medical Sciences, Tabriz, Iran. mashayekhis@yahoo.co.uk
Ghandforoush-Sattari
M
M
Hain
R D W
RD
D000701:Analgesics, Opioid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D006801:Humans; D009020:Morphine / Q000032:analysis*; D015203:Reproducibility of Results
D000701:Analgesics, Opioid; D009020:Morphine