Microarray probe expression measures, data normalization and statistical validation.
DNA microarray technology is a high-throughput method for gaining information on gene function. Microarray technology is based on deposition/synthesis, in an ordered manner, on a solid surface, of thousands of EST sequences/genes/oligonucleotides. Due to the high number of generated datapoints, computational tools are essential in microarray data analysis and mining to grasp knowledge from experimental results. In this review, we will focus on some of the methodologies actually available to define gene expression intensity measures, microarray data normalization, and statistical validation of differential expression.
Saviozzi
Silvia
S
Department of Biological and Clinical Sciences, University of Torino, c/o Az. Ospedaliera S. Luigi Regione Gonzole 10, Orbassano (TO) 10043, Italy.
Calogero
Raffaele A
RA