Hepatic metabolism of glucose and linoleic acid varies in relation to susceptibility to fatty liver in ad libitum-fed Muscovy and Pekin ducks.
The susceptibility to develop hepatic steatosis is known to differ between duck species, especially between Muscovy and Pekin ducks. This difference could be explained by either differential responses of species to overfeeding or genetic differences in hepatic lipid metabolism. The aim of the present study was to compare the intensities of the different hepatic pathways (oxidation, lipogenesis, esterification, secretion, etc.) of the two main nutrients (glucose and linoleic acid (LA)) reaching the liver of ad libitum-fed Muscovy (n 6) and Pekin (n 6) ducks using the ex vivo method of liver slices incubated for 16 h with [U-14C]glucose, [1-14C]LA and [35S]methionine added to the survival medium. In such experimental conditions, the lipogenesis pathway from glucose was 2-fold higher (P<0.05) in the liver of the Muscovy duck than in that of the Pekin duck. Furthermore, the hepatic uptake of LA was 2-fold higher (P<0.05) in the Muscovy duck than in the Pekin duck leading to a 2-fold higher (P<0.05) esterification of this fatty acid in the liver of the Muscovy duck. The hepatic secretion of VLDL was higher (P<0.01) in the Muscovy duck than in the Pekin duck but insufficient to prevent lipid accumulation in the liver of the Muscovy duck. In conclusion, these results show the influence of the species on the hepatic metabolism of ducks in relation to their susceptibility to develop fatty liver. These results should shed light on the metabolic regulations that might underlie susceptibility to hepatic steatosis in the the human liver.
Saez
Gladys
G
IPREM-EEM, IUT des Pays de l'Adour, 371 rue du ruisseau, BP 201, 40004 Mont de Marsan Cedex, France.
Baéza
Elisabeth
E
Davail
Stéphane
S
Durand
Denys
D
Bauchart
Dominique
D
Gruffat
Dominique
D
D000821:Animal Feed; D000818:Animals; D004198:Disease Susceptibility; D004372:Ducks; D005234:Fatty Liver / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D005838:Genotype; D005947:Glucose / Q000378:metabolism* / Q000494:pharmacology; D019787:Linoleic Acid / Q000378:metabolism* / Q000494:pharmacology; D008079:Lipoproteins, VLDL / Q000378:metabolism; D008099:Liver / Q000378:metabolism*; D008715:Methionine / Q000494:pharmacology; D023421:Models, Animal; D011201:Poultry Diseases / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D046509:Tissue Culture Techniques
D008079:Lipoproteins, VLDL; D019787:Linoleic Acid; D008715:Methionine; D005947:Glucose