title: The polypeptides induced in Drosophila cells by Drosophila C virus (strain Ouarzazate).
abstract: The Ouarzazate strain of Drosophila virus (DCV0) was grown in Drosophila melanogaster tissue culture cells, and [35S]methionine-labeled virions were found to contain a group of major structural proteins with a molecular weight of approximately 30,000 as well as several minor proteins of higher molecular weight and a protein of approximately 10,000 daltons. Using a range of pulses, chases and gel systems, examination of the intracellular proteins induced by DCV0 showed the presence of 17 polypeptides not found in uninfected cells. The synthesis of virus-induced polypeptides was extremely asymmetric with a rapid appearance of the major virus structural proteins and a much slower appearance of the lowest molecular weight structural protein (VP4). Processing of virus-induced proteins including the appearance of VP4 was demonstrated using pulse-chase after pulsing with [35S]methionine. While the highest molecular weight induced protein found in infected cells was 146,000, pretreatment of cells with iodoacetamide resulted in the appearance of a protein with a molecular weight of approximately 200,000. The evidence presented in this paper supports the inclusion of DCV0 in the Picornaviridae group.
authors.affiliation: Natural Environment Research Council, Institute of Virology, 5 South Parks Road, Oxford OX1 3UB, United Kingdom.
authors.forename: N F
authors.initials: NF
authors.lastname: Moore
authors.forename: B
authors.initials: B
authors.lastname: Reavy
authors.forename: J S
authors.initials: JS
authors.lastname: Pullin
authors.forename: N
authors.initials: N
authors.lastname: Plus