title: Negative feedback regulation of colitogenic CD4+ T cells by increased granulopoiesis.
abstract: BACKGROUND
  Chronic inflammatory diseases are characterized by massive infiltration of innate and acquired immune cells in inflammatory sites. However, it remains unclear how these cells cooperate in the development of disease. Although bone marrow (BM) is a primary site for hematopoiesis of immune cells except T cells, BM recruits memory T cells from the periphery. We have recently demonstrated that colitogenic CD4(+) memory T cells reside in BM of colitic CD4(+)CD45RB(high) T-cell-transferred SCID mice. Based on this background we here investigate whether granulocytes promote or suppress the expansion of colitogenic CD4(+) T cells.
  
  
  METHODS
  First, we show that Gr-1(high)CD11b(+) granulocytes were significantly increased in colitic BM along with a significant increase of peripheral granulocytes. Consistently, the colony-forming unit (CFU) assay revealed that granulocyte colony formation was dominantly induced by supernatants from anti-CD3-stimulated colitic BM CD4(+) T cells.
  
  
  RESULTS
  Administration of granulocyte-depleting anti-Gr-1 mAb to colitic mice did not ameliorate the colitis, but exacerbated the wasting disease with an increased expansion of systemic, but not lamina propria, CD4(+) T cells with activated phenotype.
  
  
  CONCLUSIONS
  These results suggest that the increased granulopoiesis by colitogenic BM CD4(+) T cells represent a negative feedback mechanism to control systemic inflammation.
authors.affiliation: Department of Gastroenterology and Hepatology, Graduate School of Medicine, Tokyo Medical and Dental University, Juntendo University School of Medicine, Tokyo, Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Nemoto
authors.forename: Takanori
authors.initials: T
authors.lastname: Kanai
authors.forename: Shuji
authors.initials: S
authors.lastname: Tohda
authors.forename: Teruji
authors.initials: T
authors.lastname: Totsuka
authors.forename: Ryuichi
authors.initials: R
authors.lastname: Okamoto
authors.forename: Kiichiro
authors.initials: K
authors.lastname: Tsuchiya
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakamura
authors.forename: Naoya
authors.initials: N
authors.lastname: Sakamoto
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Fukuda
authors.forename: Osamu
authors.initials: O
authors.lastname: Miura
authors.forename: Hideo
authors.initials: H
authors.lastname: Yagita
authors.forename: Mamoru
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology* / Q000502:physiology; D002478:Cells, Cultured; D002908:Chronic Disease; D003092:Colitis / Q000276:immunology* / Q000503:physiopathology; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D025461:Feedback, Physiological / Q000276:immunology* / Q000502:physiology; D005434:Flow Cytometry; D006098:Granulocytes / Q000276:immunology* / Q000502:physiology; D007150:Immunohistochemistry; D019891:Leukopoiesis / Q000276:immunology* / Q000502:physiology; D008213:Lymphocyte Activation; D051379:Mice; D008807:Mice, Inbred BALB C; D016513:Mice, SCID; D011336:Probability; D011897:Random Allocation; D012680:Sensitivity and Specificity; D013156:Splenectomy; D018709:Statistics, Nonparametric