Chitosan microparticles as injectable scaffolds for tissue engineering.
The use of chitosan microparticles as injectable carriers for cell transplantation represents a promising alternative to avoid the drawbacks of the implantation of other forms of three-dimensional (3D) scaffolds seeded with cells. In this study, a 3D construct is obtained in vitro by combining chitosan microparticles crosslinked with genipin and goat bone marrow stromal cells (GBMCs). Cell viability and the morphology of GBMCs were evaluated after culture for 7 and 14 days. Our results show the feasibility of chitosan microparticles as potential injectable scaffolds for tissue engineering and regenerative medicine.
Cruz
Dunia Mercedes García
DM
Centre for Biomaterials, Universidad Politécnica de Valencia, 46022 Valencia, Spain.
Ivirico
Jorge Luis Escobar
JL
Gomes
Manuela M
MM
Ribelles
Jose Luis Gómez
JL
Sánchez
Manuel Salmerón
MS
Reis
Rui L
RL
Mano
João F
JF
D000818:Animals; D001854:Bone Marrow Cells; D002478:Cells, Cultured; D048271:Chitosan / Q000008:administration & dosage*; D006041:Goats; D007267:Injections; D008855:Microscopy, Electron, Scanning; D010316:Particle Size; D017154:Stromal Cells; D023822:Tissue Engineering / Q000295:instrumentation* / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D048271:Chitosan