title: [Simple and rapid microbiological method for determination of residual antibacterials in meat].
abstract: A simple and rapid screening method using bioassay for the simultaneous analysis of antibacterials (penicillins, cephalosporins, macrolides, tetracyclines, quinolones, etc.) in meat has been developed. A 5 g sample was homogenized with 5 mL of methanol, and the homogenate was centrifuged for 10 min with 3,000 rpm. The pulp disk method with Bacillus subtilis BGA (Antibiotic Medium 5 (pH 8) and 8 (pH 6)), Micrococcus luteus ATCC 9341 and Geobacillus stearothermophilus as test organisms was employed for the assay of the antibacterials. Typical antibacterials (penicillin G, ampicillin, cefapirin, cefalexin, erythromycin, spiramycin, oxytetracycline, chlortetracycline, streptomycin, dihydrostreptomycin, enrofloxacin and oxolinic acid) were detected at levels of ca. 0.005-2.5 microg/g in meat. Therefore, we recommend this proposed screening method for routine analysis of residual antibacterials in livestock products.
authors.affiliation: Saitama Prefectural Institute of Public Health, Saitama, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Horie
authors.forename: Harumi
authors.initials: H
authors.lastname: Kobayashi
authors.forename: Rie
authors.initials: R
authors.lastname: Ishii
authors.forename: Akihiro
authors.initials: A
authors.lastname: Ibe
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Fujita
authors.forename: Kenji
authors.initials: K
authors.lastname: Tanno
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakazawa
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis*; D001412:Bacillus subtilis / Q000187:drug effects; D001431:Bacteriological Techniques / Q000379:methods*; D001681:Biological Assay / Q000379:methods*; D008460:Meat / Q000032:analysis*; D016982:Micrococcus luteus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents