The role of microtubule movement in bidirectional organelle transport.
We study the role of microtubule movement in bidirectional organelle transport in Drosophila S2 cells and show that EGFP-tagged peroxisomes in cells serve as sensitive probes of motor induced, noisy cytoskeletal motions. Multiple peroxisomes move in unison over large time windows and show correlations with microtubule tip positions, indicating rapid microtubule fluctuations in the longitudinal direction. We report the first high-resolution measurement of longitudinal microtubule fluctuations performed by tracing such pairs of co-moving peroxisomes. The resulting picture shows that motor-dependent longitudinal microtubule oscillations contribute significantly to cargo movement along microtubules. Thus, contrary to the conventional view, organelle transport cannot be described solely in terms of cargo movement along stationary microtubule tracks, but instead includes a strong contribution from the movement of the tracks.
Kulic
Igor M
IM
School of Engineering and Applied Sciences, 29 Oxford Street, Pierce Hall 409, Harvard University, Cambridge, MA 02138, USA. kulic@seas.harvard.edu
Brown
André E X
AE
Kim
Hwajin
H
Kural
Comert
C
Blehm
Benjamin
B
Selvin
Paul R
PR
Nelson
Philip C
PC
Gelfand
Vladimir I
VI
D000818:Animals; D001693:Biological Transport, Active; D055592:Biophysical Phenomena; D001703:Biophysics; D002460:Cell Line; D003599:Cytoskeleton / Q000502:physiology; D004330:Drosophila; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008856:Microscopy, Fluorescence; D018715:Microscopy, Video; D008870:Microtubules / Q000502:physiology*; D008954:Models, Biological; D020409:Molecular Motor Proteins / Q000502:physiology; D009068:Movement; D015388:Organelles / Q000502:physiology*; D020675:Peroxisomes / Q000502:physiology; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
D020409:Molecular Motor Proteins; D011994:Recombinant Proteins; C485184:enhanced green fluorescent protein; D049452:Green Fluorescent Proteins