Reassembly of particles using the RNA from partially disassembled tobacco mosaic virus.
Controlled alkaline disassembly of tobacco mosaic virus (TMV) results in the production of two populations of partially stripped virus. These particles have been designated PSV5 and 6 and are the two smallest particles produced during alkaline disassembly of TMV. The RNA from these particles was able to reassemble with TMV coat protein. The reassembled particles were resistant to ribonuclease treatment and sedimented upon sucrose density gradient centrifugation to the same position as authentic PSV5 and 6 particles.
Pelcher
L E
LE
Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Saskatchewan, S7N 0W9, Canada.
Halasa
M C
MC