title: Gene expression of nestin, collagen type I and type III in human dental follicle cells after cultivation in serum-free medium.
abstract: BACKGROUND
  Undifferentiated human dental cells and especially human dental follicle cells are interesting for potential dental treatments. These somatic stem cells are cultured usually in cell culture medium containing bovine serum. In the age of bovine spongiform encephalopathy (BSE), a serum-free cell culture system for dental follicle cells are recommended, if these cells will be applied in dentistry.
  
  
  OBJECTIVE
  However, less is known about the cultivation of dental follicle cells in serum-replacement medium. In this study, we cultivated dental follicle cells in serum-free cell culture medium, which is normally applied for neuronal stem/progenitor cells.
  
  
  METHODS
  Dental follicle cells were cultivated in both serum-free and serum-containing cell culture media, and gene expression profiles were recorded for connective tissue markers collagen type I and type III and for the human dental follicle cell marker nestin.
  
  
  RESULTS
  It is interesting to note that the gene expressions of collagens and nestin were similar after applying both cell culture conditions.
  
  
  CONCLUSIONS
  Although the gene expression of dental follicle cell markers was unchanged, a more appropriate serum-free cell culture medium is recommended for cell proliferation of dental follicle cells.
authors.affiliation: Institut für Humangenetik, Universität Regensburg, Franz-Josef-Strauss-Allee 11, Regensburg, Germany. Christian.morsczeck@klinik.uni-regensburg.de
authors.forename: Christian
authors.initials: C
authors.lastname: Morsczeck
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Ernst
authors.forename: Christian
authors.initials: C
authors.lastname: Florian
authors.forename: Torsten Eugen
authors.initials: TE
authors.lastname: Reichert
authors.forename: Peter
authors.initials: P
authors.lastname: Proff
authors.forename: Richard
authors.initials: R
authors.lastname: Bauer
authors.forename: Urs
authors.initials: U
authors.lastname: Müller-Richter
authors.forename: Oliver
authors.initials: O
authors.lastname: Driemel
mesh_terms: D015415:Biomarkers / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism*; D024061:Collagen Type III / Q000235:genetics / Q000378:metabolism*; D003795:Dental Sac / Q000166:cytology / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000502:physiology; D006801:Humans; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism*; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D064231:Nestin; D012313:RNA / Q000032:analysis; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D024042:Collagen Type I; D024061:Collagen Type III; D007381:Intermediate Filament Proteins; C577484:NES protein, human; D009419:Nerve Tissue Proteins; D064231:Nestin; D012313:RNA