Annexin A2 regulates the levels of plasmin, S100A10 and Fascin in L5178Y cells.
Annexin A2 (ANXA2) was reported as the receptor, activator, expression enhancer, or cooperator for plasmin, S100A10, and others. To delineate the effect of ANXA2 on the proteins that are probably associated with tumor development and metastasis by a credible experimental method, we generated an ANXA2 gene knockout tumor cell line, ANXA2(-/-) L5178Y, and compared the expression levels of plasmin, S100A10 and fascin in the generated cell line with in wild type of L5178Y at mRNA and protein levels. The results showed that the mRNA level of plasminogen (PLG) was not substantially changed in cultured ANXA2(-/-) cells, but the protein level of plasmin was significantly lower in the cultured ANXA2(-/-) cells than in cultured ANXA2(+/+) cells. For S100A10 and fascin, their mRNA and protein levels were significantly lower in the cultured ANXA2(-/-) cells than in cultured ANXA2(+/+) cells. Results indicate that ANXA2 introduces the generation or expression of plasmin, S100A10, and fascin in tumor cells. ANXA2 affects PLG/plasmin level by a way post transcription and may be an inducer or enhancer to fascin expression at transcription level. By the regulations, ANXA2 enhances the development, invasion, and metastasis of tumor. The detailed mechanism for the regulations above remains to be further investigated, but our results show the potential of ANXA2 as a new target molecule for the strategies of tumor biotherapy or tumor gene therapy.
Hou
Yingchun
Y
Department of Cell Biology, School of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi, China. ychhou@snnu.edu.cn
Yang
Lijuan
L
Mou
Maosen
M
Hou
Yang
Y
Zhang
Airan
A
Pan
Ning
N
Qiang
Rongbin
R
Wei
Lili
L
Zhang
Nannan
N
D000818:Animals; D017306:Annexin A2 / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D016503:Drug Delivery Systems; D005341:Fibrinolysin / Q000096:biosynthesis* / Q000235:genetics; D015973:Gene Expression Regulation, Leukemic; D018390:Gene Targeting; D007940:Leukemia L5178 / Q000378:metabolism* / Q000473:pathology; D051379:Mice; D008840:Microfilament Proteins / Q000096:biosynthesis* / Q000235:genetics; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D012334:RNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000502:physiology; D009418:S100 Proteins / Q000096:biosynthesis* / Q000235:genetics; D000099248:S100 Calcium Binding Protein A10
D017306:Annexin A2; D002352:Carrier Proteins; D005341:Fibrinolysin; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; D011993:Recombinant Fusion Proteins; D009418:S100 Proteins; D000099248:S100 Calcium Binding Protein A10; C020324:fascin