[Effect of Rehmannia glutinosa Libosch water extraction on gene expression of proinsulin in type 2 diabetes mellitus rats].
OBJECTIVE
To investigate the mechanisms of treating 2-DM by Rehmannia glutinosa Libosch water extraction (RGLE).


METHODS
The mRNA level of proinsulin in rats panreas tissue was examined by semi-quatitativa RT-PCR,and the protein was measured by SDS-PAGE.


RESULTS
The mRNA and protein expressions of proinsulin in RGLE group were higher than those of diabetic model group (P<0.01). The levels of FPG decreased. FINS,IS, HbetaCI increased (P<0.01).


CONCLUSIONS
It may be the mechanism how the RGLE to decline high FPG and cure the 2-DM.
Meng
Qing-Yu
QY
Mudanjiang Medical College, Department of Parmacology, Mudanjiang 157011, China.
Lv
Xiu-Fang
XF
Jin
Xiu-Dong
XD
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy* / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use; D007328:Insulin / Q000096:biosynthesis; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism; D008297:Male; D010179:Pancreas / Q000187:drug effects / Q000378:metabolism; D010946:Plants, Medicinal / Q000737:chemistry; D011384:Proinsulin / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D032267:Rehmannia / Q000737:chemistry*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D007004:Hypoglycemic Agents; D007328:Insulin; D012333:RNA, Messenger; D011384:Proinsulin