title: Candida ratchasimensis sp. nov. and Candida khaoyaiensis sp. nov., two anamorphic yeast species isolated from flowers in Thailand.
abstract: Two yeast strains of the genus Candida were isolated from wild flowers collected in Khao Yai National Park, Nakhonratchasima Province, Thailand. Based on morphological, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 domain of the 26S rRNA gene, strains BCC 7722(T) (=NBRC 102563(T)=CBS 10611(T)) and BCC 7729(T) (=NBRC 102565(T)=CBS 10839(T)) were found to represent two distinct novel Candida species, for which the names Candida ratchasimensis sp. nov. and Candida khaoyaiensis sp. nov. are proposed, respectively. In the phylogenetic tree constructed according to the neighbour-joining method based on sequences of the D1/D2 domain of the 26S rRNA gene, strains BCC 7722(T) (GenBank accession no. AY228492) and BCC 7729(T) (accession no. DQ400367) constituted a cluster with Candida cellae that was connected with a clade with Starmerella meliponinorum and Candida etschellsii. Within the D1/D2 domain, C. ratchasimensis and C. khaoyaiensis differ from C. cellae in 25 nucleotide substitutions with five gaps and 29 nucleotide substitutions with one gap, respectively.
authors.affiliation: Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand. sasitorn@biotec.or.th
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Jindamorakot
authors.forename: Savitree
authors.initials: S
authors.lastname: Limtong
authors.forename: Wichien
authors.initials: W
authors.lastname: Yongmanitchai
authors.forename: Manee
authors.initials: M
authors.lastname: Tuntirungkij
authors.forename: Wanchern
authors.initials: W
authors.lastname: Potacharoen
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kawasaki
authors.forename: Morakot
authors.initials: M
authors.lastname: Tanticharoen
authors.forename: Takashi
authors.initials: T
authors.lastname: Nakase
mesh_terms: D002175:Candida / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D004271:DNA, Fungal / Q000032:analysis; D035264:Flowers / Q000382:microbiology*; D020459:Genes, rRNA; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D012335:RNA, Ribosomal / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D013785:Thailand
chemical_list: D004271:DNA, Fungal; D012335:RNA, Ribosomal; C065096:RNA, ribosomal, 26S