The effect of in vitro exposure to tributyltin on the immune competence of Chinook salmon (Oncorhynchus tshawytscha) leukocytes.
We evaluated the direct effects of in vitro exposures to tributyltin (TBT), a widely used biocide, on the cell-mediated immune system of Chinook salmon (Oncorhynchus tshawytscha). Splenic and pronephric leukocytes isolated from juvenile Chinook salmon were exposed to TBT (0, 0.1, 0.2, 0.3, 0.4, 0.5, and 0.6 mg/l) in cell cultures for 24 h. Effects of TBT on cell viability, induction of apoptosis, and mitogenic responses were measured by flow cytometry. Splenic and pronephric leukocytes in the presence of TBT experienced a concentration-dependent decrease in viability in cell cultures. Apoptosis was detected as one of the mechanisms of cell death after TBT exposure. In addition, pronephric lymphocytes exhibited a greater sensitivity to TBT exposure than pronephric granulocytes. The functional ability of splenic B-cells to undergo blastogenesis upon lipopolysaccharide stimulation was also significantly inhibited in the presence of 0.05, 0.07, or 0.10 mg/l of TBT in the cell cultures. Flow cytometric assay using a fluorescent conjugated monoclonal antibody against salmon surface immunoglobulin was employed for the conclusive identification of B-cells in the Chinook salmon leukocytes. Our findings suggest that adverse effects of TBT on the function or development of fish immune systems could lead to an increase in disease susceptibility and its subsequent ecological implications.
Misumi
Ichiro
I
Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA. imisumi@hawaii.edu
Yada
Takashi
T
Leong
Jo-Ann C
JA
Schreck
Carl B
CB
D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000276:immunology; D002470:Cell Survival / Q000187:drug effects / Q000276:immunology; D004305:Dose-Response Relationship, Drug; D007111:Immunity, Cellular / Q000187:drug effects; D066298:In Vitro Techniques; D007962:Leukocytes / Q000187:drug effects / Q000276:immunology*; D012474:Salmon / Q000276:immunology*; D013154:Spleen / Q000166:cytology; D014220:Trialkyltin Compounds / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D014220:Trialkyltin Compounds; D014874:Water Pollutants, Chemical; C011559:tributyltin