title: The C50T polymorphism of the cyclooxygenase-1 gene and the risk of thrombotic events during low-dose therapy with acetyl salicylic acid.
abstract: Aspirin prevents thrombotic events by inhibiting platelet cyclooxygenase-1 (COX-1), thus reducing thromboxane A2 formation and platelet aggregation. The C50T polymorphism of COX-1 is associated with an impaired inhibition of both thromboxane production and in-vitro platelet aggregation by aspirin. We studied whether this polymorphism is also associated with the risk of clinical thrombotic events in patients using aspirin. We included 496 patients admitted to our Coronary Care Unit for various indications treated with aspirin 80 mg daily. Genotyping for the C50T polymorphism demonstrated that 86.7% of the patients had the common genotype, and 13.3% had the variant (12.5% heterozygous, 0.8% homozygous). Baseline variables were well balanced, except that patients with the common genotype more frequently used aspirin prior to admission compared to those patients with the variant genotype. The composite primary endpoint of myocardial infarction, stroke, and/or cardiovascular death occurred in 98 patients (19.8%). Myocardial infarction occurred in 9.6% of patients, stroke in 1.6%, and cardiovascular death in 12.1%. The unadjusted hazard ratio (95% CI) for the primary endpoint for patients with the variant versus the common genotype was 1.07 (0.62-1.85), p = 0.8. The adjusted hazard ratio was 0.86 (0.49-1.50), p = 0.6. In prior laboratory studies the COX-1 C50T polymorphism was associated with an impaired inhibitory effect of aspirin on thromboxane production and platelet function. However, in this cohort of patients using low-dose aspirin for secondary prevention the polymorphism was not associated with a higher risk of atherothrombotic events.
authors.affiliation: Department of Cardiology 670, Radboud University Nijmegen MC, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. n.clappers@cardio.umcn.nl
authors.forename: Nick
authors.initials: N
authors.lastname: Clappers
authors.forename: Martijn G H
authors.initials: MG
authors.lastname: van Oijen
authors.forename: Santosh
authors.initials: S
authors.lastname: Sundaresan
authors.forename: Marc A
authors.initials: MA
authors.lastname: Brouwer
authors.forename: Rene H M
authors.initials: RH
authors.lastname: Te Morsche
authors.forename: Wessel
authors.initials: W
authors.lastname: Keuper
authors.forename: Wilbert H M
authors.initials: WH
authors.lastname: Peters
authors.forename: Joost P H
authors.initials: JP
authors.lastname: Drenth
authors.forename: Freek W A
authors.initials: FW
authors.lastname: Verheugt
mesh_terms: D000368:Aged; D001241:Aspirin / Q000008:administration & dosage*; D002318:Cardiovascular Diseases / Q000201:enzymology / Q000209:etiology / Q000401:mortality / Q000517:prevention & control*; D051545:Cyclooxygenase 1 / Q000235:genetics* / Q000378:metabolism; D016861:Cyclooxygenase Inhibitors / Q000008:administration & dosage*; D004351:Drug Resistance / Q000235:genetics; D005260:Female; D005343:Fibrinolytic Agents / Q000008:administration & dosage*; D005838:Genotype; D006801:Humans; D008297:Male; D008875:Middle Aged; D010597:Pharmacogenetics; D010641:Phenotype; D010975:Platelet Aggregation Inhibitors / Q000008:administration & dosage*; D011110:Polymorphism, Genetic; D016016:Proportional Hazards Models; D018570:Risk Assessment; D055502:Secondary Prevention; D013927:Thrombosis / Q000150:complications / Q000188:drug therapy* / Q000201:enzymology / Q000401:mortality; D017211:Treatment Failure
chemical_list: D016861:Cyclooxygenase Inhibitors; D005343:Fibrinolytic Agents; D010975:Platelet Aggregation Inhibitors; D051545:Cyclooxygenase 1; C496511:PTGS1 protein, human; D001241:Aspirin