Array and distribution of actin filaments in guard cells contribute to the determination of stomatal aperture.
Actin filaments in guard cells and their dynamics function in regulating stomatal movement. In this study, the array and distribution of actin filaments in guard cells during stomatal movement were studied with two vital labeling, microinjection of alexa-phalloidin in Vicia faba and expression of GFP-mTn in tobacco. We found that the random array of actin filaments in the most of the closed stomata changed to a ring-like array after stomatal open. And actin filaments, which were throughout the cytoplasm of guard cells of closed stomata (even distribution), were mainly found in the cortical cytoplasm in the case of open stomata (cortical distribution). These results revealed that the random array and even distribution of actin filaments in guard cells may be required for keeping the closed stomata; similarly, the ring-like array and cortical distribution of actin filaments function in sustaining open stomata. Furthermore, we found that actin depolymerization, the trait of moving stomata, facilitates the transformation of actin array and distribution with stomatal movement. So, the depolymerization of actin filaments was favorable for the changes of actin array and distribution in guard cells and thus facilitated stomatal movement.
Gao
Xin-Qi
XQ
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University 100094, Beijing, People's Republic of China.
Chen
Jing
J
Wei
Peng-Cheng
PC
Ren
Fei
F
Chen
Jia
J
Wang
Xue-Chen
XC
D008841:Actin Cytoskeleton / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008845:Microinjections; D010590:Phalloidine / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D054046:Plant Stomata / Q000166:cytology* / Q000502:physiology*; D011108:Polymers / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology; D014170:Transformation, Genetic; D031307:Vicia faba / Q000166:cytology
D010940:Plant Proteins; D011108:Polymers; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D010590:Phalloidine