[The effects of Lysophospholipase from Clonorchis sinensis on the hepatic stellate cells and oval cells of rat].
OBJECTIVE
To clarify the effects of the recombinant protein of Lysophospholipase from Clonorchis sinensis (CsLysoPLA) on the hepatic stellate cells (HSC) and oval cells of rat.


METHODS
Binding of the recombinant CslysoPLA protein to the membrane of HSC and oval cells was identified by immunofluorescent staining. The HSC and oval cells were cultured and treated with the recombinant protein at different doses, and proliferation was quantified by MTT method. Cell cycle analysis was performed by flow cytometry.


RESULTS
The recombinant CslysoPLA protein could bind to the membrane of HSC and oval cells. Compared to control, 2 mg/L and 20 mg/L the recombinant protein could promote HSC and oval cells growth (P<0.05), whereas 200 mg/L the recombinant protein could induce the cells necrosis, which associated with overt plasma membrane disruption. Oval cell number in G(2) phase of the recombinant protein 20 mg/L treated group was higher than that of control group.


CONCLUSIONS
In vitro, the recombinant protein could induce HSC and oval cells proliferation at low concentrations (2 mg/L and 20 mg/L), whereas it also could induce the cells necrosis at high concentration (200 mg/L). These results suggested that CslysoPLA might play a role in the pathogenicity of C. sinensis.
Ma
Chang-Ling
CL
Department of Microbiology and Parasitology, Guangzhou Medical College, Guangzhou 510182, China. changlingm@126.com
Hu
Xu-Chu
XC
Hu
Feng-Yu
FY
Zhou
Hong-Juan
HJ
Xue
Ling
L
Yu
Xin-Bing
XB
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003003:Clonorchiasis / Q000378:metabolism; D003004:Clonorchis sinensis / Q000201:enzymology*; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D055166:Hepatic Stellate Cells / Q000166:cytology*; D022781:Hepatocytes / Q000166:cytology* / Q000187:drug effects*; D008099:Liver / Q000166:cytology*; D008245:Lysophospholipase / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D009336:Necrosis / Q000139:chemically induced; D011485:Protein Binding; D051381:Rats; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism / Q000493:pharmacokinetics*
D011994:Recombinant Proteins; D008245:Lysophospholipase