[Impacts of tetramethylpyrazine on BDNF, bFGF expression and neuron-protection in severe brain injury tissue of rat].
OBJECTIVE
To observe the expression levels of BDNF and bFGF, the variation of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and injury neuron of rat, and to evaluate the effect of Tetramethylpyrazine (TMP) on neuro-protection finally.


METHODS
120 SD rats were randomly assigned into three groups: pseudo-operation group, severe brain injury model group and TMP treatment group. Every group contained 40 rats. The severe brain injury (SBI) of rat was established according to Feeney' s method. The treatment group was administrated with TMP transperitoneally. Rats were killed at 7 h, 24 h, 72 h, 168 h respectively after SBI. BDNF, bFGF, Nissl body and neurofunction were detected in every group at all the time scales. MDA and SOD of serum were also detected by radical immunoassay.


RESULTS
After rat SBI treated with the administration of TMP, the expression of BDNF and bFGF increased significantly in the injured brain tissue (P<0.05), and the MDA level showed to be decreased but the SOD was increased in serum (P<0.05). The Nissl body and neuro-function were well protected.


CONCLUSIONS
TMP may protect the rat cerebral neurons from SBI.
Ma
Lu
L
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Liu
Wen-Ke
WK
Zhang
Yue-Kang
YK
Ju
Yan
Y
D000818:Animals; D001930:Brain Injuries / Q000473:pathology / Q000503:physiopathology* / Q000517:prevention & control; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000096:biosynthesis*; D007150:Immunohistochemistry; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D018696:Neuroprotective Agents / Q000494:pharmacology; D011719:Pyrazines / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D013482:Superoxide Dismutase / Q000097:blood
D019208:Brain-Derived Neurotrophic Factor; D018696:Neuroprotective Agents; D011719:Pyrazines; D016222:Fibroblast Growth Factor 2; D008315:Malondialdehyde; D013482:Superoxide Dismutase; C017953:tetramethylpyrazine