title: Design and synthesis of regioisomerically pure unsymmetrical xanthene derivatives for staining live cells and their photochemical properties.
abstract: We have demonstrated the synthesis of regioisomerically pure unsymmetrical xanthene derivatives consisting of three units which can be independently modified to control their physical properties. The photochemical properties of the synthetic unsymmetrical xanthene derivatives were investigated in solution by UV-vis absorption and fluorescence measurements, and their cell imaging properties were examined by confocal laser-scanning microscopy.
authors.affiliation: Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Kamino
authors.forename: Hayato
authors.initials: H
authors.lastname: Ichikawa
authors.forename: Shun-ichi
authors.initials: S
authors.lastname: Wada
authors.forename: Yuka
authors.initials: Y
authors.lastname: Horio
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Usami
authors.forename: Takako
authors.initials: T
authors.lastname: Yamaguchi
authors.forename: Toshiki
authors.initials: T
authors.lastname: Koda
authors.forename: Aki
authors.initials: A
authors.lastname: Harada
authors.forename: Kazusa
authors.initials: K
authors.lastname: Shimanuki
authors.forename: Masao
authors.initials: M
authors.lastname: Arimoto
authors.forename: Mitsunobu
authors.initials: M
authors.lastname: Doi
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Fujita
mesh_terms: D001577:Benzophenones / Q000138:chemical synthesis* / Q000737:chemistry; D002477:Cells / Q000187:drug effects; D004396:Coloring Agents / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal; D008958:Models, Molecular; D015394:Molecular Structure; D010777:Photochemistry / Q000379:methods*; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D014407:Tumor Cells, Cultured; D014966:Xanthenes / Q000138:chemical synthesis* / Q000737:chemistry
chemical_list: D001577:Benzophenones; D004396:Coloring Agents; D005456:Fluorescent Dyes; D014966:Xanthenes