Liquid chromatography-tandem mass spectrometric quantification of the dehydration product of tetranor PGE-M, the major urinary metabolite of prostaglandin E(2) in human urine.
A LC-MS/MS method has been developed to analyze tetranor PGE-M, the major urinary metabolite of PGE(2), that involves the acid-catalyzed dehydration of tetranor PGE-M and its deuterated (d(6)) analog followed by LC-MS/MS measurement of the dehydrated tetranor PGE-M product (tetranor PGA-M). We also report a method for quantification of creatinine in urine by LC-MS/MS to normalize tetranor PGE-M concentrations with that of urinary creatinine. These methods were used to study the effect of aspirin on urinary tetranor PGE-M levels in healthy male volunteers. Aspirin did not affect urinary creatinine concentrations but decreased urinary tetranor PGE-M concentrations by approximately 44%.
Neale
Jason R
JR
Department of Drug Metabolism, Array BioPharma Inc, Boulder, CO 80301, USA. jason.neale@arraybiopharma.com <jason.neale@arraybiopharma.com>
Dean
Brian J
BJ
D000328:Adult; D001241:Aspirin / Q000494:pharmacology; D002853:Chromatography, Liquid / Q000379:methods*; D003404:Creatinine / Q000652:urine; D015232:Dinoprostone / Q000378:metabolism* / Q000652:urine; D004355:Drug Stability; D006801:Humans; D008297:Male; D011453:Prostaglandins / Q000652:urine*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*
D011453:Prostaglandins; C532049:tetranor prostaglandin M; D003404:Creatinine; D015232:Dinoprostone; D001241:Aspirin