Growth characteristic of Camel pox and Vaccinia viruses in embryonated eggs and cell culture.
Camel pox viruses isolated in Sudan and VD45 (African camel pox strain) and Vaccinia virus (Elstree strain) were used for inoculation of chorioallantoic membrane (CAM) of embryonated eggs (EE) and cell culture (CC). In EE Lesions were seen as pocks ranging in size from 1 to 1.5 mm in diameter, and they increase in size with serial passage and taking opaque- white and opaque- yellow colors. When propagated in Vero cells, these viruses gave clear CPE, characterized by rounding of cells, plaque formation, syncytia and detachment of cells from the glass.
Sheikh Ali
Hanan M
HM
Department of Microbiology, College of Veterinary Medicine & Animal Production, Sudan University of Science & Technology, P.O. Box: 204, Khartoum North, Sudan. diktora@yahoo.com
Nimir
Abdel Hafeez H
AH
Khalafalla
Abdelmalik I
AI
D000818:Animals; D002162:Camelus; D002642:Chick Embryo; D002522:Chlorocebus aethiops; D049033:Chorioallantoic Membrane / Q000821:virology*; D004531:Eggs / Q000821:virology*; D008566:Membranes / Q000821:virology; D011212:Poxviridae / Q000254:growth & development*; D014616:Vaccinia virus / Q000254:growth & development*; D014709:Vero Cells / Q000821:virology*