Characterization of a chalcosyltransferase (gerGTII) in dihydrochalcomycin biosynthesis.
An open reading frame, designated GerGTII and located downstream of the polyketide synthase genes, has been identified as a chalcosyltransferase by sequence analysis in the dihydrochalcomycin biosynthetic gene cluster of Streptomyces sp. KCTC 0041BP. The deduced product of gerGTII is similar to several glycosyltransferases, authentic and putative, and it displays a consensus sequence motif that appears to be characteristic of a sub-group of these enzymes. Specific disruption of gerGTII within the S. sp. KCTC 0041BP genome by insertional in-frame deletion method, resulted complete abolishment of dihydrochalcomycin and got the 20-O-mycinosyl-dihydrochalconolide as intermediate product in dihydrochalcomycin biosynthesis which was confirmed by electron spray ionization-mass spectrometry and liquid chromatography-mass spectrometry. Dihydrochalcomycin also was recovered after complementation of gerGTII.
Pageni
Binod Babu
BB
Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical Engineering, SunMoon University, Asan 336-708, Korea.
Oh
Tae-Jin
TJ
Thuy
Ta Thi Thu
TT
Sohng
Jae Kyung
JK
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005816:Genetic Complementation Test; D018942:Macrolides / Q000737:chemistry / Q000378:metabolism; D008969:Molecular Sequence Data; D015394:Molecular Structure; D005810:Multigene Family; D016415:Sequence Alignment; D013302:Streptomyces / Q000201:enzymology; D014166:Transferases / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D018942:Macrolides; C002018:chalcomycin; D014166:Transferases