A new murine model of allergic rhinitis by repeated intranasal Cry j 1 challenge.
To evaluate the long-lasting effects of new therapeutic approaches to allergies, we established a new model of allergic rhinitis by repeated challenges with intranasal Cry j 1, a common Japanese cedar (Cryptomeria japonica) pollen allergen, in B10.S mice. We sensitized B10.S mice subcutaneously with Cry j 1/alum three times at 1-week intervals. Five weeks after the final sensitization, we challenged the mice by instilling Cry j 1 intranasally for 5 consecutive days starting 1 day after intranasal histamine pretreatment (challenge-1). We challenged the mice by instilling histamine and Cry j 1 intranasally again 12 weeks later (challenge-2). There were significantly more sneezes after challenge-2 than challenge-1. Cry j 1-specific IgE levels in serum were significantly increased in both challenge-1 and 2 after continuous nasal antigen challenge. Serum levels of anti-Cry j 1 IgE in challenge-2 was 2.3 times higher than after challenge-1. Thus, we have established a new model of seasonal allergic rhinitis in B10.S mice by repeated intranasal antigen challenge, and this model may help elucidate mechanisms of allergic rhinitis and the development of new drugs.
Tsunematsu
Masako
M
Research and Development Center, Division of Research and Development, Meiji Dairies Corporation, Kanagawa, Japan. masako_tsunematsu@meiji-milk.com
Yamaji
Taketo
T
Kozutsumi
Daisuke
D
Murakami
Rika
R
Nagai
Hiroichi
H
Kino
Kohsuke
K
D000281:Administration, Intranasal; D000485:Allergens / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology; D000818:Animals; D000906:Antibodies / Q000097:blood; D052179:Antigens, Plant; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D051379:Mice; D010940:Plant Proteins / Q000008:administration & dosage* / Q000097:blood / Q000276:immunology; D006255:Rhinitis, Allergic, Seasonal / Q000276:immunology*; D012912:Sneezing
D000485:Allergens; D000906:Antibodies; D052179:Antigens, Plant; C065290:Cry j I protein, Cryptomeria japonica; D010940:Plant Proteins