title: [Preparation and preliminary application of rabbit anti-human PON2 antibodies(paraoxonase-2)].
abstract: OBJECTIVE
  To preparation and characterize the rabbit polyclonal antibodies against human PON2 (paraoxonase-2).
  
  
  METHODS
  A fragment of human PON2 gene which was of low homology with rabbits but of higher hydrophilicity and immunogenicity was selected for recombinant expression in prokaryotic expression system. The rabbits were immunized with the purified GST fusion protein 3 times. The specificity and sensitivity of the anti-human PON2 polyclonal antibodies were detected by Western blot and indirect immunofluorescence.
  
  
  RESULTS
  The GST-PON2 fusion protein was highly expressed in Ecoli with a molecular weight of 46 kDa. Western blot analysis proved the rabbit polyclonal antibodies could specifically recognize 39 kDa native PON2 protein expressed in several cells and tissues, such as HeLa cells, U937 cells, and human liver tissue. Indirect immunofluorescence analysis confirmed that PON2 protein was located in the cytoplasm of SY5Y cells.
  
  
  CONCLUSIONS
  The rabbit polyclonal antibodies against human PON2 can specifically recognize natural protein expressed in human cells and tissues, Which can be used for further study and clinical detection of human PON2.
authors.affiliation: College of Preclinical Medicine, Anhui Medical University, Hefei 230032, China. chenmiao_6611@163.com
authors.forename: Miao
authors.initials: M
authors.lastname: Chen
authors.forename: Jin-Ju
authors.initials: JJ
authors.lastname: Yang
authors.forename: Shu-Zhen
authors.initials: SZ
authors.lastname: Li
authors.forename: Xiao-Lan
authors.initials: XL
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.forename: Lin-Jie
authors.initials: LJ
authors.lastname: Zhang
authors.forename: Jian-En
authors.initials: JE
authors.lastname: Gao
authors.forename: Qi-Hong
authors.initials: QH
authors.lastname: Sun
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology* / Q000302:isolation & purification / Q000378:metabolism*; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D015153:Blotting, Western; D002460:Cell Line; D006367:HeLa Cells; D006801:Humans; D011817:Rabbits; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism
chemical_list: D000906:Antibodies; D011993:Recombinant Fusion Proteins; D043303:Aryldialkylphosphatase; C468411:PON2 protein, human