Evidence for replication of cauliflower mosaic virus DNA in plant nuclei.
Cellular organelles of cauliflower mosaic virus-infected turnip plants were isolated, incubated with 32P-labeled deoxyribonucleoside triphosphates and assayed for their ability to synthesize virus-specific DNA. Only nuclei were found to synthesize a labeled DNA product. This DNA migrated during agarose gel electrophoresis at the same mobility as viral open circular DNA. When purified by excision and phenol extraction from agarose gels followed by digestion with EcoRI restriction endonuclease, the DNA gave the same array of digestion products as authentic viral DNA. These results suggest that CaMV DNA may replicate in plant nuclei.
Ansa
O A
OA
Department of Plant Pathology, University of California, Davis, California 95616, USA.
Bowyer
J W
JW
Shepherd
R J
RJ