title: Characterization of EprA, a major extracellular protein of Oenococcus oeni with protease activity.
abstract: Extracellular proteins from Oenococcus oeni, a wine-making bacterium, were isolated during growth on media differing by their nitrogen content. Analysis by two-dimensional electrophoresis revealed a low number of protein signals. Among the main spots, one signal corresponded to a single protein, which contained a lysine repeat domain characteristic of cell-wall hydrolases. We demonstrated that this major protein, named EprA, was able to hydrolyse several proteins. The heterologous production of this protein in Escherichia coli confirmed the protease activity of EprA. With a MW of 21.3 kDa and a pI of 5.3, EprA presents optimal activity at pH 7.0 and 45 degrees C. This O. oeni protease differs from all lactic acid bacteria proteases so far identified, and thus this bacterium possesses at least three proteases for wine protein hydrolysis.
authors.affiliation: Laboratoire Recherche en Vigne et Vin, Institut Universitaire de la Vigne et du Vin (IUVV) Jules Guyot, Rue Claude Ladrey, Université de Bourgogne, F-21000 Dijon, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Folio
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Ritt
authors.forename: Hervé
authors.initials: H
authors.lastname: Alexandre
authors.forename: Fabienne
authors.initials: F
authors.lastname: Remize
mesh_terms: D001426:Bacterial Proteins / Q000302:isolation & purification* / Q000378:metabolism; D003470:Culture Media; D015180:Electrophoresis, Gel, Two-Dimensional; D004591:Electrophoresis, Polyacrylamide Gel; D005285:Fermentation; D005516:Food Microbiology; D006863:Hydrogen-Ion Concentration; D007934:Leuconostoc / Q000201:enzymology*; D008970:Molecular Weight; D009584:Nitrogen / Q000378:metabolism; D010447:Peptide Hydrolases / Q000302:isolation & purification / Q000378:metabolism*; D013696:Temperature; D014920:Wine / Q000382:microbiology*
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D010447:Peptide Hydrolases; D009584:Nitrogen