title: Incidence of TCR and TCL1 gene translocations and isochromosome 7q in peripheral T-cell lymphomas using fluorescence in situ hybridization.
abstract: Translocations involving the T-cell receptor (TCR) and TCL1 genes occur in T-cell precursor lymphoblastic leukemia/lymphoma and prolymphocytic leukemia; isochromosome 7q has been associated with hepatosplenic T-cell lymphoma. However, the incidence of these abnormalities in peripheral T-cell lymphomas (PTCLs) as a whole has not been well defined. We studied genetic abnormalities in 124 PTCLs seen at the Mayo Clinic, Rochester, MN, between 1987 and 2007. Tissue microarrays were screened using 2-color break-apart fluorescence in situ hybridization probes flanking the TCRalpha (TCRA, 14q11), TCRbeta (TCRB, 7q35), and TCRgamma (TCRG, 7p15) genes and the TCL1 gene (14q32). Isochromosome 7q was analyzed by using a 2-color probe to 7p and 7q32.1. Translocations involved TCRA in 3 (2.9%) of 102 cases and TCRB in 1 (1%) of 88. Isochromosome 7q was detected in 2 cases of extranodal NK/T-cell lymphoma, nasal type, and 2 cases of anaplastic lymphoma kinase-negative anaplastic large cell lymphoma. One of the latter cases also had a translocation of TCRA, and further studies confirmed a novel t(5;14) translocation.
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Feldman
authors.forename: Mark
authors.initials: M
authors.lastname: Law
authors.forename: Karen L
authors.initials: KL
authors.lastname: Grogg
authors.forename: Erik C
authors.initials: EC
authors.lastname: Thorland
authors.forename: Stephanie
authors.initials: S
authors.lastname: Fink
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Kurtin
authors.forename: William R
authors.initials: WR
authors.lastname: Macon
authors.forename: Ellen D
authors.initials: ED
authors.lastname: Remstein
authors.forename: Ahmet
authors.initials: A
authors.lastname: Dogan
mesh_terms: D000328:Adult; D000368:Aged; D002897:Chromosomes, Human, Pair 7; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D018404:Isochromosomes; D016411:Lymphoma, T-Cell, Peripheral; D008297:Male; D008875:Middle Aged; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D011948:Receptors, Antigen, T-Cell; D046888:Tissue Array Analysis; D014178:Translocation, Genetic
chemical_list: D011518:Proto-Oncogene Proteins; D011948:Receptors, Antigen, T-Cell; C090851:TCL1A protein, human