title: [The prevalence of tetracycline resistance genes in clinical and commensal strains of Enterococcus faecalis isolated in Gdańsk].
abstract: The common use of antibiotics is responsible for selecting of drug resistance not only in pathogenic, clinical bacteria but also in commensal, not pathogenic strains which could cause the rapid dissemination of the resistance to these antibacterial agents. However, information regarding the antibiotic resistance of commensal bacteria is very scarce, and the data is based mostly on phenotypical research. Therefore the use of genotyping methods for detection of tetracycline resistance genes, in commensal and medical isolates of bacteria, is essential, for understanding the spread of antibiotic resistance. In this study 24 commensal and 27 clinical isolates of Enterococcus faecalis has been screened by PCR methods for tet(M), tet(S) genes and Tn916 and Tn5397 transpozons. Subsequently, the tet(M) gene amplicones were sequenced and phylogenetic analysis was performed. We have found that the prevalence of tet(S) gene varied significantly between commensal and clinical strains. Moreover, the frequency of transpozons in clinical isolates was much higher comparing to strains isolated from healthy individuals. The phylogenetic analysis did not show significant differences between clinical and commensal strains but it could suggest that the genetic similarity between these two groups could be favourable factor for broad range spread of tet(M) gene.
authors.affiliation: Katedra Mikrobiologii , Zakład Mikrobiologii Lekarskiej AM w Gdańsku.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Jarzembowski
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Dybikowska
authors.forename: Maria
authors.initials: M
authors.lastname: Dabrowska-Szponar
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D013293:Enterococcus faecalis / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D011044:Poland; D015995:Prevalence; D013754:Tetracyclines
chemical_list: D001426:Bacterial Proteins; C083138:Tet M resistance protein, Bacteria; D013754:Tetracyclines