title: Immunohistochemistry on frozen section of sentinel lymph nodes in breast cancer with improved morphology and blocking of endogenous peroxidase.
abstract: Sentinel lymph node biopsy in the management of patients with breast cancer is the clinical practice. Peroperative examination means that more patients can be treated in a 1-step procedure. The addition of immunohistochemistry to frozen section slides improves the detection rate of especially micrometastasis. We present a novel method for immunohistochemical staining on a frozen section material that gives better morphology and blocks endogenous peroxidase sufficiently.
authors.affiliation: Department of Pathology, Vejle Hospital, Region of Southern Denmark, Denmark. anne.marie.bak.jylling@vgs.regionsyddanmark.dk
authors.forename: Anne Marie Bak
authors.initials: AM
authors.lastname: Jylling
authors.forename: Jan
authors.initials: J
authors.lastname: Lindebjerg
authors.forename: Lone
authors.initials: L
authors.lastname: Nielsen
authors.forename: Judith
authors.initials: J
authors.lastname: Jensen
mesh_terms: D000906:Antibodies; D001943:Breast Neoplasms / Q000175:diagnosis* / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D005260:Female; D005629:Frozen Sections; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000201:enzymology / Q000276:immunology / Q000473:pathology*; D008207:Lymphatic Metastasis; D009195:Peroxidase / Q000037:antagonists & inhibitors* / Q000502:physiology; D021701:Sentinel Lymph Node Biopsy
chemical_list: D000906:Antibodies; D009195:Peroxidase