title: In vivo osteoprogenitor potency of human stromal cells from different tissues does not correlate with expression of POU5F1 or its pseudogenes.
abstract: Expression of "stemness" markers is widely used as a predictor of stem cell properties of mesenchymal stem cells (MSC). Here, we show that bone marrow-derived (BM)-MSC show stem cell-like behavior in vivo; that is, they form ossicles with formation of bone, formation of adipocytes, and establishment of the murine hematopoietic microenvironment. Multipotent umbilical vein-derived stromal cells (UVSC), on the other hand, do not form bone, nor do they give rise to adipocytes in vivo. Despite these differences in stem-cell-like behavior, BM-MSC and UVSC express the two transcripts variants of POU5F1 at a similar level. Also, we found that in BM-MSC and UVSC, POU5F1 is detectable. However, more than 89% of the POU5F1 transcripts correspond to the POU5F1P1, -P3, or -P4 pseudogene. Despite low-level expression of POU5F1, we were unable to precipitate POU5F1 protein in either BM-MSC or UVSC. These results demonstrate that MSC stemness does not correlate to expression of POU5F1 transcripts or its pseudogenes.
authors.affiliation: Third Department of Internal Medicine, Klinikum rechts der Isar, Technische Universität München, München, Germany.
authors.forename: Nikolas
authors.initials: N
authors.lastname: Kaltz
authors.forename: Alessia
authors.initials: A
authors.lastname: Funari
authors.forename: Sandra
authors.initials: S
authors.lastname: Hippauf
authors.forename: Bruno
authors.initials: B
authors.lastname: Delorme
authors.forename: Danièle
authors.initials: D
authors.lastname: Noël
authors.forename: Mara
authors.initials: M
authors.lastname: Riminucci
authors.forename: Volker R
authors.initials: VR
authors.lastname: Jacobs
authors.forename: Thomas
authors.initials: T
authors.lastname: Häupl
authors.forename: Christian
authors.initials: C
authors.lastname: Jorgensen
authors.forename: Pierre
authors.initials: P
authors.lastname: Charbord
authors.forename: Christian
authors.initials: C
authors.lastname: Peschel
authors.forename: Paolo
authors.initials: P
authors.lastname: Bianco
authors.forename: Robert A J
authors.initials: RA
authors.lastname: Oostendorp
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D019070:Cell Lineage; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis* / Q000235:genetics; D010012:Osteogenesis; D011544:Pseudogenes; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
chemical_list: D000943:Antigens, Differentiation; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human