Interleukin-20 targets renal cells and is associated with chronic kidney disease.
Interleukin (IL)-10 is an anti-inflammatory factor that suppresses renal fibrosis and improves renal function in CKD rats. IL-20 belongs to the IL-10 family; therefore, we sought to determine whether IL-20 is involved in CKD. CKD patients at stage five expressed significantly higher IL-20 in serum than controls. Immunohistochemical staining demonstrated that more IL-20 protein was expressed in the kidney tubular-epithelial cells, mesangial cells, and immune cells of CKD rats with a 5/6 nephrectomy. The lung, liver, and heart tissue of CKD rats also overexpressed IL-20. Thus, we treated two tubular epithelial cells, TKPTS and M-1 cells, with IL-20 to study its effects on CKD. IL-20 treatment induced apoptosis in these cells via caspase-3 activation. Incubating IL-20 with rat interstitial fibroblasts, NRK-49F cells, upregulated TGF-beta1 production, one key inducer for renal fibrogenesis. Therefore, IL-20 injured renal epithelial cells and induced fibroblasts to produce TGF-beta1 that hastened the progression of CKD.
Wei
Chi-Chen
CC
Institute of Basic Medical Sciences, Medical College, National Cheng Kung University, 138 Sheng-Li Road, Tainan 70428, Taiwan.
Li
Hsing-Hui
HH
Hsu
Yu-Hsiang
YH
Hsing
Chung-Hsi
CH
Sung
Junne-Ming
JM
Chang
Ming-Shi
MS
D000818:Animals; D017209:Apoptosis; D002478:Cells, Cultured; D002908:Chronic Disease; D004195:Disease Models, Animal; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology; D006801:Humans; D007378:Interleukins / Q000097:blood / Q000378:metabolism*; D007674:Kidney Diseases / Q000097:blood / Q000378:metabolism* / Q000473:pathology*; D007684:Kidney Tubules / Q000378:metabolism* / Q000473:pathology*; D008099:Liver / Q000378:metabolism / Q000473:pathology; D008168:Lung; D008297:Male; D051379:Mice; D009206:Myocardium; D051381:Rats; D017207:Rats, Sprague-Dawley; D014018:Tissue Distribution; D053773:Transforming Growth Factor beta1 / Q000378:metabolism
D007378:Interleukins; D053773:Transforming Growth Factor beta1; C422042:interleukin 20