Studies on phage development. II. The maturation of T4 phage in the presence of puromycin.
Maturation of T4 can proceed in the presence of 5-methyltryptophan or puromycin. Puromycin inhibits phage protein synthesis within a few seconds, thus limiting the amount of phage precursor material available for maturation. After the arrest of protein synthesis, maturation continues unabated until the limiting protein precursor is depleted. At least one protein, the tail fiber protein, is depleted. Phage protein maturable in the presence of puromycin appears about 5 minutes before maturation begins. The level of the maturable material reaches a maximum at the beginning of maturation. This level is taken as a measure of the size of the pool of complete sets of maturable phage protein. The bulk precursor protein begins to accumulate 3 minutes earlier than serum blocking proteins (SBP) and forms a pool about twice as large as the pool of SBP.
Piechowski
M M
MM
Department of Genetics, University of Wisconsin, Madison, Wisconsin, USA.
Susman
M
M