title: Increasing cell adhesion on plasma deposited fluorocarbon coatings by changing the surface topography.
abstract: In designing new biomaterials, it is of outstanding importance to consider how cells respond to specific chemical and topographical features on the material surface. The behavior of most cell types in vivo is strictly related to specific chemical and topographical cues that characterize the extra cellular environment. In particular, during their lives cells react to topographical patterns such as those of the extracellular matrix (ECM), of micro and/or nanometric dimensions. The production of micrometric and/or nanometric features on artificial materials usually involves expensive and time-consuming methods of manufacturing, such as electron beam and colloidal lithography. In this article, different "Teflon-like" structured surfaces were deposited from tetrafluoroethylene (C(2)F(4))-fed plasmas, for the study of cell adhesion and growth. The reaction of different cell lines to different topographical features was evaluated and compared with cell behavior on flat samples with the same chemical composition. Cell adhesion was calculated from area covered by cells at different time of culture. Beside this, cell proliferation was determined with the MTT test. Cell morphology and filopodia interaction with the nanofeatures were also estimated by optical and scanning electron microscopy. A dramatic difference both in adhesion and growth was found between cells seeded on flat and rough surfaces with the density and spreading of adhered cells varying as a function of the roughness of coatings.
authors.affiliation: Institute of Inorganic Methodologies and Plasmas (IMIP), CNR Bari, 7016 Bari, Italy. gristina@chimica.uniba.it
authors.forename: Roberto
authors.initials: R
authors.lastname: Gristina
authors.forename: Erica
authors.initials: E
authors.lastname: D'Aloia
authors.forename: Giorgio S
authors.initials: GS
authors.lastname: Senesi
authors.forename: Antonella
authors.initials: A
authors.lastname: Milella
authors.forename: Marina
authors.initials: M
authors.lastname: Nardulli
authors.forename: Eloisa
authors.initials: E
authors.lastname: Sardella
authors.forename: Pietro
authors.initials: P
authors.lastname: Favia
authors.forename: Riccardo
authors.initials: R
authors.lastname: d'Agostino
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D002448:Cell Adhesion; D002462:Cell Membrane / Q000378:metabolism; D003102:Colloids / Q000737:chemistry; D005109:Extracellular Matrix / Q000378:metabolism; D005461:Fluorine / Q000737:chemistry*; D005466:Fluorocarbons / Q000737:chemistry; D006801:Humans; D051379:Mice; D008855:Microscopy, Electron, Scanning / Q000379:methods; D013499:Surface Properties; D013778:Tetrazolium Salts / Q000737:chemistry; D013844:Thiazoles / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D003102:Colloids; D005466:Fluorocarbons; D013778:Tetrazolium Salts; D013844:Thiazoles; D005461:Fluorine; D002244:Carbon; C022616:thiazolyl blue; C015531:tetrafluoroethylene