title: Physical map of bacteriophage phi29 DNA.
abstract: Restriction endonuclease EcoRI cleaves linear phi29 DNA at four points yielding five fragments (A-E) of molecular weights 6.0 x 10(6), 3.8 x 10(6), 1.3 x 10(6), 0.6 x 10(6), and 0.3 x 10(6). The relative order of the fragments was shown to be A, B, E, D, C by analysis of the EcoRI partial digestion products and from the study of the fragments obtained by treatment of protein-containing DNA with EcoRI and trypsin. The EcoRI cleavage map of phi29 DNA has been ordered relative to the genetic map by marker rescue experiments.
authors.affiliation: Centro de Biologia Molecular (C.S.I.C.-U.A.M.), Velázquez 144, Madrid-6, Spain.
authors.forename: M R
authors.initials: MR
authors.lastname: Inciarte
authors.forename: J
authors.initials: J
authors.lastname: Lázaro
authors.forename: M
authors.initials: M
authors.lastname: Salas
authors.forename: E
authors.initials: E
authors.lastname: Viñuela