Tomatinase from Fusarium oxysporum f. sp. lycopersici is required for full virulence on tomato plants.
Saponin detoxification enzymes from pathogenic fungi are involved in the infection process of their host plants. Fusarium oxysporum f. sp lycopersici, a tomato pathogen, produces the tomatinase enzyme Tom1, which degrades alpha-tomatine to less toxic derivates. To study the role of the tom1 gene in the virulence of F. oxysporum, we performed targeted disruption and overexpression of the gene. The infection process of tomato plants inoculated with transformants constitutively producing Tom1 resulted in an increase of symptom development. By contrast, tomato plants infected with the knockout mutants showed a delay in the disease process, indicating that Tom1, although not essential for pathogenicity, is required for the full virulence of F. oxysporum. Total tomatinase activity in the disrupted strains was reduced only 25%, leading to beta(2)-tomatine as the main hydrolysis product of the saponin in vitro. In silico analysis of the F. oxysporum genome revealed the existence of four additional putative tomatinase genes with identities to tomatinases from family 3 of glycosyl hydrolases. These might be responsible for the remaining tomatinase activity in the Deltatom1 mutants. Our results indicate that detoxification of alpha-tomatine in F. oxysporum is carried out by several tomatinase activities, suggesting the importance of these enzymes during the infection process.
Pareja-Jaime
Yolanda
Y
Departamento de Genética, Universidad de Córdoba, Edificio Gregor Mendel, Campus de Rabanales, 14071 Córdoba, Spain.
Roncero
M Isabel G
MI
Ruiz-Roldán
M Carmen
MC
D002855:Chromatography, Thin Layer; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D005670:Fusarium / Q000201:enzymology* / Q000235:genetics / Q000472:pathogenicity; D015971:Gene Expression Regulation, Enzymologic; D015966:Gene Expression Regulation, Fungal; D016681:Genome, Fungal; D006026:Glycoside Hydrolases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D018551:Solanum lycopersicum / Q000382:microbiology*; D009154:Mutation; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014053:Tomatine / Q000031:analogs & derivatives / Q000378:metabolism; D014774:Virulence / Q000235:genetics
D005656:Fungal Proteins; D007527:Isoenzymes; C484751:alpha-tomatine; D014053:Tomatine; D006026:Glycoside Hydrolases; C099475:tomatinase