Enzymatic N-methylaminoacylation of tRNA using chemically misacylated AMP as a substrate.
Using "chemically misacylated AMP" which is photogeneratable, tRNA can be enzymatically acylated with N-methylamino acids in an efficiency comparable to that of the corresponding natural aminoacylation process with amino acids.
Sando
Shinsuke
S
Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Kyoto, Japan. ssando@sbchem.kyoto-u.ac.jp
Masu
Hiroki
H
Furutani
Chika
C
Aoyama
Yasuhiro
Y
D000215:Acylation; D000217:Acyltransferases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000249:Adenosine Monophosphate / Q000031:analogs & derivatives / Q000737:chemistry* / Q000378:metabolism; D000595:Amino Acid Sequence; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli / Q000201:enzymology; D008969:Molecular Sequence Data; D015394:Molecular Structure; D009842:Oligopeptides / Q000737:chemistry / Q000235:genetics; D012346:RNA, Transfer, Amino Acyl / Q000737:chemistry* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D009842:Oligopeptides; D012346:RNA, Transfer, Amino Acyl; D000249:Adenosine Monophosphate; D000217:Acyltransferases