The Halogen assay--a new technique for measuring airborne allergen.
The Halogen assay is a new technique for measuring airborne allergen. The assay is unique in that it is capable of analyzing allergens and particles together, combining the advantages of morphological approaches and immunoassay. The Halogen assay allows direct observation of the particles that carry the allergen as well as being capable of identifying all the allergen sources an individual is exposed and sensitized to. The assay is sensitive because the extracted allergen is bound to the membrane at a high local concentration within the minute area around each particle and so is easily detected by immunostaining. It is therefore easy to detect few pollen grains. The Halogen method supersedes other methods commonly used to identify allergens as it is capable of identifying airborne particles that are allergen sources.
Tovey
Euan
E
Department of Medicine, University of Sydney, Australia.
Lucca
Sandra De
SD
Poulos
Leanne
L
O'Meara
Tim
T
D000393:Air Pollutants / Q000737:chemistry* / Q000302:isolation & purification; D000485:Allergens / Q000737:chemistry* / Q000302:isolation & purification; D000911:Antibodies, Monoclonal / Q000032:analysis; D006219:Halogens / Q000737:chemistry*; D007124:Immunoenzyme Techniques / Q000191:economics / Q000379:methods*; D008567:Membranes, Artificial; D011485:Protein Binding; D013048:Specimen Handling; D013194:Staining and Labeling
D000393:Air Pollutants; D000485:Allergens; D000911:Antibodies, Monoclonal; D006219:Halogens; D008567:Membranes, Artificial