Reporter genes for real-time in vive monitoring of Ochrobactrum anthropi infection.
Despite the increasing interest in Ochrobactrum anthropi as an emerging nosocomial pathogen resistant to most commonly used antimicrobials, relatively little is known about the pathogenesis and factors contributing to its virulence. Also, many aspects of interaction between Ochrobactrum spp. and their hosts remain unclear. The ability to monitor O. anthropi infection in the host will facilitate our understanding of the pathogenic mechanisms and will lead to better choices of antimicrobial or additional therapeutic strategies. We have demonstrated the ability to stably express three reporter genes (green fluorescence protein GFP, red fluorescence protein RFP and luciferase Lux) and track the infection in a J774A.1 murine macrophage cell line as well as in the BALB/c mouse. Our results suggest that these reporter genes should improve genetic studies in O. anthropi, particularly those aimed at understanding pathogenesis, virulence factors and host interaction.
Seleem
Mohamed N
MN
Institute for Critical Technology and Applied Science, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Ali
Mohammed
M
Boyle
Stephen M
SM
Sriranganathan
Nammalwar
N
D000818:Animals; D002460:Cell Line; D017930:Genes, Reporter; D016905:Gram-Negative Bacterial Infections / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D006801:Humans; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008264:Macrophages / Q000382:microbiology; D051379:Mice; D008807:Mice, Inbred BALB C; D020621:Ochrobactrum anthropi / Q000166:cytology / Q000235:genetics / Q000502:physiology*
D008164:Luminescent Proteins