Mapping of the coat protein gene of cauliflower mosaic virus by its expression in a prokaryotic system.
Polypeptides recognized by antisera to cauliflower mosaic virus (CaMV) coat protein were found in lysates of Escherichia coli cells that carry the complete CaMV genome on plasmid vectors. Synthesis of this cross-reacting material in E. coli was detected when the complete CaMV DNA sequence was present at either of two sites and in either orientation at one site of the cloning vector, suggesting prokaryotic promoter activity for some sequence within the viral DNA. Using an immunosorbent assay to screen subclones containing various segments of the CaMV genome, open reading region IV alone was found to be sufficient for synthesis of the antigen, thereby mapping the coat protein gene to this region.
Daubert
S
S
Department of Plant Pathology, University of California, Davis, California 95616, USA.
Richins
R
R
Shepherd
R J
RJ
Gardner
R C
RC