On the origin of the polyhedral protein of Autographa californica nuclear polyhedrosis virus. Isolation, characterization, and translation of viral messenger RNA.
Virus-specific messenger RNA was isolated 24 hr postinfection from Spodoptera frugiperda cells infected with Autographa californica nuclear polyhedrosis virus (AcNPV) by means of hybridization with viral DNA that was coupled to cellulose. Viral mRNA was analyzed on isokinetic sucrose gradients and on polyacrylamide gels containing 98% formamide. Viral mRNA was also translated in an in vitro protein-synthesizing system derived from wheat germ. One product comigrated with purified AcNPV polyhedral protein (MW 30,000) and precipitated with antibodies against AcNPV polyhedral protein. These results favor the hypothesis that the polyhedral protein is a virus-coded protein.
van der Beek
C P
CP
Department of Virology, Agricultural University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands.
Saaijer-Riep
J D
JD
Vlak
J M
JM