Microcin J25 induces the opening of the mitochondrial transition pore and cytochrome c release through superoxide generation.
Microcin J25, an antimicrobial lasso-structure peptide, induces the opening of mitochondrial permeability transition pores and the subsequent loss of cytochrome c. The microcin J25 effect is mediated by the stimulation of superoxide anion overproduction. An increased uptake of calcium is also involved in this process. Additional studies with superoxide dismutase, ascorbic acid and different specific inhibitors, such as ruthenium red, cyclosporin A and Mn(2+), allowed us to establish a time sequence of events starting with the binding of microcin J25, followed by superoxide anion overproduction, opening of mitochondrial permeability transition pores, mitochondrial swelling and the concomitant leakage of cytochrome c.
Niklison Chirou
María
M
Departamento de Bioquímica de la Nutrición, Instituto Superior de Investigaciones Biológicas, Instituto de Química Biológica, Dr Bernabe Bloj, San Miguel de Tucumán, Argentina.
Bellomio
Augusto
A
Dupuy
Fernando
F
Arcuri
Beatriz
B
Minahk
Carlos
C
Morero
Roberto
R
D000818:Animals; D000900:Anti-Bacterial Agents / Q000378:metabolism / Q000494:pharmacology*; D001430:Bacteriocins / Q000378:metabolism / Q000494:pharmacology*; D045304:Cytochromes c / Q000378:metabolism*; D008928:Mitochondria / Q000187:drug effects*; D008929:Mitochondria, Heart / Q000187:drug effects / Q000378:metabolism; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism*; D000083162:Mitochondrial Permeability Transition Pore; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D051381:Rats; D017208:Rats, Wistar; D013481:Superoxides / Q000378:metabolism*
D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D013481:Superoxides; C020411:microcin; D009249:NADP; D045304:Cytochromes c