[The fabrication of hepatitis B electrochemical immunosensor array].
Based on integrate circuit (IC) technology, an eight-channel gold electrodes (GEs) array was developed. The immunosensor array is prepared by co-immobilizing thionine and Hepatitis B (HB) antibody on the gold electrodes through covalently binding them to GEs with a cysteamine/glutaraldehyde linkage. Hepatitis B surface antigen (HBsAg) was detected qualitatively and quantitatively by the peak current decrease percentage of the thionine. HBsAg positive/negative standard serum was well defined by the array. 8-channel synchronous detection for HBsAg was noted to be of good accuracy and reliability. The results of its clinical application were in good agreement with the results from ELISA.
Dai
Xiaofeng
X
General Hospital of Guangzhou Command, Guangzhou 510010, China.
Liu
Zhongming
Z
D015374:Biosensing Techniques / Q000295:instrumentation*; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D006510:Hepatitis B Antibodies / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods
D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens