DsbB is required for the pathogenesis process of Xanthomonas campestris pv. campestris.
The DsbA/DsbB oxidation pathway is one of the two pathways that catalyze disulfide bond formation of proteins in the periplasm of gram-negative bacteria. It has been demonstrated that DsbA is essential for multiple virulence factors of several animal bacterial pathogens. In this article, we present genetic evidence to show that the open reading frame XC_3314 encodes a DsbB protein that is involved in disulfide bond formation in periplasm of Xanthomonas campestris pv. campestris, the causative agent of crucifer black rot disease. The dsbB mutant of X. campestris pv. campestris exhibited attenuation in virulence, hypersensitive response, cell motility, and bacterial growth in planta. Furthermore, mutation in the dsbB gene resulted in ineffective type II and type III secretion systems as well as flagellar assembly. These findings reveal that DsbB is required for the pathogenesis process of X. campestris pv. campestris.
Jiang
Bo-Le
BL
College of Life Science and Technology, Guangxi University, China.
Liu
Jiao
J
Chen
Li-Feng
LF
Ge
Ying-Ying
YY
Hang
Xiao-Hong
XH
He
Yong-Qiang
YQ
Tang
Dong-Jie
DJ
Lu
Guang-Tao
GT
Tang
Ji-Liang
JL
D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000235:genetics*; D003545:Cysteine / Q000378:metabolism; D005407:Flagella / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D017930:Genes, Reporter; D008565:Membrane Proteins / Q000235:genetics*; D008969:Molecular Sequence Data; D009154:Mutation; D016366:Open Reading Frames; D019897:Periplasm / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology*; D010957:Plasmids; D031224:Raphanus / Q000382:microbiology; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D014774:Virulence / Q000235:genetics; D016959:Xanthomonas campestris / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*
D001426:Bacterial Proteins; C079503:DsbB protein, Bacteria; D008565:Membrane Proteins; D003545:Cysteine