Replacement of dietary saturated FAs by PUFAs in diet and reverse cholesterol transport.
Dietary intervention is the first and usually successful approach in the treatment of high LDL cholesterol (LDL-C) concentration, but it is frequently accompanied by a decrease in HDL concentration. We studied 14 male volunteers on two different diets, high saturated fatty acid (SFA) and high PUFA, in a crossover design to test whether a decrease in HDL can affect reverse cholesterol transport from relabeled macrophages. A significant decrease of LDL-C (in mmol/l) after a PUFA diet compared with an SFA diet from 3.15 +/- 0.65 to 2.80 +/- 0.56 (P < 0.01) was accompanied by a significant decrease of HDL cholesterol (HDL-C) (in mmol/l) from 1.21 +/- 0.30 to 1.10 +/- 0.32 (P < 0.05). These changes did not affect cholesterol efflux (CHE) from macrophages (9.74 +/- 1.46% vs. 9.53 +/- 1.41%). There was no correlation between individual changes of HDL-C and changes of CHE. It is concluded that the decrease of HDL-C after successful dietary intervention of LDL-C is not accompanied by a decrease of CHE.
Kralova Lesna
I
I
Institute for Clinical and Experimental Medicine, Center for Cardiovascular Research, Prague, Czech Republic. ivka@ikem.cz
Suchanek
P
P
Kovar
J
J
Stavek
P
P
Poledne
R
R
D000293:Adolescent; D000328:Adult; D001693:Biological Transport, Active / Q000502:physiology; D045744:Cell Line, Tumor; D002784:Cholesterol / Q000378:metabolism*; D018592:Cross-Over Studies; D004032:Diet; D004041:Dietary Fats / Q000378:metabolism*; D005231:Fatty Acids, Unsaturated / Q000378:metabolism*; D006801:Humans; D008297:Male; D008875:Middle Aged; D055815:Young Adult
D004041:Dietary Fats; D005231:Fatty Acids, Unsaturated; D002784:Cholesterol