[Optimizing extraction process of Scutellaria barbata by golden section method].
OBJECTIVE
To probe into the feasibility of golden section method in the optimization of the extraction process of Scutellaria barbata.


METHODS
With the determination of the content of scutellarin and total flavonoids, selecting the range of alcohol concentration in the extraction of S. barbata was determined by the golden section method and method of equal interval, and validated it by orthogonal design.


RESULTS
The best extraction technique is that extract 3 times with 15 times of the alcohol which concentration is 70%, and each time for 1 h.


CONCLUSIONS
Applying golden section method in the extraction of S. barbata is practical, which as a method of preliminary trial, indicates that golden section method can also be applied into the field of optimization of extraction process of traditional Chinese medicine.
Liu
Jun-Yan
JY
College of Chinese Pharmacy, Beijing University of Traditional Chinese Medicine, Beijing 100102, China. 24liujunyan@yahoo.com.cn
Wang
Yu-Rong
YR
Liu
Yi
Y
D000438:Alcohols / Q000737:chemistry; D047310:Apigenin / Q000032:analysis; D002138:Calibration; D005591:Chemical Fractionation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D005240:Feasibility Studies; D005419:Flavonoids / Q000032:analysis; D005965:Glucuronates / Q000032:analysis; D010936:Plant Extracts / Q000032:analysis / Q000302:isolation & purification*; D031363:Scutellaria / Q000737:chemistry*; D013997:Time Factors; D014466:Ultraviolet Rays
D000438:Alcohols; D004365:Drugs, Chinese Herbal; D005419:Flavonoids; D005965:Glucuronates; D010936:Plant Extracts; C524947:Scutellaria barbata extract; C484876:scutellarin; D047310:Apigenin