title: Utilization of membrane vesicle preparations to study drug-ABC transporter interactions.
abstract: BACKGROUND
  The last 15 years have marked an expansion in our understanding of how ABC transporters modulate the pharmacokinetic properties of drugs. Assays based on different membrane preparations were one of the first methods developed to study ABC transporters. Later, they turned out to be valuable tools to gain insight into the nature of drug-ABC transporter interactions.
  
  
  OBJECTIVE
  Membranes prepared from different sources have been used and characterized; based on the biochemical characteristics of the transport process, a number of different assay types have been developed.
  
  
  METHODS
  This review focuses on the current experiences on how different membrane-based assays can be utilized in pharmaceutical R&D. Sources of membrane preparations, available assay types and correlation studies between different in-vitro and in-vivo methods are discussed.
  
  
  CONCLUSIONS
  Membrane-based assays are valuable tools in drug discovery to characterize drug-ABC transporter interactions.
authors.affiliation: Solvo Biotechnology, Gyár u 2, Budaörs, 2040, Hungary.
authors.forename: Hristos
authors.initials: H
authors.lastname: Glavinas
authors.forename: Dóra
authors.initials: D
authors.lastname: Méhn
authors.forename: Márton
authors.initials: M
authors.lastname: Jani
authors.forename: Berend
authors.initials: B
authors.lastname: Oosterhuis
authors.forename: Krisztina
authors.initials: K
authors.lastname: Herédi-Szabó
authors.forename: Péter
authors.initials: P
authors.lastname: Krajcsi
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000187:drug effects* / Q000378:metabolism; D000255:Adenosine Triphosphate / Q000378:metabolism; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D001692:Biological Transport / Q000187:drug effects; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D011994:Recombinant Proteins / Q000187:drug effects; D015203:Reproducibility of Results; D013347:Subcellular Fractions
chemical_list: D018528:ATP-Binding Cassette Transporters; D011994:Recombinant Proteins; D000255:Adenosine Triphosphate