title: Association study of CCR5 delta 32 polymorphism among the HLA-DRB1 Caucasian population in Northern Paraná, Brazil.
abstract: Chemokines are important determinants of early inflammatory response. The CC chemokine receptor 5 (CCR5) delta 32 variant results in a nonfunctional form of the chemokine receptor and has been implicated in a variety of immune-mediated diseases. In the present study, polymerase chain reaction (PCR) for genomic deoxyribonucleic acid (DNA) samples, using specific CCR5 oligonucleotide primers surrounding the breakpoint deletion, detected a 225-basepair (bp) product from the normal CCR5 allele and a 193-bp product from the 32 bp deletion allele. Human leukocyte antigen (HLA) class II (DRB1) typing was performed by PCR-sequence-specific primer (PCR-SSP). The aim of this study was to evaluate the association of HLA-DRB1 and CCR5 genetic polymorphisms. To evaluate the frequency distributions of CCR5 delta 32 polymorphisms in a Brazilian population and their association with allelic distribution of HLA genes, DRB1; a total of 120 Caucasian individuals from northern Paraná, Brazil, were tested. The CCR5/CCR5 genotype was found in 108 individuals (90%) and only one carried the CCR5 delta 32 allele homozygous genotype (0.0238), while 12 (10%) carried the CCR5 delta 32 allele heterozygous genotype. The observed frequency for the CCR5 delta 32 allele was 0.05 in the population studied. The results revealed a CCR5 delta 32 allele occurrence with HLA-DRB1(*)01 and DRB1(*)04 (P<0.05). It is possible that HLA-DRB1(*)01 and DRB1(*)04 alleles could be associated with the delta 32-bp deletion of CCR5.
authors.affiliation: Department of Pathological Sciences, State University of Londrina, Londrina, PR, Brazil.
authors.forename: Sandra Marcia
authors.initials: SM
authors.lastname: Muxel
authors.forename: Sueli Donizete
authors.initials: SD
authors.lastname: Borelli
authors.forename: Marla Karine
authors.initials: MK
authors.lastname: Amarante
authors.forename: Julio Cesar
authors.initials: JC
authors.lastname: Voltarelli
authors.forename: Mateus Nobrega
authors.initials: MN
authors.lastname: Aoki
authors.forename: Carlos Eduardo Coral
authors.initials: CE
authors.lastname: de Oliveira
authors.forename: Maria Angelica
authors.initials: MA
authors.lastname: Ehara Watanabe
mesh_terms: D000483:Alleles; D001938:Brazil / Q000453:epidemiology; D005787:Gene Frequency; D005820:Genetic Testing; D005828:Genetics, Population; D006684:HLA-DR Antigens / Q000235:genetics* / Q000276:immunology; D059811:HLA-DRB1 Chains; D006801:Humans; D016133:Polymerase Chain Reaction; D018807:Polymorphism, Single-Stranded Conformational; D019713:Receptors, CCR5 / Q000235:genetics*; D044465:White People / Q000235:genetics*
chemical_list: D006684:HLA-DR Antigens; D059811:HLA-DRB1 Chains; D019713:Receptors, CCR5