title: Royal jelly peptides inhibit lipid peroxidation in vitro and in vivo.
abstract: Royal jelly peptides (RJPx) isolated from hydrolysates of water-soluble royal jelly proteins prepared with protease P exhibited significantly stronger hydroxyl radical-scavenging activity (p<0.001), and antioxidant activity against lipid peroxidation (LPO, p<0.001), than did water-soluble royal jelly protein (WSRJP) in vitro. We also investigated the in vivo antioxidant activity of RJPx against ferric nitrilotriacetate (Fe-NTA)-induced LPO. Male Wistar rats were divided into a control group (Group C), an Fe-NTA group (Group Fe), and an Fe-NTA with RJPx group (Group Fe+R). Rats in Group Fe+R were fed RJPx (2 g/kg body weight) daily for 5 wk. Fe-NTA (8 mg Fe/kg body weight) was then intraperitoneally injected, and serum lipid levels were examined 2 h later. Serum total cholesterol (TC) levels were lower (p<0.05) while low-density lipoprotein (LDL) and LPO were significantly higher (p<0.01) in Group Fe than in Group C. TC (p<0.05) and LPO levels (p<0.01) were lower in Group Fe+R than in Group Fe. Our data suggest that RJPx may inhibit LPO both in vitro and in vivo.
authors.affiliation: R & D Center, Nippon Meat Packers, Inc., Tsukuba, Ibaraki, Japan. h.guo@nipponham.co.jp
authors.forename: Hang
authors.initials: H
authors.lastname: Guo
authors.forename: Ai
authors.initials: A
authors.lastname: Ekusa
authors.forename: Koji
authors.initials: K
authors.lastname: Iwai
authors.forename: Masami
authors.initials: M
authors.lastname: Yonekura
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Takahata
authors.forename: Fumiki
authors.initials: F
authors.lastname: Morimatsu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002273:Carcinogens / Q000633:toxicity; D002784:Cholesterol / Q000097:blood; D005227:Fatty Acids / Q000737:chemistry*; D005290:Ferric Compounds / Q000633:toxicity; D016166:Free Radical Scavengers / Q000494:pharmacology; D006868:Hydrolysis; D015227:Lipid Peroxidation / Q000187:drug effects*; D008055:Lipids / Q000097:blood; D008297:Male; D009571:Nitrilotriacetic Acid / Q000031:analogs & derivatives / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D000099219:Royal Jelly
chemical_list: D000975:Antioxidants; D002273:Carcinogens; D002784:Cholesterol; D005227:Fatty Acids; D005290:Ferric Compounds; D016166:Free Radical Scavengers; D008055:Lipids; D009571:Nitrilotriacetic Acid; D010455:Peptides; D000099219:Royal Jelly; C020326:ferric nitrilotriacetate