title: MVPlex assay for direct detection of methicillin-resistant Staphylococcus aureus in naris and other swab specimens.
abstract: We evaluated the MVPlex assay (Geneco Biomedical Products), which uses target-enriched multiplex PCR amplification followed by liquid array identification, for the detection of methicillin-resistant Staphylococcus aureus (MRSA) from 307 dual-swab specimens. By using a combination of culture (Trypticase soy agar-5% sheep blood agar and Columbia CNA agar-5% sheep blood) and an FDA-approved MRSA PCR assay as the "gold standard," the MVPlex MRSA assay and culture were found to have sensitivities of 97.8% and 84.4% (P = 0.002) and specificities of 95.8% and 98.6% (P < 0.05), respectively.
authors.affiliation: Department of Pathology, Waukesha Memorial Hospital, Waukesha, Wisconsin 53188, USA. raymond.podzorski@phci.org
authors.forename: Raymond P
authors.initials: RP
authors.lastname: Podzorski
authors.forename: Haijing
authors.initials: H
authors.lastname: Li
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Tang
mesh_terms: D003428:Cross Infection / Q000175:diagnosis; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus; D009297:Nasal Mucosa / Q000382:microbiology; D016133:Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013203:Staphylococcal Infections / Q000175:diagnosis*