Application of probe electrospray to direct ambient analysis of biological samples.
Recently, we have developed probe electrospray ionization (PESI) that uses a solid needle. In this system, the probe needle moves up and down along the vertical axis by a motor-driven system. At the highest position of the probe needle, electrospray is generated by applying a high voltage. In this study, we applied PESI directly to biological samples such as urine, mouse brain, mouse liver, salmon egg, and fruits (orange, banana, etc.). Strong ion signals for almost all the samples were obtained. The amount of liquid sample picked up by the needle is as small as pL or less, making PESI a promising non-invasive technique for detecting biomolecules in living systems such as cells. Therefore, PESI may be useful as a versatile and ready-to-use semi-online analytical tool in the fields of medicine, pharmaceuticals, agriculture, food science, etc.
Chen
Lee Chuin
LC
Clean Energy Research Center, University of Yamanashi, Takeda- 4, Kofu 400-8511, Japan.
Nishidate
Kentaro
K
Saito
Yuta
Y
Mori
Kunihiko
K
Asakawa
Daiki
D
Takeda
Sen
S
Kubota
Takeo
T
Terada
Nobuo
N
Hashimoto
Yutaka
Y
Hori
Hirokazu
H
Hiraoka
Kenzo
K
D000818:Animals; D001695:Biology / Q000379:methods*; D001923:Brain Chemistry; D001942:Breast Feeding; D002417:Cattle; D032084:Citrus sinensis / Q000737:chemistry; D004531:Eggs / Q000032:analysis; D005260:Female; D006096:Gramicidin / Q000032:analysis; D006801:Humans; D007328:Insulin / Q000032:analysis; D008099:Liver / Q000737:chemistry; D051379:Mice; D008892:Milk / Q000737:chemistry; D008895:Milk, Human / Q000737:chemistry; D028521:Musa / Q000737:chemistry; D012474:Salmon / Q000033:anatomy & histology; D012680:Sensitivity and Specificity; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods*; D014556:Urine / Q000737:chemistry
D007328:Insulin; D006096:Gramicidin