title: PLGA microsphere construct coated with TGF-beta 3 loaded nanoparticles for neocartilage formation.
abstract: Polymeric microsphere system has been widely used in tissue-regeneration matrix and drug delivery systems. To apply these biomaterials as novel cell supporting matrix for stem cell delivery, we have devised a novel method for the fabrication of nanostructured 3D scaffolds that growth factor loaded heparin/poly(L-lysine) nanoparticles were physically attached on the positively charged surface of PLGA microspheres precoated with low molecular weight of poly(ethyleneimmine) (PEI) via a layer-by-layer (LbL) system. Based on a previous study, we have prepared poly(lactide-co-glycolide) (PLGA) microspheres harboring heparin/poly(L-lysine) loaded with growth factors. Growth factor loaded heparin/poly(L-lysine) nanoparticles, which were simply produced as polyion complex micelles (PICM) with diameters of 50-150 nm, were fabricated in the first step. Microsphere matrix (size, 20 approximately 80 nm) containing TGF-beta 3 showed a significantly higher number of specific lacunae phenotypes at the end of the 4 week study in vitro culture of mesenchymal stem cells. Thus, growth factor delivery of PLGA microsphere can be used to engineer synthetic extracellular matrix. This PLGA microsphere matrix containing TGF-beta 3 showed promise as coatings for implantable biomedical devices to improve biocompatibility and ensure in vivo performance.
authors.affiliation: Pochon CHA University, CHA Stem Cell Institute 606-16, Yeoksam 1-dong, Seoul, Korea.
authors.forename: Ji Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Kyeongsoon
authors.initials: K
authors.lastname: Park
authors.forename: Dae Gyun
authors.initials: DG
authors.lastname: Woo
authors.forename: Han Na
authors.initials: HN
authors.lastname: Yang
authors.forename: Hyung-Min
authors.initials: HM
authors.lastname: Chung
authors.forename: Keun-Hong
authors.initials: KH
authors.lastname: Park
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002356:Cartilage / Q000378:metabolism* / Q000473:pathology; D007700:Kinetics; D019344:Lactic Acid / Q000737:chemistry; D059630:Mesenchymal Stem Cells / Q000166:cytology; D008823:Micelles; D008863:Microspheres; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000379:methods; D011094:Polyethyleneimine / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D051381:Rats; D033581:Stem Cell Transplantation; D053782:Transforming Growth Factor beta3 / Q000378:metabolism*
chemical_list: D001672:Biocompatible Materials; D008823:Micelles; D053782:Transforming Growth Factor beta3; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011094:Polyethyleneimine