title: Hsp90n - An accidental product of a fortuitous chromosomal translocation rather than a regular Hsp90 family member of human proteome.
abstract: Human cells express two isoforms of the Hsp90 protein, called Hsp90alpha and Hsp90beta. Although existence of the third form called Hsp90alphaDeltaN, or Hsp90N was reported in 1998, our investigation, based on the sequence analysis and attempts to reproduce previous results, demonstrate that there is no evidence that Hsp90N gene is present in human genome and no homologs of such a protein are present in other known eukaryotic genomes. We propose that Hsp90N was created as an artifact of a cDNA synthesis or that it is a chimeric protein, being a result of the chromosomal rearrangement that occurred in a single cell line, after this line was established.
authors.affiliation: International Institute of Molecular and Cell Biology, Department of Molecular Biology, 4 Ks. Trojdena Street, 02-109 Warsaw, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Zurawska
authors.forename: Jakub
authors.initials: J
authors.lastname: Urbanski
authors.forename: Pawel
authors.initials: P
authors.lastname: Bieganowski
mesh_terms: D000595:Amino Acid Sequence; D016477:Artifacts; D001483:Base Sequence; D002460:Cell Line; D018076:DNA, Complementary / Q000096:biosynthesis; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D005810:Multigene Family / Q000235:genetics; D050505:Mutant Proteins / Q000235:genetics; D020543:Proteome / Q000235:genetics / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D014178:Translocation, Genetic / Q000502:physiology*
chemical_list: D018076:DNA, Complementary; D018841:HSP90 Heat-Shock Proteins; D050505:Mutant Proteins; D020543:Proteome