title: Binding of soluble fibronectin to integrin alpha5 beta1 - link to focal adhesion redistribution and contractile shape.
abstract: Focal adhesions are randomly distributed across the ventral surface or along the edge of epithelial cells. In fibroblasts they orient centripetally and concentrate at a few peripheral sites connecting long F-actin stress fibers, causing a typical elongated, contractile morphology. Extensive remodeling of adhesions in fibroblasts also takes part in fibronectin fibrillogenesis, a process that depends on Rho-mediated contractility and results in the formation of a fibronectin matrix. Our current study shows that all these fibroblast characteristics are controlled by the ability of integrin alpha5 beta1 to bind soluble fibronectin molecules in their compact inactive conformation. The hypervariable region of the ligand-binding I-like domain of integrin alpha5 beta1 supports binding of soluble fibronectin. This supports the distribution of centripetally orientated focal adhesions in distinct peripheral sites, Rho activation and fibronectin fibrillogenesis through a mechanism that does not depend on Syndecan-4. Integrin alpha v beta3, even when locked in high affinity conformations for the RGD recognition motif shows no appreciable binding of soluble fibronectin and, consequently, fails to support the typical fibroblast focal adhesion distribution, Rho activity and fibronectin fibrillogenesis in the absence of integrin alpha5 beta1. The ability of alpha5 beta1 integrin to interact with soluble fibronectin may thus drive the cell-matrix adhesion and cytoskeletal organization required for a contractile, fibroblast-like morphology, perhaps explaining why alpha5 beta1 integrin, similarly to fibronectin, is essential for development.
authors.affiliation: Division of Toxicology, Leiden Amsterdam Center for Drug Research, Einsteinweg 55, Leiden University, Leiden CC, The Netherlands.
authors.forename: Stephan
authors.initials: S
authors.lastname: Huveneers
authors.forename: Hoa
authors.initials: H
authors.lastname: Truong
authors.forename: Reinhard
authors.initials: R
authors.lastname: Fässler
authors.forename: Arnoud
authors.initials: A
authors.lastname: Sonnenberg
authors.forename: Erik H J
authors.initials: EH
authors.lastname: Danen
mesh_terms: D000818:Animals; D001665:Binding Sites; D002460:Cell Line; D003599:Cytoskeleton / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D022001:Focal Adhesions / Q000378:metabolism*; D006801:Humans; D039081:Integrin alpha5beta1 / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008954:Models, Biological; D053671:Syndecan-4 / Q000378:metabolism; D014162:Transfection; D054460:rho-Associated Kinases / Q000378:metabolism
chemical_list: D005353:Fibronectins; D039081:Integrin alpha5beta1; D053671:Syndecan-4; D054460:rho-Associated Kinases