Interleukin-10-592C/A, -819C/T and -1082A/G promoter variants affect the susceptibility to nephropathy in Tunisian type 2 diabetes (T2DM) patients.
BACKGROUND
The Interleukin (IL)-10 polymorphic variants -1082G/A, -819C/T and -592C/A were linked with obesity, metabolic syndrome, and type 2 diabetes (T2DM). We investigated the hypothesis that IL-10 promoter polymorphisms may be associated with the progression of diabetic nephropathy (DN).


METHODS
Case-controlled study.


METHODS
Study subjects comprised of 515 DN patients, and 402 normoalbuminuric (DWN) T2DM patients.


METHODS
IL-10 genotyping was done by PCR-based assays, and the contributions of the IL-10 polymorphic variants to DN were analysed by haplotype analysis and multivariate regression analysis.


RESULTS
Decreased prevalence of (mutant) -819T allele and -819C/T genotype was seen in DN patients; neither the -1082G/A nor the -592C/A polymorphism was associated with DN. Three-loci haplotype (-1082GA/-819CT/-592CA) analysis identified GTC as DN-protective haplotype. Multivariate regression analysis confirmed the association of GTC haplotype (P = 0.045; OR = 0.56, 95% CI: 0.31-0.99), and in addition identified GTA haplotype (P = 0.044; OR = 0.54, 95% CI: 0.30-0.98) as independent predictors of DN after controlling for a number of covariates (age, sex, BMI; hypertension, glucose, HbA1c, DN duration, total cholesterol, medications).


CONCLUSIONS
This study suggests that IL-10 promoter polymorphism influence the risk of nephropathy in Tunisian T2DM patients.
Ezzidi
Intissar
I
Research unit of Biology and Genetics of Cancer and Haematological and Autoimmune diseases, Faculty of Pharmacy of Monastir, Monastir University, Monastir, Tunisia.
Mtiraoui
Nabil
N
Kacem
Maha
M
Mallat
Samir G
SG
Mohamed
Manel Ben Hadj
MB
Chaieb
Molka
M
Mahjoub
Touhami
T
Almawi
Wassim Y
WY
D000368:Aged; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D003924:Diabetes Mellitus, Type 2 / Q000208:ethnology / Q000235:genetics*; D003928:Diabetic Nephropathies / Q000208:ethnology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D016753:Interleukin-10 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D011110:Polymorphism, Genetic / Q000235:genetics*; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012307:Risk Factors; D014416:Tunisia
D016753:Interleukin-10