Diagnosis of multiple myeloma using enzyme immunoassay for detection of antibodies to N-acetyl glucosamine on microparticles.
Immunoassay on polymeric particles with covalently bound N-acetyl glucosamine was applied for detection of appropriate antibodies. Antibodies to N-acetyl glucosamine were detected in sera of patients with multiple myeloma. The assay was conducted on polymeric microparticles by enzyme immunoassay. The sensitivities of the assays on microparticles and on glass microtubes were compared. It was shown that the level of antibodies to N-acetyl glucosamine correlated with the number of myeloma cells. The comparison of assays on microparticles and glass tubes demonstrate the advantage of microparticle technology.
Melkonyan
Varduhi Z
VZ
Institute of Biochemistry National Academy of Sciences, Ministry of Health, Yerevan, Armenia.
Dagbashyan
Smbat S
SS
Melkikyan
Nata A
NA
Gasparyan
Vardan K
VK
D000117:Acetylglucosamine / Q000276:immunology*; D000906:Antibodies / Q000097:blood*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods* / Q000592:standards; D009101:Multiple Myeloma / Q000175:diagnosis*; D010316:Particle Size
D000906:Antibodies; D000117:Acetylglucosamine