title: Lactobacillus acidophilus 74-2 and butyrate induce cyclooxygenase (COX)-1 expression in gastric cancer cells.
abstract: Cyclo-oxygenase (COX) profile predicts prognosis of gastric cancer; COX-2 positive tumors are more often aggressive, and COX-2 suppression is protective against gastric cancer. In contrast, COX-1 suppression is harmful to the intestinal mucosa. The COX-1, COX-2, and COX-1ir expression profiles were measured with real-time PCR in primary (AGS) and metastatic (NCI-N87) gastric adenocarcinoma cell lines treated with butyrate, hyperosmolar medium, and, in the case of NCI-N87, cell-free supernatants of probiotic bacteria Lactobacillus acidophilus 74-2 and Bifidobacterium lactis 420. The cell lines showed differences in the profile when treated with either hyperosmolar medium or butyrate. In NCI-N87 COX-2 expression was higher but only COX-1 expression was significantly upregulated by butyrate. Similarly to butyrate, the cell-free supernatant of L. acidophilus 74-2 upregulated COX-1, while COX-2 expression remained unchanged. COX-1ir, including COX-3, was upregulated by probiotics and osmotic stress. In conclusion, consumption of L. acidophilus 74-2 could be beneficial for the expression of cytoprotective COX-1.
authors.affiliation: Department of Surgery, Helsinki University Central Hospital, Helsinki, Finland. pauli.puolakkainen@hus.fi
authors.forename: Anna
authors.initials: A
authors.lastname: Mahkonen
authors.forename: Heli
authors.initials: H
authors.lastname: Putaala
authors.forename: Harri
authors.initials: H
authors.lastname: Mustonen
authors.forename: Nina
authors.initials: N
authors.lastname: Rautonen
authors.forename: Pauli
authors.initials: P
authors.lastname: Puolakkainen
mesh_terms: D000230:Adenocarcinoma / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D001644:Bifidobacterium; D002087:Butyrates / Q000494:pharmacology*; D045744:Cell Line, Tumor; D051545:Cyclooxygenase 1 / Q000096:biosynthesis* / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000378:metabolism; D004790:Enzyme Induction; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D007779:Lactobacillus acidophilus; D009362:Neoplasm Metastasis; D009997:Osmotic Pressure; D016133:Polymerase Chain Reaction; D019936:Probiotics / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism; D012462:Saline Solution, Hypertonic; D013274:Stomach Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology; D013997:Time Factors
chemical_list: D002087:Butyrates; D012333:RNA, Messenger; D012462:Saline Solution, Hypertonic; D051545:Cyclooxygenase 1; D051546:Cyclooxygenase 2; C496511:PTGS1 protein, human; C496540:PTGS2 protein, human