Capillary electrophoresis-chemiluminescence determination of norfloxacin and prulifloxacin.
A capillary electrophoresis (CE)-chemiluminescence (CL) method for determining norfloxacin (NFLX) and prulifloxacin (PFLX) was developed based on the enhanced CL intensity of the cerium(IV)-sulfite-fluoroquinolone (FQ) reaction sensitized by terbium(III). The separation was conducted in buffer composed of 20 mM sodium citrate, 4 mM citric acid and 10 mM sodium sulfite at pH 6.1. The CL reagent solution consisted of 2 mM cerium(IV), 4 mM terbium(III) and 1.1 mM hydrochloric acid. NFLX and PFLX were baseline separated within 11 min with detection limits (S/N=3) of 0.057 and 0.084 microg mL(-1), respectively. The maximum intra- and inter-day relative standard deviations (R.S.D.s) of migration time of the analytes were less than 4.0% and 4.2%, respectively. The proposed method was applied to detect NFLX and PFLX in fortified urine sample and the results were comparable to high-performance liquid chromatography (HPLC)-UV method. Moreover, the high selectivity of the CL detection and the high-separation efficiency of CE render the method the potential of quick analyzing fluoroquinolones in real complex matrix.
Yang
Zhongju
Z
College of Chemistry, Beijing Normal University, Beijing 100875, PR China.
Wang
Xiaoli
X
Qin
Weidong
W
Zhao
Huichun
H
D002021:Buffers; D002563:Cerium / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D004148:Dioxolanes / Q000737:chemistry / Q000652:urine*; D019075:Electrophoresis, Capillary / Q000379:methods*; D024841:Fluoroquinolones / Q000737:chemistry / Q000652:urine*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008163:Luminescent Measurements / Q000379:methods*; D009643:Norfloxacin / Q000737:chemistry / Q000652:urine*; D010879:Piperazines / Q000737:chemistry / Q000652:urine*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013725:Terbium / Q000737:chemistry
D002021:Buffers; D004148:Dioxolanes; D024841:Fluoroquinolones; D010879:Piperazines; D013725:Terbium; D002563:Cerium; C074191:prulifloxacin; D009643:Norfloxacin