title: Chronic riluzole treatment increases glucose metabolism in rat prefrontal cortex and hippocampus.
abstract: Riluzole is believed to modulate glutamatergic function by reducing glutamate release and facilitating astroglial uptake. We measured (13)C labeling in metabolites in prefrontal cortex and hippocampus during a 10 mins infusion of [1-(13)C]glucose in urethane anesthetized rats treated with riluzole (21 days, 4 mg/kg per day, i.p.) or saline. Total and (13)C concentrations of metabolites were determined in extracts using (1)H-[(13)C] NMR spectroscopy. In prefrontal cortex (P<0.05) and hippocampus (P<0.05) riluzole increased (13)C labeling over saline in glutamate-C4 (to 112% and 130%), GABA-C2 (to 142% and 171%), and glutamine-C4 (to 118% and 233%) without affecting total metabolite levels (P>0.2). Our findings indicate that contrary to expectation chronic riluzole enhanced glucose oxidative metabolism and glutamate/glutamine cycling.
authors.affiliation: Department of Diagnostic Radiology, Magnetic Resonance Research Center, Yale University School of Medicine, New Haven, Connecticut, USA. golam.chowdhury@yale.edu
authors.forename: Golam M I
authors.initials: GM
authors.lastname: Chowdhury
authors.forename: Mounira
authors.initials: M
authors.lastname: Banasr
authors.forename: Robin A
authors.initials: RA
authors.lastname: de Graaf
authors.forename: Douglas L
authors.initials: DL
authors.lastname: Rothman
authors.forename: Kevin L
authors.initials: KL
authors.lastname: Behar
authors.forename: Gerard
authors.initials: G
authors.lastname: Sanacora
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D001786:Blood Glucose / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018696:Neuroprotective Agents / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology; D017397:Prefrontal Cortex / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D019782:Riluzole / Q000008:administration & dosage / Q000493:pharmacokinetics / Q000494:pharmacology; D013997:Time Factors
chemical_list: D000596:Amino Acids; D001786:Blood Glucose; D018696:Neuroprotective Agents; D019782:Riluzole; D005947:Glucose