H2B ubiquitylation plays a role in nucleosome dynamics during transcription elongation.
The monoubiquitylation of histone H2B has been associated with transcription initiation and elongation, but its role in these processes is poorly understood. We report that H2B ubiquitylation is required for efficient reassembly of nucleosomes during RNA polymerase II (Pol II)-mediated transcription elongation in yeast. This role is carried out in cooperation with the histone chaperone Spt16, and in the absence of H2B ubiquitylation and functional Spt16, chromatin structure is not properly restored in the wake of elongating Pol II. Moreover, H2B ubiquitylation and Spt16 play a role in each other's regulation. H2B ubiquitylation is required for the stable accumulation of Spt16 at the GAL1 coding region, and Spt16 regulates the formation of ubiquitylated H2B both globally and at the GAL1 gene. These data provide a mechanism linking H2B ubiquitylation to Spt16 in the regulation of nucleosome dynamics during transcription elongation.
Fleming
Alastair B
AB
Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, USA.
Kao
Cheng-Fu
CF
Hillyer
Cory
C
Pikaart
Michael
M
Osley
Mary Ann
MA
D018797:Cell Cycle Proteins / Q000378:metabolism; D002843:Chromatin / Q000378:metabolism; D005686:Galactokinase / Q000378:metabolism; D006657:Histones / Q000378:metabolism*; D007700:Kinetics; D008239:Lysine / Q000378:metabolism; D008745:Methylation; D009707:Nucleosomes / Q000378:metabolism*; D011485:Protein Binding; D012319:RNA Polymerase II / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism; D014157:Transcription Factors / Q000378:metabolism; D014158:Transcription, Genetic; D035602:Transcriptional Elongation Factors; D054875:Ubiquitination
D018797:Cell Cycle Proteins; D002843:Chromatin; D006657:Histones; D009707:Nucleosomes; C071238:SPT16 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase; D012319:RNA Polymerase II; D008239:Lysine