Vaccination with clumping factor A and fibronectin binding protein A to prevent Staphylococcus aureus infection of an aortic patch in mice.
Staphylococcus aureus is a leading cause of ventricular assist device-related infections. This study evaluated the protective effect against S. aureus infection of active and passive immunization that targeted 3 proteins involved in bacterial attachment to a murine intra-aortic polyurethane patch. Active immunization of mice with a combination of the A domains of clumping factor A (ClfA), fibronectin-binding protein A (FnBPA) and fibronectin-binding protein B or passive immunization with monoclonal antibodies against ClfA and FnBPA resulted in a higher level of protection than that obtained by vaccination with either protein or antibody alone. The combination of antibodies or protein antigens appears to provide enhanced protection against prosthetic-device infection.
Arrecubieta
Carlos
C
Divisions of Infectious Diseases, Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
Matsunaga
Iwao
I
Asai
Tomohiro
T
Naka
Yoshifumi
Y
Deng
Mario C
MC
Lowy
Franklin D
FD
D018829:Adhesins, Bacterial / Q000008:administration & dosage* / Q000276:immunology; D000818:Animals; D001011:Aorta; D001428:Bacterial Vaccines / Q000008:administration & dosage / Q000737:chemistry; D003030:Coagulase / Q000008:administration & dosage* / Q000276:immunology; D004195:Disease Models, Animal; D004697:Endocarditis, Bacterial / Q000378:metabolism / Q000382:microbiology* / Q000473:pathology; D007116:Immunization, Passive; D051379:Mice; D013203:Staphylococcal Infections / Q000276:immunology / Q000517:prevention & control*; D013211:Staphylococcus aureus; D014611:Vaccination; D014774:Virulence
D018829:Adhesins, Bacterial; D001428:Bacterial Vaccines; D003030:Coagulase; C078635:fibronectin-binding proteins, bacterial