The fluorescence enhancement of the protein adsorbed on the surface of Ag nanoparticle.
The fluorescence enhancement of the BSA adsorbed on the surface of Ag nanoparticles is reported, where non-fluorescent collagen is used as the separator between the BSA and Ag nanoparticles. The study indicates that Ag nanoparticles can enhance the fluorescence of the BSA, especially the fluorescence of the tyrosine residues with lower quantum. Three types of Ag nanoparticles are evaluated including Ag island film, Ag colloids and fractal Ag electrode. Of them Ag island film is the best. The investigation suggests that the fluorescence enhancement of the BSA is related to the adsorption of the BSA on the surface of Ag island film through the hydrophobic interaction, while the collagen can promote the adsorption of the BSA on the surface of Ag island film and change its conformation, resulting in the interaction between BSA and Ag island film.
Sun
Changxia
C
Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, People's Republic of China.
Wu
Xia
X
Ding
Honghong
H
Zhao
Liangliang
L
Wang
Fei
F
Yang
Jinghe
J
Liu
Xiaoyu
X
D000327:Adsorption; D005453:Fluorescence; D008567:Membranes, Artificial; D053768:Metal Nanoparticles / Q000737:chemistry*; D011791:Quartz / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
D008567:Membranes, Artificial; D011791:Quartz; D012710:Serum Albumin, Bovine; D012834:Silver