title: Acinetobacter sp. strain Ths, a novel psychrotolerant and alkalitolerant bacterium that utilizes hydrocarbon.
abstract: A novel psychrotolerant, alkalitolerant bacterium, strain Ths, was isolated from a soil sample immersed in hot spring water containing hydrocarbons and grown on a chemically defined medium containing n-tetradecane as the sole carbon source. The isolate grew at 0 degrees C but not at temperatures higher than 45 degrees C; its optimum growth temperature was 27 degrees C. It grew in the pH range of 7-9. The strain utilized C(13)-C(30) n-alkane and fluorene at pH 9 and 4 degrees C. To our knowledge, this is the first report on the bacterium that utilizes a wide range of hydrocarbons at a high pH and a low temperature. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Ths is closely related to genomic species 6 ATCC 17979 (99.1% similarity), genomic species BJ13/TU14 ATCC 17905 (97.8% similarity), genomic species 9 ATCC 9957 (97.6% similarity) belonging to the genus Acinetobacter and to Acinetobacter calcoaceticus JCM 6842(T) (97.5% similarity). DNA-DNA hybridization revealed that the isolate has 62, 25, 18 and 19% relatedness, respectively, to genomic species 6 ATCC 17979, genomic species BJ13/TU14 ATCC 17905, genomic species 9 ATCC 9957 and A. calcoaceticus, respectively.
authors.affiliation: Research Institute of Genome-Based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Tsukisamu-Higashi, Toyohira-ku, Sapporo 062-8517, Japan.
authors.forename: Keiko
authors.initials: K
authors.lastname: Yamahira
authors.forename: Kikue
authors.initials: K
authors.lastname: Hirota
authors.forename: Kenji
authors.initials: K
authors.lastname: Nakajima
authors.forename: Naoki
authors.initials: N
authors.lastname: Morita
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Nodasaka
authors.forename: Isao
authors.initials: I
authors.lastname: Yumoto
mesh_terms: D000150:Acinetobacter / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000235:genetics; D006838:Hydrocarbons / Q000378:metabolism*; D007700:Kinetics; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012988:Soil Microbiology; D013379:Substrate Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D006838:Hydrocarbons; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S