Archvillin anchors in the Z-line of skeletal muscle via the nebulin C-terminus.
Z-Line of skeletal muscle is a complex protein network that likely plays an important role in signaling and muscle homeostasis. We used the yeast two-hybrid system to search for potential novel ligands of the Z-line portion of nebulin. We found that the C-terminal region of nebulin (residues 6457-6528) interacted with the C-terminus of archvillin (residues 1419-1687). Archvillin is a membrane skeletal protein that localizes to costameres, specialized adhesion sites in muscle. The binding sites between nebulin and archvillin were characterized using the yeast two-hybrid system, in vitro pull-down assays, and colocalization experiments in COS-7 cells. Our data suggest a model in which archvillin attaches directly to the Z-line through an interaction with the nebulin C-terminus. The interaction between nebulin and archvillin may provide a direct link between the sarcolemma and myofibrillar Z-lines.
Lee
Min-A
MA
Department of Biology, Inje University, Gimhae 621-749, Republic of Korea.
Joo
Young Mi
YM
Lee
Yeong Mi
YM
Kim
Hyun Suk
HS
Kim
Ji-Hee
JH
Choi
Jae-Kyong
JK
Ahn
Seung-Ju
SJ
Min
Byung-In
BI
Kim
Chong-Rak
CR
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012508:Sarcolemma / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
D008565:Membrane Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; C109413:SVIL protein, human; C035620:nebulin