Photometric microplate assay for estimation of the efficacy of paraoxon-inhibited acetylcholinesterase reactivation.
Photometric microplate assay was performed for testing of paraoxon-inhibited acetylcholinesterase (AChE) using three reactivators for reactivation purposes: obidoxime, pralidoxime, and HI-6. 3-D graphs (percent of reactivation vs. concentration of reactivator and vs. time of reactivator effecting) were constructed for each reactivator to compare their efficacy. The best results were obtained using obidoxime where reactivation was near to 80%. Suitability of photometric microplates for following of reactivation procedures is discussed.
Pohanka
Miroslav
M
Centre of Biological Defence, Central Military Institute of Health, Techonin, Czech Republic.
Jun
Daniel
D
Kuca
Kamil
K
D000110:Acetylcholinesterase / Q000378:metabolism*; D002800:Cholinesterase Inhibitors / Q000737:chemistry* / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D010261:Paraoxon / Q000737:chemistry* / Q000494:pharmacology*; D010783:Photometry
D002800:Cholinesterase Inhibitors; D000110:Acetylcholinesterase; D010261:Paraoxon