title: Use of cyclohexanedimethanol as a nonflammable organic solvent for industrial scale reversed phase chromatography.
abstract: This work demonstrates that cyclohexanedimethanol is an effective nonflammable organic solvent for the pilot scale reversed phase chromatography of recombinant apolipoprotein A-I(Milano). Cyclohexanedimethanol has low viscosity in water, enabling the use of conventional low pressure process scale chromatography columns and hardware. Results from pilot scale manufacturing using a 30 cm diameter CG71M packed column indicate that a 5.7 log reduction in E. coli host cell protein with >80% yield can be obtained for the purification of apolipoprotein A-I(Milano).
authors.affiliation: Pfizer Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA. alan.k.hunter@pfizer.com
authors.forename: Alan K
authors.initials: AK
authors.lastname: Hunter
authors.forename: Sydney D
authors.initials: SD
authors.lastname: Hoeltzli
authors.forename: Gary V
authors.initials: GV
authors.lastname: Johnson
authors.forename: Mark E
authors.initials: ME
authors.lastname: Gustafson
authors.forename: Sa V
authors.initials: SV
authors.lastname: Ho
mesh_terms: D016632:Apolipoprotein A-I / Q000032:analysis / Q000235:genetics; D002853:Chromatography, Liquid / Q000379:methods*; D003510:Cyclohexanes / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D009930:Organic Chemicals / Q000737:chemistry*; D011994:Recombinant Proteins / Q000032:analysis; D012997:Solvents / Q000737:chemistry*
chemical_list: D016632:Apolipoprotein A-I; D003510:Cyclohexanes; D009930:Organic Chemicals; D011994:Recombinant Proteins; D012997:Solvents; C023353:1,4-cyclohexanedimethanol