Separation of inorganic anions on a high capacity porous polymeric monolithic column and application to direct determination of anions in seawater.
A commercially available 4.6 mm id x 50 mm polymethacrylate-based monolithic strong anion exchange column (ProSwift SAX-1S) designed for the separation of proteins has been successfully used to separate small inorganic anions in the presence of a seawater sample matrix. Using a hydroxide eluent with suppressed conductivity detection the ion exchange capacity of this column declined over time; however, using KCl as the eluent, the column performance was stable with a capacity of 530 microequiv. for nitrate. The optimum conditions for the separation of iodate, bromate, nitrite, bromide and nitrate were assessed by constructing van Deemter plots using 1.00 and 0.100 M KCl. Efficiencies of up to 26 700 plates/m were recorded using 1.00 M KCl, at a flow rate of 0.20 mL/min but iodate was not baseline resolved from the void peak. By reducing the concentration of the eluent to 0.100 M, efficiencies of up to 39 900 plates/m could be obtained at 0.35 mL/min. By employing a linear gradient ranging from 0.05 to 1.00 M KCl the ions dissolved in distilled water or a salt water matrix could be baseline separated in less than 3 min at a flow rate of 2.50 mL/min.
Evenhuis
Christopher J
CJ
Australian Centre for Research on Separation Science, School of Chemistry, University of Tasmania, Tasmania, Australia.
Buchberger
Wolfgang
W
Hilder
Emily F
EF
Flook
Kelly J
KJ
Pohl
Christopher A
CA
Nesterenko
Pavel N
PN
Haddad
Paul R
PR
D000838:Anions / Q000032:analysis*; D001959:Bromates / Q000032:analysis; D001965:Bromides / Q000032:analysis; D002712:Chlorides / Q000032:analysis; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods; D005459:Fluorides / Q000032:analysis; D007452:Iodates / Q000032:analysis; D009573:Nitrites / Q000032:analysis; D010316:Particle Size; D011109:Polymethacrylic Acids / Q000737:chemistry*; D016062:Porosity; D012623:Seawater / Q000737:chemistry*; D013499:Surface Properties; D013997:Time Factors
D000838:Anions; D001959:Bromates; D001965:Bromides; D002712:Chlorides; D007452:Iodates; D009573:Nitrites; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D005459:Fluorides