Specific N-terminal protein labelling: use of FMDV 3C pro protease and native chemical ligation.
We report an effective strategy for generating N-terminal cysteinyl proteins by proteolytic cleavage using the enzyme 3C pro, suitable for a wide range of applications via native chemical ligation.
Busch
Gillian K
GK
Department of Chemistry and the Chemical Biology Centre, Imperial College London, South Kensington Campus, London, UK.
Tate
Edward W
EW
Gaffney
Piers R J
PR
Rosivatz
Erika
E
Woscholski
Rudiger
R
Leatherbarrow
Robin J
RJ
D000086042:3C Viral Proteases; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry*; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D029544:Foot-and-Mouth Disease Virus / Q000201:enzymology; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins / Q000378:metabolism*
D011506:Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases; D003545:Cysteine