title: High osmolality induces the kidney-specific chloride channel CLC-K1 by a serum and glucocorticoid-inducible kinase 1 MAPK pathway.
abstract: The kidney-specific chloride channels CLC-K1/2 and their functionally important subunit barttin, by mediating solute transport in medulla, contribute to the osmotic gradient. We sought to determine whether they themselves are regulated by variations of osmolality. The expression of CLC-K1 and barttin mRNA and protein was significantly increased in a distal convoluted tubule cell line after a shift to high osmolar medium. This upregulation paralleled that of serum and glucocorticoid-inducible kinase 1 (SGK1), a gene known to be upregulated by cell shrinkage. Specific knockdown of SGK1 or addition of the p38 MAPK pathway inhibitor SB203580 abolished the induction of SGK1, CLC-K1 and barttin by high osmolarity suggesting that a functional MAPK pathway is required to mediate osmotic-driven induction of all three genes. The physiological relevance of our in vitro data was confirmed by water deprivation of male C57BL6 mice, which caused a significant increase in serum osmolality along with induction of CLC-K1, barttin and SGK1. Our study shows that change in intracellular volume, because of high osmolality, result in SGK1 upregulation and the subsequent increase of CLC-K1/barttin expression in distal renal tubular cells in vivo and in vitro.
authors.affiliation: Department of Internal Medicine II, University of Regensburg, Regensburg, Germany. tobias.bergler@klinik.uni-regensburg.de
authors.forename: Tobias
authors.initials: T
authors.lastname: Bergler
authors.forename: Benjamin
authors.initials: B
authors.lastname: Stoelcker
authors.forename: Roland
authors.initials: R
authors.lastname: Jeblick
authors.forename: Stephan W
authors.initials: SW
authors.lastname: Reinhold
authors.forename: Konrad
authors.initials: K
authors.lastname: Wolf
authors.forename: Günter A J
authors.initials: GA
authors.lastname: Riegger
authors.forename: Bernhard K
authors.initials: BK
authors.lastname: Krämer
mesh_terms: D000818:Animals; D002460:Cell Line; D018118:Chloride Channels / Q000235:genetics*; D005786:Gene Expression Regulation / Q000502:physiology*; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism*; D007684:Kidney Tubules / Q000166:cytology / Q000378:metabolism*; D020935:MAP Kinase Signaling System; D008297:Male; D008565:Membrane Proteins / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D009994:Osmolar Concentration; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis; D015854:Up-Regulation / Q000235:genetics; D014882:Water-Electrolyte Balance; D000099184:Serum-Glucocorticoid Regulated Kinases
chemical_list: D018118:Chloride Channels; D017874:Immediate-Early Proteins; D008565:Membrane Proteins; D017346:Protein Serine-Threonine Kinases; D012333:RNA, Messenger; D000099184:Serum-Glucocorticoid Regulated Kinases; C486687:Bsnd protein, mouse; C489933:Clcnka protein, mouse