title: The electrophysiological properties of the mucosal barrier in the injured mouse jejunum in Ussing chambers.
abstract: We previously suggested in the in vitro mouse jejunum that when the primary epithelial barrier on the villi was largely disabled, a secondary mucosal barrier substitutes for the primary one. This study showed that the mucosal Ca(2+) removal did not further deteriorate the histology but increased transmural conductance and lucifer yellow flux, and decreased forskolin-induced potential difference. It is suggested that in this injury model the postulated secondary barrier is Ca(2+)-dependent and likely to be present on or immediately beneath the mucosal surface.
authors.affiliation: Department of Nutritional Sciences, School of Nutritional Sciences, Nagoya University of Arts and Sciences, Nisshin, Aichi, Japan. e-ina@nuas.ac.jp
authors.forename: Eiko
authors.initials: E
authors.lastname: Inagaki-Tachibana
authors.forename: Hisayoshi
authors.initials: H
authors.lastname: Hayashi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Suzuki
mesh_terms: D000818:Animals; D001693:Biological Transport, Active; D002122:Calcium Chloride / Q000378:metabolism; D002463:Cell Membrane Permeability; D005576:Colforsin / Q000494:pharmacology; D004058:Diffusion; D015194:Diffusion Chambers, Culture; D004553:Electric Conductivity; D004594:Electrophysiology; D004847:Epithelial Cells / Q000378:metabolism; D007413:Intestinal Mucosa / Q000293:injuries* / Q000503:physiopathology*; D007583:Jejunum / Q000293:injuries* / Q000503:physiopathology*; D008297:Male; D008564:Membrane Potentials; D051379:Mice; D010539:Permeability; D013997:Time Factors
chemical_list: D005576:Colforsin; D002122:Calcium Chloride