Immunochemical studies of foot-and-mouth disease. III. Acridine orange staining of agar gel precipitin reactions.
Agar gel diffusion precipitin reactions obtained with foot-and-mouth disease virus antigens were examined by an acridine orange staining procedure. After treatment with 0.01% acridine orange, precipitin bands produced by the virus particles (140 S antigen) fluoresced flame red when examined under ultraviolet light and with 0.001% to 0.0001% acridine orange, a yellow green. Treatment with RNase prior to staining inhibited the reaction, whereas, treatment with DNase did not. This was in accord with the known RNA nature of foot-and-mouth disease virus. Precipitin bands formed by the 75 S, 12 S, and infection-associated (VIA) antigenic components did not take up acridine orange, an indication that they do not contain detectable amounts of nucleic acid.
Cowan
K M
KM
Plum Island Animal Disease Laboratory, Animal Disease and Parasite Research Division, Agricultural Research Service, U.S. Department of Agriculture, Greenport, New York 11944, USA.
Graves
J H
JH