[Influence of microwave radiation on synapsin I expression in PC12 cells and its mechanism].
OBJECTIVE
To investigate the effect of microwave radiation on expression and phosphorylation of synapsin I and to discover the mechanism by research on the change of expression of BDNF and its receptor, TrkB.


METHODS
PC12 cells were exposed to microwave with average power density being 30 mW/cm(2). HPLC was used to detect the release of amino acids; RT-PCR, Western blot and immunocytochemistry were used to detect the expressions of synapsin I, BDNF and TrkB; immune co-precipitation was used to study the interaction of BDNF and TrkB.


RESULTS
It resulted in the decrease of the release of Asp, Glu, GABA and Gly at 1 h (P<0.01) after radiation. Protein of synapsin I was decreased in 9 h-2 d (P<0.01 or P<0.05); its mRNA was decreased in 3-9 h and increased at 1 d (P<0.01 or P<0.05); its phosphorylation was decreased at 3 h, increased at 1 d, and decreased at 2 d again (P<0.01 or P<0.05) after radiation. Protein of BDNF was decreased at 3 h and increased in 1-2 d (P<0.01 or P<0.05); its mRNA were decreased in 3-9 h, increased at 1d, and decreased at 2 d again (P<0.01 or P<0.05) after radiation. Protein of TrkB was increased in 3 h-1 d (P<0.01 or P<0.05); its mRNA decreased at 3 h and 2 d (P<0.01) after radiation. The interaction between BDNF and TrkB was increased in 3-9 h, but decreased in 1-2 d (P<0.01 or P<0.05) after radiation.


CONCLUSIONS
Microwave radiation can induce the decrease of the release of amino acids and the expression and phosphorylation of synapsin I, and the abnormality of expressions and interaction of BDNF and TrkB in PC12 cells. The factors might play a role in the injury and repair of information transmission in PC12 cells.
Wang
Li-Feng
LF
Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
Peng
Rui-Yun
RY
Hu
Xiang-Jun
XJ
Gao
Ya-Bing
YB
Wang
Shui-Ming
SM
Gao
Rong-Lian
RL
Xu
Xin-Ping
XP
Su
Zhen-Tao
ZT
Dong
Ji
J
D000818:Animals; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008872:Microwaves; D016716:PC12 Cells; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D051381:Rats; D020813:Receptor, trkB / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016704:Synapsins / Q000235:genetics / Q000378:metabolism*
D019208:Brain-Derived Neurotrophic Factor; D016704:Synapsins; D020813:Receptor, trkB