Combination treatment of human umbilical cord matrix stem cell-based interferon-beta gene therapy and 5-fluorouracil significantly reduces growth of metastatic human breast cancer in SCID mouse lungs.
Umbilical cord matrix stem (UCMS) cells that were engineered to express interferon-beta (IFN-beta) were transplanted weekly for three weeks into MDA 231 breast cancer xenografts bearing SCID mice in combination with 5-fluorouracil (5-FU). The UCMS cells were found within lung tumors but not in other tissues. Although both treatments significantly reduced MDA 231 tumor area in the SCID mouse lungs, the combined treatment resulted in a greater reduction in tumor area than by either treatment used alone. These results indicate that a combination treatment of UCMS-IFN-beta cells and 5-FU is a potentially effective therapeutic procedure for breast cancer.
Rachakatla
Raja Shekar
RS
Department of Anatomy & Physiology, Kansas State University, Manhattan, Kansas 66506, USA.
Pyle
Marla M
MM
Ayuzawa
Rie
R
Edwards
Sarah M
SM
Marini
Frank C
FC
Weiss
Mark L
ML
Tamura
Masaaki
M
Troyer
Deryl
D
D000818:Animals; D000964:Antimetabolites, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D017024:Chemotherapy, Adjuvant; D036101:Cord Blood Stem Cell Transplantation; D017077:Culture Media, Conditioned / Q000378:metabolism; D005260:Female; D053686:Fetal Stem Cells / Q000378:metabolism*; D005472:Fluorouracil / Q000494:pharmacology*; D015316:Genetic Therapy / Q000379:methods*; D006801:Humans; D016899:Interferon-beta / Q000235:genetics / Q000378:metabolism*; D008175:Lung Neoplasms / Q000378:metabolism / Q000556:secondary / Q000601:surgery / Q000628:therapy*; D051379:Mice; D016513:Mice, SCID; D013997:Time Factors; D014161:Transduction, Genetic; D023041:Xenograft Model Antitumor Assays
D000964:Antimetabolites, Antineoplastic; D017077:Culture Media, Conditioned; D016899:Interferon-beta; D005472:Fluorouracil