title: Microstructure and glycosaminoglycan ratio of canine cornea after reconstructive transplantation with glycerin-preserved porcine amniotic membranes.
abstract: OBJECTIVE
  Although amniotic membranes of canine, feline, and equine species have some advantages as corneal transplantation material in many canine ocular diseases, their softness, thinness, and low availability can pose problems. As an alternative, the more abundant porcine amniotic membranes may be used. This paper describes the use of glycerin-preserved porcine amniotic membranes in corneal transplantation in eight normal dogs.
  
  
  METHODS
  A 0.4-mm deep recipient bed in the axial cornea of the OS of all dogs was created using an 8-mm Barron radial vacuum trephine. The recipient bed was then filled with amnion, and the entire cornea was covered with another piece of the glycerin-preserved membrane. The ocular signs evaluated were corneal opacity and corneal vascularization. The dogs were euthanized on days 5, 10, 20, or 40 after surgery, and samples were collected to evaluate corneal thickness, parenchymal cell number, mean collagen fibril diameter, collagen fibril content and the glycosaminoglycan (GAG) ratio.
  
  
  RESULTS
  Corneal opacity was observed immediately after surgery. Restoration of corneal transparency, regression of corneal vascularization, and visualization of the pupil and iris were noted on day 40.
  
  
  CONCLUSIONS
  The clinical observations were supported histologically by regained corneal thickness, parenchymal cell number, mean collagen fibril diameter, collagen fibril content, and GAG ratio, suggesting that this technique may be a novel method for the treatment of ocular surface disorders.
authors.affiliation: Department of Small Animal Clinical Science, School of Veterinary Medicine, Rakuno Gakuen University, 582-1 Bunkyodai - Midorimachi, Ebetsu, Hokkaido 069-8501, Japan. tsuzuki@rakuno.ac.jp
authors.forename: Keiko
authors.initials: K
authors.lastname: Tsuzuki
authors.forename: Kazuto
authors.initials: K
authors.lastname: Yamashita
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Izumisawa
authors.forename: Tadao
authors.initials: T
authors.lastname: Kotani
mesh_terms: D000650:Amnion / Q000637:transplantation*; D000818:Animals; D003315:Cornea / Q000473:pathology / Q000648:ultrastructure; D003316:Corneal Diseases / Q000473:pathology / Q000601:surgery / Q000662:veterinary*; D016039:Corneal Transplantation / Q000379:methods / Q000662:veterinary*; D004283:Dog Diseases / Q000601:surgery*; D004285:Dogs; D005260:Female; D006025:Glycosaminoglycans / Q000032:analysis; D008297:Male; D046529:Microscopy, Electron, Transmission / Q000662:veterinary; D011897:Random Allocation; D013552:Swine; D014183:Transplantation, Heterologous / Q000662:veterinary; D016896:Treatment Outcome
chemical_list: D006025:Glycosaminoglycans