title: Kinetic model of disaccharide oxidation by Agrobacterium tumefaciens.
abstract: Disaccharides were microbially transformed to their corresponding 3-keto-derivatives by resting cells of Agrobacterium tumefaciens NCPPB 396. The kinetics and yield of this highly specific oxidation depend on several factors. The oxygen concentration especially has a major influence on the production of 3-keto-derivatives and was investigated kinetically with respect to low stationary oxygen concentrations in solution. Experiments showed unconventional results that conflicted with normal Michaelis-Menten kinetics. A kinetic model was developed and the kinetic constants were calculated. The model and experimental data for sucrose, maltose, iso-maltulose (palatinose), and leucrose are in good agreement with each other. Initial reaction rates with different sugars using constant oxygen concentrations resulted in a Michaelis-Mentent type function. The complete kinetics, including the effect of disaccharide and oxygen concentrations, are presented.
authors.affiliation: Sugar Institute, Institut für Technologie der Kohlenhydrate, Technical University of Braunschweig, Langer Kamp 5, D-38106 Braunschweig, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Walter
authors.forename: H J
authors.initials: HJ
authors.lastname: Jördening