title: Characterization of subcellular localization and Ca2+ modulation of calsenilin/DREAM/KChIP3.
abstract: Earlier reports found that calsenilin is a transcriptional repressor or a subunit of plasma membrane channel, and indicated that calsenilin was present in the nucleus or plasma membrane. Immunohistochemical and subcellular fractionation analysis, however, revealed that calsenilin/DREAM/KChIP3 was distributed throughout the cytoplasm of SK-N-BE2(C), Jurkat, and HeLa cells. In addition, the expression of calsenilin suppressed the ATP-induced increase in intracellular Ca2+ concentrations. By increase in intracellular calcium concentration, calsenilin was translocated into the nucleus.
authors.affiliation: College of Pharmacy, Sungkyunkwan University, Jangan-gu, Suwon, Korea.
authors.forename: Ha-Na
authors.initials: HN
authors.lastname: Woo
authors.forename: Jae-Woong
authors.initials: JW
authors.lastname: Chang
authors.forename: Yun-Hyung
authors.initials: YH
authors.lastname: Choi
authors.forename: A-Ryeong
authors.initials: AR
authors.lastname: Gwon
authors.forename: Yong-Keun
authors.initials: YK
authors.lastname: Jung
authors.forename: Dong-Gyu
authors.initials: DG
authors.lastname: Jo
mesh_terms: D000818:Animals; D019556:COS Cells; D002118:Calcium / Q000032:analysis / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism*; D002522:Chlorocebus aethiops; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D042541:Intracellular Space / Q000378:metabolism; D019169:Jurkat Cells; D051605:Kv Channel-Interacting Proteins / Q000235:genetics / Q000378:metabolism*; D008856:Microscopy, Fluorescence; D010957:Plasmids / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: C496749:KCNIP3 protein, human; D051605:Kv Channel-Interacting Proteins; D011993:Recombinant Fusion Proteins; D012097:Repressor Proteins; D049452:Green Fluorescent Proteins; D002118:Calcium