Delivery of immunotherapy with peptide-pulsed blood in macaques.
Simple and effective delivery methods for cellular immunotherapies are needed. We assessed ex vivo pulsing of overlapping SIV Gag 15mer peptides onto either whole blood or PBMC in 15 randomly assigned SIV-infected macaques. Both delivery methods were safe and immunogenic, stimulating high levels of broad and polyfunctional Gag-specific CD4 and CD8 T cells. Delivery of overlapping Gag peptides via either whole blood or PBMC is suitable for clinical evaluation.
De Rose
Robert
R
Department of Microbiology and Immunology, University of Melbourne, 3010, Australia.
Fernandez
Caroline S
CS
Loh
Liyen
L
Peut
Viv
V
Mason
Rosemarie D
RD
Alcantara
Sheilajen
S
Reece
Jeanette
J
Kent
Stephen J
SJ
D000818:Animals; D001769:Blood / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015683:Gene Products, gag / Q000627:therapeutic use; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008254:Macaca nemestrina; D009842:Oligopeptides / Q000627:therapeutic use*; D011897:Random Allocation; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000628:therapy*
C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D009842:Oligopeptides; D007371:Interferon-gamma