Polymorphism in the ITS region of ribosomal DNA of Cochliobolus sativus isolates differing in xylanase production.
The restriction of PCR-amplified internal transcribed spacers (ITS) ofribosomal DNA was used to confirm the genetic variation among 22 isolates of Cochliobolus sativus differing in their xylanase production. Results show a high level of diversity of ITS-RFLP markers among the isolates. The molecular parameter used showed that C. sativus isolates reside in three phylogenetic groups. There was observed the resolution between clustering of isolates and their xylanase production level.
Bakri
Yasser
Y
Department of Molecular Biology and Biotechnology, AECS, Damascus, Syria.
Jawhar
Mohammed
M
Arabi
Mohammed Imad Eddin
MI
D001203:Ascomycota / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D014644:Genetic Variation; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013593:Syria
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D043364:Endo-1,4-beta Xylanases