Metabolic fingerprinting of Schistosoma mansoni infection in mice urine with capillary electrophoresis.
Schistosoma mansoni infection in mice has been fingerprinted using CE to study the capabilities of this technique as a diagnostic tool for this parasitic disease. Two modes of separation were used in generating the electrophoretic data, with each untreated urine sample the following methods were applied: (i) a fused-silica capillary, operating with an applied potential of 18 kV, in micellar EKC (MEKC) and (ii) a polyacrylamide-coated capillary, operating with an applied potential of -20 kV under zonal CZE conditions. By combining normal and reverse polarities in the data treatment we have extracted more information from the samples, which is a better approach for CE metabolomics. The traditional problems associated with variability in electrophoretic peak migration times for analytes were countered by using a dynamic programming algorithm for the electropherograms alignment. Principal component analyses of these aligned electropherograms and partial least square discriminant analysis (PLS-DA) data are shown to provide a valuable means of rapid and sample classification. This approach may become an important tool for the identification of biomarkers, diagnosis and disease surveillance.
García-Pérez
Isabel
I
Facultad de Farmacia, Universidad San Pablo-CEU, Campus Montepríncipe, Boadilla del Monte, Madrid, Spain.
Whitfield
Philip
P
Bartlett
Ann
A
Angulo
Santiago
S
Legido-Quigley
Cristina
C
Hanna-Brown
Melissa
M
Barbas
Coral
C
D000180:Acrylic Resins; D000818:Animals; D015415:Biomarkers; D019075:Electrophoresis, Capillary / Q000379:methods*; D005260:Female; D016018:Least-Squares Analysis; D055432:Metabolomics; D051379:Mice; D008823:Micelles; D010865:Pilot Projects; D025341:Principal Component Analysis; D012550:Schistosoma mansoni / Q000302:isolation & purification* / Q000378:metabolism; D012555:Schistosomiasis mansoni / Q000175:diagnosis* / Q000469:parasitology / Q000652:urine; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide; D013997:Time Factors; D016482:Urinalysis / Q000379:methods*; D000099166:Polyacrylamides
D000180:Acrylic Resins; D015415:Biomarkers; D008823:Micelles; D012822:Silicon Dioxide; D000099166:Polyacrylamides