title: Production of actinorhodin by Streptomyces coelicolor A3(2) grown in chemostat culture.
abstract: Streptomyces coelicolor was grown in variously limited chemostat cultures and the specific rate of extracellular actinorhodin production (q(actinorhodin)) was measured. The highest q(actinorhodin) values were observed in glucose- or ammonia-limited cultures, whereas almost no actinorhodin was produced in sulfate-, phosphate-, potassium-, or magnesium-limited cultures. The effect of the dilution rate on actinorhodin production was studied in glucose-limited cultures. It was found that q(actinorhodin) was highest at D = 0.06h(-1), which was well below the maximal D value tested (0.14 h(-1)). This explains why, in batch cultures, actinorhodin production starts at the onset of the stationary phase. It was also found that the use of nitrilotriacetate instead of citrate as a chelating agent had a negative effect on actinorhodin production. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 577-582, 1997.
authors.affiliation: Department of Fermentation Chemistry and Bioengineering, Institute of Chemical Technology, Technická 5, 16628 Prague 6, Czech Republic.
authors.forename: K
authors.initials: K
authors.lastname: Melzoch
authors.forename: M J
authors.initials: MJ
authors.lastname: de Mattos
authors.forename: O M
authors.initials: OM
authors.lastname: Neijssel