title: [Overexpression of the truncated fosb isoform (Delta2Deltafosb) increases nodus in CD1 primary osteoblastes].
abstract: The recombinant adenoviruses which can overexpress fosb and Delta2Deltafosb proteins were produced. Primary calvarial osteoblasts from new born CD1 mice were infected with those adenoviruses by different proportion of Ad-Delta2Deltafosb and induced by 5 mmol/L beta-glycerophosphate, 50 microg/ml ascorbic acid and 10 nmol/L dexamethasone for 23 days and 29 days, respectively. The results by Alizarin Red Staining showed that the primary osteoblasts overexpressing Delta2Deltafosb protein formed more nodus than the control cells and the nodus in overexpressing Delta2Deltafosb protein osteoblasts were smaller than those in control cells on day 23. Differentiation of the primary osteoblasts overexpressing Delta2Deltafosb protein increased more than that of the control cells on day 29. It is an evidence for the reason that bone density of Delta2Deltafosb transgenic mice was markedly increased throughout the skeleton in new born and mature mice.
authors.affiliation: College of Animal Sci-Tech, Northwest A & F University, Yang Ling, Shaanxi Province, PR China.
authors.forename: Hui Ling
authors.initials: HL
authors.lastname: Zheng
authors.forename: Chao
authors.initials: C
authors.lastname: Yuan
authors.forename: Li Juan
authors.initials: LJ
authors.lastname: Bao
authors.forename: Jun Hui
authors.initials: JH
authors.lastname: An
authors.forename: Zhen Zhen
authors.initials: ZZ
authors.lastname: Zhu
authors.forename: Gong She
authors.initials: GS
authors.lastname: Yang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D015870:Gene Expression; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism* / Q000821:virology; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics* / Q000378:metabolism
chemical_list: C483819:Fosb protein, mouse; D020033:Protein Isoforms; D016760:Proto-Oncogene Proteins c-fos