COX-2 as possible target for the inhibition of PGE2 production by Rikko-san in activated macrophage.
We have previously reported that Rikko-san (RKS) inhibited the lipopolysaccharide (LPS)-stimulated prostaglandin (PG) E2 in mouse macrophage-like RAW264.7 cells without affecting the expression of cyclooxygenase (COX)-2. Here RKS inhibition of the enzyme activity of both COX-1 and COX-2 proteins was investigated. Western blot analysis showed that RKS did not significantly change the S-nitrosylated COX-2 protein level. On the other hand, RKS inhibited the PG production catalyzed by purified COX-2, more effectively than that catalyzed by purified COX-1. These results suggest that RKS inhibits the PGE2 production by selectively inhibiting the COX-2 activity in activated macrophages.
Horie
Norio
N
Division of Pathology, Meikai University School of Dentistry, Sakado, Saitama, Japan.
Hashimoto
Ken
K
Kato
Takao
T
Shimoyama
Tetsuo
T
Kaneko
Tadayoshi
T
Kusama
Kaoru
K
Sakagami
Hiroshi
H
D000818:Animals; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000378:metabolism*; D052246:Cyclooxygenase 2 Inhibitors / Q000494:pharmacology*; D015232:Dinoprostone / Q000096:biosynthesis*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D008262:Macrophage Activation / Q000187:drug effects*; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D009584:Nitrogen / Q000378:metabolism; D013997:Time Factors
D052246:Cyclooxygenase 2 Inhibitors; D004365:Drugs, Chinese Herbal; C502433:rikko-san; D051546:Cyclooxygenase 2; D015232:Dinoprostone; D009584:Nitrogen