title: [Rapid detection of Pseudomonas aernginosa by the fluorescence quantitative TaqMan PCR assay targetting ETA gene].
abstract: Pseudomonas aernginosa (PA) is one of the most universal pathogens in clinical diagnosis, and conventional detection assay has many disadvantages. In this research, a pair of specific primers and a TaqMan fluorescent probe were designed in the conservative region of ETA gene by the method of bioinformatics analysis, the detection method for PA was successfully developed. Different gradient concentrations of PA DNA and various pathogen DNA were amplified by fluorescence quantitative PCR (FQ-PCR) to confirm the specificity and sensitivity of the developed method. Results showed that the developed detection assay is more sensible and specific by comparison to the conventional FQ-PCR method, and it is valuable for research and application prospects.
authors.affiliation: College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xinglong
authors.initials: X
authors.lastname: Xiao
authors.forename: Jingwei
authors.initials: J
authors.lastname: Zhang
authors.forename: Jun
authors.initials: J
authors.lastname: Gong
authors.forename: Yanping
authors.initials: Y
authors.lastname: Pan
authors.forename: Yigang
authors.initials: Y
authors.lastname: Yu
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Wu
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D005098:Exotoxins / Q000235:genetics*; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase; D037521:Virulence Factors / Q000235:genetics*; D000097668:Pseudomonas aeruginosa Exotoxin A
chemical_list: D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D005456:Fluorescent Dyes; D019914:Taq Polymerase; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A