Interplay between MgtC and PagC in Salmonella enterica serovar Typhimurium.
In Salmonella enterica serovar Typhimurium, MgtC and PagC are positively regulated by the PhoP-PhoQ two-component system, which is activated under magnesium deprivation. Both MgtC and PagC are of unknown function but have been involved in intramacrophage survival. We have found that the amount of PagC is lowered in a DeltamgtC mutant strain grown in magnesium depleted medium. However, the effect of MgtC on PagC does not account for the growth defect of a DeltamgtC mutant in macrophages since, in contrast to previous reports, our results indicate that PagC does not contribute to intramacrophage survival. In addition, a pagC null mutant is only poorly attenuated in Nramp1-negative or Nramp1-positive mice. On the other hand, a mgtC null mutant is significantly more attenuated with Nramp1-positive than Nramp1-negative mice, suggesting that a functional Nramp1 (Slc11a1) further limits the multiplication of this mutant within the host.
Alix
Eric
E
Inserm, ESPRI 26, Avenue J.F. Kennedy, 30908 Nîmes Cedex 02, France.
Miki
Tsuyoshi
T
Felix
Christine
C
Rang
Cécile
C
Figueroa-Bossi
Nara
N
Demettre
Edith
E
Blanc-Potard
Anne-Béatrice
AB
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D027682:Cation Transport Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015964:Gene Expression Regulation, Bacterial; D008264:Macrophages / Q000382:microbiology; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008807:Mice, Inbred BALB C; D008809:Mice, Inbred C3H; D009154:Mutation; D012480:Salmonella Infections / Q000382:microbiology*; D012486:Salmonella typhimurium / Q000235:genetics / Q000378:metabolism*; D000099173:Natural Resistance-associated Macrophage Protein 1
D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D008565:Membrane Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1; C066766:pagC protein, Salmonella typhimurium; C093586:MgtC protein, Salmonella typhimurium