Inhibition of acrylamide toxicity in mice by three dietary constituents.
The inhibitory effects of three dietary constituents, tea polyphenols, resveratrol, and diallyl trisulfide, on acrylamide-biomacromolecule (liver DNA, protamine, and hemoglobin) adduct formation at human exposure level were studied by accelerator mass spectrometry. The results demonstrated that the three dietary constituents all significantly inhibited the formation of acrylamide adducts with liver DNA, whereas tea polyphenols and diallyl trisulfide reduced protamine and hemoglobin adducts, respectively. Further biochemical studies showed that acrylamide could significantly inactivate creatine kinase and glutathione S-transferase and deplete glutathione. When the inhibitors were cotreated with acrylamide, all of them could effectively recover the activities of creatine kinase. In addition, tea polyphenols and diallyl trisulfide could increase glutathione S-transferase activity remarkably. On the basis of these results, mechanisms of the effects are discussed. This study might provide a beneficial guide to people's diet for the purpose of reducing the harmful effect of acrylamide.
Xie
Qunying
Q
Department of Chemical Biology, College of Chemistry and Molecular Engineering, State Key Laboratory of Nuclear Physics and Technology, Peking University, Beijing, People's Republic of China. shf@pku.edu.cn
Liu
Ye
Y
Sun
Hongfang
H
Liu
Yuanfang
Y
Ding
Xingfang
X
Fu
Dongpo
D
Liu
Kexin
K
Du
Xianmei
X
Jia
Guang
G
D020106:Acrylamide / Q000037:antagonists & inhibitors* / Q000097:blood / Q000633:toxicity*; D000498:Allyl Compounds / Q000008:administration & dosage*; D000818:Animals; D003402:Creatine Kinase / Q000037:antagonists & inhibitors; D018736:DNA Adducts / Q000032:analysis; D004032:Diet; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005419:Flavonoids / Q000008:administration & dosage*; D005978:Glutathione / Q000032:analysis; D005982:Glutathione Transferase / Q000037:antagonists & inhibitors; D006454:Hemoglobins / Q000737:chemistry; D008099:Liver / Q000737:chemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010636:Phenols / Q000008:administration & dosage*; D059808:Polyphenols; D011479:Protamines / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000008:administration & dosage*; D013440:Sulfides / Q000008:administration & dosage*; D013662:Tea / Q000737:chemistry
D000498:Allyl Compounds; D018736:DNA Adducts; D004791:Enzyme Inhibitors; D005419:Flavonoids; D006454:Hemoglobins; D010636:Phenols; D059808:Polyphenols; D011479:Protamines; D013267:Stilbenes; D013440:Sulfides; D013662:Tea; C042577:diallyl trisulfide; D020106:Acrylamide; D005982:Glutathione Transferase; D003402:Creatine Kinase; D005978:Glutathione; D000077185:Resveratrol