title: Isolation and characterization of a virus from the intracellular green alga symbiotic with Hydra viridis.
abstract: The isolation and characterization of a virus (designated HVCV) from the symbiotic Chlorella-like green alga present in Hydra viridis (Florida strain) are described. The large polyhedral viral particle sedimented at ca. 2600 S in sucrose density gradients and had a buoyant density in CsCl of 1.295 g/ml. The virus contained at least 19 polypeptides (MW range, 10,300 to 82,000) and the major polypeptide (MW, 46,000) was a glycoprotein. The viral genome consisted of a double-stranded DNA with a buoyant density in CsCl of 1.7114 g/ml (52% GC) and a molecular weight of ca. 136 x 10(6).
authors.affiliation: Department of Plant Pathology, University of Nebraska at Lincoln, Lincoln, Nebraska 68583, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Van Etten
authors.forename: R H
authors.initials: RH
authors.lastname: Meints
authors.forename: D E
authors.initials: DE
authors.lastname: Burbank
authors.forename: D
authors.initials: D
authors.lastname: Kuczmarski
authors.forename: D A
authors.initials: DA
authors.lastname: Cuppels
authors.forename: L C
authors.initials: LC
authors.lastname: Lane