Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage.
Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18(+/+) mice and CD18(-/-) -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitrotyrosine immunoreactivity in brains of CD18-knockout mice.
Titova
Elena
E
Department of Physiology and Pharmacology, Loma Linda University, Loma Linda, CA 92354, USA.
Ostrowski
Robert P
RP
Kevil
Christopher G
CG
Tong
Wenni
W
Rojas
Hugo
H
Sowers
Lawrence C
LC
Zhang
John H
JH
Tang
Jiping
J
D000818:Animals; D001929:Brain Edema / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D018821:CD18 Antigens / Q000235:genetics / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009195:Peroxidase / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
D018821:CD18 Antigens; C002744:3-nitrotyrosine; D014443:Tyrosine; D009195:Peroxidase