[Effect of N-acetylcysteine on lipopolysaccharide stimulating IL-8 expression of human uterine smooth cell].
OBJECTIVE
To investigate the effects of N-acetylcysteine (NAC)on the expression of IL-8 and the activity of NF-kappaB, which are induced by lipopolysaccharide (LPS) in human uterine smooth cell.


METHODS
Human uterine smooth cells were primarily cultured and stimulated by LPS after pretreated by 5,10,15 mmol/L NAC and normal saline (NS). ELISA was performed to measure the expression of IL-8 protein in supernatants and RT-PCR was used to detect the IL-8 mRNA expression. The expression of NF-kappaB P65 protein was detected by Western Blotting. Furthermore, the expression of IL-8 mRNA and protein were detected at 0, 3, 6, 12, 24 and 48 h after LPS challenge in human smooth cell pretreated by 10 mmol/L NAC.


RESULTS
(1) NAC significantly inhibited IL-8 mRNA expression and production in human uterine smooth cells with a concentration-dependent way (5-15 mmol/L). (2) The decreasing expression of NF-kappaB P65 protein was accompanied with increasing concentration of NAC in accordance with IL-8 mRNA (P < 0.01).


CONCLUSIONS
NAC may inhibit the induction IL-8 gene and protein by LPS inhibiting NF-kappaB activation in human uterine smooth cell.
Jiang
Xue-Feng
XF
Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.
Zeng
Wei-Yue
WY
Pu
Jie
J
Liu
Yu-Mei
YM
D000111:Acetylcysteine / Q000494:pharmacology*; D015153:Blotting, Western; D002478:Cells, Cultured; D005260:Female; D016166:Free Radical Scavengers / Q000494:pharmacology; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics*; D008070:Lipopolysaccharides / Q000494:pharmacology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051996:Transcription Factor RelA / Q000096:biosynthesis / Q000235:genetics; D014599:Uterus / Q000166:cytology*
D016166:Free Radical Scavengers; D016209:Interleukin-8; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D051996:Transcription Factor RelA; D000111:Acetylcysteine