title: [The formation of BPDE-DNA adduct in rat and its determination by high performance liquid chromatography].
abstract: OBJECTIVE
  To study the benzo (a) pyrene (BaP) induced DNA adduct in rat and establish a method to measure the DNA adduct in blood by high performance liquid chromatography (HPLC).
  
  
  METHODS
  The SD rats were treated with 100 mg/kg of BaP-DMSO (cosolvent: 1% sodium carboxymethyl cellulose) once by i.p., and the blood of femoral vein was collected 5 hours later. The blood DNA was extracted by kit and confirmed by agarose gel electrophoresis. After extraction, the DNA adducts were hydrolyzed in 0.1 nmol/L HCl at 90 degrees C for 4 hours. The acid-hydrolysis products (BP-tetrols) of DNA adducts were extracted by ethyl acetate and measured by HPLC, and finally confirmed by HPLC-MS.
  
  
  RESULTS
  In chromatogram there were new peaks to occur for rats treated by BaP, with compared to control. By HPLC-MS, one of the new peaks was confirmed to be BP-tetrol.
  
  
  CONCLUSIONS
  The rats ingesting BaP in the way described in this experiment can form DNA adducts, and the adducts in blood can be detected by HPLC.
authors.affiliation: Department of Sanitary Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
authors.forename: Liu-Hua
authors.initials: LH
authors.lastname: Yang
authors.forename: Qu
authors.initials: Q
authors.lastname: Liu
authors.forename: Cheng-Jun
authors.initials: CJ
authors.lastname: Sun
authors.forename: Shu-Yan
authors.initials: SY
authors.lastname: Guo
authors.forename: Shao-Jie
authors.initials: SJ
authors.lastname: Lin
mesh_terms: D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000737:chemistry; D000818:Animals; D001564:Benzo(a)pyrene / Q000737:chemistry / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004247:DNA / Q000097:blood / Q000737:chemistry; D018736:DNA Adducts / Q000097:blood* / Q000737:chemistry; D004249:DNA Damage; D004587:Electrophoresis, Agar Gel; D005260:Female; D008297:Male; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018736:DNA Adducts; C043931:benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA; D001564:Benzo(a)pyrene; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D004247:DNA