title: Comparison of naphthalene bioavailability determined by whole-cell biosensing and availability determined by extraction with Tenax.
abstract: A rapid biological method for the determination of the bioavailability of naphthalene was developed and its value as an alternative to extraction-based chemical approaches demonstrated. Genetically engineered whole-cell biosensors are used to determine bioavailable naphthalene and their responses compared with results from Tenax extraction and chemical analysis. Results show a 1:1 correlation between biosensor results and chemical analyses for naphthalene-contaminated model materials and sediments, but the biosensor assay is much faster. This work demonstrates that biosensor technology can perform as well as standard chemical methods, though with some advantages including the inherent biological relevance of the response, rapid response time, and potential for field deployment. A survey of results from this work and the literature shows that bioavailability under non-equilibrium conditions nonetheless correlates well with K(oc) or K(d). A rationale is provided wherein chemical resistance is speculated to be operative.
authors.affiliation: Swiss Federal Institute of Technology (EPFL), Laboratory of Soil Science, CH-1015 Lausanne, Switzerland.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Kohlmeier
authors.forename: Matthew
authors.initials: M
authors.lastname: Mancuso
authors.forename: Upul
authors.initials: U
authors.lastname: Deepthike
authors.forename: Robin
authors.initials: R
authors.lastname: Tecon
authors.forename: Jan Roelof
authors.initials: JR
authors.lastname: van der Meer
authors.forename: Hauke
authors.initials: H
authors.lastname: Harms
authors.forename: Mona
authors.initials: M
authors.lastname: Wells
mesh_terms: D001682:Biological Availability; D015374:Biosensing Techniques / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D005740:Gases; D049452:Green Fluorescent Proteins / Q000032:analysis / Q000378:metabolism; D008856:Microscopy, Fluorescence; D009281:Naphthalenes / Q000032:analysis / Q000493:pharmacokinetics*; D011108:Polymers; D016958:Pseudomonas putida / Q000378:metabolism; D052616:Solid Phase Extraction / Q000379:methods; D014873:Water Pollutants / Q000032:analysis / Q000493:pharmacokinetics*
chemical_list: D005740:Gases; D009281:Naphthalenes; D011108:Polymers; D014873:Water Pollutants; D049452:Green Fluorescent Proteins; C031721:naphthalene; C010514:tenax