title: Plant and animal transglutaminases: do similar functions imply similar structures?
abstract: In plants the post-translational modification of proteins by polyamines catalysed by transglutaminases has been studied since 1987; it was identified by the production of glutamyl-polyamine derivatives, biochemical features, recognition by animal antibodies and modification of typical animal substrates. Transglutaminases are widespread in all plant organs and cell compartments studied until now, chloroplast being the most studied. Substrates are: photosynthetic complexes and Rubisco in chloroplasts, cytoskeleton and cell wall proteins. Roles either specific of plants or in common with animals are related to photosynthesis, fertilisation, stresses, senescence and programmed cell death, showing that the catalytic function is conserved across the kingdoms. AtPng1p, the first plant transglutaminase sequenced shows undetectable sequence homology to the animal enzymes, except for the catalytic triad. It is, however, endowed with a calcium-dependent activity that allowed us to build a three-dimensional model adopting as a template the animal transglutaminase 2.
authors.affiliation: Unit of Plant Cell Growth and Differentiation, Dipartimento di Biologia Evoluzionistica Sperimentale, Sede Botanica, University of Bologna, via Irnerio 42, 40126, Bologna, Italy. donatella.serafini@unibo.it
authors.forename: Donatella
authors.initials: D
authors.lastname: Serafini-Fracassini
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Della Mea
authors.forename: Gianluca
authors.initials: G
authors.lastname: Tasco
authors.forename: Rita
authors.initials: R
authors.lastname: Casadio
authors.forename: Stefano
authors.initials: S
authors.lastname: Del Duca
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002118:Calcium / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D008969:Molecular Sequence Data; D010944:Plants / Q000201:enzymology*; D017386:Sequence Homology, Amino Acid; D011503:Transglutaminases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D011503:Transglutaminases; D002118:Calcium