title: Cellular clot formation in a sipunculan worm: entrapment of foreign particles, cell death and identification of a PGRP-related protein.
abstract: Clotting in animals having open or closed circulatory system comprises humoral and cellular mechanisms. Sipunculans are a small phylum of non-segmented marine worms that do not have a true circulatory system. These worms can form a cellular clot without transforming cell-free coelomic fluid into an insoluble mass. The clot may also contribute to immune response by entrapping foreign particles. We evaluated the formation of a cellular clot ex vivo in the sipunculan Themiste petricola after activation through glass contact and sea water, the ability to entrap magnetic beads and non-pathogen cyanobacteria particles within the clot, and the presence of a peptidoglycan recognition protein S (PGRP-S) antigen in cells forming the clot. Our results showed that the clot was formed by homotypic aggregation of large granular leukocytes (LGLs), a subtype of cells found in the coelomic fluid. Aggregated LGLs served to entrap magnetic beads and non-pathogen cyanobacteria particles, and PGRP-S antigen was detected both in non-activated LGLs and in activated homotypic aggregates through immunofluorescence, Western blot and flow cytometry. Cellular clots were found to be positive to Annexin V-FITC labelling. Complete disintegration of cytoplasm with shedding of microparticles, nuclear isolation and degradation were also observed by light microscopy and flow cytometry. In conclusion, cellular clot formation in Themiste petricola may serve both haemostatic and immune functions entailing rapid activation changes in LGLs, entrapment of foreign particles within a homotypic aggregate, and further cell death.
authors.affiliation: Department of Immunology, IDEHU-National Research Council, School of Pharmacy and Biochemistry, University of Buenos Aires, Buenos Aires, Junín 956 4to piso, Capital Federal 1113, Argentina. gblanco@ffyb.uba.ar
authors.forename: Guillermo A
authors.initials: GA
authors.lastname: Blanco
authors.forename: Emilio L
authors.initials: EL
authors.lastname: Malchiodi
authors.forename: Mauricio C
authors.initials: MC
authors.lastname: De Marzi
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002352:Carrier Proteins / Q000276:immunology*; D016923:Cell Death / Q000502:physiology; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D007962:Leukocytes / Q000276:immunology*; D009348:Nematoda / Q000276:immunology*
chemical_list: D002352:Carrier Proteins; C118897:peptidoglycan recognition protein