title: [Synthesis and mass spectrometric analysis of aristolochic acid-deoxyguanosine adducts].
abstract: To synthesize aristolochic acid (AA)-2'-deoxyguanosine 5'-monophosphate (dGp) adducts in vitro and develop a novel method for the characterization of the adducts using multiple mass spectrometric techniques. AA was incubated with dGp in vitro using either enzymatic activation (by xanthine oxidase) or chemical activation (by zinc) to synthesize AA-dGp adducts, and the reaction conditions were optimized. Crude extracts were analyzed by techniques of liquid chromatography-electrospray ionization/tandem mass spectrometry (LC-MS/MS) and high accuracy mass data and isotope pattern of super high resolution Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICRMS). The quasi-molecular ion peaks of the AA-dGp adducts were obtained in the negative ion mode. Analysis by electrospray ionization/tandem mass spectrometry (ESI-MS/MS) provided useful structural information about AA-dGp adducts. AA can bind covalently to the exocyclic amino group of deoxyguanosine to form AA-dGp adducts. MS analysis is a powerful tool to detect and identify AA-dGp adducts simply, rapidly and accurately.
authors.affiliation: China-Japan Friendship Hospital, Beijing 100029, China.
authors.forename: Wen-Xuan
authors.initials: WX
authors.lastname: Ji
authors.forename: Mi-Xin
authors.initials: MX
authors.lastname: Liu
authors.forename: Cheng-Dui
authors.initials: CD
authors.lastname: Yang
authors.forename: Yi-Pu
authors.initials: YP
authors.lastname: Chen
mesh_terms: D034341:Aristolochic Acids / Q000138:chemical synthesis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D018736:DNA Adducts / Q000138:chemical synthesis*; D003849:Deoxyguanosine / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D034341:Aristolochic Acids; D018736:DNA Adducts; D004247:DNA; C000228:aristolochic acid I; D003849:Deoxyguanosine