title: The nuclear exosome and adenylation regulate posttranscriptional tethering of yeast GAL genes to the nuclear periphery.
abstract: GAL genes and other activated yeast genes remain tethered to the nuclear periphery even after transcriptional shutoff. To identify factors that affect this tethering, we designed a plasmid-based visual screen. Although many factors affected GAL tethering during transcription, fewer specifically affected posttranscriptional tethering. Tw o of these, Rrp6p and Lrp1p, are nuclear exosome components known to contribute to RNA retention near transcription sites (dot RNA). Moreover, these exosome mutations lead to a substantial posttranscriptional increase in polyadenylated GAL1 3' ends. This accompanies a loss of unadenylated (pA-) GAL1 RNA and a loss of posttranscriptional gene-periphery tethering, as well as a decrease in dot RNA levels. This suggests that the exosome inhibits adenylation of some GAL1 transcripts, which results in the accumulation of pA- RNA adjacent to the GAL1 gene. We propose that this dot RNA, probably via RNP proteins, contributes to the physical tether linking the GAL1 gene to the nuclear periphery.
authors.affiliation: Department of Biology, Brandeis University, Waltham, MA 02454, USA.
authors.forename: Sadanand
authors.initials: S
authors.lastname: Vodala
authors.forename: Katharine Compton
authors.initials: KC
authors.lastname: Abruzzi
authors.forename: Michael
authors.initials: M
authors.lastname: Rosbash
mesh_terms: D001483:Base Sequence; D001681:Biological Assay; D002467:Cell Nucleus / Q000235:genetics*; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; D005686:Galactokinase / Q000235:genetics* / Q000378:metabolism; D015966:Gene Expression Regulation, Fungal; D005800:Genes, Fungal; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D011061:Poly A / Q000378:metabolism; D026723:Polyadenylation / Q000235:genetics*; D016337:RNA, Catalytic / Q000378:metabolism; D012331:RNA, Fungal / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D013379:Substrate Specificity; D014158:Transcription, Genetic; D015533:Transcriptional Activation / Q000235:genetics
chemical_list: D016337:RNA, Catalytic; D012331:RNA, Fungal; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D029701:Saccharomyces cerevisiae Proteins; D049452:Green Fluorescent Proteins; D011061:Poly A; C524219:GAL1 protein, S cerevisiae; D005686:Galactokinase; D005095:Exoribonucleases; D063326:Exosome Multienzyme Ribonuclease Complex; C112610:RRP6 protein, S cerevisiae