Virus-specific RNA species present in the cytoplasm of Rous sarcoma virus-infected chicken cells.
Polyadenylated and nonpolyadenylated cytoplasmic RNA from chicken cells infected with wild-type (wt) and transformation-defective (td) Rous sarcoma virus (RSV) were fractionated on sucrose density gradients and hybridized with a [3H]cDNA probe complementary to RSV-RNA to determine the size distribution of virus-specific RNA. Two major species of poly (A) -containing viral RNA sedimenting at 35 and 21 S were detected in wt and td virus-infected cells. Both RNA classes were released from polyribosomes with puromycin, suggesting that each RNA species serves as messenger in vivo. Another minor size class of virus-specific RNA, 10-12 S, was found in both the polyadenylated and nonpolyadenylated RNA fractions.
Brugge
J S
JS
Department of Pathology, University of Colorado Medical Center, Denver, Colorado 80262, USA.
Purchio
A F
AF
Erikson
R L
RL