title: Rapid renaturation of denatured and aggregated proteins using liquid paraffin as a pseudolipid bilayer membrane.
abstract: A means of rapidly renaturing denatured protein was devised and evaluated. Three liquids were laminarly layered in a centrifuge tube, in which two solutions sandwiched liquid paraffin so as to form a pseudolipid bilayer. Denatured and aggregted protein placed on the upper surface of liquid paraffin was renatured as it passed through liquid-paraffin layer into the renaturation buffer during the centrifugation. The aggregated and denatured protein selectively passed through the liquid-paraffin layer, whereas other solutions, such as chaotropic agents or organic solvent, could not. This means that a rapid dilution condition favorable for protein renaturation was realized in a small scale. Aggregated and denatured BSA and ribonuclease A were renatured and resolubilized as they passed through the liquid-paraffin layer into an appropriate renaturation buffer solution. This method was also applied to the rapid heme reconstitution of myoglobin from Feprotoporphyrin IX to Zn-protoporphyrin IX. (c) 1994 John Wiley & Sons, Inc.
authors.affiliation: Department of Biochemical Engineering, Toyama National College of Technology, Toyama 939, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Yoshii
authors.forename: T
authors.initials: T
authors.lastname: Kojima
authors.forename: T
authors.initials: T
authors.lastname: Furuta
authors.forename: A
authors.initials: A
authors.lastname: Yasunishi