WELYSSA: a simple tool using mouse monoclonal antibodies for the detection of lyssavirus nucleocapsid in rabies suspected specimens.
A Monoclonal antibody (MAb)-based capture enzyme-linked immunosorbent assay (ELISA) named WELYSSA was developed for the diagnosis of rabies suspected specimens using prototype viruses from the different genotypes of lyssavirus and from various geographic origins and phylogenetic lineages. It included a panel of 1,660 specimens received for rabies diagnostic testing, and was found to be highly specific (99.9%) and sensitive (97.0%) when compared to other recommended rabies diagnostic methods.
Xu
G
G
Wuhan Institute of Biological Products, Wuhan, Hubei Province, China. xugelin@yahoo.com.cn
Weber
P
P
Hu
Q
Q
Xue
H
H
Audry
L
L
Li
C
C
Wu
J
J
Bourhy
H
H
D000818:Animals; D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral / Q000097:blood; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods* / Q000592:standards; D005838:Genotype; D006801:Humans; D018114:Lyssavirus / Q000276:immunology*; D019251:Nucleocapsid / Q000032:analysis* / Q000276:immunology; D011818:Rabies / Q000175:diagnosis* / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral