Increased protein turnover and proteolysis is an early and primary feature of short-term experimental hyperthyroidism in healthy women.
BACKGROUND
Hyperthyroidism increases energy expenditure, glucose turnover, lipolysis, and protein breakdown.


OBJECTIVE
Our objective was to test whether increased protein breakdown occurs independently of other catabolic effects in mild experimental hyperthyroidism.


METHODS
We conducted a single-blind, randomized, placebo-controlled, crossover study. Protein dynamics of the whole body and of the forearm muscles were measured by amino acid tracer dilution technique ([(15)N]phenylalanine and [(2)H(4)]tyrosine). All subjects underwent a 3-h study in the basal state followed by a 3-h euglycemic clamp study.


METHODS
The study took place at a university clinical research unit.


METHODS
Eight healthy women (24-46 yr old) participated.


METHODS
Intervention included 6 d thyroid hormone (T(4) 50 microg and T(3) 0.67 microg/kg.d) or placebo administration.


RESULTS
Thyroid hormone administration led to mild T(3) hyperthyroidism with more than a doubling of T(3) levels and suppression of TSH. Energy expenditure and body composition was unchanged. Glucose infusion rates, forearm glucose uptake, and levels of lipid intermediates were also alike. Basal whole-body phenylalanine flux and tyrosine flux (reflecting whole-body protein breakdown) were increased (P < 0.05) as were whole-body protein synthesis rate (P = 0.05). Basal forearm rate of appearance and disappearance for phenylalanine (reflecting muscle protein breakdown and synthesis) were similar.


CONCLUSIONS
Mild short-term experimental hyperthyroidism increases whole-body protein turnover and breakdown before any measurable changes in energy expenditure or glucose and fat metabolism, suggesting that amino acid and protein metabolism is an early and primary target for thyroid hormone action in humans.
Riis
Anne Lene Dalkjaer
AL
Medical Department M, Aarhus University Hospital, 8000 Aarhus C, Denmark. anne.lene.riis@ki.au.dk
Jørgensen
Jens Otto Lunde
JO
Ivarsen
Per
P
Frystyk
Jan
J
Weeke
Jørgen
J
Møller
Niels
N
D000328:Adult; D000596:Amino Acids / Q000032:analysis / Q000378:metabolism; D001823:Body Composition / Q000187:drug effects; D018592:Cross-Over Studies; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D006262:Health; D006801:Humans; D006980:Hyperthyroidism / Q000139:chemically induced* / Q000378:metabolism* / Q000473:pathology; D007333:Insulin Resistance; D008875:Middle Aged; D009132:Muscles / Q000737:chemistry / Q000187:drug effects; D010919:Placebos; D011499:Protein Processing, Post-Translational / Q000187:drug effects / Q000502:physiology*; D011506:Proteins / Q000187:drug effects / Q000378:metabolism*; D016037:Single-Blind Method; D013963:Thyroid Hormones / Q000009:adverse effects* / Q000494:pharmacology; D013997:Time Factors; D015854:Up-Regulation
D000596:Amino Acids; D010919:Placebos; D011506:Proteins; D013963:Thyroid Hormones