Kinetics of quinone inactivation of Tulare apple mosaic virus.
A direct assay technique was used to measure the kinetics of o-quinone inactivation of Tulare apple mosaic virus (TAMV). Inactivation occurred at rates measurable in seconds, minutes, or hours depending upon the particular quinone tested and the ratio of benzoquinone equivalents per milligram of virus used. The inactivation rate appeared independent of the absolute virus concentration. Inactivation rates were not linear at any quinone concentration measurable. However, inactivation data plotted according to the equation 1/I = (b/a)(1/t) + 1/a where I is the percent inactivation, a and b constants, and t exposure time, gave linear curves from which initial inactivation rates (percent virus inactivated in the first second) and minimum exposure times (times required to cause 100% inactivation) could be determined.
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Department of Plant Pathology, Washington State University Irrigated Agriculture Research and Extension Center, Prosser, Washington 99350, USA.