title: Expression of Dm-AMP1 in rice confers resistance to Magnaporthe oryzae and Rhizoctonia solani.
abstract: Magnaporthe oryzae and Rhizoctonia solani, are among the most important pathogens of rice, severely limiting its productivity. Dm-AMP1, an antifungal plant defensin from Dahlia merckii, was expressed in rice (Oryza sativa L. sp. indica cv. Pusa basmati 1) using Agrobacterium tumefaciens-mediated transformation. Expression levels of Dm-AMP1 ranged from 0.43% to 0.57% of total soluble protein in transgenic plants. It was observed that constitutive expression of Dm-AMP1 suppresses the growth of M. oryzae and R. solani by 84% and 72%, respectively. Transgenic expression of Dm-AMP1 was not accompanied by an induction of pathogenesis-related (PR) gene expression, indicating that the expression of DmAMP1 directly inhibits the pathogen. The results of in vitro, in planta and microscopic analyses suggest that Dm-AMP1 expression has the potential to provide broad-spectrum disease resistance in rice.
authors.affiliation: Department of Microbiology and Biotechnology Centre, The M S. University of Baroda, Vadodara, Gujarat, 390002, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Jha
authors.forename: Harsukh G
authors.initials: HG
authors.lastname: Tank
authors.forename: Bishun Deo
authors.initials: BD
authors.lastname: Prasad
authors.forename: Bharat B
authors.initials: BB
authors.lastname: Chattoo
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology; D015152:Blotting, Northern; D015139:Blotting, Southern; D015153:Blotting, Western; D023082:Defensins / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D007113:Immunity, Innate; D007124:Immunoenzyme Techniques; D020082:Magnaporthe / Q000472:pathogenicity*; D012275:Oryza / Q000235:genetics* / Q000276:immunology / Q000382:microbiology*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology / Q000382:microbiology*; D010940:Plant Proteins / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000382:microbiology*; D012232:Rhizoctonia / Q000472:pathogenicity*; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000276:immunology / Q000378:metabolism
chemical_list: D000935:Antifungal Agents; D023082:Defensins; C099969:Dm-AMP1 protein, Dahlia merckii; D010940:Plant Proteins