Seed defensins of barnyard grass Echinochloa crusgalli (L.) Beauv.
From the annual weed barnyard grass Echinochloa crusgalli (L.) Beauv., two novel defensins Ec-AMP-D1 and Ec-AMP-D2 that differ by a single amino acid substitution were isolated by a combination of different chromatographic procedures. Both defensins were active against several phytopathogenic fungi and the oomycete Phytophthora infestans at micromolar concentrations. The Ec-AMP-D1 showed higher activity against the oomycete than Ec-AMP-D2. The comparison of the amino acid sequences of the antifungal E. crusgalli defensins with those of earlier characterized T. kiharae defensins [T.I. Odintsova, Ts.A. Egorov, A.Kh. Musolyamov, M.S. Odintsova, V.A. Pukhalsky, E.V. Grishin, Seed defensins from T. kiharae and related species: genome localization of defensin-encoding genes, Biochimie, 89 (2007) 605-612.] that were devoid of substantial antifungal activity point to the C-terminal region of the molecule as the main determinant of the antifungal activity of E. crusgalli defensins.
Odintsova
Tatyana I
TI
Laboratory of Plant Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russian Federation.
Rogozhin
Eugene A
EA
Baranov
Yurij
Y
Musolyamov
Alexander Kh
AKh
Yalpani
Nasser
N
Egorov
Tsezi A
TA
Grishin
Eugene V
EV
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D023082:Defensins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D031747:Echinochloa / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D055750:Phytophthora infestans / Q000187:drug effects / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment
D000890:Anti-Infective Agents; D000935:Antifungal Agents; D023082:Defensins