[Assessment of the susceptibility of the gram-negative rods isolated from hospitalized patients to cefoperazone/sulbactam].
The susceptibility to cefoperazone/sulbactam of 197 strains of Gram-negative rods demonstrating an ESBL-positive phenotype was determined. The assortment of the investigated strains was as follows (numbers of strains are given in the brackets): E. cloacae (63), S. marcescens (46), K. pneumoniae (21), P. mirabilis (17), E. coli (9), P. vulgaris (8), P. aeruginosa (20) and A. baumanni (13). 83 strains from 197 were susceptible (42.1%). The MIC values were determined and the disc-diffusion method was performed. The susceptibilities among particular species were as follows (the order of data in the brackets is: % of the susceptible strains/MIC50/MIC90): E. cloacae (54.0/16/64), S. marcescens (23.9/64/> or = 128), K. pneumoniae (38.1/32/64), P. mirabilis (41.2/32/64), E. coli (44.4/32/32), P. vulgaris (75.0/8/32), P. aeruginosa (35.0/32/64), A. baumannii (46.2/32/64). Using disc-diffusion method, for 184 strains the difference between diameter of the inhibition zone around the disc with cefoperazone and the disc with cefoperazone/sulbactam was calculated. This difference amounted 5 mm or more in the case of 76.6% of the investigated strains. The results indicate that the comparison of the inhibition zones around cefoperazone and cefoperazone/sulbactam discs may be an additional method useful for phenotypic detection of ESBL producing organisms. These results highly correlated with results obtained by using analogous test with cefpirome and cefpirome/clavulanic acid (85.6% of concordance).
Młynarczyk
Grazyna
G
Katedra i Zakład Mikrobiologii Lekarskiej Warszawskiego Uniwersytetu medycznego w Warszawie.
Młynarczyk
Andrzej
A
Wysocka
Patricia Castaneda
PC
Scibisz
Anna
A
Kostyra
Kacper
K
Sańczyk
Ewa
E
Jezewska
Grazyna
G
Sliwa
Robert
R
Winek
Katarzyna
K
Wolska
Magdalena
M
Pastuszak
Andrzej
A
Serafin
Iwona
I
Łuczak
Mirosław
M
D000890:Anti-Infective Agents / Q000494:pharmacology*; D002438:Cefoperazone / Q000494:pharmacology*; D018550:Cephalosporin Resistance; D052978:Disk Diffusion Antimicrobial Tests; D024901:Drug Resistance, Multiple, Bacterial; D016946:Gram-Negative Aerobic Rods and Cocci / Q000187:drug effects*; D006801:Humans; D013407:Sulbactam / Q000494:pharmacology*; D001618:beta-Lactamases / Q000096:biosynthesis
D000890:Anti-Infective Agents; D002438:Cefoperazone; D001618:beta-Lactamases; D013407:Sulbactam