Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior.
Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium-mediated transient expression in Nicotiana excelsior was applied for quick preparative production of recombinant GFP. The protein purification scheme has been developed and included ammonium sulfate precipitation and Q-sepharose anion-exchange chromatography. It results in obtaining of a fraction with about 85% GFP homogeneity and the protein yield of about 75%.
Sindarovska
Y R
YR
Institute of Cell Biology and Genetic Engineering, Kyiv, Ukraine.
Sheludko
Y V
YV
Gerasymenko
I M
IM
Bannikova
M A
MA
Kuchuk
N V
NV
D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins