Loss of heterozygosity of the APC gene in oral squamous cell carcinoma.
The aim of this study was to investigate loss of heterozygosity (LOH) of the APC tumor suppressor gene loci, using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) in 40 cases of oral squamous cell carcinoma (OSCC). Observed informativity was 72.5% for APC exon 11 and 82.5% for APC exon 15. LOH at APC exon 11 was observed in 2 (6.9%) of 29 informative cases, and no LOH was observed for APC exon 15. Our results suggest that inactivation of the APC gene plays a minor role in the carcinogenesis of OSCC.
Rivero
Elena R C
ER
Department of Pathology, Health Science Center, Federal University of Santa Catarina, CEP 88040-370, Florianópolis, SC, Brazil. riet@ccs.ufsc.br
Horta
Martinho C R
MC
Silva Guerra
Eliete N
EN
Ferraz
Alberto R
AR
Nunes
Fábio D
FD
D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D017491:Genes, APC; D006801:Humans; D019656:Loss of Heterozygosity; D009062:Mouth Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length