[Induced apoptosis and mechanism of endosulfan in mouse germ cells].
Endosulfan which is going to be included as POPs in effect of organism have become the focus of international concern. To explore endosulfan on apoptosis of spermatogenic cells and mechanisms, doses of 7.0, 3.5 and 1.75 mg/kg oral administration to mice exposed to 28 consecutive days. Morphology characters of spermatogenic cells observed by laser confocal scanning microscope (LCSM), DNA ladder detected by electricity swims, and increasing of DNA degeneration rate measured through diphenylamine (p < 0.01). Endosulfan might enhance calcium concentration in the spermatogenic cells and restrained Na+ K+ -ATPase and Ca2+ Mg2+ -ATPase activities that showed endosulfan might induce spermatogenic cells in the testicle to occur apoptosis (p < 0.01), to cause the function of transporting Ca2+ to be limited, reduce the ability of excluding calcium and send Ca2+ concentration to hoist sustainingly,i.e. calcium overload in the cells.
Ren
Nan-qi
NQ
College of Civil Environmental Engineering, Harbin University of Technology, Harbin 150090, China. rnq@hit.edu.cn
Zhang
Xiao-dan
XD
Zhou
Guang-hong
GH
Chen
Shao-jun
SJ
D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004726:Endosulfan / Q000008:administration & dosage / Q000633:toxicity*; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity; D008297:Male; D051379:Mice; D011897:Random Allocation; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
D007306:Insecticides; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D004726:Endosulfan; D002118:Calcium