Microarray analysis of bacterial gene expression: towards the regulome.
Microarray technology allows co-regulated genes to be identified. In order to identify genes that are controlled by specific regulators, gene expression can be compared in mutant and wild-type bacteria. However, there are a number of pitfalls with this approach; in particular, the regulator may not be active under the conditions in which the wild-type strain is cultured. Once co-regulated genes have been identified, proteinbinding motifs can be identified. By combining these data with a map of promoters, or operons (the operome), the regulatory networks in the cell (the regulome) can start to be built up.
Kendall
Sharon L
SL
Department of Pathology and Infectious Diseases, Royal Veterinary College, Royal College Street, London NW1 0TU, UK. skendall@rvc.ac.uk
Movahedzadeh
Farahnaz
F
Wietzorrek
Andreas
A
Stoker
Neil G
NG