title: Axial coding in full-field microscopy using three-dimensional structured illumination implemented with no moving parts.
abstract: We report a simple optical setup to produce both axial and lateral structured illumination through a single objective lens. With a minimum of six full-field images obtained without moving either the sample or the microscope objective, 100 nm diameter fluorescent beads can be localized axially with an accuracy of 50 nm in a 1.76-microm-thick layer. We show that this axial localization improvement can easily be combined with classical lateral structured illumination, so that lateral resolution enhancement by a factor of 2 is maintained.
authors.affiliation: Laboratoire Photons Et Matière, CNRS, Unité Propre de Recherche 5, Ecole Supérieure de Physique et Chimie, Paris, France.
authors.forename: Pedro Felipe Gardeazábal
authors.initials: PF
authors.lastname: Rodríguez
authors.forename: Eduardo
authors.initials: E
authors.lastname: Sepulveda
authors.forename: Benoit
authors.initials: B
authors.lastname: Dubertret
authors.forename: Vincent
authors.initials: V
authors.lastname: Loriette