[In vivo study on the potential differention capabilities of human peripheral blood-derived mononuclear cells transplanted into the nude mice with myocardial infarction].
The purpose of this study was to observe whether human peripheral dervied monouncleas cells (hMNCs) could participate in the regeneration process of the ischemic hearts in the way of differentiating into cardiomyocytes, vascular endothelial cells and smooth muscle cells. hMNCs were transplanted into the bodies of the mice with myocardial infarction through the tail vein injection. Hearts were harvested 2-12 weeks after injection then sliced up into frozen sections of 5 micron thickness. Double immunofluorescence staining was used to test the differentiation of the grafted cells into cardiomyocytes, smooth muscle cells and vascular endothelial cells which revealed that cells expressing both HLA and TNT, HLA and alpha-SMA, HLA and vWF existed in the hearts of the mice. According to the study, it is probable that hMNCs could participate in the regeneration process of the infarcted hearts in the way of differentiation.
Zhang
Zhi
Z
Department of Cardiology, Affiliated Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China.
Yan
Yiwen
Y
Zhu
Yanqi
Y
ZhuGe
Yin
Y
Dai
Qiuyan
Q
Sun
Baogui
B
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000637:transplantation*; D051379:Mice; D008819:Mice, Nude; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D032383:Myocytes, Cardiac / Q000166:cytology*; D014183:Transplantation, Heterologous