title: Automated cerebrospinal fluid cytology.
abstract: OBJECTIVE
  To evaluate the Abbott CELL-DYN Sapphire cytometer for cerebrospinal fluid (CSF) cell count and differentiation.
  
  
  METHODS
  One hundred three analyses of CSF cells by the CELL-DYN Sapphire were compared with routine cell count and microscopic differentiation and correlation coefficients calculated.
  
  
  RESULTS
  The total cell count of both methods correlated well. The detection of erythrocytes was good (0.898), and a higher content of erythrocytes >100/microL had little effect on total leukocyte count. The correlation between both methods was best with higher leukocyte counts >25/microL (r=0.987), whereas at cell counts <25/microL, the correlation was considerably less precise (r=0.613). For the differentiation of cells, lymphocytes and neutrophils showed moderate correlation. The results for monocytes and eosinophils did not correlate.
  
  
  CONCLUSIONS
  The results for the total cell count in this study are comparable with those achieved with the Bayer Advia 120. While the Abbott CELL-DYN Sapphire yielded slightly better results for erythrocytes and total cell count with a higher erythrocyte content, the Advia 120 achieved slightly better results of lymphocyte and neutrophil count in a previous study.
authors.affiliation: Department of Clinical Chemistry, University of Göttingen, Göttingen, Germany.
authors.forename: Tanja
authors.initials: T
authors.lastname: Heller
authors.forename: Ingelore
authors.initials: I
authors.lastname: Nagel
authors.forename: Bettina
authors.initials: B
authors.lastname: Ehrlich
authors.forename: Mathias
authors.initials: M
authors.lastname: Bähr
authors.forename: Herwig
authors.initials: H
authors.lastname: Strik
mesh_terms: D001772:Blood Cell Count / Q000295:instrumentation / Q000379:methods; D002555:Cerebrospinal Fluid / Q000166:cytology*; D005434:Flow Cytometry / Q000295:instrumentation* / Q000379:methods; D006801:Humans; D008853:Microscopy / Q000379:methods; D015203:Reproducibility of Results