A simple and effective method to overcome the inhibition of Fe to PCR.
Fe inhibition to PCR can be overcome by adding greatly excessive EDTA first, and then the same amount Mg(2+) as EDTA to PCR mix. By this method impure DNA was amplified successfully. DNA samples containing metals whose complexation constant with EDTA was much higher than Mg(2+) can also be amplified.
Teng
Fei
F
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China.
Guan
Yuntao
Y
Zhu
Wanpeng
W
D018441:Biofilms; D002614:Chelating Agents / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D004492:Edetic Acid / Q000737:chemistry; D007501:Iron / Q000737:chemistry* / Q000494:pharmacology*; D008274:Magnesium / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000187:drug effects / Q000379:methods*
D002614:Chelating Agents; D004247:DNA; D004492:Edetic Acid; D007501:Iron; D008274:Magnesium