Overexpression of CXC chemokine receptors is required for the superior glioma-tracking property of umbilical cord blood-derived mesenchymal stem cells.
Our observations indicate that umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) have a strong migration capacity toward the human glioma cell line, U-87 MG, LN18, U138, and U251, when compared to several other cancer cell lines. In order to identify soluble factors that function to attract UCB-MSCs, we used cytokine antibody arrays to screen changed cytokines in conditioned media from U-87 MG cells. Among these, interleukin-8 (IL-8) and growth-related oncogene (GRO-alpha) enhanced UCB-MSC migration. Furthermore, antibodies treatment against the IL-8 receptors reduced these migration events and overexpression of IL-8 in cells with lower level of IL-8 such as A549 could induce UCB-MSC migration. Since we found that the capacity of UCB-MSC migration is much higher than that of bone marrow-derived MSCs (BM-MSCs) toward either U-87 MG cells or recombinant IL-8, we compared the levels of the IL-8 receptor, CXC chemokine receptor 1 (CXCR1) and CXCR2 between two kinds of MSCs by RT-PCR and immunostaining. Expression levels of two receptors were much higher in UCB-MSCs than in BM-MSCs. These data suggest that higher levels of two IL-8 receptors could influence downstream signaling events affecting superior UCB-MSC migration toward the glioma cells.
Kim
Dal-Soo
DS
Biomedical Research Institute, MEDIPOST Co., Ltd., Republic of Korea.
Kim
Ji Hyun
JH
Lee
Jae Kwon
JK
Choi
Soo Jin
SJ
Kim
Jae-Sung
JS
Jeun
Sin-Soo
SS
Oh
Wonil
W
Yang
Yoon Sun
YS
Chang
Jong Wook
JW
D045744:Cell Line, Tumor; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D005312:Fetal Blood / Q000166:cytology*; D005910:Glioma / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D016209:Interleukin-8 / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology*; D054387:Receptors, CXCR / Q000235:genetics / Q000378:metabolism*; D053700:Receptors, Interleukin-8 / Q000235:genetics / Q000378:metabolism
D016209:Interleukin-8; D054387:Receptors, CXCR; D053700:Receptors, Interleukin-8