Review of molecular modification techniques for improved detection of biomolecules by mass spectrometry.
After a soft ionizing method was established, MS (mass spectrometry) has become a more common tool in biochemistry because soft ionization made it possible to detect large molecules such as proteins. Many kinds of applications were established to further utilize MS for the identification or quantitation of biomolecules. In this review, we introduce recent applications with special focus on chemical modification techniques and chemical probes developed for the MS determination of biomolecules.
Honda
Aki
A
Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku, Yokohama, Kanagawa 223-8522, Japan.
Suzuki
Yoshio
Y
Suzuki
Koji
K
D004247:DNA / Q000032:analysis* / Q000737:chemistry; D007118:Immunoassay; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000295:instrumentation / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000737:chemistry
D011506:Proteins; D004247:DNA