title: Archvillin anchors in the Z-line of skeletal muscle via the nebulin C-terminus.
abstract: Z-Line of skeletal muscle is a complex protein network that likely plays an important role in signaling and muscle homeostasis. We used the yeast two-hybrid system to search for potential novel ligands of the Z-line portion of nebulin. We found that the C-terminal region of nebulin (residues 6457-6528) interacted with the C-terminus of archvillin (residues 1419-1687). Archvillin is a membrane skeletal protein that localizes to costameres, specialized adhesion sites in muscle. The binding sites between nebulin and archvillin were characterized using the yeast two-hybrid system, in vitro pull-down assays, and colocalization experiments in COS-7 cells. Our data suggest a model in which archvillin attaches directly to the Z-line through an interaction with the nebulin C-terminus. The interaction between nebulin and archvillin may provide a direct link between the sarcolemma and myofibrillar Z-lines.
authors.affiliation: Department of Biology, Inje University, Gimhae 621-749, Republic of Korea.
authors.forename: Min-A
authors.initials: MA
authors.lastname: Lee
authors.forename: Young Mi
authors.initials: YM
authors.lastname: Joo
authors.forename: Yeong Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Hyun Suk
authors.initials: HS
authors.lastname: Kim
authors.forename: Ji-Hee
authors.initials: JH
authors.lastname: Kim
authors.forename: Jae-Kyong
authors.initials: JK
authors.lastname: Choi
authors.forename: Seung-Ju
authors.initials: SJ
authors.lastname: Ahn
authors.forename: Byung-In
authors.initials: BI
authors.lastname: Min
authors.forename: Chong-Rak
authors.initials: CR
authors.lastname: Kim
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism*; D018482:Muscle, Skeletal / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D012508:Sarcolemma / Q000378:metabolism*; D020798:Two-Hybrid System Techniques
chemical_list: D008565:Membrane Proteins; D008840:Microfilament Proteins; D009124:Muscle Proteins; C109413:SVIL protein, human; C035620:nebulin