title: Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage.
abstract: Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18(+/+) mice and CD18(-/-) -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitrotyrosine immunoreactivity in brains of CD18-knockout mice.
authors.affiliation: Department of Physiology and Pharmacology, Loma Linda University, Loma Linda, CA 92354, USA.
authors.forename: Elena
authors.initials: E
authors.lastname: Titova
authors.forename: Robert P
authors.initials: RP
authors.lastname: Ostrowski
authors.forename: Christopher G
authors.initials: CG
authors.lastname: Kevil
authors.forename: Wenni
authors.initials: W
authors.lastname: Tong
authors.forename: Hugo
authors.initials: H
authors.lastname: Rojas
authors.forename: Lawrence C
authors.initials: LC
authors.lastname: Sowers
authors.forename: John H
authors.initials: JH
authors.lastname: Zhang
authors.forename: Jiping
authors.initials: J
authors.lastname: Tang
mesh_terms: D000818:Animals; D001929:Brain Edema / Q000209:etiology / Q000378:metabolism / Q000473:pathology*; D018821:CD18 Antigens / Q000235:genetics / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D009195:Peroxidase / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology*; D014443:Tyrosine / Q000031:analogs & derivatives / Q000378:metabolism
chemical_list: D018821:CD18 Antigens; C002744:3-nitrotyrosine; D014443:Tyrosine; D009195:Peroxidase