title: Rapid purification of DesPro(2)-Val15-Leu17-aprotinin from the culture broth of a recombinant Saccharomyces cerevisiae.
abstract: A rapid two-step procedure has been developed for the purification of Despro(2)-Val15-Leu17-aprotinin from the culture supernatant of a recombinant yeast by affinity and ion-exchange chromatography. DesPro(2)-Val15-Leu17-aprotinin was purified to homogeneity, as demonstrated by dodecylsulfate gel electrophoresis and analysis of the N-terminal amino acid sequence.
authors.affiliation: Institut für Enzymtechnologie der Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, Jülich, Postfach, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Barthel
authors.forename: M R
authors.initials: MR
authors.lastname: Kula