title: Microcalorimetric studies of the action on four organic acids in Radix isatidis on the growth of microorganisms.
abstract: The actions of four organic acids in Radix isatidis, a traditional Chinese medicinal (TCM) herb, on Escherichia coli, Staphylococcus aureus and Shigella dysenteriae growth were investigated by microcalorimetry. The four organic acids were syringic acid, 2-amino-benzoic acid, salicylic acid and benzoic acid. The power-time curves of Escherichia coli, Staphylococcus aureus and Shigella dysenteriae growth with and without organic acids were acquired, meanwhile the extent and duration of inhibitory effects on the metabolism were evaluated by growth rate constants (k1, k2), maximum heat-output[0] power (P(m)) and peak time (t(m)). The inhibitory activity varied with different drugs. The sequences of anti-microbial activity of the four organic acids on Escherichia coli, Staphylococcus aureus and Shigella dysenteriae were all: syringic acid > 2-amino-benzoic acid > salicylic acid > benzoic acid. And benzoic acid promoted the growth of Staphylococcus aureus and Shigella dysenteriae. This study provides a basis for the further study on Radix Isatidis.
authors.affiliation: LA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Kong
authors.forename: Yanling
authors.initials: Y
authors.lastname: Zhao
authors.forename: Limei
authors.initials: L
authors.lastname: Shan
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Xiao
authors.forename: Weiying
authors.initials: W
authors.lastname: Guo
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification* / Q000494:pharmacology; D019817:Benzoic Acid / Q000302:isolation & purification* / Q000494:pharmacology; D002151:Calorimetry / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D020156:Salicylic Acid / Q000302:isolation & purification / Q000494:pharmacology; D012762:Shigella dysenteriae / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D004365:Drugs, Chinese Herbal; D005707:Gallic Acid; D019817:Benzoic Acid; C001945:syringic acid; D020156:Salicylic Acid