title: Immunohistochemical analysis of interleukin-1 alpha, its type I receptor and antagonist in keratocystic odontogenic tumors.
abstract: BACKGROUND
  Interleukin-1 alpha (IL-1 alpha) is thought to play a crucial role in the growth of keratocystic odontogenic tumors (KCOTs) in the jaw. The function of IL-1 alpha is regulated by the local levels of IL-1 alpha, its receptor and receptor antagonist (IL-1Ra) in tissues. In this study, the expression of these proteins was investigated both before and after marsupialization in KCOTs.
  
  
  METHODS
  The expression of IL-1 alpha, IL-1 receptor type I (IL-1RI) and IL-1Ra was detected immunohistochemically in 10 specimens of KCOTs.
  
  
  RESULTS
  IL-1 alpha was intensively expressed throughout the epithelium in all cases, while mild expression of IL-1 alpha was detected in the subepithelial layer endothelial cells and fibroblasts. Mild or intensive immunoreactivity for IL-1RI was also observed in the epithelial cells in all cases, and in the endothelial cells and fibroblasts in five cases respectively. The expression of IL-1Ra was detected in the epithelial cells in five cases, and in the endothelial cells and fibroblasts in three cases. After marsupialization, the immunoreactivity for IL-1 alpha and IL-1RI in the epithelial cells decreased, while the immunoreactivity for IL-1Ra in the epithelial cells increased. However, the immunoreactivity for IL-1RI and IL-1Ra in endothelial cells and fibroblasts did not change significantly.
  
  
  CONCLUSIONS
  The effects of IL-1 alpha on the epithelial cells might be downregulated after marsupialization by changing the expression levels of IL-1 alpha, IL-1RI and IL-1Ra in the epithelium of KCOTs.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Suyama
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Kubota
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Ninomiya
authors.forename: Kanemitsu
authors.initials: K
authors.lastname: Shirasuna
mesh_terms: D000328:Adult; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007150:Immunohistochemistry; D053590:Interleukin 1 Receptor Antagonist Protein / Q000378:metabolism*; D053582:Interleukin-1alpha / Q000378:metabolism*; D007573:Jaw Neoplasms / Q000150:complications / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009807:Odontogenic Cysts / Q000150:complications / Q000378:metabolism*; D009808:Odontogenic Tumors / Q000150:complications / Q000378:metabolism*; D017472:Receptors, Interleukin-1 / Q000378:metabolism*
chemical_list: D053590:Interleukin 1 Receptor Antagonist Protein; D053582:Interleukin-1alpha; D017472:Receptors, Interleukin-1