Injection of biogenic amines modulates osmoregulation of Litopenaeus vannamei: response of hemolymph osmotic pressure, ion concentration and osmolality effectors.
In this paper, we compared systematically the temporal and dose response relationship and physiological significance among biogenic amines injection, changes of ion concentration, FAA concentrations and composition and protein in context of osmoregulatory ability in marine euryhaline shrimp: Litopenaeus vannamei. The dopamine (DA) and 5-hydroxytryptamine (5-HT) injection all had transient effects on hemolymph osmolality, ion concentrations but which occurred in different time and were dose-dependent. The highest concentrations of FAAs in hemolymph of L. vannamei were alanine, glycine, argnine, proline, lysine which were considered to be specific osmotic effectors. Contrary to the reduction of hemocyanin, injection of DA 10(-6) mol shrimp(-1) and 5-HT 10(-6) mol shrimp(-1) induced notable protein increase respectively, which led to the rapid reduction of hemocyanin/protein ratio in range of 63.2% to 78.3%. The increase of hemolymph FAAs might come from the new amino acid synthesis or degradation of muscle protein to FAAs or denovo synthesis of FAAs. Our study showed that dopamine plays an important role in neurotransmission and causes osmoregulation response modulation and 5-HT has different activation mechanism on osmoregulation.
Liu
Hong-yu
HY
The Key Laboratory of Mariculture of Chinese, Ministry of Education, Ocean University of China, Qingdao 266003, China.
Pan
Lu-qing
LQ
Zheng
De-bin
DB
D000596:Amino Acids / Q000096:biosynthesis; D000818:Animals; D001679:Biogenic Amines / Q000008:administration & dosage / Q000494:pharmacology; D004298:Dopamine / Q000008:administration & dosage / Q000494:pharmacology; D004305:Dose-Response Relationship, Drug; D006433:Hemocyanins / Q000378:metabolism; D006458:Hemolymph / Q000187:drug effects* / Q000502:physiology; D009124:Muscle Proteins / Q000378:metabolism; D018377:Neurotransmitter Agents / Q000378:metabolism; D009994:Osmolar Concentration; D009997:Osmotic Pressure / Q000187:drug effects; D033561:Penaeidae / Q000187:drug effects* / Q000502:physiology; D012701:Serotonin / Q000008:administration & dosage / Q000494:pharmacology; D013997:Time Factors; D014882:Water-Electrolyte Balance / Q000187:drug effects* / Q000502:physiology
D000596:Amino Acids; D001679:Biogenic Amines; D009124:Muscle Proteins; D018377:Neurotransmitter Agents; D012701:Serotonin; D006433:Hemocyanins; D004298:Dopamine