Reinvestigation of the O-antigens of Yersinia pseudotuberculosis: revision of the O2c and confirmation of the O3 antigen structures.
Structures of the O-antigens of Yersinia pseudotuberculosis O2c and O3 were reinvestigated by NMR spectroscopy, including 2D (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC, and HMBC experiments. The following revised structure of the O2c tetrasaccharide repeating unit was established, which differs from the structure proposed earlier in the glycosylation pattern of the mannose residue at the branching point: [carbohydrate structure: see text] where Abe stands for 3,6-dideoxy-d-xylo-hexose. The structure of the Y. pseudotuberculosis O3 antigen reported earlier was confirmed.
Kondakova
Anna N
AN
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia. annakond@gmail.com
Bystrova
Olga V
OV
Shaikhutdinova
Rima Z
RZ
Ivanov
Sergey A
SA
Dentovskaya
Svetlana V
SV
Shashkov
Alexander S
AS
Knirel
Yuriy A
YA
Anisimov
Andrey P
AP
D002240:Carbohydrate Sequence; D009682:Magnetic Resonance Spectroscopy; D008969:Molecular Sequence Data; D019081:O Antigens / Q000737:chemistry*; D015011:Yersinia pseudotuberculosis / Q000737:chemistry*
D019081:O Antigens