title: Matrix-assisted laser desorption ionisation-time-of of-flight mass spectrometry of intact cells allows rapid identification of Burkholderia cepacia complex.
abstract: The present study examined the potential of intact-cell matrix-assisted laser desorption ionisation-time-of-flight mass spectrometry (MALDI-TOF MS) for a rapid identification of Burkholderia cepacia complex (Bcc) bacteria using an Applied Biosystems 4700 Proteomics Analyser. Two software packages were used to analyse mass profiles based on densitometric curves and peak positions. The 75 strains examined, represented the nine established Bcc species and some commonly misidentified species, closely related or biochemically similar to Bcc and relevant in the context of cystic fibrosis microbiology. All Bcc strains clustered together, separated from non-Bcc strains. Within Bcc, most Bcc strains grouped in species specific clusters, except for Burkholderia anthina and Burkholderia pyrrocinia strains which constituted a single cluster. The present study demonstrates that MALDI-TOF MS is a powerful approach for the rapid identification of Bcc bacteria.
authors.affiliation: Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
authors.forename: Elke
authors.initials: E
authors.lastname: Vanlaere
authors.forename: Kjell
authors.initials: K
authors.lastname: Sergeant
authors.forename: Peter
authors.initials: P
authors.lastname: Dawyndt
authors.forename: Wibke
authors.initials: W
authors.lastname: Kallow
authors.forename: Marcel
authors.initials: M
authors.lastname: Erhard
authors.forename: Helen
authors.initials: H
authors.lastname: Sutton
authors.forename: Diane
authors.initials: D
authors.lastname: Dare
authors.forename: Bart
authors.initials: B
authors.lastname: Devreese
authors.forename: Bart
authors.initials: B
authors.lastname: Samyn
authors.forename: Peter
authors.initials: P
authors.lastname: Vandamme
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D019121:Burkholderia Infections / Q000382:microbiology; D042602:Burkholderia cepacia complex / Q000145:classification* / Q000166:cytology* / Q000235:genetics / Q000302:isolation & purification; D003550:Cystic Fibrosis / Q000382:microbiology; D004783:Environmental Microbiology; D006801:Humans; D040901:Proteomics / Q000295:instrumentation; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation / Q000379:methods*; D013997:Time Factors