title: Biochemical characterization of a glycosyltransferase homolog from an oral pathogen Fusobacterium nucleatum as a human glycan-modifying enzyme.
abstract: Bacterial glycosyltransferases have drawn growing attention as economical enzymes for oligosaccharide synthesis, with their easy expression and relatively broad substrate specificity. Here, we characterized a glycosyltransferase homolog (Fnu_GT) from a human oral pathogen, Fusobacterium nucleatum. Bioinformatic analysis showed that Fnu_GT belongs to the glycosyltransferases family II. The recombinant Fnu_GT (rFnu_GT) expressed in Escherichia coli displayed the highest glycosylation activity when UDP-galactose (Gal) was used as a donor nucleotide-sugar with heptose or Nacetylglucosamine (GlcNAc) as an acceptor sugar. Interestingly, rFnu_GT transferred the galactose moiety of UDP-Gal to a nonreducing terminal GlcNAc attached to the trimannosyl core glycan, indicating its potential as an enzyme for humantype N-glycan synthesis.
authors.affiliation: Omics and Integration Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 305-806, Korea.
authors.forename: Seonghun
authors.initials: S
authors.lastname: Kim
authors.forename: Doo-Byoung
authors.initials: DB
authors.lastname: Oh
authors.forename: Ohsuk
authors.initials: O
authors.lastname: Kwon
authors.forename: Jae Gap
authors.initials: JG
authors.lastname: Jung
authors.forename: Yun Mi
authors.initials: YM
authors.lastname: Lee
authors.forename: Kisung
authors.initials: K
authors.lastname: Ko
authors.forename: Jeong Heon
authors.initials: JH
authors.lastname: Ko
authors.forename: Hyun Ah
authors.initials: HA
authors.lastname: Kang
mesh_terms: D000595:Amino Acid Sequence; D001419:Bacteria / Q000145:classification / Q000201:enzymology / Q000235:genetics; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D003001:Cloning, Molecular; D004795:Enzyme Stability; D016967:Fusobacterium nucleatum / Q000737:chemistry / Q000201:enzymology* / Q000235:genetics; D015870:Gene Expression; D016695:Glycosyltransferases / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D007700:Kinetics; D008969:Molecular Sequence Data; D009055:Mouth / Q000382:microbiology*; D010802:Phylogeny; D011134:Polysaccharides / Q000378:metabolism*; D017386:Sequence Homology, Amino Acid; D013379:Substrate Specificity
chemical_list: D001426:Bacterial Proteins; D011134:Polysaccharides; D016695:Glycosyltransferases