title: Rapid identification of triterpenoid saponins in the roots of Codonopsis lanceolata by liquid chromatography-mass spectrometry.
abstract: Liquid chromatography coupled with sequential mass spectrometry (LC-MS(n)) has been used to identify 3,28-bidesmosidic triterpenoid saponins, lancemaside A (1), foetidissimoside A (2), aster saponin Hb (3), lancemaside E (4), lancemaside B (5), lancemaside F (6), lancemaside G (7), lancemaside C (8), and lancemaside D (9) in the roots of Codonopsis lanceolata. Structural information about both the aglycone and the sugar moiety at the C-3 position of saponins was obtained in the negative-ion mode. On the other hand, positive-ion spectra mainly provide structural information about the sugar chains of saponins, especially the oligosaccharide moiety at the C-28 position. During subsequent fragmentation of the product ions derived from the oligosaccharide moiety at the C-28 position, fragments produced by sequential loss of a monosaccharide unit were observed. Furthermore, the structural features of two unknown saponins in the roots of C. lanceolata were assigned on the basis of the fragmentation patterns of the known saponins. These studies demonstrate that LC-MS(n) analysis has great potential for the identification and characterization of triterpenoid saponins in plant extracts.
authors.affiliation: Healthcare Research Institute, Wakunaga Pharmaceutical Co., Ltd, 1624 Shimokotachi, Kodacho, Akitakata, Hiroshima, 739-1195, Japan. ichikawa_m@wakunaga.co.jp
authors.forename: Makoto
authors.initials: M
authors.lastname: Ichikawa
authors.forename: Sanae
authors.initials: S
authors.lastname: Ohta
authors.forename: Noriko
authors.initials: N
authors.lastname: Komoto
authors.forename: Mitsuyasu
authors.initials: M
authors.lastname: Ushijima
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Kodera
authors.forename: Minoru
authors.initials: M
authors.lastname: Hayama
authors.forename: Osamu
authors.initials: O
authors.lastname: Shirota
authors.forename: Setsuko
authors.initials: S
authors.lastname: Sekita
authors.forename: Masanori
authors.initials: M
authors.lastname: Kuroyanagi
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D029745:Codonopsis / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods; D018517:Plant Roots; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification*; D014315:Triterpenes / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D012503:Saponins; D014315:Triterpenes