title: The use of contrast-enhanced US in renal transplant: first results and potential clinical benefit.
abstract: OBJECTIVE
  Little use has been made so far of the intrinsic advantages of ultrasound (US) for quantifying tissue perfusion of parenchymal organs, that is, its high spatial and temporal resolution and immediate availability in any clinical situation. Since acute rejection of a kidney graft primarily involves the sub-capsular capillaries, early and detailed evaluation of blood flow in this area is highly desirable. Using a clinically established US contrast medium (USCM) of the second generation and improved US technology, we performed a study to investigate whether it is possible to adequately diagnose rejection after kidney transplant by evaluating the arterial inflow of an echo enhancer.
  
  
  METHODS
  A total of 32 patients underwent US examination with an echo enhancer (1.6 ml SonoVue, Bracco-Altana) 5 to 7 days after kidney transplantation. The examinations were performed using the Aplio US system (Toshiba) with a 3.5-MHz transducer and micro flow imaging (MI 0.1). Contrast medium inflow was determined in the renal artery and the renal cortex using Windows-based, time-intensity curve (TIC) software. The temporal difference in contrast medium inflow between the two vascular territories was determined (Dt = time baseline renal cortex--time baseline renal artery). Patients with primary graft failure (no function until day 3) were excluded (number of patients, n=2). In patients with large peri-renal hematoma (n=6), the effect of US on perfusion was determined and the results in these cases (hematoma group) were compared with those in the remaining patients.
  
  
  RESULTS
  Seventeen patients had uneventful clinical course (resistance index (RI) on day 7: 0.75+/-0.11). In this group US demonstrated a uniform inflow of the CM. The calculated slopes were comparable with those of the interlobar artery and renal cortex (no rejection group). Seven patients showed histologically confirmed acute rejection on days 5 to 7 after transplantation (rejection group). The RI in this group was at the upper limit on day 7 (0.77+/-0.08). The temporal difference in CM arrival between the two vascular territories was greater in the rejection group (2.27+/-0.73 s) compared with the normal group (0.97+/-0.62 s, p<0.05, p=significance). The difference was also increased in the hematoma group (1.5+/-1.3 s, p>0.05). The size of the hematoma correlated with the extent to which USCM inflow was altered. In only two cases, the USCM examination demonstrated a perfusion defect.
  
  
  CONCLUSIONS
  The use of echo enhancers has potential to diagnose acute kidney graft rejection in its early stages. US not only identifies kidney perfusion defects but also provides information on the effect of a large peri-renal hematoma.
authors.affiliation: Department of Radiology, Universitätsmedizin Berlin, Campus Charité Mitte, Schumannstrasse 20/21, 10098 Berlin, Germany. thom.fischer@charite.de
authors.forename: Thomas
authors.initials: T
authors.lastname: Fischer
authors.forename: Jan
authors.initials: J
authors.lastname: Dieckhöfer
authors.forename: Matthias
authors.initials: M
authors.lastname: Mühler
authors.forename: Alexander
authors.initials: A
authors.lastname: Lembcke
authors.forename: Stanislao
authors.initials: S
authors.lastname: Morgera
authors.forename: Klemens
authors.initials: K
authors.lastname: Budde
authors.forename: Hans-Helmuth
authors.initials: HH
authors.lastname: Neumayer
authors.forename: Volko
authors.initials: V
authors.lastname: Ebeling
authors.forename: Anke
authors.initials: A
authors.lastname: Thomas
authors.forename: Serjei
authors.initials: S
authors.lastname: Filimonow
mesh_terms: D000328:Adult; D000368:Aged; D003287:Contrast Media; D005260:Female; D006084:Graft Rejection / Q000000981:diagnostic imaging*; D006801:Humans; D007668:Kidney / Q000098:blood supply* / Q000000981:diagnostic imaging; D016030:Kidney Transplantation / Q000000981:diagnostic imaging*; D008297:Male; D008875:Middle Aged; D010743:Phospholipids; D018709:Statistics, Nonparametric; D013459:Sulfur Hexafluoride; D016896:Treatment Outcome; D014463:Ultrasonography
chemical_list: D003287:Contrast Media; D010743:Phospholipids; C420843:contrast agent BR1; D013459:Sulfur Hexafluoride