title: Restricted Semliki Forest virus replication in perforin and Fas-ligand double-deficient mice.
abstract: Previously, we have shown that mice defective in granule exocytosis and/or Fas.L/Fas-mediated cytolytic pathways are significantly more resistant to alphavirus, Semliki Forest virus (SFV), infection compared with wild-type mice. Here, we evaluated SFV replication in different tissues of mice defective in both cytolytic pathways (perf(-/-)xgld) relative to that in wild-type counterparts and found that viral replication in perf(-/-)xgld mice is remarkably restricted. Although the mechanism responsible for this observation is yet to be established, the lower virus titres found in these mice indicate that the role of cytolytic effector molecules in antiviral immunity needs to be re-evaluated.
authors.affiliation: Division of Immunology and Genetics, The John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Alsharifi
authors.affiliation: Division of Immunology and Genetics, The John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Mario
authors.initials: M
authors.lastname: Lobigs
authors.affiliation: Division of Immunology and Genetics, The John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Jayaram
authors.initials: J
authors.lastname: Bettadapura
authors.affiliation: Division of Immunology and Genetics, The John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Aulikki
authors.initials: A
authors.lastname: Koskinen
authors.affiliation: Division of Immunology and Genetics, The John Curtin School of Medical Research, Australian National University, Canberra, ACT 0200, Australia.
authors.forename: Arno
authors.initials: A
authors.lastname: Müllbacher
mesh_terms: D018354:Alphavirus Infections / Q000276:immunology* / Q000821:virology; D000818:Animals; D053222:Fas Ligand Protein / Q000172:deficiency* / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009928:Organ Specificity; D054353:Perforin / Q000172:deficiency* / Q000235:genetics; D012672:Semliki forest virus / Q000472:pathogenicity* / Q000502:physiology*; D014779:Virus Replication
chemical_list: D053222:Fas Ligand Protein; D054353:Perforin