title: The effects of duration of CO2 pneumoperitoneum on colonic anastomosis.
abstract: The aim of this study is to evaluate the effects of duration of carbon dioxide (CO(2)) pneumoperitoneum on experimental colonic anastomosis. Forty-eight male Sprague-Dawley rats were used. The rats were divided into three groups. The rats in group 1 (n = 16) underwent laparotomy and colonic anastomosis without pneumoperitoneum. The rats in group 2 (n = 16) and group 3 (n = 16) were subjected to 2 and 4 hours of 12 mm Hg pneumoperitoneum, respectively, before laparotomy and colonic anastomosis. Half of the rats were sacrified on the third postoperative day; and the other half, on the seventh postoperative day. A colonic segment including anastomosis site was resected for histopathologic and biochemical evaluation. On day 3, hydroxyproline levels of the three groups were similar. The edema score of group 2 was significantly higher than that of group 1, and the necrosis score was higher in group 2 than in group 3. The scores of the other histopathologic parameters were similar. On day 7, group 3 showed significantly higher hydroxyproline levels than group 1, and group 1 showed a higher necrosis score than group 3. In conclusion, CO(2) pneumoperitoneum of 12 mm Hg for 2 and 4 hours did not result in impaired healing of experimental colonic anastomosis.
authors.affiliation: Department of Gastrointestinal Surgery, Turkiye Yuksek Ihtisas Teaching and Research Hospital, Ankara, Turkey. ilterozer@yahoo.co
authors.forename: Ilter
authors.initials: I
authors.lastname: Ozer
authors.forename: Murat
authors.initials: M
authors.lastname: Ulas
authors.forename: Metin
authors.initials: M
authors.lastname: Ercan
authors.forename: Yusuf B
authors.initials: YB
authors.lastname: Ozogul
authors.forename: Neslihan
authors.initials: N
authors.lastname: Zengin
authors.forename: E Birol
authors.initials: EB
authors.lastname: Bostanci
authors.forename: Ummuhani
authors.initials: U
authors.lastname: Ozel
authors.forename: Ayse
authors.initials: A
authors.lastname: Bilgihan
authors.forename: Musa
authors.initials: M
authors.lastname: Akoglu
mesh_terms: D000714:Anastomosis, Surgical / Q000379:methods*; D000818:Animals; D002245:Carbon Dioxide; D003106:Colon / Q000378:metabolism / Q000473:pathology / Q000601:surgery*; D004487:Edema / Q000473:pathology; D006909:Hydroxyproline / Q000378:metabolism; D008297:Male; D009336:Necrosis; D011028:Pneumoperitoneum, Artificial; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors; D014945:Wound Healing
chemical_list: D002245:Carbon Dioxide; D006909:Hydroxyproline