In vivo osteoprogenitor potency of human stromal cells from different tissues does not correlate with expression of POU5F1 or its pseudogenes.
Expression of "stemness" markers is widely used as a predictor of stem cell properties of mesenchymal stem cells (MSC). Here, we show that bone marrow-derived (BM)-MSC show stem cell-like behavior in vivo; that is, they form ossicles with formation of bone, formation of adipocytes, and establishment of the murine hematopoietic microenvironment. Multipotent umbilical vein-derived stromal cells (UVSC), on the other hand, do not form bone, nor do they give rise to adipocytes in vivo. Despite these differences in stem-cell-like behavior, BM-MSC and UVSC express the two transcripts variants of POU5F1 at a similar level. Also, we found that in BM-MSC and UVSC, POU5F1 is detectable. However, more than 89% of the POU5F1 transcripts correspond to the POU5F1P1, -P3, or -P4 pseudogene. Despite low-level expression of POU5F1, we were unable to precipitate POU5F1 protein in either BM-MSC or UVSC. These results demonstrate that MSC stemness does not correlate to expression of POU5F1 transcripts or its pseudogenes.
Kaltz
Nikolas
N
Third Department of Internal Medicine, Klinikum rechts der Isar, Technische Universität München, München, Germany.
Funari
Alessia
A
Hippauf
Sandra
S
Delorme
Bruno
B
Noël
Danièle
D
Riminucci
Mara
M
Jacobs
Volker R
VR
Häupl
Thomas
T
Jorgensen
Christian
C
Charbord
Pierre
P
Peschel
Christian
C
Bianco
Paolo
P
Oostendorp
Robert A J
RA
D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000943:Antigens, Differentiation / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D019070:Cell Lineage; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000096:biosynthesis* / Q000235:genetics; D010012:Osteogenesis; D011544:Pseudogenes; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism; D014471:Umbilical Veins / Q000166:cytology
D000943:Antigens, Differentiation; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human