Integration of lambda b2 in Escherichia coli K12-B hybrid.
The gal-bio region was transduced from Escherichia coli B to K12 and lysogenized with lambda b2. By means of P1 transduction mapping, the lambda b2 prophage was shown to be inserted between gal and bio and to be circularly permuted in the same way as lambda b2+ in K12. Increased frequency of recombinations within the prophage was observed when the viral genes were mapped with respect to bio instead of to gal. The results are interpreted to indicate the existence of a region in lambda situated close to the b2 region and homologous with a lambda attachment site of E. coli B but not with that of E. coli K12.
Zavada
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International Laboratory of Genetics and Biophysics, Naples, Italy.
Calef
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