title: Cultivation of an L-lactate dehydrogenase mutant of Bacillus stearothermophilus in continuous culture with cell recycle.
abstract: Continuous fermentation with cell recycle proved very effective in increasing the ethanol volumetric productivity of the thermophilic facultative anaerobe, Bacillus stearothermophilus strain LLD-15, on sucrose at 70 degrees C. When complete cell recycle was used, cell viability decreased after a few residence times and sucrose consumption was reduced. Operation using a constant bleed rate resulted in greater stability and higher ethanol volumetric productivities. A mathematical model based on maintenance energy requirements provided an adequate description of the system.
authors.affiliation: Centre for Biotechnology, Imperial College of Science, Technology and Medicine, London, United Kingdom.
authors.forename: R S
authors.initials: RS
authors.lastname: Martin
authors.forename: D
authors.initials: D
authors.lastname: Bushell
authors.forename: D J
authors.initials: DJ
authors.lastname: Leak
authors.forename: B S
authors.initials: BS
authors.lastname: Hartley