[High-level production of alkaline polygalacturonate lyase in recombinant Pichia pastoris].
In order to increase the production of alkaline polygalacturonate lyase (PGL) by recombinant Pichia pastoris GS115, the effect of cell and methanol concentration on the PGL production was carefully investigated by single factor experiment. The optimum conditions were listed as follows: the cell concentration 122 g/L, the methanol concentration 20 g/L, and the ratio of methanol and cell concentration 0.16-0.20 g/g (methanol/cell). With the glycerol and methanol feeding strategies, the ratio of methanol and cell concentration could be controlled at the range of 0.171 to 0.195 g/g. And the highest PGL activity (430 u/mL) and highest PGL productivity (4.34 u/mL/h) were achieved.
Wang
Yun
Y
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Hua
Zhaozhe
Z
Liu
Liming
L
Zhang
Zhaohui
Z
Du
Guocheng
G
Chen
Jian
J
D000468:Alkalies / Q000378:metabolism; D005285:Fermentation; D005822:Genetic Vectors; D000432:Methanol / Q000737:chemistry; D010843:Pichia / Q000201:enzymology* / Q000235:genetics; D011133:Polysaccharide-Lyases / Q000096:biosynthesis* / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis* / Q000235:genetics; D011995:Recombination, Genetic
D000468:Alkalies; D011994:Recombinant Proteins; D011133:Polysaccharide-Lyases; C023911:pectate lyase; D000432:Methanol