Forssman antigen content of guinea pig hepatomas induced by diethylnitrosamine: a quantitative approach to the search for tumor-specific antibodies.
The Forssman antigen content of diethylnitrosamine-induced guinea pig hepatomas was found to be greater than that of either autogenous or allogeneic guinea pig liver. The autogenous normal liver was obtained from guinea pigs before tumor induction, and comparison of normal and neoplastic tissues was based on the capacity of these tissues to inhibit (absorb) the hemolytic activity of rabbit antitumor serum. A method is presented for distinguishing quantitative from qualitative antigenic differences in the search for tumor-specific antigens. The results of these experiments indicate that, in the absence of strict quantitation, quantitative differences may be mistaken for qualitative differences.
Crisler
C
C
Immunochemistry Section, Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.
Rapp
H J
HJ
Weintraub
R M
RM
Borsos
T
T
D000818:Animals; D000912:Antibodies, Neoplasm / Q000032:analysis*; D000951:Antigens, Neoplasm / Q000032:analysis*; D002273:Carcinogens; D004052:Diethylnitrosamine; D005577:Forssman Antigen / Q000032:analysis*; D006168:Guinea Pigs; D008114:Liver Neoplasms, Experimental / Q000139:chemically induced / Q000276:immunology*
D000912:Antibodies, Neoplasm; D000951:Antigens, Neoplasm; D002273:Carcinogens; D004052:Diethylnitrosamine; D005577:Forssman Antigen