Monocyte-derived dendritic cells as antigen-presenting cells in T-cell proliferation and cytokine production.
Dendritic cells (DC) are widely considered to be the major antigen-presenting cell (APC) type in immune responses. These cells are obtained from adherent cells or are purified CD14(+) monocytes from peripheral blood mononuclear cells (PBMC) by in vitro stimulation with granulocyte, macrophage-colony-stimulating factor (GM-CSF) plus interleukin (IL)-4. They express high levels of MHC class II and costimulatory molecules, internalize Ag rapidly via Fc receptors and mannose receptors, and, by macropinocytosis, produce large amounts of IL-12 on CD40 ligation, and are potent in presenting soluble Ag and in stimulating allogeneic mixed-leukocyte reactions. To study primary T-cell responses and cytokine production in allergy patients, we have developed an in vitro system by using highly purified T cells as responder cells and monocyte-derived DC (MDC) as the APC. MDC provide a convenient and potent APC source for T-cell response studies.
Sung
Sun-sang J
SS
Division of Rheumatology, University of Virginia Health Sciences Center, Charlottesville, VA.
D017951:Antigen Presentation; D049109:Cell Proliferation; D002469:Cell Separation; D016207:Cytokines / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000276:immunology; D008213:Lymphocyte Activation; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology / Q000276:immunology*
D016207:Cytokines; D018950:Lipopolysaccharide Receptors