Kinetic properties and inhibition of the dimeric dUTPase-dUDPase from Campylobacter jejuni.
The enzyme deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase) catalyses the hydrolysis of dUTP to dUMP and PPi thus controlling the incorporation of uracil into DNA genomes. In Campylobacter jejuni dUTPase exhibits structural properties of dimeric proteins characteristic of protozoa of the Kinetoplastidae family. In the present study we perform a kinetic analysis of Campylobacter dUTPase using the continuous spectrophotometric method and show that the enzyme is highly specific for deoxyuridine nucleotides. The Michaelis-Menten constant for dUTP was 0.66 microM while the k(cat) was 12.3 s(- 1). dUDP was also efficiently hydrolysed although the specificity constant, k(cat)/K(m), was five fold lower than for dUTP. The reaction product and the non hydrolysable analogue alpha,beta imido dUDP are potent inhibitors of the enzyme while several analogues of dUMP with substituents at the 3'- and 5'-positions active against trimeric dUTPases, show poor inhibitory activity. Apparent structural and kinetic differences with other eukaryotic dUTPases suggest that the present enzyme might be exploited as a target for new drugs against campylobacteriosis.
Musso-Buendía
Juan A
JA
Instituto de Parastiologia y Biomedicina "Lopez-Neyra". Consejo Superior de Investigaciones Cientificas, Armilla, Granada, Spain.
Vidal
Antonio E
AE
Kasinthan
Ganasan
G
Nguyen
Corinne
C
Carrero-Lérida
Juana
J
Ruiz-Pérez
Luis M
LM
Wilson
Keith
K
Johansson
Nils Gunnar
NG
Gilbert
Ian H
IH
González-Pacanowska
Dolores
D
D001426:Bacterial Proteins; D016123:Campylobacter jejuni / Q000201:enzymology*; D019281:Dimerization; D006868:Hydrolysis; D007700:Kinetics; D011755:Pyrophosphatases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D013379:Substrate Specificity; D014544:Uridine Triphosphate / Q000378:metabolism
D001426:Bacterial Proteins; D011755:Pyrophosphatases; C023273:uridine diphosphatase; C020020:dUTP pyrophosphatase; D014544:Uridine Triphosphate