Differentiation-associated alteration in gene expression of importins and exportins in human leukemia HL-60 cells.
Employing the DNA microarray technique, we previously reported the alteration in gene expression of nucleocytoplasmic transport factors, importins and exportins, induced by 1,25-dihydroxyvitamin D3 (DVD) in human leukemia HL-60 cells. Here, we used the quantitative reverse transcription-polymerase chain reaction method to confirm such previous findings, and compared them with those from the cells treated with all-trans-retinoic acid (ATRA). The results indicated that the gene expression of the transport factors examined was mostly down-regulated following differentiation induced by DVD and ATRA, but importin alpha5 gene expression was up-regulated in either case. The differences were found in the gene expression of importin alpha3 and exportin 6 between the cells after treatments with DVD and ATRA. These variations may be related to the difference between HL-60 cell lineages differentiating into monocytes/macrophages and granulocytes. The present findings provide further evidence to support the important roles of importins and exportins in cell differentiation.
Suzuki
Takuji
T
Graduate School of Nutritional and Environmental Sciences and Global COE, University of Shizuoka, Shizuoka, Japan.
Ishigami
Yoko
Y
Okada
Norihisa
N
Kaneko
Akihiro
A
Fukutomi
Ryuuta
R
Isemura
Mamoru
M
D001483:Base Sequence; D002117:Calcitriol / Q000494:pharmacology; D017931:DNA Primers; D015870:Gene Expression; D018922:HL-60 Cells; D006801:Humans; D028884:Karyopherins / Q000378:metabolism*; D015473:Leukemia, Promyelocytic, Acute / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D014212:Tretinoin / Q000494:pharmacology
D017931:DNA Primers; D028884:Karyopherins; D014212:Tretinoin; D002117:Calcitriol