New diterpenoid alkaloids from Aconitum heterophyllum Wall: Selective butyrylcholinestrase inhibitors.
Two new diterpenoid alkaloids, heterophyllinine-A (1) and heterophyllinine-B (2), along with two known alkaloids dihydroatisine (3) and lycoctonine (4) were isolated from the roots of Aconitum heterophyllum Wall. The structure of (1) and (2), were deduced on the basis of spectral data. Compounds 1-2 inhibited acetylcholinesterase (AChE, EC 3.1.1.7) and butyrylcholinesterase (BChE, EC 3.1.1.8) enzymes in a concentration-dependent manner with percent inhibition ranging between 4.24% and 6.94 % and 79.1 % and 82.75 % for AChE and BChE, respectively indicating that compounds 1 and 2 are about thirteen times more specific to BChE than AChE.
Nisar
Muhammad
M
Institute of Chemical Sciences and Department of Pharmacy, University of Peshawar, Peshawar, Pakistan. akhund_54@yahoo.co.uk
Ahmad
Manzoor
M
Wadood
Noreen
N
Lodhi
Muhammad Arif
MA
Shaheen
Farzana
F
Choudhary
M Iqbal
MI
D000110:Acetylcholinesterase / Q000187:drug effects; D000157:Aconitine / Q000031:analogs & derivatives; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D000880:Anthraquinones; D002091:Butyrylcholinesterase / Q000187:drug effects*; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D018517:Plant Roots / Q000737:chemistry
D000470:Alkaloids; D000880:Anthraquinones; D002800:Cholinesterase Inhibitors; D004224:Diterpenes; C511570:heterophylline; C539171:heterophyllinine A; C539172:heterophyllinine B; C093992:lycoctonine; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D000157:Aconitine