title: Sarcocystis neurona: molecular characterization of enolase domain I region and a comparison to other protozoa.
abstract: Sarcocystis neurona causes protozoal myeloencephalitis and has the ability to infect a wide host range in contrast to other Sarcocystis species. In the current study, five S. neurona isolates from a variety of sources, three Sarcocystis falcatula, one Sarcocystis dasypi/S. neurona-like isolate, and one Besnoitia darlingi isolate were used to compare the enolase 2 gene segment containing the domain I region to previously sequenced enolase genes from Neospora caninum, Neospora hughesi, Toxoplasma gondii, Plasmodium falciparum, and Trypanosoma cruzi; enolase 2 segment containing domain I region is highly conserved amongst these parasites of veterinary and medical importance. Immunohistochemistry results indicates reactivity of T. gondii enolase 1 and 2 antibodies to S. neurona merozoites and metrocytes, but no reactivity of anti-enolase 1 to the S. neurona bradyzoite stage despite reactivity to T. gondii bradyzoites, suggesting expression differences between organisms.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, 601 Vernon Tharp Street, Columbus, OH 43210, USA.
authors.forename: K E
authors.initials: KE
authors.lastname: Bolten
authors.forename: A E
authors.initials: AE
authors.lastname: Marsh
authors.forename: S M
authors.initials: SM
authors.lastname: Reed
authors.forename: J P
authors.initials: JP
authors.lastname: Dubey
authors.forename: R E
authors.initials: RE
authors.lastname: Toribio
authors.forename: W J A
authors.initials: WJ
authors.lastname: Saville
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001135:Armadillos; D001483:Base Sequence; D016054:DNA, Protozoan / Q000737:chemistry; D006736:Horses; D015151:Immunoblotting / Q000662:veterinary; D007150:Immunohistochemistry / Q000662:veterinary; D051379:Mice; D018345:Mice, Knockout; D008969:Molecular Sequence Data; D009893:Opossums; D010751:Phosphopyruvate Hydratase / Q000737:chemistry / Q000235:genetics* / Q000276:immunology; D012522:Sarcocystis / Q000201:enzymology* / Q000235:genetics / Q000276:immunology; D016415:Sequence Alignment / Q000662:veterinary
chemical_list: D016054:DNA, Protozoan; D010751:Phosphopyruvate Hydratase