title: Development of rat antigen-presenting cells from pluripotent ecto-mesenchymal stem cells in vitro and in vivo.
abstract: Dendritic cells (DC) are specialized cells that capture and present antigen to T cells. Recent advances have been made in understanding their origin, heterogeneity, and the signals that induce their migration and maturation resident microglia are antigen-presenting cells (APC) involved in stimulation or reactivation of CNS-targeted T cells. Generation of DC from microglia, as demonstrated ex vivo, may support GM-CSF-driven differentiation of brain DC from local, likely, microglial progenitors. Here, we report the establishment of long-term cultures of rat ecto-mesenchymal stem cells (EMSCs) using specific supplemented media for induction. These EMSCs share some morphological characteristics and the allostimulatory capacity of classical DCs, and when transplanted into the brain using a rat glioma model survive within the cortex, and are morphologically and phenotypically similar to microglia over 7 days. Our findings related to the development and differentiation of microglial progenitors support the view that microglia are derived prenatally from mesodermal progenitors that are distinct from monocytes.
authors.affiliation: Institute of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Shaanxi, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Hu
authors.forename: Xuefeng
authors.initials: X
authors.lastname: Shen
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Zhang
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Deng
authors.forename: Yunxin
authors.initials: Y
authors.lastname: Cao
authors.forename: Zeyuan
authors.initials: Z
authors.lastname: Zhou
authors.forename: Jinwu
authors.initials: J
authors.lastname: Chen
authors.forename: Guanqun
authors.initials: G
authors.lastname: Ge
authors.forename: Kun
authors.initials: K
authors.lastname: Xuan
authors.forename: Xiang
authors.initials: X
authors.lastname: Zhang
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D003713:Dendritic Cells / Q000276:immunology* / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D005910:Glioma / Q000473:pathology; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009368:Neoplasm Transplantation; D039904:Pluripotent Stem Cells / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014184:Transplantation, Homologous
chemical_list: D003470:Culture Media