title: Treatment of phenolic wastewater in an anaerobic fixed bed reactor (AFBR) - recovery after shock loading.
abstract: An anaerobic fixed bed reactor (AFBR) was run for 550 days with a mixed microbial flora to stabilize synthetic wastewater that contained glucose and phenol as main carbon sources. The influent phenol concentration was gradually increased from 2 to 40 mmol/l within 221 days. The microbial flora was able to adapt to this high phenol concentration with an average of 94% phenol removal. Microbial adaptation at such a high phenol concentration is not reported elsewhere. The maximum phenol removal observed before the phenol shock load was 39.47 mmol/l or 3.7 g phenol/l at a hydraulic retention time (HRT) of 2.5 days and an organic loading rate (OLR) of 5.3 g/l.d which amounts to a phenol removal rate of ca. 15.8 mmol phenol/l.d. The chemical oxygen demand (COD) removal before exposing the reactor to a shock load corresponded with phenol removal. A shock load was induced in the reactor by increasing the phenol concentration from 40 to 50 mmol/l in the influent. The maximum phenol removal rate observed after shock load was 18 mmol/l.d at 5.7 g COD/l.d. But this was not a stable rate and a consistent drop in COD and phenol removal was observed for 1 week, followed by a sharp decline and production of fatty acids. Recovery of the reactor was possible only when no feed was provided to the reactor for 1 month and the phenol concentration was increased gradually. When glucose was omitted from the influent, unknown intermediates of anaerobic phenol metabolism were observed for some time.
authors.affiliation: Institute of Biology for Engineers and Biotechnology of Wastewaters, Am Fasanengarten, University of Karlsruhe, 76131 Karlsruhe, Germany. mini.bajaj@iba.uka.de
authors.forename: Mini
authors.initials: M
authors.lastname: Bajaj
authors.forename: Claudia
authors.initials: C
authors.lastname: Gallert
authors.forename: Josef
authors.initials: J
authors.lastname: Winter
mesh_terms: D000693:Anaerobiosis; D019149:Bioreactors; D005227:Fatty Acids / Q000737:chemistry; D010636:Phenols / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000737:chemistry*
chemical_list: D005227:Fatty Acids; D010636:Phenols; D014874:Water Pollutants, Chemical