Effects of age and jet lag on D-galactose induced aging process.
Chronic administration of D-galactose (D-gal) is a useful method for establishing a model of natural aging. However, whether animal age or jet lag influences the D-gal-induced aging process has not been investigated. To clarify these issues, ICR male mice were given a daily injection of D-gal (500 mg/kg bw) for 7 wks. D-gal-treatment caused a significant decrease only in the transcript level of Cat gene in the 6-wk-old mice, whereas it significantly reduced the expression of Cat and Sod1 genes and of Cat, Gpx1, Sod1 and Sod2 genes in the 12- and 22-wk-old group, respectively, suggesting that D-gal induced aging process was greatly dependent on the age of mice. Mice receiving both D-gal and jet lag exhibited larger decreases in the expression levels of above four genes in liver as compared with that of either group singly, indicating a synergistic effect of jet lag and D-gal on the aging process.
Xu
Yang
Y
College of Biological and Environmental Engineering, Zhejiang University of Technology, No. 6 District, Zhaohui, Hangzhou, Zhejiang, 310032, China.
Wu
Tao
T
Jin
Yuanxiang
Y
Fu
Zhengwei
Z
D000367:Age Factors; D000375:Aging / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D000818:Animals; D002374:Catalase / Q000235:genetics; D005690:Galactose / Q000008:administration & dosage / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005966:Glucuronidase / Q000235:genetics; D005979:Glutathione Peroxidase / Q000235:genetics; D007279:Injections, Subcutaneous; D020179:Jet Lag Syndrome / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology*; D000090265:Klotho Proteins; D008099:Liver / Q000187:drug effects / Q000201:enzymology; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D023421:Models, Animal; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D017440:Photoperiod; D012333:RNA, Messenger / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013482:Superoxide Dismutase / Q000235:genetics; D000072105:Superoxide Dismutase-1; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2
D002374:Catalase; D005690:Galactose; D005966:Glucuronidase; D005979:Glutathione Peroxidase; D000090265:Klotho Proteins; D012333:RNA, Messenger; D013482:Superoxide Dismutase; D000072105:Superoxide Dismutase-1; D000094765:Glutathione Peroxidase GPX1; D000099156:Superoxide Dismutase 2; C000606291:Sod1 protein, mouse; C000722068:Gpx1 protein, mouse