title: An enterovirus strain isolated from diabetic child belongs to a genetic subcluster of echovirus 11, but is also neutralised with monotypic antisera to coxsackievirus A9.
abstract: An enterovirus strain (designated D207) isolated from a Slovakian diabetic child and originally serotyped as coxsackievirus A9 (CAV-9) was found to cause rapid cytolysis coinciding with severe functional damage of the surviving cells in primary cultures of human pancreatic islets. This finding prompted us to clone the isolate for full-length genome sequencing and molecular characterization as the prototype strain of CAV-9 is known to cause only minimal damage to insulin-producing beta-cells. Based on capsid-coding sequence comparisons, the isolate turned out to be echovirus 11 (E-11). Phylogenetic analyses demonstrated that E-11/D207 was closely related to a specific subgroup B of E-11 strains known to cause uveitis. To study further antigenic properties of isolate E-11/D207 and uveitis-causing E-11 strains, neutralization experiments were carried out with CAV-9- and E-11-specific antisera. Unlike the prototype strains, the isolate E-11/D207 and uveitis-causing E-11 strains were well neutralized with both CAV-9- and E-11-specific antisera. Attempts to identify recombination of the capsid coding sequences as a reason for double-reactivity using the Simplot analysis failed to reveal major transferred motifs. However, peptide scanning technique was able to identify antigenic regions of capsid proteins of E-11/D207 as well as regions cross-reacting with an antiserum raised to CAV-9. Thus, double specificity of E-11/D207 seems to be a real characteristic shared by the phylogenetically closely related virus strains in the genetic subgroup B of E-11.
authors.affiliation: Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
authors.forename: Haider
authors.initials: H
authors.lastname: Al-Hello
authors.affiliation: Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
authors.forename: Anja
authors.initials: A
authors.lastname: Paananen
authors.affiliation: Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
authors.forename: Mervi
authors.initials: M
authors.lastname: Eskelinen
authors.affiliation: Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
authors.forename: Petri
authors.initials: P
authors.lastname: Ylipaasto
authors.affiliation: Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
authors.forename: Tapani
authors.initials: T
authors.lastname: Hovi
authors.affiliation: Renal Transplant Unit, Helsinki University Hospital, Helsinki, Finland.
authors.forename: K
authors.initials: K
authors.lastname: Salmela
authors.affiliation: Institute of Poliomyelitis and Viral Encephalitis, Russian Academy of Medical Sciences, Moscow, Russia.
authors.forename: Alexander N
authors.initials: AN
authors.lastname: Lukashev
authors.affiliation: Department of Virology, Slovak Medical University, Bratislava, Slovak Republic.
authors.forename: Shubhada
authors.initials: S
authors.lastname: Bobegamage
authors.affiliation: Enterovirus Laboratory, National Public Health Institute, Helsinki, Finland.
authors.forename: Merja
authors.initials: M
authors.lastname: Roivainen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D000956:Antigens, Viral / Q000737:chemistry / Q000235:genetics / Q000276:immunology; D002478:Cells, Cultured; D002648:Child; D003922:Diabetes Mellitus, Type 1 / Q000821:virology*; D004457:Echovirus Infections / Q000276:immunology / Q000821:virology*; D029822:Enterovirus B, Human / Q000145:classification* / Q000235:genetics / Q000276:immunology* / Q000302:isolation & purification; D004769:Enterovirus Infections / Q000276:immunology / Q000821:virology; D006801:Humans; D007515:Islets of Langerhans / Q000166:cytology / Q000821:virology; D008969:Molecular Sequence Data; D009500:Neutralization Tests; D010449:Peptide Mapping; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D017422:Sequence Analysis, DNA; D012703:Serotyping; D014605:Uveitis / Q000276:immunology / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D000956:Antigens, Viral; D010455:Peptides