Inositol 1,4,5-trisphosphate receptor 1, a widespread Ca2+ channel, is a novel substrate of polo-like kinase 1 in eggs.
To initiate embryo development, the sperm induces in the egg release of intracellular calcium ([Ca2+](i)). During oocyte maturation, the inositol 1,4,5-trisphosphate receptor (IP(3)R1), the channel implicated, undergoes modifications that enhance its function. We found that IP(3)R1 becomes phosphorylated during maturation at an MPM-2 epitope and that this persists until the fertilization-associated [Ca2+](i) responses cease. We also reported that maturation without ERK activity diminishes IP(3)R1 MPM-2 reactivity and [Ca2+](i) responses. Here, we show that IP(3)R1 is a novel target for Polo-like kinase1 (Plk1), a conserved M-phase kinase, which phosphorylates it at an MPM-2 epitope. Plk1 and IP(3)R1 interact in an M-phase preferential manner, and they exhibit close co-localization in the spindle/spindle poles area. This co-localization is reduced in the absence of ERK activity, as the ERK pathway regulates spindle organization and IP(3)R1 cortical re-distribution. We propose that IP(3)R1 phosphorylation by Plk1, and possibly by other M-phase kinases, underlies the delivery of spatially and temporally regulated [Ca2+](i) signals during meiosis/mitosis and cytokinesis.
Ito
Junya
J
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA 01003, USA.
Yoon
Sook-Young
SY
Lee
Bora
B
Vanderheyden
Veerle
V
Vermassen
Elke
E
Wojcikiewicz
Richard
R
Alfandari
Dominique
D
De Smedt
Humbert
H
Parys
Jan B
JB
Fissore
Rafael A
RA
D000818:Animals; D015220:Calcium Channels / Q000378:metabolism*; D020013:Calcium Signaling; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002455:Cell Division; D005260:Female; D053496:Inositol 1,4,5-Trisphosphate Receptors; D051379:Mice; D010063:Ovum / Q000737:chemistry*; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D008941:Spindle Apparatus / Q000737:chemistry; D000097869:Polo-Like Kinase 1
D015220:Calcium Channels; D018797:Cell Cycle Proteins; D053496:Inositol 1,4,5-Trisphosphate Receptors; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D018160:Receptors, Cytoplasmic and Nuclear; D000097869:Polo-Like Kinase 1; C025389:Itpr1 protein, mouse