title: In situ immunocytochemical detection of altered membrane composition induced by cell-cell contact in cultured mammalian cells.
abstract: In cultured mammalian cells, both normal and transformed, cell-cell contact was shown to alter the detergent extractability of nuclear and plasma membranes detected using immunocytochemistry of fixed cells. This alteration occurred in each cell individually, occurred in less than 1 hour after altered cell-cell contact, and did not involve new protein or mRNA synthesis. These results indicate that composition of cellular membranes is highly affected by cell-cell contact and culture density. This suggests a possible role for alterations in membrane composition in the regulation of normal cell behavior, including signaling of contact inhibition of growth and movement, behaviors that are not effectively regulated by cell-cell contact in malignantly transformed cells.
authors.affiliation: Tumor Biology Section, Department of Pathology, Wake Forest University School of Medicine, Winston-Salem, North Carolina 27157, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Du
authors.forename: Zheng
authors.initials: Z
authors.lastname: Cui
authors.forename: Zhao-Chun
authors.initials: ZC
authors.lastname: Tsui
authors.forename: Qirui
authors.initials: Q
authors.lastname: Chen
authors.forename: Mark C
authors.initials: MC
authors.lastname: Willingham
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D002462:Cell Membrane / Q000737:chemistry*; D002463:Cell Membrane Permeability; D004285:Dogs; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D051379:Mice; D018167:Receptors, Calcitriol / Q000032:analysis
chemical_list: D018167:Receptors, Calcitriol