Msx1 and Msx2 are expressed in sub-populations of vascular smooth muscle cells.
Using an nlacZ reporter gene inserted at the Msx1 and Msx2 loci, we could analyze the expression of these homeogenes in the adult mouse. We observed that Msx genes are prominently expressed in a subset of blood vessels. The Msx2nlacZ allele is mainly expressed in a restricted population of mural cells in peripheral arteries and veins. Msx1nlacZ is expressed to a lesser extent by vascular smooth muscle cells of peripheral arteries, but is highly expressed in arterioles and capillaries, making Msx1 a novel marker for a subpopulation of pericytes. Expression is set up early in developing vessels and maintained throughout life. In addition, expression of both genes is observed in a few endothelial cells of the aorta at fetal stages, and only Msx2 continues to be expressed in this layer at the adult stage. These results suggest major functions for Msx genes in vascular mural cell formation and remodeling.
Goupille
Olivier
O
CNRS URA2578, Génétique Moléculaire de la Morphogenèse, Institut Pasteur, Paris, France.
Saint Cloment
Cécile
C
Lopes
Miguel
M
Montarras
Didier
D
Robert
Benoît
B
D000818:Animals; D001011:Aorta / Q000166:cytology / Q000196:embryology; D002196:Capillaries / Q000166:cytology / Q000196:embryology; D004730:Endothelium, Vascular / Q000196:embryology / Q000502:physiology; D005263:Femoral Artery / Q000166:cytology / Q000196:embryology; D018507:Gene Expression Regulation, Developmental; D018398:Homeodomain Proteins / Q000235:genetics* / Q000378:metabolism; D051957:MSX1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008817:Mice, Mutant Strains; D009131:Muscle, Smooth, Vascular / Q000166:cytology / Q000196:embryology* / Q000502:physiology*; D032389:Myocytes, Smooth Muscle / Q000166:cytology / Q000502:physiology; D020286:Pericytes / Q000166:cytology / Q000502:physiology
D018398:Homeodomain Proteins; D051957:MSX1 Transcription Factor; C084119:MSX2 protein; C484084:Msx1 protein, mouse