title: [Induced apoptosis and mechanism of endosulfan in mouse germ cells].
abstract: Endosulfan which is going to be included as POPs in effect of organism have become the focus of international concern. To explore endosulfan on apoptosis of spermatogenic cells and mechanisms, doses of 7.0, 3.5 and 1.75 mg/kg oral administration to mice exposed to 28 consecutive days. Morphology characters of spermatogenic cells observed by laser confocal scanning microscope (LCSM), DNA ladder detected by electricity swims, and increasing of DNA degeneration rate measured through diphenylamine (p < 0.01). Endosulfan might enhance calcium concentration in the spermatogenic cells and restrained Na+ K+ -ATPase and Ca2+ Mg2+ -ATPase activities that showed endosulfan might induce spermatogenic cells in the testicle to occur apoptosis (p < 0.01), to cause the function of transporting Ca2+ to be limited, reduce the ability of excluding calcium and send Ca2+ concentration to hoist sustainingly,i.e. calcium overload in the cells.
authors.affiliation: College of Civil Environmental Engineering, Harbin University of Technology, Harbin 150090, China. rnq@hit.edu.cn
authors.forename: Nan-qi
authors.initials: NQ
authors.lastname: Ren
authors.forename: Xiao-dan
authors.initials: XD
authors.lastname: Zhang
authors.forename: Guang-hong
authors.initials: GH
authors.lastname: Zhou
authors.forename: Shao-jun
authors.initials: SJ
authors.lastname: Chen
mesh_terms: D000284:Administration, Oral; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D017301:Ca(2+) Mg(2+)-ATPase / Q000378:metabolism; D002118:Calcium / Q000378:metabolism; D053938:DNA Fragmentation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004726:Endosulfan / Q000008:administration & dosage / Q000633:toxicity*; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity; D008297:Male; D051379:Mice; D011897:Random Allocation; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013090:Spermatocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism
chemical_list: D007306:Insecticides; D017301:Ca(2+) Mg(2+)-ATPase; D000254:Sodium-Potassium-Exchanging ATPase; D004726:Endosulfan; D002118:Calcium