[Rapid detection of Pseudomonas aernginosa by the fluorescence quantitative TaqMan PCR assay targetting ETA gene].
Pseudomonas aernginosa (PA) is one of the most universal pathogens in clinical diagnosis, and conventional detection assay has many disadvantages. In this research, a pair of specific primers and a TaqMan fluorescent probe were designed in the conservative region of ETA gene by the method of bioinformatics analysis, the detection method for PA was successfully developed. Different gradient concentrations of PA DNA and various pathogen DNA were amplified by fluorescence quantitative PCR (FQ-PCR) to confirm the specificity and sensitivity of the developed method. Results showed that the developed detection assay is more sensible and specific by comparison to the conventional FQ-PCR method, and it is valuable for research and application prospects.
Xiao
Xinglong
X
College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China.
Zhang
Jingwei
J
Gong
Jun
J
Pan
Yanping
Y
Yu
Yigang
Y
Yang
Xiaoquan
X
Wu
Hui
H
D036002:ADP Ribose Transferases / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D004269:DNA, Bacterial / Q000032:analysis*; D005098:Exotoxins / Q000235:genetics*; D005456:Fluorescent Dyes; D005470:Fluorometry / Q000379:methods; D016133:Polymerase Chain Reaction / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000235:genetics / Q000302:isolation & purification*; D012680:Sensitivity and Specificity; D019914:Taq Polymerase; D037521:Virulence Factors / Q000235:genetics*; D000097668:Pseudomonas aeruginosa Exotoxin A
D036002:ADP Ribose Transferases; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D005098:Exotoxins; D005456:Fluorescent Dyes; D019914:Taq Polymerase; D037521:Virulence Factors; D000097668:Pseudomonas aeruginosa Exotoxin A