title: Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium.
abstract: Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE.
authors.affiliation: College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, South Korea.
authors.forename: Yong Kwon
authors.initials: YK
authors.lastname: Lee
authors.forename: Seung Yeul
authors.initials: SY
authors.lastname: Kim
authors.forename: Ki Heon
authors.initials: KH
authors.lastname: Kim
authors.forename: Bok-Hwan
authors.initials: BH
authors.lastname: Chun
authors.forename: Kweon-Haeng
authors.initials: KH
authors.lastname: Lee
authors.forename: Duk Jae
authors.initials: DJ
authors.lastname: Oh
authors.forename: Namhyun
authors.initials: N
authors.lastname: Chung
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D030262:Soybean Proteins / Q000378:metabolism / Q000494:pharmacology*
chemical_list: D016895:Culture Media, Serum-Free; D030262:Soybean Proteins; D019344:Lactic Acid; D004247:DNA; D005947:Glucose