alpha-Glucosidase inhibitory activity of cyanidin-3-galactoside and synergistic effect with acarbose.
Cyanidin-3-galactoside, a natural anthocyanin, was investigated for its alpha-glucosidase inhibitory activity. The IC(50) value of cyanidin-3-galactoside was 0.50 +/- 0.05 mM against intestinal sucrase. A low dose of cyanidin-3-galactoside showed a synergistic inhibition on intestinal alpha-glucosidase (maltase and sucrase) when combined with acarbose. A kinetic analysis showed that cyanidin-3-galactoside gave a mixed type inhibition against intestinal sucrase. The results indicated that cyanidin-3-galactoside was an alpha-glucosidase inhibitor and could be used in combination with acarbose for treatment of diabetes.
Adisakwattana
Sirichai
S
Department of Transfusion Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand. adisakwattana_siri@yahoo.com
Charoenlertkul
Piyawan
P
Yibchok-Anun
Sirintorn
S
D020909:Acarbose / Q000494:pharmacology*; D000818:Animals; D000872:Anthocyanins / Q000494:pharmacology*; D004357:Drug Synergism; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; D020128:Inhibitory Concentration 50; D007422:Intestines / Q000201:enzymology; D007700:Kinetics; D051381:Rats; D013393:Sucrase / Q000037:antagonists & inhibitors
D000872:Anthocyanins; D004791:Enzyme Inhibitors; D065089:Glycoside Hydrolase Inhibitors; D007004:Hypoglycemic Agents; C017154:cyanidin; D013393:Sucrase; D020909:Acarbose