Red light-activated phosphorothioate oligodeoxyribonucleotides.
Hairpin-structured phosphorothioate oligodeoxyribonucleotides containing a singlet oxygen-sensitive linker in the loop were prepared. These compounds do not bind complementary nucleic acids in the dark. Upon irradiation with red light in the presence of chlorine e6 the linker within these compounds is cleaved and a single-stranded oligodeoxyribonucleotide is produced. The latter compound is an efficient binder of complementary nucleic acids. This is the first example of 'caged' phosphorothioate oligodeoxyribonucleotides, whose nucleic acid binding ability is triggered by red light.
Rotaru
Alexandru
A
Inorganic Chemistry Institute, University of Heidelberg, In Neuenheimer Feld 270, 69120 Heidelberg, Germany.
Kovács
János
J
Mokhir
Andriy
A
D008027:Light; D015394:Molecular Structure; D009838:Oligodeoxyribonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D054735:Phosphorothioate Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D026082:Singlet Oxygen / Q000737:chemistry
D009838:Oligodeoxyribonucleotides; D054735:Phosphorothioate Oligonucleotides; D026082:Singlet Oxygen