[Expression of odontogenic and osteogenic genes in bone marrow mesenchymal stem cells with overexpression of mouse dentin sialophosphoprotein].
OBJECTIVE
To observe the overexpression of dentin sialophosphoprotein (DSPP) in mouse bone marrow mesenchymal stem cells (BM-MSC) and the expression of specific gene involved in odontogenic and osteogenic differentiation in transfected cells.


METHODS
Mouse bone marrow mesenchymal stem cells (BM-MSC) were cultured and then transfected with DSPP gene by adenovirus-mediated way in vitro. The transfection efficiency of DSPP gene was assessed by the marker gene green fluorescent protein (GFP). With having observed the morphological transformation of transfected BM-MSC, we studied whether the transfected BM-MSC would express odontogenic and osteogenic genes by RT-PCR.


RESULTS
The mouse BM-MSC were obtained and the adenovirus mediated DSPP gene used to transfect BM-MSC successfully. The transfected efficiency was 42.7%. The transfected BM-MSC were induced to differentiate into odontoblast-like cells and meanwhile expressed specific odontogenic and osteogenic differentiation genes such as DSPP, DMP1, Msx1/2, Pax9, Lhx6/7, Sox9, Cbfa1, Osx, Col I.


CONCLUSIONS
The bone marrow mesenchymal stem cells can make differentiation with the overexpression of dentin sialophosphoprotein and express specific odontogenic and osteogenic differentiation genes.
Zhu
Feng
F
State Key Laboratory of Oral Diseases, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Nie
Rong-Rong
RR
Wu
Ling
L
Liu
Lei
L
Tang
Wei
W
Tian
Wei-Dong
WD
D000256:Adenoviridae / Q000235:genetics; D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology* / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D003804:Dentin / Q000166:cytology; D015870:Gene Expression; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008856:Microscopy, Fluorescence; D009804:Odontoblasts / Q000166:cytology / Q000378:metabolism; D009805:Odontogenesis / Q000235:genetics*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D010012:Osteogenesis / Q000235:genetics*; D010750:Phosphoproteins / Q000096:biosynthesis / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins / Q000235:genetics* / Q000378:metabolism; D014162:Transfection
D010750:Phosphoproteins; D012333:RNA, Messenger; D011993:Recombinant Fusion Proteins; D012795:Sialoglycoproteins; D049452:Green Fluorescent Proteins