GSTM, GSTT and p53 polymorphisms as modifiers of clinical outcome in colorectal cancer.
BACKGROUND
Cancer of the colorectal region is the second most frequent cause of death among malignant diseases. The influence of two allelic polymorphisms of GSTM1 and GSTT1, and that of p53 gene codon 72 on colon cancer was investigated.


METHODS
Intraoperatively removed tissue samples were processed from colorectal cancer patients. Cancer-free human samples were used as matched controls. Samples were digested with proteinase-K. DNA solution was used for PCR amplification.


RESULTS
No significant difference was found between tumor patients and controls in the investigated polymorphisms. A significant association was found in Dukes' B stage patients between the GSTM1 and p53 gene variants and survival. In patients with GSTM1 null genotype and p53 Arg/Pro heterozygotes or Pro/Pro homozygotes the chance of survival is significantly lower than in the case of GSTM1+ and p53 Arg/Arg variants (p=0.009 and p=0.008, respectively).


CONCLUSIONS
The significance of the investigated polymorphisms in prognosis is dependent on the tumor stage. These parameters might be used in certain cases as prognostic biomarkers in clinical diagnostics and in the planning of individual therapy.
Csejtei
Andras
A
Department of Oncoradiology, Markusovszky Hospital, Markusovszky, Hungary.
Tibold
Antal
A
Varga
Zsuzsa
Z
Koltai
Katalin
K
Ember
Agoston
A
Orsos
Zsuzsa
Z
Feher
Gergely
G
Horvath
Ors Peter
OP
Ember
Istvan
I
Kiss
Istvan
I
D000230:Adenocarcinoma / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D000483:Alleles; D015179:Colorectal Neoplasms / Q000201:enzymology / Q000235:genetics* / Q000473:pathology; D005260:Female; D016158:Genes, p53; D005982:Glutathione Transferase / Q000235:genetics*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011110:Polymorphism, Genetic; D015996:Survival Rate; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
D005982:Glutathione Transferase; D016159:Tumor Suppressor Protein p53; C495901:TP53 protein, human; C413545:glutathione S-transferase T1