[Determination of the total content of astragaloside IV in Radix Astragali].
OBJECTIVE
To determine the total content of astragaloside IV in Radix Astragali.


METHODS
The measurement conditions were used as follows: Irregular C18 (4.6 mm x 250 mm, 5 microm) column; mobile phase: acetonitrile-water (32:68); flow rate: 1.0 ml/min; detector: ELSD 2000; the temperature of drift tube: 100 degrees C; gas flow: 2.7 L/min.


RESULTS
Eight batches of Radix Astragali from different sources were determined. The stability, precision and reproducibility of the method were studied, RSD <3%.


CONCLUSIONS
There is great difference between the content of astragaloside IV in Radix Astragali by different processing methods.
Xia
Guang-Ping
GP
Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China. guangpingx@163.com
Liu
Peng
P
Han
Ying-Mei
YM
D027885:Astragalus propinquus / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D010598:Pharmacognosy / Q000379:methods / Q000592:standards; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000302:isolation & purification; D013696:Temperature; D014315:Triterpenes / Q000032:analysis* / Q000302:isolation & purification
D012503:Saponins; D014315:Triterpenes; C052064:astragaloside A