Biochemical properties of a persistent nonoccluded baculovirus isolated from Heliothis zea cells.
A persistent nonoccluded rod-shaped nuclear virus of Heliothis zea (IMC-Hz-1-NOV) was isolated from Trichoplusia ni cells (Tn-368) by sucrose density gradient centrifugation. The molecular weights of IMC-Hz-1-NOV structural proteins were determined by SDS-polyacrylamide gel electrophoresis. Separation of IMC-Hz-1-NOV DNA restriction fragments by agarose gel electrophoresis gave an approximate DNA molecular weight of 132 x 10(6). Digestion of double-stranded IMC-Hz-1-NOV DNA with HindIII and BamHI revealed a cleavage pattern different from the patterns of H. zea NPV and H. armigera NPV.
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Boyce Thompson Institute, Cornell University, Tower Road, Ithaca, New York 14853, USA.