title: Detection of ciguatoxin in fish tissue using sandwich ELISA and neuroblastoma cell bioassay.
abstract: The applicability of a new enzyme-linked immunoassay (ELISA) for detecting ciguatoxin (CTX) in fish tissue was evaluated by testing three fish species commonly implicated in ciguatera fish poisoning in Hawaii. A total of 164 individual almaco jack (Seriola rivoliana) and greater amberjack (S. dumerili) and a total of 175 individuals of the blue-spotted grouper (Cephalopholis argus) were caught at various locations in the Hawaiian Islands. Muscle tissue from each individual was assessed for the presence of CTX using two methods: a semi-quantitative ELISA that was recently developed for detecting picogram levels of CTX in fish extract and a neuroblastoma (NB) cell assay commonly used to screen for marine toxins in fish. Results of the tests were highly correlated, with the ELISA indicating the presence of CTX in 9.4% of all fish samples, and the NB assay indicating toxicity in 6.8% of the fish samples. We conclude that the ELISA produces reliable and accurate results that are consistent with those provided by the accepted NB assay and that the ELISA has potential for future applications in screening fish populations for CTX.
authors.affiliation: Department of Pathology, John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822, USA. empey@hawaii.edu
authors.forename: Cara
authors.initials: C
authors.lastname: Empey Campora
authors.forename: Jan
authors.initials: J
authors.lastname: Dierking
authors.forename: Clyde S
authors.initials: CS
authors.lastname: Tamaru
authors.forename: Yoshitsugi
authors.initials: Y
authors.lastname: Hokama
authors.forename: Douglas
authors.initials: D
authors.lastname: Vincent
mesh_terms: D000818:Animals; D001681:Biological Assay; D002470:Cell Survival / Q000187:drug effects; D036841:Ciguatera Poisoning / Q000175:diagnosis*; D002922:Ciguatoxins / Q000032:analysis / Q000494:pharmacology*; D004784:Environmental Monitoring / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D051379:Mice; D018482:Muscle, Skeletal / Q000737:chemistry; D009447:Neuroblastoma / Q000188:drug therapy / Q000473:pathology; D010473:Perciformes; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D014407:Tumor Cells, Cultured
chemical_list: D002922:Ciguatoxins