HPLC-electrospray mass spectrometric assay for the determination of (R,R)-fenoterol in rat plasma.
A fast and specific liquid chromatography-mass spectrometry method for the determination of (R,R)-fenoterol ((R,R)-Fen) in rat plasma has been developed and validated. (R,R)-Fen was extracted from 125 microl of plasma using solid phase extraction and analyzed on Atlantis HILIC Silica 3 microm column. The mobile phase was composed of acetonitrile:ammonium acetate (pH 4.1; 20mM) (85:15, v/v), at a flow rate of 0.2 ml/min. The lower limit of detection (LLOD) was 2 ng/ml . The procedure was validated and applied to the analysis of plasma samples from rats previously administered (R,R)-Fen in an intravenous bolus.
Siluk
Danuta
D
Laboratory of Clinical Investigation, Gerontology Research Center, National Institute on Aging, NIH, Baltimore, MD 21224, USA. silukd@mail.nih.gov
Kim
Hee Seung
HS
Cole
Tyler
T
Wainer
Irving W
IW
D000318:Adrenergic beta-Agonists / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D029721:Drosophila Proteins; D004355:Drug Stability; D005280:Fenoterol / Q000097:blood* / Q000737:chemistry / Q000493:pharmacokinetics; D005615:Freezing; D008297:Male; D015394:Molecular Structure; D011786:Quality Control; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D017413:Ribonucleoprotein, U2 Small Nuclear; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013997:Time Factors
D000318:Adrenergic beta-Agonists; D029721:Drosophila Proteins; C523272:RAD201 protein, Drosophila; D017413:Ribonucleoprotein, U2 Small Nuclear; D005280:Fenoterol