Detection of Corynebacterium kutscheri in the faeces of subclinically infected mice.
Mice were infected experimentally and subclinically with Corynebacterium kutscheri to recover the organism from mice faeces. The faeces were then cultured using selective furazolidone-nalidixic acid-colimycin agar. The number of C. kutscheri per gram of fresh faeces varied from mouse to mouse, but once established in the intestine, the organism was excreted in the faeces for at least five months. Viable bacteria were detected in most of the faecal samples, including those stored in the animal room for five days. The number of organisms in the stored faeces decreased gradually but did not differ significantly from those in the fresh faeces until they had been stored for more than three days. Many infected mice excreted between 10(4.77) and 10(5.37) colony forming units (CFU) of C. kutscheri per day in their faeces, and one mouse even excreted 10(3.74) CFU at eight weeks postinfection. These values showed little daily variation. Our present study showed that subclinically infected mice discharged the organism continuously and persistently in their faeces. Therefore, faecal samples would be useful for monitoring infection with C. kutscheri in living mice in a manner that is not stressful for the animals.
Amao
H
H
Laboratory of Experimental Animal Science, Nippon Veterinary and Life Science University, 1-7-1 Kyonan-cho, Musashino-shi, Tokyo, Japan. amao@nvlu.av.jp
Moriguchi
N
N
Komukai
Y
Y
Kawasumi
H
H
Takahashi
K
K
Sawada
T
T
D000818:Animals; D000830:Animals, Laboratory; D015169:Colony Count, Microbial / Q000662:veterinary; D003352:Corynebacterium / Q000254:growth & development / Q000302:isolation & purification*; D003354:Corynebacterium Infections / Q000382:microbiology / Q000662:veterinary*; D005243:Feces / Q000382:microbiology*; D007410:Intestinal Diseases / Q000382:microbiology / Q000662:veterinary*; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D012376:Rodent Diseases / Q000382:microbiology*; D013047:Specific Pathogen-Free Organisms