Virus multiplication in tobacco protoplasts inoculated with tobacco mosaic virus RNA encapsulated in large unilamellar vesicle liposomes.
Tobacco mosaic virus RNA encapsulated in large unilamellar vesicles of phosphatidylserine was found to infect efficiently tobacco protoplasts prepared from suspension culture cells. Multiplication of tobacco mosaic virus in infected cells was confirmed by fluorescent antibody staining, electron microscopic observation, and infectivity assay. Virus infectivity was first detected at 6 hr after infection, it then increased rapidly and reached a plateau at 20 to 24 hr.
Watanabe
Y
Y
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Tokyo 118, Japan.
Ohno
T
T
Okada
Y
Y