The distribution of virus-specific RNA in Rous sarcoma virus-induced hamster tumor cells.
Poly(A)-containing cytoplasmic RNA and nuclear RNA from Rous sarcoma virus (RSV)-induced hamster tumor cells was fractionated on sucrose density gradients and hybridized with a [3H]cDNA probe to determine the size distribution of virus-specific RNA. One size class of viral RNA sedimenting at 21 S was detected in cytoplasmic poly(A)-containing RNA. Nuclear RNA contained three species of virus-specific RNA sedimenting at 47, 35, and 21 S. Sedimentation of nuclear RNA under denaturing conditions did not change this size distribution.
Brugge
J S
JS
Department of Pathology, University of Colorado Medical Center, Denver, Colorado 80262, USA.
Purchio
A F
AF
Erikson
R L
RL