title: Nitric oxide administration restores the hepatic artery buffer response during porcine endotoxemia.
abstract: The hepatic artery buffer response, which is lost during endotoxemia, plays a central role in the autoregulation of liver perfusion. A temporarily decreased synthesis of nitric oxide during early endotoxemia might be responsible for this dysfunction; hence exogenous administration of nitric oxide could reestablish the autoregulation of hepatic blood flow and help prevent hepatic damage later in septic shock. Fifteen pigs were treated with lipopolysaccharide +/- the nitric oxide donor nitroprusside-sodium via the portal vein. Hemodynamics were measured, and serum chemistry and liver biopsies for nitric oxide synthase expression were obtained. Lipopolysaccharide decreased arterial liver perfusion after 5 hours by 38% (p = .012), which was reversed by addition of nitroprusside (8%). Administration of nitroprusside preserved an increase of 28% in hepatic arterial upon portal vein flow reduction (p < .001). Nitroprusside maintained mRNA levels of constitutive nitric oxide synthase in liver tissue which were decreased by lipopolysaccharide (p = .026 vs. p = .114) and tempered the burst in inducible nitric oxide synthase expression at t = 3 hours. The early administration of the nitric oxide donor sodium nitroprusside during endotoxemia is able to reestablish the autoregulatory response of the hepatic artery following reduction of hepatic blood flow. This beneficial effect might help to prevent subsequent hepatic damage in the course of abdominal sepsis.
authors.affiliation: Department of Surgery, Vienna General Hospital, Medical University of Vienna, Vienna, Austria.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Tamandl
authors.forename: Pål
authors.initials: P
authors.lastname: Jørgensen
authors.forename: Yngvar
authors.initials: Y
authors.lastname: Gundersen
authors.forename: Reinhold
authors.initials: R
authors.lastname: Fuegger
authors.forename: Thomas
authors.initials: T
authors.lastname: Sautner
authors.forename: Ansgar O
authors.initials: AO
authors.lastname: Aasen
authors.forename: Peter
authors.initials: P
authors.lastname: Goetzinger
mesh_terms: D000136:Acid-Base Equilibrium / Q000187:drug effects; D000818:Animals; D019446:Endotoxemia / Q000503:physiopathology*; D005260:Female; D006439:Hemodynamics / Q000187:drug effects; D006499:Hepatic Artery / Q000502:physiology*; D008026:Ligation; D008102:Liver Circulation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D009569:Nitric Oxide / Q000627:therapeutic use*; D052248:Nitric Oxide Synthase Type I / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D009599:Nitroprusside; D011169:Portal Vein / Q000502:physiology; D012333:RNA, Messenger / Q000378:metabolism; D034421:Sus scrofa; D014655:Vascular Resistance / Q000187:drug effects
chemical_list: D012333:RNA, Messenger; D009599:Nitroprusside; D009569:Nitric Oxide; D052248:Nitric Oxide Synthase Type I; D052247:Nitric Oxide Synthase Type II