title: Leucine-rich glioma inactivated 3 is involved in amyloid beta peptide uptake by astrocytes and endocytosis itself.
abstract: We earlier showed that leucine-rich glioma inactivated 3 (LGI3) colocalizes with amyloid beta peptide (Abeta) taken up by astrocytes both in vitro and in vivo, and that LGI3 accumulated with endocytosis-associated proteins in aged monkey brains. In this study, we confirmed that LGI3 localizes to the endocytic pathway and found that its accumulation is caused by endocytic perturbation. Most notably, RNA interference experiments demonstrated that the downregulation of LGI3 clearly inhibited Abeta uptake by cultured rat astrocytes, moreover, transferrin uptake by both astrocytes and neuronal cells. Together with our earlier findings, our results suggest that LGI3 is involved in Abeta uptake by astrocytes and even endocytosis itself.
authors.affiliation: aLaboratory of Disease Control, Tsukuba Primate Research Center, National Institute of Biomedical Innovation, Tsukuba-shi, Ibaraki 305-0843, Japan.
authors.forename: Sachi
authors.initials: S
authors.lastname: Okabayashi
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kimura
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D004705:Endocytosis; D005260:Female; D007150:Immunohistochemistry; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism; D011247:Pregnancy; D034622:RNA Interference; D051381:Rats; D017207:Rats, Sprague-Dawley; D014168:Transferrin / Q000378:metabolism
chemical_list: D016229:Amyloid beta-Peptides; C511168:LGI3 protein, mouse; D009419:Nerve Tissue Proteins; D014168:Transferrin