title: Antibacterial coatings for medical devices based on glass polyalkenoate cement chemistry.
abstract: A biofilm is an accumulation of micro-organisms and their extracellular products forming a structured community on a surface. Biofilm formation on medical devices has severe health consequences as bacteria growing in this lifestyle are tolerant to both host defense mechanisms and antibiotic therapies. However, silver and zinc ions inhibit the attachment and proliferation of immature biofilms. The objective of this study is to evaluate whether it is possible to produce silver and zinc-containing glass polyalkenoate cement (GPC) coatings for medical devices that have antibacterial activity and which may therefore inhibit biofilm formation on a material surface. Two silver and zinc-containing GPC coatings (A and B) were synthesised and coated onto Ti6Al4V discs. Their handling properties were characterised and atomic absorption spectrometery was employed to determine zinc and silver ion release with coating maturation up to 30 days. The antibacterial properties of the coatings were also evaluated against Staphylococcus aureus and a clinical isolate of Pseudomonas aeruginosa using an agar diffusion assay method. The majority of the zinc and silver ions were released within the first 24 h; both coatings exhibited antibacterial effect against the two bacterial strains, but the effect was more intense for B which contained more silver and less zinc than A. Both coatings produced clear zones of inhibition with each of the two organisms tested. In this assay, Ps. aeruginosa was more sensitive than S. aureus. The diameters of these zones were reduced after the coating had been immersed in water for varying periods due to the resultant effect on ion release.
authors.affiliation: Clinical Materials Unit & Materials and Surface Science Institute, University of Limerick, National Technological Park, Limerick, Ireland.
authors.forename: A
authors.initials: A
authors.lastname: Coughlan
authors.forename: D
authors.initials: D
authors.lastname: Boyd
authors.forename: C W I
authors.initials: CW
authors.lastname: Douglas
authors.forename: M R
authors.initials: MR
authors.lastname: Towler
mesh_terms: D000497:Alloys / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology; D018441:Biofilms / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D004864:Equipment and Supplies / Q000382:microbiology*; D005899:Glass Ionomer Cements / Q000737:chemistry* / Q000494:pharmacology; D008826:Microbial Sensitivity Tests; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000737:chemistry; D013211:Staphylococcus aureus / Q000187:drug effects; D013499:Surface Properties; D014961:X-Ray Diffraction; D015032:Zinc / Q000737:chemistry
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D005899:Glass Ionomer Cements; D012834:Silver; D015032:Zinc