title: Improved assay for determining the total radical-scavenging capacity of antioxidants and foods.
abstract: Free radicals play a crucial role in the pathophysiology of human diseases such as cancer, atherosclerosis, and neurodegenerative diseases, and considerable attention has been focused on functional foods (or nutraceuticals) that are able to decrease the concentrations of free radicals and consequently protect against these diseases. The present study investigated an improved quantitative assay to measure antioxidant activity using the stable and fast-reacting chromogenic indicator [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS). The ABTS-radical-scavenging activities of various antioxidants and apple extracts were measured in 96-well plates, and plots thereof were linearly interpolated, with the total radical-scavenging capacity quantified as the area under the curve. The first order of linear regression was obtained in a relationship between the absorbance reduction and various concentrations of the tested sample, and the total radical-scavenging capacity was expressed as the vitamin-C-equivalent antioxidant capacity. The advantages of this quantitative assay are that, first, it is fast, sensitive and confers little variation from experimental errors for single or mixed antioxidants; second, a large number of samples in a low quantity at a time can be run using 96-well plates.
authors.affiliation: Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
authors.forename: Ki Won
authors.initials: KW
authors.lastname: Lee
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Kim
authors.forename: Nam Joo
authors.initials: NJ
authors.lastname: Kang
authors.forename: Jong Hun
authors.initials: JH
authors.lastname: Kim
authors.forename: Sang Jun
authors.initials: SJ
authors.lastname: Lee
authors.forename: Dae-Ok
authors.initials: DO
authors.lastname: Kim
authors.forename: Chang Yong
authors.initials: CY
authors.lastname: Lee
authors.forename: Hyong Joo
authors.initials: HJ
authors.lastname: Lee
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000032:analysis / Q000494:pharmacology; D052160:Benzothiazoles; D002083:Butylated Hydroxyanisole / Q000032:analysis / Q000494:pharmacology; D002863:Chromogenic Compounds; D003124:Colorimetry / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D016166:Free Radical Scavengers / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D044949:Kaempferols / Q000032:analysis / Q000494:pharmacology; D027845:Malus / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D013451:Sulfonic Acids
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D002863:Chromogenic Compounds; D016166:Free Radical Scavengers; D044949:Kaempferols; D010936:Plant Extracts; D013451:Sulfonic Acids; D002083:Butylated Hydroxyanisole; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C012526:quercitrin; C006552:kaempferol; D011794:Quercetin; D001205:Ascorbic Acid