title: Transplantation of meniscus regenerated by tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells derived from rat bone marrow.
abstract: The purpose of this study was to assess transplantation of regenerated menisci using scaffolds from normal allogeneic menisci and bone-marrow-derived mesenchymal stromal cells (BM-MSCs) of rats. We reported that scaffolds derived from normal menisci seeded with BM-MSCs in vitro could form meniscal tissues within 4 weeks. Then, we hypothesized that our tissues could be more beneficial than allogeneic menisci regarding early maturation and chondroprotective effect. Bone marrow was aspirated from enhanced green fluorescent protein transgenic rats. BM-MSCs were isolated and seeded onto scaffolds which were prepared from Sprague-Dawley rat menisci. After 4 weeks in coculture, the tissues were transplanted to the defect of menisci. Repopulation of BM-MSCs and expression of extracellular matrices were observed in the transplanted tissues at 4 weeks after surgery. At 8 weeks, articular cartilage in the cell-free group was more damaged compared to that in the cell-seeded group or the meniscectomy group.
authors.affiliation: Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. tacyama@hiroshima-u.ac.jp
authors.forename: Takuma
authors.initials: T
authors.lastname: Yamasaki
authors.forename: Masataka
authors.initials: M
authors.lastname: Deie
authors.forename: Rikuo
authors.initials: R
authors.lastname: Shinomiya
authors.forename: Yuji
authors.initials: Y
authors.lastname: Yasunaga
authors.forename: Shinobu
authors.initials: S
authors.lastname: Yanada
authors.forename: Mitsuo
authors.initials: M
authors.lastname: Ochi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D048091:Guided Tissue Regeneration; D008297:Male; D008592:Menisci, Tibial / Q000502:physiology / Q000637:transplantation*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D017154:Stromal Cells; D046509:Tissue Culture Techniques; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D049452:Green Fluorescent Proteins