Comparative efficacy of standard AGID and precipitinogen inhibition test with monoclonal antibodies based competitive ELISA for the serology of Peste des Petits Ruminants in sheep and goats.
This project was conducted to investigate the comparative efficiency of competitive ELISA (cELISA), standard Agar Gel Immunodiffusion Test (AGID) and Precipitinogen Inhibition Test (PIT) for the diagnosis of Peste des Petits Ruminants (PPR) in Pakistan. To deal with this, serum samples from 198 sheep and 82 goats were collected from three different government livestock farms and all the samples were run simultaneously with the three serological tests. The samples found positive for PPR antibodies through cELISA, AGID and PIT were 96 (34.2%), 60 (21.4%) and 72 (25.7%), respectively. Kappa statistics were applied to evaluate the concordance between the laboratory-based test (cELISA) and field-based tests (AGID and PIT). Kappa statistics scores for cELISA versus AGID and PIT were 0.6343 (95% Confidence Interval CI 0.5231-0.7456) and 0.7134 (95% Confidence Interval CI 0.5987-0.8281), respectively, which indicate a "substantial" agreement between cELISA and AGID and "significant" agreement between cELISA and PIT. AGID and PIT revealed relative diagnostic sensitivities with cELISA of 59.3% and 69.7% and relative diagnostic specificities of 98.3% and 97.2%, respectively. The data suggested that for mass screening and control of PPR, these serological tests proved practical in the absence of cELISA since they have high relative diagnostic specificities and a satisfactory relative diagnostic sensitivities.
Munir
Muhammad
M
Department of Veterinary Microbiology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, 38040, Pakistan. muhammad.munir@bvf.slu.se
Siddique
Muhammad
M
Ali
Qurban
Q
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D000914:Antibodies, Viral / Q000032:analysis / Q000097:blood; D001667:Binding, Competitive / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000592:standards / Q000662:veterinary; D005260:Female; D015511:Goat Diseases / Q000175:diagnosis* / Q000276:immunology; D006041:Goats; D005779:Immunodiffusion / Q000379:methods / Q000592:standards / Q000662:veterinary; D008297:Male; D029021:Peste-des-Petits-Ruminants / Q000175:diagnosis / Q000276:immunology / Q000662:veterinary*; D018107:Peste-des-petits-ruminants virus / Q000276:immunology*; D011233:Precipitin Tests / Q000379:methods / Q000662:veterinary; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods / Q000592:standards / Q000662:veterinary*; D012756:Sheep; D012757:Sheep Diseases / Q000175:diagnosis* / Q000276:immunology
D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral