The first step into the brain: uptake of NIO-PBCA nanoparticles by endothelial cells in vitro and in vivo, and direct evidence for their blood-brain barrier permeation.
By using fluorescent polysorbate 80 coated poly(n-butylcyanoacrylate) (PBCA) nanoparticles in an in vivo study, direct evidence was found for the presence of nanoparticles entering the brain and retina of rats. The nanoparticles, prepared with a miniemulsion process, were labeled in situ with a fluorescent dye and coated with polysorbate 80. After preparation the particle size, zeta potential, and the molecular weight distribution were determined. BMEC cells were used as an in vitro model for the BBB. The cells showed significant uptake of the particles, but no transcytosis could be observed in vitro. After applying the particles to the animals at two concentrations, cryosections of the brains and retinas were prepared. Regarding the sections of the rats that received the lower dose, co-localization of the applied fluorescent particles and the stained endothelial cells could be detected in the brain and retina, indicating particle internalization in the endothelial cells. Applied at higher doses, the particles could be detected within the brain and retina with few co-localized signals, suggesting passage through the blood-brain and blood-retina barriers.
Weiss
Clemens K
CK
Institute of Organic Chemistry III, Macromolecular Chemistry and Organic Materials, University of Ulm, Albert-Einstein-Allee 11, 89081 Ulm, Germany. clemens.weiss@uni-ulm.de
Kohnle
Maria-Verena
MV
Landfester
Katharina
K
Hauk
Thomas
T
Fischer
Dietmar
D
Schmitz-Wienke
Julia
J
Mailänder
Volker
V
D000818:Animals; D001812:Blood-Brain Barrier / Q000187:drug effects* / Q000378:metabolism; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D016503:Drug Delivery Systems; D004659:Enbucrilate / Q000737:chemistry / Q000493:pharmacokinetics*; D042783:Endothelial Cells / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry; D007150:Immunohistochemistry; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011136:Polysorbates / Q000737:chemistry / Q000493:pharmacokinetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012160:Retina / Q000166:cytology / Q000378:metabolism; D013499:Surface Properties; D013501:Surface-Active Agents / Q000737:chemistry / Q000493:pharmacokinetics; D014018:Tissue Distribution
D005456:Fluorescent Dyes; D011136:Polysorbates; D013501:Surface-Active Agents; D004659:Enbucrilate