title: Restriction fragment length polymorphism analysis and direct sequencing for determination of HBV genotypes in a Turkish population.
abstract: The aim of this study was to analyze the restriction fragment length polymorphism and direct sequencing results in genotyping of hepatitis B virus from a Turkish population in a clinical virology laboratory. Serum samples of 54 chronic hepatitis B patients attending the Ege University Hospital were studied. Sequences of partial S gene PCR products were analysed and RFLP was performed. Fifty-three isolates could be identified by direct sequencing as genotype D. One sample needed to be cloned and determined as genotype D. Forty-two isolates were genotyped as D with RFLP according to published determinative patterns. Twelve isolates had undefined patterns. Eight of them suggested a mixture of isolates with different patterns and cloning of these samples confirmed the presence of heterogeneous isolates. Four isolates with undefined pattern were determined as genotype D by direct sequencing. All the studied isolates were genotype D. The results of this studied population suggest that RFLP is suitable for HBV genotyping in a routine clinical virology laboratory setting. However sequence analysis and even cloning may be needed to clarify indeterminate results.
authors.affiliation: Ege University Medical Faculty, Department of Microbiology and Clinical Microbiology, Izmir, Turkey. ruchan.sertoz@ege.edu.tr
authors.forename: Ruchan Yazan
authors.initials: RY
authors.lastname: Sertoz
authors.forename: Selda
authors.initials: S
authors.lastname: Erensoy
authors.forename: Suzan
authors.initials: S
authors.lastname: Pas
authors.forename: Tijen
authors.initials: T
authors.lastname: Ozacar
authors.forename: Hubert
authors.initials: H
authors.lastname: Niesters
mesh_terms: D016000:Cluster Analysis; D016172:DNA Fingerprinting; D004279:DNA, Viral / Q000235:genetics; D005838:Genotype; D006514:Hepatitis B Surface Antigens / Q000235:genetics; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D019694:Hepatitis B, Chronic / Q000821:virology*; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA / Q000379:methods*; D014421:Turkey
chemical_list: D004279:DNA, Viral; D006514:Hepatitis B Surface Antigens