Influence of high solid concentration on enzymatic hydrolysis and fermentation of steam-exploded corn stover biomass.
Steam-exploded corn stover biomass was used as the substrate for fed-batch separate enzymatic hydrolysis and fermentation (SHF) to investigate the solid concentration ranging from 10% to 30% (w/w) on the lignocellulose enzymatic hydrolysis and fermentation. The treatment of washing the steam-exploded material was also evaluated by experiments. The results showed that cellulose conversion changed little with increasing solid concentration, and fermentation by Saccharomyces cerevisiae revealed a nearly same ethanol yield with the water-washed steam-exploded corn stover. For the washed material at 30% substrate concentration, i.e., 30% water insoluble solids (WIS), enzymatic hydrolysis yielded 103.3 g/l glucose solution and a cellulose conversion of 72.5%, thus a high ethanol level up to 49.5 g/l. With the unwashed steam-exploded corn stover, though a cellulose conversion of 70.9% was obtained in hydrolysis at 30% solid concentration (27.9% WIS), its hydrolysate did not ferment at all, and the hydrolysate of 20% solid loading containing 3.3 g/l acetic acid and 145 mg/l furfural already exerted a strong inhibition on the fermentation and ethanol production.
Lu
Yifeng
Y
East China University of Science and Technology, Shanghai, China.
Wang
Yonghong
Y
Xu
Guoqian
G
Chu
Ju
J
Zhuang
Yingping
Y
Zhang
Siliang
S
D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D002480:Cellulase / Q000737:chemistry*; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D008031:Lignin / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000378:metabolism*; D003313:Zea mays / Q000737:chemistry*
C036909:lignocellulose; D000431:Ethanol; D008031:Lignin; D002480:Cellulase