title: "Molded" rods of macroporous polymer for preparative separations of biological products.
abstract: A continuous rod of porous poly(glycidy1 methacrylate-co-ethylene dimethacrylate) has been prepared by a free radical polymerization within the confines of a 16-mm-i.d. glass column. The epoxide groups of the rod have been modified in situ by their reaction with diethylamine to afford the ionizable weak base 1-N,N-diethylamino-2-hydroxypropyl functionalities that are required for the ion-exchange chromatographic mode. The bimodal pore size distribution curve typical for other molded separation media also prevail for the preparative-size rod. The column has been used successfully for the chromatographic separation of a mixture of standard proteins and yeast enzymes. The column exhibits a dynamic capacity that exceeds 420 mg of bovine serum albumin at a flow velocity of 60 cm/h. (c) 1995 John Wiley & Sons, Inc.
authors.affiliation: Cornell University, Department of Chemistry, Baker Laboratory, lthaca, New York 14853-1301.
authors.forename: F
authors.initials: F
authors.lastname: Svec
authors.forename: J M
authors.initials: JM
authors.lastname: Fréchet