Nucleic acid species related to the satellite RNA of turnip crinkle virus in turnip plants and virus particles.
Multiple RNA species which hybridize to a cloned cDNA probe of the turnip crinkle virus satellite RNA (S-TCV) are found both in virions and in plants infected with virus particles containing S-TCV. At least six of these RNAs appear to be multimeric forms of the satellite RNA based on their migration in denaturing gels. Hybridization to strand-specific probes indicates that most of the RNAs present in both infected cells and encapsidated in the virus particles represent strands of the same polarity as S-TCV. We have detected a small RNA species which is homologous to the satellite RNA that appears in plants inoculated with the gel-purified viral genomic RNA. In addition, we have found homology between the cloned satellite probe and DNA sequences in the genome of the uninfected plant.
Altenbach
S B
SB
Department of Biology, C-016, University of California, San Diego, La Jolla, California 92093, USA.
Howell
S H
SH