title: Immune-driven recombination and loss of control after HIV superinfection.
abstract: After acute HIV infection, CD8(+) T cells are able to control viral replication to a set point. This control is often lost after superinfection, although the mechanism behind this remains unclear. In this study, we illustrate in an HLA-B27(+) subject that loss of viral control after HIV superinfection coincides with rapid recombination events within two narrow regions of Gag and Env. Screening for CD8(+) T cell responses revealed that each of these recombination sites (approximately 50 aa) encompassed distinct regions containing two immunodominant CD8 epitopes (B27-KK10 in Gag and Cw1-CL9 in Env). Viral escape and the subsequent development of variant-specific de novo CD8(+) T cell responses against both epitopes were illustrative of the significant immune selection pressures exerted by both responses. Comprehensive analysis of the kinetics of CD8 responses and viral evolution indicated that the recombination events quickly facilitated viral escape from both dominant WT- and variant-specific responses. These data suggest that the ability of a superinfecting strain of HIV to overcome preexisting immune control may be related to its ability to rapidly recombine in critical regions under immune selection pressure. These data also support a role for cellular immune pressures in driving the selection of new recombinant forms of HIV.
authors.affiliation: Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Streeck
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.forename: Art F Y
authors.initials: AF
authors.lastname: Poon
authors.forename: Arne
authors.initials: A
authors.lastname: Schneidewind
authors.forename: Adrianne D
authors.initials: AD
authors.lastname: Gladden
authors.forename: Karen A
authors.initials: KA
authors.lastname: Power
authors.forename: Demetre
authors.initials: D
authors.lastname: Daskalakis
authors.forename: Suzane
authors.initials: S
authors.lastname: Bazner
authors.forename: Rosario
authors.initials: R
authors.lastname: Zuniga
authors.forename: Christian
authors.initials: C
authors.lastname: Brander
authors.forename: Eric S
authors.initials: ES
authors.lastname: Rosenberg
authors.forename: Simon D W
authors.initials: SD
authors.lastname: Frost
authors.forename: Marcus
authors.initials: M
authors.lastname: Altfeld
authors.forename: Todd M
authors.initials: TM
authors.lastname: Allen
mesh_terms: D001483:Base Sequence; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology / Q000821:virology; D016022:Case-Control Studies; D017931:DNA Primers / Q000235:genetics; D004279:DNA, Viral / Q000235:genetics; D015752:Genes, env; D015750:Genes, gag; D014644:Genetic Variation; D006678:HIV / Q000235:genetics* / Q000276:immunology*; D015658:HIV Infections / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D015796:HLA-B27 Antigen / Q000235:genetics / Q000276:immunology; D006801:Humans; D016056:Immunodominant Epitopes / Q000235:genetics; D011995:Recombination, Genetic; D012641:Selection, Genetic; D015163:Superinfection / Q000235:genetics / Q000276:immunology* / Q000821:virology*; D014766:Viremia / Q000235:genetics / Q000276:immunology / Q000821:virology; D014779:Virus Replication / Q000235:genetics / Q000276:immunology
chemical_list: D017931:DNA Primers; D004279:DNA, Viral; D015796:HLA-B27 Antigen; D016056:Immunodominant Epitopes