Amines and oximes derived from deoxybenzoins as Helicobacter pylori urease inhibitors.
Twenty amines and oximes from deoxybenzoins were prepared and evaluated for their inhibitory activity against Helicobacter pylori urease. Among these compounds, high inhibitory activities were observed in amines and oximes, especially amines 1b (IC(50)=0.011 mM) and 6b (IC(50)=0.047 mM) exhibited good in vitro activities, and were comparable to acetohydroxamic acid (AHA). The hydroxyl groups on deoxybenzoin skeleton may be responsible for the inhibitory activity and coordinate with the nickel (active site) on enzyme. A direct interaction may exist between the OH group of hydroxylamines or NH group of amines and His alpha323 of H. pylori urease, which is on the flap of the enzyme active site.
Li
Huan-Qiu
HQ
Institute of Functional Biomolecules, State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
Xiao
Zhu-Ping
ZP
Yin-Luo
Yan
Tao
T
Lv
Peng-Cheng
PC
Zhu
Hai-Liang
HL
D000588:Amines / Q000737:chemistry* / Q000494:pharmacology; D001573:Benzoin / Q000031:analogs & derivatives; D020134:Catalytic Domain; D016480:Helicobacter pylori / Q000187:drug effects / Q000201:enzymology*; D006877:Hydroxamic Acids; D020128:Inhibitory Concentration 50; D010091:Oximes / Q000737:chemistry* / Q000494:pharmacology; D013329:Structure-Activity Relationship; D014510:Urease / Q000037:antagonists & inhibitors*
D000588:Amines; D006877:Hydroxamic Acids; D010091:Oximes; C006358:acetohydroxamic acid; D014510:Urease; C492807:deoxybenzoin; D001573:Benzoin