title: Oral immunization with recombinant lactobacillus plantarum induces a protective immune response in mice with Lyme disease.
abstract: Mucosal immunization is advantageous over other routes of antigen delivery because it can induce both mucosal and systemic immune responses. Our goal was to develop a mucosal delivery vehicle based on bacteria generally regarded as safe, such as Lactobacillus spp. In this study, we used the Lyme disease mouse model as a proof of concept. We demonstrate that an oral vaccine based on live recombinant Lactobacillus plantarum protects mice from tick-transmitted Borrelia burgdorferi infection. Our method of expressing vaccine antigens in L. plantarum induces both systemic and mucosal immunity after oral administration. This platform technology can be applied to design oral vaccine delivery vehicles against several microbial pathogens.
authors.affiliation: Department of Microbiology and Immunology, New York Medical College, Valhalla, New York,USA.
authors.forename: Beatriz
authors.initials: B
authors.lastname: del Rio
authors.forename: Raymond J
authors.initials: RJ
authors.lastname: Dattwyler
authors.forename: Miguel
authors.initials: M
authors.lastname: Aroso
authors.forename: Vera
authors.initials: V
authors.lastname: Neves
authors.forename: Luciana
authors.initials: L
authors.lastname: Meirelles
authors.forename: Jos F M L
authors.initials: JF
authors.lastname: Seegers
authors.forename: Maria
authors.initials: M
authors.lastname: Gomes-Solecki
mesh_terms: D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D001769:Blood / Q000382:microbiology; D025065:Borrelia burgdorferi / Q000235:genetics* / Q000276:immunology; D004199:Disease Vectors; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry; D005260:Female; D006321:Heart / Q000382:microbiology; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D018884:Ixodes; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000276:immunology*; D008193:Lyme Disease / Q000517:prevention & control*; D022123:Lyme Disease Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D001743:Urinary Bladder / Q000382:microbiology
chemical_list: D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022123:Lyme Disease Vaccines