title: Development of a stable isotope dilution assay for the quantitation of glycidamide and its application to foods and model systems.
abstract: On the basis of a stable isotope dilution assay and derivatization with 2-mercaptobenzoic acid, the presence of the carcinogenic glycidamide ( 2) in processed foods was verified for the first time. Using (13)C-labeled 2 as the internal standard and the formation of the thioether derivatives, a new stable isotope dilution assay for the quantitation of 2 was developed. Application of the method on several potato samples revealed amounts between 0.3 and 1.5 mug/kg depending on the processing conditions. In a model experiment, the formation of 2 by an epoxidation of the double bond in acrylamide, that is, by a reaction with linoleic acid hydroperoxides, was established. This result was in good agreement with data showing that French fries processed in sunflower oil, which is high in linoleic acid, contained more 2 as compared to fries prepared in coconut oil. The derivatization procedure allows the simultaneous quantitation of acrylamide and glycidamide in foods.
authors.affiliation: Chair for Food Chemistry, Technical University of Munich, and German Research Center for Food Chemistry, Lichtenbergstrasse 4, D-85748 Garching, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Granvogl
authors.forename: Peter
authors.initials: P
authors.lastname: Koehler
authors.forename: Larissa
authors.initials: L
authors.lastname: Latzer
authors.forename: Peter
authors.initials: P
authors.lastname: Schieberle
mesh_terms: D020106:Acrylamide / Q000032:analysis / Q000737:chemistry; D002250:Carbon Radioisotopes; D000074263:Coconut Oil; D004852:Epoxy Compounds / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling / Q000379:methods; D007201:Indicator Dilution Techniques; D019787:Linoleic Acid / Q000737:chemistry; D010938:Plant Oils / Q000737:chemistry; D011198:Solanum tuberosum / Q000737:chemistry; D000074242:Sunflower Oil
chemical_list: D002250:Carbon Radioisotopes; D004852:Epoxy Compounds; D010938:Plant Oils; D000074242:Sunflower Oil; D020106:Acrylamide; C071834:glycidamide; D019787:Linoleic Acid; D000074263:Coconut Oil