[The study of Xuanshen on ventricular remodeling and cardiac endothelin-1 expression in rats treated with pressure-overload].
OBJECTIVE
To investigate the influence of Xuanshen on cardiac endothelin-1 expression, ventricular remodeling and its mechanism in rats treated with pressure-overload.


METHODS
The ventricular remodeling model was induced by abdominal aortic stenosis in rats. Meanwhile, sham-operated rats were established as the control group. 8 weeks after drug interference, heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), left ventricular weight and heart weight index (LVWI and HWI), the activity of superoxide dismutase (SOD), cardiac endothelin-1 concentration and its gene expression were determined.


RESULTS
Compared with those of sham-operated rats, the HR, SBP, DBP, LVWI and HWI of the model rats were increased significantly. The activity of SOD decreased, the concentration of cardiac endothelin-1 and its gene expression increased. In groups treated with Xuanshen, the HR, SBP, DBP, LVWI and HWI declined and the activity of SOD was improved. Moreover, the concentration of cardiac endothelin-1 and its gene expression decreased.


CONCLUSIONS
Pressure-overload may induce oxidative stress and over-expression of cardiac endothelin-1. Xuanshen can inhibit ventricular remodeling. The mechanism may be related to the inhibition of oxidative stress and down regulation of endothelin-1 expression.
Gu
Wei-Liang
WL
Department of Pharmacology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Chen
Chang-Xun
CX
D000818:Animals; D001794:Blood Pressure / Q000187:drug effects; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D019332:Endothelin-1 / Q000235:genetics / Q000378:metabolism*; D015870:Gene Expression; D006339:Heart Rate / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D010946:Plants, Medicinal / Q000737:chemistry; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D032266:Scrophularia / Q000737:chemistry*; D013482:Superoxide Dismutase / Q000378:metabolism; D020257:Ventricular Remodeling / Q000187:drug effects*
D004365:Drugs, Chinese Herbal; D019332:Endothelin-1; D012333:RNA, Messenger; D013482:Superoxide Dismutase