Expression of nerve growth factor and its receptors trkA and p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters.
Characteristic daily increases in the plasma levels of luteinizing hormone (LH) are present every afternoon during lactation in golden hamsters. The objective of this study was to investigate the effect of the diurnal rhythm of increases in LH on expression of nerve growth factor (NGF), its receptors trkA and p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters. Both lactating and non-lactating groups of postpartum golden hamsters were used in this study. The expression of NGF, its receptors trkA and p75 and inhibin alpha-subunit were determined by immunohistochemistry. Positive staining of NGF, trkA and p75 was found in the interstitial cells of the lactating group, and no immunoreactivity for NGF, trkA or p75 was observed in the ovarian interstitial cells of the non-lactating group. In addition, immunostaining of inhibin alpha-subunit was also observed in the interstitial cells of the lactating group but not in those of the non-lactating group. Immunostaining of the inhibin/activin beta(A)- and beta(B)-subunits was observed in the granulosa cells of antral follicles, but not in the interstitial cells of the lactating and non-lactating animals. These results suggest that the diurnal rhythm increases in LH can induce expression of NGF, trkA, p75 and inhibin alpha-subunit in the ovarian interstitial cells of lactating golden hamsters and that NGF, its receptors trkA and p75 and inhibin alpha-subunit may have the capacity for autocrine or paracrine modulation of interstitial cell differentiation in golden hamsters.
Weng
Qiang
Q
College of Biological Science and Technology, Beijing Forestry University, Beijing, China. weng3@msn.com
Shi
Zhanquan
Z
Kawaguchi
Maiko
M
Watanabe
Gen
G
Taya
Kazuyoshi
K
D000818:Animals; D000831:Animals, Newborn; D000833:Animals, Suckling; D006224:Cricetinae; D005260:Female; D007265:Inhibins / Q000378:metabolism*; D007774:Lactation / Q000378:metabolism*; D008426:Maternal Deprivation; D008647:Mesocricetus; D020932:Nerve Growth Factor / Q000378:metabolism*; D006080:Ovarian Follicle / Q000502:physiology; D049590:Postpartum Period / Q000378:metabolism / Q000502:physiology; D020800:Receptor, Nerve Growth Factor / Q000378:metabolism*; D020917:Receptor, trkA / Q000378:metabolism*; D013799:Theca Cells / Q000378:metabolism*
D020800:Receptor, Nerve Growth Factor; C094377:inhibin-alpha subunit; D007265:Inhibins; D020932:Nerve Growth Factor; D020917:Receptor, trkA