title: [Influence of microwave radiation on synapsin I expression in PC12 cells and its mechanism].
abstract: OBJECTIVE
  To investigate the effect of microwave radiation on expression and phosphorylation of synapsin I and to discover the mechanism by research on the change of expression of BDNF and its receptor, TrkB.
  
  
  METHODS
  PC12 cells were exposed to microwave with average power density being 30 mW/cm(2). HPLC was used to detect the release of amino acids; RT-PCR, Western blot and immunocytochemistry were used to detect the expressions of synapsin I, BDNF and TrkB; immune co-precipitation was used to study the interaction of BDNF and TrkB.
  
  
  RESULTS
  It resulted in the decrease of the release of Asp, Glu, GABA and Gly at 1 h (P<0.01) after radiation. Protein of synapsin I was decreased in 9 h-2 d (P<0.01 or P<0.05); its mRNA was decreased in 3-9 h and increased at 1 d (P<0.01 or P<0.05); its phosphorylation was decreased at 3 h, increased at 1 d, and decreased at 2 d again (P<0.01 or P<0.05) after radiation. Protein of BDNF was decreased at 3 h and increased in 1-2 d (P<0.01 or P<0.05); its mRNA were decreased in 3-9 h, increased at 1d, and decreased at 2 d again (P<0.01 or P<0.05) after radiation. Protein of TrkB was increased in 3 h-1 d (P<0.01 or P<0.05); its mRNA decreased at 3 h and 2 d (P<0.01) after radiation. The interaction between BDNF and TrkB was increased in 3-9 h, but decreased in 1-2 d (P<0.01 or P<0.05) after radiation.
  
  
  CONCLUSIONS
  Microwave radiation can induce the decrease of the release of amino acids and the expression and phosphorylation of synapsin I, and the abnormality of expressions and interaction of BDNF and TrkB in PC12 cells. The factors might play a role in the injury and repair of information transmission in PC12 cells.
authors.affiliation: Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
authors.forename: Li-Feng
authors.initials: LF
authors.lastname: Wang
authors.forename: Rui-Yun
authors.initials: RY
authors.lastname: Peng
authors.forename: Xiang-Jun
authors.initials: XJ
authors.lastname: Hu
authors.forename: Ya-Bing
authors.initials: YB
authors.lastname: Gao
authors.forename: Shui-Ming
authors.initials: SM
authors.lastname: Wang
authors.forename: Rong-Lian
authors.initials: RL
authors.lastname: Gao
authors.forename: Xin-Ping
authors.initials: XP
authors.lastname: Xu
authors.forename: Zhen-Tao
authors.initials: ZT
authors.lastname: Su
authors.forename: Ji
authors.initials: J
authors.lastname: Dong
mesh_terms: D000818:Animals; D015153:Blotting, Western; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D007150:Immunohistochemistry; D047468:Immunoprecipitation; D008872:Microwaves; D016716:PC12 Cells; D011485:Protein Binding / Q000187:drug effects / Q000235:genetics; D051381:Rats; D020813:Receptor, trkB / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D016704:Synapsins / Q000235:genetics / Q000378:metabolism*
chemical_list: D019208:Brain-Derived Neurotrophic Factor; D016704:Synapsins; D020813:Receptor, trkB