title: A real-time polymerase chain reaction for the detection of Streptococcus pneumoniae in blood using a mouse model: a potential new "gold standard".
abstract: Better diagnostics for pneumococcal disease are urgently needed. In a murine model, real-time polymerase chain reaction was superior to conventional culture in detecting pneumococcus in blood, particularly in early disease and after antibiotic administration, and could distinguish between commensalism and infection.
authors.affiliation: Meningitis and Vaccine-Preventable Diseases Branch, Immunology Laboratories, Division of Bacterial Diseases, Centers for Diseases Control and Prevention, Atlanta, GA 30333, USA. nadine.rouphael@gmail.com
authors.forename: Nadine G
authors.initials: NG
authors.lastname: Rouphael
authors.forename: Nikkol
authors.initials: N
authors.lastname: Atwell-Melnick
authors.forename: Dana
authors.initials: D
authors.lastname: Longo
authors.forename: Melissa
authors.initials: M
authors.lastname: Whaley
authors.forename: George M
authors.initials: GM
authors.lastname: Carlone
authors.forename: Jackie S
authors.initials: JS
authors.lastname: Sampson
authors.forename: Edwin W
authors.initials: EW
authors.lastname: Ades
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D016470:Bacteremia / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D001769:Blood / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004195:Disease Models, Animal; D051379:Mice; D011008:Pneumococcal Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial