Fine mapping of the rice Bph1 gene, which confers resistance to the brown planthopper (Nilaparvata lugens stal), and development of STS markers for marker-assisted selection.
The brown planthopper (BPH) is a major insect pest in rice, and damages these plants by sucking phloem-sap and transmitting viral diseases. Many BPH resistance genes have been identified in indica varieties and wild rice accessions, but none has yet been cloned. In the present study we report fine mapping of the region containing the Bph1 locus, which enabled us to perform marker-aided selection (MAS). We used 273 F8 recombinant inbred lines (RILs) derived from a cross between Cheongcheongbyeo, an indica type variety harboring Bph1 from Mudgo, and Hwayeongbyeo, a BPH susceptible japonica variety. By random amplification of polymorphic DNA (RAPD) analysis using 656 random 10-mer primers, three RAPD markers (OPH09, OPA10 and OPA15) linked to Bph1 were identified and converted to SCAR (sequence characterized amplified region) markers. These markers were found to be contained in two BAC clones derived from chromosome 12: OPH09 on OSJNBa0011B18, and both OPA10 and OPA15 on OSJNBa0040E10. By sequence analysis of ten additional BAC clones evenly distributed between OSJNBa0011B18 and OSJNBa0040E10, we developed 15 STS markers. Of these, pBPH4 and pBPH14 flanked Bph1 at distances of 0.2 cM and 0.8 cM, respectively. The STS markers pBPH9, pBPH19, pBPH20, and pBPH21 co-segregated with Bph1. These markers were shown to be very useful for marker-assisted selection (MAS) in breeding populations of 32 F6 RILs from a cross between Andabyeo and IR71190, and 32 F5 RILs from a cross between Andabyeo and Suwon452.
Cha
Young-Soon
YS
Cell and Genetics Division, National Institute of Agricultural Biotechnology, Suwon 441-707, Korea.
Ji
Hyeonso
H
Yun
Doh-Won
DW
Ahn
Byoung-Ohg
BO
Lee
Myung Chul
MC
Suh
Seok-Cheol
SC
Lee
Chun Seok
CS
Ahn
Eok Keun
EK
Jeon
Yong-Hee
YH
Jin
Il-Doo
ID
Sohn
Jae-Keun
JK
Koh
Hee-Jong
HJ
Eun
Moo-Young
MY
D000818:Animals; D002874:Chromosome Mapping / Q000379:methods*; D017343:Genes, Plant / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics*; D006430:Hemiptera; D012275:Oryza / Q000235:genetics*; D010935:Plant Diseases / Q000469:parasitology*; D016324:Sequence Tagged Sites
D005819:Genetic Markers