Induction of a nonoccluded baculovirus persistently infecting Heliothis zea cells by Heliothis armigera and Trichoplusia ni nuclear polyhedrosis viruses.
A nonoccluded singly enveloped baculovirus (baculovirus X) persistently infects Heliothis zea (IMC-HZ-1) cells in culture. Singly enveloped nuclear polyhedrosis viruses from H. zea and Heliothis armigera, and multiply enveloped nuclear polyhedrosis viruses from Trichoplusia ni, Spodoptera frugiperda, and Spodoptera littoralis were all found to induce baculovirus X. Experiments are reported which use metabolic inhibitors and inactivated inducing virus to show that it is probable that a structural component of the virus, most likely a protein, is responsible for inducing baculovirus X. The persistent virus is induced to replicate by uv-inactivated virus but not by heat-inactivated inducing virus. The virus is not induced to replicate by a number of metabolic inhibitors in the absence of an inducing virus. Inhibition of transcription and translation prevents the induction of the persistent virus by an inducing virus. Inhibition of DNA replication has no effect on the induction of the virus. This suggests that the persistent virus genome is present in abundance in all cells.
Kelly
D C
DC
Natural Environment Research Council, Institute of Virology, 5 South Parks Road, Oxford OX1 3UB, United Kingdom.
Lescott
T
T
Ayres
M D
MD
Carey
D
D
Coutts
A
A
Harrap
K A
KA