Aberrant expression of B203.13 antigen in acute lymphoid leukemia of B-cell origin.
The B203.13 monoclonal antibody was developed by immunizing mice with the B/monocyte biphenotypic cell line B1b. During normal hematopoiesis B203.13 is expressed on a fraction of CD34+ cells, while on mature cells it is only present on B-lymphocytes. We tested this antibody as a marker of childhood B-acute lymphoblastic leukemia (B-ALL). Bone marrow aspirates from 139 cases of early B-ALL and 25 controls were studied. About 40% of the B-ALL patients expressed B203.13. In these patients, B203.13 was constantly co-expressed with CD10, but never co-expressed with CD20, contrary to the controls. The CD10(+)/B203.13(+) phenotype was specific to B-ALL, since CD10(+)/CD20(+) cells from common acute lymphoblastic leukemia (c-ALL) did not express B203.13. We concluded that the use of B203.13 in association with CD10 and CD20 provides meaningful information for distinguishing normal residual B-cells from leukemic B-lymphoblasts and that recurrence of a CD10(+)/B203.13(+) phenotype after transplantation may be a very early relapse indicator of early B-acute lymphoblastic leukemia.
Gobbi
Giuliana
G
Department of Anatomy, Pharmacology and Forensic Medicine, Human Anatomy Section, University of Parma, Ospedale Maggiore, I-43100 Parma, Italy.
Mirandola
Prisco
P
Malinverno
Chiara
C
Sponzilli
Ivonne
I
Carubbi
Cecilia
C
Ricci
Francesca
F
Binazzi
Roberto
R
Basso
Giuseppe
G
Giuliani-Piccari
Gabriella
G
Ramazzotti
Giulia
G
Pasquantonio
Guido
G
Cocco
Lucio
L
Vitale
Marco
M
D000293:Adolescent; D000328:Adult; D018951:Antigens, CD20 / Q000096:biosynthesis / Q000276:immunology; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D008297:Male; D015260:Neprilysin / Q000096:biosynthesis / Q000276:immunology; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000378:metabolism*
D018951:Antigens, CD20; D000951:Antigens, Neoplasm; D015260:Neprilysin