Production of a maternal-zygotic medaka mutant using hybrid sterility.
Taking advantage of the characteristics that make hybrids between Japanese and Chinese medaka grow well, albeit sterile, we have developed a method of germ-line replacement in which these hybrids are used as hosts for the production of a maternal-zygotic mutant. The protocol is described herein.
Shimada
Atsuko
A
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo 7-3-1, Tokyo 113-0033, Japan.
Takeda
Hiroyuki
H
D000818:Animals; D030801:Animals, Genetically Modified; D017690:Cell Transplantation / Q000379:methods; D015509:Developmental Biology / Q000379:methods; D005260:Female; D005821:Genetic Techniques; D005838:Genotype; D005854:Germ Cells / Q000166:cytology; D018095:Germ-Line Mutation; D006579:Heterozygote; D008297:Male; D051379:Mice; D009154:Mutation; D009990:Oryzias / Q000235:genetics*; D015053:Zygote