CD4 independent binding of HIV gp120 to mannose receptor on human spermatozoa.
OBJECTIVE
To characterize the CD4-independent HIV-binding protein of 160 kDa on human spermatozoa.


METHODS
The N-terminal amino acid sequence of the 160 kDa protein and its peptide obtained by tryptic digestion were determined. Polymerase chain reaction amplification of human testicular cDNA was performed using degenerate primers corresponding to peptide sequences of the 160 kDa protein. Localization of 160 kDa protein on sperm was performed using fluorescently labeled gp120, followed by inhibition experiments using antagonists to determine the specificity.


RESULTS
The partial cDNA sequence of the 160 kDa protein demonstrated 99% identity with human macrophage mannose receptor. Sequence of testicular mannose receptor was obtained and exhibited 99% identity with that of macrophage mannose receptor. Furthermore, mannose receptor protein from sperm extract was found to have a molecular weight of 160 kDa, congruent with that of 160 kDa HIV-binding protein. gp120 binding and mannose receptor expression were localized to the equatorial segment in 10% of ejaculated sperm, which increased after capacitation. Mannan at molar excess concentrations completely inhibited gp120 binding to sperm.


CONCLUSIONS
The 160 kDa, CD4-independent HIV-binding sperm protein has been identified as the human mannose receptor protein. The role of mannose receptor in HIV transmission and association with risk of sexual transmission merit further investigation.
Fanibunda
Sashaina E
SE
Department of Biochemistry, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, Maharashtra, India.
Velhal
Shilpa M
SM
Raghavan
Vijaya P
VP
Bandivdekar
Atmaram H
AH
D015704:CD4 Antigens; D018076:DNA, Complementary; D006678:HIV / Q000378:metabolism*; D015699:HIV Envelope Protein gp120 / Q000378:metabolism*; D015658:HIV Infections / Q000378:metabolism / Q000821:virology*; D006801:Humans; D008297:Male; D008358:Mannose / Q000378:metabolism*; D008969:Molecular Sequence Data; D008970:Molecular Weight; D011485:Protein Binding; D015728:Receptors, HIV / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid; D013094:Spermatozoa / Q000737:chemistry* / Q000378:metabolism
D015704:CD4 Antigens; D018076:DNA, Complementary; D015699:HIV Envelope Protein gp120; D015728:Receptors, HIV; D008358:Mannose