Structure of herpes simplex virus DNA: topography of the molecule. I. Absence of circularly permuted sequences.
Only linear structures were produced by re-annealing denatured herpes simplex type 1 virus (HSV)-DNA molecules, while T4-DNA molecules (known to be circularly permuted and therefore used as controls in the parallel tests) formed circles. These results suggested that HSV-DNA is a nonpermuted collection of sequences. Unfortunately, most of the linear structures observed after re-annealing were not full-length duplexes, thus making this test not quite satisfactory. Therefore another permutation test, the central region deletion experiment, was employed. Its results indicated that short fragments liberated from the ends of HSV-DNA by enzymatic treatment contained only a portion of the sequences present in the complete molecules. On the other hand, the presence of apparently all sequences was found in the short end-fragments of the control T4-DNA. These data also provide evidence that HSV-DNA molecules are not formed by circularly permuted collections of sequencies.
Hirsch
I
I
Department of Experimental Virology, Institute of Sera and Vaccines, Prague, Czechoslovakia.
Reischig
J
J
Roubal
J
J
Vonka
V
V