Cryopreserved human bone marrow mononuclear cells as a source of mesenchymal stromal cells: application in osteoporosis research.
BACKGROUND
Mesenchymal stromal cells (MSC) are an invaluable tool for research and therapeutic application regarding degenerative diseases such as osteoporosis.


METHODS
Human MSC from cryopreserved mononuclear (c-MSC) cell populations were isolated from bone marrow (BM) and compared with MSC isolated directly from the same BM for immunophenotype, differentiation capacity and Parathormone (PTH) response.


RESULTS
c-MSC showed a similar immunophenotype, division and differentiation capacity as standard MSC obtained from the same BM. This capacity was maintained during various culture-growing passages. Treatment with PTH(1-34) from days 6 to 24, after c-MSC induction to osteoblasts and adipocytes, had no significant effect on osteoblastogenesis yet inhibited adipogenesis. This effect was similar in MSC from the same BM.


CONCLUSIONS
We propose cryopreservation of mononuclear cells obtained from BM as a simple and convenient means for routine storage of MSC to be used for therapeutic and research applications.
Casado-Díaz
A
A
SANYRES XXI (Grupo PRASA), Córdoba, Spain.
Santiago-Mora
R
R
Jiménez
R
R
Caballero-Villarraso
J
J
Herrera
C
C
Torres
A
A
Dorado
G
G
Quesada-Gómez
J M
JM
D000293:Adolescent; D000328:Adult; D001853:Bone Marrow / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D015925:Cryopreservation; D005260:Female; D006801:Humans; D016130:Immunophenotyping; D007963:Leukocytes, Mononuclear; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D010024:Osteoporosis / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D010281:Parathyroid Hormone / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism
D010281:Parathyroid Hormone