title: Polymorphism in the ITS region of ribosomal DNA of Cochliobolus sativus isolates differing in xylanase production.
abstract: The restriction of PCR-amplified internal transcribed spacers (ITS) ofribosomal DNA was used to confirm the genetic variation among 22 isolates of Cochliobolus sativus differing in their xylanase production. Results show a high level of diversity of ITS-RFLP markers among the isolates. The molecular parameter used showed that C. sativus isolates reside in three phylogenetic groups. There was observed the resolution between clustering of isolates and their xylanase production level.
authors.affiliation: Department of Molecular Biology and Biotechnology, AECS, Damascus, Syria.
authors.forename: Yasser
authors.initials: Y
authors.lastname: Bakri
authors.forename: Mohammed
authors.initials: M
authors.lastname: Jawhar
authors.forename: Mohammed Imad Eddin
authors.initials: MI
authors.lastname: Arabi
mesh_terms: D001203:Ascomycota / Q000145:classification / Q000201:enzymology / Q000235:genetics* / Q000302:isolation & purification; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D043364:Endo-1,4-beta Xylanases / Q000096:biosynthesis*; D014644:Genetic Variation; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D013593:Syria
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D043364:Endo-1,4-beta Xylanases