[Estimation of protective effect of lipoic acid in the brain of rats during lipopolysaccharide-induced oxidative stress].
The aim of his study was to assess the effect of lipoic acid (LA) on concentration of protein in the homogenates of the cerebral cortex, the hypothalamus and the cerebellum in the early stage of oxidative stress.


METHODS
Experiments were performed on male rats Wistar. The animals were divided into four groups consisting of eight animals each. Control group received saline alone into the tail vein (i.v.). The second group received LA (60 mg/kg b.w., iv.). The third group received only lipopolysaccharide (LPS) (30 mg/kg b.w., iv.). The fourth group received LPS (30 mg/kg b.w., iv.) and after 0.5 h received LA (60 mg/kg b.w., iv.). In the homogenates of the cerebral cortex, the hypothalamus and the cerebellum was measured concentration of protein by Lowry method.


RESULTS
Treatment of LPS-injected rats with LA caused increase concentration of protein in the brain structures (p < 0.05).


CONCLUSIONS
The early administration of LA a significant suppressed symptoms of oxidative stress-induced LPS, expressed as a increase in concentration of protein in the study brain structures.
Goraca
Anna
A
Medical University of Lódź, Chair of Experimental and Clinical Physiology, Department of Cardiovascular Physiology, Poland. agoraca@zdn.am.lodz.pl
Cieśla
Włodzimierz
W
D000818:Animals; D001921:Brain / Q000378:metabolism; D002531:Cerebellum / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008070:Lipopolysaccharides; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D011506:Proteins / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000378:metabolism / Q000494:pharmacology*
D008070:Lipopolysaccharides; D011506:Proteins; D008063:Thioctic Acid