title: The nucleoprotein and the viral RNA of infectious salmon anemia virus (ISAV) are localized in the nucleolus of infected cells.
abstract: The infectious salmon anemia virus (ISAV), which belongs to the new genus Isavirus of the Orthomyxoviridae family, is an important pathogen of the salmon farming industry. Indirect immunofluorescence assays carried out with monoclonal antibodies specific for the nucleoprotein (NP) reveal differential staining of sub-cellular compartments in infected cells. Particularly interesting was the staining of the nucleolus, which showed co-localization with nucleolin in CHSE-214, EPC and SHK-1 cells infected with ISAV. These results were confirmed by co-immunoprecipitation studies showing an interaction between NP and nucleolin. In addition, in situ hybridization carried out with probes specific for each of the 8 RNA segments of ISAV showed that the genomic as well as the anti-genomic strands were also localized in the nucleolus. These results suggest a role of the nucleolus in the replication and/or in the packaging of the ISAV genome.
authors.affiliation: Grupo Bios S.A., Chile.
authors.forename: Bertsy
authors.initials: B
authors.lastname: Goić
authors.forename: Juanita
authors.initials: J
authors.lastname: Bustamante
authors.forename: Alvaro
authors.initials: A
authors.lastname: Miquel
authors.forename: Marco
authors.initials: M
authors.lastname: Alvarez
authors.forename: M Ines
authors.initials: MI
authors.lastname: Vera
authors.forename: Pablo D T
authors.initials: PD
authors.lastname: Valenzuela
authors.forename: Luis O
authors.initials: LO
authors.lastname: Burzio
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D002466:Cell Nucleolus / Q000737:chemistry*; D019084:Fluorescent Antibody Technique, Indirect; D047468:Immunoprecipitation; D017403:In Situ Hybridization; D046829:Isavirus / Q000502:physiology*; D018613:Microscopy, Confocal; D008969:Molecular Sequence Data; D009698:Nucleoproteins / Q000032:analysis*; D010750:Phosphoproteins / Q000032:analysis; D012367:RNA, Viral / Q000032:analysis*; D016601:RNA-Binding Proteins / Q000032:analysis; D012474:Salmon; D016415:Sequence Alignment; D019065:Virus Assembly; D014779:Virus Replication; D000097796:Nucleolin
chemical_list: D009698:Nucleoproteins; D010750:Phosphoproteins; D012367:RNA, Viral; D016601:RNA-Binding Proteins; D000097796:Nucleolin