title: Candida albicans infection delays duodenal ulcer healing in cysteamine-induced duodenal ulcers in rats.
abstract: A low curability of ulcers infected with Candida has been reported in the literature. The aim of the study reported here was to investigate experimentally whether Candida infection affects the healing of ulcers. Candida albicans (the Candida group) or saline (the control group) was administered intragastrically into rats with a cysteamine-induced duodenal ulcer. The duodenal lesions, vascular endothelial growth factor A (VEGF-A) and proliferating cell nuclear antigen (PCNA) were assessed. On Day 7 post-administration, 70.4% rats of the Candida group had a duodenal ulcer compared with 33.3% in the control group (P < 0.05). The duodenal ulcer in the Candida group was significantly larger and deeper than that in the control group. The number of VEGF-A- and PCNA-positive cells was smaller and the area of VEGF-A expression was lower in the Candida group. Using a rat model, we have demonstrated that Candida infection can delay the wound healing process of duodenal ulcers by means of a low expression of VEGF-A and PCNA.
authors.affiliation: Department of Surgery, Keio University School of Medicine, Shinjuku-ku, Tokyo, 160-8582, Japan.
authors.forename: Longxue
authors.initials: L
authors.lastname: Jin
authors.forename: Masashi
authors.initials: M
authors.lastname: Yoshida
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nakamura
authors.forename: Hideki
authors.initials: H
authors.lastname: Ishikawa
authors.forename: Go
authors.initials: G
authors.lastname: Wakabayashi
authors.forename: Minoru
authors.initials: M
authors.lastname: Tanabe
authors.forename: Shigeyuki
authors.initials: S
authors.lastname: Kawachi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Shinoda
authors.forename: Yoshiro
authors.initials: Y
authors.lastname: Saikawa
authors.forename: Norihito
authors.initials: N
authors.lastname: Wada
authors.forename: Kaori
authors.initials: K
authors.lastname: Kameyama
authors.forename: Koichiro
authors.initials: K
authors.lastname: Kumai
authors.forename: Tetsuro
authors.initials: T
authors.lastname: Kubota
authors.forename: Katsuko
authors.initials: K
authors.lastname: Sano
authors.forename: Keisuke
authors.initials: K
authors.lastname: Nagao
authors.forename: Masayuki
authors.initials: M
authors.lastname: Amagai
authors.forename: Yuko
authors.initials: Y
authors.lastname: Kitagawa
authors.forename: Masaki
authors.initials: M
authors.lastname: Kitajima
mesh_terms: D000818:Animals; D002176:Candida albicans / Q000472:pathogenicity*; D002177:Candidiasis / Q000150:complications*; D003543:Cysteamine; D004195:Disease Models, Animal; D004381:Duodenal Ulcer / Q000139:chemically induced / Q000382:microbiology* / Q000503:physiopathology*; D004386:Duodenum / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
chemical_list: D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; D003543:Cysteamine