title: Molecular characterisation of Mycobacterium bovis isolated from cattle slaughtered at the Bamako abattoir in Mali.
abstract: BACKGROUND
  Mali is one of the most important livestock producers of the Sahel region of Africa. A high frequency of bovine tuberculosis (BTB) has been reported but surveillance and control schemes are restricted to abattoir inspections only. The objective of this study was to conduct, for the first time, molecular characterisation of Mycobacterium bovis strains isolated from cattle slaughtered at the Bamako abattoir. Of 3330 animals screened only 60 exhibited gross visible lesions. From these animals, twenty strains of M. bovis were isolated and characterised by spoligotyping.
  
  
  RESULTS
  Organ lesions typical of BTB were most often detected in the liver, followed by the lung and the peritoneum. M. bovis was isolated from 20 animals and 7 different spoligotypes were observed among these 20 strains; three of the patterns had not been previously reported. Spoligotype patterns from thirteen of the strains lacked spacer 30, a characteristic common in strains of M. bovis found in Chad, Cameroon and Nigeria. However, unlike the other three Central African countries, the majority of spoligotype patterns observed in Mali also lacked spacer 6. Of the remaining seven strains, six had spoligotype patterns identical to strains commonly isolated in France and Spain.
  
  
  CONCLUSIONS
  Two groups of M. bovis were detected in cattle slaughtered at the Bamako abattoir. The spoligotype pattern of the first group has similarities to strains previously observed in Chad, Cameroon and Nigeria. The additional absence of spacer 6 in the majority of these strains suggests a Mali specific clone. The spoligotype patterns of the remaining strains suggest that they may have been of European origin.
authors.affiliation: Swiss Tropical Institute, Basel, Switzerland. borna.mueller@unibas.ch
authors.forename: Borna
authors.initials: B
authors.lastname: Müller
authors.forename: Benjamin
authors.initials: B
authors.lastname: Steiner
authors.forename: Bassirou
authors.initials: B
authors.lastname: Bonfoh
authors.forename: Adama
authors.initials: A
authors.lastname: Fané
authors.forename: Noel H
authors.initials: NH
authors.lastname: Smith
authors.forename: Jakob
authors.initials: J
authors.lastname: Zinsstag
mesh_terms: D000003:Abattoirs; D000818:Animals; D015373:Bacterial Typing Techniques; D002417:Cattle; D008302:Mali; D018598:Minisatellite Repeats / Q000235:genetics; D009163:Mycobacterium bovis / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D009841:Oligonucleotides / Q000032:analysis; D014380:Tuberculosis, Bovine / Q000382:microbiology*
chemical_list: D009841:Oligonucleotides