title: Calcitonin gene-related peptide receptor expression in alternatively activated monocytes/macrophages during irreversible pulpitis.
abstract: The purpose of this study was to quantify the percentage and the mean fluorescence intensity of viable alternatively activated monocytes/macrophages (AAMø) CD163+ positive for calcitonin gene-related peptide receptor (CGRPr) within the total AAMø population in human dental pulp. Pulp tissue samples were collected from teeth with a clinical diagnosis of irreversible pulpitis (n = 13), pulps with induced inflammation (n = 13), and normal pulps (n = 13). All samples were labeled to identify positive cells for CGRPr and CD163 using a flow cytometry assay. Results demonstrated that a high percentage of total viable AAMø CD163+ expressed CGRPr on their membranes (72.12% in healthy pulp, 62.20% in irreversible pulpitis, and 58.01% in induced pulpitis). Significant differences were found between mean AAMø CD163+ fluorescence for CGRPr according to pulp condition, being greater in irreversible pulpitis. It can be concluded that AAMø CD163+ are expressed during normal and inflammatory processes, supporting the hypothesis that they could exercise an anti-inflammatory action that could be controlled by CGRP signaling after its binding.
authors.affiliation: Graduate Studies Department, School of Dentistry, Pontificia Universidad Javeriana, Bogotá, Colombia. javiercaviedes@cable.net.co
authors.forename: Javier
authors.initials: J
authors.lastname: Caviedes-Bucheli
authors.forename: Gloria Cristina
authors.initials: GC
authors.lastname: Moreno
authors.forename: María Paula
authors.initials: MP
authors.lastname: López
authors.forename: Ana Milena
authors.initials: AM
authors.lastname: Bermeo-Noguera
authors.forename: Gloriana
authors.initials: G
authors.lastname: Pacheco-Rodríguez
authors.forename: Adriana
authors.initials: A
authors.lastname: Cuellar
authors.forename: Hugo Roberto
authors.initials: HR
authors.lastname: Muñoz
mesh_terms: D000328:Adult; D000704:Analysis of Variance; D015703:Antigens, CD / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000276:immunology; D002478:Cells, Cultured; D003782:Dental Pulp / Q000166:cytology / Q000378:metabolism*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008262:Macrophage Activation; D008264:Macrophages / Q000276:immunology / Q000378:metabolism*; D009000:Monocytes / Q000276:immunology / Q000378:metabolism*; D015213:Neuroimmunomodulation; D011671:Pulpitis / Q000276:immunology / Q000378:metabolism*; D018015:Receptors, Calcitonin Gene-Related Peptide / Q000096:biosynthesis* / Q000502:physiology; D011956:Receptors, Cell Surface / Q000276:immunology; D018709:Statistics, Nonparametric; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D000098902:CD163 Antigen; D018836:Inflammation Mediators; D018015:Receptors, Calcitonin Gene-Related Peptide; D011956:Receptors, Cell Surface