title: Toward a control of lignin and manganese peroxidases hypersecretion by Phanerochaete chrysosporium in agitated vessels: Evidence of the superiority of pneumatic bioreactors on mechanically agitated bioreactors.
abstract: Phanerochaete chrysosporium and cultivated both mechanically agitated and pneumatic bioreactors. In the pneumatic devices, the yields of lignin and manganese peroxidases as well as extracellular protein, were considerably increased as compared with mechanically agitated bioreactors. Lignin peroxidase and manganese peroxidase activities as high as 4500 U x L(-1) and 1812 U x L(-1) respectively, were produced in an airlift bioreactor. By using enzyme markers, the secretion pathway and the respiration were shown to be dramatically activated in pneumatic bioreactors. The general metabolism of the fungus, when cultivated in the conventional fermentors, is oriented toward the synthesis of biomass at the expense of the synthesis of peroxidases. The use of pneumatic devices for the production of extracellular peroxidases by P. chrysosporium, avoids shear effects due to turbine agitator in the conventional fermentors, and provides a good example for the production of shear-sensitive metabolites.
authors.affiliation: Laboratoire de Biotechnologie des Champignons Filamenteux Faculté des Sciences de Luminy, 163 Avenue de Luminy, Case 929, 13288 Marseille cedex 09, France.
authors.forename: P
authors.initials: P
authors.lastname: Bonnarme
authors.forename: M
authors.initials: M
authors.lastname: Delattre
authors.forename: H
authors.initials: H
authors.lastname: Drouet
authors.forename: G
authors.initials: G
authors.lastname: Corrieu
authors.forename: M
authors.initials: M
authors.lastname: Asther