An Efficient reproductive method for Irs2-/- mice with C57BL/6JJcl genetic background.
Efficient reproduction using natural mating and reproduction technology [in vitro fertilization (IVF) and embryo transfer (ET)] was investigated in IRS2 deficient mice with C57BL/6JJcl genetic background (Irs2(-/-) mice) as a typical type 2 diabetes model. From the results using various combinations of Irs2(-/-) and Irs2(-/+) mice, the combination of female Irs2(-/+) x male Irs2(-/-) was found to be more efficient than other combinations. In applications of reproduction technology using IVF and ET, the combination of female Irs2(-/+) x male Irs2(-/-) involves the possibility of Irs2(-/-) production by repeats using female Irs2(-/+) mice. However, reproductive continuity using this combination is difficult because of dependence on human technique and the cost of ET. Therefore, we concluded that Irs2(-/-) mice should be produced by embryo transfer using Irs2(-/-) mice from a colony consisting of female Irs2(-/+) x male Irs2(-/-).
Hashimoto
Haruo
H
Central Institute for Experimental Animals, Kanagawa, Japan.
Eto
Tomoo
T
Kamisako
Tsutomu
T
Hoya
Naoko
N
Hatakeyama
Teruhiko
T
Arai
Toshiro
T
Yokosuka
Makoto
M
Ohnishi
Yasuyuki
Y
Ito
Mamoru
M
Hioki
Kyoji
K
Suzuki
Ryo
R
Ohsugi
Mitsuru
M
Saito
Muneo
M
Ueyama
Yoshito
Y
Yamauchi
Toshimasa
T
Kubota
Naoto
N
Tobe
Kazuyuki
K
Kadowaki
Takashi
T
Tamaoki
Norikazu
N
Nomura
Tatsuji
T
Kosaka
Kinori
K
D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics*