[The study on oral carcinoma-associated fibroblast promoting the proliferation of lingual carcinoma cell line].
OBJECTIVE
The aim of this study was in vitro to investigate the effects of oral carcinoma-associated fibroblasts (CAFs) on the proliferation of lingual carcinoma cells.


METHODS
The interaction model between primary oral CAFs and a lingual carcinoma cell line Tca8113 was established for this study project. The effects of CAFs on the viability and cell cycle of Tca8113 were investigated through morphological observation, MTT assay and flow cytometry.


RESULTS
After oral CAFs interacted with Tca8113 directly, Tca8113 showed the cellular shape changes in morphology; compared with normal fibroblasts (NFs), CAFs enhanced the viability of Tca8113 (P<0.05), and increased the percentage of carcinoma cells in S phase and G2 phase (48.1% vs 40.0%).


CONCLUSIONS
Oral CAFs can promote the proliferation of the lingual carcinoma cell line Tca8113 in vitro, and may play a key role in the progression of oral squamous cell carcinoma (OSCC).
Lin
Jing-Wen
JW
Department of Oral Medicine, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Chen
Qian-Ming
QM
Li
Sheng-Fu
SF
Song
Hui-Yun
HY
Long
Dan
D
Zhou
Hong-Mei
HM
D002294:Carcinoma, Squamous Cell / Q000473:pathology / Q000503:physiopathology; D002450:Cell Communication / Q000502:physiology; D002453:Cell Cycle / Q000502:physiology*; D049109:Cell Proliferation; D048430:Cell Shape / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D018920:Coculture Techniques; D005347:Fibroblasts / Q000166:cytology / Q000502:physiology*; D005434:Flow Cytometry; D009061:Mouth Mucosa / Q000166:cytology; D009062:Mouth Neoplasms / Q000473:pathology / Q000503:physiopathology; D014407:Tumor Cells, Cultured