Lack of serological relationship between the 35K nonstructural protein of alfalfa mosaic virus and the corresponding proteins of three other plant viruses with a tripartite genome.
Alfalfa mosaic virus RNA 3 was translated in the mRNA-dependent rabbit reticulocyte cell-free system. The 35K translational protein was partly purified and used to raise antibodies. The antibodies obtained reacted with the 35K protein directed by AMV RNA 3 but not with the corresponding proteins directed by RNAs from three other viruses with a tripartite genome (tobacco streak, cucumber mosaic, and brome mosaic).
van Tol
R G
RG
Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands.
van Vloten-Doting
L
L