Development of a new assay for evaluation of l-3,4-dihydroxyphenylalanine cytotoxicity in catalase-mutant Escherichia coli.
The present study aimed to assess whether a newly constructed, catalase-deficient Escherichia coli strain that express mammalian catalase gene could be used to identify oxidative stress-generating chemicals. We tested l-3,4-dihydroxyphenylalanine (l-DOPA), a well-known agent that induces reactive oxygen species. We found that l-DOPA exposure reduced the survival of catalase-mutant E. coli in a dose-dependent manner, especially in the strains with lower catalase activities, implying the usefulness of these strains in assessment of oxidative chemicals.
Wang
Da-Hong
DH
Department of Public Health, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700-8558, Japan. dahong@md.okayama-u.ac.jp
Horita
Masako
M
Tsutsui
Ken
K
Sano
Kuniaki
K
Sauriasari
Rani
R
Masuoka
Noriyoshi
N
Takemura
Yoko
Y
Takigawa
Tomoko
T
Takaki
Jiro
J
Ogino
Keiki
K
D001426:Bacterial Proteins / Q000235:genetics*; D001681:Biological Assay / Q000379:methods*; D002374:Catalase / Q000235:genetics*; D004295:Dihydroxyphenylalanine / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D007980:Levodopa / Q000494:pharmacology; D009154:Mutation; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; D007980:Levodopa; D004295:Dihydroxyphenylalanine; D002374:Catalase