Bacillus thuringiensis improved isolation methodology from soil samples.
Bacillus thuringiensis is a sporulating bacterium, which produces parasporal inclusions toxic to insects. Widely used methodologies to isolate Bt from soil consist of a thermal shock treatment followed by selective germination of spores. The results presented here suggest that a preliminary 5 h dry-heat treatment largely enhance the selectivity of these procedures.
Santana
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MA
Departamento de Biología Celular, Universidad Simón Bolívar, Sartenejas, Baruta, Edo. Miranda, Apartado 89000 Caracas, Venezuela. msantana@usb.ve
Moccia-V
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CC
Gillis
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AE
D001413:Bacillus thuringiensis / Q000302:isolation & purification* / Q000502:physiology*; D001431:Bacteriological Techniques; D015169:Colony Count, Microbial; D006358:Hot Temperature; D012988:Soil Microbiology; D013171:Spores, Bacterial / Q000502:physiology