title: Development of SM5-1-conjugated ultrasmall superparamagnetic iron oxide nanoparticles for hepatoma detection.
abstract: Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are maghemite or magnetite nanoparticles currently used as contrast agent in magnetic resonance imaging (MRI). In this study, a targeted contrast agent (SM-USPIO) was prepared by conjugating coprecipitated USPIO to a humanized SM5-1 antibody which can specifically react with human hepatocellular carcinoma (HCC) cells. The binding and internalization of SM-USPIO to the HCC cell line ch-hep-3 was confirmed by flow cytometry and confocal microscopy. Furthermore, SM-USPIO was demonstrated to be able to selectively accumulate in the tumor cells, resulting in a marked decrease of MRI T2-weighted signal intensity. Biodistribution studies demonstrated the efficient accumulation of SM-USPIO in the ch-hep-3 tumor in nude mice. The in vivo study in the ch-hep-3 tumor-bearing nude mice indicated that MRI using the SM-USPIO as contrast agent possessed good diagnostic ability, suggesting that SM-USPIO had the potential to be a promising targeted contrast agent for diagnosis of HCC.
authors.affiliation: International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, New Building 10-11th Floor, Shanghai 200433, People's Republic of China.
authors.forename: Geng
authors.initials: G
authors.lastname: Kou
authors.forename: Shuhui
authors.initials: S
authors.lastname: Wang
authors.forename: Changming
authors.initials: C
authors.lastname: Cheng
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.forename: Bohua
authors.initials: B
authors.lastname: Li
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.forename: Weizhu
authors.initials: W
authors.lastname: Qian
authors.forename: Sheng
authors.initials: S
authors.lastname: Hou
authors.forename: Dapeng
authors.initials: D
authors.lastname: Zhang
authors.forename: Jianxin
authors.initials: J
authors.lastname: Dai
authors.forename: Hongchen
authors.initials: H
authors.lastname: Gu
authors.forename: Yajun
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000911:Antibodies, Monoclonal / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000378:metabolism; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000378:metabolism*; D003911:Dextrans; D005290:Ferric Compounds / Q000378:metabolism; D052203:Ferrosoferric Oxide; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D053758:Nanoparticles; D010087:Oxides / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D003287:Contrast Media; D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D010087:Oxides; C097921:ferumoxtran-10; C000499:ferric oxide; D007501:Iron; D052203:Ferrosoferric Oxide