title: Nitric oxide associated with iNOS expression inhibits acetylcholinesterase activity and induces memory impairment during acute hypobaric hypoxia.
abstract: The mechanisms responsible for cholinergic dysfunction associated learning and memory impairment during hypoxia are not well-understood. However it is known that inflammatory mediators like inducible nitric oxide synthase (iNOS) hamper the functions of cholinergic neurons. In this present experiment we made an effort to study the iNOS expression mediated retrograde and anterograde memory impairment in Balb/c mice following acute hypobaric hypoxia (at an altitude of 23,000ft for 6h) using elevated plus maze and passive avoidance step-through tasks. Our results demonstrated that hypoxia transiently impairs the retrograde memory without affecting the anterograde memory functions, accompanied with a substantial rise in iNOS expression and nitric oxide levels in cerebral cortex on days 2 and 3 post hypoxia. Treatment with aminoguanidine (iNOS inhibitor ), resulted in down-regulation of the iNOS expression, attenuation of the surge of nitric oxide (NO) in cerebral cortex and reversal of retrograde memory impairment due to hypoxia. Moreover the reduced AChE activity and elevated lipid peroxidation in cerebral cortex were evident during post hypoxia re-oxygenation period, which was not observed in the hippocampus. Additionally, NO donor spermine NONOate could inhibit the AChE activity in brain homogenates in a concentration-dependent manner, which further substantiate that nitric oxide produced during post hypoxia re-oxygenation, primarily contributes to the observed inhibition of cortical AChE activity. Based on these experiments we hypothesize that the NO burst as a result of iNOS upregulation during hypoxia interrupts the memory consolidation by altering the cholinergic functions.
authors.affiliation: Dr. B. R. Ambedkar Center for Biomedical Research, University of Delhi, Delhi 110007, India.
authors.forename: M
authors.initials: M
authors.lastname: Udayabanu
authors.forename: D
authors.initials: D
authors.lastname: Kumaran
authors.forename: R Unnikrishnan
authors.initials: RU
authors.lastname: Nair
authors.forename: P
authors.initials: P
authors.lastname: Srinivas
authors.forename: Neeta
authors.initials: N
authors.lastname: Bhagat
authors.forename: R
authors.initials: R
authors.lastname: Aneja
authors.forename: Anju
authors.initials: A
authors.lastname: Katyal
mesh_terms: D000110:Acetylcholinesterase / Q000378:metabolism*; D020324:Amnesia, Anterograde / Q000139:chemically induced / Q000523:psychology; D000648:Amnesia, Retrograde / Q000139:chemically induced / Q000523:psychology; D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects; D015153:Blotting, Western; D002540:Cerebral Cortex / Q000187:drug effects / Q000201:enzymology; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000494:pharmacology; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000096:biosynthesis / Q000235:genetics; D006146:Guanidines / Q000494:pharmacology; D006624:Hippocampus / Q000187:drug effects / Q000201:enzymology; D000860:Hypoxia / Q000201:enzymology* / Q000523:psychology*; D007858:Learning / Q000187:drug effects; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008569:Memory Disorders / Q000139:chemically induced / Q000523:psychology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009569:Nitric Oxide / Q000378:metabolism*; D020030:Nitric Oxide Donors / Q000494:pharmacology; D052247:Nitric Oxide Synthase Type II / Q000037:antagonists & inhibitors* / Q000096:biosynthesis; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: D002800:Cholinesterase Inhibitors; D004791:Enzyme Inhibitors; D006146:Guanidines; D020030:Nitric Oxide Donors; D012333:RNA, Messenger; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; D000110:Acetylcholinesterase; C004479:pimagedine