Replication potentials of vif variant viruses generated from monkey cell-tropic HIV-1 derivative clones NL-DT5/NL-DT5R.
To obtain monkey-tropic viruses that are more closely related to HIV-1 than the original NL-DT5/NL-DT5R clones, we constructed six vif-chimeric and two site-specific vif-mutant viruses, and examined their growth ability. Different from NL-DT5/NL-DT5R, these viruses did not grow in monkey cells. We monitored the capability of the mutants to antagonize monkey APOBEC3G/F by single-cycle infectivity assays. They counteracted poorly or not at all the action of the APOBEC3G/F. Our results have indicated that the native SIVmac Vif is required to overcome the species barrier against HIV-1.
Hatcho
Kazuki
K
Department of Virology, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan.
Kamada
Kazuya
K
Yamashita
Tomoki
T
Adachi
Akio
A
Nomaguchi
Masako
M
D000071480:APOBEC-3G Deaminase; D000818:Animals; D002460:Cell Line; D003001:Cloning, Molecular; D003564:Cytidine Deaminase / Q000235:genetics / Q000378:metabolism; D043525:Cytosine Deaminase / Q000378:metabolism; D016341:Genes, vif; D054303:HIV Reverse Transcriptase / Q000378:metabolism; D015497:HIV-1 / Q000235:genetics / Q000502:physiology*; D000882:Haplorhini / Q000821:virology*; D006801:Humans; D009154:Mutation; D014779:Virus Replication
D054303:HIV Reverse Transcriptase; C492906:APOBEC3F protein, human; D043525:Cytosine Deaminase; D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase