Antihypertensive effect of an angiotensin converting enzyme inhibitory peptide from enzyme modified cheese.
Two angiotensin converting enzyme (ACE)-inhibitory peptides were isolated from enzyme modified cheese (EMC) and their amino acid sequences were identified as Leu-Gln-Pro and Met-Ala-Pro. The EMC was prepared by a combination of Protease N, Umamizyme, and Flavourzyme 500L. Both peptides were derived from beta-casein, f 88-90 and f 102-104, respectively. Met-Ala-Pro showed strong ACE inhibitory activity (IC50=0.8 mum) and antihypertensive activity in spontaneously hypertensive rats (SHR) after single oral administration. The IC50 value of Met-Ala-Pro was not affected by pre-incubation with ACE, suggesting that this peptide was a true ACE-inhibitory peptide. We report here, for the first time antihypertensive peptides from EMC.
Tonouchi
Hidekazu
H
Food Science Institute Division of Research and Development Meiji Dairies Corporation, 540 Naruda, Odawara, Kanagawa 250-0862, Japan. HIDEKAZU_TONOUCHI@MEIJIMILK.COM
Suzuki
Masayuki
M
Uchida
Masayuki
M
Oda
Munehiro
M
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000494:pharmacology*; D001794:Blood Pressure / Q000187:drug effects*; D002611:Cheese; D002851:Chromatography, High Pressure Liquid; D005511:Food Handling / Q000379:methods; D008297:Male; D010455:Peptides / Q000737:chemistry / Q000145:classification / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D013997:Time Factors
D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D010455:Peptides