title: Aberrant expression of B203.13 antigen in acute lymphoid leukemia of B-cell origin.
abstract: The B203.13 monoclonal antibody was developed by immunizing mice with the B/monocyte biphenotypic cell line B1b. During normal hematopoiesis B203.13 is expressed on a fraction of CD34+ cells, while on mature cells it is only present on B-lymphocytes. We tested this antibody as a marker of childhood B-acute lymphoblastic leukemia (B-ALL). Bone marrow aspirates from 139 cases of early B-ALL and 25 controls were studied. About 40% of the B-ALL patients expressed B203.13. In these patients, B203.13 was constantly co-expressed with CD10, but never co-expressed with CD20, contrary to the controls. The CD10(+)/B203.13(+) phenotype was specific to B-ALL, since CD10(+)/CD20(+) cells from common acute lymphoblastic leukemia (c-ALL) did not express B203.13. We concluded that the use of B203.13 in association with CD10 and CD20 provides meaningful information for distinguishing normal residual B-cells from leukemic B-lymphoblasts and that recurrence of a CD10(+)/B203.13(+) phenotype after transplantation may be a very early relapse indicator of early B-acute lymphoblastic leukemia.
authors.affiliation: Department of Anatomy, Pharmacology and Forensic Medicine, Human Anatomy Section, University of Parma, Ospedale Maggiore, I-43100 Parma, Italy.
authors.forename: Giuliana
authors.initials: G
authors.lastname: Gobbi
authors.forename: Prisco
authors.initials: P
authors.lastname: Mirandola
authors.forename: Chiara
authors.initials: C
authors.lastname: Malinverno
authors.forename: Ivonne
authors.initials: I
authors.lastname: Sponzilli
authors.forename: Cecilia
authors.initials: C
authors.lastname: Carubbi
authors.forename: Francesca
authors.initials: F
authors.lastname: Ricci
authors.forename: Roberto
authors.initials: R
authors.lastname: Binazzi
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Basso
authors.forename: Gabriella
authors.initials: G
authors.lastname: Giuliani-Piccari
authors.forename: Giulia
authors.initials: G
authors.lastname: Ramazzotti
authors.forename: Guido
authors.initials: G
authors.lastname: Pasquantonio
authors.forename: Lucio
authors.initials: L
authors.lastname: Cocco
authors.forename: Marco
authors.initials: M
authors.lastname: Vitale
mesh_terms: D000293:Adolescent; D000328:Adult; D018951:Antigens, CD20 / Q000096:biosynthesis / Q000276:immunology; D000951:Antigens, Neoplasm / Q000096:biosynthesis*; D002648:Child; D002675:Child, Preschool; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016130:Immunophenotyping; D007223:Infant; D008297:Male; D015260:Neprilysin / Q000096:biosynthesis / Q000276:immunology; D015452:Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology / Q000378:metabolism*
chemical_list: D018951:Antigens, CD20; D000951:Antigens, Neoplasm; D015260:Neprilysin