title: Human adipose-derived stem cells as future tools in tissue regeneration: osteogenic differentiation and cell-scaffold interaction.
abstract: Tissue engineering is now contributing to new developments in several clinical fields, and mesenchymal stem cells derived from adipose tissue (hASCs) may provide a novel opportunity to replace, repair and promote the regeneration of diseased or damaged musculoskeletal tissue. Our interest was to characterize and differentiate hASCs isolated from twenty-three donors. Proliferation, CFU-F, cytofluorimetric and histochemistry analyses were performed. HASCs differentiate into osteogenic, chondrogenic, and adipogenic lineages, as assessed by tissue-specific markers such as alkaline phosphatase, osteopontin expression and deposition of calcium matrix, lipid-vacuoles formation and Glycosaminoglycans production. We also compared osteo-differentiated hASCs cultured on monolayer and loaded on biomaterials routinely used in the clinic, such as hydroxyapatite, cancellous human bone fragments, deproteinized bovine bone granules, and titanium. Scaffolds loaded with pre-differentiated hASCs do not affect cell proliferation and no cellular toxicity was observed. HASCs tightly adhere to scaffolds and differentiated-hASCs on human bone fragments and bovine bone granules produced, respectively, 3.4- and 2.1-fold more calcified matrix than osteo-differentiated hASCs on monolayer. Moreover, both human and deproteinized bovine bone is able to induce osteogenic differentiation of CTRL-hASCs. Although our in vitro results need to be confirmed in in vivo bone regeneration models, our data suggest that hASCs may be considered suitable biological tools for the screening of innovative scaffolds that would be useful in tissue engineering.
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, University of Milan, Milan, Milan - Italy.
authors.forename: L
authors.initials: L
authors.lastname: De Girolamo
authors.forename: M F
authors.initials: MF
authors.lastname: Sartori
authors.forename: E
authors.initials: E
authors.lastname: Arrigoni
authors.forename: L
authors.initials: L
authors.lastname: Rimondini
authors.forename: W
authors.initials: W
authors.lastname: Albisetti
authors.forename: R L
authors.initials: RL
authors.lastname: Weinstein
authors.forename: A T
authors.initials: AT
authors.lastname: Brini
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials; D002417:Cattle; D002450:Cell Communication; D002454:Cell Differentiation / Q000502:physiology*; D017886:Durapatite; D005347:Fibroblasts / Q000502:physiology; D005434:Flow Cytometry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000502:physiology*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds; D014025:Titanium
chemical_list: D001672:Biocompatible Materials; D017886:Durapatite; D014025:Titanium