title: Purification of recombinant GFP produced by Agrobacterum-mediated transient expression in Nicotiana excelsior.
abstract: Green fluorescent protein (GFP) is commonly used as a reporter protein in a wide range of biological experiments. The efficient protocol of Agrobacterium-mediated transient expression in Nicotiana excelsior was applied for quick preparative production of recombinant GFP. The protein purification scheme has been developed and included ammonium sulfate precipitation and Q-sepharose anion-exchange chromatography. It results in obtaining of a fraction with about 85% GFP homogeneity and the protein yield of about 75%.
authors.affiliation: Institute of Cell Biology and Genetic Engineering, Kyiv, Ukraine.
authors.forename: Y R
authors.initials: YR
authors.lastname: Sindarovska
authors.forename: Y V
authors.initials: YV
authors.lastname: Sheludko
authors.forename: I M
authors.initials: IM
authors.lastname: Gerasymenko
authors.forename: M A
authors.initials: MA
authors.lastname: Bannikova
authors.forename: N V
authors.initials: NV
authors.lastname: Kuchuk
mesh_terms: D004591:Electrophoresis, Polyacrylamide Gel; D005822:Genetic Vectors; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000302:isolation & purification; D011994:Recombinant Proteins / Q000235:genetics / Q000302:isolation & purification; D012231:Rhizobium / Q000235:genetics*; D014026:Nicotiana / Q000235:genetics*
chemical_list: D011994:Recombinant Proteins; D049452:Green Fluorescent Proteins