KAI1/CD82 suppresses hepatocyte growth factor-induced migration of hepatoma cells via upregulation of Sprouty2.
We conducted a study concerning the suppressive mechanism of KAI1/CD82 on hepatoma cell metastasis. Hepatocyte growth factor (HGF) induces the migration of hepatoma cells through activation of cellular sphingosine kinase 1 (SphK1). Adenovirus-mediated gene transfer of KAI1 (Ad-KAI1) downregulates the SphK1 expression and suppresses the HGF-induced migration of SMMC-7721 human hepatocellcular carcinoma cells. Overexpression of KAI1/CD82 significantly elevates Sprouty2 at the protein level. Ablation of Sprouty2 with RNA interference can block the KAI1/CD82-induced suppression of hepatoma cell migration and downregulation of SphK1 expression. It is demonstrated that KAI1/CD82 suppresses HGF-induced migration of hepatoma cells via upregulation of Sprouty2.
Mu
Zhenbin
Z
State Key Laboratory of Cancer Biology and Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.
Wang
Hua
H
Zhang
Jing
J
Li
Qingfang
Q
Wang
Lisheng
L
Guo
Xiaozhong
X
D000256:Adenoviridae / Q000235:genetics; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D004789:Enzyme Activation; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism*; D051861:Kangai-1 Protein / Q000235:genetics / Q000378:metabolism*; D008565:Membrane Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics; D015854:Up-Regulation / Q000187:drug effects*; D000099157:Sphingosine Kinase
D017228:Hepatocyte Growth Factor; D047908:Intracellular Signaling Peptides and Proteins; D051861:Kangai-1 Protein; D008565:Membrane Proteins; D017853:Phosphotransferases (Alcohol Group Acceptor); D034741:RNA, Small Interfering; D000099157:Sphingosine Kinase; C485561:SPRY2 protein, human