Peripheral vesicles in proplastids of barley stripe mosaic virus-infected wheat cells contain double-stranded RNA.
Cytochemical and immunoelectron microscopy localized dsRNA in root tips systemically infected with barley stripe mosaic virus (BSMV). Anti-polyinosinic:polycytidylic acid (Anti-poly(I):poly(C)) serum was used as a primary stain followed by gold-labeled goat anti-rabbit IgG complexes. Only vesicles of vesiculated proplastids of early-infected cells but not proplastids of cells from noninoculated plants, contained dsRNA. This supports the notion of a role for proplastids in BSMV replication.
Lin
N S
NS
Department of Plant Pathology, University of Nebraska, Lincoln, Nebraska 68583, USA.
Langenberg
W G
WG