Influence of fluoroquinolones on phagocytosis and killing of Candida albicans by human polymorphonuclear neutrophils.
Candida albicans infections often occur during or shortly after antibacterial treatment. Phagocytosis by polymorphonuclear neutrophil granulocytes (PMN) is the most important primarily defence mechanism against C. albicans. Certain antibiotics such as some fluoroquinolones (FQ) are known to influence phagocyte functions. Thus, we investigated the influence of older and newer FQ on the phagocytosis and killing of C. albicans by human PMN paying special attention to CD11b expression of these cells as an indicator of the degree of their activation. In order to obtain comprehensive and comparable results we tested 13 FQ over a wide range of concentrations and in a time dependent manner in a standardized approach. When used at therapeutic concentrations, the FQ tested did not influence to a clinically significant degree the phagocytosis or the killing of C. albicans by human PMN and also not their activation. However, at high concentrations those FQ with cyclopropyl-moiety at position N1 showed increase in CD11b expression and diminished phagocytosis and oxidative burst.
Gruger
Thomas
T
Federal Institute for Drugs and Medical Devices (BfArM), Biosafety Laboratory, Bonn, Germany.
Morler
Caroline
C
Schnitzler
Norbert
N
Brandenburg
Kerstin
K
Nidermajer
Sabine
S
Horre
Regine
R
Zundorf
Josef
J
D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D039481:CD11b Antigen / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology*; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D016897:Respiratory Burst / Q000187:drug effects; D055815:Young Adult
D000900:Anti-Bacterial Agents; D039481:CD11b Antigen; D024841:Fluoroquinolones