title: Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids.
abstract: A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.
authors.affiliation: Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Japan.
authors.forename: Fumi
authors.initials: F
authors.lastname: Nagatsugi
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Nakahara
authors.forename: Kaori
authors.initials: K
authors.lastname: Inoue
authors.forename: Shigeki
authors.initials: S
authors.lastname: Sasaki
mesh_terms: D002846:Chromatography, Affinity; D008156:Luciferases / Q000138:chemical synthesis* / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D009841:Oligonucleotides; D011092:Polyethylene Glycols; D008156:Luciferases