Rapid quantitative detection of Vibrio parahaemolyticus in seafood by MPN-PCR.
This study aimed to adopt MPN-PCR (most probable number-polymerase chain reaction) for rapid detection of the quantity of Vibrio parahaemolyticus in seafood. V. parahaemolyticus in seafood could be quantitated by MPN statistics according to PCR products. The sensitivity of MPN-PCR was 100 times higher than that of direct PCR. Of 225 seafood samples from Qingdao, 165 were positive for the presence of V. parahaemolyticus, with an MPN value of >719 per gram, and about 41.5% of samples were positive for tdh gene-possessing cells. Eighty muscle tissues from the 225 seafood samples were investigated by direct PCR and MPN-PCR, but no V. parahaemolyticus was detected. The MPN-PCR test could be completed in less than 16 h from the time of sample preparation. It was rapid, sensitive, and reliable for comprehensive detection and quick quantitative determination of V. parahaemolyticus in seafood and it revealed the potential risk of illness associated with their consumption.
Luan
Xiaoyan
X
Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, 5 Yushan Road, Qingdao, China.
Chen
Jixiang
J
Liu
Yu
Y
Li
Yun
Y
Jia
Juntao
J
Liu
Rui
R
Zhang
Xiao-Hua
XH
D016133:Polymerase Chain Reaction / Q000379:methods; D017747:Seafood / Q000382:microbiology*; D014736:Vibrio parahaemolyticus / Q000302:isolation & purification / Q000378:metabolism*