Cucumber mosaic virus and peanut stunt virus differ in an initial event that occurs during the infection of cowpea leaf epidermal cells.
Using procedures described earlier for peanut stunt virus (PSV) (G. I. Mink, Virology 72, 291-298, 1976), hydroxylamine, ribonuclease (RNase), and tetrachloro-o-benzoquinone were used to determine 10-min inactivation profiles for cucumber mosaic virus (CMV) and CMV-RNA. These profiles indicate that the first event that is detectable by the use of inactivating agents in the infection of Cowpea primary leaves by either CMV or CMV-RNA differed significantly from that reported earlier for PSV and PSV-RNA. The results suggested that a fraction of the intact CMV applied to Cowpea leaves was somehow protected from the effects of inactivating agents immediately upon inoculation. This phenomenon was eliminated by neutralizing the strong negative surface charge of CMV with cytochrome c. During the first 10-min interval after inoculation CMV and CMV-RNA appear to be attached to some stationary cell component. The results indicate that Cowpea epidermal cells can distinguish almost instantly between CMV and PSV or between their respective RNAs.
Ehara
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Department of Plant Pathology, Irrigated Agriculture Research and Extension Center, Prosser, Washington 99350, USA.
Mink
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GI