title: Effects of multiple-bond ruptures on kinetic parameters extracted from force spectroscopy measurements: revisiting biotin-streptavidin interactions.
abstract: Force spectroscopy measurements of the rupture of the molecular bond between biotin and streptavidin often results in a wide distribution of rupture forces. We attribute the long tail of high rupture forces to the nearly simultaneous rupture of more than one molecular bond. To decrease the number of possible bonds, we employed hydrophilic polymeric tethers to attach biotin molecules to the atomic force microscope probe. It is shown that the measured distributions of rupture forces still contain high forces that cannot be described by the forced dissociation from a deep potential well. We employed a recently developed analytical model of simultaneous rupture of two bonds connected by polymer tethers with uneven length to fit the measured distributions. The resulting kinetic parameters agree with the energy landscape predicted by molecular dynamics simulations. It is demonstrated that when more than one molecular bond might rupture during the pulling measurements there is a noise-limited range of probe velocities where the kinetic parameters measured by force spectroscopy correspond to the true energy landscape. Outside this range of velocities, the kinetic parameters extracted by using the standard most probable force approach might be interpreted as artificial energy barriers that are not present in the actual energy landscape. Factors that affect the range of useful velocities are discussed.
authors.affiliation: Department of Chemistry, Duke University, Durham, North Carolina 27708, USA.
authors.forename: Senli
authors.initials: S
authors.lastname: Guo
authors.forename: Chad
authors.initials: C
authors.lastname: Ray
authors.forename: Andrea
authors.initials: A
authors.lastname: Kirkpatrick
authors.forename: Nimit
authors.initials: N
authors.lastname: Lad
authors.forename: Boris B
authors.initials: BB
authors.lastname: Akhremitchev
mesh_terms: D001696:Biomechanical Phenomena; D001710:Biotin / Q000378:metabolism*; D003198:Computer Simulation; D007700:Kinetics; D008958:Models, Molecular; D013057:Spectrum Analysis / Q000379:methods*; D019809:Streptavidin / Q000378:metabolism*
chemical_list: D001710:Biotin; D019809:Streptavidin