title: A new serotype 14 variant of the pneumococcal Spain9V-3 international clone detected in the central region of Argentina.
abstract: The penicillin-resistant Spain(9V)-3 clone of Streptococcus pneumoniae is widespread and presents different serotype variants originating from recombination of the capsular genes. In this work, the genetic relatedness of 29 invasive pneumococci isolated from the central region of Argentina (Cordoba, Buenos Aires, Santa Fe and La Pampa provinces) was assessed by multilocus sequence typing (MLST). All of the penicillin-non-susceptible isolates studied (21/29) belonged to a serotype 14 variant of the Spain(9V)-3 clone. This clone was predominant, suggesting that it was responsible for the penicillin resistance spread in this region. Interestingly, this serotype 14 variant (named Cordoba S14V) could be differentiated from the European one by its pbp1a gene, suggesting a different recombinational replacement of the capsular genes. The putative recombination sites were analysed, resulting in the proximal crossover point being clearly localized in the spr0309 gene, with the distal site restricted to the recU gene, confirming a different recombination event. Analysis of the dexB, cpsB, aliA and pbp1a genes from these strains showed a high similarity with the corresponding genes of the Spain(14)-5 clone, suggesting that the capsular genes were provided by this international clone. Analysis of the genetic polymorphisms of the pbp1a (nt 1473-1922) and spr0309 (nt 1-790) genes is proposed as an epidemiological tool to help recognize the Cordoba S14V of the Spain(9V)-3 clone. On the other hand, BOX-repeat-based PCR and MLST analyses of serotype 14 strains revealed a divergent epidemiology of the Cordoba S14V, suggesting a non-recent dissemination in the paediatric population. It is suggested that this molecular epidemiology work will be a reference for monitoring the evolution of S14Vs of Spain(9V)-3, the emergence of new clones and the impact of pneumococcal vaccination programmes in Argentina.
authors.affiliation: Departamento de Bioquimica Clinica-CIBICI (CONICET), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina.
authors.forename: Andrea G
authors.initials: AG
authors.lastname: Albarracín Orio
authors.affiliation: Hospital Pediatrico del Niño Jesus, Cordoba, Argentina.|Centro de Desarrollo de Proyectos Avanzados en Pediatria (CEDEPAP), Cordoba, Argentina.|Departamento de Bioquimica Clinica-CIBICI (CONICET), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina.
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Cortes
authors.affiliation: Centro de Desarrollo de Proyectos Avanzados en Pediatria (CEDEPAP), Cordoba, Argentina.
authors.forename: Miguel
authors.initials: M
authors.lastname: Tregnaghi
authors.affiliation: Departamento de Bioquimica Clinica-CIBICI (CONICET), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina.
authors.forename: German E
authors.initials: GE
authors.lastname: Piñas
authors.affiliation: Departamento de Bioquimica Clinica-CIBICI (CONICET), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina.
authors.lastname: Argentinean Network Pneumococcus Study Group
authors.affiliation: Departamento de Bioquimica Clinica-CIBICI (CONICET), Facultad de Ciencias Quimicas, Universidad Nacional de Cordoba, Cordoba, Argentina.
authors.forename: José R
authors.initials: JR
authors.lastname: Echenique
mesh_terms: D001118:Argentina; D001483:Base Sequence; D001769:Blood / Q000382:microbiology; D003001:Cloning, Molecular; D017931:DNA Primers; D003710:Demography; D014644:Genetic Variation; D006801:Humans; D008826:Microbial Sensitivity Tests; D008969:Molecular Sequence Data; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012703:Serotyping; D013296:Streptococcus pneumoniae / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D017931:DNA Primers