title: Leukemia inhibitory factor secretion is a predictor and indicator of early progenitor status in adult bone marrow stromal cells.
abstract: Bone marrow-derived stromal cells (BMSCs) are defined by their ability to self-renew and differentiate into at least three mesenchymal cell types (bone, adipose, and cartilage). The inability to isolate a reliably efficacious and homogeneous population of early progenitor cells has limited efforts to increase their therapeutic potential. In this study, we focused on identifying protein markers that may be employed to predict the efficacy of a cultured BMSC population. Markers of progenitor status were identified by comparing BMSCs at early and late passage, donor-matched skin fibroblasts, and commercially available dermal fibroblast cell lines. Differentiation potential was determined according to in vitro assays of osteogenesis, adipogenesis, and chondrogenesis. Early-passage BMSCs differentiated into all three lineages, whereas late-passage BMSCs and both fibroblast preparations did not. To identify novel markers of early progenitors, microarray transcript analysis between early-passage BMSCs and fibroblasts was performed. Messenger RNA encoding the cytokine leukemia inhibitory factor (LIF) was identified as differentially expressed. Enzyme-linked immunosorbent assay on conditioned media confirmed that LIF secretion was much higher from early progenitor BMSCs than donor-matched or commercial lines of fibroblasts and dropped with extensive expansion or induction of differentiation. In clonally expanded BMSCs, colonies that retained progenitor status expressed significantly higher levels of LIF than those that failed to differentiate. Our results indicate that LIF expression may represent a marker to quantify the differentiation potential of BMSCs and may be especially suited for the rapid, noninvasive quality control of clinical preparations.
authors.affiliation: Center for Gene Therapy, Tulane University Health Sciences Center, New Orleans, Louisiana 70112, USA.
authors.forename: Mandolin J
authors.initials: MJ
authors.lastname: Whitney
authors.forename: Andy
authors.initials: A
authors.lastname: Lee
authors.forename: Joni
authors.initials: J
authors.lastname: Ylostalo
authors.forename: Suzanne
authors.initials: S
authors.lastname: Zeitouni
authors.forename: Alan
authors.initials: A
authors.lastname: Tucker
authors.forename: Carl A
authors.initials: CA
authors.lastname: Gregory
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism; D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis / Q000378:metabolism; D001057:Apolipoproteins E / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002460:Cell Line; D002478:Cells, Cultured; D002648:Child; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D053605:Leukemia Inhibitory Factor / Q000032:analysis / Q000378:metabolism*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000201:enzymology / Q000378:metabolism*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D053244:Osteoprotegerin / Q000032:analysis; D012867:Skin / Q000166:cytology; D017154:Stromal Cells / Q000166:cytology*
chemical_list: D001057:Apolipoproteins E; D053605:Leukemia Inhibitory Factor; D053244:Osteoprotegerin; D000469:Alkaline Phosphatase