Effect of 2,4-dichlorophenoxyacetic acid on multiplication of tobacco mosaic virus in protoplasts from local-lesion and systemic-responding tobaccos.
Tobacco mosaic virus (TMV) multiplication was markedly enhanced in protoplasts from Samsun NN and Xanthi-nc, two local-lesion-responding cultivars, by 2,4-dichlorophenoxyacetic acid (2,4-D) added to the incubation medium after inoculation of the protoplasts. In Samsun, a cultivar which reacts systemically to TMV infection, 2,4-D reduced virus multiplication. These findings may explain the discrepancy observed between the low virus multiplication in intact tobaccos with the N gene and the high titers reached in protoplasts from these plants (in the presence of 2,4-D), which were similar to those found in protoplasts from a systemic cultivar.
Loebenstein
G
G
Virus Laboratory, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel.
Gera
A
A
Barnett
A
A
Shabtai
S
S
Cohen
J
J