Mapping early transcription products of Autographa californica nuclear polyhedrosis virus.
The regions of the AcMNPV genome represented as cytoplasmic transcripts early after infection of Spodoptera frugiperda cells prior to and encompassing the initiation of DNA replication were mapped. In vivo(32)P pulse-labeled cytoplasmic RNA from infected cells at various times postinfection was used to probe Southern blots of cloned EcoRI AcMNPV DNA fragments. At the earliest time point studied (0.5 - 2.5 hr p.i.) transcripts represented a large proportion of the genome although specific regions were predominantly transcribed (EcoRI A, C, F, J, O, Q, S, and T). After the onset of viral DNA synthesis predominant cytoplasmic transcripts represented more dispersed areas of the genome, particularly those regions which were only weakly transcribed earlier after infection. Genomic regions containing major late genes were not predominantly transcribed by 7.5 hr p.i.
Erlandson
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MA
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario K7L 3N6, Canada.
Carstens
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EB