title: D2: major subgenotype of hepatitis B virus in Russia and the Baltic region.
abstract: Complete or almost complete hepatitis B virus (HBV) genomes were sequenced for 13 genotype A and 42 genotype D strains from the former USSR. The strains were classifiable within subgenotypes A2, D1, D2 and D3. Comparison of the deduced gene products for the four ORFs of 89 genotype D strains revealed 27 subgenotype-specific residues, and a region spanning residues 58-128 in the spacer region of the P gene could be used to distinguish between D1 and D4. This enabled the allocation to subgenotype of strains with partially sequenced genomes. D2 was dominating, while D3 was found in low frequency in the whole region. D1 was most prevalent in the Middle Asian Republics. Mean inter-subgenotype divergences between D1 and D2, D1 and D3 and D2 and D3 were 2.7, 3.4 and 3.4 %, respectively. The intra-subgenotype divergence was 0.4, 1.1, 1.0 and 1.8 % for A2, D1, D2 and D3, respectively. All D1 and D3 strains encoded subtype ayw2, whereas most D2 strains encoded ayw3. Two D2 strains encoded ayw4. Strains with identical S genes were closely related at the level of complete genomes and formed geographically specific clades with low intraclade divergences, possibly indicating past iatrogenic spread. It is not clear whether the finding of four subgenotypes in the area corresponds to separate introductions of the virus or to previous population migrations into the area. An earlier introduction of D3 compared with D2 was supported by its higher intra-subgenotype divergence, while the lower divergence within D1 is probably due to a more recent emergence.
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden.|Department of Virology, Immunology and Vaccinology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden.|Department of Virology, National Institute for Health Development, Tallinn, Estonia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Tallo
authors.affiliation: Department of Virology, National Institute for Health Development, Tallinn, Estonia.
authors.forename: Valentina
authors.initials: V
authors.lastname: Tefanova
authors.affiliation: Department of Virology, National Institute for Health Development, Tallinn, Estonia.
authors.forename: Ljudmilla
authors.initials: L
authors.lastname: Priimägi
authors.affiliation: Department for Infectious Disease, Ida-Viru Central Hospital, Kohtla-Järve, Estonia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Schmidt
authors.affiliation: Department of Virology, National Institute for Health Development, Tallinn, Estonia.
authors.forename: Olga
authors.initials: O
authors.lastname: Katargina
authors.affiliation: MP Chumakov Institute of Poliomyelitis and Viral Encephalitis, Moscow, Russia.
authors.forename: Michail
authors.initials: M
authors.lastname: Michailov
authors.affiliation: St Petersburg Pasteur Institute, St Petersburg, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Mukomolov
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden.|Department of Virology, Immunology and Vaccinology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden.
authors.forename: Lars
authors.initials: L
authors.lastname: Magnius
authors.affiliation: Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden.|Department of Virology, Immunology and Vaccinology, Swedish Institute for Infectious Disease Control, Stockholm, Sweden.
authors.forename: Heléne
authors.initials: H
authors.lastname: Norder
mesh_terms: D001454:Baltic States / Q000453:epidemiology; D004279:DNA, Viral / Q000032:analysis / Q000302:isolation & purification; D016679:Genome, Viral; D005838:Genotype; D006509:Hepatitis B / Q000453:epidemiology / Q000821:virology; D006515:Hepatitis B virus / Q000145:classification* / Q000235:genetics*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D012426:Russia / Q000453:epidemiology; D017422:Sequence Analysis, DNA; D014764:Viral Proteins / Q000235:genetics
chemical_list: D004279:DNA, Viral; D014764:Viral Proteins