title: Proteomics of multigenic families from species underrepresented in databases: the case of loquat (Eriobotrya japonica Lindl.) polyphenol oxidases.
abstract: Here, we approach the problem of obtaining accurate and reliable information about the gene origin of a protein belonging to a multigenic family, polyphenol oxidase, from an underrepresented species, Eriobotrya japonica. De novo sequencing was a key approach to obtain broad sequence coverage. Alignment of peptides on their most similar homologous protein revealed divergent amino acid positions that lead to hypothesize the minimal number of genes encoding for the proteins analyzed.
authors.affiliation: Grupo de Proteomica y Genomica Funcional de Plantas, Departamento de Agroquimica y Bioquimica, Facultad de Ciencias, Universidad de Alicante, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Sellés-Marchart
authors.forename: Ignacio
authors.initials: I
authors.lastname: Luque
authors.forename: Juan
authors.initials: J
authors.lastname: Casado-Vela
authors.forename: Maria José
authors.initials: MJ
authors.lastname: Martínez-Esteso
authors.forename: Roque
authors.initials: R
authors.lastname: Bru-Martínez
mesh_terms: D000595:Amino Acid Sequence; D000917:Antibody Formation; D015153:Blotting, Western; D004156:Catechol Oxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D031983:Eriobotrya / Q000201:enzymology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012995:Solubility
chemical_list: D004156:Catechol Oxidase