title: Comparison of polymerase chain reaction of polymorphonuclear leukocytes and plasma identifies patients who control cytomegalovirus infection after hematopoietic cell transplantation.
abstract: By use of an automated polymerase chain reaction test of plasma and a qualitative polymerase chain reaction assay on polymorphonuclear leukocytes, we identified a subgroup of hematopoietic cell transplant recipients who were able to control cytomegalovirus infection early after hematopoietic cell transplantation without antiviral therapy. Thirty-one percent of patients had cytomegalovirus DNA detected by qualitative polymerase chain reaction assay but had no cytomegalovirus DNA detected by the automated test; this group maintained a lower peak cytomegalovirus load, compared with the group of patients who had cytomegalovirus DNA detected by both tests (P = .03), suggesting a greater degree of functional immune reconstitution.
authors.affiliation: Department of Microbiology, Stanford University School of Medicine, Stanford, California, USA.
authors.forename: Marcy L
authors.initials: ML
authors.lastname: Vana
authors.forename: Danuse
authors.initials: D
authors.lastname: Formankova
authors.forename: Steven
authors.initials: S
authors.lastname: Cha
authors.forename: Anjali
authors.initials: A
authors.lastname: Sharma
authors.forename: Luciano
authors.initials: L
authors.lastname: Potena
authors.forename: Janice M Y
authors.initials: JM
authors.lastname: Brown
authors.forename: Edward S
authors.initials: ES
authors.lastname: Mocarski
mesh_terms: D000328:Adult; D003587:Cytomegalovirus / Q000235:genetics / Q000302:isolation & purification*; D003586:Cytomegalovirus Infections / Q000276:immunology* / Q000821:virology; D004279:DNA, Viral / Q000032:analysis / Q000097:blood*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000009:adverse effects*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000821:virology*; D010949:Plasma / Q000821:virology*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004279:DNA, Viral