title: Improvement of the total turnover number and space-time yield for chloroperoxidase catalyzed oxidation.
abstract: Chloroperoxidase from Caldariomyces fumago was applied for the oxidation of indole to oxindole using hydrogen peroxide as the oxidant in aqueous t-butyl alcohol medium. Different ways of adding the oxidant, various reactor types, and the use of a hydrogen peroxide-stat were compared, resulting in a 20-fold increase of the total turnover number (ttn) and space-time yield (sty). The highest ttn of >860,000 was obtained in a fed-batch reactor, whereas the highest sty of 120 g/(L . d) was reached in a continuously operated enzyme membrane reactor. The results were compared to other enzyme systems already established for the synthesis of amino acids and carbohydrates. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 283-288, 1997.
authors.affiliation: Forschungszentrum Jülich GmbH, Institut für Biotechnologie, D-52425 Jülich, Germany.
authors.forename: K
authors.initials: K
authors.lastname: Seelbach
authors.forename: M P
authors.initials: MP
authors.lastname: van Deurzen
authors.forename: F
authors.initials: F
authors.lastname: van Rantwijk
authors.forename: R A
authors.initials: RA
authors.lastname: Sheldon
authors.forename: U
authors.initials: U
authors.lastname: Kragl