title: Array and distribution of actin filaments in guard cells contribute to the determination of stomatal aperture.
abstract: Actin filaments in guard cells and their dynamics function in regulating stomatal movement. In this study, the array and distribution of actin filaments in guard cells during stomatal movement were studied with two vital labeling, microinjection of alexa-phalloidin in Vicia faba and expression of GFP-mTn in tobacco. We found that the random array of actin filaments in the most of the closed stomata changed to a ring-like array after stomatal open. And actin filaments, which were throughout the cytoplasm of guard cells of closed stomata (even distribution), were mainly found in the cortical cytoplasm in the case of open stomata (cortical distribution). These results revealed that the random array and even distribution of actin filaments in guard cells may be required for keeping the closed stomata; similarly, the ring-like array and cortical distribution of actin filaments function in sustaining open stomata. Furthermore, we found that actin depolymerization, the trait of moving stomata, facilitates the transformation of actin array and distribution with stomatal movement. So, the depolymerization of actin filaments was favorable for the changes of actin array and distribution in guard cells and thus facilitated stomatal movement.
authors.affiliation: State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University 100094, Beijing, People's Republic of China.
authors.forename: Xin-Qi
authors.initials: XQ
authors.lastname: Gao
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.forename: Peng-Cheng
authors.initials: PC
authors.lastname: Wei
authors.forename: Fei
authors.initials: F
authors.lastname: Ren
authors.forename: Jia
authors.initials: J
authors.lastname: Chen
authors.forename: Xue-Chen
authors.initials: XC
authors.lastname: Wang
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008845:Microinjections; D010590:Phalloidine / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism; D054046:Plant Stomata / Q000166:cytology* / Q000502:physiology*; D011108:Polymers / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D014026:Nicotiana / Q000166:cytology; D014170:Transformation, Genetic; D031307:Vicia faba / Q000166:cytology
chemical_list: D010940:Plant Proteins; D011108:Polymers; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins; D010590:Phalloidine