title: A sulfated glucuronofucan containing both fucofuranose and fucopyranose residues from the brown alga Chordaria flagelliformis.
abstract: A fucoidan fraction composed of l-fucose, sulfate, and d-glucuronic acid in a molar proportion of about 1:1:0.25 and small amount of acetyl groups was isolated from the brown alga Chordaria flagelliformis. Several modified polysaccharides were prepared from the native fucoidan using solvolytic desulfation, carboxyl reduction, and partial acid hydrolysis. Polysaccharide structures were elucidated by methylation analysis and 1D and 2D NMR spectroscopy. The fucoidan was shown to contain a backbone of 3-linked alpha-l-fucopyranose residues, about one-third of which are glycosylated at C-2 by alpha-d-glucopyranosyluronic acid residues. About half of the latter residues are glycosylated at C-4 by single alpha-L-fucofuranose residues or by disaccharides alpha-L-Fucf-(1-->2)-alpha-L-Fucf-(1-->. Fucofuranose residues are mono- and disulfated at different positions, whereas some additional sulfate groups occupy C-2 and C-4 of the backbone, the latter position being also partially acetylated.
authors.affiliation: Russian Academy of Sciences, Leninskii Prosp., 47, 119991 Moscow, Russian Federation.
authors.forename: Maria I
authors.initials: MI
authors.lastname: Bilan
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Vinogradova
authors.forename: Evgenia A
authors.initials: EA
authors.lastname: Tsvetkova
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Grachev
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Shashkov
authors.forename: Nikolay E
authors.initials: NE
authors.lastname: Nifantiev
authors.forename: Anatolii I
authors.initials: AI
authors.lastname: Usov
mesh_terms: D018533:Biomass; D005643:Fucose / Q000737:chemistry; D005663:Furans / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006031:Glycosylation; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy; D008745:Methylation; D008968:Molecular Conformation; D000459:Phaeophyceae / Q000378:metabolism*; D011134:Polysaccharides / Q000737:chemistry; D011714:Pyrans / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry; D013463:Sulfuric Acid Esters
chemical_list: D005663:Furans; D011134:Polysaccharides; D011714:Pyrans; D013431:Sulfates; D013463:Sulfuric Acid Esters; D005643:Fucose; D020723:Glucuronic Acid; C007789:fucoidan