title: A study of TMV ts mutant Ni2519. I. Complementation experiments.
abstract: Two distinct virus-specific functions, i.e., virus assembly and spreading of infection from cell to cell (transport function), are temperature-sensitive (ts) in TMV mutant Ni2519. Assembly of Ni2519 cannot be complemented by the temperature-resistant TMV strains used: A14 (a wild type strain from which Ni2519 was derived) and dolichos enation mosaic virus (DEMV, or cowpea strain of TMV), a thermophilic strain. On the other hand, Ni2519 can serve as a donor of the coat protein to complement is strain Ni118, which has a mutation in the coat protein gene. The genomic RNA can be produced by Ni2519 at a nonpermissive temperature; functionally active Ni2519 coat protein (capable of coating Ni118 RNA upon mixed infection) is produced at a nonpermissive temperature as well. The is phenotype of Ni2519 upon virus assembly probably results not from the ts behavior of any virus-coded protein(s) but is due to the ts properties of the genomic RNA molecule itself, so the possibility of the complementation of assembly of Ni2519 is ruled out. Thus, Ni2519 appears to represent a novel class of virus mutants with is virion RNA. The second is function of Ni2519 (transport of infection) can be complemented by a helper virus. The experimental system used for complementation of the transport function allowed Ni2519 to spread from cell to cell at a nonpermissive temperature. Obviously, Ni2519 infection spreads under these conditions in a form different from that in the mature virions, since its assembly cannot be complemented by the helper virus. Some aspects of the transport function are discussed.
authors.affiliation: Department of Virology and A. N. Belazersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, Moscow 117234, USSR.
authors.forename: M E
authors.initials: ME
authors.lastname: Taliansky
authors.forename: T I
authors.initials: TI
authors.lastname: Atabekova
authors.forename: I B
authors.initials: IB
authors.lastname: Kaplan
authors.forename: S Y
authors.initials: SY
authors.lastname: Morozov
authors.forename: S I
authors.initials: SI
authors.lastname: Malyshenko
authors.forename: J G
authors.initials: JG
authors.lastname: Atabekov