title: Development of a pen-site test kit for the rapid diagnosis of H7 highly pathogenic avian influenza.
abstract: As well as H5 highly pathogenic avian influenza viruses (HPAIV), H7 HPAIV strains have caused serious damages in poultry industries worldwide. Cases of bird-to-human transmission of H7 HPAIV have also been reported [11]. On the outbreak of avian influenza, rapid diagnosis is critical not only for the control of HPAI but also for human health. In the present study, a rapid diagnosis kit based on immunochromatography for the detection of H7 hemagglutinin (HA) antigen of influenza A virus was developed using 2 monoclonal antibodies that recognize different epitopes on the H7 HAs. The kit detected each of the tested 15 H7 influenza virus strains and did not react with influenza A viruses of the other subtypes than H7 or other avian viral and bacterial pathogens. The kit detected H7 HA antigen in the swabs and tissue homogenates of the chickens experimentally infected with HPAIV strain A/chicken/Netherlands/2586/03 (H7N7). The results indicate that the present kit is specific and sensitive enough for the diagnosis of HPAI caused by H7 viruses, thus, recommended for the field application as a pen-site test kit.
authors.affiliation: Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
authors.forename: Rashid
authors.initials: R
authors.lastname: Manzoor
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Sakoda
authors.forename: Saori
authors.initials: S
authors.lastname: Sakabe
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Mochizuki
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Namba
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Tsuda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kida
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D002645:Chickens; D002845:Chromatography / Q000379:methods; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000276:immunology / Q000302:isolation & purification*; D053126:Influenza A Virus, H7N7 Subtype / Q000145:classification / Q000472:pathogenicity*; D005585:Influenza in Birds / Q000175:diagnosis* / Q000821:virology; D011933:Reagent Kits, Diagnostic / Q000662:veterinary* / Q000821:virology
chemical_list: D000911:Antibodies, Monoclonal; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011933:Reagent Kits, Diagnostic