Expression of cholera toxin B subunit in transgenic rice endosperm.
The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific promoter containing an intron of the rice act1. The recombinant vector was transformed into rice plants using a biolistic-mediated transformation method. Stable integration of the synthetic CTB gene into the chromosomal DNA was confirmed by PCR amplification analysis. A high level of CTB (2.1% of total soluble protein) was expressed in the endosperm tissue of the transgenic rice plants. The synthetic CTB produced only in the rice endosperm demonstrated strong affinity for G(M1)-ganglioside, thereby suggesting that the CTB subunits formed an active pentamer. The successful expression of CTB genes in transgenic plants makes it a powerful tool for the development of a plant-derived edible vaccine.
Oszvald
Maria
M
Department of Biochemistry and Food Technology, Budapest University of Technology and Economics, Budapest, Hungary.
Kang
Tae-Jin
TJ
Tomoskozi
Sandor
S
Jenes
Barnabas
B
Kim
Tae-Geum
TG
Cha
Youn-Soo
YS
Tamas
Laszlo
L
Yang
Moon-Sik
MS
D015152:Blotting, Northern; D015153:Blotting, Western; D002772:Cholera Toxin / Q000235:genetics*; D018744:DNA, Plant / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D012275:Oryza / Q000235:genetics*; D030821:Plants, Genetically Modified; D011956:Receptors, Cell Surface / Q000378:metabolism; D012639:Seeds / Q000235:genetics*
D018744:DNA, Plant; D011956:Receptors, Cell Surface; C034993:ganglioside receptor; D002772:Cholera Toxin