Site-specific dense immobilization of antibody fragments on polymer brushes supported by silicone nanofilaments.
For site-specific dense immobilization of antibodies on a solid support, we prepared phosphorylcholine copolymer brushes on silicone nanofilaments. The nanofilaments were prepared on a silicon wafer by treatment with trichloromethylsilane (MeSiCl 3). To generate Si-OH groups on the nanofilaments, O 2 plasma was irradiated on the surface. Initiators for atom transfer radical polymerization (ATRP) were then coupled on the filaments. Phosphorylcholine copolymer brushes were prepared by a "grafting from" process, and pyridyl disulfide groups were introduced into the polymer chains. F(ab') fragments were then specifically immobilized onto these surfaces via a thiol-disulfide interchange reaction. The amount of antibodies immobilized on the nanofilament-supported copolymer brushes was approximately 65 times greater than that on smooth wafer-supported copolymer brushes.
Iwasaki
Yasuhiko
Y
Departments of Chemistry and Materials Engineering, Faculty of Chemistry, Materials and Bioengineering, Kansai University, 3-3-35 Yamate-cho, Suita-shi, Osaka, Japan. yasu.bmt@ipcku.kansai-u.ac.jp
Omichi
Yuki
Y
Iwata
Ryoko
R
D000906:Antibodies / Q000737:chemistry* / Q000276:immunology; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D011108:Polymers / Q000737:chemistry*; D012828:Silicones / Q000737:chemistry*
D000906:Antibodies; D011108:Polymers; D012828:Silicones