title: Isolation and characterization of a double-stranded DNA mycovirus infecting the aquatic fungus, Rhizidiomyces.
abstract: Isometric particles 60 nm in diameter were isolated from the culture filtrate of the aquatic fungus, Rhizidiomyces, via pressure dialysis. The virus particles had a sedimentation coefficient of 625 S and a buoyant density of CsCI of 1.314 g/ml. The nucleic acid was dsDNA present as a single molecule (MW = 16.8 x 10(6)). Gel electrophoresis revealed 14+ protein bands ranging from MW of 84,500 to 26,000, with the largest one being dominant. This mycovirus appears to be distinct from all previously characterized viruses.
authors.affiliation: Department of Plant Pathology, University of Georgia, Athens, Georgia 30602, USA.
authors.forename: V H
authors.initials: VH
authors.lastname: Dawe
authors.forename: C W
authors.initials: CW
authors.lastname: Kuhn