title: Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.
abstract: The Red recombinase system of bacteriophage Lambda has been used to inactivate chromosomal genes in bacteria using PCR products. In this study, we describe the replacement of the ampicillin resistance marker of helper plasmids pKD46 and pCP20 by a gentamicin resistance gene to disrupt chromosomal genes and then to eliminate FRT flanked resistance gene in multiple antibiotic-resistant Salmonella enterica strains.
authors.affiliation: INRA, UR1282, Infectiologie Animale et Santé Publique, Nouzilly, 37380, France. Benoit.Doublet@tours.inra.fr
authors.forename: Benoît
authors.initials: B
authors.lastname: Doublet
authors.forename: Gregory
authors.initials: G
authors.lastname: Douard
authors.forename: Hayette
authors.initials: H
authors.lastname: Targant
authors.forename: Danièle
authors.initials: D
authors.lastname: Meunier
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Madec
authors.forename: Axel
authors.initials: A
authors.lastname: Cloeckaert
mesh_terms: D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D010582:Bacteriophage lambda / Q000201:enzymology* / Q000235:genetics; D002876:Chromosomes, Bacterial / Q000235:genetics; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D017353:Gene Deletion; D044404:Genomic Islands / Q000235:genetics; D005839:Gentamicins / Q000494:pharmacology; D010957:Plasmids / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D045522:Recombinases / Q000235:genetics* / Q000378:metabolism; D011995:Recombination, Genetic; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000235:genetics / Q000821:virology
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D045522:Recombinases; D000667:Ampicillin; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase