[Phylogeny analysis and identification of two bacterial strains sourcing from human intestine and having resistance to acid and bile].
OBJECTIVE
To screen bacteria for the engineering bacteria expressing and secreting high activity beta-galactosidase, and two bacterial strains called as R92-2 and R111 with acid and bile resistances would be isolated from health human intestine to strain identification and phylogenetic analysis.


METHODS
These two strains were first been identified with phenotype characteristic analysis. Then the 16S rDNAs of these two bacterial strains were amplified and sequenced with the primers designed by the conserve sequences. DNA sequences were blasted against GenBank, and the phylogenetic trees were constructed.


RESULTS
Phenotype characteristic analysis showed that both of the bacterial strains belonged to lactic acid bacteria. Results of Blastn showed that 16S rDNAs of R92-2 and Weissella cibaria had 100% homology; 16S rDNAs of R111 and Enterococcus faecium had 100% homology too.


CONCLUSIONS
R92-2 belongs to Weissella cibaria strain; R111 belongs to Enterococcus faecium strain. These two stains may be used to construct the engineering bacteria expressing and secreting high activity beta-galactosidase.
Wang
Chuan
C
Department of Medical Technology, West China School of Public Health, Sichuan University, Chengdu 610041, China.
Zhang
Chao-Wu
CW
Chen
Hui-Chao
HC
Yu
Qian
Q
Pei
Xiao-Fang
XF
Liu
Heng-Chuan
HC
D001483:Base Sequence; D001647:Bile Acids and Salts / Q000032:analysis / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial; D016984:Enterococcus faecium / Q000145:classification / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
D001647:Bile Acids and Salts; D012336:RNA, Ribosomal, 16S