title: Large-scale, high-resolution data acquisition system for extracellular recording of electrophysiological activity.
abstract: A platform for high spatial and temporal resolution electrophysiological recordings of in vitro electrogenic cell cultures handling 4096 electrodes at a full frame rate of 8 kHz is presented and validated by means of cardiomyocyte cultures. Based on an active pixel sensor device implementing an array of metallic electrodes, the system provides acquisitions at spatial resolutions of 42 microm on an active area of 2.67 mm x 2.67 mm, and in the zooming mode, temporal resolutions down to 8 micros on 64 randomly selected electrodes. The low-noise performances of the integrated amplifier (11 microV (rms)) combined with a hardware implementation inspired by image/video processing concepts enable high-resolution acquisitions with real-time preprocessing capabilities adapted to the handling of the large amount of acquired data.
authors.affiliation: Electronics and Signal Processing Laboratory, Institute of Microtechnology, University of Neuchâtel, CH-2000 Neuchâtel, Switzerland. kilian.imfeld@unine.ch
authors.forename: Kilian
authors.initials: K
authors.lastname: Imfeld
authors.forename: Simon
authors.initials: S
authors.lastname: Neukom
authors.forename: Alessandro
authors.initials: A
authors.lastname: Maccione
authors.forename: Yannick
authors.initials: Y
authors.lastname: Bornat
authors.forename: Sergio
authors.initials: S
authors.lastname: Martinoia
authors.forename: Pierre-André
authors.initials: PA
authors.lastname: Farine
authors.forename: Milena
authors.initials: M
authors.lastname: Koudelka-Hep
authors.forename: Luca
authors.initials: L
authors.lastname: Berdondini
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002478:Cells, Cultured; D004594:Electrophysiology / Q000295:instrumentation*; D008564:Membrane Potentials / Q000502:physiology*; D008839:Microelectrodes; D032383:Myocytes, Cardiac / Q000166:cytology* / Q000502:physiology*; D051381:Rats; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*; D014741:Video Recording / Q000295:instrumentation*