Identification of unreported putative new bovine papillomavirus types in Brazilian cattle herds.
The amplification by degenerate primers FAP59/FAP64 and sequencing allowed the detection of 15 putative new BPV types in cutaneous warts as well as in healthy skin. Four of these isolates were recently recognized as new BPV types (BPV-7, -8, -9, and -10) after determination of their complete genome sequences. In Brazil, investigations involving the definition of BPV types present in skin warts are still rare. The aim of the current study was to identify the BPV types associated with cutaneous papillomatosis observed in Brazilian cattle herds. Twenty-two cutaneous papilloma specimens were submitted to PCR assay employing the FAP primer pair. All PCR products with approximately 480 bp were submitted to direct sequencing. Cloning was performed for the amplicons which prior analysis revealed as putative new BPV types. From 16 cutaneous lesions, BPV-1, -2, and -6 were identified in two, six, and eight papilloma specimens, respectively. In addition, four putative new BPV types were identified in other six skin warts, and then designated as BPV/BR-UEL2 to -5. The detection of the BPV-1, -2, and -6 types in skin wart specimens supports the existence of these BPV types throughout the Brazilian cattle herd. In addition, the identification of four putative new BPV types is the first report of the presence of different BPV types in the American continent.
Claus
Marlise Pompeo
MP
Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, P.O. Box 6001, 86051-990 Londrina, Parana, Brazil.
Lunardi
Michele
M
Alfieri
Alice Fernandes
AF
Ferracin
Lara Munique
LM
Fungaro
Maria Helena Pelegrinelli
MH
Alfieri
Amauri Alcindo
AA
D000818:Animals; D001938:Brazil / Q000453:epidemiology; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000821:virology*; D004279:DNA, Viral / Q000235:genetics; D052687:Deltapapillomavirus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal; D030361:Papillomavirus Infections / Q000453:epidemiology / Q000662:veterinary* / Q000821:virology
D004279:DNA, Viral; D004365:Drugs, Chinese Herbal; C476037:PHY 906