[Evaluation of insulin-like growth factor-1 influence on PECAM-1 and ICAM-1 adhesion molecules expression on human umbilical endothelial cells surface].
Activation, proliferation and regeneration of endothelium form developmental background of many pathological states. In those processes adhesion molecules and growth factors are crucial. Insulin-like growth factor-1 (IGF-1) is important regulator of vascular function, expressing pleiotropic actions. Interactions between endothelial cells and growth factors are complex and environment dependent. THE AIM OF THE STUDY was to assess IGF-1 influence on PECAM-1 and ICAM-1 adhesion molecules expression on human umbilical endothelial cells (HUVEC).


METHODS
Material for study was obtained from fresh umbilical cord. Cell lines were incubated with IGF-1 concentrations of 50 ng/ml and 200 ng/ml. Ratio of PECAM-1 and ICAM-1 expression revealing cells was obtained using the flow cytometry method. In statistic analysis ANOVA test and Duncan's new multiple range test were used.


RESULTS
It was demonstrated, that IGF-1 concentrations of 50 ng/ml and 200 ng/ml enhance PECAM-1 and ICAM-1 endothelial cells expression (respectively to 57.2+/-4.8%, 76.1+/-1.5% and 91.7+/-5.0%, 86.8+/-4.7%) compared to control group (respectively: 20.9+/-0.5% and 26.6+/-0.5%).


CONCLUSIONS
Insulin-like growth factor-1 enhances PECAM-1 and ICAM-1 adhesion molecules expression on HUVEC cells, activating the endothelium.
Szczepański
Marek
M
Akademia Medyczna w Białymstoku, Klinika Neonatologii i Intensywnej Terapii Noworodka.
Wnuczko
Krzysztof
K
D002460:Cell Line; D042783:Endothelial Cells / Q000378:metabolism*; D005434:Flow Cytometry; D015870:Gene Expression; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000494:pharmacology*; D018799:Intercellular Adhesion Molecule-1 / Q000096:biosynthesis*; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000096:biosynthesis*; D014471:Umbilical Veins / Q000378:metabolism
D036341:Intercellular Signaling Peptides and Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1; D007334:Insulin-Like Growth Factor I