Southern bean mosaic virus-specific proteins are synthesized in an in vitro system supplemented with intact, treated virions.
RNA encapsidated in icosahedral particles of southern bean mosaic virus (SBMV) can act as a template for protein synthesis in an mRNA-dependent rabbit reticulocyte cell-free translation system, following dialysis of virions against mildly alkaline buffers. Exposure of the SBMV RNA template occurs only after addition of virus particles to the translation system and appears not to involve complete disruption of the protective SBMV capsid.
Brisco
M J
MJ
Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, United Kingdom.
Hull
R
R
Wilson
T M
TM