title: Quantification of human chemokine production in TLR-stimulated and antigen-specific recall responses.
abstract: Chemokines are primarily low molecular mass proteins that are produced and usually released by a wide variety of cell types. Differential chemokine responses can be excellent early markers of immune dysfunction, allowing clinical intervention prior to expression of full blown undesirable effector responses. Thus, assessment of the nature and intensity of Ag-dependent chemokine production provides a valuable tool for probing human immune regulation.Here, we provide detailed instructions on approaches we have developed to assess the nature and intensity of recall responses to a wide variety of exogenous and endogenous antigens capable of consistently stimulating chemokine responses by PBMC from adult and pediatric populations. This chapter is divided into two sections. The first is focused on culture techniques for eliciting antigen-driven chemokine responses for a panel of chemokines that are relevant to immune function. The second section details assay systems for their quantitative analysis.
authors.affiliation: Department of Immunology, University of Manitoba, Winnipeg, Canada.
authors.forename: Monique
authors.initials: M
authors.lastname: Stinson
authors.forename: Renee
authors.initials: R
authors.lastname: Douville
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Lissitsyn
authors.forename: Melanie
authors.initials: M
authors.lastname: Blanchard
authors.forename: William
authors.initials: W
authors.lastname: Stefura
authors.forename: Estelle
authors.initials: E
authors.lastname: Simons
authors.forename: Allan
authors.initials: A
authors.lastname: Becker
authors.forename: Peter
authors.initials: P
authors.lastname: Nickerson
authors.forename: Kevin
authors.initials: K
authors.lastname: Coombs
authors.forename: Kent
authors.initials: K
authors.lastname: HayGlass
mesh_terms: D000941:Antigens / Q000276:immunology*; D018929:Cell Culture Techniques; D002478:Cells, Cultured; D019742:Chemokines, CC / Q000737:chemistry* / Q000276:immunology; D019743:Chemokines, CXC / Q000737:chemistry* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000737:chemistry / Q000166:cytology / Q000276:immunology*; D051193:Toll-Like Receptors / Q000276:immunology*
chemical_list: D000941:Antigens; D019742:Chemokines, CC; D019743:Chemokines, CXC; D051193:Toll-Like Receptors