title: A comparison of rapid-scanning X-ray fluorescence mapping and magnetic resonance imaging to localize brain iron distribution.
abstract: The clinical diagnosis of many neurodegenerative disorders relies primarily or exclusively on observed behaviors rather than measurable physical tests. One of the hallmarks of Alzheimer disease (AD) is the presence of amyloid-containing plaques associated with deposits of iron, copper and/or zinc. Work in other laboratories has shown that iron-rich plaques can be seen in the mouse brain in vivo with magnetic resonance imaging (MRI) using a high-field strength magnet but this iron cannot be visualized in humans using clinical magnets. To improve the interpretation of MRI, we correlated iron accumulation visualized by X-ray fluorescence spectroscopy, an element-specific technique with T1, T2, and susceptibility weighted MR (SWI) in a mouse model of AD. We show that SWI best shows areas of increased iron accumulation when compared to standard sequences.
authors.affiliation: Department of Anatomy and Cell Biology, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK, Canada.
authors.forename: Richard P E
authors.initials: RP
authors.lastname: McCrea
authors.forename: Sheri L
authors.initials: SL
authors.lastname: Harder
authors.forename: Melanie
authors.initials: M
authors.lastname: Martin
authors.forename: Richard
authors.initials: R
authors.lastname: Buist
authors.forename: Helen
authors.initials: H
authors.lastname: Nichol
mesh_terms: D000465:Algorithms; D000544:Alzheimer Disease / Q000000981:diagnostic imaging* / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging* / Q000378:metabolism* / Q000473:pathology; D021621:Imaging, Three-Dimensional / Q000379:methods; D007501:Iron / Q000032:analysis*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008822:Mice, Transgenic; D011856:Radiographic Image Enhancement / Q000379:methods; D011857:Radiographic Image Interpretation, Computer-Assisted / Q000379:methods; D012031:Refractometry / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013052:Spectrometry, X-Ray Emission / Q000379:methods*; D014018:Tissue Distribution; D014056:Tomography, X-Ray / Q000379:methods*
chemical_list: D007501:Iron