Plasma interleukin-1beta, -6, -8 and tumor necrosis factor-alpha as highly informative markers of pelvic inflammatory disease.
BACKGROUND
The role of proinflammatory cytokines in pelvic inflammatory disease (PID) is unclear. We therefore determined whether plasma proinflammatory cytokines, interleukin-1beta (IL-1beta), IL-6, IL-8 and tumor necrosis factor-alpha (TNF-alpha) were useful plasma markers in PID patients.


METHODS
Multiplex bead array analysis was used to measure the plasma levels of proinflammatory cytokines in 50 healthy controls as well as in 41 PID patients before and after routine protocol treatments.


RESULTS
IL-1beta, IL-6, IL-8 and TNF-alpha were significantly elevated in PID patients before antibiotic treatment than after treatment. However, IL-8 was not significantly different between healthy controls and PID patients. The relative increase in ratio of IL-6 was significantly correlated with white blood cell count (r=0.448, p=0.003), neutrophil count (r=0.472, p=0.002) and C-reactive protein level (r=0.412, p=0.008).


CONCLUSIONS
IL-1beta, IL-6, IL-8 and TNF-alpha may play an important role in the pathogenesis of PID. These biomarkers, particularly IL-6, could be useful adjuncts for the clinical diagnosis of PID.
Lee
Shun-An
SA
Institute of Medicine, Chung Shan Medical University, Lee's General Hospital, Ta-Chia, Taichung, Taiwan.
Tsai
Hsiu-Ting
HT
Ou
Hsiu-Chung
HC
Han
Chih-Ping
CP
Tee
Yi-Torng
YT
Chen
Yi-Chen
YC
Wu
Ming-Tsang
MT
Chou
Ming-Chin
MC
Wang
Po-Hui
PH
Yang
Shun-Fa
SF
D015415:Biomarkers / Q000097:blood; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D000292:Pelvic Inflammatory Disease / Q000097:blood / Q000175:diagnosis / Q000276:immunology*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood*
D015415:Biomarkers; D053583:Interleukin-1beta; D015850:Interleukin-6; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha