Injectable iron-modified apatitic bone cement intended for kyphoplasty: cytocompatibility study.
In this study, the cytocompatibility of human ephitelial (HEp-2) cells cultured on new injectable iron-modified calcium phosphate cements (IM-CPCs) has been investigated in terms of cell adhesion, cell proliferation, and morphology. Quantitative MTT-assay and scanning electron microscopy (SEM) showed that cell adhesion and viability were not affected with culturing time by iron concentration in a dose-dependent manner. SEM-cell morphology showed that HEp-2 cells, seeded on IM-CPCs, were able to adhere, spread, and attain normal morphology. These results showed that the new injectable IM-CPCs have cytocompatible features of interest to the intended kyphophasty application, for the treatment of osteoporotic vertebral compression fractures.
Vlad
M D
MD
Division of Biomaterials & Bioengineering, Interdepartment Research Group for the Applied Scientific Collaboration (IRGASC), Technical University of Catalonia (UPC), Avda. Diagonal 647, 08028, Barcelona, Spain.
del Valle
L J
LJ
Poeata
I
I
Barracó
M
M
López
J
J
Torres
R
R
Fernández
E
E
D001031:Apatites / Q000737:chemistry; D001672:Biocompatible Materials / Q000008:administration & dosage*; D001843:Bone Cements; D002130:Calcium Phosphates / Q000008:administration & dosage; D002277:Carcinoma / Q000473:pathology; D002448:Cell Adhesion; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D004305:Dose-Response Relationship, Drug; D004847:Epithelial Cells / Q000648:ultrastructure; D005562:Formazans / Q000032:analysis; D006801:Humans; D007267:Injections; D007501:Iron / Q000737:chemistry / Q000494:pharmacology; D007822:Laryngeal Neoplasms / Q000473:pathology; D013379:Substrate Specificity; D013778:Tetrazolium Salts / Q000032:analysis; D013997:Time Factors; D054854:Vertebroplasty / Q000379:methods*
D001031:Apatites; D001672:Biocompatible Materials; D001843:Bone Cements; D002130:Calcium Phosphates; D005562:Formazans; D013778:Tetrazolium Salts; C079782:MTT formazan; C020243:calcium phosphate; D007501:Iron