title: The adsorptive immobilization of phospholipids D mediated by calcium ions.
abstract: Immobilization of phospholipase D from cabbage was studied with the aim of stabilizing the enzyme for its use in synthesis of phospholipids. It was shown that phospholipase D can be immobilized by adsorption to polymeric carriers containing long chain anchor groups such as octadecyl, octyl, or other alkyl residues. Starting from the crude enzyme, phospholipase D activity is preferentially bound (up to 100%) in competition with contaminating proteins. A prerequisite of high binding rates is the presence of calcium ions, which play a mediating role in the adsorption process. The maximum activity of the carrier-enzyme complexes depends upon the calcium concentration in the immobilization process and the carrier material (> or = 10 mM CaCl(2) with octadecyl-Si40, > or = 40 mM CaCl(2) with octyl-sepharose and butyl-fractogel). Immobilization of phospholipase D to octyl-sepharose was shown to result in a distinctly increased storage stability and an enlarged pH-optimum range for the catalytic activity. Operational stability of different phospholipase D-carrier complexes was compared.
authors.affiliation: Martin-Luther University, Department of Biochemistry/Biotechnology, Weinbergweg, D-O-4050 Halle, Germany.
authors.forename: R
authors.initials: R
authors.lastname: Lambrecht
authors.forename: R
authors.initials: R
authors.lastname: Ulbrich-Hofmann