Molecular characterization and SNPs analysis of the porcine Deleted in AZoospermia Like (pDAZL) gene.
The Deleted in AZoospermia Like (DAZL) gene is expressed in prenatal and postnatal germ cells. In this study, we cloned and characterized the porcine Deleted in AZoospermia Like (pDAZL) gene. We found the full-length coding sequence of the pDAZL encoded a protein of 295 amino acids with a RNA recognition motif (amino acids 41-111) and a DAZ repeat (amino acids 167-120). The deduced protein sequence of pDAZL is 92.5% and 91.5% similar to those of human and bovine, respectively. PCR-MspI-RFLP and PCR-TaqI-RFLP were established to detect an A/G mutation in intron 7 and a C/A mutation in intron 9, respectively. Associations of two SNPs with litter size traits were assessed in Large White (n=275) and DIV (n=128) pig populations, and the statistical analysis demonstrated that CC produced 0.716 more (P<0.05) piglets born alive than CD genotypes in Large White pigs at TaqI locus (C/A mutation in intron 9), and the dominance effect was 0.304 pig per litter (P<0.05). This result suggests that the pDAZL gene might be a good candidate gene of litter size trait and provides some marker information for marker-assisted selection (MAS).
Zhang
Y H
YH
Key Laboratory of Pig Genetics and Breeding of Ministry of Agriculture, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, PR China.
Mei
S Q
SQ
Peng
X W
XW
Niu
B Y
BY
Ren
Z Q
ZQ
Zuo
B
B
Xu
D Q
DQ
Lei
M G
MG
Zheng
R
R
Jiang
S W
SW
Deng
C Y
CY
Xiong
Y Z
YZ
Li
F E
FE
D000818:Animals; D003001:Cloning, Molecular; D004252:DNA Mutational Analysis; D005787:Gene Frequency; D005819:Genetic Markers / Q000235:genetics / Q000502:physiology; D008098:Litter Size / Q000235:genetics; D008297:Male; D020641:Polymorphism, Single Nucleotide; D016601:RNA-Binding Proteins / Q000235:genetics* / Q000302:isolation & purification; D012098:Reproduction / Q000235:genetics; D034421:Sus scrofa / Q000235:genetics*
D005819:Genetic Markers; D016601:RNA-Binding Proteins