Direct electrogenerated chemiluminescence detection in high-performance liquid chromatography for determination of ofloxacin.
Ofloxacin (OFLX) exhibited strong electrogenerated chemiluminescence (ECL) in NaNO(3) solution with a dual-electrode system when constant current was exerted. Based on this observation, a sensitive direct ECL method coupled with high-performance liquid chromatography (HPLC) separation was developed for determination of OFLX in human serum. Factors affected the ECL emission were investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of OFLX in the range of 1.0x10(-8) to 4.0x10(-6) g mL(-1) and the detection limit was 4x10(-9) g mL(-1) (S/N=3). The proposed method was sensitive, simple and convenient to operate.
Sun
Yonghua
Y
College of Chemistry and Materials Science, Shaanxi Normal University, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Xi'an 710062, China.
Zhang
Zhujun
Z
Xi
Zhijun
Z
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D009566:Nitrates / Q000737:chemistry; D015242:Ofloxacin / Q000097:blood*; D012680:Sensitivity and Specificity
D009566:Nitrates; C031618:sodium nitrate; D015242:Ofloxacin