title: Sequence relations and coding properties of a subgenomic RNA isolated from barley stripe mosaic virus.
abstract: A subgenomic (SG) RNA ( approximately 800 nucleotides) is a minor component of barley stripe mosaic virus RNAs. The SG-RNAs isolated from the Type and North Dakota 18 (ND18) strains of BSMV have sequence homology with RNA 2 of the ("pseudo-two component") Type strain, which has two electrophoretic components, but only limited homology is evident with RNA 2 of the ND18 and Norwich strains, which have three electrophoretic components ("three component" strain). Instead, eDNAs from SG-RNA hybridize most efficiently with RNA 3 of the ND18 and Norwich strains. In wheat germ extracts the SG-RNAs direct the synthesis of two polypeptides with apparent molecular weights of 20 to 21 x 10(3). However, these two polypeptides were difficult to detect by polyacrylamide gel electrophoresis of extracts from Type- or ND18-infected barley and so appear not to accumulate during infection.
authors.affiliation: Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, United Kingdom.
authors.forename: A O
authors.initials: AO
authors.lastname: Jackson
authors.forename: J R
authors.initials: JR
authors.lastname: Dawson
authors.forename: S N
authors.initials: SN
authors.lastname: Covey
authors.forename: R
authors.initials: R
authors.lastname: Hull
authors.forename: J W
authors.initials: JW
authors.lastname: Davies
authors.forename: J E
authors.initials: JE
authors.lastname: McFarland
authors.forename: G D
authors.initials: GD
authors.lastname: Gustafson