title: A novel recombinant bacterial vaccine strain expressing dual viral antigens induces multiple immune responses to the Gag and gp120 proteins of HIV-1 in immunized mice.
abstract: Recombinant Salmonella enterica serovar Typhi can function as a live vector to deliver foreign antigens to the mammalian immune system and induce both mucosal and systemic immunity. In this study, we generated a recombinant Salmonella Typhi strain pilS-pilT-Gag+ (pVAX1-gp120) harboring the human immunodeficiency virus (HIV) gag gene integrated into the bacterial chromosome and gp120 gene carried by a plasmid. Mice inoculated with this recombinant bacterium through intranasal route produced high titers of IgG to gp120 in sera and IgA to gp120 in fecal washes. In addition, Gag-specific and gp120-specific cytotoxic T lymphocyte (CTL) responses were observed in sorted spleen lymphocytes of immunized mice. These results demonstrated that this recombinant Salmonella Typhi strain elicits multiple immune responses against both Gag and gp120 antigens of HIV, and thus would be a potential vaccine candidate to the prevention of HIV/AIDS.
authors.affiliation: State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wychang, Wuhan 430072, Hubei, PR China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Feng
authors.forename: Shiqun
authors.initials: S
authors.lastname: Wang
authors.forename: Fenglin
authors.initials: F
authors.lastname: Luo
authors.forename: Ying
authors.initials: Y
authors.lastname: Ruan
authors.forename: Lei
authors.initials: L
authors.lastname: Kang
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Xiang
authors.forename: Tao
authors.initials: T
authors.lastname: Chao
authors.forename: Guiqing
authors.initials: G
authors.lastname: Peng
authors.forename: Chengliang
authors.initials: C
authors.lastname: Zhu
authors.forename: Yongxin
authors.initials: Y
authors.lastname: Mu
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhu
authors.forename: Xiaolian
authors.initials: X
authors.lastname: Zhang
authors.forename: Jianguo
authors.initials: J
authors.lastname: Wu
mesh_terms: D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D012485:Salmonella typhi / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000821:virology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000821:virology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
chemical_list: D016915:AIDS Vaccines; D000914:Antibodies, Viral; D000956:Antigens, Viral; D015699:HIV Envelope Protein gp120; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D054301:gag Gene Products, Human Immunodeficiency Virus