[Construction and immunogenicity analysis of antigenic epitopes of swine influenza virus].
Several antigen epitopes were designed according to the sequences of Swine influenza virus hemagglutinin (HA) genes and lined with the interval. The gene was amplified by PCR and sub cloned into pET30a (+) vector. The fusion protein was expressed in E. coli BL21 (DE3) by induced with IPTG and purified by affinity chromatography. The molecular weight of the protein was about 20 kD in SDS-PAGE. Immunological activity of the fusion protein was analyzed by Western blot. The results showed that the fusion protein could interact with anti-His antibody and the rabbit antiserum against SIV. The immunized mouse can produced antibodies against the target peptide and HI antibody against SIV H1N1 or H3N2. This study provides a new vaccine candidate for SIV.
Liu
Huili
H
Institute of Animal Science and Veterinary Medicine, Shanghai Academy of Agriclutural Scinece, Shanghai 201106, China. huilil@163.com
Xing
Jilan
J
Pan
Jie
J
Yang
Qiufeng
Q
Zhao
Yanmin
Y
D000595:Amino Acid Sequence; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000096:biosynthesis / Q000235:genetics* / Q000276:immunology; D001483:Base Sequence; D000939:Epitopes / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D006388:Hemagglutinins / Q000235:genetics / Q000276:immunology; D006801:Humans; D007114:Immunization; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology*; D053122:Influenza A Virus, H3N2 Subtype / Q000235:genetics / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008969:Molecular Sequence Data; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
D000914:Antibodies, Viral; D000956:Antigens, Viral; D000939:Epitopes; D006388:Hemagglutinins; D011993:Recombinant Fusion Proteins