The inhibition of papaya mosaic virus assembly related to the effect of cations on its RNA.
Levels of MgCl2 and spermidine which have no effect on the equilibrium of papaya mosaic virus (PMV) coat protein, as measured by analytical centrifugation, or on its configuration, as judged by circular dichroism, inhibit reconstitution. This inhibition can be correlated with enhanced secondary structure of PMV-RNA as indicated by melting profiles. It is inferred that the inhibition of PMV assembly by NaCl or KCl results from the same effect, which is not found with glycine.
Abouhaidar
M
M
Department of Plant Sciences, University of Western Ontario, London, Ontario N6A 5B7, Canada.
Erickson
J W
JW
Bancroft
J B
JB