title: Defined media optimization for growth of recombinant Escherichia coli X90.
abstract: An optimized, defined minimal medium was developed to support balanced growth of Escherichia coli X90 harboring a recombinant plasmid. Foreign protein expression was repressed in these studies. A pulse injection technique was used to identify the growth responses to nutrients in a chemostat. Once the nutrients essential for growth had been identified, the yield coefficients for individual medium components. These yield coefficients were used to develop an optimized, glucose-limited defined minimal medium that supports balanced cell growth in chemostat culture. The biomass and substrate concentrations follow the Monod chemostat model. The maximum specific growth rate determined in a washout experiment is 0.87 h(-1) for this strain in the optimized medium. the glucose yield factor is 0.42 g DCW/g glucose and the maintenance coefficient is zero in the glucose-limited chemostat culture.
authors.affiliation: Department of Chemical Engineering, University of California at Berkeley, Berkeley, CA 94702, USA.
authors.forename: L
authors.initials: L
authors.lastname: Yee
authors.forename: H W
authors.initials: HW
authors.lastname: Blanch