Identification of a cryptic type III polyketide synthase (1,3,6,8-tetrahydroxynaphthalene synthase) from Streptomyces peucetius ATCC 27952.
We identified a 1,134-bp putative type III polyketide synthase from the sequence analysis of Streptomyces peucetius ATCC 27952, named Sp-RppA, which is characterized as 1,3,6,8-tetrahydroxynaphthalene synthase and shares 33% identity with SCO1206 from S. coelicolor A3(2) and 32% identity with RppA from S. griseus. The 1,3,6,8-tetrahydroxynaphthalene synthase is known to catalyze the sequential decarboxylative condensation, intramolecular cyclization, and aromatization of an oligoketide derived from five units of malonyl-CoA to give 1,3,6,8-tetrahydroxynaphthalene, which spontaneously oxidizes to form 2,5,7-trihydroxy-1,4-naphthoquinone (flaviolin). In this study, we report the in vivo expression and in vitro synthesis of flaviolin from purified gene product (Sp-RppA).
Ghimire
Gopal Prasad
GP
Institute of Biomolecule Reconstruction, Department of Pharmaceutical Engineering, SunMoon University, Asan, Korea.
Oh
Tae-Jin
TJ
Liou
Kwangkyoung
K
Sohng
Jae Kyung
JK
D000217:Acyltransferases / Q000737:chemistry / Q000302:isolation & purification*; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D008969:Molecular Sequence Data; D009284:Naphthols / Q000737:chemistry; D009285:Naphthoquinones / Q000737:chemistry; D010641:Phenotype; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D021241:Spectrometry, Mass, Electrospray Ionization; D013302:Streptomyces / Q000201:enzymology*
C121288:1,3,6,8-tetrahydroxynaphthalene; D009284:Naphthols; D009285:Naphthoquinones; C041659:flaviolin; D000217:Acyltransferases; C020141:flavanone synthetase