title: Continuous cell-free protein synthesis using glycolytic intermediates as energy sources.
abstract: In this work, we demonstrate that glycolytic intermediates can serve as efficient energy sources to regenerate ATP during continuous-exchange cell-free (CECF) protein synthesis reactions. Through the use of an optimal energy source, approximately 10 mg/ml of protein was generated from CECF protein synthesis reaction at greatly reduced reagent costs. Compared with the conventional reactions utilizing phosphoenol pyruvate as an energy source, the described method yields 10-fold higher productivity per unit reagent cost, making the techniques of CECF protein synthesis more realistic alternative for rapid protein production.
authors.affiliation: Department of Fine Chemical Engineering and Chemistry, Chungnam National University, Daejeon 305-764, Korea.
authors.forename: Ho-Cheol
authors.initials: HC
authors.lastname: Kim
authors.forename: Tae-Wan
authors.initials: TW
authors.lastname: Kim
authors.forename: Chang-Gil
authors.initials: CG
authors.lastname: Park
authors.forename: In-Seok
authors.initials: IS
authors.lastname: Oh
authors.forename: Kyungmoon
authors.initials: K
authors.lastname: Park
authors.forename: Dong-Myung
authors.initials: DM
authors.lastname: Kim
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D001674:Bioelectric Energy Sources / Q000382:microbiology*; D004736:Energy-Generating Resources; D004926:Escherichia coli / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D006019:Glycolysis; D014176:Protein Biosynthesis
chemical_list: D000255:Adenosine Triphosphate; D005947:Glucose