[Effect of c-ski on biological behavior of rat skin-derived fibroblast].
OBJECTIVE
To observe the effect of oncogene c-ski on the cell viability, myofibroblast differentiation and type I collagen secretion of rat skin-derived fibroblast.


METHODS
In situ expression of c-Ski in fibroblast was detected by immunohistochemistry. After cells transfected with c-ski gene, the changes of cell proliferation was measured with the method of MTT, the type I collagen secretion and myofibroblast differentiation were checked with the methods of immunohistochemistry and RT-PCR.


RESULTS
There was obvious expression of c-Ski in fibroblast, which mainly located in nucleus. c-ski could promote cell proliferation in a dose-dependent manner, and also decrease mRNA and protein expression of type I collagen, but had no effect on myofibrblast differentiation.


CONCLUSIONS
The roles of c-ski promoting cell viability and decreasing collagen secretion may have an important significance in accelerating wound healing and reducing scar formation.
Liu
Xia
X
Molecular Biology Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing 400042, China.
Li
Ping
P
Zhang
En
E
Liu
Ping
P
Zhou
Yuan-Guo
YG
D000818:Animals; D049109:Cell Proliferation; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D007150:Immunohistochemistry; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012867:Skin / Q000166:cytology / Q000378:metabolism*
D024042:Collagen Type I; D011518:Proto-Oncogene Proteins; C525545:Ski protein, rat