Sequence relations and coding properties of a subgenomic RNA isolated from barley stripe mosaic virus.
A subgenomic (SG) RNA ( approximately 800 nucleotides) is a minor component of barley stripe mosaic virus RNAs. The SG-RNAs isolated from the Type and North Dakota 18 (ND18) strains of BSMV have sequence homology with RNA 2 of the ("pseudo-two component") Type strain, which has two electrophoretic components, but only limited homology is evident with RNA 2 of the ND18 and Norwich strains, which have three electrophoretic components ("three component" strain). Instead, eDNAs from SG-RNA hybridize most efficiently with RNA 3 of the ND18 and Norwich strains. In wheat germ extracts the SG-RNAs direct the synthesis of two polypeptides with apparent molecular weights of 20 to 21 x 10(3). However, these two polypeptides were difficult to detect by polyacrylamide gel electrophoresis of extracts from Type- or ND18-infected barley and so appear not to accumulate during infection.
Jackson
A O
AO
Department of Virus Research, John Innes Institute, Colney Lane, Norwich NR4 7UH, United Kingdom.
Dawson
J R
JR
Covey
S N
SN
Hull
R
R
Davies
J W
JW
McFarland
J E
JE
Gustafson
G D
GD