Cloning and characterization of the tiger shrimp lysozyme.
Lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections. A lysozyme gene isolated from tiger shrimp, Penaeus monodon, was cloned, sequenced and characterized. The cDNA consists of a signal peptide of 18 amino acids and a mature peptide of 140 amino acids. The lysozyme is presumed to be a chicken-type lysozyme for it possesses two catalytic sites and eight cysteine residues which are highly conserved across species of chicken-type lysozymes. The lysozyme cDNAs of Penaeus semisulcatus, Litopenaeus vannamei, Macrobrachium nipponense and Macrobrachium rosenbergii were also cloned. High similarities existed among shrimp and prawn lysozymes but phylogenetic relationship of shrimps and prawns based on lysozyme molecules did not quite consistent with traditional taxonomic classification. High mRNA expression was detected in hepatopancreas, haemocytes and gill of tiger shrimp. Recombinant lysozyme exhibited potent lytic activities against fish pathogens providing evidence of the involvement of lysozyme in shrimp immunity.
Ye
Xing
X
Key Laboratory of Tropical & Subtropical FishBreeding & Cultivation of CAFS, Pearl River Fisheries Research Institute, Xing Yu Road, Guangzhou, 510380, China. gzyexing@163.com
Gao
Feng-Ying
FY
Zheng
Qing-Mei
QM
Bai
Jun-Jie
JJ
Wang
Huan
H
Lao
Hai-Hua
HH
Jian
Qing
Q
D000818:Animals; D001483:Base Sequence; D020134:Catalytic Domain; D003001:Cloning, Molecular / Q000379:methods*; D003545:Cysteine; D018076:DNA, Complementary; D008969:Molecular Sequence Data; D009113:Muramidase / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D033561:Penaeidae / Q000201:enzymology* / Q000276:immunology; D010802:Phylogeny; D012333:RNA, Messenger / Q000032:analysis; D017421:Sequence Analysis; D014018:Tissue Distribution
D018076:DNA, Complementary; D012333:RNA, Messenger; D009113:Muramidase; D003545:Cysteine