title: Gene expression during liver regeneration after partial hepatectomy in mice lacking type 1 tumor necrosis factor receptor.
abstract: BACKGROUND
  To investigate the function of tumor necrosis factor-alpha (TNF-alpha) during hepatocyte proliferation, we studied liver regeneration following partial hepatectomy in mice lacking type 1 TNF receptor (TNFR-1).
  
  
  METHODS
  TNFR-1 knockout (KO) and wild-type mice were subjected to partial (two-thirds) hepatectomy. Liver regeneration was evaluated by assessing liver weights and Ki67 immunohistochemistry. Riken complementary DNA microarray analysis was performed for liver samples from mice undergoing partial hepatectomy to better compare different mouse partial hepatectomy models (TNFR-1 KO mice, KO group; and wild-type mice, W group).
  
  
  RESULTS
  Liver weight was regained after 14 days in the KO group, and after 7 days in the W group. Genes including lipopolysaccharide, toll-like receptor 4 precursor, mitogen-activated protein kinase kinase kinase 4, mitogen-activated protein kinase kinase kinase kinase 4, and mitogen-activated protein kinase 8-interacting protein were up-regulated in the KO group. As for the cell-cycle-regulated genes, the levels of cyclin D1, nuclear factor-kappa B light chain, and TNF receptor super family membrane 1a were down-regulated in the KO group. Microarray analysis showed decreased activities of the hexokinase- and phospho-fructokinase-related glycolytic pathways in the KO group.
  
  
  CONCLUSIONS
  These results contribute to the better understanding of the mechanisms of liver regeneration after partial hepatectomy in TNFR-1 KO mice.
authors.affiliation: Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Shimizu
authors.forename: Shinji
authors.initials: S
authors.lastname: Togo
authors.forename: Takahumi
authors.initials: T
authors.lastname: Kumamoto
authors.forename: Hirochika
authors.initials: H
authors.lastname: Makino
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Morita
authors.forename: Kuniya
authors.initials: K
authors.lastname: Tanaka
authors.forename: Toru
authors.initials: T
authors.lastname: Kubota
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Ichikawa
authors.forename: Youji
authors.initials: Y
authors.lastname: Nagasima
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Okazaki
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Hayashizaki
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Shimada
mesh_terms: D000410:Alanine Transaminase / Q000097:blood; D000469:Alkaline Phosphatase / Q000097:blood; D000818:Animals; D002453:Cell Cycle / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D005786:Gene Expression Regulation; D006498:Hepatectomy / Q000379:methods* / Q000401:mortality; D007150:Immunohistochemistry; D015850:Interleukin-6 / Q000097:blood; D019394:Ki-67 Antigen / Q000032:analysis; D008099:Liver / Q000166:cytology / Q000276:immunology; D008115:Liver Regeneration / Q000235:genetics* / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000235:genetics / Q000302:isolation & purification; D047888:Receptors, Tumor Necrosis Factor, Type I / Q000172:deficiency*; D015996:Survival Rate
chemical_list: D018076:DNA, Complementary; D015850:Interleukin-6; D019394:Ki-67 Antigen; D047888:Receptors, Tumor Necrosis Factor, Type I; D012313:RNA; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase