Identification of the 30K protein of TMV by immunoprecipitation with antibodies directed against a synthetic peptide.
A synthetic hexadecapeptide corresponding to the predicted C-terminal sequence of the 30K protein of TMV has been coupled to bovine serum albumin and used to raise antibodies in rabbits. The resulting antiserum reacted with the 30K protein translated in vitro. We report the use of this antiserum in the first detection of the 30K protein in vivo, in TMV-infected tobacco protoplasts. Several proteins, the so called family of 30K-related peptides, were immunoprecipitated among in vitro translation products, but only the 30K protein was immunoprecipitated from TMV-infected protoplasts.
Ooshika
I
I
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo, Tokyo 113, Japan.
Watanabe
Y
Y
Meshi
T
T
Okada
Y
Y
Igano
K
K
Inouye
K
K
Yoshida
N
N