title: [Preparation and distribution of polyclonal antibodies against human PIWIL3 protein in tumor tissues].
abstract: OBJECTIVE
  To prepare specific antibodies against human PIWIL3 protein of Argonaute family and study its expression and distribution in human tumors.
  
  
  METHODS
  To synthesize the polypeptide using the optimal polypeptide immunogens based on sequence homology and peptide immunogenicty in the internal polypeptide selecting database. The synthesized polypeptide were combined with KLH, then immunized the experimental rabbits to produce antiserum. The antiserum was purified by gels custodited the polypeptide using affinity chromatograph, and validated its ability to combine with the corresponding proteins by enzyme-linked immunosorbentassay (ELISA) and Western blot. The expression and distribution of PIWIL3 protein were detected by human tumor tissue biochips.
  
  
  RESULTS
  The specific antibodies against human PIWIL3 proteins were prepared and had high purity. PIWIL3 proteins were expressed in the cytoplasma of human astrocytic glioma and meningioma.
  
  
  CONCLUSIONS
  The specific antibodies against human PIWIL3 protein have been successfully prepared by using the internal polypeptide selecting database to obtain the optimal polypeptide immunogens, which are distinguished from other sequence homology proteins in the subfamily. These antibodies will have potential value for human PIWIL3 protein in human specific tumor pathogenesis.
authors.affiliation: Department of Gastroenternology, Tongji Hospital, Tongji University, Shanghai 200065, China. xlwang2006@163.com
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Wang
authors.forename: Xi-Mei
authors.initials: XM
authors.lastname: Chen
authors.forename: Heng-Jun
authors.initials: HJ
authors.lastname: Gao
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Huang
mesh_terms: D000906:Antibodies / Q000276:immunology*; D001254:Astrocytoma / Q000378:metabolism; D006801:Humans; D008579:Meningioma / Q000378:metabolism; D010455:Peptides / Q000138:chemical synthesis / Q000737:chemistry / Q000276:immunology; D011506:Proteins / Q000737:chemistry / Q000276:immunology*
chemical_list: D000906:Antibodies; D010455:Peptides; D011506:Proteins