title: Enzymatic synthesis of 2-beta-D-galactopyranosyloxy ethyl methacrylate (GalEMA) by the thermophilic archeon Sulfolobus solfataricus.
abstract: beta-Glycosidases catalyze the synthesis of glycosides when a nucleophilic acceptor other than water is present in the reaction medium. We describe the enzymatyc synthesis of 2-beta-D-galactopyranosyloxyethyl methacrylate (GalEMA) starting from 2-hydroxyethyl methacrylate (HEMA) and p-nitrophenyl-beta-D-galactopyranoside (pNPG) using a beta-glycosidase activity present in the thermophilic archaeon Sulfolobus solfataricus. This thermophilic enzyme catalyzes the transfer of the galactopyranosyl unit from pNPG to the HEMA hydroxyl group by the formation of a new beta-glycosidic bond. The conditions of the biocatalytic system have been optimized to obtain high yield of GalEMA. (c) 1996 John Wiley & Sons, Inc.
authors.affiliation: Istituto di Biochimica delle Proteine ed Enzimologia, C. N. R., Via Toiano 6, 80072 Arco Felice, Naples, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Santin
authors.forename: F
authors.initials: F
authors.lastname: Rosso
authors.forename: A
authors.initials: A
authors.lastname: Sada
authors.forename: G
authors.initials: G
authors.lastname: Peluso
authors.forename: R
authors.initials: R
authors.lastname: Improta
authors.forename: A
authors.initials: A
authors.lastname: Trincone