Hepatoprotective activity of Woodfordia fruticosa Kurz flowers against carbon tetrachloride induced hepatotoxicity.
BACKGROUND
Dried flowers of Woodfordia fruticosa Kurz. Family Lythraceae are used in variety of diseases in traditional Indian system of medicine including hepatic ailments.


OBJECTIVE
The aim of present study was to validate hepatoprotective activity of flowers of Woodfordia fruticosa Kurz.


METHODS
Petroleum ether (WF1), chloroform (WF2), ethyl alcohol (WF3) and aqueous (WF4) extracts of the flowers of Woodfordia fruticosa were evaluated for hepatoprotective activity against carbon tetrachloride induced hepatotoxicity using biochemical markers, hexobarbitone sleep time, bromosulphalein (BSP) clearance test and effect on bile flow and bile solids.


RESULTS
The aqueous extract (WF4) was most potent among the four extracts studied in detail. WF4 showed significant hepatoprotective activity against carbon tetrachloride induced hepatotoxicity as evident by restoration of serum transaminases, alkaline phosphatase, bilirubin and triglycerides. The restoration of microsomal aniline hydroxylase and amidopyrine-N-demethylase activities indicated the improvement in functional status of endoplasmic reticulum. Restoration of lipid peroxidation and glutathione contents suggests the antioxidant property of WF4. The recovery in bromosulphalein clearance and stimulation of bile flow suggested the improved excretory and secretary capacity of hepatocytes. Light microscopy of the liver tissue further confirmed the reversal of damage induced by hepatotoxin.


CONCLUSIONS
Present study showed that the aqueous extract of Woodfordia fruticosa significantly restores physiological integrity of hepatocytes. WF4 did not show any sign of toxicity up to oral dose of 2g/kg in mice.
Chandan
B K
BK
Indian Institute of Integrative Medicine, Canal Road, Jammu-Tawi 180016, J&K, India. balbkchandan@rediffmail.com
Saxena
A K
AK
Shukla
Sangeeta
S
Sharma
N
N
Gupta
D K
DK
Singh
K
K
Suri
Jyotsna
J
Bhadauria
M
M
Qazi
G N
GN
D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage* / Q000302:isolation & purification / Q000633:toxicity; D002251:Carbon Tetrachloride; D004305:Dose-Response Relationship, Drug; D005260:Female; D035264:Flowers; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D022781:Hepatocytes / Q000187:drug effects / Q000378:metabolism; D007194:India; D015227:Lipid Peroxidation / Q000187:drug effects; D008107:Liver Diseases / Q000188:drug therapy*; D008111:Liver Function Tests; D008297:Male; D008519:Medicine, Traditional; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage* / Q000633:toxicity; D051381:Rats; D017208:Rats, Wistar; D012997:Solvents / Q000737:chemistry; D031565:Woodfordia / Q000737:chemistry*
D000975:Antioxidants; D010936:Plant Extracts; D012997:Solvents; D002251:Carbon Tetrachloride; D005978:Glutathione