title: Seed defensins of barnyard grass Echinochloa crusgalli (L.) Beauv.
abstract: From the annual weed barnyard grass Echinochloa crusgalli (L.) Beauv., two novel defensins Ec-AMP-D1 and Ec-AMP-D2 that differ by a single amino acid substitution were isolated by a combination of different chromatographic procedures. Both defensins were active against several phytopathogenic fungi and the oomycete Phytophthora infestans at micromolar concentrations. The Ec-AMP-D1 showed higher activity against the oomycete than Ec-AMP-D2. The comparison of the amino acid sequences of the antifungal E. crusgalli defensins with those of earlier characterized T. kiharae defensins [T.I. Odintsova, Ts.A. Egorov, A.Kh. Musolyamov, M.S. Odintsova, V.A. Pukhalsky, E.V. Grishin, Seed defensins from T. kiharae and related species: genome localization of defensin-encoding genes, Biochimie, 89 (2007) 605-612.] that were devoid of substantial antifungal activity point to the C-terminal region of the molecule as the main determinant of the antifungal activity of E. crusgalli defensins.
authors.affiliation: Laboratory of Plant Genetics, Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, Russian Federation.
authors.forename: Tatyana I
authors.initials: TI
authors.lastname: Odintsova
authors.forename: Eugene A
authors.initials: EA
authors.lastname: Rogozhin
authors.forename: Yurij
authors.initials: Y
authors.lastname: Baranov
authors.forename: Alexander Kh
authors.initials: AKh
authors.lastname: Musolyamov
authors.forename: Nasser
authors.initials: N
authors.lastname: Yalpani
authors.forename: Tsezi A
authors.initials: TA
authors.lastname: Egorov
authors.forename: Eugene V
authors.initials: EV
authors.lastname: Grishin
mesh_terms: D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000890:Anti-Infective Agents / Q000302:isolation & purification* / Q000494:pharmacology*; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology; D023082:Defensins / Q000235:genetics / Q000302:isolation & purification* / Q000494:pharmacology*; D031747:Echinochloa / Q000737:chemistry*; D005658:Fungi / Q000187:drug effects / Q000378:metabolism; D008969:Molecular Sequence Data; D055750:Phytophthora infestans / Q000187:drug effects / Q000378:metabolism; D012639:Seeds / Q000737:chemistry; D016415:Sequence Alignment
chemical_list: D000890:Anti-Infective Agents; D000935:Antifungal Agents; D023082:Defensins