Dose-response relationship analysis of vigabatrin doses and their antinociceptive effects in the hot-plate test in mice.
This study was aimed at determining the analgesic effects of vigabatrin (VGB, a newer antiepileptic drug) in the acute thermal pain model (hot-plate test) in mice. Linear regression analysis was used to evaluate a dose-response relationship between logarithms of VGB doses and their resultant maximum possible antinociceptive effects (MPAE) in the hot-plate test in mice. From the linear equation of dose-response relationship, doses of VGB that increased the antinociceptive effect by 15%, 20% and 25% were calculated and amounted in this study to 144, 383 and 1,016 mg/kg, respectively. In conclusion, VGB in a dose-dependent manner produces the analgesic effects in mice in the hot-plate test. The method allowing for the calculation of doses of VGB increasing the antinociceptive effects by 15%, 20% and 25% can be readily adapted to preclinical studies because these values perfectly characterize the potency of antiepileptic drugs with respect to suppression of acute thermal pain in mice.
Luszczki
Jarogniew J
JJ
Department of Pathophysiology, Medical University of Lublin, Jaczewskiego 8, PL 20-090 Lublin, Poland. jarogniew.luszczki@am.lublin.pl
Czuczwar
Stanisław J
SJ
D000700:Analgesics / Q000494:pharmacology* / Q000627:therapeutic use; D000818:Animals; D000927:Anticonvulsants / Q000494:pharmacology / Q000627:therapeutic use; D004305:Dose-Response Relationship, Drug; D006358:Hot Temperature; D006930:Hyperalgesia / Q000188:drug therapy / Q000209:etiology; D016014:Linear Models; D008297:Male; D051379:Mice; D010146:Pain / Q000188:drug therapy* / Q000209:etiology; D010147:Pain Measurement / Q000379:methods*; D020888:Vigabatrin / Q000494:pharmacology* / Q000627:therapeutic use
D000700:Analgesics; D000927:Anticonvulsants; D020888:Vigabatrin