title: Satellite RNAs of cucumber mosaic virus: characterization of two new satellites.
abstract: Two new satellite RNAs of cucumber mosaic virus (CMV) which did not induce necrosis on tomato in the presence of CMV, B-sat RNA, and WL-sat RNA, were shown to be related by sequence to two well-characterized satellite RNAs of CMV: G-sat RNA (non-necrotic on tomato) and n-CARNA 5 (necrotic on tomato). Using the techniques of molecular hybridization analysis, RNA fingerprinting and partial RNA sequencing, B-sat RNA and WL-sat RNA were shown to be more closely related to each other (probably differing by only a small number of nucleotides) than to the other two satellite RNAs. Furthermore, B-sat RNA and WL-sat RNA showed greater sequence homology with G-sat RNA than with n-CARNA 5. WL-sat RNA, which induces a "white-leaf" disease on tomato in the presence of CMV [Gonsalves et al. (1982)., exhibited heterogeneity of sequence in at least one nucleotide position.
authors.affiliation: Department of Plant Pathology, Cornell University, Ithaca, New York 14853, USA.
authors.forename: P
authors.initials: P
authors.lastname: Palukaitis
authors.forename: M
authors.initials: M
authors.lastname: Zaitlin