Quantification of DNA in biologic scaffold materials.
Biological scaffold materials composed of extracellular matrix (ECM) are routinely used for a variety of clinical applications ranging from the treatment of chronic skin ulcers to hernia repair and orthopaedic soft tissue reconstruction. The tissues and species from which the ECM is harvested vary widely as do the methods used to remove the cellular component of the source tissues. The efficacy of decellularization procedures can be quantified by examination of the DNA that remains in the ECM. The objective of the present study was to determine the DNA content and fragment length in both laboratory produced and commercially available ECM scaffold materials. Results showed that the majority of DNA is removed from ECM devices but that small amounts remained in most tested materials.
Gilbert
Thomas W
TW
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA.
Freund
John M
JM
Badylak
Stephen F
SF
D000818:Animals; D004247:DNA / Q000032:analysis*; D004801:Eosine Yellowish-(YS); D005109:Extracellular Matrix / Q000737:chemistry*; D006416:Hematoxylin; D006801:Humans; D007211:Indoles; D009930:Organic Chemicals; D013552:Swine; D054457:Tissue Scaffolds / Q000737:chemistry* / Q000592:standards
D007211:Indoles; D009930:Organic Chemicals; C099476:PicoGreen; C007293:DAPI; D004247:DNA; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin