Proteomics of multigenic families from species underrepresented in databases: the case of loquat (Eriobotrya japonica Lindl.) polyphenol oxidases.
Here, we approach the problem of obtaining accurate and reliable information about the gene origin of a protein belonging to a multigenic family, polyphenol oxidase, from an underrepresented species, Eriobotrya japonica. De novo sequencing was a key approach to obtain broad sequence coverage. Alignment of peptides on their most similar homologous protein revealed divergent amino acid positions that lead to hypothesize the minimal number of genes encoding for the proteins analyzed.
Sellés-Marchart
Susana
S
Grupo de Proteomica y Genomica Funcional de Plantas, Departamento de Agroquimica y Bioquimica, Facultad de Ciencias, Universidad de Alicante, Spain.
Luque
Ignacio
I
Casado-Vela
Juan
J
Martínez-Esteso
Maria José
MJ
Bru-Martínez
Roque
R
D000595:Amino Acid Sequence; D000917:Antibody Formation; D015153:Blotting, Western; D004156:Catechol Oxidase / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D030562:Databases, Protein; D004591:Electrophoresis, Polyacrylamide Gel; D031983:Eriobotrya / Q000201:enzymology*; D008969:Molecular Sequence Data; D005810:Multigene Family; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D012995:Solubility
D004156:Catechol Oxidase