title: Detection of the A189G mtDNA heteroplasmic mutation in relation to age in modern and ancient bones.
abstract: The aim of this study was to demonstrate the presence of the A189G age-related point mutation on DNA extracted from bone. For this, a peptide nucleic acid (PNA)/DNA sequencing method which can determine an age threshold for the appearance of the mutation was used. Initially, work was done in muscle tissue in order to evaluate the sensitivity of the technique and afterwards in bone samples from the same individuals. This method was also applied to ancient bones from six well-preserved skeletal remains. The mutation was invariably found in muscle, and at a rate of up to 20% in individuals over 60 years old. In modern bones, the mutation was detected in individuals aged 38 years old or more, at a rate of up to 1%, but its occurrence was not systematic (only four out of ten of the individuals over 50 years old carried the heteroplasmy). For ancient bones, the mutation was also found in the oldest individuals according to osteologic markers. The study of this type of age-related mutation and a more complete understanding of its manifestation has potentially useful applications. Combined with traditional age markers, it could improve identification accuracy in forensic cases or in anthropological studies of ancient populations.
authors.affiliation: CNRS, FRE 2960, Laboratoire d'Anthropobiologie, 37 allées Jules Guesde, 31073, Toulouse, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Lacan
authors.forename: Catherine
authors.initials: C
authors.lastname: Thèves
authors.forename: Sylvain
authors.initials: S
authors.lastname: Amory
authors.forename: Christine
authors.initials: C
authors.lastname: Keyser
authors.forename: Eric
authors.initials: E
authors.lastname: Crubézy
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Salles
authors.forename: Bertrand
authors.initials: B
authors.lastname: Ludes
authors.forename: Norbert
authors.initials: N
authors.lastname: Telmon
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D015139:Blotting, Southern; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000473:pathology; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012272:Ribs / Q000473:pathology*; D017422:Sequence Analysis, DNA / Q000379:methods
chemical_list: D004272:DNA, Mitochondrial