Potential gene flow in natural populations of the Drosophila ananassae species cluster inferred from a nuclear mitochondrial pseudogene.
A pseudogene with 94% similarity to mitochondrial cytochrome c oxidase subunit I (COI) was identified and localized to chromosome 4 of Drosophila ananassae. Because this chromosome is believed to have reduced recombination, its history can be traced using the pseudo-COI sequence. Pseudo-COI sequences were obtained from 27 iso-female lines of six taxa belonging to the D. ananassae species cluster in which reproductive isolation is incomplete. The phylogenetic network constructed from seven recognized haplotypes (#0-#6) indicated that different taxa inhabiting the same geographic area share the haplotypes: #1 from Papua New Guinean populations of D. ananassae and pallidosa-like-Wau; #2 from Papua New Guinean populations of D. ananassae, pallidosa-like, and papuensis-like; and #4 from South Pacific populations of D. ananassae and D. pallidosa. Taxon-K has a unique haplotype (#6), and 18 mutation steps separate it from the closest haplotype, #2. We discuss the possibility of chromosome 4 introgression beyond taxon boundaries.
Sawamura
Kyoichi
K
Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan. sawamura@biol.tsukuba.ac.jp
Koganebuchi
Kae
K
Sato
Hajime
H
Kamiya
Koichi
K
Matsuda
Muneo
M
Oguma
Yuzuru
Y
D000818:Animals; D001483:Base Sequence; D002467:Cell Nucleus / Q000235:genetics*; D002875:Chromosomes / Q000648:ultrastructure; D004272:DNA, Mitochondrial / Q000235:genetics*; D004330:Drosophila / Q000235:genetics*; D005260:Female; D051456:Gene Flow; D005843:Geography / Q000379:methods; D008297:Male; D008969:Molecular Sequence Data; D010219:Papua New Guinea; D010802:Phylogeny; D011544:Pseudogenes; D013045:Species Specificity
D004272:DNA, Mitochondrial