title: Synthetic Studies in Phytochrome Chemistry.
abstract: An account is given of the author's several approaches to the synthesis of the parent chromophore of phytochrome (1), a protein-bound linear tetrapyrrole derivative that controls photomorphogenesis in higher plants. These studies culminated in enantioselective syntheses of both 2R- and 2S-phytochromobilin (4), as well as several (13)C-labeled derivatives designed to probe the site of Z,E-isomerization during photoexcitation. When reacted in vitro, synthetic 2R-4 and recombinant-derived phytochrome apoprotein N-C produced a protein-bound chromophore with identical difference spectra to naturally occurring 1.
authors.affiliation: Department of Chemistry, Dartmouth College, Hanover, New Hampshire 03755, USA.
authors.forename: Peter A
authors.initials: PA
authors.lastname: Jacobi
authors.forename: Imad M
authors.initials: IM
authors.lastname: Adel Odeh
authors.forename: Subhas C
authors.initials: SC
authors.lastname: Buddhu
authors.forename: Guolin
authors.initials: G
authors.lastname: Cai
authors.forename: Sundaramoorthi
authors.initials: S
authors.lastname: Rajeswari
authors.forename: Douglas
authors.initials: D
authors.lastname: Fry
authors.forename: Wanjun
authors.initials: W
authors.lastname: Zheng
authors.forename: Robert W
authors.initials: RW
authors.lastname: Desimone
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Guo
authors.forename: Lisa D
authors.initials: LD
authors.lastname: Coutts
authors.forename: Sheila I
authors.initials: SI
authors.lastname: Hauck
authors.forename: Sam H
authors.initials: SH
authors.lastname: Leung
authors.forename: Indranath
authors.initials: I
authors.lastname: Ghosh
authors.forename: Douglas
authors.initials: D
authors.lastname: Pippin