Development of SM5-1-conjugated ultrasmall superparamagnetic iron oxide nanoparticles for hepatoma detection.
Ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles are maghemite or magnetite nanoparticles currently used as contrast agent in magnetic resonance imaging (MRI). In this study, a targeted contrast agent (SM-USPIO) was prepared by conjugating coprecipitated USPIO to a humanized SM5-1 antibody which can specifically react with human hepatocellular carcinoma (HCC) cells. The binding and internalization of SM-USPIO to the HCC cell line ch-hep-3 was confirmed by flow cytometry and confocal microscopy. Furthermore, SM-USPIO was demonstrated to be able to selectively accumulate in the tumor cells, resulting in a marked decrease of MRI T2-weighted signal intensity. Biodistribution studies demonstrated the efficient accumulation of SM-USPIO in the ch-hep-3 tumor in nude mice. The in vivo study in the ch-hep-3 tumor-bearing nude mice indicated that MRI using the SM-USPIO as contrast agent possessed good diagnostic ability, suggesting that SM-USPIO had the potential to be a promising targeted contrast agent for diagnosis of HCC.
Kou
Geng
G
International Joint Cancer Institute, The Second Military Medical University, 800 Xiang Yin Road, New Building 10-11th Floor, Shanghai 200433, People's Republic of China.
Wang
Shuhui
S
Cheng
Changming
C
Gao
Jie
J
Li
Bohua
B
Wang
Hao
H
Qian
Weizhu
W
Hou
Sheng
S
Zhang
Dapeng
D
Dai
Jianxin
J
Gu
Hongchen
H
Guo
Yajun
Y
D000911:Antibodies, Monoclonal / Q000276:immunology; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000378:metabolism; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000378:metabolism*; D003911:Dextrans; D005290:Ferric Compounds / Q000378:metabolism; D052203:Ferrosoferric Oxide; D006801:Humans; D007501:Iron / Q000378:metabolism*; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000378:metabolism; D008279:Magnetic Resonance Imaging; D058185:Magnetite Nanoparticles; D053758:Nanoparticles; D010087:Oxides / Q000378:metabolism*
D000911:Antibodies, Monoclonal; D003287:Contrast Media; D003911:Dextrans; D005290:Ferric Compounds; D058185:Magnetite Nanoparticles; D010087:Oxides; C097921:ferumoxtran-10; C000499:ferric oxide; D007501:Iron; D052203:Ferrosoferric Oxide