An evaluation of bacterial contamination of barriers used in periapical tissue regeneration: Part 2--Bacterial penetration.
OBJECTIVE
To compare the relative penetration of Prevotella melaninogenica and Enterococcus faecalis through 3 guided tissue regeneration membranes: Atrisorb, Lambone, and OsseoQuest. It was hypothesized that OsseoQuest would show increased bacterial penetration when compared to Lambone and Atrisorb.


METHODS
Centrifuge tubes containing trypticase soy broth were sealed with circular sections of membranes and placed in test tubes containing culture media. The bacterial penetration was assessed by passage of bacteria from the outer tube culture media to the inner centrifuge tube media through the membrane. After incubation for 4 and 48 hours, the media from the outer and inner tubes were compared for bacterial count.


RESULTS
P melaninogenica exhibited 91% penetration for Lambone in 2 days, while OsseoQuest displayed 87% penetration with E faecalis in the same time. Atrisorb displayed a minimal penetration with both bacteria (2%).


CONCLUSIONS
Atrisorb displayed the least bacterial penetration, which may be attributed to membrane structure, chemical configuration, hydrophobicity, and porosity of tested membranes.
Sharma
Priya
P
Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland, Baltimore, MD 21201, USA. PChand@umaryland.edu
Mickel
André K
AK
Chogle
Sami
S
Sharma
Prem Nath
PN
Han
Yiping W
YW
Jones
Jefferson J
JJ
D020341:Absorbable Implants / Q000382:microbiology*; D013293:Enterococcus faecalis; D004866:Equipment Contamination; D016557:Guided Tissue Regeneration, Periodontal; D008567:Membranes, Artificial; D010539:Permeability; D016062:Porosity; D001443:Prevotella melaninogenica
D008567:Membranes, Artificial