title: Cell junctions in the endocrine dorsal body cells of Helisoma duryi (Mollusca: Pulmonata): an in vitro study.
abstract: The endocrine dorsal bodies of gastropod molluscs regulate reproduction and are closely associated with the central nervous system. Previous studies on Helisoma duryi have shown that the dorsal body cells of reproducing snails contain more gap junctions than those of non-reproducing virgin snails. More dorsal body cells were isolated from virgin snails than reproducing snails in Ca(2+) and Mg(2+) free experimental saline. The isolated spherical cells attached to the culture dish, spread and formed filopodium-like processes within a few hours in culture medium containing Ca(2+) and Mg(2+). Many isolated cells reaggregated after 4-6 h in culture forming septate junction-like and gap junction-like cell contacts, as revealed by thin section and freeze-fracture studies. Following 10 min incubation in carboxyfluorescein diacetate the isolated cells fluoresced and, after aggregation, these cells transferred fluorescent dye to unlabelled cells. Cell aggregation was inhibited by cytochalasin D. Staining by NBD-phallacidin revealed the presence of actin in the filopodium-like processes of spread cells and in the perinuclear cytoplasm. It is likely that the septate junction-like contacts provide sites of cell attachment between aggregating cells; gap junctions are involved in intercellular communication, and actin is required in this process.
authors.affiliation: Department of Biology, York University, North York, Ontario, M3J 1P3, Canada.
authors.forename: H R
authors.initials: HR
authors.lastname: Khan
authors.forename: R R
authors.initials: RR
authors.lastname: Shivers
authors.forename: A S
authors.initials: AS
authors.lastname: Saleuddin