title: Synthesis and gene transfection efficacies of PEI-cholesterol-based lipopolymers.
abstract: Nine lipopolymers based on low molecular weight polyethyleneimines (PEI) and cholesterol via an ether linkage between the polymer amine and the cholesterol backbone have been synthesized. Different percentage of cholesterol moieties have been grafted on three types of PEI of molecular weights 800, 1200, and 2000. These lipopolymers were studied for gene transfection activities in HeLa cells. All the lipopolymers were first optimized for enhanced transfection efficacies as coliposomes with DOPE. All lipopolymers are better transfecting agents and highly serum compatible than commercially available PEI-25KDa. Transfection efficacies and serum compatibility of lipopolymers were found to be dependent upon the MW of PEI used for lipopolymer synthesis and percentage of cholesterol grafting on lipopolymers. Cell viability assay showed that PEI-25KDa is highly toxic as compared to all the lipopolymers. Lipopolyplexes were characterized by transmission electron microscopy, which showed the presence of spherical aggregates.
authors.affiliation: Department of Organic Chemistry, Indian Institute of Science, Bangalore 560 012, India.
authors.forename: Avinash
authors.initials: A
authors.lastname: Bajaj
authors.forename: Paturu
authors.initials: P
authors.lastname: Kondaiah
authors.forename: Santanu
authors.initials: S
authors.lastname: Bhattacharya
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002784:Cholesterol / Q000737:chemistry*; D004247:DNA / Q000378:metabolism; D004987:Ethers / Q000737:chemistry; D006367:HeLa Cells; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008970:Molecular Weight; D010714:Phosphatidylethanolamines / Q000378:metabolism; D011094:Polyethyleneimine / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis* / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D014162:Transfection / Q000379:methods*
chemical_list: C410262:1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; D004987:Ethers; D010714:Phosphatidylethanolamines; D011108:Polymers; D011094:Polyethyleneimine; D004247:DNA; D002784:Cholesterol