Physical map of bacteriophage phi29 DNA.
Restriction endonuclease EcoRI cleaves linear phi29 DNA at four points yielding five fragments (A-E) of molecular weights 6.0 x 10(6), 3.8 x 10(6), 1.3 x 10(6), 0.6 x 10(6), and 0.3 x 10(6). The relative order of the fragments was shown to be A, B, E, D, C by analysis of the EcoRI partial digestion products and from the study of the fragments obtained by treatment of protein-containing DNA with EcoRI and trypsin. The EcoRI cleavage map of phi29 DNA has been ordered relative to the genetic map by marker rescue experiments.
Inciarte
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MR
Centro de Biologia Molecular (C.S.I.C.-U.A.M.), Velázquez 144, Madrid-6, Spain.
Lázaro
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J
Salas
M
M
Viñuela
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