title: Rapid kinetic analysis of transcription elongation by Escherichia coli RNA polymerase.
abstract: Nucleotide incorporation during transcription by RNA polymerase is accompanied by pyrophosphate formation. Rapid release of pyrophosphate from the elongation complex at a rate consistent with productive transcription elongation occurs only in the presence of the correct next nucleotide for incorporation into the transcript.
authors.affiliation: Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA. johnsonro@ecu.edu
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Johnson
authors.forename: Mark
authors.initials: M
authors.lastname: Strausbauch
authors.forename: Ryan
authors.initials: R
authors.lastname: Cooper
authors.forename: J Kristen
authors.initials: JK
authors.lastname: Register
mesh_terms: D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics; D013997:Time Factors; D014158:Transcription, Genetic
chemical_list: D011756:Diphosphates; D009711:Nucleotides; D012321:DNA-Directed RNA Polymerases