title: Delivery of immunotherapy with peptide-pulsed blood in macaques.
abstract: Simple and effective delivery methods for cellular immunotherapies are needed. We assessed ex vivo pulsing of overlapping SIV Gag 15mer peptides onto either whole blood or PBMC in 15 randomly assigned SIV-infected macaques. Both delivery methods were safe and immunogenic, stimulating high levels of broad and polyfunctional Gag-specific CD4 and CD8 T cells. Delivery of overlapping Gag peptides via either whole blood or PBMC is suitable for clinical evaluation.
authors.affiliation: Department of Microbiology and Immunology, University of Melbourne, 3010, Australia.
authors.forename: Robert
authors.initials: R
authors.lastname: De Rose
authors.forename: Caroline S
authors.initials: CS
authors.lastname: Fernandez
authors.forename: Liyen
authors.initials: L
authors.lastname: Loh
authors.forename: Viv
authors.initials: V
authors.lastname: Peut
authors.forename: Rosemarie D
authors.initials: RD
authors.lastname: Mason
authors.forename: Sheilajen
authors.initials: S
authors.lastname: Alcantara
authors.forename: Jeanette
authors.initials: J
authors.lastname: Reece
authors.forename: Stephen J
authors.initials: SJ
authors.lastname: Kent
mesh_terms: D000818:Animals; D001769:Blood / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D015683:Gene Products, gag / Q000627:therapeutic use; D007167:Immunotherapy / Q000379:methods*; D007371:Interferon-gamma / Q000378:metabolism; D007963:Leukocytes, Mononuclear / Q000276:immunology*; D008254:Macaca nemestrina; D009842:Oligopeptides / Q000627:therapeutic use*; D011897:Random Allocation; D016097:Simian Acquired Immunodeficiency Syndrome / Q000276:immunology / Q000628:therapy*
chemical_list: C066594:Gag protein p27, Simian immunodeficiency virus; D015683:Gene Products, gag; D009842:Oligopeptides; D007371:Interferon-gamma