Isolation, analysis and marker utility of novel miniature inverted repeat transposable elements from the barley genome.
Four previously undescribed families of miniature inverted repeat transposable elements (MITEs) were isolated by searching barley genomic DNA using structure-based criteria. Putative MITEs were confirmed by PCR to determine their insertional polymorphism in a panel of diverse barley germplasm. Copy numbers for all these familes are somewhat low (less than 1,000 copies per family per haploid genome). In contrast to previous studies, a higher proportion of insertions of the new MITEs are found within known transposable elements (27%) than are associated with genes (15%). Preliminary studies were conducted on two of the new MITE families to test their utility as molecular markers. Insertional polymorphism levels for both the families are high and diversity trees produced by both the families are similar and congruent with known relationships among the germplasm studied, suggesting that both the MITE families are useful markers of barley genetic diversity.
Lyons
Maura
M
Division of Plant Sciences, University of Dundee at SCRI, Invergowrie, Dundee, DD2 5DA, UK.
Cardle
Linda
L
Rostoks
Nils
N
Waugh
Robbie
R
Flavell
Andrew J
AJ
D001483:Base Sequence; D004251:DNA Transposable Elements / Q000235:genetics*; D018744:DNA, Plant / Q000235:genetics*; D005819:Genetic Markers; D018745:Genome, Plant / Q000502:physiology*; D006238:Haploidy; D001467:Hordeum / Q000235:genetics*; D055029:Inverted Repeat Sequences / Q000235:genetics*; D008969:Molecular Sequence Data; D011110:Polymorphism, Genetic; D017422:Sequence Analysis, DNA
D004251:DNA Transposable Elements; D018744:DNA, Plant; D005819:Genetic Markers