Analysis of yohimbine alkaloid from Pausinystalia yohimbe by non-aqueous capillary electrophoresis and gas chromatography-mass spectrometry.
In the present work, the qualitative and quantitative analysis of Pausinystalia yohimbe-type alkaloids in the barks of Rubiaceae species is presented using different analytical approaches. Extracts of P. yohimbe were first examined by GC-MS and the major alkaloids were identified. The quantitation of yohimbine was then accomplished by non-aqueous CE (NACE) with diode array detection. This approach was selected in order to use a running buffer fully compatible with samples in organic solvent. In particular, a mixture of methanol containing ammonium acetate (20 mM) and glacial acetic acid was used as a BGE. The same analytical sample was subjected to GC-MS and NACE analysis; the different selectivity displayed by these techniques allowed different separation profiles that can be useful in phytochemical characterization of the extracts. The linear calibration ranges were all 10-1000 microg/mL for yohimbine by GC-MS and NACE analysis. The recovery of yohimbine was 91.2-94.0% with RSD 1.4-4.3%. The LOD for yohimbine were 0.6 microg/mL by GC-MS and 1.0 microg/mL by NACE, respectively. The GC-MS and NACE methods were successfully validated and applied to the quantitation of yohimbine.
Chen
Qinhua
Q
Affiliated Dongfeng General Hospital, Yunyang Medical College, Hubei, PR China. cqh77@163.com
Li
Peng
P
Zhang
Zhuo
Z
Li
Kaijun
K
Liu
Jia
J
Li
Qiang
Q
D000085:Acetates; D019342:Acetic Acid; D000470:Alkaloids / Q000032:analysis; D019075:Electrophoresis, Capillary / Q000379:methods*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D000432:Methanol; D032068:Pausinystalia / Q000737:chemistry*; D015203:Reproducibility of Results; D012997:Solvents; D015016:Yohimbine / Q000032:analysis*
D000085:Acetates; D000470:Alkaloids; D012997:Solvents; D015016:Yohimbine; D019342:Acetic Acid; C018824:ammonium acetate; D000432:Methanol