Anti-inflammatory effects of schisandrin isolated from the fruit of Schisandra chinensis Baill.
Schisandrin is the main active ingredient isolated from the fruit of Schisandra chinensis Baill. Recent studies have demonstrated that schisandrin exhibits anti-oxidative effects in vivo. In the present study, the effect of schisandrin on plasma nitrite concentration in lipopolysaccharide (LPS)-treated mice was evaluated. It also significantly inhibited carrageenan-induced paw edema and acetic acid-induced vascular permeability in mice. Furthermore, schisandrin had a protective effect on lipopolysaccharide (LPS)-induced sepsis. In vitro, our results are the first that show that the anti-inflammatory properties of schisandrin result from the inhibition of nitric oxide (NO) production, prostaglandin E(2) (PGE(2)) release, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, which in turn results from the inhibition of nuclear factor-kappaB (NF-kappaB), c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK) activities in a RAW 264.7 macrophage cell line.
Guo
Lian Yu
LY
Natural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 151-747, South Korea.
Hung
Tran Manh
TM
Bae
Ki Hwan
KH
Shin
Eun Myoung
EM
Zhou
Hong Yu
HY
Hong
Yoo Na
YN
Kang
Sam Sik
SS
Kim
Hyun Pyo
HP
Kim
Yeong Shik
YS
D000818:Animals; D000893:Anti-Inflammatory Agents / Q000302:isolation & purification / Q000494:pharmacology*; D002199:Capillary Permeability / Q000187:drug effects; D002460:Cell Line; D034242:Cyclooctanes / Q000302:isolation & purification / Q000494:pharmacology*; D051546:Cyclooxygenase 2 / Q000187:drug effects / Q000378:metabolism; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000378:metabolism; D005638:Fruit; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000188:drug therapy* / Q000209:etiology / Q000503:physiopathology; D017705:Lignans / Q000302:isolation & purification / Q000494:pharmacology*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000187:drug effects / Q000378:metabolism; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D052247:Nitric Oxide Synthase Type II / Q000187:drug effects / Q000378:metabolism; D009573:Nitrites / Q000097:blood; D011083:Polycyclic Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D032141:Schisandra / Q000737:chemistry*; D018805:Sepsis / Q000517:prevention & control
D000893:Anti-Inflammatory Agents; D034242:Cyclooctanes; D017705:Lignans; D008070:Lipopolysaccharides; D009573:Nitrites; D011083:Polycyclic Compounds; D009569:Nitric Oxide; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; C011105:schizandrin; D015232:Dinoprostone