Measurement of the humoral immune response following an incident human papillomavirus type 16 or 18 infection in young women by a pseudovirion-based neutralizing antibody assay.
We have evaluated a neutralizing antibody assay which uses human papillomavirus (HPV) type 16 (HPV-16) and HPV-18 pseudovirions carrying a secretory alkaline phosphatase reporter gene and which can potentially measure functionally relevant HPV type-specific neutralizing antibodies. The reproducibility of the assay was excellent; for HPV-16, the intra- and interassay kappa values were 0.95 and 0.90, respectively; and for HPV-18, the corresponding values were 0.90 and 0.90. This assay was used to describe the kinetics of the neutralizing antibody response in a cohort of 42 young women who were recruited soon after first intercourse and who first tested positive for HPV-16 DNA or HPV-18 DNA, or both, during follow-up. Most women seroconverted following the first detection of type-specific HPV DNA and remained seropositive until the end of follow-up. Our findings are broadly consistent with those of two other cohort studies which have measured the serological response following an incident infection by using the technically simpler virus-like-particle-based enzyme-linked immunosorbent assay.
Steele
Jane
J
CRUK Institute for Cancer Studies, University of Birmingham, Vincent Drive, Edgbaston, Birmingham B15 2TT, United Kingdom. j.c.steele@bham.ac.uk
Collins
Stuart
S
Wen
Kaisheng
K
Ryan
Gordon
G
Constandinou-Williams
Christothea
C
Woodman
Ciaran B J
CB
D000293:Adolescent; D000328:Adult; D000914:Antibodies, Viral / Q000097:blood*; D015331:Cohort Studies; D005260:Female; D052162:Human papillomavirus 16 / Q000276:immunology / Q000302:isolation & purification*; D052161:Human papillomavirus 18 / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D009500:Neutralization Tests; D030361:Papillomavirus Infections / Q000175:diagnosis* / Q000821:virology; D015203:Reproducibility of Results; D022701:Virosomes
D000914:Antibodies, Viral; D022701:Virosomes