title: In vitro uptake of β-carotene by human exfoliated colonic epithelial cells.
abstract: The possible use of isolated exfoliated colonic epithelial cells in culture as an in vitro model to study the uptake of vitamin A and β-carotene was tested. Freshly isolated exfoliated cells maintained in culture take up β-carotene in a concentration-dependent manner. The uptake was found to increase in the presence of primary bile acids such as deoxy cholate, lipids such as triolein and lecithin in a concentration-dependent manner. These results suggest that exfoliated colonic epithelial cells isolated from stool samples of human subjects can be maintained in culture and serve as a useful non-invasive system to study the bioavailability of vitamin A and provitamin A carotenoids.
authors.affiliation: Department of Biochemistry, University of Kerala, Thiruvananthapuram, India.
authors.forename: T
authors.initials: T
authors.lastname: Gireesh
authors.forename: P R
authors.initials: PR
authors.lastname: Sudhakaran
mesh_terms: D001647:Bile Acids and Salts / Q000378:metabolism; D001682:Biological Availability; D002478:Cells, Cultured / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D004847:Epithelial Cells / Q000378:metabolism*; D005243:Feces; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism*; D054709:Lecithins / Q000378:metabolism; D008954:Models, Biological; D014304:Triolein / Q000378:metabolism; D014801:Vitamin A / Q000378:metabolism*; D019207:beta Carotene / Q000378:metabolism*
chemical_list: D001647:Bile Acids and Salts; D054709:Lecithins; D019207:beta Carotene; D014801:Vitamin A; D014304:Triolein