Oral immunization with recombinant lactobacillus plantarum induces a protective immune response in mice with Lyme disease.
Mucosal immunization is advantageous over other routes of antigen delivery because it can induce both mucosal and systemic immune responses. Our goal was to develop a mucosal delivery vehicle based on bacteria generally regarded as safe, such as Lactobacillus spp. In this study, we used the Lyme disease mouse model as a proof of concept. We demonstrate that an oral vaccine based on live recombinant Lactobacillus plantarum protects mice from tick-transmitted Borrelia burgdorferi infection. Our method of expressing vaccine antigens in L. plantarum induces both systemic and mucosal immunity after oral administration. This platform technology can be applied to design oral vaccine delivery vehicles against several microbial pathogens.
del Rio
Beatriz
B
Department of Microbiology and Immunology, New York Medical College, Valhalla, New York,USA.
Dattwyler
Raymond J
RJ
Aroso
Miguel
M
Neves
Vera
V
Meirelles
Luciana
L
Seegers
Jos F M L
JF
Gomes-Solecki
Maria
M
D000284:Administration, Oral; D000818:Animals; D000907:Antibodies, Bacterial / Q000032:analysis / Q000097:blood; D001769:Blood / Q000382:microbiology; D025065:Borrelia burgdorferi / Q000235:genetics* / Q000276:immunology; D004199:Disease Vectors; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000737:chemistry; D005260:Female; D006321:Heart / Q000382:microbiology; D007070:Immunoglobulin A / Q000032:analysis; D007074:Immunoglobulin G / Q000097:blood; D018884:Ixodes; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000276:immunology*; D008193:Lyme Disease / Q000517:prevention & control*; D022123:Lyme Disease Vaccines / Q000008:administration & dosage / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H; D001743:Urinary Bladder / Q000382:microbiology
D000907:Antibodies, Bacterial; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D022123:Lyme Disease Vaccines