Determination of adulteration in apple juice by HPLC with novel optical rotation detector.
A simple HPLC method for the determination of adulteration in apple juice was developed. The method is based on the detection of D-malate, derived from racemic malic acid, which is added as an acidulant. A variable-wavelength optical rotation detector was used to determine the enantiomeric excess (ee). Using anion-exchange chromatography with a phosphate buffer eluent and UV (210 nm) detection, the limit of detection for L-malate was 2 microg. With an injection of 13.4 microg of malate, the standard deviation of the ee calibration curve was 2.5%. Several apple juice samples were analyzed according to the proposed procedure, and the results agreed with those obtained using enzymatic kits for food analysis.
Yamamoto
Atsushi
A
College of Bioscience and Biotechnology, Chubu University, Kasaugai-shi, Aichi 487-8501, Japan. akmiy@isc.chubu.ac.jp
Kawai
Mio
M
Miwa
Toshio
T
Tsukamoto
Tomoyasu
T
Kodama
Shuji
S
Hayakawa
Kazuichi
K
D001628:Beverages / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D008293:Malates / Q000032:analysis; D027845:Malus / Q000737:chemistry*; D009904:Optical Rotation
D008293:Malates; C030298:malic acid