title: A temperature-sensitive strain of tobacco mosaic virus defective in cell-to-cell movement generates an altered viral-coded protein.
abstract: An analysis has been made of the proteins synthesized in vivo and in vitro from two closely related tomato strains of tobacco mosaic virus, one of which is defective in cell-to-cell movement at a restrictive temperature [Nishiguchi et al. (1978). J. Gen. Virol. 39, 53-61]. The coat proteins of the two strains (L, and the temperature-sensitive LS-1) are identical as revealed by two-dimensional tryptic peptide maps prepared from proteins synthesized in vivo. Peptide maps of the 30,000, 130,000, and 165,000 Mr viral RNA in vitro translational products revealed a slight difference only in the 30,000 Mr polypeptides of the two strains, implicating this polypeptide as a factor mediating cell-to-cell movement of virus in the leaf.
authors.affiliation: Department of Plant Pathology, Cornell University, Ithaca, New York 14853, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Leonard
authors.forename: M
authors.initials: M
authors.lastname: Zaitlin