title: Loss of heterozygosity of the APC gene in oral squamous cell carcinoma.
abstract: The aim of this study was to investigate loss of heterozygosity (LOH) of the APC tumor suppressor gene loci, using restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR) in 40 cases of oral squamous cell carcinoma (OSCC). Observed informativity was 72.5% for APC exon 11 and 82.5% for APC exon 15. LOH at APC exon 11 was observed in 2 (6.9%) of 29 informative cases, and no LOH was observed for APC exon 15. Our results suggest that inactivation of the APC gene plays a minor role in the carcinogenesis of OSCC.
authors.affiliation: Department of Pathology, Health Science Center, Federal University of Santa Catarina, CEP 88040-370, Florianópolis, SC, Brazil. riet@ccs.ufsc.br
authors.forename: Elena R C
authors.initials: ER
authors.lastname: Rivero
authors.forename: Martinho C R
authors.initials: MC
authors.lastname: Horta
authors.forename: Eliete N
authors.initials: EN
authors.lastname: Silva Guerra
authors.forename: Alberto R
authors.initials: AR
authors.lastname: Ferraz
authors.forename: Fábio D
authors.initials: FD
authors.lastname: Nunes
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D017491:Genes, APC; D006801:Humans; D019656:Loss of Heterozygosity; D009062:Mouth Neoplasms / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length