[Experimental study of saikosaponin-D (SSd) on lipid peroxidation of hepatic fibrosis on rat].
OBJECTIVE
To study the effect of SSd on lipid peroxidation during experimental hepatic fibrosis progression.


METHODS
The experimental models of hepatic fibrosis were induced by intraperitoneal injection of dimethylnitrosamine (DMN) on rats. SSd was administered by intraperitoneal injection for 4 weeks. Serum was analyzed for alanine and aspartate aminotransferase (ALT and AST), hyaluronic acid (HA), laminin (LN), collagen IV (IV-C), malonaldehyde (MDA) and superoxide dismutase (SOD) activities. Liver samples were measured for MDA contents and SOD activities in normal group, model group and SSd group.


RESULTS
SSd significantly decreased ALT and AST activities and lowered HA, LN and IV-C contents. It enhanced SOD activities in liver, while reduced MDA contents both in serum and liver.


CONCLUSIONS
SSd has obvious effects of protecting hepatocytes and resisting hepatic fibrosis, and the mechanism may be associated with its anti-lipid peroxidation effect.
He
Yan
Y
The China-Japan Friendship Hospital, Beijing 100029, China.
Hu
Zhi-Feng
ZF
Li
Ping
P
Xiao
Cheng
C
Chen
Yu-Wu
YW
Li
Ke-Ming
KM
Guo
Jing-Zhen
JZ
Pan
Lin
L
Xiong
Jia-Peng
JP
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D024141:Collagen Type IV / Q000097:blood; D004128:Dimethylnitrosamine / Q000009:adverse effects; D006820:Hyaluronic Acid / Q000097:blood; D007797:Laminin / Q000097:blood; D015227:Lipid Peroxidation / Q000187:drug effects*; D008103:Liver Cirrhosis / Q000097:blood / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism*; D008315:Malondialdehyde / Q000097:blood; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D051381:Rats; D012503:Saponins / Q000494:pharmacology* / Q000627:therapeutic use; D013482:Superoxide Dismutase / Q000097:blood
D024141:Collagen Type IV; D007797:Laminin; D012503:Saponins; D008315:Malondialdehyde; D009828:Oleanolic Acid; D006820:Hyaluronic Acid; D013482:Superoxide Dismutase; D001219:Aspartate Aminotransferases; D004128:Dimethylnitrosamine; C025759:saikosaponin D