Immune responsiveness of a novel peptidoglycan conjugate prepared from surface Candida immunogens: mannan and CR3-related protein.
Candida albicans expresses a CR3-related protein (CR3-RP) antigenically, structurally and functionally related to human adhesion glycoprotein, also known as Mac-l, the iC3b receptor, or complement receptor type 3. The purpose of the present study was to analyze the immunogenic properties of a novel CR3-RP glycoconjugate in a rabbit model. Cell-mediated responses revealed immunoenhancement triggered by CR3-RP glycoconjugate with respect to expression of IL-2 receptor subunit CD25 on B-lymphocytes and inductive increase of the CD4(+)/CD8(+) ratio compared with unconjugated cell wall mannan (P<0.001). Active immunization with the CR3-RP glycoconjugate resulted in an effective IgM-IgG isotypic switch even after the second booster. Altogether, it could be assumed that the novel peptide glycoconjugate is a prospective antigenic candidate for further Candida vaccine design.
Paulovicová
Ema
E
Center for Glycomics, Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia.
Bystrický
Slavomír
S
Machová
Eva
E
Bujdáková
Helena
H
D000818:Animals; D000908:Antibodies, Fungal / Q000097:blood; D000946:Antigens, Fungal / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology; D016516:CD4-CD8 Ratio; D002176:Candida albicans / Q000276:immunology*; D015815:Cell Adhesion Molecules / Q000276:immunology*; D005260:Female; D005656:Fungal Proteins / Q000276:immunology*; D005657:Fungal Vaccines / Q000276:immunology*; D007117:Immunization, Secondary; D007074:Immunoglobulin G / Q000097:blood; D007075:Immunoglobulin M / Q000097:blood; D053645:Interleukin-2 Receptor alpha Subunit / Q000096:biosynthesis; D008351:Mannans / Q000276:immunology*; D010457:Peptidoglycan / Q000276:immunology*; D011817:Rabbits
D000908:Antibodies, Fungal; D000946:Antigens, Fungal; D015815:Cell Adhesion Molecules; D005656:Fungal Proteins; D005657:Fungal Vaccines; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D053645:Interleukin-2 Receptor alpha Subunit; D008351:Mannans; D010457:Peptidoglycan; C097687:alphaINT1 protein, Candida albicans