title: Pharmacogenetics of hormone replacement therapy for climacteric symptoms.
abstract: Hormone replacement therapy (HRT) is highly effective for women suffering from climacteric symptoms, with occasionally severe side effects. To determine which women needs HRT for climacteric symptoms indeed, pharmacogenetical approach for HRT was performed. Under the condition of minimal HRT, 33 patients required HRT for more than 1 year and the remaining 156 did not. Three single nucleotide polymorphisms (SNPs) in estrogen receptor alpha (ERalpha) gene and 3 SNPs and a microsatellite polymorphism in estrogen receptor beta (ERbeta) gene were analyzed using LightTyper and PCR. Homozygous for 18 CA repeats of D14S1026 (OR 8.00, 95% CI 2.56-25.02, P<0.001) and rs1256049 (OR 6.35, 95% CI 2.38-16.92, P=0.004) in ERbeta associated with minimal HRT. In contrast, rs1271572 in 789bp upstream region of ERbeta (OR 0.30, 95% CI 0.14-0.65, P=0.002) gene decreased HRT. rs2228480 in ERalpha gene also increased HRT. Tailored decisions can be expected on the future use of HRT referring genetic polymorphisms of individuals.
authors.affiliation: Department of Molecular Epidemiology, Medical Research Institute, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
authors.forename: Chikari
authors.initials: C
authors.lastname: Takeo
authors.forename: Kanako
authors.initials: K
authors.lastname: Ugai
authors.forename: Jungo
authors.initials: J
authors.lastname: Araki
authors.forename: Ling
authors.initials: L
authors.lastname: Zhang
authors.forename: Masanori
authors.initials: M
authors.lastname: Baba
authors.forename: Wataru
authors.initials: W
authors.lastname: Ohashi
authors.forename: Koichi
authors.initials: K
authors.lastname: Ueno
authors.forename: Yoichi
authors.initials: Y
authors.lastname: Suzuki
authors.forename: Keiko
authors.initials: K
authors.lastname: Amano
authors.forename: Aizan
authors.initials: A
authors.lastname: Hirai
authors.forename: Masaaki
authors.initials: M
authors.lastname: Muramatsu
mesh_terms: D004958:Estradiol / Q000008:administration & dosage*; D047628:Estrogen Receptor alpha / Q000819:agonists / Q000235:genetics*; D047629:Estrogen Receptor beta / Q000819:agonists / Q000235:genetics*; D015914:Estrogen Replacement Therapy; D005260:Female; D006720:Homozygote; D006801:Humans; D007564:Japan; D008593:Menopause / Q000187:drug effects / Q000235:genetics*; D018895:Microsatellite Repeats; D010597:Pharmacogenetics; D020641:Polymorphism, Single Nucleotide
chemical_list: D047628:Estrogen Receptor alpha; D047629:Estrogen Receptor beta; D004958:Estradiol