The emerging role of MS in structure elucidation of protein-nucleic acid complexes.
Developments in MS enable us to apply this technique to non-covalent complexes, defining their stoichiometry, subunit interactions and architectural organization. We illustrate the application of this non-covalent MS approach to uncovering the overall topological arrangements of subunits and interactions within RNA-protein complexes studied in our laboratory over the last 5 years. These studies exemplify the emerging role and potential of MS as a complementary structural biology methodology and demonstrate its unique niche in investigations of dynamic or heterogeneous protein-nucleic acid complexes, which are not accessible to classical high-resolution structural biology techniques.
Gordiyenko
Yuliya
Y
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge, UK.
Robinson
Carol V
CV
D015870:Gene Expression / Q000235:genetics; D006801:Humans; D013058:Mass Spectrometry; D009696:Nucleic Acids / Q000737:chemistry* / Q000378:metabolism*; D011485:Protein Binding; D011506:Proteins / Q000737:chemistry* / Q000378:metabolism*
D009696:Nucleic Acids; D011506:Proteins