title: A transformation procedure for recalcitrant tomato by addressing transgenic plant-recovery limiting factors.
abstract: Agrobacterium tumefaciens technology is the battle horse for tomato genetic transformation. However, tomato varieties with low regeneration capacity are very difficult to transform. In the past, tomato transformation through Agrobacterium infection was focused on varieties capable of high regeneration yield, while successful transformation of low regenerable cultivars has not been reported. The genotype response to tissue culture conditions is believed to drive the frequency of regeneration of transgenic plant, whereas the capacity for cell proliferation could determine the transformation efficiency through this technology. The Campbell-28 cultivar is an example of constraints arising from a high morphogenetic potential with low conversion compared to normal plants. In the present work the roles that contribute to improved transgenic plant recovery from this recalcitrant variety were explored for factors like Agrobacterium concentration and antibiotics for bacterial removal and transformant selection. Analysis of the efficiency from independent transformation experiments revealed a more than twofold increase of transformant regeneration after selection on ammonium glufosinate compared to kanamycin selection, showing a transformation efficiency of 21.5%.
authors.affiliation: Plant Department, Center for Genetic Engineering and Biotechnology, Havana, Cuba. alejandro.fuentes@cigb.edu.cu
authors.forename: Alejandro D
authors.initials: AD
authors.lastname: Fuentes
authors.forename: Pedro L
authors.initials: PL
authors.lastname: Ramos
authors.forename: Yadira
authors.initials: Y
authors.lastname: Sánchez
authors.forename: Danay
authors.initials: D
authors.lastname: Callard
authors.forename: Aleines
authors.initials: A
authors.lastname: Ferreira
authors.forename: Kenia
authors.initials: K
authors.lastname: Tiel
authors.forename: Karen
authors.initials: K
authors.lastname: Cobas
authors.forename: Raisa
authors.initials: R
authors.lastname: Rodríguez
authors.forename: Carlos
authors.initials: C
authors.lastname: Borroto
authors.forename: Vivian
authors.initials: V
authors.lastname: Doreste
authors.forename: Merardo
authors.initials: M
authors.lastname: Pujol
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D005822:Genetic Vectors / Q000235:genetics*; D018551:Solanum lycopersicum / Q000235:genetics* / Q000382:microbiology*; D030821:Plants, Genetically Modified / Q000502:physiology*; D014162:Transfection / Q000379:methods*; D014170:Transformation, Genetic / Q000235:genetics*