Single base interrogation by a fluorescent nucleotide: each of the four DNA bases identified by fluorescence spectroscopy.
Nucleoside C, which contains a rigid nitroxide spin label, is effectively reduced in DNA by sodium sulfide to the corresponding amine, yielding a fluorescent probe (Cf) that can report the identity of its base-pairing partner in duplex DNA.
Cekan
Pavol
P
University of Iceland, Science Institute, Dunhaga 3, 107 Reykjavik, Iceland.
Sigurdsson
Snorri Th
ST
D000225:Adenine / Q000032:analysis; D002851:Chromatography, High Pressure Liquid; D003596:Cytosine / Q000032:analysis; D004247:DNA / Q000032:analysis*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006147:Guanine / Q000032:analysis; D009705:Nucleosides / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013941:Thymine / Q000032:analysis
D005456:Fluorescent Dyes; D009705:Nucleosides; D006147:Guanine; D003596:Cytosine; D004247:DNA; D000225:Adenine; D013941:Thymine