Plasticity of the Hsp90 chaperone machine in divergent eukaryotic organisms.
Hsp90 is critical for the regulation and activation of numerous client proteins critical for diverse functions such as cell growth, differentiation, and reproduction. Cytosolic Hsp90 function is dependent on a battery of co-chaperone proteins that regulate the ATPase activity of Hsp90 function or direct Hsp90 to interact with specific client proteins. Little is known about how Hsp90 complexes vary between different organisms and how this affects the scope of clients that are activated by Hsp90. This study determined whether ten distinct Hsp90 co-chaperones were encoded by genes in 19 disparate eukaryotic organisms. Surprisingly, none of the co-chaperones were present in all organisms. The co-chaperone Hop/Sti1 was most widely dispersed (18 out of 19 species), while orthologs of Cdc37, which is critical for the stability and activation of diverse protein kinases in yeast and mammals, were identified in only nine out of 19 species examined. The organism with the smallest proteome, Encephalitozoon cuniculi, contained only three of these co-chaperones, suggesting a correlation between client diversity and the complexity of the Hsp90 co-chaperone machine. Our results suggest co-chaperones are critical for cytosolic Hsp90 function in vivo, but that the composition of Hsp90 complexes varies depending on the specialized protein folding requirements of divergent species.
Johnson
Jill L
JL
Department of Microbiology, Molecular Biology and Biochemistry and the Center for Reproductive Biology, Moscow, ID 83844-3052, USA. jilljohn@uidaho.edu
Brown
Celeste
C
D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D005057:Eukaryotic Cells / Q000378:metabolism*; D014644:Genetic Variation; D016678:Genome / Q000235:genetics; D018841:HSP90 Heat-Shock Proteins / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D008969:Molecular Sequence Data; D010271:Parasites / Q000235:genetics; D010802:Phylogeny; D020543:Proteome / Q000378:metabolism; D017386:Sequence Homology, Amino Acid
D018841:HSP90 Heat-Shock Proteins; D020543:Proteome