title: Preparative chromatographic resolution of racemates using HPLC and SFC in a pharmaceutical discovery environment.
abstract: The preparative chromatographic resolution of racemates has become a standard approach for the generation of enantiomers in pharmaceutical discovery laboratories. This paper will discuss the use of preparative HPLC and SFC to generate individual enantiomers for discovery activities. Analytical HPLC and SFC method development to rapidly screen chiral stationary phases and solvent combinations will be presented. The usefulness of preparative chromatographic resolution of racemates will be demonstrated through the presentation of numerous non-routine case studies from the laboratories at Amgen.
authors.affiliation: Amgen, One Kendall Square, Bldg 1000, Cambridge, MA 02139, USA. millerl@amgen.com
authors.forename: Larry
authors.initials: L
authors.lastname: Miller
authors.forename: Matt
authors.initials: M
authors.lastname: Potter
mesh_terms: D000688:Amylose / Q000031:analogs & derivatives / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D055808:Drug Discovery; D013058:Mass Spectrometry; D004364:Pharmaceutical Preparations / Q000302:isolation & purification*; D048448:Phenylcarbamates / Q000737:chemistry; D013237:Stereoisomerism
chemical_list: D004364:Pharmaceutical Preparations; D048448:Phenylcarbamates; C080033:Chiralpak AD; D000688:Amylose