Padlock and proximity probes for in situ and array-based analyses: tools for the post-genomic era.
Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize biological systems in health and disease. We discuss the general problem of detection specificity and present the approach our group has taken, involving the reformatting of analogue biological information to digital reporter segments of genetic information via a series of DNA ligation assays. The assays enable extensive, coordinated analyses of the numbers and locations of genes, transcripts and protein.
Landegren
Ulf
U
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Sweden. ulf.landegren@genpat.uu.se
Dahl
Fredrik
F
Nilsson
Mats
M
Fredriksson
Simon
S
Banér
Johan
J
Gullberg
Mats
M
Jarvius
Jonas
J
Gustafsdottir
Sigrun
S
Söderberg
Ola
O
Ericsson
Olle
O
Stenberg
Johan
J
Schallmeiner
Edith
E