Phytotoxic effects of cyanobacteria extract on Lemna minor and Myriophyllum spicatum phyto-tolerance and superoxide dismutase activity.
The research on the effects of microcystins on aquatic plants has increased. Some aquatic plants have some tolerance to microcystins but the mechanism of the tolerance is still unknown. In this experiment, we used microcystins of different concentrations to study the toxic effect in Lemna minor and Myriophyllum spicatum. Experiments were carried out with a range of microcysitns levels (equivalent to 0, 0.1, 0.5, 1.0, and 4.3 mg/L). The growth of L. minor (as fresh weight) and chlorophyll a content were significantly reduced and superoxide dismutase (SOD) activity was significantly decreased at microcystins concentration up to 0.5 mg/L. The growth of M. spicatum was affected, only weakly, by microcystins and 0.5 mg/L and these treatments caused significant decrease in chlorophyll a content. Besides, the SOD activity of M. spicatum positively correlated to microcystins concentration (P < 0.01). The result indicated that M. spicatum was more tolerant to microcystins than L. minor and the induced SOD activity may contribute to the tolerance. The experiment also indicated that catalase (CAT) activity was not significantly influenced by microcystin for both the two tested aquatic plants.
Yi
Ding
D
Hubei Key Laboratory of Urban Environmental Ecology, Central China Normal University, Wuhan 430079, China.
Yijun
Zhao
Z
Xue
Bai
B
Zhihui
Fang
F
Kai
Cheng
C
D029064:Araceae / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D002374:Catalase / Q000378:metabolism; D002734:Chlorophyll / Q000032:analysis; D000077194:Chlorophyll A; D019684:Magnoliopsida / Q000187:drug effects* / Q000201:enzymology / Q000254:growth & development; D052998:Microcystins / Q000633:toxicity*; D013482:Superoxide Dismutase / Q000378:metabolism*
D052998:Microcystins; D002734:Chlorophyll; D002374:Catalase; D013482:Superoxide Dismutase; D000077194:Chlorophyll A