title: [FK228 promotes the IL-3 mediated proliferation and differentiation of human erythroid progenitor cells].
abstract: OBJECTIVE
  To investigate the role of histone deacetylases (HDACs) inhibitor FK228 in the IL-3 mediated proliferation and differentiation of human erythroid progenitor cells.
  
  
  METHODS
  CD34(+) cells were separated from the granulocyte colony-stimulating factor(G-CSF)-mobilized peripheral blood of cancer patients and cultured for 7 days with FK228 of different concentrations, stem cell factor(SCF) and interleukin 3 (IL-3) in serum-free medium. Flow cytometry was performed to analyze the expression of CD14, GPA, CD15 and CD36 on the cells and the colony assay of erythroid cells was conducted. Then the CD36(+)GPA(-) cells were enriched and cultured with with IL-3 and 0.5 microg/L FK228 supplemented serum-free semisolid cultures and the formation test of erythroid colony was conducted.
  
  
  RESULTS
  FK228 enhanced the generation of CD36(+) cells in a dose-dependent manner, in spite of that it displayed no influence on the generation of CD14(+) and CD15(+) cells. 0.5 g/L FK228 induced a remarkable increase in the cell number of CD36(+) cells cultured with IL-3; FK228 also significantly increased the number and size of CD36(+) cells colony cultured with IL-3 containing semisolid cultures. Moreover, FK228 augmented the formation of CD36(+) cells colony from CD36(+)GPA(-) cells cultured with IL-3.
  
  
  CONCLUSIONS
  FK228 can promote the IL-3 mediated proliferation and differentiation of human erythroid progenitor cells.
authors.affiliation: Department of Oncology, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Zhao-Cai
authors.initials: ZC
authors.lastname: Yu
authors.forename: Wen-Chao
authors.initials: WC
authors.lastname: Liu
authors.forename: Du-Hu
authors.initials: DH
authors.lastname: Liu
authors.forename: Ying
authors.initials: Y
authors.lastname: Huang
authors.forename: Xiao-Ming
authors.initials: XM
authors.lastname: Shi
authors.forename: Xiang-Hui
authors.initials: XH
authors.lastname: You
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D018955:CD36 Antigens / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D047630:Depsipeptides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D016256:Lewis X Antigen / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism
chemical_list: D018955:CD36 Antigens; D047630:Depsipeptides; D056572:Histone Deacetylase Inhibitors; D007377:Interleukin-3; D016256:Lewis X Antigen; D018950:Lipopolysaccharide Receptors; C087123:romidepsin