FISH for mapping single copy genes.
During the past two decades fluorescent in-situ hybridization (FISH) has become a standard technique to directly localize, orient, and order genes in the genomes of a wide range of species. Despite the availability of a variety of probes, probe labeling and signal-detection systems, and advanced image analysis software, the core procedures used to carry out FISH remain the same. A detailed overview of these procedures, including target preparation (metaphase/interphase chromosomes and DNA fibers), probe labeling, in-situ hybridization, signal detection, and imaging, is here provided in a stepwise manner.
Raudsepp
Terje
T
Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX, USA.
Chowdhary
Bhanu P
BP
D002478:Cells, Cultured; D002877:Chromosomes, Human / Q000378:metabolism; D004247:DNA / Q000378:metabolism; D015342:DNA Probes / Q000378:metabolism; D018628:Gene Dosage / Q000235:genetics*; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D007399:Interphase; D008677:Metaphase; D020161:Physical Chromosome Mapping
D015342:DNA Probes; D004247:DNA