title: [Immune response induced by Rpf domain from Micrococcus luteus in mice].
abstract: OBJECTIVE
  To investigate the immunobiology of Rpf domain from Micrococcus luteus.
  
  
  METHODS
  BALB/c mice were immunized with Rpf domain three times at 2-week interval. ELISA was used to detect the title of the anti-Rpf domain antibody titer in the immunized mice sera. The spleen lymphocytes of the immunized mice were separated and the stimulation index (SI) was measured by MTT colorimetry. The levels of secreted IFN-gamma, IL-10 and IL-12 upon specific antigen stimulation was detected by ELISA. The Rpf domain immunized BALB/c mice were intravenously infected with 10(5) CFU MTB H37Rv. The number of CFU in the spleens was determined four weeks after final injection.
  
  
  RESULTS
  The titer of the specific antibody in sera of the immunized BALB/c mice was 1:128 000. The SI of Rpf domain immunized group (2.10+/-0.12) was significantly higher than that of saline immunized group (0.90+/-0.21). The lever of IFN-gamma, IL-10 and IL-12 levels in culture supernatant of spleen lymphocytes from the fusion protein immunized mice was (1 126+/-36) ng/L, (368+/-13) ng/L and (289+/-14) ng/L, respectively, which was markedly higher than that of saline immunized group (P<0.01). Compared with normal saline immunized mice (6.64+/-0.13) four weeks after final injection, dramatic reduction in MTB replication was observed in the spleen (5.03+/-0.11) from the BALB/c mice immunized with fusion proteins.
  
  
  CONCLUSIONS
  Rpf domain can be used as a candidate for a new TB vaccine.
authors.affiliation: Department of Clinical Laboratory, Fourth Military Medical University, Xi'an 710032, China. fanailin@fmmu.edu.cn
authors.forename: Ai-Lin
authors.initials: AL
authors.lastname: Fan
authors.forename: Wen
authors.initials: W
authors.lastname: Jian
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Shi
authors.forename: Ming-Quan
authors.initials: MQ
authors.lastname: Su
authors.forename: Yin-Lan
authors.initials: YL
authors.lastname: Bai
authors.forename: Jing
authors.initials: J
authors.lastname: Ma
authors.forename: Zhi-Kai
authors.initials: ZK
authors.lastname: Xu
authors.forename: Xiao-Ke
authors.initials: XK
authors.lastname: Hao
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D016207:Cytokines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D018664:Interleukin-12 / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D016982:Micrococcus luteus / Q000378:metabolism*; D009169:Mycobacterium tuberculosis / Q000276:immunology / Q000472:pathogenicity; D011897:Random Allocation; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013154:Spleen / Q000166:cytology / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D016207:Cytokines; D011993:Recombinant Fusion Proteins; C113704:resuscitation-promoting factor, bacteria; D016753:Interleukin-10; D018664:Interleukin-12; D007371:Interferon-gamma