Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer.
The accurate assessment of nodal involvement in prostate cancer is crucial to planning treatment, yet there is a shortage of noninvasive imaging techniques capable of visualizing nodal lesions directly. This study demonstrates the feasibility of using recombinant human adenoviral vectors to detect nodal metastases in a human prostate cancer model. This was achieved by the prostate-restricted expression of optical and positron emission tomography (PET) imaging reporter genes by the viral vector coupled with the innate lymphotropic properties of adenovirus. We show that peritumoral administration of these vectors results in the direct detection of reporter gene expression in metastatic lesions within sentinel lymph nodes. Notably, this approach parallels the current lymphoscintigraphy method but enables the direct PET visualization of sentinel lymph node metastases, eliminating the need for invasive lymphadenectomy. These findings may lead to more effective diagnostic and therapeutic strategies for individuals with advanced-stage prostate cancer.
Burton
Jeremy B
JB
Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging and Jonsson Comprehensive Cancer Center, David Geffen School of Medicine, University of California-Los Angeles, Los Angeles, California 90095-1735, USA.
Johnson
Mai
M
Sato
Makoto
M
Koh
Sok Boon S
SB
Mulholland
David J
DJ
Stout
David
D
Chatziioannou
Arion F
AF
Phelps
Michael E
ME
Wu
Hong
H
Wu
Lily
L
D000256:Adenoviridae / Q000235:genetics* / Q000378:metabolism*; D000818:Animals; D015967:Gene Expression Regulation, Viral; D006801:Humans; D007211:Indoles; D008198:Lymph Nodes / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D009362:Neoplasm Metastasis; D009368:Neoplasm Transplantation; D049268:Positron-Emission Tomography / Q000379:methods; D011471:Prostatic Neoplasms / Q000235:genetics* / Q000378:metabolism*; D014057:Tomography, X-Ray Computed / Q000379:methods
D007211:Indoles; C499748:SCIO-469