Identification and characterization of novel immunogenic proteins of Streptococcus suis serotype 2.
Streptococcus suis, a zoonotic pathogen, caused serious outbreaks in humans with high mortality rates in the past decade. To develop safer and more effective vaccines, particularly for human protection, cell wall and extracellular proteins of S. suis serotype 2 were analyzed by an immunoproteomic approach in this study. Thirty-two proteins with high immunogenicity were identified and 22 of them were newly identified. Further analyses of 9 selected proteins revealed that (1) these 9 proteins were expressed in all tested virulent S. suis serotype 2 isolates, (2) antisera against 6 of the selected proteins efficiently killed the bacteria by opsonized phagocytosis in human blood, and (3) significantly higher levels of serum antibodies against 3 proteins were detected in both patients and infected swines. Therefore, our results suggest the 3 proteins (SSU98_0197, SSU98_1094 and SSU1664) have strong potential to be vaccine candidates.
Geng
Hongran
H
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, No. 20 Dongda Street, Fengtai District, Beijing 100071, People's Republic of China.
Zhu
Li
L
Yuan
Yuan
Y
Zhang
Wei
W
Li
Wenjun
W
Wang
Jie
J
Zheng
Yuling
Y
Wei
Kaihua
K
Cao
Wuchun
W
Wang
Hengliang
H
Jiang
Yongqiang
Y
D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood; D001426:Bacterial Proteins / Q000737:chemistry*; D001483:Base Sequence; D017931:DNA Primers; D015180:Electrophoresis, Gel, Two-Dimensional; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007106:Immune Sera; D016247:Information Storage and Retrieval; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017011:Streptococcus suis / Q000737:chemistry* / Q000276:immunology; D013552:Swine
D000907:Antibodies, Bacterial; D001426:Bacterial Proteins; D017931:DNA Primers; D007106:Immune Sera