title: Biochemical and hematological reference ranges for Amazon freshwater turtle, Podocnemis expansa (Reptilia: Pelomedusidae), with morphologic assessment of blood cells.
abstract: Blood parameters are useful to measure physiological disturbances in chelonians. Thus they can provide important information for the diagnosis and prognosis of diseases. The aim of this paper was to determine the reference range of plasma glucose, total protein, triglycerides, cholesterol and urea levels, as well as hematocrit, hemoglobin, red blood cell, thrombocyte and white blood cell counts for healthy Podocnemis expansa (Schweigger, 1812), bred in a turtle farm in the Amazonas State, Brazil. Plasma glucose, hemoglobin, mean corpuscular volume (MCV), lymphocytes and heterophils were the parameters with the smallest variations. Significant (p<0.001) correlations between red blood cells count and hematocrit and hemoglobin concentration were found. Turtle P. expansa had lymphocytes, azurophils, heterophils, eosinophils and basophils with morphologic features similar to the ones of others turtles species. No investigated blood parameter was influenced by sex. In further studies, the established reference ranges might be useful for the health assessment of this turtle species.
authors.affiliation: Department of Physiological Sciences, Institute of Biological Sciences, Federal University of Amazonas, Av. Gal. Rodrigo Octávio Jordão Ramos, 3000, 69.077-000 Manaus, Amazonas State, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Oliveira-Júnior
authors.forename: M
authors.initials: M
authors.lastname: Tavares-Dias
authors.forename: J L
authors.initials: JL
authors.lastname: Marcon
mesh_terms: D000818:Animals; D001772:Blood Cell Count / Q000662:veterinary; D001786:Blood Glucose / Q000032:analysis; D001798:Blood Proteins / Q000032:analysis; D002784:Cholesterol / Q000097:blood; D003247:Conservation of Natural Resources; D005260:Female; D006400:Hematocrit / Q000662:veterinary; D006454:Hemoglobins / Q000032:analysis; D008297:Male; D012016:Reference Values; D018709:Statistics, Nonparametric; D014280:Triglycerides / Q000097:blood; D014426:Turtles / Q000097:blood*; D014508:Urea / Q000097:blood
chemical_list: D001786:Blood Glucose; D001798:Blood Proteins; D006454:Hemoglobins; D014280:Triglycerides; D014508:Urea; D002784:Cholesterol