Fine structure and morphogenesis of the sclerite epicuticle in the Atlantic shore crab Carcinus maenas.
Three basic sublayers are identified in the epicuticle of the mineralised sclerites of the Atlantic shore crab Carcinus maenas (Crustacea, Decapoda): the surface coat, the cuticulin layer, and the inner epicuticle. Their morphogenesis and subsequent changes are described throughout the moulting cycle in the normal cuticle and the cuticular structures, namely the sensory bristles and epicuticular spines. At first, the cuticulin layer begins to form just after apolysis. This layer is built directly over the plasma membrane and immediately appears as a membrane-like structure 40 nm thick, composed of five symmetrically arranged laminae: two inner electron-lucent leaflets sandwiched between two thick electron-dense leaflets and separated by a thin dense median stratum. Elaboration of the inner epicuticle below the cuticulin layer is thought to occur via an intussusceptive process involving the pore canal cell extensions as transport routes. The inner epicuticle is made of vertically oriented microfibres embedded in an electron-dense matrix material. During the second half of the premoult period, the surface coat is deposited on the upper side of the cuticulin layer.
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National Fund for the Scientific Research (FNRS, Belgium), Laboratoire de Biologie générale et de Morphologie ultrastructurale, Université de Liège, Institut de Zoologie, Liège, Belgium.