Anti-CD22-MCC-DM1 and MC-MMAF conjugates: impact of assay format on pharmacokinetic parameters determination.
CD22 represents a promising target for antibody-drug conjugate therapy in the context of B cell malignancies since it rapidly internalizes, importing specifically bound antibodies with it. To determine the pharmacokinetic parameters of anti-CD22-MCC-DM1 and MC-MMAF conjugates, various approaches to quantifying total and conjugated antibody were investigated. Although the total antibody assay formats gave similar results for both conjugates, the mouse pharmacokinetic profile for the anti-CD22-MCC-DM1 and MC-MMAF appeared significantly different depending on the conjugated antibody assay format. Since these differences significantly impacted the PK parameters determination, we investigated the effect of the drug/antibody ratio on the total and conjugated antibody quantification using multiple assay formats. Our investigations revealed the limitations of some assay formats to quantify anti-CD22-MCC-DM1 and MC-MMAF with different drug load and in the context of a heterogeneous ADC population highlight the need to carefully plan the assay strategy for the total and conjugated antibody quantification in order to accurately determine the ADC PK parameters.
Stephan
Jean-Philippe
JP
Department of Assay and Automation Technology, Genentech, Inc., 1 DNA Way, South San Francisco, California 94080, USA. stephanj@gene.com
Chan
Pamela
P
Lee
Chien
C
Nelson
Christopher
C
Elliott
James Michael
JM
Bechtel
Charity
C
Raab
Helga
H
Xie
David
D
Akutagawa
Jon
J
Baudys
Jakub
J
Saad
Ola
O
Prabhu
Saileta
S
Wong
Wai Lee T
WL
Vandlen
Richard
R
Jacobson
Fred
F
Ebens
Allen
A
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis / Q000276:immunology / Q000378:metabolism* / Q000493:pharmacokinetics*; D006801:Humans; D018796:Immunoconjugates / Q000032:analysis / Q000276:immunology / Q000378:metabolism* / Q000493:pharmacokinetics*; D008301:Maleimides / Q000378:metabolism*; D008453:Maytansine / Q000031:analogs & derivatives* / Q000378:metabolism*; D051379:Mice; D009842:Oligopeptides / Q000378:metabolism*; D012680:Sensitivity and Specificity; D051918:Sialic Acid Binding Ig-like Lectin 2 / Q000276:immunology*
D000911:Antibodies, Monoclonal; D018796:Immunoconjugates; D008301:Maleimides; D009842:Oligopeptides; D051918:Sialic Acid Binding Ig-like Lectin 2; C513576:monomethylauristatin F; D008453:Maytansine