Peroxidase and laccase as catalysts for removal of the phenylhydrazide protecting group under mild conditions.
A new method is proposed for the removal of the phenylhydrazide protecting group by the action of peroxidase or laccase, the enzymes attributed to the class of oxidoreductases. The deblocking procedure is performed under mild oxidative conditions, i.e., aqueous solution and neutral or close to neutral pH. Such mild oxidizing agents as 1 mM H(2)O(2) and air oxygen are used for unmasking. The method is available for the deblocking of both alpha- and gamma-carboxyl groups. The enzyme-catalyzed removal of the phenylhydrazide protecting group causes no oxidative modification nor destruction of methionine or tryptophan side chains.
Semenov
A N
AN
Russian-German Joint Venture Constanta, Gruzinsky per, 3/2, Moscow, Russia.
Lomonsova
I V
IV
Berezin
V I
VI
Titov
M I
MI