[Isolation of a novel fructose-1,6-bisphosphate aldolase gene from Codonopsis lanceolata and analysis of the response of this gene to abiotic stresses].
A cDNA clone containing a fructose-1,6-bisphosphate aldolase (ALD) gene, designated ClAldC, was isolated from a medicinal plant Codonopsis lanceolata. ClAldC is predicted to encode a precursor protein of 358 amino acid residues, and its sequence shares high degrees of homology with a number of other ALDs. The expression of ClAldC in different C. lanceolata organs was analyzed using reverse transcriptase (RT)-PCR. The results showed that ClAldC expressed high in stems of intact plant, while expressed at low level in leaves and roots. In addition, the expression of ClAldC under different abiotic stresses was analyzed at different time points. Three tested abiotic stimuli, anoxygenic stress, hydrogen peroxide and chilling, triggered a significant induction of ClAldC within 2-8 h post-treatment. However, there was no induction under other four stresses, NaCI, wounding, light and dark. The positive responses of ClAldC to the three abiotic stimuli suggested that C. lanceolata ClAldC may help to protect against environmental stresses such as anoxia, chilling and oxidative stress.
Purev
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M
Kim
M K
MK
Samdan
N
N
Yang
D-C
DC
D029745:Codonopsis / Q000201:enzymology* / Q000235:genetics; D018076:DNA, Complementary / Q000235:genetics; D004792:Enzyme Precursors / Q000096:biosynthesis* / Q000235:genetics; D005634:Fructose-Bisphosphate Aldolase / Q000096:biosynthesis* / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D009928:Organ Specificity; D018384:Oxidative Stress; D010935:Plant Diseases / Q000235:genetics; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics; D010940:Plant Proteins / Q000096:biosynthesis* / Q000235:genetics; D018517:Plant Roots / Q000201:enzymology / Q000235:genetics; D018547:Plant Stems / Q000201:enzymology / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid
D018076:DNA, Complementary; D004792:Enzyme Precursors; D010940:Plant Proteins; D005634:Fructose-Bisphosphate Aldolase