Characterization of spirostanol saponins in Solanum torvum by high-performance liquid chromatography/evaporative light scattering detector/electrospray ionization with multi-stage tandem mass spectrometry.
High-performance liquid chromatography with an evaporative light scattering detector and electrospray ionization multistage tandem mass spectrometry (HPLC/ELSD/ESI-MS(n)) was used to identify spirostanol saponins in a saponin extract of Solanum torvum. The fragmentation behavior of saponins was studied using ESI-MS(1-3) in positive ion mode. The MS(n) spectra of the [M+H](+) ions provide structural information including aglycone type and the nature and sequence of sugars. The use of ELSD allowed the profiling of the nonchromophore-containing saponins in this plant. The MS analysis established in this study with known saponins was successfully applied to tentatively identify two new siprostanol glycosides, neosolaspigenin 6-O-beta-D-quinovopyranoside and solagenin 6-O-[beta-D-xylopyranosyl-(1 --> 3)-O-beta-D-quinovopyranoside].
Lu
Yuanyuan
Y
Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.
Luo
Jianguang
J
Xu
Deran
D
Huang
Xuefeng
X
Kong
Lingyi
L
D002851:Chromatography, High Pressure Liquid; D010936:Plant Extracts / Q000737:chemistry; D010946:Plants, Medicinal; D012503:Saponins / Q000737:chemistry*; D012542:Scattering, Radiation; D032322:Solanum / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013150:Spirostans / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods*
D010936:Plant Extracts; D012503:Saponins; D013150:Spirostans