Genetic variants in protein kinase C zeta gene and type 2 diabetes risk: a case-control study of a Chinese Han population.
BACKGROUND
Previous investigations of the protein kinase C zeta (PRKCZ) gene, a susceptibility factor for type 2 diabetes mellitus (T2DM), have focussed on its single nucleotide polymorphisms (SNPs). In a departure from these earlier studies, we tested six tagging markers of PRKCZ based on the Hapmap database to evaluate their association with T2DM.


METHODS
PCR-RFLP was performed to test the genotypes in type 2 diabetes patients (n = 343) and normal controls (n = 429). Insulin-related indexes were calculated from levels of fasting plasma insulin (FPI) and fasting plasma glucose (FPG).


RESULTS
The variant genotypes, rs3753242TT, rs2503706CT, rs2503706CT/ TT and rs262654TT were associated with T2DM. And the significance remained for rs3753242 TT (p < 0.001) after the false discovery rate (FDR) correction. There was a doubling in the prevalence of the TTGCCC haplotype in the case subjects (14.2%) compared with the control subjects (6.9%) [OR (95% CI) = 2.19(1.49-3.22)]. In the control group, none of the haplotypes studied was associated with insulin-related indexes.


CONCLUSIONS
The PRKCZ gene variants associated with the development of T2DM in this study must be investigated in a larger population to reveal any potential effects on metabolism.
Qin
Lei
L
Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu Province, China.
Zhou
Ling
L
Wu
Xueji
X
Cheng
Jinluo
J
Wang
Jingsong
J
Du
Yunfeng
Y
Hu
Jihong
J
Shen
Moyu
M
Zhou
Jinyi
J
D044466:Asian People / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D017931:DNA Primers; D003924:Diabetes Mellitus, Type 2 / Q000201:enzymology / Q000453:epidemiology / Q000235:genetics* / Q000378:metabolism; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D007407:Interviews as Topic; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D011493:Protein Kinase C / Q000235:genetics*; D012307:Risk Factors; D011795:Surveys and Questionnaires; D000099221:Protein Kinase C zeta
D017931:DNA Primers; D011493:Protein Kinase C; D000099221:Protein Kinase C zeta