Simultaneous determination of amiodarone and its metabolite desethylamiodarone by high-performance liquid chromatography with chemiluminescent detection.
A novel method was developed for the determination of amiodarone and desethylamiodarone by high-performance liquid chromatography (HPLC) coupled with chemiluminescent (CL) detection. The procedure is based on the post-column photolysis of the analytes into photoproducts which are active in the tris(2,2'-bipyridyl)ruthenium(III) [Ru(bpy)(3)(3+)] CL system. Ru(bpy)(3)(3+) was on-line generated by photo-oxidation of the Ru(II) complex in the presence of peroxydisulfate. The separation was carried out on a Mediterranea C(18) column with isocratic elution using a mixture of methanol and 0.017 mol L(-1) ammonium sulfate buffer of pH 6.8. Under the optimum conditions, analytical curves, based on standard solutions, were linear over the range 0.1-50 microg mL(-1) for amiodarone and 0.5-25 microg mL(-1) for desethylamiodarone. The detection limits of amiodarone and desethylamiodarone were 0.02 and 0.11 microg mL(-1), respectively. Intra- and inter-day precision values of 0.9% relative standard deviation (R.S.D.) (n=10) and 1.6% R.S.D. (n=15), respectively, were obtained. The method was applied successfully to the determination of these compounds in serum and pharmaceutical formulations.
Pérez-Ruiz
Tomás
T
Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain. tpr@um.es
Martínez-Lozano
Carmen
C
García-Martínez
María Dolores
MD
D015082:2,2'-Dipyridyl / Q000031:analogs & derivatives / Q000737:chemistry; D000638:Amiodarone / Q000031:analogs & derivatives* / Q000097:blood* / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D056831:Coordination Complexes; D006801:Humans; D008163:Luminescent Measurements / Q000379:methods*; D004364:Pharmaceutical Preparations / Q000737:chemistry; D010777:Photochemistry; D012680:Sensitivity and Specificity; D013997:Time Factors; D017975:Ruthenium Compounds
D015082:2,2'-Dipyridyl; D000638:Amiodarone; D056831:Coordination Complexes; D004364:Pharmaceutical Preparations; C547232:tris(2,2-bipyridine)-ruthenium(II); D017975:Ruthenium Compounds; C036116:desethylamiodarone