Comparative analysis of renal protein expression in spontaneously hypertensive rat.
OBJECTIVE
Molecular mechanisms of nephrosclerosis caused by hypertension are not well known. Understanding changes in renal protein expression in hypertension may provide further information on how hypertension caused renal injury.


RESULTS
In the present study, we showed the protein expression profiles of the kidney in spontaneously hypertensive rats and Wistar-Kyoto rats using two-dimensional gel electrophoresis (2-DE). Differentially expressed protein spots were excised, underwent in-gel tryptic digestion, and were analyzed by MALDI-TOF MS. Eleven spots were identified. Of these identified spots, four spots were newly appeared, five spots up-regulated, and two spots down-regulated. The identified spots were mainly involved in energy metabolism, lipid transferring between membranes, and cell proliferation.


CONCLUSIONS
The expression of many proteins have changed significantly in the kidney of spontaneously hypertensive rat. NADP(+)-dependent isocitrate dehydrogenase may be a candidate for further investigation of pathophysiological mechanisms of renal injury in hypertension.
Yu
Min
M
Department of Cardiovascular Disease, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Wang
Xingxiang
X
Du
Yuxi
Y
Chen
Hongjuan
H
Guo
Xiaogang
X
Xia
Liang
L
Chen
Junzhu
J
D047228:Angiotensin II Type 1 Receptor Blockers / Q000494:pharmacology; D000818:Animals; D001160:Arterioles / Q000187:drug effects / Q000378:metabolism*; D015153:Blotting, Western; D004195:Disease Models, Animal; D015536:Down-Regulation; D015180:Electrophoresis, Gel, Two-Dimensional; D004734:Energy Metabolism / Q000235:genetics; D006973:Hypertension / Q000150:complications* / Q000188:drug therapy; D007521:Isocitrate Dehydrogenase / Q000378:metabolism; D007668:Kidney / Q000098:blood supply* / Q000187:drug effects; D019808:Losartan / Q000494:pharmacology; D008297:Male; D013058:Mass Spectrometry; D009400:Nephrosclerosis / Q000235:genetics* / Q000473:pathology; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D015854:Up-Regulation
D047228:Angiotensin II Type 1 Receptor Blockers; D007521:Isocitrate Dehydrogenase; D019808:Losartan