Succinic acid production from continuous fermentation process using Mannheimia succiniciproducens LPK7.
To achieve a higher succinic acid productivity and evaluate the industrial applicability, this study used Mannheimia succiniciproducens LPK7 (knock-out: lahA, pflB, pta-ackA), which was recently designed to enhance the productivity of succinic acid and reduce by-product secretion. Anaerobic continuous fermentation of Mannheimia succiniciproducens LPK7 was carried out at different glucose feed concentrations and dilution rates. After extensive fermentation experiments, a succinic acid yield and productivity of 0.38 mol/mol and 1.77 g/l/h, respectively, were achieved with a glucose feed concentration of 18.0 g/l and 0.2 h-1 dilution rate. A similar amount of succinic acid production was also produced in batch culture experiments. Therefore, these optimal conditions can be industrially applied for the continuous production of succinic acid. To examine the quantitative balance of the metabolism, a flux distribution analysis was also performed using the metabolic network model of glycolysis and the pentose phosphate pathway.
Oh
In Jae
IJ
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-742, Korea.
Lee
Hye Won
HW
Park
Chul Hwan
CH
Lee
Sang Yup
SY
Lee
Jinwon
J
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D005947:Glucose / Q000378:metabolism; D007218:Industrial Microbiology / Q000379:methods*; D031121:Mannheimia / Q000235:genetics / Q000378:metabolism*; D019802:Succinic Acid / Q000378:metabolism*
D001426:Bacterial Proteins; D003470:Culture Media; D019802:Succinic Acid; D005947:Glucose