A new viral RNA species in tobacco rattle virus-infected tissue.
Tobacco rattle virus (CAM strain)-infected tissue contains at least one unique species of virus-specific RNA in addition to the two virion RNAs. The new species (RNA-3; MW 0.5 x 10(6)) was characterized as follows: (1) Its synthesis and presence were detected by electrophoresis and fluorographic analysis of an extract of infected tissue that had been treated with actinomycin D and exposed to [3H]uridine; (2) its genesis from RNA-2 was determined by immobilization on specially treated diazotized paper and hybridization to cDNAs prepared to TRV RNAs 1 and 2; and (3) its template activity for a 30,000 MW protein was determined with the aid of a wheat germ-derived cell-free translation system.
Bisaro
D M
DM
Department of Biological Sciences, Wayne State University, Detroit, Michigan 48102, USA.
Siegel
A
A