Use of cyclohexanedimethanol as a nonflammable organic solvent for industrial scale reversed phase chromatography.
This work demonstrates that cyclohexanedimethanol is an effective nonflammable organic solvent for the pilot scale reversed phase chromatography of recombinant apolipoprotein A-I(Milano). Cyclohexanedimethanol has low viscosity in water, enabling the use of conventional low pressure process scale chromatography columns and hardware. Results from pilot scale manufacturing using a 30 cm diameter CG71M packed column indicate that a 5.7 log reduction in E. coli host cell protein with >80% yield can be obtained for the purification of apolipoprotein A-I(Milano).
Hunter
Alan K
AK
Pfizer Global Biologics, 700 Chesterfield Parkway West, Chesterfield, MO 63017, USA. alan.k.hunter@pfizer.com
Hoeltzli
Sydney D
SD
Johnson
Gary V
GV
Gustafson
Mark E
ME
Ho
Sa V
SV
D016632:Apolipoprotein A-I / Q000032:analysis / Q000235:genetics; D002853:Chromatography, Liquid / Q000379:methods*; D003510:Cyclohexanes / Q000737:chemistry*; D004591:Electrophoresis, Polyacrylamide Gel; D009930:Organic Chemicals / Q000737:chemistry*; D011994:Recombinant Proteins / Q000032:analysis; D012997:Solvents / Q000737:chemistry*
D016632:Apolipoprotein A-I; D003510:Cyclohexanes; D009930:Organic Chemicals; D011994:Recombinant Proteins; D012997:Solvents; C023353:1,4-cyclohexanedimethanol