title: Characterization of an inducible, antibiotic-resistant aminoacyl-tRNA synthetase gene in Streptomyces coelicolor.
abstract: Streptomyces coelicolor has two genes encoding tryptophanyl-tRNA synthetases, one of which (trpRS1) is resistant to and transcriptionally activated by indolmycin. We found that this gene also confers resistance to chuangxinmycin (another antibiotic that inhibits bacterial tryptophanyl-tRNA synthetases) and that its transcription is not absolutely dependent on either antibiotic.
authors.affiliation: Department of Chemistry, Brown University, 324 Brook Street, Providence, Rhode Island 02912, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Vecchione
authors.forename: Jason K
authors.initials: JK
authors.lastname: Sello
mesh_terms: D000604:Amino Acyl-tRNA Synthetases / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D024881:Drug Resistance, Bacterial; D015964:Gene Expression Regulation, Bacterial; D007211:Indoles / Q000494:pharmacology; D008969:Molecular Sequence Data; D048372:Streptomyces coelicolor / Q000187:drug effects / Q000201:enzymology* / Q000235:genetics; D024363:Transcription Initiation Site
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D007211:Indoles; C014414:chuangxinmycin; D000604:Amino Acyl-tRNA Synthetases; C001447:indolmycin