Scanning electron microscopic study of the in situ effect of salivary stimulation on erosion and abrasion in human and bovine enamel.
This in situ study investigated, using scanning electron microscopy, the effect of stimulated saliva on the enamel surface of bovine and human substrates submitted to erosion followed by brushing abrasion immediately or after one hour. During 2 experimental 7-day crossover phases, 9 previously selected volunteers wore intraoral palatal devices, with 12 enamel specimens (6 human and 6 bovine). In the first phase, the volunteers immersed the device for 5 minutes in 150 ml of a cola drink, 4 times a day (8h00, 12h00, 16h00 and 20h00). Immediately after the immersions, no treatment was performed in 4 specimens (ERO), 4 other specimens were immediately brushed (0 min) using a fluoride dentifrice and the device was replaced into the mouth. After 60 min, the other 4 specimens were brushed. In the second phase, the procedures were repeated but, after the immersions, the volunteers stimulated the salivary flow rate by chewing a sugar-free gum for 30 min. Enamel superficial alterations of all specimens were then evaluated using a scanning electron microscope. Enamel prism core dissolution was seen on the surfaces submitted to erosion, while on those submitted to erosion and to abrasion (both at 0 and 60 min) a more homogeneous enamel surface was observed, probably due to the removal of the altered superficial prism layer. For all the other variables--enamel substrate and salivary stimulation -, the microscopic pattern of the enamel specimens was similar.
Rios
Daniela
D
Department of Biological Sciences, School of Dentistry of Bauru, University of São Paulo, SP, Brazil.
Honório
Heitor Marques
HM
Magalhães
Ana Carolina
AC
Silva
Salete Moura Bonifácio da
SM
Delbem
Alberto Carlos Botazzo
AC
Machado
Maria Aparecida de Andrade Moreira
MA
Buzalaf
Marília Afonso Rabelo
MA
D000328:Adult; D000818:Animals; D001628:Beverages / Q000009:adverse effects; D002253:Carbonated Beverages / Q000009:adverse effects; D002417:Cattle; D002638:Chewing Gum; D003731:Dental Caries / Q000517:prevention & control; D003743:Dental Enamel / Q000187:drug effects / Q000648:ultrastructure*; D003747:Dental Enamel Solubility; D005260:Female; D005502:Food; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008297:Male; D008855:Microscopy, Electron, Scanning; D012463:Saliva / Q000737:chemistry* / Q000502:physiology; D013499:Surface Properties; D014072:Tooth Abrasion / Q000209:etiology / Q000473:pathology*; D014077:Tooth Erosion / Q000209:etiology / Q000473:pathology*; D014089:Tooth Remineralization; D014099:Toothbrushing / Q000009:adverse effects*; D055815:Young Adult
D002638:Chewing Gum