Production of the siderophore enterobactin: use of four different fermentation systems and identification of the compound by HPLC.
The article describes four different fermentation procedures for Escherichia coli AN311, a producer of enterobactin. A regular rotary shaker culture with a biphasic system consisting of an agar layer (as a reservoir for feeding processes) and a layer of liquid medium, 2.4 L and 10 L batch cultures, and a novel dialysis membrane fermentor were used. With the use of this latter fermentor type, the production of enterobactin could be increased by a factor of about 9.5, while growth increased by a factor of 12 compared to the other systems. For the rapid and reliable quantification of the concentration and purity of enterobactin an analytical and preparative high-performance liquid chromatography (HPLC) method was established. The degradation compounds of this siderophore were detected by diodearray and bioassays. A comparison of total catechol production as well as the distribution between enterobactin and its degradation compounds is given.
Seiffert
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Biologisches institut, LB Mikrobiologie/Antibiotika, Universität Tübingen, auf der Morgenstelle 28, 7400 Tübingen, Germany.
Goeke
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Fielder
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Zähner
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