Evaluation and quantification of nuclear DNA from human telogen hairs.
Nuclear DNA was extracted from human telogen hairs from 60 individuals. Six to nine hairs from each individual were individually extracted. The amount of DNA recovered from each individual varied greatly, and most samples yielded a quantity of 550 pg or less per hair. A selective extraction buffer was used to remove epithelial cell DNA and the amount of exogenous DNA was determined. DNA was also quantified by real time PCR using three different sized amplicons targeting an Alu sequence. The results were used to determine the state of degradation of the extracted DNA. Different quantities of sample (<100 pg, 100-500 pg, >500 pg) were amplified with the Miniplex kits to determine the minimum DNA template required for successful amplification. DNA recovered from hair showed degradation; however, partial profiles were obtained for those samples containing at least 60 pg using MiniSTRs.
Opel
Kerry L
KL
Department of Chemistry and Biochemistry, Florida International University, University Park, 11200 SW 8th Street, Miami, FL 33199, USA.
Fleishaker
Erica L
EL
Nicklas
Janice A
JA
Buel
Eric
E
McCord
Bruce R
BR
D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting / Q000379:methods*; D017931:DNA Primers; D006197:Hair / Q000737:chemistry* / Q000254:growth & development; D006801:Humans; D016133:Polymerase Chain Reaction; D020080:Tandem Repeat Sequences
D017931:DNA Primers; D004247:DNA