The crystal structure of coxsackievirus B3 RNA-dependent RNA polymerase in complex with its protein primer VPg confirms the existence of a second VPg binding site on Picornaviridae polymerases.
The RNA-dependent RNA polymerase (RdRp) is a central piece in the replication machinery of RNA viruses. In picornaviruses this essential RdRp activity also uridylates the VPg peptide, which then serves as a primer for RNA synthesis. Previous genetic, binding, and biochemical data have identified a VPg binding site on poliovirus RdRp and have shown that is was implicated in VPg uridylation. More recent structural studies have identified a topologically distinct site on the closely related foot-and-mouth disease virus RdRp supposed to be the actual VPg-primer-binding site. Here, we report the crystal structure at 2.5-A resolution of active coxsackievirus B3 RdRp (also named 3D(pol)) in a complex with VPg and a pyrophosphate. The pyrophosphate is situated in the active-site cavity, occupying a putative binding site either for the coproduct of the reaction or an incoming NTP. VPg is bound at the base of the thumb subdomain, providing first structural evidence for the VPg binding site previously identified by genetic and biochemical methods. The binding mode of VPg to CVB3 3D(pol) at this site excludes its uridylation by the carrier 3D(pol). We suggest that VPg at this position is either uridylated by another 3D(pol) molecule or that it plays a stabilizing role within the uridylation complex. The CVB3 3D(pol)/VPg complex structure is expected to contribute to the understanding of the multicomponent VPg-uridylation complex essential for the initiation of genome replication of picornaviruses.
Gruez
Arnaud
A
Architecture et Fonction des Macromolécules Biologiques, CNRS and Universités d'Aix-Marseille I et II, UMR 6098, ESIL Case 925, 13288 Marseille, France.
Selisko
Barbara
B
Roberts
Michael
M
Bricogne
Gérard
G
Bussetta
Cécile
C
Jabafi
Ilham
I
Coutard
Bruno
B
De Palma
Armando M
AM
Neyts
Johan
J
Canard
Bruno
B
D000595:Amino Acid Sequence; D001665:Binding Sites; D018360:Crystallography, X-Ray / Q000379:methods; D011756:Diphosphates / Q000737:chemistry; D004770:Enterovirus / Q000201:enzymology* / Q000235:genetics; D016679:Genome, Viral; D006160:Guanosine Triphosphate / Q000737:chemistry; D008968:Molecular Conformation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry; D010849:Picornaviridae / Q000201:enzymology*; D011485:Protein Binding; D012324:RNA-Dependent RNA Polymerase / Q000737:chemistry*; D017386:Sequence Homology, Amino Acid; D014779:Virus Replication
D011756:Diphosphates; D010455:Peptides; D006160:Guanosine Triphosphate; D012324:RNA-Dependent RNA Polymerase