Engineering and assaying of cytochrome P450 biocatalysts.
Cytochrome P450s constitute a highly fascinating superfamily of enzymes which catalyze a broad range of reactions. They are essential for drug metabolism and promise industrial applications in biotechnology and biosensing. The constant search for cytochrome P450 enzymes with enhanced catalytic performances has generated a large body of research. This review will concentrate on two key aspects related to the identification and improvement of cytochrome P450 biocatalysts, namely the engineering and assaying of these enzymes. To this end, recent advances in cytochrome P450 development are reported and commonly used screening methods are surveyed.
Rabe
Kersten S
KS
Fakultät für Chemie, Biologisch-Chemische Mikrostrukturtechnik, Technische Universität Dortmund, Otto-Hahn-Strabetae 6, 44227, Dortmund, Germany.
Gandubert
Valérie J
VJ
Spengler
Mark
M
Erkelenz
Michael
M
Niemeyer
Christof M
CM
D000818:Animals; D001665:Binding Sites; D001681:Biological Assay / Q000379:methods*; D002384:Catalysis; D003577:Cytochrome P-450 Enzyme System / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D009249:NADP / Q000378:metabolism; D010084:Oxidation-Reduction; D010101:Oxygen Consumption; D010545:Peroxides / Q000737:chemistry / Q000378:metabolism; D015202:Protein Engineering / Q000379:methods*; D017382:Reactive Oxygen Species / Q000378:metabolism; D013379:Substrate Specificity
D010545:Peroxides; D017382:Reactive Oxygen Species; D009249:NADP; D003577:Cytochrome P-450 Enzyme System