HC-Pro protein of sugar cane mosaic virus interacts specifically with maize ferredoxin-5 in vitro and in planta.
Symptom development of a plant viral disease is a result of molecular interactions between the virus and its host plant; thus, the elucidation of specific interactions is a prerequisite to reveal the mechanism of viral pathogenesis. Here, we show that the chloroplast precursor of ferredoxin-5 (Fd V) from maize (Zea mays) interacts with the multifunctional HC-Pro protein of sugar cane mosaic virus (SCMV) in yeast, Nicotiana benthamiana cells and maize protoplasts. Our results demonstrate that the transit peptide rather than the mature protein of Fd V precursor could interact with both N-terminal (residues 1-100) and C-terminal (residues 301-460) fragments, but not the middle part (residues 101-300), of HC-Pro. In addition, SCMV HC-Pro interacted only with Fd V, and not with the other two photosynthetic ferredoxin isoproteins (Fd I and Fd II) from maize plants. SCMV infection significantly downregulated the level of Fd V mRNA in maize plants; however, no obvious changes were observed in levels of Fd I and Fd II mRNA. These results suggest that SCMV HC-Pro interacts specifically with maize Fd V and that this interaction may disturb the post-translational import of Fd V into maize bundle-sheath cell chloroplasts, which could lead to the perturbation of chloroplast structure and function.
Cheng
Yu-Qin
YQ
Department of Pomology, China Agricultural University, Beijing 100094, PR China.|Department of Plant Pathology and State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100094, PR China.
Liu
Zhong-Mei
ZM
Department of Plant Pathology and State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100094, PR China.
Xu
Jian
J
Department of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, PR China.
Zhou
Tao
T
Department of Plant Pathology and State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100094, PR China.
Wang
Meng
M
Department of Pomology, China Agricultural University, Beijing 100094, PR China.|Department of Plant Pathology and State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100094, PR China.
Chen
Yu-Ting
YT
Department of Plant Pathology and State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100094, PR China.
Li
Huai-Fang
HF
Department of Plant Pathology and State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100094, PR China.
Fan
Zai-Feng
ZF
Department of Plant Pathology and State Key Laboratory of Agro-Biotechnology, China Agricultural University, Beijing 100094, PR China.
D000595:Amino Acid Sequence; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D005288:Ferredoxins / Q000235:genetics / Q000378:metabolism*; D005786:Gene Expression Regulation; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D017800:Potyvirus / Q000378:metabolism / Q000472:pathogenicity*; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism*; D011523:Protoplasts / Q000378:metabolism; D031786:Saccharum / Q000821:virology*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D014026:Nicotiana / Q000378:metabolism; D014764:Viral Proteins / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism / Q000821:virology*
D005288:Ferredoxins; D010940:Plant Proteins; D011498:Protein Precursors; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; C072775:HC-Pro protein, potyvirus