In vitro study on influence of nano particles of CuO on CA1 pyramidal neurons of rat hippocampus potassium currents.
The effects of nano particles of CuO on voltage-dependent potassium currents were studied in acutely isolated CA1 pyramidal neurons of rat hippocampus using the whole-cell patch-clamp techniques. Nano particles of CuO had small effects on transient outward potassium current (I(A), no statistical significance) and mainly inhibited delayed rectifier potassium current (I(K)) in the concentration of 5 x 10(-5) g/mL. Nano particles of CuO didn't shift the steady-state activation curve of I(K) and I(A) but negatively shifted the inactivation curve of I(K). The effects on inactivation curve of I(A) had no statistical significance. These results suggested that blockades of K+ currents by nano particles of CuO could be preferential for I(k) for the first time. This may interfere with the normal function of nerve cells.
Xu
Lan-Ju
LJ
College of Medicine, Nankai University, Tianjin 300071, China.
Zhao
Jing-Xia
JX
Zhang
Tao
T
Ren
Guo-Gang
GG
Yang
Zhuo
Z
D000818:Animals; D002478:Cells, Cultured; D003300:Copper / Q000494:pharmacology*; D051668:Delayed Rectifier Potassium Channels / Q000037:antagonists & inhibitors*; D004553:Electric Conductivity; D006624:Hippocampus / Q000166:cytology / Q000187:drug effects* / Q000502:physiology; D007700:Kinetics; D053768:Metal Nanoparticles; D011188:Potassium / Q000378:metabolism; D024642:Potassium Channels, Voltage-Gated / Q000037:antagonists & inhibitors*; D017966:Pyramidal Cells / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
D051668:Delayed Rectifier Potassium Channels; D024642:Potassium Channels, Voltage-Gated; D003300:Copper; D011188:Potassium; C030973:cupric oxide