title: Rapid detection of multidrug-resistant Mycobacterium tuberculosis in Cotonou (Benin) using two low-cost colorimetric methods: resazurin and nitrate reductase assays.
abstract: We have evaluated two simple, rapid and low-cost colorimetric methods for the detection of multidrug-resistant Mycobacterium tuberculosis. A total of 151 M. tuberculosis strains were tested for resistance to rifampicin (RMP) and isoniazid by resazurin microplate assay (REMA) and nitrate reductase assay (NRA) in comparison with the conventional proportion method (PM) on Löwenstein-Jensen medium. A complete agreement was found between NRA and PM, while one false RMP-susceptible result was found by REMA. REMA and NRA tests are rapid and inexpensive, and could be good alternatives to the conventional PM in low-resource countries.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.|Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
authors.forename: Dissou
authors.initials: D
authors.lastname: Affolabi
authors.affiliation: Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
authors.forename: N'Dira
authors.initials: N
authors.lastname: Sanoussi
authors.affiliation: Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Odoun
authors.affiliation: Médecins Sans Frontières, 75011 Paris, France.|Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.
authors.forename: Anandi
authors.initials: A
authors.lastname: Martin
authors.affiliation: Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
authors.forename: Louis
authors.initials: L
authors.lastname: Koukpemedji
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Palomino
authors.affiliation: Immnunology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.
authors.forename: Luc
authors.initials: L
authors.lastname: Kestens
authors.affiliation: Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
authors.forename: Séverin
authors.initials: S
authors.lastname: Anagonou
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.
authors.forename: Françoise
authors.initials: F
authors.lastname: Portaels
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism; D001541:Benin; D003124:Colorimetry; D024881:Drug Resistance, Bacterial; D018432:Drug Resistance, Multiple / Q000502:physiology*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D050901:Nitrate Reductase / Q000378:metabolism*; D012293:Rifampin / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D050901:Nitrate Reductase; D012293:Rifampin