Notochordal cells stimulate migration of cartilage end plate chondrocytes of the intervertebral disc in in vitro cell migration assays.
BACKGROUND
It was recently demonstrated that the postnatal transition from a notochordal to a fibrocartilaginous nucleus pulposus (NP) is accomplished exogenously by chondrocytes migrating from hyaline cartilage end plates (CEs) into the ectopic notochordal NP region. Although our previous in vivo studies showed evidences for the migration of CE chondrocyte from hyaline CEs into the notochordal NP, it is unknown whether CE chondrocytes of the intervertebral disc (IVD) really have a motile property. In addition, the effect of notochordal cells on this property has not been elucidated.


OBJECTIVE
The purpose of this in vitro study was to demonstrate whether CE chondrocytes of the IVD are capable of migration, and whether there is any biological link between notochordal cells and CE chondrocytes that may regulate the CE chondrocyte migration.


METHODS
In vitro cell migration assays were performed using rat IVDs.


METHODS
Notochordal cells and chondrocytes were obtained from the NP and CE tissues, respectively, and were cultured separately. The different numbers of notochordal cells and the supernatant (conditioned medium) that contained soluble factors produced by notochordal cells were used to demonstrate their effects on the migration of CE chondrocytes. Bovine serum albumin (BSA) and lysophosphatidic acid (LPA) were used as negative and positive controls, respectively.


RESULTS
Compared with BSA, LPA, notochordal cells (N=4x, 2x, 1x, and 0.5 x 10(5)), and its conditioned media (unconcentrated and fivefold concentrated) significantly increased migration of CE chondrocytes (p<.05 in all comparisons). Particularly, notochordal cells and its conditioned media increased migration in a number- and concentration-dependent manner, respectively.


CONCLUSIONS
This study demonstrates that CE chondrocytes of the IVD are capable of migration and that soluble factors produced by notochordal cells stimulate the migration. These results provide a plausible explanation to the question of why CE chondrocytes of the IVD migrate into the ectopic NP region during the natural transition from the notochordal to fibrocartilaginous NP.
Kim
Ki-Won
KW
Department of Orthopedic Surgery, St Mary's Hospital, The Catholic University of Korea, Seoul, Korea. kiwonk@lycos.co.kr
Ha
Kee-Yong
KY
Lee
Jun-Seok
JS
Nam
Suk-Woo
SW
Woo
Young-Kyun
YK
Lim
Tae-Hong
TH
An
Howard S
HS
D000818:Animals; D002356:Cartilage / Q000166:cytology; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology*; D002633:Chemotaxis / Q000187:drug effects / Q000502:physiology; D019902:Chondrocytes / Q000166:cytology* / Q000187:drug effects; D017077:Culture Media, Conditioned / Q000494:pharmacology; D006132:Growth Plate / Q000166:cytology / Q000196:embryology; D066298:In Vitro Techniques; D007403:Intervertebral Disc / Q000166:cytology* / Q000196:embryology; D008297:Male; D009672:Notochord / Q000166:cytology* / Q000196:embryology; D051381:Rats; D017208:Rats, Wistar; D012995:Solubility
D017077:Culture Media, Conditioned