title: Effect of sample quality on the sensitivity of endoscopic biopsy for detecting gastric and duodenal lesions in dogs and cats.
abstract: BACKGROUND
  The quality of histopathology slides of endoscopic biopsies from different laboratories varies, but the effect of biopsy quality on outcome is unknown.
  
  
  OBJECTIVE
  The ability to demonstrate a histologic lesion in the stomach or duodenum of a dog or cat is affected by the quality of endoscopic biopsy samples submitted. More endoscopic samples are needed to find a lesion in poor-quality tissue specimens.
  
  
  METHODS
  Tissues from 99 dogs and 51 cats were examined as clinical cases at 8 veterinary institutions or practices in 5 countries.
  
  
  METHODS
  Histopathology slides from sequential cases that underwent endoscopic biopsy were submitted by participating institutions. Quality of the histologic section of tissue (inadequate, marginal, adequate), type of lesion (lymphangiectasia, crypt lesion, villus blunting, cellular infiltrate), and severity of lesion (normal, mild, moderate, severe) were determined. Sensitivity of different quality tissue samples for finding different lesions was determined.
  
  
  RESULTS
  Fewer samples were required from dogs for diagnosis as the quality of the sample improved from inadequate to marginal to adequate. Duodenal lesions in cats displayed the same trend except for moderate duodenal infiltrates for which quality of tissue sample made no difference. Gastric lesions in dogs and mild gastric lesions in cats had the same trend, whereas the number of tissue samples needed to diagnose moderately severe gastric lesions in cats was not affected by the quality of tissue sample.
  
  
  CONCLUSIONS
  The quality of endoscopically obtained tissue samples has a profound effect on their sensitivity for identifying certain lesions, and there are differences between biopsies of canine and feline tissues.
authors.affiliation: Department of Small Animal Clinical Sciences, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843-4474, USA. mwillard@cvm.tamu.edu
authors.forename: M D
authors.initials: MD
authors.lastname: Willard
authors.forename: J
authors.initials: J
authors.lastname: Mansell
authors.forename: G T
authors.initials: GT
authors.lastname: Fosgate
authors.forename: M
authors.initials: M
authors.lastname: Gualtieri
authors.forename: D
authors.initials: D
authors.lastname: Olivero
authors.forename: P
authors.initials: P
authors.lastname: Lecoindre
authors.forename: D C
authors.initials: DC
authors.lastname: Twedt
authors.forename: M G
authors.initials: MG
authors.lastname: Collett
authors.forename: M J
authors.initials: MJ
authors.lastname: Day
authors.forename: E J
authors.initials: EJ
authors.lastname: Hall
authors.forename: A E
authors.initials: AE
authors.lastname: Jergens
authors.forename: J W
authors.initials: JW
authors.lastname: Simpson
authors.forename: R W
authors.initials: RW
authors.lastname: Else
authors.forename: R J
authors.initials: RJ
authors.lastname: Washabau
mesh_terms: D000818:Animals; D001706:Biopsy / Q000379:methods / Q000662:veterinary*; D002371:Cat Diseases / Q000175:diagnosis*; D002415:Cats; D004283:Dog Diseases / Q000175:diagnosis*; D004285:Dogs; D004378:Duodenal Diseases / Q000175:diagnosis / Q000662:veterinary*; D004724:Endoscopy / Q000379:methods / Q000662:veterinary*; D012680:Sensitivity and Specificity; D013272:Stomach Diseases / Q000175:diagnosis / Q000662:veterinary*