Bioavailability of carotenoids and alpha-tocopherol from fruit juices in the presence of absorption modifiers: in vitro and in vivo assessment.
The food industry is playing an increasing role in the development and marketing of new products although little is known regarding the bioavailability of the phytochemicals they contain. Our aim was to assess the effect of the presence of absorption modifiers (milk and iron) on the in vitro bioaccessibility and the serum response in vivo of carotenoids and alpha-tocopherol from fruit juices. Thirty-two young women participated in a three-period (21 d each) supplementation study with a 2-week wash-out in between. Subjects consumed consecutively 2 x 250 ml/d vitamin C-fortified juices supplied as fruit juice, fruit juice containing milk and fruit juice containing milk and iron. Fasting blood samples were collected before and after each supplementation period. In vitro bioaccessibility of carotenoids and alpha-tocopherol was assessed by a static digestion model. Vitamin E and carotenoids from both studies were determined by HPLC. In vitro, xanthophyll ester hydrolysis and transference of free xanthophylls and alpha-tocopherol into the micellar phase were higher in the presence of absorption modifiers. In vivo, consumption of the fruit juices provoked significant increments (within-subject) of alpha-tocopherol and some carotenoids in serum. Dose-adjusted increments in serum of some carotenoids were higher when subjects consumed juices with milk and milk plus iron, although differences did not reach statistical significance. In conclusion, the presence of milk and milk plus iron do not influence the bioavailability of carotenoids and alpha-tocopherol from fruit juices in vivo. Our results support the use of in vitro models to assess food-related factors affecting bioavailability of carotenoids and tocopherols from foods.
Granado-Lorencio
F
F
Unidad de Vitaminas, Servicio de Bioquímica Clínica, Hospital Universitario Puerta de Hierro, c/ San Martín de Porres, 4, 28035 Madrid, Spain. fgranado.hpth@salud.madrid.org
Herrero-Barbudo
C
C
Blanco-Navarro
I
I
Pérez-Sacristán
B
B
Olmedilla-Alonso
B
B
D000328:Adult; D000704:Analysis of Variance; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000493:pharmacokinetics*; D001628:Beverages; D001682:Biological Availability; D002338:Carotenoids / Q000008:administration & dosage / Q000493:pharmacokinetics*; D004386:Duodenum / Q000378:metabolism*; D004951:Esterification; D005260:Female; D005638:Fruit; D006801:Humans; D066298:In Vitro Techniques; D007408:Intestinal Absorption / Q000502:physiology; D007501:Iron / Q000008:administration & dosage; D008823:Micelles; D008892:Milk; D014815:Vitamins / Q000008:administration & dosage* / Q000378:metabolism; D024502:alpha-Tocopherol / Q000008:administration & dosage / Q000493:pharmacokinetics*
D000975:Antioxidants; D008823:Micelles; D014815:Vitamins; D002338:Carotenoids; D007501:Iron; D024502:alpha-Tocopherol