title: Interactions of Sox10 and Egr2 in myelin gene regulation.
abstract: Myelination in the PNS is accompanied by a large induction of the myelin protein zero (Mpz) gene to produce the most abundant component in peripheral myelin. Analyses of knockout mice have shown that the EGR2/Krox20 and SOX10 transcription factors are required for Mpz expression. Our recent work has shown that the dominant EGR2 mutations associated with human peripheral neuropathies cause disruption of EGR2/SOX10 synergy at specific sites, including a conserved enhancer element in the first intron of the Mpz gene. Further investigation of Egr2/Sox10 interactions reveals that activation of the Mpz intron element by Egr2 requires both Sox10-binding sites. In addition, both Egr1 and Egr3 cooperate with Sox10 to activate this element, which indicates that this capacity is conserved among Egr family members. Finally, a conserved composite structure of Egr2/Sox10-binding sites in the genes encoding Mpz, myelin-associated glycoprotein and myelin basic protein genes was used to screen for similar modules in other myelin genes, revealing a potential regulatory element in the periaxin gene. Overall, these results elucidate a working model for developmental regulation of Mpz expression, several facets of which extend to regulation of other peripheral myelin genes.
authors.affiliation: Program in Cellular and Molecular Biology, University of Wisconsin-Madison, Madison, WI 53706, USA. jpsvaren@wisc.edu
authors.forename: Erin A
authors.initials: EA
authors.lastname: Jones
authors.forename: Sung-Wook
authors.initials: SW
authors.lastname: Jang
authors.forename: Gennifer M
authors.initials: GM
authors.lastname: Mager
authors.forename: Li-Wei
authors.initials: LW
authors.lastname: Chang
authors.forename: Rajini
authors.initials: R
authors.lastname: Srinivasan
authors.forename: Nolan G
authors.initials: NG
authors.lastname: Gokey
authors.forename: Rebecca M
authors.initials: RM
authors.lastname: Ward
authors.forename: Rakesh
authors.initials: R
authors.lastname: Nagarajan
authors.forename: John
authors.initials: J
authors.lastname: Svaren