[The primary evaluation of Sal-Ammoniac extract on the therapy action in mice bearing Lewis lung cancer].
OBJECTIVE
To evaluate the therapy action of Sal-Ammoniac extract on Lewis lung cancer and its toxicity to immunity.


METHODS
The proliferation and cell cycle of Lewis lung cancer cells were determined by MTT assay and flow cytometry respectively. The antitumor effect of Sal-Ammoniac extract was observed by tumor injected subcutaneously in mice and its toxicity to immunity was examined by clearance rate of charcoal particles and delayed type hypersensitivity.


RESULTS
Sal-Ammoniac extract could inhibit the proliferation of Lewis lung cancer cells with S cell cycle arrest in a dose-dependent manner in vitro. Sal-Ammoniac extract solution injected in tumor for eight days had 46.7% inhibition on Lewis lung cancer, if taken orally had only 15.7% inhibition on Lewis lung cancer in mice. Sal-Ammoniac extract solution injected subcutaneously or taken orally had no effect on the clearance rate of charcoal particles and delayed type hypersensitivity in mice.


CONCLUSIONS
The antitumor action of Sal-Ammoniac extract has relation to its recipe and has no influence on immunity.
Han
Xiao-Fen
XF
Pharmaceutical College of Henan University, Kaifeng 475001 ,China.
Du
Gang-Jun
GJ
Lin
Hai-Hong
HH
Song
Zi-Hui
ZH
Wang
Mei
M
Zhang
Shuo
S
D000643:Ammonium Chloride / Q000008:administration & dosage / Q000494:pharmacology*; D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage / Q000494:pharmacology*; D018827:Carcinoma, Lewis Lung / Q000188:drug therapy* / Q000473:pathology; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical; D005260:Female; D005434:Flow Cytometry; D008419:Materia Medica / Q000008:administration & dosage / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL
D000970:Antineoplastic Agents; D008419:Materia Medica; D000643:Ammonium Chloride