title: Resin comparison and fast automated stepwise conventional synthesis of human SDF-1alpha.
abstract: Human SDF-1alpha contains 68 amino acids and is a member of the chemokine family of peptides. This long peptide was synthesized stepwise using classical conditions in 101 h. The reaction times were then reduced to deprotection times of 2 x 2 min and coupling times of 2 x 2.5 min, resulting in a total synthesis time of 22 h. The effect of different resins, resin substitutions and deprotection reagents on the crude peptide purities was compared. A small portion of crude peptide was purified using an RP-HPLC column and the mass of the final product was confirmed with MALDI-TOF mass spectrometry.
authors.affiliation: Department of Chemistry, Protein Technologies Inc., 4675, South Coach Drive, Tucson, Arizona 85714, USA.
authors.forename: Hirendra
authors.initials: H
authors.lastname: Patel
authors.forename: Christina A
authors.initials: CA
authors.lastname: Chantell
authors.forename: German
authors.initials: G
authors.lastname: Fuentes
authors.forename: Mahendra
authors.initials: M
authors.lastname: Menakuru
authors.forename: Jae H
authors.initials: JH
authors.lastname: Park
mesh_terms: D002625:Chemistry, Organic / Q000379:methods*; D054377:Chemokine CXCL12 / Q000138:chemical synthesis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012117:Resins, Synthetic / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D054377:Chemokine CXCL12; D012117:Resins, Synthetic