Acanthamoeba spp. cysts storage in filter paper.
A new method of storage of the free-living amoeba, Acanthamoeba, is described. For this purpose, strips of filter paper were impregnated with cysts of amoebas cultivated axenically and transferred into a cryotube. The strips were dehydrated at 30 degrees C for 24 h and after storage at room temperature. The amoeba viability has been evaluated and the cysts remain viable and pure for at least 1 year. Then, our method has been efficient for the storage of cysts during the evaluated period.
Pens
Claiton José
CJ
Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. claitonjp@hotmail.com
Rott
Marilise Brittes
MB
D000048:Acanthamoeba / Q000502:physiology*; D000818:Animals; D010209:Paper; D011309:Preservation, Biological; D013997:Time Factors