title: Notch-regulated oligodendrocyte specification from radial glia in the spinal cord of zebrafish embryos.
abstract: During vertebrate neural development, many dividing neuroepithelial precursors adopt features of radial glia, which are now known to also serve as neural precursors. In mammals, most radial glia do not persist past early postnatal stages, whereas zebrafish maintain large numbers of radial glia into adulthood. The mechanisms that maintain and specify radial glia for different fates are still poorly understood. We investigated formation of radial glia in the spinal cord of zebrafish and the role of Notch signaling in their maintenance and specification. We found that spinal cord precursors begin to express gfap+, a marker of radial glia, during neurogenesis and that gfap cells give rise to both neurons and oligodendrocytes. We also determined that Notch signaling is continuously required during embryogenesis to maintain radial glia, limit motor neuron formation and permit oligodendrocyte development, but that radial glia seem to be refractory to changes in Notch activity in postembryonic animals.
authors.affiliation: Graduate School of Medicine, Korea University, Ansan, Gyeonggido, Republic of Korea.
authors.forename: Ho
authors.initials: H
authors.lastname: Kim
authors.forename: Jimann
authors.initials: J
authors.lastname: Shin
authors.forename: Suhyun
authors.initials: S
authors.lastname: Kim
authors.forename: Justin
authors.initials: J
authors.lastname: Poling
authors.forename: Hae-Chul
authors.initials: HC
authors.lastname: Park
authors.forename: Bruce
authors.initials: B
authors.lastname: Appel
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics / Q000378:metabolism; D019070:Cell Lineage / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D009046:Motor Neurons / Q000166:cytology / Q000502:physiology; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D000076104:Oligodendrocyte Transcription Factor 2; D009836:Oligodendroglia / Q000166:cytology* / Q000502:physiology; D051881:Receptor, Notch1 / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology* / Q000196:embryology* / Q000502:physiology; D013234:Stem Cells / Q000166:cytology / Q000502:physiology; D015027:Zebrafish / Q000196:embryology* / Q000502:physiology; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D051792:Basic Helix-Loop-Helix Proteins; D005904:Glial Fibrillary Acidic Protein; D018398:Homeodomain Proteins; D009419:Nerve Tissue Proteins; D000076104:Oligodendrocyte Transcription Factor 2; D051881:Receptor, Notch1; D029961:Zebrafish Proteins; C437805:notch1a protein, zebrafish; C465361:olig2 protein, zebrafish