title: Pilot and plant scaled production of ACE inhibitory hydrolysates  from Acetes chinensis and its in vivo antihypertensive effect.
abstract: The angiotensin-I-converting enzyme (ACE) inhibitory oligopeptide-enriched hydrolysates from Acetes chinensis by treatment with the protease from Bacillus sp. SM98011 were produced at pilot scale (100 L) and plant scale (1000 L). The pilot and plant scaled hydrolysate products almost had the same properties as that at laboratory scale. Spray-drying had little effect on the peptide composition and bioactivity of the hydrolysates. The plant scaled hydrolysates were used to study its blood pressure-depressing effect in vivo. It caused reduce of 18.3-38.6 mmHg of the blood pressure of spontaneously hypertensive rats in dose-dependent manner in the range of 100-1,200 mg/kg/day. Histopathologic study showed that the pathologic changes of heart and brain in SHRs got obvious alleviation after treatment of the hydrolysates.
authors.affiliation: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Jinan 250100, China.
authors.forename: Hai-Lun
authors.initials: HL
authors.lastname: He
authors.forename: Hao
authors.initials: H
authors.lastname: Wu
authors.forename: Xiu-Lan
authors.initials: XL
authors.lastname: Chen
authors.forename: Mei
authors.initials: M
authors.lastname: Shi
authors.forename: Xi-Ying
authors.initials: XY
authors.lastname: Zhang
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Sun
authors.forename: Yu-Zhong
authors.initials: YZ
authors.lastname: Zhang
authors.forename: Bai-Cheng
authors.initials: BC
authors.lastname: Zhou
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000378:metabolism* / Q000627:therapeutic use; D000818:Animals; D000959:Antihypertensive Agents; D001921:Brain / Q000187:drug effects / Q000473:pathology; D003445:Crustacea; D006321:Heart / Q000187:drug effects; D006868:Hydrolysis; D006973:Hypertension / Q000188:drug therapy* / Q000473:pathology; D008297:Male; D009206:Myocardium / Q000473:pathology; D009842:Oligopeptides / Q000737:chemistry / Q000302:isolation & purification; D007703:Peptidyl-Dipeptidase A; D010865:Pilot Projects; D051381:Rats; D011918:Rats, Inbred SHR
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D009842:Oligopeptides; D007703:Peptidyl-Dipeptidase A