title: Loss of antibody productivity is highly reproducible in multiple hybridoma subclones.
abstract: An immunoglobulin G (IgG(2b)) producing hybridoma cell line (S3H5/gamma2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation to serum-free medium, it was found that 16 of the 20 subclones remained at a relatively constant proportion of nonproducing cells. Three of the remaining subclones transiently deviated from this balance but eventually returned toward this population composition. One subclone continued to lose productivity. A population balance was reached at approximately 8% of the population being nonproducers. The loss of antibody productivity was thus highly reproducible.
authors.affiliation: Cellular Biotechnology Laboratory, Department of Chemical Engineering, The University of Michigan, Ann Arbor, Michigan 48109, USA.
authors.forename: S E
authors.initials: SE
authors.lastname: Merritt
authors.forename: B O
authors.initials: BO
authors.lastname: Palsson