title: Characterization of ISApl1, an insertion element identified from Actinobacillus pleuropneumoniae field isolate in China.
abstract: An insertion sequence (IS), designated ISApl1, was identified in Actinobacillus pleuropneumoniae. It was 1072 bp in length, and contained a large open reading frame (ORF), which encoded a putative transposase whose sequence was similar to that of transposases of various IS elements of the IS30 family. Another small ORF, a putative antisense repressor of transposase, was located in the opposite direction of transposase. ISApl1 generated a 3-bp duplication of the target DNA and carried 24-bp inverted repeats sequence. ISApl1 was identified in the genome of a biofilm-formation negative A. pleuropneumoniae strain field isolate HB04 and inserted into an A/T rich region of the ORF of pgaC, which encoded the PGA [N-acetyl-D-glucosamine residues in beta(1,6) linkage] synthesizing N-glycosyltransferase. The genotype of the pgaC(-)/IS(+) was not altered after re-isolation from challenged mice, which indicated that this IS element was relatively stable in A. pleuropneumoniae during infection.
authors.affiliation: Division of Animal Infectious Disease, State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Jinlin
authors.initials: J
authors.lastname: Liu
authors.forename: Chen
authors.initials: C
authors.lastname: Tan
authors.forename: Jinquan
authors.initials: J
authors.lastname: Li
authors.forename: Huiwen
authors.initials: H
authors.lastname: Chen
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.forename: Qigai
authors.initials: Q
authors.lastname: He
authors.forename: Weicheng
authors.initials: W
authors.lastname: Bei
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
mesh_terms: D016977:Actinobacillus pleuropneumoniae / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D018441:Biofilms / Q000254:growth & development; D002681:China; D004251:DNA Transposable Elements / Q000235:genetics*; D004269:DNA, Bacterial / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005816:Genetic Complementation Test; D008969:Molecular Sequence Data; D019895:Transposases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D019895:Transposases