Candida albicans infection delays duodenal ulcer healing in cysteamine-induced duodenal ulcers in rats.
A low curability of ulcers infected with Candida has been reported in the literature. The aim of the study reported here was to investigate experimentally whether Candida infection affects the healing of ulcers. Candida albicans (the Candida group) or saline (the control group) was administered intragastrically into rats with a cysteamine-induced duodenal ulcer. The duodenal lesions, vascular endothelial growth factor A (VEGF-A) and proliferating cell nuclear antigen (PCNA) were assessed. On Day 7 post-administration, 70.4% rats of the Candida group had a duodenal ulcer compared with 33.3% in the control group (P < 0.05). The duodenal ulcer in the Candida group was significantly larger and deeper than that in the control group. The number of VEGF-A- and PCNA-positive cells was smaller and the area of VEGF-A expression was lower in the Candida group. Using a rat model, we have demonstrated that Candida infection can delay the wound healing process of duodenal ulcers by means of a low expression of VEGF-A and PCNA.
Jin
Longxue
L
Department of Surgery, Keio University School of Medicine, Shinjuku-ku, Tokyo, 160-8582, Japan.
Yoshida
Masashi
M
Nakamura
Tetsuya
T
Ishikawa
Hideki
H
Wakabayashi
Go
G
Tanabe
Minoru
M
Kawachi
Shigeyuki
S
Shinoda
Masahiro
M
Saikawa
Yoshiro
Y
Wada
Norihito
N
Kameyama
Kaori
K
Kumai
Koichiro
K
Kubota
Tetsuro
T
Sano
Katsuko
K
Nagao
Keisuke
K
Amagai
Masayuki
M
Kitagawa
Yuko
Y
Kitajima
Masaki
M
D000818:Animals; D002176:Candida albicans / Q000472:pathogenicity*; D002177:Candidiasis / Q000150:complications*; D003543:Cysteamine; D004195:Disease Models, Animal; D004381:Duodenal Ulcer / Q000139:chemically induced / Q000382:microbiology* / Q000503:physiopathology*; D004386:Duodenum / Q000378:metabolism / Q000382:microbiology / Q000473:pathology; D008297:Male; D018809:Proliferating Cell Nuclear Antigen / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D013997:Time Factors; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
D018809:Proliferating Cell Nuclear Antigen; D042461:Vascular Endothelial Growth Factor A; D003543:Cysteamine