[Study on transdermal absorption characteristics of brucine in vitro].
OBJECTIVE
To study the transdermal absorption characteristics of brucine in vitro.


METHODS
The oil-water partition coefficient of brucine was measured. Drug permeation tests were performed through excised rat skin in improved Franz diffusion cell. Brucine concentration in samples was determined by HPLC.


RESULTS
The oil-water partition coefficient of brucine was between 4.05 and 5.02 at different temperatures. The permeation rate of 0.5 and 1 mg/ml brucine solution were (1.83 +/- 0.85) and (3.74 +/- 1.54) microg/cm2 x h, respectively. The cumulative permeation ratio in 24 hours were (51.30 +/- 18.51)% and (50.01 +/- 12. 80)%, respectively.


CONCLUSIONS
The research provides experimental datas for the design of transdermal delivery system of brucine.
Chen
Jun
J
College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210029, China. chenjun75@163.com
Hu
Wei
W
Cai
Bao-Chang
BC
Hu
Min-Min
MM
D000279:Administration, Cutaneous; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D066298:In Vitro Techniques; D008297:Male; D010539:Permeability; D019999:Pharmaceutical Solutions; D010946:Plants, Medicinal / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000378:metabolism; D012869:Skin Absorption; D012995:Solubility; D013331:Strychnine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D031461:Strychnos nux-vomica / Q000737:chemistry*
D019999:Pharmaceutical Solutions; C083806:brucine; D013331:Strychnine