Stathmin-like 2, a developmentally-associated neuronal marker, is expressed and modulated during osteogenesis of human mesenchymal stem cells.
Stathmin-like 2 (STMN2) protein, a neuronal protein of the stathmin family, has been implicated in the microtubule regulatory network as a crucial element of cytoskeletal regulation. Herein, we describe that STMN2 expression increases at both mRNA and protein levels during osteogenesis of human mesenchymal stem cells derived from adipose tissue (hMADS cells) and bone marrow (hBMS cells), whereas it decreases to undetectable levels during adipogenesis. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. Thus STMN2 appears a novel marker of osteogenesis and osteoblast per se, that could play a role in the regulation of the adipocyte/osteoblast balance.
Chiellini
Chiara
C
IBDC, Université de Nice Sophia-Antipolis, CNRS, 28 Avenue Valrose, 06108 Nice cedex 2, France.
Grenningloh
Gabriele
G
Cochet
Olivia
O
Scheideler
Marcel
M
Trajanoski
Zlatko
Z
Ailhaud
Gérard
G
Dani
Christian
C
Amri
Ez-Zoubir
EZ
D017667:Adipocytes / Q000378:metabolism; D050156:Adipogenesis; D000273:Adipose Tissue / Q000378:metabolism; D000577:Amides / Q000494:pharmacology; D015415:Biomarkers / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D003600:Cytosol / Q000378:metabolism; D003907:Dexamethasone / Q000494:pharmacology; D006056:Golgi Apparatus / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000096:biosynthesis* / Q000235:genetics; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D010006:Osteoblasts / Q000187:drug effects / Q000378:metabolism; D010012:Osteogenesis; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D050777:Stathmin; D054460:rho-Associated Kinases / Q000037:antagonists & inhibitors
D000577:Amides; D015415:Biomarkers; D008565:Membrane Proteins; D047428:Protein Kinase Inhibitors; D011725:Pyridines; D012333:RNA, Messenger; C063314:STMN2 protein, human; D050777:Stathmin; C108830:Y 27632; D003907:Dexamethasone; D054460:rho-Associated Kinases