title: Modulation of fenthion-induced oxidative effects by BSO in the liver of Cyprinus carpio L.
abstract: The objective of the present study was to evaluate the oxidative stress potential of low-level organophosphate fenthion exposure with the modulatory effect of buthionine sulfoximine in the liver of Cyprinus carpio L. The fish were exposed to 20% of 96-hour LC(50) of fenthion for 24 and 96 hours. Total and oxidized glutathione, thiobarbituric acid reactive substances, protein levels, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, superoxide dismutase, and catalase-specific enzyme activities were measured spectrophotometrically. There was a 15-day depuration period to evaluate the changes in the studied parameters. Fenthion caused a time-dependent depletion of the total and reduced glutathione levels. The oxidized/reduced glutathione ratio and catalase specific enzyme activity were reduced while the glutathione-S-transferase activity was elevated. Intraperitonal buthionine sulfoximine application disclosed the inhibitory effect of fenthion on superoxide dismutase and glutathione peroxidase activities, whereas glutathione-S-transferase activity was increased. There was no change in lipid peroxidation levels during the experiments. No amelioration was observed in the affected parameters except the glutathione-S-transferase activity in the 15-day depuration period. In conclusion, glutathione-S-transferase and catalase enzyme activities and total and reduced glutathione levels were better estimators to monitor the effects of fenthion in low concentration in the liver of C. carpio. The depuration period was not adequate to recover the antioxidant capacity.
authors.affiliation: Department of Biology, University of Cukurova, Faculty of Science and Letters, Adana, Turkey. nuner@cu.edu.tr
authors.forename: Nevin
authors.initials: N
authors.lastname: Uner
authors.forename: Yusuf
authors.initials: Y
authors.lastname: Sevgiler
authors.forename: Petek
authors.initials: P
authors.lastname: Piner
authors.forename: Hülya
authors.initials: H
authors.lastname: Durmaz
mesh_terms: D000818:Animals; D019328:Buthionine Sulfoximine / Q000494:pharmacology*; D002347:Carps; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005284:Fenthion / Q000008:administration & dosage / Q000633:toxicity*; D005978:Glutathione / Q000187:drug effects / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000187:drug effects / Q000378:metabolism; D005982:Glutathione Transferase / Q000187:drug effects / Q000378:metabolism; D007306:Insecticides / Q000008:administration & dosage / Q000633:toxicity; D007928:Lethal Dose 50; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D013997:Time Factors
chemical_list: D004791:Enzyme Inhibitors; D007306:Insecticides; D019328:Buthionine Sulfoximine; D005284:Fenthion; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase; D005978:Glutathione