title: [Maturation of dendritic cells and activation of B-lymphocytes in spleens of ICR mice infected with chloroquine-resistant Plasmodium berghei].
abstract: OBJECTIVE
  To investigate the relation between activation of B-cells and maturation of dendritic cells (DC) in the spleens of ICR mice infected with chloroquine-resistant (RC) or chloroquine-sensitive (N) strain of Plasmodium berghei.
  
  
  METHODS
  Spleens were taken after the mice were infected with N or RC strains of P. berghei and attained certain degree of parasitemia. Changes of B-cells and DCs were examined by pathological method, immunohistochemistry and immunofluorescence methods, transmission electron microscopy (TEM) and flow cytometry technology.
  
  
  RESULTS
  Proliferation of white pulps in the spleen of mice infected with RC strain was found as compared to that with N strain. The percentage of cluster of differentiation (CD) 45R/B220, CD19 cells increased in the spleen cells, number of medium and small lymphocytes increased in the germinal centers, the immature and mature plasma cells also increased in the red pulps of spleen in RC strain-infected mice. On the contrary, in the N strain-infected mice spleen, the white pulps were reduced and the red pulps were filled with parasite-infected red blood cells; less small lymphocytes, immature and mature plasma cells were observed in red pulps. The number of CD11c DCs increased, especially in the periarteriolar lymphoid sheath, T cell area; the expression of major histocompatibility complex II (MHC II) on DC was up-regulated in RC strain-infected mice as compared to that in N strain-infected mice. TEM showed that the DCs in RC strain-infected mice spleens were more active than that in N strain-infected mice.
  
  
  CONCLUSIONS
  Infection of RC strain P. berghei increases mature DCs in the spleen, which induces the proliferation of B cells and immune response.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 201101, China.
authors.forename: Ke-Qiang
authors.initials: KQ
authors.lastname: Chen
authors.forename: Huai-Min
authors.initials: HM
authors.lastname: Zhu
authors.forename: Can-Rong
authors.initials: CR
authors.lastname: Ni
authors.forename: Guan-Hong
authors.initials: GH
authors.lastname: Song
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000166:cytology / Q000276:immunology*; D002738:Chloroquine / Q000494:pharmacology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology*; D004351:Drug Resistance; D006790:Host-Parasite Interactions; D008288:Malaria / Q000276:immunology / Q000469:parasitology*; D051379:Mice; D008813:Mice, Inbred ICR; D010962:Plasmodium berghei / Q000187:drug effects / Q000502:physiology*; D013154:Spleen / Q000166:cytology / Q000276:immunology
chemical_list: D002738:Chloroquine