[Establishing and identifying insulin resistance 3T3-L1 adipocytes cell model].
OBJECTIVE
To establish insulin-resistant 3T3-L1 adipocytes cell model.


METHODS
3T3-L1 preadipocytes were cultured and induced to differentation. We established insulin resistant 3T3-L1 induced by dexamethasone and determined the change of glucose concentration in cell culture.


RESULTS
In high glucose DMEM culture media, the glusose concentration significantly decreased. Achieved the peak of insulin-resisitant at the 96th hour induced by dexamethasone, and at this time compared with the blank the mRNA expression of Resistin of the model step up about three times.


CONCLUSIONS
3T3-L1 adipocyte exposed to 1 micromol/L dexamethasone for 24 hours are able to induce a state of insulin resistance which can be maintained for 216 hours.
Guo
Xiao-Nong
XN
Life Science and Engineering Institute of Northwest University for Nationalities, Lanzhou 730030, China. gxnwww@126.com
Yang
Ju-Tian
JT
Niu
Feng
F
Zhang
Ru-Xue
RX
Jia
Zheng-Ping
ZP
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D007333:Insulin Resistance; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D052243:Resistin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D003470:Culture Media; D012333:RNA, Messenger; D052243:Resistin; D003907:Dexamethasone; D005947:Glucose