Heat shock protein 60 induces inflammatory mediators in mouse adipocytes.
Adipocytes represent an important cellular source of inflammatory mediators. However, the signals for the induction of proinflammatory adipocyte activities are largely unknown. Here, we demonstrate that heat shock protein (Hsp) 60, a potent stimulator of innate immunity, induces the release of the inflammatory mediators interleukin-6, CXCL1 and monocyte chemoattractant protein-1 in a time- and concentration-dependent manner from cells of the adipocyte line 3T3-L1 and from adipocytes of obese mice. These results identify Hsp60 as an important regulator of adipocyte functions which contribute to the development of inflammatory processes as observed in diabetes and diabetes-associated complications.
Gülden
Elke
E
German Diabetes Center, Institute for Clinical Diabetes Research, Auf'm Hennekamp 65, D-40225 Düsseldorf, Germany.
Mollérus
Sina
S
Brüggemann
Jutta
J
Burkart
Volker
V
Habich
Christiane
C
D016475:3T3 Cells; D017667:Adipocytes / Q000187:drug effects / Q000276:immunology*; D000818:Animals; D018834:Chaperonin 60 / Q000494:pharmacology / Q000502:physiology*; D018932:Chemokine CCL2 / Q000378:metabolism; D054360:Chemokine CXCL1 / Q000378:metabolism; D018836:Inflammation Mediators / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology; D051379:Mice; D008815:Mice, Inbred Strains
D018834:Chaperonin 60; D018932:Chemokine CCL2; D054360:Chemokine CXCL1; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides