title: [Study on technical processes for extraction and purification of total tanshinones].
abstract: OBJECTIVE
  To optimize technical processes for extraction and purification of total tanshinones from Radix Salviae Miltiorrhizae.
  
  
  METHODS
  Using orthogonal design, and taking the extraction percentage of total tanshinones and tanshinone IIA as indexes, the extraction conditions of total tanshinones from Radix Salviae Miltiorrhizae and the purification conditions of total tanshinones from crude extract were optimized respectively.
  
  
  RESULTS
  The optimal technical process for extraction of total tanshinones was as follows: added 7 volumes of 90% aqueous ethanol, extracted three times and each time for 1.5 hours. The optimal technical process for purification of total tanshinones was as follows: 15 volumes of 8% sodium carbonate solution, washing 5 times and each time stirring for 5 minutes.
  
  
  CONCLUSIONS
  The content of total tanshinones in crude extract increased from 18.23% to 57.29% by purification. The optimal technical processes for extraction and purification of total tanshinones are practical in industrial production.
authors.affiliation: Department of Pharmaceutical Engineering, Hefei University of Technology, Hefei 230009, China. zxxwyfzsy@sina.com
authors.forename: Xiao-Xiang
authors.initials: XX
authors.lastname: Zhang
authors.forename: Shao-Bing
authors.initials: SB
authors.lastname: Chen
authors.forename: Yan-Ping
authors.initials: YP
authors.lastname: Wang
mesh_terms: D045784:Abietanes; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D010616:Phenanthrenes / Q000032:analysis / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
chemical_list: D045784:Abietanes; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D012997:Solvents; C021751:tanshinone; D000431:Ethanol