title: The amylase-secreting cells of the stomach of the scallop, Pecten maximus: ultrastructural, immunohistochemical and immunocytochemical characterizations.
abstract: (1) alpha-amylase was extracted and purified from the stomach/digestive gland complex of the scallop Pecten maximus and an anti-serum was induced against the purified amylase by rabbit immunization. (2) The anti scallop amylase was used to localize the amylase-secreting cells in the stomach of Pecten maximus by immunofluorescence and immunogold labelling. The amylase-secreting cells are glandular cells particularly numerous in the main sorting area of the stomach. Their secretory granules were found strongly positive for anti-amylase. Three types of glandular cells were observed, actually corresponding to the three stages of the glandular-cell activity, synthesis, secretion and excretion. (3) The synthesizing cell shows the characteristic features of a protein-synthesizing cell: a conspicuous nucleolus and abundant granular endoplasmic reticulum. In the secretory cell, the secretory granules are formed by the Golgi apparatus and accumulate in the apical part of the cell. The secretory cell is filled with two types of secretory granules which are released in the stomach lumen by apocrine excretion. (4) The present study brings the first demonstration of the synthesis and extracellular release of amylase by glandular cells of the stomach epithelium of a bivalve.
authors.affiliation: Département de Physiologie & Neurophysiologie, Faculté St. Jérôme, CP 351-352, 13397, Marseille Cedex 20, France.
authors.forename: M
authors.initials: M
authors.lastname: Henry
authors.forename: N
authors.initials: N
authors.lastname: Benlimame
authors.forename: E
authors.initials: E
authors.lastname: Boucaud-Camou
authors.forename: M
authors.initials: M
authors.lastname: Mathieu
authors.forename: A
authors.initials: A
authors.lastname: Donval
authors.forename: A
authors.initials: A
authors.lastname: Van Wormhoudt