Application of the iodine-azide postcolumn reaction in RP-HPLC for the determination of thioguanine in urine.
The study focuses on the analysis of a sensitive, selective, and simple postcolumn detection method for thioguanine determination, based on the sensitizing induction of thioguanine on iodine-azide reaction and the combination technique of HPLC. The analysis was accomplished in the optimum conditions for iodine-azide detection system and HPLC separation. The values for the linear range, the LOD, and DOQ amounted to 0.8-1.7, 0.4, and 0.5 nmol/mL urine, respectively.
Zakrzewski
Robert
R
Department of Instrumental Analysis, University of Łódź, Pomorska 163, 90-236 Łódź, Poland. robzak@chemul.uni.lodz.pl
D000964:Antimetabolites, Antineoplastic / Q000652:urine; D001386:Azides; D002851:Chromatography, High Pressure Liquid / Q000379:methods* / Q000706:statistics & numerical data; D016903:Drug Monitoring / Q000379:methods; D006801:Humans; D007455:Iodine; D012680:Sensitivity and Specificity; D013866:Thioguanine / Q000652:urine*
D000964:Antimetabolites, Antineoplastic; D001386:Azides; D007455:Iodine; D013866:Thioguanine