title: Acanthamoeba spp. cysts storage in filter paper.
abstract: A new method of storage of the free-living amoeba, Acanthamoeba, is described. For this purpose, strips of filter paper were impregnated with cysts of amoebas cultivated axenically and transferred into a cryotube. The strips were dehydrated at 30 degrees C for 24 h and after storage at room temperature. The amoeba viability has been evaluated and the cysts remain viable and pure for at least 1 year. Then, our method has been efficient for the storage of cysts during the evaluated period.
authors.affiliation: Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil. claitonjp@hotmail.com
authors.forename: Claiton José
authors.initials: CJ
authors.lastname: Pens
authors.forename: Marilise Brittes
authors.initials: MB
authors.lastname: Rott
mesh_terms: D000048:Acanthamoeba / Q000502:physiology*; D000818:Animals; D010209:Paper; D011309:Preservation, Biological; D013997:Time Factors