title: Rapid culture-based methods for drug-resistance detection in Mycobacterium tuberculosis.
abstract: Tuberculosis still represents a major public health problem, especially in low-resource countries where the burden of the disease is more important. Multidrug-resistant and extensively drug drug-resistant tuberculosis constitute serious problems for the efficient control of the disease stressing the need to investigate resistance to first- and second-line drugs. Conventional methods for detecting drug-resistance in Mycobacterium tuberculosis are slow and cumbersome. The most commonly used proportion method on Löwenstein-Jensen medium or Middlebrook agar requires a minimum of 3-4 weeks to produce results. Several new approaches have been proposed in the last years for the rapid and timely detection of drug-resistance in tuberculosis. This review will address phenotypic culture-based methods for rapid drug susceptibility testing in M. tuberculosis.
authors.affiliation: Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerp, Belgium. jcpalomino@itg.be
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Palomino
authors.forename: Anandi
authors.initials: A
authors.lastname: Martin
authors.forename: Andrea
authors.initials: A
authors.lastname: Von Groll
authors.forename: Francoise
authors.initials: F
authors.lastname: Portaels
mesh_terms: D000995:Antitubercular Agents / Q000494:pharmacology*; D003470:Culture Media; D024881:Drug Resistance, Bacterial; D006801:Humans; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009169:Mycobacterium tuberculosis / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification; D010641:Phenotype; D013997:Time Factors; D014376:Tuberculosis / Q000382:microbiology
chemical_list: D000995:Antitubercular Agents; D003470:Culture Media