3'-Terminal nucleotide sequence (N = 41) of satellite tobacco necrosis virus RNA.
To convert satellite tobacco necrosis virus RNA into a template for complementary DNA (cDNA) synthesis, a poly(A) tail was added to its 3'-end by means of Escherichia coli ATP:RNA adenyltransferase. This product could then be copied by avian myeloblastosis virus RNA-dependent DNA polymerase in the presence of p(dT)(10) as primer. Under appropriate conditions, cDNA was synthesized discontinuously, and limited transcripts were obtained when one or more deoxynucleoside triphosphates were omitted from the reaction mixture. "Phased" cDNA fragments suitable for sequence analysis were synthesized when (dT)(8)dG, (32)P-labeled at the 5'-end, was used as a primer. We report here the sequence of the 3'-terminal 41 nucleotides of the viral RNA.
Merregaert
J
J
Laboratory of Molecular Biology, State University of Ghent, Ledeganckstraat, 35, B-9000 Ghent, Belgium.
Emmelo
J V
JV
Fiers
W
W