title: Analytical investigation: assay of D-lactate in diabetic plasma and urine.
abstract: OBJECTIVE
  In this study we developed a 96-micro plate enzymatic assay for D-lactate in plasma and urine.
  
  
  METHODS
  D-lactate was assayed enzymatically with a UV-spectrophotometer in plasma from 38 and in urine from 37 diabetics and from 42 non-diabetic controls.
  
  
  RESULTS
  The within-run coefficients of variations (CV) were 2.6% for plasma and 5.7% for urine. The between run CVs were 6.8% for plasma and 6.7% for urine. The mean recovery with standard deviation (S.D.) was 107.4+/-7.3% for plasma and 100.1%+/-6.7% for urine. The plasma D-lactate in diabetics were (mean+/-S.D.) 39.6+/-23.7 microM. We found significant difference between the urinary d-lactate in controls and diabetics (18.2+/-12.0 vs. 35.9+/-24.2 microM/mM creatinine, p<0.0001).
  
  
  CONCLUSIONS
  The assay proved reliable with acceptable precision and recovery. Results suggest that diabetics have elevated urinary and plasma D-lactate as compared to controls.
authors.affiliation: University of Kuopio, Finland.
authors.forename: J Petteri
authors.initials: JP
authors.lastname: Talasniemi
authors.forename: Sirpa
authors.initials: S
authors.lastname: Pennanen
authors.forename: Heikki
authors.initials: H
authors.lastname: Savolainen
authors.forename: Leo
authors.initials: L
authors.lastname: Niskanen
authors.forename: Jyrki
authors.initials: J
authors.lastname: Liesivuori
mesh_terms: D000328:Adult; D000368:Aged; D003920:Diabetes Mellitus / Q000097:blood* / Q000652:urine*; D005260:Female; D006801:Humans; D019344:Lactic Acid / Q000097:blood* / Q000652:urine*; D008297:Male; D008875:Middle Aged; D013237:Stereoisomerism
chemical_list: D019344:Lactic Acid