Genotypic change of porcine circovirus type 2 on Japanese pig farms as revealed by restriction fragment length polymorphism analysis.
Porcine circovirus type 2 (PCV2) has been recognized as the causal agent of postweaning multisystemic wasting syndrome and can be divided into two major genotypic groups. We developed a method of restriction fragment length polymorphism (RFLP) analysis of PCV2 open reading frame 2 for easy discrimination between the two major groups. Genotyping of PCV2 isolates from 10 Japanese commercial pig farms was performed, and the analysis revealed that both PCV2 groups and at least five RFLP types of PCV2 are prevalent in Japan. On two farms, the genotypes of the PCV2 isolates in the spring of 2007 were different from those in the autumn of 2006. One genotype may have become dominant within only six months on these farms.
Takahagi
Yoichi
Y
Research and Development Center, Nippon Meat Packers, Inc., Ibaraki, Japan. y.takahagi@nipponham.co.jp
Nishiyama
Yasutaka
Y
Toki
Shinji
S
Yonekita
Taro
T
Morimatsu
Fumiki
F
Murakami
Hiroshi
H
D000818:Animals; D001483:Base Sequence; D018173:Circoviridae Infections / Q000453:epidemiology / Q000235:genetics / Q000662:veterinary*; D017925:Circovirus / Q000235:genetics*; D016000:Cluster Analysis; D017931:DNA Primers / Q000235:genetics; D005838:Genotype; D007564:Japan / Q000453:epidemiology; D008969:Molecular Sequence Data; D010802:Phylogeny; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D017422:Sequence Analysis, DNA; D034421:Sus scrofa; D013553:Swine Diseases / Q000453:epidemiology* / Q000235:genetics*
D017931:DNA Primers