Incongruence in the pattern and timing of intra-specific diversification in bronze frogs and bullfrogs (Ranidae).
We compare patterns of lineage divergence in mitochondrial DNA (mtDNA) sequences of two protein-encoding mitochondrial genes (cyt b and ND2) in two ecologically similar, co-distributed, and closely related ranid frogs (Rana clamitans and Rana catesbeiana), that are geographically widespread, and frequently syntopic. We identified three lineages in R. clamitans, separated by 0.5% to 2.1% net corrected sequence divergence, comparable to two R. catesbeiana lineages separated by 0.6%. The geographic pattern of lineage distribution differed notably between the two species. In R. clamitans, we found a Coastal Plain-Appalachian (CPA) lineage restricted to south and east of the Appalachian Mountains and a widespread lineage that encompassing nearly all the sampled range. A third distinct and divergent lineage was detected in one location in the southwest portion of the range (Louisiana). This pattern contrasts with the east-west pattern in R. catesbeiana, and reflects possible differences in refugial dynamics and patterns of range expansion. Although both species have undergone range expansion and population growth, coalescent reconstruction of N(e) reflects larger lineages but more recent divergence in R. clamitans relative to R. catesbeiana, reflecting significant differences in population history or divergent patterns of molecular evolution at mtDNA.
Austin
James D
JD
Wildlife Ecology & Conservation, 110 Newins-Ziegler Hall, P.O. Box 110430, University of Florida, Gainesville, Florida 32611, USA. austinl@ufl.edu
Zamudio
Kelly R
KR
D000818:Animals; D005075:Biological Evolution; D019070:Cell Lineage; D045303:Cytochromes b / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics*; D019143:Evolution, Molecular; D014644:Genetic Variation; D005843:Geography; D006239:Haplotypes; D007438:Introns; D008957:Models, Genetic; D010802:Phylogeny; D011898:Ranidae / Q000235:genetics*; D017422:Sequence Analysis, DNA; D013045:Species Specificity
D004272:DNA, Mitochondrial; D045303:Cytochromes b