Correlation between DJ-1 levels in the cerebrospinal fluid and the progression of disabilities in multiple sclerosis patients.
OBJECTIVE
DJ-1 plays a key role in the anti-oxidative stress function. Increasing evidence supports the role of oxidative stress in the pathogenesis of multiple sclerosis (MS). The aim of this study was to investigate whether the DJ-1 levels were increased in patients with MS and to examine its association with the progression of MS.


METHODS
Quantitative immunoblot assays were performed to evaluate the DJ-1 level in serum and cerebrospinal fluid (CSF) collected from relapsing-remitting patients with MS (n = 29), disease controls subjects (n = 14), and healthy subjects (n = 44).


RESULTS
No significant difference was observed in the serum DJ-1 level among the patients with MS, disease controls, and healthy controls. However, the CSF DJ-1 levels were significantly higher in the patients with MS than in the disease control subjects (P < 0.0001). A significant positive correlation was also found between the CSF DJ-1 levels and the Multiple Sclerosis Severity Score (P < 0.005, r = 0.501).


CONCLUSIONS
These results show that the CSF DJ-1 levels are significantly increased in the CSF of patients with MS and that the CSF DJ-1 levels may be associated with the disease progression of MS. Therefore, DJ-1 possibly plays an important role in the pathogenesis of MS.
Hirotani
M
M
Department of Neurology, Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
Maita
C
C
Niino
M
M
Iguchi-Ariga
Sm
S
Hamada
S
S
Ariga
H
H
Sasaki
H
H
D000328:Adult; D006233:Persons with Disabilities; D018450:Disease Progression; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000097:blood / Q000134:cerebrospinal fluid*; D008297:Male; D009103:Multiple Sclerosis / Q000097:blood / Q000134:cerebrospinal fluid / Q000503:physiopathology*; D015513:Oncogene Proteins / Q000097:blood / Q000134:cerebrospinal fluid*; D000071617:Protein Deglycase DJ-1; D012016:Reference Values; D012720:Severity of Illness Index
D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1