[Improvement of synthesis of quercetin and genistein sulfates and identification by HPLC-MS].
OBJECTIVE
To improve synthesis of quercetin and genistein sulfates.


METHODS
Quercetin and genistein were esterified with concentrated sulfuric acid for 3 hours in ice and esterfication was terminated by neutralization with 6N NaOH. Derivatives were identified by HPLC-APCI-MS.


RESULTS
Quercetin and genistein derivatives were only one compound of monosulfate.


CONCLUSIONS
The improvement of synthesis of quercetin and genistein sulfates is a convenient method for obtaining soluble derivatives of the flavonoids.
Liu
Wen
W
Guangdong Medical College, Institute of Biochemistry and Molecular Biology, Zhanjiang 524023, China.
Deng
Yi-Feng
YF
Liang
Nian-Ci
NC
D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004951:Esterification; D019833:Genistein / Q000737:chemistry*; D013058:Mass Spectrometry / Q000379:methods*; D011794:Quercetin / Q000737:chemistry*; D013463:Sulfuric Acid Esters / Q000032:analysis / Q000138:chemical synthesis* / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods; D014867:Water / Q000737:chemistry
D013463:Sulfuric Acid Esters; D014867:Water; D011794:Quercetin; D019833:Genistein