Supplementing vitamin B6 to a low vitamin B6 diet exaggerates UVB-induced skin tumorigenesis in DMBA-treated hairless mice.
7,12-Dimethylbenz[a]anthracene (DMBA)-treated hairless mice exposed to UVB radiation were used to examine the effect of graded levels of vitamin B(6) [1, 7 or 35 mg pyridoxine (PN) HCl/kg] on skin tumorigenesis for 18 wk. Compared to the 1 mg PN HCl/kg diet, the 35 mg PN HCl/kg diet significantly elevated the incidence and multiplicity of skin tumors, while there was no difference in skin tumorigenesis between the 7 and 35 mg PN HCl/kg diets. Skin levels of oxidative stress markers (lipid peroxides and protein carbonyls) were unaffected by dietary treatment. Compared to the 1 mg PN HCl/kg diet, the 7 and 35 mg PN HCl/kg diets significantly elevated serum pyridoxal 5'-phosphate (PLP) without affecting the skin level of PLP. The results suggest that dietary supplemental vitamin B(6) exaggerates UVB-induced skin tumorigenesis in hairless mice without affecting oxidative stress in the skin.
Lu
Tao
T
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Japan.
Xu
Yonghui
Y
Monttinen
Elise Saiz
ES
Kato
Norihisa
N
D015127:9,10-Dimethyl-1,2-benzanthracene / Q000008:administration & dosage*; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002273:Carcinogens / Q000008:administration & dosage; D004032:Diet / Q000009:adverse effects / Q000379:methods; D019587:Dietary Supplements / Q000009:adverse effects*; D004305:Dose-Response Relationship, Drug; D005260:Female; D051379:Mice; D008812:Mice, Hairless; D018384:Oxidative Stress / Q000187:drug effects / Q000528:radiation effects; D011732:Pyridoxal Phosphate / Q000097:blood; D011736:Pyridoxine / Q000378:metabolism / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D012878:Skin Neoplasms / Q000097:blood / Q000378:metabolism*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014466:Ultraviolet Rays / Q000009:adverse effects*; D025101:Vitamin B 6 / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology*; D026681:Vitamin B 6 Deficiency / Q000188:drug therapy / Q000378:metabolism*; D014803:Vitamin B Complex / Q000009:adverse effects / Q000378:metabolism / Q000494:pharmacology
D015415:Biomarkers; D002273:Carcinogens; D017392:Thiobarbituric Acid Reactive Substances; D014803:Vitamin B Complex; D015127:9,10-Dimethyl-1,2-benzanthracene; D011732:Pyridoxal Phosphate; D025101:Vitamin B 6; D011736:Pyridoxine