Degradation of liposome cluster caused by the interaction with endocrine disrupting chemicals (EDCs).
The interaction between endocrine disrupting chemicals (EDCs) and liposome clusters was investigated using UV-vis spectroscopy and observed by microscope. Since liposome clusters are composed of small unilamellar liposomes and membrane proteins, they are regarded as a model of plasma membranes. The size of each cluster was 10microm in diameter. UV-vis spectroscopy of liposome clusters showed an apparent absorption at 300nm which was affected by the interaction with EDCs. Four EDCs caused an alteration in absorption in a concentration-dependence manner between 0.1 and 10ppm (i.e. 0.1-10mg/l). Microscopic observation showed that the effect was caused by the degradation of liposome clusters while degradation was induced by the interaction; liposome was broken down by EDCs. This report suggests that the liposome cluster can be applied for universal detection of EDCs based on the interaction between a plasma membrane model and EDCs.
Nakane
Yuko
Y
Faculty of Engineering, Soka University, 1-236 Tangi-cho, Hachioji, Tokyo 192-8577, Japan.
Kubo
Izumi
I
D002462:Cell Membrane / Q000378:metabolism; D004347:Drug Interactions; D052244:Endocrine Disruptors / Q000737:chemistry* / Q000378:metabolism*; D005453:Fluorescence; D008081:Liposomes / Q000737:chemistry* / Q000378:metabolism*; D013056:Spectrophotometry, Ultraviolet
D052244:Endocrine Disruptors; D008081:Liposomes