Size determination of Orgyia pseudotsugata cytoplasmic polyhedrosis virus genomic RNA.
Molecular-weight estimates were determined for the Orgyia pseudotsugata cytoplasmic polyhedrosis virus (CPV) genome. The molecular-weight estimates were obtained from measurements of glyoxal denatured RNA molecules following electrophoresis in 1% agarose gels and electron micrographic contour lengths. These estimates were compared to the minimal coding capacity derived from the size of translation products of selected genomic segments. The results obtained from these different experimental approaches were in sufficient agreement to estimate the genome size at 20.4 x 106 (29.8 kbp) with a segmental range from 0.61 to 3.53 x 10(6) (0.89-5.19 kbp). Two other members of the Reoviridae family were examined, the Bombyx mori CPV and the human reovirus type 3 (Dearing strain). The molecular-weight estimates for these two viruses were 20.3 and 19.1 x 10(6), respectively. These results suggest that the previously published molecular-weight estimates for these various double-stranded RNA genomes were underestimated.
Galinski
M S
MS
Department of Agricultural Chemistry Oregon State University, Corvallis, Oregon 97331, USA.
Chow
K C
KC
Rohrmann
G F
GF
Pearson
G D
GD
Beaudreau
G S
GS