title: An Efficient reproductive method for Irs2-/- mice with C57BL/6JJcl genetic background.
abstract: Efficient reproduction using natural mating and reproduction technology [in vitro fertilization (IVF) and embryo transfer (ET)] was investigated in IRS2 deficient mice with C57BL/6JJcl genetic background (Irs2(-/-) mice) as a typical type 2 diabetes model. From the results using various combinations of Irs2(-/-) and Irs2(-/+) mice, the combination of female Irs2(-/+) x male Irs2(-/-) was found to be more efficient than other combinations. In applications of reproduction technology using IVF and ET, the combination of female Irs2(-/+) x male Irs2(-/-) involves the possibility of Irs2(-/-) production by repeats using female Irs2(-/+) mice. However, reproductive continuity using this combination is difficult because of dependence on human technique and the cost of ET. Therefore, we concluded that Irs2(-/-) mice should be produced by embryo transfer using Irs2(-/-) mice from a colony consisting of female Irs2(-/+) x male Irs2(-/-).
authors.affiliation: Central Institute for Experimental Animals, Kanagawa, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Hashimoto
authors.forename: Tomoo
authors.initials: T
authors.lastname: Eto
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kamisako
authors.forename: Naoko
authors.initials: N
authors.lastname: Hoya
authors.forename: Teruhiko
authors.initials: T
authors.lastname: Hatakeyama
authors.forename: Toshiro
authors.initials: T
authors.lastname: Arai
authors.forename: Makoto
authors.initials: M
authors.lastname: Yokosuka
authors.forename: Yasuyuki
authors.initials: Y
authors.lastname: Ohnishi
authors.forename: Mamoru
authors.initials: M
authors.lastname: Ito
authors.forename: Kyoji
authors.initials: K
authors.lastname: Hioki
authors.forename: Ryo
authors.initials: R
authors.lastname: Suzuki
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Ohsugi
authors.forename: Muneo
authors.initials: M
authors.lastname: Saito
authors.forename: Yoshito
authors.initials: Y
authors.lastname: Ueyama
authors.forename: Toshimasa
authors.initials: T
authors.lastname: Yamauchi
authors.forename: Naoto
authors.initials: N
authors.lastname: Kubota
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Tobe
authors.forename: Takashi
authors.initials: T
authors.lastname: Kadowaki
authors.forename: Norikazu
authors.initials: N
authors.lastname: Tamaoki
authors.forename: Tatsuji
authors.initials: T
authors.lastname: Nomura
authors.forename: Kinori
authors.initials: K
authors.lastname: Kosaka
mesh_terms: D000818:Animals; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics; D004624:Embryo Transfer / Q000379:methods*; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL / Q000235:genetics*