Circulating endothelial progenitor cells as a pathogenetic marker of moyamoya disease.
Moyamoya disease (MMD) is an unusual form of chronic cerebrovascular occlusive disease that involves the formation of characteristically abnormal vessels. Recent studies have reported that colony-forming unit (CFU) and outgrowth cells represent a subpopulation of endothelial progenitor cells (EPCs). Here, we attempted to determine the significance of CFU number and outgrowth cell yield in MMD. Endothelial progenitor cells were isolated from the blood of 24 adult MMD patients and from 48 age- and risk factor-matched control subjects. After 7 days of culture, CFUs were determined, and yields of outgrowth cells were measured during 2 months of culture. The EPC function was also evaluated using matrigel plate assays. It was found that CFU numbers were significantly lower in MMD patients than in controls. Moreover, during long-term culture, outgrowth cells were isolated from only 10% of control subjects but from 33% of MMD patients, and CFU numbers and tube formation were found to be lower in advanced MMD cases than in those with early stage disease, whereas outgrowth cells were more frequently detected in those with early MMD and moyamoya vessels than in those with advanced disease. These characteristics of circulating EPCs reflect mixed conditions of vascular occlusion and abnormal vasculogenesis during the pathogenesis of MMD.
Jung
Keun-Hwa
KH
Stroke and Stem Cell Laboratory, Department of Neurology, Clinical Research Institute, Seoul National University Hospital, Seoul, South Korea.
Chu
Kon
K
Lee
Soon-Tae
ST
Park
Hee-Kwon
HK
Kim
Dong-Hyun
DH
Kim
Jin-Hee
JH
Bahn
Jae-Jun
JJ
Song
Eun-Cheol
EC
Kim
Manho
M
Lee
Sang Kun
SK
Roh
Jae-Kyu
JK
D000328:Adult; D000714:Anastomosis, Surgical; D015415:Biomarkers / Q000097:blood*; D002533:Cerebral Angiography; D002536:Cerebral Arteries / Q000601:surgery; D002543:Cerebral Hemorrhage / Q000453:epidemiology; D003094:Collagen; D003114:Colony-Forming Units Assay; D004338:Drug Combinations; D005260:Female; D006801:Humans; D002546:Ischemic Attack, Transient / Q000453:epidemiology; D007797:Laminin; D007963:Leukocytes, Mononuclear / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009072:Moyamoya Disease / Q000097:blood* / Q000145:classification / Q000000981:diagnostic imaging / Q000473:pathology; D011509:Proteoglycans; D012016:Reference Values; D012307:Risk Factors; D020521:Stroke / Q000145:classification / Q000453:epidemiology; D013699:Temporal Arteries / Q000601:surgery
D015415:Biomarkers; D004338:Drug Combinations; D007797:Laminin; D011509:Proteoglycans; C059485:matrigel; D003094:Collagen