title: Imaging liver metastases of colorectal cancer patients with radiolabelled bevacizumab: Lack of correlation with VEGF-A expression.
abstract: OBJECTIVE
  To investigate the correlation between tumour accumulation of In-111-bevacizumab and VEGF-A expression in patients with colorectal liver metastases.
  
  
  METHODS
  Two weeks before resection of the liver metastases 12 patients were intravenously injected with In-111-labelled bevacizumab. Ten minutes and 7 d after injection a whole body scan was acquired. Seven days after the injection, 3D acquisition SPECT of the liver was performed.
  
  
  RESULTS
  Enhanced uptake of In-111-bevacizumab in the liver metastases was observed in 9 of the 12 patients. The level of antibody accumulation in these lesions varied considerably. There was no correlation between the level of In-111-antibody accumulation and the level of VEGF-A expression in the tissue as determined by in situ hybridisation and ELISA.
  
  
  CONCLUSIONS
  In this study, we investigated the correlation between tumour accumulation of radiolabelled bevacizumab and VEGF-A expression in patients with colorectal liver metastases. No clear-cut correlation between the level of antibody accumulation and expression of VEGF-A was found.
authors.affiliation: Department of Surgery, Radboud University Nijmegen Medical Centre, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands. M.Scheer@chir.umcn.nl
authors.forename: Marian G W
authors.initials: MG
authors.lastname: Scheer
authors.forename: Thamar H
authors.initials: TH
authors.lastname: Stollman
authors.forename: Otto C
authors.initials: OC
authors.lastname: Boerman
authors.forename: Kiek
authors.initials: K
authors.lastname: Verrijp
authors.forename: Fred C G J
authors.initials: FC
authors.lastname: Sweep
authors.forename: William P J
authors.initials: WP
authors.lastname: Leenders
authors.forename: Theo J M
authors.initials: TJ
authors.lastname: Ruers
authors.forename: Wim J G
authors.initials: WJ
authors.lastname: Oyen
mesh_terms: D000368:Aged; D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D000068258:Bevacizumab; D015179:Colorectal Neoplasms; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D007205:Indium Radioisotopes; D008113:Liver Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism / Q000556:secondary*; D008297:Male; D008875:Middle Aged; D016719:Radioimmunodetection; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D061067:Antibodies, Monoclonal, Humanized; D007205:Indium Radioisotopes; D042461:Vascular Endothelial Growth Factor A; D000068258:Bevacizumab