Studies on a temperature-sensitive step essential to herpesvirus DNA replication.
The nature of a previously described (Stevens, 1966) heat-sensitive step essential to the replication of infectious bovine rhinotracheitis virus deoxyribonucleic acid has been investigated. By temperature "shift down" experiments, the step was found to occur 1 hour after infection. In addition, the virus was shown to induce increased activity of a unique DNA polymerase at both 37 degrees and 42 degrees. These results, when coupled with previous data, suggest that the heat-sensitive step is concerned with an essential change either in the intracellular location or in the physical state of the infecting DNA molecule.
Stevens
J G
JG
UCLA School of Medicine, Department of Medical Microbiology and Immunology, Los Angeles, California 90024, USA.
Jackson
N L
NL