title: Specific N-terminal protein labelling: use of FMDV 3C pro protease and native chemical ligation.
abstract: We report an effective strategy for generating N-terminal cysteinyl proteins by proteolytic cleavage using the enzyme 3C pro, suitable for a wide range of applications via native chemical ligation.
authors.affiliation: Department of Chemistry and the Chemical Biology Centre, Imperial College London, South Kensington Campus, London, UK.
authors.forename: Gillian K
authors.initials: GK
authors.lastname: Busch
authors.forename: Edward W
authors.initials: EW
authors.lastname: Tate
authors.forename: Piers R J
authors.initials: PR
authors.lastname: Gaffney
authors.forename: Erika
authors.initials: E
authors.lastname: Rosivatz
authors.forename: Rudiger
authors.initials: R
authors.lastname: Woscholski
authors.forename: Robin J
authors.initials: RJ
authors.lastname: Leatherbarrow
mesh_terms: D000086042:3C Viral Proteases; D000595:Amino Acid Sequence; D002851:Chromatography, High Pressure Liquid; D003545:Cysteine / Q000737:chemistry*; D003546:Cysteine Endopeptidases / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D029544:Foot-and-Mouth Disease Virus / Q000201:enzymology; D017434:Protein Structure, Tertiary; D011506:Proteins / Q000378:metabolism*; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: D011506:Proteins; D011993:Recombinant Fusion Proteins; D014764:Viral Proteins; D003546:Cysteine Endopeptidases; D000086042:3C Viral Proteases; D003545:Cysteine