title: Capillary electrophoresis of some free fatty acids using partially aqueous electrolyte systems and indirect UV detection. Application to the analysis of oleic and linoleic acids in peanut breeding lines.
abstract: This study has shown for the first time the suitability of CE with a partially aqueous electrolyte system for the analysis of free fatty acids (FFAs) in small portions of single peanut seeds. The partially aqueous electrolyte system consisted of 40 mM Tris, 2.5 mM adenosine-5'-monophosphate (AMP) and 7 mM alpha-CD in (N-methylformamide) NMF/dioxane/water (5:3:2 by volume) mixture, pH 8-9. While AMP served as the background UV absorber for indirect UV detection of the FFAs, the alpha-CD functioned as the selectivity modulator by affecting the relative effective electrophoretic mobilities of the various FFAs due to their differential association with alpha-CD. This CE method allowed the screening of peanut seeds for their content of oleic and linoleic acids, which is essential in breeding of peanuts of high-oleic acid content. The extraction method of FFAs from peanut seeds is very reproducible with a high recovery approaching quantitative yield (approximately 97% recovery).
authors.affiliation: Department of Chemistry, Oklahoma State University, Stillwater, OK 74078-3071, USA.
authors.forename: Yogita C
authors.initials: YC
authors.lastname: Bannore
authors.forename: Kelly D
authors.initials: KD
authors.lastname: Chenault
authors.forename: Hassan A
authors.initials: HA
authors.lastname: Melouk
authors.forename: Ziad
authors.initials: Z
authors.lastname: El Rassi
mesh_terms: D004573:Electrolytes / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods; D008041:Linoleic Acids / Q000032:analysis*; D009829:Oleic Acids / Q000032:analysis*; D000074241:Peanut Oil; D010938:Plant Oils / Q000737:chemistry*; D015203:Reproducibility of Results; D012639:Seeds / Q000737:chemistry*; D012680:Sensitivity and Specificity; D012996:Solutions / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013997:Time Factors; D014867:Water / Q000737:chemistry
chemical_list: D004573:Electrolytes; D008041:Linoleic Acids; D009829:Oleic Acids; D000074241:Peanut Oil; D010938:Plant Oils; D012996:Solutions; D014867:Water