title: A reservoir of brown adipocyte progenitors in human skeletal muscle.
abstract: Brown adipose tissue uncoupling protein-1 (UCP1) plays a major role in the control of energy balance in rodents. It has long been thought, however, that there is no physiologically relevant UCP1 expression in adult humans. In this study we show, using an original approach consisting of sorting cells from various tissues and differentiating them in an adipogenic medium, that a stationary population of skeletal muscle cells expressing the CD34 surface protein can differentiate in vitro into genuine brown adipocytes with a high level of UCP1 expression and uncoupled respiration. These cells can be expanded in culture, and their UCP1 mRNA expression is strongly increased by cell-permeating cAMP derivatives and a peroxisome-proliferator-activated receptor-gamma (PPARgamma) agonist. Furthermore, UCP1 mRNA was detected in the skeletal muscle of adult humans, and its expression was increased in vivo by PPARgamma agonist treatment. All the studies concerning UCP1 expression in adult humans have until now been focused on the white adipose tissue. Here we show for the first time the existence in human skeletal muscle and the prospective isolation of progenitor cells with a high potential for UCP1 expression. The discovery of this reservoir generates a new hope of treating obesity by acting on energy dissipation.
authors.affiliation: Stem Cell Research Center, Children's Hospital, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
authors.forename: Mihaela
authors.initials: M
authors.lastname: Crisan
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
authors.forename: Lorenz
authors.initials: L
authors.lastname: Lehr
authors.forename: Mamen
authors.initials: M
authors.lastname: Carmona
authors.forename: Ariane
authors.initials: A
authors.lastname: Paoloni-Giacobino
authors.forename: Solomon
authors.initials: S
authors.lastname: Yap
authors.forename: Bin
authors.initials: B
authors.lastname: Sun
authors.forename: Bertrand
authors.initials: B
authors.lastname: Léger
authors.forename: Alison
authors.initials: A
authors.lastname: Logar
authors.forename: Luc
authors.initials: L
authors.lastname: Pénicaud
authors.forename: Patrick
authors.initials: P
authors.lastname: Schrauwen
authors.forename: David
authors.initials: D
authors.lastname: Cameron-Smith
authors.forename: Aaron Paul
authors.initials: AP
authors.lastname: Russell
authors.forename: Bruno
authors.initials: B
authors.lastname: Péault
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Giacobino
mesh_terms: D052437:Adipocytes, Brown / Q000166:cytology* / Q000378:metabolism; D000328:Adult; D000368:Aged; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism; D002454:Cell Differentiation; D002478:Cells, Cultured; D005260:Female; D005333:Fetus; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D008297:Male; D051379:Mice; D008875:Middle Aged; D024101:Mitochondrial Proteins / Q000378:metabolism; D018482:Muscle, Skeletal / Q000166:cytology* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D010085:Oxidative Phosphorylation; D010101:Oxygen Consumption; D047495:PPAR gamma / Q000819:agonists / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D000071256:Uncoupling Protein 1
chemical_list: D018952:Antigens, CD34; D007473:Ion Channels; D024101:Mitochondrial Proteins; D047495:PPAR gamma; C000604371:UCP1 protein, human; C000604372:Ucp1 protein, mouse; D000071256:Uncoupling Protein 1