[FK228 promotes the IL-3 mediated proliferation and differentiation of human erythroid progenitor cells].
OBJECTIVE
To investigate the role of histone deacetylases (HDACs) inhibitor FK228 in the IL-3 mediated proliferation and differentiation of human erythroid progenitor cells.


METHODS
CD34(+) cells were separated from the granulocyte colony-stimulating factor(G-CSF)-mobilized peripheral blood of cancer patients and cultured for 7 days with FK228 of different concentrations, stem cell factor(SCF) and interleukin 3 (IL-3) in serum-free medium. Flow cytometry was performed to analyze the expression of CD14, GPA, CD15 and CD36 on the cells and the colony assay of erythroid cells was conducted. Then the CD36(+)GPA(-) cells were enriched and cultured with with IL-3 and 0.5 microg/L FK228 supplemented serum-free semisolid cultures and the formation test of erythroid colony was conducted.


RESULTS
FK228 enhanced the generation of CD36(+) cells in a dose-dependent manner, in spite of that it displayed no influence on the generation of CD14(+) and CD15(+) cells. 0.5 g/L FK228 induced a remarkable increase in the cell number of CD36(+) cells cultured with IL-3; FK228 also significantly increased the number and size of CD36(+) cells colony cultured with IL-3 containing semisolid cultures. Moreover, FK228 augmented the formation of CD36(+) cells colony from CD36(+)GPA(-) cells cultured with IL-3.


CONCLUSIONS
FK228 can promote the IL-3 mediated proliferation and differentiation of human erythroid progenitor cells.
Yu
Zhao-Cai
ZC
Department of Oncology, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, China.
Liu
Wen-Chao
WC
Liu
Du-Hu
DH
Huang
Ying
Y
Shi
Xiao-Ming
XM
You
Xiang-Hui
XH
Chen
Yan
Y
D018955:CD36 Antigens / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D047630:Depsipeptides / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005434:Flow Cytometry; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology*; D006801:Humans; D007377:Interleukin-3 / Q000494:pharmacology*; D016256:Lewis X Antigen / Q000378:metabolism; D018950:Lipopolysaccharide Receptors / Q000378:metabolism
D018955:CD36 Antigens; D047630:Depsipeptides; D056572:Histone Deacetylase Inhibitors; D007377:Interleukin-3; D016256:Lewis X Antigen; D018950:Lipopolysaccharide Receptors; C087123:romidepsin