title: Juice of eclipta prostrata inhibits cell migration in vitro and exhibits anti-angiogenic activity in vivo.
abstract: BACKGROUND
  The invasion of cancer cells is critical for metastasis. The effects of Eclipta prostrata, a Thai medicinal plant, on invasion, migration and adhesion of cancer cells were investigated and the anti-angiogenic activity in vivo was evaluated.
  
  
  METHODS
  In vitro invasion and migration assays were performed in modified Boyden chambers. In vivo anti-angiogenic activity was determined using the chick chorioallantoic membrane (CAM) assay.
  
  
  RESULTS
  E. prostrata juice inhibited cancer invasion and migration, without affecting cell adhesion. Cell migration was inhibited in a variety of cancer cell types and in endothelial cells, with IC50 values of 31-70 microg/ml, much lower than the IC50 values for cytotoxicity of 203-1,217 microg/ml for cancer cells and >4,000 microg/ml for endothelial cells. Fifty percent inhibition of angiogenesis by E. prostrata juice was observed at 200 microg/egg.
  
  
  CONCLUSIONS
  E. prostrata juice inhibited cancer and endothelial cell migration in vitro and also showed in vivo anti-angiogenic activity.
authors.affiliation: Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailand.
authors.forename: Kriengsak
authors.initials: K
authors.lastname: Lirdprapamongkol
authors.forename: Jan-Peter
authors.initials: JP
authors.lastname: Kramb
authors.forename: Daranee
authors.initials: D
authors.lastname: Chokchaichamnankit
authors.forename: Chantragan
authors.initials: C
authors.lastname: Srisomsap
authors.forename: Rudee
authors.initials: R
authors.lastname: Surarit
authors.forename: Monnipha
authors.initials: M
authors.lastname: Sila-Asna
authors.forename: Ahnond
authors.initials: A
authors.lastname: Bunyaratvej
authors.forename: Gerd
authors.initials: G
authors.lastname: Dannhardt
authors.forename: Jisnuson
authors.initials: J
authors.lastname: Svasti
mesh_terms: D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002642:Chick Embryo; D036601:Eclipta / Q000737:chemistry*; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D009369:Neoplasms / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
chemical_list: D020533:Angiogenesis Inhibitors; D010936:Plant Extracts