Exposure to coal combustion residues during metamorphosis elevates corticosterone content and adversely affects oral morphology, growth, and development in Rana sphenocephala.
Coal combustion residues (CCRs) are documented to negatively impact oral morphology, growth, and development in larval amphibians. It is currently unclear what physiological mechanisms may mediate these effects. Corticosterone, a glucocorticoid hormone, is a likely mediator because when administered exogenously it, like CCRs, also negatively influences oral morphology, growth, and development in larval amphibians. In an attempt to identify if corticosterone mediates these effects, we raised larval Southern Leopard Frogs, Rana sphenocephala, on either sand or CCR substrate and documented effects of sediment type on whole body corticosterone, oral morphology, and time to and mass at key metamorphic stages. Coal combustion residue treated tadpoles contained significantly more corticosterone than controls throughout metamorphosis. However, significantly more oral abnormalities occurred early in metamorphosis when differences in corticosterone levels between treatments were minimal. Overall, CCR-treated tadpoles took significantly more time to transition between key stages and gained less mass between stages than controls, but these differences between treatments decreased during later stages when corticosterone differences between treatments were greatest. Our results suggest endogenous increase in corticosterone content and its influence on oral morphology, growth and development is more complex than previously thought.
Peterson
John D
JD
Department of Biological Sciences, Auburn University, Auburn, AL 36849, USA. peterj1@auburn.edu
Peterson
Vikki A
VA
Mendonça
Mary T
MT
D000818:Animals; D003031:Coal; D003345:Corticosterone / Q000378:metabolism*; D019015:Geologic Sediments; D017745:Incineration; D007814:Larva / Q000187:drug effects / Q000378:metabolism; D008675:Metamorphosis, Biological / Q000187:drug effects*; D009056:Mouth Abnormalities / Q000139:chemically induced* / Q000378:metabolism; D011894:Rana pipiens / Q000002:abnormalities* / Q000254:growth & development / Q000378:metabolism; D013997:Time Factors; D015854:Up-Regulation; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D003031:Coal; D014874:Water Pollutants, Chemical; D003345:Corticosterone