title: Genetic variants of microsomal epoxide hydrolase and glutamate-cysteine ligase in COPD.
abstract: The genetic factors that contribute to the development of chronic obstructive pulmonary disease (COPD) are poorly understood. Many candidate genes have been proposed, including enzymes that protect the lung against oxidative stress, such as microsomal epoxide hydrolase (EPHX1) and glutamate-cysteine ligase (GCL). To date, most reported findings have been for EPHX1, particularly in relation to functional variants associated with fast and slow metabolism of epoxide intermediates. The present study aimed to identify any association of variation in these genes with COPD susceptibility or severity. In total, 1,017 white COPD patients and 912 nondiseased age and sex matched smoking controls were genotyped for six single nucleotide polymorphisms (SNPs) in EPHX1 (including the fast and slow variants and associated haplotypes), and eight SNPs in the two genes encoding GCL. GCL is a rate-limiting enzyme in the synthesis of glutathione, a major contributor to anti-oxidant protection in the lung. No association of variation was found in EPHX1 or GCL with susceptibility to COPD or disease severity. This is the largest reported study to date and is well powered to detect associations that have been previously suggested. The current data indicate that these genetic variants are unlikely to be related to susceptibility or disease severity in white chronic obstructive pulmonary disease patients.
authors.affiliation: Division of Clinical Chemistry, University Hospital, University of Nottingham, Queens Medical Centre, Nottingham, UK.
authors.forename: S
authors.initials: S
authors.lastname: Chappell
authors.forename: L
authors.initials: L
authors.lastname: Daly
authors.forename: K
authors.initials: K
authors.lastname: Morgan
authors.forename: T
authors.initials: T
authors.lastname: Guetta-Baranes
authors.forename: J
authors.initials: J
authors.lastname: Roca
authors.forename: R
authors.initials: R
authors.lastname: Rabinovich
authors.forename: J
authors.initials: J
authors.lastname: Lotya
authors.forename: A B
authors.initials: AB
authors.lastname: Millar
authors.forename: S C
authors.initials: SC
authors.lastname: Donnelly
authors.forename: V
authors.initials: V
authors.lastname: Keatings
authors.forename: W
authors.initials: W
authors.lastname: MacNee
authors.forename: J
authors.initials: J
authors.lastname: Stolk
authors.forename: P S
authors.initials: PS
authors.lastname: Hiemstra
authors.forename: M
authors.initials: M
authors.lastname: Miniati
authors.forename: S
authors.initials: S
authors.lastname: Monti
authors.forename: C M
authors.initials: CM
authors.lastname: O'Connor
authors.forename: N
authors.initials: N
authors.lastname: Kalsheker
mesh_terms: D016022:Case-Control Studies; D004851:Epoxide Hydrolases / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D014644:Genetic Variation; D005838:Genotype; D005721:Glutamate-Cysteine Ligase / Q000235:genetics*; D005978:Glutathione / Q000378:metabolism; D006239:Haplotypes; D006801:Humans; D008297:Male; D020641:Polymorphism, Single Nucleotide; D029424:Pulmonary Disease, Chronic Obstructive / Q000235:genetics*; D012307:Risk Factors; D012907:Smoking
chemical_list: D004851:Epoxide Hydrolases; D005721:Glutamate-Cysteine Ligase; D005978:Glutathione