Time-suppression test using a colorimetric probe (alamarBlue) that measures bacterial metabolic activity.
We developed a time-suppression test using alamarBlue, which will allow estimation of the cidal or static nature of antimicrobials very easily and quickly. As an example, the effects of vancomycin, linezolid, and daptomycin on a representative strain of Staphylococcus aureus were estimated.
Komatsu
Mitsutaka
M
Department of Pediatrics, Juntendo University, Tokyo, Japan.
Tajima
Yutaka
Y
Ito
Teruyo
T
Yamashiro
Yuichiro
Y
Hiramatsu
Keiichi
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003124:Colorimetry; D010078:Oxazines; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism*; D014966:Xanthenes
D000900:Anti-Bacterial Agents; D010078:Oxazines; D014966:Xanthenes; C005843:resazurin