Vaccination of shrimp (Penaeus chinensis) against white spot syndrome virus (WSSV).
Two structural protein genes, VP19 and VP466, of white spot syndrome virus (WSSV) were cloned and expressed in Sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against WSSV for shrimps. The cumulative mortalities of the shrimps vaccinated by the injection of rVP19 and rVP466 at 15 days after the challenge with WSSV were 50.2% and 51.8%, respectively. For the vaccination by oral feeding of rVP19 and rVP466, the cumulative mortalities were 49.2% and 89.2%, respectively. These results show that protection against WSSV can be generated in the shrimp, using the viral structural protein as a protein vaccine.
Ha
Yu-Mi
YM
Department of Biotechnology and Bioengineering,Pukyong National University, Busan 608-737, Korea.
Soo-Jung
Gong
G
Thi-Hoai
Nguyen
N
Ra
Chae Hun
CH
Kim
Ki-Hong
KH
Nam
Yoon-Kwon
YK
Kim
Sung-Koo
SK
D000818:Animals; D002460:Cell Line; D015870:Gene Expression; D033561:Penaeidae / Q000276:immunology* / Q000821:virology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D018411:Spodoptera; D014611:Vaccination; D015678:Viral Structural Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D046848:White spot syndrome virus 1 / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
D011994:Recombinant Proteins; D015678:Viral Structural Proteins; D014765:Viral Vaccines