title: Urinary trypsin inhibitor improves viability of the liver in brain-dead rats.
abstract: OBJECTIVE
  The present study examined the effect of urinary trypsin inhibitor (UTI) on liver injury in hypotensive brain-dead rats.
  
  
  METHODS
  Brain death was induced by inflating a balloon catheter placed in the epidural space. UTI (100,000 units/kg/hour) was intravenously administered from 30 min until 6 hours after the induction of brain death. Systemic hemodynamics and hepatic tissue flow (HTF) were measured, and blood samples and hepatic tissue specimens for morphological examinations were obtained during the experiments.
  
  
  RESULTS
  The induction of brain death caused a 30% decrease in both mean arterial pressure and HTF, and an increase in the serum transaminase level in comparison with sham-operated rats. Brain death also increased the serum concentration of cytokine-induced neutrophil chemoattractant (CINC) (4.4-fold), as well as the number of CINC-positive cells (4.4-fold) and sequestered neutrophils in the sinusoids (3.1-fold). Post-treatment of brain-dead rats with UTI restored the HTF and reduced serum transaminase level. UTI decreased plasma CINC level and the number of neutrophils and CINC-positive cells in the sinusoids.
  
  
  CONCLUSIONS
  The results suggest that treatment with UTI after the establishment of brain death improved the viability of the liver in hypotensive brain-dead rats by inhibiting CINC production.
authors.affiliation: Department of Surgery, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 228-8555, Japan. itako@kitasato-u.ac.jp
authors.forename: Koichi
authors.initials: K
authors.lastname: Itabashi
authors.forename: Kazunori
authors.initials: K
authors.lastname: Furuta
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takahashi
authors.forename: Yoshiya
authors.initials: Y
authors.lastname: Ito
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Katagiri
authors.forename: Koshi
authors.initials: K
authors.lastname: Sato
authors.forename: Ahira
authors.initials: A
authors.lastname: Kakita
authors.forename: Masahiko
authors.initials: M
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D001926:Brain Death; D006023:Glycoproteins / Q000627:therapeutic use*; D008099:Liver / Q000187:drug effects* / Q000502:physiology*; D008297:Male; D051381:Rats; D011917:Rats, Inbred Lew; D014022:Tissue Survival / Q000187:drug effects*; D014361:Trypsin Inhibitors / Q000627:therapeutic use*
chemical_list: D006023:Glycoproteins; D014361:Trypsin Inhibitors; C028665:urinastatin