Protein synthesis in vitro in cultures of the subepidermal adipose tissue and the ovary of the shrimp Penaeus semisulcatus.
In vitro culture systems were developed for subepidermal adipose tissue (SAT) and ovary of the shrimp Penaeus semisulcatus. Both tissues were cultured in sea water-based media buffered with HEPES to pH 7.4 in an oxygen enriched atmosphere. Various incubation conditions were tested in order to define those supporting optimal rates of protein synthesis. Best results for de novo protein synthesis were obtained when amino acids and other supplements were added according to Landureau's medium composition for the SAT and Eagle's MEM for the ovary. Streptomycin was found to inhibit protein synthesis in SAT cultures. These in vitro systems are appropriate for future studies of serum lipoprotein synthesis and its hormonal control.
Fainzilber
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Israel Oceanographic & Limnological Research, P.O. Box 8030, Haifa 31080, Israel; Department of Entomology, Faculty of Agriculture, Hebrew University of Jerusalem, Rehovot, Israel.
Browdy
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Tom
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Lubzens
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Applebaum
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