Frequency of Giardia intestinalis assemblages isolated from dogs and humans in a community from Culiacan, Sinaloa, Mexico using beta-giardin restriction gene.
The assemblage of 37 Giardia intestinalis samples was determined, 19 obtained from puppy feces, 12 from stools of different human subjects under 3 years of age and 6 from axenic culture. The assemblages were classified according to the restriction pattern of beta-giardin gene with Hae III enzyme. Results showed that dog assemblages were grouped AI (52.6%) and AII (47.4%), while 41.7% human samples belonged to genotype AI and 58.3% to genotype AII. All axenic cultures belonged to assemblage AI; types AI and AII were both found in dog and human feces by Hae III restriction enzyme assay, suggesting a similarity between human and dog parasites. These results suggest that domestic animals infected with Giardia could produce cysts potentially infective for humans.
Eligio-García
Leticia
L
Laboratorio de Investigación en Parasitología, Hospital Infantil de México FG, Dr. Márquez No. 162, Col. Doctores, 06720 México, D.F., Mexico.
Cortes-Campos
Adrián
A
Cota-Guajardo
Silvia
S
Gaxiola
Soyla
S
Jiménez-Cardoso
Enedina
E
D000818:Animals; D002675:Child, Preschool; D003598:Cytoskeletal Proteins / Q000235:genetics*; D015252:Deoxyribonucleases, Type II Site-Specific / Q000378:metabolism; D004283:Dog Diseases / Q000453:epidemiology / Q000469:parasitology*; D004285:Dogs; D005243:Feces / Q000469:parasitology; D005260:Female; D016829:Giardia lamblia / Q000145:classification* / Q000235:genetics; D005873:Giardiasis / Q000469:parasitology / Q000662:veterinary*; D006801:Humans; D007223:Infant; D008297:Male; D008800:Mexico / Q000453:epidemiology; D015800:Protozoan Proteins / Q000235:genetics*
D003598:Cytoskeletal Proteins; D015800:Protozoan Proteins; C038207:giardin protein, Giardia lamblia; D015252:Deoxyribonucleases, Type II Site-Specific; C024403:GGCC-specific type II deoxyribonucleases