title: [Impact of benzo [a] pyrene the expression of mitochondrion-encoded genes in the earthworm Eisenia fetida].
abstract: The earthworm Eisenia fetida's benzo [a] pyrene (BaP) exposure experiments were carried out in artificial soil according to ISO 11268-1:1993. And then the upregulated and downregulated subtractive cDNA libraries were constructed by Clontech PCR-Select cDNA Subtration Kit. From the BaP exposure upregulated subtractive cDNA library, several cDNA segments matched mitochondrion-encoded genes were found, including cytochrome c oxidase subunit I (CO I), subunit II (CO II), subunit Ill (CO III), NADH dehydrogenase subunit 1 (NDH1), and ATP synthase subunit 6. The result indicated BaP and the subsequent oxidative stress disturbed the expression of mitochondrion-encoded genes, and this was potential biomarker for oxidative stress following xenobiotic exposure.
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. zhengsenlin@yahoo.com.cn
authors.forename: Sen-lin
authors.initials: SL
authors.lastname: Zheng
authors.forename: Yu-fang
authors.initials: YF
authors.lastname: Song
authors.forename: Xiao-yan
authors.initials: XY
authors.lastname: Qiu
authors.forename: Tie-heng
authors.initials: TH
authors.lastname: Sun
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.forename: M L
authors.initials: ML
authors.lastname: Ackland
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D050259:Genes, Mitochondrial / Q000187:drug effects* / Q000235:genetics; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D009835:Oligochaeta / Q000187:drug effects* / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012989:Soil Pollutants / Q000633:toxicity*
chemical_list: D004272:DNA, Mitochondrial; D012989:Soil Pollutants; D001564:Benzo(a)pyrene; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; C412204:ATP synthase subunit 6; D025261:Mitochondrial Proton-Translocating ATPases