title: Caffeine monitoring in infants: comparison of automated (VITROS 5, 1 FS) chemistry system versus HPLC analysis.
abstract: This is the first study comparing the caffeine testing by HPLC to the MicroTip Technology patented by Ortho-Clinical Diagnostics. For the determination of the precision for intra-run and day to day variances, control materials with concentration ranges between 2.3 microg/mL and 23.3 microg/mL were used. Test evaluation was done using plasma samples. The coefficient of variation for intra-run precision was calculated to range from 4.3% to 2.1%. The coefficients of variation for the day-to-day precision were between 4.9% and 2.3%. A coefficient of correlation of 0.99% was calculated for the comparison of the two methods. In the statistical analysis of the comparison of the methods. Differences between + 4.5% and - 0.92% could be found. The HPLC system must be ready for use at any time necessitating maintenance and increased costs. This, in addition to the low sample throughput for caffeine analysis and the findings of this study favour the use of an automated clinical chemistry system.
authors.affiliation: Medical University Vienna, Department of Pediatrics, Division of Neonatology and Intensive Care Medicine, Vienna, Austria. reiter@lbe.co.at
authors.forename: Rosmarie B K
authors.initials: RB
authors.lastname: Reiter-Reisacher
authors.forename: Martin
authors.initials: M
authors.lastname: Wald
authors.forename: Manfred
authors.initials: M
authors.lastname: Weninger
authors.forename: Kurt R
authors.initials: KR
authors.lastname: Herkner
mesh_terms: D001774:Blood Chemical Analysis / Q000295:instrumentation* / Q000379:methods; D002110:Caffeine / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016903:Drug Monitoring / Q000379:methods*; D006801:Humans; D007231:Infant, Newborn; D015203:Reproducibility of Results
chemical_list: D002110:Caffeine