Survey of O-GlcNAc level variations in Xenopus laevis from oogenesis to early development.
Little is known about the impact of O-linked-N-acetylglucosaminylation (O-GlcNAc) in gametes production and developmental processes. Here we investigated changes in O-GlcNAc, UDP-GlcNAc and O-GlcNAc transferase (OGT) levels in Xenopus laevis from oogenesis to embryo hatching. We showed that in comparison to stage VI, stages I-V oocytes expressed higher levels of O-GlcNAc correlating changes in OGT expression, but not in UDP-GlcNAc pools. Upon progesterone stimulation, an O-GlcNAc level burst occurred during meiotic resumption long before MPF and Mos-Erk2 pathways activations. Finally, we observed high levels of O-GlcNAc, UDP-GlcNAc and OGT during segmentation that decreased concomitantly at the onset of gastrulation. Nevertheless, no correlation between the glycosylation, the nucleotide-sugar and the glycosyltransferase was observed after neurulation. Our results show that O-GlcNAc is regulated throughout oogenesis and development within a complex pattern and suggest that dysfunctions in the dynamics of this glycosylation could lead to developmental abnormalities.
Dehennaut
Vanessa
V
UMR-CNRS 8576, Unité de Glycobiologie Structurale et Fonctionnelle, IFR 147, USTL, 59655, Villeneuve d'Ascq, France.
Lefebvre
Tony
T
Leroy
Yves
Y
Vilain
Jean-Pierre
JP
Michalski
Jean-Claude
JC
Bodart
Jean-François
JF
D000117:Acetylglucosamine / Q000378:metabolism*; D000818:Animals; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D047108:Embryonic Development / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D016200:Maturation-Promoting Factor / Q000378:metabolism; D008540:Meiosis / Q000187:drug effects; D019950:Mitogen-Activated Protein Kinase 1 / Q000378:metabolism; D017351:N-Acetylglucosaminyltransferases / Q000378:metabolism; D009865:Oocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D009866:Oogenesis / Q000187:drug effects; D011374:Progesterone / Q000494:pharmacology; D014537:Uridine Diphosphate N-Acetylglucosamine / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology* / Q000378:metabolism*
D011374:Progesterone; D014537:Uridine Diphosphate N-Acetylglucosamine; D017351:N-Acetylglucosaminyltransferases; C449880:O-GlcNAc transferase; D016200:Maturation-Promoting Factor; D019950:Mitogen-Activated Protein Kinase 1; D000117:Acetylglucosamine