Bifidobacterium infantis suppresses proinflammatory interleukin-17 production in murine splenocytes and dextran sodium sulfate-induced intestinal inflammation.
Interleukin (IL)-17 acts as a potent inflammatory cytokine, and IL-17-producing cells (Th17 cells) have received much attention. However, the involvement of commensal and/or probiotic bacteria in IL-17 production has not been evaluated. In this study, we examined the suppressive effects of five bacteria species on IL-17 production in vitro and ex vivo. Among the five species studied, Bifidobacterium infantis inhibited IL-17 production but enhanced IL-27 production most potently in TGF-beta plus IL-6-stimulated murine splenocytes. B. infantis also inhibited IL-17 and eotaxin production from a dextran sodium sulfate-treated colon organ culture. The induction of IL-10 by B. infantis was observed both in the splenocytes and in the colon culture and was assumed, to a certain extent, to be important for suppressing IL-17 production. These findings suggest a novel immunomodulatory function of commensal bifidobacteria and further imply that these bacteria may be useful in the treatment of Th17-mediated diseases.
Tanabe
Soichi
S
Graduate School of Biosphere Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Hiroshima, Japan. stanabe@hiroshima-u.ac.jp
Kinuta
Yuki
Y
Saito
Yasuo
Y
D000818:Animals; D001644:Bifidobacterium / Q000276:immunology*; D002478:Cells, Cultured; D054413:Chemokine CCL11 / Q000378:metabolism; D018925:Chemokines / Q000378:metabolism; D016264:Dextran Sulfate / Q000276:immunology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D016753:Interleukin-10 / Q000276:immunology; D020381:Interleukin-17 / Q000276:immunology*; D007378:Interleukins / Q000276:immunology; D007422:Intestines / Q000033:anatomy & histology / Q000276:immunology* / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics; D013154:Spleen / Q000166:cytology* / Q000276:immunology; D046509:Tissue Culture Techniques
D054413:Chemokine CCL11; D018925:Chemokines; C486767:Il27 protein, mouse; D020381:Interleukin-17; D007378:Interleukins; D016753:Interleukin-10; D016264:Dextran Sulfate