[The influence of different glucose concentrations on cellular activity of human osteosarcoma cell line MG63].
OBJECTIVE
In this research, we took an insight into the influence of different glucose concentrations on human osteosarcoma cell line MG63, hoping to give an explanation to the mechanism of the osteoporosis (OP) caused by diabetes mellitus (DM).


METHODS
Four culture media of different glucose concentrations, 5.5 mmol/L, 10 mmol/L, 20 mmol/L, 40 mmol/L, were set up for this study. Human osteosarcoma cell line MG63 was cultured in these media successively for 21 d, and then the proliferation rate of cells, secretion of ALP and BGP, percentage of apoptosis cells and the formation of calcified nods were observed and measured.


RESULTS
The elevated glucose concentration prompted the proliferation of MG63 cells, but the activities of MG63 cells were not correspondent with the cell number. On the contrary, the secretion of ALP, BGP and the mineralization of the MG63 cells were decreased. The apoptosis of the cells was promoted.


CONCLUSIONS
From our study, we confirmed the toxicity of hyperglycemia to MG63 cells. It suggested that the tight control of the serum glucose can be a good method to prevent the occurrence of osteoporosis.
Cheng
Meng
M
Department of Endocrinology, Osteoporosis Education Center, West China Hospital, Sichuan University, Chengdu 610041, China.
Chen
De-Cai
DC
Lu
Chun-Yan
CY
D000469:Alkaline Phosphatase / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects; D001859:Bone Neoplasms / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005947:Glucose / Q000494:pharmacology*; D006801:Humans; D015675:Osteocalcin / Q000378:metabolism*; D012516:Osteosarcoma / Q000378:metabolism / Q000473:pathology
D015675:Osteocalcin; D000469:Alkaline Phosphatase; D005947:Glucose