1,2,3,4,6-penta-O-galloyl-beta-D-glucose from Galla Rhois protects primary rat hepatocytes from necrosis and apoptosis.
In this study, we investigated the hepatoprotective effects of four compounds from Galla Rhois [gallic acid methyl ester, gallic acid, an equilibrium mixture of 3-galloyl-gallic acid and 4-galloyl-gallic acid isomers, and 1,2,3,4,6-penta- O-galloyl- beta- D-glucose (PGG)] in primary rat hepatocytes undergoing necrosis or apoptosis. Treatment with gallic acid methyl ester (12.5 and 50 microM) or PGG (3.125, 12.5 and 50 microM) reduced hepatocyte necrosis induced by tert-butyl hydroperoxide. PGG treatment (4 and 20 microM) also altered hepatocyte apoptosis induced by glycochenodeoxycholic acid. Based on these results, we propose that PGG warrants further evaluation as a hepatoprotective agent, because it protected primary rat hepatocytes from both necrosis and apoptosis.
Park
Eun-Jeon
EJ
Institute of Pharmaceutical Research and Development, Department of Pharmacy, Wonkwang University, Iksan, Republic of Korea.
Zhao
Yu-Zhe
YZ
An
Ren-Bo
RB
Kim
Youn-Chul
YC
Sohn
Dong Hwan
DH
D000818:Animals; D001042:Aphids; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D022781:Hepatocytes / Q000187:drug effects*; D047348:Hydrolyzable Tannins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D009336:Necrosis / Q000517:prevention & control; D010941:Plant Tumors; D051381:Rats; D029045:Rhus / Q000737:chemistry
D047348:Hydrolyzable Tannins; C435084:pentagalloylglucose; C052082:methyl gallate; D005707:Gallic Acid