title: Chemiluminescence assay of mucosal reactive oxygen species in gastric cancer, ulcer and antral mucosa.
abstract: OBJECTIVE
  Reactive oxygen species (ROS) have been implicated in inflammatory and cancerous illness, including that of the gastrointestinal tract. The oxidative damage incurred during human gastric ulcer or cancer mucosa may be related to acumination of ROS. In this study, we aimed to demonstrate oxidative stress of gastric ulcer and cancer mucosa compared to gastric antral mucosa.
  
  
  METHODS
  
  
  
  METHODS
  Thirty-four patients with gastric ulcer and gastric cancer were enrolled in this study. Gastric mucosa specimens, taken from upper GI endoscopic biopsy, from the lesion (ulcer or cancer) and antrum were sent for the activity of O2- or H2O2 determined by chemiluminescence assay. Protein concentrations in the tissue homogenates were determined by Bio-Red protein assay. The production of O2- or H2O2 per unit of protein was calculated by dividing the tissue CL level by the protein content of a tissue.
  
  
  RESULTS
  The oxidative stress metabolites O2- and H2O2 of mucosa were evaluated by chemiluminescence assay in gastric lesions (27 ulcers and 7 cancers) and gastric antrum. Gastric lesion showed significantly increased O2- than antral mucosa (18.77 +/- 45.18 (counts/sec x microg), 95% CI 3.01, 34.53 vs. 3.58 +/- 6.89 (counts/sec x microg), 95% CI 1.18, 5.98, p < 0.05). There was also significantly greater expression of H2O2 in gastric lesion than gastric antral mucosa (76.06 +/- 148.36 (counts/sec x microg), 95% CI 24.30, 127.83 vs. 912.41 +/- 20.22 (counts/sec x microg), 95% CI 5.35, 19.46, p = 0.008). Differences of mucosal O2- and H2O2 between gastric ulcer and cancer were not significant. There was significant correlation of O2- and H2O2 generation in gastric lesion mucosa.
  
  
  CONCLUSIONS
  Oxidative stress is now thought to make a significant contribution to inflammatory disease and malignancy. The reason that overproduction of free radicals is a feature of such a broad spectrum of diseases derived from the fact that oxidative metabolism is a necessary part of every cell's metabolism. In this study, we demonstrated increased ROS production in gastric ulceration and cancer compared with gastric antral mucosa.
authors.affiliation: Department of Emergency Medicine, Taichung Veterans General Hospital, Taichung, Taiwan. pychunppp@gmail.com
authors.forename: Yen-Chun
authors.initials: YC
authors.lastname: Peng
authors.forename: Chia-Lang
authors.initials: CL
authors.lastname: Hsu
authors.forename: Chun-Fang
authors.initials: CF
authors.lastname: Tung
authors.forename: Wai-Keung
authors.initials: WK
authors.lastname: Chou
authors.forename: Lan-Ru
authors.initials: LR
authors.lastname: Huang
authors.forename: Dong-Zong
authors.initials: DZ
authors.lastname: Hung
authors.forename: Wei-Hsiung
authors.initials: WH
authors.lastname: Hu
authors.forename: Dar-Yu
authors.initials: DY
authors.lastname: Yang
mesh_terms: D005753:Gastric Mucosa / Q000737:chemistry* / Q000378:metabolism*; D006801:Humans; D008163:Luminescent Measurements; D011706:Pyloric Antrum / Q000737:chemistry* / Q000378:metabolism*; D017382:Reactive Oxygen Species / Q000032:analysis*; D013274:Stomach Neoplasms / Q000737:chemistry* / Q000378:metabolism*; D013276:Stomach Ulcer / Q000378:metabolism*
chemical_list: D017382:Reactive Oxygen Species