[Growth and differentiation of cell hybrids obtained by fusing mouse PCC4azal teratocarcinoma cells and mouse spleen cells under different in vitro culture conditions].
Cell hybrids obtained by fusing mouse PCC4azal teratocarcinoma cells and spleen cells induced to proliferation and treated with the demethylating agent 5-azacytidine prior to fusion are described. The obtained hybrids demonstrated no expression of T lymphocyte marker genes CD11 and CD45, which indicates possible somatic nucleus reprogramming by factors present in teratocarcinoma cells. Irrespective of culture conditions, cell hybrids demonstrated a relatively stable chromosome number: they lost on average no more than four chromosomes after 30 passages. Culturing in medium containing hypoxanthine, aminopterin, and thymidine (selective conditions) decreased the differentiation capacity of cell hybrids compared to nonselective conditions, which is likely due to the inhibition of their metabolism. For the first time, teratocarcinoma cell hybrid differentiation into cardiomyocytes under the influence of DMSO has been demonstrated in vitro.
Filiasova
E I
EI
Zatsepina
O V
OV
Khodarovich
Iu M
IuM
Larionov
O A
OA
D000818:Animals; D000943:Antigens, Differentiation / Q000096:biosynthesis; D001374:Azacitidine / Q000494:pharmacology; D018845:CD11 Antigens / Q000096:biosynthesis; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects*; D002459:Cell Fusion / Q000379:methods; D045744:Cell Line, Tumor; D033481:Chromosomes, Mammalian / Q000235:genetics; D003451:Cryoprotective Agents / Q000494:pharmacology*; D019175:DNA Methylation / Q000187:drug effects; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004791:Enzyme Inhibitors / Q000494:pharmacology; D006825:Hybridomas / Q000378:metabolism* / Q000473:pathology; D017493:Leukocyte Common Antigens / Q000096:biosynthesis; D051379:Mice; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D013154:Spleen / Q000378:metabolism* / Q000473:pathology; D013601:T-Lymphocytes / Q000378:metabolism / Q000473:pathology; D018243:Teratocarcinoma / Q000378:metabolism* / Q000473:pathology
D000943:Antigens, Differentiation; D018845:CD11 Antigens; D003451:Cryoprotective Agents; D004791:Enzyme Inhibitors; D017493:Leukocyte Common Antigens; C516770:Ptprc protein, mouse; D001374:Azacitidine; D004121:Dimethyl Sulfoxide