Molecular diversity and phylogeny of Hantaan virus in Guizhou, China: evidence for Guizhou as a radiation center of the present Hantaan virus.
To gain further insight into the molecular epidemiology of Hantaan virus (HTNV) in Guizhou, China, rodents were captured in this region in 2004 and 2005. In addition, serum samples were collected from four patients. Ten hantaviruses were isolated successfully in cell culture from four humans, two Apodemus agrarius, three Rattus norvegicus and one Rattus nitidus. The nucleotide sequences for their small (S), medium (M) and partial large (L) segments were determined. Phylogenetic analysis of the S and M segment sequences revealed that all of these isolates belong to the species HTNV, suggesting a spillover of HTNV from A. agrarius to Rattus rats. All available isolates from Guizhou were divided into four distinct groups either in the S segment tree or in the M segment tree. The clustering pattern of these isolates in the S segment tree was not in agreement with that in the M or L segment tree, showing that genetic reassortment between HTNV had occurred naturally. Analysis of the S segment sequences from available HTNV strains indicated that they formed three clades. The first clade, which comprised only viruses from Guizhou, was the outgroup of clades II and III. The viruses in the second clade were found in Guizhou and mainly in the far-east Asian region, including China. However, the viruses in the third clade were found in most areas of China, including Guizhou, in which haemorrhagic fever with renal syndrome (HFRS) is endemic. Our results reveal that the highest genetic diversity of HTNV is in a limited geographical region of Guizhou, and suggest that Guizhou might be a radiation centre of the present form of HTNV.
Zou
Yang
Y
Beijing Friendship Hospital, Affiliate of Capital Medical University, Beijing, PR China.|Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
Hu
Jing
J
Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou Province, PR China.
Wang
Zhao-Xiao
ZX
Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou Province, PR China.
Wang
Ding-Ming
DM
Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou Province, PR China.
Li
Ming-Hui
MH
Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
Ren
Guo-Dong
GD
Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
Duan
Zheng-Xiu
ZX
Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
Fu
Zhen F
ZF
Department of Pathology, University of Georgia, Athens, GA 30602, USA.
Plyusnin
Alexander
A
Department of Virology, Haartman Institute, University of Helsinki, Finland.
Zhang
Yong-Zhen
YZ
Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
D000595:Amino Acid Sequence; D000818:Animals; D002681:China / Q000453:epidemiology; D002522:Chlorocebus aethiops; D014644:Genetic Variation; D018063:Hantaan virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology* / Q000662:veterinary / Q000821:virology; D006801:Humans; D008168:Lung / Q000821:virology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012376:Rodent Diseases / Q000453:epidemiology* / Q000821:virology; D017422:Sequence Analysis, DNA; D014709:Vero Cells