Oxidative stresses induced by glycoxidized human or bovine serum albumin on human monocytes.
Oxidative stress and protein modifications are frequently observed in numerous disease states. Albumin, the major circulating protein in blood, can undergo increased glycoxidation in diabetes. Protein glycoxidation can lead to the formation of advanced glycoxidation end products, which induce various deleterious effects on cells. Herein, we report the effect of glucose or methylglyoxal-induced oxidative modifications on BSA or HSA protein structures and on THP1 monocyte physiology. The occurrence of oxidative modifications was found to be enhanced in glycoxidized BSA and HSA, after determination of their free thiol group content, relative electrophoretic migration, carbonyl content, and antioxidant activities. Cells treated with glycoxidized albumin exhibited an overgeneration of intracellular reactive oxygen species, impairments in proteasomal activities, enhancements in RAGE expression, and an accumulation of carbonylated proteins. These novel observations made in the presence of a range of modified BSA and HSA facilitate the comparison of the glycoxidation extent of albumin with the oxidative stress induced in cultured monocytes. Finally, this study reconfirms the influence of experimental conditions in which AGEs are generated and the concentration levels in experiments designed to mimic pathological conditions.
Rondeau
Philippe
P
Laboratoire de Biochimie et Génétique Moléculaire (LBGM), Université de La Réunion, Saint Denis de La Réunion, 15, avenue René Cassin, BP 7151 Cedex 09, 97715 Saint Denis de La Réunion, France.
Singh
Nihar Ranjan
NR
Caillens
Henri
H
Tallet
Frank
F
Bourdon
Emmanuel
E
D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D002417:Cattle; D005947:Glucose / Q000737:chemistry*; D017127:Glycation End Products, Advanced; D006801:Humans; D006868:Hydrolysis; D009000:Monocytes / Q000187:drug effects* / Q000378:metabolism; D010084:Oxidation-Reduction; D018384:Oxidative Stress; D012709:Serum Albumin / Q000737:chemistry / Q000494:pharmacology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D000975:Antioxidants; D017127:Glycation End Products, Advanced; D012709:Serum Albumin; D005947:Glucose