title: Isolation and structural elucidation of different geometrical isomers of lycopene.
abstract: Five geometrical isomers of lycopene were isolated from a photoisomerized mixture on a semi-preparative C30 column and characterized by UV-vis spectroscopy, mass spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. 1H NMR and 2D NMR measurements were used to unambiguously assign the double bond configuration of five isomers: (5Z,9'Z)-, (9Z)-, (5Z,9Z)-, (all-E)-, and (5Z)-lycopene. This will allow other laboratories to use the results for their C30 HPLC. In addition, it is possible to investigate structure-activity relationships for these lycopene isomers which will improve the understanding of their physiological role in biological tissues.
authors.affiliation: Institute of Nutrition, Friedrich Schiller University Jena, Dornburger Strasse 25-29, Jena, Germany.
authors.forename: Kati
authors.initials: K
authors.lastname: Fröhlich
authors.forename: Jürgen
authors.initials: J
authors.lastname: Conrad
authors.forename: Antje
authors.initials: A
authors.lastname: Schmid
authors.forename: Dietmar E
authors.initials: DE
authors.lastname: Breithaupt
authors.forename: Volker
authors.initials: V
authors.lastname: Böhm
mesh_terms: D002338:Carotenoids / Q000737:chemistry* / Q000302:isolation & purification*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D007536:Isomerism; D000077276:Lycopene; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D013058:Mass Spectrometry / Q000379:methods; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D002338:Carotenoids; D000077276:Lycopene