title: Anti-tumor effect of DNA-based vaccination and dSLIM immunomodulatory molecules in mice with Ph+ acute lymphoblastic leukaemia.
abstract: Since the prognosis of patients with Philadelphia chromosome positive acute lymphoblastic leukaemia (Ph+ ALL) still remains poor, new relapse prevention strategies are needed. We evaluated the pre-immunization of mice with DNA-based vaccines subsequently challenged by the syngeneic Ph+ ALL cell line BM185. Ballistic transfer of minimalistic immunogenically defined gene expression (MIDGE) vectors encoding a BCR-ABLp185 fusion specific peptide or GM-CSF were used for in vivo transfection. DNA-based double stem-loop immunomodulators (dSLIM) were used as immune adjuvant. We present survival and functional data that DNA-based vaccination with BCR-ABLp185 fusion specific sequences, GM-CSF and dSLIM leads to an anti-tumor effect in mice challenged with a lethal Ph+ ALL dose and this effect depends on leukaemia-specific sequences.
authors.affiliation: Department of Paediatrics, Universität zu Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany. koechling@paedia.ukl.mu-luebeck.de
authors.forename: Joachim
authors.initials: J
authors.lastname: Köchling
authors.forename: Javier
authors.initials: J
authors.lastname: Prada
authors.forename: Masoud
authors.initials: M
authors.lastname: Bahrami
authors.forename: Renata
authors.initials: R
authors.lastname: Stripecke
authors.forename: Karl
authors.initials: K
authors.lastname: Seeger
authors.forename: Günter
authors.initials: G
authors.lastname: Henze
authors.forename: Burghardt
authors.initials: B
authors.lastname: Wittig
authors.forename: Manuel
authors.initials: M
authors.lastname: Schmidt
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D005260:Female; D005822:Genetic Vectors; D007155:Immunologic Factors / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D011119:Polynucleotides / Q000008:administration & dosage*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D055502:Secondary Prevention; D016019:Survival Analysis; D014162:Transfection / Q000379:methods; D019444:Vaccines, DNA / Q000276:immunology*
chemical_list: D019496:Cancer Vaccines; D007155:Immunologic Factors; D011119:Polynucleotides; D019444:Vaccines, DNA