title: Characterization and quantification of grape variety by means of shikimic acid concentration and protein fingerprint in still white wines.
abstract: Protein profiles, obtained by high-performance capillary electrophoresis (HPCE) on white wines previously dialyzed, combined with shikimic acid concentration and multivariate analysis, were used for the determination of grape variety composition of a still white wine. Six varieties were studied through monovarietal wines elaborated in the laboratory: Chardonnay (24 samples), Chenin (24), Petit Manseng (7), Sauvignon (37), Semillon (24), and Ugni Blanc (9). Homemade mixtures were elaborated from authentic monovarietal wines according to a Plackett-Burman sampling plan. After protein peak area normalization, a matrix was elaborated containing protein results of wines (mixtures and monovarietal). Partial least-squares processing was applied to this matrix allowing the elaboration of a model that provided a varietal quantification precision of around 20% for most of the grape varieties studied. The model was applied to commercial samples from various geographical origins, providing encouraging results for control purposes.
authors.affiliation: Service Commun des Laboratoires, Laboratoire de Pessac, 3 avenue du Dr. A. Schweitzer, 33408 Pessac, France.
authors.forename: David
authors.initials: D
authors.lastname: Chabreyrie
authors.forename: Serge
authors.initials: S
authors.lastname: Chauvet
authors.forename: François
authors.initials: F
authors.lastname: Guyon
authors.forename: Marie-Hélène
authors.initials: MH
authors.lastname: Salagoïty
authors.forename: Jean-François
authors.initials: JF
authors.lastname: Antinelli
authors.forename: Bernard
authors.initials: B
authors.lastname: Medina
mesh_terms: D019075:Electrophoresis, Capillary; D005638:Fruit / Q000737:chemistry* / Q000145:classification*; D010449:Peptide Mapping / Q000379:methods; D010940:Plant Proteins / Q000032:analysis; D012765:Shikimic Acid / Q000032:analysis*; D027843:Vitis / Q000145:classification*; D014920:Wine / Q000032:analysis* / Q000145:classification
chemical_list: D010940:Plant Proteins; D012765:Shikimic Acid