title: Prevalence of herpes simplex virus type 1 glycoprotein G (gG) and gI genotypes in patients with herpetic keratitis.
abstract: OBJECTIVE
  Recent phylogenetic analyses on the herpes simplex virus type 1 (HSV-1) genes US4, encoding glycoprotein G (gG) and US7, encoding gI, of clinical HSV-1 isolates have led to the classification of HSV-1 into three genotypes, arbitrarily designated as A, B and C. The prevalence of the HSV-1 gG and gI genotypes and their potential disease association was determined in a large cohort of patients with herpetic keratitis (HK).
  
  
  METHODS
  Primary corneal HSV-1 isolates of 178 HK patients were genotyped by a PCR-based restriction fragment length polymorphism method targeting the viral genes US4 and US7.
  
  
  RESULTS
  Genotype B was more frequently expressed by the corneal HSV-1 isolates compared with genotypes A and C. Fifty-five of 178 corneal isolates (31%) had different genotypes in both loci. No clinically relevant associations were observed between the HSV-1 genotypes and disease outcome in the HK patients studied.
  
  
  CONCLUSIONS
  The data presented demonstrate a high frequency of recombinant corneal HSV-1 isolates and suggest that clinical outcome of HSV-1-induced keratitis is independent of a gG or gI genotype.
authors.affiliation: Department of Virology, Erasmus Medical Center, Rotterdam, The Netherlands.
authors.forename: R
authors.initials: R
authors.lastname: Duan
authors.forename: J M
authors.initials: JM
authors.lastname: van Dun
authors.forename: L
authors.initials: L
authors.lastname: Remeijer
authors.forename: M
authors.initials: M
authors.lastname: Siemerink
authors.forename: P G H
authors.initials: PG
authors.lastname: Mulder
authors.forename: P
authors.initials: P
authors.lastname: Norberg
authors.forename: A D M E
authors.initials: AD
authors.lastname: Osterhaus
authors.forename: G M G M
authors.initials: GM
authors.lastname: Verjans
mesh_terms: D000368:Aged; D015331:Cohort Studies; D004279:DNA, Viral / Q000235:genetics / Q000302:isolation & purification; D005260:Female; D005838:Genotype; D018259:Herpesvirus 1, Human / Q000145:classification / Q000235:genetics*; D006801:Humans; D016849:Keratitis, Herpetic / Q000821:virology*; D008297:Male; D008875:Middle Aged; D009426:Netherlands; D012150:Polymorphism, Restriction Fragment Length; D014759:Viral Envelope Proteins / Q000032:analysis / Q000235:genetics*
chemical_list: D004279:DNA, Viral; D014759:Viral Envelope Proteins; C064959:glycoprotein I, herpes simplex virus type 1; C050591:glycoprotein gG-1, herpes simplex virus type 1