title: The DLC-1 -29A/T polymorphism is not associated with nasopharyngeal carcinoma risk in Chinese population.
abstract: Deleted in liver cancer-1 (DLC-1), encoding a Rho GTPase-activating protein (GAP), is considered as a promising candidate tumor suppressor gene in nasopharyngeal carcinoma (NPC). The single-nucleotide polymorphism (SNP) -29A/T upstream of ATG start codon was found when gene mutation profile of DLC-1 in NPC was analyzed. To evaluate the correlation between SNP -29A/T in the promoter region of DLC-1 gene and risk of NPC, a total of 521 samples from a Chinese population, including 320 healthy individuals and 201 NPC patients, were collected for SNP analysis by PCR-single-strand conformation polymorphism and sequencing. The differences in allele and genotype frequencies between NPC patients and controls were tested using logistic regression statistical method. No significant differences were found in allele or genotype frequencies between NPC patients and controls or among different NPC clinical stages. Hence, our data indicate that the SNP -29A/T of DLC-1 gene is not associated with NPC susceptibility.
authors.affiliation: Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Changsha, People's Republic of China.
authors.forename: Xiang-Ling
authors.initials: XL
authors.lastname: Feng
authors.forename: Wen
authors.initials: W
authors.lastname: Zhou
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.forename: Wei-Yi
authors.initials: WY
authors.lastname: Fang
authors.forename: Yi-Bo
authors.initials: YB
authors.lastname: Zhou
authors.forename: Kai-Tai
authors.initials: KT
authors.lastname: Yao
authors.forename: Cai-Ping
authors.initials: CP
authors.lastname: Ren
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D044466:Asian People / Q000235:genetics*; D002277:Carcinoma / Q000235:genetics; D016022:Case-Control Studies; D002681:China; D004252:DNA Mutational Analysis; D005260:Female; D020690:GTPase-Activating Proteins; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D018807:Polymorphism, Single-Stranded Conformational; D044382:Population Groups / Q000235:genetics*; D012307:Risk Factors; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: C112352:DLC1 protein, human; D020690:GTPase-Activating Proteins; D025521:Tumor Suppressor Proteins