Pectinase partitioning in polyethylene glycol 1000/Na2SO4 aqueous two-phase system: statistical modeling of the experimental results.
Statistical models concerning partitioning of pectinase in polyethylene glycol 1000/Na2SO4 aqueous two-phase system were established with response surface methodology. Concentrations of polyethylene glycol 1000 and Na2SO4 were selected as independent variables to evaluate their impact on parameters of partitioning in aqueous two-phase system--the partition coefficient of pectinase, purification factor and pectinase yield. An experimental space where over 2.5-fold purification was achieved, followed by over 90% yield of pectinase. The established models showed good prediction of partitioning parameters.
Antov
Mirjana
M
Faculty of Technology, University of Novi Sad, Blvd. Cara Lazara 1, Novi Sad, Serbia. mantov@uns.ns.ac.yu
Omorjan
Radovan
R
D000255:Adenosine Triphosphate / Q000378:metabolism; D015233:Models, Statistical; D011092:Polyethylene Glycols / Q000737:chemistry*; D011096:Polygalacturonase / Q000302:isolation & purification* / Q000378:metabolism; D013431:Sulfates / Q000737:chemistry*; D014867:Water
D013431:Sulfates; D014867:Water; C012036:sodium sulfate; D011092:Polyethylene Glycols; D000255:Adenosine Triphosphate; D011096:Polygalacturonase