A simple mechanical method to isolate the basal lamina of insect midgut epithelial cells.
In mealworms (Tenebrio molitor) the midgut epithelium is surrounded by a 1.6microm thick basal lamina of low electron density with a framework of high electron density imbedded in a part of it. The lamina can be isolated by ultrasonication followed by repeated filtrations and high-speed centrifugations, making large-scale preparation of the lamina for further analyses possible. The isolation of the basal lamina is confirmed by electron microscopy.
Koefoed
B M
BM
University Institute of Pathology, Odense University Hospital, Odense C, Denmark.