Control of neuronal persistent activity by voltage-dependent dendritic properties.
Neural integrators and working memory rely on persistent activity, a widespread neural phenomenon potentially involving persistent sodium conductances. Using a unique combination of voltage-clamp, dynamic-clamp, and frequency-domain techniques, we have investigated the role of voltage-dependent conductances on the dendritic electrotonic structure of neurons of the prepositus hypoglossi nucleus (PHN), which is known to be involved in oculomotor integration. The PHN contains two main neuronal populations: type B neurons with a double afterhyperpolarization and type D neurons, which not only are oscillatory but also have a greater electrotonic length than that of type B neurons. The persistent sodium conductance is present in all PHN neurons, although its effect on the dynamic electrotonic structure is shown to significantly differ in the two major cell types present in the nucleus. The electrotonic differences are such that the persistent sodium conductance can be almost perfectly manipulated in a type B neuron using an on-line dynamic clamp to add or subtract virtual sodium ion channels. The dynamic-clamp results are confirmed by data-fitted models, which suggest that the persistent sodium conductance has two different roles depending on its somatic versus dendritic location: perisomatic conductances could play a major role in maintaining action potential discharge and dendritic conductances would be more involved in other computational properties, such as those involving remote synaptic processing or bistable events.
Idoux
Erwin
E
Laboratoire de Neurobiologie des Réseaux Sensorimoteurs, UMR 7060, Université Paris Descartes (Paris 5) CNRS, 45 rue des Saints-Pères, 75270 Paris Cedex 06, France.
Eugène
Daniel
D
Chambaz
Antoine
A
Magnani
Christophe
C
White
John A
JA
Moore
Lee E
LE
D000818:Animals; D003712:Dendrites / Q000502:physiology* / Q000528:radiation effects; D004307:Dose-Response Relationship, Radiation; D004558:Electric Stimulation / Q000379:methods; D018691:Excitatory Amino Acid Antagonists / Q000494:pharmacology; D066298:In Vitro Techniques; D015640:Ion Channel Gating / Q000187:drug effects / Q000502:physiology / Q000528:radiation effects; D008526:Medulla Oblongata / Q000166:cytology; D008564:Membrane Potentials / Q000502:physiology* / Q000528:radiation effects; D008959:Models, Neurological; D009431:Neural Conduction / Q000502:physiology* / Q000528:radiation effects; D009474:Neurons / Q000145:classification / Q000166:cytology*; D018408:Patch-Clamp Techniques; D051381:Rats; D017208:Rats, Wistar; D019782:Riluzole / Q000494:pharmacology
D018691:Excitatory Amino Acid Antagonists; D019782:Riluzole