title: New diterpenoid alkaloids from Aconitum heterophyllum Wall: Selective butyrylcholinestrase inhibitors.
abstract: Two new diterpenoid alkaloids, heterophyllinine-A (1) and heterophyllinine-B (2), along with two known alkaloids dihydroatisine (3) and lycoctonine (4) were isolated from the roots of Aconitum heterophyllum Wall. The structure of (1) and (2), were deduced on the basis of spectral data. Compounds 1-2 inhibited acetylcholinesterase (AChE, EC 3.1.1.7) and butyrylcholinesterase (BChE, EC 3.1.1.8) enzymes in a concentration-dependent manner with percent inhibition ranging between 4.24% and 6.94 % and 79.1 % and 82.75 % for AChE and BChE, respectively indicating that compounds 1 and 2 are about thirteen times more specific to BChE than AChE.
authors.affiliation: Institute of Chemical Sciences and Department of Pharmacy, University of Peshawar, Peshawar, Pakistan. akhund_54@yahoo.co.uk
authors.forename: Muhammad
authors.initials: M
authors.lastname: Nisar
authors.forename: Manzoor
authors.initials: M
authors.lastname: Ahmad
authors.forename: Noreen
authors.initials: N
authors.lastname: Wadood
authors.forename: Muhammad Arif
authors.initials: MA
authors.lastname: Lodhi
authors.forename: Farzana
authors.initials: F
authors.lastname: Shaheen
authors.forename: M Iqbal
authors.initials: MI
authors.lastname: Choudhary
mesh_terms: D000110:Acetylcholinesterase / Q000187:drug effects; D000157:Aconitine / Q000031:analogs & derivatives; D031841:Aconitum / Q000737:chemistry*; D000470:Alkaloids / Q000302:isolation & purification* / Q000494:pharmacology; D000880:Anthraquinones; D002091:Butyrylcholinesterase / Q000187:drug effects*; D002800:Cholinesterase Inhibitors / Q000302:isolation & purification* / Q000494:pharmacology; D004224:Diterpenes / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D018517:Plant Roots / Q000737:chemistry
chemical_list: D000470:Alkaloids; D000880:Anthraquinones; D002800:Cholinesterase Inhibitors; D004224:Diterpenes; C511570:heterophylline; C539171:heterophyllinine A; C539172:heterophyllinine B; C093992:lycoctonine; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D000157:Aconitine