Changes in transient receptor potential vanilloid (TRPV) 1, 2, 3 and 4 expression in mouse inner ear following gentamicin challenge.
CONCLUSIONS
It is suggested that transient receptor potential vanilloid (TRPV)-1 and -2 may be of pathological significance for sensory cells and ganglions, while TRPV-3 and -4 may play an important part in neuroprotection of the inner ear.


OBJECTIVE
Changes in the expression of TRPV-1, -2, -3, and -4 in gentamicin (GM)-treated mouse inner ear were studied.


METHODS
CBA/J mice were used in this study. The localization of TRPV-1, -2, -3, and -4 in the inner ear of both untreated and GM-treated CBA/J animals (intratympanic injection of 5 mg GM) was investigated by immunohistochemistry.


RESULTS
TRPV-1, -2, and -3 were co-expressed in the inner ear sensory and ganglion cells, while TRPV-4 was also expressed in the stria vascularis and vestibular dark cells. Following GM treatment, the intensity of immunofluorescent reaction to TRPV-1 and TRPV-2 increased, while that to TRPV-3 and TRPV-4 decreased.
Ishibashi
Takuya
T
Department of Otolaryngology, Faculty of Medicine, Hiroshima University, Hiroshima, Japan.
Takumida
Masaya
M
Akagi
Nana
N
Hirakawa
Katsuhiro
K
Anniko
Matti
M
D000447:Aldehydes / Q000032:analysis; D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D019208:Brain-Derived Neurotrophic Factor / Q000032:analysis; D015220:Calcium Channels / Q000032:analysis*; D007758:Ear, Inner / Q000187:drug effects* / Q000473:pathology; D005727:Ganglia, Spinal / Q000187:drug effects / Q000473:pathology; D005839:Gentamicins / Q000633:toxicity*; D006199:Hair Cells, Auditory, Inner / Q000187:drug effects / Q000473:pathology; D018072:Hair Cells, Auditory, Outer / Q000187:drug effects / Q000473:pathology; D051379:Mice; D008808:Mice, Inbred CBA; D008856:Microscopy, Fluorescence; D009412:Nerve Fibers / Q000187:drug effects / Q000473:pathology; D009925:Organ of Corti / Q000187:drug effects / Q000473:pathology; D054776:Semicircular Ducts / Q000187:drug effects / Q000473:pathology; D011984:Sensory Receptor Cells / Q000187:drug effects / Q000473:pathology; D013136:Spiral Ganglion / Q000187:drug effects / Q000473:pathology; D013316:Stria Vascularis / Q000187:drug effects / Q000473:pathology; D050916:TRPV Cation Channels / Q000032:analysis*; D012668:Trigeminal Ganglion / Q000187:drug effects / Q000473:pathology; D014443:Tyrosine / Q000031:analogs & derivatives / Q000032:analysis; D014725:Vestibular Nerve / Q000187:drug effects / Q000473:pathology
D000447:Aldehydes; D000900:Anti-Bacterial Agents; D019208:Brain-Derived Neurotrophic Factor; D015220:Calcium Channels; D005839:Gentamicins; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse; C485717:Trpv2 protein, mouse; C486760:Trpv3 protein, mouse; C486576:Trpv4 protein, mouse; C002744:3-nitrotyrosine; D014443:Tyrosine; C027576:4-hydroxy-2-nonenal