[Development of chitosan/polyethylene glycols-succinate mitomycin C film drug delivery system and releasing character in vitro].
OBJECTIVE
To develop the chitosan/polyethylene glycols succinate (CH/PEG-SA) mitomycin C (MMC) film drug delivery system and its release effect in vitro.


METHODS
MMC loading in composite films was determined using a UV-visible spectrophotometer. Freeze-dried films (90 mg) were immersed in 1 mL PBS buffer (pH 7.4). The concentrations of MMC releasing in vitro were calculated refer to the standard curve of relationship between the concentrations of MMC and the value of UV-visible spectrophotometer. The curve of the concentrations of MMC releasing from the films in vitro was drawn at different time. The relationship between the films, structure and the drug releasing was revealed.


RESULTS
The films showed swelling without brittleness. The equation of Linear Regression was y=0.593x(3)-2.563x(2)+25.944x-0.236 (R2=1.000). The film had a good drug delivery capability. The samples weighing 20 mg were soaked into the liquid of PBS, the releasing concentrations of MMC were 14.9616 microg/mL at 12 days, 14.4824 microg/mL at 18 days and 11.4092 microg/mL at 32 days, which was higher than ID50 of MMC (10.4713 microg/L) to fibroblast. Then MMC was released at a low concentration. The releasing concentrations of MMC was 0.1793 microg/mL at 60 days until being delivered completely.


CONCLUSIONS
The flexibility is enhanced , and the mechanical function is improved, so that there is better nature of membrane. The initial burst is avoided more effectively, and the drug releasing would be maintained for a certain time.
Zhang
Yu
Y
Department of Orthopedic Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou Guangdong, 510282, P.R. China.
Zhou
Chusong
C
Jiang
Gangbiao
G
Yu
Bo
B
Gao
Fei
F
Fu
Dong
D
D048271:Chitosan / Q000008:administration & dosage*; D016503:Drug Delivery Systems; D066298:In Vitro Techniques; D016685:Mitomycin / Q000008:administration & dosage*; D011092:Polyethylene Glycols / Q000008:administration & dosage*
C119690:chitosan succinate; D011092:Polyethylene Glycols; D016685:Mitomycin; D048271:Chitosan