[Experimental study on effect of Liuwei Dihuang Pill on HSC from mouse marrow].
OBJECTIVE
To investigate the effect of Liuwei Dihuang Pill on the number, the surface marker, cell cycle and colony formation of HSC from mouse marrow.


METHODS
Old Kunming mice were randomly divided into 4 groups: the control group, low, middle and high dose of Liuwei Dihuang Pill group. Then we separated the HSC from marrow after 7 days fed with saline or Liywei Dihuang Pill respectively, numerated monocyte, detected the surface marker and cell cycle of the HSC by FACS and tested the colony forming by semisolid media culture.


RESULTS
Among the four groups, there was no obvious difference in the number of MNC, suspended cell and colony. The expression of Sca-1 and CD34 increased in the low and middle dosage group, it meant that the number of HSC elevated by low and middle dosage medicine. The ability of cell proliferation was also higher in the three dosage groups.


CONCLUSIONS
Liuwei Dihuang Pill activates HSC by increasing the number, proliferation and function of more primitive HSC.
Gao
Dongu
D
Fujian College of TCM, Fuzhou 350109, China.
Zheng
Liang-Pu
LP
Lin
Jiu-Mao
JM
Lin
Wei
W
Chen
Xu-Zheng
XZ
Song
Jun
J
Chen
Ke-Ji
KJ
D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D000067696:Ataxin-1; D000067528:Ataxins; D001854:Bone Marrow Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003114:Colony-Forming Units Assay; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D019650:Hematopoietic Stem Cell Mobilization / Q000379:methods; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D010946:Plants, Medicinal / Q000737:chemistry; D011897:Random Allocation; D013154:Spleen / Q000166:cytology / Q000187:drug effects
D018952:Antigens, CD34; D000067696:Ataxin-1; D000067528:Ataxins; C000593750:Atxn1 protein, mouse; D004365:Drugs, Chinese Herbal; C042579:Liuwei Dihuang Decoction; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins