A real-time polymerase chain reaction for the detection of Streptococcus pneumoniae in blood using a mouse model: a potential new "gold standard".
Better diagnostics for pneumococcal disease are urgently needed. In a murine model, real-time polymerase chain reaction was superior to conventional culture in detecting pneumococcus in blood, particularly in early disease and after antibiotic administration, and could distinguish between commensalism and infection.
Rouphael
Nadine G
NG
Meningitis and Vaccine-Preventable Diseases Branch, Immunology Laboratories, Division of Bacterial Diseases, Centers for Diseases Control and Prevention, Atlanta, GA 30333, USA. nadine.rouphael@gmail.com
Atwell-Melnick
Nikkol
N
Longo
Dana
D
Whaley
Melissa
M
Carlone
George M
GM
Sampson
Jackie S
JS
Ades
Edwin W
EW
D000818:Animals; D000900:Anti-Bacterial Agents / Q000627:therapeutic use; D016470:Bacteremia / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D001769:Blood / Q000382:microbiology; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D004195:Disease Models, Animal; D051379:Mice; D011008:Pneumococcal Infections / Q000175:diagnosis* / Q000188:drug therapy / Q000382:microbiology; D016133:Polymerase Chain Reaction / Q000379:methods*; D012680:Sensitivity and Specificity; D013296:Streptococcus pneumoniae / Q000235:genetics / Q000302:isolation & purification*; D013997:Time Factors
D000900:Anti-Bacterial Agents; D003470:Culture Media; D004269:DNA, Bacterial