title: 1,2,3,4,6-penta-O-galloyl-beta-D-glucose from Galla Rhois protects primary rat hepatocytes from necrosis and apoptosis.
abstract: In this study, we investigated the hepatoprotective effects of four compounds from Galla Rhois [gallic acid methyl ester, gallic acid, an equilibrium mixture of 3-galloyl-gallic acid and 4-galloyl-gallic acid isomers, and 1,2,3,4,6-penta- O-galloyl- beta- D-glucose (PGG)] in primary rat hepatocytes undergoing necrosis or apoptosis. Treatment with gallic acid methyl ester (12.5 and 50 microM) or PGG (3.125, 12.5 and 50 microM) reduced hepatocyte necrosis induced by tert-butyl hydroperoxide. PGG treatment (4 and 20 microM) also altered hepatocyte apoptosis induced by glycochenodeoxycholic acid. Based on these results, we propose that PGG warrants further evaluation as a hepatoprotective agent, because it protected primary rat hepatocytes from both necrosis and apoptosis.
authors.affiliation: Institute of Pharmaceutical Research and Development, Department of Pharmacy, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Eun-Jeon
authors.initials: EJ
authors.lastname: Park
authors.forename: Yu-Zhe
authors.initials: YZ
authors.lastname: Zhao
authors.forename: Ren-Bo
authors.initials: RB
authors.lastname: An
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.forename: Dong Hwan
authors.initials: DH
authors.lastname: Sohn
mesh_terms: D000818:Animals; D001042:Aphids; D017209:Apoptosis / Q000187:drug effects*; D002478:Cells, Cultured; D005707:Gallic Acid / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use; D022781:Hepatocytes / Q000187:drug effects*; D047348:Hydrolyzable Tannins / Q000302:isolation & purification / Q000494:pharmacology* / Q000627:therapeutic use; D009336:Necrosis / Q000517:prevention & control; D010941:Plant Tumors; D051381:Rats; D029045:Rhus / Q000737:chemistry
chemical_list: D047348:Hydrolyzable Tannins; C435084:pentagalloylglucose; C052082:methyl gallate; D005707:Gallic Acid