Selective separation of amino acids with a charged inorganic nanofiltration membrane: effect of physicochemical parameters on selectivity.
A charged organic-inorganic nanofiltration (NF) membrane prototype was used to separate a mixture of nine amino acids (AA) on the basis of differential electrostatic interactions with the membrane because, for a given pH, some of them were positively charged, some were negative, and some were zwitterions. Effect of pH, amino acid concentration (C(r)), and added ionic strength ([NaCI]) on the process selectivity was studied. A global statistical study revealed that pH was the dominant parameter regarding fractionation. C(r) and [NaCI] had a weaker effect, but the ratio C(r)/[NaCI] demonstrated a pronounced effect on system selectivity. Two split-ups of the mixture were obtained at pH 2 and at pH 12, for a 1-g/L total AA concentration and a C(r)/[NaCI] ratio of 0.16. Under these conditions, the differences in transmissions between basic and acid AA were higher than 70%. Interpretation of the results according to the Donnan theory allows us to foresee the potentialities of charged nanofiltration membranes for the fractionation of a complex mixture, such as peptidic hydrolysate to streams containing peptides and amino acids having different isoelectric points. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 291-302, 1997.
Garem
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INRA, Laboratoire de Recherches de Technologie Laitière, 65 rue de Saint-Brieuc, 35042 Rennes Cedex, France. agarem@/labtechno.roazhon.inra.fr
Daufin
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Maubois
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Léonil
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