Effect of human papilloma virus expression on clinical course of laryngeal papilloma.
CONCLUSIONS
Our observations suggest that human papilloma virus (HPV) 6/11 is the main causative agent of laryngeal papilloma and that detection of active HPV DNA expression may be helpful in identifying patients with aggressive recurrent laryngeal papilloma.


OBJECTIVE
HPV is assumed to be the main causative agent of this disease. We investigated the expression of the entire genotype of HPV in cases of laryngeal papilloma and correlated their expression with the clinical course of the disease.


METHODS
Seventy cases of laryngeal papilloma were evaluated for the presence of the HPV genome by in situ hybridization (ISH) using wide-spectrum HPV DNA probe. Specific types of HPV infection were determined by DNA ISH using type-specific HPV DNA probes (HPV 6, 11, 16, 18, 31, 33). Separate analyses were conducted comparing viral types, frequency of recurrences and duration of disease-free periods.


RESULTS
We detected HPV DNA in 40 of the 70 laryngeal papilloma cases (57%). In particular, HPV DNA was detected in 75% of the juvenile types. There were significant associations between HPV and laryngeal papilloma (p<0.01). Among the HPV-positive cases, major specific types were HPV 6/11 (97%). Significant associations were also noted between viral expression and clinical course.
Kim
Kwang Moon
KM
Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
Cho
Nam Hoon
NH
Choi
Hong Shik
HS
Kim
Young Ho
YH
Byeon
Hyung Kwon
HK
Min
Hyun Jin
HJ
Kim
Se-Heon
SH
D000328:Adult; D002648:Child; D015342:DNA Probes; D004279:DNA, Viral / Q000235:genetics; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D017403:In Situ Hybridization; D007822:Laryngeal Neoplasms / Q000401:mortality / Q000473:pathology / Q000821:virology*; D008297:Male; D009364:Neoplasm Recurrence, Local / Q000821:virology; D010212:Papilloma / Q000401:mortality / Q000473:pathology / Q000821:virology*; D027383:Papillomaviridae / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000821:virology*; D012074:Remission Induction; D012189:Retrospective Studies
D015342:DNA Probes; D004279:DNA, Viral