Yeast Barcoders: a chemogenomic application of a universal donor-strain collection carrying bar-code identifiers.
The ability to perform complex bioassays in parallel enables experiments that are otherwise impossible because of throughput and cost constraints. For example, highly parallel chemical-genetic screens using pooled collections of thousands of defined Saccharomyces cerevisiae gene deletion strains are feasible because each strain is bar-coded with unique DNA sequences. It is, however, time-consuming and expensive to individually bar-code individual strains. To provide a simple and general method of barcoding yeast collections, we built a set of donor strains, called Barcoders, with unique bar codes that can be systematically transferred to any S. cerevisiae collection. We applied this technology by generating a collection of bar-coded 'decreased abundance by mRNA perturbation' (DAmP) loss-of-function strains comprising 87.1% of all essential yeast genes. These experiments validate both the Barcoders and the DAmP strain collection as useful tools for genome-wide chemical-genetic assays.
Yan
Zhun
Z
Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 160 College Street, Toronto, Ontario M5S 3E1, Canada.
Costanzo
Michael
M
Heisler
Lawrence E
LE
Paw
Jadine
J
Kaper
Fiona
F
Andrews
Brenda J
BJ
Boone
Charles
C
Giaever
Guri
G
Nislow
Corey
C
D000483:Alleles; D001681:Biological Assay / Q000379:methods*; D001330:Electronic Data Processing / Q000379:methods*; D017353:Gene Deletion; D005821:Genetic Techniques; D016681:Genome, Fungal / Q000235:genetics*; D006579:Heterozygote; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015203:Reproducibility of Results; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000145:classification* / Q000187:drug effects / Q000235:genetics*; D012680:Sensitivity and Specificity
D012331:RNA, Fungal; D012333:RNA, Messenger