Biological response of human bone marrow stromal cells to sandblasted titanium nitride-coated implant surfaces.
Titanium nitride (TiN) coating has been proposed as an adjunctive surface treatment aimed to increase the physico-mechanical and aesthetic properties of dental implants. In this study we investigated the biological response of primary human bone marrow stromal cells (BMSC) to TiN-coated sandblasted (TiN-SB) compared to uncoated sandblasted (SB) surfaces. SB and TiN-SB disks were qualitatively and quantitatively analyzed by atomic force microscopy. BMSC were obtained from healthy donors and their adhesion and proliferation on the titanium disks were evaluated by scanning electron microscopy and viability assay. The osteoblastic differentiation, in terms of alkaline phosphatase activity, osteocalcin synthesis, and extracellular mineralization, was assessed by specific immunoenzymatic or spectrophotometric assays. No difference (P > 0.05) between TiN-SB and SB disks was found in terms of any of the investigated parameters. TiN-coating showed to maintain the topographical characteristics of sandblasted titanium surfaces and their biological affinity toward bone precursors.
Annunziata
Marco
M
Department of Odontostomatological, Orthodontic and Surgical Disciplines, Second University of Naples, Via L. De Crecchio, 6, 80138, Naples, Italy.
Guida
Luigi
L
Perillo
Letizia
L
Aversa
Raffaella
R
Passaro
Irene
I
Oliva
Adriana
A
D000328:Adult; D000469:Alkaline Phosphatase / Q000032:analysis; D001854:Bone Marrow Cells / Q000166:cytology; D002113:Calcification, Physiologic; D002448:Cell Adhesion; D049109:Cell Proliferation; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003722:Dental Alloys / Q000737:chemistry*; D015921:Dental Implants; D005109:Extracellular Matrix / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D010006:Osteoblasts / Q000166:cytology / Q000648:ultrastructure; D015675:Osteocalcin / Q000096:biosynthesis; D017154:Stromal Cells / Q000166:cytology* / Q000648:ultrastructure; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry*
D020099:Coated Materials, Biocompatible; D003722:Dental Alloys; D015921:Dental Implants; D015675:Osteocalcin; C041500:titanium nitride; D014025:Titanium; D000469:Alkaline Phosphatase