Some biological and physicochemical properties of blue-green algal virus LPP-1.
Adsorption and one-step growth experiments performed with blue-green algal virus (BGAV) LPP-1 under normal conditions revealed a slow adsorption rate, in comparison with bacteriophage systems, followed by a 7-hour latent period. The burst size was approximately 100 virus particles per cell. The molecular weight of the virus obtained from sedimentation and diffusion measurements was 51 +/- 3 x 10(6) daltons, and the hydration was calculated to be 0.37 g of H2O per gram of virus. Phosphorus analysis indicated a DNA content of 40% w/w, while amino acid analysis revealed all the common amino acids to be present. At reduced magnesium concentrations, the virus breaks apart, liberating its DNA, which then can be separated from the protein by sedimentation in a CsCl density gradient. A protein component was obtained as ghosts. Neutralization experiments with BGAV antiserum established no relationship to bacteriophages T1, T2, or T3.
Goldstein
D A
DA
Department of Biophysics, University of Pittsburgh, Pittsburgh, Pennsylvania 15213, USA.
Bendet
I J
IJ
Lauffer
M A
MA
Smith
K M
KM