title: Eckol protects V79-4 lung fibroblast cells against gamma-ray radiation-induced apoptosis via the scavenging of reactive oxygen species and inhibiting of the c-Jun NH(2)-terminal kinase pathway.
abstract: The radioprotective effect of eckol against gamma-ray radiation-induced oxidative stress and its possible protective mechanisms were investigated. Eckol was found to reduce the intracellular reactive oxygen species generated by gamma-ray radiation. Moreover, eckol also protected against radiation-induced cellular DNA damage and membrane lipid peroxidation, which are the main targets of radiation-induced damage. In addition, eckol recovered the cell viability damaged by radiation via the inhibition of apoptosis. Irradiated cells with eckol treatment reduced the expression of bax, the activation of caspase 9 and caspase 3, which were induced by radiation. However, irradiated cells with eckol recovered the expression of bcl-2 and mitochondrial cytochrome c which were decreased by radiation. The anti-apoptotic effect of eckol exerted via the inhibition of mitogen-activated protein kinase kinase-4 (MKK4/SEK1)-c-Jun NH(2)-terminal kinase (JNK)-activator protein 1 (AP-1) cascades induced by radiation. In summary, the results suggest that eckol protects cells against the oxidative stress induced by radiation via the reduction of reactive oxygen species and the attenuation of activation in SEK1-JNK-AP-1 pathway.
authors.affiliation: Department of Biochemistry, College of Medicine and Applied Radiological Science Research Institute, Cheju National University, Jeju-si 690-756, Republic of Korea.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhang
authors.forename: Kyoung Ah
authors.initials: KA
authors.lastname: Kang
authors.forename: Mei Jing
authors.initials: MJ
authors.lastname: Piao
authors.forename: Dong Ok
authors.initials: DO
authors.lastname: Ko
authors.forename: Zhi Hong
authors.initials: ZH
authors.lastname: Wang
authors.forename: In Kyung
authors.initials: IK
authors.lastname: Lee
authors.forename: Bum Joon
authors.initials: BJ
authors.lastname: Kim
authors.forename: Il Yun
authors.initials: IY
authors.lastname: Jeong
authors.forename: Taekyun
authors.initials: T
authors.lastname: Shin
authors.forename: Jae Woo
authors.initials: JW
authors.lastname: Park
authors.forename: Nam Ho
authors.initials: NH
authors.lastname: Lee
authors.forename: Jin Won
authors.initials: JW
authors.lastname: Hyun
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects / Q000528:radiation effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D006224:Cricetinae; D003412:Cricetulus; D004249:DNA Damage / Q000187:drug effects / Q000528:radiation effects; D004147:Dioxins / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects / Q000528:radiation effects; D016166:Free Radical Scavengers / Q000494:pharmacology; D005720:Gamma Rays / Q000009:adverse effects*; D048031:JNK Mitogen-Activated Protein Kinases / Q000378:metabolism; D008168:Lung / Q000166:cytology / Q000187:drug effects / Q000528:radiation effects; D048670:MAP Kinase Kinase 4 / Q000187:drug effects / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D017382:Reactive Oxygen Species / Q000378:metabolism*; D018808:Transcription Factor AP-1 / Q000187:drug effects / Q000528:radiation effects
chemical_list: D004147:Dioxins; D016166:Free Radical Scavengers; D017382:Reactive Oxygen Species; D018808:Transcription Factor AP-1; C060311:eckol; D048031:JNK Mitogen-Activated Protein Kinases; D048670:MAP Kinase Kinase 4