title: Effects of estrogens on proliferation and differentiation of neural stem/progenitor cells.
abstract: We investigated the effect of the female hormone 17beta-estradiol (E2) and the hormone mimic bisphenol A (BPA) on the proliferation and differentiation of rat neural stem/progenitors cells (NS/PCs) cultured from the telencephalon of embryonic day-15 rats. Basic fibroblast growth factor (FGF-2) is a potent mitogen of early generated NS/PCs, and is used for the proliferation of NS/PCs in vitro. Administration of E2 or BPA alone to the NS/PCs stimulated their proliferation in the absence but not in the presence of FGF-2. E2- or BPA-treatment increased the ratio of the oligodendrocytes generated from the NS/PCs to total cells; however, this ratio did not change when the cells were stimulated with platelet-derived growth factor (PDGF), a mitogen for oligodendrocyte precursors, or with neurotrophin-3, an oligogenic factor for glial progenitor cells. These results suggest that estrogens would influence the fate of NS/PCs when the cells are poorly supplied with mitogens or differentiation factors during the early stages of neurogenesis.
authors.affiliation: Laboratory of Molecular Biology, Gifu Pharmaceutical University, Gifu, Japan.
authors.forename: Makiko
authors.initials: M
authors.lastname: Okada
authors.forename: Koichi
authors.initials: K
authors.lastname: Murase
authors.forename: Akihisa
authors.initials: A
authors.lastname: Makino
authors.forename: Mitsunari
authors.initials: M
authors.lastname: Nakajima
authors.forename: Teppei
authors.initials: T
authors.lastname: Kaku
authors.forename: Shoei
authors.initials: S
authors.lastname: Furukawa
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Furukawa
mesh_terms: D000818:Animals; D001483:Base Sequence; D001559:Benzhydryl Compounds; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D017931:DNA Primers; D004958:Estradiol / Q000494:pharmacology*; D007150:Immunohistochemistry; D010636:Phenols / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D013687:Telencephalon / Q000166:cytology / Q000187:drug effects*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D017931:DNA Primers; D004958:Estradiol; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds