A novel method for study of gastric mechanical functions in conscious mice.
A novel method has been developed for simultaneous study of gastric emptying, antral motility, and gastric muscle tone in conscious mice. Intragastric pressure was measured during infusion of an X-ray-opaque, viscous meal through a chronically implanted gastric fistula (0.25 ml/min). Compared with vehicle treatment, molsidomine (nitric oxide donor) and atropine (muscarinic receptor antagonist) treatment significantly reduced the area under the intragastric pressure curve (AUC) by 37 +/- 4% and 35 +/- 3%, respectively, (mean +/- S.E.M.) whereas N (G)-nitro-L-arginine methyl ester (L-NAME; nitric oxide synthase inhibitor) significantly increased the AUC by 20 +/- 3%. Atropine also significantly reduced the frequency and amplitude of stomach contraction-induced intragastric pressure waves while molsidomine only reduced the frequency. Gastric emptying, as assessed by X-ray imaging, was significantly delayed after L-NAME and atropine treatment. This methodology is the first to enable simultaneous assessment of gastric emptying, antral motility, and gastric tone in conscious mice and confirmed the important role of nitrergic and cholinergic innervation.
Janssen
Pieter
P
AstraZeneca R&D Mölndal, Integrative Pharmacology, Gastrointestinal Biology, Molndal, Sweden. Pieter.Janssen@Novartis.com
Nielsen
Maria Astin
MA
Hirsch
Ika
I
Gillberg
Per-Göran
PG
Hultin
Leif
L
D000818:Animals; D001285:Atropine / Q000494:pharmacology; D005746:Gastric Emptying / Q000187:drug effects*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008981:Molsidomine / Q000494:pharmacology; D018727:Muscarinic Antagonists / Q000494:pharmacology; D009129:Muscle Tonus / Q000187:drug effects*; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D020030:Nitric Oxide Donors / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D013270:Stomach / Q000187:drug effects*
D018727:Muscarinic Antagonists; D020030:Nitric Oxide Donors; D001285:Atropine; D008981:Molsidomine; D019001:Nitric Oxide Synthase; D019331:NG-Nitroarginine Methyl Ester