Further studies on the association of herpes simplex virus type 1 DNA with host DNA during productive infection.
Our previous finding of an association between herpes simplex virus type 1 (HSV-1) and African green monkey kidney (BSC-1) DNAs in productively infected cells has been extended by investigating the influence of the time course, temperature, and multiplicity of infection on the extent of this association. The interaction of host and viral DNAs was not only dependent on the concentration of intracellular viral DNA, but also upon the particular conditions of infection. An examination of the time course of infection revealed that the amount of free viral DNA per cell reached a maximum well before the amount of viral-host-associated DNA did so, while an examination of the effect of temperature revealed a threefold variation in the amount of viral-host-associated DNA over a temperature range (33.5-39.5 degrees ) in which the amount of free viral DNA was essentially constant. The quantity of HSV-1 DNA associated with host-cell DNA ranges from about 100 to 1500 genome equivalents per cell, depending on the conditions of infection, and it appears that between 5 and 40% of the association is alkali stable.
Biegeleisen
K
K
Department of Biochemistry, New York University School of Medicine, 550 First Avenue, New York, New York 10016, USA.
Yanagi
K
K
Rush
M G
MG