Perspectives in paramagnetic NMR of metalloproteins.
NMR experiments and tools for the characterization of the structure and dynamics of paramagnetic proteins are presented here. The focus is on the importance of (13)C direct-detection NMR for the assignment of paramagnetic systems in solution, on the information contained in paramagnetic effects observed both in solution and in the solid state, and on novel paramagnetism-based tools for the investigation of conformational heterogeneity in protein-protein complexes or in multi-domain proteins.
Bertini
Ivano
I
Magnetic Resonance Center, University of Florence, Via L. Sacconi 6, 50019, Sesto Fiorentino, (FI), Italy. ivanobertini@cerm.unifi.it
Luchinat
Claudio
C
Parigi
Giacomo
G
Pierattelli
Roberta
R
D002247:Carbon Isotopes; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008667:Metalloproteins / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D012996:Solutions / Q000737:chemistry
D002247:Carbon Isotopes; D008667:Metalloproteins; D012996:Solutions