Juice of eclipta prostrata inhibits cell migration in vitro and exhibits anti-angiogenic activity in vivo.
BACKGROUND
The invasion of cancer cells is critical for metastasis. The effects of Eclipta prostrata, a Thai medicinal plant, on invasion, migration and adhesion of cancer cells were investigated and the anti-angiogenic activity in vivo was evaluated.


METHODS
In vitro invasion and migration assays were performed in modified Boyden chambers. In vivo anti-angiogenic activity was determined using the chick chorioallantoic membrane (CAM) assay.


RESULTS
E. prostrata juice inhibited cancer invasion and migration, without affecting cell adhesion. Cell migration was inhibited in a variety of cancer cell types and in endothelial cells, with IC50 values of 31-70 microg/ml, much lower than the IC50 values for cytotoxicity of 203-1,217 microg/ml for cancer cells and >4,000 microg/ml for endothelial cells. Fifty percent inhibition of angiogenesis by E. prostrata juice was observed at 200 microg/egg.


CONCLUSIONS
E. prostrata juice inhibited cancer and endothelial cell migration in vitro and also showed in vivo anti-angiogenic activity.
Lirdprapamongkol
Kriengsak
K
Laboratory of Biochemistry, Chulabhorn Research Institute, Bangkok, Thailand.
Kramb
Jan-Peter
JP
Chokchaichamnankit
Daranee
D
Srisomsap
Chantragan
C
Surarit
Rudee
R
Sila-Asna
Monnipha
M
Bunyaratvej
Ahnond
A
Dannhardt
Gerd
G
Svasti
Jisnuson
J
D020533:Angiogenesis Inhibitors / Q000494:pharmacology*; D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D002642:Chick Embryo; D036601:Eclipta / Q000737:chemistry*; D042783:Endothelial Cells / Q000187:drug effects; D006801:Humans; D009369:Neoplasms / Q000473:pathology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology
D020533:Angiogenesis Inhibitors; D010936:Plant Extracts