title: Action of lipolytical enzymes in biphasic organic-aqueous systems: dynamics of the irreversible Michaelis-Menten reaction.
abstract: Through simple model analysis, the mass action kinetic model for lipolytic enzymes in biphasic aqueous-organic systems can be simplified using the quasi-steady state assumption (or the quasi-equilibrium state assumption) for the adsorbed enzyme E* or the enzyme-substrate complex E*S. Some parameter combinations leading to the above assumptions are derived confirmed by full numerical integration of the whole enzymatic process. The results may be classified into three categories: (1) the quasi-equilibrium state assumption for E*, (2) the quasi-steady state assumption for E*, and (3) the quasi-steady state assumption for E*S. Further simplification for both E* and E*S is also discussed.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan, Republic of China.
authors.forename: S W
authors.initials: SW
authors.lastname: Tsai
authors.forename: H J
authors.initials: HJ
authors.lastname: Wei
authors.forename: C L
authors.initials: CL
authors.lastname: Chiang