Glutaraldehyde fixation of central nervous tissue: an electron microscopic evaluation in the isolated rabbit retina.
The isolated rabbit retina was studied electron microscopically after fixation with a 3% solution of glutaraldehyde in a 0.05 M Sørensen's phosphate buffer. In radial sections, the inner segments, nuclei, and synapses of the photoreceptor cells seemed similar in size to those from retinas that had been fixed in an isotonic solution containing 1 % crystalline osmium tetroxide in the incubating medium used for the isolation procedure. However, when the number of comparable structures was greatly increased by viewing them in tangential sections, the cellular shrinkage and mitochondrial swelling produced by this widely used, hypertonic, glutaraldehyde fixative were obvious.
Webster
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HD
Department of Neurology, Bascom Palmer Eye Institute, University of Miami School of Medicine, Mixter Laboratories for Neurosurgical Research in the Massachusetts General Hospital, Departments of Neurology and Biochemistry, Harvard Medical School, USA.
Ames
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