title: A reagentless amperometric electrode based on carbon paste, chemically modified with D-lactate dehydrogenase, NAD(+), and mediator containing polymer for D-lactic acid analysis. II. On-line monitoring of fermentation process.
abstract: The production of D-lactic acid by Lactobacillus delbrueckii (ATCC 9649) during fermentation was monitored on-line with a reagentless D-lactate dehydrogenase modified carbon paste electrode in a flow injection system integrated with a filtration sampling device. The time delay between sampling and detection was approximately 6 min. The use of an electropolymerized ortho-phenylenediamine membrane on the elctrode resulted in a very selective sensor response with acceptable stability and sensitivity. The D-lactate concentrations determined on-line agreed well with those determined by a standard method, suggesting that this sensor system is suitable for on-line monitoring of fermentation processes. (c) 1995 John Wiley & Sons, Inc.
authors.affiliation: Department of Biotechnology, Chemical Center, Lund University, S-221 00 Lund, Sweden.
authors.forename: H C
authors.initials: HC
authors.lastname: Shu
authors.forename: L
authors.initials: L
authors.lastname: Gorton
authors.forename: B
authors.initials: B
authors.lastname: Persson
authors.forename: B
authors.initials: B
authors.lastname: Mattiasson