title: Sporulation: an alternative way to recover recombinant proteins from Bacillus subtilis.
abstract: A recombinant strain of Bacillus subtilis engineered for endocellular expression of human interleukin-1 receptor antagonist (IL-Ira) was subjected to sporulation. The recombinant protein was recovered from the sporulation supernatant in quantities, purity, and activity comparable with those obtained from a traditional cell lysate. Thus, exploitation of this natural mechanism of autolysis could overcome problems of intact protein recovery related to the cell disruption step.
authors.affiliation: Consorzio Biolaq, Via Campo di Pile, I-67100 L'Aquila, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Maurizi
authors.forename: P
authors.initials: P
authors.lastname: Bossù
authors.forename: D
authors.initials: D
authors.lastname: Boraschi
authors.forename: E
authors.initials: E
authors.lastname: Ulisse
authors.forename: A
authors.initials: A
authors.lastname: Tagliabue
authors.forename: P
authors.initials: P
authors.lastname: Ruggiero