Transfection with baculovirus DNA.
Purified DNA from the nuclear polyhedrosis viruses of Autographa californica (AcM NPV) and Rachiplusia ou (RoMNPV) were found to be infectious in TN-368 cells employing the calcium phosphate precipitation technique (F. L. Graham and A. J. van der Eb, Virology, 52 ,456-467, 1973). Transfection with AcM NPV produced 3600 PFU/microg DNA compared to 2900 PFU/mug DNA with RoM NPV. Of eight baculovirus DNAs tested, only AcM NPV DNA and RoM NPV DNA could transfect TN-368 cells. The in vitro host range of AcM NPV DNA was determined to be the same as AcM NPV extracellular virus. AcM NPV Form I DNA was fourfold more infectious in TN-368 cells than Form II DNA.
Burand
J P
JP
Department of Entomology, Texas A&M University, College Station, Texas 77843, USA.
Summers
M D
MD
Smith
G E
GE