Sporulation: an alternative way to recover recombinant proteins from Bacillus subtilis.
A recombinant strain of Bacillus subtilis engineered for endocellular expression of human interleukin-1 receptor antagonist (IL-Ira) was subjected to sporulation. The recombinant protein was recovered from the sporulation supernatant in quantities, purity, and activity comparable with those obtained from a traditional cell lysate. Thus, exploitation of this natural mechanism of autolysis could overcome problems of intact protein recovery related to the cell disruption step.
Maurizi
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G
Consorzio Biolaq, Via Campo di Pile, I-67100 L'Aquila, Italy.
Bossù
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P
Boraschi
D
D
Ulisse
E
E
Tagliabue
A
A
Ruggiero
P
P