title: The effect of platelet-rich plasma on healing in critical-size long-bone defects.
abstract: The role of platelet-rich plasma (PRP) as a promoter of bone healing remains controversial. The hypothesis investigated was that PRP improves bone healing of a critical-size diaphyseal radius defect in a rabbit model. The bone defect was filled with a high-surface ceramic scaffold, calcium-deficient hydroxyapatite (CDHA), with the addition of allogenic PRP, mesenchymal stem cells (MSC) or both. PRP yielded better bone formation than the empty CDHA scaffold as determined by both histology and micro-computer tomography (p<0.05) after 16 weeks, whereas no difference was observed on biomechanical testing. Similar behavior was found in samples with MSC; however, the combination of MSC and PRP did not further improve bone healing. Furthermore, the resorption of CDHA was improved by the addition of PRP, MSC and MSC/PRP, but there were no differences between the groups. The areas of bone formation were greater in areas adjacent to the bone resection areas and towards the intact ulna. In conclusion, PRP improves bone healing in a diaphyseal rabbit model on CDHA and the combination of CDHA. This study supports the allogenic use of PRP for bone healing as an off-the-shelf therapy.
authors.affiliation: Orthopaedic Surgery Hospital, University of Heidelberg, Schlierbacher Landstrasse 200a, 69118 Heidelberg, Germany. Philip.Kasten@ok.uni-heidelberg.de
authors.forename: Philip
authors.initials: P
authors.lastname: Kasten
authors.forename: Julia
authors.initials: J
authors.lastname: Vogel
authors.forename: Florian
authors.initials: F
authors.lastname: Geiger
authors.forename: Philipp
authors.initials: P
authors.lastname: Niemeyer
authors.forename: Reto
authors.initials: R
authors.lastname: Luginbühl
authors.forename: Krisztian
authors.initials: K
authors.lastname: Szalay
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000378:metabolism; D001861:Bone Regeneration / Q000502:physiology*; D001842:Bone and Bones / Q000473:pathology / Q000502:physiology; D002516:Ceramics / Q000737:chemistry / Q000378:metabolism; D005260:Female; D006801:Humans; D006882:Hydroxyapatites / Q000737:chemistry / Q000378:metabolism; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000502:physiology; D016348:Osseointegration; D010012:Osteogenesis / Q000502:physiology; D053657:Platelet-Rich Plasma / Q000378:metabolism*; D011817:Rabbits; D023822:Tissue Engineering / Q000295:instrumentation / Q000379:methods; D054457:Tissue Scaffolds; D014184:Transplantation, Homologous; D014945:Wound Healing
chemical_list: D001672:Biocompatible Materials; D006882:Hydroxyapatites