title: Growth cycle of a virus, PBCV-1, that infects Chlorella-like algae.
abstract: The growth and purification of milligram quantities of a large double-stranded DNA virus, PBCV-1, which replicates in a Chlorella-like alga is described. The virus had an adsorption rate constant of ca. 5 x 10(-9) ml/min, a latent period of 150 to 180 min, and a burst size of 200 to 350 PFUs when the host was actively growing in the light. The eclipse period was 30 to 50 min shorter than the latent period. PBCV-1 also replicated in dark grown Chlorella but the burst size was reduced ca. 50%. The photosynthetic inhibitor, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, had no effect on viral replication. Thus viral replication does not require host photosynthesis. Viral infection rapidly inhibited both growth and CO2 fixation of the host Chlorella.
authors.affiliation: Department of Plant Pathology University of Nebraska, Lincoln, Nebraska, 68583, USA.
authors.forename: J L
authors.initials: JL
authors.lastname: Van Etten
authors.forename: D E
authors.initials: DE
authors.lastname: Burbank
authors.forename: Y
authors.initials: Y
authors.lastname: Xia
authors.forename: R H
authors.initials: RH
authors.lastname: Meints