Poly(gamma-L-diaminobutanoic acid), a novel poly(amino acid), coproduced with poly(epsilon-L-lysine) by two strains of Streptomyces celluloflavus.
Two poly(epsilon-L-lysine) (epsilon-PL) producer strains of Streptomyces celluloflavus secreted a novel polymeric substance into their culture broths along with epsilon-PL. Three types of HPLC analysis plus one- and two-dimensional 1H and 13C nuclear magnetic resonance experiments revealed that the secreted substance was poly(gamma-L-diaminobutanoic acid) (gamma-PAB), an L-alpha,gamma-diaminobutanoic acid (L-DAB) homopolymer linking between y-amino and alpha-carboxylic acid functional groups. The gamma-PABs from the two strains had an identical chemical structure, and the same number-average molecular weight of 2100-2200. No copolymers composed of the two amino acids L-DAB and L-lysine were found in either of the broths from the producers. Both strains coproduced high levels of the two poly(amino acid)s in the presence of SO4(2-) at pH 4.0 and 4.5 L min(-1) aeration in a 5-L jar fermentor. gamma-PAB exhibited strong inhibitory activities against various yeasts and weaker actions against bacteria than epsilon-PL. gamma-PAB may have various biological functions similar to epsilon-PL, and the use of gamma-PAB along with epsilon-PL would be advantageous for technical applications in various fields.
Takehara
Munenori
M
Department of Materials Science, University of Shiga Prefecture, Hikone, Japan.
Saimura
Masayuki
M
Inaba
Haruka
H
Hirohara
Hideo
H
D000596:Amino Acids / Q000737:chemistry* / Q000378:metabolism* / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005285:Fermentation; D008239:Lysine / Q000737:chemistry / Q000378:metabolism; D013302:Streptomyces / Q000737:chemistry* / Q000378:metabolism*; D015003:Yeasts / Q000187:drug effects
D000596:Amino Acids; D001704:Biopolymers; D008239:Lysine