Use of soybean protein hydrolysates for promoting proliferation of human keratinocytes in serum-free medium.
Human keratinocytes are generally cultured in media containing bovine pituitary extract (BPE), an animal product that can be a source of infectious contaminants. We investigated whether a safer plant product could replace BPE in the culture medium. Medium containing both BPE and soy protein hydrolysates (Bacto Soytone and Soy Hydrolysate) produced the largest number of viable cells, followed in descending order by medium supplemented only with BPE, only with the hydrolysates, and without supplementation (basal medium only). Soybean protein is thus an excellent source of nutrients for the growth of adherent keratinocytes, although they do not fully substitute for BPE.
Lee
Yong Kwon
YK
College of Life Sciences and Biotechnology, Korea University, Seoul, 136-713, South Korea.
Kim
Seung Yeul
SY
Kim
Ki Heon
KH
Chun
Bok-Hwan
BH
Lee
Kweon-Haeng
KH
Oh
Duk Jae
DJ
Chung
Namhyun
N
D049109:Cell Proliferation / Q000187:drug effects*; D016895:Culture Media, Serum-Free / Q000494:pharmacology; D004247:DNA / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D019344:Lactic Acid / Q000378:metabolism; D030262:Soybean Proteins / Q000378:metabolism / Q000494:pharmacology*
D016895:Culture Media, Serum-Free; D030262:Soybean Proteins; D019344:Lactic Acid; D004247:DNA; D005947:Glucose