Identification of C-terminal neighbours of amino acid residues without an aliphatic 13Cgamma as an aid to NMR assignments in proteins.
We propose a methodology that uses GFT (3,2)D CB(CACO)NNH experiment to rapidly collect the data and readily identify six amino acid residue types (Ala, Asn, Asp, Cys, Gly and Ser) in any given protein. Further, the experiment can distinguish the redox state of Cys residues. The proposed experiment in its two forms will have wide range of applications in resonance assignment strategies and structure determination of proteins.
Barnwal
Ravi Pratap
RP
Department of Chemical Sciences, Tata Institute of Fundamental Research, Colaba, Mumbai, India.
Rout
Ashok K
AK
Atreya
Hanudatta S
HS
Chary
Kandala V R
KV
D020816:Amino Acid Motifs / Q000502:physiology*; D002247:Carbon Isotopes / Q000737:chemistry; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D010363:Pattern Recognition, Automated / Q000379:methods*; D017434:Protein Structure, Tertiary / Q000502:physiology; D011506:Proteins / Q000737:chemistry*; D012680:Sensitivity and Specificity
D002247:Carbon Isotopes; D011506:Proteins