title: Formation of carotenoids by rhodotorula glutinis in whey ultrafiltrate.
abstract: The growth and carotenoid biosynthesis of the yeast Rhodotorula glutinis was studied by cocultivation with Lactobacillus helveticus in cheese ultrafiltrate containing 3.9% and 7.1% lactose. By growing this mixed culture in a 15-L fermentor MBR AG (Switzerland) at an air flow rate of 0.5 L/L min and agitation at 220 rpm for 6 days, a total yield of carotenoids of 268 mug/g dry cells wasobtained. Carotenoids were formed almost parallel with the cell growth, anda maximum production was reached at an early stationary phase. A high-performance liquid chromatographic system (HPLC) permitting simultaneous determination of major carotenoid pigments was used. The three main pigments (torularhodin, beta-carotene, and torulene) were formed in Rhodotorula glutinis, and reached a maximum concentration as follows: 182.0, 43.9, 23.0 mug,g dry cells. (c) 1994 John Wiley & Sons, Inc.
authors.affiliation: Laboratory of Applied Microbiology, Institute of Microbiology, Bulgarian Academy of Sciences, 4000 Plovdiv, 26 Maritza Blvd., Plovdiv, Bulgaria.
authors.forename: G
authors.initials: G
authors.lastname: Frengova
authors.forename: E
authors.initials: E
authors.lastname: Simova
authors.forename: K
authors.initials: K
authors.lastname: Pavlova
authors.forename: D
authors.initials: D
authors.lastname: Beshkova
authors.forename: D
authors.initials: D
authors.lastname: Grigorova