An amperometric horseradish peroxidase inhibition biosensor for the determination of phenylhydrazine.
An amperometric horseradish peroxidase (HRP) inhibition biosensor has been substantially constructed by the help of N,N-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS). The preparation steps and the biosensor response to phenylhydrazine were monitored by electrochemical impedance spectroscopy (EIS), cyclic voltammetry, and chronoamperometry. The proposed biosensor could be applied to determine phenylhydrazine in a 0.10 M phosphate buffer solution containing 1.2 mM hydroquinone and 0.50 mM H(2)O(2) by phenylhydrazine, inhibiting the catalytic activity of the HRP enzyme in the reduction of H(2)O(2). The system was optimized to realize a reliable determination of phenylhydrazine in the range of 2.5 x 10(-7) to 1.1 x 10(-6) M with a detection limit of 8.2 x 10(-8) M and a correlation coefficient of 0.999. The modified electrode displayed good reproducibility, sensitivity and stability for the determination of phenylhydrazine.
Yang
Zhou-Sheng
ZS
College of Chemistry and Materials Science, Anhui Key Laboratory of Chemo-Biosensing, Anhui Normal University, Wuhu, PR China. yzhoushe@mail.ahnu.edu.cn
Wu
Wei-Li
WL
Chen
Xi
X
Liu
Yun-Chun
YC
D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002021:Buffers; D002384:Catalysis; D004024:Dicyclohexylcarbodiimide / Q000737:chemistry / Q000378:metabolism; D017097:Electric Impedance; D004563:Electrochemistry; D004800:Enzymes, Immobilized; D006735:Horseradish Peroxidase / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D010659:Phenylhydrazines / Q000032:analysis*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012996:Solutions; D013057:Spectrum Analysis / Q000295:instrumentation / Q000379:methods*; D013388:Succinimides / Q000737:chemistry / Q000378:metabolism
D002021:Buffers; D004800:Enzymes, Immobilized; D010659:Phenylhydrazines; D012996:Solutions; D013388:Succinimides; C030299:phenylhydrazine; D004024:Dicyclohexylcarbodiimide; D006861:Hydrogen Peroxide; D006735:Horseradish Peroxidase; C001426:N-hydroxysuccinimide