title: [Heterogeneity of double-stranded DNA local structure and dynamics: ultrasound studies].
abstract: A method for studying the local inhomogeneities of DNA and its dynamics is proposed. The method is based on the combination of two procedures, splitting the DNA molecules by ultrasound and analysis of DNA fragments obtained by gel electrophoresis. The frequency of cleavage of internucleotide bonds was found to depend on the type of nucleotides forming the bond and on their nearest neighbors. Estimates of cleavage frequencies in each of 16 dinucleotides showed that, in the 5'-d(CpG)-3', 5'-d(CpA)-3', and 5'-d(CpT)-3' dimers, the cleavage occurs considerably more frequently than in the rest, and the frequency of cleavage depends on the nearest neighbors. It was shown that the double-helix cleavage can occur with shifts by several nucleotides. Physical prerequisites were considered that can lead to this pattern of sequence - specific cleavage.
authors.forename: S L
authors.initials: SL
authors.lastname: Grokhovskiĭ
authors.forename: I A
authors.initials: IA
authors.lastname: Il'icheva
authors.forename: D Iu
authors.initials: DIu
authors.lastname: Nechipurenko
authors.forename: L A
authors.initials: LA
authors.lastname: Panchenko
authors.forename: R V
authors.initials: RV
authors.lastname: Polozov
authors.forename: Iu D
authors.initials: IuD
authors.lastname: Nechipurenko
mesh_terms: D004247:DNA / Q000737:chemistry*; D004249:DNA Damage; D019281:Dimerization; D009690:Nucleic Acid Conformation; D009841:Oligonucleotides / Q000737:chemistry*; D010957:Plasmids; D014465:Ultrasonics
chemical_list: D009841:Oligonucleotides; D004247:DNA