title: Ligand binding mode of GABAA receptor-associated protein.
abstract: The gamma-aminobutyric acid type A (GABA(A)) receptor-associated protein is a versatile adaptor protein playing an important role in intracellular vesicle trafficking, particularly in neuronal cells. We present the X-ray structure of the soluble form of human GABA(A) receptor-associated protein complexed with a high-affinity synthetic peptide at 1.3 A resolution. The data shed light on the probable binding modes of key interaction partners, including the GABA(A) receptor and the cysteine protease Atg4. The resulting models provide a structural background for further investigation of the unique biological properties of this protein.
authors.affiliation: Institut für Neurowissenschaften und Biophysik, Molekulare Biophysik, Forschungszentrum Jülich, D-52425 Jülich, Germany.
authors.forename: Oliver H
authors.initials: OH
authors.lastname: Weiergräber
authors.forename: Thomas
authors.initials: T
authors.lastname: Stangler
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Thielmann
authors.forename: Jeannine
authors.initials: J
authors.lastname: Mohrlüder
authors.forename: Katja
authors.initials: K
authors.lastname: Wiesehan
authors.forename: Dieter
authors.initials: D
authors.lastname: Willbold
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000737:chemistry / Q000378:metabolism*; D051017:Apoptosis Regulatory Proteins; D006801:Humans; D007700:Kinetics; D008024:Ligands; D009682:Magnetic Resonance Spectroscopy; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D010455:Peptides / Q000378:metabolism; D011485:Protein Binding; D017433:Protein Structure, Secondary; D020349:Surface Plasmon Resonance
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D051017:Apoptosis Regulatory Proteins; C116741:GABARAP protein, human; D008024:Ligands; D008869:Microtubule-Associated Proteins; D010455:Peptides