Alteration of Autographa californica nuclear polyhedrosis virus DNA upon serial passage in cell culture.
Plaque-purified Autographa californica nuclear polyhedrosis virus, AcMNPV E2 isolate, was propagated for 30 generations by serial passage in TN-368 cells. After passage, the DNAs from 7 of 20 replaque-purified isolates were found to have additional EcoRI restriction enzyme fragments. These 7 isolates were of three types. The additional EcoRI fragments of each isolate hybridized to existing viral DNA. Restriction enzyme mapping with SacI and SmaI show that isolates of each type contain an insertion of repeated viral DNA in approximately the same region of the genome. The origin and location of this DNA is discussed.
Burand
J P
JP
Department of Entomology Texas A&M University, College Station, Texas 77843, USA.
Summers
M D
MD