title: Intrasplenic trafficking of natural killer cells is redirected by chemokines upon inflammation.
abstract: The spleen is a major homing site for NK cells. How they traffic to and within this site in homeostatic or inflammatory conditions is, however, mostly unknown. Here we show that NK cells enter the spleen through the marginal sinus and home to the red pulp via a pertussis toxin-insensitive mechanism. Upon inflammation induced by poly(I:C) injection or mouse cytomegalovirus infection, many NK cells left the red pulp while others transiently entered the white pulp, predominantly the T cell area. This migration was dependent on both CXCR3 and CCL5, suggesting a synergy between CXCR3 and CCR5, and followed the path lined by fibroblastic reticular cells. Thus, the entry of NK cells in the white pulp is limited by the expression of pro-inflammatory chemokines. This phenomenon ensures the segregation of NK cells outside of the white pulp and might contribute to the control of immunopathology.
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Université de la Méditerranée, Marseille, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Grégoire
authors.forename: Céline
authors.initials: C
authors.lastname: Cognet
authors.forename: Lionel
authors.initials: L
authors.lastname: Chasson
authors.forename: Charles-Antoine
authors.initials: CA
authors.lastname: Coupet
authors.forename: Marc
authors.initials: M
authors.lastname: Dalod
authors.forename: Andrea
authors.initials: A
authors.lastname: Reboldi
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Marvel
authors.forename: Federica
authors.initials: F
authors.lastname: Sallusto
authors.forename: Eric
authors.initials: E
authors.lastname: Vivier
authors.forename: Thierry
authors.initials: T
authors.lastname: Walzer
mesh_terms: D000818:Animals; D002465:Cell Movement; D018946:Chemokine CCL5 / Q000502:physiology*; D007249:Inflammation / Q000276:immunology*; D007694:Killer Cells, Natural / Q000502:physiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D037342:Pertussis Toxin / Q000494:pharmacology; D011070:Poly I-C / Q000494:pharmacology; D054367:Receptors, CXCR3 / Q000502:physiology*; D013154:Spleen / Q000276:immunology*
chemical_list: C503460:Ccl5 protein, mouse; D018946:Chemokine CCL5; C515188:Cxcr3 protein, mouse; D054367:Receptors, CXCR3; D037342:Pertussis Toxin; D011070:Poly I-C