Automated measurement of the static light scattering of macromolecular solutions over a broad range of concentrations.
A method and apparatus for automated measurement of the concentration dependence of static light scattering of protein solutions over a broad range of concentrations is described. The gradient of protein concentrations is created by successive dilutions of an initially concentrated solution contained within the scattering measurement cell, which is maintained at constant total volume. The method is validated by measurement of the concentration dependence of light scattering of bovine serum albumin, ovalbumin, and ovomucoid at concentrations up to 130 g/L. The experimentally obtained concentration dependence of scattering obtained from all three proteins is quantitatively consistent with the assumption that no significant self-association occurs over the measured range of concentrations.
Fernández
Cristina
C
Section on Physical Biochemistry, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, US Department of Health and Human Services, Building 8, Bethesda, MD 20892, USA.
Minton
Allen P
AP
D008027:Light; D010047:Ovalbumin / Q000032:analysis; D010059:Ovomucin / Q000032:analysis; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D012542:Scattering, Radiation; D012710:Serum Albumin, Bovine / Q000032:analysis; D012996:Solutions
D011506:Proteins; D012996:Solutions; D012710:Serum Albumin, Bovine; D010059:Ovomucin; D010047:Ovalbumin