Consequence of the loss of Sox2 in the developing brain of the mouse.
The transcription factor Sox2 is expressed at high levels in neural stem and progenitor cells. Here, we inactivated Sox2 specifically in the developing brain by using Cre-loxP system. Although mutant animals did not survive after birth, analysis of late gestation embryos revealed that loss of Sox2 causes enlargement of the lateral ventricles and a decrease in the number of neurosphere-forming cells. However, although their neurogenic potential is attenuated, Sox2-deficient neural stem cells retain their multipotency and self-renewal capacity. We found that expression level of Sox3 is elevated in Sox2 null developing brain, probably mitigating the effects of loss of Sox2.
Miyagi
Satoru
S
Division of Developmental Biology, Research Center for Genomic Medicine, Saitama Medical University, 1397-1 Yamane, Hidaka, Saitama 350-1241, Japan.
Masui
Shinji
S
Niwa
Hitoshi
H
Saito
Tetsuichiro
T
Shimazaki
Takuya
T
Okano
Hideyuki
H
Nishimoto
Masazumi
M
Muramatsu
Masami
M
Iwama
Atsushi
A
Okuda
Akihiko
A
D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D004268:DNA-Binding Proteins / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism; D020868:Gene Silencing; D024002:HMGB Proteins / Q000235:genetics / Q000502:physiology*; D006609:High Mobility Group Proteins / Q000096:biosynthesis; D020547:Lateral Ventricles / Q000166:cytology / Q000196:embryology* / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D051880:Receptors, Notch / Q000378:metabolism; D055748:SOXB1 Transcription Factors; D015398:Signal Transduction / Q000235:genetics; D014157:Transcription Factors / Q000096:biosynthesis / Q000235:genetics / Q000502:physiology*
D004268:DNA-Binding Proteins; D024002:HMGB Proteins; D006609:High Mobility Group Proteins; D051880:Receptors, Notch; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; C527786:Sox3 protein, mouse; D014157:Transcription Factors