title: Hydrogen peroxide induces heme oxygenase-1 and dentin sialophosphoprotein mRNA in human pulp cells.
abstract: Although the induction of heme oxygenase-1 (HO-1) by hydrogen peroxide (H2O2) has been reported, the HO-1 and odontoblastic differentiation-inducing effects against H2O2 have not been clarified in human pulp cells. In this study, we investigated whether HO-1 is involved in the protective mechanisms against the cytotoxic effects of H2O2 by using a cell viability assay, and we examined the production of dentin sialophosphoprotein (DSPP) and other mineralization markers by using reverse transcriptase-polymerase chain reaction in human pulp cells. H2O2 decreased cell viability but increased HO-1 and DSPP expression in a concentration- and time-dependent manner. Antioxidants and inhibitors of HO-1, phosphatidylinositol-3'-kinase, extracellular signal-regulated kinase, and p38 mitogen-activated protein kinase blocked H2O2-induced cytotoxicity and the expression of HO-1 and DSPP mRNA in pulp cells. These data suggest that the induction of HO-1 by H2O2 in pulp cells plays a protective role against the cytotoxic effects of H2O2 and stimulates DSPP expression, resulting in premature odontoblast differentiation through pathways that involve phosphatidylinositol-3'-kinase, p38, and extracellular signal-regulated kinase.
authors.affiliation: Department of Conservative Dentistry, College of Dentistry, Wonkwang University, Iksan, Republic of Korea.
authors.forename: Kyung-San
authors.initials: KS
authors.lastname: Min
authors.forename: Hwa-Jeong
authors.initials: HJ
authors.lastname: Lee
authors.forename: Suk-Ho
authors.initials: SH
authors.lastname: Kim
authors.forename: Sun-Kyung
authors.initials: SK
authors.lastname: Lee
authors.forename: Hyung-Ryong
authors.initials: HR
authors.lastname: Kim
authors.forename: Hyun-Ock
authors.initials: HO
authors.lastname: Pae
authors.forename: Hun-Taeg
authors.initials: HT
authors.lastname: Chung
authors.forename: Hong-In
authors.initials: HI
authors.lastname: Shin
authors.forename: Suk-Keun
authors.initials: SK
authors.lastname: Lee
authors.forename: Eun-Cheol
authors.initials: EC
authors.lastname: Kim
mesh_terms: D015153:Blotting, Western; D002454:Cell Differentiation; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D019610:Cytoprotection; D003782:Dental Pulp / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016326:Extracellular Matrix Proteins / Q000096:biosynthesis*; D051547:Heme Oxygenase-1 / Q000096:biosynthesis / Q000502:physiology*; D006801:Humans; D006861:Hydrogen Peroxide / Q000633:toxicity*; D020935:MAP Kinase Signaling System / Q000187:drug effects; D009804:Odontoblasts / Q000166:cytology; D016877:Oxidants / Q000633:toxicity*; D018384:Oxidative Stress; D010750:Phosphoproteins / Q000096:biosynthesis; D012333:RNA, Messenger / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012795:Sialoglycoproteins; D014073:Tooth Bleaching / Q000009:adverse effects; D000099311:Dentin Sialophosphoprotein
chemical_list: D016326:Extracellular Matrix Proteins; D051547:Heme Oxygenase-1; D006861:Hydrogen Peroxide; D016877:Oxidants; D010750:Phosphoproteins; D012333:RNA, Messenger; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein; C513309:DMP1 protein, human