[Effects of electroacupuncture pretreatment on ischemic arrhythmia and intracellular calcium oscillation in single cardiocyte isolated from rats with myocardial ischemia and reperfusion].
OBJECTIVE
To observe the inhibitory effects of acupuncture pretreatment on arrhythmia and intracellular calcium ([Ca2+]i) oscillation in single cardiocyte in rats subjected to myocardial ischemia-reperfusion (MIR).


METHODS
Male SD rats were evenly randomized into normal control (NC), MIR and electroacupuncture (EA) groups. MIR was performed by ligation (30 min) and reperfusion (10 min) of the left anterior descending coronary artery in vivo in rats or in the isolated and perfused rat heart subjected to a 40 min global ischemia followed by a 10 min reperfusion. EA pretreatment was applied to bilateral "Neiguan" (PC 6) for 30 min once a day in 3 consecutive days. Arrhythmic score was calculated according to Curtis's and Walker's method, and the count of [Ca2+]i oscillation was measured by using InCytPm 2 intracellular ion detecting system.


RESULTS
Both the arrhythmic score and the count of [Ca2+]i oscillation in MIR group were considerably higher than those in NC group (P<0.05). While compared with MIR group, the arrhythmia score and the count of [Ca2+]i oscillation in EA group were decreased significantly (P<0.05).


CONCLUSIONS
Pretreatment of EA can effectively resist MIR-induced arrhythmia and intracellular calcium oscillation in the rat.
Qin
Lian-Ping
LP
Institute of Acu-moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China. qlp1976@yahoo.com.cn
Gao
Jun-Hong
JH
Fu
Wei-Xing
WX
Lu
Bo
B
Cui
Hai-Feng
HF
Wang
Yu-Min
YM
Yu
Xiao-Chun
XC
D000818:Animals; D001145:Arrhythmias, Cardiac / Q000628:therapy*; D020013:Calcium Signaling; D015671:Electroacupuncture; D008297:Male; D017202:Myocardial Ischemia / Q000150:complications*; D015425:Myocardial Reperfusion; D032383:Myocytes, Cardiac / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D011943:Receptors, Adrenergic, beta / Q000502:physiology
D011943:Receptors, Adrenergic, beta