Directed aggregation and fusion of lipid vesicles induced by DNA-surfactants.
We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle.
Maruyama
Tatsuo
T
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, Japan. tmarutcm@crystal.kobe-u.ac.jp
Yamamura
Hiroshi
H
Hiraki
Mai
M
Kemori
Yoshinori
Y
Takata
Harumi
H
Goto
Masahiro
M
D004247:DNA / Q000737:chemistry*; D004262:DNA Restriction Enzymes; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes; D008561:Membrane Fusion; D010713:Phosphatidylcholines / Q000737:chemistry*; D012965:Sodium Chloride / Q000737:chemistry; D013501:Surface-Active Agents / Q000737:chemistry*; D013696:Temperature
D008051:Lipid Bilayers; D008081:Liposomes; D010713:Phosphatidylcholines; D013501:Surface-Active Agents; D012965:Sodium Chloride; D004247:DNA; D004262:DNA Restriction Enzymes; C028694:1-palmitoyl-2-oleoylphosphatidylcholine