Simple and sensitive HPLC method with fluorescence detection for the measurement of ibuprofen in rat plasma: application to a long-lasting dosage form.
A simple and sensitive high-performance liquid chromatography (HPLC) assay applied to the measurement of ibuprofen in rat plasma has been developed. Two parameters have been investigated to improve ibuprofen detectability using fluorescence detection: variation of mobile phase pH and the use of beta-cyclodextrin (beta-CD). Increasing the pH value from 2.5 to 6.5 and adding 5 mM beta-CD enhanced the fluorescence signal (lambda(exc) = 224 nm; lambda(em) = 290 nm) by 2.5 and 1.3-fold, respectively, when using standards. In the case of plasma samples, only pH variation significantly lowered detection and quantification limits, down to 10 and 35 ng/mL, respectively. Full selectivity was obtained with a single step for plasma treatment, that is, protein precipitation with acidified acetonitrile. The validated method was applied to a pharmacokinetic study of ibuprofen encapsulated in microspheres and subcutaneously administered to rats.
Hassan
Ahmed Sheikh
AS
Laboratoire de Pharmacie Galenique et Biopharmacie, Faculte de Pharmacie, Nancy University, Nancy Cedex, France.
Sapin
Anne
A
Ubrich
Nathalie
N
Maincent
Philippe
P
Bolzan
Claire
C
Leroy
Pierre
P
D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D047392:beta-Cyclodextrins / Q000737:chemistry
D047392:beta-Cyclodextrins; C031215:betadex; D007052:Ibuprofen