Analysis of defectiveness of Rous sarcoma virus. IV. Kinetics of RSV production.
The growth kinetics of a defective strain of RSV was studied under two different conditions. In the first, helper viruses having different growth rates were added to non-virus-producing Rous cells in which infection by RSV without helper virus had been established. In the second, RSV was added to chick embryo cells which had previously been infected with helper virus and which were, therefore, in full production of viral coats. In the first instance, the initial rate of RSV production was regulated by the growth rate of the helper virus added; in the second, the initial rate of RSV production was independent of that of the helper virus used for preinfection of cells. The results strongly support the idea that the rate of RSV production depends on the supply of viral coat from the helper virus.
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Virus Laboratory, University of California, Berkeley, California, USA.
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