title: Application of quantitative gene expression analysis for pertussis vaccine safety control.
abstract: Although vaccines are routinely used to prevent infectious diseases, little is known about the comprehensive influences caused by vaccines. In this study, we showed, using comprehensive gene expression analysis, that pertussis vaccine affected many genes in multiple organs of vaccine-treated animals. In particular, lung was revealed to be the most suitable target to evaluate pertussis vaccine toxicity. The 13 genes identified from the analysis of vaccine-treated lung at day 1 showed a clear dendrogram corresponding to pertussis vaccine toxicity. Furthermore, quantitative analysis of these genes revealed a positive correlation between their respective expression levels and the degree of toxic effects observed in samples that had been treated with various doses of reference pertussis vaccines. The quantification of this 13 gene-set is an indicator of the vaccine toxicity-related reaction.
authors.affiliation: Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
authors.forename: Isao
authors.initials: I
authors.lastname: Hamaguchi
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Imai
authors.forename: Haruka
authors.initials: H
authors.lastname: Momose
authors.forename: Mika
authors.initials: M
authors.lastname: Kawamura
authors.forename: Takuo
authors.initials: T
authors.lastname: Mizukami
authors.forename: Seishiro
authors.initials: S
authors.lastname: Naito
authors.forename: Jun-ichi
authors.initials: J
authors.lastname: Maeyama
authors.forename: Atsuko
authors.initials: A
authors.lastname: Masumi
authors.forename: Madoka
authors.initials: M
authors.lastname: Kuramitsu
authors.forename: Kazuya
authors.initials: K
authors.lastname: Takizawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kato
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Horiuchi
authors.forename: Nobuo
authors.initials: N
authors.lastname: Nomura
authors.forename: Shinya
authors.initials: S
authors.lastname: Watanabe
authors.forename: Kazunari
authors.initials: K
authors.lastname: Yamaguchi
mesh_terms: D000818:Animals; D001835:Body Weight; D020869:Gene Expression Profiling / Q000379:methods*; D008168:Lung / Q000187:drug effects; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010567:Pertussis Vaccine / Q000009:adverse effects* / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
chemical_list: D010567:Pertussis Vaccine