Analysis of the in vitro coding properties of the 3' region of turnip yellow mosaic virus genomic RNA.
The genomic RNA of turnip yellow mosaic virus (TYMV) codes in vitro for two proteins of 195,000 (195K) and 150,000 (150K) daltons initiated at the same site of the RNA. In the presence of yeast amber suppressor tRNA, synthesis of the 195K protein is reduced in favor of a 210,000-dalton (210K) protein. Using TYMV RNA sequence data and comparison of tryptic peptides of the 150K, 195K, 210K, and coat proteins, we postulate that the silent region between the 195K gene and the coat protein gene located in the 3' region of TYMV genomic RNA is only 14 nucleotides long.
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Laboratoire de Biochimie du Développement, Institut de Recherche en Biologie Moléculaire du CNRS et de l'Université Paris VII, Tour 43-33, 2 Place Jussieu, 75251, Paris, cedex 05, France.
Drugeon
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Benicourt
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