title: Intestinal intraepithelial NK and NKT cell ontogeny in Lewis rats.
abstract: Natural killer T (NKT) cells have been described in the liver and spleen of adult rats, but their presence and function in other tissues and in early life remains uncertain. This study was designed to determine the proportion of NK cells and NKT cells among small intestine intraepithelial (IE) lymphocytes in suckling rats and adult animals by flow cytometry. Very few intestinal IE-NKT cells (NKR-P1A+ TCRalphabeta+) were present in adult rats ( approximately 1%), but a high proportion of this population was found during early life ( approximately 40% of IE lymphocytes in 9-day-old rats), with a marked age-decreasing pattern. Most of these cells presented the CD8alphabeta+ phenotype. Intestinal IE-NK cells (NKR-P1A+ TCRalphabeta-) were also present in a relatively high proportion during the suckling period ( approximately 30% of IE lymphocytes). Thus, a predominance of both NK and NKT cell subpopulations in small intestine epithelium is characteristic in the early life of rats and may have a protective role during the suckling period.
authors.affiliation: Department of Physiology, Faculty of Pharmacy, University of Barcelona, Av. Joan XXIII s/n, E-08028 Barcelona, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Marín-Gallén
authors.forename: Francisco J
authors.initials: FJ
authors.lastname: Pérez-Cano
authors.forename: Margarida
authors.initials: M
authors.lastname: Castell
authors.forename: Cristina
authors.initials: C
authors.lastname: Castellote
authors.forename: Angels
authors.initials: A
authors.lastname: Franch
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000276:immunology*; D005260:Female; D007413:Intestinal Mucosa / Q000166:cytology* / Q000276:immunology; D007694:Killer Cells, Natural / Q000166:cytology / Q000276:immunology*; D051381:Rats; D011917:Rats, Inbred Lew; D016176:T-Lymphocyte Subsets / Q000166:cytology / Q000276:immunology*