title: Kinetics study on a novel natural inhibitor of alpha-chymotrypsin.
abstract: The mechanism of inhibition of alpha-chymotrypsin by benzoylsalireposide (1), a phenolic glycoside isolated from Symplocos racemosa, was investigated. Lineweaver-Burk and Dixon plots and their secondary replots showed that 1 was a non-competitive inhibitor of this enzyme with the Ki value of 29.60 microM.
authors.affiliation: Dr. Panjwani Center for Molecular Medicine and Drug Research, HEJ Research Institute of Chemistry, International Centre for Chemical Sciences, University of Karachi, Karachi-75270, Pakistan. vuahmad@cyber.net.pk
authors.forename: Viqar Uddin
authors.initials: VU
authors.lastname: Ahmad
authors.forename: Muhammad Arif
authors.initials: MA
authors.lastname: Lodhi
authors.forename: Muhammad Athar
authors.initials: MA
authors.lastname: Abbasi
authors.forename: Muhammad Iqbal
authors.initials: MI
authors.lastname: Choudhary
mesh_terms: D002918:Chymotrypsin / Q000037:antagonists & inhibitors*; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000494:pharmacology; D019684:Magnoliopsida / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D015842:Serine Proteinase Inhibitors / Q000737:chemistry / Q000302:isolation & purification / Q000493:pharmacokinetics* / Q000494:pharmacology
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D015842:Serine Proteinase Inhibitors; C539398:benzoylsalireposide; D002918:Chymotrypsin; C117608:alpha-chymotrypsin