Development of an enzyme-linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y.
A sandwich enzyme-linked immunosorbent assay was developed to detect ciguatoxin (CTX) in fish tissue. The assay utilizes two antibodies, chicken immunoglobulin Y specific to the ABCD domain of CTX and a mouse monoclonal immunoglobulin G-horseradish peroxidase conjugate specific to the JKLM domain of CTX. The sensitivity, working range, cross reactivity, accuracy, precision, and reproducibility were examined.
Empey Campora
Cara
C
Department of Pathology, John A. Burns School of Medicine, Honolulu, Hawaii 96822, USA. empey@hawaii.edu
Hokama
Yoshitsugi
Y
Yabusaki
Kenichi
K
Isobe
Minoru
M
D000818:Animals; D000911:Antibodies, Monoclonal / Q000032:analysis; D001681:Biological Assay / Q000379:methods; D002645:Chickens / Q000276:immunology*; D036841:Ciguatera Poisoning / Q000175:diagnosis*; D002922:Ciguatoxins / Q000032:analysis* / Q000276:immunology; D003429:Cross Reactions / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D007136:Immunoglobulins / Q000276:immunology*; D051379:Mice; D009447:Neuroblastoma; D011042:Poisons / Q000032:analysis*; D011237:Predictive Value of Tests; D015203:Reproducibility of Results; D017747:Seafood / Q000032:analysis*; D014407:Tumor Cells, Cultured
D000911:Antibodies, Monoclonal; C016512:IgY; D007136:Immunoglobulins; D011042:Poisons; D002922:Ciguatoxins