title: Neutrophil infiltration in normal human skin after exposure to different ultraviolet radiation sources.
abstract: Exposure of the skin to UV radiation can lead to a local infiltration of neutrophils. Not much is known on whether the infiltration of neutrophils in the irradiated skin is UV source dependent. In this study we compared different UV sources (solar-simulated radiation [SSR], narrowband [NB]-UVB, broadband [BB]-UVB and UVA1) in their potency to induce neutrophil infiltration in normal human skin after exposure to two times the minimal erythema dose of UV radiation. Biopsies were collected from irradiated buttock skin 6 and 24 h after irradiation and from nonirradiated skin. The presence, distribution and amount of skin-infiltrated neutrophils were determined using immunohistochemical staining. Analysis revealed that SSR was most effective in inducing neutrophil infiltration. NB-UVB gave a neutrophil influx pattern similar to that seen with SSR but in smaller numbers. BB-UVB and UVA1 were far less potent in inducing neutrophil infiltration compared with SSR or NB-UVB. Our findings indicate that neutrophil infiltration in the UV-irradiated skin is UV source dependent. When the spectra emitted by the different UV sources were compared UVB seemed to be more effective than UVA in inducing neutrophil infiltration. Furthermore, our results suggest that longer wavelengths within the UVB range are mostly responsible for the infiltration of neutrophils in the UV-irradiated skin.
authors.affiliation: Department of Dermatology and Allergology, University Medical Center Utrecht, Utrecht, The Netherlands. p.l.lee@umcutrecht.nl
authors.forename: Peter L
authors.initials: PL
authors.lastname: Lee
authors.forename: Huib
authors.initials: H
authors.lastname: van Weelden
authors.forename: Piet L B
authors.initials: PL
authors.lastname: Bruijnzeel
mesh_terms: D005260:Female; D006262:Health; D006801:Humans; D008297:Male; D020556:Neutrophil Infiltration / Q000276:immunology*; D012867:Skin / Q000276:immunology* / Q000528:radiation effects*; D014466:Ultraviolet Rays; D055815:Young Adult