title: Recovery of Enterococcus faecalis in root canal lumen of patients with primary and secondary endodontic lesions.
abstract: The presence of Enterococcus faecalis in root canal teeth affected by primary and secondary periapical lesions was studied using polymerase chain reaction (PCR) assays. The association between presence of E. faecalis with clinical signs of apical lesions was assessed to evaluate a possible relationship between clinical findings. Microbial samples were obtained from healthy patients affected by different periapical lesions, 79 teeth with primary periapical lesion and 23 with secondary periapical lesion. For each tooth, clinical symptoms and X-ray appearance were examined. E. faecalis was detected in 6 of 79 samples with primary lesion (7.6%), and in 9 of 23 with secondary lesion (39.1%). Suggested association was found between E. faecalis and secondary apical lesions. As regard specific signs and symptoms E. faecalis was more associated with asymptomatic lesions (all p<0.05) than with symptomatic apical lesions. The study confirms the high presence of E. faecalis in secondary apical lesions. However, its effective role in endodontic pathogenesis such as bone periapical lesions needs to be clarified.
authors.affiliation: Department of Dental Science, Master Clinical Endodontology, University of Bologna, Alma Mater Studiorum, Bologna, Italy. chiara.pirani4@unibo.it
authors.forename: Chiara
authors.initials: C
authors.lastname: Pirani
authors.forename: Angelica
authors.initials: A
authors.lastname: Bertacci
authors.forename: Francesca
authors.initials: F
authors.lastname: Cavrini
authors.forename: Federico
authors.initials: F
authors.lastname: Foschi
authors.forename: Giovanni Luca
authors.initials: GL
authors.lastname: Acquaviva
authors.forename: Carlo
authors.initials: C
authors.lastname: Prati
authors.forename: Vittorio
authors.initials: V
authors.lastname: Sambri
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D004269:DNA, Bacterial / Q000235:genetics; D003786:Dental Pulp Cavity / Q000382:microbiology*; D013293:Enterococcus faecalis / Q000302:isolation & purification*; D016908:Gram-Positive Bacterial Infections / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D011671:Pulpitis / Q000382:microbiology* / Q000473:pathology / Q000503:physiopathology; D011859:Radiography; D014070:Tooth / Q000000981:diagnostic imaging
chemical_list: D004269:DNA, Bacterial