Protein biochip array of adhesion molecule expression in peripheral blood of patients with nasal polyposis.
Nasal polyposis is a chronic non-infectious inflammatory disease of the nasal and paranasal cavity mucosa of unknown multifactorial origin in which inflammatory cells, and in particular eosinophils, seem to play a pivotal role. Eosinophil migration from the bloodstream to nasal polyps is considered to be specific and is a complex process involving several different molecules such as ICAM-1, VCAM-1, and L-, P- and E-selectins. The aim of this study was to investigate, using a protein biochip array technology, the concentrations of these molecules in the peripheral blood of a group of patients affected by nasal polyposis. Patients exhibited a significantly higher expression of VCAM-1, E-selectin, and L-selectin compared to healthy controls, and Spearman's rank correlation test limited to the molecules with significant betweengroup differences demonstrated a significant correlation between VCAM-1 and E-selectin, VCAM-1 and L-selectin, and Eselectin and L-selectin. The results of this investigation are in line with those coming from various imunohistochemical analyses, and seem to confirm the role of inflammation in the pathogenesis of nasal polyposis. These molecules may also represent novel therapeutic targets in the treatment of nasal polyps, and may allow the selection of pharmacological prophylactics that would allow effective inhibition of the inflammation induced by a given allergen.
Corsi
M M
MM
Institute of General Pathology, Medical Faculty, University of Milan - Italy. mmcorsi@unimi.it
Pagani
D
D
Dogliotti
G
G
Perona
F
F
Sambataro
G
G
Pignataro
L
L
D000328:Adult; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood* / Q000502:physiology; D008297:Male; D008875:Middle Aged; D009298:Nasal Polyps / Q000097:blood* / Q000209:etiology; D040081:Protein Array Analysis / Q000379:methods*; D019087:Selectins / Q000097:blood* / Q000502:physiology; D019010:Vascular Cell Adhesion Molecule-1 / Q000097:blood* / Q000502:physiology
D019087:Selectins; D019010:Vascular Cell Adhesion Molecule-1; D018799:Intercellular Adhesion Molecule-1