Catalytic durability of magnetoproteoliposomes captured by high-gradient magnetic forces in a miniature fixed-bed reactor.
Cytochrome c-oxidase, used as a model membrane-bound enzyme, was embedded in phospholipid bilayer membranes, attached to nanometer-sized Fe(3)O(4) colloids (so-called magnetoliposomes). In comparison with the lipid-depleted free enzyme, both the activity and the enzymatic stability of the complexes, stored at 4 degrees C, were considerably enhanced. These beneficial properties of magnetoproteoliposomes have been successfully exploited in a magnetically controlled fixed-bed bioreactor, operating in a continuous flow regime. (c) 1996 John Wiley & Sons, Inc.
De Cuyper
M
M
Interdisciplinary Research Centre, Katholieke Universiteit Leuven, Campus Kortrijk, B-8500 Kortrijk, Belgium.
De Meulenaer
B
B
Van der Meeren
P
P
Vanderdeelen
J
J