title: Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Poland: further evidence for the changing epidemiology of MRSA.
abstract: This study reports the isolation of CA-MRSA strain which was found to colonize the nasal mucosa of a patient undergoing haemodialysis treatment. The MRSA was subjected to molecular analysis by Pulsed Field Gel Electrophoresis (PFGE), multiplex PCR assay for staphylococcal cassette chromosome mec (SCCmec) typing, and PCR detection of the pvl gene encoding for Panton-Valentine leukocidin. The analyzed MRSA harbored the SCCmec type IV and the pvl gene-two unique genetic markers of CA-MRSA. The PFGE pattern of the strain corresponded to the common European CA-MRSA (MLST Type ST80). Moreover, the strain was only resistant to beta-lactam agents and tetracycline. This study adds further evidence for the changing epidemiology of MRSA and indicates the ability of CA-MRSA to affect persons with established risk factors in addition to previously healthy individuals.
authors.affiliation: Department of Medical Microbiology, Medical University of Lublin, Lublin, Poland. agola7@wp.pl
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Bogut
authors.forename: Maria
authors.initials: M
authors.lastname: Kozioł-Montewka
authors.forename: Iwona
authors.initials: I
authors.lastname: Baranowicz
authors.forename: Lucyna
authors.initials: L
authors.lastname: Józwiak
authors.forename: Zainab
authors.initials: Z
authors.lastname: Al-Doori
authors.forename: Donald
authors.initials: D
authors.lastname: Morrison
authors.forename: Danuta
authors.initials: D
authors.lastname: Kaczor
authors.forename: Andrzej
authors.initials: A
authors.lastname: Ksiazek
mesh_terms: D015373:Bacterial Typing Techniques; D017714:Community-Acquired Infections / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D016521:Electrophoresis, Gel, Pulsed-Field; D005798:Genes, Bacterial; D005838:Genotype; D006801:Humans; D016106:Methicillin Resistance; D017720:Molecular Epidemiology; D009297:Nasal Mucosa / Q000382:microbiology; D011044:Poland / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004269:DNA, Bacterial