Synthesis and evaluation of the luciferase-oligodeoxynucleotide for the sequence-selective detection of nucleic acids.
A new method for the synthesis of ODN-luciferase conjugate was investigated as a signal-amplifying sensor of the target nucleic acids. The conjugation of the luciferase was successfully achieved between the cysteine residue and the 2-amino-6-vinylpurine nucleoside of the ODN probe without significant inactivation of luciferase. The ODN-luciferase conjugate modified with PEG retained the luciferase activity and selectivity during the hybridization with the target ODN.
Nagatsugi
Fumi
F
Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Japan.
Nakahara
Ryusuke
R
Inoue
Kaori
K
Sasaki
Shigeki
S
D002846:Chromatography, Affinity; D008156:Luciferases / Q000138:chemical synthesis* / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000138:chemical synthesis* / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D017422:Sequence Analysis, DNA / Q000379:methods*
D009841:Oligonucleotides; D011092:Polyethylene Glycols; D008156:Luciferases