Diagnostic utility of interferon-gamma-induced protein of 10 kDa (IP-10) in tuberculous pleurisy.
Tuberculous pleuritis (TP) is characterized by predominant Th1 immune response. We observed significantly high levels of interferon gamma (IFN-gamma) and chemokines such as IP-10, monokine induced by IFN-gamma (MIG), interleukin 8 (IL-8), monocyte chemotactic protein (MCP)-1, and macrophage inflammatory protein (MIP)-1alpha in tuberculous pleural effusions. In the current study, we evaluated the diagnostic utility of IFN-gamma-dependent chemokine especially IP-10. The receiver operating characteristics (ROC) curve analyses based on cytometric bead array values depicted high sensitivity only for IP-10 (76.3%) followed by IFN-gamma (73.7%). The ELISA test further confirmed the significantly high levels of IFN-gamma and IP-10 in TP. The ROC curve analysis again demonstrated high area under the curve (AUC) for IP-10 (0.966) than the referred diagnostic marker IFN-gamma (0.930). The better sensitivity (84.2% for IFN-gamma and 89.2% for IP-10) and equal specificity (95.7%) of IP-10 assay compared with IFN-gamma suggest that IP-10 is a potential diagnostic marker for evaluating TP.
Supriya
Pokkali
P
Department of Immunology, Tuberculosis Research Centre (ICMR), Chennai 600 031, India.
Chandrasekaran
Prabha
P
Das
Sulochana D
SD
D000328:Adult; D000368:Aged; D054357:Chemokine CXCL10 / Q000378:metabolism; D005260:Female; D006801:Humans; D007371:Interferon-gamma / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010996:Pleural Effusion / Q000276:immunology / Q000382:microbiology; D012372:ROC Curve; D012680:Sensitivity and Specificity; D014396:Tuberculosis, Pleural / Q000175:diagnosis* / Q000276:immunology / Q000382:microbiology
C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D007371:Interferon-gamma