title: Simple and sensitive HPLC method with fluorescence detection for the measurement of ibuprofen in rat plasma: application to a long-lasting dosage form.
abstract: A simple and sensitive high-performance liquid chromatography (HPLC) assay applied to the measurement of ibuprofen in rat plasma has been developed. Two parameters have been investigated to improve ibuprofen detectability using fluorescence detection: variation of mobile phase pH and the use of beta-cyclodextrin (beta-CD). Increasing the pH value from 2.5 to 6.5 and adding 5 mM beta-CD enhanced the fluorescence signal (lambda(exc) = 224 nm; lambda(em) = 290 nm) by 2.5 and 1.3-fold, respectively, when using standards. In the case of plasma samples, only pH variation significantly lowered detection and quantification limits, down to 10 and 35 ng/mL, respectively. Full selectivity was obtained with a single step for plasma treatment, that is, protein precipitation with acidified acetonitrile. The validated method was applied to a pharmacokinetic study of ibuprofen encapsulated in microspheres and subcutaneously administered to rats.
authors.affiliation: Laboratoire de Pharmacie Galenique et Biopharmacie, Faculte de Pharmacie, Nancy University, Nancy Cedex, France.
authors.forename: Ahmed Sheikh
authors.initials: AS
authors.lastname: Hassan
authors.forename: Anne
authors.initials: A
authors.lastname: Sapin
authors.forename: Nathalie
authors.initials: N
authors.lastname: Ubrich
authors.forename: Philippe
authors.initials: P
authors.lastname: Maincent
authors.forename: Claire
authors.initials: C
authors.lastname: Bolzan
authors.forename: Pierre
authors.initials: P
authors.lastname: Leroy
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005453:Fluorescence; D006863:Hydrogen-Ion Concentration; D007052:Ibuprofen / Q000008:administration & dosage / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012680:Sensitivity and Specificity; D047392:beta-Cyclodextrins / Q000737:chemistry
chemical_list: D047392:beta-Cyclodextrins; C031215:betadex; D007052:Ibuprofen