[Preparation of hen egg yolk immunoglobulin and partial plasma high abundant protein depletion by prepared chicken immunoglobulin coupled on GoldMag carrier].
OBJECTIVE
To prepare specific egg yolk immunoglobulin (IgY) against HSA and IgG and to make HSA and IgG depletion research in human plasma by binding it to the surface of GoldMag.


METHODS
Laying Roman hens immunized with different antigens, such as HSA, IgG and the mixtures of these proteins were used in the experiments. The best condition of isolating the IgY antibody was studied. The titer and purity of the antibody were examined by indirect ELISA and SDS-PAGE respectively. Then the specific IgY was bound to the surface of GoldMag for HSA and IgG depletion research.


RESULTS
60-120 days after immunization, the titer of the antibody in the plasma against different antigens reached 1:15 000-1:25 000; while the titer in the egg reached 1:10 000-1:25 000. The purity was over 98%. Most of HSA and IgG were depleted from human plasma by IgY connected to GoldMag.


CONCLUSIONS
HSA and IgG can be used as antigens to immunize laying hens to produce IgY with high titer and purity. IgY which is bound to the GoldMag carrier can be used to deplete of the relevant HSA and IgG from human plasma effectively. As a new method of studying proteome of plasma, it has some practical applications.
Lin
Fang
F
Life Science College, Northwest University, Xi'an 710069, China.
Huang
Jian-Xin
JX
Chen
Chao
C
Liu
Jia
J
Cui
Ya-Li
YL
D000818:Animals; D002645:Chickens; D004530:Egg Yolk / Q000378:metabolism*; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology*; D007136:Immunoglobulins / Q000276:immunology*; D012709:Serum Albumin / Q000276:immunology*
C016512:IgY; D007074:Immunoglobulin G; D007136:Immunoglobulins; D012709:Serum Albumin