title: Different effect of   Toxoplasma gondii   infection on adhesion capacity   of fibroblasts and monocytes.
abstract: This study investigated the effect of infection with the apicomplexan parasite Toxoplasma gondii, in combination with the concomitant cytokine environment (IFN-gamma/TNF-alpha), on adhesion of THP-1 monocytic cells to MRC-5 fibroblasts. Surprisingly, infection of THP-1 cells decreased their adhesion to the MRC-5 cell monolayer. This decrease was compensated by IFN-gamma/TNF-alpha stimulation. In contrast, infection of MRC-5 cells significantly increased adhesion, which was synergistically augmented by cytokine stimulation. Levels of ICAM-1 (CD54) on MRC-5 cells, as well as LFA-1 (CD11a) on THP-1 cells, were not changed by infection, neither in resting, nor in cytokine stimulated cells. These results show that T. gondii infection alters adhesion properties and reactivity to cytokine stimulation in a cell-specific way.
authors.affiliation: Institut de Parasitologie et de Pathologie Tropicale, Interaction Cellulaire et Moléculaire Hôte-Parasite (E.A. 3950), Faculté de Médecine, Strasbourg, France. alexander.pfaff@medecine.u-strasbg.fr
authors.forename: A W
authors.initials: AW
authors.lastname: Pfaff
authors.forename: S
authors.initials: S
authors.lastname: Georges
authors.forename: E
authors.initials: E
authors.lastname: Candolfi
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002460:Cell Line; D016207:Cytokines / Q000276:immunology; D005347:Fibroblasts / Q000276:immunology*; D006801:Humans; D009000:Monocytes / Q000166:cytology / Q000276:immunology*; D014122:Toxoplasma / Q000166:cytology / Q000276:immunology*; D014123:Toxoplasmosis / Q000276:immunology*
chemical_list: D016207:Cytokines