title: Surface modification of polymer microfluidic devices using in-channel atom transfer radical polymerization.
abstract: In-channel atom transfer radical polymerization (ATRP) was used to graft a PEG layer on the surface of microchannels formed in poly(glycidyl methacrylate)-co-(methyl methacrylate) (PGMAMMA) microfluidic devices. The patterned and cover plates were first anchored with ATRP initiator and then thermally bonded together, followed by pumping a solution containing monomer, catalyst, and ligand into the channel to perform ATRP. A PEG-functionalized layer was grafted on the microchannel wall, which resists protein adsorption. X-ray photoelectron spectroscopy (XPS) was used to investigate the initiator-bound surface, and EOF was measured to evaluate the PEG-grafted PGMAMMA microchannel. Fast, efficient, and reproducible separations of amino acids, peptides, and proteins were obtained using the resultant microdevices. Separation efficiencies were higher than 1.0x10(4) plates for a 3.5 cm separation microchannel. Compared with microdevices modified using a previously reported ATRP technique, these in-channel modified microdevices demonstrated better long-term stability.
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Sun
authors.forename: Jikun
authors.initials: J
authors.lastname: Liu
authors.forename: Milton L
authors.initials: ML
authors.lastname: Lee
mesh_terms: D000596:Amino Acids / Q000302:isolation & purification; D002364:Caseins / Q000378:metabolism; D019075:Electrophoresis, Capillary / Q000379:methods; D006801:Humans; D007782:Lactoglobulins / Q000302:isolation & purification; D008768:Methylmethacrylates / Q000737:chemistry; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D010455:Peptides / Q000302:isolation & purification; D019904:Polymethyl Methacrylate / Q000737:chemistry; D011492:Protein Hydrolysates / Q000302:isolation & purification; D011506:Proteins / Q000302:isolation & purification; D012709:Serum Albumin / Q000302:isolation & purification
chemical_list: D000596:Amino Acids; D002364:Caseins; D007782:Lactoglobulins; D008768:Methylmethacrylates; D010455:Peptides; D011492:Protein Hydrolysates; D011506:Proteins; D012709:Serum Albumin; C531903:poly(glycidyl methacrylate)-co-(methyl methacrylate); D019904:Polymethyl Methacrylate