title: Vaccination with Bacille-Calmette Guérin promotes mycobacterial control in guinea pig macrophages infected in vivo.
abstract: Tuberculosis pleurisy was induced by inoculation of virulent (H37Rv strain or Erdman strain) or attenuated (H37Ra strain) green-fluorescent protein-expressing Mycobacterium tuberculosis into guinea pigs that had or had not been vaccinated with Bacille-Calmette Guérin (BCG). Pleural fluid and cells were analyzed for phagosome-lysosome (P-L) fusion, on the basis of confocal microscopy, intracellular and extracellular bacterial survival, and production of cytokine mRNA. BCG vaccination increased fluid volume and cellular accumulation, significantly enhanced P-L fusion, and significantly decreased intracellular bacterial survival in pleural-effusion macrophages of the guinea pigs infected with the 2 virulent strains. Furthermore, significant increases in interferon-gamma, transforming growth factor-beta, tumor necrosis factor-alpha, and interleukin-12p40 cytokine mRNA were seen in the pleural cells of the BCG-vaccinated guinea pigs.
authors.affiliation: Department of Microbial and Molecular Pathogenesis, Texas A&M University System Health Science Center, College Station, Texas 77843, USA. lhly@medicine.tamhsc.edu
authors.forename: Lan H
authors.initials: LH
authors.lastname: Ly
authors.forename: Rola
authors.initials: R
authors.lastname: Barhoumi
authors.forename: Sang H
authors.initials: SH
authors.lastname: Cho
authors.forename: Scott G
authors.initials: SG
authors.lastname: Franzblau
authors.forename: David N
authors.initials: DN
authors.lastname: McMurray
mesh_terms: D000818:Animals; D001500:BCG Vaccine / Q000276:immunology*; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006168:Guinea Pigs; D008264:Macrophages / Q000276:immunology* / Q000382:microbiology*; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013047:Specific Pathogen-Free Organisms; D014396:Tuberculosis, Pleural / Q000276:immunology*; D014774:Virulence
chemical_list: D001500:BCG Vaccine; D016207:Cytokines; D012333:RNA, Messenger; D049452:Green Fluorescent Proteins