Messenger RNA for the coat protein of southern bean mosaic virus.
When RNA from southern bean mosaic virus is fractionated on a sucrose gradient, the resulting absorbance profile shows a major peak corresponding to 1.4 x 10(6) MW together with a considerable amount of slower sedimenting material. The RNA from these gradient fractions was translated in wheat embryo extracts and reticulocyte lysates. Only RNA in the molecular weight range 0.3 - 0.4 x 10(6) was found to induce synthesis of coat protein (designated P3). RNA of molecular weight 1.4 x 10(6) induced synthesis of three proteins, P1, P2, and P4.
Rutgers
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Biophysics Laboratory of the Graduate School, and Biochemistry Department of the College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706, USA.
Salerno-Rife
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Kaesberg
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