Development of rat antigen-presenting cells from pluripotent ecto-mesenchymal stem cells in vitro and in vivo.
Dendritic cells (DC) are specialized cells that capture and present antigen to T cells. Recent advances have been made in understanding their origin, heterogeneity, and the signals that induce their migration and maturation resident microglia are antigen-presenting cells (APC) involved in stimulation or reactivation of CNS-targeted T cells. Generation of DC from microglia, as demonstrated ex vivo, may support GM-CSF-driven differentiation of brain DC from local, likely, microglial progenitors. Here, we report the establishment of long-term cultures of rat ecto-mesenchymal stem cells (EMSCs) using specific supplemented media for induction. These EMSCs share some morphological characteristics and the allostimulatory capacity of classical DCs, and when transplanted into the brain using a rat glioma model survive within the cortex, and are morphologically and phenotypically similar to microglia over 7 days. Our findings related to the development and differentiation of microglial progenitors support the view that microglia are derived prenatally from mesodermal progenitors that are distinct from monocytes.
Hu
Shijie
S
Institute of Neurosurgery, Xijing Hospital, The Fourth Military Medical University, Shaanxi, China.
Shen
Xuefeng
X
Zhang
Rong
R
Zhang
Yongjie
Y
Zhang
Rui
R
Zhang
Wei
W
Deng
Zhihong
Z
Cao
Yunxin
Y
Zhou
Zeyuan
Z
Chen
Jinwu
J
Ge
Guanqun
G
Xuan
Kun
K
Zhang
Xiang
X
Jin
Yan
Y
D000818:Animals; D000938:Antigen-Presenting Cells / Q000502:physiology*; D001854:Bone Marrow Cells / Q000166:cytology; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000502:physiology*; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D003713:Dendritic Cells / Q000276:immunology* / Q000648:ultrastructure; D004622:Embryo, Mammalian / Q000166:cytology; D005260:Female; D005910:Glioma / Q000473:pathology; D007150:Immunohistochemistry; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D009368:Neoplasm Transplantation; D039904:Pluripotent Stem Cells / Q000166:cytology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D014184:Transplantation, Homologous
D003470:Culture Media