title: Autonomous DNA computing machine based on photochemical gate transition.
abstract: We report the construction of a one-pot autonomous DNA computing machine based on photochemical gate transition (photocleavage, hybridization, and photoligation), and we performed binary digit additions using this machine. In our method, both photochemical DNA manipulations previously reported, photoligation via 5-carboxyvinyldeoxyuridene (cvU) containing ODN and photocleavage via carbazole-modified ODN, were employed. The binary digit additions were autonomously carried out by one-time irradiation at 366 nm in the single test tube. The fluorescence readout by the DNA chip was in good agreement with the correct answer of binary digit additions. We believe that this system is easily applicable to correlation analysis between SNPs as well as other binary digit processing, such as subtraction.
authors.affiliation: School of Materials Science, Japan Advanced Institute of Science and Technology, Asahidai 1-1, Nomi, Ishikawa, Japan.
authors.forename: Shinzi
authors.initials: S
authors.lastname: Ogasawara
authors.forename: Takehiro
authors.initials: T
authors.lastname: Ami
authors.forename: Kenzo
authors.initials: K
authors.lastname: Fujimoto
mesh_terms: D039301:Computers, Molecular; D004247:DNA; D008432:Mathematical Computing; D010777:Photochemistry
chemical_list: D004247:DNA