[Study of liquorice processing fructus].
OBJECTIVE
To establish the processing method of fructus evodiae and its standard for quality control, toxicity aspects and pharmacodynamics were carried out at the same time.


METHODS
In the studies of processing techniques, the optimized technical parameters were determined by the contents of evodiamine and evodine. And the acute toxicity and pharmacodynamics were studied by rats.


RESULTS
The process was that the liquorice-processed fructus evodiae was wetted by liquorice decoction by sixth of raw fructus evodiae (V/W) and fried below 230 degrees C. The method of detecting the contents of evodiamine and evodine was that Alltima ODS C18 (4.6 mm x 250 mm, 5 microm); mobile phase acetonitrile-water-tetrahydrofuran-phosphoric acid (51:48: 1: 0.05); column temperature 25 degrees C; mobile rate 0.8 mL x min(-1); wave length 225 nm. The toxicity experimentation show that rats didnt show any notable changes after affused the raw material and the processed fructus evodiae's decotion 40 g x kg(-1) b. w. at one time seven days constantly. The analgesic effect was observed after 0.6 g (material) x kg(-1) (weight) b. w.


CONCLUSIONS
The toxicity of the raw material and the processed one were low and the liquorice-processed fructus evodia analgesic effect was good.
Hong
Yu-Mei
YM
Institute of Chinese Materia Medica China, Academy of Chinese Medical Science, Beijing 100700, China.
Wang
Wei-Hao
WH
Wang
Zhi-Min
ZM
Wang
Jin-Hua
JH
D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use / Q000633:toxicity; D000818:Animals; D002845:Chromatography; D004365:Drugs, Chinese Herbal / Q000302:isolation & purification / Q000494:pharmacology / Q000627:therapeutic use / Q000633:toxicity; D005260:Female; D006035:Glycyrrhiza / Q000737:chemistry*; D016014:Linear Models; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D010146:Pain / Q000188:drug therapy; D015203:Reproducibility of Results; D027821:Rutaceae / Q000737:chemistry*; D013696:Temperature
D000700:Analgesics; D004365:Drugs, Chinese Herbal