The effect of dalteparin, a kind of low molecular weight heparin, on lung adenocarcinoma A549 cell line in vitro.
The beneficial effects of unfractionated heparin and low molecular weight heparin on cancer progression have been reported. In the study, the effect of dalteparin on pulmonary adenocarcinoma A549 cell line was observed in vitro. Our experiments revealed that: (1) dalteparin can inhibit the cell viability dose-dependently and time-dependently; (2) dalteparin can arrest the A549 cells in G(1) phase and induce them to early apoptosis; (3) dalteparin exerts its effects through p21(WAF1) and p27(KIP1) proteins; and (4) the changes of p21(WAF1) and p27(KIP1) proteins caused by dalteparin occurred at posttranscriptional levels.
Chen
Xiaojun
X
Department of Respiratory Diseases, Qilu Hospital, Shandong University, Jinan, Shandong, PR China.
Xiao
Wei
W
Qu
Xun
X
Zhou
Shengyu
S
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000925:Anticoagulants / Q000494:pharmacology*; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D050759:Cyclin-Dependent Kinase Inhibitor p21 / Q000235:genetics / Q000378:metabolism; D050760:Cyclin-Dependent Kinase Inhibitor p27; D017985:Dalteparin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D011499:Protein Processing, Post-Translational / Q000187:drug effects; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors
D000925:Anticoagulants; D000970:Antineoplastic Agents; C493956:CDKN1A protein, human; C436291:CDKN1B protein, human; D050759:Cyclin-Dependent Kinase Inhibitor p21; D047908:Intracellular Signaling Peptides and Proteins; D012333:RNA, Messenger; D050760:Cyclin-Dependent Kinase Inhibitor p27; D017985:Dalteparin