title: Factor VII -323 decanucleotide D/I polymorphism in atrial fibrillation: implications for the prothrombotic state and stroke risk.
abstract: There are limited data on the influence of genetic polymorphisms in atrial fibrillation (AF) stroke risk. We hypothesized that a functional haemostatic polymorphism, that is, the factor VII -323 Del/Ins polymorphism, would influence the prothrombotic state associated with AF, as well as stroke risk. Other functional polymorphisms were also tested.
  
  
  METHODS
  We performed a cross-sectional study of 119 AF patients, who were compared to 96 patients with stroke secondary to AF. In the first patient group, we analysed plasma prothrombin fragment 1+2 levels (F1+2, an index of thrombin generation) to reflect the prothrombotic state of AF.
  
  
  RESULTS
  AF patients carrying the -323 Ins allele had lower plasma F1+2 levels (P=0.015). After multivariate analysis adjusted by age, sex and clinical risk factors, advanced age and 807C/T polymorphism of glycoprotein Ia (GPIa) gene were associated with higher risk of ischaemic stroke (OR: 1.06; P=0.003 and OR: 1.91; P=0.025), whilst FVII Ins -323 allele was associated with lower stroke risk (OR: 0.41; P=0.017).
  
  
  CONCLUSIONS
  FVII -323 Ins allele may modulate the prothrombotic state associated with AF. Despite the small sample size, we found that FVII Ins -323 allele could be associated with a lower stroke risk in AF, whereas the 807C/T polymorphism may increase the risk.
authors.affiliation: Centro de Hemodonacíon, Universidad de Murcia, Murcia, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Roldán
authors.forename: Francisco
authors.initials: F
authors.lastname: Marín
authors.forename: Rocío
authors.initials: R
authors.lastname: González-Conejero
authors.forename: Antonio
authors.initials: A
authors.lastname: García-Honrubia
authors.forename: Silvia
authors.initials: S
authors.lastname: Martí
authors.forename: Aranzazu
authors.initials: A
authors.lastname: Alfaro
authors.forename: Mariano
authors.initials: M
authors.lastname: Valdés
authors.forename: Javier
authors.initials: J
authors.lastname: Corral
authors.forename: Gregory Y H
authors.initials: GY
authors.lastname: Lip
authors.forename: Vicente
authors.initials: V
authors.lastname: Vicente
mesh_terms: D001281:Atrial Fibrillation / Q000097:blood / Q000150:complications* / Q000235:genetics*; D016022:Case-Control Studies; D003430:Cross-Sectional Studies; D005167:Factor VII / Q000235:genetics*; D005260:Female; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics*; D008297:Male; D010446:Peptide Fragments / Q000097:blood; D011110:Polymorphism, Genetic / Q000235:genetics*; D011498:Protein Precursors / Q000097:blood; D011516:Prothrombin; D020521:Stroke / Q000235:genetics*
chemical_list: D039421:Integrin alpha2; D010446:Peptide Fragments; D011498:Protein Precursors; C029733:prothrombin fragment 1; C044183:prothrombin fragment 2; D005167:Factor VII; D011516:Prothrombin