Calculation of the isoelectric point of tryptic peptides in the pH 3.5-4.5 range based on adjacent amino acid effects.
Current algorithms for the calculation of peptide or protein pI, based on the charge associated with individual amino acids, can calculate pI values to within +/-0.2 pI units. Here, we present a new pI calculation algorithm that takes into account the effect of adjacent amino acids on the pI value. The algorithm accounts for the effect of adjacent amino acids+/-3 residues away from a charged aspartic or glutamic acid, as well as effects on the free C terminus, and applies a correction term to the corresponding pK values. The correction increments are derived from a 5000-peptide training set using a genetic optimization approach. The accuracy of the new pI values obtained with this method approaches the error associated with the manufacture of the IPG strip (<+/-0.03 pI units). The approach is demonstrated for cytosolic cell extracts derived from the breast-cancer cell line DU4475, and from membrane preparations from human lung-tissue samples. One potential application of a more highly accurate pI calculation is data filtering of MS/MS outputs that will allow for more complex database searches including gene finding, and validation, and detection of coding single-nucleotide polymorphisms in their expressed form.
Cargile
Benjamin J
BJ
Mass Spectrometry Research Program, Research Triangle Institute, Research Triangle Park, NC, USA.
Sevinsky
Joel R
JR
Essader
Amal S
AS
Eu
Jerry P
JP
Stephenson
James L
JL
D000465:Algorithms; D000596:Amino Acids / Q000737:chemistry*; D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007525:Isoelectric Focusing / Q000379:methods*; D007526:Isoelectric Point; D008297:Male; D011492:Protein Hydrolysates / Q000737:chemistry*; D051381:Rats; D013737:Testis / Q000737:chemistry; D014357:Trypsin / Q000378:metabolism
D000596:Amino Acids; D011492:Protein Hydrolysates; D014357:Trypsin