[Effects of LH analogs on the secretion of NGF in submaxillary gland cells of rats in vitro].
OBJECTIVE
To investigate the effects of luteinizing hormone (LH) analogs on the secretion of nerve growth factor (NGF) in submandibular gland cells of rats in vitro.


METHODS
The submandibular gland cells of rats were incubated in vitro and LH analogs at various concentration were added to the medium. The never growth factor (NGF) was detected by enzyme-link immunoassay (ELISA).


RESULTS
When LH analogs were at the concentration of 10(-6), 10(-4), 10(-2) U/L respectively, the secretion of NGF was gradually increased. However, when LH analogs were at the concentration of 10(-2), 10(0), 10(2) U/L respectively, the secretion of NGF was gradually decreased. When LH analogs (10(-5) IU/mL) were incubated at different time, the secretion of NGF was different. NGF reached the peak value when it was incubated for 8 h and then decreased gradually.


CONCLUSIONS
LH analogs may regulate the secretion of NGF in the submaxillary gland cells of rats.
Chen
Lei
L
Department of Gynaecology and Obstetrics, Naval General Hospital, Beijing 100037, China. chenleis@mail.tsinghua.edu.cn
Bai
Hong-Wei
HW
Sun
Xu-de
XD
Zhao
Jun
J
D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D007986:Luteinizing Hormone / Q000031:analogs & derivatives* / Q000494:pharmacology*; D008297:Male; D020932:Nerve Growth Factor / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013363:Submandibular Gland / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*; D013997:Time Factors
D007986:Luteinizing Hormone; D020932:Nerve Growth Factor