title: Allosensitization to HLA and MICA is an important measure of renal graft outcome.
abstract: The objective of this study was to evaluate the donor-specific antibody repertoire against T and B cells and monocytes, as well as the non-donor anti-HLA, and MICA (MHC class I-related chain A) antibodies in recipients of the live related donor renal transplantation. Sera collected before and after transplantation were tested by ELISA for the presence of HLA class I- and class II-specific antibodies and by Luminex MICA single-antigen bead assay for the detection of MICA antibodies. Patients having a combination of both anti-HLA and MICA antibodies had worse graft survival and more rejection episodes as compared to the group without antibodies. Further, presence of IgG antibodies against the donor cells (T, B & monocytes) led to a compromised graft survival along with higher incidence of acute rejection as compared to the negative groups. These results suggest that a comprehensive assessment of anti-donor antibody repertoire and monitoring of anti-HLA, MICA antibodies following transplantation is a useful exercise to detect the sensitization status of the recipient and this can prove to be of immense prognostic value in renal transplantation.
authors.affiliation: Department of Transplant Immunology & Immunogenetics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi, India.
authors.forename: A
authors.initials: A
authors.lastname: Panigrahi
authors.forename: J A
authors.initials: JA
authors.lastname: Siddiqui
authors.forename: A
authors.initials: A
authors.lastname: Rai
authors.forename: A
authors.initials: A
authors.lastname: Margoob
authors.forename: A
authors.initials: A
authors.lastname: Khaira
authors.forename: D
authors.initials: D
authors.lastname: Bhowmik
authors.forename: S C
authors.initials: SC
authors.lastname: Tiwari
authors.forename: S
authors.initials: S
authors.lastname: Guleria
authors.forename: N K
authors.initials: NK
authors.lastname: Mehra
mesh_terms: D000918:Antibody Specificity; D015415:Biomarkers / Q000097:blood; D006084:Graft Rejection / Q000276:immunology*; D006085:Graft Survival / Q000276:immunology*; D006680:HLA Antigens / Q000276:immunology*; D015395:Histocompatibility Antigens Class I / Q000276:immunology*; D006650:Histocompatibility Testing; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood / Q000276:immunology; D007518:Isoantibodies / Q000097:blood / Q000276:immunology; D016030:Kidney Transplantation / Q000276:immunology*; D019520:Living Donors; D012189:Retrospective Studies; D014184:Transplantation, Homologous; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D006680:HLA Antigens; D015395:Histocompatibility Antigens Class I; D007074:Immunoglobulin G; D007518:Isoantibodies; C099187:MHC class I-related chain A