Construction of high-resolution comparative maps in mammals using BAC-end sequences.
Whole-genome bacterial artificial chromosome (BAC) libraries for approximately 40 representative species of mammals provide unique resources for the construction of high-resolution ordered genomic maps, the development of species genetics, and multispecies genome comparisons. Herein we describe procedures to construct high-quality radiation hybrid comparative maps using BAC-end sequences (BESs). This approach has been applied to the construction of approximately 1 Mbp resolution porcine-human and cattle-human comparative maps. High-resolution ordered comparative maps built with BESs and whole-genome sequences have been shown to be an invaluable resource for the analysis of mammalian chromosome evolution, and as a backbone for the assembly of whole-genome shotgun sequences.
Larkin
Denis M
DM
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Lewin
Harris A
HA
D000818:Animals; D001483:Base Sequence; D002417:Cattle; D002874:Chromosome Mapping / Q000379:methods*; D022202:Chromosomes, Artificial, Bacterial / Q000235:genetics*; D002999:Clone Cells; D004247:DNA / Q000302:isolation & purification; D017931:DNA Primers; D016678:Genome / Q000235:genetics; D005838:Genotype; D006801:Humans; D008322:Mammals / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011786:Quality Control; D021101:Radiation Hybrid Mapping; D012015:Reference Standards; D017422:Sequence Analysis, DNA; D013552:Swine
D017931:DNA Primers; D004247:DNA