Detection of the antibody to lymphocytic choriomeningitis virus in sera of laboratory rodents infected with viruses of laboratory and newly isolated strains by ELISA using purified recombinant nucleoprotein.
An enzyme-linked immunosorbent assay (ELISA) was developed to detect the antibody against lymphocytic choriomeningitis virus (LCMV) in sera of laboratory animals. In this ELISA system, LCMV-nucleoprotein (NP) expressed by recombinant baculovirus and purified with high molar urea was used as the antigen. Sera from laboratory animals experimentally infected with the Armstrong strain or the newly isolated M1 strain of LCMV were examined to detect anti-LCMV antibody by the ELISA system, and the reactivity was compared with that of IFA test. Regardless of LCMV strain, all the sera of adult mice infected with LCMV were positive with very high optical density (OD). Also, the sera from mice neonatally infected with LCMV M1 strain were positive with slightly lower OD than adult mice. In contrast, all the sera of uninfected mice were negative to LCMV-NP antigen. Similarly, anti-LCMV antibodies were detected in all the sera of hamsters, mastomyses, and gerbils infected with the LCMV Armstrong strain. The results of the ELISA were in complete agreement with those of IFA, and indicate the high sensitivity and specificity of the ELISA system in the detection of anti-LCMV antibody. Because this ELISA system does not require handling infectious LCMV in the course of the antigen preparation and serological assay, there is no risk of contamination in the laboratory or nearby animal facility. In addition, by using negative control antigen in parallel with positive antigen in ELISA, we can exactly check the LCMV contamination in laboratory animals.
Takimoto
Kazuhiro
K
Division of Experimental Animal Research, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
Taharaguchi
Motoko
M
Morikawa
Shigeru
S
Ike
Fumio
F
Yamada
Yasuko K
YK
D000818:Animals; D000830:Animals, Laboratory / Q000276:immunology*; D000914:Antibodies, Viral / Q000097:blood*; D016367:Baculoviridae / Q000276:immunology; D006224:Cricetinae / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005849:Gerbillinae / Q000276:immunology; D008216:Lymphocytic Choriomeningitis / Q000276:immunology / Q000662:veterinary*; D008217:Lymphocytic choriomeningitis virus / Q000276:immunology*; D051379:Mice; D008809:Mice, Inbred C3H / Q000276:immunology; D008813:Mice, Inbred ICR / Q000276:immunology; D051189:Murinae / Q000276:immunology; D009698:Nucleoproteins / Q000276:immunology*; D011994:Recombinant Proteins; D012377:Rodentia / Q000276:immunology*; D013047:Specific Pathogen-Free Organisms
D000914:Antibodies, Viral; D009698:Nucleoproteins; D011994:Recombinant Proteins