Characteristics of the DNA from Lymantria dispar nuclear polyhedrosis virus.
The DNA isolated from occluded Lymantria dispar nuclear polyhedrosis virus consisted of double-stranded linear, open circular, and covalently closed molecules. The DNA had a guanosine plus cytosine content (G + C) of 59-62% as estimated from both thermal denaturation and CsCl density gradient centrifugation. The L. dispar host DNA had a G + C content of 38-39%. Comparisons of molecular weight estimations for viral DNA by sedimentation on neutral sucrose gradients, electron microscopy, and reassociation kinetics gave values of 91-106 x 10(6), 94-102 x 10(6), and 78-93 x 10(6), respectively. Preliminary analysis of restriction endonuclease profiles indicated a molecular weight of approximately 88 x 10(6) for the sum of the fragments.
McCarthy
W J
WJ
Pesticide Research Laboratory and Graduate Study Center, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Murphy
T F
TF
Langridge
W
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