Thermal unfolding and aggregation of actin.
Actin is one of the most abundant proteins in nature. It is found in all eukaryotes and plays a fundamental role in many diverse and dynamic cellular processes. Also, actin is one of the most ubiquitous proteins because actin-like proteins have recently been identified in bacteria. Actin filament (F-actin) is a highly dynamic structure that can exist in different conformational states, and transitions between these states may be important in cytoskeletal dynamics and cell motility. These transitions can be modulated by various factors causing the stabilization or destabilization of actin filaments. In this review, we look at actin stabilization and destabilization as expressed by changes in the thermal stability of actin; specifically, we summarize and analyze the existing data on the thermal unfolding of actin as measured by differential scanning calorimetry. We also analyze in vitro data on the heat-induced aggregation of actin, the process that normally accompanies actin thermal denaturation. In this respect, we focus on the effects of small heat shock proteins, which can prevent the aggregation of thermally denatured actin with no effect on actin thermal unfolding. As a result, we have proposed a mechanism describing the thermal denaturation and aggregation of F-actin. This mechanism explains some of the special features of the thermal unfolding of actin filaments, including the effects of their stabilization and destabilization; it can also explain how small heat shock proteins protect the actin cytoskeleton from damage caused by the accumulation of large insoluble aggregates under heat shock conditions.
Levitsky
Dmitrii I
DI
AN Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia. levitsky@inbi.ras.ru
Pivovarova
Anastasiya V
AV
Mikhailova
Valeria V
VV
Nikolaeva
Olga P
OP
D000199:Actins / Q000737:chemistry*; D006358:Hot Temperature; D008958:Models, Molecular; D010590:Phalloidine / Q000737:chemistry; D011489:Protein Denaturation
D000199:Actins; D010590:Phalloidine