The association of host and phage DNA with the membrane of Escherichia coli.
The attachment of DNA to membrane in T4-infected cells was investigated by means of the Mg2+-sarkosyl crystals technique. Both parental and progeny phage DNA are associated with cell membrane throughout the eclipse period and are subsequently detached during the synthesis of mature phage. The initial attachment process does not require protein synthesis. Detachment appears to be due to a "late function" since it does not occur following infection with a DO mutant (amN82) or after treatment with chloramphenicol or tetracycline added 9 min after infection. Host DNA remains attached to membrane during the initial stages of infection and neither superinfection nor the addition of chloramphenicol alters this association.
Earhart
C F
CF
Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.