title: Improving digestibility of soy flour by reducing disulfide bonds with thioredoxin.
abstract: The Kunitz trypsin inhibitor (KTI) and the Bowman-Birk inhibitor (BBI) of trypsin and chymotrypsin contain disulfide bonds. Glycinin, the major storage protein in soybeans also contains disulfide bonds. Treatment of soy white flour with a NADP-thioredoxin system (NTS) effectively reduced disulfide bonds in soy flour and increased protein digestibility by trypsin and pancreatin as measured by the pH stat method. Treatment of soy flour with NTS increased the digestibility compared to soy white flour by 29.3 and 60.6% for trypsin and pancreatin, respectively. NTS-treated soy flour had similar digestibility by trypsin to autoclaved soy flour and casein, but digestibility by pancreatin was less than autoclaved soy flour and casein. The degree of reduction by NTS was highly correlated to the degree of hydrolysis (DH) by trypsin (R(2) = 0.93) and pancreatin (R(2) = 0.99). The DH of NTS-treated soy flour by trypsin is reflective of both inactivation of trypsin inhibitors and overall protein digestibility while pancreatin hydrolysis is reflective of only overall protein digestibility.
authors.affiliation: Department of Animal Science, Iowa State University, 1221 Kildee Hall, Ames, Iowa 50011, USA.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Faris
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.forename: Tong
authors.initials: T
authors.lastname: Wang
mesh_terms: D004063:Digestion; D004220:Disulfides / Q000737:chemistry*; D005511:Food Handling; D009249:NADP / Q000737:chemistry; D010084:Oxidation-Reduction; D010194:Pancreatin / Q000378:metabolism; D030262:Soybean Proteins / Q000378:metabolism*; D013025:Glycine max / Q000737:chemistry*; D013438:Sulfhydryl Compounds / Q000032:analysis; D013879:Thioredoxins / Q000737:chemistry*; D014357:Trypsin / Q000378:metabolism
chemical_list: D004220:Disulfides; D030262:Soybean Proteins; D013438:Sulfhydryl Compounds; D013879:Thioredoxins; D009249:NADP; D010194:Pancreatin; D014357:Trypsin