title: [Odontoblast-like cell phenotype differentiation of cranial neural crest stem cell in vivo].
abstract: OBJECTIVE
  To investigate the feasibility of tooth regeneration by seeding cranial neural crest stem cell (CNCSC) in vivo.
  
  
  METHODS
  Cranial neural tubes, dissected from mouse E9 d, were explanted onto fibronectin-coated dishes. CNCSC emigrated from the explanted neural tubes, and were cultured in a free-serum medium containing modified DMEM/F12. CNCSC, induced by FGF8, BMP2, TGFbeta1 and dentin matrix non-collagen protein (DMNCP), were cultured with collagen/chitosan, and implanted into the subcutaneous part of immunodeficiency mouse. The expression of collagen I/dentin sialophosphoprotein (DSPP) was analyzed by immunocytochemistry.
  
  
  RESULTS
  With the scaffolds destroying, columnar cells possessing polarized nuclei and matrix produced by cells were showed in some regions. Immunohistochemical staining demonstrated that collagen type I and DSPP were expressed throughout the cytoplasm and matrix produced by cells.
  
  
  CONCLUSIONS
  By tissue engineering approach, our experiments further verify the odontoblast-like cell phenotype differentiation of CNCSC in vivo.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Hong-Bing
authors.initials: HB
authors.lastname: Jiang
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Tian
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Zheng
authors.forename: Xi-Zhe
authors.initials: XZ
authors.lastname: Chen
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D024042:Collagen Type I / Q000032:analysis; D003391:Cranial Nerves / Q000737:chemistry / Q000166:cytology; D016326:Extracellular Matrix Proteins; D007150:Immunohistochemistry; D051379:Mice; D008819:Mice, Nude; D009432:Neural Crest / Q000737:chemistry / Q000166:cytology*; D009804:Odontoblasts / Q000737:chemistry / Q000166:cytology*; D010750:Phosphoproteins; D019736:Prostheses and Implants; D011498:Protein Precursors / Q000032:analysis; D012038:Regeneration; D012795:Sialoglycoproteins; D033581:Stem Cell Transplantation / Q000379:methods; D013234:Stem Cells / Q000737:chemistry / Q000166:cytology*; D014070:Tooth / Q000737:chemistry / Q000254:growth & development / Q000502:physiology; D000099311:Dentin Sialophosphoprotein
chemical_list: D024042:Collagen Type I; D016326:Extracellular Matrix Proteins; D010750:Phosphoproteins; D011498:Protein Precursors; D012795:Sialoglycoproteins; D000099311:Dentin Sialophosphoprotein