title: Influence of fluoroquinolones on phagocytosis and killing of Candida albicans by human polymorphonuclear neutrophils.
abstract: Candida albicans infections often occur during or shortly after antibacterial treatment. Phagocytosis by polymorphonuclear neutrophil granulocytes (PMN) is the most important primarily defence mechanism against C. albicans. Certain antibiotics such as some fluoroquinolones (FQ) are known to influence phagocyte functions. Thus, we investigated the influence of older and newer FQ on the phagocytosis and killing of C. albicans by human PMN paying special attention to CD11b expression of these cells as an indicator of the degree of their activation. In order to obtain comprehensive and comparable results we tested 13 FQ over a wide range of concentrations and in a time dependent manner in a standardized approach. When used at therapeutic concentrations, the FQ tested did not influence to a clinically significant degree the phagocytosis or the killing of C. albicans by human PMN and also not their activation. However, at high concentrations those FQ with cyclopropyl-moiety at position N1 showed increase in CD11b expression and diminished phagocytosis and oxidative burst.
authors.affiliation: Federal Institute for Drugs and Medical Devices (BfArM), Biosafety Laboratory, Bonn, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Gruger
authors.forename: Caroline
authors.initials: C
authors.lastname: Morler
authors.forename: Norbert
authors.initials: N
authors.lastname: Schnitzler
authors.forename: Kerstin
authors.initials: K
authors.lastname: Brandenburg
authors.forename: Sabine
authors.initials: S
authors.lastname: Nidermajer
authors.forename: Regine
authors.initials: R
authors.lastname: Horre
authors.forename: Josef
authors.initials: J
authors.lastname: Zundorf
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D039481:CD11b Antigen / Q000378:metabolism; D002176:Candida albicans / Q000187:drug effects / Q000502:physiology*; D005260:Female; D024841:Fluoroquinolones / Q000494:pharmacology*; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D008875:Middle Aged; D009504:Neutrophils / Q000187:drug effects*; D010587:Phagocytosis / Q000187:drug effects*; D016897:Respiratory Burst / Q000187:drug effects; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D039481:CD11b Antigen; D024841:Fluoroquinolones