title: Defect in TCR-CD3zeta signaling mediates T cell hypo-responsiveness in mesenteric lymph node.
abstract: Mesenteric lymph node (MLN) in gut-associated lymphoid tissue plays obligatory roles in the induction of oral tolerance and ignorance to commensals. However, little is known about its immunological characteristics. In this study, we investigated the hypo-responsiveness of MLN CD4(+) T cells, comparing them with spleen CD4(+) T cells. MLN CD4(+) T cells were hypo-proliferative and expressed low levels of Th1-type cytokines in response to antigen or CD3/T cell receptor (TCR) stimulation. The hypo-responsiveness of MLN CD4(+) T cells is linked neither with changes in the regulatory T cell population (CD4(+)CD25(+), CD4(+)Foxp3(+)) nor the apoptotic population. Rather, MLN CD4(+) T cells showed deformity of T cell:APC conjugation and reduced expression of TCR signaling molecules such as CD3zeta, PLC-gamma1, PKC-theta, Zap70, with reduced phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs). Among the alterations in TCR signaling molecules, defective CD3zeta expression is the most evident, and reversal of the anergic state by CD3/CD28 costimulation restored CD3zeta expression levels. Collectively, we suggest that reduced CD3zeta expression and defects in TCR signaling mediate the anergy state of MLN CD4(+) T cells, which play a critical role in maintenance of mucosal tolerance in gut-associated lymphoid tissue.
authors.affiliation: Department of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, Republic of Korea.
authors.forename: Hwa-Jung
authors.initials: HJ
authors.lastname: Yi
authors.forename: Choong-Gu
authors.initials: CG
authors.lastname: Lee
authors.forename: Ho-Keun
authors.initials: HK
authors.lastname: Kwon
authors.forename: Jae-Seon
authors.initials: JS
authors.lastname: So
authors.forename: Anupama
authors.initials: A
authors.lastname: Sahoo
authors.forename: Ji-Sun
authors.initials: JS
authors.lastname: Hwang
authors.forename: Arijita
authors.initials: A
authors.lastname: Jash
authors.forename: Ki-Chul
authors.initials: KC
authors.lastname: Hwang
authors.forename: Sin-Hyeog
authors.initials: SH
authors.lastname: Im
mesh_terms: D000818:Animals; D017252:CD3 Complex / Q000276:immunology*; D008198:Lymph Nodes / Q000276:immunology*; D008643:Mesentery / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D011948:Receptors, Antigen, T-Cell / Q000276:immunology*; D015398:Signal Transduction / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology*
chemical_list: D017252:CD3 Complex; C121704:CD3 antigen, zeta chain; D011948:Receptors, Antigen, T-Cell