title: New options in assisted reproduction technology: the Cryotop method of oocyte vitrification.
abstract: The Cryotop vitrification method has been shown to be a very useful tool for oocyte cryopreservation, giving excellent results regarding survival and clinical outcome. There are several clinical situations in which oocyte cryopreservation provides solutions that have not been available to date. This report describes three of these situations: (i) a low-responder patient who needed a single gene diagnosis due to the presence of a genetic disease; (ii) a patient undergoing endometrial bleeding on the day of oocyte retrieval who was also affected by a genetic disorder; and (iii) a patient who failed to become pregnant after the donation of vitrified oocytes and subsequently had the re-vitrified surplus embryos transferred. The resolution of these cases provides evidence of the enormous potential of the Cryotop method as a tool within assisted reproduction technology.
authors.affiliation: IVI Universidad de Valencia, Plaza de la Policía local, 3 46015 Valencia, Spain. acobo@ivi.es
authors.forename: Ana
authors.initials: A
authors.lastname: Cobo
authors.forename: José
authors.initials: J
authors.lastname: Bellver
authors.forename: Javier
authors.initials: J
authors.lastname: Domingo
authors.forename: Sonia
authors.initials: S
authors.lastname: Pérez
authors.forename: Juana
authors.initials: J
authors.lastname: Crespo
authors.forename: Antonio
authors.initials: A
authors.lastname: Pellicer
authors.forename: José
authors.initials: J
authors.lastname: Remohí
mesh_terms: D000328:Adult; D015925:Cryopreservation / Q000295:instrumentation / Q000379:methods*; D046149:Embryo Culture Techniques; D004717:Endometrium / Q000473:pathology; D005260:Female; D006801:Humans; D006816:Huntington Disease / Q000235:genetics; D008297:Male; D009865:Oocytes / Q000166:cytology*; D011085:Polycystic Ovary Syndrome / Q000235:genetics; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D011256:Pregnancy Outcome; D019836:Preimplantation Diagnosis / Q000379:methods; D027724:Reproductive Techniques, Assisted