title: Effect of an ethanol/trisodium citrate hemodialysis catheter locking solution on isolates of Candida albicans.
abstract: We conducted an in vitro study to assess the effect of a 30% ethanol/4% trisodium citrate (TSC) catheter locking solution on isolates of Candida albicans. Twelve isolates obtained from human blood cultures were tested in control solutions composed of broth and normal saline, and a test solution of 30% ethanol, 4% TSC, and broth. Colony counts were determined for control and test solutions at baseline and after 1, 24, and 48 hours of exposure. After 48 hours, the remaining test solution was filtered through a sterile filter funnel and rinsed with 10 mL of sterile water. Filters were aseptically transferred to agar plates and incubated for 24 hours. Control solutions grew well over the incubation period, as expected. In contrast, no viable growth was observed in test solutions 1 hour after instillation of the ethanol/TSC locking solution, or throughout the 48-hour study duration. Additionally, filters from test solutions displayed no growth. We conclude that a 30% ethanol/4% TSC catheter locking solution eradicated all isolates of C. albicans within 1 hour of exposure in this in vitro study, and shows promise as a new hemodialysis catheter locking solution.
authors.affiliation: Faculty of Pharmacy, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Anil R
authors.initials: AR
authors.lastname: Maharaj
authors.forename: Sheryl A
authors.initials: SA
authors.lastname: Zelenitsky
authors.forename: Lavern M
authors.initials: LM
authors.lastname: Vercaigne
mesh_terms: D000925:Anticoagulants / Q000494:pharmacology; D002176:Candida albicans / Q000187:drug effects* / Q000254:growth & development*; D002177:Candidiasis / Q000517:prevention & control*; D002408:Catheters, Indwelling / Q000382:microbiology; D002951:Citrates / Q000494:pharmacology*; D015169:Colony Count, Microbial; D000431:Ethanol / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D016459:Prosthesis-Related Infections / Q000382:microbiology / Q000517:prevention & control; D006435:Renal Dialysis
chemical_list: D000925:Anticoagulants; D002951:Citrates; C514290:trisodium citrate; D000431:Ethanol