title: Physico-chemical properties of the encapsulation matrix and germination of carrot somatic embryos.
abstract: Carrot somatic embryos were encapsulated in alginate gel beads. To improve the quality of a "synthetic seed" coating, the rheology and dehydration properties of different matrices were tested. By increasing alginate and CaCl(2) concentrations, additional mineral elements were shown to increase resistance to rupture, and to depress the germination of somatic embryos. A polysaccharide addition was found to slow the alginate matrix dehydration; alginate-gellan gum and alginate-kaolin matrices could preserve the viability of somatic embryos at low relative humidities (30% to 35% germinations at 50% relative humidity) to a greater extent than other matrices.
authors.affiliation: Laboratoire de Technologie Enzymatique, URA 1442 du CNRS, Université de Technologie de Compiègne BP 649, 60200 Compiègne, France.
authors.forename: R
authors.initials: R
authors.lastname: Timbert
authors.forename: J N
authors.initials: JN
authors.lastname: Barbotin
authors.forename: A
authors.initials: A
authors.lastname: Kersulec
authors.forename: C
authors.initials: C
authors.lastname: Bazinet
authors.forename: D
authors.initials: D
authors.lastname: Thomas