[Effect of scalp-acupuncture on energy metabolism of brain tissue in intracerebral hemorrhage rats].
OBJECTIVE
To observe the effect of electroacupuncture (EA) on mitochondrial SDH and Na+ -K+ -ATPase activity, and lactate acid (LA) content in the peri-hemotoma tissue in rats with intracerebral hemorrhage (ICH) so as to explore its mechanisms in resisting cerebral ischemia.


METHODS
A total of 80 Wistar rats were randomized into normal group, sham group, model group, EA group. The last three groups were further divided into 24 h, 72 h and 168 h subgroups respectively, with 8 cases in each group/subgroups. ICH model was established by caudatum-injection (right) of Collagense VII/heparini solution (2 microl). EA (2 Hz, 1 mA) was applied to "Baihui" (GV 20)-"Taiyang" (EX-HN 5) for 30 min. Chondriosome suspension of the cerebral perihemotoma tissue was got by gradient centrifugation for detecting SDH and Na+ -K+ -ATPase activity, and LA content with chromometry.


RESULTS
In comparison with sham-operation group and control group, SDH and mitochondrial Na+ -K+ -ATPase activity in cerebral peri-hemotoma tissue declined significantly from 72 h and 24 h on respectively (P<0.05), while LA level increased markedly from 24 h on (P<0.05) in model group. Compared with sham-operation group, SDH activity (168 h) and Na+ -K+ -ATPase activity (24 h, 72 h and 168 h) in EA group also decreased significantly (P<0.05); LA level (24 h, 72 h) in EA group increased considerably (P<0.05). Whereas compared with model group, SDH and Na+ -K+ -ATPase activity increased significantly at most time courses (P<0.05), and LA level decreased remarkably (P<0.05) in EA group.


CONCLUSIONS
EA can improve the energy metabolism, upregulate the activity of chondriosome,and reduce the accumulation of LA in the cerebral perihemotoma tissue of ICH rats.
Bao
Chun-Ling
CL
Yueyang Hospital of Shanghai University of Chinese Medicine, Shanghai 200437, China. chunlingb@sina.com
Dong
Hong-Sheng
HS
D026881:Acupuncture; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002543:Cerebral Hemorrhage / Q000378:metabolism / Q000628:therapy*; D004734:Energy Metabolism; D019344:Lactic Acid / Q000032:analysis / Q000378:metabolism; D008297:Male; D008928:Mitochondria / Q000201:enzymology; D051381:Rats; D017208:Rats, Wistar; D012535:Scalp; D000254:Sodium-Potassium-Exchanging ATPase / Q000378:metabolism; D013385:Succinate Dehydrogenase / Q000378:metabolism
D019344:Lactic Acid; D013385:Succinate Dehydrogenase; D000254:Sodium-Potassium-Exchanging ATPase