Probing the effect of the binding site on the electrostatic behavior of a series of carotenoids reconstituted into the light-harvesting 1 complex from purple photosynthetic bacterium Rhodospirillum rubrum detected by stark spectroscopy.
Reconstitutions of the LH1 complexes from the purple photosynthetic bacterium Rhodospirillum rubrum S1 were performed with a range of carotenoid molecules having different numbers of C=C conjugated double bonds. Since, as we showed previously, some of the added carotenoids tended to aggregate and then to remain with the reconstituted LH1 complexes (Nakagawa, K.; Suzuki, S.; Fujii, R.; Gardiner, A.T.; Cogdell, R.J.; Nango, M.; Hashimoto, H. Photosynth. Res. 2008, 95, 339-344), a further purification step using a sucrose density gradient centrifugation was introduced to improve purity of the final reconstituted sample. The measured absorption, fluorescence-excitation, and Stark spectra of the LH1 complex reconstituted with spirilloxanthin were identical with those obtained with the native, spirilloxanthin-containing, LH1 complex of Rs. rubrum S1. This shows that the electrostatic environments surrounding the carotenoid and bacteriochlorophyll a (BChl a) molecules in both of these LH1 complexes were essentially the same. In the LH1 complexes reconstituted with either rhodopin or spheroidene, however, the wavelength maximum at the BChl a Qy absorption band was slightly different to that of the native LH1 complexes. These differences in the transition energy of the BChl a Qy absorption band can be explained using the values of the nonlinear optical parameters of this absorption band, i.e., the polarizability change Tr(Deltaalpha) and the static dipole-moment change |Deltamu| upon photoexcitation, as determined using Stark spectroscopy. The local electric field around the BChl a in the native LH1 complex (ES) was determined to be approximately 3.0x10(6) V/cm. Furthermore, on the basis of the values of the nonlinear optical parameters of the carotenoids in the reconstituted LH1 complexes, it is possible to suggest that the conformations of carotenoids, anhydrorhodovibrin and spheroidene, in the LH1 complex were similar to that of rhodopin glucoside in crystal structure of the LH2 complex from Rhodopseudomonas acidophila 10050.
Nakagawa
Katsunori
K
Department of Life and Materials Engineering, Graduate School of Engineering, Nagoya Institute of Technology, Nagoya, Japan.
Suzuki
Satoru
S
Fujii
Ritsuko
R
Gardiner
Alastair T
AT
Cogdell
Richard J
RJ
Nango
Mamoru
M
Hashimoto
Hideki
H
D001426:Bacterial Proteins / Q000378:metabolism*; D001665:Binding Sites; D002338:Carotenoids / Q000737:chemistry* / Q000378:metabolism*; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D008958:Models, Molecular; D015394:Molecular Structure; D017711:Nonlinear Dynamics; D010788:Photosynthesis; D012247:Rhodospirillum rubrum / Q000201:enzymology; D013053:Spectrophotometry; D013057:Spectrum Analysis; D055672:Static Electricity
D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; C094744:light-harvesting complex 1, Rhodospirillum; D002338:Carotenoids