Genetic engineering to contain the Vitreoscilla hemoglobin gene enhances degradation of benzoic acid by Xanthomonas maltophilia.
Xanthomonas maltophilia was transformed with the gene encoding Vitreoscilla (bacterial) hemoglobin, vgb, and the growth of the engineered strain was compared with that of the untransformed strain using benzoic acid as the sole carbon source. In general, growth of the engineered strain was greater than that of the untransformed strain; this was true for experiments using both overnight cultures and log phase cells as inocula, but particularly for the latter. In both cases the engineered strain was also more efficient than the untransformed strain in converting benzoic acid into biomass.
Liu
S C
SC
Division of Biology, Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology IIT Center, Chicago, Illinois 60616, USA.
Webster
D A
DA
Wei
M L
ML
Stark
B C
BC