title: Cerebrospinal fluid Diacron-Reactive Oxygen Metabolite levels in pediatric patients with central nervous system diseases.
abstract: This study assessed the validity of cerebrospinal fluid oxidative status of pediatric patients with central nervous system diseases, using the Diacron-Reactive Oxygen Metabolites test (d-Roms; Diacron International s.r.l.; Grosseto, Italy). Eighty-seven pediatric patients (8 with bacterial meningitis, 18 with aseptic meningitis, 23 with febrile seizures, 6 with rotavirus gastroenteritis-induced convulsions, 16 with epilepsy, 2 with adrenoleukodystrophy, 2 with multiple sclerosis, and 12 control subjects) were enrolled. An analysis of the infection-associated group (bacterial meningitis, aseptic meningitis, febrile seizures, and rotavirus gastroenteritis-induced convulsions) indicated that cerebrospinal fluid Diacron-Reactive Oxygen Metabolite levels in the bacterial meningitis group were significantly higher than in other infection-associated groups. In the bacterial meningitis group, the cerebrospinal fluid Diacron-Reactive Oxygen Metabolite levels obtained after improvement were significantly decreased compared with pre-improvement values. In the noninfection-associated group (epilepsy, adrenoleukodystrophy, and multiple sclerosis), the cerebrospinal fluid Diacron-Reactive Oxygen Metabolite levels in symptomatic epilepsy patients were higher than in cryptogenic epilepsy patients and control subjects, but not significantly. Progressive patients with adrenoleukodystrophy or multiple sclerosis demonstrated high Diacron-Reactive Oxygen Metabolite levels compared with another early-stage adrenoleukodystrophy patient. Oxidative stress may be associated with the pathogenesis of various pediatric central nervous system diseases. Cerebrospinal fluid Diacron-Reactive Oxygen Metabolite levels may correlate with clinical status in these diseases.
authors.affiliation: Department of Pediatrics, Tokyo Medical University, Shinjuku, Tokyo, Japan. gaku@tokyo-med.ac.jp
authors.forename: Gaku
authors.initials: G
authors.lastname: Yamanaka
authors.forename: Chiako
authors.initials: C
authors.lastname: Ishii
authors.forename: Hisashi
authors.initials: H
authors.lastname: Kawashima
authors.forename: Shingo
authors.initials: S
authors.lastname: Oana
authors.forename: Tasuku
authors.initials: T
authors.lastname: Miyajima
authors.forename: Akinori
authors.initials: A
authors.lastname: Hoshika
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002493:Central Nervous System Diseases / Q000134:cerebrospinal fluid* / Q000145:classification / Q000628:therapy; D002648:Child; D002675:Child, Preschool; D005260:Female; D005609:Free Radicals / Q000097:blood / Q000134:cerebrospinal fluid*; D006801:Humans; D007223:Infant; D008297:Male; D008582:Meningitis, Aseptic / Q000134:cerebrospinal fluid / Q000150:complications; D016920:Meningitis, Bacterial / Q000134:cerebrospinal fluid / Q000150:complications; D008875:Middle Aged; D010372:Pediatrics; D017382:Reactive Oxygen Species / Q000097:blood / Q000134:cerebrospinal fluid*; D018709:Statistics, Nonparametric
chemical_list: D005609:Free Radicals; D017382:Reactive Oxygen Species