title: Zinc-mediated feedback at the synaptic terminals of vertebrate photoreceptors.
abstract: There is mounting evidence that zinc release from glutamatergic nerve terminals serves as a neuromodulator at synaptic sites within the retina and CNS. However, it has not been possible to reliably measure the concentration of zinc co-released with glutamate in the confines of the synaptic cleft. Thus, much of the evidence supporting this view derives from electrophysiological studies showing the modulatory effects of exogenous zinc on the membrane currents of ligand- and voltage-gated channels. In the present study, we took advantage of the unique properties of the glutamatergic photoreceptor terminal to demonstrate a feedback signal mediated by endogenous zinc at the synaptic sites from which it is discharged. Through its ability to block voltage-gated calcium channels in the photoreceptor terminal, zinc suppresses the radial dark current of the visual cell, and reduces its release of glutamate. It follows that chelation of extracellular zinc, e.g., with histidine, will lead to an increase both in the dark current and in the release of glutamate, changes that result in an enhancement of the light-evoked a-wave of the ERG and can account for the b-wave enhancement observed previously after zinc chelation when inner retinal responses were not blocked by aspartate.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Chappell
authors.forename: Ivan
authors.initials: I
authors.lastname: Anastassov
authors.forename: Prospero
authors.initials: P
authors.lastname: Lugo
authors.forename: Harris
authors.initials: H
authors.lastname: Ripps
mesh_terms: D000818:Animals; D004596:Electroretinography; D025461:Feedback, Physiological / Q000502:physiology; D017729:Presynaptic Terminals / Q000378:metabolism*; D017948:Retinal Rod Photoreceptor Cells / Q000378:metabolism*; D012860:Skates, Fish; D015032:Zinc / Q000502:physiology*
chemical_list: D015032:Zinc