title: [The fabrication of hepatitis B electrochemical immunosensor array].
abstract: Based on integrate circuit (IC) technology, an eight-channel gold electrodes (GEs) array was developed. The immunosensor array is prepared by co-immobilizing thionine and Hepatitis B (HB) antibody on the gold electrodes through covalently binding them to GEs with a cysteamine/glutaraldehyde linkage. Hepatitis B surface antigen (HBsAg) was detected qualitatively and quantitatively by the peak current decrease percentage of the thionine. HBsAg positive/negative standard serum was well defined by the array. 8-channel synchronous detection for HBsAg was noted to be of good accuracy and reliability. The results of its clinical application were in good agreement with the results from ELISA.
authors.affiliation: General Hospital of Guangzhou Command, Guangzhou 510010, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Dai
authors.forename: Zhongming
authors.initials: Z
authors.lastname: Liu
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D017097:Electric Impedance; D004563:Electrochemistry / Q000295:instrumentation* / Q000379:methods; D004566:Electrodes; D006510:Hepatitis B Antibodies / Q000276:immunology; D006514:Hepatitis B Surface Antigens / Q000032:analysis* / Q000276:immunology; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens