Global expression profiling identifies a novel biosignature for protein aggregation R120GCryAB cardiomyopathy in mice.
Protein aggregation cardiomyopathy is a life-threatening manifestation of a multisystem disorder caused by the exchange mutation in the gene encoding the human small heat shock protein alphaB-crystallin (hR120GCryAB). Genetic studies in mice have established cardiac hR120GCryAB expression causes increased activity of glucose 6-phosphate dehydrogenase (G6PD) and "reductive stress" (Rajasekaran et al., Cell 130: 427-439, 2007). However, the initiating molecular events in the pathogenesis of this novel toxic gain-of-function mechanism remain poorly defined. In an integrated systems approach using gene expression profiling, we identified a "biosignature," whose features can be validated to predict the onset, rate of progression, and clinical outcome of R120GCryAB cardiomyopathy. At the 3 mo disease-related but compensated stage, we demonstrate that transcripts were only upregulated in three distinct pathways: stress response (e.g., Hsp70, Hsp90), glutathione metabolism (Gpx1, Gpx3, glutathione S-transferase), and complement and coagulation cascades in hR120GCryAB transgenic mouse hearts compared with either hCryAB WT transgenic mice or nontransgenic controls. In 6 mo old myopathic hearts, ribosomal synthesis and cellular remodeling associated with increased cardiac hypertrophy were additional upregulated pathways. In contrast, the predominant downregulated pathways were for oxidative phosphorylation, fatty acid metabolism, intermediate metabolism, and energetic balance, supporting their primary pathogenic roles by which G6PD-dependent reductive stress causes cardiac decompensation and overt heart failure in hR120GCryAB cardiomyopathy. This study extends and confirms our previous findings that reductive stress is a causal mechanism for hR120G CryAB cardiomyopathy and demonstrates that alteration in glutathione pathway gene expression is an early biosignature with utility for presymptomatic detection.
Rajasekaran
Namakkal S
NS
Departments of Internal Medicine, Division of Cardiology, Center for Cardiovascular Translational Biomedicine, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
Firpo
Matthew A
MA
Milash
Brett A
BA
Weiss
Robert B
RB
Benjamin
Ivor J
IJ
D000818:Animals; D001120:Arginine / Q000235:genetics*; D009202:Cardiomyopathies / Q000235:genetics* / Q000378:metabolism*; D020869:Gene Expression Profiling; D005978:Glutathione / Q000235:genetics / Q000378:metabolism; D005998:Glycine / Q000235:genetics*; D006801:Humans; D051379:Mice; D008822:Mice, Transgenic; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis; D038203:alpha-Crystallin B Chain / Q000235:genetics*
D038203:alpha-Crystallin B Chain; D001120:Arginine; D005978:Glutathione; D005998:Glycine