title: Identification of osteo-adipo progenitor cells in fat tissue.
abstract: OBJECTIVE
  In this study, a group of cells that expressed both osteogenic and adipogenic characters was identified from murine adipose stromal cells.
  
  
  METHODS
  These cells could be enriched in the Sca-1-1 population and express both osteogenic and adipogenic genes. Osteogenic induction enhanced expression of osteogenic genes and inhibited expression of adipogenic genes, while adipogenic induction enhanced expression of adipogenic genes and inhibited expression of osteogenic genes. These cells have been called osteo-adipo progenitors (OAPs).
  
  
  RESULTS
  OAPs expressed transcription factor runt-related transcription factor 2 (RUNX2) and peroxisome proliferator-activated receptor-gamma (PPAR-gamma) proteins in cytoplasm. When OAPs were cultured in adipogenic medium, PPAR-gamma moved to the nucleus and the cells differentiated into adipocytes, while the RUNX2 remained in the cytoplasm. In contrast, when OAPs were cultured in osteogenic medium, RUNX2 moved to the nucleus and the cells differentiated to osteocytes, while the PPAR-gamma remained in the cytoplasm.
  
  
  CONCLUSIONS
  These experiments suggest that osteoblasts and adipocytes share a common predecessor, the OAP, in murine adipose stromal cells.
authors.affiliation: State Key Laboratory of Oral Diseases, Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu, China.
authors.forename: Y F
authors.initials: YF
authors.lastname: Lin
authors.forename: W
authors.initials: W
authors.lastname: Jing
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.forename: Y
authors.initials: Y
authors.lastname: Wu
authors.forename: L
authors.initials: L
authors.lastname: Liu
authors.forename: W
authors.initials: W
authors.lastname: Tang
authors.forename: J
authors.initials: J
authors.lastname: Long
authors.forename: W D
authors.initials: WD
authors.lastname: Tian
authors.forename: X M
authors.initials: XM
authors.lastname: Mo
mesh_terms: D017667:Adipocytes / Q000166:cytology*; D050156:Adipogenesis / Q000235:genetics; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D000067696:Ataxin-1; D000067528:Ataxins; D002454:Cell Differentiation; D019070:Cell Lineage; D002465:Cell Movement; D002469:Cell Separation; D003470:Culture Media; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D005786:Gene Expression Regulation; D051379:Mice; D009419:Nerve Tissue Proteins / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D010011:Osteocytes / Q000166:cytology*; D010012:Osteogenesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D017154:Stromal Cells / Q000166:cytology
chemical_list: D000067696:Ataxin-1; D000067528:Ataxins; C000593750:Atxn1 protein, mouse; D003470:Culture Media; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins