Gallera method of chick embryo culture in vitro supports better growth compared with original New method.
An avian embryo is a valuable model system for vertebrate embryology. Easy availability, accessibility to various developmental stages and amenability of organ fields makes the chick embryo one of the favored model systems. Seminal discoveries regarding organogenesis and vertebrate morphogenesis have been made using chick embryos cultured in vitro. Dennis A.T. New revolutionized chick embryo culture methodology with his development of a single glass ring explantation technique. Many modifications and/or embellishments were introduced after the New era of embryo culture. A double glass ring method for chick embryo culture introduced by Gallera and Nicolet is compared with the original New method and the EASY method in this study. In addition, a video of culture methods is presented as a valuable tool in learning about and/or teaching techniques of chick embryo culture.
Ghatpande
Satish K
SK
Department of Pediatrics, Section of Developmental Endocrinology and Biology, Yale Child Health Research Center, Yale University School of Medicine, New Haven, Connecticut 06520, USA. satish.ghatpande@yale.edu
D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002642:Chick Embryo; D015509:Developmental Biology / Q000266:history* / Q000379:methods*; D046149:Embryo Culture Techniques / Q000379:methods*; D004625:Embryo, Nonmammalian; D049673:History, 20th Century; D049674:History, 21st Century; D013997:Time Factors