Application of quantitative gene expression analysis for pertussis vaccine safety control.
Although vaccines are routinely used to prevent infectious diseases, little is known about the comprehensive influences caused by vaccines. In this study, we showed, using comprehensive gene expression analysis, that pertussis vaccine affected many genes in multiple organs of vaccine-treated animals. In particular, lung was revealed to be the most suitable target to evaluate pertussis vaccine toxicity. The 13 genes identified from the analysis of vaccine-treated lung at day 1 showed a clear dendrogram corresponding to pertussis vaccine toxicity. Furthermore, quantitative analysis of these genes revealed a positive correlation between their respective expression levels and the degree of toxic effects observed in samples that had been treated with various doses of reference pertussis vaccines. The quantification of this 13 gene-set is an indicator of the vaccine toxicity-related reaction.
Hamaguchi
Isao
I
Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
Imai
Jun-ichi
J
Momose
Haruka
H
Kawamura
Mika
M
Mizukami
Takuo
T
Naito
Seishiro
S
Maeyama
Jun-ichi
J
Masumi
Atsuko
A
Kuramitsu
Madoka
M
Takizawa
Kazuya
K
Kato
Hiroshi
H
Mizutani
Tetsuya
T
Horiuchi
Yoshinobu
Y
Nomura
Nobuo
N
Watanabe
Shinya
S
Yamaguchi
Kazunari
K
D000818:Animals; D001835:Body Weight; D020869:Gene Expression Profiling / Q000379:methods*; D008168:Lung / Q000187:drug effects; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010567:Pertussis Vaccine / Q000009:adverse effects* / Q000633:toxicity*; D051381:Rats; D017208:Rats, Wistar; D015854:Up-Regulation
D010567:Pertussis Vaccine