title: Production and characterization of a plant alpha-hydroxynitrile lyase in Escherichia coli.
abstract: The coding sequence of the cyanogenic alpha-hydroxynitrile lyase gene of Manihot esculenta Crantz (cassava) was cloned in the plasmid vector pMal-c2 and expressed in Escherichia coli strain JM105. DNA sequencing showed that the recombinant plasmid contained the same sequence as the cDNA clone pHNL10. Peptide sequencing of the recombinant protein showed that the N-terminus was heterogeneous, with either four or six additional amino acid residues compared with the native protein. Circular dichroism spectra indicated similar secondary structure contents for both proteins. Enzyme assays showed that specific activity of native and recombinant proteins were 0.24 and 0.26 mmol CN(-)/mg/min, respectively; that both proteins had optimal activity at 40 degrees C and pH 5.5; and that both proteins were inhibited by the serine protease inhibitor phenyl-methane sulfonyl flouride (PMSF). Isoelectric focusing of native and recombinant protein revealed multiple isoforms for both proteins; the recombinant protein had a more basic mean isoelectric point (pl) (5.1) than the native protein (4.5).
authors.affiliation: Department of Biochemistry and Genetics, University of Newcastle upon Tyne, Newcastle upon Tyne NE2 4HH, United Kingdom. m.a.hughes@ncl.ac.uk
authors.forename: J
authors.initials: J
authors.lastname: Hughes
authors.forename: J H
authors.initials: JH
authors.lastname: Lakey
authors.forename: M A
authors.initials: MA
authors.lastname: Hughes