Metabonomics investigation of human urine after ingestion of green tea with gas chromatography/mass spectrometry, liquid chromatography/mass spectrometry and (1)H NMR spectroscopy.
A method using gas chromatography/mass spectrometry (GC/MS), liquid chromatography/mass spectrometry (LC/MS) and (1)H NMR with pattern recognition tools such as principle components analysis (PCA) was used to study the human urinary metabolic profiles after the intake of green tea. From the normalized peak areas obtained from GC/MS and LC/MS and peak heights from (1)H NMR, statistical analyses were used in the identification of potential biomarkers. Metabolic profiling by GC/MS provided a different set of quantitative signatures of metabolites that can be used to characterize the molecular changes in human urine samples. A comparison of normalized metabonomics data for selected metabolites in human urine samples in the presence of potential overlapping peaks after tea ingestion from LC/MS and (1)H NMR showed the reliability of the current approach and method of normalization. The close agreements of LC/MS with (1)H NMR data showed that the effects of ion suppression in LC/MS for early eluting metabolites were not significant. Concurrently, the specificity of detecting the stated metabolites by (1)H NMR and LC/MS was demonstrated. Our data showed that a number of metabolites involved in glucose metabolism, citric acid cycle and amino acid metabolism were affected immediately after the intake of green tea. The proposed approach provided a more comprehensive picture of the metabolic changes after intake of green tea in human urine. The multiple analytical approach together with pattern recognition tools is a useful platform to study metabolic profiles after ingestion of botanicals and medicinal plants.
Law
Wai Siang
WS
Department of Chemistry, National University of Singapore, 3 Science Drive 3, Republic of Singapore 117543.
Huang
Pei Yun
PY
Ong
Eng Shi
ES
Ong
Choon Nam
CN
Li
Sam Fong Yau
SF
Pasikanti
Kishore Kumar
KK
Chan
Eric Chun Yong
EC
D000328:Adult; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D008297:Male; D008660:Metabolism; D010936:Plant Extracts / Q000032:analysis / Q000493:pharmacokinetics*; D025341:Principal Component Analysis; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013662:Tea / Q000378:metabolism*; D016482:Urinalysis / Q000379:methods*
D015415:Biomarkers; D010936:Plant Extracts; D013662:Tea