Production of oligoglucuronans using a monolithic enzymatic microreactor.
A glucuronan lyase (EC 4.2.2.14) was immobilized on a monolithic Convective Interaction Media (CIM) disk. The immobilization yield was equal to 29% of the initial activity and 35% of the initial protein amount. Degradations of three glucuronans with various O-acetylation degrees were investigated and compared with degradations using free enzyme. The immobilized glucuronan lyase was inhibited by the O-acetylation degree like the free enzyme. (1)H NMR analyses were used to study the O-acetylation degree of oligoglucuronans and demonstrated that the average degrees of polymerization were inclusive between 4 and 13 after 24h of degradation. This first immobilization of a glucuronan lyase constitutes a new tool to produce oligoglucuronans.
Tavernier
M L
ML
Université de Picardie Jules Verne, Avenue des facultés, Le Bailly, F-80025 Amiens Cedex 1, France.
Petit
E
E
Delattre
C
C
Courtois
B
B
Courtois
J
J
Strancar
A
A
Michaud
P
P
D000107:Acetylation; D019149:Bioreactors; D001709:Biotechnology / Q000379:methods; D002621:Chemistry / Q000379:methods*; D004800:Enzymes, Immobilized / Q000737:chemistry; D005965:Glucuronates / Q000737:chemistry / Q000302:isolation & purification*; D006863:Hydrogen-Ion Concentration; D009682:Magnetic Resonance Spectroscopy; D009844:Oligosaccharides / Q000737:chemistry / Q000302:isolation & purification*; D011108:Polymers / Q000737:chemistry; D011133:Polysaccharide-Lyases / Q000737:chemistry*; D013696:Temperature; D013997:Time Factors; D014242:Trichoderma / Q000378:metabolism*
D004800:Enzymes, Immobilized; D005965:Glucuronates; D009844:Oligosaccharides; D011108:Polymers; D011133:Polysaccharide-Lyases; C109209:glucuronan lyase