title: Optimized preparation and scanning electrochemical microscopy analysis in feedback mode of glucose oxidase layers grafted onto conducting carbon surfaces.
abstract: An optimized immobilization procedure based on the electroreduction of aryldiazonium salt followed by covalent attachment of a cross-linked hydrogel was used to graft glucose oxidase on a carbon surface. Scanning electrochemical microscopy (SECM) and cyclic voltammetry were used to follow the construction steps of the modified electrode. By adjusting the compactness of the layer through the electrografting reaction, the penetration of the mediator through the layer can be controlled to allow the monitoring of the enzymatic activity by both cyclic voltammetry and SECM in feedback mode. The enzymatic activity of the film is finally characterized by SECM.
authors.affiliation: Sciences Chimiques de Rennes, Equipe MaCSE, Cedex, France.
authors.forename: Marie
authors.initials: M
authors.lastname: Pellissier
authors.forename: Dodzi
authors.initials: D
authors.lastname: Zigah
authors.forename: Frédéric
authors.initials: F
authors.lastname: Barrière
authors.forename: Philippe
authors.initials: P
authors.lastname: Hapiot
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D002244:Carbon / Q000737:chemistry*; D003432:Cross-Linking Reagents / Q000737:chemistry; D004563:Electrochemistry / Q000295:instrumentation*; D005949:Glucose Oxidase / Q000737:chemistry* / Q000378:metabolism*; D020527:Microscopy, Scanning Probe / Q000295:instrumentation / Q000379:methods*; D015394:Molecular Structure; D013499:Surface Properties
chemical_list: D003432:Cross-Linking Reagents; D002244:Carbon; D005949:Glucose Oxidase