Synthetic Studies in Phytochrome Chemistry.
An account is given of the author's several approaches to the synthesis of the parent chromophore of phytochrome (1), a protein-bound linear tetrapyrrole derivative that controls photomorphogenesis in higher plants. These studies culminated in enantioselective syntheses of both 2R- and 2S-phytochromobilin (4), as well as several (13)C-labeled derivatives designed to probe the site of Z,E-isomerization during photoexcitation. When reacted in vitro, synthetic 2R-4 and recombinant-derived phytochrome apoprotein N-C produced a protein-bound chromophore with identical difference spectra to naturally occurring 1.
Jacobi
Peter A
PA
Department of Chemistry, Dartmouth College, Hanover, New Hampshire 03755, USA.
Adel Odeh
Imad M
IM
Buddhu
Subhas C
SC
Cai
Guolin
G
Rajeswari
Sundaramoorthi
S
Fry
Douglas
D
Zheng
Wanjun
W
Desimone
Robert W
RW
Guo
Jiasheng
J
Coutts
Lisa D
LD
Hauck
Sheila I
SI
Leung
Sam H
SH
Ghosh
Indranath
I
Pippin
Douglas
D