The genome of a baculovirus infecting Tipula paludosa (Meig) (diptera): a high molecular weight closed circular DNA of zero superhelix density.
A characterization of the genome of a baculovirus infecting the diptera, Tipula paludosa Meig, is presented here. It appears to be a double-stranded closed circular DNA. The supercoiled structure has been visualized by electron microscopy. The relaxed molecules have a contour length of 51.2 +/- 1.5 mum which corresponds to a molecular weight of 92 x 10(6) daltons, by taking the value of 6.4 x 10(6) daltons for PM2 DNA used as an internal standard for the spreading. T. paludosa baculovirus DNA has a low G + C content of 27%, as estimated from its buoyant density in CsCl, uv spectrum, and melting temperature. Its sedimentation coefficient in alkaline solution was 294 S, as determined by analytical sedimentation.
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Institut de Recherches Scientifiques sur le Cancer, B.P. No. 8, 9 800 Villejuif, France.
Guelpa
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