[Impact of benzo [a] pyrene the expression of mitochondrion-encoded genes in the earthworm Eisenia fetida].
The earthworm Eisenia fetida's benzo [a] pyrene (BaP) exposure experiments were carried out in artificial soil according to ISO 11268-1:1993. And then the upregulated and downregulated subtractive cDNA libraries were constructed by Clontech PCR-Select cDNA Subtration Kit. From the BaP exposure upregulated subtractive cDNA library, several cDNA segments matched mitochondrion-encoded genes were found, including cytochrome c oxidase subunit I (CO I), subunit II (CO II), subunit Ill (CO III), NADH dehydrogenase subunit 1 (NDH1), and ATP synthase subunit 6. The result indicated BaP and the subsequent oxidative stress disturbed the expression of mitochondrion-encoded genes, and this was potential biomarker for oxidative stress following xenobiotic exposure.
Zheng
Sen-lin
SL
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China. zhengsenlin@yahoo.com.cn
Song
Yu-fang
YF
Qiu
Xiao-yan
XY
Sun
Tie-heng
TH
Zhang
Wei
W
Ackland
M L
ML
D000595:Amino Acid Sequence; D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D004272:DNA, Mitochondrial / Q000737:chemistry / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D050259:Genes, Mitochondrial / Q000187:drug effects* / Q000235:genetics; D025261:Mitochondrial Proton-Translocating ATPases / Q000235:genetics; D008969:Molecular Sequence Data; D009245:NADH Dehydrogenase / Q000235:genetics; D009835:Oligochaeta / Q000187:drug effects* / Q000235:genetics; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D012989:Soil Pollutants / Q000633:toxicity*
D004272:DNA, Mitochondrial; D012989:Soil Pollutants; D001564:Benzo(a)pyrene; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV; C412204:ATP synthase subunit 6; D025261:Mitochondrial Proton-Translocating ATPases