title: A highly basic cyanogen bromide peptide from sowbane mosaic virus protein.
abstract: The protein of sowbane mosaic virus was cleaved with cyanogen bromide (CNBr) and a highly basic peptide, sCB-1, was isolated by Sephadex and ion-exchange chromatography. The amino acid composition and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that sCB-1 had 49 amino acid residues. Eighteen of these residues are basic, fifteen are lysine, and three are arginine. sCB-1 moved faster in polyacrylamide gel electrophoresis at pH 2.2 than a similar sized CNBr peptide from the bean strain of southern bean mosaic virus with twelve basic residues. The highly basic nature of sCB-1 suggests that this portion of the SoMV protein sequence is involved in protein-RNA binding in the virus particles.
authors.affiliation: Research Station, Agriculture Canada, 6660 N. W. Marine Drive, Vancouver, B. C. V6T 1X2 Canada.
authors.forename: J H
authors.initials: JH
authors.lastname: Tremaine
authors.forename: W P
authors.initials: WP
authors.lastname: Ronald
authors.forename: E M
authors.initials: EM
authors.lastname: Kelly