Short-term response of soil bacteria to carbon enrichment in different soil microsites.
The response of bacteria in bulk soil and earthworm casts to carbon enrichment was studied by an RNA stable-isotope probing/terminal restriction fragment length polymorphism strategy with (13)C-labeled glucose and acetate. Both the soil microsite status and the carbon enrichment selected rapidly for different active bacterial communities, which resulted in different degradation kinetics. Our study clearly illustrates the biases that are generated by adding C substrates to detect metabolically active bacteria in soil.
Monard
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CNRS/Université de Rennes 1, UMR 6553 EcoBio, FR2116/FR90 Caren, Campus de Beaulieu, 263 Avenue du Général Leclerc, 35042 Rennes Cedex, France. cecilemonard@yahoo.fr
Binet
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F
Vandenkoornhuyse
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P
D000085:Acetates / Q000378:metabolism; D001419:Bacteria / Q000235:genetics / Q000378:metabolism*; D001673:Biodegradation, Environmental; D002247:Carbon Isotopes / Q000378:metabolism*; D017753:Ecosystem; D005947:Glucose / Q000378:metabolism; D012150:Polymorphism, Restriction Fragment Length; D025341:Principal Component Analysis; D012329:RNA, Bacterial / Q000235:genetics; D012988:Soil Microbiology
D000085:Acetates; D002247:Carbon Isotopes; D012329:RNA, Bacterial; D005947:Glucose