Detection and identification of wild yeasts in Champús, a fermented Colombian maize beverage.
The aim of this study was to identify and characterise the predominant yeasts in Champús, a traditional Colombian cereal-based beverage with a low alcoholic content. Samples of Champús from 20 production sites in the Cauca Valley region were analysed. A total of 235 yeast isolates were identified by conventional microbiological analyses and by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of ITS1-5.8S rDNA-ITS2. The dominant species were: Saccharomyces cerevisiae, Issatchenkia orientalis, Pichia fermentans, Pichia kluyveri var. kluyveri, Zygosaccharomyces fermentati, Torulospora delbruekii, Galactomyces geotrichum and Hanseniaspora spp. Model Champús systems were inoculated with single strains of some isolated sporogenus species and the aromatic profiles were analysed by SPME. Analysis of data showed that Champús strains produced high amounts of esters. The aromatic compounds produced by Saccharomyces and non-Saccharomyces yeasts from Champús can exert a relevant influence on the sensory characteristics of the fermented beverage. The Champús strains could thus represent an important source for new yeast biotypes with potential industrial applications.
Osorio-Cadavid
Esteban
E
Departamento de Biología, Universidad del Valle, Calle 13 No. 100-00, Cali, Colombia.
Chaves-López
Clemencia
C
Tofalo
Rosanna
R
Paparella
Antonello
A
Suzzi
Giovanna
G
D000434:Alcoholic Beverages / Q000382:microbiology*; D000704:Analysis of Variance; D002849:Chromatography, Gas; D004271:DNA, Fungal / Q000032:analysis; D021903:DNA, Ribosomal Spacer; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D005516:Food Microbiology; D006801:Humans; D016533:Mycological Typing Techniques; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012340:RNA, Ribosomal, 5.8S / Q000032:analysis; D017422:Sequence Analysis, DNA; D052617:Solid Phase Microextraction; D013045:Species Specificity; D014835:Volatilization; D015003:Yeasts / Q000145:classification* / Q000254:growth & development / Q000302:isolation & purification*; D003313:Zea mays
D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D012340:RNA, Ribosomal, 5.8S; D000431:Ethanol