title: Quercetin but not luteolin suppresses the induction of lethal shock upon infection of mice with Salmonella typhimurium.
abstract: Tumor necrosis factor-alpha (TNF-alpha) is important for the induction of systemic inflammatory responses that lead to lethal shock. Quercetin and luteolin, which differ by one hydroxyl group, are known to suppress the lipopolysaccharide-induced production of TNF-alpha in vitro. We show differing inhibitory effects of quercetin and luteolin on the induction of lethal shock in Salmonella typhimurium aroA-infected mice. In a time- and dose-dependent manner, quercetin reduced the plasma levels of TNF-alpha, lowered bacterial titers in livers, prevented liver damage and prolonged survival, while luteolin had little or no effect. Compared with luteolin, quercetin increased the infiltration of Gr-1(+)CD69(+) neutrophils into the peritoneal cavity and lowered heat shock protein 70 expression. Obviously, the additional hydroxyl group in quercetin is important for suppressing infection-induced lethal shock in mice.
authors.affiliation: Department of Biosciences, Graduate School of Science, Kitasato University, Kitasato, Sagamihara, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Sugiyama
authors.forename: Kiichiro
authors.initials: K
authors.lastname: Kawaguchi
authors.forename: Hideki
authors.initials: H
authors.lastname: Dobashi
authors.forename: Ryo
authors.initials: R
authors.lastname: Miyake
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kaneko
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Kumazawa
mesh_terms: D000818:Animals; D015169:Colony Count, Microbial; D004305:Dose-Response Relationship, Drug; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D007155:Immunologic Factors / Q000494:pharmacology*; D008099:Liver / Q000382:microbiology / Q000473:pathology; D047311:Luteolin / Q000494:pharmacology*; D051379:Mice; D015394:Molecular Structure; D009504:Neutrophils / Q000276:immunology; D010529:Peritoneal Cavity / Q000166:cytology; D011794:Quercetin / Q000494:pharmacology*; D012481:Salmonella Infections, Animal / Q000150:complications* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000302:isolation & purification*; D012769:Shock / Q000517:prevention & control*; D016019:Survival Analysis; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors* / Q000097:blood
chemical_list: D018840:HSP70 Heat-Shock Proteins; D007155:Immunologic Factors; D014409:Tumor Necrosis Factor-alpha; D011794:Quercetin; D047311:Luteolin