title: Development of a new assay for evaluation of l-3,4-dihydroxyphenylalanine cytotoxicity in catalase-mutant Escherichia coli.
abstract: The present study aimed to assess whether a newly constructed, catalase-deficient Escherichia coli strain that express mammalian catalase gene could be used to identify oxidative stress-generating chemicals. We tested l-3,4-dihydroxyphenylalanine (l-DOPA), a well-known agent that induces reactive oxygen species. We found that l-DOPA exposure reduced the survival of catalase-mutant E. coli in a dose-dependent manner, especially in the strains with lower catalase activities, implying the usefulness of these strains in assessment of oxidative chemicals.
authors.affiliation: Department of Public Health, Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Okayama 700-8558, Japan. dahong@md.okayama-u.ac.jp
authors.forename: Da-Hong
authors.initials: DH
authors.lastname: Wang
authors.forename: Masako
authors.initials: M
authors.lastname: Horita
authors.forename: Ken
authors.initials: K
authors.lastname: Tsutsui
authors.forename: Kuniaki
authors.initials: K
authors.lastname: Sano
authors.forename: Rani
authors.initials: R
authors.lastname: Sauriasari
authors.forename: Noriyoshi
authors.initials: N
authors.lastname: Masuoka
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takemura
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takigawa
authors.forename: Jiro
authors.initials: J
authors.lastname: Takaki
authors.forename: Keiki
authors.initials: K
authors.lastname: Ogino
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001681:Biological Assay / Q000379:methods*; D002374:Catalase / Q000235:genetics*; D004295:Dihydroxyphenylalanine / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000295:instrumentation* / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics*; D007980:Levodopa / Q000494:pharmacology; D009154:Mutation; D018384:Oxidative Stress; D017382:Reactive Oxygen Species
chemical_list: D001426:Bacterial Proteins; D017382:Reactive Oxygen Species; D007980:Levodopa; D004295:Dihydroxyphenylalanine; D002374:Catalase