title: Measurement of antioxidant ability of melatonin and serotonin by the DMPD and CUPRAC methods as trolox equivalent.
abstract: Melatonin (N-acetyl-5-methoxytryptamine) is the chief secretory product of the pineal gland and synthesized enzymatically from serotonin (5-hydroxytryptamine). These indoleamine derivatives play an important role in the prevention of oxidative damage. In the present study, DMPD radical scavenging and cupric ion (Cu(2+)) reducing ability of melatonin and serotonin as trolox equivalent antioxidant activity (TEAC) was investigated. Melatonin and serotonin demonstrated 73.5 and 127.4 microg/mL trolox equivalent DMPD( radical+) scavenging activity at the concentration of 100 microg/mL. Also, at the same concentration, melatonin and serotonin showed 14.41 and 116.09 microg/mL trolox equivalent cupric ion (Cu(2+)) reducing ability. These results showed that melatonin and serotonin had marked DMPD(radical+) radical scavenging and cupric ions (Cu(2+)) reducing ability. Especially, serotonin had higher DMPD radical scavenging and cupric ions (Cu(2+)) reducing activity than melatonin because of its phenolic group.
authors.affiliation: Department of Chemistry, Faculty of Science and Arts, Ataturk University, Erzurum, Turkey. igulcin@atauni.edu.tr
authors.forename: Ilhami
authors.initials: I
authors.lastname: Gulçin
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D002839:Chromans / Q000737:chemistry*; D003652:Decanoic Acids / Q000032:analysis* / Q000737:chemistry*; D005609:Free Radicals / Q000737:chemistry; D008550:Melatonin / Q000032:analysis* / Q000737:chemistry; D015394:Molecular Structure; D010881:Piperidones / Q000032:analysis* / Q000737:chemistry; D012701:Serotonin / Q000032:analysis* / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002839:Chromans; D003652:Decanoic Acids; D005609:Free Radicals; D010881:Piperidones; C491664:dimethylpiperidone; D012701:Serotonin; C031071:decanoic acid; D008550:Melatonin; C010643:6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid