title: [Establishing and identifying insulin resistance 3T3-L1 adipocytes cell model].
abstract: OBJECTIVE
  To establish insulin-resistant 3T3-L1 adipocytes cell model.
  
  
  METHODS
  3T3-L1 preadipocytes were cultured and induced to differentation. We established insulin resistant 3T3-L1 induced by dexamethasone and determined the change of glucose concentration in cell culture.
  
  
  RESULTS
  In high glucose DMEM culture media, the glusose concentration significantly decreased. Achieved the peak of insulin-resisitant at the 96th hour induced by dexamethasone, and at this time compared with the blank the mRNA expression of Resistin of the model step up about three times.
  
  
  CONCLUSIONS
  3T3-L1 adipocyte exposed to 1 micromol/L dexamethasone for 24 hours are able to induce a state of insulin resistance which can be maintained for 216 hours.
authors.affiliation: Life Science and Engineering Institute of Northwest University for Nationalities, Lanzhou 730030, China. gxnwww@126.com
authors.forename: Xiao-Nong
authors.initials: XN
authors.lastname: Guo
authors.forename: Ju-Tian
authors.initials: JT
authors.lastname: Yang
authors.forename: Feng
authors.initials: F
authors.lastname: Niu
authors.forename: Ru-Xue
authors.initials: RX
authors.lastname: Zhang
authors.forename: Zheng-Ping
authors.initials: ZP
authors.lastname: Jia
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D003470:Culture Media / Q000494:pharmacology; D003907:Dexamethasone / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005947:Glucose / Q000494:pharmacology*; D007333:Insulin Resistance; D051379:Mice; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D052243:Resistin / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D003470:Culture Media; D012333:RNA, Messenger; D052243:Resistin; D003907:Dexamethasone; D005947:Glucose