Inhibition of CD147 gene expression via RNA interference reduces tumor cell proliferation, activation, adhesion, and migration activity in the human Jurkat T-lymphoma cell line.
CD147, a leukocyte surface molecule over-expressed in T-lymphoma cells, is reportedly associated with lymphocyte activation and proteinase production via interactions with fibroblasts and plays a role in stromal invasion by lymphoma cells. To determine the role of CD147 in the progression of T-lymphoma, we performed siRNA interference-mediated knockdown of CD147 in a CD147-expressing Jurkat T-cell line. CD147 knockdown resulted in the decreased proliferation and migration of Jurkat cells and reduced the adhesion of Jurkat cells to extracelluar matrix fibronectin in vitro. CD147-siRNA inhibited the activation of Jurkat cells via down-regulation of CD25 expression. Our results indicate that CD147 is involved in T-lymphoma progression, a finding useful in efforts to develop targeted therapies to treat patients with T-lymphoma.
Chen
Xiang
X
Department of Dermatology, XiangYa Hospital, Central South University, Changsha, Hunan, China. chenxck@yahoo.com
Su
Juan
J
Chang
Jing
J
Kanekura
Takuro
T
Li
Ji
J
Kuang
Ye Hong
YH
Peng
Sheng
S
Yang
Fang
F
Lu
Hui
H
Zhang
Jiang Lin
JL
D000328:Adult; D051926:Basigin / Q000235:genetics / Q000378:metabolism*; D002448:Cell Adhesion / Q000235:genetics / Q000276:immunology; D002465:Cell Movement / Q000235:genetics / Q000276:immunology; D049109:Cell Proliferation; D015536:Down-Regulation; D005353:Fibronectins / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053645:Interleukin-2 Receptor alpha Subunit / Q000378:metabolism; D019169:Jurkat Cells; D008213:Lymphocyte Activation / Q000235:genetics; D016399:Lymphoma, T-Cell / Q000235:genetics / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D008875:Middle Aged; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D014162:Transfection
C497886:BSG protein, human; D005353:Fibronectins; D053645:Interleukin-2 Receptor alpha Subunit; D034741:RNA, Small Interfering; D051926:Basigin