A novel recombinant bacterial vaccine strain expressing dual viral antigens induces multiple immune responses to the Gag and gp120 proteins of HIV-1 in immunized mice.
Recombinant Salmonella enterica serovar Typhi can function as a live vector to deliver foreign antigens to the mammalian immune system and induce both mucosal and systemic immunity. In this study, we generated a recombinant Salmonella Typhi strain pilS-pilT-Gag+ (pVAX1-gp120) harboring the human immunodeficiency virus (HIV) gag gene integrated into the bacterial chromosome and gp120 gene carried by a plasmid. Mice inoculated with this recombinant bacterium through intranasal route produced high titers of IgG to gp120 in sera and IgA to gp120 in fecal washes. In addition, Gag-specific and gp120-specific cytotoxic T lymphocyte (CTL) responses were observed in sorted spleen lymphocytes of immunized mice. These results demonstrated that this recombinant Salmonella Typhi strain elicits multiple immune responses against both Gag and gp120 antigens of HIV, and thus would be a potential vaccine candidate to the prevention of HIV/AIDS.
Feng
Yong
Y
State Key Laboratory of Virology and College of Life Sciences, Wuhan University, Wychang, Wuhan 430072, Hubei, PR China.
Wang
Shiqun
S
Luo
Fenglin
F
Ruan
Ying
Y
Kang
Lei
L
Xiang
Xiaohui
X
Chao
Tao
T
Peng
Guiqing
G
Zhu
Chengliang
C
Mu
Yongxin
Y
Zhu
Ying
Y
Zhang
Xiaolian
X
Wu
Jianguo
J
D016915:AIDS Vaccines / Q000235:genetics / Q000276:immunology*; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D000956:Antigens, Viral / Q000235:genetics / Q000276:immunology*; D005260:Female; D015870:Gene Expression; D015699:HIV Envelope Protein gp120 / Q000235:genetics / Q000276:immunology*; D015658:HIV Infections / Q000276:immunology* / Q000821:virology; D015497:HIV-1 / Q000235:genetics / Q000276:immunology*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D051379:Mice; D008807:Mice, Inbred BALB C; D012485:Salmonella typhi / Q000235:genetics* / Q000378:metabolism; D013154:Spleen / Q000276:immunology / Q000821:virology; D013602:T-Lymphocytes, Cytotoxic / Q000276:immunology / Q000821:virology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D054301:gag Gene Products, Human Immunodeficiency Virus / Q000235:genetics / Q000276:immunology*
D016915:AIDS Vaccines; D000914:Antibodies, Viral; D000956:Antigens, Viral; D015699:HIV Envelope Protein gp120; D007074:Immunoglobulin G; D014614:Vaccines, Synthetic; D054301:gag Gene Products, Human Immunodeficiency Virus