title: Bone regeneration in cranioplasty and clinical complications in rabbits with alloxan-induced diabetes.
abstract: This research evaluated the bone repair process in surgical defects created on the parietal bones of diabetic rabbits using the guided bone regeneration technique to observe the effects of alloxan in the induction of diabetes mellitus. Twenty-four adult rabbits were divided into three study groups: control (C), diabetic (D) and diabetic associated to polytetrafluoroethylene (PTFE) membrane (D-PTFE). For diabetes induction the animals received one dose of monohydrated alloxan (90 mg/kg) by intravenous administration in the auricular or femoral vein. In group D-PTFE the membrane covered both the floor and the surface of the bone defect. In groups D and C, the bone defect was filled up with blood clot. The specimens were fixed in 10% formol and prepared for histomorphometric analysis. The results showed that the 90 mg/kg dose of monohydrate alloxan was sufficient to promote diabetes mellitus when administered in the auricular vein. Bone regeneration was slower in the diabetic group when compared with the control and diabetic-PTFE groups, but there was no significant statistical difference between the two experimental groups (D and D-PTFE). The oral and general clinical complications among the diabetics were weight loss, polyuria, polyphagia and severe chronic gingivitis.
authors.affiliation: Oral Biopathology Program, School of Dentistry of São José dos Campos, São Paulo State University, Brazil.
authors.forename: Evanice Menezes Marçal
authors.initials: EM
authors.lastname: Vieira
authors.forename: Camila Satie Ferreira
authors.initials: CS
authors.lastname: Ueno
authors.forename: Vivian Neves
authors.initials: VN
authors.lastname: Valva
authors.forename: Maria das Graças Vilela
authors.initials: Md
authors.lastname: Goulart
authors.forename: Terezinha de Oliveira
authors.initials: Tde O
authors.lastname: Nogueira
authors.forename: Mônica Fernandes
authors.initials: MF
authors.lastname: Gomes
mesh_terms: D000496:Alloxan; D000704:Analysis of Variance; D000818:Animals; D001672:Biocompatible Materials; D001861:Bone Regeneration / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000150:complications / Q000503:physiopathology* / Q000601:surgery; D004195:Disease Models, Animal; D010179:Pancreas / Q000187:drug effects; D010294:Parietal Bone / Q000601:surgery; D011138:Polytetrafluoroethylene; D011817:Rabbits; D011897:Random Allocation; D012886:Skull / Q000601:surgery*; D014945:Wound Healing / Q000502:physiology*
chemical_list: D001672:Biocompatible Materials; D000496:Alloxan; D011138:Polytetrafluoroethylene