Ribosome display selection of a metal-binding motif from an artificial peptide library.
A new ribosome display system was applied for the in vitro selection of a metal-binding motif from an artificial peptide library. The display system consisted of an mRNA-associating protein, a ribosome, and mRNA. The protein part of this display system was designed to provide a random peptide library and to stabilize the ribosome display. The random peptide library was newly designed to isolate stable metal-binding motifs. We employed the system for in vitro selection and found several new proteins and peptides that bind Co(II)-immobilized resin and Co(II)-complex, respectively. This newly developed system can be conveniently applied to the in vitro selection of peptide aptamers.
Wada
Akira
A
Nano Medical Engineering Laboratory, Discovery Research Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan. awada@riken.jp
Sawata
Shinya Y
SY
Ito
Yoshihiro
Y
D020816:Amino Acid Motifs / Q000235:genetics*; D003035:Cobalt / Q000737:chemistry*; D018388:Codon, Terminator; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D019020:Directed Molecular Evolution; D019151:Peptide Library; D011485:Protein Binding / Q000235:genetics; D054730:Protein Interaction Domains and Motifs / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012270:Ribosomes / Q000235:genetics* / Q000378:metabolism; D013269:Stochastic Processes
D018388:Codon, Terminator; D019151:Peptide Library; D012333:RNA, Messenger; D003035:Cobalt