Effects of cycloheximide on parthenogenetic development of pig oocytes activated by ultrasound treatment.
The present study was carried out to examine the parthenogenetic development of pig oocytes treated with different concentrations of cycloheximide for different durations following activation by ultrasound stimulation. When oocytes were treated with 10 microg/ml cycloheximide for different durations, the blastocyst formation rate of oocytes treated for 5 h was significantly (P<0.05) higher than those of oocytes treated for 0-2 h. The blastocyst formation rate of oocytes treated with 10 microg/ml cycloheximide for 5 h was significantly (P<0.05) higher than those of oocytes treated with 0-5 or 15-20 microg/ml cycloheximide for the same duration. When oocytes were treated with different concentrations of cycloheximide for 2 h, however, the blastocyst formation rate of oocytes treated with 40 microg/ml cycloheximide was significantly (P<0.05) higher than those of oocytes treated with 0-10 or 50 microg/ml cycloheximide. The blastocyst formation rate of oocytes treated with 10 microg/ml cycloheximide for 5 h was not significantly different from that of oocytes treated with 40 microg/ml cycloheximide for 2 h. These treatments did not affect the activation status of oocytes compared with controls that were not treated with cycloheximide. The results of the present study showed that cycloheximide improves the parthenogenetic development of pig oocytes activated by ultrasound stimulation.
Mori
Hironori
H
Laboratory of Animal Reproduction, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan.
Mizobe
Yamato
Y
Inoue
Shin
S
Uenohara
Akari
A
Takeda
Mitsuru
M
Yoshida
Mitsutoshi
M
Miyoshi
Kazuchika
K
D000818:Animals; D002478:Cells, Cultured; D003513:Cycloheximide / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D047108:Embryonic Development / Q000187:drug effects / Q000528:radiation effects; D005260:Female; D009865:Oocytes / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000528:radiation effects; D009866:Oogenesis / Q000187:drug effects / Q000528:radiation effects; D010312:Parthenogenesis / Q000187:drug effects* / Q000502:physiology / Q000528:radiation effects; D013552:Swine / Q000502:physiology*; D014465:Ultrasonics; D014463:Ultrasonography
D003513:Cycloheximide