Genetic typing of bovine viral diarrhoea virus: evidence of an increasing number of variants in Italy.
Bovine Viral Diarrhoea Virus (BVDV) is responsible worldwide for severe economic losses on cattle farms. BVDV is an RNA virus with a high genome variability having practical consequences on epidemiology, diagnosis and disease control. Genetic monitoring was suggested as the first step in BVDV control. Thirty-seven Bovine Viral Diarrhoea Viruses were identified in persistently infected cattle, mucosal disease-affected animals and in bulk milk, and were characterised genetically. The 5'UTR region was amplified and sequenced, and a phylogenetic analysis was carried out comparing all the Italian sequences of BVDV available from the Genbank database. An unusual number of persistent infected animals was evidenced on more than one farm. Phylogenetic analysis attributed all our viruses to BVDV type I and distinguished four different subgroups inside this genotype. Analysis of old and new viruses revealed the circulation of viruses classified in subgroups BVDV Ia and Ij never reported in Italy.
Ciulli
Sara
S
Department of Veterinary Public Health and Animal Pathology, Alma Mater Studiorum, University of Bologna, Ozzano Emilia, Italy. sara.ciulli@unibo.it
Galletti
Elena
E
Battilani
Mara
M
Scagliarini
Alessandra
A
Gentile
Arcangelo
A
Morganti
Luigi
L
Prosperi
Santino
S
D020121:5' Untranslated Regions; D000818:Animals; D001912:Bovine Virus Diarrhea-Mucosal Disease / Q000453:epidemiology / Q000821:virology*; D002417:Cattle; D003967:Diarrhea / Q000821:virology; D001908:Diarrhea Viruses, Bovine Viral / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D005243:Feces / Q000821:virology; D005838:Genotype; D007558:Italy / Q000453:epidemiology; D008892:Milk / Q000821:virology; D017720:Molecular Epidemiology; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D020121:5' Untranslated Regions