The leaf extract of Siberian Crabapple (Malus baccata (Linn.) Borkh) contains potential fatty acid synthase inhibitors.
The present work focused on the kinetics of the inhibitory effects of the leaf extract of Siberian Crabapple, named Shan jingzi in China, on chicken liver fatty acid synthase. The results showed that this extract had much stronger inhibitory ability on fatty acid synthase than that from green teas described in many previous reports. The inhibitory ability of this extract is closely related to the extracting solvent, and the time of extraction was also an important influencing factor. The inhibitory types of this extract on diffeerent substrates of chicken liver fatty acid synthase, acetyl-CoA, malonyl-CoA and NADPH, were found to be noncompetitive, uncompetitive and mixed, respectively. The studies here shed a new light on the exploration for inhibitors of fatty acid synthase.
Wei
Xiang
X
Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, People's Republic of China.
Zhao
Ran
R
Sun
Ying-Hui
YH
Cong
Jian-Ping
JP
Meng
Fan-Guo
FG
Zhou
Hai-Meng
HM
D000818:Animals; D002645:Chickens; D064429:Fatty Acid Synthases / Q000037:antagonists & inhibitors*; D007700:Kinetics; D027845:Malus / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D012997:Solvents
D010936:Plant Extracts; D012997:Solvents; D064429:Fatty Acid Synthases