title: Periodontal regeneration in experimentally-induced alveolar bone dehiscence by an improved porous biphasic calcium phosphate ceramic in beagle dogs.
abstract: Regeneration of lost periodontium is the focus of periodontal therapy. To achieve the effective regeneration, a number of bone graft substitute materials have been developed. This study aimed to investigate the histological response in alveolar bone dehiscences which were filled with an improved biphasic calcium phosphate (BCP) ceramic with more reasonable pore diameter, pore wall thickness and porosity. Twenty-four alveolar bone dehiscences were made surgically in twelve beagle dogs by reflecting mucoperiosteal flaps on the buccal aspect of bilateral lower second premolars and removing alveolar bone. The left dehiscences were treated with BCP ceramic and the contralaterals were cured with the open flap debridement (OFD) as controls. Three dogs were used at week 4, 12, and 24 respectively. Histological observations were processed through three-dimensional micro-computed tomographic imaging, fluorescence and light microscopy. The histological study indicated that the biphasic ceramic was biocompatible, and regeneration was achieved more effectively through the BCP treatment. There were also arrest of epithelial migration apically and formation of new bone and cementum, as well as proliferation of fibrous connective tissues that became attached to the newly formed cementum at week 24, while there was no significant periodontal regeneration in the OFD group only with epithelial tissue migrating into the dehiscence regions. Clinically speaking, though the surgical location formed a limitation to the application of the improved BCP on the periodontal regeneration, the actual result was positive. It proved that the BCP had biocompatibility and was able to act as a stable scaffold to induce periodontal regeneration effectively.
authors.affiliation: State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, People's Republic of China.
authors.forename: Han
authors.initials: H
authors.lastname: Shi
authors.forename: Jia
authors.initials: J
authors.lastname: Ma
authors.forename: Ning
authors.initials: N
authors.lastname: Zhao
authors.forename: Yangxi
authors.initials: Y
authors.lastname: Chen
authors.forename: Yunmao
authors.initials: Y
authors.lastname: Liao
mesh_terms: D016301:Alveolar Bone Loss / Q000473:pathology / Q000601:surgery / Q000628:therapy*; D000539:Alveolar Process / Q000473:pathology*; D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects / Q000502:physiology*; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D001842:Bone and Bones / Q000473:pathology / Q000601:surgery; D002130:Calcium Phosphates / Q000737:chemistry*; D002516:Ceramics / Q000737:chemistry* / Q000494:pharmacology; D004285:Dogs; D008297:Male; D010519:Periodontium / Q000473:pathology / Q000502:physiology / Q000601:surgery; D016062:Porosity; D012038:Regeneration / Q000502:physiology; D013997:Time Factors
chemical_list: D001672:Biocompatible Materials; D018786:Bone Substitutes; D002130:Calcium Phosphates; C020243:calcium phosphate