Adiponectin enhances in vitro development of swine embryos.
Recent studies have shown that factors from adipose tissue influence and regulate the reproductive system. Hormones such as leptin and resistin are now known to regulate several reproductive processes. Adiponectin is the most abundant protein secreted by adipose tissue, and its circulating concentration is inversely related to adiposity and body mass index. Little is known about the involvement of adiponectin in reproduction. In the present study, the effect of recombinant adiponectin on the meiotic maturation and early embryo development in vitro was investigated, using porcine oocytes. Adiponectin receptors, AdipoR1 and AdipoR2, were found to be expressed in porcine oocytes and cumulus cells of both small and large follicles. Both AdipoR1 and AdipoR2 were immunolocalized to cumulus-oocyte complexes (COCs), oocytes, and early developing embryos. When included in oocyte maturation medium for 46 h, adiponectin significantly decreased the frequency of meiotic immature oocytes derived from large follicles (3-6 mm) but not from small follicles (<3mm). From studies of oocytes matured in the presence of adiponectin and mitogen-activated protein kinase (MAPK) pathway inhibitors MEK1 (PD98059), MEK1/2 (U0126), and p38MAPK (SB203580) it was concluded that adiponectin enhances oocyte maturation thought the p38MAPK pathway. Finally, a superior rate of embryo development to the blastocyst stage was achieved by embryos cultured in the presence of adiponectin. These results indicate that adiponectin has a positive effect on the meiotic maturation and in vitro embryo development of porcine oocytes and suggests a physiological role for this adipokine in early development in mammals.
Chappaz
Eugénie
E
Department of Animal Science, McGill University, 21111 Lakeshore Road, Ste-Anne-de-Bellevue, Québec, Canada.
Albornoz
Marcelo S
MS
Campos
Danila
D
Che
Limei
L
Palin
Marie-France
MF
Murphy
Bruce D
BD
Bordignon
Vilceu
V
D052242:Adiponectin / Q000494:pharmacology*; D000818:Animals; D002070:Butadienes / Q000494:pharmacology; D047108:Embryonic Development / Q000187:drug effects / Q000502:physiology; D005260:Female; D005419:Flavonoids / Q000494:pharmacology; D007093:Imidazoles / Q000494:pharmacology; D007150:Immunohistochemistry / Q000662:veterinary; D020935:MAP Kinase Signaling System / Q000187:drug effects; D008297:Male; D008540:Meiosis / Q000187:drug effects / Q000502:physiology; D020928:Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism; D009570:Nitriles / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects* / Q000502:physiology*; D010312:Parthenogenesis / Q000187:drug effects; D011247:Pregnancy; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D012313:RNA / Q000737:chemistry / Q000235:genetics; D054419:Receptors, Adiponectin / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D013552:Swine / Q000196:embryology*
D052242:Adiponectin; D002070:Butadienes; D005419:Flavonoids; D007093:Imidazoles; D009570:Nitriles; D047428:Protein Kinase Inhibitors; D011725:Pyridines; D054419:Receptors, Adiponectin; C113580:U 0126; D012313:RNA; D020928:Mitogen-Activated Protein Kinases; C093642:SB 203580; C093973:2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one