title: INSIG2 gene rs7566605 polymorphism is associated with severe obesity in Japanese.
abstract: The single nucleotide polymorphism (SNP) rs7566605 in the upstream region of the insulin-induced gene 2 (INSIG2) is associated with the obesity phenotype in many Caucasian populations. In Japanese, this association with the obesity phenotype is not clear. To investigate the relationship between rs7566605 and obesity in Japanese, we genotyped rs7566605 from severely obese subjects [n = 908, body mass index (BMI) > or = 30 kg/m2] and normal-weight control subjects (n = 1495, BMI < 25 kg/m2). A case-control association analysis revealed that rs7566605 was significantly associated with obesity in Japanese. The P value in the minor allele recessive mode was 0.00020, and the odds ratio (OR) adjusted for gender and age was 1.61 [95% confidential interval (CI) = 1.24-2.09]. Obesity-associated phenotypes, which included the level of BMI, plasma glucose, hemoglobin A1c, total cholesterol, triglycerides, high-density lipoprotein (HDL) cholesterol, and blood pressure, were not associated with the rs7566605 genotype. Thus, rs7566605 in the upstream region of the INSIG2 gene was found to be associated with obesity, i.e., severe obesity, in Japanese.
authors.affiliation: Laboratory for Endocrinology and Metabolism, Center for Genomic Medicine, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan. kikuko@src.riken.jp.
authors.forename: Kikuko
authors.initials: K
authors.lastname: Hotta
authors.affiliation: Laboratory for Endocrinology and Metabolism, Center for Genomic Medicine, RIKEN, 1-7-22 Suehiro, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Michihiro
authors.initials: M
authors.lastname: Nakamura
authors.affiliation: Institute of Health and Sport Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Nakata
authors.affiliation: Institute of Health and Sport Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Matsuo
authors.affiliation: Medicine and Health Science Institute, Tokyo Medical University, Tokyo, Japan.
authors.forename: Seika
authors.initials: S
authors.lastname: Kamohara
authors.affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Kotani
authors.affiliation: Rinku General Medical Center, Osaka, Japan.
authors.forename: Ryoya
authors.initials: R
authors.lastname: Komatsu
authors.affiliation: Toyonaka Municipal Hospital, Osaka, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Itoh
authors.affiliation: Otemae Hospital, Osaka, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Mineo
authors.affiliation: Department of Medicine and Clinical Science, Okayama University Graduate School of Medicine and Dentistry, Okayama, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Wada
authors.affiliation: Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Masuzaki
authors.affiliation: Division of Gastroenterology, Yokohama City University Graduate School of Medicine, Kanagawa, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Yoneda
authors.affiliation: Division of Gastroenterology, Yokohama City University Graduate School of Medicine, Kanagawa, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Nakajima
authors.affiliation: Tokyo Postal Services Agency Hospital, Tokyo, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Miyazaki
authors.affiliation: Itami City Hospital, Hyogo, Japan.
authors.forename: Katsuto
authors.initials: K
authors.lastname: Tokunaga
authors.affiliation: Institute of Rheumatology, Tokyo Women's Medical University, Tokyo, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Kawamoto
authors.affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Funahashi
authors.affiliation: Department of Community Health and Gerontological Nursing, Faculty of Medicine, Oita University, Oita, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Hamaguchi
authors.affiliation: Department of Medicine, Division of Endocrinology and Metabolism, Kurume University, Fukuoka, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Yamada
authors.affiliation: First Department of Internal Medicine, Osaka Medical College, Osaka, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Hanafusa
authors.affiliation: Department of Medicine, Division of Endocrinology and Metabolism, Nippon Medical School, Tokyo, Japan.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Oikawa
authors.affiliation: Department of Anatomy, Biology and Medicine, Faculty of Medicine, Oita University, Oita, Japan.
authors.forename: Hironobu
authors.initials: H
authors.lastname: Yoshimatsu
authors.affiliation: Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Kyoto, Japan.
authors.forename: Kazuwa
authors.initials: K
authors.lastname: Nakao
authors.affiliation: Department of Anatomy, Biology and Medicine, Faculty of Medicine, Oita University, Oita, Japan.
authors.forename: Toshiie
authors.initials: T
authors.lastname: Sakata
authors.affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Matsuzawa
authors.affiliation: Institute of Health and Sport Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: Kiyoji
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: Institute of Rheumatology, Tokyo Women's Medical University, Tokyo, Japan.|Laboratory for Statistical Analysis, Center for Genomic Medicine, RIKEN, Tokyo, Japan.
authors.forename: Naoyuki
authors.initials: N
authors.lastname: Kamatani
authors.affiliation: Laboratory for Molecular Medicine, Human Genome Center, The Institute of Medical Science, University of Tokyo, Tokyo, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nakamura
mesh_terms: D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D008040:Genetic Linkage; D020022:Genetic Predisposition to Disease; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics*; D007564:Japan; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D009767:Obesity, Morbid / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide
chemical_list: C467727:INSIG2 protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008565:Membrane Proteins