Limited role of parental DNA in replication during infection by Bacillus subtilis phage SPO1.
When bacteria, growing in light medium, are infected with heavy phage, progeny DNA molecules band in the hybrid position only if they were synthesized using parental DNA as template. Thus, the rate of incorporation of radioactive precursors into hybrid DNA measures the use of parental template. This rate decreases with time during SPO1 infection, arguing against certain models for the control of SPOT replication.
Stewart
C R
CR
Department of Biology, Rice University, Houston, Texas 77001, USA.
Franck
M
M