title: Expression of 8-hydroxydeoxyguanosine in the gastric remnant after distal gastrectomy.
abstract: OBJECTIVE
  The correlation between remnant gastritis after distal gastrectomy for gastric cancer and expression of 8-hydroxydeoxyguanosine (8-OHdG) and inducible oxide synthase (iNOS) as a marker of oxidative DNA damage was investigated.
  
  
  METHODS
  Ninety-seven patients who had undergone curative distal gastrectomy for gastric cancer were studied. Reconstructive procedures included Billroth I, Billroth II, and Roux-Y reconstruction in 42, 27, and 28 patients, respectively. Histologic and immunohistochemical analyses were performed on biopsy specimens of the gastric mucosa obtained endoscopically within 2 weeks before and 12 weeks after surgery. The grades of remnant gastritis were evaluated according to the updated Sydney System. 8-OHdG and iNOS expression levels, detected immunohistochemically, were graded.
  
  
  RESULTS
  Neutrophil infiltration correlated with expression of 8-OHdG (p = 0.02). Expression of iNOS also correlated with 8-OHdG (p = 0.02). The ratio of postoperative to preoperative infiltration of neutrophils was less in patients who underwent Roux-Y reconstruction than in others (p = 0.04).
  
  
  CONCLUSIONS
  These results suggest that remnant gastritis possibly causes DNA damage. Excess production of reactive oxygen species correlates with carcinogenic DNA changes. Roux-Y reconstruction may reduce carcinogenesis in the gastric remnant.
authors.affiliation: Department of Gastroenterological Surgery, Osaka City University Graduate School of Medicine, Osaka, Japan. fuku@eva.hi-ho.ne.jp
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Fukuhara
authors.forename: Harushi
authors.initials: H
authors.lastname: Osugi
authors.forename: Masashi
authors.initials: M
authors.lastname: Takemura
authors.forename: Shigeru
authors.initials: S
authors.lastname: Lee
authors.forename: Keiichirou
authors.initials: K
authors.lastname: Morimura
authors.forename: Takayuki
authors.initials: T
authors.lastname: Nishikawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Iwasaki
authors.forename: Shoji
authors.initials: S
authors.lastname: Fukushima
authors.forename: Shigefumi
authors.initials: S
authors.lastname: Suehiro
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D003849:Deoxyguanosine / Q000031:analogs & derivatives* / Q000096:biosynthesis; D005743:Gastrectomy; D005753:Gastric Mucosa / Q000378:metabolism; D018530:Gastric Stump; D006801:Humans; D052247:Nitric Oxide Synthase Type II / Q000096:biosynthesis; D013274:Stomach Neoplasms / Q000601:surgery
chemical_list: D000080242:8-Hydroxy-2'-Deoxyguanosine; D052247:Nitric Oxide Synthase Type II; D003849:Deoxyguanosine