Hepatocyte growth factor suppresses production of reactive oxygen species and release of eosinophil-derived neurotoxin from human eosinophils.
BACKGROUND
Reactive oxygen species (ROS) and eosinophilic granule proteins such as eosinophil-derived neurotoxin (EDN) are known to damage bronchial tissue and cause airway hyperresponsiveness (AHR) in asthma. Hepatocyte growth factor (HGF) regulates various biological activities and is known to be a multifunctional factor. In our previous study, we found that HGF suppressed allergic airway inflammation and AHR in a murine model of asthma. However, there have been few reports regarding the detailed mechanism of the anti-allergic effect of HGF in asthma. In this study, we investigated the potential of recombinant HGF to regulate the production of ROS and the release of EDN from human eosinophils.


METHODS
Eosinophils were isolated from subjects with mild eosinophilia by modified CD16-negative selection. We investigated the expression of CD69, an activation marker of eosinophils, on eosinophils, using flow cytometry. Further, ROS production from eosinophils was analyzed using luminol-dependent chemiluminescence, and EDN release was measured by ELISA.


RESULTS
Treatment with HGF suppressed interleukin-5-induced upregulation of CD69 expression, ROS production and EDN release from human eosinophils.


CONCLUSIONS
Taken together, these data suggest that in asthma, HGF attenuates allergic airway inflammation and AHR through at least the suppression of ROS production and EDN release from eosinophils.
Ito
Wataru
W
Department of Clinical and Laboratory Medicine, Akita University School of Medicine, Akita, Japan.
Takeda
Masahide
M
Fujita
Miyoshi
M
Kamada
Yumiko
Y
Kato
Hikari
H
Chiba
Takahito
T
Yamaguchi
Kazutoshi
K
Ueki
Shigeharu
S
Kayaba
Hiroyuki
H
Kanehiro
Arihiko
A
Chihara
Junichi
J
D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D040881:CD11a Antigen / Q000032:analysis; D002118:Calcium / Q000378:metabolism; D047208:Eosinophil-Derived Neurotoxin / Q000378:metabolism*; D004804:Eosinophils / Q000187:drug effects* / Q000378:metabolism; D048049:Extracellular Signal-Regulated MAP Kinases / Q000502:physiology; D017228:Hepatocyte Growth Factor / Q000494:pharmacology*; D006801:Humans; D015848:Interleukin-5 / Q000494:pharmacology; D037181:Lectins, C-Type; D017382:Reactive Oxygen Species / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000502:physiology; D000099192:CD69 Antigens
D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D040881:CD11a Antigen; D002118:Calcium; D047208:Eosinophil-Derived Neurotoxin; D048049:Extracellular Signal-Regulated MAP Kinases; D017228:Hepatocyte Growth Factor; D015848:Interleukin-5; D037181:Lectins, C-Type; D017382:Reactive Oxygen Species; D048051:p38 Mitogen-Activated Protein Kinases; D000099192:CD69 Antigens