Similar sequences from the 5' end of cowpea mosaic virus RNAs.
Similar, large (N approximately 40) oligonucleotides are released from each of the two cowpea mosaic virus genomic RNAs by digestion with ribonuclease T1. Each large oligonucleotide was radiolabeled at the 3' end, and the nucleotide sequence was determined by base-specific partial degradation and analysis of the fragments. Specific deoxyoligonucleotides that are complementary to a portion of each large oligonucleotide were primers in reverse transcription reactions with intact virion RNA as template. Sequences to the 5' side of the large oligonucleotide sequences, and extending almost to the 5' end of each RNA, were read from analyses of transcripts that had been terminated by the incorporation of 2',3'-dideoxynucleoside-5'-phosphate residues. We report sequences of 87 and 79 nucleotide residues, respectively, from the two virus RNAs. The two sequences show many similarities and can be folded into a similar pattern to accommodate base pairing.
Najarian
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RC
Department of Biochemistry and Biophysics, University of California, Davis, California 95816, USA.
Bruening
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G