title: Low-molecular-weight RNAs associated with tobacco ringspot virus are satellites.
abstract: The low-molecular-weight RNA associated with an isolate of tobacco ringspot virus [TRSV(F)] lacks polyadenylic acid [poly(A)] sequences. Complementary DNA (cDNA) specific to this RNA was made with an oligo(dT) primer after tailing the RNA with poly(A). However, a significant amount of cDNA was made only when the RNA was treated with alkaline phosphatase prior to tailing. Hybridization of RNA in solution and by a Northern blot technique showed that there was no detectable nucleotide sequence homology between TRSV(F) RNA and two of the low-molecular-weight RNAs associated with this virus. These associated RNAs are therefore, satellites of TRSV(F). The relationship of these satellites to a previously reported satellite of TRSV is discussed.
authors.affiliation: Department of Biology, Shiraz University, Shiraz, Iran.
authors.forename: M A
authors.initials: MA
authors.lastname: Rezaian
authors.forename: A O
authors.initials: AO
authors.lastname: Jackson