Bioconversion of lactose/whey to fructose diphosphate with recombinant saccharomyces cerevisiae cells.
Genetically engineered Saccharomyces cerevisiae strains that express Escherichia coli beta-galactosidase gene are able to bioconvert lactose or whey into fructose-1,6-diphosphate (FDP). High FDP yields from whey were obtained with an appropriate ratio between cell concentration and inorganic phosphate. The biomass of transformed cells can be obtained from different carbon sources, according to the expression vector bearing the lacZ gene. We showed that whey can be used as the carbon source for S. cerevisiae growth and as the substrate for bioconversion to fructose diphosphate.
Compagno
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Dipartimento di Fisiologia e Biochimica Generali, sez. di Biochimica Comparata, Universitá di Milano, 20133 Milano, Italy.
Tura
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Ranzi
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Martegani
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