A simple technique to minimize heat damage to specimens during thermal polymerization of LR White in plastic and gelatin capsules.
London Resin (LR) White is a commonly used resin for embedding specimens to be used for immuno- and/or cytochemical studies. In some instances, due to either the properties of the specimen or the availability of various reagents and equipment, it becomes necessary and/or more convenient to polymerize LR White using heat rather than chemical accelerators or UV light. It is known, however, that heat can reduce or even eliminate the anti genicity of the tissue being embedded. It is therefore desirable to polymerize specimens at the lowest temperature possible and to remove the specimens from the oven as soon as polymerization is complete. We have developed a technique that provides a visual marker that allows the exothermic polymerization of LR White to be monitored, thus minimizing the amount of time a specimen must stay in the oven while excluding oxygen from capsules of polymerizing LR White.
Bowling
A J
AJ
Southern Weed Science Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Stoneville, MS 38776, USA.
Vaughn
K C
KC
D017360:Arabidopsis / Q000648:ultrastructure; D002214:Capsules / Q000737:chemistry*; D002736:Chloroplasts / Q000648:ultrastructure; D005780:Gelatin / Q000737:chemistry*; D003368:Gossypium / Q000648:ultrastructure; D006358:Hot Temperature; D007150:Immunohistochemistry / Q000379:methods*; D029069:Impatiens / Q000648:ultrastructure; D018515:Plant Leaves / Q000648:ultrastructure; D018517:Plant Roots / Q000648:ultrastructure; D010969:Plastics / Q000737:chemistry*; D011095:Polyethylenes / Q000737:chemistry; D011108:Polymers; D011126:Polypropylenes / Q000737:chemistry; D012116:Resins, Plant / Q000737:chemistry*; D016610:Tissue Embedding / Q000379:methods*
D002214:Capsules; C078194:London resin white; D010969:Plastics; D011095:Polyethylenes; D011108:Polymers; D011126:Polypropylenes; D012116:Resins, Plant; D005780:Gelatin