Effects of adiponectin on growth and differentiation of human keratinocytes--implication of impaired wound healing in diabetes.
Impaired wound healing is one of the most common complications associated with diabetes. Adiponectin is an abundant circulating adipocyte-derived cytokine that has beneficial effects on disorders accompanying diabetes. Herein we report that adiponectin has a regulatory effect on the growth and differentiation of HaCaT human keratinocyte cells. Furthermore, adiponectin regulated the expression of TGFbeta isoforms in keratinocytes in a dose-dependent manner, which implies that adiponectin modulates other types of cells related to wound repair via secretion of growth factors from keratinocytes. Moreover, TUNEL assay results revealed that adiponectin enhances apoptosis of keratinocytes. Taken together, the present results indicate that adiponectin has suppressive effects on the formation of hyperkeratosis commonly seen in patients with diabetic foot and its application may be beneficial for developing treatment for that disorder.
Kawai
Kenichiro
K
Department of Plastic and Reconstructive Surgery, Hyogo College of Medicine, 1-1 Mukogawacho, Nishinomiya, Hyogo 663-8501, Japan. k-kawai@hyo-med.ac.jp
Kageyama
Akiko
A
Tsumano
Tomoko
T
Nishimoto
Soh
S
Fukuda
Kenji
K
Yokoyama
Shigekazu
S
Oguma
Takashi
T
Fujita
Kazutoshi
K
Yoshimoto
Sakiya
S
Yanai
Ayako
A
Kakibuchi
Masao
M
D052242:Adiponectin / Q000494:pharmacology*; D017209:Apoptosis; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation; D003924:Diabetes Mellitus, Type 2 / Q000150:complications*; D017719:Diabetic Foot / Q000188:drug therapy; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000096:biosynthesis / Q000235:genetics; D014945:Wound Healing
D052242:Adiponectin; D016212:Transforming Growth Factor beta