A unique caleosin in oil bodies of lily pollen.
In view of the recent isolation of stable oil bodies as well as a unique oleosin from lily pollen, this study examined whether other minor proteins were present in this lipid-storage organelle. Immunological cross-recognition using antibodies against three minor oil-body proteins from sesame suggested that a putative caleosin was specifically detected in the oil-body fraction of pollen extract. A cDNA fragment encoding this putative pollen caleosin, obtained by PCR cloning, was confirmed by immunodetection and MALDI-MS analyses of the recombinant protein over-expressed in Escherichia coli and the native form. Caleosin in lily pollen oil bodies seemed to be a unique isoform distinct from that in lily seed oil bodies.
Jiang
Pei-Luen
PL
Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung 402, Taiwan.
Jauh
Guang-Yuh
GY
Wang
Co-Shing
CS
Tzen
Jason T C
JT
D000595:Amino Acid Sequence; D002135:Calcium-Binding Proteins / Q000737:chemistry / Q000235:genetics*; D003001:Cloning, Molecular; D004591:Electrophoresis, Polyacrylamide Gel; D017343:Genes, Plant; D027762:Lilium / Q000737:chemistry / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D010938:Plant Oils / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics*; D011058:Pollen / Q000737:chemistry / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D002135:Calcium-Binding Proteins; D010938:Plant Oils; D010940:Plant Proteins; D018749:RNA, Plant; C402836:caleosin protein, Sesamum indicum