Design and synthesis of regioisomerically pure unsymmetrical xanthene derivatives for staining live cells and their photochemical properties.
We have demonstrated the synthesis of regioisomerically pure unsymmetrical xanthene derivatives consisting of three units which can be independently modified to control their physical properties. The photochemical properties of the synthetic unsymmetrical xanthene derivatives were investigated in solution by UV-vis absorption and fluorescence measurements, and their cell imaging properties were examined by confocal laser-scanning microscopy.
Kamino
Shinichiro
S
Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan.
Ichikawa
Hayato
H
Wada
Shun-ichi
S
Horio
Yuka
Y
Usami
Yoshihide
Y
Yamaguchi
Takako
T
Koda
Toshiki
T
Harada
Aki
A
Shimanuki
Kazusa
K
Arimoto
Masao
M
Doi
Mitsunobu
M
Fujita
Yoshikazu
Y
D001577:Benzophenones / Q000138:chemical synthesis* / Q000737:chemistry; D002477:Cells / Q000187:drug effects; D004396:Coloring Agents / Q000138:chemical synthesis* / Q000737:chemistry; D005456:Fluorescent Dyes / Q000138:chemical synthesis* / Q000737:chemistry; D006801:Humans; D018613:Microscopy, Confocal; D008958:Models, Molecular; D015394:Molecular Structure; D010777:Photochemistry / Q000379:methods*; D013050:Spectrometry, Fluorescence; D013237:Stereoisomerism; D014407:Tumor Cells, Cultured; D014966:Xanthenes / Q000138:chemical synthesis* / Q000737:chemistry
D001577:Benzophenones; D004396:Coloring Agents; D005456:Fluorescent Dyes; D014966:Xanthenes