Conditional gene expression and targeting in neuroscience research.
Recently developed techniques for spatially and temporally controlled genetic manipulations based on regulated homologous recombination and/or transcription are extensively used in brain research. In addition to being important for testing the role of specific proteins in the central nervous system, these techniques allow analysis of brain functions at the neuronal circuit level. This overview discusses principles of conditional inactivation and expression of genes, and their specific applications to studies of the mammalian brain.
Morozov
Alexei
A
Unit on Behavioral Genetics, Laboratory of Molecular Pathophysiology, National Institute of Mental Health, Bethesda, Maryland, USA.
D000818:Animals; D001923:Brain Chemistry / Q000235:genetics*; D017353:Gene Deletion; D005786:Gene Expression Regulation / Q000235:genetics*; D018390:Gene Targeting / Q000379:methods*; D017930:Genes, Reporter / Q000235:genetics; D019426:Integrases / Q000235:genetics; D051379:Mice; D008967:Molecular Biology / Q000379:methods*; D016297:Mutagenesis, Site-Directed / Q000379:methods; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009488:Neurosciences / Q000379:methods*
D009419:Nerve Tissue Proteins; C045073:Cre recombinase; D019426:Integrases