Juvenile hormone induction of glutathione S-transferase activity in the larval fat body of the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae).
The effect of pyriproxyfen, a juvenile hormone analog (JHA), on the pupation of S. litura was examined. A topical application of 100 mug JHA/larva on the newly ecdysed (0-day) sixth instar larvae resulted in more than 80% pupation, while most of the 1- or 2-day-old larvae similarly treated developed into supernumerary larvae. Glutathione S-transferse (GST) activity in the fat body of 0-day-old sixth instar larvae was significantly induced within 12 h of JHA (100 microg/larva) treatment. In contrast, no such induction was found when 1- and 2-day-old sixth instar larvae were similarly treated. This induction phenomenon was also observed when 0-day-old sixth instar larvae were treated with the natural JH III. The suppressive effects of alpha-amanitin and cycloheximide suggest that JHA induction of GST activity in these cutworm larvae presumably occurred at the gene transcription level.
Wu
Ming-Cheng
MC
Department of Entomology, National Chung-Hsing University, Taiwan, ROC.
Lu
Kuang-Hui
KH
D053959:Alpha-Amanitin / Q000494:pharmacology; D000818:Animals; D003513:Cycloheximide / Q000494:pharmacology; D004790:Enzyme Induction / Q000187:drug effects; D005216:Fat Body / Q000187:drug effects* / Q000201:enzymology*; D005982:Glutathione Transferase / Q000096:biosynthesis / Q000378:metabolism*; D007605:Juvenile Hormones / Q000494:pharmacology*; D007814:Larva / Q000187:drug effects / Q000201:enzymology; D008675:Metamorphosis, Biological / Q000187:drug effects; D019384:Nucleic Acid Synthesis Inhibitors / Q000494:pharmacology; D011500:Protein Synthesis Inhibitors / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D018411:Spodoptera / Q000187:drug effects* / Q000201:enzymology*; D013997:Time Factors
D053959:Alpha-Amanitin; D007605:Juvenile Hormones; D019384:Nucleic Acid Synthesis Inhibitors; D011500:Protein Synthesis Inhibitors; D011725:Pyridines; C055613:pyriproxyfen; D003513:Cycloheximide; D005982:Glutathione Transferase