Mutations of the ALS gene endowing resistance to ALS-inhibiting herbicides in Lolium rigidum populations.
BACKGROUND
In the important grass weed Lolium rigidum (Gaud.), resistance to ALS-inhibiting herbicides has evolved widely in Australia. The authors have previously characterised the biochemical basis of ALS herbicide resistance in a number of L. rigidum biotypes and established that resistance can be due to a resistant ALS and/or enhanced herbicide metabolism. The purpose of this study was to identify specific resistance-endowing ALS gene mutation(s) in four resistant populations and to develop PCR-based molecular markers.


RESULTS
Six resistance-conferring ALS mutations were identified: Pro-197-Ala, Pro-197-Arg, Pro-197-Gln, Pro-197-Leu, Pro-197-Ser and Trp-574-Leu. All six mutations were found in one population (WLR1). Each Pro-197 mutation conferred resistance to the sulfonylurea (SU) herbicide sulfometuron, whereas the Trp-574-Leu mutation conferred resistance to both sulfometuron and the imidazolinone (IMS) herbicide imazapyr. A derived cleaved amplified polymorphic sequences (dCAPS) marker was developed for detecting resistance mutations at Pro-197. Furthermore, cleaved amplified polymorphic sequences (CAPS) markers were developed for detecting each of the six mutant resistant alleles. Using these markers, the authors revealed diverse ALS-resistant alleles and genotypes in these populations and related them directly to phenotypic resistance to ALS-inhibiting herbicides.


CONCLUSIONS
This study established the existence of a diversity of ALS gene mutations endowing resistance in L. rigidum populations: 1-6 different mutations were found within single populations. At field herbicide rates, resistance profiles were determined more by the specific mutation than by whether plants were homo- or heterozygous for the mutation.
Yu
Qin
Q
Western Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, Crawley, WA, Australia.
Han
Heping
H
Powles
Stephen B
SB
D000095:Acetolactate Synthase / Q000235:genetics*; D000483:Alleles; D019943:Amino Acid Substitution; D004252:DNA Mutational Analysis; D017343:Genes, Plant; D053209:Herbicide Resistance / Q000235:genetics*; D006579:Heterozygote; D006720:Homozygote; D008129:Lolium / Q000201:enzymology / Q000235:genetics*; D009154:Mutation; D021141:Nucleic Acid Amplification Techniques; D010641:Phenotype; D010940:Plant Proteins / Q000235:genetics*
D010940:Plant Proteins; D000095:Acetolactate Synthase