Turnip yellow mosaic virus RNA-replicase contains host and virus-encoded subunits.
The enzyme (RNA-replicase) involved in the synthesis of viral RNA has been purified from turnip yellow mosaic virus (TYMV)-infected chinese cabbage leaves. The RNA-replicaset contains two major subunits: one of apparent molecular weight 115,000 (115K) and the other of 45K. We have raised antisera against the purified TYMV-RNA-replicase and have demonstrated by immunoaffinity chromatography and immunoblotting that the 115K polypeptide is coded by the viral RNA but that the 45K protein is of host origin. Furthermore the TYMV RNA-replicase is clearly different from the RNA-dependent RNA polymerase that occurs in healthy as well as in infected plants.
Mouches
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C
Laboratoire de Biologie Cellulaire et Móleculaire, INRA et Université de Bordeaux II, Domaine de la Grande Ferrade, 38140 Pont de la Maye, France.
Candresse
T
T
Bove
J M
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