title: A simple and effective method to overcome the inhibition of Fe to PCR.
abstract: Fe inhibition to PCR can be overcome by adding greatly excessive EDTA first, and then the same amount Mg(2+) as EDTA to PCR mix. By this method impure DNA was amplified successfully. DNA samples containing metals whose complexation constant with EDTA was much higher than Mg(2+) can also be amplified.
authors.affiliation: Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Teng
authors.forename: Yuntao
authors.initials: Y
authors.lastname: Guan
authors.forename: Wanpeng
authors.initials: W
authors.lastname: Zhu
mesh_terms: D018441:Biofilms; D002614:Chelating Agents / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000737:chemistry* / Q000302:isolation & purification; D004492:Edetic Acid / Q000737:chemistry; D007501:Iron / Q000737:chemistry* / Q000494:pharmacology*; D008274:Magnesium / Q000737:chemistry; D016133:Polymerase Chain Reaction / Q000187:drug effects / Q000379:methods*
chemical_list: D002614:Chelating Agents; D004247:DNA; D004492:Edetic Acid; D007501:Iron; D008274:Magnesium