Transplantation of meniscus regenerated by tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells derived from rat bone marrow.
The purpose of this study was to assess transplantation of regenerated menisci using scaffolds from normal allogeneic menisci and bone-marrow-derived mesenchymal stromal cells (BM-MSCs) of rats. We reported that scaffolds derived from normal menisci seeded with BM-MSCs in vitro could form meniscal tissues within 4 weeks. Then, we hypothesized that our tissues could be more beneficial than allogeneic menisci regarding early maturation and chondroprotective effect. Bone marrow was aspirated from enhanced green fluorescent protein transgenic rats. BM-MSCs were isolated and seeded onto scaffolds which were prepared from Sprague-Dawley rat menisci. After 4 weeks in coculture, the tissues were transplanted to the defect of menisci. Repopulation of BM-MSCs and expression of extracellular matrices were observed in the transplanted tissues at 4 weeks after surgery. At 8 weeks, articular cartilage in the cell-free group was more damaged compared to that in the cell-seeded group or the meniscectomy group.
Yamasaki
Takuma
T
Department of Orthopaedic Surgery, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. tacyama@hiroshima-u.ac.jp
Deie
Masataka
M
Shinomiya
Rikuo
R
Yasunaga
Yuji
Y
Yanada
Shinobu
S
Ochi
Mitsuo
M
D000818:Animals; D030801:Animals, Genetically Modified; D001854:Bone Marrow Cells / Q000166:cytology* / Q000502:physiology*; D002478:Cells, Cultured; D005109:Extracellular Matrix / Q000502:physiology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D048091:Guided Tissue Regeneration; D008297:Male; D008592:Menisci, Tibial / Q000502:physiology / Q000637:transplantation*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D017154:Stromal Cells; D046509:Tissue Culture Techniques; D023822:Tissue Engineering; D054457:Tissue Scaffolds
D049452:Green Fluorescent Proteins