Assembling and imaging of his-tag green fluorescent protein on mica surfaces studied by atomic force microscopy and fluorescence microscopy.
The adsorption of his-tag green fluorescent protein (GFPH(6)) onto the mica surfaces has been studied by atomic force microscopy (AFM) and laser confocal fluorescence microscopy. By controlling the adsorption conditions, separated single GFPH(6) and GFPH(6) monolayer can be adsorbed and formed on mica surfaces. In present experiments, based on the AFM measurement, we found that the adsorbed GFPH(6) was bound on the mica surface with its beta-sheets. The formed GFPH(6) monolayer on mica surfaces was flat, uniform, and stable. Some applications of the formed monolayer have been demonstrated. The formed monolayer can be used as a substrate for DNA imaging and AFM mechanical lithography.
Liu
Zhiguo
Z
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China. zguoliu@yahoo.com.cn
Zu
Yuangang
Y
Fu
Yujie
Y
Zhang
Zhonghua
Z
Meng
Ronghua
R
D000327:Adsorption; D000538:Aluminum Silicates / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000378:metabolism* / Q000648:ultrastructure*; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D011485:Protein Binding
D000538:Aluminum Silicates; D049452:Green Fluorescent Proteins; C011934:mica