Anti-tumor effect of DNA-based vaccination and dSLIM immunomodulatory molecules in mice with Ph+ acute lymphoblastic leukaemia.
Since the prognosis of patients with Philadelphia chromosome positive acute lymphoblastic leukaemia (Ph+ ALL) still remains poor, new relapse prevention strategies are needed. We evaluated the pre-immunization of mice with DNA-based vaccines subsequently challenged by the syngeneic Ph+ ALL cell line BM185. Ballistic transfer of minimalistic immunogenically defined gene expression (MIDGE) vectors encoding a BCR-ABLp185 fusion specific peptide or GM-CSF were used for in vivo transfection. DNA-based double stem-loop immunomodulators (dSLIM) were used as immune adjuvant. We present survival and functional data that DNA-based vaccination with BCR-ABLp185 fusion specific sequences, GM-CSF and dSLIM leads to an anti-tumor effect in mice challenged with a lethal Ph+ ALL dose and this effect depends on leukaemia-specific sequences.
Köchling
Joachim
J
Department of Paediatrics, Universität zu Lübeck, Ratzeburger Allee 160, D-23538 Lübeck, Germany. koechling@paedia.ukl.mu-luebeck.de
Prada
Javier
J
Bahrami
Masoud
M
Stripecke
Renata
R
Seeger
Karl
K
Henze
Günter
G
Wittig
Burghardt
B
Schmidt
Manuel
M
D000818:Animals; D019496:Cancer Vaccines / Q000276:immunology*; D045744:Cell Line, Tumor; D005260:Female; D005822:Genetic Vectors; D007155:Immunologic Factors / Q000008:administration & dosage*; D051379:Mice; D008807:Mice, Inbred BALB C; D011119:Polynucleotides / Q000008:administration & dosage*; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000276:immunology* / Q000473:pathology / Q000517:prevention & control*; D055502:Secondary Prevention; D016019:Survival Analysis; D014162:Transfection / Q000379:methods; D019444:Vaccines, DNA / Q000276:immunology*
D019496:Cancer Vaccines; D007155:Immunologic Factors; D011119:Polynucleotides; D019444:Vaccines, DNA