Bioactive substance from Grifola frondosa (maitake) mushroom inhibits CCAAT enhancer binding protein beta and delta expression on C3H10T1/2 B2C1 adipocyte cells.
Bioactive compound substances from Grifola frondosa (maitake) inhibited adipocyte differentiation of B(2)C(1) preadipocytes. This compound is not related to MAP kinase (ERK1 and ERK2) cascades or beta-catenin, which inhibit the expression of Glut4, PPARgamma and C/EBPalpha. The compound reduced the expression time of C/EBPbeta and C/EBPdelta. These results suggest that the inhibitory action of these bioactive compounds on adipocyte differentiation is exhibited through preadipocytes. They cannot induce the expression of PPARgamma or C/EBPalpha because of the reduced expression time of C/EBPbeta and C/EBPdelta.
Minamino
Katsuhiko
K
Department of Food Science and Nutrition, School of Human Environmental Science, Mukogawa Women's University, Nishinomiya, Japan.
Yanaga
Yuri
Y
Ohtsuru
Masaru
M
D017667:Adipocytes / Q000378:metabolism*; D000818:Animals; D015153:Blotting, Western; D022782:CCAAT-Enhancer-Binding Protein-beta / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D051743:CCAAT-Enhancer-Binding Protein-delta / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D005786:Gene Expression Regulation / Q000235:genetics; D048691:Grifola / Q000737:chemistry; D051379:Mice; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D011817:Rabbits
D022782:CCAAT-Enhancer-Binding Protein-beta; D010936:Plant Extracts; D051743:CCAAT-Enhancer-Binding Protein-delta