Early induction of LDL receptor gene expression by genistein in DLD-1 colon cancer cell line.
The phytoestrogen genistein has been demonstrated to possess anti-tumor properties by mechanisms not yet clearly established. The present study was designed to investigate the effects of isoflavone genistein exposure at concentrations ranging from 0.01 microM to 50 microM on the LDL receptor and HMGCoA reductase gene expression in the estrogen receptor positive DLD-1 human colon cancer cell line. LDL receptor and HMGCoA reductase gene expressions were evaluated by reverse transcription followed by real-time PCR. Genistein induced an early increase of LDL receptor gene expression and later decreased HMGCoA reductase mRNA expression in DLD-1 cells. These findings provide direct evidence on the role of genistein in regulating LDL receptor and HMGCoA reductase gene expression in colon cancer.
Caruso
Maria Gabriella
MG
Laboratory of Biochemistry, National Institute for Digestive Diseases, Castellana Grotte (Bari), Italy. gabriella.caruso@libero.it
Messa
Caterina
C
Orlando
Antonella
A
D'Attoma
Benedetta
B
Notarnicola
Maria
M
D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy*; D005786:Gene Expression Regulation / Q000187:drug effects*; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D019833:Genistein / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D006903:Hydroxymethylglutaryl CoA Reductases / Q000235:genetics*; D048789:Phytoestrogens / Q000494:pharmacology* / Q000627:therapeutic use; D008517:Phytotherapy; D011973:Receptors, LDL / Q000235:genetics*
D000972:Antineoplastic Agents, Phytogenic; D048789:Phytoestrogens; D011973:Receptors, LDL; D019833:Genistein; D006903:Hydroxymethylglutaryl CoA Reductases