Metabolic and proteomic study of NS0 myeloma cell line following the adaptation to protein-free medium.
Proteomics and metabolomics technologies are potentially useful tool for the study of the very complex process of cell adaptation to protein-free medium. In this work, we used the iTRAQ technology to analyze different protein levels in adapted and non-adapted NS0 myeloma cell line. Several proteins with differential expression profile were characterized and quantified. Carbohydrate metabolism, protein synthesis and membrane transport were the principal pathways that change after the adaptation. Changes in lactate production rate with respect to glucose consumption rate were observed according to the changes observed by proteomic.
de la Luz-Hernández
K R
KR
Research and Development Direction, Center of Molecular Immunology, Havana, Cuba. katiar@cim.sld.cu
Rojas-del Calvo
L
L
Rabasa-Legón
Y
Y
Lage-Castellanos
A
A
Castillo-Vitlloch
A
A
Díaz
J
J
Gaskell
S
S
D000222:Adaptation, Physiological; D000818:Animals; D000911:Antibodies, Monoclonal; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D016895:Culture Media, Serum-Free; D066246:ErbB Receptors / Q000037:antagonists & inhibitors; D006801:Humans; D007328:Insulin; D051379:Mice; D009101:Multiple Myeloma / Q000378:metabolism*; D020543:Proteome; D040901:Proteomics
D000911:Antibodies, Monoclonal; D016895:Culture Media, Serum-Free; D007328:Insulin; D020543:Proteome; C512478:EGFR protein, human; D066246:ErbB Receptors