title: Crystalline degradation products of vancomycin as chiral stationary phase in microcolumn liquid chromatography.
abstract: The ability of crystalline degradation products (CDPs) of vancomycin as a chiral stationary phase was reported in a previous study for enantioselective separation of drugs, amino acids and agrochemical toxins by conventional LC column (250 x 4.6 mm). In this work, the potential of CDP of vancomycin for the enantiomeric separation in micro-LC (200 x 1 mm) has been studied. The obtained separation results are better than in our previous study with conventional LC columns. The enantiomers of D,L-phenylalanine, D,L-alanine, methyldopa, atropine and propranolol were used for this evaluation. Experiments have been carried out in a stainless steel tube that was packed with chiral silica particles of 3 and 12 microm diameters. Also, three different ratios of 3 and 12 microm silica particles were used for packing material of chiral columns and the effect on aspect ratio and resolving powers was compared.
authors.affiliation: Department of Chemistry, Faculty of Science, Shahid Beheshti University, Evin, Tehran, Iran. aghassempour@scientist.com
authors.forename: Alireza
authors.initials: A
authors.lastname: Ghassempour
authors.forename: Reza
authors.initials: R
authors.lastname: Alizadeh
authors.forename: Nahid Mashkori
authors.initials: NM
authors.lastname: Najafi
authors.forename: Ahmad
authors.initials: A
authors.lastname: Karami
authors.forename: Andreas
authors.initials: A
authors.lastname: Römpp
authors.forename: Bernhard
authors.initials: B
authors.lastname: Spengler
authors.forename: Hassan Y
authors.initials: HY
authors.lastname: Aboul-Enein
mesh_terms: D000409:Alanine / Q000737:chemistry / Q000302:isolation & purification; D001285:Atropine / Q000737:chemistry / Q000302:isolation & purification; D001711:Biotransformation; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002853:Chromatography, Liquid / Q000379:methods*; D003460:Crystallization; D008750:Methyldopa / Q000737:chemistry / Q000302:isolation & purification; D008832:Microchemistry / Q000379:methods; D010316:Particle Size; D010649:Phenylalanine / Q000737:chemistry / Q000302:isolation & purification; D011433:Propranolol / Q000737:chemistry / Q000302:isolation & purification; D012822:Silicon Dioxide; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013237:Stereoisomerism; D014640:Vancomycin / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D010649:Phenylalanine; D008750:Methyldopa; D014640:Vancomycin; D012822:Silicon Dioxide; D001285:Atropine; D011433:Propranolol; D000409:Alanine