[Simultaneous determination of sedecamycin and terdecamycin in livestock products by LC/MS].
A simple and rapid LC/MS method for simultaneous determination of sedecamyin (SCM) and terdecamycin (TDM) in livestock products has been developed. SCM and TDM were extracted with acetonitrile. The extract was washed with n-hexane and then evaporated to dryness. The residue was dissolved in methanol, and injected into the LC/MS. The mass spectrometer was operated in the positive electrospray ionization (ESI) mode. LC separation was performed on a high-pH-resistant C18 column with 10 mmol/L carbonic acid-ammonia buffer (pH 10.0)-acetonitrile as a mobile pahse. The recoveries from swine muscle and liver fortified at the levels of 0.01 and 0.05 microg/g were 77-88%, and those from poultry muscle and liver fortified at the levels of 0.01 and 0.3 microg/g were 51-93%. The quantitation limits of SCM and TDM were 0.008 microg/g and 0.005 microg/g, respectively.
Yatsukawa
Yoh-ichi
Y
Japan Food Research Laboratories, Tokyo, Japan.
Fujita
Kazuhiro
K
Nakamura
Munetomo
M
Watai
Masatoshi
M
Murayama
Mitsunori
M
Maitani
Tamio
T
D000818:Animals; D002645:Chickens; D002853:Chromatography, Liquid / Q000379:methods*; D018942:Macrolides / Q000032:analysis*; D013058:Mass Spectrometry / Q000379:methods*; D008460:Meat / Q000032:analysis*; D013552:Swine; D019155:Veterinary Drugs / Q000032:analysis*
D018942:Macrolides; D019155:Veterinary Drugs; C091722:terdecamycin; C054134:sedecamycin