A single amino acid of niapro of papaya ringspot virus determines host specificity for infection of papaya.
Most strains of Papaya ringspot virus (PRSV) belong to type W, causing severe loss on cucurbits worldwide, or type P, devastating papaya in tropical areas. While the host range of PRSV W is limited to plants of the families Chenopodiaceae and Cucuribitaceae, PRSV P, in addition, infects plants of the family Caricaceae (papaya family). To investigate one or more viral genetic determinants for papaya infection, recombinant viruses were constructed between PRSV P-YK and PRSV W-CI. Host reactions to recombinant viruses indicated that the viral genomic region covering the C-terminal region (142 residues) of NIaVPg, full NIaPro, and N-terminal region (18 residues) of NIb, is critical for papaya infection. Sequence analysis of this region revealed residue variations at position 176 of NIaVPg and positions 27 and 205 of NIaPro between type P and W viruses. Host reactions to the constructed mutants indicated that the amino acid Lys27 of NIaPro determines the host-specificity of PRSV for papaya infection. Predicted three-dimensional structures of NIaPros of parental viruses suggested that Lys27 does not affect the protease activity of NIaPro. Recovery of the infected plants from certain papaya-infecting mutants implied involvement of other viral factors for enhancing virulence and adaptation of PRSV on papaya.
Chen
Kuan-Chun
KC
Department of Plant Pathology, National Chung Hsing University, Taichung, Taiwan, ROC.
Chiang
Chu-Hui
CH
Raja
Joseph A J
JA
Liu
Fang-Lin
FL
Tai
Chun-Hsi
CH
Yeh
Shyi-Dong
SD
D000595:Amino Acid Sequence; D029441:Carica / Q000821:virology*; D010450:Endopeptidases / Q000235:genetics*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D017354:Point Mutation; D017800:Potyvirus / Q000201:enzymology / Q000235:genetics* / Q000472:pathogenicity*; D011487:Protein Conformation; D012367:RNA, Viral / Q000235:genetics; D020539:Sequence Analysis, Protein; D013045:Species Specificity; D014764:Viral Proteins / Q000235:genetics*
D012367:RNA, Viral; D014764:Viral Proteins; D010450:Endopeptidases; C059992:nuclear inclusion protein a, mosaic viruses