title: Novel apatite fiber scaffolds can promote three-dimensional proliferation of osteoblasts in rodent bone regeneration models.
abstract: We have successfully synthesized hydroxyapatite fibers via a homogenous precipitation method. Using these hydroxyapatite fibers, we have produced the apatite fiber scaffolds (AFS) with well-controlled pore sizes (porosity above 95%). The AFS is relatively simple to synthesize, and its porosity and pore size are controllable. The usefulness of AFS as a scaffold for bone regeneration was evaluated by (1) seeding and culturing cells in the AFS in vitro, (2) implanting the AFS seeded with cells inside the subcutaneous tissue of mice. The AFS had biocompatibility to support cell adhesion, proliferation, and differentiation. Ectopic bone formation could be formed in the AFS at 12 weeks after implantation into the subcutaneous tissue. Because of its high interpore connection, pore diameters, and porosity, it was believed that AFS was an effective scaffold that provided a three-dimensional cell culture environment. In both in vitro and in vivo environments, the more porous AFS was more advantageous in cell proliferation, cell adhesion, proliferating capacity, robust cell differentiation, ultimately inducing bone ingrowth inside the scaffolds.
authors.affiliation: Department of Orthopaedic Surgery, School of Medicine, Keio University, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Hikaru
authors.initials: H
authors.lastname: Morisue
authors.forename: Morio
authors.initials: M
authors.lastname: Matsumoto
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Chiba
authors.forename: Hideo
authors.initials: H
authors.lastname: Matsumoto
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Toyama
authors.forename: Mamoru
authors.initials: M
authors.lastname: Aizawa
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Kanzawa
authors.forename: Takahiro J
authors.initials: TJ
authors.lastname: Fujimi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Uchida
authors.forename: Isao
authors.initials: I
authors.lastname: Okada
mesh_terms: D000818:Animals; D001861:Bone Regeneration; D002448:Cell Adhesion; D002454:Cell Differentiation; D049109:Cell Proliferation; D017886:Durapatite / Q000138:chemical synthesis / Q000627:therapeutic use*; D051379:Mice; D010006:Osteoblasts / Q000166:cytology*; D016062:Porosity; D051381:Rats; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D017886:Durapatite