Recombination and selection can remove blaTEM alleles from bacterial populations.
We passaged cells expressing TEM-1 and TEM-12 from a single plasmid through either ampicillin or ceftazidime. We found that the combined effects of recombination and selection removed the bla(TEM-1) allele from the bacterial population when it was passaged through ceftazidime or the bla(TEM-12) allele when cultures were passaged through ampicillin.
Mroczkowska
Joanna E
JE
School of Natural Sciences, University of California, Merced, 5200 North Lake Road, Merced, California 95343, USA.
Barlow
Miriam
M
D000483:Alleles; D000667:Ampicillin / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002442:Ceftazidime / Q000494:pharmacology; D003470:Culture Media / Q000737:chemistry; D048168:Escherichia coli K12 / Q000187:drug effects* / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D011995:Recombination, Genetic; D012641:Selection, Genetic; D001618:beta-Lactamases / Q000235:genetics*
D000900:Anti-Bacterial Agents; D003470:Culture Media; D000667:Ampicillin; D002442:Ceftazidime; C088328:beta-lactamase TEM-12; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1