Rapid kinetic analysis of transcription elongation by Escherichia coli RNA polymerase.
Nucleotide incorporation during transcription by RNA polymerase is accompanied by pyrophosphate formation. Rapid release of pyrophosphate from the elongation complex at a rate consistent with productive transcription elongation occurs only in the presence of the correct next nucleotide for incorporation into the transcript.
Johnson
Ronald S
RS
Department of Biochemistry and Molecular Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27834, USA. johnsonro@ecu.edu
Strausbauch
Mark
M
Cooper
Ryan
R
Register
J Kristen
JK
D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D011756:Diphosphates / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D007700:Kinetics; D008969:Molecular Sequence Data; D009711:Nucleotides / Q000235:genetics; D013997:Time Factors; D014158:Transcription, Genetic
D011756:Diphosphates; D009711:Nucleotides; D012321:DNA-Directed RNA Polymerases