title: Critical role for TARPs in early development despite broad functional redundancy.
abstract: Transmembrane AMPA receptor regulatory proteins (TARPs), including gamma-2, gamma-3, gamma-4, and gamma-8, are auxiliary subunits for AMPA receptors. Based on studies in single knockout mice, it has been suggested that nearly all native AMPA receptors are associated with TARPs. To study the interplay between TARP family members and AMPA receptors in vivo, we generated mice lacking multiple TARPs. Triple knockout mice lacking gamma-3, gamma-4, and gamma-8 are viable and fertile, and synaptic AMPA receptor activity is reduced to a level comparable to that seen in gamma-8 single knockout mice. In contrast, triple knockout mice lacking gamma-2, gamma-3, and either gamma-4 or gamma-8 cannot survive ex utero. In particular, gamma-2, gamma-3, gamma-4 triple knockout mice are born apneic and paralyzed, despite normal AMPA receptor function in cortical and spinal neurons. We found that gamma-8 is expressed at low levels in early postnatal mice and regulates AMPA receptor levels at this developmental time period. Thus, the early expression of gamma-8 may be responsible for maintaining AMPA receptor functions in neonatal neurons. Together, our data indicate that TARPs, in particular gamma-2, are essential for early development, and that most neurons express multiple members of this functionally redundant protein family.
authors.affiliation: Department of Cellular and Molecular Pharmacology, University of California, Campus Box 2140, 600 16th Street, San Francisco, CA 94143-2140, USA.
authors.forename: Karen
authors.initials: K
authors.lastname: Menuz
authors.forename: Geoffrey A
authors.initials: GA
authors.lastname: Kerchner
authors.forename: Jessica L
authors.initials: JL
authors.lastname: O'Brien
authors.forename: Roger A
authors.initials: RA
authors.lastname: Nicoll
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000831:Animals, Newborn; D015220:Calcium Channels; D002478:Cells, Cultured; D004622:Embryo, Mammalian; D018683:Excitatory Amino Acid Agents / Q000494:pharmacology; D006624:Hippocampus / Q000166:cytology / Q000502:physiology; D066298:In Vitro Techniques; D008564:Membrane Potentials / Q000187:drug effects / Q000235:genetics; D008565:Membrane Proteins / Q000145:classification / Q000172:deficiency / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D018408:Patch-Clamp Techniques; D018091:Receptors, AMPA / Q000502:physiology; D013116:Spinal Cord / Q000166:cytology; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / Q000494:pharmacology
chemical_list: D015220:Calcium Channels; D018683:Excitatory Amino Acid Agents; D008565:Membrane Proteins; D018091:Receptors, AMPA; D018350:alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid