Amide bonds assemble pili on the surface of bacilli.
Pilin precursors are the building blocks of pili on the surface of Gram-positive bacteria; however, the assembly mechanisms of these adhesive fibers are unknown. Here, we describe the chemical bonds that assemble BcpA pilin subunits on the surface of Bacillus cereus. Sortase D cleaves BcpA precursor between the threonine (T) and the glycine (G) residues of its LPXTG sorting signal and catalyzes formation of an amide bond between threonine (T) of the sorting signal and lysine (K) in the YPKN motif of another BcpA subunit. Three CNA B domains of BcpA generate intramolecular amide bonds, and one of these contributes also to pilus formation. Conservation of catalysts and structural elements in pilin precursors in Gram-positive bacteria suggests a universal mechanism of fiber assembly.
Budzik
Jonathan M
JM
Department of Microbiology, University of Chicago, Chicago, IL 60637, USA.
Marraffini
Luciano A
LA
Souda
Puneet
P
Whitelegge
Julian P
JP
Faull
Kym F
KF
Schneewind
Olaf
O
D000577:Amides / Q000378:metabolism; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D019881:Aminoacyltransferases / Q000235:genetics / Q000378:metabolism; D001408:Bacillus anthracis / Q000235:genetics / Q000254:growth & development* / Q000502:physiology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000378:metabolism; D036781:Fimbriae Proteins / Q000737:chemistry / Q000235:genetics / Q000502:physiology; D010861:Fimbriae, Bacterial / Q000235:genetics / Q000502:physiology*; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016297:Mutagenesis, Site-Directed; D009876:Operon; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism
D000577:Amides; D001426:Bacterial Proteins; D011994:Recombinant Proteins; C509823:sortase D; D036781:Fimbriae Proteins; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases