title: In situ analysis of the bacterial community associated with the reindeer lichen Cladonia arbuscula reveals predominance of Alphaproteobacteria.
abstract: The diversity and spatial pattern of the bacterial community hosted by the shrub-like reindeer lichen Cladonia arbuscula were investigated by general DNA staining and FISH, coupled with confocal laser scanning microscopy (CLSM). Using an optimized protocol for FISH using cryosections of small lichen fragments, we found about 6 x 10(7) bacteria g(-1) of C. arbuscula. Approximately 86% of acridine orange-stained cells were also stained by the universal FISH probe EUB338. Using group-specific FISH probes, we detected a dominance of Alphaproteobacteria (more than 60% of all bacteria), while the abundance of Actinobacteria and Betaproteobacteria was much lower (<10%). Firmicutes were rarely detected, and no Gammaproteobacteria were present. Bacterial cells of different taxonomic groups are embedded in a biofilm-like, continuous layer on the internal surface of the C. arbuscula podetia, mainly occurring in small colonies of a few to a few hundred cells. The other parts of the lichen showed a lower bacterial colonization. alpha-proteobacterial 16S rRNA genes were amplified using total DNA extracts from C. arbuscula and separated by single-strand conformation polymorphism (SSCP). Sequencing of excised bands revealed the dominance of Acetobacteraceae.
authors.affiliation: Institute of Plant Sciences, Karl-Franzens-Universität Graz, Institute of Environmental Biotechnology, Graz University of Technology, Graz, Austria.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Cardinale
authors.forename: João
authors.initials: J
authors.lastname: Vieira de Castro
authors.forename: Henry
authors.initials: H
authors.lastname: Müller
authors.forename: Gabriele
authors.initials: G
authors.lastname: Berg
authors.forename: Martin
authors.initials: M
authors.lastname: Grube
mesh_terms: D020561:Alphaproteobacteria / Q000235:genetics*; D044822:Biodiversity; D004269:DNA, Bacterial / Q000235:genetics; D005798:Genes, Bacterial; D020459:Genes, rRNA; D025301:Hyphae / Q000166:cytology; D017404:In Situ Hybridization, Fluorescence; D008011:Lichens / Q000166:cytology / Q000382:microbiology*; D018613:Microscopy, Confocal; D018807:Polymorphism, Single-Stranded Conformational; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S