Prolactin-induced stimulation of H(3)-proline incorporation into the basement lamella of tadpole skin: light and electron microscope study.
Radioautographic studies revealed that prolactin markedly stimulates the incorporation of H(3)-proline into the basement lamella of the tail fin of frog tadpole. Radioactivity in fibroblasts reached a peak at 3 hours. Thereafter, the number of silver grains in fibroblasts was reduced with concomitant increase in the basement lamella. Fibroblasts of the prolactin animals also showed extensively developed rough endoplasmic reticulum and Golgi elements. Epidermis of control animals was labelled as in the prolactin animals, although the basement lamella was sparsely labelled. Fibroblasts, not the epidermis, seem to secrete collagen into the basement lamella.
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Department of Anatomy, New Jersey College of Medicine and Dentistry, Newark, New Jersey 07103, USA.