Messenger RNA expression of translationally controlled tumor protein (TCTP) in liver regeneration and cancer.
OBJECTIVE
Translationally controlled tumor protein (TCTP) was recently reported to be involved in tumor reversion. In order to understand TCTP mRNA transcript in proliferative tissue and malignant lesions, the mRNA expression of TCTP was determined in a rat model of liver regeneration and human liver cancer tissues. In addition, the potential role of TCTP on suppression of liver cancer was investigated.


METHODS
Liver regeneration model was established by a two-thirds partial hepatectomy (PH) in adult, male Sprague-Dawley rat. TCTP mRNA expression was determined by semiquantitative RT-PCR analysis. Antisense mRNA of TCTP was transfected into the SMMC-7721 liver cancer cell line. Biological assay of proliferation and cell cycle were analysed by MTT and flow cytometry, respectively.


RESULTS
After PH of rats, the level of TCTP mRNA transcript was upregulated slightly in liver tissue at 1 hour followed by a high expression from 3 to 12 hours, which then decreased dramatically at 24 hours before returning to original level during liver tissue proliferation. TCTP mRNA transcript increased significantly in liver cancer tissues when compared to non-cancerous adjacent tissues as control. The transfection with antisense oligodeoxynucleotides of TCTP led to decreased proliferation of SMMC-7721 cells resulting in cell cycle arrest and pro-apoptosis.


CONCLUSIONS
The results suggested that TCTP mRNA expression might be stage-specific in the proliferation of liver tissue but alter abnormally in cancer lesions. TCTP could be used as a potential target for suppression of liver tumorigenicity.
Zhu
Wu-Ling
WL
Department of Life Sciences and Technology, Xinxiang Medical University, Xinxiang, China. wulingzhu@xxmu.edu.cn
Cheng
Hai-Xia
HX
Han
Na
N
Liu
Dong-Liang
DL
Zhu
Wen-Xiao
WX
Fan
Bing-Lin
BL
Duan
Fang-Ling
FL
D000818:Animals; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D048708:Cell Growth Processes / Q000235:genetics; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008115:Liver Regeneration / Q000235:genetics*; D008297:Male; D016372:RNA, Antisense / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014162:Transfection; D000090324:Tumor Protein, Translationally-Controlled 1; D015854:Up-Regulation
D014408:Biomarkers, Tumor; D016372:RNA, Antisense; D012333:RNA, Messenger; C000715647:TPT1 protein, human; C000715649:Tpt1 protein, rat; D000090324:Tumor Protein, Translationally-Controlled 1