title: Molecular diversity and phylogeny of Hantaan virus in Guizhou, China: evidence for Guizhou as a radiation center of the present Hantaan virus.
abstract: To gain further insight into the molecular epidemiology of Hantaan virus (HTNV) in Guizhou, China, rodents were captured in this region in 2004 and 2005. In addition, serum samples were collected from four patients. Ten hantaviruses were isolated successfully in cell culture from four humans, two Apodemus agrarius, three Rattus norvegicus and one Rattus nitidus. The nucleotide sequences for their small (S), medium (M) and partial large (L) segments were determined. Phylogenetic analysis of the S and M segment sequences revealed that all of these isolates belong to the species HTNV, suggesting a spillover of HTNV from A. agrarius to Rattus rats. All available isolates from Guizhou were divided into four distinct groups either in the S segment tree or in the M segment tree. The clustering pattern of these isolates in the S segment tree was not in agreement with that in the M or L segment tree, showing that genetic reassortment between HTNV had occurred naturally. Analysis of the S segment sequences from available HTNV strains indicated that they formed three clades. The first clade, which comprised only viruses from Guizhou, was the outgroup of clades II and III. The viruses in the second clade were found in Guizhou and mainly in the far-east Asian region, including China. However, the viruses in the third clade were found in most areas of China, including Guizhou, in which haemorrhagic fever with renal syndrome (HFRS) is endemic. Our results reveal that the highest genetic diversity of HTNV is in a limited geographical region of Guizhou, and suggest that Guizhou might be a radiation centre of the present form of HTNV.
authors.affiliation: Beijing Friendship Hospital, Affiliate of Capital Medical University, Beijing, PR China.|Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou Province, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou Province, PR China.
authors.forename: Zhao-Xiao
authors.initials: ZX
authors.lastname: Wang
authors.affiliation: Guizhou Center for Disease Control and Prevention, Guiyang, Guizhou Province, PR China.
authors.forename: Ding-Ming
authors.initials: DM
authors.lastname: Wang
authors.affiliation: Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
authors.forename: Ming-Hui
authors.initials: MH
authors.lastname: Li
authors.affiliation: Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
authors.forename: Guo-Dong
authors.initials: GD
authors.lastname: Ren
authors.affiliation: Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
authors.forename: Zheng-Xiu
authors.initials: ZX
authors.lastname: Duan
authors.affiliation: Department of Pathology, University of Georgia, Athens, GA 30602, USA.
authors.forename: Zhen F
authors.initials: ZF
authors.lastname: Fu
authors.affiliation: Department of Virology, Haartman Institute, University of Helsinki, Finland.
authors.forename: Alexander
authors.initials: A
authors.lastname: Plyusnin
authors.affiliation: Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
authors.forename: Yong-Zhen
authors.initials: YZ
authors.lastname: Zhang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002681:China / Q000453:epidemiology; D002522:Chlorocebus aethiops; D014644:Genetic Variation; D018063:Hantaan virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D006480:Hemorrhagic Fever with Renal Syndrome / Q000453:epidemiology* / Q000662:veterinary / Q000821:virology; D006801:Humans; D008168:Lung / Q000821:virology; D017720:Molecular Epidemiology; D008969:Molecular Sequence Data; D051189:Murinae / Q000821:virology*; D010802:Phylogeny; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012376:Rodent Diseases / Q000453:epidemiology* / Q000821:virology; D017422:Sequence Analysis, DNA; D014709:Vero Cells