USAXS and SAXS from cancer-bearing breast tissue samples.
USAXS and SAXS patterns from cancer-bearing human breast tissue samples were recorded at beamline ID02 of the ESRF using a Bonse-Hart camera and a pinhole camera. The samples were classified as being ductal carcinoma, grade II, and ductal carcinoma in situ, partly invasive. The samples included areas of healthy collagen, invaded collagen, necrotic ducts with calcifications, and adipose tissue. The scattering patterns were analyzed in different ways to separate the scattering contribution and the direct beam from the observed rocking curve (RC) of the analyzer. It was found that USAXS from all tissues was weak, and the effects on the analyzer RC were observed only in the low-intensity tails of the patterns. The intrinsic RC was convolved with different model functions for the impulse response of the sample, and the best fit with experiment was obtained by the Pearson VII function. Significantly different distributions for the Pearson exponent m were obtained in benign and malignant regions of the samples. For a comparison with analyzer-based imaging (ABI) or diffraction enhanced imaging (DEI) a "long-slit" integration of the patterns was performed, and this emphasized the scattering contribution in the tails of the rocking curve.
Fernández
M
M
Department of Physical Sciences, University of Helsinki, POB 64, FIN-00014 University of Helsinki, Finland. manuel.fernandez@esrf.fr
Suhonen
H
H
Keyriläinen
J
J
Bravin
A
A
Fiedler
S
S
Karjalainen-Lindsberg
M-L
ML
Leidenius
M
M
von Smitten
K
K
Suortti
P
P
D000465:Algorithms; D005260:Female; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods; D066298:In Vitro Techniques; D008327:Mammography / Q000379:methods*; D011856:Radiographic Image Enhancement / Q000379:methods; D011857:Radiographic Image Interpretation, Computer-Assisted / Q000379:methods; D012031:Refractometry / Q000379:methods*; D015203:Reproducibility of Results; D053838:Scattering, Small Angle; D012680:Sensitivity and Specificity; D017356:Synchrotrons; D014056:Tomography, X-Ray / Q000379:methods*