Cytotoxicity of oxidised lipids in cultured colonal human intestinal cancer cells (caco-2 cells).
In this study, we investigated the extent of the cytotoxicity effect of oxidised lipids and whether tea catechins namely (-)epigallocatechin-3 gallate (EGCG) decreased lipid peroxidation in caco-2 cells. Cells treated with 0-100 microg/ml fish oil or methyl linoleate (ML) oxidised by UV irradiation for 24 h, 48 h and 72 h, indicated a substantial decrease in cell viability especially in samples treated with 100 microg/ml oxidised lipid. Addition of malondialdehyde (MDA) and hydroxynonenal (50 microM) also reduced cell viability. Using EGCG (50 microM) increased the viability of cells treated with 24 h oxidised mackerel oil (72% live and 28% dead) compared with 48 h oxidised mackerel oil (89% live and 11% dead) and 72 h oxidised mackerel oil (71% live and 29% dead) as monitored by the MTT assay. Apoptosis of caco-2 cells by oxidised fish oil and ML and protection by EGCG was confirmed using fluorescence microscopy and caspase-3 presence by Western blotting.
Alghazeer
Rabia
R
Faculty of Health and Medical Sciences, University of Surrey, Guildford, Surrey GU2 7XH, UK.
Gao
Hongliang
H
Howell
Nazlin K
NK
D000970:Antineoplastic Agents / Q000737:chemistry / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D006690:Bisbenzimidazole; D015153:Blotting, Western; D018938:Caco-2 Cells; D053148:Caspase 3 / Q000378:metabolism; D002392:Catechin / Q000494:pharmacology; D004396:Coloring Agents; D004273:DNA, Neoplasm / Q000096:biosynthesis / Q000235:genetics; D005395:Fish Oils / Q000737:chemistry / Q000528:radiation effects; D005455:Fluorescent Antibody Technique; D006801:Humans; D019787:Linoleic Acid / Q000737:chemistry / Q000494:pharmacology; D015227:Lipid Peroxidation / Q000187:drug effects; D008055:Lipids / Q000737:chemistry / Q000633:toxicity*; D010084:Oxidation-Reduction; D013662:Tea / Q000737:chemistry; D013778:Tetrazolium Salts; D013844:Thiazoles; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014466:Ultraviolet Rays
D000970:Antineoplastic Agents; D004396:Coloring Agents; D004273:DNA, Neoplasm; D005395:Fish Oils; D008055:Lipids; D013662:Tea; D013778:Tetrazolium Salts; D013844:Thiazoles; D017392:Thiobarbituric Acid Reactive Substances; D002392:Catechin; D019787:Linoleic Acid; D053148:Caspase 3; C022616:thiazolyl blue; D006690:Bisbenzimidazole