title: Fermentation and isolation of C10-deacetylase for the production of 10-deacetylbaccatin III from baccatin III.
abstract: 10-Deacetylabaccatin III (10 DAB), an important precursor for paclitaxel semisynthesis, is enhanced in yew extracts using C10-deacetylase and C13-deacylase enzymes.(4) C10-deacetylase is an intracellular enzyme produced by the fermentation of a soil microorganism, Nocardioides luteus (SC 13912). During the fermentation of Nocardioides luteus, the growth of cells reaches a maximum growth at 28 h. C10-deacetylase enzyme activity starts at 26 h and peaks at 38 h of the fermentation. The cells are recovered by centrifugation. The C10-deacetylase enzyme was purified from the Nocardioides luteus cells. The enzyme was purified 190-fold to near homogeneity. The purified enzyme appeared as a single band on 12.5% SDS-PAGE analysis with a molecular weight of 40,000 daltons. (c) 1995 John Wiley & Sons, Inc.
authors.affiliation: Department of Microbial Technology, Bristol-Myers Squibb Pharmaceutical Research Institute, One Squibb Drive, New Brunswick, New Jersey 08903.
authors.forename: V B
authors.initials: VB
authors.lastname: Nanduri
authors.forename: R L
authors.initials: RL
authors.lastname: Hanson
authors.forename: T L
authors.initials: TL
authors.lastname: Laporte
authors.forename: R Y
authors.initials: RY
authors.lastname: Ko
authors.forename: R N
authors.initials: RN
authors.lastname: Patel
authors.forename: L J
authors.initials: LJ
authors.lastname: Szarka