Improved assay for determining the total radical-scavenging capacity of antioxidants and foods.
Free radicals play a crucial role in the pathophysiology of human diseases such as cancer, atherosclerosis, and neurodegenerative diseases, and considerable attention has been focused on functional foods (or nutraceuticals) that are able to decrease the concentrations of free radicals and consequently protect against these diseases. The present study investigated an improved quantitative assay to measure antioxidant activity using the stable and fast-reacting chromogenic indicator [2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS). The ABTS-radical-scavenging activities of various antioxidants and apple extracts were measured in 96-well plates, and plots thereof were linearly interpolated, with the total radical-scavenging capacity quantified as the area under the curve. The first order of linear regression was obtained in a relationship between the absorbance reduction and various concentrations of the tested sample, and the total radical-scavenging capacity was expressed as the vitamin-C-equivalent antioxidant capacity. The advantages of this quantitative assay are that, first, it is fast, sensitive and confers little variation from experimental errors for single or mixed antioxidants; second, a large number of samples in a low quantity at a time can be run using 96-well plates.
Lee
Ki Won
KW
Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Kim
Young Jun
YJ
Kang
Nam Joo
NJ
Kim
Jong Hun
JH
Lee
Sang Jun
SJ
Kim
Dae-Ok
DO
Lee
Chang Yong
CY
Lee
Hyong Joo
HJ
D000975:Antioxidants / Q000032:analysis* / Q000494:pharmacology; D001205:Ascorbic Acid / Q000032:analysis / Q000494:pharmacology; D052160:Benzothiazoles; D002083:Butylated Hydroxyanisole / Q000032:analysis / Q000494:pharmacology; D002863:Chromogenic Compounds; D003124:Colorimetry / Q000379:methods; D005504:Food Analysis / Q000379:methods*; D016166:Free Radical Scavengers / Q000032:analysis* / Q000494:pharmacology; D006801:Humans; D044949:Kaempferols / Q000032:analysis / Q000494:pharmacology; D027845:Malus / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis / Q000494:pharmacology; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis / Q000494:pharmacology; D013451:Sulfonic Acids
D000975:Antioxidants; D052160:Benzothiazoles; D002863:Chromogenic Compounds; D016166:Free Radical Scavengers; D044949:Kaempferols; D010936:Plant Extracts; D013451:Sulfonic Acids; D002083:Butylated Hydroxyanisole; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C012526:quercitrin; C006552:kaempferol; D011794:Quercetin; D001205:Ascorbic Acid