Molecular characterization of the DYX1C1 gene and its application as a cancer biomarker.
OBJECTIVE
DYX1C1 has three alternatively spliced transcripts. Therefore, we expect that alternative transcripts of DYX1C1 are used as a biomarker to detect specific cancer.


METHODS
RT-PCR analysis is conducted in order to detect expression of the DYX1C1 gene and the PCR products were analyzed using the Image J program to compare the expression levels of each transcript.


RESULTS
We found one of the transcripts was directly associated with an HERV-H LTR element that could be translated into protein sequence. Four new alternative transcripts were identified by RT-PCR analysis with various human tissue samples including 10 normal and adjacent tumor tissue sets. Semi-quantitative RT-PCR analysis showed the transcriptional activity of V3 and V2 was higher in tumor than in normal tissue samples, especially in the colorectal tissue samples.


CONCLUSIONS
Our results indicated that alternatively spliced transcript variants of the DYX1C1 gene could be used as cancer biomarkers to detect colorectal cancer.
Kim
Yun-Ji
YJ
Division of Biological Sciences, College of Natural Sciences, Pusan National University, Busan, 609-735, Republic of Korea.
Huh
Jae-Won
JW
Kim
Dae-Soo
DS
Bae
Min-In
MI
Lee
Ja-Rang
JR
Ha
Hong-Seok
HS
Ahn
Kung
K
Kim
Tae-Oh
TO
Song
Geun-Am
GA
Kim
Heui-Soo
HS
D017398:Alternative Splicing; D014408:Biomarkers, Tumor / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000235:genetics*; D003598:Cytoskeletal Proteins; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D014644:Genetic Variation; D006801:Humans; D008297:Male; D009369:Neoplasms / Q000235:genetics*; D009419:Nerve Tissue Proteins / Q000235:genetics*; D009687:Nuclear Proteins / Q000235:genetics*; D011247:Pregnancy; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014158:Transcription, Genetic
D014408:Biomarkers, Tumor; D003598:Cytoskeletal Proteins; C478878:DNAAF4 protein, human; D009419:Nerve Tissue Proteins; D009687:Nuclear Proteins