Usefulness of strb1 and 16S rDNA-targeted PCR for detection of Streptomyces spp. in environmental samples.
In this study, we revealed rapid detection of streptomycin-producing Streptomyces spp. by extraction of total soil DNA from 14 soil samples using a modified lysis method followed by PCR amplification ofa genus-specific sequence in the Streptomyces' 16S rDNA gene. DNA band of the expected size (438 bp) was seen with all the samples. Additionally, specific amplification of the streptomycin-coding gene (strb1) directly from soil revealed the presence of a single DNA band of 940 bp. These results indicate that PCR-amplification of Streptomyces specific genes could be used for direct detection of streptomycin-producing Streptomyces species from soil.
Saadoun
Ismail
I
Department of Applied Biological Sciences, Jordan University of Science and Technology, Irbid, Jordan. isaadoun@just.edu.jo
Gharaibeh
Raad
R
D015373:Bacterial Typing Techniques; D004269:DNA, Bacterial / Q000032:analysis; D004275:DNA, Ribosomal / Q000032:analysis*; D004591:Electrophoresis, Polyacrylamide Gel; D007597:Jordan; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000032:analysis*; D012988:Soil Microbiology; D013302:Streptomyces / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D013307:Streptomycin / Q000032:analysis*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S; D013307:Streptomycin