Human interferon does not protect cowpea plant cell protoplasts against infection with alfalfa mosaic virus.
Orchansky et al. (P. Orchansky, M. Rubenstein, and I. Sela), Proc. Natl. Acad. Sci. USA 79, 2278-2280, 1982) reported that natural and recombinant human interferon (HuIFN), e.g., HuIFN-beta and -alpha subspecies gamma3, protects plant cells from infection with a plant virus (tobacco mosaic virus). For their study they used tobacco leaf discs as well as tobacco protoplasts. It is now reported that recombinant HuIFN-beta and HuIFN-alpha2 do not significantly decrease alfalfa mosaic virus increase in cowpea mesophyll protoplasts.
Huisman
M J
MJ
Department of Biochemistry, State University of Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands.
Broxterman
H J
HJ
Schellekens
H
H
van Vloten-Doting
L
L