Prediction of acute cellular renal allograft rejection by urinary metabolomics using MALDI-FTMS.
The present study investigated small molecule analysis of urinary samples as a noninvasive method to detect acute cellular renal allograft rejection. Matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) was used to analyze 15 urinary samples from transplant patients with different grades of biopsy showing improved clinical acute cellular rejection (ACR) and 24 urinary samples from 8 transplant patients without evidence of rejection. Seven small molecules demonstrated highly successful diagnostic performance (m/z): 278.1 (t = 3.398, p = 0.004), 293.0 (t = 2.169, p = 0.048), 294.1 (t = 2.154, p = 0.05), 382.2 (t = 2.961, p = 0.010), 383.3 (t = 2.270, p = 0.040), 402.2 (t = 2.994, p = 0.010), 424.0 (t = 2.644, p = 0.019). Kidney transplant patients with ACR could be distinguished from those without ACR using four individual small molecules with a specificity of 100%. In conclusion, the combination of MALDI-FTMS technology with a clear definition of patient groups can detect urine small molecule associated with ACR.
Wang
Ji-Na
JN
Department of Urology, Zhongshan Hospital Affiliated to Fudan University, Shanghai, China.
Zhou
Ying
Y
Zhu
Tong-Yu
TY
Wang
Xiangdong
X
Guo
Yin-Long
YL
D015415:Biomarkers / Q000652:urine; D019075:Electrophoresis, Capillary; D005583:Fourier Analysis; D006084:Graft Rejection / Q000175:diagnosis / Q000473:pathology / Q000652:urine*; D006801:Humans; D016030:Kidney Transplantation; D011237:Predictive Value of Tests; D020543:Proteome / Q000378:metabolism*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014184:Transplantation, Homologous
D015415:Biomarkers; D020543:Proteome