title: Production of the siderophore enterobactin: use of four different fermentation systems and identification of the compound by HPLC.
abstract: The article describes four different fermentation procedures for Escherichia coli AN311, a producer of enterobactin. A regular rotary shaker culture with a biphasic system consisting of an agar layer (as a reservoir for feeding processes) and a layer of liquid medium, 2.4 L and 10 L batch cultures, and a novel dialysis membrane fermentor were used. With the use of this latter fermentor type, the production of enterobactin could be increased by a factor of about 9.5, while growth increased by a factor of 12 compared to the other systems. For the rapid and reliable quantification of the concentration and purity of enterobactin an analytical and preparative high-performance liquid chromatography (HPLC) method was established. The degradation compounds of this siderophore were detected by diodearray and bioassays. A comparison of total catechol production as well as the distribution between enterobactin and its degradation compounds is given.
authors.affiliation: Biologisches institut, LB Mikrobiologie/Antibiotika, Universität Tübingen, auf der Morgenstelle 28, 7400 Tübingen, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Seiffert
authors.forename: K
authors.initials: K
authors.lastname: Goeke
authors.forename: H P
authors.initials: HP
authors.lastname: Fielder
authors.forename: H
authors.initials: H
authors.lastname: Zähner