title: The SNARE complex from yeast is partially unstructured on the membrane.
abstract: Molecular recognition between cognate SNAREs leads to the formation of a four-helix bundle, which facilitates vesicle docking and membrane fusion. For a SNARE system involved in trafficking in yeast, target membrane (t-) SNARE Sso1p and vesicle associated (v-) SNARE Snc2p contribute one SNARE motif each, whereas another t-SNARE (Sec9) donates two N-terminal and C-terminal SNARE motifs (SN1 and SN2) to the helical bundle. By use of EPR, it is found that SN2 has a tendency to be uncoiled, leaving a significant population of the SNARE complexes to be partially unstructured on the membrane. In sharp contrast, SN2 is fully engaged in the four-helix bundle when removed from the membrane, showing that the membrane is the main destabilizing factor. Helix-breaking proline mutations in SN2 did not affect the rate of docking but reduced the rate of lipid mixing significantly, indicating that SN2 plays an essential role in activating the transition from docking to fusion.
authors.affiliation: Department of Biochemistry, Biophysics, and Molecular Biology, Iowa State University, Ames, IA 50011, USA.
authors.forename: Zengliu
authors.initials: Z
authors.lastname: Su
authors.forename: Yuji
authors.initials: Y
authors.lastname: Ishitsuka
authors.forename: Taekjip
authors.initials: T
authors.lastname: Ha
authors.forename: Yeon-Kyun
authors.initials: YK
authors.lastname: Shin
mesh_terms: D020816:Amino Acid Motifs; D004578:Electron Spin Resonance Spectroscopy; D008561:Membrane Fusion; D008958:Models, Molecular; D009154:Mutation; D050767:Qc-SNARE Proteins / Q000737:chemistry* / Q000235:genetics; D050600:SNARE Proteins / Q000737:chemistry* / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry* / Q000235:genetics; D013113:Spin Labels; D053835:Unilamellar Liposomes / Q000737:chemistry
chemical_list: D050767:Qc-SNARE Proteins; C090117:SEC9 protein, S cerevisiae; D050600:SNARE Proteins; D029701:Saccharomyces cerevisiae Proteins; D013113:Spin Labels; D053835:Unilamellar Liposomes