Detection of the A189G mtDNA heteroplasmic mutation in relation to age in modern and ancient bones.
The aim of this study was to demonstrate the presence of the A189G age-related point mutation on DNA extracted from bone. For this, a peptide nucleic acid (PNA)/DNA sequencing method which can determine an age threshold for the appearance of the mutation was used. Initially, work was done in muscle tissue in order to evaluate the sensitivity of the technique and afterwards in bone samples from the same individuals. This method was also applied to ancient bones from six well-preserved skeletal remains. The mutation was invariably found in muscle, and at a rate of up to 20% in individuals over 60 years old. In modern bones, the mutation was detected in individuals aged 38 years old or more, at a rate of up to 1%, but its occurrence was not systematic (only four out of ten of the individuals over 50 years old carried the heteroplasmy). For ancient bones, the mutation was also found in the oldest individuals according to osteologic markers. The study of this type of age-related mutation and a more complete understanding of its manifestation has potentially useful applications. Combined with traditional age markers, it could improve identification accuracy in forensic cases or in anthropological studies of ancient populations.
Lacan
Marie
M
CNRS, FRE 2960, Laboratoire d'Anthropobiologie, 37 allées Jules Guesde, 31073, Toulouse, France.
Thèves
Catherine
C
Amory
Sylvain
S
Keyser
Christine
C
Crubézy
Eric
E
Salles
Jean-Pierre
JP
Ludes
Bertrand
B
Telmon
Norbert
N
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000375:Aging; D015139:Blotting, Southern; D004272:DNA, Mitochondrial / Q000235:genetics*; D005260:Female; D006801:Humans; D007223:Infant; D008297:Male; D008875:Middle Aged; D018482:Muscle, Skeletal / Q000473:pathology; D017354:Point Mutation; D016133:Polymerase Chain Reaction; D012272:Ribs / Q000473:pathology*; D017422:Sequence Analysis, DNA / Q000379:methods
D004272:DNA, Mitochondrial