Biodegradation process development using a bacterial cytochrome in vivo.
Pseudomonas putida PpG786 that contains the inducible enzyme system cytochrome P-450(cam) is considered for use as specialized biomass fore detoxification of hazardous hydrocarbons. The test substrate 1,2-dibromochloropropane (DBCP) is used to assess the organohalide degradation activity of P. Putida PpG786. Activity was found to be a strong function of intracellular heme content, variables which affect the culturing and processing of the cells, and oxygen tension in the degradation incubation medium. The lifetime for maintaing active biomass in chemostat washout operation, after including substrate was removed and then restarted, was also studied. These results indicate that initial activity of the P. Putida biomass is high enough, and decays slowly enough, so that industrial wastewater treatment at the operating conditions of a sequencing batch reactor (SBR) could remove hazardous compounds. (c) 1994 John Wiley & Sons, Inc.
Horowitz
J B
JB
Department of Chemical Engineering, University of California, Los Angles, California 90024.
Vilker
V L
VL