Circadian phenotypes of Drosophila fragile x mutants in alternative genetic backgrounds.
Drosophila FMR1 mutants are models of human fragile X syndrome. They show a loss of locomotor activity rhythm and severe degradation of eclosion timing. We analyzed the circadian behavior of FMR1 mutants (dfmr1B55) in two genetic backgrounds, yellow white (yw) and Canton S (CS). The arrhythmic phenotype of circadian locomotor activity in constant darkness (DD) did not significantly change in either genetic background. Surprisingly, eclosion timing was completely restored by backcrossing dfmr1B55 with yw or CS flies. Morphological analysis of the small ventrally located lateral neurons of FMR1 mutants revealed that the dorsal-projection area was significantly larger in arrhythmic than rhythmic flies. In addition, dfmr1B55 mutants in both genetic backgrounds had a significantly lower evening peak in the light-dark (LD) cycle. These results indicate that lack of FMR1 does not affect eclosion timing, but alters locomotor activity patterns in both LD and DD conditions by affecting the arborization of small ventrally located lateral neurons. Thus, the FMR1 gene may regulate the circadian-related locomotor activity of Drosophila.
Sekine
Tatsumori
T
National Institute of Agrobiological Science, Tsukuba, Ibaraki, Japan.
Yamaguchi
Terumi
T
Hamano
Kunikatsu
K
Siomi
Haruhiko
H
Saez
Lino
L
Ishida
Norio
N
Shimoda
Masami
M
D000818:Animals; D002940:Circadian Rhythm / Q000235:genetics* / Q000502:physiology; D003433:Crosses, Genetic; D017931:DNA Primers / Q000235:genetics; D004330:Drosophila / Q000235:genetics* / Q000502:physiology; D029721:Drosophila Proteins / Q000235:genetics*; D051860:Fragile X Messenger Ribonucleoprotein 1 / Q000235:genetics*; D007150:Immunohistochemistry; D008124:Locomotion / Q000235:genetics* / Q000502:physiology; D008156:Luciferases; D009154:Mutation / Q000235:genetics*; D009474:Neurons / Q000166:cytology; D010641:Phenotype; D017440:Photoperiod; D013997:Time Factors
D017931:DNA Primers; D029721:Drosophila Proteins; D051860:Fragile X Messenger Ribonucleoprotein 1; D008156:Luciferases; C467328:FMR1 protein, Drosophila