Resin comparison and fast automated stepwise conventional synthesis of human SDF-1alpha.
Human SDF-1alpha contains 68 amino acids and is a member of the chemokine family of peptides. This long peptide was synthesized stepwise using classical conditions in 101 h. The reaction times were then reduced to deprotection times of 2 x 2 min and coupling times of 2 x 2.5 min, resulting in a total synthesis time of 22 h. The effect of different resins, resin substitutions and deprotection reagents on the crude peptide purities was compared. A small portion of crude peptide was purified using an RP-HPLC column and the mass of the final product was confirmed with MALDI-TOF mass spectrometry.
Patel
Hirendra
H
Department of Chemistry, Protein Technologies Inc., 4675, South Coach Drive, Tucson, Arizona 85714, USA.
Chantell
Christina A
CA
Fuentes
German
G
Menakuru
Mahendra
M
Park
Jae H
JH
D002625:Chemistry, Organic / Q000379:methods*; D054377:Chemokine CXCL12 / Q000138:chemical synthesis* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D006801:Humans; D012117:Resins, Synthetic / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
D054377:Chemokine CXCL12; D012117:Resins, Synthetic