title: Influence of fluorinated and hydrogenated nanoparticles on the structure and fibrillogenesis of amyloid beta-peptide.
abstract: Peptide aggregation in amyloid fibrils is implicated in the pathogenesis of several diseases such as Alzheimer's disease. There is a strong correlation between amyloid fibril formation and a decrease in conformational stability of the native state. Amyloid-beta peptide (Abeta), the aggregating peptide in Alzheimer's disease, is natively unfolded. The deposits found in Alzheimer's disease are composed of Abeta fibrillar aggregates rich in beta-sheet structure. The influence of fluorinated complexes on the secondary structure and fibrillogenesis of Abeta peptide was studied by circular dichroism (CD) spectroscopy and transmission electron microscopy (TEM). CD spectra show that complexes of polyampholyte and fluorinated dodecanoic acid induce alpha-helix structure in Abeta, but their hydrogenated analogous lead to beta-sheet formation and aggregation. The fluorinated nanoparticles with highly negative zeta potential and hydrophobic fluorinated core have the fundamental characteristics to prevent Abeta fibrillogenesis.
authors.affiliation: LEPAE, Dept. of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal. sandra.rocha@fe.up.pt
authors.forename: Sandra
authors.initials: S
authors.lastname: Rocha
authors.forename: Andreas F
authors.initials: AF
authors.lastname: Thünemann
authors.forename: Maria do Carmo
authors.initials: Mdo C
authors.lastname: Pereira
authors.forename: Manuel
authors.initials: M
authors.lastname: Coelho
authors.forename: Helmuth
authors.initials: H
authors.lastname: Möhwald
authors.forename: Gerald
authors.initials: G
authors.lastname: Brezesinski
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry* / Q000378:metabolism; D016564:Amyloid beta-Protein Precursor / Q000737:chemistry; D005461:Fluorine / Q000737:chemistry*; D006859:Hydrogen / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D058255:Protease Nexins; D011956:Receptors, Cell Surface / Q000737:chemistry
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D058255:Protease Nexins; D011956:Receptors, Cell Surface; D005461:Fluorine; D006859:Hydrogen