title: Protein salting-out: phase equilibria in two-protein systems.
abstract: The phase behavior of two aqueous binary protein mixtures, lysozyme-chymotrypsin and lysozyme-ovalbumin, was determined in ammonium sulfate solutions. Protein concentrations were determined in both phases as a function of pH and ionic strength. For lysozyme-chymotrypsin mixtures, the observed phase behavior was similar to that for each individual protein; the presence of the second protein had little influence. The phase behavior of lysozyme-ovalbumin mixtures, however, was different from that of the respective single-protein systems. Lysozyme and ovalbumin are found together in egg whites; their association is both pH and ionic-strength dependent. The association of proteins is a key determinant of protein solubility in salt solutions.
authors.affiliation: Department of Chemical Engineering, University of California, Berkeley, California 94720-9989, USA.
authors.forename: C J
authors.initials: CJ
authors.lastname: Coen
authors.forename: J M
authors.initials: JM
authors.lastname: Prausnitz
authors.forename: H W
authors.initials: HW
authors.lastname: Blanch