Preparation of ethyl magnesium bromide for regiospecific analysis of triacylglycerols.
This paper presents a procedure for preparation of a Grignard reagent, ethyl magnesium bromide, used for partial deacylation of triacylglycerols (TAG) in their regiospecific analysis. Magnesium turnings were reacted with ethereal solution of bromoethane in a screw-capped test tube to synthesize 2 mL of 1 M ethyl magnesium bromide. Continuously stirred with a vortex mixer, the reaction smoothly proceeded at room temperature. Regiospecific analysis of 1,3-distearoyl-2-oleoylglycerol using this product showed that fatty acid compositions of the sn-1(3) and sn-2 positions were contaminated by less than 2 mol% of fatty acids migrated from isomeric positions. The analyses of lard and cod liver/mackerel oil TAG showed typical distribution patterns of 16:0, 22:5n-3 and 22:6n-3 in pig and fish depot TAG. These results confirmed the view that the freshly prepared reagent is usable for regiospecific analysis of TAG.
Ando
Yasuhiro
Y
Laboratory of Marine Bioresources Chemistry, Division of Marine Life Science, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Japan. ando@fish.hokudai.ac.jp <ando@fish.hokudai.ac.jp>
Tomita
Yuki
Y
Haba
Yusuke
Y
D000215:Acylation; D005227:Fatty Acids / Q000737:chemistry; D006842:Hydrocarbons, Brominated / Q000737:chemistry; D007536:Isomerism; D008956:Models, Chemical; D009942:Organometallic Compounds / Q000138:chemical synthesis*; D012996:Solutions / Q000737:chemistry; D013696:Temperature; D014280:Triglycerides / Q000032:analysis* / Q000737:chemistry
C117423:1,3-distearoyl-2-oleoylglycerol; D005227:Fatty Acids; D006842:Hydrocarbons, Brominated; D009942:Organometallic Compounds; D012996:Solutions; D014280:Triglycerides; C067783:ethyl magnesium bromide; C037311:bromoethane