Rapid detection of multidrug-resistant Mycobacterium tuberculosis in Cotonou (Benin) using two low-cost colorimetric methods: resazurin and nitrate reductase assays.
We have evaluated two simple, rapid and low-cost colorimetric methods for the detection of multidrug-resistant Mycobacterium tuberculosis. A total of 151 M. tuberculosis strains were tested for resistance to rifampicin (RMP) and isoniazid by resazurin microplate assay (REMA) and nitrate reductase assay (NRA) in comparison with the conventional proportion method (PM) on Löwenstein-Jensen medium. A complete agreement was found between NRA and PM, while one false RMP-susceptible result was found by REMA. REMA and NRA tests are rapid and inexpensive, and could be good alternatives to the conventional PM in low-resource countries.
Affolabi
Dissou
D
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.|Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
Sanoussi
N'Dira
N
Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
Odoun
Mathieu
M
Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
Martin
Anandi
A
Médecins Sans Frontières, 75011 Paris, France.|Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.
Koukpemedji
Louis
L
Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
Palomino
Juan Carlos
JC
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.
Kestens
Luc
L
Immnunology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.
Anagonou
Séverin
S
Laboratoire de Référence des Mycobactéries, BP 817, Cotonou, Bénin.
Portaels
Françoise
F
Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp 2000, Belgium.
D001426:Bacterial Proteins / Q000378:metabolism; D001541:Benin; D003124:Colorimetry; D024881:Drug Resistance, Bacterial; D018432:Drug Resistance, Multiple / Q000502:physiology*; D006801:Humans; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000201:enzymology / Q000302:isolation & purification*; D050901:Nitrate Reductase / Q000378:metabolism*; D012293:Rifampin / Q000494:pharmacology
D001426:Bacterial Proteins; D050901:Nitrate Reductase; D012293:Rifampin