[Study on technical processes for extraction and purification of total tanshinones].
OBJECTIVE
To optimize technical processes for extraction and purification of total tanshinones from Radix Salviae Miltiorrhizae.


METHODS
Using orthogonal design, and taking the extraction percentage of total tanshinones and tanshinone IIA as indexes, the extraction conditions of total tanshinones from Radix Salviae Miltiorrhizae and the purification conditions of total tanshinones from crude extract were optimized respectively.


RESULTS
The optimal technical process for extraction of total tanshinones was as follows: added 7 volumes of 90% aqueous ethanol, extracted three times and each time for 1.5 hours. The optimal technical process for purification of total tanshinones was as follows: 15 volumes of 8% sodium carbonate solution, washing 5 times and each time stirring for 5 minutes.


CONCLUSIONS
The content of total tanshinones in crude extract increased from 18.23% to 57.29% by purification. The optimal technical processes for extraction and purification of total tanshinones are practical in industrial production.
Zhang
Xiao-Xiang
XX
Department of Pharmaceutical Engineering, Hefei University of Technology, Hefei 230009, China. zxxwyfzsy@sina.com
Chen
Shao-Bing
SB
Wang
Yan-Ping
YP
D045784:Abietanes; D002851:Chromatography, High Pressure Liquid; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification; D000431:Ethanol / Q000737:chemistry; D010616:Phenanthrenes / Q000032:analysis / Q000302:isolation & purification*; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D015203:Reproducibility of Results; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D013997:Time Factors
D045784:Abietanes; D004365:Drugs, Chinese Herbal; D010616:Phenanthrenes; D012997:Solvents; C021751:tanshinone; D000431:Ethanol