title: Efficient antitumor immunity in a murine colorectal cancer model induced by CEA RNA-electroporated B cells.
abstract: RNA electroporation as a gene delivery method is more feasible and safer as compared with viral vectors. RNA-loaded dendritic cells (DC) have been used to induce T cell responses against tumor rejection antigens and B cells can also act as antigen-presenting cells for cellular vaccines. In this study, we compared B cells and DC, after electroporation with carcinoembryonic antigen (CEA) RNA, for their capacity to generate cytotoxic T lymphocytes and antitumor immunity. Vaccination using these B cells induced levels of IFN-gamma-secreting T cells and cytotoxic T cells comparable to those induced by DC. Intravenous administration was the optimum route for the B cell vaccine, while subcutaneous administration was the optimum route for the DC vaccine. The B cell vaccine predominantly generated CEA-specific CD4(+) T cells, whereas the DC vaccine generated CD8(+) T cells. Moreover, the B cell vaccine induced higher levels of anti-CEA antibodies than the DC vaccine. A heterogeneous prime-boost using B cells and DC failed to show any synergistic effects; however, the B cell vaccine did inhibit tumor growth and prolonged survival to a similar extent as the DC vaccine. Such RNA-electroporated B cells may prove useful as cellular tumor vaccines with potential clinical application.
authors.affiliation: Department of Microbiology and Immunology, Kang-Nam St Mary's Hospital, The Catholic University of Korea, Seoul, Republic of Korea.
authors.forename: Mi-Young
authors.initials: MY
authors.lastname: Park
authors.forename: Hye-Sung
authors.initials: HS
authors.lastname: Kim
authors.forename: Sun-Je
authors.initials: SJ
authors.lastname: Woo
authors.forename: Chang-Hyun
authors.initials: CH
authors.lastname: Kim
authors.forename: Jung-Sun
authors.initials: JS
authors.lastname: Park
authors.forename: Hyun-Jung
authors.initials: HJ
authors.lastname: Sohn
authors.forename: Hyung-Jin
authors.initials: HJ
authors.lastname: Kim
authors.forename: Seong-Taek
authors.initials: ST
authors.lastname: Oh
authors.forename: Tai-Gyu
authors.initials: TG
authors.lastname: Kim
mesh_terms: D000818:Animals; D017951:Antigen Presentation; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D019496:Cancer Vaccines / Q000276:immunology*; D002272:Carcinoembryonic Antigen / Q000276:immunology*; D015179:Colorectal Neoplasms / Q000276:immunology* / Q000628:therapy; D003713:Dendritic Cells / Q000276:immunology; D004195:Disease Models, Animal; D018274:Electroporation; D005260:Female; D018014:Gene Transfer Techniques; D051379:Mice; D008810:Mice, Inbred C57BL; D012313:RNA / Q000378:metabolism*; D014611:Vaccination
chemical_list: D019496:Cancer Vaccines; D002272:Carcinoembryonic Antigen; D012313:RNA