One-step rapid determination and purification of puerarin from Radix puerariae by n-octylamine-modified poly(methacrylate-co-ethylene dimethacrylate) monolith.
n-Octylamine-modified poly(methacrylate-co-ethylene dimethacrylate) monoliths were prepared for rapid screening, determination and one-step purification of puerarin from Radix puerariae (a crude extract of the root of Pueraria lobata). The modified monolith showed a specific surface area of 17.8 m(2) g(-1), an average pore size of 0.76 microm and a total porosity of 60.8%. Fast separation of R. puerariae crude extract was achieved within 5 min at a flow velocity of 722 cm h(-1) resulting in a puerarin purity of 97%, with a recovery of 85%. This demonstrates the potential of n-octylamine-modified poly(methacrylate-co-ethylene dimethacrylate) monolith for the rapid analysis and separation of isoflavonoids. Preparative scale sample loading (12 mg in 2 mL) resulted in a purity of 95%, and a recovery of about 69%. HPLC, FTIR, MS and (1)H NMR spectroscopy were used for the characterization and quantification of puerarin in isolated fraction.
Lv
Yong-Qin
YQ
College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China. yongqinlv.student@sina.com <yongqinlv.student@sina.com>
Tan
Tian-Wei
TW
Wang
Man-Yi
MY
Janson
Jan-Christer
JC
D000588:Amines / Q000737:chemistry; D002853:Chromatography, Liquid / Q000379:methods*; D007529:Isoflavones / Q000302:isolation & purification*; D008689:Methacrylates / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D029908:Pueraria / Q000737:chemistry*
D000588:Amines; D007529:Isoflavones; D008689:Methacrylates; C008699:octylamine; C033607:puerarin