title: Cell size distribution as a parameter for the predetermination of exponential growth during repeated batch cultivation of CHO cells.
abstract: The routine measurement of the cell size distribution of a Chinese hamster ovary (CHO) cell population during a repeated batch process enables the predetermination of exponential growth even 24 h before the population enters the log phase, due to a short but significantly increased cell size during the lag phase. A prolongation of the stationary phase causes to progressive limitation in asparagine, serine, and ethanolamine. Such extended limitation influences the duration of the following lag phase and obviously induces a synchronization of the cell population that can be monitored easily by a fast cell size analyzing technique. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 793-797, 1997.
authors.affiliation: Institute of Cell Culture Technology, Faculty of Technical Sciences, University of Bielefeld, D-33501 Bielefeld, Germany. tse@zellkult.techfak.uni-bielefeld.de
authors.forename: T
authors.initials: T
authors.lastname: Seewöster
authors.forename: J
authors.initials: J
authors.lastname: Lehmann