Involvement of the host RNA polymerase in the early transcription program of Caulobacter crescentus bacteriophage phiCdl DNA.
The host RNA polymerase appears to be involved in the early transcription program of the Caulobacter crescentus bacteriophage phiCdl. The addition of rifampicin early after infection inhibited the production of phage, whereas phiCdl production was not inhibited by the addition of rifampicin at any time after infection of a rifampicin-resistant host. In addition, we found that a rifampicin-resistant RNA polymerase activity dependent on de novo protein synthesis is required for late transcription. The region of early phiCdl was mapped by hybridizing labeled RNA extracted from phiCdl-infected cells grown in the presence or absence of chloramphenicol to HindIII and HpaI restriction fragments of the phiCdl genome.
Amemiya
K
K
Department of Molecular Biology, Division of Biological Sciences, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
Raboy
B
B
Shapiro
L
L