title: The genome of a baculovirus infecting Tipula paludosa (Meig) (diptera): a high molecular weight closed circular DNA of zero superhelix density.
abstract: A characterization of the genome of a baculovirus infecting the diptera, Tipula paludosa Meig, is presented here. It appears to be a double-stranded closed circular DNA. The supercoiled structure has been visualized by electron microscopy. The relaxed molecules have a contour length of 51.2 +/- 1.5 mum which corresponds to a molecular weight of 92 x 10(6) daltons, by taking the value of 6.4 x 10(6) daltons for PM2 DNA used as an internal standard for the spreading. T. paludosa baculovirus DNA has a low G + C content of 27%, as estimated from its buoyant density in CsCl, uv spectrum, and melting temperature. Its sedimentation coefficient in alkaline solution was 294 S, as determined by analytical sedimentation.
authors.affiliation: Institut de Recherches Scientifiques sur le Cancer, B.P. No. 8, 9 800 Villejuif, France.
authors.forename: B M
authors.initials: BM
authors.lastname: Revet
authors.forename: B
authors.initials: B
authors.lastname: Guelpa