title: Surfactant enhancement of (S)-naproxen ester productivity from racemic naproxen by lipase in isooctane.
abstract: In the enantioselective esterification of racemic Naproxen with trimethylsilyl methanol in isooctane by Candida cylindracea lipase, improvements in (S)-naproxen ester productivity and enzyme selectivity were demonstrated by adding bis(2-ethylhexyl) sodium sulfosuccinate (AOT) as the best surfactant. The effect of water content on the enhancement of enzyme activity was elucidated from the reduced adsorption of surfactant molecules on the lipase. A competitive inhibition by the alcohol and a noncompetitive inhibition by the surfactant to the enzyme were found from the kinetic analysis. By using a two-phase extraction, a complete separation of the surfactant from the organic solution was obtained.
authors.affiliation: Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan, 70101. Republic of China.
authors.forename: S W
authors.initials: SW
authors.lastname: Tsai
authors.forename: C C
authors.initials: CC
authors.lastname: Lu
authors.forename: C S
authors.initials: CS
authors.lastname: Chang