High cell density fermentation of recombinant Vibrio cholerae for the production of B subunit of Escherichia coli enterotoxin.
High cell density fermentation studies were performed to produce the B subunit of Escherichia coli heat-labile enterotoxin (LTB) from a Vibrio cholerae culture that carries a recombinant plasmid with an ampicillin resistance gene, tac promoter, and the gene encoding LTB. Upon induction with isopropyl-beta-D-thiogalactopyranoside (IPTG) the culture secreted the protein into the extracellular milieu. Fed-batch fermentation with stepwise addition of a total of 5 mM of IPTG during the active growth phase of the organism resulted in the production of 400 mg/L of LTB in 9 h and a cell optical density (OD) of 24. The LTB was purified to homogeneity with 70% recovery from the fermentation broth and was found to be chemically and biologically identical to the native protein by N-terminal amino acid sequencing and receptor binding assay. (c) 1995 John Wiley & Sons, Inc.
Panda
A K
AK
National institute of immunology, Aruna Asaf Ali Marg, New Delhi 110067, India.
Ghorpade
A
A
Mukhopadhyay
A
A
Talwar
G P
GP
Garg
L C
LC