Lactate metabolism in the muscle of the crab Chasmagnathus granulatus during hypoxia and post-hypoxia recovery.
The present study showed that the lactate/glucose ratio in the hemolymph of Chasmagnathus granulatus maintained in normoxia (controls) was 4.9, suggesting that lactate is an important substrate for this crab. Periods of hypoxia are part of the biological cycle of this crab, and lactate is the main end product of anaerobiosis in this crab. Our hypothesis was that this lactate would be, therefore, used by gluconeogenic pathway or can be oxidized or excreted to the aquatic medium during hypoxia and post-hypoxia periods in C. granulatus. The concentrations of hemolymphatic lactate in animals in normoxia are high, and are used as an energy substrate. In hypoxia, muscle gluconeogenesis and excretion of lactate to the aquatic medium would contribute significantly in regulating the concentration of circulating lactate. Utilization of these pathways would serve the objective of maintaining the acid-base equilibrium of the organism. Muscle gluconeogenesis participates, during the recovery process, in metabolizing the lactate produced during the period of hypoxia. Lactate excretion to the external medium, was one of the strategies used to decrease the higher hemolymphatic lactate levels. However, oxidation of lactate in the muscle is not a main strategy used by this crab to metabolize lactate in the recovery periods.
Maciel
José Eduardo S
JE
Department of Physiology, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, 90050-170 Porto Alegre, RS, Brazil.
Souza
Fabiana
F
Valle
Sandra
S
Kucharski
Luiz Carlos
LC
da Silva
Roselis Silveira Martins
RS
D000693:Anaerobiosis; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D003386:Brachyura / Q000378:metabolism*; D002247:Carbon Isotopes; D005943:Gluconeogenesis; D006003:Glycogen / Q000378:metabolism; D006458:Hemolymph / Q000378:metabolism; D007568:Jaw / Q000378:metabolism; D019344:Lactic Acid / Q000097:blood / Q000378:metabolism*; D008297:Male; D009132:Muscles / Q000378:metabolism*; D010084:Oxidation-Reduction
D001786:Blood Glucose; D002247:Carbon Isotopes; D019344:Lactic Acid; D006003:Glycogen