title: Peripheral blood mononuclear cell infiltration and neuroinflammation in the HexB-/- mouse model of neurodegeneration.
abstract: Myeloid-derived immune cells, including microglia, macrophages and monocytes, have been previously implicated in neurodegeneration. We investigated the role of infiltrating peripheral blood mononuclear cells (PBMC) in neuroinflammation and neurodegeneration in the HexB-/- mouse model of Sandhoff disease. Ablation of the chemokine receptor CCR2 in the HexB-/- mouse resulted in significant inhibition of PBMC infiltration into the brain, decrease in TNFalpha and MHC-II mRNA abundance and retardation in clinical disease development. There was no change in the level of GM2 storage and pro-apoptotic activity or astrocyte activation in HexB-/-; Ccr2-/- double knockout mice, which eventually succumbed secondary to GM2 gangliosidosis.
authors.affiliation: Department of Neurobiology & Anatomy, School of Medicine & Dentistry, University of Rochester Rochester NY 14642, USA. Stephanos_kyrkanides@urmc.rochester.edu
authors.forename: Stephanos
authors.initials: S
authors.lastname: Kyrkanides
authors.forename: Ann W
authors.initials: AW
authors.lastname: Miller
authors.forename: Jen-Nie H
authors.initials: JN
authors.lastname: Miller
authors.forename: Ross H
authors.initials: RH
authors.lastname: Tallents
authors.forename: Sabine M
authors.initials: SM
authors.lastname: Brouxhon
authors.forename: Mallory E
authors.initials: ME
authors.lastname: Olschowka
authors.forename: M Kerry
authors.initials: MK
authors.lastname: O'Banion
authors.forename: John A
authors.initials: JA
authors.lastname: Olschowka
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000276:immunology; D004195:Disease Models, Animal; D004660:Encephalitis / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D005260:Female; D005678:G(M2) Ganglioside / Q000378:metabolism; D054819:Hexosaminidase B / Q000235:genetics / Q000276:immunology*; D007963:Leukocytes, Mononuclear / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D018345:Mice, Knockout; D017628:Microglia / Q000276:immunology / Q000473:pathology; D009410:Nerve Degeneration / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D054390:Receptors, CCR2 / Q000235:genetics / Q000276:immunology; D012497:Sandhoff Disease / Q000276:immunology* / Q000378:metabolism / Q000473:pathology
chemical_list: C515335:Ccr2 protein, mouse; D054390:Receptors, CCR2; D005678:G(M2) Ganglioside; D054819:Hexosaminidase B