title: The effects of binge alcohol exposure in the 2nd trimester on the estimated density of cerebral microvessels in near-term fetal sheep.
abstract: Heavy fetal alcohol exposure is associated with a spectrum of neurological abnormalities, although the mechanism of injury is largely unknown. We previously reported attenuated cerebral blood flow response to hypoxia in fetal and newborn sheep which were exposed to alcohol earlier in pregnancy. One possible mechanism for this effect of alcohol on the developing cerebral vasculature is a decrease in cerebral microvessel density, similar to its effect on developing neurons. Therefore, we tested the hypothesis that prenatal alcohol exposure decreases cerebral microvessel density. Pregnant ewes received intravenous infusions of ethanol or saline during days 60-84 of gestation (term=150 days) and at 125 days of gestation we obtained the fetal brains for study. We immunohistochemically labeled vessels of the left cerebral forebrain hemispheres with antibody to endothelial nitric oxide synthase and then obtained unbiased stereological estimates of cerebral microvessel density using a modified optical fractionator method. We studied 20 fetal brains of which 9 were alcohol-exposed, 11 were saline-controls, and all were products of a twin gestation. Although brain and body weights were not different between groups, the alcohol-exposed group had significantly lower brain weight as a percentage of body weight. Estimates of cerebral microvessel density were not significantly different between alcohol-exposed and saline-control groups: 12.7+/-8.7 and 9.1+/-2.8 microvessels per mm(3), respectively (mean+/-SD, p=0.32). Since there is no change in estimated cerebral microvessel density after prenatal alcohol exposure, we conclude that decreased cerebral microvessel density is not a likely explanation for attenuated cerebral blood flow in response to hypoxia.
authors.affiliation: Department of Pediatrics, University of Washington, Seattle, WA, USA.
authors.forename: Katherine E
authors.initials: KE
authors.lastname: Simon
authors.forename: Robin L
authors.initials: RL
authors.lastname: Mondares
authors.forename: Donald E
authors.initials: DE
authors.lastname: Born
authors.forename: Christine A
authors.initials: CA
authors.lastname: Gleason
mesh_terms: D020268:Alcohol-Induced Disorders, Nervous System / Q000473:pathology* / Q000503:physiopathology; D000437:Alcoholism / Q000150:complications; D000818:Animals; D001160:Arterioles / Q000002:abnormalities / Q000187:drug effects / Q000503:physiopathology; D002492:Central Nervous System Depressants / Q000633:toxicity; D002536:Cerebral Arteries / Q000002:abnormalities* / Q000187:drug effects* / Q000503:physiopathology; D002560:Cerebrovascular Circulation / Q000187:drug effects; D004195:Disease Models, Animal; D000431:Ethanol / Q000633:toxicity; D005260:Female; D063647:Fetal Alcohol Spectrum Disorders / Q000473:pathology* / Q000503:physiopathology; D002534:Hypoxia, Brain / Q000503:physiopathology; D008297:Male; D008833:Microcirculation / Q000187:drug effects*; D018919:Neovascularization, Physiologic / Q000187:drug effects; D052250:Nitric Oxide Synthase Type III / Q000187:drug effects / Q000378:metabolism; D009929:Organ Size / Q000187:drug effects; D011247:Pregnancy; D011262:Pregnancy Trimester, Second / Q000187:drug effects; D016548:Prosencephalon / Q000098:blood supply / Q000187:drug effects / Q000503:physiopathology; D012756:Sheep
chemical_list: D002492:Central Nervous System Depressants; D000431:Ethanol; D052250:Nitric Oxide Synthase Type III