title: Isolation of dibenzofuran-degrading bacterium, Nocardioides sp. DF412, and characterization of its dibenzofuran degradation genes.
abstract: A dibenzofuran (DF)-degrading bacterium, Nocardioides sp. DF412, was isolated and its genes responsible for the degradation of DF were cloned and characterized. The dfdA1A2A3A4 gene cluster coding for a ring hydroxylating dioxygenase was located on a plasmid, and the dfdBC gene cluster coding for a ring-cleavage dioxygenase and a following hydrolase respectively was located on the chromosome. Reverse transcription-PCR analysis revealed that the transcription of both gene clusters was induced in the presence of DF. These two gene clusters also conferred the activity of each enzyme to a host strain. These results strongly suggest that the clusters are responsible for the degradation of DF in strain DF412.
authors.affiliation: Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan. kmiya@tjcc.tohoku-gakuin.ac.jp
authors.forename: Keisuke
authors.initials: K
authors.lastname: Miyauchi
authors.forename: Parichat
authors.initials: P
authors.lastname: Sukda
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Nishida
authors.forename: Emi
authors.initials: E
authors.lastname: Ito
authors.forename: Yoshikatsu
authors.initials: Y
authors.lastname: Matsumoto
authors.forename: Eiji
authors.initials: E
authors.lastname: Masai
authors.forename: Masao
authors.initials: M
authors.lastname: Fukuda
mesh_terms: D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001572:Benzofurans / Q000378:metabolism*; D001673:Biodegradation, Environmental
chemical_list: D001426:Bacterial Proteins; D001572:Benzofurans; C023614:dibenzofuran