Secretome-based identification of Mac-2 binding protein as a potential oral cancer marker involved in cell growth and motility.
Oral squamous cell carcinoma (OSCC) is the 11th most common cancer worldwide, and is associated with a high death rate. At present, there are no suitable markers for detecting and/or monitoring OSCC in body fluids/tissues. Here, we used 1D SDS-PAGE and MALDI-TOF MS to systematically analyze the secretomes of two OSCC cell lines (OEC-M1 and SCC4). The putative OSCC-related proteins identified in this analysis included the Mac-2 binding protein (Mac-2 BP), which was further found to be overexpressed in OSCC specimens and significantly elevated in the sera of OSCC patients compared to healthy controls. Finally, RNA interference-based knock-down of Mac-2 BP expression in OSCC cells revealed for the first time that Mac-2 BP is involved in regulating growth and motility of OSCC cells.
Weng
Li-Ping
LP
Graduate Institute of Biomedical Sciences, Proteomics Core Laboratory, Molecular Medicine Research Center, Department of Cell and Molecular Biology, Department of Public Health, Chang Gung University, Tao-Yuan, Taiwan.
Wu
Chih-Ching
CC
Hsu
Bao-Lian
BL
Chi
Lang-Ming
LM
Liang
Ying
Y
Tseng
Ching-Ping
CP
Hsieh
Ling-Ling
LL
Yu
Jau-Song
JS
D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000032:analysis* / Q000097:blood; D001483:Base Sequence; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000097:blood; D002294:Carcinoma, Squamous Cell / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle; D002455:Cell Division; D002465:Cell Movement; D017077:Culture Media, Conditioned; D004591:Electrophoresis, Polyacrylamide Gel; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000032:analysis* / Q000097:blood; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000175:diagnosis / Q000378:metabolism* / Q000473:pathology; D020543:Proteome; D034622:RNA Interference
D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D017077:Culture Media, Conditioned; D008562:Membrane Glycoproteins; D020543:Proteome; C114782:TAA90K protein, human