Rosiglitazone-enriched diet did not protect liver ischemia-reperfusion injury in a rat model.
OBJECTIVE
To determine whether rosiglitazone-enriched diet offer protection in a classical model of liver ischemia-reperfusion injury in rats.


METHODS
Two days before the experiment, rats were divided into 2 groups: Control Group (n=13) rats fed with standard diet; Rosi Group (n=13): rats fed with a powdered standard diet supplemented with rosiglitazone. The animals were submitted to liver ischemia-reperfusion by clamping the pedicle of median and left anterolateral lobes. After 1 hour of partial hepatic ischemia, the clamp was removed for reperfusion. After 2 or 24 hours (Control and Rosi Groups), blood was collected for enzymes and cytokines analysis. Ischemic and non-ischemic liver were collected for malondialdehyde analysis and histological assessment. Lungs were removed for tissue myeloperoxidase quantification.


RESULTS
There were no statistical differences between groups for all analysed parameters.


CONCLUSIONS
In this model, rosiglitazone-enriched diet did not protect liver against ischemia-reperfusion injury.
Teixeira
Antonio Roberto Franchi
AR
Transplantation Division, Department of Gastroenterology, School of Medicine, USP, São Paulo, Brazil. drrteixeira@uol.com.br
Molan
Nilza Trindade
NT
Bellodi-Privato
Marta
M
Coelho
Ana Maria
AM
Leite
Kátia Ramos
KR
Seguro
Antônio Carlos
AC
Bacchella
Telésforo
T
Machado
Marcel Cerqueira César
MC
D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D016207:Cytokines / Q000097:blood; D019587:Dietary Supplements; D004195:Disease Models, Animal; D004353:Drug Evaluation, Preclinical; D008099:Liver / Q000098:blood supply* / Q000187:drug effects / Q000201:enzymology; D008297:Male; D047495:PPAR gamma / Q000008:administration & dosage*; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000473:pathology / Q000517:prevention & control*; D000077154:Rosiglitazone; D045162:Thiazolidinediones / Q000008:administration & dosage*
D016207:Cytokines; D047495:PPAR gamma; D045162:Thiazolidinediones; D000077154:Rosiglitazone; D001219:Aspartate Aminotransferases