Effects of acute temperature or salinity stress on the immune response in sea cucumber, Apostichopus japonicus.
Invertebrates are increasingly raised in mariculture, where it is important to monitor immune function and to minimize stresses that could suppress immunity. The activities of phagocytosis, superoxide dismutase (SOD), catalase (CAT), myeloperoxidase (MPO), and lysozyme (LSZ) were measured to evaluate the immune capacities of the sea cucumber, Apostichopus japonicus, to acute temperature changes (from 12 degrees C to 0 degrees C, 8 degrees C, 16 degrees C, 24 degrees C, and 32 degrees C for 72 h) and salinity changes (from 30 per thousand to 20 per thousand, 25 per thousand, and 35 per thousand for 72 h) in the laboratory. Phagocytosis was significantly affected by temperature increases in 3 h, and by salinity (25 per thousand and 35 per thousand) changes in 1 h. SOD activities decreased significantly in 0.5 h to 6 h samples at 24 degrees C. At 32 degrees C, SOD activities decreased significantly in 0.5 h and 1 h exposures, and obviously increased for 12 h exposure. CAT activities decreased significantly at 24 degrees C for 0.5 h exposure, and increased significantly at 32 degrees C in 3 h to 12 h exposures. Activities of MPO increased significantly at 0 degrees C in 0.5 h to 6 h exposures and at 8 degrees C for 1 h. By contrast, activities of MPO decreased significantly in 24 degrees C and 32 degrees C treatments. In elevated-temperature treatments, activities of LSZ increased significantly except at 32 degrees C for 6 h to 12 h exposures. SOD activity was significantly affected by salinity change. CAT activity decreased significantly after only 1 h exposure to salinity of 20 per thousand. Activities of MPO and LSZ showed that A. japonicus tolerates limited salinity stress. High-temperature stress had a much greater effect on the immune capacities of A. japonicus than did low-temperature and salinity stresses.
Wang
Fangyu
F
Institute of Oceanology, Chinese Academy of Sciences, Qingdao, PR China; Graduate School, Chinese Academy of Sciences, Beijing, PR China.
Yang
Hongsheng
H
Gao
Fei
F
Liu
Guangbin
G
D000818:Animals; D002374:Catalase / Q000378:metabolism; D006434:Hemocytes / Q000276:immunology / Q000502:physiology; D009113:Muramidase / Q000378:metabolism; D009195:Peroxidase / Q000378:metabolism; D010587:Phagocytosis; D054712:Salinity; D012616:Sea Cucumbers / Q000276:immunology* / Q000502:physiology; D013312:Stress, Physiological; D013482:Superoxide Dismutase / Q000378:metabolism; D013696:Temperature
D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D009113:Muramidase