Determination of betamethasone in human plasma by liquid chromatography with tandem mass.
A sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of betamethasone in human plasma. The analyte was isocratically eluted on a Venusil XBP C8 column (200 mm x 3.9 mm ID, 5 microm) with methanol-water mol x L(-1) ammonium formate) (80:20) at a flow rate of 0.4 mL x min(-1), and detected (containing 5 mmol x L(-1) ammonium formate) (80:20) at a flow rate of 0.4 mL x min(-1), and detected with a triple quad LC-MS/MS using ESI with positive ionization. Ions monitored in the multiple reaction monitoring (MRM) mode were m/z 393.3-->355.2 for betamethasone and m/z 361.3-->343.2 for prednisolone (IS). Betamethasone was extracted from 0.5 mL human plasma with ethyl acetate. The average recovery is 88.24% and the low limit of quantitation (LLOQ) was 0.5 ng x mL(-1). The 3-day validation study demonstrated excellent precision and accuracy across the calibration range of 0.5-80.0 ng x mL(-1). The method was successfully applied to the pharmacokinetic study of compound betamethason injection in healthy Chinese volunteers.
Qu
Ting-Ting
TT
Institute of Clinical Pharmacology, Qilu Hospital of Shandong University, Jinan 250012, China.
Zhang
Rui
R
Wang
Ben-Jie
BJ
Liu
Xiao-Yan
XY
Yuan
Gui-Yan
GY
Guo
Rui-Chen
RC
D000893:Anti-Inflammatory Agents / Q000097:blood* / Q000493:pharmacokinetics; D019540:Area Under Curve; D001623:Betamethasone / Q000097:blood* / Q000493:pharmacokinetics; D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007273:Injections, Intramuscular; D008297:Male; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods*; D055815:Young Adult
D000893:Anti-Inflammatory Agents; D001623:Betamethasone