[Study of nHACP/CF co-cultured with osteoblasts in vitro].
OBJECTIVE
To investigate the effect of nHACP/CF on coherence and proliferation of osteoblast.


METHODS
The rat osteoblasts were isolated and cultured, then co-cultured with nHACP/CF in vitro. The cells were observed under cytochemical staining for ALP; the cell-material complex was observed under electronic scanning microscope, MTT was used for detection of cell proliferation,and ALP activity was determined at different times.SPSS 11.5 software package was used for one-way ANOVA.


RESULTS
Cytochemical staining for ALP was positive. The best density of seeding cells was 6x10(5)/ml; osteoblast could attach and extend on nHACP/CF,the proliferation and growth were not affected by nHACP/CF.


CONCLUSIONS
nHACP/CF is a good vehicle for rat osteoblast to grow on.
Sun
Ning-Ning
NN
Department of Oral and Maxillofacial Surgery, School of Stomatology,China Medical University. Shenyang 110002, Liaoning Province, China. clare_ning@yahoo.com.cn
Wang
Xu-Kai
XK
Zhou
Qing
Q
Lu
Li
L
Huang
Shao-Hui
SH
D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D066298:In Vitro Techniques; D010006:Osteoblasts; D051381:Rats
D003470:Culture Media