Tolerance of combined submergence and salinity in the halophytic stem-succulent Tecticornia pergranulata.
OBJECTIVE
Habitats occupied by many halophytes are not only saline, but are also prone to flooding. Few studies have evaluated submergence tolerance in halophytes.


METHODS
Responses to submergence, at a range of salinity levels, were studied for the halophytic stem-succulent Tecticornia pergranulata subsp. pergranulata (syn. Halosarcia pergranulata subsp. pergranulata). Growth and total sugars in succulent stems were assessed as a function of time after submergence. Underwater net photosynthesis, dark respiration, total sugars, glycinebetaine, Na(+), Cl(-) and K(+), in succulent stems, were assessed in a NaCl dose-response experiment.


RESULTS
Submerged plants ceased to grow, and tissue sugars declined. Photosynthesis by succulent stems was reduced markedly when underwater, as compared with in air. Capacity for underwater net photosynthesis (P(N)) was not affected by 10-400 mM NaCl, but it was reduced by 30 % at 800 mM. Dark respiration, underwater, increased in succulent stems at 200-800 mM NaCl, as compared with those at 10 mM NaCl. On an ethanol-insoluble dry mass basis, K(+) concentration in succulent stems of submerged plants was equal to that in drained controls, across all NaCl treatments. Na(+) and Cl(-) concentrations, however, were elevated in stems of submerged plants, but so was glycinebetaine. Submerged stems increased in succulence, so solutes would have been 'diluted' on a tissue-water basis.


CONCLUSIONS
Tecticornia pergranulata tolerates complete submergence, even in waters of high salinity. A 'quiescence response', i.e. no shoot growth, would conserve carbohydrates, but tissue sugars still declined with time. A low K(+) : Na(+) ratio, typical for tissues of succulent halophytes, was tolerated even during prolonged submergence, as evidenced by maintenance of underwater P(N) at up to 400 mM NaCl. Underwater P(N) provides O(2) and sugars, and thus should enhance survival of submerged plants.
Colmer
T D
TD
School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
Vos
H
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Pedersen
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D000222:Adaptation, Physiological / Q000187:drug effects; D027703:Amaranthaceae / Q000166:cytology / Q000254:growth & development / Q000502:physiology*; D001622:Betaine / Q000494:pharmacology; D018533:Biomass; D050260:Carbohydrate Metabolism / Q000187:drug effects; D019069:Cell Respiration / Q000187:drug effects; D017753:Ecosystem; D055868:Floods; D007477:Ions; D010788:Photosynthesis / Q000187:drug effects; D018547:Plant Stems / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D054712:Salinity; D055051:Salt-Tolerant Plants / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D012965:Sodium Chloride / Q000494:pharmacology; D013997:Time Factors
D007477:Ions; D001622:Betaine; D012965:Sodium Chloride