Identification of novel retromer complexes in the mouse testis.
A family of vacuolar protein sorting (Vps) proteins, which are components of mammalian retromer complex, has been studied in the mouse. Vps26a is known as a retromer component that plays an important role in embryonic development: however, its cell-type expression and precise role remain to be elucidated. In this study, we identified a new isoform of Vps26a, called Vps26aT, which was expressed specifically in the mouse testis. Diverse expression patterns of Vps26 variants in mouse tissues were determined by Western blot and RT-PCR analyses, and the direct interaction of Vps26aT with Vps35 was also demonstrated by immunoprecipitation and pull-down assay using antibodies raised against each Vps component. Our results revealed that the retromer complex could be formed from different Vps26 isoforms in a tissue-specific manner, resulting in more than two types of the retromer complex, including the Vps26a-Vps29-Vps35, Vps26aT-Vps29-Vps35, and Vps26b-Vps29-Vps35 complexes in mouse tissues.
Kim
Ekyune
E
National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, 685-1 Yangcheong-ri, Ochang-eup, Chung-buk 363-883, Republic of Korea.
Lee
Jae-Woong
JW
Baek
Dong-Chul
DC
Lee
Sang-Rae
SR
Kim
Myeong-Su
MS
Kim
Sang-Hyun
SH
Imakawa
Kazuhiko
K
Chang
Kyu-Tae
KT
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D000818:Animals; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D014018:Tissue Distribution; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
D020033:Protein Isoforms; D033921:Vesicular Transport Proteins; C089989:Vps26 protein, mouse; C101274:Vps35 protein, mouse