title: Purification and determination of the chemical structure of the tyrosinase inhibitor produced by Trichoderma viride strain H1-7 from a marine environment.
abstract: Tyrosinase is a key enzyme in the synthesis of melanin and is widely distributed in animals, plants, and microorganisms. As excessive melanin production causes not only hyperpigmenting effects on human skin but also melanosis in various foods, an inhibitor of tyrosinase has become of interest lately from a practical point of view. In the present study, we purified the tyrosinase inhibitor produced by Trichoderma viride strain H1-7 from a marine environment. The purified inhibitor showed a single peak on HPLC. The chemical structure of this compound was determined by NMR and mass spectrometry analyses. The structure was the same as homothallin II that has been isolated as an antibiotic from T. koningii and T. harzianum. The inhibitor showed competitive inhibition against mushroom tyrosinase.
authors.affiliation: Department of Microbiology, Osaka University of Pharmaceutical Sciences, Osaka, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Tsuchiya
authors.forename: Katsuhisa
authors.initials: K
authors.lastname: Yamada
authors.forename: Katsuhiko
authors.initials: K
authors.lastname: Minoura
authors.forename: Katsushiro
authors.initials: K
authors.lastname: Miyamoto
authors.forename: Yoshihide
authors.initials: Y
authors.lastname: Usami
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kobayashi
authors.forename: Naoko
authors.initials: N
authors.lastname: Hamada-Sato
authors.forename: Chiaki
authors.initials: C
authors.lastname: Imada
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tsujibo
mesh_terms: D003517:Cyclopentanes / Q000737:chemistry / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D007700:Kinetics; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors*; D014242:Trichoderma / Q000737:chemistry / Q000378:metabolism*; D014871:Water Microbiology
chemical_list: D003517:Cyclopentanes; D004791:Enzyme Inhibitors; C090065:homothallin II; D014442:Monophenol Monooxygenase