title: A Role for galectin-3 in renal tissue damage triggered by ischemia and reperfusion injury.
abstract: Ischemic-reperfusion injury (IRI) triggers an inflammatory response involving neutrophils/macrophages, lymphocytes and endothelial cells. Galectin-3 is a multi-functional lectin with a broad range of action such as promotion of neutrophil adhesion, induction of oxidative stress, mastocyte migration and degranulation, and production of pro-inflammatory cytokines. The aim of this study was evaluate the role of galectin-3 in the inflammation triggered by IRI. Galectin-3 knockout (KO) and wild type (wt) mice were subjected to 45 min of renal pedicle occlusion. Blood and kidney samples were collected at 6, 24, 48 and 120 h. Blood urea was analyzed enzymatically, while MCP-1, IL-6 and IL-1beta were studied by real-time PCR. Reactive oxygen species (ROS) was investigated by flow cytometry. Morphometric analyses were performed at 6, 24, 48 and 120 h after reperfusion. Urea peaked at 24 h, being significantly lower in knockout animals (wt = 264.4 +/- 85.21 mg/dl vs. gal-3 KO = 123.74 +/- 29.64 mg/dl, P = 0.001). Galectin-3 knockout animals presented less acute tubular necrosis and a more prominent tubular regeneration when compared with controls concurrently with lower expression of MCP-1, IL-6, IL-1beta, less macrophage infiltration and lower ROS production at early time points. Galectin-3 seems to play a role in renal IRI involving the secretion of macrophage-related chemokine, pro-inflammatory cytokines and ROS production.
authors.affiliation: Laboratory of Clinical and Experimental Immunology, Division of Nephrology, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Fernandes Bertocchi
authors.forename: Gabriela
authors.initials: G
authors.lastname: Campanhole
authors.forename: Pamella Huey Mei
authors.initials: PH
authors.lastname: Wang
authors.forename: Giselle Martins
authors.initials: GM
authors.lastname: Gonçalves
authors.forename: Márcio José
authors.initials: MJ
authors.lastname: Damião
authors.forename: Marcos Antônio
authors.initials: MA
authors.lastname: Cenedeze
authors.forename: Felipe Caetano
authors.initials: FC
authors.lastname: Beraldo
authors.forename: Vicente
authors.initials: V
authors.lastname: de Paula Antunes Teixeira
authors.forename: Marlene Antônia
authors.initials: MA
authors.lastname: Dos Reis
authors.forename: Marilda
authors.initials: M
authors.lastname: Mazzali
authors.forename: Alvaro
authors.initials: A
authors.lastname: Pacheco-Silva
authors.forename: Niels Olsen Saraiva
authors.initials: NO
authors.lastname: Câmara
mesh_terms: D000818:Animals; D001324:Autoantigens; D015415:Biomarkers; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000235:genetics; D004247:DNA / Q000235:genetics*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005500:Follow-Up Studies; D037502:Galectin 3 / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D007668:Kidney / Q000098:blood supply*; D016030:Kidney Transplantation / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
chemical_list: D001324:Autoantigens; D015415:Biomarkers; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D037502:Galectin 3; D053583:Interleukin-1beta; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D004247:DNA