title: Determination and characterization of new benzimidazoles with activity against Trypanosoma cruzi by UV spectroscopy and HPLC.
abstract: This work presents the development of analytical methodologies by UV spectrophotometry and HPLC to characterize five nitroarylbenzimidazole derivatives with activity against Trypanosoma cruzi: NB, BNB, PNB, PMNB and PCNB. Both methodologies exhibit adequate repeatabilities and reproducibilities (CV<2%) and recoveries higher than 98%. The ionization constants (pK(a)), lipophilicity (log P) and effective permeability (Pe) are reported. The five compounds present an inhibitory effect on the T. cruzi growth (epimastigotes) at 1-100 microM concentration range in an order rank of PMNB>PCNB>PNB>BNB>NB. Additionally, cyclic voltammetric data reveal that the nitroarylbenzimidazole derivatives might sustain their effects on growth and oxygen uptake on T. cruzi epimastigotes.
authors.affiliation: Chemical and Pharmaceutical Sciences Faculty, University of Chile. P.O. Box 233, Santiago 1, Chile.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Brain-Isasi
authors.forename: Cristián
authors.initials: C
authors.lastname: Quezada
authors.forename: Hernán
authors.initials: H
authors.lastname: Pessoa
authors.forename: Antonio
authors.initials: A
authors.lastname: Morello
authors.forename: Marcelo J
authors.initials: MJ
authors.lastname: Kogan
authors.forename: Alejandro
authors.initials: A
authors.lastname: Alvarez-Lueje
mesh_terms: D000818:Animals; D001562:Benzimidazoles / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D004563:Electrochemistry; D007700:Kinetics; D013056:Spectrophotometry, Ultraviolet; D014344:Trypanocidal Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D001562:Benzimidazoles; D014344:Trypanocidal Agents