Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription.
We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.
Furukawa
Kazuhiro
K
Nano Medical Engineering Laboratory, Advanced Science Institute, RIKEN 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
Abe
Hiroshi
H
Abe
Naoko
N
Harada
Mitsuru
M
Tsuneda
Satoshi
S
Ito
Yoshihiro
Y
D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005456:Fluorescent Dyes / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010766:Phosphorylation; D012313:RNA / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D012394:Rosaniline Dyes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D020080:Tandem Repeat Sequences; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism
D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D012394:Rosaniline Dyes; D014764:Viral Proteins; C005095:malachite green; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases