Film thickness dependence of protein adsorption from blood serum and plasma onto poly(sulfobetaine)-grafted surfaces.
In this work, we investigate protein adsorption from single protein solutions and complex media such as 100% blood serum and plasma onto poly(sulfobetaine methacrylate) (polySBMA)-grafted surfaces via atom transfer radical polymerization (ATRP) at varying film thicknesses. It is interesting to observe that protein adsorption exhibits a minimum at a medium film thickness. Results show that the surface with 62 nm polySBMA brushes presents the best nonfouling character in 100% blood serum and plasma although all of these surfaces are highly resistant to nonspecific protein adsorption from single fibrinogen and lysozyme solutions. Surface resistance to 100% blood serum or plasma is necessary for many applications from blood-contacting devices to drug delivery. This work provides a new in vitro evaluation standard for the application of biomaterials in vivo.
Yang
Wei
W
Department of Chemical Engineering, University of Washington, Box 351750, Seattle, Washington 98195, USA.
Chen
Shengfu
S
Cheng
Gang
G
Vaisocherová
Hana
H
Xue
Hong
H
Li
Wei
W
Zhang
Jinli
J
Jiang
Shaoyi
S
D000327:Adsorption; D001622:Betaine / Q000031:analogs & derivatives* / Q000737:chemistry; D016503:Drug Delivery Systems; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D007477:Ions; D008956:Models, Chemical; D009113:Muramidase / Q000737:chemistry; D010949:Plasma / Q000378:metabolism*; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000737:chemistry*; D044967:Serum / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013499:Surface Properties; D013997:Time Factors
D007477:Ions; D011108:Polymers; D011506:Proteins; D001622:Betaine; C483727:sulfobetaine; D005340:Fibrinogen; D009113:Muramidase