title: Electron capture dissociation, electron detachment dissociation, and collision-induced dissociation of polyamidoamine (PAMAM) dendrimer ions with amino, amidoethanol, and sodium carboxylate surface groups.
abstract: Here, we investigate the effect of the structure (generation) and nature of the surface groups of different polyamidoamine (PAMAM) dendrimers on electron-mediated dissociation, either electron capture dissociation (ECD) or electron detachment dissociation (EDD), and compare the fragmentation with that observed in collision-induced dissociation (CID). ECD and EDD of the PAMAM dendrimers resulted in simple mass spectra, which are straightforward to interpret, whereas CID produced complex mass spectra. The results show that electron-mediated dissociation (ECD and EDD) of PAMAM dendrimers does not depend on the nature of the surface group but tends to occur within the innermost generations. CID of the PAMAM dendrimers showed a strong dependence on the nature of the surface group and occurred mostly in the outer generation. The results demonstrate the potential utility of ECD and EDD as a tool for the structural analysis of PAMAM dendrimers.
authors.affiliation: School of Biosciences, University of Birmingham, Edgbaston, Birmingham, United Kingdom.
authors.forename: Malgorzata A
authors.initials: MA
authors.lastname: Kaczorowska
authors.forename: Helen J
authors.initials: HJ
authors.lastname: Cooper
mesh_terms: D000588:Amines / Q000737:chemistry; D002264:Carboxylic Acids / Q000737:chemistry; D050091:Dendrimers; D004583:Electrons; D000431:Ethanol / Q000737:chemistry; D007477:Ions / Q000737:chemistry; D011073:Polyamines / Q000737:chemistry*; D017670:Sodium Compounds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013499:Surface Properties
chemical_list: D000588:Amines; D002264:Carboxylic Acids; D050091:Dendrimers; D007477:Ions; C104700:PAMAM Starburst; D011073:Polyamines; D017670:Sodium Compounds; D000431:Ethanol