[Supercritical CO2 fluid extraction of Rosmarinus officinalis and capability of extracts eliminate OH radical].
OBJECTIVE
Supercritical CO2 fluid extraction process of antioxidation active components from Rosmarinus officinalis was studied.


METHODS
A new extraction process of components extracted from R. officinalis by supercritical CO2 fluid extraction (SFE-CO2 ) was studied in detail. The capability of that the extract eliminate *OH radical was tested by electron paramagnetic resonance (EPR) technique and spin catch technique.


CONCLUSIONS
With free radical clearance as index, by range and variance analysis, the optimum extraction process conditions were: keeping pressure at 30 MPa and temperature at 75 degrees C for 1 h, in the same time adding alcohol 0.30 mL x g(-1).
Zhang
Chong
C
Material and Chemical Engineering School, Hainan University, Key Lab. of Tropic Biological Resource of Chinese Education Ministry, Haikou 570228, China.
Li
Jia-Cheng
JC
Zhang
De-La
DL
Feng
Yu-Hong
YH
Zhang
Ya-Nan
YN
Chen
Si-Li
SL
D002245:Carbon Dioxide / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000737:chemistry*; D004578:Electron Spin Resonance Spectroscopy; D017665:Hydroxyl Radical / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D027542:Rosmarinus / Q000737:chemistry*
D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; D002245:Carbon Dioxide; D017665:Hydroxyl Radical