title: Rosmarinic acid protects human dopaminergic neuronal cells against hydrogen peroxide-induced apoptosis.
abstract: In this study, we investigated the protective effects of rosmarinic acid (RA) on H(2)O(2)-induced neurotoxicity in human dopaminergic cell line, SH-SY5Y. Results showed that RA significantly attenuated H(2)O(2)-induced reactive oxygen species (ROS) generation and apoptotic cell death. Rosmarinic acid effectively suppressed the up-regulation of Bax and down-regulation of Bcl-2. Furthermore, RA stimulated the antioxidant enzyme heme oxygenase-1 (HO-1). We also demonstrated that the HO-1 induction by RA was associated with the protein kinase A (PKA) and phosphatidylinositiol-3-kinase (PI3K) signaling pathways. These results suggest that RA can protect SH-SY5Y cells under oxidative stress conditions by regulating apoptotic process. Thus, RA should be clinically evaluated for the prevention of neurodegenerative diseases.
authors.affiliation: Department of Biotechnology (BK21 Program), Chosun University, Gwangju 501-759, Republic of Korea.
authors.forename: Hyo Jung
authors.initials: HJ
authors.lastname: Lee
authors.forename: Hong-Suk
authors.initials: HS
authors.lastname: Cho
authors.forename: Euteum
authors.initials: E
authors.lastname: Park
authors.forename: Seung
authors.initials: S
authors.lastname: Kim
authors.forename: Sook-Young
authors.initials: SY
authors.lastname: Lee
authors.forename: Chun-Sung
authors.initials: CS
authors.lastname: Kim
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
authors.forename: Sung-Jun
authors.initials: SJ
authors.lastname: Kim
authors.forename: Hong Sung
authors.initials: HS
authors.lastname: Chun
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002934:Cinnamates / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D053630:Depsides / Q000494:pharmacology*; D004298:Dopamine / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects; D019254:Genes, bcl-2 / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D016877:Oxidants / Q000633:toxicity*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D000975:Antioxidants; D053148:Caspase 3; D002934:Cinnamates; D017868:Cyclic AMP-Dependent Protein Kinases; D053630:Depsides; D004298:Dopamine; D051547:Heme Oxygenase-1; D006861:Hydrogen Peroxide; D016877:Oxidants; D019869:Phosphatidylinositol 3-Kinases; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D048051:p38 Mitogen-Activated Protein Kinases; D000097123:Rosmarinic Acid