Genotypic characterization of Porphyromonas gingivalis isolated from subgingival plaque and blood sample in positive bacteremia subjects with periodontitis.
OBJECTIVE
The objective of this study was to investigate clonal relationship among Porphyromonas gingivalis isolated from subgingival plaque and blood samples in positive transient bacteremia subjects with periodontitis.


METHODS
Unrelated patients with general chronic periodontitis or general aggressive periodontitis requiring scaling and root planing (SRP) were included in the study. Genotyping of each isolate was performed using pulsed field gel electrophoresis technique. Genetic relatedness of strains isolated within an individual or between different patients was determined by dendogram analysis.


RESULTS
Following SRP, from 16 patients, seven patients showed positive P. gingivalis bacteremia and nine were negative. Thirty-two strains were isolated from subgingival plaque and blood samples before and during induced transient bacteremia. The majority of the patients harboured one clonal type. Two patients showed different clones in plaque and blood samples suggesting that more than one clone can be found in subgingival plaque. P. gingivalis isolates from periodontitis patients after transient bacteremia following SRP, revealed a high heterogeneity among isolates.


CONCLUSIONS
In 6/16 subjects the same P. gingivalis isolate was found in the blood and in oral cavity. P. gingivalis heterogeneity suggests no association of a unique clonal type with transient bacteremia.
Pérez-Chaparro
P Juliana
PJ
Equipe de Microbiologie EA 1254, Université Européenne de Bretagne, Université de Rennes 1, Rennes, France.
Gracieux
Patrice
P
Lafaurie
Gloria Inés
GI
Donnio
Pierre-Yves
PY
Bonnaure-Mallet
Martine
M
D000328:Adult; D016470:Bacteremia / Q000382:microbiology*; D055113:Chronic Periodontitis / Q000097:blood / Q000382:microbiology; D002999:Clone Cells / Q000145:classification; D004269:DNA, Bacterial / Q000032:analysis; D003773:Dental Plaque / Q000382:microbiology*; D012534:Dental Scaling; D016521:Electrophoresis, Gel, Pulsed-Field; D005260:Female; D005838:Genotype; D005884:Gingival Hemorrhage / Q000097:blood / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000097:blood / Q000382:microbiology; D010514:Periodontal Pocket / Q000097:blood / Q000382:microbiology; D010518:Periodontitis / Q000097:blood / Q000382:microbiology*; D016966:Porphyromonas gingivalis / Q000145:classification / Q000235:genetics*; D016745:Root Planing
D004269:DNA, Bacterial