title: Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with HbD: a study of 129 samples.
abstract: OBJECTIVE
  To compare ion exchange and boronate affinity chromatography for HbA(1c) estimation in patients with type I and II diabetes having hemoglobin D.
  
  
  METHODS
  Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA(1c) estimation in patients with homozygous and heterozygous D disease.
  
  
  RESULTS
  Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA(1c) estimation.
  
  
  CONCLUSIONS
  The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.
authors.affiliation: Department of Pathology and Microbiology, The Aga Khan University, Karachi, Pakistan. bushra.moiz@aku.edu
authors.forename: Bushra
authors.initials: B
authors.lastname: Moiz
authors.forename: Mashhooda Rasool
authors.initials: MR
authors.lastname: Hashmi
authors.forename: Shahmina
authors.initials: S
authors.lastname: Sadaf
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001786:Blood Glucose; D002846:Chromatography, Affinity / Q000379:methods* / Q000592:standards*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods* / Q000592:standards*; D003920:Diabetes Mellitus / Q000097:blood*; D005215:Fasting / Q000097:blood; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D001786:Blood Glucose; D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; C032001:hemoglobin D