title: [Transcriptional regulation of cytochrome P450 3A4 by four kinds of traditional Chinese medicines].
abstract: OBJECTIVE
  To screen a group of traditional Chinese medicines with effect on pregnane X receptor (PXR)-mediated transcription regulation of P450 3A4 (CYP3A4); and to study whether they can induce the expression of CYP3A4 with a dose, time-dependent manner.
  
  
  METHODS
  Transient cotransfection reporter gene assays were performed with pCI-hPXR-neo, pGL3-CYP3A4-Luc and beta-galactosidase expression plasmid in HepG2 cells.
  
  
  RESULTS
  Rhizoma Curcumae, Atractylodes lancea, A. macrocaphala and Poria cocos could induce transcriptional expression of CYP3A4. In the dose-effect study, 24 h after induction, 500 mg x L(-1) Rhizoma Curcumae, A. lancea, A. macrocaphala and Poria cocos, respectively, could induce the CYP3A4 gene expression with (6.82 +/- 0.09), (6.76 +/- 0.20), (5.49 +/- 0.13) and (4.97 +/- 0.07) folds, as compared with 0.1% DMSO treated cells. In the time-effect study, 500 mg x L(-1) Rhizoma curcumae, A. lancea, A. macrocaphala and Poria cocos for 48 h could induce the CYP3A4 gene expression with (7.74 +/- 0.54), (7.34 +/- 0.10), (5.54 +/- 0.11) and (5.32 +/- 0.18) folds, compared with 0.1% DMSO treated cells.
  
  
  CONCLUSIONS
  Rhizoma Curcumae, A. lancea, A. macrocaphala and Poria cocos could induce the expression of CYP3A4 gene transcription through activating PXR.
authors.affiliation: Institute of Pharmaceutical Biotechnology and Bioengineering, Fuzhou University 350002, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Dong
authors.forename: Jing-Wei
authors.initials: JW
authors.lastname: Shao
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Chen
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.forename: Feng-Ping
authors.initials: FP
authors.lastname: Lin
authors.forename: Yang-Hao
authors.initials: YH
authors.lastname: Guo
mesh_terms: D045744:Cell Line, Tumor; D051544:Cytochrome P-450 CYP3A / Q000235:genetics*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D006801:Humans; D000077297:Pregnane X Receptor; D011987:Receptors, Steroid / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D004365:Drugs, Chinese Herbal; D000077297:Pregnane X Receptor; D011987:Receptors, Steroid; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human