Simultaneous determination of vancomycin and ceftazidime in cerebrospinal fluid in craniotomy patients by high-performance liquid chromatography.
A simple, accurate and rapid method for simultaneous analysis of vancomycin and ceftazidime in cerebrospinal fluid (CSF), utilizing high-performance liquid chromatography (HPLC), has been developed and thoroughly validated to satisfy strict FDA guidelines for bioanalytical methods. Protein precipitation was used as the sample pretreatment method. In order to increase the accuracy, tinidazole was chosen as the internal standard. Separation was achieved on a Diamonsil C18 column (200 mm x 4.6mm I.D., 5 microm) using a mobile phase composed of acetonitrile and acetate buffer (pH 3.5) (8:92, v/v) at room temperature (25 degrees C), and the detection wavelength was 240 nm. All the validation data, such as accuracy, precision, and inter-day repeatability, were within the required limits. The method was applied to determine vancomycin and ceftazidime concentrations in CSF in five craniotomy patients.
Ye
Guangming
G
101st Hospital of PLA, North Xingyuan Road, Wuxi, Jiangsu 214044, PR China. shygm8@hotmail.com
Cai
Xuejian
X
Wang
Biao
B
Zhou
Zhongxian
Z
Yu
Xiaohua
X
Wang
Weibin
W
Zhang
Jiandong
J
Wang
Yuhai
Y
Dong
Jierong
J
Jiang
Yunyun
Y
D000085:Acetates / Q000737:chemistry; D000097:Acetonitriles / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002021:Buffers; D002442:Ceftazidime / Q000032:analysis* / Q000134:cerebrospinal fluid* / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D003399:Craniotomy / Q000379:methods; D004355:Drug Stability; D005615:Freezing; D017408:Guidelines as Topic; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature; D013997:Time Factors; D014011:Tinidazole / Q000737:chemistry; D014640:Vancomycin / Q000032:analysis* / Q000134:cerebrospinal fluid* / Q000737:chemistry
D000085:Acetates; D000097:Acetonitriles; D000900:Anti-Bacterial Agents; D002021:Buffers; D014011:Tinidazole; D014640:Vancomycin; D002442:Ceftazidime; C032159:acetonitrile