High-resolution, in vivo magnetic resonance imaging of Drosophila at 18.8 Tesla.
High resolution MRI of live Drosophila was performed at 18.8 Tesla, with a field of view less than 5 mm, and administration of manganese or gadolinium-based contrast agents. This study demonstrates the feasibility of MR methods for imaging the fruit fly Drosophila with an NMR spectrometer, at a resolution relevant for undertaking future studies of the Drosophila brain and other organs. The fruit fly has long been a principal model organism for elucidating biology and disease, but without capabilities like those of MRI. This feasibility marks progress toward the development of new in vivo research approaches in Drosophila without the requirement for light transparency or destructive assays.
Null
Brian
B
Stanford Genome Technology Center/Department of Biochemistry/Bio-X Program, Stanford, California, United States of America. zenbonz@gmail.com
Liu
Corey W
CW
Hedehus
Maj
M
Conolly
Steven
S
Davis
Ronald W
RW
D000818:Animals; D001921:Brain / Q000473:pathology; D002712:Chlorides / Q000494:pharmacology; D003287:Contrast Media / Q000494:pharmacology; D015509:Developmental Biology / Q000295:instrumentation / Q000379:methods; D004331:Drosophila melanogaster / Q000378:metabolism*; D004867:Equipment Design; D005240:Feasibility Studies; D019786:Gadolinium DTPA / Q000494:pharmacology; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D017895:Manganese Compounds / Q000494:pharmacology; D015203:Reproducibility of Results; D012984:Software
D002712:Chlorides; D003287:Contrast Media; D017895:Manganese Compounds; D019786:Gadolinium DTPA; C025340:manganese chloride