Supplemental sodium butyrate stimulates different gastric cells in weaned pigs.
Sodium butyrate (SB) is used as an acidifier in animal feed. We hypothesized that supplemental SB impacts gastric morphology and function, depending on the period of SB provision. The effect of SB on the oxyntic and pyloric mucosa was studied in 4 groups of 8 pigs, each supplemented with SB either during the suckling period (d 4-28 of age), after weaning (d 29 to 39-40 of age) or both, or never. We assessed the number of parietal cells immunostained for H+/K+-ATPase, gastric endocrine cells immunostained for chromogranin A and somatostatin (SST) in the oxyntic mucosa, and gastrin-secreting cells in the pyloric mucosa. Gastric muscularis and mucosa thickness were measured. Expressions of the H+/K+-ATPase and SST type 2 receptor (SSTR2) genes in the oxyntic mucosa and of the gastrin gene in the pyloric mucosa were evaluated by real-time RT-PCR. SB increased the number of parietal cells per gland regardless of the period of administration (P < 0.05). SB addition after, but not before, weaning increased the number of enteroendocrine and SST-positive cells (P < 0.01) and tended to increase gastrin mRNA (P = 0.09). There was an interaction between the 2 periods of SB treatment for the expression of H/K-ATPase and SSTR2 genes (P < 0.05). Butyrate intake after weaning increased gastric mucosa thickness (P < 0.05) but not muscularis. SB used orally at a low dose affected gastric morphology and function, presumably in relationship with its action on mucosal maturation and differentiation.
Mazzoni
Maurizio
M
Department of Agri-food Protection and Improvement, University of Bologna, 42100 Reggio Emilia, Italy.
Le Gall
Maud
M
De Filippi
Sara
S
Minieri
Laura
L
Trevisi
Paolo
P
Wolinski
Jaroslaw
J
Lalatta-Costerbosa
Giovanna
G
Lallès
Jean-Paul
JP
Guilloteau
Paul
P
Bosi
Paolo
P
D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000833:Animals, Suckling; D020148:Butyric Acid / Q000494:pharmacology*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D019858:Enteroendocrine Cells / Q000187:drug effects / Q000378:metabolism; D005753:Gastric Mucosa / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005755:Gastrins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D017506:H(+)-K(+)-Exchanging ATPase / Q000235:genetics / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000502:physiology*; D014886:Weaning
D005755:Gastrins; D017481:Receptors, Somatostatin; D020148:Butyric Acid; C093733:somatostatin receptor 2; D017506:H(+)-K(+)-Exchanging ATPase