Spatio-temporal differences in the profile of murine brain expression of proinflammatory cytokines and indoleamine 2,3-dioxygenase in response to peripheral lipopolysaccharide administration.
The mechanisms underlying in vivo activation of indoleamine 2,3-dioxygenase (IDO), a tryptophan-catabolizing enzyme that mediates in the brain the induction of depressive-like behavior by peripheral innate immune system stimulation are still poorly understood. By monitoring how cytokines parallel IDO mRNA expression in the brain in response to intraperitoneal lipopolysaccharide injection in mice, we report a time-dependent induction of IDO expression in both the hippocampus and hypothalamus that was associated with a specific structure-dependent expression of proinflammatory cytokines, particularly interferon-gamma. This study suggests that different mechanisms regulate the activation of IDO by lipopolysaccharide in various brain structures.
André
Caroline
C
INRA, UMR 1286 PsyNuGen, Université Victor Ségalen Bordeaux 2, CNRS, UMR 5226, IFR8, F-33076 Bordeaux, France.
O'Connor
Jason C
JC
Kelley
Keith W
KW
Lestage
Jacques
J
Dantzer
Robert
R
Castanon
Nathalie
N
D000818:Animals; D001835:Body Weight / Q000187:drug effects; D016207:Cytokines / Q000097:blood* / Q000235:genetics; D015870:Gene Expression / Q000187:drug effects; D006624:Hippocampus / Q000187:drug effects* / Q000201:enzymology; D007031:Hypothalamus / Q000187:drug effects* / Q000201:enzymology; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase / Q000097:blood* / Q000235:genetics; D007737:Kynurenine / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage*; D008297:Male; D051379:Mice; D012333:RNA, Messenger / Q000378:metabolism; D013997:Time Factors; D014364:Tryptophan / Q000378:metabolism
D016207:Cytokines; D050503:Indoleamine-Pyrrole 2,3,-Dioxygenase; D008070:Lipopolysaccharides; D012333:RNA, Messenger; D007737:Kynurenine; D014364:Tryptophan