[Construction of gtfB/CAT eukaryotic expression plasmid of Streptococcus mutans in mammalian cells].
OBJECTIVE
The study aimed to evaluate the expression of recombinant plasmid pVAX1- gtfB/CAT in mammalian COS-7 cells.


METHODS
The eukaryotic plasmid carrying encoding gene of gtfB/CAT of Streptococcus mutans was constructed and introduced into COS-7 cells by lipofectamine reagent. The transient protein expression was detected by immunochemistry technique in COS-7 cells.


RESULTS
The positive expression of gefB/CAT was detected in plasma of the cells which were transfected with recombinant plasmid pVAX1- gtfB/CAT. The cells which were transfected with pVAX1 were negative for gtfB/CAT expression.


CONCLUSIONS
GtfB/CAT can be translated and expressed in COS-7 cells after transfected with recombinant plasmid pVAX1- gtfB/CAT. The expressed protein is located in the plasma and the protein is able to combine with anti- gtfB/CAT antibody. The expressed protein has the antigenicity and recombinant plasmid pVAX1- gtfB/CAT is a candidate vaccine.Supported by Key Research Project of Bureau of Education of Guizhou Province (Grant No.2004119).
Qu
Yun-Peng
YP
The Affiliated Stomatology Hospital, Wenzhou Medical College. Wenzhou 325027, Zhejiang Province, China. quyunpeng618@163.com
Liu
Jian-Guo
JG
Yang
De-Qin
DQ
Ma
Jian-Feng
JF
Chen
Dai-Xiong
DX
Chai
Qiao-Xue
QX
D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005964:Glucosyltransferases; D010957:Plasmids; D013295:Streptococcus mutans / Q000235:genetics*; D014157:Transcription Factors; D014162:Transfection; D019444:Vaccines, DNA
D014157:Transcription Factors; D019444:Vaccines, DNA; D005964:Glucosyltransferases; C113848:glucosyltransferase D