[The influence of transplanted culture of bone marrow stromal cells on reparative osteohistogenesis in parietal bone defect].
Recently the problem connected with transplantation of bone marrow stromal cells for optimization of reparative osteogenesis is very actively studied. However, the objective methods allowing to observe the behavior of transplanted cells in a bone wound and to estimate character of regenerative osteogenesis after cell transplantation are used in an insufficient measure in both experimental and clinical researches. The aim of this study is to clarify the fate of stromal cells in a bone wound and to investigate the influence of bone marrow stromal cells on the process ofposttraumatic osteogenesis after cell transplantation in parietal bone defect. The experiments were carried out on 38 rabbits with artificially made parietal bone defect (diameter 1.0 cmm). The rabbits were divided into three groups: the first group was a control one; the rabbits of the second group were injected autogenic cultivated bone marrow stromal cells (10(6)); the rabbits of the third group were injected with autogenic cultivated bone marrow stromal cells (10(6)) in collagn gel. The methods of light and fluorescent microscopy, histomorphometry and statistical treatment of the data were used to estimate the results. The obtained data showed that transplanted cells were viable at least during 18 days after transplantation and efficiently took part in the reparative process. The transplantation of cultivated bone marrow stromal cells in collagen gel caused 30% increase in the part of bone tissue in the bone regenerate tissue in comparison with control after 120 days.
Deev
R V
RV
Tsupkina
N V
NV
Gololobov
V G
VG
Nikolaenko
N S
NS
Ivanov
D E
DE
Dulaev
A K
AK
Pinaev
G P
GP
D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology*; D016026:Bone Marrow Transplantation; D001861:Bone Regeneration / Q000502:physiology*; D002478:Cells, Cultured; D005260:Female; D008297:Male; D010012:Osteogenesis; D010294:Parietal Bone / Q000502:physiology*; D011817:Rabbits; D017154:Stromal Cells / Q000502:physiology* / Q000637:transplantation; D013997:Time Factors; D014182:Transplantation, Autologous