Microtubule-associated protein 1B light chain (MAP1B-LC1) negatively regulates the activity of tumor suppressor p53 in neuroblastoma cells.
The tumor suppressor and transcription factor p53 is a key modulator of cellular stress responses and can trigger apoptosis in many cell types, including neurons. In this study, we have shown that the Microtubule-Associated Protein 1B (MAP1B) light chain can interact with the tumor suppressor p53. We also demonstrate that both p53 and the MAP1B light chain (MAP1B-LC1) alter their localization from the cytoplasm to the nucleus when neuroblastoma cells, SH-SY5Y, are treated with doxorubicin. Additionally, we demonstrate that the MAP1B-LC1 negatively regulates p53-dependent transcriptional activity of a reporter construct driven by the p21 promoter. Consequently, MAP1B-LC1 binds to p53 and this interaction leads to the inhibition of doxorubicin-induced apoptosis in SH-SY5Y cells.
Lee
So-Youn
SY
Laboratory of Molecular Biology, Department of Life Science, College of Natural Sciences, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Ku, Seoul, Republic of Korea.
Kim
Jung-Woong
JW
Jeong
Mi-Hee
MH
An
Joo-Hee
JH
Jang
Sang-Min
SM
Song
Ki-Hyun
KH
Choi
Kyung-Hee
KH
D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis; D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000378:metabolism; D003593:Cytoplasm / Q000378:metabolism; D004317:Doxorubicin / Q000494:pharmacology*; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008869:Microtubule-Associated Proteins / Q000378:metabolism*; D009447:Neuroblastoma / Q000235:genetics / Q000378:metabolism*; D017434:Protein Structure, Tertiary; D021381:Protein Transport; D014158:Transcription, Genetic; D015533:Transcriptional Activation; D016159:Tumor Suppressor Protein p53 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
D000903:Antibiotics, Antineoplastic; C475491:MAP1B protein, human; D008869:Microtubule-Associated Proteins; D016159:Tumor Suppressor Protein p53; D004317:Doxorubicin; D008156:Luciferases