Effect of oil-in-water lipid emulsions prepared with fish oil or soybean oil on the growth of MCF-7 cells and HepG2 cells.
The growth of human breast cancer-derived MCF-7 cells was affected by oil-in-water lipid emulsions prepared with fish oil (FO) rich in n-3 fatty acids (FAs) and egg-yolk phosphatides (EYP) (FO-emulsions), but not by lipid emulsions prepared with soybean oil (SO) and EYP (SO-emulsions). On the other hand, the growth of human hepatocarcinoma HepG2 cells was affected by neither SO-emulsions nor FO-emulsions. The growth inhibition of MCF-7 cells in the presence of FO-emulsions was not affected by trolox, but was inhibited by alpha-lipoic acid, and was even potentiated by ebselen, which works as an antioxidant as well as a lipoxygenase inhibitor. Since prostaglandin E(3), generated from n-3 FAs by cyclooxygenases, has a suppressive effect on tumour cell growth, and increases when lipoxygenases are inhibited, these findings suggest that lipid emulsions incorporating triglycerides of n-3 FAs might be effective in suppressing the growth of MCF-7 cells, possibly via oxidative stress and through eicosanoid production with anti-proliferating activity against cancer cells.
Ueda
Kumiko
K
Department of Pharmaceutics, Kobe Pharmaceutical University, 4-19-1 Motoyamakita-machi, Higashinada-ku, Kobe 658-8558, Japan. ueda@kobepharma-u.ac.jp
Asai
Yukiko
Y
Yoshimura
Yoshimizu
Y
Iwakawa
Seigo
S
D000975:Antioxidants / Q000494:pharmacology; D001943:Breast Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D015777:Eicosanoids / Q000378:metabolism; D004655:Emulsions; D004852:Epoxy Compounds / Q000378:metabolism; D005260:Female; D005395:Fish Oils / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology*; D018384:Oxidative Stress / Q000187:drug effects; D010316:Particle Size; D013024:Soybean Oil / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D013997:Time Factors; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000378:metabolism; D014867:Water / Q000737:chemistry*
D000975:Antioxidants; D015777:Eicosanoids; D004655:Emulsions; D004852:Epoxy Compounds; D005395:Fish Oils; D014867:Water; D024482:Vitamin K 2; C030814:menatetrenone; D013024:Soybean Oil