Genotypic screening of atypical Staphylococcus aureus strains isolated from clinical samples for presence of selected adhesin genes.
OBJECTIVE
The aim of this study was to screen Staphylococcus aureus negative for production of coagulase or clumping factor and for presence of selected adhesin genes.


METHODS
Sixty coagulase-negative and 20 clumping factor-negative S. aureus strains were studied. Detection of methicillin resistance was performed using the agar screen technique with 6 mg/L of oxacillin and was confirmed by amplification mecA gene. The presence of bone binding protein (bbp), collagen binding protein (cna), fibronectin A binding protein (fnbA), fibronectin B binding protein (fnbB) and clumping factor A (clfA) genes was detected by multiplex PCR.


RESULTS
Almost all (98%) of the strains were positive for clfA gene. There were fnbA and fnibB in 85%, cna in 54% and bbp in 5% of strains found. No correlation between presence of the particular genes and clinical samples was observed. The prevalence of fnbA, fnbB and cna was statistically higher in coagulase-negative than in clumping factor-negative strains (89, 89, 66 and 70, 70, 15%, respectively). Similarly, all of these genes were more often observed in MRSA than in MSSA atypical strains. The cna was detected only in coagulase-negative MRSA.
Wiśniewska
K
K
Department of Medical Microbiology, Medical University of Gdańsk, 38, Do Studzienki Street, 80-227 Gdańsk, Poland. kwis@amg.gda.pl
Garbacz
K
K
Piechowicz
L
L
D018829:Adhesins, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D002352:Carrier Proteins / Q000235:genetics; D003030:Coagulase / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification; D010068:Oxacillin / Q000494:pharmacology; D011044:Poland / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003030:Coagulase; C048165:adhesin, Staphylococcus aureus; C405490:bone sialoprotein-binding protein, Staphylococcus aureus; C078635:fibronectin-binding proteins, bacterial; D010068:Oxacillin