title: Identification of residues in the Cmu4 domain of polymeric IgM essential for interaction with Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1).
abstract: The binding of nonspecific human IgM to the surface of infected erythrocytes is important in rosetting, a major virulence factor in the pathogenesis of severe malaria due to Plasmodium falciparum, and IgM binding has also been implicated in placental malaria. Herein we have identified the IgM-binding parasite ligand from a virulent P. falciparum strain as PfEMP1 (TM284var1 variant), and localized the region within this PfEMP1 variant that binds IgM (DBL4beta domain). We have used this parasite IgM-binding protein to investigate the interaction with human IgM. Interaction studies with domain-swapped Abs, IgM mutants, and anti-IgM mAbs showed that PfEMP1 binds to the Fc portion of the human IgM H chain and requires the IgM Cmu4 domain. Polymerization of IgM was shown to be crucial for the interaction because PfEMP1 binding did not occur with mutant monomeric IgM molecules. These results with PfEMP1 protein have physiological relevance because infected erythrocytes from strain TM284 and four other IgM-binding P. falciparum strains showed analogous results to those seen with the DBL4beta domain. Detailed investigation of the PfEMP1 binding site on IgM showed that some of the critical amino acids in the IgM Cmu4 domain are equivalent to those regions of IgG and IgA recognized by Fc-binding proteins from bacteria, suggesting that this region of Ig molecules may be of major functional significance in host-microbe interactions. We have therefore shown that PfEMP1 is an Fc-binding protein of malaria parasites specific for polymeric human IgM, and that it shows functional similarities with Fc-binding proteins from pathogenic bacteria.
authors.affiliation: Institute of Genetics, Queens Medical Centre, University of Nottingham, Nottingham, England, United Kingdom.
authors.forename: Ashfaq
authors.initials: A
authors.lastname: Ghumra
authors.forename: Jean-Philippe
authors.initials: JP
authors.lastname: Semblat
authors.forename: Richard S
authors.initials: RS
authors.lastname: McIntosh
authors.forename: Ahmed
authors.initials: A
authors.lastname: Raza
authors.forename: Ingunn B
authors.initials: IB
authors.lastname: Rasmussen
authors.forename: Ranveig
authors.initials: R
authors.lastname: Braathen
authors.forename: Finn-Eirik
authors.initials: FE
authors.lastname: Johansen
authors.forename: Inger
authors.initials: I
authors.lastname: Sandlie
authors.forename: Patricia K
authors.initials: PK
authors.lastname: Mongini
authors.forename: J Alexandra
authors.initials: JA
authors.lastname: Rowe
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Pleass
mesh_terms: D020816:Amino Acid Motifs; D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D019556:COS Cells; D002522:Chlorocebus aethiops; D004912:Erythrocytes / Q000276:immunology / Q000378:metabolism; D006801:Humans; D007141:Immunoglobulin Fc Fragments / Q000276:immunology; D007075:Immunoglobulin M / Q000737:chemistry / Q000276:immunology*; D008024:Ligands; D008958:Models, Molecular; D008969:Molecular Sequence Data; D010963:Plasmodium falciparum / Q000276:immunology*; D011485:Protein Binding; D020836:Protein Structure, Quaternary; D015800:Protozoan Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D016415:Sequence Alignment; D017386:Sequence Homology, Amino Acid; D012995:Solubility
chemical_list: D007141:Immunoglobulin Fc Fragments; D007075:Immunoglobulin M; D008024:Ligands; D015800:Protozoan Proteins; C085731:erythrocyte membrane protein 1, Plasmodium falciparum; C024242:polymeric IgM