On-site manipulation of single chromosomal DNA molecules by using optically driven microstructures.
We report a novel method for manipulation of single giant DNA molecules under a video microscope. Using optically driven microstructures, we manipulated chromosomal DNA of length in the order of millimetres, extended by electroosmotic flow without DNA breakage in aqueous solution: we picked up DNA, using microfabricated hooks and wound it around microfabricated bobbins.
Terao
Kyohei
K
Department of Micro Engineering, Kyoto University, Kyoto, 606-8501, Japan. k.terao@kt5.ecs.kyoto-u.ac.jp
Washizu
Masao
M
Oana
Hidehiro
H
D002875:Chromosomes / Q000648:ultrastructure*; D004271:DNA, Fungal / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation* / Q000379:methods*; D012568:Schizosaccharomyces / Q000648:ultrastructure
D004271:DNA, Fungal