Secretome analysis of Phanerochaete chrysosporium strain CIRM-BRFM41 grown on softwood.
Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidase hypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions. Extracellular proteins from both cultures were analyzed by bidimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. A total of 37 spots were identified. The secretome in liquid synthetic medium comprised mainly peroxidases, while several wood-degrading enzymes and enzymes involved in fungal metabolism were detected in biopulping cultures on softwood. This prompted an analysis of the impact of secretome modulation in the presence of softwood chips. Biotreated wood was submitted to kraft cooking and chemical bleaching using chlorine dioxide. The fungal pre-treatment led to a significant increase in pulp yield and a better bleachability of the pulp. This bleachability improvement could be explained by the production of specific lignocellulose-degrading enzymes.
Ravalason
Holy
H
INRA, UMR1163, Biotechnologie des Champignons Filamenteux, 13000, Marseille, France. holy.ravalason@esil.univmed.fr
Jan
Gwénaël
G
Mollé
Daniel
D
Pasco
Maryvonne
M
Coutinho
Pedro M
PM
Lapierre
Catherine
C
Pollet
Brigitte
B
Bertaud
Frédérique
F
Petit-Conil
Michel
M
Grisel
Sacha
S
Sigoillot
Jean-Claude
JC
Asther
Marcel
M
Herpoël-Gimbert
Isabelle
I
D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005110:Extracellular Space / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007218:Industrial Microbiology; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D040901:Proteomics; D014934:Wood / Q000737:chemistry / Q000378:metabolism*
D005656:Fungal Proteins; D008031:Lignin