A pilot study of colonic B cell pattern in irritable bowel syndrome.
OBJECTIVE
Low-grade gastrointestinal inflammation has been reported in patients with irritable bowel syndrome (IBS). However, the colonic B-cell pattern has not been investigated in these patients. Therefore, the aim of this pilot study was to investigate the distribution and isotype of immunoglobulin-producing B cells in the colonic mucosa of IBS patients.


METHODS
Patients with IBS (n=12) fulfilling the Rome II criteria were compared with controls (n=11). Immunohistochemical staining of biopsies from the sigmoid and ascending colon was performed.


RESULTS
The number of IgA(+) B cells in the ascending colon was lower in IBS patients than in controls (p=0.039). Furthermore, unlike controls, IBS patients had a reduction of IgA(+) B cells in the ascending colon relative to the sigmoid colon (p=0.04). Neither the IgG(+), nor the IgM(+) colonic B-cell numbers differed between IBS patients and controls. Very few colonic IgE(+) cells were detected and there was no difference between the two subject groups.


CONCLUSIONS
The reduced number of colonic IgA(+) B cells in IBS patients suggests that the disorder may be associated with a modified gut immune defence. Whether this phenomenon is causally related to symptoms remains unknown and merits further investigation in a larger group of patients.
Forshammar
Johan
J
Department of Medicine, Sahlgrenska Academy of Göteborg University, Sweden.
Isaksson
Stefan
S
Strid
Hans
H
Stotzer
Per-Ove
PO
Sjövall
Henrik
H
Simrén
Magnus
M
Ohman
Lena
L
D000328:Adult; D001402:B-Lymphocytes / Q000276:immunology*; D003106:Colon / Q000276:immunology*; D005260:Female; D006801:Humans; D007070:Immunoglobulin A; D007073:Immunoglobulin E; D007075:Immunoglobulin M; D007249:Inflammation / Q000276:immunology*; D043183:Irritable Bowel Syndrome / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects
D007070:Immunoglobulin A; D007075:Immunoglobulin M; D007073:Immunoglobulin E