Analysis of urinary aromatic acids by liquid chromatography tandem mass spectrometry.
The separation and detection of 11 urinary aromatic acids was developed using HPLC-MS/MS. The method features a simple sample preparation involving a single-step dilution with internal standard and a rapid 8 min chromatographic separation. The accuracy was evaluated by the recovery of known spikes between 87 and 110%. Inter- and intra-assay precision (CV) was below 11% in all cases and the analytes were observed to be stable for up to 8 weeks when stored at -20 degrees C. The method was validated based upon linearity, accuracy, precision and stability and was used to establish reference intervals for children and adults.
Crow
Brian
B
Metametrix Clinical Laboratory, 3425 Corporate Way, Duluth, GA 30096, USA. bcrow@metametrix.com
Bishop
Michael
M
Paliakov
Ekaterina
E
Norton
Dean
D
George
Joe
J
Bralley
J Alexander
JA
D000147:Acids, Heterocyclic / Q000652:urine*; D000328:Adult; D002109:Caffeic Acids / Q000652:urine; D002264:Carboxylic Acids / Q000652:urine*; D002648:Child; D002853:Chromatography, Liquid / Q000379:methods*; D006626:Hippurates / Q000652:urine; D006719:Homovanillic Acid / Q000652:urine; D006801:Humans; D007736:Kynurenic Acid / Q000652:urine; D010226:Parabens / Q000032:analysis; D010648:Phenylacetates / Q000652:urine; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014642:Vanilmandelic Acid / Q000652:urine; D014977:Xanthurenates / Q000652:urine
D000147:Acids, Heterocyclic; D002109:Caffeic Acids; D002264:Carboxylic Acids; D006626:Hippurates; D010226:Parabens; D010648:Phenylacetates; D014977:Xanthurenates; C000995:3,4-dihydroxyphenylpropionic acid; C008070:4-hydroxyphenylacetic acid; D014642:Vanilmandelic Acid; C028330:xanthurenic acid; C025136:phenylacetic acid; D007736:Kynurenic Acid; C038193:4-hydroxybenzoic acid; C030514:hippuric acid; D006719:Homovanillic Acid