The lysogenic cycle of the filamentous phage Cflt from Xanthomonas campestris pv. citri.
A phage, Cflt, forming turbid plaques, was isolated from Xanthomonas campestris pv. citri. After infection, infected sensitive cells become immune to Cflt and produce very few phages. These properties were genetically rather stable. The phage was purified and shown to be filamentous with a size of 1157 +/- 73 nm. The genome size is about 7.62 kb. The phage does not affect the growth of host bacteria. Under natural cultivation conditions Cflt-lysogenized cells could be induced spontaneously to give high phage yields, or cured to give phage-free cells. The integration of Cflt DNA into host DNA was proved by Southern blot hybridization. The lysogenic phage was genetically stable in log phase cells and persisted in stationary phase cells through many cell generations in the absence of extracellular phage reinfection.
Kuo
T T
TT
Institute of Botany, Academia Sinica, Taipei, Republic of China.
Lin
Y H
YH
Huang
C M
CM
Chang
S F
SF
Dai
H
H
Feng
T Y
TY