title: Mixed self-assembly of polydiacetylenes for highly specific and sensitive strip biosensors.
abstract: Polydiacetylenes (PDAs), which possess unique properties that allow them to change color in response to environmental changes such as variations in pH, temperature, and molecular binding, have been widely investigated as signal transducers in biosensor applications. Most PDA-based sensors reported to date have been evaluated largely on the basis of their ability to detect purified samples, however, and their specificity has rarely been tested. In this study, novel PDAs fabricated on polyvinylidene fluoride (PVDF) strips by photoreaction of composite diacetylene self-assemblies were developed as biosensors, and nonspecific binding to off-target biomolecules was assessed. A mixed PDA surface containing biotin and ethanolamide bound the target, i.e., streptavidin, more specifically than did biotin alone. The optimized PDA biosensor exhibited approximately 2850-fold higher selectivity for streptavidin relative to bovine serum albumin controls. A PDA biosensor that was further prepared showed distinctive signals for the urine of diabetic patients compared to urine samples from healthy/non-diabetic person due to the concentration of microalbuminuria. To our knowledge, this is the first strip-type biosensor fabricated with PDAs and the first PDA-based biosensor that can effectively overcome the problem of nonspecific binding.
authors.affiliation: BioNanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Yuseong, Daejeon, Republic of Korea.
authors.forename: Hyun Kyu
authors.initials: HK
authors.lastname: Park
authors.forename: Sang J
authors.initials: SJ
authors.lastname: Chung
authors.forename: Hyun Gyu
authors.initials: HG
authors.lastname: Park
authors.forename: Jae-Hyoung
authors.initials: JH
authors.lastname: Cho
authors.forename: Moonil
authors.initials: M
authors.lastname: Kim
authors.forename: Bong Hyun
authors.initials: BH
authors.lastname: Chung
mesh_terms: D000419:Albuminuria / Q000175:diagnosis; D015374:Biosensing Techniques / Q000379:methods*; D001710:Biotin / Q000737:chemistry; D003928:Diabetic Nephropathies / Q000175:diagnosis; D006863:Hydrogen-Ion Concentration; D000078789:Polyacetylene Polymer; D011108:Polymers / Q000737:chemistry*; D053279:Polyynes / Q000737:chemistry*; D011934:Reagent Strips; D012680:Sensitivity and Specificity
chemical_list: D000078789:Polyacetylene Polymer; D011108:Polymers; D011934:Reagent Strips; D053279:Polyynes; C082361:polydiacetylene; D001710:Biotin