Evidence for tyrosinase as a beta1,6 branch containing glycoprotein: substrate of GnT-V.
Tyrosinase is a rate-limiting enzyme in mammalian melanogenesis, and is known as a glycoprotein. Post-translational processing of mammalian tyrosinase is required for its folding, sorting, and for enzymatic activity. Here we show for the first time that the mammalian tyrosinase has beta1,6-branched N-glycan structure that can be recognized by binding with specific lectin Leukoagglutinating phytohematoagglutinin (L-PHA). Further, this specific glycoconjugate structure has been shown to have a function relationship in melanin synthesis.
Chakraborty
Debjit
D
Department of Psychiatry, Calcutta Medical College, Kolkata, West Bengal, 700073 India.
Chakraborty
Ashok K
AK
D000818:Animals; D002240:Carbohydrate Sequence; D045744:Cell Line, Tumor; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D008543:Melanins / Q000096:biosynthesis; D051379:Mice; D014442:Monophenol Monooxygenase / Q000737:chemistry / Q000378:metabolism*; D017351:N-Acetylglucosaminyltransferases / Q000737:chemistry / Q000378:metabolism*; D010835:Phytohemagglutinins / Q000737:chemistry; D013329:Structure-Activity Relationship; D013379:Substrate Specificity
D006023:Glycoproteins; D008543:Melanins; D010835:Phytohemagglutinins; D014442:Monophenol Monooxygenase; D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase