Stable repeated-batch production of recombinant dye-decolorizing peroxidase (rDyP) from Aspergillus oryzae.
Recombinant Aspergillus oryzae expressing a dye-decolorizing peroxidase gene (dyp) was cultivated for repeated-batch production of recombinant dye-decolorizing peroxidase (rDyP) using maltose as a carbon source. High-level rDyP activity in limitation of carbon and nitrogen sources was maintained stably for 26 cycles of repeated 1-d batches of A. oryzae pellets without any additional pH control. Cultures maintained at 4 degrees C for 20 d resumed rDyP production following a single day of incubation. One liter filtrated crude rDyP containing 4600 U rDyP decolorized 5.07 g RBBR at the apparent decolorization rate of 17.7 mg l(-1) min(-1).
Shakeri
Mozaffar
M
Chemical Resources Laboratory, Tokyo Institute of Technology, Midori-ku, Yokohama, Japan.
Sugano
Yasushi
Y
Shoda
Makoto
M
D001236:Aspergillus oryzae / Q000201:enzymology*; D018929:Cell Culture Techniques / Q000379:methods*; D003116:Color; D004396:Coloring Agents / Q000737:chemistry* / Q000302:isolation & purification*; D004789:Enzyme Activation; D004795:Enzyme Stability; D007220:Industrial Waste / Q000517:prevention & control; D009195:Peroxidase / Q000302:isolation & purification* / Q000378:metabolism*
D004396:Coloring Agents; D007220:Industrial Waste; D009195:Peroxidase