Elevated neuroimmune biomarkers in sweat patches and plasma of premenopausal women with major depressive disorder in remission: the POWER study.
BACKGROUND
Major depressive disorder (MDD) is inconsistently associated with elevations in proinflammatory cytokines and neuropeptides. We used a skin sweat patch, recently validated in healthy control subjects, and recycling immunoaffinity chromatography to measure neuroimmune biomarkers in patients with MDD mostly in remission.


METHODS
We collected blood at 8:00 am and applied skin sweat patches for 24 hours in 21- to 45-year-old premenopausal women (n = 19) with MDD (17/19 in remission) and age-matched healthy controls (n = 17) participating in the POWER (Premenopausal, Osteopenia/Osteoporosis, Women, Alendronate, Depression) Study.


RESULTS
Proinflammatory cytokines, neuropeptide Y, substance P, and calcitonin-gene-related peptide were significantly higher and vasoactive intestinal peptide, a marker of parasympathetic activity, was significantly lower in patients compared to controls, and depressive symptomatology strongly correlated with biomarker levels. All analytes were strongly correlated in the skin sweat patch and plasma in patients (r = .73 to .99; p < .0004).


CONCLUSIONS
The skin sweat patch allows detection of disrupted patterns of proinflammatory cytokines and neuropeptides in women with MDD in clinical remission, which could predispose to medical consequences such as cardiovascular disease, osteoporosis, and diabetes. This method permits measurement of cytokines in ambulatory settings where blood collection is not feasible.
Cizza
Giovanni
G
Clinical Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20852, USA.
Marques
Andrea H
AH
Eskandari
Farideh
F
Christie
Israel C
IC
Torvik
Sara
S
Silverman
Marni N
MN
Phillips
Terry M
TM
Sternberg
Esther M
EM
D000328:Adult; D015415:Biomarkers / Q000097:blood*; D015740:Calcitonin Gene-Related Peptide / Q000097:blood; D016022:Case-Control Studies; D002395:Catecholamines / Q000097:blood; D002846:Chromatography, Affinity / Q000379:methods; D016207:Cytokines / Q000097:blood*; D003865:Major Depressive Disorder / Q000097:blood / Q000150:complications*; D005260:Female; D006801:Humans; D006854:Hydrocortisone / Q000097:blood; D008137:Longitudinal Studies; D009478:Neuropeptide Y / Q000097:blood; D017697:Premenopause / Q000523:psychology*; D013373:Substance P / Q000097:blood; D013542:Sweat / Q000276:immunology*; D055815:Young Adult
D015415:Biomarkers; D002395:Catecholamines; D016207:Cytokines; D009478:Neuropeptide Y; D013373:Substance P; D015740:Calcitonin Gene-Related Peptide; D006854:Hydrocortisone