title: Characterization of monoclonal antibodies against avian reovirus strain S1133.
abstract: Monoclonal antibodies (MAbs) were produced against a vaccinal S1133 strain of avian reovirus. Characterization of six MAbs in Western blotting, radioimmunoprecipitation and gold immunoelectron microscopy revealed that the MAbs were specific to the outer capsid proteins, mu2/mu2c, sigma2 and sigma3. Two of three MAbs, directed against sigma2 protein, neutralized the virus infectivity in a broadly specific manner, whereas the third had no neutralizing activity. The only MAb which reacted with sigma3 protein showed a type-specific neutralizing activity. Two MAbs recognizing the mu2/mu2c proteins failed to neutralize the virus infectivity.
authors.affiliation: Section de Virologie, Faculte de Medecine Veterinaire, Universite de Montreal, St-Hyacinthe, Quebec, Canada.
authors.forename: M R
authors.initials: MR
authors.lastname: Shapouri
authors.forename: D
authors.initials: D
authors.lastname: Frenette
authors.forename: R
authors.initials: R
authors.lastname: Larochelle
authors.forename: M
authors.initials: M
authors.lastname: Arella
authors.forename: A
authors.initials: A
authors.lastname: Silim