title: Modulation of antioxidant and detoxification responses mediated by lipoic acid in the fish Corydoras paleatus (Callychthyidae).
abstract: Lipoic acid (LA) has been reported as a potential therapeutic agent due its antioxidants proprieties. It was considered its effect in different organs (gills, brain, muscle and liver) of the fish Corydoras paleatus (Callychthyidae). LA (70 mg/kg of body mass) was added to a commercial fish diet, organisms being fed daily (1% body weight). Sixty animals (mean mass: 2.37+/-0.09 g) were placed randomly in aquariums and received (+LA) or not (-LA) lipoic acid enriched diet during four weeks. After, fish were killed and the brain, muscle, gills and liver were dissected. LA treatment reduced significantly (p<0.05) reactive oxygen species concentration in brain and increased (p<0.05) glutamate-cysteine ligase activity in brain and liver of the same experimental group. LA fed organisms showed higher (p<0.05) brain glutathione-S-transferase activity, indicating that LA improves the detoxification and antioxidant capacity face components that waste glutathione in phase II reactions. A conspicuous reduction of protein oxidation was observed in muscle and liver of +LA organisms, indicating that the treatment was also effective in reducing oxidative stress parameters.
authors.affiliation: Departamento de Ciências Fisiológicas, Universidade Federal do Rio Grande - FURG, Rio Grande, RS, Brazil. josemmonserrat@pesquisador.cnpq.br
authors.forename: José Maria
authors.initials: JM
authors.lastname: Monserrat
authors.forename: Juliane Ventura
authors.initials: JV
authors.lastname: Lima
authors.forename: Josencler Luis Ribas
authors.initials: JL
authors.lastname: Ferreira
authors.forename: Daiane
authors.initials: D
authors.lastname: Acosta
authors.forename: Márcia Longaray
authors.initials: ML
authors.lastname: Garcia
authors.forename: Patricia Baptista
authors.initials: PB
authors.lastname: Ramos
authors.forename: Tarsila Barros
authors.initials: TB
authors.lastname: Moraes
authors.forename: Luciane Cougo
authors.initials: LC
authors.lastname: Dos Santos
authors.forename: Lílian Lund
authors.initials: LL
authors.lastname: Amado
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D001921:Brain / Q000187:drug effects / Q000378:metabolism; D002397:Catfishes / Q000378:metabolism*; D005880:Gills / Q000187:drug effects / Q000378:metabolism; D005721:Glutamate-Cysteine Ligase / Q000378:metabolism; D005980:Glutathione Reductase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D008063:Thioctic Acid / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D008063:Thioctic Acid; D005980:Glutathione Reductase; D005982:Glutathione Transferase; D005721:Glutamate-Cysteine Ligase