Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.
Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21(st) century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.
Deepak
Sa
S
Department of Studies in Applied Botany and Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, India.
Kottapalli
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K
Rakwal
R
R
Oros
G
G
Rangappa
Ks
K
Iwahashi
H
H
Masuo
Y
Y
Agrawal
Gk
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