title: Corneal recovery after lasik for high myopia: a 2-year prospective confocal microscopic study.
abstract: OBJECTIVE
  To quantify human corneal recovery after moderate to high myopic laser in situ keratomileusis (LASIK) in a 2-year prospective follow-up study.
  
  
  METHODS
  Fifteen eyes of 15 patients (mean refraction -10.1 (SD 2.4) D) were examined preoperatively and postoperatively at day 1, 5 days, 2 weeks, 1, 3 and 6 months and 2 years. Biomicroscopy, visual acuity and refraction were examined prior to imaging studies. An in vivo tandem scanning confocal microscope was used to obtain images from the central cornea. Subbasal nerve density was measured as the total length of nerve trunks in confocal image per mm2. Keratocyte density was calculated manually from stromal sublayers. The thickness of the altered keratocyte zone was measured on both sides of the LASIK interface.
  
  
  RESULTS
  At the end of the follow-up, all patients had a 20/20 BCVA, and nine of 15 patients were within +/-0.5 D of the intended correction. The total corneal thickness remained unaltered, but epithelial hyperplasia was seen at 2 years. Keratocyte density in the anterior stroma and posterior to the flap interface showed a slight decrease during the follow-up. Subbasal nerve density decreased 82% in 5 days after LASIK. A gradual increase was observed from 2 weeks postoperatively, but even 2 years after the operation the nerve density was only 64% from the preoperative values.
  
  
  CONCLUSIONS
  Subbasal nerve fibre density shows a gradual recovery throughout the follow-up. However, only three subjects showed totally regenerated subbasal nerve fibres at 2 years. This may correlate with the observed decrease in the density of the most anterior keratocytes. Corneal remodelling seemed to continue for at least 2 years.
authors.affiliation: Helsinki University Central Hospital, Department of Ophthalmology, Helsinki, Finland. jukka.moilanen@hus.fi
authors.forename: J A O
authors.initials: JA
authors.lastname: Moilanen
authors.forename: J M
authors.initials: JM
authors.lastname: Holopainen
authors.forename: M H
authors.initials: MH
authors.lastname: Vesaluoma
authors.forename: T M T
authors.initials: TM
authors.lastname: Tervo
mesh_terms: D000328:Adult; D003319:Corneal Stroma / Q000294:innervation / Q000601:surgery*; D005260:Female; D005500:Follow-Up Studies; D006801:Humans; D020731:Keratomileusis, Laser In Situ / Q000379:methods*; D008297:Male; D018613:Microscopy, Confocal; D008875:Middle Aged; D009216:Myopia / Q000534:rehabilitation / Q000601:surgery*; D009412:Nerve Fibers / Q000502:physiology; D009416:Nerve Regeneration / Q000502:physiology; D011184:Postoperative Period; D011446:Prospective Studies; D020127:Recovery of Function; D016896:Treatment Outcome; D014792:Visual Acuity / Q000502:physiology; D014945:Wound Healing / Q000502:physiology