title: Characterization of the glycine betaine biosynthetic genes in the moderately halophilic bacterium Halobacillus dabanensis D-8(T).
abstract: The gene cluster involved in the choline-glycine betaine conversion pathway was cloned from chromosomal DNA of the Gram-positive moderate halophile Halobacillus dabanensis D-8(T). Nucleotide sequence analysis revealed four genes, designated gbsT, gbsI, gbsA, and gbsB, which are clustered in a 5.1-kb fragment. After heterologous expression of gbsAB in the Escherichia coli mutant strain PD141, the transformed cells were able to grow in a selective M63 medium containing 0.7 M NaCl and 1 mM choline, in contrast to the mutant strain. Glycine betaine biosynthesis was restored and its accumulation was confirmed by using (13)C nuclear magnetic resonance spectroscopy.
authors.affiliation: Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, and Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, No. 2 Yuanmingyuan West Road, Beijing, 100193, China.
authors.forename: Zhi Jing
authors.initials: ZJ
authors.lastname: Gu
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Daniel
authors.initials: D
authors.lastname: Le Rudulier
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.forename: Su Sheng
authors.initials: SS
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D001406:Bacillaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001622:Betaine / Q000378:metabolism*; D002794:Choline / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism* / Q000494:pharmacology; D014882:Water-Electrolyte Balance
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D001622:Betaine; D012965:Sodium Chloride; D002794:Choline