Effects of crop development on the emission of volatiles in leaves of Lycopersicon esculentum and its inhibitory activity to Botrytis cinerea and Fusarium oxysporum.
Volatiles emitted from the leaves of Lycopersicon esculentum at the two-, ten-leaf and anthesis periods were collected by a gas absorbing method and analyzed by gas chromatography (GC)-mass spectrometry. In total, 33 compounds of volatiles emitted from three developmental stage plants were separated and identified, and quantitatively analyzed by the internal standard addition method. All of the samples of volatile were found to be rich in monoterpenes and sesquiterpenes. beta-phellandrene and caryophyllene predominated in the volatiles of the leaves of plants at the two- and ten-leaf stages. Furthermore, (E)-2-hexenal were the dominant components in the volatiles emitted from anthesis plants. The results of volatiles analyzed show that the compositions varied depending on the developmental stages. The volatiles emitted from crushed tomato leaves of plants at the anthesis stage had the most strongly inhibitory activity against the spore germination and hyphal growth of Botrytis cinerea and Fusarium oxysporum, followed by ten- and two-leaf plants. However, the activity of volatiles, emitted from the leaves of plants at the two-leaf stage, in inhibiting F. oxysporum was greater than B. cinerea.
Zhang
Peng-Ying
PY
College of Life Sciences, Shandong University, Jinan 250100, China.
Chen
Kao-Shan
KS
He
Pei-Qing
PQ
Liu
Sheng-Hao
SH
Jiang
Wan-Feng
WF
D020171:Botrytis / Q000187:drug effects* / Q000502:physiology; D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D005670:Fusarium / Q000187:drug effects* / Q000502:physiology; D008401:Gas Chromatography-Mass Spectrometry; D006591:Hexobarbital / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D025301:Hyphae / Q000187:drug effects / Q000254:growth & development; D018551:Solanum lycopersicum / Q000378:metabolism*; D039821:Monoterpenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D053147:Plant Exudates / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D018515:Plant Leaves / Q000378:metabolism*; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D013172:Spores, Fungal / Q000187:drug effects / Q000502:physiology; D014835:Volatilization
D000081005:Cyclohexane Monoterpenes; D053138:Cyclohexenes; D039821:Monoterpenes; D053147:Plant Exudates; D000081223:Polycyclic Sesquiterpenes; D012717:Sesquiterpenes; C058582:beta-phellandrene; D006591:Hexobarbital; C024714:caryophyllene