Inactivation of Listeria monocytogenes in brine and saline by alternating high-voltage pulsed current.
The inactivating efficiency of alternating high-voltage pulsed (AHVP) current was investigated in brine (20 w/v% NaCl) and saline (0.9 w/v% NaCl) inoculated with 1x 10(7) cells/ml of Listeria monocytogenes. AHVP current at 12 V with 1 pulse completely inactivated L. monocytogenes in brine within 3 ms, while the bacteria in saline were fully inactivated by 10-pulsed electric treatment at 12 V within the same time. Electron microscopic observation demonstrated substantial structural damage of electrically treated L. monocytogenes in brine. These results suggest that AHVP treatment would be effective for the rapid and complete inactivation of L. monocytogenes in brine or saline solution.
Lee
Mi Hee
MH
Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea.
Han
Dong-Wook
DW
Woo
Yeon I
YI
Uzawa
Masakazu
M
Park
Jong-Chul
JC
D004560:Electricity; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development*; D050296:Microbial Viability / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology*
D012965:Sodium Chloride