title: S1P differentially regulates migration of human ovarian cancer and human ovarian surface epithelial cells.
abstract: Epithelial ovarian cancer (EOC) arises from the epithelial layer covering the surface of ovaries and i.p. metastasis is commonly observed at diagnosis. Sphingosine-1-phosphate (S1P), a bioactive lipid signaling molecule, is potentially involved in EOC tumorigenesis. We have found that S1P is elevated in human EOC ascites. We show that physiologically relevant concentrations of S1P stimulate migration and invasion of EOC cells but inhibit migration of human ovarian surface epithelial (HOSE) cells. In addition, S1P inhibits lysophosphatidic acid (LPA)-induced cell migration in HOSE but not in EOC cells. We have provided the first line of evidence that the expression levels of S1P receptor subtypes are not the only determinants for how cells respond to S1P. Although S1P(1) is expressed and functional in HOSE cells, the inhibitory effect mediated by S1P(2) is dominant in those cells. The cellular preexisting stress fibers are also important determinants for the migratory response to S1P. Differential S1P-induced morphology changes are noted in EOC and HOSE cells. Preexisting stress fibers in HOSE cells are further enhanced by S1P treatment, resulting in the negative migratory response to S1P. By contrast, EOC cells lost stress fibers and S1P treatment induces filopodium-like structures at cell edges, which correlates with increased cell motility. In addition, inhibition of the protein kinase C pathway is likely to be involved in the inhibitory effect of S1P on LPA-induced cell migration in HOSE cells. These findings are important for the development of new therapeutics targeting S1P and LPA in EOC.
authors.affiliation: Department of Obstetrics and Gynecology, Indiana University, 975 West Walnut Street IB355A, Indianapolis, IN 46202, USA.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.forename: Zhenwen
authors.initials: Z
authors.lastname: Zhao
authors.forename: Andrea
authors.initials: A
authors.lastname: Caperell-Grant
authors.forename: Gong
authors.initials: G
authors.lastname: Yang
authors.forename: Samuel C
authors.initials: SC
authors.lastname: Mok
authors.forename: Jinsong
authors.initials: J
authors.lastname: Liu
authors.forename: Robert M
authors.initials: RM
authors.lastname: Bigsby
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
mesh_terms: D001201:Ascites / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D048430:Cell Shape / Q000187:drug effects; D004789:Enzyme Activation / Q000187:drug effects; D004847:Epithelial Cells / Q000187:drug effects / Q000201:enzymology / Q000473:pathology*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000037:antagonists & inhibitors; D005260:Female; D006801:Humans; D008246:Lysophospholipids / Q000494:pharmacology*; D020929:Mitogen-Activated Protein Kinase Kinases / Q000037:antagonists & inhibitors; D010051:Ovarian Neoplasms / Q000201:enzymology / Q000473:pathology*; D011493:Protein Kinase C / Q000378:metabolism; D049349:Receptors, Lysosphingolipid / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D013110:Sphingosine / Q000031:analogs & derivatives* / Q000494:pharmacology; D022502:Stress Fibers / Q000187:drug effects / Q000473:pathology
chemical_list: D008246:Lysophospholipids; D049349:Receptors, Lysosphingolipid; C060506:sphingosine 1-phosphate; D011493:Protein Kinase C; D048049:Extracellular Signal-Regulated MAP Kinases; D020929:Mitogen-Activated Protein Kinase Kinases; D013110:Sphingosine; C032881:lysophosphatidic acid