title: [Study of nHACP/CF co-cultured with osteoblasts in vitro].
abstract: OBJECTIVE
  To investigate the effect of nHACP/CF on coherence and proliferation of osteoblast.
  
  
  METHODS
  The rat osteoblasts were isolated and cultured, then co-cultured with nHACP/CF in vitro. The cells were observed under cytochemical staining for ALP; the cell-material complex was observed under electronic scanning microscope, MTT was used for detection of cell proliferation,and ALP activity was determined at different times.SPSS 11.5 software package was used for one-way ANOVA.
  
  
  RESULTS
  Cytochemical staining for ALP was positive. The best density of seeding cells was 6x10(5)/ml; osteoblast could attach and extend on nHACP/CF,the proliferation and growth were not affected by nHACP/CF.
  
  
  CONCLUSIONS
  nHACP/CF is a good vehicle for rat osteoblast to grow on.
authors.affiliation: Department of Oral and Maxillofacial Surgery, School of Stomatology,China Medical University. Shenyang 110002, Liaoning Province, China. clare_ning@yahoo.com.cn
authors.forename: Ning-Ning
authors.initials: NN
authors.lastname: Sun
authors.forename: Xu-Kai
authors.initials: XK
authors.lastname: Wang
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.forename: Li
authors.initials: L
authors.lastname: Lu
authors.forename: Shao-Hui
authors.initials: SH
authors.lastname: Huang
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D002478:Cells, Cultured; D003470:Culture Media; D066298:In Vitro Techniques; D010006:Osteoblasts; D051381:Rats
chemical_list: D003470:Culture Media