Establishment of a polymerase chain reaction method for detection of Escherichia coli in amniotic fluid in patients with chorioamnionitis.
OBJECTIVE
We investigated whether Escherichia coli could be detected by E. coli of reference (ECOR) grouping and virulence factors (VFs) in amniotic fluid using polymerase chain reaction (PCR).


METHODS
From 18 patients with clinical symptoms, such as cyclic uterine contraction, genital bleeding and cervical ripening, who subsequently developed abortion or labor before term, and from 40 normal pregnant women undergoing diagnostic amniocentesis, amniotic fluid was obtained. All samples were negative for standard culture. Six patients with symptoms were classified into the ECOR group, and with VFs, E. coli was detected in 6 patients. Thus, 4 patients were positive for both tests.


CONCLUSIONS
We could establish a detection method for E. coli in amniotic fluid using both ECOR grouping and VFs with PCR.
Daoud
Ghada A
GA
Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Nagoya City University Medical School, Nagoya, Japan.
Suzuki
Yoshikatsu
Y
Yamamoto
Tamao
T
Suzuki
Takashi
T
Suzumori
Nobuhiro
N
Tanemura
Mitsuyo
M
D000649:Amniocentesis; D000653:Amniotic Fluid / Q000737:chemistry / Q000382:microbiology*; D016022:Case-Control Studies; D002821:Chorioamnionitis / Q000378:metabolism / Q000382:microbiology*; D016207:Cytokines / Q000032:analysis; D004269:DNA, Bacterial / Q000302:isolation & purification*; D004926:Escherichia coli / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000472:pathogenicity; D005260:Female; D005865:Gestational Age; D005947:Glucose / Q000032:analysis; D006801:Humans; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D037521:Virulence Factors / Q000235:genetics / Q000302:isolation & purification*
D016207:Cytokines; D004269:DNA, Bacterial; D037521:Virulence Factors; D005947:Glucose