Techniques developed in China for foot-and-mouth disease diagnosis.
Over the past several decades, a series of new methods have been developed in China for the diagnosis of foot-and-mouth disease (FMD). These methods are theoretically consistent with Office International des Epizooties standards for FMD diagnosis and include: antigen-capture ELISA and colloid-gold test strip for viral antigen typing, liquid-phase blocking ELISA (LPBE) for detection of antibodies against O and Asia 1 FMDV, and an indirect ELISA for detection of antibodies against the non-structural protein (NSP) 3ABC (3ABC-I-ELISA). Several molecular diagnostic methods have also been developed for detection of fragments of the FMDV genome within viral samples, such as multiplex RT-PCR, typing RT-PCR and real-time RT-PCR. In China, FMD vaccines are compulsorily used in all livestock. In such a situation, monitoring of the immune status of animal herds and disease surveillance become very important. Liquid-phase blocking ELISA is routinely used in China for monitoring the immune status, and 3ABC-I-ELISA is feasible for large-scale serological surveys based on differentiation of FMDV-infected animals from vaccinated animals. Combined use of 3ABC-ELISA and mRT-PCR is described for survey of FMD virus-prevalent herds and virus carriers.
Lu
Z
Z
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, Xujiaping No 1, Yanchangpu, Lanzhou, Gansu 730046, China. liukey@public.lz.gs.cn
Cao
Y
Y
Bao
H
H
Qi
S
S
Guo
J
J
Shang
Y
Y
Jiang
T
T
Zhang
Q
Q
Ma
J
J
Liu
Z
Z
Liu
X
X
Yin
H
H
Xie
Q
Q
D000818:Animals; D002353:Carrier State / Q000662:veterinary; D002681:China; D019411:Clinical Laboratory Techniques / Q000592:standards / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D018571:Sentinel Surveillance / Q000662:veterinary; D014765:Viral Vaccines / Q000008:administration & dosage*
D014765:Viral Vaccines