title: [Change of oil-degrading activity in microorganisms stored under laboratory conditions].
abstract: Change of the oil-degrading activity was studied in psychrophilic microbial strains Rhodococcus spp. DS-07, DS-21 and Pseudomonas spp. DS-09, DS-22 maintained on various media: rich and synthetic with a selective agent. After 2.5 years of storage on rich medium, the oil-degrading activity decreased by 50-60%, whereas this decrease was insignificant in the medium with oil. Passages to selective medium with oil after the storage partly restored the activity. It was found that storage of oil-degrading microorganisms caused loss of biodegradation plasmids. Their recovery and long-term preservation demand the presence of the selective agent in the medium.
authors.forename: N I
authors.initials: NI
authors.lastname: Belousova
authors.forename: L M
authors.initials: LM
authors.lastname: Baryshnikova
authors.forename: A N
authors.initials: AN
authors.lastname: Shkidchenko
mesh_terms: D001673:Biodegradation, Environmental; D010578:Petroleum / Q000378:metabolism*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011309:Preservation, Biological / Q000379:methods; D011549:Pseudomonas / Q000235:genetics / Q000254:growth & development*; D012240:Rhodococcus / Q000235:genetics / Q000254:growth & development*; D013997:Time Factors
chemical_list: D010578:Petroleum