title: [Effects of Scutellaria Barbata drug-containing serum on apoptosis and mitochondrial transmembrane potential of hepatoma H22 cells].
abstract: OBJECTIVE
  To investigate the effects of serum containing Scutellaria Barbata extract (ESB) on apoptosis rate and mitochondrial transmembrane potential (MTP) of liver cancer cell line H22 from mice in vitro.
  
  
  METHODS
  H22 cells were cultured in vitro and divided into 5 groups: blank control group, low-dose ESB group, medium-dose ESB group, high-dose ESB group and fluorouracil (5-Fu) group. Methyl thiazolyl tetrazolium assay was utilized to determine the proliferation rates of H22 cells. Cellular morphology was observed under a transmission electron microscope (EM). The rhodamine 123 was used as a fluorescence probe to label the H22 cells, and the fluorescence intensities were observed with a laser scanning confocal microscope. The fluorescence intensity of H22 cells indicated the MTP of H22 cells.
  
  
  RESULTS
  The inhibition of serum containing ESB on the proliferation of H22 cells in vitro was observed in a time-dependent manner. The typical morphological changes of apoptosis were observed after incubation with ESB-containing serum in high dose for 48h. The apoptosis rates of blank control group, 5-Fu group, low-dose ESB group, medium-dose ESB group and high-dose ESB group were (0.51+/-0.32)%, (11.26+/-2.97)%, (1.07+/-0.46)%, (3.15+/-1.12)%, (7.83+/-2.25)% respectively. ESB could reduce the MTP of H22 cells from mice as compared with the untreated group. The MTPs of the blank control group, 5-Fu group, and low-, medium- and high-dose ESB groups were (245.45+/-67.37), (127.42+/-41.35), (213.68+/-65.52), (186.34+/-56.37) and (142.65+/-39.44) respectively, which were negatively correlated with the apoptosis rates.
  
  
  CONCLUSIONS
  ESB-containing serum effectively induces apoptosis, which may be related to the decrease of MTP in H22 cells.
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi Province 710004, China. dzj0911@126.com
authors.forename: Zhi-jun
authors.initials: ZJ
authors.lastname: Dai
authors.forename: Xi-jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Qian
authors.initials: Q
authors.lastname: Xue
authors.forename: Zong-zheng
authors.initials: ZZ
authors.lastname: Ji
authors.forename: Xiao-xu
authors.initials: XX
authors.lastname: Liu
authors.forename: Hua-feng
authors.initials: HF
authors.lastname: Kang
authors.forename: Hai-tao
authors.initials: HT
authors.lastname: Guan
authors.forename: Xiao-bin
authors.initials: XB
authors.lastname: Ma
authors.forename: Hong-tao
authors.initials: HT
authors.lastname: Ren
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000473:pathology; D045744:Cell Line, Tumor; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects*; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D031364:Scutellaria baicalensis; D044967:Serum
chemical_list: D010936:Plant Extracts; C499814:Scutellaria baicalensis extract