title: Guidelines for the detection of Trichinella larvae at the slaughterhouse in a quality assurance system.
abstract: The European Community Regulation (EC) No. 2075/2005 lays down specific rules on official controls for the detection of Trichinella in fresh meat for human consumption, recommending the pooled-sample digestion method as the reference method. The aim of this document is to provide specific guidance to implement an appropriate Trichinella digestion method by a laboratory accredited according to the ISO/IEC 17025:2005 international standard, and performing microbiological testing following the EA-04/10:2002 international guideline. Technical requirements for the correct implementation of the method, such as the personnel competence, specific equipments and reagents, validation of the method, reference materials, sampling, quality assurance of results and quality control of performance are provided, pointing out the critical control points for the correct implementation of the digestion method.
authors.affiliation: Dipartimento di Malattie Infettive Parassitarie e Immunomediate, Laboratorio Comunitario di Riferimento per i Parassiti, Istituto Superiore di Sanità, Rome, Italy.
authors.forename: Patrizia
authors.initials: P
authors.lastname: Rossi
authors.forename: Edoardo
authors.initials: E
authors.lastname: Pozio
mesh_terms: D000003:Abattoirs / Q000592:standards*; D000818:Animals; D005502:Food / Q000592:standards; D005506:Food Contamination / Q000517:prevention & control; D007202:Indicators and Reagents; D007814:Larva / Q000737:chemistry; D008460:Meat / Q000469:parasitology*; D011786:Quality Control; D015203:Reproducibility of Results; D014234:Trichinella / Q000737:chemistry*
chemical_list: D007202:Indicators and Reagents