title: Mutanase induction in Trichoderma harzianum by cell wall of Laetiporus sulphureus and its application for mutan removal from oral biofilms.
abstract: The cell wall material from fruiting bodies of Laetiporus sulphureus has been suggested as a new alternative to mutan for the mutanase induction in Trichoderma harzianum. Structural analyses revealed that the alkali-soluble wall fraction from this polypore fungus contained 56.3% of (1-->3)-linked alpha-glucans. When the strain T. harzianum F-340 was grown on a cell wall preparation from L. sulphureus, the maximal enzyme productivity obtained after 3 days of cultivation was 0.71 U/ml. This yield was about 1.8-fold higher than that achieved on mutan, known so far as the best, but expensive and inaccessible, inducer of mutanase production. Cell-wall-induced mutanase showed a high hydrolytic potential in reaction with a dextranase-pretreated mutan, where maximal degrees of saccharification and solubilization of this biopolymer (80% and 100%, respectively) were reached in 3 h at 45 degrees C. The mutanase preparation was also effective in degradation of streptococcal mutan and its removal from oral biofilms, especially in a mixture with dextranase.
authors.affiliation: Department of Industrial Microbiology, Maria Curie-Skłodowska University, Akademicka 19, Lublin, Poland. adrianw2@tlen.pl
authors.forename: Adrian
authors.initials: A
authors.lastname: Wiater
authors.forename: Janusz
authors.initials: J
authors.lastname: Szczodrak
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Pleszczyńska
mesh_terms: D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002473:Cell Wall / Q000737:chemistry / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D005936:Glucans / Q000737:chemistry / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology*; D009059:Mouth Diseases / Q000188:drug therapy / Q000382:microbiology; D020072:Polyporales / Q000737:chemistry / Q000378:metabolism*; D013290:Streptococcal Infections / Q000188:drug therapy / Q000382:microbiology; D013295:Streptococcus mutans / Q000187:drug effects / Q000378:metabolism; D014242:Trichoderma / Q000201:enzymology*
chemical_list: D005656:Fungal Proteins; D005936:Glucans; D006026:Glycoside Hydrolases; C021780:exo-1,3-alpha-glucanase