Overexpression of a fatty acid amide hydrolase compromises innate immunity in Arabidopsis.
N-acylethanolamines are a group of lipid mediators that accumulate under a variety of neurological and pathological conditions in mammals. N-acylethanolamine signaling is terminated by the action of diverse hydrolases, among which fatty acid amide hydrolase (FAAH) has been well characterized. Here, we show that transgenic Arabidopsis lines overexpressing an AtFAAH are more susceptible to the bacterial pathogens Pseudomonas syringae pv. tomato and P. syringae pv. maculicola. AtFAAH overexpressors also were highly susceptible to non-host pathogens P. syringae pv. syringae and P. syringae pv. tabaci. AtFAAH overexpressors had lower amounts of jasmonic acid, abscisic acid and both free and conjugated salicylic acid (SA), compared with the wild-type. Gene expression studies revealed that transcripts of a number of plant defense genes, as well as genes involved in SA biosynthesis and signaling, were lower in AtFAAH overexpressors than wild-type plants. Our data suggest that FAAH overexpression alters phytohormone accumulation and signaling which in turn compromises innate immunity to bacterial pathogens.
Kang
Li
L
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
Wang
Yuh-Shuh
YS
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
Uppalapati
Srinivasa Rao
SR
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
Wang
Keri
K
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
Tang
Yuhong
Y
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
Vadapalli
Vatsala
V
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
Venables
Barney J
BJ
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
Chapman
Kent D
KD
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
Blancaflor
Elison B
EB
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
Mysore
Kirankumar S
KS
Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
D000040:Abscisic Acid / Q000378:metabolism; D000581:Amidohydrolases / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007113:Immunity, Innate; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D044224:Pseudomonas syringae / Q000472:pathogenicity; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000378:metabolism; D000099252:Fatty Acid Amide Hydrolases
D000040:Abscisic Acid; D000581:Amidohydrolases; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004983:Ethanolamines; D054883:Oxylipins; D010937:Plant Growth Regulators; D018749:RNA, Plant; D020156:Salicylic Acid; D000099252:Fatty Acid Amide Hydrolases; C022203:N-acylethanolamines; C011006:jasmonic acid