Which DNA damage is likely to be relevant in hormetic responses?
Working under the assumption that hormesis is triggered by specific types of DNA damage, this report focuses on the types of damage which form the signature of ionizing radiation. The key attribute of the signature is the clustering of damage, arising from clusters of energy deposition such that more than one site within a 10 base pair segment of DNA has been chemically altered. A brief overview is given on what is currently believed to be the primary components of clustered damage produced by the direct effect. The overview draws primarily on studies that utilize electron paramagnetic resonance to measure free radical intermediates and gel electrophoresis to measure clustered damage in plasmid DNA. Based on this information, the threshold for a radiation induced biological response is calculated.
Bernhard
William A
WA
Department of Biochemistry and Biophysics, University of Rochester, Rochester, NY 14642, USA. William_bernhard@urmc.rochester.edu
Purkayastha
Shubhadeep
S
Milligan
Jamie R
JR