[Inhibition of growth and proliferation of Hep-2 cells by targeting c-myc gene using small interference RNA technology].
OBJECTIVE
To investigate the effect of small interfering RNA (siRNA) targeting c-myc gene in Hep-2 cells.


METHODS
siRNA targeting c-myc mRNA was designed and synthesized. In vitro cultured Hep-2 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by MTT, morphology, real time PCR assay.


RESULTS
1) The MTT result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication; 2) The real time PCR result showed c-myc at mRNA level inhibition ratio at 94% in group S3; 3) The morphology result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication, the cell heteromorphism was diminished.


CONCLUSIONS
siRNA targeting c-myc can remarkably suppress the Hep-2 cell growth and multiplication.
Tang
Zhi
Z
Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Shunde, Foshan, 528300, China. damaodoctor@126.com
Wang
Jiqun
J
Wu
Xinyi
X
Zhang
Tao
T
Lin
Xueli
X
Shan
Yanchun
Y
D017209:Apoptosis; D002455:Cell Division; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018390:Gene Targeting; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
D034741:RNA, Small Interfering