Immunohistochemical identification of messenger RNA-related proteins in basophilic inclusions of adult-onset atypical motor neuron disease.
This report concerns an immunohistochemical investigation on RNA-related proteins in the basophilic inclusions (BIs) from patients with adult-onset atypical motor neuron disease. Formalin-fixed, paraffin-embedded sections of the motor cortex and the lumbar spinal cord were examined. The BIs appeared blue in color with H&E and Nissl stain, and pink with methylgreen-pyronin stain. Ribonuclease pretreatment abolished the methylgreen-pyronin staining, suggesting that the BIs contained RNA. Immunohistochemically, the BIs were distinctly labeled with the antibodies against poly(A)-binding protein 1, T cell intracellular antigen 1, and ribosomal protein S6. These proteins are essential constituents of stress granules. In contrast, the BIs were not immunoreactive for ribosomal protein L28 and decapping enzyme 1, which are core components of transport ribonucleoprotein particles and processing bodies, respectively. Moreover, the BIs were not immunopositive for TDP-43. Our results imply that translation attenuation could be involved in the processes of BI formation in this disorder.
Fujita
Kengo
K
Department of Neurology, Kansai Medical University, 10-15, Fumizono-cho, Moriguchi, Osaka 570-8507, Japan.
Ito
Hidefumi
H
Nakano
Satoshi
S
Kinoshita
Yoshimi
Y
Wate
Reika
R
Kusaka
Hirofumi
H
D004722:Endoribonucleases / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry; D002479:Inclusion Bodies / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009044:Motor Cortex / Q000378:metabolism / Q000473:pathology; D016472:Motor Neuron Disease / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D039122:Poly(A)-Binding Protein I / Q000378:metabolism*; D039102:Poly(A)-Binding Proteins / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D038601:Ribosomal Protein S6 / Q000378:metabolism*; D012269:Ribosomal Proteins / Q000378:metabolism; D013194:Staining and Labeling; D000074019:T-Cell Intracellular Antigen-1; D015534:Trans-Activators / Q000378:metabolism
D039122:Poly(A)-Binding Protein I; D039102:Poly(A)-Binding Proteins; D012333:RNA, Messenger; C489320:RPL28 protein, human; D038601:Ribosomal Protein S6; D012269:Ribosomal Proteins; D000074019:T-Cell Intracellular Antigen-1; C071558:TIA1 protein, human; D015534:Trans-Activators; D004722:Endoribonucleases; C466279:DCP1A protein, human