title: Effects of combined pulse electromagnetic field stimulation plus glutamine on the healing of colonic anastomosis in rats.
abstract: OBJECTIVE
  An experimental study was designed to investigate the effect of combined pulse electromagnetic field (PEMF) stimulation plus glutamine administration on colonic anastomosis.
  
  
  METHODS
  Anastomosis of the left colon was performed in 28 rats, which were divided into four groups; Group 1: normal resection anastomosis plus oral 50 mg/kg/day glutamine; Group 2: normal resection anastomosis plus PEMF stimulation plus oral 50 mg/kg/day glutamine; Group 3: normal resection anastomosis plus PEMF stimulation; Group 4: normal resection anastomosis. On the seventh postoperative day, the animals were killed and the bursting pressure and tissue hydroxyproline concentration of the anastomosis were analyzed and compared.
  
  
  RESULTS
  The mean anastomotic bursting pressure in Group 2 was significantly higher than in Groups 1 and 4. On the other hand, the mean anastomotic bursting pressure in Group 1 was significantly higher than in Group 4. The collagen deposition and the fibroblast infiltration were significantly increased on the seventh day in Group 3 compared the other groups. On the other hand, Groups 1 and 2 had higher scores for collagen deposition and fibroblast infiltration than Group 4.
  
  
  CONCLUSIONS
  In conclusion, burst pressures, hydroxyproline, and histologic features (fibroblast infiltration and collagen deposition) were improved in the PEMF group, and both PEMF and glutamine-enriched nutrition provide a significant gain in the strength of colonic anastomoses in rats.
authors.affiliation: Department of General Surgery, Medical School, Dicle University, Diyarbakir, Turkey.
authors.forename: Sadullah
authors.initials: S
authors.lastname: Girgin
authors.forename: Ercan
authors.initials: E
authors.lastname: Gedik
authors.forename: Hayrettin
authors.initials: H
authors.lastname: Ozturk
authors.forename: Veysi
authors.initials: V
authors.lastname: Akpolat
authors.forename: Veysi
authors.initials: V
authors.lastname: Akbulut
authors.forename: Ebru
authors.initials: E
authors.lastname: Kale
authors.forename: Huseyin
authors.initials: H
authors.lastname: Buyukbayram
authors.forename: Salih
authors.initials: S
authors.lastname: Celik
mesh_terms: D000714:Anastomosis, Surgical; D000818:Animals; D001696:Biomechanical Phenomena; D003106:Colon / Q000473:pathology / Q000502:physiology / Q000601:surgery*; D005973:Glutamine / Q000627:therapeutic use*; D055909:Magnetic Field Therapy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing
chemical_list: D005973:Glutamine