[The cytological indicators of the overall suppression of protein synthesis as revealed by staining with a new monoclonal antibody].
In this work we describe how the nucleolus reacts to inhibition of protein synthesis as revealed by labeling with a new monoclonal antibody A3. In normal cells A3 antigen is observed as numerous foci within the nucleolus. During mitosis A3 antigen is located in a few foci on chromosomes. Regions of A3 localization are susceptible to pepsin treatment but are not susceptible to RNAse A treatment. This fact indicates that A3 antigen is of protein nature. On the ultra structural level, A3 antigen is localized primarily at the periphery of fibrillar centers. Taken together these properties of A3 antigen suggest that it's a component of the RNA polymerase I transcription machinery. A3 antigen has an intriguing property, namely, an ability to migrate from the nucleolus to the nucleoplasm upon inhibition of protein synthesis with anisomycin, puromycin or cycloheximide. The obtained results show that the localization of A3 antigen revealed by the new monoclonal antibody may serve as a cytological indicator of the overall level of protein synthesis in vitro.
Grigor'ev
A A
AA
Bulycheva
T I
TI
Sheval'
E V
EV
Kalinina
I A
IA
Zatsepina
O V
OV
D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000918:Antibody Specificity; D002466:Cell Nucleolus / Q000737:chemistry* / Q000378:metabolism; D005786:Gene Expression Regulation; D006367:HeLa Cells; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D008807:Mice, Inbred BALB C; D009687:Nuclear Proteins / Q000276:immunology / Q000302:isolation & purification* / Q000378:metabolism; D014176:Protein Biosynthesis; D012318:RNA Polymerase I / Q000302:isolation & purification* / Q000378:metabolism; D014158:Transcription, Genetic
D000911:Antibodies, Monoclonal; D009687:Nuclear Proteins; D012318:RNA Polymerase I