[Inhibitory mechanism of melanin synthesis by glutathione].
Glutathione dose dependently inhibited melanin synthesis in the reaction of tyrosinase and L-DOPA. The inhibition of melanin synthesis was recovered by increasing the concentration of L-DOPA, but not recovered by increasing tyrosinase. Glutathione inhibited the binding between tyrosinase and L-DOPA. Although the synthesized melanin was aggregated within 1 h, the aggregation was inhibited by the addition of glutathione. These results indicate that glutathione inhibits the synthesis and agglutination of melanin by interrupting the function of L-DOPA.
Matsuki
Mitsuo
M
Department of Microbiology, Tohoku Pharmaceutical University, Sendai, Japan.
Watanabe
Toshihiko
T
Ogasawara
Ayako
A
Mikami
Takeshi
T
Matsumoto
Tatsuji
T
D000371:Agglutination; D003864:Depression, Chemical; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000494:pharmacology*; D007980:Levodopa; D008543:Melanins / Q000096:biosynthesis*; D014442:Monophenol Monooxygenase; D011485:Protein Binding / Q000187:drug effects
D008543:Melanins; D007980:Levodopa; D014442:Monophenol Monooxygenase; D005978:Glutathione