Verification of reference genes for relative quantification of gene expression by real-time reverse transcription PCR in the pig.
The aim of this study was to develop a set of reliable reference genes for quantification of mRNA expression in the pig. The mRNA expression stability in pig tissues was studied for 4 genes: EEF1A1, GAPDH, HPRT1 and TOP2B. The level of expression was characterized by Ct values for each gene and each tissue. By using the geNorm algorithm, the stability of the reference genes was determined in the diaphragm, heart, kidney, liver, lungs, longissimus muscle, and spleen. On the basis of this information, suitable reference genes can be selected for mRNA expression studies in relevant pig tissues.
Svobodová
Katerina
K
Department of Animal Morphology, Physiology and Genetics, Mendel University of Agriculture and Forestry, Brno, Czech Republic. kat.svobodova@centrum.cz
Bílek
Karel
K
Knoll
Ales
A
D000818:Animals; D004250:DNA Topoisomerases, Type II / Q000235:genetics* / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+) / Q000235:genetics* / Q000378:metabolism; D007041:Hypoxanthine Phosphoribosyltransferase / Q000235:genetics* / Q000378:metabolism; D020648:Peptide Elongation Factor 1 / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013552:Swine / Q000235:genetics*; D014018:Tissue Distribution
D004268:DNA-Binding Proteins; D020648:Peptide Elongation Factor 1; D012333:RNA, Messenger; D024601:Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+); D007041:Hypoxanthine Phosphoribosyltransferase; D004250:DNA Topoisomerases, Type II