title: Optimizing sample handling for urinary proteomics.
abstract: Interrogation of the urinary proteome for clinically useful biomarkers of disease will require normalization of methods for protein extraction and sample handling. Variations in collection methods and other procedures may introduce significant discrepancies in qualitative and quantitative measurements. Here we demonstrate that the method of protein extraction, length of handling at room temperature, and repetitive freeze-thaw cycles do not seem to alter the urinary proteome at either the protein or peptide level in a manner that degrades information obtainable by mass spectrometry.
authors.affiliation: Department of Urology, Urological Diseases Research Center, Harvard Medical School and Children's Hospital Boston, Boston, Massachusetts 02115, USA. richard.lee@childrens.harvard.edu
authors.forename: Richard S
authors.initials: RS
authors.lastname: Lee
authors.forename: Flavio
authors.initials: F
authors.lastname: Monigatti
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Briscoe
authors.forename: Zachary
authors.initials: Z
authors.lastname: Waldon
authors.forename: Michael R
authors.initials: MR
authors.lastname: Freeman
authors.forename: Hanno
authors.initials: H
authors.lastname: Steen
mesh_terms: D000328:Adult; D011232:Chemical Precipitation; D015180:Electrophoresis, Gel, Two-Dimensional; D005615:Freezing; D006801:Humans; D008297:Male; D011506:Proteins / Q000302:isolation & purification*; D011507:Proteinuria / Q000652:urine*; D040901:Proteomics; D014462:Ultrafiltration
chemical_list: D011506:Proteins