title: [Effects of RNA elements within 3'untranslated region on dengue virus translation].
abstract: OBJECTIVE
  To investigate the effect of the well-defined RNA elements (VR, RCS2, CS2, CS1 and SL) within the 3'untranslated region (UTR) on dengue virus (DEN) translation.
  
  
  METHODS
  We constructed a virus induced reporter gene (VIRG) by inserting the firefly luciferase (LUC) gene between 5'- and 3'-UTRs of DEN2-43 genome. Subsequently, a series of modified VIRGs consisting of different RNA elements in the 3'UTR were constructed. A 3'UTR-deficient VIRG was also constructed. The translational efficiency of all VIRGs was then analyzed by LUC detection, real time RT-PCR and Western blot assays.
  
  
  RESULTS
  The translation of 3'UTR-deficient VIRG was abolished. The translational efficiency of VIRG with RNA element VR was comparable with that of VIRG with unmodified 3'UTR. The translational efficiency of VIRG with RNA elements RCS2 or CS2 was significantly higher while the translational efficiency of VIRG with RNA elements CS1 or SL was substantially lower than that of VIRG with RNA element VR.
  
  
  CONCLUSIONS
  These results suggested that 3'UTR was indispensable for DEN translation, and some RNA elements within 3'UTR might either up-regulate or down- regulate translation.
authors.affiliation: State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wei
authors.forename: Tao
authors.initials: T
authors.lastname: Jiang
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.forename: Zhongyu
authors.initials: Z
authors.lastname: Liu
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Deng
authors.forename: Ran
authors.initials: R
authors.lastname: Liu
authors.forename: Chengfeng
authors.initials: C
authors.lastname: Qin
authors.forename: E
authors.initials: E
authors.lastname: de Qin
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D002460:Cell Line; D003716:Dengue Virus / Q000235:genetics*; D015967:Gene Expression Regulation, Viral; D017930:Genes, Reporter / Q000235:genetics; D005822:Genetic Vectors / Q000235:genetics; D014176:Protein Biosynthesis / Q000235:genetics*; D020871:RNA Stability; D012367:RNA, Viral / Q000235:genetics*
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral