Morphological and functional characterizations of Schwann cells stimulated with Mycobacterium leprae.
Nerve damage, a characteristic of leprosy, is the cause of patient deformities and a consequence of Schwann cells (SC) infection by Mycobacterium leprae. Although function/dysfunction of SC in human diseases like leprosy is difficult to study, many in vitro models, including SC lines derived from rat and/or human Schwannomas, have been employed. ST88-14 is one of the cell lineages used by many researchers as a model for M. leprae/SC interaction. However, it is necessary to establish the values and limitations of the generated data on the effects of M. leprae in these SC. After evaluating the cell line phenotype in the present study, it is close to non-myelinating SC, making this lineage an ideal model for M. leprae/SC interaction. It was also observed that both M. leprae and PGL-1, a mycobacterial cell-wall component, induced low levels of apoptosis in ST88-14 by a mechanism independent of Bcl-2 family members.
Silva
Tatiana Pereira da
TP
Laboratório de Patologia, Instituto Oswaldo Cruz-Fiocruz, Rio de Janeiro, Brasil.
Silva
Ana Caroline Costa da
AC
Baruque
Maria da Graça Araújo
Mda G
Oliveira
Rosane Barbosa de
RB
Machado
Marcelo Pelajo
MP
Sarno
Euzenir Nunes
EN
D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism*; D017209:Apoptosis / Q000502:physiology*; D045744:Cell Line, Tumor; D005434:Flow Cytometry; D006017:Glycolipids / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D051379:Mice; D009166:Mycobacterium leprae / Q000502:physiology*; D010641:Phenotype; D016133:Polymerase Chain Reaction; D011817:Rabbits; D012583:Schwann Cells / Q000378:metabolism / Q000382:microbiology* / Q000502:physiology
D000942:Antigens, Bacterial; D006017:Glycolipids; C045169:phenolic glycolipid I, Mycobacterium leprae