In vitro cytotoxic activity of Salsola oppositifolia Desf. (Amaranthaceae) in a panel of tumour cell lines.
The aim of the present study was to evaluate for the first time the in vitro cytotoxic activity of fractions and isolated flavonols from Salsola oppositifolia Desf. (Amaranthaceae). The n-hexane fraction demonstrated an effective cytotoxic activity on the large lung carcinoma and amelanotic melanoma cell lines with IC50 values of 19.1 microg/ml and 24.4 microg/ml, respectively. Also the dichloromethane fraction exhibited cytotoxic activity against COR-L23 (IC50 30.4 microg/ml) and C32 (IC50 33.2 microg/ml) cells, while the EtOAc fraction demonstrated a selective cytotoxic activity against MCF-7 cells (IC50 67.9 microg/ml). The major active constituents of this fraction were isorhamnetin-3-O-glucoside (1) and isorhamnetin-3-O-rutinoside (2), which showed an interesting activity against the cell line MCF-7 with IC50 values of 18.2 and 25.2 microg/ml, respectively. Compound 2 exhibited a strong activity against the hormone-dependent prostate carcinoma LNCaP cell line with an IC50 of 20.5 microg/ml. Constituents of S. oppositifolia were identified by GC-MS and NMR analyses.
Tundis
Rosa
R
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Nutrition and Health Sciences, University of Calabria, I-87030 Rende (CS), Italy. tundis@unical.it
Loizzo
Monica R
MR
Bonesi
Marco
M
Menichini
Federica
F
Statti
Giancarlo A
GA
Menichini
Francesco
F
D027703:Amaranthaceae / Q000737:chemistry; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007680:Kidney Neoplasms; D008545:Melanoma; D000432:Methanol; D017239:Paclitaxel / Q000302:isolation & purification / Q000494:pharmacology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D031248:Salsola / Q000737:chemistry*; D014747:Vinblastine / Q000302:isolation & purification / Q000494:pharmacology
D000970:Antineoplastic Agents; D010636:Phenols; D010936:Plant Extracts; D014747:Vinblastine; D017239:Paclitaxel; D000432:Methanol