Evaluating the function of matriptase and N-acetylglucosaminyltransferase V in prostate cancer metastasis.
Previous studies have identified a subclone cell line (PC-J) which was isolated from a metastatic human prostate cell line, PC-3. In vitro matrigel invasion assays and xenograft animal studies suggested that matriptase was a putative metastatic gene in human prostate carcinoma cells. Although low metastatic prostate tumor cells, LNCaP, also expressed high levels of matriptase mRNA, gelatin zymography indicated that LNCaP cells had extremely low matriptase activity. Further studies using RT-PCR and lectin blotting assays revealed that the expression of N-acetylglucosaminyltransferase V (MGAT5), a glycoprotein that stabilizes matriptase, was low in LNCaP cells compared to PC-3 and PC-J cells. The transient overexpression of MGAT5 significantly enhanced the activity of matriptase and the invasion ability in the LNCaP cells. Knock-down of MGAT5 in PC-3 cells attenuated the metastatic ability of the cells, as determined by the in vitro invasion assay and the xenograft animal studies. Matriptase and MGAT5 may play important role in the metastasis of prostate cancer.
Tsui
Ke-Hung
KH
Department of Urology, Chang Gung Memorial Hospital, Kwei-Shan, Tao-Yuan, Taiwan, ROC.
Chang
Phei-Lang
PL
Feng
Tsui-Hsia
TH
Chung
Li-Chuan
LC
Sung
Hsin-Ching
HC
Juang
Horng-Heng
HH
D000818:Animals; D045744:Cell Line, Tumor; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D017351:N-Acetylglucosaminyltransferases / Q000096:biosynthesis* / Q000235:genetics; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000201:enzymology* / Q000235:genetics / Q000473:pathology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012697:Serine Endopeptidases / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D014162:Transfection
D017351:N-Acetylglucosaminyltransferases; C052254:alpha-1,6-mannosylglycoprotein beta 1,6-N-acetylglucosaminyltransferase; D012697:Serine Endopeptidases; C485596:ST14 protein, human