title: A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis.
abstract: The human MOCS3 gene encodes a protein involved in activation and sulfuration of the C terminus of MOCS2A, the smaller subunit of the molybdopterin (MPT) synthase. MPT synthase catalyzes the formation of the dithiolene group of MPT that is required for the coordination of the molybdenum atom in the last step of molybdenum cofactor (Moco) biosynthesis. The two-domain protein MOCS3 catalyzes both the adenylation and the subsequent generation of a thiocarboxylate group at the C terminus of MOCS2A by its C-terminal rhodanese-like domain (RLD). The low activity of MOCS3-RLD with thiosulfate as sulfur donor and detailed mutagenesis studies showed that thiosulfate is most likely not the physiological sulfur source for Moco biosynthesis in eukaryotes. It was suggested that an L-cysteine desulfurase might be involved in the sulfuration of MOCS3 in vivo. In this report, we investigated the involvement of the human L-cysteine desulfurase Nfs1 in sulfur transfer to MOCS3-RLD. A variant of Nfs1 was purified in conjunction with Isd11 in a heterologous expression system in Escherichia coli, and the kinetic parameters of the purified protein were determined. By studying direct protein-protein interactions, we were able to show that Nfs1 interacted specifically with MOCS3-RLD and that sulfur is transferred from L-cysteine to MOCS3-RLD via an Nfs1-bound persulfide intermediate. Because MOCS3 was shown to be located in the cytosol, our results suggest that cytosolic Nfs1 has an important role in sulfur transfer for the biosynthesis of Moco.
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam, Germany and the.
authors.forename: Zvonimir
authors.initials: Z
authors.lastname: Marelja
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam, Germany and the.
authors.forename: Walter
authors.initials: W
authors.lastname: Stöcklein
authors.affiliation: Helmholtz Center for Infection Research, 38124 Braunschweig, Germany.
authors.forename: Manfred
authors.initials: M
authors.lastname: Nimtz
authors.affiliation: Institute of Biochemistry and Biology, University of Potsdam, D-14476 Potsdam, Germany and the. Electronic address: sleim@uni-potsdam.de.
authors.forename: Silke
authors.initials: S
authors.lastname: Leimkühler
mesh_terms: D013437:Carbon-Sulfur Lyases / Q000737:chemistry / Q000378:metabolism / Q000502:physiology*; D002384:Catalysis; D003067:Coenzymes / Q000096:biosynthesis*; D003593:Cytoplasm / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D007700:Kinetics; D008667:Metalloproteins / Q000096:biosynthesis*; D008954:Models, Biological; D000090006:Molybdenum Cofactors; D009713:Nucleotidyltransferases / Q000378:metabolism*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011621:Pteridines; D013455:Sulfur / Q000378:metabolism; D013466:Sulfurtransferases / Q000378:metabolism*; D020349:Surface Plasmon Resonance; D013885:Thiosulfates / Q000737:chemistry
chemical_list: D013437:Carbon-Sulfur Lyases; D003067:Coenzymes; D008667:Metalloproteins; D000090006:Molybdenum Cofactors; D009713:Nucleotidyltransferases; D011621:Pteridines; D013455:Sulfur; D013466:Sulfurtransferases; D013885:Thiosulfates; C022261:molybdopterin; C502790:MOCS3 protein, human; C513492:NFS1 protein, human; C080546:cysteine desulfurase