Three seedborne cryptic viruses containing double-stranded RNA isolated from white clover.
White clover cryptic viruses (WCCV) 1, 2, and 3 were purified from apparently healthy plants with yields of about 200 mug of virus/kg of tissue. After isopycnic centrifugation in CsCl, WCCV 1 and 2 formed homogeneous bands at densities of 1.392 and 1.375 g/ml, respectively, whereas WCCV 3 was not recovered. Particles of WCCV 1 and 3 were 34 nm in diameter, without obvious morphological subunits, whereas WCCV 2 particles were 38 nm in diameter, with prominent morphological subunits. Antisera with titers up to 1:2048 were obtained against WCCV 1 and 2. Each of the three viruses contained two segments of linear dsRNA (Mr from 1.03 to 1.49 X 10(-6). Virus-like particles, probably those of WCCV 1 and 2 were detected in low numbers in the cytoplasm of about 1/10 of the parenchyma cells of carrier plants examined by thin sectioning. The particles of WCCV 1 and 2 were not transmitted by grafting or mechanical inoculation, but were seed transmitted. Evidence is given that WCCV 1 and 2 multiply in their carrier plants, and are not viruses of associated parasitic or commensal organisms.
Boccardo
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Istituto di Fitovirologia applicator del C.N.R., via O. Vigliani 104, 10135 Torino, Italy.
Milne
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Luisoni
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Lisa
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Accotto
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