Epidemiology of invasive methicillin-resistant Staphylococcus aureus clones collected in France in 2006 and 2007.
We conducted a prospective multicenter study of methicillin-resistant Staphylococcus aureus (MRSA) isolates, including the first five consecutive clinical isolates, collected between September 2006 and February 2007 in 23 hospitals located throughout France (Fig. 1). The 111 isolates were tested for their antibiotic susceptibility patterns and were extensively characterized by screening for drug resistance and agr alleles, multilocus sequence typing (ST), staphylococcal cassette chromosome mec (SCCmec) typing, spa typing, and PCR profiling of 21 toxin genes. Clones were designated by their ST followed by their SCCmec type (I to VI). The Lyon clone ST8-IV or ST8-IV(variant) (n = 77; 69.4%) was widely distributed. Four minor clones were also detected, namely, the "classical" Pediatric clone ST5-IV (n = 9; 8.1%), the "new" Pediatric clone ST5-VI (n = 8; 7.2%), the clone Geraldine ST5-I(truncated) (n = 7; 6.3%), and the European clone ST80-IV (n = 4; 3.6%). The six other isolates were related to five rare clones. Relative to that of other European countries, the situation in France is marked by the predominance of a specific major clone and the worrying emergence of minor clones with enhanced virulence and new antibiotic susceptibility profiles.
Dauwalder
Olivier
O
Centre National de Référence des Staphylocoques, Université Lyon, Lyon F-69372, France.
Lina
Gérard
G
Durand
Géraldine
G
Bes
Michèle
M
Meugnier
Hélène
H
Jarlier
Vincent
V
Coignard
Bruno
B
Vandenesch
François
F
Etienne
Jerome
J
Laurent
Frédéric
F
D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D015373:Bacterial Typing Techniques; D016000:Cluster Analysis; D003428:Cross Infection / Q000453:epidemiology* / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005602:France / Q000453:epidemiology; D005838:Genotype; D006761:Hospitals; D006801:Humans; D016106:Methicillin Resistance; D008826:Microbial Sensitivity Tests; D017720:Molecular Epidemiology; D011110:Polymorphism, Genetic; D011446:Prospective Studies; D017422:Sequence Analysis, DNA / Q000379:methods; D013203:Staphylococcal Infections / Q000453:epidemiology* / Q000382:microbiology*; D013205:Staphylococcal Protein A / Q000235:genetics; D013211:Staphylococcus aureus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D015534:Trans-Activators / Q000235:genetics
C095698:Agr protein, Staphylococcus aureus; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D004269:DNA, Bacterial; D013205:Staphylococcal Protein A; D015534:Trans-Activators