Introducing Black-Gold II, a highly soluble gold phosphate complex with several unique advantages for the histochemical localization of myelin.
A novel gold phosphate complex called Black-Gold II with improved myelin staining properties has been developed. It differs from its predecessor, Black-Gold, in that it is highly water soluble at room temperature. This unique physical property confers a number of advantages for the high resolution staining of myelinated fibers. Specifically, it 1) allows for easier solution preparation, eliminating the need for extended heating or sonicating; 2) produces a more uniform and consistent tracer concentration, resulting in more consistent staining and 3) can be used at a 50% higher concentration, resulting in faster and more intense staining without the need for subsequent treatment with gold chloride intensifiers. To characterize the stain, both normal rat brains as well as those exposed to the neurotoxins kainic acid or methamphetamine were examined. The study also incorporates the first application of such stains to examine peripheral nerves of control and acrylamide-exposed rats.
Schmued
Larry
L
Division of Neurotoxicology, National Center for Toxicological Research/FDA, Jefferson AR 72079, USA. larry.schmued@fda.hhs.gov
Bowyer
John
J
Cozart
Matthew
M
Heard
David
D
Binienda
Zbigniew
Z
Paule
Merle
M
D000661:Amphetamine; D000818:Animals; D005452:Fluoresceins; D007608:Kainic Acid; D009186:Myelin Sheath / Q000378:metabolism / Q000473:pathology*; D009410:Nerve Degeneration / Q000150:complications / Q000378:metabolism / Q000473:pathology*; D009930:Organic Chemicals; D010523:Peripheral Nervous System Diseases / Q000139:chemically induced / Q000150:complications / Q000473:pathology*; D010710:Phosphates; D051381:Rats
C121848:Black-Gold; D005452:Fluoresceins; D009930:Organic Chemicals; D010710:Phosphates; C435731:fluoro jade; D000661:Amphetamine; D007608:Kainic Acid