Characterization of soluble complexes of the Shigella flexneri type III secretion system ATPase.
The conserved ATPase of type III secretion systems is critical to the export of substrates through the apparatus. We present a characterization of the native T3SS ATPase, Spa47, from the cytoplasm of Shigella flexneri, demonstrating it to be in two distinct high-molecular-weight complexes with Spa33: MxiN and MxiK.
Johnson
Steven
S
Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
Blocker
Ariel
A
D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D011485:Protein Binding; D012763:Shigella flexneri / Q000201:enzymology*
D026901:Membrane Transport Proteins; D000251:Adenosine Triphosphatases; C478167:Spa47 protein, Shigella flexneri