title: Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood.
abstract: Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35-40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aquabacterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides-clostridium-lactobacillus cluster, which is indigenous to gastrointestinal flora.
authors.affiliation: Nakamura Gakuen University, Faculty of Nutritional Sciences, Fukuoka, Japan.
authors.forename: Kosei
authors.initials: K
authors.lastname: Moriyama
authors.forename: Chie
authors.initials: C
authors.lastname: Ando
authors.forename: Kosuke
authors.initials: K
authors.lastname: Tashiro
authors.forename: Satoru
authors.initials: S
authors.lastname: Kuhara
authors.forename: Seiichi
authors.initials: S
authors.lastname: Okamura
authors.forename: Shuji
authors.initials: S
authors.lastname: Nakano
authors.forename: Yasumitsu
authors.initials: Y
authors.lastname: Takagi
authors.forename: Takeyoshi
authors.initials: T
authors.lastname: Miki
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Nakashima
authors.forename: Hideki
authors.initials: H
authors.lastname: Hirakawa
mesh_terms: D016470:Bacteremia / Q000175:diagnosis*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S