title: Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes.
abstract: Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21(st) century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.
authors.affiliation: Department of Studies in Applied Botany and Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, India.
authors.forename: Sa
authors.initials: S
authors.lastname: Deepak
authors.forename: Kr
authors.initials: K
authors.lastname: Kottapalli
authors.forename: R
authors.initials: R
authors.lastname: Rakwal
authors.forename: G
authors.initials: G
authors.lastname: Oros
authors.forename: Ks
authors.initials: K
authors.lastname: Rangappa
authors.forename: H
authors.initials: H
authors.lastname: Iwahashi
authors.forename: Y
authors.initials: Y
authors.lastname: Masuo
authors.forename: Gk
authors.initials: G
authors.lastname: Agrawal