Genus-specific PCR for molecular identification of novel isolates of the genus Nesterenkonia.
OBJECTIVE
For rapid identification of Nesterenkonia species, one set of genus-specific primers was designed and synthesized for polymerase chain reaction based on the 16S rRNA gene sequences.


CONCLUSIONS
The genus specificity of these primers was validated with reference strains as well as with wild-type isolates. Partial sequencing results of 16S rRNA gene of the wild-type isolates confirmed that they are members of the genus Nesterenkonia.
Zhi
Xiaoyang
X
Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China.
Yang
Lingling
L
Wang
Yun
Y
Tang
Shukun
S
Lou
Kai
K
Li
Wenjun
W
D044822:Biodiversity; D017931:DNA Primers / Q000235:genetics; D008835:Micrococcaceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
D017931:DNA Primers; D012336:RNA, Ribosomal, 16S