title: Effect of Bacillus anthracis virulence factors on human dendritic cell activation.
abstract: Bacillus anthracis possesses three primary virulence factors: capsule, lethal toxin (LT), and edema toxin (ET). Dendritic cells (DCs) are critical to innate and acquired immunity and represent potential targets for these factors. We examined the ability of B. anthracis spores and bacilli to stimulate human monocyte-derived DC (MDDC), primary myeloid DC (mDC), and plasmacytoid DC (pDC) cytokine secretion. Exposure of MDDCs and mDCs to spores or vegetative bacilli of the genetically complete strain UT500 induced significantly increased cytokine secretion. Spores lacking genes required for capsule biosynthesis stimulated significantly higher cytokine secretion than UT500 spores from mDCs, but not MDDCs. In contrast, bacilli lacking capsule stimulated significantly higher cytokine secretion than UT500 bacilli in both MDDCs and mDCs. Spores or bacilli lacking both LT and ET stimulated significantly higher cytokine secretion than UT500 spores or bacilli, respectively, in both mDCs and MDDCs. pDCs exposed to spores or bacilli did not produce significant amounts of cytokines even when virulence factors were absent. In conclusion, B. anthracis employs toxins as well as capsule to inhibit human MDDC and mDC cytokine secretion, whereas human pDCs respond poorly even when capsule or both toxins are absent.
authors.affiliation: Center for Infectious Disease and Immunity, University of New Mexico Health Science Center, Albuquerque, NM, USA.
authors.forename: Andrew C
authors.initials: AC
authors.lastname: Hahn
authors.forename: C Rick
authors.initials: CR
authors.lastname: Lyons
authors.forename: Mary F
authors.initials: MF
authors.lastname: Lipscomb
mesh_terms: D001408:Bacillus anthracis / Q000378:metabolism / Q000472:pathogenicity*; D016207:Cytokines / Q000096:biosynthesis / Q000276:immunology / Q000378:metabolism*; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D006801:Humans; D013171:Spores, Bacterial / Q000378:metabolism / Q000472:pathogenicity*; D037521:Virulence Factors / Q000276:immunology* / Q000378:metabolism
chemical_list: D016207:Cytokines; D037521:Virulence Factors