[Effect of bone morphogenetic protein-2 on the expression of membrane Type 1-matrix metalloproteinase in human A549 lung carcinoma cells].
OBJECTIVE
To explore the effect of bone morphogenetic protein-2 (BMP-2 ) on the expression of membrane Type 1-matrix metalloproteinase (MT1-MMP) and the activation of matrix metalloproteinase-2 (MMP-2) in human A549 lung carcinoma cells.


METHODS
Western blot was used to analyse the expression of MT1-MMP protein levels in A549 cells. Enzyme-linked immunosorbentassay was used to show the actions of BMP-2 on the activation of MMP-2 in A549 cells.


RESULTS
Treatment with BMP-2 in A549 cells caused a dose- and time-dependent increase in the expression of MT1-MMP protein. BMP-2 dose-dependently activated MMP-2 in A549 cells.


CONCLUSIONS
BMP-2 might promote MT1-MMP expression and MMP-2 activation in lung carcinoma cells, which can lead to the increase of extracellular matrix followed by acceleration of tumor cell migration and invasion. It is probably another key mechanism of BMP-2 upregulated lung carcinoma cell migration.
Tang
Si-Yuan
SY
School of Nursing, Central South University, Changsha 410013, China. tangsyuan@yahoo.com.cn
D055396:Bone Morphogenetic Protein 2 / Q000494:pharmacology*; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology; D020781:Matrix Metalloproteinase 1 / Q000096:biosynthesis*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D014407:Tumor Cells, Cultured
D055396:Bone Morphogenetic Protein 2; D020778:Matrix Metalloproteinase 2; D020781:Matrix Metalloproteinase 1