title: Computationally Optimised DNA Assembly of synthetic genes.
abstract: Gene synthesis is hampered by two obstacles: improper assembly of oligonucleotides; oligonucleotide defects incurred during chemical synthesis. To overcome the first problem, we describe the employment of a Computationally Optimised DNA Assembly (CODA) algorithm that uses the degeneracy of the genetic code to design overlapping oligonucleotides with thermodynamic properties for self-assembly into a single, linear, DNA product. To address the second problem, we describe a hierarchical assembly strategy that reduces the incorporation of defective oligonucleotides into full-length gene constructs. The CODA algorithm and these biological methods enable fast, simple and reliable assemblies of sequence-correct full-length genes.
authors.affiliation: CODA Genomics Inc., Laguna Hills, CA 92653, USA. Larsen@cantab.net
authors.forename: Liza S Z
authors.initials: LS
authors.lastname: Larsen
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Wassman
authors.forename: G Wesley
authors.initials: GW
authors.lastname: Hatfield
authors.forename: Richard H
authors.initials: RH
authors.lastname: Lathrop
mesh_terms: D000465:Algorithms; D001483:Base Sequence; D003198:Computer Simulation; D004247:DNA / Q000737:chemistry* / Q000235:genetics*; D005813:Genes, Synthetic / Q000235:genetics*; D005818:Genetic Engineering / Q000379:methods*; D008956:Models, Chemical; D008957:Models, Genetic; D008969:Molecular Sequence Data; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D004247:DNA