[Effect of Zn2+ on level of IL-8 and TNF-alpha mRNA and protein expressed of human II alveolar epithelial cells].
OBJECTIVE
To study the effect of Zn2+ on levels of the cytokines IL-8 and TNF-alpha mRNA expression of human II alveolar epithelial cell.


METHODS
Human II alveolar epithelial A549 cells were treated with Zn2+ at the doses of 0, 50, 100 and 250 micromol/L Zn2+ for 3 and 24 h, and the mRNA and protein level of IL-8 and TNF-alpha were determined by semiquantitive RT-RCR and RIA, respectively.


RESULTS
Incubation of A549 cells with Zn2+ for 3 h and 24h could stimulate the accumulations of IL-8 and TNF-alpha mRNA and protein of A549 cells in a concentration-dependent manner.


CONCLUSIONS
The production of IL-8 and TNF-alpha mRNA and protein from A549 cells stimulated by Zn2+ could be important source of cytokines in respiratory damage induced by Zn2+.
Zhang
Qiao
Q
Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. zhangqiao@zzu.edu.cn
Yang
Haijun
H
Wu
Weidong
W
Shi
Songhe
S
D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D016209:Interleukin-8 / Q000235:genetics / Q000378:metabolism*; D011650:Pulmonary Alveoli / Q000166:cytology* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism*; D015032:Zinc / Q000494:pharmacology*
C508606:CXCL8 protein, human; D016209:Interleukin-8; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha; D015032:Zinc