title: Mapping of markers related to self-incompatibility, disease resistance, and quality traits in Lolium perenne L.
abstract: Several linkage maps, mainly based on anonymous markers, are now available for Lolium perenne. The saturation of these maps with markers derived from expressed sequences would provide information useful for QTL mapping and map alignment. Therefore, we initiated a study to develop and map DNA markers in genes related to self-incompatibility, disease resistance, and quality traits such as digestibility and sugar content in two L. perenne families. In total, 483 and 504 primer pairs were designed and used to screen the ILGI and CLO-DvP mapping populations, respectively, for length polymorphisms. Finally, we were able to map 67 EST markers in at least one mapping population. Several of these markers coincide with previously reported QTL regions for the traits considered or are located in the neighbourhood of the self-incompatibility loci, S and Z. The markers developed expand the set of gene-derived markers available for genetic mapping in ryegrasses.
authors.affiliation: Institute for Agricultural and Fisheries Research, Plant Growth and Development, Caritasstraat 21, 9090 Melle, Belgium.
authors.forename: Inge
authors.initials: I
authors.lastname: Van Daele
authors.forename: Hilde
authors.initials: H
authors.lastname: Muylle
authors.forename: Erik
authors.initials: E
authors.lastname: Van Bockstaele
authors.forename: Isabel
authors.initials: I
authors.lastname: Roldán-Ruiz
mesh_terms: D015415:Biomarkers; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D020224:Expressed Sequence Tags; D008040:Genetic Linkage; D007113:Immunity, Innate / Q000235:genetics*; D008129:Lolium / Q000235:genetics* / Q000276:immunology; D040641:Quantitative Trait Loci
chemical_list: D015415:Biomarkers