title: RIG-I plays a critical role in negatively regulating granulocytic proliferation.
abstract: RIG-I has been implicated in innate immunity by sensing intracellular viral RNAs and inducing type I IFN production. However, we have found a significant RIG-I induction in a biological setting without active viral infection-namely, during RA-induced terminal granulocytic differentiation of acute myeloid leukemias. Here, we present evidence that a significant Rig-I induction also occurs during normal myelopoiesis and that the disruption of the Rig-I gene in mice leads to the development of a progressive myeloproliferative disorder. The initiation of progressive myeloproliferative disorder is mainly due to an intrinsic defect of Rig-I(-/-) myeloid cells, which are characterized by a reduced expression of IFN consensus sequence binding protein, a major regulator of myeloid differentiation. Thus, our study reveals a critical regulatory role of Rig-I in modulating the generation and differentiation of granulocytes.
authors.affiliation: State Key Laboratory for Medical Genomics, Institute of Health Science, Shanghai Institute for Biological Sciences and Graduate School, Chinese Academy of Sciences, People's Republic of China.
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Zhang
authors.forename: Shu-Hong
authors.initials: SH
authors.lastname: Shen
authors.forename: Lin-Jia
authors.initials: LJ
authors.lastname: Jiang
authors.forename: Wu
authors.initials: W
authors.lastname: Zhang
authors.forename: Hong-Xin
authors.initials: HX
authors.lastname: Zhang
authors.forename: Yue-Ping
authors.initials: YP
authors.lastname: Sun
authors.forename: Xian-Yang
authors.initials: XY
authors.lastname: Li
authors.forename: Qiu-Hua
authors.initials: QH
authors.lastname: Huang
authors.forename: Bao-Xue
authors.initials: BX
authors.lastname: Ge
authors.forename: Sai-Juan
authors.initials: SJ
authors.lastname: Chen
authors.forename: Zhu-Gang
authors.initials: ZG
authors.lastname: Wang
authors.forename: Zhu
authors.initials: Z
authors.lastname: Chen
authors.forename: Jiang
authors.initials: J
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases / Q000502:physiology*; D005091:Exons; D005786:Gene Expression Regulation; D006098:Granulocytes / Q000166:cytology* / Q000378:metabolism; D006412:Hematopoietic Stem Cells / Q000166:cytology; D006801:Humans; D007113:Immunity, Innate; D051379:Mice; D018345:Mice, Knockout; D008822:Mice, Transgenic; D022423:Myeloid Cells / Q000166:cytology; D009196:Myeloproliferative Disorders / Q000378:metabolism; D018168:Receptors, Retinoic Acid / Q000502:physiology*
chemical_list: C437968:PLAAT4 protein, human; D018168:Receptors, Retinoic Acid; C503356:Ddx58 protein, mouse; D000071457:DEAD Box Protein 58; D053487:DEAD-box RNA Helicases