title: [mRNA expression level of nucleostemin in esophageal squamous cell carcinoma tissue].
abstract: OBJECTIVE
  To investigate the mRNA expression levels of nucleostemin (NS) in human esophageal squamous cell carcinoma tissue.
  
  
  METHODS
  Real-time PCR was used to quantify the mRNA expression of NS in the samples of esophageal squamous cell carcinoma tissue and their matched normal esophageal mucosa tissue from 62 patients, 36 males and 26 females, aged (61 +/- 10) (38-75). The relationship between NS mRNA expression level and clinical pathological features was analyzed.
  
  
  RESULTS
  The NS mRNA expression level of the 62 cases of esophageal squamous cell carcinoma tissue was(4.5 +/- 2.1), significantly higher than that of the matched normal esophageal mucosa tissue [(2.1 +/- 1.3), t = -5.045, P = 0.000]. The mRNA expression level of NS was associated with tumor grade, depth of infiltration, and lymph node metastasis (all P < 0.05), but not with gender, age, and pathological type (all P > 0.05). Multiple linear regression analysis revealed that clinical and pathological features influenced the NS mRNA expression level (P = 0. 000), and the depth of infiltration and lymph node metastasis were important influencing factors for NS mRNA expression level(both P < 0.05).
  
  
  CONCLUSIONS
  NS may play an important role in the progression and proliferation of esophageal squamous cell carcinoma.
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China.
authors.forename: Gong-yuan
authors.initials: GY
authors.lastname: Zhang
authors.forename: Ping-ping
authors.initials: PP
authors.lastname: Chen
authors.forename: Sheng-lei
authors.initials: SL
authors.lastname: Li
authors.forename: Lei
authors.initials: L
authors.lastname: Yin
authors.forename: Dong-ling
authors.initials: DL
authors.lastname: Gao
authors.forename: Xiao-ping
authors.initials: XP
authors.lastname: Le
authors.forename: Kui-sheng
authors.initials: KS
authors.lastname: Chen
authors.forename: Yun-han
authors.initials: YH
authors.lastname: Zhang
authors.forename: Qin-xian
authors.initials: QX
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D002352:Carrier Proteins / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology*; D005260:Female; D019204:GTP-Binding Proteins; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D002352:Carrier Proteins; C469949:GNL3 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D019204:GTP-Binding Proteins