Mango starch degradation. II. The binding of alpha-amylase and beta-amylase to the starch granule.
During mango ripening, soluble sugars that account for mango sweetening are accumulated through carbon supplied by both photosynthesis and starch degradation. The cultivar Keitt has a characteristic dependence on sugar accumulation during starch degradation, which takes place during ripening, only a few days after detachment from the tree. Most knowledge about starch degradation is based on seeds and leaves currently used as models. However, information about the mango fruit is scarce. This work presents the evaluation of alpha- and beta-amylases in the starch granule surface during fruit development and ripening. Extractable proteins were assayed for amylase activity and detected by immunofluorescence microscopy and correlated to gene expression. The results suggest that both amylases are involved in starch degradation during mango ripening, probably under the dependence of another signal triggered by the detachment from the mother-plant.
Peroni
Fernanda Helena Gonçalves
FH
Laboratorio de Quimica, Bioquimica e Biologia Molecular de Alimentos, Departamento de Alimentos e Nutricao Experimental, FCF, Universidade de Sao Paulo, Avenida Lineu Prestes 580, Bloco 14, CEP 05508-900, Sao Paulo, SP, Brazil.
Koike
Claudia
C
Louro
Ricardo Pereira
RP
Purgatto
Eduardo
E
do Nascimento
João Roberto Oliveira
JR
Lajolo
Franco Maria
FM
Cordenunsi
Beatriz Rosana
BR
D003594:Cytoplasmic Granules / Q000201:enzymology; D005455:Fluorescent Antibody Technique; D005638:Fruit / Q000737:chemistry* / Q000254:growth & development / Q000648:ultrastructure; D031022:Mangifera / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D012333:RNA, Messenger / Q000032:analysis; D013213:Starch / Q000032:analysis / Q000378:metabolism*; D000516:alpha-Amylases / Q000235:genetics / Q000378:metabolism*; D001614:beta-Amylase / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D013213:Starch; D000516:alpha-Amylases; D001614:beta-Amylase