Soft lithography: masters on demand.
We report an ultra-rapid prototyping technique for forming microchannel networks for lab-on-a-chip applications, called masters on-demand. Channels are produced by replica molding on masters formed by laser printing on flexible copper printed circuit board (PCB) substrates. Masters of various designs and dimensions can be individually or mass produced in less than 10 minutes. Using this technique, we have fabricated channels as narrow as 100 microm with heights ranging between 9 microm and 70 microm. Multi-depth channel fabrication is also reported, using a two-step printing process. The functionality of devices formed in this manner is verified by performing in-channel electrophoretic separations and culture and analysis of primary mammalian cells.
Abdelgawad
Mohamed
M
Department of Mechanical and Industrial Engineering, University of Toronto, 5 King's College Road, Toronto, ON, CanadaM5S 3G8.
Watson
Michael W L
MW
Young
Edmond W K
EW
Mudrik
Jared M
JM
Ungrin
Mark D
MD
Wheeler
Aaron R
AR
D044085:Microfluidics / Q000191:economics / Q000295:instrumentation* / Q000379:methods*