A study on reconstruction of ossicular chain by an in situ bone tissue engineering technique.
CONCLUSIONS
The acellular cancellous bone with recombinant bone morphogenetic proteins 2 (rhBMP-2) could induce osteogenesis in the acoustic vesicle; this material might be used to reconstruct defects of the ossicular chain.


OBJECTIVE
In recent years, the in situ tissue engineering technique has improved rapidly, especially in bone regeneration. The aim of this study was to make an ossicular prosthesis using columnar acellular cancellous bone combined with rhBMP-2, implant this prosthesis into the acoustic vesicle of rabbits, and then observe the osteogenesis in situ.


METHODS
We prepared the acellular cancellous bone combined with rhBMP-2 as an ossicular prosthesis, while the same material without rhBMP-2 was used in the control group. From the retroauricular approach, we made a hole in the posterolateral bone wall of the acoustic vesicle, and the prepared materials were implanted. After 3 months, we observed the osteogenesis of the prosthesis by macroscopic anatomic and histologic methods.


RESULTS
The rabbits recovered soon after surgery. The implanted acellular cancellous bones were connected tightly with the bone of the acoustic vesicle wall. The surfaces of all materials were covered with mucosa, and osteogenesis was observed in the material with rhBMP-2.
Sun
Jian-Jun
JJ
Center of Otorhinolaryngology of PLA, Naval General Hospital, Beijing, China. jjsun85@sina.com.cn
Li
Xue-Sheng
XS
D000818:Animals; D001861:Bone Regeneration / Q000187:drug effects*; D003054:Cochlear Implants; D004429:Ear Ossicles / Q000378:metabolism*; D008562:Membrane Glycoproteins / Q000494:pharmacology*; D011817:Rabbits; D011994:Recombinant Proteins / Q000494:pharmacology; D013552:Swine; D023822:Tissue Engineering / Q000379:methods
C486403:EMP2 protein, human; D008562:Membrane Glycoproteins; D011994:Recombinant Proteins