Concentration control for protein crystallization via a continuously-fed crystallization chamber.
A continuously-fed crystallization chamber that allows for kinetic path control through the crystallization phase diagram (from labile/nucleation to metastable/growth) was fabricated and used to crystallize lysozyme. A lumped kinetic model was developed, and parameters for heterogeneous nucleation kinetics were determined. Heterogeneous nucleation was found to have faster nucleation kinetics and slower growth kinetics than homogeneous nucleation, as expected. The major contributions of the new device are (1) to allow better control of the chemical environment for studies of crystal nucleation and growth, and (2) to allow lumped-model analysis of those studies to extract kinetic parameters.
Sugiyama
Masano
M
Department of Chemical Engineering, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455, USA. sugiy002@umn.edu
Sengupta
Shramik
S
Todd
Paul
P
Barocas
Victor H
VH
D003460:Crystallization / Q000295:instrumentation* / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000737:chemistry*
D011506:Proteins