Quantitative analysis of heterogeneous spatial distribution of Arabidopsis leaf trichomes using micro X-ray computed tomography.
Quantitative morphological traits may be defined based on the 3D anatomy reconstructed from micro X-ray computed tomography (microCT) images. In this study, the heterogeneous spatial distribution of trichomes (hairs) on the adaxial leaf blade surface in Arabidopsis was evaluated in terms of 3D quantitative traits, including trichome number, average nearest-neighbour distance between trichomes, and proportion of large trichomes. The data reflect spatial heterogeneity in the radial direction, in that a greater number of trichomes were observed on the leaf blade margins relative to the non-margins, a distribution effect caused by the CAPRICE (CPC) and GLABRA3 (GL3) genes, which have previously been shown to affect trichome density. We further determined that the proportion of large trichomes on the blade mid-rib increases from the proximal end to the distal leaf tip in both wild-type plants and GL3 mutants. Our results indicate that the CPC [corrected] gene affects trichome distribution, rather than trichome growth, causing trichome initiation at the proximal base rather than the distal tip. On the other hand, CPC does affect trichome growth and developmental progression. Hence, quantitative phenotyping based on microCT enables precise phenotypic description for elucidation of gene control in morphological mutants.
Kaminuma
Eli
E
Bioinformatics and Systems Engineering Division, RIKEN Yokohama Institute, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Yoshizumi
Takeshi
T
Wada
Takuji
T
Matsui
Minami
M
Toyoda
Tetsuro
T
D000704:Analysis of Variance; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics; D029681:Arabidopsis Proteins / Q000235:genetics; D051792:Basic Helix-Loop-Helix Proteins / Q000235:genetics; D017343:Genes, Plant; D021621:Imaging, Three-Dimensional; D015233:Models, Statistical; D010641:Phenotype; D019441:Plant Epidermis / Q000166:cytology* / Q000235:genetics; D018515:Plant Leaves / Q000166:cytology* / Q000235:genetics; D030821:Plants, Genetically Modified / Q000166:cytology / Q000235:genetics; D020598:Proto-Oncogene Proteins c-myb / Q000235:genetics; D019655:Quantitative Trait, Heritable; D014057:Tomography, X-Ray Computed / Q000379:methods*
D029681:Arabidopsis Proteins; D051792:Basic Helix-Loop-Helix Proteins; D020598:Proto-Oncogene Proteins c-myb; C511989:CPC protein, Arabidopsis; C417284:GL3 protein, Arabidopsis