title: Human papillomavirus type 16 variants in cervical cancer from an admixtured population in Brazil.
abstract: Several studies indicate that molecular variants of HPV-16 have different geographic distribution and risk associated with persistent infection and development of high-grade cervical lesions. In the present study, the frequency of HPV-16 variants was determined in 81 biopsies from women with cervical intraepithelial neoplasia grade III or invasive cervical cancer from the city of Belem, Northern Brazil. Host DNAs were also genotyped in order to analyze the ethnicity-related distribution of these variants. Nine different HPV-16 LCR variants belonging to four phylogenetic branches were identified. Among these, two new isolates were characterized. The most prevalent HPV-16 variant detected was the Asian-American B-2, followed by the European B-12 and the European prototype. Infections by multiple variants were observed in both invasive cervical cancer and cervical intraepithelial neoplasia grade III cases. The analysis of a specific polymorphism within the E6 viral gene was performed in a subset of 76 isolates. The E6-350G polymorphism was significantly more frequent in Asian-American variants. The HPV-16 variability detected followed the same pattern of the genetic ancestry observed in Northern Brazil, with European, Amerindian and African roots. Although African ancestry was higher among women infected by the prototype, no correlation between ethnical origin and HPV-16 variants was found. These results corroborate previous data showing a high frequency of Asian-American variants in cervical neoplasia among women with multiethnic origin.
authors.affiliation: Department of Virology, Ludwig Institute for Cancer Research, São Paulo, Brazil.
authors.forename: Katiana
authors.initials: K
authors.lastname: Junes-Gill
authors.forename: Laura
authors.initials: L
authors.lastname: Sichero
authors.forename: Paulo Cesar
authors.initials: PC
authors.lastname: Maciag
authors.forename: Wyller
authors.initials: W
authors.lastname: Mello
authors.forename: Vânia
authors.initials: V
authors.lastname: Noronha
authors.forename: Luisa Lina
authors.initials: LL
authors.lastname: Villa
mesh_terms: D000328:Adult; D001706:Biopsy; D001938:Brazil / Q000453:epidemiology; D002584:Cervix Uteri / Q000821:virology; D004279:DNA, Viral / Q000235:genetics; D005006:Ethnicity; D005260:Female; D052162:Human papillomavirus 16 / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D008875:Middle Aged; D009856:Oncogene Proteins, Viral / Q000235:genetics; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D011110:Polymorphism, Genetic; D012091:Repetitive Sequences, Nucleic Acid; D012097:Repressor Proteins / Q000235:genetics; D002583:Uterine Cervical Neoplasms / Q000821:virology*
chemical_list: D004279:DNA, Viral; C068651:E6 protein, Human papillomavirus type 16; D009856:Oncogene Proteins, Viral; D012097:Repressor Proteins