title: A prostate cancer vaccine comprising whole cells secreting IL-7, effective against subcutaneous challenge, requires local GM-CSF for intra-prostatic efficacy.
abstract: A panel of cytokine-secreting RM-9 prostate cancer cells were tested as whole cell vaccines to determine their capacity to evoke an anti-prostate cancer immune response. In our model, vaccines secreting mGM-CSF or mIL-7 resulted in the highest increase in circulating T lymphocytes after vaccination, prolonged survival and, in a proportion of animals, tumor-free survival. Anti-tumor effects were more evident after a subcutaneous RM-9 challenge than after an intraprostatic challenge. However, when the RM-9/mGM-CSF cell line was used as intraprostatic tumor challenge, protection after RM-9/mIL-7 vaccination was restored.
authors.affiliation: Erasmus MC, Department of Urology, Josephine Nefkens Institute, Rotterdam, The Netherlands.
authors.forename: Caroline
authors.initials: C
authors.lastname: Schroten-Loef
authors.forename: Corrina M A
authors.initials: CM
authors.lastname: de Ridder
authors.forename: Suzanne
authors.initials: S
authors.lastname: Reneman
authors.forename: Marije
authors.initials: M
authors.lastname: Crezee
authors.forename: Angus
authors.initials: A
authors.lastname: Dalgleish
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Todryk
authors.forename: Chris H
authors.initials: CH
authors.lastname: Bangma
authors.forename: Robert
authors.initials: R
authors.lastname: Kraaij
mesh_terms: D000818:Animals; D019496:Cancer Vaccines / Q000627:therapeutic use*; D045744:Cell Line, Tumor; D016207:Cytokines / Q000378:metabolism; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism*; D015851:Interleukin-7 / Q000378:metabolism*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008954:Models, Biological; D011471:Prostatic Neoplasms / Q000276:immunology* / Q000628:therapy*; D013601:T-Lymphocytes / Q000378:metabolism; D013997:Time Factors; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D019496:Cancer Vaccines; D016207:Cytokines; D015851:Interleukin-7; D016159:Tumor Suppressor Protein p53; D016178:Granulocyte-Macrophage Colony-Stimulating Factor