[Synthesis of Sudan I artificial antigen].
OBJECTIVE
To prepare Sudan I-HS conjugates with the carrier protein for developing a new immune assay for Sudan I.


METHODS
Sudan I was actived by succinic anhydride and converted into Sudan I hemisuccinate (Sudan I-HS) as the hapten, then Sudan I-HS was linked to carrier protein bovine serum albumin (BSA) as immunogen, and ovalbumin (OVA) as coating antigen all by the active ester method.


RESULTS
The results of IR and HPLC-MS indicated that the hapten was synthesized successfully. The conjugation ratios of Sudan I-HS-BSA and Sudan I-HS-OVA confirmed by ultraviolet spectrum were 11 : 1 and 5 : 1, respectively.


CONCLUSIONS
Antigens Sudan I-HS-BSA and Sudan I-HS-OVA were prepared successfully.
Yu
Wei
W
College of Food Science and Technology, Huazhong Agricultural University, Key Lab Food Security Estimate of Agriculture, Wuhan 430070, China. wudichina2001@yahoo.com.cn
Wang
Xiaohong
X
Zhou
Youxiang
Y
Wang
Ping
P
D000941:Antigens / Q000276:immunology*; D002273:Carcinogens / Q000032:analysis; D004396:Coloring Agents / Q000032:analysis*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005504:Food Analysis / Q000379:methods; D009284:Naphthols / Q000138:chemical synthesis* / Q000276:immunology*; D012710:Serum Albumin, Bovine / Q000737:chemistry
D000941:Antigens; D002273:Carcinogens; D004396:Coloring Agents; D009284:Naphthols; D012710:Serum Albumin, Bovine; C024336:1-phenylazo-2-naphthol