[Sulfated modification conditions optimization of astragalus polysaccharide by orthogonal test and anti-IBDV activity determination of the modifiers].
OBJECTIVE
To optimize the sulfated modification conditions of astragalus polysaccharide (APS) and probe into the probability of sulfated modification to enhance APS activity.


METHODS
Total APS extracted by one-step ethanol precipitation and three fractional APSs extracted by stepwise ethanol precipitation method and purified were modified by chlorosulfonic acid-pyridine method. The modification conditions were optimized by L9 (3(4)) orthogonal design taking the ratio of chlorosulfonic acid to pyridine, reaction temperature and time. The degree of substitution (DS) was tested by sulfate barium turbidimetric method. The anti-IBDV activity of modification production was tested by MTT method.


RESULTS
The optimized modification conditions were 1 : 6 of chlorosulfonic acid to pyridine, reaction temperature of 95 degrees C and reaction time of 1 hour.


CONCLUSIONS
Sulfated modification can enhance the antiviral activity of APS, which related to DS.
Huang
Xiao-Yan
XY
Institute of Traditional Chinese Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China.
Hu
Yuan-Liang
YL
Lu
Yu
Y
Zhang
Fan
F
Guo
Zhen-Huan
ZH
D000704:Analysis of Variance; D000818:Animals; D000998:Antiviral Agents / Q000737:chemistry* / Q000494:pharmacology; D027883:Astragalus Plant / Q000737:chemistry*; D002645:Chickens; D005240:Feasibility Studies; D007243:Infectious bursal disease virus / Q000187:drug effects; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology; D013463:Sulfuric Acid Esters / Q000737:chemistry* / Q000378:metabolism; D013696:Temperature; D013997:Time Factors
D000998:Antiviral Agents; D011134:Polysaccharides; D013463:Sulfuric Acid Esters