Optical microscopy study of human sclera stored in different media.
OBJECTIVE
To describe microscopic changes in the structure of human sclera immediately after enucleation (negative control group) and to compare them after being stored for three months in four different media: pure glycerin, absolute alcohol, benzalkonium chloride diluted in absolute alcohol (1:5000), and benzalkonium chloride diluted in balanced salt solution (1:5000). The comparison took into consideration their final state of conservation.


METHODS
Experimental study, laboratory investigation.


METHODS
Optical microscopy was used to study the specimens after they had been in storage for one, two, and three months. The scleral fragments were prepared in thin plates, dyed, and then submitted to histologic analysis by two masked specialists. Unpreserved scleral fragments obtained right after enucleation were assessed and served as negative controls.


RESULTS
The collagen fibers of scleras stored in glycerin presented with a more regular pattern, closer in appearance to the negative control group. Scleras stored in the other three media presented contorted collagen fibers.


CONCLUSIONS
Our findings suggest that glycerin is close to being the ideal storage medium for sclera, because it maintains the structural characteristics of the collagen fibers. Research is ongoing to determine how to increase the bactericidal and antiviral properties of glycerin storage.
Bernardini Roriz
Maria Cristina P
MC
Department of Ophthalmology, Federal University of Goiás, Rua Rui Brasil Cavalcante 229, Setor Oeste, Goiânia, Goiás, Brazil. mcpbernardini@brturbo.com.br
Pereira de Avila
Marcos
M
Barbosa De Sousa
Luciene
L
D000085:Acetates / Q000494:pharmacology*; D001548:Benzalkonium Compounds / Q000494:pharmacology; D003094:Collagen / Q000187:drug effects; D004338:Drug Combinations; D000431:Ethanol / Q000494:pharmacology*; D015353:Eye Enucleation; D005990:Glycerol / Q000494:pharmacology*; D006801:Humans; D008853:Microscopy; D008903:Minerals / Q000494:pharmacology*; D009926:Organ Preservation / Q000379:methods*; D011310:Preservatives, Pharmaceutical / Q000494:pharmacology; D012590:Sclera / Q000187:drug effects / Q000473:pathology*; D012965:Sodium Chloride / Q000494:pharmacology*; D013696:Temperature
D000085:Acetates; C030660:BSS solution; D001548:Benzalkonium Compounds; D004338:Drug Combinations; D008903:Minerals; D011310:Preservatives, Pharmaceutical; D000431:Ethanol; D012965:Sodium Chloride; D003094:Collagen; D005990:Glycerol