Development and validation of a gas chromatographic-mass spectrometric method for quantitative determination of perphenazine in rabbit plasma after sublingual administration.
Perphenazine is a phenothiazine-type antipsychotic that is a potential candidate for sublingual administration due to its extensive first-pass metabolism. In this study, a gas chromatographic-mass spectrometric method was developed for quantification of perphenazine in rabbit plasma after sublingual administration. The plasma samples were purified by mixed-mode solid phase extraction with good recovery (>83%). The method was linear (r(2)>0.99) over a range of 2-64 ng/ml, with a lower limit of quantification of 2 ng/ml. The accuracy was 100+/-4%, and the within-day and between-day precisions were <6.8% R.S.D. and <14% R.S.D., respectively. Perphenazine was stable in stock solutions and plasma. The method was successfully applied for analysing perphenazine in plasma after sublingual administration to rabbits.
Turunen
Elina
E
Department of Pharmaceutical Chemistry, University of Kuopio, P.O. Box 1627, FIN-70211, Kuopio, Finland. elina.turunen@uku.fi
Lehtonen
Marko
M
Järvinen
Tomi
T
Jarho
Pekka
P
D000286:Administration, Sublingual; D000818:Animals; D014150:Antipsychotic Agents / Q000008:administration & dosage / Q000097:blood*; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D010546:Perphenazine / Q000008:administration & dosage / Q000097:blood*; D011817:Rabbits; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D014150:Antipsychotic Agents; D010546:Perphenazine