title: Genomic copy number alterations as predictive markers of systemic recurrence in breast cancer.
abstract: We tried to establish models that predict systemic recurrence in breast cancer by selecting marker clones with DNA copy number alterations (CNAs) using an array comparative genomic hybridization (CGH). Array CGH containing 4,044 human bacterial artificial chromosome clones was used to assess CNAs in 62 primary breast cancer tissues from 31 patients with systemic recurrence within 5 years after surgery and clinicopathologically well matched 31 patients who had no evidence of disease for at least 5years. Fourteen significant clones (11 clones showing gain and 3 showing loss) were identified by systemic recurrence-free survival (SRFS) analysis and 23 significant clones (17 clones showing gain and 6 showing loss) identified by chi(2) test and FDR test were selected as predictive markers of systemic breast cancer recurrence. The significant CNAs were found in the chromosomal regions of 5p15.33, 11q13.3, 15q26.3, 17q25.3, 18q23 and 21q22.3 with gain and 9p12, 11q24.1 and 14q32.33 with loss. We devised 2 prediction models for the systemic recurrence of breast cancer based on the 14 clones and the 23 clones, respectively. The survivals of the patients were significantly separated according to the scores from each model at the optimal cut off values in SRFS and overall survival analysis. We found candidate clones and genes of which CNAs were significantly associated with systemic recurrence of breast cancer. The devised prediction models with these clones were effective at differentiating the recurrence and nonrecurrence.
authors.affiliation: Department of Surgery, Seoul National University Boramae Hospital, Seoul, South Korea.
authors.forename: Ki-Tae
authors.initials: KT
authors.lastname: Hwang
authors.forename: Wonshik
authors.initials: W
authors.lastname: Han
authors.forename: Jihyoung
authors.initials: J
authors.lastname: Cho
authors.forename: Jong Won
authors.initials: JW
authors.lastname: Lee
authors.forename: Eunyoung
authors.initials: E
authors.lastname: Ko
authors.forename: Eun Kyu
authors.initials: EK
authors.lastname: Kim
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Jung
authors.forename: Eun-Mi
authors.initials: EM
authors.lastname: Jeong
authors.forename: Ji-Yeon
authors.initials: JY
authors.lastname: Bae
authors.forename: Jason J
authors.initials: JJ
authors.lastname: Kang
authors.forename: Song-Ju
authors.initials: SJ
authors.lastname: Yang
authors.forename: Sung-Won
authors.initials: SW
authors.lastname: Kim
authors.forename: Dong-Young
authors.initials: DY
authors.lastname: Noh
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D016000:Cluster Analysis; D004273:DNA, Neoplasm / Q000032:analysis / Q000235:genetics; D005260:Female; D018628:Gene Dosage; D020022:Genetic Predisposition to Disease; D006801:Humans; D008875:Middle Aged; D009364:Neoplasm Recurrence, Local / Q000235:genetics* / Q000473:pathology; D009693:Nucleic Acid Hybridization; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests
chemical_list: D014408:Biomarkers, Tumor; D004273:DNA, Neoplasm