[Isolation and identification of toxins inhibiting Dentrolimus tabulaeformis from an antagonistic strain of Beauveria].
OBJECTIVE
We used submerged fermentation to cultivate a strain of the entomopathogenic fungus Beauveria tenella isolated from the infected larvae of Dentrolimus tabulaeformis in Pinus tabulaeformis forest in Chengde of Hebei Province in China.


METHODS
We used ethyl acetate to extract antagonistic components from the fermentation broth and used silica gel column chromatography and GC/MS to separate and identify the components.


RESULTS
Six compounds were obtained by silica gel column chromatography. The sixth compound had higher activity to kill the larvae of Dentrolimus tabulaeformis with a corrected mortality rate of 80%. Seventeen compounds were separated and identified by GC/MS in the 6th group, of which 3compounds were more than 10%, 2-Piperidinone (14.02%), 2-coumaranone (47.10%), and Pyrrolo[1,2-a]Pyrazine-1,4-dione, hexahydro (21.05%).


CONCLUSIONS
2-Piperidinone and 2-coumaranone had insecticidal activity (corrected mortality rate reached 83.32% and 91.61% respectively) and were the most important toxic substances to control pests.
Fan
Jinhua
J
School of Life Science and Technology, Shanxi University, Taiyuan 030006, China. fjhlbz@yahoo.com.cn
Xie
Yingping
Y
Xue
Jiaoliang
J
Li
Baozhen
B
D000818:Animals; D052982:Beauveria / Q000737:chemistry* / Q000502:physiology*; D002850:Chromatography, Gel; D008401:Gas Chromatography-Mass Spectrometry; D007814:Larva / Q000187:drug effects; D007915:Lepidoptera / Q000187:drug effects*; D009183:Mycotoxins / Q000032:analysis* / Q000302:isolation & purification* / Q000633:toxicity
D009183:Mycotoxins