Environmentally relevant concentrations of 17alpha-ethinylestradiol (EE2) interfere with the growth hormone (GH)/insulin-like growth factor (IGF)-I system in developing bony fish.
The aim of this study was to evaluate whether effects of environmental estrogens on fish growth and reproduction may be mediated via modulating the growth hormone (GH)/insulin-like growth factor I (IGF-I) system. To this end, developing male and female monosex populations of tilapia were exposed to 17alpha-ethinylestradiol (EE2) at 5 and 25 ng EE2/l water from 10-day postfertilization (DPF) until 100 DPF. Under exposure to both EE2 concentrations, sex ratio shifted toward more females and body length, and weight were significantly reduced in males. The growth-reducing effect was associated with significant changes in hepatic IGF-I expression, both in males and females and with significant alterations of IGF-I mRNA and GH mRNA in the brain. The changes in IGF-I and GH mRNA were accompanied by altered estrogen receptor alpha (ERalpha) expression in brain and liver. These findings point to an influence of estrogenic exposure on the endocrine GH/IGF-I axis. In addition, the EE2 treatment resulted in significant changes of ERalpha and IGF-I expression in ovaries and testis, suggesting that the estrogens interact not only with the endocrine but also with the autocrine/paracrine part of the IGF-I system. Overall, our results provide evidence that EE2 at environmentally relevant concentrations is able to interfere with the GH/IGF-I system in bony fish and that the impairing effects of estrogens reported on fish growth and reproductive functions may rather result from a cross talk between the sex steroid and the IGF-I system than be toxicological.
Shved
Natallia
N
Division of Neuroendocrinology, Institute of Anatomy, University of Zürich, Zürich, Switzerland.
Berishvili
Giorgi
G
Baroiller
Jean-François
JF
Segner
Helmut
H
Reinecke
Manfred
M
D000818:Animals; D049628:Body Size / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D052244:Endocrine Disruptors / Q000633:toxicity*; D047628:Estrogen Receptor alpha / Q000378:metabolism; D004997:Ethinyl Estradiol / Q000633:toxicity*; D005260:Female; D029941:Fish Proteins / Q000235:genetics / Q000378:metabolism*; D013006:Growth Hormone / Q000235:genetics / Q000378:metabolism*; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D008297:Male; D010053:Ovary / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D010902:Pituitary Gland / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D012744:Sex Ratio; D013737:Testis / Q000187:drug effects / Q000196:embryology / Q000378:metabolism; D017210:Tilapia / Q000196:embryology / Q000378:metabolism*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
D052244:Endocrine Disruptors; D047628:Estrogen Receptor alpha; D029941:Fish Proteins; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D004997:Ethinyl Estradiol; D007334:Insulin-Like Growth Factor I; D013006:Growth Hormone