Expression of CgCDR1, CgCDR2, and CgERG11 in Candida glabrata biofilms formed by bloodstream isolates.
Many studies have shown that Candida biofilms are highly resistant to fluconazole. The mechanisms of acquired fluconazole resistance of Candida glabrata in non-biofilms have been elucidated, but the relevance of them relative to C. glabrata in biofilms are unknown. We examined by real-time PCR the expression of CgCDR1, CgCDR2, and CgERG11 of four bloodstream isolates of C. glabrata during the early (6 h), intermediate (15 h), and mature (48 h) phases of biofilm development. We found high levels of biofilm formation in vitro. At 6 and 15 h, the biofilms exhibited, in comparison to planktonic cells, approximately 1.5- and 3.3-fold upregulation of CgCDR1 and 0.5- and 3.1-fold upregulation of CgCDR2, respectively. However, at 48 h, neither gene was upregulated. In comparison, the expression of CgERG11 did not significantly increase during any of the three phases. Our results indicate a temporary increase in the expression of both CgCDR1 and CgCDR2 during the intermediate phase of C. glabrata biofilm development. In addition, the data collected at 48 h suggest that CgCDR1, CgCDR2, and CgERG11 may not play a role in the azole resistance of C. glabrata in mature biofilms.
Song
Jeong Won
JW
Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea.
Shin
Jong Hee
JH
Kee
Seung Jung
SJ
Kim
Soo Hyun
SH
Shin
Myung Geun
MG
Suh
Soon Pal
SP
Ryang
Dong Wook
DW
D018441:Biofilms; D041221:Candida glabrata / Q000302:isolation & purification / Q000378:metabolism / Q000502:physiology*; D002177:Candidiasis / Q000382:microbiology; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D016469:Fungemia / Q000382:microbiology*; D006801:Humans; D026901:Membrane Transport Proteins / Q000235:genetics* / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D012331:RNA, Fungal / Q000032:analysis
D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D012331:RNA, Fungal