Fabrication of collagen hybridized elastic PLCL for tissue engineering.
Biodegradable elastic poly(L-lactide-co-epsilon-caprolactone) (PLCL) (50:50) copolymer was blended with collagen (0.05, 0.1 and 0.2% w/w) in an acidic dioxane solution to form a collagen/PLCL hybrid material suitable for tissue engineering applications. Stability and dispersivity of collagen on collagen/PLCL hybrid films and collagen coated PLCL films under mechanical stress were determined by a collagen release test and water contact angle measurement. Hybrid films had a higher stability than collagen-coated PLCL films. Elastic recovery as well as high interconnectivity and uniform pore morphology of the hybrid scaffolds were not affected by the collagen concentration. Fibroblasts (NIH-3T3) cell culture test was performed for cell growth and viability evaluation. Collagen concentration had little affect on the initial cell adhesion after 4 h cell culture; but after 48 h cell culture, increased cell proliferation on the hybrid films was observed. The hybrid material can be applied as a scaffold for vessel and cartilage regeneration for mechano-active tissue engineering.
Lim
Jin Ik
JI
Department of Dental Biomaterials Science, Dental Research Institute and BK21 Program, School of Dentistry, Seoul National University, 28 Yeongeon-dong, Jongro-gu, Seoul 110-749, Republic of Korea.
Yu
Bin
B
Lee
Yong-Keun
YK
D000818:Animals; D002448:Cell Adhesion; D003094:Collagen / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D011091:Polyesters / Q000138:chemical synthesis / Q000737:chemistry*; D011137:Polystyrenes; D013314:Stress, Mechanical; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
D011091:Polyesters; D011137:Polystyrenes; C062968:lactide-caprolactone copolymer; D003094:Collagen