Influence of bacterial superantigen TSST-1 against the anti-proliferative efficacy of immunosuppressive drugs and interleukin 2 production in peripheral blood mononuclear cells of hemodialysis patients and healthy subjects.
We investigated the influence of bacterial superantigen on the efficacies of immunosuppressive drugs on the blastogenesis of peripheral-blood mononuclear cells of 27 hemodialysis patients awaiting renal transplantation. The IC(50) values for prednisolone, methylprednisolone, cyclosporine, and tacrolimus evaluated in the superantigen-stimulated cells were significantly higher than those evaluated in concanavalin A-stimulated cells (p = 0.0002-0.018). Interleukin-2 amounts produced from superantigen-stimulated cells were significantly larger than those from concanavalin A-stimulated cells (p = 0.0363). These results suggest that superantigen attenuates the suppressive efficacies of glucocorticoids and calcineurin inhibitors by stimulating lymphocytes of hemodialysis patients awaiting transplantation to overproduce interleukin-2.
Taira
Shinichiro
S
Department of 5th Surgery, Hachioji Medical Center, Tokyo Medical University, Tokyo, Japan.
Katsuyama
Kanako
K
Konno
Osamu
O
Ashizawa
Tatsuto
T
Matsuno
Naoto
N
Nagao
Takeshi
T
Hirano
Toshihiko
T
D000328:Adult; D000368:Aged; D001427:Bacterial Toxins; D002478:Cells, Cultured; D004306:Dose-Response Relationship, Immunologic; D004768:Enterotoxins / Q000502:physiology*; D005260:Female; D006131:Growth Inhibitors / Q000037:antagonists & inhibitors / Q000502:physiology*; D006801:Humans; D007166:Immunosuppressive Agents / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D007376:Interleukin-2 / Q000096:biosynthesis* / Q000502:physiology; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D006435:Renal Dialysis; D018089:Superantigens / Q000502:physiology*; D055815:Young Adult
D001427:Bacterial Toxins; D004768:Enterotoxins; D006131:Growth Inhibitors; D007166:Immunosuppressive Agents; D007376:Interleukin-2; D018089:Superantigens; C030107:enterotoxin F, Staphylococcal