Extracellular proteases from the Antarctic marine pseudoalteromonas sp. P96-47 strain.
The extracellular protease-production capacity of 33 bacterial isolates taken from marine biotopes in King George Island, Antarctica, was evaluated in liquid cultures. The P96-47 isolate was selected due to its high production capacity and was identified as Pseudoalteromonas sp. The optimal growth temperature was 20 degrees C and the optimal for protease production was 15 degrees C. Proteases were purified from culture supernatants, developing a multiple-band profile in zymograms. They were classified as neutral metalloproteases and worked optimally at 45 degrees C with an Eact of 47 kJ/mol. Their stability was higher at neutral pH, retaining more than 80% of activity at pH 6-10 after 3 h incubation at 4 degrees C. After 90 min incubation at 40 and 50 degrees C, the percentages of residual activities were 78% and 44%. These results contribute to the basic knowledge of Antarctic marine proteases and also help evaluate the probable industrial applications of P96-47 proteases.
Vázquez
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SC
Cátedra de Microbiología Industrial y Biotecnología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 60 Piso (C 113AAD) Ciudad Autónoma de Buenos Aires, Argentina. svazquez@ffyb.uba.ar
Hernández
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Mac Cormack
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WP
D010447:Peptide Hydrolases / Q000096:biosynthesis*; D031161:Pseudoalteromonas / Q000201:enzymology*; D012623:Seawater / Q000382:microbiology; D014871:Water Microbiology
D010447:Peptide Hydrolases