title: Nestin is expressed in basal-like and triple negative breast cancers.
abstract: OBJECTIVE
  To analyse the distribution of nestin expression in different breast tumours and to determine the prognostic impact of nestin expression.
  
  
  METHODS
  Nestin expression was immunohistochemically analysed in a cohort of 245 invasive breast cancer patients treated with therapeutic surgery followed by anthracycline-based chemotherapy using a semi-quantitative scoring system.
  
  
  RESULTS
  Nestin was exclusively expressed in grade III breast carcinoma and preferentially expressed in basal-like and triple negative cancers. Nestin-positive tumours displayed high proliferation rates and p53 nuclear expression. Lymph-node positive patients with nestin-positive cancers had a shorter breast cancer specific survival; however nestin was not an independent prognostic factor on multivariate analysis.
  
  
  CONCLUSIONS
  Nestin expression is preferentially found in basal-like and triple negative breast carcinomas. Further studies are warranted to define the biological role played by nestin in these subgroups of breast cancers.
authors.affiliation: Molecular Pathology Laboratory, The Breakthrough Breast Cancer Research Centre, Institute of Cancer Research, London, UK.
authors.forename: S
authors.initials: S
authors.lastname: Parry
authors.forename: K
authors.initials: K
authors.lastname: Savage
authors.forename: C
authors.initials: C
authors.lastname: Marchiò
authors.forename: J S
authors.initials: JS
authors.lastname: Reis-Filho
mesh_terms: D000230:Adenocarcinoma / Q000737:chemistry* / Q000188:drug therapy / Q000601:surgery; D018943:Anthracyclines / Q000627:therapeutic use; D000970:Antineoplastic Agents / Q000627:therapeutic use; D001943:Breast Neoplasms / Q000737:chemistry* / Q000188:drug therapy / Q000601:surgery; D002277:Carcinoma / Q000737:chemistry / Q000188:drug therapy / Q000601:surgery; D018270:Carcinoma, Ductal, Breast / Q000737:chemistry / Q000188:drug therapy / Q000601:surgery; D018275:Carcinoma, Lobular / Q000737:chemistry / Q000188:drug therapy / Q000601:surgery; D017024:Chemotherapy, Adjuvant; D005260:Female; D005500:Follow-Up Studies; D015870:Gene Expression; D016158:Genes, p53; D006801:Humans; D007150:Immunohistochemistry; D009363:Neoplasm Proteins / Q000032:analysis*; D064231:Nestin / Q000032:analysis*; D011379:Prognosis; D040081:Protein Array Analysis
chemical_list: D018943:Anthracyclines; D000970:Antineoplastic Agents; D009363:Neoplasm Proteins; D064231:Nestin