title: Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery.
abstract: A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.
authors.affiliation: Department of Biochemistry, Yonsei Proteome Research Center and Biomedical Proteome Research Center, Yonsei University, Seoul, Korea.
authors.forename: Hyoung-Joo
authors.initials: HJ
authors.lastname: Lee
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Kang
authors.forename: Eun-Young
authors.initials: EY
authors.lastname: Lee
authors.forename: Sang Yun
authors.initials: SY
authors.lastname: Cho
authors.forename: Hoguen
authors.initials: H
authors.lastname: Kim
authors.forename: Young-Ki
authors.initials: YK
authors.lastname: Paik
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis* / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
chemical_list: D015415:Biomarkers; D010455:Peptides; D000469:Alkaline Phosphatase