title: Carbon nanotubes in macrophages: imaging and chemical analysis by X-ray fluorescence microscopy.
abstract: X-ray fluorescence microscopy (microXRF) is applied for the first time to study macrophages exposed to unpurified and purified single-walled (SW) and multiwalled (MW) carbon nanotubes (CNT). Investigating chemical elemental distributions allows one to (i) image nanotube localization within a cell and (ii) detect chemical modification of the cell after CNT internalization. An excess of calcium is detected for cells exposed to unpurified SWCNT and MWCNT and related toxicological assays are discussed.
authors.affiliation: Laboratoire de Physique des Solides, UMR 8502 CNRS, Universite Paris Sud, Bat. 510, Orsay F-91405, France. bussy@lps.u-psud.fr
authors.forename: Cyrill
authors.initials: C
authors.lastname: Bussy
authors.forename: Julien
authors.initials: J
authors.lastname: Cambedouzou
authors.forename: Sophie
authors.initials: S
authors.lastname: Lanone
authors.forename: Emilie
authors.initials: E
authors.lastname: Leccia
authors.forename: Vasile
authors.initials: V
authors.lastname: Heresanu
authors.forename: Mathieu
authors.initials: M
authors.lastname: Pinault
authors.forename: Martine
authors.initials: M
authors.lastname: Mayne-L'hermite
authors.forename: Nathalie
authors.initials: N
authors.lastname: Brun
authors.forename: Claudie
authors.initials: C
authors.lastname: Mory
authors.forename: Marine
authors.initials: M
authors.lastname: Cotte
authors.forename: Jean
authors.initials: J
authors.lastname: Doucet
authors.forename: Jorge
authors.initials: J
authors.lastname: Boczkowski
authors.forename: Pascale
authors.initials: P
authors.lastname: Launois