title: Comparison of laboratory-based and phylogenetic methods to distinguish between Haemophilus influenzae and H. haemolyticus.
abstract: New methods to distinguish between nontypeable Haemophilus influenzae and nonhemolytic H. haemolyticus were compared. The results of iga variable region hybridization to dotblots and library-on-a-slide microarrays were more similar to a "gold standard" multigenephylogenetic tree than iga-conserved region hybridization or P6 7F3 epitope immunoblots.
authors.affiliation: Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, Michigan 48109-2029, United States. sandsted@umich.edu
authors.forename: Sara A
authors.initials: SA
authors.lastname: Sandstedt
authors.forename: Lixin
authors.initials: L
authors.lastname: Zhang
authors.forename: Mayurika
authors.initials: M
authors.lastname: Patel
authors.forename: Kirk W
authors.initials: KW
authors.lastname: McCrea
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Qin
authors.forename: Carl F
authors.initials: CF
authors.lastname: Marrs
authors.forename: Janet R
authors.initials: JR
authors.lastname: Gilsdorf
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry; D002353:Carrier State / Q000382:microbiology*; D002675:Child, Preschool; D000939:Epitopes / Q000032:analysis; D006190:Haemophilus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D018073:Haemophilus Vaccines / Q000737:chemistry; D006193:Haemophilus influenzae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D015151:Immunoblotting / Q000379:methods*; D009693:Nucleic Acid Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010614:Pharynx / Q000382:microbiology; D010802:Phylogeny; D012697:Serine Endopeptidases / Q000235:genetics*
chemical_list: D001425:Bacterial Outer Membrane Proteins; D000939:Epitopes; D018073:Haemophilus Vaccines; C069990:P6 outer membrane protein, Haemophilus; D012697:Serine Endopeptidases; C021433:IgA-specific serine endopeptidase