Brain-derived neurotrophic factor gene expression in pediatric bipolar disorder: effects of treatment and clinical response.
OBJECTIVE
Pediatric bipolar disorder (PBD) is a major public health concern; however, little is known about the cellular and genetic factors that are involved in the pathophysiology of this illness. The observed structural abnormality in the brains of patients with mood disorders has been related to abnormal brain-derived neurotrophic factor (BDNF) function, suggesting an important role for BDNF in these disorders.


METHODS
We determined the gene expression of BDNF in lymphocytes obtained from 26 PBD subjects during a drug-free baseline period and during the eighth week of treatment (n = 19) and from 21 medication-free normal control subjects. We also determined the protein levels of BDNF in platelets of patients with PBD and normal control subjects. Subjects were diagnosed according to DSM-IV diagnostic criteria using the Washington University at St. Louis Schedule for Affective Disorders and Schizophrenia.


RESULTS
The mRNA levels of BDNF in lymphocytes of PBD subjects were significantly decreased compared with those of normal control subjects and were significantly higher in 19 subjects after 8 weeks of treatment than the pretreatment drug-free baseline levels and similar to those of normal controls. Similarly, protein levels of BDNF were decreased in platelets of patients with PBD.


CONCLUSIONS
These studies suggest that BDNF levels may be a potential biomarker for PBD. BDNF levels may also serve as a potential treatment predictor and prognostic indicator in PBD.
Pandey
Ghanshyam N
GN
Department of Psychiatry, University of Illinois at Chicago, Chicago, IL 60612, USA. Gnpandey@psych.uic.edu
Rizavi
Hooriyah S
HS
Dwivedi
Yogesh
Y
Pavuluri
Mani N
MN
D000293:Adolescent; D000927:Anticonvulsants / Q000627:therapeutic use; D018692:Antimanic Agents / Q000627:therapeutic use; D014150:Antipsychotic Agents / Q000627:therapeutic use; D001714:Bipolar Disorder / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics*; D001792:Blood Platelets / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000235:genetics*; D002648:Child; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000378:metabolism; D008297:Male; D011379:Prognosis; D011569:Psychiatric Status Rating Scales; D012333:RNA, Messenger / Q000097:blood; D020133:Reverse Transcriptase Polymerase Chain Reaction
D000927:Anticonvulsants; D018692:Antimanic Agents; D014150:Antipsychotic Agents; D019208:Brain-Derived Neurotrophic Factor; D012333:RNA, Messenger