Study of optical properties of Macrophomina phaseolina impregnated sol-gel derived silica matrices.
In the present frame of work, Macrophomina phaseolina is encapsulated in silica matrices at various concentrations by low temperature sol-gel technique using tetraethylorthosilicate (TEOS) as precursor. The optical and photophysical properties of these samples have been studied by second harmonics of Nd:YAG laser at 532 nm. UV-visible absorption spectra of samples have been recorded and it is found that the absorption increases with increase in concentration of fungus. Further, a decrease in output transmission intensity of the laser has been observed with increase in fungus concentration. The temporal response of these samples has also been examined. The results show that the fungus concentration can be measured within approximately 15-20 min. This method of optical sensing of fungus in test sample is faster than other techniques, such as the conventional colorimetric method which takes about 1 h.
Sharma
Sunita
S
Department of Applied Physics, Guru Jambheshwar University of Science and Technology, Hisar 125001 Haryana, India. sunphotonics@gmail.com
Vandana
Ghoshal
S K
SK
Arora
Pooja
P
Dilbaghi
Neeraj
N
Chaudhury
Ashok
A
D001203:Ascomycota / Q000302:isolation & purification* / Q000502:physiology*; D001672:Biocompatible Materials / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation*; D015169:Colony Count, Microbial / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D008027:Light; D055096:Optical Devices; D044367:Phase Transition; D012031:Refractometry / Q000295:instrumentation*; D015203:Reproducibility of Results; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D013048:Specimen Handling / Q000379:methods
D001672:Biocompatible Materials; D012822:Silicon Dioxide