Pan-dengue virus detection by PCR for travelers returning from the tropics.
We performed dengue virus (DENV) serology and quantitative real-time pan-DENV reverse transcription-PCR (RT-PCR) on 186 sera of 171 patients returning from the tropics. DENV loads significantly decreased with increasing times of disease and were higher in immunoglobulin M-negative samples. In the first week of disease, pan-DENV RT-PCR is the test of choice.
Dumoulin
Alexis
A
Department of Biomedicine, Division of Diagnostics, Institute for Medical Microbiology, University of Basel, Basel, Switzerland.
Marti
Hanspeter
H
Panning
Marcus
M
Hatz
Christoph
C
Hirsch
Hans H
HH
D000328:Adult; D001210:Asia, Southeastern; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013557:Switzerland; D014195:Travel