title: Trafficking of cGMP-dependent protein kinase II via interaction with Rab11.
abstract: cGMP-dependent protein kinase II (cGK-II) is implicated in several physiological functions including intestinal secretion, bone growth, and learning and memory, but the detailed mechanisms are still unclear. To identify proteins that are involved in cGMP/cGK-II signaling, we performed yeast two-hybrid screening and identified Rab11b as a cGK-II-interacting protein that regulates the slow-recycling pathway. Interestingly, cGK-II interacted with the GDP-bound form of Rab11b (Rab11b S25N), but not the GTP-bound form, in mammalian cells. Immunofluorescence staining revealed that Rab11b S25N promoted the translocation of cGK-II from the plasma membrane to the cytoplasm and that the localization of cGK-II extensively overlapped with Rab11b. Furthermore, treatment with a membrane-permeable cGMP analog caused the rapid retranslocation of cGK-II and Rab11b S25N to the membrane. These data indicate that Rab11b is necessary for the trafficking of cGK-II and that the cGMP/cGK-II signaling pathway is closely related to Rab11b recycling pathway.
authors.affiliation: Department of Biological Science and Technology, The University of Tokushima Graduate School, Tokushima 770-8506, Japan. yuasa@bio.tokushima-u.ac.jp
authors.forename: Keizo
authors.initials: K
authors.lastname: Yuasa
authors.forename: Shin
authors.initials: S
authors.lastname: Yamagami
authors.forename: Masami
authors.initials: M
authors.lastname: Nagahama
authors.forename: Akihiko
authors.initials: A
authors.lastname: Tsuji
mesh_terms: D000818:Animals; D002460:Cell Line; D002462:Cell Membrane / Q000201:enzymology; D062967:Cyclic GMP-Dependent Protein Kinase Type II; D017869:Cyclic GMP-Dependent Protein Kinases / Q000037:antagonists & inhibitors / Q000378:metabolism*; D006150:Guanine Nucleotides / Q000378:metabolism / Q000494:pharmacology; D006801:Humans; D051379:Mice; D021381:Protein Transport; D015398:Signal Transduction; D020798:Two-Hybrid System Techniques; D020691:rab GTP-Binding Proteins / Q000378:metabolism*; D000099193:rab11 GTP-Binding Proteins
chemical_list: D062967:Cyclic GMP-Dependent Protein Kinase Type II; D017869:Cyclic GMP-Dependent Protein Kinases; D006150:Guanine Nucleotides; D020691:rab GTP-Binding Proteins; D000099193:rab11 GTP-Binding Proteins; C569729:PRKG2 protein, human; C569730:Prkg2 protein, mouse