title: A PstI polymorphism at 3'UTR of goat POU1F1 gene and its effect on cashmere production.
abstract: POU1F1 is a positive regulator for prolactin (PRL) whose metabolites may directly or indirectly affect some aspects of the hair growth cycle, therefore, POU1F1 gene is an important candidate gene for cashmere traits selection through marker-assisted selection (MAS). Hence, in this study, the PCR-RFLP method was applied to detect a T>C transition determining a PstI polymorphism at the 3'UTR of POU1F1 locus and evaluate its associations with cashmere traits in 847 Inner Mongolia White Cashmere goats. In the analyzed population, the allelic frequencies for the T and C alleles are 0.959 and 0.041, respectively and the genotypic frequencies are in Hardy-Weinberg equilibrium (P > 0.05). Moreover, significant statistical relationships between the PstI polymorphism of POU1F1 gene and goat cashmere yields were found (*P < 0.05). When compared with TC genotype, TT genotype was associated with superior cashmere yields in 2, 4, and 5 years old individuals, as well as with average cashmere yield. Hence, TT genotype is suggested to be a molecular marker for senior cashmere yield.
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, No. 22, Xinong Road, Yangling, Shaanxi, 712100, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Lan
authors.forename: J H
authors.initials: JH
authors.lastname: Shu
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: C Y
authors.initials: CY
authors.lastname: Pan
authors.forename: C Z
authors.initials: CZ
authors.lastname: Lei
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: S Q
authors.initials: SQ
authors.lastname: Liu
authors.forename: Y B
authors.initials: YB
authors.lastname: Zhang
mesh_terms: D020413:3' Untranslated Regions / Q000235:genetics*; D000367:Age Factors; D000818:Animals; D015415:Biomarkers; D015252:Deoxyribonucleases, Type II Site-Specific; D005838:Genotype; D006041:Goats; D006197:Hair / Q000254:growth & development*; D011110:Polymorphism, Genetic; D020641:Polymorphism, Single Nucleotide; D050817:Transcription Factor Pit-1 / Q000235:genetics* / Q000502:physiology
chemical_list: D020413:3' Untranslated Regions; D015415:Biomarkers; D050817:Transcription Factor Pit-1; C024388:CTGCAG-specific type II deoxyribonucleases; D015252:Deoxyribonucleases, Type II Site-Specific