title: Diagnosis of psittacine beak and feather disease (PBFD) viral infection, avian polyomavirus infection, adenovirus infection and herpesvirus infection in psittacine tissues using DNA in situ hybridization.
abstract: The evaluation of the usefulness of DNA probes in a diagnostic setting to identify nuclear inclusions in selected viral infections (psittacine beak and feather disease viral infection, avian polyomavirus infection, adenovirus infection and Pacheco's parrot disease) is reported. A DNA in situ hybridization method was used to detect viral nucleic acid in sections of paraffin-embedded tissues coming from birds naturally and/or experimentally infected. It is concluded that DNA probes used for polyomavirus (FN-19) and adenovirus (FN-23) are able to identify nucleic acid of each virus in the cells with nuclear inclusions, and when used for psittacine beak and feather disease virus (FN-8), and Pacheco's parrot disease virus (FN-49) are able to detect viral nucleic acid in cells with or without inclusions.
authors.affiliation: School of Veterinary Medicine, Universidad Autonoma de Barcelona, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ramis
authors.forename: K S
authors.initials: KS
authors.lastname: Latimer
authors.forename: F D
authors.initials: FD
authors.lastname: Niagro
authors.forename: R P
authors.initials: RP
authors.lastname: Campagnoli
authors.forename: B W
authors.initials: BW
authors.lastname: Ritchie
authors.forename: D
authors.initials: D
authors.lastname: Pesti