[Effect of cetirizine hydrochloride on the expression of substance P receptor and cytokines production in human epidermal keratinocytes and dermal fibroblasts].
To investigate the effect of cetirizine hydrochloride on the expression of neurokinin 1 receptor (NK-1R) and cytokines production induced by substance P (SP) in HaCaT cells (a human epidermal keratinocyte cell line) and dermal fibroblasts. The effect of cetirizine on the expression of NK-1R protein was detected by flow cytometry and Western blotting analysis. The modulation of cetirizine on the production of interferon (IFN)-gamma, interleukin (IL)-1beta, IL-6 and IL-8 in HaCaT cells and fibroblasts was measured by ELISA. The results showed that cetirizine significantly inhibited the expression of NK-1R in HaCaT cells and fibroblasts. SP induced the production of IFN-gamma, IL-1beta and IL-8 in both cell types. Cetirizine 1-100 micromol x L(-1) inhibited SP-induced IL-1beta and IL-8 production in HaCaT cells and fibroblasts, while had no effect on the production of IFN-gamma in both cells. Both SP and cetirizine had no effect on the secretion of IL-6 in HaCaT cells and fibroblasts. These findings suggest that cetirizine may be involved in the treatment of SP-induced skin inflammation by inhibiting the expression of substance P receptor and regulation the production of IL-1beta and IL-8 in epidermal keratinocyte and dermal fibroblasts.
Liu
Ji-Yong
JY
Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai 200433, China.
Zhao
Yong-Zhe
YZ
Peng
Cheng
C
Li
Feng-Qian
FQ
Zhu
Quan-Gang
QG
Hu
Jin-Hong
JH
D018926:Anti-Allergic Agents / Q000494:pharmacology; D002460:Cell Line; D017332:Cetirizine / Q000494:pharmacology*; D005347:Fibroblasts / Q000166:cytology / Q000378:metabolism*; D039563:Histamine H1 Antagonists, Non-Sedating / Q000494:pharmacology; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D053583:Interleukin-1beta / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism*; D018040:Receptors, Neurokinin-1 / Q000378:metabolism*; D013373:Substance P / Q000494:pharmacology
D018926:Anti-Allergic Agents; D039563:Histamine H1 Antagonists, Non-Sedating; D053583:Interleukin-1beta; D016209:Interleukin-8; D018040:Receptors, Neurokinin-1; D013373:Substance P; D007371:Interferon-gamma; D017332:Cetirizine