title: The effect of non-surgical periodontal therapy on peroxidase activity in diabetic patients: a case-control pilot study.
abstract: OBJECTIVE
  To evaluate the effect of periodontal therapy on clinical parameters as well as on total salivary peroxidase (TSP) activity and myeloperoxidase (MPO) activity in the gingival crevicular fluid (GCF) of patients with type 2 diabetes mellitus (DM2) and of systemically healthy individuals.
  
  
  METHODS
  Twenty DM2 subjects with inadequate metabolic control (test group) and 20 systemically healthy individuals (control group), both groups with chronic periodontitis, were enrolled. Periodontal clinical parameters, namely periodontal probing depth (PD), clinical attachment level (CAL), visible plaque index (VPI), bleeding on probing (BOP), gingival bleeding index (GBI) and presence of suppuration (SUP), as well as TSP activity and GCF MPO activity, were assessed before and 3 months after non-surgical periodontal therapy.
  
  
  RESULTS
  At baseline and 3 months post-treatment, the test group presented a higher percentage of sites with VPI and BOP (p<0.01). MPO activity in the GCF presented lower values (p<0.05) for the test group at both baseline and the post-treatment period. The periodontal treatment resulted in a significant improvement of most clinical and enzymatic parameters for both groups (p<0.05).
  
  
  CONCLUSIONS
  In both groups, the periodontal therapy was effective in improving most clinical parameters and in reducing salivary and GCF enzymatic activity. The diabetic individuals presented lower MPO activity in the GCF.
authors.affiliation: Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University, Araraquara, SP, Brazil.
authors.forename: Daniela
authors.initials: D
authors.lastname: Gonçalves
authors.forename: Fernanda Oliveira Bello
authors.initials: FO
authors.lastname: Correa
authors.forename: Najeh Maissar
authors.initials: NM
authors.lastname: Khalil
authors.forename: Olga Maria Mascarenhas
authors.initials: OM
authors.lastname: de Faria Oliveira
authors.forename: Silvana Regina Perez
authors.initials: SR
authors.lastname: Orrico
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D002851:Chromatography, High Pressure Liquid; D055113:Chronic Periodontitis / Q000201:enzymology / Q000628:therapy*; D003774:Dental Plaque Index; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology*; D005260:Female; D005500:Follow-Up Studies; D005883:Gingival Crevicular Fluid / Q000201:enzymology; D005884:Gingival Hemorrhage / Q000201:enzymology / Q000628:therapy; D006442:Glycated Hemoglobin / Q000032:analysis; D006801:Humans; D008297:Male; D008875:Middle Aged; D017622:Periodontal Attachment Loss / Q000201:enzymology / Q000628:therapy; D010512:Periodontal Index; D010514:Periodontal Pocket / Q000201:enzymology / Q000628:therapy; D009195:Peroxidase / Q000032:analysis*; D010865:Pilot Projects; D012463:Saliva / Q000201:enzymology*; D013056:Spectrophotometry, Ultraviolet; D013492:Suppuration
chemical_list: D006442:Glycated Hemoglobin; D009195:Peroxidase