One- and two-dimensional 1H-NMR analysis of lipids extracted from erythrocytes and plasma of humans.
A combination of established lipid extraction procedures and high-resolution 1D and 2D NMR spectroscopic methods has been used to analyze qualitatively and quantitatively the lipid composition of human erythrocytes and plasma. The major lipids present in each structural class were identified and quantified. Considerable information on the composition of unsaturated fatty acid chains were obtained for the intact lipids without resort to hydrolysis.
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Dipartimento di Scienze Chimiche, Universitá di Cagliari, via Ospedale 72, 09100 Cagliari, Italy.
Lai
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Pilia
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Casti
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Zedda
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Gibbons
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