title: Effect of haematological alterations on thalassaemia investigation in HIV-1-infected Thai patients receiving antiretroviral therapy.
abstract: OBJECTIVE
  To evaluate the effect of haematological alterations resulting from antiretroviral therapy (ART) on the diagnosis of thalassaemia carriers in HIV-1-infected Thai patients.
  
  
  METHODS
  Complete blood cell counts, osmotic fragility (OF) test and haemoglobin (Hb)-A(2) values were measured in blood samples of 52 antiretroviral-treated and 14 untreated HIV-1-infected patients. Data were analysed according to thalassaemia type and ART.
  
  
  RESULTS
  Sixteen patients carried at least one of the investigated thalassaemia types and most of them (87.5%) received ART. Their red cell indices [mean corpuscular Hb (MCH), mean corpuscular Hb concentration (MCHC) and red blood cell distribution width (RDW)], OF test and Hb-A(2) values were observed within the critical criteria of each thalassaemia type. Normocytic red cells were observed in alpha-thalassaemia and Hb-E trait. Among HIV-1-infected patients who are non-thalassaemia carriers, higher values of Hb-A(2), MCH, macrocytosis and lower red cell counts were observed in the treated group. Values of RDW, MCHC and OF test for treated and untreated groups were in the normal range. Five treated patients had Hb-A(2) values within the critical criteria of beta-thalassaemia carriers but beta-thalassaemia gene mutations were not observed by polymerase chain reaction analysis.
  
  
  CONCLUSIONS
  ART can alter many haematological figures. Therefore, diagnosis of thalassaemia should be evaluated carefully in combination with those parameters.
authors.affiliation: Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, Thailand. sakornmi001@yahoo.com
authors.forename: S
authors.initials: S
authors.lastname: Pornprasert
authors.forename: P
authors.initials: P
authors.lastname: Leechanachai
authors.forename: V
authors.initials: V
authors.lastname: Klinbuayaem
authors.forename: P
authors.initials: P
authors.lastname: Leenasirimakul
authors.forename: K
authors.initials: K
authors.lastname: Sukunthamala
authors.forename: B
authors.initials: B
authors.lastname: Thunjai
authors.forename: A
authors.initials: A
authors.lastname: Phusua
authors.forename: R
authors.initials: R
authors.lastname: Saetung
authors.forename: T
authors.initials: T
authors.lastname: Sanguansermsri
mesh_terms: D000328:Adult; D044966:Anti-Retroviral Agents / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D004909:Erythrocyte Indices; D005260:Female; D015658:HIV Infections / Q000097:blood* / Q000188:drug therapy*; D015497:HIV-1; D006443:Hemoglobin A2 / Q000032:analysis; D006801:Humans; D019259:Lamivudine / Q000627:therapeutic use*; D008297:Male; D019829:Nevirapine / Q000627:therapeutic use*; D009996:Osmotic Fragility; D018119:Stavudine / Q000627:therapeutic use*; D013789:Thalassemia / Q000097:blood* / Q000175:diagnosis*; D055815:Young Adult
chemical_list: D044966:Anti-Retroviral Agents; D015415:Biomarkers; C516355:stavudine, lamivudine, nevirapine drug combination; D019259:Lamivudine; C049102:hemoglobin A2'; D006443:Hemoglobin A2; D019829:Nevirapine; D018119:Stavudine