title: Enzyme-functionalized mesoporous silica for bioanalytical applications.
abstract: The unique properties of mesoporous silica materials (MPs) have attracted substantial interest for use as enzyme-immobilization matrices. These features include high surface area, chemical, thermal, and mechanical stability, highly uniform pore distribution and tunable pore size, high adsorption capacity, and an ordered porous network for free diffusion of substrates and reaction products. Research demonstrated that enzymes encapsulated or entrapped in MPs retain their biocatalytic activity and are more stable than enzymes in solution. This review discusses recent advances in the study and use of mesoporous silica for enzyme immobilization and application in biosensor technology. Different types of MPs, their morphological and structural characteristics, and strategies used for their functionalization with enzymes are discussed. Finally, prospective and potential benefits of these materials for bioanalytical applications and biosensor technology are also presented.
authors.affiliation: Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USA.
authors.forename: Cristina
authors.initials: C
authors.lastname: Ispas
authors.forename: Igor
authors.initials: I
authors.lastname: Sokolov
authors.forename: Silvana
authors.initials: S
authors.lastname: Andreescu
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004798:Enzymes / Q000378:metabolism*; D010316:Particle Size; D016062:Porosity; D012822:Silicon Dioxide / Q000138:chemical synthesis / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D004798:Enzymes; D012822:Silicon Dioxide