A highly conserved baculovirus gene p48 (ac103) is essential for BV production and ODV envelopment.
Autographa californica multiple nucleopolyhedrovirus (AcMNPV) p48 (ac103) is a highly conserved baculovirus gene of unknown function. In the present study, we generated a knockout of the p48 gene in an AcMNPV bacmid and investigated the role of P48 in baculovirus life cycle. The p48-null Bacmid vAc(P48-KO-PH-GFP) was unable to propagate in cell culture, while a 'repair' Bacmid vAc(P48-REP-PH-GFP) was able to replicate in a manner similar to a wild-type Bacmid vAc(PH-GFP). Titration assays and Western blotting confirmed that vAc(P48-KO-PH-GFP) was unable to produce budded viruses (BVs). qPCR analysis showed that p48 deletion did not affect viral DNA replication. Electron microscopy indicated that P48 was required for nucleocapsid envelopment to form occlusion-derived viruses (ODVs) and their subsequent occlusion. Confocal analysis showed that P48 prominently condensed in the centre of the nucleus. Our results demonstrate that P48 plays an essential role in BV production and ODV envelopment in the AcMNPV life cycle.
Yuan
Meijin
M
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Wu
Wenbi
W
Liu
Chao
C
Wang
Yanjie
Y
Hu
Zhaoyang
Z
Yang
Kai
K
Pang
Yi
Y
D016367:Baculoviridae / Q000235:genetics / Q000502:physiology*; D002467:Cell Nucleus / Q000737:chemistry; D017124:Conserved Sequence; D017353:Gene Deletion; D020043:Genes, Essential; D005814:Genes, Viral; D005816:Genetic Complementation Test; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D016254:Mutagenesis, Insertional; D019251:Nucleocapsid / Q000648:ultrastructure; D014764:Viral Proteins / Q000032:analysis; D014771:Virion / Q000648:ultrastructure; D019065:Virus Assembly; D014779:Virus Replication
D014764:Viral Proteins