title: Colonic perianastomotic carcinogenesis in an experimental model.
abstract: BACKGROUND
  To examine the effect of anastomosis on experimental carcinogenesis in the colon of rats.
  
  
  METHODS
  Forty-three 10-week-old male and female Sprague-Dawley rats were operated on by performing an end-to-side ileorectostomy. Group A:16 rats received no treatment. Group B: 27 rats received 18 subcutaneous injections weekly at a dose of 21 mg/kg wt of 1-2 dimethylhydrazine (DMH), from the eighth day after the intervention. Animals were sacrificed between 25-27 weeks. The number of tumours, their localization, size and microscopic characteristics were recorded. A paired chi-squared analysis was performed comparing tumoral induction in the perianastomotic zone with the rest of colon with faeces.
  
  
  RESULTS
  No tumours appeared in the dimethylhydrazine-free group. The percentage tumoral area was greater in the perianastomotic zone compared to tumours which had developed in the rest of colon with faeces (p = 0.014).
  
  
  CONCLUSIONS
  We found a cocarcinogenic effect due to the creation of an anastomosis, when using an experimental model of colonic carcinogenesis induced by DMH in rats.
authors.affiliation: Department of Surgery, Hospital Valle Del Nalón, Langreo, Asturias, Spain. sergio.perez-holanda@sespa.princast.es
authors.forename: Sergio
authors.initials: S
authors.lastname: Pérez-Holanda
authors.forename: Luis
authors.initials: L
authors.lastname: Rodrigo
authors.forename: Carme
authors.initials: C
authors.lastname: Pinyol-Felis
authors.forename: Joan
authors.initials: J
authors.lastname: Vinyas-Salas
mesh_terms: D019813:1,2-Dimethylhydrazine / Q000494:pharmacology; D000714:Anastomosis, Surgical; D000818:Animals; D002273:Carcinogens; D002277:Carcinoma / Q000139:chemically induced* / Q000473:pathology*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
chemical_list: D002273:Carcinogens; D019813:1,2-Dimethylhydrazine