title: Comparison of 2 moxifloxacin regimens for preoperative prophylaxis: prospective randomized triple-masked trial. Part 2: residual conjunctival flora.
abstract: OBJECTIVE
  To determine whether a regimen of topical moxifloxacin hydrochloride ophthalmic solution 0.5% (Vigamox) administered on the day of cataract surgery reduces conjunctival bacterial flora.
  
  
  METHODS
  Iladevi Cataract & IOL Research Centre, Ahmedabad, India.
  
  
  METHODS
  Seventy-four patients were prospectively randomized to receive Vigamox 4 times 1 day before surgery plus 1 drop 2 hours preoperatively (Group 1) and 74 patients, to receive Vigamox 2 hours before surgery and every 15 minutes in the first hour only (Group 2). Conjunctival swabs from the upper and lower lid margins and lower fornix were taken before and after Vigamox instillation. Aliquots were spread on chocolate blood, anaerobic basal, and nutrient agars. The number of bacterial colony-forming units (CFU) on the plates was counted with a grid technique and expressed as log values. The total number of CFU per eye and mean percentage reduction in CFU after instillation were calculated. Bacterial species were identified using biochemical and biophysical reactions. Wilcoxon signed ranked and Mann-Whitney tests and multiple linear regression were applied.
  
  
  RESULTS
  In Group 1, the median colony count decreased from 1284.00 CFU +/- 643.18 (SD) before Vigamox instillation to 65.00 +/- 72.61 CFU after instillation (93.12% reduction) (P<.001) and in Group 2, from 1634.00 +/- 769.27 CFU to 75.00 +/- 81.23 CFU (93.28% reduction) (P<.001). The percentage reduction after instillation was similar between the 2 groups (P = .09).
  
  
  CONCLUSIONS
  Both Vigamox regimens reduced the number of CFU of conjunctival flora, indicating that instillation beginning on the day of surgery is effective.
authors.affiliation: Iladevi Cataract & IOL Research Centre, Raghudeep Eye Clinic, Memnagar, Ahmedabad, India. icirc@abhayvasavada.com <icirc@abhayvasavada.com>
authors.forename: Abhay R
authors.initials: AR
authors.lastname: Vasavada
authors.forename: Devarshi
authors.initials: D
authors.lastname: Gajjar
authors.forename: Shetal M
authors.initials: SM
authors.lastname: Raj
authors.forename: Vaishali
authors.initials: V
authors.lastname: Vasavada
authors.forename: Viraj
authors.initials: V
authors.lastname: Vasavada
mesh_terms: D000287:Administration, Topical; D000368:Aged; D000369:Aged, 80 and over; D000890:Anti-Infective Agents / Q000627:therapeutic use*; D019072:Antibiotic Prophylaxis; D001372:Aza Compounds / Q000627:therapeutic use*; D001419:Bacteria / Q000187:drug effects / Q000302:isolation & purification; D002387:Cataract Extraction; D015169:Colony Count, Microbial; D003228:Conjunctiva / Q000187:drug effects* / Q000382:microbiology*; D004812:Epidemiologic Methods; D005143:Eyelids / Q000187:drug effects / Q000382:microbiology; D005260:Female; D024841:Fluoroquinolones; D006801:Humans; D008297:Male; D008875:Middle Aged; D000077266:Moxifloxacin; D009883:Ophthalmic Solutions / Q000627:therapeutic use*; D011446:Prospective Studies; D011804:Quinolines / Q000627:therapeutic use*
chemical_list: D000890:Anti-Infective Agents; D001372:Aza Compounds; D024841:Fluoroquinolones; D009883:Ophthalmic Solutions; D011804:Quinolines; D000077266:Moxifloxacin