[mRNA expression level of nucleostemin in esophageal squamous cell carcinoma tissue].
OBJECTIVE
To investigate the mRNA expression levels of nucleostemin (NS) in human esophageal squamous cell carcinoma tissue.


METHODS
Real-time PCR was used to quantify the mRNA expression of NS in the samples of esophageal squamous cell carcinoma tissue and their matched normal esophageal mucosa tissue from 62 patients, 36 males and 26 females, aged (61 +/- 10) (38-75). The relationship between NS mRNA expression level and clinical pathological features was analyzed.


RESULTS
The NS mRNA expression level of the 62 cases of esophageal squamous cell carcinoma tissue was(4.5 +/- 2.1), significantly higher than that of the matched normal esophageal mucosa tissue [(2.1 +/- 1.3), t = -5.045, P = 0.000]. The mRNA expression level of NS was associated with tumor grade, depth of infiltration, and lymph node metastasis (all P < 0.05), but not with gender, age, and pathological type (all P > 0.05). Multiple linear regression analysis revealed that clinical and pathological features influenced the NS mRNA expression level (P = 0. 000), and the depth of infiltration and lymph node metastasis were important influencing factors for NS mRNA expression level(both P < 0.05).


CONCLUSIONS
NS may play an important role in the progression and proliferation of esophageal squamous cell carcinoma.
Zhang
Gong-yuan
GY
Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, China.
Chen
Ping-ping
PP
Li
Sheng-lei
SL
Yin
Lei
L
Gao
Dong-ling
DL
Le
Xiao-ping
XP
Chen
Kui-sheng
KS
Zhang
Yun-han
YH
Zhang
Qin-xian
QX
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000473:pathology*; D002352:Carrier Proteins / Q000235:genetics*; D004938:Esophageal Neoplasms / Q000235:genetics / Q000473:pathology*; D005260:Female; D019204:GTP-Binding Proteins; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D002352:Carrier Proteins; C469949:GNL3 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D019204:GTP-Binding Proteins