Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood.
Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35-40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aquabacterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides-clostridium-lactobacillus cluster, which is indigenous to gastrointestinal flora.
Moriyama
Kosei
K
Nakamura Gakuen University, Faculty of Nutritional Sciences, Fukuoka, Japan.
Ando
Chie
C
Tashiro
Kosuke
K
Kuhara
Satoru
S
Okamura
Seiichi
S
Nakano
Shuji
S
Takagi
Yasumitsu
Y
Miki
Takeyoshi
T
Nakashima
Yoshiyuki
Y
Hirakawa
Hideki
H
D016470:Bacteremia / Q000175:diagnosis*; D001419:Bacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D001769:Blood / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics*
D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S