title: Markers of glycemic control in the mouse: comparisons of 6-h- and overnight-fasted blood glucoses to Hb A1c.
abstract: The present studies examined the relationship between fasting blood glucose and Hb A(1c) in C57BL/6J, DBA/2J, and KK/HlJ mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of 6-h vs. overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with Hb A(1c), as determined with a hand-held automated device using an immunodetection method. R(2) values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J, respectively. Six-hour fasting blood glucose correlated more closely with the level of daily averaged blood glucose and with Hb A(1c) than did blood glucose following an overnight fast. To validate the immunoassay-determined Hb A(1c), we also measured total glycosylated hemoglobin using boronate HPLC. Hb A(1c) values correlated well with total glycosylated hemoglobin in all three strains but were relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice. These results show that 6-h fasting glucose provides a superior index of glycemic control and correlates more closely with Hb A(1c) than overnight-fasted blood glucose in these strains of mice.
authors.affiliation: Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA.
authors.forename: Byoung Geun
authors.initials: BG
authors.lastname: Han
authors.forename: Chuan-Ming
authors.initials: CM
authors.lastname: Hao
authors.forename: Elena E
authors.initials: EE
authors.lastname: Tchekneva
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Wang
authors.forename: Chieh Allen
authors.initials: CA
authors.lastname: Lee
authors.forename: Benyamin
authors.initials: B
authors.lastname: Ebrahim
authors.forename: Raymond C
authors.initials: RC
authors.lastname: Harris
authors.forename: Timothy S
authors.initials: TS
authors.lastname: Kern
authors.forename: David H
authors.initials: DH
authors.lastname: Wasserman
authors.forename: Matthew D
authors.initials: MD
authors.lastname: Breyer
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Qi
mesh_terms: D000818:Animals; D015415:Biomarkers; D001786:Blood Glucose / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000378:metabolism; D005215:Fasting / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011237:Predictive Value of Tests
chemical_list: D015415:Biomarkers; D001786:Blood Glucose; D005947:Glucose; D006442:Glycated Hemoglobin