title: High-resolution NMR "chromatography" using a liquids spectrometer.
abstract: NMR spectroscopy is an excellent tool for the structural analysis of pure compounds. However, for mixtures it performs poorly because of overlapping signals. Diffusion can be used to separate compounds of widely differing molecular weight but the amount of separation is usually insufficient. Addition of a solid medium, analogous to the stationary phase in chromatography, can preferentially slow the diffusion of some components of a mixture permitting separation in the diffusion dimension. However, this would usually require a solid-state NMR spectrometer otherwise the signals would be too broad. Susceptibility matching the solvent to the solid medium yields a spectrum with narrow signals allowing the measurement of a DOSY spectrum with enhanced separation in the diffusion dimension.
authors.affiliation: Department of Organic Chemistry, E Safra Campus, Givat Ram, The Hebrew University of Jerusalem, Jerusalem, Israel. royh63@gmail.com
authors.forename: Roy E
authors.initials: RE
authors.lastname: Hoffman
authors.forename: Hilla
authors.initials: H
authors.lastname: Arzuan
authors.forename: Chava
authors.initials: C
authors.lastname: Pemberton
authors.forename: Abraham
authors.initials: A
authors.lastname: Aserin
authors.forename: Nissim
authors.initials: N
authors.lastname: Garti
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012822:Silicon Dioxide / Q000737:chemistry*; D012996:Solutions; D013048:Specimen Handling / Q000379:methods*
chemical_list: D012996:Solutions; D012822:Silicon Dioxide