title: Anti-bacterial and cytotoxic properties of plasma sprayed silver-containing HA coatings.
abstract: Silver-containing hydroxyapatite (HA) coatings have been prepared on titanium substrate by vacuum plasma spraying (VPS) method and anti-bacterial properties of the coatings were examined. Three types of bacteria stains, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus, were employed in this test. The results showed that the silver-containing HA coatings exhibited significant anti-bacterial effects against the three bacteria with anti-bacterial ratios higher than 95%. The release of silver ions in the physiological environment ensured excellent anti-bacterial properties of the silver-containing HA coatings. International standard ISO 10993-12 was adopted for cytotoxicity evaluation using fibroblast cell line L929, and it was found that the cytotoxicity for the coatings ranked 0 that showed no cytotoxicity for the coatings. Hemolysis test was processed according to ASTM F 756 standard with anti-coagulated rabbit blood, and the hemolysis ratios of the coatings were below 0.4%, indicating of non-hemolysis for the coatings.
authors.affiliation: Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai, 200050, China. chenyikai7@hotmail.com
authors.forename: Yikai
authors.initials: Y
authors.lastname: Chen
authors.forename: Xuebin
authors.initials: X
authors.lastname: Zheng
authors.forename: Youtao
authors.initials: Y
authors.lastname: Xie
authors.forename: Chuanxian
authors.initials: C
authors.lastname: Ding
authors.forename: Hongjiang
authors.initials: H
authors.lastname: Ruan
authors.forename: Cunyi
authors.initials: C
authors.lastname: Fan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D003603:Cytotoxins / Q000494:pharmacology*; D017886:Durapatite / Q000737:chemistry*; D004926:Escherichia coli / Q000187:drug effects; D007739:L Cells; D008422:Materials Testing; D051379:Mice; D010316:Particle Size; D010949:Plasma; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D012834:Silver / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects; D013379:Substrate Specificity; D013499:Surface Properties; D013997:Time Factors; D014025:Titanium / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D003603:Cytotoxins; D012834:Silver; D017886:Durapatite; D014025:Titanium