Determination of log D via automated microfluidic liquid-liquid extraction.
A method for log D (pH 7.4) measurement was developed using microfluidic liquid-liquid extraction. Values were determined for 26 compounds and compared to results obtained via shake-flask methods. Excellent correlation between the values obtained via both methods was achieved (R(2) = 0.994). The developed methodology is amenable to automation, enabling high-throughput determination of large compound collections.
Alimuddin
Muhammad
M
Pfizer Inc., 10770 Science Center Drive, San Diego, California 92121, USA.
Grant
Daniel
D
Bulloch
Daryl
D
Lee
Noelle
N
Peacock
Martin
M
Dahl
Russell
R
D001331:Automation; D001702:Biopharmaceutics / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000379:methods*; D010599:Pharmacokinetics