Creation of a gelatin-based solution for preservation of leukocytes.
A new effective and available method is proposed for preservation of human blood leukocytes at supercooling temperature (-10 degrees C). The method consists in induction of cold hypobiosis under protection of an original antifreeze protecting solution containing glycerol (cryoprotector), mexidol (antioxidant and membrane stabilizer), and gelatin preparation (modegel or gelatinol) as the solvent. No washing of the solution from the biological object before its intravenous injection is needed; the solution preserves morphological integrity of cells and their functional activity at a high level for 12 days.
Svedentsov
E P
EP
Laboratory of Blood Cryophysiology, Institute of Physiology, Komi Research Center, Ural Division of Russian Academy of Sciences, Syktvvkar.
Stepanova
E S
ES
Tumanova
T V
TV
Zaytseva
O O
OO
Solomina
O N
ON
D001793:Blood Preservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D006801:Humans; D007962:Leukocytes / Q000166:cytology* / Q000378:metabolism; D015203:Reproducibility of Results; D012996:Solutions / Q000737:chemistry*; D013997:Time Factors
D003451:Cryoprotective Agents; D012996:Solutions; D005780:Gelatin