Intrinsic ruminal innervation in ruminants of different feeding types.
According to their feeding habits, ruminants can be classified as grazers, concentrate selectors and those of intermediate type. The different feeding types are reflected in distinct anatomical properties of the forestomachs. The present study was designed to investigate whether the intrinsic innervation patterns of the rumen (the main part of the forestomach) differ between intermediate types and grazers. Myenteric plexus preparations from the rumen of goats (intermediate type), fallow deer (intermediate type), cattle (grazer) and sheep (grazer) were analysed by immunohistochemical detection of the following antigens: Hu-protein (HuC/D), choline acetyltransferase (ChAT), nitric oxide synthase (NOS), vasoactive intestinal peptide (VIP), neuropeptide Y (NPY), substance P (SP), calbindin (CALB) and somatostatin (SOM). Myenteric ganglia of cattle contained 73 +/- 6 neurons per ganglion, whereas the ganglia of sheep were significantly smaller (45 +/- 18 neurons per ganglion). The ganglion density of the myenteric plexus was highest in fallow deer (15 +/- 3 ganglia per cm(2)) and lowest in cattle (6 +/- 1 ganglia per cm(2)). All myenteric neurons were either ChAT or NOS positive. The proportion of NOS-positive neurons was significantly lower in sheep (29.5 +/- 8.2% of all neurons) than in goats (44.2 +/- 9.8%). In all species, additional analysis of the different neuropeptides revealed the following subpopulations in descending order of percentile appearance: ChAT/SP > NOS/VIP/NPY > ChAT/- > NOS/NPY. Expression of CALB was detected in a minority of the ChAT-positive neurons in all species. Somatostatin immunoreactive somata were found only in preparations obtained from fallow deer and sheep. These data suggest that the rumen of grazers is under stronger cholinergic control than the rumen of species belonging to the intermediate type, although most subpopulations of neurons are present in all species. However, whether the strong mixing patterns of low quality roughage during digestion are enabled by the prominent excitatory input of the rumen of grazers requires elucidation in further studies.
Münnich
Juliane
J
Institute of Veterinary-Physiology, Leipzig University, An den Tierkliniken 7, 04103 Leipzig, Germany.
Gäbel
Gotthold
G
Pfannkuche
Helga
H
D000818:Animals; D015415:Biomarkers / Q000032:analysis; D049628:Body Size; D064026:Calbindins; D002417:Cattle / Q000502:physiology; D002795:Choline O-Acetyltransferase / Q000032:analysis; D003670:Deer / Q000502:physiology; D005247:Feeding Behavior; D006041:Goats / Q000502:physiology; D007150:Immunohistochemistry; D009197:Myenteric Plexus / Q000033:anatomy & histology*; D009478:Neuropeptide Y / Q000032:analysis; D019001:Nitric Oxide Synthase / Q000032:analysis; D012417:Rumen / Q000294:innervation*; D012418:Ruminants / Q000502:physiology*; D064030:S100 Calcium Binding Protein G / Q000032:analysis; D012756:Sheep / Q000502:physiology; D013004:Somatostatin / Q000032:analysis; D013045:Species Specificity; D013373:Substance P; D014660:Vasoactive Intestinal Peptide / Q000032:analysis
D015415:Biomarkers; D064026:Calbindins; D009478:Neuropeptide Y; D064030:S100 Calcium Binding Protein G; D013373:Substance P; D014660:Vasoactive Intestinal Peptide; D013004:Somatostatin; D019001:Nitric Oxide Synthase; D002795:Choline O-Acetyltransferase