[Study on endoplasmic reticulum localization of CpTI protein in sck transgenic rice].
OBJECTIVE
To study the endoplasmic reticulum (ER) localization of CpTI protein in sck transgenic rice.


METHODS
Immunohistochemistry and immune electron microscopy (IEM) were used to observe the localization of CpTI protein of root and leaf cell in sck transgenic rice.


RESULTS
The endoplasmic reticulum localization of CpTI protein were observed by IEM. Colloidal gold granules were found in ER of root cell of sck transgenic rice and in root cells of the control plant colloidal gold granules were not observed. In plastid, chloroplast, cytoplasm, karyon of root cell of sck transgenic rice, a little of colloidal gold granules were also observed. The ER in the leaf cell was not observed because of the presence of vacuole, but some colloidal gold granules in cytoplasm, karyon and plastid of leaf cells were observed in sck transgenic rice. Colloidal gold granules were not observed in leaf cells of the control plant.


CONCLUSIONS
It seemed that CpTI protein was mainly localized in the ER of the cell from sck transgenic rice and in other organelles there was also a little CpTI protein.
Wang
Rui
R
Office of Disease Control and Emergency Response, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Zhuo
Qin
Q
Piao
Jianhua
J
Yang
Xiaoguang
X
D004721:Endoplasmic Reticulum / Q000378:metabolism* / Q000648:ultrastructure; D018506:Gene Expression Regulation, Plant; D012275:Oryza / Q000737:chemistry* / Q000235:genetics; D017853:Phosphotransferases (Alcohol Group Acceptor); D010940:Plant Proteins / Q000032:analysis*; D030821:Plants, Genetically Modified / Q000737:chemistry* / Q000235:genetics; D014361:Trypsin Inhibitors / Q000235:genetics / Q000378:metabolism*
D010940:Plant Proteins; D014361:Trypsin Inhibitors; D017853:Phosphotransferases (Alcohol Group Acceptor); C063242:hygromycin-B kinase