title: [Construction of gtfB/CAT eukaryotic expression plasmid of Streptococcus mutans in mammalian cells].
abstract: OBJECTIVE
  The study aimed to evaluate the expression of recombinant plasmid pVAX1- gtfB/CAT in mammalian COS-7 cells.
  
  
  METHODS
  The eukaryotic plasmid carrying encoding gene of gtfB/CAT of Streptococcus mutans was constructed and introduced into COS-7 cells by lipofectamine reagent. The transient protein expression was detected by immunochemistry technique in COS-7 cells.
  
  
  RESULTS
  The positive expression of gefB/CAT was detected in plasma of the cells which were transfected with recombinant plasmid pVAX1- gtfB/CAT. The cells which were transfected with pVAX1 were negative for gtfB/CAT expression.
  
  
  CONCLUSIONS
  GtfB/CAT can be translated and expressed in COS-7 cells after transfected with recombinant plasmid pVAX1- gtfB/CAT. The expressed protein is located in the plasma and the protein is able to combine with anti- gtfB/CAT antibody. The expressed protein has the antigenicity and recombinant plasmid pVAX1- gtfB/CAT is a candidate vaccine.Supported by Key Research Project of Bureau of Education of Guizhou Province (Grant No.2004119).
authors.affiliation: The Affiliated Stomatology Hospital, Wenzhou Medical College. Wenzhou 325027, Zhejiang Province, China. quyunpeng618@163.com
authors.forename: Yun-Peng
authors.initials: YP
authors.lastname: Qu
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Liu
authors.forename: De-Qin
authors.initials: DQ
authors.lastname: Yang
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Ma
authors.forename: Dai-Xiong
authors.initials: DX
authors.lastname: Chen
authors.forename: Qiao-Xue
authors.initials: QX
authors.lastname: Chai
mesh_terms: D000818:Animals; D019556:COS Cells; D002522:Chlorocebus aethiops; D005964:Glucosyltransferases; D010957:Plasmids; D013295:Streptococcus mutans / Q000235:genetics*; D014157:Transcription Factors; D014162:Transfection; D019444:Vaccines, DNA
chemical_list: D014157:Transcription Factors; D019444:Vaccines, DNA; D005964:Glucosyltransferases; C113848:glucosyltransferase D