title: [The cytochrome c oxidase activity in mitochondria of cardiomyocytes of isolated cardiac tissue under long-term hypoxic incubation].
abstract: Using method of electron microscopic histochemistry based upon the oxidative polymerization of 3,3'-diaminobenzidine (DAB) to reveal cytochrome c oxidase activity we identified that long hypoxic incubation of isolated small pieces of cardiac tissue during 72 h caused changes in mitochondrial ultrastructure followed by a breach of functional activities of mitochondria, and, in particular, complex IV of the respiratory chain. But for all that, small electron-dense mitochondria appearing inside electron-light mitochondria ("mitochondria inside mitochondria") stained positively for cytochrome c oxidase activity along the full length of cristaes. The results obtained are discussed in connection with conception of changes in the mitochondrial reticulum ultrastructure during mitoptosis.
authors.forename: V B
authors.initials: VB
authors.lastname: Saprunova
authors.forename: I M
authors.initials: IM
authors.lastname: Solodovnikova
authors.forename: L E
authors.initials: LE
authors.lastname: Bakeeva
mesh_terms: D000818:Animals; D015687:Cell Hypoxia; D003576:Electron Transport Complex IV / Q000378:metabolism*; D008854:Microscopy, Electron; D008929:Mitochondria, Heart / Q000378:metabolism* / Q000648:ultrastructure; D032383:Myocytes, Cardiac / Q000502:physiology* / Q000648:ultrastructure; D051381:Rats; D017208:Rats, Wistar
chemical_list: D003576:Electron Transport Complex IV