title: Decreased expression of the IGF-II gene during porcine adipose cell differentiation.
abstract: IGF-I and IGF-II are known to regulate cell development and recent data suggest a possible role of IGF-II on adipose tissue development. This study was undertaken to examine the IGF system gene expression in porcine differentiating adipocytes. Both adipocytes and stromal-vascular (s/v) cells were isolated from subcutaneous adipose tissue collected from 7-day-old piglets. s/v cells were cultured in chemically defined medium. Compared with isolated adipocytes, IGF-II and IGFBP-5 mRNA levels were very high in freshly isolated s/v cells, whereas IGF-I mRNA levels were lower in s/v cells than in adipocytes. Between day 0 and day 6 of culture, IGF-II and IGFBP-5 gene expression decreased whereas expression levels of late markers of adipocyte differentiation were up-regulated. Cell differentiation was also associated with an increase in the expression of IGF-I, insulin and IGF receptor genes. The current findings suggest that IGF-I and IGF-II have different effects on porcine adipose cell development.
authors.affiliation: INRA, UMR 1079, F-35000 Rennes, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Gardan
authors.forename: Jacques
authors.initials: J
authors.lastname: Mourot
authors.forename: Isabelle
authors.initials: I
authors.lastname: Louveau
mesh_terms: D017667:Adipocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics*; D002469:Cell Separation; D002478:Cells, Cultured; D005786:Gene Expression Regulation / Q000187:drug effects; D018969:Insulin-Like Growth Factor Binding Proteins / Q000235:genetics / Q000378:metabolism; D007334:Insulin-Like Growth Factor I / Q000235:genetics / Q000378:metabolism / Q000494:pharmacology; D007335:Insulin-Like Growth Factor II / Q000235:genetics* / Q000378:metabolism / Q000494:pharmacology; D008291:Malate Dehydrogenase / Q000378:metabolism; D008297:Male; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D017451:Receptors, Somatomedin / Q000235:genetics / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000201:enzymology; D013552:Swine / Q000235:genetics*
chemical_list: D015415:Biomarkers; D018969:Insulin-Like Growth Factor Binding Proteins; D012333:RNA, Messenger; D017451:Receptors, Somatomedin; D007334:Insulin-Like Growth Factor I; D007335:Insulin-Like Growth Factor II; D008291:Malate Dehydrogenase