Detection of possible AI-2-mediated quorum sensing system in commensal intestinal bacteria.
The Vibrio harveyi strain BB170-autoinducer bioassay was used to detect possible quorum sensing autoinducer-2 molecule (AI-2) in culture fluids of commensal intestinal bacteria. Culture fluids of Bacteroides vulgatus, Clostridium proteoclasticum, Escherichia coli, Eubacterium rectale, Lachnospira multipara, Pseudobutyrivibrio ruminis, Roseburia intestinalis, Ruminococcus albus and Ruminococcus flavefaciens contained AI-2-like molecules. The PCR bands from some of the tested strains could be also amplified using primers designed for the luxS gene. These findings suggest that AI-2 is present in the gastrointestinal tract; however, it has not yet been proved whether it is used for bacterial cell-to-cell communication.
Lukás
F
F
Institute of Animal Physiology and Genetics, v.v.i., Academy of Science of the Czech Republic, 142 20 Prague, Czechia. lukas@iapg.cas.cz
Gorenc
G
G
Kopecný
J
J
D001421:Bacteria, Anaerobic / Q000235:genetics / Q000254:growth & development* / Q000302:isolation & purification / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D013437:Carbon-Sulfur Lyases / Q000235:genetics / Q000378:metabolism; D003470:Culture Media; D015964:Gene Expression Regulation, Bacterial; D006714:Homoserine / Q000031:analogs & derivatives* / Q000378:metabolism; D007422:Intestines / Q000382:microbiology*; D007783:Lactones / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D053038:Quorum Sensing; D014733:Vibrio / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D003470:Culture Media; D007783:Lactones; C091088:N-octanoylhomoserine lactone; D006714:Homoserine; D013437:Carbon-Sulfur Lyases; C117753:LuxS protein, Bacteria