title: Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting.
abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral 'fingerprints' for twelve Penicillium species. Prior to MALDI-TOF MS analysis, eight replicate cultures of each Penicillium species were subjected to three one-minute bead-beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contained abundant peaks in the range of m/z 5000-20 000, and allowed unambiguous discrimination between species. In addition, a biomarker common to all Penicillium mass spectra was observed at m/z 13 900. Discriminant analysis using the MALDI-TOF MS data yielded classification error rates of 0% (i.e. 100% correct identification), indicating that MALDI-TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance.
authors.affiliation: Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505, USA. jhettick@cdc.gov
authors.forename: Justin M
authors.initials: JM
authors.lastname: Hettick
authors.forename: Brett J
authors.initials: BJ
authors.lastname: Green
authors.forename: Amanda D
authors.initials: AD
authors.lastname: Buskirk
authors.forename: Michael L
authors.initials: ML
authors.lastname: Kashon
authors.forename: James E
authors.initials: JE
authors.lastname: Slaven
authors.forename: Erika
authors.initials: E
authors.lastname: Janotka
authors.forename: Francoise M
authors.initials: FM
authors.lastname: Blachere
authors.forename: Detlef
authors.initials: D
authors.lastname: Schmechel
authors.forename: Donald H
authors.initials: DH
authors.lastname: Beezhold
mesh_terms: D016000:Cluster Analysis; D005656:Fungal Proteins / Q000737:chemistry*; D010407:Penicillium / Q000302:isolation & purification / Q000378:metabolism*; D010449:Peptide Mapping; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
chemical_list: D005656:Fungal Proteins