title: Incorporating gene functions into regression analysis of DNA-protein binding data and gene expression data to construct transcriptional networks.
abstract: Useful information on transcriptional networks has been extracted by regression analyses of gene expression data and DNA-protein binding data. However, a potential limitation of these approaches is their assumption on the common and constant activity level of a transcription factor (TF) on all the genes in any given experimental condition; for example, any TF is assumed to be either an activator or a repressor, but not both, while it is known that some TFs can be dual regulators. Rather than assuming a common linear regression model for all the genes, we propose using separate regression models for various gene groups; the genes can be grouped based on their functions or some clustering results. Furthermore, to take advantage of the hierarchical structure of many existing gene function annotation systems, such as Gene Ontology (GO), we propose a shrinkage method that borrows information from relevant gene groups. Applications to a yeast dataset and simulations lend support for our proposed methods. In particular, we find that the shrinkage method consistently works well under various scenarios. We recommend the use of the shrinkage method as a useful alternative to the existing methods.
authors.affiliation: Division of Biostatistics, School of Public Health, University of Minnesota, A460 Mayo Building, MMC 303, Minneapolis, MN 55455-0378, USA. pengw@biostat.umn.edu
authors.forename: Peng
authors.initials: P
authors.lastname: Wei
authors.forename: Wei
authors.initials: W
authors.lastname: Pan
mesh_terms: D001665:Binding Sites; D003198:Computer Simulation; D004247:DNA / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D020869:Gene Expression Profiling / Q000379:methods*; D008954:Models, Biological; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D011485:Protein Binding; D012044:Regression Analysis; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D014157:Transcription Factors; D004247:DNA