title: Quantitative in vivo islet potency assay in normoglycemic nude mice correlates with primary graft function after clinical transplantation.
abstract: Reliable assays are critically needed to monitor graft potency in islet transplantation (IT). We tested a quantitative in vivo islet potency assay (QIVIPA) based on human C-peptide (hCP) measurements in normoglycemic nude mice after IT under the kidney capsule. QIVIPA was initially tested by transplanting incremental doses of human islets. hCP levels in mice were correlated with the number of transplanted islet equivalents (r(2) = 0.6, P<0.01). We subsequently evaluated QIVIPA in eight islet preparations transplanted in type 1 diabetic patients. Conversely to standard criteria including islet mass, viability, purity, adenosine triphosphate content, or glucose stimulated insulin secretion, hCP in mice receiving 1% of the final islet product was correlated to primary graft function (hCP increase) after IT (r(2)=0.85, P<0.01). QIVIPA appears as a reliable test to monitor islet graft potency, applicable to validate new methods to produce primary islets or other human insulin secreting cells.
authors.affiliation: INSERM U859, Thérapie Cellulaire du Diabète, Faculté de Médecine, Lille, France.
authors.forename: Robert
authors.initials: R
authors.lastname: Caiazzo
authors.forename: Valery
authors.initials: V
authors.lastname: Gmyr
authors.forename: Bertrand
authors.initials: B
authors.lastname: Kremer
authors.forename: Thomas
authors.initials: T
authors.lastname: Hubert
authors.forename: Benoit
authors.initials: B
authors.lastname: Soudan
authors.forename: Bruno
authors.initials: B
authors.lastname: Lukowiak
authors.forename: Brigitte
authors.initials: B
authors.lastname: Vandewalle
authors.forename: Marie-Christine
authors.initials: MC
authors.lastname: Vantyghem
authors.forename: Francois
authors.initials: F
authors.lastname: Pattou
authors.forename: Julie
authors.initials: J
authors.lastname: Kerr-Conte
mesh_terms: D000818:Animals; D002096:C-Peptide / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000628:therapy; D003922:Diabetes Mellitus, Type 1 / Q000378:metabolism; D005786:Gene Expression Regulation; D006085:Graft Survival; D006801:Humans; D007328:Insulin / Q000378:metabolism; D007333:Insulin Resistance; D007515:Islets of Langerhans / Q000166:cytology*; D016381:Islets of Langerhans Transplantation / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D011863:Radioimmunoassay; D013997:Time Factors
chemical_list: D002096:C-Peptide; D007328:Insulin