Antibacterial activity of cyclodextrins against Bacillus strains.
Growth of alkaliphilic Bacillus halodurans C-125 both on agar plates and in liquid culture was inhibited by methyl-beta-cyclodextrin (CD). Furthermore, resting cells of the strain were lysed by contact with methyl-beta-CD higher than 10 mM. alpha-CD also showed lysis activity against Bacillus and related strains. The activity was not observed with Gram-negative and Gram-positive bacteria except for Bacillus strains. Fluorescence staining and scanning electron microscopy of cells revealed that methyl-beta-CD disrupted cell membranes, and consequently, the cells were lysed. This is a novel physiological property of CDs.
Zhang
Hui-Min
HM
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC) 2-15, Natsushima, Yokosuka 237-0061, Japan.
Li
Zhijun
Z
Uematsu
Katsuyuki
K
Kobayashi
Tohru
T
Horikoshi
Koki
K
D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001407:Bacillus / Q000187:drug effects*; D002462:Cell Membrane / Q000000981:diagnostic imaging / Q000502:physiology; D005456:Fluorescent Dyes / Q000378:metabolism; D050296:Microbial Viability; D046529:Microscopy, Electron, Transmission; D013194:Staining and Labeling; D014463:Ultrasonography; D047391:alpha-Cyclodextrins / Q000494:pharmacology*; D047392:beta-Cyclodextrins / Q000494:pharmacology*
D000900:Anti-Bacterial Agents; D005456:Fluorescent Dyes; D047391:alpha-Cyclodextrins; D047392:beta-Cyclodextrins; C108732:methyl-beta-cyclodextrin