The effect of N-ethylmaleimide on permeability transition as induced by carboxyatractyloside, agaric acid, and oleate.
In this work, we studied the effect of N-ethylmaleimide on permeability transition. The findings indicate that the amine inhibited the effects of carboxyatractyloside and agaric acid. It is known that these reagents interact with the adenine nucleotide carrier through the cytosolic side. When oleate, which interacts through the matrix side, was used it was found that the amine amplified the effects of oleate on permeability transition. The results also show that N-ethylmaleimide strengthened the inhibition induced by carboxyatractyloside, agaric acid, and oleate on ADP exchange. Furthermore, it was also found that oleate improved the binding of eosin-5-maleimide on the adenine nucleotide translocase.
García
Noemí
N
Departamento de Bioquímica, Instituto Nacional de Cardiología, Ignacio Chávez, Mexico, D.F., 01480, Mexico.
Pavón
Natalia
N
Chávez
Edmundo
E
D000244:Adenosine Diphosphate / Q000378:metabolism; D001278:Atractyloside / Q000031:analogs & derivatives* / Q000378:metabolism / Q000494:pharmacology; D002118:Calcium / Q000378:metabolism; D019343:Citric Acid / Q000031:analogs & derivatives* / Q000494:pharmacology; D004801:Eosine Yellowish-(YS) / Q000031:analogs & derivatives / Q000378:metabolism; D005033:Ethylmaleimide / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism*; D000226:Mitochondrial ADP, ATP Translocases / Q000819:agonists / Q000378:metabolism; D008933:Mitochondrial Swelling / Q000187:drug effects*; D019301:Oleic Acid / Q000494:pharmacology*; D010539:Permeability / Q000187:drug effects*
D001278:Atractyloside; D019343:Citric Acid; D019301:Oleic Acid; C011396:agaric acid; D000244:Adenosine Diphosphate; C033531:eosin maleimide; D000226:Mitochondrial ADP, ATP Translocases; D005033:Ethylmaleimide; C003853:carboxyatractyloside; D002118:Calcium; D004801:Eosine Yellowish-(YS)