title: Absence of H186R polymorphism in exon 4 of the APOBEC3G gene among North Indian individuals.
abstract: AIDS restriction genes have been defined in which allelic variations have been shown to influence infection or disease progression. Members of the APOBEC family of cellular polynucleotide cytidine deaminases (e.g., APOBEC3G) have been identified as a host factor that inhibits HIV-1 replication. It deaminates cytidine to uridine in nascent minus-strand viral DNA, inducing G-to-A hypermutation in the plus-strand viral DNA. The impact of codon-changing variant APOBEC3G H186R polymorphism on HIV-1 susceptibility and progression is not clear. We conducted genetic risk association study in HIV-1-exposed seronegative (HES; n = 50) individuals, HIV-1 seronegative (HSN; n = 320) healthy control, and HIV-1 seropositive patients (HSP; n = 190). The APOBEC3G H186R genotypes were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in DNA extracted from peripheral blood and confirmed by direct sequencing the randomly selected 58 samples. Frequency of rare homozygous RR (mutant type) and HR (heterozygous mutant) genotype was 0% while HH (wild type) was 100% among North Indians. In conclusion, we demonstrated that no genetic H186R polymorphism in exon 4 of APOBEC3G gene is found and therefore neither associated with differential susceptibility to HIV-1 infection/progression among North Indians.
authors.affiliation: Department of Microbiology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, India.
authors.forename: Anurag
authors.initials: A
authors.lastname: Rathore
authors.forename: Animesh
authors.initials: A
authors.lastname: Chatterjee
authors.forename: Naohiko
authors.initials: N
authors.lastname: Yamamoto
authors.forename: Tapan N
authors.initials: TN
authors.lastname: Dhole
mesh_terms: D000071480:APOBEC-3G Deaminase; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D015331:Cohort Studies; D003564:Cytidine Deaminase / Q000235:genetics*; D005091:Exons; D005260:Female; D005838:Genotype; D005843:Geography; D015658:HIV Infections / Q000235:genetics / Q000276:immunology; D018023:HIV Seronegativity; D006679:HIV Seropositivity; D015497:HIV-1 / Q000235:genetics / Q000276:immunology; D006801:Humans; D007194:India; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D017422:Sequence Analysis, DNA
chemical_list: D000071480:APOBEC-3G Deaminase; C464924:APOBEC3G protein, human; D003564:Cytidine Deaminase