Assessment of Aspergillus-specific T cells for diagnosis of invasive aspergillosis in a leukemic child with liver lesions mimicking hepatosplenic candidiasis.
A child with acute myeloid leukemia presented with multiple liver lesions mimicking hepatosplenic candidiasis during the neutropenic phase following the induction chemotherapy. All the available diagnostic tools showed repeatedly negative results, including galactomannan. An enzyme-linked immunospot (ELISPOT) assay showed a high number of Aspergillus-specific T cells producing interleukin-10 [TH2(IL-10)] and a low number of Aspergillus-specific T cells producing gamma interferon [TH1(IFN-gamma)], revealing invasive aspergillosis (IA) before the confirmatory biopsy. A progressive skewing from the predominance of TH2(IL-10) to a predominance of TH1(IFN-gamma) was observed close to the complete resolution of the infection and foreshadowed the outcome. The ELISPOT assay holds promise for diagnosing pediatric IA.
Potenza
Leonardo
L
Department of Oncology and Hematology, University of Modena and Reggio Emilia, Azienda Ospedaliera Policlinico, 41100 Modena, Italy.
Barozzi
Patrizia
P
Rossi
Giulio
G
Palazzi
Giovanni
G
Vallerini
Daniela
D
Riva
Giovanni
G
Cellini
Monica
M
Morselli
Monica
M
Volzone
Francesco
F
Venturelli
Claudia
C
Quadrelli
Chiara
C
Di Pancrazio
Luciana
L
Cano
Maria Carmen
MC
Paolucci
Paolo
P
Torelli
Giuseppe
G
Luppi
Mario
M
D001228:Aspergillosis / Q000175:diagnosis* / Q000276:immunology; D001230:Aspergillus / Q000276:immunology*; D002177:Candidiasis / Q000175:diagnosis; D002648:Child; D003937:Diagnosis, Differential; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism; D016753:Interleukin-10 / Q000378:metabolism; D015470:Leukemia, Myeloid, Acute / Q000150:complications*; D008099:Liver / Q000473:pathology; D013601:T-Lymphocytes / Q000276:immunology*
D016753:Interleukin-10; D007371:Interferon-gamma