The study of oxidization fluorescence sensor with molecular imprinting polymer and its application for 6-mercaptopurine (6-MP) determination.
This paper developed optical fiber sensor based on molecular imprinted polymer as artificial recognition element for the determination of 6-mercaptopurine (6-MP) in human serum. This approach displayed high sensitivity by oxidizing 6-MP to a strong fluorescent compound with H(2)O(2) in the alkaline media. It offered a relatively nice selectivity for 6-MP detection by molecular imprinted polymer's recognition. The relative standard deviation (R.S.D.) was 5% for a same sensor (n=5) when 6-MP concentration was 1.0 x 10(-7)gm L(-1) in serum. The developed method was satisfactorily applied to the determination of 6-MP in human serum without any necessity for sample treatment or time-consuming extraction steps prior to the analysis.
Wang
Li
L
Department of Chemistry, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, PR China.
Zhang
Zhujun
Z
D015374:Biosensing Techniques; D001798:Blood Proteins / Q000032:analysis; D004867:Equipment Design; D005336:Fiber Optic Technology; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000494:pharmacology; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000494:pharmacology; D015122:Mercaptopurine / Q000032:analysis* / Q000737:chemistry; D008863:Microspheres; D055100:Optical Fibers; D010100:Oxygen / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D013050:Spectrometry, Fluorescence / Q000379:methods; D013696:Temperature
D001798:Blood Proteins; D005456:Fluorescent Dyes; D011108:Polymers; D006861:Hydrogen Peroxide; D015122:Mercaptopurine; D010100:Oxygen