Neuroprotective effect of dipeptide AVP(4-5)-NH2 is associated with nerve growth factor and heat shock protein HSP70.
In vitro experiments demonstrated the neuroprotective effect of dipeptide pGlu-Asn-NH2, which corresponded to the N-terminal fragment of the major vasopressin metabolite AVP(4-9). The dipeptide in concentrations of 10(-5)-10(-7) M prevented death of HT-22 immortalized hippocampal neurons under conditions of oxidative stress and protected PC-12 rat pheochromocytoma cells from neurotoxic compound 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine. pGlu-Asn-NH2 in a concentration of 10(-6) M increased the content of endogenous neuroprotective substances, neurotrophin NGF and heat shock protein HSP70 in HT-22 cells. Our results indicate that this dipeptide can be used for the therapy of Parkinson's disease.
Zenina
T A
TA
V. V. Zakusov Institute of Pharmacology, Russian Academy of Medical Sciences, Moscow. zenina_tatyana@mail.ru
Gudasheva
T A
TA
Bukreyev
Ya S
YS
Seredenin
S B
SB
D000818:Animals; D002460:Cell Line; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006624:Hippocampus / Q000166:cytology; D051379:Mice; D020932:Nerve Growth Factor / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism; D016716:PC12 Cells; D010446:Peptide Fragments / Q000494:pharmacology*; D051381:Rats; D014667:Vasopressins / Q000737:chemistry / Q000494:pharmacology
D018840:HSP70 Heat-Shock Proteins; D010446:Peptide Fragments; D014667:Vasopressins; D020932:Nerve Growth Factor