Supercoiling and denaturation of DNA loops.
We study the statistical mechanics of small DNA loops emphasizing the competition between elasticity, supercoiling, and denaturation. Motivated by recent experiments and atomistic molecular dynamics simulation, we propose a new coarse-grained phenomenological model of DNA. We extend the classical elastic rod models to include the possibility of denaturation and nonlinear twist elasticity. Using this coarse-grained model, we obtain a phase diagram in terms of fractional overtwist and loop size that can be used to rationalize a number of experimental results which have also been confirmed by atomistic simulations.
Liverpool
T B
TB
Department of Mathematics, University of Bristol, University Walk, Bristol, UK. t.liverpool@bristol.ac.uk
Harris
S A
SA
Laughton
C A
CA
D016475:3T3 Cells; D000818:Animals; D001696:Biomechanical Phenomena; D048429:Cell Size; D004278:DNA, Superhelical / Q000737:chemistry*; D004548:Elasticity; D005347:Fibroblasts / Q000166:cytology*; D051379:Mice; D008954:Models, Biological; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D012212:Rheology; D014783:Viscosity
D004278:DNA, Superhelical