Integrating the QCM detection with magnetic separation for on-line analysis.
We investigate the feasibility of coupling the quartz crystal microbalance (QCM) with magnetic separation for on-line analysis. A flow cell was integrated with QCM and magnetic force for the analysis of magnetic and nonmagnetic samples. The resonant frequency change ((Delta)f) of QCM was related to the amount of deposited magnetic nanoparticles. This experiment demonstrates that QCM can be used as an on-line detector for magnetic separation. The QCM also gives a characteristic response of the binding between the streptavidin and biotin labeled on the magnetic nanoparticles. Biotin-labeled magnetic nanoparticles were flowed through a gold electrode of QCM to deposit as a matrix for selective capturing streptavidin. The resonant frequency change of QCM was proportional to the amounts of streptavidin captured by biotin. This technique can provide a simple, economic, and automatic method for on-line detection of biomarkers.
Tsai
Hweiyan
H
Department of Applied Chemistry, Chung Shan Medical University, Taichung 402, Taiwan. annetsai@csmu.edu.tw
Lin
Yi
Y
Chang
Hung W
HW
Fuh
C Bor
CB
D015374:Biosensing Techniques / Q000379:methods; D001710:Biotin / Q000737:chemistry; D018189:Immunomagnetic Separation / Q000379:methods*; D053758:Nanoparticles; D011791:Quartz / Q000737:chemistry*; D019809:Streptavidin / Q000737:chemistry
D011791:Quartz; D001710:Biotin; D019809:Streptavidin