Detection of group B streptococci in Lim broth by use of group B streptococcus peptide nucleic acid fluorescent in situ hybridization and selective and nonselective agars.
The sensitivity, specificity, and positive and negative predictive values for the detection of group B streptococci from Lim enrichment broth with sheep blood agar (SBA), with selective Streptococcus agar (SSA), and by a peptide nucleic acid fluorescent in situ hybridization (PNA FISH) assay were as follows: for culture on SBA, 68.4%, 100%, 100%, and 87.9%, respectively; for culture on SSA, 85.5%, 100%, 100%, and 94.1%, respectively; and for the PNA FISH assay, 97.4%, 98.3%, 96.1%, and 98.9%, respectively.
Montague
Naomi S
NS
Department of Pathology, Jackson Memorial Hospital, and the University of Miami Miller School of Medicine, Miami, Florida, USA.
Cleary
Timothy J
TJ
Martinez
Octavio V
OV
Procop
Gary W
GW
D001431:Bacteriological Techniques / Q000379:methods*; D003470:Culture Media / Q000737:chemistry*; D005260:Female; D006801:Humans; D017404:In Situ Hybridization, Fluorescence / Q000379:methods*; D020135:Peptide Nucleic Acids; D011237:Predictive Value of Tests; D011247:Pregnancy; D037841:Pregnant People; D012680:Sensitivity and Specificity; D013290:Streptococcal Infections / Q000175:diagnosis; D013292:Streptococcus agalactiae / Q000235:genetics / Q000302:isolation & purification*
D003470:Culture Media; D020135:Peptide Nucleic Acids