title: Fgf signaling in the zebrafish adult brain: association of Fgf activity with ventricular zones but not cell proliferation.
abstract: The zebrafish adult brain contains numerous neural progenitors and is a good model to approach the general mechanisms of adult neural stem cell maintenance and neurogenesis. Here we use this model to test for a correlation between Fgf signaling and cell proliferation in adult progenitor zones. We report expression of Fgf signals (fgf3,4,8a,8b,17b), receptors (fgfr1-4), and targets (erm, pea3, dusp6, spry1,2,4, and P-ERK) and document that genes of the embryonic fgf8 synexpression group acquire strikingly divergent patterns in the adult brain. We further document the specific expression of fgf3, fgfr1-3, dusp6, and P-ERK in ventricular zones, which contain neural progenitors. In these locations, however, a comparison at the single-cell level of fgfr/P-ERK expression with bromo-deoxy-uridine (BrdU) incorporation and the proliferation marker MCM5 indicates that Fgf signaling is not specifically associated with proliferating progenitors. Rather, it correlates with the ventricular radial glia state, some of which only are progenitors. Together these results stress the importance of Fgf signaling in the adult brain and establish the basis to study its function in zebrafish, in particular in relation to adult neurogenesis.
authors.affiliation: Helmholtz Zentrum muenchen, German Research Center for Environmental Health, Department Zebrafish Neurogenetics, Institute of Developmental Genetics, D-85764 Neuherberg, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Topp
authors.forename: Christian
authors.initials: C
authors.lastname: Stigloher
authors.forename: Anna Z
authors.initials: AZ
authors.lastname: Komisarczuk
authors.forename: Birgit
authors.initials: B
authors.lastname: Adolf
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Becker
authors.forename: Laure
authors.initials: L
authors.lastname: Bally-Cuif
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001921:Brain / Q000502:physiology*; D002455:Cell Division; D002552:Cerebral Ventricles / Q000166:cytology* / Q000502:physiology*; D005346:Fibroblast Growth Factors / Q000235:genetics* / Q000502:physiology; D017930:Genes, Reporter; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics; D049452:Green Fluorescent Proteins / Q000235:genetics; D007031:Hypothalamus / Q000254:growth & development / Q000502:physiology; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008636:Mesencephalon / Q000502:physiology; D017468:Receptors, Fibroblast Growth Factor / Q000235:genetics; D012249:Rhombencephalon / Q000502:physiology; D015398:Signal Transduction; D015027:Zebrafish
chemical_list: D005904:Glial Fibrillary Acidic Protein; D017468:Receptors, Fibroblast Growth Factor; D049452:Green Fluorescent Proteins; D005346:Fibroblast Growth Factors