[Selection of bax siRNA sequences and its influence on aluminum induced glioma cell apoptosis].
OBJECTIVE
To decrease bax expression by RNA interference, and inhibite the apoptosis in glioma cells induced by aluminum.


METHODS
We had designed three siRNA sequences, and transfected them into glioma cells. The optimal sequence was selected based on the results of cell viability at various transfection concentrations and different transfection times. Fluorescent staining was used to detect the transfection efficiency, interference efficiency was determined by QRT-PCR method, and protein expression was measured with immunohistochemistry.


RESULTS
The optimal siRNA sequence was selected based on the cell viability. The transfection efficiency was above 90%, the interference efficiency of bax gene was 62.3%, and the optimal transfection time was 72h after transfection, the optimal transfection concentration was 20nmol/L, besides, there was a significant decrease in Bax protein content after transfection.


CONCLUSIONS
The optimal bax siRNA sequence can effectively inhibit the expression of bax gene and Bax protein, which may decrease apoptosis in aluminum-induced toxicity.
Zhang
Qinli
Q
Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
Shi
Yingtao
Y
Niu
Qiao
Q
D000535:Aluminum / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D005910:Glioma / Q000473:pathology*; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics*; D051028:bcl-2-Associated X Protein / Q000235:genetics* / Q000378:metabolism
C494826:BAX protein, human; D034741:RNA, Small Interfering; D051028:bcl-2-Associated X Protein; D000535:Aluminum