BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways.
Ectopic expression of the Brassica napus BABY BOOM (BBM) AP2/ERF transcription factor is sufficient to induce spontaneous cell proliferation leading primarily to somatic embryogenesis, but also to organogenesis and callus formation. We used DNA microarray analysis in combination with a post-translationally regulated BBM:GR protein and cycloheximide to identify target genes that are directly activated by BBM expression in Arabidopsis seedlings. We show that BBM activated the expression of a largely uncharacterized set of genes encoding proteins with potential roles in transcription, cellular signaling, cell wall biosynthesis and targeted protein turnover. A number of the target genes have been shown to be expressed in meristems or to be involved in cell wall modifications associated with dividing/growing cells. One of the BBM target genes encodes an ADF/cofilin protein, ACTIN DEPOLYMERIZING FACTOR9 (ADF9). The consequences of BBM:GR activation on the actin cytoskeleton were followed using the GFP:FIMBRIN ACTIN BINDING DOMAIN2 (GFP:FABD) actin marker. Dexamethasone-mediated BBM:GR activation induced dramatic changes in actin organization resulting in the formation of dense actin networks with high turnover rates, a phenotype that is consistent with cells that are rapidly undergoing cytoplasmic reorganization. Together the data suggest that the BBM transcription factor activates a complex network of developmental pathways associated with cell proliferation and growth.
Passarinho
Paul
P
Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands.
Ketelaar
Tijs
T
Xing
Meiqing
M
van Arkel
Jeroen
J
Maliepaard
Chris
C
Hendriks
Mieke Weemen
MW
Joosen
Ronny
R
Lammers
Michiel
M
Herdies
Lydia
L
den Boer
Bart
B
van der Geest
Lonneke
L
Boutilier
Kim
K
D000199:Actins / Q000378:metabolism; D017360:Arabidopsis / Q000166:cytology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D029688:Brassica napus / Q000235:genetics*; D049108:Cell Enlargement; D049109:Cell Proliferation; D003599:Cytoskeleton / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006360:Heat-Shock Proteins / Q000235:genetics / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D036226:Seedlings / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
D000199:Actins; D006360:Heat-Shock Proteins; D014157:Transcription Factors