Immunolabeling of imaginal discs.
Drosophila melanogaster imaginal discs are a widely used model system to study signal transduction, developmental, and cell biological processes. An important tool to study these processes is immunolabeling. Fluorochrome-conjugated antibody staining allows the researcher to determine the expression pattern and subcellular localization of several proteins in one imaginal disc. In this chapter, I will describe how to dissect, fix, and immunolabel imaginal discs. The protocol is streamlined so that the whole procedure can be performed in 1 d, including image acquisition.
Klein
Thomas
T
Institute for Genetics, University of Cologne, Cologne, Germany.
D000818:Animals; D019521:Body Patterning; D015509:Developmental Biology / Q000379:methods*; D029721:Drosophila Proteins / Q000378:metabolism; D004331:Drosophila melanogaster / Q000502:physiology*; D004625:Embryo, Nonmammalian / Q000502:physiology*; D018507:Gene Expression Regulation, Developmental; D017344:Genes, Insect; D019476:Insect Proteins / Q000378:metabolism; D007814:Larva / Q000378:metabolism; D008853:Microscopy / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods; D008953:Models, Anatomic; D009024:Morphogenesis; D015398:Signal Transduction
D029721:Drosophila Proteins; D019476:Insect Proteins