Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with HbD: a study of 129 samples.
OBJECTIVE
To compare ion exchange and boronate affinity chromatography for HbA(1c) estimation in patients with type I and II diabetes having hemoglobin D.


METHODS
Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA(1c) estimation in patients with homozygous and heterozygous D disease.


RESULTS
Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA(1c) estimation.


CONCLUSIONS
The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.
Moiz
Bushra
B
Department of Pathology and Microbiology, The Aga Khan University, Karachi, Pakistan. bushra.moiz@aku.edu
Hashmi
Mashhooda Rasool
MR
Sadaf
Shahmina
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D001786:Blood Glucose; D002846:Chromatography, Affinity / Q000379:methods* / Q000592:standards*; D002851:Chromatography, High Pressure Liquid; D002852:Chromatography, Ion Exchange / Q000379:methods* / Q000592:standards*; D003920:Diabetes Mellitus / Q000097:blood*; D005215:Fasting / Q000097:blood; D005260:Female; D006442:Glycated Hemoglobin / Q000032:analysis*; D006455:Hemoglobins, Abnormal / Q000032:analysis*; D006801:Humans; D008297:Male; D008875:Middle Aged
D001786:Blood Glucose; D006442:Glycated Hemoglobin; D006455:Hemoglobins, Abnormal; C032001:hemoglobin D