Mutational analysis of the -10 region from the Mycobacterium tuberculosis lipF promoter.
The promoter driving expression of the Mycobacterium tuberculosis gene lipF (Rv3487c) had previously been identified as being upregulated by acidic stress. Subsequently a 59 base pair (bp) acid inducible minimal promoter region was isolated in which a putative -10 region was identified. In this study we use mutational analysis to investigate the -10 region of the lipF promoter. Mutations within this region lead to a dramatic decrease of promoter activity, while a mutation outside of this region does not affect promoter activity.
Gonzales
Michelle
M
Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
Saviola
Beatrice
B
D004252:DNA Mutational Analysis / Q000379:methods*; D004950:Esterases / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics; D011401:Promoter Regions, Genetic
D004950:Esterases