title: [Oligomeric organization of recombinant human neurotrophins expressed in Escherichia coli cells].
abstract: Genes of human neurotrophins NGF, BDNF, NT-3 were cloned, and the corresponding proteins and their fragments were expressed in Escherichia coli BL-21 (DE3lambda) cells. Their intracellular localization was determined. The conditions for isolation and purification of the target recombinant proteins and for folding of BDNF and NT-3 precursors were selected. The recombinant proprecursors of human neurotrophines have been shown to possess complex oligomeric structure.
authors.forename: D R
authors.initials: DR
authors.lastname: Safina
authors.forename: L M
authors.initials: LM
authors.lastname: Rafieva
authors.forename: A V
authors.initials: AV
authors.lastname: Koval'
authors.forename: E E
authors.initials: EE
authors.lastname: Shkurina
authors.forename: V G
authors.initials: VG
authors.lastname: Dmitrieva
authors.forename: N M
authors.initials: NM
authors.lastname: Raevskaia
authors.forename: E V
authors.initials: EV
authors.lastname: Gasanov
authors.forename: I V
authors.initials: IV
authors.lastname: Demidiuk
authors.forename: S V
authors.initials: SV
authors.lastname: Kostrov
mesh_terms: D001704:Biopolymers; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020932:Nerve Growth Factor / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D020933:Neurotrophin 3 / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D011489:Protein Denaturation; D017510:Protein Folding; D011498:Protein Precursors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
chemical_list: D001704:Biopolymers; D019208:Brain-Derived Neurotrophic Factor; D020933:Neurotrophin 3; D010446:Peptide Fragments; D011498:Protein Precursors; D011994:Recombinant Proteins; D020932:Nerve Growth Factor