An amino acid substitution at position 740 in sigma70 of Ralstonia solanacearum strain OE1-1 affects its in planta growth.
Growth of Ralstonia solanacearum strain OE1-1 in roots after invasion is required for virulence. An Arg740Cys substitution in sigma(70) of OE1-1 resulted in loss of in planta growth and virulence. The negative dominance of mutant sigma(70) over the wild-type protein suggested that the amino acid substitution may affect the in planta growth of OE1-1, leading to a lack of virulence.
Kanda
Ayami
A
Faculty of Agriculture, Kochi University, 200 Monobe, Nankoku, Kochi 783-8502, Japan. yhikichi@cc.kochi-u.ac.jp
Tsuneishi
Kazuhiro
K
Mori
Ai
A
Ohnishi
Kouhei
K
Kiba
Akinori
A
Hikichi
Yasufumi
Y
D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D004251:DNA Transposable Elements; D012321:DNA-Directed RNA Polymerases / Q000235:genetics*; D004926:Escherichia coli / Q000201:enzymology / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity; D005798:Genes, Bacterial; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D010935:Plant Diseases / Q000382:microbiology*; D018517:Plant Roots / Q000382:microbiology; D010957:Plasmids; D012329:RNA, Bacterial / Q000235:genetics; D043368:Ralstonia solanacearum / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development / Q000472:pathogenicity*; D012808:Sigma Factor / Q000235:genetics*; D014026:Nicotiana / Q000382:microbiology; D014774:Virulence / Q000235:genetics
D004251:DNA Transposable Elements; D012329:RNA, Bacterial; D012808:Sigma Factor; C081775:RNA polymerase sigma 70; D012321:DNA-Directed RNA Polymerases