Hard tissue formation of STRO-1-selected rat dental pulp stem cells in vivo.
The objective of this study was to examine hard tissue formation of STRO-1-selected rat dental pulp-derived stem cells, seeded into a calcium phosphate ceramic scaffold, and implanted subcutaneously in mice. Previously, STRO-1 selection was used to obtain a mesenchymal stem cell progenitor subpopulation from primary dental pulp-derived stem cells. In the current study, these cells were cultured with three different media: "BMP-plus" medium containing dexamethasone and 100 ng/mL of rhBMP-2, "odontogenic" medium containing dexamethasone, and "control" medium without supplements. The cell-scaffold complexes were cultured in these media for 1, 4, or 8 days before implantation. Histological analysis demonstrated that the cultures with BMP-plus and 4 days of culture gave the highest percentage of hard tissue formation per implant (36 +/- 9% of pore area). Real-time PCR confirmed these results. In conclusion, STRO-1-selected dental pulp stem cells show effective hard tissue formation in vivo, and a short in vitro culture period and addition of BMP-2 can enhance this effect.
Yang
Xuechao
X
Department of Periodontology and Biomaterials, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
Walboomers
X Frank
XF
van den Beucken
Jeroen J J P
JJ
Bian
Zhuan
Z
Fan
Mingwen
M
Jansen
John A
JA
D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D000954:Antigens, Surface / Q000378:metabolism*; D049109:Cell Proliferation; D003782:Dental Pulp / Q000166:cytology*; D005786:Gene Expression Regulation; D020007:Implants, Experimental; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology / Q000201:enzymology / Q000378:metabolism* / Q000648:ultrastructure; D023822:Tissue Engineering
D000954:Antigens, Surface; D012333:RNA, Messenger; C527498:STRO-1 antigen, rat; D000469:Alkaline Phosphatase