title: Helicobacter pylori genotypes in Lithuanian patients with chronic gastritis and duodenal ulcer.
abstract: OBJECTIVE
  Clinical outcome of Helicobacter pylori (H. pylori) infection might be associated with specific virulence-associated bacterial genotypes. The distribution of different bacterial genotypes varies geographically. The aim of this study was to assess the relationship between cagPAI, vacA, and iceA status and severity of the disease in patients from Lithuania, infected by H. pylori.
  
  
  METHODS
  H. pylori from 81 patients (37 with duodenal ulcer and 44 with chronic gastritis) was isolated from gastric biopsy specimens and cultured. Bacterial genotypes cagPAI, vacA (s and m subtypes) and iceA were analyzed by polymerase chain reaction using specific primers.
  
  
  RESULTS
  The cagPAI was identified in 59.3% of Lithuanian H. pylori strains investigated. H. pylori strains cultured from duodenal ulcer (DU) patients more frequently (P<0.01) contained cagPAI and vacA s1 genotypes (75.7% and 75.7%, respectively) in comparison to isolates from chronic gastritis (CG) patients (45.5% and 40.9%, respectively). Evaluation of nucleotide sequence of the vacA middle-region revealed that vacA s2/m2 genotype was more frequent in CG than in DU patients (56.8% and 24.3%, respectively; P<0.05). We have not found any differences in the frequency of iceA1 genotype between the DU and CG patients (46.0% and 40.9%, respectively; P>0.05).
  
  
  CONCLUSIONS
  Our study suggests that cagPAI and vacA s1 genotypes are associated with peptic ulceration in Lithuanian patients infected by H. pylori.
authors.affiliation: Department of Gastroenterology, Kaunas University of Medicine, Lithuania.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Miciuleviciene
authors.forename: Henrikas
authors.initials: H
authors.lastname: Calkauskas
authors.forename: Laimas
authors.initials: L
authors.lastname: Jonaitis
authors.forename: Gediminas
authors.initials: G
authors.lastname: Kiudelis
authors.forename: Vytas
authors.initials: V
authors.lastname: Tamosiūnas
authors.forename: Antanas
authors.initials: A
authors.lastname: Praskevicius
authors.forename: Limas
authors.initials: L
authors.lastname: Kupcinskas
authors.forename: Duglas
authors.initials: D
authors.lastname: Berg
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001483:Base Sequence; D016009:Chi-Square Distribution; D002908:Chronic Disease; D003627:Data Interpretation, Statistical; D004381:Duodenal Ulcer / Q000382:microbiology*; D005260:Female; D005756:Gastritis / Q000382:microbiology*; D005838:Genotype; D016480:Helicobacter pylori / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D006801:Humans; D008097:Lithuania; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D021382:Protein Sorting Signals; D013270:Stomach / Q000382:microbiology; D014774:Virulence
chemical_list: D000942:Antigens, Bacterial; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D021382:Protein Sorting Signals; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori; C063113:ice nucleation protein