Phylogeny of gamma-polyglutamic acid-producing Bacillus strains isolated from a fermented locust bean product manufactured in West Africa.
Twenty-five Bacillus strains capable of producing gamma-polyglutamic acid (PGA) were isolated from fermented locust bean products manufactured in the savanna area of Ghana. To clarify the phylogeny of these PGA-producing strains, phylogenetic analyses based on sequences of 16S rDNA, rpoB (RNA polymerase beta-subunit) and fus (elongation factor G) genes were performed. A phylogenetic tree based on 16S rDNA indicated that ten isolates were clustered in the same group of Bacillus subtilis. Another ten isolates were located in the cluster of B. amyloliquefaciens, and the remaining isolates were identified as B. pumilus (three isolates) and B. licheniformis (two isolates), respectively. Phylogenetic trees based on the partial sequences of rpoB and fus genes were similar to the phylogeny based on 16S rDNA sequences. Thirty-four strains in 27 species belonging to the genus Bacillus and its neighbors were also investigated for PGA production. It was found that PGA was produced by B. amyloliquefaciens NBRC 14141 and NBRC 15535(T), B. atrophaeus NBRC 15539(T), B. licheniformis NBRC 12107, B. mojavensis NBRC 15718(T), B. pumilus NBRC 12094, B. subtilis NBRC 16449, and Lysinibacillus sphaericus NBRC 3525. Except for L. sphaericus, the above Bacillus species are very closely related in phylogeny, indicating that PGA-producing Bacillus strains constitute a cluster.
Meerak
Jomkhwan
J
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Japan.
Yukphan
Pattaraporn
P
Miyashita
Mika
M
Sato
Hajime
H
Nakagawa
Yasuyoshi
Y
Tahara
Yasutaka
Y
D001407:Bacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics; D044822:Biodiversity; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D007887:Fabaceae / Q000382:microbiology*; D005285:Fermentation; D005516:Food Microbiology; D020459:Genes, rRNA; D005869:Ghana; D008969:Molecular Sequence Data; D020653:Peptide Elongation Factor G / Q000235:genetics; D010802:Phylogeny; D011099:Polyglutamic Acid / Q000096:biosynthesis* / Q000302:isolation & purification; D012319:RNA Polymerase II / Q000235:genetics; D012329:RNA, Bacterial / Q000235:genetics; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012689:Sequence Homology, Nucleic Acid
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D020653:Peptide Elongation Factor G; D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S; D011099:Polyglutamic Acid; D012319:RNA Polymerase II