A convenient one-step synthesis of L-aminotryptophans and improved synthesis of 5-fluorotryptophan.
A one-pot biotransformation for the generation of a series of L-aminotryptophans using a readily prepared protein extract containing tryptophan synthase is reported. The extract exhibits remarkable stability upon freeze-drying, and may be stored and used for long periods after its preparation without significant loss of activity.
Winn
Michael
M
School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, UK.
Roy
Abhijeet Deb
AD
Grüschow
Sabine
S
Parameswaran
Raj S
RS
Goss
Rebecca J M
RJ
D001671:Biochemistry / Q000379:methods*; D001711:Biotransformation; D002625:Chemistry, Organic / Q000379:methods; D004339:Drug Compounding; D015195:Drug Design; D004355:Drug Stability; D005612:Freeze Drying; D008956:Models, Chemical; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D014367:Tryptophan Synthase / Q000737:chemistry
C026927:5-fluorotryptophan; D014364:Tryptophan; D014367:Tryptophan Synthase