Online structural elucidation of alkaloids and other constituents in crude extracts and cultured cells of Nandina domestica by combination of LC-MS/MS, LC-NMR, and LC-CD analyses.
The combination of NMR, MS, and CD data permitted the structural elucidation including the absolute configuration of the known alkaloids and unknown components in the extract matrix solution of Nandina domestica without isolation and sample purification prior to the coupling experiments. Unstable natural stereoisomers were identified by LC-NMR and LC-MS. Five known alkaloids, (S)-isoboldine, (S)-domesticine, (S)-nantenine, sinoacutine, and menispermine, were identified from N. domestica. O-Methylpallidine and (E, E)-, (E, Z)-, and (Z, Z)-terrestribisamide were also characterized for the first time from this plant. Known jatrorrhizine, palmatine, and berberine and unknown (R)-carnegine and (E, E)-, (E, Z)-, and (Z, Z)-terrestribisamide were identified in the callus of N. domestica.
Iwasa
Kinuko
K
Kobe Pharmaceutical University, 4-19-1 Motoyamakita, Higashinada-ku, Kobe-shi 658-8558, Japan. k-iwasa@kobepharma-u.ac.jp
Takahashi
Teturo
T
Nishiyama
Yumi
Y
Moriyasu
Masataka
M
Sugiura
Makiko
M
Takeuchi
Atsuko
A
Tode
Chisato
C
Tokuda
Harukuni
H
Takeda
Kazuyoshi
K
D000470:Alkaloids / Q000737:chemistry*; D028141:Berberidaceae / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods*; D002942:Circular Dichroism / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry / Q000379:methods*
D000470:Alkaloids; D010936:Plant Extracts