Oocyte cryopreservation: the birth of the first Hungarian babies from frozen oocytes.
OBJECTIVE
To present data obtained with clinical application of oocyte cryopreservation.


METHODS
Slow freezing/rapid thawing in PBS based medium containing 1.5 M propanediol + 0.3 M sucrose.


RESULTS
A total of 127 embryos were transferred into 54 patients (1.9 embryo/cycle, 64 transfer cycles). Clinical pregnancy rate of 20% per cycle (13/64) and 24.0% per patient were achieved. Up-to-date, six patients delivered seven healthy babies; there are four ongoing pregnancies. Three abortions (23%) and one biochemical pregnancy (0.7%) was obtained. Implantation rates of 11% per transferred embryos (14/127) and 6.5% (14/215) per thawed eggs were found. In each case, normal karyotype was detected. No difference was found in the ratio of spindle positive oocytes at the polscope analyses done before and after freezing (75.8% vs. 82.5%).


CONCLUSIONS
Egg freezing is not a routine procedure yet, but there will certainly be a place for it in the future of assisted reproductive medicine.
Konc
Janos
J
Infertility and IVF Center of Buda, Saint János Hospital, Diósárok u. 1-3, Budapest, Hungary.
Kanyo
Katalin
K
Varga
Erika
E
Kriston
Rita
R
Cseh
Sandor
S
D000328:Adult; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology; D046149:Embryo Culture Techniques; D004624:Embryo Transfer / Q000379:methods; D005260:Female; D005615:Freezing; D006801:Humans; D006814:Hungary; D007621:Karyotyping; D008875:Middle Aged; D009865:Oocytes / Q000166:cytology* / Q000378:metabolism; D011247:Pregnancy; D018873:Pregnancy Rate; D013395:Sucrose / Q000737:chemistry
D003451:Cryoprotective Agents; D013395:Sucrose