The role of CD11b in phagocytosis and dendritic cell development.
Activation of resting T cells is highly dependent on dendritic cells (DCs), which take up antigens and present antigenic peptides to T cells in the context of the major histocompatibility complex (MHC). In this study, we generated a monoclonal antibody, which we call 1C4 that recognizes integrin alpha(M)beta(2) (CD11b/CD18) on the surface of conventional DCs (cDCs) and is internalized after binding. Addition of 1C4 inhibited the ability of immature DCs to phagocytose apoptotic cells. 1C4 treatment also partially inhibited the generation of cDCs from bone marrow in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF). Our findings suggest that not only CD11b is involved in the phagocytosis of apoptotic cells, but also that mAb such as 1C4 may be a useful tool for the delivery of specific proteins into the cytoplasm of immature DCs.
Chen
Jingtao
J
Department of Immunobiology, SBI Biotech Co. Ltd., Ginkgo Biomedical Research Institute, Tokyo, Japan.
Namiki
Sahori
S
Toma-Hirano
Makiko
M
Miyatake
Shoichiro
S
Ishida
Koji
K
Shibata
Yasue
Y
Arai
Naoko
N
Arai
Ken-Ichi
K
Kamogawa-Schifter
Yumiko
Y
D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D017209:Apoptosis / Q000276:immunology; D039481:CD11b Antigen / Q000276:immunology*; D002452:Cell Count; D002454:Cell Differentiation / Q000276:immunology*; D003713:Dendritic Cells / Q000166:cytology*; D005260:Female; D016177:Macrophage-1 Antigen / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010587:Phagocytosis / Q000276:immunology*; D051381:Rats; D017208:Rats, Wistar
D000911:Antibodies, Monoclonal; D039481:CD11b Antigen; D016177:Macrophage-1 Antigen