title: Isoflavonoid composition of a callus culture of the relict tree Maackia amurensis Rupr. et Maxim.
abstract: Isoflavonoids, an interesting and restricted group of secondary metabolites of legumes, exhibit estrogenic, antiangiogenic, and anticancer activities and are now popular as dietary supplements. Plant cell cultures that possess an increased ability to synthesize these metabolites were examined. During the investigation, cell cultures of the Far Eastern relict tree Maackia amurensis (Leguminosae) were established. A selection of seed-derived cell aggregates yielded the callus line designated A-18. This culture produces 20 isoflavonoids, namely, the isoflavones genistein, daidzein, formononetin, calycosin, derrone, and pseudobaptigenin and their glycosylated conjugates genistin, 6''-O-malonylgenistin, ononin, 6''-O-malonylononin, daidzin, 3'-methoxydaidzin, 4'-O-beta-D-glucopyranosyldaidzin, 4'-O-beta-D-glucopyranosylgenistin, and 7-O-beta-D-glucopyranosylcalycosin; the pterocarpans maackiain and medicarpin and their glycosylated conjugates 6'-O-malonyl-3-O-beta-D-glucopyranosylmaackiain and 6'-O-malonyl-3-O-beta-D-glucopyranosylmedicarpin; and the new pterocarpan glucoside 6'-O-malonyl-3-O-beta-D-glucopyranosyl-6,6a-dehydromaackiain. These isoflavonoids, possessing a hepatoprotective activity, were stably produced by the A-18 calli for prolonged periods of observation.
authors.affiliation: Pacific Institute of Bioorganic Chemistry, Far East Branch of Russian Academy of Sciences, Vladivostok, Russian Federation.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Fedoreyev
authors.forename: Victor P
authors.initials: VP
authors.lastname: Bulgakov
authors.forename: Olga V
authors.initials: OV
authors.lastname: Grishchenko
authors.forename: Marina V
authors.initials: MV
authors.lastname: Veselova
authors.forename: Olga E
authors.initials: OE
authors.lastname: Krivoschekova
authors.forename: Nadezda I
authors.initials: NI
authors.lastname: Kulesh
authors.forename: Vladimir A
authors.initials: VA
authors.lastname: Denisenko
authors.forename: Galina K
authors.initials: GK
authors.lastname: Tchernoded
authors.forename: Yury N
authors.initials: YN
authors.lastname: Zhuravlev
mesh_terms: D002460:Cell Line; D002851:Chromatography, High Pressure Liquid; D006031:Glycosylation; D007529:Isoflavones / Q000032:analysis* / Q000096:biosynthesis / Q000737:chemistry; D029901:Maackia / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D036226:Seedlings / Q000737:chemistry* / Q000166:cytology / Q000378:metabolism
chemical_list: D007529:Isoflavones