Establishment and characterization of a cell line (IGSK-3) secreting human chorionic gonadotropin, adrenocorticotropic hormone and parathyroid hormone-related protein derived from primary poorly differentiated adenocarcinoma of the stomach.
We recently established human chorionic gonadotropin-, adrenocorticotropic hormone- and parathyroid hormone-related protein-secreting cell line derived from primary poorly differentiated adenocarcinoma of the stomach. The cell line was designated as IGSK-3. Inverted-phase contrast microscopy revealed that the IGSK-3 cells consist of two morphological subtypes. One type has visible nucleoli and clear nuclei, but nucleoli and nuclear membrane of the other type are invisible. The population-doubling time was about 43 h. An analysis of conditioned medium by IGSK-3 cells cultured for 4 days revealed the IGSK-3 cells secrete human chorionic gonadotropin-beta (0.5 ng/mL), adrenocorticotropic hormone (5.5 pg/mL), parathyroid hormone-related protein (3.4 pmol/mL) and epidermal growth factor (14.2 pg/mL). Histopathological diagnosis of the graft of IGSK-3 cells revealed that IGSK-3 cells built a poorly differentiated adenocarcinoma which resembled the original tumor. In addition, the IGSK-3 cell line was immunocytochemically positive for human chorionic gonadotropin-beta and epidermal growth factor receptor, and negative for vascular endothelial growth factor.
Yamashita
Shigeo
S
Department of Surgery, Jikei University School of Medicine, Tokyo,105-8461, Japan.
Takahashi
Naoto
N
Hashimoto
Hisashi
H
Tachibana
Toshiaki
T
Nakahara
Taka
T
Ohyama
Akihiro
A
Yanaga
Katsuhiko
K
D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D000324:Adrenocorticotropic Hormone / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D006063:Chorionic Gonadotropin / Q000378:metabolism*; D005260:Female; D006801:Humans; D007621:Karyotyping; D051379:Mice; D008819:Mice, Nude; D008858:Microscopy, Phase-Contrast; D008875:Middle Aged; D009368:Neoplasm Transplantation; D044162:Parathyroid Hormone-Related Protein / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000648:ultrastructure; D014183:Transplantation, Heterologous
D006063:Chorionic Gonadotropin; D044162:Parathyroid Hormone-Related Protein; D000324:Adrenocorticotropic Hormone