[Role of c-jun in the effect of genistein on neonatal rat calvaria osteoblasts proliferation and differentiation].
OBJECTIVE
To study the role of the c-jun in the effects of genistein on osteoblasts in neonatal rat calvaria cultures in vitro.


METHODS
Osteoblasts were isolated from neonatal rat calvaria through trypsin and collagenase digestion, and cultured in the medium at the doses of 10(-5), 10(-6) and 10(-7) mol/L genistein and at the dose of 10(-9) mol/L and 10(-10) mol/L estrogen for 48h and 72h. The proliferation and DNA of osteoblasts were assayed by MTT method and H-TdR incorporation, and c-jun was measured by in situ hybridization assay.


RESULTS
Genistein significantly promoted osteoblast proliferation and DNA synthesis. The values of MTT in control and genistein and estrogen groups at 48h and 72h were 0.19, 0.15, and 0.39, 0.45, 0.46, and 0.29, 0.32, 0.37 and 0.35, 0.38, and 0.50, 0.49. The content of 3H-TdR incorporation in control and genistein and estrogen groups were 68.47, and 101, 844, 512 and 1108.8, 1204.2. There was no difference of expression of c-jun among control and genistein and estrogen groups.


CONCLUSIONS
Genistein could promoted osteoblast proliferation and differentiation not associated with c-jun.
Zhang
Yuehong
Y
Department of Nutrition, General Hospital of PLA, Beijing 100853, China. zhangyh680@sohu.com
Jin
Hong
H
Xu
Zhiqin
Z
Nan
Wenkao
W
D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D019833:Genistein / Q000494:pharmacology*; D010006:Osteoblasts / Q000166:cytology*; D016755:Proto-Oncogene Proteins c-jun / Q000378:metabolism; D051381:Rats; D012886:Skull / Q000166:cytology*
D016755:Proto-Oncogene Proteins c-jun; D019833:Genistein