SPE and HPLC/UV of resin acids in colophonium-containing products.
A new method, involving SPE and HPLC/UV diode-array detection (DAD), was developed for the quantification of colophonium components in different consumer products, such as cosmetics. Colophonium is a common cause of contact dermatitis since its components can oxidize into allergens on exposure to air. Three different resin acids were used as markers for native and oxidized colophonium, abietic acid (AbA), dehydroabietic acid (DeA), and 7-oxodehydroabietic acid (7-O-DeA). The SPE method, utilizing a mixed-mode hydrophobic and anion exchange retention mechanism, was shown to yield very clean extracts. The use of a urea-embedded C(12) HPLC stationary phase improved the separation of the resin acids compared to common C(18). Concentrations higher than 2 mg/g of both AbA and DeA were detected in wax strips. In this product also 7-O-DeA, a marker for oxidized colophonium, was detected at a level of 28 microg/g. The LODs were in the range of 7-19 microg/g and the LOQs 22-56 microg/g. The method is simple to use and can be applied on many types of technical products, not only cosmetics. For the first time, a method for technical products was developed, which separates AbA from pimaric acid.
Nilsson
Ulrika
U
Department of Analytical Chemistry, Stockholm University, Stockholm, Sweden. ulrika.nilsson@anchem.su.se
Berglund
Naghmeh
N
Lindahl
Fredrik
F
Axelsson
Sara
S
Redeby
Theres
T
Lassen
Pia
P
Karlberg
Ann-Therese
AT
D045784:Abietanes / Q000032:analysis* / Q000138:chemical synthesis; D000040:Abscisic Acid / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods; D004983:Ethanolamines / Q000032:analysis*; D008968:Molecular Conformation; D012116:Resins, Plant / Q000032:analysis* / Q000737:chemistry*; D012680:Sensitivity and Specificity; D052616:Solid Phase Extraction / Q000295:instrumentation / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet / Q000295:instrumentation / Q000379:methods; D013237:Stereoisomerism
D045784:Abietanes; D004983:Ethanolamines; D012116:Resins, Plant; D000040:Abscisic Acid; C013893:rosin; C020283:diethanolamine; C095375:7-oxodehydroabietic acid