title: Online structural elucidation of alkaloids and other constituents in crude extracts and cultured cells of Nandina domestica by combination of LC-MS/MS, LC-NMR, and LC-CD analyses.
abstract: The combination of NMR, MS, and CD data permitted the structural elucidation including the absolute configuration of the known alkaloids and unknown components in the extract matrix solution of Nandina domestica without isolation and sample purification prior to the coupling experiments. Unstable natural stereoisomers were identified by LC-NMR and LC-MS. Five known alkaloids, (S)-isoboldine, (S)-domesticine, (S)-nantenine, sinoacutine, and menispermine, were identified from N. domestica. O-Methylpallidine and (E, E)-, (E, Z)-, and (Z, Z)-terrestribisamide were also characterized for the first time from this plant. Known jatrorrhizine, palmatine, and berberine and unknown (R)-carnegine and (E, E)-, (E, Z)-, and (Z, Z)-terrestribisamide were identified in the callus of N. domestica.
authors.affiliation: Kobe Pharmaceutical University, 4-19-1 Motoyamakita, Higashinada-ku, Kobe-shi 658-8558, Japan. k-iwasa@kobepharma-u.ac.jp
authors.forename: Kinuko
authors.initials: K
authors.lastname: Iwasa
authors.forename: Teturo
authors.initials: T
authors.lastname: Takahashi
authors.forename: Yumi
authors.initials: Y
authors.lastname: Nishiyama
authors.forename: Masataka
authors.initials: M
authors.lastname: Moriyasu
authors.forename: Makiko
authors.initials: M
authors.lastname: Sugiura
authors.forename: Atsuko
authors.initials: A
authors.lastname: Takeuchi
authors.forename: Chisato
authors.initials: C
authors.lastname: Tode
authors.forename: Harukuni
authors.initials: H
authors.lastname: Tokuda
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Takeda
mesh_terms: D000470:Alkaloids / Q000737:chemistry*; D028141:Berberidaceae / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D002853:Chromatography, Liquid / Q000379:methods*; D002942:Circular Dichroism / Q000379:methods*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D010936:Plant Extracts