Thermal oscillations in rat kidneys: an infrared imaging study.
A high-resolution infrared (IR) camera was used to assess rhythmicity in localized renal blood flow, including the extent of regions containing nephrons with spontaneous oscillations in their individual blood flow. The IR imaging was able to follow changes in rat renal perfusion during baseline conditions, during occlusion of the main renal artery and during the administration of either saline or papaverine. Concurrent recordings were made of tubular pressure in superficial nephrons. Spontaneous vascular oscillations centred around 0.02-0.05 Hz and approximately 0.01 Hz could be detected reproducibly by IR imaging. Their spectral characteristics and their response to papaverine were in line with tubular pressure measurements. The intensity of and synchrony between thermal signals from different local areas of the kidney may allow, after surgical exposure, non-invasive imaging of functional clusters involved in renal cortical blood flow. Through visualization of the spatial extent of thermal oscillations, IR imaging holds promise in assessing kidney autoregulatory mechanisms.
Gorbach
Alexander M
AM
National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Building 13, Room 3N-11, Bethesda, MD 20892-5766, USA. gorbach@helix.nih.gov
Wang
Hengliang
H
Elster
Eric
E
D000818:Animals; D001703:Biophysics / Q000379:methods; D002138:Calibration; D016000:Cluster Analysis; D007668:Kidney / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology*; D008853:Microscopy / Q000379:methods; D015233:Models, Statistical; D008962:Models, Theoretical; D009399:Nephrons / Q000473:pathology*; D009991:Oscillometry / Q000379:methods; D010477:Perfusion; D051381:Rats; D012077:Renal Artery / Q000473:pathology; D013055:Spectrophotometry, Infrared / Q000379:methods*; D013696:Temperature