Localization of serotonin transporter mRNA in the CNS of Lymnaea stagnalis.
The serotonin transporter, SERT, is reported as a key molecule that regulates serotonergic neurotransmission. In the present study, we analyzed the localization of Lymnaea SERT (LymSERT) mRNA-containing neurons by in situ hybridization using frozen sections of the central nervous system (CNS) of Lymnaea. To precisely demonstrate the distribution of LymSERT mRNA-containing neurons, colocalization with serotonin immunoreactivity was also examined. The results showed that LymSERT mRNA was constitutively expressed and localized in the serotonin-containing neurons in the CNS.
Sadamoto
Hisayo
H
Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa 769-2193, Japan. sadamotoh@kph.bunri-u.ac.jp
Serfozo
Z
Z
Ito
E
E
D000818:Animals; D001483:Base Sequence; D002490:Central Nervous System / Q000378:metabolism; D018076:DNA, Complementary / Q000235:genetics; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D008195:Lymnaea / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012701:Serotonin / Q000378:metabolism; D050486:Serotonin Plasma Membrane Transport Proteins / Q000235:genetics*
D018076:DNA, Complementary; D012333:RNA, Messenger; D050486:Serotonin Plasma Membrane Transport Proteins; D012701:Serotonin