title: Loss of peroxisome function triggers necrosis.
abstract: Disturbance of peroxisome function can lead to various degenerative diseases during ageing. Here, we show that in yeast deletion of PEX6, encoding a protein involved in a key step of peroxisomal protein import, results in an increased accumulation of reactive oxygen species and an enhanced loss of viability upon acetic acid treatment and during early stationary phase. Cell death of ageing-like yeast cells lacking PEX6 does not depend on the apoptotic key players Yca1p and Aif1p, but instead shows markers of necrosis. Thus, we conclude that loss of peroxisomal function leads to a form of necrotic cell death.
authors.affiliation: Institute of Molecular Biosciences (IMB), University of Graz, Austria.
authors.forename: Helmut
authors.initials: H
authors.lastname: Jungwirth
authors.forename: Julia
authors.initials: J
authors.lastname: Ring
authors.forename: Tanja
authors.initials: T
authors.lastname: Mayer
authors.forename: Alexandra
authors.initials: A
authors.lastname: Schauer
authors.forename: Sabrina
authors.initials: S
authors.lastname: Büttner
authors.forename: Tobias
authors.initials: T
authors.lastname: Eisenberg
authors.forename: Didac
authors.initials: D
authors.lastname: Carmona-Gutierrez
authors.forename: Karl
authors.initials: K
authors.lastname: Kuchler
authors.forename: Frank
authors.initials: F
authors.lastname: Madeo
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D019342:Acetic Acid / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000502:physiology*; D017209:Apoptosis / Q000235:genetics; D020169:Caspases / Q000378:metabolism; D017353:Gene Deletion; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009336:Necrosis / Q000235:genetics*; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; C443623:AIF1 protein, S cerevisiae; D009247:NADH, NADPH Oxidoreductases; D020169:Caspases; C456272:MCA1 protein, S cerevisiae; D000251:Adenosine Triphosphatases; D000074183:ATPases Associated with Diverse Cellular Activities; C490760:PEX6 protein, S cerevisiae; D019342:Acetic Acid