Small-interference RNA-mediated knock-down of aldehyde oxidase 1 in 3T3-L1 cells impairs adipogenesis and adiponectin release.
Aldehyde oxidase 1 (AOX1) is highly abundant in the liver and oxidizes aldehydes thereby generating reactive oxygen species. Enzymes involved in detoxification of aldehydes are expressed in adipocytes and alter adipogenesis, therefore the functional role of AOX1 in adipocytes was analyzed. AOX1 mRNA was higher in visceral compared to subcutaneous human adipose tissue but AOX1 protein was detected in both fat depots. AOX1 expression in adipocytes was confirmed by immunohistochemistry and immunoblot. AOX1 was induced during adipocytic differentiation and was downregulated by fenofibrate in differentiated cells. Knock-down of AOX1 in preadipocytes led to impaired lipid storage and adiponectin release in the differentiated cells. These data indicate that AOX1 is essential for adipogenesis and may link energy and drug metabolism.
Weigert
Johanna
J
Department of Internal Medicine I, Regensburg University Hospital, Regensburg, Germany.
Neumeier
Markus
M
Bauer
Sabrina
S
Mages
Wolfgang
W
Schnitzbauer
Andreas A
AA
Obed
Aiman
A
Gröschl
Benedikt
B
Hartmann
Arndt
A
Schäffler
Andreas
A
Aslanidis
Charalampos
C
Schölmerich
Jürgen
J
Buechler
Christa
C
D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000201:enzymology*; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics*; D052242:Adiponectin / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000502:physiology*; D000818:Animals; D011345:Fenofibrate / Q000494:pharmacology; D051379:Mice; D000077205:Pioglitazone; D034741:RNA, Small Interfering / Q000235:genetics; D045162:Thiazolidinediones / Q000494:pharmacology
D052242:Adiponectin; D034741:RNA, Small Interfering; D045162:Thiazolidinediones; D000445:Aldehyde Oxidoreductases; C443116:Aox3 protein, mouse; D011345:Fenofibrate; D000077205:Pioglitazone