[Construction of the male rat model of adenine-induced infertility with kidney-yang deficiency].
OBJECTIVE
To investigate the time- and dose-related reproductive toxicity of adenine in male Wistar rats.


METHODS
Adenine was dissolved with Arabian sol at the proportion of 1:10 and different adenine solutions were prepared at the concentration of 0, 50, 100, 150 and 200 mg/ml. Seventy-five male Wistar rats were equally assigned to 5 dose groups and received intragastric administration of the adenine solution at 1 ml/(100 g x d). Five from each group were sacrificed every 10, 20 and 30 days, their sperm quality and body and genital weight measured, pathological examinations conducted, sex hormone changes detected by radioimmunoassay, and analyses made on the time- and dose-related reproductive toxicity of adenine.


RESULTS
With the increase in the time and dose of adenine administration, signs of kidney-yang deficiency gradually appeared in all the dose groups on the third day; and statistically significant changes were observed in sperm concentration and motility, and serum testosterone and luteinizing hormone levels in the 100 mg/(100 g x d) group (P < 0.05 or P < 0.01) by the 10th day. The same changes were observed in 50 mg/(100 g x d) group by the 20th day. Different degrees of pathological changes were noted in a time and dose-dependent manner in all the dose groups, suggestive of a progressive reduction of the reproductive function with the increase of time and dose of adenine administration.


CONCLUSIONS
For the construction of the male rat model of adenine-induced infertility with kidney-yang deficiency, the best dose is 50-100 mg/(100 g x d) and the best administration time is 10-20 consecutive days intragastrically.
Wang
Jia-hui
JH
Department of Urology and Reproduction, the Second Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110034, China.
Chen
Dong
D
Zhou
Jian-guo
JG
Cao
Yong-gang
YG
Jia
Jin-ming
JM
D000225:Adenine / Q000633:toxicity*; D000818:Animals; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007248:Infertility, Male / Q000097:blood / Q000139:chemically induced* / Q000473:pathology; D007986:Luteinizing Hormone / Q000097:blood; D008297:Male; D011863:Radioimmunoassay; D051381:Rats; D017208:Rats, Wistar; D013739:Testosterone / Q000097:blood; D013997:Time Factors; D016711:Yang Deficiency / Q000097:blood / Q000139:chemically induced* / Q000473:pathology
D013739:Testosterone; D007986:Luteinizing Hormone; D000225:Adenine