[Genetic diversity of eukarytic microplankton in different areas of Lake Taihu].
The methods of DGGE and cloning/sequencing were used to study the diversity and community structures of small planktons (0.8 - 20 microm) in different areas of Lake Taihu. DGGE indicated that there were markly various fingerprints in different areas and the diversities were higher in areas with low trophic status than those with relatively high trophic status. There were 23, 24 DGGE bands in East Taihu and Gonghu Bay, respectively (Shannon index were 3.135 and 3.178) and 18 bands in both Meiliang Bay and Wuli Bay (Both shannon index were 2.890). The result of cloning/sequencing indicated that there was a high diversity of small planktons in Lake Taihu and most of them phototrophic flagellate, heterotrophic flagellate, ciliate and fungi. There were various community structures in the three different clone libraries. In Meilang Bay, 28.6% OTUs(operational taxonomic unit)belonged to heterotrophic flagellate, followed by Cryptophyta (22.9%) and Chrysophyta (14.3%). In Central Lake, 25.7% OTUs belonged to Chrysophyta, followed by heterotrophic flagellate (20.0%) and Cryptophyta (14.3 %). In East Taihu, ciliates were the dominant group and only a few heterotrophic flagellates (40.9%) were detected. In addition, fungi were relatively abundant (12.2%) in this area.
Chen
Mei-Jun
MJ
State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210008, China. meijunch@163.com
Kong
Fan-Xiang
FX
Chen
Fei-Zhou
FZ
Xing
Peng
P
D000818:Animals; D002681:China; D016000:Cluster Analysis; D004784:Environmental Monitoring; D005068:Eutrophication; D005618:Fresh Water / Q000032:analysis*; D014644:Genetic Variation; D005843:Geography; D010839:Phytoplankton / Q000235:genetics / Q000254:growth & development; D010933:Plankton / Q000145:classification / Q000235:genetics* / Q000254:growth & development; D016133:Polymerase Chain Reaction; D012150:Polymorphism, Restriction Fragment Length; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D017422:Sequence Analysis, DNA; D015048:Zooplankton / Q000235:genetics
D012337:RNA, Ribosomal, 18S