Mutant amyloid-beta-sensitized dendritic cells as Alzheimer's disease vaccine.
Vaccines using bone marrow-derived dendritic cells (DCs) sensitized to Abeta 1-42 peptide and other mutant peptides were tested on BALB/c and APP(SW) transgenic mice. Wild type Abeta 1-42-sensitized DC vaccine (DCSV) produced no response, but all peptides with a T-cell epitope mutation induced antibody responses without inflammation. DCSV with Abeta 1-25 peptide with mutated T-cell epitope failed to induce antibody response, while DCSV with Abeta 1-35 with mutated T-cell epitope produced a strong antibody response. The entire T-cell epitope is required in a DC vaccine to induce antibody response. DCSV with Abeta peptide carrying the entire mutant T-cell epitope may be an appropriate vaccine against AD.
Cao
Chuanhai
C
Johnnie B. Byrd, Sr., Alzheimer's Center and Research Institute, 4001 East Fletcher Avenue, Tampa, FL 33613, United States. ccao@byrdinstitute.org
Lin
Xiaoyang
X
Zhang
Chi
C
Wahi
Monika M
MM
Wefes
Inge
I
Arendash
Gary
G
Potter
Huntington
H
D023582:Alzheimer Vaccines / Q000276:immunology*; D016229:Amyloid beta-Peptides / Q000235:genetics* / Q000276:immunology* / Q000494:pharmacology; D016564:Amyloid beta-Protein Precursor / Q000235:genetics; D000818:Animals; D000917:Antibody Formation / Q000187:drug effects / Q000235:genetics; D000918:Antibody Specificity / Q000187:drug effects / Q000235:genetics; D001853:Bone Marrow / Q000033:anatomy & histology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D018604:Epitope Mapping; D005260:Female; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D009154:Mutation; D010446:Peptide Fragments / Q000235:genetics / Q000494:pharmacology; D013997:Time Factors
D023582:Alzheimer Vaccines; D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D010446:Peptide Fragments; C075222:amyloid beta-protein (1-42)