title: Increased levels of a particular phosphatidylcholine species in senescent human dermal fibroblasts in vitro.
abstract: Plasma membranes are essential components of living cells, and phospholipids are major components of cellular membranes. Here, we used liquid chromatography/mass spectrometry to investigate changes in the membrane phospholipid content that occur in association with aging. Our results indicate that the levels of a particular species of phosphatidylcholine comprised of stearic acid and arachidonic acid increased with age. To determine the reason for the increased levels of this particular phosphatidylcholine, we examined the effect of highly unsaturated fatty acids, such as arachidonic acid and eicosapentaenoic acid, on cellular aging. Applied arachidonic acid was incorporated into phosphatidylcholine molecules, but neither arachidonic acid nor other related unsaturated fatty acids had any effect. We conclude that increased levels of this distinctive phosphatidylcholine are a result of in vitro senescence.
authors.affiliation: Research and Development Division, KOSE Corporation, Tokyo, 114-0005, Japan. e-naru@kose.co.jp
authors.forename: Eiji
authors.initials: E
authors.lastname: Naru
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Takanezawa
authors.forename: Misako
authors.initials: M
authors.lastname: Kobayashi
authors.forename: Yuko
authors.initials: Y
authors.lastname: Misaki
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Kaji
authors.forename: Kumi
authors.initials: K
authors.lastname: Arakane
mesh_terms: D000375:Aging / Q000378:metabolism*; D016718:Arachidonic Acid / Q000378:metabolism / Q000494:pharmacology; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D002853:Chromatography, Liquid; D020405:Dermis / Q000166:cytology*; D015118:Eicosapentaenoic Acid / Q000378:metabolism / Q000494:pharmacology; D005347:Fibroblasts / Q000378:metabolism*; D006801:Humans; D013058:Mass Spectrometry; D010713:Phosphatidylcholines / Q000378:metabolism*
chemical_list: D010713:Phosphatidylcholines; D016718:Arachidonic Acid; D015118:Eicosapentaenoic Acid