title: Human fasting plasma concentrations of vitamin E and carotenoids, and their association with genetic variants in apo C-III, cholesteryl ester transfer protein, hepatic lipase, intestinal fatty acid binding protein and microsomal triacylglycerol transfer protein.
abstract: Plasma concentrations of vitamin E and carotenoids are governed by several factors, including genetic factors. Single nucleotide polymorphisms (SNP) in some genes involved in lipid metabolism have recently been associated with fasting plasma concentrations of these fat-soluble micronutrients. To further investigate the role of genetic factors that modulate the plasma concentrations of these micronutrients, we assessed whether SNP in five candidate genes (apo C-III, CETP, hepatic lipase, I-FABP and MTP) were associated with the plasma concentrations of these micronutrients. Fasting plasma vitamin E and carotenoid concentrations were measured in 129 French Caucasian subjects (forty-eight males and eighty-one females). Candidate SNP were genotyped by PCR amplification followed by restriction fragment length polymorphisms. Plasma gamma-tocopherol, alpha-carotene and beta-carotene concentrations were significantly different (P < 0.05) in subjects who carried different SNP variants in hepatic lipase. Plasma alpha-tocopherol concentrations were significantly different in subjects who had different SNP variants in apo C-III and cholesteryl ester transfer protein (CETP). Plasma lycopene concentrations were significantly different (P < 0.05) in women who had different SNP variants in intestinal fatty acid binding protein (I-FABP). Finally, there was no effect of SNP variants in microsomal TAG transfer protein upon the plasma concentrations of these micronutrients. Most of the observed differences remained significant after the plasma micronutrients were adjusted for plasma TAG and cholesterol. These results suggest that apo C-III, CETP and hepatic lipase play a role in determining the plasma concentrations of tocopherols while hepatic lipase and I-FABP may modulate plasma concentrations of carotenoids.
authors.affiliation: INRA, UMR 1260 Nutriments Lipidiques et Prévention des Maladies Métaboliques, Marseille, France. patrick.borel@univmed.fr
authors.forename: Patrick
authors.initials: P
authors.lastname: Borel
authors.forename: Myriam
authors.initials: M
authors.lastname: Moussa
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Reboul
authors.forename: Bernard
authors.initials: B
authors.lastname: Lyan
authors.forename: Catherine
authors.initials: C
authors.lastname: Defoort
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Vincent-Baudry
authors.forename: Matthieu
authors.initials: M
authors.lastname: Maillot
authors.forename: Marguerite
authors.initials: M
authors.lastname: Gastaldi
authors.forename: Michel
authors.initials: M
authors.lastname: Darmon
authors.forename: Henri
authors.initials: H
authors.lastname: Portugal
authors.forename: Denis
authors.initials: D
authors.lastname: Lairon
authors.forename: Richard
authors.initials: R
authors.lastname: Planells
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D053305:Apolipoprotein C-III / Q000235:genetics; D002338:Carotenoids / Q000097:blood*; D002352:Carrier Proteins / Q000235:genetics; D002784:Cholesterol / Q000097:blood; D053480:Cholesterol Ester Transfer Proteins / Q000097:blood; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D050556:Fatty Acid-Binding Proteins / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D008049:Lipase / Q000235:genetics; D008297:Male; D018977:Micronutrients / Q000235:genetics*; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000502:physiology*; D014280:Triglycerides / Q000097:blood; D014810:Vitamin E / Q000097:blood*; D055815:Young Adult
chemical_list: D053305:Apolipoprotein C-III; C506642:CETP protein, human; D002352:Carrier Proteins; D053480:Cholesterol Ester Transfer Proteins; D050556:Fatty Acid-Binding Proteins; C504173:LIPC protein, human; D018977:Micronutrients; D014280:Triglycerides; C106092:microsomal triglyceride transfer protein; D014810:Vitamin E; D002338:Carotenoids; D002784:Cholesterol; D008049:Lipase