Inhibitory action of soybean beta-conglycinin hydrolysates on Salmonella typhimurium translocation in Caco-2 epithelial cell monolayers.
Soybean protein hydrolysates are widely used as functional foods as they have antioxidative properties able to enhance immune responses in humans. The alcalase enzymatic hydrolysates of beta-conglycinin were fractionated by ultrafiltration, and two main fractions, SP1 (<10 kDa) and SP2 (10-20 kDa), were obtained. The effects of these two fractions on the growth, development of epithelial cells, and formation of intercellular tight junctions were tested on an in vitro Caco-2 cell culture system. The inhibitory effects of SP1 and SP2 on the penetration of Salmonella typhimurium into Caco-2 epithelial cells were also examined. The results showed that the addition of >0.05 g/L of SP2 improved epithelial cell growth and that a concentration of 0.5 g/L of SP2 increased intercellular tight junction formation, which resulted in increased of transepithelial monolayer resistance (TER) values. Moreover, a lower S. typhimurium count compared to control was obtained when Caco-2 cells were grown in 0.05 and 0.5 g/L of SP2. These results show that beta-conglycinin hydrolysates play an important role in resisting S. typhimurium penetration into intestinal epithelial cells and that high molecular mass peptides (10-20 kDa) were more effective overall than low molecular mass peptides.
Yang
Baichong
B
College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghua Donglu, Haidian District, Beijing 100083, China.
Lv
Ying
Y
Chen
Yang
Y
Wang
Jin
J
Tang
Wuxia
W
Guo
Shuntang
S
D052179:Antigens, Plant; D018988:Bacterial Translocation / Q000187:drug effects*; D018938:Caco-2 Cells; D005916:Globulins / Q000378:metabolism / Q000494:pharmacology*; D006801:Humans; D006868:Hydrolysis; D007422:Intestines / Q000382:microbiology / Q000648:ultrastructure; D046529:Microscopy, Electron, Transmission; D012486:Salmonella typhimurium / Q000502:physiology*; D055314:Seed Storage Proteins; D030262:Soybean Proteins / Q000378:metabolism / Q000494:pharmacology*; D013025:Glycine max / Q000737:chemistry*; D013381:Subtilisins / Q000378:metabolism
D052179:Antigens, Plant; D005916:Globulins; D055314:Seed Storage Proteins; D030262:Soybean Proteins; C013894:beta-conglycinin protein, Glycine max; D013381:Subtilisins