[Growth of Methylosinus trichosporium OB3b on methane and poly-beta-hydroxybutyrate biosynthesis].
Optimal conditions for batch cultivation of the obligate methanotroph Methylosinus trichosporium OB3b on methane without superatmospheric pressure were chosen. The yield of absolutely dry biomass after 120 h of growth reached 20 g/l. This biomass contained 30% poly-beta-hydroxybutyrate (PHB) with molecular weight 300 kDa. The growth process included the stages of biomass growth and PHB biosynthesis. The latter stage occurred under nitrogen-deficiency conditions. It was accompanied by an increase in the activity of PHB biosynthesis enzymes (beta-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase) and the main NAD(P)H producer, methylenetetrahydromethanopterin dehydrogenase. The activity of PHB depolymerase increased insignificantly.
Doronina
N V
NV
Ezhov
V A
VA
Trotsenko
Iu A
IuA
D000102:Acetyl-CoA C-Acyltransferase / Q000378:metabolism; D000217:Acyltransferases / Q000378:metabolism; D000429:Alcohol Oxidoreductases / Q000378:metabolism; D018533:Biomass; D006885:Hydroxybutyrates / Q000378:metabolism*; D008697:Methane / Q000378:metabolism*; D020602:Methylosinus trichosporium / Q000254:growth & development / Q000378:metabolism*; D009584:Nitrogen / Q000378:metabolism; D000587:Oxidoreductases Acting on CH-NH Group Donors / Q000378:metabolism; D011091:Polyesters / Q000378:metabolism*; D000094122:Polyhydroxybutyrates
D000102:Acetyl-CoA C-Acyltransferase; D000217:Acyltransferases; D000429:Alcohol Oxidoreductases; D006885:Hydroxybutyrates; D008697:Methane; D009584:Nitrogen; D000587:Oxidoreductases Acting on CH-NH Group Donors; D011091:Polyesters; C003182:poly-beta-hydroxybutyrate; D000094122:Polyhydroxybutyrates; C023744:acetoacetyl-CoA reductase; C047126:methylenetetrahydromethanopterin dehydrogenase; C060970:poly-beta-hydroxybutyrate polymerase