Aldehyde-terminated amorphous carbon substrates for the fabrication of biomolecule arrays.
Amorphous carbon thin films are easily deposited at room temperature, readily functionalized with alkene-containing molecules through a UV photochemical reaction, and provide a robust surface capable of supporting chemical and biomolecule array fabrication. Aldehyde-terminated amorphous carbon substrates were fabricated via the attachment of a 2-(10-undecen-1-yl)-1,3-dioxolane molecule. The surfaces were then deprotected in 1.5 M HCl to yield an aldehyde-terminated surface that is readily reactive with amine containing molecules. An array of amine-modified oligonucleotides was prepared on aldehyde-terminated surfaces prepared on both amorphous carbon and on gold self-assembled monolayers, and the fluorescence background, feature signal-to-noise ratio, and hybridization densities were compared. The aldehyde-terminated amorphous carbon substrates offer inherently lower background fluorescence intensity and a greater number of hybridization-accessible sites.
Lockett
Matthew R
MR
Department of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison, Wisconsin 53706, USA.
Shortreed
Michael R
MR
Smith
Lloyd M
LM
D000447:Aldehydes / Q000737:chemistry*; D000588:Amines / Q000737:chemistry; D001483:Base Sequence; D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry*; D006851:Hydrochloric Acid / Q000737:chemistry; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D009841:Oligonucleotides / Q000737:chemistry; D010777:Photochemistry / Q000379:methods; D013055:Spectrophotometry, Infrared / Q000379:methods; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013997:Time Factors; D014466:Ultraviolet Rays
D000447:Aldehydes; D000588:Amines; D009841:Oligonucleotides; D002244:Carbon; D006851:Hydrochloric Acid