Nodal/Bozozok-independent induction of the dorsal organizer by zebrafish cell lines.
Formation of the dorsal organizer (Spemann organizer) is an important process in early vertebrate development. In zebrafish, two molecular cascades--Bozozok/Dharma (Boz) and Nodal signaling--act in parallel to induce the dorsal organizer. However, the complete molecular mechanism regulating this event remains unclear. Here we report that zebrafish cell lines derived from various developmental stages can induce a secondary axis when they are implanted into the mid-blastula but not the early gastrula. The implanted cells themselves did not differentiate, but instead induced ectopic expression of dorsal organizer markers in cells around the implanted cells and induced notochord formation in the secondary axis. These results indicate that cultured cell lines have the ability to induce a secondary axis through the initiation of dorsal organizer activity. However, ectopic expression of boz and sqt were not observed in cultured cells. In addition, implanted cell lines could induce the dorsal organizer even in maternal-zygotic one-eyed pinhead mutants, which are not responsive to Nodal signaling. Finally, the Nodal signaling pathway was not activated following implantation of cultured cells. Collectively, these data suggest that zebrafish cell lines induce the dorsal organizer independent of the boz and Nodal signaling pathways.
Hashiguchi
Megumi
M
Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI), Mishima 411-8540, Japan.
Shinya
Minori
M
Tokumoto
Mika
M
Sakai
Noriyoshi
N
D000818:Animals; D019521:Body Patterning / Q000502:physiology*; D002478:Cells, Cultured; D004627:Embryonic Induction / Q000502:physiology*; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism*; D017403:In Situ Hybridization; D009154:Mutation / Q000235:genetics; D055457:Nodal Protein; D020897:Organizers, Embryonic / Q000196:embryology* / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D015027:Zebrafish / Q000196:embryology*; D029961:Zebrafish Proteins / Q000235:genetics / Q000378:metabolism*
D018398:Homeodomain Proteins; D055457:Nodal Protein; D014157:Transcription Factors; D016212:Transforming Growth Factor beta; D029961:Zebrafish Proteins; C113874:dharma protein, zebrafish; C110484:tdgf1 protein, zebrafish