title: Mitochondrial dysfunction increases oxidative stress and decreases chronological life span in fission yeast.
abstract: BACKGROUND
  Oxidative stress is a probable cause of aging and associated diseases. Reactive oxygen species (ROS) originate mainly from endogenous sources, namely the mitochondria.
  
  
  RESULTS
  We analyzed the effect of aerobic metabolism on oxidative damage in Schizosaccharomyces pombe by global mapping of those genes that are required for growth on both respiratory-proficient media and hydrogen-peroxide-containing fermentable media. Out of a collection of approximately 2700 haploid yeast deletion mutants, 51 were sensitive to both conditions and 19 of these were related to mitochondrial function. Twelve deletion mutants lacked components of the electron transport chain. The growth defects of these mutants can be alleviated by the addition of antioxidants, which points to intrinsic oxidative stress as the origin of the phenotypes observed. These respiration-deficient mutants display elevated steady-state levels of ROS, probably due to enhanced electron leakage from their defective transport chains, which compromises the viability of chronologically-aged cells.
  
  
  CONCLUSIONS
  Individual mitochondrial dysfunctions have often been described as the cause of diseases or aging, and our global characterization emphasizes the primacy of oxidative stress in the etiology of such processes.
authors.affiliation: Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Alice
authors.initials: A
authors.lastname: Zuin
authors.forename: Natalia
authors.initials: N
authors.lastname: Gabrielli
authors.forename: Isabel A
authors.initials: IA
authors.lastname: Calvo
authors.forename: Sarela
authors.initials: S
authors.lastname: García-Santamarina
authors.forename: Kwang-Lae
authors.initials: KL
authors.lastname: Hoe
authors.forename: Dong Uk
authors.initials: DU
authors.lastname: Kim
authors.forename: Han-Oh
authors.initials: HO
authors.lastname: Park
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Hayles
authors.forename: José
authors.initials: J
authors.lastname: Ayté
authors.forename: Elena
authors.initials: E
authors.lastname: Hidalgo
mesh_terms: D002244:Carbon / Q000737:chemistry; D019069:Cell Respiration; D004272:DNA, Mitochondrial; D004583:Electrons; D017353:Gene Deletion; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008928:Mitochondria / Q000473:pathology*; D008954:Models, Biological; D009154:Mutation; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry; D010101:Oxygen Consumption; D017382:Reactive Oxygen Species; D012568:Schizosaccharomyces / Q000502:physiology*; D013997:Time Factors
chemical_list: D004272:DNA, Mitochondrial; D017382:Reactive Oxygen Species; D002244:Carbon; D006861:Hydrogen Peroxide; D010100:Oxygen