title: Effective induction of high-titer antibodies by viral vector vaccines.
abstract: Protein-in-adjuvant vaccines have shown limited success against difficult diseases such as blood-stage malaria. Here we show that a recombinant adenovirus-poxvirus prime-boost immunization regime (known to induce strong T cell immunogenicity) can also induce very strong antigen-specific antibody responses, and we identify a simple complement-based adjuvant to further enhance immunogenicity. Antibodies induced against a blood-stage malaria antigen by this viral vector platform are highly effective against Plasmodium yoelii parasites in mice and against Plasmodium falciparum in vitro.
authors.affiliation: The Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Headington, Oxford OX3 7DQ, UK. simon.draper@ndm.ox.ac.uk
authors.forename: Simon J
authors.initials: SJ
authors.lastname: Draper
authors.forename: Anne C
authors.initials: AC
authors.lastname: Moore
authors.forename: Anna L
authors.initials: AL
authors.lastname: Goodman
authors.forename: Carole A
authors.initials: CA
authors.lastname: Long
authors.forename: Anthony A
authors.initials: AA
authors.lastname: Holder
authors.forename: Sarah C
authors.initials: SC
authors.lastname: Gilbert
authors.forename: Fergal
authors.initials: F
authors.lastname: Hill
authors.forename: Adrian V S
authors.initials: AV
authors.lastname: Hill
mesh_terms: D000256:Adenoviridae / Q000737:chemistry; D000818:Animals; D005822:Genetic Vectors / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D008288:Malaria / Q000517:prevention & control; D017780:Malaria Vaccines / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000378:metabolism; D010967:Plasmodium yoelii / Q000378:metabolism; D011212:Poxviridae / Q000737:chemistry; D013601:T-Lymphocytes / Q000469:parasitology / Q000821:virology*; D014612:Vaccines / Q000737:chemistry; D022223:Vaccines, Subunit / Q000737:chemistry; D014765:Viral Vaccines / Q000737:chemistry*
chemical_list: D007074:Immunoglobulin G; D017780:Malaria Vaccines; D014612:Vaccines; D022223:Vaccines, Subunit; D014765:Viral Vaccines