title: Controlled encapsulation of single-cells into monodisperse picolitre drops.
abstract: Encapsulation of cells within picolitre-size monodisperse drops provides new means to perform quantitative biological studies on a single-cell basis for large cell populations. Variability in the number of cells per drop due to stochastic cell loading is a major barrier to these techniques. We overcome this limitation by evenly spacing cells as they travel within a high aspect-ratio microchannel; cells enter the drop generator with the frequency of drop formation.
authors.affiliation: Massachusetts General Hospital, BioMEMS Resource Center, Charlestown, MA, USA.
authors.forename: Jon F
authors.initials: JF
authors.lastname: Edd
authors.forename: Dino
authors.initials: D
authors.lastname: Di Carlo
authors.forename: Katherine J
authors.initials: KJ
authors.lastname: Humphry
authors.forename: Sarah
authors.initials: S
authors.lastname: Köster
authors.forename: Daniel
authors.initials: D
authors.lastname: Irimia
authors.forename: David A
authors.initials: DA
authors.lastname: Weitz
authors.forename: Mehmet
authors.initials: M
authors.lastname: Toner
mesh_terms: D018922:HL-60 Cells; D006801:Humans; D008832:Microchemistry / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*