Development of a selective sample clean-up method based on immuno-ultrafiltration for the determination of deoxynivalenol in maize.
This paper describes the development of a highly selective analytical method for the determination of deoxynivalenol (DON) in maize. The developed method is based on immuno-ultrafiltration (IUF) and is the first application of IUF as a clean-up strategy in food analysis. Quantification of DON was carried out by high-performance liquid chromatography with ultraviolet detection. In contrast to immunoaffinity chromatography, in IUF the antibodies are not bound to a solid support material but used in free form, thus making it possible to avoid the critical immobilisation step. Sample clean-up by IUF proved to be as selective as clean-up using commercially available immunoaffinity columns. The limit of detection (S/N=3) of the analytical method was found to be 74 ng DON/g maize. Repeated analysis of a certified maize reference material on four different days resulted in a mean recovery of 93% with a standard deviation of 10%.
Böhm
Christina
C
Department of Veterinary Public Health and Food Science, Institute of Nutrition, University of Veterinary Medicine, Veterinärplatz 1, A-1210 Vienna, Austria.
Cichna-Markl
Margit
M
Brenn-Struckhofova
Zdenka
Z
Razzazi-Fazeli
Ebrahim
E
D002846:Chromatography, Affinity; D002851:Chromatography, High Pressure Liquid; D005504:Food Analysis / Q000379:methods*; D009183:Mycotoxins / Q000032:analysis; D015203:Reproducibility of Results; D014255:Trichothecenes / Q000032:analysis*; D014462:Ultrafiltration / Q000379:methods; D003313:Zea mays / Q000737:chemistry*
D009183:Mycotoxins; D014255:Trichothecenes; C007262:deoxynivalenol