title: Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species.
abstract: By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.
authors.affiliation: Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Mariano
authors.initials: M
authors.lastname: Bossi
authors.forename: Jonas
authors.initials: J
authors.lastname: Fölling
authors.forename: Vladimir N
authors.initials: VN
authors.lastname: Belov
authors.forename: Vadim P
authors.initials: VP
authors.lastname: Boyarskiy
authors.forename: Rebecca
authors.initials: R
authors.lastname: Medda
authors.forename: Alexander
authors.initials: A
authors.lastname: Egner
authors.forename: Christian
authors.initials: C
authors.lastname: Eggeling
authors.forename: Andreas
authors.initials: A
authors.lastname: Schönle
authors.forename: Stefan W
authors.initials: SW
authors.lastname: Hell
mesh_terms: D000818:Animals; D002460:Cell Line; D003116:Color; D005456:Fluorescent Dyes / Q000737:chemistry*; D008853:Microscopy / Q000379:methods*; D015394:Molecular Structure
chemical_list: D005456:Fluorescent Dyes