title: [Expression of NR1 mRNA of NMDA receptor by gastrodine on hypoxia injury in cultured rat cerebral cortical neurons].
abstract: OBJECTIVE
  To investigate the effect of gastrodine on the expression of NR1 mRNA of NMDA receptor in cultured rat cerebral cortical neurons injury induced by hypoxia.
  
  
  METHODS
  The injury models of cultured rat cerebral cortical neurons was made by hypoxia and hypoglucose. The concentration of 13, 26, 52 mg x L(-1) of gastrodine was respectively added to the injured cells. The R-PCR technique was used to study the expression of NR1 mRNA of NMDA receptor.
  
  
  RESULTS
  The expression of NR1 mRNA increased markedly in hypoxia and hypoglucose injured cells, the increased expression can be weakened by gastrodine, more significant effect was found in the concentration of 26 mg x L(-1) 52 mg x L(-1).
  
  
  CONCLUSIONS
  Gastrodine may act as a neuro - protector by weakening the expression of NR1 mRNA in cultured rat cerebral cortical neurons injury induced by hypoxia and hypoglucose.
authors.affiliation: Pharmacy College, Zengzhou University, Zhengzhou 450001, China. oldfu123@zzu.edu.cn
authors.forename: Lei
authors.initials: L
authors.lastname: Fu
authors.forename: Yan-Hua
authors.initials: YH
authors.lastname: Mao
authors.forename: Yuan
authors.initials: Y
authors.lastname: Gao
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.forename: Zhi-Ping
authors.initials: ZP
authors.lastname: Wang
authors.forename: Liang-Chen
authors.initials: LC
authors.lastname: Li
mesh_terms: D000818:Animals; D001592:Benzyl Alcohols / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D000860:Hypoxia / Q000235:genetics*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D001592:Benzyl Alcohols; D005960:Glucosides; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; C045345:gastrodin