Micro-Raman analysis and AFM imaging of Acidithiobacillus ferrooxidans biofilm grown on uranium ore.
Acidithiobacillus ferrooxidans biofilm grown on uranium ore substrate was analyzed by a micro-Raman spectrometer and an atomic force microscope (AFM). The bacterium employed for this study, A. ferrooxidans BM1, was isolated from a uranium mine (Jaduguda, India). Micro-Raman analysis revealed the different constituents of molecular fragments present in microbial cells and in secreted extracellular polymeric substances (EPSs). AFM images clearly revealed bacterial cells surrounded by EPS. From Raman spectral data, the composition of EPS from A. ferrooxidans BM1 appeared to be similar to that of EPS secreted in a different Pseudomonas bacterium.
Pradhan
Nilotpala
N
Institute of Minerals and Materials Technology, Bhubaneswar 751013, Orissa, India. nilotpala_pradhan@yahoo.co.in
Pradhan
Siddhartha K
SK
Nayak
Bijan B
BB
Mukherjee
Partha S
PS
Sukla
Lala B
LB
Mishra
Barada K
BK
D042763:Acidithiobacillus / Q000737:chemistry* / Q000378:metabolism; D018441:Biofilms; D001704:Biopolymers / Q000737:chemistry / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D007194:India; D018625:Microscopy, Atomic Force / Q000379:methods*; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000378:metabolism; D012988:Soil Microbiology; D013059:Spectrum Analysis, Raman / Q000379:methods*; D014501:Uranium / Q000378:metabolism*
D001704:Biopolymers; D011135:Polysaccharides, Bacterial; D014501:Uranium