title: CD14-159 and -260 gene polymorphisms are associated with HBV-related cirrhotic injury in Chinese Han patients.
abstract: The present study aimed to investigate the association between TLR4 mutations (Asp299Gly and Thr399Ile) and CD14 polymorphisms (base pair -159 and -260) with HBV-related cirrhosis in Chinese Han patients. By use of a polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) analysis technique, we genotyped Toll-like receptor 4 (TLR4) Asp299Gly and Thr399Ile and CD14-159 and -260 polymorphisms in 110 HBV-related cirrhotic patients and 110 healthy controls from the Chinese Han population. We found significant differences in the genotypes and allele frequencies of CD14-159 (but not -260) between healthy controls and liver cirrhotic patients, and both the CD14-159 and -260 genotypes were significantly different among Child-Pugh grades in cirrhotic patients. No TLR4 Asp299Gly and Thr399Ile mutations were detected in any cirrhotic patients or healthy controls in the Chinese Han population. These findings indicated that the polymorphisms of CD14, but not TLR4 Asp299Gly and Thr399Ile mutations, may be an important genetic factor for HBV-related cirrhotic injury in the Chinese Han population.
authors.affiliation: Department of Internal Medicine, Shaoxing University School of Medicine, Shaoxing 312000, Zhejiang, PR China. ycg28@163.com
authors.forename: Chun-Gen
authors.initials: CG
authors.lastname: Yan
authors.forename: Guang-Yuan
authors.initials: GY
authors.lastname: Ren
authors.forename: Feng
authors.initials: F
authors.lastname: Wang
authors.forename: Dong-Fang
authors.initials: DF
authors.lastname: Zhu
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhuang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000595:Amino Acid Sequence; D002681:China; D005260:Female; D005787:Gene Frequency; D006509:Hepatitis B / Q000150:complications*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000235:genetics*; D008103:Liver Cirrhosis / Q000209:etiology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D051197:Toll-Like Receptor 4 / Q000235:genetics*
chemical_list: D018950:Lipopolysaccharide Receptors; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4