The efficiency of Viscum album ssp. album and Hypericum perforatum on human immune cells in vitro.
Viscum album L. ssp. album and Hypericum perforatum L. are used for the treatment of different diseases. In this study, the effects of these herbals on immune cells were assessed in vitro. The phagocytosis, candidacidal activity of neutrophils and adhesion function of epithelial cells were investigated. Also, the expression of the surface markers of lymphocytes was analyzed by flow cytometry. It was observed that V. album ssp. album increased phagocytic activity and candidacidal activity of neutrophils and decreased adhesion function of epithelial cells. We also observed that in human peripheral blood mononuclear cells stimulated by Viscum album L. ssp. album the levels of CD4(+)CD25(+) and CD8(+)CD25(+) T cells, CD69 expressions in the activated T lymphocytes and CD3(-)CD16(+)CD56(+) NK cells increased compared to the cells that were not stimulated by this herbal. Whereas CD4(+)CD25(+), CD8(+)CD25(+) T cells, CD 69 expression and CD3(-)CD16(+)CD56(+) Natural killer cells did not show any significant differences with the presence of Hypericum perforatum L. compared to the control group. Hypericum perforatum L. increased candidacidal activity of neutrophils and decreased adhesion function of epithelial cells. In the light of these findings, it is considered that these extracts may be used as an adjuvant treatment option for immune activation in immunosuppressed patients.
Fidan
Isil
I
Department of Medical Microbiology, Faculty of Medicine, Gazi University, Ankara, Turkey. isilfidan@yahoo.com
Ozkan
Semiha
S
Gurbuz
Ilhan
I
Yesilyurt
Emine
E
Erdal
Berna
B
Yolbakan
Sultan
S
Imir
Turgut
T
D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology*; D015703:Antigens, CD / Q000032:analysis; D001422:Bacterial Adhesion / Q000187:drug effects*; D002176:Candida albicans / Q000187:drug effects; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000187:drug effects; D004926:Escherichia coli / Q000187:drug effects; D005434:Flow Cytometry; D006801:Humans; D020902:Hypericum / Q000737:chemistry; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology; D009061:Mouth Mucosa / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D010587:Phagocytosis / Q000187:drug effects*; D035261:Plant Components, Aerial; D010936:Plant Extracts / Q000494:pharmacology; D028182:Viscum album / Q000737:chemistry
D000276:Adjuvants, Immunologic; D015703:Antigens, CD; D010936:Plant Extracts