title: Plant cell types: reporting and sampling with new technologies.
abstract: Plants have relatively few cell types, but their specialized functions and their interactions are essential for physiology, development, and defense. The contributions of individual cells have been distinguished by methods including in situ reporting, cell sampling, and cell separation, thus far mostly limited to measurement of single transcripts, proteins, or metabolites. Advances in transcriptomics, proteomics, metabolomics, and activity assays with small samples and in the modeling of these data into networks of expression, regulation, interaction, and metabolism make it possible to evaluate the roles of cell types at system levels. Recent analyses include cell types of developing roots, bundle sheath and mesophyll cells of C4-type leaves, xylem and phloem cells of vascular systems, and specialized regions of embryos and shoot apices.
authors.affiliation: Department of Molecular, Cellular and Developmental Biology, Yale University, P.O. Box 208104, New Haven, CT 06520-8104, USA. timothy.nelson@yale.edu
authors.forename: Timothy
authors.initials: T
authors.lastname: Nelson
authors.forename: Neeru
authors.initials: N
authors.lastname: Gandotra
authors.forename: S Lori
authors.initials: SL
authors.lastname: Tausta
mesh_terms: D002469:Cell Separation; D020869:Gene Expression Profiling / Q000379:methods; D016591:Histocytological Preparation Techniques; D059828:Plant Cells; D010944:Plants / Q000737:chemistry / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods