title: MITICS (MALDI Imaging Team Imaging Computing System): a new open source mass spectrometry imaging software.
abstract: MITICS is a new software developed for MALDI imaging. We tried to render this software compatible with all types of instruments. MITICS is divided in two parts: MITICS control for data acquisition and MITICS Image for data processing and images reconstruction. MITICS control is available for Applied BioSystems MALDI-TOF instruments and MITICS Image for both Applied BioSystems and Bruker Daltonics ones. MITICS Control provides an interface to the user for setting the acquisition parameters for the imaging sequence, namely set instruments acquisition parameters, create the raster of acquisition and control post-acquisition data processing, and provide this settings to the automatic acquisition software of the MALDI instrument. MITICS Image ensures image reconstruction, files are first converted to XML files before being loaded in a database. In MITICS image we have chosen to implement different data representations and calculations for image reconstruction. MITICS Image uses three different representations that have shown to ease extraction of information from the whole data set. It also offers image reconstruction base either on the maximum peak intensity or the peak area. Image reconstruction is possible for single ions but also by summing signals of different ions. MITICS was validated on biological cases.
authors.affiliation: University of Lille 1, CNRS FRE 2933, MALDI Imaging team, Laboratoire de Neuroimmunologie des Annélides, Villeneuve d'Ascq, France.
authors.forename: Olivia
authors.initials: O
authors.lastname: Jardin-Mathé
authors.forename: David
authors.initials: D
authors.lastname: Bonnel
authors.forename: Julien
authors.initials: J
authors.lastname: Franck
authors.forename: Maxence
authors.initials: M
authors.lastname: Wisztorski
authors.forename: Eduardo
authors.initials: E
authors.lastname: Macagno
authors.forename: Isabelle
authors.initials: I
authors.lastname: Fournier
authors.forename: Michel
authors.initials: M
authors.lastname: Salzet
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001331:Automation; D015415:Biomarkers / Q000737:chemistry; D001921:Brain / Q000378:metabolism; D019295:Computational Biology; D003196:Computer Graphics; D001330:Electronic Data Processing; D007091:Image Processing, Computer-Assisted / Q000379:methods; D008297:Male; D008969:Molecular Sequence Data; D010449:Peptide Mapping / Q000379:methods*; D051381:Rats; D017208:Rats, Wistar; D012984:Software; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*
chemical_list: D015415:Biomarkers