title: [Pathological experimental study of autologous skin transplant on retained denatured dermis].
abstract: OBJECTIVE
  To identify the pathological character of denatured dermis,and its turnover after autologous skin transplant.
  
  
  METHODS
  Deep partial thickness burn wounds whose diameter was 2.5 cm were produced on the back of Sprague-Dawley (SD) rats. After simple debriding,xenogenic skin was transplanted. Superficial tangential excision was performed on the burn wounds on 48 hours postburn with the preservation of denatured dermis. Split thickness autologous skin was grafted on the wounds immediately. Tissue samples of whole layer of the skin were harvested from the grafted sites at different time points after the skin grafting. Pathological observation on the denatured skin and the transplanted skin was carried out with HE and Massonos trichrome blue.
  
  
  RESULTS
  The superficial cells of the denatured dermis necrotized largely with few cells alive,collagen denatured,and many inflammatory cells infiltrating. Necrosis tissue and inflammatory cells could be found in the denatured skin in the early period after the skin transplant. There were infiltrated inflammatory cells in the transplanted skin 3 days after the skin transplant. On the 10th day,the necrotized tissue diminished markedly,and red cells were found in its upper stratum. On the 21st day, the morphology and structure of the transplanted skin were similar to those of the normal skin.
  
  
  CONCLUSIONS
  The retained denatured dermis has little effect on the survival of the transplanted skin. The necrosis components can be absorbed and replaced by the tissue alive after the autologous skin is transplanted.
authors.affiliation: Department of Burn and Plastics Surgery, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Peng-Ju
authors.initials: PJ
authors.lastname: Fan
authors.forename: Xiao-Yuan
authors.initials: XY
authors.lastname: Huang
authors.forename: Xing-Hua
authors.initials: XH
authors.lastname: Yang
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology* / Q000601:surgery*; D020405:Dermis / Q000637:transplantation; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000473:pathology*; D016038:Skin Transplantation / Q000379:methods*; D014182:Transplantation, Autologous