title: Streptococcus iniae M-like protein contributes to virulence in fish and is a target for live attenuated vaccine development.
abstract: BACKGROUND
  Streptococcus iniae is a significant pathogen in finfish aquaculture, though knowledge of virulence determinants is lacking. Through pyrosequencing of the S. iniae genome we have identified two gene homologues to classical surface-anchored streptococcal virulence factors: M-like protein (simA) and C5a peptidase (scpI).
  
  
  RESULTS
  S. iniae possesses a Mga-like locus containing simA and a divergently transcribed putative mga-like regulatory gene, mgx. In contrast to the Mga locus of group A Streptococcus (GAS, S. pyogenes), scpI is located distally in the chromosome. Comparative sequence analysis of the Mgx locus revealed only one significant variant, a strain with an insertion frameshift mutation in simA and a deletion mutation in a region downstream of mgx, generating an ORF which may encode a second putative mga-like gene, mgx2. Allelic exchange mutagenesis of simA and scpI was employed to investigate the potential role of these genes in S. iniae virulence. Our hybrid striped bass (HSB) and zebrafish models of infection revealed that M-like protein contributes significantly to S. iniae pathogenesis whereas C5a peptidase-like protein does not. Further, in vitro cell-based analyses indicate that SiMA, like other M family proteins, contributes to cellular adherence and invasion and provides resistance to phagocytic killing. Attenuation in our virulence models was also observed in the S. iniae isolate possessing a natural simA mutation. Vaccination of HSB with the Delta simA mutant provided 100% protection against subsequent challenge with a lethal dose of wild-type (WT) S. iniae after 1,400 degree days, and shows promise as a target for live attenuated vaccine development.
  
  
  CONCLUSIONS
  Analysis of M-like protein and C5a peptidase through allelic replacement revealed that M-like protein plays a significant role in S. iniae virulence, and the Mga-like locus, which may regulate expression of this gene, has an unusual arrangement. The M-like protein mutant created in this research holds promise as live-attenuated vaccine.
authors.affiliation: Department of Pediatrics, University of California San Diego, La Jolla, California, United States of America.
authors.forename: Jeffrey B
authors.initials: JB
authors.lastname: Locke
authors.forename: Ramy K
authors.initials: RK
authors.lastname: Aziz
authors.forename: Mike R
authors.initials: MR
authors.lastname: Vicknair
authors.forename: Victor
authors.initials: V
authors.lastname: Nizet
authors.forename: John T
authors.initials: JT
authors.lastname: Buchanan
mesh_terms: D018829:Adhesins, Bacterial / Q000378:metabolism; D000483:Alleles; D000595:Amino Acid Sequence; D000818:Animals; D019295:Computational Biology / Q000379:methods; D010450:Endopeptidases / Q000378:metabolism; D005399:Fishes / Q000378:metabolism*; D005786:Gene Expression Regulation; D008957:Models, Genetic; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D017386:Sequence Homology, Amino Acid; D013291:Streptococcus / Q000378:metabolism*; D014612:Vaccines / Q000737:chemistry; D014774:Virulence; D037521:Virulence Factors / Q000378:metabolism / Q000502:physiology*
chemical_list: D018829:Adhesins, Bacterial; D014612:Vaccines; D037521:Virulence Factors; D010450:Endopeptidases; C053088:C5a peptidase