[Oligomeric organization of recombinant human neurotrophins expressed in Escherichia coli cells].
Genes of human neurotrophins NGF, BDNF, NT-3 were cloned, and the corresponding proteins and their fragments were expressed in Escherichia coli BL-21 (DE3lambda) cells. Their intracellular localization was determined. The conditions for isolation and purification of the target recombinant proteins and for folding of BDNF and NT-3 precursors were selected. The recombinant proprecursors of human neurotrophines have been shown to possess complex oligomeric structure.
Safina
D R
DR
Rafieva
L M
LM
Koval'
A V
AV
Shkurina
E E
EE
Dmitrieva
V G
VG
Raevskaia
N M
NM
Gasanov
E V
EV
Demidiuk
I V
IV
Kostrov
S V
SV
D001704:Biopolymers; D019208:Brain-Derived Neurotrophic Factor / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D020932:Nerve Growth Factor / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D020933:Neurotrophin 3 / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D010446:Peptide Fragments / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D011489:Protein Denaturation; D017510:Protein Folding; D011498:Protein Precursors / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics
D001704:Biopolymers; D019208:Brain-Derived Neurotrophic Factor; D020933:Neurotrophin 3; D010446:Peptide Fragments; D011498:Protein Precursors; D011994:Recombinant Proteins; D020932:Nerve Growth Factor