G-quadruplex-based DNAzyme for facile colorimetric detection of thrombin.
Thrombin-binding aptamer is found to bind hemin to form a catalytic complex whose activity is significantly promoted by the addition of thrombin, which enables the colorimetric detection of thrombin with high specificity and sensitivity in a facile way.
Li
Tao
T
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, China.
Wang
Erkang
E
Dong
Shaojun
S
D052157:Aptamers, Nucleotide / Q000737:chemistry; D001483:Base Sequence; D003124:Colorimetry / Q000379:methods*; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism*; D054856:G-Quadruplexes; D006427:Hemin / Q000737:chemistry; D011485:Protein Binding; D013917:Thrombin / Q000032:analysis* / Q000737:chemistry
D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; D006427:Hemin; D013917:Thrombin