[Study on isolation of Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique].
OBJECTIVE
To isolate and purify Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique.


METHODS
Chlorogenic acid transferring ratio was investigated by temperature, pressure, time, cut-off molecular mass.


RESULTS
The optimal conditions for the ultrafiltration technique were finally determined. It was found that, for the membrane with a cut-off molecular mass of 20000, when the temperature, pressure, time were respectively 40 degrees C, 0.36 MPa, 20 minutes and adding 24L water, yield of Chlorogenic acid was 99.38%.


CONCLUSIONS
High Chlorogenic acid transfering ratio can be gotten by ultrafiltration technique, which is helpful to the further isolation and purification of Chlorogenic acid in the subsequent process.
Yang
Zu-Jin
ZJ
National Engineering Research Center for Modernization of Extraction and Separation Process of TCM, Guangzhou 510240, China. yangzujin@126.com
Jiang
Yan-Bin
YB
Ge
Fa-Huan
FH
Yuan
Yu-Jie
YJ
Yu
Shao-Chong
SC
D002726:Chlorogenic Acid / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D031283:Eucommiaceae / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D014462:Ultrafiltration / Q000379:methods
D004365:Drugs, Chinese Herbal; D002726:Chlorogenic Acid