title: Genus-specific PCR for molecular identification of novel isolates of the genus Nesterenkonia.
abstract: OBJECTIVE
  For rapid identification of Nesterenkonia species, one set of genus-specific primers was designed and synthesized for polymerase chain reaction based on the 16S rRNA gene sequences.
  
  
  CONCLUSIONS
  The genus specificity of these primers was validated with reference strains as well as with wild-type isolates. Partial sequencing results of 16S rRNA gene of the wild-type isolates confirmed that they are members of the genus Nesterenkonia.
authors.affiliation: Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming 650091, China.
authors.forename: Xiaoyang
authors.initials: X
authors.lastname: Zhi
authors.forename: Lingling
authors.initials: L
authors.lastname: Yang
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.forename: Shukun
authors.initials: S
authors.lastname: Tang
authors.forename: Kai
authors.initials: K
authors.lastname: Lou
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
mesh_terms: D044822:Biodiversity; D017931:DNA Primers / Q000235:genetics; D008835:Micrococcaceae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D013045:Species Specificity
chemical_list: D017931:DNA Primers; D012336:RNA, Ribosomal, 16S