Cadherin-7 function in zebrafish development.
Cadherin cell adhesion molecules play crucial roles in vertebrate development. Most studies have focused on examining the functions of classical type I cadherins (e.g., cadherin-2) in the development of vertebrates. Little information is available concerning the function of classical type II cadherins (e.g., cadherin-7) in vertebrate development. We have previously shown that cadherin-7 mRNA exhibits a dynamic expression pattern in the central nervous system and notochord in embryonic zebrafish. To gain insight into the role of cadherin-7 in the formation of these structures, we analyzed their formation in zebrafish embryos injected with cadherin-7-specific antisense morpholino oligonucleotides (MO). Notochord development was severely disrupted in MO-injected embryos, whereas gross defects in the development of the central nervous system were not detected in MO-injected embryos. Our results thus demonstrate that cadherin-7 plays an important role in the normal development of the zebrafish notochord.
Liu
Qin
Q
Department of Biology, University of Akron, Akron, OH 44325, USA. qliu@uakron.edu
Marrs
James A
JA
Londraville
Richard L
RL
Wilson
Amy L
AL
D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D015820:Cadherins / Q000235:genetics / Q000502:physiology*; D002490:Central Nervous System / Q000187:drug effects / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000187:drug effects / Q000502:physiology; D018507:Gene Expression Regulation, Developmental; D009672:Notochord / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D016376:Oligonucleotides, Antisense / Q000235:genetics; D015027:Zebrafish / Q000196:embryology* / Q000502:physiology; D029961:Zebrafish Proteins / Q000235:genetics / Q000502:physiology*
D015415:Biomarkers; D015820:Cadherins; D016376:Oligonucleotides, Antisense; D029961:Zebrafish Proteins; C534999:cadherin-7, zebrafish