Quantitative proteomics as a new piece of the systems biology puzzle.
The definition of the role of each gene product in its cellular context is of outstanding importance in the post-genomics era. Recent technological innovations have driven research in proteomics from single protein characterization to global approaches, aiming to achieve a comprehensive qualitative and quantitative description of complex molecular mechanisms. In this review, we discuss the methodology of quantitative proteomics as it applies to the analysis of complex biological model systems. A special attention will be given to model systems that are suitable for functional genomic studies, where the potential of quantitative proteomics can be effectively demonstrated.
Bachi
Angela
A
DIBIT, San Raffaele Scientific Institute, Milan, Italy.
Bonaldi
Tiziana
T
D000818:Animals; D017360:Arabidopsis / Q000378:metabolism; D017173:Caenorhabditis elegans; D019295:Computational Biology; D004331:Drosophila melanogaster; D004867:Equipment Design; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods; D051379:Mice; D008954:Models, Biological; D040901:Proteomics / Q000295:instrumentation* / Q000379:methods*; D051381:Rats; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D012984:Software; D049490:Systems Biology / Q000379:methods*