Hyperbaric oxygen therapy aggravates liver reperfusion injury in rats.
OBJECTIVE
To evaluate the effects of hyperbaric oxygen (HO) therapy in the protection against liver ischemia/reperfusion injury.


METHODS
Thirty-two male Wistar rats were divided into four groups of eight animals each: group A - laparotomy and liver manipulation, group B - liver ischemia and reperfusion, group C - HO pretreatment for 60 min followed by liver ischemia and reperfusion, and group D - pretreatment with ambient air at 2.5 absolute atmospheres for 60 min followed by liver ischemia and reperfusion. Plasma was assayed for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Intra-arterial blood pressure was monitored continuously. Myeloperoxidase activity in the liver and lung was assessed 30 min after reperfusion.


RESULTS
Plasma AST, ALT and LDH increased after reperfusion in all animals. Plasma ALT values and myeloperoxidase activity in the liver parenchyma were higher in HO-pretreated animals than in groups A, B and D. HO had a negative hemodynamic effect during liver reperfusion.


CONCLUSIONS
Liver preconditioning with hyperbaric oxygen therapy aggravated liver ischemia/reperfusion injury in rats as demonstrated by plasma ALT and liver myeloperoxidase activity.
Lima
Cristiano Xavier
CX
Department of Physiology, School of Science, Federal University of Minas Gerais, Brazil. cxlima@globo.com
Sanches
Marcelo Dias
MD
Rezende Neto
João Baptista de
JB
Silva
Roberto Carlos de Oliveira e
RC
Teixeira
Mauro Martins
MM
Souza
Danielle da Glória de
Dda G
Santos
Guilherme de Castro
Gde C
Melo
José Renan da Cunha
JR
D000410:Alanine Transaminase / Q000097:blood*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001794:Blood Pressure / Q000502:physiology; D001795:Blood Pressure Determination; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D019194:Ischemic Preconditioning / Q000009:adverse effects*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000098:blood supply*; D008107:Liver Diseases / Q000209:etiology* / Q000517:prevention & control; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology* / Q000517:prevention & control
D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase