Establishment of rat embryonic stem cells and making of chimera rats.
The rat is a reference animal model for physiological studies and for the analysis of multigenic human diseases such as hypertension, diabetes, neurological disorders, and cancer. The rats have long been used in extensive chemical carcinogenesis studies. Thus, the rat embryonic stem (rES) cell is an important resource for the study of disease models. Attempts to derive ES cells from various mammals, including the rat, have not succeeded. Here we have established two independent rES cells from Wister rat blastocysts that have undifferentiated characters such as Nanog and Oct3/4 genes expression and they have stage-specific embryonic antigen (SSEA) -1, -3, -4, and TRA-1-81 expression. The cells were successfully cultured in an undifferentiated state and can be possible over 18 passages with maintaining more than 40% of normal karyotype. Their pluripotent potential was confirmed by the differentiation into derivatives of the endoderm, mesoderm, and ectoderm. Most importantly, the rES cells are capable of producing chimera rats. Therefore, we established pluripotent rES cell lines that are widely used to produce genetically modified experimental rats for study of human diseases.
Ueda
Shinobu
S
Section for Studies on Metastasis, National Cancer Center Research Institute, Tokyo, Japan.
Kawamata
Masaki
M
Teratani
Takumi
T
Shimizu
Taku
T
Tamai
Yoshitaka
Y
Ogawa
Hiromasa
H
Hayashi
Katsuyuki
K
Tsuda
Hiroyuki
H
Ochiya
Takahiro
T
D000818:Animals; D001755:Blastocyst / Q000166:cytology / Q000378:metabolism; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D002678:Chimera; D004195:Disease Models, Animal; D053595:Embryonic Stem Cells / Q000166:cytology*; D005260:Female; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007621:Karyotyping; D008297:Male; D008954:Models, Biological; D051381:Rats; D017208:Rats, Wistar
D049452:Green Fluorescent Proteins