Stimulation of human omental adipose tissue lipolysis by growth hormone plus dexamethasone.
Growth hormone [GH] administration results in a reduction in adiposity of humans that is attributed to stimulation of lipolysis. We examined the effect of direct addition of human GH, in both the absence and presence of dexamethasone [Dex], as well as that of interferon beta on lipolysis by omental adipose tissue explants from obese women incubated for 48h in primary culture. There was a significant stimulation of lipolysis by GH in the presence of Dex but not by Dex or GH alone. There was also a significant further stimulation by GH in the presence of Dex of hormone-sensitive lipase, perilipin, lipoprotein lipase and beta1 adrenergic receptor mRNA. We conclude that the direct lipolytic effect of GH is accompanied by an increase in HSL mRNA in the presence of DEX, but GH also increased the mRNAs for other proteins that could explain all or part of its lipolytic action.
Fain
John N
JN
Department of Molecular Sciences, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA. jfain@utmen.edu
Cheema
Paramjeet
P
Tichansky
David S
DS
Madan
Atul K
AK
D002352:Carrier Proteins; D003907:Dexamethasone / Q000494:pharmacology*; D005260:Female; D005938:Glucocorticoids / Q000494:pharmacology*; D019382:Human Growth Hormone / Q000378:metabolism*; D006801:Humans; D016899:Interferon-beta / Q000378:metabolism; D050152:Intra-Abdominal Fat / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D008049:Lipase / Q000235:genetics / Q000378:metabolism; D008066:Lipolysis / Q000187:drug effects*; D009767:Obesity, Morbid / Q000201:enzymology / Q000378:metabolism*; D009852:Omentum; D000070778:Perilipin-1; D010750:Phosphoproteins / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D018342:Receptors, Adrenergic, beta-1 / Q000235:genetics / Q000378:metabolism; D002787:Sterol Esterase / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D046509:Tissue Culture Techniques; D000217:Acyltransferases
D002352:Carrier Proteins; D003907:Dexamethasone; D005938:Glucocorticoids; D019382:Human Growth Hormone; D016899:Interferon-beta; D008049:Lipase; D000070778:Perilipin-1; D010750:Phosphoproteins; D012333:RNA, Messenger; D018342:Receptors, Adrenergic, beta-1; D002787:Sterol Esterase; C494794:PNPLA2 protein, human; D000217:Acyltransferases