title: Inactivation of Listeria monocytogenes in brine and saline by alternating high-voltage pulsed current.
abstract: The inactivating efficiency of alternating high-voltage pulsed (AHVP) current was investigated in brine (20 w/v% NaCl) and saline (0.9 w/v% NaCl) inoculated with 1x 10(7) cells/ml of Listeria monocytogenes. AHVP current at 12 V with 1 pulse completely inactivated L. monocytogenes in brine within 3 ms, while the bacteria in saline were fully inactivated by 10-pulsed electric treatment at 12 V within the same time. Electron microscopic observation demonstrated substantial structural damage of electrically treated L. monocytogenes in brine. These results suggest that AHVP treatment would be effective for the rapid and complete inactivation of L. monocytogenes in brine or saline solution.
authors.affiliation: Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea.
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Lee
authors.forename: Dong-Wook
authors.initials: DW
authors.lastname: Han
authors.forename: Yeon I
authors.initials: YI
authors.lastname: Woo
authors.forename: Masakazu
authors.initials: M
authors.lastname: Uzawa
authors.forename: Jong-Chul
authors.initials: JC
authors.lastname: Park
mesh_terms: D004560:Electricity; D005516:Food Microbiology; D005519:Food Preservation / Q000379:methods*; D008089:Listeria monocytogenes / Q000187:drug effects / Q000254:growth & development*; D050296:Microbial Viability / Q000187:drug effects; D012965:Sodium Chloride / Q000494:pharmacology*
chemical_list: D012965:Sodium Chloride