First molecular method for discriminating between Candida africana, Candida albicans, and Candida dubliniensis by using hwp1 gene.
The authors report here a polymerase chain reaction-based assay using a single primer pair (CR-f/CR-r), allowing discrimination between Candida africana and Candida albicans by using the hwp1 gene. The method also identifies Candida dubliniensis because it produces 3 different DNA fragments: approximately 700 bp for C. africana, 941 bp for C. albicans, and 569 for C. dubliniensis.
Romeo
Orazio
O
Department of Microbiological, Genetic and Molecular Sciences University of Messina, Salita Sperone, 31 98166 Messina, Italy.
Criseo
Giuseppe
G
D002175:Candida / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002176:Candida albicans / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002180:Candidiasis, Oral / Q000382:microbiology; D002181:Candidiasis, Vulvovaginal / Q000382:microbiology; D005260:Female; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D008562:Membrane Glycoproteins / Q000235:genetics*; D008969:Molecular Sequence Data; D016533:Mycological Typing Techniques; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA
D005656:Fungal Proteins; C118102:HWP1 protein, Candida albicans; D008562:Membrane Glycoproteins