Differential expression of two interferon-gamma genes in common carp (Cyprinus carpio L.).
Two interferon gamma (IFN-gamma) genes are expressed in immune cells of teleost fish and are potentially implicated in B- and T-lymphocyte responses. IFN-gamma-2 shows structural and functional characteristics to other vertebrate IFN-gamma genes and is associated with T-lymphocyte function. Expression profiling shows IFN-gamma-2 upregulation in T-lymphocytes after phytohemagglutinin (PHA) stimulation in vitro. Unexpectedly, we found IFN-gamma-1, which is structurally different from IFN-gamma-2, to be expressed in lipopolysacharide (LPS)-stimulated IgM+ (B- lymphocyte enriched) fractions. Expression of T-box transcription factor T-bet, but not of GATA-binding protein 3 (GATA3), correlated with expression of both IFN-gamma genes. In-vivo parasite infection, but as predicted not zymosan-induced inflammation, resulted in concomitant upregulation of T-bet and IFN-gamma-2. This corroborates a genuine T-lymphocyte associated role for IFN-gamma-2.
Stolte
Ellen H
EH
Cell Biology and Immunology Group, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands.
Savelkoul
Huub F J
HF
Wiegertjes
Geert
G
Flik
Gert
G
Lidy Verburg-van Kemenade
B M
BM
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002347:Carps / Q000235:genetics* / Q000276:immunology*; D002417:Cattle; D002478:Cells, Cultured; D004285:Dogs; D005786:Gene Expression Regulation / Q000502:physiology*; D006801:Humans; D007371:Interferon-gamma / Q000096:biosynthesis* / Q000235:genetics* / Q000302:isolation & purification; D051379:Mice; D008969:Molecular Sequence Data; D020033:Protein Isoforms / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D011817:Rabbits; D051381:Rats; D016415:Sequence Alignment
D020033:Protein Isoforms; D007371:Interferon-gamma