DjlA, a membrane-anchored DnaJ-like protein, is required for cytotoxicity of clam pathogen Vibrio tapetis to hemocytes.
DjlA is an inner membrane cochaperone belonging to the DnaJ family, which has been shown to be involved in Legionella sp. pathogenesis. In this study, we explored the role of this protein in the physiology and virulence of Vibrio tapetis, the etiological agent of brown ring disease (BRD) in Manila clam (Ruditapes philippinarum). Analysis of the djlA locus in V. tapetis revealed a putative organization in an operon with a downstream gene that we designated duf924(Vt), which encodes a conserved protein with an unknown function and has homologues in bacteria and eukaryotes. djlA mutants displayed a reduced growth rate and showed an important loss of cytotoxic activity against R. philippinarum hemocytes in vitro, which could be restored by extrachromosomal expression of wild-type djlA(Vt) but not duf924(Vt). These results are in keeping with the potential importance of DjlA for bacterial pathogenicity and open new perspectives for understanding the mechanism of action of this protein in the novel V. tapetis-R. philippinarum interaction model.
Lakhal
Fatma
F
Institut de Génétique et Microbiologie, Bâtiment 400, Université Paris-Sud, Orsay 91405 Cedex, France.
Bury-Moné
Stéphanie
S
Nomane
Yanoura
Y
Le Goïc
Nelly
N
Paillard
Christine
C
Jacq
Annick
A
D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D049872:Bivalvia / Q000382:microbiology*; D003227:Conjugation, Genetic; D004269:DNA, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics; D005798:Genes, Bacterial; D050956:HSP40 Heat-Shock Proteins / Q000235:genetics*; D006434:Hemocytes / Q000382:microbiology*; D008969:Molecular Sequence Data; D009876:Operon; D010641:Phenotype; D010957:Plasmids; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D014733:Vibrio / Q000235:genetics* / Q000472:pathogenicity; D014735:Vibrio Infections / Q000382:microbiology*; D014774:Virulence
D001426:Bacterial Proteins; D004269:DNA, Bacterial; D050956:HSP40 Heat-Shock Proteins