Assessment of fungicide systemicity in wheat using LC-MS/MS.
BACKGROUND
Systemicity is an important attribute of fungicides that is difficult to measure in early-stage screening without labeling the compound with a radioisotope. A method of measuring translocation that does not require potent fungicidal activity or a radiolabel would guide identification of compounds with desirable attributes.


RESULTS
The authors developed an analytical technique that mimics field application, using LC-MS/MS to screen compounds for translocation in wheat leaves. The method sorted commercial and experimental fungicides appropriately into systemic and non-systemic categories. A model using LC-MS/MS data was equivalent to a lipophilicity model and superior to a water solubility model at predicting compound systemicity.


CONCLUSIONS
Early-stage compounds can be screened for systemicity on whole plants using LC-MS/MS.
Klittich
Carla
C
Dow AgroSciences LLC, Discovery Research, Indianapolis, IN 46268, USA. cklittich@dow.com
Green
Fredrick R
FR
Ruiz
James M
JM
Weglarz
Ted
T
Blakeslee
Beth A
BA
D002853:Chromatography, Liquid; D005659:Fungicides, Industrial / Q000737:chemistry / Q000493:pharmacokinetics*; D018515:Plant Leaves / Q000378:metabolism*; D015203:Reproducibility of Results; D018401:Sample Size; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D014908:Triticum / Q000378:metabolism*
D005659:Fungicides, Industrial