title: Toward the discovery of potent inhibitors of botulinum neurotoxin A: development of a robust LC MS based assay operational from low to subnanomolar enzyme concentrations.
abstract: The development of a sensitive, yet reliable assay for the analysis of botulinum neurotoxin A (BoNT/A) inhibitors is described; using this assay a new protease inhibitor was characterized and found to be one of the most potent inhibitors reported to date.
authors.affiliation: Department of Chemistry, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Katerina
authors.initials: K
authors.lastname: Capková
authors.forename: Mark S
authors.initials: MS
authors.lastname: Hixon
authors.forename: Laura A
authors.initials: LA
authors.lastname: McAllister
authors.forename: Kim D
authors.initials: KD
authors.lastname: Janda
mesh_terms: D000218:Adamantane / Q000031:analogs & derivatives / Q000737:chemistry* / Q000494:pharmacology*; D001667:Binding, Competitive; D019274:Botulinum Toxins, Type A / Q000037:antagonists & inhibitors* / Q000737:chemistry*; D002384:Catalysis; D002851:Chromatography, High Pressure Liquid; D004305:Dose-Response Relationship, Drug; D004353:Drug Evaluation, Preclinical / Q000379:methods*; D005453:Fluorescence; D013058:Mass Spectrometry; D012680:Sensitivity and Specificity
chemical_list: D019274:Botulinum Toxins, Type A; D000218:Adamantane