title: An operative approach to correct CD spectra distortions due to absorption flattening.
abstract: We discuss the influence of absorption flattening (AF) on CD spectra, aiming at finding a simple way to compensate the induced distortions. A simple algebraic formalism is proposed based on an unconventional measuring approach, which makes use of a commercial unit. Validation of the proposed compensation method for AF is conducted not only on the already studied CD Vis spectral region of a Co chiral complex with the use of an AF emulator, but also on the far UV CD spectra of poly-L-glutamic acid suspensions with different turbidity levels. Comparison with some correction factors proposed in the literature is presented.
authors.affiliation: JASCO Corporation, Hachioji-Shi, Tokyo, Japan. ettore.castiglioni@jasco-europe.com
authors.forename: Ettore
authors.initials: E
authors.lastname: Castiglioni
authors.forename: France
authors.initials: F
authors.lastname: Lebon
authors.forename: Giovanna
authors.initials: G
authors.lastname: Longhi
authors.forename: Roberto
authors.initials: R
authors.lastname: Gangemi
authors.forename: Sergio
authors.initials: S
authors.lastname: Abbate
mesh_terms: D002942:Circular Dichroism / Q000379:methods*; D003035:Cobalt / Q000737:chemistry; D011099:Polyglutamic Acid / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
chemical_list: D011099:Polyglutamic Acid; D003035:Cobalt