title: High-performance liquid chromatography and LC-ESI-MS method for the identification and quantification of two biologically active isomeric coumarinolignoids cleomiscosin A and cleomiscosin B in different extracts of Cleome viscosa.
abstract: A rapid, sensitive and simple reverse-phase high-performance liquid chromatographic-electrospray ionization-mass spectrometry method for simultaneous determination of cleomiscosin A and cleomiscosin B has been developed and validated. The isomeric coumarinolignoids cleomiscosin A (1) and cleomiscosin B (2) were separated on a Waters symmetry C(18) column with a solvent system composed of acetonitrile-methanol (1:2) and acetic acid-water (0.5:99.5) in a gradient elution mode. The absorption at 326 nm was chosen as the measuring wavelength in which resolution and baseline separation of compounds 1 and 2 could be obtained. The identity of the two isomeric compounds 1 and 2 in the samples were determined on a triple quadrupole mass spectrometer with ESI interface operating in the positive mode. Calibration curves were linear (r(2) > 0.993) over the concentration range 20-200 microg/mL for cleomiscosin A and 10-200 microg/mL for cleomiscosin B with acceptable accuracy and precision, respectively. The intra-day and inter-day precision were 1.13 and 0.82% for cleomiscosin A and 1.78 and 1.28% for cleomiscosin B, respectively. The validated method was successfully applied for the analysis of the above two compounds in different extracts of Cleome viscosa.
authors.affiliation: Central Institute of Medicinal and Aromatic Plants, (CSIR), PO CIMAP, Lucknow 226015, India. chattsk@yahoo.com
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Chattopadhyay
authors.forename: Satyanshu
authors.initials: S
authors.lastname: Kumar
authors.forename: Sarita
authors.initials: S
authors.lastname: Tripathi
authors.forename: Ranjeet
authors.initials: R
authors.lastname: Kaur
authors.forename: Sudeep
authors.initials: S
authors.lastname: Tandon
authors.forename: Shailendra
authors.initials: S
authors.lastname: Rane
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D031485:Cleome / Q000737:chemistry*; D003374:Coumarins / Q000032:analysis* / Q000737:chemistry; D007536:Isomerism; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D003374:Coumarins; D010936:Plant Extracts; C041934:cleomiscosin A