title: Aortic smooth muscle cells migration and the role of metalloproteinases and hyaluronan.
abstract: Human aortic smooth muscle cells (AoSMCs) change their extracellular matrix composition during aging, with direct effects on cellular events and cell migration. For example, active matrix metalloproteinase-2 (MMP-2) is synthesized only by young AoSMCs, whereas aged cells produce only the inactive zymogen form. The pro-MMP-2 activation in young cells depends on an increase in membrane type 1 matrix metalloproteinase content. Furthermore, transcripts coding for tissue inhibitor of metalloproteinases (TIMPs) were upregulated in aged cells, and the increase of TIMPs also could prevent pro-MMP-2 activation. As consequence of these situations, young AoSMCs possess a higher migratory capability than aged cells on gelatin support. These data are confirmed by adding TIMP-1 and TIMP-2 to young cells which reproduces aged AoSMCs migratory behavior. The opposite effect was obtained in young cells silencing MMP-2 and TIMP-2.
authors.affiliation: Department of Experimental Biomedical and Clinical Sciences, University of Insubria, Varese, Italy.
authors.forename: Davide
authors.initials: D
authors.lastname: Vigetti
authors.forename: Paola
authors.initials: P
authors.lastname: Moretto
authors.forename: Manuela
authors.initials: M
authors.lastname: Viola
authors.forename: Anna
authors.initials: A
authors.lastname: Genasetti
authors.forename: Manuela
authors.initials: M
authors.lastname: Rizzi
authors.forename: Evgenia
authors.initials: E
authors.lastname: Karousou
authors.forename: Moira
authors.initials: M
authors.lastname: Clerici
authors.forename: Barbara
authors.initials: B
authors.lastname: Bartolini
authors.forename: Francesco
authors.initials: F
authors.lastname: Pallotti
authors.forename: Giancarlo
authors.initials: G
authors.lastname: De Luca
authors.forename: Alberto
authors.initials: A
authors.lastname: Passi
mesh_terms: D000818:Animals; D001011:Aorta; D002465:Cell Movement; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000378:metabolism*; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism*; D009131:Muscle, Smooth, Vascular / Q000166:cytology* / Q000502:physiology; D032389:Myocytes, Smooth Muscle / Q000201:enzymology / Q000502:physiology*; D019714:Tissue Inhibitor of Metalloproteinases / Q000378:metabolism*
chemical_list: D018960:Hyaluronan Receptors; D019714:Tissue Inhibitor of Metalloproteinases; D006820:Hyaluronic Acid; D020778:Matrix Metalloproteinase 2