Genetic diversity in grapevine germplasm resources in the Coruh valley revealed by RAPD markers.
Random amplified polymorphic DNA analysis was carried out in 35 autochthonous table grapevine cultivars grown in Coruh valley. Fifty-five oligonucleotide primers were screened on cultivars, and among them, 12 primers showed clear polymorphic patterns. PCR amplification with 12 primers generated a total of 157 polymorphic bands. There was genetic variation among the cultivars with values of genetic diversity ranging from 0.19 to 0.72 using the Jaccard coefficient. UPGMA analysis of the distance matrix resulted in a dendrogram with two main clusters. The first cluster included 28 cultivars and the second 7 cultivars. The greatest genetic similarity was observed between cultivars Gah and Kolik, while the greatest dissimilarity was observed between cultivars Gah and Siyah Kus Uzumu. The dendrogram revealed that the cultivars present in Coruh valley can be distinguished to a relatively high degree.
Ercisli
Sezai
S
Department of Horticulture, Ataturk University, Erzurum, 25240, Turkey. sercisli@hotmail.com
Orhan
Emine
E
Hizarci
Yasemin
Y
Yildirim
Nalan
N
Agar
Guleray
G
D015415:Biomarkers; D017931:DNA Primers / Q000737:chemistry / Q000235:genetics; D018744:DNA, Plant / Q000235:genetics*; D017343:Genes, Plant; D014644:Genetic Variation; D005838:Genotype; D010641:Phenotype; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D027843:Vitis / Q000235:genetics*
D015415:Biomarkers; D017931:DNA Primers; D018744:DNA, Plant