Antitumor properties of a sulfated polysaccharide from the red seaweed Champia feldmannii (Diaz-Pifferer).
In recent years, much attention has been focused on polysaccharides isolated from natural sources. The aim of this study was to investigate the in vitro and in vivo antitumor properties of a sulfated polysaccharide isolated from the seaweed C. feldmannii (Cf-PLS). Hematological, biochemical and histopathological analyses were performed in order to evaluate the toxicological aspects related to Cf-PLS treatment. Its effects on the immunological system were also investigated. The Cf-PLS did not show any significant in vitro cytotoxicity at the experimental exposure levels that were used, but showed in vivo antitumor effect. The inhibition rates of sarcoma 180 tumor development were 48.62 and 48.16% at the doses of 10 and 25 mg kg(-1), respectively. In addition, Cf-PLS was also able to increase the response elicited by 5-fluorouracil (5-FU) from 48.66 to 68.32%. The histopathological analysis of liver and kidney showed that both organs were moderately affected by Cf-PLS-treatment. Neither enzymatic activity of alanine aminotransferase nor urea or creatinine levels were significantly altered. In hematological analysis, leucopeny was observed after 5-FU treatment, but this effect was prevented when the treatment was associated with the Cf-PLS. It was also demonstrated that Cf-PLS acts as an immunomodulatory agent, raising the production of specific antibodies, and increasing the production of OVA-specific antibodies. It also induced a discreet hyperplasia of lymphoid folicules of the white pulp in the spleen of treated mice. In conclusion, Cf-PLS has some interesting anticancer activity that could be associated with its immunostimulating properties.
Lins
Kézia O A L
KO
Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceará, 60430-270 Fortaleza, Ceará, Brazil.
Bezerra
Daniel P
DP
Alves
Ana Paula N N
AP
Alencar
Nylane M N
NM
Lima
Michael W
MW
Torres
Valeska M
VM
Farias
Wladimir R L
WR
Pessoa
Cláudia
C
de Moraes
Manoel O
MO
Costa-Lotufo
Letícia V
LV
D000818:Animals; D000917:Antibody Formation / Q000187:drug effects; D000970:Antineoplastic Agents / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor / Q000187:drug effects* / Q000473:pathology; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D004357:Drug Synergism; D004359:Drug Therapy, Combination; D005260:Female; D005472:Fluorouracil / Q000494:pharmacology; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology / Q000633:toxicity; D051379:Mice; D010936:Plant Extracts / Q000494:pharmacology / Q000633:toxicity; D011134:Polysaccharides / Q000494:pharmacology* / Q000633:toxicity; D000461:Rhodophyta / Q000737:chemistry*; D012510:Sarcoma 180 / Q000188:drug therapy* / Q000473:pathology; D012624:Seaweed; D013431:Sulfates / Q000494:pharmacology / Q000633:toxicity; D018675:Toxicity Tests
D000970:Antineoplastic Agents; D007155:Immunologic Factors; D010936:Plant Extracts; D011134:Polysaccharides; D013431:Sulfates; D005472:Fluorouracil