title: Interaction of avian reovirus with chicken lymphoblastoid cell lines.
abstract: Four chicken lymphoblastoid cell lines were inoculated with avian reovirus strain S1133 and two local isolates, 965 and 615. Of the inoculated cell lines, TLT, a B-cell line, was productively infected with the three viruses as demonstrated by immunofluorescence assay (IFA) and radioimmunoprecipitation assay. A comparative growth curve analysis of the three avian reoviruses was done at 37 degrees and 41 degrees C. Isolate 965 replicated to a higher titre at both temperatures while the replication of S1133 and 615 was found to be inhibited at 41 degrees C. IFA revealed that among the transformed T lymphoblastoid cells used in this study, only MDCC-RP1 was permissive to virus infection with isolate 965, and at 41 degrees C, but not 37 degrees C.
authors.affiliation: Departement de pathologie et microbiologie, Faculte de medecine veterinaire, Universite de Montreal, St-Hyacinthe, uebec, Canada.
authors.forename: M R
authors.initials: MR
authors.lastname: Shapouri
authors.forename: S K
authors.initials: SK
authors.lastname: Reddy
authors.forename: A
authors.initials: A
authors.lastname: Silim