The distribution of Paenibacillus larvae spores in adult bees and honey and larval mortality, following the addition of American foulbrood diseased brood or spore-contaminated honey in honey bee (Apis mellifera) colonies.
Within colony transmission of Paenibacillus larvae spores was studied by giving spore-contaminated honey comb or comb containing 100 larvae killed by American foulbrood to five experimental colonies respectively. We registered the impact of the two treatments on P. larvae spore loads in adult bees and honey and on larval mortality by culturing for spores in samples of adult bees and honey, respectively, and by measuring larval survival. The results demonstrate a direct effect of treatment on spore levels in adult bees and honey as well as on larval mortality. Colonies treated with dead larvae showed immediate high spore levels in adult bee samples, while the colonies treated with contaminated honey showed a comparable spore load but the effect was delayed until the bees started to utilize the honey at the end of the flight season. During the winter there was a build up of spores in the adult bees, which may increase the risk for infection in spring. The results confirm that contaminated honey can act as an environmental reservoir of P. larvae spores and suggest that less spores may be needed in honey, compared to in diseased brood, to produce clinically diseased colonies. The spore load in adult bee samples was significantly related to larval mortality but the spore load of honey samples was not.
Lindström
Anders
A
Department of Ecology, Swedish University of Agricultural Sciences, Uppsala, Sweden. anders.lindstrom@ekol.slu.se
Korpela
Seppo
S
Fries
Ingemar
I
D000818:Animals; D001406:Bacillaceae / Q000235:genetics / Q000302:isolation & purification*; D001516:Bees / Q000254:growth & development / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis; D018562:Disease Transmission, Infectious; D016908:Gram-Positive Bacterial Infections / Q000276:immunology / Q000382:microbiology / Q000662:veterinary*; D016937:Gram-Positive Endospore-Forming Rods / Q000472:pathogenicity* / Q000502:physiology; D006722:Honey / Q000382:microbiology*; D054884:Host-Pathogen Interactions; D007814:Larva / Q000382:microbiology; D008136:Longevity; D016133:Polymerase Chain Reaction / Q000379:methods; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013171:Spores, Bacterial / Q000302:isolation & purification / Q000472:pathogenicity*; D013997:Time Factors
D004269:DNA, Bacterial