title: Pan-dengue virus detection by PCR for travelers returning from the tropics.
abstract: We performed dengue virus (DENV) serology and quantitative real-time pan-DENV reverse transcription-PCR (RT-PCR) on 186 sera of 171 patients returning from the tropics. DENV loads significantly decreased with increasing times of disease and were higher in immunoglobulin M-negative samples. In the first week of disease, pan-DENV RT-PCR is the test of choice.
authors.affiliation: Department of Biomedicine, Division of Diagnostics, Institute for Medical Microbiology, University of Basel, Basel, Switzerland.
authors.forename: Alexis
authors.initials: A
authors.lastname: Dumoulin
authors.forename: Hanspeter
authors.initials: H
authors.lastname: Marti
authors.forename: Marcus
authors.initials: M
authors.lastname: Panning
authors.forename: Christoph
authors.initials: C
authors.lastname: Hatz
authors.forename: Hans H
authors.initials: HH
authors.lastname: Hirsch
mesh_terms: D000328:Adult; D001210:Asia, Southeastern; D003715:Dengue / Q000175:diagnosis*; D003716:Dengue Virus; D005189:False Positive Reactions; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013557:Switzerland; D014195:Travel