Determination of serum alcohol using a disposable biosensor.
This paper presents a disposable biosensor to detect serum alcohol concentration. The proposed biosensor is fabricated using the cross-linking method to immobilize Alcohol Dehydrogenase (ADH) and Nicotinamide Adenine Dinucleotide (NAD(+)) on the screen-printed electrode modified with Meldola's Blue (MB) absorbed on Nafion. It is based on the electrocatalytic properties of MB as an electron transfer mediator, which can catalyze the oxidation of NADH to NAD(+) at a low oxidizing potential, thus avoiding interferences due to the presence of oxidizable substances in the real serum samples. The biosensor response for alcohol is investigated in terms of pH, buffer solution, temperature and some interferents. It presents the good specificity, reproducibility, stability, accuracy and provides a fast response. The biosensor has been satisfactorily used for the measurement of serum alcohol.
Luo
Peng
P
Key Laboratory of Laboratory Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China.
Liu
Yi
Y
Xie
Guoming
G
Xiong
Xingliang
X
Deng
Shixiong
S
Song
Fangzhou
F
D000426:Alcohol Dehydrogenase / Q000737:chemistry; D001334:Automobile Driving / Q000331:legislation & jurisprudence; D015374:Biosensing Techniques / Q000295:instrumentation*; D002492:Central Nervous System Depressants / Q000097:blood*; D004566:Electrodes; D004579:Electron Transport; D000431:Ethanol / Q000097:blood*; D005465:Fluorocarbon Polymers; D053593:Forensic Toxicology; D006801:Humans; D009243:NAD / Q000737:chemistry; D010078:Oxazines; D010084:Oxidation-Reduction; D015203:Reproducibility of Results
D002492:Central Nervous System Depressants; D005465:Fluorocarbon Polymers; D010078:Oxazines; D009243:NAD; C040402:perfluorosulfonic acid; D000431:Ethanol; C005559:Meldola blue; D000426:Alcohol Dehydrogenase