title: Carbon nanotube field effect transistors for the fast and selective detection of human immunoglobulin G.
abstract: We report a field effect transistor (FET) based on a network of single-walled carbon nanotubes (SWCNTs) which can selectively detect human immunoglobulin G (HIgG). HIgG antibodies, which are strongly adsorbed onto the walls of the SWCNTs, are the basic elements of the recognition layer. The non-specific binding of proteins and the effects of other interferences are avoided by covering the non-adsorbed areas of the SWCNTs with Tween 20. The selectivity of the sensor has been tested against bovine serum albumin (BSA), the most abundant protein in plasma. HIgG in aqueous solution with concentrations from 1.25 mg L(-1) (8 nM) can be readily detected with response times of about 10 min. The SWCNT networks that form the basis of the sensor are easily grown by chemical vapour deposition. Silver screen-printed electrodes make the sensor quick to build. The sensitivity obtained with this sensor is similar to other FET devices based on SWCNTs built using much more complicated lithography processes. Moreover, the sensor is a reagentless device that does not need labels to detect HIgG.
authors.affiliation: Department of Analytical Chemistry and Organic Chemistry, Rovira i Virgili University, C/Marcel.lí Domingo s/n. 43007 Tarragona, Spain.
authors.forename: Cristina C
authors.initials: CC
authors.lastname: Cid
authors.forename: Jordi
authors.initials: J
authors.lastname: Riu
authors.forename: Alicia
authors.initials: A
authors.lastname: Maroto
authors.forename: F Xavier
authors.initials: FX
authors.lastname: Rius
mesh_terms: D004563:Electrochemistry / Q000295:instrumentation / Q000379:methods; D004566:Electrodes; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D037742:Nanotubes, Carbon; D012834:Silver; D014173:Transistors, Electronic
chemical_list: D007074:Immunoglobulin G; D037742:Nanotubes, Carbon; D012834:Silver