Salmonella Enteritidis-induced alteration of inflammatory CXCL chemokine messenger-RNA expression and histologic changes in the ceca of infected chicks.
To understand better the events in early avian host immune responses to Salmonella Enteritidis (SE), we examined messenger-RNA (mRNA) expression for eight genes: CXCLi1[K60], CXCLi2 [IL-8/CAF], interferon (IFN)-gamma, interleukin (IL)-1beta, IL-6, IL-12alpha, IL-12beta, and gallinacin (Gal)-2 in the ceca of young chicks 1 wk postinoculation with SE. Cecum tissue sections were stained and evaluated for the presence of macrophages, lymphocytes, heterophils, and apoptotic cells following SE infection. With the use of quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), SE infection was associated with a significant (P < 0.01) upregulation of cecal CXCLi1 and CXCLi2 mRNA expression. Infection with SE was also associated (P < 0.05) with increased staining for macrophages and decreased apoptosis (single-stranded DNA [ssDNA]) in cecal tissue sections when these sections were compared with those of uninfected animals. Changes in chemokine expression and cell population dynamics are a direct result of SE infection, as uninfected animals do not show these alterations. Thus, these SE-induced changes reflect the host immune response to SE in young chickens.
Cheeseman
Jennifer H
JH
Department of Animal Science, Iowa State University, Ames, IA 50011, USA.
Levy
Nyssa A
NA
Kaiser
Pete
P
Lillehoj
Hyun S
HS
Lamont
Susan J
SJ
D000818:Animals; D002432:Cecum / Q000276:immunology / Q000473:pathology; D019743:Chemokines, CXC / Q000235:genetics*; D002645:Chickens; D015870:Gene Expression; D008214:Lymphocytes / Q000473:pathology; D008264:Macrophages / Q000473:pathology; D011201:Poultry Diseases / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012481:Salmonella Infections, Animal / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D012477:Salmonella enteritidis / Q000276:immunology / Q000472:pathogenicity
D019743:Chemokines, CXC; D012333:RNA, Messenger