Controlled encapsulation of single-cells into monodisperse picolitre drops.
Encapsulation of cells within picolitre-size monodisperse drops provides new means to perform quantitative biological studies on a single-cell basis for large cell populations. Variability in the number of cells per drop due to stochastic cell loading is a major barrier to these techniques. We overcome this limitation by evenly spacing cells as they travel within a high aspect-ratio microchannel; cells enter the drop generator with the frequency of drop formation.
Edd
Jon F
JF
Massachusetts General Hospital, BioMEMS Resource Center, Charlestown, MA, USA.
Di Carlo
Dino
D
Humphry
Katherine J
KJ
Köster
Sarah
S
Irimia
Daniel
D
Weitz
David A
DA
Toner
Mehmet
M
D018922:HL-60 Cells; D006801:Humans; D008832:Microchemistry / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*