Mitochondrial respiratory chain and creatine kinase activities in rat brain after sepsis induced by cecal ligation and perforation.
The mechanisms responsible to the development of brain dysfunction during sepsis are not well understood. The objective of this study is to evaluate mitochondrial respiratory chain and creatine kinase activities in the brain after cecal ligation and perforation (CLP) in rats. We performed a prospective, controlled experiment in male Wistar rats. Rats were subjected to CLP (sepsis group) with saline resuscitation (at 50mL/kg immediately and 12h after cecal ligation and perforation) or sham operation (control group). Several times (0, 6, 12, 24, 48 and 96h) after CLP six rats were killed by decapitation, and brain structures (cerebellum, hippocampus, striatum and cortex) were isolated. Mitochondrial respiratory chain and creatine kinase activity were then measured. It was observed that animals submitted to CLP presented decreased mitochondrial respiratory chain activity in complex I, but not in complex II, III and IV, 24, 48 and 96h in all analyzed structures. Activity of succinate dehydrogenase was decreased in 48 and 96h in all analyzed structures. Creatine kinase activity increased after CLP in cerebellum, hippocampus and cortex (after 0h) and striatum (after 6h). Sepsis associated brain injury may include dysfunction in the mitochondrial respiratory chain activity.
Comim
Clarissa M
CM
Laboratório de Neurociências, Programa de Pós-Graduação Ciências da Saúde, Universidade do Extremo Sul Catarinense, 88806-000 Criciúma, SC, Brazil.
Rezin
Gislaine T
GT
Scaini
Giselli
G
Di-Pietro
Priscila B
PB
Cardoso
Mariane R
MR
Petronilho
Fabrícia C
FC
Ritter
Cristiane
C
Streck
Emilio L
EL
Quevedo
João
J
Dal-Pizzol
Felipe
F
D000818:Animals; D001921:Brain / Q000201:enzymology; D002432:Cecum / Q000502:physiology; D003402:Creatine Kinase / Q000378:metabolism*; D004579:Electron Transport / Q000187:drug effects; D008026:Ligation; D008297:Male; D008928:Mitochondria / Q000502:physiology*; D009043:Motor Activity; D051381:Rats; D017208:Rats, Wistar; D018805:Sepsis / Q000503:physiopathology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
D017392:Thiobarbituric Acid Reactive Substances; D003402:Creatine Kinase