[Effects of acrylonitrile on the activation of nuclear factor-kappaB signaling pathways and related gene expression in rat brain and rat glial cells].
OBJECTIVE
To study the effects of acrylonitrile on the activation of nuclear factor kappaB (NF-kappaB) signaling pathways and related gene expressions in rat brain and rat glial cells.


METHODS
The microarray and EMSA (electrophoretic mobility shift assay) techniques were used to investigated NF-kappaB pathway and related gene expressions changes in rat brain after treated with 0 mg/kg and 200mg/kg ACN for 14,28 and 90 days and rat normal glial cells after treated with 0, 25, 50 and 75 microg/ml ACN for 4 and 24 hours.


RESULTS
ACN induced the NF-kappaB pathway related gene expression (NIK, IKK, NF-kappaB1, NF-kappaB 2, IkappaBa, c-myc) in rat brain (in vivo) and in rat glial cells (in vitro). It was confirmed by EMSA results that ACN caused activation of the NF-kappaB pathway in rat brain and rat glial cells.


CONCLUSIONS
There is some relationship between gene expression of NF-kappaB pathway and ACN-induced oxidative stress.
Jiao
Zhenquan
Z
Office of Public Health Management, Chinese Center for Disease Control and Prevention, Beijing 100050, China. jiaozhenquan@hotmail.com
Pei
Xiaoyan
X
Guo
Yunchang
Y
Xu
Yong
Y
D000181:Acrylonitrile / Q000633:toxicity*; D000818:Animals; D001921:Brain / Q000166:cytology / Q000378:metabolism*; D002460:Cell Line; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015398:Signal Transduction / Q000187:drug effects*
D016328:NF-kappa B; D000181:Acrylonitrile