title: Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov.
abstract: Relationships among species assigned to the yeast genera Pichia, Issatchenkia and Williopsis, which are characterized by the ubiquinone CoQ-7 and inability to utilize methanol, were phylogenetically analyzed from nucleotide sequence divergence in the genes coding for large and small subunit rRNAs and for translation elongation factor-1alpha. From this analysis, the species separated into five clades. Species of Issatchenkia are members of the Pichia membranifaciens clade and are proposed for transfer to Pichia. Pichia dryadoides and Pichia quercuum are basal members of the genus Starmera. Williopsis species are dispersed among hat-spored taxa in each of the remaining three clades, which are proposed as the new genera Barnettozyma, Lindnera and Wickerhamomyces. Lineages previously classified as varieties of Pichia kluyveri, 'Issatchenkia'scutulata, Starmera amethionina and 'Williopsis'saturnus are elevated to species rank based on sequence comparisons.
authors.affiliation: Microbial Genomics and Bioprocessing Research Unit, National Center for Agricultural Utilization Research, US Department of Agriculture, Agricultural Research Service, Peoria, IL 61604, USA. cletus.kurtzman@ars.usda.gov
authors.forename: Cletus P
authors.initials: CP
authors.lastname: Kurtzman
authors.forename: Christie J
authors.initials: CJ
authors.lastname: Robnett
authors.forename: Eleanor
authors.initials: E
authors.lastname: Basehoar-Powers
mesh_terms: D004271:DNA, Fungal / Q000032:analysis / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D005800:Genes, Fungal / Q000235:genetics; D020459:Genes, rRNA / Q000235:genetics*; D016533:Mycological Typing Techniques; D020648:Peptide Elongation Factor 1 / Q000235:genetics*; D010802:Phylogeny; D010843:Pichia / Q000145:classification / Q000235:genetics; D012313:RNA / Q000235:genetics; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal / Q000235:genetics; D004718:Saccharomycetales / Q000145:classification / Q000235:genetics; D017422:Sequence Analysis, DNA; D013045:Species Specificity; D014451:Ubiquinone / Q000378:metabolism
chemical_list: D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D020648:Peptide Elongation Factor 1; D000077278:RNA, Mitochondrial; D012335:RNA, Ribosomal; D014451:Ubiquinone; D012313:RNA; C030777:ubiquinone 7