Interaction between Calpain 5, Peroxisome proliferator-activated receptor-gamma and Peroxisome proliferator-activated receptor-delta genes: a polygenic approach to obesity.
BACKGROUND
Obesity is a multifactorial disorder, that is, a disease determined by the combined effect of genes and environment. In this context, polygenic approaches are needed.


OBJECTIVE
To investigate the possibility of the existence of a crosstalk between the CALPAIN 10 homologue CALPAIN 5 and nuclear receptors of the peroxisome proliferator-activated receptors family.


METHODS
Cross-sectional, genetic association study and gene-gene interaction analysis.


METHODS
The study sample comprise 1953 individuals, 725 obese (defined as body mass index > or = 30) and 1228 non obese subjects.


RESULTS
In the monogenic analysis, only the peroxisome proliferator-activated receptor delta (PPARD) gene was associated with obesity (OR = 1.43 [1.04-1.97], p = 0.027). In addition, we have found a significant interaction between CAPN5 and PPARD genes (p = 0.038) that reduces the risk for obesity in a 55%.


CONCLUSIONS
Our results suggest that CAPN5 and PPARD gene products may also interact in vivo.
Sáez
María E
ME
Departamento de Genómica Estructural, Neocodex, C/.,Charles Darwin 6, Acc. A, 41092, Sevilla, Spain. mesaez@neocodex.es
Grilo
Antonio
A
Morón
Francisco J
FJ
Manzano
Luis
L
Martínez-Larrad
María T
MT
González-Pérez
Antonio
A
Serrano-Hernando
Javier
J
Ruiz
Agustín
A
Ramírez-Lorca
Reposo
R
Serrano-Ríos
Manuel
M
D002154:Calpain / Q000235:genetics*; D003430:Cross-Sectional Studies; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D009765:Obesity / Q000235:genetics* / Q000517:prevention & control*; D047494:PPAR delta / Q000235:genetics*; D047495:PPAR gamma / Q000235:genetics*; D011110:Polymorphism, Genetic
D047494:PPAR delta; D047495:PPAR gamma; D002154:Calpain; C494426:Capn5 protein, human