title: Localization and regional distribution of p23/TMP21 in the brain.
abstract: Sequential processing of amyloid precursor protein by beta- and gamma-secretases generates Alzheimer's disease (AD)-associated beta-amyloid peptides. Recently it was reported that the transmembrane protein p23/TMP21 associates with gamma-secretase, and negatively regulates beta-amyloid production. Despite the link between p23 function and AD pathogenesis, the expression of p23 has not been examined in the brain. Here, we describe the detailed immunohistochemical characterization of p23 expression in rodent and human brain. We report that p23 is co-expressed with gamma-secretase subunits in select neuronal cell populations in rodent brain. Interestingly, the steady-state level of p23 in the brain is high during embryonic development and then declines after birth. Furthermore, the steady-state p23 levels are reduced in the brains of individuals with AD. We conclude that p23 is expressed in neurons throughout the brain and the decline in p23 expression during postnatal development may significantly contribute to enhanced beta-amyloid production in the adult brain.
authors.affiliation: Department of Neurobiology, The University of Chicago, Chicago, IL 60637, USA.
authors.forename: Kulandaivelu S
authors.initials: KS
authors.lastname: Vetrivel
authors.forename: Anitha
authors.initials: A
authors.lastname: Kodam
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.forename: Angèle T
authors.initials: AT
authors.lastname: Parent
authors.forename: Satyabrata
authors.initials: S
authors.lastname: Kar
authors.forename: Gopal
authors.initials: G
authors.lastname: Thinakaran
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000831:Animals, Newborn; D001923:Brain Chemistry / Q000235:genetics; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D002531:Cerebellum / Q000737:chemistry / Q000378:metabolism; D005625:Frontal Lobe / Q000737:chemistry / Q000378:metabolism; D006367:HeLa Cells; D006624:Hippocampus / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008875:Middle Aged; D029543:Nucleocytoplasmic Transport Proteins; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D008565:Membrane Proteins; D029543:Nucleocytoplasmic Transport Proteins; C484395:TMED10 protein, human; C100672:Tmed10 protein, rat