title: CYP1A induction potential and the concentration of priority pollutants in marine sediment samples--in vitro evaluation using the PLHC-1 fish hepatoma cell line.
abstract: The use of in vitro biotests in combination with chemical determination of priority pollutants is considered a promising approach in environmental risk assessment. The main goal of this study was to evaluate the relationship between the CYP1A induction potential and the concentration of priority pollutants (PAHs, PCBs and heavy metals) in contaminated marine sediments. Six sediment samples characterized by different types of pollution were collected from the Bay of Kvarner, Croatia. CYP1A induction potency was determined in vitro by the measurement of ethoxyresorufin-O-deethylase (EROD) activity in PLHC-1 fish hepatoma cells. The results were compared to the potency of the model CYP1A inducer 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and expressed in 2,3,7,8-TCDD equivalents. All of the tested sediment samples were able to induce CYP1A activity in a dose-dependent manner. On a general scale, there was a good correlation between CYP1A induction and the concentration of priority pollutants in the tested samples. However, some samples, which had relatively low levels of priority pollutants, exhibited a strong CYP1A induction response. Therefore, apart from the confirmed usability and sensitivity of the EROD determination in the PLHC-1 cells as a suitable in vitro model in ecotoxicology, the results of this study indicate that the list of priority pollutants usually determined in the attempt to evaluate the risk of adverse effects to marine wildlife should be reconsidered.
authors.affiliation: Department of Environmental Medicine, Medical Faculty, University of Rijeka, Braće Branchetta 20a, Rijeka, Croatia. Luka.Traven@medri.hr
authors.forename: Luka
authors.initials: L
authors.lastname: Traven
authors.forename: Roko
authors.initials: R
authors.lastname: Zaja
authors.forename: Jovica
authors.initials: J
authors.lastname: Loncar
authors.forename: Tvrtko
authors.initials: T
authors.lastname: Smital
authors.forename: Vladimir
authors.initials: V
authors.lastname: Mićović
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000378:metabolism*; D045744:Cell Line, Tumor; D017523:Croatia; D019363:Cytochrome P-450 CYP1A1 / Q000096:biosynthesis / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods; D004790:Enzyme Induction; D019015:Geologic Sediments / Q000737:chemistry; D008113:Liver Neoplasms / Q000201:enzymology / Q000378:metabolism*; D000072317:Polychlorinated Dibenzodioxins / Q000008:administration & dosage / Q000633:toxicity; D018570:Risk Assessment / Q000379:methods; D014874:Water Pollutants, Chemical / Q000008:administration & dosage / Q000633:toxicity*
chemical_list: D000072317:Polychlorinated Dibenzodioxins; D014874:Water Pollutants, Chemical; D019363:Cytochrome P-450 CYP1A1