Transplacental transfer and metabolism of 17-alpha-hydroxyprogesterone caproate.
OBJECTIVE
Determine transplacental transfer and metabolism of 17-alpha-hydroxyprogesterone caproate and its distribution between the tissue and the maternal and fetal circuits of the dually perfused placental lobule.


METHODS
17-alpha-Hydroxyprogesterone caproate (21 ng/mL) and its dual-labeled isotope, 17-alpha-hydroxy-[(3)H] progesterone [(14)C] caproate were added to the maternal circuit. The concentrations of the drug and its metabolite in trophoblast tissue and both circuits were determined by high performance liquid chromatography and liquid scintillation spectrometry.


RESULTS
17-alpha-Hydroxyprogesterone caproate was transferred from the maternal to fetal circuit. After a 4-hour perfusion period, a metabolite of 17-alpha-hydroxyprogesterone caproate that retained both progesterone and caproate moieties was identified in the tissue and the maternal and fetal circuits. Neither 17-alpha-hydroxyprogesterone caproate nor its metabolite, at the concentrations tested, had adverse effect on determined viability and functional parameters of placental tissue.


CONCLUSIONS
17-alpha-Hydroxyprogesterone caproate was metabolized by term placental lobule during its perfusion and both parent compound and its metabolite(s) transferred to the fetal circuit.
Hemauer
Sarah J
SJ
Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston, TX, USA.
Yan
Ru
R
Patrikeeva
Svetlana L
SL
Mattison
Donald R
DR
Hankins
Gary D V
GD
Ahmed
Mahmoud S
MS
Nanovskaya
Tatiana N
TN
D000077713:17 alpha-Hydroxyprogesterone Caproate; D000328:Adult; D002851:Chromatography, High Pressure Liquid; D005260:Female; D006801:Humans; D006908:Hydroxyprogesterones / Q000378:metabolism*; D010101:Oxygen Consumption / Q000502:physiology; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D045167:Progesterone Congeners / Q000378:metabolism*; D014018:Tissue Distribution; D014022:Tissue Survival; D014327:Trophoblasts / Q000378:metabolism*
D006908:Hydroxyprogesterones; D045167:Progesterone Congeners; D000077713:17 alpha-Hydroxyprogesterone Caproate