Minimal traumatic brain injury induce apoptotic cell death in mice.
In the United States, 1.4 million people suffer from traumatic brain injury (TBI) each year because of traffic, sports, or war-related injuries. The majority of TBI victims suffer mild to minimal TBI (mTBI), but most are released undiagnosed. Detailed pathologies are poorly understood. We characterized the microscopic changes of neurons of closed-head mTBI mice after increased unilateral trauma using hematoxylin and eosin (H&E) stain, and correlated it with the expression of the apoptotic proteins c-jun, p53, and BCL-2. Minimal damage to the brain increases the number of pyknotic appearing neurons and activates the apoptotic proteins in both hemispheres. Although minimal, increased impact was positively correlated with the increased number of damaged neurons. These results may explain the wide variety of behavioral and cognitive deficits closed-head mTBI causes in mice. Our cumulative results point to the pathological origin of post-concussion syndrome and may aid in the development of future neuroprotective strategies for the disease.
Tashlykov
V
V
Laboratory of Anesthesia, Pain and Neural Research, Bruce Rapaport Medical Faculty, Technion Israel Institute of Technology, Haifa, Israel.
Katz
Y
Y
Volkov
A
A
Gazit
V
V
Schreiber
S
S
Zohar
O
O
Pick
C G
CG
D000818:Animals; D017209:Apoptosis; D015153:Blotting, Western; D001930:Brain Injuries / Q000473:pathology*; D004396:Coloring Agents; D018891:Dentate Gyrus / Q000473:pathology; D004801:Eosine Yellowish-(YS); D005625:Frontal Lobe / Q000473:pathology; D006179:Gyrus Cinguli / Q000473:pathology; D016489:Head Injuries, Closed / Q000473:pathology*; D006416:Hematoxylin; D007150:Immunohistochemistry; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D009474:Neurons / Q000473:pathology*; D012720:Severity of Illness Index
D004396:Coloring Agents; D004801:Eosine Yellowish-(YS); D006416:Hematoxylin