Age-related upregulation of Drosophila caudal gene via NF-kappaB in the adult posterior midgut.
The Drosophila midgut has emerged as a powerful model system for the investigation of fundamental cellular pathways relevant to intestinal stem cell biology. Understanding the age-related changes in the adult Drosophila midgut may provide insights into the molecular mechanisms that link aging to the modulation of adult stem cell population. The caudal-related homeobox genes encode intestine-specific transcription factors required for normal intestinal development and maintenance. Here, we demonstrate that caudal gene expression is upregulated in the adult posterior midgut in response to age and oxidative stress, and that overexpression of Caudal can stimulate cell proliferation in the adult posterior midgut. We further demonstrate that the age- and oxidative-stress-related upregulation of the caudal gene is mediated by the NF-kappaB binding site located in the 5'-flanking region of the caudal gene. Our results may contribute to an understanding of the mechanisms of age-related changes in the number and activity of intestinal stem cells and progenitors in the Drosophila adult midgut.
Choi
Yoon-Jeong
YJ
Department of Molecular Biology, College of Natural Science, Pusan National University, Busan 609-735, Republic of Korea.
Hwang
Mi-Sun
MS
Park
Joung-Sun
JS
Bae
Soo-Kyung
SK
Kim
Young-Shin
YS
Yoo
Mi-Ae
MA
D000375:Aging / Q000187:drug effects / Q000235:genetics*; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D002457:Cell Extracts; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D004064:Digestive System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004331:Drosophila melanogaster / Q000166:cytology / Q000187:drug effects / Q000235:genetics*; D017344:Genes, Insect; D018398:Homeodomain Proteins / Q000235:genetics*; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008969:Molecular Sequence Data; D016328:NF-kappa B / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics*
D002457:Cell Extracts; D029721:Drosophila Proteins; D018398:Homeodomain Proteins; D008070:Lipopolysaccharides; D016328:NF-kappa B; C101626:Rel protein, Drosophila; D014157:Transcription Factors; C084564:cad protein, Drosophila; D006861:Hydrogen Peroxide