Two new methods for preparing a unique stratum corneum substitute.
Stratum corneum lipids play an important role in the barrier function of the skin. An in vitro permeation model consisting of synthetic lipids has previously been developed to replace human stratum corneum (SC) in permeation studies. This model is referred to as the stratum corneum substitute (SCS). In order to improve its reproducibility and to increase the efficiency in preparing the SCS, two new preparation methods are developed. Subsequently the properties of the SCS prepared by the various methods, i.e. the manual airbrush method, the rotor airbrush method and the linomat method, are investigated. The results show that the SCS prepared with the various methods share the properties of a uniform lipid composition and lipid distribution. Furthermore, irrespective of the preparation method, the lipids form crystalline lamellar phases, mimicking the lipid organization and orientation in human SC. As a result, permeation profiles of benzoic acid through SCS are very similar to human SC. The rotor method increases the efficiency and reproducibility of the manual airbrush method, while the linomat method reduces the lipid loss during preparation and results in SCS with a more uniform membrane thickness. In conclusion, the linomat method was chosen as the preferred method for preparing the substitute.
Groen
Daniël
D
Leiden/Amsterdam Center for Drug Research, Department of Drug Delivery Technology, Gorlaeus Laboratories, University of Leiden, P.O. Box 9502, 2300 RA Leiden, The Netherlands.
Gooris
Gert S
GS
Ponec
Maria
M
Bouwstra
Joke A
JA
D004058:Diffusion; D006801:Humans; D008055:Lipids / Q000737:chemistry; D010539:Permeability; D012867:Skin / Q000033:anatomy & histology* / Q000737:chemistry; D017768:Skin, Artificial
D008055:Lipids