title: [Properties of post-proline cleaving enzymes from Tenebrio molitor].
abstract: Two post-proline cleaving enzymes PRE1 and PRE2 with molecular masses of 101 and 62 kDa, respectively, capable of hydrolyzing Z-AlaAlaPro-pNA were isolated for the first time from the midgut of the flour beetle Tenebrio molitor and characterized. PRE1 is active only in acidic media, with a maximum at pH 5.6, whereas PRE2, both in acidic and alkaline media with a maximum at pH 7.9. Using inhibitory analysis, both PRE1 and PRE2 were shown to belong to serine peptidases. Some data indicate that a Cys residue is located close to the PRE2 active site. Z-Pro-prolinal, a specific inhibitor of prolyl oligopeptidases, inhibits completely PRE2 and partially PRE1. The substrate specificities of the isolated enzymes were studied. It was shown that Z-AlaAla-Pro-pNA was the best substrate for PRE1, and Z-AlaPro-pNA, for PRE2. The combination of the studied properties allowed characterization of PRE2 as a prolyl oligopeptidase.
authors.forename: I A
authors.initials: IA
authors.lastname: Goptar'
authors.forename: I A
authors.initials: IA
authors.lastname: Kulemzina
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Filippova
authors.forename: E N
authors.initials: EN
authors.lastname: Lysogorskaia
authors.forename: E S
authors.initials: ES
authors.lastname: Oksenoĭt
authors.forename: D P
authors.initials: DP
authors.lastname: Zhuzhikov
authors.forename: Ia E
authors.initials: IaE
authors.lastname: Dunaevskiĭ
authors.forename: M A
authors.initials: MA
authors.lastname: Belozerskiĭ
authors.forename: E N
authors.initials: EN
authors.lastname: Elpidina
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D002850:Chromatography, Gel; D006863:Hydrogen-Ion Concentration; D006868:Hydrolysis; D007814:Larva / Q000201:enzymology; D000085822:Prolyl Oligopeptidases; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000302:isolation & purification; D013379:Substrate Specificity; D013712:Tenebrio / Q000201:enzymology*
chemical_list: D000814:Aniline Compounds; D012697:Serine Endopeptidases; D000085822:Prolyl Oligopeptidases