In vitro evaluation of freeze-dried bone allografts combined with platelet rich plasma and human bone marrow stromal cells for tissue engineering.
Freeze-dried bone allograft (FDBA) might be more effective in combination with platelet rich plasma (PRP) and bone marrow stromal cells (BMSC) in accelerating bone healing. The isolation of BMSC through density gradient (pBMSC) is not extensively applicable in clinical practice, because it increases the risk of infection. Alternatively, BMSC can be concentrated by simple centrifugation (wBMSC) directly in the operating room. However, we do not know if wBMSC act in the same way as pBMSC. BMSC from 10 donors were tested whether, in the presence of a combination of FDBA and autologous PRP, the osteogenic differentiation of the cells concentrated by simple centrifugation (wBMSC + FDBA + PRP) was similar to that of pBMSC. Cell-associated alkaline phosphatase, osterix and fibroblast growth factor-2 were higher in wBMSC + FDBA + PRP. In conclusion, the combination of FDBA and PRP had a favouring effect on the differentiation towards osteoblasts and allowed BMSC concentrated by simple centrifugation to differentiate as fast as BMSC purified by density gradient.
Cenni
Elisabetta
E
Laboratory for Pathophysiology of Orthopaedic Implants, Istituto Ortopedico Rizzoli, via di Barbiano 1/10, 40136, Bologna, Italy. elisabetta.cenni@ior.it
Perut
Francesca
F
Ciapetti
Gabriela
G
Savarino
Lucia
L
Dallari
Dante
D
Cenacchi
Annarita
A
Stagni
Cesare
C
Giunti
Armando
A
Fornasari
Pier Maria
PM
Baldini
Nicola
N
D000469:Alkaline Phosphatase / Q000235:genetics / Q000378:metabolism; D001483:Base Sequence; D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism; D018786:Bone Substitutes; D016025:Bone Transplantation / Q000379:methods*; D002454:Cell Differentiation; D002469:Cell Separation; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D017931:DNA Primers / Q000235:genetics; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism; D005612:Freeze Drying; D006801:Humans; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism; D015675:Osteocalcin / Q000235:genetics / Q000378:metabolism; D053657:Platelet-Rich Plasma; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000076182:Sp7 Transcription Factor; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism / Q000637:transplantation*; D023822:Tissue Engineering / Q000379:methods*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D014184:Transplantation, Homologous
D018786:Bone Substitutes; D050659:Core Binding Factor Alpha 1 Subunit; D017931:DNA Primers; D012333:RNA, Messenger; C493709:RUNX2 protein, human; D000076182:Sp7 Transcription Factor; C478163:SP7 protein, human; D014157:Transcription Factors; D016222:Fibroblast Growth Factor 2; D015675:Osteocalcin; D000469:Alkaline Phosphatase