[Expression of NR1 mRNA of NMDA receptor by gastrodine on hypoxia injury in cultured rat cerebral cortical neurons].
OBJECTIVE
To investigate the effect of gastrodine on the expression of NR1 mRNA of NMDA receptor in cultured rat cerebral cortical neurons injury induced by hypoxia.


METHODS
The injury models of cultured rat cerebral cortical neurons was made by hypoxia and hypoglucose. The concentration of 13, 26, 52 mg x L(-1) of gastrodine was respectively added to the injured cells. The R-PCR technique was used to study the expression of NR1 mRNA of NMDA receptor.


RESULTS
The expression of NR1 mRNA increased markedly in hypoxia and hypoglucose injured cells, the increased expression can be weakened by gastrodine, more significant effect was found in the concentration of 26 mg x L(-1) 52 mg x L(-1).


CONCLUSIONS
Gastrodine may act as a neuro - protector by weakening the expression of NR1 mRNA in cultured rat cerebral cortical neurons injury induced by hypoxia and hypoglucose.
Fu
Lei
L
Pharmacy College, Zengzhou University, Zhengzhou 450001, China. oldfu123@zzu.edu.cn
Mao
Yan-Hua
YH
Gao
Yuan
Y
Liu
Lei
L
Wang
Zhi-Ping
ZP
Li
Liang-Chen
LC
D000818:Animals; D001592:Benzyl Alcohols / Q000494:pharmacology*; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000166:cytology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D005960:Glucosides / Q000494:pharmacology*; D000860:Hypoxia / Q000235:genetics*; D009474:Neurons / Q000166:cytology* / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics*; D051381:Rats; D017208:Rats, Wistar; D016194:Receptors, N-Methyl-D-Aspartate / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D001592:Benzyl Alcohols; D005960:Glucosides; D012333:RNA, Messenger; D016194:Receptors, N-Methyl-D-Aspartate; C045345:gastrodin