Effects of combined pulse electromagnetic field stimulation plus glutamine on the healing of colonic anastomosis in rats.
OBJECTIVE
An experimental study was designed to investigate the effect of combined pulse electromagnetic field (PEMF) stimulation plus glutamine administration on colonic anastomosis.


METHODS
Anastomosis of the left colon was performed in 28 rats, which were divided into four groups; Group 1: normal resection anastomosis plus oral 50 mg/kg/day glutamine; Group 2: normal resection anastomosis plus PEMF stimulation plus oral 50 mg/kg/day glutamine; Group 3: normal resection anastomosis plus PEMF stimulation; Group 4: normal resection anastomosis. On the seventh postoperative day, the animals were killed and the bursting pressure and tissue hydroxyproline concentration of the anastomosis were analyzed and compared.


RESULTS
The mean anastomotic bursting pressure in Group 2 was significantly higher than in Groups 1 and 4. On the other hand, the mean anastomotic bursting pressure in Group 1 was significantly higher than in Group 4. The collagen deposition and the fibroblast infiltration were significantly increased on the seventh day in Group 3 compared the other groups. On the other hand, Groups 1 and 2 had higher scores for collagen deposition and fibroblast infiltration than Group 4.


CONCLUSIONS
In conclusion, burst pressures, hydroxyproline, and histologic features (fibroblast infiltration and collagen deposition) were improved in the PEMF group, and both PEMF and glutamine-enriched nutrition provide a significant gain in the strength of colonic anastomoses in rats.
Girgin
Sadullah
S
Department of General Surgery, Medical School, Dicle University, Diyarbakir, Turkey.
Gedik
Ercan
E
Ozturk
Hayrettin
H
Akpolat
Veysi
V
Akbulut
Veysi
V
Kale
Ebru
E
Buyukbayram
Huseyin
H
Celik
Salih
S
D000714:Anastomosis, Surgical; D000818:Animals; D001696:Biomechanical Phenomena; D003106:Colon / Q000473:pathology / Q000502:physiology / Q000601:surgery*; D005973:Glutamine / Q000627:therapeutic use*; D055909:Magnetic Field Therapy; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D014945:Wound Healing
D005973:Glutamine