Genetic characterization and phytochemical analysis of wild and cultivated populations of Scutellaria baicalensis.
Scutellaria baicalensis was collected from four wild and four cultivated populations from different locations in China. Forty-two samples were analyzed using random amplification of polymorphic DNA (RAPD) techniques for genetic profiling, and high performance liquid chromatography (HPLC) techniques to determine the flavonoid content. The selected 23 RAPD primers yielded a total of 838 clear and reproducible bands of which 237 were found to be polymorphic. The wild population exhibited higher polymorphism than that of the cultivated population. The dendrogram generated by the UPGMA method via Nei's genetic distance revealed three distinct genotypes from the cultivated populations and several branches from the wild populations. The contents of baicalin and wogonoside in dried roots of the samples ranged from (w/w) 8.63 to 17.84%, and from 1.99 to 4.21%, respectively, whereas their aglycones, baicalein and wogonin, were within the range of only 0.04-0.23%. The total content of the four flavonoids varied from 9.45 to 26.24%. Comparatively, the cultivated populations contained much higher levels of baicalin and total flavonoids than those in the wild populations. The results from genetic characterization and phytochemical analysis demonstrated that the quality variation of this drug was mainly determined by extrinsic environmental or agricultural factors, rather than by genetic differences. Our findings can be used for the commercial production and germplasm management of this medicinal plant.
Su
Su
S
Research Center of Natural Products, School of Life Sciences, Fudan University, Shanghai 200433, P. R. China.
He
Chang-Ming
CM
Li
Lin-Chu
LC
Chen
Jia-Kuan
JK
Zhou
Tong-Shui
TS
D002851:Chromatography, High Pressure Liquid; D044950:Flavanones / Q000032:analysis; D005419:Flavonoids / Q000032:analysis; D014644:Genetic Variation; D005960:Glucosides / Q000032:analysis; D019105:Random Amplified Polymorphic DNA Technique; D031364:Scutellaria baicalensis / Q000737:chemistry / Q000235:genetics*
D044950:Flavanones; D005419:Flavonoids; D005960:Glucosides; C038044:baicalin; C006680:baicalein; C473995:wogonoside; C085514:wogonin