title: The crossmatch may still be the most clinically relevant histocompatibility test performed.
abstract: We evaluated patient sera for flow PRA, FCXM, and end-point donor-antigen titer, and we correlated the results with graft survival. You cannot accurately predict a positive or negative FCXM result-not even when the sera have donor-specific antigens-unless you actually perform a crossmatch. Using fluorescence intensity as a surrogate for antibody concentration does not correlate quantitatively with the occurrence of a positive or negative crossmatch. Therefore, it is imperative to give each recipient a chance at being offered a donor organ by performance of a real-time crossmatch and not rely on a virtual evaluation.
authors.affiliation: Department of Surgery, Division of Immunology and Organ Transplantation, University of Texas Medical School, Houston, TX, USA.
authors.forename: Ronald
authors.initials: R
authors.lastname: Kerman
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Lappin
authors.forename: Barry
authors.initials: B
authors.lastname: Kahan
authors.forename: Stephen
authors.initials: S
authors.lastname: Katz
authors.forename: Eva
authors.initials: E
authors.lastname: McKissick
authors.forename: Kathleen
authors.initials: K
authors.lastname: Hosek
authors.forename: Noriel
authors.initials: N
authors.lastname: Acorda
authors.forename: Nicholas
authors.initials: N
authors.lastname: Wooley
authors.forename: Angela
authors.initials: A
authors.lastname: Hoover
authors.forename: Kristah
authors.initials: K
authors.lastname: Miller
authors.forename: Luis
authors.initials: L
authors.lastname: Rodriguez
authors.forename: Brandt
authors.initials: B
authors.lastname: Moore
authors.forename: Porsha
authors.initials: P
authors.lastname: Melcher
authors.forename: Bridget
authors.initials: B
authors.lastname: Biedermann
authors.forename: Charles
authors.initials: C
authors.lastname: Van Buren
mesh_terms: D005434:Flow Cytometry; D006084:Graft Rejection / Q000276:immunology; D006085:Graft Survival / Q000276:immunology*; D006650:Histocompatibility Testing / Q000379:methods*; D006801:Humans; D007163:Immunosorbent Techniques; D016030:Kidney Transplantation / Q000276:immunology*; D011237:Predictive Value of Tests; D012189:Retrospective Studies; D014181:Transplantation Immunology; D014184:Transplantation, Homologous