title: Evaluation of natural substances from Evolvulus alsinoides L. with the purpose of determining their antioxidant potency.
abstract: In recent years, great attention has been given to the search for natural compounds or extracts with the purpose of medical use. Evolvulus alsinoides L. (Convolvulaceae) is a plant used in traditional medicine of East Asia in many indications and has known nootropic and anti-inflammatory activity. However, the bioactive constituents have been described poorly in the literature. Four substances isolated from the ethanol extract of E. alsinoides by means of polyamide and Silica-gel chromatography are reported here. Their molecular structures were determined using NMR analyses. There were identified as scopoletin, umbelliferone, scopolin and 2-methyl-1,2,3,4-butanetetrol. The quantity of these substances was determined using HPLC-UV and GC-FID detection. Antioxidant activity of the isolated substances was measured by DPPH assay using the SIA method. Antioxidant activity and total phenolic content of the prepared fractions are also described. The prepared fractions and isolated substances did not exhibit any significant activity in DPPH test.
authors.affiliation: Department of Pharmaceutical Botany and Ecology, Faculty of Pharmacy, Charles University, Hradec Kralove, Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Cervenka
authors.forename: Vit
authors.initials: V
authors.lastname: Koleckar
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Rehakova
authors.forename: Ludek
authors.initials: L
authors.lastname: Jahodar
authors.forename: Jiri
authors.initials: J
authors.lastname: Kunes
authors.forename: Lubomir
authors.initials: L
authors.lastname: Opletal
authors.forename: Radomir
authors.initials: R
authors.lastname: Hyspler
authors.forename: Daniel
authors.initials: D
authors.lastname: Jun
authors.forename: Kamil
authors.initials: K
authors.lastname: Kuca
mesh_terms: D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D027722:Convolvulaceae / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts