A uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements.
A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied displacement. The device works in a fashion similar to that of a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless atomic force microscope (AFM) cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged by use of epifluorescence and phase contrast techniques.
Serrell
David B
DB
National Institute of Standards and Technology, Boulder, CO, USA. david.serrell@colorado.edu.|University of Colorado, Boulder, CO, USA. david.serrell@colorado.edu.
Law
Jera
J
National Institute of Standards and Technology, Boulder, CO, USA.
Slifka
Andrew J
AJ
National Institute of Standards and Technology, Boulder, CO, USA.
Mahajan
Roop L
RL
Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
Finch
Dudley S
DS
Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005347:Fibroblasts / Q000648:ultrastructure*; D046210:Microfluidic Analytical Techniques; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods