Granulocyte-colony stimulating factor increases donor mesenchymal stem cells in bone marrow and their mobilization into peripheral circulation but does not repair dystrophic heart after bone marrow transplantation.
BACKGROUND
Hereditary disordered cardiac muscle could be replaced with intact cardiomyocytes derived from genetically intact bone marrow (BM)-derived stem cells.


RESULTS
Cardiomyopathic mice with targeted mutation of delta-sarcoglycan gene underwent intra-BM-BM transplantation (IBM-BMT) from transgenic mice expressing green fluorescence protein. The host BM and the peripheral blood were completely reconstituted by donor-derived hematopoietic cells by IBM-BMT. Treatment with granulocyte-colony stimulating factor (G-CSF) markedly increased donor-derived mesenchymal stem cells (MSC) in the BM and their mobilization into the peripheral blood after IBM-BMT. Treatment with isoproterenol (iso) for 7 days caused myocardial damage and left ventricular (LV) dysfunction in the cardiomyopathic mice. Co-treatment with iso and G-CSF increased donor BM cell recruitment to the heart and temporarily improved LV function in the cardiomyopathic mice with or without IBM-BMT. However, the cardiac muscle was not replaced with donor BM-derived cardiomyocytes in the cardiomyopathic mice with or without IBM-BMT, and this was associated with no improvement of LV function of mice aged 20 weeks.


CONCLUSIONS
These results suggest that G-CSF enhances engraftment of donor MSC in the BM and their mobilization into the peripheral circulation after IBM-BMT but MSC recruited to the heart do not differentiate into cardiomyocytes and do not repair the dystrophic heart.
Tatsumi
Kimiko
K
The Department of Pediatrics, Kansai Medical University, Moriguchi, Japan.
Otani
Hajime
H
Sato
Daisuke
D
Enoki
Chiharu
C
Iwasaka
Toshiji
T
Imamura
Hiroji
H
Taniuchi
Shoichiro
S
Kaneko
Kazunari
K
Adachi
Yasushi
Y
Ikehara
Susumu
S
D000318:Adrenergic beta-Agonists / Q000494:pharmacology; D000818:Animals; D016026:Bone Marrow Transplantation; D009202:Cardiomyopathies / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D002454:Cell Differentiation / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D002478:Cells, Cultured; D004195:Disease Models, Animal; D005355:Fibrosis; D017930:Genes, Reporter; D016179:Granulocyte Colony-Stimulating Factor / Q000494:pharmacology*; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007545:Isoproterenol / Q000494:pharmacology; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000187:drug effects* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008822:Mice, Transgenic; D009136:Muscular Dystrophies / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology / Q000601:surgery*; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D010210:Papillary Muscles / Q000187:drug effects / Q000473:pathology; D049031:Sarcoglycans / Q000235:genetics / Q000378:metabolism; D013997:Time Factors; D016277:Ventricular Function, Left / Q000187:drug effects; D014916:Whole-Body Irradiation
D000318:Adrenergic beta-Agonists; D049031:Sarcoglycans; D016179:Granulocyte Colony-Stimulating Factor; D049452:Green Fluorescent Proteins; D007545:Isoproterenol