Evaluation of five clinical chemistry analyzers for use in health assessment in sea turtles.
OBJECTIVE
To compare blood biochemical values obtained from a handheld analyzer, 2 tabletop analyzers, and 2 diagnostic laboratories by use of replicate samples of sea turtle blood.


METHODS
Validation study.


METHODS
22 captive juvenile sea turtles.


METHODS
Sea turtles (18 loggerhead turtles [Caretta caretta], 3 green turtles [Chelonia mydas], and 1 Kemp's ridley turtle [Lepidochelys kempii]) were manually restrained, and a single blood sample was obtained from each turtle and divided for analysis by use of the 5 analyzers. Hematocrit and concentrations or activities of aspartate aminotransferase, creatine kinase, glucose, total protein, albumin, BUN, uric acid, P, Ca, K, Na, Cl, lactate dehydrogenase, and alkaline phosphatase were determined. Median values for each analyte were compared among the analyzers.


RESULTS
Significant differences were found among the analyzers for most values; however, data obtained from the 2 diagnostic laboratories were similar for all analytes. The magnitude of difference between the diagnostic laboratories and in-house units was > or = 10% for 10 of the 15 analytes.


CONCLUSIONS
Variance in the results could be attributed in part to differences in analyzer methodology. It is important to identify the specific methodology used when reporting and interpreting biochemical data. Depending on the variable and specific case, this magnitude of difference could conceivably influence patient management.
Wolf
Karen N
KN
Department of Clinical Sciences and Environmental Medicine Consortium, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27606, USA.
Harms
Craig A
CA
Beasley
Jean F
JF
D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D001800:Blood Specimen Collection / Q000295:instrumentation / Q000379:methods / Q000592:standards / Q000662:veterinary*; D012016:Reference Values; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014426:Turtles / Q000097:blood*