Technique of surface modification of a cell-adhesion-resistant hydrogel by a cell-adhesion-available inorganic microarray.
A microtransfer technique for micropattern fabrication using a dithiol macromolecular linker is suggested by transferring a conventionally photolithography-prepared gold microarray on a hard inorganic substrate to a polymeric substrate. The linker was synthesized by end-capping a poly(ethylene glycol) (PEG) chain by the thiol groups. The efficiency of this technique is demonstrated by the transfer of gold microdots from glass to a cell-adhesion-resistant PEG hydrogel, which was formed by polymerizing PEG diacrylate macromers. The stability and biocompatibility of the resulting polymeric-inorganic hybrid material and cell-adhesion contrast of the patterned surface is confirmed by preliminary cell experiments.
Sun
Jianguo
J
Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Department of Macromolecular Science, Fudan University, Shanghai 200433, China.
Graeter
Stefan V
SV
Yu
Lin
L
Duan
Shifeng
S
Spatz
Joachim P
JP
Ding
Jiandong
J
D000179:Acrylates / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion; D005898:Glass; D006046:Gold / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D007287:Inorganic Chemicals / Q000737:chemistry; D008422:Materials Testing; D008855:Microscopy, Electron, Scanning / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis; D011092:Polyethylene Glycols / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D040081:Protein Array Analysis; D013438:Sulfhydryl Compounds / Q000737:chemistry
D000179:Acrylates; D001672:Biocompatible Materials; D020100:Hydrogels; D007287:Inorganic Chemicals; D011108:Polymers; D013438:Sulfhydryl Compounds; D011092:Polyethylene Glycols; D006046:Gold