[Effect of nucleosomal binding protein 1 in androgen-independent prostatic carcinoma].
OBJECTIVE
To investigate biological function of nucleosomal binding protein 1 (NSBP1) in androgen-independent prostate cancer cells.


METHODS
Androgen-independent prostate cancer cells of the line PC-3 were cultured and transfected with recombinant inhibiting siRNA expression plasmid pSilencer 2. 1-U6 neo. Before transfection and 12, 36, 60, 84, 108 and 132 h after transfection RT-PCR and Western blotting was used to detect the inhibition effect. CCK-8 was used to detect the viability of the cells. The cell cycle was examined by flow cytometry.


RESULTS
The cell viability was significantly reduced since 60-108 h after the transfection (all P < 0.05), the inhibition ratio reaching climax 108 hours after the transfection (P = 0.028). However, the inhibition ratio began to increase. FC showed that since 60 h after the transfection the proportion of PC-3 cells at the G2M + S phase began to increased compared to that of the control group (all P < 0.05).


CONCLUSIONS
NSBP1 has important effect on maintaining cell viability and enhancing growth and proliferation of androgen-independent prostate cancer cells.
Huang
Chen
C
Department of Urology, Peking University First Hospital,Institute of Urology of Peking University, Beijing 100034, China.
Zhou
Li-Qun
LQ
Song
Gang
G
D015153:Blotting, Western; D002453:Cell Cycle; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002470:Cell Survival; D005434:Flow Cytometry; D024001:HMGN Proteins / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D008297:Male; D010957:Plasmids / Q000235:genetics; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015534:Trans-Activators / Q000235:genetics* / Q000378:metabolism*; D014162:Transfection
D024001:HMGN Proteins; C429289:HMGN5 protein, human; D034741:RNA, Small Interfering; D015534:Trans-Activators