Colonic perianastomotic carcinogenesis in an experimental model.
BACKGROUND
To examine the effect of anastomosis on experimental carcinogenesis in the colon of rats.


METHODS
Forty-three 10-week-old male and female Sprague-Dawley rats were operated on by performing an end-to-side ileorectostomy. Group A:16 rats received no treatment. Group B: 27 rats received 18 subcutaneous injections weekly at a dose of 21 mg/kg wt of 1-2 dimethylhydrazine (DMH), from the eighth day after the intervention. Animals were sacrificed between 25-27 weeks. The number of tumours, their localization, size and microscopic characteristics were recorded. A paired chi-squared analysis was performed comparing tumoral induction in the perianastomotic zone with the rest of colon with faeces.


RESULTS
No tumours appeared in the dimethylhydrazine-free group. The percentage tumoral area was greater in the perianastomotic zone compared to tumours which had developed in the rest of colon with faeces (p = 0.014).


CONCLUSIONS
We found a cocarcinogenic effect due to the creation of an anastomosis, when using an experimental model of colonic carcinogenesis induced by DMH in rats.
Pérez-Holanda
Sergio
S
Department of Surgery, Hospital Valle Del Nalón, Langreo, Asturias, Spain. sergio.perez-holanda@sespa.princast.es
Rodrigo
Luis
L
Pinyol-Felis
Carme
C
Vinyas-Salas
Joan
J
D019813:1,2-Dimethylhydrazine / Q000494:pharmacology; D000714:Anastomosis, Surgical; D000818:Animals; D002273:Carcinogens; D002277:Carcinoma / Q000139:chemically induced* / Q000473:pathology*; D003106:Colon / Q000187:drug effects / Q000473:pathology; D003110:Colonic Neoplasms / Q000139:chemically induced* / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D013997:Time Factors
D002273:Carcinogens; D019813:1,2-Dimethylhydrazine