Incorporation of beta-amyloid protein through the bovine ileal epithelium before and after weaning: model for orally transmitted amyloidoses.
To determine the mechanism of bovine intestinal incorporation of the pathogen, and the pathogenesis of prion protein in the early stage, cows suckling and weaning were orally given a fusion protein of Abeta-EGFP. Abeta-EGFP was incorporated through the villous columnar epithelial cells and accumulated in crypt patches in the ileum of suckling cows. The sites of the uptake and accumulation of Abeta-EGFP are very close to the peripheral nervous system; however, such uptake of Abeta-EGFP was not observed in 6-month-old post-weaning cows. The present study, therefore, suggests that the weaning period is very important for the risk of transmission.
Ano
Yasuhisa
Y
Department of Molecular Immunology, Graduate School of Argricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Nakayama
Hiroyuki
H
Sakai
Yusuke
Y
Sakudo
Akikazu
A
Endo
Maiko
M
Ebisu
Shogo
S
Li
JunYou
J
Uetsuka
Koji
K
Manabe
Noboru
N
Onodera
Takashi
T
D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000833:Animals, Suckling; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism / Q000635:transmission*; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007082:Ileum / Q000378:metabolism*; D007408:Intestinal Absorption; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000378:metabolism; D014886:Weaning
D016229:Amyloid beta-Peptides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins