Phase imaging of cells by simultaneous dual-wavelength reflection digital holography.
We present a phase-imaging technique to quantitatively study the three-dimensional structure of cells. The method, based on the simultaneous dual-wavelength digital holography, allows for higher axial range at which the unambiguous phase imaging can be performed. The technique is capable of nanometer axial resolution. The noise level, which increases as a result of using two wavelengths, is then reduced to the level of a single wavelength. The method compares favorably to software unwrapping, as the technique does not produce non-existent phase steps. Curvature mismatch between the reference and object beams is numerically compensated. The 3D images of SKOV-3 ovarian cancer cells are presented.
Khmaladze
Alexander
A
Department of Physics, University of South Florida, Tampa, FL 33620, USA. akhmalad@mail.usf.edu
Kim
Myung
M
Lo
Chun-Min
CM
D045744:Cell Line, Tumor; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D006696:Holography / Q000295:instrumentation*; D006801:Humans; D007089:Image Enhancement / Q000295:instrumentation; D007090:Image Interpretation, Computer-Assisted / Q000295:instrumentation; D021621:Imaging, Three-Dimensional / Q000295:instrumentation*; D007368:Interferometry / Q000295:instrumentation*; D008853:Microscopy / Q000295:instrumentation*; D010051:Ovarian Neoplasms / Q000473:pathology*; D010783:Photometry / Q000295:instrumentation*; D012815:Signal Processing, Computer-Assisted / Q000295:instrumentation*