title: Incorporation of beta-amyloid protein through the bovine ileal epithelium before and after weaning: model for orally transmitted amyloidoses.
abstract: To determine the mechanism of bovine intestinal incorporation of the pathogen, and the pathogenesis of prion protein in the early stage, cows suckling and weaning were orally given a fusion protein of Abeta-EGFP. Abeta-EGFP was incorporated through the villous columnar epithelial cells and accumulated in crypt patches in the ileum of suckling cows. The sites of the uptake and accumulation of Abeta-EGFP are very close to the peripheral nervous system; however, such uptake of Abeta-EGFP was not observed in 6-month-old post-weaning cows. The present study, therefore, suggests that the weaning period is very important for the risk of transmission.
authors.affiliation: Department of Molecular Immunology, Graduate School of Argricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
authors.forename: Yasuhisa
authors.initials: Y
authors.lastname: Ano
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakayama
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Sakai
authors.forename: Akikazu
authors.initials: A
authors.lastname: Sakudo
authors.forename: Maiko
authors.initials: M
authors.lastname: Endo
authors.forename: Shogo
authors.initials: S
authors.lastname: Ebisu
authors.forename: JunYou
authors.initials: J
authors.lastname: Li
authors.forename: Koji
authors.initials: K
authors.lastname: Uetsuka
authors.forename: Noboru
authors.initials: N
authors.lastname: Manabe
authors.forename: Takashi
authors.initials: T
authors.lastname: Onodera
mesh_terms: D016229:Amyloid beta-Peptides / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D000833:Animals, Suckling; D002417:Cattle; D016643:Encephalopathy, Bovine Spongiform / Q000378:metabolism / Q000635:transmission*; D004848:Epithelium / Q000378:metabolism*; D005260:Female; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D007082:Ileum / Q000378:metabolism*; D007408:Intestinal Absorption; D011993:Recombinant Fusion Proteins / Q000008:administration & dosage / Q000378:metabolism; D014886:Weaning
chemical_list: D016229:Amyloid beta-Peptides; D011993:Recombinant Fusion Proteins; D049452:Green Fluorescent Proteins