title: Effect of leukemia inhibitory factor (LIF) on the quality of in vitro produced mouse blastocysts and subsequent derivation of embryonic stem (ES) cells.
abstract: OBJECTIVE
  To determine the effect of leukemia inhibitory factor (LIF) on the quality of in vitro produced mouse blastocyst and the efficiency of embryonic stem (ES) cell derivation.
  
  
  METHODS
  Experimental study
  
  
  METHODS
  Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University
  
  
  METHODS
  In vivo fertilized zygotes were collected and subjected to in vitro culture in potassium simplex optimized medium (KSOM) containing 1,000 unit/ml LIF. The developmental ability of the zygote to blastocyst-stage and the cell numbers in blastocysts were evaluated Expanded blastocysts developed in different culture media were subsequently subjected to ES cell derivation. MAIN OUTCOME MEASURE (s): The influence of LIF on the quality of and the total cell numbers of blastocyst developed in vitro.
  
  
  RESULTS
  Supplementation of LIF in KSOM increased the rate of hatching blastocysts (63.8% vs. 53.7%; p < 0.05) and total cell numbers (91.4 +/- 15.0 vs. 85.1 +/- 7.7; p < 0.05) compared to KSOM alone. ES cells were obtained 66.7% from blastocysts developed in KSOM-LIF versus 41.7% in KSOM (p > 0.05). Established ES cell lines showed typical colony and characteristics of pluripotent murine ES cells.
  
  
  CONCLUSIONS
  LIF improved the quality of in vitro produced blastocysts but not enhanced ES cell derivation.
authors.affiliation: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: Ruttachuk
authors.initials: R
authors.lastname: Rungsiwiwut
authors.forename: Sasitorn
authors.initials: S
authors.lastname: Rungarunlert
authors.forename: Pranee
authors.initials: P
authors.lastname: Numchaisrika
authors.forename: Kamthorn
authors.initials: K
authors.lastname: Pruksananonda
authors.forename: Mongkol
authors.initials: M
authors.lastname: Techakumphu
authors.forename: Pramuan
authors.initials: P
authors.lastname: Virutamasen
mesh_terms: D000818:Animals; D001755:Blastocyst; D047108:Embryonic Development; D053595:Embryonic Stem Cells; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D053605:Leukemia Inhibitory Factor; D051379:Mice; D015053:Zygote
chemical_list: D053605:Leukemia Inhibitory Factor