title: Liquid chromatography tandem mass spectrometry method for the quantification of amisulpride with LLOQ of 100 pg/mL using 100 microL of plasma.
abstract: A sensitive and selective high-performance liquid chromatography-positive ion electrospray tandem mass spectrometry method was developed and validated for the quantification of amisulpride in 100 microL of human plasma. Following liquid-liquid extraction, the analytes were separated using an isocratic mobile phase on a reverse-phase column and analyzed by MS/MS in the multiple reaction monitoring mode using the respective (M + H)(+) ions, m/z 370-242 for amisulpride and m/z 341-112 for the internal standard. The assay exhibited a linear dynamic range with a lower range of 0.1-100 ng/mL and a higher range of 1-500 ng/mL of amisulpride in human plasma. The lower limit of quantification was 0.1 ng/mL with a relative standard deviation of less than 10%. Acceptable precision and accuracy were obtained for both linearity ranges. A run time of 2.0 min for each sample made it possible to analyze more than 275 human plasma samples per day. The validated method has been successfully used to analyze plasma samples for application in pharmacokinetic studies.
authors.affiliation: Pharmacokinetics and Drug Metabolism, Drug Discovery, Suven Life Sciences Ltd, Serene Chambers, Road 5, Avenue 7, Banjara Hills, Hyderabad 500034, India. ramakrishna_nirogi@yahoo.co.in
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Nirogi
authors.forename: Gopinadh
authors.initials: G
authors.lastname: Bhyrapuneni
authors.forename: Vishwottam
authors.initials: V
authors.lastname: Kandikere
authors.forename: Koteshwara
authors.initials: K
authors.lastname: Mudigonda
authors.forename: Devender
authors.initials: D
authors.lastname: Ajjala
authors.forename: Ramakrishna
authors.initials: R
authors.lastname: Suraneni
authors.forename: K
authors.initials: K
authors.lastname: Mukkanti
mesh_terms: D000077582:Amisulpride; D002853:Chromatography, Liquid / Q000379:methods*; D018492:Dopamine Antagonists / Q000097:blood / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D013469:Sulpiride / Q000031:analogs & derivatives* / Q000097:blood / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D018492:Dopamine Antagonists; D013469:Sulpiride; D000077582:Amisulpride