title: Nod2 mediates susceptibility to Yersinia pseudotuberculosis in mice.
abstract: Nucleotide oligomerisation domain 2 (NOD2) is a component of the innate immunity known to be involved in the homeostasis of Peyer patches (PPs) in mice. However, little is known about its role during gut infection in vivo. Yersinia pseudotuberculosis is an enteropathogen causing gastroenteritis, adenolymphitis and septicaemia which is able to invade its host through PPs. We investigated the role of Nod2 during Y. pseudotuberculosis infection. Death was delayed in Nod2 deleted and Crohn's disease associated Nod2 mutated mice orogastrically inoculated with Y. pseudotuberculosis. In PPs, the local immune response was characterized by a higher KC level and a more intense infiltration by neutrophils and macrophages. The apoptotic and bacterial cell counts were decreased. Finally, Nod2 deleted mice had a lower systemic bacterial dissemination and less damage of the haematopoeitic organs. This resistance phenotype was lost in case of intraperitoneal infection. We concluded that Nod2 contributes to the susceptibility to Y. pseudotuberculosis in mice.
authors.affiliation: INSERM, U843, Université Paris 7, Hôpital Robert Debré, Paris, France.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Meinzer
authors.forename: Sophie
authors.initials: S
authors.lastname: Esmiol-Welterlin
authors.forename: Frederick
authors.initials: F
authors.lastname: Barreau
authors.forename: Dominique
authors.initials: D
authors.lastname: Berrebi
authors.forename: Monique
authors.initials: M
authors.lastname: Dussaillant
authors.forename: Stephane
authors.initials: S
authors.lastname: Bonacorsi
authors.forename: Fabrice
authors.initials: F
authors.lastname: Chareyre
authors.forename: Michiko
authors.initials: M
authors.lastname: Niwa-Kawakita
authors.forename: Corinne
authors.initials: C
authors.lastname: Alberti
authors.forename: Ghislaine
authors.initials: G
authors.lastname: Sterkers
authors.forename: Claude
authors.initials: C
authors.lastname: Villard
authors.forename: Thecla
authors.initials: T
authors.lastname: Lesuffleur
authors.forename: Michel
authors.initials: M
authors.lastname: Peuchmaur
authors.forename: Michael
authors.initials: M
authors.lastname: Karin
authors.forename: Lars
authors.initials: L
authors.lastname: Eckmann
authors.forename: Marco
authors.initials: M
authors.lastname: Giovannini
authors.forename: Vincent
authors.initials: V
authors.lastname: Ollendorff
authors.forename: Hans
authors.initials: H
authors.lastname: Wolf-Watz
authors.forename: Jean-Pierre
authors.initials: JP
authors.lastname: Hugot
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism; D004198:Disease Susceptibility; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease; D006706:Homeostasis; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics* / Q000502:physiology*; D010581:Peyer's Patches / Q000382:microbiology*; D010641:Phenotype; D015011:Yersinia pseudotuberculosis / Q000235:genetics*; D015012:Yersinia pseudotuberculosis Infections / Q000235:genetics* / Q000382:microbiology*
chemical_list: D053473:Nod2 Signaling Adaptor Protein; C495945:Nod2 protein, mouse