title: Host response to implanted porcine-derived biologic materials in a primate model of abdominal wall repair.
abstract: Three commercially available porcine-derived biologic meshes were implanted in an Old World primate abdominal wall resection repair model to compare biological outcome as a predictor of clinical efficacy. Tissues were explanted over a 6-month period and evaluated for gross pathology, wound healing strength, mesenchymal cellular repopulation, vascularity, and immune response. In vivo functional outcomes were correlated with in vitro profile for each material. Small intestinal submucosa-based implants demonstrated scar tissue formation and contraction, coincident with mesh pleating, and were characterized by immediate and significant cellular and humoral inflammatory responses. Porcine dermal-based grafts demonstrated significant graft pleating, minimal integration, and an absence of cellular repopulation and vascularization. However, a significant cellular immune response surrounded the grafts, coincident with poor initial wound healing strengths. In vivo observations for the three porcine-derived mesh products correlated with individual in vitro profiles, indicating an absence of characteristic biochemical markers and structural integrity. This correlation suggests that in vivo results observed for these mesh products are a direct consequence of specific manufacturing processes that yield modified collagen matrices. The resulting loss of biological and structural integrity elicits a foreign body response while hindering normal healing and tissue integration.
authors.affiliation: LifeCell Corporation, Branchburg, New Jersey 08876, USA. msandor@lifecell.com
authors.forename: Maryellen
authors.initials: M
authors.lastname: Sandor
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.forename: Jerome
authors.initials: J
authors.lastname: Connor
authors.forename: Jared
authors.initials: J
authors.lastname: Lombardi
authors.forename: John R
authors.initials: JR
authors.lastname: Harper
authors.forename: Ronald P
authors.initials: RP
authors.lastname: Silverman
authors.forename: David J
authors.initials: DJ
authors.lastname: McQuillan
mesh_terms: D034861:Abdominal Wall / Q000473:pathology*; D000818:Animals; D000906:Antibodies; D000917:Antibody Formation / Q000276:immunology; D001672:Biocompatible Materials / Q000378:metabolism*; D001696:Biomechanical Phenomena; D004195:Disease Models, Animal; D020007:Implants, Experimental; D011182:Postoperative Care; D011323:Primates / Q000276:immunology*; D019919:Prosthesis Implantation; D034421:Sus scrofa / Q000378:metabolism*; D017186:Titrimetry; D014945:Wound Healing
chemical_list: D000906:Antibodies; D001672:Biocompatible Materials