title: Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription.
abstract: We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.
authors.affiliation: Nano Medical Engineering Laboratory, Advanced Science Institute, RIKEN 2-1, Hirosawa, Wako-Shi, Saitama 351-0198, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Furukawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Abe
authors.forename: Naoko
authors.initials: N
authors.lastname: Abe
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Harada
authors.forename: Satoshi
authors.initials: S
authors.lastname: Tsuneda
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Ito
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001483:Base Sequence; D012321:DNA-Directed RNA Polymerases / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology; D005456:Fluorescent Dyes / Q000494:pharmacology*; D008969:Molecular Sequence Data; D009690:Nucleic Acid Conformation; D010766:Phosphorylation; D012313:RNA / Q000737:chemistry; D012091:Repetitive Sequences, Nucleic Acid; D012394:Rosaniline Dyes / Q000737:chemistry*; D013050:Spectrometry, Fluorescence / Q000379:methods; D020080:Tandem Repeat Sequences; D014158:Transcription, Genetic; D014764:Viral Proteins / Q000378:metabolism
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D012394:Rosaniline Dyes; D014764:Viral Proteins; C005095:malachite green; D012313:RNA; C062210:bacteriophage T7 RNA polymerase; D012321:DNA-Directed RNA Polymerases