Induction of glucokinase in chicken liver by dietary carbohydrates.
We recently provided evidence of the presence of glucokinase (GCK) in the chicken liver [Berradi, H., Taouis, M., Cassy, S., Rideau, N., 2005. Glucokinase in chicken (Gallus gallus). Partial cDNA cloning, immunodetection and activity determination. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 141, 129-139]. In the present study we addressed the question of whether nutritional regulation of GCK occurs. Several nutritional conditions were compared in chickens (5 weeks old) previously trained to meal-feeding. One group was left in the fasted state (F: 24h) and one was tested at the end of the 2h meal (refed: RF). Two other 2h meal-refed groups received an acute oral saccharose load (6ml/kg BW) just before the 2h meal and were sacrificed either at the end of the meal (Saccharose refed, SRF) or 3h later (SRF+3). Liver GCK mRNA and protein levels did not differ between F, RF and SRF chickens but were significantly increased in SRF+3 chickens (2-fold, p<0.05). GCK activity did not differ between F and RF chickens but increased significantly in SRF and SRF+3 chickens (1.7-fold, p<0.05). Chicken liver GCK expression (mRNA and protein) and activity were therefore inducible in these chickens by feeding a meal with acute oral administration of carbohydrate. These and recent findings demonstrating insulin dependency of the liver GCK mRNA and protein strongly suggest that GCK may have an important role in carbohydrate metabolism, including that of the chicken. However, even in these highly stimulatory conditions, liver GCK activity remained relatively low in comparison with other species. The latter result may partly explain the high plasma glucose level in the chicken.
Rideau
Nicole
N
INRA, UR83 Recherches Avicoles, F-37380 Nouzilly, France. rideau@tours.inra.fr
Berradi
Hanaâ
H
Skiba-Cassy
Sandrine
S
Panserat
Stéphane
S
Cailleau-Audouin
Estelle
E
Dupont
Joëlle
J
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D015153:Blotting, Western / Q000662:veterinary; D001835:Body Weight / Q000187:drug effects / Q000502:physiology; D002645:Chickens / Q000378:metabolism*; D004040:Dietary Carbohydrates / Q000008:administration & dosage*; D004435:Eating / Q000187:drug effects / Q000502:physiology; D004790:Enzyme Induction / Q000187:drug effects; D005941:Glucokinase / Q000096:biosynthesis* / Q000235:genetics; D007328:Insulin / Q000097:blood / Q000378:metabolism; D007700:Kinetics; D008099:Liver / Q000187:drug effects* / Q000201:enzymology*; D008297:Male; D009929:Organ Size / Q000187:drug effects / Q000502:physiology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000662:veterinary; D018709:Statistics, Nonparametric
D001786:Blood Glucose; D004040:Dietary Carbohydrates; D007328:Insulin; D012333:RNA, Messenger; D005941:Glucokinase