Crystallization and preliminary characterization of arabis mosaic virus.
Arabis mosaic virus, a member of the nepovirus group, was purified by a combination of differential centrifugation, gel filtration, and sucrose density gradient techniques. The molecular weight of the coat protein, estimated by SDS-polyacrylamide gel electrophoresis, and consideration of subunit packing indicated the surface lattice of the virion to be T = 1. The virus was stable to treatment with trypsin at neutral pH. The virus crystallizes in the cubic system, space group P2(1)3 with a = 387 A . There are four particles per unit cell, each situated on a crystallographic threefold axis.
Takemoto
Y
Y
Faculty of Engineering, Tottori University, Koyama-cho, Tottori 680, Japan.
Nagahara
Y
Y
Fukuyama
K
K
Tsukihara
T
T
Iwaki
M
M