Determination of physiological thiols by electrochemical detection with piazselenole and its application in rat breast cancer cells 4T-1.
Glutathione (GSH), the most abundant cellular thiol, has been shown to play an important role in maintaining cellular redox equilibrium that is pivotal for cell growth and function. In the present paper a novel electrochemical probe of piazselenole containing a Se-N bond was well developed for the determination of GSH. The cyclic voltammogram of piazselenole scanned at 100 mV/s displayed an irreversible reduction peak at -0.106 V (vs Ag/AgCl electrode) and a significant peak current decrease could be further provoked with the addition of GSH into piazselenole solution. On the basis of the peak current decrease of piazselenole recorded by differential pulse voltammetry with the increase of GSH concentration, a working curve was constructed for GSH determination in the range of 5.0 x 10(-10) approximately 2.2 x 10(-8) M with the linear regression equation as DeltaiP (10(-6)A) = 0.0952 + 0.4287 x CGSH (10(-8) M) and the detection limit (3sigma) as 83 pM. The proposed method was satisfactorily applied to the extracts of rat breast cancer cells 4T-1 for intracellular thiols detection.
Wang
Wei
W
Key Laboratory of Eco-chemical Engineering of Ministry of Education,College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
Li
Lin
L
Liu
Shufeng
S
Ma
Cuiping
C
Zhang
Shusheng
S
D000818:Animals; D001393:Azoles / Q000737:chemistry*; D001943:Breast Neoplasms / Q000737:chemistry*; D045744:Cell Line, Tumor; D004563:Electrochemistry; D004566:Electrodes; D005456:Fluorescent Dyes / Q000737:chemistry*; D005978:Glutathione / Q000032:analysis; D006046:Gold / Q000737:chemistry; D015394:Molecular Structure; D016566:Organoselenium Compounds / Q000737:chemistry*; D010084:Oxidation-Reduction; D051381:Rats; D012680:Sensitivity and Specificity; D013438:Sulfhydryl Compounds / Q000032:analysis*
D001393:Azoles; D005456:Fluorescent Dyes; D016566:Organoselenium Compounds; D013438:Sulfhydryl Compounds; C042817:piaselenole; D006046:Gold; D005978:Glutathione