[Elaboration of the method for assessment of susceptibility to microbial growth of materials contacting with drinking water].
Initial methodological study was conducted to determine the susceptibility to microbial growth of different materials applied in contact with drinking water. The purpose of this assay was to elaborate the method for determining the ability of different materials contacting with drinking water to promote microbial growth and to determine possible correlation of results obtained by two analytic methods: inoculation of microorganisms swabbed from materials surface into a medium and ATP level examination. The assay was conducted during 16 weeks in dynamic conditions using continuous flow reactor. Every two weeks swabbes from examinated materials were collected and the total number of microorganisms was determined after incubation in 22 degrees C for 72 hours and in 37 degrees C for 48 hours respectively. Determination of microorganisms number and ATP level were both examinated in water inlet. Difference in susceptibility to microbial growth of different materials used in contact with drinking water was observed. This was confirmed by both analytic methods. Microbial growth on the surface of negative control materials (glass, poliethylene and stainless steel) was several time less intensive than on positive control material (floor finish not destined for contact with drinking water). Correlation between the quantity of microorganisms and ATP level on the surface of the same kind of materials was confirmed. Usefulness of continous flow reactor was confirmed.
Szczotko
Maciej
M
Zakład Higieny Komunalnej, Narodowy Instytut Zdrowia Publicznego, Państwowy Zakład Higieny, 00-791 Warszawa, ul Chocimska 24. mszczotko@pzh.gov.pl
Krogulska
Bozena
B
Krogulski
Adam
A
D001419:Bacteria / Q000254:growth & development*; D018407:Bacterial Physiological Phenomena; D015169:Colony Count, Microbial; D004866:Equipment Contamination; D005434:Flow Cytometry; D018857:Food Packaging; D005618:Fresh Water / Q000032:analysis*; D006801:Humans; D014871:Water Microbiology; D014881:Water Supply / Q000032:analysis*