title: Direct immobilization of cupredoxin azurin modified by site-directed mutagenesis on gold surface.
abstract: For making efficient bioelectronic device, we have developed novel immobilization method of cupredoxin azurin modified on gold (Au) surface. A recombinant protein with cysteine residue by using site-directed mutagenesis was designed and then directly immobilized on Au surface without any chemical linker. The immobilization of the functionalized protein is confirmed by surface plasmon resonance (SPR) and its surface morphology is analyzed by scanning tunneling microscopy (STM). The immobilization efficiency has been increased about 75.6%, as compared to that of wild-type azurin. The electrochemical property of the fabricated thin film was investigated by the cyclic voltammetry (CV). As a result, cysteine-modified azurin can be used for making high-quality protein film, and applied to the fabrication of nano-scale bioelectronics.
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
authors.forename: Sang-Uk
authors.initials: SU
authors.lastname: Kim
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Kim
authors.forename: Shin-Geon
authors.initials: SG
authors.lastname: Choi
authors.forename: Cheol-Heon
authors.initials: CH
authors.lastname: Yea
authors.forename: Ravindra P
authors.initials: RP
authors.lastname: Singh
authors.forename: Junhong
authors.initials: J
authors.lastname: Min
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Oh
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D000327:Adsorption; D001400:Azurin / Q000737:chemistry / Q000235:genetics; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics; D020349:Surface Plasmon Resonance / Q000379:methods*
chemical_list: C050107:cupredoxin; D001400:Azurin; D006046:Gold