[Glycolytic activity of enzyme preparation from the red king crab (Paralithodes camtschaticus) hepatopancreas].
Enzyme preparation exhibiting glycolytic activity yielding chitooligosaccharides along with N-acetyl-D-glucosamine was obtained from the red king crab (Paralithodes camtschaticus) hepatopancreas. The results of the analysis confirmed the presence of endo- and exochitinase activities in the preparation. HPLC showed that the hydrolysis products of chitin and chitosan did not contain D(+)-glucosamine, which is indicative of the absence of deacetylase and, apparently, exochitosanase activities. A comparison of the dependence of the enzyme preparation activity on temperature and pH of the incubation medium suggests that chitinase and protease activities are exhibited by different enzymes.
Rysakova
K S
KS
Novikov
V Iu
VIu
Mukhin
V A
VA
Serafimchik
E M
EM
D000117:Acetylglucosamine / Q000737:chemistry; D000818:Animals; D033381:Anomura / Q000201:enzymology*; D002688:Chitinases / Q000737:chemistry* / Q000302:isolation & purification; D043143:Hepatopancreas / Q000201:enzymology*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D010447:Peptide Hydrolases / Q000737:chemistry* / Q000302:isolation & purification; D013379:Substrate Specificity
D002688:Chitinases; D010447:Peptide Hydrolases; D000117:Acetylglucosamine