title: Characterization of soluble complexes of the Shigella flexneri type III secretion system ATPase.
abstract: The conserved ATPase of type III secretion systems is critical to the export of substrates through the apparatus. We present a characterization of the native T3SS ATPase, Spa47, from the cytoplasm of Shigella flexneri, demonstrating it to be in two distinct high-molecular-weight complexes with Spa33: MxiN and MxiK.
authors.affiliation: Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.
authors.forename: Steven
authors.initials: S
authors.lastname: Johnson
authors.forename: Ariel
authors.initials: A
authors.lastname: Blocker
mesh_terms: D000251:Adenosine Triphosphatases / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D002846:Chromatography, Affinity; D002850:Chromatography, Gel; D026901:Membrane Transport Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008970:Molecular Weight; D011485:Protein Binding; D012763:Shigella flexneri / Q000201:enzymology*
chemical_list: D026901:Membrane Transport Proteins; D000251:Adenosine Triphosphatases; C478167:Spa47 protein, Shigella flexneri