An operative approach to correct CD spectra distortions due to absorption flattening.
We discuss the influence of absorption flattening (AF) on CD spectra, aiming at finding a simple way to compensate the induced distortions. A simple algebraic formalism is proposed based on an unconventional measuring approach, which makes use of a commercial unit. Validation of the proposed compensation method for AF is conducted not only on the already studied CD Vis spectral region of a Co chiral complex with the use of an AF emulator, but also on the far UV CD spectra of poly-L-glutamic acid suspensions with different turbidity levels. Comparison with some correction factors proposed in the literature is presented.
Castiglioni
Ettore
E
JASCO Corporation, Hachioji-Shi, Tokyo, Japan. ettore.castiglioni@jasco-europe.com
Lebon
France
F
Longhi
Giovanna
G
Gangemi
Roberto
R
Abbate
Sergio
S
D002942:Circular Dichroism / Q000379:methods*; D003035:Cobalt / Q000737:chemistry; D011099:Polyglutamic Acid / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*
D011099:Polyglutamic Acid; D003035:Cobalt