Molecular analysis of CHX10 and MFRP in Chinese subjects with primary angle closure glaucoma and short axial length eyes.
OBJECTIVE
The genetic basis of primary angle closure glaucoma (PACG) has yet to be elucidated. Ocular characteristics related to PACG such as short hyperopic eyes with shallow anterior chambers suggest the involvement of genes that regulate ocular size. CHX10, a retinal homeobox gene associated with microphthalmia, and MFRP, the membrane-type frizzled-related protein gene underlying recessive nanophthalmos, represent good candidate genes for PACG due to the association with small eyes. To investigate the possible involvement of CHX10 and MFRP in PACG, we sequenced both genes in PACG patients with small ocular dimensions.


METHODS
One hundred and eight Chinese patients with axial lengths measuring 22.50 mm or less were selected for analysis. Ninety-three age- and ethnically-matched control subjects were also screened. Genomic DNA was extracted from leukocytes of peripheral blood samples, and the exons of CHX10 and MFRP were amplified by polymerase chain reaction (PCR) and subjected to bidirectional sequencing and analysis.


RESULTS
All study patients were Chinese with a mean age of 66.2+/-9.1 years (range 46-86). There were 77 females (71.3%). Forty-nine out of the one hundred and eight subjects had previous symptomatic PACG, and 59 had asymptomatic PACG. The mean axial length was 21.90+/-0.50 mm (range 19.98-22.50 mm). We identified a possible disease-causing variant in CHX10 (c.728G>A) resulting in Gly243Asp substitution in one patient. This variant was not found in 215 normal controls. Several CHX10 and MFRP polymorphisms were also identified.


CONCLUSIONS
Our results do not support a significant role for CHX10 or MFRP mutations in PACG.
Aung
Tin
T
Singapore National Eye Centre, Singapore; 2Singapore Eye Research Institute, Singapore. tin11@pacific.net.sg
Lim
Marcus C C
MC
Wong
Tina T L
TT
Thalamuthu
Anbupalam
A
Yong
Victor H K
VH
Venkataraman
Divya
D
Venkatraman
Anandalakshmi
A
Chew
Paul T K
PT
Vithana
Eranga N
EN
D000368:Aged; D000369:Aged, 80 and over; D000595:Amino Acid Sequence; D044466:Asian People / Q000235:genetics*; D001483:Base Sequence; D002681:China; D004252:DNA Mutational Analysis; D003710:Demography; D005124:Eye Abnormalities / Q000150:complications* / Q000235:genetics*; D005260:Female; D015812:Glaucoma, Angle-Closure / Q000150:complications* / Q000235:genetics*; D018398:Homeodomain Proteins / Q000737:chemistry / Q000235:genetics*; D006801:Humans; D008297:Male; D008565:Membrane Proteins / Q000737:chemistry / Q000235:genetics*; D008875:Middle Aged; D008969:Molecular Sequence Data; D009154:Mutation / Q000235:genetics; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics*; D000099274:Frizzled-Related Proteins
D018398:Homeodomain Proteins; D008565:Membrane Proteins; D014157:Transcription Factors; C490110:MFRP protein, human; D000099274:Frizzled-Related Proteins; C490289:VSX2 protein, human