Electron spin resonance in membrane research: protein-lipid interactions.
Different electron spin resonance (ESR) methods are described that allow determination of the stoichiometry and selectivity of interaction of spin-labelled lipids with integral transmembrane peptides or proteins, and also with peripheral surface-binding membrane proteins or peptides. In addition, ESR methods for determining the exchange rates of spin-labelled lipids at the protein-lipid interface are described, as well as methods to detect penetration of surface-binding peptides into the hydrophobic membrane core. Instrumental requirements are considered, and also sample handling, spin-labelling techniques and the synthesis of spin-labelled lipids.
Marsh
Derek
D
Max-Planck-Institut für biophysikalische Chemie, Abt. Spektroskopie, Am Fassberg 11, 37070 Göttingen, Germany. dmarsh@gwdg.de
D004578:Electron Spin Resonance Spectroscopy / Q000295:instrumentation / Q000379:methods*; D008055:Lipids / Q000737:chemistry; D008563:Membrane Lipids / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D008956:Models, Chemical; D013113:Spin Labels / Q000138:chemical synthesis
D008055:Lipids; D008563:Membrane Lipids; D008565:Membrane Proteins; D013113:Spin Labels