Genetic polymorphisms of IL-1A, IL-1B, IL-1RN, NFKB1, FAS, and FASL, and risk of silicosis in a Chinese occupational population.
OBJECTIVE
To test whether polymorphisms in IL-1, NF-KB, FAS, and FASL genes are associated with risk of silicosis.


METHODS
A case-control study was conducted with 183 silicosis patients and 111 silica-exposed miners who were frequency-matched by age, dust exposure duration, work location, and type of work. Genotype analysis was performed on genomic DNA, using a PCR-RFLP assay.


RESULTS
Individuals carrying the NFKB1 ins/del genotype had a decreased risk of silicosis (adjusted OR = 0.57, 95% CI = 0.32-0.998, P = 0.049) compared with subjects carrying the ins/ins genotype and individuals with the FAS-1377AA homozygote had a decreased risk of silicosis compared with those with the -1377GG genotype (adjusted OR = 0.42, 95% CI = 0.19-0.93, P = 0.03). The analysis of haplotypes of polymorphisms in FAS showed that there was a 2.71-fold (OR = 2.71, 95% CI = 1.22-6.03, P = 0.011) increased risk of silicosis for subjects with alleles of FAS-1377G and FAS-670G, compared with those carrying alleles of FAS-1377G and FAS-670A.


CONCLUSIONS
Although the polymorphisms at NFKB1, FAS-1377, and extended haplotype FAS-1377G/-670G may play a role in the development of silicosis in the Chinese population, our findings should be verified by larger studies with >1 case/control ratio.
Wu
Fen
F
Department of Occupational Health and Toxicology, School of Public Health, Fudan University, Shanghai, China.
Xia
Zhaolin
Z
Qu
Yabin
Y
Tang
Yunxia
Y
Cao
Duozhi
D
Sun
Pin
P
Christiani
David C
DC
D000368:Aged; D000369:Aged, 80 and over; D000483:Alleles; D016022:Case-Control Studies; D002681:China; D053222:Fas Ligand Protein / Q000235:genetics*; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D007375:Interleukin-1 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D008906:Mining; D052002:NF-kappa B p50 Subunit / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D018570:Risk Assessment; D012829:Silicosis / Q000235:genetics*; D019014:fas Receptor / Q000235:genetics*
C491901:FAS protein, human; D053222:Fas Ligand Protein; D007375:Interleukin-1; D052002:NF-kappa B p50 Subunit; C489548:NFKB1 protein, human; D019014:fas Receptor