Schwann cell culture from the adult animal sciatic nerve: technique and review.
An experience with the methodology of Schwann cell culture is presented using sciatic nerves from different animals (8 rats, 2 rabbits and 5 dogs). A mixed culture of the Schwann and fibroblast cells was achieved and we found some distinctions in results when using different animals, culture media (Hank's balanced salt solution and Ham's F-12 nutrient mixture with foetal calf serum), different enzymatic combinations (collagenase, trypsin and collagenase plus trypsin) and a new graded dissociation technique used for dissociation of the non-neuronal cells in these animals' sciatic nerves. The results of this work are presented with a review of the literature on the subject.
Singh
A K
AK
Department of Neurosurgery, Shinshu University School of Medicine, Matsumoto, Japan.
Shirasawa
N
N
Kyoshima
K
K
Kobayashi
S
S
Shimizu
Y
Y