Human myoblasts modulate the function of antigen-presenting cells.
Muscle biopsy specimens of myositis patients were analyzed for the presence of dendritic cells (DC) and macrophages (MPh) by immunohistochemistry. The interaction of DC and myoblasts (MB) was studied by coculture and effects on DC phenotype and function were assessed by flow cytometry and T-cell proliferation assays. Effects of MB-lysates on the phagocytic capacity of MPh were analyzed in bead-incorporation assays. Myositis specimens revealed a tendency towards more immature DC. MB modulated the maturation state of DC and DC recovered from MB-coculture had an inhibitory effect on T-cell proliferation. MB-lysates strongly stimulated MPh phagocytosis. Hypothetically, MB might modulate APC, counterbalancing immune-mediated damage.
Schwab
Nicholas
N
Department of Neurology, University of Wuerzburg, Germany.
Waschbisch
Anne
A
Wrobel
Barbara
B
Lochmüller
Hanns
H
Sommer
Claudia
C
Wiendl
Heinz
H
D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003713:Dendritic Cells / Q000378:metabolism*; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D032448:Myoblasts, Skeletal / Q000473:pathology / Q000502:physiology*; D009220:Myositis / Q000473:pathology / Q000503:physiopathology; D011956:Receptors, Cell Surface / Q000378:metabolism; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
D018528:ATP-Binding Cassette Transporters; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; D037181:Lectins, C-Type; D051907:Lysosomal Membrane Proteins; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C435500:ABCC11 protein, human