Label-free biosensor by protein grating coupler on planar optical waveguides.
We report a diffraction-based, label-free biosensor on the surface of a planar optical waveguide. By selectively immobilizing proteins on an optical waveguide, the proteins can form the ridge/valley of a latent image optical grating. Binding events with patterned surface proteins dynamically increase grating height and cause an increase in light coupling out of the waveguide. We demonstrate the efficacy of this strategy for protein detection and present the design and fabrication of a device.
Lai
Zhian
Z
Department of Electrical Engineering and Computer Science, University of California, Irvine, California 92697, USA. zlai@uci.edu
Wang
Yuli
Y
Allbritton
Nancy
N
Li
G-P
GP
Bachman
Mark
M
D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D002417:Cattle; D007074:Immunoglobulin G / Q000378:metabolism; D007834:Lasers; D055095:Optics and Photonics / Q000295:instrumentation; D011485:Protein Binding; D012031:Refractometry / Q000295:instrumentation / Q000379:methods; D012710:Serum Albumin, Bovine / Q000378:metabolism; D013499:Surface Properties
D007074:Immunoglobulin G; D012710:Serum Albumin, Bovine