[Expression of nuclear factor-kappa-B/P65 and fragile histidine triad in colorectal carcinoma and clinical significance thereof].
OBJECTIVE
To investigate the expression of nuclear factor-kappa-B (NF-KB)/P65 and fragile histidine triad (FHIT) in colorectal carcinoma and clinical significance thereof.


METHODS
Immunohistochemistry (PV6000 method) was used to detect the expression of NF-kappaB/P65 and FHIT in 359 samples of colorectal carcinoma and 35 samples of normal mucosa at least 5 cm away from the carcinoma tissues. 198 of the patients were successfully followed up by telephone interview.


RESULTS
The positive expression rates of NF-kappaB/P65 and FHIT in the colorectal carcinoma were 67.7% (243/359) and 46.0% (165/359) respectively. NF-kappaB/P65 and FHIT were all related to the clinical stage, lymphatic invasion, and distant metastasis (all P < 0.05). The expression of FHIT was negatively correlated with invasion and differentiation degree (r = -0.285 and -0. 254 respectively, both P < 0.05). The 5-year survival rate of the patients with NF-kappaB/P65 expression was significantly lower than that of the patients negative in the NF-kappaB/P65 expression while the 5-year survival rate of the patients positive in FHIT expression was significantly higher than those negative in the FHIT expression (X2 = 23.25 and 51.13 respectively, both P < 0. 05). NF-kappaB/P65 expression in colorectal carcinoma was negatively correlated with the FHIT expression (r = -0.167, P = 0.019). The expression rate of NF-kappaB/P65 in carcinoma was significantly higher, but the expression rate of FHIT in carcinoma was significantly lower than those in normal mucosa tissues (X2 = 40.78 and 15.93 respectively, both P < 0.05). NF-kappaB/P65 was an independent prognostic indicator for disease-free survival and overall survival.


CONCLUSIONS
NF-KB/P65 and FHIT play significant roles in the generation and evolution of colorectal carcinoma and may be used as useful indicators for clinical assessment of tumor biological behavior and prognosis in patients with colorectal carcinoma.
Lü
Yang
Y
Department of Pathology, General Hospital of Chinese People's Liberation Army, Beijing 100853, China.
Liu
Lin
L
Zhao
Po
P
D017766:Acid Anhydride Hydrolases / Q000096:biosynthesis*; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000473:pathology*; D005500:Follow-Up Studies; D006801:Humans; D007150:Immunohistochemistry; D008207:Lymphatic Metastasis; D009363:Neoplasm Proteins / Q000096:biosynthesis*; D009367:Neoplasm Staging; D011379:Prognosis; D016019:Survival Analysis; D051996:Transcription Factor RelA / Q000096:biosynthesis*; D000099170:Bis(5'-adenosyl)triphosphatase
D017766:Acid Anhydride Hydrolases; D009363:Neoplasm Proteins; D051996:Transcription Factor RelA; D000099170:Bis(5'-adenosyl)triphosphatase