Magnetic bead-based DNA detection with multi-layers quantum dots labeling for rapid detection of Escherichia coli O157:H7.
This work demonstrated the feasibility of detecting 250zM Escherichia coli O157:H7 eaeA target DNA by using a magnetic bead-based DNA detection assay with designed labeling strategy within 40-60min. The magnetic beads were used as the solid support for the binding probe and isolated the target DNA from the sample. The detection signals could be amplified from the multi-layers biotin-streptavidin conjugated quantum dots based on binding with specific designed biotinlyted linker. This assay method would provide a simple, rapid, and ultra-sensitive detection method for DNA or other biomolecular analysis.
Liu
Yi-Ju
YJ
Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu 30013, Taiwan.
Yao
Da-Jeng
DJ
Chang
Hwan-You
HY
Liu
Chien-Ming
CM
Chen
Chih
C
D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D019453:Escherichia coli O157 / Q000235:genetics* / Q000302:isolation & purification*; D008280:Magnetics / Q000295:instrumentation*; D045663:Quantum Dots; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013050:Spectrometry, Fluorescence / Q000295:instrumentation*; D013194:Staining and Labeling / Q000379:methods
D004269:DNA, Bacterial