Effect of gamma irradiation on lymphocyte proliferation and IL-8 production by lymphocytes isolated from platelet concentrates.
BACKGROUND
Gamma-irradiation of platelet concentrates may inactive contaminated lymphocytes and subsequently inhibit the synthesis of cytokine in platelet during storage. The aim of this study was to determine the effect of gamma-irradiation on the production of IL-8 and lymphocyte proliferation isolated from random donor platelet concentrates during 3-day storage.


METHODS
In this study, we evaluated the effect of gamma-irradiation on both lymphocyte proliferative response and IL-8 production for 29 random donor platelet concentrates (RD-PCs). All PCs were prepared from single RD by platelet-rich plasma (PRP) method were divided in two groups: 1) non-irradiated RD-PCs (n = 13) and 2) irradiated RD-PCs (n = 16). The RD-PCs were treated by gamma-irradiation (30 Gy) on days 0 and 3.


RESULTS
IL-8 increased in both groups after 3 days. It showed increase in concentration of IL-8 in irradiated and non-irradiated PCs during storage. Results of lymphocyte transformation test (LTT) indicated that the proliferation activity of lymphocyte was inhibited by gamma radiation.


CONCLUSIONS
These data indicate that gamma irradiation inhibits proliferation of lymphocyte but does not inhibit production and accumulation of IL-8 during the storage of PCs.
Pourfathollah
Ali-Akber
AA
Department of Immunology, Tarbiat Modares University and Royan Institute, Tehran, Iran.
Shaiegan
Mojgan
M
Namiri
Mehrnaz
M
Babae
Gholam-Reza
GR
D001792:Blood Platelets / Q000166:cytology*; D049109:Cell Proliferation / Q000528:radiation effects*; D005720:Gamma Rays; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis*; D008214:Lymphocytes / Q000166:cytology / Q000378:metabolism / Q000528:radiation effects*
D016209:Interleukin-8