Formyl-peptide receptor like 1: a potent mediator of the Ca2+ release-activated Ca2+ current ICRAC.
In electrically non-excitable cells, one major source of Ca(2+) influx is through the store-operated (or Ca(2+) release-activated Ca(2+)) channel by which the process of emptying the intracellular Ca(2+) stores results in the activation of Ca(2+) channels in the plasma membrane. Using both whole-cell patch-clamp and Ca(2+) imaging technique, we describe the electrophysiology mechanism underlying formyl-peptide receptor like 1 (FPRL1) linked to intracellular Ca(2+) mobilization. The FPRL1 agonists induced Ca(2+) release from the endoplasmic reticulum and subsequently evoked I(CRAC)-like currents displaying fast inactivation in K562 erythroleukemia cells which expresses FPRL1, but had almost no effect in K562 cells treated with FPRL1 RNA-interference and HEK293 cells which showed no FPRL1 expression. The currents were impaired after either complete store depletion by the sarco/endoplasmic reticulum Ca(2+)-ATPase inhibitor thapsigargin, or after inhibition of PLC by U73122. Our results present the first evidence that FPRL1 is a potent mediator in the activation of CRAC channels.
Li
Yong-Sheng
YS
Department of Pathophysiology, Tongji Medical College, Huazhong University of Science & Technology, 13 Hangkong Road, Wuhan 430030, China.
Wu
Ping
P
Zhou
Xiao-Yan
XY
Chen
Jian-Guo
JG
Cai
Lei
L
Wang
Fang
F
Xu
Lei-Ming
LM
Zhang
Xiao-Ling
XL
Chen
Ying
Y
Liu
Song-Jun
SJ
Huang
Yin-Ping
YP
Ye
Du-Yun
DY
D017302:Annexins / Q000378:metabolism; D002118:Calcium / Q000378:metabolism*; D015220:Calcium Channels / Q000737:chemistry* / Q000378:metabolism; D002460:Cell Line; D003600:Cytosol / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D020014:K562 Cells; D044045:Lipoxins / Q000378:metabolism; D008954:Models, Biological; D018408:Patch-Clamp Techniques; D044042:Receptors, Formyl Peptide / Q000378:metabolism*; D044067:Receptors, Lipoxin / Q000378:metabolism*; D012964:Sodium / Q000378:metabolism; D019284:Thapsigargin / Q000494:pharmacology
D017302:Annexins; D015220:Calcium Channels; C105101:FPR2 protein, human; D044045:Lipoxins; D044042:Receptors, Formyl Peptide; D044067:Receptors, Lipoxin; C040527:lipoxin A4; D019284:Thapsigargin; D012964:Sodium; D002118:Calcium