[Obtaining of the mutant Yarrowia lipolytica strains producing citric acid from glucose].
The possibility of obtaining mutant yeasts Yarrowia lipolytica VKM Y-2373 with increased ability to synthesize citric acid from glucose by using UV irradiation and N-methyl-NT-nitro-N-nitrosoguanidine was studied. Of 1500 colonies of the Y. lipolytica treated with either UV or N-methyl-NT-nitro-N-nitrosoguanidine, three mutants were selected that displayed higher (by 23%) biosynthetic ability as compared with the initial strain. Additionally, three mutants were selected from 1000 colonies of the Y. lipolytica exposed to a combined action of UV and N-methyl-NT-nitro-N-nitrosoguanidine; their biosynthetic activity exceeded that of the initial strain by 43.9%. The selective media with citrate and acetate were developed for a rapid selection of mutants as well as the express methods for the detection of active citric acid producers on the solid media with chalk and bromocresol containing a limiting concentration of amine nitrogen and an excess of glucose.
Finogenova
T V
TV
Puntus
I F
IF
Kamzolova
S V
SV
Lunina
Iu N
IuN
Monastyrskaia
S E
SE
Morgunov
I G
IG
Boronin
A M
AM
D000085:Acetates / Q000378:metabolism; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D014466:Ultraviolet Rays; D025062:Yarrowia / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
D000085:Acetates; D002951:Citrates; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D019343:Citric Acid; D005947:Glucose