[Effects of siRNA targeting NF-kappaBp65 against in proliferation of Hep-2 cells and the expression of mRNA and protein of NF-kappaBp65].
OBJECTIVE
To study siRNA inhibited the expression of mRNA and protein of NF-kappaBp65 in the Hep -2 cell line.


METHODS
Hep-2 were transfected with p65SiRNA. Western blotting was used to examine the protein levels of NF-kappaBp65. RT-PCR method was adopted to determine the mRNA expression of NF-kappaBp65. MTT method was adopted to investigate the proliferation of the Hep-2 cells after the transfection of p65siRNA.


RESULTS
The western blotting result showed that the level of NF-kappaBp65 protein was gradually declined after transfection of p65siRNA. The RT-PCR result showed that transfection with p65siRNA caused special degradation of the p65mRNA in Hep-2 cells at 24, 48 and 72 h. After transfection with p65siRNA.


CONCLUSIONS
p65siRNA has significant inhibition effects on the proliferation of the Hep-2 cells and expression of purpose gene mRNA and protein. The inhibition effects are time depended.
Jin
Hongjun
H
Department of Otorhinolaryngology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China.
Lou
Weihua
W
Sang
Jianzhong
J
Zhang
Yamin
Y
D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering; D051996:Transcription Factor RelA / Q000235:genetics*
C498143:RELA protein, human; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051996:Transcription Factor RelA