Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein-interacting proteins.
RNF43 is an oncogenic RING finger protein overexpressed in colorectal cancer. To dissect its biological functions, we explored RNF43-interacting proteins by pull-down assay and MS. We identified a heterodimer, p54nrb and PSF, as RNF43's binding partners and confirmed their physical interaction in vivo by the co-immunoprecipitation experiment. Immunofluorescence analysis revealed that co-expression of PSF relocates RNF43 from the nuclear periphery to the nucleoplasm. Thus, proteomic identification of RNF43-associated proteins sheds light on its dynamic interaction network in nuclear events.
Miyamoto
Kentaro
K
Discovery Research Laboratory, Tokyo R&D Center, Daiichi Pharmaceutical Co., Ltd., Daiichi-Sankyo group, Tokyo, Japan.
Sakurai
Hidetaka
H
Sugiura
Takeyuki
T
D021581:Active Transport, Cell Nucleus; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D002467:Cell Nucleus / Q000378:metabolism; D002522:Chlorocebus aethiops; D004268:DNA-Binding Proteins / Q000378:metabolism*; D019281:Dimerization; D006367:HeLa Cells; D006801:Humans; D034521:Nuclear Matrix-Associated Proteins / Q000378:metabolism*; D050810:Octamer Transcription Factors / Q000378:metabolism*; D015513:Oncogene Proteins / Q000378:metabolism*; D000072670:PTB-Associated Splicing Factor; D016601:RNA-Binding Proteins / Q000378:metabolism*; D044767:Ubiquitin-Protein Ligases
D004268:DNA-Binding Proteins; C083214:NONO protein, human; D034521:Nuclear Matrix-Associated Proteins; D050810:Octamer Transcription Factors; D015513:Oncogene Proteins; D000072670:PTB-Associated Splicing Factor; D016601:RNA-Binding Proteins; C496945:RNF43 protein, human; D044767:Ubiquitin-Protein Ligases