Bioremediation potential of formaldehyde by the marine microalga Nannochloropsis oculata ST-3 strain.
The present work is intended to investigate biodegradation of formaldehyde by the marine microalga Nannochloropsis oculata ST-3 strain. Formaldehyde concentration in the medium decreased with the growth of the ST-3 strain. It is observed that the degradation of formaldehyde concentration depends on the increased cell number of the ST-3 strain. The ST-3 strain which was adapted to formaldehyde stepwise was able to tolerate to 19.9 ppm formaldehyde and degrade 99.3% of it in the medium for 22 days. Tolerance and degradation ability of formaldehyde by the ST-3 strain was improved by stepwise increasing of the formaldehyde concentration. Transformation of [13C]formaldehyde in the medium with the passage of incubation was monitored by using a nuclear magnetic resonance (NMR) spectrometer. Formaldehyde was transformed into formate, and these two substances degraded in the medium with the passage of incubation as clearly shown by the NMR spectrum.
Yoshida
Kosuke
K
School of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu-ku, Shizuoka city, Shizuoka 424-8610, Japan. 6ated004@scc.u-tokai.ac.jp
Ishii
Hiroshi
H
Ishihara
Yoshimi
Y
Saito
Hiroshi
H
Okada
Yoshihiro
Y
D001673:Biodegradation, Environmental / Q000187:drug effects; D002452:Cell Count; D003470:Culture Media; D056890:Eukaryota / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005557:Formaldehyde / Q000378:metabolism* / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D012623:Seawater
D003470:Culture Media; D005557:Formaldehyde