Application of RP-TLC technique for the determination of dissociation constants of 1-substituted pyrrolidin-2-one derivatives.
The procedures for measuring dissociation constants (pK(a)) of twenty 1-substituted pyrrolidin-2-one derivatives are described. The dissociation constants of the compounds tested were determined using potentiometric titration, reversed-phase thin-layer chromatography (RP-TLC) and calculated using Pallas and Marvin programs. It was found that the RP-TLC method of determination of pK(a) could be considered as a feasible alternative to potentiometric titration. The Marvin program is a better tool for preliminary estimation of dissociation constant than the Pallas one.
Kulig
Katarzyna
K
Departament of Physicochemical Drug Analysis, Chair of Pharmaceutical Chemistry, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, PL-30-688 Kraków, Poland. mfkkulig@cyf-kr.edu.pl
Rybicka
Karolina
K
Malawska
Barbara
B
D002855:Chromatography, Thin Layer / Q000379:methods*; D011759:Pyrrolidines / Q000737:chemistry*
D011759:Pyrrolidines