Imaging Drosophila pupal wing morphogenesis.
Drosophila pupal (P) wing development entails a series of dynamic developmental events, such as epithelial and glial morphogenesis, that are of outstanding interest to cell biologists. Here, we first describe how to prepare P and prepupal (PP) wings for immunofluorescence microscopy. This protocol has been optimized to visualize wing epithelial architecture, such as polarized cortical domains of planar cell polarity proteins. We then provide a protocol to prepare pupae for whole mount live imaging of P wings. This procedure has allowed us to live-image glial cell migration and proliferation along wing sensory nerves.
Classen
Anne-Kathrin
AK
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Aigouy
Benoit
B
Giangrande
Angela
A
Eaton
Suzanne
S
D000818:Animals; D019521:Body Patterning; D002465:Cell Movement; D016764:Cell Polarity; D015509:Developmental Biology / Q000379:methods*; D004331:Drosophila melanogaster / Q000502:physiology*; D004847:Epithelial Cells / Q000166:cytology; D017344:Genes, Insect; D008675:Metamorphosis, Biological; D008856:Microscopy, Fluorescence / Q000295:instrumentation / Q000379:methods*; D009024:Morphogenesis; D009457:Neuroglia / Q000166:cytology; D009475:Neurons, Afferent / Q000378:metabolism; D011679:Pupa / Q000378:metabolism; D014921:Wings, Animal / Q000196:embryology