Screening and analyzing the potential bioactive components from Shaofu Zhuyu decoction, using human umbilical vein endothelial cell extraction and high-performance liquid chromatography coupled with mass spectrometry.
In this paper, a useful method for screening and analyzing the potential bioactive components in bioassay-guided fraction (SF-11) from Shaofu Zhuyu decoction was developed using human umbilical vein endothelial cell (HUVEC) extraction and high-performance liquid chromatography coupled with Q-TOF/MS spectrometry. In addition, the protective effects on HUVEC damage induced by adrenaline in vitro were also investigated. The results showed that SF-11 significantly inhibited the endothelin (ET) release and reversed the NO secretion of HUVEC (p < 0.05), and promoted the PGI(2) release of HUVEC (p < 0.05). Two effective components, paeoniflorin and typhaneoside, from SF-11 were screened and identified using live cell extract and HPLC coupled with Q-TOF/MS spectrometry. The compounds, paeoniflorin and typhaneoside, showed significantly inhibiting effects on the ET release and reversing of NO secretion of HUVEC (p < 0.05), with similar effects to SF-11, and promoting the PGI(2) release of HUVEC at the concentration of 0.208 and 0.013 micromol/mL, respectively (p < 0.05). These data indicated that the method of live cell extraction coupled with HPLC-MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCMs.
Su
Shu-Lan
SL
Jiangsu Key laboratory for TCM Formulae Research, Nanjing University of Chinese Medicine, Nanjing 210046, People's Republic of China.
Yu
Li
L
Hua
Yong-Qing
YQ
Duan
Jin-Ao
JA
Deng
Hai-Shan
HS
Tang
Yu-Ping
YP
Lu
Yin
Y
Ding
An-Wei
AW
D001565:Benzoates / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001952:Bridged-Ring Compounds / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016207:Cytokines / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D016232:Endothelins / Q000378:metabolism; D005960:Glucosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D008516:Medicine, Chinese Traditional; D015394:Molecular Structure; D039821:Monoterpenes; D015203:Reproducibility of Results; D014471:Umbilical Veins / Q000166:cytology
D001565:Benzoates; D001952:Bridged-Ring Compounds; D016207:Cytokines; D004365:Drugs, Chinese Herbal; D016232:Endothelins; D005960:Glucosides; D006027:Glycosides; D039821:Monoterpenes; C051428:typhaneoside; C015423:peoniflorin