title: Hydroxyapatite-coated polycaprolacton wide mesh as a model of open structure for bone regeneration.
abstract: In principle, three-dimensional (3D) osteoconductive grafts with a proper chemical composition, high total porosity, and fully interconnected pores are suitable carriers to provide a proper substrate for in vivo neobone tissue ingrowth. However, most porous materials carry some intrinsic limits because of their internal structure (i.e., limited macroporosity and small pore interconnection size), representing a physical constraint for a massive blood afflux and bone ingrowth and therefore for generating effective osteopermissive grafts. We therefore hypothesized that an unconventional scaffold, based on an "open-structure" concept, should not pose any limit to vascularization of grafts and consequently to the amount of bone growth. Starting from this hypothesis, we have designed and developed a 3D osteoconductive polymeric-based wide-net mesh. Polymer fibers, joining hydroxyapatite beads, were coated with a thin layer of calcium phosphate (Ca-P), coupling the osteoconductivity properties of Ca-P with the handness and bulk properties of polymers. This completely open 3D scaffold prototype was tested both in vitro and in vivo, displaying a promising in vivo blood vessel invasion and bone-forming efficiency.
authors.affiliation: Department of Informatics, Systemistics and Telematics (DIST), University of Genoa, Genoa, Italy. silvia.scaglione@unige.it
authors.forename: Silvia
authors.initials: S
authors.lastname: Scaglione
authors.forename: Cristina
authors.initials: C
authors.lastname: Ilengo
authors.forename: Marco
authors.initials: M
authors.lastname: Fato
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Quarto
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000166:cytology; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry; D002130:Calcium Phosphates / Q000737:chemistry; D002448:Cell Adhesion; D002478:Cells, Cultured; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D017886:Durapatite / Q000737:chemistry*; D004577:Electron Probe Microanalysis; D051379:Mice; D016513:Mice, SCID; D018919:Neovascularization, Physiologic; D011091:Polyesters / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D034561:Sheep, Domestic; D013234:Stem Cells / Q000166:cytology / Q000648:ultrastructure; D054457:Tissue Scaffolds / Q000737:chemistry; D014183:Transplantation, Heterologous
chemical_list: D018786:Bone Substitutes; D002130:Calcium Phosphates; D020099:Coated Materials, Biocompatible; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D017886:Durapatite; C020243:calcium phosphate