[RNA isolation from the ribonucleoproteins fixed with formaldehyde].
A method for isolating RNA from the polyribosomes and informosomes fixed with formaldehyde was developed. The ribonucleoproteins were obtained by centrifugation in CsCI density gradient. It has been demonstrated that this method makes it possible to obtain full-sized rRNA and mRNA appropriate for molecular hybridization. We succeeded in amplifying 150-nucleotide sequences of individual mRNA and demonstrating the applicability of these RNA preparations for synthesis of labeled probes for RNA arrays. The method proposed is recommended for the search for untranslated mRNA and the study of the changes in translation of individual proteins during early ontogenesis and various pathologies.
Voronina
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Pshennikova
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D000818:Animals; D003432:Cross-Linking Reagents / Q000737:chemistry; D004625:Embryo, Nonmammalian / Q000737:chemistry; D005557:Formaldehyde / Q000737:chemistry*; D009698:Nucleoproteins / Q000737:chemistry*; D011132:Polyribosomes / Q000737:chemistry; D012333:RNA, Messenger / Q000737:chemistry / Q000302:isolation & purification*; D012335:RNA, Ribosomal / Q000302:isolation & purification*; D012261:Ribonucleoproteins / Q000737:chemistry*; D014982:Xenopus laevis
D003432:Cross-Linking Reagents; D009698:Nucleoproteins; D012333:RNA, Messenger; D012335:RNA, Ribosomal; D012261:Ribonucleoproteins; D005557:Formaldehyde