title: Polymerase read-through at the first transcription termination site contributes to regulation of borna disease virus gene expression.
abstract: An unusually long noncoding sequence is located between the N gene of Borna disease virus (BDV) and the genes for regulatory factor X and polymerase cofactor P. Most of these nucleotides are transcribed and seem to control translation of the bicistronic X/P mRNA. We report here that Vero cells persistently infected with mutant viruses containing minor alterations in this control region showed almost normal levels of N, X, and P proteins but exhibited greatly reduced levels of mRNAs coding for these viral gene products. Surprisingly, cells infected with these BDV mutants accumulated a viral transcript 1.9 kb in length that represents a capped and polyadenylated mRNA containing the coding regions of the N, X, and P genes. Cells infected with wild-type BDV also contained substantial amounts of this read-through mRNA, which yielded both N and P protein when translated in vitro. Viruses carrying mutations that promoted read-through transcription at the first gene junction failed to replicate in the brain of adult rats. In the brains of newborn rats, these mutant viruses were able to replicate after acquiring second-site mutations in or near the termination signal located downstream of the N gene. Thus, sequence elements adjacent to the core termination signal seem to regulate the frequency by which the polymerase terminates transcription after the N gene. We conclude from these observations that BDV uses read-through transcription for fine-tuning the expression of the N, X, and P genes which, in turn, influence viral polymerase activity.
authors.affiliation: Department of Virology, University of Freiburg, Hermann-Herder-Strasse 11, D-79104 Freiburg, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Poenisch
authors.forename: Sandra
authors.initials: S
authors.lastname: Wille
authors.forename: Peter
authors.initials: P
authors.lastname: Staeheli
authors.forename: Urs
authors.initials: U
authors.lastname: Schneider
mesh_terms: D000818:Animals; D001483:Base Sequence; D001891:Borna disease virus / Q000235:genetics* / Q000378:metabolism; D001921:Brain / Q000378:metabolism; D002522:Chlorocebus aethiops; D015967:Gene Expression Regulation, Viral; D008957:Models, Genetic; D008969:Molecular Sequence Data; D009154:Mutation; D026723:Polyadenylation; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats; D014158:Transcription, Genetic; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D012333:RNA, Messenger