Loss of peroxisome function triggers necrosis.
Disturbance of peroxisome function can lead to various degenerative diseases during ageing. Here, we show that in yeast deletion of PEX6, encoding a protein involved in a key step of peroxisomal protein import, results in an increased accumulation of reactive oxygen species and an enhanced loss of viability upon acetic acid treatment and during early stationary phase. Cell death of ageing-like yeast cells lacking PEX6 does not depend on the apoptotic key players Yca1p and Aif1p, but instead shows markers of necrosis. Thus, we conclude that loss of peroxisomal function leads to a form of necrotic cell death.
Jungwirth
Helmut
H
Institute of Molecular Biosciences (IMB), University of Graz, Austria.
Ring
Julia
J
Mayer
Tanja
T
Schauer
Alexandra
A
Büttner
Sabrina
S
Eisenberg
Tobias
T
Carmona-Gutierrez
Didac
D
Kuchler
Karl
K
Madeo
Frank
F
D000074183:ATPases Associated with Diverse Cellular Activities; D019342:Acetic Acid / Q000494:pharmacology; D000251:Adenosine Triphosphatases / Q000235:genetics / Q000502:physiology*; D017209:Apoptosis / Q000235:genetics; D020169:Caspases / Q000378:metabolism; D017353:Gene Deletion; D009247:NADH, NADPH Oxidoreductases / Q000378:metabolism; D009336:Necrosis / Q000235:genetics*; D020675:Peroxisomes / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D012441:Saccharomyces cerevisiae / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
D029701:Saccharomyces cerevisiae Proteins; C443623:AIF1 protein, S cerevisiae; D009247:NADH, NADPH Oxidoreductases; D020169:Caspases; C456272:MCA1 protein, S cerevisiae; D000251:Adenosine Triphosphatases; D000074183:ATPases Associated with Diverse Cellular Activities; C490760:PEX6 protein, S cerevisiae; D019342:Acetic Acid