Outbreak of rotavirus variant P[8] in Seoul, South Korea.
An epidemiologic study was performed to determine the genetic variability of rotaviruses in Seoul, South Korea. In 3,174 stool specimens from children with acute diarrhea at five referral hospitals, 571 (18%) possessed the antigen of group A rotavirus detectable by ELISA--10.8% in 2004 and 28.1% in 2005. VP7 genotyping revealed that the G3 type was found in 25.6% of all typed isolates, G4 in 23.8%, G2 in 21.6%, and G1 in 17.6%. VP4 genotyping showed that the P[8] type was detected in 66.7%, P[6] in 15.6%, P[4] in 13.0%, and P[9] in 0.2%. Because the variant P[8] type could not be amplified initially by conventional P typing primers (1T-1), PCR were performed using newly designed 1T-1S primer, which revealed that 307 specimens were the variant P[8] type. Uncommon combinations such as G4P[6] and G2P[8] were also found with relatively high prevalence, 14.6% and 12.8%, respectively. Variant P[8] types were associated with an outbreak of rotavirus in 2005.
Lee
Jae-In
JI
Seoul Metropolitan Government Research Institute of Public Health & Environment, Seoul, Korea. shaoma@seoul.go.kr
Song
Mi-Ok
MO
Chung
Ju-Young
JY
Han
Tae-Hee
TH
Ahn
Young-Min
YM
Seo
Jeong-Wan
JW
Kim
Moo-Sang
MS
Kim
Min-Young
MY
Kim
Won-Yong
WY
Lee
Chan-Hee
CH
D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D007723:Korea / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*
D000956:Antigens, Viral; D017931:DNA Primers; D012367:RNA, Viral