Diagnosis of polyomavirus infection in seedcrackers (Pyrenestes sp.) and blue bills (Spermophaga haematina) using DNA in situ hybridization.
Avian polyomavirus (APV) infection was diagnosed in a closed research colony of seedcrackers (Pyrenestes sp.) and blue-bills (Spermophaga haematina) using DNA in situ hybridization. The DNA probe was a 1-kbp double-stranded PCR-generated probe that recognized a conserved nucleotide sequence within the VP-1 gene. Using this technique, APV infection was diagnosed, in 25 of 45 birds based upon examination of formalin-fixed paraffin embedded tissues. Birds infected with APV apparently had a higher incidence of hepatic necrosis, hepatitis, bacterial infections and parasitism than did birds without APV.
Garcia
A
A
Departments of Veterinary Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
Latimer
K S
KS
Niagro
F D
FD
Norton
T M
TM
Campagnoli
R P
RP
Harmon
B G
BG
Howerth
E W
EW
Ritchie
B W
BW