title: Epitope mapping of human Fas using peptide phage display.
abstract: Sandwich EIA for measurement of soluble Fas was developed on the basis of SA-7 and SA-8 monoclonal antibodies to full-length human Fas. The threshold sensitivity of the test system is 0.3 ng/ml. Several isoforms of soluble Fas were identified. The structure of SA-7 and SA-8 antibody epitopes was determined using the peptide phage library. It was shown that SA-7 antibody epitope is determined by amino acid residues 129-134 (CKPNFF), while SA-8 antibody epitope is determined by amino acid residues 94-99 (KAHFSS) of full-length Fas. Hence, sandwich EIA on the basis of SA-7 and SA-8 monoclonal antibodies for detection of soluble Fas in human serum is to detect the following Fas isoforms: FasExo6Del, FasExo4Del, FasExo4,6Del, FasExo4.7Del, and FasExo8Del.
authors.affiliation: Affiliated Department of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Organic Biochemistry, Russian Academy of Sciences, Moscow. svetlanaabbasova@rambler.ru
authors.forename: S G
authors.initials: SG
authors.lastname: Abbasova
authors.forename: Zh M
authors.initials: ZhM
authors.lastname: Shcheprova
authors.forename: A G
authors.initials: AG
authors.lastname: Laman
authors.forename: A O
authors.initials: AO
authors.lastname: Shepelyakovskaya
authors.forename: L K
authors.initials: LK
authors.lastname: Baidakova
authors.forename: I L
authors.initials: IL
authors.lastname: Rodionov
authors.forename: N E
authors.initials: NE
authors.lastname: Kushlinskii
mesh_terms: D018604:Epitope Mapping / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D019151:Peptide Library; D019014:fas Receptor / Q000276:immunology*
chemical_list: D019151:Peptide Library; D019014:fas Receptor