title: Gene expression profiles of adipose tissue of obese rats after central administration of neuropeptide Y-Y5 receptor antisense oligodeoxynucleotides by cDNA microarrays.
abstract: To investigate the gene expression profiles of adipose tissue of obese rats after central administration of neuropeptide Y-Y5 receptor antisense oligodeoxynucleotides (ODNs), Y5 receptor antisense, mismatched ODNs or vehicle was intracerebroventricularly injected and cDNA microarrays were undertaken. Central administration of NPY-Y5 receptor antisense ODNs decreased food intake, body weight and serum insulin compared with both vehicle and mismatched ODNs. The average area of adipocytes both at retroperitoneal and epididymal adipose tissue were fall in antisense group while only the weight of the retroperitoneal fat pats was reduced in antisense group. cDNA microarrays containing 18,000 genes/Ests were used to investigate gene expression of adipose tissue. Autoradiographic analysis showed that 404, 81, and 34 genes were differently expressed over twofold, threefold, and fivefold, respectively. The analysis of gene expression profiles indicated that 332 genes were up-regulated and 187 genes were down-regulated in response to Y5 receptor antisense ODNs treatment. Different clusters of genes associated with apoptosis, signal transduction, energy metabolism, lipid metabolism, etc., such as FXR1, PHLDA1, MAEA, PIK3R1, ICAM2, PITPN, CALM2, CAMK2D, PKIA, DRD2, SLC25A14, CKB, AADAC, LIPA, ACOX3, FADS1, were concerned. Analysis of differentially expressed genes will help to understand the effects of Y5 receptor antisense ODNs therapy.
authors.affiliation: Department of Pediatrics, Nanjing Maternity and Child Health Hospital of Nanjing Medical University, 210004 Nanjing, China; Institute of Pediatrics of Nanjing Medical University, 210029 Nanjing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Qiu
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Ni
authors.forename: Rong-Hua
authors.initials: RH
authors.lastname: Chen
authors.forename: Chen-Bo
authors.initials: CB
authors.lastname: Ji
authors.forename: Feng
authors.initials: F
authors.lastname: Liu
authors.forename: Chun-Mei
authors.initials: CM
authors.lastname: Zhang
authors.forename: Chun-Lin
authors.initials: CL
authors.lastname: Gao
authors.forename: Xiao-Hui
authors.initials: XH
authors.lastname: Chen
authors.forename: Mei-Ling
authors.initials: ML
authors.lastname: Tong
authors.forename: Xia
authors.initials: X
authors.lastname: Chi
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Zhou
authors.forename: Xi-Rong
authors.initials: XR
authors.lastname: Guo
mesh_terms: D052438:Adipocytes, White / Q000187:drug effects; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004435:Eating / Q000187:drug effects; D020869:Gene Expression Profiling; D007328:Insulin / Q000097:blood; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017476:Receptors, Neuropeptide Y / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D007328:Insulin; D016376:Oligonucleotides, Antisense; D017476:Receptors, Neuropeptide Y; C100568:neuropeptide Y5 receptor