title: On-site manipulation of single chromosomal DNA molecules by using optically driven microstructures.
abstract: We report a novel method for manipulation of single giant DNA molecules under a video microscope. Using optically driven microstructures, we manipulated chromosomal DNA of length in the order of millimetres, extended by electroosmotic flow without DNA breakage in aqueous solution: we picked up DNA, using microfabricated hooks and wound it around microfabricated bobbins.
authors.affiliation: Department of Micro Engineering, Kyoto University, Kyoto, 606-8501, Japan. k.terao@kt5.ecs.kyoto-u.ac.jp
authors.forename: Kyohei
authors.initials: K
authors.lastname: Terao
authors.forename: Masao
authors.initials: M
authors.lastname: Washizu
authors.forename: Hidehiro
authors.initials: H
authors.lastname: Oana
mesh_terms: D002875:Chromosomes / Q000648:ultrastructure*; D004271:DNA, Fungal / Q000648:ultrastructure*; D008855:Microscopy, Electron, Scanning / Q000295:instrumentation* / Q000379:methods*; D012568:Schizosaccharomyces / Q000648:ultrastructure
chemical_list: D004271:DNA, Fungal