[Effects of oxymetazoline hydrochloride on ex vivo human nasal cilia movement measured with high-speed digital microscopy].
OBJECTIVE
To investigate the effects of oxymetazoline hydrochloride on ex vivo human nasal cilia movement.


METHODS
Ciliary beat frequency (CBF) of cultured human nasal epithelial cells was measured by high-speed digital microscopy in HBSS and oxymetazoline hydrochloride of different concentrations in 20 minutes. RESULTS; CBF of cultured nasal epithelial cells in HBSS and 0.25 g/L oxymetazoline hydrochloride did not show significant changes in 20 minutes (F = 0.098, P = 1.00). However, in 0.50 g/L and 1.00 g/L oxymetazoline hydrochloride, CBF increased slightly in 3 -4 minutes and reached the apex, then decreased gradually. At the end of observation, CBF showed no significant difference in 0.50 g/L, (F = 2.94, P = 0.05) but there was a significant lower CBF in 1.00 g/L. In the first 3 minutes, the CBF in 2.00 g/L oxymetazoline hydrochloride was stable, and then slowed gradually. After 16 minutes, there was significant difference. In initial, the highest normalized CBF of each group showed no significant difference. However, the lowest normalized CBF of 1.00 and 2.00 g/L oxymetazoline hydrochloride showed a significant difference with HBSS, 0.25 and 0.50 g/L oxymetazoline hydrochloride.


CONCLUSIONS
Oxymetazoline had a concentration-dependent inhibitory effect on cultured human nasal CBF from 0.25 to 2.00 g/L. The inhibitory effect increased with the concentration going up. Oxymetazoline hydrochloride of 0.50 g/L might be the optimal choice for clinical application.
Song
Xiao-Hong
XH
Department of Otorhinolaryngology Head and Neck Surgery, Affiliated Beijing Tongren Hospital of Capital Medical University, Beijing 100730, China.
Zhang
Luo
L
Han
De-Min
DM
Wang
Kui-Ji
KJ
Wang
Hong
H
Zhang
Wei
W
D002478:Cells, Cultured; D002923:Cilia / Q000187:drug effects*; D006801:Humans; D008853:Microscopy; D009079:Mucociliary Clearance / Q000187:drug effects; D009297:Nasal Mucosa / Q000187:drug effects*; D010109:Oxymetazoline / Q000494:pharmacology*; D012852:Sinusitis / Q000503:physiopathology*; D046509:Tissue Culture Techniques
D010109:Oxymetazoline