In vitro model to estimate gut inflammation using co-cultured Caco-2 and RAW264.7 cells.
A system for assessing the anti-inflammatory effect of food factors was developed by establishing a co-culture system with intestinal epithelial Caco-2 cells (apical side) and macrophage RAW264.7 cells (basolateral side). In this system, the stimulation of RAW264.7 cells with lipopolysaccharide was followed by a decrease in transepithelial electrical resistance, which is a marker of the integrity of the Caco-2 monolayer and an increase in TNF-alpha production from RAW264.7 cells and IL-8 mRNA expression in Caco-2 cells. Treatment with anti-TNF-alpha antibodies or budesonide suppressed in increase in TNF-alpha production and IL-8 mRNA expression. These results indicated that this co-culture model could imitate the gut inflammation in vivo. In addition, fucoidan, sulphated polysaccharides from brown algae, was employed as a candidate of evolution and added to the apical side of this model. Fucoidan suppressed IL-8 gene expression through a reduction in TNF-alpha production from RAW264.7 cells stimulated with lipopolysaccharide.
Tanoue
Takeshi
T
Department of Agrobioscience, Graduate School of Agricultural Science, Kobe University, 1-1, Rokkodai-cho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.
Nishitani
Yosuke
Y
Kanazawa
Kazuki
K
Hashimoto
Takashi
T
Mizuno
Masashi
M
D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology; D018938:Caco-2 Cells; D018920:Coculture Techniques; D003092:Colitis / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D016209:Interleukin-8 / Q000096:biosynthesis / Q000235:genetics; D051379:Mice; D008954:Models, Biological; D000459:Phaeophyceae / Q000737:chemistry; D011134:Polysaccharides / Q000494:pharmacology; D012333:RNA, Messenger / Q000096:biosynthesis; D013431:Sulfates / Q000494:pharmacology; D014409:Tumor Necrosis Factor-alpha / Q000037:antagonists & inhibitors / Q000096:biosynthesis / Q000235:genetics
D000894:Anti-Inflammatory Agents, Non-Steroidal; D016209:Interleukin-8; D011134:Polysaccharides; D012333:RNA, Messenger; D013431:Sulfates; D014409:Tumor Necrosis Factor-alpha; C007789:fucoidan