Duration of chronic inflammation alters gene expression in muscle from untreated girls with juvenile dermatomyositis.
BACKGROUND
To evaluate the impact of the duration of chronic inflammation on gene expression in skeletal muscle biopsies (MBx) from untreated children with juvenile dermatomyositis (JDM) and identify genes and biological processes associated with the disease progression, expression profiling data from 16 girls with active symptoms of JDM greater than or equal to 2 months were compared with 3 girls with active symptoms less than 2 months.


RESULTS
Seventy-nine genes were differentially expressed between the groups with long or short duration of untreated disease. Genes involved in immune responses and vasculature remodelling were expressed at a higher level in muscle biopsies from children with greater or equal to 2 months of symptoms, while genes involved in stress responses and protein turnover were expressed at a lower level. Among the 79 genes, expression of 9 genes showed a significant linear regression relationship with the duration of untreated disease. Five differentially expressed genes--HLA-DQA1, smooth muscle myosin heavy chain, clusterin, plexin D1 and tenomodulin--were verified by quantitative RT-PCR. The chronic inflammation of longer disease duration was also associated with increased DC-LAMP+ and BDCA2+ mature dendritic cells, identified by immunohistochemistry.


CONCLUSIONS
We conclude that chronic inflammation alters the gene expression patterns in muscle of untreated children with JDM. Symptoms lasting greater or equal to 2 months were associated with dendritic cell maturation and anti-angiogenic vascular remodelling, directly contributing to disease pathophysiology.
Chen
Yi-Wen
YW
Center for Genetic Medicine Research, Children's National Medical Center, Washington, DC, USA. ychen@cnmcresearch.org
Shi
Rongye
R
Geraci
Nicholas
N
Shrestha
Sheela
S
Gordish-Dressman
Heather
H
Pachman
Lauren M
LM
D002454:Cell Differentiation / Q000235:genetics / Q000276:immunology; D002648:Child; D002675:Child, Preschool; D002908:Chronic Disease; D003713:Dendritic Cells / Q000378:metabolism / Q000473:pathology; D003882:Dermatomyositis / Q000097:blood / Q000235:genetics* / Q000276:immunology*; D018450:Disease Progression; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000276:immunology*; D006683:HLA-DQ Antigens / Q000235:genetics* / Q000276:immunology; D006801:Humans; D018482:Muscle, Skeletal / Q000098:blood supply / Q000276:immunology / Q000378:metabolism* / Q000473:pathology; D009389:Neovascularization, Pathologic / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D011110:Polymorphism, Genetic; D012720:Severity of Illness Index; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics* / Q000276:immunology
D006683:HLA-DQ Antigens; D014409:Tumor Necrosis Factor-alpha