title: Small-interference RNA-mediated knock-down of aldehyde oxidase 1 in 3T3-L1 cells impairs adipogenesis and adiponectin release.
abstract: Aldehyde oxidase 1 (AOX1) is highly abundant in the liver and oxidizes aldehydes thereby generating reactive oxygen species. Enzymes involved in detoxification of aldehydes are expressed in adipocytes and alter adipogenesis, therefore the functional role of AOX1 in adipocytes was analyzed. AOX1 mRNA was higher in visceral compared to subcutaneous human adipose tissue but AOX1 protein was detected in both fat depots. AOX1 expression in adipocytes was confirmed by immunohistochemistry and immunoblot. AOX1 was induced during adipocytic differentiation and was downregulated by fenofibrate in differentiated cells. Knock-down of AOX1 in preadipocytes led to impaired lipid storage and adiponectin release in the differentiated cells. These data indicate that AOX1 is essential for adipogenesis and may link energy and drug metabolism.
authors.affiliation: Department of Internal Medicine I, Regensburg University Hospital, Regensburg, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Weigert
authors.forename: Markus
authors.initials: M
authors.lastname: Neumeier
authors.forename: Sabrina
authors.initials: S
authors.lastname: Bauer
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Mages
authors.forename: Andreas A
authors.initials: AA
authors.lastname: Schnitzbauer
authors.forename: Aiman
authors.initials: A
authors.lastname: Obed
authors.forename: Benedikt
authors.initials: B
authors.lastname: Gröschl
authors.forename: Arndt
authors.initials: A
authors.lastname: Hartmann
authors.forename: Andreas
authors.initials: A
authors.lastname: Schäffler
authors.forename: Charalampos
authors.initials: C
authors.lastname: Aslanidis
authors.forename: Jürgen
authors.initials: J
authors.lastname: Schölmerich
authors.forename: Christa
authors.initials: C
authors.lastname: Buechler
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000201:enzymology*; D050156:Adipogenesis / Q000187:drug effects / Q000235:genetics*; D052242:Adiponectin / Q000378:metabolism*; D000445:Aldehyde Oxidoreductases / Q000235:genetics / Q000502:physiology*; D000818:Animals; D011345:Fenofibrate / Q000494:pharmacology; D051379:Mice; D000077205:Pioglitazone; D034741:RNA, Small Interfering / Q000235:genetics; D045162:Thiazolidinediones / Q000494:pharmacology
chemical_list: D052242:Adiponectin; D034741:RNA, Small Interfering; D045162:Thiazolidinediones; D000445:Aldehyde Oxidoreductases; C443116:Aox3 protein, mouse; D011345:Fenofibrate; D000077205:Pioglitazone