Expression of Pkd2l2 in testis is implicated in spermatogenesis.
Pkd2l2 is a novel member of the polycystic kidney disease (PKD) gene family in mammals. Prominently expressed in testis, this gene is still poorly understood. In this study, reverse transcription polymerase chain reaction (RT-PCR) results showed a time-dependent expression pattern of Pkd2l2 in postnatal mouse testis. Immunohistochemical analysis revealed that Pkd2l2 encoded a protein, polycystin-L2, which was predominantly detectable in the plasma membrane of spermatocytes and round spermatids, as well as in the head and tail of elongating spermatids within seminiferous tubules in mouse testis tissue sections of postnatal day 14 and adult mice. A green fluorescent fusion protein of Pkd2l2 resided in the plasma membrane of HEK 293 and MDCK cells, suggesting that it functions as a plasma membrane protein. Overexpression of Pkd2l2 increased the intracellular calcium concentration of MDCK cells, as detected by flow cytometry. Collectively, these data indicated that Pkd2l2 may be involved in the mid-late stage of spermatogenesis through modulation of the intracellular calcium concentration.
Chen
Ye
Y
Core Facility of Gene Engineered Mice, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Sichuan, China.
Zhang
Zheng
Z
Lv
Xiao-Yan
XY
Wang
Yi-Dong
YD
Hu
Zhong-Guo
ZG
Sun
Huan
H
Tan
Rui-Zhi
RZ
Liu
Yu-Hang
YH
Bian
Guo-Hui
GH
Xiao
Yan
Y
Li
Qin-Wei
QW
Yang
Qiu-Tan
QT
Ai
Jian-Zhong
JZ
Feng
Lu
L
Yang
Yang
Y
Wei
Yu-Quan
YQ
Zhou
Qin
Q
D000818:Animals; D002118:Calcium / Q000378:metabolism; D015220:Calcium Channels; D002462:Cell Membrane / Q000378:metabolism; D018076:DNA, Complementary / Q000096:biosynthesis / Q000235:genetics; D004285:Dogs; D005434:Flow Cytometry; D049452:Green Fluorescent Proteins / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008562:Membrane Glycoproteins / Q000096:biosynthesis* / Q000235:genetics*; D051379:Mice; D008810:Mice, Inbred C57BL; D008969:Molecular Sequence Data; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012671:Seminiferous Tubules / Q000378:metabolism; D013091:Spermatogenesis / Q000235:genetics* / Q000502:physiology*; D013347:Subcellular Fractions / Q000378:metabolism; D013737:Testis / Q000254:growth & development / Q000378:metabolism* / Q000502:physiology*
D015220:Calcium Channels; D018076:DNA, Complementary; D008562:Membrane Glycoproteins; C511305:Pkd2l2 protein, mouse; D049452:Green Fluorescent Proteins; D002118:Calcium