title: [Study on isolation of Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique].
abstract: OBJECTIVE
  To isolate and purify Chlorogenic acid from Eucommia ulmoides leaves by ultrafiltration technique.
  
  
  METHODS
  Chlorogenic acid transferring ratio was investigated by temperature, pressure, time, cut-off molecular mass.
  
  
  RESULTS
  The optimal conditions for the ultrafiltration technique were finally determined. It was found that, for the membrane with a cut-off molecular mass of 20000, when the temperature, pressure, time were respectively 40 degrees C, 0.36 MPa, 20 minutes and adding 24L water, yield of Chlorogenic acid was 99.38%.
  
  
  CONCLUSIONS
  High Chlorogenic acid transfering ratio can be gotten by ultrafiltration technique, which is helpful to the further isolation and purification of Chlorogenic acid in the subsequent process.
authors.affiliation: National Engineering Research Center for Modernization of Extraction and Separation Process of TCM, Guangzhou 510240, China. yangzujin@126.com
authors.forename: Zu-Jin
authors.initials: ZJ
authors.lastname: Yang
authors.forename: Yan-Bin
authors.initials: YB
authors.lastname: Jiang
authors.forename: Fa-Huan
authors.initials: FH
authors.lastname: Ge
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Yuan
authors.forename: Shao-Chong
authors.initials: SC
authors.lastname: Yu
mesh_terms: D002726:Chlorogenic Acid / Q000737:chemistry / Q000302:isolation & purification*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification*; D031283:Eucommiaceae / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D008970:Molecular Weight; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011312:Pressure; D013678:Technology, Pharmaceutical / Q000379:methods*; D013696:Temperature; D014462:Ultrafiltration / Q000379:methods
chemical_list: D004365:Drugs, Chinese Herbal; D002726:Chlorogenic Acid