title: Clostridium perfringens necrotizing enteritis of the fowl: a light microscopic, immunohistochemical and ultrastructural study of spontaneous disease.
abstract: Moderate lesions of spontaneous necrotic enteritis were investigated by light microscopy, immunoperoxidase staining, and electron microscopy. Concurrent coccidial infection was detected in the intestinal mucosa of the majority of diseased birds. Electron microscopy of intestinal epithelium revealed vesiculation and blebbing of the luminal cell membrane, as well as degenerative changes of cytoplasmic organelles. Using immunoperoxidase staining, Clostridium perfringens was identified and shown to constitute the majority of organisms in necrotic tissues, whereas bacteria were never seen in viable tissue. Thus, Clostridium perfringens always appeared to be present in the lesions and to act locally, causing a coagulative type of necrosis. The observed vesiculation of epithelial cell membranes indicates that the hydrolysis of membrane components by bacterial toxins could be important in the pathogenesis of necrotic enteritis.
authors.affiliation: Section of Pathology, Central Veterinary Laboratory, Oslo, Norway.
authors.forename: M
authors.initials: M
authors.lastname: Kaldhusdal
authors.forename: O
authors.initials: O
authors.lastname: Evensen
authors.forename: T
authors.initials: T
authors.lastname: Landsverk