The composition of cell wall skeleton and outermost lipids of Mycobacterium vaccae is modified by ethambutol treatment.
Ethambutol (EMB) is a first line drug in tuberculosis treatment inhibiting the biosynthesis of arabinogalactan, which is a component of the mycobacterial cell wall. The growth of Mycobacterium vaccae cells in the presence of EMB increases cell wall permeability, which was monitored by beta-sitosterol biotransformation. GC/MS and GLC/MS (gas chromatography/mass spectrometry) analysis revealed dramatic changes in the content of covalently bound mycolic acids and in molar ratio galactose (Gal) to arabinose (Ara) in the cell envelopes of EMB-treated cells. The detected variations in the compositions of fatty acids indicate that both the cell wall skeleton and outer layer (free lipids) are decomposed due to EMB treatment.
Rumijowska-Galewicz
Anna
A
Institute for Medical Biology Polish Academy of Sciences, Łódź, Poland. arumijowska@cbm.pan.pl
Korycka-Machała
Małgorzata
M
Lisowska
Katarzyna
K
Dziadek
Jarosław
J
D000995:Antitubercular Agents / Q000494:pharmacology*; D001089:Arabinose / Q000378:metabolism; D002463:Cell Membrane Permeability; D017358:Cell Wall Skeleton / Q000187:drug effects / Q000378:metabolism*; D004977:Ethambutol / Q000494:pharmacology*; D005685:Galactans / Q000037:antagonists & inhibitors / Q000378:metabolism*; D005690:Galactose / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D006017:Glycolipids / Q000378:metabolism; D009161:Mycobacterium / Q000187:drug effects / Q000254:growth & development / Q000378:metabolism*; D009171:Mycolic Acids / Q000378:metabolism; D010743:Phospholipids / Q000378:metabolism; D011135:Polysaccharides, Bacterial / Q000187:drug effects / Q000378:metabolism*; D012855:Sitosterols / Q000378:metabolism
D000995:Antitubercular Agents; D017358:Cell Wall Skeleton; D005685:Galactans; D006017:Glycolipids; D009171:Mycolic Acids; D010743:Phospholipids; D011135:Polysaccharides, Bacterial; D012855:Sitosterols; C025473:gamma-sitosterol; D004977:Ethambutol; D001089:Arabinose; C005653:arabinogalactan; D005690:Galactose