[Study on quality standard of Poria cocos].
OBJECTIVE
To perfect the standard of Poria cocos (Schw.).


METHODS
TLC and HPLC fingerprint were used to identify triterpenoids from P. cocos. The content of pachymic acid and dehydropachymic acid were determined by HPLC.


RESULTS
Pachymic acid showed a good linear relationship at a range of 1.52 - 7.60 microg/ml, r = 0.9994, the average recovery was 96.90%, RSD = 1.09%. Dehydropachymic acid had a good linear relationship at a range of 0.35 - 1.75 microg/ml, r = 0.9991, the average recovery was 97.86%, RSD = 1.52%.


CONCLUSIONS
The methods are special and accurate, and can be used for the quality control of P. cocos.
Xu
Xiu-Ying
XY
College of Bioengineer, Chongqing Institute of Technology, Chongqing 400050, China. yxx651116@cqit.edu.cn
Yi
Zhong-Hong
ZH
Zheng
Yi-Min
YM
Fu
Shan-Quan
SQ
D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002855:Chromatography, Thin Layer / Q000379:methods; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards; D010598:Pharmacognosy; D055441:Poria / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D014315:Triterpenes / Q000032:analysis* / Q000302:isolation & purification
D004365:Drugs, Chinese Herbal; D014315:Triterpenes; C102487:pachymic acid