One-step preparation of antibody and oligonucleotide dual-labeled gold nanoparticle bio-probes and their properties.
A novel method of one-step preparation of dual-labeled gold nanoparticle bio-probes was established by the electrostatic adsorption and the covalent bonding of gold nanoparticles with antibodies and thiol-modified oligonucleotides, respectively. Characterization of probes, the coverage and activity of antibodies and oligonucleotides on probe surfaces were detected. The results indicated that the gold nanoparticles labeled with antibodies and oligonucleotides possess good bioactivity and the coverage of oligonucleotide and antibody on a dual-labeled gold nanoparticle bio-probe was (92 +/- 20) and (8 +/- 3), respectively. The preparative method is simple and stable. The dual-labeled gold nanoparticle bio-probes have an application value in detection of ultramicro protein.
Kong
Xiao-Li
XL
Clinical Medical Research Center, The Second Clinical Medical College (Shenzhen Peoples' Hospital), Jinan University, 1017 North Road of Eastern Gate, Shenzhen 518020, China.
Qiao
Fei-Yan
FY
Qi
Hui
H
Li
Fu-Rong
FR
D000327:Adsorption; D015374:Biosensing Techniques / Q000379:methods*; D006046:Gold; D007074:Immunoglobulin G; D007150:Immunohistochemistry; D053768:Metal Nanoparticles / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission; D015335:Molecular Probes; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D016133:Polymerase Chain Reaction; D012394:Rosaniline Dyes; D013873:Thionucleotides / Q000737:chemistry
D007074:Immunoglobulin G; D015335:Molecular Probes; D015345:Oligonucleotide Probes; D009841:Oligonucleotides; D012394:Rosaniline Dyes; D013873:Thionucleotides; D006046:Gold; C004692:coomassie Brilliant Blue