Molecular cloning of CYP1A gene and its expression by benzo(a)pyrene from goldfish (Carassius auratus).
We cloned and sequenced the cytochrome P450 1A (CYP1A) gene from goldfish (Carassius auratus). It has a 1581 bp open reading frame that encodes a 526 amino acid protein with a theoretical molecular weight of 59.02 kDa. The CYP1A amino acid sequence clusters in a monophyletic group with other fish CYP1As, and more closely related to zebrafish CYP1A (91% identity) than to other fish CYP1As. Exposure to benzo(a)pyrene (BaP) by intraperitoneal injection increased biliary BaP metabolites and liver CYP1A gene expression. BaP exposure also increased CYP1A gene expression in extrahepatic organs, including intestine, and gill, which are sensitive to aqueous and dietary exposure to Arylhydrocarbon receptor (AhR) agonists. Therefore, goldfish CYP1A identified in this study offers basic information for further research related to biomarker use of CYP1A of goldfish.
Oh
Seung-Min
SM
College of Pharmacy, Sungkyunkwan University, #300, Cheoncheondong, Jangan-Gu, Suwon, Gyeonggi-do 440-746, Korea.
Ryu
Byung Taek
BT
Kim
Ha Ryong
HR
Choi
Kyunghee
K
Chung
Kyu-Hyuck
KH
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D001564:Benzo(a)pyrene / Q000493:pharmacokinetics / Q000494:pharmacology*; D001646:Bile / Q000378:metabolism; D003001:Cloning, Molecular; D019363:Cytochrome P-450 CYP1A1 / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006054:Goldfish / Q000235:genetics* / Q000378:metabolism; D007668:Kidney / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008969:Molecular Sequence Data; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
D001564:Benzo(a)pyrene; D019363:Cytochrome P-450 CYP1A1