A Role for galectin-3 in renal tissue damage triggered by ischemia and reperfusion injury.
Ischemic-reperfusion injury (IRI) triggers an inflammatory response involving neutrophils/macrophages, lymphocytes and endothelial cells. Galectin-3 is a multi-functional lectin with a broad range of action such as promotion of neutrophil adhesion, induction of oxidative stress, mastocyte migration and degranulation, and production of pro-inflammatory cytokines. The aim of this study was evaluate the role of galectin-3 in the inflammation triggered by IRI. Galectin-3 knockout (KO) and wild type (wt) mice were subjected to 45 min of renal pedicle occlusion. Blood and kidney samples were collected at 6, 24, 48 and 120 h. Blood urea was analyzed enzymatically, while MCP-1, IL-6 and IL-1beta were studied by real-time PCR. Reactive oxygen species (ROS) was investigated by flow cytometry. Morphometric analyses were performed at 6, 24, 48 and 120 h after reperfusion. Urea peaked at 24 h, being significantly lower in knockout animals (wt = 264.4 +/- 85.21 mg/dl vs. gal-3 KO = 123.74 +/- 29.64 mg/dl, P = 0.001). Galectin-3 knockout animals presented less acute tubular necrosis and a more prominent tubular regeneration when compared with controls concurrently with lower expression of MCP-1, IL-6, IL-1beta, less macrophage infiltration and lower ROS production at early time points. Galectin-3 seems to play a role in renal IRI involving the secretion of macrophage-related chemokine, pro-inflammatory cytokines and ROS production.
Fernandes Bertocchi
Ana Paula
AP
Laboratory of Clinical and Experimental Immunology, Division of Nephrology, Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo, Brazil.
Campanhole
Gabriela
G
Wang
Pamella Huey Mei
PH
Gonçalves
Giselle Martins
GM
Damião
Márcio José
MJ
Cenedeze
Marcos Antônio
MA
Beraldo
Felipe Caetano
FC
de Paula Antunes Teixeira
Vicente
V
Dos Reis
Marlene Antônia
MA
Mazzali
Marilda
M
Pacheco-Silva
Alvaro
A
Câmara
Niels Olsen Saraiva
NO
D000818:Animals; D001324:Autoantigens; D015415:Biomarkers; D018932:Chemokine CCL2 / Q000096:biosynthesis / Q000235:genetics; D004247:DNA / Q000235:genetics*; D004195:Disease Models, Animal; D005434:Flow Cytometry; D005500:Follow-Up Studies; D037502:Galectin 3 / Q000096:biosynthesis / Q000235:genetics*; D015870:Gene Expression; D007150:Immunohistochemistry; D053583:Interleukin-1beta / Q000096:biosynthesis / Q000235:genetics; D015850:Interleukin-6 / Q000096:biosynthesis / Q000235:genetics; D007668:Kidney / Q000098:blood supply*; D016030:Kidney Transplantation / Q000473:pathology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D016133:Polymerase Chain Reaction; D017382:Reactive Oxygen Species / Q000378:metabolism; D015427:Reperfusion Injury / Q000378:metabolism* / Q000473:pathology
D001324:Autoantigens; D015415:Biomarkers; C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D037502:Galectin 3; D053583:Interleukin-1beta; D015850:Interleukin-6; D017382:Reactive Oxygen Species; D004247:DNA