title: Immunocytochemical diagnosis of Pacheco's disease.
abstract: An indirect immunoperoxidase method for the detection of herpesvirus in the liver and digestive tract of two psittacines (Ara macao and Cacatua sulphurea) with Pacheco's disease is described. The anti-Pachecovirus antiserum used was obtained from hyperimmunized specific pathogen-free chickens. It is concluded that this easily executed technique yields reliable results, allows a firm diagnosis of Pacheco's disease to be made and it would be suitable for investigating the pathogenesis of Pacheco's disease.
authors.affiliation: Histology and Pathology Unit, School of Veterinary Medicine, Universidad Autonoma de Barcelona, Bellaterra, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Ramis
authors.forename: D
authors.initials: D
authors.lastname: Fondevila
authors.forename: J
authors.initials: J
authors.lastname: Tarres
authors.forename: L
authors.initials: L
authors.lastname: Ferrer