Evaluation of tumor microsatellite instability in laryngeal cancer using five quasimonomorphic mononucleotide repeats and pentaplex PCR.
OBJECTIVE
To determine microsatellite instability (MSI) in squamous cell carcinoma of the larynx.


METHODS
The study was performed in a group of 30 male patients, aged 42-47 years. All patients underwent total laryngectomy with lymph nodectomy. Histologically all tumors were squamous cell carcinomas. Detection of MSI was based on comparison of allelic profiles generated from amplification of matching normal and tumor DNA.


RESULTS
The appearance of novel alleles in the tumor DNA indicated microsatellite instability. MSI analysis showed a microsatellite stable phenotype in 23 cases (77%).


CONCLUSIONS
MSI may not contribute to the development of squamous cell carcinoma of the larynx.
Rózańska-Kudelska
M
M
Departament of Otolaryngology, Medical University of Bialystok, Poland.
Walenczak
I
I
Pepiński
W
W
Sieśkiewicz
A
A
Skawrońska
M
M
Rogowski
M
M
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000235:genetics*; D005820:Genetic Testing / Q000379:methods*; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics*; D008297:Male; D053842:Microsatellite Instability; D008875:Middle Aged; D010641:Phenotype; D016133:Polymerase Chain Reaction / Q000379:methods*