Oxidative stress, erythrocyte ageing and plasma non-protein-bound iron in diabetic patients.
Increased oxidative stress and decreased life span of erythrocytes (RBCs) are repeatedly reported in diabetes. In the aim to elucidate the mechanism of the latter, i.e. the events leading to erythrocyte ageing, this study determined in RBCs from diabetic patients iron release in a free desferrioxamine-chelatable form (DCI), methemoglobin (MetHb) formation, binding of autologous IgG to membrane proteins and in plasma non-protein-bound iron (NPBI), F(2)-Isoprostanes (F(2)-IsoPs) and advanced oxidation protein products (AOPP). DCI and MetHb were higher in diabetic RBCs than in controls and autologous IgG binding occurred in a much higher percentage of diabetic patients than controls. A significant correlation between DCI and IgG binding was found in diabetic RBCs. Plasma NPBI, esterified F(2)-IsoPs and AOPP were higher in diabetic patients and a significant correlation was found between plasma NPBI and intra-erythrocyte DCI. The increased DCI and autologous IgG binding appear to be important factors in the accelerated removal of RBCs from the blood stream in diabetes and the increase in plasma NPBI could play an important role in the increased oxidative stress.
Leoncini
Silvia
S
Department of Pathophysiology, Experimental Medicine and Public Health, University of Siena, Siena, Italy.
Rossi
Viviana
V
Signorini
Cinzia
C
Tanganelli
Italo
I
Comporti
Mario
M
Ciccoli
Lucia
L
D000368:Aged; D016022:Case-Control Studies; D003676:Deferoxamine / Q000378:metabolism; D003922:Diabetes Mellitus, Type 1 / Q000097:blood* / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000473:pathology; D004905:Erythrocyte Aging; D004912:Erythrocytes / Q000378:metabolism* / Q000473:pathology; D028441:F2-Isoprostanes / Q000378:metabolism; D005260:Female; D006801:Humans; D007501:Iron / Q000097:blood*; D008297:Male; D008706:Methemoglobin / Q000378:metabolism; D008875:Middle Aged; D018384:Oxidative Stress; D011485:Protein Binding
D028441:F2-Isoprostanes; D008706:Methemoglobin; D007501:Iron; D003676:Deferoxamine