Rapid and reliable identification of Streptococcus anginosus group isolates to the species level by real-time PCR and melting curve analysis.
A real-time PCR assay is described for rapid and accurate identification of the Streptococcus anginosus group members. Based on melting curve analysis, the S. anginosus species isolates could be further subdivided into two subgroups, each associated with different clinically relevant ribotypes of S. anginosus.
Desar
Ingrid M E
IM
Department of Internal Medicine, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
de Boer
Maaike
M
Bens
Corina C P M
CC
Jacobs
Jan A
JA
Mouton
Johan W
JW
Dofferhoff
Anton S M
AS
Klaassen
Corné H W
CH
D016470:Bacteremia / Q000382:microbiology; D015373:Bacterial Typing Techniques; D015342:DNA Probes; D021903:DNA, Ribosomal Spacer / Q000032:analysis / Q000235:genetics; D006801:Humans; D016133:Polymerase Chain Reaction / Q000379:methods*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D015203:Reproducibility of Results; D013045:Species Specificity; D013203:Staphylococcal Infections / Q000382:microbiology; D013210:Staphylococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D013997:Time Factors; D044366:Transition Temperature
D015342:DNA Probes; D021903:DNA, Ribosomal Spacer; D012336:RNA, Ribosomal, 16S