title: [Expression of nucleostemin mRNA and protein in the esophageal squamous cell carcinoma].
abstract: OBJECTIVE
  To investigate the mRNA and protein expression of nucleostemin (NS) in human esophageal squamous cell carcinoma.
  
  
  METHODS
  The mRNA and protein expression of NS were detected in 31 mucosal atypical hyperplasia specimens, 62 esophageal squamous cell carcinoma specimens and the matched normal esophageal mucosa samples by RT-PCR and immunohistochemistry method, respectively.
  
  
  RESULTS
  The positive expression rate of NS protein in normal esophageal mucosa, atypical hyperplasia and esophageal squamous cell carcinoma was 17.7% (11/62), 41.9% (13/31) and 69.4% (43/62), respectively. There was a significant difference among the above three groups (chi2 = 33.676, P < 0.01). The expression levels of NS mRNA in esophageal squamous cell carcinoma (0.971 +/- 0.121) was significantly higher than that in the atypical hyperplasia (0.913 +/- 0.085) and also in the normal esophageal mucosa (0.866 +/- 0.103; F = 14.829, P < 0.01). The expression level of both NS protein and mRNA was positively correlated with histological grade, infiltration depth, and lymph node metastasis (P < 0.05), but not with age, gender or pathological type (P > 0.05).
  
  
  CONCLUSIONS
  Our results indicate that nucleostemin mRNA and protein are over-expressed in human esophageal squamous cell carcinoma, and it may be related with its oncogenesis.
authors.affiliation: Department of Histology and Embryology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Gong-Yuan
authors.initials: GY
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Yin
authors.forename: Sheng-Lei
authors.initials: SL
authors.lastname: Li
authors.forename: Wen-Ying
authors.initials: WY
authors.lastname: Xing
authors.forename: Qiu-Min
authors.initials: QM
authors.lastname: Zhao
authors.forename: Xiao-Ping
authors.initials: XP
authors.lastname: Le
authors.forename: Dong-Ling
authors.initials: DL
authors.lastname: Gao
authors.forename: Kui-Sheng
authors.initials: KS
authors.lastname: Chen
authors.forename: Yun-Han
authors.initials: YH
authors.lastname: Zhang
authors.forename: Qin-Xian
authors.initials: QX
authors.lastname: Zhang
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D002352:Carrier Proteins / Q000096:biosynthesis* / Q000235:genetics; D004938:Esophageal Neoplasms / Q000378:metabolism* / Q000473:pathology; D004947:Esophagus / Q000473:pathology*; D005260:Female; D019204:GTP-Binding Proteins; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006965:Hyperplasia; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009092:Mucous Membrane / Q000378:metabolism; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000096:biosynthesis* / Q000235:genetics; D011230:Precancerous Conditions / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D002352:Carrier Proteins; C469949:GNL3 protein, human; D009687:Nuclear Proteins; D012333:RNA, Messenger; D019204:GTP-Binding Proteins