title: A new device to control mechanical environment in bone defect healing in rats.
abstract: Mechanical conditions have a significant influence on the biological processes of bone healing. Small animal models that allow controlling the mechanical environment of fracture and bone defect healing are needed. The aim of this study was to develop a new animal model that allows to reliably control the mechanical environment in fracture and bone defect healing in rats using different implant materials. An external fixator was designed and mounted in vitro to rat femurs using four Kirschner-wires (titanium (T) or steel (S)) of 1.2 mm diameter. The specimens were distracted to a gap of 1.5mm. Axial and torsional stiffness of the device was tested increasing the offset (distance between bone and fixator crossbar) from 5 to 15 mm. In vivo performance (well-being, infection, breaking of wires and bone healing) was evaluated in four groups of 24 Sprague-Dawley rats varying in offset (7.5 and 15 mm) and implant material (S/T) over 6 weeks. Torsional and axial stiffness were higher in steel compared to titanium setups. A decrease in all configurations was observed by increasing the offset. The offset 7.5 mm showed a significantly higher torsional (S: p<0.01, T: p<0.001) and axial in vitro stiffness (S: p<0.001, T: p<0.001) compared to 15 mm offset of the fixator. Although in vitro designed to be different in mechanical stiffness, no difference was found between the groups regarding complication rate. The overall-complication rate was 5.2%. In conclusion, we were able to establish a small animal model for bone defect healing which allows modeling the mechanical conditions at the defect site in a defined manner.
authors.affiliation: Center for Musculoskeletal Surgery Berlin, Charité-Universitätsmedizin Berlin, Clinic for Orthopedics, Chariteplatz 1, 10117 Berlin, Germany. patrick.strube@charite.de
authors.forename: Patrick
authors.initials: P
authors.lastname: Strube
authors.forename: Manav
authors.initials: M
authors.lastname: Mehta
authors.forename: Michael
authors.initials: M
authors.lastname: Putzier
authors.forename: Georg
authors.initials: G
authors.lastname: Matziolis
authors.forename: Carsten
authors.initials: C
authors.lastname: Perka
authors.forename: Georg N
authors.initials: GN
authors.lastname: Duda
mesh_terms: D000818:Animals; D055592:Biophysical Phenomena; D055119:Elastic Modulus; D004867:Equipment Design; D019544:Equipment Failure Analysis; D005260:Female; D005264:Femoral Fractures / Q000503:physiopathology* / Q000628:therapy*; D005592:Fracture Fixation / Q000295:instrumentation* / Q000379:methods; D017102:Fracture Healing / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013314:Stress, Mechanical