Detection and identification of proteins using nanoparticle-fluorescent polymer 'chemical nose' sensors.
A sensor array containing six non-covalent gold nanoparticle-fluorescent polymer conjugates has been created to detect, identify and quantify protein targets. The polymer fluorescence is quenched by gold nanoparticles; the presence of proteins disrupts the nanoparticle-polymer interaction, producing distinct fluorescence response patterns. These patterns are highly repeatable and are characteristic for individual proteins at nanomolar concentrations, and can be quantitatively differentiated by linear discriminant analysis (LDA). Based on a training matrix generated at protein concentrations of an identical ultraviolet absorbance at 280 nm (A280 = 0.005), LDA, combined with ultraviolet measurements, has been successfully used to identify 52 unknown protein samples (seven different proteins) with an accuracy of 94.2%. This work demonstrates the construction of novel nanomaterial-based protein detector arrays with potential applications in medical diagnostics.
You
Chang-Cheng
CC
Department of Chemistry, University of Massachusetts, 710 North Pleasant Street, Amherst, Massachusetts 01003, USA.
Miranda
Oscar R
OR
Gider
Basar
B
Ghosh
Partha S
PS
Kim
Ik-Bum
IB
Erdogan
Belma
B
Krovi
Sai Archana
SA
Bunz
Uwe H F
UH
Rotello
Vincent M
VM
D032701:Biomimetics / Q000295:instrumentation*; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D019544:Equipment Failure Analysis; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D036103:Nanotechnology / Q000295:instrumentation*; D009666:Nose / Q000502:physiology*; D011108:Polymers / Q000737:chemistry; D011506:Proteins / Q000032:analysis*; D013050:Spectrometry, Fluorescence / Q000379:methods*; D014159:Transducers
D011108:Polymers; D011506:Proteins