[The effects of ADAM28 on HDPC biological characteristics].
OBJECTIVE
To study the effects of ADAM28 gene on the proliferation, differentiation and apoptosis of human dental papilla cells(HDPC) and analyze the possible mechanism.


METHODS
Cell culture, gene transfection, MTT, flow cytometry (FCM) and enzyme kinetics method were used to study the effects of ADAM28 on HDPC biological characteristics. SNK test of SPSS12.0 software package was used for statistical analysis.


RESULTS
ADAM28 eukaryotic plasmid was transfected into HDPC successfully, HDPC proliferation activity, proliferation index(PI) and ALP secretion activity in eukaryotic plasmid group were significantly higher than those in pcDNA3.1(+) group, untransfected group, and apoptosis cell percentage in eukaryotic plasmid group was lower than that in the control groups.There were significant differences between them (P<0.01).


CONCLUSIONS
ADAM28 could significantly promote the proliferation and ALP secretion of HDPC, inhibit HDPC apoptosis. Supported by National Natural Science Foundation of China (Grant No.30572046).
Zhao
Zheng
Z
Department of Stomatology, The 401st Hospital of Jinan Military Area, PLA. Qingdao 266071, China. zhaozheng170@sohu.com
Xu
Jun-Ming
JM
Zhao
Wei-Li
WL
D051722:ADAM Proteins / Q000502:physiology*; D017209:Apoptosis; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D006801:Humans; D010957:Plasmids; D014070:Tooth / Q000166:cytology*; D014162:Transfection
D051722:ADAM Proteins; C410224:ADAM28 protein, human