The three mitochondrial encoded CcmF proteins form a complex that interacts with CCMH and c-type apocytochromes in Arabidopsis.
Three reading frames called ccmF(N1), ccmF(N2), and ccmF(c) are found in the mitochondrial genome of Arabidopsis. These sequences are similar to regions of the bacterial gene ccmF involved in cytochrome c maturation. ccmF genes are always absent from animal and fungi genomes but are found in mitochondrial genomes of land plant and several evolutionary distant eukaryotes. In Arabidopsis, ccmF(N2) despite the absence of a classical initiation codon is not a pseudo gene. The 3 ccmF genes of Arabidopsis are expressed at the protein level. Their products are integral proteins of the mitochondrial inner membrane with in total 11 to 13 predicted transmembrane helices. The conserved WWD domain of CcmF(N2) is localized in the inter membrane space. The 3 CcmF proteins are all detected in a high molecular mass complex of 500 kDa by Blue Native PAGE. Direct interaction between CcmF(N2) and both CcmF(N1) and CcmF(C) is shown with the yeast two-hybrid split ubiquitin system, but no interaction is observed between CcmF(N1) and CcmF(C). Similarly, interaction is detected between CcmF(N2) and apocytochrome c but also with apocytochrome c(1). Finally, CcmF(N1) and CcmF(N2) both interact with CCMH previously shown to interact as well with cytochrome c. This strengthens the hypothesis that CcmF and CCMH make a complex that performs the assembly of heme with c-type apocytochromes in plant mitochondria.
Rayapuram
Naganand
N
Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du général Zimmer, 67084 Strasbourg, France.
Hagenmuller
Jérémie
J
Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du général Zimmer, 67084 Strasbourg, France.
Grienenberger
Jean Michel
JM
Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du général Zimmer, 67084 Strasbourg, France.
Bonnard
Géraldine
G
Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du général Zimmer, 67084 Strasbourg, France.
Giegé
Philippe
P
Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du général Zimmer, 67084 Strasbourg, France. Electronic address: Philippe.Giege@ibmp-ulp.u-strasbg.fr.
D000818:Animals; D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000737:chemistry*; D003062:Codon; D045304:Cytochromes c / Q000737:chemistry*; D017343:Genes, Plant; D008565:Membrane Proteins / Q000737:chemistry* / Q000502:physiology; D008928:Mitochondria / Q000378:metabolism; D024101:Mitochondrial Proteins / Q000737:chemistry* / Q000502:physiology; D050862:Oxidoreductases Acting on Sulfur Group Donors / Q000737:chemistry*; D011485:Protein Binding; D017434:Protein Structure, Tertiary; D011817:Rabbits; D013347:Subcellular Fractions / Q000378:metabolism; D020798:Two-Hybrid System Techniques; D025801:Ubiquitin / Q000737:chemistry
D029681:Arabidopsis Proteins; D003062:Codon; D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D025801:Ubiquitin; D045304:Cytochromes c; C506270:CCMH protein, Arabidopsis; D050862:Oxidoreductases Acting on Sulfur Group Donors