title: Analysis of genetic and virulence diversity of Cylindrocarpon liriodendri and C. macrodidymum associated with black foot disease of grapevine.
abstract: Inter-simple sequence repeat (ISSR) analysis was used to investigate the genetic diversity of 87 Cylindrocarpon liriodendri and C. macrodidymum isolates, the causal agents of black foot disease of grapevine. The four ISSR primers (GT)7, (CCA)5, (CGA)5 and (TCG)5, were able to provide reproducible and polymorphic DNA fingerprint patterns and detected relevant genetic diversity in C. macrodidymum. The cluster analysis of ISSR data showed 21 different genotypes that were grouped in seven ISSR groups, from which two corresponded to C. liriodendri (G1 and G2) and five to C. macrodidymum (G3-G7). Nineteen isolates selected from the seven ISSR groups were inoculated in grapevine seedlings obtained from cv. 'Tempranillo'. The pathogenicity tests detected virulence diversity in C. macrodidymum. The isolates belonging to ISSR groups G6 and G7 were significantly more virulent than the other C. macrodidymum and C. liriodendri isolates.
authors.affiliation: Instituto Agroforestal Mediterráneo, Universidad Politécnica de Valencia, Camino de Vera s/n, 46022-Valencia, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Alaniz
authors.forename: Josep
authors.initials: J
authors.lastname: Armengol
authors.forename: Maela
authors.initials: M
authors.lastname: León
authors.forename: José
authors.initials: J
authors.lastname: García-Jiménez
authors.forename: Paloma
authors.initials: P
authors.lastname: Abad-Campos
mesh_terms: D016172:DNA Fingerprinting; D017931:DNA Primers; D004271:DNA, Fungal / Q000032:analysis; D014644:Genetic Variation; D006999:Hypocreales / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D010935:Plant Diseases / Q000382:microbiology*; D016133:Polymerase Chain Reaction / Q000379:methods; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics; D014774:Virulence; D027843:Vitis / Q000382:microbiology*
chemical_list: D017931:DNA Primers; D004271:DNA, Fungal