Molecular and biochemical characterization of SHV-56, a novel inhibitor-resistant beta-lactamase from Klebsiella pneumoniae.
A clinical strain of Klebsiella pneumoniae was found to possess the chromosomal gene bla(SHV-56), encoding a new inhibitor-resistant beta-lactamase with a pI of 7.6. SHV-56 is derived from SHV-11 by the single substitution K234R. This mutation therefore evidences a new critical site for inhibitor resistance among SHV enzymes.
Dubois
Véronique
V
Laboratoire de Microbiologie, UMR-CNRS 5234, Université de Bordeaux 2, 146 Rue Léo Saignat, 33076 Bordeaux Cedex, France. veronique.dubois@bacterio.u-bordeaux2.fr
Poirel
Laurent
L
Demarthe
François
F
Arpin
Corinne
C
Coulange
Laure
L
Minarini
Luciene A R
LA
Bezian
Marie-Christine
MC
Nordmann
Patrice
P
Quentin
Claudine
C
D000368:Aged; D019943:Amino Acid Substitution; D001483:Base Sequence; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial; D006801:Humans; D007526:Isoelectric Point; D007700:Kinetics; D007710:Klebsiella Infections / Q000188:drug therapy / Q000382:microbiology; D007711:Klebsiella pneumoniae / Q000201:enzymology* / Q000235:genetics* / Q000302:isolation & purification; D008969:Molecular Sequence Data; D016254:Mutagenesis, Insertional; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D018440:beta-Lactam Resistance / Q000235:genetics; D001618:beta-Lactamases / Q000737:chemistry* / Q000235:genetics* / Q000378:metabolism
D017931:DNA Primers; D004269:DNA, Bacterial; D011994:Recombinant Proteins; C107197:beta-lactamase SHV-11; D001618:beta-Lactamases