Sensitive determination of biogenic amines by capillary electrophoresis with a new fluorogenic reagent 3-(4-fluorobenzoyl)-2-quinolinecarboxaldehyde.
An effective approach was proposed to the derivatization of seven biogenic amines using 3-(4-fluorobenzoyl)-2-quinolinecarboxaldehyde (FBQCA) as a fluorogenic reagent. The sensitive determinations of these derivatives were achieved by micellar electrokinetic capillary chromatography (MEKC) with laser-induced fluorescence (LIF) detection. The derivatization and electrophoretic conditions have been optimized. A running buffer was composed of mixtures of 25 mM pH 9.5 boric acid, 25 mM SDS, and 27% ACN. At 25 degrees C and 22.5 kV, the baseline separation of the derivatives was accomplished in 13 min. The detection limit (S/N=3) was found as low as 0.4 nM. The proposed method was validated by the linearity of two orders magnitude and correlation coefficient in the range 0.9969-0.9998. Also, the procedure was successfully applied to the determination of biogenic amines in soy sauce, fish and wine samples.
Zhang
Niu
N
Department of Chemistry, Wuhan University, Wuhan 430075, PR China.
Wang
Hong
H
Zhang
Zi-Xing
ZX
Deng
Ying-Hua
YH
Zhang
Hua-Shan
HS
D001565:Benzoates / Q000494:pharmacology; D001679:Biogenic Amines / Q000032:analysis* / Q000737:chemistry; D002845:Chromatography / Q000379:methods; D004563:Electrochemistry / Q000379:methods; D019075:Electrophoresis, Capillary / Q000295:instrumentation* / Q000379:methods*; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000494:pharmacology; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D007834:Lasers; D008823:Micelles; D011804:Quinolines / Q000494:pharmacology; D015203:Reproducibility of Results; D013696:Temperature
D001565:Benzoates; D001679:Biogenic Amines; D005456:Fluorescent Dyes; D008823:Micelles; D011804:Quinolines; C069617:3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde