Simultaneously subtyping of all influenza A viruses using DNA microarrays.
Rapid diagnosis of novel emerging subtypes of influenza viruses is vital for effective global influenza surveillance. To this end, a novel microarray based surveillance was developed for subtyping all influenza A viruses on one chip. Using reference strains of different influenza subtypes and samples from different areas, the results show that all the subtypes of the influenza A virus could be identified simultaneously on this microchip with high sensitivity. There was no cross-hybridization reaction with other viruses, indicating that the microarray is specific for influenza A viruses. Such a diagnostic microarray will undoubtedly be useful for identifying novel influenza A virus subtypes.
Han
Xueqing
X
Chinese Academy of Inspection and Quarantine, Beijing 100029, China. hanxueq@yahoo.com.cn
Lin
Xiangmei
X
Liu
Bohua
B
Hou
Yihong
Y
Huang
Jinhai
J
Wu
Shaoqiang
S
Liu
Jian
J
Mei
Lin
L
Jia
Guangle
G
Zhu
Qingyu
Q
D000818:Animals; D001717:Birds; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics*; D005585:Influenza in Birds / Q000821:virology; D007251:Influenza, Human / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D012680:Sensitivity and Specificity
D004279:DNA, Viral; D012367:RNA, Viral