Real-time electrochemical detection of extracellular nitric oxide in tobacco cells exposed to cryptogein, an elicitor of defence responses.
It was previously reported that cryptogein, an elicitor of defence responses, induces an intracellular production of nitric oxide (NO) in tobacco. Here, the possibility was explored that cryptogein might also trigger an increase of NO extracellular content through two distinct approaches, an indirect method using the NO probe 4,5-diaminofluorescein (DAF-2) and an electrochemical method involving a chemically modified microelectrode probing free NO in biological media. While the chemical nature of DAF-2-reactive compound(s) is still uncertain, the electrochemical modified microelectrodes provide real-time evidence that cryptogein induces an increase of extracellular NO. Direct measurement of free extracellular NO might offer important new insights into its role in plants challenged by biotic stresses.
Besson-Bard
Angélique
A
UMR INRA 1088/CNRS 5184/Université de Bourgogne, Plante-Microbe-Environnement, BP 86510, 17 rue Sully, 21000 Dijon, France.
Griveau
Sophie
S
Bedioui
Fethi
F
Wendehenne
David
D
D020418:Algal Proteins / Q000494:pharmacology*; D002478:Cells, Cultured; D004563:Electrochemistry / Q000379:methods*; D005656:Fungal Proteins; D009569:Nitric Oxide / Q000378:metabolism*; D014026:Nicotiana / Q000187:drug effects* / Q000378:metabolism*
D020418:Algal Proteins; D005656:Fungal Proteins; C061032:cryptogein protein, Phytophthora cryptogea; D009569:Nitric Oxide