Arterial baroreflex function is an important determinant of acute cerebral ischemia in rats with middle cerebral artery occlusion.
OBJECTIVE
To clarify whether arterial baroreflex function is an important determinant of acute cerebral ischemia in rats.


METHODS
Three animal models were used in this study. In the first, saponin conjugated with substance P (SP-SAP) was injected into the nucleus tractus solitarii (NTS) of Sprague-Dawley (SD) rats to block the central baroreflex arc. In the second model, sinoaortic denervation (SAD) was performed to destroy the peripheral baroreflex arc in SD rats. In the third model, SD rats were divided into two groups according to their naturally occurring BRS values. After determining hemodynamic indexes and baroreflex sensitivity (BRS), we subjected the animals to middle cerebral artery (MCA) occlusion. Levels of interleukin (IL)-1beta and IL-6 were detected both in SAD/sham operation groups and low/high BRS groups.


RESULTS
In all three animal models, baroreflex dysfunction significantly increased the infarct volume and weight. The levels of inflammatory factors were markedly elevated in SAD and low BRS groups.


CONCLUSIONS
These results demonstrate that the function of arterial baroreflex is an important determinant of acute cerebral ischemia in rats with MCA occlusion. Inflammation might be an important mechanism for the arterial baroreflex dysfunction-induced increase in brain damage in rats with MCA occlusion.
Liu
Ai-Jun
AJ
Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Ling
Gang
G
Wu
Jian
J
Shen
Fu-Ming
FM
Wang
Di-Song
DS
Lin
Li-Li
LL
Liu
Jian-Guo
JG
Su
Ding-Feng
DF
D000208:Acute Disease; D000818:Animals; D017704:Baroreflex / Q000502:physiology*; D001794:Blood Pressure / Q000502:physiology; D001923:Brain Chemistry / Q000502:physiology; D002545:Brain Ischemia / Q000503:physiopathology*; D002536:Cerebral Arteries / Q000503:physiopathology*; D003714:Denervation; D006339:Heart Rate / Q000502:physiology; D020244:Infarction, Middle Cerebral Artery / Q000473:pathology; D007267:Injections; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D020768:Middle Cerebral Artery / Q000473:pathology / Q000502:physiology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012503:Saponins / Q000008:administration & dosage / Q000494:pharmacology; D012849:Sinoatrial Node / Q000502:physiology; D017552:Solitary Nucleus / Q000502:physiology; D013373:Substance P / Q000008:administration & dosage / Q000494:pharmacology
D053583:Interleukin-1beta; D015850:Interleukin-6; D012503:Saponins; D013373:Substance P