[Osteogenic and adipogenic capacity of fibroblast-like progenitor cells derived frow human fetal liver].
The study of differentiation potential of multiponent stromal progenitor cells (PCs) in embryogenesis is a crucial issue for understanding their biology and role in tissue regeneration of an adult organism. In this study in monolayer culture there were investigated osteogenic and adipogenic capacities of fibroblast-like PCs derived from human fetal liver of 8-11 gestation weeks before and after exposure to cryoprotectant dimethyl sulphoxide (DMSO). It was shown that the primary suspension of human fetal liver cells included immature stromal fibroblast-like PCs which were able to be induced into osteogenic and adipogenic differentiation. A short-time exposure of freshly isolated human fetal liver cells to cryoprotectant DMSO led to altering properties of the fibroblast-like PCs. Under subculture conditions, it was found an increase in the number of fibroblast-like PCs which were able to be induced to osteogenic differentiation in vitro. The established fact of DMSO influence on the differentiation capacity of fetal fibroblast-like PCs is necessary to take into consideration while developing cryopreservation methods for stem cells.
Skorobogatova
N G
NG
Volkova
N A
NA
Petrenko
A Iu
AIu
D050156:Adipogenesis; D002454:Cell Differentiation; D002478:Cells, Cultured; D003451:Cryoprotective Agents / Q000494:pharmacology; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D005333:Fetus / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D008099:Liver / Q000166:cytology; D039902:Multipotent Stem Cells; D010012:Osteogenesis; D013234:Stem Cells / Q000187:drug effects / Q000502:physiology*
D003451:Cryoprotective Agents; D004121:Dimethyl Sulfoxide