title: A convenient one-step synthesis of L-aminotryptophans and improved synthesis of 5-fluorotryptophan.
abstract: A one-pot biotransformation for the generation of a series of L-aminotryptophans using a readily prepared protein extract containing tryptophan synthase is reported. The extract exhibits remarkable stability upon freeze-drying, and may be stored and used for long periods after its preparation without significant loss of activity.
authors.affiliation: School of Chemical Sciences and Pharmacy, University of East Anglia, Norwich NR4 7TJ, UK.
authors.forename: Michael
authors.initials: M
authors.lastname: Winn
authors.forename: Abhijeet Deb
authors.initials: AD
authors.lastname: Roy
authors.forename: Sabine
authors.initials: S
authors.lastname: Grüschow
authors.forename: Raj S
authors.initials: RS
authors.lastname: Parameswaran
authors.forename: Rebecca J M
authors.initials: RJ
authors.lastname: Goss
mesh_terms: D001671:Biochemistry / Q000379:methods*; D001711:Biotransformation; D002625:Chemistry, Organic / Q000379:methods; D004339:Drug Compounding; D015195:Drug Design; D004355:Drug Stability; D005612:Freeze Drying; D008956:Models, Chemical; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000138:chemical synthesis* / Q000737:chemistry; D014367:Tryptophan Synthase / Q000737:chemistry
chemical_list: C026927:5-fluorotryptophan; D014364:Tryptophan; D014367:Tryptophan Synthase