title: A case of transfusion-transmitted hepatitis E caused by blood from a donor infected with hepatitis E virus via zoonotic food-borne route.
abstract: BACKGROUND
  Five cases of transfusion transmission of hepatitis E virus (HEV) have been reported so far. The infection routes of the causative donors remain unclear, however. Also, the progress of virus markers in the entire course of HEV infection has not been well documented.
  
  
  METHODS
  Nucleic acid testing was performed by real-time reverse transcription-polymerase chain reaction targeting the open reading frame 2 region of HEV. Full-length nucleotide sequences of HEV RNA were detected by direct sequencing.
  
  
  RESULTS
  Lookback study of a HEV-positive donor revealed that the platelets (PLTs) donated from him 2 weeks previously contained HEV RNA and were transfused to a patient. Thirteen relatives including the donor were ascertained to enjoy grilled pork meats together in a barbecue restaurant 23 days before the donation. Thereafter, his father died of fulminant hepatitis E and the other 6 members showed serum markers of HEV infection. In the recipient, HEV was detected in serum on Day 22 and reached the peak of 7.2 log copies per mL on Day 44 followed by the steep increase of alanine aminotransferase. Immunoglobulin G anti-HEV emerged on Day 67; subsequently, hepatitis was resolved. HEV RNA sequences from the donor and recipient were an identical, Japan-indigenous strain of genotype 4. HEV RNA was detectable up to Day 97 in serum, Day 85 in feces, and Day 71 in saliva.
  
  
  CONCLUSIONS
  A transfusion-transmitted hepatitis E case by blood from a donor infected via the zoonotic food-borne route and the progress of HEV markers in the entire course are demonstrated. Further studies are needed to clarify the epidemiology and the transfusion-related risks for HEV even in industrialized countries.
authors.affiliation: Hokkaido Red Cross Blood Center, Sapporo, Japan. kmatsu@hokkaido.bc.jrc.or.jp
authors.forename: Keiji
authors.initials: K
authors.lastname: Matsubayashi
authors.forename: Jong-Hon
authors.initials: JH
authors.lastname: Kang
authors.forename: Hidekatsu
authors.initials: H
authors.lastname: Sakata
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Takahashi
authors.forename: Motohiro
authors.initials: M
authors.lastname: Shindo
authors.forename: Masaru
authors.initials: M
authors.lastname: Kato
authors.forename: Shinichiro
authors.initials: S
authors.lastname: Sato
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Kato
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nishimori
authors.forename: Kunihiko
authors.initials: K
authors.lastname: Tsuji
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Maguchi
authors.forename: Jun-Ichi
authors.initials: J
authors.lastname: Yoshida
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maekubo
authors.forename: Shunji
authors.initials: S
authors.lastname: Mishiro
authors.forename: Hisami
authors.initials: H
authors.lastname: Ikeda
mesh_terms: D000328:Adult; D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D001782:Blood Donors; D016751:Hepatitis E / Q000097:blood / Q000635:transmission / Q000821:virology*; D016752:Hepatitis E virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood; D008297:Male; D008460:Meat / Q000821:virology; D008875:Middle Aged; D008969:Molecular Sequence Data; D010802:Phylogeny; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D013552:Swine; D013553:Swine Diseases / Q000821:virology*; D013997:Time Factors; D065227:Transfusion Reaction
chemical_list: D000914:Antibodies, Viral; D007074:Immunoglobulin G; D012367:RNA, Viral