title: Postthaw survival of rhesus macaque sperm: variation in the response of individual males to different freezing protocols.
abstract: This study was designed to assess the effects of various cryopreservation protocols on the postthaw survival of rhesus macaque sperm. Multiple ejaculates of five mature males were collected and frozen by each of four different methods. Cryopreservation significantly reduced motility in all samples, regardless of the method, and the response of different ejaculates of the same male to each method proved to be consistent. The freezing method was a significant factor in determining the immediate postthaw motility, although this effect disappeared after 4 hr. Moreover, there was a significant interaction between individual males and freezing method suggesting that developing a single freezing protocol that is universally suitable may be difficult.
authors.affiliation: Tulane National Primate Research Center, Covington, Louisiana 70433, USA.
authors.forename: Christine
authors.initials: C
authors.lastname: Gagliardi
authors.forename: Leann
authors.initials: L
authors.lastname: Myers
authors.forename: H Michael
authors.initials: HM
authors.lastname: Kubisch
mesh_terms: D000818:Animals; D015925:Cryopreservation; D005838:Genotype; D008253:Macaca mulatta / Q000235:genetics; D008297:Male; D012662:Semen Preservation; D013081:Sperm Motility; D013094:Spermatozoa / Q000502:physiology*