Topology of a ternary complex (proparacaine-beta-cyclodextrin-liposome) by STD NMR.
The topologies of proparacaine (PPC) in beta-cyclodextrin (beta-CD), PPC in egg phosphatidylcholine (EPC) liposomes and PPC in beta-CD in EPC were investigated using NMR experiments (1D ROESY and saturation transfer difference (STD)). This is the first description of the STD technique applied to PPC-EPC-beta-CD system, revealing that not only PPC was imbedded in EPC bilayer, but beta-CD was also interacting with liposome vesicles. These results are novel and were rationalized as the spontaneous formation of a ternary complex with some beta-CD molecules bound to external liposome vesicles surfaces.
Cabeça
Luís Fernando
LF
Institute of Chemistry, UNICAMP, CP 6154, 13083-970 Campinas, SP, Brazil.
Fernandes
Sergio Antonio
SA
de Paula
Eneida
E
Marsaioli
Anita Jocelyne
AJ
D001665:Binding Sites; D004058:Diffusion; D008081:Liposomes / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy / Q000379:methods* / Q000592:standards*; D015394:Molecular Structure; D011430:Propoxycaine / Q000737:chemistry*; D012015:Reference Standards; D047392:beta-Cyclodextrins / Q000737:chemistry*
D008081:Liposomes; D047392:beta-Cyclodextrins; C005717:proxymetacaine; D011430:Propoxycaine; C031215:betadex