title: Supplemental sodium butyrate stimulates different gastric cells in weaned pigs.
abstract: Sodium butyrate (SB) is used as an acidifier in animal feed. We hypothesized that supplemental SB impacts gastric morphology and function, depending on the period of SB provision. The effect of SB on the oxyntic and pyloric mucosa was studied in 4 groups of 8 pigs, each supplemented with SB either during the suckling period (d 4-28 of age), after weaning (d 29 to 39-40 of age) or both, or never. We assessed the number of parietal cells immunostained for H+/K+-ATPase, gastric endocrine cells immunostained for chromogranin A and somatostatin (SST) in the oxyntic mucosa, and gastrin-secreting cells in the pyloric mucosa. Gastric muscularis and mucosa thickness were measured. Expressions of the H+/K+-ATPase and SST type 2 receptor (SSTR2) genes in the oxyntic mucosa and of the gastrin gene in the pyloric mucosa were evaluated by real-time RT-PCR. SB increased the number of parietal cells per gland regardless of the period of administration (P < 0.05). SB addition after, but not before, weaning increased the number of enteroendocrine and SST-positive cells (P < 0.01) and tended to increase gastrin mRNA (P = 0.09). There was an interaction between the 2 periods of SB treatment for the expression of H/K-ATPase and SSTR2 genes (P < 0.05). Butyrate intake after weaning increased gastric mucosa thickness (P < 0.05) but not muscularis. SB used orally at a low dose affected gastric morphology and function, presumably in relationship with its action on mucosal maturation and differentiation.
authors.affiliation: Department of Agri-food Protection and Improvement, University of Bologna, 42100 Reggio Emilia, Italy.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Mazzoni
authors.forename: Maud
authors.initials: M
authors.lastname: Le Gall
authors.forename: Sara
authors.initials: S
authors.lastname: De Filippi
authors.forename: Laura
authors.initials: L
authors.lastname: Minieri
authors.forename: Paolo
authors.initials: P
authors.lastname: Trevisi
authors.forename: Jaroslaw
authors.initials: J
authors.lastname: Wolinski
authors.forename: Giovanna
authors.initials: G
authors.lastname: Lalatta-Costerbosa
authors.forename: Jean-Paul
authors.initials: JP
authors.lastname: Lallès
authors.forename: Paul
authors.initials: P
authors.lastname: Guilloteau
authors.forename: Paolo
authors.initials: P
authors.lastname: Bosi
mesh_terms: D000821:Animal Feed; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000833:Animals, Suckling; D020148:Butyric Acid / Q000494:pharmacology*; D004032:Diet / Q000662:veterinary; D019587:Dietary Supplements; D019858:Enteroendocrine Cells / Q000187:drug effects / Q000378:metabolism; D005753:Gastric Mucosa / Q000166:cytology* / Q000187:drug effects* / Q000378:metabolism; D005755:Gastrins / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D017506:H(+)-K(+)-Exchanging ATPase / Q000235:genetics / Q000378:metabolism; D017481:Receptors, Somatostatin / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000502:physiology*; D014886:Weaning
chemical_list: D005755:Gastrins; D017481:Receptors, Somatostatin; D020148:Butyric Acid; C093733:somatostatin receptor 2; D017506:H(+)-K(+)-Exchanging ATPase