title: Superparamagnetic iron oxide does not affect the viability and function of adipose-derived stem cells, and superparamagnetic iron oxide-enhanced magnetic resonance imaging identifies viable cells.
abstract: The objectives of this study were (1) to determine whether superparamagnetic iron oxide (SPIO) affects viability, transdifferentiation potential and cell-factor secretion of adipose-derived stem cells (ASCs); and (2) to determine whether SPIO-enhanced magnetic resonance (MR) imaging highlights living stem cells. Rat ASCs were incubated in SPIO-containing cell culture medium for 2 days. The SPIO-treated ASCs were then subjected to adipogenic, osteogenic and myogenic transdifferentiation. Expression of vascular endothelial growth factor, hepatocyte growth factor and insulin-like growth factor 1 by the SPIO-treated ASCs was measured using reverse transcription polymerase chain reaction. Cell viability was assessed using trypan blue stain. For in vivo experiments, SPIO-labeled ASCs were injected into 10 rat hearts. The hearts were monitored using MRI. We found that survival rate of the ASCs cultured in the SPIO-containing medium was very high (97-99%). The SPIO-treated ASCs continued to express specific markers for the three types of transdifferentiation. Expression of the cell factors by the ASCs was not affected by SPIO. Signal voids on MR images were associated with the living SPIO-labeled ASCs in the rat hearts. We conclude that SPIO does not affect viability, transdifferentiation potential or cell-factor secretion of ASCs. MRI mainly highlights living SPIO-labeled stem cells.
authors.affiliation: Institute for Biodiagnostics, National Research Council Canada, Winnipeg, Canada R3B 1Y6.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Jixian
authors.initials: J
authors.lastname: Deng
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Bo
authors.initials: B
authors.lastname: Xiang
authors.forename: Tonghua
authors.initials: T
authors.lastname: Yang
authors.forename: Marco
authors.initials: M
authors.lastname: Gruwel
authors.forename: Tarek
authors.initials: T
authors.lastname: Kashour
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Tomanek
authors.forename: Randy
authors.initials: R
authors.lastname: Summer
authors.forename: Darren
authors.initials: D
authors.lastname: Freed
authors.forename: Davinder S
authors.initials: DS
authors.lastname: Jassal
authors.forename: Guangping
authors.initials: G
authors.lastname: Dai
authors.forename: Miriam
authors.initials: M
authors.lastname: Glogowski
authors.forename: Roxanne
authors.initials: R
authors.lastname: Deslauriers
authors.forename: Rakesh C
authors.initials: RC
authors.lastname: Arora
authors.forename: Ganghong
authors.initials: G
authors.lastname: Tian
mesh_terms: D017667:Adipocytes / Q000378:metabolism / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured; D003287:Contrast Media / Q000494:pharmacology*; D003911:Dextrans; D052203:Ferrosoferric Oxide; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007501:Iron / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D008297:Male; D010087:Oxides / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
chemical_list: D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide