Enhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster.
To enhance the heterologous production of eicosapentaenoic acid (EPA) in Escherichia coli, the EPA biosynthesis gene cluster from Shewanella oneidensis MR-1 was cloned under the lacZ promoter on a high-copy number plasmid, pBluescript SK+. The production of EPA was remarkably enhanced yielding levels of up to 7.5% of the total fatty acid content in the recombinant E. coli strain by induction with IPTG, whereas the stimulation of EPA production was abolished by adding glucose into the culture medium, probably due to glucose repression acting on the promoter activity.
Lee
Su-Jin
SJ
Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, South Korea.
Kim
Chul Ho
CH
Seo
Pil-Soo
PS
Kwon
Ohsuk
O
Hur
Byung-Ki
BK
Seo
Jeong-Woo
JW
D003001:Cloning, Molecular; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D015870:Gene Expression; D005798:Genes, Bacterial; D007763:Lac Operon; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D020592:Shewanella / Q000235:genetics* / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics
D015118:Eicosapentaenoic Acid; D017855:Transferases (Other Substituted Phosphate Groups); C021364:holo-(acyl-carrier-protein) synthase