title: Mixed micellar liquid chromatography methods: modelling quantitative retention-activity relationships of angiotensin converting enzyme inhibitors.
abstract: The capability of biopartitioning micellar chromatography (BMC), using pure Brij35 solution and mixed micellar system of Brij35-SDS (85:15) as mobile phase, to describe and estimate bioactivities of angiotensin converting enzyme inhibitors at different pH has been studied. Quantitative retention-activity relationships (QRAR) in BMC were investigated for these compounds. The obtained BMC(Brij35-SDS)-QRAR models were compared with the traditional BMC(Brij35)-QRAR, and better statistically models were obtained using Brij35-SDS retention data. The superiority of BMC(Brij35-SDS)-QRAR is due to the fact that the mixed micellar mobile phase can simulate the resting membrane potential and the conformation of the long hydrophilic polyoxyethylene chains remains unchanged.
authors.affiliation: West China School of Pharmacy, Sichuan University, Chengdu, 610041, People's Republic of China.
authors.forename: Li-Ping
authors.initials: LP
authors.lastname: Wu
authors.forename: Yan
authors.initials: Y
authors.lastname: Cui
authors.forename: Mei-Jin
authors.initials: MJ
authors.lastname: Xiong
authors.forename: Shu-Rong
authors.initials: SR
authors.lastname: Wang
authors.forename: Cong
authors.initials: C
authors.lastname: Chen
authors.forename: Li-Ming
authors.initials: LM
authors.lastname: Ye
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D002853:Chromatography, Liquid / Q000379:methods*; D008823:Micelles; D008954:Models, Biological; D011092:Polyethylene Glycols / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D008823:Micelles; D011092:Polyethylene Glycols; C515901:Brij 35