Evaluation of the coupling between ultra performance liquid chromatography and evaporative light scattering detector for selected phytochemical applications.
Fast analysis in LC can be performed with sub-2 microm particles at very high pressures (up to 1000 bar) known as ultra performance LC (UPLC). With this configuration, it is possible to obtain fast and/or highly efficient separations compared to conventional LC. For the analysis of compounds without chromophores, the evaporative light scattering detector (ELSD) is an attractive alternative because of its quasi-universality, versatility, low-cost and good sensitivity. The UPLC-ELSD was investigated in terms of sensitivity and apparent efficiency, with a conventional ELSD instrument, for two types of commercially available nebulisers, using different mobile phase flow rates and column ids. Results were finally compared with the UPLC-UV configuration. Three applications with phytochemical compounds were selected to highlight the potential of this approach (i.e. the isocratic separations of artemisinin and its derivatives, of calystegines and the gradient separation of several tropane alkaloids). Depending on the used column length, baseline separations were obtained in 3-10 min, with an average apparent efficiency ranging from 7000 to 30,000 plates.
Russo
Rossana
R
Laboratory of Phytoanalysis, Dipartimento di Scienza e Tecnologia del Farmaco, Turin University, Turin, Italy.
Guillarme
Davy
D
Rudaz
Serge
S
Bicchi
Carlo
C
Veuthey
Jean-Luc
JL
D037621:Artemisinins / Q000737:chemistry / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods / Q000706:statistics & numerical data; D008027:Light; D009662:Nortropanes / Q000737:chemistry / Q000302:isolation & purification; D012542:Scattering, Radiation; D012680:Sensitivity and Specificity; D012991:Solanaceous Alkaloids / Q000737:chemistry / Q000302:isolation & purification; D014326:Tropanes / Q000737:chemistry / Q000302:isolation & purification
D037621:Artemisinins; D009662:Nortropanes; D012991:Solanaceous Alkaloids; D014326:Tropanes; C055033:calystegin; C031327:artemisinin