Basal budding and replication of the murine leukemia virus are independent of the gag L domains.
Moloney murine leukemia virus (MMuLV) Gag protein contains three identified late (L) domains, PPPY, YPAL, and PSAP, which are thought to interact with the endosomal sorting machinery to assist budding. We created single and combined L-domain mutants in all permutations and tested the resulting clones for budding and replication. Budding and replication of all viruses with mutated PPPY were greatly reduced; however, the basal replication level was retained, demonstrated by the slow spread of the viruses in culture. Mutations in PSAP or YPAL did not affect budding or spreading, demonstrating that these two motifs are dispensable for efficient MMuLV replication. Furthermore, the basal budding level was maintained following inhibition of endosomal sorting machinery, emphasizing that the basal budding of MMuLV is independent of this machinery.
Sabo
Yosef
Y
Department of Cell Research and Immunology, Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978, Israel.
Laham-Karam
Nihay
N
Bacharach
Eran
E
D020816:Amino Acid Motifs; D000818:Animals; D002460:Cell Line; D004261:DNA Replication; D015683:Gene Products, gag / Q000378:metabolism*; D005814:Genes, Viral; D015750:Genes, gag; D006801:Humans; D007700:Kinetics; D051379:Mice; D008954:Models, Biological; D008979:Moloney murine leukemia virus / Q000235:genetics*; D009154:Mutation; D017434:Protein Structure, Tertiary; D019065:Virus Assembly / Q000235:genetics
D015683:Gene Products, gag