title: [Inhibition of growth and proliferation of Hep-2 cells by targeting c-myc gene using small interference RNA technology].
abstract: OBJECTIVE
  To investigate the effect of small interfering RNA (siRNA) targeting c-myc gene in Hep-2 cells.
  
  
  METHODS
  siRNA targeting c-myc mRNA was designed and synthesized. In vitro cultured Hep-2 cells were transfected with lipofectamine 2000 and the inhibitory effect was detected by MTT, morphology, real time PCR assay.
  
  
  RESULTS
  1) The MTT result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication; 2) The real time PCR result showed c-myc at mRNA level inhibition ratio at 94% in group S3; 3) The morphology result showed the c-myc siRNA to be able effectively to suppress the Hep-2 cell multiplication, the cell heteromorphism was diminished.
  
  
  CONCLUSIONS
  siRNA targeting c-myc can remarkably suppress the Hep-2 cell growth and multiplication.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the First Hospital of Shunde, Foshan, 528300, China. damaodoctor@126.com
authors.forename: Zhi
authors.initials: Z
authors.lastname: Tang
authors.forename: Jiqun
authors.initials: J
authors.lastname: Wang
authors.forename: Xinyi
authors.initials: X
authors.lastname: Wu
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.forename: Xueli
authors.initials: X
authors.lastname: Lin
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Shan
mesh_terms: D017209:Apoptosis; D002455:Cell Division; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D018390:Gene Targeting; D016259:Genes, myc / Q000235:genetics*; D006801:Humans; D034741:RNA, Small Interfering / Q000235:genetics*; D014162:Transfection
chemical_list: D034741:RNA, Small Interfering