Markers of glycemic control in the mouse: comparisons of 6-h- and overnight-fasted blood glucoses to Hb A1c.
The present studies examined the relationship between fasting blood glucose and Hb A(1c) in C57BL/6J, DBA/2J, and KK/HlJ mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of 6-h vs. overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with Hb A(1c), as determined with a hand-held automated device using an immunodetection method. R(2) values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J, respectively. Six-hour fasting blood glucose correlated more closely with the level of daily averaged blood glucose and with Hb A(1c) than did blood glucose following an overnight fast. To validate the immunoassay-determined Hb A(1c), we also measured total glycosylated hemoglobin using boronate HPLC. Hb A(1c) values correlated well with total glycosylated hemoglobin in all three strains but were relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice. These results show that 6-h fasting glucose provides a superior index of glycemic control and correlates more closely with Hb A(1c) than overnight-fasted blood glucose in these strains of mice.
Han
Byoung Geun
BG
Department of Medicine, Vanderbilt University, Nashville, Tennessee, USA.
Hao
Chuan-Ming
CM
Tchekneva
Elena E
EE
Wang
Ying-Ying
YY
Lee
Chieh Allen
CA
Ebrahim
Benyamin
B
Harris
Raymond C
RC
Kern
Timothy S
TS
Wasserman
David H
DH
Breyer
Matthew D
MD
Qi
Zhonghua
Z
D000818:Animals; D015415:Biomarkers; D001786:Blood Glucose / Q000502:physiology*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism; D003928:Diabetic Nephropathies / Q000378:metabolism; D005215:Fasting / Q000502:physiology; D005947:Glucose / Q000378:metabolism; D006442:Glycated Hemoglobin / Q000032:analysis* / Q000378:metabolism*; D051379:Mice; D008810:Mice, Inbred C57BL; D008811:Mice, Inbred DBA; D011237:Predictive Value of Tests
D015415:Biomarkers; D001786:Blood Glucose; D005947:Glucose; D006442:Glycated Hemoglobin