Identification and quantitation of auxins in plants by liquid chromatography/electrospray ionization ion trap mass spectrometry.
Auxin is an important phylohormone, which regulates specific physiological responses such as division, elongation and differentiation of cells. A new method using liquid chromatography/electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) has been developed for identification and quantitation of four auxins. Under the optimum conditions, four auxins (indole-3-acetic acid, indole-3-propionic acid, indole-3-butyric acid and 1-naphthylacetic acid) were completely separated and quantitated within 7 min with a minimum detection limit of 8.0 ng mL(-1) with relative standard deviations lower than 5.0%. This method also has been applied to analysis of auxins in Chinese cabbage where, even with a complicated serious background perturbation due to the natural biological matrix, the mean recoveries ranged from 77.5% to 99.8%. Finally, we discuss the MS-relevant properties of the identified auxins in detail.
Lu
Qiaomei
Q
Ministry of Education Key Laboratory of Analysis and Detection for Food Safety (Fuzhou University), Fuzhou, Fujian 350002, China.
Zhang
Lan
L
Chen
Tianwen
T
Lu
Minghua
M
Ping
Tong
T
Chen
Guonan
G
D001937:Brassica / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D007210:Indoleacetic Acids / Q000737:chemistry*; D007211:Indoles / Q000737:chemistry; D009280:Naphthaleneacetic Acids / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D010937:Plant Growth Regulators / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
D007210:Indoleacetic Acids; D007211:Indoles; D009280:Naphthaleneacetic Acids; D010936:Plant Extracts; D010937:Plant Growth Regulators; C014612:indolebutyric acid; C034182:1-naphthaleneacetic acid; C030737:indoleacetic acid; C015292:indolepropionic acid