title: Herpes simplex virus 2 serostatus and viral loads of HIV-1 in blood and semen as risk factors for HIV transmission among men who have sex with men.
abstract: OBJECTIVE
  Human immunodeficiency virus type 1 blood plasma viral load is correlated with the sexual transmission of HIV, although transmission from men involves virus from semen instead of blood. We quantified HIV-1 RNA in the blood and semen of men who did or did not transmit HIV to their sex partners. We compared the relationships of HIV-1 transmission risk with blood plasma viral load, seminal plasma viral load, herpes simplex virus 2 serostatus and other factors.
  
  
  METHODS
  A case-control study.
  
  
  METHODS
  Participants were men evaluated for primary HIV infection and their recent male sex partners. They were interviewed, and clinical specimens were collected. Epidemiologic and phylogenetic linkages were determined by history and molecular techniques. Couples were grouped on the basis of transmission after exposure. Fisher's exact test and Wilcoxon tests were used for comparisons between groups. Multivariable logistic regressions were fit to identify independent predictors of transmission.
  
  
  RESULTS
  HIV-transmitting partners (n = 15) had a higher median seminal plasma viral load (P < 0.015) and median blood plasma viral load (P < 0.001) than nontransmitting partners (n = 32). Herpes simplex virus 2 serostatus was associated with transmission only when the HIV-infected source partner was herpes simplex virus 2 seropositive and the HIV-exposed partner was not (odds ratio 16, P < 0.03). Adjusting for other factors, HIV transmission was significantly associated with blood plasma viral load (odds ratio 13.4, P < 0.02) but not seminal plasma viral load (odds ratio 0.69, P = not significant).
  
  
  CONCLUSIONS
  Blood and seminal plasma viral load were both associated with human immunodeficiency virus type 1 transmission, but blood plasma viral load was the stronger predictor in this cohort. Herpes simplex virus 2 coinfection was associated with the risk of transmission but not acquisition of human immunodeficiency virus type 1.
authors.affiliation: University of California, San Diego, California, USA. dmbutler@ucsd.edu
authors.forename: David M
authors.initials: DM
authors.lastname: Butler
authors.forename: Davey M
authors.initials: DM
authors.lastname: Smith
authors.forename: Edward R
authors.initials: ER
authors.lastname: Cachay
authors.forename: George K
authors.initials: GK
authors.lastname: Hightower
authors.forename: Charles Thomas
authors.initials: CT
authors.lastname: Nugent
authors.forename: Douglas D
authors.initials: DD
authors.lastname: Richman
authors.forename: Susan J
authors.initials: SJ
authors.lastname: Little
mesh_terms: D000208:Acute Disease; D000328:Adult; D016358:Contact Tracing; D015658:HIV Infections / Q000635:transmission* / Q000821:virology; D015497:HIV-1; D006561:Herpes Simplex / Q000821:virology*; D018258:Herpesvirus 2, Human / Q000235:genetics; D018451:Homosexuality, Male; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D012367:RNA, Viral / Q000032:analysis / Q000097:blood; D012307:Risk Factors; D012661:Semen / Q000821:virology; D019562:Viral Load; D014766:Viremia / Q000635:transmission
chemical_list: D012367:RNA, Viral