[The breeding of Phellinus baumii by protoplast fusion].
OBJECTIVE
To breed good strains by protoplast fusion in Phellinus baumii.


METHODS
5.8S rDNA-ITS sequences, mycelial growth rate, mycelial biomass and exopolysaccharides (EPS) production of 7 strains in different origins were analyzed, respectively. Then the inactivated parent protoplast and the PEG induction were used in the process of protoplast fusion.


RESULTS
The strain P. baumii SA02 and P. baumii SA04 were selected as the parent strains. R002, R003 and R005 had antagonistic effects with parent strains.


CONCLUSIONS
R002, R003 and R005 are fusants of the parent strains.
Zeng
Nian-Kai
NK
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100094, China. niankaiz@163.com
Wang
Qiu-Ying
QY
Su
Ming-Sheng
MS
D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism; D018533:Biomass; D005285:Fermentation; D025282:Mycelium / Q000254:growth & development* / Q000378:metabolism; D011134:Polysaccharides / Q000096:biosynthesis; D011523:Protoplasts
D011134:Polysaccharides