Genetic diversity of avian infectious bronchitis virus isolates in Korea between 2003 and 2006.
Thirty-three field isolates of avian infectious bronchitis virus (IBV) were recovered from commercial chicken flocks in Korea between 2003 and 2006 and were characterized phylogenetically by nucleotide sequence analysis of the IBV S1 gene hyper-variable region. Our phylogenetic analysis revealed that recent field isolates of IBV formed at least three distinct phylogenetic types, including K-I, K-II, and K-III. K-I type IBV consisted of indigenous, 13 IBV isolates which evolved from the Kr-EJ/95 strain and then separated into the lineages of type K-Ia and type K-Ib. K-II type IBV isolates (n = 19) were closely related to nephropathogenic IBV variants from China and Japan. The K-III type isolate (Kr/D064/05), first identified by this study, was closely related to enteric IBV variants from the Chinese strains that cause proventriculitis. Sequence comparisons showed amino acid differences of >27.5% between IBV types. The molecular epidemiologic characteristics of IBV field isolates are briefly discussed.
Lee
Eun-Kyoung
EK
Avian Diseases Division, National Veterinary Research and Quarantine Service, 480 Anyang-6, Anyang, Gyeonggi, 430-824, South Korea.
Jeon
Woo-Jin
WJ
Lee
Youn-Jeong
YJ
Jeong
Ok-Mi
OM
Choi
Jun-Gu
JG
Kwon
Jun-Hun
JH
Choi
Kang-Seuk
KS
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens; D018352:Coronavirus Infections / Q000662:veterinary* / Q000821:virology; D017931:DNA Primers / Q000235:genetics; D005814:Genes, Viral; D014644:Genetic Variation; D001351:Infectious bronchitis virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D007723:Korea; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D017386:Sequence Homology, Amino Acid
D017931:DNA Primers; D012367:RNA, Viral