title: Secretome analysis of Phanerochaete chrysosporium strain CIRM-BRFM41 grown on softwood.
abstract: Proteomic analysis was performed to determine and differentiate the composition of the secretomes of Phanerochaete chrysosporium CIRM-BRFM41, a peroxidase hypersecretory strain grown under ligninolytic conditions and on softwood chips under biopulping conditions. Extracellular proteins from both cultures were analyzed by bidimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry. A total of 37 spots were identified. The secretome in liquid synthetic medium comprised mainly peroxidases, while several wood-degrading enzymes and enzymes involved in fungal metabolism were detected in biopulping cultures on softwood. This prompted an analysis of the impact of secretome modulation in the presence of softwood chips. Biotreated wood was submitted to kraft cooking and chemical bleaching using chlorine dioxide. The fungal pre-treatment led to a significant increase in pulp yield and a better bleachability of the pulp. This bleachability improvement could be explained by the production of specific lignocellulose-degrading enzymes.
authors.affiliation: INRA, UMR1163, Biotechnologie des Champignons Filamenteux, 13000, Marseille, France. holy.ravalason@esil.univmed.fr
authors.forename: Holy
authors.initials: H
authors.lastname: Ravalason
authors.forename: Gwénaël
authors.initials: G
authors.lastname: Jan
authors.forename: Daniel
authors.initials: D
authors.lastname: Mollé
authors.forename: Maryvonne
authors.initials: M
authors.lastname: Pasco
authors.forename: Pedro M
authors.initials: PM
authors.lastname: Coutinho
authors.forename: Catherine
authors.initials: C
authors.lastname: Lapierre
authors.forename: Brigitte
authors.initials: B
authors.lastname: Pollet
authors.forename: Frédérique
authors.initials: F
authors.lastname: Bertaud
authors.forename: Michel
authors.initials: M
authors.lastname: Petit-Conil
authors.forename: Sacha
authors.initials: S
authors.lastname: Grisel
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Sigoillot
authors.forename: Marcel
authors.initials: M
authors.lastname: Asther
authors.forename: Isabelle
authors.initials: I
authors.lastname: Herpoël-Gimbert
mesh_terms: D000595:Amino Acid Sequence; D015180:Electrophoresis, Gel, Two-Dimensional; D005110:Extracellular Space / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D007218:Industrial Microbiology; D008031:Lignin / Q000737:chemistry / Q000378:metabolism*; D008969:Molecular Sequence Data; D020075:Phanerochaete / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport; D040901:Proteomics; D014934:Wood / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D008031:Lignin