title: Microencapsulation of a probiotic bacteria with alginate-gelatin and its properties.
abstract: Lactobacillus casei ATCC 393-loaded microcapsules based on alginate and gelatin had been prepared by extrusion method and the product could increase the cell numbers of L. casei ATCC 393 to be 10(7) CFU g(-1) in the dry state of microcapsules. The microparticles homogeneously distributed with size of 1.1 ± 0.2 mm. Four kinds of microcapsules (S(1), S(2), S(3) and S(4)) exhibited swelling in simulated gastric fluid (SGF) while the beads eroded and disintegrated rapidly in simulated intestinal fluid (SIF). Cells of L. casei ATCC 393 could be continuously released from the microcapsules during simulated gastrointestinal tract (GIT) and the release amounts and speeds in SIF were much higher and faster than that in SGF. Encapsulation in alginate-gelatin microcapsules successfully improved the survival of L. casei ATCC 393 and this approach might be useful in delivery of probiotic cultures as a functional food.
authors.affiliation: College of Marine Life Science, Ocean University of China, Qingdao, P R China.
authors.forename: Xiao Yan
authors.initials: XY
authors.lastname: Li
authors.forename: Xi Guang
authors.initials: XG
authors.lastname: Chen
authors.forename: Dong Su
authors.initials: DS
authors.lastname: Cha
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Park
authors.forename: Cheng Sheng
authors.initials: CS
authors.lastname: Liu
mesh_terms: D000464:Alginates / Q000737:chemistry*; D002214:Capsules; D004339:Drug Compounding / Q000379:methods*; D005766:Gastrointestinal Contents / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007780:Lacticaseibacillus casei / Q000166:cytology*; D010316:Particle Size; D019936:Probiotics / Q000008:administration & dosage*
chemical_list: D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005780:Gelatin