Elevated expression of L-selectin ligand in lymph node-derived human prostate cancer cells correlates with increased tumorigenicity.
Human prostate cancer LNCaP cells including C-33 and C-81 cells were originally derived from the lymph nodes of a patient with metastatic prostate cancer. These two cells were employed for characterization of L-selectin ligand and in vitro tumorigenicity, because they mimic the clinical conditions of early and late-stage human prostate cancer. C-81 cells exhibit higher in vitro migratory and invasive properties as compared with C-33 cells. We find that the L-selectin ligand and mucin glycan-associated MECA-79 epitope were elevated in C-81 cells. An increase of these glycotopes positively correlates with elevated tumorigenicity and expression of key glycosyl- and sulfotransferase genes. These results suggest that modulated expression of selective glycogenes correlates with altered tumorigenicity of cancer cells.
Radhakrishnan
Prakash
P
Department of Biochemistry and Molecular Biology, College of Medicine, 985870, Nebraska Medical Center, University of Nebraska Medical Center, Omaha, NE 68198, USA.
Lin
Ming-Fong
MF
Cheng
Pi-Wan
PW
D000954:Antigens, Surface / Q000096:biosynthesis*; D045744:Cell Line, Tumor; D000939:Epitopes / Q000378:metabolism*; D015973:Gene Expression Regulation, Leukemic; D005964:Glucosyltransferases / Q000378:metabolism; D006801:Humans; D019041:L-Selectin / Q000378:metabolism; D008198:Lymph Nodes / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000096:biosynthesis*; D009077:Mucins / Q000378:metabolism*; D009362:Neoplasm Metastasis; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D015238:Sulfotransferases / Q000378:metabolism
D000954:Antigens, Surface; D000939:Epitopes; C069886:L-selectin counter-receptors; D008565:Membrane Proteins; D009077:Mucins; D019041:L-Selectin; D005964:Glucosyltransferases; D015238:Sulfotransferases