A new strategy to enhance glutathione production by multiple H2O2 induced oxidative stresses in Candida utilis.
Effect of H(2)O(2)-induced oxidative stress on glutathione (GSH) production in Candida utilis was investigated. Based on the results that H(2)O(2) can effectively stimulate GSH accumulation but inhibit cell growth simultaneously, a novel strategy of multiple H(2)O(2) stresses with different concentrations (1 mmol/L at 4h, 2 mmol/L at 8h, and 4 mmol/L at 12h) were developed to maximize GSH production. As a result, a maximal GSH yield of 218 mg/L was achieved and a corresponding intracellular GSH content was 2.15%, which were 54.6% and 58.1% higher than the control. By further applying this strategy to 7 L fermentor, GSH yield and intracellular GSH content were 328 mg/L and 2.30%. Moreover, increased activities of catalase (CAT) and GSH reductase (GR) indicated that GSH and CAT were directly involved in protecting cell against oxidative stress by H(2)O(2).
Liang
Guobin
G
School of Biotechnology, Jiangnan University, Wuxi, China.
Liao
Xianyan
X
Du
Guocheng
G
Chen
Jian
J
D002175:Candida / Q000166:cytology / Q000187:drug effects / Q000502:physiology*; D002374:Catalase / Q000378:metabolism*; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005978:Glutathione / Q000378:metabolism*; D005980:Glutathione Reductase / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000008:administration & dosage*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology
D006861:Hydrogen Peroxide; D002374:Catalase; D005980:Glutathione Reductase; D005978:Glutathione