[Blocking effect of phytic acid on cell proliferation in human gastric carcinoma].
OBJECTIVE
To explore the bcl-2 and the bax protein expression, the effect and possible mechanism of phytic acid (IP6) on cell proliferation in human gastric carcinoma.


METHODS
The inhibiting action of IP6 on human gastric carcinoma was examed by MTT assay. The morphological changes of SGC-7901 cells exposed to IP6 was examined by reverse discrepancy microscope. The apoptosis of SGC-7901 cells treated with IP6 was observed by single cell gel electrophoresis. The bax and bcl-2 protein expressions were detected by Western blotting method.


RESULTS
MTT assay indicated that the growth of SGC-7901 cells were inhibited by IP6 in dose and time dependent manners. The morphological observation by reverse discrepancy microscope indicated that the growth of cells exposed to IP6 were not well. The DNA damage rates of SGC-7901 cells treated with IP6 were more higher than those of control groups in dose and time dependent manners. The bcl-2 protein expressions treated with IP6 were reduced, and the bax protein expressions treated with IP6 were more than those of control groups in dose and time dependent manners.


CONCLUSIONS
The proliferation of gastric carcinoma SGC-7901 cells inhibitited by IP6 could be associated with apoptosis of gene bax and bcl-2.
Wang
Lu
L
Department of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China.
Yang
Zhiping
Z
Cui
Hongbin
H
D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D010833:Phytic Acid / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000473:pathology*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
D000970:Antineoplastic Agents; C494826:BAX protein, human; D019253:Proto-Oncogene Proteins c-bcl-2; D051028:bcl-2-Associated X Protein; D010833:Phytic Acid