title: Isolation of human multipotent neural progenitors from adult filum terminale.
abstract: Stem cells have been isolated from several CNS regions, including the spinal cord. However, the terminal end of the spinal cord, filum terminale, has been referred to as a fibrovascular tag without neurogenic potential and of no clinical significance. Recently, we were fortunate to acquire some samples of this tissue. We show for the first time that progenitor cells exhibiting the hallmarks of stem cells can be isolated from adult human filum terminale (FTNPs). More specifically, FTNPs self-renew and proliferate to form neurospheres, and exhibit tripotent differentiation into neurons, astrocytes, and oligodendrocytes. Equally important, FTNPs develop the electrophysiological profile of neurons and glia. Whole-cell patch-clamp recordings show beta-III-tubulin(+) neurons exhibiting overshooting action potentials, displaying both the fast inactivating TTX-sensitive sodium current as well as 4-AP and TEA sensitive potassium currents. To assess potency in vivo, FTNPs were transplanted into the posterior periventricular region of control or ischemic rat brains. Despite a vigorous immune response against the xenograft, FTNPs survived and were found not only in the graft area but had also migrated to the lesioned CA1 region. Notwithstanding the immune response, FTNPs differentiated into astrocytes, but no neuronal differentiation was observed in the transplant milieu tested. However, neuronal differentiation in vivo cannot be ruled out and assessment of the conditions necessary to promote neurogenesis in vivo requires more research. Significantly, no tumor formation or aberrant cell morphology was seen in or adjacent to the graft area. Thus, filum terminale provides a novel source of adult human neural progenitor cells that develop into functional neurons with possible clinical applications.
authors.affiliation: Vilhelm Magnus Laboratory for Neurosurgical Research, Institute for Surgical Research, University of Oslo, Oslo, Norway. mercy.varghese@medisin.uio.no
authors.forename: Mercy
authors.initials: M
authors.lastname: Varghese
authors.forename: Havard
authors.initials: H
authors.lastname: Olstorn
authors.forename: Jon
authors.initials: J
authors.lastname: Berg-Johnsen
authors.forename: Morten C
authors.initials: MC
authors.lastname: Moe
authors.forename: Wayne
authors.initials: W
authors.lastname: Murrell
authors.forename: Iver A
authors.initials: IA
authors.lastname: Langmoen
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000328:Adult; D000818:Animals; D001706:Biopsy; D002420:Cauda Equina / Q000166:cytology*; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation; D002469:Cell Separation / Q000379:methods*; D002478:Cells, Cultured; D002648:Child; D006801:Humans; D008564:Membrane Potentials / Q000502:physiology; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000166:cytology* / Q000502:physiology*; D009474:Neurons / Q000166:cytology / Q000502:physiology*; D018408:Patch-Clamp Techniques; D051381:Rats; D033581:Stem Cell Transplantation; D055815:Young Adult