[Preparation of chitosan microparticles and study of their interaction with interferon].
Chitosan with viscosity average molecular weights of 340, 281, 199, 137, and 42 kDa was used to prepare microparticles by precipitation coacervation. Acid and enzymatic hydrolysis yielded chitosan samples with a deacetylation degree of 0.85 +/- 0.03. The chitosan microparticles were 0.85-1.7 microm in size and had a zeta-potential of 30.7-38 +/- 0.1 mV. Testing the microparticles for toxicity showed that they caused no death of animals. Interaction of recombinant alpha-2 interferon with the microparticles was studied by sorption from solutions. The maximum adsorption efficiency of interferon was 88% and the capacity of microparticles was 11.8-12.7 microg/mg.
Il'ina
A V
AV
Gubaĭdullina
A A
AA
Melent'ev
A I
AI
Varlamov
V P
VP
D000327:Adsorption; D000818:Animals; D048271:Chitosan / Q000737:chemistry* / Q000633:toxicity; D016503:Drug Delivery Systems; D007370:Interferon Type I / Q000008:administration & dosage / Q000737:chemistry*; D008297:Male; D051379:Mice; D008863:Microspheres; D010316:Particle Size; D011994:Recombinant Proteins
D007370:Interferon Type I; D011994:Recombinant Proteins; D048271:Chitosan