title: Overexpression of a fatty acid amide hydrolase compromises innate immunity in Arabidopsis.
abstract: N-acylethanolamines are a group of lipid mediators that accumulate under a variety of neurological and pathological conditions in mammals. N-acylethanolamine signaling is terminated by the action of diverse hydrolases, among which fatty acid amide hydrolase (FAAH) has been well characterized. Here, we show that transgenic Arabidopsis lines overexpressing an AtFAAH are more susceptible to the bacterial pathogens Pseudomonas syringae pv. tomato and P. syringae pv. maculicola. AtFAAH overexpressors also were highly susceptible to non-host pathogens P. syringae pv. syringae and P. syringae pv. tabaci. AtFAAH overexpressors had lower amounts of jasmonic acid, abscisic acid and both free and conjugated salicylic acid (SA), compared with the wild-type. Gene expression studies revealed that transcripts of a number of plant defense genes, as well as genes involved in SA biosynthesis and signaling, were lower in AtFAAH overexpressors than wild-type plants. Our data suggest that FAAH overexpression alters phytohormone accumulation and signaling which in turn compromises innate immunity to bacterial pathogens.
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Kang
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Yuh-Shuh
authors.initials: YS
authors.lastname: Wang
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Srinivasa Rao
authors.initials: SR
authors.lastname: Uppalapati
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Keri
authors.initials: K
authors.lastname: Wang
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Vatsala
authors.initials: V
authors.lastname: Vadapalli
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Barney J
authors.initials: BJ
authors.lastname: Venables
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Kent D
authors.initials: KD
authors.lastname: Chapman
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Elison B
authors.initials: EB
authors.lastname: Blancaflor
authors.affiliation: Plant Biology Division, Samuel Roberts Noble Foundation, Ardmore, OK, USA, andCenter for Plant Lipid Research, Department of Biological Sciences, University of North Texas, Denton, TX, USA.
authors.forename: Kirankumar S
authors.initials: KS
authors.lastname: Mysore
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D000581:Amidohydrolases / Q000378:metabolism*; D017360:Arabidopsis / Q000201:enzymology* / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D003517:Cyclopentanes / Q000378:metabolism; D004983:Ethanolamines / Q000378:metabolism*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007113:Immunity, Innate; D020411:Oligonucleotide Array Sequence Analysis; D054883:Oxylipins / Q000378:metabolism; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010937:Plant Growth Regulators / Q000378:metabolism; D018515:Plant Leaves / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D030821:Plants, Genetically Modified / Q000201:enzymology / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D044224:Pseudomonas syringae / Q000472:pathogenicity; D018749:RNA, Plant / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D020156:Salicylic Acid / Q000378:metabolism; D000099252:Fatty Acid Amide Hydrolases
chemical_list: D000040:Abscisic Acid; D000581:Amidohydrolases; D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D004983:Ethanolamines; D054883:Oxylipins; D010937:Plant Growth Regulators; D018749:RNA, Plant; D020156:Salicylic Acid; D000099252:Fatty Acid Amide Hydrolases; C022203:N-acylethanolamines; C011006:jasmonic acid