Enzymatic inhibitory activity and trypanocidal effects of extracts and compounds from Siphoneugena densiflora O. Berg and Vitex polygama Cham.
Hexanic, methanolic, and hydroalcoholic extracts, and 34 isolated compounds from Vitex polygama Cham. (Lamiaceae, formely Verbenaceae) and Siphoneugena densiflora O. Berg (Myrtaceae) were screened for their trypanocidal effects on bloodstream forms of Trypanosoma cruzi and T brucei, as well as for their enzymatic inhibitory activities on glycosomal glyceraldehyde-3-phosphate dehydrogenase (gGAPDH) and trypanothione reductase (TR) enzymes from T cruzi and adeninephosphoribosyl transferase (APRT) enzyme from Leishmania tarentolae. In general, polar extracts displayed strong effects and some of the tested compounds have shown good results in comparison to positive controls of the bioassays.
Gallo
Margareth B C
MB
Departamento de Química, Universidade Federal de São Carlos, CP 676, 13565-905, São Carlos-SP, Brazil. margareth.gallo@gmail.com
Marques
Anna Sylvia F
AS
Vieira
Paulo C
PC
da Silva
Maria Fátima das G F
MF
Fernandes
João B
JB
Silva
Márcio
M
Guido
Rafael V
RV
Oliva
Glaucius
G
Thiemann
Otávio H
OH
Albuquerque
Sérgio
S
Fairlamb
Alan H
AH
D000818:Animals; D000953:Antigens, Protozoan / Q000187:drug effects; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000037:antagonists & inhibitors; D007202:Indicators and Reagents; D010936:Plant Extracts / Q000302:isolation & purification* / Q000494:pharmacology; D012997:Solvents; D027842:Syzygium / Q000737:chemistry*; D014344:Trypanocidal Agents / Q000302:isolation & purification* / Q000494:pharmacology; D014349:Trypanosoma cruzi / Q000187:drug effects* / Q000201:enzymology; D020950:Vitex / Q000737:chemistry*
D000953:Antigens, Protozoan; D007202:Indicators and Reagents; D010936:Plant Extracts; D012997:Solvents; D014344:Trypanocidal Agents; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases