title: [An improved replica plating method and its application to the high-throughput selection of transformants].
abstract: OBJECTIVE
  To make a simpler and highly efficient replica plating tool to avoid the limitations of the traditional method.
  
  
  METHODS
  A number of bamboo-toothpicks was sheaved to form a bundle with diameter close to the plate diameter. The sheaved bamboo-toothpicks were used for replica-plating.
  
  
  RESULTS
  This replica plating tool was successfully used for the screening of Saccahromyces cerevisiae transformants.
  
  
  CONCLUSIONS
  Compared with the traditional tool, this tool was simpler and highly efficient.
authors.affiliation: State Key Laboratory for Biohazard Control, College of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China. xuminjun1974@yahoo.com.cn
authors.forename: Minjun
authors.initials: M
authors.lastname: Xu
authors.forename: Xiaoqun
authors.initials: X
authors.lastname: Tian
authors.forename: Shining
authors.initials: S
authors.lastname: Zhou
mesh_terms: D003365:Costs and Cost Analysis; D005818:Genetic Engineering / Q000295:instrumentation*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000302:isolation & purification*; D013997:Time Factors; D014170:Transformation, Genetic