[Effect of guanxin no. 2 on gene variant expression profile in rats after myocardial ischemia].
OBJECTIVE
To study the effect of Guanxin No. 2 (GX2) on gene variant expression profile in rats after myocardial infarction (MI).


METHODS
Six SD rats were equally randomized into the sham operated group, the model group and the GX2 group, and they received gastric perfusion with water and GX2 (10 g/kg) respectively. MI model was established by ligating the left-anterior descending branch of coronary artery after 10 days of perfusion, and rats' myocardial tissue in the junction zone was assessed 24 h later for gene chip detection with DNA microarray. Then a cluster analysis was conducted, and the different expressions of key genes were verified by real-time reverse transcription-polymerase chain reaction.


RESULTS
The up-regulating gene expressions in myocardial tissue in the junction zone increased after ischemia. After GX2 intervention, the up- or down-regulating gene expressions, especially the 2 genes, all-trans-13,14-dihydroretinol saturase (AF465614) and similar to expressed sequence AW413625 (AA799328) decreased significantly. In the common genes, more genes involving activity of signal transducer presented in the model group and the GX2 group and those in the latter showed a certain specificity.


CONCLUSIONS
GX2 could improve the characteristics of variant gene expression profile in MI rats to a certain extent.
Yu
Cheng-Yao
CY
Department of TCM, Beijing Shijitan Hospital, Beijing.
Gao
Hui-Li
HL
Liu
Zhen
Z
D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D015870:Gene Expression / Q000187:drug effects*; D020869:Gene Expression Profiling; D017202:Myocardial Ischemia / Q000235:genetics / Q000378:metabolism*; D009206:Myocardium / Q000378:metabolism*; D044925:Oxidoreductases Acting on CH-CH Group Donors / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; C539574:guanxin No. 2; D044925:Oxidoreductases Acting on CH-CH Group Donors