[Effects of Chinese herb compound Naoyikang on expression of choline acetyltransferase in brain of rats with Alzheimer's disease].
OBJECTIVE
To observe the effects of Naoyikang (NYK) on expression of choline acetyltransferase (ChAT) in brain of rats with Alzheimer' s disease (AD).


METHODS
Bilateral infusions of Ibotenic acid (IBO) into nucleus basalis of Meynert (NBM) using hamilton syringe and stereotaxic apparatus were adopted to establish the rat model of AD. After intragastrically administrated with different solution for 28 days, immunohistochemistry and Western-blot were adopted to study the expression of ChAT in frontal cortex of AD rats.


RESULTS
NYK could improve the morphology and increase the number of ChAT immunoreactive neurons, and significantly promote ChAT protein expression.


CONCLUSIONS
NYK may be able to increase the synthesis of acetylcholine (ACh) through elevating the expression of ChAT protein, thus improving the level of brain ACh so as to protect central cholinergic neurons.
Geng
Jin-Song
JS
Medical School of Nantong University, Nantong 226001, China.
Zhou
Ai-Ling
AL
Shi
Hai-Yan
HY
Hu
Ya-Er
YE
Mao
Jia-Hui
JH
Zhu
Yan
Y
D000544:Alzheimer Disease / Q000201:enzymology*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000201:enzymology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
D004365:Drugs, Chinese Herbal; D002795:Choline O-Acetyltransferase