Mannose-specific interaction of Lactobacillus plantarum with porcine jejunal epithelium.
Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and msa-mutant strain had been applied. In contrast to the mutant strains, the L. plantarum wild-type strain tended to induce a decrease in jejunal net fluid absorption compared with control conditions. Furthermore, after 8 h of perfusion expression of the host gene encoding pancreatitis-associated protein, a protein with proposed bactericidal properties, was found to be upregulated by the wild-type strain only. These observations suggest a role of Msa in the induction of host responses in the pig intestine.
Gross
Gabriele
G
Animal Breeding and Genomics Centre, Animal Sciences Group of Wageningen UR, Lelystad, The Netherlands.
van der Meulen
Jan
J
Snel
Johannes
J
van der Meer
Roelof
R
Kleerebezem
Michiel
M
Niewold
Theo A
TA
Hulst
Marcel M
MM
Smits
Mari A
MA
D018829:Adhesins, Bacterial / Q000235:genetics / Q000502:physiology; D019881:Aminoacyltransferases / Q000235:genetics / Q000502:physiology; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000502:physiology; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000382:microbiology*; D007583:Jejunum / Q000382:microbiology* / Q000502:physiology; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000502:physiology*; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism; D008297:Male; D008358:Mannose / Q000378:metabolism*; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D000073718:Pancreatitis-Associated Proteins; D019936:Probiotics; D013552:Swine
D018829:Adhesins, Bacterial; D000951:Antigens, Neoplasm; D001426:Bacterial Proteins; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; C062777:adhesin, Lactobacillus; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases; D008358:Mannose