title: Cloning and functional characterization of chicken interleukin-17D.
abstract: The chicken interleukin-17D was cloned from a testis cDNA library prepared from the Korean native chicken. The full-length chicken IL-17D (chIL-17D) cDNA consisted of a 348 nucleotide sequence encoding an open reading frame of 116 amino acids with a predicted molecular mass of 13.3kDa. Comparison of the deduced amino acid sequence of chIL-17D with homologous proteins from human, mouse and opossum revealed 64%, 53% and 76% identity, respectively, including six conserved cysteine residues present in the mammalian polypeptides. The chIL-17D gene transcript was expressed in a wide range of tissues, and highest levels were in pancreas, thymus and lung. Following Eimeria maxima infection, levels of the chIL-17D mRNA were up-regulated in the intestinal jejunum, bursa, lung, and spleen but decreased in the thymus. Infected chickens also expressed greater levels of chIL-17D mRNA in CD4(+), CD8(+) and TCR1(+) intestinal intraepithelial lymphocytes while decreased expression was seen in TCR2(+) cells. Treatment of CHCC-OU2 fibroblasts with chIL-17D recombinant protein induced the expression of IL-6 and IL-8. Collectively, these results suggest that chL-17D has structural and functional similarities to mammalian IL-17Ds and that it plays an important role in local gut innate immune responses during experimental coccidiosis.
authors.affiliation: Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Building 1040, BARC-East, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
authors.forename: Yeong Ho
authors.initials: YH
authors.lastname: Hong
authors.forename: Hyun S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: Dong Woon
authors.initials: DW
authors.lastname: Park
authors.forename: Sung Hyen
authors.initials: SH
authors.lastname: Lee
authors.forename: Jae Yong
authors.initials: JY
authors.lastname: Han
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Shin
authors.forename: Myeung Sun
authors.initials: MS
authors.lastname: Park
authors.forename: Jin-Kyoo
authors.initials: JK
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004539:Eimeria; D020381:Interleukin-17 / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D016053:RNA, Protozoan / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D016054:DNA, Protozoan; D020381:Interleukin-17; D012333:RNA, Messenger; D016053:RNA, Protozoan