Transient increase in the transcript levels of gamma-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls.
By hypergravity treatment, the percentage of cells with transverse microtubules was decreased, while that with longitudinal microtubules was increased in azuki bean (Vigna angularis) epicotyls. The expression of genes encoding gamma-tubulin complex (VaTUG and VaGCP3) was increased transiently in response to changes in the gravitational conditions. Lanthanum and gadolinium ions, potential blockers of mechanosensitive calcium ion-permeable channels (mechanoreceptors), nullified reorientation of microtubules as well as up-regulation of expression of VaTUG and VaGCP3 by hypergravity. These results suggest that mechanoreceptors may perceive the gravity signal, which leads to a transient increase in the transcript levels of gamma-tubulin complex genes and reorientation of cortical microtubules.
Soga
Kouichi
K
Department of Biology and Geosciences, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka, 558-8585, Japan. soga@sci.osaka-cu.ac.jp.
Kotake
Toshihisa
T
Wakabayashi
Kazuyuki
K
Kamisaka
Seiichiro
S
Hoson
Takayuki
T
D002498:Centrifugation; D007887:Fabaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005682:Gadolinium; D015870:Gene Expression; D018522:Gravitropism; D007811:Lanthanum; D008870:Microtubules / Q000502:physiology*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*
D014404:Tubulin; D007811:Lanthanum; D005682:Gadolinium