Appearance of novel G-bacteria belonging to acidobacteria in a dairy wastewater treatment plant.
A dairy wastewater treatment plant operates a sequencing batch reactor (SBR) and stimulates enhanced biological phosphorus removal (EBPR) process with alternating anaerobic and aerobic cycles. Occasionally, the plant suffers from a high suspended solids problem in the supernatant. Interestingly, the occurrence of high suspended solids coincided with times when the EBPR process failed to remove phosphorus. To find out if there was a relationship between the EBPR failure and the high suspended solids problem, effluent samples were collected from the site during the period of poor phosphorus removal and examined microscopically. It was found that cocci-shaped bacteria (3-4 microm in diameter) were abundant in the effluent samples and they were clustered in tetrads. These were believed to be G-bacteria and results of both Gram and Neisser staining tests were negative, suggesting that they had few intracellular polyphosphate granules. Using polymerase chain reaction (PCR), cloning and deoxyribonucleic acid (DNA) sequence analysis, the phylogenetic information of in situ G-bacteria was obtained. It was found that all of the recovered clones were clustered in the phylum of Acidobacteria.
Ahn
C H
CH
Department of Civil and Environmental Engineering, University of Wisconsin, Madison, Wisconsin, 53706, USA.
Park
H-D
HD
Lee
Y O
YO
Park
J K
JK
D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D003612:Dairying; D008855:Microscopy, Electron, Scanning; D008858:Microscopy, Phase-Contrast; D010758:Phosphorus / Q000378:metabolism; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D020560:Proteobacteria / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification* / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000737:chemistry / Q000235:genetics; D014865:Waste Disposal, Fluid / Q000379:methods*; D014874:Water Pollutants, Chemical / Q000378:metabolism
D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D010758:Phosphorus