[The application of lentiviral vectors for tissue-specific gene manipulations].
The trophectoderm (TE) ofblastocysts, the first epithelium established in mammalian development, 1) plays signaling, supportive, and patterning functions during pre-implantation development, 2) ensures embryo implantation into the uterine wall, and 3) gives rise to extra-embryonic tissues essential for embryo patterning and growth after implantation. We show that mouse TE, itself permissive to lentiviral (LV) infection, represents a robust non-permeable physical barrier to the virus particles, thereby shielding the cells of the inner cell mass (ICM) from viral infection. This LV feature will allow modulations of gene expression in a lineage-specific manner, thus having significant applications in mouse functional genetics.
Malashicheva
A B
AB
Kanzler
B
B
Tolkunova
E N
EN
Trono
D
D
Tomilin
A N
AN
D000818:Animals; D001755:Blastocyst / Q000821:virology; D002460:Cell Line; D019070:Cell Lineage; D004622:Embryo, Mammalian / Q000821:virology; D018014:Gene Transfer Techniques; D005822:Genetic Vectors / Q000378:metabolism; D006801:Humans; D016086:Lentivirus / Q000378:metabolism; D051379:Mice