title: Emdogain stimulates matrix degradation by osteoblasts.
abstract: Emdogain has been used clinically for periodontal regeneration, although the underlying molecular mechanisms are not clear at present. In this study, we hypothesized that Emdogain stimulated degradation of type I collagen via osteoblasts. We showed that Emdogain enhanced cell-mediated degradation of type I collagen in an MMP-dependent manner. Although MG-63 cells spontaneously produced a zymogen form of MMP-1, treatment with Emdogain significantly induced the generation of the active form of this enzyme. We demonstrated that MMP-3 was produced from MG63 cells in response to Emdogain in a MEK1/2-dependent manner. Concomitantly, blocking of MEK1/2 activation by U0126 significantly inhibited the generation of the active form of MMP-1 without affecting the total production of this collagenase. These results suggest that Emdogain facilitates tissue regeneration through the activation of the collagenase, MMP-1, that degrades matrix proteins in bone tissue microenvironments.
authors.affiliation: Departments of Biochemistry, Osaka Dental University, Osaka, Japan. goda@cc.osaka-dent.ac.jp
authors.forename: S
authors.initials: S
authors.lastname: Goda
authors.forename: H
authors.initials: H
authors.lastname: Inoue
authors.forename: Y
authors.initials: Y
authors.lastname: Kaneshita
authors.forename: Y
authors.initials: Y
authors.lastname: Nagano
authors.forename: T
authors.initials: T
authors.lastname: Ikeo
authors.forename: Y T
authors.initials: YT
authors.lastname: Ikeo
authors.forename: J
authors.initials: J
authors.lastname: Iida
authors.forename: N
authors.initials: N
authors.lastname: Domae
mesh_terms: D001861:Bone Regeneration / Q000502:physiology; D002460:Cell Line; D024042:Collagen Type I / Q000378:metabolism*; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D048369:MAP Kinase Kinase 1 / Q000378:metabolism; D048730:MAP Kinase Kinase Kinase 2 / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism*; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D024042:Collagen Type I; D003746:Dental Enamel Proteins; C109069:enamel matrix proteins; D048730:MAP Kinase Kinase Kinase 2; D048369:MAP Kinase Kinase 1; D020784:Matrix Metalloproteinase 8; D020781:Matrix Metalloproteinase 1