Production of butyric acid from glucose and xylose with immobilized cells of Clostridium tyrobutyricum in a fibrous-bed bioreactor.
Butyric acid has many applications in chemical, food, and pharmaceutical industries. In the present study, Clostridium tyrobutyricum ATCC 25755 was immobilized in a fibrous-bed bioreactor to evaluate the performance of butyrate production from glucose and xylose. The results showed that the final concentration and yield of butyric acid were 13.70 and 0.46 g g(-1), respectively, in batch fermentation when 30 g L(-1) glucose was introduced into the bioreactor. Furthermore, high concentration 10.10 g L(-1) and yield 0.40 g g(-1) of butyric acid were obtained with 25 g L(-1) xylose as the carbon source. The immobilized cells of C. tyrobutyricum ensured similar productivity and yield from repeated batch fermentation. In the fed-batch fermentation, the final concentration of butyric acid was further improved to 24.88 g L(-1) with one suitable glucose feeding in the fibrous-bed bioreactor. C. tyrobutyricum immobilized in the fibrous-bed bioreactor would provide an economically viable fermentation process to convert the reducing sugars derived from plant biomass into the final bulk chemical (butyric acid).
Jiang
Ling
L
South China University of Technology, Guangzhou, China.
Wang
Jufang
J
Liang
Shizhong
S
Wang
Xiaoning
X
Cen
Peilin
P
Xu
Zhinan
Z
D019149:Bioreactors / Q000382:microbiology*; D020148:Butyric Acid / Q000378:metabolism*; D018914:Cells, Immobilized / Q000378:metabolism; D048014:Clostridium tyrobutyricum / Q000378:metabolism*; D005285:Fermentation; D005947:Glucose / Q000378:metabolism*; D014994:Xylose / Q000378:metabolism*
D020148:Butyric Acid; D014994:Xylose; D005947:Glucose