Subunit structure of glycinin and its molecular species based on RP-HPLC, gel electrophoresis and SEC studies.
Glycinin and its molecular species (glycinin I and glycinin II) were separated and isolated. The number and kind of subunits of glycinin, glycinin I and glycinin II were determined. Studies were carried out under different experimental conditions using slab gel electrophoresis, size-exclusion chromatography and reversed-phase high performance liquid chromatography. Gel electrophoresis was done using both continuous and discontinuous system and under varying concentrations of resolving gel. In addition, the subunits were separated by reversed phase using gradient program. Glycinin and glycinin II were found to have 12 subunits each while glycinin I showed six subunits. Molecular weight and weight ratio in each case were also determined.
Bhushan
Ravi
R
Department of Chemistry, Indian Institute of Technology Roorkee, Roorkee 247667, India. rbushfcy@iitr.ernet.in
Arya
Upasana
U
D002850:Chromatography, Gel / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004591:Electrophoresis, Polyacrylamide Gel / Q000379:methods*; D005916:Globulins / Q000737:chemistry*; D021122:Protein Subunits / Q000737:chemistry; D030262:Soybean Proteins / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry
D005916:Globulins; D021122:Protein Subunits; D030262:Soybean Proteins; C011474:glycinin