The study of improved controlled release of vincristine sulfate from collagen-chitosan complex film.
A water-in-oil-in-oil double-emulsion solvent/evaporation method was used to prepare vincristine sulfate (VCR) loaded poly(lactide-co-glycolide) microspheres, and then VCR microspheres were mixed with collagen and (or) chitosan swelling solution and lyophilized to form polymeric films. The films were cross-linked by 0.3% glutaraldehyde (GA). Encapsulation efficiency and release kinetics of VCR microspheres were determined, as well as release kinetics and in vitro degradation of the film. The rate of VCR release from the film submerged in PBS (pH 6.8) and the content were measured by high-performance liquid chromatography (HPLC). The physichemical properties of the film, such as surface morphology, mechanical function, and differential scanning calorimetry, were also measured. VCR was released from the film in a prolonged period and the initial burst release of the film was less significant. In the degradation experiment, the film containing chitosan degraded more slowly than that without chitosan. The films comprising collagen and chitosan could achieve the release kinectics of a relatively constant release. It has a promising future.
Chen
Hongli
H
The Key Laboratory of Biomedical Material of Tianjin, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, People's Republic of China.
Chen
Han
H
Liu
Lingrong
L
Yuan
Ping
P
Zhang
Qiqing
Q
D020341:Absorbable Implants; D000704:Analysis of Variance; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000737:chemistry / Q000493:pharmacokinetics; D002152:Calorimetry, Differential Scanning; D048271:Chitosan / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry* / Q000378:metabolism; D017364:Collagenases / Q000378:metabolism; D004343:Drug Implants / Q000138:chemical synthesis* / Q000493:pharmacokinetics; D004655:Emulsions; D019344:Lactic Acid / Q000737:chemistry; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D016062:Porosity; D013499:Surface Properties; D013718:Tensile Strength; D013997:Time Factors; D014750:Vincristine / Q000008:administration & dosage / Q000737:chemistry* / Q000493:pharmacokinetics
D000972:Antineoplastic Agents, Phytogenic; D004343:Drug Implants; D004655:Emulsions; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D014750:Vincristine; D003094:Collagen; D048271:Chitosan; D017364:Collagenases; C417249:collagenase 1