Actin restricts FcepsilonRI diffusion and facilitates antigen-induced receptor immobilization.
The actin cytoskeleton has been implicated in restricting diffusion of plasma membrane components. Here, simultaneous observations of quantum dot-labelled FcepsilonRI motion and GFP-tagged actin dynamics provide direct evidence that actin filament bundles define micron-sized domains that confine mobile receptors. Dynamic reorganization of actin structures occurs over seconds, making the location and dimensions of actin-defined domains time-dependent. Multiple FcepsilonRI often maintain extended close proximity without detectable correlated motion, suggesting that they are co-confined within membrane domains. FcepsilonRI signalling is activated by crosslinking with multivalent antigen. We show that receptors become immobilized within seconds of crosslinking. Disruption of the actin cytoskeleton results in delayed immobilization kinetics and increased diffusion of crosslinked clusters. These results implicate actin in membrane partitioning that not only restricts diffusion of membrane proteins, but also dynamically influences their long-range mobility, sequestration and response to ligand binding.
Andrews
Nicholas L
NL
Department of Pathology and Cancer Research and Treatment Center, University of New Mexico, Albuquerque, New Mexico 87131, USA.
Lidke
Keith A
KA
Pfeiffer
Janet R
JR
Burns
Alan R
AR
Wilson
Bridget S
BS
Oliver
Janet M
JM
Lidke
Diane S
DS
D008841:Actin Cytoskeleton; D000199:Actins / Q000502:physiology*; D000818:Animals; D000941:Antigens / Q000378:metabolism; D003599:Cytoskeleton; D004058:Diffusion; D007073:Immunoglobulin E / Q000378:metabolism*; D007700:Kinetics; D009038:Motion; D051381:Rats; D017455:Receptors, IgE / Q000378:metabolism*; D015398:Signal Transduction
D000199:Actins; D000941:Antigens; D017455:Receptors, IgE; D007073:Immunoglobulin E