title: Mutational analysis of the -10 region from the Mycobacterium tuberculosis lipF promoter.
abstract: The promoter driving expression of the Mycobacterium tuberculosis gene lipF (Rv3487c) had previously been identified as being upregulated by acidic stress. Subsequently a 59 base pair (bp) acid inducible minimal promoter region was isolated in which a putative -10 region was identified. In this study we use mutational analysis to investigate the -10 region of the lipF promoter. Mutations within this region lead to a dramatic decrease of promoter activity, while a mutation outside of this region does not affect promoter activity.
authors.affiliation: Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA.
authors.forename: Michelle
authors.initials: M
authors.lastname: Gonzales
authors.forename: Beatrice
authors.initials: B
authors.lastname: Saviola
mesh_terms: D004252:DNA Mutational Analysis / Q000379:methods*; D004950:Esterases / Q000235:genetics*; D015964:Gene Expression Regulation, Bacterial; D005798:Genes, Bacterial; D009169:Mycobacterium tuberculosis / Q000201:enzymology* / Q000235:genetics; D011401:Promoter Regions, Genetic
chemical_list: D004950:Esterases