title: In vitro cytotoxic activity of Salsola oppositifolia Desf. (Amaranthaceae) in a panel of tumour cell lines.
abstract: The aim of the present study was to evaluate for the first time the in vitro cytotoxic activity of fractions and isolated flavonols from Salsola oppositifolia Desf. (Amaranthaceae). The n-hexane fraction demonstrated an effective cytotoxic activity on the large lung carcinoma and amelanotic melanoma cell lines with IC50 values of 19.1 microg/ml and 24.4 microg/ml, respectively. Also the dichloromethane fraction exhibited cytotoxic activity against COR-L23 (IC50 30.4 microg/ml) and C32 (IC50 33.2 microg/ml) cells, while the EtOAc fraction demonstrated a selective cytotoxic activity against MCF-7 cells (IC50 67.9 microg/ml). The major active constituents of this fraction were isorhamnetin-3-O-glucoside (1) and isorhamnetin-3-O-rutinoside (2), which showed an interesting activity against the cell line MCF-7 with IC50 values of 18.2 and 25.2 microg/ml, respectively. Compound 2 exhibited a strong activity against the hormone-dependent prostate carcinoma LNCaP cell line with an IC50 of 20.5 microg/ml. Constituents of S. oppositifolia were identified by GC-MS and NMR analyses.
authors.affiliation: Department of Pharmaceutical Sciences, Faculty of Pharmacy, Nutrition and Health Sciences, University of Calabria, I-87030 Rende (CS), Italy. tundis@unical.it
authors.forename: Rosa
authors.initials: R
authors.lastname: Tundis
authors.forename: Monica R
authors.initials: MR
authors.lastname: Loizzo
authors.forename: Marco
authors.initials: M
authors.lastname: Bonesi
authors.forename: Federica
authors.initials: F
authors.lastname: Menichini
authors.forename: Giancarlo A
authors.initials: GA
authors.lastname: Statti
authors.forename: Francesco
authors.initials: F
authors.lastname: Menichini
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry; D000970:Antineoplastic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D001943:Breast Neoplasms; D045744:Cell Line, Tumor; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D006801:Humans; D007680:Kidney Neoplasms; D008545:Melanoma; D000432:Methanol; D017239:Paclitaxel / Q000302:isolation & purification / Q000494:pharmacology; D010636:Phenols / Q000302:isolation & purification / Q000494:pharmacology; D035261:Plant Components, Aerial / Q000737:chemistry*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology; D031248:Salsola / Q000737:chemistry*; D014747:Vinblastine / Q000302:isolation & purification / Q000494:pharmacology
chemical_list: D000970:Antineoplastic Agents; D010636:Phenols; D010936:Plant Extracts; D014747:Vinblastine; D017239:Paclitaxel; D000432:Methanol