Kinetic assay for the determination of the oxidative stress biomarker, advanced oxidation protein products (AOPP) in the human blood plasma.
A number of human diseases and pathological conditions were found to be associated with increased oxidative stress. In the literature several techniques are available for the assessment of oxidative stress, but most of them are not applicable for a routine medical laboratory due to the complex methodology and/or financial reasons. We report here on a simple, inexpensive, kinetic assay for the determination of the oxidative stress biomarker, advanced oxidation protein products (AOPP) in the human blood plasma.


METHODS
This study involved 70 patients (47M/23F; mean age: 64.6 y; range: 16-85) admitted to our Department with a wide range of cardiovascular and peripheral vascular diseases. Three critically ill patients were assigned for monitoring purposes. Plasma AOPP were simultaneously determined using an end-point assay as reference method and by a kinetic method developed in our laboratory. Plasma fibrinogen concentration was measured according to the Clauss method.


RESULTS
There was a highly significant correlation (r2 = 0.588; p < 0.0001) between AOPP concentration (reference method) and AOPP reactivity (kinetic method). Both AOPP concentration and AOPP reactivity also significantly correlated with plasma fibrinogen concentration (r2 = 0.780; p < 0.0001; r2 = 0.564; p < 0.0001). The three representative cases presented appear to support the relevance of our novel method in the monitoring of critically ill patients.


CONCLUSIONS
This simple and inexpensive kinetic assay can be widely used in any routine laboratory interested in oxidative stress research. It is especially recommended for monitoring critically ill or other patients.
Selmeci
L
L
Department of Cardiovascular Surgery, Faculty of Medicine, Semmelweis University, Városmajor u. 68, H-1122 Budapest, Hungary. laszlo.selmeci@excite.com
Seres
L
L
Soós
P
P
Székely
M
M
Acsády
Gy
G
D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001681:Biological Assay / Q000379:methods; D015415:Biomarkers / Q000097:blood; D001774:Blood Chemical Analysis / Q000379:methods*; D001798:Blood Proteins / Q000032:analysis* / Q000378:metabolism; D002318:Cardiovascular Diseases / Q000097:blood*; D002700:Chloramines / Q000032:analysis; D005260:Female; D006801:Humans; D007202:Indicators and Reagents / Q000032:analysis; D007700:Kinetics; D008297:Male; D008875:Middle Aged; D018384:Oxidative Stress; D016491:Peripheral Vascular Diseases / Q000097:blood*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014105:Tosyl Compounds / Q000032:analysis
D015415:Biomarkers; D001798:Blood Proteins; D002700:Chloramines; D007202:Indicators and Reagents; D014105:Tosyl Compounds; C016300:chloramine-T