[An improved replica plating method and its application to the high-throughput selection of transformants].
OBJECTIVE
To make a simpler and highly efficient replica plating tool to avoid the limitations of the traditional method.


METHODS
A number of bamboo-toothpicks was sheaved to form a bundle with diameter close to the plate diameter. The sheaved bamboo-toothpicks were used for replica-plating.


RESULTS
This replica plating tool was successfully used for the screening of Saccahromyces cerevisiae transformants.


CONCLUSIONS
Compared with the traditional tool, this tool was simpler and highly efficient.
Xu
Minjun
M
State Key Laboratory for Biohazard Control, College of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, China. xuminjun1974@yahoo.com.cn
Tian
Xiaoqun
X
Zhou
Shining
S
D003365:Costs and Cost Analysis; D005818:Genetic Engineering / Q000295:instrumentation*; D016133:Polymerase Chain Reaction; D012441:Saccharomyces cerevisiae / Q000235:genetics* / Q000302:isolation & purification*; D013997:Time Factors; D014170:Transformation, Genetic