title: Cell cycle-independent expression of immediate-early gene 3 results in G1 and G2 arrest in murine cytomegalovirus-infected cells.
abstract: The infectious cycle of human cytomegalovirus (HCMV) is intricately linked to the host's cell cycle. Viral gene expression can be initiated only in G(0)/G(1) phase. Once expressed, the immediate-early gene product IE2 prevents cellular DNA synthesis, arresting infected cells with a G(1) DNA content. This function is required for efficient viral replication in vitro. A prerequisite for addressing its in vivo relevance is the characterization of cell cycle-regulatory activities of CMV species for which animal models have been established. Here, we show that murine CMV (MCMV), like HCMV, has a strong antiproliferative capacity and arrests cells in G(1). Unexpectedly, and in contrast to HCMV, MCMV can also block cells that have passed through S phase by arresting them in G(2). Moreover, MCMV can also replicate in G(2) cells. This is made possible by the cell cycle-independent expression of MCMV immediate-early genes. Transfection experiments show that of several MCMV candidate genes, only immediate-early gene 3 (ie3), the homologue of HCMV IE2, exhibits cell cycle arrest activity. Accordingly, an MCMV ie3 deletion mutant has lost the ability to arrest cells in either G(1) or G(2). Thus, despite interspecies variations in the cell cycle dependence of viral gene expression, the central theme of HCMV IE2-induced cell cycle arrest is conserved in the murine counterpart, raising the possibility of studying its physiological relevance at the level of the whole organism.
authors.affiliation: Department of Pediatrics, Laboratory for Molecular Biology, Charité-Universitätsmedizin Berlin, Ziegelstr. 5-9, 10117 Berlin, Germany.
authors.forename: Lüder
authors.initials: L
authors.lastname: Wiebusch
authors.forename: Anke
authors.initials: A
authors.lastname: Neuwirth
authors.forename: Linus
authors.initials: L
authors.lastname: Grabenhenrich
authors.forename: Sebastian
authors.initials: S
authors.lastname: Voigt
authors.forename: Christian
authors.initials: C
authors.lastname: Hagemeier
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004261:DNA Replication; D016193:G1 Phase / Q000502:physiology*; D016195:G2 Phase / Q000502:physiology*; D015967:Gene Expression Regulation, Viral; D017781:Genes, Immediate-Early / Q000235:genetics*; D006801:Humans; D017874:Immediate-Early Proteins / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018146:Muromegalovirus / Q000235:genetics* / Q000472:pathogenicity / Q000502:physiology*; D041681:NIH 3T3 Cells; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: C069186:IE2 protein, Cytomegalovirus; D017874:Immediate-Early Proteins; D015534:Trans-Activators