Characterization of the glycine betaine biosynthetic genes in the moderately halophilic bacterium Halobacillus dabanensis D-8(T).
The gene cluster involved in the choline-glycine betaine conversion pathway was cloned from chromosomal DNA of the Gram-positive moderate halophile Halobacillus dabanensis D-8(T). Nucleotide sequence analysis revealed four genes, designated gbsT, gbsI, gbsA, and gbsB, which are clustered in a 5.1-kb fragment. After heterologous expression of gbsAB in the Escherichia coli mutant strain PD141, the transformed cells were able to grow in a selective M63 medium containing 0.7 M NaCl and 1 mM choline, in contrast to the mutant strain. Glycine betaine biosynthesis was restored and its accumulation was confirmed by using (13)C nuclear magnetic resonance spectroscopy.
Gu
Zhi Jing
ZJ
Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, and Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, No. 2 Yuanmingyuan West Road, Beijing, 100193, China.
Wang
Lei
L
Le Rudulier
Daniel
D
Zhang
Bo
B
Yang
Su Sheng
SS
D000595:Amino Acid Sequence; D001406:Bacillaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism / Q000502:physiology*; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D001622:Betaine / Q000378:metabolism*; D002794:Choline / Q000378:metabolism; D003470:Culture Media; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D009682:Magnetic Resonance Spectroscopy / Q000379:methods; D008969:Molecular Sequence Data; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism* / Q000494:pharmacology; D014882:Water-Electrolyte Balance
D001426:Bacterial Proteins; D003470:Culture Media; D004269:DNA, Bacterial; D001622:Betaine; D012965:Sodium Chloride; D002794:Choline