title: Immunohistochemical expression of markers Ki-67, neun, synaptophysin, p53 and HER2 in medulloblastoma and its correlation with clinicopathological parameters.
abstract: Medulloblastoma (MB) is the most common malignant brain tumor in childhood. The alterations found include: presence of oncoproteins p53 and HER2, elevated mitotic index, and presence of neuronal differentiation. The aim of this study was to determine the immunohistochemical expression of markers Ki-67, NeuN, synaptophysin, HER2 and p53 in 40 MB samples and their correlation with clinicopathologic parameters and survival. In 29 patients (72.5%), >20% of cells were positive for Ki-67. Males showed greater ki-67 expression (p=0.02) and smaller survival rates (p=0.002). NeuN and synaptophysin were negative in 16 (40%) and 8 (20%) cases, respectively. P53 was positive in 18 (45%) cases, with 11 (61%) weakly positive and 7 (39%) strongly positive. HER2 was positive in 23 (57.5%) of the samples and did not show statistical association with survival (p=0.07).
authors.affiliation: Postgraduate Program in Pathology, Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, RS, Brazil. rosalvameurer@hotmail.com
authors.forename: Rosalva Thereza
authors.initials: RT
authors.lastname: Meurer
authors.forename: Daniele Tondolo
authors.initials: DT
authors.lastname: Martins
authors.forename: Arlete
authors.initials: A
authors.lastname: Hilbig
authors.forename: Marlise de Castro
authors.initials: Mde C
authors.lastname: Ribeiro
authors.forename: Adriana Vial
authors.initials: AV
authors.lastname: Roehe
authors.forename: Ligia Maria
authors.initials: LM
authors.lastname: Barbosa-Coutinho
authors.forename: Marilda da Cruz
authors.initials: Mda C
authors.lastname: Fernandes
mesh_terms: D000293:Adolescent; D034961:Antigens, Nuclear / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000378:metabolism*; D001938:Brazil / Q000453:epidemiology; D002528:Cerebellar Neoplasms / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D002648:Child; D002675:Child, Preschool; D004812:Epidemiologic Methods; D005260:Female; D006801:Humans; D007223:Infant; D019394:Ki-67 Antigen / Q000378:metabolism; D008297:Male; D008527:Medulloblastoma / Q000378:metabolism / Q000401:mortality / Q000473:pathology*; D018365:Neoplasm, Residual; D009419:Nerve Tissue Proteins / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism; D016708:Synaptophysin / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000378:metabolism
chemical_list: D034961:Antigens, Nuclear; D014408:Biomarkers, Tumor; D019394:Ki-67 Antigen; D009419:Nerve Tissue Proteins; D018719:Erb-b2 Receptor Tyrosine Kinases; D016708:Synaptophysin; D016159:Tumor Suppressor Protein p53; C506808:neuronal nuclear antigen NeuN, human