title: Expression of frog virus 3 genes is impaired in mammalian cell lines.
abstract: Frog virus 3 (FV3) is a large DNA virus that is the prototypic member of the family Iridoviridae. To examine levels of FV3 gene expression we generated a polyclonal antibody against the FV3 protein 75L. Following a FV3 infection in fathead minnow (FHM) cells 75L was found in vesicles throughout the cytoplasm as early as 3 hours post-infection. While 75L expressed strongly in FHM cells, our findings revealed no 75L expression in mammalian cells lines despite evidence of a FV3 infection. One explanation for the lack of gene expression in mammalian cell lines may be inefficient codon usage. As a result, 75L was codon optimized and transfection of the codon optimized construct resulted in detectable expression in mammalian cells. Therefore, although FV3 can infect and replicate in mammalian cell lines, the virus may not express its full complement of genes due to inefficient codon usage in mammalian species.
authors.affiliation: Department of Biology, Trent University, Peterborough, ON, Canada. heathereaton@trentu.ca
authors.forename: Heather E
authors.initials: HE
authors.lastname: Eaton
authors.forename: Julie
authors.initials: J
authors.lastname: Metcalf
authors.forename: Craig R
authors.initials: CR
authors.lastname: Brunetti
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002460:Cell Line; D002522:Chlorocebus aethiops; D003062:Codon / Q000235:genetics; D003530:Cyprinidae / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008322:Mammals / Q000235:genetics / Q000378:metabolism* / Q000821:virology*; D008969:Molecular Sequence Data; D017995:Ranavirus / Q000235:genetics* / Q000378:metabolism
chemical_list: D003062:Codon