Inhibitory effect of the compounds isolated from Rhus verniciflua on aldose reductase and advanced glycation endproducts.
The aim of this paper was to evaluate active principles for diabetic complications from Rhus verniciflua. Nine compounds were isolated via bioactivity guided fractionation and isolation and tested for their effects on recombinant human aldose reductase and advanced glycation endproducts. Butein and sulfuretin isolated from ethyl acetate fraction were found to possess strongly both forms of aldose reductase and advanced glycation endproducts inhibition. The inhibitory activity of butein against a recombinant human aldose reductase (IC(50) value: 0.5 microM) was 2.6 times more potent that of epalrestat as a positive control (IC(50) value: 1.3 microM). The inhibitory potency of sulfuretin (IC(50) value: 124.7 microM) on advanced glycation end-products was about 10 times more potent that of aminoguanidine as a positive control (IC(50) value: 1231.0 microM). These compounds all displayed antioxidative activity which was measured by Photochem apparatus. It was concluded, therefore, butein and sulfuretin have antioxidative as well as aldose reductase and advanced glycation endproducts inhibitory effects. As a result, these compounds could be proposed as a leading compound for further study as a new natural products drug that could be used for diabetic complications.
Lee
Eun Ha
EH
Natural Products Research Center, Korea Institute of Science and Technology (KIST) Gangneung Institute, Gangneung, Korea.
Song
Dae-Geun
DG
Lee
Joo Young
JY
Pan
Cheol-Ho
CH
Um
Byung Hun
BH
Jung
Sang Hoon
SH
D000449:Aldehyde Reductase / Q000037:antagonists & inhibitors*; D001572:Benzofurans / Q000737:chemistry / Q000494:pharmacology; D047188:Chalcones / Q000737:chemistry / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000494:pharmacology; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors*; D006801:Humans; D007700:Kinetics; D024301:Plant Bark / Q000737:chemistry; D011994:Recombinant Proteins / Q000378:metabolism; D029045:Rhus / Q000737:chemistry*
D001572:Benzofurans; D047188:Chalcones; D004791:Enzyme Inhibitors; D005419:Flavonoids; D016166:Free Radical Scavengers; D017127:Glycation End Products, Advanced; D011994:Recombinant Proteins; C040918:butein; C484670:AKR1B1 protein, human; D000449:Aldehyde Reductase; C054989:sulfuretin