title: Ingress of blood-borne macrophages across the blood-brain barrier in murine HIV-1 encephalitis.
abstract: Blood-borne macrophage ingress into brain in HIV-1 associated neurocognitive disorders governs the tempo of disease. We used superparamagnetic iron-oxide particles loaded into murine bone marrow-derived macrophages (BMM) injected intravenously into HIV-1 encephalitis mice to quantitatively assess BMM entry into diseased brain regions. Magnetic resonance imaging tests were validated by histological coregistration and enhanced image processing. The demonstration of robust BMM migration into areas of focal encephalitis provide 'proof of concept' for the use of MRI to monitor macrophage ingress into brain.
authors.affiliation: Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198-5880, United States.
authors.forename: Yutong
authors.initials: Y
authors.lastname: Liu
authors.forename: Mariano G
authors.initials: MG
authors.lastname: Uberti
authors.forename: Huanyu
authors.initials: H
authors.lastname: Dou
authors.forename: Rebecca
authors.initials: R
authors.lastname: Banerjee
authors.forename: Cassi B
authors.initials: CB
authors.lastname: Grotepas
authors.forename: David K
authors.initials: DK
authors.lastname: Stone
authors.forename: Barrett E
authors.initials: BE
authors.lastname: Rabinow
authors.forename: Howard E
authors.initials: HE
authors.lastname: Gendelman
authors.forename: Michael D
authors.initials: MD
authors.lastname: Boska
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier / Q000473:pathology / Q000503:physiopathology*; D002465:Cell Movement / Q000502:physiology; D004195:Disease Models, Animal; D004660:Encephalitis / Q000209:etiology* / Q000473:pathology* / Q000821:virology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D015658:HIV Infections / Q000150:complications*; D021621:Imaging, Three-Dimensional; D008264:Macrophages / Q000473:pathology / Q000502:physiology*; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D016513:Mice, SCID; D014746:Vimentin / Q000378:metabolism
chemical_list: D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin