title: [Changes of proteomic spectra of total flavones of Hippophae rhamnoides on myocardial protection].
abstract: OBJECTIVE
  To study the mechanisms of the anti-myocardial ischemia of total flavones of Hippophae rhamnoides (TFH) at the level of proteome.
  
  
  METHODS
  Surface enhanced laser desorption/ionization (SELDI) mass spectrometry with protein chip IMAC3, SAX2 and NP20 was performed to compare the differentially expressed protein in myocardial ischemia in the TFH-treated groups with the 0.9% sodium chloride groups. Protein chips were examined in PBS II - C protein chip reader (ciphergen ciosystem inc) and the protein profiling was analyzed by Proteinchip Software 3. 0. 2.
  
  
  RESULTS
  The revealed six peaks had significant difference between the TFH-treated groups and the control groups, one of which were up-regulated in the TFH-treated groups, and the other were down-regulated. And in these six distinct proteins, there were four proteins on the IMAC3 chips and one protein on the SAX2 chips.
  
  
  CONCLUSIONS
  The TFH could prevent the myocardium from ischemia via regulating expression of different proteins.
authors.affiliation: Department of Cardiovascular Research, Baotou Medical college, Baotou 014010, China. lzr13039570839@sina.com
authors.forename: Zheng-Ran
authors.initials: ZR
authors.lastname: Liu
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.forename: Yu-Mei
authors.initials: YM
authors.lastname: Yang
authors.forename: Hui
authors.initials: H
authors.lastname: Guan
authors.forename: Feng-Ming
authors.initials: FM
authors.lastname: Liu
mesh_terms: D000818:Animals; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D047309:Flavones / Q000737:chemistry / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects*; D031281:Hippophae / Q000737:chemistry*; D008297:Male; D017202:Myocardial Ischemia / Q000517:prevention & control; D040901:Proteomics / Q000379:methods*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D004365:Drugs, Chinese Herbal; D047309:Flavones