Mixed micellar liquid chromatography methods: modelling quantitative retention-activity relationships of angiotensin converting enzyme inhibitors.
The capability of biopartitioning micellar chromatography (BMC), using pure Brij35 solution and mixed micellar system of Brij35-SDS (85:15) as mobile phase, to describe and estimate bioactivities of angiotensin converting enzyme inhibitors at different pH has been studied. Quantitative retention-activity relationships (QRAR) in BMC were investigated for these compounds. The obtained BMC(Brij35-SDS)-QRAR models were compared with the traditional BMC(Brij35)-QRAR, and better statistically models were obtained using Brij35-SDS retention data. The superiority of BMC(Brij35-SDS)-QRAR is due to the fact that the mixed micellar mobile phase can simulate the resting membrane potential and the conformation of the long hydrophilic polyoxyethylene chains remains unchanged.
Wu
Li-Ping
LP
West China School of Pharmacy, Sichuan University, Chengdu, 610041, People's Republic of China.
Cui
Yan
Y
Xiong
Mei-Jin
MJ
Wang
Shu-Rong
SR
Chen
Cong
C
Ye
Li-Ming
LM
D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000493:pharmacokinetics* / Q000494:pharmacology; D002853:Chromatography, Liquid / Q000379:methods*; D008823:Micelles; D008954:Models, Biological; D011092:Polyethylene Glycols / Q000737:chemistry; D013329:Structure-Activity Relationship
D000806:Angiotensin-Converting Enzyme Inhibitors; D008823:Micelles; D011092:Polyethylene Glycols; C515901:Brij 35