Combinatorial surrobody libraries.
A unique type of combinatorial protein libraries has been constructed. These libraries are based on the pre-B cell receptor (pre-BCR). The pre-BCR is a protein that is produced during normal development of the antibody repertoire. Unlike that of canonical antibodies, the pre-BCR subunit is a trimer that is composed of an antibody heavy chain paired with two surrogate light chain (SLC) components. Combinatorial libraries based on these pre-BCR proteins in which diverse heavy chains are paired with a fixed SLC were expressed in mammalian, Escherichia coli, and phagemid systems. These libraries contain members that have nanomolar affinity for antigen. We term this type of antigen-binding protein a "surrobody" to distinguish it from the canonical antibody molecule.
Xu
Li
L
Sea Lane Biotechnologies, 1455 Adams Drive, Suite 2060, Menlo Park, CA 94025, USA.
Yee
Helena
H
Chan
Christina
C
Kashyap
Arun K
AK
Horowitz
Lawrence
L
Horowitz
Michael
M
Bhatt
Ramesh R
RR
Lerner
Richard A
RA
D000906:Antibodies / Q000235:genetics*; D008958:Models, Molecular; D019151:Peptide Library; D054420:Pre-B Cell Receptors / Q000235:genetics*
D000906:Antibodies; D019151:Peptide Library; D054420:Pre-B Cell Receptors