title: Combinatorial surrobody libraries.
abstract: A unique type of combinatorial protein libraries has been constructed. These libraries are based on the pre-B cell receptor (pre-BCR). The pre-BCR is a protein that is produced during normal development of the antibody repertoire. Unlike that of canonical antibodies, the pre-BCR subunit is a trimer that is composed of an antibody heavy chain paired with two surrogate light chain (SLC) components. Combinatorial libraries based on these pre-BCR proteins in which diverse heavy chains are paired with a fixed SLC were expressed in mammalian, Escherichia coli, and phagemid systems. These libraries contain members that have nanomolar affinity for antigen. We term this type of antigen-binding protein a "surrobody" to distinguish it from the canonical antibody molecule.
authors.affiliation: Sea Lane Biotechnologies, 1455 Adams Drive, Suite 2060, Menlo Park, CA 94025, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.forename: Helena
authors.initials: H
authors.lastname: Yee
authors.forename: Christina
authors.initials: C
authors.lastname: Chan
authors.forename: Arun K
authors.initials: AK
authors.lastname: Kashyap
authors.forename: Lawrence
authors.initials: L
authors.lastname: Horowitz
authors.forename: Michael
authors.initials: M
authors.lastname: Horowitz
authors.forename: Ramesh R
authors.initials: RR
authors.lastname: Bhatt
authors.forename: Richard A
authors.initials: RA
authors.lastname: Lerner
mesh_terms: D000906:Antibodies / Q000235:genetics*; D008958:Models, Molecular; D019151:Peptide Library; D054420:Pre-B Cell Receptors / Q000235:genetics*
chemical_list: D000906:Antibodies; D019151:Peptide Library; D054420:Pre-B Cell Receptors