Conformational dynamics of an intact virus: order parameters for the coat protein of Pf1 bacteriophage.
This study has examined the atomic-level dynamics of the protein in the capsid of filamentous phage Pf1. This capsid consists of approximately 7,300 small subunits of only 46 aa in a helical array around a highly extended, circular single-stranded DNA molecule of 7,349 nt. Measurements were made of site-specific, solid-state NMR order parameters, S, the values which are dimensionless quantities between 0 (mobile) and 1 (static) that characterize the amplitudes of molecular bond angular motions that are faster than microseconds. It was found that the protein subunit backbone is very static, and of particular interest, it appears to be static at residues glycine 15 and glutamine 16 where it had been previously thought to be mobile. In contrast to the backbone, several side chains display large-amplitude angular motions. Side chains on the virion exterior that interact with solvent are highly mobile, but surprisingly, the side chains of residues arginine 44 and lysine 45 near the DNA deep in the interior of the virion are also highly mobile. The large-amplitude dynamic motion of these positively charged side chains in their interactions with the DNA were not previously expected. The results reveal a highly dynamic aspect of a DNA-protein interface within a virus.
Lorieau
Justin L
JL
Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027.
Day
Loren A
LA
McDermott
Ann E
AE
D001120:Arginine / Q000737:chemistry; D025561:Bacteriophage Pf1 / Q000737:chemistry*; D001435:Bacteriophages / Q000378:metabolism*; D036022:Capsid Proteins / Q000737:chemistry; D004247:DNA / Q000737:chemistry; D004277:DNA, Single-Stranded / Q000737:chemistry; D004279:DNA, Viral / Q000737:chemistry; D005973:Glutamine / Q000737:chemistry; D005998:Glycine / Q000737:chemistry; D008239:Lysine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008968:Molecular Conformation; D012997:Solvents / Q000737:chemistry
D036022:Capsid Proteins; D004277:DNA, Single-Stranded; D004279:DNA, Viral; D012997:Solvents; D005973:Glutamine; D004247:DNA; D001120:Arginine; D008239:Lysine; D005998:Glycine