title: Analysis of selected stilbenes in Polygonum cuspidatum by HPLC coupled with CoulArray detection.
abstract: The roots of three varieties of Polygonum cuspidatum were analyzed for resveratrol and its analogs. The powder of the dried roots was extracted with aqueous ethanol (60% v/v) and the extracts obtained were analyzed using RP HPLC with coulometric detection. A simple HPLC method with a multichannel CoulArray detector was developed for the determination of four stilbenes: resveratrol, its glucoside piceid, piceatannol, and its glucoside astringin. Analyses were carried out on a LiChrospher C18 (125 x 4.6 mm id, particle size 5 microm) column with a mobile phase of ammonium acetate (pH 3) and ACN in gradient mode. Four compounds were monitored by a CoulArray electrochemical detector. Potentials of eight electrochemical cells in series were set in the range of 200-900 mV. Optimization of the mobile phase pH was performed. Calibration curves showed good linearity with correlation coefficients (r(2))--more than 0.9975.
authors.affiliation: Research Institute of Organic Syntheses, Pardubice 20, Czech Republic. blanka.benova@email.cz
authors.forename: Blanka
authors.initials: B
authors.lastname: Benová
authors.forename: Martin
authors.initials: M
authors.lastname: Adam
authors.forename: Katerina
authors.initials: K
authors.lastname: Onderková
authors.forename: Josef
authors.initials: J
authors.lastname: Královský
authors.forename: Milan
authors.initials: M
authors.lastname: Krajícek
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D004563:Electrochemistry / Q000295:instrumentation; D045468:Fallopia japonica / Q000737:chemistry*; D005960:Glucosides / Q000032:analysis; D006863:Hydrogen-Ion Concentration; D018517:Plant Roots / Q000737:chemistry; D000077185:Resveratrol; D013267:Stilbenes / Q000032:analysis*
chemical_list: D005960:Glucosides; D013267:Stilbenes; C121481:astringin; C041525:3,3',4,5'-tetrahydroxystilbene; D000077185:Resveratrol; C058229:polydatin