[Isolation and identification of Suavissimoside R1 from roots of Rubus parvifollus used for protecting dopaminergic neurons against MPP+ toxicity].
OBJECTIVE
Suavissimoside R1 was isolated and identified as an active ingredient from Roots of Rubus parvifollus L, which exhibited protective effect on dopaminergic neurons against MPP+ toxicity.


METHODS
The protective effects of crude extracts were investigated after mice were treated with 1-methyl4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). According to the protective effects of crude extracts, suavissimoside R1, one kind of triterpenoid saponin, was separated. It was investigated that whether Suavissimoside R1 can protect DA neurons from toxicity induced by MPP+ in rat mesencephalic cultures.


RESULTS
Suavissimoside R1 was isolated from Roots of Rubus parvifollus L. Moreover, Suavissimoside R1, in dose of 100 micromol/L, alleviated the death of DA neurons induced by MPP+ obviously.


CONCLUSIONS
These results suggest that suavissimoside R1 possesses potent neuroprotective activity and can be developed to be a potential anti-Parkinson's disease drug worthy for further study.
Yu
Zhan-Yang
ZY
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China. yuzhy@mail.sysu.edu.cn
Ruan
Hao-Lan
HL
Zhu
Xiao-Nan
XN
Wang
Xue-Lan
XL
Chen
Ru-Zhu
RZ
Lin
Yong-Cheng
YC
D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000818:Animals; D003342:Corpus Striatum / Q000378:metabolism; D004298:Dopamine / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D018696:Neuroprotective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D010300:Parkinson Disease / Q000378:metabolism / Q000517:prevention & control; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley; D027824:Rosaceae / Q000737:chemistry*; D012503:Saponins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*
D018696:Neuroprotective Agents; D010936:Plant Extracts; D012503:Saponins; C544119:suavissimoside R1; D015655:1-Methyl-4-phenylpyridinium; D004298:Dopamine