Proinflammatory cytokines, fibrinolytic system enzymes, and biochemical indices in children with infectious para-pneumonic effusions.
BACKGROUND
In children, pleural empyema is a recognized complication of severe pneumonia and is characterized by loculated effusions with fibrin septations. The aim of this study was to evaluate the relationship between proinflammatory cytokines [tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-6], intrapleural fibrinolytic system enzymes [tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor type 1 (PAI-1)], and common biochemical indices during pleural infection.


METHODS
Children with pneumonia complicated by para-pneumonic effusions were enrolled into our study and underwent real-time chest sonography. The patients were divided into 3 groups by ultrasound using a recognized staging system of pleural effusions. Staging of progressive pleural infection was used to correlate with the characteristics of pleural effusions. The correlation of various pleural variables with the formation of complicated para-pneumonic effusions (CPE) was performed and pleural variables for predicting subsequent intervention procedures were also analyzed.


RESULTS
A total of 57 patients were enrolled in the present study. Univariate analysis revealed that the amounts of biochemical indices (pH, glucose, lactate dehydrogenase), proinflammatory cytokines (TNF-alpha, IL-1beta, IL-6), and fibrinolytic system enzymes (tPA, PAI-1) were significantly different with the progressive stages of para-pneumonic effusions (Ptrend < 0.05). For all proinflammatory cytokines, a positive correlation was found with lactate dehydrogenase and PAI-1, whereas a negative correlation was found with pH, glucose, and tPA. Moreover, these cytokines were also significantly correlated with PAI-1 in both non-CPE and CPE. The pleural fluid findings of IL-1beta (> or =50 pg/mL), PAI-1 (> or =1252 ng/mL), and pH (< or =7.30) were the most significant predictive factors for subsequent intervention procedures (P < 0.001).


CONCLUSIONS
The increased release of proinflammatory cytokines in pleural fluid caused by bacteria may result in an imbalance of the fibrinolytic system, which can subsequently lead to fibrin deposition and intervention procedures.
Chiu
Chih-Yung
CY
Graduate Institute of Clinical Medical Sciences, Chang Gung University, Taoyuan, Taiwan.
Wong
Kin-Sun
KS
Huang
Jing-Long
JL
Tasi
Ming-Han
MH
Lin
Tzou-Yien
TY
Hsieh
Sen-Yung
SY
D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D016207:Cytokines / Q000378:metabolism*; D005260:Female; D005337:Fibrin / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007223:Infant; D007249:Inflammation / Q000276:immunology*; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D008297:Male; D017395:Plasminogen Activator Inhibitor 1 / Q000378:metabolism*; D010996:Pleural Effusion / Q000000981:diagnostic imaging / Q000276:immunology / Q000378:metabolism; D018410:Pneumonia, Bacterial / Q000000981:diagnostic imaging / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D011018:Pneumonia, Pneumococcal / Q000000981:diagnostic imaging / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D010959:Tissue Plasminogen Activator / Q000378:metabolism*; D014463:Ultrasonography
D016207:Cytokines; D017395:Plasminogen Activator Inhibitor 1; D005337:Fibrin; D007770:L-Lactate Dehydrogenase; D010959:Tissue Plasminogen Activator; D005947:Glucose