In vitro assessment of adhesion molecules expression by human endothelial cells cocultured with c-erbB2-positive and c-erbB2-negative breast carcinoma cell lines.
OBJECTIVE
In vitro assessment of ICAM-1, VCAM-1 and P-selectin expression on endothelial cells cocultured with c-erbB2-positive (SK-BR-3) and c-erbB2-negative (MCF-7) breast carcinoma cell lines.


METHODS
The expression of adhesion molecules was determined by immunofluorescence, and measured by a laser scanning cytometer.


RESULTS
Endothelial cells cocultured with c-erbB2-negative breast carcinoma cell line--as compared to endothelial cells cultured without carcinoma cells--demonstrated an increase in the expression of VCAM-1 and P-selectin, a decrease in the expression of ICAM-1, and an increase of the maximal pixel intensity of ICAM-1, VCAM-1 and P-selectin orange fluorescence. A higher increase of the expression and maximal pixel intensity of ICAM-1, VCAM-1 and P-selectin was observed in endothelial cells cocultured with c-erbB2-positive breast carcinoma cells as compared to endothelial cells cocultured with c-erbB2-negative cell line.


CONCLUSIONS
An increase in the expression of adhesion molecules on endothelial cells cocultured with c-erbB2-positive breast carcinoma cells may be responsible for the increased adhesion of tumor cells to endothelium and the increased invasiveness of c-erbB2-positive breast carcinomas.
Markowska
Agnieszka
A
Department of Pathology, Pomeranian Medical University, Szczecin.
Urasińska
Elzbieta
E
Domagała
Wenancjusz
W
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D002448:Cell Adhesion / Q000502:physiology; D015815:Cell Adhesion Molecules / Q000378:metabolism*; D045744:Cell Line, Tumor; D018920:Coculture Techniques; D004730:Endothelium, Vascular / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000473:pathology; D019007:P-Selectin / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000378:metabolism*; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism
D014408:Biomarkers, Tumor; D015815:Cell Adhesion Molecules; D018799:Intercellular Adhesion Molecule-1; D019007:P-Selectin; D018719:Erb-b2 Receptor Tyrosine Kinases; D019010:Vascular Cell Adhesion Molecule-1