Experimental optimization of fed-batch culture for poly-beta-hydroxybutyric acid production.
The optimal feed rate profiles of glucose and ammonium hydroxide were calculated using a proposed model, and implemented for the production of poly-beta-hydroxybutyric acid (PHB) by Alcaligenes eutrophus. By implementing these optimal feed rates with a high glucose feed concentration of 700 g/L and an ammonium hydroxide concentration of 7%(w/w), it was possible to achieve a high final cell concentration of 141 g/L and a high PHB concentration of 105 g/L in 40 h of fed-batch operation. The PHB productivity was as high as 2.63 g/(L hr). (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 697-705, 1997.
Lee
J H
JH
Department of Chemical & Biochemical Engineering, University of California, Irvine, California 92697-2575; telephone: (714) 824-8277; fax: (714) 824-2541.
Hong
J
J
Lim
H C
HC