Emdogain stimulates matrix degradation by osteoblasts.
Emdogain has been used clinically for periodontal regeneration, although the underlying molecular mechanisms are not clear at present. In this study, we hypothesized that Emdogain stimulated degradation of type I collagen via osteoblasts. We showed that Emdogain enhanced cell-mediated degradation of type I collagen in an MMP-dependent manner. Although MG-63 cells spontaneously produced a zymogen form of MMP-1, treatment with Emdogain significantly induced the generation of the active form of this enzyme. We demonstrated that MMP-3 was produced from MG63 cells in response to Emdogain in a MEK1/2-dependent manner. Concomitantly, blocking of MEK1/2 activation by U0126 significantly inhibited the generation of the active form of MMP-1 without affecting the total production of this collagenase. These results suggest that Emdogain facilitates tissue regeneration through the activation of the collagenase, MMP-1, that degrades matrix proteins in bone tissue microenvironments.
Goda
S
S
Departments of Biochemistry, Osaka Dental University, Osaka, Japan. goda@cc.osaka-dent.ac.jp
Inoue
H
H
Kaneshita
Y
Y
Nagano
Y
Y
Ikeo
T
T
Ikeo
Y T
YT
Iida
J
J
Domae
N
N
D001861:Bone Regeneration / Q000502:physiology; D002460:Cell Line; D024042:Collagen Type I / Q000378:metabolism*; D003746:Dental Enamel Proteins / Q000494:pharmacology*; D005109:Extracellular Matrix / Q000201:enzymology / Q000378:metabolism; D006801:Humans; D048369:MAP Kinase Kinase 1 / Q000378:metabolism; D048730:MAP Kinase Kinase Kinase 2 / Q000378:metabolism; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D020784:Matrix Metalloproteinase 8 / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism*; D010510:Periodontal Diseases / Q000188:drug therapy* / Q000378:metabolism
D024042:Collagen Type I; D003746:Dental Enamel Proteins; C109069:enamel matrix proteins; D048730:MAP Kinase Kinase Kinase 2; D048369:MAP Kinase Kinase 1; D020784:Matrix Metalloproteinase 8; D020781:Matrix Metalloproteinase 1