[Effects of 5 classical recipes on anti-oxidative stress in rat liver with cirrhosis].
OBJECTIVE
To compare the anti-oxidative stress effects of 5 classical traditional Chinese herbal recipes in rats with dimethylnitrosamine (DMN)-induced cirrhosis.


METHODS
DMN was injected intraperitoneally three consecutive days a week to male Wistar rats for 4 weeks to develop cirrhosis model, and the model cirrhotic rats were randomly grouped and administered with 5 traditional Chinese classic recipes, i. e. Yiguanjian; Huangqi Decoction; Yinchenhao Decoction; Xiayuxue Decoction and Xiaochaihu Decoction, by gastrogavage, respectively, for 2 weeks. Changes in activity of total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), glutathione-S-transferase (GST) and malondialdehyde (MDA) content in liver tissue, and serum parameters of liver function were determined.


RESULTS
GST activity and MDA content increased significantly in model rats, peaked at week 2 (liver fibrosis formation period) and week 4 (cirrhosis formation period), respectively, while activity of T-SOD and GSH-Px decreased markedly. As compared with the model control group at week 6, the degree of cirrhosis was evidently milder with lower level of MDA and GST and higher T-SOD activity in rats treated by Huangqi Decoction and Yinchenhao Decoction.


CONCLUSIONS
Anti-oxidative stress action should be one important mechanism for Yinchenhao Decoction and Huangqi Decoction in reversing liver cirrhosis of rats. The action of Yinchenhao Decoction focuses on eliminating hepatic lipid peroxide and that of Huangqi Decoction emphasizes for enhancing anti-oxidation ability of the organism.
Wang
Lei
L
Institute of Liver Disease, Shanghai University of TCM, Shanghai.
Liu
Ping
P
Wang
Chun-Shu
CS
D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy; D004195:Disease Models, Animal; D004339:Drug Compounding; D004365:Drugs, Chinese Herbal / Q000627:therapeutic use*; D005979:Glutathione Peroxidase / Q000378:metabolism; D005982:Glutathione Transferase / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008516:Medicine, Chinese Traditional; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism
D004365:Drugs, Chinese Herbal; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005982:Glutathione Transferase