title: Impact of boostering for the strength of asthma parameters and dendritic cell numbers in a C57BL/6 model of allergic airway inflammation.
abstract: BACKGROUND
  Murine models assist in elucidating the pathogenesis of allergic asthma and evaluation of new therapeutic strategies. We aimed to assess the requirement of boostering needed in the BL/6 murine asthma model and its influence on DC populations in lungs and bronchial lymph nodes.
  
  
  RESULTS
  Two injections of OVA+alum - one sensitization and one booster - followed by two aerosol challenges were sufficient to induce a distinct asthma-like inflammation in BL/6 mice, including significant increased immunoglobulin (IgE) level, influx of eosinophils in the airway lumen, and evident histopathology. Using this protocol, CD11chighMHC-II+ DC counts in lungs and lymph nodes doubled with no changes of CD8+ DC in the lungs but increase in lung-draining lymph nodes.
  
  
  CONCLUSIONS
  Given the site-specific changes of dendritic cell (DC) subpopulations during allergic asthma we propose a distinct regulation of antigen transport and antigen presentation in the murine asthma model.
authors.affiliation: Functional and Applied Anatomy, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. hartwig.christina@mh-hannover.de
authors.forename: Christina
authors.initials: C
authors.lastname: Hartwig
authors.forename: Hanne
authors.initials: H
authors.lastname: Constabel
authors.forename: Detlef
authors.initials: D
authors.lastname: Neumann
authors.forename: Heinz
authors.initials: H
authors.lastname: Gerd Hoymann
authors.forename: Thomas
authors.initials: T
authors.lastname: Tschernig
authors.forename: Georg M N
authors.initials: GM
authors.lastname: Behrens
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D001249:Asthma / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D001980:Bronchi / Q000276:immunology* / Q000473:pathology / Q000503:physiopathology; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D002452:Cell Count; D016207:Cytokines / Q000097:blood; D003713:Dendritic Cells / Q000276:immunology* / Q000473:pathology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology / Q000473:pathology; D007073:Immunoglobulin E / Q000097:blood; D008198:Lymph Nodes / Q000276:immunology / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D012129:Respiratory Function Tests
chemical_list: D000485:Allergens; D016207:Cytokines; D007073:Immunoglobulin E; D010047:Ovalbumin