Direct visualization of fluorescent signals in protein gels using a backlit blue light plate.
The visualization of fluorescently labeled protein gels is required for the analysis of electrophoretic results or manually picking protein bands or spots from gels. To accomplish this task, an UV light table is generally utilized, but may be hazardous to operators. The blue light transilluminator is another apparatus that may be used for this purpose, but is sometimes insufficient for revealing weak fluorescent signals. In this study, we invented a new setup utilizing a backlit blue light plate for illuminating fluorescently stained protein gels. This method employs Snell's law and allows for the direct visualization of fluorescent signals in protein gels without the use of a filter or filter glasses. This safe, convenient, economic, and effective setup was found to be an ideal alternative for illuminating fluorescent protein gels in proteomic experiments.
Wu
Szu-Yu
SY
Institute of Applied Science and Engineering, Fu-Jen Catholic University, Taipei, Taiwan.
Chin
Li-Te
LT
Chen
Li-Ming
LM
Chen
Han-Min
HM
D004586:Electrophoresis / Q000379:methods*; D005453:Fluorescence; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results
D011506:Proteins