title: Mass production of Drosophila embryos and chromatographic purification of native protein complexes.
abstract: The purification of native protein complexes requires the availability of sufficient amounts of starting material. Drosophila melanogaster embryos have proven to be a rich source for nuclear protein complexes. Here we describe establishment and maintenance of a fly facility for the production of large amounts of embryos, protocols for the production of nuclear extracts, and a scheme for the chromatographic purification of a nuclear multisubunit protein complex.
authors.affiliation: Institut für Molekularbiologie und Tumorforschung, Philipps-Universität Marburg, Marburg, Germany.
authors.forename: Natascha
authors.initials: N
authors.lastname: Kunert
authors.forename: Alexander
authors.initials: A
authors.lastname: Brehm
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D002845:Chromatography / Q000379:methods*; D015509:Developmental Biology / Q000295:instrumentation / Q000379:methods*; D029721:Drosophila Proteins / Q000032:analysis / Q000302:isolation & purification*; D004331:Drosophila melanogaster / Q000196:embryology*; D004625:Embryo, Nonmammalian / Q000737:chemistry*; D008967:Molecular Biology / Q000295:instrumentation / Q000379:methods*
chemical_list: D029721:Drosophila Proteins