Biochemical indication of microbial mass changes using ATP and DNA measurement in biological treatment of thiocyanate.
This study was designed to monitor changes in the levels of adenosine 5'-triphosphate (ATP) and deoxyribonucleic acid (DNA) per unit of microbial mass during the autotrophic biodegradation of thiocyanate (SCN(-)). An artificial medium containing trace minerals and 500 mg SCN(-)/L was used as a substrate for bacterial growth. An SCN(-)-degrading bioreactor with a working volume of 6 L, equipped with temperature, pH, and dissolved oxygen controls, was operated in batch mode. During the exponential phase of SCN(-) biodegradation, the ratios of ATP and DNA to microbial dry weight varied from 0.6 to 1.1 microg ATP/mg of volatile suspended solid (VSS), and from 3.5 to 8.8 microg DNA/mg of VSS, respectively. The ATP and DNA concentrations correlated linearly with microbial mass (r (2) > 0.9) within the exponential phase. The linear regression equations were as follows: (1) microbial mass concentration (mg/L) = 0.663 x ATP concentration (microg/L) + 11.1 and (2) microbial concentration (mg/L) = 0.081 x DNA concentration (microg/L) + 10.9. The applicable ranges were 6.8 to 47.4 microg/L for ATP concentration and 41.5 to 395 microg/L for DNA concentration, respectively.
Lim
Juntaek
J
School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyungbuk, South Korea.
Lee
Seungyong
S
Kim
Sang-Don
SD
Hwang
Seokhwan
S
D000255:Adenosine Triphosphate / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism; D018533:Biomass; D019149:Bioreactors / Q000382:microbiology; D004269:DNA, Bacterial / Q000378:metabolism*; D012722:Sewage / Q000382:microbiology; D013861:Thiocyanates / Q000378:metabolism*
D004269:DNA, Bacterial; D012722:Sewage; D013861:Thiocyanates; D000255:Adenosine Triphosphate; C031760:thiocyanate