title: Inhibition of lymphocyte CD3 expression by Chlamydophila pneumoniae infection.
abstract: Since lymphocytes are a major immune cell besides macrophages in the development of atherosclerosis, interaction between lymphocytes and Chlamydophila pneumoniae may contribute to the pathogenesis of chronic inflammatory diseases associated with C. pneumoniae. In this regard, we examined a possible alteration of CD3 expression of human lymphocyte Molt-4 cells by C. pneumoniae infection. The expression levels of CD3 molecules of lymphocyte Molt-4 cells were significantly decreased by C. pneumoniae infection. In contrast, heat-killed C. pneumoniae as well as mock (cell lysates) did not cause any alteration of CD3 expression of the cells. Treatment of the infected cells with NS-398 (cyclo-oxyganase-2 inhibitor) or AH-23848 (EP(4) prostanoid receptor antagonist) abolished the inhibition of CD3 expression. The enhanced prostaglandin E(2) (PGE(2)) productions in the culture supernatants of infected cells were confirmed by competitive enzyme-immunosorbent assay (ELISA). C. pneumoniae infection of enriched lymphocytes from human peripheral blood mononuclear cells also induced a decrease of CD3 expression. Thus, C. pneumoniae infection of lymphocytes induces a decrease of CD3 expression mediated by possibly PGE(2) production.
authors.affiliation: Laboratory of Molecular Microbiology, Department of Bioinformatics, Graduate School of Medicine, Osaka University, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan. hiroyuki@med.hokudai.ac.jp
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Yamaguchi
authors.forename: Junji
authors.initials: J
authors.lastname: Matsuo
authors.forename: Shigehiro
authors.initials: S
authors.lastname: Sugimoto
authors.forename: Maki
authors.initials: M
authors.lastname: Utsumi
authors.forename: Yoshimasa
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D017252:CD3 Complex / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D002478:Cells, Cultured; D023521:Chlamydophila Infections / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D016993:Chlamydophila pneumoniae / Q000502:physiology*; D015232:Dinoprostone / Q000235:genetics / Q000378:metabolism; D015536:Down-Regulation; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D006801:Humans; D008214:Lymphocytes / Q000276:immunology* / Q000382:microbiology
chemical_list: D017252:CD3 Complex; D015232:Dinoprostone