Results and reliability of protein quantification for two-dimensional gel electrophoresis strongly depend on the type of protein sample and the method employed.
We investigated the effects of tissue samples taken from rat brain on the reliability of three protein quantification kits: the Bradford assay, the 2-D Quant Kit, and the EZQ Protein Quantitation Kit. All three assays measured significantly smaller amounts of protein after extraction than the reference values before extraction. Only small effects were seen in homogenates, but very pronounced differences in membrane-enriched and highly lipophilic subcellular fractions. Researchers should evaluate which method of protein quantification is best qualified for their specific experimental design.
Weist
Stephanie
S
A+M Proteome Science, Berlin, Germany.
Eravci
Murat
M
Broedel
Oliver
O
Fuxius
Sandra
S
Eravci
Selda
S
Baumgartner
Andreas
A
D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D011506:Proteins / Q000032:analysis* / Q000145:classification; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D015203:Reproducibility of Results
D011506:Proteins