Ingress of blood-borne macrophages across the blood-brain barrier in murine HIV-1 encephalitis.
Blood-borne macrophage ingress into brain in HIV-1 associated neurocognitive disorders governs the tempo of disease. We used superparamagnetic iron-oxide particles loaded into murine bone marrow-derived macrophages (BMM) injected intravenously into HIV-1 encephalitis mice to quantitatively assess BMM entry into diseased brain regions. Magnetic resonance imaging tests were validated by histological coregistration and enhanced image processing. The demonstration of robust BMM migration into areas of focal encephalitis provide 'proof of concept' for the use of MRI to monitor macrophage ingress into brain.
Liu
Yutong
Y
Center for Neurovirology and Neurodegenerative Disorders, University of Nebraska Medical Center, Omaha, NE 68198-5880, United States.
Uberti
Mariano G
MG
Dou
Huanyu
H
Banerjee
Rebecca
R
Grotepas
Cassi B
CB
Stone
David K
DK
Rabinow
Barrett E
BE
Gendelman
Howard E
HE
Boska
Michael D
MD
D000818:Animals; D001812:Blood-Brain Barrier / Q000473:pathology / Q000503:physiopathology*; D002465:Cell Movement / Q000502:physiology; D004195:Disease Models, Animal; D004660:Encephalitis / Q000209:etiology* / Q000473:pathology* / Q000821:virology; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D015658:HIV Infections / Q000150:complications*; D021621:Imaging, Three-Dimensional; D008264:Macrophages / Q000473:pathology / Q000502:physiology*; D008279:Magnetic Resonance Imaging; D008297:Male; D051379:Mice; D016513:Mice, SCID; D014746:Vimentin / Q000378:metabolism
D005904:Glial Fibrillary Acidic Protein; D014746:Vimentin