title: Low- versus high-baseline epinephrine output shapes opposite innate cytokine profiles: presence of Lewis- and Fischer-like neurohormonal immune phenotypes in humans?
abstract: Immunogenetic mechanisms operating within the immune system are known to influence cytokine profiles and disease susceptibility. Yet the role of the individual's neurohormonal background in these processes remains undefined. Hormonal imbalances are documented in immune-related diseases, but it is unclear whether this represents a secondary phenomenon or a primary "defect" related to specific neurohormonal immune phenotype(s). We report that in a large subpopulation of healthy humans the baseline epinephrine output (but not cortisol and sex steroid hormones) correlated inversely with proinflammatory and positively with anti-inflammatory cytokine production. Thus, low vs high epinephrine excretors had a 2- to 5-fold higher TNF-alpha and IL-12 production but 2-fold lower IL-10 production induced by LPS ex vivo. In alternative settings, we found low baseline levels and profoundly blunted stress-induced epinephrine responses but high TNF-alpha levels in Lewis vs Fischer inbred rats. Additionally, isoproterenol, a beta adrenoreceptor agonist suppressed LPS-induced TNF-alpha production, with more pronounced effect in Lewis than in Fischer rats. In human monocytes, epinephrine and the beta(2) adrenoreceptor agonist fenoterol potently inhibited LPS-induced TNF-alpha and IL-12, but stimulated IL-10 production. The order of potency for hormones able to inhibit IL-12 production ex vivo was: epinephrine > norepinephrine > or = 1,25-(OH)(2) vitamin D(3) > hydrocortisone. This indicates that baseline epinephrine conditions cytokine responsiveness and through this mechanism intrinsic hypo- or hyperactive adrenal medullas in some individuals may shape opposite cytokine profiles. Since Lewis and Fischer rats have opposite susceptibility to experimental immunological diseases, this suggests that the parallel human phenotypes could be linked to differing responsiveness and susceptibility to infections and immune/inflammatory-related conditions.
authors.affiliation: Arthritis and Rheumatism Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA. ilia.elenkov@tiscali.it
authors.forename: Ilia J
authors.initials: IJ
authors.lastname: Elenkov
authors.forename: Richard
authors.initials: R
authors.lastname: Kvetnansky
authors.forename: Akira
authors.initials: A
authors.lastname: Hashiramoto
authors.forename: Vladimir K
authors.initials: VK
authors.lastname: Bakalov
authors.forename: Amrey A
authors.initials: AA
authors.lastname: Link
authors.forename: Keith
authors.initials: K
authors.lastname: Zachman
authors.forename: Marianna
authors.initials: M
authors.lastname: Crane
authors.forename: Daniela
authors.initials: D
authors.lastname: Jezova
authors.forename: Jozef
authors.initials: J
authors.lastname: Rovensky
authors.forename: Mariana A
authors.initials: MA
authors.lastname: Dimitrov
authors.forename: Philip W
authors.initials: PW
authors.lastname: Gold
authors.forename: Sergio
authors.initials: S
authors.lastname: Bonini
authors.forename: Thomas
authors.initials: T
authors.lastname: Fleisher
authors.forename: George P
authors.initials: GP
authors.lastname: Chrousos
authors.forename: Ronald L
authors.initials: RL
authors.lastname: Wilder
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D016207:Cytokines / Q000096:biosynthesis* / Q000276:immunology*; D004837:Epinephrine / Q000494:pharmacology*; D006801:Humans; D007113:Immunity, Innate / Q000187:drug effects* / Q000276:immunology*; D008297:Male; D010641:Phenotype; D051381:Rats; D011916:Rats, Inbred F344; D011917:Rats, Inbred Lew; D011943:Receptors, Adrenergic, beta / Q000378:metabolism; D013564:Sympathetic Nervous System / Q000187:drug effects / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D011943:Receptors, Adrenergic, beta; D004837:Epinephrine