title: Gene delivery to cells on a miniaturized multiwell plate for high-throughput gene function analysis.
abstract: The transfectional microarray is a promising tool to conduct high-throughput analysis of gene function. In this study, a miniaturized multiwell plate was prepared by applying a silicone rubber sheet with holes on a gold electrode. The combination of electroporation and a miniaturized multiwell plate successfully achieved spatially controlled gene delivery with absence of cross-contamination between genes. Furthermore, we found that gene delivery efficiency was not dependent on conditions such as plasmid loading, electric field strength, and pulse duration.
authors.affiliation: Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan.
authors.forename: Ellyana
authors.initials: E
authors.lastname: Njatawidjaja
authors.forename: Hiroo
authors.initials: H
authors.lastname: Iwata
mesh_terms: D002460:Cell Line; D018274:Electroporation / Q000295:instrumentation; D015870:Gene Expression; D018014:Gene Transfer Techniques / Q000295:instrumentation*; D006801:Humans; D008904:Miniaturization / Q000295:instrumentation*; D019076:Transgenes / Q000235:genetics*