Gene expression profiles of adipose tissue of obese rats after central administration of neuropeptide Y-Y5 receptor antisense oligodeoxynucleotides by cDNA microarrays.
To investigate the gene expression profiles of adipose tissue of obese rats after central administration of neuropeptide Y-Y5 receptor antisense oligodeoxynucleotides (ODNs), Y5 receptor antisense, mismatched ODNs or vehicle was intracerebroventricularly injected and cDNA microarrays were undertaken. Central administration of NPY-Y5 receptor antisense ODNs decreased food intake, body weight and serum insulin compared with both vehicle and mismatched ODNs. The average area of adipocytes both at retroperitoneal and epididymal adipose tissue were fall in antisense group while only the weight of the retroperitoneal fat pats was reduced in antisense group. cDNA microarrays containing 18,000 genes/Ests were used to investigate gene expression of adipose tissue. Autoradiographic analysis showed that 404, 81, and 34 genes were differently expressed over twofold, threefold, and fivefold, respectively. The analysis of gene expression profiles indicated that 332 genes were up-regulated and 187 genes were down-regulated in response to Y5 receptor antisense ODNs treatment. Different clusters of genes associated with apoptosis, signal transduction, energy metabolism, lipid metabolism, etc., such as FXR1, PHLDA1, MAEA, PIK3R1, ICAM2, PITPN, CALM2, CAMK2D, PKIA, DRD2, SLC25A14, CKB, AADAC, LIPA, ACOX3, FADS1, were concerned. Analysis of differentially expressed genes will help to understand the effects of Y5 receptor antisense ODNs therapy.
Qiu
Jie
J
Department of Pediatrics, Nanjing Maternity and Child Health Hospital of Nanjing Medical University, 210004 Nanjing, China; Institute of Pediatrics of Nanjing Medical University, 210029 Nanjing, China.
Ni
Yu-Hui
YH
Chen
Rong-Hua
RH
Ji
Chen-Bo
CB
Liu
Feng
F
Zhang
Chun-Mei
CM
Gao
Chun-Lin
CL
Chen
Xiao-Hui
XH
Tong
Mei-Ling
ML
Chi
Xia
X
Zhou
Xiao-Yu
XY
Guo
Xi-Rong
XR
D052438:Adipocytes, White / Q000187:drug effects; D000273:Adipose Tissue / Q000378:metabolism*; D000818:Animals; D004435:Eating / Q000187:drug effects; D020869:Gene Expression Profiling; D007328:Insulin / Q000097:blood; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D016376:Oligonucleotides, Antisense / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D017476:Receptors, Neuropeptide Y / Q000235:genetics*; D020133:Reverse Transcriptase Polymerase Chain Reaction
D007328:Insulin; D016376:Oligonucleotides, Antisense; D017476:Receptors, Neuropeptide Y; C100568:neuropeptide Y5 receptor