Purification and characterization of cell suspensions peroxidase from cotton (Gossypium hirsutum L.).
Two peroxidases, cPOD-I and rPOD-II, have been isolated and purified from cotton cell suspension and their biochemical characteristics studied. rPOD-II from R405-2000, a non-embryogenic cultivar, has higher activity than cPOD-I derived from Coker 312, which developed an embryogenic structure. The cPOD-I and rPOD-II had molecular mass of 39.1 and 64 kDa respectively, as determined by SDS-PAGE. Both enzymes showed high efficiency of interaction with the guaiacol at 25 mM. The optimal pH for cPOD-I and rPOD-II activity was 5.0 and 6.0, respectively. The enzyme had an optimum temperature of 25 degrees C and was relatively stable at 20-30 degrees C. The isoenzymes were highly inhibited by ascorbic acid, dithiothreitol, sodium metabisulfite, and beta-mercaptoethanol. Their activities were highly enhanced by Al(3+), Fe(3+), Ca(2+), and Ni(2+), but they were moderately inhibited by Mn(2+) and K(+). The enzyme lost 50% to 62% of its activity in the presence of Zn(2+) and Hg(2+).
Kouakou
Tanoh Hilaire
TH
Laboratoire de Biologie et d'Amélioration des Productions Végétales, UFR Sciences de la Nature, Université d'Abobo-Adjamé, 02 BP 801, Abidjan 02, Ivory Coast. tanohilaire@yahoo.fr
Dué
Edmond Ahipo
EA
Kouadio
N'guessan Eugène Jean Parfait
NE
Niamké
Sébastien
S
Kouadio
Yatty Justin
YJ
Mérillon
Jean-Michel
JM
D000535:Aluminum / Q000494:pharmacology; D001205:Ascorbic Acid / Q000494:pharmacology; D002118:Calcium / Q000494:pharmacology; D002412:Cations / Q000494:pharmacology; D004229:Dithiothreitol / Q000494:pharmacology; D004789:Enzyme Activation / Q000187:drug effects; D004795:Enzyme Stability; D003368:Gossypium / Q000201:enzymology*; D006863:Hydrogen-Ion Concentration; D007501:Iron / Q000494:pharmacology; D007527:Isoenzymes / Q000302:isolation & purification* / Q000378:metabolism*; D008623:Mercaptoethanol / Q000494:pharmacology; D009532:Nickel / Q000494:pharmacology; D010544:Peroxidases / Q000302:isolation & purification* / Q000378:metabolism*; D013447:Sulfites / Q000494:pharmacology
D002412:Cations; D007527:Isoenzymes; D013447:Sulfites; C005200:sodium metabisulfite; D008623:Mercaptoethanol; D009532:Nickel; D000535:Aluminum; D007501:Iron; D010544:Peroxidases; D001205:Ascorbic Acid; D002118:Calcium; D004229:Dithiothreitol