title: Monitoring cellular movement in vivo with photoconvertible fluorescence protein "Kaede" transgenic mice.
abstract: Kaede is a photoconvertible fluorescence protein that changes from green to red upon exposure to violet light. The photoconversion of intracellular Kaede has no effect on cellular function. Using transgenic mice expressing the Kaede protein, we demonstrated that movement of cells with the photoconverted Kaede protein could be monitored from lymphoid organs to other tissues as well as from skin to the draining lymph node. Analysis of the kinetics of cellular movement revealed that each subset of cells in the lymph node, such as CD4(+) T, CD8(+) T, B, and dendritic cells, has a distinct migration pattern in vivo. Thus, the Kaede transgenic mouse system would be an ideal tool to monitor precise cellular movement in vivo at different stages of immune response to pathogens as well as in autoimmune diseases.
authors.affiliation: Laboratory for Autoimmune Regulation, Research Center for Allergy and Immunology, RIKEN, 1-7-22 Suehiro-cho, Tsurumi, Yokohama City, Kanagawa 230-0045, Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Tomura
authors.forename: Naoki
authors.initials: N
authors.lastname: Yoshida
authors.forename: Junko
authors.initials: J
authors.lastname: Tanaka
authors.forename: Satoshi
authors.initials: S
authors.lastname: Karasawa
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Miwa
authors.forename: Atsushi
authors.initials: A
authors.lastname: Miyawaki
authors.forename: Osami
authors.initials: O
authors.lastname: Kanagawa
mesh_terms: D000818:Animals; D002465:Cell Movement / Q000276:immunology*; D005434:Flow Cytometry; D005455:Fluorescent Antibody Technique; D006367:HeLa Cells; D006801:Humans; D007107:Immune System / Q000166:cytology*; D008164:Luminescent Proteins / Q000378:metabolism*; D051379:Mice; D008822:Mice, Transgenic
chemical_list: C513582:Kaede protein, Trachyphyllia geoffroyi; D008164:Luminescent Proteins