Glucose can reverse the effects of acute fasting on mouse ovulation and oocyte maturation.
Food deprivation suppresses ovulation. Although nutritional elements are responsible for this suppression, it is not clear whether energy metabolism has any effect on oocyte development under these circumstances. The aim of the present study was to determine which nutritional element is responsible for the effect of acute fasting on mouse ovulation and how oocyte development is affected. The results demonstrate that 64 h food deprivation blocks mouse ovulation. This was reversed by glucose feeding, oil feeding or short-term feeding, all of which elevated serum glucose levels. Furthermore, 48 h food deprivation inhibited follicle-stimulating hormone-induced oocyte maturation in vitro. However, 48 h glucose feeding increased serum glucose levels and restored oocyte maturation. Food deprivation increased serum progesterone levels and decreased serum oestradiol levels. Food deprivation also impaired follicle development, caused the death of oocytes and attenuated glucose consumption by cumulus-oocyte complexes. Taken together, the results indicate that: (1) the suppression of ovulation by acute fasting may be due to the control of oocyte development; and (2) maintaining serum glucose concentrations at a certain level is important for normal ovulation.
Yan
Jun
J
State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, PR China.
Zhou
Bo
B
Yang
Jie
J
Tai
Ping
P
Chen
Xiufen
X
Zhang
Hua
H
Zhang
Meijia
M
Xia
Guoliang
G
D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D004435:Eating / Q000502:physiology; D005215:Fasting / Q000009:adverse effects* / Q000097:blood; D005260:Female; D005508:Food Deprivation / Q000502:physiology; D005947:Glucose / Q000494:pharmacology*; D051379:Mice; D008815:Mice, Inbred Strains; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D009866:Oogenesis / Q000187:drug effects; D010060:Ovulation / Q000187:drug effects* / Q000502:physiology; D013997:Time Factors
D001786:Blood Glucose; D005947:Glucose