Construction of tissue-engineered cartilage with BMP7 gene-transfected chondrocytes.
In this study, the expression of bone morphogenetic protein-7 (BMP7) was investigated in tissue-engineered cartilage constructed using chondrocytes transfected with the BMP7 gene and that constructed using non-transfected chondrocytes after 7, 14, 21 and 28 days. The volume of the BMP7 gene-transfected tissue-engineered cartilage culture after 5 - 7 days was 9 x 9 x 2 mm, while that of the non-transfected tissue-engineered cartilage culture was 8 x 8 x 2 mm. Histomorphological analysis showed that both cultures comprised cartilaginous tissue. Both BMP7 mRNA and BMP7 protein were expressed in BMP7 gene-transfected cartilage culture grown in vitro for 7, 14, 21 or 28 days. The chondrocytes in the BMP7 gene-transfected cartilage culture were active, with increased mitochondria, Golgi bodies and rough endoplasmic reticulum compared with non-transfected cartilage. These results provide an ideal foundation for the study of BMP7 gene-transfected tissue-engineered cartilage transplantation in the repair of cartilaginous defects.
Qu
F J
FJ
The Second Affiliated Hospital of Harbin Medical University, Harbin, China.
Liu
Y
Y
Zhu
D L
DL
D000328:Adult; D000818:Animals; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins / Q000235:genetics* / Q000378:metabolism; D002356:Cartilage / Q000166:cytology* / Q000502:physiology; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000502:physiology*; D024043:Collagen Type II / Q000235:genetics / Q000378:metabolism; D006025:Glycosaminoglycans / Q000378:metabolism; D006801:Humans; D023822:Tissue Engineering; D016212:Transforming Growth Factor beta / Q000235:genetics* / Q000378:metabolism
C525819:BMP7 protein, human; D055419:Bone Morphogenetic Protein 7; D019485:Bone Morphogenetic Proteins; D024043:Collagen Type II; D006025:Glycosaminoglycans; D016212:Transforming Growth Factor beta