title: Laccase and lectin activities of intracellular proteins produced in a submerged culture of the xylotrophic basidiomycete Lentinus edodes.
abstract: The white-rot fungus Lentinus edodes produced D-melibiose-specific lectins and two laccase forms in a lignin-containing medium. The maxima of laccase and lectin activities coincided, falling within the period of active mycelial growth. The enzymes and lectins were isolated and purified by gel filtration followed by anion-exchange chromatography. The L. edodes lectins were found to be able to stabilize the activity of the fungus's own laccases. Lectin activity during the formation of lectin-enzyme complexes remained unchanged.
authors.affiliation: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia. elenavetrus@yandex.ru
authors.forename: Elena P
authors.initials: EP
authors.lastname: Vetchinkina
authors.forename: Natalia N
authors.initials: NN
authors.lastname: Pozdnyakova
authors.forename: Valentina E
authors.initials: VE
authors.lastname: Nikitina
mesh_terms: D002850:Chromatography, Gel; D002852:Chromatography, Ion Exchange; D003470:Culture Media; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D042845:Laccase / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D037102:Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D008553:Melibiose / Q000378:metabolism; D014442:Monophenol Monooxygenase / Q000378:metabolism; D025282:Mycelium / Q000254:growth & development; D020942:Shiitake Mushrooms / Q000201:enzymology* / Q000254:growth & development*
chemical_list: D003470:Culture Media; D005656:Fungal Proteins; D037102:Lectins; D008031:Lignin; D008553:Melibiose; D042845:Laccase; D014442:Monophenol Monooxygenase