[Differentially expressed genes in adrenal gland of H22 liver cancer mice with different syndromes and in different stages].
OBJECTIVE
To reveal the characteristics of gene expression in adrenal gland of H22 tumor mice with typical syndromes and in different liver cancer stages.


METHODS
By the quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array, we observed adrenal gland gene expression in H22 tumor mice with pathogenic factor-toxin predominance syndrome and qi deficiency syndrome in the earlier stage, yang-qi deficiency syndrome in the intermediate stage, and qi-yin-yang deficiency syndrome in the advanced stage. Genes highly expressed and remarkably different were analyzed in this study.


RESULTS
A total of seventy-three up-regulated coincident genes and twenty-six down-regulated coincident genes in different stages were investigated in the study. Up-regulated coincident genes included Hp, C3, Anxa1, Procr, C2, Il4ra, Cd14, Ptprc, Cd52, C4b, Eno3, Xdh, Gpx3, and so on. Down-regulated coincident genes included nervous system function-related genes such as Plp1, Mbp, Aldh1a1, Cck, Atn1, genes associated with electrolyte metabolism such as Aldh1a1 and Slc22a17, genes related to signal transduction such as Cxcr4, Spag5 and Stmn3, etc, and genes related to transcriptional control and protein biosynthesis such as Hspa1a, Dnajb1, Thra, Hhex and so on.


CONCLUSIONS
With the development of the tumorigenesis, the symptoms and signs and differentially expressed genes in adrenal gland of H22 tumor mice can be measured. Up-regulated and down-regulated coincident genes may be the features of H22 tumor mice different from those of normal mice.
Pan
Zhi-qiang
ZQ
School of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Fang
Zhao-qin
ZQ
Lu
Wen-li
WL
Liang
Chao
C
Wu
Zhong-hua
ZH
Liu
Xiao-mei
XM
Hou
Li
L
Zhang
Hui
H
Zhuo
Shao-yuan
SY
Liao
Ming-juan
MJ
Gao
Bi-feng
BF
D000311:Adrenal Glands / Q000378:metabolism*; D000818:Animals; D003937:Diagnosis, Differential; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D008114:Liver Neoplasms, Experimental / Q000235:genetics*; D008297:Male; D008516:Medicine, Chinese Traditional; D051379:Mice; D020411:Oligonucleotide Array Sequence Analysis; D011897:Random Allocation; D013577:Syndrome