[Study on antioxidant activity of constituents from mulberry leaf].
OBJECTIVE
To study the chemical constituents with antioxidant activity from mulberry leaf.


METHODS
Siliga gel column chromatography was used to isolate, and their structures were identified by spectras.


METHODS
Isolated constituents were tested in vitro for antioxidant activity against 1, 1-diphenyl -2-picrylhydrazyl (DPPH) and 2, 2' -azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS+) radicals.


RESULTS
Four known flavonoids were isolated from mulberry leaf and their structures were identified as kaempferol-3-O-beta-D-glucopyranoside (I), quercetin-3-O-beta-D-glucopyranoside (II), quercitrin (III), morin-3-O-beta-D-glucopyranoside (IV). All these compounds showed DPPH and ABTS+ radicals scavenging activity.


CONCLUSIONS
Compound IV has the strongest ABTS+ radicals scavenging effects.
Jiang
Yu-Lan
YL
College of Preclinical Medicine, Yanbian University, Yanji 133000, China.
Piao
Hui-Shan
HS
Li
Gao
G
D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005609:Free Radicals / Q000378:metabolism; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D031625:Morus / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology
D000975:Antioxidants; D005419:Flavonoids; D005609:Free Radicals; D009005:Monosaccharides; C517440:quercetin-3'-O-beta-D-glucopyranoside; C012526:quercitrin; D011794:Quercetin