title: Investigation of association between plasminogen activator inhibitor type-1 (PAI-1) gene 4G/5G polymorphism frequency and plasma PAI-1 enzyme activity in patients with acute stroke.
abstract: OBJECTIVE
  This study was carried out to determine if there is any association between plasminogen activator inhibitor type-1 (PAI-1) gene 4G/5G polymorphism and plasma PAI-1 enzyme activity in acute stroke patients.
  
  
  METHODS
  In this study, 333 genomic DNAs (from 253 acute stroke patients and 80 healthy subjects) were analyzed. Genomic DNAs were prepared from peripheral blood using a saline method. These DNAs were amplified by PCR method using primers specific for 4G and 5G alleles. PCR products were separated by 2% agarose gel electrophoresis and visualized by a charge coupled device (CCD) camera. PAI-1 enzyme activities were measured by ELISA method. The results were evaluated statistically with Student's t-test, chi(2)-test, one-way analysis of variance, and stepwise regression analysis.
  
  
  RESULTS
  In this study, frequency of PAI-1 gene 4G5G genotype was found to be low both in patients and controls. PAI-1 enzyme activities were significantly increased in acute stroke patients compared to controls. Although PAI-1 gene 4G5G genotype frequencies were low, the patients carrying this allele had highest plasma PAI-1 enzyme activity; likewise, although PAI-1 gene 4G4G genotype frequencies were high, the patients carrying this allele had lowest plasma PAI-1 enzyme activities. Homocysteine levels had a positive effect of 65% on plasma PAI-1 enzyme activities.
  
  
  CONCLUSIONS
  Consequently, in this study, we may assert that PAI-1 gene, 4G4G and 5G5G genotypes, PAI-1 activity, and homocysteine level determination are significant criteria for identifying patients who are likely to develop stroke; on the other hand, a direct relation does not exist between gene polymorphism and enzyme activity.
authors.affiliation: Department of Medical Biology, Medical Faculty, Eskisehir Osmangazi University, Eskisehir, Turkey.
authors.forename: Banu
authors.initials: B
authors.lastname: Kucukarabaci
authors.forename: Hasan Veysi
authors.initials: HV
authors.lastname: Gunes
authors.forename: Gazi
authors.initials: G
authors.lastname: Ozdemir
authors.forename: Didem
authors.initials: D
authors.lastname: Cosan
authors.forename: Demet
authors.initials: D
authors.lastname: Ozbabalik
authors.forename: Miris
authors.initials: M
authors.lastname: Dikmen
authors.forename: Irfan
authors.initials: I
authors.lastname: Degirmenci
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D006801:Humans; D008297:Male; D008875:Middle Aged; D017395:Plasminogen Activator Inhibitor 1 / Q000097:blood / Q000235:genetics*; D011110:Polymorphism, Genetic; D020521:Stroke / Q000235:genetics*; D010959:Tissue Plasminogen Activator / Q000235:genetics*
chemical_list: D017395:Plasminogen Activator Inhibitor 1; D010959:Tissue Plasminogen Activator