[Molecular analysis of some genes from plasmid p19 of the soil strain Bacillus subtilis 19 involved in conjugation].
Two fragments of conjugative plasmid p19 (95 kb) from the soil strain Bacillus subtilis 19 were cloned and sequenced; these fragments carry genes, products of which are indispensable for the conjugative transfer. One of the fragments 4518 bp in size carries five open reading frames and their fragments (ORF1-ORF5). The protein corresponding to ORF4 is homologous to proteins from the family VirD4. Inactivation of ORF4 and ORF1 by insertional mutagenesis caused a three-to-fivefold decrease in the frequency of plasmid p19 conjugative transfer. Another 2932-bp fragment of p19 was shown to possess a rep region homologous to the rep region of plasmid pBS72 from the soil strain B. subtilis 72 and a novel ORF (ORF6); the protein corresponding to this ORF contains the HTH motif typical for DNA-binding proteins.
Poluektova
E U
EU
Gagarina
E Iu
EIu
Shilovskiĭ
I P
IP
Fedorina
E A
EA
Nezametdinova
V Z
VZ
Rodionova
S A
SA
Prozorov
A A
AA
D001412:Bacillus subtilis / Q000235:genetics*; D003227:Conjugation, Genetic; D005798:Genes, Bacterial; D010957:Plasmids; D012988:Soil Microbiology