Immunocytochemical localization of TYMV-coded structural and nonstructural proteins by the protein A-gold technique.
Turnip yellow mosaic virus (TYMV) and its coat protein have been localized in ultrathin sections by an immunocytochemical technique using colloidal-gold labeled protein A as the marker. The high sensitivity of the technique and the good structural preservation allowed us to localize nonstructural proteins of the virus such as the RNA replicase and its viral-encoded subunit of 115 kDa.
Garnier
M
M
Laboratoire de Biologie Cellulaire et Moléculaire, INRA et Université de Bordeaux II, Domaine de la Grande Ferrade, 33140 Pont de la Maye, 33140 France.
Candresse
T
T
Bove
J M
JM