title: ESAT-6-specific CD4 T cell responses to aerosol Mycobacterium tuberculosis infection are initiated in the mediastinal lymph nodes.
abstract: CD4(+) T cell responses to aerosol Mycobacterium tuberculosis (Mtb) infection are characterized by the relatively delayed appearance of effector T cells in the lungs. This delay in the adaptive response is likely critical in allowing the bacteria to establish persistent infection. Because of limitations associated with the detection of low frequencies of naïve T cells, it had not been possible to precisely determine when and where naïve antigen-specific T cells are first activated. We have addressed this problem by using early secreted antigenic target 6 (ESAT-6)-specific transgenic CD4 T cells to monitor early T cell activation in vivo. By using an adoptive transfer approach, we directly show that T cell priming to ESAT-6 occurs only after 10 days of infection, is initially restricted to the mediastinal lymph nodes, and does not involve other lymph nodes or the lungs. Primed CD4 T cells rapidly differentiated into proliferating effector cells and ultimately acquired the ability to produce IFN-gamma and TNF-alpha ex vivo. Initiation of T cell priming was enhanced by two full days depending on the magnitude of the challenge inoculum, which suggests that antigen availability is a factor limiting the early CD4 T cell response. These data define a key period in the adaptive immune response to Mtb infection.
authors.affiliation: Trudeau Institute, 154 Algonquin Avenue, Saranac Lake, NY 12983, USA.
authors.forename: William W
authors.initials: WW
authors.lastname: Reiley
authors.forename: Mark D
authors.initials: MD
authors.lastname: Calayag
authors.forename: Susan T
authors.initials: ST
authors.lastname: Wittmer
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Huntington
authors.forename: John E
authors.initials: JE
authors.lastname: Pearl
authors.forename: Jeffrey J
authors.initials: JJ
authors.lastname: Fountain
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Martino
authors.forename: Alan D
authors.initials: AD
authors.lastname: Roberts
authors.forename: Andrea M
authors.initials: AM
authors.lastname: Cooper
authors.forename: Gary M
authors.initials: GM
authors.lastname: Winslow
authors.forename: David L
authors.initials: DL
authors.lastname: Woodland
mesh_terms: D019264:Adoptive Transfer; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology*; D005434:Flow Cytometry; D007700:Kinetics; D008198:Lymph Nodes / Q000276:immunology*; D008213:Lymphocyte Activation / Q000276:immunology; D008482:Mediastinum; D051379:Mice; D008822:Mice, Transgenic; D009169:Mycobacterium tuberculosis / Q000276:immunology*; D014376:Tuberculosis / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C093276:ESAT-6 protein, Mycobacterium tuberculosis