New variants of human papillomavirus type 18 identified in central Brazil.
HPV-18 is the second most prevalent human papillomavirus genotype found in cervical cancer. Nucleotide variations in HPV-18 sequence can interfere with the viral oncogenic potential. However, the knowledge about HPV-18 variants in Brazil is still limited. The present study aims to determine the LCR, E6, and L1 genetic variability of HPV-18 variants found in women co-infected with HIV-1 in Central Brazil. Four HPV-18 samples were identified and had the LCR, E6, and L1 genomic regions sequenced. It was possible to characterize three European variants and one African variant of HPV-18. All of them are new variants, showing nucleotide substitutions not previously reported. Nucleotide variations in binding sites for transcriptional factors were observed. Phylogenetic analysis was also performed, evidencing the three clusters related to the Asian-American, African, and European variants. The characterization of HPV genetic variability is of pivotal importance to the understanding of the viral pathogenicity.
Cerqueira
Daniela Marreco
DM
Pós-Graduação em Biologia Molecular, Instituto de Biologia, Universidade de Brasília ICC Sul, 70919-900, Brasília, DF, Brazil. danimarreco@yahoo.com.br
Raiol
Tainá
T
Véras
Nazle Mendonça Collaço
NM
von Gal Milanezi
Natália
N
Amaral
Fádia Aguiar
FA
de Macedo Brígido
Marcelo
M
Martins
Cláudia Renata Fernandes
CR
D001483:Base Sequence; D001938:Brazil; D005260:Female; D014644:Genetic Variation; D052161:Human papillomavirus 18 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D008969:Molecular Sequence Data; D010802:Phylogeny; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
D014764:Viral Proteins