Reciprocal relationship between expression of hypoxia inducible factor 1alpha (HIF-1alpha) and the pro-apoptotic protein bid in ex vivo colorectal cancer.
Hypoxia inducible factor 1 (HIF-1) represses the transcription of pro-apoptotic bid in colorectal cancer cells in vitro. To assess the clinical relevance of this observation, HIF-1alpha and Bid were assessed in serial sections of 39 human colorectal adenocarcinomas by immunohistochemistry. In high HIF-1alpha nuclear-positive cell subpopulations, there was a significant reduction in Bid expression (ANOVA, P=0.04). Given the role of Bid in drug-induced apoptosis, these data add impetus to strategies targeting HIF-1 for therapeutic gain.
Seenath
M M
MM
Clinical and Experimental Pharmacology, Paterson Institute of Cancer Research, Manchester, UK.
Roberts
D
D
Cawthorne
C
C
Saunders
M P
MP
Armstrong
G R
GR
O'Dwyer
S T
ST
Stratford
I J
IJ
Dive
C
C
Renehan
A G
AG
D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology; D000704:Analysis of Variance; D017209:Apoptosis; D051023:BH3 Interacting Domain Death Agonist Protein / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007150:Immunohistochemistry
D051023:BH3 Interacting Domain Death Agonist Protein; C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit