Escherichia coli constructs expressing human or porcine enterotoxins induce identical diarrheal diseases in a piglet infection model.
To develop a piglet model for studying diarrheal disease and developing vaccines, we challenged gnotobiotic piglets with isogenic Escherichia coli strains constructed to express porcine 987P(F6) fimbriae and a heat-labile or a heat-stable enterotoxin to examine clinical outcomes. Piglets developed identical diarrheal diseases when inoculated with constructs expressing human or porcine enterotoxins.
Zhang
Weiping
W
The Center for Infectious Disease Research & Vaccinology, South Dakota State University, Box 2175, North Campus Drive, Brookings, SD 57007, USA. weiping.zhang@sdstate.edu
Robertson
Donald C
DC
Zhang
Chengxian
C
Bai
Wei
W
Zhao
Mojun
M
Francis
David H
DH
D000818:Animals; D001427:Bacterial Toxins / Q000096:biosynthesis* / Q000235:genetics; D003967:Diarrhea / Q000382:microbiology* / Q000662:veterinary; D054307:Enterotoxigenic Escherichia coli / Q000235:genetics / Q000378:metabolism / Q000472:pathogenicity*; D004768:Enterotoxins / Q000096:biosynthesis* / Q000235:genetics; D004927:Escherichia coli Infections / Q000382:microbiology* / Q000662:veterinary; D029968:Escherichia coli Proteins / Q000096:biosynthesis* / Q000235:genetics; D010861:Fimbriae, Bacterial / Q000235:genetics; D015870:Gene Expression; D005798:Genes, Bacterial; D005856:Germ-Free Life; D054884:Host-Pathogen Interactions; D006801:Humans; D023421:Models, Animal; D010957:Plasmids; D013552:Swine / Q000382:microbiology; D013553:Swine Diseases / Q000382:microbiology*
D001427:Bacterial Toxins; D004768:Enterotoxins; D029968:Escherichia coli Proteins; C034151:heat stable toxin (E coli); C027538:heat-labile enterotoxin, E coli