title: Blast cells with nuclear extrusions in the form of micronuclei are associated with MYC amplification in acute myeloid leukemia.
abstract: We report three cases of acute myeloid leukemia without maturation [AML-M1 subtype according to the French-American-British classification (FAB)] with the presence of MYC oncogene amplification in form of double minutes (dmin) or homogeneously staining region (hsr). Blasts cells showed a particular morphology with extrusion of chromatin material. We observed by FISH the phenomenon of MYC aggregation in interphase cells and the formation of micronuclei excluded from the nucleus. The appearance of chromatin extrusion in cytological analysis should draw attention of the presence of dmin aggregation and possible MYC amplification.
authors.affiliation: Laboratori de Citogenètica i Biologia Molecular, Servei de Patologia. Hospital del Mar, URNHE, URTTS, PRBB-IMIM, Pg. Maritim 25-29, 08003 Barcelona, Spain.
authors.forename: Olaya
authors.initials: O
authors.lastname: Villa
authors.forename: Marta
authors.initials: M
authors.lastname: Salido
authors.forename: María Encarnación
authors.initials: ME
authors.lastname: Pérez-Vila
authors.forename: Ana
authors.initials: A
authors.lastname: Ferrer
authors.forename: Leonor
authors.initials: L
authors.lastname: Arenillas
authors.forename: Carmen
authors.initials: C
authors.lastname: Pedro
authors.forename: Blanca
authors.initials: B
authors.lastname: Espinet
authors.forename: Cristina
authors.initials: C
authors.lastname: Corzo
authors.forename: Sergi
authors.initials: S
authors.lastname: Serrano
authors.forename: Soledad
authors.initials: S
authors.lastname: Woessner
authors.forename: Lourdes
authors.initials: L
authors.lastname: Florensa
authors.forename: Francesc
authors.initials: F
authors.lastname: Solé
mesh_terms: D000368:Aged; D002467:Cell Nucleus / Q000235:genetics / Q000473:pathology*; D002843:Chromatin / Q000737:chemistry; D002869:Chromosome Aberrations; D020223:Chromosome Painting; D020732:Cytogenetic Analysis; D017809:Fatal Outcome; D005500:Follow-Up Studies; D005784:Gene Amplification; D016259:Genes, myc; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000097:blood* / Q000145:classification / Q000175:diagnosis / Q000235:genetics* / Q000473:pathology* / Q000628:therapy; D008297:Male; D008875:Middle Aged; D012074:Remission Induction; D013997:Time Factors; D014314:Trisomy
chemical_list: D002843:Chromatin