Effect of leukemia inhibitory factor (LIF) on the quality of in vitro produced mouse blastocysts and subsequent derivation of embryonic stem (ES) cells.
OBJECTIVE
To determine the effect of leukemia inhibitory factor (LIF) on the quality of in vitro produced mouse blastocyst and the efficiency of embryonic stem (ES) cell derivation.


METHODS
Experimental study


METHODS
Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University


METHODS
In vivo fertilized zygotes were collected and subjected to in vitro culture in potassium simplex optimized medium (KSOM) containing 1,000 unit/ml LIF. The developmental ability of the zygote to blastocyst-stage and the cell numbers in blastocysts were evaluated Expanded blastocysts developed in different culture media were subsequently subjected to ES cell derivation. MAIN OUTCOME MEASURE (s): The influence of LIF on the quality of and the total cell numbers of blastocyst developed in vitro.


RESULTS
Supplementation of LIF in KSOM increased the rate of hatching blastocysts (63.8% vs. 53.7%; p < 0.05) and total cell numbers (91.4 +/- 15.0 vs. 85.1 +/- 7.7; p < 0.05) compared to KSOM alone. ES cells were obtained 66.7% from blastocysts developed in KSOM-LIF versus 41.7% in KSOM (p > 0.05). Established ES cell lines showed typical colony and characteristics of pluripotent murine ES cells.


CONCLUSIONS
LIF improved the quality of in vitro produced blastocysts but not enhanced ES cell derivation.
Rungsiwiwut
Ruttachuk
R
Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
Rungarunlert
Sasitorn
S
Numchaisrika
Pranee
P
Pruksananonda
Kamthorn
K
Techakumphu
Mongkol
M
Virutamasen
Pramuan
P
D000818:Animals; D001755:Blastocyst; D047108:Embryonic Development; D053595:Embryonic Stem Cells; D005260:Female; D006801:Humans; D066298:In Vitro Techniques; D053605:Leukemia Inhibitory Factor; D051379:Mice; D015053:Zygote
D053605:Leukemia Inhibitory Factor