Identification of 30-kDa heat shock protein gene in Trichophyton rubrum.
Small heat shock proteins (sHSPs) are chaperones that are crucial in the heat shock response but also have important non-stress roles within the cell. HSP70 in Trichophyton rubrum is already detected and carefully characterised; however, no study was carried out for HSP30 in this pathogenic fungus. In the present study, T. rubrum was obtained from patients with dermatophytosis and cultured in appropriate conditions. High-molecular-weight DNA was extracted using standard extraction methods. Pairs of 21 nt primers were designed from highly conserved regions of the similar genes in other eukaryotic cells. Mentioned primers were utilised in PCR using isolated genomic DNA and extracted RNA templates of T. rubrum. The PCR fragments were then sequenced and 415 nucleotides of HSP30 in this pathogenic fungus were detected; the open reading frame had 156 nucleotides and was coding 51 amino acids. This gene (called TrHSP30) is registered in GenBank at National Center for Biotechnology Information (NIH, USA) database. Detection of TrHSP30 gene may open the way to determination of its possible role in the pathogenesis of dermatophyte infections due to T. rubrum.
Behzadi
Elham
E
Clinical Microbiology and Infectious Diseases Department, Faculty of Medicine, Abant Izzet Baysal University, Bolu, Turkey.
Behzadi
Payam
P
Sirmatel
Fatma
F
D000595:Amino Acid Sequence; D001483:Base Sequence; D003881:Dermatomycoses / Q000382:microbiology*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D050890:HSP30 Heat-Shock Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D008969:Molecular Sequence Data; D008970:Molecular Weight; D016415:Sequence Alignment; D014249:Trichophyton / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism
D005656:Fungal Proteins; D050890:HSP30 Heat-Shock Proteins