title: FRET imaging and in silico simulation: analysis of the signaling network of nerve growth factor-induced neuritogenesis.
abstract: Genetically encoded probes based on Förster resonance energy transfer (FRET) enable us to decipher spatiotemporal information encoded in complex tissues such as the brain. Firstly, this review focuses on FRET probes wherein both the donor and acceptor are fluorescence proteins and are incorporated into a single molecule, i.e. unimolecular probes. Advantages of these probes lie in their easy loading into cells, the simple acquisition of FRET images, and the clear evaluation of data. Next, we introduce our recent study which encompasses FRET imaging and in silico simulation. In nerve growth factor-induced neurite outgrowth in PC12 cells, we found positive and negative signaling feedback loops. We propose that these feedback loops determine neurite-budding sites. We would like to emphasize that it is now time to accelerate crossover research in neuroscience, optics, and computational biology.
authors.affiliation: Laboratory of Bioimaging and Cell Signaling, Graduate School of Biostudies, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan. tnakamr@lif.kyoto-u.ac.jp
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nakamura
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Aoki
authors.forename: Michiyuki
authors.initials: M
authors.lastname: Matsuda
mesh_terms: D000818:Animals; D031541:Fluorescence Resonance Energy Transfer / Q000379:methods*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D020932:Nerve Growth Factor / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D016501:Neurites / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D015398:Signal Transduction / Q000502:physiology*
chemical_list: D008164:Luminescent Proteins; D020932:Nerve Growth Factor