title: [Biotransformation of podophyllotoxin by cell suspension culture and root culture of Rheum palmatum].
abstract: OBJECTIVE
  To study the biotransformation of podophyllotoxin by the cell suspension culture and root culture systems of Rheum palmatum.
  
  
  METHODS
  Using plant tissue culture technology and HPLC techniques to isolate products. The structures were elucidated by spectroscopic means.
  
  
  RESULTS
  Cell suspension culture of R. palmatum could convert podophyllotoxin to produce picropodophyllotoxin with the yield of 73.8%, while root culture of R. palmatum could convert podophyllotoxin to produce epipodophyllotoxin and apopodophyllotoxin.
  
  
  CONCLUSIONS
  Podophyllotoxin did not affect the pH value of the media used in tissue cultures. Both cell suspension culture and root culture of R. palmatum can convert podophyllotoxin.
authors.affiliation: School of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
authors.forename: Ya-Jun
authors.initials: YJ
authors.lastname: Cui
authors.forename: Xiao-Feng
authors.initials: XF
authors.lastname: Liu
authors.forename: Jian
authors.initials: J
authors.lastname: Han
authors.forename: Bao-Rong
authors.initials: BR
authors.lastname: Wang
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
mesh_terms: D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D018517:Plant Roots / Q000378:metabolism*; D011034:Podophyllotoxin / Q000737:chemistry / Q000378:metabolism*; D012250:Rheum / Q000378:metabolism*; D046509:Tissue Culture Techniques / Q000379:methods*
chemical_list: D011034:Podophyllotoxin