Regulation of growth, fatty acid composition and delta 6 desaturase expression by dietary lipids in gilthead seabream larvae (Sparus aurata).
The Delta6 and Delta5 desaturases and elongases show only very limited activity in marine fish, and little is known of the possibility of enhancing Delta6 desaturase gene expression in these fish. The use of plant oils in marine fish diets is limited by their lack of n-3 highly unsaturated fatty acids (HUFA) despite an abundant content of the 18C fatty acid precursor linoleic and alpha-linolenic acids. The objective of the present study was to determine the ability of larval gilthead seabream to utilize vegetable oils and assess the nutritional regulation of Delta6 desaturase gene expression. Seventeen-day-old gilthead seabream larvae were fed during a 17-day period with one of four different microdiets formulated with either sardine fish oil (FO), soybean, rapeseed or linseed oils, respectively, or a fifth diet containing defatted squid meal and linseed oil. Good larval survival and growth, both in terms of total length and body weight, were obtained by feeding the larvae either rapeseed, soybean or linseed oils. The presence of vegetable oils in the diet increased the levels of 20:2n-9 and 20:2n-6, 18:2n-9, 18:3n-6, 20:3n-6 and 20:4n-6, in larvae fed rapeseed and soybean oils in comparison to those fed FO. In addition, a sixfold increase in the relative expression of Delta6 desaturase-like gene was found in larvae fed rapeseed and soybean oils, denoting the nutritional regulation of desaturase activity through its gene expression in this fish species. However, feeding linseed oil did not increase the expression of the Delta6 desaturase gene to such a high extent.
Izquierdo
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MS
Grupo de Investigación en Acuicultura, ICCM & IUSA, C/. Trasmontaña s/n, 35413, Arucas, Las Palmas, Spain.
Robaina
Lidia
L
Juárez-Carrillo
Eduardo
E
Oliva
V
V
Hernández-Cruz
Carmen María
CM
Afonso
Juan Manuel
JM
D000818:Animals; D004032:Diet / Q000662:veterinary*; D004042:Dietary Fats, Unsaturated / Q000008:administration & dosage / Q000494:pharmacology*; D005227:Fatty Acids / Q000032:analysis / Q000737:chemistry*; D005395:Fish Oils / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D007814:Larva / Q000187:drug effects / Q000201:enzymology / Q000235:genetics / Q000254:growth & development; D050602:Linoleoyl-CoA Desaturase / Q000235:genetics*; D010938:Plant Oils / Q000008:administration & dosage / Q000378:metabolism / Q000494:pharmacology; D021541:Sea Bream / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
D004042:Dietary Fats, Unsaturated; D005227:Fatty Acids; D005395:Fish Oils; D010938:Plant Oils; D050602:Linoleoyl-CoA Desaturase