Epitope mapping of human Fas using peptide phage display.
Sandwich EIA for measurement of soluble Fas was developed on the basis of SA-7 and SA-8 monoclonal antibodies to full-length human Fas. The threshold sensitivity of the test system is 0.3 ng/ml. Several isoforms of soluble Fas were identified. The structure of SA-7 and SA-8 antibody epitopes was determined using the peptide phage library. It was shown that SA-7 antibody epitope is determined by amino acid residues 129-134 (CKPNFF), while SA-8 antibody epitope is determined by amino acid residues 94-99 (KAHFSS) of full-length Fas. Hence, sandwich EIA on the basis of SA-7 and SA-8 monoclonal antibodies for detection of soluble Fas in human serum is to detect the following Fas isoforms: FasExo6Del, FasExo4Del, FasExo4,6Del, FasExo4.7Del, and FasExo8Del.
Abbasova
S G
SG
Affiliated Department of M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Organic Biochemistry, Russian Academy of Sciences, Moscow. svetlanaabbasova@rambler.ru
Shcheprova
Zh M
ZhM
Laman
A G
AG
Shepelyakovskaya
A O
AO
Baidakova
L K
LK
Rodionov
I L
IL
Kushlinskii
N E
NE
D018604:Epitope Mapping / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques; D019151:Peptide Library; D019014:fas Receptor / Q000276:immunology*
D019151:Peptide Library; D019014:fas Receptor