title: Construction of a versatile high precision ambient ionization source for direct analysis and imaging.
abstract: The design and construction of a high precision ambient ionization source matrix-assisted laser desorption electrospray ionization (MALDESI) are described in full detail, including a complete parts list. The computer controlled high precision motion control system and high repetition rate Explorer laser are demonstrated during MALDESI-FT-ICR analysis of peptides and proteins ranging from 1 to 17 kDa. The high stability ionization source platform described herein demonstrates both the advantages of the new MALDESI source and versatility for application to numerous desorption and ionization techniques.
authors.affiliation: W. M. Keck FT-ICR Mass Spectrometry Laboratory, Department of Chemistry, North Carolina State University, Raleigh, North Carolina 27695, USA.
authors.forename: Jason S
authors.initials: JS
authors.lastname: Sampson
authors.forename: Adam M
authors.initials: AM
authors.lastname: Hawkridge
authors.forename: David C
authors.initials: DC
authors.lastname: Muddiman
mesh_terms: D001920:Bradykinin / Q000737:chemistry; D002412:Cations / Q000737:chemistry*; D006801:Humans; D007834:Lasers; D008555:Melitten / Q000737:chemistry; D009113:Muramidase / Q000737:chemistry; D009211:Myoglobin / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000295:instrumentation* / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods; D025801:Ubiquitin / Q000737:chemistry
chemical_list: D002412:Cations; D009211:Myoglobin; D025801:Ubiquitin; D008555:Melitten; D009113:Muramidase; C514728:lysozyme C, human; D001920:Bradykinin