Effects of insulin treatment on HuC/HuD, NADH diaphorase, and nNOS-positive myoenteric neurons of the duodenum of adult rats with acute diabetes.
We carried out this investigation with the purpose of verifying whether insulin treatment prevents changes in the density of myoenteric neurons of the duodenum of Wistar rats with streptozotocin short-term diabetes. The animals from the diabetic group (D) lost more weight than the controls (group C), while the insulin treatment (group T) prevented weight loss in three animals and increased visceral fat in all of the animals of this group. Insulin treatment did not prevent the early loss of HuC/HuD myoenteric neurons. The density of nNOS-positive neurons did not change significantly in groups D and T. The density of NADHd-positive neurons in these groups was greater than in group C, indicating that short-term diabetes increases the activity of respiratory chain enzymes.
de Mello
Sônia Trannin
ST
Department of Morphophysiological Sciences, Laboratory of Enteric Neurons, State University of Maringá, Avenida Colombo, no. 5790 Bloco H-79, 87020-900, Maringá, PR, Brazil. stmello@uem.br
de Miranda Neto
Marcílio Hubner
MH
Zanoni
Jacqueline Nelisis
JN
Furlan
Maria Montserrat D P
MM
D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D008058:Dihydrolipoamide Dehydrogenase / Q000378:metabolism*; D004386:Duodenum / Q000294:innervation*; D007328:Insulin / Q000627:therapeutic use*; D008297:Male; D026602:Nitrergic Neurons / Q000187:drug effects*; D052248:Nitric Oxide Synthase Type I / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
D007328:Insulin; D052248:Nitric Oxide Synthase Type I; D008058:Dihydrolipoamide Dehydrogenase