Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex.
Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle.
Jasiak
Anna J
AJ
Gene Center and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig-Maximilians-University of Munich, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany.
Hartmann
Holger
H
Karakasili
Elena
E
Kalocsay
Marian
M
Flatley
Andrew
A
Kremmer
Elisabeth
E
Strässer
Katja
K
Martin
Dietmar E
DE
Söding
Johannes
J
Cramer
Patrick
P
D016681:Genome, Fungal / Q000502:physiology*; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II