Development of ChillPeach genomic tools and identification of cold-responsive genes in peach fruit.
The ChillPeach database was developed to facilitate identification of genes controlling chilling injury (CI), a global-scale post-harvest physiological disorder in peach. It contained 7,862 high-quality ESTs (comprising 4,468 unigenes) obtained from mesocarp tissues of two full-sib progeny contrasting for CI, about 48 and 13% of which are unique to Prunus and Arabidopsis, respectively. All ESTs are in the Gateway vector to facilitate functional assessment of the genes. The data set contained several putative SNPs and 184 unigenes with high quality SSRs, of which 42% were novel to Prunus. Microarray slides containing 4,261 ChillPeach unigenes were printed and used in a pilot experiment to identify differentially expressed genes in cold-treated compared to control mesocarp tissues, and in vegetative compared to mesocarp tissues. Quantitative RT-PCR (qRT-PCR) confirmed microarray results for all 13 genes tested. The microarray and qRT-PCR analyses indicated that ChillPeach is rich in putative fruit-specific and novel cold-induced genes. A website ( http://bioinfo.ibmcp.upv.es/genomics/ChillPeachDB ) was created holding detailed information on the ChillPeach database.
Ogundiwin
Ebenezer A
EA
Department of Plant Sciences, University of California, Davis, CA 93684, USA. ebenezer@uckac.edu
Martí
Cristina
C
Forment
Javier
J
Pons
Clara
C
Granell
Antonio
A
Gradziel
Thomas M
TM
Peace
Cameron P
CP
Crisosto
Carlos H
CH
D003080:Cold Temperature; D030541:Databases, Genetic; D020224:Expressed Sequence Tags; D005638:Fruit / Q000235:genetics*; D018506:Gene Expression Regulation, Plant; D015723:Gene Library; D017343:Genes, Plant; D023281:Genomics / Q000295:instrumentation*; D018895:Microsatellite Repeats / Q000235:genetics; D018598:Minisatellite Repeats / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D027861:Prunus / Q000235:genetics*; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D046888:Tissue Array Analysis