Enhancement of cell function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells.
An epithelial cell adhesion molecule, E-cadherin was expressed in NIH3T3 fibroblasts, and cell-cell interactions between keratinocytes and fibroblasts, or between hepatocytes and fibroblasts were artificially engineered. When the E-cadherin-expressing NIH3T3 cells were co-cultured with rat hepatocytes, the cell-cell contacts were formed with high frequency, and enhanced albumin secretion was observed.
Ito
Akira
A
Department of Chemical Engineering, Faculty of Engineering, Kyushu University, Fukuoka, Japan.
Kiyohara
Takehiko
T
Kawabe
Yoshinori
Y
Ijima
Hiroyuki
H
Kamihira
Masamichi
M
D000818:Animals; D015820:Cadherins / Q000378:metabolism*; D002450:Cell Communication / Q000502:physiology*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism*; D006801:Humans; D015603:Keratinocytes / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D041681:NIH 3T3 Cells; D011994:Recombinant Proteins / Q000378:metabolism
D015820:Cadherins; D011994:Recombinant Proteins