Physical maps of the genomes of dahlia mosaic virus and mirabilis mosaic virus-two members of the caulimovirus group.
The nucleic acids of dahlia mosaic virus (DaMV) and mirabilis mosaic virus (MMV) have been isolated and compared with the native DNA of cauliflower mosaic virus (CaMV). The native DNAs of these viruses separated into circular and linear molecules during gel electrophoresis to produce patterns nearly identical to those of CaMV. The DNAS of DaMV and MMV were cloned in bacteria and used for mapping the cleavage sites for 14 different restriction endonucleases. These sites were confirmed with native viral DNA. The S1 nuclease cleavage sites and the sizes of single-stranded denaturation products of the native DNA of each virus was used to determine the location of the four single-stranded interruptions present in each virus. The largest denaturation fragment of each virus migrated in gels at about the same rate as the a strand (which has one discontinuity) of CaMV. These features have been used to construct physical maps of the viral genomes.
Richins
R D
RD
Department of Plant Pathology, University of California, Davis, California 95616 , USA.
Shepherd
R J
RJ