In vivo volumetric imaging of vascular perfusion within human retina and choroids with optical micro-angiography.
Optical micro-angiography (OMAG), based on Fourier domain optical coherence tomography (OCT), is a recently developed imaging modality that provides dynamic blood flow imaging within microcirculation tissue beds in vivo. This paper presents its first application in imaging the blood circulations in posterior chamber of human eye. To eliminate/minimize the motion artifacts in OMAG flow image caused by the inevitable subject movement, we describe a method to compensate the bulk tissue motion by use of phase changes in sequential OCT A scan signals. By use of a fast OMAG/OCT imaging system at ~840nm wavelength band, we show that OMAG is capable of providing volumetric vasculatural images in retina and choroids, down to capillary level imaging resolution, within approximately 10 s. The depth-resolved volumetric views of the separate retina and choroid vasculatures are also presented. In the end of this paper, we provide a comparison of the OMAG results with those from Doppler OCT and optical coherence angiography.
An
Lin
L
Department of Biomedical Engineering, Oregon Health & Science University, Portland, OR 97237, USA.
Wang
Ruikang K
RK
D000792:Angiography / Q000379:methods*; D001783:Blood Flow Velocity; D002829:Choroid / Q000098:blood supply*; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D008833:Microcirculation / Q000166:cytology*; D055095:Optics and Photonics; D012171:Retinal Vessels / Q000166:cytology*; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000379:methods*