Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species.
By combining the photoswitching and localization of individual fluorophores with spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging with low crosstalk and down to 15 nm spatial resolution using only two detection color channels. The applicability of the method to biological specimens is demonstrated on mammalian cells. The combination of far-field fluorescence nanoscopy with the recording of a single switchable molecular species at a time opens up a new class of functional imaging techniques.
Bossi
Mariano
M
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Fölling
Jonas
J
Belov
Vladimir N
VN
Boyarskiy
Vadim P
VP
Medda
Rebecca
R
Egner
Alexander
A
Eggeling
Christian
C
Schönle
Andreas
A
Hell
Stefan W
SW
D000818:Animals; D002460:Cell Line; D003116:Color; D005456:Fluorescent Dyes / Q000737:chemistry*; D008853:Microscopy / Q000379:methods*; D015394:Molecular Structure
D005456:Fluorescent Dyes