BAC library construction and BAC end sequencing of five Drosophila species: the comparative map with the D. melanogaster genome.
We constructed and characterized arrayed bacterial artificial chromosome (BAC) libraries of five Drosophila species (D. melanogaster, D. simulans, D. sechellia, D. auraria, and D. ananassae), which are genetically well characterized in the studies of meiosis, evolution, population genetics, and developmental biology. The BAC libraries comprise 8,000 to 12,500 clones for each species, estimated to cover the most of the genomes. We sequenced both ends of most of these BAC clones with a success rate of 91%. Of these, 53,701 clones consisting of non-repetitive BAC end sequences (BESs) were mapped with reference of the public D. melanogaster genome sequences. The BES mapping estimated that the BAC libraries of D. auraria and D. ananassae covered 47% and 57% of the D. melanogaster genome, respectively, and those of D. melanogaster, D. sechellia, and D. simulans covered 94-97%. The low coverage by BESs of D. auraria and D. ananassae may be due to the high sequence divergence with D. melanogaster. From the comparative BES mapping, 111 possible breakpoints of chromosomal rearrangements were identified in these four species. The breakpoints of the major chromosome rearrangement between D. simulans and D. melanogaster on the third chromosome were determined within 20 kb in 84E and 30 kb in 93E/F. Corresponding breakpoints were also identified in D. sechellia. The BAC clones described here will be an important addition to the Drosophila genomic resources.
Murakami
Katsuhiko
K
RIKEN Genomic Sciences Center, Kanagawa, Japan.
Toyoda
Atsushi
A
Hattori
Masahira
M
Kuroki
Yoko
Y
Fujiyama
Asao
A
Kojima
Toshio
T
Matsuda
Muneo
M
Sakaki
Yoshiyuki
Y
Yamamoto
Masa-Toshi
MT
D000818:Animals; D002874:Chromosome Mapping; D022202:Chromosomes, Artificial, Bacterial; D004247:DNA / Q000737:chemistry; D004331:Drosophila melanogaster / Q000145:classification / Q000235:genetics*; D019143:Evolution, Molecular; D049750:Genome, Insect; D015698:Genomic Library; D010802:Phylogeny; D012091:Repetitive Sequences, Nucleic Acid; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D026801:Synteny
D004247:DNA