[Effect of salts on luminescence of natural and recombinant luminescent bacterial biosensors].
Effect of cations K+, Na+, Mg2+, and Ca2+ and anions SO4(2-), HCO3(-), and CO3(2-) on the luminescence intensity of the marine luminescent bacterium Photobacterium phorphoreum (Microbiosensor B-17 677f) and the recombinant strain Escherichia coli with cloned lux operon of P. leiognathi (Ekolyum-9). It is found that small concentrations of chlorides and sulfates of the cations studied had a concentration-dependent stimulatory effect on bacterial bioluminescence; as the concentration of agents increased, activation was succeeded by quenching. The strength of the inhibitory effect, which is characterized by EC50, decreased in the series Ca2+ > Na+ > Mg2+ > K+. Carbonates and hydrocarbonates had a pronounced inhibitory effect on the bioluminescence intensity, determined by an increase in pH. We showed that some types of highly mineralized water with a high hydrocarbonate content have a marked inhibitory effect on the luminescence intensity of microbial luminescent biosensors, mimicking the effect of chemical pollutants.
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D015374:Biosensing Techniques / Q000379:methods; D002412:Cations / Q000494:pharmacology; D002712:Chlorides / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D049453:Luciferases, Bacterial / Q000235:genetics / Q000378:metabolism*; D008163:Luminescent Measurements / Q000379:methods; D008670:Metals / Q000494:pharmacology; D009876:Operon / Q000502:physiology*; D010776:Photobacterium / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D013431:Sulfates / Q000494:pharmacology
D002412:Cations; D002712:Chlorides; D008670:Metals; D013431:Sulfates; D049453:Luciferases, Bacterial