Direct immobilization of cupredoxin azurin modified by site-directed mutagenesis on gold surface.
For making efficient bioelectronic device, we have developed novel immobilization method of cupredoxin azurin modified on gold (Au) surface. A recombinant protein with cysteine residue by using site-directed mutagenesis was designed and then directly immobilized on Au surface without any chemical linker. The immobilization of the functionalized protein is confirmed by surface plasmon resonance (SPR) and its surface morphology is analyzed by scanning tunneling microscopy (STM). The immobilization efficiency has been increased about 75.6%, as compared to that of wild-type azurin. The electrochemical property of the fabricated thin film was investigated by the cyclic voltammetry (CV). As a result, cysteine-modified azurin can be used for making high-quality protein film, and applied to the fabrication of nano-scale bioelectronics.
Kim
Sang-Uk
SU
Department of Chemical & Biomolecular Engineering, Sogang University, Seoul 121-742, Republic of Korea.
Kim
Young Jun
YJ
Choi
Shin-Geon
SG
Yea
Cheol-Heon
CH
Singh
Ravindra P
RP
Min
Junhong
J
Oh
Byung-Keun
BK
Choi
Jeong-Woo
JW
D000327:Adsorption; D001400:Azurin / Q000737:chemistry / Q000235:genetics; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry*; D016252:Microscopy, Scanning Tunneling / Q000379:methods*; D016297:Mutagenesis, Site-Directed / Q000379:methods*; D011550:Pseudomonas aeruginosa / Q000201:enzymology / Q000235:genetics; D020349:Surface Plasmon Resonance / Q000379:methods*
C050107:cupredoxin; D001400:Azurin; D006046:Gold