Early induction of moderate hypothermia suppresses systemic inflammatory cytokines and intracellular adhesion molecule-1 in rats with caerulein-induced pancreatitis and endotoxemia.
OBJECTIVE
To evaluate the systemic effects of moderate hypothermia (MH) and the timing of induction on acute pancreatitis (AP) and endotoxemia in rats.


METHODS
The effects of MH were compared in 4 groups, that is, sham group (38 degrees C), control group (38 degrees C), early MH group (32 degrees C on administration of lipopolysaccharide [LPS]), and delayed MH group (32 degrees C 1 hour after LPS). AP and endotoxemia were induced by intramuscular injection of caerulein and intraperitoneal injection of LPS.


RESULTS
Serum interleukin 6 (IL-6) in both MH groups was significantly lower than that in the control group at 3 hours. Serum interleukin 10 (IL-10) in the early MH group was significantly higher than those in the other 3 groups at 1 hour. IL-10/IL-6 ratios in both MH groups were significantly higher than that in the control group at 3 hours. Serum soluble intercellular adhesion molecule (sICAM-1) in both MH groups was significantly lower than that in the control group at 3 hours. Serum sICAM-1 in the early MH group was significantly lower than that in the delayed MH group. The tendency of pancreatic ICAM-1 was similar to that of serum sICAM-1.


CONCLUSIONS
Early induction of MH might be protective against pancreatic injury and systemic inflammation in AP and endotoxemia.
Fujimoto
Kenji
K
Department of Critical Care and Emergency Medicine, Yamaguchi University School of Medicine, Ube, Yamaguchi, Japan. fujiken-ygc@umin.ac.jp
Fujita
Motoki
M
Tsuruta
Ryosuke
R
Tanaka
Ryo
R
Shinagawa
Hiromi
H
Izumi
Tomonori
T
Kasaoka
Shunji
S
Maekawa
Tsuyoshi
T
D000818:Animals; D002108:Ceruletide / Q000633:toxicity; D016207:Cytokines / Q000097:blood*; D019446:Endotoxemia / Q000097:blood* / Q000139:chemically induced / Q000628:therapy*; D007036:Hypothermia, Induced; D018836:Inflammation Mediators / Q000097:blood*; D018799:Intercellular Adhesion Molecule-1 / Q000097:blood* / Q000378:metabolism; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008070:Lipopolysaccharides / Q000633:toxicity; D008297:Male; D010179:Pancreas / Q000378:metabolism; D010195:Pancreatitis / Q000097:blood* / Q000139:chemically induced / Q000628:therapy*; D051381:Rats; D017208:Rats, Wistar
D016207:Cytokines; D018836:Inflammation Mediators; D015850:Interleukin-6; D008070:Lipopolysaccharides; D018799:Intercellular Adhesion Molecule-1; D016753:Interleukin-10; D002108:Ceruletide