Genome organization of Treponema zioleckii.
Genome analysis of Treponema zioleckii proved that, in this bacterium, besides chromosomal DNA, a relatively small extrachromosomal DNA element is present. This element was shown to be a double-stranded circular plasmid DNA of approximately 7 kbp; it was designated as pKT. The plasmid was characterized by molecular and bioinformatic analysis. No pKT homologous DNA sequences were detected in other rumen Treponema strains. The overall G+C content of the pKT plasmid is approximately 56 %, which is higher than in other Treponema plasmids or genomes. The Rep module of the pKT plasmid consisting of the rep gene and the region of repeats was identified within a 1.6-kbp fragment. The putative rep gene encodes the replication protein belonging to the pfam04796 RepA_C family of proteins with the highest similarity (25 % within 249 amino acids) to the RepA protein from the green sulfur bacterium Prosthecochloris aestuarii.
Sikorová
L
L
Institute of Animal Physiology, Slovak Academy of Sciences, 040 01 Kosice, Slovakia. luciannaa@pobox.sk
Piknová
M
M
Javorský
P
P
Pristas
P
P
D000595:Amino Acid Sequence; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics; D001482:Base Composition; D004261:DNA Replication; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D016680:Genome, Bacterial; D008969:Molecular Sequence Data; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D010957:Plasmids / Q000235:genetics; D012417:Rumen / Q000382:microbiology; D017422:Sequence Analysis, DNA; D012756:Sheep; D014208:Treponema / Q000235:genetics* / Q000302:isolation & purification
D001426:Bacterial Proteins; D004269:DNA, Bacterial