Incorporation of Y'-Ty1 cDNA destabilizes telomeres in Saccharomyces cerevisiae telomerase-negative mutants.
Ty1 retrotransposons in Saccharomyces cerevisiae are activated by telomere erosion. Ty1-dependent reverse transcription of mRNA from subtelomeric Y' repeats generates chimeric Y'-Ty1 cDNA. Here, we show that Y'-Ty1 cDNA is incorporated at eroding telomeres in the absence of telomerase. Telomeric incorporation of Y'-Ty1 cDNA promotes genome rearrangements.
Maxwell
Patrick H
PH
Laboratory of Developmental Genetics, Wadsworth Center and Department of Biomedical Sciences, University at Albany School of Public Health, Albany, New York 12201-2002, USA.
Curcio
M Joan
MJ
D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism*; D004271:DNA, Fungal / Q000235:genetics / Q000378:metabolism; D015321:Gene Rearrangement; D008957:Models, Genetic; D009154:Mutation; D018626:Retroelements / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D019098:Telomerase / Q000235:genetics*; D016615:Telomere / Q000378:metabolism*
D018076:DNA, Complementary; D004271:DNA, Fungal; D018626:Retroelements; D019098:Telomerase