Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry fingerprinting.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral 'fingerprints' for twelve Penicillium species. Prior to MALDI-TOF MS analysis, eight replicate cultures of each Penicillium species were subjected to three one-minute bead-beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contained abundant peaks in the range of m/z 5000-20 000, and allowed unambiguous discrimination between species. In addition, a biomarker common to all Penicillium mass spectra was observed at m/z 13 900. Discriminant analysis using the MALDI-TOF MS data yielded classification error rates of 0% (i.e. 100% correct identification), indicating that MALDI-TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance.
Hettick
Justin M
JM
Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505, USA. jhettick@cdc.gov
Green
Brett J
BJ
Buskirk
Amanda D
AD
Kashon
Michael L
ML
Slaven
James E
JE
Janotka
Erika
E
Blachere
Francoise M
FM
Schmechel
Detlef
D
Beezhold
Donald H
DH
D016000:Cluster Analysis; D005656:Fungal Proteins / Q000737:chemistry*; D010407:Penicillium / Q000302:isolation & purification / Q000378:metabolism*; D010449:Peptide Mapping; D015203:Reproducibility of Results; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*
D005656:Fungal Proteins