Differential immunological phenotypes are exhibited after scald and flame burns.
A dysfunctional immune system is known to be part of the pathophysiology after burn trauma. However, reports that support this have used a variety of methods, with numerous variables, to induce thermal injury. We hypothesized that, all other parameters being equal, an injury infliction by a scald would yield different immunological responses than one inflicted by a flame. Here, we demonstrated that both burn methods produced a full-thickness burn, yet there was more of an increase in subdermal temperature, hematocrit, mortality, and serum IL-6 concentrations associated with the scald burn. On postinjury day 1, the scald-burned mice showed diminished lymphocyte numbers, interferon gamma production, and lymphocyte T-bet expression as compared with sham- and flame-burned mice. On postburn day 8, spleens from both sets of thermally injured animals showed an increase in proinflammatory myeloid cells as compared with sham-burned mice. Furthermore, the T-cell numbers, T-bet expression, and phenotype were changed such that interferon gamma production was higher in scald-burned mice than in sham- and flame-burned mice. Altogether, the data show that differential immunological phenotypes were observed depending on the thermal injury method used.
Tschöp
Johannes
J
Department of Research, Shriners Hospital for Children, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267-0558, USA.
Martignoni
André
A
Reid
Maria D
MD
Adediran
Samuel G
SG
Gardner
Jason
J
Noel
Greg J
GJ
Ogle
Cora K
CK
Neely
Alice N
AN
Caldwell
Charles C
CC
D000818:Animals; D002056:Burns / Q000276:immunology* / Q000628:therapy*; D016207:Cytokines / Q000378:metabolism; D005434:Flow Cytometry; D006400:Hematocrit; D007249:Inflammation; D007371:Interferon-gamma / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D008264:Macrophages / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010641:Phenotype; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000096:biosynthesis*; D013601:T-Lymphocytes / Q000378:metabolism; D000098815:T-bet Transcription Factor
D016207:Cytokines; D015850:Interleukin-6; D020825:T-Box Domain Proteins; D000098815:T-bet Transcription Factor; D007371:Interferon-gamma