title: Entrapment of ionic tris(2,2'-bipyridyl) ruthenium(II) in hydrophobic siliceous zeolite: O2 sensing in biological environments.
abstract: Synthesis of the ionic dye, tris(2,2'-bipyridyl) ruthenium(II) chloride (Ru(bpy) 3 2+.2Cl (-)) within the supercages of a highly hydrophobic zeolite Y is reported. Use of the neutral precursor Ru(bpy)Cl 2(CO) 2 allowed for high loading levels of Ru(bpy) 3 2+ (1 per 7 and 25 supercages). The emission quenching of the Ru(bpy) 3 2+-zeolite crystals dispersed in polydimethoxysiloxane (PDMS) films by dissolved oxygen in water was examined. The quenching data as a function of oxygen concentration was fit to a linear Stern-Volmer plot ( R2 = 0.98). Using the Stern-Volmer plot as calibration, changes in concentration of dissolved oxygen due to reaction with glucose in the presence of glucose oxidase was monitored. Human monocyte-derived macrophages internalized the submicron-sized Ru(bpy) 3 2+-zeolite crystals, and intracellular oxygen concentrations initiated by zymosan-mediated oxidative burst could be monitored by measuring the emission from Ru(bpy) 3 2+ by confocal fluorescence microscopy.
authors.affiliation: Department of Chemistry, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: Toni A
authors.initials: TA
authors.lastname: Ruda-Eberenz
authors.forename: Amber
authors.initials: A
authors.lastname: Nagy
authors.forename: W James
authors.initials: WJ
authors.lastname: Waldman
authors.forename: Prabir K
authors.initials: PK
authors.lastname: Dutta
mesh_terms: D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007477:Ions / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D008855:Microscopy, Electron, Scanning; D015394:Molecular Structure; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D009942:Organometallic Compounds / Q000737:chemistry*; D010100:Oxygen / Q000737:chemistry*; D017655:Silicon Compounds / Q000737:chemistry*; D017641:Zeolites / Q000737:chemistry*
chemical_list: D007477:Ions; D009942:Organometallic Compounds; D017655:Silicon Compounds; C415768:tris(2,2'-bipyridyl)ruthenium(II); D017641:Zeolites; D010100:Oxygen