Association of cocaine- and amphetamine-regulated transcript (CART) messenger RNA level, food intake, and growth in channel catfish.
Cocaine-and Amphetamine-Regulated Transcript (CART) is a potent hypothalamic anorectic peptide in mammals and fish. We hypothesized that increased food intake is associated with changes in expression of CART mRNA within the brain of channel catfish. Objectives were to clone the CART gene, examine tissue CART mRNA distribution, and changes in the amount of CART mRNA in relation to changes in food intake in channel catfish. Our results showed that channel catfish CART was highly similar to those of other fish species, particularly in the biologically active portion of the peptide. Expression of CART mRNA was detected in the brain and testis but not in other somatic tissues. Thirty days of fasting decreased (P<0.05) the amount of CART mRNA within the brain of channel catfish, while refeeding for 15 days restored its amount to a level similar to the fed control. In a separate 7 week feeding study, CART mRNA expression was lower in fish that consumed more food and gained more weight (P<0.05). These results suggest that CART is involved in regulation of food intake in channel catfish, similarly as it has been reported in other fish and mammals.
Kobayashi
Yasuhiro
Y
Division of Biological and Physical Sciences, Delta State University, PO Box 3262, Cleveland, MS 38733, USA. kobayashi@deltastate.edu
Peterson
Brian C
BC
Waldbieser
Geoffrey C
GC
D000818:Animals; D001483:Base Sequence; D001921:Brain / Q000378:metabolism*; D004435:Eating / Q000502:physiology*; D005786:Gene Expression Regulation / Q000235:genetics / Q000502:physiology; D007031:Hypothalamus / Q000502:physiology*; D007059:Ictaluridae / Q000254:growth & development / Q000378:metabolism; D008297:Male; D008969:Molecular Sequence Data; D009419:Nerve Tissue Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000032:analysis / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013045:Species Specificity; D013997:Time Factors; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein
D009419:Nerve Tissue Proteins; D012333:RNA, Messenger; D000098882:Cocaine- and Amphetamine-Regulated Transcript Protein