title: Human myoblasts modulate the function of antigen-presenting cells.
abstract: Muscle biopsy specimens of myositis patients were analyzed for the presence of dendritic cells (DC) and macrophages (MPh) by immunohistochemistry. The interaction of DC and myoblasts (MB) was studied by coculture and effects on DC phenotype and function were assessed by flow cytometry and T-cell proliferation assays. Effects of MB-lysates on the phagocytic capacity of MPh were analyzed in bead-incorporation assays. Myositis specimens revealed a tendency towards more immature DC. MB modulated the maturation state of DC and DC recovered from MB-coculture had an inhibitory effect on T-cell proliferation. MB-lysates strongly stimulated MPh phagocytosis. Hypothetically, MB might modulate APC, counterbalancing immune-mediated damage.
authors.affiliation: Department of Neurology, University of Wuerzburg, Germany.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Schwab
authors.forename: Anne
authors.initials: A
authors.lastname: Waschbisch
authors.forename: Barbara
authors.initials: B
authors.lastname: Wrobel
authors.forename: Hanns
authors.initials: H
authors.lastname: Lochmüller
authors.forename: Claudia
authors.initials: C
authors.lastname: Sommer
authors.forename: Heinz
authors.initials: H
authors.lastname: Wiendl
mesh_terms: D018528:ATP-Binding Cassette Transporters / Q000378:metabolism; D015703:Antigens, CD / Q000378:metabolism; D015815:Cell Adhesion Molecules / Q000378:metabolism; D002478:Cells, Cultured; D018920:Coculture Techniques / Q000379:methods; D003713:Dendritic Cells / Q000378:metabolism*; D005434:Flow Cytometry; D015395:Histocompatibility Antigens Class I / Q000378:metabolism; D006801:Humans; D037181:Lectins, C-Type / Q000378:metabolism; D008213:Lymphocyte Activation / Q000276:immunology; D051907:Lysosomal Membrane Proteins / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D032448:Myoblasts, Skeletal / Q000473:pathology / Q000502:physiology*; D009220:Myositis / Q000473:pathology / Q000503:physiopathology; D011956:Receptors, Cell Surface / Q000378:metabolism; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin
chemical_list: D018528:ATP-Binding Cassette Transporters; D015703:Antigens, CD; D015815:Cell Adhesion Molecules; D015395:Histocompatibility Antigens Class I; D037181:Lectins, C-Type; D051907:Lysosomal Membrane Proteins; D011956:Receptors, Cell Surface; D000099215:DC-Specific ICAM-3 Grabbing Nonintegrin; C435500:ABCC11 protein, human