Serum xylosyltransferase activity in diabetic patients as a possible marker of reduced proteoglycan biosynthesis.
OBJECTIVE
Proteoglycan metabolism is altered in diabetic patients. The xylosyltransferases (XTs) are the initial and rate-limiting enzymes in the biosynthesis of the glycosaminoglycan chains in proteoglycans. Here, we analyzed whether the changed proteoglycan metabolism leads to altered serum XT levels in diabetic patients.


METHODS
Serum XT activity was determined in 100 diabetic patients and 100 blood donors using a novel high-performance liquid chromatography electrospray ionization tandem mass spectrometry assay.


RESULTS
Serum XT activities in male and female diabetic patients were significantly decreased compared with those in the corresponding normoglycemic control subjects (mean +/- SD: male patients, 19.3 +/- 4.44 mU/l; male nondiabetic control subjects, 26.6 +/- 2.79 mU/l; female patients, 18.9 +/- 3.14 mU/l; female nondiabetic control subjects, 21.8 +/- 3.74 mU/l; P < 0.0001). No significant differences were detected between patients with type 1 and type 2 diabetes.


CONCLUSIONS
Our data show decreased XT activity in patients with diabetes, a disease that is accompanied by an altered proteoglycan biosynthesis.
Götting
Christian
C
Institut für Laboratoriums-und Transfusionsmedizin, Herz-und Diabeteszentrum Nordrhein-Westfalen, Universitätsklinik der Ruhr-Universität Bochum, Bad Oeynhausen, Germany. cgoetting@hdz-nrw.de
Kuhn
Joachim
J
Kleesiek
Knut
K
D000328:Adult; D017668:Age of Onset; D000368:Aged; D015415:Biomarkers; D001782:Blood Donors; D015331:Cohort Studies; D003920:Diabetes Mellitus / Q000097:blood* / Q000201:enzymology*; D003922:Diabetes Mellitus, Type 1 / Q000097:blood / Q000201:enzymology; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000201:enzymology; D005260:Female; D006442:Glycated Hemoglobin / Q000378:metabolism; D006025:Glycosaminoglycans / Q000096:biosynthesis; D006801:Humans; D008297:Male; D008875:Middle Aged; D010430:Pentosyltransferases / Q000097:blood*; D011509:Proteoglycans / Q000096:biosynthesis*; D012016:Reference Values; D000097608:UDP Xylose-Protein Xylosyltransferase
D015415:Biomarkers; D006442:Glycated Hemoglobin; D006025:Glycosaminoglycans; D010430:Pentosyltransferases; D011509:Proteoglycans; D000097608:UDP Xylose-Protein Xylosyltransferase