title: Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography.
abstract: P-glycoprotein (ABCB1) is an ATP-binding cassette protein that is associated with the acquisition of multi-drug resistance in cancer and the failure of chemotherapy in humans. Structural insights into this protein are described using a combination of small angle X-ray scattering data and cryo-electron crystallography data. We have compared the structures with bacterial homologues, and discuss the development of homology models for P-glycoprotein based on the bacterial Sav1866 structure.
authors.affiliation: Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: McDevitt
authors.forename: Chitra A
authors.initials: CA
authors.lastname: Shintre
authors.forename: J Günter
authors.initials: JG
authors.lastname: Grossmann
authors.forename: Naomi L
authors.initials: NL
authors.lastname: Pollock
authors.forename: Stephen M
authors.initials: SM
authors.lastname: Prince
authors.forename: Richard
authors.initials: R
authors.lastname: Callaghan
authors.forename: Robert C
authors.initials: RC
authors.lastname: Ford
mesh_terms: D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D020285:Cryoelectron Microscopy; D003461:Crystallography; D004583:Electrons; D006801:Humans; D011487:Protein Conformation; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001426:Bacterial Proteins