Effect of mitomycin in the surgical treatment of tracheal stenosis.
OBJECTIVE
To assess the capacity of high and low doses of the antimitotic drug mitomycin to prevent laryngeal stenosis in an animal model.


METHODS
A prospective, randomized, double-blind, controlled study was carried out. End-to-end anastomosis was performed in 18 rabbits after tracheal annulus resection to produce inflammation. There were 3 treatment groups: topical saline (isotonic sodium chloride solution) and low-dose (0.2 mg/mL) and high-dose (0.5 mg/mL) topical mitomycin.


RESULTS
A total of 107 procedures were performed: 54 surgical procedures, 35 fibrobronchoscopies, and 18 biopsies. The effect of mitomycin was dose related. In the high-dose mitomycin group, most rabbits progressed to stenosis with a percentage decrease in airway diameter that was significantly greater than in the other 2 groups (P <.001). The mean (SD) percentage of maximum stenosis in the high-dose group was 51% (22%). In the low-dose and saline groups, it was 18% (13%) and 16% (9%), respectively. No significant differences in tracheal stenosis between the low-dose mitomycin and saline groups were observed. Blinded histopathological analysis also showed no significant differences between the saline group and the low-dose mitomycin group. Compared with the other 2 groups, the high-dose mitomycin group had a significant increase in fibroproliferative tissue (P <.001).


CONCLUSIONS
These results suggest that topical mitomycin is not effective for avoiding tracheal stenosis and may provoke the opposite effect if the dose is not carefully selected.
Iñiguez-Cuadra
Rodrigo
R
Department of Otolaryngology-Head and Neck Surgery, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile. riniguez@uc.cl
San Martín Prieto
José
J
Iñiguez-Cuadra
Magaly
M
Zúñiga Erranz
Sergio
S
Jofré Pavez
David
D
González Bombardiere
Sergio
S
Guilemany Toste
J M
JM
Iñiguez-Sasso
Rodrigo
R
D000402:Airway Obstruction / Q000473:pathology / Q000517:prevention & control; D000714:Anastomosis, Surgical; D000818:Animals; D050256:Antimitotic Agents / Q000494:pharmacology*; D001706:Biopsy; D001999:Bronchoscopy; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007828:Laryngoscopy; D007829:Laryngostenosis / Q000473:pathology / Q000517:prevention & control*; D007830:Larynx / Q000473:pathology / Q000601:surgery; D016685:Mitomycin / Q000494:pharmacology*; D011183:Postoperative Complications / Q000473:pathology / Q000517:prevention & control*; D011446:Prospective Studies; D011817:Rabbits; D014132:Trachea / Q000473:pathology / Q000601:surgery*; D014135:Tracheal Stenosis / Q000473:pathology / Q000517:prevention & control*
D050256:Antimitotic Agents; D016685:Mitomycin