Influence of heat stress on the cortisol and oxidant-antioxidants balance during oestrous phase in buffalo-cows (Bubalus bubalis): thermo-protective role of antioxidant treatment.
In the present study, the effect of heat stress, which is commonly observed in the animals of Upper Egypt area in summer, as well as the effect of antioxidant treatment as a thermo-protective was examined. In this study, the animals (n = 120) were divided into winter group (n = 40, bred during winter) and summer group (n = 80, bred during summer) as well as, animals in the summer group were divided into first subgroup animals (n = 40) and injected with Viteselen intramuscularly (15 ml) twice weekly for 10 weeks and second subgroup animals (n = 40) were not treated (as control). Serum levels of progesterone (P4), oestradiol (E2), cortisol, superoxide dismutase (SOD), lipid peroxidase (LPO) and nitric oxide (NO) were measured. The pregnancy rate of all animals was detected rectally. The levels of oestradiol and the activity of the antioxidant SOD were decreased in serum of animals in behavioural oestrus during summer as compared with those in winter. During the same time period the levels of oxidants such as LPO and NO were increased in the serum of animals again in the phase of oestrus. In another group of animals treated by intramuscular injection with 15 ml viteselen (antioxidant) twice weekly for 6 weeks during hot months, the activities of serum SOD showed an increase and the levels of oxidants and cortisol decreased. Moreover, the levels of oestradiol were increased during the oestrous behaviour. The pregnancy rate was decreased in animals under heat stress and the pregnancy rate was enhanced dramatically when these animals received antioxidants during the heat stress. This means that the heat-stress in Upper Egypt may affect the fertility of animals and pregnancy rate and this effect may be through an increased production of free radicals and decreased production of antioxidants as well as increased levels of cortisol. Treatment of animals or supplementation with antioxidants before the beginning of months of heat-stress and also during the stress period may correct the infertility due to heat-stress through the decrease in cortisol secretion and a decrease in the oxidative stress. These results resulted in an increase in pregnancy rate in treated animals.
Megahed
G A
GA
Department of Theriogenology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt.
Anwar
M M
MM
Wasfy
S I
SI
Hammadeh
M E
ME
D000818:Animals; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D002020:Buffaloes / Q000097:blood / Q000378:metabolism* / Q000502:physiology; D004958:Estradiol / Q000097:blood; D004971:Estrus / Q000378:metabolism*; D005260:Female; D018882:Heat Stress Disorders / Q000097:blood / Q000517:prevention & control / Q000662:veterinary*; D006854:Hydrocortisone / Q000097:blood*; D007273:Injections, Intramuscular / Q000662:veterinary; D015227:Lipid Peroxidation / Q000187:drug effects; D009569:Nitric Oxide / Q000097:blood / Q000378:metabolism; D010084:Oxidation-Reduction; D011247:Pregnancy; D018873:Pregnancy Rate; D011374:Progesterone / Q000097:blood; D012621:Seasons; D013482:Superoxide Dismutase / Q000097:blood / Q000187:drug effects / Q000378:metabolism
D000975:Antioxidants; D009569:Nitric Oxide; D011374:Progesterone; D004958:Estradiol; D013482:Superoxide Dismutase; D006854:Hydrocortisone