title: Isolation of oryzanol from crude rice bran oil.
abstract: The isolation of oryzanol from crude rice bran oil (RBO) was achieved by a two-step crystallization process. In the first crystallization, oryzanol was concentrated in the liquid phase along with free fatty acid (FFA), monoacylglycerol (MG), squalene, tocols, and phytosterols, whereas the solid phase contained mainly triacylglycerol (TG) and steryl esters. Oryzanol-rich product obtained from the first crystallization was subjected to the second crystallization where the oryzanol-rich product was kept at room temperature (20.5+/-1.5 degrees C) for 24h. Hexane was added as anti-solvent to the oryzanol-rich product and kept at 5+/-1 degrees C for another 48h. Parameters that affect the isolation of oryzanol from crude RBO were systematically investigated. Under optimal operation conditions, oryzanol with purity and recovery of 93-95% and 59%, respectively, was obtained from RBO with an initial FFA content of ca. 5%.
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.
authors.forename: Siti
authors.initials: S
authors.lastname: Zullaikah
authors.forename: Elda
authors.initials: E
authors.lastname: Melwita
authors.forename: Yi-Hsu
authors.initials: YH
authors.lastname: Ju
mesh_terms: D003460:Crystallization / Q000379:methods*; D010666:Phenylpropionates / Q000302:isolation & purification*; D010938:Plant Oils / Q000737:chemistry*; D000073879:Rice Bran Oil; D052616:Solid Phase Extraction / Q000379:methods*; D014462:Ultrafiltration / Q000379:methods*
chemical_list: D010666:Phenylpropionates; D010938:Plant Oils; D000073879:Rice Bran Oil; C013172:gamma-oryzanol