title: Genotypic screening of atypical Staphylococcus aureus strains isolated from clinical samples for presence of selected adhesin genes.
abstract: OBJECTIVE
  The aim of this study was to screen Staphylococcus aureus negative for production of coagulase or clumping factor and for presence of selected adhesin genes.
  
  
  METHODS
  Sixty coagulase-negative and 20 clumping factor-negative S. aureus strains were studied. Detection of methicillin resistance was performed using the agar screen technique with 6 mg/L of oxacillin and was confirmed by amplification mecA gene. The presence of bone binding protein (bbp), collagen binding protein (cna), fibronectin A binding protein (fnbA), fibronectin B binding protein (fnbB) and clumping factor A (clfA) genes was detected by multiplex PCR.
  
  
  RESULTS
  Almost all (98%) of the strains were positive for clfA gene. There were fnbA and fnibB in 85%, cna in 54% and bbp in 5% of strains found. No correlation between presence of the particular genes and clinical samples was observed. The prevalence of fnbA, fnbB and cna was statistically higher in coagulase-negative than in clumping factor-negative strains (89, 89, 66 and 70, 70, 15%, respectively). Similarly, all of these genes were more often observed in MRSA than in MSSA atypical strains. The cna was detected only in coagulase-negative MRSA.
authors.affiliation: Department of Medical Microbiology, Medical University of Gdańsk, 38, Do Studzienki Street, 80-227 Gdańsk, Poland. kwis@amg.gda.pl
authors.forename: K
authors.initials: K
authors.lastname: Wiśniewska
authors.forename: K
authors.initials: K
authors.lastname: Garbacz
authors.forename: L
authors.initials: L
authors.lastname: Piechowicz
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics; D015373:Bacterial Typing Techniques / Q000379:methods*; D002352:Carrier Proteins / Q000235:genetics; D003030:Coagulase / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial; D005798:Genes, Bacterial; D006801:Humans; D016106:Methicillin Resistance; D055624:Methicillin-Resistant Staphylococcus aureus / Q000235:genetics / Q000302:isolation & purification; D010068:Oxacillin / Q000494:pharmacology; D011044:Poland / Q000453:epidemiology; D013203:Staphylococcal Infections / Q000453:epidemiology / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000145:classification / Q000187:drug effects / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D018829:Adhesins, Bacterial; D001426:Bacterial Proteins; D002352:Carrier Proteins; D003030:Coagulase; C048165:adhesin, Staphylococcus aureus; C405490:bone sialoprotein-binding protein, Staphylococcus aureus; C078635:fibronectin-binding proteins, bacterial; D010068:Oxacillin