[Effects of asiaticoside on the balance of inflammatory factors of mouse's acute lung injury induced by LPS].
OBJECTIVE
To observe the effect of asiaticoside (AS) on IL-6,TNF-alpha, IL-10 of bronchoaliveolar lavage fluid (BALF) of acute lung injury (ALI) induced by lipopolysaccharides (LPS).


METHODS
56 Balb/c mice were randomly divided into control, model, sham operation,vehicle, model + asiaticoside 5, 15, 45 mg/kg groups. Model of ALI were established by intratracheal instillation of LPS 20 microl ( 2.5 mg/kg), mice were killed 24 hours later, the content of IL-6 ,TNF-alpha, IL-10 of BALF was detected by enzyme-linked immunosorbentassay (ELISA).


RESULTS
AS could reduce the content of IL-6, TNF-alpha and increase IL-10 of BALF in dose-dependent manner.


CONCLUSIONS
The protective effects against ALL induced by LPS on AS are related to reducing the content of IL-6, TNF-alpha, increasing secretion IL-10 and keeping the balance between inflammatory factors and anti-inflammatory factors.
Zhang
Zhuo
Z
Department of Pharmacology, Pharmaceutical Science, Luzhou Medical Colledge, Luzhou 646000, China. zhuozhang100@163.com
Qin
Da-Lian
DL
Wan
Jing-Yuan
JY
Zhou
Qi-Xin
QX
Xiao
Shun-Han
SH
Wu
Ke
K
D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry*; D028041:Centella / Q000737:chemistry*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004797:Enzyme-Linked Immunosorbent Assay; D016753:Interleukin-10 / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D007378:Interleukins / Q000378:metabolism*; D008070:Lipopolysaccharides; D051379:Mice; D008807:Mice, Inbred BALB C; D011897:Random Allocation; D014315:Triterpenes / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
D000893:Anti-Inflammatory Agents; D015850:Interleukin-6; D007378:Interleukins; D008070:Lipopolysaccharides; D014315:Triterpenes; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; C004446:asiaticoside