Nod2 mediates susceptibility to Yersinia pseudotuberculosis in mice.
Nucleotide oligomerisation domain 2 (NOD2) is a component of the innate immunity known to be involved in the homeostasis of Peyer patches (PPs) in mice. However, little is known about its role during gut infection in vivo. Yersinia pseudotuberculosis is an enteropathogen causing gastroenteritis, adenolymphitis and septicaemia which is able to invade its host through PPs. We investigated the role of Nod2 during Y. pseudotuberculosis infection. Death was delayed in Nod2 deleted and Crohn's disease associated Nod2 mutated mice orogastrically inoculated with Y. pseudotuberculosis. In PPs, the local immune response was characterized by a higher KC level and a more intense infiltration by neutrophils and macrophages. The apoptotic and bacterial cell counts were decreased. Finally, Nod2 deleted mice had a lower systemic bacterial dissemination and less damage of the haematopoeitic organs. This resistance phenotype was lost in case of intraperitoneal infection. We concluded that Nod2 contributes to the susceptibility to Y. pseudotuberculosis in mice.
Meinzer
Ulrich
U
INSERM, U843, Université Paris 7, Hôpital Robert Debré, Paris, France.
Esmiol-Welterlin
Sophie
S
Barreau
Frederick
F
Berrebi
Dominique
D
Dussaillant
Monique
M
Bonacorsi
Stephane
S
Chareyre
Fabrice
F
Niwa-Kawakita
Michiko
M
Alberti
Corinne
C
Sterkers
Ghislaine
G
Villard
Claude
C
Lesuffleur
Thecla
T
Peuchmaur
Michel
M
Karin
Michael
M
Eckmann
Lars
L
Giovannini
Marco
M
Ollendorff
Vincent
V
Wolf-Watz
Hans
H
Hugot
Jean-Pierre
JP
D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000378:metabolism; D004198:Disease Susceptibility; D017353:Gene Deletion; D020022:Genetic Predisposition to Disease; D006706:Homeostasis; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic; D053473:Nod2 Signaling Adaptor Protein / Q000235:genetics* / Q000502:physiology*; D010581:Peyer's Patches / Q000382:microbiology*; D010641:Phenotype; D015011:Yersinia pseudotuberculosis / Q000235:genetics*; D015012:Yersinia pseudotuberculosis Infections / Q000235:genetics* / Q000382:microbiology*
D053473:Nod2 Signaling Adaptor Protein; C495945:Nod2 protein, mouse