[Molecular epidemiology of cattle and human tuberculosis in Mexico].
OBJECTIVE
The purpose of the study was to determine the role of bovine TB in cases of human TB.


METHODS
Two-hundred and fifty-five samples from symptomatic patients were included in the study. All samples were cultured in Stonebrink and Lowënstein-Jensen media and analyzed using a nested PCRMPB70. The molecular analysis was performed by spoligotyping.


RESULTS
From 255 samples, 74 were PCR-positive and 20 were culture-positive. From 94 samples positive to PCR or to isolation, 66 (70%) showed a spoligotype compatible with M. tuberculosis, and 13 (13.8%) with M. bovis. Four fingerprints of M. bovis from humans were identical to the fingerprints of M. bovis from cattle in the same region.


CONCLUSIONS
Our study shows that M. bovis plays an important role in the epidemiology of TB in humans and that TB in cattle represents a risk to public health.
Pérez-Guerrero
Laura
L
Programa Nacional de Epidemiología-CENID-FA, INIFAP. Querétaro, México.
Milián-Suazo
Feliciano
F
Arriaga-Díaz
Camila
C
Romero-Torres
Cecilia
C
Escartín-Chávez
Minerva
M
D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D019353:Endemic Diseases; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D017720:Molecular Epidemiology; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology; D014380:Tuberculosis, Bovine / Q000453:epidemiology* / Q000382:microbiology
D004269:DNA, Bacterial