title: Transient increase in the transcript levels of gamma-tubulin complex genes during reorientation of cortical microtubules by gravity in azuki bean (Vigna angularis) epicotyls.
abstract: By hypergravity treatment, the percentage of cells with transverse microtubules was decreased, while that with longitudinal microtubules was increased in azuki bean (Vigna angularis) epicotyls. The expression of genes encoding gamma-tubulin complex (VaTUG and VaGCP3) was increased transiently in response to changes in the gravitational conditions. Lanthanum and gadolinium ions, potential blockers of mechanosensitive calcium ion-permeable channels (mechanoreceptors), nullified reorientation of microtubules as well as up-regulation of expression of VaTUG and VaGCP3 by hypergravity. These results suggest that mechanoreceptors may perceive the gravity signal, which leads to a transient increase in the transcript levels of gamma-tubulin complex genes and reorientation of cortical microtubules.
authors.affiliation: Department of Biology and Geosciences, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka, 558-8585, Japan. soga@sci.osaka-cu.ac.jp.
authors.forename: Kouichi
authors.initials: K
authors.lastname: Soga
authors.forename: Toshihisa
authors.initials: T
authors.lastname: Kotake
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Wakabayashi
authors.forename: Seiichiro
authors.initials: S
authors.lastname: Kamisaka
authors.forename: Takayuki
authors.initials: T
authors.lastname: Hoson
mesh_terms: D002498:Centrifugation; D007887:Fabaceae / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D005682:Gadolinium; D015870:Gene Expression; D018522:Gravitropism; D007811:Lanthanum; D008870:Microtubules / Q000502:physiology*; D014404:Tubulin / Q000235:genetics / Q000378:metabolism*
chemical_list: D014404:Tubulin; D007811:Lanthanum; D005682:Gadolinium