title: Donor screening for hepatitis B virus infection in a cell and tissue bank.
abstract: OBJECTIVE
  Hepatitis B virus (HBV) has been transmitted by tissue transplantation. In order to reduce the risk of HBV transmission, testing for antibody to HBV core antigen (anti-HBc) is used in addition to testing for hepatitis B surface antigen (HBsAg) in many blood centers and tissue banks.
  
  
  METHODS
  We retrospectively analyzed the results of HBV assays in tissue donors. All tissue donors were tested for HBsAg and anti-HBc. All anti-HBc positive sera were tested for the antibody to HBsAg (anti-HBs). From July 2006, an HBV nucleic acid testing (NAT) assay was also performed.
  
  
  RESULTS
  A total of 6855 tissue donors from January 1999 till July 2007 were tested for HBV assays: 4756 women and 2099 men. Positive HBsAg was found in 23 (0.36%) living donors, while no multiorgan or cord blood (CB) donor was found to be positive for HBsAg. Positive anti-HBc was found in 80 multiorgan donors (12.94%), 599 living donors (17.84%), and 103 CB donors (3.57%) (P<0.005), while isolated anti-HBc was found in 12 multiorgan (1.94%), in 126 living tissue donors (3.75%), and in 8 CB donors (0.28%). A total of 1310 donors were analyzed for single-sample DNA HBV NAT assay.
  
  
  CONCLUSIONS
  We consider that anti-HBc and NAT assays must both still be performed in addition to HBsAg assay for HBV screening in tissue donors. All these tests will be useful in order to define an algorithm for safe and efficient management of the tissue bank.
authors.affiliation: Valencia Transfusion Center, Valencia, Spain. solves_pil@gva.es
authors.forename: P
authors.initials: P
authors.lastname: Solves
authors.forename: V
authors.initials: V
authors.lastname: Mirabet
authors.forename: M
authors.initials: M
authors.lastname: Alvarez
authors.forename: E
authors.initials: E
authors.lastname: Vila
authors.forename: F
authors.initials: F
authors.lastname: Quiles
authors.forename: J V
authors.initials: JV
authors.lastname: Villalba
authors.forename: J A
authors.initials: JA
authors.lastname: Montoro
authors.forename: M A
authors.initials: MA
authors.lastname: Soler
authors.forename: R J
authors.initials: RJ
authors.lastname: Roig
mesh_terms: D000293:Adolescent; D000328:Adult; D001782:Blood Donors / Q000600:supply & distribution; D001803:Blood Transfusion; D004279:DNA, Viral / Q000032:analysis* / Q000097:blood; D046148:Donor Selection / Q000379:methods*; D005260:Female; D006509:Hepatitis B / Q000175:diagnosis / Q000517:prevention & control* / Q000635:transmission; D006510:Hepatitis B Antibodies / Q000032:analysis* / Q000097:blood; D006512:Hepatitis B Core Antigens / Q000097:blood; D006514:Hepatitis B Surface Antigens / Q000097:blood; D006515:Hepatitis B virus / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D006801:Humans; D019520:Living Donors / Q000600:supply & distribution; D008297:Male; D008875:Middle Aged; D016377:Organ Transplantation; D012189:Retrospective Studies; D013030:Spain; D014015:Tissue Banks; D014019:Tissue Donors / Q000600:supply & distribution; D055815:Young Adult
chemical_list: D004279:DNA, Viral; D006510:Hepatitis B Antibodies; D006512:Hepatitis B Core Antigens; D006514:Hepatitis B Surface Antigens