title: Mannose-specific interaction of Lactobacillus plantarum with porcine jejunal epithelium.
abstract: Host-microorganism interactions in the intestinal tract are complex, and little is known about specific nonpathogenic microbial factors triggering host responses in the gut. In this study, mannose-specific interactions of Lactobacillus plantarum 299v with jejunal epithelium were investigated using an in situ pig Small Intestinal Segment Perfusion model. The effects of L. plantarum 299v wild-type strain were compared with those of two corresponding mutant strains either lacking the gene encoding for the mannose-specific adhesin (msa) or sortase (srtA; responsible for anchoring of cell surface proteins like Msa to the cell wall). A slight enrichment of the wild-type strain associated with the intestinal surface could be observed after 8 h of perfusion when a mixture of wild-type and msa-mutant strain had been applied. In contrast to the mutant strains, the L. plantarum wild-type strain tended to induce a decrease in jejunal net fluid absorption compared with control conditions. Furthermore, after 8 h of perfusion expression of the host gene encoding pancreatitis-associated protein, a protein with proposed bactericidal properties, was found to be upregulated by the wild-type strain only. These observations suggest a role of Msa in the induction of host responses in the pig intestine.
authors.affiliation: Animal Breeding and Genomics Centre, Animal Sciences Group of Wageningen UR, Lelystad, The Netherlands.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Gross
authors.forename: Jan
authors.initials: J
authors.lastname: van der Meulen
authors.forename: Johannes
authors.initials: J
authors.lastname: Snel
authors.forename: Roelof
authors.initials: R
authors.lastname: van der Meer
authors.forename: Michiel
authors.initials: M
authors.lastname: Kleerebezem
authors.forename: Theo A
authors.initials: TA
authors.lastname: Niewold
authors.forename: Marcel M
authors.initials: MM
authors.lastname: Hulst
authors.forename: Mari A
authors.initials: MA
authors.lastname: Smits
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics / Q000502:physiology; D019881:Aminoacyltransferases / Q000235:genetics / Q000502:physiology; D000818:Animals; D000951:Antigens, Neoplasm / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism; D003546:Cysteine Endopeptidases / Q000235:genetics / Q000502:physiology; D007408:Intestinal Absorption; D007413:Intestinal Mucosa / Q000382:microbiology*; D007583:Jejunum / Q000382:microbiology* / Q000502:physiology; D048191:Lactiplantibacillus plantarum / Q000235:genetics / Q000502:physiology*; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism; D008297:Male; D008358:Mannose / Q000378:metabolism*; D023421:Models, Animal; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D000073718:Pancreatitis-Associated Proteins; D019936:Probiotics; D013552:Swine
chemical_list: D018829:Adhesins, Bacterial; D000951:Antigens, Neoplasm; D001426:Bacterial Proteins; D014408:Biomarkers, Tumor; D037181:Lectins, C-Type; D000073718:Pancreatitis-Associated Proteins; C000612141:REG3A protein, human; C062777:adhesin, Lactobacillus; D019881:Aminoacyltransferases; C120542:sortase A; D003546:Cysteine Endopeptidases; D008358:Mannose