Microcalorimetric study of the effect of Ce(III) on metabolic activity of mitochondria isolated from indice rice 9311.
Rare earth elements (REEs) have beneficial influence on plant growth and are widely used in agriculture practice, but little is known about behavior of the REEs on mitochondria in plant cells. Thermogenic metabolic curves were determined by the ampoule method at 303 K using a TAM air isothermal microcalorimeter in mitochondria isolated from indice rice 9311 (Oryza sativa L.), and the effect of Ce(III) on mitochondrial metabolism was investigated. By analyzing the obtained heat flux curves, the crucial parameters such as activity recovery rate constant (k) and maximum heat power (P(m)) were investigated. Application of Ce3+ in concentrations ranging from 0 to 120 microg/ml significantly increased k and P(m) values, with the maximum reaching 261 and 180% of the control, respectively. Concentrations from 140 to 150 microg/ml had the opposite effect. These results were consistent with previous reports on the effects of REEs on plant growth. It was concluded that the Ce(III)-induced change of mitochondrial metabolic activity is a possible mechanism by which Ce(III) influenced indice rice 9311 growth.
Dai
Jie
J
Department of Chemistry, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei 430072, P. R. China.
Zhang
Ye-Zhong
YZ
Liu
Yi
Y
Li
Qiang-Guo
QG
D002151:Calorimetry; D002563:Cerium / Q000494:pharmacology*; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism* / Q000648:ultrastructure
C032786:cerium nitrate; D002563:Cerium