title: Serological and molecular survey of Rickettsial infection in cattle and sika deer in a pastureland in Hidaka District, Hokkaido, Japan.
abstract: Rickettsial infection of cattle and sika deer from a pastureland in Hidaka District, Hokkaido, Japan was examined by serological and molecular methods. Serum samples from 8 of 83 (9.6%) cattle reacted with Rickettsia helvetica antigens in an IFA test, with titers ranging from 1:160 to 1:640, while serum samples from 15 of 22 (68.2%) deer were positive for R. helvetica, with titers ranging from 1:80 to 1:640. In a genus-specific nested PCR based on gltA, no cattle were positive for Rickettsia, while 14 of 22 (63.3%) samples obtained from deer tested positive. Sequence analysis revealed that positive samples from sika deer showed 100% nucleotide sequence identity with the known sequence of Rickettsia asiatica.
authors.affiliation: Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
authors.lastname: Jilintai
authors.forename: Nobutaka
authors.initials: N
authors.lastname: Seino
authors.forename: Kotaro
authors.initials: K
authors.lastname: Matsumoto
authors.forename: Daisuke
authors.initials: D
authors.lastname: Hayakawa
authors.forename: Masatsugu
authors.initials: M
authors.lastname: Suzuki
authors.forename: Hirosi
authors.initials: H
authors.lastname: Hata
authors.forename: Seiji
authors.initials: S
authors.lastname: Kondo
authors.forename: Naoaki
authors.initials: N
authors.lastname: Yokoyama
authors.forename: Hisashi
authors.initials: H
authors.lastname: Inokuma
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000942:Antigens, Bacterial / Q000097:blood; D002417:Cattle; D002418:Cattle Diseases / Q000453:epidemiology / Q000382:microbiology*; D004269:DNA, Bacterial / Q000032:analysis / Q000302:isolation & purification; D003670:Deer; D007564:Japan / Q000453:epidemiology; D016133:Polymerase Chain Reaction; D012281:Rickettsia / Q000145:classification / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification; D012282:Rickettsia Infections / Q000453:epidemiology / Q000382:microbiology / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D004269:DNA, Bacterial