title: Identification of candidate genes for fenvalerate resistance in Helicoverpa armigera using cDNA-AFLP.
abstract: A cDNA-amplified fragment length polymorphisms approach was undertaken to screen for candidate genes associated with fenvalerate resistance in the AN02 strain of Helicoverpa armigera. Larvae and adults of this strain manifest approximately 50-fold resistance, which is suppressible by piperonyl butoxide and controlled by the semidominant gene RFen1 previously mapped to AFLP Linkage Group 13. Two cytochrome P450s (CYP337B1 and CYP4S1), one carboxylesterase-like protein and one glutathione transferase were found to be constitutively upregulated in resistant insects. Mapping of these potential detoxification genes showed that one of them, the novel P450 CYP337B1, was tightly linked to the resistance locus. This suggests that the RFen1(R) allele has a cis-acting effect on CYP337B1 expression, and possible trans-acting effects on expression of other genes.
authors.affiliation: Centre for Environmental Stress and Adaptation Research, Department of Genetics, The University of Melbourne, Parkville, Victoria 3010, Australia.
authors.forename: C W
authors.initials: CW
authors.lastname: Wee
authors.forename: S F
authors.initials: SF
authors.lastname: Lee
authors.forename: C
authors.initials: C
authors.lastname: Robin
authors.forename: D G
authors.initials: DG
authors.lastname: Heckel
mesh_terms: D000595:Amino Acid Sequence; D054458:Amplified Fragment Length Polymorphism Analysis; D000818:Animals; D018076:DNA, Complementary / Q000235:genetics*; D015536:Down-Regulation; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000502:physiology; D017344:Genes, Insect; D008040:Genetic Linkage; D005819:Genetic Markers; D005838:Genotype; D019476:Insect Proteins / Q000235:genetics / Q000378:metabolism; D007305:Insecticide Resistance / Q000235:genetics*; D007306:Insecticides / Q000494:pharmacology; D008969:Molecular Sequence Data; D009036:Moths / Q000187:drug effects* / Q000235:genetics*; D009570:Nitriles / Q000494:pharmacology*; D011722:Pyrethrins / Q000494:pharmacology*; D015854:Up-Regulation
chemical_list: D018076:DNA, Complementary; D005819:Genetic Markers; D019476:Insect Proteins; D007306:Insecticides; D009570:Nitriles; D011722:Pyrethrins; C017690:fenvalerate