Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool.
We present a platform for the spatially selective sampling of the plasma membrane of single cells. Optically trapped lipid-coated oil droplets (smart droplet microtools, SDMs), typically 0.5-5 microm in size, composed of a hexadecane hydrocarbon core and fusogenic lipid outer coating (mixture of 1,2-dioleoyl-phosphatidylethanolamine and 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine) were brought into controlled contact with target colon cancer cells leading to the formation of connecting membrane tethers. Material transfer from the cell to the SDM across the membrane tether was monitored by tracking membrane-localized enhanced green fluorescent protein.
Lanigan
Peter M P
PM
The Single Cell Proteomics Group, Chemical Biology Centre (CBC), Imperial College London, Exhibition Road, London SW7 2AZ, UK.
Chan
Karen
K
Ninkovic
Tanya
T
Templer
Richard H
RH
French
P M W
PM
de Mello
A J
AJ
Willison
K R
KR
Parker
P J
PJ
Neil
M A A
MA
Ces
Oscar
O
Klug
D R
DR
D002451:Cell Compartmentation; D002458:Cell Fractionation; D002462:Cell Membrane / Q000737:chemistry*; D002469:Cell Separation; D002478:Cells, Cultured; D003584:Cytological Techniques / Q000295:instrumentation; D004655:Emulsions; D006801:Humans; D008055:Lipids; D008561:Membrane Fusion; D008565:Membrane Proteins / Q000032:analysis*; D008856:Microscopy, Fluorescence; D052898:Optical Tweezers; D055095:Optics and Photonics; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*
D004655:Emulsions; D008055:Lipids; D008565:Membrane Proteins