title: [Molecular epidemiology of cattle and human tuberculosis in Mexico].
abstract: OBJECTIVE
  The purpose of the study was to determine the role of bovine TB in cases of human TB.
  
  
  METHODS
  Two-hundred and fifty-five samples from symptomatic patients were included in the study. All samples were cultured in Stonebrink and Lowënstein-Jensen media and analyzed using a nested PCRMPB70. The molecular analysis was performed by spoligotyping.
  
  
  RESULTS
  From 255 samples, 74 were PCR-positive and 20 were culture-positive. From 94 samples positive to PCR or to isolation, 66 (70%) showed a spoligotype compatible with M. tuberculosis, and 13 (13.8%) with M. bovis. Four fingerprints of M. bovis from humans were identical to the fingerprints of M. bovis from cattle in the same region.
  
  
  CONCLUSIONS
  Our study shows that M. bovis plays an important role in the epidemiology of TB in humans and that TB in cattle represents a risk to public health.
authors.affiliation: Programa Nacional de Epidemiología-CENID-FA, INIFAP. Querétaro, México.
authors.forename: Laura
authors.initials: L
authors.lastname: Pérez-Guerrero
authors.forename: Feliciano
authors.initials: F
authors.lastname: Milián-Suazo
authors.forename: Camila
authors.initials: C
authors.lastname: Arriaga-Díaz
authors.forename: Cecilia
authors.initials: C
authors.lastname: Romero-Torres
authors.forename: Minerva
authors.initials: M
authors.lastname: Escartín-Chávez
mesh_terms: D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D019353:Endemic Diseases; D006801:Humans; D008800:Mexico / Q000453:epidemiology; D017720:Molecular Epidemiology; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000235:genetics / Q000302:isolation & purification; D014376:Tuberculosis / Q000453:epidemiology* / Q000382:microbiology; D014380:Tuberculosis, Bovine / Q000453:epidemiology* / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial