title: Fetal ACL fibroblasts exhibit enhanced cellular properties compared with adults.
abstract: Fetal tendons and skin heal regeneratively without scar formation. Cells isolated from these fetal tissues exhibit enhanced cellular migration and collagen production in comparison to cells from adult tissue. We determined whether fetal and adult fibroblasts isolated from the anterior cruciate ligament (ACL), a tissue that does not heal regeneratively, exhibit differences in cell migration rates and collagen elaboration. An in vitro migration assay showed fetal ACL fibroblasts migrated twice as fast as adult ACL fibroblasts at a rate of 38.90 +/- 7.69 microm per hour compared with 18.88 +/- 4.18 microm per hour, respectively. Quantification of Type I collagen elaboration by enzyme-linked immunosorbent assay showed fetal ACL fibroblasts produced four times the amount of Type I collagen compared with adult ACL fibroblasts after 7 days in culture. We observed no differences in Type III collagen with time for adult or fetal ACL fibroblasts. Our findings indicate fetal ACL fibroblasts are intrinsically different from adult ACL fibroblasts, suggesting the healing potential of the ACL may be age-dependent.
authors.affiliation: Department of Bioengineering, University of Pennsylvania, 240 Skirkanich Hall, 210 South 33rd Street, Philadelphia, PA 19104, USA.
authors.forename: Simone S
authors.initials: SS
authors.lastname: Stalling
authors.forename: Steven B
authors.initials: SB
authors.lastname: Nicoll
mesh_terms: D016118:Anterior Cruciate Ligament / Q000166:cytology*; D054443:Cell Migration Assays; D002474:Cell-Free System; D002478:Cells, Cultured; D004797:Enzyme-Linked Immunosorbent Assay; D005333:Fetus / Q000166:cytology*; D005347:Fibroblasts / Q000166:cytology*; D006801:Humans; D014945:Wound Healing / Q000502:physiology*