title: Alternating evolutionary pressure in a genetic algorithm facilitates protein model selection.
abstract: BACKGROUND
  Automatic protein modelling pipelines are becoming ever more accurate; this has come hand in hand with an increasingly complicated interplay between all components involved. Nevertheless, there are still potential improvements to be made in template selection, refinement and protein model selection.
  
  
  RESULTS
  In the context of an automatic modelling pipeline, we analysed each step separately, revealing several non-intuitive trends and explored a new strategy for protein conformation sampling using Genetic Algorithms (GA). We apply the concept of alternating evolutionary pressure (AEP), i.e. intermediate rounds within the GA runs where unrestrained, linear growth of the model populations is allowed.
  
  
  CONCLUSIONS
  This approach improves the overall performance of the GA by allowing models to overcome local energy barriers. AEP enabled the selection of the best models in 40% of all targets; compared to 25% for a normal GA.
authors.affiliation: Biomolecular Modelling Laboratory, Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, London, WC2A 3PX, UK. marc.offman@cancer.org.uk
authors.forename: Marc N
authors.initials: MN
authors.lastname: Offman
authors.forename: Alexander L
authors.initials: AL
authors.lastname: Tournier
authors.forename: Paul A
authors.initials: PA
authors.lastname: Bates
mesh_terms: D000465:Algorithms; D003198:Computer Simulation; D030562:Databases, Protein; D019143:Evolution, Molecular; D008957:Models, Genetic; D008958:Models, Molecular; D011506:Proteins / Q000737:chemistry* / Q000235:genetics*; D011995:Recombination, Genetic; D016415:Sequence Alignment; D012985:Software Design; D013816:Thermodynamics
chemical_list: D011506:Proteins