Expression of glycine-rich protein genes, AtGRP5 and AtGRP23, induced by the cutin monomer 16-hydroxypalmitic acid in Arabidopsis thaliana.
Glycine-rich proteins (GRPs) belong to a large family of heterogenous proteins that are enriched in glycine residues. The expression of two GRP genes of Arabidopsis thaliana, AtGRP5 and AtGRP23, was induced by 16-hydroxypalmitic acid (HPA), a major component of cutin. The expression of AtGRP3, which encodes a GRP protein that is structurally different from AtGRP5 and AtGRP23, was not responsive to HPA application. Treatment with HPA also induced expression of the pathogen-related PR-1 and PR-4 genes. Abscisic acid and salicylic acid treatments enhanced the transcript levels of AtGRP5 and AtGRP23 as well as those of AtGRP3. It was also demonstrated that HPA effectively elicited the accumulation of H2O2 in rosette leaves of Arabidopsis. Results suggest the possible role of some species of GRPs, such as AtGRP5 and AtGRP23, in response to the pathogenic invasion mediated by cutin monomers in plants.
Park
Jong Ho
JH
Department of Biological Sciences, Inha University, Incheon 402-751, Republic of Korea.
Suh
Mi Chung
MC
Kim
Tae Hyun
TH
Kim
Moon Chul
MC
Cho
Sung Ho
SH
D000040:Abscisic Acid / Q000494:pharmacology; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008969:Molecular Sequence Data; D010169:Palmitic Acids / Q000494:pharmacology*; D020156:Salicylic Acid / Q000494:pharmacology; D016415:Sequence Alignment
D029681:Arabidopsis Proteins; D010169:Palmitic Acids; C071359:GRP protein, Arabidopsis; C077262:PR-1 protein, Arabidopsis; C077263:BG2 protein, Arabidopsis; D000040:Abscisic Acid; C063407:16-hydroxypalmitic acid; D020156:Salicylic Acid