title: Reliable enzyme-linked immunosorbent assay for the determination of soybean proteins in processed foods.
abstract: Among allergenic foods, soybean is known as a food causing adverse reactions in allergenic patients. To clarify the validity of labeling, the specific and sensitive detection method for the analysis of the soybean protein would be necessary. The p34 protein, originally characterized to be p34 as an oil-body associated protein in soybean, has been identified as one of the major allergenic proteins and named Gly m Bd 30K. A novel sandwich enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of the soybean protein in processed foods was developed using polyclonal antibodies raised against p34 as a soybean marker protein and the specific extraction buffer for extract. The developed sandwich ELISA method was highly specific for the soybean protein. The limit of detection (LOD) and the limit of quantification (LOQ) of the developed ELISA were 0.47 ng/mL (equivalent to 0.19 microg/g in foods) and 0.94 ng/mL (equivalent to 0.38 microg/g in foods), respectively. The recovery ranged from 87.7 to 98.7%, whereas the intra- and interassay coefficients of variation were less than 4.2 and 7.5%, respectively. This study showed that the developed ELISA method is a specific, precise, and reliable tool for the quantitative analysis of the soybean protein in processed foods.
authors.affiliation: R&D Center, Nippon Meat Packers, Inc., 3-3 Midorigahara, Tsukuba, Ibaraki 300-2646, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Morishita
authors.forename: Kumiko
authors.initials: K
authors.lastname: Kamiya
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsumoto
authors.forename: Shinobu
authors.initials: S
authors.lastname: Sakai
authors.forename: Reiko
authors.initials: R
authors.lastname: Teshima
authors.forename: Atsuo
authors.initials: A
authors.lastname: Urisu
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Moriyama
authors.forename: Tadashi
authors.initials: T
authors.lastname: Ogawa
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Akiyama
authors.forename: Fumiki
authors.initials: F
authors.lastname: Morimatsu
mesh_terms: D000485:Allergens / Q000032:analysis; D052179:Antigens, Plant / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D005511:Food Handling; D005512:Food Hypersensitivity / Q000517:prevention & control; D005515:Food Labeling; D012680:Sensitivity and Specificity; D030262:Soybean Proteins / Q000032:analysis*
chemical_list: D000485:Allergens; D052179:Antigens, Plant; D030262:Soybean Proteins; C075135:P34 protein, Glycine max