Development and validation of an RP-HPLC method to quantitate acyclovir in cross-linked chitosan microspheres produced by spray drying.
An accurate, simple, reproducible, and sensitive liquid chromatographic method is developed and validated to quantitate acyclovir (ACV) in cross-linked chitosan microspheres produced by spray drying. The analysis is carried out using a reversed-phase C18 column with UV-vis detection at 254 nm. The mobile phase is diluted with pure water and acetonitrile (95:5 v/v) at a flow-rate of 0.8 mL/min. The parameters used in the validation process are: linearity, range, quantitation limit, detection limit, accuracy, specificity precision, and ruggedness. The retention time of acyclovir is approximately 3.5 min with symmetrical peaks. The linearity in the range of 1-10 microg/mL presents a correlation coefficient of 0.9999. The chitosan and the tripolyphosphate in the formulation do not interfere with the analysis, and the recovery is quantitative. Results are satisfactory, and the method proves to be suitable to quantitate ACV in cross-linked chitosan microspheres.
Stulzer
Hellen K
HK
Laboratório Quitech, Departamento de Química, Universidade Federal de Santa Catarina. hellen.stulzer@gmail.com
Tagliari
Monika Piazzon
MP
Murakami
Fábio S
FS
Silva
Marcos A S
MA
Laranjeira
Mauro C M
MC
D000212:Acyclovir / Q000032:analysis*; D000998:Antiviral Agents / Q000032:analysis*; D048271:Chitosan / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D008863:Microspheres; D012015:Reference Standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
D000998:Antiviral Agents; D048271:Chitosan; D000212:Acyclovir