title: JMV-1 stimulation of avian natural killer cell activity.
abstract: The phenotypic characterization of the non-productive, Marek's disease virus-transformed, lymphoblastoid cell line, JMV-1 and the immunological effects of its cell-free culture supernatant were examined. It was verified that the JMV-1 cell line is an activated T-helper cell line that bears antigens indicative of T-cell activation, and it is not a natural killer or cytotoxic T-cell line. Furthermore, the JMV-1 cell line was shown to be unique among Marek's disease-transformed cell lines in that JMV-1 cells secrete factors that are able to stimulate in vitro natural killer cell cytotoxicity against the avian tumour cell line, LSCC-RP9, in a 4-h assay. Culturing spleen cells in the presence of JMV-1 supernatant for 4 h stimulates a significant increase in NK cell populations and in la-bearing cell populations, as detected by monoclonal antibody staining. These findings suggest that the protective effects against the parasitic and virally-induced lymphoproliferative poultry diseases, which have been credited to the JMV-1 cell line and to its cell-free culture supernatant, are in part due to lymphokine activation of NK cell cytotoxicity.
authors.affiliation: School of Veterinary Medicine, University of Pennsylvania, Beltsville, Maryland, USA.
authors.forename: L H
authors.initials: LH
authors.lastname: Keller
authors.forename: H S
authors.initials: HS
authors.lastname: Lillehoj
authors.forename: J M
authors.initials: JM
authors.lastname: Solnosky