title: vacA genotypes of Helicobacter pylori in relation to cagA status and clinical outcomes in Iranian populations.
abstract: Mosaicism in vacA alleles with two distinct families of vacA signal sequences (s1 and s2) and two distinct families of middle region alleles (m1 and m2) has been reported. Research suggests that the vacA s1 genotype is closely associated with duodenal ulcer disease and with high cytotoxin production. The aims of this study were to evaluate the role of vacA genotyping with respect to gastric inflammation and injury, and clinical presentation in Iranian populations. Genomic DNA of biopsy specimens from patients with gastritis, peptic ulcer disease (PUD), or gastric cancer (GC) were characterized based on ureC (glmM), cagA, and vacA genotyping by using polymerase chain reaction. Of 167 patients including 33 with PUDs, 129 with non-ulcer dyspepsia (NUD), and 5 with GC, 96 (57.5%) cases were infected by Helicobacter pylori. Among these patients, H. pylori were isolated from 19 (57.7%) PUD patients, 74 (68.7%) NUD patients, and 3 (60%) GC patients. The cagA was detected in 76% of H. pylori-positive cases. The vacA s1-m2 genotype was the most prevalent in 7/19 PUD (37%) and 30/74 NUD (40.5%) patients with H. pylori infection. The prevalence of vacA s2-m1 (8%) was high in Iranian isolates. A significant association was not found between H. pylori genotypes and clinical outcomes. The vacA genotypes and cagA status were not useful markers for gastroduodenal diseases in Tehran, Iran.
authors.affiliation: Research Center for Gastroenterology and Liver Diseases in Shaheed Beheshti University, M. C., Tehran, Iran. f_jaafari580@yahoo.com.
authors.forename: Fereshteh
authors.initials: F
authors.lastname: Jafari
authors.forename: Lleila
authors.initials: L
authors.lastname: Shokrzadeh
authors.forename: Hossein
authors.initials: H
authors.lastname: Dabiri
authors.forename: Kaveh
authors.initials: K
authors.lastname: Baghaei
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamaoka
authors.forename: Homayon
authors.initials: H
authors.lastname: Zojaji
authors.forename: Mehrdad
authors.initials: M
authors.lastname: Haghazali
authors.forename: Masha
authors.initials: M
authors.lastname: Molaei
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Zali
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000942:Antigens, Bacterial / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002648:Child; D005260:Female; D005756:Gastritis / Q000453:epidemiology / Q000382:microbiology / Q000503:physiopathology; D005838:Genotype; D016481:Helicobacter Infections / Q000453:epidemiology* / Q000382:microbiology / Q000503:physiopathology*; D016480:Helicobacter pylori / Q000235:genetics / Q000472:pathogenicity*; D006801:Humans; D007492:Iran / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D010437:Peptic Ulcer / Q000453:epidemiology / Q000382:microbiology / Q000503:physiopathology; D015995:Prevalence; D013274:Stomach Neoplasms / Q000453:epidemiology / Q000382:microbiology / Q000503:physiopathology
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; C074689:VacA protein, Helicobacter pylori; C080866:cagA protein, Helicobacter pylori