Identification of very-long-chain polyunsaturated fatty acids from Amphidinium carterae by atmospheric pressure chemical ionization liquid chromatography-mass spectroscopy.
A method is described for the enrichment of very-long-chain polyunsaturated fatty acids (VLCPUFAs) from total fatty acids of Amphidinium carterae and their identification as picolinyl esters by means of microbore liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS/APCI). The combination of argentation TLC and LC-MS/APCI was used to identify unusual VLCPUFAs up to hexatriacontaoctaenoic acid. Two acids, 36:7n-6 and 36:8n-3, were also synthesized to unambiguously confirm their structure. The possibilities of VLCPUFAs biosynthesis are proposed.
Rezanka
Tomás
T
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, 142 20 Prague, Czech Republic. rezanka@biomed.cas.cz
Nedbalová
Linda
L
Sigler
Karel
K
D000818:Animals; D004141:Dinoflagellida / Q000737:chemistry*; D004777:Environment; D005231:Fatty Acids, Unsaturated / Q000032:analysis* / Q000737:chemistry; D013058:Mass Spectrometry; D008958:Models, Molecular; D012623:Seawater; D013055:Spectrophotometry, Infrared; D013056:Spectrophotometry, Ultraviolet
D005231:Fatty Acids, Unsaturated