title: High-risk human papillomavirus infections in breast cancer in Syrian women and their association with Id-1 expression: a tissue microarray study.
abstract: High-risk human papillomaviruses (HPVs) could be important risk factors for breast carcinogenesis and metastasis. Based on this hypothesis, we recently studied the effect of E6/E7 onco-proteins of high-risk HPV type 16 in two non-invasive human breast cancer cell lines, BT20 and MCF7; we reported that E6/E7 converts these cell lines to invasive cells. This is accompanied by an overexpression of Id-1, which is an important regulator of breast metastasis. In this investigation, we examined the presence of high-risk HPVs (16, 18, 31, 33 and 35) and the expression of their E6 onco-protein as well as their correlation with Id-1 gene expression, using polymerase chain reaction (PCR) and tissue microarray (TMA) analysis, respectively, in a cohort of 113 Syrian breast cancer patients. We found that high-risk HPV types 16, 18, 31, 33 and 35 are present in 8.84, 9.73, 7.07, 55.75 and 37.16% of our samples, respectively, which represent invasive breast cancers. Overall, 69 (61.06%) of the 113 samples are HPV positive; among these specimens 24 tissues (34.78%) are coinfected with more than one HPV type. Furthermore, we report that the expression of the E6 onco-protein of these high-risk HPVs is correlated with Id-1 overexpression in the majority of invasive breast cancer tissue samples. Our data suggest that high-risk HPV infections are associated with human breast cancer progression in Syrian women.
authors.affiliation: Faculty of Medicine, University of Aleppo, Aleppo, Syria.
authors.forename: N
authors.initials: N
authors.lastname: Akil
authors.forename: A
authors.initials: A
authors.lastname: Yasmeen
authors.forename: A
authors.initials: A
authors.lastname: Kassab
authors.forename: L
authors.initials: L
authors.lastname: Ghabreau
authors.forename: A D
authors.initials: AD
authors.lastname: Darnel
authors.forename: A-E
authors.initials: AE
authors.lastname: Al Moustafa
mesh_terms: D000328:Adult; D000368:Aged; D052685:Alphapapillomavirus / Q000235:genetics / Q000302:isolation & purification*; D001483:Base Sequence; D001943:Breast Neoplasms / Q000473:pathology* / Q000821:virology*; D045744:Cell Line, Tumor; D015331:Cohort Studies; D017931:DNA Primers; D005260:Female; D005814:Genes, Viral; D006801:Humans; D007150:Immunohistochemistry; D051798:Inhibitor of Differentiation Protein 1 / Q000096:biosynthesis*; D008875:Middle Aged; D009362:Neoplasm Metastasis; D009856:Oncogene Proteins, Viral / Q000235:genetics; D030361:Papillomavirus Infections / Q000150:complications*; D016133:Polymerase Chain Reaction; D013593:Syria / Q000453:epidemiology; D046888:Tissue Array Analysis
chemical_list: D017931:DNA Primers; C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; D009856:Oncogene Proteins, Viral