title: A uniaxial bioMEMS device for imaging single cell response during quantitative force-displacement measurements.
abstract: A microfabricated device has been developed for imaging of a single, adherent cell while quantifying force under an applied displacement. The device works in a fashion similar to that of a displacement-controlled uniaxial tensile machine. The device was calibrated using a tipless atomic force microscope (AFM) cantilever and shows excellent agreement with the calculated spring constant. A step input was applied to a single, adherent fibroblast cell and the viscoelastic response was characterized with a mechanical model. The adherent fibroblast was imaged by use of epifluorescence and phase contrast techniques.
authors.affiliation: National Institute of Standards and Technology, Boulder, CO, USA. david.serrell@colorado.edu.|University of Colorado, Boulder, CO, USA. david.serrell@colorado.edu.
authors.forename: David B
authors.initials: DB
authors.lastname: Serrell
authors.affiliation: National Institute of Standards and Technology, Boulder, CO, USA.
authors.forename: Jera
authors.initials: J
authors.lastname: Law
authors.affiliation: National Institute of Standards and Technology, Boulder, CO, USA.
authors.forename: Andrew J
authors.initials: AJ
authors.lastname: Slifka
authors.affiliation: Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
authors.forename: Roop L
authors.initials: RL
authors.lastname: Mahajan
authors.affiliation: Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.
authors.forename: Dudley S
authors.initials: DS
authors.lastname: Finch
mesh_terms: D000818:Animals; D002460:Cell Line; D006224:Cricetinae; D005347:Fibroblasts / Q000648:ultrastructure*; D046210:Microfluidic Analytical Techniques; D018625:Microscopy, Atomic Force / Q000295:instrumentation* / Q000379:methods