Expression of various types of alternatively spliced WAPL transcripts in human cervical epithelia.
We have previously identified a novel gene WAPL that is inducible by human papillomavirus (HPV) E6 and E7 oncoproteins, and is associated with uterine cervical carcinogenesis. A WAPL splice variant named Friend of EBNA2 (FOE) has also been characterized as a binding partner of the Epstein-Barr virus transformation-related protein EBNA2. On the other hand, recent studies have revealed that WAPL is a cohesin-binding protein and promotes sister-chromatid resolution in mitotic prophase. These data demonstrate that WAPL plays an important role in tumorigenesis and cell cycle progression. In this study, we have isolated a large number of additional alternatively spliced WAPL variants from various cervical epithelia. Each variant consists of a variable 5'-terminal region and the conserved remainder. In addition, we have confirmed the genomic organization of the 5'-region of the WAPL gene, and have investigated the characteristic features of the WAPL variants and their products. Furthermore, we have determined the HPV types of the expressed E6/E7 transcripts in the cervical epithelia with a novel PCR protocol. These results should shed light on a novel aspect of WAPL function.
Oikawa
Kosuke
K
Department of Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
Akiyoshi
Ayumi
A
Tanaka
Masami
M
Takanashi
Masakatsu
M
Nishi
Hirotaka
H
Isaka
Keiichi
K
Kiseki
Hisami
H
Idei
Tomoko
T
Tsukahara
Yutaka
Y
Hashimura
Naohiko
N
Mukai
Kiyoshi
K
Kuroda
Masahiko
M
D017398:Alternative Splicing; D000595:Amino Acid Sequence; D001483:Base Sequence; D002352:Carrier Proteins / Q000235:genetics*; D002460:Cell Line; D002584:Cervix Uteri / Q000378:metabolism* / Q000821:virology; D018076:DNA, Complementary / Q000235:genetics; D004848:Epithelium / Q000378:metabolism / Q000821:virology; D005260:Female; D015870:Gene Expression; D006801:Humans; D008969:Molecular Sequence Data; D009687:Nuclear Proteins / Q000235:genetics*; D027383:Papillomaviridae / Q000235:genetics / Q000472:pathogenicity; D011518:Proto-Oncogene Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics; D012367:RNA, Viral / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D002583:Uterine Cervical Neoplasms / Q000209:etiology / Q000235:genetics / Q000378:metabolism / Q000821:virology; D000097722:Cohesins
D002352:Carrier Proteins; D018076:DNA, Complementary; D009687:Nuclear Proteins; D011518:Proto-Oncogene Proteins; D012333:RNA, Messenger; D012367:RNA, Viral; C488092:WAPL protein, human; D000097722:Cohesins