title: Soluble amyloid precursor proteins in the cerebrospinal fluid as novel potential biomarkers of Alzheimer's disease: a multicenter study.
abstract: In this report, we present the results of a multicenter study to test analytic and diagnostic performance of soluble forms of amyloid precursor proteins alpha and beta (sAPP alpha and sAPP beta) in the cerebrospinal fluid (CSF) of patients with different forms of dementing conditions. CSF samples were collected from 188 patients with early dementia (mini-mental state examination >or=20 in majority of cases) and mild cognitive impairment (MCI) in 12 gerontopsychiatric centers, and the clinical diagnoses were supported by neurochemical dementia diagnostic (NDD) tools: CSF amyloid beta peptides, Tau and phospho-Tau. sAPP alpha and sAPP beta were measured with multiplexing method based on electrochemiluminescence. sAPP alpha and sAPP beta CSF concentrations correlated with each other with very high correlation ratio (R=0.96, P<0.001). We observed highly significantly increased sAPP alpha and sAPP beta CSF concentrations in patients with NDD characteristic for Alzheimer's disease (AD) compared to those with NDD negative results. sAPP alpha and sAPP beta highly significantly separated patients with AD, whose diagnosis was supported by NDD findings (sAPP alpha: cutoff, 117.4 ng ml(-1), sensitivity, 68%, specificity, 85%, P<0.001; sAPP beta: cutoff, 181.8 ng ml(-1), sensitivity, 75%, specificity, 85%, P<0.001), from the patients clinically assessed as having other dementias and supported by NDD untypical for AD. We conclude sAPP alpha and sAPP beta might be regarded as novel promising biomarkers supporting the clinical diagnosis of AD.
authors.affiliation: Department of Psychiatry and Psychotherapy, University of Erlangen-Nuremberg, Erlangen, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Lewczuk
authors.forename: H
authors.initials: H
authors.lastname: Kamrowski-Kruck
authors.forename: O
authors.initials: O
authors.lastname: Peters
authors.forename: I
authors.initials: I
authors.lastname: Heuser
authors.forename: F
authors.initials: F
authors.lastname: Jessen
authors.forename: J
authors.initials: J
authors.lastname: Popp
authors.forename: K
authors.initials: K
authors.lastname: Bürger
authors.forename: H
authors.initials: H
authors.lastname: Hampel
authors.forename: L
authors.initials: L
authors.lastname: Frölich
authors.forename: S
authors.initials: S
authors.lastname: Wolf
authors.forename: B
authors.initials: B
authors.lastname: Prinz
authors.forename: H
authors.initials: H
authors.lastname: Jahn
authors.forename: Ch
authors.initials: Ch
authors.lastname: Luckhaus
authors.forename: R
authors.initials: R
authors.lastname: Perneczky
authors.forename: M
authors.initials: M
authors.lastname: Hüll
authors.forename: J
authors.initials: J
authors.lastname: Schröder
authors.forename: H
authors.initials: H
authors.lastname: Kessler
authors.forename: J
authors.initials: J
authors.lastname: Pantel
authors.forename: H-J
authors.initials: HJ
authors.lastname: Gertz
authors.forename: H-W
authors.initials: HW
authors.lastname: Klafki
authors.forename: H
authors.initials: H
authors.lastname: Kölsch
authors.forename: U
authors.initials: U
authors.lastname: Reulbach
authors.forename: H
authors.initials: H
authors.lastname: Esselmann
authors.forename: J M
authors.initials: JM
authors.lastname: Maler
authors.forename: M
authors.initials: M
authors.lastname: Bibl
authors.forename: J
authors.initials: J
authors.lastname: Kornhuber
authors.forename: J
authors.initials: J
authors.lastname: Wiltfang
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000134:cerebrospinal fluid*; D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid; D016564:Amyloid beta-Protein Precursor / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D003072:Cognition Disorders / Q000134:cerebrospinal fluid; D003704:Dementia / Q000134:cerebrospinal fluid; D005260:Female; D005858:Germany; D006801:Humans; D008297:Male; D008875:Middle Aged; D011569:Psychiatric Status Rating Scales; D012680:Sensitivity and Specificity; D013223:Statistics as Topic; D016875:tau Proteins / Q000134:cerebrospinal fluid
chemical_list: D016229:Amyloid beta-Peptides; D016564:Amyloid beta-Protein Precursor; D015415:Biomarkers; D016875:tau Proteins