Comparative analysis of transgenic tall fescue (Festuca arundinacea Schreb.) plants obtained by Agrobacterium-mediated transformation and particle bombardment.
Agrobacterium-mediated transformation and particle bombardment are the two most widely used methods for genetically modifying grasses. Here, these two systems are compared for transformation efficiency, transgene integration and transgene expression when used to transform tall fescue (Festuca arundinacea Schreb.). The bar gene was used as a selectable marker and selection during tissue culture was performed using 2 mg/l bialaphos in both callus induction and regeneration media. Average transformation efficiency across the four callus lines used in the experiments was 10.5% for Agrobacterium-mediated transformation and 11.5% for particle bombardment. Similar transgene integration patterns and co-integration frequencies of bar and uidA were observed in both gene transfer systems. However, while GUS activity was detected in leaves of 53% of the Agrobacterium transformed lines, only 20% of the bombarded lines showed GUS activity. Thus, Agrobacterium-mediated transformation appears to be the preferred method for producing transgenic tall fescue plants.
Gao
Caixia
C
Research Division, DLF-Trifolium Ltd, Hoejerupvej 31, 4660, Store Heddinge, Denmark. cxg@dlf.dk
Long
Danfeng
D
Lenk
Ingo
I
Nielsen
Klaus Kristian
KK
D019470:Biolistics; D015139:Blotting, Southern; D018744:DNA, Plant / Q000378:metabolism; D031751:Festuca / Q000235:genetics* / Q000382:microbiology*; D018628:Gene Dosage; D018506:Gene Expression Regulation, Plant; D005822:Genetic Vectors / Q000235:genetics; D030821:Plants, Genetically Modified; D012231:Rhizobium / Q000378:metabolism*; D012641:Selection, Genetic; D046509:Tissue Culture Techniques; D014170:Transformation, Genetic; D019076:Transgenes
D018744:DNA, Plant