title: Distribution and application of ELISA for the seroprevalence of tick-borne diseases in Central Equatoria State, Sudan.
abstract: This study was carried out to estimate the prevalence of Theileria parva, Theileria mutans, Babesia bigemina and Anaplasma marginale antibodies among naturally infected cattle in Central Equatoria State, Southern Sudan using indirect enzyme-linked immunosorbent assay (ELISA). Three locations (Khor Rumla, Nyaing and Gumbo) were selected and surveyed every 3 months during the period from January to December 2005. Six cattle herds in the three locations were visited four times during the study period. During each visit, 150 serum samples were collected from indigenous cattle of both sex and of different age groups. Assessment of antibodies against tick-borne diseases was carried out using indirect ELISA. The overall seroprevalence rates of T. parva, T. mutans, B. bigemina and A. marginale were found to be 58.2%, 88.9%, 51.1% and 37.8% respectively. The highest seroprevalence of T. parva was observed in Gumbo (69%). An overall seroprevalence of 43.3% for T. parva was reported in July which was significantly lower than other seasons. Older animals showed higher seroprevalence than younger ones. The implications of these results on the epidemiology of tick-borne diseases are discussed with emphasis on East Coast fever.
authors.affiliation: Central Veterinary Research Laboratories, Al Amarat, PO Box 8067, Khartoum, Sudan. diaeldin2000@hotmail.com
authors.forename: D A
authors.initials: DA
authors.lastname: Salih
authors.forename: S M
authors.initials: SM
authors.lastname: Hassan
authors.forename: I I
authors.initials: II
authors.lastname: Julla
authors.forename: M N
authors.initials: MN
authors.lastname: Kyule
authors.forename: K-H
authors.initials: KH
authors.lastname: Zessin
authors.forename: A M
authors.initials: AM
authors.lastname: El Hussein
mesh_terms: D042322:Anaplasma marginale / Q000276:immunology; D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D000913:Antibodies, Protozoan / Q000097:blood*; D001403:Babesia / Q000276:immunology; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000453:epidemiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary*; D012621:Seasons; D016036:Seroepidemiologic Studies; D013397:Sudan / Q000453:epidemiology; D016794:Theileria / Q000276:immunology; D016797:Theileria parva / Q000276:immunology; D017282:Tick-Borne Diseases / Q000097:blood / Q000453:epidemiology / Q000662:veterinary*
chemical_list: D000907:Antibodies, Bacterial; D000913:Antibodies, Protozoan