tmRNA-dependent trans-translation is required for sporulation in Bacillus subtilis.
Spore formation in Bacillus subtilis is significantly impaired by the deletion of the gene for tmRNA (ssrA), which facilitates the trans-translation reaction that rescues stalled ribosomes and degrades incompletely synthesized peptides. Microscopic analysis revealed that the sporulation of most DeltassrA cells is blocked after forespore formation. Expression analysis of lacZ-fused genes directed by several RNA polymerase sigma factors showed that the synthesis of active sigma(K), encoded by the sigK gene, is predominantly inhibited in DeltassrA cells. The defect in sigma(K) synthesis is attributable to a defect in the skin element excision, which generates the sigK gene, caused in turn by reduced expression of SpoIVCA (recombinase) in DeltassrA cells.
Abe
Teppei
T
Department of Biochemistry and Molecular Biology, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki 036-8561, Japan.
Sakaki
Kazutaka
K
Fujihara
Ai
A
Ujiie
Hiromi
H
Ushida
Chisato
C
Himeno
Hyouta
H
Sato
Tsutomu
T
Muto
Akira
A
D001412:Bacillus subtilis / Q000166:cytology / Q000502:physiology*; D001426:Bacterial Proteins / Q000096:biosynthesis; D017353:Gene Deletion; D017930:Genes, Reporter; D008565:Membrane Proteins / Q000096:biosynthesis; D014176:Protein Biosynthesis; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism*; D013171:Spores, Bacterial / Q000166:cytology / Q000254:growth & development*; D014157:Transcription Factors / Q000096:biosynthesis; D001616:beta-Galactosidase / Q000235:genetics / Q000378:metabolism
D001426:Bacterial Proteins; D008565:Membrane Proteins; D012329:RNA, Bacterial; D014157:Transcription Factors; C084190:sigma K; C088067:spoIV protein, Bacillus megaterium; C096222:tmRNA; D001616:beta-Galactosidase