title: A conventional LC-MS method developed for the determination of plasma raltegravir concentrations.
abstract: Raltegravir belongs to a new class of antiretrovirals acting for a human immunodeficiency virus (HIV)-1 integrase inhibition. Clinical trials of this drug have demonstrated potent antiviral activity in both therapy naïve and experienced patients. Thus, raltegravir has become an important component of combination treatment regimens used to treat patients with multidrug-resistant HIV-1. The quantification of raltegravir in human plasma is important to support clinical studies and determine pharmacokinetic parameters of raltegravir in HIV-1 infected patients. Recently, the LC-MS/MS superfine system was developed to determine plasma concentration of raltegravir; however, the system needs to be delicately set and the equipment is very expensive. Therefore, we developed a conventional LC-MS method to overcome these difficulties. Subsequently the method was validated by estimating the precision and accuracy for inter- and intraday analysis in the concentration range of 0.010-7.680 microg/ml. The calibration curve was linear in this range. Average accuracy ranged from 97.2 to 103.4%. Relative standard deviations of both inter- and intraday assays were less than 10.4%. Recovery of raltegravir was more than 80.6%. This novel LC-MS method is accurate and precise enough to determine raltegravir levels in human plasma samples.
authors.affiliation: Department of Pharmacy, National Hospital Organization Nagoya Medical Center (Tokai Area Central Hospital for AIDS Treatment and Research), Nagoya, Aichi, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Takahashi
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Konishi
authors.forename: Yuichi
authors.initials: Y
authors.lastname: Kudaka
authors.forename: Naoya
authors.initials: N
authors.lastname: Okumura
authors.forename: Atsushi
authors.initials: A
authors.lastname: Hirano
authors.forename: Nami
authors.initials: N
authors.lastname: Terahata
authors.forename: Kazuhide
authors.initials: K
authors.lastname: Banno
authors.forename: Tsuguhiro
authors.initials: T
authors.lastname: Kaneda
mesh_terms: D000284:Administration, Oral; D002853:Chromatography, Liquid; D015658:HIV Infections / Q000097:blood; D019428:HIV Integrase Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D006801:Humans; D007202:Indicators and Reagents; D013058:Mass Spectrometry; D011760:Pyrrolidinones / Q000097:blood* / Q000493:pharmacokinetics; D000068898:Raltegravir Potassium; D015203:Reproducibility of Results
chemical_list: D019428:HIV Integrase Inhibitors; D007202:Indicators and Reagents; D011760:Pyrrolidinones; D000068898:Raltegravir Potassium