title: Selective and potent in vitro antitrypanosomal activities of ten microbial metabolites.
abstract: More than 400 compounds isolated from soil microorganisms, and catalogued in the antibiotic library of the Kitasato Institute for Life Sciences, were screened against African trypanosomes. Ten compounds were found to have selective and potent antitrypanosomal activity in vitro: aureothin, cellocidin, destomycin A, echinomycin, hedamycin, irumamycin, LL-Z 1272beta, O-methylnanaomycin A, venturicidin A and virustomycin A. Results of the in vitro assays using the GUTat 3.1 strain of Trypanosomal brucei brucei and the STIB900 strain of T. b. rhodesiense are presented. Cytotoxicity was determined using a human MRC-5 cell line. This is the first report of antitrypanosomal activities of the 10 microbial metabolites listed above.
authors.affiliation: Research Center for Tropical Diseases, The Kitasato Institute, Tokyo, Japan. otoguro@lisci.kitasato-u.ac.jp
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Otoguro
authors.forename: Aki
authors.initials: A
authors.lastname: Ishiyama
authors.forename: Miyuki
authors.initials: M
authors.lastname: Namatame
authors.forename: Aki
authors.initials: A
authors.lastname: Nishihara
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Furusawa
authors.forename: Rokuro
authors.initials: R
authors.lastname: Masuma
authors.forename: Kazuro
authors.initials: K
authors.lastname: Shiomi
authors.forename: Yoko
authors.initials: Y
authors.lastname: Takahashi
authors.forename: Haruki
authors.initials: H
authors.lastname: Yamada
authors.forename: Satoshi
authors.initials: S
authors.lastname: Omura
mesh_terms: D000480:Alkynes / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000818:Animals; D000880:Anthraquinones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D002460:Cell Line; D002867:Chromones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D004448:Echinomycin / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D006921:Hygromycin B / Q000031:analogs & derivatives / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D018942:Macrolides / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D009285:Naphthoquinones / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D012988:Soil Microbiology; D014344:Trypanocidal Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects*; D014698:Venturicidins / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D000095622:Benzoisochromanequinones
chemical_list: D000480:Alkynes; D000880:Anthraquinones; D002867:Chromones; D004448:Echinomycin; D006921:Hygromycin B; D018942:Macrolides; D009285:Naphthoquinones; D014344:Trypanocidal Agents; D014698:Venturicidins; C001328:hedamycin; C015739:cellocidin; C068161:venturicidin A; C008638:nanaomycin A; D000095622:Benzoisochromanequinones; C034626:irumamycin; C037255:virustomycin A; C012198:aureothin; C026091:destomycin A