title: NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B.
abstract: The NMR structure of budding yeast chaperone Chz1 complexed with histones H2A.Z-H2B has been determined. Chz1 forms a long irregular chain capped by two short alpha-helices, and uses both positively and negatively charged residues to stabilize the histone dimer. A molecular model that docks Chz1 onto the nucleosome has implications for its potential functions.
authors.affiliation: Laboratory of Biochemistry and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.forename: Hanqiao
authors.initials: H
authors.lastname: Feng
authors.forename: D Flemming
authors.initials: DF
authors.lastname: Hansen
authors.forename: Hidenori
authors.initials: H
authors.lastname: Kato
authors.forename: Ed
authors.initials: E
authors.lastname: Luk
authors.forename: Daron I
authors.initials: DI
authors.lastname: Freedberg
authors.forename: Lewis E
authors.initials: LE
authors.lastname: Kay
authors.forename: Carl
authors.initials: C
authors.lastname: Wu
authors.forename: Yawen
authors.initials: Y
authors.lastname: Bai
mesh_terms: D001120:Arginine / Q000737:chemistry; D002244:Carbon / Q000737:chemistry; D019281:Dimerization; D056488:Histone Chaperones; D006657:Histones / Q000737:chemistry*; D008239:Lysine / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D018832:Molecular Chaperones / Q000737:chemistry*; D008968:Molecular Conformation; D009584:Nitrogen / Q000737:chemistry; D009707:Nucleosomes / Q000737:chemistry; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D012441:Saccharomyces cerevisiae / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000737:chemistry*; D055672:Static Electricity
chemical_list: C518297:Chz1 protein, S cerevisiae; D056488:Histone Chaperones; D006657:Histones; C493530:Htz1 protein, S cerevisiae; D018832:Molecular Chaperones; D009707:Nucleosomes; D029701:Saccharomyces cerevisiae Proteins; D002244:Carbon; D001120:Arginine; D008239:Lysine; D009584:Nitrogen