[Octreotide induces apoptosis of human hepatoma cells by the mechanism of facilitating the Fas/FasL gene expression therein].
OBJECTIVE
To investigate the effects of octreotide on apoptosis of and Fas/FasL gene expression in human hepatoma cell mechanism of the suppression effect of liver cancer with e in order to offer the experimental foundation for clinic treatment.


METHODS
Hyman hematoma cells of the line SMMC-7721 were cultured and divided into two groups: octreotide group, co-cultured with octreotide 4,7, and 10 microg/ml respectively for 24 h, 48 h, or 72 h, and control group. TUNEL was used to detect the apoptosis of the cells. Flow cytometry was used with terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) technique to detect the apoptosis of the cells. FC was used with direct labeling of monoclonal antibody (CD95/CD178) to detect the expression of Fas and FasL, and the Fas/FasL ratio.


RESULTS
Octreotide increased the apoptotic rate of the cells dose- and time-dependently (all P < 0.05). Octreotide increased the Fas expression rate, FasL expression rate, and Fas/FasL ratio dose- and time-dependently (all P < 0.05).


CONCLUSIONS
Octreotide induces the apoptosis of human hepatoma cells, possibly by the mechanism of facilitating the Fas/FasL gene expression.
Ma
Qi
Q
Department of General Surgery, Central Hospital of CNPC, Langfang 065000, China. maqizy@yahoo.com.cn
Meng
Ling-Quan
LQ
Liu
Jing-Chang
JC
Hu
Jian-Ping
JP
Ge
Jun
J
Wan
Yu-Liang
YL
Jiang
Shan
S
D018931:Antineoplastic Agents, Hormonal / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D053222:Fas Ligand Protein / Q000096:biosynthesis*; D005434:Flow Cytometry; D006801:Humans; D020287:In Situ Nick-End Labeling; D008113:Liver Neoplasms / Q000378:metabolism / Q000473:pathology; D015282:Octreotide / Q000494:pharmacology*; D013997:Time Factors; D019014:fas Receptor / Q000096:biosynthesis*
D018931:Antineoplastic Agents, Hormonal; D053222:Fas Ligand Protein; D019014:fas Receptor; D015282:Octreotide