Psoroptes ovis: identification of vaccine candidates by immunoscreening.
Serum from successful vaccine trials against the sheep scab mite, Psoroptes ovis, was used to immunoscreen a cDNA library constructed from mixed-stage and gender P. ovis to identify potential recombinant vaccine candidates. Immunodominant recombinant proteins recognised by IgG in these sera were selected for further analysis. Two candidates were identified in this way; a catchin-like protein (CLP) and a novel mu class glutathione S-transferase (GST). Both candidates were expressed in bacteria as recombinant proteins, the GST as an active enzyme, and combined with four other recombinant allergens in a multi-component recombinant vaccine. Strong serum IgG responses were induced in sheep against each of the components of the recombinant vaccine, however, the protective efficacy of the vaccine could not be determined because of variability in the establishment of a challenge infection.
Nisbet
Alasdair J
AJ
Moredun Research Institute, Parasitology Division, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, Scotland, UK. Alasdair.Nisbet@moredun.ac.uk
Halliday
Aileen M
AM
Parker
Lois
L
Smith
W David
WD
Kenyon
Fiona
F
Knox
David P
DP
Huntley
John F
JF
D000485:Allergens / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D000595:Amino Acid Sequence; D000818:Animals; D002392:Catechin / Q000235:genetics / Q000276:immunology; D018076:DNA, Complementary / Q000302:isolation & purification; D015870:Gene Expression; D005982:Glutathione Transferase / Q000235:genetics / Q000276:immunology; D008924:Mite Infestations / Q000517:prevention & control / Q000662:veterinary*; D008969:Molecular Sequence Data; D040901:Proteomics; D040042:Psoroptidae / Q000737:chemistry / Q000201:enzymology / Q000276:immunology*; D016415:Sequence Alignment / Q000662:veterinary; D012756:Sheep; D012757:Sheep Diseases / Q000469:parasitology / Q000517:prevention & control*; D014335:Tropomyosin / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology
D000485:Allergens; D018076:DNA, Complementary; D014335:Tropomyosin; D014614:Vaccines, Synthetic; D002392:Catechin; D005982:Glutathione Transferase