title: Comparison of distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma, or histologically normal urinary bladders.
abstract: OBJECTIVE
  To compare distributions of survivin among tissues from urinary bladders of dogs with cystitis, transitional cell carcinoma (TCC), or histologically normal urinary bladders.
  
  
  METHODS
  24 archived and 7 fresh-frozen specimens of urinary bladders from dogs with cystitis.
  
  
  METHODS
  Immunohistochemical analysis of archived tissue specimens was performed to identify survivin protein in the nucleus and cytoplasm of cells by use of polyclonal rabbit anti-survivin antibody. Tissues that contained > or = 5% immunoreactive cells were considered positive for survivin protein. Reverse-transcription PCR analysis was performed on fresh-frozen tissues to identify survivin mRNA. Data on tissues from dogs with TCC or histologically normal urinary bladders that were obtained during another study were used for statistical comparisons.
  
  
  RESULTS
  Twelve of 24 (50%) cystitic tissues were positive for nuclear survivin, compared with 28 of 41 (68%) TCC tissues and 0 of 46 (0%) normal tissues. Two of 24 (8%) cystitic tissues were positive for cytoplasmic survivin, compared with 7 of 41 (17%) TCC tissues and 17 of 46 (37%) normal tissues. Proportions of specimens that contained nuclear or cytoplasmic survivin were significantly different between cystitic and normal tissues but not between cystitic and TCC tissues. Four of 7 cystitic tissues were positive for survivin mRNA, which was comparable with results for TCC and normal tissues.
  
  
  CONCLUSIONS
  Nuclear survivin was detected in TCC and cystitic tissues but not in normal urinary bladder tissues. Additional studies are needed to determine whether nuclear survivin contributes to the development or progression of TCC.
authors.affiliation: Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Wendi Velando
authors.initials: WV
authors.lastname: Rankin
authors.forename: Carolyn J
authors.initials: CJ
authors.lastname: Henry
authors.forename: Susan E
authors.initials: SE
authors.lastname: Turnquist
authors.forename: James R
authors.initials: JR
authors.lastname: Turk
authors.forename: Marilyn E
authors.initials: ME
authors.lastname: Beissenherz
authors.forename: Jeffrey W
authors.initials: JW
authors.lastname: Tyler
authors.forename: Jonathan A
authors.initials: JA
authors.lastname: Green
mesh_terms: D000818:Animals; D002295:Carcinoma, Transitional Cell / Q000378:metabolism / Q000662:veterinary*; D002467:Cell Nucleus / Q000378:metabolism; D003556:Cystitis / Q000378:metabolism* / Q000662:veterinary*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D012016:Reference Values; D020133:Reverse Transcriptase Polymerase Chain Reaction; D001743:Urinary Bladder / Q000378:metabolism*; D001749:Urinary Bladder Neoplasms / Q000378:metabolism / Q000662:veterinary*
chemical_list: D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger