title: Steroidogenic capacity of Trypanosoma cruzi trypomastigotes.
abstract: American Trypanosomiasis is caused by the hemoflagellate Trypanosoma cruzi (T. cruzi) and affects millions of persons causing variable degrees of digestive and heart disturbances. As far as we concerned, T. cruzi capacity to synthesize steroid hormones has not been investigated. Therefore, the aim of this work was to investigate the capacity of T. cruzi trypomastigotes to transform tritiated steroid precursors into androgens and estrogens. The T. cruzi Tulahuén strain was obtained from mice blood. The trypomastigotes were cultured for 6 and 24h in Dulbbeco's modified Eagle's medium plus FCS and antibiotics. Tritiated dehydroepiandrosterone or androstendione were added to the culture media and parasites were incubated for 6 or 24h. The cultures were centrifuged and ether extracted. The steroids were analyzed by thin layer chromatography (TLC) in two solvent systems. After incubation with 3H-androstenedione, T. cruzi trypomastigotes synthesized 3H-testosterone (T), 3H-17beta-estradiol (E2) and 3H-estrone (E1). Metabolism of 3H-DHEA by the parasites yielded 3H-androstendione and 3H-androstendiol at 6h of incubation. The recrystallization procedure further demonstrated the 3H-androstendiol and 3H-17beta-estradiol syntheses. Results indicate for the first time that T. cruzi trypomastigotes produce androgens and estrogens when incubated in the presence of steroid precursors and suggest the presence of active parasite steroidogenic enzymes.
authors.affiliation: Instituto de Inmunologia, Facultad de Ciencias Médicas, UNR, Rosario, Argentina.
authors.forename: P
authors.initials: P
authors.lastname: Vacchina
authors.forename: R A
authors.initials: RA
authors.lastname: Valdéz
authors.forename: Y
authors.initials: Y
authors.lastname: Gómez
authors.forename: S
authors.initials: S
authors.lastname: Revelli
authors.forename: M C
authors.initials: MC
authors.lastname: Romano
mesh_terms: D000728:Androgens / Q000378:metabolism*; D000735:Androstenedione / Q000378:metabolism; D000818:Animals; D014355:Chagas Disease / Q000382:microbiology; D003687:Dehydroepiandrosterone / Q000378:metabolism; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D051379:Mice; D014349:Trypanosoma cruzi / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000728:Androgens; D004967:Estrogens; D000735:Androstenedione; D003687:Dehydroepiandrosterone