[Study of Tripterygium hypoglaucum Hutch on mouse's splenic lymphocyte activation in vitro].
OBJECTIVE
To study the effects of Tripterygium hypoglaucum Hutch (THH) on the mouse's splenic lymphocyte proliferation, activation mark such as CD69 and CD25, cytokine such as IL-4 and gamma-IFN.


METHODS
Separating the mouse's splenic lymphocyte sterilitily and adding THH of different density. The proliferation of the ConA-stimulated splenic lymphocyte was measured with MTT; the activation marks such as CD69 and CD25 were measured with flow cytometer; cytokines such as IL-4 and gamma-IFN were measured with euzymelinked immunosorbent assay.


RESULTS
THH had inhibitory action on both proliferation and activation of splenic lymphocyte.
Xu
Yan
Y
Department of Dermatology, First Affiliated Hospital of Kunming College, Kunming 650032, China. xuyanfirst@163.com
Zheng
Yong-Tang
YT
He
Li
L
Wan
Ping
P
Nong
Xiang
X
D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D013154:Spleen / Q000166:cytology*; D013997:Time Factors; D029757:Tripterygium / Q000737:chemistry*
D015703:Antigens, CD; D004365:Drugs, Chinese Herbal; D015847:Interleukin-4; D007371:Interferon-gamma