Simultaneous determination of triamterene and hydrochlorothiazide in tablets using derivative spectrophotometry.
A quick and accurate method for determining triamterene and hydrochlorothiazide in complex drugs of diuretic activity by using first-derivative (D1) and second-derivative (D2) spectrophotometry was developed. The zero-crossing technique was employed in measurements, using D1 at lambda = 240.9 nm and D2 at lambda= 278.2 nm for determining triamterene and D1 at lambda = 255.7 nm and D2 at lambda = 283.2 nm for hydrochlorothiazide. The linear relationship between the values of derivatives and analyte concentrations are maintained for concentrations from 2.40 microg x mL(-1) to 12.00 microg x mL(-1) for triamterene and from 1.25 microg x mL(-1) to 6.25 microg x mL(-1) for hydrochlorothiazide. LOD for triamterene was 0.90 microg x mL(-1) or 1.02 microg x mL(-1), while LOQ was 2.73 microg x mL(-1) or 3.08 microg x mL(-1). The corresponding values for hydrochlorothiazide were: LOD 0.25 microg x mL(-1) or 0.17 microg x mL(-1) and LOQ 0.77 microg x mL(-1) or 0.51 microg x mL(-1) depending on the derivative used. The determination results of drug constituents are of high accuracy, percentage recovery ranging from 97.17% to 99.74% for triamterene and from 102.44% to 102.64% for hydrochlorothiazide, and good precision. The computed values of RSD are smaller than 2.73% for triamterene and below 1.63% for hydrochlorothiazide. Selectivity and sensitivity of the developed method are satisfactory.
Stolarczyk
Mariusz
M
Jagiellonian University, Collegium Medicum, Department of Inorganic and Analytical Chemistry, Kraków, Poland. mstolar@cm-uj.krakow.pl
Apola
Anna
A
Krzek
Jan
J
Lech
Katarzyna
K
D004232:Diuretics / Q000032:analysis*; D004338:Drug Combinations; D006852:Hydrochlorothiazide / Q000032:analysis*; D015203:Reproducibility of Results; D013053:Spectrophotometry / Q000379:methods*; D013607:Tablets; D014223:Triamterene / Q000032:analysis*
D004232:Diuretics; D004338:Drug Combinations; D013607:Tablets; D006852:Hydrochlorothiazide; D014223:Triamterene