title: Mutator genes giving rise to decreased antibiotic susceptibility in Pseudomonas aeruginosa.
abstract: Screening of the PA14 genomic transposon mutant library for resistance to ceftazidime, tobramycin, and ciprofloxacin led to the discovery of several mutants that appeared in more than one screen. Testing of the frequency of mutation to ciprofloxacin resistance revealed previously known mutator genes, including mutS and mutL, as well as mutators that have not yet been described for P. aeruginosa, including PA3958 and RadA (PA4609).
authors.affiliation: Centre for Microbial Diseases and Immunity Research, 2259 Lower Mall Research Station, University of British Columbia, Vancouver, British Columbia, Canada.
authors.forename: Irith
authors.initials: I
authors.lastname: Wiegand
authors.forename: Alexandra K
authors.initials: AK
authors.lastname: Marr
authors.forename: Elena B M
authors.initials: EB
authors.lastname: Breidenstein
authors.forename: Kristen N
authors.initials: KN
authors.lastname: Schurek
authors.forename: Patrick
authors.initials: P
authors.lastname: Taylor
authors.forename: Robert E W
authors.initials: RE
authors.lastname: Hancock
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002939:Ciprofloxacin / Q000494:pharmacology; D003550:Cystic Fibrosis / Q000150:complications / Q000188:drug therapy / Q000382:microbiology; D004260:DNA Repair / Q000235:genetics; D004251:DNA Transposable Elements / Q000235:genetics; D004269:DNA, Bacterial / Q000235:genetics; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D005798:Genes, Bacterial; D015698:Genomic Library; D006801:Humans; D009154:Mutation; D010641:Phenotype; D011552:Pseudomonas Infections / Q000150:complications / Q000188:drug therapy / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D004251:DNA Transposable Elements; D004269:DNA, Bacterial; D002939:Ciprofloxacin