Cloning and functional characterization of chicken interleukin-17D.
The chicken interleukin-17D was cloned from a testis cDNA library prepared from the Korean native chicken. The full-length chicken IL-17D (chIL-17D) cDNA consisted of a 348 nucleotide sequence encoding an open reading frame of 116 amino acids with a predicted molecular mass of 13.3kDa. Comparison of the deduced amino acid sequence of chIL-17D with homologous proteins from human, mouse and opossum revealed 64%, 53% and 76% identity, respectively, including six conserved cysteine residues present in the mammalian polypeptides. The chIL-17D gene transcript was expressed in a wide range of tissues, and highest levels were in pancreas, thymus and lung. Following Eimeria maxima infection, levels of the chIL-17D mRNA were up-regulated in the intestinal jejunum, bursa, lung, and spleen but decreased in the thymus. Infected chickens also expressed greater levels of chIL-17D mRNA in CD4(+), CD8(+) and TCR1(+) intestinal intraepithelial lymphocytes while decreased expression was seen in TCR2(+) cells. Treatment of CHCC-OU2 fibroblasts with chIL-17D recombinant protein induced the expression of IL-6 and IL-8. Collectively, these results suggest that chL-17D has structural and functional similarities to mammalian IL-17Ds and that it plays an important role in local gut innate immune responses during experimental coccidiosis.
Hong
Yeong Ho
YH
Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Building 1040, BARC-East, U.S. Department of Agriculture, Beltsville, MD 20705, USA.
Lillehoj
Hyun S
HS
Park
Dong Woon
DW
Lee
Sung Hyen
SH
Han
Jae Yong
JY
Shin
Ji Hye
JH
Park
Myeung Sun
MS
Kim
Jin-Kyoo
JK
D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D002645:Chickens / Q000276:immunology / Q000378:metabolism*; D003001:Cloning, Molecular; D003048:Coccidiosis / Q000276:immunology / Q000662:veterinary; D018076:DNA, Complementary / Q000235:genetics; D016054:DNA, Protozoan / Q000235:genetics; D004539:Eimeria; D020381:Interleukin-17 / Q000235:genetics* / Q000378:metabolism*; D008969:Molecular Sequence Data; D011201:Poultry Diseases / Q000276:immunology; D012333:RNA, Messenger / Q000378:metabolism; D016053:RNA, Protozoan / Q000378:metabolism
D018076:DNA, Complementary; D016054:DNA, Protozoan; D020381:Interleukin-17; D012333:RNA, Messenger; D016053:RNA, Protozoan