A tentative mechanism of solubilization of neuropathy target esterase from chicken embryo brain by phospholipase A2.
The neuropathy target esterase is a membrane-bound enzyme linked to organophosphate-induced distal neuropathy. Here we report a tentative mechanism of its solubilization from chicken embryo brains by using phospholipase A2. The enzyme was released from brain membranes after degradation of their structural phospholipids initiated by phospholipase A2. L-A inverted exclamation mark-lysophosphatidylcholine, tested as a representative product of phospholipid hydrolysis, was identified as a new efficient detergent for solubilization of the neuropathy target esterase.
Seifert
Josef
J
Department of Plant and Environmental Protection Sciences, University of Hawaii, Honolulu, HI 96822, USA. josef@hawaii.edu
D000818:Animals; D001921:Brain / Q000187:drug effects / Q000196:embryology / Q000201:enzymology*; D002265:Carboxylic Ester Hydrolases / Q000378:metabolism*; D002642:Chick Embryo; D002855:Chromatography, Thin Layer; D008244:Lysophosphatidylcholines / Q000494:pharmacology; D054467:Phospholipases A2 / Q000378:metabolism*; D012995:Solubility
D008244:Lysophosphatidylcholines; D002265:Carboxylic Ester Hydrolases; C021859:neurotoxic esterase; D054467:Phospholipases A2