title: A murine intraperitoneal infection model reveals that host resistance to Campylobacter jejuni is Nramp1 dependent.
abstract: We tested the hypothesis that host resistance to Campylobacter jejuni is Nramp1 dependent. Following intraperitoneal (IP) inoculation of Nramp1+/+ and isogenic Nramp1-deficient (Nramp1-/-) mice C. jejuni primarily associated with mac1-positive cells in liver tissue. A significant reduction of C. jejuni was observed in Nramp1+/+ mice 4 days post-infection (PI) (liver) and 8 days PI cecum-colon. In contrast, Nramp1-/- mice showed no significant reduction of C. jejuni and instead had a chronic inflammatory response and significant histopathological lesions 30 days PI. Differential cytokine profiles were observed in C. jejuni infected Nramp1+/+ and Nramp1-/- primary dendritic cells. Taken together these data indicate that Nramp1 is critical for host resistance to C. jejuni.
authors.affiliation: University of British Columbia, Department of Microbiology and Immunology, Life Sciences Centre, 2558-2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: Olivia L
authors.initials: OL
authors.lastname: Champion
authors.forename: Yanet
authors.initials: Y
authors.lastname: Valdez
authors.forename: Lisa
authors.initials: L
authors.lastname: Thorson
authors.forename: Julian A
authors.initials: JA
authors.lastname: Guttman
authors.forename: Alfredo
authors.initials: A
authors.lastname: Menendez
authors.forename: Erin C
authors.initials: EC
authors.lastname: Gaynor
authors.forename: B Brett
authors.initials: BB
authors.lastname: Finlay
mesh_terms: D000818:Animals; D016123:Campylobacter jejuni / Q000276:immunology*; D027682:Cation Transport Proteins / Q000276:immunology*; D002432:Cecum / Q000382:microbiology; D015169:Colony Count, Microbial; D005260:Female; D007113:Immunity, Innate; D007249:Inflammation / Q000473:pathology; D008099:Liver / Q000382:microbiology / Q000473:pathology; D008198:Lymph Nodes / Q000382:microbiology; D051379:Mice; D018345:Mice, Knockout; D013154:Spleen / Q000382:microbiology; D000099173:Natural Resistance-associated Macrophage Protein 1
chemical_list: D027682:Cation Transport Proteins; D000099173:Natural Resistance-associated Macrophage Protein 1