Apoptotic effects on B-cell chronic lymphocytic leukemia (B-CLL) cells of heterocyclic compounds isolated from Guttiferaes.
A series of 10 heterocyclic compounds purified from Allanblackia were tested on two B cell lines, ESKOL and EHEB, and on cells from B-CLL patients. Several molecules inhibited the proliferation of both cell lines and promoted apoptosis of B-CLL cells through different mechanisms, some of them elicited a dissipation of the mitochondrial transmembrane potential, other triggered caspase-3 activation and cleavage of the inducible nitric oxide synthase. Blood mononuclear cells and B-lymphocytes from healthy donors appeared less sensitive than B-CLL cells. These results indicate that these molecules may be of interest in the development of new therapies for B-CLL.
Menasria
F
F
UMRS 872 INSERM/Université Pierre et Marie Curie/Université Paris Descartes, Centre de Recherche des Cordeliers, Paris, France.
Azebaze
A G B
AG
Billard
C
C
Faussat
A M
AM
Nkengfack
A E
AE
Meyer
M
M
Kolb
J P
JP
D000368:Aged; D000369:Aged, 80 and over; D053148:Caspase 3 / Q000187:drug effects / Q000378:metabolism; D002455:Cell Division / Q000187:drug effects; D005260:Female; D005500:Follow-Up Studies; D006571:Heterocyclic Compounds / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000473:pathology*; D008297:Male; D029581:Malpighiaceae / Q000737:chemistry*; D008875:Middle Aged; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000502:physiology; D010539:Permeability / Q000187:drug effects; D018517:Plant Roots / Q000737:chemistry; D014407:Tumor Cells, Cultured; D044004:Xanthones / Q000302:isolation & purification / Q000494:pharmacology*
D006571:Heterocyclic Compounds; D044004:Xanthones; D053148:Caspase 3; C021053:mangostin