Isolation of dibenzofuran-degrading bacterium, Nocardioides sp. DF412, and characterization of its dibenzofuran degradation genes.
A dibenzofuran (DF)-degrading bacterium, Nocardioides sp. DF412, was isolated and its genes responsible for the degradation of DF were cloned and characterized. The dfdA1A2A3A4 gene cluster coding for a ring hydroxylating dioxygenase was located on a plasmid, and the dfdBC gene cluster coding for a ring-cleavage dioxygenase and a following hydrolase respectively was located on the chromosome. Reverse transcription-PCR analysis revealed that the transcription of both gene clusters was induced in the presence of DF. These two gene clusters also conferred the activity of each enzyme to a host strain. These results strongly suggest that the clusters are responsible for the degradation of DF in strain DF412.
Miyauchi
Keisuke
K
Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Niigata, Japan. kmiya@tjcc.tohoku-gakuin.ac.jp
Sukda
Parichat
P
Nishida
Tetsuya
T
Ito
Emi
E
Matsumoto
Yoshikatsu
Y
Masai
Eiji
E
Fukuda
Masao
M
D000192:Actinomycetales / Q000235:genetics* / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism*; D001572:Benzofurans / Q000378:metabolism*; D001673:Biodegradation, Environmental
D001426:Bacterial Proteins; D001572:Benzofurans; C023614:dibenzofuran