Antibody-mediated enhancement of the rate, magnitude, and responsiveness of vesicular stomatitis virus induced alpha interferon production.
A majority of adults without evidence of exposure to vesicular stomatitis virus (VSV) have serum IgG antibodies that interact with pro-inflammatory TLR7 in the presence of VSV, and enhance several aspects of VSV-induced IFN-alpha production. Enhancing IgG antibody enables human PBMC to make IFN-alpha more rapidly and in higher titers in response to a broad range of VSV-concentrations that include those too low to independently stimulate IFN-alpha production. These antibody-mediated functions compensate for the inherent delay in virus-induced IFN-alpha production in vitro, and have the potential to improve the in vivo IFN-alpha response and effectively terminate infection before the occurrence of clinically apparent disease. The frequent presence of enhancing antibody in persons without predictable VSV exposure has implications for naturally occurring infections with this and other viruses, and for the use of viruses as vaccine vectors and oncolytic agents.
Green
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Department of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA.
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Mullins
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Green
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D000328:Adult; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology*; D019067:Antibody-Dependent Enhancement; D006801:Humans; D016898:Interferon-alpha / Q000096:biosynthesis*; D054243:Vesicular Stomatitis / Q000276:immunology*; D018116:Vesiculovirus / Q000276:immunology*
D000914:Antibodies, Viral; D016898:Interferon-alpha