Effects of enoxaparin and unfractionated heparin on platelet activity and reactivity during carotid endarterectomy.
The aim of this study was to determine platelet activity and reactivity and the effects of unfractionated heparin (UFH) and enoxaparin on platelet function during carotid eversion endarterectomy under local anesthesia. Twenty symptomatic patients undergoing carotid endarterectomy were randomly assigned to either 5,000 units of UFH or body weight-adjusted enoxaparin (0.5 mg/kg body weight) as an intraoperative intravenous bolus. The activity of platelets was assessed by measuring the expression of CD62p and CD41 with flow cytometry. Additionally, platelet-leukocyte aggregates (PLAs) were enumerated. The reactivity of platelets was evaluated by measuring the expression of the same antigens after stimulation. In addition, platelet reactivity was also analyzed using a PFA-100 analyzer. A significant increase in platelet activity was observed during surgery for CD41 and CD62p (p = .002 and < .001, respectively). The number of PLAs showed no significant changes during surgery. Yet there was a significant difference between patients treated with UFH and patients treated with enoxaparin. No difference for platelet activity or reactivity for patients receiving either UFH or enoxaparin prior to cross-clamping of the carotid arteries was seen. The formation of PLAs after endarterectomy was significantly higher in the UFH group; thus, PLAs are probably a useful surrogate parameter for measuring platelet activity.
Assadian
Afshin
A
Department of General and Vascular Surgery, Wilhelminenspital Vienna, Austria. afshin_assadian@yahoo.de
Senekowitsch
Christian
C
Hagmüller
Georg W
GW
Lax
Jaqueline
J
Hübl
Wolfgang
W
D000368:Aged; D000772:Anesthesia, Local; D000925:Anticoagulants / Q000494:pharmacology*; D001792:Blood Platelets / Q000187:drug effects*; D002343:Carotid Artery, Internal / Q000601:surgery; D016893:Carotid Stenosis / Q000097:blood / Q000601:surgery; D016894:Endarterectomy, Carotid; D017984:Enoxaparin / Q000494:pharmacology; D005260:Female; D006493:Heparin / Q000494:pharmacology*; D006801:Humans; D007430:Intraoperative Care / Q000379:methods; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000097:blood; D010974:Platelet Aggregation / Q000187:drug effects; D010979:Platelet Function Tests / Q000379:methods; D040201:Platelet Membrane Glycoprotein IIb / Q000097:blood
D000925:Anticoagulants; D017984:Enoxaparin; D019007:P-Selectin; D040201:Platelet Membrane Glycoprotein IIb; D006493:Heparin