Microencapsulation of a probiotic bacteria with alginate-gelatin and its properties.
Lactobacillus casei ATCC 393-loaded microcapsules based on alginate and gelatin had been prepared by extrusion method and the product could increase the cell numbers of L. casei ATCC 393 to be 10(7) CFU g(-1) in the dry state of microcapsules. The microparticles homogeneously distributed with size of 1.1 ± 0.2 mm. Four kinds of microcapsules (S(1), S(2), S(3) and S(4)) exhibited swelling in simulated gastric fluid (SGF) while the beads eroded and disintegrated rapidly in simulated intestinal fluid (SIF). Cells of L. casei ATCC 393 could be continuously released from the microcapsules during simulated gastrointestinal tract (GIT) and the release amounts and speeds in SIF were much higher and faster than that in SGF. Encapsulation in alginate-gelatin microcapsules successfully improved the survival of L. casei ATCC 393 and this approach might be useful in delivery of probiotic cultures as a functional food.
Li
Xiao Yan
XY
College of Marine Life Science, Ocean University of China, Qingdao, P R China.
Chen
Xi Guang
XG
Cha
Dong Su
DS
Park
Hyun Jin
HJ
Liu
Cheng Sheng
CS
D000464:Alginates / Q000737:chemistry*; D002214:Capsules; D004339:Drug Compounding / Q000379:methods*; D005766:Gastrointestinal Contents / Q000737:chemistry; D005780:Gelatin / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007780:Lacticaseibacillus casei / Q000166:cytology*; D010316:Particle Size; D019936:Probiotics / Q000008:administration & dosage*
D000464:Alginates; D002214:Capsules; D006603:Hexuronic Acids; D020723:Glucuronic Acid; D005780:Gelatin