title: [Noninvasive fetal sex detection from maternal plasma in pregnant women].
abstract: OBJECTIVE
  Objective of our study was optimization of noninvasive fetal sex detection from maternal plasma in pregnant women.
  
  
  METHODS
  Molecular DNA quantitative analyses in gonosomal loci.
  
  
  METHODS
  The study was performed at Department of Medical Genetics and Fetal Medicine, University Hospital Olomouc.
  
  
  METHODS
  Together 475 DNA samples isolated from maternal plasma in different weeks of pregnancy ranging from 4th w.g. to 37th w.g. Y chromosomal sequences in AMELY and TSPY were tested by refined quantitative fluorescent PCR using capillary electrophoresis.
  
  
  RESULTS
  The method is able to distinguish 1% of Y chromosomal sequences of artificial mixtures. Investigation and assessment in cell free fetal DNA samples achieved 4.05% of false positivity and 7.15% of false negativity in Y sequence detection.
  
  
  CONCLUSIONS
  Established method allows detecting fetal sex with high sensitivity and specificity. The method is possible to use also for quantitative purposes.
authors.affiliation: Ustav lékarské genetiky a fetální medicíny, Fakultnńemocnice Olomouc.
authors.forename: R
authors.initials: R
authors.lastname: Vodicka
authors.forename: R
authors.initials: R
authors.lastname: Vrtĕl
authors.forename: E
authors.initials: E
authors.lastname: Schneiderová
authors.forename: D
authors.initials: D
authors.lastname: Vrbická
authors.forename: M
authors.initials: M
authors.lastname: Procházka
authors.forename: I
authors.initials: I
authors.lastname: Dhaifalah
authors.forename: A
authors.initials: A
authors.lastname: Santavá
authors.forename: J
authors.initials: J
authors.lastname: Santavý
mesh_terms: D041322:Chromosomes, Human, Y / Q000235:genetics*; D004247:DNA / Q000097:blood; D019075:Electrophoresis, Capillary; D005260:Female; D006801:Humans; D016133:Polymerase Chain Reaction; D011247:Pregnancy; D017422:Sequence Analysis, DNA; D012732:Sex Determination Analysis / Q000379:methods*
chemical_list: D004247:DNA