Rosmarinic acid protects human dopaminergic neuronal cells against hydrogen peroxide-induced apoptosis.
In this study, we investigated the protective effects of rosmarinic acid (RA) on H(2)O(2)-induced neurotoxicity in human dopaminergic cell line, SH-SY5Y. Results showed that RA significantly attenuated H(2)O(2)-induced reactive oxygen species (ROS) generation and apoptotic cell death. Rosmarinic acid effectively suppressed the up-regulation of Bax and down-regulation of Bcl-2. Furthermore, RA stimulated the antioxidant enzyme heme oxygenase-1 (HO-1). We also demonstrated that the HO-1 induction by RA was associated with the protein kinase A (PKA) and phosphatidylinositiol-3-kinase (PI3K) signaling pathways. These results suggest that RA can protect SH-SY5Y cells under oxidative stress conditions by regulating apoptotic process. Thus, RA should be clinically evaluated for the prevention of neurodegenerative diseases.
Lee
Hyo Jung
HJ
Department of Biotechnology (BK21 Program), Chosun University, Gwangju 501-759, Republic of Korea.
Cho
Hong-Suk
HS
Park
Euteum
E
Kim
Seung
S
Lee
Sook-Young
SY
Kim
Chun-Sung
CS
Kim
Do Kyung
DK
Kim
Sung-Jun
SJ
Chun
Hong Sung
HS
D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects / Q000648:ultrastructure; D002934:Cinnamates / Q000494:pharmacology*; D017868:Cyclic AMP-Dependent Protein Kinases / Q000378:metabolism; D053630:Depsides / Q000494:pharmacology*; D004298:Dopamine / Q000502:physiology*; D015870:Gene Expression / Q000187:drug effects; D019254:Genes, bcl-2 / Q000187:drug effects; D051547:Heme Oxygenase-1 / Q000096:biosynthesis; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors* / Q000633:toxicity*; D009474:Neurons / Q000187:drug effects / Q000502:physiology*; D016877:Oxidants / Q000633:toxicity*; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D051028:bcl-2-Associated X Protein / Q000096:biosynthesis / Q000235:genetics; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism; D000097123:Rosmarinic Acid
D000975:Antioxidants; D053148:Caspase 3; D002934:Cinnamates; D017868:Cyclic AMP-Dependent Protein Kinases; D053630:Depsides; D004298:Dopamine; D051547:Heme Oxygenase-1; D006861:Hydrogen Peroxide; D016877:Oxidants; D019869:Phosphatidylinositol 3-Kinases; D017382:Reactive Oxygen Species; D051028:bcl-2-Associated X Protein; D048051:p38 Mitogen-Activated Protein Kinases; D000097123:Rosmarinic Acid