title: Cooperative kinetics of the recombinant glutathione transferase of Taenia solium and characterization of the enzyme.
abstract: Glutathione transferases (GSTs) are essential enzymes in many organisms due their diverse functions and, in helminths they are the main detoxification system. For Taenia solium, two cytosolic GSTs with molecular masses of 25.5 and 26.5 kDa (Ts26GST) have been found. Ts26GST was cloned to be studied in its recombinant form (recTs26GST). Although the primary structure is related to the mu class, the kinetic parameters for CDNB (V(max)=51.5 micromol min(-1)mg(-1); K(m)=1.06 mM; k(cat)= 22.2s(-1)) are related with some alpha GSTs. The substrate and inhibitor class markers reaffirmed these bimodal characteristics. Inhibition studies with anthelminthics indicate that recTs26GST is sensitive to mebendazole, displaying a non competitive inhibition pattern suggesting that at least two molecules are binding to recTs26GST. On the other hand, the kinetic curves for CDNB and GSH showed a positive cooperativity that was corroborated using fluorometric assays. Those assays indicate that CDNB binding is highly influenced by GSH, probably by modulation of the Ts26GST conformational ensamble.
authors.affiliation: Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Edificio A, 2 degrees piso, Ciudad Universitaria, Mexico DF 04510, Mexico.
authors.forename: Anayetzin
authors.initials: A
authors.lastname: Torres-Rivera
authors.forename: Abraham
authors.initials: A
authors.lastname: Landa
mesh_terms: D000818:Animals; D004789:Enzyme Activation; D005982:Glutathione Transferase / Q000737:chemistry*; D007700:Kinetics; D011994:Recombinant Proteins / Q000737:chemistry*; D041201:Taenia solium / Q000201:enzymology* / Q000235:genetics
chemical_list: D011994:Recombinant Proteins; D005982:Glutathione Transferase