Plasminogen-dependent proteolytic activity in Bifidobacterium lactis.
Bifidobacteria represent one of the most important health-promoting bacterial groups of the intestinal microbiota. The binding of plasminogen to species of Bifidobacterium has been recently reported. To further explore the interaction between bifidobacteria and plasminogen, we investigated the role of Bifidobacterium lactis BI07 plasminogen-dependent proteolytic activity in the degradation of host-specific substrates. Our experimental data demonstrate that the recruitment of plasminogen on the bacterial cell surface and its subsequent conversion into plasmin by host-derived plasminogen activators provide B. lactis BI07 with a surface-associated plasmin activity effective in degradation of physiological substrates such as extracellular matrix, fibronectin and fibrinogen. The ability of bifidobacteria to intervene in the host plasminogen/plasmin system may contribute to facilitating colonization of the host gastrointestinal tract.
Candela
Marco
M
Department of Pharmaceutical Sciences, CIRB-centre for Biotechnology, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Miccoli
Giacomo
G
Department of Pharmaceutical Sciences, CIRB-centre for Biotechnology, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Bergmann
Simone
S
Max von Pettenkofer Institut, Ludwig-Maximilians-Universität München, Pettenkoferstr. 9a, D-80336 München, Germany.|University of Würzburg, Research Centre for Infectious Diseases, RÖntgenring 11, D-97070 Würzburg, Germany.
Turroni
Silvia
S
Department of Pharmaceutical Sciences, CIRB-centre for Biotechnology, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Vitali
Beatrice
B
Department of Pharmaceutical Sciences, CIRB-centre for Biotechnology, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
Hammerschmidt
Sven
S
Max von Pettenkofer Institut, Ludwig-Maximilians-Universität München, Pettenkoferstr. 9a, D-80336 München, Germany.|University of Würzburg, Research Centre for Infectious Diseases, RÖntgenring 11, D-97070 Würzburg, Germany.
Brigidi
Patrizia
P
Department of Pharmaceutical Sciences, CIRB-centre for Biotechnology, University of Bologna, Via Belmeloro 6, 40126 Bologna, Italy.
D001644:Bifidobacterium / Q000378:metabolism*; D045744:Cell Line, Tumor; D005109:Extracellular Matrix / Q000378:metabolism; D005340:Fibrinogen / Q000378:metabolism; D005341:Fibrinolysin / Q000378:metabolism; D005353:Fibronectins / Q000378:metabolism; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D010958:Plasminogen / Q000378:metabolism*; D010960:Plasminogen Activators / Q000378:metabolism; D011499:Protein Processing, Post-Translational
D005353:Fibronectins; D005340:Fibrinogen; D010958:Plasminogen; D010960:Plasminogen Activators; D005341:Fibrinolysin