Separation-free detection of nuclear factor kappa B with double-stranded molecular probes.
This paper reports a simple, rapid molecular binding scheme for the detection and quantification of the transcription factor nuclear factor kappa B and other DNA binding proteins without any separation or immobilization step.
Wang
Zhaohui
Z
Department of Aerospace and Mechanical Engineering, University of Arizona, Tucson, Arizona, USA.
Gidwani
Vinay
V
Zhang
Donna D
DD
Wong
Pak Kin
PK
D000818:Animals; D004247:DNA; D004268:DNA-Binding Proteins / Q000032:analysis; D005456:Fluorescent Dyes; D006801:Humans; D008856:Microscopy, Fluorescence; D015335:Molecular Probes; D016328:NF-kappa B / Q000032:analysis*; D011485:Protein Binding
D004268:DNA-Binding Proteins; D005456:Fluorescent Dyes; D015335:Molecular Probes; D016328:NF-kappa B; D004247:DNA