Electrophysiological neurodifferentiation of subventricular zone-derived precursor cells following stroke.
Stroke in rodents is associated with increased neurogenesis and the migration of newborn neurons to sites of brain ischemia, where they may participate in repair and recovery. To determine if neurogenesis following stroke yields functional new neurons, we labeled neuronal precursors in the mouse subventricular zone (SVZ) with a lentivirus-green fluorescent protein vector, produced stroke by occluding the middle cerebral artery, and detected newborn neurons 8 weeks later by fluorescence microscopy. Patch-clamp studies on fluorescent neurons in the cortical region surrounding infarction showed tetrodotoxin-sensitive Na(+) action potentials and spontaneous excitatory post-synaptic currents, suggesting that ischemia led to functional neurogenesis with synaptic integration. These findings support the hypothesis that enhancing endogenous neurogenesis after stroke might have therapeutic benefit.
Lai
Bin
B
Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, CA 94945, United States.
Mao
Xiao Ou
XO
Xie
Lin
L
Jin
Kunlin
K
Greenberg
David A
DA
D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D000089622:Doublecortin Domain Proteins; D049452:Green Fluorescent Proteins / Q000235:genetics; D008856:Microscopy, Fluorescence; D008869:Microtubule-Associated Proteins / Q000096:biosynthesis; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D009479:Neuropeptides / Q000096:biosynthesis; D018408:Patch-Clamp Techniques; D051381:Rats; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism*; D020521:Stroke / Q000473:pathology / Q000503:physiopathology*
D000089622:Doublecortin Domain Proteins; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D049452:Green Fluorescent Proteins