title: Characterization of Clostridium difficile strains isolated from patients in Ontario, Canada, from 2004 to 2006.
abstract: Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated from persons in Ontario, Canada, during 2004 to 2006 according to toxin type (enterotoxin A, cytotoxin B, and binary toxin [CDT]), tcdC gene characterization, ribotyping, pulsed-field gel electrophoresis, and toxinotyping. Clostridium difficile was isolated from 1,080/1,152 (94%) samples from 21 diagnostic laboratories. Isolates with toxin profiles A(+) B(+) CDT(-), A(+) B(+) CDT(+), A(-) B(+) CDT(-), and A(-) B(+) CDT(+) accounted for 63%, 34%, 2.4%, and 0.6% of isolates, respectively. Alterations in tcdC were detected in six different ribotypes, including ribotype 027. A total of 39 different ribotypes were identified, with ribotype 027/North American pulsotype 1 (NAP1), an internationally recognized outbreak strain associated with severe disease, being the second most common ribotype (19% of isolates). Transient resistance to metronidazole was identified in 19 (1.8%) isolates. While a large number of ribotypes were found, a few predominated across the province. The high prevalence and wide distribution of ribotype 027/NAP1 are disconcerting in view of the severity of disease associated with it.
authors.affiliation: Department of Clinical Studies, University of Guelph, Guelph, Ontario, Canada.
authors.forename: H
authors.initials: H
authors.lastname: Martin
authors.forename: B
authors.initials: B
authors.lastname: Willey
authors.forename: D E
authors.initials: DE
authors.lastname: Low
authors.forename: H R
authors.initials: HR
authors.lastname: Staempfli
authors.forename: A
authors.initials: A
authors.lastname: McGeer
authors.forename: P
authors.initials: P
authors.lastname: Boerlin
authors.forename: M
authors.initials: M
authors.lastname: Mulvey
authors.forename: J S
authors.initials: JS
authors.lastname: Weese
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D016360:Clostridioides difficile / Q000235:genetics; D024881:Drug Resistance, Bacterial; D004761:Enterocolitis, Pseudomembranous / Q000382:microbiology*; D004768:Enterotoxins; D005260:Female; D005838:Genotype; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D009864:Ontario; D016133:Polymerase Chain Reaction; D021521:Ribotyping; D055815:Young Adult
chemical_list: D004768:Enterotoxins