Recent advances in DNA sensors.
The concept of DNA biosensors is sustained by the need for rapid and highly sensitive analytical tools for genetic detection. Their implementation is based on three key steps: (i) immobilization of single-stranded oligonucleotide probes onto a substrate; (ii) hybridization and (iii) reading. These steps involve complementary knowledge in various disciplinary fields such as surface physics and chemistry, molecular electrochemistry, micro-technologies, optics, electronics and biochemistry. We present here, in a non-exhaustive way, the recent advances in the two steps of immobilization and detection that rely upon increasing integration of the number of reading points or/and of the reading strategy.
Cosnier
Serge
S
Département de Chimie Moléculaire, UMR-5250, ICMG FR-2607, CNRS, Université Joseph Fourier BP 53, 38041 Grenoble Cédex 9, France. serge.cosnier@ujf-grenoble.fr
Mailley
Pascal
P
D000818:Animals; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis
D015342:DNA Probes; D004247:DNA