title: [Effects of Chinese herb compound Naoyikang on expression of choline acetyltransferase in brain of rats with Alzheimer's disease].
abstract: OBJECTIVE
  To observe the effects of Naoyikang (NYK) on expression of choline acetyltransferase (ChAT) in brain of rats with Alzheimer' s disease (AD).
  
  
  METHODS
  Bilateral infusions of Ibotenic acid (IBO) into nucleus basalis of Meynert (NBM) using hamilton syringe and stereotaxic apparatus were adopted to establish the rat model of AD. After intragastrically administrated with different solution for 28 days, immunohistochemistry and Western-blot were adopted to study the expression of ChAT in frontal cortex of AD rats.
  
  
  RESULTS
  NYK could improve the morphology and increase the number of ChAT immunoreactive neurons, and significantly promote ChAT protein expression.
  
  
  CONCLUSIONS
  NYK may be able to increase the synthesis of acetylcholine (ACh) through elevating the expression of ChAT protein, thus improving the level of brain ACh so as to protect central cholinergic neurons.
authors.affiliation: Medical School of Nantong University, Nantong 226001, China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Geng
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Zhou
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Shi
authors.forename: Ya-Er
authors.initials: YE
authors.lastname: Hu
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Mao
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000544:Alzheimer Disease / Q000201:enzymology*; D000818:Animals; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects* / Q000201:enzymology*; D002795:Choline O-Acetyltransferase / Q000378:metabolism*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007150:Immunohistochemistry; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D004365:Drugs, Chinese Herbal; D002795:Choline O-Acetyltransferase