High serum levels of thrombospondin-1 in patients with idiopathic interstitial pneumonia.
Thrombospondin-1 (TSP-1), a multifunctional matricellular glycoprotein, can activate transforming growth factor-beta, an important profibrotic cytokine involved in various fibrotic diseases. TSP-1 is expressed in the lung tissue of animal models of bleomycin-induced pulmonary fibrosis and in patients with some interstitial lung diseases. The present study investigated the serum and bronchoalveolar lavage fluid (BALF) levels of TSP-1 in the idiopathic interstitial pneumonias (IIPs) and the relationship between these levels and other clinical factors. The TSP-1 in the serum and BALF were measured in 45 patients with pathologically diagnosed IIPs [22 with usual interstitial pneumonia (UIP), 23 patients with non-specific interstitial pneumonia (NSIP)], as well as in 28 patients with pulmonary sarcoidosis and 15 healthy volunteers using a competitive enzyme immunoassay. The expression and localization of TSP-1 in the lungs were analyzed by immunohistochemical staining. The serum TSP-1 levels were significantly higher in patients with IIPs than in either those with sarcoidosis or the controls. These levels correlated well with those of an angiogenic cytokine vascular endothelial growth factor while correlating inversely with the %VC. Positive immunostaining of TSP-1 was predominantly observed in the regenerated alveolar epithelium and alveolar macrophages in the lung. Our findings suggest that the circulating TSP-1 levels are associated with the presence of interstitial pneumonia, but further studies are required before we can definitively conclude that TSP-1 plays a role in the pathogenesis of these diseases.
Ide
Mioko
M
Second Department of Internal Medicine, Nagasaki University School of Medicine, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.
Ishii
Hiroshi
H
Mukae
Hiroshi
H
Iwata
Atsuko
A
Sakamoto
Noriho
N
Kadota
Jun-ichi
J
Kohno
Shigeru
S
D000293:Adolescent; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002985:Clinical Protocols; D005260:Female; D006801:Humans; D054988:Idiopathic Interstitial Pneumonias / Q000378:metabolism* / Q000503:physiopathology; D007150:Immunohistochemistry / Q000379:methods; D008168:Lung / Q000166:cytology / Q000473:pathology; D016676:Macrophages, Alveolar / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D019700:Thrombospondin 1 / Q000097:blood / Q000378:metabolism*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D055815:Young Adult
D015415:Biomarkers; D019700:Thrombospondin 1; D016212:Transforming Growth Factor beta