title: Cellular effects of long wavelength UV light (UVA) in mammalian cells.
abstract: UVA should receive significant consideration as a human health risk as it is a large proportion of the solar spectrum that reaches the earth's surface and because of its ability to penetrate human skin. It is only relatively recently that this has been recognized and this previously under-researched part of the UV spectrum is becoming increasingly well characterized at doses that are quite low in relation to those experienced by humans. Absorption of UVA in a cell leads to the production of reactive oxygen and nitrogen species that can damage major biomolecules including DNA and membrane lipids. Various types of damage induced in these molecules lead to significant biological effects including cytotoxicity, mutations and alterations in cell signalling pathways. Longer-term effects such as persistent genomic instability and bystander effects have also been observed following UVA treatment of mammalian cells and, as with ionizing radiation, this changes some of the fundamental thinking around tissue effects of irradiation. Antioxidants have been assessed extensively for their ability to protect against the biological effects of UVA and a number have been shown to be successful at least in-vitro, for example vitamin E and epigallocatechin-3-gallate. Other potential targets for protection are suggested through the increased understanding of some of the signalling mechanisms activated following treatment, for example the inhibition of NADPH oxidase is seen to reduce a bystander effect. The search for appropriate and successful photoprotective agents remains an important area of research.
authors.affiliation: Biomedical Sciences Unit, Department of Biological Sciences, Lancaster University Lancaster, LA1 4YQ, UK. t.mcmillan@lancaster.ac.uk
authors.forename: T J
authors.initials: TJ
authors.lastname: McMillan
authors.forename: E
authors.initials: E
authors.lastname: Leatherman
authors.forename: A
authors.initials: A
authors.lastname: Ridley
authors.forename: J
authors.initials: J
authors.lastname: Shorrocks
authors.forename: S E
authors.initials: SE
authors.lastname: Tobi
authors.forename: J R
authors.initials: JR
authors.lastname: Whiteside
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D024201:Bystander Effect; D016923:Cell Death / Q000528:radiation effects; D004247:DNA / Q000528:radiation effects; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D042822:Genomic Instability; D006801:Humans; D009154:Mutation; D011829:Radiation Dosage; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000528:radiation effects; D012867:Skin / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects*; D013473:Sunscreening Agents / Q000494:pharmacology; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D013473:Sunscreening Agents; D004247:DNA