title: Differential tapasin dependence of MHC class I molecules correlates with conformational changes upon peptide dissociation: a molecular dynamics simulation study.
abstract: Efficiency of peptide loading to MHC class I molecules in the endoplasmic reticulum is allele specific and can involve interaction with tapasin and other proteins. Allele HLA-B 4,402 depends on tapasin whereas HLA-B 4,405 (Tyr116 instead of Asp in B 4,402) can efficiently load peptides without tapasin. Both alleles adopt very similar structures in the presence of the same peptide. Molecular dynamics simulations on peptide termini dissociation from the alpha(1)/alpha(2) binding domains were used to characterize structural and free energy changes. The magnitude of the calculated free energy change and the shape of the free energy curve vs. distance for induced peptide C terminus dissociation differed for B 4,405 compared to B 4,402. Structural changes during C terminus dissociation occurred mainly in the first segment of the alpha(2)-helix that flanks the peptide C terminus binding region (F pocket) and contacts residue 116. This segment is also close to the proposed tapasin contact region. For B 4402, a stable shift towards an altered open F pocket structure deviating significantly from the bound form was observed. In contrast, B 4405 showed only a transient opening of the F pocket followed by relaxation towards a structure close to the bound (receptive) form upon C terminus dissociation. The greater tendency for a peptide-receptive conformation in the absence of peptide combined with more long-range interactions with the peptide C terminus facilitates peptide binding to B 4405 and correlates with the tapasin-independence of this allele. A possible role of tapasin in case of HLA-B 4402 and other tapasin-dependent alleles could be the stabilization of a peptide-receptive class I conformation.
authors.affiliation: Jacobs University Bremen, Campus Ring 6, 28759 Bremen, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Sieker
authors.forename: Tjerk P
authors.initials: TP
authors.lastname: Straatsma
authors.forename: Sebastian
authors.initials: S
authors.lastname: Springer
authors.forename: Martin
authors.initials: M
authors.lastname: Zacharias
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites; D003198:Computer Simulation; D015395:Histocompatibility Antigens Class I / Q000737:chemistry*; D006860:Hydrogen Bonding; D026901:Membrane Transport Proteins / Q000737:chemistry* / Q000378:metabolism; D008956:Models, Chemical; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D011487:Protein Conformation; D017433:Protein Structure, Secondary; D017434:Protein Structure, Tertiary
chemical_list: D015395:Histocompatibility Antigens Class I; D026901:Membrane Transport Proteins; D010455:Peptides; C107762:tapasin