Facile synthesis of C8-functionalized magnetic silica microspheres for enrichment of low-concentration peptides for direct MALDI-TOF MS analysis.
In this study, novel C8-functionalized magnetic polymer microspheres were prepared by coating single submicron-sized magnetite particle with silica and subsequent modification with chloro (dimethyl) octylsilane. The resulting C8-functionalized magnetic silica (C8-f-M-S) microspheres exhibit well-defined magnetite-core-silica-shell structure and possess high content of magnetite, which endow them with high dispersibility and strong magnetic response. With their magnetic property, the synthesized C8-f-M-S microspheres provide a convenient and efficient way for enrichment of low-abundance peptides from tryptic protein digest and human serum. The enriched peptides/proteins were subjected for MALDI-TOF MS analysis and the enrichment efficiency was documented. In a word, the facile synthesis and efficient enrichment process of the novel C8-f-M-S microspheres make them promising candidates for isolation of peptides even in complex biological samples such as serum, plasma, and urine.
Chen
Hemei
H
Department of Chemistry, Fudan University, Shanghai, China.
Xu
Xiuqing
X
Yao
Ning
N
Deng
Chunhui
C
Yang
Pengyuan
P
Zhang
Xiangmin
X
D002244:Carbon; D052203:Ferrosoferric Oxide; D006801:Humans; D008280:Magnetics; D008863:Microspheres; D010449:Peptide Mapping / Q000295:instrumentation / Q000379:methods*; D010455:Peptides / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification / Q000652:urine; D012822:Silicon Dioxide; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D017550:Spectroscopy, Fourier Transform Infrared
D010455:Peptides; D002244:Carbon; D012822:Silicon Dioxide; D052203:Ferrosoferric Oxide