title: Outbreak of rotavirus variant P[8] in Seoul, South Korea.
abstract: An epidemiologic study was performed to determine the genetic variability of rotaviruses in Seoul, South Korea. In 3,174 stool specimens from children with acute diarrhea at five referral hospitals, 571 (18%) possessed the antigen of group A rotavirus detectable by ELISA--10.8% in 2004 and 28.1% in 2005. VP7 genotyping revealed that the G3 type was found in 25.6% of all typed isolates, G4 in 23.8%, G2 in 21.6%, and G1 in 17.6%. VP4 genotyping showed that the P[8] type was detected in 66.7%, P[6] in 15.6%, P[4] in 13.0%, and P[9] in 0.2%. Because the variant P[8] type could not be amplified initially by conventional P typing primers (1T-1), PCR were performed using newly designed 1T-1S primer, which revealed that 307 specimens were the variant P[8] type. Uncommon combinations such as G4P[6] and G2P[8] were also found with relatively high prevalence, 14.6% and 12.8%, respectively. Variant P[8] types were associated with an outbreak of rotavirus in 2005.
authors.affiliation: Seoul Metropolitan Government Research Institute of Public Health & Environment, Seoul, Korea. shaoma@seoul.go.kr
authors.forename: Jae-In
authors.initials: JI
authors.lastname: Lee
authors.forename: Mi-Ok
authors.initials: MO
authors.lastname: Song
authors.forename: Ju-Young
authors.initials: JY
authors.lastname: Chung
authors.forename: Tae-Hee
authors.initials: TH
authors.lastname: Han
authors.forename: Young-Min
authors.initials: YM
authors.lastname: Ahn
authors.forename: Jeong-Wan
authors.initials: JW
authors.lastname: Seo
authors.forename: Moo-Sang
authors.initials: MS
authors.lastname: Kim
authors.forename: Min-Young
authors.initials: MY
authors.lastname: Kim
authors.forename: Won-Yong
authors.initials: WY
authors.lastname: Kim
authors.forename: Chan-Hee
authors.initials: CH
authors.lastname: Lee
mesh_terms: D000956:Antigens, Viral / Q000032:analysis; D002648:Child; D002675:Child, Preschool; D017931:DNA Primers / Q000235:genetics; D004196:Disease Outbreaks; D004797:Enzyme-Linked Immunosorbent Assay; D005243:Feces / Q000821:virology; D005838:Genotype; D006801:Humans; D007223:Infant; D007723:Korea / Q000453:epidemiology; D016133:Polymerase Chain Reaction / Q000379:methods; D015995:Prevalence; D012367:RNA, Viral / Q000235:genetics; D012401:Rotavirus / Q000145:classification* / Q000302:isolation & purification*; D012400:Rotavirus Infections / Q000453:epidemiology* / Q000821:virology*
chemical_list: D000956:Antigens, Viral; D017931:DNA Primers; D012367:RNA, Viral