Protein expression pattern of murine macrophages treated with anthrax lethal toxin.
Anthrax lethal toxin (LeTx; a combination of protective antigen and lethal factor) secreted by the vegetative cells of Bacillus anthracis is cytotoxic for certain macrophage cell lines. The role of LeTx in mediating these effects is complicated largely due to the difficulty in identifying and assigning functions to the affected proteins. To analyze the protein profile of murine macrophages treated with LeTx, we employed two-dimensional polyacrylamide gel electrophoresis and MALDI-TOF MS, and interpreted the peptide mass fingerprint data relying on the ProFound database. Among the differentially expressed spots, cleaved mitogen-activated protein kinase kinase 1 acting as a negative element in the signal transduction pathway, and glucose-6-phosphate dehydrogenase playing a role in the protection of cells from hyperproduction of active oxygen were up-regulated in the LeTx-treated macrophages.
Jung
Kyoung Hwa
KH
Division of Molecular and Life Sciences, Hanyang University, Ansan, Gyeonggi-do, 426-791, Republic of Korea.
Seo
Gwi Moon
GM
Yoon
Jang Won
JW
Park
Kyoung Sun
KS
Kim
Ji Cheon
JC
Kim
Seong Joo
SJ
Oh
Kwang Gun
KG
Lee
Jung-Ho
JH
Chai
Young Gyu
YG
D000818:Animals; D000942:Antigens, Bacterial / Q000494:pharmacology*; D001427:Bacterial Toxins / Q000494:pharmacology*; D002478:Cells, Cultured; D015180:Electrophoresis, Gel, Two-Dimensional; D008264:Macrophages / Q000187:drug effects* / Q000378:metabolism*; D051379:Mice; D020929:Mitogen-Activated Protein Kinase Kinases / Q000032:analysis / Q000378:metabolism; D020543:Proteome / Q000032:analysis / Q000187:drug effects*
D000942:Antigens, Bacterial; D001427:Bacterial Toxins; D020543:Proteome; C030325:anthrax toxin; D020929:Mitogen-Activated Protein Kinase Kinases