title: Simultaneously subtyping of all influenza A viruses using DNA microarrays.
abstract: Rapid diagnosis of novel emerging subtypes of influenza viruses is vital for effective global influenza surveillance. To this end, a novel microarray based surveillance was developed for subtyping all influenza A viruses on one chip. Using reference strains of different influenza subtypes and samples from different areas, the results show that all the subtypes of the influenza A virus could be identified simultaneously on this microchip with high sensitivity. There was no cross-hybridization reaction with other viruses, indicating that the microarray is specific for influenza A viruses. Such a diagnostic microarray will undoubtedly be useful for identifying novel influenza A virus subtypes.
authors.affiliation: Chinese Academy of Inspection and Quarantine, Beijing 100029, China. hanxueq@yahoo.com.cn
authors.forename: Xueqing
authors.initials: X
authors.lastname: Han
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Lin
authors.forename: Bohua
authors.initials: B
authors.lastname: Liu
authors.forename: Yihong
authors.initials: Y
authors.lastname: Hou
authors.forename: Jinhai
authors.initials: J
authors.lastname: Huang
authors.forename: Shaoqiang
authors.initials: S
authors.lastname: Wu
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.forename: Lin
authors.initials: L
authors.lastname: Mei
authors.forename: Guangle
authors.initials: G
authors.lastname: Jia
authors.forename: Qingyu
authors.initials: Q
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001717:Birds; D004279:DNA, Viral / Q000235:genetics; D016679:Genome, Viral; D006801:Humans; D009980:Influenza A virus / Q000145:classification* / Q000235:genetics*; D005585:Influenza in Birds / Q000821:virology; D007251:Influenza, Human / Q000821:virology; D020411:Oligonucleotide Array Sequence Analysis / Q000295:instrumentation / Q000379:methods*; D012367:RNA, Viral / Q000235:genetics; D012680:Sensitivity and Specificity
chemical_list: D004279:DNA, Viral; D012367:RNA, Viral