Structural characterization of a water-soluble polysaccharide from the roots of Codonopsis pilosula and its immunity activity.
The water-soluble polysaccharide (CPP), with a molecular mass of 1.1x10(4) Da, was obtained from the roots of Codonopsis pilosula. Structure feature investigation by a combination of chemical and instrumental analysis revealed that CPP had a backbone consisting of (1-->3)-linked-beta-D-galactopyranosyl, (1-->2, 3)-linked-beta-D-galactopyranosyl and (1-->3)-linked-alpha-D-rhamnopyranosyl residues, which were branched with two glycosyl residues composed of alpha-L-arabinose-(1-->5)-alpha-L-arabinose(1-->linked residues at the O-2 position of galactosyl along the main chain in the ratio of 1:1:2:1:1. Preliminary immunological tests in vitro showed CPP could stimulate concanavalin A (ConA)- or lipopolysaccharide (LPS)-induced lymphocyte proliferation in a dose-dependent manner.
Yongxu
Sun
S
Department of Pharmacology, Qiqihar Medical University, Qiqihar 161042, PR China. yongxusun1978@yahoo.com.cn
Jicheng
Liu
L
D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D029745:Codonopsis / Q000737:chemistry*; D003208:Concanavalin A / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D008070:Lipopolysaccharides / Q000737:chemistry / Q000276:immunology / Q000302:isolation & purification / Q000494:pharmacology; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects; D008297:Male; D051379:Mice; D018517:Plant Roots / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology* / Q000302:isolation & purification / Q000494:pharmacology; D012995:Solubility; D014867:Water / Q000737:chemistry*
D008070:Lipopolysaccharides; D011134:Polysaccharides; D014867:Water; D003208:Concanavalin A