Optimization of bulk cell electrofusion in vitro for production of human-mouse heterohybridoma cells.
Cell electrofusion is a phenomenon that occurs, when cells are in close contact and exposed to short high-voltage electric pulses. The consequence of exposure to pulses is transient and nonselective permeabilization of cell membranes. Cell electrofusion and permeabilization depend on the values of electric field parameters including amplitude, duration and number of electric pulses and direction of the electric field. In our study, we first investigated the influence of the direction of the electric field on cell fusion in two cell lines. In both cell lines, applications of pulses in two directions perpendicular to each other were the most successful. Cell electrofusion was finally used for production of human-mouse heterohybridoma cells with modified Koehler and Milstein hybridoma technology, which was not done previously. The results, obtained by cell electrofusion, are comparable to usually used polyethylene glycol mediated fusion on the same type of cells.
Trontelj
Katja
K
University of Ljubljana, Faculty of Electrical Engineering, Trzaska 25, SI-1000 Ljubljana, Slovenia.
Rebersek
Matej
M
Kanduser
Masa
M
Serbec
Vladka Curin
VC
Sprohar
Marjana
M
Miklavcic
Damijan
D
D000818:Animals; D002459:Cell Fusion / Q000379:methods*; D018274:Electroporation / Q000379:methods*; D006801:Humans; D006825:Hybridomas / Q000166:cytology*; D051379:Mice