title: Techniques developed in China for foot-and-mouth disease diagnosis.
abstract: Over the past several decades, a series of new methods have been developed in China for the diagnosis of foot-and-mouth disease (FMD). These methods are theoretically consistent with Office International des Epizooties standards for FMD diagnosis and include: antigen-capture ELISA and colloid-gold test strip for viral antigen typing, liquid-phase blocking ELISA (LPBE) for detection of antibodies against O and Asia 1 FMDV, and an indirect ELISA for detection of antibodies against the non-structural protein (NSP) 3ABC (3ABC-I-ELISA). Several molecular diagnostic methods have also been developed for detection of fragments of the FMDV genome within viral samples, such as multiplex RT-PCR, typing RT-PCR and real-time RT-PCR. In China, FMD vaccines are compulsorily used in all livestock. In such a situation, monitoring of the immune status of animal herds and disease surveillance become very important. Liquid-phase blocking ELISA is routinely used in China for monitoring the immune status, and 3ABC-I-ELISA is feasible for large-scale serological surveys based on differentiation of FMDV-infected animals from vaccinated animals. Combined use of 3ABC-ELISA and mRT-PCR is described for survey of FMD virus-prevalent herds and virus carriers.
authors.affiliation: State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agriculture Science, Xujiaping No 1, Yanchangpu, Lanzhou, Gansu 730046, China. liukey@public.lz.gs.cn
authors.forename: Z
authors.initials: Z
authors.lastname: Lu
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.forename: H
authors.initials: H
authors.lastname: Bao
authors.forename: S
authors.initials: S
authors.lastname: Qi
authors.forename: J
authors.initials: J
authors.lastname: Guo
authors.forename: Y
authors.initials: Y
authors.lastname: Shang
authors.forename: T
authors.initials: T
authors.lastname: Jiang
authors.forename: Q
authors.initials: Q
authors.lastname: Zhang
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.forename: Z
authors.initials: Z
authors.lastname: Liu
authors.forename: X
authors.initials: X
authors.lastname: Liu
authors.forename: H
authors.initials: H
authors.lastname: Yin
authors.forename: Q
authors.initials: Q
authors.lastname: Xie
mesh_terms: D000818:Animals; D002353:Carrier State / Q000662:veterinary; D002681:China; D019411:Clinical Laboratory Techniques / Q000592:standards / Q000662:veterinary*; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods / Q000662:veterinary; D005536:Foot-and-Mouth Disease / Q000175:diagnosis* / Q000517:prevention & control; D029544:Foot-and-Mouth Disease Virus / Q000302:isolation & purification*; D016133:Polymerase Chain Reaction / Q000379:methods / Q000662:veterinary; D018571:Sentinel Surveillance / Q000662:veterinary; D014765:Viral Vaccines / Q000008:administration & dosage*
chemical_list: D014765:Viral Vaccines