[Biotransformation of podophyllotoxin by cell suspension culture and root culture of Rheum palmatum].
OBJECTIVE
To study the biotransformation of podophyllotoxin by the cell suspension culture and root culture systems of Rheum palmatum.


METHODS
Using plant tissue culture technology and HPLC techniques to isolate products. The structures were elucidated by spectroscopic means.


RESULTS
Cell suspension culture of R. palmatum could convert podophyllotoxin to produce picropodophyllotoxin with the yield of 73.8%, while root culture of R. palmatum could convert podophyllotoxin to produce epipodophyllotoxin and apopodophyllotoxin.


CONCLUSIONS
Podophyllotoxin did not affect the pH value of the media used in tissue cultures. Both cell suspension culture and root culture of R. palmatum can convert podophyllotoxin.
Cui
Ya-Jun
YJ
School of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Liu
Xiao-Feng
XF
Han
Jian
J
Wang
Bao-Rong
BR
Guo
De-An
DA
D002851:Chromatography, High Pressure Liquid; D015394:Molecular Structure; D018517:Plant Roots / Q000378:metabolism*; D011034:Podophyllotoxin / Q000737:chemistry / Q000378:metabolism*; D012250:Rheum / Q000378:metabolism*; D046509:Tissue Culture Techniques / Q000379:methods*
D011034:Podophyllotoxin