title: [Different change of olive tail moment and cytokinesis-block micronucleus frequency of the cells of 4 kinds treated with polycyclic aromatic hydrocarbon].
abstract: OBJECTIVE
  To investigate the difference change of olive tail moment (TM) and cytokinesis-block micronucleus (CBMN) frequency of the cells of four kinds treated with polycyclic aromatic hydrocarbon.
  
  
  METHODS
  Four different cell lines, CHO-K1, NIH3T3, CHL, and HELF were treated with B(a)P in vitro, and the genetic damage were detected by Comet and CBMN assay, respectively. The positive controls were Hydro peroxides (H2O2 ) and Cyclohosphamide (CP) for Comet and CBMN assay, respectively.
  
  
  RESULTS
  The changes of olive TM levels of four cell lines treated with B(a)P were occurred at the lower doses of B(a)P (CHO - K1:0.5 micromol/L, NIH3T3 : 0.75 micromol/L, CHL : 0.5 micromol/L, HELF : 0.3125 micromol/L) than that of CBMN frequencies (CHO-K1 : 2.0 micromol/L, NIH3T3 : 1.51 micromol/L, CHL : 2.0 micromol/L, HELF : 1.25 micromol/L). The dose-response curve of B(a)P was linear for Olive TM, but sigmoid for CBMN frequency.
  
  
  CONCLUSIONS
  The data showed that olive TM was more sensitive than CBMN frequency for the genotoxic detects of B(a)P for the four cell lines.
authors.affiliation: National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Cheng
authors.forename: Shuguang
authors.initials: S
authors.lastname: Leng
authors.forename: Yong
authors.initials: Y
authors.lastname: Niu
authors.forename: Huawei
authors.initials: H
authors.lastname: Duan
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D016466:CHO Cells; D002460:Cell Line; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D048749:Cytokinesis / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008168:Lung / Q000166:cytology; D051379:Mice; D015162:Micronucleus Tests; D041681:NIH 3T3 Cells; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*
chemical_list: D011084:Polycyclic Aromatic Hydrocarbons; D001564:Benzo(a)pyrene