Solid emulsion gel as a vehicle for delivery of polyunsaturated fatty acids: implications for tissue repair, dermal angiogenesis and wound healing.
The paper describes preparation and biological characterization of the solid hybrid biomaterial that was designed for cell-targeted lipid delivery in healing tissues. The material referred to as 'solid emulsion gel' combines a protein-stabilized lipid emulsion and a hydrogel structure in a single compartment. The potential of the omega-3 (n-3)-fatty acids rich solid emulsion gel for tissue repair applications was investigated at the macro-, micro-, molecular and gene expression levels, using human fibroblasts and endothelial cells and a porcine model of full-thickness wounds. Being non-cytotoxic in vitro and in vivo, the biomaterial was found to affect cell metabolism, modulate expression of certain genes, stimulate early angiogenesis and promote wound repair in vivo. The neovascular response in vivo was correlated with upregulated expression of the genes involved in lipid transport (e.g. adipophilin), anti-apoptosis (e.g. heat shock proteins, haem oxygenase 1) and angiogenesis (vascular endothelial growth factor, placental growth factor). Collectively, the results of this study provide first evidence that the angiogenic response provided by solid emulsion gel-mediated delivery of n-3 fatty acids is an alternative to the topical administration of exogenous growth factors or gene therapy, and can be advantageously used for the stimulation of tissue repair in complex wounds.
Shingel
Kirill I
KI
BioArtificial Gel Technologies (BAGTECH) Inc., 400 Maisonneuve Ouest, Suite 1156, Montreal, Quebec, Canada H3A 1L4. kirill.shingel@bagtech.com
Faure
Marie-Pierre
MP
Azoulay
Laurent
L
Roberge
Christophe
C
Deckelbaum
Richard J
RJ
D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D020405:Dermis / Q000293:injuries* / Q000473:pathology; D004655:Emulsions / Q000737:chemistry*; D004730:Endothelium, Vascular / Q000166:cytology; D015525:Fatty Acids, Omega-3 / Q000378:metabolism; D005231:Fatty Acids, Unsaturated / Q000737:chemistry*; D005782:Gels / Q000737:chemistry*; D020869:Gene Expression Profiling; D006801:Humans; D008954:Models, Biological; D009389:Neovascularization, Pathologic; D044968:Regenerative Medicine / Q000379:methods*; D013552:Swine; D014945:Wound Healing
D001672:Biocompatible Materials; D004655:Emulsions; D015525:Fatty Acids, Omega-3; D005231:Fatty Acids, Unsaturated; D005782:Gels