Expression of a synthetic Artemesia annua amorphadiene synthase in Aspergillus nidulans yields altered product distribution.
A gene encoding a plant terpene cyclase, Artemisia annua amorpha-4,11-diene synthase (ADS), was expressed in Aspergillus nidulans under control of a strong constitutive promoter, (p)gpdA. The transformants produced only small amounts of amorphadiene, but much larger amounts of similar sesquiterpenes normally produced as minor by-products in planta. In contrast, expression of ADS in Escherichia coli produced almost exclusively amorpha-4,11-diene. These results indicate that the host environment can greatly impact the terpenes produced from terpene synthases.
Lubertozzi
David
D
Department of Chemical Engineering, University of California, Berkeley, CA 94720, USA.
Keasling
Jay D
JD
D019883:Alkyl and Aryl Transferases / Q000235:genetics* / Q000378:metabolism; D031172:Artemisia annua / Q000201:enzymology*; D001233:Aspergillus nidulans / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D005813:Genes, Synthetic; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism
D010940:Plant Proteins; D013729:Terpenes; D019883:Alkyl and Aryl Transferases; C108877:terpene synthase