title: Use of tissue ink to maintain identification of individual cores on needle biopsies of the prostate.
abstract: BACKGROUND
  There is an increasing necessity to extract the maximum amount of information, beyond even a cancer diagnosis, from prostate biopsies. Thus, maintaining site-specific information regarding individual biopsy cores might be critical.
  
  
  OBJECTIVE
  To evaluate the applicability of employing tissue ink to maintain the identity of individual prostatic biopsy cores.
  
  
  METHODS
  In this ongoing study, 12 core prostate biopsy specimens are sent to the laboratory in individual pots labelled according to anatomical site. The specimens are placed in two separate multi-compartment cassettes. They are inked with different colours to identify the site of origin from each lobe. The cassettes are then processed with a single paraffin block for each side; the six cores from each side can be mounted on a single slide.
  
  
  RESULTS
  The different colours used adhere well to the biopsy cores, thus maintaining the identity of each core. Six cores from each side are embedded in a single paraffin block and examined on a single slide, making it cost-effective, while maintaining high quality, accurate histopathological information.
  
  
  CONCLUSIONS
  Differential inking of prostate biopsy cores is an easily applicable method that is cost-effective and provides tumour location information. Prostate biopsy data archived to maintain individual core information might be used to determine applicability of such information to predict extra-capsular extension by correlating with imaging and radical prostatectomy findings, and for treatment planning.
authors.affiliation: Lancashire Teaching Hospitals NHS Foundation Trust, Fulwood, Preston, UK. parassingh@btinternet.com
authors.forename: P B
authors.initials: PB
authors.lastname: Singh
authors.forename: N K
authors.initials: NK
authors.lastname: Saw
authors.forename: A
authors.initials: A
authors.lastname: Haq
authors.forename: R A
authors.initials: RA
authors.lastname: Blades
authors.forename: F L
authors.initials: FL
authors.lastname: Martin
authors.forename: S S
authors.initials: SS
authors.lastname: Matanhelia
authors.forename: C M
authors.initials: CM
authors.lastname: Nicholson
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D001707:Biopsy, Needle / Q000379:methods*; D004396:Coloring Agents; D006801:Humans; D007150:Immunohistochemistry; D007281:Ink; D008297:Male; D008875:Middle Aged; D011467:Prostate / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology*; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D004396:Coloring Agents