Plaques from different individuals yield different microbiota responses to oral-antiseptic treatment.
Dental caries is a polymicrobial disease and complicated to treat. Understanding the microbiota responses to treatment from different individuals is a key factor in developing effective treatments. The aim of this study was to investigate the 24-h posttreatment effect of two oral antiseptics (chlorhexidine and Listerine) on species composition of microplate plaque biofilms that had been initiated from the saliva of five different donors and grown in both 0.15% and 0.5% sucrose. Plaque composition was analyzed using checkerboard DNA : DNA hybridization analysis, which comprised of a panel of 40 species associated with oral health and disease. The supernatant pH of the plaques grown in 0.15% sucrose ranged from 4.3 to 6 and in 0.5% sucrose, it ranged from 3.8 to 4. Plaque biomass was largely unaffected by either antiseptic. Each donor had a different salivary microbial profile, differentiating according to the prevalence of either caries or periodontal/anaerobic pathogens. Despite similar plaque microbiota compositions being elicited through the sucrose growth conditions, microbiota responses to chlorhexidine and Listerine differentiated according to the donor. These findings indicate that efficacious caries treatments would depend on the responses of an individual's microbiota, which may differ from person to person.
Filoche
Sara K
SK
Dental Research Group, Department of Pathology and Molecular Medicine, Wellington School of Medicine and Health Sciences, University of Otago, Wellington, New Zealand. sara.filoche@otago.ac.nz
Soma
Dennes
D
van Bekkum
Margo
M
Sissons
Chris H
CH
D000891:Anti-Infective Agents, Local / Q000494:pharmacology*; D001420:Bacteria, Aerobic / Q000145:classification / Q000187:drug effects / Q000235:genetics; D001421:Bacteria, Anaerobic / Q000145:classification / Q000187:drug effects / Q000235:genetics; D018441:Biofilms / Q000254:growth & development; D018533:Biomass; D002710:Chlorhexidine / Q000494:pharmacology*; D003731:Dental Caries / Q000382:microbiology; D003773:Dental Plaque / Q000188:drug therapy* / Q000382:microbiology*; D004338:Drug Combinations; D017753:Ecosystem; D006801:Humans; D009067:Mouthwashes / Q000494:pharmacology*; D009693:Nucleic Acid Hybridization; D010518:Periodontitis / Q000382:microbiology; D012459:Salicylates / Q000494:pharmacology*; D012463:Saliva / Q000382:microbiology; D013729:Terpenes / Q000494:pharmacology*; D016896:Treatment Outcome
D000891:Anti-Infective Agents, Local; D004338:Drug Combinations; D009067:Mouthwashes; D012459:Salicylates; D013729:Terpenes; C057473:Listerine; D002710:Chlorhexidine