title: Use of GRA6-derived synthetic polymorphic peptides in an immunoenzymatic assay to serotype Toxoplasma gondii in human serum samples collected from three continents.
abstract: Serotyping is a simple typing method that consists of an immunoenzymatic assay (enzyme-linked immunosorbent assay [ELISA]) using synthetic polymorphic peptides derived from Toxoplasma gondii antigens. We developed a new ELISA based on GRA6 C-terminal polymorphic peptides. Serum samples from 41 human infections due to 23 archetypal (type I, II, or III) and 18 nonarchetypal strains were selected in order to validate this approach. For 20 out of the 23 archetypal infections, there was a clear correlation between microsatellite genotype and GRA6 serotyping. All infections due to nonarchetypal strains were misclassified as archetypal strain infections. The GRA6 C-terminal peptides from these strains were analyzed to explain this misclassification. A second group of 455 patients with acute and chronic toxoplasmosis due to unknown genotypes from different European, African, and Latin American countries were included in this study, and the strain type predicted by this method. The results suggest that serotyping is a promising method for typing strains, although limitations exist for African and South American strains as a consequence of higher peptide polymorphism. Other peptides from different markers must be studied in order to discriminate archetypal from nonarchetypal strains.
authors.affiliation: Center of Parasite Immunology and Biology, INSA, Rua de S. Luis 16, 4000-509 Porto, Portugal.
authors.forename: Susana
authors.initials: S
authors.lastname: Sousa
authors.forename: Daniel
authors.initials: D
authors.lastname: Ajzenberg
authors.forename: Manuel
authors.initials: M
authors.lastname: Vilanova
authors.forename: José
authors.initials: J
authors.lastname: Costa
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Dardé
mesh_terms: D000349:Africa; D000595:Amino Acid Sequence; D000818:Animals; D000953:Antigens, Protozoan; D016054:DNA, Protozoan / Q000235:genetics; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods; D005060:Europe; D006801:Humans; D007843:Latin America; D018895:Microsatellite Repeats; D008969:Molecular Sequence Data; D015800:Protozoan Proteins; D012703:Serotyping / Q000379:methods; D013020:South America; D013223:Statistics as Topic; D014122:Toxoplasma / Q000145:classification* / Q000235:genetics / Q000276:immunology / Q000302:isolation & purification*; D014123:Toxoplasmosis / Q000469:parasitology*
chemical_list: D000953:Antigens, Protozoan; D016054:DNA, Protozoan; C095047:GRA6 protein, Toxoplasma gondii; D015800:Protozoan Proteins