[Effect of puerarin on PC12 cells apoptosis induced by Abeta25-35 in vitro].
OBJECTIVE
To establish a nerve cell injury model by incubating PC12 cell line in the presence of Abeta25-35 to study the effect of puerarin on apoptosis of nerve cells.


METHODS
PC12 cells were incubated with Abeta25-35 and puerarin. Cell viability was detected by MTT. The cellular morphology was observed with electron microscopy. FITC-labeled Annexin V and propidium iodide were adopted to evaluate the rate of cell apoptosis in different groups by means of flow cytometry.


RESULTS
Following incubation Abeta25-35, the cells were induced to undergo apoptosis. The viability of PC12 cells decreased in a time-dependent manner. Morphological evidences for apoptosis nuclear condensation were observed in PC12 cells. Cells incubated in the presence of Abeta25-35 showed increasing apoptotic rates, but cells treated with puerarin and Abeta25-35 revealed decreasing apoptotic rates, it demonstrated that puerarin had a significant protective action against Abeta25-35 evoked apoptosis.


CONCLUSIONS
Puerarin can resist the adverse effects of Abeta25-35 on increasing apoptotic rates, and it has the protective function towards nerve cells.
Zhang
Hai-Ying
HY
Department of Human Anatomy and Histology Embryology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China. zhanghai_ying@sina.com
Hu
Hai-Tao
HT
Liu
Yi-Heng
YH
Wang
Hong-Quan
HQ
Feng
Gai-Feng
GF
Chen
Guo-Min
GM
D000544:Alzheimer Disease / Q000473:pathology*; D016229:Amyloid beta-Peptides; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D007887:Fabaceae / Q000737:chemistry; D005434:Flow Cytometry; D007529:Isoflavones / Q000494:pharmacology*; D018696:Neuroprotective Agents / Q000494:pharmacology*; D016716:PC12 Cells; D010446:Peptide Fragments; D051381:Rats; D014665:Vasodilator Agents / Q000494:pharmacology
D016229:Amyloid beta-Peptides; D007529:Isoflavones; D018696:Neuroprotective Agents; D010446:Peptide Fragments; D014665:Vasodilator Agents; C074636:amyloid beta-protein (25-35); C033607:puerarin