title: Enhancement of heterologous production of eicosapentaenoic acid in Escherichia coli by substitution of promoter sequences within the biosynthesis gene cluster.
abstract: To enhance the heterologous production of eicosapentaenoic acid (EPA) in Escherichia coli, the EPA biosynthesis gene cluster from Shewanella oneidensis MR-1 was cloned under the lacZ promoter on a high-copy number plasmid, pBluescript SK+. The production of EPA was remarkably enhanced yielding levels of up to 7.5% of the total fatty acid content in the recombinant E. coli strain by induction with IPTG, whereas the stimulation of EPA production was abolished by adding glucose into the culture medium, probably due to glucose repression acting on the promoter activity.
authors.affiliation: Molecular Bioprocess Research Center, Jeonbuk Branch Institute, KRIBB, Jeongeup, Jeonbuk 580-185, South Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Lee
authors.forename: Chul Ho
authors.initials: CH
authors.lastname: Kim
authors.forename: Pil-Soo
authors.initials: PS
authors.lastname: Seo
authors.forename: Ohsuk
authors.initials: O
authors.lastname: Kwon
authors.forename: Byung-Ki
authors.initials: BK
authors.lastname: Hur
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Seo
mesh_terms: D003001:Cloning, Molecular; D015118:Eicosapentaenoic Acid / Q000096:biosynthesis* / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D015870:Gene Expression; D005798:Genes, Bacterial; D007763:Lac Operon; D005810:Multigene Family; D016133:Polymerase Chain Reaction; D011401:Promoter Regions, Genetic; D020592:Shewanella / Q000235:genetics* / Q000378:metabolism; D017855:Transferases (Other Substituted Phosphate Groups) / Q000235:genetics
chemical_list: D015118:Eicosapentaenoic Acid; D017855:Transferases (Other Substituted Phosphate Groups); C021364:holo-(acyl-carrier-protein) synthase