title: [Preparation of chitosan microparticles and study of their interaction with interferon].
abstract: Chitosan with viscosity average molecular weights of 340, 281, 199, 137, and 42 kDa was used to prepare microparticles by precipitation coacervation. Acid and enzymatic hydrolysis yielded chitosan samples with a deacetylation degree of 0.85 +/- 0.03. The chitosan microparticles were 0.85-1.7 microm in size and had a zeta-potential of 30.7-38 +/- 0.1 mV. Testing the microparticles for toxicity showed that they caused no death of animals. Interaction of recombinant alpha-2 interferon with the microparticles was studied by sorption from solutions. The maximum adsorption efficiency of interferon was 88% and the capacity of microparticles was 11.8-12.7 microg/mg.
authors.forename: A V
authors.initials: AV
authors.lastname: Il'ina
authors.forename: A A
authors.initials: AA
authors.lastname: Gubaĭdullina
authors.forename: A I
authors.initials: AI
authors.lastname: Melent'ev
authors.forename: V P
authors.initials: VP
authors.lastname: Varlamov
mesh_terms: D000327:Adsorption; D000818:Animals; D048271:Chitosan / Q000737:chemistry* / Q000633:toxicity; D016503:Drug Delivery Systems; D007370:Interferon Type I / Q000008:administration & dosage / Q000737:chemistry*; D008297:Male; D051379:Mice; D008863:Microspheres; D010316:Particle Size; D011994:Recombinant Proteins
chemical_list: D007370:Interferon Type I; D011994:Recombinant Proteins; D048271:Chitosan