Differences in immunoreactivities of Ki-67 and doublecortin in the adult hippocampus in three strains of mice.
Neurogenesis in the adult hippocampus is differentially influenced by the genetic background. We examined the differences in Ki-67 (a proliferating cell marker) and doublecortin (DCX; an immature progenitor cell marker) immunolabelling in the dentate gyrus (DG) of the adult hippocampus in three strains of mice (ICR, C57BL/6, and BALB/c) to evaluate the effect of genetic background on adult hippocampal neurogenesis. All strains showed constitutive immunoreactivity of either Ki-67 or DCX in the DG of the adult hippocampus. C57BL/6 mice showed significantly higher levels of Ki-67-immunopositive cells in the subgranular zone (SGZ) of the DG (approximately 2.2-fold) compared to ICR and BALB/c mice. The greatest number of DCX-immunopositive cells was found in C57BL/6 (approximately 1.6-fold), which differed significantly from ICR and BALB/c mice. However, there was no significant difference in the number of Ki-67- and DCX-immunopositive cells between BALB/c and ICR mice. Genetic differences with respect to certain aspects of hippocampal neurogenesis in adult mice may influence hippocampal functions, including learning and memory.
Kim
Joong-Sun
JS
Department of Veterinary Anatomy, College of Veterinary Medicine and Veterinary Medical Research Center, Chonnam National University, Gwangju, South Korea.
Jung
Jisun
J
Lee
Hae-June
HJ
Kim
Jong Choon
JC
Wang
Hongbing
H
Kim
Sung-Ho
SH
Shin
Taekyun
T
Moon
Changjong
C
D000704:Analysis of Variance; D000818:Animals; D002452:Cell Count / Q000379:methods; D018891:Dentate Gyrus / Q000166:cytology* / Q000254:growth & development; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D006624:Hippocampus / Q000166:cytology* / Q000254:growth & development; D007150:Immunohistochemistry; D019394:Ki-67 Antigen / Q000276:immunology / Q000378:metabolism*; D008297:Male; D051379:Mice / Q000502:physiology; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D008813:Mice, Inbred ICR; D008869:Microtubule-Associated Proteins / Q000276:immunology / Q000378:metabolism*; D055495:Neurogenesis / Q000502:physiology*; D009474:Neurons / Q000378:metabolism* / Q000502:physiology; D009479:Neuropeptides / Q000276:immunology / Q000378:metabolism*; D013045:Species Specificity; D013234:Stem Cells / Q000378:metabolism* / Q000502:physiology
C000714847:Dcx protein, mouse; D000089622:Doublecortin Domain Proteins; D000089662:Doublecortin Protein; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides