Structural insights into P-glycoprotein (ABCB1) by small angle X-ray scattering and electron crystallography.
P-glycoprotein (ABCB1) is an ATP-binding cassette protein that is associated with the acquisition of multi-drug resistance in cancer and the failure of chemotherapy in humans. Structural insights into this protein are described using a combination of small angle X-ray scattering data and cryo-electron crystallography data. We have compared the structures with bacterial homologues, and discuss the development of homology models for P-glycoprotein based on the bacterial Sav1866 structure.
McDevitt
Christopher A
CA
Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
Shintre
Chitra A
CA
Grossmann
J Günter
JG
Pollock
Naomi L
NL
Prince
Stephen M
SM
Callaghan
Richard
R
Ford
Robert C
RC
D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1 / Q000737:chemistry*; D001426:Bacterial Proteins / Q000737:chemistry; D020285:Cryoelectron Microscopy; D003461:Crystallography; D004583:Electrons; D006801:Humans; D011487:Protein Conformation; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
C513055:ABCB1 protein, human; D018435:ATP Binding Cassette Transporter, Subfamily B; D020168:ATP Binding Cassette Transporter, Subfamily B, Member 1; D001426:Bacterial Proteins