[Study on expression of Ras family gene in transformed cells induced by BPDE].
OBJECTIVE
To explore the expression of Ras family gene in the malignantly transformed human bronchial epithelial cell line induced by anti-benzo(a)pyrene-trans-7,8- dihydrodiol-9,10-epoxide(BPDE).


METHODS
The levels of H-Ras, K-Ras and N-Ras mRNA expression in BPDE transformed 16HBE cells (16HBE-T) and untransformed control 16HBE cells (16HBE-N) were examined using RT-PCR. Expression levels of those three proteins in both kinds of cells were analyzed by western blot and immunocytochemical method.


RESULTS
Compared with 16HBE-N, the levels of H-Ras, K-Ras and N-Ras mRNA expression were significantly increased to 2.237, 1.254 and 3.616 times in 16HBE-T, and the protein levels of those genes were increased to 1.273, 1.547 and 1.600 times, respectively. Immunocytochemical method showed expression levels of those proteins in 16HBE-T were significantly higher than those in 16HBE-N.


CONCLUSIONS
H-Ras, K-Ras and N-Ras are overexpressed in 16HBE-T. The activation of oncogenic Ras may participate in malignant transformation of 16HBE induced by BPDE.
Zhou
Lanlan
L
Institute for Chemical Carcinogenesis, Department of Preventive Medicine, Guangzhou Medical College, Guangzhou 510182, China. zhoulanlan19820912@yahoo.com.cn
Tan
Aijun
A
Jiang
Yiguo
Y
Shen
Yuelan
Y
D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / Q000633:toxicity*; D001980:Bronchi / Q000166:cytology; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000139:chemically induced*; D004847:Epithelial Cells / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018631:ras Proteins / Q000235:genetics / Q000378:metabolism*
D012333:RNA, Messenger; D015123:7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide; D018631:ras Proteins