A role for inositol hexakisphosphate in the maintenance of basal resistance to plant pathogens.
Phytic acid (myo-inositol hexakisphosphate, InsP6) is an important phosphate store and signal molecule in plants. However, low-phytate plants are being developed to minimize the negative health effects of dietary InsP6 and pollution caused by undigested InsP6 in animal waste. InsP6 levels were diminished in transgenic potato plants constitutively expressing an antisense gene sequence for myo-inositol 3-phosphate synthase (IPS, catalysing the first step in InsP6 biosynthesis) or Escherichia coli polyphosphate kinase. These plants were less resistant to the avirulent pathogen potato virus Y and the virulent pathogen tobacco mosaic virus (TMV). In Arabidopsis thaliana, mutation of the gene for the enzyme catalysing the final step of InsP6 biosynthesis (InsP5 2-kinase) also diminished InsP6 levels and enhanced susceptibility to TMV and to virulent and avirulent strains of the bacterial pathogen Pseudomonas syringae. Arabidopsis thaliana has three IPS genes (AtIPS1-3). Mutant atips2 plants were depleted in InsP6 and were hypersusceptible to TMV, turnip mosaic virus, cucumber mosaic virus and cauliflower mosaic virus as well as to the fungus Botrytis cinerea and to P. syringae. Mutant atips2 and atipk1 plants were as hypersusceptible to infection as plants unable to accumulate salicylic acid (SA) but their increased susceptibility was not due to reduced levels of SA. In contrast, mutant atips1 plants, which were also depleted in InsP6, were not compromised in resistance to pathogens, suggesting that a specific pool of InsP6 regulates defence against phytopathogens.
Murphy
Alex M
AM
Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK. alex.murphy@plantsci.cam.ac.uk
Otto
Bettina
B
Brearley
Charles A
CA
Carr
John P
JP
Hanke
David E
DE
D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism* / Q000382:microbiology / Q000821:virology; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism; D020171:Botrytis / Q000472:pathogenicity; D017796:Caulimovirus / Q000472:pathogenicity; D017799:Cucumovirus / Q000472:pathogenicity; D004269:DNA, Bacterial / Q000235:genetics; D004198:Disease Susceptibility / Q000382:microbiology / Q000821:virology; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D007113:Immunity, Innate / Q000235:genetics; D016254:Mutagenesis, Insertional; D009154:Mutation; D007296:Myo-Inositol-1-Phosphate Synthase / Q000235:genetics / Q000378:metabolism*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D010833:Phytic Acid / Q000096:biosynthesis*; D010935:Plant Diseases / Q000235:genetics / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism / Q000382:microbiology / Q000821:virology; D017800:Potyvirus / Q000472:pathogenicity; D044224:Pseudomonas syringae / Q000472:pathogenicity; D018749:RNA, Plant / Q000235:genetics; D020156:Salicylic Acid / Q000378:metabolism; D015398:Signal Transduction; D011198:Solanum tuberosum / Q000235:genetics / Q000378:metabolism* / Q000821:virology; D014027:Tobacco Mosaic Virus / Q000472:pathogenicity; D017806:Tymovirus / Q000472:pathogenicity
D029681:Arabidopsis Proteins; D004269:DNA, Bacterial; D010940:Plant Proteins; D018749:RNA, Plant; C036483:T-DNA; D010833:Phytic Acid; D017853:Phosphotransferases (Alcohol Group Acceptor); C508906:inositol 1,3,4,5,6-pentakisphosphate 2-kinase, Arabidopsis; D007296:Myo-Inositol-1-Phosphate Synthase; D020156:Salicylic Acid