title: Sequence analysis of the VP2 hypervariable region of eight very virulent infectious bursal disease virus isolates from the northeast of China.
abstract: Nucleotide sequences of the VP2 gene of eight infectious bursal disease viruses isolated from vaccinated chicken flocks in the northeast of China were determined. The sequence analysis showed that all of the isolates were also characterized by the vvIBDV conserved amino acid residues: 222A, 256I, 294I, and 299S. Four of them had one amino acid change (D-->N) at position 212 in VP2 major hydrophilic peak A, while two of the four isolates had another one (A-->V) at position 321 in major hydrophilic peak B. The other isolates were similar to the UK661 strain. Our findings demonstrated that the vvlBDV strains in the northeast of China could be diverse.
authors.affiliation: Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China.
authors.forename: Yanqing
authors.initials: Y
authors.lastname: Yuwen
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gao
authors.forename: Honglei
authors.initials: H
authors.lastname: Gao
authors.forename: Xiaole
authors.initials: X
authors.lastname: Qi
authors.forename: Tieqiang
authors.initials: T
authors.lastname: Li
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D018175:Birnaviridae Infections / Q000662:veterinary* / Q000821:virology; D002645:Chickens; D002681:China; D017931:DNA Primers / Q000235:genetics; D014644:Genetic Variation; D007243:Infectious bursal disease virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D008969:Molecular Sequence Data; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017386:Sequence Homology, Amino Acid; D012689:Sequence Homology, Nucleic Acid; D015678:Viral Structural Proteins / Q000235:genetics*; D014774:Virulence / Q000235:genetics
chemical_list: D017931:DNA Primers; D012367:RNA, Viral; C082513:VP2 protein, infectious bursal disease virus; D015678:Viral Structural Proteins