title: [Anti-proliferative and apoptosis-inducing activity of Scutellaria barbate containing serum on mouse's hepatoma H22 cells].
abstract: OBJECTIVE
  To investigate the effects of Scutellaria barbate extract (ESB) on suppressing proliferation and inducing apoptosis of mouse hepatoma H22 cells.
  
  
  METHODS
  H22 cells cultured in vitro were divided into 5 groups: blank control group, ESB in high, medium, low dose groups and 5-Fu group. H22 cells were cultured in media with serum containing different concentrations of ESB and blank serum. The proliferation of H22 cells was determined by microculture tetrazolium (MTT) assay. Fluorescence microscopy was utilized to observe the apoptosis of H22 cells by staining with Hoechst 33258. The cell cycle and apoptosis were analyzed by flow cytometry (FCM).
  
  
  RESULTS
  The inhibition of serum containing ESB on the proliferation of H22 cells in vitro was observerd in a dose and time dependent manner. The typical morphological changes of apoptosis were observed after incubation with ESB-containing serum in high dose for 48 hours. Among the various phases of cell cycle, the percentage of cells in S phase decreased significantly, while the percentage of cells in G1 phase increased. Drug-containing serum showed positive effect on cell apoptosis. The apoptosis rate of blank control group, ESB in low, medium, high dose groups and 5-Fu group were 0.51%, 1.07%, 3.15%, 7.83%, 11.26%, respectively.
  
  
  CONCLUSIONS
  ESB containing serum can inhibit proliferation and induce apoptosis of H22 cells in vitro.
authors.affiliation: Department of Oncology, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China. dzj0911@126.com
authors.forename: Zhi-Jun
authors.initials: ZJ
authors.lastname: Dai
authors.forename: Xiao-Xu
authors.initials: XX
authors.lastname: Liu
authors.forename: Qian
authors.initials: Q
authors.lastname: Xue
authors.forename: Zong-Zheng
authors.initials: ZZ
authors.lastname: Ji
authors.forename: Xi-Jing
authors.initials: XJ
authors.lastname: Wang
authors.forename: Hua-Feng
authors.initials: HF
authors.lastname: Kang
authors.forename: Hai-Tao
authors.initials: HT
authors.lastname: Guan
authors.forename: Xiao-Bin
authors.initials: XB
authors.lastname: Ma
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Ren
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000008:administration & dosage / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D005472:Fluorouracil / Q000494:pharmacology; D008114:Liver Neoplasms, Experimental / Q000473:pathology* / Q000648:ultrastructure; D008297:Male; D051379:Mice; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017207:Rats, Sprague-Dawley; D031363:Scutellaria / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; C524947:Scutellaria barbata extract; D005472:Fluorouracil