Application of a peptide-based PF2D platform for quantitative proteomics in disease biomarker discovery.
A peptide-based 2-D liquid phase fractionation (PF2D) system was used in a quantitative proteomic analysis of hepatocellular carcinoma. 2-D liquid maps of peptide specimens showed better resolution than those of proteins, leading to the identification of differentially expressed proteins. Peptide-based PF2D gave well-matched theoretical and experimental pI values and was proven to be a very efficient and versatile analytical tool for both large-scale profiling and quantification of phosphoproteins in disease biomarker discovery.
Lee
Hyoung-Joo
HJ
Department of Biochemistry, Yonsei Proteome Research Center and Biomedical Proteome Research Center, Yonsei University, Seoul, Korea.
Kang
Min-Jeong
MJ
Lee
Eun-Young
EY
Cho
Sang Yun
SY
Kim
Hoguen
H
Paik
Young-Ki
YK
D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism; D015415:Biomarkers / Q000032:analysis* / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000097:blood / Q000175:diagnosis* / Q000378:metabolism; D005591:Chemical Fractionation / Q000379:methods*; D002853:Chromatography, Liquid; D015180:Electrophoresis, Gel, Two-Dimensional; D006801:Humans; D008297:Male; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics / Q000379:methods*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry
D015415:Biomarkers; D010455:Peptides; D000469:Alkaline Phosphatase