title: Recent advances in DNA sensors.
abstract: The concept of DNA biosensors is sustained by the need for rapid and highly sensitive analytical tools for genetic detection. Their implementation is based on three key steps: (i) immobilization of single-stranded oligonucleotide probes onto a substrate; (ii) hybridization and (iii) reading. These steps involve complementary knowledge in various disciplinary fields such as surface physics and chemistry, molecular electrochemistry, micro-technologies, optics, electronics and biochemistry. We present here, in a non-exhaustive way, the recent advances in the two steps of immobilization and detection that rely upon increasing integration of the number of reading points or/and of the reading strategy.
authors.affiliation: Département de Chimie Moléculaire, UMR-5250, ICMG FR-2607, CNRS, Université Joseph Fourier BP 53, 38041 Grenoble Cédex 9, France. serge.cosnier@ujf-grenoble.fr
authors.forename: Serge
authors.initials: S
authors.lastname: Cosnier
authors.forename: Pascal
authors.initials: P
authors.lastname: Mailley
mesh_terms: D000818:Animals; D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D015342:DNA Probes; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D017403:In Situ Hybridization / Q000379:methods; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D015342:DNA Probes; D004247:DNA