title: Dendritic cell inhibitory and activating immunoreceptors (DCIR and DCAR) in duck: Genomic organization and expression.
abstract: C-type lectin immunoreceptor genes encoding DC inhibitory and activating receptors (DCIR and DCAR) were identified in a spleen EST library of duck (Anas platyrhynchos). These receptors are of interest for their potential as regulators of antigen presenting cells. A genomic clone was isolated and fully sequenced, containing one DCIR gene and two DCAR genes arranged in tandem order. Duck DCIR encodes an inhibitory receptor that features an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic domain. DCAR1 is a pseudogene. DCAR2 encodes an activating receptor with a positively charged residue in the transmembrane region. Full-length and alternatively spliced forms of both DCIR and DCAR2 are apparent. Duck DCIR and DCAR transcripts are preferentially expressed in immune and mucosal tissues including spleen, bursa of Fabricius, intestine and lung. Targeting these receptors on dendritic cells holds potential for breaking tolerance or for enhancing immune responses, relevant to the duck model for hepatitis B and vaccination against avian influenza.
authors.affiliation: Department of Biological Sciences, CW405 Biological Sciences Building, University of Alberta, Edmonton, Alta., Canada T6G 2E9.
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Guo
authors.forename: William G
authors.initials: WG
authors.lastname: Branton
authors.forename: Debra A
authors.initials: DA
authors.lastname: Moon
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xia
authors.forename: Megan R W
authors.initials: MR
authors.lastname: Macdonald
authors.forename: Katharine E
authors.initials: KE
authors.lastname: Magor
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003713:Dendritic Cells / Q000276:immunology* / Q000378:metabolism; D004372:Ducks / Q000235:genetics / Q000276:immunology*; D016678:Genome; D037181:Lectins, C-Type / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*; D008969:Molecular Sequence Data; D010802:Phylogeny; D011971:Receptors, Immunologic / Q000096:biosynthesis / Q000235:genetics / Q000276:immunology*
chemical_list: D037181:Lectins, C-Type; D011971:Receptors, Immunologic