[Interferon-gamma decreases the adhesiveness and invasiveness of prostate cancer cells].
OBJECTIVE
To study the effects of interferon-gamma (IFN-gamma) on the adhesiveness and invasiveness of prostate cancer cells.


METHODS
We determined the effect of IFN-gamma on the adhesiveness of prostate cancer cell lines LNCaP and PC-3 by treating the plate with fibronectin and laminin, on the invasiveness of prostate cancer cells into the artificial basal membrane consisting of Matrigel and fibronectin using the Transwell chamber, and on the expression of annexin-2 in these cells by Western blot.


RESULTS
The adhesion rates of prostate cancer cell lines LNCaP and PC-3 were 21% and 23% in the IFN-gamma treatment group, significantly lower than 46% and 40% in the untreated group (P < 0.05). The invasiveness of the cells was markedly decreased in the former as compared with the latter (P < 0.05). IFN-gamma significantly inhibited the expression of annexin-2 in prostate cancer cell lines LNCaP and PC-3 (P < 0.05).


CONCLUSIONS
IFN-gamma can decrease the adhesiveness and invasiveness of the prostate cancer cell line by down-regulating the expression of annexin-2.
Gao
Lin
L
Department of Urology, the Fourth Hospital Affiliated to Harbin Medical University, Harbin, Heilongjiang 150001, China. gaolin1121@yahoo.com.cn
Yang
De-jun
DJ
Lin
Xiang-guo
XG
Xu
Wan-hai
WH
Wang
Xiao-min
XM
D017306:Annexin A2 / Q000096:biosynthesis; D015153:Blotting, Western; D002448:Cell Adhesion / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D006801:Humans; D007371:Interferon-gamma / Q000494:pharmacology*; D008297:Male; D009361:Neoplasm Invasiveness; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology
C472822:ANXA2 protein, human; D017306:Annexin A2; D007371:Interferon-gamma