Automated analysis of fluvoxamine in rat plasma using a column-switching system and ion-pair high-performance liquid chromatography.
We have established a robust, fully automated analytical method for the analysis of fluvoxamine in rat plasma using a column-switching ion-pair high-performance chromatography system. The plasma sample was injected onto a precolumn packed with Shim-pack MAYI-ODS (50 microm), where the drug was automatically purified and enriched by on-line solid-phase extraction. After elution of the plasma proteins, the analyte was back-flushed from the precolumn and then separated isocratically on a reversed-phase C18 column (L-column ODS) with a mobile phase (acetonitrile-0.1% phosphoric acid, 36:64, v/v) containing 2 mM sodium 1-octanesulfonate. The analyte was monitored by a UV detector at a wavelength of 254 nm. The calibration line for fluvoxamine showed good linearity in the range of 5-5000 ng/mL (r > 0.999) with the limit of quantification of 5 ng/mL (RSD = 6.51%). Accuracy ranged from -2.94 to 4.82%, and the within- and between-day precision of the assay was better than 8% across the calibration range. The analytical sensitivity and accuracy of this assay is suitable for characterization of the pharmacokinetics of orally-administered fluvoxamine in rats.
Liu
Shicheng
S
Research Department, Saitama Daiichi Pharmaceutical Co., Ltd, 8-1, Minamisakae-cho, Kasukabe, Saitama 344-0057, Japan. riu@sdsk.co.jp
Shinkai
Norihiro
N
Kakubari
Ikuhiro
I
Saitoh
Hideo
H
Noguchi
Ken-ichi
K
Saitoh
Takashi
T
Yamauchi
Hitoshi
H
D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D016666:Fluvoxamine / Q000097:blood*; D051381:Rats; D015203:Reproducibility of Results
D016666:Fluvoxamine