Superparamagnetic iron oxide does not affect the viability and function of adipose-derived stem cells, and superparamagnetic iron oxide-enhanced magnetic resonance imaging identifies viable cells.
The objectives of this study were (1) to determine whether superparamagnetic iron oxide (SPIO) affects viability, transdifferentiation potential and cell-factor secretion of adipose-derived stem cells (ASCs); and (2) to determine whether SPIO-enhanced magnetic resonance (MR) imaging highlights living stem cells. Rat ASCs were incubated in SPIO-containing cell culture medium for 2 days. The SPIO-treated ASCs were then subjected to adipogenic, osteogenic and myogenic transdifferentiation. Expression of vascular endothelial growth factor, hepatocyte growth factor and insulin-like growth factor 1 by the SPIO-treated ASCs was measured using reverse transcription polymerase chain reaction. Cell viability was assessed using trypan blue stain. For in vivo experiments, SPIO-labeled ASCs were injected into 10 rat hearts. The hearts were monitored using MRI. We found that survival rate of the ASCs cultured in the SPIO-containing medium was very high (97-99%). The SPIO-treated ASCs continued to express specific markers for the three types of transdifferentiation. Expression of the cell factors by the ASCs was not affected by SPIO. Signal voids on MR images were associated with the living SPIO-labeled ASCs in the rat hearts. We conclude that SPIO does not affect viability, transdifferentiation potential or cell-factor secretion of ASCs. MRI mainly highlights living SPIO-labeled stem cells.
Wang
Lei
L
Institute for Biodiagnostics, National Research Council Canada, Winnipeg, Canada R3B 1Y6.
Deng
Jixian
J
Wang
Jian
J
Xiang
Bo
B
Yang
Tonghua
T
Gruwel
Marco
M
Kashour
Tarek
T
Tomanek
Boguslaw
B
Summer
Randy
R
Freed
Darren
D
Jassal
Davinder S
DS
Dai
Guangping
G
Glogowski
Miriam
M
Deslauriers
Roxanne
R
Arora
Rakesh C
RC
Tian
Ganghong
G
D017667:Adipocytes / Q000378:metabolism / Q000502:physiology*; D000704:Analysis of Variance; D000818:Animals; D002470:Cell Survival / Q000502:physiology*; D002478:Cells, Cultured; D003287:Contrast Media / Q000494:pharmacology*; D003911:Dextrans; D052203:Ferrosoferric Oxide; D017228:Hepatocyte Growth Factor / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D007334:Insulin-Like Growth Factor I / Q000378:metabolism; D007501:Iron / Q000494:pharmacology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D058185:Magnetite Nanoparticles; D008297:Male; D010087:Oxides / Q000494:pharmacology*; D051381:Rats; D011917:Rats, Inbred Lew; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013194:Staining and Labeling; D013234:Stem Cells / Q000378:metabolism / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism
D003287:Contrast Media; D003911:Dextrans; D058185:Magnetite Nanoparticles; D010087:Oxides; D042461:Vascular Endothelial Growth Factor A; D017228:Hepatocyte Growth Factor; D007334:Insulin-Like Growth Factor I; D007501:Iron; C065507:ferumoxides; D052203:Ferrosoferric Oxide