title: [A mass-spectrometric approach to primary screening of collagenolytic enzymes].
abstract: A comparative analysis of MALDI TOF mass spectra of low-molecular products resulting from the hydrolysis of native collagen I by collagenases of various classes (bacterial metallocollagenase from Clostridium histolyticum, serine collagenase from the Morikrasa commercial preparation, cysteine collagenase from Serratia proteomaculans, and cysteine collagenases from larvae of beetles Dermestesfrischi and D. maculates) was carried out. The spectra contain a number of ion peaks common for all collagenases; nevertheless, the mass spectra of each hydrolysate contains a unique set of peaks ("fingerprint") characteristic of each enzyme. This is especially true for the peaks of major products with relative intensities of more than 50%. At the same time, the enzymes of one class (cysteine collagenases) exhibit in their mass spectra peaks of identical major products. The results show a potential opportunity for MALDI TOF application in the primary screening of collagenases according to the fingerprints of collagen hydrolysis products.
authors.forename: Iu V
authors.initials: IuV
authors.lastname: Sungurov
authors.forename: N L
authors.initials: NL
authors.lastname: Eremeev
authors.forename: A T
authors.initials: AT
authors.lastname: Lebedev
authors.forename: O A
authors.initials: OA
authors.lastname: Maloshitskaia
authors.forename: G N
authors.initials: GN
authors.lastname: Rudenskaia
authors.forename: S A
authors.initials: SA
authors.lastname: Semenova
mesh_terms: D000818:Animals; D047009:Hathewaya histolytica / Q000201:enzymology; D001517:Coleoptera / Q000201:enzymology; D024042:Collagen Type I / Q000737:chemistry*; D017364:Collagenases / Q000737:chemistry*; D003012:Microbial Collagenase / Q000737:chemistry; D012705:Serratia / Q000201:enzymology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D024042:Collagen Type I; D017364:Collagenases; D003012:Microbial Collagenase