title: Differentiation of CD24- pancreatic ductal cell-derived cells into insulin-secreting cells.
abstract: Pancreatic progenitor cells represent both a potential source of transplantable islets for the treatment of diabetes and a valuable instrument for the investigation of the tumorigenesis of pancreatic carcinoma. It has been reported that pancreatic ductal cells of adults have the characteristics of pancreatic progenitors, but whether these cells can generate endocrine cells requires verification. Here, the differentiation of daughter cells of CD24(-) pancreatic ductal cells into insulin-secreting cells in vitro is reported. Crude pancreatic ductal cells were first obtained from adult mice by gradient centrifugation, and then the CD24(-) cells were isolated with a fluorescence-activated cell sorter. The isolated cells were cultured in serum-containing medium at clonal density to form epithelial colonies (ECs). The ECs were then stimulated with basic fibroblast growth factor (bFGF). After 72 h, insulin-secreting cells were observed in the ECs. These results indicate that the daughter cells of CD24(-) pancreatic ductal cells can differentiate into insulin-secreting cells in vitro when stimulated with exogenous bFGF. Therefore, CD24(-) pancreatic ductal cells have the potential to be pancreatic progenitor cells.
authors.affiliation: Pancreatic Center, Department of General Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan City, Hubei Province, 430022 China. chunyouwang52@126.com
authors.forename: Chun-You
authors.initials: CY
authors.lastname: Wang
authors.forename: Shan-Miao
authors.initials: SM
authors.lastname: Gou
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.forename: He-Shui
authors.initials: HS
authors.lastname: Wu
authors.forename: Jiong-Xin
authors.initials: JX
authors.lastname: Xiong
authors.forename: Feng
authors.initials: F
authors.lastname: Zhou
authors.forename: Jing
authors.initials: J
authors.lastname: Tao
mesh_terms: D053687:Adult Stem Cells / Q000166:cytology / Q000378:metabolism; D000818:Animals; D051927:CD24 Antigen / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D002469:Cell Separation; D002478:Cells, Cultured; D002999:Clone Cells; D005260:Female; D005434:Flow Cytometry; D050417:Insulin-Secreting Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D010183:Pancreatic Ducts / Q000166:cytology* / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism
chemical_list: D051927:CD24 Antigen