Evaluation of the safety of laser-induced fluorescence on the model of human diploid cell culture.
The possibility of using L-68 diploid cell culture as a model for evaluation of the safety of laser-induced fluorescence stimulated by Kr-F eximer laser (lambda=248 nm) is proven. Laser irradiation of human diploid fibroblasts in a dose of 300 mJ/cm2 and higher led to changes in cell morphology, decreased their proliferative activity and viability, and induced accumulation of lipid peroxidation products (carbonyl compounds) in the nutrient medium. Irradiation doses below 100 pulses did not modify cultural and morphological characteristics of cells.
Fatyukhina
O E
OE
Vector State Research Center of Virology and Biotechnology, Koltsovo, Novosibirsk region.
Kolokoltsova
T D
TD
Troshkova
G P
GP
D049109:Cell Proliferation / Q000528:radiation effects; D002470:Cell Survival / Q000528:radiation effects; D002478:Cells, Cultured; D004171:Diploidy; D004307:Dose-Response Relationship, Radiation; D005453:Fluorescence; D006801:Humans; D007834:Lasers; D015227:Lipid Peroxidation / Q000528:radiation effects