Genetic regulation of cervical antiinflammatory cytokine concentrations during pregnancy.
OBJECTIVE
The purpose of this study was to evaluate the contribution of maternal genotype to the concentration of interleukin (IL)-4, -10, and -13 in the cervix during the first trimester in the context of bacterial vaginosis and cigarette smoking.


METHODS
In this prospective cohort of 116 pregnant women, we examined 72 single nucleotide polymorphisms (SNPs) in 6 genes: IL-4, IL-4 receptor, IL-10, IL-10 receptor alpha, IL-10 receptor beta, and IL-13.


RESULTS
Among black women, an IL-10 receptor beta SNP (rs6517158) demonstrated significant association with IL-10 concentration (P = .04), adjusted for bacterial vaginosis. Among white women, there were 4 SNPs in IL-10 receptor alpha with a highly significant association with IL-10 concentration, adjusted for bacterial vaginosis and smoking.


CONCLUSIONS
Our data demonstrate a significant relation between cervical concentrations of IL-10 and SNPs in the IL-10 receptor alpha and beta genes. The contribution of genotype to IL-10 concentration is significant, even in the consideration of bacterial vaginosis and smoking.
Simhan
Hyagriv N
HN
Department of Obstetrics, Division of Maternal-Fetal Medicine, University of Pittsburgh School of Medicine, Magee-Womens Research Institute, Pittsburgh, PA, USA.
Ryckman
Kelli K
KK
Williams
Scott M
SM
Krohn
Marijane A
MA
D000293:Adolescent; D000328:Adult; D000704:Analysis of Variance; D002584:Cervix Uteri / Q000276:immunology / Q000378:metabolism*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism*; D053709:Interleukin-10 Receptor alpha Subunit / Q000378:metabolism; D053710:Interleukin-10 Receptor beta Subunit / Q000378:metabolism; D018793:Interleukin-13 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy; D011261:Pregnancy Trimester, First / Q000235:genetics / Q000276:immunology / Q000502:physiology*; D047928:Premature Birth / Q000235:genetics; D011446:Prospective Studies; D012907:Smoking; D016585:Vaginosis, Bacterial / Q000378:metabolism
D053709:Interleukin-10 Receptor alpha Subunit; D053710:Interleukin-10 Receptor beta Subunit; D018793:Interleukin-13; D016753:Interleukin-10; D015847:Interleukin-4