title: Hyperbaric oxygen therapy aggravates liver reperfusion injury in rats.
abstract: OBJECTIVE
  To evaluate the effects of hyperbaric oxygen (HO) therapy in the protection against liver ischemia/reperfusion injury.
  
  
  METHODS
  Thirty-two male Wistar rats were divided into four groups of eight animals each: group A - laparotomy and liver manipulation, group B - liver ischemia and reperfusion, group C - HO pretreatment for 60 min followed by liver ischemia and reperfusion, and group D - pretreatment with ambient air at 2.5 absolute atmospheres for 60 min followed by liver ischemia and reperfusion. Plasma was assayed for aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). Intra-arterial blood pressure was monitored continuously. Myeloperoxidase activity in the liver and lung was assessed 30 min after reperfusion.
  
  
  RESULTS
  Plasma AST, ALT and LDH increased after reperfusion in all animals. Plasma ALT values and myeloperoxidase activity in the liver parenchyma were higher in HO-pretreated animals than in groups A, B and D. HO had a negative hemodynamic effect during liver reperfusion.
  
  
  CONCLUSIONS
  Liver preconditioning with hyperbaric oxygen therapy aggravated liver ischemia/reperfusion injury in rats as demonstrated by plasma ALT and liver myeloperoxidase activity.
authors.affiliation: Department of Physiology, School of Science, Federal University of Minas Gerais, Brazil. cxlima@globo.com
authors.forename: Cristiano Xavier
authors.initials: CX
authors.lastname: Lima
authors.forename: Marcelo Dias
authors.initials: MD
authors.lastname: Sanches
authors.forename: João Baptista de
authors.initials: JB
authors.lastname: Rezende Neto
authors.forename: Roberto Carlos de Oliveira e
authors.initials: RC
authors.lastname: Silva
authors.forename: Mauro Martins
authors.initials: MM
authors.lastname: Teixeira
authors.forename: Danielle da Glória de
authors.initials: Dda G
authors.lastname: Souza
authors.forename: Guilherme de Castro
authors.initials: Gde C
authors.lastname: Santos
authors.forename: José Renan da Cunha
authors.initials: JR
authors.lastname: Melo
mesh_terms: D000410:Alanine Transaminase / Q000097:blood*; D000818:Animals; D001219:Aspartate Aminotransferases / Q000097:blood; D001794:Blood Pressure / Q000502:physiology; D001795:Blood Pressure Determination; D004195:Disease Models, Animal; D006931:Hyperbaric Oxygenation / Q000009:adverse effects*; D019194:Ischemic Preconditioning / Q000009:adverse effects*; D007770:L-Lactate Dehydrogenase / Q000097:blood; D008099:Liver / Q000098:blood supply*; D008107:Liver Diseases / Q000209:etiology* / Q000517:prevention & control; D008297:Male; D018384:Oxidative Stress; D051381:Rats; D017208:Rats, Wistar; D015427:Reperfusion Injury / Q000209:etiology* / Q000517:prevention & control
chemical_list: D007770:L-Lactate Dehydrogenase; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase