[Effects of psoralen plus long-wave ultraviolet-A on apoptosis of HL-60 leukemia cells].
OBJECTIVE
To explore the effects of psoralen (PSO) plus long-wave ultraviolet-A (PUVA) on apoptosis and expression of Fas ligand (FasL) in HL-60 leukemia cells.


METHODS
The HL-60 cells were taken as the study objects and their apoptosis rates, ultrastructure changes and the expression of FasL were detected in order to observe the effects of PSO and ultraviolet-A (UVA) of wave length 360 nm. The factorial design and analysis of variance were used to analyze the interaction among the factors.


RESULTS
PSO, UVA and PUVA all induced the apoptosis and the effects of PUVA were stronger than those of the other two. After HL-60 cells had been treated with PUVA, they all showed obvious ultrastructure changes due to apoptosis observed under the electron microscope. PSO, UVA and PUVA all decreased the expressions of FasL gene and protein. The effects of PUVA were stronger than those of the other two.


CONCLUSIONS
PUVA can induce the apoptosis of HL-60 cells and the effects are stronger than those of PSO or UVA alone. The expression of FasL gene in HL-60 cells is down-regulated during the apoptosis induced by PUVA.
Chen
Nan-nan
NN
Department of Traditional Chinese Medicine and Hematology, Dalian 210th Hospital of People's Liberation Army, Dalian, Liaoning Province 116021, China. chnngax@163.com
Huang
Shi-lin
SL
Xiang
Yang
Y
Zhang
Li
L
Zhang
De-jie
DJ
D017209:Apoptosis / Q000187:drug effects* / Q000528:radiation effects*; D053222:Fas Ligand Protein / Q000235:genetics / Q000378:metabolism; D005363:Ficusin / Q000494:pharmacology*; D018922:HL-60 Cells; D006801:Humans; D011701:PUVA Therapy; D017319:Photosensitizing Agents / Q000494:pharmacology*; D014466:Ultraviolet Rays
C505661:FASLG protein, human; D053222:Fas Ligand Protein; D017319:Photosensitizing Agents; D005363:Ficusin