title: Variation of acharan sulfate and monosaccharide composition and analysis of neutral N-glycans in African giant snail (Achatina fulica).
abstract: Acharan sulfate content from African giant snail (Achatina fulica) was compared in eggs and snails of different ages. Acharan sulfate was not found in egg. Acharan sulfate disaccharide -->4)-alpha-D-GlcNpAc (1-->4)-alpha-L-IdoAp2S(1-->, analyzed by SAX (strong-anion exchange)-HPLC was observed soon after hatching and increases as the snails grow. Monosaccharide compositional analysis showed that mole % of glucosamine, a major monosaccharide of acharan sulfate, increased with age while mole % of galactose decreased with age. These results suggest that galactans represent a major energy source during development, while acharan sulfate appearing immediately after hatching, is essential for the snail growth. The structures of neutral N-glycans released from eggs by peptide N-glycosidase F (PNGase F), were next elucidated using ESI-MS/MS, MALDI-MS/MS, enzyme digestion, and monosaccharide composition analysis. Three types of neutral N-glycan structures were observed, truncated (Hex(2-4)-HexNAc(2)), high mannose (Hex(5-9)-HexNAc(2)), and complex (Hex(3)-HexNAc(2-10)) types. None showed core fucosylation.
authors.affiliation: Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
authors.forename: Youmie
authors.initials: Y
authors.lastname: Park
authors.forename: Zhenqing
authors.initials: Z
authors.lastname: Zhang
authors.forename: Tatiana N
authors.initials: TN
authors.lastname: Laremore
authors.forename: Boyangzi
authors.initials: B
authors.lastname: Li
authors.forename: Joon-Soo
authors.initials: JS
authors.lastname: Sim
authors.forename: A-Rang
authors.initials: AR
authors.lastname: Im
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Ahn
authors.forename: Yeong Shik
authors.initials: YS
authors.lastname: Kim
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Linhardt
mesh_terms: D000349:Africa; D000818:Animals; D002240:Carbohydrate Sequence; D049851:Gastropoda / Q000737:chemistry* / Q000201:enzymology; D006025:Glycosaminoglycans / Q000737:chemistry*; D008358:Mannose / Q000737:chemistry; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase / Q000378:metabolism; D008969:Molecular Sequence Data; D009005:Monosaccharides / Q000737:chemistry*; D010063:Ovum / Q000737:chemistry; D011134:Polysaccharides / Q000032:analysis* / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D001619:beta-N-Acetylhexosaminidases / Q000378:metabolism
chemical_list: D006025:Glycosaminoglycans; D009005:Monosaccharides; D011134:Polysaccharides; C106771:acharan sulfate; D001619:beta-N-Acetylhexosaminidases; D017038:Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase; D008358:Mannose