title: [Changes in physicochemical properties of proteins, caused by modification with alkylhydroxybenzenes].
abstract: Kinetic characteristics of model enzymes and physicochemical properties of globular proteins modified by chemical analogues of low-molecular-weight microbial autoregulators (alkylhydroxybenzenes, AHBs) have been studied. C7 and C12 AHB homologues were used, differing in the length of the alkyl radical and the capacity for weak physicochemical interactions. Both homologues affected the degree of protein swelling, viscosity, and the degree of hydrophobicity. The effects depended on the structure of AHBs, their concentration, and pH of the solution, which likely reflects changes in the charge of the protein globule and its solvate cover. Variations of hydrophobicity indices of AHB-modified enzymes (trypsin and lysozyme) were coupled to changes in the catalytic activity. The values of K(M), measured for the enzymes within both AHB complexes, did not change, whereas V(max) increased (in the case of C7 complexes) or decreased (C12 complexes). Possible molecular mechanisms of changes in the physicochemical and catalytic parameters of enzymatically active proteins, induced by modification with structurally distinct AHBs, are described, with emphasis on targeted regulation of functional activity.
authors.forename: Iu A
authors.initials: IuA
authors.lastname: Nikolaev
authors.forename: N G
authors.initials: NG
authors.lastname: Loĭko
authors.forename: I Iu
authors.initials: IIu
authors.lastname: Stepanenko
authors.forename: E F
authors.initials: EF
authors.lastname: Shanenko
authors.forename: E I
authors.initials: EI
authors.lastname: Martirosova
authors.forename: V K
authors.initials: VK
authors.lastname: Plakunov
authors.forename: A N
authors.initials: AN
authors.lastname: Kozlova
authors.forename: I A
authors.initials: IA
authors.lastname: Borzenkov
authors.forename: O A
authors.initials: OA
authors.lastname: Korotina
authors.forename: D S
authors.initials: DS
authors.lastname: Rodin
authors.forename: Iu F
authors.initials: IuF
authors.lastname: Krupianskiĭ
authors.forename: G I
authors.initials: GI
authors.lastname: El'-Registan
mesh_terms: D004789:Enzyme Activation; D005780:Gelatin / Q000737:chemistry*; D057927:Hydrophobic and Hydrophilic Interactions; D009113:Muramidase / Q000737:chemistry*; D011487:Protein Conformation; D012118:Resorcinols / Q000737:chemistry*; D014357:Trypsin / Q000737:chemistry*; D014783:Viscosity
chemical_list: D012118:Resorcinols; D005780:Gelatin; D009113:Muramidase; D014357:Trypsin