title: Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release.
abstract: The ability of nitric oxide (NO)-releasing xerogels to reduce fibrinogen-mediated adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli is described. A negative correlation was observed between NO surface flux and bacterial adhesion for each species tested. For S. aureus and E. coli, reduced adhesion correlated directly with NO flux from 0 to 30 pmol cm(-2)s(-1). A similar dependence for S. epidermidis was evident from 18 to 30 pmol cm(-2)s(-1). At a NO flux of 30 pmol cm(-2)s(-1), surface coverage of S. aureus, S. epidermidis, and E. coli was reduced by 96, 48, and 88%, respectively, compared to non-NO-releasing controls. Polymeric NO release was thus demonstrated to be an effective approach for significantly reducing fibrinogen-mediated adhesion of both gram-positive and gram-negative bacteria in vitro, thereby illustrating the advantage of active NO release as a strategy for inhibiting bacterial adhesion in the presence of pre-adsorbed protein.
authors.affiliation: Department of Chemistry, University of North Carolina at Chapel Hill, Caudill and Kenan Laboratories, CB 3290, Chapel Hill, NC 27599-3290, USA.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Charville
authors.forename: Evan M
authors.initials: EM
authors.lastname: Hetrick
authors.forename: Carri B
authors.initials: CB
authors.lastname: Geer
authors.forename: Mark H
authors.initials: MH
authors.lastname: Schoenfisch
mesh_terms: D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage* / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000502:physiology; D005340:Fibrinogen / Q000008:administration & dosage* / Q000737:chemistry*; D008422:Materials Testing; D009569:Nitric Oxide / Q000008:administration & dosage* / Q000737:chemistry*; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000502:physiology
chemical_list: D020099:Coated Materials, Biocompatible; D009569:Nitric Oxide; D005340:Fibrinogen