title: Cycloaspeptide A and pseurotin A from the endophytic fungus Penicillium janczewskii.
abstract: Penicillium janczewskii K. M. Zalessky was isolated as an endophytic fungus from the phloem of the Chilean gymnosperm Prumnopitys andina. When grown in liquid yeast extract-malt extract-glucose broth, the fungus produced two main secondary metabolites. The compounds were for the first time isolated from this species and identified by spectroscopic methods as pseurotin A and cycloaspeptide A. This is the first report on the production of cyclic peptides by endophytic fungi from Chilean gymnosperms. Pseurotin A and cycloaspeptide A presented low cytotoxicity towards human lung fibroblasts with IC50 > or = 1000 microM. Pseurotin A showed a moderate effect against the phytopathogenic bacteria Erwinia carotovora and Pseudomonas syringae, with IC50 values of 220 and 112 microg ml(-1), respectively.
authors.affiliation: Laboratorio de Química de Productos Naturales, Instituto de Química de Recursos Naturales, Universidad de Talca, Casilla 747, Talca, Chile. schmeda@utalca.cl
authors.forename: Guillermo
authors.initials: G
authors.lastname: Schmeda-Hirschmann
authors.forename: Emilio
authors.initials: E
authors.lastname: Hormazabal
authors.forename: Jaime A
authors.initials: JA
authors.lastname: Rodriguez
authors.forename: Cristina
authors.initials: C
authors.lastname: Theoduloz
mesh_terms: D002677:Chile; D009682:Magnetic Resonance Spectroscopy; D008958:Models, Molecular; D010407:Penicillium / Q000737:chemistry* / Q000302:isolation & purification; D010456:Peptides, Cyclic / Q000737:chemistry / Q000302:isolation & purification*; D011760:Pyrrolidinones / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D010456:Peptides, Cyclic; D011760:Pyrrolidinones; C500117:cycloaspeptide A; C012013:pseurotin