title: Cell-specific ATP7A transport sustains copper-dependent tyrosinase activity in melanosomes.
abstract: Copper is a cofactor for many cellular enzymes and transporters. It can be loaded onto secreted and endomembrane cuproproteins by translocation from the cytosol into membrane-bound organelles by ATP7A or ATP7B transporters, the genes for which are mutated in the copper imbalance syndromes Menkes disease and Wilson disease, respectively. Endomembrane cuproproteins are thought to incorporate copper stably on transit through the trans-Golgi network, in which ATP7A accumulates by dynamic cycling through early endocytic compartments. Here we show that the pigment-cell-specific cuproenzyme tyrosinase acquires copper only transiently and inefficiently within the trans-Golgi network of mouse melanocytes. To catalyse melanin synthesis, tyrosinase is subsequently reloaded with copper within specialized organelles called melanosomes. Copper is supplied to melanosomes by ATP7A, a cohort of which localizes to melanosomes in a biogenesis of lysosome-related organelles complex-1 (BLOC-1)-dependent manner. These results indicate that cell-type-specific localization of a metal transporter is required to sustain metallation of an endomembrane cuproenzyme, providing a mechanism for exquisite spatial control of metalloenzyme activity. Moreover, because BLOC-1 subunits are mutated in subtypes of the genetic disease Hermansky-Pudlak syndrome, these results also show that defects in copper transporter localization contribute to hypopigmentation, and hence perhaps other systemic defects, in Hermansky-Pudlak syndrome.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
authors.forename: Subba Rao Gangi
authors.initials: SR
authors.lastname: Setty
authors.forename: Danièle
authors.initials: D
authors.lastname: Tenza
authors.forename: Elena V
authors.initials: EV
authors.lastname: Sviderskaya
authors.forename: Dorothy C
authors.initials: DC
authors.lastname: Bennett
authors.forename: Graça
authors.initials: G
authors.lastname: Raposo
authors.forename: Michael S
authors.initials: MS
authors.lastname: Marks
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D027682:Cation Transport Proteins / Q000378:metabolism*; D002460:Cell Line; D003300:Copper / Q000378:metabolism* / Q000494:pharmacology; D000073840:Copper-Transporting ATPases; D011992:Endosomes / Q000378:metabolism; D006801:Humans; D008544:Melanocytes / Q000166:cytology / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D020460:Melanosomes / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism; D051379:Mice; D014442:Monophenol Monooxygenase / Q000378:metabolism*; D009928:Organ Specificity; D050765:Qa-SNARE Proteins / Q000378:metabolism; D014443:Tyrosine / Q000378:metabolism*; D021601:trans-Golgi Network / Q000378:metabolism
chemical_list: C486201:Atp7a protein, mouse; D002352:Carrier Proteins; D027682:Cation Transport Proteins; D050765:Qa-SNARE Proteins; D014443:Tyrosine; D003300:Copper; D014442:Monophenol Monooxygenase; D000251:Adenosine Triphosphatases; D000073840:Copper-Transporting ATPases