Spatial and temporal aspects of cAMP signalling in cardiac myocytes.
1. beta(1)-Adrenoceptor and M(2) muscarinic receptor regulation of cAMP production plays a pivotal role in autonomic regulation of cardiac myocyte function. However, not all responses are easily explained by a uniform increase or decrease in cAMP activity throughout the entire cell. 2. Adenovirus expression of fluorescence resonance energy transfer (FRET)-based biosensors can be used to monitor cAMP activity in protein kinase A (PKA) signalling domains, as well as the bulk cytoplasmic domain of intact adult cardiac myocytes. 3. Data obtained using FRET-based biosensors expressed in different cellular microdomains have been used to develop a computational model of compartmentalized cAMP signalling. 4. A systems biology approach that uses quantitative computational modelling together with experimental data obtained using FRET-based biosensors has been used to provide evidence for the idea that compartmentation of cAMP signalling is necessary to explain the stimulatory responses to beta(1)-adrenoceptor activation as well as the complex temporal responses to M(2) muscarinic receptor activation.
Iancu
Radu V
RV
Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, Ohio, USA.
Ramamurthy
Gopalakrishnan
G
Harvey
Robert D
RD
D000818:Animals; D019295:Computational Biology / Q000379:methods / Q000639:trends; D000242:Cyclic AMP / Q000502:physiology*; D006801:Humans; D032383:Myocytes, Cardiac / Q000166:cytology / Q000502:physiology*; D015398:Signal Transduction / Q000502:physiology*; D013997:Time Factors
D000242:Cyclic AMP