Genomic analysis of the role of RNase R in the turnover of Pseudomonas putida mRNAs.
RNase R is a 3'-5' highly processive exoribonuclease that can digest RNAs with extensive secondary structure. We analyzed the global effect of eliminating RNase R on the Pseudomonas putida transcriptome and the expression of the rnr gene under diverse conditions. The absence of RNase R led to increased levels of many mRNAs, indicating that it plays an important role in mRNA turnover.
Fonseca
Pilar
P
Departamento de Biotecnología Microbiana, Centro Nacional de Biotecnología, CSIC, Campus de la Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.
Moreno
Renata
R
Rojo
Fernando
F
D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D005095:Exoribonucleases / Q000235:genetics / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial; D020868:Gene Silencing; D023281:Genomics; D020411:Oligonucleotide Array Sequence Analysis; D016958:Pseudomonas putida / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020871:RNA Stability; D012329:RNA, Bacterial / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*
D001426:Bacterial Proteins; D012329:RNA, Bacterial; D012333:RNA, Messenger; D005095:Exoribonucleases; C080868:ribonuclease R