Synthetic "chaperones": nanoparticle-mediated refolding of thermally denatured proteins.
Thermally denatured chymotrypsin, lysozyme and papain are substantially refolded towards their native conformation by gold nanoparticle bearing dicarboxylate sidechains.
De
Mrinmoy
M
Department of Chemistry, University of Massachusetts at Amherst, Amherst, MA 01003, USA.
Rotello
Vincent M
VM
D002942:Circular Dichroism; D006046:Gold / Q000737:chemistry* / Q000494:pharmacology*; D006358:Hot Temperature; D006867:Hydrolases / Q000737:chemistry / Q000378:metabolism; D057927:Hydrophobic and Hydrophilic Interactions; D053768:Metal Nanoparticles / Q000737:chemistry*; D008958:Models, Molecular; D011487:Protein Conformation; D011489:Protein Denaturation; D017510:Protein Folding; D020673:Protein Renaturation / Q000187:drug effects*
D006046:Gold; D006867:Hydrolases