Digoxin facilitates neuromuscular transmission in mouse diaphragm.
Low concentration of digoxin (3 nM) facilitated spontaneous and evoked release of neurotransmitter acetylcholine thereby increasing the frequency of miniature end-plate potentials, amplitude of single end-plate potentials, their quantum content and the plateau level in the bursts during stimulation of the phrenic nerve at rates of 4, 7, and 50 Hz. These effects were prevented by blockade of ryanodine receptors with ryanodine (10-20 microM).
Balezina
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OP
Department of Human and Animal Physiology, Biological Faculty, M. V. Lomonosov Moscow State University. balezina@mail.ru
Lapteva
V I
VI
D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology; D003964:Diaphragm / Q000187:drug effects* / Q000294:innervation / Q000502:physiology; D004077:Digoxin / Q000494:pharmacology*; D005071:Evoked Potentials / Q000187:drug effects; D066298:In Vitro Techniques; D051379:Mice; D009046:Motor Neurons / Q000187:drug effects / Q000502:physiology; D009469:Neuromuscular Junction / Q000187:drug effects* / Q000502:physiology; D010791:Phrenic Nerve / Q000187:drug effects / Q000502:physiology; D012433:Ryanodine / Q000494:pharmacology; D019837:Ryanodine Receptor Calcium Release Channel / Q000502:physiology; D009435:Synaptic Transmission / Q000187:drug effects*
D002316:Cardiotonic Agents; D019837:Ryanodine Receptor Calcium Release Channel; D012433:Ryanodine; D004077:Digoxin