[Different change of olive tail moment and cytokinesis-block micronucleus frequency of the cells of 4 kinds treated with polycyclic aromatic hydrocarbon].
OBJECTIVE
To investigate the difference change of olive tail moment (TM) and cytokinesis-block micronucleus (CBMN) frequency of the cells of four kinds treated with polycyclic aromatic hydrocarbon.


METHODS
Four different cell lines, CHO-K1, NIH3T3, CHL, and HELF were treated with B(a)P in vitro, and the genetic damage were detected by Comet and CBMN assay, respectively. The positive controls were Hydro peroxides (H2O2 ) and Cyclohosphamide (CP) for Comet and CBMN assay, respectively.


RESULTS
The changes of olive TM levels of four cell lines treated with B(a)P were occurred at the lower doses of B(a)P (CHO - K1:0.5 micromol/L, NIH3T3 : 0.75 micromol/L, CHL : 0.5 micromol/L, HELF : 0.3125 micromol/L) than that of CBMN frequencies (CHO-K1 : 2.0 micromol/L, NIH3T3 : 1.51 micromol/L, CHL : 2.0 micromol/L, HELF : 1.25 micromol/L). The dose-response curve of B(a)P was linear for Olive TM, but sigmoid for CBMN frequency.


CONCLUSIONS
The data showed that olive TM was more sensitive than CBMN frequency for the genotoxic detects of B(a)P for the four cell lines.
Cheng
Juan
J
National Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Leng
Shuguang
S
Niu
Yong
Y
Duan
Huawei
H
D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity; D016466:CHO Cells; D002460:Cell Line; D020552:Comet Assay; D006224:Cricetinae; D003412:Cricetulus; D048749:Cytokinesis / Q000187:drug effects*; D004249:DNA Damage / Q000187:drug effects*; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D008168:Lung / Q000166:cytology; D051379:Mice; D015162:Micronucleus Tests; D041681:NIH 3T3 Cells; D011084:Polycyclic Aromatic Hydrocarbons / Q000633:toxicity*
D011084:Polycyclic Aromatic Hydrocarbons; D001564:Benzo(a)pyrene