title: Detection of mercury-, arsenic-, and selenium-containing proteins in fish liver from a mercury polluted area of Guizhou Province, China.
abstract: Mercury (Hg)-, selenium (Se)-, and arsenic (As)- containing proteins in liver tissues of bighead carp and grass carp sampled from a mercury-polluted area of Wanshan, Guizhou Province, China, were separated by thin-layer isoelectric focusing. The relative content of Hg, Se, and As in protein bands was measured with synchrotron radiation x-ray fluorescence. The results indicated that there were at least 3 Hg-containing bands with pI 3.7, 4.8, and 6.2 in liver of bighead carp and 1 Hg-containing band with pI 6.2 in grass carp. Se and As were found in the Hg-containing bands 3.7 in bighead carp and 6.2 in grass carp. The bands may be corresponding to the antagonistic effect of Se against the toxicity of Hg and As. In addition, Hg and As often coexist in the same band, suggesting that the two elements may be involved in the same metabolic processes.
authors.affiliation: Key Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Li
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.forename: Jin
authors.initials: J
authors.lastname: Sun
authors.forename: Bai
authors.initials: B
authors.lastname: Li
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.forename: Chunying
authors.initials: C
authors.lastname: Chen
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Chai
authors.forename: Atsuo
authors.initials: A
authors.lastname: Iida
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Gao
mesh_terms: D000818:Animals; D001151:Arsenic / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D002347:Carps / Q000378:metabolism*; D002681:China; D004577:Electron Probe Microanalysis; D015180:Electrophoresis, Gel, Two-Dimensional; D004784:Environmental Monitoring; D004785:Environmental Pollutants / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008099:Liver / Q000737:chemistry / Q000378:metabolism*; D008628:Mercury / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*; D008667:Metalloproteins / Q000032:analysis / Q000378:metabolism*; D012643:Selenium / Q000032:analysis / Q000737:chemistry / Q000378:metabolism*
chemical_list: D004785:Environmental Pollutants; D008667:Metalloproteins; D008628:Mercury; D012643:Selenium; D001151:Arsenic