Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release.
The ability of nitric oxide (NO)-releasing xerogels to reduce fibrinogen-mediated adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli is described. A negative correlation was observed between NO surface flux and bacterial adhesion for each species tested. For S. aureus and E. coli, reduced adhesion correlated directly with NO flux from 0 to 30 pmol cm(-2)s(-1). A similar dependence for S. epidermidis was evident from 18 to 30 pmol cm(-2)s(-1). At a NO flux of 30 pmol cm(-2)s(-1), surface coverage of S. aureus, S. epidermidis, and E. coli was reduced by 96, 48, and 88%, respectively, compared to non-NO-releasing controls. Polymeric NO release was thus demonstrated to be an effective approach for significantly reducing fibrinogen-mediated adhesion of both gram-positive and gram-negative bacteria in vitro, thereby illustrating the advantage of active NO release as a strategy for inhibiting bacterial adhesion in the presence of pre-adsorbed protein.
Charville
Gregory W
GW
Department of Chemistry, University of North Carolina at Chapel Hill, Caudill and Kenan Laboratories, CB 3290, Chapel Hill, NC 27599-3290, USA.
Hetrick
Evan M
EM
Geer
Carri B
CB
Schoenfisch
Mark H
MH
D000327:Adsorption; D001422:Bacterial Adhesion / Q000187:drug effects*; D020099:Coated Materials, Biocompatible / Q000008:administration & dosage* / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000502:physiology; D005340:Fibrinogen / Q000008:administration & dosage* / Q000737:chemistry*; D008422:Materials Testing; D009569:Nitric Oxide / Q000008:administration & dosage* / Q000737:chemistry*; D011485:Protein Binding; D013211:Staphylococcus aureus / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000502:physiology
D020099:Coated Materials, Biocompatible; D009569:Nitric Oxide; D005340:Fibrinogen