title: Biodegradable amphiphilic copolymers based on poly(epsilon-caprolactone)-graft chondroitin sulfate as drug carriers.
abstract: The goal of this study was to develop a new type of core-shell micelles based on biocompatible and biodegradable amphiphilic copolymers, named PCL-CS, using chondroitin sulfate (CS) as a hydrophilic segment and poly(epsilon-caprolactone) (PCL) as a hydrophobic segment. The copolymers, prepared from the various compositions between CS and PCL, were characterized by Fourier transform infrared spectrometer, proton nuclear magnetic resonance spectrometer, and differential scanning calorimeter. The PCL-CS copolymers could be assembled into micelles using a simple emulsion. With the fluorescent probe technique, the critical micelle concentrations were obtained in the range of 1.26 x 10(-3)-8.86 x 10(-3) mg/mL. The spherical images of micelles were visualized in the presence of polyvinyl alcohol (PVA) with the use of the transmission electron microscope. The particle sizes of micelles were all smaller than 300 nm, neither aggregate nor change in hydrodynamic sizes after 15 days staying in solutions containing salts or PVA by dynamic light scattering. The intracellular uptake of KB cells incubated with PCL-CS micelles was evidenced by confocal laser scanning microscope upon loading fluorescein isothiocyanate labeled bovine serum albumin as a probe.
authors.affiliation: Faculty of Medicinal and Applied Chemistry, School of Life Science, Kaohsiung Medical University, Kaohsiung City 80708, Taiwan.
authors.forename: Ai-Ling
authors.initials: AL
authors.lastname: Chen
authors.forename: Hsiao-Chen
authors.initials: HC
authors.lastname: Ni
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Wang
authors.forename: Jenn-Shing
authors.initials: JS
authors.lastname: Chen
mesh_terms: D001672:Biocompatible Materials / Q000138:chemical synthesis* / Q000737:chemistry*; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D004337:Drug Carriers / Q000138:chemical synthesis* / Q000737:chemistry*; D004655:Emulsions / Q000138:chemical synthesis / Q000737:chemistry; D016650:Fluorescein-5-isothiocyanate / Q000031:analogs & derivatives / Q000737:chemistry; D005456:Fluorescent Dyes / Q000737:chemistry; D006801:Humans; D008422:Materials Testing; D008823:Micelles; D015394:Molecular Structure; D010316:Particle Size; D011091:Polyesters / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013329:Structure-Activity Relationship; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D004337:Drug Carriers; D004655:Emulsions; D005456:Fluorescent Dyes; D008823:Micelles; D011091:Polyesters; C039217:fluorescein isothiocyanate bovine serum albumin; C016240:polycaprolactone; D012710:Serum Albumin, Bovine; D002809:Chondroitin Sulfates; D016650:Fluorescein-5-isothiocyanate