title: [Obtaining of the mutant Yarrowia lipolytica strains producing citric acid from glucose].
abstract: The possibility of obtaining mutant yeasts Yarrowia lipolytica VKM Y-2373 with increased ability to synthesize citric acid from glucose by using UV irradiation and N-methyl-NT-nitro-N-nitrosoguanidine was studied. Of 1500 colonies of the Y. lipolytica treated with either UV or N-methyl-NT-nitro-N-nitrosoguanidine, three mutants were selected that displayed higher (by 23%) biosynthetic ability as compared with the initial strain. Additionally, three mutants were selected from 1000 colonies of the Y. lipolytica exposed to a combined action of UV and N-methyl-NT-nitro-N-nitrosoguanidine; their biosynthetic activity exceeded that of the initial strain by 43.9%. The selective media with citrate and acetate were developed for a rapid selection of mutants as well as the express methods for the detection of active citric acid producers on the solid media with chalk and bromocresol containing a limiting concentration of amine nitrogen and an excess of glucose.
authors.forename: T V
authors.initials: TV
authors.lastname: Finogenova
authors.forename: I F
authors.initials: IF
authors.lastname: Puntus
authors.forename: S V
authors.initials: SV
authors.lastname: Kamzolova
authors.forename: Iu N
authors.initials: IuN
authors.lastname: Lunina
authors.forename: S E
authors.initials: SE
authors.lastname: Monastyrskaia
authors.forename: I G
authors.initials: IG
authors.lastname: Morgunov
authors.forename: A M
authors.initials: AM
authors.lastname: Boronin
mesh_terms: D000085:Acetates / Q000378:metabolism; D002951:Citrates / Q000378:metabolism; D019343:Citric Acid / Q000378:metabolism*; D005947:Glucose / Q000378:metabolism*; D008769:Methylnitronitrosoguanidine / Q000494:pharmacology; D009153:Mutagens / Q000494:pharmacology; D009154:Mutation; D014466:Ultraviolet Rays; D025062:Yarrowia / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects
chemical_list: D000085:Acetates; D002951:Citrates; D009153:Mutagens; D008769:Methylnitronitrosoguanidine; D019343:Citric Acid; D005947:Glucose