The anti-allergic activity of the acetate fraction of Schinus terebinthifolius leaves in IgE induced mice paw edema and pleurisy.
Schinus is a genus of the Anacardiaceae family and contains Schinus terebinthifolius, the Brazilian pepper tree that is widely used in folk medicine. We investigate the anti-allergic activity of the ethyl acetate fraction of S. terebinthifolius Raddi (ST fraction). HPLC analysis reveled that gallic acid, methyl gallate and 1,2,3,4,6-pentagalloylglucose are the major aromatic components of the fraction. Oral pre-treatment with the ST fraction (100 mg/kg) significantly inhibited paw edema induced by compound 48/80 (100 ng/paw) and to a lesser extent, the allergic paw edema (OVA, 3 microg/paw). The ST fraction (100 and 200 mg/kg) also inhibited the edema induced by histamine (100 microg/paw), preventing mast cell degranulation and, consequently, histamine release in Wistar rat peritoneal mast cells induced by C 48/80 (5 microg/mL). This histamine inhibition was also observed after mast cell pre-treatment with both methyl gallate and 1,2,3,4,6-pentagalloylglucose (100 microg/mL), the isolated compounds from the ethyl acetate fraction. Pre-treatment with the ST fraction (100 mg/kg) significantly inhibited total leukocyte and eosinophil accumulation in pleural cavities 24 h after the intrathoracic injection of OVA (12.5 microg/cavity). This effect was related to the inhibition of CCL11/eotaxin and CCL5/RANTES in pleural lavage fluid. Pre-treatment with this fraction (100 mg/kg) failed to reduce the cell influx that was observed after LPS-injection into pleural cavity (250 ng/cavity). These findings demonstrate the anti-allergic effect of the ST fraction, which includes the inhibition of edema formation and histamine release caused by mast cell degranulation and eosinophil influx into the pleural cavity probably reflected by the decreased levels of chemokines in recovered pleural lavage fluid.
Cavalher-Machado
Simone Campos
SC
Laboratório de Farmacologia Aplicada, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brazil.
Rosas
Elaine Cruz
EC
Brito
Fabiola de Almeida
Fde A
Heringe
Alan Patrick
AP
de Oliveira
Rodrigo Rodrigues
RR
Kaplan
Maria Auxiliadora Coelho
MA
Figueiredo
Maria Raquel
MR
Henriques
Maria das Graças Müller de Oliveira
Md
D027926:Anacardiaceae / Q000737:chemistry*; D000818:Animals; D018926:Anti-Allergic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D018925:Chemokines / Q000187:drug effects / Q000276:immunology; D004487:Edema / Q000188:drug therapy* / Q000276:immunology; D006632:Histamine / Q000008:administration & dosage / Q000494:pharmacology; D006636:Histamine Release / Q000187:drug effects / Q000276:immunology; D006967:Hypersensitivity / Q000188:drug therapy* / Q000276:immunology; D007073:Immunoglobulin E / Q000187:drug effects / Q000276:immunology; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D008407:Mast Cells / Q000276:immunology / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000008:administration & dosage / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000627:therapeutic use*; D018515:Plant Leaves / Q000737:chemistry*; D010998:Pleurisy / Q000188:drug therapy* / Q000276:immunology; D011398:Promethazine / Q000008:administration & dosage / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
D018926:Anti-Allergic Agents; D018925:Chemokines; D008070:Lipopolysaccharides; D010936:Plant Extracts; D007073:Immunoglobulin E; D006632:Histamine; D010047:Ovalbumin; D011398:Promethazine