title: Dioxin interferes in chromosomal positioning through the aryl hydrocarbon receptor.
abstract: Each chromosome occupies its own-specific space called a 'territory' within the interphase nucleus, and the arrangement of chromosome territories (CTs) is important in epigenetic mechanisms. The molecular mechanism to determine the positioning of CTs, however, remains unknown. On the other hand, dioxin is known to be the typical environmental pollutant that affects a wide variety of biological events in many species. Here, we show that dioxin enlarges the minimum distance between chromosome 12 and chromosome 16 territories in human preadipocyte cells, and the alteration of chromosome positioning is canceled by an aryl hydrocarbon receptor (AhR) antagonist alpha-naphthoflavone. Thus, AhR may be a key molecule to regulate chromosome positioning. Our results suggest a novel effect of dioxin toxicity, and demonstrate a clue to reveal the novel molecular mechanism for the arrangement of CTs.
authors.affiliation: Department of Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan.
authors.forename: Kosuke
authors.initials: K
authors.lastname: Oikawa
authors.forename: Keiichi
authors.initials: K
authors.lastname: Yoshida
authors.forename: Masakatsu
authors.initials: M
authors.lastname: Takanashi
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tanabe
authors.forename: Tomoharu
authors.initials: T
authors.lastname: Kiyuna
authors.forename: Maki
authors.initials: M
authors.lastname: Ogura
authors.forename: Akira
authors.initials: A
authors.lastname: Saito
authors.forename: Akihiro
authors.initials: A
authors.lastname: Umezawa
authors.forename: Masahiko
authors.initials: M
authors.lastname: Kuroda
mesh_terms: D001571:Benzoflavones / Q000494:pharmacology; D002460:Cell Line; D002467:Cell Nucleus / Q000187:drug effects; D045584:Chromosome Positioning / Q000187:drug effects*; D002881:Chromosomes, Human, Pair 12 / Q000187:drug effects*; D002885:Chromosomes, Human, Pair 16 / Q000187:drug effects*; D006801:Humans; D007399:Interphase / Q000187:drug effects; D000072317:Polychlorinated Dibenzodioxins / Q000633:toxicity*; D018336:Receptors, Aryl Hydrocarbon / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D001571:Benzoflavones; D000072317:Polychlorinated Dibenzodioxins; D018336:Receptors, Aryl Hydrocarbon; C011512:alpha-naphthoflavone