[Retroposons of Alu family from cis-regulatory modules of DNAse II and CAML genes effect gene expression in A549 and HEK293 cells].
Different fragments of promoters of deoxyribonuclease II (DNAse II) and calcium-modulating cyclophilin ligand (CAML) associated with Alu family repeats have been inserted into a luciferase reporter vector. These constructions were introduced into A549 and HEK293 cell lines and after transient transfection we lysed cells and analysed luciferase activities in these lysates. It has been shown that Alu repeats localized in constructions influence expression of luciferase. Therefore, Alu copies which are associated with cis-regulatory modules of protein-coding genes have biological activity.
Usmanova
N M
NM
Kazakov
V I
VI
Tomilin
N V
NV
D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D020087:Alu Elements / Q000235:genetics*; D002460:Cell Line; D004706:Endodeoxyribonucleases / Q000235:genetics; D005786:Gene Expression Regulation; D006801:Humans; D018626:Retroelements / Q000235:genetics*; D014158:Transcription, Genetic
D048868:Adaptor Proteins, Signal Transducing; C089404:CAMLG protein, human; D018626:Retroelements; D004706:Endodeoxyribonucleases; C019329:deoxyribonuclease II