Effects of gamma-hexachlorocyclohexane on apoptosis induced by serum deprivation in PC12 cells.
The effects of gamma-hexachlorocyclohexane, one of the endocrine disrupters, widely used for agricultural and medicinal purpose, on apoptosis in PC12 cells were investigated using western blotting analysis and reverse transcriptase-DNA polymerase chain reaction (RT-PCR) method. Apoptosis is a fundamental process necessary for development of individuals and organs. Although gamma -HCH at high concentration did not have an effect on cell viability and apoptosis, DNA fragmentation was slightly enhanced, and apoptotic factors; Bax, Bad, cytochrome c and caspase-3 showed tendency to increase by the addition of a low dose of gamma-HCH to the cell medium. However these changes were not statistically significant. It was concluded that gamma -HCH did not affect on apoptosis in the PC12 cell line system, although gamma -HCH has been reported to induce apoptosis in other cell lines.
Aoki
Kentaro
K
Graduate School of Environmental Science, Hokkaido University, Sapporo, Japan.
Egawa
Mari
M
Saito
Takeshi
T
Hosokawa
Toshiyuki
T
Kurasaki
Masaaki
M
D000818:Animals; D017209:Apoptosis / Q000187:drug effects* / Q000502:physiology; D015153:Blotting, Western; D020169:Caspases / Q000097:blood; D045304:Cytochromes c / Q000097:blood; D001556:Hexachlorocyclohexane / Q000633:toxicity*; D007306:Insecticides / Q000633:toxicity*; D016716:PC12 Cells / Q000187:drug effects* / Q000378:metabolism; D051381:Rats; D020133:Reverse Transcriptase Polymerase Chain Reaction; D051028:bcl-2-Associated X Protein / Q000097:blood
D007306:Insecticides; D051028:bcl-2-Associated X Protein; D001556:Hexachlorocyclohexane; D045304:Cytochromes c; D020169:Caspases