title: Concentration control for protein crystallization via a continuously-fed crystallization chamber.
abstract: A continuously-fed crystallization chamber that allows for kinetic path control through the crystallization phase diagram (from labile/nucleation to metastable/growth) was fabricated and used to crystallize lysozyme. A lumped kinetic model was developed, and parameters for heterogeneous nucleation kinetics were determined. Heterogeneous nucleation was found to have faster nucleation kinetics and slower growth kinetics than homogeneous nucleation, as expected. The major contributions of the new device are (1) to allow better control of the chemical environment for studies of crystal nucleation and growth, and (2) to allow lumped-model analysis of those studies to extract kinetic parameters.
authors.affiliation: Department of Chemical Engineering, University of Minnesota, 421 Washington Avenue SE, Minneapolis, MN 55455, USA. sugiy002@umn.edu
authors.forename: Masano
authors.initials: M
authors.lastname: Sugiyama
authors.forename: Shramik
authors.initials: S
authors.lastname: Sengupta
authors.forename: Paul
authors.initials: P
authors.lastname: Todd
authors.forename: Victor H
authors.initials: VH
authors.lastname: Barocas
mesh_terms: D003460:Crystallization / Q000295:instrumentation* / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000737:chemistry*
chemical_list: D011506:Proteins