The suitability of cytochrome-P4501A1 as a biomarker for PCB contamination in European eel (Anguilla anguilla).
European eel (Anguilla anguilla) elvers were intraperitoneally injected with different doses of 3,3',4,4'-tetrachlorobiyphenyl (PCB77) to examine and characterize the inductive effect of coplanar PCBs on CYP1A1 gene expression in liver and gills by using a semi-quantitative RT-PCR analysis. The influence of PCB77 injection on transcription activity of the housekeeping gene glyceraldehyde-3-phosphate-dehydrogenase (GAPDH) was tested to determine its suitability as a reference gene for further quantitative gene expression analyses. Our results clearly indicate a significant dose-dependent increase in CYP1A1 gene expression in the gills of European eel, while in liver tissues a significant elevation in CYP1A1 gene expression was only detectable at highest contamination rates, indicating the potential of CYP1A1 differential gene expression analysis in gills as a biomarker for PCB contamination in eels. PCB77 contamination did not affect GAPDH transcription in gills but, at highest doses, resulted in a significant elevation in liver, speaking against GAPDH as a reference housekeeping gene after PCB exposure.
Marohn
L
L
Leibniz Institute of Marine Sciences, Duesternbrooker Weg 20, 24105 Kiel, Germany.
Rehbein
H
H
Kündiger
R
R
Hanel
R
R
D000811:Anguilla; D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics* / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004784:Environmental Monitoring / Q000379:methods; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005880:Gills / Q000187:drug effects / Q000201:enzymology*; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000201:enzymology*; D011078:Polychlorinated Biphenyls / Q000633:toxicity*; D012333:RNA, Messenger / Q000032:analysis / Q000096:biosynthesis; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014873:Water Pollutants / Q000633:toxicity
D015415:Biomarkers; D012333:RNA, Messenger; D014873:Water Pollutants; D011078:Polychlorinated Biphenyls; D019363:Cytochrome P-450 CYP1A1; D005987:Glyceraldehyde-3-Phosphate Dehydrogenases; C028451:3,4,3',4'-tetrachlorobiphenyl