title: SV40 vectors carrying minimal sequence of viral origin with exchangeable capsids.
abstract: Polyomaviral vectors are generated by transfecting 293T cells with three sets of DNAs: DNA for the expression of simian virus 40 (SV40) T antigen; DNA for the expression of SV40 capsid proteins, and vector DNA harboring a reporter gene expression cassette carrying a SV40 origin. The vector DNA harbors a minimal sequence originating from SV40, and thus can carry a longer transgene. Moreover, the viable recombinants are not detectable in the vector preparation, and the vectors can transduce the DNA with efficiency similar to that of virions. Vector particles bearing capsid proteins of BK virus, JC virus, and B-lymphotropic papovavirus instead of SV40 were prepared, and they exhibited differential efficiency of gene transduction to the target cells. This method can be used to develop a surrogate system to study the functions of capsid proteins of polyomaviruses and to generate a set of polyomaviral vectors targeted at specific cell types.
authors.affiliation: National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8522, Japan. nakanish@nils.go.jp
authors.forename: Akira
authors.initials: A
authors.lastname: Nakanishi
authors.forename: Benoit
authors.initials: B
authors.lastname: Chapellier
authors.forename: Naoya
authors.initials: N
authors.lastname: Maekawa
authors.forename: Masaki
authors.initials: M
authors.lastname: Hiramoto
authors.forename: Takeshi
authors.initials: T
authors.lastname: Kuge
authors.forename: Ryo-u
authors.initials: RU
authors.lastname: Takahashi
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Handa
authors.forename: Takeshi
authors.initials: T
authors.lastname: Imai
mesh_terms: D000952:Antigens, Polyomavirus Transforming / Q000235:genetics; D001739:BK Virus / Q000235:genetics; D036022:Capsid Proteins / Q000235:genetics*; D017930:Genes, Reporter; D005822:Genetic Vectors; D007577:JC Virus / Q000235:genetics; D018741:Replication Origin; D013539:Simian virus 40 / Q000235:genetics*; D014161:Transduction, Genetic; D014773:Virology / Q000379:methods*
chemical_list: D000952:Antigens, Polyomavirus Transforming; D036022:Capsid Proteins