title: An optimized method for in vitro exposure of human derived lung cells to volatile chemicals.
abstract: Volatile organic compounds (VOCs) such as benzene and toluene, and low molecular weight carbonyls like formaldehyde belong to the main air pollutants found in indoor environments. They are suspected to induce acute and chronic adverse health effects like asthma, allergic and cardiovascular diseases, and strongly affect well-being. Our aim was to further develop and optimize an in vitro method to study the exposure of epithelial tumour lung cells (A549) by using a commercial exposure chamber (CULTEX) to assess the biological effects of VOCs and carbonyl compounds at low concentration levels. Exposing the cells to toluene, benzene and formaldehyde at mixing ratios varying from 0.1 to 0.6ppmv in air resulted in reproducible direct effects with the induction of an inflammatory response and a modification of the glutathione redox status.
authors.affiliation: Institute for Health and Consumer Protection, Ispra (VA), Italy. fabrizio.pariselli@jrc.it
authors.forename: F
authors.initials: F
authors.lastname: Pariselli
authors.forename: M G
authors.initials: MG
authors.lastname: Sacco
authors.forename: D
authors.initials: D
authors.lastname: Rembges
mesh_terms: D016902:Air Pollution, Indoor / Q000009:adverse effects; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000187:drug effects / Q000473:pathology; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000139:chemically induced; D016209:Interleukin-8 / Q000378:metabolism; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D020545:Respiratory Mucosa / Q000187:drug effects* / Q000378:metabolism; D055549:Volatile Organic Compounds / Q000633:toxicity*
chemical_list: D016209:Interleukin-8; D055549:Volatile Organic Compounds; D007770:L-Lactate Dehydrogenase; D005978:Glutathione