Diagnostic and prognostic implications of microRNAs in human hepatocellular carcinoma.
MicroRNAs (miRNAs) are important gene regulators, which are often deregulated in cancers. In this study, the authors analyzed the microRNAs profiles of 78 matched cancer/noncanerous liver tissues from HCC patients and 10 normal liver tissues and found that 69 miRNAs were differentially expressed between hepatocellular carcinoma (HCC) and corresponding noncancerous liver tissues (N). Then the expressions of 8 differentially expressed miRNAs were validated by real time RT PCR. The set of differentially expressed miRNAs could distinctly classify HCC, N and normal liver tissues (NL). Moreover, some of these differentially expressed miRNAs were related to the clinical factors of HCC patients. Most importantly, Kaplan-Meier estimates and the log-rank test showed that high expression of hsa-miR-125b was correlated with good survival of HCC patients (hazard ratio, 1.787, 95% confidence interval, 1.020-3.133, p = 0.043). The transfection assay showed that overexpression of miR-125b in HCC cell line could obviously suppress the cell growth and phosporylation of Akt. In conclusion, the authors have demonstrated the diagnostic miRNA profile for HCC, and for the first time, identified the miR-125b with predictive significance for HCC prognosis.
Li
Wenxi
W
State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Shanghai Jiao Tong University, Shanghai, China.
Xie
Lu
L
He
Xianghuo
X
Li
Jinjun
J
Tu
Kang
K
Wei
Lin
L
Wu
Jun
J
Guo
Yong
Y
Ma
Xi
X
Zhang
Pingping
P
Pan
Zhimei
Z
Hu
Xin
X
Zhao
Yingjun
Y
Xie
Haiyang
H
Jiang
Guoping
G
Chen
Taoyang
T
Wang
Jianneng
J
Zheng
Shusen
S
Cheng
Jing
J
Wan
Dafang
D
Yang
Shengli
S
Li
Yixue
Y
Gu
Jianren
J
D015153:Blotting, Western; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002874:Chromosome Mapping; D006801:Humans; D008099:Liver / Q000201:enzymology / Q000378:metabolism; D008113:Liver Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D020411:Oligonucleotide Array Sequence Analysis; D010766:Phosphorylation; D011379:Prognosis; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction
D035683:MicroRNAs; D051057:Proto-Oncogene Proteins c-akt