title: High-speed gas chromatography: the importance of instrumentation optimization and the elimination of extra-column band broadening.
abstract: This review provides a summary of chromatographic theory as it applies to high-speed gas chromatography. A novel method for determining the optimal linear flow velocity, u (opt), from specific experimental parameters, is discussed. An in-depth theoretical understanding of u (opt) and its relation to experimental parameters is presented, in the absence of extra-column band broadening, as a means of method evaluation and optimization. Recent developments in high-speed GC are discussed, in the context of the theory presented within this review, to ascertain the influence of extra-column band broadening. The theory presented herein can be used as a means of evaluating the various areas of GC instrumentation (injection, separation, detection, etc.) that need further development to further minimize the effects of extra-column band broadening. The theoretical framework provided in this review, can be, and is, readily used to evaluate high-speed GC results presented in the literature, and thus, the general practitioner may more readily select a specific capillary length and/or internal diameter for a given application. For example, it is theoretically shown, and prior work cited, that demonstrates a peak width of approximately 1 ms is readily achievable in GC, when extra-column band broadening is eliminated.
authors.affiliation: Department of Chemistry, Box 351700, University of Washington, Seattle, Washington 98195-1700, USA.
authors.forename: Vanessa R
authors.initials: VR
authors.lastname: Reid
authors.forename: Robert E
authors.initials: RE
authors.lastname: Synovec
mesh_terms: D000818:Animals; D002630:Chemotactic Factors / Q000032:analysis / Q000302:isolation & purification; D002849:Chromatography, Gas / Q000379:methods*; D004058:Diffusion; D004867:Equipment Design; D007313:Insecta; D008855:Microscopy, Electron, Scanning; D008962:Models, Theoretical; D011312:Pressure; D013696:Temperature; D013997:Time Factors
chemical_list: D002630:Chemotactic Factors