title: Tumor targeting of humanized fragment antibody secreted from transgenic rice cell suspension culture.
abstract: The tumor-associated glycoprotein 72 (TAG 72) has been shown to be expressed in the majority of human adenocarcinomas. In an effort to develop a technique for the safe and inexpensive production of large quantities of anti-TAG 72 humanized antibody fragments (hzAb) as a future source of clinical-grade proteins, we developed a transgenic rice cell suspension culture system. The in vivo assembly and secretion of hzAb were achieved in a transgenic rice cell culture under the control of the rice alpha amylase 3D (RAmy 3D) expression system, and the biological activities of plant-derived hzAb were determined to be quite similar to those of animal-derived antibody. Purified hzAb was shown to bind to the recombinant antigen, TAG 72, and to bind specifically to human LS 174T colon adenocarcinoma cells expressing the TAG 72 antigen, and this binding occurred to the same extent as was seen with animal-derived antibody. Plant-derived hzAb proved as effective as animal-derived antibody in targeting tumors of xenotransplanted LS 174T cells in nude mice. The results of this study indicate that the hzAb derived from plant cell suspension cultures may have great potential for pharmaceutical applications in the development of future cancer therapeutic and diagnostic protocols.
authors.affiliation: Division of Biological Sciences, Research Institute for Bioactive Materials, Chonbuk National University, Jeonju 561-756, Korea.
authors.forename: Shin-Young
authors.initials: SY
authors.lastname: Hong
authors.forename: Tae-Sup
authors.initials: TS
authors.lastname: Lee
authors.forename: Ju
authors.initials: J
authors.lastname: Kim
authors.forename: Jae-Ho
authors.initials: JH
authors.lastname: Jung
authors.forename: Chang-Woon
authors.initials: CW
authors.lastname: Choi
authors.forename: Tae-Geum
authors.initials: TG
authors.lastname: Kim
authors.forename: Tae-Ho
authors.initials: TH
authors.lastname: Kwon
authors.forename: Yong-Suk
authors.initials: YS
authors.lastname: Jang
authors.forename: Moon-Sik
authors.initials: MS
authors.lastname: Yang
mesh_terms: D000818:Animals; D000951:Antigens, Neoplasm / Q000276:immunology*; D015153:Blotting, Western; D016466:CHO Cells; D018929:Cell Culture Techniques / Q000379:methods*; D045744:Cell Line, Tumor; D006224:Cricetinae; D003412:Cricetulus; D003470:Culture Media; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D005434:Flow Cytometry; D006023:Glycoproteins / Q000276:immunology*; D006801:Humans; D007128:Immunoglobulin Fragments / Q000276:immunology* / Q000302:isolation & purification; D051379:Mice; D008807:Mice, Inbred BALB C; D009077:Mucins / Q000378:metabolism; D009369:Neoplasms / Q000276:immunology* / Q000628:therapy*; D012275:Oryza / Q000166:cytology* / Q000235:genetics*; D030821:Plants, Genetically Modified; D011485:Protein Binding; D013347:Subcellular Fractions / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D000951:Antigens, Neoplasm; D003470:Culture Media; D006023:Glycoproteins; D007128:Immunoglobulin Fragments; D009077:Mucins; C047052:tumor-associated antigen 72