title: Structural and phylogenetic relationship of ORF 31 from the Anticarsia gemmatalis MNPV to poly (ADP-ribose) polymerases (PARP).
abstract: ORF 31 is a unique baculovirus gene in the genome of Anticarsia gemmatalis multiple nucleopolyhedrovirus isolate 2D (AgMNPV-2D). It encodes a putative polypeptide of 369 aa homologous to poly (ADP-ribose) polymerase (PARP) found in the genomes of several organisms. Moreover, we found a phylogenetic association with Group I PARP proteins and a 3D homology model of its conserved PARP C-terminal catalytic domain indicating that had almost an exact spatial superimposition of <1 A with other PARP available structures. The 5' end of ORF 31 mRNA was located at the first nucleotide of a CATT motif at position -27. Using real-time PCR we detected transcripts at 3 h post-infection (p.i.) increasing until 24 h p.i., which coincides with the onset of DNA replication, suggestive of a possible role in DNA metabolism.
authors.affiliation: Departamento de Microbiologia, Laboratório de Evolução Molecular e Bioinformática, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Juliana Velasco
authors.initials: JV
authors.lastname: de Castro Oliveira
authors.forename: Fernando Lucas
authors.initials: FL
authors.lastname: de Melo
authors.forename: Camila Malta
authors.initials: CM
authors.lastname: Romano
authors.forename: Atila
authors.initials: A
authors.lastname: Iamarino
authors.forename: Thais Sampaio
authors.initials: TS
authors.lastname: Rizzi
authors.forename: Fernanda Peres
authors.initials: FP
authors.lastname: Yeda
authors.forename: Charlotte Marianna
authors.initials: CM
authors.lastname: Hársi
authors.forename: José Luiz Caldas
authors.initials: JL
authors.lastname: Wolff
authors.forename: Paolo Marinho
authors.initials: PM
authors.lastname: de Andrade Zanotto
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002460:Cell Line; D008958:Models, Molecular; D008969:Molecular Sequence Data; D017924:Nucleopolyhedroviruses / Q000737:chemistry / Q000145:classification* / Q000201:enzymology* / Q000235:genetics; D016366:Open Reading Frames; D010802:Phylogeny; D011065:Poly(ADP-ribose) Polymerases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D018411:Spodoptera; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D014764:Viral Proteins; D011065:Poly(ADP-ribose) Polymerases