title: Effect of bisphenol A with or without enzyme treatment on the proliferation and viability of MCF-7 cells.
abstract: Recently, aqueous solutions polluted by BPA have been bioremediated by us using laccase immobilized on hydrophobic membranes in non-isothermal bioreactors. BPA degradation was checked using analytical methods. To assess in vitro the occurred bioremediation, the proliferation and viability indexes of MCF-7 cells incubated in the presence of aqueous solutions of BPA, or of enzyme-treated BPA solutions, have been measured as a function of the initial BPA concentration. The results demonstrated that: i) at each initial BPA concentration used, both the proliferation and viability indexes are a function of the duration of enzyme treatment; ii) proliferation and viability are uncoupled biological processes with respect to BPA enzyme treatment. Non-isothermal bioreactors are a useful tool for the bioremediation of aqueous solutions polluted by BPA, which is an example of an endocrine disruptor that belongs to the alkyl phenol family.
authors.affiliation: National Institute of Biosystems and Biostructures, Viale Medaglie d'Oro, 305, 00136 Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ricupito
authors.forename: G
authors.initials: G
authors.lastname: Del Pozzo
authors.forename: N
authors.initials: N
authors.lastname: Diano
authors.forename: V
authors.initials: V
authors.lastname: Grano
authors.forename: M
authors.initials: M
authors.lastname: Portaccio
authors.forename: M
authors.initials: M
authors.lastname: Marino
authors.forename: A
authors.initials: A
authors.lastname: Bolli
authors.forename: P
authors.initials: P
authors.lastname: Galluzzo
authors.forename: P
authors.initials: P
authors.lastname: Bontempo
authors.forename: L
authors.initials: L
authors.lastname: Mita
authors.forename: L
authors.initials: L
authors.lastname: Altucci
authors.forename: D G
authors.initials: DG
authors.lastname: Mita
mesh_terms: D001559:Benzhydryl Compounds; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects*; D052244:Endocrine Disruptors / Q000378:metabolism* / Q000633:toxicity*; D006801:Humans; D042845:Laccase / Q000378:metabolism*; D010636:Phenols / Q000037:antagonists & inhibitors / Q000378:metabolism* / Q000633:toxicity*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D042845:Laccase; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds