title: Transcriptional analyses before and after suppression of immediate hypersensitivity reactions by CCR3 blockade in eyes with experimental allergic conjunctivitis.
abstract: OBJECTIVE
  To characterize the transcriptome of allergic conjunctivitis mediated by eosinophil-related chemokine receptor CCR3 and to identify a candidate for possible therapeutic intervention in eosinophilic inflammation of the eye.
  
  
  METHODS
  Mice were sensitized to ragweed pollen, and allergic conjunctivitis was induced by an allergen challenge. The induction of allergic conjunctivitis was used to determine whether an inhibition of CCR3 would suppress eosinophilic inflammation and the allergen-induced immediate hypersensitivity reaction. In addition, sensitized mice were treated with a CCR3 antagonist or an anti-CCR3 antibody before the allergen challenge. Eosinophilic inflammation was evaluated histologically at 24 hours after the allergen challenge. Transcriptional changes with or without a blockade of CCR3 were determined by microarray analyses.
  
  
  RESULTS
  Blockade of CCR3 significantly suppressed allergen-induced clinical signs, mast cell degranulation, and eosinophilic inflammation. Clustering analysis of the transcriptome during the early phase identified clusters of genes associated with distinct biological processes. A CCR2 ligand, monocyte chemoattractant protein (MCP)-1, was identified in the cluster of genes related to mast cell activation. MCP-1, an attractant of monocytes but not eosinophils, was in the top 10 transcripts among the genome and was suppressed by CCR3 blockade. Importantly, antibody blockade of MCP-1 suppressed the eosinophilic inflammation significantly.
  
  
  CONCLUSIONS
  CCR3 regulates not only the eosinophilic inflammation but also the clinical signs and mast cell degranulation. The CCR3-mediated transcriptome is characterized by many biological processes associated with mast cell activation. Among these CCR3-mediated processes, MCP-1 was found to be significantly involved in eosinophilic inflammation probably by an indirect pathway.
authors.affiliation: Division of Ophthalmology and Visual Science, Faculty of Medicine, Tottori University, Totttori, Japan. nkomatsu0411@ybb.ne.jp
authors.forename: Naoki
authors.initials: N
authors.lastname: Komatsu
authors.forename: Dai
authors.initials: D
authors.lastname: Miyazaki
authors.forename: Takeshi
authors.initials: T
authors.lastname: Tominaga
authors.forename: Chuan-Hui
authors.initials: CH
authors.lastname: Kuo
authors.forename: Sachiko
authors.initials: S
authors.lastname: Namba
authors.forename: Shuzo
authors.initials: S
authors.lastname: Takeda
authors.forename: Hidemitsu
authors.initials: H
authors.lastname: Higashi
authors.forename: Yoshitsugu
authors.initials: Y
authors.lastname: Inoue
mesh_terms: D031164:Ambrosia; D000818:Animals; D002199:Capillary Permeability; D015550:Cell Degranulation; D002465:Cell Movement; D018932:Chemokine CCL2 / Q000502:physiology*; D003233:Conjunctivitis, Allergic / Q000235:genetics* / Q000276:immunology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology; D006969:Hypersensitivity, Immediate / Q000276:immunology / Q000517:prevention & control*; D007073:Immunoglobulin E / Q000097:blood; D007074:Immunoglobulin G / Q000097:blood; D008407:Mast Cells / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D020411:Oligonucleotide Array Sequence Analysis; D010880:Piperidines / Q000494:pharmacology; D011058:Pollen / Q000276:immunology; D054397:Receptors, CCR3 / Q000037:antagonists & inhibitors / Q000502:physiology*; D014158:Transcription, Genetic
chemical_list: C533770:4-(3-aminophenyl)thiazol-2-ylthio)-N-(1-(3,4-dichlorobenzyl)piperidin-4-yl; C491768:Ccl2 protein, mouse; C515351:Ccr3 protein, mouse; D018932:Chemokine CCL2; D007074:Immunoglobulin G; D010880:Piperidines; D054397:Receptors, CCR3; D007073:Immunoglobulin E