title: [Study of Tripterygium hypoglaucum Hutch on mouse's splenic lymphocyte activation in vitro].
abstract: OBJECTIVE
  To study the effects of Tripterygium hypoglaucum Hutch (THH) on the mouse's splenic lymphocyte proliferation, activation mark such as CD69 and CD25, cytokine such as IL-4 and gamma-IFN.
  
  
  METHODS
  Separating the mouse's splenic lymphocyte sterilitily and adding THH of different density. The proliferation of the ConA-stimulated splenic lymphocyte was measured with MTT; the activation marks such as CD69 and CD25 were measured with flow cytometer; cytokines such as IL-4 and gamma-IFN were measured with euzymelinked immunosorbent assay.
  
  
  RESULTS
  THH had inhibitory action on both proliferation and activation of splenic lymphocyte.
authors.affiliation: Department of Dermatology, First Affiliated Hospital of Kunming College, Kunming 650032, China. xuyanfirst@163.com
authors.forename: Yan
authors.initials: Y
authors.lastname: Xu
authors.forename: Yong-Tang
authors.initials: YT
authors.lastname: Zheng
authors.forename: Li
authors.initials: L
authors.lastname: He
authors.forename: Ping
authors.initials: P
authors.lastname: Wan
authors.forename: Xiang
authors.initials: X
authors.lastname: Nong
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology*; D005434:Flow Cytometry; D007371:Interferon-gamma / Q000378:metabolism; D015847:Interleukin-4 / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects*; D008297:Male; D051379:Mice; D010946:Plants, Medicinal / Q000737:chemistry; D013154:Spleen / Q000166:cytology*; D013997:Time Factors; D029757:Tripterygium / Q000737:chemistry*
chemical_list: D015703:Antigens, CD; D004365:Drugs, Chinese Herbal; D015847:Interleukin-4; D007371:Interferon-gamma