Gene expression profiling of lipoarabinomannan-treated mouse macrophage cultures infected with Mycobacterium bovis BCG.
The mannosylated lipoarabinomanan (ManLAM) from mycobacterial species possesses strong anti-apoptotic action. Here we examined the ability of ManLAM isolated from Mycobacterium tuberculosis H37Rv to alter expression profiles of apoptosis-related genes in mouse macrophages infected with Mycobacterium bovis BCG Danish strain. ManLAM suppressed BCG-induced apoptosis and activities of caspase-1, -3, -8 and 9. Mouse Apoptosis Gene Array showed that ManLAM significantly down-regulated pro-apoptotic and proinflammatory genes: caspase-1, -3, -7, -8 and -9, TNF-alpha/TNFSF2, Fas/TNFRSF6, Bax-alpha, as well as IL-12 p35 and iNOS simultaneously up-regulating anti-apoptotic genes such as Bcl-2 and Mcl-1. The effect of ManLAM was contrary to BCG-induced up-regulation of proapoptotic and pro-inflammatory genes and consistent with the functional data.
Krzyzowska
Małgorzata
M
Division of Immunology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - SGGW, Warsaw, Poland. malgorzata_krzyzowska@sggw.pl
Malewski
Tadeusz
T
Hamasur
Beston
B
Augustynowicz-Kopeć
Ewa
E
Schollenberger
Ada
A
Pawłowski
Andrzej
A
Niemiałtowski
Marek
M
D000818:Animals; D000942:Antigens, Bacterial / Q000378:metabolism; D017209:Apoptosis / Q000235:genetics*; D020169:Caspases / Q000378:metabolism; D002478:Cells, Cultured; D015536:Down-Regulation; D020869:Gene Expression Profiling; D051034:Inhibitor of Apoptosis Proteins / Q000378:metabolism; D008070:Lipopolysaccharides / Q000494:pharmacology*; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D051379:Mice; D008807:Mice, Inbred BALB C; D009163:Mycobacterium bovis / Q000276:immunology*; D009169:Mycobacterium tuberculosis / Q000737:chemistry; D020411:Oligonucleotide Array Sequence Analysis; D012313:RNA / Q000302:isolation & purification; D020133:Reverse Transcriptase Polymerase Chain Reaction; D013997:Time Factors; D015854:Up-Regulation
D000942:Antigens, Bacterial; D051034:Inhibitor of Apoptosis Proteins; D008070:Lipopolysaccharides; C050016:lipoarabinomannan; D012313:RNA; D020169:Caspases