Effective induction of high-titer antibodies by viral vector vaccines.
Protein-in-adjuvant vaccines have shown limited success against difficult diseases such as blood-stage malaria. Here we show that a recombinant adenovirus-poxvirus prime-boost immunization regime (known to induce strong T cell immunogenicity) can also induce very strong antigen-specific antibody responses, and we identify a simple complement-based adjuvant to further enhance immunogenicity. Antibodies induced against a blood-stage malaria antigen by this viral vector platform are highly effective against Plasmodium yoelii parasites in mice and against Plasmodium falciparum in vitro.
Draper
Simon J
SJ
The Jenner Institute, University of Oxford, Old Road Campus Research Building, Roosevelt Drive, Headington, Oxford OX3 7DQ, UK. simon.draper@ndm.ox.ac.uk
Moore
Anne C
AC
Goodman
Anna L
AL
Long
Carole A
CA
Holder
Anthony A
AA
Gilbert
Sarah C
SC
Hill
Fergal
F
Hill
Adrian V S
AV
D000256:Adenoviridae / Q000737:chemistry; D000818:Animals; D005822:Genetic Vectors / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry; D008288:Malaria / Q000517:prevention & control; D017780:Malaria Vaccines / Q000737:chemistry*; D051379:Mice; D008807:Mice, Inbred BALB C; D010963:Plasmodium falciparum / Q000378:metabolism; D010967:Plasmodium yoelii / Q000378:metabolism; D011212:Poxviridae / Q000737:chemistry; D013601:T-Lymphocytes / Q000469:parasitology / Q000821:virology*; D014612:Vaccines / Q000737:chemistry; D022223:Vaccines, Subunit / Q000737:chemistry; D014765:Viral Vaccines / Q000737:chemistry*
D007074:Immunoglobulin G; D017780:Malaria Vaccines; D014612:Vaccines; D022223:Vaccines, Subunit; D014765:Viral Vaccines