[Influence of cry2A sporulation-dependent promoter and molecular chaperone ORF1-ORF2 from Bacillus thuringiensis on Cry11Aa protein].
OBJECTIVE
To analyze the coordination function of cry2A sporulation-dependent promoter and the enhanced expression of molecular chaperone ORF1-ORF2 to crystal protein Cry11Aa.


METHODS
We constructed three recombinant plasmids pHcy1, pHcy2 and pHcy4 containing cry11Aa gene. pHcy1 carried cry11Aa own promoter and p19 gene, and pHcy2 carried cry2A sporulation-dependent promoter and orf1-orf2 gene. pHcy4 inserted cry2A promoter and orf1-orf2 gene upstream pHcy1 plasmid. The recombinant plasmids were introduced into an acrystalliferous mutant 4Q7 of Bacillus thuringiensis sub sp. israelensis. We performed SDS-PAGE to analyze Cry11Aa protein expression in the recombinant Bt strains and carried out the mosquitocidal bioassay.


RESULTS
SDS-PAGE showed that Cry11Aa protein was detected in 4Q7(pHcy1) and 4Q7(pHcy4), but not in 4Q7(pHcy2). The cry11Aa gene could not be regulated under cry2A promoter. Cry11Aa protein had a 1.25 fold expression amount in the equal volume culture of 4Q7(pHcy4) to that of 4Q7(pHcy1), which indicated that molecular chaperone ORF1-ORF2 could enhance Cry11Aa expression amount to a certain extent. Both 4Q7(pHcy1) and 4Q7(pHcy4) formed Cry11Aa crystals in a similar size and shape during sporulation under the transmission electron microscope. Their LC50s against 3rd-instar Culex quinquefasciatus were 59.33 ng/mL and 66.21 ng/mL respectively.


CONCLUSIONS
Whether crystal protein from B. thuringiensis could successfully express might relate to the type of the used promoter and their coordination. Molecular chaperone ORF1-ORF2 could enhance Cry11Aa expression amount to a certain extent with an unknown mechanism, but did not have an effect on high mosquitocidal toxicity of Cry11Aa protein. This research might play an important role to search the best collocation between ICP promoter or chaperone gene and ICP gene and to construct high-toxic Bacillus thuringiensis engineering strain by chaperone gene.
Shi
Yongxia
Y
State Key Laboratory for Biocontrol, Zhongshan (Sun Yat-sen) University, Guangzhou 510275, China. syx0817@yahoo.com.cn
Zeng
Shaoling
S
Yuan
Meijin
M
Sun
Fan
F
Pang
Yi
Y
D000818:Animals; D001413:Bacillus thuringiensis / Q000235:genetics* / Q000378:metabolism* / Q000648:ultrastructure; D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity; D003460:Crystallization; D003465:Culex / Q000187:drug effects; D004274:DNA, Recombinant / Q000235:genetics; D004591:Electrophoresis, Polyacrylamide Gel; D004731:Endotoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity; D015870:Gene Expression; D015964:Gene Expression Regulation, Bacterial; D005818:Genetic Engineering; D006460:Hemolysin Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism* / Q000633:toxicity; D046529:Microscopy, Electron, Transmission; D018832:Molecular Chaperones / Q000378:metabolism*; D016366:Open Reading Frames / Q000235:genetics*; D010957:Plasmids / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D013171:Spores, Bacterial
D000083722:Bacillus thuringiensis Toxins; D001426:Bacterial Proteins; D004274:DNA, Recombinant; D004731:Endotoxins; D006460:Hemolysin Proteins; D018832:Molecular Chaperones; C030956:insecticidal crystal protein, Bacillus Thuringiensis