title: Automated analysis of fluvoxamine in rat plasma using a column-switching system and ion-pair high-performance liquid chromatography.
abstract: We have established a robust, fully automated analytical method for the analysis of fluvoxamine in rat plasma using a column-switching ion-pair high-performance chromatography system. The plasma sample was injected onto a precolumn packed with Shim-pack MAYI-ODS (50 microm), where the drug was automatically purified and enriched by on-line solid-phase extraction. After elution of the plasma proteins, the analyte was back-flushed from the precolumn and then separated isocratically on a reversed-phase C18 column (L-column ODS) with a mobile phase (acetonitrile-0.1% phosphoric acid, 36:64, v/v) containing 2 mM sodium 1-octanesulfonate. The analyte was monitored by a UV detector at a wavelength of 254 nm. The calibration line for fluvoxamine showed good linearity in the range of 5-5000 ng/mL (r > 0.999) with the limit of quantification of 5 ng/mL (RSD = 6.51%). Accuracy ranged from -2.94 to 4.82%, and the within- and between-day precision of the assay was better than 8% across the calibration range. The analytical sensitivity and accuracy of this assay is suitable for characterization of the pharmacokinetics of orally-administered fluvoxamine in rats.
authors.affiliation: Research Department, Saitama Daiichi Pharmaceutical Co., Ltd, 8-1, Minamisakae-cho, Kasukabe, Saitama 344-0057, Japan. riu@sdsk.co.jp
authors.forename: Shicheng
authors.initials: S
authors.lastname: Liu
authors.forename: Norihiro
authors.initials: N
authors.lastname: Shinkai
authors.forename: Ikuhiro
authors.initials: I
authors.lastname: Kakubari
authors.forename: Hideo
authors.initials: H
authors.lastname: Saitoh
authors.forename: Ken-ichi
authors.initials: K
authors.lastname: Noguchi
authors.forename: Takashi
authors.initials: T
authors.lastname: Saitoh
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Yamauchi
mesh_terms: D000818:Animals; D002138:Calibration; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D016666:Fluvoxamine / Q000097:blood*; D051381:Rats; D015203:Reproducibility of Results
chemical_list: D016666:Fluvoxamine