title: Oral administration recombinant porcine epidermal growth factor enhances the jejunal digestive enzyme genes expression and activity of early-weaned piglets.
abstract: This study attempted to determine ingested porcine epidermal growth factor (pEGF) on the gastrointestinal tract development of early-weaned piglets. Thirty-two piglets (14-day weaned) were randomly allotted to supplemented with 0 (control), 0.5, 1.0, or 1.5 mg pEGF/kg diet. Each treatment consisted of four replicates with two pigs per pen for a 14 days experimental period. Piglets were sacrificed and gastrointestinal tract samples were collected to measure mucosa morphology, mRNA expression and activities of digestive enzymes in the gastrointestinal tract of piglets at the end of the experiment. Diets supplemented with pEGF failed to influence growth performance but tended to increase jejunal mucosa weight (p < 0.09) and protein content (p < 0.07). Piglets supplemental pEGF induced incrementally the gastric pepsin activity (p < 0.05) and stimulated jejunal alkaline phosphatase (ALP) and lactase activities accompanied with the increase of jejunal ALP and maltase mRNA expression. No effect of pEGF on the activities of all enzymes in ileum except the stimulation of ileal aminopeptide N mRNA expression. These results reveal that dietary pEGF supplementation might enhance gene expression and activities of digestive enzymes in the stomach and jejunum of piglets.
authors.affiliation: Department of Animal Science, National Ilan University, Ilan, Taiwan, Republic of China.
authors.forename: D N
authors.initials: DN
authors.lastname: Lee
authors.forename: Y S
authors.initials: YS
authors.lastname: Chuang
authors.forename: H Y
authors.initials: HY
authors.lastname: Chiou
authors.forename: F Y
authors.initials: FY
authors.lastname: Wu
authors.forename: H T
authors.initials: HT
authors.lastname: Yen
authors.forename: C F
authors.initials: CF
authors.lastname: Weng
mesh_terms: D000284:Administration, Oral; D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D004063:Digestion / Q000187:drug effects / Q000502:physiology; D004305:Dose-Response Relationship, Drug; D004815:Epidermal Growth Factor / Q000008:administration & dosage / Q000494:pharmacology*; D015971:Gene Expression Regulation, Enzymologic; D007413:Intestinal Mucosa / Q000187:drug effects; D007583:Jejunum / Q000201:enzymology* / Q000254:growth & development / Q000378:metabolism; D043322:Lactase / Q000378:metabolism; D010434:Pepsin A / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D013270:Stomach / Q000201:enzymology*; D013393:Sucrase / Q000378:metabolism; D013552:Swine; D014886:Weaning; D000520:alpha-Glucosidases / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D004815:Epidermal Growth Factor; D000469:Alkaline Phosphatase; D043322:Lactase; D000520:alpha-Glucosidases; D013393:Sucrase; D010434:Pepsin A