title: Modeling cellular deformations using the level set formalism.
abstract: BACKGROUND
  Many cellular processes involve substantial shape changes. Traditional simulations of these cell shape changes require that grids and boundaries be moved as the cell's shape evolves. Here we demonstrate that accurate cell shape changes can be recreated using level set methods (LSM), in which the cellular shape is defined implicitly, thereby eschewing the need for updating boundaries.
  
  
  RESULTS
  We obtain a viscoelastic model of Dictyostelium cells using micropipette aspiration and show how this viscoelastic model can be incorporated into LSM simulations to recreate the observed protrusion of cells into the micropipette faithfully. We also demonstrate the use of our techniques by simulating the cell shape changes elicited by the chemotactic response to an external chemoattractant gradient.
  
  
  CONCLUSIONS
  Our results provide a simple but effective means of incorporating cellular deformations into mathematical simulations of cell signaling. Such methods will be useful for simulating important cellular events such as chemotaxis and cytokinesis.
authors.affiliation: Electrical & Computer Engineering, Johns Hopkins University, Baltimore, MD 21218, USA. liuyang@jhu.edu
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.forename: Janet C
authors.initials: JC
authors.lastname: Effler
authors.forename: Brett L
authors.initials: BL
authors.lastname: Kutscher
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Sullivan
authors.forename: Douglas N
authors.initials: DN
authors.lastname: Robinson
authors.forename: Pablo A
authors.initials: PA
authors.lastname: Iglesias
mesh_terms: D000818:Animals; D001696:Biomechanical Phenomena; D002462:Cell Membrane / Q000378:metabolism; D048430:Cell Shape; D048429:Cell Size; D002633:Chemotaxis; D003599:Cytoskeleton / Q000378:metabolism; D004023:Dictyostelium / Q000166:cytology; D004548:Elasticity; D008954:Models, Biological; D011312:Pressure; D012680:Sensitivity and Specificity; D014783:Viscosity