title: Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex.
abstract: Yeast RNA polymerase (Pol) II consists of a 10-subunit core enzyme and the Rpb4/7 subcomplex, which is dispensable for catalytic activity and dissociates in vitro. To investigate whether Rpb4/7 is an integral part of DNA-associated Pol II in vivo, we used chromatin immunoprecipitation coupled to high resolution tiling microarray analysis. We show that the genome-wide occupancy profiles for Rpb7 and the core subunit Rpb3 are essentially identical. Thus, the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle.
authors.affiliation: Gene Center and Center for Integrated Protein Science Munich CIPSM, Department of Chemistry and Biochemistry, Ludwig-Maximilians-University of Munich, Feodor-Lynen-Strasse 25, D-81377 Munich, Germany.
authors.forename: Anna J
authors.initials: AJ
authors.lastname: Jasiak
authors.forename: Holger
authors.initials: H
authors.lastname: Hartmann
authors.forename: Elena
authors.initials: E
authors.lastname: Karakasili
authors.forename: Marian
authors.initials: M
authors.lastname: Kalocsay
authors.forename: Andrew
authors.initials: A
authors.lastname: Flatley
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Kremmer
authors.forename: Katja
authors.initials: K
authors.lastname: Strässer
authors.forename: Dietmar E
authors.initials: DE
authors.lastname: Martin
authors.forename: Johannes
authors.initials: J
authors.lastname: Söding
authors.forename: Patrick
authors.initials: P
authors.lastname: Cramer
mesh_terms: D016681:Genome, Fungal / Q000502:physiology*; D012319:RNA Polymerase II / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000502:physiology*
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D012319:RNA Polymerase II