[Study on relationship between intratumoral lymphangiogenesis of laryngeal squamous cell carcinoma and lymphatic metastasis].
OBJECTIVE
To elucidate the relationship between the intratumoral lymphangiogenesis and lymphatic metastasis, and provide some theoretic evidence for the judgement of lymph node metastasis and prognosis of laryngeal squamous cell carcinoma (LSCC), also for the treatment.


METHODS
Immunohistochemical analysis was performed to 50 specimens of LSCC with lymphatic endothelial marker (Lymphatic vessel endothelial hyaluronan receptor-1 LYVE-1), the vascular endothelial marker CD34 and the pKi67 proliferation marker to record lymphatic vessel density (LVD). Quantitation of lymphangiogenesis growth factor VEGF-C by RT- PCR was performed to 30 specimens of LSCC. Finally the correlation between LVD and tumor TNM grade, VEGF-C mRNA, grade of diffraction was analyzed with statistics methods.


RESULTS
Newly proliferating lymphatic vessel were observed in all LSCC. The median copy number of VEGF-c mRNA was 4-5-fold higher in LSCC than in adjacent normal tissue. There was correlation between tumor VEGF-C mRNA copy number and intratumoral LVD (n =30, P <0.05), there was no significant association between LVD and sex, T stage and grade of diffraction (n =50, P >0.05) but N stage (n = 50, P <0.05).


CONCLUSIONS
Newly proliferating lymphatic vessels existed in LSCC. There was correlation between high levels of LVD in LSCC than in normal tissue. And the high level of VEGF-C may accelerate the lymphatic metastasis by promoting the proliferation of intratumoral lymphatic.
Huangfu
Hui
H
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. huangfu83@yahoo.com.cn
Kong
Weijia
W
Gong
Shusheng
S
Wang
Binquan
B
Zhang
Chunming
C
D000328:Adult; D000368:Aged; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000473:pathology*; D005260:Female; D006801:Humans; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D042583:Lymphangiogenesis; D008207:Lymphatic Metastasis; D042601:Lymphatic Vessels / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D042582:Vascular Endothelial Growth Factor C / Q000378:metabolism
C467486:VEGFC protein, human; D042582:Vascular Endothelial Growth Factor C