Complementary analysis of microRNA and mRNA expression during phorbol 12-myristate 13-acetate (TPA)-induced differentiation of HL-60 cells.
MicroRNAs (miRNAs) and mRNAs constitute an important part of gene regulatory networks, influencing diverse biological phenomena. To discover novel regulatory pathways during myeloid differentiation, we performed miRNA as well as mRNA expression profiling of in vitro-differentiating HL-60 cells treated with 12-O-tetradecanoylphorbol-13-acetate (TPA). The main findings were up-regulation of miR-146a/b, miR-21, miR-221, miR-222, miR-155, miR-26a and down-regulation of miR-199a*, miR-181c, miR-142-3p, miR-92. After integrating the miRNA and mRNA expression data into a Transcriptome Interaction Database by Molecule Annotation System (MAS) software, a number of differently expressed mRNAs were revealed as potential targets of these miRNAs.
Chen
Ailiang
A
Medical Systems Biology Research Center, Tsinghua University School of Medicine, Beijing, 100084, Peoples' Republic of China.
Luo
Mingyong
M
Yuan
Guohua
G
Yu
Jian
J
Deng
Tuo
T
Zhang
Liang
L
Zhou
Yuxiang
Y
Mitchelson
Keith
K
Cheng
Jing
J
D002454:Cell Differentiation; D019070:Cell Lineage; D019295:Computational Biology; D015536:Down-Regulation; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D018922:HL-60 Cells; D006801:Humans; D008264:Macrophages / Q000166:cytology*; D035683:MicroRNAs / Q000378:metabolism*; D009000:Monocytes / Q000166:cytology*; D020411:Oligonucleotide Array Sequence Analysis; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000378:metabolism*; D013755:Tetradecanoylphorbol Acetate / Q000494:pharmacology*; D015854:Up-Regulation
D035683:MicroRNAs; D012333:RNA, Messenger; D013755:Tetradecanoylphorbol Acetate