Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos.
A novel gene-K23, differentially expressed in cross-subfamily cloned embryos, was isolated by RACE-PCR technique. It had 2580 base pairs (bp) in length, with a 1,425 bp open reading frame (ORF) encoding a putative protein of 474 amino acids (aa). Bioinformatic analysis indicated that K23 had 22 phosphorylation sites, but it had no signal peptides. Developmental expression analysis in zebrafish showed that K23 transcripts were maternally expressed in ovum and the amount of K23 transcripts increased gradually from zygote to pharyngula period. Subcellular localization analysis revealed that K23 protein was homogeneously distributed both in nuclei and cytoplasm. Taken together, our findings indicate that K23 gene is a novel gene differentially expressed in fish cross-subfamily cloned embryos.
Pei
De-Sheng
DS
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Sun
Yong-Hua
YH
Zhu
Zuo-Yan
ZY
D000818:Animals; D001483:Base Sequence; D002999:Clone Cells; D019295:Computational Biology; D004625:Embryo, Nonmammalian; D005260:Female; D040461:Gene Components; D018507:Gene Expression Regulation, Developmental; D050437:Genes, Developmental; D008969:Molecular Sequence Data; D010063:Ovum; D010750:Phosphoproteins / Q000235:genetics* / Q000378:metabolism; D010766:Phosphorylation; D016133:Polymerase Chain Reaction; D012333:RNA, Messenger / Q000032:analysis; D015027:Zebrafish / Q000235:genetics*; D029961:Zebrafish Proteins / Q000235:genetics* / Q000378:metabolism
C542797:K23 protein, zebrafish; D010750:Phosphoproteins; D012333:RNA, Messenger; D029961:Zebrafish Proteins