Autoantibodies as potential biomarkers for nasopharyngeal carcinoma.
Autoantibody signatures, as new biomarkers, may improve the early detection of nasopharyngeal carcinoma (NPC). We constructed a T7 phage cDNA library from mixed NPC tissues, and we isolated 31 tumor-associated proteins using biopan enrichment techniques with sera from NPC patients and from healthy population. DNA sequence analysis showed that among 31 phage-displayed proteins, 22 have sequence identity with known or putative tumor-associated proteins. The results of immunochemical reactivity of patients' sera with phage-expressed proteins showed enrichment in the number of immunogenic phage clones in the biopanning process and also confirmed that antibodies were present in the sera of patients but not in the sera of healthy donors. The autoantibody against phage-expressed protein MAGE, HSP70, Fibronectin, and CD44 measured by ELISA had greater predictive value than that against EBNA-1, respectively. The antibody levels against MAGE in sera positively correlated with the clinical stages of NPC, and the antibody levels against other three proteins partly correlated with the clinical stages of NPC. Our studies suggested that the autoantibodies against tumor-associated antigens in the sera of NPC patients could be used as a screening test for NPC. Studies of the corresponding proteins may have significances in tumor biology, novel drug development, and immunotherapy.
Tong
Yong-Qing
YQ
Cancer Research Institute, Xiang-Ya School of Medicine, Central South University, Hunan, PR China.
Zhang
Zhi-Jie
ZJ
Liu
Bei
B
Huang
Jian
J
Liu
Hui
H
Liu
Yan
Y
Guo
Feng-Jie
FJ
Zhou
Guo-Hua
GH
Xie
Ping-Li
PL
Li
Yue-Hui
YH
Zuo
Chao-Hu
CH
Hu
Jin-Yue
JY
Li
Guan-Cheng
GC
D000328:Adult; D001323:Autoantibodies / Q000032:analysis* / Q000276:immunology; D017123:Bacteriophage T7 / Q000235:genetics; D014408:Biomarkers, Tumor / Q000032:analysis* / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015723:Gene Library; D006801:Humans; D007118:Immunoassay; D008297:Male; D008875:Middle Aged; D009303:Nasopharyngeal Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000378:metabolism*; D019151:Peptide Library; D020543:Proteome / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
D001323:Autoantibodies; D014408:Biomarkers, Tumor; D019151:Peptide Library; D020543:Proteome