SELEX with modified nucleotides.
Aptamers, a promising new class of therapeutics, are single-stranded oligonucleotides generated via an in vitro selection process that bind to and inhibit the activity of target proteins in a manner similar to therapeutic antibodies. In order to enhance the drug-like character of aptamers, oligonucleotide libraries containing modified nucleotides are increasingly being used for selection. Principally, the choice of modifications aims to increase aptamer potency by enhancing nuclease-resistance, or increasing target affinity by providing more target recognition functionality or generating more stable aptamer structures.
Keefe
Anthony D
AD
Archemix Corp., 300 Third Street, Cambridge, MA 02142, USA. keefe@archemix.com
Cload
Sharon T
ST
D015723:Gene Library; D006801:Humans; D009713:Nucleotidyltransferases / Q000378:metabolism; D009841:Oligonucleotides / Q000737:chemistry* / Q000378:metabolism; D052156:SELEX Aptamer Technique / Q000379:methods*
D009841:Oligonucleotides; D009713:Nucleotidyltransferases