Detection of cortisol in saliva with a flow-filtered, portable surface plasmon resonance biosensor system.
Saliva provides a useful and noninvasive alternative to blood for many biomedical diagnostic assays. The level of the hormone cortisol in blood and saliva is related to the level of stress. We present here the development of a portable surface plasmon resonance (SPR) biosensor system for detection of cortisol in saliva. Cortisol-specific monoclonal antibodies were used to develop a competition assay with a six-channel portable SPR biosensor designed in our laboratory. The detection limit of cortisol in laboratory buffers was 0.36 ng/mL (1.0 nM). An in-line filter based on diffusion through a hollow fiber hydrophilic membrane served to separate small molecules from the complex macromolecular matrix of saliva prior to introduction to the sensor surface. The filtering flow cell provided in-line separation of small molecules from salivary mucins and other large molecules with only a 29% reduction of signal compared with direct flow of the same concentration of analyte over the sensor surface. A standard curve for detection of cortisol in saliva was generated with a detection limit of 1.0 ng/mL (3.6 nM), sufficiently sensitive for clinical use. The system will also be useful for a wide range of applications where small molecular weight analytes are found in complex matrixes.
Stevens
Richard C
RC
Department of Medicine, Division of Medical Genetics, University of Washington, Seattle, Washington 98195, USA.
Soelberg
Scott D
SD
Near
Steve
S
Furlong
Clement E
CE
D002021:Buffers; D006801:Humans; D006854:Hydrocortisone / Q000032:analysis* / Q000737:chemistry; D008970:Molecular Weight; D012463:Saliva / Q000737:chemistry*; D020349:Surface Plasmon Resonance / Q000379:methods*; D013499:Surface Properties
D002021:Buffers; D006854:Hydrocortisone